LOCUS       DASUTG010000001         5537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1482465, whole genome shotgun sequence.
ACCESSION   DASUTG010000001 DASUTG010000000
VERSION     DASUTG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482465"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..972)
                     /locus_tag="VF089_00005"
     CDS             complement(73..972)
                     /locus_tag="VF089_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6172362.1"
                     /translation="MEKCRFGKTDMNVSVLGFGGSEIGYERASLASVSKLLNRALDAG
                     VNVIDTAECYVTSEELIGNAISARRGDYYLFTKCGHERGWSYPDWHPSSLWKSIERSL
                     RRLKTDHVDLLQLHSCSEDELRKGEVIEFLKRARERGYTRFIGYSGDGQAALYAVNSG
                     EFDTLQTSINIADQEALDLTLPLAKKRNMGVIAKRPVANVAWADGDRPPRNGYGYPYW
                     ERLQKLKYDFLRGDLRDSVATALRFVLTVSGVHTAIVGTTKPQRFSENAQALEAGTLP
                     VEVFENVRSRWREIAGSDWVGQT"
     gene            complement(1257..1877)
                     /locus_tag="VF089_00010"
     CDS             complement(1257..1877)
                     /locus_tag="VF089_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172363.1"
                     /translation="MKQTWPLGIVGILFITLALNACSTATPIVSEWRNPAQTSGSFQR
                     LMIGGPSGDASVRRTFEDEFVAQLAAIGADALPSYRYMPENEVTNENNLKQAAQKARV
                     DGLLLMRSVSVEEKTNYPAIGPGISFGIFGSDVGAAWSGVSGTSGPYRYNEYTSETAL
                     YDVAKNELVWTGTLKTKEPTNVQAAIRSYVQTITKALAAQNLFPKR"
     gene            2416..3636
                     /locus_tag="VF089_00015"
     CDS             2416..3636
                     /locus_tag="VF089_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172364.1"
                     /translation="MKAWRWIYVGILVLSLDVGLGVAADDGQDIKDAKIAPRFRVDPS
                     WPKPLPDNWVIGQIGGIAVDSRDHVWVLQRPRSVPERYLGPTLTPPRSTCCFAAPSVI
                     EFDSKGEVVQAWGGPSDPGFLESRCTPAMGCDWPQTEHGIFVSDDGHVYIGGNGGPDH
                     QVIKFTGDGTFVMQIGIAGMRGSSHPVQFAPNGTPLLGRPAEMDLDRAANELYIADGY
                     QNRRIIVVDATTGQYKRHWGAYGNVPNDDDPGPYDPNAPLAQQFRTPVHCVAVASDGL
                     VYVCDRVNNRIQVFQKNGIFVTEFFTDRNTRGNGSAYNAALSPDRKQTYLFNADGENQ
                     HIWTFLRRTGEALTKTGRGGRFPGHFAAVHSLAVDSRGNVYTGEVDAGMRLQKFSNLG
                     NRPIRSDGRSDDDD"
     gene            4094..5089
                     /locus_tag="VF089_00020"
     CDS             4094..5089
                     /locus_tag="VF089_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6172365.1"
                     /translation="MTKFNARRCFLLVALIGITAIAGFSGRAGAQVKTRLSIATGGTG
                     GVYYPLGGGLAALISKHIPGVEATAEVTTASVDNMKLLHANRVALAFTLPDSALQAYQ
                     GQLKGMKEKIAVRTLAAIYSNYMHIVAIDGIGIKTVADLKGKRVSTGAPGSGTEIIAL
                     RVLETYGISPNDLRSQDRLGAAESAGALKDRKIDAFFWDGGLPTAAVLDLGATPGITI
                     RLIPHGEAVPKMVAKHGPLYFVRNIPKETYGGMDADIPVATATNLLAVHERLEEPMAY
                     QITKLLHEHTAELVAVHPAAKEISLKSAVTGSPIPFHPGALRFYKEKGVKVPDVK"
     gene            5191..>5537
                     /locus_tag="VF089_00025"
     CDS             5191..>5537
                     /locus_tag="VF089_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172366.1"
                     /translation="MADATAHHPIGMGDKLYIIFPHSIYGSQVEEHFRATSEGFQLFE
                     LRYAEPRLVEFYGHEWAANEDGRWVVRKRGPLLAILDLRVSPGSWINVIFGTEKLTVR
                     HDSLTHGRARLAL"
BASE COUNT         1192 a         1541 c         1561 g         1243 t
ORIGIN      
        1 gggaatttgt caggcttgtt ttcgtgagaa gaattcgagc ttggctttgg atggcgccgt
       61 gcaaagggta tctcaagttt gccccaccca gtcggaaccg gcaatttcgc gccagcggct
      121 gcgaacgttt tcaaagactt cgacgggcaa tgttcctgcc tccaaggcct gcgcattttc
      181 cgaaaacctc tgaggcttgg tggtgccgac aatcgccgta tgaacgcctg agacggtgag
      241 cacaaagcgc agcgcagttg ccacggagtc gcgcagatcg ccgcgcagaa aatcgtactt
      301 gagtttctgc agtcgttccc aatagggata gccgtaaccg ttacgagggg gccggtcgcc
      361 atcggcccaa gcgacgtttg ccaccggccg cttcgcaatt acccccatgt ttcgtttctt
      421 tgccagaggc aatgttagat cgagcgcttc ctggtcagca atgttgatag acgtctgcag
      481 cgtatcaaac tcgccgctgt taaccgcata cagtgctgct tggccgtcgc cgctgtaccc
      541 gatgaagcgg gtgtagccac gctcgcgtgc tcgcttgaga aactcgatca cttcaccttt
      601 acgcagctcg tcttccgaac agctgtggag ctgtagcaag tcgacatgat ccgtcttcag
      661 ccgtctaagg cttctctcga tagatttcca aagcgaagac gggtgccaat ccggataact
      721 ccatcctcgt tcatggccgc atttggtaaa gagatagtaa tcgcctcgtc gagcgctgat
      781 cgcgtttccg atcaattcct cactggtgac gtaacactcc gccgtatcga tgacgttgac
      841 tccggcatcg agagcacgat tcaatagttt gctcacagag gcgagtgatg ctcgctcata
      901 gccgatctcc gagccgccga agccgagaac gctaacattc atatcggttt tgccaaaccg
      961 gcatttttcc atttttttac ctttgaagga cagtgcccac gacagtgggt caggtattgc
     1021 aacgacacat gtgccacttt ccagaggcgc ccataccacc caactttttt tgctcatcgg
     1081 gtcgaatgca attcactcga ttttcggttt ctcttttaga taatacttcg agagcagcgg
     1141 tcggtaacgc tccatgagtt ccttattgag ataggcggcg gcttttggtc tggggtcact
     1201 tccgccacgt gccaagctat gcacggagac cccacgccta tacgcggcgt tcccaactat
     1261 cttttcggga acagattctg cgcggcgagc gctttcgtga tcgtttgcac ataggacctt
     1321 atcgccgctt gcacatttgt cggctccttc gtcttgaggg tgccggtcca caccaattcg
     1381 ttcttggcga catcgtagag cgcagtctcc gaggtgtatt cgttgtaacg gtaagggccg
     1441 gatgtcccgg aaactccgga ccaagcagcg ccgacatctg agccgaagat cccgaacgaa
     1501 attcccggtc ctattgcagg atagttggtc ttctcttcca cactgactga tcgcatcaag
     1561 agcaagccgt cgacgcgagc cttttgcgcg gcctgtttga ggttgttttc attcgtcact
     1621 tcattctccg gcatgtagcg gtagctaggc aatgcgtcgg cgccgattgc cgctagttga
     1681 gccacgaatt cgtcttcgaa ggtacgccga acgctggcat ctccgctcgg gcctccgatc
     1741 atcagtcgct ggaaggagcc cgaagtctgc gcgggattac gccattcact gacgattggg
     1801 gtagcggtcg aacaagcgtt gagagcgagg gtgatgaaaa gaattcccac aatgcctaag
     1861 ggccacgtct gcttcatgac ccatcatggc accaaatcaa cggttgttta agaacctccc
     1921 tgaaagttcg gtcgcgaact ttagtaagca cacgtgccag tgggtagacg attcactcat
     1981 gtgtgagagg atggcgagag aagatgtctc ggcatttagc tcgcttggga cctcctccgg
     2041 cgccttgctc aatatgaaca caaacaatcg gcgcccggat atgtggactt tgatcgatgc
     2101 ttatacgtta tgcacccaaa ttccctcgcg cgataacttg gaggtatgtg agaatcgcgg
     2161 tgaaacgtcc actgaaattg taggcatcca tcggtgatgc cataagaaat actaatgcca
     2221 gtcatcaaaa aacagtattg gacaacctct tgggctgtca gatacgagat gatctccaca
     2281 tcgacatctt tgcggactgc tttttatcgg acttgctatc gagctgtcga gagtcggctc
     2341 gcgcgcggct gagaaagcgg gtttcagtat ccgcgtcggg ctctacatca cccataaccc
     2401 gcaaaggaga tcgctatgaa agcttggcga tggatatacg ttggcatcct ggtgctctcg
     2461 ctggacgtcg gactgggggt cgccgcagac gacgggcaag acattaaaga tgcgaagatc
     2521 gcgccacgat ttagggtgga cccgtcatgg cccaagcctt tgccggacaa ctgggttatc
     2581 ggacagatcg gcggaatcgc cgtcgattcg cgtgatcacg tctgggttct gcagcgcccg
     2641 cgctctgtgc ccgagcgtta tctcggcccg acgctgaccc cgccgcgctc gacgtgctgc
     2701 tttgcggcgc cctccgtgat tgagttcgat tccaaaggcg aggtggtcca ggcgtgggga
     2761 ggcccatcgg atcccgggtt tctggaatca cgctgcacgc ccgcgatggg atgtgactgg
     2821 ccgcaaaccg agcacggcat cttcgtctcc gatgatggcc acgtctatat tggcggtaac
     2881 ggcggaccgg accaccaggt gatcaagttc accggcgacg gcacgttcgt gatgcagatc
     2941 ggcattgccg gcatgcgcgg cagcagtcac ccggtacagt ttgcgccgaa tgggacaccg
     3001 cttctcggcc gtccggccga gatggacctg gaccgcgccg ccaacgagct ctacattgcc
     3061 gacggttacc agaatagacg catcatcgtg gtcgacgcga ccactgggca gtacaagcgc
     3121 cactggggcg cctacggtaa cgtgccgaac gacgatgatc cgggcccgta tgatcccaac
     3181 gcaccgctcg ctcagcagtt ccgcacgcca gttcactgtg tggcagtggc gagcgatggt
     3241 ctggtatacg tctgcgatcg cgtcaacaac cggattcagg tgtttcagaa gaacgggatt
     3301 ttcgtcacgg aattcttcac tgaccgcaac acccgcggca acggctcggc atacaacgca
     3361 gcgctgtcgc ccgatagaaa gcagacgtat ctcttcaatg ccgacggcga gaaccagcat
     3421 atctggacgt ttttgcggcg cacgggcgaa gcgctgacaa agacgggccg tggcggcaga
     3481 ttccccggac acttcgccgc ggtgcacagc ctcgcggtgg attccagggg caacgtctac
     3541 acgggagaag tggatgcagg gatgaggctg cagaaattca gcaatctcgg caatcgcccg
     3601 atcagatccg acggccgcag cgatgatgac gactgaaaag acgctggggg agtttgcatg
     3661 atcttcctct tccccctgct gggaagaggt cagatacgag aggaagtctg ccttccacat
     3721 gatgcggagc ggcatgcgtc gagttgcttg ggaacggttc gacgggctgg gtccgtctgg
     3781 cctgcagcaa cgtgttcgat gccgccccgc atgcatcgtg atatcaacct cccttctttg
     3841 ccgcgtgtat gagttcgcta tttaaatcgt agccctgacc gggcgtgtta cttaccgcga
     3901 agcccggccg tgacgcggtt tggattcgct gatccggccg ggtcacggca acgattggac
     3961 acgcgcgcgc aagacactga cccaccgaag tgccttcgca aagaaccttg aagtcgacgc
     4021 ccgacagtgg ctttactcct tgtatgtgat cagagtatct gatcagagaa aaaactcaca
     4081 gctaggaggt aatatgacga aatttaatgc gcggcgatgt tttctgctcg tagcgctaat
     4141 cggaattact gcaatcgctg gtttctctgg gcgggcaggc gcccaggtaa aaacccggct
     4201 ttcaatcgcc accggcggaa ccggcggtgt ctattatccg ctgggcggcg gccttgccgc
     4261 gctgatctcg aagcatattc ccggggtgga ggccacggcg gaagtgacca ccgcctccgt
     4321 cgacaacatg aagcttctgc atgccaatag agtcgctctg gcgtttacat tgcccgatag
     4381 cgctctgcag gcctatcaag gacaattgaa gggtatgaaa gaaaaaattg cggttcgtac
     4441 cctagcggcc atttactcca actacatgca cattgttgcg atcgacggaa ttggcattaa
     4501 aacggtggcg gatctcaaag gaaagcgagt ttccactggg gctccgggtt ctggaacgga
     4561 aataatagcc ctgcgcgttc tcgagactta tggaatcagc cccaacgatc taagaagtca
     4621 ggaccgcttg ggcgccgcgg agtccgccgg cgcattgaag gatcgaaaga ttgacgcttt
     4681 cttctgggat ggcggtttgc cgacggcggc cgttctcgat ttgggagcca caccgggaat
     4741 tacgattcgc ctgattccac atggcgaggc cgtaccaaag atggtcgcaa agcatggtcc
     4801 cctatacttt gtccggaaca ttccaaaaga gacttacggc gggatggatg ccgacatccc
     4861 cgttgcaacc gcaactaatc tgttggcggt tcacgaacgc ttggaggagc cgatggccta
     4921 tcagatcacc aaattgctgc atgagcatac ggcggagctg gtggcggttc atccggccgc
     4981 caaggaaatc agtctcaaaa gcgcggtgac gggctctccg attccgttcc accccggcgc
     5041 gctgcgcttc tacaaggaaa aaggcgtcaa agttccggat gtcaaatgat gttttctggc
     5101 ccgcgaagaa tagcaggatt tttttctctg ttcttcggat gttttctggt caccggagta
     5161 ggagcggaat caatctgctt gaacgtgcag atggccgacg caactgcgca tcacccgatc
     5221 ggcatgggtg acaaacttta cataattttt ccccattcca tttacggctc ccaagtcgaa
     5281 gaacatttcc gcgccacttc agaaggattt cagcttttcg agctacgcta tgccgagcca
     5341 cgcctagtcg aattttatgg ccatgagtgg gctgctaacg aagacggccg gtgggtcgtc
     5401 cggaagcgcg gccctctttt ggcgatcctg gaccttcgcg taagcccagg gtcttggatc
     5461 aacgtcatct tcggcactga aaagctgacg gtaagacatg attcgttgac ccacggccgt
     5521 gctcgactgg cgttaag
//
LOCUS       DASUTG010000002         5780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_205, whole genome shotgun sequence.
ACCESSION   DASUTG010000002 DASUTG010000000
VERSION     DASUTG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VF089_00030"
     CDS             <1..912
                     /locus_tag="VF089_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6172367.1"
                     /translation="IFFENYAPGVAERLGLGYEKLSEINPRLIYCSLSGYGQNGPYRD
                     VKAYDLLIQGEGGIIATTGYPDKPAKASIAIADIASGMYAVIGIVLALYQRDKTGEGQ
                     LIDVSMLDSIVSWLGYFPHHYWHAGEEPGRAGMRHHYVTPYGPYLAADGEYVNLAVAS
                     PADWEQFCRVVIEKPELLEDSRFRTVQDRRKNRGSLEEIIEAIFLERDHKHWLAQLKK
                     AEMPHGIVRGIAQVLAHPQVAARKLIREADSPVGKVPVIANALKMSDSPARYDRIPEL
                     GEDNDGILQELGYDAAAIADLRRDKVI"
     gene            1318..1869
                     /locus_tag="VF089_00035"
     CDS             1318..1869
                     /locus_tag="VF089_00035"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172368.1"
                     /translation="MTKPVIGVMGASESDALTDAEKARLKQLAEKLGAEIAKHHCILI
                     SGATTGLPDLISRCAREHGGFAVGISPAENRREHIQRYKMPPDAADLLIYTGFGLKGR
                     NVVNVRSADIVIIFGGATGTLNEFTIAYDEGKIIGVLEGSGGCADHIKEIIEFCKKPT
                     RAIVLFDSDPEKLIEDCLKCLDK"
     gene            complement(2110..2991)
                     /locus_tag="VF089_00040"
     CDS             complement(2110..2991)
                     /locus_tag="VF089_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6172369.1"
                     /translation="MTDPKFIDVDGIRTRYFDKGSGEVLLLVHGSHMGTPDACESAID
                     WEFNFDVLAQSFRVIAMDKLGQGYTGNPKSDADYTMAATVKHAAGLLQTLGLQKVHVI
                     GHSRGGYVVTRLTLDHPELVRSCVIVDSGTLAPGPSKTEFIMANAPKPRLSRDSQRWC
                     IEHYSFRANHITQNWLDTCEDIAKSERYQQTVRKMEDEGLKINQFFTHLALHKEETLN
                     WIMHGKLTQPTLLVWGYNDPTAELKRGHALFEIIADSNPKSEMHIINEAGHFCYREQP
                     ETFNAIVKAFIEKSARL"
     gene            complement(3068..4090)
                     /locus_tag="VF089_00045"
     CDS             complement(3068..4090)
                     /locus_tag="VF089_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6172370.1"
                     /translation="MENLEPKGAAHWIDHFVVGTNDIVAWADWAVNGIGLTRRPFRGL
                     TTAERKRNMKVICFLWWEGGSCRIGAFLQPEIFPPAKELGQELPRCGFYIRQEDLDMH
                     LRRLDRYKIPHSDPLETCAEGDQGTVIYFADPDGNQYEFWAPAQMPVGAMEIATDERV
                     GRISHAVYGCRDLARTAAFFEKYCGLQADQKSTTSEGTLVLRLRAGARLIYKLVDKVD
                     ERVAGHGPWWDMHTALTVREQEFFPNYRRMWDGLPEEEADKANANLSLEQQEALPART
                     GLHRSPVGLKWKEICERGDEFYDWDAHAFHFIGGIPLKDDGSMALYQAKEQEVYLREL
                     AESLKK"
     gene            complement(4503..4841)
                     /locus_tag="VF089_00050"
     CDS             complement(4503..4841)
                     /locus_tag="VF089_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6172371.1"
                     /translation="MSNLTLHPMKEIRIIIQGEHLKFATDLLDNKATGYTIIHNISGK
                     GHHGFHTAHPMFNEMDSIVMLMSVVAPERVEPILAGLKPLFDRYTGVMFVSDVAVSRV
                     EYFSPGSAQK"
     gene            complement(4854..>5780)
                     /locus_tag="VF089_00055"
     CDS             complement(4854..>5780)
                     /locus_tag="VF089_00055"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEX6172372.1"
                     /translation="EAALDCGACGGNPGKANARVLAIMANKPLVREQLAKNGIAIPQD
                     TYFIAGQHNTTTDDIDLFDLEDLPPTHRKDLLQLVRDLELAGTRNSQERCARFPEIKV
                     SLSAAKARRMVYSRSADWSQVRPEWGLSGNAAFIIGRRALTRGINLDGRAFLHSYDYR
                     EDLDGRLLEVVMTGPQIVGQWINMEHYFSTVDPERYGSGSKIYHNVVGRFGIMSGAQS
                     DLRTGLAWQTVMDGVRPYHEPMRLVTLVEAPRERIDKIIGNQRHLKRLYDNQWLYLIA
                     IEPEAQLAYRYIPKEGWQAVKPNCSSRGQRFV"
BASE COUNT         1240 a         1572 c         1624 g         1344 t
ORIGIN      
        1 attttttttg agaattatgc tcctggcgtg gccgagcgcc tgggtcttgg ctatgaaaaa
       61 ctcagtgaga taaatccgcg attgatttac tgttcgctgt ccggttacgg gcagaacgga
      121 ccttaccgcg atgtgaaagc atacgatctg ttgattcaag gtgaaggcgg aatcattgcg
      181 accaccggct atcctgacaa acccgccaag gcaagtatcg ccatcgccga catcgcttct
      241 gggatgtacg cggttatcgg cattgtcctg gctctgtacc agcgcgataa aaccggtgag
      301 ggccaattga tcgatgtgtc gatgctcgac tcaatcgtct cgtggcttgg ttattttccg
      361 caccactact ggcatgcggg agaagagccg gggcgcgccg gcatgcggca ccattatgtg
      421 acgccgtacg ggccgtatct tgcagccgat ggcgagtatg tgaatcttgc tgttgccagt
      481 ccggccgatt gggagcagtt ttgccgagtg gttatagaaa agccggaatt gctggaagac
      541 tcccgcttta gaaccgtcca ggatcgacga aagaaccgcg ggtcgctgga agaaatcatc
      601 gaagcgattt ttctcgagcg cgatcacaag cattggctgg cgcagctcaa gaaagccgaa
      661 atgccgcatg gcatcgtccg cggcatcgct caggtgctgg cgcacccaca agtggcggcg
      721 cgaaaactga tccgcgaagc ggactcgccg gtgggtaaag ttccggtaat cgccaatgcg
      781 ctaaagatgt ccgatagccc tgcgcgatac gaccgcattc ccgaattggg cgaagacaat
      841 gacggaatcc tgcaagagct tggatacgac gcggcggcaa ttgcggatct gcggcgcgac
      901 aaggtgattt aaccgcttgt gttgcgacaa aaaggcgaag ccaaacagcc aacccactgc
      961 accacgaagg acacgaagct catgaagcaa aatagcctcg gatgaaagga tctcatgtgc
     1021 tttgtctcat ttggattggc ctctgcgaaa atgatcgctt ccttagcttc gtgtctttcg
     1081 tgtactcgtt cgaaaattgc ggctgaggtg aagagatgcg attccccaag atcgtcaccc
     1141 cgaatgtttt aatcgggggt ccagtccgga ttcccctgga ttcccgctag aagcatgcgg
     1201 gaatgacaga cttgttcgag actcagcggc ttggaggacg taccacattt tgattgtcta
     1261 tgggcgagtg taagctcatg accgattcgt ggtgacattt tttccgaacg gtgattcatg
     1321 accaaacccg tgattggcgt gatgggcgct tcagaaagcg acgcgctcac ggatgccgaa
     1381 aaagcccgcc ttaagcaact cgcggaaaaa ctcggcgctg agatcgccaa gcaccattgc
     1441 atcttgatct ccggagctac cacgggtctg ccggatctga tctcgcgatg cgcccgtgaa
     1501 cacggtggct ttgccgtcgg catctctccc gcggaaaacc gccgggagca catccaacgt
     1561 tacaaaatgc cgcccgatgc ggcggatctg cttatctata ccggcttcgg cctcaaggga
     1621 cgcaatgtcg tcaacgtgcg ctcggcggat atcgtcatca tcttcggcgg cgcaaccgga
     1681 actttgaatg aattcaccat cgcttacgac gaggggaaaa tcatcggcgt gctcgaaggt
     1741 agcggcggtt gcgccgatca cataaaggag attatcgaat tctgcaaaaa gccgacgcgg
     1801 gcgattgtgt tgtttgatag cgatccggaa aaactgatcg aggactgctt aaagtgttta
     1861 gacaaatgaa tggcaggcaa attagtcgag acaatcggtg aactccgtcg gtagaaacga
     1921 gccttgtccc tgcggcacga ttgccctgct gaaagatctt gaaagccgct cagagcgcca
     1981 acgccgtgca gggcaacccg cctatgactc catctggctg tggaaggagc tgggcatcat
     2041 aagagagtga gaagaggcgg cgcaaacggc ctcttccgtt cgattagaat tatctggttg
     2101 tccgtcgaat cagagtcgtg ccgacttctc gatgaacgct ttcacgatcg cattgaacgt
     2161 ctccggttgt tcccggtagc agaagtggcc ggcttcattg atgatgtgca tttccgattt
     2221 gggattgctg tctgcgatga tctcgaagag cgcgtggccg cgtttgagct cggcggtcgg
     2281 atcgttgtag ccccacacca acagcgttgg ctgcgtcagc ttcccgtgca tgatccagtt
     2341 gagcgtctct tccttgtgca gagctagatg tgtgaagaac tggtttattt taagcccctc
     2401 gtcctccatc tttctgacgg tttgttgata tctttccgat ttggcgatgt cttcacaagt
     2461 gtccagccag ttctgcgtga tgtgattcgc cctgaacgag tagtgctcga tgcaccagcg
     2521 ctggctgtcg cggctgaggc gcggtttggg tgcgttggcc atgatgaatt cggtcttcga
     2581 cggcccgggc gcaagcgtgc cgctgtcgac aatgacgcac gagcgaacca gttccgggtg
     2641 atccaaagtg agccgggtga cgacgtaacc gccgcgcgag tggccgatga cgtgaacctt
     2701 ttgcaatccg agcgtctgta atagacccgc cgcatgcttg accgttgccg ccatggtgta
     2761 gtccgcgtcg gatttcggat tgccggtata accctgaccg agcttgtcca tggcgatgac
     2821 ccgaaatgac tgcgcgagaa cgtcgaaatt aaactcccag tcgatggcgc tttcgcacgc
     2881 gtcgggtgtt cccatgtggc tgccgtgaac cagtaacagc acttcgccgc tgcccttatc
     2941 gaagtaacgc gttctgattc catcaacgtc gataaatttc gggtcggtca tcaaaacgtc
     3001 ctttcacccg taagatcacg gcacttgtcc ggacacgaac acggtatacc cccagacgct
     3061 cacggcgtta ttttttcagc gactccgcga gctcgcgtaa atacacttcc tgttccttgg
     3121 cctgatacag cgccatagag ccatcatctt tcaagggtat gccgccgatg aaatgaaaag
     3181 catgcgcgtc ccagtcgtag aattcgtcac cccgttcaca gatctctttc catttaagcc
     3241 cgaccggact gcggtggaga cccgtgcgcg ccggaagcgc ctcctgctgt tcgagggaca
     3301 ggttcgcatt tgccttgtct gcttcttctt ccggcagtcc gtcccacatg cgccggtagt
     3361 tgggaaaaaa ttcctgttcc cgcaccgtca gcgcggtgtg catgtcccac cacgggccat
     3421 gtccggcgac ccgctcgtcc accttgtcca ccagcttgta aatcagccgg gcgcccgcgc
     3481 gtaaacgaag caccagggta ccttcggaag ttgtggattt ctgatcagct tgtaggccgc
     3541 aatatttttc aaagaatgct gcggttctgg ccaggtcgcg gcaaccataa acagcatgac
     3601 tgatgcggcc gactctttcg tcggtggcga tctccatggc gcctaccggc atctgcgcgg
     3661 gcgcccagaa ttcatactga ttgccgtcgg gatcggcgaa ataaatgacc gtgccctggt
     3721 cgccttcggc acaggtttct aacggatcgc tgtgcggaat cttgtagcga tcgagccggc
     3781 gcaggtgcat gtcgagatct tcttgacgga tgtaaaagcc gcaacgcggt aactcttggc
     3841 caagttcctt tgccggcgga aagatctcgg gctgaagaaa cgcgccgatg cggcatgacc
     3901 cgccctccca ccagagaaag cagattacct tcatattcct tttgcgttcg gccgtggtca
     3961 gtcccctaaa cggtcttcgc gtaagcccga tgccgttcac cgcccagtca gcccaggcga
     4021 cgatatcgtt ggtgccgacg acaaaatgat cgatccaatg agccgcgcct ttgggttcga
     4081 ggttttccat ggaagagctc cttacaattt cgaaagttcc acaggtttta atccaatcct
     4141 gatgacatgt tcaagctatt gtaatcgatc atccaagact tggttcgtac tcctccgcag
     4201 cggcgggagg gaaaatatag aaattattga cggcccggat ccactaccgt cacgtactcg
     4261 cgaccgtacg aaaccgggaa aattctgtca agcgcgatgg ctagcagttc acgtgcttga
     4321 tgtttgattc gctaggcgtc cgagaaagtt ttgcagccgc gaaggcaata actgcaattg
     4381 cacgaagctt gcgtggatct taagctgcgc gcggttagcc ttcggttgaa gctgatggaa
     4441 gaagcactcg cctccaccgc aggaatgctc ttttcacttc atcgccggag tatcacaagg
     4501 atttacttct gagcggatcc tggggagaag tattctaccc ggctgacggc aacgtctgag
     4561 acgaacataa ctcccgtgta gcggtcgaac agaggtttca agccggccag aatcggctcg
     4621 actcgctccg gcgcaaccac tgacatcaac atgacgatgc tgtccatctc gttgaacatc
     4681 ggatgggcgg tgtgaaaacc gtggtgccct tttcccgaaa tattgtggat gatggtgtat
     4741 ccagtggcct tgttgtcgag caggtcggtg gcgaatttca agtgctcccc ctggatgata
     4801 atcctgatct ccttcatcgg atgaagcgtg agattgctca tgctgatctc cttttacacg
     4861 aatctttggc cacgagacga acagtttggt ttcacggcct gccaaccttc tttgggaatg
     4921 taccggtaag ctagctgtgc ttcaggctcg atggcgatga gatacaacca ttggttgtca
     4981 tacagccgct ttaggtggcg ttggttgccg atgattttat cgattctttc tcttggggcc
     5041 tcgaccaggg tcacgagccg cattggctca tgatagggcc gcactccgtc catgaccgtt
     5101 tgccaggcca ggccggtgcg gagatcgctc tgtgcaccgg acataatccc gaagcggccg
     5161 actacgttgt ggtaaatttt gctgccgctg ccgtagcgct ccgggtcgac tgtggaaaag
     5221 tagtgctcca tattgatcca ttggccgacg atttgaggtc cggtcatcac gacttcgagc
     5281 aaacggccgt cgagatcttc acggtaatcg taagaatgca gaaaagcccg cccatcgaga
     5341 ttgatgccgc gggttaaagc gcggcgccca ataataaacg cggcattacc ggacagtccc
     5401 cattccggcc gcacctggct ccagtcggcg ctccggctat acaccattcg tcgggctttc
     5461 gcagcgctca aactcacttt gatctcggga aaccgagcgc accgctcttg gctgttgcgc
     5521 gttcccgcca gttccagatc gcggacgagt tggagcagat ccttacggtg agtaggcggg
     5581 agatcttcca gatcgaacag atcgatgtca tcggtggtgg tattgtgttg tccggcgatg
     5641 aagtaagtgt cttgcggaat ggcaatgccg ttcttggcga gttgttcccg taccagcggt
     5701 ttgttcgcca tgatggctag gacccgagcg ttggccttgc ccgggttgcc accacaggcg
     5761 ccgcagtcga gagccgcctc
//
LOCUS       DASUTG010000003         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_741453, whole genome shotgun sequence.
ACCESSION   DASUTG010000003 DASUTG010000000
VERSION     DASUTG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741453"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF089_00060"
     CDS             complement(<1..356)
                     /locus_tag="VF089_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6172373.1"
                     /translation="MRRGVLARFIMFAVAALAAIVYLLPNLVDPLPSWWSSFLPSEKI
                     HLGLDLQGGTHLVLEVQVDKAVEHNIERVRNDLLNLFRERGLSGVSVERVQGTQLQVR
                     ATADSVERVRGVLKGD"
     gene            complement(359..694)
                     /gene="yajC"
                     /locus_tag="VF089_00065"
     CDS             complement(359..694)
                     /gene="yajC"
                     /locus_tag="VF089_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX6172374.1"
                     /translation="MFDFAFAQTAPGVGGPSQLISFLPLVLVFIIFYFLLIRPQQKKA
                     KEHQQMLGKLKKNDEVMTSGGIYGKVVALTDNIVTLEVAPNVRIRVNRPQISAVLTGE
                     KTIGKDAKE"
     gene            complement(723..1820)
                     /gene="tgt"
                     /locus_tag="VF089_00070"
     CDS             complement(723..1820)
                     /gene="tgt"
                     /locus_tag="VF089_00070"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX6172375.1"
                     /translation="MVNPFTLIKKDSGSEARLGRLETAHGVIETPCFMPVGTQGTVKA
                     VLPRDLKEMGCQILLGNTYHLHVRPGAELIQQLGGLHKFMGWDRPILTDSGGYQVFSL
                     GAMRKLSEEGVRFQSQLDGSYHMLTPEKVVRIQEALGSDIAMILDECIPHDAEREYVR
                     SSTERTVRWAERCLRARVSKDQLLFSIVQGGMHEDLRRWCVDKMRVMPFDGFAVGGLG
                     VGEGETTLQAISSYTAELLPQDRPRYLMGVGRPEDIINGIRAGFDMFDCVIPTRNARN
                     GTLFTSQGKVNIKRADFARDPRPLDELCECYTCRNFSRAYLRHLYMAGEILSSQLNSL
                     HNLYFYHRLMKRCREMIRDDRSDFDISSPAP"
     gene            complement(1951..>2279)
                     /locus_tag="VF089_00075"
     CDS             complement(1951..>2279)
                     /locus_tag="VF089_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX6172376.1"
                     /translation="EVAAKIQRAKDAGRRVVAVGSTTTRTLEWVALQKGKVEADEGIA
                     RLFIRPGDRFRVIDALITNFHLPQSTPLLIVIALAGLELIRKAYAEAIQKRYRFYSYG
                     DAMLIL"
BASE COUNT          568 a          663 c          544 g          504 t
ORIGIN      
        1 aaatctccct ttagaacacc gcgcacccgt tcgacactat ctgccgtcgc tcttacctgc
       61 agctgtgttc cttgaacacg ctcgaccgaa acacccgaaa gcccgcgttc acggaagaga
      121 ttaaggagat cattcctgac ccgttcgata ttatgctcaa ccgctttgtc aacttgcact
      181 tccaagacta aatgagtgcc gccttgcaag tccaatccca aatggatctt ttccgacggc
      241 agaaaactgc tccaccacga cggcaaggga tcaacaaggt tcggaagcag gtaaacgatc
      301 gcagccaaag ccgcaaccgc aaacataata aaacgagcca aaacaccgcg ccgcatggct
      361 actcctttgc atcctttcca atcgttttct cgccggtcag tacggcagaa atttgcggcc
      421 ggttgacgcg gatccgcacg ttcggtgcaa cctcaagagt tacgatatta tcggttaggg
      481 cgacaacttt accgtaaatt cctcccgagg tcatcacctc gtcgttcttt ttgagcttgc
      541 cgagcatctg ctgatgctcc ttcgcctttt tctgctgcgg cctaatcagc aagaaataaa
      601 aaatgatgaa gaccagcacc agcggcaaaa agctgatcaa ctggctcgga ccgccgacac
      661 ccggagcggt ttgggcaaat gcaaaatcaa acataagtcc tcctggaagt tctccttatt
      721 gatcagggtg ccgggctgct gatatcgaag tcactccggt cgtcgcgaat catttcacga
      781 caccttttca taaggcgatg gtaaaagtaa agattgtgca ggctatttag ctgagacgag
      841 aggatttccc ccgccatata gagatggcgc agataggcgc gtgaaaaatt acggcatgta
      901 tagcattcgc acaactcatc caacggtcgc ggatctcgcg cgaaatcggc gcgcttgatg
      961 ttgaccttgc cctgagacgt aaacagcgtc ccattgcgcg cgttgcgcgt cggaataaca
     1021 caatcgaaca tatcaaagcc tgcgcgtatg ccattgataa tatcttcggg cctgcccacg
     1081 cccatgagat agcgaggccg atcctggggc agcaactccg ccgtgtagga gctgatcgcc
     1141 tgtaatgtcg tctctccctc gccgacaccc aggccgccga cggcaaaacc gtcgaacggc
     1201 atcacccgca tcttgtcaac acaccatcga cggagatctt catgcatccc gccctggaca
     1261 atgctgaata gcaactgatc tttggaaact cgcgctctca agcaccgttc cgcccaacga
     1321 accgtccgct cggtagaact ccgtacatac tcgcgctccg cgtcatgcgg aatgcattcg
     1381 tccagaatca tggcgatatc gctgcccagc gcttcctgaa tccgtacgac cttttctggc
     1441 gtgagcatgt ggtaagaacc atcaagctgc gattggaagc gcacaccctc ttcagaaagc
     1501 ttgcgcatgg cgcctaagct aaatacttga tagccgccgc tgtcggtcag gatcggacga
     1561 tcccatccca taaatttgtg caagccgccc aattgctgaa tgagttccgc gcccggtcgt
     1621 acatggaggt gataggtatt gccgagcaat atttgacagc ccatctcctt tagatcccta
     1681 ggcaaaacgg ctttgacggt tccctgcgtt ccgaccggca tgaagcacgg cgtttcgatc
     1741 acgccatgag cagtttctaa acggcccagc cgagcttcgc ttcccgagtc cttcttaatc
     1801 agcgtaaatg gattgaccat ccttcaaatt cgatctgaaa tttcggattg tcgattgccg
     1861 caaaaaaaga tccaaacgaa gaagagatgc ttttcgcatg tacagtcttc tcccgaatcc
     1921 aaaatcgaaa accggcaagc taaaattctt ctaaagaatc agcatggcat cgccatagct
     1981 gtaaaatcga taccgcttct ggatggcttc ggcataggcc ttacggatca actccaagcc
     2041 agcaagagcg atgacgatta acaggggagt cgattgcggc aggtgaaaat tggtgatcaa
     2101 agcatcgatg actcggaacc tgtcgccggg acgtataaag agtcgcgcaa ttccctcatc
     2161 cgcctccacc ttgcctttct gcagtgcgac ccattcgagc gtgcgggtgg tggtcgagcc
     2221 gactgcgacc acacggcggc cagcatcctt ggcgcgctgg atcttagccg ccacttcag
//
LOCUS       DASUTG010000004         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1038053, whole genome shotgun sequence.
ACCESSION   DASUTG010000004 DASUTG010000000
VERSION     DASUTG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038053"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /gene="purH"
                     /locus_tag="VF089_00080"
     CDS             complement(<1..1100)
                     /gene="purH"
                     /locus_tag="VF089_00080"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6172377.1"
                     /translation="MGRIQRALISVSDKKGIVEFSRALSELGVELISTGGTASLLRDN
                     RVAVKDVSEITGFPEMMDGRVKTLHPKIHGGILALRDNPEHVAKMREHGIAPIDLVVV
                     NLYPFEATVARGGSFDEIVENIDIGGPSMVRAAAKNHRDVAVVVDPEDYPSIVAELQE
                     KGSLAKQTHFRLFTKAFQHTSHYDGAISNYFAALEENHKPTRWGETVNIQISKIQDMR
                     YGENPHQSAAFYGTQGDQGPSIARAKQFQGKELSFNNILDADAALSTVLEFSDTATVA
                     IKHNNPCGVALSKTSLADSFRKARACDPISIFGGVIAFNRPVDEETAGELKDIFLEIV
                     IAPSFTPAAKAVLSSAKRLLNIRLLELDMSEP"
     gene            complement(1115..>2094)
                     /gene="alr"
                     /locus_tag="VF089_00085"
     CDS             complement(1115..>2094)
                     /gene="alr"
                     /locus_tag="VF089_00085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6172378.1"
                     /translation="ARTLAAEGSDAFGVAILEEAIELRRHGITAPILVLTGVYLDQLD
                     LFFEHDLMPVVHDADLLQRLEAAVARRGRSLDVHLKIDTGMGRIGFPAAECDSWLPQI
                     KKLNALRVEGVFSHFSHAESVEGQYTRKQLEIFHNIVQRLAAVSIAPPVIHLANSAAT
                     ITLPEAYFNMVRPGLMLYGIYPSPEMADRISLKPVLSWKTRILQLKKVPSNASISYGQ
                     TFITQRDSLIATLPIGYADGYPRLLSNRGQALVGGKRAPVAGRVCMDLTMLDVTDIGK
                     IHQGDEVVLLGTQENQTISADEMASWADTISYEILTSIGARIPRIHHNS"
BASE COUNT          469 a          554 c          590 g          481 t
ORIGIN      
        1 tgtggctcgc tcatatccag ttccaaaagg cgaatgttga gcaatcgctt tgccgatgag
       61 agaaccgctt tagctgccgg cgtaaagctt ggagctatga cgatttccag gaagatgtcc
      121 ttgagttcac cggcagtctc ttcgtcgacc gggcggttaa acgcgatcac gccgccgaaa
      181 atcgatatcg gatcgcaggc tctggctttg cgaaaagaat cggccaacga ggtcttcgac
      241 aatgccacgc cgcaagggtt gttatgtttg atcgcgacgg ttgcggtgtc ggagaattcc
      301 agcaccgtac ttaatgcggc atcggcatct aaaatattgt tgaacgacag ttctttgcct
      361 tgaaactgtt tggcgcgggc aatcgaagga ccttgatcgc cctgagtccc atagaaggcg
      421 gcgctctggt gcggattttc gccgtagcgc atgtcctgga tcttggagat ttgaatattg
      481 accgtttctc cccaacgcgt cggtttgtgg ttctcctcaa gcgcggcaaa atagttggag
      541 atcgccccat cataatgcga ggtatgctgg aacgctttgg taaaaagccg gaaatgcgtt
      601 tgctttgcca gagagccttt ttcctgcaac tcggcaacaa tcgaagggta gtcttcggga
      661 tcgacgacca cagcgacgtc ccggtgattc ttcgccgccg ctcgcaccat gctcggtccg
      721 ccgatatcga tattctcgac gatctcgtcg aatgatccgc cgcgggcgac ggtggcttcg
      781 aatggataga gattcacgac caccagatcg attggtgcaa ttccgtgctc gcgcattttt
      841 gcgacatgct ccggattgtc tctcaatgcg aggattccgc cgtgaatctt gggatgaaga
      901 gtcttcactc gaccatccat catttcagga aatccggtga tctccgaaac atctttcacg
      961 gcaactctat tgtcgcgcag caacgatgcc gtgccgccgg tcgaaataag ctcgacgccg
     1021 agttcggaca aggcgcggga aaactcgacg atgcctttct tgtcagaaac gctaatcagg
     1081 gctctttgaa tcctgcccac gggtagtgat cctcctagga attgtgatgg atacggggaa
     1141 ttcgagcgcc gatggaagtg agaatttcgt aagagattgt gtctgcccag gaggccatct
     1201 cgtcggcgga aatggtctga ttttcctggg tgccgaggag aacaacctca tctccctggt
     1261 gtatttttcc tatatcggta acatccagca tagtcaaatc catgcacact cggccggcca
     1321 ccggcgcacg cttgccgcca accaatgcct ggccacgatt ggaaaggagc cggggataac
     1381 catcggcata accaatcggc agggtcgcga tcaagctgtc acgttgggta atgaacgtct
     1441 gaccataact gatgctggca ttggacggga ccttcttgag ttgcaagatg cgcgttttcc
     1501 atgacagcac cggtttcagc gaaattcgat cggccatctc aggtgacggg taaattccat
     1561 agagcataag acccgggcgc accatattga agtaggcctc cggcaatgta atcgtcgccg
     1621 cgctgttggc cagatgaatc accggcggcg caatgcttac ggcggcgaga cgttgcacga
     1681 tgttatgaaa tatctccagc tgcttgcggg tatattggcc ttcgacgctt tcggcgtggg
     1741 agaaatgcga aaagactccc tcgactctga gcgcgttgag cttttttatc tgaggcagcc
     1801 atgagtcgca ctcggcggcg ggaaatccga tccgacccat gccggtatcg attttgagat
     1861 gaacgtccag acttcgacca cggcgggcaa cggccgcctc caggcgctgc agaaggtcgg
     1921 catcgtgcac caccggcatt aggtcatgct cgaagaacaa atccagctgg tcgagataaa
     1981 caccggtgag cacaaggatg ggggcagtga tgccatggcg tcgcagctca atggcttctt
     2041 cgaggatggc aacgccgaag gcatcgctac cttcagcggc gagagttctg gcca
//
LOCUS       DASUTG010000005         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1927457, whole genome shotgun sequence.
ACCESSION   DASUTG010000005 DASUTG010000000
VERSION     DASUTG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927457"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VF089_00090"
     CDS             <1..952
                     /locus_tag="VF089_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6172379.1"
                     /translation="SESDPTLLTLYGTVDLRQVQLSFNNSERIAAVLVQEGDRVRQGQ
                     LLARLDTARLEPQLAEAEAQAAAQLQVVKRLRSGSRPEEIAQARANLQAAQADLANAR
                     QQYERWKSAAEISAGRAVRQQDVDNARTAVQVAEARLAVVQKTLDLAIAGPRLEEINE
                     NEARLKAFEAHANVLRQQLKDAQLISPMDAVIRTRVMEPGEMASPQRPVFSLAITDPK
                     WVRAFISEPDLGKIKPGMPAAVVVDSFADRRFEGWIGFISPVAEFTPKTVQTEELRTS
                     LVYEVRVFVKDPADELRLGMPASVYLQLKGRELEGRDERP"
     gene            939..2861
                     /locus_tag="VF089_00095"
     CDS             939..2861
                     /locus_tag="VF089_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6172380.1"
                     /translation="MNDHDSSNVLIGRDIRKTFRRDSGERVDALNGVSIDVAKGTLTA
                     LVGPDGAGKTTLIRLAAGLMLPDSGDLKVIGIDVAAEPQRVQDRIGYMPQRFGLYEDL
                     SVQENLELYADLHGVSSEQRRQRYPSLMEMTALGPFTERLAGRLSGGMKQKLGLACTL
                     VRAPELLLLDEPTVGVDPLSRRELWNIILQLVNQQNMTVLLSTSYLDEAERCSHVAVM
                     HEGNILAQGHPHGISSLAQRRVFLAQPPAGQKAREYQACLLDKREVVDAVPQGGRVRI
                     VTDESGKLQETERIGQREDKETDRQGDSDDLNAIAVSVEPRFEDGFMVLLKSKKGSGR
                     ETDGRKRLGEKEISRHSEADRQKTDSETRSYANKQVSPVLPVSRSSDGAMVQVQNLVR
                     RFGKFVAVDHVTFEVQRGEIFGLLGPNGAGKTTTFRMLCGLLPASEGTLRVAGADLRR
                     ARASARQHIGYVAQKFSLYGQLSVSENLDFFARAYSLRGTEKSRRIDWALQQFGLSAL
                     TNLPSGNLPGGFKQRLAMAAALLHEPETLFLDEPTSGADPLARRDFWRRITALSQGGV
                     TVVITTHFMEEAEYCDRIAILDAGRILAQGTPAAIRGLAIGEPGSEPTMEDAFIAIVE
                     QARAKQAQTADARGAA"
     gene            2858..>3076
                     /locus_tag="VF089_00100"
     CDS             2858..>3076
                     /locus_tag="VF089_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172381.1"
                     /translation="MNASFNSRRISALVRKEAKQMLRDPSSIAIGIIMPVLLILLFGY
                     GLSLDVTRVPVAVVLEDPSPAATELAAGF"
BASE COUNT          702 a          805 c          956 g          613 t
ORIGIN      
        1 aagcgagagt gatcccacgc ttctgacgct ttacggcact gtcgatctcc ggcaagtaca
       61 gctttctttc aacaacagcg agcgtatcgc agctgttttg gttcaggagg gcgatcgtgt
      121 caggcagggt cagttgctgg cacgcctcga taccgcaaga ctggagccgc agctggctga
      181 ggccgaggcg caagccgcag cgcagctgca ggttgtgaag cgactgcgca gcggcagtcg
      241 ccctgaagag atcgcccaag ctcgggcgaa tctgcaagca gcgcaggctg atctggccaa
      301 tgcgcggcaa cagtatgagc gttggaaaag cgccgccgaa atttctgccg gccgggcagt
      361 gcgtcagcag gacgtggaca atgcaagaac tgcggtgcaa gtcgcggaag ccagattagc
      421 ggtagtccag aaaacattgg acttggcgat agccgggcca cgcctagaag aaataaacga
      481 gaacgaagcg cggctgaaag cattcgaggc gcatgccaat gtgctgcgcc agcaactgaa
      541 agacgcccaa ctgatctccc ctatggatgc ggtaatccgc acccgagtga tggagccagg
      601 agagatggcg tcgccgcagc ggccggtgtt ttcgctggcg attaccgatc caaagtgggt
      661 ccgtgccttc atctctgagc cagatcttgg aaagataaaa cccggcatgc cggcggctgt
      721 tgtagtcgat agctttgcgg atcgccgttt cgaagggtgg atcggattca tttcgccggt
      781 agccgaattt acgccaaaaa cagtgcagac cgaggaattg cgcaccagcc tcgtctacga
      841 ggttcgtgtg tttgtcaaag atcccgcgga tgaacttcga ttagggatgc cggcgagcgt
      901 ttacttgcag ttgaagggca gagagctcga gggtcgcgat gaacgaccat gacagctcga
      961 atgtcttgat cggccgcgat atccgcaaga cattccgccg cgacagcggt gaaagagtcg
     1021 atgcactgaa cggcgtatcc atcgatgtgg ctaaagggac gttgacggcg ctggtgggac
     1081 ccgatggggc aggaaaaact acgctgatcc gtcttgctgc cggtttgatg ctgccagaca
     1141 gtggcgatct taaagtcatt ggcatcgatg tcgctgccga gccgcaacgg gtgcaggacc
     1201 ggattggcta tatgccgcaa aggttcggct tgtacgaaga tttgagcgtc caggaaaatc
     1261 tcgagttgta cgccgactta catggcgtct cctcggaaca acggcgccag cgctacccga
     1321 gtttaatgga gatgaccgcg ctggggcctt tcaccgagcg attagccgga cggctctcag
     1381 gcggcatgaa gcagaaattg gggctggcct gcacactggt gcgcgcgccg gagttactgt
     1441 tgctggacga accgacggta ggtgtggatc cgctttcgcg gcgtgagctg tggaacatca
     1501 ttctacagct ggtcaaccag caaaatatga cggtattgct gagcacctcc tatttggacg
     1561 aggccgaacg ctgcagtcat gtcgcagtga tgcacgaagg taacattctg gcgcaaggtc
     1621 atccgcatgg catcagttca ttagctcaac gccgggtatt tcttgcccaa ccgccggcgg
     1681 gccaaaaggc cagagagtat caagcttgct tattagacaa gcgggaagtg gtggatgccg
     1741 tcccccaagg cgggcgggtg cggatcgtta cggatgaaag cggaaagctg caagaaaccg
     1801 agcgcattgg acaaagggaa gacaaggaga ccgacagaca aggagactca gatgacttaa
     1861 acgccatagc tgtttcagtg gagcctcgct tcgaagatgg atttatggtg ctgctcaaat
     1921 caaagaaggg gagtggaaga gaaacggacg gtcgaaagag actgggagag aaggagattt
     1981 cgagacattc agaggcggac cgacagaaaa cagactcgga gacaaggagc tatgcgaaca
     2041 aacaggtctc tcccgttctc ccggtctctc gatcttccga tggggccatg gttcaggttc
     2101 aaaatctggt tcgccgcttc ggaaaatttg ttgccgttga tcatgtcacg ttcgaagttc
     2161 agcgcggcga aatcttcgga ctgctgggtc caaatggggc gggcaagacc acgacctttc
     2221 gcatgctctg cggtttattg cccgcaagtg agggaacgtt gcgagtggct ggagcggatc
     2281 tgcgccgtgc gcgcgcctcg gcgcgccagc acatcggcta tgtcgcgcag aagttttcac
     2341 tttacggcca gctctcagtc agcgaaaatt tggatttttt tgccagggct tacagcctgc
     2401 gcggcacgga gaaaagtcgc cgtatcgatt gggctctaca acagttcggg ctctcagcct
     2461 tgacaaatct gccaagcggc aacctccccg gcggttttaa gcagcgcttg gcgatggcgg
     2521 cggcgctgct gcatgagccc gaaacgcttt ttcttgacga gcctaccagc ggcgccgatc
     2581 ctttggcccg tcgcgatttc tggcggcgca tcaccgcgct ctcccaagga ggagtgacgg
     2641 tcgtgatcac gacccatttc atggaagaag ccgaatactg cgatcgcatc gcgattctag
     2701 atgccggcag aatactcgcg cagggtacgc ccgcggcaat tcgcggccta gcgattggag
     2761 aacccggaag cgaaccaacg atggaggatg cctttatagc gatcgtcgaa caggcgcgcg
     2821 caaaacaggc gcaaaccgcc gacgcgcggg gtgcggcatg aacgcaagtt ttaattcgcg
     2881 gcgcatcagt gccttggtgc gcaaagaggc gaaacagatg ctgcgcgatc caagtagcat
     2941 tgccattggc atcatcatgc cggttctgct cattttgctg tttggctatg gactatctct
     3001 cgatgtaacc agagtgccgg tggcggttgt cctggaagat ccatcgcccg ccgctacgga
     3061 attagccgcc ggattt
//
LOCUS       DASUTG010000006         8186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_445681, whole genome shotgun sequence.
ACCESSION   DASUTG010000006 DASUTG010000000
VERSION     DASUTG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445681"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF089_00105"
     CDS             <1..656
                     /locus_tag="VF089_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6172382.1"
                     /translation="ARTGAAGGIGAKYLARNDSQTAAVLGSQAHAGMQLQAAHAVRSL
                     TSATVFSPTESHRSACAEKIRRKTGIEARAVSSASEAIEDADIIITATDSRTPVFDGN
                     LLKPGVHITSMSNGDKARTRQEIDEATIRRAKPIFITSKETVCVNESDIFRAVRDKII
                     SWDSVHEISDLLLNRIPGRTDDRQITLYKLQGTGIMDVAIGALAYERLKGSNLTQTL"
     gene            683..2089
                     /gene="sthA"
                     /locus_tag="VF089_00110"
     CDS             683..2089
                     /gene="sthA"
                     /locus_tag="VF089_00110"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX6172383.1"
                     /translation="MARDDMLVIGSGPAGQKAAIQGTKVGKKVGIVERKRFVGGICIN
                     SGTIPSKSLREAVLFLSGFRQRNLYGASYRVKKDITFEDLLFKCDHVVKAEQEVVQNQ
                     LIRNHVDLIVGNASFIEPHRLSIAQDLETNEHSADYIMIAVGTEPSRPVGIPFDGTTI
                     IDSDGLLALNQLPKSLTIVGGGVIGCEYASILATLGIPVVLIERRPRLLEFVDSELIE
                     ALQYQLRSIGVTLRFNEEVTAVEKESDGPVLIQLKSGKHIRAALLMYSLGRVGATKGL
                     NLESIGIRSDERGRLIVNENFQTTIPHIYAVGDVIGFPALASTSMQQGRHAACHALGL
                     ACDTSTHLLPYGIYTIPEISMVGRNEDELTRDGVPYEIGVARYREIARGELIGDTVGM
                     LKLLFHSDTRELLGVHIIGEGATELVHIGQAVMAFRGKLDYFVDAVFNYPTLAECYKV
                     AALAALNRFPAIACSPAV"
     gene            complement(2126..2524)
                     /locus_tag="VF089_00115"
     CDS             complement(2126..2524)
                     /locus_tag="VF089_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6172384.1"
                     /translation="MADNIPEPFKDLFAKVAYANLATVMPDGSPQVTPVWFDYDGECL
                     RINSAKGRIKDKNMRRNKRVAVSIQDPDNAYRYLALRGNVEEITEEGADTHIDSLAKK
                     YLDKDKYPFRGPGEVRVIYKIRPEKVSTMG"
     gene            complement(2551..3198)
                     /locus_tag="VF089_00120"
     CDS             complement(2551..3198)
                     /locus_tag="VF089_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6172385.1"
                     /translation="MKRPIDLIMFDLDGTLADTGPDLADSVNYTRSRFELTPLPQPLI
                     YTHIGRGVEYLLRHAVAEKAPRHFSEIMRVFLEHYQQHLLDKTVLYPHVTEVLDYFGD
                     KRNAVVTNKLHHLAVTVLRGLGIEDRFDAVLGGDSGAEKKPDPALLRLVLRRFQIIPA
                     NALMVGDGDTDIEAGRCAGVITCGVTYGLANRGDLVAAKPDFMIDDLAQLTLDFC"
     gene            3324..4319
                     /locus_tag="VF089_00125"
     CDS             3324..4319
                     /locus_tag="VF089_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172386.1"
                     /translation="MNVIRLAKTLLWVGLVLFIMPVYAAAQKINTSYISTTPGSSSVM
                     WVAKDAKIFEKHGFDATVIFISGSVRGIQAILAGEIPIGEGGGPGLASARLAGGDVVA
                     IAGNVNVLPYYLVSDPSIKKAEDLKGKIGGTHIAGTTAEFALKVGLKRIGLEPAKDVS
                     LRVIGGAIDRMVALQKGIVQFTVVTEAGKAIAEKLGYPTVVDMAALQIPFPQNGVYTS
                     TKLIRDNPDMVRRYMRAYTEAIHFFKTRKEDTIKIMSKYSRLEDRKTLNEAWDWHAKF
                     IPEAPYPPVEGYQLILQDMAATNPKAAQANAKDLVDARFVKELEDSGFIKNLYKK"
     gene            complement(4484..5563)
                     /locus_tag="VF089_00130"
     CDS             complement(4484..5563)
                     /locus_tag="VF089_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6172387.1"
                     /translation="MISIRKLRKTFPAQQGVVNALRDIDLEVTEGDFCVLLGPSGCGK
                     TTTLRCVAGLERPDGGEIEISGRLVNSPGSRIFVPAEKRQIGMVFQSYAIWPHMNVFQ
                     NVAFPLTQGQKRVSTSMVKERVRAALQRVQLDGLEDRPATDLSGGQQQRVAMARAMVT
                     DSKVLLMDEPLSNLDARLREQMRVELRKITKSIGVTTLYVTHDQAEALSLGDKVCVMN
                     HGEILQIARPNEVYARPVNLFVAQFVGEMNFVKAEVIESGQVGCSLGKLPISIPSGVS
                     KGANITLAIRPEHVKVFPLTNNNANHVCGKITSKNYLGDSALLEVEVNGTTLLAKLDG
                     DTELSMGDSAAVEFPTHRWHVFPQN"
     gene            complement(5758..7515)
                     /locus_tag="VF089_00135"
     CDS             complement(5758..7515)
                     /locus_tag="VF089_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6172388.1"
                     /translation="MKVESHSESVGVWEGIGRRMPQKVSLFAGGLAGFLSILIGLPVV
                     MVVLMSLRTGFPGEDVPFTLENYAYVYFTPRTYEVLLNTFFFAVSSVFITLLIAVPLV
                     WILMRTDIPFKKTIYVLLTIGILIPVFLRTIAWILLLSPRIGLLNKWLQELFALSNPP
                     LNLYSITGMAFVQGVSFVPAAFFMLAAAYRSMDPSLEEAAYTSGVGKLKTFLKINIPI
                     TWPAIAAVMVYLFMTAIAVFEVPAIIGLPARIHVLSSLIFTSTTPSTGLPEYGMAGAY
                     GAIMLVIGLALAFLYVRLVKQGKKYTVITGRGYRPREIALGKWKWAALGFVFFYLAIE
                     VFIPFAVLLWASLLPYLQLPSAEAVSSLTLKHYIELPTHVGPRPFINTLILMVAVPTV
                     TMFLSVLVSWVVIRTQVSFRGFLDTLAFIPHAVPGILMAVGLAYLGLAYRDYFPLYGT
                     VAIIVLAHTINWIAYGTRTTNSVMIQVHRELEEAGRVSGASQPRVLGKIILPLIAAGV
                     LNSWIWIGMLSYREVTMSLTLLTRNNIVISTVVWQFWGSGWIPQVSALGVILILFATI
                     VVGTLRFALSRIGDIGHAS"
     gene            complement(7554..>8186)
                     /locus_tag="VF089_00140"
     CDS             complement(7554..>8186)
                     /locus_tag="VF089_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6172389.1"
                     /translation="EYDRGTVSVANQFALPTYNSKLVAAKDVPKKWEDLLDPKWKGKL
                     GVINSTHHWGRLAAGPWGEEKTLDFVKKLSAQKPLLTRAGEMAQRLILGEVLVSATLQ
                     DSQLHESQQSGAPLAFAEEVSPVISPEYHVGVLKNAAHPNVGHLFVIFMASPEVQQIW
                     KKHTGHSSAYVPGTDAYKFVQGKKVVYMKQDQAEKVDKISRQIGKILGFN"
BASE COUNT         1952 a         2204 c         2201 g         1829 t
ORIGIN      
        1 aagcccgtac cggagcggcg ggcgggatcg gcgccaaata cctggctcgc aacgactcgc
       61 agactgcggc ggttctgggg agccaggctc atgcggggat gcagcttcaa gcagcgcacg
      121 cggtcagatc tttgacatcg gcgacggttt ttagcccgac ggaatctcat cgcagcgcct
      181 gcgccgagaa aataaggcga aagactggga ttgaagctcg tgcggtctca agcgcttcag
      241 aagccatcga ggatgccgat atcattatca ccgcgacgga ttcccgaact ccggtgttcg
      301 acggtaacct gctaaaaccc ggcgtgcaca ttacctccat gtccaatggc gataaagctc
      361 gcacgcgcca ggaaattgac gaagcgacga tccgccgggc caagccgatc ttcattactt
      421 cgaaagaaac cgtttgcgtc aatgaaagcg acattttccg cgctgtgcgc gacaaaataa
      481 tttcctggga ttccgtgcac gaaatttcag atctgctgtt gaaccggatt ccgggccgca
      541 cggatgaccg gcaaatcact ctgtataagc tgcaaggaac aggtatcatg gacgtcgcca
      601 tcggcgcgct cgcgtatgaa agactcaaag gaagcaatct cacgcagaca ctctgaggcg
      661 gcgcgatgga tggaaggcga taatggctcg cgacgacatg ctggtgatcg gctccggacc
      721 cgcaggtcaa aaggcggcga tccaagggac taaagtcggc aagaaggtcg gcattgtcga
      781 acgaaagaga ttcgtgggtg gtatctgcat caattccggc actatcccga gtaagtctct
      841 gcgcgaagcc gtgctgtttc tttcaggctt taggcagaga aatttgtacg gtgccagtta
      901 tcgcgtcaaa aaagacatca ctttcgaaga cctgcttttt aagtgcgatc acgtcgtcaa
      961 agcggagcag gaagtggttc aaaatcagct catccgtaat cacgtcgatt tgatcgtcgg
     1021 taacgcaagc ttcatcgagc cgcataggct ctcgattgca caagatctgg aaacgaacga
     1081 gcatagcgcg gactacatta tgattgctgt gggcacggag ccgtcgcgcc ccgtggggat
     1141 tcccttcgat ggtaccacaa tcatcgatag tgacgggttg ctggcgttga atcaattgcc
     1201 gaagtcgctc acgatcgtag gtggcggcgt catcggttgt gaatacgctt cgattctcgc
     1261 caccctgggg attccggtgg tcttgattga acgccgcccg cgtttacttg aatttgtcga
     1321 cagcgaatta atcgaggcgc ttcagtatca gttgcgtagc atcggcgtca cactgcgctt
     1381 taacgaagaa gtcaccgccg tagaaaagga gtctgacggg ccggtgctaa tccagctcaa
     1441 gagcggcaaa cacattcgcg cggccttgtt gatgtattcg ttaggccggg taggcgctac
     1501 aaaaggattg aatttggaaa gtatcggcat ccgatcggat gagcggggac ggcttattgt
     1561 caatgagaac tttcagacga cgatcccgca catctatgcg gttggcgatg tgattggatt
     1621 tcccgcgttg gcttccacct ccatgcaaca gggtcggcac gccgcttgtc acgcattggg
     1681 tcttgcttgc gacacctcga cccacctgct cccttacgga atctacacga ttccggagat
     1741 ctcaatggta ggacgcaatg aagatgagct cacccgtgat ggagtgccat acgagatcgg
     1801 tgtcgcgcgt tatcgtgaga tcgctcgcgg tgagttgatc ggagataccg tgggcatgtt
     1861 gaaactgctg tttcatagcg atacccgcga gctcctcgga gttcacatca tcggcgaggg
     1921 tgcaacggaa ttggttcata tcgggcaggc tgtgatggcc ttccgcggca agctggatta
     1981 cttcgtggat gccgtgttta actacccgac gctcgccgaa tgctacaaag tagccgcgct
     2041 cgcggcgttg aaccgctttc cggcaattgc gtgctcgcct gcggtttgag cttgatgaac
     2101 tcgcgcttcg aaagcttcta ccgggctaac ccatggtaga aaccttctcg ggacgtatct
     2161 tatagattac ccggacttcg ccggggccgc gaaacgggta tttgtccttg tctaaatact
     2221 ttttggccaa ggaatcgata tgagtgtcgg cgccttcttc ggtgatttcc tccacgttcc
     2281 cgcgcaacgc tagatagcga taggcgttgt cgggatcttg aatcgagaca gcaacccttt
     2341 tgttgcgacg catgtttttg tctttgattc tacccttggc tgaattgatg cgcaggcact
     2401 cgccgtcata atcgaaccac acaggcgtta cctgcgggct gccgtcgggc ataacggtgg
     2461 caagatttgc ataggcgact tttgcgaata gatctttgaa gggttccggt atgttatctg
     2521 ccatgatctg cctccttgtg cgtccctttg ttaacagaaa tctagtgtca attgcgccag
     2581 atcgtcgatc ataaagtcag gtttggcggc aaccaaatcg cctctattgg ccagtccata
     2641 ggtcacgccg caggtgatca cccccgcgca tcttccggct tcgatgtcgg tgtcaccatc
     2701 gccgaccatc agcgcattgg ctgggatgat ttgaaaccgc ctcagcacca accgcagcag
     2761 cgcggggtcc ggttttttct cggcgccgct gtcaccgccc aacaccgcgt cgaagcgatc
     2821 ttcgatacct agcccgcgca gcactgtcac tgccagatga tgcaatttgt tggttaccac
     2881 ggcgttgcgt ttatcgccga aataatccag cacttcagtg acgtggggat acagcacggt
     2941 cttatcgagc aagtgttgct gatagtgttc aaggaatact cgcataattt ccgagaagtg
     3001 ccgcggcgcc ttctccgcga cggcatgcct cagaagatac tcaacaccgc gcccgatatg
     3061 agtgtagatc aacggctgcg gcaatggggt cagttcaaaa cgcgagcgag tgtagttaac
     3121 cgaatcggca agatccggtc ccgtgtcagc cagtgttcca tccagatcga acatgatcaa
     3181 atcaatgggt cgcttcatcg aagttgtttc tctgattcaa aagtaaaccg taaaccctaa
     3241 ccctgtcaat tcagcttttc agcgctcctc gaagatgata ctagtggcaa tatttcgttc
     3301 tcatcgaagc ttccggaggt aaaatgaacg tgatccgatt ggcgaaaaca ctgctctggg
     3361 tcggcctagt ccttttcatt atgccggttt acgccgcggc ccaaaagatt aacacatcgt
     3421 atatttcgac cacgcccggt tcttcttcgg ttatgtgggt tgcgaaagac gcgaagatct
     3481 ttgagaaaca cggcttcgat gcaaccgtaa tttttatttc aggcagcgtt cgcggtatcc
     3541 aagccatctt ggcgggggag attcctatcg gcgaaggtgg cgggccgggc ttggccagcg
     3601 cgcgtctagc cgggggcgat gtcgttgcca tagccggcaa tgtcaatgtc ttaccttact
     3661 atctggtctc agatccgagc atcaagaaag ccgaagatct caaaggaaag atcggcggca
     3721 ctcatatagc gggaaccact gctgagttcg cactcaaagt aggtctaaaa agaatcggct
     3781 tggaaccggc taaagatgtg agcctccggg ttatcggtgg cgccatcgat cgtatggtcg
     3841 ctctgcagaa gggaatcgtg caattcaccg ttgtcactga ggcaggaaaa gccatcgccg
     3901 agaaactcgg ctatcctacg gtggtggaca tggcggccct gcagataccc tttccgcaaa
     3961 acggtgtcta tacctcgacg aaactgattc gtgataaccc ggacatggta cggcgttaca
     4021 tgcgcgccta taccgaagcg atccactttt tcaaaacccg caaggaagac acgattaaaa
     4081 tcatgagcaa atattctcga ttggaagacc gaaaaacctt gaacgaagct tgggactggc
     4141 acgcgaagtt cattcccgaa gcgccctatc cgccggtgga agggtatcaa ctgattttgc
     4201 aggatatggc cgcgactaat ccgaaagccg cgcaagccaa tgctaaagat ctggtagatg
     4261 cacgtttcgt caaagaactg gaagactccg gatttatcaa gaacctgtat aaaaaatgaa
     4321 gatcggaaag ttcaacagtt ccaccgttca acgtcagatc tcgcgtaact agcccgatca
     4381 attacctgga gatttaaccc tcacgagtag cgacggaacg cttggaattg cttggacgct
     4441 tgagcagatc ttaccttagg acgtggaact gttgaacgtt gatttaattt tgtggaaaga
     4501 catgccatcg atgcgtcggg aattccacgg cagccgagtc acccatggat aattccgtgt
     4561 cgccatcgag ctttgcgagc aacgtcgtgc cgttaacttc cacttccagc agcgccgagt
     4621 cgcctagata gtttttgctg gtaatttttc cacagacatg attggcatta ttgtttgtca
     4681 gcgggaaaac tttgacatgc tcaggccgga tcgccagagt gatgttggcg ccctttgaca
     4741 cgccactagg aatcgagatg ggtagtttac ctaatgagca gccaacctgt ccggattcaa
     4801 ttacttccgc tttgacaaag ttcatctcac cgacaaactg cgcgacgaac aaattcaccg
     4861 gccgagcata gacttcgttg ggccgggcga tctgcaggat ctcgccgtga ttcatgacgc
     4921 aaactttatc gcccaggctc aaagcttcgg cttgatcatg ggtgacgtag agagttgtca
     4981 cgccaattga ttttgtaatc ttgcgcaatt ccacgcgcat ttgctcgcgc agccgcgcgt
     5041 ctagattgct gaggggctca tccatcaaca gcaccttgga atccgttacc atggcgcgcg
     5101 ccattgcgac gcgctgttgc tggccgccgc tgaggtcggt agccggccgg tcttccagtc
     5161 catcgagttg aactcgttgc agagccgcgc gcacgcgctc cttaaccatg gaggtcgaga
     5221 ctcgcttctg accttgagtc agcggaaacg cgacgttttg aaacacgttc atgtgcggcc
     5281 agatagcgta ggactgaaag accatgccga tctggcgctt ttcggccgga acaaagatcc
     5341 gcgaaccggg agaattgacc agccgcccgc tgatttctat ttcgccgccg tcgggcctct
     5401 caagtccggc gacgcatctg agggtagtgg ttttaccgca gccgctcgga cccagaagca
     5461 cgcaaaaatc gccctccgtc acttccagat cgatatcccg cagcgcattg acaacgccct
     5521 gttgcgccgg gaaggtttta cgaagctttc ttatgctaat catctttcct cactgacaga
     5581 ccgatgcaca agctcgagct gttcgtttgc actcgatcgc tggcaatgac gaccttaatg
     5641 tacatccgcg cttgttggct gctggcctcc gggcatcgaa atcttttgac ccccctaatc
     5701 gtctattttg atccgtccga gctggaatcg ttttcagttg cccgccgggc acatacacta
     5761 ggaagcgtgg ccgatatcgc caatgcgcga gagcgcaaag cgcaaggtgc cgacgacaat
     5821 ggttgcaaac agtatcagaa ttactcccaa cgccgacacc tgagggatcc acccactccc
     5881 ccagaactgc cagacgactg tggaaattac gatattgtta cgggtgagaa gggtcaacga
     5941 catggtgacc tctcgatacg acagcatgcc gatccagatc cagctgttga gaactccggc
     6001 cgcgatgagc ggcaaaatga tttttcccaa aacgcgcggc tgcgaagctc cggagaccct
     6061 gcccgcctcc tccaattcac gatggacctg aatcatgacg ctgtttgtcg ttcgcgtgcc
     6121 gtaggctatc caattgatgg tatgcgcgag tacgataatg gcgaccgtgc cgtatagcgg
     6181 aaagtaatcc cggtaagcca accccaggta cgctaacccc actgccataa gaattcccgg
     6241 caccgcgtgc ggaataaaag cgagcgtatc gagaaaaccg cggaacgaga cctgagttct
     6301 aatcacaacc caggatacca gcacgcttaa aaacatggtt accgtaggca cggccaccat
     6361 gaggatgaga gtattgataa agggccgggg gccgacgtga gtaggaagct cgatatagtg
     6421 tttaagcgtt aaggatgata ccgcttccgc tgacggcagt tgtagataag ggagcagcga
     6481 agcccacaac aaaacggcaa acggaataaa aacctcaatc gccagataga agaagacgaa
     6541 accgagagcc gcccatttcc atttgccgag cgcgatttcc cggggtcgat agcctcgtcc
     6601 tgtgatgacc gtgtattttt tcccttgttt caccaagcga acatacagaa aggccagcgc
     6661 cagcccgatt acgagcatga tcgcgccgta agcgccggcc atgccatact cgggcaaccc
     6721 ggtcgacggt gtcgtggaag taaaaataag cgaactcaaa acgtgaatac gcgccggcag
     6781 cccgataatc gccgggactt cgaagactgc aatcgccgtc atgaacaaat acaccataac
     6841 cgcagcgata gccggccagg taatcggaat attgatcttc aaaaatgttt taagcttgcc
     6901 gacccccgaa gtataggcgg cctcttccaa ggacggatcc atggaacggt aagccgcggc
     6961 cagcataaaa aaagccgcgg gtacgaaaga gacgccttgc acgaacgcca tgccggtgat
     7021 gctgtacaaa ttcaaaggtg gattgctcag ggcaaacaac tcttgcagcc atttgttcaa
     7081 caaaccgatc cgcggactga gcagcagaat ccatgcgatc gtccgcagaa ataccgggat
     7141 gagaataccg atagtcagta aaacgtaaat ggtcttcttg aacgggatgt cggtcctcat
     7201 caggatccaa accagcggaa cagcgatcag aagagtgatg aagacgctgg aaactgcaaa
     7261 gaaaaacgtg ttgagcagga cttcataggt ccgcggtgtg aagtaaacgt aggcgtaatt
     7321 ctccagcgtg aacggaacat cttcacctgg gaagccggtg cgaaggctca tgaggacgac
     7381 catcacaacc ggcaacccga tcaatatgct taggaatccg gcaagacctc cggcgaacag
     7441 ggataccttc tggggcatgc gacggccgat gccttcccag acgcccacgg attcgctgtg
     7501 cgattctacc ttcatgtcag gctccctgtg cccttagcgg cacgtggacg ttttcagttg
     7561 aaaccgagga tcttgccgat ctgccgcgag atcttgtcaa ccttttcggc ctggtcctgt
     7621 ttcatgtaga ccaccttttt gccttgaaca aacttgtagg cgtccgttcc cggaacgtat
     7681 gcggacgagt gaccggtatg cttcttccaa atctgttgca cttccggcga agccataaat
     7741 atcacgaaca gatgaccgac atttgggtgc gccgcgttct tcagtacgcc gacgtgatac
     7801 tcgggcgaga tgacgggcga aacctcctcg gcgaaagcca gaggcgcgcc ggactgctgc
     7861 gattcgtgca gctggctgtc ctgtagagtc gccgacacca gcacttcgcc caaaatcaac
     7921 cgctgcgcca tttctcccgc ccgagtcaaa agaggttttt gcgcggacag tttcttcaca
     7981 aagtcgagag tcttctcttc gccccatggg cctgcggcga gccggcccca gtggtgggtg
     8041 gagttaatga cgccaagttt gcccttccat ttcggatcga gaagatcttc ccacttcttt
     8101 ggcacatcct tggccgcaac cagtttgctg ttgtaggttg gcagggcaaa ctggttggcg
     8161 acagaaacgg tgcccctgtc gtactc
//
LOCUS       DASUTG010000007         6778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_297480, whole genome shotgun sequence.
ACCESSION   DASUTG010000007 DASUTG010000000
VERSION     DASUTG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297480"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1679)
                     /gene="groL"
                     /locus_tag="VF089_00145"
     CDS             complement(24..1679)
                     /gene="groL"
                     /locus_tag="VF089_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX6172390.1"
                     /translation="MASKIVKFNRDARDAMLRGVNILADAVTVTLGPKGRNVVLDKSF
                     GAPNVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARQIFAE
                     GVKLVAAGHDPMTLKRGIDKAVEAVTAELKNLSKPTKDPKEIAQVGTIAANNDSTIGD
                     VIAEAMNKVGKEGVITVEEAKGLETTLDVVEGMQFDRGYLSPYFVTDPERMECILEDA
                     YLLIHEKKISNMKELLPVLEQIAKSGKPFIIIAEDVEGEALATLVVNKIRGTLHCVAV
                     KAPGFGDRRKAMLEDIAILTGGKLIAEELGVKLENIALQDLGRAKRVVVDKDNTTIVD
                     GAGKKADLEGRIKQIRAQIEETTSDYDREKLQERLAKLIGGVAVINVGAATEVEMKEK
                     KARVEDALHATRAAIEEGIVPGGGVAILRAAAGLDKVSVDEDERWGVNIIKRVSEEPI
                     RRIAMNAGVEGSIVLQKVKEGKGAFGFNAATEQYEDLMKAGIIDATKVVRTALQNAAS
                     VASMLLTTEAMVAERPEEKSAMPPMPPGGMGGMGGMGGMGGMM"
     gene            complement(1707..1997)
                     /gene="groES"
                     /locus_tag="VF089_00150"
     CDS             complement(1707..1997)
                     /gene="groES"
                     /locus_tag="VF089_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6172391.1"
                     /translation="MKIRPLQDRIIVKRIEEEEKSKGGIIIPDTAKEKPQEGKVVAVG
                     KGKVSEDGKITPLDVKVNDRVLFGKYSGSEINIDGEEHLIMREDDVLGVLEK"
     gene            complement(2174..2452)
                     /locus_tag="VF089_00155"
     CDS             complement(2174..2452)
                     /locus_tag="VF089_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX6172392.1"
                     /translation="MSRLSESRISHMAHLVVNGLKRDDMAEFPNERLVLSETKRVLHE
                     FFEREDQFDELVRQKIQSLSRHIPPGSREWDVLYRKYLEEEMRRQKNK"
     gene            complement(2449..2724)
                     /locus_tag="VF089_00160"
     CDS             complement(2449..2724)
                     /locus_tag="VF089_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX6172393.1"
                     /translation="MKIKPEQLDKLSKLLLENYQAKELITPKRDDATIVTKIKGVISQ
                     NFAEEEAIEDEARKMLAAHAQATREMDHYKMFLIAKQKLAAKKGFIL"
     gene            complement(3046..3237)
                     /locus_tag="VF089_00165"
     CDS             complement(3046..3237)
                     /locus_tag="VF089_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172394.1"
                     /translation="MKKIDQVIGPVPFVSGKITLAVKYSGGQFDFEPLISDRLNSCKQ
                     SISLNTAGCCHNADAVPWL"
     gene            3397..3933
                     /locus_tag="VF089_00170"
     CDS             3397..3933
                     /locus_tag="VF089_00170"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX6172395.1"
                     /translation="MVTFHAPMVAMELARGLNGRTQEGFWGLLTGAKSKWEIELGEVI
                     RTGNAEGEMMGGCLSVLVTTLGTPYEIQTDGKLLFLEDVGERPYRIERMLTHLKMAGK
                     LDHLAGLIFGDFTNCDAEGPRTVLTIIDEMFAQAPYPVLTGMPAGHGREHITLPFGVK
                     MILDGNRRSLSLVESPVA"
     gene            3985..5109
                     /locus_tag="VF089_00175"
     CDS             3985..5109
                     /locus_tag="VF089_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6172396.1"
                     /translation="MTIDFHAVENATEQAVKQGVFPGAVLLVGRGEDVLYEQAFGWRS
                     LLPDRTAMRLSTLFDLSSLTKPLATTLGIMLLVNEKKIHLDDQVTQYLPAFANPATKI
                     RHLLNHTSGLPAWKPYYERVNRCAIGGRTGFDRSRSAKSYILEQVHRETSSGEAGTQA
                     LYSDLGFMVLGEIIEIISGSNLDLFFHERISQPLKLSAATFVDLTQPPIGRSQPKEDE
                     IAPTELCPWRKKVLCGEVHDDNAFAMGGVAGHAGLFSSARDIHAIVTQLDRCLRGCSS
                     FLPQWLVHEFFTKDDSVPQSTYALGWDTPAPENSSSGLHFSRRSVGHLGFTGTSLWWD
                     LEKNCHVILLTNRVHPNRNNEKIKQFRPKIHDLIMQAINP"
     gene            5106..6527
                     /gene="mpl"
                     /locus_tag="VF089_00180"
     CDS             5106..6527
                     /gene="mpl"
                     /locus_tag="VF089_00180"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX6172397.1"
                     /translation="MNELPAKGSHIHLIAVCGVGMASLAGLLKARGYRVTGSDQNVYP
                     PMSTYLAQAGIDVQIGFDPQHLASRPDLVIIGNAVSRDNPEAEAVLDGKIPYLSFPQA
                     LGRFLIGTRQSIVVAGTHGKTTTTALTAWVLKCAGLDPGFFIGGVPLNFGSGWAPGES
                     DHVVLEGDEYDSAFFDKGPKFLHYSPQSIILTSVEFDHADIYRDLDHLKEAFRSLTRL
                     IPTSGLLVACYDYLAVRDVVKAGSCRVISYGDNETCDWTVRNVRSEGRRSYFDPCFRN
                     ACEGAVELGAIGRHNVNNALGVYVLARELGIERSNLIEGFASFSGVKRRQEFKGEKRG
                     ILVIDDFAHHPTAVAETIKAVKDAFTGRRLWAIFEPRSNTSRRNIFEREFALALSLAD
                     RVVVSGLHQPEKIPEAERLSVQRVVESINQSNGHEQAVVINGADNIAAHVTARASPGD
                     IILVMSNGAFDGVQDKILLGLAS"
BASE COUNT         1477 a         1792 c         1757 g         1752 t
ORIGIN      
        1 cctgggtcgg tacagctttc tacttacatc atgccgccca ttccgcccat accccccatc
       61 ccacccatac cgcctggcgg cattggaggc atagctgact tttcttccgg tctctccgcg
      121 accattgctt cggtcgtcag aagcatactg gccactgagg ccgcgttctg cagcgcggta
      181 cgaaccacct tggtggcgtc gatgataccg gccttcatca aatcctcgta ctgttcagtg
      241 gccgcattaa agccgaaggc gcctttacct tcttttactt tctgcaacac gatcgatcct
      301 tcgacgcctg cgttcattgc aatgcgccga atcggttcct ctgaaactcg tttgatgatg
      361 ttgacgcccc agcgctcgtc ttcatcgacg cttaccttat ccaggccggc ggcggctcga
      421 aggatggcca ccccaccccc cggtacgatc ccttcttcga tcgcagcacg agtcgcgtgc
      481 aaggcatctt ccacccgagc ctttttctct ttcatttcaa cttcggtggc cgccccgaca
      541 ttgatgacag ccacgccccc gatcagcttg gcgagacgct cttggagctt ttcgcgatcg
      601 taatccgatg tggtctcctc gatttgagca cggatctgtt tgatacggcc ttctaggtcg
      661 gccttcttgc cggcgccatc gacgatagtc gtgttgtctt tgtcgacgac taccctcttg
      721 gcgcggccca agtcttgaag cgcgatattt tctagcttaa cccccagttc ctcggcaatc
      781 agcttgccac cggtgaggat ggcgatgtct tccagcattg ccttgcggcg atcgccaaaa
      841 cccggcgctt ttactgcgac acagtggagc gtgccgcgga tcttgttgac taccagagtc
      901 gcaagagctt cgccctccac gtcttcagca atgatgataa atggcttacc ggatttcgca
      961 atctgctcga gaaccggcag cagctctttc atattgctga ttttcttctc gtgaatcagc
     1021 aggtacgcgt cctcgagaat gcattccatt ctttccggat cggtcacaaa atacggcgag
     1081 agatagccgc gatcgaactg cataccttca acgacatcca aggtggtctc aagtcccttg
     1141 gcctcttcta ctgtgatgac accttctttg ccgaccttgt tcatagcttc ggcgataaca
     1201 tcgccgatgg tcgaatcgtt gtttgcagcg atggttccga cctgggcgat ttcctttgga
     1261 tctttagtgg gtttggaaag attcttcaat tctgcggtga ccgcttcgac agctttgtcg
     1321 atgccgcgct tcagagtcat gggatcatgg ccagcggcaa ccagctttac gccttcggca
     1381 aaaatctgcc gggccagaac agtggcggtg gtcgtcccgt caccggcgac atcggaggtc
     1441 ttactggcaa cctctttgac catctgcgca cccatgtttt caaatttgtc ttcgagttct
     1501 atctctttgg ctacggtgac tccatctttg gtgacgttcg gtgcgccaaa ggatttgtcc
     1561 aatacgacgt ttctaccttt gggccctaac gtgaccgtga ctgcgtcggc tagaatgttg
     1621 acgcccctca acatcgcgtc tctggcgtcg cgattaaact tgactatctt agatgccatc
     1681 cttcttcctc ctgattagat tggtaattac ttttcaagga cgccgagaac atcatcttcg
     1741 cgcatgatta aatgttcttc gccgtcgata tttatttcgc ttccggaata ttttccaaag
     1801 agcacgcgat cattgacttt cacgtccaga ggggtgattt tgccatcctc gcttaccttt
     1861 cccttcccca cggccaccac ttttccttcc tgcggctttt ctttggcggt atccgggatg
     1921 atgatgccac ccttgctttt ctcttcctcc tcgatacgtt tcacaatgat gcgatcctgc
     1981 aaaggcctaa ttttcacctt ttgcctcctt tcgtgtgttt agattaagtg ctagttggat
     2041 tctagtcagg cggtccggct gcgcgtcagc gataaattgg accgtcaaag cgggatcaat
     2101 ctaagcatca aacccgccgt gtcaaggtga tccgggagaa aagttgatta ccttcgaagg
     2161 caaagatggg tcgtcacttg tttttctgac gtctcatctc ctcttcgaga tacttacgat
     2221 acagcacgtc ccattctcgg cttcctggtg ggatatgccg ggataaagac tggatttttt
     2281 gccggactaa ctcgtcgaat tgatcttcgc gttcgaaaaa ttcatgtaag acgcgtttgg
     2341 tttcagaaag caccaggcgc tcgttcggga actcggccat gtcgtcgcgt ttgagtccgt
     2401 taacgaccag gtgggccata tgtgagattc ggctctcgga gagtcggctc atagaataaa
     2461 gcccttcttg gctgccaact tctgtttggc aattaagaac atcttatagt ggtccatctc
     2521 gcgagttgcc tgagcatgag ctgcgagcat cttgcgcgcc tcgtcctcta tagcttcttc
     2581 ttcggcaaag ttctggctga tcaccccctt gatcttggtc acgatcgtcg cgtcatcgcg
     2641 tttaggcgtt attaactcct tagcctgata gttttcaagg agcagcttgg aaagtttatc
     2701 gagctgttcc ggtttgattt tcatgctgtg gaaaacgttg ttgataagtt gctaacagcc
     2761 agtgtgaagc tcgggcagaa gcagtgaatc aactgatttt ctacccgcgt tagcggcgga
     2821 tgtcaaccgc acgatcgtca catcgcaaga tcgaggataa gcaccatgga taaaaaatat
     2881 cttgtgaatt taagggtttt ttgtcactat gagaggtgtt cgtgagatct tcccgccggc
     2941 aattctagca aaatgcggaa ttatccacag gtaaaacatc agactgattt gaacggtgtt
     3001 cgagaggttg tgaacaacta ggtgacgctt agaaaaccag cccggttaca gccagggcac
     3061 cgcatcggcg ttatggcaac agccggctgt gttgagcgag atgctctgct tacaggagtt
     3121 caggcgatcc gagatcaggg gttcgaagtc gaattgtccg cccgaatatt tgacagcaag
     3181 ggttatcttg ccggagacga acggaaccgg gccgatgacc tgatcaattt ttttcaaaga
     3241 agtgatatta gcgcgatctt ctgcgctcgt ggaggctttg gttcagcaca acttttgccc
     3301 catttggtac actcgaattt tcagcctgct ccgaaaattt ttgccgggta tagcgatgtt
     3361 acgtttctgc tgaactggat aaacagccgg tttggcatgg taacctttca tgcgccgatg
     3421 gttgcgatgg agctagcgcg tggccttaac ggccgcactc aagaaggttt ctggggattg
     3481 ctcaccggcg ccaaatccaa atgggaaatt gagttaggcg aggtcatccg cacgggaaac
     3541 gccgagggtg agatgatggg aggctgttta tccgttctag tgaccacgct agggacgccg
     3601 tacgagatcc aaacggatgg caaactgctg tttttggaag acgttggtga acggccttat
     3661 cgaatagagc gaatgctgac ccatctgaaa atggccggaa agctcgatca cctcgccggc
     3721 ttaatcttcg gcgacttcac caactgtgac gccgaagggc ccagaaccgt gctgactatt
     3781 atcgacgaaa tgttcgcgca ggctccgtat ccagtattaa cgggaatgcc ggctgggcat
     3841 ggaagggagc acataaccct gcctttcggt gtcaaaatga tactcgacgg aaaccgccga
     3901 tctctttcgc tggtcgagtc tccagtcgct tagaactaaa aaccaaagtt ctacgcgtca
     3961 gcccgtctaa ccaaagttca ttctatgact atcgattttc atgccgttga gaacgcgacc
     4021 gagcaggcag tgaagcaggg ggtatttccc ggcgcggtac tgctcgttgg tcgtggagaa
     4081 gatgtgctct atgaacaagc tttcggatgg cgcagtttac ttcccgatcg gactgcgatg
     4141 aggttatcga cgcttttcga cctttcatcc ttaaccaagc ctctagcgac gacgctcgga
     4201 atcatgctcc tggtcaatga gaagaaaatt catcttgacg atcaggtaac tcaatacctg
     4261 ccggctttcg caaatccggc cacaaaaatc cggcacttgc taaatcatac atccggcctg
     4321 cctgcctgga agccttatta tgaacgcgtg aaccggtgcg caattggtgg aaggaccggc
     4381 ttcgaccgaa gccggtcggc caaaagctac atcttggaac aagttcatcg ggaaacgtca
     4441 tcaggtgagg cgggcacgca agctctttac agcgatctcg gattcatggt ccttggcgaa
     4501 ataattgaaa tcatcagcgg ctcaaatttg gatctctttt ttcatgagcg gatttcccag
     4561 ccattgaaat taagcgcggc aacttttgtc gacttgacgc aacctccaat agggcgaagt
     4621 cagccgaaag aggatgaaat cgctcctaca gaactttgcc cctggcgcaa aaaagttctc
     4681 tgcggcgagg tgcacgatga taatgctttt gcaatgggtg gggttgccgg gcatgccggt
     4741 ttgttctcct cagcgcggga catacatgcc atagtgactc agttggatcg ctgcctgcgc
     4801 ggttgcagct cattcctacc gcagtggcta gttcacgagt tctttacgaa ggacgattcc
     4861 gtgcctcaat ccacgtacgc attgggttgg gatactcctg cgccggaaaa ttcctccagc
     4921 ggactccact tctcgcgcag gtctgtcggg caccttggct tcactggcac ctctctctgg
     4981 tgggacttgg agaagaattg ccatgtgatt ttactgacca atcgcgttca cccgaaccgt
     5041 aacaatgaaa aaattaagca gtttcgaccg aagattcacg atctgattat gcaggcgatc
     5101 aatccatgaa cgaactacca gctaaaggaa gtcacataca cttaatcgcc gtatgcggcg
     5161 tcggtatggc gtcattggcc ggccttctta aggctcgggg ttatcgtgtc accggctcgg
     5221 atcagaatgt ctatccgccg atgagcacct atttggcgca agccggcatc gatgtacaga
     5281 ttggattcga tccgcaacat cttgccagcc ggcctgactt ggtgattatc ggcaacgctg
     5341 tttcacggga caaccctgaa gcagaagctg ttctggacgg caagatccct tatctttcat
     5401 tcccacaagc tctgggaaga ttcttgatcg gcacccgcca gtcgatcgtt gtcgctggaa
     5461 cacacggcaa aacgaccacc acggctttga cggcttgggt gctgaaatgc gctggtcttg
     5521 acccgggatt ttttatcggc ggtgtgccgc tgaattttgg cagtggttgg gctccagggg
     5581 agagcgacca tgtggtgttg gagggcgacg aatacgacag cgccttcttt gataaaggcc
     5641 ccaagtttct tcactacagc ccgcaaagca tcattcttac cagcgtagag tttgatcacg
     5701 cagacattta tcgcgatttg gatcacttga aggaagcgtt tcgatctctg actcggctca
     5761 tccccaccag cggattgcta gttgcctgct atgattatct tgccgtccga gatgtcgtga
     5821 aagccgggtc ctgccgggtc atatcttatg gcgataatga aacctgtgat tggacggtgc
     5881 gcaatgtccg aagcgaaggg cgccggagtt actttgatcc gtgttttaga aatgcttgtg
     5941 agggggctgt ggagctcggt gcgatcggcc ggcataacgt gaacaatgcc ctgggtgtct
     6001 atgtgttggc ccgagaatta ggaatcgagc ggagtaatct gatcgaaggc ttcgcatcgt
     6061 tttcaggcgt caaacggcgc caggagttta aaggggagaa acgcggcatt ttggtcattg
     6121 acgactttgc gcatcaccct accgcggtag cggaaacaat caaagcggtt aaagacgcct
     6181 tcaccggacg aagactctgg gcgatttttg aaccgcggag taataccagc cggcgcaaca
     6241 tcttcgagcg cgagtttgct ctggcattgt ctctggccga tcgggtcgtg gtttccggcc
     6301 ttcatcagcc cgagaagatt cccgaggcag aacgattatc cgtccaacgt gtcgtcgagt
     6361 cgatcaatca atccaatggg cacgagcagg cggtagtcat caacggtgcg gacaacattg
     6421 ccgctcatgt gactgcccgc gccagccccg gcgatatcat actggtgatg tcaaacggcg
     6481 ctttcgacgg ggtgcaggat aagattttat tggggctagc gagttaggtg caaggcggcg
     6541 catttctcac tgcaaaagta atttcctttt cgtagtaacg cctcgctctt tggaacatag
     6601 ctctgacatt gcggatccaa gaccatctct tcgcccttag gcctggccag gtttggattg
     6661 cgcccagagc ctttgcgggc gatgaaatag gctcggagcg cggtaaatac aatccatgcg
     6721 acaaggacaa atagaagcag tcgtaccagc actatggtta tctaacaggg cccgactc
//
LOCUS       DASUTG010000008         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1928146, whole genome shotgun sequence.
ACCESSION   DASUTG010000008 DASUTG010000000
VERSION     DASUTG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928146"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1957
                     /locus_tag="VF089_00185"
     CDS             152..1957
                     /locus_tag="VF089_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6172398.1"
                     /translation="MAERNTTAAGPEVWSAGVGRKSNAQYGSDLMVEVLRELGIKYIA
                     LNPGASYRGLHDSLVNFEAGKGPEIIMCTHEEIAVALANGYARVTGDIMATGLHNVVG
                     LQHASMAIFNAWCDRTPILNLGGGGPQNATHRRSTDWVHTALVQGNAVRDYVKYDDQP
                     NSVDAIPESFLRAYRIAMTEPRGPVYICLDTDVQEEKISKPMVVPHAQLFRPPAGPAA
                     NPESLRQASRLLAEAQWPVIVAGELGRNPKALPPLLDIAEELGAAVIDSDGRYAFPST
                     HPLNLTNAREEALRDADVVLALDVPSLGVPLGPSVRERGNFAPVISPSCKVIHMTLLD
                     LERQSWVSDAMWLLPVHVPIAADTAVALPQLLEQLRERMKNNAESARQVRERRAKIEA
                     IYNQAREKSQEWIEKTWDEKPISQARFFGEINKRMQGKAWALVSAHGRRWREVIEVTE
                     PAHGIGGGRGGGVGYGLPSSIGAALGHKESGRLCVSIIGDGDFLMTSNALWTAAKYQI
                     PLLVLVMNNRSYYNDEEHQERMAKWRQRPVENKGIGIRIEDPAPDLAAISRALSVEAF
                     GPITEPDQLGSTLDKAIAIVEGGKPAVVDIVTQPR"
     gene            2129..2320
                     /locus_tag="VF089_00190"
     CDS             2129..2320
                     /locus_tag="VF089_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172399.1"
                     /translation="MDKIIEQSLMAQLAREGLEPRPGDLDEFAKIIELYLDNLKTLHS
                     VNLGAEELAPVFRPDWTGK"
     gene            2445..3737
                     /locus_tag="VF089_00195"
     CDS             2445..3737
                     /locus_tag="VF089_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6172400.1"
                     /translation="MAVAEKPLYYLSIHEAQQLIQKRQLSPVELTQAVLERIQAIDDK
                     LHAYINLMPDSAMQEARHAETEIHKGNWRGPMHGIPIAVKDQLDVEGAQARIRQFTTG
                     VGDATAVKKLREAGAIIMGKLHMSSLPDGSLPVPRNPWNTEHITGGSSTGSGAAVSGG
                     LCLGSLGEDTAGSIRNPSAFCGIAGLKATYGRVSRYGLAPLSWSLDHCGPMARVVEDT
                     AYMLQAIAGHDAKDPTSSNVPAADYASALREDVRGMTVGVPRDYIDECAPRTDPIVIR
                     LVDRAIEDLKKLGARIEEVKVPTLNLATIANAVIYYNEFWAAHKSDAASVLKNGAAQR
                     RARIYLGLLTGSADYIQAQRVRSRVRREFAEVFEKVDCLALPCQAGPAPLVKDVGPID
                     TLFKHVVPEYHGPFNLTGLPTLSIPCGFSDNHCRLLCN"
     gene            complement(3734..>3944)
                     /locus_tag="VF089_00200"
     CDS             complement(3734..>3944)
                     /locus_tag="VF089_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172401.1"
                     /translation="PKSAALQFHSRGLIRCISMISFLLLRFELLERNNGLNFSERRTL
                     LVPLRTLLVGVSRSQDRHFIERLAD"
BASE COUNT          901 a         1066 c         1189 g          788 t
ORIGIN      
        1 agcttttgca ttttcgatat ctccgctgcc aagaaacata ttggttacga gcccgcgtat
       61 gatgttaaaa aaggcgttcg cgattatgtc gaaaccatca aggtgctgag gtcatgagca
      121 acgagtttaa gcattagatc acggaggaat aatggcagaa cgaaacacaa cagcggccgg
      181 cccggaggta tggtcggcgg gcgtgggcag aaaaagcaat gcgcaatatg gctcggatct
      241 aatggtcgaa gtgctgcgcg agctgggaat caaatacatc gccttgaatc ccggcgccag
      301 ctatcgcggt ctgcacgatt ccctggtgaa cttcgaggcg ggcaagggtc ccgagatcat
      361 catgtgtacc cacgaagaga tcgccgtagc attggccaat ggctatgccc gggtcaccgg
      421 cgatatcatg gccaccggtc tgcacaatgt tgtcggcctg caacatgcca gcatggcgat
      481 tttcaatgcc tggtgcgacc gcacgccgat cttgaatctc ggcggcggcg gcccgcagaa
      541 cgcaacgcat cgccgctcca cggattgggt gcataccgct ctggtgcagg gcaatgccgt
      601 acgcgattac gtcaagtatg acgatcagcc gaattcagtc gacgcgattc ctgaatcgtt
      661 tctgcgcgcc tatcggattg ccatgaccga acccagaggt ccggtttata tctgcctcga
      721 taccgatgtt caggaagaga aaatcagcaa accgatggtg gtgccgcatg cccagttatt
      781 tcgcccgccg gcaggcccgg cagccaatcc cgaatcgctg cggcaggcat cgcgactgct
      841 cgccgaagcg cagtggccgg taatcgttgc cggcgaactc ggacgcaatc ccaaggcgct
      901 gccgcctctg ctcgacatcg ccgaggaact cggtgcggcg gtgatcgatt cggatggccg
      961 ctatgcattt cccagcacgc acccgcttaa tcttaccaac gcacgcgagg aagccttgcg
     1021 cgatgccgac gtcgttctcg cattggatgt gccgagtttg ggagtgccgc tcggcccgtc
     1081 ggtgcgcgag cgcggcaatt tcgcgccggt gatttcgccc agctgcaaag tgattcatat
     1141 gaccctgctc gatctcgaac ggcagagttg ggtgagcgat gccatgtggc tcttgccggt
     1201 gcacgtgccg atcgccgccg ataccgcggt ggcgttaccg caactgctcg agcagttacg
     1261 cgagcggatg aagaataatg cggaatcggc tcggcaggtt cgtgagcgcc gcgcaaagat
     1321 cgaagccatc tataatcaag cgcgcgagaa gagccaggaa tggatagaga aaacctggga
     1381 tgaaaaaccg atttcgcaag cgcgcttctt tggcgagatc aacaagcgaa tgcaaggcaa
     1441 ggcgtgggcg ctggtatcgg cgcacgggcg gcgttggcgt gaagtgatcg aagtcacgga
     1501 accggcgcac ggcatcggcg gcggcagggg aggcggggtc ggctacggct tgccgtcatc
     1561 gattggcgcc gcgttggggc acaaagaaag cggcaggtta tgcgtcagca tcatcggcga
     1621 cggcgatttt ttaatgactt cgaacgcctt atggacggcg gccaagtatc agatcccttt
     1681 gctggtgctg gtcatgaaca atcgctctta ctacaacgac gaagaacatc aggagcggat
     1741 ggccaaatgg cggcaacgac cagtcgaaaa taaaggtatc ggcattcgca tcgaggatcc
     1801 agctcccgat ctcgcggcaa tttcccgggc gttgagcgtc gaagctttcg ggccgattac
     1861 cgaaccggat caactcggct ccactctaga caaggcaatt gccatcgtcg agggaggaaa
     1921 gccggcagtc gtagatattg tcacacagcc gcgctgagga ccgcgcgccc cgggctgttg
     1981 tcgaatgtgt gttgatcgat gtcgttttga taaagtcggt ggcgagctta ccgcgtcacc
     2041 gttttgttaa cctagtttgt ttgaactaac ggcgatagag tcggcgccgc aaagcaaatc
     2101 gcctcaacca ctgggaagga gaaggcatat ggacaaaatc attgagcaaa gcctgatggc
     2161 gcagcttgcg cgcgaaggtt tggagccgag accgggcgac ctcgacgagt ttgccaagat
     2221 catcgagctt tacctcgaca acttgaagac cttgcattcg gttaatctag gtgcggagga
     2281 gctggcgcct gtttttcgac cggactggac cgggaagtga ttgaatgaca ccggatgcct
     2341 ggcttgtttg gaacgacggc gccagagttg tcaccggaac aaagtgaatg atccgttttt
     2401 gtcacgcaat tagttgacac cgcaaagctg caaaggagac tgccatggct gtagccgaga
     2461 agccgctcta ttacctgtcg attcacgaag ctcaacaatt gattcaaaag cgccagctct
     2521 cgccggtcga attgactcag gcggtgctcg agcgcattca ggcgatcgac gacaagctgc
     2581 atgcttatat caatttaatg cccgacagcg ccatgcagga agctcgtcac gccgagacgg
     2641 aaatccacaa gggcaactgg cgcggtccga tgcacggcat ccctattgcc gtcaaagacc
     2701 aattggacgt ggaaggcgcg caggcgcgga ttcgccaatt tactacggga gtcggtgatg
     2761 ccaccgctgt gaaaaagctc cgcgaagcgg gcgcgattat tatgggcaag cttcacatga
     2821 gcagtttgcc tgacggatcg cttccggtgc cgcgcaatcc ctggaacacc gaacacatca
     2881 ccggcgggtc gagcacgggt tctggcgctg ccgtttccgg cggactctgt ctgggctctc
     2941 tgggcgaaga taccgcaggg tcgattcgca atccgtcggc tttctgcggc atcgccgggc
     3001 ttaaggcgac ttacggccgg gtgagccgtt acgggctagc gccgctcagc tggtcattgg
     3061 accactgcgg tccgatggcg cgtgtggtcg aagataccgc ctatatgttg caggcgatcg
     3121 ccggccatga tgccaaagac ccgacatcga gcaacgttcc ggcggccgat tatgccagcg
     3181 cattgcgcga agacgtcaga ggaatgacag tcggcgtgcc gcgagactat atcgacgaat
     3241 gcgcgccgcg caccgatccc atcgtcatca gactggtcga ccgagccatt gaagatttga
     3301 aaaaattggg cgcgcgcatc gaggaagtta aagtgccgac attgaatctc gcaacgatag
     3361 ccaatgcggt tatctattac aacgaattct gggcggcgca taagagcgat gccgcctcgg
     3421 tgctgaagaa cggcgcggcg caacgacggg cgcgcattta cctggggcta ctcaccggct
     3481 cggcggatta catccaggcg cagcgcgttc gaagtcgtgt ccgaagagaa tttgccgaag
     3541 tcttcgaaaa agtggattgt ctcgcgttgc catgccaggc ggggccggcg ccgctggtga
     3601 aagacgtggg cccgatcgac acattgttca aacatgtggt tcccgaatac cacgggcctt
     3661 ttaatctaac gggattgccg acgttgtcga ttccttgcgg attctcagac aatcattgcc
     3721 gattgctctg caactagtcg gcaagccgtt cgatgaagtg acgatcttgc gagcgggata
     3781 cacctaccag caaagtgcga agtggtacca gcagcgtccg ccgctctgaa aaattcaagc
     3841 cgttattacg ttcaagcagt tcaaacctta aaaggagaaa agagatcatg gatatacaac
     3901 ggatcaatcc gcgggaatgg aactgtagcg cggcactctt tgga
//
LOCUS       DASUTG010000009         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_445806, whole genome shotgun sequence.
ACCESSION   DASUTG010000009 DASUTG010000000
VERSION     DASUTG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445806"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VF089_00205"
     CDS             <1..754
                     /locus_tag="VF089_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6172402.1"
                     /translation="KTLATRDFCNVLIDEVKNGHARLHHPFYADLYDGKLPLDAVKIW
                     AKEAWGIFAYNVAINTAKLVRCQLSGIHDPEIHKTFVDIIHSEVGYKYFEGSPRPVPG
                     HRALFLRFGESIGIPAQELERCEREEDFLPTTVLARIGWLDIALRSNHILEQVAATNC
                     CNEFSNQLTGGRFFRAFNRHYGLSSNDIEFFAEHGEADTEHSNIGYELVEQFATTNEL
                     QVRVLKALRKGLSIWWALTDGVARECARKHLH"
     gene            800..1357
                     /gene="nbaC"
                     /locus_tag="VF089_00210"
     CDS             800..1357
                     /gene="nbaC"
                     /locus_tag="VF089_00210"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX6172403.1"
                     /translation="METLSTFNLKRWVNENQHLFNPPLKTNQLIVHHKDFMVMVLRGP
                     NTRLDFHIEPGDEFFYQIEGDMELHLKPPGERREVVTIKQGEIFVCPGDLPHSPRRFE
                     NTWGLVIERKRRDEEKEEFAWFCEKCDELILSRVVNQGNIPSQVSAVYQEFNDNEQIR
                     TCQACGYVFPPTPMAERLAFLEPKK"
     gene            1589..>2269
                     /locus_tag="VF089_00215"
     CDS             1589..>2269
                     /locus_tag="VF089_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6172404.1"
                     /translation="MAIGHLSLRQAVIEGANMLVRVGAISMSHHGNFSVRVPNTDTIL
                     LTATSSFDSLNPENLALLDLDGRLLEGEINPTNAEIVHMHAVVYKQRPETGAVVHTHS
                     TYATSFALASRSLSCSYEALVRADMTDGVPLARYGPRGSAASVDNIAIALSKATNTKA
                     VLLENHGVLAFGADPAAAARANLIVEEAAQMAIFAEVLGGAKEIPAHLLKATVARRDD
                     FARSGPKRT"
BASE COUNT          530 a          596 c          628 g          515 t
ORIGIN      
        1 caaaacttta gcgactcgag atttctgtaa tgtacttatc gatgaggtca agaatgggca
       61 tgcccgtctg caccacccgt tctatgcgga tctttatgac ggaaaattgc cgttggacgc
      121 ggtcaagatt tgggcgaaag aagcttgggg aatctttgct tacaacgtgg cgatcaatac
      181 ggcgaaacta gttcgttgcc agttatcggg gattcacgat ccggaaattc acaagacatt
      241 tgtcgacatc attcactccg aggtcggcta taagtacttt gaaggcagcc cgcggcccgt
      301 gcccggccat cgggcattat ttctccgttt cggcgagagc atcggaattc cagcgcagga
      361 actggagcgt tgcgagcgtg aagaagactt tttaccgacg acggtgttag cgcgcatcgg
      421 ctggctcgat attgccctgc gcagcaatca tattctcgag caagtggcag cgaccaactg
      481 ctgcaatgag ttttccaatc aactgaccgg cggccgattc tttagagcgt tcaaccgcca
      541 ctatggactg agttcgaacg atatcgaatt ctttgccgaa catggcgaag cggacaccga
      601 gcacagcaat attggttacg agctggtcga gcagtttgcc accaccaatg agctgcaggt
      661 cagagtgctc aaggcgctgc gtaagggctt atcgatttgg tgggcactaa ccgacggagt
      721 cgcgcgggag tgcgccagaa aacatctgca ttgatcctag tgattgctcg cggggatttg
      781 agtaactgag tatgaggcga tggaaacgct ttcgacattt aatttaaagc gctgggtcaa
      841 tgagaatcag cacctcttta accctccgct caaaaccaat caactcatcg tccatcacaa
      901 agatttcatg gtgatggttc tccgcggccc gaacactcgg ctcgattttc atatcgaacc
      961 cggtgacgag ttcttctatc aaatcgaagg cgacatggaa ctgcatctga agccgccggg
     1021 cgagaggcga gaggtggtta cgatcaaaca gggagaaatt ttcgtgtgtc ccggtgatct
     1081 gccgcattcg ccgcgtcgtt ttgaaaacac ctggggactg gttattgagc gcaaacgccg
     1141 cgatgaagaa aaagaagagt ttgcctggtt ctgcgagaaa tgcgacgaac tgatactcag
     1201 tcgagtcgtg aatcagggca acattccgag tcaggtctcc gctgtctatc aggagttcaa
     1261 tgacaacgaa cagatccgga cctgccaggc ctgcggatat gtttttccac cgacaccgat
     1321 ggcggaacgg ctggcttttc tggaacccaa gaaatgagcg atgttgttgc gggttgtgaa
     1381 cgtctcgaca tcatatcccg ctgtgacttt gatacggcag gagaagaatc cgccgctgcc
     1441 cagtcatata caggaacaaa tggcaagatc catcgacagt cagtttcgcg cctgagcata
     1501 cagtgggcac tttatcgatc gtccttcggt atctatcggc aaactgtttt cgggctgatc
     1561 cacgcccggt gtaagcagag gaggatttat ggctatcggt catctatcat tgcgccaagc
     1621 ggtcatcgaa ggtgcgaata tgcttgtccg cgtcggcgcg atctcgatga gccaccacgg
     1681 caacttcagc gtgcgcgtgc cgaacaccga tacgattttg ctcaccgcca ccagctcttt
     1741 cgatagtctc aatcccgaaa atctggcgct actcgatctc gatggacgac tcctcgaagg
     1801 tgagatcaat ccgaccaatg ctgagatcgt ccatatgcac gcagtggttt ataagcagcg
     1861 tcctgaaacg ggagccgtgg tgcacactca ttccacttac gccaccagct tcgcgctggc
     1921 cagccgatcc ctatcctgct cttatgaggc cttggtgcgc gcagatatga ctgacggtgt
     1981 gcctctggct cgttacggcc cacgaggctc ggcggcatcg gtggacaaca tcgcgatcgc
     2041 tttgagcaag gcgactaaca ccaaggcagt gctgttggaa aatcatgggg tgcttgcctt
     2101 tggcgcagac ccagcggcag ccgcgcgggc caacctcatc gttgaggagg ctgcccagat
     2161 ggcgattttc gcggaggtgc tgggtggcgc gaaagaaatc ccggctcacc ttttaaaggc
     2221 gacagtcgca cgacgggacg attttgctcg ctccggcccc aagcggacc
//
LOCUS       DASUTG010000010         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1172, whole genome shotgun sequence.
ACCESSION   DASUTG010000010 DASUTG010000000
VERSION     DASUTG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1706
                     /locus_tag="VF089_00220"
     CDS             435..1706
                     /locus_tag="VF089_00220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6172405.1"
                     /translation="MFGELRDRLAGLFYGWRMVGVSCAIRLLGGGLHAYGFTVFFLPI
                     TQELGLSRAATSLVFSLARAQGAIEGPVAGYCIDRYGPRPVIFVATLLAGIGYMLLAG
                     VQSYAALLIVYMGIISLSYQAGFMDATMVVANSWFIRKRALAMSLTSGSIALGGTLFT
                     PFLAYAVHTWGWRSAAMGAGVAFLIGGLPLSLLLRRSPESMGLLPDGEPLPKATDQEG
                     STRPTQEANFSLQQTLRLRQFWLLSIATTFRVVCSSCVLVHFVPILVWRGLTEQHAAF
                     FLAFIALLGMPAHLLIGWLGDRLNKPRLMATCMIIASLSVYLLFSNTAEWQLWLALVL
                     FTVVEGLFPVTWATVGDFFGRSNFAKVRGSMSFLYMWGSVIGPVIAGAVYDRTQSYSP
                     LMWGLISLCWATALIYAVLVKPSSASSPANL"
     gene            1712..2152
                     /locus_tag="VF089_00225"
     CDS             1712..2152
                     /locus_tag="VF089_00225"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX6172406.1"
                     /translation="MSKHNRIKFVNKWLPVLLWATVIFLFSTEHFAAPQSSRILGPLL
                     NWLFPGITPEQLASVQFVVRKFGHWFEYSVLAVLLYRALYAEAGGRSSVRPAAMTMAF
                     ALVWAITDEFHQSLVPSRTASIVDVMIDGFGALCGSFWMYLRHR"
     gene            2305..>2704
                     /locus_tag="VF089_00230"
     CDS             2305..>2704
                     /locus_tag="VF089_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6172407.1"
                     /translation="MTFLNRLAGLLILLSLSACSSSGSGPTITATNFMPPELLKGASY
                     SPSAMEALSRGLAVITPPGAALQDVYYEFDSVDLKAEAEAILKKNAEWMKANPLARVE
                     VEGHCDDVGSAEYNLALGAKRAQTARDFLVQ"
BASE COUNT          508 a          770 c          748 g          678 t
ORIGIN      
        1 ctttttttac ttgtggggtc ctgcgctggg gcctgtggtc acgggcgcga tctacgatcg
       61 ctatcagagt tacgcgccga tgatgtcggc gtatatcgtc ctgtcggtga tcgcgggttg
      121 cctctatgcg atgatgtcgc ggcccaggcc gcgatagtca gggcgctgct gaaaaaagtt
      181 cgtagactcg attgcgcttg cccgaagcgc ttcaacgtac tgaagagtac cgcagctcat
      241 ccaaattggc tgcgtagcct gcgagacctt tttgaagcag ccgtaattga agtgtagaac
      301 cagcgttgat caggtaaatt cagccaatgc attgacaggc caatcgtagg tcagctactt
      361 ttatcgatgg cacaaaatcc cggcacagac cccactggaa actttttcat ttaacgcccg
      421 cattgtattg atccatgttt ggagaacttc gcgaccggct ggccggtctt ttctatggct
      481 ggcgcatggt cggcgtcagc tgcgctatcc gccttctcgg cggcgggctc catgcttatg
      541 gttttaccgt tttctttcta cccatcactc aagagttagg cttgtcacgg gccgcgactt
      601 cgctggtctt ctctctagcg cgcgcccagg gcgcgatcga aggaccggtg gccggctatt
      661 gcatcgaccg gtatggaccg cggccggtga ttttcgtcgc aactctgctc gcgggcattg
      721 gctacatgct cttggccggg gttcaaagtt atgccgcttt gctgatcgtc tacatgggga
      781 ttatctcgct ctcctatcaa gccggattca tggatgccac catggtggtc gccaacagct
      841 ggtttattcg caaacgagct ctagccatgt cgctcaccag cggttccatc gcgcttggcg
      901 gcacgctgtt cacgccgttt ctcgcttacg cggtgcacac ctggggttgg cgcagcgctg
      961 cgatgggcgc cggagtggcg ttcttgattg gtggcctgcc gctgtcgctg ctgctgcgcc
     1021 gctcgcctga gagcatgggc ctgctgccgg atggcgagcc gttgcctaaa gcgacggacc
     1081 aagaaggatc aacccgcccc acccaagaag ctaatttttc tctgcagcaa accttgcgcc
     1141 tgcgccagtt ctggctgctt tcaatcgcca ccacattccg cgtcgtttgc tccagctgtg
     1201 tcctggtcca tttcgtgccg atcctcgtct ggcgcggttt gaccgagcaa cacgccgcct
     1261 ttttcctcgc ctttatcgct ctgctcggca tgcccgcgca tctcctgatt ggctggcttg
     1321 gcgatcgcct gaacaagccc aggctaatgg ccacatgtat gatcattgca agtttgtcgg
     1381 tgtacttgct gttctcgaac acggcggaat ggcaactctg gttggcgctg gtcctcttca
     1441 ccgtcgtcga agggcttttt ccggttacct gggccacggt aggcgatttt tttgggcgga
     1501 gtaattttgc caaagttcgc ggcagcatga gttttcttta catgtggggt agcgtcatag
     1561 gtcccgtaat cgccggcgcc gtgtacgatc gcacgcaaag ctacagtcca ttgatgtggg
     1621 gactgatcag tttgtgttgg gcgacggcgc tcatctatgc tgtactggtc aaaccgagct
     1681 ccgcctcttc tccagctaac ttgtgaatca attgagcaag cacaaccgca tcaagttcgt
     1741 caacaaatgg ctaccggtgc tcctgtgggc gaccgtgatc tttttatttt ccaccgagca
     1801 ctttgcagcg ccgcaatctt ctcgcatatt gggaccgctg ctgaactggc tcttcccggg
     1861 cattaccccc gagcaattgg cctccgtaca atttgtcgtt cgcaagttcg gtcattggtt
     1921 tgaatactcc gtgctggccg tgttgctcta tcgcgctttg tacgctgaag ccggcggcag
     1981 aagctcggtt cgccctgccg caatgacgat ggcatttgcg ctggtctggg caataactga
     2041 cgagtttcac caatctcttg tgccgagtcg aaccgcaagc attgtcgacg tcatgatcga
     2101 cggcttcggg gcactttgtg gaagtttttg gatgtacctg cgccacagat gagcgagaga
     2161 gcccaacttg cgccgcagcg cgcgaaggaa aggcctgtac ggattgaaaa aacttgacaa
     2221 gcaaacggta actatccacg atactcagga cggtgatttc gcctgaaaaa ccctgatgag
     2281 acaacccatg atccttcccc ctcgatgaca tttttaaatc gtttggccgg cctgctcatt
     2341 cttcttagcc tctctgcgtg ctcgagctcg ggcagcggtc cgacaatcac cgcgacaaac
     2401 ttcatgcccc cggaactcct aaagggagcc tcatattcgc cgtcggctat ggaggcgttg
     2461 agccgcggcc tggccgttat cactccccca ggtgcggcgc tgcaagacgt ttattacgaa
     2521 tttgatagcg tcgatctgaa agccgaggcc gaggctattc tcaagaagaa tgccgaatgg
     2581 atgaaagcca atccgttggc ccgggttgaa gtcgaaggac actgtgacga cgtcggatca
     2641 gccgaatata acctcgcgct gggcgccaaa cgcgctcaaa cggccagaga ttttcttgtg
     2701 cagg
//
LOCUS       DASUTG010000011         4211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_890795, whole genome shotgun sequence.
ACCESSION   DASUTG010000011 DASUTG010000000
VERSION     DASUTG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890795"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            965..1114
                     /locus_tag="VF089_00235"
     CDS             965..1114
                     /locus_tag="VF089_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172408.1"
                     /translation="MSISIDTFSDDYWTSPAQRLRQVVKGGGLSLRSEGGVISTAAED
                     RPSAS"
     gene            2644..>4211
                     /locus_tag="VF089_00240"
     CDS             2644..>4211
                     /locus_tag="VF089_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX6172409.1"
                     /translation="MIKIKQLSKAYFARELFTDVSLQMNAGDRLGLVGRNGHGKSTLF
                     KLILGQEDPDSGEITIPRNYRIGHLEQHLRFTEPTILAEAALGLPEDESHSLYKAEAI
                     LFGLGFSAADLEKAPREFSGGFQIRINLAKLLVSEPNLLLLDEPTNYLDITSVRWIAR
                     FLASFNGELIVISHDRDFMDRVTTHTAVIHRQKIRKFEGGTATAYAQIFLEDEIHEKT
                     RANKDSKRAHAEAFINRFRAQASKAKMVQSRIKMLEKMPKLDQLTEIESLDFEFRHAP
                     FAAKTLLEARGISFGYTREHPLFQYLDLSVNRRDRIAVIGNNGKGKSTLLNVLSGGLQ
                     PLTGELKAHPDMKLGYFGQTNISRLNPQLTIEQEIEHSNPKLTRTQVRNICGTMMFGG
                     DLALKKISVLSGGEKSRTLLGKILAHPSNLLLLDEPNNHLDMESIDALIESLQDFPGA
                     LLIVTHNERILRALATRLVVFQRGRVDVFNIGYGEFLGKIGWEDETDGRGANKPTSRI
                     NYNEKREREKAERR"
BASE COUNT         1102 a         1102 c         1098 g          909 t
ORIGIN      
        1 gggagataaa acacacccgt tgaagcgtag ggtgtctaac ggtgggttgg gctaggccac
       61 acgcgaggaa cccaatgatg aagctaggca actgccatga ctaatatcct gccagtcgat
      121 tagccgggga catatggcga atcatgttgc ccgtgatcgg cgattcggga tcaagcgctc
      181 gcttttggtt ttgtcacggc gcggcttata ctgagaagat tgctgcgcgg gaaatcgaag
      241 tggactgaac cgagggagta agtgaagcca tgcgcaagca gtaagtcata gctacccaga
      301 gcacgattgt gccgccgagc attcgcagaa ctcctccgag aagatttgga gtattcctac
      361 atggacccgg gaacaacgaa attggttaat gtctcgaacc ctatagtacc gctcagtgta
      421 attaaaattc ctgcgaaaat cttgatcagc gcattcggga tatcctggaa gtaaattctt
      481 atggaagaga tcagccagac aaacgccgcc aggaaaggta aaccgcccac gaacatccat
      541 atccgggttt cggctaggag gtatacccca atcctaatga gacatcgatg aggataaaga
      601 caagaatgag aagtggtagg cgcggcagcc ggagtgtgca gcggaagctg caacagttaa
      661 gagctggcca tcaagtaaaa ccgctggggt aaaaaccccg gccaactacg tcttccaatg
      721 ctcactaaaa actcgcggac agtgtccatc tagctgcaag gcgcgcacac gccgaagaaa
      781 tcactaggcg actcagtgtg cataagcgaa gcctatcacg aatgacgaga aagcgaagga
      841 cagctaaatc agaaccaaca agtatgcggc agccaaaata aatccctaca acgatgaata
      901 ttatcagggt aacaagtaat gcgtcttcaa ggtagttcga ctggaagctg cggagcttgt
      961 tgatgtgagt atctccatcg acaccttctc cgatgattat tggacctcgc cggcgcagcg
     1021 cctgcgacag gtagtcaaag gaggcgggtt gtcgctgcgc agcgagggcg gggttattag
     1081 cacagcggcc gaggatagac caagcgcctc gtaaaattgc tgagtcggtg gcttttagat
     1141 ggttaagatg agcattctat ctcggactgc ggacctgatg atttcacaac aggggatttt
     1201 ttataccacc gcccgcccgg gtgtggtata aagtgcttcg gaatcatggc cgttctacac
     1261 atcacataca gacgcgaccg agaaacatct aacgccgatt acgacaattt ctacaaggtt
     1321 attacaagct accgctcccg cagactgtca acatcgcact ggacgatcca cacagaggag
     1381 ccgccagaat ctgtctggca gaaactcaaa tgccacatcg accccaatga ctatctgctg
     1441 atgctgccac tcgatccgag ttggttctcc ccaaaggatc ggaggctgct tagttggtta
     1501 gcggcgggac cgtaatcccg gtaaggagct gaggggcacg aactgcagtc cgatggataa
     1561 ataacagatg cttggaagtc tagtcgccgg tcttctcgtt ttcacatcag tgcttttggt
     1621 acggcgcggg cgaacaacca tctgccgccg gtgccgatcg cggattagac ggtggttctc
     1681 acaatgcccg gtctgcggtg cgaagcagga taactgagaa tgcccggcgc ggccgtaatt
     1741 acgagtcacg gcggagcccc gcagcagtag cggtaaggcg caccactaca aatttcgaaa
     1801 atacggccaa caagatcgcg aattaacgtt agacgagcta agcctccgga gaaccggatg
     1861 atcggccagc agtgcaggca ttgaagaatc agcaatacgt tctttcgacc ttgcttctgc
     1921 ttttcagtgt ttgggaaccg agatgtccct caaacggctg acaacattcg agacattcaa
     1981 aaggtcttaa acctttccct ttcaactccg tcgacgatta tagactgaat aaccagtcga
     2041 cttcttagtc tcatttagaa atccgagtcc tttcgtgtgg ccgcaacagc tctacgtgtt
     2101 tatttcaggc cggctcatca gatagtaatt cagcgagtga cgccagtaca atcaaaagcc
     2161 gcggcttacc ccgccatggc cggtccgcca attgggcgat cggtgtgtca gcccccatca
     2221 gctccatcgc cgcggtcgta ccaccgcaaa ccagctggct atcaatccgt cttctagaat
     2281 gcgcgggcat cttcgcgatc caggccagca gcgaatcgcc gctgatcacg cctcagccga
     2341 tattgtcttg gttaattgcg cgtgaacgga agtgcttcag ataaccatca gcacgagaga
     2401 tgtagttggg agagcaaagc gtgtgatgtt aatcattaag ttcctttatc taacgacgtc
     2461 acctcagggc accattccgg ctggaggcat gaaccggact ctacatcgag aatcctgatt
     2521 ccccgtgcgt gaaagccgta ccatggaaat cgtatactat cctggaaaat gcccggaatc
     2581 acagctaaac tatccgattc tgagatttga ttgacgaata ttgcctctac tcgggtttcc
     2641 ttaatgatta agattaaaca actatccaaa gcgtatttcg ctcgcgaact cttcaccgac
     2701 gttagtttac agatgaatgc cggcgatcgt ttgggtctgg tggggcgaaa cggtcacggg
     2761 aagtcgactc tgttcaaact tattttggga caggaagacc cggactcggg cgagatcacg
     2821 attccgcgca actatcgcat tggccatctc gagcaacatc tgcgctttac cgagccgacg
     2881 atcctggcgg aggccgccct gggattgccg gaagatgaat ctcattccct ctataaagcg
     2941 gaagcaatcc tgtttggcct gggattctcc gccgccgatc ttgagaaggc gccccgcgaa
     3001 ttttccgggg gcttccaaat ccgtatcaac ttggccaagc tcctggtttc cgagcccaac
     3061 ctgctgttgc ttgacgagcc gacaaattac ctcgacatca cctcggttcg gtggatcgcc
     3121 agattcctcg ccagcttcaa cggcgagcta attgtgattt cccacgatcg ggattttatg
     3181 gaccgcgtca cgacgcacac cgcggtgatc catcgccaga aaattcgcaa gttcgagggc
     3241 ggcaccgcca cggcttacgc acaaatcttc ttggaagacg aaatccacga aaagactcgg
     3301 gccaacaagg atagcaagcg cgcccacgcc gaagcgttta tcaaccgatt tcgcgcccag
     3361 gcttccaagg ccaagatggt tcagtcgcgc atcaagatgc tggaaaaaat gcccaagctg
     3421 gaccaactaa ccgagatcga atctttggat ttcgaattta gacatgcgcc gtttgcggcc
     3481 aaaacattgc tcgaagcgcg gggtatttca tttggttaca cgcgcgagca tcctcttttt
     3541 cagtacctcg atctgagcgt taataggcgc gaccgcatcg ctgtcatcgg caacaacggt
     3601 aaaggcaaat cgacgttgtt aaatgttttg tcaggcgggc ttcagccact gactggcgag
     3661 ctcaaagctc atcccgacat gaaactcgga tactttgggc aaaccaacat ctcgcggctc
     3721 aatccccaat tgaccatcga acaggagatc gagcacagca atccgaagct gacacgcacg
     3781 caggtccgta acatctgcgg cacgatgatg ttcggcggcg atctggcatt gaagaagatt
     3841 tcggtgttgt ccggcggcga gaaaagccgg accttattgg gcaagatcct ggctcaccct
     3901 tcgaatttac tcttgctgga cgaaccgaac aaccatctcg acatggaatc catcgatgcc
     3961 ctaatcgaga gcttacaaga ttttccaggc gcattattga tcgtcaccca taacgaacgg
     4021 attcttcgag ccctggctac gagattggtg gtgtttcagc gcggcagggt tgatgtcttc
     4081 aacatcggtt acggcgaatt cctaggaaag atcggctggg aagacgaaac agacggtaga
     4141 ggcgcgaaca aaccgacatc gcgaattaat tacaacgaga aaagagagcg cgaaaaagcc
     4201 gaacgaaggg a
//
LOCUS       DASUTG010000012         7070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_742593, whole genome shotgun sequence.
ACCESSION   DASUTG010000012 DASUTG010000000
VERSION     DASUTG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742593"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..1189)
                     /locus_tag="VF089_00245"
     CDS             complement(356..1189)
                     /locus_tag="VF089_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6172410.1"
                     /translation="MELDNHILEHISLYLDDELQNGERLGVEMHIKSCPGCRAALDAE
                     RELVCAIRCARPDYTTPASLQANIVQLVQSSGTRSKLGSSRPIIAACALLLLVVALFW
                     LAAPKSLQGDRSSFVTMAVNNHVRYLKGQLPLEVFSESPERISTWFAGKLGFDLKLPD
                     YPQGPAGPKPYHVEGGRLVGFKEDYAAYIVYRLQGHPISLVVTSDAVARPSGGEEILW
                     EGLRFHFQSVAGLKVLTWSDNGLTYALVSNLEERGQASCIVCHQDERVFRGLRSSENR
                     M"
     gene            complement(1176..1760)
                     /locus_tag="VF089_00250"
     CDS             complement(1176..1760)
                     /locus_tag="VF089_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6172411.1"
                     /translation="MIRFRKTASGSGEHAILENLSALYGFAMTLSRNRTDAEDLVQEA
                     CLRAIKGMTHTAIKSDPKVWLFTILRNVWINEWRRRANGPEFILLGKSRVDGGPLQPW
                     LSDERERPEDHFERDVTSGRIRLAISGLPEMFREVVVLRYFEGFSYRQIASILGCPAG
                     TVMSRLNRARAELRAVLDEKNNALVNFRRKRGTG"
     gene            2027..2782
                     /locus_tag="VF089_00255"
     CDS             2027..2782
                     /locus_tag="VF089_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX6172412.1"
                     /translation="MSHRGVFTFDFSKRVWLLLALAALVPSVAWSYEETKVTDGGTIE
                     GKVVFRGTVPSPRKVIPTQDQEVCGGPRDEPRLVLGSDNTIQGAVVYLKGVEKGKAFE
                     KPKKPPVVDNTKCKFEPELQVVPAGTEMLIVNSDPVLHNTHGYLLTDSRRGRTVFNQA
                     LPQKGQKVKSTLKSPGVVDVACDVHGWMQGWIVVADNPYFAVTEKNGTFAIKDIPPGK
                     YTLVTFQPFVGVRETAVMVEAKKTNSVNVELKK"
     gene            2827..3966
                     /locus_tag="VF089_00260"
     CDS             2827..3966
                     /locus_tag="VF089_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6172413.1"
                     /translation="MSDLERERREAARELNKPDDRPDPSLKITRRTFLHKSIVAGTVT
                     GVATYGWFPLINTLSMAFGAEAPFSFAWISDTHLYPKNVNTRFVEKATRAVKDVQAMK
                     PAADFLMFGGDLAQLADPVELRLGNDILKEITIKKHFIPGEHDWYLDMGKTWVSLFGN
                     SPWTFEHKGVTFIGVDTVSRAPDYWSATKMSPKERMGHMATLDGSVAGPWAGLGTDQL
                     QWLSKTLSPLPKNRPIVIFSHNPLYEYYPPWNFWVRDWRQVHEILKPYTNVTNIHGHT
                     HQVLYNEIGTMRSIGMLATSWPWPYAPEGVPALTKPMIRVNPGDHFDGVGWGKLTLSA
                     APKVDYEYMMWRKDVYAEAPIDAGTGDNDNQRLRPRLADQMWPYK"
     gene            3992..4450
                     /locus_tag="VF089_00265"
     CDS             3992..4450
                     /locus_tag="VF089_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172414.1"
                     /translation="MRKIVTWRGGLLLLAALLFIFISRMGDVQAHKEKHTPEQLKAFQ
                     DVFMEQVRIGDLLFHGDGEMEKKLGVNLSKTGMACAMCHPFASDTHPHEFPKFQEQIQ
                     EFATLRDMINWCIENPNEGERIDVNGPAMKALETYHYWSNKGSVLDAGKH"
     gene            4761..5537
                     /locus_tag="VF089_00270"
     CDS             4761..5537
                     /locus_tag="VF089_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX6172415.1"
                     /translation="MLAEIFTGLKNRLVLGLEPSATEEILREIHTPKLILPAMTRNGN
                     HVPVVVKMNHPMESSHYIRRVEFRNESDPIPSKGIFYPSAANGETYLAFQARMDSGIS
                     TVLAIAECNVHGTWTARRRITIPDGQGGCATVGVPKEAAAREAILPPVIRIPELVRRG
                     RLTRGETAEVQVKFKHPSKTGLAYKDKKFVQVEEPLYVTSMQVLYGESLVSRYQMTAG
                     LSDDPFLKFKLKFIEEKPIRIVFTNSSGRQFSASTAVVLS"
     gene            5558..6400
                     /locus_tag="VF089_00275"
     CDS             5558..6400
                     /locus_tag="VF089_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX6172416.1"
                     /translation="MTASVILIGLLWKIAPGKTLPEMLGLTGAESVVASRAELAAGFH
                     NSPLPAAQCGSCHQQHYNQWRRSFHARSLTSDDFVRTFSQYLQSIGEQAQEDPQSSMA
                     CLSCHAPLLKHAAPETIRQVGGFVLTRETKKLDGFEVGCVACHGAASRVFSGSIEKPQ
                     DNPFHLSTFSPSYKEASFCADCHTWTSPKIPCSNVYTDWKISRAAKRGATCQSCHMAE
                     TSGSAAAGGPERKIHDHIFPGGRSAAMLRKAVELGLKADFRKDRLEVTATIRNLTPHR
                     VPDG"
     gene            6428..6754
                     /locus_tag="VF089_00280"
     CDS             6428..6754
                     /locus_tag="VF089_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172417.1"
                     /translation="MTVSDETGWEFENNKRIYANFGLDANGETTGQAWKILKQSPEST
                     ALKAEEIRIEKMSFNVEPRDGKRFTIQARMRYQYAPAQQDGSDQELDAARMAEATLTL
                     PGKRPS"
     gene            6773..>7070
                     /locus_tag="VF089_00285"
     CDS             6773..>7070
                     /locus_tag="VF089_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6172418.1"
                     /translation="MADHVIEKGNEVINPELIKGWLLWGFVWVLLAPTLGVIISTKFT
                     YPEFLEGVPALTFGRLRPIHVNGVIFGTFSTLFIGLCYYIVPKLAQVRVYKEEWG"
BASE COUNT         1717 a         1878 c         1942 g         1533 t
ORIGIN      
        1 gaggccttga cggtcgcgaa gccgacggcc acggcataaa gatagcgcat gccgcgcgtt
       61 gattccgctg cggctcgagc atgatttagc gaagggaaag ctcaaagggg agctgttcgc
      121 tgagccggaa tctgccttac gggtgcgctg ggcaaactgg cataaagact tttctgataa
      181 acaagcctgg ccgttaggtc ctaggaagtt tgattctacc gacagacttt gatgaacaaa
      241 ctgtttgcgc tgccttcatg tgagttagga atccaacgtt ctgagaagaa ggcagtctct
      301 aacggggagg gttcatctta tgccggtgag atgcgcgagc cgaatcgctc aatcttcaca
      361 tgcgattttc ggaagatctg agacctctaa aaacccgctc gtcctggtgg catacgatac
      421 aggaagcttg cccgcgctct tccaaattgg aaacgagagc gtacgtcaat ccattgtcag
      481 accaagtcag cactttcaaa ccggctacgg attgaaaatg aaaccgcagt ccttcccaaa
      541 ggatctcttc accacctgat ggtcgcgcca ccgcgtctga cgtcaccacc aaagagatcg
      601 ggtgaccctg caagcgatag acaatatacg ctgcatagtc ttctttgaaa ccgaccaaac
      661 gtccgccctc gacatggtaa ggcttcggtc ccgcgggtcc ctgcgggtaa tccggcagtt
      721 tcaaatcgaa gccgagcttt ccggcgaacc aggtggagat cctctcggga gactcgctaa
      781 atacttccag gggtaattgt cccttgagat aacgcacatg attgttgacg gccatggtaa
      841 caaatgagct gcggtctcct tgtaatgatt ttggcgcggc caaccagaaa agcgccacaa
      901 caagcagcaa tagggcgcag gctgctatga tcggccgtga agatccaagc ttggatcgcg
      961 tgcctgagct ctgcaccagc tgcacaatgt tcgcctgcaa tgaggccggc gtcgtataat
     1021 cgggacgcgc gcagcgtatg gcgcaaacaa gctcacgttc ggcgtccaga gcggcgcggc
     1081 atccggggca gctcttgatg tgcatttcca cacccaggcg ctcgccgttt tggagctcgt
     1141 catccagata caacgagata tgttctaaaa tgtgattatc cagttccacg ttttcgccgg
     1201 aaattgacta aggcattgtt tttctcatcc agtacagcac gaagttccgc ccgcgcgcga
     1261 ttcaaacggg acatgacagt tccggcagga catcccaaga tggaagcgat ttgccggtag
     1321 ctaaatcctt caaaataacg gagcaccacg acttcgcgaa acatttctgg caaacctgaa
     1381 attgccagac gaatcctgcc ggatgtaacg tcgcgctcaa aatgatcttc cgggcgctct
     1441 cgctcatcgg acaaccatgg ttgcagcgga ccgccgtcga cgcgagattt tcccagcaga
     1501 ataaactccg gcccgttcgc acggcggcgc cattcattga tccagacgtt gcgtaggata
     1561 gtaaacagcc acactttggg atcgctctta atcgcggtgt gggtcattcc tttgatggcg
     1621 cggaggcagg cctcttgcac taagtcttcg gcgtccgtgc gattgcgact caaggtcatc
     1681 gcaaatccat agagcgcgct gaggttttcg agaatagcgt gctcgccgct gccagacgct
     1741 gttttccgga atcggatcac agcagatact cctcgcaaga gatccgttgc gacttcggtt
     1801 tgtcaaaact cgatcacgct gcttaattaa acctctctcg ttttcatgtc aatcacaaac
     1861 cgcgacttgc gcgggagaaa gaaacgtcgg agaactgagc ctcgtgattt atcgctccgg
     1921 accgcaaaac ccgggaataa atttcttcct cggttgtctt tatctaacag cagctcaaaa
     1981 cagttctggc cgttagatcg acccgcttga tcatgggagg caaaccatgt cccatcgcgg
     2041 tgtatttacc ttcgattttt ctaaacgcgt ttggctcttg ctcgcgcttg cggctttagt
     2101 cccttctgtc gcctggtcgt acgaggagac aaaggttacc gacggcggca cgatcgaagg
     2161 aaaggtcgtg ttccgcggca ccgtcccgag cccccggaag gtaattccga ctcaagatca
     2221 agaagtttgc ggcgggccaa gagacgagcc gcggctcgtt cttggatccg ataacaccat
     2281 tcaaggagcc gttgtttact tgaagggcgt cgaaaagggc aaggcgttcg aaaagcccaa
     2341 aaaacctccg gttgttgata acacgaagtg taagttcgag cccgagctgc aggtcgtgcc
     2401 ggccgggacg gaaatgctca ttgtcaactc cgatcctgtg cttcacaaca cccatggcta
     2461 tctcttgacg gattcccgcc ggggacgcac ggtttttaac caggccttgc cgcagaaggg
     2521 tcagaaagtg aagtcgaccc ttaaatctcc gggggtagtg gatgtcgcct gcgacgtcca
     2581 cggttggatg cagggctgga tcgtggtggc ggacaacccg tatttcgcgg tcaccgaaaa
     2641 aaacggcacg tttgcgatca aagacatccc gccgggaaaa tacacgttgg ttacttttca
     2701 accttttgtc ggagtcagag aaactgctgt gatggttgag gcaaagaaaa ccaacagcgt
     2761 taacgtcgag ctaaaaaaat agcggcaaag ccgccaagcg attcctgttt caggaaggag
     2821 aaagcgatga gcgatctcga gcgtgaacgg cgcgaggctg cgcgcgagct gaacaaacct
     2881 gacgaccggc ccgatccatc tctcaagata acccggcgga cttttctcca taagtcgatc
     2941 gtggccggga ccgtgacggg ggtagctacg tacggatggt ttccgttgat caataccctg
     3001 agtatggcgt tcggagctga ggccccattt agcttcgcct ggatttcgga tacacacctg
     3061 tacccaaaaa acgtcaatac gcggtttgtc gagaaggcaa cccgcgcggt taaagacgtt
     3121 caggcgatga agccggcggc cgatttcctc atgttcggcg gcgacctggc gcaactcgcc
     3181 gaccctgttg agctgcggct gggaaacgac atcctcaagg aaatcacgat caaaaagcat
     3241 tttatcccag gggaacacga ctggtatctc gacatgggca aaacatgggt gagcttattc
     3301 ggcaactcgc cttggacctt tgagcacaaa ggcgtcacat tcattggcgt cgatacggtg
     3361 agccgcgctc ccgattattg gtccgcgacc aagatgagcc ctaaggaaag aatggggcac
     3421 atggcgacac tggatggatc ggtggccgga ccatgggcgg ggctcggaac cgatcagctt
     3481 cagtggttga gtaaaacgct ttcccctttg cccaaaaacc gacctatcgt gattttcagt
     3541 cacaatccgc tctacgagta ttatccgcca tggaacttct gggtccgcga ttggcggcaa
     3601 gtgcacgaga tactcaagcc ctatactaac gtcacgaata ttcacggtca tacgcatcag
     3661 gttctctata acgaaatcgg cacgatgaga tcgatcggga tgctagcgac ctcttggcca
     3721 tggccctatg cgcctgaagg agtgcccgcg ctgaccaagc cgatgatccg ggtcaatcct
     3781 ggagaccact ttgacggggt aggctggggg aaactgacgt tgagcgcggc accaaaggtg
     3841 gattacgaat atatgatgtg gcgtaaggac gtttacgccg aagcgcccat cgatgccggt
     3901 accggcgaca acgacaacca gcggctcaga ccgcgcctag cggatcagat gtggccgtat
     3961 aaatagccga cgaaaggaaa ggtgcatcgt tatgagaaag attgtcactt ggcgtggtgg
     4021 acttcttctc ctggcagcgc tccttttcat tttcatttct cgaatgggtg atgtccaggc
     4081 gcataaagaa aagcatacgc cggaacaact caaggcgttc caggacgttt ttatggaaca
     4141 ggtgcggatc ggagatctgt tgtttcacgg cgacggcgaa atggaaaaaa aactcggcgt
     4201 gaatttatcc aaaaccggca tggcgtgcgc gatgtgtcac cctttcgcgt cggacacgca
     4261 cccccatgag ttccctaagt tccaggaaca gattcaagag tttgcgacct tgcgggacat
     4321 gatcaattgg tgcatcgaaa atcccaacga aggcgaaaga atcgatgtca acggtcccgc
     4381 gatgaaagca ctggagacgt atcattactg gtccaacaag ggttcggtct tggatgccgg
     4441 taagcactag cagccagctg aaaaagactc gctcgatttc gtgttcgtgg ctcgtgttag
     4501 cgcgcgcaga tttcagttta agtttcatga ccgatcacga ggaaggcgcg ccttgccggt
     4561 aagatctttt agccggccgc aatcagattt tttcagtgct gctaggaaaa cccttccttc
     4621 taggaagggt gaggggtgaa taccgatcct tgatggccga caatcaaaat tggcaagtat
     4681 gctgcgccgc gcgctaagtg tatgagatga gccggttgga aatttcgcga agaaactttt
     4741 gcaaatggag ctcttcactt gtgctggcgg aaatctttac gggtctgaag aaccggttag
     4801 tgctggggct cgagccctct gcaacggagg aaatcctacg agagatccac acgccgaagc
     4861 tcatcctgcc ggcaatgaca cgaaatggaa atcacgttcc tgtcgtcgtc aagatgaacc
     4921 atccgatgga atcgagccat tacatccggc gagttgagtt ccgtaatgaa agcgacccga
     4981 ttccatccaa aggaattttc taccccagcg ccgctaacgg tgagacctac ttggctttcc
     5041 aagcccgaat ggacagcggc atctcaacag tcctcgctat cgccgaatgc aatgtgcacg
     5101 gcacttggac cgcccggcgc cgaattacca ttcccgatgg gcaaggcggc tgcgctacgg
     5161 tcggcgtgcc gaaagaagct gcggcaagag aagcgattct cccgcccgtg atcaggattc
     5221 ctgagctagt tcgcaggggc cgtttaacca gaggagaaac ggccgaagtt caggtaaagt
     5281 tcaaacaccc aagcaagaca ggtctcgctt ataaagataa gaaattcgtt caggtcgaag
     5341 agcctctcta tgtgacatcg atgcaggttc tgtacggaga gagtttggtc agccgctacc
     5401 aaatgaccgc aggtttgagc gacgatccgt ttttgaaatt caagcttaag ttcatagaag
     5461 aaaaacctat tcggattgtc tttaccaata gtagtggccg gcaattcagc gccagcacgg
     5521 cagtcgttct atcttaaact tcatgggccg ggtctcgttg acagcgtcag ttatcctgat
     5581 cggcctactt tggaagatcg ctccaggaaa gaccttaccg gaaatgttgg gattgacagg
     5641 agcggaaagt gttgttgcga gccgcgcaga gctggccgcg ggctttcata acagcccgct
     5701 tccagctgct cagtgcggct cttgccacca gcaacattat aaccagtggc gccgctcatt
     5761 tcacgcgcgc tctctgacgt ccgatgattt tgtcagaaca ttctcacagt atctccagtc
     5821 gatcggagag caggcgcaag aagatccgca atcgtcgatg gcgtgtctct cgtgccatgc
     5881 gcctcttctg aaacatgccg cgccggagac cattcgccag gtcggcgggt ttgtgctgac
     5941 ccgggaaaca aaaaaactcg atggcttcga agtcggttgt gtcgcttgtc acggcgctgc
     6001 aagccgggtg ttttccggat cgatcgaaaa gccgcaggac aatcccttcc atctttcaac
     6061 attttccccg tcctacaaag aagcttcatt ctgcgccgac tgccacacgt ggacgtcacc
     6121 taagattccc tgctccaacg tctacacgga ctggaagata agccgggcgg caaaaagagg
     6181 cgcaacctgc cagtcctgtc acatggcgga gaccagcggg agcgccgctg ccggaggacc
     6241 ggagagaaaa atacacgacc atatttttcc tggcggacgt tcggccgcca tgctgcgaaa
     6301 agcagtagag cttggtctga aagccgactt tcgcaaggat cgtctcgaag tgaccgccac
     6361 gatccgcaat ctaactcctc atcgggttcc cgatggctga ccatggaaca gctccgcggt
     6421 cctggaggtg accgtatcgg atgaaaccgg atgggaattc gagaataaca agcgcatcta
     6481 tgccaacttc ggcctcgatg cgaatgggga gaccactggc caggcctgga aaattctgaa
     6541 acagtcgccg gagtcaaccg cccttaaggc agaggaaatt cgcattgaaa aaatgagttt
     6601 taacgtcgag ccgcgagacg ggaagcgctt cacgatccaa gcgaggatgc gctaccaata
     6661 cgcccccgcg caacaggatg gctccgacca agagctcgac gcagcgagaa tggcggaggc
     6721 aactttaaca ctaccgggta agcgcccgtc ttagtttgca agagaagatg gcatggcaga
     6781 tcacgtgatt gaaaagggca atgaggtgat caacccagag ctgatcaaag gttggctcct
     6841 ctgggggttc gtctgggtgc ttttagcgcc gaccctgggt gttatcatct cgaccaagtt
     6901 cacctatccc gagttcttag agggagtccc ggcgttgacg ttcggcaggt tgcggccgat
     6961 ccacgtcaat ggcgtgatct tcggaacttt ctcgactctc ttcatcggcc tttgctatta
     7021 cattgtgccg aagctggcgc aggtccgagt ctataaagag gaatggggct
//
LOCUS       DASUTG010000013         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1556, whole genome shotgun sequence.
ACCESSION   DASUTG010000013 DASUTG010000000
VERSION     DASUTG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF089_00290"
     CDS             complement(<1..847)
                     /locus_tag="VF089_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX6172419.1"
                     /translation="MRIHHPTAVGFLLVLLSLLLGYHPAVGQQHRALSQQLVGGEFSY
                     TAKRGDSLIGIGARFGVDAAVIAADNNLSASSPVKFGHVLRINNRHIIPKRIEEGILI
                     NIPQRLLFHFKEGRLLRFFPVGLGRRDWPTPTGHFNIVVKEENPTWDVPKSIQDEMQR
                     EGKTVKTCVPPGPDNPLGSHWLGLSIPGYGIHGTIAPTSIYRFQTHGCIRARADDIAQ
                     LFGEVSRGTPVIVIYERLLIAKIGQQVYLEAHRDVYKKDSDAPRLFEELLQAFNLESV
                     VDRQLA"
     gene            complement(899..1612)
                     /locus_tag="VF089_00295"
     CDS             complement(899..1612)
                     /locus_tag="VF089_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6172420.1"
                     /translation="MPEPVAQRCFHCQKNRPYESMIPGEMLRPAIIETIKKRDTSWAP
                     SRMICVDCLDLFRSEYVEDALKDEVGELSKLEQEVVDSLREQENLTENINRAFEKSIT
                     FGQHVADRVASFGGSWIFIGAFFLVLAAWIGINSVATIRTPFDPYPYILLNLILSCLA
                     AIQAPIIMMSQNRIEVRDRLRSENDYQVNLKAELEIRHLHEKLDVLLKHQWQKLLEIQ
                     QIQMDFMKELVRRNPDPRN"
     gene            complement(1631..>2857)
                     /locus_tag="VF089_00300"
     CDS             complement(1631..>2857)
                     /locus_tag="VF089_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6172421.1"
                     /translation="PEHKLRIVSLLKEMGEIVAVTGDGVNDAPALKKADIGIAMGMRG
                     NDVAKEASRMILTDDNFSSIVAAIEEGRAIFDNIKRFAAYVLNSNPQEMYPSIFWVLW
                     PGTPLAMTIMGVLAVDVGTDLIPAMGLGIEPPEKGIMERTPRPRDEKLLSLKFILRSY
                     FVQGTLLALSCYATYFYMGWVLGAWEPGQSIASMPPSPADLDFGEASSAYLQTLTAYF
                     FPTVTAQIANVLCKRSWQSSLFSWNFLHPLRRSEALAAIANWRPPRYTARVHIEYHVQ
                     RAAEFTAVRALFALIGGLVLLPFRFIWMLLSQSLVRLERPVIAPFTRSVAAFFERHYI
                     IFNFISNPLIDLGILFELALCYVFFYTPLAEIYYFAPLPWHVYLFAFHGTVLLIVFEE
                     AKKYYRRKGYRLEFLG"
BASE COUNT          698 a          729 c          792 g          638 t
ORIGIN      
        1 gtgccagctg acggtcaacg acagattcaa gattgaaggc ttgcagcaat tcttcgaaaa
       61 gtcgtggggc atctgaatct tttttgtaaa catctcgatg ggcttctaag taaacttgct
      121 gcccgatttt ggcgatcagg agtcgctcat agatgactat aacgggagtg ccgcgcgaca
      181 cctcgccaaa aagctgggcg atatcgtccg cgcgggcgcg tatgcagcca tgggtttgaa
      241 accgatagat gcttgtcggc gcaatggtgc catgtatgcc atagcccggg atactaagcc
      301 ccaaccagtg acttcccaaa ggattgtccg gacccggcgg aacacaagtc ttcaccgtct
      361 tgccctcacg ctgcatctca tcttgaatcg attttggcac atcccatgtg ggattttcct
      421 cttttacgac gatgttgaag tgaccggtgg gcgtcggcca gtcccgcctt cccagcccca
      481 ccggaaagaa acgaagcaac cggccttcct taaaatgaaa aagcagacgt tggggaatat
      541 tgatgaggat accctcttcg atccgtttgg gtatgatgtg acggttattg atgcgcagca
      601 cgtgtccgaa ttttacagga gagctcgcag aaagattatt atctgcagcg attaccgccg
      661 catctacacc gaatcttgcg ccgatgccga tcagcgaatc tcctcgcttg gccgtataag
      721 aaaattcgcc gccgacgagt tgctgcgata acgcccggtg ctgttggccg acagccgggt
      781 gatacccgag aagcaacgat aacagcacta gcaaaaaacc gacggcggtg ggatggtgta
      841 ttcgcatttc aatgcttccg tgttctgaag tgccatgcag catcggctga ttgttcagtc
      901 aatttctggg atccggattt cgtcgcacca gctctttcat gaaatccatc tgaatttgct
      961 ggatctctaa aagcttttgc cactggtgct tgagcaagac gtcgagtttt tcatgcaagt
     1021 ggcggatctc aagctcggcc ttgagattca cttggtagtc gttttccgag cgcagacggt
     1081 ctctgacctc gatgcggttt tggctcatca tgatgatcgg tgcctgaata gccgcgaggc
     1141 aagacaaaat caaattcaac agtatatacg ggtagggatc gaaaggggtg cggatggtgg
     1201 caacgctgtt gatgccgatc catgccgcca gcaccaaaaa aaaagcgcca ataaaaatcc
     1261 aactgccgcc gaaggacgcg acgcgatcgg cgacatgttg accgaacgtg atactctttt
     1321 cgaaggcgcg gttaatattc tccgtcaagt tttcctgctc tctcagacta tcgacgacct
     1381 cctgttctaa tttgctgagc tcacccacct cgtctttgag ggcgtcttcc acgtattccg
     1441 acctgaagag atcaaggcag tcgacgcaaa tcatccggct aggggcccag ctggtgtcac
     1501 gcttcttgat cgtctcaata atagccggcc taagcatctc gccaggaatc atggattcat
     1561 aagggcgatt tttctgacag tgaaaacagc gttgtgcgac gggttctggc ataattcctc
     1621 cttcaggcga ttagccgaga aattctaacc tatatccctt tcgccggtag tatttcttgg
     1681 cttcttcaaa gacgatgagc agcaccgtgc cgtggaacgc gaataagtaa acatgccagg
     1741 ggaggggggc gaagtagtag atctcggcaa gcggcgtgta gaaaaaaaca tagcaaagcg
     1801 ccagctcgaa caagatgccg agatcgatga gcgggttgga gatgaaattg aagatgatgt
     1861 agtgccgctc gaaaaaagcc gcaaccgatc gggtgaacgg cgcgatgacg gggcgctcaa
     1921 gtcgcacgag ggattgagat agcagcatcc agatgaatct gaagggcaga agcactagcc
     1981 cgccgatcag agcgaagaga gcgcggacgg cggtgaattc ggcggcgcgt tgaacgtgat
     2041 actcgatgtg cactcgcgcg gtgtaccgag gcggccgcca gtttgctatg gctgccagcg
     2101 cttcactgcg acgaagcgga tggagaaaat tccaagagaa tagtgagctt tgccatgagc
     2161 gtttgcataa tacattggca atctgcgcgg ttacggtggg gaaaaaatat gcggtcaatg
     2221 tttgcagata agccgacgac gcttcaccga agtcgaggtc tgctggcgac ggcggcatcg
     2281 aggcaatact ctggcccggt tcccaggcgc cgagcaccca acccatataa aagtaagtag
     2341 cgtagcagct aagcgcgagc agagttccct ggacaaaata agagcggagt atgaacttga
     2401 gcgaaagcag cttttcgtcg cggggccgtg gcgttcgctc catgataccc ttttcgggag
     2461 gctcgatgcc taagcccatc gccggaatga ggtccgtgcc cacatccacg gccagaactc
     2521 ccatgatcgt catggccagg ggtgtgccag gccacagaac ccagaagata gagggataca
     2581 tctcctgagg attactgttg aggacgtagg cggcgaaacg tttgatgtta tcgaagatcg
     2641 cgcggccctc ttcgatggcg gcgacaatgg agctgaagtt atcgtcggtc aaaatcatgc
     2701 gcgaggcctc tttggcgaca tcgttaccgc gcattcccat agcaatgccg atatcggctt
     2761 tcttcaaagc gggtgcatca ttaacgccat cgccggtgac cgccacgatc tcgcccatct
     2821 ctttcagcaa cgaaacgatg cgaagtttat gttccgg
//
LOCUS       DASUTG010000014         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_446348, whole genome shotgun sequence.
ACCESSION   DASUTG010000014 DASUTG010000000
VERSION     DASUTG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446348"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VF089_00305"
     CDS             complement(<1..542)
                     /locus_tag="VF089_00305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX6172422.1"
                     /translation="MARFFLPARQIEGHRATISGVELNHLRRVLRLRTGDRVVVFDDA
                     GCEHEGIIRSLSEDRGEIEIIRSYQVNNESPLKTILALGLTKGEKMDWVVEKATELGV
                     NSVVPFVSSYTIPKLDEKKVAQRSERWQKIALSAAKQCGRVRIPEILAVTAFRDLVEP
                     VSRDALRLLFWEKECEQGLS"
     gene            complement(546..1412)
                     /locus_tag="VF089_00310"
     CDS             complement(546..1412)
                     /locus_tag="VF089_00310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6172423.1"
                     /translation="MTSSWLQLSLRTDPSALDAVSNFLIERGSPGVVINKNQVQAYFA
                     CAEDTATIRRDVQRFLRGIRGMYPEIGEDQLRWRVLKDRNWNYSWRRFFSPQKVGQVF
                     LVAPPWAKLPPLGRRHLIVIEPGMAFGTGTHATTRGCMEFIERIARSLGTEPFTALDV
                     GTGSGILAIALAKVGASKVWALDNDPVALKVARENIACNGVEKIVSCSASPLNRLRTF
                     GVVVANLTAETITALAEPLVKRVAPAGYLILSGILTPKSDQLVRHFDSRGFRLIKLKK
                     EKEWATMLLKRK"
     gene            1641..2531
                     /locus_tag="VF089_00315"
     CDS             1641..2531
                     /locus_tag="VF089_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6172424.1"
                     /translation="MKLASIKPNDIGLVKNDSLILIGETLARESALPKGCSMLEVIAN
                     YASVNDLLESYAAKITATKLDPKMLKPPIEKPSKIWAAAANYKRGTGGLDDARGRGAA
                     GTATAEQLLERIFLKPPSCICGPEDNIIIPNGAETIFPELELCVVIGKETRNISKERA
                     YEAIFGYTILLDVTARGYGSGKDLSGSRCVRKGFETFAPIGPWITTKDEIADPHQLSM
                     KLWVNGELKQSARTDSMVNDIPTLVSYLSQVTTLFPGDLISTGNPDAPEFQEKLQPGD
                     IVKSEIEGIGSMNLGVAKQS"
     gene            2609..>3337
                     /locus_tag="VF089_00320"
     CDS             2609..>3337
                     /locus_tag="VF089_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX6172425.1"
                     /translation="MVRIAPFRGLFYNQKKIRDLARVVTPPYDVISPEEQEKLYRKSP
                     YNFVRLDFNPEPDPYVAVAELFANWQAEGIFERDEEPAIYFAVHKFALKGGEIKERQG
                     FYALIQLQDFSSGEIRPHEKTLQAPKNDRFQLMLASHAQLSSIFALYSQPKDKINRIL
                     SEQTEGKAPFIEVKLDSGDECRLWRITDPALIREIQRQMKEQHLVIADGHHRYEATLR
                     YRDQMRRDGSPTGRESFNYILTYFA"
BASE COUNT          812 a          933 c          863 g          729 t
ORIGIN      
        1 tcagacaatc cttgttcgca ctccttctcc cagaataaca gccgcagcgc atcgcgcgat
       61 accggctcga cgagatcgcg gaaagcagtc acagccagta tctccgggat ccgaacgcgg
      121 ccgcactgtt ttgccgcgct cagtgcaatc ttctgccagc gttcggaacg ctgcgccacc
      181 tttttttcgt ccagctttgg tatcgtgtaa ctggaaacga atgggaccac gctgttaacg
      241 cctagctcgg ttgccttttc caccacccaa tccattttct cacccttggt gagacctaga
      301 gcaaggatgg ttttcagcgg cgattcattg ttgacctgat aagagcgaat tatctcgatc
      361 tcgccgcgat cttcgctgag cgagcgaatg attccttcgt gctcgcagcc ggcatcgtcg
      421 aacacgacaa cacggtctcc cgttcgcaag cgcaacactc gccgaaggtg atttaattcc
      481 acaccgctga tggtagcccg atgaccctct atctgccgcg ccggcaggaa aaaacgcgcc
      541 atggcctact tccgcttcag aagcatagtc gcccattctt tctctttctt gagcttgatc
      601 agcctgaagc cgcgcgagtc aaagtggcgc accagctggt ctgacttcgg cgtcaagatg
      661 ccggacagaa tcaagtagcc agcgggagca acgcgtttta ccagcggctc agccaacgcc
      721 gtaatcgttt cggcagtaag gttggcgacg acgacgccaa aggttcttag acggttgagc
      781 gggctcgcgg aacacgagac aatcttttct accccgttgc acgcgatgtt ttctcgggcg
      841 actttcaatg cgaccggatc gttgtctaag gcccacacct tactcgcgcc cactttggca
      901 agggcgatcg ccaaaatccc ggaaccggtg ccgacatcaa gagctgtgaa tggttctgtg
      961 cccagagaac gagcgatgcg ctcgataaat tccatgcagc ctctcgtggt ggcgtgagta
     1021 cccgttccga aagccatccc cggctcgatg acgatcagat gccggcgacc tagcggcggc
     1081 aacttggccc acggaggggc gaccaagaat acctgcccta ctttctgtgg tgaaaaaaat
     1141 cgccgccacg agtaattcca attccggtct ttgagaacgc gccagcgcag ctgatcctcg
     1201 ccgatctccg gatacatgcc gcgaataccg cgaaggaacc gctgcacatc ccggcgaata
     1261 gttgccgtat cttcggcaca tgcaaagtat gcctgcactt ggttcttatt gatgacaact
     1321 cccggagatc cccgttctat gagaaagttc gataccgcgt ctagtgccga tgggtcagtg
     1381 cgcaaagaga gctgcaacca ggaagatgtc acaccttttt atgccataga gttggcagtc
     1441 gggcaaatta tgtgaacgtt gaggtgacgc gctccatcgc cgcattcccg agactagtgc
     1501 agaattgacg gcgatcgccc ccctacatga tccatcatga tcaagtctct ccctgtgcag
     1561 ggaccgacag ctttcattgg cgccttgaac agttccgcag cacgatatat ggacaaggtt
     1621 acgatgaaag gagcgacgag atgaagttgg ccagcatcaa accgaatgac attggtcttg
     1681 taaaaaacga ttcgctgatt ttaatcggcg aaacgttagc tcgtgaaagc gccttgccca
     1741 agggctgctc gatgctcgag gtgattgcta actacgctag cgtcaacgat ttgctggaga
     1801 gttatgcggc caagattacg gcgaccaaac tggacccgaa gatgctgaaa ccgccgatcg
     1861 agaaaccctc taagatctgg gccgctgcgg ccaattacaa acgcggtacc ggcggcttgg
     1921 atgatgcgcg cggcaggggc gccgccggca cagcgaccgc tgaacagctc ctcgaacgaa
     1981 tttttctaaa acctccatca tgcatttgcg ggccggaaga caacattatt attcctaatg
     2041 gagcggaaac catttttccc gaactggaac tttgtgtcgt gattggcaaa gaaacccgga
     2101 atatttcgaa agagcgtgcc tacgaggcaa tttttggcta tacgattctg ctcgatgtta
     2161 ccgcgcgcgg ctacggctcc ggcaaggacc tgtcaggcag ccgttgtgtg cgcaaaggtt
     2221 tcgaaacgtt tgcaccgatt ggaccttgga tcacgaccaa agacgaaatc gccgatccgc
     2281 atcaactctc aatgaagctg tgggtcaatg gagagctcaa gcaatctgcg cggactgatt
     2341 caatggtcaa tgatataccg actctggtca gctatttgtc acaagtgacc acgctttttc
     2401 ctggcgacct gattagcacg ggcaatcccg atgcgccgga attccaggag aagcttcagc
     2461 cgggcgatat cgtcaaatcg gaaatcgaag gtatcggctc gatgaacctc ggtgttgcga
     2521 aacagagttg agcccgcgac cagaactcca acaagtcttg atcgaaccca agttcaagat
     2581 tttcaactta agctagtaag cagggtacat ggttcgaatt gcaccctttc gcggactttt
     2641 ctacaaccaa aaaaagatcc gcgaccttgc acgggttgtc actcctccgt acgacgtgat
     2701 ttcaccggaa gagcaagaaa agctttatcg caaatcgccc tacaacttcg tccggttaga
     2761 ctttaatcct gagccggatc catacgtcgc agtggcggaa ctatttgcaa actggcaagc
     2821 ggaagggatt tttgagcgcg atgaagaacc ggcaatttac tttgccgtcc acaagttcgc
     2881 gttgaagggc ggagagatca aggaacgtca aggtttctac gccttgatcc aactgcaaga
     2941 cttctcgagt ggtgagatcc gtccgcacga aaagaccttg caagctccga agaatgacag
     3001 gttccaactc atgcttgcat ctcacgccca attgagctcg atcttcgcgc tttactccca
     3061 gccgaaagac aagatcaatc gcattttgtc cgaacagaca gaaggcaaag cgcctttcat
     3121 cgaggttaag ctggacagtg gcgatgagtg ccgcctctgg cgcatcacag atccggcatt
     3181 gatacgcgag atccagcgcc aaatgaagga acaacatttg gtgatcgccg atggccatca
     3241 ccggtatgag gcgacgctca gataccgcga tcagatgcgc agggacggct caccgaccgg
     3301 ccgcgaatcg ttcaactaca tcctgactta tttcgcc
//
LOCUS       DASUTG010000015         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1039364, whole genome shotgun sequence.
ACCESSION   DASUTG010000015 DASUTG010000000
VERSION     DASUTG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039364"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /gene="lhgO"
                     /locus_tag="VF089_00325"
     CDS             <1..950
                     /gene="lhgO"
                     /locus_tag="VF089_00325"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HEX6172426.1"
                     /translation="VAMTREELPRLEELHRRGEANGLRGLQLIDTGRLNELEPHATGI
                     KGLYVPETGIIDYKKVAAAYAGKIRDSGGDIRLSQKVVGILDRPEEIVLQTSGGDYRT
                     KHLINCCGLQSDLIAQMTRSADQDGGLEHRIIPFRGEYYKITPEREFLVKNLIYPVPD
                     PTFPFLGVHFTRMAKGGVEAGPNAVLAYAREGYSHSQFNLNDLWRTISFKGFWAMTGK
                     YWQTGFGELYRSLNKAAFVRALQKLVPDIRENDLVPGGAGVRAQAVSANGALVDDFVI
                     KQGRNAIHVLNAPSPGATASLAIGRQICEMAEKNFRLRN"
     gene            complement(1022..1273)
                     /locus_tag="VF089_00330"
     CDS             complement(1022..1273)
                     /locus_tag="VF089_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172427.1"
                     /translation="MVSSAEYSATIFDEEVEIKVGSECKALHSYTVGQAGKISSSSYD
                     GRSFYPPRNYSDGFHLDRTPTFPGCDRFAANPHLYSATV"
     gene            1278..1805
                     /locus_tag="VF089_00335"
     CDS             1278..1805
                     /locus_tag="VF089_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX6172428.1"
                     /translation="MIIEYKGKKPAVSPQAFVAPTAVLIGDVSVGADASIWWGAVLRS
                     DQGGFPIVIGARTSIQDNCVIHSGEASTIIEEDCTVGHGAIMEGCVIKRGAIIGMNST
                     ILENTEIGEESLIAAGSVVVPHTKIPPHVLAAGTPAKVKKAIEGEALKWIKMGASTYV
                     ELCRSYLGGDYKIVG"
     gene            1839..>2494
                     /locus_tag="VF089_00340"
     CDS             1839..>2494
                     /locus_tag="VF089_00340"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6172429.1"
                     /translation="MRIILIGQAAFAEKTLDKLVKKGEEVLAVFCFPDAPGGKIDPVK
                     QRALQLGIPVHQHKTLKTPEVTEQFVSLKADLAILAFVSQIIPPPVFNAPRFGSICYH
                     PSLLPKYRGRTAINWALIRGETETGLTIFWLDKGIDTGPILLQKEVRVDPDDTTGTLY
                     FNKLFPLGIEAIGEAVDLIKAGNPPRIVQDESKATYDPPCGDEHAKIDWSKPAQEVYN
                     "
BASE COUNT          599 a          658 c          700 g          537 t
ORIGIN      
        1 tcgttgccat gacccgcgaa gagctaccgc ggttggagga attgcaccgc cggggagaag
       61 cgaacggttt gcgcgggctt caattaatcg atacgggacg cttaaacgag cttgagccac
      121 acgccaccgg aatcaaagga ctctatgtgc cggagaccgg catcatcgac tacaaaaagg
      181 tggccgccgc atacgctggg aagattcgtg attcgggcgg cgacatccgg ctgtcgcaaa
      241 aagttgtcgg catactcgac cgtcccgagg agatcgtgct gcagacttcc ggtggagatt
      301 atcgcacgaa gcatctgatc aattgctgcg gcctgcaatc ggacttgatc gcacagatga
      361 cccgcagcgc cgatcaagac ggcgggctgg agcatcgcat cattcccttt cgtggcgagt
      421 attataaaat cacgcccgaa cgggagttct tggtaaaaaa tctaatttat ccggttcccg
      481 atccgacttt tccgttcttg ggcgttcact tcacgcgcat ggccaaaggt ggcgtagagg
      541 caggacccaa cgcggtgctc gcttacgcac gggaaggata cagccacagc cagttcaacc
      601 tcaacgacct ctggcgaacg atttctttca aaggattttg ggcaatgacc ggaaagtatt
      661 ggcagactgg cttcggcgag ctttatcgtt cacttaacaa agccgcgttc gtgcgcgcct
      721 tacaaaaact ggtgccggat attcgcgaaa acgatctcgt accgggaggc gccggcgtgc
      781 gcgcccaagc ggtctcagcc aacggcgctt tggtggatga ctttgtaatt aagcaaggcc
      841 gtaatgctat tcacgtactg aacgctccgt cgccgggtgc gacggcttcg cttgccatcg
      901 gccggcagat ctgtgagatg gcggaaaaga atttccgcct gagaaactag ctctaagccg
      961 gttgagacgt catctcgcgc atcccttcga cacccgatca aggcatcttc tcaccccgct
     1021 gctaaacggt cgcggaataa aggtgaggat ttgccgcaaa acgatcacag ccgggaaatg
     1081 tcggcgtgcg gtccaggtga aatccgtcgg agtaatttct cggcggatag aaagagcgcc
     1141 cgtcataaga gctggaactg attttgccgg cttggccgac cgtgtaacta tgaagagctt
     1201 tgcattcgct gccaaccttg atttccactt cctcgtcaaa tatcgtggcc gaatattctg
     1261 cggaggaaac catctgaatg atcatcgaat ataagggcaa gaaaccggct gtctctcccc
     1321 aagccttcgt tgcgcccacc gctgtgctca tcggtgatgt tagcgtcggc gccgacgcca
     1381 gcatctggtg gggagcggtc ctgcgctcgg atcagggcgg ctttccaatc gtgatcggcg
     1441 cgcgcaccag cattcaggat aactgcgtga tccattcagg cgaagcttcg acgatcatcg
     1501 aagaagactg cactgtgggt catggcgcga ttatggaagg ctgtgtgatc aagcgcggcg
     1561 ccataatcgg catgaattcc acaatcctgg agaataccga gataggcgaa gagtcgctca
     1621 tcgccgccgg cagtgttgtt gttccacata ctaagatccc gccgcatgtt cttgccgccg
     1681 gcacgcccgc gaaagtgaaa aaggcgatag aaggcgaagc tctcaaatgg atcaagatgg
     1741 gcgcatcgac ttacgtcgaa ctctgccgca gctatttagg cggcgactac aaaatcgtcg
     1801 gctagttcac gaagatcgtc gaataggagg aatagagcgt gcgcatcatt ttgattggac
     1861 aagcagcgtt tgcagagaag acgctcgata aactcgttaa aaagggggaa gaggtgctgg
     1921 ccgtgttttg ttttcccgat gcgcctggtg gaaaaatcga tccggtcaag cagagggctc
     1981 ttcagctggg tatcccggtg catcaacata agacactcaa aacaccggaa gtcacagaac
     2041 agtttgtttc tctcaaagca gacttggcga tcttggcatt tgtcagccag atcattcccc
     2101 cgccggtatt caatgcgccg cgcttcggca gtatttgtta tcatccttca ctattgccca
     2161 agtaccgagg gcgcaccgcg atcaattggg ccctgatcag gggagaaacc gagaccggtt
     2221 tgaccatctt ttggttggac aagggaatcg atacaggccc catccttttg caaaaagaag
     2281 tgcgggttga cccggacgat accaccggca cgttgtattt caacaaacta tttccgctgg
     2341 gaatcgaagc gatcggcgaa gcggtggact tgattaaagc aggtaatccg ccgcggattg
     2401 tgcaagatga gtccaaggcg acttacgatc cgccgtgcgg cgatgaacac gccaagatcg
     2461 attggtccaa accggcccag gaagtttata attt
//
LOCUS       DASUTG010000016         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_742866, whole genome shotgun sequence.
ACCESSION   DASUTG010000016 DASUTG010000000
VERSION     DASUTG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742866"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1416)
                     /gene="glf"
                     /locus_tag="VF089_00345"
     CDS             complement(247..1416)
                     /gene="glf"
                     /locus_tag="VF089_00345"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX6172430.1"
                     /translation="MKLDVLVVGAGFSGSVVAERLAGRGLEVLLIDKRPHIGGNAYDT
                     LDRHGVLMHPYGPHIFHTNAPRVAEYLSQFTDWRPYEHHVLAKVGEQFVPIPINIDTV
                     NRVYGLKLTEETIQAFYDRVRQPRAQIRTSEDVVINAVGQDLYEKFFRGYTRKQWGLD
                     PSELSASVAARIPTRTNRDDRYFTDTFQKMPLDGYTKMFRRMLDHRRIHVEVGVDFFG
                     IQKKVRASHIVFTGPLDAYFGYCYGKLPYRSIRFEHEHLANTARYQPVGTVNFPNDYD
                     FTRITEFKHLTGQQHTGTSIVREYPQSDGDPYYPIPRPENELLLKKYQALAAKESKVT
                     FVGRLGQYRYYNMDQCVGAALKTADEIANVMVPQAALKTAVKPAVAATQQARAGS"
     gene            complement(1529..>2425)
                     /locus_tag="VF089_00350"
     CDS             complement(1529..>2425)
                     /locus_tag="VF089_00350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6172431.1"
                     /translation="LSENPLLSARFESPIQWFYSPMAAPYFLDDFDAAATVYDCMDEL
                     AQFRFAPPCLKEREQMLISRADVVFTGGYQLFQSKSRYHSNVHFYGCGVDAEHFSRAR
                     VPETEVPAEVGRLPRPVFGYFGVIDERLDYALLAQIAQEMPDASIVMVGPLAKVEQQS
                     LPHLSNIHWLGQRAYTDLPALVKSFDVCLMPFALNDATQYINPTKTLEYMAAGKPVVS
                     TAVPDVLHHFTPIVDVAHTAEEFIAAIQRSARSPRAELIGQGIDRANRASWDSIVSAM
                     RSHMLDSVRADSNAPSWQSAFP"
BASE COUNT          574 a          660 c          669 g          522 t
ORIGIN      
        1 cggaatcatc atccgaccca aatcagactg gagtgagaac gttgagatgc attcagattc
       61 tgaaaccgat accctgcgcc gcgaagccgg aacaacctcg gttcgacacg aaggtttaag
      121 tgcgagcgat cagcacaaat aatctgggag cgtccaaccg ttaagcttgc cagttgagca
      181 atcagttgcc gaggctctca aggtaaagca atgccgccgg cgaaacacga agagttgacg
      241 ttctcatcag ctacctgccc tggcttgttg cgtcgctgcc accgctggtt tcacagcagt
      301 cttcagagcc gcttgaggca ccatcacatt ggcaatctca tcggcggttt ttagcgccgc
      361 accgacgcat tgatccatat tgtagtaacg atactggcca aggcggccga caaaggtcac
      421 tttggattct ttagccgcga gggcctgata tttcttgagc agcaactcgt tctccggacg
      481 aggaatcgga tagtacgggt cgccgtcaga ctgcgggtac tcccggacaa tcgacgtccc
      541 ggtatgctgt tgtccggtga gatgtttgaa ttctgtgatt cgagtgaaat cgtaatcgtt
      601 gggaaagttg accgtgccca ccggctggta gcgcgccgtg tttgccaggt gttcgtgctc
      661 gaagcgaatc gagcgatacg gcagcttgcc ataacagtac ccgaagtacg catccagagg
      721 accggtgaag acgatgtggc ttgcgcgaac ttttttttga atcccgaaga aatcgacccc
      781 cacctccaca tggatcctgc ggtgatcaag catccgtcga aacatcttgg tataaccatc
      841 cagcggcatc ttttgaaagg tgtcggtaaa ataacgatcg tcacgattgg tgcgggtagg
      901 aatccgcgcc gccaccgagg cggaaagctc cgaaggatcg agaccccatt gtttgcgcgt
      961 gtagccgcga aagaattttt cgtaaagatc ctgcccgaca gcgttgatga caacatcctc
     1021 gctggtcctg atctgcgcgc gcggttggcg cacgcgatca taaaacgcct gaatggtttc
     1081 ttcggtaagc ttcagaccat aaactcgatt aacggtatcg atgttgatcg gaatcggcac
     1141 aaactgttcg ccgactttgg cgagcacatg gtgctcataa ggtctccagt cggtaaactg
     1201 cgagagatat tccgctactc gcggagcatt cgtgtgaaaa atgtggggtc cgtacggatg
     1261 catcaagacg ccgtgacggt cgagggtatc gtaggcattg ccgccgatgt gtggccgctt
     1321 atcgatgagc agcacctcca agccgcgacc ggccagcctt tccgcgacca ccgatccgga
     1381 aaagccggcg ccgacaacta agacgtccaa tttcattgaa ttcttccttt catatcattt
     1441 gagctcccga tcctcgggca aacccctgcg caattgaggg gagcctcaac atgcgggaaa
     1501 gcgaaccact agaaattatt gatcggcgtt atggaaacgc gctctgccac gacggagcgt
     1561 tcgaatcggc tcgcacgctg tcgagcatgt gactgcgcat ggcgcttacg atgctgtccc
     1621 acgaagcacg attggcgcga tctattccct gcccgattaa ctcggcacgt gggctccgag
     1681 cggagcgctg aatcgctgca atgaattcct ccgcggtgtg agcgacatcg acaatcggcg
     1741 tgaagtgatg caacacgtcg ggcacagctg ttgagacgac gggtttgccg gctgccatgt
     1801 attcgagtgt cttggtcgga ttgatgtact gggtcgcatc attgagcgcg aacggcatca
     1861 ggcatacgtc gaaagattta accagtgccg gcaggtccgt gtaagcgcgc tgcccgagcc
     1921 agtggatatt ggaaagatgg ggcaacgatt gctgttcgac cttcgccaat ggaccgacca
     1981 taacgatgga ggcatccggc atttcctgtg cgatctgcgc cagcagagca taatcgagcc
     2041 gctcatcgat gaccccgaag taaccaaaaa ccggccgcgg caatcttcca acttcggcgg
     2101 gcacctctgt ttccggcacg cgggctctac tgaaatgttc cgcatcgact ccgcacccgt
     2161 aaaaatgcac attgctgtga taacgggact tggattgaaa gagttgatag ccgccggtaa
     2221 acaccacgtc agctctagag atcaacatct gctcgcgctc tttcagacat ggcggcgcaa
     2281 accggaactg ggcaagctca tccatgcagt cataaacagt agccgccgca tcgaaatcgt
     2341 ccaggaaata cggcgcggcc atcggtgaat aaaaccactg aatcggagat tcaaaccgcg
     2401 cactgagcag tggattttcg ctgag
//
LOCUS       DASUTG010000017         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1928975, whole genome shotgun sequence.
ACCESSION   DASUTG010000017 DASUTG010000000
VERSION     DASUTG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928975"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..756
                     /locus_tag="VF089_00355"
     CDS             175..756
                     /locus_tag="VF089_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX6172432.1"
                     /translation="MISTRCHYFRELRPFLLTTLLLFALGITAGSLLAGHSTFAGLKI
                     NESLGGFAQMFSNLPKPLLALMIFTNNAVKTLLVIVLGTALAIVPLVFIVVNGVAIGV
                     VLHLATQSKGLAYSMMAIVPHGIFELPGVLCGAAIGMMLGGKAIKRLFRKSEFKMGSE
                     LSCALKIFATTIVPLLVIGAITEAYLTAAILGK"
     gene            complement(942..1736)
                     /locus_tag="VF089_00360"
     CDS             complement(942..1736)
                     /locus_tag="VF089_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX6172433.1"
                     /translation="MFLKEDSRGPAVVRLQKQLKKFGFNPGKIDGDFGPATKAAVIAF
                     QKSRGLLADGIAGPRTLAALKLVKSDALPSALPAATVTVVSKIFPFTPVDNIRLNLPH
                     VLNALRSERLGDKPLLLMALATIRAEAECFEPICEEKSKYNTSPGGHPYDLYDFRTDL
                     GNSQRGDGDRYKGRGFIQLTGRRNYEVHGRAIGLGDRLAQTPDLANDRDVAGKLLASF
                     LKAKERAIKEALLDNDLRLARRLVNGGVHGLERFCAAFTIGSRLIV"
     gene            complement(1799..2221)
                     /locus_tag="VF089_00365"
     CDS             complement(1799..2221)
                     /locus_tag="VF089_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6172434.1"
                     /translation="MSPFEIHPQLLHDCHRLGQLRLCHILLHKNAAVPWLILVPETVS
                     TGDLLDLPEHVRNVALDEAAIGARFIKNEFAVSKINFAAIGNVVSQLHLHVVGRRAND
                     PCWPAPVWGNLVEAHGYSPERIVTLTARLTQNHSLKAF"
     gene            complement(2232..>2492)
                     /locus_tag="VF089_00370"
     CDS             complement(2232..>2492)
                     /locus_tag="VF089_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6172435.1"
                     /translation="TLILTGIAGNICVLFTASDGYMRDFNLVVPQDCIASETEADNQH
                     ALAYMSKVLKADTRLSTDIDFKAMARQELDESRPQGTPAETM"
BASE COUNT          556 a          678 c          624 g          634 t
ORIGIN      
        1 accgtccaaa cgatcgacca gacggtccta ttactcttcg ttcgtacaaa taacgctgcc
       61 tccacctggg aatcaaccat caaatcaaag gtgagccgcg aattaaactt taggataaca
      121 gccctgtctg gtatcatagc gcgagtcttt tctagttccg ggaggaggtg atctttgatc
      181 tcgactcggt gccactactt tcgagagctg aggccgtttc tactcacgac cctgctgctc
      241 tttgccctgg gaatcactgc ggggagcctg ctggctggtc actcgacctt tgccggcctt
      301 aaaatcaacg aatcactcgg cggcttcgcg caaatgttta gcaatcttcc taaaccgctc
      361 cttgcgttga tgattttcac taacaatgct gtaaaaaccc tcctggtgat tgtgctcggg
      421 accgcgttgg cgattgttcc gctcgttttt atcgttgtta acggcgttgc catcggtgtt
      481 gtcctgcatc tagccacgca atcgaagggg ctcgcctatt ccatgatggc cattgttccc
      541 catggtatct tcgaactccc cggagtcctt tgcggcgctg ccatcggtat gatgttgggg
      601 ggtaaagcga tcaagcgcct ctttcggaaa tctgaattca agatgggttc tgaactaagc
      661 tgcgcgttga agatttttgc aaccactatt gtcccgcttc ttgtgattgg agcgatcacg
      721 gaagcgtatt tgacagcagc cattctcggt aaatagcact agcaggttgc tgaaaaagac
      781 ccatatgctt cgttgccgct caatcgccgc gctccaacgt actgtaagta cgcctccgct
      841 cgtcgatttt tcgcgcgcct cgcatctgga tctttttgag cagcctgcaa gaggtttttt
      901 cagcaccctg ctagagcttt taagcgttgt cacgtactgc atcaaacaat gaggcgactg
      961 ccgatagtga aagcggcgca gaagcgctcc aggccgtgaa ctccgccgtt caccagcctg
     1021 cgtgccaatc ttagatcatt gtcaagcaga gcctctttga ttgcccgctc cttagctttc
     1081 aagaaactgg ccagtagttt ccctgctaca tcacgatcat tggccaaatc cggcgtttgc
     1141 gccaggcgat cgcccaagcc gatagctctg ccatgcactt cgtagttacg tcgaccggtc
     1201 agctgaatga agccgcgacc cttgtagcga tccccgtctc cgcgctggct attcccaaga
     1261 tcggttcgaa aatcatacag atcatacgga tgaccgccgg gagatgtgtt gtattttgat
     1321 ttttcctcac agatgggctc aaaacattcc gcttccgcgc gaatggtcgc caaagccatc
     1381 agaagcaacg gcttatctcc caatctttcg ctgcgcaatg catttagaac atggggtaga
     1441 ttcaaacgga tgttgtcgac cggagtaaaa ggaaagattt tcgataccac cgtcacagtc
     1501 gcggccggca gcgctgaggg tagcgcatca ctcttaacca atttcagggc tgctagcgtg
     1561 cgcggtccgg cgataccgtc ggccaacagg ccgcggctct tctgaaaagc gatcaccgcc
     1621 gctttggtcg cgggtccgaa gtcaccgtcg attttgccgg gattaaagcc gaatttcttc
     1681 agctgctttt gcagtctaac taccgccggc ccacgtgaat cttcttttag gaacatcgtg
     1741 tttcacctat acccggcgga actccatgat gtcgagatct gcttcctgca gtttcgcttt
     1801 aaaaggcctt gagcgaatga ttttgagtca accttgccgt aagagtgacg atacgctcag
     1861 gggaataacc atgcgcttct accaggttgc cccaaaccgg tgccggccaa caaggatcgt
     1921 tggcgcgcct gccgacgaca tgaagatgaa gctgcgacac gacgttgccg atagcggcaa
     1981 agttgatttt gctgacggca aattcgttct ttataaaacg cgcaccgatt gccgcttcgt
     2041 caagcgccac atttctgacg tgttctggta gatcgagcaa atcacctgtt gaaaccgtct
     2101 cgggaaccag gattaaccac ggcaccgctg cattcttgtg cagcagaata tgacagagcc
     2161 ggagttggcc cagtcgatgg caatcgtgaa gcaattgcgg atgaatttca aagggagaca
     2221 tgtgcgataa tctacatcgt ttcggccgga gtgccctggg gccgcgactc atcaagctct
     2281 tgtctggcca tcgctttgaa atcaatatcg gttgacagcc gtgtgtcggc ttttaatact
     2341 ttcgacatat acgcaagcgc gtgttgatta tccgcttcgg tttcagaggc tatacagtcc
     2401 tggggaacca ccagattaaa atcgcgcatg taaccatcgc tggcggtgaa cagcacgcaa
     2461 atgtttccgg cgatgccggt taatatcaat gt
//
LOCUS       DASUTG010000018         7353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1484312, whole genome shotgun sequence.
ACCESSION   DASUTG010000018 DASUTG010000000
VERSION     DASUTG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484312"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..982
                     /gene="hisG"
                     /locus_tag="VF089_00375"
     CDS             83..982
                     /gene="hisG"
                     /locus_tag="VF089_00375"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX6172436.1"
                     /translation="MKAGKELKLGIPKGSLEEMTIELFRKSGWKISTTSRSYFPSIDD
                     ETLRCSLARPQEMSRYVETGTLDVAITGRDWVMENGSDVQLVCEMIYSKTSFRPTRWV
                     LAVPQDSPIEKIEDLRGKRISSEMVNYTKRYFAEREIPVEVEFSWGATEAKAAEGLVD
                     AIVEVTETGSTIRAHGLRIIHELMESCPQLIANKEAWQDSWKKEKIEQIRLLLLGALS
                     AENKVGIKLNVAEADLEKVIQLIPSITAPTVATLHPSPSLKGIRWFSVESVLEEAKVR
                     DLIPLLIRNGAVGIIEYPLNKVI"
     gene            complement(1143..2504)
                     /locus_tag="VF089_00380"
     CDS             complement(1143..2504)
                     /locus_tag="VF089_00380"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX6172437.1"
                     /translation="MLAKIELTETILRDAHQSLLATRMRTEDMLPIAAKLDAIGYWSL
                     ETWGGATFDAALRYLKEDPWERLRNLRAAIPNTRFQMLLRGQNIVGYHNYPDDVVVAF
                     IRRAAAGGIDVFRIFDAMNDLRNMKTAIEAALQTGKLVEGTICYTISPVHSVDYFLQV
                     GEQLAELGVQIICLKDMAGMLAPYAAQELISKIKARIPLPLHLHSHCTAGLAPMTYML
                     AIEAGVDIVDTAISPLSHGTSQPATEAVVAALMGTPRDTGLDLGKLNEIAEYFYGVRK
                     KYAEFESAVNNQVKTDILTSQIPGGMLSNLVAQLRQQKAEDRLDAVLSEMPHVRKDLG
                     YPPLVTPTSQIVGSQAALNVITGKRYSVVATETRNYVKGLYGRPPGPISEEIKTKVLG
                     GKEPIDCRPADLLKPGLEAARKEAGSLATSEEDVLTYALFPDIAKDYFLARDSGPTPQ
                     PFA"
     gene            complement(2530..3081)
                     /locus_tag="VF089_00385"
     CDS             complement(2530..3081)
                     /locus_tag="VF089_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172438.1"
                     /translation="MAAEATKKRKGTALLAVMDENCSSCAGSPLCEAHCPVDDCINLV
                     YEELPQGGLKPYRVMVDNDKCIGCQMCYSDDLTKVQQHKETGEIFYEYGGRFYDSNRN
                     PVPPDAVPKRFQLQLIGTESEDRLDKKICPWDAIKMYEFDDGLEVSQFFYDITKLRKI
                     DGGYVIDDAEKDRLEEKQSELYE"
     gene            complement(3071..3895)
                     /locus_tag="VF089_00390"
     CDS             complement(3071..3895)
                     /locus_tag="VF089_00390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172439.1"
                     /translation="MLRGWLVGLAVLACVGLSLAAETTSPLVKEKQWEEARNYLAEGK
                     AAEAKAVFESLLRRYPQEPDLHLFLGITLLRLRDPQAAESSVRKAIALDPDHVEARTL
                     LAWIESDIHGDLDAAIAEYKKVVELKPDLPQAYNNLGVALKRKGELAAAADKFNEALE
                     RQPDFSAALNNRGWTFADQDRWSEARRDFEQALKINPQDDGALYGLSQALREVRDYAG
                     AQEVLGRLISRSPNFVYWLEWGRIGLIRYWWVLLTIALVFFFKGRFQKARVESHGS"
     gene            complement(3896..4081)
                     /locus_tag="VF089_00395"
     CDS             complement(3896..4081)
                     /locus_tag="VF089_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172440.1"
                     /translation="MDEASGLQNFLAIVTKPDNIPIVGMLILVIFFSWVGLRQAFKND
                     KLVEQGKEDQIPNEMWK"
     gene            complement(4172..5761)
                     /locus_tag="VF089_00400"
     CDS             complement(4172..5761)
                     /locus_tag="VF089_00400"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6172441.1"
                     /translation="MENLETDILILHEGHEDEYGDDEDGIVMDEDHPMWRSERITLNS
                     VGIDIGSSTSHLIFSRLTLRRQGMALSSRFVVVNREMLHESPILLTPYVDKTTIDTNK
                     LNEFIHDSYRKANLTPSDIDTGAIIVTGEAAKKKNAEAIAALFSAEAGKFVCATAGHN
                     LESILAAYGSGAVGMTHHEGGDFTVMNVDIGGGTSKIAIAQGGKVIDTCAMEVGARLI
                     AMDENGRINRLEDTALKIANMAGLNLAIGATMSEEDKEKFAQVLCDSLFSVLERGPLS
                     SQAQDLLLTPNLEYKDSIHAVMFSGGVSEFVYGYEKRNLGDLGVHLGRRVRARANQLA
                     GGKIPLRPAEVRIRATVIGASQYTVQVSGNTIYLSHPELLPLRNLQVITPEFEQSESI
                     TSADITSAVQKALQRFDILDSDRAVALAIRWELGPSYPLIRMLAEGLVGAMKEHVDQG
                     QPMVLVFDADIAKLIGNIIERELIPGAGIISIDGIDLKDFDFIDIGQELPDAKAVPVV
                     IKSLIFRHSEQGRGHHHHHHH"
     gene            5902..>7353
                     /locus_tag="VF089_00405"
     CDS             5902..>7353
                     /locus_tag="VF089_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX6172442.1"
                     /translation="MSSSVIPMGQEEFQELSSFLKRFTRRFKFLKGIEGVCLTAICAL
                     LLLAIGPGVEQIKTFVPYAPLIYSVISVATLLGLILWTVVQLARRFSQEHAARYVEQK
                     QPKLRNNLINSLQLYPQVASAQAAPGFSAPMVLALLRTTRKEVSGLKLNDLLDTRRLK
                     SSLRLLVLLIIPVLGMVLFNPSWVGQTFSLLTHPLDHMPPSQTAIELKPQGVRVVRGA
                     PVTIEATASGAIPETLDLIVWAGANDRGELVGEEKLVMENLGEGKFRVKLAHLKRTLS
                     YRAVSGAFSSPTYFAEAIDPPEIGNVQLTVYPPAYTGLGSTSSTSGNIEGLKGSNLRI
                     NAISTKDIVKAEILLGDGKRVPLKIDGRKLQGNLVLFQSQQYQILVEDRHGFRNSPIS
                     YDLKVKPDGFPTIDLLHPTEDLEVNGDEILTLEYSARDDFGLAEVTLSVKVGERQEKI
                     RLQKDDARRLILRDQYRWDLGRLALREGEDALVN"
BASE COUNT         1667 a         2039 c         1926 g         1721 t
ORIGIN      
        1 gaacgggttt ttgatccgaa agaggtttac aagaaatcag tatgagcgcg cttcaaatat
       61 cggtcatcga ggtcgcagac tgatgaaagc cggcaaagaa ctgaagctgg gcattccgaa
      121 gggcagcctc gaggaaatga ccatcgagct gttcagaaaa tccggctgga aaatttcgac
      181 gactagccgc agttattttc cgtccatcga cgatgagact ttgcgctgca gtttggcgcg
      241 tccccaggaa atgtcgcgtt acgtcgagac cggtactctg gatgtcgcca tcaccggcag
      301 agactgggtc atggagaacg gttctgatgt gcagctcgtc tgcgagatga tctattccaa
      361 gacaagcttt cgcccgacgc gctgggtgct ggccgtgccg caggattcgc ccatcgaaaa
      421 gatagaagat cttcgcggca agcgaatttc ctcggaaatg gtgaactata cgaagcgcta
      481 tttcgccgaa agagaaattc ccgtcgaagt cgagttttcc tggggcgcca ctgaggccaa
      541 ggctgctgaa ggactagtcg atgccattgt tgaagtgacc gagaccggga gcacgatccg
      601 ggcccacggg ctaaggatta tccatgagct aatggaatcc tgcccccaac tgatcgccaa
      661 caaggaagca tggcaggact cttggaagaa ggagaaaatc gaacaaatcc gacttctgct
      721 gctgggcgcg ctttcggcgg aaaataaggt cggtatcaag ctaaatgtcg ccgaagccga
      781 tctggaaaag gtcattcagt tgattcccag catcacggcg cccacggtgg cgacacttca
      841 tccctctccc agcctaaaag gaatcagatg gttttcagtc gagagcgtgc ttgaagaagc
      901 caaggtcaga gacctaattc cattgctgat acgcaacggc gctgtcggta tcatcgaata
      961 cccgctcaat aaggttattt agcaggctgc ttgaaaaacc ctcatatgct ttgtttgcgc
     1021 taggtcgctt cactcgcgta ccgccgaggt acgcctcggt cttcgcgcgc ctagcctcta
     1081 aaggcatttt gagctgcgtg tacgcaaacg gctatgttgg taactgcttt ctggcgcaac
     1141 catcaggcga atggttgggg ggtcggtccg gaatcgcgcg cgagaaaata atccttcgca
     1201 atatccggga acaatgcgta ggtaagcaca tcctcttcgc tggttgcgag actaccggct
     1261 tccttgcgcg cagcttccaa acccggctta agaagatccg ccggtcggca gtcaatcggc
     1321 tcttttccgc cgagaacttt tgttttaatc tcctcgctga tcggtcctgg cggcctgccg
     1381 tacagtccct tgacgtagtt ccgagtttcg gtcgcgacaa cagaatagcg ttttccagtg
     1441 atcacattca gcgccgcctg ggaaccaacg atctgacttg tcggcgtcac taagggcggg
     1501 tagccaagat cttttctgac gtggggcatc tcacttagaa ctgcgtcgag cctatcttcg
     1561 gccttctgct gccgcaactg cgccaccagg ttggacaaca ttcctccggg aatttgtgaa
     1621 gtcaaaatgt cggtcttcac ctggttgttg acggcgcttt cgaattcagc gtatttcttg
     1681 cgcactccat agaagtattc ggcgatctcg ttcagtttgc caagatccag cccggtgtcc
     1741 cggggagtgc ccattaaagc cgccaccacc gcctccgtcg ccggctgtga tgtaccgtgc
     1801 gataacggcg agatggcggt gtcgactatg tcaacccccg cttcaatcgc tagcatgtaa
     1861 gtcatcggcg ctaacccggc cgtgcagtgg gagtgaagat gaagcggcag cggaatacga
     1921 gcttttattt tgctgatcag ctcttgcgcg gcgtacggag caagcattcc cgccatgtct
     1981 ttgaggcaaa taatctgtac gcccaattcc gccagttgct cgcctacttg caagaagtaa
     2041 tcgaccgagt gcaccgggct gattgtgtag cagatcgtgc cttcgacgag cttaccggtt
     2101 tgaagcgccg cttcgatggc ggtcttcatg ttgcgcaaat cattcatcgc gtcaaaaata
     2161 cggaagacgt cgatgccgcc ggccgcggcg cgccggataa atgcgacgac aacatcgtcc
     2221 ggatagttat ggtagccgac gatattttga ccgcgcagga gcatctgaaa acgagtgttg
     2281 gggatcgccg ctcgcaggtt gcgcaatcgt tcccaaggat cttctttgag ataccttaaa
     2341 gccgcgtcga aggtagcccc gccccaggtt tctaaagacc agtagccgat ggcgtcgagc
     2401 tttgcggcta tcggcaacat gtcctctgtg cgcatccgcg tggcgagcaa ggactgatga
     2461 gcatcgcgca aaatcgtttc cgtaagttcg atcttcgcta acataagttt aacctatcag
     2521 gcgcgtaagt tactcgtaca actcactttg cttttcttcg agacggtctt tttcagcgtc
     2581 atcgatcaca tagccgccgt cgattttcct gagcttggtg atgtcgtaaa agaactggga
     2641 aacttccaat ccatcatcga actcgtacat tttaatggcg tcccaggggc agattttctt
     2701 gtcgaggcgg tcttcggact ctgtaccaat aagttggagc tggaaacgct tgggaacagc
     2761 gtcaggcggc accgggttac gattgctgtc ataaaaacgg ccgccgtatt cgtaaaagat
     2821 ttcgccggtc tccttgtgct gctgcacctt cgtcaagtcg tcgctatagc acatttgaca
     2881 gccgatgcat ttatcgttgt ccaccatgac ccgataaggt ttgagcccgc cctgcggcag
     2941 ctcttcgtaa accaaattga tgcagtcatc gaccggacaa tgcgcctcgc acagaggcga
     3001 acccgcgcaa ctcgagcagt tctcatccat caccgctagc agtgccgtgc ctttgcgctt
     3061 tttggttgct tcagctgcca tgagattcga ccctcgcttt ctgaaaccgt cccttaaaga
     3121 agaataccaa tgcaatcgtc aataaaaccc accagtatcg aatcagcccg atgcgccccc
     3181 actccagcca gtaaacaaaa ttgggcgaac gagatatgag ccggcctaga acctcttggg
     3241 ccccggcata atcccttact tcgcgcaacg cctgcgacag cccgtaaagc gcgccgtcgt
     3301 cttgaggatt gattttaagc gcctgttcga agtcccggcg cgcttcgctc catctgtcct
     3361 ggtcggcgaa tgtccagccc cggttgttca gcgccgcgct aaaatccggc tgtcgctcca
     3421 atgcctcgtt aaacttgtcg gctgctgcgg caagctcgcc ttttcgcttc aaagccactc
     3481 caagattgtt gtaagcttgc ggaaggtcgg gtttcaactc cacgaccttc ttgtactctg
     3541 cgatcgctgc atcaagatcg ccgtgaatat cggattcgat ccaggcgagc agcgttctcg
     3601 cttcaacatg atcgggatct agagcaattg cctttcgcac cgatgattcg gcggcttgag
     3661 ggtcgcggag tctcagcaaa gttataccca aaaacagatg gagatcgggt tcctgcggat
     3721 atcgcctgag taaagattca aaaaccgctt tggcctctgc ggctttgcct tctgccagat
     3781 agttacgcgc ctcctcccac tgcttttcct taacgagagg agaagttgtt tcggcagcta
     3841 aagatagacc cacgcacgca aggacggcca acccaaccag ccagcccctc agcatctact
     3901 tccacatttc attgggaatt tgatcttctt taccctgctc gacgagctta tcgttcttaa
     3961 aggcctggcg cagtccgacc caactgaaaa agatcactag gatcagcatt cctacgatcg
     4021 gaatgttgtc cggcttggtt acgatagcga gaaaattctg taaaccgctt gcttcatcca
     4081 taaaagttaa gcacccgtaa aataaagata atttgaaagc tatgcgatat cattgccaat
     4141 gtcaacgtcg ccctccgcac tcgatcccgg ctcagtgatg atgatggtga tgatgaccgc
     4201 gtccctgttc gctatggcga aagatcaacg acttgatcac aacagggaca gctttggcat
     4261 cgggcaattc ttgaccgatg tcgatgaagt cgaaatcctt aagatcgatg ccgtcgattg
     4321 aaatgatgcc tgctcctggt attagctcgc gttcaatgat gttccctatc agtttggcga
     4381 tatccgcatc aaagaccaga accatcggct gtccctggtc aacgtgctcc ttcatggcgc
     4441 cgacaagccc ttccgccagc atgcgaatta gcggatagga cggacccagc tcccagcgga
     4501 tcgccagcgc tacggctcgg tcagagtcta ggatgtcgaa gcgctgtaaa gctttctgaa
     4561 cagcgctggt gatatcggct gatgtgatcg attcgctttg ctcaaactcc ggcgtaatca
     4621 cttgcaggtt gcgcaacggc agcaattccg gatgagaaag gtagatggta ttgccgctaa
     4681 cctggacggt gtactgcgac gctccgatga ccgtagcgcg aatccgaact tcggccggtc
     4741 gcagtggaat cttaccgccg gccagctgat tggcgcgcgc ccgaacgcgc cggcccagat
     4801 ggacaccgag gtcccccaga ttccgctttt cgtaaccata gacaaattcg gacactcctc
     4861 ctgaaaacat caccgcgtga attgaatctt tatattcaag gttcggcgtc aaaagcagat
     4921 cttgcgcctg agacgaaagc gggccgcgtt cgagcactga gaacagcgag tcacacagca
     4981 cctgcgcaaa cttctctttg tcctcttcgc tcatggtagc tccaatggca aggttcagac
     5041 ccgccatatt agctattttg agcgcagtgt cctcgagtcg attgatcctc ccgttttcgt
     5101 ccattgctat caggcgcgcg ccgacttcca tggcgcaagt gtcgatgacc tttcctccct
     5161 gcgcgattgc gatcttgctg gtgccgccgc cgatgtcgac attcatgacg gtgaaatcgc
     5221 cgccctcatg atgggtcatg ccgactgcgc cggaaccgta tgccgctaga atcgactcga
     5281 gattgtgccc cgcggtggca cagacgaact tccccgcttc cgcggaaaac agggcggcta
     5341 tggcctcagc gtttttcttt ttcgcagcct cgccggtaac gatgatcgct ccggtatcga
     5401 tatcgctggg cgtcagatta gctttgcggt aggaatcgtg gatgaactca ttcaacttgt
     5461 tggtatcgat agtcgttttg tcgacatacg gggtcagaag gatcggcgac tcatggagca
     5521 tctcgcgatt gaccacaacg aaacggcttg ataatgccat gccctgccga cgcaaggtca
     5581 ggcgggaaaa aatcaggtgc gatgtcgatg aaccgatgtc gatgccgacc gagttaagcg
     5641 taattcgctc ggagcgccac atcggatgat cttcgtccat gacgatgccg tcctcatcgt
     5701 caccgtattc atcctcgtgc ccctcgtgca gaatgagtat atcggtctca aggttttcca
     5761 ttaaactcgt gacgatcctt tctccctacc gtggcgcaaa agtgataaag ttttctttga
     5821 ttaacataag aaacatcgtc ttgccactca cgcggaactg agcccagagc acgatagaca
     5881 acccctagga tcggcgataa tatgagttcc agcgtcattc caatgggtca agaggaattt
     5941 caagaactct cctcattctt aaagcgcttt acccggcgct tcaagttcct caagggaatc
     6001 gagggcgtct gtctcacggc gatatgcgct ctgctgcttt tggcgatcgg accgggtgtc
     6061 gagcaaataa aaactttcgt tccctatgcg cccctgatct acagcgttat tagcgtcgcg
     6121 accctgttgg gcttgattct ctggaccgtg gttcaactcg cgcggcggtt ttcacaagag
     6181 catgcagctc gctacgtcga gcaaaaacaa ccaaaactgc gcaataactt aatcaactct
     6241 ttgcaactct acccgcaggt ggcgagcgct caggccgcgc cgggattttc cgcaccgatg
     6301 gtgctcgcgc tgctccgcac aacccgcaag gaagtttccg ggctcaagct caacgatttg
     6361 ctcgacacgc gccggcttaa gagcagtctg cggctacttg ttcttcttat cattccagta
     6421 ctcgggatgg tccttttcaa tccttcctgg gtcggccaga cgttcagctt gttgacgcat
     6481 ccgttggacc atatgccgcc ctctcaaaca gcgatagaac tcaagcctca aggagtgcgt
     6541 gtggtgcgcg gcgcgccggt gacgatcgag gcgaccgctt caggagctat cccggaaact
     6601 ctcgatctca tcgtctgggc cggcgcgaac gatcgcggcg agctcgtcgg tgaggaaaaa
     6661 ctggtgatgg agaatctggg cgaaggcaag ttcagggtca agttggctca tttgaaaaga
     6721 actttgagct atcgtgccgt tagcggcgca ttttcctcgc cgacttactt cgccgaagcc
     6781 atcgatccgc ccgagatcgg caacgtgcaa ttgactgtct acccgccggc gtataccggg
     6841 ctcggctcaa cgtcctcgac cagcggcaat atcgagggac tcaagggctc gaacctcagg
     6901 atcaatgcga tcagtacaaa ggacatcgtc aaagcggaaa tccttcttgg tgatggcaag
     6961 cgcgttccgc tcaagatcga tggacgcaag ctgcaaggca atttggtact ttttcagtca
     7021 cagcagtacc aaatattggt ggaagatcgt catggatttc gaaattcgcc gatatcctat
     7081 gatctgaagg ttaaacccga tggctttccc actatcgacc tgctccaccc caccgaagac
     7141 ttggaggtca atggcgacga aatcctaacc cttgaataca gcgcgcgtga cgatttcggc
     7201 cttgctgaag tgacgctttc ggtcaaagtc ggcgagcggc aggagaaaat ccgactgcag
     7261 aaagatgacg cccgcaggct aatcctgcgc gaccaatatc gctgggactt gggccggctg
     7321 gcattgcgcg agggagagga cgcgctggtt aat
//
LOCUS       DASUTG010000019         4158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2470, whole genome shotgun sequence.
ACCESSION   DASUTG010000019 DASUTG010000000
VERSION     DASUTG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VF089_00410"
     CDS             complement(<1..254)
                     /locus_tag="VF089_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172443.1"
                     /translation="MSCRVLVVTALILAGLVDTAAAQTSHGQPGDWDKIVEAAKKEGK
                     VVVSIPPSRKLRRAMEVAFTRRYGIDVEFVSARSSASIQK"
     gene            649..1572
                     /locus_tag="VF089_00415"
     CDS             649..1572
                     /locus_tag="VF089_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6172444.1"
                     /translation="MPLLLIALIAHALGCARQFPTTKVTPIKAANPAELQRYLLSRPD
                     VAQFRFRGPFAMVERRDLEIPLDSDLAVKADLYLCAAASKAPLVIVLHGNNNSKEDHA
                     FQAMHLASWGMHGLALDLPKRGPWIGNGRILAKLVDTIHRMSQLVDHRIDSDKIILVG
                     HSFGATAVATALGEGAPALGGVLLDPTAIGRGLSQSLTRIAVPVMVIGADEEIWPGRN
                     RGQFYRFIPRAIGEISIRDTVHEDAQYPNQHTLRTFEDEPDETEEAQIAFVSALTASA
                     FGLAATGNIDYAWNSFETAFKNGVFFNARRK"
     gene            1578..2480
                     /locus_tag="VF089_00420"
     CDS             1578..2480
                     /locus_tag="VF089_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172445.1"
                     /translation="MRTGNSSVPRWQTAGAGLAVLIIIIHVLGLKVGWSQEPVHPQIK
                     KEFLEQEKIYRSRAADGRKVYTTNRGLSRYAELLPSGFCDALGRLGSSDRWLDIGAGQ
                     GEAILDYYVPEEAAAERCRGSGPRARAVAISIEDRRTDDWKQQTASLGDDRIRYLFGK
                     RLRQYSVEELGTFQIISDVYGGFHYTEDLSQFVDKVLRLLEVGGGFYTLATGVHLADG
                     KEKLGSLYLTELEDAFGREVKLCSWLKQISCVQVTCESKSDRDRPTELIKIQKVCNDT
                     SVPRMKLVEFEAGYPPARCFQLDP"
     gene            2606..3424
                     /locus_tag="VF089_00425"
     CDS             2606..3424
                     /locus_tag="VF089_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172446.1"
                     /translation="MSSLLLAILYLLGSASFAAANTFDDALAVYERGDYVQAMKLFRQ
                     LAENGHQWAQRRVGLMYAEGQGVPQDYQEAVKWYGLAAAQGNVPAQYSLGLAYEKGKG
                     VPQDYQEAVKWYRIAATREDEWAQTRLGSIYAEGKGVPQDYQEAVKWYRLAAAQGYAP
                     AEYSLALAYEKGQGVPQDYREAVKWYRLAAAQGNEFAQVNLGVMYTHGRGVRQDLVRA
                     HMWFDLAAASSTGNPRDTATENRDRIASEMTAEQIATAQEMARRCQESKLKKCD"
     gene            complement(3686..3967)
                     /locus_tag="VF089_00430"
     CDS             complement(3686..3967)
                     /locus_tag="VF089_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6172447.1"
                     /translation="MKCPVCKEATLTMAERQGVEIDYCPQCRGVWLDRGELDKIIEKS
                     LADDVGGAPTGSRPVFRQPQPGGYSQQPRYDEGYYHRKKKWWHDMFDFD"
BASE COUNT          952 a         1155 c         1184 g          867 t
ORIGIN      
        1 attttttgaa tgctcgcgct gctgcgcgcc gacacgaact caacgtcgat gccatagcgg
       61 cgggtaaacg cgacctccat ggccctacga agcttacgac tcggcggaat agagacgacc
      121 accttccctt ccttcttcgc cgcttcgacg atcttgtccc aatcgccggg ctggccgtgg
      181 ctcgtttgag ccgcagcagt gtcaacgagg cctgccaaaa tcagcgccgt aaccactaag
      241 actctgcaac tcataccggc tctcttccga acacgagcaa tcattgaaaa agtttctcca
      301 ttaactaaat tccgggacag aacaatatac gagatggaca tctcattatg ttatgtcatc
      361 ttttcgttca agggctgaaa tacgaaaact ggattgccca atcgtggcgg ttgccggcac
      421 gtcggtccga gaatgattta ggagcgagtc tcgactgttg accaactcca aactcgttgt
      481 accttaccca tttataatga gcttgatatc aatggagccc gagtggtgaa aaactctgat
      541 cacggctggt caaaacgctc cagaagcaag gcgcgcttcc cgggaaattc gaatttcgaa
      601 tttcgaaatt cgaaattgat ccgtcggtgg tccgttttca acagcctgtt gcccctgctg
      661 ctgatcgcct tgatcgcgca tgcgctcggt tgtgctcgcc agtttccgac aactaaagtt
      721 acacccataa aggcagcaaa cccggccgag ctccagcgct atctgctgtc ccgtcccgac
      781 gtggcgcaat tcaggtttcg cggccccttc gccatggtcg agcgcaggga cctggaaatt
      841 cccctggatt ccgatctggc ggtgaaagcg gacttgtacc tgtgcgccgc ggccagcaag
      901 gcgccgctgg tcatcgtgtt gcacggtaac aacaattcca aggaggacca tgccttccag
      961 gcgatgcact tggcctcctg gggcatgcat ggcctcgctc tcgacttgcc caaacgaggc
     1021 ccgtggatcg gaaacggcag aatccttgcc aagctcgtcg acaccattca tcgaatgtcg
     1081 cagctcgtcg accaccgcat agactcggac aagatcattc tcgtcggcca ctcattcggc
     1141 gccaccgctg tcgccaccgc tttgggcgaa ggcgccccgg cgctgggcgg tgtcttgctc
     1201 gatcccaccg cgatcggtcg aggcttgtcg cagtcgctaa cgcggatcgc cgttccagtg
     1261 atggtgatcg gcgccgatga ggagatctgg cccggccgca accgcggcca attttaccgc
     1321 ttcattccgc gcgccatcgg agaaatttcg atacgggata cggtccacga agatgcacag
     1381 tatccgaacc agcacacgct gcgcacgttc gaggacgagc ccgatgagac cgaggaggcg
     1441 cagatcgctt tcgtcagcgc actcaccgcc tccgcctttg gccttgcggc caccggcaac
     1501 atcgactatg cctggaacag ctttgaaacc gctttcaaga atggggtttt tttcaacgcg
     1561 aggaggaagt agtcgtcatg cggacaggca attcgtcagt gccacgatgg cagaccgcgg
     1621 gagcgggcct tgctgttttg atcatcatca tacatgtgct tgggctgaaa gttggctggt
     1681 cgcaggaacc ggttcatccg cagatcaaga aggaattcct cgagcaggaa aaaatctatc
     1741 gcagccgggc cgccgatggt cgcaaagtct acaccactaa ccggggactc tcgcgctacg
     1801 cggagcttct tccctccggc ttctgcgacg ctctcggccg gttaggcagc tcggaccgat
     1861 ggctcgatat cggagcgggg caaggggagg ccatcctgga ttactacgta cccgaagaag
     1921 ccgcggctga aaggtgccgc ggctccggcc cgagggcgcg tgcggtcgcg atctccatcg
     1981 aagaccgccg gaccgacgac tggaaacagc agaccgctag cctcggggat gaccgcatac
     2041 gttatctctt cggcaagcgc ctgcggcaat attcagtcga ggagcttgga acattccaga
     2101 taatttccga cgtgtacggc ggcttccact atacagaaga cttgtctcag ttcgttgaca
     2161 aggtgctgag gttgctggag gtcggcggtg gcttctacac gctggcgaca ggcgtgcacc
     2221 ttgcggatgg caaggagaaa ctcggtagcc tttatcttac cgagctggag gatgccttcg
     2281 gccgcgaggt aaaactgtgc tcctggttga agcagatctc atgcgtgcaa gtcacctgcg
     2341 agtcgaagag cgatcgggat aggcctaccg agctgataaa aatccagaag gtttgcaacg
     2401 acacctcggt cccgcgcatg aaactagtgg agttcgaggc gggctatcct cccgcgcggt
     2461 gtttccaatt ggacccgtag tccgcgtgcg actaattccg ggctgatcaa aacggttcag
     2521 atgcgaggcg cggaaaagac acgtatgaaa gcggaaggat aaaacggcaa tttctgcttt
     2581 cagctttcat aattcatccc ttctgatgag cagcctgctg ctcgccatcc tttatttgct
     2641 gggtagcgct agctttgccg cagcaaacac ctttgatgac gctctcgctg tgtacgagcg
     2701 cggcgactac gttcaggcga tgaaactgtt tcgccagctt gccgagaacg gacaccagtg
     2761 ggcgcagcgg agagtaggtt tgatgtatgc agaagggcaa ggcgtgccgc aggattatca
     2821 ggaggcggta aagtggtatg gcctcgcggc ggcgcaggga aatgtcccgg cacagtacag
     2881 cctgggtctg gcgtatgaga aaggtaaagg cgtgccgcag gattatcagg aagccgtcaa
     2941 atggtatcgc atcgcggcga cgcgggaaga cgaatgggcg cagacaaggc tgggttcgat
     3001 ctacgcagag gggaaaggcg tgccgcagga ttatcaggaa gctgtaaagt ggtatcgcct
     3061 cgcggcggcg cagggatacg caccggcaga gtacagtctg gctctggcgt atgaaaaggg
     3121 tcaaggcgtg ccgcaggatt atcgggaagc cgtgaagtgg tatcgcctgg cggcggcgca
     3181 gggaaacgaa ttcgcgcagg tcaatctggg tgtgatgtat acccatggca gaggcgtgcg
     3241 gcaggacttg gtgcgtgcgc atatgtggtt tgatctcgct gccgcgtcat cgaccggcaa
     3301 tcctcgagat acggcgacag agaatcgaga tagaattgcg tcagaaatga cagccgagca
     3361 gattgcaacc gcgcaggaga tggcacgacg ctgtcaggag tcgaagctta aaaagtgcga
     3421 ctgaagcata atgcactccg atcaggtgaa gaaggagtca cccggatcat cagcgcgccg
     3481 tgagcccatg tgaaacttat gaaggtacgg ttgagatcat caaggaaaac aggataggcg
     3541 aaagcaaaga aacccggccg ttgtcgaggc gtagtttcag actcatgcct cactctaacc
     3601 tcccctcctc taccctgacc ccagaacgcc tactttgaat ctcccactga gggaggaaaa
     3661 gcatccaaga ctcgctccga ggagctcagt caaagtcaaa catatcgtgc caccacttct
     3721 ttttgcgatg ataatagcct tcgtcatacc gcggctgctg cgaatagccg ccgggttgag
     3781 gctgccggaa taccggccga gaacccgttg gagcaccacc aacatcatcg gcaagggact
     3841 tctcaatgat cttgtccaac tccccgcgat ccaaccagac gccgcggcac tgagggcaat
     3901 aatcgatctc aactccctga cgttcagcca ttgtcaaagt tgcttcctta cacacgggac
     3961 atttcatcta attctcctcg ataaaaattc actctcgcac tttcgccgtg agcgtaaaat
     4021 gctcgtgtgg cattttcgcc tcggccgcaa catgatgtac gctgctcctt ttttggataa
     4081 aagatttgga gcttaagtca accacgagag cggcaaaatc tctatacgat aataggcata
     4141 ggtcttttgt ggggcgaa
//
LOCUS       DASUTG010000020         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2077785, whole genome shotgun sequence.
ACCESSION   DASUTG010000020 DASUTG010000000
VERSION     DASUTG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077785"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF089_00435"
     CDS             <1..474
                     /locus_tag="VF089_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6172448.1"
                     /translation="KGIDDVLVRFALCCHPLPGEHIVGFITRGRGVTVHTVGCPTILE
                     SDPHRKIEVTWEENVQAPRPVKIEVSCVDQPGLLAGISAAITSAKANIARAQIRTFTG
                     QKAVNTFEVMIKNSEHLKQVLQSILKVKGVYKAVRARGRTGGTPERDQAAKAEAR"
     gene            complement(494..685)
                     /gene="rpmB"
                     /locus_tag="VF089_00440"
     CDS             complement(494..685)
                     /gene="rpmB"
                     /locus_tag="VF089_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6172449.1"
                     /translation="MARICSLCGKGRSVGNSVSHANNKTKRTWRPNLQRVKGNFNGQV
                     KRALVCTDCIHSGRVQKVA"
     gene            complement(678..1157)
                     /gene="rfaE2"
                     /locus_tag="VF089_00445"
     CDS             complement(678..1157)
                     /gene="rfaE2"
                     /locus_tag="VF089_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX6172450.1"
                     /translation="MSGKLKSLEEIKAIVVGARNNGKKVAFTNGCFDLLHRGHVHVLR
                     AARACADLLIVGINSDQSVKQIKGPARPVLPESDRCELLAAMEMVDYVILFNEPDPYS
                     LISAIRPDVLVKGGDWNTDKIIGADLVKEAGGEVMVVPYMKGFSTTEIIERIRNSDG"
     gene            1316..>2573
                     /locus_tag="VF089_00450"
     CDS             1316..>2573
                     /locus_tag="VF089_00450"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172451.1"
                     /translation="MEYQDLRDWIQIADEMGELKTLKGCDWNLEIGAITELVAHKEDG
                     PAVLFEDIKGYPKGYRVLSNSLSSRKRLALTLGLPPGETKMDFVKIWKDNYKSIKPVP
                     AKFVKQSALFDNVYKDGDIDMFKFPTPFWHDKDGGRYIGTGSVDITRDPDEGWVNYGT
                     YRVMIHDKNKLGFYISPGKHGRIQREKYAANGKPFKIAMSFGHDPLTFLAGSIEVPYG
                     VPEYEFIGGLRGEPFEMIEGEYSGLPIPANAEIVIEGDVHFDNPTVEGPFGEWTGYYA
                     SAERAEPWVEVKRLYHRNDPIILGSPPGRPPAELGWYRSYLRSALIWDEMEKAGVPDV
                     KGVWLTVSGGSRLMMCVSIKQRYPGHAKQAAVIASQCHAGAYLGRFVVVVDDDIDPSN
                     TDDMIWAMSTRCEPEQDIDILRRCWSG"
BASE COUNT          592 a          687 c          705 g          589 t
ORIGIN      
        1 aaagggattg acgacgtgtt ggttcggttt gctttgtgct gccatccgct accgggagaa
       61 cacatcgttg gctttatcac gcgcggcagg ggcgtcaccg ttcatacggt cggctgtccc
      121 accattcttg aaagcgatcc ccatcgcaag attgaggtca cctgggagga aaacgttcag
      181 gcgccgcgtc ctgtgaagat cgaagtgagc tgtgtggatc agccgggttt gctagccggg
      241 atcagcgccg cgatcaccag cgccaaggcc aatattgcgc gcgctcagat ccgaaccttc
      301 accggtcaaa aagcagtgaa cactttcgaa gtcatgatca agaactccga acatcttaaa
      361 caggtgctgc agagtattct gaaagtcaaa ggggtctata aagccgttcg cgcgcgcggt
      421 cgcaccggcg gtacgccgga aagagatcag gcagccaaag cagaagctcg ctaaggctct
      481 ttaggtttaa acgtcaggcg actttttgaa cccgacctga atggatgcaa tcagtgcaca
      541 ctagggcgcg tttaacttga ccgttgaagt tgcccttcac gcgctgcaag tttggacgcc
      601 atgtgcgctt ggttttgttg ttggcgtggc tgacgctgtt tccgaccgag cgacctttgc
      661 cgcataggga gcaaattcta gccatctgag ttccttatcc tctcgattat ttctgtcgtt
      721 gaaaaacctt tcatataggg aacgaccatt acctcgccgc cagcttcctt taccaaatcc
      781 gcaccgatga tcttatccgt gttccagtca ccgcctttga caagcacatc gggtcgtatg
      841 gccgagatca gactataagg atccggttcg ttgaatagaa taacataatc gaccatctcc
      901 atcgcggcta acaactcgca gcgatcggac tccggcagca ccggccgtgc aggtcccttg
      961 atctgcttga cggattgatc gctgttgatg ccgacgatca gcaaatctgc gcaagctctt
     1021 gctgccctta gaacatgtac atgtcctcgg tgaagcagat cgaagcaccc gttggtgaaa
     1081 gctactttct tgccgttgtt gcgtgctccg acgacaattg ccttgatttc ctcaaggctt
     1141 ttgagcttcc ccgacacagt caatgggtag catgatcagc ccgcgttttc aattataact
     1201 actcgtgatt ccaagcttat cgacttcttg accgctttgg taaatttggt taaggtctgg
     1261 ttaacaagcc gggcgtcttt gcagctcatc taccgaaaaa acttaggagg tatcaatgga
     1321 atatcaggat cttagagatt ggatccaaat tgccgacgaa atgggcgagc tcaagaccct
     1381 caaggggtgc gattggaatc ttgagatcgg tgccatcacc gagctcgtgg cccacaaaga
     1441 agacggcccg gcggtgcttt tcgaagacat caaaggttat cccaagggct accgcgtact
     1501 gtcaaattct ctctcgtcgc gcaagcggct ggcgctcacg ttagggcttc cgcctggcga
     1561 gaccaagatg gacttcgtca agatctggaa agacaattac aagagcatca agccggtgcc
     1621 ggcaaagttc gttaaacaga gcgcgctgtt cgataacgtc tacaaagacg gcgatatcga
     1681 catgtttaaa tttccgacgc cattttggca cgacaaagac ggcggccgtt atattggcac
     1741 cggcagcgtt gacatcacgc gcgatcccga cgagggatgg gtaaactacg gcacttaccg
     1801 ggtgatgatc catgacaaga acaagctggg attttatatt tcacccggca agcacggacg
     1861 cattcagcgg gaaaagtacg cggccaacgg caagcctttc aagattgcca tgtccttcgg
     1921 gcatgatcct ctaacttttc tagcaggcag catcgaagtg ccgtacggcg tgcctgaata
     1981 tgagtttatc ggcgggttgc gcggcgagcc cttcgaaatg atcgaaggtg agtattccgg
     2041 cctgccgatt ccggccaatg ccgagatcgt catcgagggc gacgttcact tcgacaatcc
     2101 gacggtcgaa gggccgttcg gcgagtggac cggctactac gccagcgctg aacgcgctga
     2161 accctgggtc gaggtaaagc ggctttatca ccgcaatgac ccgattattc tcggctcgcc
     2221 tccgggccgg ccgcccgcgg agttgggatg gtatcggtcc tatctccgat cggctcttat
     2281 ctgggatgaa atggaaaaag ccggcgtgcc ggatgtaaag ggagtatggc ttacagtatc
     2341 cggtggtagc cgattgatga tgtgcgtctc catcaaacag cgctatccgg gccatgccaa
     2401 gcaggcggcg gttatcgctt cgcagtgtca cgcaggagcg tatctcggac gctttgtcgt
     2461 cgtcgtcgat gacgacattg acccatcaaa taccgacgac atgatctggg cgatgtcgac
     2521 ccggtgcgaa cccgagcagg acatcgacat tctacggcgc tgctggagcg gcc
//
LOCUS       DASUTG010000021         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_298895, whole genome shotgun sequence.
ACCESSION   DASUTG010000021 DASUTG010000000
VERSION     DASUTG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298895"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VF089_00455"
     CDS             <1..829
                     /locus_tag="VF089_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX6172452.1"
                     /translation="SISQLVSTAWASAASFRGTDKRGGANGARIRLAPQKGWKVNNPP
                     EVSKALQALEKIQQDFNRSQSGGKKVSLADVIVLGGCAAVEQAAKNAGYNITVPFAPG
                     RTDASQEQTDVESFAALEPTFDGFRNYLDGGEELSPETLLVERAFMLNLTAPEMTVLV
                     GGMRALDANSGQSKHGVFTQRPGTLSNDFFVNLLDMGTEWKASGSDKNVYEGRDRATD
                     AVKWTGTAVDLVFGYNSQLRALAELYAQEDSKEKFARDFVAAWNKVMNNDRFDLAQS"
     gene            complement(1378..2148)
                     /locus_tag="VF089_00460"
     CDS             complement(1378..2148)
                     /locus_tag="VF089_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6172453.1"
                     /translation="MLIDSHAHLQGKEYSGDVEAVIARAREAGIDKIIAVGGAGDLSS
                     NHDAVALANVYDNVYATVGMHPHDAKDVGPEELRSLRDLTANPKVIAVGETGLDYYYS
                     HSPHDVQRRVFAELIQLARATGLPLVVHERDAARDAAQLLRSEGKGGLSGVLHCFTGN
                     YEAACAYLDLGFYLSFTGIITFKNAEPLRDVVRQVPLEKILIETDSPYLTPVPHRGKR
                     NEPAYVRLVAEAIAKIKGVSLQEVAQITSGNVGNLFKI"
     gene            complement(2190..>2873)
                     /locus_tag="VF089_00465"
     CDS             complement(2190..>2873)
                     /locus_tag="VF089_00465"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6172454.1"
                     /translation="SKSLGNTISPLEMMKPPIGMDAFRYFVLRESVFGQDADFRRESL
                     IGRYNADLANNLGNLVSRVLAMQGKYFDGVIQPLSADWAPEDIELRDRFVHAENELNG
                     YMRELQFHRALEAVWSALDAANRYVVQTAPFTMIKDAEKKARVGEVLHHLLEAVRTIA
                     RVLAPFMPDTGLKLRELLAIGEDRLNAPWGHGLTAGHKVNPPIVLFSRIIEPGTAELK
                     LGINSPKVL"
BASE COUNT          629 a          844 c          790 g          610 t
ORIGIN      
        1 ctccatctcc cagctggtgt ccaccgcctg ggcatccgcg gcgagctttc gcggcacaga
       61 caaacggggc ggcgccaacg gggcgcggat ccgcctcgcg ccacagaaag gttggaaagt
      121 gaacaacccc cccgaggtgt cgaaagcgct gcaagcactc gagaagatcc agcaggactt
      181 caaccgctca caatccggcg ggaagaaggt ctcgcttgcc gatgtgattg tccttggcgg
      241 gtgcgcggcg gtcgagcagg cggcgaagaa cgccgggtac aacatcacgg tcccgttcgc
      301 gccggggcgc acggacgctt cgcaggagca aaccgacgta gagtcgttcg ccgcgctcga
      361 accgaccttc gatgggttcc gcaactacct cgacggcgga gaagagctgt cgccggaaac
      421 gctgctggtg gagcgggcct tcatgctgaa tttgacggct cccgaaatga cggtactcgt
      481 cggcggcatg cgcgccctgg atgccaactc gggccaatcc aaacatggcg tcttcaccca
      541 gcggcctgga acgttgagca acgacttctt cgtcaacctg ctcgacatgg gcacggaatg
      601 gaaggcgtcc ggctcggaca agaacgtgta cgagggtcgc gatcgcgcca ccgacgcggt
      661 gaagtggacc ggcaccgcgg tcgacctcgt gttcggctac aactcgcagc tccgagccct
      721 ggccgagctc tatgcccaag aggactcgaa ggagaagttc gcgcgtgact tcgtcgccgc
      781 atggaacaag gtgatgaaca acgatcgctt cgacctcgcc caatcgtagc atgaacggcc
      841 ggcggacggc gttgcgcgtc gtccgccggc cgttaccctg caacaatttc tagtcccgaa
      901 ttctaaacca cccaagcgac gtggcaacct ccgcttgctg gccgccggat tttcaaacat
      961 ccggctactt ccgcagcacg gccgcttagt ccttcttgaa gatctgggac aggtagtttg
     1021 gcagtccaac tcttcgatct gatgcagctg cgtttcgatc cagtcgatgt gatgttcctc
     1081 gtcaacgacg agatgttcga ccagctcacg tgtggcgtcg tcttgagcat cgaggcagat
     1141 cttattcccg gcctaagaac cgtcggcgcc gcctgcttcc aatccgagat cgttctcgga
     1201 ttactgcatg acgttcgatc ccgagttgga tcttgtcata ggcatgaaca ttgcgcacgc
     1261 cagcgagaag atgacctcgg tattctctct cataactcgt cctcctcgct gcaacgtcag
     1321 ttaatcttgt acacagcaat cacaaagaag aagaccaaaa gagtgccgaa gttcgcgcta
     1381 aattttgaac aggtttccga cgtttcccga ggtgatttgt gcgacctctt gcagagaaac
     1441 gcctttaatt ttggctatag cctcggcgac caacctcacg tacgctggct cattgcgttt
     1501 gccgcgatgc ggcaccggag tgagataagg cgagtcggtt tcgataagta ttttttctaa
     1561 aggcacttgc cgcacgacgt cgcgcagcgg ctcggcattt ttaaacgtaa taattccggt
     1621 gaaagagaga taaaatccca aatccaagta ggcgcaggca gcctcataat ttccggtaaa
     1681 gcaatggaga acgcccgaca gaccgccctt tccttccgag cgtagaagtt gagcagcatc
     1741 cctagctgcg tcgcgttcat ggaccaccag aggcaatcca gtggcgcgtg cgagttgaat
     1801 aagctcggcg aacactcgcc gctgaacgtc atgcggcgag tgactgtagt aatagtcgag
     1861 acccgtttcc ccgaccgcga tcactttcgg attggcagtc aggtctcgta agcttctcaa
     1921 ttcttccggc ccgacatcct ttgcgtcatg aggatgcatg cctacggttg cgtaaacgtt
     1981 gtcatataca ttagctagcg ccacggcgtc atgattgctc gacaggtcgc cggcgccacc
     2041 gacagcgatg attttatcga ttcccgcctc acgcgcgcgc gcgatgaccg cttcaacgtc
     2101 gcctgagtac tccttccctt gaagatgggc gtgactgtcg atcaacatat ttacgggagt
     2161 aaccggctct tgcgatcggt ctcagtgcgt cacaagactt taggtgaatt aatgcccagc
     2221 ttcagctcag cagtcccggg ttcgattatg cgcgagaata gaactattgg cggattgact
     2281 ttgtgaccgg cggtcaagcc gtggccccac ggtgcattca atcgatcctc accgatcgcc
     2341 agtagctcgc gtagcttgag cccagtgtcc ggcatgaacg gcgcgagtac acgggcgatc
     2401 gtgcgcaccg cttcgagcaa atggtgcagc acttcaccaa ctcgggcttt cttttccgcg
     2461 tccttaatca tggtgaacgg agcggtctgt acgacatagc gattagcggc atcgagagcg
     2521 gaccagaccg cttcaagagc cctatgaaac tgcagctctc tcatgtaacc gttcagttca
     2581 ttctccgcat ggacgaatct gtcacggagc tcgatatctt ctggcgccca gtcggcgctc
     2641 agcggttgaa tcacgccgtc aaaatatttc ccctgcatag cgagaacgcg actgactaag
     2701 ttgccgaggt tattggccaa atcagcatta taacgtccga tcaagctctc tcgccgaaaa
     2761 tcggcatcct gtccgaagac gctctcgcga aggacaaaat aacgaaaagc gtccatgcca
     2821 atcggtggct tcatcatctc taacggggaa atggtgttgc ctagactctt cga
//
LOCUS       DASUTG010000022         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_299032, whole genome shotgun sequence.
ACCESSION   DASUTG010000022 DASUTG010000000
VERSION     DASUTG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299032"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF089_00470"
     CDS             <1..323
                     /locus_tag="VF089_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX6172455.1"
                     /translation="ASFSDPVILIAGGVDKGGDYGPLQKCIQQRVRRLVLFGAAKHVI
                     AEALGHLTETVIVADLAAAVADASVHARPGDVVLLSPACSSFDMFRNYAERGQAFKSL
                     VCAL"
     gene            320..1414
                     /gene="ftsW"
                     /locus_tag="VF089_00475"
     CDS             320..1414
                     /gene="ftsW"
                     /locus_tag="VF089_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX6172456.1"
                     /translation="MRDALAVDKWIFFSVLALMAVGLTMVLSTSYLYAQERFGDGIYF
                     FRKQLIAMGAGLVCLILGSLMPAALLRRLAYPLLAVTLIVLILVLIPGIGAMRGGARR
                     WLMLPGFAFQPGELAKLALVFYLAHSMARKQEKIKTFAVGVLPHLMVASAILGLLLLQ
                     PDFGTALILTVVLYLMLFVGGTRVPHLLGTGALALPVLAYVMLTAEYRLRRLLTFMDP
                     WRDASNSGFQVIQSLIAFGSGQLWGRGLGESRQKLFYLPEAHTDFVYSVIGEEMGLLG
                     ALAVLALFGIILMRGLRLTSKIEEPFEQYFAFGLTVLLGMQGLIHMGVVMGLMPTKGL
                     VLPFISYGGSAIVVNLAEVGLLLGLSRRRL"
     gene            1415..2482
                     /gene="murG"
                     /locus_tag="VF089_00480"
     CDS             1415..2482
                     /gene="murG"
                     /locus_tag="VF089_00480"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX6172457.1"
                     /translation="MRVILAGGGTGGHLFPGLAVAREFQRRDAMTEILFVGTEKGIEA
                     RVLPREGFRLETITVKGLKGRGVRGLLDALYGIPAGLLQSLRIVKGFRPDCVLGLGGY
                     ASGPLLLAAKLRGIRCAIMEQNLQPGFTNKMLARFVDKIFTSYRETASYLPRKKVLET
                     GNPVRWQRLPDAPKGERFTLLIFGGSAGAHRINMAVLDALKQMTDLASQLRIVHQTGA
                     ADFPAISKAYQALPFDAEALPFIDKMDDAYARADLVLCRAGATTVAELTAFGKAALLI
                     PYPYAIYDHQRWNAQALADTGAAEMILEQELNGAALGLRLRAYVADPKKIESMARAAL
                     AMGRPEAAARIVDECYELAGA"
     gene            2506..3876
                     /gene="murC"
                     /locus_tag="VF089_00485"
     CDS             2506..3876
                     /gene="murC"
                     /locus_tag="VF089_00485"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX6172458.1"
                     /translation="MLHKKHRIHFVGIGGIGMSGIAEVLLNSGYAVSGSDLHDSEATR
                     RLRNLGAQIFVGHQEENLTVNPSVVVISTAVKYSNPEVLEARRRQIPVIPRAEMLAEL
                     MRMKYGIAVAGSHGKTTTTSMTAAVLSLAGLDPTMVIGGRVHMLGTNAKMGQGEILVA
                     EADESDGSFLLLTPTIAVVTNIDREHMDFHQTMERLTESFLTFVNKIPFYGLAVLCID
                     NPNVRAVLPKVRKRFATYGLSPDADFSVQDLHMKAGGVEFTVVHHGKSLGKLKLQLPG
                     RYSATNALAAVTVAHELEIPFGRTAEALAAFNGIHRRFEIKGQIRDIVVIDDYGHHPV
                     EVRATIGAIRESWKRPLTVIFQPHRYSRTQDLFDDFLTAFEGADRLVLTEIYGAGEDP
                     IPGVTGEALYHAIKRQGHMDVEFIADKTSIAAHVAGRLAAGDVVLTLGAGDIYKVGES
                     IVAALA"
BASE COUNT          775 a         1035 c         1181 g          935 t
ORIGIN      
        1 tggcgagttt ttccgacccg gtgattctca ttgccggcgg cgttgataaa ggcggcgatt
       61 acgggccact gcaaaagtgc attcagcagc gagtgcgacg cttggtgttg tttggagcag
      121 ccaaacacgt catcgccgaa gcactcggtc acctcactga aacggtgatc gtagcagatc
      181 tggccgcggc agtggccgac gcctccgtgc atgcgcgacc cggcgacgtc gtattgttgt
      241 cccccgcgtg ttccagcttc gacatgtttc gcaactatgc cgaaaggggc caagccttca
      301 aaagcctggt ttgcgcctta tgagagatgc gctggcagtt gataaatgga ttttcttctc
      361 tgttctcgca ctgatggcgg tggggctcac catggtcctc agcaccagtt atctttacgc
      421 tcaggaacgg tttggcgatg ggatctattt ctttcgcaag cagttgatcg ccatgggcgc
      481 gggcttggtt tgtttgatac tcggatccct tatgccggcg gcgctgttgc gccgtctcgc
      541 ctatcccttg ctggcagtga ctcttattgt tttgattctg gttttgattc ctgggatcgg
      601 cgctatgcgc ggcggtgccc gacgttggct gatgctccct ggtttcgcat ttcagccggg
      661 ggagctggca aagctcgctc tggtttttta tctggcccat tcgatggcgc gcaagcaaga
      721 aaagatcaaa accttcgccg ttggtgtttt gcctcatctg atggtcgcca gcgctattct
      781 cgggcttctt ttgttacagc cggatttcgg cacggctttg attctgacag tcgtgctcta
      841 tctgatgttg ttcgtgggcg gcacgcgcgt cccgcatctc ttaggcaccg gcgcgttagc
      901 gcttccggta ctagcgtacg tgatgttgac agcggaatac cgtttgcgcc gcttattgac
      961 ttttatggat ccatggcgcg acgcatccaa cagcggtttc caagttattc aatcgctcat
     1021 cgccttcggt tccggtcagc tctggggccg gggactcgga gaaagccggc aaaagctttt
     1081 ttatttaccg gaggcgcata ccgattttgt gtactcagtt attggtgagg agatgggcct
     1141 gcttggcgcc ctggctgtct tggctctctt cggcatcatt ctcatgcgcg ggctgcgctt
     1201 gacgtcgaaa atcgaggaac cgttcgagca gtatttcgcc ttcggcctaa cagtcctttt
     1261 ggggatgcaa ggtctaattc acatgggcgt ggtcatgggg cttatgccga ccaagggcct
     1321 ggtcttgccg tttatcagtt atggcggatc ggccatcgtc gtcaatctgg cggaggtggg
     1381 actactgctg ggactctcgc gcaggagatt atagatgcgc gtgattttgg ccggcggggg
     1441 aaccggcggt catctgttcc cgggactggc cgtagcccgt gagtttcaac gacgtgatgc
     1501 aatgacggaa atcttgtttg tcggcaccga aaaggggatc gaagcgcgag tgctgccgcg
     1561 tgagggcttc cgtcttgaaa caatcaccgt caagggactt aaaggacggg gtgttcgcgg
     1621 cctgttggac gcactctatg ggattcccgc cgggctcctg caatcgctcc gaatcgtcaa
     1681 gggatttcgc cctgactgcg ttcttggcct cggcggatat gcatccggac cgctgttgct
     1741 ggcggcgaag ttgcggggca ttcgctgcgc tattatggaa caaaaccttc aaccgggttt
     1801 taccaataag atgctggcgc gctttgtcga taagattttt acttcatacc gcgaaaccgc
     1861 atcctacttg cctcgcaaaa aagtgctgga aacaggaaat ccggtccgtt ggcagaggct
     1921 gccggatgcg cccaaaggtg aaaggttcac cctgttgata ttcggcggaa gcgccggggc
     1981 gcaccgcatc aatatggctg tccttgatgc gctcaagcaa atgactgact tggcatcgca
     2041 gcttcgaatc gttcatcaga ccggcgcagc cgattttcct gcgatcagca aagcctatca
     2101 agctttaccg ttcgacgcgg aggcgctgcc ctttatcgac aaaatggacg atgcttatgc
     2161 gcgcgccgat ctcgtgctgt gccgcgccgg cgctacgacc gtggcggagc tcacggcgtt
     2221 cggaaaggcg gcgcttttaa tcccgtaccc ttacgcgatt tatgatcacc aacgctggaa
     2281 cgctcaagcg ttggccgata caggagccgc cgagatgatt ctcgagcagg aattgaatgg
     2341 cgcagcattg ggattacgtc tgcgggcata cgtcgcggat ccgaaaaaaa tcgaatctat
     2401 ggcacgagcg gctctcgcca tgggccggcc cgaggccgcg gcgcgcattg tcgatgagtg
     2461 ttacgaattg gctggggctt gaggcgaaag attgattaac agttcatgtt gcacaagaaa
     2521 catcggattc attttgttgg gattggcggc atcggcatga gcggtatcgc cgaggtgctc
     2581 ttgaactcag gatacgcggt gagcggttcg gatctgcacg acagcgaagc gacgcggcgg
     2641 ctgcgcaatc ttggcgcgca gattttcgtc ggccatcagg aagaaaacct gacagtgaat
     2701 ccctcggtgg tggtgatttc gacggcggtt aagtactcga acccggaagt gctggaagcg
     2761 cgccggcggc agatcccggt tattcccaga gcggaaatgc tcgccgagct gatgcgtatg
     2821 aagtacggca ttgcggtggc gggcagccac ggcaaaacca ccaccacttc gatgacggcc
     2881 gcggttctga gcctcgccgg gctcgatccg acgatggtca tcggcggccg cgttcatatg
     2941 ctcggcacta atgccaagat gggccaaggc gagatcctgg ttgccgaagc cgatgagagt
     3001 gacggcagtt ttttgttgct gacgccgacc atcgcagtgg tcaccaacat tgaccgcgag
     3061 catatggatt ttcaccagac catggaacgt ttgactgaaa gttttctgac atttgtcaac
     3121 aagatcccct tttatggact ggcggtgttg tgtatcgaca atcctaacgt acgcgccgtg
     3181 ttgcccaaag tgcgtaaacg gtttgccaca tacggtctgt ctccggatgc ggatttttcc
     3241 gttcaggatt tgcacatgaa agcgggcggc gtggaattca ccgtagtgca tcacggcaaa
     3301 tccctgggga agctgaagtt gcagcttccc ggccgctaca gcgcaaccaa tgcgctcgcc
     3361 gccgtgactg tcgctcatga gttggagatc ccctttggcc gcaccgcgga ggcgcttgcg
     3421 gcattcaacg gcatccatcg ccgattcgaa atcaaaggac aaattcggga tattgtagtc
     3481 atcgacgatt acggccatca tccggtggaa gtgcgcgcga ctatcggcgc aatccgggaa
     3541 agctggaagc ggcctttgac tgtgatcttc cagccgcacc gatacagccg cactcaggat
     3601 ctctttgacg attttctgac ggcgtttgaa ggcgccgacc gtttggtgct gaccgaaatc
     3661 tatggggcgg gagaggatcc catcccaggg gttacaggtg aagcgctcta ccacgcaatc
     3721 aagcgccagg ggcatatgga tgttgaattc atcgccgaca aaacgagcat tgcggcgcat
     3781 gtggccggtc gattggccgc cggggatgtg gttctgactc tgggagccgg agatatctat
     3841 aaagttggag aaagcatcgt tgcagctctg gcatgaacgg caactaacag ctcggggagc
     3901 agaattagtc ccaagctgtt taagcg
//
LOCUS       DASUTG010000023         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_151198, whole genome shotgun sequence.
ACCESSION   DASUTG010000023 DASUTG010000000
VERSION     DASUTG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151198"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF089_00490"
     CDS             complement(<1..839)
                     /locus_tag="VF089_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6172459.1"
                     /translation="MRVVDAISQILKREGVDFLACYPTNTLIEAAAAAGIKPVICRQE
                     RVGVGIADGYTRVLNGRRIGVFTMQAGPGAENAYSGITTAYSDSVPVLLLPVGHTRST
                     SQVHHFFRSTQSYATVTKWTEEISLASDTSDVMRRSFSLLKMGRPGPVLVEIPADVAI
                     EEIKEDELNYAPVRRTVSAANPQDIAEAARILVQAQAPIIIAGQGVLYAEASDELVEL
                     AELLQAPVTTPLEGKSAFPESHPLSLGTGAGVMPPTVHEFLRTADVVFAVGASLTRHS
                     IVAA"
     gene            complement(1125..1871)
                     /locus_tag="VF089_00495"
     CDS             complement(1125..1871)
                     /locus_tag="VF089_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX6172460.1"
                     /translation="MKALVLPPTPWLLMLVAVLIFWRRRWARKLLLLTFVLITALHSG
                     LVGYALRYPLESRYRPLLDPGALSYDAIVVLTGGMIPPEGLIPFSTIDEHMFRRLDEA
                     WRLYRIQPRPIILSGGHVNPFTPDKDENRIGREYLIRWGVPKHHVIGEGKSRDTFESA
                     IEVQKLLKRRGWKRYLLVTSALHMPRSMLAFKAKAPEPLAAPGDYSLGKLRLTPLDFF
                     PGEGAARGIYATLHEYIGIANYYWRIQFGS"
     gene            complement(2001..2645)
                     /gene="maiA"
                     /locus_tag="VF089_00500"
     CDS             complement(2001..2645)
                     /gene="maiA"
                     /locus_tag="VF089_00500"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX6172461.1"
                     /translation="MKLYTFFRSSASYRVRIALNLKGLEFEQAAIHLRRGGGEQLKPA
                     YKAINPQALVPALEDDGQAFSQSLAIIEYLEERYPKPALLPSDAADRAVVRSMALVIA
                     CEVHPIQNLRVLNHLKSDHKQSDEDTIRWARHWINLGFLALERMIVSVTKEGDFCFGQ
                     TPTMADLCLVPQLINARRFGVDLSAYPKLLAIEAACMSLPAFADAAPENQPDAE"
     gene            complement(2753..3724)
                     /locus_tag="VF089_00505"
     CDS             complement(2753..3724)
                     /locus_tag="VF089_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172462.1"
                     /translation="MTESNGAKKALHVATLSGWYRFEQDGREWKQTKRDLSYWSLTCM
                     SVDPDNPQKIYAGTEHSGLFYTADAGASWHRAQPNVPKMMLFAILALNGRVMVGTIPS
                     AVYRSKNGGWEELEGVRINSAGANFPPSPELQSRTRYLTFDPASPNRIYAGIEVGGML
                     VSDDDGQNWRPANEGLTDMDIHEVLASRNTAGLVFAACGEAAFRSFDRAAHWEEITPQ
                     SHDYGTSVAEDNDGIIYLGSAKGRPNGWIRKEGAMAAIFRSRDKGSTWEKIIDDLKGG
                     VMHMCPLPDGNGMIAGTSDGTLLVIDDSGAREIVTGLPFVTSVELAA"
     gene            complement(4045..>5185)
                     /locus_tag="VF089_00510"
     CDS             complement(4045..>5185)
                     /locus_tag="VF089_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX6172463.1"
                     /translation="ILFVAVLLYTATAFLKQTRAAFIIRGIFILAAIYILARYLDLQL
                     TAWLFQGFFAIFLIIIVVIFQEELRQIFERIAVWSLARKRTPTLGSSTSDVLVRTVAD
                     LAKEHVGALIVIRGNDPLERHITGGIPLEGKVSAPLLKSIFDPHSPGHDGAVMVDQDN
                     ISKFAAHLPLSKDLRQLANVGTRHSAALGLAELTDALCIVVSEERGTISVARDGKLRV
                     VQNIQELGAVLENFLQTKFPPATQNNFALPLFRENWVAKAIAIALAIGFWYIFVPGSK
                     TVEVSYTVPVSVENLPPDLQVEDIEPPVVNATFRGPRRAFYLFNSSKIKVAIDVSMAE
                     LGRRTFTISEKNIEYPKELTLQDISPSTLRLSVRKVPVANDTNRG"
BASE COUNT         1178 a         1427 c         1452 g         1128 t
ORIGIN      
        1 gcagcgacga tgctatgccg agtaagactc gcgccgaccg cgaagacgac atcggcggtc
       61 cgcaaaaact catgtacggt cggaggcatg acaccggcgc cggtcccgag cgacaatgga
      121 tgactttccg ggaatgcgct ctttccttcg agtggcgtgg tcacaggcgc ttgcagcaat
      181 tcggcaagct cgactaactc atccgacgcc tccgcgtaca agactccttg gccggcaatg
      241 ataatgggcg cctgcgcttg cacgagaatc cgagcggctt cagcgatgtc ctgaggattc
      301 gctgcgctca cggtacgtct gaccggcgca tagttcagct cgtcttcctt aatctcttca
      361 atggccacat ctgccggaat ctccacaagc acgggtcccg gccggcccat tttcaaaagg
      421 ctgaaggagc gtcgcatgac gtcgctggta tcggaagcca gcgagatttc ttcggtccac
      481 ttcgttaccg tcgcatagga ttgagttgat ctaaagaagt ggtgaacctg agaagtggat
      541 cgagtgtggc cgacgggtaa aagcagaact ggcaccgagt cggagtacgc ggtcgtaatg
      601 cctgagtagg cgttttctgc gccgggaccc gcctgcatcg taaacacgcc aatgcgccgg
      661 ccgtttaaaa cccgggtata accgtcggca atgcccactc cgactctttc ttgacggcag
      721 atcaccggct tgatccccgc cgccgcggca gcctcgatca gggtattggt tggatagcag
      781 gcaagaaagt ccacaccttc gcgcttgagg atttggctaa tagcatcgac aactcgcatg
      841 gttcacctcg tttgaatcag gaaggaactg aacgttcaac acggttcaaa aaagtcacta
      901 gttcgagctc agtttatctg aatcggttgt ttgcggatca gaatccaacg gcgcctctgt
      961 tccaccgtgc tgcggtggtg gcattgaggc gcagccgtga tagccgccag tcgtggacgc
     1021 tatccactcc tggatgtttt ttcaactggc agtttacttg atgtcgatcg gaaccaagca
     1081 ctcaatcatt cggctgcgtc cgccccacga agggagcgga tctgctatga gccaaattga
     1141 attcgccagt aatagttcgc gatgccaatg tactcgtgca acgtggcgta tatgccgcgg
     1201 gcggcgccct cgcccgggaa aaaatccagt ggagtgagac gaagtttgcc gagcgagtaa
     1261 tcccccggcg ccgcaagcgg ttccggagct ttggctttga acgccagcat gctgcgcggc
     1321 atgtgcagtg ctgaggttac cagcaagtag cgcttccaac cgcgccgttt gagaagcttt
     1381 tgcacttcaa ttgcgctctc gaaggtgtca cgcgatttgc cctcgccaat gacatggtgt
     1441 ttgggcacgc cccagcggat cagatactcg cggccgattc ggttctcgtc cttgtccggg
     1501 gtgaacggat tgacgtggcc gccggataga ataatcggcc taggttggat ccgataaaga
     1561 cgccaggctt catcgagccg ccggaacata tgctcgtcaa tagtcgagaa aggaatcagc
     1621 ccttcgggcg ggatcatacc gccagtgagg accacgatgg cgtcgtaaga gagagcgcct
     1681 ggatcgagca gcggccggta gcgagattca agcggatagc gcagcgcata acctaccaag
     1741 ccgctgtgca acgcggtgat cagcacgaaa gtcagcagaa gtaatttgcg cgcccagcgc
     1801 cgacgccaga agatcagcac cgccactagc atcagcagcc atggagttgg cggtagaacg
     1861 agagctttca aaaactgctt cagtacgaac atcgaaggtg atgacaaagc cgaaaaggaa
     1921 cggatcgatc aagcagttgg aacgagcttg tcgatataga aaagatttca actgacgggc
     1981 cgatcatcgc aacggctaaa ctattcggca tccggttggt tttccggcgc ggcgtcggcg
     2041 aacgcgggaa gggacataca ggcagcttca attgcgagca gctttgggta cgctgagaga
     2101 tcaacaccga agcggcgcgc attgataagt tgcggcacca ggcaaagatc ggccatagtt
     2161 ggtgtttggc cgaagcaaaa atctccctct ttggtcaccg agactatcat ccgttccaac
     2221 gctaaaaatc caagattgat ccaatggcgc gcccaacgga tcgtgtcttc atcggattgt
     2281 ttgtgatcgc ttttgagatg attgagcaca cgaaggttct gaatcgggtg cacctcgcag
     2341 gcgatcacta gtgccatgct gcgcacgacc gcgcggtcgg cggcatcgct cggaagcaac
     2401 gccggttttg gatagcgctc ctccagatac tcgataatag ccaaagactg tgaaaaggct
     2461 tgcccatcgt cttccagcgc cggcactaga gcttgcggat tgatcgcctt atacgcaggc
     2521 tttaactgct cgccgccgcc gcgccgcaag tgtatcgcgg cctgctcgaa ctccaaacct
     2581 tttagattaa gcgcgatgcg tacgcggtac gatgccgaac tacgaaaaaa tgtataaagc
     2641 ttcatttttg acctttgtca gcgagccgcc gaaaaatatt caccgcgatg gtagccgatc
     2701 acggatcttt tggattacaa ccagcctgca gagctgggac ctgcggcaat ttctacgctg
     2761 ctaactccac cgaagtgacg aacggtagac cggtgacaat ctcgcgtgcg ccggagtcgt
     2821 cgataaccag cagcgtgccg tcagaggtgc cggcaatcat gccgttgcca tccggcagcg
     2881 ggcacatgtg catgacgccg ccttttagat cgtcgatgat cttctcccaa gtggatcctt
     2941 tatcgcggct gcggaaaata gcggccatgg cgccctcttt acggatccat ccattgggcc
     3001 gcccttttgc gctaccaaga taaataatgc catcattgtc ttcagccacc gatgtgccgt
     3061 agtcgtggct ctgaggagtg atctcttccc aatgggcggc acgatcgaaa ctgcgaaacg
     3121 cggcctcgcc gcacgccgcg aacaccaatc ctgcagtatt tctcgacgcc agcacttcat
     3181 gaatgtccat gtcggtcagg ccttcgttgg cggggcgcca gttttgacca tcgtcgtcgc
     3241 taacgagcat tccgccgact tcgattccgg catagatacg attgggagaa gcgggatcaa
     3301 acgttaaata acgcgtccgt gattgcagtt caggactagg cggaaaattc gcgcccgcac
     3361 tgttaattcg tacgccttcc aactcttccc atccgccgtt cttgctgcgg taaaccgcag
     3421 atggaatcgt tccgaccata actcggccat ttaatgcgag tatggcaaac aacatcatct
     3481 tcgggacatt gggctgcgcg cgatgccaac tcgctcccgc gtcggcggtg taaaatagac
     3541 cggaatgctc ggtgcccgcg tagatcttct gcggattatc cggatcaacc gacatacagg
     3601 tcaggctcca gtatgacaga tcgcgtttgg tttgtttcca ctcccgaccg tcctgttcga
     3661 aacgatacca gccggacagt gtcgcgacgt gcaatgcttt tttcgcaccg ttagactccg
     3721 tcatggttat ctcctttagc ttgctgcacc gaattgagac acttctaact cgttcagggg
     3781 agaaatcgca accgcccatg tgctgagata tgaaataaca gggcagcagg gggcgcaggg
     3841 gccgcttgtg gtttgcgcgt tacgaggaac tttgatttat acggcacgcc ataacgtcgc
     3901 ctgaaatcga gcagttcaga aatagctttg gcagtacgat ctgaagtaag cgggaaatga
     3961 ccgttgcgac gagaccctct atggcgttcg cgcgcagcaa tctcgcttct tcatcgacgc
     4021 gggttctcgg tacgcaagtc tccgttagcc tcggttggtg tcgttggcca cgggaacttt
     4081 tcgcaccgac agcctgaggg tcgaagggct aatgtcctgc aaggtgagtt cctttggata
     4141 ttcgatgttt ttctctgaaa tggtgaaggt tcgtcgccca agctccgcca ttgaaacgtc
     4201 gattgcgacc ttgatcttgc tcgaattgaa caggtagaaa gcccgccggg gcccgcgaaa
     4261 tgtagcgttc accaccggcg gctctatgtc ttcgacttga agatcggggg gaagattctc
     4321 aacgctcacc ggaaccgtat agctgacctc gactgtcttt gatcccggca cgaagatgta
     4381 ccagaaccca attgccagtg cgatggcgat ggccttggcg acccagttct cacgaaaaag
     4441 cggtaacgcg aaattatttt gtgtcgccgg tggaaacttt gtttgcaaaa agttttcgag
     4501 aactgcgccc agctcttgta tgttctgcac tacgcgaagc ttcccgtccc gcgccaccga
     4561 gattgtgccg cgttcttccg acactacgat acacagggcg tctgtcagtt cggctaatcc
     4621 caaagccgcc gagtgccggg tgccgacgtt ggcgagctgt ctgagatctt tggagagcgg
     4681 caggtgagcc gcgaattttg agatgttatc ttggtcgacc ataaccgcgc cgtcatgccc
     4741 cggcgagtgg ggatcgaaaa tgctcttcag caacggcgca cttactttac cttccagagg
     4801 aatcccgccg gtgatgtgcc gttccagcgg atcgttgccg cgaatgacga tcaacgcgcc
     4861 gacgtgttcc ttagcgagat cggctacggt gcggaccaag acatctgaag tgctcgagcc
     4921 caaagtaggg gttcttttcc gcgccaggct ccagaccgcg atgcgctcga aaatctgccg
     4981 cagctcttct tgaaagatga cgacgataat gatcaagaaa atcgcgaaga aaccctggaa
     5041 tagccaggcg gtcagctgca gatcgagata gcgcgccaga atataaattg ccgcgagaat
     5101 gaaaattccg cgaatgatga acgcggcgcg ggtctgcttg agaaacgccg tggcggtgta
     5161 gagcaaaacc gcgacgaaga gaata
//
LOCUS       DASUTG010000024         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1041017, whole genome shotgun sequence.
ACCESSION   DASUTG010000024 DASUTG010000000
VERSION     DASUTG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041017"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /gene="atpD"
                     /locus_tag="VF089_00515"
     CDS             <1..1019
                     /gene="atpD"
                     /locus_tag="VF089_00515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6172464.1"
                     /translation="LETGIKVVDLLAPYARGGKIGLFGGAGVGKTVILMELINNVAMK
                     HGGFSVFGGVGERTREGNDLWLEMKESGVINKTSLVYGQMNEPPGARARVALTALTLA
                     EYFRDDEGRDVLLFLDNIFRFTQANAEVSTLLGRMPSAVGYQPTLATDLGELQERITT
                     THKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIAELGIYPAVDPLDSTSR
                     ILDPHVVGDEHYEVARQVQVILQRYKDLQDIIAILGMDELSEDDKLTVARARKIQRFL
                     SQPFHVAEAFTGTPGEYVELKDTIRGFKEIVDGKHDDIPEQAFYMVGAIEKAVEKAKK
                     MAAQ"
     gene            1099..1515
                     /locus_tag="VF089_00520"
     CDS             1099..1515
                     /locus_tag="VF089_00520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6172465.1"
                     /translation="MADKIRLKVVTPGRLLLDEEVDEVTAPGVKGEFGVLPNHISFLT
                     LLEPGEMSYKQGAIRRFLALSGGYAEVLDNVMTILAPAAEFGLEIDVNRARAAREKAE
                     RRLSETQYEDKSFATAQEALRKSLVRLQVAGKEARK"
     gene            1540..2673
                     /locus_tag="VF089_00525"
     CDS             1540..2673
                     /locus_tag="VF089_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6172466.1"
                     /translation="MAKAATKEPVITMWSKEFTYDRWMKSQGIPIHTGYYIEDLRKVE
                     LEWWEERGCRAAFIQLNGQEGVTASRVSEVPAGKSLPPVKFALDEIVYVLSGRGLTTV
                     WAGEGKAKKSFEWQEHSMFLVPRNHWHVFSNMQGDKPVRLFHYSYMPIAMSALPEPDM
                     YFNNPYEPKVSVGDGDFYSEAKLVQAKDADEGLGMRAFWYGNFFPDMRAWNKLDANER
                     RGAGGKSVFMQFPNSELTAHMSVFAPQTYKKAHRHGPGRAIIIPAGEGYSIMWEEGKE
                     KIVCPWHECSMVTPPDRWFHQHFNAGGDMARYLALHPPMQFHGHAEKVEDRAKDQIEY
                     PNEDPFIRRKFEEELAKRGIKSIMPDECYTNPDYEWSKAMGKQ"
     gene            2776..4293
                     /locus_tag="VF089_00530"
     CDS             2776..4293
                     /locus_tag="VF089_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6172467.1"
                     /translation="MHDDIYDHDHPDNFVIKEDEIVTDIEGLEMFSLKSVGIDIGSST
                     SHLIFSQITLRREGASLSGRFKVTNREVLYRSPIMLTPYVSATKIDTDKVNAFIHSAY
                     KEAGLTPEDVDTGAVIITGEALKKENAQPIVEAFAKYSGKFICAAAGHNHEALLAAYG
                     CGAVDLSKTEHKTVLNVDMGGGTTKFSLVEDGVVSQTASINIGARLIAFDEKDTITRI
                     EDAGRMMMNEIGHTVALGERITEQQKEDFGAYMARILFEIIEQGPTSGMAKRLMVTPP
                     FVNYRGFKQIHHLVFSGGVSEHVYDRDSKAYGDIGPVLGSNMREYLKKLPKEVLREPI
                     EGIRATVIGAGEYTIQASGNTSYVSNLEALPVHGLKVIQTALRKDVSVETALRQALAK
                     FDLPHFKSGLALSLTIDGQPDYQSVRKLAEGIAEVLRDSEDSKCPLYLTLDVDIAKSL
                     GGILRDELKVDRDIIAVDGIEVGDLDYIDIGECLGITEVIPVTVKSLMFPTTSSD"
BASE COUNT         1123 a         1118 c         1283 g         1046 t
ORIGIN      
        1 ctctggagac gggcatcaag gtcgtcgact tgctggctcc gtatgcccgc ggcggaaaga
       61 tcggtctgtt tggcggtgcc ggtgtcggca agaccgtcat tttgatggaa ctcatcaaca
      121 atgttgcgat gaagcatggc ggattttccg tgtttggcgg cgtcggtgag cgcacccgtg
      181 agggtaatga cctatggctg gagatgaagg agtccggagt catcaataaa acctccctgg
      241 tttatggaca gatgaacgaa cctcctgggg ctcgcgctcg agttgctttg acggcattga
      301 cgttggctga gtacttccgc gatgacgagg gccgtgacgt gctcctattc ctcgacaaca
      361 tcttccgctt tactcaagct aacgccgagg tttcaacact tttggggcgc atgccttcgg
      421 ccgtcggcta tcaaccgact ctcgccaccg acttgggcga actccaagag cgtattacga
      481 caacccacaa gggatcgatc acatcggtac aggcgatcta tgtgccggca gacgatttga
      541 cggacccggc gccggcgacc acctttgcac atctggacgc gaccaccgtt ctttcccggc
      601 agattgccga gttgggaatt tatccggcag tggatccgct cgattcgaca tcgcgaatat
      661 tggatcccca tgtagtgggt gacgaacatt atgaggttgc gcgccaagtc caagtcatct
      721 tgcaacgata taaggattta caggatatta ttgcgatctt gggcatggat gagctatccg
      781 aagacgataa gctgacggtg gcccgggccc gaaagatcca aagattctta tcccaaccct
      841 tccacgtcgc tgaagcattt accggtacgc ctggtgagta cgtcgagttg aaagatacta
      901 tccggggttt taaggagatt gttgacggca aacacgatga tatcccggag caggcgttct
      961 atatggtcgg tgccatagaa aaagcagtgg aaaaagcaaa gaaaatggca gcgcaataac
     1021 agctgctgcg attaggtaca gaagggcgcg gcgcttcgtt gctgttcgcg gccgctttga
     1081 ctgattactc gacggatcat ggcagacaaa attaggctta aagtggtaac gcccggccgg
     1141 ctccttttgg atgaggaggt ggatgaggtt actgcgcccg gagtgaaggg ggaattcggc
     1201 gtgttgccta atcacatttc gttcctgacc cttctagaac ccggtgagat gagttataaa
     1261 caaggagcaa tcagacgatt tctcgccctt agcggcggat atgccgaggt attggacaat
     1321 gtcatgacta tcctggcgcc ggcggctgaa tttggtcttg agatcgatgt caatcgagcc
     1381 cgtgccgcga gagagaaggc cgagaggcga ctaagcgaaa cgcaatatga agacaagagt
     1441 ttcgctacgg cccaagaagc cctgcggaaa tccctggtgc gattgcaggt cgccggcaaa
     1501 gaagcgagaa aatagcaggg aaagaaggag taatatttta tggctaaagc cgctaccaaa
     1561 gaacccgtca tcaccatgtg gagcaaggaa ttcacctatg atcgctggat gaaatctcag
     1621 ggaattccca tccacaccgg ctactacata gaagatttgc gaaaagttga gttggaatgg
     1681 tgggaggagc ggggttgccg ggcagcattt atccagctca atggtcaaga aggcgttacc
     1741 gcatctcgtg tgtccgaggt tcctgcgggt aagtcgctgc cgccggttaa attcgcctta
     1801 gatgaaatcg tttacgtttt gtcaggtcgc ggcttgacta ctgtgtgggc cggcgaaggt
     1861 aaagcgaaga agagtttcga gtggcaggaa cattcaatgt tcctggtacc gcgcaatcac
     1921 tggcatgtct tcagcaacat gcaaggcgac aagccggtcc ggctgtttca ctatagttat
     1981 atgccgatcg ctatgtctgc gctgccggag cctgacatgt atttcaacaa tccgtacgaa
     2041 cccaaggtta gcgtcggcga tggtgatttt tattccgaag caaaattggt tcaggccaaa
     2101 gatgcggatg aggggctcgg aatgcgagca ttttggtatg gaaatttctt tcccgacatg
     2161 cgtgcctgga acaagctcga cgccaatgaa cggcgcggcg ccggcggcaa gagtgtcttc
     2221 atgcagttcc ccaattcgga attaaccgcg cacatgtcgg tcttcgcgcc gcaaacatac
     2281 aagaaagcgc accggcacgg cccgggccgc gcgatcatta ttccagccgg cgaaggttat
     2341 tcgattatgt gggaggaagg caaagaaaag atcgtttgtc cgtggcacga atgcagcatg
     2401 gtgacgccgc ctgacagatg gttccaccag cacttcaatg ccggtggtga tatggcgcgc
     2461 tatctggcgc ttcatccgcc catgcaattc catggtcacg cggagaaggt cgaagatcgc
     2521 gcgaaagatc agattgagta tccgaacgaa gatccgttta tccgccgcaa gtttgaagaa
     2581 gaactcgcca agcgcggcat caagagcatt atgccggatg aatgttatac caatcccgac
     2641 tacgagtggt ccaaagcgat gggtaagcaa taaagctttt aagatcgttc gacataaaac
     2701 aagaatcccc gccgttgcgc ggggattctt gtttctactc gataagatgg aagactgatt
     2761 ttacaaggcg gaactatgca cgacgacata tacgatcatg atcacccgga caatttcgtc
     2821 attaaagaag atgaaatcgt caccgacatt gaaggactgg aaatgttctc gcttaaaagt
     2881 gtcggcatcg atatcggttc ttcgacttcc catctgattt tttcgcagat cacactgcgc
     2941 cgtgagggcg catctctttc aggcaggttc aaggtaacga atcgggaagt tctttatcgc
     3001 tcgccgatca tgctaacgcc ctatgtgtcc gccaccaaaa tcgatacgga caaggtaaat
     3061 gccttcattc acagcgcgta caaagaagcc ggcttaacgc cggaagatgt cgatactggc
     3121 gcggtaatca tcaccggaga ggcgctcaaa aaagaaaacg ctcaacccat tgtcgaagct
     3181 tttgccaaat attcgggtaa gtttatctgc gctgccgctg gtcataatca cgaagcgcta
     3241 ctagccgctt acggttgtgg tgcggtggac ttatccaaaa ccgagcacaa gacggtgctg
     3301 aacgtcgata tgggcggcgg aacgacgaag ttttccctgg ttgaagacgg cgtggtttcg
     3361 caaacagcct cgatcaacat cggcgcacga ctgatcgcct tcgatgagaa ggatactatt
     3421 actcgcattg aggatgctgg ccggatgatg atgaacgaga tcggtcacac ggtagcccta
     3481 ggcgagcgaa tcactgagca gcagaaggag gacttcggtg cttacatggc gaggattctt
     3541 tttgaaatta tcgagcaagg accgacatcg ggaatggcca aacgactgat ggtcaccccg
     3601 cctttcgtca attatcgagg gttcaagcag atccatcatc ttgtcttttc cgggggtgtc
     3661 tcggagcacg tctatgatcg tgactccaaa gcttacggcg atatcggtcc ggtactcggc
     3721 agtaatatgc gtgagtacct gaaaaagctg ccgaaggaag tgctccgtga gccgattgaa
     3781 gggattcgcg ccactgtaat cggcgccggc gaatatacaa ttcaagctag cggcaacacg
     3841 agctacgttt cgaatctcga agccttgccg gtgcatggct tgaaggtgat tcagacggca
     3901 ttgcgcaaag atgtctctgt ggaaacggca ctcaggcaag cgctggcaaa gtttgacttg
     3961 ccgcatttta agtcaggtct ggctctgtct ctcacgattg atggacaacc ggactatcaa
     4021 tcggttcgca agttagccga aggcatcgcc gaggttttgc gggattccga agatagcaag
     4081 tgtccacttt atctgacgct tgatgtagat atcgccaaat cattaggcgg aattttacga
     4141 gacgaactca aggtcgatcg cgatatcatt gcggtggatg gtatagaggt tggcgatttg
     4201 gactatatcg atattggtga gtgcttgggg attaccgagg tgatcccggt caccgtcaag
     4261 tcgttgatgt tcccgaccac cagcagcgat taagttcgcc gtttgtagag aaccttcgcg
     4321 tctgatcttc tgcagcacca gagaattcaa acgtagccgg acaaagccgt tcattgcaga
     4381 gaggccggca gcgaagcctg cgcattcgat cggcgagcct atcacgttgc cgctcgcgat
     4441 cacgcaactc agttgtagtg gaagatttct tgcgcgccaa gctctgctga tgttctcgcg
     4501 gatggtgtcg tccgctccag ccgccccggt gatggtcgaa ccaatctgac agcaacacgt
     4561 ctctccttcg
//
LOCUS       DASUTG010000025         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_893174, whole genome shotgun sequence.
ACCESSION   DASUTG010000025 DASUTG010000000
VERSION     DASUTG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893174"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            708..1139
                     /locus_tag="VF089_00535"
     CDS             708..1139
                     /locus_tag="VF089_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6172468.1"
                     /translation="MAERDWDIFIWSRANELLHRAERIQRNFIQIAAATQYRSSQGRA
                     PSWEPPVNVIETDESLWVISAIPGVSSDQVQVRIDGNELVITGHRPLPKCCTDGDLKI
                     WEIPLGRFERRLRVIAGETPLLLGEITLKEGLLIIQLRKHS"
     gene            1136..3547
                     /gene="lon"
                     /locus_tag="VF089_00540"
     CDS             1136..3547
                     /gene="lon"
                     /locus_tag="VF089_00540"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6172469.1"
                     /translation="MKAEPAETPKVKPRINIPEGALVILPLRNAVLFPSTIMPLVVGR
                     PSSLQAIEETVRQQLPVGFIGQRDPAVETPKPADLYNIGTAADILRMFSLPDGQRQII
                     AQGRRRFEIAEFLQIDPLLIARVTMVEETIPQTKEFEARIVNLRQEASRALSLLPEPM
                     NELKTTIERIDDPLMLIDMIASTLDIPTPEKQELLATLDPVTRAQRVSEKLARQIEVL
                     ELSKKISEETKDSMTKAQRQYFLREQLKAIQKELGEEDGKGVEAEDLRQKIFDAKMPP
                     EVEKEALNELNRLQRMQEGGPEYSSLRTYFDWLVELPWSVLTKEEIDLPKAREILDAD
                     HYGLEKVKKRIIEFLAVRKLKPEGKSPILCLVGPPGVGKTSLGQSIARAMSRKFVRQS
                     LGGVHDEADIRGHRRTYIGSLPGNIIQGIRKAGSRNPVFMLDEIDKLSASFHGDPSAA
                     LLEVLDPSQNSTFQDHYLGVPFDLSQVLFIATANVLDTIPGPLRDRMEILEVPGYIED
                     DKLAIAKGFLIPRQTVENGLKPEQIHFTDEAIREIIRSYTREAGVRQLERELGAVCRS
                     VATRVAEGITEPITIDAEAIVNYLGQKKFFNEIALRTSLPGVATGLAWTPVGGDILFV
                     EVTKMPGEGRLILTGQLGEVMKESAQAALSLVKSRAEALGIDPEVFKKNDLHIHLPAG
                     AIPKDGPSAGTTLFVALVSLLTGRRVSKDVAMTGEISLRGLVLPVGGIREKMLAAKRA
                     GITHALLPDLNRRDIEEIPPEARAGMHFDFLNTVDDALLLALESNGAGSGFDAAERPA
                     ADAIP"
     gene            3757..>4132
                     /locus_tag="VF089_00545"
     CDS             3757..>4132
                     /locus_tag="VF089_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6172470.1"
                     /translation="MKFLSSQISYFFSEPGTRANISALLKYLVLLILIIVLYSVLFHV
                     IMLYAEGRYHSWITGVYWTLTVMTTLGFGDITFASDLGRFFSIIVLLSGIVLLLIVLP
                     FMFIRLFYAPWLEAQVRFRAPRE"
BASE COUNT          957 a         1125 c         1146 g          904 t
ORIGIN      
        1 tagggaagaa tgttgcttcg ccaacgatca tcgggtcgat tttggtgacc tgggtaggac
       61 ccgcacaacc cagaacaaac aacaaagtca aaaacagaat ccgcatcatc tacattcaat
      121 gtagcaattt ctgcaaagcg caacaagttg cttcagcgag cgttaccgcc gggcatctcg
      181 gcttatgtag ctgcgcattt caggctttgg ctacaaaatg tatcgtgtag agttgtatcg
      241 gcatcgcgca tgctgactcg cacccgaagg caactagaag cagcggagat tcgatcgtct
      301 ctcttagttg cactggagcc taacacctgc cgtgcccgag gcggatttca gatgatttac
      361 aagcaagcac ttgattgtag cctgtcagcg acaaaccaac ttatcggagc cttgagaccg
      421 ctgctggcca ttatcttctc tgccgctcgc aaattgtaag cgccgctgcc aagaattagc
      481 acgtgctgtc accaactctc ttgggtccca gagggtgccg ctgcaaatat aaccgtcgcc
      541 tttcttgaca ttgattgccg ccattcctac tgttgataca gtagcttcgt cgtcgcggga
      601 ggggtgtgac aatgacaagg ggcattgcac gcttgaatcc cactcaggat catgatcctt
      661 cattccgcta ctgatttgcc cctcagttgt tggaggcagc gattaaaatg gctgaacgcg
      721 attgggatat atttatctgg tctcgagcga acgaattgct ccaccgagca gagcggatcc
      781 agcgcaattt catacagatc gctgcggcga cgcaatatcg gtcatctcag gggcgagctc
      841 cgagctggga gcctccagtc aacgttatcg aaaccgatga aagtctctgg gtgatttctg
      901 ccattcccgg agtttcctcg gaccaggtac aagtgcgaat cgacggcaac gaactggtga
      961 tcaccggtca tcggcctctg ccgaagtgtt gcaccgatgg ggatctaaaa atatgggaaa
     1021 ttccgctcgg tcggttcgag cggcgtctca gagtgatagc aggcgaaacc ccgctacttc
     1081 ttggcgagat tacattgaag gaaggcctgc taattattca gctaaggaaa cattcatgaa
     1141 agctgaaccg gctgaaactc ctaaggtcaa accccggatt aacatcccgg aaggggcact
     1201 ggtcatcttg ccgctgcgca acgcggtgct ctttccctca acgatcatgc cgcttgtggt
     1261 tggacgcccg tcaagtctgc aggccattga agagacggtg cgacagcagc tgccggtagg
     1321 cttcatcggt caacgagacc ctgcagtgga aacgccaaag ccggcagacc tgtacaacat
     1381 tggcacggca gcggacatac tgagaatgtt cagtctcccg gatgggcagc ggcaaatcat
     1441 tgcccaaggc cgccgccgct tcgaaatcgc cgagtttctt cagatcgatc ctttgttaat
     1501 cgcgcgggtt acaatggtgg aggagacgat cccgcagacc aaggagttcg aggcaagaat
     1561 cgttaatctg cgtcaggaag cttcgcgagc gttgtctttg ctgcccgagc cgatgaacga
     1621 gttgaaaacg accatcgaga ggatcgacga tcctttgatg ttgatcgata tgatcgcctc
     1681 gaccctcgac ataccgacac ctgagaagca ggagctactc gcaactctgg acccggtaac
     1741 ccgcgcccaa agagtgtcag agaaattggc gcgccagatc gaagttctgg aactaagcaa
     1801 gaagatcagc gaagaaacca aggactcgat gaccaaggcg cagcgtcagt attttctccg
     1861 tgagcagctc aaggccattc aaaaagagct tggtgaagag gacggcaaag gcgtggaggc
     1921 cgaggatctg cgccagaaga ttttcgacgc gaaaatgcct cccgaggtgg aaaaggaagc
     1981 gctcaacgag ctcaaccgct tgcagcgcat gcaggagggc ggacccgagt actcgtcgct
     2041 acgcacctac ttcgactggt tggtcgagct gccgtggagt gttctcacca aagaagaaat
     2101 cgacctgccg aaggcgcgtg agattctcga cgctgatcat tacggcctgg agaaggtaaa
     2161 aaaacgtatc atcgaatttc tcgcagtgcg taagctcaag ccggagggaa aaagtccgat
     2221 cctgtgctta gtcgggcctc cgggagtcgg caagacctcg ctcggacaaa gcatcgcccg
     2281 ggcgatgagt cgcaaattcg tgcgccaaag cttgggcgga gtccatgacg aggcagatat
     2341 ccgcggccat cgccgcacct atatcggttc gctgccgggc aatatcattc aaggcattag
     2401 aaaggccggc agcagaaatc cagttttcat gctggatgag atcgataagc tcagcgccag
     2461 tttccacggc gatccttcgg cggcgttgtt ggaagtcctt gatccgtcgc aaaacagcac
     2521 gtttcaggat cactatcttg gcgtgccctt cgatctgagc caggtactat tcatagctac
     2581 ggcaaacgtc ctcgatacca ttcccggccc gctgcgcgat cgcatggaaa tcttggaagt
     2641 gccgggctat atcgaggacg acaaattagc catcgccaaa ggctttctta ttccaagaca
     2701 aacggtcgag aacgggctca aaccagaaca gattcatttc accgatgagg ctatccgcga
     2761 gatcatcaga tcgtacactc gagaggccgg cgtgcgccag ctcgagcgcg agttgggtgc
     2821 ggtgtgccgg agcgttgcga cgcgggtggc cgagggtatc accgagccga tcaccattga
     2881 tgccgaagcg atcgttaatt acctgggcca gaagaagttt ttcaacgaga ttgcgctccg
     2941 caccagctta ccgggagtgg ccacgggctt ggcttggacg ccggtaggcg gtgacatttt
     3001 gtttgttgaa gtgaccaaaa tgccgggaga gggtcgattg attttgaccg gccagttggg
     3061 cgaggtcatg aaagaaagcg cgcaggccgc tttgagtctc gtcaaatcac gcgccgaagc
     3121 gctgggcatc gatccagagg tgttcaaaaa gaacgatttg catatccatc tccccgctgg
     3181 ggccatccca aaggacggtc ccagtgccgg cactacgctg tttgtcgctt tggtttcact
     3241 tctgactgga cggcgagtca gcaaagacgt cgccatgacc ggcgagatca gcctgcgcgg
     3301 cctcgtcctg ccggtcggag gaatcaggga gaaaatgctg gcggccaaac gcgccgggat
     3361 cacacatgcc ttgctgcccg acctgaaccg cagggatatt gaagagattc caccggaagc
     3421 acgcgccggg atgcattttg attttctaaa caccgtcgat gatgccttat tgctggcgtt
     3481 ggaatccaac ggcgccggca gcggcttcga tgccgccgag cggcctgcgg ccgacgctat
     3541 accctgacga cgctcgttcc ctgcgcgggc atttgtcaag actcgactgg atgcacgggg
     3601 caactcggaa ggctgaggtc tcggagttcg ttggattcgc taatctgtta gctcatcctt
     3661 cagcgttgca acgctttcag gtaagccgtt ccaccgccgt acgtgcccaa cccctcttaa
     3721 tcctcgcctc aaccatttga gccgcccagc cacaagatga aatttctgag cagccagatc
     3781 tcctacttct tcagcgaacc cggcacgcgc gcgaatatca gcgcacttct caagtactta
     3841 gtgttgctga ttctcatcat cgttctctat tccgtgctct ttcacgtgat catgttgtac
     3901 gccgaaggac gttaccattc ctggatcaca ggagtgtatt ggacgctgac ggtgatgacc
     3961 acgttggggt tcggcgatat caccttcgcc agcgatctcg ggcgattttt cagcatcatc
     4021 gtgctgctgt cgggcattgt actgcttctg atcgtcctgc cgtttatgtt catccggctc
     4081 ttttatgcgc catggcttga agctcaagtc cgttttcgag cgccccgaga gg
//
LOCUS       DASUTG010000026         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1634399, whole genome shotgun sequence.
ACCESSION   DASUTG010000026 DASUTG010000000
VERSION     DASUTG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634399"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF089_00550"
     CDS             <1..425
                     /locus_tag="VF089_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172471.1"
                     /translation="KAVEAGGHVLFNLQETIIPYQHTGLVTTRNFISSRPDITRRVVK
                     SIVEGAALVRKEPAIAKRALSARLHIKDGRELQETYQLLAGFTRPKPYPNLDGFRAIL
                     SSLTNRMPSAKTADPKDFSEPKFIEELDQSGYIDSLYK"
     gene            397..1404
                     /locus_tag="VF089_00555"
     CDS             397..1404
                     /locus_tag="VF089_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172472.1"
                     /translation="MVISTVFTSKRGIGALSLILLNVLVYGVASAADKIRIGYSGVTV
                     SNAMLWVTDEGKLFQKNGIDPQILYLRTTLGQTAMIAGEVDMCVYSGSLLSSARLQGA
                     DVVMVTSFLNRPLYRLVVRPEIRTVADLKGKRLGITRFGTVTDSMSRLLVGKMGLDPD
                     KDVSYIQVGDVPILLASLTTGKTIDGAIIQPPYYLKAVASGMRVLVNMQDMDIPVQQT
                     GLNTTHKFIAKNPDLVRRVVKSLIEGVHLMRSDPTVAKRAISKRMQIKDEKEIEDTYL
                     LLKSFVPIKPYPTLDGFKTILDDLSKRIPAAKTANPKEFVDTRFIEELDRSGYIDSLY
                     K"
     gene            1409..2311
                     /locus_tag="VF089_00560"
     CDS             1409..2311
                     /locus_tag="VF089_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6172473.1"
                     /translation="MEQYDIVIVGGGMAGLSAALYGGWLGHKVLLAERQMFGGQIVNA
                     DQVENYPGFSEGILGADLVSQVRTQALKFGAKMQYVEITAIEKHHEAFVLESNEDTYG
                     AQSVIVATGGKPRHLGIKGEVELEGSGVSRCATCDGAFFADKPVAVIGGGDTALDEAF
                     FLAGIASKVTIIHQTEQPTASAALVTRARGNNKIEWISRASVTEIIGSESVEGLRLSQ
                     GEAIVTAAVFIAIGFEPETALLTSLIEIDPMGHAPVDLHMQTSMPGLFAVGSARQGSS
                     GQISSVTGDGVTAAIFSHRYISTR"
     gene            2418..>2811
                     /locus_tag="VF089_00565"
     CDS             2418..>2811
                     /locus_tag="VF089_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6172474.1"
                     /translation="MISGALEGFTCIDVSGHVAGPYAGSLLGDLGCDVIKVELPGGGD
                     THRGRNPKYEGYGPSFRALNRNKKSVTLDLRQKKGREILLKLLTTADIFLENFRPPTR
                     SKLALDYPELAKINSKLVHCSISGYGQSG"
BASE COUNT          689 a          706 c          773 g          643 t
ORIGIN      
        1 gaaaagctgt cgaagccggt ggccatgtct tgtttaacct tcaagaaacg atcattcctt
       61 accaacatac gggcctcgta accacccgca acttcatcag cagccgacct gatattacgc
      121 ggcgcgttgt aaaatccatc gtggaaggcg cagcgctagt gcgaaaagaa ccggccatag
      181 ccaaacgggc cttgtcagcc cgtctgcaca tcaaggacgg aagggaattg caagagactt
      241 atcaactgtt ggccggcttc actcgaccga aaccctaccc gaatttggat ggattccgcg
      301 cgatcctgag cagtctgact aacagaatgc cttccgctaa aaccgctgat cccaaggatt
      361 tttccgagcc gaaattcatc gaggagcttg accaaagtgg ttatatcgac agtctttaca
      421 agtaaacgcg gcatcggcgc actatctttg attctgctta acgtgctggt gtacggagta
      481 gcaagtgccg ccgacaagat ccgcattggc tattctggcg tgacggtcag caacgccatg
      541 ttatgggtca ccgatgaggg caagttattt cagaaaaatg gcattgaccc gcaaatcctt
      601 tacctgcgga ctaccctcgg acagacggca atgattgcag gtgaagtgga catgtgcgtg
      661 tattccggaa gtttactttc atccgctcga ctgcagggcg ccgacgtggt catggtgacc
      721 agctttctca acaggccact ctaccgactt gttgtgcgcc cggagatcag gactgttgcg
      781 gatctcaagg gtaagagact cgggatcacg cgtttcggca cggttacgga ttcgatgtct
      841 cgactcctgg ttggaaaaat gggattggat ccagataaag acgtgtccta tattcaagtt
      901 ggcgatgtcc cgatccttct agcatccctc accacgggca agaccattga cggtgcgatc
      961 attcagccac cttattatct caaagccgta gcgtccggca tgcgagtttt ggtgaatatg
     1021 caggatatgg atattcctgt gcagcaaacc ggcctgaaca cgactcataa atttatcgcg
     1081 aaaaatcctg acctcgtgcg ccgggtcgtg aagtcgctga tcgaaggggt tcatttgatg
     1141 cgtagtgatc cgactgtggc caaacgtgca atatcgaagc gcatgcagat caaagacgaa
     1201 aaagagatcg aggatacgta cttgctgctc aagagtttcg tgccgatcaa gccctatccg
     1261 acgctggacg gttttaagac aatcttggac gacctgtcca aaagaattcc cgcggcaaaa
     1321 acagccaacc cgaaagaatt tgtcgatacg cggtttatcg aagagcttga ccgttccggc
     1381 tacatcgaca gcctctacaa atagcgtcat ggaacagtat gacatcgtaa tcgtcggcgg
     1441 gggcatggcc gggctgagcg cggctttgta tggtgggtgg ctgggtcaca aagtattact
     1501 cgccgagcgg caaatgtttg gcggacaaat cgttaatgcc gatcaggtag agaactatcc
     1561 gggcttctct gagggaatac tcggtgctga tttggtgtcg caggtgcgaa cgcaagcgct
     1621 caagtttggc gcaaagatgc aatacgttga gatcaccgcg atcgaaaagc atcacgaagc
     1681 gtttgtcctt gaatcgaacg aagatactta tggcgcgcaa agtgtgatcg tcgctacggg
     1741 cggaaagcct cgccacctcg gaatcaaagg cgaggtcgag ctcgaaggtt ccggcgtttc
     1801 ccgctgcgcg acttgtgatg gcgccttctt cgcggacaaa ccggtggcgg tgatcggcgg
     1861 cggagacaca gctttggacg aagcgttttt tcttgcaggt attgcaagca aggtgacgat
     1921 cattcatcag actgaacaac cgaccgcgag tgccgcgctt gtgacgcgcg cgagaggaaa
     1981 taacaagatc gaatggattt cccgcgcctc ggttacggaa atcatcggta gcgaatccgt
     2041 tgaaggtttg cgattgagcc aaggtgaagc aatcgtcacc gcagccgtgt tcattgccat
     2101 tggttttgaa ccggaaacgg ccctattgac aagcctcatt gagatcgacc ctatgggtca
     2161 tgcgccggtt gatctccata tgcagacgtc catgcctggt ttattcgcgg tcgggtccgc
     2221 tcggcaagga agctcgggac agatctcatc cgtaacgggc gatggggtta cagccgccat
     2281 cttttcccat cgttatattt ccacaagata gaatgtgaga atttgctagt cttaatcgat
     2341 cctgcttgga atcgcagtgg aaaacggtgt tcgattgcga taagtgaatc gcttgagatt
     2401 gaggttttga cacgcgcatg atttctggag ccctggaagg tttcacatgt atagacgtta
     2461 gcggccacgt cgccggcccg tacgcgggaa gcctcttagg cgatctcggc tgtgatgtca
     2521 tcaaagtgga actgcctggt ggcggcgata cccaccgcgg gcgcaatcca aaatacgaag
     2581 gctatgggcc gagctttcgc gcgctcaatc gtaataagaa aagcgtcacc ctcgatctgc
     2641 gacagaaaaa aggccgagag attctgttga agctgctcac cacggctgac atttttctgg
     2701 aaaacttccg ccctccaacg aggagcaaac tagcgctgga ctatccggag ctggcgaaaa
     2761 taaattccaa gctggttcac tgttcgattt cgggttacgg ccagagtggt c
//
LOCUS       DASUTG010000027         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_448538, whole genome shotgun sequence.
ACCESSION   DASUTG010000027 DASUTG010000000
VERSION     DASUTG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448538"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VF089_00570"
     CDS             <1..177
                     /locus_tag="VF089_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEX6172475.1"
                     /translation="CRTIAIEVGMQNSGLGVALARKHFADVLVALPAAIFSVVQNITG
                     PAVANFWSRKHAGP"
     gene            586..1026
                     /locus_tag="VF089_00575"
     CDS             586..1026
                     /locus_tag="VF089_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6172476.1"
                     /translation="MTTLNMQGLSDKPKPVVGRKKWYFIMVTKILLVEDHPELRDILQ
                     GRIEGMGFTTITAKNGREGVDTAVAEKPDLILMNSIMPEMDGWEATKLLRSNPETKDI
                     PILAATALFRPSHLEAWLDVGCNDYILKPFTMDELSRKIRALIG"
     gene            complement(1285..2268)
                     /locus_tag="VF089_00580"
     CDS             complement(1285..2268)
                     /locus_tag="VF089_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172477.1"
                     /translation="MKPLLASAASGSPDGKKSRCIKLLAVALFVFSWVVPESGPAQPI
                     TTGAKPENPGKKAPPSEINYAVTEVFPLGDRYIGAFNRSRELALVGNFDGYFVDKKRR
                     VTDFECVSFSHGAAQHFTTPLGINNYGDIVGFCHDDIGPIESGFLRSKNGNLFQFTAP
                     DSTSTVAYGINDFGQIVGQSSGAFGAFSFIRSSDGQYRHISPPTIEAVVRAVAINNSL
                     KVVGHYSDINGQHAFFWDNGQFTELRVPGATGNSDVRQLNNNDQALMFATVDGVGSYY
                     LYDGGNYLRINSPEGYTWQTVNALNDNGELTGFAFDDQNDFFDIIAKPTKK"
     gene            complement(2652..3740)
                     /locus_tag="VF089_00585"
     CDS             complement(2652..3740)
                     /locus_tag="VF089_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6172478.1"
                     /translation="MLSESAQTRSQVKLWRPKRVLLTPAARELAHGRNIAEKAASWGA
                     EVVELNSNRLRGLVSDDRRRAYMDAKSTLAIVISPASKRKLQPIPPSADWRFDLAEGC
                     PAHCQYCYLAGSLSGAPVTRVYANIEEILDGLSEYVGRGHITSASKARAEEGTTYEAS
                     CYTDPLGIEHLTGSLAKAIRHFGDWKAPVQLRFTTKYDNVDSLLALAHSNHTRIRFSI
                     NSAPVARFEGGTAIMPARLNAMRRAALAGYPVGLTIAPIMPEGNWREAYGLLLRQAAV
                     ALAGLPNVDLTMELITHRFTPKSKAVLNEWYSSSRLDMDEDTRAKKRTKFGSLKYVYP
                     AHLMKELREFFEKTIAEHLPSARILYWT"
     gene            complement(3768..>4075)
                     /locus_tag="VF089_00590"
     CDS             complement(3768..>4075)
                     /locus_tag="VF089_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore photoproduct lyase family protein"
                     /protein_id="HEX6172479.1"
                     /translation="WEADYVELFKKLAGTLSSAAKEQLKAEVIFLTHNAALHEVNLRW
                     HHRAEDWLWRPELQQKKLSQNGMWNLRYKVHEKRRYLESFLDALRRTLPGCEVRYAF"
BASE COUNT          971 a         1052 c         1097 g          955 t
ORIGIN      
        1 tgccggacga ttgccatcga agtcggcatg caaaactcgg gtttgggagt agcgctggcg
       61 cgcaagcatt ttgcagacgt gctagttgcg ttgccggcgg cgatcttcag cgtagtgcag
      121 aacatcactg gcccggcagt agccaacttc tggtcgcgca aacacgccgg cccatagccg
      181 cggagtggaa agccggaatt caaagtaggg cggcagaagg agccctggga gtgaaggcat
      241 ctatcgagtg ccggctttgg ccgtcggatg gagaacatat tagccgaagt agccctcgat
      301 ggaaacgatg tccggtactg ttatgagatc ccgcgtcatt tggcttttac tatgcaaccg
      361 tgagccgcag tctcaagcaa gtcgaagaat caaaacaaaa ttgtcgcggg caggcgctaa
      421 agcggcgcgg atcccgcgcg gtaccggtga gcgacgtggc cgtctctcga attcagaggt
      481 cgtacccagg aaagcatttt aaatcttccg acgatccttc ctgatgttcg tgcggatccg
      541 agcaggggac gcgagtatac agtacggttg acgaacagtg attccttgac tacgctcaat
      601 atgcaagggt tgagtgacaa gcctaaaccg gtcgtcggca gaaagaagtg gtattttatc
      661 atggtcacta aaattctgct ggttgaagat catcccgagt tacgggatat ccttcagggc
      721 cgaatagaag ggatggggtt cactaccatc actgctaaga acggcaggga gggcgttgat
      781 acagctgttg cggagaaacc agacttgatc ctgatgaatt ctataatgcc ggagatggat
      841 ggttgggaag cgaccaaatt attgcggtca aatcctgaga ccaaggatat tccgatcttg
      901 gccgcgacgg cattgttccg cccgtctcat cttgaagctt ggctagatgt cggctgcaac
      961 gactacatcc tcaagccatt tacaatggat gaactctcaa ggaaaataag ggcgctcatt
     1021 ggatgagtgc cacggccggg agtcaaatca cgaaagcatc ctaattcccg ataatgactt
     1081 ccgcacatag taaggtcgtt cgaaggttac gacgcttttg cttgaaacga cccttccgca
     1141 gatccagtcg tattgacggt ttgccgcttc gctcacatca aagttttctc ggttgaggcg
     1201 cttggttcct cagatttcac atcagaagcg tcgctcggtc tagcgtctgg ctacaggttc
     1261 gcttccaact ttatcgcctg tgccttattt cttagttggt ttagcgatga tgtcgaaaaa
     1321 atcattttga tcgtcgaaag caaaaccggt tagttctcca ttatcgttga gggcgtttac
     1381 agtctgccaa gtgtagcctt caggactgtt tattcgcagg taatttccgc catcgtataa
     1441 atagtaggat ccgacaccat ccactgttgc aaacatcagt gcctgatcat tgttgttgag
     1501 ttgccggaca tcgctgttac cagtcgcacc gggcactctc agttcagtaa actgtccgtt
     1561 gtcccagaag aatgcatgtt gaccgttgat gtccgagtaa tggccgacga cttttaaact
     1621 gttgttgatc gcaacagccc ttaccaccgc ttctatcgtc ggaggcgaaa tgtgtcgata
     1681 ctgaccatcg ctgctccgaa taaaactgaa agcgccaaaa gcaccggacg actgcccgac
     1741 gatctgtcca aaatcattta tgccatacgc gacggttgat gtggaatcgg gtgcggtaaa
     1801 ttggaacaga ttgccgtttt tactacgcaa gaaaccgctc tctatcggac cgatatcatc
     1861 atgacagaat ccaacaatgt cgccgtaatt attgattccc aaaggtgttg taaagtgttg
     1921 agcggctcca tgggagaatg atacgcattc gaagtctgtg actctgcgct tcttatcgac
     1981 aaaataaccg tcgaagttac cgaccagcgc caattctcgc gaacggttaa atgctcctat
     2041 gtatctatcg ccgagcggga agacctcggt aacagcataa ttgatctcgc tcggcggagc
     2101 cttcttgcct ggattctcgg gctttgcacc agtggtaatg ggttgtgctg ggccactctc
     2161 tggaacgacc catgagaaga cgaaaagagc tacagccaaa agctttatgc aacggctttt
     2221 cttgccgtcg ggcgatccgg aagcagcaga agcaagtagc ggtttcattt acacctccaa
     2281 ctcaacggtt gattatgggc tgacatggct ctcgtgcgcg agttctgcgg tccgggctgc
     2341 taagctccga catgcttaca agaacagccc atatattcca aagattgcca taaattgcct
     2401 aaaactgcat agcaccagta gcgctggagg gtcaatagga tttttgcgga gtgttgtagc
     2461 tagtcaaaac gaaagaggaa tttggatgcg ccgacggaga gactggtcaa caaccgggac
     2521 tactttcaac tgacgctgtt tctttctccc tagcaaagcg acgcatcgaa aacttctagg
     2581 agagaactct tcccggcccc agtccgatcg ccgacaaatc ccagcagtat ccgacgaggt
     2641 gaaatgacaa cttaggtcca ataaaggatt cgagcgcttg gcagatgttc cgcaatcgtc
     2701 ttctcgaaaa actcacgcag ctccttcatc aaatgggccg gatagacgta tttcaacgag
     2761 ccgaacttgg tgcgtttctt agctctggta tcctcgtcca tatcgagcct ggagctactg
     2821 taccactcgt tcaaaaccgc cttcgatttg ggcgtgaagc ggtgcgtgat gagttccatt
     2881 gtcaagtcga cgttgggtaa acctgcaagc gccacagcag cttgccgtaa caacaaccca
     2941 taagcctctc gccaattacc ttccggcata atcggcgcga tcgtaagccc aaccggatat
     3001 ccagcaagag ccgcgcgccg catggcgtta aggcgcgccg gcatgatggc cgtgccgcct
     3061 tcaaagcgcg ccaccggagc gctattgatt gagaaccgga tacgcgtgtg attgctgtgt
     3121 gcaagggcga gaagtgaatc gacattgtcg tacttcgtgg tgaagcgcag ctgcaccggt
     3181 gccttccagt cgccgaaatg acggatcgcc ttggcaagcg aaccggtgag atgttcgatg
     3241 ccgagtggat ccgtgtaaca cgacgcttca taggttgtgc cttcttcggc ccgcgctttg
     3301 gaagcggagg tgatatggcc gcgtcccaca tattcggaga gcccgtctag aatctcttct
     3361 atattggcat aaacacgcgt gaccggcgca ccgctcagcg aaccggcgag ataacagtat
     3421 tggcaatgag ccgggcatcc ttccgccaag tcgaagcgcc aatcggcgct cggcggaatc
     3481 ggctggagct tccgcttgga agcgggcgag atgacgatgg cgagtgtgga cttcgcgtcc
     3541 atatacgctc gtcgccgatc atcgctcaca agccccctca aacgattgga atttagctca
     3601 actacttcag cgccccaaga agcagctttt tcggcaatgt tccggccatg tgctagttca
     3661 cgagctgcag gtgttagcag gacccgtttg gggcgccaga gttttacctg gctgcgcgtc
     3721 tgtgcagatt cagagagcat ggaactcctg agactcacaa ctggagctta gaaggcgtaa
     3781 cgcacttcgc agccaggtaa ggtgcgtctt aacgcgtcga gaaaagattc gagatagcgg
     3841 cgcttctcgt ggactttgta gcgcaggttc cacatgccgt tttggctgag ctttttctgc
     3901 tggagctcag ggcgccacag ccagtcctcg gctcggtgat gccagcgcaa gttgacttcg
     3961 tgcagcgccg cattgtgcgt gaggaaaatg acttccgcct tgagctgctc tttcgcggca
     4021 gaggacagcg tcccagccaa tttcttgaag agctcgacgt aatcagcctc ccaac
//
LOCUS       DASUTG010000028         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_597173, whole genome shotgun sequence.
ACCESSION   DASUTG010000028 DASUTG010000000
VERSION     DASUTG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597173"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF089_00595"
     CDS             <1..223
                     /locus_tag="VF089_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172480.1"
                     /translation="LRMSNSKYMYEQQPEAPCHSRNMVLCASRGDKAGRIGTGAATPP
                     FATTHNQETIALPTSAVLNEIISASREGL"
     gene            220..1233
                     /locus_tag="VF089_00600"
     CDS             220..1233
                     /locus_tag="VF089_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172481.1"
                     /translation="MISSRGYRGFISAIVVLLIGFATSHAYSQESIRVAYSSTDTLNS
                     LWTIAAEAGFYKKHGLDAEVVYIGSTTVGVSAIVAQDVQIGNAAGSGVANAAVRGADT
                     VSVACFINVLPYELVVLDNIKSAEDLKGKSIGISRFGSVSDVAARELLKGLGLKPMED
                     VKILQVGGASERAAGFSRKIIAGFPSPPGNVHLIPGDLPHRVLANMADSEKPYPLPFI
                     CAVTTKSFLAKNRPIVKKAVMALIETGHFFKTNKEATQKIIAKYLRGANKAYLDSSYE
                     STAKLIERVPYTTREGMKIQLDDALKQNPGSKITVDNLIDDSIVREIEKEGFIDKVYG
                     KRG"
BASE COUNT          591 a          637 c          617 g          525 t
ORIGIN      
        1 attgcgaatg agtaattcca agtacatgta cgagcaacag ccggaggcgc catgtcactc
       61 ccggaatatg gttctatgcg ccagtcgcgg tgataaggct ggacggatcg gcacaggcgc
      121 agcaacaccg ccgtttgcca cgactcacaa ccaggaaaca attgcgcttc caacatcggc
      181 tgttctaaat gagataatta gcgcgtcaag ggagggctta tgatcagcag tcgcggttat
      241 cgtggattta tttctgccat cgtcgttctg ctaatcgggt ttgccacgtc acacgcgtat
      301 tcgcaggaaa gcatcagggt tgcgtacagc tccacggata cgctgaactc attgtggacc
      361 atcgccgccg aggctggatt ctacaaaaaa cacggattgg acgcggaggt cgtctacatc
      421 ggcagcacca ccgtcggtgt ctcagcgatc gtcgctcaag acgttcagat cggcaatgcc
      481 gcaggaagcg gggttgccaa tgcggcggta cgcggcgccg ataccgtaag cgtcgcctgc
      541 ttcatcaatg ttctgccata tgagctggtc gtcctcgaca acatcaaatc cgctgaagat
      601 ctcaaaggaa aatccatcgg tatcagccgc ttcggcagtg tgtctgacgt tgcggcccga
      661 gagctgctca aggggcttgg cttaaagcca atggaagacg taaaaatcct tcaggtcggc
      721 ggtgcctccg agagagccgc aggctttagc cgcaaaatca tcgccggatt tccctctcca
      781 ccgggaaacg tacatcttat ccccggtgac ctgccgcaca gagttttggc taacatggcg
      841 gattcggaga aaccgtaccc attgcctttt atctgcgccg taacgacaaa aagttttcta
      901 gccaagaacc gcccgattgt aaaaaaggct gtgatggcgt tgatcgaaac gggacatttt
      961 ttcaaaacca acaaggaggc cacgcaaaaa atcatcgcca aatatctccg cggcgctaac
     1021 aaagcctacc tcgacagttc gtatgaatcg accgcaaagc tcatcgagcg agttccttac
     1081 acgactcgcg aagggatgaa gatccagctc gacgatgctc taaagcaaaa tcccggcagt
     1141 aagataacgg tcgacaattt gatcgacgat agcattgtgc gcgaaatcga gaaggaagga
     1201 ttcatcgata aggtctacgg caaacgcggt tagcgactgg acgcagctaa ttggcccgcc
     1261 actttattga ttctgcggag cctcgttcgg actgcatgga acaatcgccg caccgcgagt
     1321 tcaagtagct gtgctagtaa ttctagtccg cgggatgtaa gccggtgtca agaggcggtg
     1381 gaacagagta actgacaatg gccgaatgga tcgaagtgcc ggcgcatcga atttatgtat
     1441 tatcagcgcg cgaatgcgag aatccttcga cgacatcggc gagaacggac gctccgcggc
     1501 gtgcgcgaaa gtgacttccc attttacgta agacgggcgg cacggttcta aaatgagcgt
     1561 gattcgtacc gagtcgaaat ggagtcgatg aactcacagt tcttaacggg atttaagtcg
     1621 gggacgatat gcgccactga gttataacaa aagcctgccg agccttgata gatgataacg
     1681 gcttggtact ctctctcact tcctcgccac gagtcagcac gatgtcgata cttatgctgc
     1741 cagcgatgat catgtgagct cttttcgcaa cagctctgct ctcgatatcc ctgcctggtc
     1801 ccctgacgca ctgcagaagt agcgcaaaat atcccatccg acatcgccag cgcacaaagt
     1861 tgtgacttct atctttctag accgagctgt cgatcctgaa aatccaattc actgagaatc
     1921 tgctgatccg caaactactg atattgctgc gcttacatgg aggagtctgg ctcgctgtcg
     1981 tggagtcgcc gggcacagcc attgctaaag ttttccccgg ccacaagttc tccgctcctg
     2041 agcaaggaag agcaagatgt ttaacttctt caatcaacgg cgcgcctggg acgatctgat
     2101 catcgtgttt cttaaatgcg gctgttcggg cgctggacta actatatcag cagcaatggc
     2161 gatgactctg gtagcgtgga gattattccg gagggaatct gctgaggaat ccagagatcg
     2221 ttcaatgccc agcactacta caaacgatct gccaccttgc tgccgtgcgc ccgaatgcgc
     2281 cttagagaga aacgccacgg cttcgcaaac tcccgcatcc ttagattagc ttcaaccatt
     2341 ggcaccgccc agattgcgcc tcttccgcct
//
LOCUS       DASUTG010000029         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1486913, whole genome shotgun sequence.
ACCESSION   DASUTG010000029 DASUTG010000000
VERSION     DASUTG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486913"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF089_00605"
     CDS             <1..854
                     /locus_tag="VF089_00605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6172482.1"
                     /translation="AAMRTNRIKLGTAIMTLTFREAVVLANQSATLNEISDGRAVLGL
                     GTGDGTTYTMARTAVPLEKFEEGTRVIRELVNGRTIHIPKGKEREAGEIPLRAGKFPV
                     PIWIAAAGPRALRAAGRVADGVILGCGFDLGVLTWAREQITLGALDVGRDPGEIEYVG
                     AGIACVDENGDRARDKARGRLANRAHQNFRFTLETVPPEERPGVEKFMKHFDVTKKID
                     QRSDPALVTDYLAQRFAIAGTPEECVARIKELEHAGIRSFLLTLPPKVYYDVMHRWAQ
                     TVVPLFS"
     gene            890..1684
                     /gene="pcaD"
                     /locus_tag="VF089_00610"
     CDS             890..1684
                     /gene="pcaD"
                     /locus_tag="VF089_00610"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX6172483.1"
                     /translation="MPFVQMGQIVHHFELKGSKDKSALVFSNSLGSDLRIWDAVADRL
                     SEHFSIVLYDKRGHGLSEASEPPYSASDLARDIVGLLDSLKIDQAVICGVSVGGIIAQ
                     AMALDHPRRVRALVLCDTGARIGSVESWQQRIDTVQSGGVQSLKTITMERWFSQGFRK
                     RFPADVRGYANMLLQTTVKGYIGTCCALRDADFRGDVSRLKQPTLVLCGAEDIATPPE
                     LGQELAGLISGSRFSSIANAAHLPCVEQPAAVVECMMQFFREVQIV"
     gene            1677..2063
                     /gene="pcaC"
                     /locus_tag="VF089_00615"
     CDS             1677..2063
                     /gene="pcaC"
                     /locus_tag="VF089_00615"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007673870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX6172484.1"
                     /translation="MSDELFDKGMAERKAVLGAEYVARAEANKTHFDADFQRYVTENA
                     WGAVWARPGLEKNIRSMLTIAMLAALGRHEELGAHIRATRNTGVTREEVKEVLLQVAV
                     YAGAPAANSAFAIAKRIYQEIEEEKK"
     gene            2124..2837
                     /gene="pcaH"
                     /locus_tag="VF089_00620"
     CDS             2124..2837
                     /gene="pcaH"
                     /locus_tag="VF089_00620"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX6172485.1"
                     /translation="MKIQPYKQRDWKSHPPYMFKAYGSSVKRGPLEKLVPITQTLSEI
                     TGPLFSDIKLEPGENDLTRSAGSGKEALGERIIVVGKVLDEDERPVPNTLIEIWQANA
                     AGRYHHPVDQHNAPLDPNFIGAGRCVTNDKGEYRYLTIKPGPYPWLNHPNAWRPSHIH
                     LSLFGPSFVTRLVTQFYFPGDPLLPLDPIFNSVPTKSARERLMASYAHDVTESQFALG
                     YRFDIVMRGRKATPFEESR"
     gene            2834..3406
                     /gene="pcaG"
                     /locus_tag="VF089_00625"
     CDS             2834..3406
                     /gene="pcaG"
                     /locus_tag="VF089_00625"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004865290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX6172486.1"
                     /translation="MRKETPSQTVGPFYAIGLTRKPMNHMVTEATQGQRIRIEGQVYD
                     GDGAAIPDVMVEIWQANAHGRYNHPDDKQEKPLDPTFTGWGRSGTDDKCFYDFETIKP
                     GPVPGAGDSVQAPHINVVVFARGTLVHAFTRIYFADEPANASDPVLNSIKNKKRRQTL
                     IASPGMENGKTVYRFDIRLQGENETVFFDM"
     gene            3511..>4581
                     /gene="pcaB"
                     /locus_tag="VF089_00630"
     CDS             3511..>4581
                     /gene="pcaB"
                     /locus_tag="VF089_00630"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX6172487.1"
                     /translation="MTMPLDSAIFAPLFTDEEVSRLLNDDAFVRALVEVEIALVRAEA
                     RVGVIPARAAEQIATTGAGKIDVGALAKGTVRSGFPIIALVQELRKLVGADAAPYVHW
                     GATTQDIMDTASVLQMRAAIQIFKRSIVEIVRRLSELAQRHRTTVMAGRTHGQQALPI
                     TFGLKVASWIAPMLRHVERIDEILSRLLVVQFGGAAGTLAALGDKGLAVTRALADELE
                     LSVPVMPWHAQRDSLVEFAGWLSLVTGSLGKMAQDVILLAQSEVAEVAESGEQGRGGS
                     STMPQKSNPITSELILAAARTNASLLSALHQAQIQEHERATHGWQVEWLTLPQMMIIT
                     GGALKHALDLAQNLQVDEAAMRV"
BASE COUNT          976 a         1258 c         1403 g          944 t
ORIGIN      
        1 ttgccgcgat gcgcaccaac cgaatcaagc tgggtacggc catcatgacc ctgacttttc
       61 gtgaggcggt ggtgcttgcc aatcagtcgg cgaccttgaa cgagatctcc gacggccgcg
      121 ctgtcctcgg cttgggcacc ggagacggga cgacctacac gatggcgcgc accgcggtgc
      181 cgctggagaa gtttgaagag ggcacacgcg tcattcgcga gctggtaaac ggccgcacca
      241 tccatattcc taagggtaag gaacgggaag ccggcgaaat tcccttgcgt gccggaaaat
      301 ttcccgtgcc gatctggatt gcggcagcgg gaccgcgcgc tctcagagcc gccgggcgtg
      361 tcgccgacgg tgtgatccta ggctgcggat tcgatctcgg agtcctgacg tgggctcgtg
      421 agcagatcac cctgggcgct cttgatgttg gacgagatcc tggggaaatc gagtatgtcg
      481 gcgccggaat cgcatgcgtg gatgagaatg gcgaccgcgc ccgggataag gcgcgcgggc
      541 gactggccaa ccgggcgcat caaaattttc gctttacgct ggaaacggtt ccgcccgaag
      601 agcggccggg tgtagaaaaa ttcatgaagc atttcgatgt caccaaaaaa atcgatcaac
      661 gttccgatcc tgctctggtc actgattatt tagcgcaaag atttgcgata gctggcactc
      721 cggaagagtg cgtcgcgcgg attaaagagc tcgagcacgc cggcattcgt agttttttgt
      781 taaccttgcc gccgaaggtt tactatgacg ttatgcatcg atgggctcag acggttgtgc
      841 cgctattctc ctaaagtgag ttcggttgga ctggaggcgt gaacgctcga tgccttttgt
      901 gcagatgggt cagattgttc atcacttcga gctcaagggg agcaaggaca agtctgcgct
      961 ggtgttttcg aactcccttg gcagcgattt gcgtatctgg gatgctgtag ccgaccggtt
     1021 gtccgagcac tttagcatcg tcctctacga taagagaggg catggtctct cagaggcttc
     1081 ggagccgccg tattctgcga gtgatctggc tcgggatatc gtcggattgc tggattcatt
     1141 gaaaatcgat caggcggtga tttgcggagt ttcggtgggt ggcatcatcg cgcaagccat
     1201 ggcattggat catccgcgcc gggtgcgtgc tctggtgctg tgcgataccg gggcgcgaat
     1261 aggctcggtg gaatcatggc agcagcgcat cgatacggtt cagagcggag gtgtccaatc
     1321 tcttaagacc ataaccatgg aacgctggtt ctcgcaggga tttcgcaagc gcttccccgc
     1381 cgatgtgcgc ggctatgcca acatgttgct tcaaaccacg gtcaagggct acataggtac
     1441 ttgctgcgcg ctgcgcgacg ccgattttcg cggcgacgtg tcccggctga agcaaccgac
     1501 attggtgctg tgtggcgcgg aagatatcgc gaccccaccg gagcttggac aggaactggc
     1561 tggtttgatt tccggcagcc ggttctcttc cattgccaac gcggctcatc tgccgtgcgt
     1621 cgaacagccg gcggcggttg tcgaatgcat gatgcaattt tttcgggagg tgcaaattgt
     1681 ctgatgagct atttgataaa ggcatggcag agcgcaaagc ggtgctcggt gcggaatatg
     1741 tcgcccgggc ggaagccaat aagacccatt ttgacgcaga cttccaacgc tacgttaccg
     1801 agaacgcctg gggcgcggtt tgggcccgac cggggctcga aaagaatatc cgcagtatgc
     1861 tcaccatcgc catgttggcg gcgctcggcc gtcatgaaga attgggcgcc catatccgcg
     1921 cgacgcgcaa taccggcgta actcgggaag aggtcaagga agttctgttg caagtggcgg
     1981 tctatgccgg cgcgccggcg gcgaacagtg cctttgctat tgccaaacga atttaccagg
     2041 agattgaaga agaaaaaaaa tagcaaaagg gcgatagcaa tggagaaact gattctcgac
     2101 ggcggtgttt ggagattgtg aaaatgaaaa tccaacccta caaacagcgt gattggaaat
     2161 cccatccgcc gtacatgttc aaggcgtacg gctcctcggt caaacgcgga cctctggaaa
     2221 agctggtgcc gatcacgcag accttgtctg aaatcaccgg gccgctcttc agcgatatca
     2281 agcttgagcc cggcgagaac gatcttacgc gcagcgctgg gagcggcaag gaggcgctgg
     2341 gtgagcggat cattgttgtc ggcaaggtct tggatgaaga cgagcggccg gtgcccaata
     2401 cgttaatcga aatctggcag gccaacgccg cgggccgcta tcaccacccg gtggatcagc
     2461 acaacgctcc actggacccg aactttatcg gcgccggacg ctgcgtgaca aatgacaagg
     2521 gggaatatcg ctatctcacc atcaaacccg gtccttatcc atggctgaat catccaaatg
     2581 cctggcggcc ctctcacatt catttatctt tgtttggacc gagcttcgtt acccgcttgg
     2641 tgacgcagtt ttattttccc ggcgacccgc ttttaccgct cgatccgatt ttcaattccg
     2701 ttccgaccaa gagcgcacgc gaacggctga tggcgagtta cgctcatgac gtgaccgagt
     2761 cccagttcgc gctcggctac cgcttcgaca tcgttatgcg cggccgcaaa gccacgccct
     2821 tcgaggaatc gcgatgagaa aagaaacgcc ctcccagacc gtcgggccgt tttacgccat
     2881 cggcctgacg cgcaagccga tgaaccatat ggtcactgaa gcgacgcaag gccagcggat
     2941 ccgcatcgaa ggacaagtgt acgacggcga cggcgcggcg attcccgatg tcatggtaga
     3001 gatctggcaa gccaacgctc acgggcgcta caatcatccc gacgacaagc aagaaaaacc
     3061 gctcgatccg acgtttaccg gctggggtcg ttcgggcacg gacgataagt gtttttatga
     3121 tttcgagacg atcaagcccg ggcctgtccc gggagccggc gacagcgtgc aagcgccgca
     3181 catcaacgtc gtggtcttcg cccgcggcac gttggtgcat gcatttacgc ggatctattt
     3241 tgctgacgag ccggccaacg ccagcgaccc ggtgctgaat tcgatcaaga acaagaaacg
     3301 ccgccaaaca ttgatcgcgt cgcccggaat ggaaaacggc aaaaccgttt accgtttcga
     3361 tattcgcctg caaggcgaaa acgagacggt tttctttgat atgtgaggag agaaacaagt
     3421 agatgctgcg ctgttctcgg atgacagtag ttttccgtca tcctgaacgc agtgaaggat
     3481 ctgctttaag ggtcctccgc gataatttca atgaccatgc cgctcgattc cgcgatcttc
     3541 gcgccactgt ttaccgacga agaagtatcg cgcctgctca atgacgatgc cttcgtgcgc
     3601 gcgctggtcg aggtcgagat cgcgctggtg cgggccgagg cgcgagtcgg cgtgattccg
     3661 gcgcgcgcgg cggagcagat cgccacgacg ggagccggga aaatcgacgt tggcgctctg
     3721 gccaaaggga ccgtccgctc cggttttcca atcatcgccc tggtgcaaga gctgcgcaag
     3781 ctggtcgggg ccgatgccgc gccctacgtg cactggggtg cgacgacgca ggacattatg
     3841 gacacggcgt ctgtgctgca aatgcgcgcg gcgatccaaa ttttcaaacg ctcgattgtc
     3901 gaaatcgtcc ggcgcttgag cgaactggcg cagcggcatc gtactaccgt gatggctggg
     3961 cgtactcatg ggcagcaggc gctgccgatc actttcgggc tgaaagtcgc cagctggatt
     4021 gcgccaatgc ttcgccatgt tgaacgcata gacgagatct tatcgcgcct gcttgtcgtc
     4081 caattcggcg gcgccgcagg caccctggcc gctttggggg acaaaggtct agccgttacc
     4141 cgagcattgg cagacgagct cgagttatcc gtgccggtaa tgccgtggca tgctcagcgc
     4201 gacagcttgg tagaattcgc cggttggctg agcctggtga cgggatcgct cgggaaaatg
     4261 gcgcaggatg tcattttgct ggcgcaaagc gaagtcgccg aagtagccga atccggcgag
     4321 cagggcaggg gtggcagcag taccatgccg caaaaaagca atccgatcac cagcgagctc
     4381 atactcgccg cggcgcgcac caatgcttcc ttactatccg cgctgcatca ggcgcagatc
     4441 caagagcacg agcgggcgac gcatggctgg caagtcgaat ggctgacttt gccgcaaatg
     4501 atgattatca ccggcggcgc gctcaagcat gccctcgatc tagcgcagaa tctgcaagtg
     4561 gatgaggcgg cgatgcgggt t
//
LOCUS       DASUTG010000030         4473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1486994, whole genome shotgun sequence.
ACCESSION   DASUTG010000030 DASUTG010000000
VERSION     DASUTG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486994"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VF089_00635"
     CDS             <1..1038
                     /locus_tag="VF089_00635"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172488.1"
                     /translation="LKGIDADLNYGLKKLVGASQWKSITKRKAAPVGLACAVTNAGAT
                     PVSVAMARLQSDGVVNLFAGSTEMGQGVRTVLSQIAAEELSIPIDLVRVAGADTKVTP
                     YDSSTGSSRSTTCMGNAVQGAARDLRKQLVTIAAETFRVPQNQVKLQNGSLISGEARL
                     TFQEALERRFGKGSGGELIGRGETTPGLTENKMPVFWEVGMGMVELQIDQDTGEIGVK
                     RLISVADVGKAIHPQGCVGQEEGAAMMGIGHTLFEQMVYDTNGNLLNPSLVDYRVPSF
                     NDTPEEFRTVLVENRDGPGPYGVRGMGEGGLLSVAPSVCNAFARATGVRIKDLPLTPE
                     RVWRALKEAKK"
     gene            1092..1580
                     /locus_tag="VF089_00640"
     CDS             1092..1580
                     /locus_tag="VF089_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6172489.1"
                     /translation="MSLPDFEIRPAAIADVPLILSFIKKLAEYERLAHEVVATEESLR
                     ETLFNARKSAEIAIGYYQNKPIGLVLFFLNYSTFLGRPGLYIEDLFIDESYRRRGFGA
                     ALLRYVARLAIERNCGRLEWSVLDWNKPAVDFYTKLGAVPMSGWTVFRVTGESLANLA
                     AE"
     gene            complement(1638..2294)
                     /locus_tag="VF089_00645"
     CDS             complement(1638..2294)
                     /locus_tag="VF089_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6172490.1"
                     /translation="MRNTRPERKAVADALVRERQRISRELHDRALQILSSVRLRAEVC
                     RDQLVTDQEPLRRELKIIEKEIDTVILEIRNILTETQEQKELKAGSLERRLKAELEIF
                     RARTGFNLDFRCTIHARDLPQEIERELYFTLREGVLNAVRHARASQLTLALSRDATSC
                     NAQLIDNGVGFDMSTAEGGNHYGLRSMRERIKRVGGELTIESAPGTGTRLLIRIPLSE
                     "
     gene            complement(2336..3049)
                     /locus_tag="VF089_00650"
     CDS             complement(2336..3049)
                     /locus_tag="VF089_00650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6172491.1"
                     /translation="MAQSSWQQTTSDSSTMRLHLSVVEGVGPFPGIVVIQHQWGLDEF
                     MQSITARLAQAGYVAAAPDLYHRDGPECTDDVIARRARLSDRRVINDVNASVGFLRSQ
                     PAVDRNCLGIIGFCMGGRIAYLMAAASPEFRAGVVFYGGNIFRAWGRDIPSPFERTSE
                     IHCPLQGHFGADDKNPSLDDMRKLDLELTNFDKRHEFYSYPDAGHAFMDHTRESYRRH
                     ADEAAWPRTLDFLSRHLST"
     gene            complement(3117..3821)
                     /locus_tag="VF089_00655"
     CDS             complement(3117..3821)
                     /locus_tag="VF089_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6172492.1"
                     /translation="MPIKAVFFDAAGTLITPIRPVGQTYTLLAKNYGVEVSSFEISER
                     FRTCFAASPPLTFGPALTEPIERLEREWWKKLVQCVFQPLERFEQFDRFFTELFSYFA
                     RPQAWTLYPDVRETLSALQERGLALDVISNFDSRLIEILEGLGIAGCFEEVFISSRVG
                     HAKPERQIFETALRRHNLAPGEAMHVGDSQEHDLYGAANAGLLGVLIDRSASGWPGDY
                     RQIKSLNEIIPILNHV"
     gene            3891..>4473
                     /gene="pncA"
                     /locus_tag="VF089_00660"
     CDS             3891..>4473
                     /gene="pncA"
                     /locus_tag="VF089_00660"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX6172493.1"
                     /translation="MEINKALLIVDVQNDFCPGGALGVQDGDRVVPVLNWYIEEFARA
                     GMPIFVTRDWHPPRTSHFNTAGGVWPPHCVQGSKGAEFHPDLKISNEAIVVSKGTAVD
                     EDSYSAFAAADARGVALKDLLRQRGIERIYVGGLATDYCVKETVLEGLMEGFKVVLLQ
                     DAVCGVNLQPEDSAQAIDAMVKAGVTVAPPVDSF"
BASE COUNT          994 a         1167 c         1283 g         1029 t
ORIGIN      
        1 cttaaaggca tcgatgccga tttgaattac ggccttaaga agctcgtcgg cgcgagccaa
       61 tggaaatcca taactaagag gaaggccgcg ccggtaggat tagcctgcgc cgtgaccaat
      121 gcgggagcaa cccccgtatc ggtcgcgatg gcacgcctgc agtccgacgg cgtggtgaat
      181 cttttcgccg gcagcacgga gatgggacaa ggagttcgga cggttctctc gcagatagcg
      241 gccgaggaat tgagtatccc gatcgatctt gttcgagtgg caggcgccga caccaaagtc
      301 acaccttacg atagctcgac gggttcgagc cgttcgacca cctgtatggg gaacgcggtg
      361 caaggcgccg cccgggattt gcgaaaacaa ctggttacga ttgccgcgga gacatttcgc
      421 gtcccacaga accaagtcaa actccagaat ggctctctga taagcggtga ggcgcgcctg
      481 acattccaag aagcgctcga gcggcgtttc ggcaaaggct caggcggcga gttaatcgga
      541 cgcggcgaga ccactccggg attgacggag aacaaaatgc cggtgttctg ggaagtcggc
      601 atgggcatgg tcgagctcca gatcgaccaa gataccggcg aaatcggcgt caagcgcttg
      661 atctccgttg ctgatgtcgg caaggcgatc catccgcagg gctgtgtggg ccaggaagag
      721 ggcgcggcca tgatgggcat cggtcataca ctgtttgaac agatggtcta tgacaccaac
      781 ggtaatctgc tcaacccgag tcttgtcgat taccgggtgc cgagtttcaa cgatactcct
      841 gaggagttcc gcacggtgtt ggttgagaac cgggatggcc ccggtcccta cggagtccgt
      901 ggtatggggg aaggcggtct gctatcggtc gcgccttcgg tttgtaatgc tttcgcgcgg
      961 gccacagggg ttcgtatcaa agacctgccg ctaactcccg agcgtgtctg gcgcgcgctt
     1021 aaagaagcga agaagtaacc ttcctgcttg atcgcggaaa tcagcgtaat gatttactgc
     1081 taaagagaca gatgagttta cccgactttg aaatacgacc tgccgcgatc gctgacgtgc
     1141 cgttgattct ttccttcatc aagaagctcg ccgagtatga aaggcttgcg cacgaagtcg
     1201 tcgcgacgga ggagtcgctg cgggagacgc tgtttaacgc tcggaaatcg gctgaaattg
     1261 ccatcggcta ttaccagaac aaaccgatcg gcttggtgct gttcttcctc aactattcga
     1321 cttttttggg ccggccggga ctctacattg aggatctctt catcgatgaa tcgtatcgca
     1381 gaagggggtt cggtgcggca ctactgcgct acgtagcgcg cttagccatc gaacgaaact
     1441 gcggccgcct cgaatggtct gtacttgact ggaataaacc cgctgtagac ttctatacca
     1501 agcttggtgc ggtcccaatg agcggttgga ccgtgtttcg ggtcaccgga gaaagtttgg
     1561 cgaatctcgc ggcggaatga atctccgcgc agcatcacag gttcgctata aagacctggt
     1621 gagaatcgtg atgcttctta ttcgctcaag ggtattctga tcaagagccg tgtgccagtc
     1681 ccaggcgccg actctatcgt gagctcgccg ccaaccctct tgattcgctc ccgcatgctc
     1741 cttaggccgt aatgattgcc gccttccgcc gtcgacatat cgaaccccac cccattatcg
     1801 atcaattggg cgttgcagct cgtagcatct ctggagagcg ccaatgtcag ttgcgatgca
     1861 cgagcatgtc gcacggcgtt cagaacgcct tctctcaatg tgaagtacaa ttcccgttcg
     1921 atctcctggg gcaagtcacg cgcatggatg gtgcagcgaa aatccaagtt gaaaccggtg
     1981 cgcgcgcgaa agatctccag ttctgccttg agtcttcgct ccaacgagcc cgccttgagt
     2041 tctttttgct cttgggtctc ggtgagtatg ttgcgtattt cgaggatgac tgtgtcgatt
     2101 tctttctcaa tgatctttag ttcccgccgt aacggctctt ggtccgtgac gagctgatcg
     2161 cgacatactt cggcccttaa tctgacgctt gacaggattt gcagcgcccg gtcatggagc
     2221 tcccgggaga ttcgttggcg ctctcgaacc agggcgtccg ccacggcttt gcgttccggt
     2281 ctggtattcc gcacgttgtt gtccttgatt gactatctgt tcatggtgca accggctaag
     2341 ttgacaaatg cctggagaga aagtccaaag ttcgtggcca agcagcttca tctgcatggc
     2401 ggcgatagct ttcacgggtg tggtccataa atgcatggcc ggcatccgga tatgaataga
     2461 actcgtgcct cttgtcaaaa ttcgtaagct caagatcgag cttgcgcata tcatcgagag
     2521 aagggttctt atcgtcggcg ccgaagtgac cctggagcgg acaatgaatt tccgaagtac
     2581 gctcaaatgg cgagggaata tcgcggcccc aggcgcgaaa gatgttacct ccgtaaaaaa
     2641 cgacgcctgc tctaaactcg gggctggcag cagccatgag gtaggcgatc cgaccaccca
     2701 tgcaaaaacc gattatgccc aaacaattcc gatcaaccgc aggttgagag cgcaggaatc
     2761 ccacgctcgc attgacatca ttgatcaccc tgcgatcact gagccgggcg cggcgcgcaa
     2821 tgacatcgtc ggtgcattcg gggccatcgc ggtgatagag atccggcgca gcggcgacgt
     2881 agccggcttg cgctaagcga gctgtgatgc tctgcatgaa ttcgtcgagg ccccactggt
     2941 gttgaataac tacgatgcct ggaaacggac cgactccttc gacgacgctc aagtgcaggc
     3001 gcatcgtgct actgtcggat gtagtttgtt gccagctgga ttgtgccatt tatttccctt
     3061 aaagttagca ggcgtttacc gtaggacccc ttagcgtgtc aagtcttgaa ccgcctttaa
     3121 acgtgattga gaattgggat gatttcgttg agacttttaa tctggcgata gtctcccggc
     3181 catccagacg cgctgcgatc gatgagaact cccaacagtc cggcattggc ggcgccgtac
     3241 agatcgtgtt cttggctatc gccgacgtgc atggcttcgc cgggcgctag gttgtggcgt
     3301 cttagggccg tttcaaagat ctgccgttca ggtttggcat gaccgacccg gcttgaaata
     3361 aaaacctctt cgaagcagcc ggcgatcccg agcccctcga gtatctcgat tagccgagaa
     3421 tcaaaattgg agatgacatc caacgccaag ccgcgttctt gcaaggccga caaggtttcc
     3481 cggacatcag gatataacgt ccaagcctgc ggtcgcgcaa aatatgaaaa cagctcggta
     3541 aaaaaccggt caaattgctc aaagcgctcc aacggctgaa agacgcactg cactagtttt
     3601 ttccaccact cgcgctcgag ccgttcaatt ggttccgtca aagccggacc aaacgtgagc
     3661 ggaggcgagg cggcgaaaca ggtgcgaaag cgttcggaga tctcaaaaga agaaacctcc
     3721 acaccgtagt ttttcgcgag caacgtataa gtttgaccca ccggcctgat gggcgtaatg
     3781 agggttcccg cggcgtcaaa gaaaactgct ttgattggca tcagagatcc atttgttgcg
     3841 atcgtctcaa ttgcaacgac cgtatcattc tctataatga atacaaatcg atggaaatta
     3901 acaaagcgtt attgattgtt gacgtgcaaa atgatttctg tccaggcggt gctcttggcg
     3961 tgcaggacgg cgatcgtgtt gtgccggttc ttaattggta tattgaggag tttgctcgag
     4021 cgggcatgcc gatctttgtt actcgggatt ggcatccgcc gaggacgagt cacttcaata
     4081 cggcgggggg ggtgtggccg cctcactgcg tccaaggatc aaaaggcgcg gagtttcatc
     4141 ctgacttgaa gatcagcaat gaggctatcg ttgtttcgaa ggggaccgct gtagacgagg
     4201 atagctactc ggcttttgct gcggcggatg cgcgcggcgt ggcgcttaaa gatcttttac
     4261 ggcagcgagg aatcgaacgg atttatgtcg gtgggttggc cactgattat tgcgttaaag
     4321 aaactgtgct ggaaggcctc atggaaggat ttaaggttgt gctgcttcaa gacgcagttt
     4381 gcggcgtcaa tctacaaccg gaagattctg cgcaagccat tgacgccatg gtcaaagccg
     4441 gggttacggt cgcacctccc gtcgacagtt ttc
//
LOCUS       DASUTG010000031         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1042520, whole genome shotgun sequence.
ACCESSION   DASUTG010000031 DASUTG010000000
VERSION     DASUTG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042520"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VF089_00665"
     CDS             complement(<1..586)
                     /locus_tag="VF089_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX6172494.1"
                     /translation="MVKVKVCGITSLADAEKALEYGADALGFNFYAPSPRYLAPEKAR
                     AIVQQLPAGAWNVALFVNEAKERIRQVLAAGTMPDGRSGFKSIQFHGEESPNDCRGWD
                     IQVIKAFRLKDKQSLEKLHNFPADYYLLDSWSAGYGGSGASFPWEWLEGFDTEKIILS
                     GGLNLDNVAEAIRRLHPYAVDVCSGVELRPGVKDH"
     gene            complement(643..1422)
                     /gene="trpC"
                     /locus_tag="VF089_00670"
     CDS             complement(643..1422)
                     /gene="trpC"
                     /locus_tag="VF089_00670"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX6172495.1"
                     /translation="MADFLADIVEHVRTVVEQRQKQAPLASLHDRPLYHQPTRGFARG
                     LSGGTRRIIGEIKRSSPSKGLIRADFSPVIIARDYADHGASAISVLTEEHFFQGSLTY
                     LEEIKAAVPVPLLRKDFIIDPYQVTEARSYGADAILLIAALLDPALLRELRETAAALS
                     LDTLVEVHNEEELACALEAGAEIIGINNRDLKTFEVSLATTEKLAPRVPAGTLTICES
                     GIDGAEQIRQVERWGIHTFLIGESLMRAPSPGEKLQELLRS"
     gene            complement(1429..2442)
                     /gene="trpD"
                     /locus_tag="VF089_00675"
     CDS             complement(1429..2442)
                     /gene="trpD"
                     /locus_tag="VF089_00675"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6172496.1"
                     /translation="MNIREAIEKLVKRDNLSEEDTIDVINQIMTGEATPLQVASFLTA
                     LRMKGETVEEITGAARVMREKAHRVQVGSKVVLDTCGTGGDQKGTFNISTAAAFVAAG
                     AGVDVAKHGNRSVSSQSGSADVLAALGVKVDAPRERVESCIRQIGIGFLFAPLLHEAM
                     KYAVQPRRDIGIRTVFNLLGPLTNPAMANHQLIGVYDGELVGVIAHVLKNLGSARAMV
                     VHGMEGLDEISLCGPTKIAELRHGQVKEYTVEPERIGFKRYPLEALHAGSATESATII
                     KRIFQGEKGPARDVVLLNSGAALYVSGKAETLQDAIRVAADSIDSGRARQKLEQLAQL
                     TNA"
BASE COUNT          548 a          733 c          657 g          570 t
ORIGIN      
        1 catgatcctt aacaccgggt cgcagctcta cgccactgca cacgtcgacg gcatacggat
       61 gaagccgccg aatagcctcg gcgacattgt ccagattgag cccgccggag agaataattt
      121 tttcagtatc aaatccttcc agccattccc acggaaatga cgcgccggag ccgccgtatc
      181 cagcggacca agagtcgaga aggtaataat ccgcaggaaa gttgtgaagc ttctccaaag
      241 attgtttatc tttgagccga aaagccttga tcacttgtat gtcccagcca cggcagtcat
      301 tgggactctc ctcgccatga aattgaatac ttttaaaccc ggatcgcccg tcaggcatag
      361 tgcccgcagc taaaacttgc ctgatccgct ctttagcctc attgacgaac aaggcgacat
      421 tccatgcgcc cgcaggcaat tgctgcacaa tggcacgagc cttctccggg gcaagatagc
      481 gcgggcttgg cgcatagaaa ttaaagccaa gcgcgtcggc gccatattcc aatgccttct
      541 cggcatccgc caagcttgta atgccgcaaa cttttacctt aaccatgatg atgggccaac
      601 gtcataagca aatagtagat tgcaggctgc ttcttaggaa atctaggatc gcagcaactc
      661 ttgaagcttc tcacccggac tgggcgcgcg catgagagat tcgccgatga ggaaggtatg
      721 gatgccccac cgttcaactt gccttatttg ttcagcgccg tcgataccac tctcacagat
      781 tgtgagagta ccggcgggaa cgcgcggtgc caacttctcc gtagtcgcca gactgacttc
      841 aaaggtcttg agatcccggt tgttgatgcc gattatctcc gcgccggcct caagcgcgca
      901 cgccaactct tcctcattgt gaacttcgac cagggtgtcg agcgataaag cagccgccgt
      961 ttcacggagc tcgcgcagaa gcgctggatc cagcaatgcg gcgatcagca agattgcgtc
     1021 ggcgccataa cttcgtgcct cggtcacctg gtaaggatca atgataaaat cttttcgcaa
     1081 cagcggcacg ggaaccgcgg ctttgatctc ttccaggtat gtcaaactac cctgaaaaaa
     1141 atgctcctcg gtgagcaccg aaatcgcgct ggcgccatga tcggcataat ctctcgcaat
     1201 aatcaccgga ctgaaatcgg cccgaatcaa gcctttcgag ggcgacgagc gcttgatttc
     1261 gccaataatg cgccgggtcc cgccactcag gccgcgggca aagcccctgg tcggttgatg
     1321 atacaaaggc cggtcatgca atgacgccag gggggcttgc ttttgccgct gttcaactac
     1381 tgtgcgaaca tgctcgacaa tgtcggcgag aaagtcagcc atgggcggtt acgcgttggt
     1441 cagttgcgcc aattgctcga gcttttgccg cgcccttccg gaatcgatgg aatctgccgc
     1501 cacccggatt gcgtcttgga gtgtctcggc tttgccgctg acatacaatg ctgcgccgct
     1561 gttgagcagc accacgtcgc gcgccggtcc cttctcacct tgaaagatgc gcttgatgat
     1621 cgtggcgctt tcggtggcgc tgccggcgtg gagagcctct agaggatatc gcttgaagcc
     1681 gatcctctcc ggttcgaccg tgtactcttt gacttggccg tggcgcagct cggcgatttt
     1741 tgtcgggccg cagagtgata tctcatccaa gccttccatg ccgtgaacca ccatggcgcg
     1801 cgcgctgccg agatttttta acacgtgtgc gatcacccct acgagctcgc cgtcgtaaac
     1861 gccaatcaat tggtggtttg ccatagcagg attggttagc gggccgagca aattgaagac
     1921 ggtccgtata ccgatatcgc ggcgcggttg cacggcatat ttcatcgcct cgtgcagtaa
     1981 tggagcgaac aaaaatccaa tgccaatctg tcgaatacag ctctcaaccc gctctctggg
     2041 cgcatcgact ttaactccca aagcggcaag cacatcggcg cttcccgact gactcgacac
     2101 tgaacgattg ccatgtttgg cgacatcgac accggcgccg gcggcgacaa aggccgcagc
     2161 tgtcgatatg ttgaaagtgc ctttttgatc cccgcccgtg ccacaggtgt ccaacacgac
     2221 tttggagccg acttggacac gatgagcctt ttcgcgcatc acccgcgccg cgccggtgat
     2281 ttcttctacg gtttcgcctt tcatacggag cgccgttaga aaggatgcca cctgcaaagg
     2341 cgtcgcctca ccggtcatga tttggttgat gacatcgatg gtgtcttctt cggaaaggtt
     2401 atcgcgcttg accaatttct cgatcgcttc tcgaatattc atagtcgcct ggatctctag
     2461 caggctgctg aaaaagaccc atatgcttcg ttgccgctca atcgcctc
//
LOCUS       DASUTG010000032         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2080293, whole genome shotgun sequence.
ACCESSION   DASUTG010000032 DASUTG010000000
VERSION     DASUTG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080293"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF089_00680"
     CDS             <1..200
                     /locus_tag="VF089_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX6172497.1"
                     /translation="YFGYNGNLDTCIVIRTIVMKGSKVFITAGAGIVADSDPDAEYQE
                     TLSKARAMFRAVELAEQWSKP"
     gene            197..763
                     /locus_tag="VF089_00685"
     CDS             197..763
                     /locus_tag="VF089_00685"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX6172498.1"
                     /translation="MILLIDNYDSFTYNLYHYLGELGANVKVYLNDTISLDEIAELRP
                     EKIVISPGPCTPKEAGISCAVIRRFSGQIPTLGVCLGHQAIGAAFGGEIVRAPTIMHG
                     KLSEVAHDSQTIFRSIKNPFAAMRYHSLVIERQSLPDDLIVSAQTADGVIMAVRHKSM
                     PVEGVQFHPESILAEDGKKVLQNFLDYY"
     gene            780..1199
                     /locus_tag="VF089_00690"
     CDS             780..1199
                     /locus_tag="VF089_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HEX6172499.1"
                     /translation="MRLLCFQAKHFRWKTHSKTLPEVPHLDAEDEIADTVVIFVQAEA
                     SDTAERHRDSIRRKAVKHIKWLANKRDLKNVVLHSFTHLGGETAEPGFAEPFIQSIAD
                     RLNQSGYRVQITPFGYFCEWDLSVYGESLAKVWKEIH"
     gene            1288..1836
                     /locus_tag="VF089_00695"
     CDS             1288..1836
                     /locus_tag="VF089_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6172500.1"
                     /translation="MKLIRSKIFSLTFLMICLTVFGCRKGEELAAPDDLPDISKQIRP
                     YVTVASTKVRTGPGPQFRATGEVPPRAKVHVVGRDGDWVLIVSKKGNIPGFIEMASVK
                     PGEGEESATGDPAVGGKYEILANTKLYSGPGLHYPVVAEVSKGTIINVVEEDKGWIKV
                     ESKRGKQPGYVESSLAKPAADQ"
     gene            2194..2658
                     /locus_tag="VF089_00700"
     CDS             2194..2658
                     /locus_tag="VF089_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6172501.1"
                     /translation="MEIERKLAELGIEVPAPPVPAAHYIPYVLVGNLLFVGGNTGRLN
                     GQPHLYAGKVGDHVTLEQAYQMARHCALNHLAIIKSAVGDLDRVERFVKLLGFVNVAP
                     GFTEMPQVVNGASDLFIEIWGERGQHARAAVGVASLANEAPVETEVIVQIRD"
     gene            complement(2964..5252)
                     /locus_tag="VF089_00705"
     CDS             complement(2964..5252)
                     /locus_tag="VF089_00705"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6172502.1"
                     /translation="MAKPELKIIGSSPARMGGIERVVGKGVYGIDLMFKDQLHGAILR
                     SQYAHAKIVSIDTSEARKIPGVHAVVTAEDAPNVRYGRSYIDRHILARGKVRFMGDPV
                     AAVAAESSAIARQALKKIKVVYEPLPVVIDPEEAIKADAPTLHDDMPLPKTIPADAGL
                     KNICGHTVVHIGDAEKAMAEADLVVDEVYETKMIHPQYLEPRIAAAQMEPNGRLTVWA
                     NAQAPFPVRQEVAKTLGLPLNNVRVISAGLGGGFGGKGSGITSGAAVEPICGLLAIKA
                     KRPVMIVLDKAEETISTTIRSGARMYIKTGVKKDGTIVARTGKVFYDAGGYSGFGVQA
                     GGRCTNMLGGWYLMPNCHIEGYSVYTNKQVCGPVRGPGGPQAAFAVESHMDSIAAKLG
                     MDPVEFRLKNVPKAGDKIVGVPKLRDVSLGETIRIAKEKIGWGNVKLEKNQGIGLATG
                     SWIESAGPGGGAIVKVNEDGSVTVHIGKIDMGTIPGFGIPMIVAEELGIPVSDVTVVN
                     VDTDASPWDAGTVGSRGILVSGTASRLAAIDARNQLFKMAARQLEASPDDLEIVDKQI
                     RVKGTPAKAIALATVATNAHNVIGEVIGRGYCDNLELMAEEKAHGSSQPFTAHACIVE
                     VSPDTGNVKILKYVAVHDIGQPIHQAAVEGQIEGATAMSIGQALCEQVVFDKDGRTLN
                     PSFVDYLMPTINMMPRIETTLVPGYPGAGPYGAKGAGEIACVPPMACIANAIFNATGV
                     RINKLPLSPENVLRGLKAAGKA"
     gene            complement(5256..5732)
                     /locus_tag="VF089_00710"
     CDS             complement(5256..5732)
                     /locus_tag="VF089_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6172503.1"
                     /translation="MSVTINTKINGQAVSSSADASTSLLEFLRDTLEMKGSKLCCNTG
                     ECGACTVIYNGKPINSCVTMAADANGAEIITIEGLANGDKLHPVQQAFIDTGAVQCGY
                     CTPGYIISVKALLDRTTKPTAEDIEEAVSGNICRCTGYNKIVDAIHLAADRIAGRA"
     gene            complement(5733..6602)
                     /locus_tag="VF089_00715"
     CDS             complement(5733..6602)
                     /locus_tag="VF089_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6172504.1"
                     /translation="MEYYQPKTVNEAVSLLAKHGGEAKIIAGGTDVMVDIKYKEEPAC
                     LINIKKLPGLSGIQENGGSLRVGPLVTIRELETNTILRDKLPVLWEAAHQFASLQVRN
                     TATIGGNICRASPSGETLAPLLVLEASAKCAFSEGEQNQPFMKFFQGPGKSVLGGKGL
                     LTEIDVPYPPAGSKGVYLKHAVRGAMDIAMVGVAVLITPDAAKSNIQDVRIGLGAVAP
                     TPVRAPKTEALLRGKPLTAALLKEAGALGATEASPIDDQRSSAENRRWIVEALTRRGL
                     EQTWKAVTGKEVA"
BASE COUNT         1362 a         1935 c         1874 g         1509 t
ORIGIN      
        1 tatacttcgg ctacaatggc aacctcgata cctgcatcgt cattcggacg attgtgatga
       61 aaggcagcaa ggtattcatc accgccggag ctggaattgt cgccgactcg gatcccgacg
      121 cggagtatca ggagacgctg agtaaggcgc gggcgatgtt tcgcgctgtg gagctcgccg
      181 aacagtggag caagccatga ttctgctcat cgacaattac gattcgttta cttacaacct
      241 ttatcactat ctgggcgagc tcggggcgaa tgtaaaggtt tatctgaacg acacgatcag
      301 tttggacgaa atagccgagc taaggccgga gaagatcgtt atctctccgg gcccctgcac
      361 gcctaaagag gcagggatct cctgtgcagt aatccggcgt ttcagcggcc aaatcccaac
      421 actcggcgtc tgcctgggcc accaggcgat cggagcggct tttggcggcg agattgtccg
      481 agcgccgacg atcatgcatg gcaagctctc ggaagtagct catgattcgc aaacaatttt
      541 ccgcagcatc aaaaacccct ttgccgcaat gcgttatcac tcgctggtga tcgagcgaca
      601 gagcctgccg gacgatctga tcgtcagcgc gcaaactgca gacggcgtca tcatggccgt
      661 acgccataaa agtatgccgg tcgaaggcgt gcagtttcat cccgaatcga tacttgccga
      721 ggacggcaag aaagtcctac aaaacttttt agactattac tgataacgat aagcctttca
      781 tgagacttct gtgtttccaa gccaaacact ttcgttggaa aactcattct aaaaccctcc
      841 ccgaagtccc gcatctggat gcggaggatg aaattgccga cactgtagta attttcgtcc
      901 aagccgaagc gagcgatacc gcagagcgac atcgcgactc gattcggcgc aaagcagtca
      961 agcatattaa atggctggcg aataaacggg atctcaagaa tgttgttctc cattcattca
     1021 ctcacctcgg cggtgagact gcagagccgg gcttcgccga gccgtttatt caaagtatcg
     1081 cagaccggct caaccaaagc ggttaccgcg ttcaaatcac cccattcggt tatttctgcg
     1141 agtgggattt atcagtctat ggtgaaagcc ttgccaaagt ttggaaagaa atacattaga
     1201 gcgctgctcc ggaaactctt ctccgccagc tccggccact tcggtcgaaa cgtgctataa
     1261 tcgcgcaccc ataaaccgga ggagcacatg aagctgataa gaagcaagat tttctcgttg
     1321 acgttcttga tgatctgttt aactgttttc ggctgcagaa agggcgagga gctcgctgct
     1381 cccgacgacc ttccggatat cagcaaacaa atccgccctt acgtcacggt cgcaagcaca
     1441 aaggtccgca ccgggccggg cccgcagttc agagctactg gcgaagtgcc gccccgagcg
     1501 aaggttcacg tggtcggcag agatggagac tgggtgctga tcgtgtcgaa gaaaggcaac
     1561 attccaggtt tcatcgaaat ggcttccgtc aaaccaggtg aaggcgaaga gagcgcaacc
     1621 ggcgatccgg cagtgggcgg aaagtatgaa atcctcgcca ataccaagct gtatagcggg
     1681 cccgggcttc attacccagt ggttgcggag gtgtcaaaag ggacgattat caacgtcgtc
     1741 gaagaagaca aaggctggat aaaggtagag tcaaaacggg gcaagcaacc tggctatgtc
     1801 gaatcgagct tggccaaacc ggcggcggat caatagattc ctaggcaggc gaccctctct
     1861 ttcgcaaacc tccaccgaga atactcgatg atgttactaa aagtgtccag cggatctagc
     1921 tcttcaaccc gctccttcct ttgcgtggat cggtattcgc ggtgaaatct gtagcgcacg
     1981 tcgagcgctc ttggcgatca agaaccggaa cggcgacggc gtaagatcac ctacagagca
     2041 caagaagaac catgttaatc tttgttaatc ttttgcacgc ttgcaaacct ccatttagcc
     2101 cctcgctctc ctgctgaaga tcgccttaat gcttgttgtt gcactggttt ctcgcgtaga
     2161 atcgttgcca tacctgagcg gaaggagatg gtgatggaaa tcgagaggaa gttagccgaa
     2221 cttggaatcg aagtgccggc gccgccggta ccggcggctc actacattcc ctatgttctc
     2281 gtcggcaatc tcttgttcgt cggcggcaac accggccggc tcaatggcca accgcaccta
     2341 tacgccggca aagtaggcga ccacgtaacc ttagaacaag cttaccagat ggcgcgccat
     2401 tgcgccctga atcatctcgc aatcatcaag tcagccgtgg gcgatctcga tcgggtggag
     2461 cgctttgtca aattgctcgg ctttgtaaat gttgctccag gttttaccga gatgcctcag
     2521 gtggtgaatg gcgcgtccga tctgtttatc gagatctggg gggaacgagg gcagcacgcc
     2581 cgcgccgcag tcggagttgc ctctctcgcc aacgaagcgc cggtggaaac cgaggtgatc
     2641 gtgcagatca gagattagga ttgcggtcca aatctcccat caaatgtcgg gttcaagagc
     2701 agcttatgaa taatcgactg acgacttgta ttgcgcaacc cagcgggttc tcataaccca
     2761 cgcaaaaacg tcggtggaca agcgccgctg ccagctcaat caacttgatt aggctgcagt
     2821 tctatccgat ggtcgggttg gcgccgcgac atcctagctt actcccattc acgttcgctt
     2881 ggctcgcatt gtaatctcac ggatcttatg gatatgagat cactttgaag cgaactgctg
     2941 ctgtcacatc gttgccgcca cgcttaagct ttgcccgcgg ctttcaatcc ccgcagcaca
     3001 ttctcagggc ttagcggcaa cttgttgatg cgcacgcctg tggcgttgaa gattgcgttg
     3061 gcgatgcacg ccatcggcgg cacgcaagcg atctcgccgg cgcctttggc gccgtaggga
     3121 cccgctcctg gatagccggg caccagcgtc gtttcaatcc gcggcatcat gttgatcgtc
     3181 ggcatcaagt aatccacgaa cgacggattc aacgttcttc catctttatc gaagaccacc
     3241 tgctcgcaga gcgcttggcc gatgctcatg gcggtggcgc cttcgatctg accttcgacg
     3301 gcggcttgat ggatcggttg gccaatgtcg tgcacggcga cgtatttcaa aatcttcacg
     3361 ttgccggtat ccggactcac ctcgacgatg caggcgtgcg cggtgaacgg ctgcgagctg
     3421 ccatgggctt tttcttcggc catcaattca agattgtcgc agtagccgcg gccgatcact
     3481 tcgccgatga cattgtgcgc attggtcgca acggtcgcca gcgctattgc cttggccggc
     3541 gtgcctttaa cccggatctg cttgtcgacg atctcgagat cgtccgggct agcctcgagc
     3601 tggcgcgcgg ccattttgaa aagctggttg cgcgcgtcga tagccgccag tcgagatgcg
     3661 gtgccggaaa cgagaatgcc acggctgccg accgtgccgg catcccacgg gctggcgtca
     3721 gtgtcgacat tgaccaccgt gacgtcgctt actggaatgc cgagctcttc ggcaacgatc
     3781 atgggaatac cgaaaccggg gatggtgccc atgtcgatct tgccaatatg caccgtcacc
     3841 gagccatctt cgttgacttt tacaatggca ccgccgccgg gacctgcact ttcgatccat
     3901 gacccggtgg cgagtccgat accctggttt ttttccagct ttacgtttcc ccagccgatc
     3961 ttctccttgg cgatgcggat cgtttcaccc aacgaaacat cgcgcagctt gggcacgccg
     4021 acgatcttgt ccccggcttt gggaacgttc ttcaggcgga attcaaccgg atccatgccc
     4081 agcttggcgg caatggagtc catatgcgac tccacggcga aggcggcctg cgggccgccg
     4141 gggccgcgca ccgggccgca gacctgcttg ttggtgtaaa cgctgtaacc ctcgatgtgg
     4201 caattgggca tcaagtacca gccgcctagc atgttggtgc agcggccgcc ggcctgcacg
     4261 ccgaaaccgg agtagccgcc ggcgtcgtaa aataccttgc cggtacgggc gacgatagtg
     4321 ccatcttttt ttacaccggt cttgatgtac atccgcgcgc cggatctaat cgtcgtcgaa
     4381 atggtttctt ctgctttgtc gagaacgatc atcaccgggc gcttggcttt gatcgccaag
     4441 agtccgcaga tcggctcgac ggcggcgccc gaggtgatac cgctgccctt gccgccgaaa
     4501 cctccgccca atccggcgga gatcacgcgc acgttgttga gcggcaagcc gagcgtcttc
     4561 gccacctctt ggcgcaccgg gaagggcgcc tgggcattgg cccagacggt gagacggccg
     4621 tttggctcca tctgcgccgc ggcgatgcgc ggctcgagat actgcggatg gatcatcttg
     4681 gtttcgtaaa cttcatcgac tacgagatcc gcttcggcca tcgccttttc ggcatcgcct
     4741 atatgaacga cggtatggcc gcagatattt ttaagccctg catccgcggg gattgttttg
     4801 ggcaacggca tgtcatcgtg caacgtcggt gcgtccgcct tgatcgcttc ctccggatcg
     4861 atcaccaccg gcagcggctc gtagacgacc ttgatttttt tgagcgcttg tctggcgatc
     4921 gccgacgatt cggcggcgac ggcggcgacc ggatcgccca taaatctgac tttgccgcgc
     4981 gcgagaatat ggcggtcgat gtaactgcgg ccataacgca cgttcggcgc atcctccgcg
     5041 gtcaccacgg cgtgcacgcc gggaattttc ctggcttcgc tggtgtcgat gctgacgatc
     5101 ttcgcgtggg cgtactggct gcgcagaatc gcgccgtgca gttggtcttt gaacatcagg
     5161 tcgatgccgt agacgccctt gccgacgacg cgctcgatcc cgcccatgcg ggctgggctc
     5221 gagccgatga ttttgagttc cggtttggcc atgacttaag ccctccctgc gatgcgatcg
     5281 gcggcgagat gaatcgcgtc gacgatcttg ttgtaaccgg tacagcggca gatattgccg
     5341 gagaccgctt cttcgatgtc ttccgccgtc ggctttgtcg tgcgatcgag caacgctttc
     5401 accgaaatga tgtatcccgg cgtgcagtag ccgcactgca cggcgccggt gtcgatgaac
     5461 gcctgctgca ccggatgcag tttatcgcca ttggctagcc cttcgatcgt gatgatctcc
     5521 gcgccgttcg cgtcggcggc catcgtaacg caggagttga tcggcttgcc gttgtagatc
     5581 acggtgcacg cgccgcactc gccggtgttg cagcaaagct tgctgccctt catttccaaa
     5641 gtgtcgcgga gaaactccag gagtgaggtc gacgcatccg cgctagagct gactgcctgg
     5701 ccgtttattt tcgtgttgat cgtgacgctc atttacgcta cctccttacc ggttacggct
     5761 ttccaggttt gttctagacc tcgccgggtc aacgcctcaa cgatccagcg ccgattctcc
     5821 gcgctgctgc gctgatcgtc gattggactc gcttccgtcg cgcctagcgc gccggcttct
     5881 tttaatagtg ccgccgtcag cggtttgccg cgcagcagcg cttcggtctt tggcgcgcga
     5941 accggggtcg gcgccaccgc gcccaatccg atgcgaacat cttgtatatt gctcttggca
     6001 gcatccggcg tgatcaacac tgccacgcca accatggcga tatccattgc gccgcgcacg
     6061 gcatgcttca aatagacgcc tttgctgccg gcagggggat acggaacgtc gatttcagtc
     6121 agcagaccct tgccgccgag gacagacttt cccggtccct gaaaaaactt catgaacggt
     6181 tggttttgtt cgccttccga aaaagcgcac ttcgcgctgg cttcgagcac cagcagcggc
     6241 gccaaggttt cgccggaggg cgaagcgcga cagatgttgc cgccgattgt ggcggtgtta
     6301 cgcacctgta aagacgcgaa ttgatgcgcc gcttcccaga gcacgggtag cttatcgcgc
     6361 agaatagtgt tggtctccag ctcgcggata gtcaccaatg gcccgacgcg caggctgccg
     6421 ccgttctctt ggatacccga tagtccggga agctttttga tgttgatcag gcaggccggc
     6481 tcttccttat acttgatgtc caccatcacg tcggtgccgc cggcgatgat tttcgcttcc
     6541 ccgccatgct tggccagcag cgacaccgcc tcgttcaccg ttttcggttg gtaatattcc
     6601 atagtctctc cgatcgcttc ctgcggaatt tcgatcagag tttttcatag cccaagtgta
     6661 agattgatcg caagggccca
//
LOCUS       DASUTG010000033         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2080477, whole genome shotgun sequence.
ACCESSION   DASUTG010000033 DASUTG010000000
VERSION     DASUTG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080477"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VF089_00720"
     CDS             <1..869
                     /locus_tag="VF089_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6172505.1"
                     /translation="LLREERIVGALVVRRKSIGEFRSEVVDLMKTFATQSVLAIQNAR
                     LFREIEDKSRQIEAANRHKSEFLANMSHELRTPLNAIIGFSEVLLDPAMPVTDEERLQ
                     FLTDVLASGKHLLSLINEILDLAKVEAGKMELQIEPALLQDVIDAVSNTMRPLAVKKS
                     IDLRAQCDERLAPIAMDGARIKQVLLNLVGNAVKFTPDTGKVWVRANAADDEVQIEVS
                     DTGPGIPPADRERIFLEFQQVGRDAGKPQGTGLGLALAKKFVELHGGRIWVESEAGKG
                     SRFTFTLPIHAA"
     gene            962..>3144
                     /locus_tag="VF089_00725"
     CDS             962..>3144
                     /locus_tag="VF089_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6172506.1"
                     /translation="MPTTKRPPSKTRSPSLKAANAQLRRENTELRRKLQARDRDLGES
                     LEQQSGTSDILRMIARASGDLQSVLDAIAESAARLCSADDAIVWRVSDNVLVLAAHFG
                     PIPTVVVEGGGHVIDRDTPAGRAVVDGQTTHVHDLVAAASDFPRAKTRGIAVGVRTAL
                     AAPLLREGNVIGAIHIRRTEVCPFSEKQIKLLETFADQAVVAIENVRLFGELGERNAE
                     LREALEHQTATSEVLGIISRSPTDVQPVLDAIVESAARVCGIDDVVLRLHEGNSAVTR
                     AHFGPIPIGRVEISSDDPRFDWMREYGTLHIPDVLDQNDFPMLGSSGRWRTFLAAPLR
                     QQGEVIGSLGARRIEARPFTHAQIKLLETFASQAVIALENVRLFQELKEALEQQTATS
                     EILGVIASSPTDIQPVLDTIAESAARVCNANDASIRLVEGNMLRLAAHHGPIPFFAVS
                     ELPIDRDSMAGRALIERQIIHIEDLLSIVATEFPRTQSFTEQEGTRTALVVPLMREDV
                     PIGTILIRRTEVCPFADKQIALLKTFAAQAVIAIENVRLFKELQERNAELREALEHQT
                     ATSEVLGIISRSPTDVQPVLDAIVESAAKVCGIDDVVLRLREGNNLVSRAHFGPIPVP
                     ANRIEIDIDAPQYRWIREHGTLHVPNISEWNDFQFVRAPSWRSRMTAPLRQQGEIVGV
                     LNARRIEVRPFTPAQIKLLETFADQAVIAIENVRLFNELKEALEQ"
BASE COUNT          692 a          882 c          939 g          631 t
ORIGIN      
        1 cgctattaag ggaagagcgc attgtcggtg ccttggttgt gcgtcgaaaa tcgattggcg
       61 agtttcgttc cgaagtagtc gatttgatga agaccttcgc gacacagtcc gtattggcga
      121 tccagaacgc ccgcctgttc cgcgagatcg aagacaaaag ccgacagatc gaagccgcca
      181 accgccataa atctgaattc ctcgccaaca tgtcgcacga actgcgcacg ccgctcaacg
      241 cgatcatcgg cttttccgaa gtgctgcttg atccggccat gccggtgacc gacgaggagc
      301 ggttgcagtt cctgaccgat gttttggcca gcggcaagca ccttctcagt ctaatcaatg
      361 agattctcga tctggccaag gtcgaagccg gcaaaatgga actgcagatc gagcctgcgc
      421 tgttgcaaga cgtcatcgac gccgtttcca atacaatgcg gccgctggcg gtcaagaaat
      481 ccatcgacct tcgcgcccag tgcgacgaac gtttggctcc gattgcgatg gacggtgccc
      541 gaataaagca ggtgcttttg aatcttgtag gcaatgcggt caagttcact ccggacacgg
      601 gcaaagtatg ggtccgggct aacgccgcgg acgacgaggt gcagattgag gtgagcgaca
      661 cgggaccagg tatcccgccg gcggatcgag aacggatttt tctcgaattt cagcaagtgg
      721 gcagggacgc tggaaagcct caaggcaccg gactcgggct cgcgctggcg aagaagtttg
      781 tcgagctgca cggcggaaga atttgggtgg agagcgaagc ggggaaaggc agtagattca
      841 ccttcaccct acccatccac gcagcgtaag gcgtgaaggg taaggagtta ggcgttggaa
      901 aagaaaattt actctcgcaa aggcgcaaag cggccaaggt tgagaacaaa ataggtaacg
      961 gatgccgaca accaagcgac cgccgtcgaa gacacgaagc ccttccctga aggcggctaa
     1021 cgcccagcta cgccgggaga acacagagct acggcgcaaa ttgcaagcac gcgaccgcga
     1081 tcttggcgaa tctttagaac aacaatcggg caccagcgat atcctgcgca tgatcgcgcg
     1141 cgcgtccggt gatctgcagt cagtcttgga cgcgattgcc gaaagcgctg ctcgattgtg
     1201 cagcgcggat gacgcgattg tttggcgagt gagcgacaac gttttagtgc tcgctgctca
     1261 cttcggaccg atacccacgg tcgtggttga gggcgggggg catgtcatcg atcgtgacac
     1321 tcctgccggg agggcggtgg tcgatgggca aaccacccac gtgcacgatc tcgtggcagc
     1381 cgcctctgat tttccacgcg ccaagacccg tggaatcgcg gtgggcgttc ggacagccct
     1441 cgcggcaccg ttgttacgcg aaggaaatgt cattggagcg atccacatcc gccgcacgga
     1501 ggtttgtcct ttctccgaaa agcaaatcaa gctgctcgaa actttcgccg atcaagccgt
     1561 agtcgccatc gaaaatgtgc gactcttcgg ggaactcggc gagcgcaacg cggaactgcg
     1621 cgaggcgctg gagcatcaga ccgcaacatc cgaggtgctc ggcatcatca gccgctcgcc
     1681 caccgacgtg cagccggtgc tcgatgccat cgttgagagc gcggctcggg tctgtggcat
     1741 tgatgatgtg gtactgcgac tccacgaggg gaacagtgcg gttacacggg ctcattttgg
     1801 tccgataccc atcggccgcg ttgagatcag tagtgatgat ccaaggtttg attggatgcg
     1861 ggagtacggc acactgcaca tccctgatgt cctcgatcag aacgatttcc caatgttggg
     1921 ttcctccggt cgctggcgca ccttcttggc cgctcccctt cgtcagcagg gcgaggtcat
     1981 tggatctctg ggcgcgcggc gtattgaagc acgtcccttt acccatgcgc agatcaagct
     2041 attggagacg ttcgcttcgc aggcggtcat cgcccttgag aacgtccggc tgttccagga
     2101 actcaaggaa gcgctggaac agcaaacagc gacgagcgaa atcttaggcg tgatcgccag
     2161 ctcgccgacg gacattcagc cagtgctgga tacgattgcg gagagcgccg cgcgggtgtg
     2221 caatgctaat gacgcttcga tccggcttgt cgaaggaaac atgcttcgtt tagcggcgca
     2281 ccacggcccg attccgtttt ttgcggtgtc cgagcttccg atcgaccgcg attcgatggc
     2341 cggtcgggcc ctcattgagc gccaaatcat ccatattgag gatctgctgt cgattgtcgc
     2401 aacagagttt ccccggactc agagcttcac cgaacaggaa ggtactcgaa cggcgctggt
     2461 cgtgccgctg atgcgcgaag acgttccgat cgggacaatt ctgattcgcc gcacagaggt
     2521 ttgtcctttc gcggacaagc agatcgcgct tctgaaaacc ttcgccgcgc aagcggtcat
     2581 cgctatcgag aatgtacgcc tcttcaaaga gttgcaagag cgcaacgcag aactgcgcga
     2641 ggcgctggag catcagacgg caacatcgga ggtgctcggc atcatcagcc gctccccgac
     2701 tgacgtgcaa ccggtgttgg atgcgattgt cgagagcgca gcaaaagttt gtggaattga
     2761 tgacgtggtg ctgcggctcc gtgaggggaa caatctagtc tcgcgggcgc attttggtcc
     2821 catacccgta cccgcgaacc gcatcgagat cgatattgat gcgccacagt accgctggat
     2881 ccgtgaacat ggcacgctcc acgttcccaa tatcagcgag tggaacgatt tccaattcgt
     2941 gcgtgctccc agctggcgca gccgcatgac cgctcccctt cgccagcagg gggaaatcgt
     3001 tggagtgctg aatgcccgtc gcatcgaagt tcgtcccttt actccagcgc agatcaagct
     3061 cctggaaacc ttcgctgatc aagccgtcat agccatcgag aacgtccggc tttttaacga
     3121 gttaaaagaa gcgttggagc agca
//
LOCUS       DASUTG010000034         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1191105, whole genome shotgun sequence.
ACCESSION   DASUTG010000034 DASUTG010000000
VERSION     DASUTG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191105"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VF089_00730"
     CDS             complement(<1..811)
                     /locus_tag="VF089_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6172507.1"
                     /translation="MNLQDQPPLDERVRRLTLINSVIGVTIAGISTRVFMISVPTLAT
                     ALGTDILGISWALIVYQMAGIGLGEICGRLGDIYGHHKMFGSGMGIMALGSLLCGLSQ
                     DVLQLILFRFVQGVGGAMIQSSGRTLAFRAMPAGSEGKAQGLMAMSHQFGFFIGPPIG
                     GLIIDLVHWRGIFFLLFVPSLVGMALCFVTGRSVAASSSRRQAIDYRGAAIFLGLTVL
                     VTMLLDQKIVEVLGGGSQALLTVTFVGALWGFVAHERRTPSPMIDLSFFSVP"
     gene            1286..2305
                     /locus_tag="VF089_00735"
     CDS             1286..2305
                     /locus_tag="VF089_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX6172508.1"
                     /translation="MTKSALIVLGLLFLAPTSAAAQVKDLSWGTSAVGTSGHRALVTL
                     AALLNREMKGYRIAVLPTPGAIATVKGYATGQFDGYYGSDIAFHEMAGDTERFKGFRA
                     QMKREPVQSFWAFTLEVGLGILARDMKKFKQWRDLSGERIFTGPLPFDTRAATERAMG
                     LAGLKHKYVEVDLAVAGSALDRGDIRALTIYTAGHTTLPPWLVEAQLGTDIAVLNPSK
                     AEIDMIRKGGMQVVEVSPKVFKKSVHVDNMFLVPFYYGFHVGLEVPENDAYTMLTIIE
                     KHLAELVKSDAAYSQIKDAMPQFQRSGVESSVNDVLVHPGLARYMRERNVWDTKWDKR
                     IAKGK"
     gene            2440..>2851
                     /locus_tag="VF089_00740"
     CDS             2440..>2851
                     /locus_tag="VF089_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX6172509.1"
                     /translation="MGPTEERPNKWPTIFFFLFAFSFFVYLLRYYLTGDGGPTLLAVT
                     LVPVTFILFILDALRTGELYPQLGRTANYILGTVYIGLSVLVAVYFNVEFFDVRTVRL
                     GIWNQYDLLVGAVMLILVMEYCRAKYFALFLINLI"
BASE COUNT          649 a          777 c          798 g          627 t
ORIGIN      
        1 caggcacgga gaaaaaagac aggtcgatca tcgggcttgg ggttcttcgc tcatgcgcaa
       61 cgaaacccca gagcgcgccc acgaacgtta cagttaaaag cgcctggctg ccgccgccca
      121 acacctcgac gatcttttga tcgagaagca tcgtaaccag caccgtaagg ccgaggaaga
      181 tcgccgcccc gcgataatcg atcgcctggc gtcgcgaact ggaggccgca actgagcgtc
      241 cggtgacaaa gcagagcgcc atgccgacca ggctcggcac aaacagcagg aaaaaaatgc
      301 cgcgccaatg aaccagatcg atgatcaggc cgccgatcgg cgggccgata aaaaacccaa
      361 actgatgcga catagccatc aatccctggg ccttgccctc ggaacctgca ggcatcgcgc
      421 gaaacgccag ggtacgcccg gaagattgaa tcatcgcgcc gccgactcct tgcacaaaac
      481 gaaacaggat taactgtaaa acatcctgcg atagcccgca gagaagcgag ccaagggcca
      541 tgatgcccat gccggagccg aacattttgt gatggccgta gatatcgccc aaccggccgc
      601 agatctcgcc aagtccgatc cccgccattt gataaacgat caacgcccag gaaatgccta
      661 gaatgtcggt tcccagagcc gtcgccagtg tcggcaccga aatcataaac actcgggtcg
      721 aaattccagc tatggtaacg ccgatgacgg aattgatcag agtaagtctg cgcacccgtt
      781 catcgagcgg cggttgatcc tgcaaattca cagttgtgag cctctcctaa gacgttacgt
      841 ggaattgtca aactctccgt aacacttgtg caacgtgttg cgatgacttg taatgctata
      901 gcgaacggct gagatcgttc gacaataacg actcccagct ctctttggta ggactggaga
      961 ggatctaaca aggatttttc tcggagttta ccctgagctt ggccgaaagg ttcgaaatca
     1021 taacggtgcc cttgtgtctt tggcgagttg gtgatttctg tgaccgcttg tttgggcagg
     1081 agtgcctccg tcaaaccggc gagatcaacc gtacctcctt cgagtttttc ccggaagttt
     1141 ctcaatggtt agataaacaa tctgcagccg tcgcccactc tctagctctc ggattccccg
     1201 tgggggttgc ggttgacacc catgcacggt ctaaactaaa tagactcgtt cccattgcgc
     1261 caaccagaag acgaagggag ctgtcatgac aaaatctgcg ctgatcgttt tggggttact
     1321 ttttctggcg cctacgtctg ccgccgcgca agtaaaggac ctttcatggg gaacgtccgc
     1381 cgtcggcacc tccggtcacc gggcgctggt caccctggca gcgctgctca accgggaaat
     1441 gaaaggttat cggatagcgg tcctgccgac gcctggagcc attgctaccg tgaaaggata
     1501 cgccaccgga caatttgatg gttactacgg ctcggatatc gcctttcacg aaatggcggg
     1561 ggataccgaa aggtttaaag ggtttcgggc gcagatgaaa cgggaaccgg ttcaatcgtt
     1621 ttgggccttc accctcgaag ttggtctggg tattttggcc cgcgacatga agaagtttaa
     1681 acaatggcgc gatttatccg gcgagagaat tttcactggg ccgctgccgt tcgatacgcg
     1741 cgccgcgacg gaaagagcca tgggtctagc ggggctaaag cacaagtacg tggaagtcga
     1801 cctcgcggtc gccggatcgg cgctcgaccg cggggacata cgggcgctca cgatctatac
     1861 cgcgggccac acgaccttgc ctccctggtt agtcgaagcc cagctcggca ccgatatcgc
     1921 ggtgttgaac ccgagcaagg ccgagatcga tatgatccgc aaaggcggca tgcaggtggt
     1981 ggaggtgagc ccgaaggtct tcaagaaaag cgtccacgtg gacaacatgt tcctggtgcc
     2041 gttttactat ggctttcacg tcggcctgga ggttcccgag aacgatgctt acacgatgtt
     2101 gaccatcatc gagaaacatc tcgccgagct tgtaaaatcc gacgctgcgt actcacagat
     2161 caaggacgcg atgccgcagt ttcaacgcag cggcgtagag tccagcgtca atgacgtgtt
     2221 agtgcatccg ggtctggcga gatacatgag agaacgcaac gtttgggata ctaaatggga
     2281 taagcgcatc gctaagggca agtgaccttt gcgttagccg gcacaggttt gttttacggc
     2341 tttggggcag gcaagcgcag gccttagaca aaatgcccgt gcggctcggc gcgttgtcca
     2401 cgacccgttg cgacggattg gaaaggagtt ggctcaagcg tgggtccgac tgaggagcgc
     2461 ccgaacaagt ggccaaccat tttctttttc ctcttcgcat ttagcttttt cgtttatctc
     2521 ctgcgctact atttgactgg ggacggcgga ccgacgctgc tggcggtcac tctggtgccg
     2581 gtgactttca ttctttttat tctggatgct cttaggaccg gtgagctcta cccgcagtta
     2641 ggacgaactg cgaattatat cctgggaacc gtttacattg gtttgtcggt cctggtcgcg
     2701 gtctatttca acgtcgagtt cttcgacgtt cgcacagtcc ggctgggcat ttggaatcag
     2761 tacgatctgc tcgtcggcgc tgtcatgttg attctcgtca tggagtactg ccgagccaaa
     2821 tactttgcgc tgttcctgat caatctgatt c
//
LOCUS       DASUTG010000035         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_153531, whole genome shotgun sequence.
ACCESSION   DASUTG010000035 DASUTG010000000
VERSION     DASUTG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153531"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1521
                     /locus_tag="VF089_00745"
     CDS             49..1521
                     /locus_tag="VF089_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX6172510.1"
                     /translation="MSLKQSIGLGVVVFWCVMNILLIKRQIWAPPPPLFVSATETLTE
                     ASQEWWGVYYHGEKIGFTSQTIEPKQNGYELRDESLLRLNLLGTTQRASTRLAMTVDK
                     EWVLKDFEFELLSNDVRFQSRGKVIPGQLNLEIESAGHTSKQIIALTQPPYLAAALKP
                     LVATQQLEPGKEYFFTIFDPATLAHQITSVVIEAREQILLNGQKVPAIRLRQRFKGIS
                     VISWIDGNGLTLKEESPGGFALRQEAPPQAKSFADSRAIPLDLIARASITVGESISQP
                     QTRSTLLLRLSGVKLVDFALDGERQKLSENVLRIEREQVSPSDTYKIPLTDRRFRSFL
                     QPTAFLQSDHPYIRDHAVQILGAETDAHRAVLRLKDWVYDNLKKQPTISIPNALQVLQ
                     TKTGDCNEHTVLFNALARGAGIPARTVVGMVFLRGAFYYHAWSEVWLGKWVSLDSVLN
                     QFPADVTHVKFIEGEIDRQLDMLQLIGNLRIEVPEQESFQ"
     gene            1631..2389
                     /locus_tag="VF089_00750"
     CDS             1631..2389
                     /locus_tag="VF089_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6172511.1"
                     /translation="MIRIINLRKQYGRLAAVDNLNIEVPPGEIFGFLGPNGAGKTTTI
                     RVMMGILRASSGRVILGGHDVEQEPQQAKAIAGFIPDRPFIYEKLSGKEFLTFVAKLH
                     RMESARRKRRIDELLEYFELSNWQDELVEGFSHGMKQRLVLCAALVHEPRILIVDEPM
                     VGLDPKGARTIKNLFRSLAEAGTTVFLSTHSLSVAEEVCHRIGIIQKGHLIASGSMAD
                     IYRLSRGHDSNLEDVFLELTQQQDEFTSSRERRL"
     gene            2386..4077
                     /locus_tag="VF089_00755"
     CDS             2386..4077
                     /locus_tag="VF089_00755"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172512.1"
                     /translation="MSIILLLLSPKWMTWKNDFHRTRARSARRWSLLALGICFWIGTF
                     YVIRRVLMYFQSVHELGSALAYQLLLIILLTFLSMLLFSNLITALSAFFLARDLDLIM
                     SMPLSHNGFYYSRLLMTMVNSSWMVLFFSLPIFAAYNAVFGGGFIFYLWLMLCLPLFL
                     IIPAAFGVLATHLFVYFLPAKRIRDILFFVGLFAFILIFFLFRFSQPERLVRPESFGH
                     FVQFLTAMETPSSPYLPSSWSAEILAGTLFNRSTDQAFFFALMASYGLFFPLVTSWVS
                     GAVYLDGWSKAQESNQGRRRLKSVDLLIDLVTRPFSGGMRAILIKDIKTFMRDSSQWS
                     QVFLLVALIVVYLYNFKVLPLDRSPVPAGTLRTIVAFANLALAGFVLSAVAMRFAFPA
                     ISLEGNAFWLLQTSPISLRTLLWSKFWLNVLPLLFLGELLVFLSNLMLGVPNWMMILS
                     LVTIFLMTFGITAICVGVGAIYPKFSYDHVAEIPTSFGGAICMIFSIGFIGLIVMIEA
                     WPIYLLTSRSPGSGGSHTSIAWVIAPSLMAAIALTVVAVVVPIRKSLKNLEAMRA"
     gene            4271..4918
                     /locus_tag="VF089_00760"
     CDS             4271..4918
                     /locus_tag="VF089_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6172513.1"
                     /translation="MELIHTLYQPAGKGPHPTILTLHGRGASAFDLLGLAPYLCGGRF
                     LIICPQGPLETPIGPEASGYAWYPMSMGGPPDIASMMRSRQRLQQFLDECLAQYPIDA
                     KKLAILGFSQGGVMAYSLALSAPDRFAGLAALSSWLPPELVPHLTLSNSAQALPTLVQ
                     HGTQDSMIEIERARKSVETLRQARIPLTYREYEMGHEIRPRSLNDLSAWLEEKVL"
     gene            complement(4947..5822)
                     /gene="htpX"
                     /locus_tag="VF089_00765"
     CDS             complement(4947..5822)
                     /gene="htpX"
                     /locus_tag="VF089_00765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX6172514.1"
                     /translation="MNMLRTTILLALLTALLVWAGGILGGQQGAVIAFVIAAAMNFFS
                     YWFSDRIVIKMYQGQEVTAQDDPELYGIVENLAQRAGLPMPKVYMLPQETPNAFATGR
                     NPQHAAVAVTVGIRRILDKRELAGVLGHELAHVGNRDILVSTIAATLAGAISYLATMA
                     QWAAIFGGHRDSREGGGGMISLLVMMIVAPLAAMLIQMAVSRSREYGADVGGAKTTGD
                     PLALASALRKLHLGSQRIPLHTSDATANATAHMFIVNPLTGHGLASLFSTHPPMDERI
                     ARLEAMAKDHSYMLR"
     gene            5979..>6136
                     /locus_tag="VF089_00770"
     CDS             5979..>6136
                     /locus_tag="VF089_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6172515.1"
                     /translation="MRIGVVFPQTEIGTDAAVIREFSQAAEQLGYRHILAYDHVLGAN
                     LGSRPDWRP"
BASE COUNT         1358 a         1644 c         1640 g         1494 t
ORIGIN      
        1 gtttgaactg atttcccatt gcatttacca tagccgtgtt cttaccgcat gagcttgaaa
       61 caatccattg gcctcggtgt cgttgtgttc tggtgcgtga tgaacatcct gctcatcaaa
      121 cgccagatct gggcgccgcc gccgccgctt ttcgtcagcg cgacggaaac tctcacggaa
      181 gccagtcagg aatggtgggg ggtatactat cacggcgaga aaatcggctt cacttcgcag
      241 actatcgagc cgaagcaaaa cgggtatgag ttgcgcgatg aatcgctgct gcggttgaac
      301 cttttaggaa cgactcagcg cgcttcgact cgactggcga tgaccgtgga taaagaatgg
      361 gtgcttaaag atttcgaatt tgagctgctg tcaaacgacg tccgtttcca atcccgtggc
      421 aaggtgattc cgggtcagtt aaaccttgaa atcgagtcgg ccggccatac atcgaagcag
      481 ataatcgcgc tgactcaacc gccgtactta gccgcagctt tgaaacctct agtggcaacc
      541 cagcagttgg agcccggcaa agaatatttc tttacaatat ttgaccctgc gactctggcc
      601 caccaaatca cttcggtggt catcgaagcg cgcgagcaga tcctacttaa cggtcaaaaa
      661 gtgccggcga tcagattgcg tcaacgattt aaagggattt ccgtcatctc atggatcgat
      721 ggaaatggcc tcacgctgaa ggaagaaagc cccggcggat ttgctctgag acaagaagcg
      781 ccgccacagg cgaagagctt cgcggattcg cgcgcgattc ctttggatct aattgcacgc
      841 gcttcgatca ccgtaggcga gtcgatctct cagccgcaaa ctagaagcac cttgttattg
      901 agactaagcg gcgtgaagct ggtggatttc gcattggatg gcgaacgcca gaagttaagt
      961 gagaatgtat tgcgcatcga gcgagagcag gttagtccgt ccgacactta caagattccg
     1021 ctgacagacc gtcggttccg ctcgttctta caacccacag cattcctgca gagcgatcat
     1081 ccgtatattc gcgatcatgc cgtccaaata ctcggcgcgg aaaccgacgc gcacagagcg
     1141 gtactgcgcc tcaaagactg ggtctacgat aaccttaaaa aacagcccac cataagtatc
     1201 cctaacgctc tgcaagtgct tcaaaccaaa accggcgatt gcaacgaaca tacggtgctg
     1261 ttcaacgcgc tcgcgcgcgg cgcgggcatt ccagctagaa ccgtagtcgg catggtcttt
     1321 ctgcgcggcg cattttacta tcacgcctgg tccgaagtgt ggctcggcaa atgggtctcc
     1381 ttagactctg tgctcaacca gtttcccgcc gacgtcactc acgtcaaatt catcgaaggc
     1441 gagatcgacc ggcagttaga tatgctgcag ttgatcggca atctcaggat agaagttccg
     1501 gaacaggaat cattccagtg agaccggcag gactcttttc aattcctgat gcgccaatcc
     1561 ccaagccgcc tttcgcctcg gataaaaacg gcttgacgtt aattgacgtt aaatagctag
     1621 aaagctctct atgattcgca taattaattt acgcaagcag tacggccgtc tggctgccgt
     1681 ggataacttg aacatcgaag ttcccccggg agaaatcttc ggcttcctgg gccccaacgg
     1741 cgcggggaag accacgacca ttcgggtgat gatggggatt ttgagagcga gctcgggacg
     1801 agtgattctg ggcgggcacg atgtcgaaca agagccgcag caagccaaag cgattgccgg
     1861 cttcatcccc gatcgtccgt ttatctatga gaaactcagc ggcaaggagt tccttacgtt
     1921 tgtcgctaag ctacaccgga tggaatcggc tcgacgaaag cgcaggattg acgaactctt
     1981 agaatatttc gaattatcca attggcagga tgaattagtg gaagggtttt ctcacggcat
     2041 gaaacagcgc ctagtattat gcgcggcgct cgtccatgag ccgcgcattc tgattgtcga
     2101 tgagccgatg gtcggcttag atccgaaagg cgcgcgcacc attaagaacc tattccgttc
     2161 attggcggaa gccggaacga cggtttttct gtcgacccac agcctcagcg ttgccgagga
     2221 agtatgccac cgaatcggga ttattcagaa agggcacctg atagccagtg gaagcatggc
     2281 ggacatttac cgactgtctc gcgggcacga tagcaatttg gaagatgtct ttcttgagct
     2341 gacccagcag caggacgagt ttacgtcgtc gcgggagcgg cggctttgag cataattctg
     2401 ctactcctct ctcctaaatg gatgacgtgg aaaaacgact ttcatcgcac ccgggctcgg
     2461 tcggcccgac gctggtcact cctggccctg ggaatctgtt tctggatcgg cacgttctat
     2521 gtgattcgtc gcgtgctcat gtactttcaa tctgtgcatg agctcggttc cgccctggct
     2581 tatcagctct tgttgatcat cctgctgacc ttcctatcga tgctattgtt cagcaacctg
     2641 atcacagcgc tttcggcatt ttttctcgcg cgcgatttgg acttgatcat gtctatgcca
     2701 ctgtcacaca atggtttcta ttattcgcgt ttgcttatga cgatggttaa ctcatcgtgg
     2761 atggtgctat ttttcagttt gccgatcttc gccgcctaca acgcggtctt cggcggcggc
     2821 ttcatctttt acttatggct gatgttgtgt ctgccgctgt tcttgatcat ccctgcagcc
     2881 ttcggggttt tggctacgca cttattcgtc tattttcttc ctgccaagcg catccgcgat
     2941 atactttttt ttgtcggact atttgcgttc attttgattt tttttctctt tcggttttcc
     3001 cagccggaaa gattggtgcg gccggaaagt ttcggacact ttgtccaatt cctaaccgcc
     3061 atggaaacac cttcttcgcc ctatctaccg agcagttggt cggctgaaat cttggctgga
     3121 actctattta accgctccac cgaccaggct tttttctttg ccttgatggc gagctatggg
     3181 ctgttttttc ccctcgtaac gtcatgggtc tcgggggcgg tttatctgga cggctggtcc
     3241 aaggcacaag agtcgaacca gggacggcga agattaaagt cagtcgacct gctcattgat
     3301 ttagtcactc gcccgttttc gggggggatg cgagctatct tgatcaagga catcaagact
     3361 ttcatgcgtg atagcagcca gtggtctcag gtattccttc tggtcgcgct gatcgtggtt
     3421 tacctctaca attttaaggt tttgccgctc gatcgatcgc ccgtgcccgc gggaacctta
     3481 agaaccatag ttgcgttcgc caatttggcc ctggcgggtt tcgtgctcag cgcggtggca
     3541 atgcgctttg ctttcccggc aataagtctg gaaggcaatg ccttctggct tctgcaaacc
     3601 tcaccgatat cactcagaac gctgctctgg agtaagtttt ggctaaatgt tttgccactt
     3661 ctttttctcg gtgagttact ggtcttttta agcaatctga tgctcggggt accgaactgg
     3721 atgatgattt tatcgctggt tacgattttt ctgatgacgt tcggtattac cgcgatttgc
     3781 gttggcgtcg gcgcaatcta tcccaaattt tcctacgatc acgtggccga aattccaacc
     3841 agcttcggcg gtgcgatctg catgatcttc agcatcggct tcatcggcct tattgttatg
     3901 atagaagcgt ggccgatcta cctgctgacc agccgatcac ccggatctgg aggatcccac
     3961 acctctatag cgtgggtgat tgccccttct ctgatggctg cgatcgctct gaccgtcgtc
     4021 gctgtagtgg tgccgatcag aaaatctctg aaaaaccttg aggccatgag agcctaagag
     4081 ccggaagcac cgggggtgtt tgcggaataa ctctccggct gcagcattga acttagaaat
     4141 ggccagaaac ccggggcggt gaccagtggc ttgtgtcgaa gtgcattgca gaaccttgtg
     4201 tcgatgtgca ttgcagaaca tggatagctc gataaagtac agggactaaa cattcgcccg
     4261 aggtcataca atggaattga ttcatacgct ttaccagcct gcgggaaaag gcccgcatcc
     4321 aaccattctg acattgcatg gtaggggcgc cagtgcattc gatctgctcg gtctcgcccc
     4381 atacctctgc ggcggtcggt ttcttatcat ttgtccgcag ggtccattgg aaacgccgat
     4441 cgggccggaa gccagcggtt acgcttggta tcctatgagc atggggggac cgccggatat
     4501 tgcgtcgatg atgaggtcgc ggcaaaggct acagcagttt ctcgatgaat gtctcgcgca
     4561 atatccgatc gatgccaaga agttggcaat cctcggtttt agccaaggcg gcgtcatggc
     4621 ctacagcttg gcgttatcgg ctccggatcg ctttgccgga ctggccgcgc taagtagctg
     4681 gctgccaccg gagctcgtgc cgcatctaac gctgagcaac tcagcccagg ctttgccgac
     4741 gttggttcaa cacggcactc aggattcaat gatcgagatc gaacgcgcaa gaaagtccgt
     4801 ggaaacactg cgtcaagcgc ggatcccgtt aacgtatcgg gagtacgaga tgggacatga
     4861 aatcaggccg agaagtctta acgacctctc ggcctggctg gaagaaaagg tgctataagt
     4921 agcttgtgac cgcgaaccga tagcccttaa cgaagcatgt agctgtgatc ttttgccatc
     4981 gcctcgagtc gggcgattcg ctcgtccatc ggcggatggg tgctgaacag tgacgccaag
     5041 ccgtggccgg tgagcgggtt aacgataaac atgtgtgctg tggcattggc ggtggcgtca
     5101 cttgtgtgca acggaatacg ttgggagccc aggtgcagct tgcgcaatgc gcttgccagg
     5161 gcgagcggat cgccggtggt tttggcgcca cccacgtcgg cgccgtactc acgcgagcgc
     5221 gacaccgcca tctgaatcag catcgcggca agcggcgcga cgatcatcat taccaaaagg
     5281 ctgatcatcc cgccaccgcc ctcgcgacta tccctgtgac cgccgaatat cgccgcccat
     5341 tgcgccattg ttgccaaata gctgatggcg cccgccaatg tggccgcgat cgtacttacg
     5401 agaatgtcac gattgccgac gtgagccagt tcgtggccga gaactccggc gagctcgcgc
     5461 ttatcgagaa tacgacgaat ccctacggta accgccacgg ccgcatgctg cggattgcgc
     5521 ccggtcgcga aggcattggg cgtctcctgc ggcagcatat acactttcgg catcggcaac
     5581 ccggcgcgct gagcgaggtt ctccacaata ccatagagtt caggatcgtc ttgggccgtc
     5641 acttcctggc cttgatacat ttttataacg atcctgtcgc tgaaccagta gctgaaaaaa
     5701 ttcatcgctg cggcgatcac aaaggcgatc acggcaccct gctgaccgcc taggatgcca
     5761 ccggcccaga ccagcaatgc cgtcagcaat gccaatagaa ttgtagttcg aagcatgttc
     5821 ataattaatc cctttccacc atatcgtacg gcatcatcaa acgatgtcaa gcaaccaaca
     5881 ttgcggaaat ttgcgcatgg actttacaaa aaacaagccc caagctacag aaaaagcagc
     5941 aaaagctcgg ctcctaatgc tgagaaggag atacattgat gcgaataggg gtggtatttc
     6001 cacagacgga aatcggcacg gacgcagccg taatccggga gtttagtcag gctgctgaac
     6061 agctcggata ccgccatatt ctggcgtacg accatgtatt gggcgccaat ctcgggagcc
     6121 gccccgattg gcgccc
//
LOCUS       DASUTG010000036         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_598595, whole genome shotgun sequence.
ACCESSION   DASUTG010000036 DASUTG010000000
VERSION     DASUTG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598595"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..987)
                     /gene="xrt"
                     /locus_tag="VF089_00775"
     CDS             complement(31..987)
                     /gene="xrt"
                     /locus_tag="VF089_00775"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HEX6172516.1"
                     /translation="MALSCALVFAYATVLQKLGKDWWNDENYSHGLLIPVIIGYILWS
                     QRERFARTARSSNTLLGMFAVALALLALWAGTAGAELFIQRLSLALMLAGVVVYFWGL
                     RLLQLLVVPLFLLLLAIPIPSIIFNKIAFPLQLFASRCAVWSMGLFDIPVLRHGNVIE
                     LMPLGARETKRLEVVEACSGIRSLMTLVTLAVVFAYFTHPRGDGSAGGGDESRPSGLL
                     SRIKTYGFWRSTILVLSAVPIAIFTNALRVSGTGVLAHYYGTRVADGFFHSFSGWVIY
                     IVAFLMLFAVGMLLDRLKPQPSGGTLRCDPSVLTVQAEGAEG"
     gene            complement(1191..2246)
                     /locus_tag="VF089_00780"
     CDS             complement(1191..2246)
                     /locus_tag="VF089_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6172517.1"
                     /translation="MAICRAISARGARAVLVFASELPTSIQQRFQEGGVLIATINYSK
                     GPAHYYRELRKVIEKNSIECVHIIFFDYFRAIGWLSKLSGVKSIIYEMQNGGVFKAKS
                     WKRKLIHARNRIMTAPYVRIIAISDFIKGQLVEGGVPKEKIVVRHLGIDTGRFKPQPL
                     QRDELAKKYSIGPDEFLLSTVSYLRPIKSPEIIVKAIGVLAERNVPVRLFVGGDGEML
                     EELQQLSVSLGVEDRIHWLGLVPDPLDLLQASDLFLLATVGEAFGLVLAESMACGVPV
                     VGSRAGAIPEVVEDGVTGLLVAPRDPVTLADGIEKLVRNRTLCQEMSRAAVARVQDKF
                     TMDRTVLETLRVYESLN"
BASE COUNT          662 a          646 c          607 g          559 t
ORIGIN      
        1 gcccaagcaa aatcaggaag cgtcgatttt tcatccttcc gccccttccg cttgcactgt
       61 gagaacagac ggatcgcacc tcaacgtccc gccgctgggc tgcggtttga ggcgatcgag
      121 caacatgcct acagcgaaca acattaagaa agccactatg taaattaccc atccggaaaa
      181 actgtgaaag aaaccatcgg cgacgcgcgt cccataatag tgggccagca cacccgtacc
      241 gctaacgcgt aatgcatttg tgaatatcgc aatcggtacg gccgacagga ccaaaattgt
      301 agaccgccag aacccatagg tcttgattcg cgatagcaaa cccgagggac ggctttcgtc
      361 gccgccgcct gcagacccgt caccgcgcgg gtgggtgaag taagcaaaga cgacggcaag
      421 cgtaaccagt gtcatcaacg accgaatgcc gctacaggct tcaaccacct caagtctttt
      481 agtttcacgg gcaccgagtg gcatcagttc gatgacattt ccgtgccgga gcacaggaat
      541 gtcgaaaagg cccatcgacc ataccgcgca ccgtgaagcg aaaagctgaa gtgggaaggc
      601 aatcttgttg aaaatgattg acggaatcgg gatggcgagc aatagaagga acaacggtac
      661 aaccagtaac tggagcagcc ggaggcccca gaaatagacc actacaccag ccagcatcaa
      721 cgccaaagaa aggcgctgaa taaacagctc tgcgccagcg gttccggccc ataaagcaag
      781 cagcgccaag gcgactgcga acatgccgag cagcgtgttc gaagaacggg ccgtgcgtgc
      841 aaatcgttct cgttggctcc ataagatgta gccgatgatg acgggtatca gcaggccatg
      901 cgagtagttc tcgtcattcc accaatcttt tccaagcttc tgaagcacgg tggcgtaagc
      961 aaaaaccaac gcacaactta aggccacaat cttccaaaga ttgccgcgta gggttgcagt
     1021 agtttgcgag tttgagatga tgctcgtgac actcattggg ttggaattga agcctcgtat
     1081 ggaaagggca attcccaaac gaaactattt ttgcagattt tcaagcggcc gctacagaag
     1141 gcccttttat attaaaggat taaatgcggc gggtttccga agagggatgg ttaattgagt
     1201 gattcgtata ctcgtaacgt ctcgagcaca gtacgatcca tggtgaattt gtcttgcacg
     1261 cgagcgacgg ccgctcgcga catttcctga cacaaagttc tattgcgcac aagtttttca
     1321 attccgtctg cgagtgtgac aggatctcgc ggagcaacga gtaaacctgt aactccgtct
     1381 tcaacaactt cgggaatcgc cccggcgcgg ctccctacta ctgggacccc gcaggccatc
     1441 gattccgcta acaccagacc aaaggcttct cctaccgttg ccagcagaaa taaatccgac
     1501 gcttgaagca aatcaagcgg gtcgggtacc aggcctaacc agtgaattcg gtcttctacg
     1561 cccagactca cgctcagttg ctgcaactct tccaacatct cgccgtcgcc cccgacgaaa
     1621 agacgcacgg gcacatttcg ttcggcgaga acaccaatcg cctttacgat aatctcagga
     1681 ctcttaatgg gccgcaggta cgataccgta gaaagtagaa actcatcggg gccgatcgaa
     1741 tattttttag ctaactcatc gcgttgcagc ggctgtggct taaaccgacc ggtatctatc
     1801 ccgagatgtc tgactacaat tttttctttt ggcacaccac cttctaccaa ctggcctttg
     1861 atgaaatctg agatcgcgat gatgcgaaca taaggagctg tcatgatgcg gtttcgcgca
     1921 tgaattagct tgcgcttcca cgactttgct ttgaagacgc cgccattctg catttcataa
     1981 attatcgact taaccccact aagttttgat aaccagccga tggcgcggaa gtaatcgaaa
     2041 aaaattatgt ggacgcattc gatggaattt ttttcaatta ctttccttag ttctctgtag
     2101 taatgcgccg ggcctttcga atagttgatc gttgcaatta aaacaccgcc ctcttgaaaa
     2161 cgctgttgga tagaggttgg gagttctgac gcgaaaacca atacggcacg agctcccctg
     2221 gcggagatag cgcggcaaat tgccaagtgt cgatcttcct gactgctcca cgtacgcgga
     2281 gccgaatcaa gtaggaatgc aatgttagga atgttcatgt gataaatgga taagggaggg
     2341 ccttatgaac tgacaccgta gagctgtaga agtttttgaa cgttctgcct taaatcgaat
     2401 tttttacgaa caatagcagc acctgcattg cccattcttt ttctcagttc cggattacga
     2461 gctaatttcg caaa
//
LOCUS       DASUTG010000037         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2080998, whole genome shotgun sequence.
ACCESSION   DASUTG010000037 DASUTG010000000
VERSION     DASUTG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080998"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..1011)
                     /locus_tag="VF089_00785"
     CDS             complement(343..1011)
                     /locus_tag="VF089_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX6172518.1"
                     /translation="MTKNYAAGPDKDFSDRMEPLGRVIGRTRYVVLLAVIAVLLVALS
                     LFLLGTILAVMGIWEAWAALLHGQIEGTDLTVEFLEIVSVMLKAVFFYLIGVGLYSLF
                     IAPLNIATSLGVETLNDLETKIISVVVVILAVRFLEEFISGKQALEILQYGGALAVTV
                     GALVLFQFYNHRAKEDQQEHGTAEQSKKDLFERDQEHDQSRPGESQTSKTGEADRVSF
                     NRSD"
     gene            complement(1317..2273)
                     /locus_tag="VF089_00790"
     CDS             complement(1317..2273)
                     /locus_tag="VF089_00790"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172519.1"
                     /translation="MKRTILAFVAFAALMLIVSLPQLSSAKIVVGLSSVNVAFLPVYV
                     AQEKGFFKDAGLEVLMVMFNAGSTNLQALAGGDIQIMGSAFVETVGGRAAGMDIKNFW
                     GICNLMPFQLYSQPDFKSMKEAKGKRFAISRFGSLTDFLKRATLRHFGLDTKDVTILQ
                     IGSTPARYAALTAKAVDASIVWFPVTEIAKSQGYTKLLDLKEVFPEWPYETFAARESW
                     LKTEKDQVTKFLQAYQKGVKYTRDNKTDSVKIMQKYVKMDPAYAPAGYDEYRDSFPIN
                     GQIAEKVIPQVIAEEAATGRIKRKISLDELIDRSFIQAIGGK"
     gene            complement(2299..>2875)
                     /locus_tag="VF089_00795"
     CDS             complement(2299..>2875)
                     /locus_tag="VF089_00795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6172520.1"
                     /translation="HYDGTAFKLKNVSVDPKPLQKPRPPIWIAANSDAAVARAATIGD
                     GWMIGPHSAIDELQRQVALCRRTWSAAGKPGLPDIPIIRETFVAKTRKEATEKARPCL
                     EQLYRTIYVKWKQNESMSDPGELTWAFDRLNKGRFILGSPEECLEQIKEYQSRLGVEY
                     ILPRFDWTPGLPQEEILSSMRLFGEKVIQKL"
BASE COUNT          658 a          791 c          735 g          691 t
ORIGIN      
        1 caagaatgaa ggtaagaatg gagaagcgac tccgaaagcc gcatacatgg cggtatagag
       61 caggatgaat cttgtcaatg ggttggaatg cgaggaatcc aacatggctt ttccgtgacc
      121 ttagcagagt tctccgtatc ggacaccaat ggatgaatgc catgggacgg aacgcgctgc
      181 gatgactatc ttccgtgcac gaatcgataa acgataagga gggtttagga aagcgctcac
      241 cgcggagcct ggtagcagcc gcgctgagct agcttttatc ttgcttgtga tttcagtgag
      301 agcctactcg tcgcctcgca aaccactcga cgtcgagcgg ggctaatcgg accggttgaa
      361 cgaaacccta tcggcctcgc cggtcttgct cgtctgactt tcccccggtc tactttgatc
      421 atgttcttgg tcacgttcga acaaatcttt cttcgactgt tcagccgtcc cgtgttcctg
      481 ctgatcttct ttggcgcgat gattataaaa ttgaaaaaga accagcgcgc ccacggtcac
      541 tgccaacgcg ccgccgtact gcagtatttc caatgcctgc tttccgctaa tgaattcctc
      601 cagaaatctc accgccagga ttacgactac gacgctaata attttcgttt ccaggtcgtt
      661 aagagtttcc acgcctaagg aggttgcaat gttcagcggt gcaatgaaca ggctgtaaag
      721 tccgacaccg atcaaataga agaagaccgc cttcagcatg acgctgacga tctccagaaa
      781 ttcgaccgtt aagtccgtgc cttcgatctg gccgtgaagc aaagccgccc acgcttccca
      841 gatgcccatg accgccaaaa tcgttcccag cagaaatagt gagagcgcga ctaggagcac
      901 ggcgataacc gccagcagca cgacatatcg cgtgcgcccg ataacacgtc ccaaaggctc
      961 cattcgatcg ctgaagtctt tatcggggcc cgccgcgtaa ttcttggtca tgtgtcaacc
     1021 tttagtagct gtgcgttaat gtatcgaaag ggccaggctc agcctttgaa ctctgtcaag
     1081 gctaggtaag gttagcattg tttaatcgcc gtctcgcctt tacaactcaa cggtttgcca
     1141 tgatgcggtg taaacgaggc tggtaaccgg ccggtcggga aaactctctt cccgaaccgg
     1201 cgcttcgatt acattcaact taacttcgcg gcggcgatct caaattgagc agcttcgccg
     1261 gggtgatttt gtcttttgcc gaataccagg aggcgctgta cgctgactcg aagcgactat
     1321 ttacctccaa ttgcttgaat gaaggaacgg tcaatcagtt catcgagcga aatctttctc
     1381 ttgatccgcc cggttgcggc ttcctctgcg ataacctggg gaatcacctt ctcggcgatc
     1441 tgaccattga tcgggaagga atcgcggtat tcgtcatacc ctgccggagc gtaggccgga
     1501 tccattttta cgtatttctg catgatcttt actgaatcgg ttttgttatc gcgcgtgtat
     1561 ttcacgccct tctggtatgc ctggagaaac ttggtgacct ggtctttttc tgttttgagc
     1621 caagactccc tggcggcaaa agtctcgtag ggccactccg gaaacacttc cttgagatca
     1681 agcaacttcg tgtagccttg tgatttggcg atttcggtca cggggaacca gacaatggac
     1741 gcatccaccg ccttggccgt caacgccgca taacgggccg gcgtgctgcc gatttgcagg
     1801 atagtcacgt ccttggtgtc caacccgaaa tgacgcaagg tggcgcgctt cagaaagtcg
     1861 gtcaacgaac cgaaacggct gatggcgaac cgtttgccct tggcttcctt catcgatttg
     1921 aaatcgggct gggaataaag ctggaacggc atgagattac aaattcccca aaagtttttg
     1981 atatccattc ccgcggcgcg cccgccgacg gtttcgacga aggcgctccc catgatctgg
     2041 atgtcgccgc cggcaagagc ctgtagattg gtcgaaccgg cattgaacat gaccatgaga
     2101 acttctaatc cggcgtcttt gaaaaatccc ttttcctgag ccacgtacac cggcaagaat
     2161 gccacgttta ccgatgacag gccgacaacg attttggccg agctcagctg cggcagacta
     2221 acaatcaaca tcagcgccgc aaacgcgaca aatgccagta tagttcgttt catatgtgtc
     2281 ctccttggat ccggttgttt acagtttctg aatcaccttt tcgccgaaca gtcgcatcga
     2341 acttaaaatc tcctcctggg gtaagcccgg cgtccagtca aaacgcggca aaatgtactc
     2401 gacgcccaga cggctctgat actctttgat ctgctcaagg cactcctcag gggaaccaag
     2461 aatgaagcgg cccttgttta gacgatcgaa agcccaggtc aattcgccgg gatcgctcat
     2521 ggactcgttc tgcttccatt tgacgtagat cgtccgatag agctgttcca aacagggacg
     2581 cgccttttcc gtcgcttctt ttctggtctt agcgacaaac gtttcacgaa tgatcggaat
     2641 gtcaggcaaa cccggcttcc ctgcagcgct ccacgttcga cggcagagag ccacctggcg
     2701 ttgcaactca tcgatggcgg aatgcggccc gatcatccag ccgtcgccga tggtggcggc
     2761 acgagcaacc gcggcatcgg aattggccgc gatccaaatc ggcggccgcg gcttttgcaa
     2821 cggtttggga tcgacgctga cattcttgag cttaaatgct gtgccgtcat aatgc
//
LOCUS       DASUTG010000038         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1932718, whole genome shotgun sequence.
ACCESSION   DASUTG010000038 DASUTG010000000
VERSION     DASUTG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932718"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..742
                     /locus_tag="VF089_00800"
     CDS             23..742
                     /locus_tag="VF089_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:491..493,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX6172521.1"
                     /translation="MLVAKLNGTPFESEMLPVSFEPTPMPSAIKDLSRARVMLITDGG
                     LVPKGNPDKIQGTAATRWGSYSIEGRDDLRGEEYEISHGGYDARFVQEDPDRLVPLDA
                     LREMEKRGVIGKLHDEFISTSGRVNPLSNTRRLGREMAQKIKRDGVDAVILTSTUGTS
                     TRSGAAITTELEKAGIPVVQITSALPIAKMIGSNRVVLGNGIVHVTGDAKLSPDDEKD
                     LRRRLVQKALDALQSDETKSN"
     gene            898..1761
                     /locus_tag="VF089_00805"
     CDS             898..1761
                     /locus_tag="VF089_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6172522.1"
                     /translation="MEKTTNEKRKTLRALLQGPGCSIAPSCNDGIQARLVEWLGFPLV
                     HISGSGQHRALGFADAGMLTLTEMINRAREIVDAVELPVVSDGETGYGNAVNVVRTVR
                     EFERAGVAAIHIEDQMTPKRAGHEGFDVGLVPKAEFVAKIKAALDTRRDENLVIIARS
                     EAKDSLQERLDRVGACCDAGADAVWLSARTEDEIKAFAKLGKPLVGVPPRQLMTIDRY
                     GELGGRVGCIPTVLQVAALHGMRQCLEELKRSGTEARYFKETPGIEETRKWYTNLGNQ
                     ELKELEAKYGY"
     gene            1809..3164
                     /locus_tag="VF089_00810"
     CDS             1809..3164
                     /locus_tag="VF089_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6172523.1"
                     /translation="MDSITQAITEYASTSAFEHLPEEALHAAKQRLIDSLGCAVAAHD
                     CEPAQIGRRLARGQTPGKYPGRVLYFGDRLPVENAAFINTSMIRNLDFNDRYPGGHPS
                     DCLGALLAIAGAMDIDGKRFLTSMIVAYEIFARLSDATTLSRRGWDQGYAIGIATAAG
                     TCNILKIAPAATAHAVGIAAASNLPLRVTRSGELTPWKNAATAYATRNGVFAALLAAE
                     GMSGPGNAFDGRNGLFEKVTGPFELAPLPNCGGAHLTPRVQLKYWPIETNGQPAVWAA
                     LELREKARIDELKEIEVFTSKFTWFEIGSEPEKWNPQTRETADHSLPYIFARTLVDGP
                     IKITSFDDEAVRDPGLRPLMAKIKVIPDDHIEALLPQKTFMRVVATTLDERRHVVEIS
                     NPLGHSDNPMQDQHIEEKFTSLVEPALGKARCAAALDAWWRLDVVTGISAAMQLLDVN
                     R"
     gene            3299..>4544
                     /locus_tag="VF089_00815"
     CDS             3299..>4544
                     /locus_tag="VF089_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6172524.1"
                     /translation="MTADQNTFASVSEGLKTTAAAAAFIEQVTAGDLPSEAVRIGSRC
                     VLDGMGLYVAGSEEHSVKLLMDEAEHVGGRGEALLLGRGKIKVPAPTAARVLGTAGHA
                     HDWDDSQVSSDPAHVYGLLTHPTIPPLTSALVMSQRLGNISGQTFMLAFLTGFEVECK
                     ISEWMLPQHYLRGLHSSGTVGTFGAYATAAKLLGLKGENLRSGFGIAASFAAGIRCNF
                     GTMTKPLHVGRAAENGVTAALLAARGFTADLDALDGPWGFYAVHGGGVSAEKITQGFG
                     KTWTIVEPGVSIKPYPCGVLTHPTIDLMLKLVSEHNVKADDIAALKVYAGTNILKPIR
                     YPIAANHLQAKFSLPAALAMIALARKAGKREFSDEFVGSPAMQAMQRRISTEIDPEIE
                     KLGFDKMRSRIRIDLKDGRKIEG"
BASE COUNT          998 a         1258 c         1383 g          905 t
ORIGIN      
        1 cgctgcgcag cgggtggtgg acatgctggt ggcgaaactc aatggcactc cgtttgagtc
       61 cgaaatgctg cctgtttctt tcgaaccgac tccgatgccc tcggcgatca aagatctttc
      121 ccgggcccgg gtgatgctca ttaccgatgg cgggctcgtg cccaagggca acccggacaa
      181 aatccagggc acggccgcga cccgctgggg ctcttatagc atagaaggac gagatgatct
      241 gcgcggcgag gaatatgaaa tctcgcacgg cggttacgac gcccggtttg tgcaggaaga
      301 tcccgatcgc ctggtgccgc tggatgctct acgcgaaatg gaaaagcgcg gcgtcatcgg
      361 caagctgcac gacgaattta tttcgacttc gggcagggtc aatccgcttt cgaatacccg
      421 gcgcctgggc cgcgaaatgg cccagaagat taagagggat ggcgtcgacg ccgtcattct
      481 aacttcgact tgagggacga gcactcgcag cggcgctgcg attacaacgg agttggagaa
      541 ggccggcatc cccgtggtgc aaattacttc cgccctgccg attgccaaaa tgatcggatc
      601 gaaccgagtg gtgttgggta acggcatcgt gcatgtcacc ggcgatgcga aactgtcgcc
      661 ggacgatgaa aaagatttgc gccgcaggct ggtgcaaaag gctctcgacg ccttgcaatc
      721 cgacgaaacc aaaagtaatt aaccaaagct ctcgggcata gtcatccagt tcttactact
      781 ttgctattcc tggcatttga cccgcttcct cgtcttatga cacgatcggc cgcgaataat
      841 tggcctctct gcgaagaggg tgctgagaaa cttttaatct gaaagaaggt aaagtacatg
      901 gagaaaacaa cgaatgagaa acgtaagacg ttgcgcgcat tgctgcaggg accgggatgc
      961 tcgattgcgc cgagctgtaa cgatggcatt caggcgcggc tggtggagtg gctgggattt
     1021 ccactggtgc atatttccgg cagcggccaa catcgcgccc tcggttttgc cgacgccgga
     1081 atgcttacgc tcaccgagat gattaatcgc gcgcgtgaga tcgtcgacgc cgttgagctg
     1141 ccggtcgtca gcgatggcga gaccggttac ggcaatgcgg taaacgttgt gcgcacagtg
     1201 cgggaattcg agcgggccgg tgttgccgca atacacatcg aagaccagat gacaccgaaa
     1261 cgggccggtc atgaaggttt cgacgttggc ttggttccca aagctgagtt tgtcgccaag
     1321 atcaaggcgg cactggacac acgtcgcgat gaaaaccttg tcattatcgc gcgctccgag
     1381 gccaaggatt cgttgcagga gcggctcgac cgtgtcggcg cctgttgcga cgcgggagcc
     1441 gacgcggtct ggttgagcgc gcgcacggag gacgagatca aggccttcgc taaacttggc
     1501 aagccgctgg ttggggttcc gccgcgccag ctaatgacca tagatcgcta tggcgagctc
     1561 ggcggccggg tcggctgcat cccgacggtt ttacaggtcg ccgcgctgca cggcatgcgc
     1621 caatgtctcg aagagctgaa gcgtagcggc acggaagcac gctatttcaa ggaaactccg
     1681 ggtatcgaag agacccggaa gtggtacacc aatcttggca accaggaact taaagagctc
     1741 gaagcaaagt atggttattg aggagctgtg tcccggtgta aaaatcatca ctgaaccgga
     1801 agcatcccgt ggatagcatt acacaggcga tcactgagta cgcctccacc tcggcgttcg
     1861 agcacctccc tgaagaagcg ctgcacgccg ccaagcagcg gctgatcgac tcactcggtt
     1921 gtgctgtagc cgctcacgat tgcgagccgg cgcaaatcgg caggcgattg gcgcgcggtc
     1981 aaacgccggg gaaatatccc ggcagagtgc tctattttgg agaccggctg ccggtggaaa
     2041 acgcggcatt tatcaataca tcgatgatcc gcaatctcga ttttaacgat cgctatccgg
     2101 gcggacaccc gagcgattgt ctgggcgcgc tgctggcaat cgccggggcc atggacatcg
     2161 acggcaagcg ctttctcacg agcatgatcg tcgcttacga gatttttgcc cgattgagcg
     2221 acgccaccac cctgagccgc cgcggatggg atcaaggcta cgccatcgga atcgcgactg
     2281 cggcgggaac ttgcaacata ttaaagatcg cgcctgccgc gacggcccat gccgttggaa
     2341 tcgccgcggc ttccaacctg ccattgcggg tgacccgatc aggcgagctc actccgtgga
     2401 agaacgcggc caccgcctat gcgacccgca acggcgtgtt tgccgcgttg ctcgccgccg
     2461 aagggatgag cggaccgggc aatgccttcg acggacgcaa tggtctcttt gagaaagtaa
     2521 ccggaccgtt cgagctcgcg ccgttgccaa attgtggcgg cgcgcatttg acgcccagag
     2581 ttcaattgaa atactggccc atcgaaacca atggccagcc cgcggtttgg gcggcactgg
     2641 agctgcgtga aaaagcgcgg attgacgagc tcaaagagat cgaggttttt acctcgaagt
     2701 tcacctggtt tgagatcggc agcgagccgg aaaaatggaa cccgcaaaca cgtgaaacgg
     2761 cggatcacag tctgccctac atattcgcca ggactctagt ggacggcccg atcaagatta
     2821 catcttttga cgatgaagcg gtgcgcgatc ccggattgcg accgctcatg gctaagatca
     2881 aggttatacc ggatgatcac atcgaggccc tgctgccgca gaagaccttc atgcgggttg
     2941 tcgcaacgac tttggacgag cgacggcatg tagtcgaaat ttccaatcca ctgggtcact
     3001 cggacaaccc gatgcaagac caacacatcg aagaaaaatt tacttccttg gtcgagcccg
     3061 ccctgggcaa ggcacgttgc gccgcggccc tcgacgcctg gtggcgtctc gatgtagtga
     3121 ccggaataag tgccgcaatg cagttgctcg atgttaaccg ctaaggcgca ccggacagcg
     3181 cttcaaagca acgtgctggg aaccgcgccg cagtgcccgg ttttacgttc aacctgaccg
     3241 tggagtctta gcggcaaata caacctgagt gtcaaggctt cggagccaga ggagattcat
     3301 gacggcggat caaaacactt ttgcgtcagt ttccgagggc ctgaagacga cggctgccgc
     3361 cgccgcattc attgagcagg tgactgccgg agatctgccg agcgaggcgg tgcgcatcgg
     3421 cagccggtgt gtcctcgacg gtatgggtct ctatgtggct ggctcggaag agcattcagt
     3481 taagcttctc atggatgaag ccgaacatgt cggcggtcgc ggggaagcgc tgttgttggg
     3541 gcgtggcaag atcaaggtgc cggcgccgac ggctgcccgt gtcctcggaa ccgcggggca
     3601 tgcgcatgac tgggacgaca gtcaggtaag ctcggatcct gcccatgtgt acgggctact
     3661 cactcacccg acaattccgc cgctgaccag cgcgttggtg atgagccagc ggttgggcaa
     3721 tataagcggc caaaccttca tgctcgcgtt tcttaccgga ttcgaagtcg agtgcaaaat
     3781 ctcggagtgg atgttgccgc agcattattt gagaggttta cactccagcg gcaccgtcgg
     3841 aaccttcggc gcctatgcga ccgcggcaaa gctcctgggt ttgaaaggcg agaatctgcg
     3901 cagcggcttt ggcatcgccg ccagtttcgc tgcgggcatt cgttgcaatt tcggcaccat
     3961 gaccaaaccg ctgcacgtgg gacgggccgc ggaaaacggt gtgaccgcag ccttgcttgc
     4021 ggcgcgcggc ttcaccgccg acctcgatgc tctggacggc ccgtggggct tttatgcggt
     4081 gcacggagga ggcgtcagcg ctgaaaaaat tacacagggt ttcggcaaga cctggacgat
     4141 cgttgaaccc ggagtgtcca tcaaacctta tccctgcggt gtgttgactc atccgacgat
     4201 cgatttgatg ttgaagctcg ttagcgagca caacgtaaaa gccgatgata ttgcagcgct
     4261 caaggtttat gccggcacaa atattttaaa gcccatccgg tacccgatcg cggccaatca
     4321 cctgcaagct aagttctcct tgccggccgc tctggcgatg atcgccttag cgcgtaaagc
     4381 cggcaagcgc gaattttccg atgagttcgt gggttctccg gccatgcagg cgatgcagcg
     4441 gcggatatct accgagattg atccggagat cgaaaagcta ggattcgaca agatgcgctc
     4501 gcgcatcagg atcgatctca aagatggacg taagatcgaa ggct
//
LOCUS       DASUTG010000039         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_153824, whole genome shotgun sequence.
ACCESSION   DASUTG010000039 DASUTG010000000
VERSION     DASUTG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153824"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..686)
                     /locus_tag="VF089_00820"
     CDS             complement(48..686)
                     /locus_tag="VF089_00820"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX6172525.1"
                     /translation="MKIAILIYDGFTALDAVGPYEVLSCLPGAKVHFVSTELGPKRAH
                     TNFLSVMADYTLNDISDPEIIVVSGGTKGTMVAAKDPRILSWLRKAHETSKWTTSVCT
                     GSLILGAAGILKGLQATTHWYARDVLEKFGAQYLNERVVRQGKIITAAGVSSGIDMAL
                     HLAQEVSGPEMAKVIQLMIEYDPKPPFDSGSIAKAEPSIFELAKHAAQDAFK"
     gene            complement(1060..1497)
                     /locus_tag="VF089_00825"
     CDS             complement(1060..1497)
                     /locus_tag="VF089_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6172526.1"
                     /translation="MRLLTIDSSVFVSAARQTETGNAESTRFLAWVRESRPRLFLPTL
                     LMAEVAAALSRTGSAAGLAQQYALAIGQLPDTILVALDEGLAQRAAALGAQHKLRGAD
                     SVFLASAALFAAELVTLDREQLQRGSSIVQTLSPAEFLRTAKS"
     gene            complement(1499..1822)
                     /locus_tag="VF089_00830"
     CDS             complement(1499..1822)
                     /locus_tag="VF089_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172527.1"
                     /translation="MSKYRSRTEQSRKVKPKLGRPSIGIRELKAKASAIIDDVKGRRV
                     SYAVTKRGKVEALIVPSDAGERLLNHRNGDNAWDAWQTVADQLLKESGKKKRSAIAEL
                     ERMRR"
BASE COUNT          473 a          590 c          526 g          500 t
ORIGIN      
        1 aggtaatcgg cttgtcctat gcaaatcgca aaagatgcgg taactgttca cttgaacgca
       61 tcctgcgcgg cgtgtttcgc taattcaaaa attgaaggct ctgctttcgc gatggatcca
      121 gaatcgaaag gcggttttgg atcgtattcg atcatcaatt gaatgacctt cgccatctcc
      181 ggtcctgaga cctcctgcgc aaggtgaaga gccatatcaa tgcctgagga aacgccggcc
      241 gcggtgatga tctttccttg ccgaacaact cgctcattca ggtactgagc tccgaacttc
      301 tccagaacat cgcgggcata ccaatgcgtc gttgcctgca gcccttttaa aatccctgcc
      361 gcgccgagta ttagtgatcc ggtgcagacg gaggtagtcc acttcgaggt ttcgtgtgct
      421 tttcgaagcc aggagagtat gcgcggatct ttggccgcga ccatcgttcc ctttgttccc
      481 cccgaaacca caatgatttc aggatccgag atatcattca gcgtgtaatc cgccataaca
      541 gaaagaaagt tcgtatgcgc tcgcttcgga ccgagttccg tagaaacaaa atgaaccttt
      601 gctcccggta gacaactcag gacttcgtaa ggaccgaccg catcgagcgc ggtgaaacca
      661 tcgtagatca gaatcgcaat cttcatgaat cctccttacg aaaaccattc tactgtaaca
      721 gacgatactc gggattggat tgtcgaaaaa ttccaactgg gcaagatcaa ctcagcgctg
      781 ctcttcgctg gcagcggcga tgatccggaa tgacgcgctc cagattattg gcgacggatg
      841 tcgcgtcagt catgatgcta tgctccgttt ccaacagcgc gtgcatcttc taattgcttc
      901 aataccgatc gtgccggcga cgagttccgg atgctcgatg atgtcggtgg cgtggctggc
      961 aactcccgtg atcaggatct caccgacggc ggagtttcac gttgcgccaa ctacggcctt
     1021 cccgatatct gaatccgtcc caacgaattc cggcaatatt cacgacttag ctgttctgag
     1081 aaactcagcc ggagaaagcg tctggactat cgatgaacca cgttgcagtt gctcgcgatc
     1141 gagggtgacc aactcggcag caaacaacgc tgccgatgcc aaaaagacgg aatccgcacc
     1201 acggagcttg tgctgtgcgc cgagcgctgc cgcacgctga gccaaacctt catctaaagc
     1261 caccagaatt gtatccggta gctgcccgat agccagcgca tattgctgtg ctaggccggc
     1321 cgcactgccc gttcggctca gcgccgccgc gacttcagcc attagcaacg tcggcaagaa
     1381 caaacgcggg cgcgactctc gcacccacgc taagaatcta gtgctctccg cgttgccggt
     1441 ctcggtttga cgtgctgcgc tgacaaatac gctcgaatcg attgtaagca accgcacatt
     1501 accgccgcat ccgctcaagc tcagcgattg ccgatcgctt tttcttgccg gactccttga
     1561 gcaactgatc tgctaccgtc tgccaagcat cccaggcgtt gtcaccgtta cgatgattca
     1621 acagccgttc gccggcatcg ctgggtacaa tcagggcctc cacctttcca cgctttgtca
     1681 cggcgtagga aacgcgccga ccttttacat cgtcgattat tgctgacgct ttggccttga
     1741 gttcacgaat tccaatcgac ggtctgccta gcttaggctt gactttccgg gactgttccg
     1801 ttcgtgaacg atatttgctc atgtggtcac cttttagcag tatgaaacca caatggatcc
     1861 attctggcaa gtgatcagga tgaggaaaaa aggggtttcg gcatagcatt gatccgcata
     1921 cggtgctgtc gggaaggaga tcaaggacga gatttcttct agttttgtct tgccctgttt
     1981 ggtttacccg cagaggcatc ttttgaactc aactccgcaa cttcttgcgc gcaatcgccg
     2041 cgccgtttga atgggacctt cgggccgttc aagggatcaa cgttcaaag
//
LOCUS       DASUTG010000040         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1487893, whole genome shotgun sequence.
ACCESSION   DASUTG010000040 DASUTG010000000
VERSION     DASUTG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487893"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VF089_00835"
     CDS             complement(<1..1235)
                     /locus_tag="VF089_00835"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6172528.1"
                     /translation="MTINNFERLRELNQKAEAGGGELRARRQHEQGKLLARERLNILL
                     DPSSFVELDRLRTHGCTDFEMQEQKFLGDAVVTGYGAVDGRLVYVYSQDFTVFGGSLS
                     GVVAEKICKVMDLAIKNGAPIIGINDSGGARIQEGVLSLAGYGEIFLRNVKASGVIPQ
                     ISAIMGPCAGGAVYSPAITDFVYMVKSNSYMFITGPDVIKTVTHEEVTKDELGGAETH
                     SAKSGVAQFTANGEKECLLMIRELIAFLPSNNQEDPPFHPTNDDPLRRDKKLRDLVPD
                     NPNRPYDMKELIGAVVDEGYFYEVQRDFAPNLLIGFARLGGRPVGIVANQPAALAGCL
                     DINASVKGARFIRFCDCFNIPVITFVDVPGFLPGTSQEYEGIIRHGAKLLYAYAEATI
                     PKITIVTRKAYGGGYIVMS"
     gene            1419..2615
                     /locus_tag="VF089_00840"
     CDS             1419..2615
                     /locus_tag="VF089_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6172529.1"
                     /translation="MLRIKSNRTRRQPTGSSNTAARSLKADDALLLVAASEGDSNMLY
                     AVGFFVPDPFIFFQHKKKKYVVMSDLELDRAKRQATVDQVLSLSDYQRKLRMAGSPTT
                     MIGILELLFRERGIRSLTVPANFSALLADQLRHQGFHLEIRRDPFFPEREIKRAQEVR
                     HITESLRVATLGLDAGIRMLRKTKARPDGYLYLNGSRLTSETLKTAVNTTIMAQGWLP
                     SHTIISSGNQCVDPHHEGTGPIKANTSIIFDIFPRSQKNGYFGDLSRTVVRGRASDKL
                     KKIYATVQAGQKIGFDLIRDGLNGREVHQKILDLFAREGFPTGQIKGRMQGFFHGTGH
                     GLGLDIHEAPRIAPVDATLKSGHVVTVEPGLYYLGIGGVRLEDVVLVTDKGNKNLTDC
                     PQFLEI"
     gene            2669..3424
                     /locus_tag="VF089_00845"
     CDS             2669..3424
                     /locus_tag="VF089_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6172530.1"
                     /translation="MEEAKQIAEWLDALEQPDKQMLRAAVDGLIAVLPDSPETGKNLQ
                     DLLRNSQRKNRWPIAYILAHLPQPPAEALEILFDALDNRDPDVRWAVILLLTRLTEAQ
                     STVMQLMTSLFATGTATQRRMVIYYLKELKRRNEAVRQALLAALNDTDATVRVAAVIS
                     LKEQDIDSNGKTILLDLLMRDPDVRVQHAAAITLASLGSPSEDFLAALDNATKTAQGP
                     LKKAALAAQRILQEKKEVRPIRRLTSPVRRTSS"
BASE COUNT          869 a          950 c          944 g          812 t
ORIGIN      
        1 ctcgacatga cgatgtaacc gccgccgtat gctttgcgcg tgacgatagt aatcttggga
       61 atggtcgctt cagcgtaggc gtagagcagt ttcgcgccgt ggcggatgat gccttcgtat
      121 tcctgcgaag tgcccggcaa aaaccccggc acgtcgacga aagttatgac cgggatattg
      181 aaacagtcgc aaaaacggat gaagcgcgcg cctttgaccg aagcgttgat gtccaagcat
      241 ccagccaggg cggccggctg attggcgacg ataccaacag gacggccgcc tagacgagca
      301 aacccgatga gcagattcgg cgcgaagtct cgctgcactt cgtaaaaata gccctcgtcg
      361 acaactgcgc cgatcagttc tttcatatca taaggacgat tggggttgtc cggaaccaag
      421 tcgcgcaact ttttatcccg tcgcaacgga tcatcattcg tcggatgaaa cggaggatct
      481 tcttgattgt tactgggcag gaacgcaatc agctcacgga tcatcagtaa gcattctttc
      541 tcaccgttcg cagtgaattg tgctacgcca ctcttggcgc tgtgggtttc agctccgccc
      601 aattcatctt tagtgacttc ttcgtgagtc accgtcttaa ttacatcagg cccggtgatg
      661 aacatgtagc tgttggattt caccatgtag acgaaatcgg taatagccgg agaatagacc
      721 gctcctccgg cgcaaggacc catgatggct gagatctgcg ggatcacgcc ggatgctttc
      781 acgttgcgca agaaaatctc accgtaaccc gcaaggctca gcacgccttc ttggatacgg
      841 gcgccccctg aatcgttgat gccgatgatg ggtgcgccgt ttttgatcgc caggtccatt
      901 accttgcaaa ttttttccgc tacgacaccg ctaaggctgc cgccaaacac ggtgaaatcc
      961 tgggaataga cataaaccag ccggccatca acggcgccat agccggttac cacagcgtcc
     1021 ccaagaaact tctgttcctg catttcgaaa tcagtgcagc cgtgggtacg taaacgatcc
     1081 agttcaacga agctactggg atcgagaaga atgttcagcc gttcacgggc cagaagtttt
     1141 ccttgttcat gctggcgccg ggcccgtaat tcgcctccgc cagcctcggc cttttgattc
     1201 aactcacgaa gtctctcgaa attattgatg gtcatgtaga aacgttcctt agccgcgcaa
     1261 agtcagcggg tggcgggggt cacgagttgg agcataataa caaaagcgcc tagtgtgtca
     1321 acagaatttg gctttcggac gcttgtcttc attgaagttg gatcgtgaac ttgttagcct
     1381 agttctcttc ccacaaattt ccactaacgc tggagaaaat gcttcgaatc aaatccaatc
     1441 ggacccgccg tcagcctacc ggatcctcga acactgccgc tcgatcgttg aaagcagatg
     1501 atgcactgct tcttgttgcc gctagcgaag gcgactccaa catgctctat gctgtgggat
     1561 tttttgtccc ggatccattt attttttttc agcacaagaa aaagaaatac gtggtgatga
     1621 gcgatctcga gcttgaccgc gcaaagcgtc aggcgacggt ggaccaggtg ctttcgctct
     1681 ccgattacca acgaaagctg agaatggcgg gcagccctac gacgatgatc gggattctcg
     1741 aactgctgtt ccgcgagcgc ggcatccgat cattaacggt gccggcgaac ttttcagctc
     1801 ttttggcgga tcaactacga catcaaggct tccatcttga aattaggcga gacccctttt
     1861 tccctgagcg tgaaatcaaa cgcgcgcaag aagtgcggca cataaccgag tcgctgcgtg
     1921 tggccacatt ggggctcgat gccggcatca ggatgctcag gaaaaccaaa gcgcgacctg
     1981 acgggtattt atatctaaac ggctcgaggc ttacatccga aacactgaaa accgcggtga
     2041 acaccacgat catggctcaa ggttggctgc ccagccacac gatcatttca tccggcaatc
     2101 agtgcgtcga tcctcatcat gaaggaaccg gcccaatcaa ggcgaacacc tcgattattt
     2161 ttgatatctt cccacgctcg caaaagaacg gttatttcgg agatctcagc cggactgtgg
     2221 tgcgcggccg ggcatcagac aagctcaaga agatttatgc gacagtgcag gccggacaaa
     2281 agatcggctt tgatctgatc cgcgatggcc tcaatggcag agaggtccac caaaagatac
     2341 tcgatctgtt tgcccgtgaa ggttttccca ccggacaaat caaaggtcgc atgcagggat
     2401 tcttccatgg caccggccat ggacttggtc tggatatcca tgaggcgccg cgaatcgcgc
     2461 cggtcgatgc cacgctcaag agcggacatg tggtgacggt agaacccgga ctttattacc
     2521 tcggcattgg cggcgtacga ttggaggatg tggtgctggt caccgacaag ggcaataaga
     2581 atctcaccga ctgtcctcag tttctggaaa tctgatgggc ctctcagctc accagggacc
     2641 cgcgcgagct gtaatcagga ttgatagagt ggaagaagcg aagcagatcg ctgaatggct
     2701 tgacgcgctg gagcagcccg acaagcagat gctccgcgca gcggtggatg gtttgatcgc
     2761 ggtgttgccg gactcacctg aaacggggaa gaatctgcag gacctgcttc gcaattcaca
     2821 gcgaaaaaat cgctggccta tcgcatatat tctcgcccac ttgcctcaac cgcctgcgga
     2881 agcactggag atcctgttcg atgctctcga caatcgtgac ccggacgtgc gttgggccgt
     2941 tatcttgctg ttgacgcggt tgacggaggc ccaatctaca gtgatgcaac taatgacatc
     3001 tctctttgcc actggtacag caacccaacg gcgcatggtc atctattatt tgaaagagtt
     3061 gaaacggcgg aacgaagctg tccgtcaggc gctgttagcg gcgctgaacg atactgatgc
     3121 cacagttaga gttgctgctg tgatcagctt gaaagaacag gacatagatt cgaacggtaa
     3181 aacaatttta ttggacttgc tgatgcgcga tcctgatgtt cgggtacagc atgcggcggc
     3241 gataacgctg gcaagcttag gatcaccgtc cgaagacttt ttagcggctc tggataatgc
     3301 cacgaaaact gcgcaggggc cgctgaagaa ggctgctcta gcagcacagc gtatccttca
     3361 agaaaaaaaa gaggtccgtc ccatacggcg actaacgtca ccggtgagac ggacctcaag
     3421 ttaacttcgg tagactgagg gggattgcta ggcttcaagc tagaatcccc gtcaaagatc
     3481 cacttgaagc ctaagtctca gtcacctcgc agacaaaaca attatcatca atcatgtctg
     3541 gaccacctcc tttccctgct gacttttagt ttaag
//
LOCUS       DASUTG010000041        12722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1784624, whole genome shotgun sequence.
ACCESSION   DASUTG010000041 DASUTG010000000
VERSION     DASUTG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12722
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784624"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF089_00850"
     CDS             <1..324
                     /locus_tag="VF089_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172531.1"
                     /translation="DLVLRELRCLAAGEIKEDEVRRTRGQLVGNIMLGLESTDSWMSH
                     IARNELYFGKSITTDEICQRIRSVTRDEIIELAGALFRPETMAVSLLGDFKESFKIKN
                     FALGQ"
     gene            355..819
                     /gene="dut"
                     /locus_tag="VF089_00855"
     CDS             355..819
                     /gene="dut"
                     /locus_tag="VF089_00855"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX6172532.1"
                     /translation="MEEIQIAIKRVRPGGAPASLPSYMTEGAAGMDLCADLSHDLQLE
                     PLRRTLIPTGIAIALPLGFEAQIRPRSGLALKEGLTLINSPGTIDADYRGEIQLITIN
                     LGSDSIIIRPGQRIAQMIIQRVVRALWQEVAELPHSERQAGGFGHTDDIRKS"
     gene            885..2177
                     /gene="purB"
                     /locus_tag="VF089_00860"
     CDS             885..2177
                     /gene="purB"
                     /locus_tag="VF089_00860"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX6172533.1"
                     /translation="MIPRYTRPEMGRIWSEENSFQKWLDIEVLAAEALAQMGKVPKAA
                     ISRIRKNARFNVERIREIEREVKHEVIAFLSSVAESIGDDARFLHLGMTSSDVMDTAL
                     AIQLKEAAAILIEDVQSLMKVLRRQAYKYKSTVMIGRTHGVHAEPITFGLKFALWHQE
                     MDRNLVRLRKAVEDISVGQISGAVGTFAQISPKVEAYVCKMSGLKPALISNQIIQRDR
                     HAYFFATLAVIAGSLEKFAVEIRHLQRTEVQEAEEPFTPGQKGSSAMPHKRNPILSEN
                     VSGLARLMRSYALAALENVALWHERDISHSSVERVIAPDATIALDFMLRRMTYVLGEL
                     CVYPESMKRNLEKSGGAVFSEGVLLALVDKGVSRDAAYRMVQRHALKVARDGGELKHE
                     LLKDREIATYLSASEVEAVWGLKHHLANVDFIFRRVFR"
     gene            2204..2449
                     /gene="purS"
                     /locus_tag="VF089_00865"
     CDS             2204..2449
                     /gene="purS"
                     /locus_tag="VF089_00865"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015842490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX6172534.1"
                     /translation="MRVKIFVSLKNGVLDPQGKAVQQSLHTLGYKEVQDVRVGKFVEL
                     NLQTNSREAAEGRIREMCDKLLANPVIENFHYEITEG"
     gene            2589..3299
                     /gene="purQ"
                     /locus_tag="VF089_00870"
     CDS             2589..3299
                     /gene="purQ"
                     /locus_tag="VF089_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX6172535.1"
                     /translation="MRWGVIVFPGSCDDQDVFDSLKREMGQKVKFLWHKDDLVGHFDC
                     LVLPGGFSYGDYLRCGAMARFSPIMRGVEKFAQEGGLVLGICNGFQILCEAGLLPGAL
                     VRNVGLKFVCQQTWLRVEETDAPFTSQLKKGQLLRIPVKHGEGRYHADELTLERLHRH
                     RQVLLRYVNAKGKASPAANPNGSVDDIAGICNERRNVFGLMPHPELACNKLLGSDDGL
                     KIFSSIAAACDSKSAAHA"
     gene            3292..5520
                     /gene="purL"
                     /locus_tag="VF089_00875"
     CDS             3292..5520
                     /gene="purL"
                     /locus_tag="VF089_00875"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX6172536.1"
                     /translation="MLDQSASKQPQVTAELVASHGITPDEYQKIIQILRREPNYTELG
                     VFSVMWSEHCSYKSSRQYLKSLPTEGPHVLQGPGENAGVVDIGDGLAVAFKMESHNHP
                     SFIEPYQGAATGVGGILRDIFTMGARPIASLNSLRFGSIDHPRTPYLVSGVVAGIGGY
                     GNCIGVPTVGGEVYFDECYDSNIVVNAFTLGITKKKRIHTGVAKGRGNPVMYVGSRTG
                     RDGIHGATMASESFSEEKEQRRPTVQVGDPFTEKLLLEACLELMEHDYLIGIQDMGAA
                     GLTSSSTEMAGRGGAGIELDLSAVPLREQGMTPYEILLSESQERMLLVAKAGTEAEIK
                     RIFDKWDLEAVVVGRVTDDNEFRALFNGAQVARIPISALTKGAPDYHRPAERPKSQDD
                     LEQLDLSAIAEPRDLGATLKLMLASPNIASKEWVYRQYDCYVRSNTVVAPGADAAVVR
                     IKGTDKGLALTVDGNSRYCFLDPYVGGALAVAEAARNLACVGARPIGLTDCLNFGSPE
                     NPAVMWQFARVIQGMRDACVALKVPVVSGNVSFYNETDGVPIYPTPTIGMVGLLPKVD
                     RVITPWFKAAGDIVVLLGRTREELGGSEYLKFIHNIVRGTPPWIDLKLEQAIQNCCIQ
                     AIEGGILRSAHDISDGGLAVALAECCIGGPDKPLGVRTEMREMIRGDALLFSESQSRI
                     LVSMQEKDLSQLQEIASRFAVPLQVIGAVGGSRFSIQPILQLPVEELQSIWSSALSDR
                     LK"
     gene            5610..7007
                     /gene="purF"
                     /locus_tag="VF089_00880"
                     /pseudo
     CDS             5610..7007
                     /gene="purF"
                     /locus_tag="VF089_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
     gene            complement(7299..7574)
                     /locus_tag="VF089_00885"
     CDS             complement(7299..7574)
                     /locus_tag="VF089_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX6172537.1"
                     /translation="MATAKKKTRKPNAAFMKPVQPDAALSAVVGTKAIPRTEVTKKLW
                     AYIKKNGLQDKKKKTNINADDNLKVVFGGKKQVTMFEMTKLVSKHLK"
     gene            complement(7646..9379)
                     /locus_tag="VF089_00890"
     CDS             complement(7646..9379)
                     /locus_tag="VF089_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6172538.1"
                     /translation="MSVDPITLQVIQARLAGIVQEMQNSLFRTGYSTIIRESQDASCA
                     ILNRQGEVVAQHVVLPLHMGAFPACAEAILGTYELAEIQQGDAFITNHPYLGGSPHAP
                     DMGVFTPIFYNGEWVGFAANMAHKSDIGGTVPGSGSGSAREIFQEGLHLPPVKFMSRL
                     RPIQEIEAMIAANSRTAGVVLGDLRGQVGAARLGERRIAELMERYGKQTILDCTAVLS
                     ANTERRVRQVIETWPNGESEGESFVDHDGIDLSRPIRIHVNVTKTDDRIHFDFSGSSD
                     QTQGPANIRPPLVRAACVYCLVALVDRFLPINQGLARVVDAKFRPGSVVDPYFPAAVN
                     TYMPTALTVAEAVLRALAFFVPEKRISGGSGSAALVLGGRDAQTQRAYVHYEIFSGGT
                     GARWSKDGVSATAFHLSNCKTAPVEIIESEFPTRVERFEIIPDSGGAGRWRGGLGFAR
                     DYRILSDEVRFSMRTDKHAVEPFGTDNGLPGRRGSCIVNPETDKETRLPSRFGDQRLS
                     KGDLLRIERPGGGGLGDPLDRAAEQVLDDVRRGYVSVERAKSDYKVSISYSDSEPCID
                     SVKTQVLRGKS"
     gene            complement(9443..11176)
                     /locus_tag="VF089_00895"
     CDS             complement(9443..11176)
                     /locus_tag="VF089_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6172539.1"
                     /translation="MNVDPVTLQVIQARLAGIVQEMQNSLFRTGYSTIIRESQDASCA
                     ILNRQGEVVAQHVVLPLHMGAFPACAAGILKNYSAEEIHKGDAFITNHPYLGGSPHAP
                     DMAVLSPIFFHDEWVGFAANLAHKSDIGGPVPGSCWSQAREIYQEGLHVPPIKFVHNY
                     QTSKDIEAVLGANSRTPELVVGDLRGQVGAARLGENRFQEMMERYGKETILSSFEQLF
                     ALTEAKVRTEIASWPDGVGEGERFVDSDGIDLDTPVRLHVRMEKKGRELLFDFTGCAD
                     QTKGPANIRPTIVRAACCYLLTCLVDPALPINYGLAKVIDIKVREGSVVNPRFPAPVN
                     TYNPTVHALVESLLEASSQITPHKKVGDGCSSRSIIIGGRSNKTGKSYVQYEIFGGGS
                     GGRSGKDGVSGTNVNQSNAKIAPIEIIESEFSTRLRRFALIPDSGGPGTFRGGLGFVR
                     EYEVLDNDARFSLRSTKHTVAPKGIDGGGNGKTGHCTLNPGKPDERAIPSRFSDHVLH
                     PGDVFSLETPGGGGLGNPLQRDPQRVLSDVRNGYVTRAKAREVYRVAVDERDSDFILN
                     ESETRALRTQT"
     gene            complement(11289..>12722)
                     /locus_tag="VF089_00900"
     CDS             complement(11289..>12722)
                     /locus_tag="VF089_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6172540.1"
                     /translation="AYIQPVMANYLGRLENRMREMGITTPKLYIMQSNGGVSTFAGSA
                     KKPVATVLSGPAGGVIASMGTCERVGINNIITFDMGGTSCDVALIHKGNPVITTQGKI
                     NLRPIALPMLDIHTVSAGGGTIARLDAVGGLQVGPDSAGADPGPVCYDRGGENITITD
                     ANMVIGVLDPDHFLGGRMKLNKAKAQRLLEEKIAKPLTMDLMEAAHGVLDIINVKMEE
                     AIKAVSSQRGYDIRDFTLVAFGGGGPMHAGKMALDLGIPSVLIPLTPGVHSALGLLMS
                     DVKHDYVRSKLVGLDDLDLDEINPMFGQLIEQAKADLYGEGFRDSEIRMEPYLDLRYA
                     GQGYELTVPCPMPPLNKDDLALMRARFDTVHEQNSGHKAETEPVELVSLRLISLGMVP
                     QAKLSPGKVSGRTVEEARTGTRKVFFGKEHGMLKTQVYSRDALEPGHGLSGPAIVEQL
                     DTTTVIHPEQEVTVDDYRNLIMRSKQQ"
BASE COUNT         2797 a         3446 c         3583 g         2896 t
ORIGIN      
        1 gatctcgtgt tgagggaatt gagatgtctg gctgccggag agattaaaga agacgaggtt
       61 cgccgcaccc gggggcaatt ggtaggaaat atcatgctgg gtttggaatc cacggactca
      121 tggatgagtc atatcgctcg caatgagctt tacttcggca aatcgattac cacagatgag
      181 atctgtcaac gtattcgctc tgtcacccgg gatgaaatca tcgagctggc cggcgcctta
      241 ttccggccgg agacaatggc ggtttccctg ttgggtgatt tcaaggaaag tttcaaaatc
      301 aaaaacttcg ccctcggcca gtagtcatca cccgtcgatc ggagttcggc cgctgtggaa
      361 gagattcaga tagcgatcaa gcgagtgcgt cccggtggcg caccggcttc actgccatcc
      421 tatatgacag aaggggcggc gggcatggat ctatgcgccg atctttctca tgacctccaa
      481 ctggaacctc tacggcgaac gctgattccg accggcattg cgattgcgct gccgttgggc
      541 ttcgaggcgc aaattcgccc gcgcagcggg cttgctttga aagaaggact gaccctaatc
      601 aacagtcccg gaacgatcga cgcagactac cggggtgaaa tccagctcat taccatcaat
      661 ttgggttcag actcaatcat cattcggccg ggacagagga tcgcacaaat gattattcaa
      721 cgggttgtgc gcgcgctgtg gcaagaagtc gccgaattac ctcattccga gcgtcaggcg
      781 ggaggcttcg gccataccga cgacatccga aaaagttgaa cccccaatcg catttcacga
      841 aatacaccat cagccgatag ttaaactaca ggcttacagc gaacatgatc ccacgttata
      901 cacgaccgga aatgggtcgc atctggtcgg aggaaaatag ctttcaaaaa tggctcgaca
      961 tcgaagtcct ggccgctgag gctttggcgc aaatgggcaa agttccaaaa gctgctatca
     1021 gcaggatcag gaaaaacgcg cgtttcaatg tggagcgtat ccgcgagatc gagcgcgagg
     1081 tcaagcacga agtcattgca ttcttaagct ctgtggccga atccatcggc gatgatgcgc
     1141 gatttcttca tctcggcatg acctcctcgg acgtgatgga taccgccctg gcgattcaac
     1201 tcaaagaagc ggcggcaatt ttaatcgagg atgtgcagag tttgatgaag gtcctgcgcc
     1261 gccaggccta caagtacaag tcaacggtga tgatcggccg cactcatggc gtccatgccg
     1321 agccgattac attcgggtta aagtttgcgc tttggcatca agagatggat agaaaccttg
     1381 ttcggttgag gaaagccgtt gaggatatca gcgtcggtca gatttcagga gccgtcggca
     1441 ccttcgccca gatctctccc aaagtcgaag cttatgtttg taagatgagc ggacttaagc
     1501 ccgcgttgat ctccaatcag atcattcagc gcgaccgcca tgcgtacttc tttgccacat
     1561 tggcagtcat cgccggttcg ctggagaagt ttgctgtgga gattcgtcac ctgcagcgga
     1621 ccgaggtgca ggaggcggaa gaacccttta cgccagggca gaagggctca tcggccatgc
     1681 cgcacaaacg caacccgatt ctatcggaga acgtgtcagg attggcgcgg ctgatgcggt
     1741 cttatgcatt ggccgcgttg gaaaatgtgg cgctgtggca tgaacgggat atcagccact
     1801 cttcggtaga acgagtgatt gcgccggatg cgacgatcgc gctggatttc atgttgcggc
     1861 gcatgaccta cgtgctggga gagttatgtg tctaccctga gagcatgaaa cgcaacctgg
     1921 aaaaaagcgg cggagccgta ttctcggagg gcgtgcttct cgcattggtc gacaagggcg
     1981 tgtctcgaga tgcggcttat cgcatggttc aacgccatgc cttgaaagtc gccagagatg
     2041 gtggtgagct aaaacatgag ctgctaaaag accgggagat cgcgacttat ctctcggcga
     2101 gtgaggtgga agcggtgtgg ggtttaaagc atcatcttgc caatgtagac ttcatctttc
     2161 gcagagtatt ccgttaaatc aatagccgga gacgaaggtg ccaatgcggg tcaaaatatt
     2221 cgtgtcacta aagaacggcg tactcgatcc tcagggtaaa gccgtccagc agtcgctgca
     2281 tactttgggc tacaaagagg ttcaggatgt tagagtcggc aagttcgtcg aactcaattt
     2341 acaaactaat tcgcgcgagg cagcggaagg gcgtatccgt gagatgtgcg acaagctgct
     2401 ggctaacccg gtgatagaaa acttccatta tgaaataact gaaggctgat aatagtttta
     2461 tgcgccgagg gcctcaaagc tacaaacaca gatgatagcg tgcgttgggg tgtaattgtt
     2521 tttccaggtt cgtgtgacga tcaggatgtc ctcgacagcc tcaagggtag aaatagaggg
     2581 gtgatagcgt gcgttggggt gtaattgttt ttccaggttc gtgtgacgat caggatgtct
     2641 tcgacagcct caaacgagag atgggccaga aggtgaagtt tctctggcac aaggatgatc
     2701 tagtcggcca tttcgactgt ctagtgcttc caggcggctt ttcctatggc gattatctcc
     2761 gttgcggcgc gatggcgcgt ttttcgccaa tcatgcgcgg cgttgaaaag ttcgctcaag
     2821 aagggggcct ggttctaggc atttgcaatg gttttcagat cctttgtgaa gcgggactgc
     2881 tccccggcgc gctggtgcgc aatgtcggcc tcaagtttgt ttgtcaacaa acttggctgc
     2941 gggtggaaga gaccgacgcg ccctttacct cacagctcaa aaaagggcag ctccttagga
     3001 ttccggtcaa gcacggcgaa ggccgttatc acgccgatga attgacgtta gagcgattac
     3061 accggcatcg ccaggttctt ctacgctacg tcaatgccaa aggaaaagct tcgcccgctg
     3121 ccaaccccaa cggctcggta gatgacatcg ccggcatctg taatgagagg cgcaacgtgt
     3181 ttgggttgat gccccatccg gaacttgctt gcaacaagct cctcggcagc gatgatgggc
     3241 tgaagatttt ttcctcaatt gcggcagcct gcgattcaaa gagcgccgcg catgcttgat
     3301 cagagcgctt ctaagcagcc acaagtcact gccgagctgg ttgcgagcca cggaatcact
     3361 ccggatgagt accagaagat cattcagatt ctgcgacgag agcccaatta taccgagctc
     3421 ggcgtgttct cagttatgtg gtcggagcac tgtagctata aaagctcgcg ccaatactta
     3481 aagagtttgc ccactgaagg gccgcatgtt ctgcagggtc ccggtgaaaa cgccggggtc
     3541 gtggatatcg gcgacggttt ggcagtggca ttcaagatgg aaagccacaa ccatccatct
     3601 ttcattgaac cctatcaagg ggcggctacc ggcgttggcg gcattttgcg ggacattttt
     3661 accatgggcg cccggcccat cgcctcgttg aattcgttgc ggtttggcag catcgaccat
     3721 ccgcgcacgc cctatctggt ttcaggggtg gtggcaggca ttggcggtta cggcaactgc
     3781 atcggtgtgc ccaccgtcgg aggcgaggtt tacttcgacg aatgctatga ctccaacatt
     3841 gtggtcaacg cgttcacgtt aggaatcacc aagaagaagc gcattcacac cggggtcgcg
     3901 aagggccgtg gcaacccggt gatgtatgtc ggatcgagga ccggccgcga cggcatccac
     3961 ggagcgacga tggcctctga atcgttctct gaggagaaag agcagcgccg cccaaccgtg
     4021 caggtcggtg atcccttcac tgaaaagctt cttctggaag cctgtttgga attgatggag
     4081 cacgattatc tgatcggtat tcaagatatg ggcgccgctg gattgaccag ttcatccaca
     4141 gagatggctg gtcgcggcgg cgccgggatt gagctcgatt tgtcggcagt gcctttgcgc
     4201 gaacagggga tgacgccgta cgagatttta ctctccgagt ctcaggaacg gatgctactg
     4261 gtcgccaaag caggaacgga agcggagatc aagcgaattt ttgacaaatg ggatcttgag
     4321 gcggtggtcg tcggccgggt caccgatgac aacgagttcc gagctctgtt caacggtgct
     4381 caagttgcgc gcatcccgat cagcgcttta accaaaggag ccccggatta tcaccggccc
     4441 gccgagcggc ccaagagtca agacgatctc gagcagctgg atctgtcggc aatcgcagag
     4501 ccgagagatc tcggagcaac tttaaagctg atgcttgctt cgcccaatat cgcctccaag
     4561 gaatgggttt atcgtcaata cgattgctat gtgcgcagca ataccgttgt cgcccccggt
     4621 gccgacgcag cggtagttcg catcaagggg acggataaag ggcttgcgct gacagtcgac
     4681 ggcaacagtc gttattgttt tcttgatcct tatgttggcg gcgctcttgc cgtggcggag
     4741 gcggcccgca acttggcctg tgtcggcgcc cggccgatcg ggctcactga ttgcctgaac
     4801 tttggcagtc cggagaaccc ggctgtcatg tggcagtttg cgcgtgtcat ccaaggcatg
     4861 cgcgatgcct gtgttgcgct caaagtgccg gttgtgagcg gcaatgtaag tttctataat
     4921 gaaacagacg gagttcccat ttatccaaca ccaacgatcg ggatggttgg tctcctgcca
     4981 aaggttgacc gagtcatcac cccttggttc aaagctgctg gcgatatcgt ggtgctgcta
     5041 ggccgcacgc gtgaagaact tgggggcagc gaatacctca agttcatcca caatatagtt
     5101 aggggcacac cgccgtggat tgatctcaag ctggagcagg cgatccagaa ctgttgtatc
     5161 caagctatcg aaggaggcat ccttcgttcg gcccatgaca tatcggacgg tggcttggct
     5221 gtggcattgg ctgagtgttg tatcggcggt ccggataaac ctttgggagt tagaaccgag
     5281 atgcgggaaa tgatacgcgg cgacgcctta ctttttagtg aatcgcaatc gcgtattctg
     5341 gtatcgatgc aggagaagga tttaagccaa ctgcaagaaa ttgcctcccg gttcgcggtg
     5401 ccgctccagg tcatcggcgc agtcggcggt tcgcggtttt ccattcaacc gattctgcaa
     5461 ttgcccgtcg aggagctgca gtcgatttgg tcgtcagccc tgagcgatcg gttgaaataa
     5521 tcgcctcagc gactgcggct gcgaactgtt tagaagcacg gaattcggtt tcaagagtga
     5581 cggataaatt tcacgacgaa tgtgcggtga tgggcgtcta cggtcacccc gaagcggcga
     5641 acatggtcta cctcggtctt tacgctctgc agcaccgtgg tcaggagtcg tgcggcatcg
     5701 tgtcttcaga tggtaaggcg ctcatctcac acaggcaaat gggtctggtc gccgatgtct
     5761 tcaaggaaga tgtcatcaaa cgcttggaag gccaaagcgc cataggccat aatcgctact
     5821 ccactcaagg ccaaagtcat cttaaaaacg cgcagccctt tgtcgttgag tattcgcagg
     5881 gccccatcgc catcgcgcac aacggcaact tggttaatgg cgcgatcttg cgcgatgagc
     5941 tcgaacgctc aggctcgata ttccagtcga cttccgacac cgaagtgatc attcatttga
     6001 ttgccacctc caaagagatt tcgctcatgg gacggatcgt cgaagcgctc agccgcgtgc
     6061 ggggcgccta ttcgttgctc ttcatcacac ccgatcgaat gattgccgcc cgggattcgt
     6121 atgggtttcg cccattggta ttgggtaaat ttcgcgaggg acccaatagc ggagcctacg
     6181 tggtcgcctc ggaaacttgc gcgctggatc taatcgaagc cgactatgtg cgcgagatcg
     6241 aacccggcga gatcattacc ttcggtccgg acggcatgga atcgctaaag ccatttccgc
     6301 cggtgcccca ggcaaaatgt atctttgagt acatttactt tgcccgccca gacagcacgc
     6361 tgtacgggca caacgtttac aatgttcgga aggctatggg tcgccaactg gccaaggaaa
     6421 gcgctgtcaa tgcggatctg gtaacaccgg tgcccgactc cggggtaccc gctgccatcg
     6481 gctatgccga ggaatcaaaa atcccgcttg agttcggctt gattcgcaac cattatgtcg
     6541 gccgcacgtt tatcgagccg cagcaaacaa ttcgtcattt cggagttaaa attaaactca
     6601 atgcccagcg ggaagttctc aacggtaagc gcgtcattgt agttgatgat tccatcgtgc
     6661 gtggcacgac aagccgtaag attattcgca tgctgcgcga tgccggcgca aaagaagttc
     6721 acaggcgcat cagctcgccg gctaccacgg gtccgtgtta ctagggtatc gatacaccga
     6781 cccgaaccga actgattgca tcatccaatt cgctggaaga tatccgccgc tttatcgaag
     6841 ccgacagtct cgcttacctt agccgtgacg gtatgtatga ttacttcgac ggaaaccaac
     6901 agggcttctg cgacgcctgc tttagcggca actacccggt tcatttcgaa gacgaagggc
     6961 acacacgcca gcttcacctg ttcgaggcgc tgcagcatcg gtcataaccg cacgtacagc
     7021 gcgttgcggt agccatcggc gaatccgccg aattgcggct ttcgccggtc gaggtagctt
     7081 cggctaagct gtcaaatcaa acgacaaatg gaaggcctga tagaggtcgc ggcattggca
     7141 acgagcaggc tgcctggaac aggcagcaat agatgtcagc cgaaatcgtc gctgcagcgg
     7201 cgaaccacaa cattcaatcg gtgcatcaag accgatgatg agatcaccgg ccatgatgca
     7261 ttccgaaata tactcgtcca aaaataaaga gggactgttt acttgaggtg tttgctgacc
     7321 agtttggtca tctcaaacat ggtgacttgc ttcttgccac cgaaaacaac tttaagattg
     7381 tcgtcggcgt tgatgttggt ctttttcttt ttgtcttgga gcccgttctt tttgatatag
     7441 gcccaaagct ttttggttac ctcggtgcga gggatagctt ttgtcccgac aaccgcgctc
     7501 aacgctgcat ccggttgcac gggtttcata aaggctgcat tcggcttgcg ggtttttttc
     7561 tttgctgtcg ccatttttcc tccttcaagg ggcgcacata ttagggtgct tataccagag
     7621 gcccgcgatt tttgctactt attttttacg atttacctcg taaaacttgc gtcttaaccg
     7681 aatctataca cggctcgcta tcgctgtaac tgatcgacac cttgtagtcg gacttggcgc
     7741 gctcgactga aacgtagcct cggcgcacgt catccagtac ttgttcggct gcgcgatcca
     7801 agggatcacc gaggccgccg cccccgggac gctcgatgcg caaaagatct cctttgctga
     7861 gcctttgatc gccaaaacgt gatggcaacc gggtttcttt gtcggtctcg ggattgacga
     7921 tgcacgatcc tctgcggccg ggcaaaccgt tgtcggtgcc gaacggctca acagcatgtt
     7981 tgtcggtgcg cattgaaaag cgaacctcat cactgaggat ccggtagtcg cgcgcaaagc
     8041 ccaaaccacc gcgccatcgg ccggcgccgc ctgaatccgg tattatctca aaccgctcga
     8101 cgcgggtggg aaactccgat tcgatgattt cgacaggcgc ggtcttgcag ttgctcaagt
     8161 gaaatgccgt tgccgatacg ccgtctttgc tccatcttgc tccggtgccg ccgctaaaga
     8221 tctcatagtg aacataggcc cgctgggttt gagcatcgcg tccgcccagc accagcgcgg
     8281 cgcttcccga gccgcccgaa atccgcttct cgggaacgaa gaaagcaagt gcccgaagga
     8341 cggcttccgc caccgtcaac gcggtcggca tgtacgtgtt taccgctgcg ggaaaatagg
     8401 gatccacgac actgccgggg cgaaactttg cgtccacgac ccgcgcgaga ccctgattaa
     8461 tcggcaaaaa acggtccact agcgcaacca gacagtagac gcacgccgcg cgcaccagcg
     8521 gcggccgtat gttggcggga ccctgagttt gatcactgct gccgctgaaa tcaaagtgaa
     8581 tccgatcatc ggtcttcgtt acattcacgt gaatccgaat cgggcggctc aagtcgatgc
     8641 cgtcatgatc gacaaagctc tcgccttcgg attcgccgtt cggccatgtt tcgatgactt
     8701 ggcgaacacg cctttcggta tttgcagaga gcacggcagt gcagtctagt atggtttgtt
     8761 tgccgtaacg ctccatgagc tcggcgattc tgcgctcacc caaacgggct gcgcccactt
     8821 ggccgcgcag atcgccaaga accacgcccg cagtccggct gttggcggcg atcatggcct
     8881 cgatctcttg gataggccgc agccgcgaca taaacttgac cgggggtaga tgaaggcctt
     8941 cttgaaagat ttcacgcgcg ctgccggaac cgcttcccgg caccgtgcca ccgatgtcac
     9001 tcttgtgcgc catattcgcg gcaaacccga cccactcgcc gttgtaaaaa atcggcgtga
     9061 acacgcccat atcaggcgca tgcgggctgc cgccgagata agggtgattg gtgatgaagg
     9121 catcgccttg ctgaatctcc gccaactcgt aggtccctaa tatggcttcg gcgcaagctg
     9181 ggaatgcgcc catgtgcagc ggcagcacga cgtgttgcgc gaccacttcg ccctggcggt
     9241 tcaatatcgc gcaactggcg tcttgtgatt cgcggatgat ggtggaatag ccggtccgaa
     9301 aaagtgagtt ctgcatctcc tggacaatcc cggcaagccg cgcctggatg acttggagag
     9361 tgatcggatc aacgctcata tcagcaaacc gccggttcaa aacggtcgaa ccggtcaaaa
     9421 gggtgggaga aaatgccgtg cttcatgtct gggtgcgcag cgcgcgggtc tcggattcgt
     9481 tgaggatgaa gtcactgtct ctttcatcta cggccacccg gtaaacttcc cgtgcttttg
     9541 ctcgcgtcac ataaccattg cgcacatcgc tgagaactcg ttgaggatct ctttgcaacg
     9601 gatttccgag tccgccgccg cctggagttt ccaggctaaa aacatcgccc ggatgcagaa
     9661 cgtggtcgga gaagcgcgac gggatcgccc gctcgtccgg cttgccgggg tttagcgtgc
     9721 agtggccggt cttgccgttg ccgccgccgt cgataccttt aggggcaacc gtgtgtttgg
     9781 tggatcgcag tgaaaaacgc gcgtcgttgt ccaagacttc gtattctcgg acaaacccca
     9841 agccgccgcg aaatgttcct gggccgcccg aatccggaat cagcgcaaat cttcgcaagc
     9901 gcgtagagaa ctcggattcg atgatttcaa tcggcgcgat tttggcgttg gactgattga
     9961 cattggtgcc ggaaacgccg tctttgccgc tgcgaccgcc agagccaccg ccgaagattt
    10021 catactgcac gtagcttttg ccggttttat tactgcggcc gccgatgatg atggaccggc
    10081 tgctgcaacc gtcgcccacc tttttatgag gcgtgatctg gctcgacgct tccagcaatg
    10141 attcgaccaa cgcatggacg gtcggattat acgtgtttac cggagcggga aagcgcgggt
    10201 tgacgacact gccttcgcga accttgatgt cgataacctt tgccaatcca taattgatgg
    10261 gtagcgccgg atctaccagg caggtgagaa gatagcagca cgccgcgcgc acaatcgtcg
    10321 ggcgaatatt cgccggacct ttggtttgat cggcgcaacc ggtgaaatcg aacagcagct
    10381 ctcggccttt cttttccatg cgcacatgca agcgcaccgg cgtatccaga tcgatcccgt
    10441 cgctatcgac gaaccgctcg ccttcgccaa cgccgtcggg ccaggaggcg atttcagtgc
    10501 gcaccttggc ttcagtcaat gcaaacaatt gctcaaacga gctgagaatg gtttctttgc
    10561 cgtagcgctc catcatctct tggaagcgat tttcgccaag ccgcgcggcg ccgacttggc
    10621 cgcgcaggtc gcccaccacc agctccggag tgcggctgtt cgcgcccaaa acggcttcga
    10681 tgtctttgct ggtttgatag ttgtgaacga acttgatcgg cggaacgtga agaccttcct
    10741 ggtaaatttc tcgggcctga ctccagcagc ttcccggcac gggaccgccg atatcgcttt
    10801 tatgcgcgag atttgccgca aagccgaccc actcgtcgtg aaaaaagatc ggcgacaaca
    10861 ccgccatatc gggagcgtga gggctgccac ctaaatacgg gtgattggtg atgaaggcat
    10921 cacctttgtg aatttcttct gcgctgtagt ttttcaagat ccccgcggcg caggccggaa
    10981 atgctcccat gtgcagcggc agtaccacat gctgtgcgac gacttcgccc tggcgattca
    11041 ggattgcgca actggcgtct tgtgactcgc ggatgatcgt tgaatagccg gtgcgaaaaa
    11101 gtgaattctg catctcctgg acgatgccgg ccaaacgtgc ctgaatcacc tgcagggtta
    11161 cgggatcaac attcatagat tcgaactcgt tgctagtgga ctccgactaa acgattggaa
    11221 acactggaac agatggaacc ggcgattgag atcgtttcaa ccgttcattg cttcccgtca
    11281 tcaaagccct attgctgctt gctcctcatg atcagattcc gataatcatc cacggtgact
    11341 tcctgctccg ggtgaatgac cgtcgttgta tccaactgct cgacaattgc cggaccgctg
    11401 agcccgtgac ccggttcgag cgcatcacgg ctatagacct gggtcttcaa catcccgtgc
    11461 tccttgccaa aaaatacttt tcgcgttccc gtccttgctt cttcgaccgt gcgcccggaa
    11521 actttaccgg gtgaaagctt ggcctgcggg accattccaa gtgaaatcaa ccgaaggctc
    11581 accagttcga cgggctcggt ctctgccttg tgaccggagt tctgttcgtg gaccgtatcg
    11641 aagcgagcgc gcatcaacgc caagtcgtcc ttgttcagcg gcggcatggg gcaggggacg
    11701 gtcagctcgt aaccctgacc ggcgtagcgc agatccagat agggttccat tctgatttca
    11761 ctatcgcgaa aaccctcacc gtaaagatcg gcttttgctt gttcgatcag ctgcccgaac
    11821 atcgggttga tctcgtccag atcgagatcg tcgagtccaa ctaatttgga tcggacgtag
    11881 tcatgcttaa catcggacat gagcaacccc agagctgaat gaacgccggg cgtgagcgga
    11941 atcagcaccg aaggaatgcc taaatccaga gccatcttgc ccgcatgcat gggcccgccg
    12001 ccgccaaagg cgactagagt gaagtcacga atgtcataac cccgctggga cgatactgcc
    12061 ttaatcgcct cttccatttt gacattgatg atgtcgagca ccccgtgtgc tgcttccatc
    12121 aaatccattg tcaaaggttt ggcgattttt tcctccagca gacgctgggc tttcgcttta
    12181 ttgagcttca tgcggccgcc gagaaaatga tccggatcca atacaccaat caccatgttg
    12241 gcatctgtga tggtaatgtt ttcgccgccg cggtcatagc agaccggacc cggatcggcg
    12301 ccggcactat ccggtccgac ctgtaaaccg ccaacggcgt caagcctggc gatggtgccg
    12361 ccaccggcgc tgaccgtgtg aatgtctagc atcggtagcg cgattggccg cagattgatt
    12421 tttccttgcg tcgtgatgac tggattgccc ttatgaatca acgccacgtc gcagctggtg
    12481 ccgcccatgt cgaaggtgat gatgttgttt atgccaacgc gctcacaagt tcccatagaa
    12541 gcgatgacgc cgccggccgg gcctgaaaga accgtagcca ccggcttttt ggctgagccc
    12601 gcaaatgtcg agacgccgcc gttggattgc atgatataga gcttgggggt ggtgataccc
    12661 atctctcgca tgcggttttc aagcctgccc aagtagtttg ccatcaccgg ctggatatag
    12721 gc
//
LOCUS       DASUTG010000042         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_154529, whole genome shotgun sequence.
ACCESSION   DASUTG010000042 DASUTG010000000
VERSION     DASUTG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154529"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF089_00905"
     CDS             <1..422
                     /locus_tag="VF089_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6172541.1"
                     /translation="LNRNVPIVFMTGHGNIPMSVQAMKAGAVDFLPKPVKDTDLLRAI
                     EQALARAVRDRAERNELEDVRERVEKLTPREREVMVLVVRGLLNKQIAFELGTVEKTI
                     KVHRARVMEKMQVDSLADLVRLAEKVGVPARDRVPEQ"
     gene            1004..2719
                     /locus_tag="VF089_00910"
     CDS             1004..2719
                     /locus_tag="VF089_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172542.1"
                     /translation="MTESMNLCRRRPVSLSWVVFAVAIVVFQPVVAFAAQPAYEPSTT
                     LTASQILPPELLAGPNYRVEERVSNDGYLNTYRVVSKFGTAVAVSKPMLRKRISEINA
                     LVRMEQIQGTKEFTSSLREAGTDTLVGFKNLVTHPVDTVKGAASGLAVAFRRAGDQLT
                     GPKRSEAEDSRIKDLIGFSKTKREYAYQLGVDAYTDNEKVQDRLNEISWAGYSGGITW
                     AAAMAAVPGGAGTAITISGTHKLLNEVFRTTPPVDLRRMNGEKLNAMDVHPEVADAFI
                     NNSVYSPRYQTLLVHAMEEMKGVGNRATFVRLAAATANKDLALFRERQAEMYAGYHKA
                     VAPVETFLALGEFAAVRTSANGIVFNVPLDHLVWTDAMAKLLTAADARVTQLTRPASK
                     QLWVTGTVSARAKKEIEARGWQVHEQSEDRLLSWSEDYPKYEKPEDRMPAGLVKLNFK
                     SVAVGVGGSSGDGVLSYQGKDYPFTISGLNFVDVGVSNFEGAGKVYELKNLNDFAGNY
                     AAALAGFAIAGGQSELSMRNGKGVTVIVLANEGKESGTRLSLGPSGVTFKLKQKPAPL
                     DKLKA"
     gene            2734..3867
                     /locus_tag="VF089_00915"
     CDS             2734..3867
                     /locus_tag="VF089_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6172543.1"
                     /translation="MEPEGQISFGREDATTLRVRFSGTWRLRRGLPTAALVERELKSA
                     GHIRSIAFDADELAFWDSSVLVFLVDVSELCRERGVTMDRASLPTGLRRLLDLAEAVP
                     EKKGARQEAVQTAFLERIGNTAIGCGTSLGEMLAFLGDMTITFVRLFRMKVRFRAVDL
                     FLLIQQAGAQALPIVTLISFLVGVILAFVGAVQLKQFGAQIYVADLVGIAMIREMGAM
                     MTGIIMAGRTGAAVAAQLGTMKVTQEIDAFTTMGFSPLEFLVLPRVIALVLMMPLLCL
                     YSDFVGVLGGAAIGVGMLDLSWTTYFRQTANAISLPDVFGGVFKSSVYGVLIALAGCL
                     RGIQCGNSSSAVGDAATSAVVTGIVAIVVACGLFAVLFYLLGI"
BASE COUNT          892 a         1062 c         1148 g          926 t
ORIGIN      
        1 ctttgaaccg caatgttccg attgttttta tgaccggcca cggcaacatc ccgatgagcg
       61 tgcaagccat gaaggctggc gccgtggatt tcctgccgaa accggtcaag gacacggacc
      121 ttttgcgcgc cattgaacag gctctcgccc gcgccgttcg tgatcgcgcc gaacggaatg
      181 aacttgaaga tgtccgagag cgcgtggaaa aattgactcc gcgcgagcgc gaagtgatgg
      241 ttctcgttgt caggggattg ttgaacaaac aaatcgcctt cgaactcggc acggtggaaa
      301 aaaccatcaa agttcatcgc gcgagggtga tggaaaaaat gcaggtggat tcgctcgccg
      361 atctagtgcg cctcgcagag aaggttgggg taccggccag agacagagtt cctgaacagt
      421 gaagccttag cgcagccatc gtagtcctcg atctccaccc tgcttccatc gttcacaggt
      481 attgagctct tcttcgagat tcggatacaa ccctcatcct tcaccttcct actcttccac
      541 tcctcatcca cccgccgagg cattggacta aggtccaata tacggctccc tttaccttta
      601 atagagttca ctccgtacaa ctaattctta atcacggttt acaaccgaac cgggcgcatc
      661 caagcgggct ctggcaagcg aggggtagct ttcttgcgca agccgttcaa agacgaattg
      721 ctcatcgacg cggcgcagaa ccttcgacgc actggacaag agggtgaagt gaatgccgga
      781 ttctttcgtt gatcaaataa gtggcgcacc ccgtatagca gaagaaaaaa attccaagca
      841 tcaaatccga agctcgaaat tttagatgaa tgaataaaca caaagttact aagcaggctt
      901 ctaattcgaa gtggacgggt gtttgtcatg cgaatttaag ctttagagat tgtttccatt
      961 ttcgagtttc aaatttcggt tttagattag aaggagcttt tgaatgacag agtcaatgaa
     1021 cttgtgtcga cgtcgaccag tcagtctatc gtgggtggta ttcgccgtcg cgatcgtggt
     1081 ttttcagccg gtggtcgcgt tcgctgctca gcccgcctac gagccgtcca caacgctaac
     1141 cgcgtctcag atcctcccgc ccgagcttct agctggtccg aattaccggg tggaagagag
     1201 agtctccaac gatggttatc tcaatactta ccgagtggtg tcgaaattcg gcacggctgt
     1261 cgccgtatca aagcctatgc tgagaaagcg catcagcgag atcaacgcgc tcgtgcgcat
     1321 ggagcagatc caaggcacta aggaattcac gtcatcgctc agggaagcag ggacagacac
     1381 tttggtgggt ttcaagaatc ttgtcaccca cccggtggat acggttaaag gtgcggcttc
     1441 ggggttagcg gtcgcctttc gaagagcagg cgaccagctc acggggccca agcgcagcga
     1501 ggcggaggat agccgaatca aggatctgat cggtttttcc aaaaccaagc gggagtacgc
     1561 ctatcaactc ggggtcgatg cctacaccga taacgaaaaa gtccaagacc ggcttaacga
     1621 gatttcctgg gccggctatt ccggcggcat tacgtgggcg gccgcgatgg cggcagtgcc
     1681 ggggggcgcc ggcacggcga tcacgatctc gggaactcac aaactgttaa acgaggtttt
     1741 tcgcaccacg ccgccggtcg atctgcggcg catgaatggc gaaaaactca acgccatgga
     1801 cgtgcatcct gaggtcgccg atgcgttcat aaacaattcg gtatattcgc cgcgctatca
     1861 aacgctcctg gtccacgcta tggaagagat gaagggcgtg ggcaaccgcg ccacgttcgt
     1921 tcgactcgcc gccgctaccg ccaataaaga tctcgctttg ttccgtgagc ggcaggccga
     1981 aatgtatgcg ggctaccaca aggccgtggc gccagtggaa acttttctcg cgcttggcga
     2041 atttgccgcg gtacgaacca gcgccaatgg aattgttttt aacgtgccgc tggatcatct
     2101 cgtttggacc gacgcgatgg cgaaattgtt gaccgccgcc gatgcgcgtg tgactcaatt
     2161 gacccgcccg gccagtaagc aattgtgggt tacgggtacc gtgagcgccc gggccaagaa
     2221 ggaaattgaa gctcgtggct ggcaggtcca cgagcaaagc gaggaccggt tgctcagttg
     2281 gagcgaagat tatcctaaat acgaaaagcc ggaagataga atgccggcgg gtttagtcaa
     2341 gctgaacttt aagtctgtcg cagtgggcgt tggcggcagt tccggcgacg gcgttctcag
     2401 ctatcaagga aaggattacc ccttcacgat ttccgggctt aacttcgttg atgtcggcgt
     2461 atccaacttt gaaggtgccg gcaaggttta tgaacttaaa aacttgaacg actttgccgg
     2521 caactatgca gctgccctgg caggctttgc cattgcgggc ggtcaaagcg aactatccat
     2581 gcgcaacggc aagggtgtga cggtcatcgt gcttgctaac gaaggcaaag aatccggaac
     2641 acgcctgagt ctcggtccat ccggcgtcac tttcaagctc aaacagaagc ctgcgccctt
     2701 agataagctt aaggcataag ccgagacggc cgaatggaac cagaaggtca gatttccttt
     2761 gggcgcgaag atgctacgac gttgcgggtg cgtttttcgg gcacgtggcg gttgcgccgc
     2821 ggactcccca ccgcggccct ggtcgagcgc gaactcaaat cagcagggca tatccgcagc
     2881 atcgcttttg acgccgacga gctggctttc tgggacagca gcgttttagt cttcctcgtt
     2941 gacgtttcgg aactatgccg tgagcgcggc gttaccatgg accgcgcgag cttgcctact
     3001 ggtctgcgcc gattactgga cttggccgaa gcggtgccgg aaaaaaaagg cgcgcgccaa
     3061 gaggcggttc agacggcttt tcttgaacgc atcggcaaca ctgcgattgg ctgcggcacg
     3121 tcgctcggag agatgctggc gtttctcgga gacatgacga ttacctttgt ccggctcttc
     3181 cgtatgaagg tgcgattccg cgccgtggat ttgtttctct tgatccaaca agccggcgct
     3241 caagcgttgc cgatcgtcac attgatcagt tttctggtgg gcgtgattct cgcgttcgtc
     3301 ggtgccgttc aactcaaaca attcggagca caaatctacg ttgccgatct ggtcggcatc
     3361 gccatgatcc gtgagatggg cgcgatgatg acgggtatca tcatggcggg ccgcacgggc
     3421 gcggccgtcg cggcacagct cggcacgatg aaagtgactc aagagatcga cgccttcacc
     3481 accatgggtt tttcgccgct ggaatttttg gtgctgccgc gcgtgatcgc gctggttctg
     3541 atgatgcctt tgctttgtct ttattccgac ttcgtcggcg tgcttggcgg cgccgctatc
     3601 ggtgtcggga tgctggatct ttcctggacc acatattttc gtcagactgc caatgccatc
     3661 agcttgccgg atgtgtttgg cggcgtattc aagagctcgg tttacggcgt gctcattgcc
     3721 cttgcgggtt gtttgcgggg gattcagtgc ggcaacagtt cctccgcggt gggcgatgcg
     3781 gcgacttccg ccgtggtcac ggggatcgtc gccatcgttg tagcctgcgg tctctttgcc
     3841 gtcctgttct acctattggg gatctgactt gaccgaagcg aaaggaacaa ggtgaaaact
     3901 cgaaactcgg aatccgaaac tcaagacaat tcaaatgatc aaaaagcaaa attccaaaca
     3961 aactactcat ggatttcgga ttttggattt gagtaatttc ccgtttgttt cggacttcga
     4021 gcttcgga
//
LOCUS       DASUTG010000043         7949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1933604, whole genome shotgun sequence.
ACCESSION   DASUTG010000043 DASUTG010000000
VERSION     DASUTG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933604"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..872)
                     /locus_tag="VF089_00920"
     CDS             complement(183..872)
                     /locus_tag="VF089_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6172544.1"
                     /translation="MNKTRIEQNQIDRIVQIPANGIELEGALMIPSNANGVVLFAHGS
                     GSSRHSPRNNFVAQVLHRAGMGTLLMDLLTTEEDSIYENRFDIDLLTWRLERATQWIM
                     EQPRTTSLGIGFFGASTGAAAALQAAATFGSSIGAVVSRGGRPDLAMSALDRVQSPTL
                     LIVGGLDDVVIDLNRKAYAKLKAEKHLAIVPGATHLFEEPGTLQEAAGLAAEWFKRHL
                     GSSRVQETSAD"
     gene            complement(953..1150)
                     /locus_tag="VF089_00925"
     CDS             complement(953..1150)
                     /locus_tag="VF089_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6172545.1"
                     /translation="MLTRDPVRISVSRNMVSLMHSQHVPRVPIVDGSDATLGMVTMDD
                     LIALLGDEMSELGQAVSQGFR"
     gene            complement(1230..1658)
                     /locus_tag="VF089_00930"
     CDS             complement(1230..1658)
                     /locus_tag="VF089_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6172546.1"
                     /translation="MSLKSYCRKPVIRISPETSITEACQLMEQNNIGCLIAEQEGKLC
                     GIVTDRDIALRVTGSKRDPDKTKVQDIMTPDPIRVSVDKDLHHLTALMHAYHVRRVPI
                     VNGFDTTLGLVTLDDLIAQLGSDISEIGKAISEEFPEISA"
     gene            complement(1699..2319)
                     /locus_tag="VF089_00935"
     CDS             complement(1699..2319)
                     /locus_tag="VF089_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6172547.1"
                     /translation="MIVGNRMTKEPITVGPDDLLIRASHKMQAGGFRRLPVMKEGKLV
                     GIVTERDLREHRGHLEHTKVNGVMTEDPITVSPATTLEEATKILLERQVGGLPVVADG
                     RLIGIITASDILNAFLDVMGASHGGSTRMDLVLEGEEHGLVEATRIVAREGGEVLGIG
                     TYREKPGEDPICYLRLISGTPEKISKALRASGFKVLGTHRIATKEV"
     gene            complement(2428..2601)
                     /locus_tag="VF089_00940"
     CDS             complement(2428..2601)
                     /locus_tag="VF089_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6172548.1"
                     /translation="MPSYEFRCEKCDKAFEKIWSLSEYDKRIKENNKCPSCGSTKIVR
                     TISLVQVKTSKKS"
     gene            complement(2620..3342)
                     /locus_tag="VF089_00945"
     CDS             complement(2620..3342)
                     /locus_tag="VF089_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6172549.1"
                     /translation="MNKKATIKQVRAVLEKDPRINLHRYPIAIAAQNGDLILTGEVES
                     IAAKKLALVAASQTHGVGRIVDRLKVTPAEKMGDAEIRDHVCKVLFEEPALERCLIQG
                     LVGNDVATARKATGGPAGSIVVAVSDGTVTLNGEVPSLSHKRLAGVLAWWVPGTRDVV
                     NGLEEVPAEEDNDDELTDAVRLALEKDPFVDASKIRVTTKEWVVTMDGLVPKETMKQM
                     AERDAWYVLGVSEVINRIQAER"
     gene            3684..3947
                     /locus_tag="VF089_00950"
     CDS             3684..3947
                     /locus_tag="VF089_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172550.1"
                     /translation="MEDADIEQRFHLMQKQIEATVSLTRECKLDLMAAIDSLRIELEV
                     LKSYMERYHPGFAESYPKFREEAIQAIDPEWIGSKPQKRLEGR"
     gene            complement(4179..4424)
                     /locus_tag="VF089_00955"
     CDS             complement(4179..4424)
                     /locus_tag="VF089_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6172551.1"
                     /translation="MAMATYEFHCQKCDLNFEPMFSFGQYQKKPDRSGAPIVIAPGWF
                     GGISLFEDQNFRKRVSPVGGPANVVNPRIPRRPDERA"
     gene            complement(4615..>7949)
                     /locus_tag="VF089_00960"
     CDS             complement(4615..>7949)
                     /locus_tag="VF089_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX6172552.1"
                     /translation="IAAPPHQAPPDGPMVLGVDAGSTTTKAILIDPGTSGVVASYYTR
                     TKGDPVAATRECLRALVDGIGNRDIGLIATTGSARELAGAYLGTEHVYNEISAQAAGA
                     AHFAGDVDTIFEIGGQDSKYIYLRNGVPIDYAMNNACSAGTGSFLEESAHGDLGIDVS
                     EIAELALAAPAPVQFKATCAAFINSDIRLALQEGQSREDIVAGLVYAIAGNYLNRVKG
                     GRYVGKNVFLQGGVALNHAIAHAFAHSVDRQVVIPPSPELLGALGVALLGLQRARGSL
                     ASATELLSLAEPEMKRTSRFTCGACNMYCSIDHFEVAGRRFPFGGRCSLYENVWKRKR
                     RTAAAPDLVEQRRKLLFGRPNYNSPANGKRIGVPKALTTHSLYPLYSTFFAGLGMDVV
                     LSEIDSRGELQSNAGFCFPAQIAHGAVLDLVKRGVELIFLPHVMRMPRGEASKESYLC
                     PITQASPYFLAKAYPQIHLLSPLLDFSNGYTDCSALVEMAVNELAQTRELAERQWTAA
                     VKVQTDAERDLEQLGQAALGQAIAGDKPAIVLAGHSYNAFTPEGSQSVGKKLSSMGIA
                     AIPADCLTPAAAGPTSWHFANQVMNAVEVVKQNPNLFLLCVSNFSCTIDAFTQSMLAS
                     EMGSKPYLILEIDAHTADAGVQTRLEAFLDIVKNHREGQVRRRDSFNPARLIAGGQVS
                     RGNGEQIAITDPRIKLYFPNFSQFHAESLAMSVGWLGLHAGPVIPLDRRHLSRGLQFT
                     SGRECLPLPICLGQLLEIHERRAPGEIAGFYMLQGGAPCVIDAYMSYLERFIVEQQLD
                     DLFLLTPGGENELCGYDAITLAKYTLPALVAADIMVELEQVMHVVAEPDASAQLKQEW
                     ESFIASAISLEQFHAQLPAFVDRLASLPRKRNPLDCPRIVVTGDFFTRFSPFFMEGVR
                     EIYNDRGIILKPVDLNGLLLYGAYDRISEAAGTWGIKPGGAALAKACTKIFQPDGKEY
                     LRRWLAYKSQKRTEAYYRKIFHESGYLLAQHGNAAATFADASEHISPKIYGEVIPTVG
                     DGVRAQVDGYDGIIIIGPFNCLPFRIAEAVLKPLSIRSGMPILTYESDGYAVSPSFIR
                     QVEVHAQQVLEHRARNPIHNGA"
BASE COUNT         1678 a         2223 c         2200 g         1848 t
ORIGIN      
        1 gatgcccgcg aaaagcccgc ggaatgatag ggatgtgagg gggctcgact tctaaccgtc
       61 tgcggaaacg cggtggattg cttcggcccg ctcagcatga gcggtattcc tgaacgattg
      121 caccttctcc gctggtcctg agcctgtcga aagactcttg tttttcacca atccggatct
      181 ttttagtctg cgctggtttc ttggacgcga ctgctaccga gatgccgctt gaaccattcc
      241 gctgctaaac cagccgcttc ctgcagcgtg cccggctctt caaataaatg cgttgcaccg
      301 ggaacgatgg ccagatgttt ttcagctttg agtttggcat aagctttccg attgagatcg
      361 atgacaacgt catcgaggcc gccaacgatt agcagtgtcg gcgattggac gcggtccagc
      421 gccgacatgg ccaaatccgg acggccaccg cgtgacacta ctgcgccaat cgatgaaccg
      481 aacgtcgctg cggcctgcag cgctgccgcc gcgccagtgc tcgccccgaa aaatccgatg
      541 ccgagggagg tggtccgcgg ctgctccatg atccattggg tggcacgctc gaggcgccag
      601 gtcaggaggt cgatgtcgaa tcgattctca tagatcgaat cttcctctgt ggttagcaga
      661 tccatgagca acgtgcccat ccccgctctg tggaggactt gagccacaaa gttgtttctc
      721 ggactgtgac ggctgctgcc gctgccgtga gcgaacagca cgacgccgtt ggcgtttgaa
      781 gggatcatca gcgcgccttc tagctcgatc ccgttcgccg gaatctggac gattcgatcg
      841 atctgattct gttcgatacg tgttttattc atcgtggcac caccaagtcg cttacgggtt
      901 gccgtcgtcg ggctggcttc gctccatatt gataatacgt ttccttagcc tgctagcgaa
      961 acccttggga gaccgcttga ccaagctcag acatttcatc gccgaggagg gcgatcagat
     1021 catccatggt gaccattcca agagtggcgt ccgagccatc aacgatgggt actcgtggca
     1081 catgctggct gtgcattagc gacaccatat ttcgactcac ggagatccgc accggatctc
     1141 gcgtcagcaa gcctctgcga tactcctctc cggagaatcg gtcactatgg gcttttgaca
     1201 atgctcgtca agacaagata gccctgccat taggcgctta tttctggaaa ctcttcggag
     1261 atcgctttgc cgatctccga aatgtcgcta cccaattgcg cgatcagatc gtccagtgta
     1321 acaaggccca gagttgtatc gaagccgttg acaatcggta cgcgccggac atggtaggca
     1381 tgcatcagcg cggtcaagtg atgcagatcc ttatcgacgg agacgcgaat cgggtccggc
     1441 gtcatgatgt cttggacctt tgtcttatcc ggatcgcgct ttgagccagt gacccggagc
     1501 gcgatgtctc ggtcagtgac gatgccgcaa agcttcccct cctgctcggc aattaagcaa
     1561 ccgatattgt tttgctccat cagttggcag gcttcggtaa tgctcgtctc cggcgaaatc
     1621 ctgattaccg gttttcgaca ataacttttc aacgacatag attcctcccg taaaagagcc
     1681 ctgtctccat ttcatttgtc atacttcttt cgttgctatc cgatgtgtcc ccagcacctt
     1741 gaagccgctc gcgcggagcg ccttggagat tttctcggga gtccctgaga tcagacgcaa
     1801 gtagcaaatc ggatcttcgc caggcttttc tctgtaagtg ccaatcccta aaacctcgcc
     1861 gccttcgcgt gcaacaatgc gcgtggcttc gaccaagccg tgctcttcgc cttctaaaac
     1921 caagtccatc ctggtgctac cgccgtgcga ggctcccatc acgtcaagga atgcgttgag
     1981 aatatcactg gcggtaataa tgccgatcaa gcgcccgtct gccactaccg gcaggccacc
     2041 cacctgccgt tccagcagaa ttttcgtcgc ttcttccaat gtcgtcgccg ggcttacggt
     2101 tattggatcc tccgtcatca caccgttaac tttcgtgtgc tcgagatgac cccggtgctc
     2161 gcgcaggtct cgttcagtga cgatgccgac aagcttgcct tccttcatta ctggcagtcg
     2221 gcgaaagccg cccgcctgca ttttatgcga ggcgcggatc agcaaatcat ccggcccaac
     2281 tgtaatcggt tctttagtca tgcggtttcc aacgatcata ttgacctcac ggttctcttt
     2341 gttgcttgac tagttcccga agcaaatccc gttgggcaac gagtccggcc aagcggttac
     2401 tgtcgatcac gtcatggctt attgtcatca actcttctta gacgtcttca cctgcaccag
     2461 cgaaatcgtc ctgacgattt ttgtgctgcc gcacgaggga catttattgt tttctttgat
     2521 ccgcttgtcg tactcggaga gggaccagat tttttcgaag gccttgtcgc acttctcgca
     2581 ccgaaattca taactcggca tgtggcgtcc tcctgctcct cagcgttcgg cctggatccg
     2641 gttgatgact tcactgaccc caagcacgta ccaggcatcg cgttcggcca tctgcttcat
     2701 ggtctctttc ggaaccaacc catccatagt gacgacccac tctttggttg tgacccggat
     2761 cttgctcgca tcgacgaatg gatctttttc caaggcgagg cggaccgcgt cggtcagctc
     2821 atcgtcgtta tcttcttccg cgggcacttc ctccaagccg ttaacaacgt cgcgagtccc
     2881 cggtacccac caggcaagga cgccggctaa gcgcttgtgc gataggctag gcacttcccc
     2941 gttgagcgta actgtgccgt cgctgactgc gacaacaatc gacccggcag gtccgcctgt
     3001 agcttttcga gcggttgcga catcatttcc gaccaagccc tggataaggc agcgctccag
     3061 cgccggttct tcgaacagga ccttgcacac gtgatcacga atttccgcgt ctcccatttt
     3121 ttcagcggga gtgaccttca gccggtcgac aattcgtcca acaccatgag tctgcgacgc
     3181 cgctacaaga gccaactttt tggcggcgat actctcgacc tctcccgtca ggatcagatc
     3241 gccgttttga gcggcaatgg caatcggata gcgatgaagg ttgattcggg gatccttttc
     3301 gaggacggca cggacctgct ttatggttgc ttttttattc atcgcctttg cactttcggg
     3361 ttccacctaa cgacgagatt gcctgcctcg ccgcgtccgt acaatagttt tggtttgtat
     3421 ggcattccga ttcctgcgca aagcatgtgc cattacactt gattagcgtt gatagggatc
     3481 tattggatca gaacagatcc aggtctctga agtcgactcg cttttttcaa tcttgatatt
     3541 ttctaacccg tcaatttctt agcggcggga attaagttag ggaggctatc accggcgaaa
     3601 ctaattcaaa tagtaagttt atctggcacg ctggatgcgg gctctgatgt ggtctgtaag
     3661 agaatttcgc ctggaggtgt cgtatggaag atgcggacat tgagcaaaga tttcatttga
     3721 tgcaaaagca gatcgaagcg accgtttcgt taacacggga atgcaaactc gatctgatgg
     3781 cggccattga ttcacttagg atcgagctgg aagtcttgaa gagctacatg gaacgctatc
     3841 atcctggttt tgcggagtct tatccaaagt tcagggaaga ggcgattcag gcaatcgatc
     3901 cagaatggat tggctcgaag ccccaaaaaa ggcttgaagg ccgctaatcc ggtcggcctg
     3961 caggcggatt tgcaatgtgc agaaatcatc gttaaggccc ttgatgagac ggcaaaaatg
     4021 tctcgcagct cgggaccgct acatgcattt cgcaccaatg agagaaacag gcggcgcgat
     4081 tcgcaaaggt gattaagcca cacgatatcg cgacgaaaac tcggaccgat taatctcgaa
     4141 tcgaattcgt cgcccaattg taagtttttc tttgcccatc atgcacgctc atcgggccgg
     4201 cgcgggattc ttggattgac cacattggcc ggccctccaa ctgggctaac tctttttcga
     4261 aagttttgat cctcaaataa tgagattccg ccgaaccacc ctggtgctat tacaattggg
     4321 gcacctgatc ggtctggttt tttctgatac tggccgaatg aaaacatcgg ctcgaagttc
     4381 aggtcgcact tctgacaatg aaactcatag gtggccatgg ccatgctacc tccgtgatgt
     4441 ccatctaaaa gttcgtcgtc tccggctcat ttagattcag cagacggtgt gccaactgag
     4501 gggcaggcat tgcagaagac caagcttaga tcttcgcttt tgcgaacgga aaaggaggag
     4561 gtaaggcaag ccgattacaa gataatgaca cggcaaggcg gacctgcggg ccgttcacgc
     4621 tccattatga atcgggtttc gtgctctgtg ctcgagaacc tgttgcgcgt ggacctctac
     4681 ttgtctaata aaggacggcg acaccgcata accgtcgctt tcgtaggtga gtatcggcat
     4741 gccactacga atgctgagcg gcttgagaac ggcctcggcg atgcggaacg gtaagcagtt
     4801 gaacggaccg ataatgatga tgccgtcgta gccgtccacc tgtgcgcgca cgccgtcgcc
     4861 gacggttgga atgacctcgc cgtagatctt gggtgagata tgttccgatg cgtccgcaaa
     4921 tgtagccgct gcgtttccgt gctgtgccag cagatagccg ctttcgtgaa agatcttgcg
     4981 gtagtaggct tctgttcgct tctggctctt ataggcgagc caacggcgca aatactcttt
     5041 cccatccggc tgaaaaattt ttgtacatgc tttcgccagt gccgcgccgc ccggtttaat
     5101 tccccaagtg ccggctgctt cagagatcct gtcatacgca ccatagagca gcagtccgtt
     5161 cagatcgaca ggtttgagaa tgatgccccg atcattgtag atctcgcgca cgccttccat
     5221 gaagaatggg ctgaagcggg tgaagaaatc gccggtgacc actatccggg ggcaatcgag
     5281 ggggtttcgc ttgcgcggca gggatgccag gcgatcgacg aaagcgggaa gttgcgcatg
     5341 gaattgctcc agtgagattg cgctcgcgat gaagctttcc cattcctgtt tcagctgcgc
     5401 cgaggcatcc ggctcggcga ccacatgcat cacctgttcg agctcgacca tgatgtccgc
     5461 cgccaccaga gccggcaggg tgtacttcgc tagcgtgatg gcatcgtagc cgcaaagctc
     5521 gttttcaccg ccgggagtaa gcaggaataa atcgtccaac tgctgttcaa cgatgaaccg
     5581 ctccagatag ctcatgtagg catcgatcac acagggcgct ccgccctgca gcatatagaa
     5641 gccggcgatt tcaccggggg cgcgccgctc gtgaatctcg agcagctggc ccaggcagat
     5701 cggtaatggt aggcattccc gaccggaggt gaattgaagg cctcgactta ggtgacggcg
     5761 gtcgagcggt atcaccggac cggcgtgcaa gcccaaccag ccgacggaca tcgccagaga
     5821 ttccgcatga aattgtgaga agttggggaa atagagcttg atccgaggat cagtgatcgc
     5881 tatctgctcg ccgttgccgc ggctgacttg accgccagcg ataaggcgag ccgggttgaa
     5941 tgagtcgcgg cgtctgacct ggccttcacg gtgattcttc acgatatcga gaaacgcttc
     6001 caagcgggtt tgtacgccgg cgtcagcggt atgggcatcg atttcgagaa tcagataggg
     6061 ctttgagccc atctcggacg cgagcatcga ttgggtgaaa gcgtctatgg tgcaactgaa
     6121 gttgctaaca cagagaagaa aaagattcgg gttctgcttg acgacttcca cggcattcat
     6181 cacctggttg gcgaagtgcc acgaggtggg acctgcggcc gccggcgtaa ggcagtcggc
     6241 gggaatcgcc gcgattccca tgctcgataa tttcttgcct accgattgcg acccttcggg
     6301 ggtgaaagca ttgtagctgt ggccggcgag gacgatcgcc ggcttatccc cggctatcgc
     6361 ttgtcccagt gcagcttgtc ctagctgctc cagatcgcgc tcggcatcgg tttgtacctt
     6421 gacggcggcg gtccactgcc gttccgccaa ctcgcgcgtt tgtgcaagct cgttgaccgc
     6481 catttcgacc aaggcggagc agtcggtgta accgttgcta aaatcgagca gcggtgagag
     6541 cagatggatc tgcggataag ccttggccag gaaataggga ctggcctggg tgatcggaca
     6601 tagataagat tctttcgatg cttcgccccg tggcatgcgc atgacatgag gcaaaaaaat
     6661 cagctccaca ccgcgtttaa ccaggtccaa cacggcgccg tgggcgatct gcgcgggaaa
     6721 acagaagccg gcattcgatt gcagctcgcc gcgcgagtcg atttccgata acaccacgtc
     6781 catgcccaag ccggcgaaga aggtcgagta gagaggatag agcgagtgag ttgttagagc
     6841 ctttgggacg ccgattcgtt ttccgttggc tggactattg taattgggac ggccaaaaag
     6901 cagcttgcgc ctttgctcta ccaggtcagg cgccgctgcg gtgcgccttt ttcgtttcca
     6961 gacgttttca taaagactgc agcggccgcc aaaaggaaag cgtctacccg cgacttcgaa
     7021 atggtcgatg ctgcagtaca tgttgcaggc gccgcaggta aatcgacttg tccgtttcat
     7081 ctccggctcg gccagactca aaagctccgt cgcgctcgcc aacgagccgc gcgctcgctg
     7141 gagccccagc aaggctacgc ccaacgcacc cagcaactcc gggctcggcg gaatcaccac
     7201 ctggcggtca acgctatgag cgaaggcgtg cgcgatcgcg tgattaagag cgacgccgcc
     7261 ttgcaggaag acgtttttgc cgacatagcg gccccccttg actcgattga gataattgcc
     7321 ggcgatagcg taaaccagcc cggcgacaat gtcctcgcgc gattggcctt cttgcagggc
     7381 gaggcggata tcggagttga taaacgccgc gcaggtagcc ttaaactgca ccggcgccgg
     7441 agcagccagc gcaagctcgg cgatttcgga aacatcgatg ccgagatcgc cgtgggcgct
     7501 ttcttcgaga aatgaaccgg tacccgctga acaggcatta ttcatcgcat aatcgatggg
     7561 aacgccgttg cgcaaataga tgtatttgga atcctgtccg ccgatttcaa agatcgtatc
     7621 aacgtcgcca gcgaaatggg ccgcgccggc cgcctgggcg gaaatttcgt tgtagacatg
     7681 ttccgtgccg agataggcgc cggcaagttc gcgcgccgaa ccggtcgtgg cgatcaggcc
     7741 gatgtcacgg ttgccaatcc catcgaccag cgcgcgcaag cattcgcgcg tcgcggcgac
     7801 cggatctccc ttggtgcgcg tgtaatacga tgccaccacg ccggaagtgc cggggtcaat
     7861 taaaatggcc ttggtcgtgg ttgatccggc atctactccg agcaccatcg ggccatcggg
     7921 cggcgcttgg tgaggcggcg cggcaatga
//
LOCUS       DASUTG010000044         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_302986, whole genome shotgun sequence.
ACCESSION   DASUTG010000044 DASUTG010000000
VERSION     DASUTG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302986"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF089_00965"
     CDS             complement(<1..661)
                     /locus_tag="VF089_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6172553.1"
                     /translation="MRSSPTLIIFVSLLTAPIACSKTEAPPPPPPPEVQVAEVTQNDV
                     PIYIELVGATLGSEDVEIRARVEGYLVSMNFTEGSFIKKGQLLYKIDPQPFEAALAEA
                     TANLSTARARVDKTNNDVARLTPLAKQQAVSQQELDNAVSAQEAAQAQVAAYDAKVAK
                     TKLDLAYTTITSPIDGLIGTTQKKVGSLVAGGETLLNTVSQIHPIFFRCAIAEAEYLR
                     LA"
     gene            complement(923..>2678)
                     /locus_tag="VF089_00970"
     CDS             complement(923..>2678)
                     /locus_tag="VF089_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX6172554.1"
                     /translation="AVVIFSVFLGVAMIGLERKQTALDVFSIMKDALSKATKFVVSLT
                     PYGVFAIAATTAGTLNLEQIARIQVYLITYVMVSLLVALWVLPGLVSALTPFRYKDVL
                     GPTRDALITAFMAADLFIVLPILINACKELLERHRLVDESTKTLPDVIVPTSFNFPHT
                     GKLLSISFILFAGWFADAAVPVSEYPRLALTGLLTFFGSLSAAVPFLLDLFRIPADTF
                     QLFVATGVVNSRFGALLAAVHTVTVALLGSAAIVGSLRFSATRIARYLVATILLSVAT
                     IGGLRALFNTVLKPTFAGEELVYSMRPLMTGGDARLVKATELGETTEKPAGSRIDTVK
                     KRSLLRVGVFADRLPFVFLNGQRRLVGFDVEMAQLLGRDLGVEVEFVEMENLTEMPRL
                     LAIGFLDIAMTGMPVTPERAGEMLFSEPYLDETFAFVVKDYLREKFSGWASIRDLGIF
                     PVAIPNLPYYIDRVKARAPELKLEVVDSIKRIEAGLQEGTLEAVALPAERGSVLTLLY
                     PKYAVVVPEPGVIKIPLAYPVAGRDQDWVSFLNTWIELKRRDGTIDALYGHWILGKQA
                     GKRQPRWSIMRNVLHWVN"
BASE COUNT          602 a          758 c          768 g          550 t
ORIGIN      
        1 gggcgagtct taaatactcc gcttcggcga tcgcgcagcg gaagaagatc ggatggatct
       61 gcgaaaccgt attaagcagt gtctctccgc cggccaccaa gcttccgact tttttctgtg
      121 tcgtgccgat cagcccatcg atgggcgaag tgattgtggt gtaggccagg tcgagcttag
      181 tcttagcgac ttttgcgtcg tatgcggcga cctgagcctg ggcggcctct tgcgccgaga
      241 ccgcattgtc gagctcctgt tgactgacag cctgctgttt tgcaaggggc gttaggcgcg
      301 ctacgtcatt gttggttttg tcgactcgcg cgcgcgcggt cgaaagattg gccgtggcct
      361 ccgcgagcgc agcttcaaag ggctgaggat cgatcttgta taagagctgg ccttttttga
      421 tgaaggagcc ctcggtgaag ttcatggaca ccagatagcc ttccacgcgc gcgcgaatct
      481 cgacatcttc cgatcccaac gtcgctccaa ctagctcgat gtaaatgggc acatcgttct
      541 gtgtgacttc ggcgacttgc acctcgggcg gcggaggcgg cggcggagcc tctgttttcg
      601 agcaggcgat gggagccgtt aataaactga caaaaataat gagagtgggt gaactgcgca
      661 tcaatcgccc cagcctcaag cgaacgcttt catggttcac gtttatcccc cttgggttgg
      721 attcaccgat tgcacgatta tttgcgaact cctgcggagt gctttaaccc tcggtaagtt
      781 atcaaataac gatgatattc ctcgtcccca actttgtatc tcgtaattat cgatatgtcg
      841 cgcttcggta tattggacct tagtccaata gcggcacaga ggaaaaactt gccctctgag
      901 aatgcgctaa tctgcgacga gatcagttta cccagtgaag cacattgcgc ataatcgacc
      961 agcgcggctg gcgtttgccg gcctgttttc ccagaatcca gtggccgtag agggcatcga
     1021 tggtgccgtc tctgcgtttg agctcgatcc atgtgttgag aaaggacacc cagtcctgat
     1081 cgcgaccggc gacgggatag gctagcggaa tttttatgac gcccggttcc gggaccacca
     1141 ctgcgtattt cggatagagc aaagtgagca ccgagccgcg ttcggcaggc agagccacgg
     1201 cctccaaggt tccctcttgc aacccggctt cgatccgctt gatcgagtcc accacctcaa
     1261 gcttcagttc cggcgcacgc gctttgaccc gatcgatgta gtacggcaaa ttcggaatcg
     1321 ccacggggaa aatgccgaga tctcgaatgc ttgcccagcc ggaaaacttc tcgcgcaggt
     1381 aatccttcac cacgaaggcg aacgtctcat caagataggg ttcggagaag agcatctctc
     1441 cggcacgctc aggggttacg ggcatgccgg tcatggcaat gtcaagaaac ccgatcgcga
     1501 gcagacgcgg catttcggtc aagttttcca tctcgacgaa ttcgacctcg acgcccaggt
     1561 cgcggccgag gagttgggcc atctccacgt cgaagccgac cagtcgccgc tgcccgttca
     1621 agaacacgaa aggaagccga tcggcgaaaa caccgaccct caacaggctg cgctttttga
     1681 ctgtatcgat ccgcgatcct gctggcttct cggtagtttc cccgagctcc gtcgctttga
     1741 ccaggcgcgc gtcgccgccg gtcataagcg ggcgcatact gtagaccagc tcttcaccag
     1801 cgaacgtcgg cttcaacacg gtattgaaca aggctcgcaa tccgccaatg gtcgctactg
     1861 atagcagaat agtcgcaacg agatagcgag cgatgcgcgt cgcgctgaag cgtagagagc
     1921 cgacaatggc cgcgctgccg agcagtgcca ccgtcaccgt atgaaccgcc gcgagcagtg
     1981 ccccgaaacg cgagttcacc actccggttg caacgaacag ctgaaacgta tcggccggga
     2041 tgcggaacag atccaataaa aacggcaccg ccgcgctcag gctgccgaag aacgtcagca
     2101 gacccgtaag cgccagcctc gggtattccg ataccggcac cgccgcgtcg gcaaaccagc
     2161 cggcgaacag aataaagctg atcgataaaa gcttgccggt atggggaaaa ttgaaggaag
     2221 tgggcacgat cacatcgggc agggttttcg tcgactcgtc gacaagccgg tgccgctcga
     2281 gcagttcttt gcatgcattg atgagtatcg gcaggacgat gaatagatcg gctgccatga
     2341 acgcggtaat cagcgcatcg cgcgtcggcc cgagcacatc tttgtagcga aatggagtca
     2401 gagcagaaac caggccgggc agtacccaga gcgcgacgag caaagaaacc atcacgtagg
     2461 tgatcagata cacttgtatc cgggcaatct gttcgagatt caaagtcccg gcggtggtgg
     2521 cggcgatggc gaagacgccg tagggcgtca aactcacgac gaactttgtg gctttcgata
     2581 aggcgtcttt catgatcgag aaaacgtcca gcgcggtctg cttgcgctcc aggccgatca
     2641 tcgcgacacc gaggaagacc gagaaaataa ccacggcc
//
LOCUS       DASUTG010000045         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_896066, whole genome shotgun sequence.
ACCESSION   DASUTG010000045 DASUTG010000000
VERSION     DASUTG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896066"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF089_00975"
     CDS             complement(<1..378)
                     /locus_tag="VF089_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172555.1"
                     /translation="MALQERTGESSFDRYIEEVRAVWGDGKDPQLPYKVAALMEKLFA
                     STSANDPWMAQIIREGKPSRELYRDAEHGFIQMGHVHQSGHGNTPHDHGPCWVVYGAF
                     SGLTEITKYKRTDDGTQPGKATLK"
     gene            589..1068
                     /locus_tag="VF089_00980"
     CDS             589..1068
                     /locus_tag="VF089_00980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6172556.1"
                     /translation="MSIEPQELRRVMGHFATGVTVITTQDKDGNPNGLTANAFMSLSL
                     NPPLVLISVDKSAQCYACFNLQNGFTVNFLSEDQEEVSRRFATKGIDKFAGLQWHPGQ
                     NGAAILDAALGYVECKITQCYDGGDHTIVVGEIVNANATGERPLLFFKGKYQRLPDS"
     gene            1566..>2221
                     /locus_tag="VF089_00985"
     CDS             1566..>2221
                     /locus_tag="VF089_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172557.1"
                     /translation="MTQVVGRPTPRVEGELKVTGKALYSADVELPDILWGKVLRSPIS
                     SGRIKNIMTDKARQLPGVKAVITGEDVAGLRIGRCIYDHPVLADGLVRFIGEKVAAVA
                     AETRLAAEQALELIEVEYDEMEPLLDPVAAIKDDAPILHPDLLSYKGLPVPLEKVSNV
                     FAYLKWGKGDIEAGFRQAEVIVENTFTTQVTHQSYLEPHACVVRVDPSGGASIWSCSK
                     T"
BASE COUNT          509 a          587 c          602 g          523 t
ORIGIN      
        1 tttaagagtt gcttttcccg gctgtgtgcc gtcatcggtg cgcttgtatt tagtgatttc
       61 ggtgaggccg ctgaaagccc cgtaaacaac ccagcacggc ccatgatcgt gcggcgtatt
      121 accgtgcccg gattggtgca catggcccat ttggatgaac ccgtgctcgg cgtctcgata
      181 gagctcgcgc gagggcttgc cctctcgaat gatctgcgcc atccaggggt cgttggcact
      241 ggtgctggcg aacagcttct ccatcaatgc ggcgaccttg tacggcaact ggggatcttt
      301 gccatcgccc caaaccgcgc ggacctcttc gatatatctg tcaaacgagc tctctcccgt
      361 tcgttcttgt aatgccatcg gacacctcct tgaatggtta tctttaacat gttccgtaag
      421 gaatcgtcca gcacatgaat agaatgggaa gccttcggcg agtgctgaca atcggatcag
      481 cagagttgct catccgtccc cgacgcgcac gatcagttta ttttaccctg attgacagca
      541 tcaggttgac tctggtagca gtaaaaaacc ccgagaataa aagcaatcat gtcgatcgaa
      601 ccacaagagc tgcggcgggt tatgggtcat ttcgccactg gcgttaccgt gattacgacc
      661 caggacaaag acggaaatcc aaacggactg acagccaatg ctttcatgtc cctgtcactg
      721 aatccaccgc ttgtcctgat cagcgttgac aagagcgcgc agtgttatgc gtgctttaac
      781 ttgcagaacg gcttcacagt aaattttcta agcgaagatc aggaagaggt ttcgcgtcgt
      841 tttgccacca aagggatcga taagtttgcc ggcctgcagt ggcatcccgg ccaaaacggg
      901 gcagcgattc tcgatgcggc gctcggctat gtcgagtgca aaatcactca gtgctacgac
      961 ggcggtgatc ataccatcgt ggtcggcgaa atcgtcaacg ccaacgccac gggcgaacgg
     1021 ccactgcttt ttttcaaagg gaagtaccag cggctgcctg attcatagcg tccaaaaaat
     1081 caagctcttc gatcgactct tgaaaacact cgaacgcgat catgcgcgga gcgcatagag
     1141 ccgagatcca actttggatc cggttgggtg ttccccctag ggtcaacagg ttcgcccttt
     1201 gtagtgcacc ggcacgctgt aatgtgctct tgcgtcgtgt tcgttccctc cgctccacga
     1261 acacgcatca cggtaacggt tttctatcgc cggcatcttt gctatcggtg gacaatttaa
     1321 tgcattcagg tttagaaatg actctatcaa cgatcaataa gtcgaaacta gcagttcgct
     1381 aaaaaatctg gatgcaggct gctcaaaaga cctcagaggc caggcgcacg ttctaaaggt
     1441 tgatgatgaa ggattaattt gaaaacaaac ccgctccgga agcctcgttt catctttccg
     1501 ctttcatcct tccgttttgc ctgcatgagt cttttcagca gcctgccagt tctcggaggt
     1561 aaggaatgac ccaggtcgta gggagaccaa caccgcgagt cgagggtgag ttaaaggtta
     1621 ctgggaaagc gctttacagc gctgatgtgg agctgccgga tatactctgg ggcaaggtct
     1681 tgcgcagccc gatttcgtcc gggcgaatta agaatatcat gaccgacaag gcgcgccagc
     1741 tgccgggcgt taaagcggtg attaccggag aagatgtggc tggattgaga atcggccggt
     1801 gcatctatga tcacccggtc ctcgcggatg ggctggtgcg attcatcggt gaaaaagttg
     1861 ctgccgtcgc cgccgagacc agacttgcgg cggagcaggc cctcgagctt atcgaagttg
     1921 aatacgatga aatggaaccc ttgctcgatc cggttgcagc aataaaggat gacgcgccga
     1981 ttctccatcc ggatctgctt tcctataagg gccttcccgt gccgctggaa aaggtcagca
     2041 acgtgtttgc ctatttgaaa tggggcaagg gcgatatcga agccggcttc aggcaagccg
     2101 aggtgatcgt cgaaaacacc tttacaaccc aggtcactca tcagtcttat ctcgagccgc
     2161 atgcttgcgt tgtcagggtc gatccgagcg gcggcgcgag tatttggtcg tgcagcaaga
     2221 c
//
LOCUS       DASUTG010000046         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_303186, whole genome shotgun sequence.
ACCESSION   DASUTG010000046 DASUTG010000000
VERSION     DASUTG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303186"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /locus_tag="VF089_00990"
     CDS             complement(<1..2348)
                     /locus_tag="VF089_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6172558.1"
                     /translation="MAERGIDPQEEKKSYGSVFLVGIALLVALSIWAFWDDNITRRPW
                     KSFQSQFFRLDYNKAKAAYEEENKKLQADPAYQELAKKLAAFQASIEDGELGEKLATL
                     ERQETEATVRFGEIDQEVKFIKSELEEAWYEHDHAIQQRRDPKPYAQHIQELEKEKAK
                     LDPQLESARQKRDHLREEIKKINSINKEIQTELSKLTAERDRWRRVMENTTFNVGPFS
                     FYKIPKIQQTVMEEFDRNRFDQPIARVDRCQTCHLAINRAGFENEPHPFKTHPRREVL
                     LADSAHPPEKFGCTGCHEGQGVMVNGVKQAHGEVHLWEFPLLRGAKSQSSCTSCHLDV
                     QKLQGVPLLTEGQRLFEQVGCTGCHLVQGYEDIPKIAPSLTKISAKVDPSWMVRWVEN
                     PHNFRPRTRMPNFEFKPEEALAISAYIWSQSKEEAENWLQQNPLPAGIREADANEAAR
                     GKKLVESIGCKGCHGFAEGEFSTPLGKTKDLVPNLKDIAAKTGPQWMFHWIKNPKGFE
                     PETKMPSLRLSDEEARSITTYLTTLGTKGETIAGIEEKLADPQNIKNGEALVRKYGCA
                     GCHDIKGMEKESRIGVELTTFGSKTVEELAFGNRTDVRHTWDDWTFHKIKTPRGYATE
                     RVEQLMPQFDLADEDINSLRVLLGGFRERKVGQRYLADQSQRVIDVVEGRRLLQQYNC
                     VGCHEIENRGGFVKKYYGENTAAAPPQLNGEGEKVQSQWLFGFLKAPFTLRPWLDIRM
                     PTFGLSDQHTAQLIKYFNGLSRVENPFAYFDERNVPPDHLDAA"
     gene            complement(2358..2525)
                     /locus_tag="VF089_00995"
     CDS             complement(2358..2525)
                     /locus_tag="VF089_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172559.1"
                     /translation="MFYVAKILQALGLADVGYALLTGIMKNSSMAEEIVLALIGLGVF
                     SLGRALERKVA"
     gene            complement(2525..3292)
                     /locus_tag="VF089_01000"
     CDS             complement(2525..3292)
                     /locus_tag="VF089_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6172560.1"
                     /translation="MADAVRERRLPVDDKIHTWPHLVRMEFLVALFVIVALTLWAMMV
                     DAPLEEPANPTRTPNPSKAPWYFLGLQEMLVYFDPWHAGVVLPTLIIVGLMIIPYIDI
                     NPKGNGYYCFKDRKYEILTFFLGFHILWIVTIIIGTFFRGPGWNLFWPWQHWDAHKVV
                     ALTNVDLPYLFGFRDYWWSAIFGLLVIAAYFVFGLVGFYYWVLKVKGREFLERWGMVR
                     FTLTAVIFVLMLSLPAKMFLRLVFNVKYILVTPWINV"
     gene            complement(3316..>3737)
                     /locus_tag="VF089_01005"
     CDS             complement(3316..>3737)
                     /locus_tag="VF089_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX6172561.1"
                     /translation="AMIVFVWLHMFRVFLTGSYKPPREFNWIVGVLLLVLTLLLSFTG
                     YLLPWDQLAIWAVTVGSNMARATPLLGYEGPFAEFVGANPVYDARAFLFGGGEIGPHT
                     LLRFYILHCIFIPLVASILMAVHFWRIRRDGFSGPSL"
BASE COUNT          863 a         1043 c          947 g          884 t
ORIGIN      
        1 gccgcatcta aatggtcggg tggaacattt cgttcatcga agtacgcgaa cgggttttcg
       61 actcttgaga gcccgttaaa gtacttgatc aactgcgccg tatgctggtc cgacaatcca
      121 aaggtcggca tcctaatgtc caaccacggc ctcaacgtaa acggcgcctt gagaaagccg
      181 aatagccact gcgattggac cttctcgcct tcgccattaa gctgcggtgg agccgccgcc
      241 gtgttttcgc cgtagtattt cttaacaaac ccgcctcggt tttcaatctc gtggcaaccg
      301 acgcagttat actgctgcaa caatcttcgt ccttcaacga catcgatcac gcgctggctt
      361 tgatcggcca gataccgttg cccgactttt ctttctctga acccacccag caacacacgt
      421 aacgagttga tgtcttcgtc ggcaaggtcg aattgcggca tgagttgttc gacccgctca
      481 gtggcgtagc cgcgcggcgt ttttatttta tgaaacgtcc aatcatccca ggtatggcga
      541 acatcggtgc gattaccgaa agccaactct tccactgttt tcgaaccgaa ggtggtcaat
      601 tcgacaccga tgcgggactc cttctccatg cctttgatat cgtggcagcc ggcgcagccg
      661 tattttcgca ccaaggcttc gccatttttt atgttctggg gatcggcaag tttttcttcg
      721 ataccggcga tggtttcacc ctttgtacca agggtcgtca agtaggtcgt aatcgagcgc
      781 gcttcctcat cgctgagccg gaggctgggc atcttcgtct caggctcaaa gcccttcgga
      841 ttcttgatcc aatgaaacat ccactgcggc cctgtcttgg cagcaatatc cttgagattt
      901 ggaacaagat ctttagtttt ccccagcgga gtgctgaact cgccttcggc aaaaccgtgg
      961 cagcccttgc acccgatgga ttcgacgagc ttcttgccac gggccgcctc attggcatcg
     1021 gcctcgcgaa tacccgcagg gagcggattc tgctgtagcc agttttcggc ctcttctttg
     1081 gattgcgacc agatataagc cgagatcgcc agcgcttctt cgggtttgaa ctcaaaattc
     1141 ggcatgcgcg tgcgcggtcg gaaattgtgc gggttttcga cccagcgcac catccaactc
     1201 gggtcgacct tggcgctgat ctttgtcaag ctcggcgcga tcttcggaat atcttcgtat
     1261 ccttgaacca gatgacagcc ggtgcagccg acttgttcaa ataaacgttg cccttccgta
     1321 aggagcggga caccttgaag cttttgcaca tcgagatggc atgacgtgca gcttgactgg
     1381 cttttcgcgc cgcgcaacaa cgggaattcc caaagatgaa cctctccatg cgcttgcttg
     1441 acgccgttca ccatgacgcc ctgcccttca tggcaaccgg tgcaaccgaa cttctcaggc
     1501 ggatgggcgc tatcggccaa taagacctcg cgtcgcgggt gcgttttgaa tggatggggc
     1561 tcgttttcga acccagcgcg gttgatcgcc aaatggcagg tttgacagcg gtcgacccgt
     1621 gcaatcggct gatcgaagcg attgcggtcg aattcctcca tcacggtttg ttgaattttt
     1681 ggaattttgt aaaaggaaaa tggaccgacg ttgaaggtcg tgttctccat cacccgtcgc
     1741 catcgatcgc gctcggcggt taacttgctg agttcagtct gaatctcctt gttgatcgaa
     1801 ttgatcttct tgatctcttc tctgaggtga tcgcgctttt ggcgcgcgga ttccaactgc
     1861 ggatcgagtt tcgctttctc tttttcaagc tcctgaatat gttgcgcgta aggcttgggg
     1921 tctcgtcttt gctgaatcgc gtgatcgtgt tcgtaccacg cttcttcgag ctcgcttttg
     1981 atgaatttca cctcctgatc gatttctccg aatcgaacag tggcttcggt ttcctgcctt
     2041 tccaaggttg caagcttttc gcccaactcg ccgtcctcga tgctggcttg gaaagcggct
     2101 aatttcttgg ctagctcctg ataggcgggg tcggcctgaa gcttcttgtt ttcttcctca
     2161 tacgccgctt tggccttgtt gtaatcgagc cggaaaaact gcgactgaaa cgacttccac
     2221 ggccggcgcg tgatattgtc gtcccaaaac gcccagatcg acaacgccac gagtagggct
     2281 atgccgacta agaagacgct gccgtaagat ttcttttctt cctgtgggtc gattcctcgt
     2341 tccgccatcg caactcctca agcgaccttg cgctctagcg cgcggcccaa agaaaaaacc
     2401 ccaagaccga tcaatgccaa aacgatttct tcagccatcg atgaattctt cataataccc
     2461 gtaagcaagg catagcccac gtctgccagc cccaatgctt gcagaatctt ggcgacataa
     2521 aacatcaaac gttaatccag ggggtaacta gaatatattt tacgttgaat actaaccgca
     2581 gaaacatctt tgcaggaagc gaaagcatca ggacgaatat gaccgcggtc agggtaaacc
     2641 gcaccatgcc ccagcgctcg agaaactctc ttcctttcac tttgagaacc cagtagtaga
     2701 agccaaccaa gccgaagacg aagtaggctg caatgaccag caaaccgaaa atcgccgacc
     2761 accagtagtc gcgaaagccg aaaagataag gcaggtccac attggttaag gcgacgacct
     2821 tgtgcgcatc ccagtgttgc cagggccaga ataagttcca ccccgggccg cggaaaaacg
     2881 tgccgatgat gatggtgacg atccacaata tgtggaaccc caaaaaaaac gtcaggattt
     2941 catatttgcg atctttgaaa cagtaatagc cgttcccttt gggattgatg tcgatatagg
     3001 ggatgatcat caggccgacg atgattaacg tcggcagcac caccccggca tgccagggat
     3061 cgaaatagac cagcatctcc tgcagaccga gaaaatacca cggggctttt gatggattgg
     3121 gcgtccgggt cgggttagcc ggctcttcaa ggggcgcatc gaccatcatc gcccacagag
     3181 ttaacgcaac gatgacaaac agtgcgacca gaaactccat gcgcacgaga tgcggccagg
     3241 tatggatctt atcgtcaacc ggaagtcttc tctctcgaac ggcgtctgcc attgtattcc
     3301 tcttacgtga attgcttaca aggaaggacc cgagaaaccg tcgcggcgaa tgcgccagaa
     3361 atgaaccgcc atcaagatgc tggcgaccaa aggaatgaaa atgcagtgca ggatgtaaaa
     3421 tctcagcaag gtgtggggac cgatctcgcc gccaccgaat aggaacgccc gcgcatcgta
     3481 aaccggattg gcgccgacaa actcagcaaa tggaccttca tagcctaata gcggcgtcgc
     3541 ccgtgccatg tttgaaccta cggtgaccgc ccagatcgca agctggtccc acggcagcag
     3601 atagccggtg aagctcagca agagcgtcag caccagcagc aacacaccca cgatccagtt
     3661 aaactcccgc ggcggtttgt aagagccggt caggaatacg cgaaacatat gcagccagac
     3721 gaatacgatc atggcgt
//
LOCUS       DASUTG010000047         6284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_599900, whole genome shotgun sequence.
ACCESSION   DASUTG010000047 DASUTG010000000
VERSION     DASUTG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599900"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VF089_01010"
     CDS             <1..970
                     /locus_tag="VF089_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX6172562.1"
                     /translation="RSVGLRADMDALPIHEANTFAHRSTSDGRMHACGHDGHTTMLLG
                     AAKYLAATRHFDGVVNFIFQPAEEGIGGARAMIADGLFEKFPCDVVFGMHNRPGLALG
                     KFAVRAGPMMAGGGFFDIRITGKGSHGARPESGVDPVLVAAHIVIALQSIVSRNVRPI
                     DAAVVSVTKINGGDAYNVIPQTAQLSGTVRAFTREVMSLIETGMRRVVQGTAEAFGAT
                     AEVDFRVIFAPTVNDVAQANFVAGVCAEVVGEENVSRNPALIVASEDFSFMLEKVPGC
                     YFNIGNGAGENACEVHNPAYDFNDAALALGASVFARIVEKRLAKGD"
     gene            1317..1550
                     /locus_tag="VF089_01015"
     CDS             1317..1550
                     /locus_tag="VF089_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX6172563.1"
                     /translation="MQVAKWGNSLAVRLPSVVVEALNLKEGDHIEIEVAGPRRFEIKK
                     APGARELLARLRKYRGRLPADFKFYRFEANARR"
     gene            1648..1959
                     /locus_tag="VF089_01020"
     CDS             1648..1959
                     /locus_tag="VF089_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX6172564.1"
                     /translation="MVPSAFKWSNEFAAVASRKLSLSWGEIKDILGPIRAICEIDPIS
                     IETHDGAIEIAGRYGFSFYDSAILASALHSGCRVLYSEDFQHGQLIANQLRIRNPFLT
                     A"
     gene            2849..3928
                     /locus_tag="VF089_01025"
     CDS             2849..3928
                     /locus_tag="VF089_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX6172565.1"
                     /translation="MVPEEFLLLAAGVILLIAGAELLVRGASRLAAIAGISPLVIGLT
                     VVAYGTSSPELAVSLKSAFAGQADIAIANLVGSNIFNVLLILGLCAAILPLTVSHQLI
                     WLDVPIMIGASVLAFAVALDGGIGRYDGVMLFAGAVLYTVFALRQGRDIGAMATDDFN
                     REYSDEIKRRPTRWPLQAGFVLAGLAMLVYGARWLVDGAVAAAAALGVSELVIGLTIV
                     AAGTSMPEVATSLIASLRGERDIAVGNAIGSCIFNLLAVLGLCSLIAPDGIEVAPALL
                     TFDMPVMLAVSLACAPIFFTGHTIARWEGALFLLYYAAYTAYVALNAQAHDALPAFSA
                     VMLQFVIPLTILTLAIGYVRHRPRQ"
     gene            4774..4986
                     /locus_tag="VF089_01030"
     CDS             4774..4986
                     /locus_tag="VF089_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172566.1"
                     /translation="METHRKKKNCGVAVTMEGYNGHAWLGQLIRQRGYRLYNLNNLKL
                     ARFKEIFSGAAKNDRIDAPRSWSWFS"
     gene            complement(5210..6100)
                     /locus_tag="VF089_01035"
     CDS             complement(5210..6100)
                     /locus_tag="VF089_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6172567.1"
                     /translation="MTSHNFLQTYQWNYRPQHPRRPDWFKEWPGGAKMAVMLILLHEW
                     ESVPEPFRPMPKGAHHTFDFLALGAREYGARFGVYRLLDVLDRHSVKATVISSGLVAE
                     LFPESVREVRARGQELATHGWDQSMHPPVFKTKEEERDTIVKSTTALERAAGERIVGY
                     MSQGPRPTPNTLEIITELGFVWTADYSDSDVPYLIDVNGKKIVSVGYVMPNFTDNTLV
                     PLGLDGGLKQLQDAFDASYEESQRHPMKFCYACHVHISGRPGMSQLLDRFIAYAQQRD
                     GVRFYPCIDIANFWLEREGK"
BASE COUNT         1322 a         1712 c         1841 g         1409 t
ORIGIN      
        1 gaggtcggtt ggcctgcgag ctgatatgga cgcgctgccg attcacgagg cgaatacttt
       61 cgcgcaccgc tctacgagcg acgggcgcat gcatgcgtgc gggcatgacg gccatacgac
      121 catgttgctc ggcgcggcga aatatctcgc cgccacgcgc cacttcgacg gcgtggtgaa
      181 cttcatcttc cagccagccg aggaaggcat cggcggggcg cgcgcgatga tcgcagacgg
      241 actgttcgag aaattcccct gtgacgtagt gttcggcatg cacaaccggc cggggctggc
      301 gctcggcaag ttcgcggtgc gcgccggccc gatgatggcg ggcggcgggt tcttcgacat
      361 tcggattacg ggcaagggct cgcatggcgc acggcccgag agcggggtcg atccggtgct
      421 ggtggccgcg cacatcgtca tcgcgctgca gagcatcgtc tcgcgcaatg tgcgaccgat
      481 cgatgcagcg gtggtttcgg tgaccaagat caacggcggc gacgcctaca acgtgattcc
      541 gcaaaccgca caactctccg gaaccgtgcg cgcgttcacg cgcgaggtca tgtcgctgat
      601 cgaaaccggc atgcgccggg tggtgcaggg aactgcggag gcgttcggcg ccacggcgga
      661 ggtcgacttc cgcgtaatct tcgcgcccac ggtgaacgat gtggcgcagg cgaatttcgt
      721 agcgggagtc tgtgcggagg ttgtcgggga ggagaacgtg agccgcaacc ctgcgctgat
      781 cgtggcttcg gaggattttt cctttatgct cgaaaaagtg ccgggctgct acttcaacat
      841 cggcaatggc gcgggcgaga acgcctgcga agtgcacaac ccggcctacg atttcaatga
      901 cgcagcgctc gcactcggcg caagcgtgtt cgctcggatt gtcgagaagc ggttagcgaa
      961 gggcgactaa agtcttttca gatgccaggc cctaaacgac tggaatgctt tgaacgctga
     1021 atttcgtttc atcgctcgtc aacggattcg ggttacggag agacgcggca agggagttgg
     1081 ggagtaccgc tccgtgagct tcacatcagt taaatctaag atagtcgcgc caatggcggt
     1141 gactgatact taaagacttg agaagcagcc gaatgtgccg tcgcaatgtg cgatgccgat
     1201 cctatgcgga gtcgctaccg cgggggagaa cggcaagtgc tcccccgcgg ctattgccga
     1261 caggtcgggt gtaagatata cataatgctg tgtatatctt ttacttgggg gtgtgtatgc
     1321 aggtcgcaaa gtggggtaat tctctggcgg ttcgattacc ttctgtggtg gtggaagcat
     1381 tgaatctgaa agaaggagac cacatcgaaa tcgaggtcgc gggcccacgc cgtttcgaga
     1441 taaaaaaagc gcctggtgct cgggaattat tggcgcgcct gcgaaagtat cgcggtcgcc
     1501 tacccgccga ttttaaattt taccgctttg aggcaaatgc ccggcgttaa gatatttttc
     1561 gacaccaacg ttttgctcta tttgctttcc gaggacgcgg ccaaagccga tcgggtcgaa
     1621 gaactgctga caggcggctg acaggcggtg gtaccatcag cgttcaagtg gtcaaacgag
     1681 tttgccgcag tcgcctcgcg aaagttaagc ctgtcatggg gcgagattaa agatattctc
     1741 ggcccgattc gcgccatttg cgagatcgat ccgatttcta tcgagacgca cgacggtgca
     1801 attgaaattg ccggtcgcta tggattctca ttctacgatt cagcgattct ggcgtcggca
     1861 ttgcactccg gatgcagagt tctgtattcg gaagatttcc agcacgggca gctcatagcc
     1921 aatcagcttc ggatcagaaa ccccttcttg actgcttgaa gatggtagtg tcagaaccct
     1981 gacagtcccg cgagcgactg gcgcatgcga ggtgcacaac ccggcctagg atttcaacgt
     2041 cgcggcgctc gcgctcgccg caagccgtgt tcgctcgaat tgtcgagaag cggttaacga
     2101 agggcggcta gaggcttttc aggtgccaga tcttaggacg cgtgaaggtc cagatgccgt
     2161 aggtcgagaa cgtcagtcgg accagaataa ggccatgggg tcctgtgtga attgtgacaa
     2221 acagctcgca accacgaacg tccagccaca gcgcccgtgc gcgaggaccg ttcgcactac
     2281 cgcatcgcat tcatcttcga atgacggcaa cgtgattgaa ctgatgcaga tctgtcccga
     2341 gtcggcgatc tattgggagt gaagaacgaa cttttgggtg gcaacaccgc gctaaaaata
     2401 tccgccaaac gagctatgaa tgtcaaaagt gaagaagcga ccgggcccac cacgaccaca
     2461 aacccacaaa gaactgatct cgactggtcc gggcctcacc gtcactcgca tcatgtttga
     2521 ttcggaattc taactgttag acatgaactt gcggaccagc tcaccaggtt gccagtcgat
     2581 gcaaggaccg tcgtaatcga gaagtctcaa aaatatttga tcgaggaagc cgaccataaa
     2641 cgcatattca agtctacact attccacaat cagccgaata ctcgataaga ggattcatcg
     2701 agcaagaata aagacctgac gcttgatgaa ccctttcgcc caaggttagt cttctggcct
     2761 gaggcacgcc gggcgctatt tcttaaaccg gctgccactt aaaacgttcg tcagtggatg
     2821 tccgattaga cgatcgcgtg caattttgat ggtaccggaa gagtttttac tgctggcagc
     2881 gggcgtgatt cttttaattg caggagcgga gctgctggtg cgcggagcct cccggctggc
     2941 cgccatagcc gggatctcac cgctggtgat cggcctgact gttgtcgctt acggaaccag
     3001 ctcacccgag ctcgcggtca gtttgaaatc cgctttcgcc ggccaagccg atattgccat
     3061 cgccaaccta gtgggcagca acatattcaa cgtgcttttg attctgggac tttgcgcggc
     3121 gattcttccc ctgacggtct cgcatcagct gatttggctg gacgtaccga ttatgatcgg
     3181 agcgtccgtg ctcgcatttg cggtcgcttt ggacggcggc attggtcgct atgacggcgt
     3241 gatgctcttc gccggtgcgg tcctttatac cgtctttgcg ctgcgccagg ggcgcgacat
     3301 cggagcaatg gcgaccgatg acttcaaccg ggaatacagc gatgaaatca aacgccgacc
     3361 tacacgctgg ccgcttcagg cgggctttgt ccttgcgggt ttggccatgc tcgtctacgg
     3421 cgcgcgctgg ctcgtcgatg gcgcagttgc cgcagccgcc gcgctgggcg taagcgagct
     3481 tgtcatcggc ctgacaatcg tggccgcggg gacgtctatg cccgaagtcg ccacctccct
     3541 tatcgccagc ctgcgcggcg agcgcgacat tgcagtggga aatgccatcg gcagctgtat
     3601 cttcaatctg cttgcggttc tcggattgtg cagtctgatt gcccctgatg gaatcgaagt
     3661 cgcgccggcg ctcctgacct tcgatatgcc ggtaatgctc gctgtctcgc tggcatgcgc
     3721 gccgatcttt tttaccggcc acaccatagc tcgctgggag ggcgcgcttt ttctgctcta
     3781 ttacgctgcc tacaccgcct atgtagcgct caatgcccaa gcgcacgatg ccttgccggc
     3841 cttcagcgcc gttatgctgc agttcgtaat tccattgacg attttgactc tcgccatcgg
     3901 ctacgtacgt catcggccgc gccagtaatg caactcacgt agcctgcatc gtgacatcgt
     3961 cgattcgccg cgagcgattg gcgaatgcga ggtgcacaac ctggccagga tttcaacgac
     4021 gcggccctcg cactcgacgc aagcgtgttc gctcgaattg tcgagaagcg gttagcgaat
     4081 ggcggctaga cctttcaggt gccagatctt aagacgcgtg aaggtccgga tgccttaggt
     4141 cgatagcgtc agtccgatca gaatgaggcc ataaatccgg cgtgaattgt gacaaagaac
     4201 tcgcaaccat gaacgtccag cacggcgccc gctagcgaag accgctagca ctaccgcatc
     4261 gcgttcatcc tcgaccccga cggagcccct gactcgaaaa agtagcactg ttccctttat
     4321 tgttagtaag ctttgtactc gtcggaaacg cccgggcgct ggggctgcag gctccaaaga
     4381 cgcattggac caacctcgtt cgtacgtggc ttctatttct cctatgctac tcagagtcgg
     4441 tttttctttc atatcatctg aaatccggcg ccgctcgaat tcattaagct ccatcttcga
     4501 gtcaccgaag cagaaacgag caaaatgtat caatgcccga cctgattgaa tgaagagctg
     4561 caccttgcag ctttgggtgg taacggttct ctatggacca attataagag gctggctcaa
     4621 tgaagagccg aataaacgca aaggaggtgc agcatgagag tatataggag ttgatttagg
     4681 ctttggtgag gtgatgggac tgtcgagcgg cgaggtgctt gaacagttcg agatcgatca
     4741 ccggatcgag ggattcgaga agttctttct cggatcgaga cacaccgcaa gaaaaagaac
     4801 tgtggcgtag cggtaacgat ggaggggtac aatggccatg cctggcttgg acagctgata
     4861 cgccagcggg gctaccgact ttataatctg aacaacttaa agcttgcccg ttttaaggag
     4921 attttttccg gagccgccaa gaacgaccgg atcgatgcgc caaggagttg gagttggttc
     4981 agctaagcga tcatcggggg gtgaagttcg ggatcagctt ctacgactcg ttatttcttg
     5041 caatcgatcc attctttcct ccgcttcgta tccgattgca tttggccacg atgcccatgt
     5101 tccggtctta atcccaggat caaatcatgg cttaagtatt cacgagatct tgaaaccacc
     5161 ttggacctgc ggttcaggcc cgatcttttc ccggatgtgc gtaccttgtc tattttcctt
     5221 ctcgttcgag ccagaagttt gcgatatcga tgcaggggta gaacctcact ccgtcgcgct
     5281 gttgcgcata ggcgatgaag cgatcgagca gttgactcat tccgggtctg ccgctgatgt
     5341 gcacgtggca ggcatagcag aacttcatgg gatggcgctg agactcctcg taactggcgt
     5401 cgaaggcgtc ctgcagttgc ttcagaccgc cgtcgagacc gagcggcacg agcgtgttgt
     5461 cggtgaaatt cggcattaca tagcccaccg agacgatctt tttcccattc acgtcaatga
     5521 ggtatgggac gtccgagtcg gaatagtcgg cggtccacac aaaccccagc tccgtaatga
     5581 tctcgagtgt gttgggcgtt ggccgcggtc cctgcgacat gtagccgacg attcgctcac
     5641 cggcggcgcg ttcgagcgca gtcgtggact tgacaatggt gtcccgttcc tcctccttgg
     5701 tcttgaacac cggcgggtgc atcgactggt cccagccgtg ggtggcgagc tcttggccgc
     5761 gcgctctgac ttcgcgcacg ctttccggga acagctccgc gaccaaaccg ctgctgatca
     5821 cagtcgcttt gacactgtgg cgatctaaaa cgtccaggag gcgatagacc ccgaacctgg
     5881 cgccatattc gcgcgcgccc agcgccagga agtcgaacgt atggtgcgcg ccttttggca
     5941 taggcctgaa gggctcgggc actgactccc attcgtgcag caaaataagc atcaccgcca
     6001 ttttggcgcc gcctggccac tccttgaacc agtcggggcg cctgggatgc tgcggccggt
     6061 aattccattg gtacgtctgg aggaaattgt gtgaagtcat ggtcttgata ctcctttgct
     6121 gtttcggtct tgcacggagc tactctagcc atcagtgatg catcggagtc ttccatcgga
     6181 atctagcagg cagcgggagc gaaagaaacg agtattaact tcctgacgag ggtgtggcta
     6241 agaatgaaac ctaaccaagg ctgttcattg ccgaggcggg tggc
//
LOCUS       DASUTG010000048         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_748018, whole genome shotgun sequence.
ACCESSION   DASUTG010000048 DASUTG010000000
VERSION     DASUTG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748018"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VF089_01040"
     CDS             complement(<1..1216)
                     /locus_tag="VF089_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX6172568.1"
                     /translation="MSKRPNFLLIITDQQRADHLGCYGNSVVQSPNIDGLAAAGTRFD
                     RFYVATPICMPNRATLMTGRMPSLHGARQNGVPLSLTATTFVDILHAAGYDTVLFGKC
                     HLQSINGTPPVIGMPQPNSNRVQPPEYLREADKSFNEHGRYDQELPSTWERDPEFEPT
                     IPYYGFNYVELAIGHGDKAVGHYERWLKVRHSDSDSLRGAKNQLPGNDYIAPQAWRTR
                     MPEELYPTTFVAQRTIDYLETYSRSDQSQPFFIQCSFPDPHHPFTPPGKYWEMYDPRS
                     ISLPSSFNSTERPIPPHLQKLYEERELKKSNRDGQRTFAISEREAREAIALTYGMITM
                     IDDAIGKILARVVALGLESNTVIIFTTDHGDFMGDHQLLLKGALHYRGLVRVPFIWRD
                     PAALKKNIVNSGL"
     gene            complement(2002..>2282)
                     /locus_tag="VF089_01045"
     CDS             complement(2002..>2282)
                     /locus_tag="VF089_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172569.1"
                     /translation="EVPTKFDLVINLKTAKQIGLAIPQNALARPESYQVGDSMAELDK
                     DDVVNRSHREAGVCYLGPLITDVGEGLCLSCLRYRRAFASWHMWSLLF"
BASE COUNT          554 a          583 c          593 g          552 t
ORIGIN      
        1 ataggccgga attgacaatg ttctttttca gcgcggcagg atcacgccag ataaagggca
       61 cccgaaccaa cccccggtag tgcagcgccc ccttcagaag tagctggtga tcgcccatga
      121 agtcgccatg atcggtagta aagatgatca cggtgtttga ctccagcccc agcgccacaa
      181 cccgcgcgag tatcttgccg attgcatcat cgatcatcgt gatcatgccg taagtaaggg
      241 cgattgcctc gcgtgcctcg cgctcgctga ttgcgaaagt ccgctgtccg tctcgattgc
      301 tcttcttcaa ctctcgttct tcgtagagtt tctgtaggtg cggtgggata ggtctttctg
      361 ttgaattgaa cgacgacggc aatgaaatcg atcgaggatc atacatctcc cagtacttgc
      421 ccggaggtgt gaatggatgg tgtggatcgg ggaacgaaca ctgaatgaaa aacggctgac
      481 tctgatctga acgagaatat gtctccaaat aatcaatggt gcgttgcgcg acaaaagtgg
      541 ttggatagag ttcttccggc attctcgtgc gccaagcctg cggtgcaata taatcgtttc
      601 cgggaagctg gttcttggct ccacgcaagc tgtcggaatc cgaatgacgg actttgagcc
      661 accgttcata gtgtccgacc gccttgtcgc cgtgcccgat agccaattcc acatagttga
      721 aaccgtaata ggggatcgtc ggttcaaatt caggatctcg ctcccatgtc gatgggagtt
      781 cctgatcgta gcggccatgt tcgttgaaag acttatcggc ttcgcgcaaa tactctggcg
      841 gctggactcg gttcgagttc ggttgcggca tgcctatgac cggaggagtg ccgtttatgc
      901 tctggagatg acattttcca aagagaaccg tgtcgtagcc ggcagcgtgc agaatgtcga
      961 caaaggtcgt ggccgttagt gacagtggaa caccattttg ccgggcaccg tggagcgacg
     1021 gcatgcgacc agtcataagc gtggctcggt tcggcatgca aatcggcgtt gccacgtaaa
     1081 agcgatcgaa ccgtgtgcct gccgccgcca acccatcgat gttgggactc tgcaccacgg
     1141 aattgccata acaacccagg tgatcggctc gttgctggtc ggtgatgatc aagagaaaat
     1201 tggggcgctt actcatcggc gttaccgtcc ttccatggct gttatcacac aagaaaactc
     1261 cctcgaaaac ccttctacaa cgaaatgcat ccaagcggaa gaatcagtca aaaccgatgt
     1321 cgccgtatca gggtcggatc tgctagagcc gcaagcaatg ccacaccatt agagtacaga
     1381 gtggatttgc tccatctatt ctgtgcggtt tagctttgtt caagcttgga actgggtaga
     1441 agtagtgttg tcggatgacg gatggcgaag aatcgtaggt ttccaatgat cccgcgacta
     1501 taaacaaatc ggaatgataa tcatccgctc ccagcaattt tctctcgggt aaaatggcgt
     1561 atgaataacg atgcagatta gcttgcagcg ctgctgcgag ggtcgcctgg attacgaaag
     1621 atggttaccg gtttgcgaaa tcgattgttt ttattcgctt ctaacacttg ttaggagagc
     1681 atgatattta aatggaagag gcaaatcatt catcattggc gtccccgacg ggatttgaac
     1741 ccaagttgat caaaaaatcc ggcaatggaa agcgctagta tcatcgggag atacagcgct
     1801 tttcgtgttt atggcacttg ctcatccatc ccgaaatatc cctcaatcca cccgctaaat
     1861 cccgcataaa ccgaaacggt ttcggttcga tttcctttac gttcctgaag acctgagggg
     1921 gtgttggctc atctccccct agggtatctg gcttagggga ggtagctcga cacgccgcaa
     1981 aaacaaaaag gctttccgca actagaaaag gagactccac atatgccacg aggcaaacgc
     2041 acgtctatac ctcaagcagc tcaagcaaag gccctcgccg acatcggtta tcaagggacc
     2101 caaatagcag acgccggcct cccggtgcga acgattgacg acatcatctt tgtctagttc
     2161 tgccattgag tctcccactt gataactctc cggtcgcgcc agcgcatttt gcgggatcgc
     2221 caaaccaatc tgcttcgctg tcttgaggtt aatgaccaaa tcgaacttcg ttggtacttc
     2281 aa
//
LOCUS       DASUTG010000049         5829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2082583, whole genome shotgun sequence.
ACCESSION   DASUTG010000049 DASUTG010000000
VERSION     DASUTG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082583"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1190)
                     /gene="dnaJ"
                     /locus_tag="VF089_01050"
     CDS             complement(78..1190)
                     /gene="dnaJ"
                     /locus_tag="VF089_01050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6172570.1"
                     /translation="MNGKKDYYELLGIARNAGEEEIKKAYRKLALQHHPDRNPGDKQA
                     EETFKLISEAYQVLSDPQKRAQYDQYGHAAFGDNGPFAGGFDFNSGFEDIFGDIFGEF
                     FGGAGGRRRRQTRGEDLRYNLSLSFEEAVAGVEKKIKIPRHGPCETCRGSGAKPGTSP
                     QTCPTCHGRGQVSFQQGFFSVSRSCNQCHGQGTVIADPCGSCGGVGRTRKMHTLSVKI
                     PAGVDNGSRLKLRGEGESAPSGGAPGDLYVVIQVETHPIFTRENLDILCDVPITFVQA
                     ALGAEIDVPTLDGKVKMKVPAGTQSGKLFRIKGKGVKDVHGYQQGDQHVRVLVETPTH
                     LTARQKELLKEFASLGGEDVNPIAKGFFDKVKQLFD"
     gene            complement(1308..3224)
                     /gene="dnaK"
                     /locus_tag="VF089_01055"
     CDS             complement(1308..3224)
                     /gene="dnaK"
                     /locus_tag="VF089_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6172571.1"
                     /translation="MAKVIGIDLGTTNSCVAILEGGDPNVLANSEGSRTTPSVVAFTD
                     SGERLAGQIARRQAITNPENTIFAVKRLIGRRFDDPMVQRAAKVLPYKVVRNDNGDAW
                     VEIRGKQYSPAEISAFILQKMKQTAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLNVLRIINEPTAASLAYGLDKKKDEKIAVFDLGGGTFDVSVLELGDGVFEVKATNG
                     DTFLGGEDFDQRIIDYLADEFKKDQGIDLRKDRMALQRLKEAGEKAKCELSSSMETDI
                     NLPFITADQQGPKHLNMKLTRSKLEALCGDLLDRLEAPCRQALKDAGLSASDIHEVVL
                     VGGMTRMPAVQERVKKIFGKEPNKGVNPDEVVAVGAAIQGAVLTGDVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAQDNQTAVSIRVFQGEREMAADNKLLG
                     QFDLVGIPMAPRGVPQVEVTFDIDANGIVHVAAKDLGTGKEQSIKITASSGLNEEEIK
                     RMVADADAHASEDKKRKETVEARNHLDTLIYSTEKSLKEHGGDLEPGVKENIESALTQ
                     AKAALEGQDAQAMKTAADQLSQSSHKLAESMYAKASQQQKTQDTGEPEGQSTDDGKKK
                     KEDVVDADFTEVKE"
     gene            complement(3300..3908)
                     /gene="grpE"
                     /locus_tag="VF089_01060"
     CDS             complement(3300..3908)
                     /gene="grpE"
                     /locus_tag="VF089_01060"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6172572.1"
                     /translation="MAEESKKPDHTSDAAEEIVDSPPARQDELAELRDKLQAKEKESE
                     ENYERYLRQVAEVDNFKKRVNREKEDAIRYANENLIKDILPVIDNLERAIAHAQGGGN
                     GKPLVEGVEMVLRGLLDVFSKHGVVQVPAVGAVFDPGKHEAMAQVESSEYEPNTIIDE
                     HHKGYLFRDRLLRPALVTVAKAPQQKDGKNGQSEVEKAPADD"
     gene            4054..4827
                     /gene="recO"
                     /locus_tag="VF089_01065"
     CDS             4054..4827
                     /gene="recO"
                     /locus_tag="VF089_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6172573.1"
                     /translation="MQTSHVTSAIVLRSWPFGESDKIVSFLTEGHGKLTGIAKGAKRS
                     KKRFVNSLEPFSFVSLNFHDRSKGSLAFILRCELQKSYKNLIASLEKIAVASYYVEIT
                     EGLTAEREQDRLVFDHLKRSLNRLENEEVSVTFLIEFELKLLSLTGYQPFLDSCCRCG
                     MHPSFSHAAVQTHWYFSLRDGAILCARCSKLRKELVPISGATLDYMANLQQESNRSFD
                     DPKPSQTILKQIRSATVRFIQFQLNREIKSASFLNQFCF"
     gene            4873..>5829
                     /locus_tag="VF089_01070"
     CDS             4873..>5829
                     /locus_tag="VF089_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX6172574.1"
                     /translation="MPKNNKCVYFFGGGKAEGKASMRELLGGKGAGLHEMTRIGVPVP
                     PGFTITTEVCRYYHARGRYPKSLELDVSQALKRLEKKIGKRFGDPHEPLLVSVRSGAR
                     ESMPGMMDTVLNLGLNDQTLQGLIRQTQDPRFAYDTYRRFVQMYSDVVLGVKPRDKTQ
                     VNVFEQIIEQKKRDRGFRSDIELNGNDLKEIVGRFKNAVKAKTGEDFPDDPVAQLWGA
                     IGAVFRSWNNDRAVAYRELYHIPDDWGTAVNIQAMVFGNLGKACATGVAFTRDPSTGE
                     KKFYGEFLVNAQGEDVVAGVRTPRAIAELKGLMPKPYAQLRRV"
BASE COUNT         1329 a         1543 c         1405 g         1552 t
ORIGIN      
        1 ggaggtcaac aacaatttta ctttccgctg aagcagctgc aagggttact aaagaagttt
       61 tgtctctgga aagcgagcta gtcgaacaac tgttttactt tgtcaaaaaa accttttgct
      121 atcgggttaa cgtcttctcc acctaacgac gcaaactcct tgagaagttc cttctgccgc
      181 gccgtcaggt gggttggcgt ctcaaccaat acacggacgt gttggtcgcc ttgctgataa
      241 ccatggacgt ctttgacgcc cttgccttta attcggaaga gcttacccga ctgcgtgccc
      301 gccgggacct tcattttgac cttgccgtct aaagtcggga catcaatctc cgcgcctaaa
      361 gcggcctgca caaaagttat cgggacgtcg caaagtatat ccaggttctc ccgcgtgaag
      421 atgggatgag tctctacctg aatcacaaca tacaagtctc ccggcgcccc cccgcttggg
      481 gcgctttccc cttcgccgcg gagcttaagc cgagaaccat tgtcaacgcc ggcgggaatc
      541 ttcacactca gcgtgtgcat tttgcgggtc cgcccaactc caccgcaaga cccgcatgga
      601 tcagcgatga cggtcccctg tccatgacat tgattgcagc tccgtgagac gctgaagaat
      661 ccttgttgaa aactgacttg gccgcggcca tgacatgttg gacaagtctg tggtgaggtg
      721 ccaggtttgg cgccgctacc acgacaggtt tcgcagggtc catggcgtgg gatcttgatc
      781 tttttctcga caccggcgac tgcttcttca aagctcaagc tgaggttata acgaaggtct
      841 tctccgcgcg tctgcctacg acggcgtcct ccggcgccgc cgaaaaattc gccgaagata
      901 tcgccgaaga tatcttcaaa gccagaatta aaatcgaatc cgccagcaaa gggaccgtta
      961 tcaccgaaag cagcatgacc gtactgatcg tactgtgctc gcttctgcgg gtccgaaagt
     1021 acctgatagg cctcggaaat cagcttaaac gtttcttccg cctgtttgtc tccggggtta
     1081 cgatcgggat gatgctgaag cgcaagtttt cggtatgctt ttttgatctc ctcctcgccg
     1141 gcattccggg cgatccccag cagctcataa taatcttttt tgccgttcaa gcgacgcctc
     1201 gttttgctaa cgttttgtac acgaaggaaa gcccctctcc gtcgccagtg atcggagcag
     1261 gggctttccc gtcgatttgt taatgaccac tagaccaggt tgtgaaatta ctctttaacc
     1321 tcggtgaagt ccgcgtcgac cacatcctct ttctttttct taccgtcgtc ggtgctttgc
     1381 ccttccggtt cacctgtatc ctgggttttt tgctgctgcg aagctttggc gtacatcgat
     1441 tcggccaatt tgtgagacga ttgactcagc tgatcagccg cggttttcat tgcctgggca
     1501 tcctgacctt ctaaggcggc cttggcttgc gttagtgcgg actcgatatt ttccttcact
     1561 cctggctcga gatcgccgcc gtgctctttg agcgactttt cagtagagta gatcaacgta
     1621 tcaaggtgat tgcgcgcctc aacagtctct ttccgttttt tatcctctga cgcatgtgcg
     1681 tcagcgtcgg caaccatccg cttgatttct tcttcgttga ggccgctcga cgcggtaatc
     1741 ttgatcgatt gttcctttcc tgttcctaga tctttcgctg cgacatgaac aataccatta
     1801 gcgtcaatgt cgaaggttac ctcaacttga ggaacaccgc ggggtgccat cggaatgccg
     1861 acaagatcga actgcccgag gagcttgttg tccgcggcca tttcccgttc gccttggaaa
     1921 actctgatag acaccgcggt ttgattatcc tgcgcggtgg aaaacacctg gctctttttg
     1981 gttggaatcg tggtgtttcg ctcaatgagc tttgtgaaaa ccccacccaa cgtttcgatt
     2041 ccaagtgaaa ggggcgttac atctagcagc aagacgtctt taacatcgcc cgtcaatacg
     2101 gctccctgaa tagcggcacc tactgcaacg acctcatctg ggttaacgcc tttattcggc
     2161 tctttaccaa aaatcttttt gacacgctcc tgcactgccg gcatccgagt cattccacct
     2221 actaatacga cctcatggat gtcgctggcg ctcaggccgg cgtctttcag cgcctgccga
     2281 cagggggctt ccagccgatc aagcagatcg ccgcaaagcg cttcgagctt cgaccgagtc
     2341 agcttcatat tgagatgctt cggaccttgc tgatctgctg tgataaacgg caggttaatg
     2401 tcggtctcca tagaagaaga taactcgcat ttggcctttt cacctgcttc ctttaggcgt
     2461 tgcaacgcca tccgatcctt ccttagatcg attccttgat ccttcttaaa ctcatcagct
     2521 aaataatcga tgattctttg gtcgaaatct tcgccgccca gaaaagtatc gccgttggtg
     2581 gctttaacct caaaaacccc atcgcctagc tccaggacgg aaacgtcgaa agtcccaccg
     2641 cccagatcga aaacggcgat tttctcgtct ttcttcttgt ccaagccata agcgagtgac
     2701 gcagcggtgg gttcatttat gatgcgaagg acatttaatc ctgcgatacg tccagcatcc
     2761 ttggttgcct gtctttggct gtcattgaag taggctggga cggtgataac cgcctcggtt
     2821 accttttcgc caaggtaatc ttccgcagtt tgcttcatct tttgcaaaat gaaagcggaa
     2881 atctcggcgg gactgtattg tttgccgcga atctctaccc aggcatctcc attgtcgttg
     2941 cgaacaacct tataaggcag aactttggct gctctttgaa ccataggatc atcgaaacgc
     3001 ctgccgatta gccgcttgac ggcaaaaatc gtgttttcag ggttggtgat agcttgtctt
     3061 cgtgcaattt ggcctgccag gcgctctccg ctatcggtga atgcgacgac tgacggggta
     3121 gtccggcttc cctctgaatt tgccaagacg ttaggatcac caccctccaa aattgcgacg
     3181 caggagttgg ttgtgcctaa gtcaattcca ataacttttg ccatttcagt tgctcctaaa
     3241 tgaagtctaa cgttcttaaa cgctaacgaa gtcttaaccg ttcagaacca aaccttgatt
     3301 taatcatcgg ccggggcctt ttcaacctca ctttgaccat ttttcccgtc tttctgttgc
     3361 ggggctttag caaccgtcac aagcgccgga cgcaggaggc ggtcacgaaa gagataaccc
     3421 ttatgatgtt catctatgat ggtgttaggc tcgtactcac tgctttcaac ctgcgccatc
     3481 gcctcgtgct tacctggatc aaacacagct cctactgccg ggacctgcac cactccgtgt
     3541 ttgctaaaga cgtccagcag tcctcttaac accatttcaa cgccttcaac caacggctta
     3601 ccattgccgc cgccctgtgc atgggcgatc gctctttcga ggttatcgat caccggaaga
     3661 atatctttga tcaggttctc gttagcatag cggatggcat cctctttttc tcgattgact
     3721 ctcttcttga agttgtccac ttcggcaact tgtctgagat aacgctcgta attctcctcc
     3781 gattcctttt ccttggcttg gagtttgtcg cgcagctccg ctagttcatc ttgacgtgct
     3841 ggcggggaat cgacaatctc ttcggccgcg tcactcgtat gatctggttt tttgctttcc
     3901 tcggccattc taagtccccc acaaagtgct ggagcagaat ctaatcacgg gagtggacta
     3961 tgtcaagttt gataaacgta aaaagcttaa acttattaaa tacttgtcga atatcagaaa
     4021 cgatgctaag gtgctgctgg cgcgaataca tccatgcaaa ccagccatgt tacctcagcc
     4081 atcgtcttgc gtagctggcc tttcggagaa tctgacaaga ttgtctcatt cttaactgaa
     4141 ggtcacggca aactcaccgg cattgcgaaa ggagctaagc gttctaagaa gcgcttcgta
     4201 aactccctcg agccgttctc cttcgttagt ctaaactttc acgaccgatc taaaggcagt
     4261 ctagcattta ttctccgctg cgaattgcag aaaagctata aaaatttgat cgctagctta
     4321 gaaaaaattg ccgttgcctc ttactacgtt gagatcacgg agggactcac cgccgagcgc
     4381 gaacaggata gattagtttt tgatcacttg aagagaagcc ttaaccgtct ggaaaatgaa
     4441 gaagtatctg taacttttct cattgagttc gaattaaagc ttcttagttt gaccggctac
     4501 cagccatttc tagatagttg ctgccgctgc ggtatgcatc cctcattctc gcacgcggca
     4561 gtacagacgc actggtattt cagtctccga gatggtgcga tactttgtgc acggtgttcc
     4621 aagcttagaa aagaactcgt gcctatatcc ggtgcaactc tcgactacat ggcaaatctt
     4681 cagcaggaat cgaatcgaag ttttgacgat ccgaagccct cgcaaacgat tctaaaacag
     4741 attcggtctg ctacggtgcg atttatacag tttcagctaa atagggaaat caaatcggct
     4801 tccttcctca atcagttttg tttctgatca ttcctgttta caaacggctg gatacagaac
     4861 gaggttattg cgatgccaaa aaacaataaa tgtgtttact tcttcggcgg tggaaaggct
     4921 gaaggtaagg catcgatgcg cgagttgctc ggcggcaaag gcgctggcct acacgagatg
     4981 acgcgtatcg gagttccggt acccccgggg ttcaccatca ccacggaagt gtgtcggtac
     5041 taccacgctc ggggtcgtta ccctaaaagc ctggaactgg atgtaagcca ggcgcttaag
     5101 cgtctagaga agaaaatcgg caaacgcttt ggagatcctc acgagccgtt gttggtgtca
     5161 gttcgttctg gggcaagaga gtcgatgccg ggtatgatgg ataccgtgtt gaacttgggg
     5221 ctcaatgatc aaaccctgca aggcctcatc cgtcagacac aagacccacg attcgcctat
     5281 gatacttatc ggcgattcgt gcagatgtat tctgacgtcg tgcttggagt caagccgaga
     5341 gataagacgc aagtcaatgt gttcgagcaa atcattgaac aaaagaaacg cgatcggggc
     5401 ttccgctctg acatcgaatt gaacggtaat gacctaaagg agatcgtcgg cagatttaag
     5461 aacgcggtta aggcgaaaac tggtgaggat tttccggatg accccgtcgc acagctttgg
     5521 ggagcgatcg gcgcagtctt caggtcgtgg aataacgatc gagctgttgc ataccgtgag
     5581 ctttatcaca ttccagacga ctggggcacc gcggtcaata ttcaggcaat ggtttttggc
     5641 aacctcggaa aggcttgcgc caccggagtt gcgttcaccc gagatccctc taccggagag
     5701 aagaagttct atggcgaatt cttggtcaat gcacagggcg aagatgtcgt tgcaggtgtt
     5761 cgaacaccta gagccatcgc cgaactaaaa ggtctgatgc caaaacctta tgcacaattg
     5821 agaagagtc
//
LOCUS       DASUTG010000050         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1489532, whole genome shotgun sequence.
ACCESSION   DASUTG010000050 DASUTG010000000
VERSION     DASUTG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489532"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VF089_01075"
     CDS             <1..635
                     /locus_tag="VF089_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX6172575.1"
                     /translation="DNGTDVHLAFVNLDFRPTLPAVDTLIIHTTCSNRDLPGKLPFGG
                     DRGEFELEGAAPLSRIRCLRKPTETLRAPLRRGTQWRIISHLGLNYLSLCEGGLEALR
                     EILKIYDFADSSSVRQQINGITRLTSKPVVGRPRSMPWNGFCRGLEVTVEFDEEKFVG
                     GGVFLFAAVLENFFGLYTSLNSFTQLVAKSRQREEPLKRWPPRAGEQILL"
     gene            599..1717
                     /gene="tssG"
                     /locus_tag="VF089_01080"
     CDS             599..1717
                     /gene="tssG"
                     /locus_tag="VF089_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX6172576.1"
                     /translation="MATQSRRADPSLEHILFEEGYRFDFFQAVRVLERLSSARLPVGR
                     SSIPSKEAVRFRSLLALSFPASSIYDVAQSDNGDSPPEMVVAFMGLFGLAGVLPRHYT
                     ELLLERVRQKDFALRDFLDLFNHRMISLFYRAWEKYRFPVAYERSRSSPGATHDPFSF
                     ALFNLIGMGTDGLRGKLKSGDEPLLYYSGLIAQQPRSASALQAMLGDYFNVPVIVRQF
                     IGAWLHVDNENRTRLGRGDSNNELGSTTMLGSRCWDQQAGFTLAVGPLKFNQFRQFLP
                     SETGFSSLVELTRFFAGLTLDFNVQLVLQAAEVPGCRLTRPAPEALRLGWSSWLKTKE
                     FSRDAQAVLASHLTRMQKLSGHPSQATLKEAGLHERQS"
     gene            1701..4370
                     /gene="tssH"
                     /locus_tag="VF089_01085"
     CDS             1701..4370
                     /gene="tssH"
                     /locus_tag="VF089_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX6172577.1"
                     /translation="MNVNLKSLIGKLNNTGRQALEGAAGLCVTRTHYDIDIEHVMLKL
                     VEAENTDIQKILRHYEVDQTRLSRDLTRGLDRLKTGNARTPAFSPRIPRLITEAWNLA
                     SIDFGATKVRSGHLLLAILGEEDLRRLAREVSPELQKVSVEELHKHFADLTAGSAEDE
                     MASPASAETADPDMRGPTKTPALDQFTIDLTDRARKGQIDPVLGRDFEIRQMVDILTR
                     RRQNNPILTGEAGVGKTAVVEGLALRIAQGDVPAPLKNVALKSLDLTLLQAGAGVKGE
                     FENRLKSVIEEVKSSAQPIILFIDEAHTMIGAGGQAGQGDAANILKPALARGELRTIA
                     ATTWSEYKKYFEKDAALTRRFQVVKVDEPTEEQAVAMLRGLMSTLESHHNVRILDEAM
                     DAAVRLSHRYITGRQLPDKAVSVLDTSSARVALGQMTTPPALEDCQRRIAQLDTEMGI
                     LEREALTGGNTNERIESLAKEKAAIEGRLAELNNRWSEEKQRVEELRGLREKLEAHAA
                     ASKQAGNGETDGKLSPEEVKQVQGEIARLNDELAKIQGESPLMQVCVDAQAVAEVVSG
                     WTGIPVGKVLKDEIQTVLSLKDRLEERIIGQSHALEAIAQRIRTARAKLEDPRRPIGV
                     FMLVGPSGVGKTETALALADTLFGGERNVVVINMSEYQEGHTVSGLKGSPPGYVGYGE
                     GGVLTEAVRRKPYSVVLLDEMEKAHTDVMELFYQVFDKGMLEDGEGREIDFKNTLILM
                     TSNAGTDTIAKLCADPDTKPEPDALADALRPDLLKVFKPAFLGRAIIVPYYPIADNIM
                     KKIIELQLGRIQKRLFENHRAQFSYDPALVDEVARRCTEVESGARNVDHILTRTLLPE
                     ISKEFLAKMAAGEGISRVHVSVGGQGGFQYKIA"
     gene            4436..>4715
                     /locus_tag="VF089_01090"
     CDS             4436..>4715
                     /locus_tag="VF089_01090"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX6172578.1"
                     /translation="MNYTQQDRAMAVTTPLGADKLLLLSFTGQEAISRLFDFQLDLTA
                     ENEVDVAFDKLLGQKITTRLDLPDGEKRYFSGICNRVGQGERDEIFTRY"
BASE COUNT         1033 a         1252 c         1395 g         1035 t
ORIGIN      
        1 gggataacgg caccgacgtg catctggcgt tcgtcaatct tgatttccgg cccacccttc
       61 cggccgtgga cacgttgatc attcacacca catgcagcaa tcgggatctt ccggggaagc
      121 tgccgttcgg aggtgatcgc ggggagttcg agctcgaggg ggcggcgccg ctatcgcgca
      181 tccgttgcct gcgcaagccg accgagactc tgcgcgcgcc gctacgccgc ggaacgcagt
      241 ggcggatcat ctcccatctc ggcctcaatt atttgtcgct ctgtgaggga ggtctcgaag
      301 cgttgcggga aattctaaag atctatgact ttgccgattc ctcctccgtg cgccagcaaa
      361 tcaacggtat tacgcgccta accagcaaac cagtcgtcgg ccggccgcgc tcgatgccat
      421 ggaatggttt ctgtcgcggg ctcgaagtca cggtcgagtt cgatgaagaa aaattcgttg
      481 gcggcggagt ctttctgttc gccgccgtgt tggagaactt ttttggttta tacacttcgc
      541 taaattcttt tacccagctc gttgctaaat cgcgacaacg cgaggagccc cttaagcgat
      601 ggccacccag agccggagag cagatccttc tctagagcac atcctgttcg aagaagggta
      661 tcggttcgac ttttttcaag cggtgcgcgt cctggaacgc ctttcttcgg cgcgactgcc
      721 cgtcggacgc agttccattc cttcgaagga ggcggtgcgc ttccggtcgc ttcttgctct
      781 cagttttccg gccagctcga tttatgatgt cgctcagtcc gacaatggtg acagtccacc
      841 ggagatggtc gtggcgttta tgggcttgtt cggtcttgcc ggggtactgc cgcggcatta
      901 cacagagctt ctgctcgagc gggtgcgtca gaaagatttc gctctgcgcg atttcctcga
      961 tctctttaac caccggatga tctccctttt ctatcgtgcc tgggagaaat atcgttttcc
     1021 ggtagcgtat gagcgttccc gctcttcacc tggcgctact catgatccat tctcgtttgc
     1081 gctgtttaat ctgattggaa tgggaacgga tggtctcaga ggcaagctaa aatccggcga
     1141 tgaacctctg ctgtattaca gtggcttaat cgctcaacag ccgcgttcgg ctagcgcgct
     1201 ccaagctatg ctgggtgatt atttcaacgt gccggtgatc gtgcggcagt tcatcggcgc
     1261 ttggcttcat gtggataacg aaaaccgcac ccggctcggc cgtggtgatt cgaacaacga
     1321 gcttggaagc acaacgatgt tagggtcaag gtgctgggat caacaagcgg gttttacttt
     1381 agccgtgggc ccgctcaaat ttaatcagtt tcgccaattt cttccttcgg agactggatt
     1441 tagttcgctc gttgaactga cccgtttttt tgccggttta acacttgatt ttaatgtcca
     1501 attggtttta caagcagcag aagtgccggg ttgtcgattg accaggccag cgccggaggc
     1561 gcttcgactc ggttggtcaa gctggctaaa gacaaaagag ttttctcgcg atgcgcaggc
     1621 ggttctagct agccatttaa cgcgcatgca aaaattatct ggccaccctt cgcaggcaac
     1681 attaaaggag gcaggcctcc atgaacgtca atcttaagtc cctcatcggc aaattaaaca
     1741 acacgggccg ccaggcgctc gaaggcgccg caggtttgtg cgtcactcgc actcattatg
     1801 atatcgatat cgagcatgtg atgcttaagc ttgtcgaagc tgagaatacc gatattcaga
     1861 aaattctccg acactatgaa gtcgatcaaa ccagactctc gcgcgatctg acgcgcgggc
     1921 tcgatcggtt gaagaccggc aacgcgcgca caccggcgtt ttccccgaga attccccggc
     1981 tcatcaccga agcctggaat ctagcttcca tcgactttgg cgcgactaag gttcgctccg
     2041 gccatctgct actggcgatc ctgggcgagg aagatctccg gcgtctggcg cgggaggtgt
     2101 cacccgagct gcaaaaggtc tcagttgagg agctgcacaa gcatttcgcc gatctgacag
     2161 ccggaagcgc tgaggatgaa atggccagtc cggcaagcgc cgagaccgcc gatccggaca
     2221 tgcgcggacc gacgaaaacg cctgcgcttg atcagttcac catcgatttg accgatcgcg
     2281 cgcgtaaagg tcaaatcgat ccggtgctgg ggcgtgactt cgagatccgg cagatggtcg
     2341 atattctcac ccgtcggcga cagaacaatc cgatcctgac aggcgaagcg ggggtgggca
     2401 aaacagccgt tgttgaggga ttggcattgc gcattgcgca aggcgatgtg cctgcgccgc
     2461 ttaaaaacgt cgcattgaag tcgctcgatc taactttgct gcaggcggga gccggcgtta
     2521 aaggtgaatt cgaaaaccgg ttgaagtccg tcatcgaaga agtcaaatca tcggcgcaac
     2581 cgattattct ttttatcgac gaagcgcata cgatgatcgg cgccggcggg caggcgggcc
     2641 aaggcgatgc cgccaatatt ttgaagccgg cgctggcgag aggggagttg cgtaccatcg
     2701 cggcgacgac ttggtctgaa tacaaaaaat atttcgagaa agacgctgct ctaaccaggc
     2761 gctttcaggt ggtcaaagtc gacgagccga ccgaagagca ggcagtggcg atgctgcgcg
     2821 ggctgatgag taccctcgaa agtcatcaca acgttcggat tctcgatgag gcgatggatg
     2881 cagcggtgcg gctttcgcac cgttacatca ccggcaggca gctgcctgac aaggcggtga
     2941 gcgtgctcga taccagttcg gcacgagtgg cgttgggcca gatgacgaca ccgccggcgc
     3001 ttgaagactg tcagcggcgt atcgctcagc ttgacactga gatgggaatt ctcgagcggg
     3061 aggctctcac cggcggaaac acgaatgagc gtatcgagag cctggccaaa gagaaggctg
     3121 cgatcgaagg tcgtctagct gagctcaaca accgttggag tgaggaaaaa caacgggtcg
     3181 aagagttgcg cgggctcagg gagaaactcg aggcgcatgc ggccgcaagc aaacaagcgg
     3241 gcaatggcga aacagacgga aagctgtctc ctgaggaagt gaaacaggtg cagggtgaga
     3301 tcgcgcgcct caatgatgaa ctcgccaaaa ttcaaggcga gagcccgctg atgcaagttt
     3361 gcgtagatgc tcaggccgtt gctgaagtag tttcgggctg gacgggaatt cccgtcggca
     3421 aagttctgaa agacgagatc cagacggtgc tttcgctcaa agatcggttg gaggagcgca
     3481 tcatcggcca gtcccatgcg ctcgaagcga tcgcccagag gattcgcacg gcgcgcgcga
     3541 agctggaaga tccacgccgg ccgatcggcg tattcatgct cgtcggcccc agcggcgtgg
     3601 gcaagaccga aactgccctc gcattggcgg acacattgtt tggcggcgag cgcaacgtgg
     3661 tggtcatcaa tatgtcggaa tatcaagaag gccacacggt atcgggtctc aagggctcgc
     3721 cgccgggata tgtcggttac ggcgaaggcg gcgtgctcac ggaagccgtc aggcgcaagc
     3781 cttactcggt cgtactgctc gacgaaatgg aaaaagcgca caccgatgtc atggagcttt
     3841 tctatcaggt ttttgacaaa ggcatgttgg aggacggtga aggcagggaa atcgatttca
     3901 agaacacact aattctcatg acctccaatg ccggtaccga tacgatcgcc aagctctgcg
     3961 ccgaccctga caccaagccg gagccggacg cgctcgccga cgcgctcagg cctgacctgc
     4021 tcaaggtttt caagccggcg tttctgggcc gcgcgatcat cgttccttat tatccgatcg
     4081 ccgataacat catgaagaag atcatcgagc ttcagctcgg gagaattcaa aagcggctgt
     4141 ttgaaaatca ccgcgcgcaa ttctcctacg atccggcttt ggtcgatgag gtggcgcgcc
     4201 gttgcaccga agtcgagagc ggcgcgcgca atgtggatca tattctgacg cgcaccttgt
     4261 tgccggaaat atcgaaagag tttctggcga agatggccgc tggtgaggga atcagtaggg
     4321 tgcacgtatc agttggcggt cagggcggtt ttcagtacaa gattgcatga aggctttcat
     4381 gcggcggcac gattcgccgt tttagggtta tgtcctcgag cttgagggcg cagtcatgaa
     4441 ttacactcaa caagatcggg cgatggcggt tactactccg ctgggcgcgg ataagctgtt
     4501 gctgctgtcg ttcaccggtc aagaggcgat ctctcggctg ttcgattttc aactggatct
     4561 cacggccgaa aatgaggtcg acgtcgcttt tgacaaactc ctaggccaga agattaccac
     4621 caggcttgat ttaccggacg gcgagaagcg ctacttcagc ggcatctgta atcgtgtggg
     4681 ccagggcgaa cgagacgaga tttttactcg atatc
//
LOCUS       DASUTG010000051         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_451972, whole genome shotgun sequence.
ACCESSION   DASUTG010000051 DASUTG010000000
VERSION     DASUTG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451972"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1282)
                     /locus_tag="VF089_01095"
     CDS             complement(50..1282)
                     /locus_tag="VF089_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6172579.1"
                     /translation="MSDRVESTHSEVVRDVSHGSFLEVLWVSIRLGLTSFGGPIAHLG
                     YFHEEYVKRREWIDEQSYADLVALCQFLPGPASSQVSIAIGILHARFLGGIAAWIGFT
                     LPSALALIAFGLGIGAFAGASDAGWLHGLKVVAVAVVAQAVWGMARSLCPDRERATIA
                     IVASIFTLAWPSAIGQLSSIVIAGLIGAVIFPGSTSSSHSHTRFPISKKTAIATWIIF
                     FVLLVGLPFYRQISPSHALEVFDSFFRVGSLVFGGGHVVLPLLQTEVVGPGWVTNEQF
                     VAGYGATQAVPGPLFTFSAYLGAVMSPEPNGWTGAFLALVAIFFPSFLLVTGALPFWD
                     LLRSVPIFQSALKGINAAVVGLLLAALYNPVWTSAIYSPADFGLGLVAFGLLMFWKWP
                     PWVVVVLTAVGAEALARI"
     gene            complement(1275..1652)
                     /locus_tag="VF089_01100"
     CDS             complement(1275..1652)
                     /locus_tag="VF089_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172580.1"
                     /translation="MAQRGQAQADQLKALMEEFKRLQKKLRVIHKQTGYEDLHHGVLA
                     LQIAEHTVEETLEHTGLGGEIQRKPNLAAHRKAKEWRNLVKNLRAEAGKFLKTHPSED
                     LETALKALTIAEGSLEEVAEHYE"
     gene            complement(1728..2033)
                     /locus_tag="VF089_01105"
     CDS             complement(1728..2033)
                     /locus_tag="VF089_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6172581.1"
                     /translation="MTDSDLYKGLIRIHILHHASRNPVFGLWFIEELAHHGYKLSPGT
                     LYPILHGMQENGYLRSSVQRAGRQSRRVYRATAKGKKALAAAKEKVSELFGELFEKE"
     gene            2774..3382
                     /locus_tag="VF089_01110"
     CDS             2774..3382
                     /locus_tag="VF089_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6172582.1"
                     /translation="MMTEQLAVDPNLLREEVKSKYRDVAVNPSGEYHFHTGRPLAKRL
                     GYDSRLVDSMPDSAVESFAGVANPFSLRALDKGDRVLDAGSGGGFDCFVAAHQVGPEG
                     RVIGIDMLPDMLEKARRTAESMGHKNVEFREGLLEEIPVEDGWADVVISNGVINLCAD
                     KKRVFSDLWRVLRPGGRLQFGDIANGKPVPESAIRNIDLWTA"
BASE COUNT          895 a         1007 c         1009 g          852 t
ORIGIN      
        1 gcagaagtgt cctttcagga tccgggaccg cgagtcttag attccgatct taaatgcgtg
       61 ctagagcttc ggcgccaacc gcggtcaata caaccaccac ccagggtggc catttccaaa
      121 acatcaaaag gccaaatgca accaggccaa gtccgaaatc agcgggtgaa tagatcgcgc
      181 ttgtccagac cggattgtac agcgccgcga gcaacagtcc gacaacggcc gcattgatgc
      241 ctttcaaagc agactggaat atcggtaccg agcgaagcaa atcccaaaaa ggaagcgcac
      301 ccgtgaccaa aagaaatgat gggaaaaaga tcgcgaccag cgctaagaat gctcctgtcc
      361 acccatttgg ttccggactc ataaccgcgc cgaggtatgc ggaaaatgta aaaagcggtc
      421 ccggcaccgc ctgcgtcgct ccgtaaccgg cgacgaactg ttcgttcgta acccaaccgg
      481 gaccgacaac ttcggtttga agcagcggca ggacaacatg gccgccgccg aaaaccaacg
      541 aaccgacacg gaagaaactg tcaaaaacct caagcgcatg gctcggcgat atttgccgat
      601 agaacggcag cccaacgagc agcacgaaaa atatgatcca agtggctatt gcagtttttt
      661 tgctgatggg aaagcgcgtg tgagagtgtg aagaagaagt ggacccgggg aagatcacag
      721 cgccaattaa tccggcgatg acgattgatg aaagctggcc gatcgcactt ggccacgcca
      781 atgtgaatat ggaagcgaca atggcaatcg tcgctcgttc acggtccgga cagagagaac
      841 gcgccatccc ccagacggct tgtgcaacga cggcaacggc gacgactttc aggccatgca
      901 accatcccgc atcgctggct ccggcgaacg caccgatccc caaaccgaac gcgatcagcg
      961 caagggcgga gggcaaggta aacccgatcc aagctgcgat cccgccaagg aaacgcgcgt
     1021 ggaggatgcc tatcgcaatg ctgacctggc tacttgccgg ccccggcaga aattggcaaa
     1081 gcgccaccag atcggcataa ctttgctcat cgatccattc tctccgtttg acgtactcct
     1141 catggaaata accgaggtgc gcgatgggac cgccaaaaga agtcagtccc agccgtatcg
     1201 agacccaaag gacctcgaga aaagaaccgt gacttacatc ccttacaacc tcactgtgcg
     1261 tactttcaac gcggtcactc atagtgctcg gctacctctt cgagcgaacc ctccgcgatg
     1321 gtcaaagctt ttagagcggt ctctaaatct tcactcgggt gagtcttgag aaactttccg
     1381 gcctcggcgc gcaagttctt gactaggtta cgccattctt ttgctttcct gtgtgccgcc
     1441 aggtttggtt tcctctggat ctctccaccc aatccggtgt gttcaagagt ttcttccacc
     1501 gtatgttcag cgatttgtag cgcgagcacc ccgtgatgga ggtcttcgta gccagtttgt
     1561 ttgtggataa ctcgtagctt tttttgaagt cgtttgaatt cctccataag agccttcagc
     1621 tgatccgctt gagcttgtcc tctttgagcc attgtccttt ctccttccgt gctttttagt
     1681 ttaggtggca gctcccatac acatggagac gatctctcct gctcagctca ctccttctca
     1741 aataactcac caaagagctc gctcaccttt tcttttgcag cggcaagagc cttctttcct
     1801 ttcgcggtgg cccgatagac ccggcgcgac tggcgtcctg ctcgctgcac cgaggaacgc
     1861 aaatagccgt tctcttgcat accatgaaga atggggtaca acgtgccggg actgagcttg
     1921 tagccgtgat gcgccagctc ctcaataaac cacaacccga acaccggatt tctagaagcg
     1981 tgatgcagga tgtgaatgcg gatcaatccc ttgtataaat ctgaatcagt cacgacggaa
     2041 acctaacgct tttcgttatc gattatcgat atataacagg atgacgcgat ttacaattcc
     2101 gtacgaacaa gattcgcttc tgatcacttt tttgagctcc ggttgatcta gaacgaaacg
     2161 agatttgcct gttacttcag tagctcagtc cgctgctctt ctttcaatca cgtgtctgtg
     2221 tgaagtcggc ggaggccgct tgatctgaca atcttaggcc aagctcgcaa ccgatctatg
     2281 agtctctgcg gccggctgat gctcctagta cggatcaatc gctggctttt gaccccgtgc
     2341 atttgatcgg gttatgcgat tacggatcat tctttatcgt ttactccgta ctgggggatt
     2401 ggatatcgga ccgatcgcag ttactcgccc tctgcatcgg cgacgcttga agattgaagc
     2461 aaccaaggtc cggctgactc aaggttcgtg ccataaccac gtacgaatgc acgctggcca
     2521 gttcgaaggc gtagtgtctg gagaagtaat tgtgcggcgc tggaatagtt cgttgcaatg
     2581 tagcgcaaaa ctgaagattg gcgaggcaaa cgtgatgcgt gaaggtagca tgttcaaacc
     2641 atccagggtg ttgcttcgat gctaacgtgg atacttgctc ttgacataga ctcgttaagt
     2701 gcgactattt ttgggtcgta cccgccagcc ctgcgcaacg aattggtttt aaggacatag
     2761 gtaaggagga tctatgatga ccgaacagtt ggccgttgat ccgaaccttc tccgagaaga
     2821 agttaaaagc aagtatcgcg acgtcgctgt caatccaagc ggcgagtatc acttccatac
     2881 aggcaggcca ctagcgaaac gcctgggtta tgattcgagg ctcgtggact cgatgcctga
     2941 ttctgccgtg gagtcgtttg ccggggtggc taatccgttc tcacttcgag cgctcgataa
     3001 aggggaccga gtgctcgacg caggatcagg cggcgggttc gactgctttg tcgcggctca
     3061 tcaggttggc ccagagggca gagtgatcgg cattgatatg cttccggata tgctcgagaa
     3121 ggcgcgtagg acggctgaat ccatgggaca caagaacgtc gaatttcgtg aggggctcct
     3181 ggaagaaatc ccggtggagg acggttgggc ggatgtcgtc atcagtaacg gcgtcattaa
     3241 tctctgcgcg gacaaaaagc gagtcttctc cgatctatgg cgggtgttgc gtcccggtgg
     3301 aagattgcag ttcggcgata tcgctaacgg caagccggtc cccgagagtg cgatacgtaa
     3361 tatcgatctc tggacggctt gaatcgccgg cggccttccg tgcgaaggct ggcggcggat
     3421 gcttgcggat accggcttcg tggacattca gatcagtcaa ccgtatgaca ctttcgggga
     3481 agcgagaggt gaaaagaagg cgagactctt cgatgtctac ggatacacgt tcctggcacg
     3541 taaacctgga taaggaggtc ccagaccggc acgggtttgc caaaggtttg tctcttaata
     3601 aagaggtaga cgcactctgc ttcggcactt gtttaaatcc taagccgcaa gctccatcaa
     3661 ctccgattga gctcgccgga cattgccggc gagcgggacc gacaggtcta tccaacacgc
     3721 cttatacttt gcgtgaagcg caccgacacg catcgtgctc ttg
//
LOCUS       DASUTG010000052         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1934804, whole genome shotgun sequence.
ACCESSION   DASUTG010000052 DASUTG010000000
VERSION     DASUTG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934804"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1088
                     /gene="pdxA"
                     /locus_tag="VF089_01115"
     CDS             63..1088
                     /gene="pdxA"
                     /locus_tag="VF089_01115"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX6172583.1"
                     /translation="MSRKPIVAITMGDPAGVGPEVILKALSHHAVGRACNPLILGDWG
                     VLQRVRARIKRLPKLISWQSGVPLLPLLRGTGGFVVCPLSTLRENESRPGRPVKAGGH
                     AAYRYITVAARLALSNVADAIATAPISKSILIDAGYNYPGHTELLAELSRTPECRMML
                     IGKKLRVVLVTGHVGYAKVVRSLNSDGIRITLELTYRALRNSFGISRPRIAVAALNPH
                     GGEEGIFGSEEKEIIVPGIQAARRKGVPAAGPFPADSLFHKAARGEHDAVVCMYHDQG
                     LIPLKLHHFFGGVALTLGPPFIRTSVDHGTAYDIAGTGRADETSMVQSILLAAELARQ
                     KKKRNNH"
     gene            1085..2437
                     /locus_tag="VF089_01120"
     CDS             1085..2437
                     /locus_tag="VF089_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6172584.1"
                     /translation="MNPAIFREYDIRGVAEKEFDEDFALLLGKVHGTIINEAGGARVS
                     VGRDCRATSERYTQAVIAGLVSTGLQVVDIGVCPTPLLYFSLFHLDTDGGIEITASHN
                     PSEYNGFKLCLGKDTLYGAQIQDIKARMEQNHFVENPGGGVKSYNIVPPYQQHLLHDV
                     PKLKRSLNVVVDAGSGVGGPVAPPIFRRLGCTVWEIACNPDGNFPIHHPDPTVPENLE
                     LLIHKVRTEGADLGIAYDGDADRIGAVDEQGNIVWGDELLMIFARDVLQRNPKATIIS
                     EVKCSQRLYDDIVARGGIPIMWKAGHSLLKAKLKETNALLAGEMSGHMFFKDRYFGYD
                     DAIYASLRLLEIIANSDGPFSTLLADLPRTVSTPEIRIDCPDDRKFIIAERAKEYFRQ
                     HYDVIDIDGVRVKFPEGWGLIRASNTQPALVLRFEAQTAEKMTEYRALVEGKLKELEI
                     "
     gene            2447..>3837
                     /gene="uvrA"
                     /locus_tag="VF089_01125"
     CDS             2447..>3837
                     /gene="uvrA"
                     /locus_tag="VF089_01125"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HEX6172585.1"
                     /translation="MRGEIVVSGARVHNLKNIDLKIPREQLVVITGVSGSGKSSLAFD
                     TLYAEGQRRYIESLPATSRQLLQQLDKPDVDSINGLSPAIALQQRPSIFGPRSTVGTM
                     SEVQDFLRLLFTRIGQPFCIHCGARISIQTTEQIVDQLMALHPGARIVLMAPIAGARK
                     LNSNELLREFTRQGFTRVRIDGRLHELDQDTHLESGRTYRIDLVIDRLTIREGVEKRL
                     ADSLETASRVGQQVVSVEVQAGTTELKELRFSQKFACAECGSSFPEIVPSLFSFNSPN
                     GACPVCAGLGSSEQAPGKSKVDFDSSHIEPCKECSGARLRKESLAVRIDGKNIAQVAA
                     LPINEALQYFDALQFGPPEQITAGKILAEIIPRLGFLARLGLDYLTLDRSSTTLSGGE
                     LQRVRLAKQLGSKLSGVLYIFDEPSIGLHQKDNAQLLELLQGLRSAGNSILIVEHDPE
                     TILSADYVIDMGPG"
BASE COUNT          866 a         1020 c         1069 g          882 t
ORIGIN      
        1 ccttcaaacg ctaaaccgct ggttcttcgt taagcagcct agtctcggat ccgtttaagc
       61 ttatgtcgcg taaaccgata gtagccataa ctatgggcga tcccgcaggc gtaggtcctg
      121 aagtcatttt gaaggcgctc agccatcacg cggttgggcg ggcatgcaat ccgctgattc
      181 ttggcgactg gggagttctc caacgggtac gtgcaagaat taaacggctg cctaagctaa
      241 tctcctggca gtcgggagtg ccgttgctgc ctttgttgcg aggcactggg ggcttcgtcg
      301 tctgcccgtt gtctaccttg cgggaaaacg aatcgcgccc gggccggccg gtgaaagccg
      361 gcggccatgc ggcgtatcgc tatataaccg tggcggcccg gctcgccctc tcgaatgtag
      421 ccgatgcgat tgcgacggcg cccatcagca agagtatttt gatcgatgcc ggttataact
      481 atcccggaca tactgaactt cttgccgaac tcagccggac gcccgaatgc cgcatgatgc
      541 tcatcggcaa aaaactgcga gttgtcctgg tgaccgggca tgtcggttat gccaaggtgg
      601 tgcgctcgct gaactctgac ggcattcgta tcaccctgga gctgacctat cgagctttga
      661 gaaattcctt cgggatctcg cggccgcgca tcgctgtagc ggctttgaat ccgcacggcg
      721 gtgaagaagg gatcttcgga agcgaagaaa aggagatcat cgtgcccgga attcaggctg
      781 caagacgcaa gggagtcccg gcagccgggc cgtttccagc cgatagtctg tttcacaaag
      841 ccgctcgcgg agaacatgac gcggtagtct gcatgtatca cgatcaggga ttgatccccc
      901 tcaagctgca tcattttttt ggcggagtgg cgctcacctt agggcctccc ttcattcgaa
      961 cttcggtcga ccacggcacc gcgtatgaca tcgctggcac tggcagagcc gacgagacca
     1021 gtatggtgca atcgattttg ctcgcggccg aattagctag gcaaaaaaag aagcgtaaca
     1081 atcattgaat cccgcaattt ttcgcgaata cgacatccgg ggggtggcag agaaagagtt
     1141 cgatgaagat tttgcgcttt tgcttggcaa agtgcacggc acaatcatca atgaagcggg
     1201 cggtgcccgc gttagtgtcg gccgggactg ccgggcgact tccgagcgtt atacccaggc
     1261 tgtcatagcc gggctagttt cgacagggct gcaagtcgtc gacattggcg tctgtccgac
     1321 accattgctt tatttctcgc tgttccatct cgacaccgac ggcggaatcg agatcaccgc
     1381 cagccataat ccctcagagt acaatggatt caagctttgc cttggcaagg acacgcttta
     1441 cggtgcgcaa atccaggaca taaaagctag aatggagcaa aatcattttg tcgagaatcc
     1501 cgggggtgga gtgaagtcct ataatatcgt gccgccttac caacaacatc ttttgcacga
     1561 tgttcctaag ttaaagcggt ctctcaatgt agtcgtcgat gccggtagcg gcgtgggtgg
     1621 accggtggcg ccgccgattt tccgtcgtct gggctgcacg gtttgggaaa tcgcctgtaa
     1681 tcctgacggt aattttccga tccatcatcc cgatcctact gtccctgaaa atcttgagct
     1741 tctcatccac aaagttcgca cagaaggggc ggatctagga atagcttatg acggagatgc
     1801 ggatcgcatc ggcgccgtgg acgaacaggg aaatatcgtt tggggtgatg agctgttaat
     1861 gatttttgcc cgagatgttc tccagcgtaa ccccaaagcg acgatcattt ctgaggttaa
     1921 atgctcgcaa cggctttacg acgatatcgt cgcgcgcggc ggcataccga tcatgtggaa
     1981 agcgggccac tccctgttaa aggctaagtt gaaggaaacc aacgctttgc tggcgggcga
     2041 gatgagcggc catatgttct ttaaggatcg ctactttggt tacgacgatg cgatctatgc
     2101 ttcgctgcga ttgcttgaga ttatcgccaa ttctgatgga cctttctcaa ccttgcttgc
     2161 ggacctgccc cggactgtat cgacacccga aatcagaatc gactgcccgg acgataggaa
     2221 atttatcatc gccgaacggg caaaggagta ttttcgccag cactacgatg tcatcgacat
     2281 tgacggcgtg cgcgtaaagt tccccgaagg atggggactg atccgcgcat cgaatacgca
     2341 gcctgcactg gtgcttcgtt ttgaggctca aaccgcggag aagatgacag aatatcgcgc
     2401 cttggtcgaa ggcaagctta aggaactgga gatctgacag ccaataatgc gtggcgagat
     2461 cgttgtcagc ggggcgcgag tccacaacct taaaaatatc gacctgaaga ttccgcgcga
     2521 gcagttagtg gtcatcaccg gagtttccgg ttcaggaaaa tcgtccctag ccttcgatac
     2581 actttacgcg gaaggccagc ggcggtatat cgaatcgcta cccgccacta gtcgtcagtt
     2641 acttcagcag cttgacaagc cagacgtcga ctctatcaac ggactttctc ccgccatcgc
     2701 acttcaacaa agaccgtcca tctttggccc gcgctcgacg gtcggaacga tgagcgaggt
     2761 gcaagatttt ttgcggctgt tgttcactcg tatcggccag ccgttttgca ttcactgcgg
     2821 ggcgcgaatc tcgatccaga ctacagaaca aatagtcgat caacttatgg ctcttcaccc
     2881 gggcgcgcgc atcgttctta tggcgccgat tgccggcgca agaaaactga attcgaatga
     2941 attgctccgc gaattcactc gccagggctt cacccgtgtc aggattgacg gccgactaca
     3001 cgagcttgat caggatactc acctggaatc aggccggact taccgaatcg acttagttat
     3061 cgaccgcttg acgattagag aaggcgtgga aaagcgcctg gcggattcgc ttgaaacggc
     3121 ttctcgtgtc ggccagcagg ttgtaagcgt tgaagtgcag gcaggtacca cggagctcaa
     3181 agaattgcgc ttcagtcaaa agtttgcctg tgctgaatgt ggcagttctt ttccggagat
     3241 tgttccgagt ctgttttcct tcaatagccc taacggcgca tgtccggtgt gcgctgggtt
     3301 gggtagttcg gagcaggcgc ctggcaaatc aaaagtcgac tttgacagca gccatattga
     3361 gccctgcaaa gaatgcagcg gcgctcgtct gaggaaagaa agcttggcgg tccggatcga
     3421 cggcaaaaat atcgcgcaag tggcggcatt gcccatcaac gaggcacttc aatatttcga
     3481 cgcgctccag tttggacccc cagagcagat caccgccggc aaaatacttg ccgagatcat
     3541 cccccggctt ggtttcctgg cgcgactcgg actcgattat ctgactctcg atcggtcatc
     3601 gaccaccttg tcggggggcg aattgcaaag ggtccgatta gccaagcaac tgggctcgaa
     3661 gctgtcgggc gtactatata tctttgatga gcccagcatt ggcctccatc aaaaagacaa
     3721 tgcgcaactg ctggagcttc tacaggggct tcgtagcgcg ggtaattcca tcttgatcgt
     3781 cgagcatgat ccggaaacta ttctcagcgc cgattacgtc atcgatatgg gcccggg
//
LOCUS       DASUTG010000053         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_452013, whole genome shotgun sequence.
ACCESSION   DASUTG010000053 DASUTG010000000
VERSION     DASUTG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452013"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF089_01130"
     CDS             complement(<1..304)
                     /locus_tag="VF089_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX6172586.1"
                     /translation="MMEFLNPAALYGFFALPLLLIPYLIRRKPRRVVFSSLLLFLGQA
                     TQATAKPWGRLRLPPVFFLQLLLLMLLILALAEPVFSVRVSNIAIVMDNSASMQAQE"
     gene            complement(311..1204)
                     /locus_tag="VF089_01135"
     CDS             complement(311..1204)
                     /locus_tag="VF089_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6172587.1"
                     /translation="MLPEPFTPQFLTQLETLRLRTRKEFLGSHTGSYSSPRRGTSLEF
                     ADYRRYSPGDDLRYLDWGIFARTDRPYIKVFREEVDLFAYVFIDASASMGFPSSQEKF
                     LPAAHIGLALSYVILANHDHVKLHLLHGESISPASPFYRGRRRMADCVRFATSLEPRG
                     LLDLARSLGEHLKKLRRPGKAILISDFLMPPQSYQRGLNLLRALNLDIAAIQVLSRPE
                     IDPPFTNGGVNLVDSETQSEIRLQWSPRARQEYQAKLAHHNSELRSFCHQSGIHYSLY
                     VNDRDLGEFVFATLPAIGLFK"
     gene            complement(1213..1914)
                     /gene="queC"
                     /locus_tag="VF089_01140"
     CDS             complement(1213..1914)
                     /gene="queC"
                     /locus_tag="VF089_01140"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX6172588.1"
                     /translation="MARRKAVILLSGGIDSATAAAIAQRDHFDLHALTFRYGQRHERE
                     IESAKKVANWLRVKEHLVIESGLRAIGGSALTDRIEVPKGRSTSEIGDGVPVTYVPAR
                     NTIFLAYGLALAEKSGASDIFFGANQLDYSGYPDCREEYVHAFENMANLATKAGVEGK
                     VQLKIHTPLIQMTKAEIIKQGSELGLDYSLTWSCYDPTVDGKACALCDSCQLRLNGFK
                     EAGLSDPIPYAAVAG"
     gene            complement(2063..2389)
                     /locus_tag="VF089_01145"
     CDS             complement(2063..2389)
                     /locus_tag="VF089_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6172589.1"
                     /translation="MKVSDLRKILFLGVTVVSLVAYYARASADPSKYPQFAQQSLPAN
                     VPLALTSIDELVVDVKAGKKPLIIDVRTSAEYNEVHILGAVSAPLAQFKEYMKSIPRD
                     RPVVLY"
     gene            complement(2386..3267)
                     /locus_tag="VF089_01150"
     CDS             complement(2386..3267)
                     /locus_tag="VF089_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX6172590.1"
                     /translation="MAKTSKNRPTSNIQTAAPSTAKQQPNWPILALAISGMLLSGYLT
                     YSAWQGQSVAGCTVGSACDIVLNSRWSKLLGIPISFWGFLTYALLAAIAWNKTTPSQW
                     KMAWTVTIIGVLYSLYLTGVSLIVLDSACPYCLTSLGLMTAIFAVLLTRRPAKMPGFS
                     WGPWLGKTAGAAVLIIIVLHLHYSGYIGKTAGPEDPWIRGLVEHLAKTDAKFYGASWC
                     PHCTEQKELFGPSSSRVPYIECSPGGPRAPQAQACNDAGIKSYPTWIINGQRYSGIQS
                     LDSLAQYSDYKSAGAKK"
     gene            complement(3280..>3530)
                     /locus_tag="VF089_01155"
     CDS             complement(3280..>3530)
                     /locus_tag="VF089_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6172591.1"
                     /translation="DRFKASTDRWLFLTGGQEEIKNLVQNGFRLSVATASSDPGVILH
                     SPRLVLIDRQAQIRGYYDSRDNEALQRLRRDVAILVKG"
BASE COUNT          833 a          919 c          970 g          808 t
ORIGIN      
        1 cctcctgggc ctgcatgcta gccgagttgt ccattacgat ggcgatgttg gatactcgga
       61 cggagaatac cggttcggca agcgccagaa tcagtaacat caataggagc aactgaagaa
      121 agaaaacggg cggaagacgc aaccggcccc acggtttagc tgttgcttgc gtagcttgcc
      181 ccaaaaacaa tagcaggctt gagaagacca cgcggcgtgg tttgcgccgg atgagataag
      241 gaattaggag caagggtaac gcaaagaatc cgtagagagc tgcgggattg agaaactcca
      301 tcatgcaagc ttatttgaac agaccaatcg cgggtaaggt cgcgaaaacg aactccccca
      361 aatcgcgatc attaacatag agggagtagt gaatgccgct ttgatggcaa aagctgcgca
      421 gttctgagtt gtgatgcgcc agcttggctt gatactcttg ccgcgctcgg ggactccact
      481 gcagccggat ttcactttgg gtttcactgt ccacaaggtt tacgccacca ttggtaaacg
      541 gcgggtctat ttctggccgg ctcaaaacct ggatcgccgc gatgtcgaga ttgagcgctc
      601 gtagcaagtt cagcccgcgc tggtaggact gcggcggcat caagaaatcc gagatcaaga
      661 ttgccttgcc tgggcgccga agtttcttaa ggtgttctcc tagcgatctc gctaaatcga
      721 ggagaccacg cggctccaat gatgtagcga atcgcacgca atccgccatg cggcggcggc
      781 cgcgataaaa cggcgatgcc gggctgatgg attcgccatg caataaatgg agcttgacat
      841 gatcatggtt tgccaaaatt acataggaga gcgccaatcc gatgtgcgca gctggtaaga
      901 acttctcttg gctcgacgga aatcccattg atgcgctggc atcgatgaaa acgtaggcga
      961 agagatcaac ctcttcccgg aagaccttga tataaggccg atcggtgcgc gcgaagatcc
     1021 cccaatccaa atagcgcaga tcatcccctg gggaataacg ccgatagtcg gcaaactcca
     1081 agcttgtgcc gcgccggggc gatgaataac tgccggtgtg gctgccgaga aactctttcc
     1141 tggtgcgtaa acgaagagtt tccaattgtg ttaggaattg cggggtaaac ggttcgggga
     1201 gcattgaaaa atctagcctg cgacagctgc gtaagggatc ggatcgctaa ggcctgcctc
     1261 tttgaagccg tttagcctga gctggcagct atcgcacaga gcgcaagctt tgccgtccac
     1321 agttggatcg tagcaactcc aggtaaggct gtagtcgagt cccagttcgc tcccttgctt
     1381 aatgatttct gctttcgtca tttggattaa cggggtgtga atcttcaact gcactttgcc
     1441 ttcgaccccc gctttggtcg ccagattggc catgttctca aaggcgtgaa cgtattcctc
     1501 gcggcaatcg ggatagccgc tgtagtcaag ctgattggcg ccaaaaaaaa tgtccgacgc
     1561 tccactcttt tctgccaatg ccaaaccgta tgctagaaag atggtattgc gcgccggcac
     1621 gtaagttacc ggtacgccgt ccccgatttc cgatgtggag cggcctttgg gaacttctat
     1681 acggtcagtc agagccgaac cgccaattgc acgtaggccg gattctatga ctaggtgctc
     1741 cttgactcgt aaccaattcg cgaccttttt agccgattcg atctcacgtt cgtggcgttg
     1801 gccgtagcgg aaggtgaggg cgtgaagatc gaaatgatcg cgctgcgcaa tcgctgctgc
     1861 agtggcggag tcgatgccac cgctaagtaa gatgacagct tttcttcgcg ccatgattca
     1921 tgattcaagg aaccttggtt ccttccatcg gataacgtct ttcgtgccag ccgatcaagc
     1981 cttcgttaag aaccttcata ttgcgatagc cattttcata gagaatgcca taggccagac
     2041 tggccagatg atgtgggcag gctcaataga gaacgaccgg ccggtctctc ggaatactct
     2101 tcatgtattc tttgaattga gccagcgggg ctgagacggc gccgaggata tgaacttcgt
     2161 tgtattccgc gctggttcgc acatcgataa tcaacggctt tttgccggct ttcacgtcaa
     2221 cgacgagctc atcgatggac gtaagtgcca gcggcacgtt ggcaggcagt gattgttggg
     2281 cgaactgagg gtacttagag ggatcggcgc tcgccctcgc ataatacgcc accagggata
     2341 ccactgtcac ccctaggaaa agaatcttcc gtaaatcaga taccttcatt tctttgctcc
     2401 tgccgacttg taatcgctgt actgagccaa gctgtccaac gattggatgc cggaatagcg
     2461 ttgcccgttg ataatccaag tcggataact cttgatcccc gcgtcattgc atgcttgcgc
     2521 ttgcggcgcg cgcggcccgc ctgggctgca ttcaatgtac gggactcgcg acgatgacgg
     2581 gccaaaaagc tccttctgct cggtgcagtg gggacaccag gaggcgccat agaatttggc
     2641 atcggtttta gctagatgtt caaccagccc gcgaatccat ggatcctccg ggccggcggt
     2701 ctttccgata tagcccgagt aatgaagatg gaggacgatg atgatcagaa ccgccgctcc
     2761 ggcggttttc ccaagccatg gtccccaaga aaatcccggc attttagcag gccggcgcgt
     2821 gaggagcacg gcaaaaatcg ccgtcatcaa tccaagtgaa gtcaagcaat aaggacaagc
     2881 ggagtccaaa acgatcagtg aaacaccggt aaggtagaga ctataaagta ctccgataat
     2941 ggtgacggtc caagccatct tccactgact aggcgtcgtt ttgttccagg cgatggcggc
     3001 taaaagagcg taggtcaaaa agccccaaaa cgaaatcggt atgcccaaca gtttcgacca
     3061 gcggctattg agtacgatgt cgcaggcgct gccgaccgtg catccggcta ctgactggcc
     3121 ctgccaagcg gaataggtca gataaccgga caggagcata ccggaaatcg ccaaagcgag
     3181 tatcggccag ttcggttgct gtttggctgt gctcggcgca gctgtttgga tgtttgaggt
     3241 tggcctgttc ttacttgtct ttgccatttt ataaactcct caacccttca ctaagatggc
     3301 cacatctctg cggagacgct gtagcgcttc gttatcccgg ctatcgtaat aaccgcgaat
     3361 ttgcgcttgc cggtcgatca gtaccaatct cgggctatgc aaaatcactc caggatcgct
     3421 cgaggcagtg gcgacgctca agcggaaacc gttctggacc aggtttttta tctcttcttg
     3481 cccgccggtt agaaatagcc aacgatctgt gctggcttta aatcgatctg
//
LOCUS       DASUTG010000054         8052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_155967, whole genome shotgun sequence.
ACCESSION   DASUTG010000054 DASUTG010000000
VERSION     DASUTG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155967"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VF089_01160"
     CDS             <1..888
                     /locus_tag="VF089_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6172592.1"
                     /translation="KFIKTMVYQLNGNELVGALVRGDHEINEVKLQATLGCREIALAN
                     EAAVVAATGVTPGYLGPIGLKLRTIADLSVQSMRSAVTGANEANAHYIEVDQERDFTP
                     SAFADIRLALPGDHCPRCDQGRLEAHRGIEVGQVFYLGKKYSELMGATYLDAEGRERP
                     IEMGCYGIGISRLVAATVEQNHDENGIIWPLSIAPFQVLLLPINYNDEATGEAADLLY
                     GELRQRGLEVLLDDRDERPGVKFKDGDLIGIPLRITIGTKGLQKGSLELRWRREGKSI
                     DIPVTGAAQTIHSYLNKSA"
     gene            898..1671
                     /gene="pyrF"
                     /locus_tag="VF089_01165"
     CDS             898..1671
                     /gene="pyrF"
                     /locus_tag="VF089_01165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX6172593.1"
                     /translation="MTSKLELLRARRSTRERLIVALDVNDLNQAKELVQALANEVGMF
                     KIGKQLFTHAGPQAVRLVQELGGEVFLDLKFHDIPNTVAKAAVEATRMGVKMFNVHAS
                     GSLEMMRTTVKEVRRVCRQEKRRRPIMLAVTVLTSLNQDDLKRVGIDGRVAAQVVRLA
                     LLTNEAGMDGVVASPHEVGAIRDCCGRRFIIVTPGIRPADSSRNDQHRIMTPAAAVKA
                     SVDYIVVGRPILEAKDPIAAARSIVAEMKSSSKEPVGVS"
     gene            complement(1721..2521)
                     /gene="thiD"
                     /locus_tag="VF089_01170"
     CDS             complement(1721..2521)
                     /gene="thiD"
                     /locus_tag="VF089_01170"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6172594.1"
                     /translation="MKIQIYKALTIAGSDSGAGAGIQADLKTFAALGVYGTSAITAIT
                     AQNTLGVSGIVELSPKIVAAQIDAVIDDIGAQAVKTGMLASAAIIETVAERLRAHNLT
                     NVVVDPVMVATSGDLLLEKNAIGALRSQLLPLATVVTPNILEANQLAGLTIANARDIR
                     RAAQRIVDLGAQSVVIKGGHRRGPATDLFFDGKRFRQLRTPRIRTIHTHGTGCTLSAA
                     IAAYLARSEKLETAIMHAKTYITAAIRKGFPVGSGHGPVHHFFRYWGK"
     gene            2864..3001
                     /locus_tag="VF089_01175"
     CDS             2864..3001
                     /locus_tag="VF089_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172595.1"
                     /translation="MNEFLLLALVVIYLTISYPPKSKQPNSNGGERTRRFFALIGKKR
                     G"
     gene            3290..5002
                     /locus_tag="VF089_01180"
     CDS             3290..5002
                     /locus_tag="VF089_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX6172596.1"
                     /translation="MSVDRTSLVSFLNYTPVPLAFGTSGLRGLVKDITDLEAYINVKG
                     ALKYLLSIGDMAAGTEVILAGDLRPSTDRIMRACMQAILDCDCSVENAGKIPTPALVS
                     RAMANGAAGVMVSGSHIPFERNGIKINKSIGEILKSDESGILREVERVRDQEYSRTAA
                     TSAFNADGMLKEPANLPPLDRRAEEAYVRRYTESFTNNGLSGLRVLIYQHSAVGRDIL
                     ARILGELGAAVITAGRSDRFIPIDTENITGEQLDDFEKMISDGEADDQGFDAILSTDG
                     DSDRPLMVAVLPGAKVYPGGRRVRFLPGDLLGIITAEYLGANAAAVPVSANDAVERRM
                     HECGILLRKTKIGSPYVVAALDELRFAGKWERVVGWEANGGFLTGSDVVLAGGTLSAL
                     PTRDSTLPILANLFAAAERKASLAELWDLLPARFGCAGLLDDFPVAASQAILAKLIPT
                     SGLIEVEFGAAGDVFDRSVSDNTAAPLAPSAARDWQQAKTNLIRFFTPAQGFDDIARI
                     NVLDGVRIYFRNGDVAHMRPSGNAPQLRLYACSDFQARAEEIRELALREPDGILRQLE
                     RAFT"
     gene            complement(5027..6067)
                     /locus_tag="VF089_01185"
     CDS             complement(5027..6067)
                     /locus_tag="VF089_01185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6172597.1"
                     /translation="MRFGTFSYNQSRPWVSEKQAFEELLEQILLTERLGFDEAWFAEH
                     HHSDYGLIASPNLMIASLAHRTKRLRLGNLVNVLPLYDPMRLAEECGMLDILTGGRLN
                     VGLGRGVPRDDLKHGLDRDTAQARFEEGIEIMMRSWTEETFSYAGRAWNYVDIVCRPR
                     PLQCPHPPIYYGATSPDSPAMVARRGWNLAVSRQPLANCAQAIRSYRDERAKHNLPGN
                     GDAIMVRDIYVAETDEQAWKEAEPELIRFWQLATDNVWRGDSVSADDLPKFTERFPYF
                     PGGLTIKRLDEWGTSLIGSPQTVVAKARAMIATAKPDSLVGMFSFGGLSHDQVTRSIE
                     LFATKVRPALGL"
     gene            6441..7244
                     /locus_tag="VF089_01190"
     CDS             6441..7244
                     /locus_tag="VF089_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172598.1"
                     /translation="MAKLVLAAGVPHPPRLVYEIAQAPGTTRGEAMMKQVREQVEKAE
                     PDLIIEVDSDHFVNFFYNNLPSFCVGMAEEAEGPQESWCPMPRYTVRGNVPLAKALLR
                     YGVHNRFDLAAAHELRLDHSLVVPLHFLNPGMELPVVPIYTNGFASPLPLASRCLALG
                     RMLRRFIESWEGKERIALIASGCFAMDVGGPLRGWTDTDWAETISSLLLKGRYRTLAT
                     RATEDRINAAGNNSGELLNWITVSGAVGRTRPLFLEGDEGNGYAVWKFD"
     gene            7392..7715
                     /locus_tag="VF089_01195"
     CDS             7392..7715
                     /locus_tag="VF089_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172599.1"
                     /translation="MSVYQLNKLLYLTDNDPEFRKRMLADPEAALSEFKLTSEERDAL
                     TSGAVGKLYQMGVHTFLLNHLYRYQLFGVNRDNYLERIREGMPYDPRFELGNMPVQRF
                     ITKQD"
     gene            complement(7795..>8052)
                     /locus_tag="VF089_01200"
     CDS             complement(7795..>8052)
                     /locus_tag="VF089_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172600.1"
                     /translation="GAGFANVITAKTIEEFEKGFSRALNSNDLYFILSKVEAGAGDVP
                     AAALDSQENKYLFVRYIEKSENLKILTSTLSRASDPVPKSK"
BASE COUNT         1764 a         2221 c         2271 g         1796 t
ORIGIN      
        1 aagttcatca agacaatggt ctatcaactc aacggtaatg agctggtcgg ggcgctggtg
       61 cgcggcgatc atgaaatcaa cgaggtcaaa ctacaagcaa cccttggttg ccgtgagatt
      121 gcgcttgcca atgaagcagc cgttgtcgcc gccaccggag tcacccccgg ctatctagga
      181 ccgatcggct tgaaactgcg aaccattgcc gatctttccg ttcagtcgat gcgcagcgcg
      241 gtgaccggag ccaacgaagc gaacgcccat tacatcgaag tcgatcagga gcgtgatttt
      301 accccgagcg cctttgccga catcaggctg gcgcttcccg gagaccactg cccgcgttgc
      361 gatcaaggcc ggctcgaagc ccatcgcggc atcgaagtgg gacaggtgtt ctatctcgga
      421 aagaaatata gcgaattgat gggtgctacc tatttagatg ccgaaggccg cgagcgcccc
      481 atcgagatgg gttgctatgg catcggcatc agccggctgg tcgccgccac ggttgaacag
      541 aatcacgatg aaaacggcat catctggcca ttatcgattg ccccattcca agttctattg
      601 ctgccgatca actataacga cgaggcgaca ggcgaagcgg ccgacctgtt gtatggggaa
      661 ttgcgccagc gcggcctcga agtgcttctc gacgaccgcg acgagcgacc cggagtcaaa
      721 ttcaaagacg gtgatctgat cggaattcct ttgcgcatca ctatcggcac caaaggattg
      781 cagaagggca gtctcgaatt gcgctggcgt cgtgaaggca aatcgatcga cataccagtc
      841 actggggcgg cgcaaacgat tcacagctat ctcaacaagt cagcctagcc aacgataatg
      901 acctcaaagc tggaactgct tcgggcgcgg cggtcaacgc gcgagcgtct gattgtcgct
      961 ctcgatgtca acgatctcaa tcaggccaag gaattggtgc aggctcttgc caacgaagtg
     1021 ggcatgttca aaatcggcaa gcagttgttc actcacgccg ggccgcaagc cgtgcgcctg
     1081 gttcaagagc tcggcggtga ggtttttctc gatctcaagt ttcacgacat ccccaatacg
     1141 gtcgccaaag cggccgtcga ggctactcgc atgggggtca aaatgttcaa cgttcatgct
     1201 tccggcagtc tcgagatgat gcgtacgacg gtcaaagaag tacggcgcgt atgccggcag
     1261 gaaaagcggc gcaggccgat catgctggct gtcaccgtgc tgaccagttt gaatcaagac
     1321 gatctcaaaa gagtaggcat cgacgggcgg gtggctgctc aagtggtgcg attggcgctg
     1381 ctaacaaacg aagccgggat ggatggcgtg gttgcgtcgc cccatgaggt cggagctatt
     1441 cgcgattgtt gcggccggcg ttttatcatt gtcactccag gcatccgtcc ggcagatagt
     1501 agccgcaatg atcagcaccg catcatgaca ccagcggctg ctgtcaaggc cagcgtcgac
     1561 tatattgttg tcggacgacc cattctcgag gccaaagatc caatcgcggc tgcccggtcg
     1621 atcgtcgctg agatgaaatc ctccagcaag gaacctgtcg gcgtcagctg atccgacgat
     1681 ctagtagtgt ccgccgatgg atcatgaatt gcgcgggacg ctatttaccc caataacgga
     1741 agaagtgatg caccggacca tggccgcttc cgaccgggaa acctttgcgg atggccgcgg
     1801 tgatgtaggt tttggcatgc atgatggcgg tttccagctt ctcacttctg gctaaatagg
     1861 ccgcaatagc tgcggatagt gtgcaacctg tgccatgagt atgaatcgtg cggatgcgcg
     1921 gagtgcgaag ctggcgaaac cttttgccat cgaagaatag gtccgttgcc ggtccgcgcc
     1981 ggtgaccacc cttgatcacc acactctggg ctcccagatc aacgattcgt tgcgccgcgc
     2041 gccggatgtc tcgagcattg gcaatcgtca agcccgccag ttggtttgct tccagaatat
     2101 tcggcgtcac taccgtggcc agcggcagca gttgggaacg aagcgcgccg atggcatttt
     2161 tctccagcag caggtctccc gaggtcgcca ccatcaccgg atcgacaact acgttcgtga
     2221 gattatgagc acgaagtctt tccgcgacgg tttcaataat cgccgcgctg gctaacatcc
     2281 cggtctttac cgcctgggcg ccgatgtcat cgatcaccgc atcgatttgc gccgcgacga
     2341 ttttcggact gagctcaaca atgccgctca ctccgagggt attttgcgct gtgatcgcag
     2401 taatcgccga ggttccataa actcccaaag cggcaaacgt tttcaagtca gcttggatgc
     2461 cggctcccgc tcccgagtcg gagccggcga tggttagagc tttgtagatt tggattttca
     2521 ttttgaccag catcgtagca aagcgcgcag gaagttacga cgcataagca gagaaccgcg
     2581 gcgtcctgcc gaattcacga ttcgagttta tctggacgac cctgccgagg catgaaccgg
     2641 cggaattcga atttccgtga acctttttgt gcagtagtcg taaacttttt ctacgaacgg
     2701 caccttttgt tccgttctgg tcccgagcgc aaaatataaa tactaaattt tatgcgatta
     2761 attcacactc tttgtctcct gcagccgctg gcacatgcgt tgctttggca agcgatcgag
     2821 agagttgtcg aatccaactg cacgaacgaa aaaggttgtg gccatgaacg aatttttgct
     2881 cctagcgctg gtagtcatct atctcacaat ctcgtatccg cccaaatcca aacagccaaa
     2941 cagcaacggc ggcgaacgga cgcgacggtt cttcgcgttg attggaaaga aacgcggtta
     3001 accattcccg cttgagccag gcagatcgtg aactcatcca gttcgagaca aaaagctcca
     3061 gcttcgctcc actctgaatc ctgttcttaa tccatctttc gttccgctct agatataggt
     3121 ttccctgatc tcggccgata gtgaccggcc catccggtgt ctattggatc ttccacccta
     3181 ttagattcac cttatcgaaa aaaaggtcta tataataaag cctaaattca attcattcct
     3241 gtcctcaacg ggtgcgtccg ggccggcgat gtaagcgatc atttatccga tgtccgtaga
     3301 tcgaacgtct ctagttagct ttctcaatta tacgccggtg cccctggctt ttggcaccag
     3361 tggattgcgc ggtctggtca aagacatcac cgatctcgag gcttacatca atgtcaaagg
     3421 cgcgctgaaa tatctgctga gcattggcga catggcggca ggtaccgaag tcattcttgc
     3481 cggtgatttg cggccgagca ccgaccgcat tatgagggcc tgtatgcagg cgatcctcga
     3541 ctgcgattgt tccgtggaga atgccggcaa gattccgacc ccggctttgg tttcacgcgc
     3601 gatggcaaac ggcgctgccg gcgtgatggt tagcggcagc catattcctt tcgagcggaa
     3661 cggcatcaaa atcaataaaa gcattggtga aatactaaag tcagacgagt ccggtattct
     3721 gcgcgaggtg gagcgcgtcc gcgatcaaga gtatagccgg acggcggcca catcagcatt
     3781 caatgcggac gggatgctca aggaacccgc gaatctacca ccgctcgatc gccgcgccga
     3841 agaggcctac gtccgccgtt acaccgagag ttttaccaac aatggcctct cagggttgcg
     3901 cgttctgatc tatcagcatt cggccgtcgg ccgtgacatt ttggctcgca tcctgggtga
     3961 gctgggcgcc gccgtaatca ccgccggacg cagcgatcgg ttcataccca tagatactga
     4021 aaacatcacc ggcgaacagc tggatgattt cgaaaaaatg atttcggacg gcgaagccga
     4081 tgaccagggg tttgatgcta ttttatccac cgacggtgat tcggaccggc cattgatggt
     4141 tgcggttctg cctggggcca aagtttatcc gggcggcagg cgggtgcggt ttttacccgg
     4201 cgatcttcta ggcattatca ctgccgagta cctcggcgcg aacgcggccg cggtgccggt
     4261 tagcgccaac gatgcggttg agcgaagaat gcacgagtgc ggtattctgc tgcgcaagac
     4321 gaaaatcggc tcgccctacg tcgttgccgc ccttgacgaa ctacggttcg cgggaaagtg
     4381 ggagcgagtt gtcggttggg aagccaatgg cgggttttta accggcagcg atgtcgtttt
     4441 agccgggggc accttgagcg cgctgccgac gcgcgattcg acgttaccga tcctggccaa
     4501 tctattcgcc gcggccgagc ggaaagctag cctggccgaa ttgtgggatc tgctgccggc
     4561 tcggttcggc tgcgccggtt tgctcgacga ttttcctgtg gcggcgagcc aggcgattct
     4621 cgctaaactg attccgacca gcgggttgat cgaggtggag ttcggtgccg cgggagacgt
     4681 tttcgaccgc agtgtttcgg acaacactgc tgcgccgctt gccccctccg ctgcgagaga
     4741 ttggcaacaa gccaaaacga acttgatccg tttcttcacg cccgctcagg gcttcgatga
     4801 catcgctcga atcaatgtgc ttgatggcgt gcgcatctac ttccgcaacg gtgacgttgc
     4861 ccacatgaga ccatcgggca acgcgccaca gctgcggctc tatgcctgta gcgatttcca
     4921 agcacgcgcc gaggaaatcc gcgagctcgc tttgcgtgaa cccgatggca tcttgcgcca
     4981 attggagcga gcgtttacct gattaaccaa cttgggtgtt ttgcccttac aatcccaacg
     5041 ccggtctcac cttcgtcgcg aacagctcga tcgatcgtgt cacctggtcg tgactcaagc
     5101 cgccgaagct gaacatgccc actaagctgt cgggctttgc tgttgcgatc atcgcccgcg
     5161 ccttggcgac caccgtctgc ggactgccga tcagtgacgt gccccattca tcgaggcgct
     5221 tgatcgtcaa cccgccagga aaatacggaa agcgttcagt gaatttcggt aagtcgtcgg
     5281 ctgaaaccga atcaccgcgc cacacgttat ccgtggcgag ctgccaaaaa cgaatgagtt
     5341 ccggctccgc ttctttccac gcctgttcgt cggtttctgc gacgtagata tcgcggacca
     5401 tgatggcatc gccattcccc ggtaaattgt gtttggcccg ttcgtctcga taggatcgga
     5461 tcgcctgggc gcagttggcg agtggttggc gcgaaaccgc caggttccaa ccgcgtctgg
     5521 ctaccatggc agggctgtcg ggcgaagtgg cgccataata gatcggcggg tgcggacatt
     5581 gcagaggacg cgggcggcaa acgatatcaa cgtaattcca ggctctaccg gcgtaactga
     5641 aggtttcttc ggtccaggag cgcatcatga tctcgatccc ctcctcgaag cgcgcctgcg
     5701 ccgtgtcacg atcgagccca tgcttcaaat cgtcacgcgg gacaccgcgt cccaatccga
     5761 cattcaacct tccgccggtg aggatgtcca acatgccgca ctcttccgcg aggcgcatgg
     5821 ggtcgtagag cggcaggacg ttgaccagat tgccgagccg cagtcgtttg gttcgatgcg
     5881 ccaatgaggc gatcatcagg ttgggggatg caatcaggcc gtagtccgag tggtggtgct
     5941 cggcaaacca tgcctcgtca aagccaagcc gttcggtcaa gagaatttgt tcgagcagtt
     6001 cttcgaacgc ttgtttctcc gaaacccatg gccgtgactg attataacta aatgtcccaa
     6061 atcgcatccc tgaacctccc aagatgagtg ttcgtgctac tctaccgggt cacttgattc
     6121 aaagcgaccg cagggttagt tgacactcgc ttatcacaga acaataaact cgcaagcgac
     6181 tgcatcacca cgaatgtctc tgcggattct gcatagttca tcctttggtc cggttccatc
     6241 gagggagtca acaacggcaa acggataaac cgtgcatctg agttttccaa ggcagctcaa
     6301 ctccgctata gcgcaacatc aaatgatgcg ggttagagtg tactctggag gatttgatcc
     6361 gtcgaaatta ttgacccgaa tctcgcctca atgttaaaag tcggaagaat tgctgaggta
     6421 aacgcagaga ggagcagatc atggcaaagc tggttttggc ggcgggagtt ccacatccgc
     6481 cgaggctggt ttacgaaata gcgcaagcgc cgggtacaac ccgcggagaa gcgatgatga
     6541 agcaggttcg cgagcaggtg gaaaaggccg aacctgattt gattatcgag gttgacagcg
     6601 atcatttcgt caattttttc tacaacaatt taccatcatt ctgcgtcgga atggcggagg
     6661 aggccgaagg accgcaggag tcttggtgtc cgatgcctcg ttatacggtg cgtggcaacg
     6721 tgccgttggc caaggcactg ttgaggtacg gcgttcataa ccgattcgat cttgccgccg
     6781 cgcacgagct gcgtctggat cattcgttgg tggtgccgct gcactttctc aatcccggga
     6841 tggaactccc cgtggtgccg atctatacca acggctttgc ctcccccttg ccgctggcga
     6901 gccggtgcct tgctctgggc cgcatgttgc ggcgtttcat tgaatcctgg gaggggaaag
     6961 agcggattgc gctcatcgcc agcggttgtt tcgctatgga tgtcggcggc ccgctgcgcg
     7021 gctggaccga cacagactgg gcggaaacga tctcatcgct gctactcaaa ggacgttacc
     7081 gaactttggc tactcgtgcc accgaagatc gaatcaacgc cgcgggcaac aacagcggcg
     7141 agctgctcaa ctggatcacg gttagcgggg cggtcggcag gacccggcca ctgttcttgg
     7201 aaggcgacga aggcaatggt tatgcggtct ggaagtttga ctgacgtcta tgtggtttaa
     7261 cattccacaa ataacctgtg ccgatcggga agcttcgctg cctaagcgcc ttgcgaaaga
     7321 gcgggggtga gatgtgaccc tgaatagcgg ccaaccgaac aaaatgacaa gttttaacga
     7381 ctaaaattgc catgagcgtt tatcaactca acaaacttct atatctgacg gacaacgacc
     7441 ccgagtttcg caagcgcatg ttagcggatc cggaggcggc gctgagtgag ttcaaactaa
     7501 cttcagagga gcgcgacgcg ttgacttcag gcgcggtggg gaaactttat cagatggggg
     7561 ttcacacctt tctgctcaat cacctctatc gctaccagct cttcggcgtc aaccgtgaca
     7621 attacctgga gcggattcgc gaaggcatgc catacgatcc gcgcttcgag cttggcaaca
     7681 tgccggtgca acgattcatt accaaacaag attgaggcac ctaaatagcg atgccgtcga
     7741 accgcaacgt gcccgacggt tgagaacacc gggtcgctga agctcttgtc gatatcattt
     7801 tgacttcggg accgggtcgc tggcacgtga caatgtcgaa gtgagaatct tcaggttctc
     7861 gcttttctcg atgtagcgca cgaacaggta tttattttcc tgcgaatcca aagccgccgc
     7921 cgggacgtcg cctgcgcccg cttcgacctt tgacaagatg aagtagagat cgttgctgtt
     7981 caaagcgcgg gagaatcctt tttcgaattc ctcgatggtt ttcgcggtga tcacgttggc
     8041 aaagccggct cc
//
LOCUS       DASUTG010000055         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_8041, whole genome shotgun sequence.
ACCESSION   DASUTG010000055 DASUTG010000000
VERSION     DASUTG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8041"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF089_01205"
     CDS             complement(<1..317)
                     /locus_tag="VF089_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6172601.1"
                     /translation="MEYRQLGNAGVRVSVIGLGANRFGSKEVAQAEVDNIINRALDLG
                     VNFLDTADVYNEGRSEETLGQALKGRMDQVVLGSKFSFPRKTAPNSWGASRYHMMQAL
                     DASL"
     gene            complement(347..709)
                     /locus_tag="VF089_01210"
     CDS             complement(347..709)
                     /locus_tag="VF089_01210"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6172602.1"
                     /translation="MKKVYCAKDPLMIGHLRNVLGTYGIACVTKNLDLSTAAGELPPI
                     DCWPELWVEDEQLVRAKSILKKTMAPLKAVKRPWTCGGCAEEIEGQFTECWKCGRSRS
                     HEATRRENVVSLSHLRNR"
     gene            complement(729..1067)
                     /locus_tag="VF089_01215"
     CDS             complement(729..1067)
                     /locus_tag="VF089_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX6172603.1"
                     /translation="MSQREIKGYHAHIYYDPESREAAARVRAGLANFDVQLGSWHDEP
                     VGPHPKAMYQALFANEEFARVVPWLMLNRQDLTVLVHPSTGDAYGDHLDRSLWLGEVL
                     QLKTEVLRRG"
     gene            complement(1678..>2855)
                     /locus_tag="VF089_01220"
     CDS             complement(1678..>2855)
                     /locus_tag="VF089_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6172604.1"
                     /translation="WISEKLASLQSDGGTDLYKALVEAHRAIAAKQAAIKHVIVLSDG
                     LTDKADFRGLGRQMARDGITVSTVSVGSDADVQLMADIAKEGKGRGYVALDPQTIPQI
                     FTAETLLISRDLLVDKLFTPKIVAPVGPLKGISPSELPPLRGYVLTYPKPRSELLMTA
                     DKDPLLVSWRYGVGRVMAFTSDLSGRWGKEWVTWPGFPQWASQLARDTMRKLLETRMR
                     AELKPDGEAVKVVADLLSKDGKFLNHLRIRSNIATPSKRTLEQPLQQSAPGRYETKFV
                     PSERGIHLLTLYAEGEPGEPGQPLGTIPYIAPYPKEYRELKPNMALLSRLTEETGGEM
                     IDADKLDDGIARLYTPTPGKGTRGQETWWPLAGLGLALFLVDLVMRSWPRRAAQVQA"
BASE COUNT          604 a          834 c          746 g          671 t
ORIGIN      
        1 agactcgcgt caagggcctg catcatgtga taacgcgatg cgccccagga gttcggcgcg
       61 gtctttctcg gaaatgaaaa cttgctgccc aagacgacct gatccatgcg ccctttcaag
      121 gcttggccca aggtttcttc cgaccggcct tcattgtaaa cgtcggcggt gtccaagaaa
      181 ttaaccccaa gatcgagagc tcgattgatg atgttgtcga cttcggcttg cgccacttcc
      241 ttcgaaccga agcgattggc gccaagtcca atcacggaaa cgcgcacccc ggcgttgccc
      301 agctggcggt actccatgga aactccttca ggtgagtgat aaagacctat ctgtttcgca
      361 ggtgggaaag agagacgaca ttttccctgc gagtagcttc atgagaccga ctcctgccgc
      421 acttccaaca ctcggtgaat tgtccttcga tctcttcagc gcaaccgccg caggtccatg
      481 gcctcttgac cgccttcaaa ggcgccatgg ttttcttaag aatcgacttg gctctgacaa
      541 gctgctcatc ttcaacccac agttcgggcc agcaatcgat gggcgggagt tcgcccgccg
      601 cggtggaaag gtcaagattt ttggtgacgc acgcaatacc ataggtgccc aaaacgtttc
      661 tcaaatggcc gatcatcagg ggatctttgg cgcaatatac cttcttcatt atcgttccag
      721 cacttttatc agccgcgccg cagcacctct gttttgagct gcaaaacttc tcccagccaa
      781 agagaacggt caagatgatc tccataggca tcaccggttg atggatggac cagcacggtt
      841 aagtcttgcc gattaagcat caaccaagga accacccggg caaattcttc atttgcgaaa
      901 agcgcctgat acatcgcttt cggatgaggt ccaacgggct cgtcatgcca gctgccaagc
      961 tgaacatcga agttcgccag ccctgcgcgt acacgtgccg ccgcttcacg gctctccgga
     1021 tcatagtaaa tatgcgcgtg atagcccttt atttcccgct ggctcataac tgcgctcctt
     1081 gaggttccct aatcttgtac cgaattggct cggcggattc caaacggacc ttactatcgt
     1141 gcctatatac acgtatacgc tcatcgtcgc gaataaaaca ctaggatcag tgaaaagcca
     1201 aaccgcatca ccacggtttt ttcgtcgagc caatgccggg ggcgcagaaa gcgattaaca
     1261 acggctcaag cttttgtcga cggccggtcg gccgcagtgt tcttgtccaa gcattcagag
     1321 cagactccgg attgcttttt ccacaagccc ttgaagaaat gtctccggca taaaatcctt
     1381 cgacactgtt tgcacgccat tccgtttaac tcgccgaaag ttcgcttgca tatgccgcat
     1441 gtcaccggag ctatcgagat taacatgtga agaagttttt tcctctacaa ttgctgggag
     1501 tgaccttccc gttcgagctt cctatagcta attggatgcc cgccacttcg atgttgctag
     1561 ccttcgcgct ttgcttgccg ctgttgtgag gcgttcgata tcgtgatcat cgctcggttt
     1621 cggggtttta ccggtcgagc gaagatcgcg ctaccctcgc tagagtcaac ccgacgatca
     1681 agcctgaacc tgcgcggccc tgcgcggcca actgcgcatt accagatcga caagaaacag
     1741 cgctagcccc agacccgcaa gcggccacca ggtttcttga ccgcgcgtgc ctttgccggg
     1801 ggttggcgtg tagagacggg cgatgccgtc gtcgagctta tcggcatcga tcatctcccc
     1861 accggtctct tcggtaaggc ggctgaggag cgccatgttc ggcttcaatt cacggtattc
     1921 tttcggatag ggagcgatgt aaggaatcgt ccccagcggc tggcctggtt cgcctggctc
     1981 tccttcggca tagagcgtca gcaaatgaat gccgcgctct gatgggacga acttggtttc
     2041 gtaccgtccc ggcgcgcttt gctgaagagg ttgctcgaga gtccttttgc tcggtgtcgc
     2101 gatgttgctc ctgatcctga ggtgatttag gaacttgcca tctttagata acaaatcggc
     2161 cacaactttt accgcctcgc catcaggttt taattcggcg cgcattctag tttcaagaag
     2221 cttgcgcatt gtatcgcgcg ccagctggct ggcccattgc ggaaagccag gccaggtcac
     2281 ccattctttg ccccaacggc cgcttagatc cgaggtaaac gccattaccc gcccgacgcc
     2341 atagcgccac gataccagca gcggatcttt gtccgcagtc ataagcagct cggagcgcgg
     2401 tttcggatag gtgagaacgt agccgcgcag cggcggcaat tcgcttgggc tgatgccctt
     2461 cagcggccct accggcgcga cgatcttggg cgtgaaaagt ttatccacca gcagatcgcg
     2521 cgagatcagc aaggtttccg cggtgaatat ctgcggaatc gtttgcgggt ccaacgccac
     2581 ataacctctg cctttgcctt ctttggcgat atcggccatg agctggacgt cggcatcgct
     2641 gccgacggaa acggtcgaaa cagtgatacc gtcgcgcgcc atctgccggc cgagtccacg
     2701 gaagtccgcc ttatcggtaa gtccatcgga aagaacaatc acgtgcttga tggcggcttg
     2761 cttggcggca atcgcgcgat gcgcctccac caaggccttg taaagatcgg tgccgccgtc
     2821 tgactgcagc gatgcgagtt tttccgaaat ccatt
//
LOCUS       DASUTG010000056         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_897979, whole genome shotgun sequence.
ACCESSION   DASUTG010000056 DASUTG010000000
VERSION     DASUTG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897979"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1150)
                     /locus_tag="VF089_01225"
     CDS             complement(272..1150)
                     /locus_tag="VF089_01225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6172605.1"
                     /translation="MEIRQLNSFCWIVQSGSLKDAAKRCFLTPSALSLQIKALEQELA
                     LKLFDHKGRKLALTPQGEIFYVEAKKVLNSVEEAKEKTKNHAADFSGKISLAAPACLR
                     YFYLPSFARFRAAYPAIKLSILARSHADAISMARLGDADLAMGLFPHSFVDLDEIPLI
                     TPKLTLIIPRRGVRFTTRQIGLKELSEQPMVLLQPVTTTRAVIDGAFRKSNLKMRAEM
                     EASTCVEIKRYVASAIGIGIIHDICLEPDDNSRFRTVNLENIFPHPSARLIYNPAKSL
                     TASHKRFIEFIRFASS"
     gene            1235..2467
                     /locus_tag="VF089_01230"
     CDS             1235..2467
                     /locus_tag="VF089_01230"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6172606.1"
                     /translation="MAIPREVTKRARRVSLSDLCRVGRGTPAGDWFRRYWLAVGVAGE
                     LRDIPRAITVLGEELVLFRDEHGRIGLLGLHCPHRGSSLEYGDIENGGIRCPYHGWLF
                     DVFGRCLEQPAEPEGSIFCRKVKHLSYVVAEVGGLIFAYMGNEQESPPPLPQYRALVD
                     PNGQRSLEATRQYDYNWFNFIENGADPAHFSILHRAEPNDGTWRSWFFNFNDVPPFDA
                     VETSYGMKVVSRKPGPTAETEYVDEKSFALPSILQIGDTEFTHFKKPKEALAAGSHNA
                     HFMFVTPNDDDHFTLFTVNHYTGPDPEFFHKLEPSRRIAPKMERKEYDRRRYSPFRGS
                     VRSEDIMSQSTQGTIGERKEQLATSDKGVILLRKIILEAIETAQKGGAPKGIPLRDEP
                     ARVIDIDSFTGIRPRGVF"
     gene            2468..>2664
                     /locus_tag="VF089_01235"
     CDS             2468..>2664
                     /locus_tag="VF089_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter"
                     /protein_id="HEX6172607.1"
                     /translation="MKLSITMFARFLMSVFLGILLLVSGVEAAPLRVAYSAISGAMSS
                     LWVAQEGGYFKREGLDVELLY"
BASE COUNT          630 a          688 c          756 g          590 t
ORIGIN      
        1 agtagcgccg acaactccag aatgcctgtg aggtaaacaa tttgaagacg gtaaggagcg
       61 gcgggcggca acatcgcgga tatttcttcg gctctgatga gatggcccag agcggtaaag
      121 gccgaaaaac agcgagatgc cgattcgggc tcgaatcgcc gcgcccgttt tgaatctgag
      181 ggtccagtgg ctcagcccgg tcaagatcac atacgggccg acgagcagcg agaataacag
      241 aactaattgc accggtcacc agcagcgcgc atcagctcga ggcaaagcga atgaactcga
      301 taaacctctt atgcgaggcg gtcaggcttt tggcggggtt gtagatcagt cgcgcactag
      361 gatgaggaaa aatattttcg agatttacgg tgcgaaatcg gctgttgtca tccggttcga
      421 ggcagatgtc atgaatgatg ccgatgccga tggcactggc gacatagcgc ttgatctcga
      481 cgcaagtgct ggcttccatt tcggcgcgca tcttgagatt gcttttgcga aatgctccat
      541 cgatcactgc tctcgtggta gtgaccggct gcaacaacac catcggttgc tccgacagct
      601 ctttcaagcc gatttgtctg gtggtgaagc gcacgccccg gcgtggaatt atcagcgtca
      661 gcttgggcgt aatcaacgga atttcatcca gatcgacgaa agaatgcggg aacaggccca
      721 tggccaaatc ggcgtcaccc aagcgcgcca tcgaaattgc atcggcatgg ctcctggcca
      781 gaatgcttaa cttgatggcc gggtaggcgg cgcggaaacg ggcaaagctc ggcagataaa
      841 agtaacgcag gcacgccgga gccgcgagag agattttccc gctgaaatcg gccgcatgat
      901 ttttggtttt ttcctttgcc tcctcgaccg aattgagcac ctttttcgct tcaacgtaga
      961 aaatctcacc ctgcggcgtg agcgccagtt tgcgtccttt atgatcgaaa agcttcaagg
     1021 cgagctcctg ttcgagtgcc ttgatctgca gggataacgc cgaaggagtg agaaaacagc
     1081 gcttagcggc atctttgaga gaaccgctct gcacgatcca acaaaagcta tttagctgcc
     1141 tgatctccac aaaaatactt tagaaatatt aaactgatca ttaagtaaat taagattgac
     1201 atgacttcaa ctgcctggct aggatcttcg gtagatggca attccacggg aagtaacgaa
     1261 gagagcccga agagtttctt tgagcgacct ctgtcgcgtt ggccgcggca ctcctgcggg
     1321 cgattggttc cgccgctatt ggctggctgt gggcgtcgcc ggtgaactgc gggacatccc
     1381 tcgagctatc acagtcttgg gggaggaatt ggttttattt cgcgacgagc acggcaggat
     1441 tggcttactg ggtcttcact gcccgcaccg cggaagctct ctggaatacg gcgatatcga
     1501 gaacggcggg attcgctgtc cgtatcacgg ctggttattc gatgtcttcg gtcgctgttt
     1561 ggagcagccg gctgagccgg agggaagtat tttttgccgg aaagtaaagc atctctccta
     1621 cgtcgttgct gaagttggcg gactgatttt cgcctacatg gggaacgagc aggaaagccc
     1681 gccgccattg ccgcagtata gagctttggt cgacccaaac gggcagagat cgttagaagc
     1741 aacccgccag tatgattata actggttcaa tttcatcgag aatggcgccg acccggcgca
     1801 cttttcaatc cttcatcgcg cggagcccaa cgacggcacc tggcgcagct ggttttttaa
     1861 tttcaacgac gtcccgccat tcgatgcggt ggaaacttcc tacggcatga aagtggtctc
     1921 gcgcaaaccg ggaccgacag ccgagaccga gtacgtcgat gagaagagct ttgctctgcc
     1981 gagtattttg caaatcggcg ataccgaatt tacccatttc aagaaaccca aagaagcgct
     2041 ggcggcaggc tcgcacaacg cgcatttcat gtttgtgaca cccaacgatg atgatcactt
     2101 cacgctcttc accgtcaatc attacacggg tccggatccg gaattttttc acaagctcga
     2161 gccgagccgg cgcatcgcac cgaaaatgga aaggaaagaa tacgatcggc gccggtactc
     2221 gccttttcgc ggcagtgtcc gcagcgaaga catcatgagc caatcgacgc agggaaccat
     2281 cggcgaacgc aaagagcagc ttgcaacttc ggacaagggc gtcatcctgc tgcgaaagat
     2341 cattctcgag gcgattgaaa cggcacagaa aggaggagct ccgaaaggga ttcctctgcg
     2401 cgacgaaccg gccagggtga tcgatatcga ttcgttcacc ggcattagac ctagaggagt
     2461 cttttgattg aaactctcaa taacaatgtt cgcgcggttt ctgatgtcag tgtttctcgg
     2521 aatccttttg cttgtcagcg gcgtcgaagc cgcgccgctg cgggtcgcct acagcgccat
     2581 cagcggagcc atgtcgtcgc tctgggtggc gcaagaaggg ggctatttca aacgcgaggg
     2641 gctggatgtc gagttgcttt acat
//
LOCUS       DASUTG010000057         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_157139, whole genome shotgun sequence.
ACCESSION   DASUTG010000057 DASUTG010000000
VERSION     DASUTG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157139"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..900)
                     /locus_tag="VF089_01240"
     CDS             complement(187..900)
                     /locus_tag="VF089_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6172608.1"
                     /translation="MSVGNIIFEQIRSSGCLSYLLGCPEKKKCVVIDPELDKTDEYIE
                     LAKFFDSELLYAIDTHTHADHNSACKVLREKHGIKVIMHEATDAPYIDVAVKDGDVIS
                     FGTQSIKVIYTPGHTQDAMCLLCRDRIFTGDTLLIGGCGRTDLPGGSAEQQYESLRKL
                     RDLGDEIRVYPGHDYREAVSTLGDEKQKNTRMLFRSKEEFVQFMTSRKPPLPRKIEDA
                     LEWNRTPMQGTQRGMGEGI"
     gene            complement(911..1474)
                     /locus_tag="VF089_01245"
     CDS             complement(911..1474)
                     /locus_tag="VF089_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172609.1"
                     /translation="MAVQERKAESSFDRYIAEVRAVWGDGKDPQLPYKVAALMEKLFA
                     STSADDPWMAQIIRDGKPSRELYRDAEHGFIQMGHVHQSGHGNTPHDHGPCWVVYGAF
                     SGLTEITKYKRTDDGTQPGKATLKVDELHRLSPGTVKPYLPGEIHSTHAVEGPGVVFR
                     FLSYDLDKIERSRYNMEKETMIPVPAH"
     gene            1761..>2211
                     /locus_tag="VF089_01250"
     CDS             1761..>2211
                     /locus_tag="VF089_01250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6172610.1"
                     /translation="MSIEPQELRRIMGHFATGVTVITTQDKDGNPNGLTANAFMSLSL
                     DPPLVLISVDKGAQCYACFNLQNGFTVNFLSEDQEEVSRRFATKGIDKFAGLQWHFGQ
                     NGAAILDAALGYVECKITQCYDGGDHTIVVGEIVNANATDGRPLLFFK"
BASE COUNT          478 a          586 c          593 g          554 t
ORIGIN      
        1 gtcactttca acgcctagtg tcctgtctcc taccttggcg agccactgaa aaactctgtt
       61 tgcaggctgc tcaaaagacc tcagatagaa ggcgcgagaa gtccgatgag ctgaggcgta
      121 cgctgcagta cgttgaagcg agtcgggcga agcgcaacga agcctatgag tcttttcagc
      181 agcctgttaa atcccttcgc ccatccctct ctgtgtgcct tgcatgggcg tgcggttcca
      241 ttcgagagcg tcttcgattt ttcgcggcaa gggtggttta cgcgaggtca tgaactgcac
      301 gaactcctct ttactccgaa acagcatgcg cgtgtttttt tgcttttcat cgcccagagt
      361 tgaaacggct tcgcgataat catggccggg ataaacccga atctcgtcgc caaggtcgcg
      421 cagcttgcgc aagctctcat attgttgttc ggcgctgccg cccggtaagt cggttctgcc
      481 gcagccgccg atcaggagtg tatcgccggt aaaaatgcgg tcgcggcata aaaggcacat
      541 ggcgtcttga gtgtgcccgg gggtgtatat cactttgatg gattgagtgc cgaaggaaat
      601 gacatctcca tctttaaccg ccacgtcgat gtagggcgca tcagtggctt cgtgcatgat
      661 cactttgatg ccatgcttct cacgcaagac cttgcaggcg gagttatgat cggcatgggt
      721 atgggtatcg atcgcgtaaa gcagttccga gtcaaaaaat ttagcgagct cgatgtattc
      781 gtcggttttg tcgagctccg gatcgatgac gacgcatttc ttcttctcag gacagcccag
      841 gagataggac aggcagccgc tcgaccggat ctgctcgaag atgatattgc caacgctcat
      901 gttcgaccct tcagtgagca ggaacgggga tcatggtttc tttttccatg ttgtatcggc
      961 tgcgctcgat cttatctaaa tcgtagctca ggaaacgaaa gaccaccccc gggccttcca
     1021 cggcatgcgt cgaatgaatt tcgccgggca gatacggctt gactgtgccg ggggatagtc
     1081 gatggagctc atctacttta agagttgctt ttcccggctg tgtgccgtca tcggtgcgct
     1141 tgtatttagt gatttcggtg aggccgctga aagccccgta aacaacccag cacggcccat
     1201 gatcgtgcgg cgtgttgccg tgtcccgatt ggtgcacatg gcccatttgg atgaacccgt
     1261 gctcggcgtc tcgatagagc tcgcgcgagg gctttccatc tcgaatgatc tgcgccatcc
     1321 aaggatcgtc ggcactggtg ctggcgaaca gcttctccat caacgcggcc accttgtacg
     1381 gcagctgggg atctttgcca tcgccccaaa ccgcgcgaac ttcggcgatg tatctgtcaa
     1441 acgagctctc cgcttttcgt tcttgtaccg ccattgggca cctcctttga atttcatctt
     1501 taccatgttc cgtaaggaac cgtctagcag gttgctggga aaggtccctc cttcaactgt
     1561 gctcaggatg aactgagagc ccctggattc atgagcttga cgaaccatca atcgcatcgc
     1621 tcgaatccgt gttcgtgtta cgtactcgtt cgaattctcg atgacgtttc cacgaacacg
     1681 atcacgctga tttgaagccc tgattgacag cattaggttg actctggtag cagtaaaaaa
     1741 ttctcgaaaa aaaagcaatc atgtcgatag aaccacaaga gctgcggcgc atcatgggcc
     1801 attttgccac tggcgttacc gtgattacga cccaggacaa agacggaaat ccaaacggac
     1861 tcacggccaa tgcttttatg tccctttcat tagatccacc gctggtcttg atcagcgttg
     1921 ataaaggcgc gcagtgctat gcgtgcttta acttgcagaa cggtttcacg gtaaattttc
     1981 tcagcgaaga tcaggaagag gtgtcgcgtc gttttgccac caaagggatt gataagtttg
     2041 ccggcctgca gtggcacttc ggccagaacg gggcagcgat tctcgatgcg gcgctcggct
     2101 atgtcgagtg caaaattact cagtgctacg acggcggcga tcataccatc gttgtcggcg
     2161 aaatcgtcaa cgccaatgcc acggacggac ggccactgct ttttttcaaa g
//
LOCUS       DASUTG010000058         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_157199, whole genome shotgun sequence.
ACCESSION   DASUTG010000058 DASUTG010000000
VERSION     DASUTG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157199"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1667
                     /gene="icd"
                     /locus_tag="VF089_01255"
     CDS             261..1667
                     /gene="icd"
                     /locus_tag="VF089_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX6172611.1"
                     /translation="MAEYKVVQVPKDGAKITMGANKQLQVPDNPIIPFIEGDGTGRDI
                     WRASVRVFDAAVEKAYNGKKKIRWMEVYAGEKSFKQYNSWLHDETVPAFSEFLVGIKG
                     PLTTPIGGGMTSLNVGLRKLLDLYVCQRPVRYFNGVPSPLKHPEYVDMVVFRENTEDI
                     YTGIEFPNGTEANAKFKSYLKEAFPAEYAKIRFPNTAAVGLKPVSVEGTERLVRASIQ
                     WALANKRRNINFVHKGNIMKYTEGKFAEWGYALAKREFRNDVVTERESWILGNKEADA
                     NLSAEANAKMIDPGFDMMSPAQQNDIKKEVEEALKLWPTHGDGKWKKKLMLKDSIADV
                     TLQFVLIRPKDYDVIATLNLNGDYLSDALAAQVGGIGIAPGANINYDTGHAVFEATHG
                     TAPKYADLDKVNPGSVILSGEMMLRYMGWTEAADLIIKGIEGAVNAKTVTYDFERLMP
                     GSKLVSCSGFGDAIIANM"
     gene            1713..>2455
                     /locus_tag="VF089_01260"
     CDS             1713..>2455
                     /locus_tag="VF089_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX6172612.1"
                     /translation="MARKKIALIGAGNIGGSMAHLCLLKGLGDVVLFDVVEGLPQGKA
                     LDLSHAGPVEGFDGNVIGTNSYEDIAGADVCIVTAGIARKPGMSRDDLLGTNAKILTS
                     VADGIKKHAPNSLVIVVTNPLDAMVTLMKRATGFPKNRVVGQSGILDSSRYRSFIARE
                     LNVSVNSVAAMVLGGHGDDMVPVRSTCIVGGVPVEKLIPSKRLDEIEARVRNAGGEVV
                     ALLKTGSAYFSPASAAIRMTEAYLFDKKE"
BASE COUNT          591 a          627 c          695 g          542 t
ORIGIN      
        1 gtcgcgcggt catcctatag cccatgacgg cgcacggctc ttgtatatgt aggacgatcg
       61 tccaacaacg actctgaaag caacgtccgc aaatcggacg tacaggctcc gccgctctag
      121 gaattgacgc tgcctgctcc cgtagtcaat tccttgactc ccttaaccca atcagataac
      181 ttccaagcat tcaaaatttc tcgaacaatg cctaagggtt aaatcaaact cttaggcatt
      241 tttacaggtt gagggaggct atggcagaat acaaagtggt acaagtccct aaggacggcg
      301 cgaagatcac gatgggagcc aacaagcagc ttcaggttcc cgacaatccg atcattccat
      361 tcatcgaggg cgatgggacc ggccgcgata tctggcgcgc ctccgtccgt gtcttcgatg
      421 ccgcggtgga aaaggcttac aatggcaaga agaagatccg ctggatggaa gtttacgccg
      481 gggaaaaatc cttcaagcaa tacaatagct ggctgcacga cgagaccgtg ccggcatttt
      541 ccgagtttct cgttggcatc aaggggccgc tgacaacacc catcggcggc ggcatgacgt
      601 cccttaacgt cggactgcgc aagctgctgg atctttatgt ctgccagcgt ccggttcgct
      661 acttcaatgg tgtgccttcg ccgctcaaac atcctgaata cgtcgatatg gttgtgttcc
      721 gtgaaaatac cgaagacatt tacacgggca tcgagttccc caacggcacc gaagcaaacg
      781 ccaaattcaa gtcctatctt aaagaagcgt ttcccgcgga atatgccaag atccgttttc
      841 ccaatactgc tgcggtggga ttgaagccgg tttcagtcga aggcaccgag cgcctggtgc
      901 gcgcgtcgat tcagtgggcg ctggcgaata agcgcaggaa catcaatttc gtgcacaaag
      961 gcaacatcat gaagtacacg gaagggaagt tcgctgaatg gggctacgcg ctggcgaagc
     1021 gcgaattccg caatgatgtg gtgacggagc gcgagtcgtg gattctcggc aacaaggaag
     1081 ccgacgccaa tctcagtgcg gaagccaatg ctaagatgat cgatccgggt ttcgatatga
     1141 tgtccccggc gcagcaaaat gatatcaaga aagaagtgga agaggcgctc aagctttggc
     1201 ccacccatgg cgatggtaag tggaagaaaa agctcatgct caaagattcg attgccgacg
     1261 tcactttgca gttcgtgctg attcgtccga aggattacga cgtcatcgcg accttgaatt
     1321 tgaacggcga ttatctctcc gacgccctgg cagcgcaggt tggcggcatc ggcatcgccc
     1381 caggcgccaa catcaattat gacactggac atgcggtgtt cgaagcgact cacggaaccg
     1441 cgcccaaata cgccgatctc gacaaagtaa atccgggatc ggtcatactg tccggcgaga
     1501 tgatgttgcg ttacatgggc tggacagaag ctgctgatct gatcatcaaa ggcatcgagg
     1561 gcgccgttaa cgccaaaacg gtgacttacg atttcgaaag gctgatgccg ggctccaagc
     1621 tcgtgagttg ctcgggtttc ggggatgcaa ttatcgccaa tatgtgagcg agcggcgatt
     1681 gccgaatttt gttggattga cttgaggatt ttatggctag gaagaagata gcgctgatcg
     1741 gcgccggaaa tatcggcggt tcgatggccc acctgtgctt gctcaagggt ttgggtgatg
     1801 tggttctttt tgatgttgtc gagggattgc cgcagggcaa ggcgctcgac ttatcgcatg
     1861 ccgggccggt cgaaggcttt gacggcaatg tcattggcac gaatagctat gaagacattg
     1921 ccggagccga cgtctgcatc gtaacggcgg gaatagcccg caaacccggc atgagccgcg
     1981 atgatcttct cggcacaaat gccaagatcc tgacctcggt tgccgacgga ataaaaaaac
     2041 acgcccccaa ttcgctggtc attgttgtta ccaatccgtt ggacgcgatg gtcacattga
     2101 tgaagcgggc caccggtttt cccaaaaacc gggttgtcgg acagtccggc atcttggact
     2161 cctcccgtta tcggtcgttc attgcccggg aactcaatgt gtcggtgaat agcgttgcgg
     2221 ccatggtatt aggtggtcac ggtgacgaca tggttccggt tcgtagcact tgtattgtcg
     2281 gcggagtgcc ggttgaaaag ctgattccaa gcaaacggct cgacgagatc gaagccagag
     2341 tgcgcaacgc gggcggagaa gtggtcgcat tgttgaagac cggatcggct tatttttccc
     2401 ccgcatcggc cgccatccgc atgactgaag cttacctgtt tgataaaaaa gaaat
//
LOCUS       DASUTG010000059         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1936542, whole genome shotgun sequence.
ACCESSION   DASUTG010000059 DASUTG010000000
VERSION     DASUTG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936542"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..428)
                     /locus_tag="VF089_01265"
     CDS             complement(6..428)
                     /locus_tag="VF089_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005173568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX6172613.1"
                     /translation="MTEPNLMGGLIGGSLIGLAAVLLLAINGRIAGVSGILGGLLVPT
                     VKGDRAWRAAFLLGLIAGAALYGAWRGTLPLQFDVHGPVLVIAGVLVGIGTRLGSGCT
                     SGHGVCGLARRSPRSLLATVSFMAVAMLTVYLTRHILA"
     gene            complement(447..1505)
                     /locus_tag="VF089_01270"
     CDS             complement(447..1505)
                     /locus_tag="VF089_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172614.1"
                     /translation="MRHIGIKTLLLLLLGFISEVAWSAQVIEPLKEALQLHETGKIKE
                     AIVLYDRFIKTYPKRIEGYFNRGNAYYDLGQNERAVKDYSEVIRMSPKDSEAYFNRGN
                     AYRRLKQDRPALEDYNAAIKLNPQDSRAYINRGSIHFDARRYDLAVEDFSAAIRISPE
                     DADSYYNRGNSYLALNKNDEAIKDYTQALALEEKLADAFYNRGIAYSDSDKIDAAIAD
                     FTEVIKLNPKFSDAYYNRGLAAAKAGKYQAAVEDYSESIRLNGNDAEAPFNRGIAYLR
                     LARYDYAARDFSEVIRLQPRNAEAHVYRGIARIYQRKEKDADADFQKAFQLDPSLKKN
                     VQPLIEEAKAQINSEPAK"
     gene            complement(1511..2362)
                     /locus_tag="VF089_01275"
     CDS             complement(1511..2362)
                     /locus_tag="VF089_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172615.1"
                     /translation="MSAPHKILLLFVALVTPLGNIGWAGQAQNAFQEGVKHHQAGKFK
                     EAIAAYDRVIKASPDASEAYNNRGLAYHKLGQIGNALKDYDQAVKLNPNFTDALYNRG
                     NALLAQKQYERAIKDYDQVIKQNPNMPKAYDQRGQAYSGLGQYENAIKDFNQSQKLDP
                     KDPDIYYSRGLAYRRLGKPELAVQDYSEAIKLEPKNAEAYNNRANAYSLLGQFSHALR
                     DYDQAIKLDPRNARIYANRGIVKLRQGKGEDAQSDFNKAVQLEPSLKEQIQTITSDQS
                     APAVKKK"
     gene            2539..3597
                     /locus_tag="VF089_01280"
     CDS             2539..3597
                     /locus_tag="VF089_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172616.1"
                     /translation="MSTAAAVRTAQSVLADERIVSADSHIMEPEDLWEKNLPPSLKAK
                     FPKFPPRNTAGEKPGGWNPNARLDEMAVDGVSAEVLYPTHGLRLFALEDAELQEACFK
                     IANDWLIDYCAAAPGRLVGIPMISLYNIPNAIKELERCKKAGLVGSLIWQVPPEDLPF
                     TSDYYDPFWEASEALDMPPNLHILTGFNYSRFDRKGLDIYKATVNTKLYDAANTLFDI
                     VFSGVFARFPKLKIVYVENEVGWIPFFVHEWDKYFVRHSPKMPLPYMKKLPGEYVAEQ
                     VYATFFSDPAGGRLMEDFGQDTFMWSNDYPHAASTWPHSRDVIARELGHLPKDILRKV
                     VRDNVIKLYNLKIDGINN"
     gene            3634..4125
                     /locus_tag="VF089_01285"
     CDS             3634..4125
                     /locus_tag="VF089_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6172617.1"
                     /translation="MQASATAAKYDKDEKPKTKLFSLRTPYMKQGRITQLVAETENMW
                     IHTKINYEGGENEIHTHLDEDHAFIVLEGQMSVFDEKGGELKVGKYQGVMIPKGAYYR
                     YHNTGPDNLVVIRVGAGIKGKPQGGEEMRVRPDGKPLPAYSAENKTIEPIEDTRFFAD
                     FAG"
     gene            4217..4780
                     /locus_tag="VF089_01290"
     CDS             4217..4780
                     /locus_tag="VF089_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6172618.1"
                     /translation="MSRAEAKFAWETPPELWRGLKPSAAWKGSVVNLYIAPEPAGPMI
                     SVAQVRAFADRGLEGDRFFRESWAAAKRPDKAVSLIEEEVLDMAAAEHQITIAGDQTR
                     RNIVTRGVPLIDLLDRDFMIGNVVMRGIRLFEPCTHLEKVSHISGIFRALEHRSGLKA
                     AILSEGVIRTGDSITLCDCRNVVLDSR"
BASE COUNT         1103 a         1294 c         1318 g         1156 t
ORIGIN      
        1 cgctttcatg ctaaaatatg tcgggtcaag tagacggtca gcatagccac tgccatgaaa
       61 ctcacggtgg cgagcagcga ccgaggtgaa cgccgagcta aaccgcagac gccgtgtcct
      121 gaggtgcacc cggatccgag gcgcgtgccg atgccgacga gcaccccggc gatgaccagc
      181 acaggcccgt gaacgtcgaa ctgcaacggc aaagtacctc gccacgcgcc atacagggca
      241 gcgcccgcga tcaaacccaa gagaaacgcg gcgcgccagg cgcgatcacc tttcactgtg
      301 gggaccagca aaccaccgag aatgccggat acaccagcaa tccgcccatt gatggcaagc
      361 agcaggacgg cggccaggcc gatcaaggag ccgccgatca gtccgcccat caggttcggt
      421 tccgtcatca catgctcctg aagccgttac ttcgccggct cgctattgat ttgcgccttg
      481 gcttcttcga tcaggggctg aacgttcttt ttcaaactcg gatcgagttg gaaggccttt
      541 tggaaatcgg cgtcagcgtc tttttctttt ctttgataga tgcgcgctat gccgcgataa
      601 acgtgagctt cagcattgcg cggttgcagg cggatcactt cgctgaaatc tctcgctgca
      661 taatcgtatc gcgccaagcg caggtaggcg atgccgcgat tgaatggcgc ttccgcatcg
      721 ttgccattga ggcgaatcga ttcgctgtaa tcttcgaccg ccgcctgata ctttccagcc
      781 ttggccgccg ccagcccccg attataataa gcgtcgctga actttggatt gagttttatc
      841 acttcggtga aatcagcgat ggcggcgtcg attttgtccg agtccgaata cgcaatgcca
      901 cgattataaa aggcatcggc cagcttttct tctaacgcga gcgcctgcgt ataatccttg
      961 atcgcctcgt cattcttgtt gagagccaga tagctattgc cgcgattgta ataagaatcg
     1021 gcgtcttccg ggctaatgcg gatcgcggcg gagaagtcct ccaccgcgag gtcatagcgg
     1081 cgcgcgtcga agtgaatgct gccgcggttg atgtaagctc ggctgtcttg cgggttcaat
     1141 ttgatggcgg cgttgtaatc ctcgagcgcc ggccgatctt gtttcagccg ccgataggca
     1201 ttgccgcggt tgaaataggc ctcgctatct ttgggcgaca tgcgaatcac ttcgctgtaa
     1261 tcttttaccg cgcgctcatt ctgtcccaga tcgtaatacg cgttaccgcg attgaaatat
     1321 ccttcgatcc gcttcggata agttttgatg aacctgtcat agaggacgat ggcttctttg
     1381 atttttcccg tctcgtgcaa ttgcagcgcc tctttaagcg gttcgataac ctgagcggac
     1441 cacgcgactt cagaaatgaa tccaagcaac aacaatagaa gagtcttgat tccgatatga
     1501 cgcatggcgg ttactttttc ttgactgccg gagcgctttg atcgctggtg attgtttgga
     1561 tttgttcttt caagctcggt tccagctgca ccgctttatt gaagtcgctc tgggcatcct
     1621 cgcccttgcc ctggcgcagc ttgactatgc cgcggtttgc gtagatccgc gcgtttctgg
     1681 gatcgagctt gatggcttga tcgtagtctc tgagcgcgtg actgaactgg ccgagcagag
     1741 aataggcatt ggcgcgattg ttataggcct cggcattctt tggttccaat ttgatggctt
     1801 cggaatagtc ctgaaccgcc agctcaggct tgccgagccg ccggtaggca agcccacggc
     1861 tataatagat atcggggtct ttgggatcga gtttctgcga ttggttgaag tccttgatcg
     1921 cgttttcgta ttgtcccagg cctgagtaag cttggcctct ctgatcatag gctttcggca
     1981 tgtttggatt ctgcttgatg acttgatcgt aatcctttat cgcgcgctca tattgtttct
     2041 gcgccaacag agcgttccct cggttgtaca atgcgtcggt gaagttagga ttgagtttaa
     2101 ccgcctggtc gtaatccttg agggcgttgc cgatctgtcc gagcttgtgg taggccaggc
     2161 cgcggttatt gtaggcctca ctggcatcag gggaggcctt aatgacgcga tcgtaagccg
     2221 caatcgcttc tttgaatttg cccgcctgat ggtgtttaac gccttcctga aatgcattct
     2281 gcgcttggcc ggcccagcct atgttaccca agggcgttac tagcgcgaca aacaacaata
     2341 agattttatg cggtgccgac atcaaggccg aatttagcac cgaacctaca attgaacaat
     2401 ttcatcccca aacttttttc gtcgacgaac tcacagggaa ccgctgttaa tagcgcagcg
     2461 tgcgatttgg gttagctgga caaagcactc tagatgaaat aaaagtactc aataaatcaa
     2521 aaagcgaggg aaaaccttat gagcacagcc gccgcggtcc gaacagcgca gtcggtactt
     2581 gccgatgaac gtatcgtctc ggcagattcc cacatcatgg agcctgaaga tctctgggaa
     2641 aaaaatctgc ccccttctct aaaagccaaa tttcccaaat ttcctccgcg caacactgca
     2701 ggtgaaaaac ccggcggatg gaatcccaac gcccgcctcg acgagatggc ggtcgatggc
     2761 gtcagtgccg aagttctcta cccgacgcac gggcttcggc tgttcgcgtt ggaagacgcc
     2821 gagctgcaag aggcgtgctt taagatcgcc aatgactggc tgatcgatta ctgcgccgcg
     2881 gcgccaggcc gcctggtcgg aattccgatg atctcgcttt acaacattcc aaacgcgatc
     2941 aaggaattgg agcgttgcaa gaaagccggg ctggtcggca gcttgatctg gcaagtgccg
     3001 cctgaggatc tgccgtttac cagtgactac tatgatcctt tctgggaagc ctcagaggct
     3061 ttggatatgc cgcccaacct gcatattctg accgggttta actacagccg gttcgatcgc
     3121 aagggcttgg atatttataa agccaccgtc aacaccaaac tctatgatgc cgcaaatact
     3181 ttgttcgaca tcgttttcag cggagttttc gcacgatttc ccaagctgaa aatcgtttat
     3241 gtcgaaaatg aagtcggctg gattccgttc ttcgttcatg aatgggacaa gtatttcgtg
     3301 cggcatagcc caaagatgcc gttgccctac atgaaaaagt tgcccggcga gtacgttgcc
     3361 gaacaggttt atgcgacctt ctttagcgat ccggctggcg gccgtctaat ggaagatttt
     3421 ggccaagata ccttcatgtg gtccaacgat tatccgcatg ctgcttcgac ttggccgcac
     3481 tcgcgagacg taatcgcccg cgagttgggg catctgccga aggacatttt acgcaaagtc
     3541 gtccgcgaca acgtgatcaa gctgtataat ctcaaaatcg acggcatcaa taattgacaa
     3601 ttaacccgga tatcacggaa aaggagaatc actatgcaag caagtgcaac cgcggcaaaa
     3661 tatgacaaag acgagaagcc gaaaacgaaa ctgttttccc tcagaactcc gtacatgaag
     3721 cagggacgca tcacccagct ggtggcggag accgagaata tgtggattca caccaaaatt
     3781 aactacgagg gcggtgagaa tgaaatccac acccacctcg acgaggacca tgctttcatc
     3841 gttctcgagg ggcagatgag cgttttcgat gaaaagggcg gtgaactcaa agtcggcaag
     3901 taccaaggtg tcatgattcc caaaggcgcc tattatcggt atcacaacac tggccccgac
     3961 aatctcgtag tcatccgtgt cggcgccggc atcaaaggca aaccgcaagg cggcgaggag
     4021 atgcgagtgc gccccgacgg caagccgctg ccggcatact cggcagagaa caaaacgatc
     4081 gagcctatcg aggatacccg attcttcgcg gattttgcgg gatagacccc aacggcaacg
     4141 cattacagtt taaaagcaat gcgaacgatg gccactgata ctcatcagtg gccatcgtca
     4201 tttacggagt aattttatgt cacgagccga agcgaaattt gcctgggaga cgccaccgga
     4261 actatggcgc ggcttaaagc ccagcgctgc ttggaagggc agcgtggtta atctgtacat
     4321 cgctccagag cccgccggtc cgatgatttc ggtggcgcag gtgcgggcct tcgccgatcg
     4381 cggcttggaa ggcgatcgtt tctttcgcga gtcttgggca gcggcaaagc gccccgacaa
     4441 agctgtcagt ctgatcgaag aggaagttct cgatatggcg gcggcggaac atcaaataac
     4501 aatcgctggc gaccagacca ggcgcaacat tgttacccgc ggtgtgccgt tgatcgatct
     4561 gctcgatcga gacttcatga tcggcaacgt agtcatgcgc ggcattcgcc tgttcgagcc
     4621 gtgcactcat ctggagaagg tttcacatat ctctggcatt tttcgcgcgc tggagcaccg
     4681 cagcggtctg aaggcggcga ttctcagcga gggtgtgatt cgcaccggag attcgatcac
     4741 gttatgcgat tgccgaaatg tggttctgga ttccaggtga tctgcaacca ttggtttcaa
     4801 actatgagtt tatccagggt accgtactgc taagtggagg ttgatggttc tgctagagtc
     4861 cgtccttccc g
//
LOCUS       DASUTG010000060         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1047192, whole genome shotgun sequence.
ACCESSION   DASUTG010000060 DASUTG010000000
VERSION     DASUTG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047192"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VF089_01295"
     CDS             <1..880
                     /locus_tag="VF089_01295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6172619.1"
                     /translation="MESLGSRVVPVDVLPRSADLSLLAIQTPSRIMNVTDEAPFRELC
                     RLEHPTHIFHAAHPPRDDSPEVLNFCYRAMTNILDAARELKVKSVVYSSSGALYGQLR
                     KNDHAAIKEDDPVAIYPTFFYRSAKIMSEWMGDFYKQKFAVPFVALRYSSVYGPGLGR
                     GIPLELKKGLLRQPCRPYLTRLPDDLIYIDDVLDAVRRALFAEKPLSRAYNIAFDKTF
                     DNRDLEDSIRKALPEISFEIGKHPHTATVGPHRDRDPLDITRAKEELDWTPRIDLEDG
                     IARIAAWLSSHANRLK"
     gene            925..2034
                     /locus_tag="VF089_01300"
     CDS             925..2034
                     /locus_tag="VF089_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6172620.1"
                     /translation="MTAFPMAKNVTAIRWLFKAGFVVHILLAVVEQSAAAGRSLSSSE
                     LFFYQGADREQILLEGAKKEGQLVFYNSHTWFKSVAQEFEKKYPFVKVSVWRADGADV
                     MKRVLEESLAGRFLADVVESTEANIGGLHKRQLLQEHHSPELRFFKDEVLGKGKRGVF
                     YWADRETYISLGFNTTHVSIEEAPKRTQDLLAPRFKGKISFAGGATAARWVGAVIDAL
                     GRDFIEKLSRQDVTVHKLSGAALANLVVTGEVPLSPTIFDSNMFVAKNKGAPVEWRPM
                     QPVVANVGYSSVVAKAPHPHAALLFIDYLHSKEGQQVVVKGGLSSPRDDVDSLVPQKF
                     KKTYLEAKYPLDEYEKKLDEWEDLIQRLFIRKGKL"
     gene            2047..3126
                     /locus_tag="VF089_01305"
     CDS             2047..3126
                     /locus_tag="VF089_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6172621.1"
                     /translation="MALKRANRRAGVPLVVAICLLATVNVFAASAPMSDAQIALYQGS
                     DREKVLIEGAKREGQLTFYNSHTWFRSYVKEFEKKYPFIKVSEWRSDSKNLLSRAMAE
                     FGSTRSLVDVIETTAEAMGVMKRNGMFQEYFTPEARYYPDDVKVKGKSGLYYLGDRET
                     YNSLGFNTTLIAPNEAPKTRNDLLDPKWKAKMAIAGTSTGVRWVGSTLDTLGREFLNK
                     MAAQELTVQNMSGAALAGLVASGEVPLSPTIFDADVTVAKQKNAPIQWRALDPVVTTV
                     GYSGLTSKSSHPHAALLFLDYLHGKEGQQLMMKGGLWSPRYDIGSLDQKFEKLYLDSK
                     YSLDELEKKLVEWENLMRQLFVRRK"
     gene            3298..>4319
                     /locus_tag="VF089_01310"
     CDS             3298..>4319
                     /locus_tag="VF089_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172622.1"
                     /translation="MKSRHSPHPDLILHSGKIRTFAGNGSISEAIACAGGRIVATGSS
                     EEIRRLAGPDTESFDLKGKTAIPGLTDTHVHLSEKGTAEMELVDCRDFYVDVNSVSDI
                     LQRLANAAARAPKGSWIVAHGSPMQDFRIEDKRFPDRHDLDRVVPDHPVSISFGAHVT
                     IANTPALAAAKITRDTPDPAGGHIRHDPQTGEPTGELHERAQLIVKKVAPEFNYLQLK
                     DGIVFALDQCLQRGVTTVHDIVRYAEPVRAYQELYKEGRMHARVSILPRVIESMIESK
                     SLIELGLITGFGNDWLRIGGVKMSIDGGITGRNACFHEPYENDEHNHGIIRIQQDELN
                     HTVQVC"
BASE COUNT         1020 a         1132 c         1188 g          979 t
ORIGIN      
        1 tatggaatcg ctcggcagta gagttgttcc tgtcgacgtc ttgccgcgct cggcggatct
       61 gtcattgctg gcgatccaga ctcccagtcg catcatgaat gtcaccgatg aagcgccctt
      121 tcgggagctc tgccggttgg aacatccaac gcatattttt cacgccgctc atcctccgcg
      181 cgatgatagc cccgaagtcc tgaatttttg ctatcgtgcg atgaccaaca tcctcgacgc
      241 tgcccgggaa ctaaaggtca aaagcgtggt gtactccagt tccggagcgc tttacggcca
      301 gctacggaaa aatgatcacg cagcaataaa agaagatgat cctgtggcga tctacccgac
      361 cttcttttac cgctccgcta agatcatgtc ggaatggatg ggcgactttt acaaacaaaa
      421 attcgcagtt ccattcgtcg ccctaagata ctcgtcagtt tatggaccgg gactcggcag
      481 aggtattccg ctggagttga agaagggtct cctgcgccag ccctgccggc cttatctaac
      541 ccgcttgccc gatgacttga tctatattga cgatgtctta gacgctgtgc gccgcgcgct
      601 cttcgccgaa aagccgttga gccgcgctta caacatcgcg ttcgacaaaa ctttcgacaa
      661 ccgagatctc gaggactcga tccggaaagc gttgcccgag atctcttttg agatcggcaa
      721 gcatccgcat acggcgacag tgggaccgca tcgcgatcgc gatccattgg atatcactcg
      781 ggccaaggaa gagctcgatt ggacacccag aattgatctg gaagacggga ttgcgcgaat
      841 cgccgcctgg ctaagtagcc atgctaatag gctgaagtaa actggcaacg acgcatcgtc
      901 actattcggt caggaggaaa aaacatgact gcctttccga tggcaaagaa cgttactgcg
      961 attcggtggc tgtttaaggc tggctttgtt gtgcacattc tcctggcagt tgtagagcag
     1021 tcagccgcag ccggcagatc gctcagttcc tcggagctgt tcttctatca gggcgcggat
     1081 cgagaacaga ttctcctcga aggagcaaaa aaagaagggc agctggtttt ctacaattcc
     1141 catacgtggt tcaagtccgt cgcgcaggag tttgagaaga agtacccctt cgtgaaggtc
     1201 tcggtctggc gcgccgacgg cgcggatgta atgaagcgcg ttctagagga aagtttggcc
     1261 ggccgtttcc tcgctgacgt cgtcgagagc accgaagcca acatcggcgg tctgcacaaa
     1321 cgacaactgc tccaggaaca ccactctccc gaacttcgtt tttttaagga tgaagtcctg
     1381 ggcaagggta agcgcggggt attctactgg gctgaccggg agacgtatat cagtctgggc
     1441 ttcaatacaa cccatgtatc cattgaggaa gcccccaagc gcacgcagga ccttcttgcc
     1501 ccgaggttta aaggcaagat ctcctttgcc ggcggcgcca cagcggcaag atgggtaggc
     1561 gccgtcatcg acgcgctcgg cagggatttt atcgaaaagt tgagccgcca ggatgtcacc
     1621 gtccataaac tctccggcgc ggcgctcgcc aacctggtgg taactggaga agtgccgctc
     1681 tcgccgacaa tttttgactc caatatgttt gttgccaaga acaagggagc tcccgtggaa
     1741 tggcgaccca tgcagccggt ggtggctaac gtcggctact ccagcgttgt cgccaaagcg
     1801 ccgcatcccc atgccgctct gctattcatt gactatcttc attccaagga aggtcagcag
     1861 gtggtggtga aagggggtct cagctcgccc agagacgatg ttgactctct ggtgccacag
     1921 aagttcaaga agacctatct tgaagctaaa tatcctttgg atgagtacga aaaaaagctc
     1981 gatgagtggg aagatctcat tcaacggctt tttatcagaa aaggtaagtt ataaagggag
     2041 gcaaccatgg ccctgaagcg agccaatcgc cgagccgggg ttccacttgt cgttgctatc
     2101 tgcttacttg cgacggtcaa cgtatttgcg gcgagcgcgc cgatgtcgga tgcacagatc
     2161 gcgctctatc agggaagtga tagagaaaaa gtgctcatcg agggagcgaa gcgagaaggg
     2221 cagctcacct tttacaactc ccatacgtgg tttcgaagtt acgtcaaaga attcgagaag
     2281 aagtaccctt tcatcaaggt ctcagagtgg cgcagcgatt caaagaatct gcttagccga
     2341 gcaatggcag agttcgggtc tactcgttct ttagtcgatg tcattgaaac taccgctgaa
     2401 gccatgggcg tgatgaaacg gaacggcatg tttcaggagt attttacccc agaggcgcgt
     2461 tactatcctg acgatgtcaa agtaaaaggg aagagcggac tttactatct tggcgatcgc
     2521 gagacgtaca atagtctagg cttcaatacc acgctcatcg ctccgaacga ggcgcctaag
     2581 acgcgtaacg atctcctcga tccaaagtgg aaggcgaaaa tggccatcgc cggaacatcc
     2641 accggcgtta gatgggttgg tagcacgctg gatacgctgg gtcgtgagtt tctgaacaaa
     2701 atggccgcac aggagttaac ggtgcaaaat atgtccgggg cggctttggc gggtttggta
     2761 gcatccgggg aagttccttt atcgccgacg atcttcgacg cggatgtgac cgttgcaaaa
     2821 caaaaaaatg cgccgatcca atggcgtgct ctcgatccgg tcgtaaccac ggtcggctat
     2881 tccggcttaa cgtccaaatc ctctcatccg catgcggctt tgctctttct tgactacctg
     2941 catggcaagg agggacagca gctaatgatg aaaggcggcc tttggtcgcc tcgctatgac
     3001 atcggatcgt tggatcagaa gtttgagaaa ctctaccttg acagcaaata ctcgctcgat
     3061 gagctcgaaa agaagctcgt agaatgggaa aatttgatgc gacaactttt tgttagaaga
     3121 aagtagattt cgatcgagtt tcgcaactaa ccggcggcgc tgcgtccatc ggtcattatt
     3181 cagagttgcc ggatctgctg atctcagatg ctgttgactc cgattccgaa gcaacttatg
     3241 gctagatgat gccgcattct acactggtgc tgcattctaa aggaggaaaa gctttcaatg
     3301 aaatcacggc attcaccgca tcctgatttg attctgcaca gcggcaagat tcgcacgttt
     3361 gccggtaatg gttcgatctc tgaggcgatc gcttgtgccg gcgggcgcat cgtggccacc
     3421 ggatcgtctg aggagattcg gcgattggcg gggcccgaca cggagtcctt cgatctcaag
     3481 ggtaaaactg ccattcctgg attgacggat acgcacgttc atctttcaga gaagggcaca
     3541 gcggagatgg agttggtcga ttgtcgcgat ttctatgtcg atgtgaactc cgtctctgat
     3601 attttgcagc gccttgccaa cgcggccgcg cgggctccga agggctcctg gatcgtcgcc
     3661 catggcagtc cgatgcaaga cttccgcatt gaggacaagc gctttcccga ccgccatgat
     3721 cttgaccgcg tagtgcccga tcatccggtt tccatttcct tcggcgctca tgtcaccatc
     3781 gccaacacgc cggctctggc cgcggcgaag attacacgcg atactcccga tcccgccgga
     3841 gggcacatca ggcatgatcc gcaaaccggg gagcccaccg gcgaattgca cgagcgggcg
     3901 caattgattg tcaaaaaagt cgccccggaa tttaattatc tgcaattgaa agatggaatt
     3961 gtcttcgcgc tggatcagtg tctccaacgg ggcgtgacca ccgttcatga catcgtccgc
     4021 tacgccgaac cggttcgggc ttatcaggaa ctgtacaaag aaggccgcat gcacgctcgg
     4081 gtgagtatcc tgccgcgggt gattgagtcg atgatcgaat cgaagagcct catcgagctt
     4141 ggccttatta ccggcttcgg caatgactgg ctacgcatcg gcggcgtcaa gatgagcatc
     4201 gacggcggga tcaccggacg caatgcttgt tttcacgagc cttatgaaaa cgatgaacac
     4261 aaccatggca tcataaggat tcagcaagac gagctgaatc ataccgtgca ggtatgtca
//
LOCUS       DASUTG010000061         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1788761, whole genome shotgun sequence.
ACCESSION   DASUTG010000061 DASUTG010000000
VERSION     DASUTG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788761"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF089_01315"
     CDS             <1..530
                     /locus_tag="VF089_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX6172623.1"
                     /translation="IEETLGTMGSYIALAFAASQFVAYFGWSNLGLILAVSGADLLRV
                     SGLSGIPLIILFIFVCAVIDLFVASASAKWAVIGPVFVPMLMILGFSPEMTQGAYRIG
                     DSFTNIITPLMPYMPLIIAFAQKYDPRIGLGTLLTLMLPYSVSFALGWTLLLIVWLLL
                     GIPLGPGSGIVYPPT"
     gene            633..1391
                     /locus_tag="VF089_01320"
     CDS             633..1391
                     /locus_tag="VF089_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018952529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6172624.1"
                     /translation="MTNLASKQSRTVRGSGHCRIGTSGFHYKHWKGIFYPENLSSKQW
                     FAYYASQFDTVELNNTFYRLPPEAYFDAWRAGAPKGFCFAVKFSRYGSHVMKLKNADD
                     TIRRFTERAERLADHLAPILVQLPPKWDVNVERLRDFLAAAPRRHRWTLEFRDPRWLT
                     DEVYEVLRRYKAALCIHDMIPEHPREITADWVYIRFHGGRYDGNYDDATLGVYAREIE
                     GYLKSGLDVYVYFNNDLHGHALKNAATLKRLLAR"
     gene            1499..2503
                     /locus_tag="VF089_01325"
     CDS             1499..2503
                     /locus_tag="VF089_01325"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172625.1"
                     /translation="MNNRLILPCAVFITLALFCAAPAQEKKLHKIRLGGGSASATQMT
                     MWLAKEGNYYEKHGLDAETISIPGSSLALQAMLAGELPIIQLGGAASIQANFSGADTV
                     IIATIVRKFLFSIFARPGIEKMEDLRGKVFGATRFGTLSDLASRFALRAYGIEPERDI
                     TMVQTGGPAETVTAMAAGKVHAAAITPPATLQARKVKLKELLDMSKLDAEYHINGVVT
                     TRRYLKTNEDIVRRFLRAYIEGAARAQKDKNFALKAMSKYFRTDDREILEESYNAVIK
                     SNFAIPPYPSISGIASLLQGLEKQNPKAKGAKPEDFSDSRLVRELDQSGFIKSVLPER
                     "
     gene            3113..>5110
                     /locus_tag="VF089_01330"
     CDS             3113..>5110
                     /locus_tag="VF089_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX6172626.1"
                     /translation="MKNSLKSLTSAVSGCVNRAFRERGEQYYLDGAVNIIEGSAWRVA
                     ATVQGTRRYEVIIVRARDFFQVSCSCPLYDRELVTCKHIWAVFLAAEQSRFLDGLGDS
                     APGEIREEIPGLFNYHQAEGSPTSRLPEVSWKQQLQRLSGSMAAQDRRFNLDAGPERY
                     FLYVIDMNGCVDSERLTIHVVQRTRKADGTWGKLKTQKIRFNEIATLPDPADRRILAL
                     LAGAREETNYGYSPYYDSVQSTFSLAEPSWDLVLPLICATERCVLGLSTDPDQQSLVW
                     GEGEHWEFGMEMTLDESAGTYSVQGLLRRGDVEMDLQRPQLIIAGGLIVYDGRIARLR
                     DFGAFSWIALLRDHGPISVPKTEGDEFVNEILRMPRRPRLKLPHEFSFEEVTCRPNPA
                     LAISSKTERKAANARLLANLSFEYDGTLVPHRYPGQAIHQQNRRRLINRDFAVEDAAV
                     TRLKELGFRRPHWPGSGSEFEFSPDRLPNVVRALSGEGWRVEAEGKIYRSASALRLNV
                     KSGVDWFELEGGAQFDQVQMALPKLLRAIKRGEETVRLDDGTLGILPEDWLEKYRLLA
                     GVGSMHGDQLRFSRKQVGLIDALIASEENVSVDGGFERVRQELYSFAGVEAADPTPDF
                     SGHLRDYQREGLGWLAFLQRFGLGGCLADDMGLGKTVQVLAL"
BASE COUNT         1138 a         1384 c         1405 g         1183 t
ORIGIN      
        1 tgatcgaaga gacgcttgga accatgggga gctacatcgc gctggcgttt gcagccagcc
       61 agtttgtcgc ttattttggc tggtccaatc tcggcttgat actggccgtc agcggcgccg
      121 accttttgcg ggtatcaggc ctgtcgggga ttccccttat catccttttt atctttgtct
      181 gcgccgtgat cgatctgttc gtcgccagcg cctccgctaa gtgggccgtc atcggacctg
      241 tgtttgtgcc gatgctgatg attctaggct tctctcctga gatgacccaa ggcgcttatc
      301 gtatcggcga ttctttcacc aatattatca cgccacttat gccatacatg ccgctgatta
      361 ttgcgttcgc ccaaaaatat gacccgcgca tcggtcttgg aactttgctg acactgatgc
      421 tgccttattc ggttagcttc gcgttaggct ggacgctgct gctgatcgtc tggctgcttc
      481 taggaattcc gctcggtccc ggctccggca ttgtttaccc gccaacgtga tcatgtcttg
      541 aagatctctg ttggctcaac gattgcggaa gttacgctat ccagagtgat gatcgcccac
      601 agcacattca tctaagtaaa ggcagtttcg cgatgacaaa cctcgcatcg aagcaatcgc
      661 gaaccgttag aggctccggt cactgccgca taggcacctc cggctttcat tacaagcact
      721 ggaaaggaat cttctatcct gagaaccttt cttccaagca atggtttgct tactatgcct
      781 cgcagttcga cacagttgag ctcaataaca ccttttaccg gctgccgccg gaagcttact
      841 tcgatgcctg gagggccggg gctccgaagg gattttgttt tgccgttaaa tttagccgct
      901 acggttcgca tgtgatgaaa ctaaagaatg ccgacgatac gatccggcga ttcaccgagc
      961 gcgccgaacg cctggcagat catttggctc cgattctagt ccagctgccg cccaaatggg
     1021 atgtcaacgt cgaacgatta agagactttc ttgcagccgc tccccggaga catcgctgga
     1081 cgctggaatt tcgcgatccg cgttggttga cagacgaggt ctacgaagtg ctgcgtcgct
     1141 ataaagccgc gttgtgcatc catgacatga tccccgagca tccccgagag attactgcag
     1201 attgggttta catccgattt cacggcggcc gttacgatgg caactacgat gacgcgaccc
     1261 ttggtgttta cgcccgagaa atcgaaggtt atctaaaatc cggactggat gtttacgtct
     1321 acttcaataa tgatttgcat ggccatgcgc taaaaaatgc cgcaaccctg aaaagactat
     1381 tggcaagata ggtcgatcgc gcatgggctt ggcttcagct catccaaccg gatcgcttga
     1441 cacgtaggct gctgcctctc tattttggca ccactcgagc tgcgaaaagg agcgccacat
     1501 gaacaatcgc ctaatattgc cctgcgccgt gttcatcacg ttagctctgt tttgcgctgc
     1561 gccggcgcaa gagaaaaaac ttcacaagat caggctgggc gggggctcgg cgtctgcaac
     1621 gcagatgacc atgtggctcg ccaaggaggg aaattattac gagaagcacg gcttggacgc
     1681 ggagaccatc agcatccccg gcagttccct ggctcttcag gcgatgctcg caggtgagtt
     1741 gccgatcatc cagctcggcg gcgctgcctc aatccaagcc aacttttccg gggccgacac
     1801 tgtgattatc gcgaccatcg tgcgtaagtt cctattttcg atctttgccc gcccgggcat
     1861 cgaaaaaatg gaggatctaa gaggaaaggt atttggcgca acccggtttg gaacactttc
     1921 tgatctcgct tccagattcg ccctgcgcgc ctatgggatt gaacctgaac gtgacatcac
     1981 catggttcaa acgggaggac cggcagaaac agtaaccgcc atggcggcag gcaaggtcca
     2041 cgcggcggca atcactcctc ccgcgacctt gcaggcccgc aaggtcaagc ttaaagagct
     2101 tttggatatg tccaagctgg atgcagaata tcacatcaac ggagtggtga cgacgcggcg
     2161 atatctaaaa accaacgaag atatcgtgag acgtttcctg cgggcataca tcgaaggcgc
     2221 ggcgcgagcg cagaaggata agaactttgc ccttaaggca atgagtaaat attttagaac
     2281 cgacgaccgg gaaatcctcg aggagagtta taatgccgtg atcaaatcca actttgccat
     2341 tccgccctat ccatccatca gcggcattgc cagcttgctg cagggactag agaaacaaaa
     2401 ccccaaggcc aaaggtgcca agcccgaaga tttttcagat agccgcctgg tacgagagct
     2461 cgatcaaagc ggctttataa aatcggttct gccagagcgc tgacaagagc ggctttgatc
     2521 agctccggtg ttccgatcac cgctttcacg ctacccgcga gtgccttgga gtaacttcgc
     2581 agcataggca atcctgcgcg accggtccga ctgcgccact ctgatgtctt cgcacagttc
     2641 caagagctgt gcattgaaat ccggttggat atattcccct cgagcttcct ggaccattgt
     2701 tgactcccgc acccggaatt ctgatgcaaa agtgattggc tgggttctgg cgctcccatc
     2761 cgccttgcgg cgccagaaga taaagccaag aagtaaagcc agatgggcgc gcagccgatt
     2821 tggacaatcg cgtcgtaact ccgtatgccc gccgcccgaa tcgatgctgg gagttgtagt
     2881 tgcgtgcgtt cggctgttac actgtcagtt tagctttccg gggggtcttg tatcttactg
     2941 cgggttttcg acattggctc cgagccgtgt ctcaaggatc aaatcatggc ggctgtatac
     3001 cagaaatcct cgccaatgag acgataacat ccgatcaatc aactcctcac ggcacaaacc
     3061 cgcgcacaaa ggtcattagc tttttagagc ggccggtaat gcgtaatcaa ttgtgaagaa
     3121 ctcattaaaa tccttaacct cagcagtatc ggggtgtgtc aaccgggcct ttcgagagcg
     3181 cggcgaacag tattatctcg acggtgccgt caacattatt gaaggaagcg cctggcgggt
     3241 tgcggctacg gtgcaaggaa cccgacgcta cgaagtcatc atcgttcggg cgcgggactt
     3301 ttttcaggtc tcgtgcagct gccctcttta tgaccgcgag ctggtcactt gcaaacacat
     3361 atgggcggtg tttctggcag ccgagcaaag ccgttttctc gacgggttag gtgattctgc
     3421 tccaggggaa atcagagaag agataccggg attgtttaat tatcatcaag ctgaggggag
     3481 cccaacctcg cgtttacctg aggtaagttg gaaacaacaa ctccagcggc ttagcgggtc
     3541 tatggcggca caggatcgcc gttttaatct ggatgccggc ccagagcgct acttcctcta
     3601 tgtcatcgat atgaacggtt gcgttgacag cgaacgacta acgattcacg tggtgcagcg
     3661 cacccgaaaa gctgatggca cttggggtaa gctcaaaacc caaaaaatcc gcttcaacga
     3721 aatcgctacc ctgcccgatc cggccgatag gcggatcctc gcgctgttgg ctggagcgcg
     3781 cgaggaaacc aattacggtt attcgccgta ctacgattca gttcagtcga ccttcagcct
     3841 agccgaacct tcatgggatc tagtgttgcc actgatatgc gccacagaac gatgcgtact
     3901 cggtctctca accgacccag atcagcagtc actggtatgg ggcgaaggcg agcactggga
     3961 attcggcatg gaaatgacgc tcgatgaatc cgcaggaacg tattctgtgc agggtctgtt
     4021 gagacgtgga gacgtagaaa tggacctcca acgtccgcag ctcatcattg ctggcgggct
     4081 gatagtttat gacggtcgca tcgctcgtct gcgcgatttt ggtgcgttta gctggatcgc
     4141 gcttctgcga gaccacggac cgatatctgt cccgaaaaca gaaggcgatg aattcgtcaa
     4201 tgagatcctc cgtatgccgc gacgcccacg gcttaaattg ccgcacgagt ttagcttcga
     4261 ggaagtcact tgccgtccga acccggctct ggcgataagc tctaagactg agcgcaaggc
     4321 ggcaaacgct cgtctgctcg ccaatctttc ttttgaatac gacggcaccc tggtgccgca
     4381 cagatacccg ggacaagcca tacatcaaca gaatcgtcgc cgcttgatca atcgcgactt
     4441 tgccgtggaa gatgcggcgg ttactcgctt aaaagagctt ggcttccgcc ggccgcattg
     4501 gcccggttcc gggagcgaat ttgaattctc accggatcgt cttccgaatg tagtgcgcgc
     4561 gctcagcggc gagggctggc gagtcgaagc cgaggggaaa atttatcgct ccgccagcgc
     4621 cctgcgtctc aatgttaagt cgggagttga ttggtttgaa cttgagggcg gcgcccaatt
     4681 tgatcaagtc caaatggcgc tgcccaaact gctgcgtgct ataaagcgcg gcgaagagac
     4741 cgtccggctc gatgacggca ccctcggcat cttgccggaa gattggctgg aaaaataccg
     4801 gctgctagcg ggcgtgggca gcatgcatgg cgatcagctg aggtttagcc gaaagcaagt
     4861 cggcttgatc gatgcgctca ttgccagtga agagaatgtg tctgtcgatg gcgggttcga
     4921 gcgcgtgcgg caagaactct actcattcgc cggggtcgag gctgccgatc cgacacctga
     4981 cttcagtggg catttgcgcg actatcaacg cgaggggttg ggctggctcg catttctgca
     5041 gcgcttcggg ttgggtggtt gcctggccga cgacatgggg ttgggcaaaa cagttcaggt
     5101 gctggcgctc
//
LOCUS       DASUTG010000062         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1788822, whole genome shotgun sequence.
ACCESSION   DASUTG010000062 DASUTG010000000
VERSION     DASUTG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788822"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VF089_01335"
     CDS             complement(<1..1016)
                     /locus_tag="VF089_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6172627.1"
                     /translation="MLRELRIKNFAVIDEVVLQLGQGLNIITGETGAGKSIILSALGL
                     LSGERGSSEIIRHKAEEATVEALFETVPPVLSAQLSEAGFEVDGELIIKRILNRSGKN
                     RIYLNGSLCPLGLLSQIGASLVHIYGQHEHHTLLQSETHLNLLDAFADLDSRTSSMKQ
                     RFEGLSNAWKLLTETRDSLERQRREKALLEAQAEEIAQSGLKPGEEEELRSSRNILAH
                     SERLYQGCREGEEILYEGESALVGRLGKYVVKARELAAIDQELSATVELLESSLAQLQ
                     EAALLLGRYAERVHFDPRALEQLDDRLAEIQRLKRKYNGDIEEILRLQTGIQVTLDNL
                     QQGEA"
     gene            complement(1024..1875)
                     /locus_tag="VF089_01340"
     CDS             complement(1024..1875)
                     /locus_tag="VF089_01340"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX6172628.1"
                     /translation="MIRAVGLVAKYHEPKATEMARWLVPWLKKRGLKVYVENGISRSG
                     GISSSKKEMAAKADLIISLGGDGTLLNIAPLVERPDVPILGVNLGGLGFITEVAADEL
                     ESVLSQTLEGEYQTEQRMTLEVQVHSKNGKAHRFRVLNDAVITKGARSRIINLETYVD
                     GEYLCNYRADGLIISTPTGSTAYSLAAGGPILAPALSAIILTPLCPHTLTNRPIVVPG
                     IAGIRVTLRSFGDTVFLSPDGQQGVRLENGDKVEARDYGLPVSLIKLPSRSYYEILRE
                     KLKWGER"
     gene            complement(1981..>3409)
                     /locus_tag="VF089_01345"
     CDS             complement(1981..>3409)
                     /locus_tag="VF089_01345"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172629.1"
                     /translation="MVMTYAEELVAGDPRHSSMVKIKSGVKKDGTLTARETKVIFNSG
                     AYASFKPGDPPNLGGATFTNGVYHIPHYSIRSYGVYTNSVPCGYYRAPGQPQAVFAAE
                     SHMDLIAQELRMDPLELRLKNLLRDGEKLAGGRAVDKVRVRETLQAAAQKAGWGKKNA
                     NNTGLGIGVSFRHVGGSGTASAELSLSADGKLKILTAVPDIGTGTHTLLRQIAAEVLT
                     VPPELILTEIGDTESFASDAAPGGSKITNMSGHVVLQAAQQLRDRLTPVAASLLGCAV
                     SDVVLQNGRFGSAREKKSSIEFRDVAQEATKANGELRVRETFEVKGRSPVPAFVVQIA
                     QVQVDVETGQLKVKRIVTAHDVGTVINPVAHQGQIEGGLVQGLGYATLEEVITENGKV
                     LTTNLGDYRIPCPRDLPTLETVLIEDPTGPGPFAAKQIGENGIIATAPAIANAVAAAV
                     GVRLFDTPLTAEKIYLALKSSSPAG"
BASE COUNT          728 a         1003 c          882 g          796 t
ORIGIN      
        1 gcttcgcctt gctgcagatt atccaaggtg acctgaatcc cggtttgtag acgcagaatc
       61 tcttcgatat cgccgttgta cttacgtttg agacgctgaa tctcggctag acggtcatcc
      121 agttgctcca gcgcgcgcgg atcaaaatga actcgctccg cataacgacc cagtagtaat
      181 gccgcctctt gcagttgcgc cagcgaagat tcaagtagtt ccaccgtcgc gctcagttcc
      241 tgatcgatgg cagccaactc ccgcgcctta acgacgtatt ttcccaagcg cccaaccagc
      301 gcggactccc cttcgtaaag aatctcctcc ccttcacgac aaccctggta gagtctttcc
      361 gaatgcgcca gaatatttct gctcgagcgc agctcctctt cttcgccggg cttcaagccg
      421 gattgagcaa tctcttcagc ctgagcttct agaagagctt tctcccggcg ctgtcgttcc
      481 aaagaatcac gggtttcggt cagcaatttc caggcgttgg ataggccttc gaagcgctgc
      541 ttcatagagc ttgtcctgct atcgagatca gcgaaagcgt cgagcaggtt taagtgggtt
      601 tccgactgca gcaaggtatg gtgctcgtgc tggccataga tatgcacgag agaagcacct
      661 atctgcgaga gcaagcctaa tggacagaga ctgccgttta aataaatccg gtttttgccc
      721 gaacgattaa gaatgcgctt gatgatcagt tcgccgtcga cctcgaaacc ggcttcgctt
      781 aattgcgcac tgagtactgg cggcacggtt tcgaataaag cttcgactgt ggcctcctct
      841 gccttgtgtc tgatgatctc agaactgccg cgctccccgg agagaagtcc caaggcactg
      901 aggataatcg atttgccggc gccggtttcg ccggtaatga tgttgagtcc ctggccaagt
      961 tgcagaacga cttcgtcgat gacggcgaaa ttcttaatcc ggagttcacg caacatgacc
     1021 cgcttaacgt tccccccatt tcagtttctc gcgcaagatt tcatagtagc tgcggctcgg
     1081 cagctttatc aacgataccg gcagaccgta gtcccgtgcc tcgactttgt cgccgttctc
     1141 gagccgaaca ccctgctgtc cgtcgggact caagaaaacg gtatcgccaa acgagcgaag
     1201 cgtcacccgt attcccgcga ttccaggcac cacgataggt cggttcgtca gtgtgtgagg
     1261 acacagggga gtcaggatga tggcgctgag cgccggcgcc agaatcggcc cgccggccgc
     1321 gagcgaataa gcagtagaac cggtgggcgt tgaaataatc aacccgtcgg ctcgatagtt
     1381 acataagtat tcgccgtcga cataggtttc caaattgatg atgcggctgc gcgctccttt
     1441 ggtgatcacc gcgtcgttta acacgcgaaa gcgatgagct ttgccgtttt tactgtggac
     1501 ctgaacttcc aaagtcatgc gctgctctgt ttgatattca ccctccaacg tctgcgaaag
     1561 aaccgattct aactcatcag ccgcaacttc ggtgatgaat cccagacccc ctagattaac
     1621 tccgagaatc ggcacatccg gtctttcgac tagtggcgcg atattgagca aggtcccatc
     1681 gccgcccaaa gaaataatca aatcagcttt ggccgccatc tcctttttgc tggaagagat
     1741 accgccgctg cgggaaatgc cattttccac gtaaaccttc agacctcgtt tctttaacca
     1801 ggggaccagc caacgggcca tctctgtggc tttgggctcg tgatattttg ccaccaatcc
     1861 tacggcacga atcatcgaag ttgcttctcc cgagtactta tttcacttgt gcgagacatc
     1921 tatcgatcag ctaacgggga ttatcgcgga tgctttaaca tcgacccgct ctagcagcgc
     1981 ctatccggca ggcgaggacg atttcaacgc caggtaaatt ttctctgcag tcagaggagt
     2041 atcgaacagc ctcacgccca ccgcagccgc cacagcattg gcaattgcgg gagcggtggc
     2101 gataatcccg ttctcgccga tctgcttggc ggcaaatgga cccggccccg tgggatcctc
     2161 gatgagcacc gtttctaaag tcggcaaatc ccgcgggcac ggaatgcggt aatcgcctaa
     2221 attcgtcgtg agaactttgc cgttctcggt gatcacctcc tcgagagtgg cgtaacccaa
     2281 tccctgaacc aaaccgcctt cgatctggcc ctgatgcgcc acggggttaa tcaccgtgcc
     2341 cacgtcatgt gccgtaacga tccgttttac cttcagctgt ccagtttcga cgtcgacttg
     2401 gacttgggcg atttgcacca caaaggcggg caccggcgat cggcccttta cctcgaaggt
     2461 ttcacgcacg cgcagctcgc catttgcctt agtcgcctct tgcgccacgt cgcgaaactc
     2521 tattgacgat ttcttctccc gcgcgctgcc gaaacggccg ttctgcagaa ctacgtcact
     2581 gacggcgcaa ccgagaagcg atgctgcaac gggagtcaat cggtcgcgca gctgttgagc
     2641 cgcctgcaac accacgtggc cgctcatatt ggtgatcttg ctgccgcccg gcgctgcgtc
     2701 gcttgcaaag ctttcggtat cgcctatttc agtgagaatc agctcgggcg gaaccgttaa
     2761 aacttccgca gcgatttgcc gcagcaaggt atgcgttcca gtgccgatat ccggcacagc
     2821 ggttaagatc ttgagtttac cgtcagcaga tagagaaagc tccgcgcttg ctgtaccgga
     2881 gccaccaaca tgccggaacg agacgccgat cccgagaccg gtattgttgg cgttcttttt
     2941 tccccaaccg gccttctgcg ccgccgcctg caacgtttcg cgcacccgta ctttatccac
     3001 cgcccggccg ccggctaact tctcaccatc gcgcaggaga ttttttagcc gcaattcgag
     3061 cggatccata cgcaactctt gggcgatcag atccatatga gactcggcgg caaacaccgc
     3121 ctggggttgc ccgggagcgc gataatagcc gcacggtaca gagttggtgt aaacgccata
     3181 ggaccggatt gagtaatgtg ggatatggta gacaccattg gtaaaggttg ccccgccgag
     3241 attcggcgga tcgccgggct taaacgatgc atatgcgccg ctgttgaaga ttactttggt
     3301 ttcacgcgct gtgagagtac cgtctttctt gaccccgctt ttgatcttga ccatcgagga
     3361 atggcgtgga tcgccggcaa ctagctcttc cgcataggtc atgaccatc
//
LOCUS       DASUTG010000063         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_10086, whole genome shotgun sequence.
ACCESSION   DASUTG010000063 DASUTG010000000
VERSION     DASUTG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10086"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..920
                     /locus_tag="VF089_01350"
     CDS             69..920
                     /locus_tag="VF089_01350"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172630.1"
                     /translation="MLAQSFGSWSLIIILASPGWPAEVDDPVSPVDPRNGCPQCRNDS
                     TQIAQLHKNADKLYAQFKPKEAANELLKILRMDPQNFEALIKLSRAYIDLGDMIPEST
                     SDWKERRLKDYRTAEDFARRAIKANPNSTWGYFYLGASLGLTAVVSPIARQVELADEI
                     RVALEKSLALDANNGFAYHAYGVWHRKISEIGKMSRVFASVLYGRSLPQGTLEKSVEY
                     LKQAIALNPNVIVSRLELANTYMAMEDFQAARTMLSSIRNLPIQFSDDAKHKQKAEEL
                     LEEVASR"
     gene            992..1909
                     /locus_tag="VF089_01355"
     CDS             992..1909
                     /locus_tag="VF089_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6172631.1"
                     /translation="MLAPHRSRFCITLFVILCLIGCGDSGRSTKVLRVGFVPAEDAQQ
                     VMQNAQPIVDILQKELGMEIQPFVAADYTGVVEALRVNKLDVAFLTPASYVLAKNEAN
                     VKVVLKSERKGIASYYAAIITRADSGIQRLEDLRGKTFAFGDALSTTGNIFPRKMFKE
                     RGIDPVRDFKQILYSGGHDATVLAVLNGKVDAGATYANSPDSDDTAWMRYLKDPADVK
                     KIRAIAFSEPIPADNLVISGNLDEAIAKRVEEIFLQLSRDPKGKQMMRDLYQIDGFVP
                     ATDQDYDSVRQAFDIAGIPLKTSLQRKQP"
     gene            1906..2685
                     /gene="phnC"
                     /locus_tag="VF089_01360"
     CDS             1906..2685
                     /gene="phnC"
                     /locus_tag="VF089_01360"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX6172632.1"
                     /translation="MIILQIEDLHKSYDSNVPVLCGINLSVHRGEFVGIIGLSGSGKS
                     TLLRCINRLVEPSSGHIRLPRGLITGNGAGTLVDVVGLKQTELRRLRRKIGMVFQQFN
                     IIKRLSVMENVLSGGLGYQSPLRSTLRTFTRDEKRKALTNLKRVGLLDHAYRRADELS
                     GGEQQRVAIARTLMQEPALILADEPVSSLDPKLSRVVLDILNRVCQEDGITALVSLHT
                     LELTREYAARVVGLKNGQICFDGTPGELTRPVVEAIYHDEL"
BASE COUNT          695 a          784 c          783 g          639 t
ORIGIN      
        1 tcacggaagc tgaggttgtc tacgtcgcca tagacgaggc cgggaagccc ttatccatag
       61 gatagaacat gctggcgcag agcttcggca gttggagtct gatcatcatt ctagcatcgc
      121 cgggatggcc tgcggaagtt gatgacccgg tttcgcctgt ggatcccaga aacgggtgcc
      181 ctcagtgtcg caatgacagt acccagattg cgcaactgca taagaatgcc gacaagctct
      241 atgctcaatt caaaccgaag gaagccgcca atgagttgct gaaaattctg cgcatggatc
      301 ctcagaactt cgaagctctc atcaaactgt ctcgggcata tatcgacctt ggagacatga
      361 tcccggaatc aacctcggat tggaaggaaa gacgtttaaa ggattatcga acggccgaag
      421 atttcgcccg ccgagccatc aaagccaatc caaactcgac ctggggctat ttctacctag
      481 gcgcctcgtt gggcctgacg gcggtcgttt ccccgattgc aagacaggta gaattggcgg
      541 atgaaatccg cgtggcgctg gagaaatcgc ttgcattgga tgcgaacaat gggtttgctt
      601 atcatgctta tggcgtttgg catcgaaaga tctctgaaat cggaaaaatg agccgggtgt
      661 tcgcttcggt tctctatggc cgctctctgc cgcaaggcac cctggaaaag tcagtggagt
      721 atttaaaaca agccattgcg ctcaatccga acgtcatcgt aagccggctc gaattggcaa
      781 atacttacat ggccatggaa gatttccagg ctgcccgcac gatgctctcc tcgatccgga
      841 atctaccgat tcaattctcc gacgatgcca agcacaagca aaaagccgaa gagttgttgg
      901 aagaagttgc aagccgctaa aaaccgctct ccctggaccg atagtgagaa cgttgcgccg
      961 ctagcggtga atctgtcacg atcatgcaat aatgctcgca ccacacagat cacgattttg
     1021 tatcacctta ttcgtaatcc tctgcctgat tggctgcggt gattccggca gatcgaccaa
     1081 ggtgcttcgc gtcggcttcg tgccggcgga agatgcccaa caagtgatgc aaaacgctca
     1141 gccgatcgtt gacatactgc aaaaagagct gggtatggag atacagccgt ttgtcgcggc
     1201 tgattatacc ggcgtggtcg aagcgctccg ggttaacaaa ctcgatgtcg cttttttgac
     1261 ccccgcctcc tatgtactgg cgaagaatga agctaacgtg aaggtcgttc ttaagtcaga
     1321 gcgcaaggga atcgcctcat actatgcggc aatcatcacc cgagcagaca gcggcatcca
     1381 aaggctcgaa gacctgcgcg gcaaaacttt cgctttcggc gacgcgctat caaccaccgg
     1441 caacatattt cctcgcaaga tgttcaagga acgcggcatc gatccggttc gcgacttcaa
     1501 acaaatactt tattcaggag ggcatgacgc gacggtgctc gccgtgctca acggcaaagt
     1561 cgacgccggg gcgacctatg caaattcccc cgacagcgat gataccgcat ggatgcgcta
     1621 cctgaaggat cccgcggacg tgaagaagat ccgcgcgatc gcattctccg agcctattcc
     1681 ggcagataat ttagtgatca gcggcaatct cgatgaagcc attgccaaaa gagtggaaga
     1741 aatttttttg cagctgagtc gcgatcctaa aggcaagcag atgatgcgtg acctctacca
     1801 gatcgacggt ttcgttcccg ccactgacca agactatgat tccgtgcgtc aggctttcga
     1861 cattgccggt atccccctga aaacaagctt gcaaagaaaa cagccgtgat cattctgcaa
     1921 atcgaggatc tccataaatc ctacgatagc aatgttccgg ttctctgcgg catcaattta
     1981 tccgttcatc gcggcgaatt cgtcggcatc atcggactta gcggatcggg aaaatcgacc
     2041 ttgctgcgct gtatcaatcg tttggtcgag cccagctccg gccatatccg cttgcccagg
     2101 ggactgatta caggaaacgg cgccggcacc ttggtcgatg tcgtgggatt gaagcagacc
     2161 gaattacgac ggctcagacg taagatcggc atggttttcc aacagttcaa tatcatcaag
     2221 cgcttgtcgg tcatggaaaa tgttctttcc ggagggttgg gctatcaatc gccgctgcgc
     2281 agcacgctgc gaacttttac ccgagatgaa aagcgcaagg ctttgaccaa tctcaagcgc
     2341 gttgggctgc tggatcatgc ctaccgccgc gccgacgaac tcagcggcgg tgaacagcag
     2401 cgggtggcga tcgctcgaac tttgatgcaa gagccggcgc ttatcctggc cgacgaacct
     2461 gtttcgagcc tggatcccaa gttgtcccgc gtcgtcctcg atattctaaa tcgagtctgt
     2521 caggaagacg gcatcacggc actcgtgagc ttgcatacgt tagagttgac ccgagaatat
     2581 gccgcccggg ttgtcgggct caagaacgga cagatctgtt tcgatggaac ccctggcgag
     2641 ctaacccggc cggtggtcga ggccatctat cacgacgagc tatgaaaccc ggatgcttgc
     2701 gtgtgctcgg cagtggggct gttgaacgct tgacggtcgt ccgtgctggc ggccagctca
     2761 acctatcaac cccaggtggc gccaccaggg gatcgcgact agggccgcca atacgtaggc
     2821 gacggcgtac accggattga tgcggtacat cataggatag ctaaatcctt tttccccggt
     2881 cgtcgcgctt gccatggtat g
//
LOCUS       DASUTG010000064         8286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_306632, whole genome shotgun sequence.
ACCESSION   DASUTG010000064 DASUTG010000000
VERSION     DASUTG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306632"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="ahcY"
                     /locus_tag="VF089_01365"
     CDS             complement(<1..999)
                     /gene="ahcY"
                     /locus_tag="VF089_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6172633.1"
                     /translation="MKYDIKDIGLAQSGRKRILWAGQDMPVLQRVRERFQKEKPFQGM
                     RFSACLHVTAETANLVQVLKAGGADVLLCASNPLSTQDDVAASLVQHDRIPTYAIKGE
                     DNKSYYSHLRAALDHKPVITMDDGADLVSLLHTDYSHLASGLTASMEETTTGVIRLRA
                     LEKEGALKIPVIAVNDAATKHLFDNRYGTGQSTIDGVIRATDMLIAGKRVVVAGYGWC
                     GKGVAARARGMGAKVIVTEVDPVRALEAAMDGFDAMTMKEAARIGDLFITLTGDMHVI
                     SGDHMKLMKDGAVICNSGHFDIEIDLGALKKLSTKVEKNIRNSVDAYTLPGGKRIFL"
     gene            complement(1038..2216)
                     /gene="metK"
                     /locus_tag="VF089_01370"
     CDS             complement(1038..2216)
                     /gene="metK"
                     /locus_tag="VF089_01370"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX6172634.1"
                     /translation="MAGKDFLFTSESVSEGHPDKLCDQISDAVLDAHLARDPDSRVAC
                     ESLAKTGMIVIAGEITSKAQVSYSEIAREIVRDIGYTDSAMGFDGNTCAILTAVEQQS
                     PDISQGVTEGEGLHKEQGAGDQGMMFGYACDETPEFMPLPIQMAHSLVKYLAKIRKGG
                     EAYFLRPDSKSQVTVEYRGGKPIKVKSVVISSQHSPEVEYSKLRETIIDGVIKAVIPP
                     EYLSKDTVFHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSAAYMARYVAKNIVAAELAQRCEVQLAYCIGVAQPVSVLVDTFGTGIISETTIAKIV
                     QEVFNLTPKGIIQTLDLKRPIYRSTASYGHFGRTPEDGHFTWEKTDRVEDLKKAAACA
                     "
     gene            complement(2288..2563)
                     /locus_tag="VF089_01375"
     CDS             complement(2288..2563)
                     /locus_tag="VF089_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX6172635.1"
                     /translation="MVKVVKKMEIKNKLGVHARAAALLVQTVNRFSAQVTISKDGQTA
                     DGRSIMGVLMLAATQGSTIEVEAAGQDAEQAVKAIEKLVDKRFNENE"
     gene            complement(2567..3433)
                     /gene="rapZ"
                     /locus_tag="VF089_01380"
     CDS             complement(2567..3433)
                     /gene="rapZ"
                     /locus_tag="VF089_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6172636.1"
                     /translation="MTDDLQVIIVTGLSGSGKSVAIRALEDNGFFCIDNLPAPLIPKF
                     IDLCIGYNEGITRIALGVDLRAGQFLQALPEVLADMRSAGHHVQVLFFDASDEVLLRR
                     FSETRRPHPLAGEEPIQEGISRERRALEAMRELADKVIDTSNLNVHELKREMEERFCQ
                     APYSRSMNVFLTSFGYKYGIPHDTDMILDVRFLPNPYFVSELRAHDGLDREVEEYVLK
                     NEETRIFLDRLYSLLEFTLPLYEREGKSSLTVALGCTGGRHRSVVLVEELHKRLGDGK
                     FRMHVKHRDIEK"
     gene            complement(3440..3901)
                     /locus_tag="VF089_01385"
     CDS             complement(3440..3901)
                     /locus_tag="VF089_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX6172637.1"
                     /translation="MKITDFLTLDRVIADLSGKGKSEVLEEMANQLADRPAGLDPAQV
                     LRVLQDREKISTTAIGEGVAIPHGKLPTVEKIWGLFARSSQGVDFASLDGEPTYLFFT
                     LIAPQDAAADHLKALARISRLLKDPDLRQRLLAAGNSQELYNIIATEDQKF"
     gene            complement(3898..4218)
                     /gene="raiA"
                     /locus_tag="VF089_01390"
     CDS             complement(3898..4218)
                     /gene="raiA"
                     /locus_tag="VF089_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX6172638.1"
                     /translation="MSDVKVSVTFRHTEPTDALKHYAEEKMHRLGKYFSPSLDAHVVL
                     SVESKERQTAEVELHAHGAMIHAKEQTEDLYSAIDLVTDNLKRQLRKQKDKTKLEPRR
                     KLPE"
     gene            complement(4243..5712)
                     /gene="rpoN"
                     /locus_tag="VF089_01395"
     CDS             complement(4243..5712)
                     /gene="rpoN"
                     /locus_tag="VF089_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX6172639.1"
                     /translation="MALEIRQVQKLAQQLVMTPQLQQAIKLLQLSRVELEDMVTKELQ
                     ENPALEEGPAEESESGDQSRSENAGEVGLTEPVVNRELSAVDKIGTYDWQEYLDTHSN
                     SMHGSLTAEAGSDDGDAPPSWENSLTKKTSLEDHLLWQLRLSKITERESMIGQYMIQN
                     LSENGYLALTLEEVCAATQTDPEEAERVLKRIQFFDPVGVAARNLRECLLVQLQNLEL
                     GDSLAARIVSDYLGFLESKRYEKLARDLGTTIDEIADAAHLITSLEPKPSRGFEQDDV
                     RTVLPDVFVEKIAGEYVIYLNDDGVPRLRVSPLYRRMVGQEGVAEEQARQYLQEKVRA
                     ATWLIKSIQQRQQTLYRVTQSIFKFQLDFLDHGVSQLRPMVLRDVAEDIHMHESTVSR
                     ATANKYVHTPQGLFELKFFFQSGLRGGNGEDVASESVKEKIRGIIAGEDPRKPFSDQH
                     IAALLSDCSIDIARRTVAKYREAMGILPSSKRRQPFFRK"
     gene            complement(5714..6436)
                     /gene="lptB"
                     /locus_tag="VF089_01400"
     CDS             complement(5714..6436)
                     /gene="lptB"
                     /locus_tag="VF089_01400"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX6172640.1"
                     /translation="MSVLKAEAVAKSYNGRRVVNGVNLEVHGGEVVGLLGPNGAGKTT
                     TFHMMVGLVHPNEGKVLLNDTDLTAAPIYQRARAGISYLPQESSVFRRLTVEDNLQAI
                     LQTLDLNDVERQERCDTLLNMLGVAHLAKNKAFTLSGGERRRVEIARALVLSPQFVLL
                     DEPFTGVDPIAVAEIQKIIRELITSDIGILITDHNVRETLGICDRAYILNEGAILEQG
                     TPEDISESPKARKFYLGEGFKF"
     gene            complement(6433..7008)
                     /gene="lptA"
                     /locus_tag="VF089_01405"
     CDS             complement(6433..7008)
                     /gene="lptA"
                     /locus_tag="VF089_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX6172641.1"
                     /translation="MKFPSLTFGLAVLLLAGFIVGLARGASRDDAKNKSDDSVFEFNK
                     KEPIYITSDWMEVDQKKNTITYKGRVVTIQSEMTMRSETLIAVYDPEMKQIRQIIAEG
                     KVNAVQGKRMATGDKAIFDEKAKTVTLTGNPVMRQGNSQVTGSRIIYYIDQDRAVAEG
                     KDSVRVQVTIFPDELQKERDGKSEAGSSTAK"
     gene            complement(7020..7631)
                     /gene="lptC"
                     /locus_tag="VF089_01410"
     CDS             complement(7020..7631)
                     /gene="lptC"
                     /locus_tag="VF089_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX6172642.1"
                     /translation="MRKRRFLLLIAIVASLAGVGYKVTAEIWANKLRDIRENPIQLLD
                     QLPESALSIKDFRRAKVENGRTVWELLGEEASYDKDQRQAVIQKPRFLYYDKKGDVAE
                     ASAEIAHIYLNDKELEKMQLEGGIEISYNGYLLKSQEAIYLPAKEQIVMPQRTTLISE
                     GLELQGSSMEVDLGDSKVRLLRDVKTKLEPEKLAKQKAKSGES"
     gene            complement(7720..8223)
                     /locus_tag="VF089_01415"
     CDS             complement(7720..8223)
                     /locus_tag="VF089_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX6172643.1"
                     /translation="MLLLDVDGVLTDGRIILDDRGVETKQFDVRDGQGIVLLLRAGIE
                     VGLITARNSKAVRYRAKELGLRIVHQGVQSKASTYGAIRRQSGLAETQIAYIGDDIGD
                     LEVLHRVGLAVTVNDGSEELFRSVDYITQKPGGRGAVREVAELLLKAQNKWIHPRKNS
                     NNIDFLH"
BASE COUNT         1812 a         2325 c         2013 g         2136 t
ORIGIN      
        1 gagaaaaatc ctcttgccgc cggggagcgt ataggcgtcg acagagttgc ggatgttttt
       61 ctctactttg gtcgacagct ttttcagcgc accgagatcg atctcgatat cgaagtgacc
      121 cgaattacag atcaccgcgc cgtctttcat gagcttcata tgatccccgg agatcacatg
      181 catgtcgccg gtcagtgtga tgaaaaggtc gccaatccgt gccgcttctt tcatggtcat
      241 cgcgtcgaaa ccatccatgg ctgcctcgag agcgcgaacc ggatcgactt ctgtgacgat
      301 gaccttggcg cccattcccc gcgcccttgc cgccacccct ttgccgcacc aaccgtagcc
      361 tgcaacgaca acgcgcttgc cggcgatcaa catgtcggta gctcgaatga cgccgtcgat
      421 ggtggattgc ccggtgccat agcggttatc gaagagatgc ttggtcgcgg catcgttcac
      481 cgcgatgacc gggatcttta gcgcgccctc tttttccaac gcgcgcaagc gaatcacacc
      541 tgtcgtcgtt tcttccatac tcgcggttaa accggatgcc aaatgggaat aatccgtatg
      601 tagcaaagag actagatcag cgccgtcatc catcgtgatg accggcttgt ggtccagcgc
      661 ggctctaaga tggctataat agctcttgtt gtcctcgccc tttatggcat aggtaggaat
      721 tcgatcgtgt tgcactaacg aagcggcgac gtcgtcttgc gtcgacaacg gatttgaggc
      781 gcatagcagc acatcggcgc cgccggcttt cagcacctgg acgagattcg cggtctccgc
      841 cgtgacgtga agacaggccg aaaaacgcat tccttgaaac ggcttttctt tttgaaatcg
      901 ctcccgaacg cgttgcagaa caggcatatc ctgccctgcc cataggatcc gctttctgcc
      961 gctttgagct aaaccgatat ccttgatgtc gtatttcatc gatgccatgc tgcgaacttc
     1021 ctcccgccgc accgcgctta tgcgcatgcc gccgctttct tgagatcttc aactcgatcg
     1081 gttttttccc acgtgaagtg gccatcttcc ggcgtgcgtc caaaatgccc gtaggatgct
     1141 gtggagcgat agataggcct tttcaaatcg agtgtttgaa tgattccctt gggtgtcaaa
     1201 ttaaaaactt cttgaacgat cttggcgatg gtcgtctctg aaatgatgcc ggtgccaaaa
     1261 gtatcgacga gcacggaaac aggttgggca actccgatgc agtaggcgag ttgaacctcg
     1321 cagcgctgag ccaactctgc tgcgacgatg tttttggcaa catagcgcgc catgtatgcg
     1381 gcgctgcgat cgactttgga cggatccttc ccagagaatg cgccaccgcc atgccggccc
     1441 attccaccat acgtatcaac aataattttc cggccggtaa gaccacagtc tccctgaggc
     1501 ccgccaacaa caaaccgccc ggttgggttg acatgaaata cggtgtcctt gctcaggtac
     1561 tccggcggga tcaccgcttt tatgacgcca tcgatgatcg tttctctgag cttcgagtat
     1621 tccacttcag ggctatgctg gctggagatg acgacgctct taaccttgat cggcttgccc
     1681 ccgcgatatt ccacggtaac ctgagacttg ctgtccggcc gcaagaagta ggcttcgccg
     1741 cctttgcgga tctttgccag atacttaacc agactgtgag ccatctgaat cggtagcggc
     1801 atgaactcag gagtctcatc gcacgcatag ccgaacatca ttccctgatc gccggcgccc
     1861 tgctctttat gcagtccctc cccttcagtg acaccttgag aaatgtcggg agactgctgc
     1921 tcgaccgcgg taagaatagc gcaagtattg ccatcaaacc ccatggccga atcagtgtag
     1981 ccgatatcac gcactatctc tctagcaatc tcggaatagc tcacctgggc tttgctggtg
     2041 atttctccgg cgatcacaat catgccggtc ttcgccagac tctcacaggc cacacggctg
     2101 tccggatcgc gcgctagatg cgcatccaac acggcatcgg aaatctgatc gcacaacttg
     2161 tcgggatgac cttcagagac cgattccgat gtgaatagaa aatctttgcc agccatggtg
     2221 aacctccaga cacgcttgga actgattaat ccctgtcata tagccaataa gaggggggga
     2281 ttcaacctta ctcgttctcg ttgaatcttt tgtcgactag cttttcgatg gctttgaccg
     2341 cctgctcagc gtcctgacca gccgcttcca cttcgatagt gcttccctgg gtcgcggcca
     2401 acataagcac gcccatgatg ctacggccat cagcggtttg accatccttt gagatagtga
     2461 cttgcgctga aaaccggttt acggtttgaa ccagcagcgc tgcggcccgc gcatgaaccc
     2521 caagtttgtt tttgatttcc atttttttaa cgaccttgac catggatcat ttttcgatgt
     2581 cacgatgctt gacgtgcatg cggaatttgc cgtcacccaa tctcttatgt agctcttcca
     2641 ccaggacgac cgaacggtgg cgcccaccgg tgcagccaag tgcgactgtc aaactgctct
     2701 tgccttcgcg ctcgtacagc ggcaaggtaa actccagcaa cgaatacagc cgatcgagaa
     2761 aaatccgggt ttcttcattc ttcaggacgt attcttccac ctcgcgatcc aaaccgtcat
     2821 gagcgcgcaa ttcgctgaca aagtaaggat tgggcaaaaa ccgaacgtcc aggatcatat
     2881 cggtgtcatg tggaatgccg tacttgtaac caaacgacgt cagaaacacg ttcatgctgc
     2941 gggaatatgg cgcctggcag aaccgttctt ccatttcccg tttgagctca tgcacattca
     3001 aattgctagt gtcgatcacc ttgtcagcca gttctcgcat tgcctcaagc gccctgcgct
     3061 cacgcgaaat cccttcctgg ataggttctt cgcctgcgag cggatgaggt cgccgggtct
     3121 cactaaaccg cctcaacaag acttcatccg aagcgtcgaa aaagagcacc tggacgtggt
     3181 gcccggcact acgcatatcg gccagaacct ccggcaacgc ttgcagaaat tgcccggctc
     3241 tcaggtctac gccaagagcg atccgggtga ttccttcgtt gtaaccgata cagaggtcga
     3301 taaattttgg gatgagcggc gcaggtaaat tgtcgatgca gaaaaaaccg ttatcttcca
     3361 gcgcccggat cgcgacactt ttcccagacc ccgaaagacc tgtgacgatg atgacttgta
     3421 gatcatccgt cataccagat caaaacttct gatcttcggt ggcgatgatg ttgtaaagtt
     3481 cctgactgtt tccggcagcc aatagccgct gccgcaaatc gggatctttc agcaaacgcg
     3541 agattcttgc cagcgccttt aaatgatccg ccgctgcgtc ttgaggggcg atcagagtga
     3601 aaaacagata ggtgggttcg ccgtcgagcg atgcaaaatc aaccccctgc gagctgcgcg
     3661 caaataagcc ccatattttt tcaacggtcg gcaacttgcc gtgcgggatt gccacgcctt
     3721 cgccaatcgc tgtggtgcta atcttttccc gatcctgcaa aacgcgcaac acctgagcag
     3781 gatcgaggcc cgctggtcta tcggcgagtt gattggccat ctcctcgagg acttccgatt
     3841 tcccttttcc gctgaggtct gcaatcaccc gatcgagggt taaaaaatcg gtaatcttca
     3901 ctctggcagc tttcttctgg gctcgagctt tgtcttatcc ttttgttttc gcaattggcg
     3961 tttcaagttg tcggttacga gatcgatggc tgaatagaga tcttcagttt gttctttagc
     4021 atgtatcatg gcgccgtggg cgtgcagttc cacttcggct gtctgccgtt ctttagactc
     4081 aactgaaaga accacatgag cgtctaagga cggagaaaaa tatttgccta gccgatgcat
     4141 tttctcttct gcgtaatgct tcaatgcgtc tgtaggctcg gtatggcgaa aggtaacgga
     4201 gactttcacg tcgctcatcg tgctgtgttt tcctttgatt tctcatttac ggaagaacgg
     4261 ctggcgccgc ttggaggagg gaagaatacc catcgcttcg cggtacttgg ccacggttcg
     4321 gcgcgctatg tctatggagc aatctgacag cagtgcggct atgtgttgat cgctgaacgg
     4381 tttgcgcgga tcttcgccgg caatgatgcc gcggattttc tctttaacgc tctcgctggc
     4441 gacatcctcc ccgtttccgc ctctcaggcc gctttggaaa aaaaacttaa gctcgaacag
     4501 tccttgtggg gtgtgaacgt atttattggc cgtcgcccgg ctgacggtag attcgtgcat
     4561 atgaatatct tcggcaacgt cacgcaacac catcggcctc agttggctaa caccatggtc
     4621 gagaaaatcc agctggaatt taaaaatact ttgagtcacg cggtaaagag tttgctggcg
     4681 ctgctgaatg cttttgatca gccaagtggc ggcacgcacc ttttcctgga gatactgccg
     4741 tgcctgctct tccgcaacgc cctcttgccc gaccatgcgg cggtaaagcg ggcttacgcg
     4801 caatcgagga actccatcat cattcaggta gatgacatac tcgccggcga tcttctcaac
     4861 aaaaacatcg ggtaagacag tgcgtacatc gtcttgttca aaaccgcgcg aaggtttcgg
     4921 ttcaagggac gtaatgaggt gtgccgcatc ggcgatctca tcgatggtgg tgcccagatc
     4981 gcgcgcaagc ttctcatatc gtttagactc taagaaacct aggtaatcgg aaactatccg
     5041 agctgctagg ctgtcgccga gctcgaggtt ttgcaattgc accaacaaac actcgcgaag
     5101 attgcgcgcg gcgacgccaa cgggatcaaa gaattggatt cgctttaaaa ccctttcggc
     5161 ttcctccgga tcagtttggg tcgcggcgca tacttcttcc agtgtcaacg ccaagtaacc
     5221 attctcagaa aggttttgga tcatgtattg gccaatcatc gactcgcgtt cggtaatctt
     5281 tgagagccgg agctgccaaa gcagatgatc ttcaagggag gtttttttgg ttaaactgtt
     5341 ctcccaggag ggaggtgcgt cgccgtcgtc cgaacccgcc tcagccgtca gggaaccatg
     5401 catggaattg gaatgtgtgt cgaggtattc ctgccagtca taggtgccga ttttgtcaac
     5461 cgccgatagc tcccgattta ccaccggctc ggttaatcca acctccccgg cgttttcgct
     5521 tcgcgattga tcgccactct cgctttcttc agccggcccc tcttcgagcg caggattctc
     5581 ctgtagctcc tttgtgacca tgtcttccag ttctacacgt gacagctgca gcagcttgat
     5641 tgcctgctgc agctgtggcg tcatgaccaa ttgttgggcg agcttttgaa cttgacgaat
     5701 ttccagagcc atactagaac ttaaacccct cccccaagta aaattttctc gccttcgggc
     5761 tctccgaaat gtcttccggc gtcccttgtt cgagaatcgc gccttcattc aagatgtaag
     5821 cgcgatcgca gatccccaac gtctccctca cattgtgatc ggtgatcaag atgccgatgt
     5881 cgcttgtaat taactcgcgg ataattttct ggatttccgc aactgcgatt ggatccacgc
     5941 cggtaaaagg ctcatcgagc aagacgaact gcggcgacag aaccagcgcc cgggcgattt
     6001 cgactctgcg acgctcgccg ccggagagtg tgaacgcttt gtttttggcc aaatgagcaa
     6061 cgccgagcat gttcagtaat gtatcacagc gctcttgacg ctcgacatca ttgagatcca
     6121 gagtttgaag aatcgcctgc agattatctt caacggtcaa ccggcgaaag accgacgact
     6181 cctgtggcag atagctaatg ccggcacgcg ctcgctggta tatcggcgcg gcagtcaaat
     6241 ctgtatcgtt cagcagcact tttccttcgt tcggatgtac taggcctacc atcatgtgaa
     6301 aggtcgtcgt cttacccgct ccattggggc ccaacaggcc gactacctct ccgccatgaa
     6361 cctccaaatt gactccattg acgacgcggc gcccattata gctcttcgcg accgcctccg
     6421 ccttcaagac actcattttg ccgtgctcga gccggcctca gacttaccat cccgttcctt
     6481 ttgcaattcg tccggaaaaa tcgttacttg cactctaact gagtccttgc cttcggccac
     6541 cgcacggtct tgatcgatat agtagatgat gcgcgaacca gtgacctggc tattgccttg
     6601 tcgcatcact ggatttccgg ttaacgtgac ggtctttgcc ttttcatcaa atatggcctt
     6661 atctccggtt gccatccgct taccctgcac cgcgtttact ttaccctctg cgatgatctg
     6721 cctgatttgt ttcatctcgg gatcataaac cgcgatcaag gtctcgctgc gcatggtcat
     6781 ttccgactgg atcgtcacta ccctaccttt gtaagtaatg gtgttcttct tctgatccac
     6841 ttccatccag tccgaagtga tatagattgg ttccttcttg ttaaattcaa agacgctgtc
     6901 atcgcttttg ttcttggcgt cgtcccggga tgcgccgcgc gcaagaccaa cgataaatcc
     6961 ggccagcaat agtactgcaa ggccaaacgt taagctcgga aatttcatta tcctcgcaat
     7021 tagctctcgc cggacttagc tttttgctta gccagctttt ccggctccaa cttagtcttt
     7081 acatcacgaa gcaggcgtac tttgctgtct cccaggtcca cctccatgct cgagccttgc
     7141 aactccaaac cttcactaat caaggtagtt cgctgtggca tgacaatctg ttcctttgcc
     7201 ggcaggtaaa tcgcttcttg tgacttcaat agataaccgt tgtaagaaat ttcgatacca
     7261 ccctcgagtt gcatcttctc taactcttta tcgttgagat agatgtgagc gatctcggcc
     7321 gaagcttcgg ctacgtcccc ttttttgtcg taatatagaa accggggttt ctggatgact
     7381 gcttggcgct gatctttgtc gtaacttgct tcctcgccga gcagttccca tacagtccgg
     7441 ccattctcaa ctttggcgcg gcgaaaatct ttaatactga gagccgactc gggtaactgg
     7501 tccaatagct gaataggatt ttctctaatg tcgcgcagct tattagccca tatttctgcg
     7561 gtgaccttgt agccaactcc cgcaagagaa gcaacgattg cgattagtag taaaaatctt
     7621 cttttacgca tcggataacc aattcagtga attacgaacg taccactctt tgatacttct
     7681 gtaaagcaat aggcatgaaa attgctcagc aatttacaac taatgcaaga agtcgatatt
     7741 gttactattt tttcgcggat gtatccactt attctgcgcc ttgagcaata actccgcgac
     7801 ctcgcgaacc gcgcccctgc caccgggttt ttgggtgatg tagtcgacag accggaatag
     7861 ttcctcagag ccatcgttta ccgttactgc caaaccgacc cggtgcagta cctctagatc
     7921 accgatatca tcgccgatgt aagcgatctg cgtctccgcc aagcctgatt gacgccggat
     7981 cgctccatag gtactggctt tactttgaac cccttggtgc acgattcgaa ggcccagttc
     8041 tttggctcga tatcgcacgg ctttggaatt tcgtgcagtg ataagaccga cctcgatacc
     8101 tgcgcgcaag agtaaaacta tgccttgccc atcgcgcacg tcgaactgct ttgtttccac
     8161 tccccggtcg tcgaggatga tacggccatc ggtcaacaca ccatcgacat cgagcagaag
     8221 caattttata gctttggcct tttttcggac cgctgtagga atgcgcttca cggatgggag
     8281 ttaaag
//
LOCUS       DASUTG010000065         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2085798, whole genome shotgun sequence.
ACCESSION   DASUTG010000065 DASUTG010000000
VERSION     DASUTG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085798"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF089_01420"
     CDS             complement(<1..614)
                     /locus_tag="VF089_01420"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172644.1"
                     /translation="MDVLSTTGYQRRPPHLVAEYKGFFAKEGLEVRFHETTYAPDHNR
                     GMAEGRWDFTLSSADTMIARTTTDGIDYLLFMQAEEGLSAFLIGQSEITSIEQLRGEL
                     LAGDPGDSNLDLIRKKILRRHGMDDDQYEIEIIGSSPKRLEAFLNGRVAAAMLTPPAS
                     DKALAAGGVLLANAEDYVPGWPLTCGWTLRRWLLEHRDLAVRFIR"
     gene            725..1399
                     /locus_tag="VF089_01425"
                     /pseudo
     CDS             725..1399
                     /locus_tag="VF089_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(1441..2415)
                     /locus_tag="VF089_01430"
     CDS             complement(1441..2415)
                     /locus_tag="VF089_01430"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172645.1"
                     /translation="MVIVALWVTSSNAQERPTILAGHGTLSGSILPLWVGTDAKLFEK
                     HGVQVRPIYLPRAAGRPALVSGDIQVYFSAGPPVVQMRLSGGDVAVTSCVVHKLTSKV
                     MVHPAIQTIADLRGKVVAVANPGSASDFAAKLFMARQRMRPGQDVSIIYSGSTSAAFA
                     ALANGRVHAIFASPPNDIQAVAAGFKPLLELGDLNIPYAGNCTAVLRSYAAKQPARMR
                     SFVAGITEAVAYIRRRPSEARSILQKYTRVSDKAILQHTYDSEVQYMEAVPAPTAEGV
                     KSILEHLGISGQPAEAFIAEFIDNRFMKQLIDDGLLNQIYPGGVPTRG"
     gene            complement(2709..3092)
                     /locus_tag="VF089_01435"
     CDS             complement(2709..3092)
                     /locus_tag="VF089_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6172646.1"
                     /translation="MAKILIVDDNPDVREVLRCQLEMLGYLVVSAENGYMGIAKATVE
                     QPDLILMDIMMPEMDGWQASRAVRANPETKDIPILAATAMCRRADLNACLEAGCNGYI
                     VKPFTIMELQQKIGELIANSGENHS"
     gene            complement(3241..4245)
                     /locus_tag="VF089_01440"
     CDS             complement(3241..4245)
                     /locus_tag="VF089_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6172647.1"
                     /translation="MTSPLFSFFTNRSRRVVETVTPARTLADVVLPPQTRRTLEHALA
                     QVRNHELIFGRWGLGERHTAGRGLAFNFAGAPGTGKTLCAEAIAHELGMKLLVVDYAQ
                     AESMWFGETPKNIVAAFRAAAEQSAVLFFDEADAIATRRSAGAILPHDRESNLTINVL
                     LRELEAFNGIVIFATNLAANFDPAFERRIRTHVLFDMPGVEERARIWELQIHPKKTPL
                     RADVDFRRLAERYEMSGGDIKNAVIKAAAAAAAESGPDLGKQIGQEHFETAAQDVLSA
                     KSVMQQSLFAEGGLAVTDPMSVWRKVETRWRTAIVVALGLGGTALIAALAAVALALLR
                     "
     gene            complement(4406..4786)
                     /locus_tag="VF089_01445"
     CDS             complement(4406..4786)
                     /locus_tag="VF089_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172648.1"
                     /translation="MTSWENDLVTDLEKIGEAEVRTRLARGEFGMVGSSKSTAVKAWL
                     NSKESERRDARESKAMANAEQAYCIAREAAGAAAEATSDSREAITLSRLAATNSRRAN
                     VIAIVAMVCSILAVMTAVITVILK"
BASE COUNT         1226 a         1570 c         1438 g         1217 t
ORIGIN      
        1 cgtataaagc gcacggccag atcgcgatgc tcaagtagcc agcggcgcaa tgtccagccg
       61 cacgtcagcg gccaacctgg aacgtagtct tccgcgttgg cgagaagcac tccgcccgct
      121 gccaacgcct tgtcggaagc cggcggcgtc aacattgcgg cagcgactct gccgttcaga
      181 aaagcctcca accgtttggg cgagctgccg ataatttcga tttcatattg atcatcgtcc
      241 atgccgtgac gacgaagaat cttcttgcga atgagatcga ggttcgaatc gccgggatca
      301 ccggcaagca gttccccgcg cagctgctcg atggacgtaa tctccgactg cccgatcagg
      361 aaggcgctca acccctcttc cgcttgcatg aaaagaagat agtcaattcc atccgtagtc
      421 gtgcgggcaa tcattgtgtc cgcactgcta agcgtgaagt cccagcgtcc ctcggccatc
      481 ccccggttat gatccggagc ataggttgtt tcatgaaagc gaacttcgag cccttcctta
      541 gcgaaaaagc ccttatactc ggcgaccaaa tgaggcggcc tgcgttggta accggtggta
      601 ctgagcacat ccattgtgtt tccctcccgc ggtcttctcc cttgtatcgc cagatgcggc
      661 aacgctcaag tgtcgagcag cgtgctcatt agacgactgc tcgccggtta agcctgacat
      721 ccaaatgtca cctgttagcg gttccatcaa actgcgcatc catcatttgc tttggcaaag
      781 ccgttgcgct ggcttacgcc cgcgaggcgc caggctggcg atttgcgcgc gcacggaatc
      841 ggagatcaat gagacggttc accagattca agaactaaaa gcggacgcca gaggctggtc
      901 ctgtgatgtc tgcctcgaag attccgttaa agatttcgtc gacaacgtta taagagagtt
      961 cgacgcatcg acgtcttggt gaataacgcc ggagtgatga cccggccagt tccaaccacg
     1021 gaactagaag tgaagaagtg ggattatacg attgcggtaa atttgcgcgg accgtttctt
     1081 gtaacgaagt ccgtgttgcc cgtcatttat caacgtttca tcgatggtgg gacgcggcgc
     1141 gtacgcaaat ttcatcgcct atgcgacatc aaaatgggga ctggaaggat tcactcagac
     1201 cctggccgcg gagacccgtt cttcgaacat ccggatcaac tccgtagaac caggttacgt
     1261 cgcaacaaag ttaaccggtt acaacggcag tcaacccgaa tcggtaaccg atgtttttgt
     1321 ttatctggca tccgatcaag caaacggcat cacaggcaag atgctgagca gctcaggatg
     1381 gaagtctcag atgaaataac cgcccctgca cttctgtgtg actcagctgg agtgtacaca
     1441 tcatcccctc gtcggcaccc cgcccgggta aatctggttg agcaagccat catcgatgag
     1501 ctgcttcatg aagcggttgt cgataaattc agcgatgaaa gcctcggccg gctgcccgga
     1561 gattcctaga tgctcgagaa tgcttttcac cccttcggca gtgggagctg gcacggcctc
     1621 catgtactga acttcggaat cgtaagtgtg ttgcaggatc gctttatcag aaacccgggt
     1681 atatttttgt aagatgctcc tggcttcact cggacgcctt ctgatataag cgaccgcctc
     1741 ggtgatgccg gcgacaaagc tgcgcatgcg agccggctgt ttcgccgcgt aagaacgcaa
     1801 caccgcagtg caatttccgg cataggggat gttcagatcg cctagctcca gcaacggctt
     1861 gaaacctgcc gccacagcct gaatatcatt ggggggtgac gcaaagatgg cgtgcactcg
     1921 gccattggcg agggcggcaa aggcagcgct cgtcgagccg ctatagatga tcgaaacgtc
     1981 ctgcccaggc ctcatgcgct ggcgcgccat aaacagtttc gcggcaaaat cactggcaga
     2041 acccgggtta gctaccgcca caactttccc ccgcagatcg gcaatcgttt gaattgcggg
     2101 gtgcaccatt accttggacg tgagcttgtg aaccacacag gaagtaaccg ctacgtctcc
     2161 cccgctcaag cgcatctgca ccacaggcgg cccggcggaa aaatacacct gtatgtcgcc
     2221 gctcaccagc gccggcctgc ctgcggcgcg cggcagataa atcggcctga cctggacgcc
     2281 atgcttctcg aacagctttg cgtcggttcc gacccagagc ggcaggatcg atccagagag
     2341 ggttccatgt ccggcgagaa tggtgggccg ttcttgagca ttggacgatg tcacccacaa
     2401 agcgacgatg accaacgcaa ccagttgttg ccgtaaaagc ttgcgtttca gtctcgtatc
     2461 aacctcctgt agagcgatgt ttcaggcgag cacggtcgaa acgtgattac gattaagcgc
     2521 caagtagaat acacgcagac atctcactgc ctggattgtc accagtttgc aagcggtcca
     2581 gcctctggat tggtagtttg tttatcatga aatcaactga ggtggtcaac tcggcggcga
     2641 atccaaatta ttaggtcgtt cgacccaggt gtttgcccta gatttcttag cagaacaacg
     2701 ccggagcgtc aggaatgatt ctcgccgcta tttgcgatta gctcaccgat cttttgctgc
     2761 aattccatga ttgtaaatgg cttgacgatg tagccgttgc aacccgcttc caggcaagca
     2821 ttgaggtcgg cacggcgaca cattgccgtc gccgcaagta tcgggatgtc cttggtctcg
     2881 ggattggccc ggacagctcg gctcgcctgc cagccgtcca tctccggcat catgatgtcc
     2941 atgaggatca ggtccggctg ctccacagtc gcttttgcaa ttcccatgta accattctcc
     3001 gccgatacca ccaagtagcc tagcatttcg agctgacagc gaagtacttc gcgtacgtcc
     3061 gggttgtcat cgacaatcaa aattttggcc atgctaagta ctccggcggt gctctcggaa
     3121 catgccgggg tactattatc gcctaagatt gttgcctggc gcaaatgatt cagatactta
     3181 catgcgggat ctcggctgat gtctcagagg atgatgcgtg cttggctgcg ctcagatgga
     3241 ctaacgcagg agggccaatg cgactgccgc cagcgctgca atcagggcag tgccccccag
     3301 ccctaaagcc acgacaatgg cggtccgcca gcgcgtctct acctttcgcc acacgctcat
     3361 gggatcggtt accgcgagac cgccttcggc aaagagcgac tgctgcatga ctgacttggc
     3421 cgagagcacg tcttgagcgg cagtttcaaa atgctcctgg cctatttgtt tgcctagatc
     3481 aggtccggat tcggccgcag ccgctgccgc tgctttgata accgcatttt tgatgtcgcc
     3541 accgctcatc tcgtaacgct cggcgagccg gcggaaatct acatctgctc taaggggagt
     3601 cttcttaggg tgaatttgca gctcccagat acgggcgcgc tcctccactc ccggcatatc
     3661 aaacagcaca tgggttcgga tgcgccgctc gaatgccgga tcaaagttcg ccgctaggtt
     3721 ggtcgcaaag atcacgatgc cattgaaagc ctctagctcg cgaaggagaa cgttgatggt
     3781 caagttactc tctcgatcgt gcggcaaaat ggcgccagcc gagcggcggg tggcgatggc
     3841 atcggcttca tcaaaaaaca gcaccgcgct ttgctcggcc gcggcgcgaa atgccgcgac
     3901 tatgttttta ggcgtttcgc cgaaccacat cgactctgct tgagcgtagt cgacaacgag
     3961 aagcttcatt ccgagttcat gtgcgattgc ctcggcgcag agggtcttcc ccgtcccagg
     4021 cgccccggca aaattgaacg ctaaaccgcg tcccgccgtg tgccgctcac ctaagcccca
     4081 gcggccgaag atcaattcgt gatttcgcac ttgagcgagc gcgtgctcca atgtccgccg
     4141 ggtttgcgga gggaggacga catctgccaa agttcgtgcg ggggtgacgg tctccacaac
     4201 tcgccgcgag cgattagtaa agaacgaaaa cagaggacta gtcatgattg tttctcatca
     4261 aggcgcaagc gatgtttgcc gacttcttca cacccgctca tggacttaag tatgtaccgg
     4321 ttccacggat agcgttgtgc cgtcttttca cacgaaatcg ttgataccgg attatcgccg
     4381 tcatcatgta aaatcctgtt gcccgctatt tgaggatcac ggtgattaca gcagtcatca
     4441 cagcgaggat actgcaaacc atcgcaacga tagctataac atttgcacga cgtgaattag
     4501 ttgcggccaa ccggctgagt gtgattgctt cccgagaatc agacgtagcc tcggcggccg
     4561 ccccagcggc ttcacgggcg atgcaataag cctgttcggc attcgccatc gctttgctct
     4621 ctcgagcgtc gcgacgctcg gattccttgg aattcaacca tgccttgacg gctgtcgatt
     4681 tagagctccc aaccattccg aactcccctc gtgccagccg ggtacgcact tccgcttcgc
     4741 cgattttttc caaatccgtc actagatcgt tctcccagga agtcatcggc tcatccgctt
     4801 caaacagcca agcttaccaa aaaagccgtc cagccacatc agctgatgcc gttcagttac
     4861 caatccatcc tagcgccggc tttcagcaat tttcttccta tacgcaagaa acctgcgccg
     4921 gttattcgca atcatggaaa cactctgcat aacagcttcg cgatcgtggg atcacggctc
     4981 gataatccag caagcaaatc agccgcattc agccgcaccc cggatcatga ctctcgcgct
     5041 gagaataagg cacagttatt gccctgctat cagtcatgag gaaaccggag agcaaaggct
     5101 ttctgcatgg aggactctat ggcacatgaa acggaactgt tgtatgtcgt gacactggtg
     5161 atccttatct tggtctgcga tctgatcatg aactgtacgg actccgggcg gaagggcaat
     5221 tctctcaact agcgatgact ggcgacgtct catcatctgt cgtattgagc cgagaaaaag
     5281 catgagacat tcgaacccga gaagtatctg gccatctccc cgcgatcaac cactttgctt
     5341 acgaagccgg ttatcttgta agagatggcg ggctgggttc agcataacac agctcaaact
     5401 cctgaaacct actccctgcg cccgcccact gggcggaaat gatgctgctc c
//
LOCUS       DASUTG010000066         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_751755, whole genome shotgun sequence.
ACCESSION   DASUTG010000066 DASUTG010000000
VERSION     DASUTG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751755"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF089_01450"
     CDS             <1..305
                     /locus_tag="VF089_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX6172649.1"
                     /translation="HAALRSYYLLRRIGGVRIVSPTLDGLAVGNSTPVVYCQNNLGGA
                     WERDQLGKWTNACTPGGEEQRRDAFHLGINLILYAMTENYKEDLIHVPFIRKRLTR"
     gene            308..>2564
                     /locus_tag="VF089_01455"
     CDS             308..>2564
                     /locus_tag="VF089_01455"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6172650.1"
                     /translation="MKDLYLTNSVPWWLVALVVAASVALLVQQFLSLRRRLSIGQSIF
                     LVSLRACVYGLLIFFLLSPALVEKQVTQLRRPLTLLIDDSQSMTFPANPGGAHQGKAP
                     SRLDLVKHKLTEGQEPLIQKLSRDYDLRIYRFGTSLEPIAPESVSQLKAQDQGTQLLE
                     LLQVAAKEAGPQSGIVIFSDGIANGDRQSLEGSSALPVPVFTVGAGDPEGFTDARVTD
                     VRTPEFAFRGREFKFDLTVRASGMKGKTLPLYFNRGSGLITSRTVTIDDDDFTRKITL
                     SFTPKDIGPHHFSLNIPAQAGEQISQNNHKDFKVDVQRDKIRVLTLSGSPAWNYRFLR
                     MAMKQDPLIELVSFVFLRTPTDSVDVPDSRLSLIPFPIDDIFLEELKNFDVIFFDDFS
                     HRAYFNPVYLDKVKEFVRDGGGLAMLGGVRSFDSGGYGESALKEVLPVEMDGKGTYRN
                     DSSVPPALTAAGKAHPITRLFPDPRANESAWARTPPLTSINQVRAARGQTLLVAGGDG
                     AAKGSPLLTVGRFGKGRTLALMSDDIWRWNFIAVGNRESPQNHLKLIRQSVRWLAQEP
                     SFEQVKIQPIPLARPGEKLAIKLRVLKDDFTPTRQAAVQVRVFGPEGEPSLVTAKADG
                     AEGEYTGEYTPTREGSYRVEAEANLGGKPLGKDRSSFSAAFPYGEIDDGRPRTELLQA
                     IAESTQGFYFPIADWNEKAVEQVAAKLEQHAPSQIVERRQTQLWSTLWTFLPILLLLC
                     VEWWMRRKWGLL"
BASE COUNT          553 a          732 c          740 g          539 t
ORIGIN      
        1 ggcatgcggc gctgaggagc tactatcttc tgcgacgcat cggcggcgtc agaatcgtca
       61 gtcccactct cgatggattg gcggtcggca attccactcc ggtggtctat tgtcagaaca
      121 acctgggcgg cgcatgggag cgcgatcagt tgggcaaatg gacgaatgcg tgcaccccgg
      181 gcggcgagga acagcgccgg gacgcgtttc acctcggcat caatttaatc ctctacgcca
      241 tgaccgaaaa ttataaggaa gatttgattc acgttccgtt tatccgcaag cggctgacgc
      301 gctgatcatg aaagatctct accttacaaa cagtgtaccg tggtggctgg ttgcgctggt
      361 tgtcgccgcg agcgtcgctt tgctggtcca gcagtttctc agcctgcgac ggcgcctgag
      421 cattgggcag agcatttttt tggtttctct gcgcgcctgc gtttatggtc tgctgatttt
      481 ctttcttttg agcccggcat tagtagagaa acaggtgacg cagctgcggc gaccgctcac
      541 gttattgatc gacgattcgc agagcatgac atttcccgcc aatcccgggg gcgcgcacca
      601 aggaaaggcg ccgagccgcc tcgatctcgt caaacataaa ttgaccgaag ggcaagaacc
      661 gctgattcag aaacttagcc gcgactatga tctacgaatt taccgtttcg gcacgagcct
      721 ggaaccgatt gcgccggaat cggtgtcgca gctcaaagcg caagaccagg ggacacagtt
      781 actggagctg ctgcaagtcg ccgccaagga agccggcccg caatcgggaa ttgtgatttt
      841 cagcgacggt atcgccaatg gcgatcggca aagcttggaa ggaagcagcg cgttgccggt
      901 accggtgttt accgttggcg ccggagaccc agagggcttc accgacgcgc gcgtgaccga
      961 cgtccgaact cctgagttcg cgtttcgcgg gcgtgaattc aagtttgatc tcaccgtgcg
     1021 cgcatcaggt atgaaaggaa aaaccctacc gctttacttt aatcgcggca gcggcctgat
     1081 caccagccgg actgtcacca tcgatgatga cgatttcacg cggaaaatca ccttgagctt
     1141 cactcccaaa gatatcggcc cgcatcattt ttcattgaac atcccggccc aggcaggcga
     1201 gcaaatcagc cagaacaatc ataaggactt caaagtcgat gttcagcgtg acaagattcg
     1261 cgtcctcact ctatccggtt cgccggcctg gaactatcgt ttcctgcgga tggcgatgaa
     1321 gcaagacccg ctgatcgaac tggtctcctt cgtgttcttg cgcacaccga cagatagcgt
     1381 cgatgtgcct gactcgcggc tgagtttgat cccgtttcct atcgacgaca tatttctcga
     1441 ggaacttaag aacttcgatg tgatcttctt tgacgacttc tcgcatcgcg cttatttcaa
     1501 tccggtctac ctggacaaag taaaagagtt cgtccgcgac ggcggcggcc tggcgatgct
     1561 cggtggcgtg cgctcattcg acagcggcgg ttatggcgaa agcgcgctca aggaagtgct
     1621 ccccgtcgaa atggacggca agggaaccta tcgcaacgac agcagcgtgc cgccggcgtt
     1681 gaccgctgcc ggaaaagctc atccgattac gcggctattt cccgatcctc gcgctaatga
     1741 aagcgcatgg gccagaacac cgccgcttac ctcgatcaac caggtgcgcg ccgctcgcgg
     1801 ccaaaccctg ctggtggccg gcggcgatgg cgccgccaaa ggatcgcctt tgctgacggt
     1861 cggccgcttc ggcaaagggc ggactctagc gcttatgagc gatgacatct ggcgctggaa
     1921 tttcatcgcg gtcggtaatc gagagtcgcc gcagaatcat ctgaagctga ttcgccagtc
     1981 agtccgttgg ctggctcaag aaccttcctt cgagcaggta aagatccaac ccattccgct
     2041 ggcgcgtccg ggagaaaaac tggcgatcaa gctgcgcgtc ttgaaggatg atttcacccc
     2101 gacccgccag gccgccgtgc aggtgcgcgt gttcggacct gaaggcgagc ccagcttggt
     2161 cacagccaag gcggacggcg ccgaaggcga atataccggc gaatacacgc ctacaagaga
     2221 aggttcttac cgggtcgaag cagaggctaa cctcggcggc aagcctctgg gaaaagaccg
     2281 atcgagcttc tccgcagcct ttccctacgg tgaaatcgac gatggcaggc cacggaccga
     2341 gctcctccag gcgatagccg aatccaccca gggattttat tttccgattg ctgattggaa
     2401 tgaaaaggct gtcgagcagg tagcggcaaa gctggagcag catgcgccct cgcaaatcgt
     2461 tgagcgtcgc cagacgcaac tctggagtac gctgtggact ttcttgccga tcctgctgct
     2521 gctctgcgtc gaatggtgga tgcgacgcaa atggggtctg ctat
//
LOCUS       DASUTG010000067         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1937977, whole genome shotgun sequence.
ACCESSION   DASUTG010000067 DASUTG010000000
VERSION     DASUTG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937977"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF089_01460"
     CDS             <1..356
                     /locus_tag="VF089_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172651.1"
                     /translation="LYSFHDLLCLKVMKDLTHRGLSLRKVRRCLQPLRRYSGEHGRAL
                     ESLRYLTDGEKLFVITSDREKIFDAIGRHFVFSVGIGHLVRELNSSLRQATAATGRAS
                     ARTMRRFIEKQAGST"
     gene            363..2951
                     /gene="mutS"
                     /locus_tag="VF089_01465"
     CDS             363..2951
                     /gene="mutS"
                     /locus_tag="VF089_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX6172652.1"
                     /translation="MEPAKITPMMQQYLHIKERYHDAILFFRLGDFYEMFFEDAQTAS
                     RILDIALTSRHKSEESPVPLCGVPYHAAEPYIQKLLDAGHKVAVCEQVEDPKTAKGVV
                     QREVVRVITPGTITAAEALDARGNNFLIAVCQDNRNFGLAVTDITTGEFRCTQFADEQ
                     SLFEEIGRIAPSEIIAPDNGAGLLRLLRKEFSNLHITAAPEEFFSGEAARKVMAAGEA
                     KDRGSARHQGVFAASGIISYLAANAGEFLKVIGGLEFYVASHYLVLDENTRRNLELVT
                     GNDGTRKGSLLSVIDRTETPMGARRLRQWLFYPLLDVGAIRERQAGVQALVENYRLRE
                     DLKSALQNVQDLERLAARTVAGTASPRDLVAIKQTLLALADLRRLLAGENTAILRALY
                     DQLSELPEIVQLIGAAIVDDPPFSLKDGGYIRQGFDPELDEIRSGRTRAKEWIAAFET
                     AERRRTGINSLKVRYNRVFGYYIEITSSNLQAVPGDYIRKQTLANGERYITPELKEYE
                     TRVLNSQSLLEKMEAELLGRVRGQVAGNFPVLKKSSLALACIDVLLSLAEVAESCHWV
                     APSVDDGLILLVREGRHPVVEAAIGRNAFVPNDCAIDPKEQQILLLTGPNMAGKSTYM
                     RQIALITILAQMGSFVPAREARIGLVDRVFTRIGASDSLARGESTFMIEMKETAAILE
                     HATQRSLILLDEVGRGTSTFDGISIAWAVAESLHERAERPRTLFATHYHELTDLARTK
                     ERIKNYNFAVKEWQGDIIFLRNLIEGAASRSYGIHVARLAGLPAPVIDHAKSILARLE
                     GGADECRGRSASMENYSGDQPLQMTLFASAEDRFRDQLRSIDVSTITPVEALNILFKL
                     TEDAKK"
     gene            2973..4319
                     /locus_tag="VF089_01470"
     CDS             2973..4319
                     /locus_tag="VF089_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6172653.1"
                     /translation="MTKRSFRYTAIAMAVVALLGMSLVQFDSLAAAQNNKKNHAASPS
                     KSTKVDIPVSRARVQPSGEELKPGSQVSTDGTDKSLVSSVRFISTSRYTRVLLDLSKE
                     AKYAIHRLDGDKSKGQAPRTYIDISGARLLTNSKEPVAVGDRLLRQIRVGQYSDDVVR
                     VVLDLTDDTAAYDISLMPDLYRLVIHIQPQRAGETVDAGADKPVIKATARTRQPAKTT
                     VSGIRKIVLDPGHGGKDPGAIGVGGITEKDLVLSIAKKLAVKLTHELGIQVILTRKDD
                     RYVPLEDRTATANAEDADLFVSLHMNASPNLEARGLETYYLDNTSDEASIRLAARENS
                     TSRKNISDLQFILSDMMQNVKLEDSISLAHHLQGAAVSGMSRKMSDVKDLGVKKALFY
                     VLVGARMPSVLVEMFFITNRLEGPAMNDERHQDAMVDALLEGIRKYNQSMVATKTL"
BASE COUNT         1026 a         1212 c         1284 g          977 t
ORIGIN      
        1 ggctttactc tttccacgat ctgctgtgcc tcaaggtgat gaaggatctg acgcatcgag
       61 ggctgagctt gcggaaggtg cggcgctgtt tgcaaccgtt gagacgctat agcggcgaac
      121 atgggcgagc tctggaatcc ctcagatatc tcaccgacgg cgaaaaactt ttcgtcatca
      181 ccagcgatcg ggaaaagatt ttcgatgcaa tcggtcggca cttcgtattt tccgtcggca
      241 tcggccatct ggtacgcgag ctgaatagct ccctgaggca ggctaccgcc gcaaccggcc
      301 gagcaagcgc gaggacaatg cgtcggttca tcgaaaagca ggccggttcc acctgaccaa
      361 cgatggagcc cgcgaaaatc accccgatga tgcagcagta cctccacatc aaagagaggt
      421 accacgacgc gattctgttc ttccggctcg gtgatttcta cgaaatgttc ttcgaggacg
      481 cgcaaaccgc gtcccgaatc ctcgatatcg cgctaacctc gcgccacaag agcgaagagt
      541 caccggtgcc gctttgcggc gtgccttatc atgcagccga accgtatatc cagaaattac
      601 tcgatgccgg gcataaggtc gccgtttgcg agcaagttga agatcccaaa acagccaaag
      661 gagtcgttca gcgtgaagta gtccgcgtca ttacgcccgg caccattacc gcggcagaag
      721 cgctcgatgc gcgcggcaat aacttcttga ttgccgtatg ccaggataat cggaatttcg
      781 gcttggcagt aacggatatc accaccggcg aatttcgttg cacacaattt gcggacgagc
      841 aatccttatt cgaagagatc ggccgtatcg ctcccagtga aatcattgcg ccggacaacg
      901 gcgccgggct gttgagattg ctgcgcaaag aattctccaa ccttcatatc acggctgcgc
      961 cggaagagtt tttttccggg gaagcggcgc gcaaggtgat ggcagccggg gaggctaagg
     1021 accgaggcag cgcccggcat cagggtgttt ttgccgcttc aggaatcatc agctatttgg
     1081 cagccaatgc cggcgaattt cttaaggtga ttggcggcct ggaattctac gttgcgtccc
     1141 attatctggt gcttgacgag aacacccggc gcaatctcga gctcgtcacc ggtaacgatg
     1201 gaactcgcaa aggctcgcta ctctcggtca tcgatcgaac tgaaactccc atgggcgcgc
     1261 gccggctgcg ccaatggctc ttttaccctc tgctggacgt tggcgccatc cgcgagcgac
     1321 aagcgggtgt tcaggcgttg gttgaaaatt accggttgcg cgaggattta aaaagcgcct
     1381 tgcagaatgt acaggatctg gagcgtctgg ctgcgcgcac cgtcgcgggc acggcatcgc
     1441 caagggattt ggtggcgatt aaacagacgc tcctcgcttt ggcggatttg cggcgcttat
     1501 tggcggggga gaacaccgct attttgcgcg cgctctacga tcaactttcg gagttgcccg
     1561 agatcgtcca actcatcggc gcggcgattg ttgatgatcc gcctttttca ctgaaagacg
     1621 gcggttacat tcgccaaggt ttcgacccgg aactcgatga gattcgcagc ggcagaactc
     1681 gagccaaaga atggattgcc gcattcgaaa cagccgaacg gcgtcgcacc ggtatcaact
     1741 ccctcaaagt gcgctataat cgcgtgtttg gctactacat cgagattacc agctcgaatt
     1801 tacaggcggt gcctggggat tatattcgta aacagacttt ggccaatggc gaacgctata
     1861 tcacgccgga attgaaggaa tacgaaacca gggttcttaa ttcccaaagc ctgttggaaa
     1921 aaatggaagc cgaattgctc ggacgtgtcc gcggccaagt cgcgggcaat ttccctgtgc
     1981 tgaaaaaatc cagcctggcg ctggcttgca tcgatgtttt gctctcgttg gctgaagtcg
     2041 ccgagtcctg ccattgggtt gcgccctcgg tggacgacgg attgatcctg ttagtgcgtg
     2101 aaggccgcca tccggtagta gaagccgcca ttggccgcaa cgcttttgtt cctaacgact
     2161 gcgctatcga tcccaaggag cagcagattt tgctgctgac gggccccaac atggcaggca
     2221 aatcgaccta catgcgccag atcgccttaa tcacgatttt agcgcagatg ggaagctttg
     2281 tgccggccag agaagctcgg atcggcttgg tcgatcgtgt gtttactcga atcggcgcta
     2341 gtgattctct ggcccgcggc gagtcgacct ttatgatcga gatgaaggaa accgctgcca
     2401 ttttggagca tgcgacgcag cgaagtttga tactcctgga tgaagtcggt cgtggtacca
     2461 gcacgttcga cgggatctcc attgcgtggg ctgtcgcaga atcgttgcac gagcgcgccg
     2521 agcgcccgcg gacgttattt gccacccatt accacgagct caccgatctc gcccgcacta
     2581 aagaacgaat caaaaattac aacttcgccg tcaaagagtg gcagggcgat atcatcttct
     2641 taagaaacct gatcgaagga gcggccagcc gcagttatgg cattcacgtc gcccgcttgg
     2701 ccggtctgcc ggcgccggtg attgaccatg ccaaatccat tctcgcgcga ctcgagggcg
     2761 gcgcggatga gtgtcgcggg cgctcggcat ctatggaaaa ttacagcggc gaccagcccc
     2821 tgcagatgac attatttgca agtgccgagg atcggtttcg cgaccaactg cggagtatcg
     2881 atgtttcaac gatcactccg gtggaagccc tcaatatcct tttcaagctc accgaagatg
     2941 ctaagaaatg attccatcgc atgaggtatc tgatgacgaa aagaagcttc cgctacaccg
     3001 cgattgccat ggctgttgtt gctctgcttg ggatgtcctt ggttcaattc gattctttag
     3061 ccgcggctca gaacaacaaa aagaaccatg cggcatcgcc tagtaaatca acgaaagttg
     3121 atatcccagt gtcgcgcgcc cgcgtgcaac cgagcggcga ggaactaaag cccggttcgc
     3181 aagtttcgac ggatggtacg gacaagagct tggtgagttc ggtgcgcttc atctccacgt
     3241 cccgctatac tcgcgtgctg ctcgatctgt ccaaagaagc taaatatgcg attcaccgac
     3301 tcgatggaga taagtcgaaa gggcaagcgc cgcgaactta catcgacatc tcgggggcgc
     3361 gactgctgac caattctaaa gagccggtcg ccgttggtga ccggctcttg cgccagatcc
     3421 gtgtcggcca gtatagcgat gacgtcgtcc gtgtagtctt ggacttgacc gatgataccg
     3481 ccgcttatga tatttcgtta atgccggact tgtatcgact ggtcatccat atccaacctc
     3541 aacgggcagg tgagacggtt gatgcaggtg ccgataagcc cgtcatcaag gcaactgcca
     3601 gaactaggca accggcgaag acgacggtgt ccgggatccg taaaattgtc ttggaccccg
     3661 gccacggcgg caaggatccg ggcgccattg gtgtcggcgg aataacggag aaagatctgg
     3721 tgctgtcaat cgccaagaaa ttggcggtca agctcacaca cgaattgggg atccaagtga
     3781 ttttgactcg caaagacgat cgctatgtcc cgttggaaga ccgcaccgcc acggccaatg
     3841 ccgaggatgc cgatctgttt gtctcgcttc acatgaacgc gagtccgaac ctggaagcca
     3901 gagggctgga gacctactat ctcgacaaca ccagtgatga agcctcgatc cggcttgcgg
     3961 cgcgggagaa cagcacgtcg cgaaaaaata tctccgacct gcagttcatc ttgagtgaca
     4021 tgatgcagaa tgtgaagctt gaagattcga tcagtctggc gcatcacctg cagggggcgg
     4081 cggtgagcgg catgtcgcgg aagatgagcg atgtaaaaga cctgggtgtc aagaaagcat
     4141 tgttttacgt gctagtcggt gcccggatgc cgagcgtctt agtggagatg tttttcatca
     4201 ccaatcggct tgaaggcccg gcgatgaacg atgaacgcca tcaggacgcg atggtcgacg
     4261 cgctgctgga gggtatccgt aaatacaatc agagcatggt ggcgactaaa accctgtaga
     4321 ggtaaactgg ttcgtggact taacctcatt gcctgcctct ttgctcgaac caatcaagga
     4381 tattgccgac ctgcctcggt tgacacgtag ttatgtgcgt acggcgcgta cggccttgtt
     4441 cgagaaacac cgggccggcg gcggcgggct cgaaatcgct gccgcctatt cgaaaatga
//
LOCUS       DASUTG010000068         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_11188, whole genome shotgun sequence.
ACCESSION   DASUTG010000068 DASUTG010000000
VERSION     DASUTG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11188"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..750
                     /gene="def"
                     /locus_tag="VF089_01475"
     CDS             217..750
                     /gene="def"
                     /locus_tag="VF089_01475"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6172654.1"
                     /translation="MSILKVARLGHPVLRKVAEPVSDNELRSDATRQFISDMVETMLE
                     YDGVGLAADQVHVSKQIAVLEVADNPRYPGKPLVPLTVLINPVVTPLSGEFEDDWEGC
                     LSIPDMRGKVPRYKSIRVQALDRNGAPLDFVATGFHARVVQHEWDHLNGKVYLDRMPD
                     LSTLSFITEFARYWLGK"
     gene            805..2097
                     /gene="cofH"
                     /locus_tag="VF089_01480"
     CDS             805..2097
                     /gene="cofH"
                     /locus_tag="VF089_01480"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX6172655.1"
                     /translation="MNFPHLQESLDRLMSKVNPVIGGILDRALSGTDISADEATRLFE
                     ADGADLPVMMLAANQLRTETVGDIVTYVINRNINFTNVCVKACGFCAFSRGHLAEEGY
                     FLPIEEIIRRGQEARELGATEICVQAGLAPGIDGWHYIKLCRALKEALPDIHIHGFSP
                     EEVLYGSTLSGLSIRDYLKALKDAGVGSLPGTSAEILVDDVRRQLSPGRITTAQWIEL
                     IQTAHEVGIPTTSTIMYGHLETAHHKAAHLVILRDIQKNTGGITEFVPLSFVYEEAPM
                     SRRNKIAGLRSGATGSEVMKMYAVSRLMLNKWIPNLQVSWVKEGPKLSQIALLAGAND
                     FGGTLINESISTAAGASYGQLMKPGQFRGLIRQMGRIPAERSTTYKDIRVFETENGSV
                     DLLDQVNDPSERFGSYRNLIALRDFRFREFYSEQKNKH"
     gene            2142..2786
                     /locus_tag="VF089_01485"
     CDS             2142..2786
                     /locus_tag="VF089_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172656.1"
                     /translation="MHGNPASFDAIGNRLGFAIAENDRQRLRLVWHGPRFPAYLCLSI
                     AILLLFISVPIVEAIRIRGFVGPAAALWYFPVMNLILFGIACYLLTLKRTIVFDHQEQ
                     RVVLTNQSLFNRSFLSASYSEIEKINLGVDQVYNGFAVAGSSAAEQFPVPSLRLCLIS
                     GDSVLLDRGSRGRLKKMGTQIGEQLDRPFEIDARPAFDKAGTVKPHEEAERAKI"
     gene            complement(2756..3382)
                     /locus_tag="VF089_01490"
     CDS             complement(2756..3382)
                     /locus_tag="VF089_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172657.1"
                     /translation="MLKIIAIALTLLAGCSFGSGNLQSKHSNDLESRRIRRIAVIPPE
                     TTTAAAHSPAHDNPPEMLAKQLYSAMASLPNWQIVAESEVKQVEQMKPGITDAARLRQ
                     IGELVFADAVMVGRVLRFRERIGDDWGVKSPASVAFVLDLIDVRRGDVIWSARFDETQ
                     KALSENIFGLGDIGQRGVRWLRAEQLAMDGVKKAVGQLHQILARSASS"
     gene            complement(3431..>4451)
                     /gene="aspS"
                     /locus_tag="VF089_01495"
     CDS             complement(3431..>4451)
                     /gene="aspS"
                     /locus_tag="VF089_01495"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6172658.1"
                     /translation="IGIVEGMMVLLFRELKGIDISPPFQRLAWDEAMSRFGSDKPDMR
                     FNLELRDFTDAVRDSQFKVFAAAIAKGGVVKGIRIPKGTELSRKDLDDMTPFVATFGA
                     KGIAWTRITDEGWQSPIAKFLTETEQNEIERIAEAQVGDTILFSADTGRTVCDALGNL
                     RLYLGEKLGLIRPNEYALVWVLDFPLMEYDNDELRYVALHHPFTAPLDEDLSLLDTDP
                     AKVRSKAYDLALNGTEIGGGSIRIHQLELQKKVLGLMGIGAEEAQAKFGFFLEALSFG
                     APPHGGIAFGIDRLAMLLSGAQSLRDVIAFPKSQRAVCMLTEAPSTVDARQLRELGIR
                     TKTAE"
BASE COUNT         1019 a         1225 c         1146 g         1061 t
ORIGIN      
        1 tgcccggcaa gataagcgag cgcgcggact gcataatcca cgcgtcgccc gacgttcatc
       61 aatgaactgc gttcggctct cgccctgggt ctcttcatgc tctgattgaa tatagactcc
      121 ttaggtcttt tatgtcaagc gcgcccaatc aggctcggtt atgtcacacc cttccgcccg
      181 ctccaccgct gtgctatgaa attttgatta gaacgtatgt cgattcttaa agtcgcgcgc
      241 ctgggccatc cggtgctacg aaaagtagcc gaaccggttt cggataacga actccggtcc
      301 gatgccactc gtcagtttat tagcgacatg gtcgaaacca tgctggaata cgacggcgtc
      361 ggtctggccg ccgatcaggt tcatgtgtcg aaacagatcg cggtcctgga agtcgccgac
      421 aacccgcgtt atcccgggaa acctctggtg ccgttgaccg tcctaatcaa tcccgtcgtt
      481 acaccgctta gtggagagtt cgaagacgat tgggaaggct gtctcagcat tcctgatatg
      541 cggggcaagg taccgcgcta caaatccatc agagtccaag ctttagaccg taatggcgcg
      601 cccttggatt ttgtcgccac aggatttcat gcccgtgtcg ttcagcatga gtgggatcat
      661 ctgaacggta aagtttacct cgaccgcatg cccgacttat cgaccctcag ctttatcacc
      721 gagtttgccc gctactggct tggtaaatag ctgccgtggg tccgggtgat tgtctttgaa
      781 aaagtgacag gatagagtcg cgccatgaat tttccacacc tacaggagtc tcttgatcgg
      841 ctcatgtcga aggtcaaccc ggtcatcgga ggcatactcg atcgcgcgct ctccgggacg
      901 gatatttccg ccgacgaagc aactcgactg tttgaagcag acggcgccga tctcccggtg
      961 atgatgttgg cggctaatca actgcgcacc gaaacagtcg gcgatatcgt cacctatgtg
     1021 attaatcgca acatcaattt caccaacgtt tgcgttaaag cctgcggttt ctgcgccttt
     1081 agccgcggcc atttggccga agagggctat tttctgccga ttgaagaaat tattcgccgc
     1141 gggcaggaag cgcgcgagtt aggcgccact gaaatttgcg tgcaggctgg tttagcgccc
     1201 ggaattgacg gctggcatta cataaagctg tgtcgtgcgc tcaaagaggc cttacccgat
     1261 atccatattc acggattttc tcccgaagaa gtgttgtacg gctcgactct ttcgggtctt
     1321 tcgattcgcg actatctaaa ggctttaaaa gatgcgggag tgggaagcct gcccggcacc
     1381 tctgcggaaa ttctggtcga cgatgttcgc cggcaattat ctccagggcg aatcacgacg
     1441 gcgcaatgga ttgagctgat tcagacggct cacgaagtcg gaatcccaac cacatcaacg
     1501 atcatgtatg ggcatttgga aactgcccat cataaggccg cgcatctcgt catcctccgt
     1561 gatatccaaa aaaataccgg ggggattacc gaattcgtgc cgctgagctt tgtttatgaa
     1621 gaagctccga tgtcccggcg gaacaaaatc gccgggcttc gttccggtgc cactggctct
     1681 gaagtcatga agatgtatgc ggtatcgcga ctgatgctga ataaatggat acccaaccta
     1741 caagtgtctt gggtcaaaga aggccctaag ctatcgcaga ttgctttgct cgcaggcgcc
     1801 aatgatttcg gcggcaccct gattaacgaa agcatttcga ctgctgccgg agcgagctac
     1861 ggacaactga tgaagcccgg acagttccgg ggtttgatcc ggcaaatggg gcgcataccc
     1921 gctgaacggt caaccaccta taaggatata agggtatttg agacggagaa tggttctgtc
     1981 gatctgctcg accaagtgaa cgacccgtcc gaacgcttcg gttcttatcg gaacctgatt
     2041 gctctgagag actttcgctt cagagagttt tatagtgaac aaaaaaacaa acattgaaca
     2101 cttcccggcc actcccacat ctctgatcgc tgccgctggt tatgcacggt aatccggcga
     2161 gtttcgacgc catcggcaac cgtctaggtt tcgccatcgc ggagaacgat cgtcaacgct
     2221 tgcgactggt ctggcatggc ccaaggtttc ccgcctacct atgtctgagc attgccattc
     2281 tcctgctgtt catttccgtg ccaatcgtcg aggccatacg catccgagga ttcgttgggc
     2341 cggccgcggc gctgtggtat ttccctgtca tgaacctgat tcttttcggc attgcttgct
     2401 acttgcttac actgaagaga accatcgtat tcgatcacca ggagcagaga gttgttctga
     2461 caaaccagag tctattcaat cgatcattcc tgtctgccag ctattcggaa attgaaaaga
     2521 taaatctcgg cgtcgatcag gtgtacaacg gcttcgccgt cgccgggtca tcagcggcag
     2581 aacaattccc agttccatcg ttgcgcctgt gtctgattag cggtgacagc gtgcttttgg
     2641 accgcggcag ccgcggaaga ttgaaaaaaa tgggcacgca aatcggcgag caactggaca
     2701 ggcctttcga aatcgatgca cgtccggcgt tcgataaagc cggcacggtg aaacctcacg
     2761 aggaagccga acgggccaaa atctgatgta gttgcccaac cgctttcttg acgccgtcca
     2821 tagcgagctg ttctgccctc aaccagcgaa cgccacgctg gcctatatca cccaaaccga
     2881 aaatattttc acttagagct ttctgcgttt cgtcgaatcg cgccgaccag atgacgtcgc
     2941 cgcgtcgaac atcgataaga tccaggacga aggctaccga tgcggggctc ttgactcccc
     3001 aatcatcgcc gatccgctcg cgaaaccgca gtacccgtcc gaccataact gcatcggcaa
     3061 aaaccagttc gccgatttgt cgcagacgcg ccgcatccgt aatccctggc ttcatttgct
     3121 ccacctgctt aacctcgctt tcagcgacaa tttgccaatt cggcagggaa gccattgccg
     3181 aatagagctg tttggcgagc atttcaggag gattgtcgtg agcgggggaa tgtgcggccg
     3241 cagtcgtggt ctcgggggga atcacagcga tacgcctgat ccgacgagat tccagatcgt
     3301 tggaatgctt actttgcagg tttccagatc cgaagctgca gccggcaaga agtgtaagag
     3361 caattgcaat aatcttgagc atgttgattc gttacctcga tgtcaatctt gaacaatcgc
     3421 ctggtcagcg ttattccgcc gtcttggttc gaatacccaa ttcacgaagt tgcctggcgt
     3481 caactgtaga tggcgcttcc gtcagcatgc agactgctcg ctggctcttt gggaaggcaa
     3541 tgacatcgcg cagagattgt gcgccgctta gcagcatagc cagacgatcg atcccgaacg
     3601 cgatgccgcc gtgaggcggg gcgccaaagc tcaacgcttc caggaaaaat ccgaacttgg
     3661 cttgagcttc ttcggctccg attcccatca agccaaggac ctttttttgc aactccagtt
     3721 gatgaatacg gatgctaccc cctccgattt ccgtgccgtt caacgcaaga tcataggctt
     3781 ttgagcgtac ttttgcgggg tcggtatcga gcagagaaag atcttcatcg agtggcgcag
     3841 taaacggatg atgcaacgca acataacgca gttcgtcgtt gtcatattcc atcaatggga
     3901 agtccaggac ccataccagc gcatattcgt ttggccgaat caggccaagc ttttcaccta
     3961 gatagaggcg cagattgccg agcgcatcac agacggttct tcctgtgtcg gcagagaaca
     4021 gaatggtatc acccacctga gcctcggcaa ttctctcaat ctcgttttgt tctgtttccg
     4081 ttagaaattt ggcaatcggc gattgccaac cttcatcagt gattctcgtc catgcgattc
     4141 cctttgcgcc gaacgtcgct acaaaaggcg tcatgtcatc caggtctttg cgcgacagct
     4201 ctgtgccttt cggaatacga attcctttga ccacaccgcc tttggcaatc gccgcggcaa
     4261 acactttgaa ctgtgaatca cgcacggcat cggtaaagtc tcgtagctcc agattaaagc
     4321 gcatatcggg cttgtcggag ccgaagcggc tcatcgcttc atcccaggcc aaacgctgaa
     4381 aaggtgggga gatgtcgatg cctttcaatt ctctaaacag caacaccatc atcccctcga
     4441 cgatgccaat c
//
LOCUS       DASUTG010000069         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1938110, whole genome shotgun sequence.
ACCESSION   DASUTG010000069 DASUTG010000000
VERSION     DASUTG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938110"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(642..866)
                     /locus_tag="VF089_01500"
     CDS             complement(642..866)
                     /locus_tag="VF089_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172659.1"
                     /translation="MPPITVIAATPQLAEKADDLVLTNVEIGNDGEVSCEVANTRNKQ
                     SVTSSYRSFICGVGRMNFTPAKMIPEEQCM"
     gene            1180..1464
                     /locus_tag="VF089_01505"
     CDS             1180..1464
                     /locus_tag="VF089_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172660.1"
                     /translation="MIILSPEGKARTSSERLAALPRFVDLNGKTVGLLDNSKPNNDML
                     QARLEQLLRQNCDVAGVVRRRKLTAQQGAPKAYLDDLAAHADFVISGLGD"
     gene            1778..>2112
                     /locus_tag="VF089_01510"
     CDS             1778..>2112
                     /locus_tag="VF089_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172661.1"
                     /translation="MKQQTFQSERIEIQDELWAANDFFEAKGWSDGLPIVPPTEDRVA
                     WMLSATKRKADEVIGTVPPKWAQATVEKIAINAVMAGCKPEYMPVLIAAVEAITEPKL
                     NLYALQATTG"
BASE COUNT          537 a          575 c          550 g          450 t
ORIGIN      
        1 acttagctaa ggaggcactg accggcgcca ggatctcccc gggcttgcga agcacctgat
       61 agatccagtt gagggcgaac caagctccaa tggcgacaaa gcaccagcgg agcatggtag
      121 ccttcaaccc cgacttcctc atccttcgag actgtcgcct gagatatcga agtgtgccgt
      181 tatacatcag cgccgtcgtc attaagattc accccaaagc attgccagac accttagagc
      241 ttgattatac tttggctcga ggtgggaaca aaattgaaag gctaacggtg tctaccagtg
      301 gacttgccag gtagcagaag cccggccggc cactggcgct ctggcccaac tgggtgcgcg
      361 gctttcgcgg ctcggctacg atcttcgcga tacattcaga atgaatttaa acccgattat
      421 ggcttggaaa ctgccagggt gcattcgccg gcgacggatt agtgctatca ggtactcgat
      481 ggcaaccgcg ttcatgcgaa ccgaagcttc agcgatacac ccgagtcaaa ccaacaactt
      541 taacgccaat ttcgaagtac cggtcttttc ggctgggaac aggcggcgca ggcttatagt
      601 tgaatggcgc gctctggccg gcgctacctc ccggtcgacc gttacataca ctgctcttcc
      661 gggatcattt ttgccggggt gaaattcatc ctgccaacgc cacaaataaa ggatctgtag
      721 ctcgacgtca cggactgttt gtttcgagta ttggccacct cacacgaaac ctcaccgtcg
      781 ttgccaatct cgacattcgt tagcaccagg tcatccgcct tttccgcgag ttgcggggtc
      841 gccgctatga ccgtgatcgg cggcattgtt acctagctca gcacaacact gaggctagcg
      901 gctagagcga aaaattttgc gtttcataac tgcctccgtt cggaccggaa ctgcttgtca
      961 agatagccgc ccgcgctgac ggtaatcaag gcgcaaccca ggcatttgtc ttcgcgccta
     1021 agccatagcc cggtagaaaa ctttttttga ttgcgataag tgaagcttgt atttcagtaa
     1081 gcattaggcg gccggaaaaa atcccaataa gaatccaatc aacgacgaac ggtgacaata
     1141 agatctaaaa aacttgatcg agctgaagaa ggagaaacaa tgattatctt aagcccagaa
     1201 gggaaagcgc gaacatcatc ggaacggctt gccgcactac cacgctttgt agatcttaac
     1261 ggcaagacgg taggtttgtt ggataacagc aagcccaaca acgatatgct gcaagcacgt
     1321 cttgagcagt tactgagaca aaactgcgat gtggcaggcg ttgtgaggcg acgcaaactc
     1381 acagcccagc agggcgcgcc caaagcgtac ctcgacgatc tcgccgctca cgccgacttt
     1441 gtcatcagcg gattgggaga ttgagggtcc tgcacatcgt ggagtatcca cgattctatt
     1501 gaactgcaaa aacgcggcag gctcgcagtc actgttatct ctcacccctt tgaggcgctc
     1561 gccaagtcgg agcgcgaagc tttcggtgct cccgattcgc caattgtgat cgtgcagcat
     1621 ccgatcggga cggtcaaagc cgaagaagtc aaaaaacgag ctgatgccgc ctttggcaag
     1681 ctgctcgaag ctttactcaa ttcgcaccca tcgtcaaatg gagagtctta agcgcttttc
     1741 gtgaagctag ttcgcatacc tttgggaata aaggaacatg aaacaacaaa catttcagtc
     1801 ggagcggatt gaaattcaag atgaactctg ggctgcaaac gattttttcg aagcgaaggg
     1861 ttggagtgac ggactaccca tcgttcctcc tacggaagat cgcgtagcgt ggatgctctc
     1921 ggcgaccaag cgcaaggccg acgaggtcat cggcacagta cccccgaaat gggcccaggc
     1981 caccgtggag aaaatcgcca tcaacgctgt catggcgggc tgcaagcccg aatacatgcc
     2041 cgtcctcatc gctgcagtgg aagcgatcac cgaaccgaag ttgaacctct atgcactgca
     2101 agcaactacg gg
//
LOCUS       DASUTG010000070         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1196858, whole genome shotgun sequence.
ACCESSION   DASUTG010000070 DASUTG010000000
VERSION     DASUTG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196858"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VF089_01515"
     CDS             <1..117
                     /locus_tag="VF089_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6172662.1"
                     /translation="QTLHNIHWFQVIETRGYIENNKIDYWQVIIKLGFRLDD"
     gene            complement(199..1020)
                     /locus_tag="VF089_01520"
     CDS             complement(199..1020)
                     /locus_tag="VF089_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6172663.1"
                     /translation="MTIEPEAFRVFEHAGWEAIPEQYHQAFGGLTTQTIGPLLDALRV
                     KSGTKFLDVATGPGYVAAAAAKRGATVLGIDFSLRMIEQARQLHPGIEFRQGDAEQLP
                     VGNGLFDSAANNFGILHFAQPELALREAWRVLRADGHFAFTAWAQPEDTIGFDIVLRA
                     VEAYGEPRIELPEGPHFFRFSDPAECAGELIGAGFENPTVVKVPQLWRLPSGEALFDV
                     MRNSTVRTAGLLQRQKPVVLQKIRAAICAEAEQYRNGDVIELPMPALIAAGKKPK"
     gene            complement(1162..1887)
                     /locus_tag="VF089_01525"
     CDS             complement(1162..1887)
                     /locus_tag="VF089_01525"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172664.1"
                     /translation="MVFTSWRGVVGVVKPTHRPGSLEEFIRLLPEGIGVLPVYLNFKR
                     GTVDEFRAALNAVEDKVAELANEGVDLIHPEGAPPFMVHGYRGEEKIIRDWEGKYKVA
                     IVTAAQTQVDALHALDVKKILGVTYFVGAVNDITTSYFRDAGFDVLGMEGMSVPFEDV
                     GRLASQEIYAHTRRAFLKHPGADAIYMLGTGWRCLDIIRLLEDDLQVPVIHPVPARVW
                     AVQKRLHVRQPVRGFGRLLLEMP"
     gene            complement(1902..3380)
                     /locus_tag="VF089_01530"
     CDS             complement(1902..3380)
                     /locus_tag="VF089_01530"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172665.1"
                     /translation="MARPERVGTTQSTVSYHGLRDWLDQVEKLGELQKVNGAHWDREM
                     GAITQMLTETGKGKAPAILFDEVPGYPKGYRTLYGQFSTIKRVALTLGLPLEYEHKAD
                     IVKAYHHRMEDMKLLKPKTVKTGPIFDNVLEGDKVDVLKLPVPIHHQQDTHRYIGTAD
                     MCITKDPDAEWYNFGAYRCEVYDEKTIGCQITEGKHGRIHRDKYFERGQSAKFAIVCG
                     QDPLLYLLSASPLPSGVSEYDFAGGIRGEPVEVVKGPFTGFPIPADAEIVIEGEAVPG
                     QVRPEGPFGEWMGYYSDDVVPRPYLDVKTVMYRNDPILTCAPQHKPVDETGLLKGIAG
                     AAEIWKALEACGLPEILGVWNHEGAPATRFTAIQIRQRYPGHARNVLHVAANCMAGAY
                     NGKWTVVVDDDVDCSDMDQVLWAMGTRFDPMTDIDLIQKAWSSGRDPMFLPGNFNNRI
                     LIDACIPYNRKLKGEFPKVVEVPNDVRAQLKAKFPQLFKSQG"
     gene            3576..4058
                     /locus_tag="VF089_01535"
     CDS             3576..4058
                     /locus_tag="VF089_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172666.1"
                     /translation="MPPGNGYDQHLRAIGQSLDNKRITAFELKADAERYVVRGIPEKD
                     SRLLPRLRDWRNRMQGASKETSLVFGTADIDRLDNEGRLNRTKRHRLPDFYSLPNTLR
                     TVGSYLDSKNAELLELHKSPLSVTLLYQNENGHPNMEERSIASFYNLFVALHGKRKDK
                     "
     gene            complement(4115..>5279)
                     /locus_tag="VF089_01540"
     CDS             complement(4115..>5279)
                     /locus_tag="VF089_01540"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172667.1"
                     /translation="GYMRAPGHLQAFFATESHADLVAKRLKIDPAEYRRINFMRDGDK
                     SPLGEVMPHVRPGDTLIKALEISGYRAPKPRNVGRGCAIANWVSKGGESYAFVKIAKD
                     GGITLSSAVTDTGPGAFTIMRQIVGQELKVSPESIRVEMLDTTQVIKDTGVRGSSSTR
                     VHGGAAYEAANRAREQILRIAAQAMNAAPEELILYNGGVTHRRAERRMTFAEIAEANQ
                     GPVVGEGHYANMADGPETSIVAQVAEVGVDTETGEVQVRKLTTAHNTGAILNPLTHQG
                     QIDGGVIMGMGYGIMEDLAVDESGKVLTTNLGEYKIPNIKDVPVLKTAIIQSATGSGP
                     YNSMSIGETALIPTAAAIANAVEDAVGARITSLPITAEKVLNAIRGRPDQQNS"
BASE COUNT         1169 a         1512 c         1371 g         1227 t
ORIGIN      
        1 cagacgttgc acaacatcca ctggtttcag gtcatcgaga ccagaggtta catcgagaac
       61 aataaaattg attactggca agtaatcata aagcttggct tccggcttga tgactgagtg
      121 cctcgatcgt cattcaagtg gtggtttaac ggttgagcta cgacgcgcag ggctctagag
      181 tcgccgccgt catcaaggtc acttcggttt ttttcctgcg gcgatgagtg ccggcatcgg
      241 tagttcaata acatctccgt ttcggtactg ctccgcctcg gcgcagatcg ctgcacgaat
      301 cttctgtaac actaccggct tttgacgctg gagcaatccg gccgtccgaa ctgtgctatt
      361 tcgcatgaca tcaaaaagag cttctccgct tggcaagcgc cacagctggg gaaccttgac
      421 caccgtcgga ttctcgaagc cagcgccgat cagctccccg gcgcattcgg caggatcgct
      481 aaaccggaaa aagtgtggtc cttccggcag ttcgattctt ggctcaccgt aagcttccac
      541 cgctcgaaga acaatatcga acccgatcgt gtcctcaggt tgggcccaag cggtgaaggc
      601 gaagtgaccg tcagcccgga gaactcgcca ggcttcgcgc aacgcgagct ccggctgcgc
      661 aaagtgaaga ataccaaaat tgtttgctgc cgaatcgaat agaccattgc cgaccggtag
      721 ctgctccgcg tcaccctgtc ggaactcgat gccaggatgc aattgtcgag cttgttctat
      781 catcctcaat gagaaatcga tcccgaggac ggtcgcgccg cgctttgccg cggctgcggc
      841 aacatagccc ggacccgtcg cgacgtctaa gaactttgtg ccgctcttta cgcgaagcgc
      901 atccaataga ggtccgattg tctgagtcgt aagcccgccg aaagcctgat ggtactgttc
      961 gggaatggct tcccatccgg cgtgttcaaa aacccgaaac gcttccggtt cgatcgtcat
     1021 aaattttcgg cggatgcaga acagaagttg aacagcccga ctatttctaa gttgagggtg
     1081 tcgacggagt aaggctgcgc ttacctcatc ggacgttctg gatcagcgcg ggagagcata
     1141 ttaccggcac tgagcgtgag tttacggcat ctctaggagc aatcgcccga aaccgcgcac
     1201 aggctgtctc acatgcagcc gtttttgcac tgcccagacc cgggcgggca ccgggtgaat
     1261 caccggaacc tgcaaatcgt cttccagtaa gcgaatgata tctagacagc gccaaccggt
     1321 gccgagcatg taaatcgcat cggcaccagg atgttttaag aacgcccgtc gggtatgagc
     1381 gtagatttcc tgtgaagcga gccgcccgac atcttcaaag gggaccgaca ttccctccat
     1441 gcccagcaca tcgaatccag catcgcgaaa atagctggta gtaatatcgt ttacggcgcc
     1501 aacaaagtat gtgacgccaa gaattttttt gacatccaaa gcgtgtaagg cgtcgacctg
     1561 agtttgcgcc gccgtgacga tcgcaacctt atatttacct tcccaatctc taatgatctt
     1621 ttcttcccct ctataaccgt gcaccatgaa aggcggagcc ccttcggggt gaatgaggtc
     1681 gacgccttcg ttcgccagct cggcaacttt atcctcgacc gcattgaggg ccgcccggaa
     1741 ttcatcgacc gttccccgct tgaagttcaa ataaaccggc aggacgccga ttccttcggg
     1801 cagcaaacgg atgaactctt caagcgatcc gggacgatgt gtcggtttga caacaccgac
     1861 aacgccgcgc caacttgtaa aaaccacctt gtacctctcc gctagccttg tgacttgaat
     1921 aactgtggaa acttcgcctt tagctgcgcg cggacgtcgt tagggacttc caccactttt
     1981 gggaattcgc ctttgagctt ccggttgtaa ggaatgcagg cgtcaatcaa aatgcgattg
     2041 ttgaaatttc ctggcaggaa catcgggtcg cgcccggaag accaggcttt ctgaatcaga
     2101 tcgatatcgg tcattgggtc gaaccgagtg cccatcgccc acagaacctg atccatatcc
     2161 gagcaatcga catcgtcatc gacaaccacc gtccatttac cgttgtacgc gccggccata
     2221 cagttggctg cgacgtgaag cacgttgcga gcatgccccg gatagcgttg ccggatctga
     2281 atggcggtga aacgggtcgc cggcgcccct tcgtgattcc atacgcccaa aatttcaggc
     2341 aacccgcaag cttccagagc tttccagatt tccgctgccc cggcaatgcc tttgagcaaa
     2401 ccggtttcgt ccaccggctt gtgctgcggt gcgcatgtaa gaatcggatc attgcgatac
     2461 attactgtct ttacatccaa atacggacgc ggcacgacat cgtcggaata atagcccatc
     2521 cattcgccga acggcccctc tggtctgacc tgccccggaa ccgcctcgcc ttcgataacg
     2581 atctctgcgt cggccgggat cggaaaaccg gtaaagggtc ctttcacgac ctcgaccggc
     2641 tcgccgcgaa tcccgccggc aaaatcatat tcagaaactc cgctcggcaa aggactggcg
     2701 gaaagcaaat aaagaagcgg atcttggccg cagactattg cgaatttggc gctctgcccc
     2761 cgctcgaaat acttgtcacg gtgaatgcgc ccgtgcttac cttcggtgat ctgacaaccg
     2821 atagtctttt catcgtaaac ttcacagcga taggcgccga agttatacca ctctgcgtcc
     2881 ggatctttgg tgatgcacat atccgctgtg ccaatgtagc ggtgcgtatc ttgttgatga
     2941 tgaatcggca ctggcaactt cagcacatcc accttgtcgc cttccaggac attgtcaaat
     3001 atcggcccgg ttttgacggt cttgggcttg agtagcttca tgtcctccat gcgatgatgg
     3061 taggctttga cgatatcggc cttgtgctca tattcgagcg gcaaccccaa agtcaacgcg
     3121 acccgcttga tggttgagaa ctggccgtac agcgttcggt agccttttgg atatccggga
     3181 acttcatcaa aaaggatggc gggcgcctta cctttgccgg tctcggtgag catctgggtg
     3241 atggccccca tttcccgatc ccaatgtgcg ccattaacct tctgaagctc gcccagtttt
     3301 tcgacctggt ccagccaatc gcgcaaacca tgataggaaa ccgtgctttg ggttgttcca
     3361 actctctctg gacgtgccat tgatcctcct aattggttgg taagtgcagt ggtgccgatg
     3421 tatcggggag cacaacaaac catacgttcg gctctccttg ttgtcaagga agtcggctcc
     3481 ttagcccttg ttttcggaat ttcccgatga tatgacgcgg agcgtctctg cctgcgctag
     3541 tcggcaataa agcctgctgg gaaagtgagg gtttgatgcc acctggaaat ggttatgacc
     3601 aacatcttcg tgcgattgga caatcgctcg ataacaagcg gataacagcc ttcgaactca
     3661 aagctgatgc cgagcgctat gtggtgcgcg gaattccgga aaaggattca cgcctactgc
     3721 cgagattgcg cgattggcgg aatagaatgc agggagcgtc caaggaaacg tcgcttgtct
     3781 ttggaacggc cgacatcgat cgcctcgaca acgaaggccg tttgaatcga acaaagcgtc
     3841 accgcctgcc cgacttctat agtttgccga atactttgag aactgtggga tcttacctgg
     3901 attccaagaa cgccgaactg ctggaactgc acaaaagccc gctaagcgtg actctcttgt
     3961 accagaatga aaacggtcat cccaacatgg aagaaagatc gatcgcttcg ttttacaact
     4021 tgtttgtcgc gctacacggt aaacgcaagg acaagtaagc atcgctcatc tcggctgaac
     4081 gagacaggcg ggctctgtgc cgtcacagat ttttctacga attctgctga tccgggcggc
     4141 ctctgatcgc gttgagcact ttctcagcgg tgatcggcag agaggttata cgagccccga
     4201 ccgcatcttc cacggcgttt gcaatggccg cagccgtggg tatcaacgcg gtctcgccga
     4261 tactcatact attatacggc ccgctgccgg tcgccgactg aatgatagca gttttgagca
     4321 ccggcacatc tttgatattg ggaattttgt attctcccag attggtcgtc agaacctttc
     4381 cgctttcatc gacagcgagg tcttccatga tgccgtagcc cattcccatg attacgccgc
     4441 cgtcgatttg cccctggtgg gttaacgggt taaggatggc cccggtatta tgagccgtag
     4501 tcagctttct aacttgtacc tcaccggttt cagtatccac ccccacctca gccacctgag
     4561 cgactatcga tgtctccggg ccgtccgcca tgttggcgta gtgcccttcc cccactacgg
     4621 gaccctggtt agcctcggca atctcagcaa aggtcatgcg ccgttcggcg cgtcgatgag
     4681 tgacgccgcc gttgtaaaga atcaattctt ccggcgccgc attcattgcc tgggcagcga
     4741 ttcttagaat ctgttcacgc gctctgttgg cggcctcata cgccgcccca ccatgcactc
     4801 gggtgctgct actgccgcgc accccggtat ctttgatcac ctgagtcgta tccagcatct
     4861 cgacccgaat cgactcagga gacaccttga gctcttggcc aacgatttga cgcatgattg
     4921 taaacgcgcc aggaccagta tcggtcaccg ccgaggagag cgtaatgccc ccgtctttag
     4981 cgatcttaac gaaggcgtag gactcgccgc ctttggagac ccaattggca atggcgcagc
     5041 ctcggccgac atttctgggc ttgggagcgc gataaccgga tatctctagg gccttgatca
     5101 aggtatcgcc gggcctcacg tgcggcatta cttcacccaa tggggactta tcaccatcgc
     5161 gcatgaaatt aatccggcga tactcggctg gatcgatttt caaacgtttg gcaaccaagt
     5221 cggcatggct ctcggtggcg aaaaacgctt gcagatgtcc tggagcgcgc atgtaaccg
//
LOCUS       DASUTG010000071         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_604210, whole genome shotgun sequence.
ACCESSION   DASUTG010000071 DASUTG010000000
VERSION     DASUTG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604210"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF089_01545"
     CDS             complement(<1..648)
                     /locus_tag="VF089_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172668.1"
                     /translation="MTPVSTTNDASSPETADLAGGSAVSAEGLWTGVWKQFKRNPIAR
                     ISFYVVCLLFLVALFADFLANNKPYYLSYRQQTYFPIFRSYLVRAGLGQWPKELLNVD
                     FKKLKGAQAIFPPVPYLSTDIDLTAPFENPSPKHWLGTDRLGRDVLAGMIHGSRVSLS
                     IGFVAVGIAVLIGITLGAIAGYFGSWIDLVISRLFEIMLAIPTFFLLITVAALLPP"
     gene            complement(645..1673)
                     /locus_tag="VF089_01550"
     CDS             complement(645..1673)
                     /locus_tag="VF089_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6172669.1"
                     /translation="MASYALQRLLLMIPTFIGVTLIGFFIMRLAPGDPAELRAAGGLG
                     GAGGAGISVEKRMAVDEAMAQWRAQYGLDRPLHVQYGIWMKNLLSLEFGESFKDNQPV
                     WSKIVERLPVTIKLNALSILLVYLIAIPLGIYSATHPNTWSDQALTLFAFVLFAVPLV
                     WAATMAIVFICGGDFLYLFPPGGLESLDYSQQWPFWRKMKDQAWHLFLPVVLMSYDGF
                     AGLSRYMRASMLEVLGQDYVRVARAKGLTEKVVVLKHVVRNSLIPLVTILAGILPGII
                     GGSVIIETIFSIPGLGQLGYESVLARDYPTIMALFSVSAVLTLIGILISDLLLSVVDP
                     RIAFGRRE"
     gene            complement(1683..3455)
                     /locus_tag="VF089_01555"
     CDS             complement(1683..3455)
                     /locus_tag="VF089_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEX6172670.1"
                     /translation="MFRARGLGVLLVCFVLAGCRDQTEITVGDSQEVVNDKQAGTPTT
                     GDWLIIHALSDPEQLNPLTSNDASASTINQNIFQSLLTRDPRSLELKPLIATARPAIA
                     EDKLSYTFKIRRDVNFQDGRPLTGEDVLFSIKAIKCPLVNAPFLRVYFDSVVDAQLID
                     EYSIRFTITEPYFLNESVLGGIDVLPRHYYDPENLLKDVSIADLRKPIDELPAGVKKF
                     ADDFNKNFSRNPMGSGPYTFGSWKTGREIELIRDRDYWGNGKEGIDQPYLDRYRYRII
                     NNPDAALVRLKSGSLDEMGLTPIQHVRGTDSQRFKGQFRKYDYFSPNYSYIGWNNDHP
                     IFRDKQVRKAMTHFTNRNQMVKTILFGLGEVVEGPIYFFRPEYDKTLPSYAYDPAKAM
                     KLLSEAGWRDSDADGVLDKVIDGESVPFRFEIKVNSGNPTRKSVALTLQDELRRYGIA
                     AAVRELDWTIFLGDVKTRKFDAVILGWAMPVSEPDAYQVWHSSQSANNGSNHIAYKNA
                     RVDQILEQYRREFDPQKRIELYREFQQILSDEQPYTFLFVGKSVVAVQRRIRGVEVLP
                     VGLNPIEWWVPRALQRYGDHVAAN"
     gene            complement(3458..4222)
                     /locus_tag="VF089_01560"
     CDS             complement(3458..4222)
                     /locus_tag="VF089_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX6172671.1"
                     /translation="MLAAVFTMIGCATVAPPPAKPKLPAKQWQAGELINSLGERHKQF
                     RSLRALARLDYAGPDGKGNVQEAVLVQRPDRLRLETLTFLGAALIVTVNDREIIGYHP
                     REGIFVRGPRTKENLFRYTQIPLELAEITALLLGLPPIDTGAQLRQEGSVVVFSPNRT
                     KQDAVTFEAQTPVPTKWERFDDAGAVELSAEFLDYISTPAGPFPSRIVIEAHLQKRKL
                     EIHYQHPEINASLPPEIFTQEKPAHAEELRLETLGG"
     gene            complement(4270..>5017)
                     /locus_tag="VF089_01565"
     CDS             complement(4270..>5017)
                     /locus_tag="VF089_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6172672.1"
                     /translation="ATYVELKDDEKALAEFARISDKSDLYLDSRLQMAYLYERTDQVE
                     KAIDSVQLVLRKKTDRKEVYALLSSLYRKAKDFPKAIEAMEHVIALDPKSDQAYFQLG
                     ALYDEAKNKDQSVANMKRAIELNPKNAAALNYLGYTWAEMGVRLDDAEQLIQRALQLE
                     PNDGFYIDSLGWVYFQKGDYTRAVEQLERAVAITVDDPTIIEHLGDAYVKVGKPDRAL
                     LRYREAIQKSKEDDQVKRIRDKIERLEKKI"
BASE COUNT         1144 a         1396 c         1338 g         1139 t
ORIGIN      
        1 cggcggcaac agcgctgcca cggtgatcaa caggaagaac gtcggaatcg ccagcatgat
       61 ttcaaacaga cgcgagatca ccagatcgat ccatgagccg aagtagccgg caatggcacc
      121 caaagtaatt ccgatcagca ccgcaatacc aacggcgaca aatccgatcg acagcgatac
      181 tcgggaacca tgaatcattc cggccaagac atcgcgcccc aaccggtcgg tgcccagcca
      241 atgcttcggc gaaggattct caaacggcgc cgtcagatcg atatcggtgg aaagataggg
      301 taccggtgga aatatcgcct gcgcgccttt gagcttcttg aaatcgacgt tgagcagttc
      361 cttgggccat tggccaaggc cggctcggac caggtaactg cgaaaaatcg gaaagtaggt
      421 ttgttgccga tatgacagat agtacggttt gttgttggcc agaaaatcgg cgaataaggc
      481 gactagaaaa agcaagcaga caacataaaa gctgatgcga gcgatcggat tgcgcttaaa
      541 ctgtttccag acaccggtcc ataagccttc agcgctaact gcgctgccac cggccaaatc
      601 ggctgtttcc ggtgatgaag catcgttggt cgtcgaaaca ggcgtcattc tctgcgaccg
      661 aatgcgatcc gcggatcgac caccgataaa agtagatcgg aaatcagaat accgatcaga
      721 gtcaacaccg cgctgacact gaagagcgcc ataatcgtcg ggtagtcccg ggccaacacc
      781 gactcgtagc ctagttgacc cagccccgga atggaaaaga tcgtttcgat gattacgctg
      841 ccgccgatga tgccgggaag tattccggcc aagatcgtca cgagcgggat caaggagttg
      901 cgtacgacgt gcttcagcac gacaaccttc tctgttagcc ccttggcgcg cgcgactctg
      961 acatagtctt gcccgagaac ttcgagcata cttgcccgca tatagcgaga taacccggcg
     1021 aaaccgtcat aggacatcag caccaccggc aggaataaat gccaggcttg atccttcatc
     1081 tttctccaga acggccactg ctgcgagtaa tcgagggatt caagaccgcc cggcggaaac
     1141 aaataaagaa aatcgccgcc acagatgaag acaatcgcca tggtggccgc ccacaccaac
     1201 gggacggcga acagaacgaa tgcaaacaag gtcagcgcct gatcgctcca ggtattcggg
     1261 tgggtcgcag aatagattcc caacggtatc gcgatcaggt acacgagcaa aatggatagc
     1321 gcgttaagct tgatcgtcac cggcaggcgc tcgacgatct tgctccatac cggctgattg
     1381 tctttaaagg attcgccgaa ttccaagctc aatagattct tcatccagat accatattga
     1441 acatgaagcg gccgatcgag gccgtactgg gcgcgccact gggccatcgc ttcatcgacc
     1501 gccatccgct tctctacgga gatccccgca cctcccgcgc cgcccaggcc acctgcggcg
     1561 cgcaattctg caggatcgcc gggagccagc cgcatgatga aaaatccgat aagagtcacc
     1621 ccgataaagg tgggaatcat caagagaagt cgttgcagcg cgtaagatgc catttcactt
     1681 tgctagtttg ccgccacgtg atcgccgtac ctttgcaagg ctcggggaac ccaccattcg
     1741 atgggattaa gcccgaccgg caagacctcg actccgcgga tgcgcctttg caccgccacc
     1801 accgatttgc cgacaaaaag aaatgtatac ggttgttcgt cgctcagaat ctgctggaac
     1861 tcccggtaga gttcgattct cttttgtgga tcgaactcgc gacgatactg ttcgaggatc
     1921 tgatccaccc gggcgttctt gtaagcgata tgatttgaac cattgttggc cgactgtgac
     1981 gagtgccaga cttgataggc atcgggttcg ctaaccggca tcgcccatcc gaggataacc
     2041 gcgtcgaact ttctcgtttt tacgtcgccg agaaaaattg tccagtccag ctcccgaacc
     2101 gctgcggcga tgccgtatct cctgagctcg tcttgcagcg ttagcgcgac gctcttgcgc
     2161 gtcggattgc ccgaatttac cttgatctcg aaacgaaacg gaacgctttc gccgtcgatg
     2221 actttgtcga gcacgccgtc ggcatcgctg tcgcgccagc ctgcctcgct gagaagcttc
     2281 atagctttgg ccggatcgta ggcataactc ggcaaagtct tgtcatactc cggtcgaaag
     2341 aaatagattg gcccttcaac cacctcgccg aggccgaaca ggatggtttt caccatctgg
     2401 tttcgattgg tgaagtgggt cattgccttg cgcacctgct tatcgcggaa gatcggatga
     2461 tcattgttcc agccgatata gctgtaattc ggcgagaagt aatcatactt gcgaaactga
     2521 cctttgaacc tctggctatc ggttccgcgc acatgctgaa tcggcgtcaa gcccatttcg
     2581 tcgaggctgc cgctcttgag ccgtaccagg gcggcatcgg gattattgat gatgcgatac
     2641 ctgtaacggt ccaggtaggg ttgatcgatc ccctccttcc cgttgcccca gtaatctcga
     2701 tcccggatca gttctatctc acgcccggtt ttccagctgc caaacgtgta gggtccgctg
     2761 cccatgggat tgcggctgaa attcttgttg aagtcgtcgg cgaatttttt gacgccggcc
     2821 ggaagttcgt cgataggctt gcgcaggtct gcgatgctga cgtcctttaa cagattttcc
     2881 ggatcataat agtggcgcgg caaaacatcg atgccgccga gcacgctttc gttcaagaaa
     2941 taaggctccg tgatggtgaa ccggatgctg tactcatcga ttaattgcgc atcgacgacc
     3001 gaatcgaaat agacgcgcaa gaacggcgca ttgaccaacg ggcatttgat cgccttaatg
     3061 gaaaagagca catcttcgcc ggtgagcggc cggccatcct gaaagttaac atcgcggcga
     3121 atcttgaacg tataactgag tttatcctcg gcgatggccg gccgcgccgt ggcgattaac
     3181 ggctttaatt cgaggctccg agggtcgcgc gttaacaggc tctggaatat gttttggttg
     3241 atcgtgctcg ccgaggcgtc gttgctggtg agcggattga gctgttcagg atcgctgagc
     3301 gcatggatga taagccagtc acctgttgtc ggagtgccgg cttgcttgtc attgacaacc
     3361 tcttgagagt ccccgaccgt gatctcggtc tggtcacgac atcccgccaa gacgaaacaa
     3421 actaatagaa ctccaagacc acgcgcgcga aacatcgtta accgcccagt gtctctaacc
     3481 gcagttcctc agcatgagcc ggcttttctt gtgtgaatat ctccggcggt agcgaagcat
     3541 tgatctcggg gtgttgatag tgaatttcga gttttctctt ctgcaagtgt gcttcaatta
     3601 cgatcctcga tggaaatggg ccggccggag ttgaaatata atccaaaaac tcggcactta
     3661 actccaccgc gccggcgtca tcaaagcgct cccatttggt aggaaccggg gtttgcgcct
     3721 cgaatgtcac ggcgtcttgc ttggttcggt ttggcgagaa caccacaacg gaaccctcct
     3781 gccgcagctg cgcgcctgtg tcgatcgggg gcaagcccaa cagtaacgcg gttatttcgg
     3841 ctagttcgag aggaatttgt gtatagcgga acaggttttc cttggttcgc ggtccgcgca
     3901 caaagattcc ctcgcgcggg tgatatccga tgatctctcg atcattgacg gtaacgatca
     3961 aggcggcgcc gaggaaggtc agcgtttcca gccggagccg gtccggcctc tgaaccaaaa
     4021 ctgcctcttg cacattgccc ttaccgtcgg gccccgcata atccaggcgg gctaaagcgc
     4081 gcaatgagcg aaattgctta tgacgctcgc ctagggagtt gatcagctcg ccggcttgcc
     4141 actgtttggc cggaagcttt ggcttggctg gcgggggcgc taccgtggcg cagcctatca
     4201 tcgtgaaaac tgctgccaac atgacgagat tccgcacgcg acctgataac ccgcgccgca
     4261 gagtccccgt caaatttttt tctccaggcg ctcaatctta tcacggattc gtttgacttg
     4321 atcgtcttct ttggattttt ggatcgcctc gcggtagcgc aacaaagcac gatctggctt
     4381 gccaaccttc acataggcat caccgagatg ctcgatgatg gtaggatcat cgacagtgat
     4441 agcgaccgcg cgctccaact gttcgacggc cctggtgtag tcgcctttct gaaagtatac
     4501 ccagccgaga ctatcaatat aaaagccgtc gttaggctca agttgcaacg ctcgctgtat
     4561 caactgttcc gcgtcatcga gccgtacgcc catctccgcc caggtataac cgagatagtt
     4621 aagcgccgcg gcgttcttcg gattgagctc gatggcgcgt ttcatattag cgacgctttg
     4681 gtctttgttc ttggcttcgt cgtacagcgc tcccagttgg aaataggcct gatcactctt
     4741 tggatccagc gcgattacgt gctccattgc ctcaatcgct ttggggaaat ccttggcttt
     4801 ccggtacaag cttgaaagta acgcgtagac ttccttgcgg tcagttttct tgcgcaggac
     4861 caactgaacc gaatcaatgg ctttttccac ttggtccgtg cgttcataaa gataggccat
     4921 ctgcaaacgg ctgtctaaat acaggtcgct cttgtcagaa attctggcaa actcggccag
     4981 cgccttttca tcgtccttga gctcgacata ggtggcg
//
LOCUS       DASUTG010000072         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1790399, whole genome shotgun sequence.
ACCESSION   DASUTG010000072 DASUTG010000000
VERSION     DASUTG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790399"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VF089_01570"
     CDS             <1..1360
                     /locus_tag="VF089_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172673.1"
                     /translation="IGVDLPLHLEAVVANFSDQEIKDLLVQLSIDGKNRDQKLTSVPP
                     RGDATVALQTRLSQAGSHAGLLTIKKDGLAGNASMHFGVHAQDQLRILVVDGDPQTSL
                     VQSETFFLTRALNPAGESDSSQYLPTVIIPEGLSGAALDSYQVIVLCNVATLPDAFVA
                     KLQNYLRGGGGLLIFGGDRLQADHYNAKLAPILPSPIRDKKQGLEATGEKIAKLELTH
                     PALQSLSDNLLQESIKSARVWGYSRILSSGKSVLISLANGDPLLIEHKVGSGRVMFIT
                     TTADRDWSDLPVKTAYLPMIQSLTNYLAGGKRGLLDSGIAVGEVNEISLPPGNVGKTI
                     RITKPNKQPAEVMMSAAQDRAAGSFEDNDVAGIYQLGLSRNDQESGSPTLYAVNPPFL
                     ESRLEEISERELQAKLTPVRADVVSIETLQQGGTRRDLALPLLGLLLATLLLEGWFAQ
                     RI"
     gene            1389..2159
                     /locus_tag="VF089_01575"
     CDS             1389..2159
                     /locus_tag="VF089_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX6172674.1"
                     /translation="MNRRDFLCVLSKTLLAAGLSPAGASASHPKGKTPTQFVFAQIKY
                     RGGDWNPHPLSVSPLMEELMQHTSVEAAAARHEVNLTDRDLFNYPFLYITGRYEFEPF
                     SAEEIETLRLFLSFGGFLLIDDGLGQQGYGFDKSIRREMQRVFPGNEFKRLPNGHAAL
                     RSYYLLRRIGGVRIVSPALDGLTIGNSTPVVYCQNNLGGAWERDQLGKWTNACTPGGE
                     EQRRDAFHLGINLILYAMTENYKEDLIHVPFIRKRLTR"
     gene            2162..>2901
                     /locus_tag="VF089_01580"
     CDS             2162..>2901
                     /locus_tag="VF089_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172675.1"
                     /translation="MKDLYFISSVPWWLVALVVAASGALLVQQFLSLRRRLSIGQSIF
                     LVSLRACVYGLLIFFLLSPALVEKQVRQLRRPLTILIDDSQSMTFPANPGGAQEGNAA
                     TRLDLVKQKLTEGEQPLIQKLSRDYDLRVYRFGTSLEPIAPESVSQLKAQDQGTQLLE
                     LLQAAAKEAGPNSGIVIFSDGIANGDRQSFEGTSALPVPVFTVGAGDPEGFTDARVTD
                     VRTPEFAFRGREFKFDLTVRASGMKGKTV"
BASE COUNT          636 a          815 c          812 g          638 t
ORIGIN      
        1 catcggtgtc gatttgccgc tgcacctcga agccgtcgtg gcaaacttca gtgatcagga
       61 aatcaaggat ctgctcgtgc agttgagcat cgacggaaaa aacagagacc agaaactcac
      121 gtccgtccct cccagaggag atgccacggt tgccctgcaa actcgactgt ctcaagcggg
      181 atcgcatgcc ggacttctga cgatcaaaaa agacggcctc gccggcaatg cttcaatgca
      241 ttttggtgtg cacgctcaag atcagctaag gattctggtc gtcgacggcg atccgcagac
      301 atctttggtc cagagcgaaa cgttttttct cacccgcgcc cttaaccctg ccggggaaag
      361 tgactcgtca caatacctgc ccaccgtgat cattcctgaa ggactgagcg gagccgcctt
      421 ggactcgtat caggtgatcg tcttgtgcaa cgtggctacc ttacccgacg cctttgtcgc
      481 caagctgcag aattatttgc gcggcggggg cggtctgttg attttcggcg gtgaccggct
      541 gcaagccgat cactacaacg ccaagctcgc gccgatcctc ccatcgccta ttcgcgacaa
      601 gaagcaaggt ttagaagcta ccggtgaaaa aatcgccaag ttggaattaa cccatccagc
      661 gctacaaagt ctttccgata accttttgca agaatcgatc aagtcggcgc gggtctgggg
      721 ctattctcga attttgtcgt ctggcaaatc cgtgctgatc tccctggcaa acggggaccc
      781 gctcctgatc gaacacaaag tgggctccgg ccgggttatg ttcatcacca ccacggcgga
      841 ccgggactgg agcgacctgc cggtgaaaac cgcctatctg ccgatgattc aatcgctgac
      901 aaactatctt gccggaggca aacgcggcct cctcgatagt gggattgccg tcggcgaggt
      961 caacgagatc tccttgccgc cgggtaatgt cggcaaaacg attcgcatca ccaagcccaa
     1021 caagcaaccg gccgaagtta tgatgagcgc ggcgcaagat cgcgccgccg gttcatttga
     1081 agacaacgat gtcgcaggca tctaccagct aggcctgagc cgcaatgatc aggaaagcgg
     1141 ctcaccgacg ttgtatgcgg ttaacccgcc gtttctcgaa tcgcgcctcg aggaaatcag
     1201 cgagcgcgaa ctgcaagcca agctcacccc ggttcgcgcc gacgtggttt caatcgagac
     1261 gctgcaacag ggcggtaccc gaagagacct tgcgctgcca ttactcgggc tgttgctcgc
     1321 gacccttttg ttggaaggct ggttcgcgca acgtatttag tttttctgct gcggagaatc
     1381 gattgattat gaacaggcga gattttcttt gcgttctttc caaaactttg cttgctgccg
     1441 ggctgtcgcc ggcgggcgct agcgccagcc atcccaaagg gaaaacccca acgcaattcg
     1501 tctttgccca gatcaaatat cgcggcggcg actggaaccc gcatccacta tcggtaagtc
     1561 cgctgatgga agagctgatg cagcacacca gcgttgaagc agccgccgcc aggcatgagg
     1621 tgaacctcac ggatcgcgat ctttttaatt atccctttct ctacatcacc ggcagatacg
     1681 aatttgagcc tttttccgct gaggagatcg agacgctgcg attgtttctc tcattcggcg
     1741 gctttctctt gatcgatgac ggcctgggtc aacaaggtta cggcttcgac aaatcgatta
     1801 ggcgggagat gcagcgagtt tttccaggca atgagttcaa acggctaccg aatgggcatg
     1861 ccgccctgag gagctattac cttctgcgac gcatcggcgg cgtcagaatc gtcagtcccg
     1921 ctctcgatgg attgacgatc ggcaattcca ctccggtggt ttactgtcag aacaacctgg
     1981 gcggcgcatg ggagcgcgat cagttgggta aatggacgaa tgcgtgcacc ccgggcggcg
     2041 aggaacagcg ccgggacgca tttcacctcg gcatcaattt gatcctctac gctatgaccg
     2101 agaattacaa ggaagatttg attcacgtcc cgtttatccg caagcggctg acgcgctgac
     2161 catgaaggat ctctatttca taagcagtgt accgtggtgg ctggttgcgc tggttgttgc
     2221 cgcgagcggc gctttgctgg tccaacagtt tctcagtctg cgacggcgcc tgagcatcgg
     2281 gcagagcatt ttcttggttt ctctgcgcgc ctgcgtttat ggtttgttga tttttttcct
     2341 cctgagcccg gcgttggtgg agaagcaggt caggcaactg cgccgaccgc tcacgatatt
     2401 gattgatgat tcgcagagca tgacatttcc cgccaacccc ggaggcgctc aggaaggaaa
     2461 cgcggcgact cgcctcgatc tcgtcaaaca aaaactgact gaaggagagc aacctctcat
     2521 tcagaaactt agccgcgatt acgatctaag agtctaccga ttcggcacaa gcctggaacc
     2581 gattgcgccg gagtcggttt cgcagctcaa agcgcaggat cagggcacac agttgctgga
     2641 gctgctgcaa gccgccgcca aggaagccgg cccgaattcg ggaattgtga ttttcagcga
     2701 tggtatcgcc aatggtgata ggcaaagttt cgaaggaacc agcgctttgc cggtgccggt
     2761 atttaccgtt ggcgccgggg acccggaggg cttcaccgac gcgcgcgtga ccgacgtccg
     2821 aactcctgaa ttcgcgtttc gcgggcgtga gttcaagttc gaccttaccg tgcgcgcatc
     2881 agggatgaaa gggaagaccg t
//
LOCUS       DASUTG010000073         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_455845, whole genome shotgun sequence.
ACCESSION   DASUTG010000073 DASUTG010000000
VERSION     DASUTG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455845"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="VF089_01585"
     CDS             complement(<1..1946)
                     /locus_tag="VF089_01585"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6172676.1"
                     /translation="MIVSSKRSTQDWLLLVGLFCFFFSGAAGLVYQVVWTRMLTQIFG
                     NTTYAIATVLSAFMAGLAIGSYLFGKIADRGKNDFLLYGLLEAGVGIYGFAVPWLFAL
                     AQKAYGPIFGLNESYPFLFNLVLFFLSFVLLVFPTLLMGATLPVLSRFYVRSFSQFGR
                     RVGDLYATNTTGAVIGCAAAGFFLIPTLGMRTTVYVAAGLNLIVAAVILVVDRLRDKK
                     PLEYITASAGEIPETPVTDNSPPWLGWVVLVSFGLSGFASLVYENAWTRALTLVIGSS
                     IYSFTTMLVTFLIGLALGGFIYARFLGQREARVSSFGLIEMWVGLAALATIPLFERLP
                     LIFVRLLHGFGDTFTVFLYLQIFLSALVMFLPTVLLGMTFPLVARLFTQSLYRVGSGV
                     GSSYAANTVGAVLGAFAGGFILIPTIGVQNAIIFAVVMNLLIGCVLVASDPKLTPLPR
                     AMLGAAVLILAVLIPFKVQRWDQHILTSGVTIYNDRYESLPSDSLRIEEMKRDDILFY
                     REGLTTTVSVHQISGSDYIYFKSNGKIDGSYGDALSQLMTSYIPMLLHPKAEQALTIG
                     LGSGHSAKALATFDTVKEIEVIEIEPAMIEASKYFDRASVEVEEFPEGVSLPNTPAGR
                     LWYDDQKKRLFYKGVMEDDERSKL"
     gene            2274..>3736
                     /locus_tag="VF089_01590"
     CDS             2274..>3736
                     /locus_tag="VF089_01590"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172677.1"
                     /translation="MAKAEKRDSAPVESVRDLRSAIEWMKKNGDLIETDKEVDPDLEI
                     TGIQKQLDGGCPILFNNIKGKPHHRCITNLFGDMNVIGKLFGWKDHADRTRKLAYALT
                     HPIPPVIIDQKDAPVQEEVYEKPADANEFVVPIRHTDIEPELTVGSGNGLISGEYFDG
                     GSHIGYNRMNFRWGNVGTFQISPGSHSWQVVTKYYKDNKLVPITMNFGLPPSSSLLAG
                     SGFDYVILPQGCDEIGMAGAVQGSPVRLVKARTVDALAVADAEVVLEGYLNPRDRRFE
                     TAESEKAGVQGRYHFHPEWAGYMGKAYKAPTFHVTAVTTRKRESKPIIFPLGVHTLDD
                     HNIDTTVREAAMFELCERMQPGIIMDVNIPYCMTDWGGAIIQVKKRNKIEEGWQRNFM
                     AAILATSQGSRLVIAVSEDTDPYDMDDVMWCLTTRVNPKTDIINPLPGGRGQTFMPAE
                     RMTAGEREWTASNTMFEGGMGIDATVPFGYENDFMRPV"
BASE COUNT          985 a         1004 c          992 g          755 t
ORIGIN      
        1 atgagctttg agcgttcgtc atcttccata acgcctttgt agaaaagacg tttcttctgg
       61 tcatcgtacc agagccgacc ggcgggcgtg ttcggaaggg acaccccctc gggaaattct
      121 tccacctcga cggacgcgcg atcgaaatat ttgcttgctt cgatcatggc cggttcgatt
      181 tcgatgactt cgatctcttt caccgtatca aatgtcgcaa gcgccttagc cgagtgaccg
      241 ctgccgagcc cgatggtaag cgcctgctcg gctttcggat gaagcagcat cggaatatag
      301 ctggtcatca actgactcag cgcgtcgccg tatgagccgt cgattttgcc gttcgatttg
      361 aaataaatat agtcgctgcc ggaaatttga tggacactga cggtggtagt cagcccttct
      421 cgataaaaca aaatatcatc gcgtttcatc tcctcgatac gcagcgaatc cgaaggcaag
      481 ctctcatagc gatcgttgta gatagtgacc ccgctggtca ggatgtgctg atcccaacgc
      541 tgcactttga agggtatgag gaccgccaag attaaaacgg cggcacccag catcgctcgc
      601 ggcaatggcg tgagctttgg atcgctcgcc accagcacgc atccgatcag cagattcatc
      661 accactgcga atatgatcgc attttgcacg ccgatggttg gaatcaaaat aaacccgccg
      721 gcaaaagcgc ccagcaccgc tcctactgta ttggcggcgt aagagctgcc gacgccgctg
      781 cccacgcggt aaaggctttg cgtaaacagg cgcgcgacta agggaaacgt catgcccaag
      841 agaaccgtgg ggaggaacat gacgagtgcc gagagaaata tctgcaagta gagaaagacc
      901 gtgaaagtat cgccaaagcc gtgcagcaat cggacaaaga tcagcggcag gcgttcaaac
      961 agcgggatcg tggcgagcgc cgccaagcca acccacatct cgatcaggcc gaagctactc
     1021 acacgagctt cccgctggcc caagaagcgg gcatagataa accctcccag ggctagcccg
     1081 atcaaaaatg tcaccagcat ggtcgtgaat gagtagatcg agctgccgat caccaatgtc
     1141 agcgcgcgtg tccaggcatt ctcatacacg agcgaggcga agccggataa cccgaacgaa
     1201 accaggacta cccagccgag ccagggtggc gaattgtccg tcacaggtgt ctcaggtatc
     1261 tcccctgcgc tggccgtaat gtattcgagc ggcttcttgt cacgcaaacg gtcgacaacg
     1321 agaatcactg cggcgacaat caaattcaag ccggctgcca catagaccgt tgtacgcatg
     1381 ccaagcgttg gaatgagaaa aaatccagcc gcagcgcaac cgattaccgc tccggttgta
     1441 ttggtcgcgt aaagatcgcc cactctgcga ccaaactgcg aaaagctgcg cacataaaac
     1501 cggctcaaaa ccggcaaagt tgcgcccatc agcaaggtgg gaaaaaccaa tagcacaaac
     1561 gataggaaga agaggactag gttgaacagg aacggatacg attcgttcag tccgaaaatg
     1621 ggaccgtaag ctttttgcgc taaggcaaac agccagggga cggcgaagcc ataaatcccc
     1681 accccagctt cgagcaaacc gtacaataag aagtcgttct tgccgcgatc ggcgatcttg
     1741 ccaaatagat agctaccgat cgccaatccc gccatgaacg cggacagaac ggtggcgata
     1801 gcataggtcg tgttgccaaa gatttgcgtc agcatccggg tccacaccac ctggtaaacc
     1861 agaccggcgg cgccggaaaa gaagaaacaa aacagaccga ccaacagcag ccagtcttgc
     1921 gtcgaacgtt tggaactgac gatcattatt ttccccccgg acgaactcct accgaaacaa
     1981 tccgcagtga tagcaagccc aatgaagact gtaaagggtt gttcgcttta tagctgacga
     2041 ccgcgaacgc gctaaataag acggtaaagt tcatgtaaat agaagcttac gtcagtgctg
     2101 gtttcattga cttttaaaac gatgtcagat aaccccatca agagatcttc cgcgcatcca
     2161 gctttcgctg gtctagaatc tgacagcgat ttaagaccgc tgtgtactta ctgtcagtgt
     2221 caatactgtg ctaactatat agtcagaaag ataaataatt ctggaggaaa gcaatggcta
     2281 aggctgagaa acgagacagc gctccggtcg agtcggtcag agatttacgc agcgccatag
     2341 agtggatgaa aaaaaacggc gatctcatcg aaaccgacaa agaggtcgat cctgacctgg
     2401 agatcaccgg gattcagaaa caattagacg gtggttgccc gatccttttc aataacatta
     2461 agggcaagcc gcaccatcgt tgcatcacca atctttttgg cgatatgaac gtcattggca
     2521 aattattcgg ctggaaagat cacgccgacc gcacaagaaa actggcctac gctctgaccc
     2581 acccgattcc gccggtgatc atcgatcaga aggacgctcc ggtccaagaa gaagtttacg
     2641 agaaacctgc tgacgccaac gaatttgtgg tgccgatccg tcacacggat atcgaacccg
     2701 agttgacggt cggatccggc aacggcttaa tttccggtga gtacttcgat ggtggatcac
     2761 atatcggtta taaccggatg aacttccggt ggggaaatgt gggcactttc caaatctctc
     2821 cgggttcaca tagttggcaa gtcgttacga agtactacaa agataacaag cttgtgccga
     2881 tcacgatgaa ctttggcttg ccgccatcat cgagcctgct ggcaggttcc ggtttcgatt
     2941 acgtcatttt gccgcagggc tgcgatgaaa tcggtatggc gggagccgtg cagggatctc
     3001 cagtccggct ggttaaagcg cgcaccgtcg acgcattggc agttgccgat gccgaagtcg
     3061 ttctcgaagg ctatctcaat ccgagagacc gacgtttcga aaccgccgag tccgaaaagg
     3121 cgggtgtgca aggccgatac catttccacc cggaatgggc cggttatatg ggtaaagcct
     3181 ataaggcgcc gactttccac gtcaccgctg tcaccacgcg caaacgtgaa agcaagccga
     3241 tcatcttccc gctcggcgtg cacactctgg atgaccacaa catcgatacc accgtgcgtg
     3301 aggcggcgat gtttgagcta tgcgaacgga tgcagccggg catcatcatg gacgtcaaca
     3361 ttccctactg catgactgat tggggtggag cgatcatcca agtcaaaaag cgcaataaga
     3421 tcgaggaggg ctggcagcgc aattttatgg cagcgattct tgcgacctcg cagggatcgc
     3481 ggctcgtcat cgccgtcagc gaagacaccg atccgtatga catggacgac gtgatgtggt
     3541 gtctgacaac tcgggtaaat cccaagaccg acatcatcaa tcctttgccg ggtggccgcg
     3601 gccagacctt catgccggcc gagaggatga ctgccgggga gcgggaatgg accgcatcca
     3661 atacgatgtt tgaaggcggc atgggcatcg acgccactgt tcctttcggt tatgaaaacg
     3721 atttcatgcg cccggt
//
LOCUS       DASUTG010000074         7705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_604758, whole genome shotgun sequence.
ACCESSION   DASUTG010000074 DASUTG010000000
VERSION     DASUTG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604758"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF089_01595"
     CDS             complement(<1..180)
                     /locus_tag="VF089_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6172678.1"
                     /translation="MSLSRLASTLSLALGLCLITAIGFAQPAQQYEPQVGQEGKDVVW
                     VPTPQALVDKMLDMAK"
     gene            complement(510..1331)
                     /locus_tag="VF089_01600"
     CDS             complement(510..1331)
                     /locus_tag="VF089_01600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6172679.1"
                     /translation="MKSSFLARILILSVCVSLGSAFGAAHAQPAKSYEPQVGQEGKDV
                     VWVPTPQALVDKMLDMAKVTPNDFVIDLGAGDGRTVITAAKRGAKAHGIEYNPDMVEL
                     AKRNAAKEGVSDRATFVKADLFESDLSQAQVITMFLLSSINMKLRPKILDLKPGTRIV
                     SNTFDMGEWKADETGRADNCNGYCTAYLWIVPAKVDGTWKLPNGELTLKQTFQMISGT
                     LKSGNVVTPVNGKLNGEQITFSAGEGQYTGRVNGNSMEGSLKSGGKWSATRSGKS"
     gene            1686..1949
                     /locus_tag="VF089_01605"
     CDS             1686..1949
                     /locus_tag="VF089_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172680.1"
                     /translation="MEQKRKIEIGSQVYLEEGGDPCGAVRDVRLGNRDEITIYVENSG
                     EFVVSVDAVRAAHDGKVILDRGRLDQSLLDAVAHAHDAEVPGL"
     gene            2242..4935
                     /gene="acnA"
                     /locus_tag="VF089_01610"
     CDS             2242..4935
                     /gene="acnA"
                     /locus_tag="VF089_01610"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX6172681.1"
                     /translation="MVQKYVDSFGTRQVLRVGDQSYDIFRLDLLEKAGFQNIPRLPVS
                     LKVLLENLLRQEDNHHVTKGDIEALANWNPKVKSEKEIAFMPARVLMQDLTGVPAVVD
                     LAAMREAMKRLGGDPKRINPLAPVDLVIDHSVQVDYFGTADALHKNSEIEFKRNVERY
                     AFLRWGQTAFDNFRLIPPDTGICHQVNLEYLAPVVFRAQKDGAHFAYPDTVVGTDSHT
                     TMINGLGVVGWGVGGIEAEAALLGQPIIMLIPEVIGFKLHGKLPEGATATDLVLTVVQ
                     MLRKKGVVEKFVEFYGAGLSSLSLADRATIGNMGPEYGATIGYFPIDDETLRYLELTG
                     RDPHLIDLVESYANEQGMFRTDSSPDPMFTDSLELDLATVVPSLSGPRRPQDRIPLTE
                     SKKSFKEALPSLMKGSDVNKTVTVQNNGDKFQLTHGSVVISAITSCTNTSNPSVMIGA
                     GLLAKKAAEKGLASKPWVKTSLAPGSKVVTEYLKDSGLLPFLEDIGFHLVGYGCTTCI
                     GNSGPLPEPVVNAIQEGDLVAAAVLSGNRNFEGRIHPNVRANYLASPPLVVAYALAGH
                     MDTDLYNEPLGIDSEGKPVFLRDIWPTPEEIQETMRKSVRSEMFTKEYSRATEGDEQW
                     KNMPVPQGELFEWDQQSTYVREAPYFVGMSKTPAELKEIKGARVLALLGDSVTTDHIS
                     PAGSIERNGPAARYLTNNDVLPKDFNQYGARRGNHEVMMRGTFANIRLKNMLAPGTEG
                     GVTVHLPDKKPMSIYDAAMQYQKEGIPLIVVAGKEYGSGSSRDWAAKGPRLLGVKAVI
                     AESYERIHRSNLIGMGIVPLQFKPGENLKSYNLTGFETFEIVGISSGLKLRQTLTVRA
                     TRDDGEVKEFMVICRIDTPAELEYYRHGGILEFVLRQLLQS"
     gene            5117..5923
                     /locus_tag="VF089_01615"
     CDS             5117..5923
                     /locus_tag="VF089_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6172682.1"
                     /translation="MNQPSHIANFTAANPGIMTLQGTNQYIVGKDSVVVIDVALSSDG
                     NMDGIIEQAEAMGAKKIEKILLTHIHVDHCGGALALKKRTGAKLGIHRSRAGYLGGED
                     FQYDDQDRIAFGGGELQVLHTPGHESGHCCFYESGDKVLFSGDNILGYGTAVIHPPDG
                     NMTDYLQSLERLLELSIDLILPGHGPLVGKPQAKIREYLRHRMMREKQVIDALRSGHN
                     TIGDITQSIYVDVSAGLRRVAEFSVQAHLEKLIRDSRVKREDARYYMLGD"
     gene            complement(6153..6854)
                     /locus_tag="VF089_01620"
     CDS             complement(6153..6854)
                     /locus_tag="VF089_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6172683.1"
                     /translation="MSILTILPGVDNLKPFSDLKLLHLLVTVIGLAACSLPPSRIPPP
                     VTASRASQTGIASWYGPGFHGKATASGVIYNQHELTAAHQTLPLGTRVLVTNLDNGRS
                     IEVLINDRGPFAKGRIIDLSYAAAETLGMIGPGTIPVRIEVVDSSTEKISVIKDSLDY
                     TLQLGSFSVLENARQLRDLVASQFSEVAIVPLNTSTSTFYRVQLGKFSTRSSAEEQAR
                     RLTQAGYRVVILEKW"
     gene            complement(6917..7483)
                     /locus_tag="VF089_01625"
     CDS             complement(6917..7483)
                     /locus_tag="VF089_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX6172684.1"
                     /translation="MSFYISEIAAFAAFFVYGSFFEWTLHKYLMHQPRWNYPFRAHAL
                     VHHGLFRTGGDYFLSDKKYLKKVRFAWWNAPFIIALHVPAILLIQNLVGAPILAGACA
                     AMAAYYALYEYLHYCMHIPTGRRLEQTRWFRWLDAHHHMHHKRHLNNLNVVLPLADAV
                     FGSLISAKERIPAPDRQQGAYKMEALLR"
BASE COUNT         1839 a         1933 c         2145 g         1788 t
ORIGIN      
        1 ttttgccata tcgagcatct tatcgaccag agcttgaggc gttggcaccc agaccacatc
       61 cttgccttct tggccgacct ggggttcgta ttgctgtgcc ggctgggcaa atccaattgc
      121 ggtaatcaga cacaggccca gcgccaatga taaagtgcta gctaagcgcg ataaactcat
      181 tttaatcctc cccggctgat gattggcgat acttgtcgta ccgcccctca atagagcaga
      241 caaaaaccac catccatatc ttagcgctgc ctaattgtcc agaaacgagg agccttataa
      301 cgctagcgct cccaggcgct gccaataagt tcgctttcgt atcaattggc tgaaagccgt
      361 agagaaacca aggcccaagt caaaagactc gggcctcaat ctttgtgagc tcgtgagacg
      421 acggcttaag cggctacacg ccggaagcaa caaagcagaa tgagtgcttg aaggcaacgt
      481 taggaattga tctaccgaga gcggtcgttt cacgatttgc cgctgcgcgt cgcgctccac
      541 ttgccgccgg attttaggga tccctccatg gagttgccgt ttacccgccc ggtgtattgt
      601 ccctcaccgg cactgaaggt aatctgctcg ccgtttagtt tgccgttaac cggtgtgacg
      661 acgttcccgg attttagagt gccgctgatc atttgaaagg tttgtttcag tgtcaactcc
      721 ccattgggaa gcttccacgt gccgtctacc ttggccggaa cgatccacag gtaagccgtg
      781 cagtaaccgt tgcagttatc agccctaccg gtttcgtccg ccttccattc tcccatatca
      841 aaggtattcg agacgatgcg cgttcccggt ttgagatcaa gaatcttcgg gcgcagtttc
      901 atattgatgc tcgacaggag gaacatggtg atcacctggg cctgcgataa gtcgctttcg
      961 aacaagtcgg ccttaacgaa cgtagctcga tcactgacgc cttcttttgc ggcattgcgc
     1021 ttggcgagct cgaccatgtc cggattgtat tcgatgccgt gcgctttggc gccgcgttta
     1081 gccgccgtga tgacggtgcg gccatcgccc gcgcccagat cgatgacgaa gtcattgggg
     1141 gtgacttttg ccatgtcgag catcttatcg acgagagctt gaggcgttgg cacccagacc
     1201 acgtccttgc cttcttggcc gacctggggt tcatagcttt ttgccggttg cgcgtgggct
     1261 gctccaaaag cgctgcccag ggatacgcaa acagacaata ttaatattcg cgcgagaaag
     1321 gatgacttca taagtcctcc aataaattat tggctgagca tagcaggttc aaacagagga
     1381 gtggagagct tatgaactga tgccccttaa cgagcggcaa tgtcttgacg gcgcaacaac
     1441 tcgataacgt aacgtcgtcg atacggcaca atccaccatc ggttaacgaa gaaacctgag
     1501 ccggggttca agaagttttt aattttacaa tggtatggag gttgcaaata gctccgtaga
     1561 cagaaggaga atacgttcgg caaagttcaa agctgccgac catcattgaa cctacctatt
     1621 gcgcggcggg ataacctccg tcggcagagg ggtcttcgga ttaagcgcaa aacaaggatg
     1681 gtgttatgga gcagaaaaga aaaatagaga tcggctcgca agtatatctg gaagagggcg
     1741 gcgatccttg cggcgctgtg cgcgatgtgc ggctgggaaa tcgcgatgag atcacgattt
     1801 atgttgagaa ttcgggagag tttgttgttt ccgtggacgc cgttcgcgcg gcgcacgacg
     1861 gcaaagttat tctcgaccga gggcgattgg atcagagcct gctcgatgca gtagctcacg
     1921 cacatgacgc cgaggtcccg ggcctttaat gcgctctccg gcggcttgac cgaccgatcg
     1981 aaaccttaac gttaatcgtc cgcaacgcgc ctcaaatcgt gaccgtacga cggtcacgtg
     2041 caccttcact ccgcttctgt cgtggcgctg ctcccaccgt ataagaagtg tcttccgtta
     2101 atccatctgc tgatgcattc ggcttttcgg tatcctagag gcgcctcaag gccaatgatt
     2161 cctgttgctt tatcctgtta ggctagctac aaaatagacg actcacctaa ggttccgtta
     2221 tacttataga aggagcggtc aatggtacag aagtatgtgg acagcttcgg cacgcgccag
     2281 gtgctgagag tgggcgatca gagctatgat attttccggc tcgatttgct cgaaaaggcg
     2341 gggtttcaga atattccccg tctgcctgtt tctctaaagg tcttgctcga aaacctatta
     2401 aggcaggaag acaaccatca cgtcaccaaa ggcgacattg aagccctcgc aaattggaac
     2461 cccaaggtta aatcggaaaa agagattgcc ttcatgcccg cgcgggtgct gatgcaggat
     2521 ctcactggag ttcctgccgt agtcgatctt gcggcgatgc gcgaggcgat gaagcgacta
     2581 ggcggtgatc cgaagagaat caacccccta gctcctgtcg atctggtgat cgatcattcg
     2641 gtccaagtcg attacttcgg cacggcggat gcgctgcaca agaattcgga aattgagttc
     2701 aagcgaaatg tggagcgtta tgctttccta cgctggggcc aaaccgcctt cgacaatttc
     2761 cggctgatcc caccggatac cggaatctgc catcaggtaa acctggaata ccttgcgccg
     2821 gtggttttcc gcgcgcaaaa agacggagcg cacttcgcgt accctgatac cgttgtcggc
     2881 accgactcgc acacgaccat gatcaatggt ttgggggtgg tcggctgggg agtgggtggc
     2941 attgaagcgg aagcggcgct gcttgggcag ccgatcatca tgctgattcc tgaggtaatc
     3001 ggctttaagc tgcacggcaa acttccggaa ggagcaactg caacggatct ggtcttaacg
     3061 gttgtgcaga tgctcaggaa aaagggagtg gttgagaagt tcgtcgagtt ttatggcgca
     3121 ggtttgtcga gtctgagttt ggccgatcgc gcgaccatcg gcaacatggg gcctgaatac
     3181 ggcgccacga ttgggtattt tccaattgac gacgagacct tgcgctactt ggaactaaca
     3241 ggccgagacc cgcatctgat cgacttggtg gaaagttatg ccaacgagca aggcatgttc
     3301 cgcaccgatt cttcgcccga ccctatgttc acggattcac ttgagcttga tttggcaacc
     3361 gtggtaccca gcttgtccgg tccgcgccgg ccgcaagacc gcatacctct aactgaaagc
     3421 aaaaaatcat tcaaagaagc gctaccatcg ttaatgaagg gctcggatgt caacaagacg
     3481 gtgactgtgc agaataatgg cgataagttc cagctcacgc atggctcggt tgtcatctcc
     3541 gcgatcacaa gttgcaccaa cacatccaat ccgtcggtca tgatcggggc cggattgttg
     3601 gctaagaagg ccgcagagaa gggtctggcg agtaaacctt gggttaaaac cagtctggcg
     3661 ccgggatcca aagtggtgac cgagtatctc aaggacagcg ggcttctgcc gttcttagag
     3721 gatatcggct ttcatctggt tggttatggc tgcaccacct gtatcggcaa tagcggtcca
     3781 ctgcccgagc cagtggtcaa cgcgattcag gaaggagact tggttgccgc cgcggtgttg
     3841 tccggtaacc gaaactttga aggacgcatt catcctaacg tgcgggcaaa ttatctcgct
     3901 tcaccgcctc tggtggttgc ctatgcgctg gccggtcata tggatacgga tctctataat
     3961 gagcctctgg gaattgattc agagggcaag ccggttttcc tgcgagacat ttggccgacg
     4021 ccggaggaaa ttcaagagac gatgcgcaag tcggtgcgct ctgaaatgtt cacaaaggaa
     4081 tatagccggg ccaccgaagg cgatgagcaa tggaagaaca tgccggtgcc acagggtgag
     4141 ctgtttgaat gggatcagca gtcaacgtac gtacgtgaag cgccatactt cgttggaatg
     4201 tcgaagactc cggccgagct aaaggagatc aaaggagcgc gtgtattggc gctgcttggt
     4261 gattcggtta ccaccgacca tatttcgccg gctggttcaa tcgagagaaa cggcccggcg
     4321 gcgcgctacc ttaccaataa tgatgttttg cccaaggact tcaaccagta tggcgcgcgt
     4381 cgcggtaatc atgaagtcat gatgcgcgga acgtttgcaa acattcgttt gaaaaacatg
     4441 cttgcgccgg gcactgaagg cggcgtgacg gttcatttgc cggacaagaa gccaatgtca
     4501 atctacgacg ccgcgatgca atatcaaaag gaagggatcc ccctcatcgt cgtcgctgga
     4561 aaggagtacg gctccggttc gtcgcgagac tgggcggcca agggtccgcg cttgttaggc
     4621 gtgaaagcgg ttatcgccga gagctatgaa cgtattcacc gcagtaactt gatcggcatg
     4681 gggatcgtgc cgctgcagtt taagcctggt gaaaatttga aatcttacaa tctcaccggt
     4741 ttcgaaactt tcgaaatcgt cggcatctcg tcaggcctca agttgagaca aactcttacg
     4801 gtcagagcga ctagagatga tggcgaagtt aaagagttca tggttatttg ccgcatcgat
     4861 accccggccg agcttgaata ttaccgtcac ggcggaattt tagagttcgt gctcaggcaa
     4921 ctgttacaaa gctgatcacc cgaaaagggg agactcgcac aatttagaca gggttagctt
     4981 gaccgaaccg ggattcgcaa acccacccat gaatcggaga caagcactca ggttaatggg
     5041 acctggttcg ctggcgcttc ttttcggcaa ttcgcgacca ttcccgcgac atatctacgc
     5101 cgctgaaagg ccgataatga atcaaccatc tcacatcgca aactttaccg cggctaatcc
     5161 aggcatcatg actttgcagg ggaccaacca atatattgtc ggcaaggaca gcgtggtggt
     5221 catcgacgtg gcgctgagct cggatggaaa catggatggg atcatagaac aggccgaagc
     5281 catgggcgct aaaaaaatcg aaaagatcct tctgacgcac atccatgttg atcattgtgg
     5341 cggggcgctt gcgctgaaga agcggaccgg cgccaagctg ggtattcacc gttcgcgcgc
     5401 aggctatctg ggcggggagg actttcagta cgacgatcag gacaggattg ccttcggtgg
     5461 cggtgagctc caagtattgc acactccagg tcatgaatcg ggtcactgct gcttctatga
     5521 gtcaggcgat aaggtgctct tcagcggtga caatatcttg ggatacggaa ctgccgtgat
     5581 tcacccgccg gacggtaata tgaccgatta cttgcaatcc ttggagcggc tcttggagct
     5641 tagtatcgat ttgattctgc ctggtcacgg gcctttggtc ggtaaaccgc aggccaagat
     5701 ccgggaatac ctgcggcatc ggatgatgcg cgagaagcag gtgatcgatg ccttgcgcag
     5761 cggacataat accatcggcg atatcaccca atcgatctat gtggacgtgt ccgccgggct
     5821 gcgacgggtg gcggaatttt cggttcaggc gcacctcgag aagcttatcc gggacagccg
     5881 cgtcaagaga gaagatgccc gctactatat gcttggcgat tgatatatcg cgttgtgccg
     5941 aatcaaaggc atcggttcgg tcccggtatt tgcctaaaac cgaaccgcgc gttcatcgat
     6001 gaactaacac aaacattgaa cggaacttta gcgtgagcag gggaacggcc ttgtcgctga
     6061 acagttgttg gcttcgctag gaaaaggcaa caatcaaacg agactagaag gcgctgcgct
     6121 cgttatcgca gctggttgtg gctgaaattg ctctaccact tctcgagaat gacaacgcga
     6181 tatccggcct gtgtcagccg tcgcgcctgt tcttcggcac tggagcgagt agaaaatttc
     6241 ccgagttgga cacgataaaa cgtgctcgta ctggtgttca gcggcacaat ggcgacctcg
     6301 gaaaattgtg atgcgaccag gtcgcgcagc tggcgagcat tctcgagtac tgaaaatgag
     6361 cctagctgaa gagtgtagtc cagtgaatcc ttgatcacgg atatcttctc agtgctgctg
     6421 tcgacaactt caatccgcac cgggatagtt ccgggaccga tcatgccgag ggtttccgct
     6481 gccgcatagg acaggtctat gattcttccc ttcgcaaatg ggccgcgatc gttgatcaaa
     6541 acttcaatgg acctgccatt atctaagttg gttaccagca ctcgagttcc gagcggcaat
     6601 gtttgatgcg ccgctgtaag ctcgtgttgg ttgtagataa ccccgctggc ggtagccttg
     6661 ccgtggaaac cgggcccata ccatgaggct atgccggttt ggctggcgcg cgacgcggtg
     6721 acaggaggag ggattctcga gggcggtaag gaacacgccg ccaggccgat gactgttacc
     6781 aataagtgaa ggagtttcaa atcggagaac ggcttcaaat tatctactcc aggcagaatt
     6841 gtcaggatcg acatttatca gaccgctggt gccaaagcac attgaaaaat ggagtcactg
     6901 tcgaatcccc ttaggattaa cgtagcagcg cttccatctt gtaggcaccc tgctgccggt
     6961 ccggagcagg aattctttct tttgcgctta tcaaactacc aaacacggcg tctgccaatg
     7021 gtaaaacaac attgagattg tttaggtgcc gtttgtgatg catatgatga tgcgcgtcga
     7081 gccaacgaaa ccagcgagtt tgctcaagac gccgtccggt gggaatgtgc atgcagtagt
     7141 gcaaatactc gtaaagcgca taataagccg ccatggcggc gcaggcgccg gcgagtatag
     7201 gcgcgcctac aagattctgt ataagaagta ttgcaggaac gtgaagcgcg atgatgaacg
     7261 gtgcgttcca ccaggcgaag cgaacctttt tgagatattt cttatctgaa agaaagtaat
     7321 cgcctcctgt acggaagaga ccatggtgga ccaaggcatg cgcgcgaaat ggatagttcc
     7381 agcgcggctg atgcatgagg tatttgtgta aagtccattc gaaaaagctt ccgtacacga
     7441 aaaaggctgc aaacgcggcg atttcactta tgtaaaatga catgatttgt tacgctccct
     7501 gtgtcacgtt gagtaaacgg ctctcgaaga ttttattttg gaaggcttat gatgccagtt
     7561 ggcacggcgg aatgcagatt ctctaataga tgagttcctg ttgagctctt gaaaaacgtt
     7621 ttctacacta cgtcgttttg tgcgaatggt caattgcgat gcttcacggt gcattatcaa
     7681 tgactctcgc cgtttacgca gcgca
//
LOCUS       DASUTG010000075         8169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_901315, whole genome shotgun sequence.
ACCESSION   DASUTG010000075 DASUTG010000000
VERSION     DASUTG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901315"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="VF089_01630"
     CDS             <1..1620
                     /locus_tag="VF089_01630"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172685.1"
                     /translation="FIAALDAHDKLYRIHREINKDSELQPLVRWQFRGLAEEARRGFL
                     FDNVTDGKGRKYNCRVLVGGLAGSEAIYCLGLKCQPDEVADRWLYAMDHPIDPIIVGS
                     GPCQEEVHTGNDLLSQGGLLEFAVPMSTPGFDNGPYITAGHWITKDPETGRRNVGNYR
                     GLIKGPALSGLMSGTPQDLSSHWEKCRRLGKPLEVAIVVGTVPAVSYCATQKVPPDVD
                     VIALAGGLTGAPVKLVKCKTVDLEVPATAEIVLEGIIPTNYMEEEGPYGESMGYVDPR
                     TLSTMFELKCVTHRKDPIWVSIISQVTPSESSKIKAMGMSTLVKRFIVSKGFDCVREV
                     HMIEPLVNLRPYVAVSLKKRNDEDPWGVMQAVLDYGDRVGKMIVAVDEDINVKDPVAV
                     TWAITHRSQPHKDIKVVGDRPFGATPIGMVATHPSSRYDNCESSLLIDATRKADFPPL
                     SLPKKEFMVRAREIWEELGLPKLEPQEPWHGYLMGLWPEDLEEEAELAAKSEHEKVWE
                     KLRQTRVQVGEGETLKSMRAKWGKSHTGRSE"
     gene            1623..2186
                     /locus_tag="VF089_01635"
     CDS             1623..2186
                     /locus_tag="VF089_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6172686.1"
                     /translation="MKKNVTFTVNGEEHEVEIESNRLLLQVLRDTLGLTGTKEGCSIG
                     VCGACSVIVDGRVVSSCLTLAASCAGRDIQTIEGLAKDGKLHPLQQAFIEYGGFQCGI
                     CTPGQIMAAKSLLDENPTPTEDEVKEWMSGNLCRCTGYYKILEAVMAVVNNKIDPDFA
                     FRKREQTAKIQSLDVYKAVAKTPHSQK"
     gene            2307..4604
                     /locus_tag="VF089_01640"
     CDS             2307..4604
                     /locus_tag="VF089_01640"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6172687.1"
                     /translation="MAKAEKFSVVGRRVQRVEGFDKVTGDSKFIADIFLPNMLVGKVL
                     RSPFPHARIRSIDTRKAEKLRGVRAVVTAEDTIKRPWGAFFADQYILSVGKTRYVGEE
                     VAAVAAIDPDIAEEAIDLIEVEWEPLPGVFDAEEAMQDGAPLVHDDKPNNIAMTMDIE
                     RGNIAQAFAQSDVIVEDTFQSMPQWHCSIETIGSVAEYSPNGKYTIYMNTQTLFNARY
                     RIATALGVPETDVRIIQSAVGGGFGGKSCDDNNALVAAVLSRKAGKPVKLINTREEEF
                     LAGCRPRVFMKITVKLGFKKDGRIRAKHIKVIADNGAYSAKAPAITGVAAMRHDTCYK
                     YSDVKTEAHLVYTNKIPTGAFRGFGNPSAEWAVEQAIDLGAHELGIDPIEIARINAAE
                     TGYVSPHGNRVTSCELKQCLDMAEKMIDWKNQRANKQPNTGLGLACTVHVSGKRHFGD
                     YDGSSATIKLNEDGKALILSGEGECGQGVHTAMCQIAAEELGLPVEDVEISRADTDLT
                     TFCLGAFASRLTYVSGNAVKNAAANVKQQLFQQAAEMLEANPEDLVSNDGQIFVAGTQ
                     GKSVSVADVARARLFRHNGAPIVGSGSFDADSVLPDSTRFGNESGAYNYGVQAAQVRV
                     DPETGQVEILKFVIASDCGTVIYPLGAEGQVEGGLSQGIGYALTEGLQIDEGKPVNPN
                     FSDYRIPSMRDMPPLQHAFAESYEPTGPFGAKGLGELNMDPTAAVISNAIFDAVGVRI
                     KTLPITPEKILRALQEKRAGESAQS"
     gene            4601..5461
                     /locus_tag="VF089_01645"
     CDS             4601..5461
                     /locus_tag="VF089_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6172688.1"
                     /translation="MIRFDYLEPATLSEAFSLLEQHGDNARVIAGGTSLIIWMRQRLL
                     MPKVVIGLGRIPDFDTITFDPQEGLRIGAGARHRDIELSPAVKQHYPLLQETFRKVAQ
                     PRIRNMGTIGGNLAGGDPLTDPGASLIALDAELTLASSNGNRTVSLEDFFVDYYQTAL
                     EPGELLTEIRVPPPQRPGWSHIKFTPRSVEDFATVGVAVTLRVTAGSCEDVRLALNSV
                     ASTIVRAKRAEDVLRGHRLTDNLLREAGEVAASEVDPMDDNRGSADYKRDLVKVLVRR
                     AAEQAVQRLS"
     gene            5493..5966
                     /locus_tag="VF089_01650"
     CDS             5493..5966
                     /locus_tag="VF089_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6172689.1"
                     /translation="MKTTIRVTVNGRLYEEDVEPRILLSHFLRETIGLTGTHVGCVVG
                     ECGACSVLLDDKLIKSCLHFAVQADGCSVTTIEGLAKDGQLSVIQEAFVKHYALQCGY
                     CTPGMVMASHALLRDHPQPTEEEIRKALGGNLCMCTGYVQIVEAVKDAAKGSNHV"
     gene            5959..>8169
                     /locus_tag="VF089_01655"
     CDS             5959..>8169
                     /locus_tag="VF089_01655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6172690.1"
                     /translation="MSDFIGSRTRSKEGPRHVSGRGLYTDDFTLPGMLQAMILRSPHA
                     HAKILSVDPSQALANPNVIAVITPDDIKQNTKPFKPGRYAAGLKRPIDEYAGAVDKVR
                     YVGEPLGAVAARDRGTAEDALELISVEYEPIRPVVDVREAIKPSSATLFDELGSNLAW
                     HGSLQYGDIDGAFKSADRVVKENLKIHRYSSTPLEPFAVIVSYDSASKRLTVWVTAQV
                     PEVIYDGLREALGLQDVRVIIPDVGGGFGQKIHLIRKYVIMVALLSMKTGRPVKWVED
                     RSEHMMAGGHACAQEFEAEAAVKNDGTVLGLRFKEYDDVGGSISTLTIHFTNKLNNLS
                     NTYRTPSIAMEGYAVVTNKCPVIPNRGIGKPGMCYVWERMMDRVAQELNMSPIEVRRK
                     NLIQPSEMPYRTISGNVYDSGDYPGLLSTLLEKIDYDKLREEQKREREKGRLIGIGIV
                     IGVEPGGRNAARDMAIFPEMKEPPGSGGINGATIKLEKNGTIALHLGSPNCGQAHETT
                     TAQVAADVLGTTPDQISTSIPFDSDLSPWGVAAANSGNNFHLYDIGAIHGAAKKLREK
                     VVKLAAHLLNADAAELTIEKGVVQVPGSATKKVTFAELGRVAYNNQHLIPDDMEAGLQ
                     ATYYYTFPHAKPNLVPGADRLVRAQFTFSAGAHAAIIEVDKATGKVEILRYLIVGDNG
                     TVINPDVVNGQVFGSAAHGIAVALGEGFIYSPEGQPLTVTYLDYGKCSTAETPKV"
BASE COUNT         1944 a         2247 c         2340 g         1638 t
ORIGIN      
        1 tttatcgccg cactcgacgc tcacgacaag ctgtaccgaa ttcaccgaga gataaataaa
       61 gacagcgaat tgcagccatt ggtacgctgg cagtttcgcg gcttagccga agaagcgcgc
      121 cgtggctttc ttttcgacaa tgtcacggat gggaaaggac gcaaatacaa ttgccgagtg
      181 ctcgtcggtg gtctcgccgg ttccgaagcg atctactgtc tgggcctgaa gtgccagccg
      241 gatgaagttg ccgatcggtg gctctacgcc atggatcatc cgatcgatcc gatcatcgtc
      301 ggctcaggac cgtgccagga ggaggttcac acagggaacg atcttttgtc ccagggcggt
      361 ttactggagt ttgccgttcc gatgtccacc ccgggattcg acaatggtcc ttatattacc
      421 gccggtcatt ggatcaccaa agatccggaa accggcagga gaaacgtcgg caattatcgc
      481 ggattgatca aagggcctgc gctgagtgga cttatgtcgg gaacccctca ggacttgtct
      541 tcgcattggg agaaatgccg caggctcggt aagcctctgg aggttgccat cgtcgtcggc
      601 acggtccccg ccgtatctta ctgcgcgacc cagaaggtac cgccggacgt cgatgtaatc
      661 gcgttggccg ggggtctcac aggcgcgccg gtgaagttag tcaaatgcaa gacggtcgac
      721 ctggaggtgc ctgccaccgc cgaaattgtt ttggaaggta tcattccgac caactatatg
      781 gaggaagagg gtccttacgg cgagtcgatg ggttatgtcg atcctagaac cttgagcacg
      841 atgttcgagt tgaagtgcgt tacccaccgc aaagacccga tatgggtgtc gatcatcagc
      901 caggtcactc ccagcgagag ttccaagatt aaagctatgg ggatgtcgac actggttaaa
      961 cgattcatcg tcagtaaagg cttcgattgc gtccgcgagg ttcacatgat cgagccgctg
     1021 gtgaatctgc ggccttatgt tgccgtcagt ttgaagaaaa ggaacgacga agatccttgg
     1081 ggcgtcatgc aggcggtcct ggactatggc gatcgtgtcg gaaaaatgat cgtcgcagtc
     1141 gacgaggaca tcaacgtcaa agatccagtt gccgtcactt gggccatcac ccatcgctcc
     1201 caacctcaca aggacatcaa ggttgtcggt gaccggccgt tcggcgccac ccccatcggc
     1261 atggtcgcaa cgcatccttc gagccgttac gacaattgcg aatcgtcgtt gctgatcgac
     1321 gccacccgta aggcagactt tcctccgcta tcactgccga agaaggagtt catggtccgc
     1381 gcccgcgaga tttgggaaga actcggcctg ccgaaactcg aaccgcaaga accttggcat
     1441 ggctatctga tgggtttatg gccagaagat ctagaggaag aagccgagtt ggcagcgaaa
     1501 agcgaacacg aaaaagtctg ggaaaagctg cgccaaactc gggttcaggt cggcgaaggc
     1561 gagacgttga aatcaatgcg ggctaaatgg ggtaaatccc acactggacg ctcggaatag
     1621 ccatgaagaa gaacgtcact tttaccgtca atggtgagga acacgaggtt gagattgagt
     1681 cgaaccgcct cctgctccaa gtcctcaggg ataccctcgg cttaaccgga acgaaggaag
     1741 gctgctccat cggtgtctgc ggtgcatgta gtgtcatcgt cgacggcagg gtcgtgagct
     1801 catgcctgac gttggcagcc agctgcgccg gaagagatat tcaaaccatc gaaggtctcg
     1861 ccaaggacgg caagctccat ccactgcagc aggcgttcat cgagtacggc ggctttcaat
     1921 gcggcatctg cactcccgga cagatcatgg cggcaaagtc cctattggac gaaaacccca
     1981 cgccgaccga agatgaagtc aaggaatgga tgtcgggaaa tctctgccgc tgcaccggct
     2041 actataaaat tctcgaagca gtgatggcgg tggtcaataa caagatcgat cccgatttcg
     2101 catttcgcaa gcgcgagcag accgccaaga tccaaagcct tgacgtttac aaggcggtcg
     2161 caaagacgcc tcattcacaa aaatgaggga ggcgccctga gtgcagcgaa gggcctcaat
     2221 tccaagtgaa aggattcgcc gtgagcgaag cgcggcataa ggatcgatca aacgtcgcac
     2281 gaaaaactaa aaagagatcg taacatatgg caaaagcaga aaagttctcc gtcgttggtc
     2341 gacgcgttca gagagtcgaa ggtttcgaca aagtcaccgg tgattcgaaa ttcatcgccg
     2401 atatttttct tcccaatatg ctggtcggga aagtgctgcg gagtccgttt ccgcatgcgc
     2461 gaatccgcag catcgatacc cgcaaagcgg aaaaactccg cggcgtgcgc gccgtggtca
     2521 cggccgaaga taccatcaaa cgaccgtggg gtgctttttt cgcggaccag tatattcttt
     2581 ccgtcggaaa aacccgttac gtcggcgaag aagtcgccgc agtcgcggcc atagatcccg
     2641 acatcgccga agaggctatc gatctgatcg aggtcgaatg ggaaccgctg cccggggtct
     2701 tcgacgccga agaagcgatg caagacggcg caccgctggt gcatgatgac aagcccaata
     2761 atattgcaat gacgatggac atcgagcgcg gcaacatcgc ccaagcgttc gcccagtctg
     2821 atgtcatcgt cgaggatacg ttccagagca tgccgcaatg gcactgctcg atcgaaacca
     2881 tcggtagcgt tgcggaatat tcgcctaacg gcaaatacac gatctacatg aacactcaaa
     2941 ccctatttaa cgcgcgttac cgaattgcca ccgcgctggg cgtgccggag accgatgtcc
     3001 ggattatcca gagcgcggtc ggcggcggct tcggcggcaa gtcctgcgac gacaacaatg
     3061 cattggtggc cgcggttcta tcgcgcaaag ccggcaaacc ggtaaagctg atcaacacgc
     3121 gtgaggaaga attcctggcc ggctgccgtc cgcgggtttt catgaagatc acagttaagc
     3181 tgggcttcaa aaaggacggt cgcattcgag ccaagcatat caaggtgatc gccgataacg
     3241 gcgcctattc cgcgaaagcg ccggcaatca ccggagtcgc ggcgatgcgc catgacacgt
     3301 gttataaata ttcggacgtc aaaaccgaag ctcatctggt ctacaccaac aagattccaa
     3361 ccggcgcgtt ccgcggattc ggcaatccgt cagccgagtg ggcggtcgag caggcgatcg
     3421 atctgggcgc tcatgagctt ggcatcgatc cgatcgaaat cgcccgtatc aatgcggccg
     3481 aaaccggcta tgtgtcgcct catggcaacc gagtcaccag ctgcgagttg aagcagtgtc
     3541 tcgatatggc ggagaagatg atcgattgga aaaaccagcg ggccaacaag caaccaaata
     3601 ccggcttggg gctggcatgc accgtccacg tgagcggcaa gcgtcacttc ggcgactacg
     3661 atggcagttc cgcgacgatc aagctcaacg aagacggtaa agcgttgatc ctcagcggcg
     3721 aaggcgaatg cggccaggga gttcataccg cgatgtgcca gattgccgct gaagaattgg
     3781 gcctgccggt tgaagacgta gagatctcgc gcgccgacac tgatttgacg acattctgcc
     3841 taggcgcttt cgctagccgg ctcacctacg tcagcggcaa tgcggtaaag aacgccgccg
     3901 ctaacgtgaa gcagcaactt tttcaacaag cggctgaaat gcttgaagcc aatcccgaag
     3961 atctcgtctc caatgatggt cagatctttg tggcgggcac ccaaggcaaa tcagtctccg
     4021 tcgccgatgt cgctcgagca cgcctgttcc gccacaacgg cgcgccgatc gtcggctccg
     4081 gcagcttcga tgccgattcc gttttgccgg acagcacacg tttcggcaac gaatcgggcg
     4141 cgtacaatta cggcgttcag gcggctcagg ttcgggtcga tccggaaacc ggacaggtcg
     4201 agatattaaa gttcgtcatc gcatccgact gcggcacggt catttatcct cttggcgccg
     4261 aaggccaagt ggaaggcgga ttgtcgcagg ggatcggcta tgccctcacg gaaggtttgc
     4321 aaatcgatga aggcaaaccg gtcaatccaa acttttctga ctaccgcatt ccatcgatgc
     4381 gagacatgcc gccgctacag catgcatttg cggagtccta cgagccgacc gggccttttg
     4441 gcgccaaggg attaggggag ttgaacatgg atccgacagc ggcggtcatc agcaatgcaa
     4501 ttttcgacgc cgtcggagtg cgcatcaaga cgttgccgat cacgccggag aagatccttc
     4561 gggccttgca agaaaaacgc gccggagaaa gcgcccagtc atgatcaggt tcgactattt
     4621 agagccggct actctatccg aggctttttc actcctagaa caacacggag ataatgcccg
     4681 ggtgattgcc ggcggcactt ccctgatcat ctggatgcgc caacgtctgc tgatgcccaa
     4741 ggtggtaatc ggcctcgggc ggattcctga tttcgacacg attacgttcg acccacaaga
     4801 gggcctgcgg atcggcgccg gcgcgcgcca ccgtgatatc gagctttcac cggcggtgaa
     4861 gcagcactat cccctgcttc aagagacctt ccggaaagtc gcgcagccaa gaatccgtaa
     4921 tatgggaacc atcggcggca atttggccgg tggcgatcca ttgacggacc ctggcgccag
     4981 tttgatcgct ctggatgcgg aactcacgtt ggcaagcagc aatggaaaca gaaccgtatc
     5041 tttagaggat ttctttgtcg actactacca gactgcgcta gagccaggag aactattgac
     5101 cgaaatccgc gttccacccc cccaacggcc cggctggtct cacatcaagt ttaccccgcg
     5161 cagcgtagaa gattttgcaa ctgtcggcgt agccgtgacg ttgcgcgtca cggccggctc
     5221 ctgcgaagat gtgcggctgg cgctcaactc agtcgcttcc actatcgtaa gagccaaacg
     5281 cgccgaagat gtcttacgcg gacaccgact tactgacaat cttctccgag aagccggcga
     5341 ggttgccgca agcgaggtcg atccgatgga cgataatcgc ggctcggccg attataagcg
     5401 cgatctggtg aaagtgctgg tgcgccgcgc cgctgaacaa gccgttcagc gccttagttg
     5461 acccgttttc cagcaaacgt tagttttacc ctatgaaaac cacgatacgc gtcactgtta
     5521 atggacggtt gtatgaagaa gatgtcgagc cgcgcatttt gctatcgcac ttcctgcgcg
     5581 aaaccatcgg cttaaccggc acccacgtgg gctgcgtcgt cggcgaatgc ggcgcatgct
     5641 cggttcttct cgacgacaag ctgattaagt cttgtcttca cttcgcggtg caggcggacg
     5701 gctgcagtgt gaccacaatc gaggggctgg ccaaagatgg tcagctaagc gttattcaag
     5761 aagcgttcgt taagcattac gctttacaat gcggctactg caccccgggg atggtaatgg
     5821 caagccatgc cctcttgcgc gatcatcccc agcctaccga agaggaaatt cgcaaagcgc
     5881 tcggcggcaa tctctgcatg tgcaccggct atgtgcagat agtcgaagct gtaaaggacg
     5941 cggcaaaagg atccaaccat gtctgatttt atcggcagcc gcacacgcag caaagaaggg
     6001 ccgcgacatg tcagcgggcg agggctttat accgatgact tcacgttgcc cggcatgctg
     6061 caggcgatga ttttgcgaag tccgcacgct cacgccaaaa ttctgtccgt cgatccgtcg
     6121 caagctttgg ccaatccaaa cgtaatagca gtgatcaccc cggacgatat caagcaaaac
     6181 accaaaccgt ttaagccggg tcgctatgct gccggcctga aacgaccgat cgacgagtat
     6241 gccggcgccg tcgacaaagt gcgctatgtc ggcgagcctc tcggcgcagt tgccgctcgc
     6301 gaccggggca ccgcggaaga tgctctggag ctcatttcgg tcgaatacga gccaatccgg
     6361 ccggtggttg atgtgcgcga agcgatcaag ccttcctccg caaccctgtt cgacgaactg
     6421 ggcagcaatc tggcgtggca tggctcgctc caatacggcg atatcgatgg cgcattcaag
     6481 tccgccgacc gggtagtcaa ggaaaatcta aaaattcacc gttacagctc gacaccgctg
     6541 gagccattcg cggtcatcgt atcctacgat tctgccagta aacggctgac ggtctgggtt
     6601 acggcacagg tgccggaagt gatttacgat ggtttgcgtg aggcgctcgg gctgcaagac
     6661 gtgcgcgtga tcattccgga tgtcggcggc ggtttcggac agaaaattca tttgatacgc
     6721 aagtacgtca tcatggtcgc attgctatcg atgaaaaccg gcaggccggt gaagtgggtg
     6781 gaagatcgta gcgaacacat gatggctggg ggacacgcgt gcgcccagga atttgaggcc
     6841 gaggctgcag tcaaaaatga cggcaccgtg ctgggcctgc gctttaaaga atacgacgat
     6901 gtcggcggct caatcagcac cttgacgatt cattttacca ataagttaaa caatttatcg
     6961 aacacttacc gcacccccag tatcgccatg gaaggttatg cggtggtcac caacaaatgc
     7021 ccggtgattc caaaccgggg tatcggtaag cccggcatgt gttacgtctg ggagcggatg
     7081 atggatcgtg tcgcccagga actgaacatg agtccgatcg aggtgcgacg taaaaatttg
     7141 attcaaccga gtgagatgcc gtaccggacc atcagcggca acgtctacga cagcggcgat
     7201 tatcccggct tgttgtcaac tctcctggaa aaaattgact acgataagct gcgtgaagag
     7261 caaaaacgcg agcgcgaaaa aggccgctta atcggcatcg gtatcgtcat cggtgtcgaa
     7321 ccgggtggcc ggaacgcggc ccgagacatg gcgatctttc ctgagatgaa agagccgccg
     7381 ggatcgggcg gtatcaatgg cgccaccatc aagcttgaaa agaacggcac tattgcgttg
     7441 catctgggat cgcctaattg cggccaagca cacgagacga cgacagccca agttgccgcg
     7501 gatgtcttag ggaccactcc tgaccagatc agcacgagta ttcctttcga tagcgatctg
     7561 tcgccttggg gcgtggccgc ggccaacagc ggcaacaatt ttcacctcta cgacatcggc
     7621 gcgatccacg gcgcggcaaa aaagctgcga gagaaggtgg tgaagttagc cgcccacctg
     7681 cttaacgcag atgccgcgga attgactatc gaaaagggtg ttgtccaggt gccgggttcg
     7741 gccaccaaga aagtcacatt cgccgagctc ggacgcgtcg cctacaacaa tcagcatcta
     7801 attcccgacg acatggaagc gggactacaa gcgacctact attacacgtt ccctcacgcg
     7861 aagccgaacc ttgttcccgg cgcggaccgt ttagtgcgcg cgcagtttac tttttcggcg
     7921 ggcgctcacg cagccatcat agaagtcgat aaagcaacag gcaaagttga aatcctgcgc
     7981 tatctcatcg ttggcgataa cggcacagtg attaaccccg acgtggtgaa cggtcaggtt
     8041 ttcggctctg cggcacatgg catcgccgtt gcgctggggg aaggttttat ctacagcccg
     8101 gaaggccaac cgcttacggt aacctatttg gattacggca aatgctccac ggcggagact
     8161 ccaaaggtc
//
LOCUS       DASUTG010000076         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_12152, whole genome shotgun sequence.
ACCESSION   DASUTG010000076 DASUTG010000000
VERSION     DASUTG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12152"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF089_01660"
     CDS             complement(<1..573)
                     /locus_tag="VF089_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172691.1"
                     /translation="MANLVGCFAMSHGPQLMLEPNHWNLLRMRESEKLPERAELESET
                     DEVKWQKWHHCMDAIAQLRQKLEALRPDVAIVIGDDQHENILDDNTPPFTIFIGDEAE
                     ATTSLRYLNQARSDNRTRYEIDSELAKRLLADLMGNGFDPAYSKMTRYDGGLGHAFAR
                     VLKFLLPGASCRVVPIMVNTYFPPAPSPQRC"
     gene            981..2201
                     /locus_tag="VF089_01665"
     CDS             981..2201
                     /locus_tag="VF089_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6172692.1"
                     /translation="MRRYWQPIALSEELPPGGAPLRVNVLGEELVLFRDDHGRPGLLG
                     LHCSHRGTDLSYGRIENGGLRCIYHGWLYDVCGRVIEQPGEPGGGANRDQIRHRAHPC
                     RETGGVILTYMGPGEPSLLPDYEFLSVPEEQRTVTKILYSCNYLQGNEGNIDPVHLSF
                     LHQFLGDAPAPLRRVVPGTETTDNTLLRKDIAPTIEVELTDFGLRICSLREAGQNKTY
                     LRVTNFVMPNLAAFGGSTVGEGYSVHWHVPIDDTSHWKYVFMFSREKALPPELINKSR
                     VELGADFRLSRTGANRYQQDRESMKTKTFTGIGYNFQAHDAFATESQGVIQDRTAEHL
                     VSSDKAIVAARKLLLNAINDVKQGRDPQHVVRDPASNRFSHLQVFSEVIDASVDWKQH
                     TKERIATIERIRSR"
     gene            2250..>2488
                     /locus_tag="VF089_01670"
     CDS             2250..>2488
                     /locus_tag="VF089_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172693.1"
                     /translation="MTWKNILGLLLFLCILAPAAAAQERYNIAYAGFAGFQTPVWATK
                     DLGLLAKYGIDGEVVLLPGSTRQIQGLVGNSVDFA"
BASE COUNT          553 a          648 c          710 g          577 t
ORIGIN      
        1 gcagcgttga ggcgagggag ccggtggaaa ataggtgttg accatgatcg gaacaacccg
       61 gcagcttgca ccgggtagga gaaacttgag cactcgcgcg aaggcatggc ccaagccgcc
      121 gtcatagcgg gtcatctttg aataggcggg atcaaatccg ttgcccatca gatcggctaa
      181 cagacgcttg gctaattcgc tatcgatttc ataacgggtc cgattatccg atctggcttg
      241 attgaggtac ctcaggctgg tggttgcttc cgcttcatcg ccgataaaaa tcgtaaatgg
      301 cggagtattg tcatcgagga tgttttcgtg ctgatcatcg ccgataacga tagcgacgtc
      361 cgggcgcaaa gcttctaact tctgccggag ctgggcgatg gcatccatgc aatgatgcca
      421 cttctgccat ttaacctcgt cagtttcgct ttcgagctcg gctctttccg gcagcttctc
      481 ggattctcgc atgcgcaaca ggttccaatg attgggctcg agcatcaact gcggcccgtg
      541 tgacatcgca aaacaaccga cgagattagc catggctttg actttctttg gcgaacgtct
      601 gtgctttact cggctccaac ctactatagc aaacgttctc ggttcaaggt gaacgggact
      661 gttcaaccgt tcaaggttca acagttcgag gtcagaggat aggatcggat gcacctgggc
      721 gtgtcgacca ggatgcctga aacgatcatc cccggggata cggaatacta tgttggccct
      781 cttcgacttt gttgctctgt cgttgaacgt tcaacggttg aacttgaact acgtatttgc
      841 gcgtaagagg acatgttgac tcacgggttg gcgaatagga agatacgagt cgatcgagct
      901 tagaggcaaa ccggatgatc tctcgcgagg aaaacaacct tttaactgaa accggtcccg
      961 gaactccttg tggagagctc atgcgccgct actggcagcc gatcgcttta tcagaggaat
     1021 taccgccggg aggcgcgccg ttgcgggtga atgttctggg cgaggagttg gtgctgtttc
     1081 gcgacgatca tgggcggccc ggtctactgg gtttgcattg ctcgcatcgt ggaaccgatc
     1141 tcagctatgg acggatcgaa aacggcgggc tcaggtgcat ctatcacggc tggctctatg
     1201 atgtttgcgg ccgcgtcatc gaacagcccg gcgagcccgg aggaggcgca aaccgggatc
     1261 agatccgtca tcgggcgcat ccctgcagag agaccggcgg tgtgatttta acctatatgg
     1321 ggccaggtga gccgtcgctg ctgcccgact atgaatttct ttcagtgccg gaagagcagc
     1381 gcacggttac aaaaattctg tacagctgca attatctgca gggtaatgaa ggcaacatcg
     1441 atccggtgca tttatctttc ctgcaccaat ttcttggcga tgccccagcg ccgctgcgac
     1501 gcgttgttcc gggcaccgaa acgacggata acaccttact ccgtaaagac atcgcgccga
     1561 caattgaagt cgagctcacc gatttcggtt tgagaatttg ttcactgcgg gaagccggac
     1621 aaaataaaac gtatttgcgt gttaccaact tcgtcatgcc caatctcgcc gcttttggcg
     1681 gttctacggt gggtgaaggg tactctgttc actggcacgt gcccatcgat gacaccagcc
     1741 attggaagta tgtcttcatg tttagccgcg aaaaggcgct gccgccggaa ctgatcaaca
     1801 agagccgtgt cgagctcggt gcggatttcc gcctgagccg cacgggcgcc aatcgctacc
     1861 agcaggaccg cgaatcaatg aagaccaaga cgtttaccgg cataggctat aactttcaag
     1921 cgcatgacgc atttgccacc gaaagtcagg gtgtgattca agaccgcacg gcggagcatc
     1981 tggtttcctc ggacaaagcg attgttgccg cgcgcaagct gttgctgaac gccatcaatg
     2041 acgttaagca gggccgggac ccgcagcacg tggtgcggga tccggcatca aaccgatttt
     2101 cgcacctgca agtattttcc gaggtaattg acgcctcggt agattggaag caacatacca
     2161 aggagcggat cgcgaccatc gagcgcatcc ggagtcgttg accgagcact gtaaaatgcg
     2221 gatgaatttt gtggaaaggt aacgttcaca tgacctggaa aaacatacta ggcttactgc
     2281 tttttctgtg cattctggcg ccggcggctg ccgcgcaaga gcgctacaat attgcgtatg
     2341 ccggcttcgc cggttttcag acgccggtct gggcgaccaa agatcttggt ttgctggcca
     2401 aatatggcat cgatggcgag gtcgttctgt tgccgggctc tacccgccag atccagggct
     2461 tggtcggcaa cagcgttgat ttcgctca
//
LOCUS       DASUTG010000077         3494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1049466, whole genome shotgun sequence.
ACCESSION   DASUTG010000077 DASUTG010000000
VERSION     DASUTG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049466"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1187)
                     /locus_tag="VF089_01675"
     CDS             complement(381..1187)
                     /locus_tag="VF089_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6172694.1"
                     /translation="MKKSFEIYLGGMLMALLLTCAMFAPELAPHNPNRQDLGNDLKVF
                     TPEHPLGTDKLGRDIMSRTIFGSRISLLVGVSTVGISISIGLFIGSLSGYFGGWLDQL
                     LMRAVDILLAFPGILLAIAFTALLGPGLDHVILALCLIGWTGYARLVRGEILSLRERE
                     YVQAALALGCSPARVITVHLLPNLLPLLLIQATFGMAAAITAEGSLSFLGLGVEPPTA
                     SWGSMLNEGRQFLLVAPHLTTYPGLAIMLTVLSLNLLGDALQNRLQSPTR"
     gene            complement(1171..2100)
                     /gene="nikB"
                     /locus_tag="VF089_01680"
     CDS             complement(1171..2100)
                     /gene="nikB"
                     /locus_tag="VF089_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HEX6172695.1"
                     /translation="MKRYFLNRLLLLVPTLFGALTLVFFLINLVPGDPVEVMLGETAS
                     SADKEELRRQLGLDQPVLTRYTKFLADLLRGDLGFSLYENAGVAKLIGARLPATLELT
                     TCAMIVAIIISVPLATLAALKPGTWIDRLALLFSLLGLSIPNFWLGPMLMILVSIQLG
                     WLPVSGRGGFSHVILPSLTLGMAMAAILTRILRTSLLRVVNLDFIQTARAKGLGEAVV
                     WLKHMLRNALIPVISIMGLQFGSLLAGSIITETIFSWPGIGRLTVQAIQTRDYPLVQG
                     CVLIVATGYLVVNFITDLAYSIVDPRISYEKKF"
     gene            complement(2097..>3494)
                     /locus_tag="VF089_01685"
     CDS             complement(2097..>3494)
                     /locus_tag="VF089_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172696.1"
                     /translation="ARISSLIYSSLLRLDNKSRLVGDLAEHWTIIDAQTYSFRIRKGV
                     TFHDGRPLTARDVKYTYDSIMDHRNRSPKRGPLRVLESVEQLGTQEIRFHLSEPYSPF
                     LEHCTMGIVPVGSGMPGENGSPHAPPGSGPFTLAGINLGEKIYLKANSSYWEGKPPLA
                     GLAFQIVPDAIVRVLEFKKGTIQLLQNDIEPDTLPWLEKNTDAIVETNPGTTFQYIGI
                     NLTHPILKHRDVRQALAHSIDRDAIIRHILKDQATAANGLLSPLNWAHDNSVPHWRFD
                     PAKAQRLLDQAGYPDPDGDGPRPRFKLSFKTTNIDLRRRIAETLKAQLSRVGVELEVR
                     AYEWGTFYGDVKKGNFHLYSLAWVGIADPDVYYNIFHSSSAPPNGDNRGGYNNPNVDH
                     LLEQARQESDPVRRKALYHHVQQIIHEDLPYIALWWVKNVVVRKPEIVGFTPYPDGDL
                     FSLRRTSLRQAPYLQ"
BASE COUNT          840 a          889 c          981 g          784 t
ORIGIN      
        1 gagcagcgtt aacccgttaa atttttcatc gagatactcg ttgatgatcg cggattcgta
       61 aatcactccg gcgccgtcga caagcaccgg aactttggca taaggattca gctcgagcaa
      121 atccttcggt ttgtttttca aattgacttg cacgagctca tacgaaatct tcttttcaga
      181 aaggacaatc cttgtcctgt gcgcataagg tcacgtatgg catgaataca gtttcacggg
      241 tgcctccttg ttgaggtcaa tatcaactgg gttcaaatgg ttcaagtcgc tcaatccgaa
      301 caaatctcga attgacgagc cgtagtgcag cattctttga aaagttctac ttcccaacca
      361 gtttcccctg tttgtccgac ttaccgtgtt ggcgattgca gccgattctg caaagcgtcg
      421 cccagcaggt tgaggcttaa taccgtaagc atgattgcca aacccgggta ggttgtcaga
      481 tgaggcgcca cgagtagaaa ttgcctacct tcgttcagca tcgaacccca tgaggcggtc
      541 ggcggctcaa cacctaaacc taaaaagctt agactccctt cggctgttat cgccgctgcc
      601 atgccgaatg tcgcttgtat gagaagcagc ggaaggaggt ttggcagcag atgaacggtg
      661 attacgcggg cggggctgca gcctaaggcg agtgctgctt ggacatactc ccgctctctt
      721 aagctcagga tttcaccgcg aacgagccga gcgtaaccgg tccagccgat taggcagagc
      781 gcaaggatga cgtgatcgag gccgggcccg agcagtgccg taaacgcgat ggcaaggaga
      841 ataccgggaa aggccagcaa aatgtccacg gcgcgcatga gcaactggtc gagccagccc
      901 ccgaaataac ctgagagcga gcctatgaag aggccgattg aaatcgatat tcctaccgta
      961 ctgacgccca ccagcaggga aatgcggctg ccgaatatcg ttcggctcat tatatctcgg
     1021 cccagtttgt ccgttccgag cggatgctcg ggggtaaaca ccttcaggtc atttcccaag
     1081 tcttgccgat tgggattgtg tggcgcaagc tccggcgcaa acatcgcgca cgtgagcagc
     1141 aacgccatta acataccgcc aaggtaaatc tcaaaacttt ttttcatagc tgatgcgagg
     1201 atcgacgatg ctgtaagcaa ggtccgtgat aaaattgaca acgagatagc cggtggcgac
     1261 aatcaacacg cagccttgta cgagcgggta atcgcgtgtt tgaatggctt gcacggtgag
     1321 ccgcccaatc ccgggccacg agaatatcgt ttcggtgata atcgacccgg cgagaagcga
     1381 gccaaactgc aaacccatga tggatatgac gggtatcaag gcgttgcgca gcatgtgttt
     1441 tagccaaact actgcctcgc caagtccctt ggcgcgcgcg gtttggatga aatccaagtt
     1501 gacgacgcgc agtaggctgg tgcgcaagat gcgcgtgaga atagccgcca tggccatccc
     1561 caatgtgagc gatggtaaaa tcacgtggga aaagccgcca cggcccgaga ccggtagcca
     1621 gccgagttga atcgaaacca ggatcatcag cattggaccc aaccagaagt ttggtattga
     1681 aagccccagg agcgagaaaa gcagcgccaa acggtcaatc caagtgccgg gcttgagagc
     1741 ggcgagtgtc gcaagcggaa ctgaaatgat gatggcgacg atcatggcgc acgttgtcag
     1801 ttcaagcgtg gctggcaacc gtgcgccaat gagtttggcg accccggcgt tctcgtaaag
     1861 agaaaatccc aagtcgccgc gcaatagatc ggcgagaaac ttggtgtagc gcgtcagtac
     1921 cggctgatcg agaccgagct ggcggcgtag ttcttctttg tcggcggagc tggcggtctc
     1981 ccccagcatt acttcgaccg gatctcccgg aacaagattg atcaggaaga aaaccaaggt
     2041 caacgcgcca aacagtgtcg gaacaagcaa cagcagtcga tttagaaagt atcgcttcac
     2101 tgaaggtatg gcgcctggcg gagtgatgtt cgtcttaatg aaaacagatc gccgtcgggg
     2161 tagggcgtaa agccgacaat ctcgggcttg cgcacgacga catttttcac ccaccacaga
     2221 gcaatatagg gtagatcttc atgaattatt tgctgcacat ggtggtacag cgcttttcgt
     2281 cggactggat cagattcttg ccgggcctgt tcgagcaagt gatcgacatt gggattgtta
     2341 taaccgccgc gattatcgcc gttgggaggt gcgctcgagg aatggaaaat attgtaataa
     2401 acgtccgggt cagctatgcc gacccatgcg agagagtaaa gatggaagtt accttttttg
     2461 acatcgccgt aaaacgttcc ccactcataa gcgcgcacct caagctcgac gccgacgcgg
     2521 cttaattgcg ctttcaacgt ctcggcaatt cttctgcgca aatcgatgtt cgtggtcttg
     2581 aaagatagct tgaagcgcgg ccgcggcccg tctccatcgg gatccggata ccccgcctga
     2641 tccaaaagcc gctgggcttt ggcaggatca aaacgccaat gcggaaccga attgtcgtgc
     2701 gcccagttga gcggggaaag cagcccattg gccgctgtgg cttgatcttt gagaatgtgc
     2761 cggataatgg cgtcgcgatc gatggagtgg gccaaggctt gcctgacgtc tcggtgtttc
     2821 agtattgggt gcgtgaggtt tatgccgatg tactgaaacg tggttcccgg gttggtctcg
     2881 acgattgcat cggtattctt ttccagccat ggaagggtgt ccggctcgat atcgttctgc
     2941 aatagctgga tggttccttt tttgaactct aaaactcgaa caatggcatc cggcacaatt
     3001 tgaaaggcca gtcccgccaa cggaggttta ccctcccagt aagatgagtt tgctttgaga
     3061 tatattttct caccgagatt gatccccgcc agagtaaatg ggccggaacc gggtggtgca
     3121 tgggggctac cattctcccc tggcatcccg ctgccaaccg ggactattcc catggtgcaa
     3181 tgctcaagaa aaggtgagta cggctcggat aaatggaagc ggatttcttg agttcctagc
     3241 tgctccacgg attctagaac ccgcagcggc ccgcgctttg gcgagcgatt gcgatgatcc
     3301 atgatcgagt cataggtgta cttgacgtcc cgggctgtta agggtctgcc gtcgtggaag
     3361 gtaacgcctt tacgaattct gaagctgtag gtttgtgcgt cgattatcgt ccagtgttcg
     3421 gcaaggtctc ccactaagcg cgatttgtta tccaagcgaa gaagcgaact atagattagc
     3481 gagctgatac gcgc
//
LOCUS       DASUTG010000078         6225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1791126, whole genome shotgun sequence.
ACCESSION   DASUTG010000078 DASUTG010000000
VERSION     DASUTG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791126"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..735)
                     /locus_tag="VF089_01690"
     CDS             complement(205..735)
                     /locus_tag="VF089_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005974207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172697.1"
                     /translation="MAKKVKSQPDWLKSDGLEELYSISGCISEYFCDYIPHWKHNGFW
                     LFDSPAEITAVAAAESLNMSEQRIFYYELYENQFDEQRKIWQPFQADKSFKTEVSAPK
                     SKKLEGFDVVTFSVQTSPECSPLSCNRLAESIPVNAHCLLPSLEEAIRLVESGAFDNS
                     EPGPFRIFAVYSCDAA"
     gene            complement(1222..1437)
                     /locus_tag="VF089_01695"
     CDS             complement(1222..1437)
                     /locus_tag="VF089_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6172698.1"
                     /translation="MLLRKRGEGKIINIGSIAGEMGIRHVAPYAASNRGVLGVTSTLA
                     VKWACEGICVNCLAPEYIETDLTSDVR"
     gene            complement(1579..2400)
                     /locus_tag="VF089_01700"
     CDS             complement(1579..2400)
                     /locus_tag="VF089_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX6172699.1"
                     /translation="MPIITIYQGASGEGQELAESLAEALGYRCVGRDEVLVEASRRYR
                     IPEAKLNAVVEKGPHWWERLLQDLRPYRTALQASLCELAHDGKVVYHGHLGHELLSGI
                     GHVLKVLLTAPIESRVGQVRVRQSLTEAAARHYVEEVDKARSRRLMAMFGADWRDPNR
                     YDLVLNMGKMSREGAKRLIIEAAKLEEFQPTPASNQAFNDLALGSRVLATLFASHDVR
                     GSALEVRAEGGHIHVKGMVDYGSEDQVLRLIRNVPGVTKVTSDLYSPPPEGYLGP"
     gene            complement(2581..2901)
                     /locus_tag="VF089_01705"
     CDS             complement(2581..2901)
                     /locus_tag="VF089_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6172700.1"
                     /translation="MSKFLMQVSFTREGTKGLTEEGGTKRRQKVEQFFGSVGGKLEAL
                     YFAFGETDVFAIVDFPDNVSAAAISLAANSTGAVHAKATVLITPEEMDQAAEKSEGLR
                     PPVS"
     gene            complement(3378..4490)
                     /locus_tag="VF089_01710"
     CDS             complement(3378..4490)
                     /locus_tag="VF089_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX6172701.1"
                     /translation="MFICLSPGGQSITQGETPKQKLLVGTVDGIFAFHKRNGSWEGQG
                     TMVPGKHFSSLIFEPVTRMLFGGTYSGEIYASVDHGQTWERRDNGIDDKEIYSLATQV
                     VDGKPRLYAGTQPAHLFYSDDLGKSWTELPGLRQVPGVEKWTFPGPPHLAHAKSITFH
                     PKDPNIIHVAIEVGGFLKSTDGGKTWSTVDNINPDAHRVLIPTNDPSKLYGTAPTTNC
                     GPETAAGFCVSEDGGESWKSMTPRDFRIGYVDPFFVHPSDPNLLFVAGAKTGPGTWRK
                     LHTADARIARSRDGGKTWDILTGGLPEHIRANIEGMAMDVWNGGYALFAGTTDGDIFY
                     SDDEGEHWETIVSGIGPVSKSHHHINLSLDEQAAHH"
     gene            complement(4974..5525)
                     /locus_tag="VF089_01715"
     CDS             complement(4974..5525)
                     /locus_tag="VF089_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX6172702.1"
                     /translation="MLQPLCPNCSRDYTKRVSRIGFKERLLSIIYVYPFRCQLCSYRF
                     RFMQWGVRYKRVEEDRREYNRMPASFPVSFACDGIEGTGHVVDISLNGCSLQADTVIT
                     EGSIVRMALQMSEELAPISIEAALVRDINRNRIGIEFLRFQYHDRERLQIFIRRLLNG
                     IPEPSPRPSLLINALKRTRHIGA"
     gene            complement(5727..>6225)
                     /locus_tag="VF089_01720"
     CDS             complement(5727..>6225)
                     /locus_tag="VF089_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172703.1"
                     /translation="MVMGYKGGAEQDLAFERGEVVCRSLSIPTFFAREPFITWRKKNV
                     VRILMQTGRKRDSRMPDVPTIYELMEEYKTPPQTRSLVTAILASGDLGRPFIAPPNLP
                     ADRLKILRDGFQKTMADPAFLADVKTRRLAADPDFGEQLETIAKEAVNQPREVVERMK
                     KLLSD"
BASE COUNT         1437 a         1749 c         1620 g         1419 t
ORIGIN      
        1 gcttctgctt gctctgccag gtcaaggatc tgacgaaagc tcgggatctc ttgtgtcatg
       61 acgatttccc gtgttgggag taggaatcca aaagccgtca tggttttgtc tccttagagg
      121 tataggcggg tggtaacatt cgtgatgacg ggcaccatac ggagcgcaga gcggcgaacg
      181 gacggtgcca ctgtccaacg cttgttaggc tgcatcgcaa gagtaaaccg caaagatgcg
      241 gaacggacca ggctcgctat tatcgaacgc gccactctca accagtcgaa tagcttcctc
      301 aagagaaggt agcaagcagt gcgcatttac cggaattgac tctgccaacc tgttgcacga
      361 tagcggtgaa cattcagggc tggtttgtac gctaaacgtc actacatcaa agccttctag
      421 ttttttcgat tttggtgcgc ttacttcggt tttgaatgac ttatcagcct ggaagggctg
      481 ccaaatcttt cgctgctcat cgaactggtt ctcgtataac tcgtagtaga agattctttg
      541 ctccgacatg ttcaaggatt cggccgcggc caccgccgtg atttccgccg ggctgtcgaa
      601 caaccagaag ccgttgtgct tccaatgcgg gatgtaatcg caaaagtatt cggagatgca
      661 gccgctaatg gagtagagtt cctcaaggcc atccgacttc aaccagtcag gttggctttt
      721 aacctttttt gccatgtagc ccgctgggac catgcagttt aatcatcaag tatactgccg
      781 actattccgg caggttatac gggaacccgc aaacaattaa gagcaaactc agctgcaaca
      841 tcggttaaaa aaccgctcct tgtcaatcgc tttggacaaa cgccgctcta tggcactgtg
      901 tccagattca gtgagcgact tatccagaca aaccaatccc agacgctcta gaactgaact
      961 gaacggccgt tcaaaataat ctcgtcgccg ctcgaggctc gttcacgatt ctaagttgca
     1021 gccgttgcct ctgttcattc ccgttcgaag ctgttcaacg aggtcacgcg atcagaaagc
     1081 cgcagtctac gactatagtt tgcccggtga ggtaatcact agcaggcgct gcaagaaaaa
     1141 cagcagcacc gataaggtcg gccggagaaa ccacgccggc ccatcgaaac ccgattctga
     1201 aacgcggtcg tgaaggcggg atcatcgaac atcgctcgtc agatcagttt cgatgtattc
     1261 aggggcgaga cagttaacgc agatgccttc gcacgcccac ttgacggcca aagtgctcgt
     1321 cactccgagc acgcccctgt tgctcgccgc atagggagca acgtggcgaa tgcccatctc
     1381 gccggcgata ctgccgatgt tgatgatctt gccttcacct cgctttctca ggagcaaagc
     1441 ggcgagtctc gatccaatat cttccgacgg cccgggacga gaagaacaga accgcaacca
     1501 tgggcgtagc agtttgtcgt gtgggaaaat agacaaacgt gatgggtctc tgcagcagtc
     1561 aatttggatc ctcgtcactc aaggccccaa atacccttcc ggcggagggg aataaaggtc
     1621 ggacgtcacc ttggtcacac ccggcacatt tctaattagc cttaggactt ggtcttccga
     1681 cccatagtcg accatgccct tgacgtgaat gtggccacct tcagcgcgaa cttcgagtgc
     1741 cgatcctcgt acatcgtgag aggcaaacaa tgtggccagc acccgggaac cgagagcaag
     1801 atcgttgaac gcctgattgg atgcgggagt gggctggaac tcttcgagct tcgccgcttc
     1861 gataatcaaa cgcttcgccc cctcacggct catcttgccc atattaagaa ccagatcgta
     1921 acgattagga tcgcgccaat ccgcgccgaa catcgccatc agccggcgac tgcgcgcctt
     1981 atcgacctcc tcgacataat gacgcgccgc tgcttccgtg agactttgcc gcacccggac
     2041 ttgtccgacg cgagattcga tgggcgcggt caaaagtact ttcagaacat ggccgatacc
     2101 tgacaaaagc tcatgaccca agtggccgtg gtaaacaacc tttccgtcgt gagccagttc
     2161 gcatagcgaa gcctgaagcg cagtccgata gggccgcaga tcctgcagca gacgttccca
     2221 ccagtgcggc cctttctcga ccaccgcatt gagtttcgct tcaggtatac gatagcgccg
     2281 gctcgcctcg acgagcacct cgtcgcgccc gacacaacga tagccgagag cctcggccaa
     2341 actttcggcc agttcttgac cttcgccgga agcgccttga tagattgtaa tgatcggcat
     2401 agaagcaccc tcctcgaagg tttatacggt acccaactcc cagcaaaaat ttccaaaaag
     2461 caacgccgaa ctctacacgg actttacaat gttccggcaa ctccagcgcg aatgcgtgaa
     2521 tcgctttcga tgccgtacag gtgtgcttct cacattttct cttgccttgt cataatcgct
     2581 ctacgagaca ggcgggcgaa ggccttcgct cttctctgcc gcctgatcca tctcttcggg
     2641 cgtgatgagt accgtcgctt tcgcatgaac ggctcccgta gaatttgcag ccagagaaat
     2701 tgccgcggcg ctaacgttgt ccgggaaatc gacgatagca aaaacatctg tctcgccaaa
     2761 tgcgaaatat aacgcttcca atttgccacc aacgctacca aagaattgct cgactttttg
     2821 gcggcgcttg gtaccgcctt cttcggtaag tcctttggtt ccttctctgg taaatgatac
     2881 ctgcatgaga aacttagaca taaaacctcc ttgtcatgga acattattat cctgctcctc
     2941 ccgtccgtcg ggaaggttcg cgtttcttca gcggatgtcg tcgattactg tcactcgagt
     3001 gggtcgtggc accaactttc ctaaaaaata accaataaaa aggccagcac accctggcgc
     3061 taatagcacc cgagcggctg gccctatact cctttactca tttcccctcc gaggaacggt
     3121 atccgttcgc tcgccggcct cagatcaagc cttatcgatg atggggaagc ttttacgatc
     3181 gggaaactat cacccgcgga aagtctgtca agcactttct cgccgtatag caagtctcaa
     3241 acgcaagttc gtgccgtagc ttatgtaccg cacggccgtt cgccccgtgc aactgcatcc
     3301 aaaccagagt taaggagaag aagatgggcg ggagcagcat gccgctcccg ccgaatgcaa
     3361 gacgctttat ttaactacta atgatgtgcg gcttgttcgt ccaaagacag attgatgtga
     3421 tggtgcgatt tcgaaaccgg gccgatgccg ctaacgattg tctcccaatg ctcgccttcg
     3481 tcgtcgctgt aaaaaatatc gccatcggtt gtgccggcga ataaagcata gccgccgttc
     3541 caaacgtcca tggccatccc ttcaatgttg gcgcgaatgt gttcgggcaa gccgccggtg
     3601 agaatatccc aagtcttgcc gccgtcccgg ctgcgcgcga tgcgcgcgtc ggcggtgtgc
     3661 agtttgcgcc aggtgcctgg gccggtcttg gcgccggcaa cgaacaaaag attgggatcg
     3721 gagggatgaa cgaaaaatgg atcgacgtag ccgatgcgga aatctctcgg cgtcatgctt
     3781 ttccagctct cgccgccatc ttcgctgacg caaaaccccg cggccgtttc agggccgcaa
     3841 ttggtcgttg gcgcagtgcc gtagagcttg ctcggatcat tggtcggaat cagcacccga
     3901 tgcgcgtcgg ggttgatgtt gtcgaccgtg ctccaggttt tgccgccgtc cgtgctcttg
     3961 agaaatccgc ccacttcgat ggccacgtgg atgatgttgg gatccttggg atgaaaggtg
     4021 atgctcttgg catgcgccag gtgcggtggg ccggggaaag tccacttctc gacaccaggc
     4081 acttggcgaa gccccggcag ctccgtccag cttttgccga gatcgtcgct ataaaacaga
     4141 tgcgccggct gggtacctgc gtatagccgc ggcttgccgt caaccacttg ggtcgcaaga
     4201 gagtaaatct ccttatcgtc tattccgttg tcacggcgct cccaggtctg gccatgatcc
     4261 acgctggcgt aaatctcacc gctataagta ccgccgaaca gcattcgggt tactggctca
     4321 aagataagcg agctgaaatg ctttcccgga accatggtgc cctgaccttc ccatgagccg
     4381 ttgcgcttgt gaaaagcgaa aatgccatcc accgtaccga cgagtaattt ctgctttggc
     4441 gtttcgcctt gagtgatcga ctgccctccg ggtgatagac agataaacat agcgtggcct
     4501 cctttagagt tggaagagta ggttgacttg tccgacttga agccactcta ctgaaccgcg
     4561 cgcggcttcg tcaagccaga gaatcagcca gtccaactat gttttccttg tggcgccgag
     4621 ataaagccag tggtggtttc ctcgcgctac gctgtgaatg gactatccat taaagatgtc
     4681 gccaagactt tcataaagcg ctggtgaccg ccggcgcgga accatcaagc ggcaaagtcg
     4741 cggcgcgtgc ttacccacat gttgtgcctg ggctaatcca tcaaaatcga ggcgaccggc
     4801 tcggcttcga agatccgact ctgaagctca ccaatatagc tttgaagagc ggttggaaat
     4861 tgacttggtg atggcaactt ctttttctct gattgccatc cagaaaatcg ctccgggcct
     4921 agacacgaaa cggagcggcg atcgtttgat tagaggaacg gaagaacaat cgatcacgca
     4981 ccgatgtggc gggtgcgttt cagagcattt attagaagtg acggcctcgg agaaggttca
     5041 ggaattccgt tgagcaaacg acgaataaaa atctgcagac gttcacggtc atgatactga
     5101 aaacgtaaga actcgatgcc gatacggttt ctgttaatgt cgcgcaccag tgccgcctcg
     5161 atactgattg gcgctagctc ttcggacatt tgcaaagcca tccgcacgat gctgccttcg
     5221 gtgatcacgg tatcagcctg gagcgagcaa ccgtttagag aaatgtctac cacgtgccct
     5281 gtcccctcta tgccatcgca ggcaaacgaa accggaaaac tcgccggcat ccggttatac
     5341 tcgcgccgat cttcctcaac tcgcttgtaa cgcacacccc actgcatgaa gcgaaatcgg
     5401 taactgcaca gctggcacct gaaagggtag acataaatga tgctcagcag gcgttctttg
     5461 aacccgatcc gcgaaacccg tttggtgtaa tcgcgcgaac aattcggaca tagcggttga
     5521 agcattgact cttttcccgt ccttaacctt gccagcggta gtcttcacaa gggtaaggac
     5581 ttgccaatgc aagtcatgtt ccgccagata caacgggagc cgaccgcgga tgatcagcca
     5641 gggtttagct gattgtgtgg aaatcgccaa aagaacggag gagtctatcg tgaaggcgcc
     5701 ccggaggagc gccagcgcgg gcctgctcaa tcgcttagta gcttcttcat ccgctcgaca
     5761 acttcacgag gttgattgac cgcttctttg gcgatggtct cgagctgttc gccaaaatcc
     5821 ggatcagctg ccaatcttcg ggttttcaca tcagccaaga aagcgggatc ggccatggtc
     5881 ttctggaaac cgtcacggag aatcttcaat cgatcggccg gcaggttcgg cggcgcaata
     5941 aacggtcgtc ccagatctcc ggatgccaag atcgctgtca ccagagatcg ggtttgcggc
     6001 ggtgttttgt actcttccat cagttcatag atagtcggca cgtcgggcat gcgcgaatcg
     6061 cgtttgcgac cggtttgcat cagtattcga accacattct tcttgcgcca ggtgataaat
     6121 ggttcacggg caaaaaaggt tggaatgctc agcgaacggc aaacgacctc gccccgctcg
     6181 aacgccaagt cctgctccgc gccgccttta tagcccatta ccata
//
LOCUS       DASUTG010000079         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1049655, whole genome shotgun sequence.
ACCESSION   DASUTG010000079 DASUTG010000000
VERSION     DASUTG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049655"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF089_01725"
     CDS             complement(<1..388)
                     /locus_tag="VF089_01725"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX6172704.1"
                     /translation="MEFLQCLRLGKQITGHCSQQHHRRQDRHWAKDESHRSTLLSALL
                     SHFLSDSTVKSRTLNFSPDAAADRPLRTISKVGGVEEDSVFYYRIHSPVLLVEFDHQA
                     GQAFPQNKEHYKEHIHVIVRTPNGNDY"
     gene            284..1288
                     /locus_tag="VF089_01730"
     CDS             284..1288
                     /locus_tag="VF089_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172705.1"
                     /translation="MRFVFRPMTILTAVVLLGTVPSDLFSQTKALKELNISYPLGGSS
                     SFFWVAYRSGSFERQGLRLKPIFIPGGVTALQSLLAKEVSIQLTAGPAAIRAWARGAR
                     EVTFIGAVGNRLDYIIVAHPSVRNVNDLKGKRIGVSQLGASPDFIARMGLRRLGLNPE
                     KDVTIVPIGSPGERWSALTAGHVQASLFQAPFTLRARKAGYVSLLDFATQDFEYILSG
                     VLTTRSFINSDRDTVMNFMRGLADGMDFYRDERNREKTMSYLREYYRSNANEELEETI
                     NMYSRVTPGLPAVTAKSIENVIVSDGDLSGMGLKAADMLDLSFLQKLAEERKAKGKES
                     "
     gene            complement(1354..2304)
                     /locus_tag="VF089_01735"
     CDS             complement(1354..2304)
                     /locus_tag="VF089_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX6172706.1"
                     /translation="MGYLVCALAGCSATSWKSPLGQNHPLTGRIWDVSFAQFIDRETL
                     VRRLARAHFVLLGERHDNPDHHMLQAEVLRSLIAVGRRPAVGFEMLGLDDANVIAAHL
                     AAAPNDAAGLGRAVNWNQRGWPDWAMYQPIAEAALQAKLRIVATNLPLATARRMTRDG
                     LAALEPPVVRELDLDRQLPEPVFARMAAEIRDAHCGYASETTVKAMVHVQRARDAQMA
                     QSLIAAGDPGGAILVAGAGHVRADYGIPVYIRAKAPDKRVISIAFVEVDKQKTNPQSY
                     ALPYPDGRLPFDYVWFTPRVDDEDPCEKFKSQFDRMKKAQ"
     gene            complement(2474..3658)
                     /locus_tag="VF089_01740"
     CDS             complement(2474..3658)
                     /locus_tag="VF089_01740"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX6172707.1"
                     /translation="MSVSEAKPTNQPAAIAVARRNGRPLRIGGGPPNYADNAAKALSE
                     NWKGVTTDGSPLQELFPIQQTGVSTQPIREAAEAFLASLDAEQRRHTLFPIDAPEWRL
                     WSNIHRTIMRHGMPLFAMTDSQLDHALALLRESLSAQGFQTARDIMRLNETVMEMTGK
                     LDEFGEDLYWLSIMGTPSEKEPWGWQIDGHHLNVNFFVLGDQVTMTPAFLGSEPVHAL
                     SGKYAGTRVFQAEEANGLALMRSLTGDQRGKAVIGVSLPKDLFTTAFRDNLELRYQGI
                     RYDQLNGAQHDLLVQLVEIHIGKLRPGHSAIKLAETHSHFKDTYFAWIGGVEEDSVFY
                     YRIHSPVLLVEFDHQAGQAFPQYKEHYKDHIHVIVRTPNGNDYGRDLLRQHYQRSGHH
                     RV"
BASE COUNT          792 a         1075 c         1105 g          856 t
ORIGIN      
        1 cgtaatcgtt gccgttgggc gtccgcacga tgacgtgaat atgttctttg taatgctctt
       61 tgttttgggg aaaggcctgg ccggcttgat gatcgaactc gaccaacaac accgggctat
      121 ggatgcggta atagaaaacg ctgtcttctt ccacgccgcc gaccttgctt atggttcgaa
      181 gcggtctgtc ggccgccgcg tcaggcgaaa aatttaatgt cctggacttt actgtgctgt
      241 ctgagagaaa gtgcgaaagt aaagccgaga ggagggtaga tcgatgagat tcgtctttcg
      301 cccaatgacg atcctgacgg cggtggtgct gttgggaaca gtgcccagtg atctgttttc
      361 ccagacgaag gcattgaagg aactcaatat ctcgtaccct ctcggcggtt cgagcagttt
      421 tttttgggtt gcctatcgtt caggttcctt cgaaagacag ggtctgagac ttaaaccgat
      481 cttcatcccc ggaggtgtca ccgcgctcca gtctctgctg gcgaaggaag tgtcgattca
      541 gttgaccgcc ggtccggccg ccattcgcgc ctgggctaga ggcgccagag aggtcacctt
      601 tattggagca gtcggtaacc ggctcgatta cattatcgtt gcccatccct cggtgcgtaa
      661 cgtcaatgat ttgaaaggta agcgaatcgg cgtgagtcaa ctcggtgcca gccccgattt
      721 catcgcccgg atgggactgc ggcgcttggg gctcaatccg gaaaaggacg ttaccatcgt
      781 gccgattggc tcgcccggag aacggtggtc ggcactgaca gccggtcatg tccaggcctc
      841 gctcttccag gcgccgttca cgctgcgggc gcgcaaagcc ggctatgtgt ccctgctcga
      901 ttttgccacg caagattttg agtacatcct ctccggcgtt ctgaccacgc gctccttcat
      961 taactcggac agagacacgg taatgaattt catgcgtggg ttggcggacg ggatggactt
     1021 ttatcgcgac gagagaaatc gcgagaagac aatgagctat cttcgtgaat actatcgatc
     1081 caatgccaac gaagagctgg aagagacgat caacatgtac agccgggtca ctcccgggct
     1141 tccggcggtg acagcgaagt ccatcgaaaa cgtcatcgtc agcgacggcg atctttccgg
     1201 catgggcctc aaagcggccg acatgctcga tctgtcattc ttgcagaagc ttgcggaaga
     1261 aagaaaagcc aaaggcaaag agtcctaaag tagccaggcc gctgaagccg tccattcgag
     1321 aagagacatc ggtggccttg acccgtgagc cgctcactgg gctttcttca ttcggtcaaa
     1381 ctgggacttg aatttttcac aggggtcttc gtcgtcgacc cgtggcgtga accaaacgta
     1441 atcaaaaggc aatcggccat cggggtaagg cagcgcataa ctctgcgggt tggtcttctg
     1501 tttatcgact tcgacgaaag ctatgctgat cacccgcttg tccggcgcct ttgctctgat
     1561 gtaaacgggt atgccgtaat cggcgcggac atgacccgct ccggctacca ggatcgcacc
     1621 gccgggatct cccgctgcaa tcaagctctg cgccatctga gcgtctctgg cgcgctgtac
     1681 atgcaccatc gccttcacgg ttgtctcgga cgcatagccg caatgagcgt cgcgtatttc
     1741 agccgccatc ctcgcgaaca caggttcggg cagctgccgg tcgagatcaa gctcgcgcac
     1801 cactggcggc tcgagggcgg ccaagccgtc cctggtcatc cttcgcgcag tcgcaagcgg
     1861 tagattggtc gcaacgattc gtagctttgc ttgcagcgcg gcctcagcga tgggttgata
     1921 catcgcccaa tccggccatc cacgttggtt ccaattgact gccctgccaa gcccggcagc
     1981 atcattaggc gcggcggcga gatgtgcggc gataacgttg gcgtcgtcga gaccgagcat
     2041 ttcaaaaccc actgcgggac gtcgtccaac ggcaatcaaa ctccgaagca cttcggcctg
     2101 cagcatgtga tgatccgggt tgtcatggcg ctcgccgaga aggacgaagt gagcgcgagc
     2161 caaacgtctg accagagtct cgcgatcgat gaattgcgcg aaggagacgt cccaaatacg
     2221 tccggtgaga ggatgatttt gacctaaggg tgatttccac gatgtggccg agcaccctgc
     2281 cagcgcacac actaggtagc ccacgatcag tgcaagtaga ttcgtcgcgt gatgtcttac
     2341 tccgaaaccg cgtcggcgac atccctcgtg catctgcaag tgaaaagtcc gatccttccg
     2401 aaataaccgt gccgtataaa tctcagcaca aaaacatgcg ccggttaacc ttgctcgctt
     2461 ccgaatccca atttcaaact cgatgatgcc ccgaccgctg gtaatgctgg cgcagcagat
     2521 ctctgccgta gtcgttgccg ttgggcgtgc gcacgatgac gtgaatatga tctttgtaat
     2581 gctctttgta ttggggaaag gcctggccgg cttgatgatc gaactcgacc aacaacaccg
     2641 ggctatggat gcggtaatag aaaacgctgt cttcttccac gccgccgatc caggcaaaat
     2701 aagtgtcttt gaagtgcgaa tgggtttcgg ccaatttgat cgccgaatga ccgggccgca
     2761 gtttgccgat gtgaatctcg accagctgca ccagcaaatc atgctgcgcg ccgtttaact
     2821 ggtcataacg gatgccttga tacctgagtt ccaggttatc tcggaaagcc gtggtgaata
     2881 ggtccttggg caggctcacg ccgatcaccg ctttgccgcg ctggtcgccg gtcaggctgc
     2941 gcatgagcgc gaggccattc gcttcttcgg cctgaaagac ccgtgtgccg gcgtatttgc
     3001 cgctcaaggc atgcacgggc tccgagccca gaaatgccgg cgtcatggtc acctgatcgc
     3061 cgagcacgaa aaaattgacg ttcaaatgat gaccgtcgat ctgccagccc cagggctctt
     3121 tttcagacgg tgtgcccatg atgctgagcc aatacagatc ctcgccgaac tcatcgagct
     3181 tgccagtcat ttccatgacg gtctcattaa gccgcatgat gtcccgggcg gtttgaaatc
     3241 cttgggcgct gaggctctct cggagcagag ccaacgcatg atcaagctgg ctgtcggtca
     3301 tggcaaacaa cggcataccg tgacgcatga tcgtgcggtg gatgttgctc cacaagcgcc
     3361 attcgggcgc atcgatgggg aagagcgtgt ggcggcgctg ctcggcgtcg agcgaagcga
     3421 gaaacgcttc agcggcttcg cggatcggct gcgtcgaaac tccggtctgc tgaatcggaa
     3481 acagttcctg aagcggcgag ccgtcggtgg taacgccttt ccagttttcg ctgagcgcct
     3541 tggcggcgtt gtcggcatag ttcggcgggc cgccgccgat gcgcaatggc cggccattgc
     3601 gccgcgccac ggcgatggcc gcaggttgat tggtcggttt ggcttcgcta acgctcatca
     3661 cggttctcct tatctgatga cagtaaaatt acgcgctccc ctttacccat gtcaaatcgc
     3721 gccggtggat cttcgcattg actggtttcg gctcaggtgt tacgtaagct tgactgcggg
     3781 gccacgcggt ccaacggctg ttcagtctcg ccgattttcg gctatgcg
//
LOCUS       DASUTG010000080         3271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1791223, whole genome shotgun sequence.
ACCESSION   DASUTG010000080 DASUTG010000000
VERSION     DASUTG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791223"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..787)
                     /locus_tag="VF089_01745"
     CDS             complement(263..787)
                     /locus_tag="VF089_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172708.1"
                     /translation="MKDLSPKQSISLIVAFFAGFFTLFLYQRWNPTIAPVDESTTLKA
                     VRLQQPPALPSFAVTFAHLPASEQAEPPVLQAVDTEKIRALAGKPARIRGRIFRVGHS
                     AKSNTYFLNFGPSRSALTAVIFASAVDLFEQGKLPPKGFDGREVEVHGEVKDHPQYGL
                     EIVLNNTSQIMILK"
     gene            complement(784..1320)
                     /locus_tag="VF089_01750"
     CDS             complement(784..1320)
                     /locus_tag="VF089_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6172709.1"
                     /translation="MKPSRFLVAGAILFGFVASASADMAVQACFSPEGKCSTHIVREI
                     ENARRELLIAVYAFTSDDLAGAVVQARKRGLMVQVIIDREFDSRNDKSKGKFLEVQRI
                     PVRRLSGSKPPHSAREVGLMHQKFAIIDRRIVFTGSYNWTQAADSFNDENLLVFRDAG
                     PLAEEYRKIFSRLWERRS"
     gene            complement(1317..2684)
                     /gene="holA"
                     /locus_tag="VF089_01755"
     CDS             complement(1317..2684)
                     /gene="holA"
                     /locus_tag="VF089_01755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX6172710.1"
                     /translation="MKRNFDSFLTDVGKGDGPPFVLLFGDDLQVQSGCKLLIDVVVPA
                     DQREFNLERFDGRSAAWDQIEASLLTPPFLPGKKLVWVESAPYFLSRENKTELGVKIM
                     HLWRDDKRDDAGKLLLSLLAIEGWSQQQWEQLEVSGGAAVFELLDAQDHDDREAVAAL
                     VAYCKSRGIELRGGRESEENAIRKLLTGGLPSWDFLLLTAAQVDRRTRLYKQFEENGA
                     VFFLGLERDRYGKITEDKLREFIDRQLSLFNKRVEPKARELILLRAGDDLRALQQELN
                     KLALYVGEHAVVRADDVETIFADQIEGWIFDLTRFIAERNATAALCQLARLIAQGDHP
                     LKLLSTIATEVRKLLSARQILENDLRGRWKRGMTYKQFEHAILDQRTTLLTRNPYADY
                     LCFQRAESFSLSELLSYMSGIHDADLRLKSSGTNPKLVMERLILQMCLGGRAVPKYAG
                     RTAAI"
BASE COUNT          777 a          876 c          792 g          826 t
ORIGIN      
        1 aatccatacg tgaatagtaa tcctgcgata gtcagatgta agttaagtct tttcagcttt
       61 ttggcctctc ttccggggct tcttcactga gtgcgctggt cgctcgaagc atgaaaatag
      121 agccacttta taccatgctt agcgatgtta cggtagcttt ttgtctccca agggcatttc
      181 caattttgcg gggttacatt ccttcggggc gagtatagtt gaaggcccgt ctgctagcgt
      241 atcgactgcg gctcagccga aattatttta aaatcatgat ttgtgacgtg ttattcaaaa
      301 ctatttccaa cccatattgc ggatgatcct ttacctcacc atgcacttcc acctccctgc
      361 catcaaaccc cttcggcggc agcttacctt gctcgaaaag atccacggcc gacgcaaaga
      421 tgactgcggt cagtgcggac cgcgaaggtc cgaaattgag aaaataagta ttgcttttgg
      481 ccgaatggcc aacgcgaaaa attcggccgc gaatccgcgc aggcttaccg gccaaagcac
      541 ggatcttttc agtgtcgacc gcttgcaaaa caggcggctc ggcttgttca ctggccggca
      601 aatgcgcgaa tgtgacggcg aagcttggaa gagctggtgg ctgttgaaga cgcaccgcct
      661 tcaacgtcgt ggactcgtct actggcgcga tcgtgggatt ccagcgttga taaagaaaaa
      721 gggtgaagaa gccggcgaaa aacgccacta taagcgagat cgattgtttc ggcgagaggt
      781 ctttcaagac cttctctccc acaaacggct aaaaattttt cggtattcct ctgctagtgg
      841 cccagcatca cgaaaaacta aaagattctc atcattgaag ctgtcggcgg cttgggtcca
      901 attataagac cccgtgaaaa caattcggcg gtcgatgatg gcgaatttct ggtgcatgag
      961 cccaacctcc cttgccgaat ggggcggctt gctaccggag agacgtcgta ctggaattct
     1021 ctggacctcc aaaaatttgc ctttcgactt atcattgcgc gagtcgaact ctcgatcgat
     1081 gatgacctgg accatgaggc cccgcttcct ggcttgtacc accgcgccag ctagatcgtc
     1141 gcttgtgaac gcgtagaccg caatcaggag ttctcttcta gcattctcga tttctcgtac
     1201 gatatgggta gagcacttgc cttcgggact aaagcaagct tgaaccgcca tatcggcgct
     1261 agcggaggca acaaagccga ataaaattgc acctgcaact aagaaccgtg atggcttcat
     1321 atggccgccg tccggcctgc gtattttggc acggcccgcc ctcctaagca catctgcaaa
     1381 atcagccgtt ccatgactag tttcgggttg gtcccgctcg acttcaagcg cagatccgca
     1441 tcgtgaatac cgctcatgta tgaaagcaac tctgagagcg aaaagctttc agctctttga
     1501 aagcacagat aatctgcgta cgggttgcgc gtcagtaatg tcgtcctttg gtcgaggatc
     1561 gcgtgctcaa attgcttata ggtcataccg cgcttccacc gaccgcgcaa atcattttcg
     1621 agaatttgtc gggccgataa aagcttccgc acttcagtcg cgatggtgct cagtaatttc
     1681 agtgggtgat cgccttgcgc gatcagacga gctagttggc agagagccgc tgtggcattg
     1741 cgttcggcaa taaatcgggt gagatcaaat atccaacctt cgatttggtc cgcgaagatc
     1801 gtctcgacat cgtcggcacg gacaaccgcg tgttccccga catagagcgc caacttgttc
     1861 aactcttgtt gaagcgcgcg tagatcatcc cctgcccgga gcaaaatcaa ttcgcgagct
     1921 tttggttcga cccgcttgtt aaataaactc aactggcggt caataaactc ccgcagcttg
     1981 tcttcggtga tcttgccgta gcgatcacgc tccagaccaa gaaaaaatac tgcgccgttt
     2041 tcctcgaact gcttataaag ccgggttctc cggtccactt gggctgcagt gaggagcaga
     2101 aaatcccatg acggcaaacc tccggtcaaa agttttcgaa ttgcgttttc ttcggattca
     2161 cggcctcctc taagctcgat tcctctgctt ttacaatatg ccaccagcgc ggcgactgct
     2221 tcccggtcgt catgatcttg cgcgtctaac agctcaaata ctgctgcacc cccggacact
     2281 tcaagctgtt cccattgttg ttggctccag ccttcgatcg ctagcaaact gagcaaaagt
     2341 ttacctgcat catctctttt gtcatccctc cacaggtgca ttatcttcac gccgagctcg
     2401 gtcttattct cacgagatag aaagtagggg gcactttcaa cccatacaag cttctttccg
     2461 ggcaagaacg ggggagtcaa aagagaggct tcaatctggt cccacgccgc tgagcggccg
     2521 tcgaagcgtt cgagattgaa ttctctctgg tcggcgggaa cgacaacatc aataagcaac
     2581 ttgcagccgg actgtacctg cagatcatcg ccgaatagaa gcacgaaggg cggtccatca
     2641 ccctttccaa catcggtgag aaaagagtca aagttgcgtt tcatgctgta gcccagcgcc
     2701 cggacggaaa gattcgggct ctttcgatct ttaagcggga tgaaagtaat gatgaactga
     2761 ccacactaga ggggctaacc aggattccgg tcaatgtttg ggaacccaca tctgattttt
     2821 ctcactccag gcgtaatctg acttccaaac tttgagtccc atcgtgtatt taccgccaga
     2881 tttcgaaacg aaaatccttt cgttcgaata aggaatctca actcgggcga tgatgagtac
     2941 cttttttgag attcccttgt tgatcaaaat acgatcccct acgcgcatcg tcggccgatt
     3001 atctttgcga gctttcatga gcggaaaata gcattgcgga agtgctcagg caaatttctc
     3061 tgagttaatg cgcctatggt ggcctggatc acagagctcc tccaactatg cagctggtgt
     3121 ttacgagcgc gcctacgtac gaccttgacc cccgaaggct ccgagtagct aatttttgtc
     3181 aggacacttg ccttgacaat ctccagcccc tctcctatac ctggttcgat taagtttcga
     3241 ctgggatgat cggtgatcct ttcttaccat a
//
LOCUS       DASUTG010000081         5185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1791313, whole genome shotgun sequence.
ACCESSION   DASUTG010000081 DASUTG010000000
VERSION     DASUTG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791313"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VF089_01760"
     CDS             <1..1006
                     /locus_tag="VF089_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6172711.1"
                     /translation="DEMLDRTSLKQYLESRGAGPLINSVIEQAYKAEYGLEIDEQSCL
                     NFLLFIHADRRSKFTPFGVFSDERYHVISGNDRIVEGLRDRLPGQLQLGMRLLRATKT
                     AAGRIELAFAQGASTKEETFEAVVLALPFTTLREVDLDVSLGLPDSQRDAIRLLGYGS
                     NAKMMIGFNGRPWLSLGSNGEAYSDLPHHQLTWETNPIRASASRGVLTDYSSGRRGAT
                     LNPNDVQNEASLFLQDLENVFAGALASAARSIDGSILAHLEHWPSHPFSKGSYTCYRP
                     GQFTSIAGNEGTPVGNLYFAGEHANSFYEWQGFMEGAALSGIQAAQQILQHIKAGQLG
                     "
     gene            complement(1254..1769)
                     /locus_tag="VF089_01765"
     CDS             complement(1254..1769)
                     /locus_tag="VF089_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX6172712.1"
                     /translation="MWAYTGDQRPPFAETPATGQESVWDYPRPPKLVTDHRRIVVQRG
                     DLLIADSRENYRILETAGPPTFYIPPQNVRLELLMSLPTTSICEWKGAAQYWTIETAT
                     SAREAIAWSYPKAQAPYDTISGYFSFYPGRIECFVDGERVRPQPGYFYGGWITDEIVG
                     PWKGDPGTEWW"
     gene            complement(1969..2568)
                     /locus_tag="VF089_01770"
     CDS             complement(1969..2568)
                     /locus_tag="VF089_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX6172713.1"
                     /translation="MQITDILAQTGGLQSMARELGVSENQAASGAEALIPAILGGFKK
                     QAQSQPSGVEGLGDLLGQLGGGGLMEDVLSPEPTHVDRGNDVLGQIFGSKDVSRTVAQ
                     NAASQSGLEPSLLKKMLPMVAMLVAGYMAKQRSGASPTQASPGGGLGDLLGGMLGGQA
                     SGAGRTMSGAGGGLGSMLDLNGDGNPLDDILRMAGKVLR"
     gene            complement(2605..2997)
                     /locus_tag="VF089_01775"
     CDS             complement(2605..2997)
                     /locus_tag="VF089_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX6172714.1"
                     /translation="MAFFGKILEKLGFGSSAAAAPQQTTAAPASPGAAPTEAAPKPVA
                     EVDVVAQLEQRAAANPQKLNWRTSIVDLLKLLDIDSSFNARKELAAELGCPADLMGDS
                     AKMNMWLHKAVLQRIAANGGNVPKELLD"
     gene            3489..>5185
                     /locus_tag="VF089_01780"
     CDS             3489..>5185
                     /locus_tag="VF089_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6172715.1"
                     /translation="MNIRRTRIVTGLVLFTYLTTHLLNHSLGLISLDTMEAGRAWFLA
                     LWRNPLGTAVFYGSLLTHFTLALYAIYHRRHLRMPFWEALQLTLGLCIPLLLATHFVG
                     TRMAYEWYGIDDMYAKTVLALWRQNPINGLRQASLVLIAWTHGCIGLYFWLRLRPWYP
                     RYAAALYALVLLLPVFALLGFVQAGREVDYLIARDPDWIEKLYHRTNVLRPGEGGDLV
                     NLRDGIIYGFWACLGAVLIARAMRHVFERPGRIQITYPDERQVQVPRGFTVLEASRLA
                     RIPHASVCGGRGRCSTCRIRIIKGLESVPMPSPAELKLLARIGAPANVRLACQLHPTR
                     DISIVPLLPPDANASDGFDRPAYLTGQECEIAVLFADLRSFTSIAERKLPYDVVFILN
                     RYAEIVGAAIERAGGVPNQFTGDGLMALFGVQTGPEQGCQSAVAAAREIVSSVAQLSE
                     MLSEELETALKVGIGIHTGPSVVGQMGYGVGTYLTAVGDTVNVASRLQDLTKEYDCAL
                     VISEEVAQRAGIQMQSSPRHQVNVRNRREPLVVYAISDESRLLAASARQNSHRNSTAG
                     "
BASE COUNT         1002 a         1508 c         1511 g         1164 t
ORIGIN      
        1 ggatgaaatg ctcgatcgca caagcttgaa gcagtatctt gagagccgcg gcgccggtcc
       61 gctgatcaac tcggtcatcg agcaggccta taaagccgaa tacggtttgg aaatcgacga
      121 gcagagttgc ttgaattttc ttttgttcat ccatgccgac aggcgctcga agttcacgcc
      181 attcggcgtt ttcagcgatg agcgctatca cgtcatcagc ggtaatgatc ggattgtcga
      241 aggtctgcgc gatcgtctgc cgggtcagct tcaattggga atgcgactgt tgcgcgcgac
      301 gaaaactgcg gcgggcagga tcgagcttgc cttcgcacaa ggcgcgagca ccaaagagga
      361 aaccttcgaa gccgtggtgt tggcgctgcc gttcacgacg ctgcgggaag tcgatctcga
      421 tgtcagcctt ggcttgccgg attcgcaaag agatgcgatc agattgctgg gttacggcag
      481 caacgcgaaa atgatgatcg gcttcaacgg gcgcccttgg ttaagcttgg gcagcaacgg
      541 tgaagcctat tccgacttac cccatcacca attgacctgg gaaaccaatc cgatcagagc
      601 gagcgccagc cgcggagtgc ttaccgatta ctcgagcggc cgccgcggcg ccactctcaa
      661 tcccaacgat gtccagaatg aagcgagttt gtttcttcag gatcttgaga atgtcttcgc
      721 cggtgcgcta gcgagtgccg cgcgaagcat tgacggtagt atccttgctc acctcgagca
      781 ctggccgtcc catccatttt caaaaggttc ttatacctgc taccgcccgg gacagttcac
      841 ctcaattgcc ggcaatgaag ggacgccggt cggcaacctc tacttcgccg gcgagcatgc
      901 gaattctttt tacgagtggc agggctttat ggaaggcgcg gcgctttccg gaatccaggc
      961 ggcacaacaa atccttcaac acatcaaagc cggccagctc ggttgatttt gcgtctcccg
     1021 gctcgccgtt ctcgatggat gcgatgccgt cgtcttactt gccacgcagt ttgaagcgaa
     1081 cgcgatcaaa tctcgatgat ctcgcagcgc tcgattaaac attttggatc tctcgagcga
     1141 cagacccgat gccttgtctt catcgaggta ttttcttgtg gcgtagcgga tggccgcgca
     1201 agcattgttg cacacggccg tcggcgggtc tgcattcctc ctttaaacgc cggctaccac
     1261 cattccgttc cggggtcacc tttccaaggg ccaacaattt catcggttat ccaaccacca
     1321 tagaaataac cgggctgcgg gcgcacgcgc tcgccatcga cgaagcactc gattcgacca
     1381 ggataaaagg agaagtaccc cgagattgtg tcgtacggag cttgcgcttt tggatagctc
     1441 caggcaatag cctcccgcgc ggatgtggcg gtttcgattg tccagtactg cgccgcgcct
     1501 ttccactcgc aaatcgacgt ggtgggcagc gacattagca attccagccg tacattttgc
     1561 ggcggaatgt agaacgtcgg cggtccggct gtctcgagga tgcgataatt ctcacgggag
     1621 tcggcaatca gtaaatcgcc gcgttggacg acgatccgcc gatgatctgt aactagttta
     1681 ggagggcgcg gataatccca caccgactcc tgtccagtcg ccggtgtctc ggcgaaaggc
     1741 gggcgttgat cgcctgtata ggcccacatg gttttacctg ctgcaggtcg cgctagtagt
     1801 agtgagaact tacgatcgag ccgagagatt tgtgttggac agattggtgt tggagttgcc
     1861 taactgtacg ctagcggtga tgcgccagcg tatagtgcta gggaagccca acaccgtggt
     1921 gtggactaaa aatctagttg acgaccaaat cggtttcgaa ttgtcgcgct agcgcaatac
     1981 cttgccggcc attctcagga tgtcgtcgag cgggttgccg tcgccgttca ggtcgagcat
     2041 cgagccgagt ccgcccccgg cacctgacat cgtcctgcca gctccgctcg cctgcccacc
     2101 cagcataccg ccaagcagat caccgagccc gccgccgggc gatgcctgcg tcggactggc
     2161 gccgcttcgc tgctttgcca tgtagcctgc aacgagcatg gcgaccatcg gcagcatctt
     2221 tttgagcagc gacggctcga gaccggactg cgatgccgcg ttctgggcga ccgtgcggct
     2281 cacgtctttg gatccgaaga tctgaccgag tacgtcgttg ccgcgatcga cgtgcgtcgg
     2341 ctccggcgaa agcacgtcct ccatgagacc gccgccgccg agttgtccga gcaagtcacc
     2401 aaggccttca acgccggacg gctgtgattg cgcctgcttt ttgaaaccgc cgaggattgc
     2461 cgggataagc gcctccgcgc cgctcgccgc ctggttctcg ctgacgccca gctcccgggc
     2521 catcgattga aggccgcccg tctgtgcgag tatatcggtg atctgcatgt gtgtgactcc
     2581 tcagttgatt gttggcgccg ctgctcagtc gagcaattcc ttgggcacat tgccgccgtt
     2641 ggcagcgatg cgctgcagca ctgccttgtg tagccacata ttcatctttg ccgaatctcc
     2701 catcaaatcc gccgggcagc cgagctcggc ggccaactcc ttacgggcgt tgaagctgct
     2761 gtcgatgtcg agaagcttca gcaggtcgac gatggaagtc cgccaattga gcttctgtgg
     2821 attggccgcc gcgcgttgct cgagttgcgc gaccacatcg acttccgcga ctggcttggg
     2881 cgccgcttcc gttggagccg cccccggact tgccggcgcg gctgtggttt gctgaggggc
     2941 agcggccgcc gagctgccga agccgagttt ctcaagtatc ttgccgaaga atgccatagg
     3001 gatctccttt ctgttgttaa caggccgaac tcaaactcag tttttgtgct gttgcactat
     3061 caagtgcata ccatcgtcaa cccgagaaaa acaggctacg tggcgctttt ctaatagccg
     3121 actcgccggc atcgtgctcc ataacaaagc agcatggaat tcaagtgcaa attgacgcag
     3181 ttggaactag cttgccgccg tcgtgggctg tgatctctgc ttacgacgcg acctgctgga
     3241 ttcaacgcgc aaagacgagc gctgaatgat actaactggt cgagcaagca ctatgaagac
     3301 tcagtcggcg agcaagcagc gaatgatccg gctcaacgtc cgagtacgtt tattgttctt
     3361 taaggttgga acctctggaa ctgctggaac gtttggaacc agcttgtctc catatggcac
     3421 cgagccgaat cggtcctccc gggggcgtag acggcacgct tgccgcgtag tacgttggcg
     3481 caactttcat gaacattcgg cgtacgcgga tcgtcacggg cctggtgctg tttacttatc
     3541 tgaccacgca tctgctcaat cattctctcg gattgatttc gctcgacacc atggaagcgg
     3601 ggcgcgcatg gtttctggct ctctggcgca atccgctggg taccgcggtc ttctacggat
     3661 cgctgctgac ccatttcaca ttggcgctct acgcgattta tcacaggcgg cacctgcgca
     3721 tgccattttg ggaggcgctc cagcttacac tgggtttatg cataccgctg ctactggcaa
     3781 ctcattttgt cggcacccgc atggcttacg agtggtacgg catcgacgat atgtacgcca
     3841 agaccgtgtt ggccttgtgg cgtcagaatc cgatcaacgg cctgcgtcaa gcctcgttgg
     3901 tgttaatcgc gtggacgcac ggctgtattg gcctgtactt ctggctcagg ctgcgcccat
     3961 ggtatccacg ctacgccgcc gcgctctatg cgcttgtgtt gctgctgccg gtattcgcgt
     4021 tgctcggctt cgtccaggcc gggcgcgagg ttgattatct catcgcgcgc gatcccgact
     4081 ggatcgagaa gctttatcac cgcacgaacg ttttgcggcc cggcgagggc ggcgatttgg
     4141 tcaatctgcg cgacggaatt atttatggat tttgggcgtg ccttggtgct gtgttaatcg
     4201 cgcgcgcgat gcgtcacgtg tttgaacgcc caggcaggat tcaaatcacc tatcccgacg
     4261 agcgtcaggt gcaggtgccg cgcgggttca cggtgctcga agccagccgt cttgcgcgca
     4321 ttcctcatgc ctcggtgtgc ggcggccggg gtcggtgttc cacctgtaga attcgcatca
     4381 tcaagggatt ggaatctgta cccatgccaa gcccggcaga actgaagttg ctcgcgcgca
     4441 tcggcgcgcc ggccaacgtg cgcttggctt gccagctcca tcccacgcgc gatatttcca
     4501 tcgtgcctct gcttccccca gacgccaatg caagcgatgg ctttgacagg cctgcctatc
     4561 tcacagggca ggaatgtgag atcgcggtgc tgtttgcgga tcttcgttct ttcacctcca
     4621 tagcagaacg taagttgccc tacgatgtgg tattcatcct gaaccgctat gccgaaatcg
     4681 tcggcgccgc tatcgaacgc gcgggcgggg ttcccaacca attcacgggt gatgggctca
     4741 tggctctgtt cggcgtccag actggcccgg agcagggatg ccagtccgcc gttgccgcgg
     4801 ctcgcgagat tgtcagcagc gttgcgcagc tcagcgaaat gctctctgag gaactagaga
     4861 cggctctcaa ggttggcatc ggcattcata cgggaccttc agtggtgggg cagatgggct
     4921 atggcgtggg gacgtatctt acggccgtgg gcgatacggt gaacgtcgca agcaggctgc
     4981 aggatctaac gaaagaatac gactgcgcgc tggttatttc cgaggaagtc gcccaacggg
     5041 ccggaatcca aatgcagagt tcgccccgcc atcaggtcaa cgtcagaaac cgccgcgaac
     5101 cgctggttgt ctatgcgatc agcgatgaga gccgcttgtt agcggcaagc gctcgccaaa
     5161 actcccaccg caatagcacc gccgg
//
LOCUS       DASUTG010000082         5926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_456815, whole genome shotgun sequence.
ACCESSION   DASUTG010000082 DASUTG010000000
VERSION     DASUTG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456815"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF089_01785"
     CDS             complement(<1..184)
                     /locus_tag="VF089_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172716.1"
                     /translation="MAIGIAEVICTVGIPAFSNSVVIAAPLRVLVPQVDVNITASIPS
                     RLALSAISRPMRRVFDS"
     gene            129..335
                     /locus_tag="VF089_01790"
     CDS             129..335
                     /locus_tag="VF089_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172717.1"
                     /translation="MEKAGIPTVQITSAMPIAKMVGCNRLVLGNGIVHVVGDAKLSAE
                     QEKEVRRQLVRNALAALQGDESSK"
     gene            complement(422..1567)
                     /locus_tag="VF089_01795"
     CDS             complement(422..1567)
                     /locus_tag="VF089_01795"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX6172718.1"
                     /translation="MAKPFDLLLTGGTVLNPATNTKQKLDLGVTAERITAIHANLPRA
                     NAKKTLDVTNCYVTPGLIDFHVHSYWGVNPYGFNADPICLASGVTTTMDAGSSGPINF
                     LGFRKLVHEQSRTRMLAFVAMAQHGVLNDPGELENLRFADPEAAAQAVAENRDVAIGI
                     KVRMHKKSVGENSREALRLAVRTGEACRAPLMVHVGDTAISMEEIVGSLRAGDVVTHC
                     YTPQEPSILDKNGRLLDAVRKAQERGVIFDVGHAGGHFDFDLVRRAMGEGLLPDIISS
                     DLHGRLKQPGFGVVGDLPTVMTKFLALGWSLERIIAACTVNPARAVGWQDRLGSLEVG
                     READIAVLQVVAESVKLKDSVGGEMIARQRIAPRWTIRRGEAFGAKG"
     gene            complement(1801..2823)
                     /locus_tag="VF089_01800"
     CDS             complement(1801..2823)
                     /locus_tag="VF089_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172719.1"
                     /translation="MTVIDADAHVIENESTWDHMLESERRFKPRIVGATDGDPSVEYW
                     LIDGRLMPRSNVEKSLPEAARDMSDLSIRIAHMNELNIDLQVIYPTIFIFPTARRPEV
                     DLALCRSYNRWMADIIRKAPERFRWVVVPPLLNMDCVAEELKFGKERGACGVYLRGLE
                     ADRRLSDSYFFPLYDAASKLDLPICVHSANGSIEAYDFFLDEPGFCKFKLAVVGAFHS
                     LLVDGIPEKFPGLRIAIVEVSAQWIPYAVHDLARRYQRRGKQLSSRVLEDHRVYVTCQ
                     TDDDLEYILGYAGENNLVIGTDYGHADNASEIEALRKLRTGGKIGARVIDKILDDNPR
                     ALYAID"
     gene            complement(2925..3665)
                     /locus_tag="VF089_01805"
     CDS             complement(2925..3665)
                     /locus_tag="VF089_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6172720.1"
                     /translation="MLDGKVVIVTGGAQGIGRHAATTFAQQKAKVVIADINEEKARNT
                     ATELAQHTQTLAMGVDVRDEESIKRMIDEVVDSFGQIDVHVNNAAIVPHFAWGIPRWP
                     RISEMPKDFWDRVIQTNLNGTFNGTKHVIPHMEKRRTGHIINLYGGGGITPAGACAYM
                     VTKDGIRTFSRYVAEEVRSSNICVVTFSPRVPIVTEDAPEEAFKRLPGPEILGQGFVL
                     AAQLPMEQSGGCFAYDNGKLVKETAREG"
     gene            4003..4362
                     /locus_tag="VF089_01810"
     CDS             4003..4362
                     /locus_tag="VF089_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172721.1"
                     /translation="MTYRTLAGSMLLVFMASACSGLGGSRRFEGETISQAQATEERAR
                     IIERLVPEDTPISRNRLAAVADATAAVIKDIDRDRVPRVASGVRNVLRDASLQDLRQI
                     ERILPEIERKRKLDGVD"
     gene            complement(4387..>5926)
                     /locus_tag="VF089_01815"
     CDS             complement(4387..>5926)
                     /locus_tag="VF089_01815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172722.1"
                     /translation="GEFGGKGDATDLPIAYFLAQRSSRPVKIVMNYAEELSACNPAHP
                     TVITVRSGVNRDGRILGRSLRAIHTSGAYGGLKANSSLATWHYVGVPYRVENSHFEFL
                     QIYTNTVPGGYYRSPGAVAMAFALESHTDLIAAELGIDPAEFRLKTLLSEGEEDAVGH
                     RLHDVRFREVLERALEAAKWRDPKPRANVGRGISLSARHISGGDTGLVLSAQADGTFL
                     VLSPTIDQGSGTHTILRQLVAEQMNVPVKNVRVVVGNTDTAPRDSGVRASRVTYVAGN
                     AMVKACGELRERLLERAARLLECRVDEIEFSDGRFCLRQDPNQQLTLPRLVSQAKDPL
                     QVAVYEDYPYPDEVSYICAQVAEVEVDPVTGAVRVERMITAHDVGTVINPLTHQGQID
                     GATIMGIGQGIMEELIMNEGKIVNNNLGDYKLPTIADIPMLKTVLVYSDGGVGPINAK
                     PIGEFANNGPPAAIANAIADAVGVRLFELPLAAEKIYYLLRDQTAKTLGGSVNPVAPK
                     PRRR"
BASE COUNT         1306 a         1649 c         1654 g         1317 t
ORIGIN      
        1 cgctgtcgaa tacgcggcgc atggggcgtg agatggccga taaggccaag cggctcggta
       61 tcgacgcggt gatattaacg tctacctgag gtacgagtac tcgcagcggc gctgcgatca
      121 cgactgagtt ggaaaaggcg gggattccca ccgtgcagat tacttcggct atgccgattg
      181 ccaagatggt gggctgcaac cggctcgtgc ttggcaatgg tatcgtccac gttgtcggcg
      241 acgccaagtt atcggcggag caggagaagg aagtgcgccg gcaattggtg cgcaacgcgc
      301 ttgcagcgct tcagggggac gaaagttcaa aatgacatag tgaatcgaga gccctccgga
      361 gtgaggcact tggtttgtga tcaagtatgt tcaaatcgtt cgaacgtttt gaaccttccg
      421 cttagccctt ggccccgaat gcttcgcccc gacgaatcgt ccatcgcggt gcgatccgct
      481 gccgcgcaat catttcgccg ccaacagaat ctttaagctt caccgattca gcaactactt
      541 gaagcaccgc gatatccgct tcgcggccga cctcgagact gccgagacgg tcctgccagc
      601 cgaccgctcg ggctgggttg acagtgcaag cggcgatgat ccgttcgaga ctccatccca
      661 acgctaaaaa cttcgtcatc acggtcggta aatcaccgac gactccgaac cccggctgtt
      721 tcaaccggcc atgcaggtcg gagctgatga tgtcgggcag caaaccctcg cccattgcgc
      781 ggcgcacgag gtcaaaatcg aaatgcccgc cggcatgacc gacgtcgaag attacgcctc
      841 gttcctgagc tttccgcacc gcatcgagaa gccggccgtt cttatctagg atcgatggtt
      901 cttgcggcgt gtaacagtgc gtgacaacat cgccggcgcg cagcgaaccg acaatctcct
      961 ccatggaaat cgcggtgtcg ccgacgtgga ccatgagcgg cgcgcggcag gcttcgcccg
     1021 ttctgacggc cagccgtaag gcctcccgac tgttttcacc gacggatttc ttgtgcatgc
     1081 gcactttgat gccgatggcg acatcgcgat tttcggcgac cgcctgtgcc gctgcctcgg
     1141 gatcggcgaa acgtaagttc tctaactcgc ccggatcgtt cagtacgcca tgctgcgcca
     1201 ttgcgacaaa ggcgagcatg cgagtgcggg attgctcatg gaccagtttg cgaaacccca
     1261 gaaaattaat cggccctgac gaaccggcat ccatggtcgt cgtcacgccg ctggccaggc
     1321 aaattgggtc ggcgttaaag ccataggggt ttacgcccca atagctgtgc acgtgaaaat
     1381 cgatcaggcc cggcgtgacg tagcagttcg tgacatccaa agttttcttg gcgtttgccc
     1441 gcggcaaatt cgcatggatc gccgtgatgc gttcagcggt gacgccgagg tcgagcttct
     1501 gcttggtatt tgtcgccgga ttaagaaccg tgccgccggt tagtaagaga tcaaacggtt
     1561 ttgccattca gcgcctttcg aactgattcc taagattcac cggatagcaa catcaccaag
     1621 atccgaacca aagattcaaa tacttcccct tgacgggaga aggacaaagg aacgggtgat
     1681 ctgtattcga cgagagcacc cgtaaccaac ctttcggcat cacaaggaga aaggctcgga
     1741 cttcaaagtg ggctccgtgc agtgtctgta cgatcacgct tcacatcacg agccgcggtt
     1801 ctaatcgatg gcgtaaagcg cccgtggatt atcgtcgagt attttgtcga tgacccgcgc
     1861 accgatcttc ccccccgtcc gcagcttgcg caacgcttct atctcgctag cgttatccgc
     1921 atggccgtaa tcggtgccga tgacaagatt attctcgccg gcataaccga ggatgtactc
     1981 gagatcatcg tcggtttggc aggtgacata tactcggtga tcctccagga cccgagagct
     2041 gagttgtttg ccgcgccgct ggtagcgacg cgccaaatca tggaccgcgt aagggatcca
     2101 ttgcgcgctg acttcgacaa tggcgatgcg caaaccggga aacttctctg gaatgccatc
     2161 gaccagaagc gaatgaaacg cgccgaccac cgccagcttg aatttgcaga agccgggttc
     2221 atcgaggaaa aagtcgtaag cctcgatgct gccgttggcc gaatgaacgc aaatcggcag
     2281 atcgagtttg ctggccgcgt catatagcgg aaaaaaataa gaatcactca agcgccggtc
     2341 ggcttccaag cctcttagat acaccccgca cgcgccacgc tccttgccga atttcaattc
     2401 ctcagcgaca caatccatat tgagcagagg cgggaccacg acccagcgaa atcgctcggg
     2461 agctttacga ataatatcgg ccatccagcg gttatagctg cggcaaagcg cgaggtcgac
     2521 ttcgggccgc cgcgcggtcg ggaagataaa aatcgttgga tagatcacct ggaggtcgat
     2581 attcaattca ttcatgtgag cgatccgaat gctcagatcg ctcatatcgc gggcggcttc
     2641 gggcaaactt ttttcgacgt tgctgcgcgg catgagcctg ccgtcgatca accagtactc
     2701 cacggatgga tcaccgtcgg ttgcgccaac gatacgcggt ttgaaacgac gctcggattc
     2761 gagcatatga tcccacgttg attcgttctc gatgacgtga gcatccgcat cgatcaccgt
     2821 cattgtttac tcctcatgaa accagcaaaa gtacttacta ttcacgaagc acatagatga
     2881 aactgttaca ctttagacta ggaccgcata gcgattaagc tgcatcagcc ttctctagca
     2941 gtttctttca ccagcttgcc gttatcgtag gcgaagcagc cgccgctttg ttccattggg
     3001 agttgcgcgg cgagaacaaa accttgtccg agaatttcgg gaccgggcaa gcgcttgaac
     3061 gcctcttccg gagcgtcctc ggtcacgatc ggtacccgcg gcgaaaaggt cacgacacat
     3121 atattcgagc ttctgacctc ttcggcgaca tagcgactga aggttcgaat gccatctttg
     3181 gtaaccatgt aggcgcaggc acccgccggt gttattccac cgccgccgta aagattaatg
     3241 atgtgcccgg tccgcctttt ttccatgtgg gggattacat gtttggtgcc gttaaaagtg
     3301 ccgttcaggt tggtttgaat aacccgatcc caaaaatcct tgggcatttc gctgatgcgc
     3361 ggccagcgcg gaatacccca agcgaaatgg ggcacgatgg cggcgttgtt gacgtgcacg
     3421 tcgatctgac caaaactatc cacgacctca tcgatcatgc gtttaatgct ctcttcgtcg
     3481 cgcacgtcga ctcccattgc gagggtttgg gtatgctggg ctagttccgt ggcagtgttc
     3541 cgggcttttt cttcgttgat gtcggcgatg acgacttttg ctttctgctg cgcaaaagtg
     3601 gtcgctgcat gccggccaat gccctgtgcg ccgccggtga cgataactac ttttccatcg
     3661 agcatggtga acctcctgaa actgtgtcta acgcgaaacc gcggatttgc gaggtcggtc
     3721 gccaactgtt tactttcgac tcgtccgaaa taagtcgagt gtcggatgtt gttgtttcaa
     3781 taacgtattt gatcggccag tttattgtca atcagcatgc caatcctcct tttgcggacc
     3841 gtggcaggtg gcgctaagat ttagcagtta gcagaaagcg aggtaaagct cctgtaaggc
     3901 gagtccaaga gaggattata gtttttcgtc tcagaaatct ttgttaggga ctgtgatcgt
     3961 cattcgtgca ggcgagatca cacctccggg agggtttcta acatgaccta tcggacattg
     4021 gcaggttcaa tgttacttgt cttcatggca agtgcttgca gtggattggg tggcagtagg
     4081 cgtttcgaag gtgaaacgat ttcacaagcc caagcgacag aagagcgcgc gcgaatcatc
     4141 gaaaggttgg tgccggaaga cacacctatc tcacggaacc gactcgccgc tgtggctgac
     4201 gcaacggctg cggtaatcaa agacattgac agagaccgtg ttccccgagt ggcgagtggc
     4261 gtgagaaacg tgctgcgtga tgcaagtttg caggacctca gacaaatcga gcgcatcttg
     4321 cccgagatcg aaaggaaaag aaaactagat ggggtcgact aagcctaact gtcgatgctc
     4381 gccaaactag cggcgtcgag gcttaggcgc cactgggtta acggatcccc cgagcgtctt
     4441 cgcggtttga tccctgagta agtaatagat tttttccgca gcgagcggca gctcgaaaag
     4501 gcgaacacct acggcatcgg caatggcgtt ggcgatggcg gcgggcggac cgttgttggc
     4561 aaattcgccg atgggcttgg cattgatcgg gccaacgccg ccgtcggagt acactaagac
     4621 ggttttgagc attggaatgt cggcgatagt agggagcttg tagtcaccga gattattgtt
     4681 gacaatcttg ccctcattca tgatcaactc ttccattatg ccctgtccga tacccataat
     4741 cgtcgcacca tcgatttggc cttgatgggt caacggattg atgaccgtcc cgacatcgtg
     4801 agcggtgatc atccgctcga cgcgtaccgc gccggttaca ggatcgacct caacctcggc
     4861 gacctgggcg cagatataac tcacttcgtc cggataaggg tagtcctcat aaacggcaac
     4921 ctgcaaagga tccttggctt gagaaacgag ccgcggcagg gtcagctgct gatttggatc
     4981 ttgacgcaag caaaatctgc cgtcgctgaa ttcgatctca tccacccggc attccaagag
     5041 acgcgcggct cgctctagga gccgctcgcg cagttcgcca caggccttca ccatcgcgtt
     5101 acccgccaca taggtgacgc gactggctct aacgccactg tcgcgcggcg ccgtatcggt
     5161 attgccgact accacccgca cgtttttaac cgggacattc atctgctcgg ctacgagttg
     5221 acgcaagatg gtatgggtgc ctgagccttg atcgatggtc gggcttaaca ccagaaaagt
     5281 tccgtcggcc tgcgcgctta gaacaagacc tgtgtccccg ccgctgatat gccgtgccga
     5341 tagcgagatg ccgcggccga cgttagcgcg aggttttggg tctcgccatt tggccgcttc
     5401 caacgcccgc tctagcacct cgcgaaagcg aacgtcgtgc aatcgatgac cgacggcgtc
     5461 ctcttcgcct tcgctcagga gagttttcag gcgaaactcg gccggatcga tcccaagctc
     5521 tgcggcgatc agatcagtgt gcgactccag agcaaatgcc atcgccaccg cgccggggct
     5581 gcgatagtat ccgcctggca cagtgttggt gtaaatctgc aaaaattcga aatgggaatt
     5641 ttccacccga tacgggacac cgacatagtg ccaggtcgcc agcgacgagt tggctttgag
     5701 tccgccgtaa gcgccgctag tatggatcgc ccttaacgaa cggccgagaa tacggccgtc
     5761 gcggttcacg ccgcttcgca cggtgattac ggtcggatgc gctggattgc aggccgacaa
     5821 ttcctcggca taattcatga cgatttttac cggccgcgag gacctttgag ctagaaagta
     5881 ggcaatcggc agatcggttg catctccttt gccgccgaat tcgcca
//
LOCUS       DASUTG010000083         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1643317, whole genome shotgun sequence.
ACCESSION   DASUTG010000083 DASUTG010000000
VERSION     DASUTG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643317"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..1365
                     /locus_tag="VF089_01820"
     CDS             361..1365
                     /locus_tag="VF089_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172723.1"
                     /translation="MRNIALSVVLATVTLVVFSPRAHTQVRSSADKVRVVYSAIGSSQ
                     SPLWIAHEAGLFKKHGLDAEILYVAGGSRAAQVVLSGEAPVAMFNGSSVISANLAGAD
                     LVNLASGMNVLPFFLVTGPGVKQVEDLKGGKVAITRFASATDFALRFAAEKWPIKPER
                     DFAVIQLGGQPEMMAALKSGAVQGAMLNAEFTILARREGYRDLVDVASLGLVFPGSGV
                     NTSRSFIKSRRDTVLRIMRAYTEGVHLGRTNKTFGVTVLGKYLKNQDVPFLQAVHELY
                     LGRYIPWPPYPSVEAMKRALEDVAERDPRARAMRPEQFMDVSLMQELDKEGFIKQLGR
                     "
     gene            complement(1459..2046)
                     /locus_tag="VF089_01825"
     CDS             complement(1459..2046)
                     /locus_tag="VF089_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1810..1812),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6172724.1"
                     /translation="MKPLLFVLALLLAFNQAAVSAQTGSKDEVKQPATDLDRVKVGQP
                     APDFTLEDSDGKSVGLADYRGKKNVVLVFYRGHWUPYCVAQLGELQSLLNKQDSGRSQ
                     ILAVSIDSHEDSKKLRQKLQAANNGSQTFPLLQDTDHKVIDRYGLLNPSGKGWPHPTT
                     YVIDRQGVVRWKFTETNYTIRPTNKQILQEIRKLK"
     gene            complement(2088..2894)
                     /locus_tag="VF089_01830"
     CDS             complement(2088..2894)
                     /locus_tag="VF089_01830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6172725.1"
                     /translation="MNFTDQVILITGVSDGIGKRLALDLAARGAVIIGCGRSEERLGA
                     VLTELQCISPRSITIKCDVSSREEVRAMVGKVLAAYDAIDILINNAGIGMRKPFVETP
                     LRIIEELMRTNYLGTVYCTREVLPSMLARGRGHIVNISSVAGHIGTLNMAGYCASKFA
                     INGFSESLYHELKPRGIAVSVVCPGPVKTAFNRSFAQSSPKSPERMIISPESVSQTVM
                     TIIENKRFEMITPLSFALICGVKRLMPGLFRAISSRLFRSHVSPQPKSGT"
     gene            2967..4259
                     /locus_tag="VF089_01835"
     CDS             2967..4259
                     /locus_tag="VF089_01835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX6172726.1"
                     /translation="MSSTNGHVSNGGPPRRRVVVTGIGAISPNGIGREAYWKALRCGR
                     SGVDLISQFPTEELSCQIAGEVKNFRAEDYLPPQDLRRVGRAVPLLLAATREALTDAG
                     IAWEKLSLDDRRSWGVIVGSGGGTPDFTEEQYRLFFSNQMRKVSAYNVSGSTPGNVSS
                     EVSLHFDLRGPSHLISTGCTSSTDALGYAFNMIRFGLAEHLVTGGTDATITPGIMQGF
                     CVMRVVSSSRNSEPQRASRPFDRSRDGFVLGEGAWTLVFEERHSALARGAQIYAEVLG
                     YGSTCDAYHRVRLDPNGEEPARAMSLAIQDAAIAKDEIGYLALHGTSTILNDRTETVA
                     AKLCFGRRAYEIPMSSVKSMIGHPQGASGAAGVVAAIMAMNHSFLPPTINCDEPDPEC
                     DLNYIPNRGLARPVDISLCNCIGFGSKNSALVIARGEL"
     gene            4256..>4619
                     /locus_tag="VF089_01840"
     CDS             4256..>4619
                     /locus_tag="VF089_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6172727.1"
                     /translation="MKRSMAREMMDSPDNPRELLEDDLRNLRIINRYLGNYRAVLGGL
                     ARLIEEQRLQRFSLLDVGTGSADIPAFIASWARRRHLTAQLVGLERDTVTLEAAVNQT
                     RNYPEISLVRGDGRALPLA"
BASE COUNT          981 a         1258 c         1333 g         1047 t
ORIGIN      
        1 caccacggcc caggccaaaa accagtttaa ttgtcgagtg tttttagtgg gtggcattac
       61 cggctgaaga tgatttcatt gcgcgaagac tctgattccg gtcaatcaac ggttttctac
      121 cgcagtgggg atgtcagcac cgacctgata caatgcacca caaatgatct tgatgctcga
      181 atatgtcacc gatggaccac gaggtcatgc actgaacgcc agagatgcat tctcgcggtt
      241 aactcattgc gtacacttct ggcttccgct cgtcaatcca cgtgcttgct tgaggaaccc
      301 ggctgtccca cggaagccgt ctaataatta aatataagta aataattcga agaggataga
      361 atgagaaaca tcgcgttgag tgttgtacta gcgacagtga cattggttgt attctcgccc
      421 cgtgcgcata cgcaggtgcg atcaagcgcc gacaaagtcc gggtggttta tagcgctatc
      481 ggcagctccc agagtccgtt atggattgct catgaggcgg gattatttaa gaagcacggg
      541 ctggatgccg agattcttta cgttgccggc ggctcacggg cggcgcaggt cgtcttatcc
      601 ggcgaggcgc cggttgccat gttcaacggc agctcggtga tcagcgcgaa tctcgccggg
      661 gcggatctgg ttaatctggc cagcggcatg aatgttctgc ctttcttcct ggtcaccgga
      721 cccggcgtca agcaagtcga ggatctcaaa ggcgggaaag ttgcgatcac acgattcgct
      781 tcggcaactg actttgctct gcgttttgcc gcggaaaagt ggccgattaa accggagcgc
      841 gattttgcgg tgatccagct aggcggacag cccgagatga tggccgccct caagagtggc
      901 gcggtgcagg gagccatgct caacgctgaa tttacgattc tcgcgcgtcg cgaaggctat
      961 cgcgatcttg tcgacgtagc ttcgctgggg ttggtttttc cgggttcggg agtcaatacc
     1021 agccgatctt ttattaaaag ccgccgtgat acggtattgc gaatcatgcg cgcatacacg
     1081 gaaggcgttc acctcggcag aaccaacaaa acctttggcg ttaccgtgct gggaaagtat
     1141 ctcaagaatc aggacgtgcc atttttacaa gcggttcacg agctctatct gggccgctat
     1201 attccctggc ctccttaccc ttcagtggaa gccatgaaac gggctctcga ggacgtcgcc
     1261 gagcgagatc cgcgcgcccg ggccatgcgc cccgaacagt tcatggatgt cagccttatg
     1321 caggagctcg acaaggaagg ttttatcaag caactggggc gctgagactt acaagggaaa
     1381 agttcaactg ttcaaaggtt caaacgttca acgccagata gacactttcc gggctaagca
     1441 acagtcgcca cgggccgatt acttgagctt tctgatctct tgcaggattt gtttgttcgt
     1501 tggtcggatt gtgtagtttg tttcggtaaa tttccagcgc actactccct gcctatcgat
     1561 gacatacgta gttggatgag gccagccttt gccactcggg ttgagaagcc catagcgatc
     1621 gatcactttg tgatcggtat cttgaagcag gggaaaggtt tggctgccat tgtttgccgc
     1681 ttgaagtttt tgccgcaatt ttttcgagtc ctcatggctg tcgatgctca ccgcgaggat
     1741 ttgcgatctc ccgctgtctt gtttatttag caggctttgc agctcgccga gctgcgcgac
     1801 gcaatagggt caccagtggc cacggtaaaa aaccaacacg acattttttt tgccgcggta
     1861 atcggcgagg ccgacgctct taccgtctga atcttcaagc gtaaaatccg gcgccggctg
     1921 cccgaccttc acacggtcga gatccgttgc cggctgttta acttcgtcct tcgaacccgt
     1981 ttgggctgag actgcggctt ggttgaaggc taacagcaga gcgagcacga agagcagggg
     2041 cttcatcaga gtttgttcct ccttatctaa ccagcccaaa gcaccgatta tgtcccggat
     2101 ttaggttgcg gcgaaacatg agaacgaaag agccgggacg aaatagcgcg aaatagacca
     2161 ggcatcaagc gcttgacgcc gcagatcaat gcgaatgaga gcggcgtgat catttcaaag
     2221 cgcttgttct cgatgattgt catcaccgtt tgcgagacgg attcagggct gatgatcata
     2281 cgttccggcg acttcggcga actttgggcg aagctccggt tgaatgccgt cttcacggga
     2341 ccgggacaaa caaccgaaac cgcgataccc cgaggcttga gctcgtgata aaggctttca
     2401 ctaaaaccgt tgatggcgaa cttagaagcg caataaccgg ccatgttcag ggtgccaatg
     2461 tggccggcca cggatgagat attgacgata tgaccgcgac ctctcgcaag cattgacggt
     2521 aagacctcgc gggtgcagta aactgttccg agataattcg tccgcataag ctcttcgatt
     2581 attcggagcg gggtttcgac gaagggctta cgcatgccga tgcctgcatt gttaatgaga
     2641 atatcgatgg cgtcgtacgc cgcgagtact ttcccgacca tcgcgcggac ctcttccctt
     2701 gaagagacgt cacacttgat cgtaatcgat cgggggctaa tgcactgaag ctcagtaaga
     2761 acggctccaa ggcgctcctc agagcgcccg caaccaataa tcacggcacc gcgtgctgct
     2821 agatccagtg ccaggcgttt gccgataccg tccgatacgc cagtaatcaa aatgacttga
     2881 tccgtaaagt tcatctgccg tttctaggtt agctcattcg gtagtgttat gctgaaagga
     2941 aactatgtcg gctagaaaga gtcaatatga gttccactaa cggtcacgtg tctaacgggg
     3001 gcccgccgcg gcgccgtgta gtggttaccg ggattggcgc catcagcccg aacggcattg
     3061 ggcgtgaggc ttactggaag gctctgcgct gcggccggag tggggtcgat ttgatctcgc
     3121 agttccctac cgaagagctg tcatgccaaa ttgccggtga agttaaaaac tttcgcgccg
     3181 aggattatct cccgccgcag gatttgcgcc gggtggggcg agccgtgccg ctgctgcttg
     3241 ccgccacgcg agaagccttg acggatgcgg gtattgcttg ggagaagttg agcctcgatg
     3301 acaggcgctc ctggggggtg attgttggca gcggcggggg caccccggat ttcaccgaag
     3361 agcagtaccg cctgttcttt tccaaccaga tgcgcaaggt gagcgcttac aatgtctcgg
     3421 gctcaacgcc aggcaacgtc tccagcgagg tctcgctcca tttcgacctg cgtggcccga
     3481 gtcatctcat ctccactggt tgcaccagct ccaccgacgc gctcggctat gcgttcaaca
     3541 tgattcgctt cggcctggcg gagcacctgg tcacgggcgg caccgatgcg acgatcacgc
     3601 cgggaatcat gcaagggttc tgcgtgatgc gggttgtctc ctcgtcgcgt aacagcgaac
     3661 cgcagcgcgc ttcgcgcccg tttgatcgca gtcgcgacgg tttcgtgctc ggcgaaggcg
     3721 cttggaccct agtcttcgag gagcgccaca gcgctttagc ccgcggcgca caaatctatg
     3781 cggaagttct cggttatggc tcgacctgtg acgcgtatca ccgggtccgg cttgacccga
     3841 atggcgaaga gccggcgcgg gccatgagcc tggcgatcca agacgctgct attgccaagg
     3901 acgagatcgg ttacctggcg ctgcacggca cctcgacgat cctcaacgac aggaccgaaa
     3961 ccgttgccgc caagctttgt tttggccggc gcgcttacga gatccccatg agctcggtga
     4021 aatccatgat cggccatccg cagggagctt ccggcgcagc cggcgtggtt gcggcgatca
     4081 tggccatgaa tcacagtttt ctgccaccga ccatcaactg tgacgagccg gatcccgagt
     4141 gcgaccttaa ctacattcct aatcggggcc ttgcccggcc tgtcgatatt tcgctgtgca
     4201 actgtatcgg cttcggctcg aagaactcag ccctggtaat cgctcgagga gagttgtgaa
     4261 gcgcagcatg gcgcgcgaaa tgatggactc gccggacaac ccacgcgagc tcctggaaga
     4321 tgatctgcgc aatctgcgaa tcatcaatcg atatctcggc aactaccgag ccgtgcttgg
     4381 cggtctggcg cggctgatcg aggagcaaag gctacagcga ttttctcttc tagatgtcgg
     4441 cacgggaagc gccgacattc cggcgttcat cgcctcatgg gcacggcgcc gtcatctcac
     4501 cgctcagctg gtcggtctcg agcgggatac cgtgacctta gaagcagcgg tcaatcagac
     4561 tcgcaattat ccggagatct ccttggtacg aggggatggc cgggcgctgc cgttggctc
//
LOCUS       DASUTG010000084         6616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_309752, whole genome shotgun sequence.
ACCESSION   DASUTG010000084 DASUTG010000000
VERSION     DASUTG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309752"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VF089_01845"
     CDS             <1..769
                     /locus_tag="VF089_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX6172728.1"
                     /translation="HQKIENPRYLYWADRLGLLVWEEMPAAYRFTRTAIKRTVREWTE
                     AIERDYSHPCVVVWVPLNESWGVPDLPSSPEQRHAVEALYHLTKTLDSTRPVIGNDGW
                     ESSATDMIGIHDYDANIEHLRLRYGPEIPVQQLFDRRRPAGRILTLDGYPHRGQPIVL
                     TEFGGIAYDKCRAPGVKTAWGYSVVEDAEEFARRYRELLDVVARMALFSGFCYTQFTD
                     TFQEANGLLCADRTPKIPLRQIRAATETSRSHIAHGV"
     gene            770..1858
                     /gene="galT"
                     /locus_tag="VF089_01850"
     CDS             770..1858
                     /gene="galT"
                     /locus_tag="VF089_01850"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6172729.1"
                     /translation="MHRATFTKPDGRELTLYGRKALSVTKVEAPSPFPDPLHGNPHLR
                     WHPLRGEWITYAAYRQDRTFLPPPKYNPLAVTLDPAHPTELPAGDWDVAVFDNRFPSL
                     SLGAQDPPQLIVPSAPAWGKCEVVVFTQDARSSLGALPLDHLELVLEVWADRTEKLSD
                     LAGIDYVLPFENRGAEVGVTLHHPHGQIYAYPVVPPVPARMQQIALEYYQAHRSGVLQ
                     DLINDELNGPRMLYRGEHAVGFVPVCARYPYEVWVAPITPVASFAHLTGNQRADLARA
                     LKTVLLKFDGLWKRPLPYLMAWYQAPTDGKPHPESHLHAEFYPPYRTPDKLKYLAGTE
                     LAAGFFAMDALPEEKARELQQIQVLLEP"
     gene            1872..2954
                     /gene="galK"
                     /locus_tag="VF089_01855"
     CDS             1872..2954
                     /gene="galK"
                     /locus_tag="VF089_01855"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX6172730.1"
                     /translation="MKTFAEVFGKPPAVSAQAPGRVNLLGEHTDYNDGYVLPTAIPQT
                     TCVSIRENGGKQFSVYAAELEQAAEFTMESAPPEYFASYVYGCVRELHDHSFAAPPLD
                     IHISSSIPIGVGLSSSAALEVATLRALRQLFKLRLDDVTIAQLAQRAEIRYAGVNCGI
                     MDQMASSLADTGSMLFLDTRSLQRRVLPVPARAEILVIDSGVARSLAGSKYNERRAEC
                     EQAAQLLGVRALRDVTDTAAIEKLPQPLRRRARHVVSENDRVLRAVESTDAESFGELM
                     NASHNSLRDDYEVSIRELDRLVELLQGQSGVHGARLTGAGFGGACVALCVEGETRAIG
                     ERVLGAYNNAGQHGRILVPSSGDSRA"
     gene            3124..4101
                     /gene="galE"
                     /locus_tag="VF089_01860"
     CDS             3124..4101
                     /gene="galE"
                     /locus_tag="VF089_01860"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX6172731.1"
                     /translation="MKVLVVGGAGYIGSHMALMLGQQGAQAIVLDDLSSGHADAVLSA
                     RLVQGSIEDTGLLDRLFTDESFDGVMHFASLIQVGESVQHPAKYYRNNVTHTINLLDA
                     MIRHRVRNFIFSSTAAIFGQPEYVPIDEGHPKQPINPYGRSKWMVEQVLQDYDAAYGL
                     KSVCLRYFNAAGADPEARIGETHEPETHLIPLLLQVASGRRESAQVFGDDYDTPDGTC
                     IRDYIHVLDLAAVHSLALAHLTNGGASAAYNLGNGNGFSVRQVIDAVCQVTKKPVPVI
                     DAARRAGDPARLVADATLARKHLGWTPQYPELSTIVSHAWNWERKIAGI"
     gene            4131..5210
                     /locus_tag="VF089_01865"
     CDS             4131..5210
                     /locus_tag="VF089_01865"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX6172732.1"
                     /translation="MAMIAQQIFGQMPDGGNVEFYTLTNDHGLEARIMNYGGTLVSLR
                     APDRNGVQADIVLGFDELSPYLSQHPYFGSLIGRYANRIAGARFTIHGKIYRLSANEG
                     RNHLHGGRIGFDKNLWDAKAEQSSEGPQLVLSHFSRTGEEGYPGNLSVRVTYTLTAKN
                     ELRLDYSASTDQATVINLTHHSYFNLAGAGTIDDHLLRLAAERYLPVDRELIPLGEQR
                     PVRGTPFDFTVPRSIGSGISSDEAQLKLAGGYDHCWVLTQQSAACALAAELYEPISGR
                     ALSVFTTQPGIQVYSGNFLDGGIQGKQGERYQRHSGLCLETQHFPDSPNQPSFPTTML
                     RPGETYREMTVYRFEVDEGIREQKA"
     gene            complement(5263..6489)
                     /locus_tag="VF089_01870"
     CDS             complement(5263..6489)
                     /locus_tag="VF089_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEX6172733.1"
                     /translation="MALLFSPSGDTNLESQDQSLGLNGTAAPKFQSQGPTSKLFDSFW
                     MAGFESSSHISREGVRLDMIDATQHDRFVAEDYRLLRRFGFGTIRDAVRWHLIERGNG
                     YDFSSLAPMVEAAQTESVQIIWDICHYGLPDDVDIFSPAFVNRFERFCGAIASFMKQY
                     SDRIPFYAPINEISFFAWAAGQVGFMHPCRTGEGVALKEQLVRAAIAGIEALWAVDSR
                     ARITQIDPIMHVVTPRQHPELAGAAAAQHASQFEAWDMLSGFVKPELGGHPRYLDIIG
                     VNYYHANQWEYPDDRLRWEDTPRDERWIPFSGLVADVYERYKRPMFVAETGHFGVGRA
                     AWAREIVAEVMLARDSGIPLEGICFYPIIDRTDWEDPSHWHNCGLWDLQRNSDGQLQR
                     VICPEFESELSRIMHR"
BASE COUNT         1442 a         1916 c         1874 g         1384 t
ORIGIN      
        1 gcaccagaag atcgaaaacc cgcgttacct ctactgggct gacagacttg ggctgcttgt
       61 ctgggaggaa atgccggctg cctatcggtt cacgcgaacc gcgatcaaac gcaccgtgcg
      121 cgagtggacc gaagcgatcg agcgcgacta cagccacccg tgcgtcgtcg tctgggtgcc
      181 attgaacgaa tcgtggggcg taccggatct cccttcatca ccggagcaac ggcatgcggt
      241 ggaagctttg taccatctga ccaaaacgct cgacagcact cggccggtga tcggcaacga
      301 cggatgggaa agtagcgcca cggatatgat cggcattcac gattacgacg ctaatatcga
      361 gcaccttcgc ctgcgttacg gaccggagat tccggttcag cagctcttcg accgccggcg
      421 tccagccggg cgaattctca ctctcgacgg ctatcctcat cgcggccagc ccattgtctt
      481 aacggagttt ggcgggattg cttacgataa gtgccgcgca ccgggagtga aaaccgcctg
      541 gggctactcc gtcgtcgagg atgcggagga gtttgcccgc cgttatcgcg agctcttgga
      601 tgtggtggcg cgcatggctc tcttcagtgg cttttgttac acccagttca ccgatacgtt
      661 tcaggaagcc aacggactcc tctgcgccga ccggacgcca aagattccgc tccggcagat
      721 cagggcggcg acggaaactt cgcggagcca catagcccac ggagtctaga tgcatcgagc
      781 gactttcacc aagcctgacg gcagggagct gaccctttac ggacgcaaag cccttagcgt
      841 cacgaaagtt gaagcgccga gcccgtttcc cgatccattg catggcaatc ctcatctgcg
      901 ctggcatcct ttgcgcggcg agtggattac ctatgcggcc taccggcagg accgcacttt
      961 cttgcctccg cctaagtaca atcctctggc tgtgaccctc gacccggcgc atccgacgga
     1021 gctccctgcg ggagactggg atgttgccgt attcgacaat cgctttcctt cgttgagcct
     1081 tggggctcaa gatccgccgc agctgatagt ccccagcgct cccgcttggg ggaaatgtga
     1141 agtggtggta tttacgcaag acgcgcgctc gtcgctaggc gctttgcctc tcgaccattt
     1201 agagttggtc ctggaggttt gggcggaccg aaccgagaag ttgagcgatc ttgccggcat
     1261 cgattacgta ctgccgtttg agaatcgcgg tgcggaggtc ggcgtcacac tgcatcaccc
     1321 gcatggtcag atttatgcct accccgtggt tcctcccgta ccggcgcgga tgcagcaaat
     1381 cgccctggaa tattaccaag cccatcgaag cggcgttttg caagatctca tcaacgacga
     1441 gttgaacggg ccgcggatgc tctatcgcgg cgaacacgcg gtcggtttcg ttccggtatg
     1501 cgcgcgatat ccgtatgagg tgtgggtcgc tccgataacg cccgtcgcaa gctttgctca
     1561 tttgaccgga aaccagcgag ccgatttggc gcgcgcgttg aagacggttc tgttgaagtt
     1621 cgacggtttg tggaaacggc cgcttccgta tttgatggcc tggtaccagg cgcccaccga
     1681 cggcaagccc catccggaat cgcatctcca tgccgagttc tacccgccgt accgcacacc
     1741 ggacaagctc aaatatcttg ccggcaccga gttggctgca ggattttttg cgatggacgc
     1801 actgcccgaa gagaaggcgc gcgagctgca acagatccaa gtacttcttg agccgtgatg
     1861 taagattgct gatgaaaaca tttgccgagg tattcgggaa gcccccggcg gtgagcgccc
     1921 aggcgcctgg ccgggtcaat ctgctgggcg agcacaccga ttacaatgac ggctacgtat
     1981 tacccacggc gattccccag accacctgcg tgtcgatccg ggaaaatggc ggcaagcagt
     2041 tttcggtata cgccgccgag ctcgagcaag ccgccgagtt caccatggag tcagcgccac
     2101 ctgagtattt tgcaagctat gtttacggct gcgtgcgcga gctccatgac cacagtttcg
     2161 cagcgccgcc gctagacatt cacatttcat cgtcgattcc catcggtgtc ggtttgtcgt
     2221 cgagcgccgc cctcgaagtg gctactttaa gggcattgcg ccagctattt aagttgaggt
     2281 tggatgacgt cactatcgct caattggcgc agcgagctga aatccgatac gccggcgtca
     2341 actgcggcat tatggaccag atggcgtcaa gccttgctga taccggaagc atgctgtttc
     2401 tcgacacacg aagcctgcag cgccgggtgt tgccagtgcc tgcgcgcgcc gaaattcttg
     2461 tcatcgactc cggggtcgcc cgctcgctgg ccgggagcaa gtacaacgaa cgtcgcgccg
     2521 agtgcgagca agcggcgcaa ttgctcggcg tcagggctct tcgagacgta accgacactg
     2581 cggcgataga aaaacttccc cagccgctgc gccgccgagc gcgccacgta gtcagcgaaa
     2641 acgatcgggt gctgcgcgcc gtagaatcga ctgatgctga gtcatttggc gagttgatga
     2701 acgcctcgca caacagtttg cgcgacgact acgaagtctc catcagggag ctcgatcgtt
     2761 tggtcgagct cctgcagggt cagtctgggg tgcatggcgc caggctcaca ggggcgggct
     2821 ttggcggcgc ttgcgtggcg ctgtgtgtcg aaggtgaaac tcgcgccata ggcgaaagag
     2881 tcttgggcgc ttacaacaat gcgggtcaac acgggcggat cctcgtgccg tcatccggcg
     2941 acagtcgagc gtgagtctca tgtcgcccca acgacttccc gccaacacgc aaaggcgcca
     3001 aggtaagaaa tcgattccgc gacttggcga cttggcgcga gtaaatcctc gtcataagag
     3061 cgatggacct gaaagaaaat tgtcagatct cgtaatattt cctaagaggt atcaaggaaa
     3121 aagatgaaag tactggttgt gggtggcgca ggatatatcg gctcgcacat ggctctgatg
     3181 ttgggccagc agggcgccca ggcgatcgtc cttgacgatc tctcgtcggg ccatgccgat
     3241 gccgtattgt ctgccaggct ggtgcaaggc agcatcgaag acactggatt gctcgatcgc
     3301 ttgtttaccg atgagagctt cgacggcgtc atgcacttcg cctcacttat tcaagtcggc
     3361 gagtcggttc agcatccggc caagtactac cgcaacaacg tgacgcatac gatcaattta
     3421 ctcgatgcca tgatccggca ccgagtgagg aattttattt tctcttccac tgccgcgatc
     3481 tttggccagc cggaatatgt tccgatcgac gaagggcacc cgaagcagcc gatcaatccc
     3541 tacggccgct ccaaatggat ggtcgaacag gtgctccaag actacgacgc cgcttatggg
     3601 ctcaaatcgg tttgcctgcg ctacttcaac gccgccggag ccgatcccga agcccgcatc
     3661 ggggaaacgc atgaacctga gacccatctc attccgttgc tgttgcaagt tgcctccggg
     3721 cgtcgtgagt cggcgcaggt ctttggcgac gattacgata ctcccgatgg cacctgcatt
     3781 cgagattata ttcatgttct ggatttagcc gcggttcact ccctcgcgct ggcgcatctc
     3841 actaacggcg gtgccagcgc cgcttacaat ctgggcaacg gcaacggctt ctcagtgcgt
     3901 caggtgatcg atgccgtgtg tcaggtgaca aagaagccag tgccggtcat cgatgccgcc
     3961 aggcgcgccg gcgacccggc gaggttggta gccgacgcca ccctggcgcg caaacatctc
     4021 ggctggacac cgcaataccc ggaactctcg acgattgtgt ctcatgcctg gaattgggaa
     4081 agaaaaatcg ccggcatata agcgcagcct caatcaggga ataggccacg atggcgatga
     4141 tcgcgcaaca gatcttcggg cagatgccgg atggcggcaa tgtcgagttt tacaccttga
     4201 caaacgacca cggcctagaa gccaggatca tgaactatgg cggaaccctg gtttcactgc
     4261 gggcgccgga tcgcaacgga gttcaggccg acatagtttt gggtttcgac gagctctcgc
     4321 cttatctctc gcagcatccc tactttggat ctttgatcgg ccggtacgcc aatcgaattg
     4381 ccggcgcgag attcacgatc cacggcaaaa tctaccgcct ctcggccaac gaagggcgca
     4441 accacctgca cggcggacgc atcggcttcg ataaaaattt gtgggacgca aaagctgaac
     4501 aatcatctga ggggccgcag ctcgttctaa gccatttcag ccgaaccgga gaggaaggct
     4561 atcccggcaa cttgagcgta agagttactt acacgttaac ggcgaagaac gagctgcgtc
     4621 tcgattatag cgccagcacg gatcaggcaa cggtcatcaa tctgactcat catagctatt
     4681 tcaatctagc cggcgctgga acaatcgatg atcacctgct gcgcctggct gccgagcgct
     4741 atctgcctgt cgatcgggag ttgatcccgc tcggcgagca acgccccgtc cgagggacgc
     4801 ctttcgactt cacggttccg cgctcgatcg gatcgggaat cagctcggat gaagcgcaat
     4861 tgaagctagc cggcggttac gatcactgct gggttctgac gcagcaatcg gctgcttgcg
     4921 cgctcgccgc ggaactgtat gagccgatca gcggccgagc gcttagcgtg tttaccacgc
     4981 aaccgggaat tcaggtttat agcggcaact ttttggatgg cggcattcaa ggcaaacaag
     5041 gcgagcgcta tcagcggcat tcgggcttgt gcctggagac gcagcatttt ccagactcgc
     5101 cgaaccagcc cagttttcca acgacgatgt taaggcctgg cgagacgtat cgagagatga
     5161 cggtctaccg attcgaagtt gatgaaggca ttcgtgaaca gaaagcttga gccaaccgtc
     5221 tttggatagg gaggcgctaa gctatgctgc agctaatgcg atttatcggt gcatgatacg
     5281 tgacaactcg ctctcaaact ccgggcaaat cacccgctgc aactgaccgt cagaattgcg
     5341 ctgcagatcc caaagaccgc aattatgcca atgactggga tcttcccaat ccgttctatc
     5401 gatgatcggg taaaagcata ttccttccaa cgggatgccg gaatctctag cgagcataac
     5461 ctcagcaaca atttctcggg cccaagcggc gcgtccgacg ccgaaatggc ctgtctcggc
     5521 tacaaacatc ggccgcttgt aacgctcgta cacgtccgcc accaaaccgg agaagggaat
     5581 ccaacgctcg tctcttggtg tatcttccca gcgcaaccgg tcgtccggat attcccactg
     5641 gttggcgtga tagtaattca ctccaattat atcgagataa cgcggatgcc ccccaagttc
     5701 gggcttgacg aatccggaca gcatgtccca tgcctcaaac tggctggcat gttgtgcggc
     5761 cgctgccccg gctagttcgg gatgttggcg cggagtgaca acgtgcatga tgggatcgat
     5821 ttgtgtgatc cgagcgcggc tgtcaactgc ccaaagagct tcgatgccgg cgatggcagc
     5881 ccgcactaac tgctctttca gcgccacccc ttctcccgtc cgacatggat gcatgaaccc
     5941 tacttgcccg gcggcccatg caaaaaatga aatctcgttg atcggtgcat agaagggaat
     6001 gcggtcgctg tattgcttca tgaaactagc gattgcaccg caaaatcgct caaatcgatt
     6061 gacaaacgcc ggcgaaaaga tgtccacgtc atcgggcaaa ccataatgac aaatgtccca
     6121 aattatttga acagactccg tttgcgcggc ttccaccatg ggcgccaacg aagaaaaatc
     6181 ataaccgttg ccgcgttcaa tcaaatgcca gcgcacagca tcgcggattg tgccaaatcc
     6241 aaaccgtcgg agcagacgat agtcttcggc cacgaatcga tcgtgttgcg tcgcgtcgat
     6301 catatccagg cggacgccct ctcgactaat atgcgaggag gattcaaaac cagccatcca
     6361 gaacgaatcg aaaagcttgg aagtaggccc ctgcgactga aacttgggcg ccgcggtgcc
     6421 gttcagcccg agcgattgat cttgtgactc taaattggtg tcaccacttg gagaaaacaa
     6481 aagagccata ggacctttct cccgaccggg cgttggtcct acgacgacca atgctctctc
     6541 gggcacatca acagaatcag ggtaaacggg ctattatact cgcggcgcta gtgacatggc
     6601 tatatttttc tgctgg
//
LOCUS       DASUTG010000085         9799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_754491, whole genome shotgun sequence.
ACCESSION   DASUTG010000085 DASUTG010000000
VERSION     DASUTG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754491"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VF089_01875"
     CDS             <1..821
                     /locus_tag="VF089_01875"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172734.1"
                     /translation="EGIDLEIIFMPANLASTAVLNGDVDYNGAVTGTIGAAVRDQPMK
                     VLLFTVSKPLLFLVGQKNIKDVKQLKGKKIAGSSPGGSATLLANQALKQIGLEPGKDV
                     SVLQMSGNAASRFAVLESGVVDASLLSVPENIVALEKGYNELLFLGDIVEFAQNGFGT
                     SQKRIRENPDEVYRMVRATVRGLQFIWDKNNNDAVTNILMKQWNVTDRKMAAEMFRHV
                     SRVLTKDAYVRPESVQVLIDLARENAKVTKPIPVSDVVDYSFLNKARKELGMTK"
     gene            complement(839..1921)
                     /locus_tag="VF089_01880"
     CDS             complement(839..1921)
                     /locus_tag="VF089_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6172735.1"
                     /translation="MSSSHKDLNTSRQEPLRSSMWVAMYYNNQDVRLQQMRVPRIAAG
                     ELLVRIRASGICGSDLMEWYRIKKAPLVLGHEITGEIVEVGDSVKNFRVGDRVFSSHH
                     VPCGECRYCHSGHQSVCEMLRTTHFEPGGFSEFVRVPRINVDLGTFLLPDEVSFDEGS
                     FIEPLACVVRAQRFARLEAGQTVLVIGSGISGLLHIQLARARGANRIIATDVSDFRLK
                     AAAQFGAQATIHGAEQVPDRIRELNEGRLADLVIVCTGATPAIQQAIKSVDRGGTLLF
                     FAPTAAGVDVPIPLFDFWRDEISIVTSYAGSGDDLIESLELIRGHRVRVGEMITHRLP
                     LSQADLGFRLTASGSDSLKVILDPSI"
     gene            complement(2006..2809)
                     /gene="lsrF"
                     /locus_tag="VF089_01885"
     CDS             complement(2006..2809)
                     /gene="lsrF"
                     /locus_tag="VF089_01885"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEX6172736.1"
                     /translation="MNWGMENRLARIIRPADKRAVMLAVDHGYFLGPTSGLEDPRQTI
                     EPLMPHADSIMLTRGVLRTSVDAKSDKPIVLRVSGGASILKELSNEDITVSIDDCIRL
                     NVSAMALSIFVGSEFEKETLVSLAKLVDEGEKYGIPVLAVTAVGKDMARDVRYLGLAC
                     RIAAELGAHIVKTYYCDDFEQLIESCPIPVIVAGGKKTPELEALELTYNSVKGGAVGV
                     DMGRNIFQSDCPVGMIQAVGAIVHDNVSVKEAYKIYESAKGGIAKRAAG"
     gene            complement(2906..3769)
                     /locus_tag="VF089_01890"
     CDS             complement(2906..3769)
                     /locus_tag="VF089_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6172737.1"
                     /translation="MVTLSATDSQCGVERGRILIFLAALLWSLAGVFIKLLEIHPLTI
                     VFYRSFFASLVFVPFLKFSQFHFNAAIIISVISYTAAITAFVSANKLTTAANAIVLQY
                     TAPVLVYLFSRLVLGEKISTRNALALTVSMVGVGFISFDNAGEPEMSGVLLALLSGVL
                     FAIYMVNLQQIKIISAIYLTWLNNLVCALLVLPFVKAPFALSTDQLLILAIMGAVQLG
                     LPYFLFSRGLGTIPLQEAALIALIEPVLNPVWVALIVGEIPSFATLTGGALILTALAV
                     RYLIPLLSRSG"
     gene            complement(3910..4356)
                     /gene="rplI"
                     /locus_tag="VF089_01895"
     CDS             complement(3910..4356)
                     /gene="rplI"
                     /locus_tag="VF089_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6172738.1"
                     /translation="MEVILKEDVANVGKIGEVVRVRDGYARNYLLPRGLVLLANKKNL
                     KTFEHHKKVVADQKQKITREAQSVGQSISAVSLTIRMRVGEEGKLFGSVTNIQIEKAL
                     KAQGLIVDRRKIQLEEPIKVVGDYEVPIRMTADLTVPLKVSVIPEE"
     gene            complement(4368..5321)
                     /locus_tag="VF089_01900"
     CDS             complement(4368..5321)
                     /locus_tag="VF089_01900"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX6172739.1"
                     /translation="MQRLEIIARLLLATVSTVMLFLGGVTVPPLAFALFPLVPQPVLS
                     FGIRYGVTRGMSVAALALAILLLWGGAQLGYVYSIFVLLIGLVFLSLGRIRVLETLVL
                     GIAAVVFTAFSALLFILYGSWQAIFHDVSESLNSSLMSAVEMHEKMGFPQESIAVLKE
                     RTPEIAQTILHLLPAAAFVSLGLIVLFNVVLLCRRFSEKRPDWLTLVNLREWKAPDFL
                     VWGVIASGFALFIPGLETVKTVAANLLLVFSACYFFQGLAIIAFYFNKNNVPRFVRGV
                     VYLFIVFQQIFTMIVVGLGLFDLWVDFRRLRKQDLNPSQAS"
     gene            complement(5341..5556)
                     /gene="rpsR"
                     /locus_tag="VF089_01905"
     CDS             complement(5341..5556)
                     /gene="rpsR"
                     /locus_tag="VF089_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6172740.1"
                     /translation="MFRRKVCRFCADKTLRIDYKDVRTIASFVTERGKMTPSRITGNC
                     ARHQRLLTTAIKRARSVALLPFTTAKS"
     gene            complement(5846..6229)
                     /gene="rpsF"
                     /locus_tag="VF089_01910"
     CDS             complement(5846..6229)
                     /gene="rpsF"
                     /locus_tag="VF089_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6172741.1"
                     /translation="MTVTNYETLFIVHPEKGPRVKEFIERFRKVIEGQEGVVAQVEEW
                     GLRDLAYRIQKQGKGYYTLFQYRASGRAVEELERNLRLTDGILRYLTVRDDEANEAAA
                     QTPAVDAPEQGDKPPATDATTESPV"
     gene            complement(6301..6867)
                     /gene="pth"
                     /locus_tag="VF089_01915"
     CDS             complement(6301..6867)
                     /gene="pth"
                     /locus_tag="VF089_01915"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6172742.1"
                     /translation="MKVIVGLGNPGRKYDQTRHNIGFRVIDDIAEQNGVGIKKRSCDS
                     LVGELIIMGERVLLAKPQTYMNLSGHAVKALLKHTRSSPENLVVVYDELDLPFGRIRV
                     RQKGSAAGHRGAMSIMETLAGAPFYRVRVGIGRPPQGVEPADFVLKPFSPDEVERLDV
                     VVARAANAALCILQEGGQRAMEKYNRVD"
     gene            complement(6906..7547)
                     /locus_tag="VF089_01920"
     CDS             complement(6906..7547)
                     /locus_tag="VF089_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6172743.1"
                     /translation="METLDIEVDAREPGSKRQARRLRREGKIPAVLYGPKVQPLALEL
                     NRKEFSNRVAGLEGSHLVRLKSASTALADKVALVKEMQYHPITGDVIHADFYEVDLTA
                     RIEVSVPLHFIGKGAGVVRGGILQPIVREIQVECLPLDIPQFFNVDVSALDIGDSVHI
                     EDLPMPEGVTAVYDSNFALVTIATPTVEEEPTAATATAEAATVTSTEEKKEQE"
     gene            complement(7572..8525)
                     /locus_tag="VF089_01925"
     CDS             complement(7572..8525)
                     /locus_tag="VF089_01925"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX6172744.1"
                     /translation="MNQQNTDIKIFAGNSNPPLAREISNYLAVPLGKATVETFSDGES
                     KVEIKENVRGADVFVLQSTSAPGNHHLMELLLMLDAFKRASAKRITAVMPYYGYARQD
                     RKVLPRVPISAKLVADLITTAGASRMLTLDLHSGQIQGFFDIPVDNLYATPVLLQHLK
                     RQLNHNEVTIISPDAGGVERARAIATRLDASLAIIDKRRVGPNVVAEMKIIGEVQDQV
                     AILVDDMVDTAGTLTMAADALKKEGAKRIIGCCTHAVLSGPAIQRIQDSPLEELIVTN
                     TIPLQSEATRCGKMKVLSVAHLIGEAIRRTHEEESISSLFV"
     gene            complement(8708..8781)
                     /locus_tag="VF089_01930"
     tRNA            complement(8708..8781)
                     /locus_tag="VF089_01930"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8747..8749),aa:Gln,seq:ttg)
     gene            complement(8833..9120)
                     /gene="spoVG"
                     /locus_tag="VF089_01935"
     CDS             complement(8833..9120)
                     /gene="spoVG"
                     /locus_tag="VF089_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX6172745.1"
                     /translation="MQVTEVRVFPVDEDKLRAYVTITLDHCFVIRDLKIIKGTTGYFV
                     SMPSKKRKDGTYKDIAHPINNETRRMLEEKVIAEYEKVAAEGGSQRALGNG"
     gene            complement(9196..>9799)
                     /gene="ispE"
                     /locus_tag="VF089_01940"
     CDS             complement(9196..>9799)
                     /gene="ispE"
                     /locus_tag="VF089_01940"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX6172746.1"
                     /translation="ERVEIHIRKRIPIGAGLGGGSSDAAATLIAMNSLFGLNYSTRRL
                     KKRAYGLGADVPFFIDCRPARVRGIGERLTPIKSLPWEWAVIVYPGFPVATAWVYRKF
                     AAKLTKLMANTSIISPLRKSYGQQRFLVNDLESVTVARYPRIKSLKRELIAEGAAGAL
                     MSGSGSAVFGLFDSRGNAARALSRLQKEDGVDTFLVRLLI"
BASE COUNT         2481 a         2729 c         2309 g         2280 t
ORIGIN      
        1 atgaagggat cgatctggaa atcatcttta tgccggcaaa cctggcaagc accgcagtgt
       61 tgaatggaga cgtcgattac aacggcgccg tgaccggcac gatcggcgcg gcggttcggg
      121 atcagccgat gaaggttttg ctcttcacgg tctccaagcc actgttgttt ctggtcggcc
      181 aaaaaaacat taaagacgtt aaacagttaa aaggtaagaa aatcgccggc agttcgccgg
      241 gaggctcggc gacactcctc gccaatcaag cgctgaaaca gatcggcctt gagcctggta
      301 aagacgtttc agttttgcag atgtcgggaa atgccgccag ccgctttgcc gttctggaat
      361 ccggcgttgt cgatgcctcg ttgctttcgg tcccggaaaa cattgtggcg ctggagaaag
      421 gatataatga gttgctcttt cttggcgata tcgtcgagtt tgcccagaac ggcttcggca
      481 cttcgcaaaa gcggatccgt gagaaccccg acgaggtcta tcgtatggtg cgcgccacgg
      541 tacgcggcct gcaatttatc tgggacaaaa ataataacga tgcggtaacc aatattctga
      601 tgaagcaatg gaatgtaacc gataggaaaa tggcggcaga aatgttccgg cacgtaagcc
      661 gggtgctaac caaagacgct tacgtccggc cggaatccgt tcaagtgctg attgacctcg
      721 cgcgcgaaaa cgccaaggta acgaagccga tccccgtttc cgatgtcgtg gattattctt
      781 ttctaaacaa agcccgcaaa gaactaggaa tgacgaaatg accagaggca cgcacctttt
      841 aaatcgaagg gtcgaggatc actttgagcg agtccgagcc gctggcggtg agtcgaaagc
      901 ctaaatccgc ctgcgacaat ggcagtcgat gtgtaatcat ctctccaacc cgcactcggt
      961 gacctcggat aagttcgagc gattcaatca aatcatcgcc actgccggca tacgaagtga
     1021 cgatggatat ctcgtctcgc caaaagtcaa acaagggtat cggcacatca actccggctg
     1081 ccgttggcgc aaagaatagc agcgtgccgc cacgatcgac agattttatc gcctgttgaa
     1141 tggccggcgt ggcgccagtg cagacaatta ccagatcagc taaccggcct tcgttcagct
     1201 cacgtattcg atccggcacc tgctcggcgc cgtgaatcgt tgcctgagct ccaaactgcg
     1261 ccgctgcctt caaccgaaaa tcactaacgt cggtggctat tattcggttg gcgccgcgag
     1321 cacgggcaag ttggatgtgc aacaaaccag aaatcccgct gccgatcacc agcacagttt
     1381 ggccggcttc aagtcgagca aagcgttgcg cacgaaccac gcaggcgagc ggttcgataa
     1441 acgatccttc atcgaaactt acttcgtcag ggagaagaaa cgtccccaga tcgacattga
     1501 tcctaggcac gcgaacgaac tcagagaagc cgcccggttc gaaatgcgtg gtgcggagca
     1561 tttcgcatac tgattgatga cccgaatgac aatagcggca ctccccgcac ggcacatgat
     1621 gagaggagaa gacccgatct ccaacacgga agtttttaac actgtcgccg acttcgacga
     1681 tttcgccggt aatctcgtgc cccagcacca gcggcgcctt cttgatgcgg taccattcca
     1741 tcagatcgct gccgcaaatg ccgctagcgc ggatgcgcac cagcaactca ccggctgcaa
     1801 tccgcggaac acgcatctgt tgaagccgca cgtcctgatt attgtaatac atggccaccc
     1861 acatgctgct gcggagaggt tcctgcctag aggtgttcaa gtccttgtgt gatgaggaca
     1921 tacgatctca aaccaacagc tcggatgctc gatgcggcgc tcaatctccg ggtagttcag
     1981 atgcgcagca ctcaggcagc tattttcagc cggccgcccg cttcgcgatc cctcctttgg
     2041 cactctcata aatcttgtat gcttccttca cagagacatt atcatgtacg atcgccccga
     2101 ctgcctggat catccccacc gggcaatcgg attggaaaat gtttcgcccc atgtcgactc
     2161 caacggcgcc gcctttgacc gagttatagg tcagttccaa agcttccagc tccggcgtct
     2221 tcttgccacc ggccacgatc actgggattg ggcagctttc gatcaactgc tcgaaatcat
     2281 cgcagtagta ggtttttacg atgtgagcgc caagctccgc ggcaatgcgg caggccaagc
     2341 ctaagtagcg aacgtcgcgc gccatgtcct tgcccaccgc tgtcacagcg agcaccggga
     2401 tgccatactt ctctccctca tccaccagct tcgctagact caccagcgtc tctttctcga
     2461 actccgatcc gacgaagatg gaaagcgcca tcgccgacac gttcagacga atgcaatcgt
     2521 cgatggatac cgtaatgtct tcgttggaaa gttctttaag gatgctggcg ccgccggaaa
     2581 cccggagaac gataggctta tcggacttgg catcgacgga cgttctaagc acgccacgag
     2641 tcaacatgat tgagtcggcg tgcggcatga gcggttcaat agtttgccgt ggatcttcca
     2701 agccgctcgt aggaccgaga aaataaccat gatctacggc gagcatgacc gcccgcttat
     2761 ccgccggcct gatgatccga gctaatcgat tctccattcc ccaattcata aatcccatcc
     2821 tccttgcgtt cagaattcaa agttaaccaa ccgtatcaca gaacaatttg ggatctaagc
     2881 cggagccgtc aaaaactttg acttgttaac cggatctact tagaagcgga atcagataac
     2941 gcactgccag agccgtgaga atcaaggcac ccccagtcag ggttgcgaat gacgggattt
     3001 caccaacgat cagagccacc cacacagggt tcagtaccgg ttcgatcaat gcgatcagcg
     3061 ccgcttcctg cagtggaatt gtccccaacc ctctggaaaa aagaaaataa gggagtccca
     3121 gctgcacggc tcccatgatc gccagaatca gaagttgatc agttgaaagc gcgaacggcg
     3181 ctttcacgaa cggcaatacc agcagtgcgc agacgaggtt gttcaaccag gtcagataaa
     3241 ttgcgctgat tattttaatt tgctgcaggt tcaccatgta aatggcaaac agaacgccgc
     3301 tcaacagagc tagcaaaacc ccagacatct cgggctcgcc ggcattatca aacgaaataa
     3361 atccgacacc gaccatgcta accgttaatg ccaaggcgtt gcgagtcgag atcttctcgc
     3421 ccaggaccaa acgagaaaat agataaacca gaactggcgc cgtatattgt agaacgatcg
     3481 cgttggcagc cgtagtcaac ttatttgcgg acacaaacgc ggtaatcgcg gccgtatagc
     3541 tgatgactga gatgatgatc gccgcgttga agtgaaattg cgagaacttc agaaaaggaa
     3601 cgaaaaccag tgaagcgaag aaactccggt agaaaacaat cgtcaatggg tggatctcaa
     3661 ggagcttgat gaaaactccg gccaggctcc agagcaaagc ggcaagaaaa atcagaatcc
     3721 ggccgcgctc tacgccgcat tgggagtccg tggctgacag agtcaccatc ttcagccacg
     3781 actcaaagtc gcggggctac cgctgcggct gaggacttca acaactcaga ttggatggat
     3841 ctgaccgggg gaaaccagga gcgcacgatg ttaacctgta tcagcttgag cgcgattgcg
     3901 cacacacctc tattcttccg gtatcacgga taccttaagc ggcacggtaa gatcggcggt
     3961 catacggatg ggcacctcat agtcgccaac gaccttaatc ggttcttcta attggatctt
     4021 acgcctgtct acgataagac cttgcgcctt taaagctttt tcaatctgga tgttggtcac
     4081 cgaaccgaag agcttgccct cctcgccgac tcgcatgcgg atggtcagcg aaacggcgct
     4141 aatgctctgt cccaccgact gcgcctctct cgtgatcttt tgtttttgat cagcgacaac
     4201 tttcttgtga tgctcgaaag tctttagatt cttcttattg gccagtagga ccaggccccg
     4261 aggcagaagg tagtttctcg cataaccgtc acgaacacgc accacttcac cgattttgcc
     4321 gacattggcg acatcttcct ttaagatcac ttccatagtt ccccccctta cgatgcctga
     4381 cttggattca aatcctgctt tctcagccgg cgaaaatcga cccacaaatc gaataatcca
     4441 agcccgacca ctatcattgt gaatatctgc tgaaaaacga taaacaagta aaccaccccg
     4501 cgaacaaatc ggggcacgtt atttttgtta aaataaaacg cgataattgc cagcccttga
     4561 aaaaagtaac acgcgctgaa aaccagcagt agattggcgg ccacagtctt aaccgtttcc
     4621 aatccgggga taaacagagc gaacccagag gcgatcacac cccaaaccag aaagtcaggc
     4681 gctttccact ctcggagatt cacgagcgtc agccaatccg gtcttttttc ggaaaatcga
     4741 cggcataaca aaactacatt gaataagact atcaggccga gactgacgaa agctgccgct
     4801 ggtaaaaggt ggagaatcgt ctgggcgatt tccggagtgc gttccttcag aaccgcaatg
     4861 ctttcctgtg gaaaacccat tttttcgtgc atctcgacag cagacatgag gctactattc
     4921 agactttcac tcacgtcgtg aaagatcgct tgccaggagc catatagaat gaataacaga
     4981 gcgctgaagg cggtgaacac tacggcggca attcccaaga ctagcgtttc gagtactcgg
     5041 atgcggccaa gtgatagaaa tactagcccg atcagcagaa caaagattga ataaacatac
     5101 ccgagttgcg ctccacccca taacagcaga atcgcgagag cgagcgccgc gacgctcatc
     5161 ccgcgggtaa cgccgtatct gattccaaac gagagtacgg gttgcggcac taatgggaag
     5221 agcgcgaatg cgagcggtgg aactgtcacg ccacccaaga aaagcattac ggtggaaacg
     5281 gtagctagaa ggagcctggc gatgatctct agccgctgca tcgaattaaa atcgtttgct
     5341 ttagcttttt gcagtcgtaa agggcaacaa cgccacactg cgcgctcgct tgattgccgt
     5401 tgttagcagt ctctggtgac gagcacagtt tcctgtgatg cgactgggag tcattttccc
     5461 gcgttccgtt acgaatgagg caatggtccg aacatcctta taatcaatcc tgagtgtctt
     5521 gtccgcacag aagcgacata cttttcgccg aaacatcggc cgacgacgtg aaccaccgcc
     5581 tttgtcgtca tcaatgcgat cgcggttgac tcttgcgccg tattccctct gagccataag
     5641 ctatgttacc tcattccttt ttagattctt actcttacaa tccaggccaa ttagtttccg
     5701 gttagccgcg gcatgtagaa ctcagaagcc tgctacaatc gtcctacttt ggccaagtca
     5761 cctcgaggag cgtatggcga tggctcgaaa agttatcgct gaaatgcaga taagttgtac
     5821 tcggccacgg gcacaatcaa atgaatcata ccggagattc tgtcgttgca tctgtcgcag
     5881 gcggtttatc gccctgctcc ggcgcatcca ctgcaggagt ttgagcggca gcttcattag
     5941 cttcatcatc gcgcacggtt aagtaacgca agataccgtc ggtgagccgc agattacgtt
     6001 ctaattcttc caccgcccgt ccagatgcac gatactgaaa caaggtataa tagcctttcc
     6061 cctgtttctg aatgcggtat gcaagatctc gcaatcccca ttcttcgact tgagcgacga
     6121 ctccctcttg tccttcaata acttttctaa atctctcaat gaattcctta acccgcggtc
     6181 ccttttccgg atggactata aaaagggttt catagtttgt cacagtcatg tttcccttca
     6241 ataataaagc ttctaatcag ctgcgtttcc tctcgattga ggtaacttta gccggaatct
     6301 ttagtcgact ctgttatatt tctccattgc gcgctgccca ccttcttgaa gaatacacaa
     6361 agctgcattc gcggcacgcg caacaaccac atccaatcgc tcgacttcgt ccggtgagaa
     6421 aggtttcaac acaaaatccg ctggttcaac gccctgcgga ggccgcccta tcccgacgcg
     6481 aacgcggtag aatggcgccc cggccagagt ttccatgatc gacatggcgc cccgatggcc
     6541 agcagcactc cccttttgtc gaacgcggat acgaccaaac ggtaaatcca actcatcgta
     6601 aacaaccacc aggttttcgg gtgaagatcg agtgtgtttg agcagcgctt tgacagcatg
     6661 gccacttaga ttcatgtaag tctgaggctt ggcaagtaat acgcgttcac ccatgatgat
     6721 caactcaccc accaacgaat cacaagacct ttttttgatc ccgacaccgt tctgttcagc
     6781 aatatcatcg ataacgcgaa atcctatatt gtgccgggtc tgatcgtact tcctacccgg
     6841 attaccgagc cctactatca ccttcacacg tcggctttaa tccgtgaaaa tctgccagat
     6901 agcgatcact cctgttcctt tttttcctcc gtagacgtca ccgtcgcagc ttccgctgta
     6961 gcggtagccg ccgtgggctc ctcctccaca gttggtgtcg ctatcgttac tagagcaaaa
     7021 ttcgagtcgt aaacagccgt gactccctcc ggcatcggca aatcttctat atgaacggag
     7081 tctccgatat ccagcgcact gacatctacg ttgaagaact gaggaatatc cagcggcaaa
     7141 cattctactt gaatctcgcg cacgataggt tgaagtatcc ctcctcggac aacacccgcg
     7201 cctttgccga tgaagtgaag cggcacactc acttcaattc tagctgtgag atcgacctca
     7261 taaaagtcag cgtgaatcac atcccccgta attgggtgat actgcatctc ctttaccaga
     7321 gcgaccttgt cagcaagtgc ggtggaagcc gatttcaacc gaactaaatg cgaaccctcc
     7381 agtccggcaa ctcgattcga gaactctttt cgatttaact ccaaagcaag tggttgaact
     7441 ttcggtccat aaagaacggc aggaatcttt ccttcgcgcc gcagccgccg agcctggcgc
     7501 ttgcttcccg gttcacgggc atcaacttca atatctaagg tttccaagaa accgacctcc
     7561 tgttaatccg gctacacaaa aagagaacta atagattctt cctcgtgcgt gcgccggatc
     7621 gcttctccaa tgagatgcgc gacggaaaga accttcatct tgccgcaacg agttgcttca
     7681 gattgcaaag gaatagtatt ggtcacgatt agctcctcta agggagaatc ctgaatccgt
     7741 tggatcgctg ggccggaaag aaccgcatga gtgcaacaac cgatgattct cttggcacct
     7801 tcttttttca gcgcgtctgc agccatcgtc aatgttcccg cagtatcgac catatcatca
     7861 actaaaatcg ccacctgatc ctgcacttca ccgatgatct tcatttcggc gacaacgttg
     7921 ggcccgaccc gccgcttatc gataatcgcc aacgatgcgt ccagccgggt tgcgatcgcc
     7981 cgagcccgct caacgccgcc cgcgtcagga gaaatgatcg tcacctcgtt gtggttcaac
     8041 tgccttttaa gatgctggag taatactggc gtcgcataca aattatccac cggaatgtca
     8101 aaaaaacctt gaatctgtcc cgagtgaaga tccaatgtca gcattcgtga ggcgcccgct
     8161 gttgtaatca agtcggccac cagtttagcg ctgattggaa ctcgagggag cactttccga
     8221 tcttggcgcg cataaccgta gtaaggcatc acggcggtga tacgcttcgc cgaagctctc
     8281 ttgaacgcat ccagcatcaa caagagctcc atcaaatgat gatttcccgg cgccgaagta
     8341 gactgcaaaa caaaaacatc agcgccgcgc acgttctcct tgatctcgac cttgctttcg
     8401 ccatcgctga aggtttccac tgtcgctttt cctagcggca ctgccaggta gttgctaatt
     8461 tcccgagcaa gtggaggatt ggagtttccg gcaaagatct ttatgtcggt attctgctgg
     8521 ttcatggtga tggattccca ttccgctttt tacatactcc ccctaccaca tcaatggcaa
     8581 tacagcacca agcacatcgg cgcacgacta agatgaaatt gtctactgcc aaaaacgagt
     8641 tgcgccgttc gacttgaacg gcgcaaccta aactatgata atctgttgat gattacaaga
     8701 ttaagactgg ctgggcggga aggattcgaa ccttcgatac cggatccaaa gtccggggcc
     8761 ttaccgcttg gcgaccgccc aatccccaaa cttctcgaga gaagctcatc cggccctggg
     8821 gcagaggatc aactaaccgt ttcctagagc cctctggctc ccaccctccg cagcaacttt
     8881 ctcatattcg gcgattactt tctcctctaa cattctacgt gtttcgttat tgatgggatg
     8941 cgcaatatct ttataggtcc catcttttcg tttcttgctg ggcatcgaca caaagtatcc
     9001 ggtggttcct ttgataattt ttaaatctct gatcacgaaa caatgatcga gagtgatagt
     9061 tacgtaagcc ctcagcttat cttcgtcgac aggaaagact cttacttccg taacctgcat
     9121 cgagacctcc tcttttgaca ggttaaccga cttcctgact tttgctctga atgggctttg
     9181 cctatatcga tctctttaga ttaacaaacg cactagaaac gtatctaccc catcctcctt
     9241 ctgcaatcgg gacaacgccc gtgcagcgtt tcctctggaa tcaaataggc caaaaaccgc
     9301 agagccacta cccgacatta gagctccggc tgcgccctcc gcaatcaatt ctcttttcaa
     9361 ggattttata cggggatatc gagccacagt tacactttcc aagtcattaa ctaggaacct
     9421 ctgttgaccg taagacttcc taagtggtga aataatgcta gtatttgcca taagttttgt
     9481 caacttagca gcaaatttcc ggtataccca agcagtcgcc acaggaaatc cagggtaaac
     9541 aattactgcc cattcccagg gcagagattt gattggcgta agacgttcgc cgatacctct
     9601 gacgcgcgca ggtctacaat caatgaagaa cggaacatca gcgcctagtc catatgctcg
     9661 tttcttaagc cgccgcgtag agtagttgag cccaaagaga gaattcattg caattagcgt
     9721 cgctgccgca tcggaactgc cgcctcccaa tcccgcccca ataggaattc gttttctgat
     9781 gtgaatttct actctctcc
//
LOCUS       DASUTG010000086         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1199324, whole genome shotgun sequence.
ACCESSION   DASUTG010000086 DASUTG010000000
VERSION     DASUTG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199324"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1067)
                     /locus_tag="VF089_01945"
     CDS             complement(57..1067)
                     /locus_tag="VF089_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172747.1"
                     /translation="MRFKIGSLIFLAAIFGMIAPTFAAQSESIDELYKKALKEEGVLN
                     CYCSLAQINAEKIYPVFEKRFPGIRINHVDATSDKLSARAITEARGGRTLADVVEFGL
                     EDINKIHAQGLILEKAPPEAAEYPANLKGTFWTANNLIFFVGAWNTEKVKKEEEPKYL
                     DDFADPRWKGRLIAEPRDYEVLIGLTHKHKSFEKARAILSKIAANNVEFHKGHSQLAE
                     FIVAGQAAACFTCYSHHYPSRKRKGAPVDYMLTEGAAGIIAVAVMKDAPHPNTAWLFN
                     RWAASEEGQKVYAQGGRTPAHPKVEPTEKIRPQAIYAVGVEDLKQYAKYEKAWKEVFK
                     LR"
     gene            complement(1161..2177)
                     /locus_tag="VF089_01950"
     CDS             complement(1161..2177)
                     /locus_tag="VF089_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6172748.1"
                     /translation="MFLNFRKITWWILLLIVASPSWGNGQTESLDDLYKKALKEGGEL
                     NFYGTLAQINAAKVIPLFEKRFPGLKINHVDATADRLAARAITEARGGKVSADIFQMT
                     LENILQLADQKLVVEKLPAEAAAYPDNLKGSFWIAADLLFITGAWNTNLVKKGEEPKQ
                     FEDFAEPRWKGRLIAEPRDVEILMGLANHKYKNDEKAIALLKKIAANNVEFHKGHSQL
                     AEFLVAGQAAACFTCYAHHYPPRIKKGAPLGYMLTEGVASINATAIAKDSPHPNTAWL
                     FARWSASEEGQKVYAIGGRTPPHPKVEPMEKIRPGKIYPIGTAEIKNWSKYEKIWKDI
                     FKLR"
     gene            complement(2229..3305)
                     /locus_tag="VF089_01955"
     CDS             complement(2229..3305)
                     /locus_tag="VF089_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6172749.1"
                     /translation="MSFAKVFVLLLLALWISSSSALAAQSGAKPSYDEKAVADFYRGK
                     TVRIIVGFSAGGGYDQYSRLIARHMSKYIPGNPAVIVDNMPGAGSIIAANHTFNAAPK
                     DGTVIGNISGPIVLEQLFGSPSIQFDMAKFRYLAVPVSETYLFIVTKKSGVTKFDDLI
                     GGKGKQVSIGAIPGSTVEHAPILARDVLGANLKVVSGYKGTADVRLAIDSGEVDGFFN
                     TWTSSKVTSFDKVKSGEWLVLAQLSEKPIKDLIIPNVPTISQIGKTDEQRLIMKYGTS
                     TPNDFGKVYVLPPGTPADRALALENAFDKVFTDKELMADADKGKLEIDPMLGDEIYKL
                     VKEFLGMSPELKTKLQTALKGGKK"
     gene            complement(3528..4496)
                     /locus_tag="VF089_01960"
     CDS             complement(3528..4496)
                     /locus_tag="VF089_01960"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6172750.1"
                     /translation="MGKGILVTGGSGLVGAYAVGMLLARGERPVVFDVAINERLLAAV
                     GVDTNRVTLVRGDTADLPALISTLRDHQCDRIIHLAAFLGEEVQRRPYSGVRLNFMGT
                     VNVFEAARLEKVSRIIFPSSGTVYLGSIGEGLAKIDESIPVNPPSIYAATKASCEFMG
                     RAYAKRYGFQFICLRYVGGLYGPSPVALKATREVAIQQMIRAAIKGEAAKIKWPYGAA
                     EILYGKDAAKGTVLAVLKEKLKDDLFHIGNGQMVGGEEIVAAIQKNFPGSKIELMKGD
                     NPMPYPESRLASDFSRSKEQLGYQPDYPIDKAVADYAATLKQLEHL"
BASE COUNT          990 a         1274 c         1150 g         1196 t
ORIGIN      
        1 ctcgacatgg cgcctcccga tgatggatga tgatctacag cttattgagg aatagtttac
       61 cgcagcttga atacttcctt ccaagccttt tcatattttg catattgctt cagatcctcg
      121 acacctacgg cgtagatcgc ctggggccgg attttttccg tgggttccac cttcggatga
      181 gccggggttc tgccgccctg cgcatagacc ttctgtccct cttccgacgc cgcccaacgg
      241 ttgaacaacc atgcggtgtt gggatgcggc gcatccttca tcaccgcaac cgcaataatg
      301 ccggcagctc cttcagtgag catatagtcg accggcgcgc ctttcctttt acgggagggg
      361 tagtgatgtg aataacaggt gaaacaagct gccgcctgac cggcaactat aaattccgca
      421 agctgcgaat gtcctttgtg gaattcaacg ttgttcgcgg cgattttcga aagaatcgcg
      481 cgcgccttct cgaagctttt gtgcttgtgc gtcaagccga tcaaaacttc gtaatctctc
      541 ggttcggcaa tcagacgtcc tttccagcgc gggtcggcga agtcatccag gtacttgggc
      601 tcctcctctt tttttacttt ctcagtgttc caggcgccaa cgaagaagat gagattgttg
      661 gcggtccaga atgtcccttt cagattcgcc ggatactccg ccgcttccgg cggcgccttt
      721 tccaggatca atccctgggc gtggatctta ttgatatcct cgagcccgaa ctcaactaca
      781 tcggcgagag ttcggccgcc tcttgcttcg gtgatagcac gggccgaaag cttatcggag
      841 gtggcatcga cgtggttaat tctgattccg gggaagcgtt tctcgaaaac gggataaatc
      901 ttctcggcgt tgatttgcgc cagggagcag taacaattaa gcacgccttc ttccttaagt
      961 gccttcttat agagttcatc gatggactcg ctttgtgccg cgaaagttgg tgcaatcata
     1021 ccgaagatcg ccgctaggaa aatcaagctg ccaatcttaa atcgcatgtc gcctcctctg
     1081 ccgcctttac ggccggcttt agactgctaa attcacgttc ctactcgcca tctgtcgcgc
     1141 tttacgcagc agactattct tcatcttagc ttgaagatat ctttccagat cttttcgtat
     1201 ttgctccaat tctttatctc agccgtcccg atgggataga tttttcccgg ccgaatcttc
     1261 tccatcggct cgactttggg atggggcggc gtgcggccgc cgatggcgta gactttttgc
     1321 ccttcctcgc tggcggacca gcgggcaaac agccatgccg tattgggatg agggctgtct
     1381 ttggcaatcg cggtggcgtt tattgatgcg acgccttccg taagcatata tcccaaaggc
     1441 gccccttttt tgatgcgtgg cggatagtga tgggcatagc aggtaaagca tgcggccgcc
     1501 tgtccggcca ccagaaactc ggctagctgg gagtggccct tgtgaaactc tacgttattg
     1561 gcggcgattt tcttaagcag cgctatagcc ttctcgtcgt tcttgtactt gtggttggcc
     1621 agacccatga ggatctcaac gtcacgcggt tctgcgatga ggcgcccctt ccagcgcggc
     1681 tccgcgaagt cttcgaactg cttgggctct tcgcctttct tgaccaggtt cgtattccag
     1741 gctccggtga taaacagcaa atcggcggca atccagaagc ttcccttcag attgtcggga
     1801 tacgccgccg cttcggcagg gagcttctca actaccaatt tctgatccgc cagttgtaga
     1861 atattttcta gggtcatctg gaagatatca gccgagactt tgccgccacg cgcttccgta
     1921 atcgcgcgcg cggccagtct atcggcagtg gcgtcaacgt gattgatctt caatccggga
     1981 aagcgcttct caaataaggg aatgactttg gcggcattga tttgtgccaa ggtgccataa
     2041 aagttgagct ccccgccttc tttcagcgcc ttcttgtaca ggtcatccag gctctcggtt
     2101 tgtccattcc cccaactcgg agaggcgacg ataaggagga gtatccacca agtgattttc
     2161 ctgaagttaa gaaacattgc gcatccctcc gtagtttatc tctacgctaa ctggactatc
     2221 gccctgtact atttcttacc acctttcagc gcggtttgga gtttggtctt caactcgggg
     2281 ctcatgccaa gaaactcctt aaccagtttg tatatctcgt cgccgagcat tggatcgatt
     2341 tcgagcttgc ctttgtcggc atcggccatg agttccttat ccgtgaatac tttgtcaaag
     2401 gcattttcca acgccagggc acgatcggcc ggggtacctg gcggaagaac ataaaccttg
     2461 ccgaagtcat ttggcgtgct cgtgccatat ttcataatca gccgttgttc atcggttttg
     2521 ccgatttgtg aaatcgtcgg cacgttcggg atgattaggt ccttgatcgg tttttccgat
     2581 aactgcgcca gcacgagcca ctcgccgctc ttcaccttgt cgaatgaagt taccttcgag
     2641 gaagtccagg tgttgaagaa accatccacc tcgccgctgt cgatggccaa tcttacgtcg
     2701 gcggttccct tgtacccgga gacgactttg agatttgcgc ctaaaacgtc acgagccagt
     2761 atcggtgcat gttccacagt cgatcccgga atcgcgccaa tgcttacctg tttgcctttg
     2821 ccaccgatca gatcatcaaa tttagtgacc cccgattttt tagtgacgat aaacaagtag
     2881 gtttcgctca ccggcaccgc aagatagcga aacttggcca tatcgaactg gatggaggga
     2941 ctaccaaaga gttgctccag aacgatcggt cccgaaatgt tgccgatgac ggtaccgtct
     3001 ttaggcgctg cgttgaaagt atgatttgcc gcgataatgc tgccggcgcc gggcatatta
     3061 tcgacgatga cggcgggatt gccgggaatg tatttggaca tgtgtcgggc gatcagcctc
     3121 gagtactgat cgtacccgcc accggcggaa aacccaacga tgatccgcac cgtcttgccc
     3181 cggtaaaagt cggcaacggc cttttcatca tagcttggct ttgcacccga ttgagcagcc
     3241 agagcggaac tcgatgaaat ccacagcgca agcaacagca gtacaaaaac tttggcgaaa
     3301 ctcatagtac tttttgcagc ctccttctct tcaactagca gaacaatcgt gtattcagag
     3361 attagacgac aatggtagat ccgagcttca gcggtggcct tgactgatat caattgttaa
     3421 gccaccgccc tttaagaggg atacttttga agtcataaat ccccttcgct gccgcgcctc
     3481 gtcacaatcc taagttgcta tgagcgcacc tgatgttgct cgcattgcta caggtgctcg
     3541 agctgtttaa gagtcgcggc gtaatcagca accgccttat cgatcggata gtccggttga
     3601 tacccgagct gttcttttga ccgcgagaaa tcgcttgcaa gccgagattc gggataaggc
     3661 attggattat cgcctttcat taactcgatc ttcgagccag gaaaattttt ttgaatggcg
     3721 gcgacaatct cctcgccgcc gaccatctgc ccgttaccaa tgtgaaacag atcatccttg
     3781 agtttttcct tcaatacagc gagcactgtt cctttcgcgg catctttacc gtacaagatt
     3841 tcggcagcgc catagggcca tttgattttc gccgcttcac ctttgatcgc ggcgcgaatc
     3901 atttgctgga tagcgacttc tctggtcgct ttgagcgcca ccggcgacgg accgtagaga
     3961 ccgccgacgt aacgaaggca aatgaactgg aaaccgtagc gtttggcata agcccggccc
     4021 atgaactcgc agctggcttt ggtcgcggca tagatcgaag gcgggtttac cggaatggat
     4081 tcatctattt tcgctagacc ctcgccgatg gatcctaggt aaactgttcc ggaacttggg
     4141 aagataatcc gggagacctt ttccaggcga gccgcttcga agacgttgac cgttcccatg
     4201 aaattcaaac gcacaccgct atagggtcgt cgctgaactt cctcgccgag aaatgccgca
     4261 agatgaataa tgcggtcgca ctgatgatcg cgcagcgttg aaatcaaagc cggcaggtcc
     4321 gccgtgtcgc cgcgaaccag agtgactcgg ttggtgtcaa caccgactgc cgcaagcagc
     4381 cgctcattga tcgctacatc gaaaaccacc ggccgttccc ctcgtgccaa caacatgccc
     4441 acggcatagg ctccgaccaa ccccgaacca ccggtgacaa gaattccctt gcccatctta
     4501 gctcctctcc tctcaggacc tgcctctact atcccaaaca gatctttgag tcaatgtagt
     4561 cgttttcgtc aattacaaca cctttggttg catttttaat gattcgagaa
//
LOCUS       DASUTG010000087         4485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1348313, whole genome shotgun sequence.
ACCESSION   DASUTG010000087 DASUTG010000000
VERSION     DASUTG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348313"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(417..2075)
                     /locus_tag="VF089_01965"
     CDS             complement(417..2075)
                     /locus_tag="VF089_01965"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX6172751.1"
                     /translation="MTPEFTAEEQKRLAAFFTNLDRPIFGLKLPQEVAGALFSRYSRS
                     SKSLRRTFLDEFLGDPELGLNDLLGRQPVNDDSAALKKARAFYDRVLVGYGDDSVAQL
                     GGAHLACENISNVAAKLLEDARIGIAPLEKSTRYVRFDHKDGGGNYLFYREPKIMASR
                     HRDAYLETMNLLFDTYSRQMEPMLASIAKALPIEQVEVRDPTTGKTLPYADANNDDRL
                     RRWAETAYRSTVRAHACDVLRNYLPAATLTNVGMFGAGQAFEYLLSKLYSNQLSEAKA
                     VAGAMHGELNQLMPSFIKRAQSNEYLAQIAASAKKIAAKITKDSPRETEPVVLLDYDA
                     DAEEKVIAAILYGHARHSLQQIRQIVAKIDQPQRRQILQEQFAKRRHRRDKLSRAFEN
                     AYYTFDILGNLGLYRDLHRHRILTQERQEFTTVHGYDTPPEIEEAGFKSEFEECMKRA
                     ADLYEKIFKDLPFEAQYVVPFAYKIRWYMKMNLREALHMCELRTMPQGHPDYRFICQE
                     MWRKIQEVHPALAEAGKFIDWQKYRLGRLQSELRTEFKKSTLLE"
     gene            complement(2240..3439)
                     /locus_tag="VF089_01970"
     CDS             complement(2240..3439)
                     /locus_tag="VF089_01970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6172752.1"
                     /translation="MEKYDVAIIGAGPAGAAAAISLAGKGYRVALIDKERFPRDKLCG
                     DFINPINWPILRELGVENEVLSSEHAKVTAFRITACSGEHAQARLPADNSAPEFGLGL
                     SRTRFDVTLLRKAEHKGAVVLQGCRIKSLEREPRGWELLLDHASGDGEIRAHVLVGAD
                     GRNSWVAHRLGLTSAAETRGRSVGFQIHLDAPHAVRGNIDIHIFPGGYAGLVGVGGNS
                     FNLCLAVDKGRLPREQQAEFLSESLLPQNPYLRELLLNSRQVGAARATYPVYFKPRRS
                     VANGILFIGDAARVNEPVSGEGIYFAMKSALLAAETIHDAFGASDFSATRLRSYERRC
                     RREFKLRRGLNMLLRHLAYRPALLAPLVRFSGKHGRLLDALVHIICSPHSAKIRSHHE
                     ARKGHEG"
     gene            complement(3484..4278)
                     /locus_tag="VF089_01975"
     CDS             complement(3484..4278)
                     /locus_tag="VF089_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6172753.1"
                     /translation="MKRSMAREMMDSPDNPRELLEDDLRNLRLINRYLGNYRAVLGGL
                     ARLIEEQRLQRFSLLDVGTGSADIPALIASWARRHHLTVQLVGLEPDTVTLEAAVNQT
                     RNYPEISLVRGDGRALPLAPDSVDFVLSSQMLHHFPEDDIVNLLRGWSRVARRAIIVC
                     DLVRHPVAYYGIRLVTKAFTRNIMTRTDGPLSVQRSFTLDEWRELFERAGIGPFRLFP
                     KFPFRQITIFSMLEQKSGSRLDEYQSDHKSFAGSSPSPCDLGPGRM"
     gene            complement(4275..>4485)
                     /locus_tag="VF089_01980"
     CDS             complement(4275..>4485)
                     /locus_tag="VF089_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HEX6172754.1"
                     /translation="SGAAGVVAAIMAMKHNFLPPTINCDEPDPECDLNYIPNRGLARP
                     VDLSLCNCIGFGSKNSALVIARGEL"
BASE COUNT         1036 a         1220 c         1243 g          986 t
ORIGIN      
        1 cggatgttga gctcgttagc aatgatttgg ttgattttga actgtgcaac ttcagaaatg
       61 gtattggcca taggaagaat gtgcgaatgc gacggatttt cttggacgtt ctctttggta
      121 agtcgtgatc tcgcaaatag gacagggtta taacaaccag cgggttaaca ccgcgaccat
      181 cactggaata atcaagccaa taaagctatc agaagaatat tttgacttcc agctaaaatg
      241 tgtaagagaa aactataaca agtcatgcgc gaataagaat ggtatgtggg tcgcaccagg
      301 gttagaaagc cgcagtccca cctcagccga gatgctagcc tccccattta ggaatagttc
      361 aaatgtttgc cgcgtgactc caaagcggcc agctctttcg ttagggtatc tggttttcat
      421 tctaacagcg tcgacttctt aaactccgtt cgcaactcag actgcagacg ccctaacctg
      481 tacttctgcc aatcgataaa cttccccgct tcggccaggg ccggatgaac ttcctggatc
      541 ttgcgccaca tttcttggca gataaaacgg tagtcgggat gcccttgggg catggtccgc
      601 agctcgcaca tgtgcagcgc ttcgcgcaga ttcattttca tgtaccagcg aattttgtag
      661 gcaaacggca ccacgtactg cgcttcaaaa ggcaaatcct taaagatctt ctcgtacagg
      721 tccgccgcac gtttcatgca ttcctcgaac tcggatttaa aacccgcttc ctcaatctcg
      781 ggcggcgtat cgtagccgtg cacggtggtg aattcctggc gctcctgagt gaggatgcgg
      841 tggcgatgca aatcgcgata gagcccgaga ttgccgagaa tgtcgaaggt gtaataagca
      901 ttttcgaagg cgcgactgag cttgtcacgc cggtgccggc gcttggcaaa ctgttcctgg
      961 agaatctgcc ggcgctgggg ctgatcgatc ttggcaacaa tctggcgtat ctgttgtagt
     1021 gaatggcgcg catgaccgta aagaatcgcc gcgatgactt tttcttcagc atcggcatcg
     1081 tagtccagca agaccaccgg ctcggtttcc ctcggagaat ctttggtgat cttagccgca
     1141 atttttttcg ctgatgccgc gatctgcgcc aggtactcgt tagattgagc gcgcttgatg
     1201 aatgaaggca tcaattgatt cagctcgccg tgcatcgctc cggcaactgc tttggcttcg
     1261 ctcagctgat tggaataaag cttactcaga agatattcaa aagcctggcc ggcgccgaac
     1321 atcccgacgt tggtcaaggt cgcagccggc agatagttac gtaaaacatc acaagcatgc
     1381 gcgcgcacgg tggagcggta agccgtctcc gcccaacggc gcagccggtc gtcgttgttg
     1441 gcgtcggcat atggcagagt cttcccggtg gtcggatcgc gcacttccac ttgctcgatg
     1501 ggcagcgcct tggcaatcga cgccagcatc ggctccatct gccgcgagta agtgtcgaac
     1561 agcaggttca tcgtttcgag atacgcgtcg cggtggcgcg atgccatgat cttgggctcg
     1621 cggtagaaaa gataatttcc gccgccgtcc ttgtgatcga agcgcacata gcgagttgac
     1681 ttctccagcg gagcgatgcc aatgcgagcg tcttcgagaa gcttggccgc gacgttggag
     1741 atattttcgc aagctagatg agcgccgccc agttgtgcga cggaatcgtc gccatagccg
     1801 accagcacgc gatcataaaa cgcacgggct tttttcagag ccgcgctgtc gtcgtttacc
     1861 ggctggcgac cgagcagatc attcaagccg agctccggat cgccgagaaa ctcatcgagg
     1921 aaggtgcgcc gcaaactctt ggacgagcgg ctgtaacggg aaaacagcgc accggccact
     1981 tcttgcggaa gcttcaaacc aaaaatcggt cggtcgaggt tggtaaaaaa cgctgccaga
     2041 cgtttctgtt cttcggcggt aaattcagga gtcattctgc aaatatactc gcaaggaggg
     2101 aggctgtcat ctgcggaatg tcatcaggtg agtttttctg atatcgccac ggtttaatga
     2161 acgcagaagt cgcaagcaga atagccaaag gcatttcacc acgaaggtca cgaagttcac
     2221 gaaattgaat gatgaactct cagccttcgt ggcctttgcg tgcttcgtgg tgagatcgta
     2281 ttttagcgga atgcggtgag caaatgatgt gaaccagcgc atcgagcaac cgtccgtgtt
     2341 tcccggaaaa acggacgaga ggagccagca gcgcggggcg ataggctaaa tgccgcagga
     2401 gcatgttgag tccccggcga agcttgaact cccggcggca gcgtcgctca taggagcgca
     2461 agcgggttgc tgaaaaatcg ctcgcgccga aggcgtcatg gatagtctct gcggccagca
     2521 gagcgctttt catggcgaaa taaattcctt caccgctcac cggctcattg acccgcgcgg
     2581 catcgccgat aaacaagatg ccgttggcta ccgagcgccg cggtttgaaa tagaccggat
     2641 aagttgcccg ggcggcgccg acttggcggc tgttcaggag caattcgcgc agatatggat
     2701 tttgcggcaa caatgattcc gacaagaact ccgcttgctg ttcccgcggc aacctacctt
     2761 tgtctacggc aaggcaaagg ttaaaagagt tgccgcctac cccaaccaac ccggcgtagc
     2821 cgccgggaaa aatatggata tcgatgttgc ccctgacggc atgtggcgca tcgagatgaa
     2881 tttgaaaacc caccgaccgc cctcgcgttt cggccgcgct cgtcagaccc agccggtgcg
     2941 caacccagga atttctgccg tcagcaccga ctagaacatg cgcgcggatc tcgccatcgc
     3001 ccgacgcatg atccagcaac agctcccatc cccgcggttc acgttctaaa cttttgattc
     3061 tgcagccttg caggacaact gcgcccttgt gctcggcttt ccgcaataaa gtcacatcga
     3121 atctggtacg gcttaagccc agcccgaatt ccggcgcgct attgtccgca ggcaagcggg
     3181 cttgcgcgtg ttcgcctgaa caggcggtga tccggaaggc tgtgaccttg gcgtgctctg
     3241 aagaaagaac ttcgttctcg acgccaagct cgcgcagaat cggccagtta atcggattga
     3301 tgaaatcgcc acacagcttg tcgcgtggaa atctttcttt atcgattagc gccacgcggt
     3361 aacctttgcc cgccagggat attgcagcgg cagccccagc gggcccggcg ccgattatag
     3421 ccacatcgta cttttccatt tcaaatctat aatatttggt tcgtagatgc cgcgagtcag
     3481 aatttacatt ctgcctggac caagatcgca aggagacgga gatgacccgg cgaaactctt
     3541 atgatcgctt tgatattcgt ccagccggct tcctgacttt tgttccagca ttgaaaaaat
     3601 tgtgatctgg cgaaaaggaa atttcggaaa gagacgaaat ggaccgatgc cggctcgctc
     3661 aaacaactcg cgccattcat ccaaggtaaa tgaccgctgc acggagagcg gcccatcggt
     3721 gcgggtcata atgttgcggg tgaaagcttt ggtaaccaga cggatgccat agtacgccac
     3781 cggatggcgc accagatcgc aaacgatgat ggcccggcgc gccacacggg accagccccg
     3841 tagcagatta acgatgtcat cctcgggaaa gtggtgaagc atctgcgatg acagcacgaa
     3901 atcgacagaa tccggagcca acggcagcgc ccggccatcg cctcgtacca aggagatctc
     3961 cggataatta cgagtctgat tgaccgctgc ttctagggtc actgtatccg gctcgagacc
     4021 aaccagctga acggtgagat gatggcgccg tgcccatgag gcgatgagcg ccggaatgtc
     4081 ggcgcttccc gtgccgacat ctaggagaga aaatcgctgt agcctttgct cctcgatcag
     4141 ccgcgccaaa ccgccaagca cggctcggta gttgccgaga tatcggttga tgagtcgcag
     4201 attgcgcaga tcatcttcca ggagctcgcg cgggttgtcc ggcgagtcca tcatttcgcg
     4261 cgccatgctg cgcttcacaa ctctcctcga gcgattacca gggctgagtt cttcgaaccg
     4321 aagccgatac agttgcacag cgaaagatcg acaggccggg caagtccccg attaggaatg
     4381 tagttaaggt cgcactccgg atccggctcg tcgcagttga tggtcggtgg cagaaagttg
     4441 tgtttcatgg ccatgattgc cgcaaccacg ccggctgcgc cggaa
//
LOCUS       DASUTG010000088         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_755060, whole genome shotgun sequence.
ACCESSION   DASUTG010000088 DASUTG010000000
VERSION     DASUTG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755060"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF089_01985"
     CDS             <1..192
                     /locus_tag="VF089_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172755.1"
                     /translation="RMRSTPADPRLTPEQRKKRDFTNSRMVIDATRPYEWKDKFPMVN
                     APSQEVVRMAREKFGYLLK"
     gene            complement(316..810)
                     /locus_tag="VF089_01990"
     CDS             complement(316..810)
                     /locus_tag="VF089_01990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX6172756.1"
                     /translation="MNIKIRDFVGDDAEAVHVVAMKAWRVTYRDIYDSTFIERYVNTN
                     YAPEQLRSLVSRVTESEIFFAVAENETGVIGFCNMGQTQQGIELFRIYLLPEFFGQGI
                     GGLLLAKGEQYLRKQGFSSYFCFVHNANELGKRFYKKQGFHHVSERDENDEWFMEKQL
                     CDRI"
     gene            complement(889..1413)
                     /locus_tag="VF089_01995"
     CDS             complement(889..1413)
                     /locus_tag="VF089_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6172757.1"
                     /translation="MALAIMQIRRLNSPDAKAFQALRLEALRECPSAFSSSYEEEFEI
                     PITVIAGRLAPESGRDMFGAFSGTELAGTIGFGREAGRKLAHKGFIRGMYVSAAFRNQ
                     GIGRQLVSHVLQHAASLSGLRQVTLIVTASNVAAIGLYEKMGFTSFGLEPAALLVDGE
                     FHDEIYMVRFVEQT"
     gene            complement(1610..2626)
                     /locus_tag="VF089_02000"
     CDS             complement(1610..2626)
                     /locus_tag="VF089_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172758.1"
                     /translation="MKSFPIIDADGHVTESLESLRRHLSKEHQSRPLFTSEAWDRSFG
                     GTLGKRNEDPKVQLADMDLDGIDIQVIFPTHLSLNSEKETALAVDIARAYNDWLADFC
                     SADPKRLKGVAMVALQDVSAAVKEVRRAVEELGLVGVMMPTNVRDQDIGRREYWPFYE
                     EVERLGIGLALHGGIRAAERMHGRFDNFIAVHSLSFPFECMAAVTGLIFAGVPETFPK
                     LRIAALEACCGWIPFLMDRLDEEFEKRGAKEAPLLKRKPSEYMSGEQFFYAFELEEST
                     VPYVIDRIGADKLLYSSDYPHWDSSWPKTVRMFQEREDISDANKRQIAWENPQKFYGF
                     EAAS"
     gene            complement(2679..3716)
                     /locus_tag="VF089_02005"
     CDS             complement(2679..3716)
                     /locus_tag="VF089_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6172759.1"
                     /translation="MIHNRHVLWTALLAMWLLLFTPATSAIAQESFYQGKTVRVIVGG
                     SAGGGYDTYTRLIARHLSKHVPGNPNFVVENMTGAGTLISANHVYKVAKPDGLTIGHF
                     IGGLLLQQVLGKPGIEFDGRRFEYLGVPAQDNTVIGISKASGVTSVENWLATKTTLKF
                     GGVGPGSATDDIAKVVRATVGVPMQLVSGYKGTADIRLAINSGELHGVANSWESFKSG
                     WVQEIQSGSVIIVLQMIPDRRHPDLPKVPMITDIVKSEDARKIIQAGIYDYAAIARPY
                     VFPPNTPKDRVVMLRKGLLGTYKDPEFIADAQKARLDMSPLSGEELEKVVARTYKLEK
                     SIIEKLKEILK"
     gene            3877..>4079
                     /locus_tag="VF089_02010"
     CDS             3877..>4079
                     /locus_tag="VF089_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6172760.1"
                     /translation="MKYRYLGKQRLKVSAIGYGCPTFQGKLSAADERRDIDVLHRAID
                     LGINFIDTADHNNGNNEELLAKAL"
BASE COUNT          962 a         1125 c         1040 g          952 t
ORIGIN      
        1 cgcatgcgca gcactcccgc cgacccccgg cttactcccg aacagcgcaa gaagcgggac
       61 tttaccaact cgcgcatggt aatcgatgcg acccgaccgt acgaatggaa ggacaagttt
      121 ccgatggtca acgcgccaag ccaggaagta gtgaggatgg cgcgagagaa gttcggatat
      181 ttgttgaaat agctccgctg ggccgcgcca agttccgagt ttcgcctttc gcattgtaca
      241 gaacagtggg tacagcgctc cttgataagg agcagcttct gacggaggtg tttcggggag
      301 tgttgcgctt aatgttcata tccggtcaca caactgcttt tccataaacc attcatcatt
      361 ttcatcgcgt tcagagacat ggtgaaaacc ctgtttcttg tagaaccgct tgccgagttc
      421 gtttgcgtta tgaacaaagc aaaagtatga gctgaatcct tgcttgcgca agtactgctc
      481 accttttgcc aacaaaagtc ctcctatccc ttgaccgaaa aattctggga gcaggtaaat
      541 tcgaaacaat tctataccct gttgagtctg acccatattg caaaatccga tcacccctgt
      601 ctcattctct gcaacggcga aaaagatctc actttccgtg acgcgactta ccagactgcg
      661 cagctgttct ggtgcataat tcgtgttcac gtaccgttcg atgaaggtcg aatcgtaaat
      721 atcgcgatag gtgactcgcc aagccttcat cgcaacaaca tgaactgcct cagcgtcatc
      781 accgacgaaa tcacgaattt tgatattcat ccgattcgtt gactgctgcg atgcgcgcac
      841 aactactaag taactccgtc gctggagacg gtccgctgca acgaacagtc aagtttgttc
      901 aacaaagcgc accatgtaga tttcatcgtg aaactcaccg tcgacgagaa gcgctgccgg
      961 ctcgaggccg aaggacgtga agcccatctt ttcatatagt ccaattgcgg ctacgtttga
     1021 cgcggtcact atcagagtga cttgacgcag accactcagt gaagcggcat gttgcaaaac
     1081 gtgcgagacg agttgccggc caatgccctg attcctgaaa gcagcgctga catacatgcc
     1141 tcggatgaat cccttgtgcg cgagcttgcg cccagcttcg cgtccaaaac cgatagtgcc
     1201 tgcaagttct gtcccgctga acgcgccaaa catatctcgg ccagattcag gagccaagcg
     1261 gccagcgatg accgtgatgg gtatttcgaa ttcttcttcg tagctggagc tgaatgcgct
     1321 agggcactcg cgcagcgctt ctaggcgtag tgcctgaaac gcttttgcat ctggggaatt
     1381 cagtcggcgg atctgcatga tagctaacgc catgaagagg cgtaatctgc cgcgttcgtc
     1441 tcaacccaat gataacaccc gacatcgctc catctgacca tcagtgctga tgagtccgga
     1501 gtcccgaaaa tacagaagct cgtaattcga cggcaactcg taaatcacat cgatacgaat
     1561 ccaagcctcg ttgaacaaat gccggtccgc acatcaaaca accggtatgt tatgacgctg
     1621 cctcaaagcc gtagaacttc tgcgggttct cccaggcaat ctgacgtttg ttggcatccg
     1681 aaatatcttc acgttcttga aacattcgca ctgtcttggg ccaagagcta tcccagtgtg
     1741 gataatcgga agaatacagc aatttgtcgg cgccgatgcg atcgatgaca taagggacgg
     1801 tcgattcttc cagttcaaaa gcgtagaaga actgctcgcc gctcatgtat tcgctgggtt
     1861 ttcttttcag gagcggcgct tctttggcgc cgcgtttttc gaattcttca tccagccggt
     1921 ccataagaaa gggaatccag ccgcaacaag cttcgagcgc ggcgatgcgc aacttgggga
     1981 atgtctcagg cacgccggca aagatcaggc cggtcacagc cgccatgcat tcaaacggga
     2041 acgacaggct atgaacggcg ataaagttat caaaccggcc atgcatccgc tcggcggcac
     2101 ggatgccacc atgcaatgcc aggccgatgc cgaggcgctc gacttcttca tagaacggcc
     2161 agtactcgcg ccggccgatg tcctggtcgc gcacgttggt gggcatcatc acgcccacta
     2221 atccgagttc ttcaacggca cggcgaactt ccttaaccgc agcgcttacg tcctgtagag
     2281 caaccatcgc cacgcctttg agacgtttcg gatcagcgga gcaaaaatcc gccagccaat
     2341 cattgtaggc tcgcgcaatg tccacggcca gagcagtctc cttctctgag ttaagagaaa
     2401 ggtgagtcgg gaaaattact tgaatatcga tgccgtcgag atccatatcg gccagctgca
     2461 ctttgggatc ctcgttgcgt ttgccgagcg tcccgccgaa ggatcggtcc caagcttccg
     2521 aggtgaagag cggccggctc tgatgctcct tacttaagtg ccttctgagg ctttccaatg
     2581 attcggtcac gtgtccatcg gcatctatga ttggaaaact tttcatatag attctcctga
     2641 tcgtgtttac gcgctcgctt cgaattccag cacgtaagtt actttaagat ttcttttaac
     2701 ttctcgatta tcgacttttc aagcttataa gtgcgagcga ccactttctc caactcttcg
     2761 ccgctaagcg ggctcatgtc cagccgtgcc ttttgcgcat ccgcgataaa ctccggatct
     2821 ttataggtac caaggagacc cttgcgaagc atgaccacgc ggtccttggg cgtatttggc
     2881 ggaaacacgt atggacgagc aatcgcagcg tagtcgtaaa ttcctgcttg gataattttt
     2941 cgcgcatctt cgctcttaac gatatcggta atcatcggca ccttgggtaa atccggatgg
     3001 cgccggtccg gtatcatctg caagacgatt atgacggagc cggactgaat ctcctggacc
     3061 cagccggatt tgaatgattc ccaggaattg gcgacaccgt gcaactcgcc gctgttgatc
     3121 gccaatcgga tatccgcggt acctttataa ccactgacca actgcatcgg cacgcccacc
     3181 gtggcgcgga cgaccttggc gatgtcgtcg gtggccgacc ccggcccgac tccgccgaac
     3241 ttaagggtgg tcttggtcgc caaccagttt tcgacgctgg tcacgccgct cgctttgctg
     3301 atgccgataa ccgtgttgtc ttgcgccggt acacccaggt attcgaatct tcgtccgtcg
     3361 aattcgatgc ccggcttgcc tagcacctgc tggagaagca aaccgccgat aaaatggccg
     3421 atggtcagac cgtccggttt ggcaactttg tagacgtgat ttgccgaaat caaagttccg
     3481 gcgccggtca tgttttcaac gacaaagttc ggattgcccg gaacgtgttt gctgagatgg
     3541 cgtgcgatca ggcgggtgta cgtgtcgtag ccgccgccgg cggaaccgcc gacaatgact
     3601 ctgaccgtct tgccttggta aaatgactcc tgcgcgatgg ctgacgtcgc cggtgtaaag
     3661 agcagcaacc acattgccaa caatgcggtc cagagaacgt gcctgttgtg gatcatgaga
     3721 tctcctttgt ggttttcgac ggacgcaatc ggccttcagt gaaagtccgg atgcgtccgc
     3781 agagtctcgg attattagca gaaataatcg tgcctgcaac ttgcaatgca gcgccggcaa
     3841 ggacaatagt tgctccaagg aaaggagcat cgttgcatga agtatcggta cctgggcaag
     3901 caacgactta aagtatccgc catcggttat ggctgtccaa catttcaagg aaagctcagc
     3961 gccgcggatg aaaggcgaga catcgatgtg ctccatcggg ccatcgatct gggcatcaac
     4021 tttatcgaca cagccgatca caacaacggt aacaatgaag aactcttagc caaagccct
//
LOCUS       DASUTG010000089         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1051685, whole genome shotgun sequence.
ACCESSION   DASUTG010000089 DASUTG010000000
VERSION     DASUTG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051685"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4007)
                     /gene="rpoC"
                     /locus_tag="VF089_02015"
     CDS             complement(<1..4007)
                     /gene="rpoC"
                     /locus_tag="VF089_02015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6172761.1"
                     /translation="MEDLFNLFERPKNPLSFNAIRVSVASPEKVRSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMRHRGVVCEKCGVEVIQSKVRRERM
                     GHIELATPVVHIWFLKSLPSRIGALLDMSLKDIEKVLYFESYIVIDPGETPLQYKELL
                     SEARYRKAMEEFGGRFTAEMGAEAVRKILKAVDVVKLSEDLRQEMRDANSEVRRKKLA
                     KRLKVLNAFRTSGNRPEWMVMEVVPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSDMLKGK
                     TGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIYNKLEERGFVT
                     TIKSAKKMVEKERPEVWDILDEVIREHPVMLNRAPTLHRLGIQAFEPILIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLSVEAQVEARALMMSTNNILSPANGKPIIVPTQDIVL
                     GLYYMTRDRLQAKGAGRIFSSPTEVRIAYDQGEIEIQAPITVRMNGERVETTVGRILV
                     YEVVPSEISFKEVNRVLKKKELANLIDISYRLAGNKATVILADRLKDIGYRHATKAGI
                     SIAIKDMVIPPNKEVLLKSAHEDVREIEEQYKNGLITDGERYNKVVDIWAEVTDRIAD
                     EMLRDLGSEKVTDETGRETSIPSFNPIFMMADSGARGSAQQIRQLAGMRGLMAKPSGE
                     IIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDSVISD
                     PDCGTMDGIEITPLMEGGEIIEPLGDRVLGRVALEDVRDPFSGDIIVKANQEINEDLV
                     QRIEDAGLERIKIRSVLTCQSKRGVCVMCYGRDLGRGHMVNHGEAIGVIAAQSIGEPG
                     TQLTMRTFHIGGTASRRAEQTTLESRNDGYIKFINVSTVVNREKDLVVMNRNGELAVV
                     DYPEGEKGRERERERYPVVYGAKLKKKDGEKIKAGELIAEWDPYTIPILTEMSGVAKF
                     GDVVEAITMEEKVDERTGLSTKVIIDSKDVDKRPRISIKDDGGQTARMSATSEARYLL
                     PVGAHINVQEGQTVYAGDAIAKIPRETTKTKDITGGLPRVAELFEARKPKEFAVISEI
                     DGIVSFGKDTKGKRKVIVTPEVGEPREYLIPKGKHISVHETDRIRAGEPLMDGSSNPH
                     DILNILGEKALAKYLVDEVQEIYRLQGVRINDKHIEVIVRQMLRRVRIKEVGDTEFLV
                     GDQVEKWRFDEENQRAIAGGGKPAVAEPLLLGITKASLSTESFISAASFQETTKVLTE
                     AAIHGKIDLLCGLKE"
     gene            complement(4055..>5863)
                     /gene="rpoB"
                     /locus_tag="VF089_02020"
     CDS             complement(4055..>5863)
                     /gene="rpoB"
                     /locus_tag="VF089_02020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6172762.1"
                     /translation="SNQNTCVNQRPIVRIGDQVKAGDVIADGPSTEMGELALGRNVLV
                     ALMPWGGYNFEDSILISERVVKEDIYTSIHIEEFECVSRDTKLGPEEITRDIPNVGDE
                     ALTDLDDSGIIRIGAEVKPSDILVGKITPKGETQLSPEEKLLRAIFGEKAGEVRDTSL
                     RVPPGVEGTIINVRIFSRKGLGKDERSKAIEDEEIIRLRKDQHDEVRILQDGALRRLK
                     KLLVGRKLANRLSDDSRKLLLVKGKEITDEDLDQLPMSYWSEIRVDNESVEAELDRIV
                     ESTAGQVKLIKEVFEDKIEKLRGGDELPPGVIKMVKVFVAIKRKLQVGDKMAGRHGNK
                     GVISRILPEEDMPYLEDGTPVDVVLNPLGVPSRMNVGQILEAHLGWAARSLGHQIDQL
                     MREHRQEADGLRKRLKEYLNTKEASELIDGAKDADVLRLAEKYRDGVRLASPVFDGAA
                     EDEIFGLLRKADLPASGQVTLYDGRSGEPFDGKVTVGVMYIMKLHHLVDDKIHARSTG
                     PYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEMLTVKSDDVVGRTRMYEA
                     IVKGDNLLEPGLPESFNVMVKELQSLGLDVDLVEEK"
BASE COUNT         1258 a         1727 c         1386 g         1492 t
ORIGIN      
        1 ttctctttaa gaccgcagag taaatcaatc ttgccgtgaa tcgcagcttc ggtcagcact
       61 tttgttgttt cttgaaacga agctgccgag ataaagcttt cggtcgacaa cgaggccttg
      121 gtgattccaa gaagcagcgg ctcagccaca gccggcttgc cgcctcctgc gatcgcacgt
      181 tgattctcct catcaaagcg ccatttctcc acttggtcgc cgacgagaaa ttcggtatcc
      241 cctacctctt tgatgcgcac gcggcgtaac atctgtctaa cgatgacttc gatatgctta
      301 tcgtttatgc gcacgccttg aagccgatag atctcctgaa cttcgtcaac tagatacttg
      361 gccagcgcct tctcacccaa gatattcaga atatcatgcg ggtttgaaga accatccata
      421 agaggctccc cggcgcgtat ccggtctgtc tcatgcacgc taatgtgctt gcctttcggg
      481 atcagatact cgcgcggttc cccaacttcg ggggtgacga tcaccttgcg ctttcctttg
      541 gtgtctttac caaaggaaac gattccatcg atttcactga tgactgcgaa ctctttcggc
      601 ttgcgcgctt cgaacaattc agccactcgg ggcaagccgc ccgtgatatc ctttgtcttt
      661 gtcgtttcgc gcgggatttt cgcaatcgca tcacctgcgt aaaccgtctg accctcttgc
      721 acattgatat gcgcgcccac gggtagcaaa taacgagctt ccgacgttgc tgacatacgc
      781 gccgtctgtc caccatcgtc tttgatagaa attctcggcc gcttatcgac gtcctttgag
      841 tcgataatca ccttcgtgga cagcccggtg cgctcatcca ccttctcttc catcgtgatc
      901 gcttcgacga catcgccaaa cttcgccacg ccgctcattt ctgtcaaaat cggaatcgtg
      961 tacggatccc attccgcaat caactcccca gctttgattt tttctccatc cttctttttc
     1021 agtttggcgc cataaaccac ggggtaacgc tctcgctccc gctccctacc tttctcacct
     1081 tccggataat cgacgacggc gagctcgcca ttgcggttca tcaccaccag atccttttcc
     1141 ctattgacga cggtgctgac gttgatgaac ttgatgtaac catcattgcg cgattcgaga
     1201 gtagtctgct ctgctctccg gctcgcagta ccaccaatgt gaaacgtacg catggtgagc
     1261 tgtgttcccg gctcaccaat cgattgcgcc gcaataacgc cgatcgcttc gccatgattg
     1321 accatgtgtc cgcggccgag atcacggccg taacacatca cacacacgcc tcgcttcgat
     1381 tggcaggtca atacggaacg aatctttatc cgctccaaac cggcatcttc aatacgctgc
     1441 accaaatctt cgttaatctc ctggtttgct ttaacgataa tgtctccaga gaaaggatcg
     1501 cgcacatcct cgagcgcaac tcgcccgagc acgcggtcgc cgagcggctc gataatctca
     1561 ccgccttcca tcagcggcgt gatctctatc ccgtccatcg tgccacaatc agggtcggaa
     1621 ataaccgagt cctgagctac gtctacaaga cgtcgagtga gataacccga attggcggtc
     1681 ttcaatgcgg tatcagccaa ccccttgcgt gcgccgtggg ttgagatgaa gtactgcaaa
     1741 accgtcaatc cctcgcggaa attcgcggta atcggcgtct caataatttc tccggaaggc
     1801 ttggccatta acccgcgcat gccggctaac tgacggatct gctgcgcgct gccgcgagca
     1861 ccggaatcag ccatcatgaa gatcggattg aagctcggaa tcgaagtttc gcgtccggtt
     1921 tcatcagtaa ctttctcgga accaagatcg cgtagcattt catcggcgat tcgatcagta
     1981 acctcagccc agatgtcaac cactttgttg tagcgctccc cgtcggtgat aagcccgttc
     2041 ttgtattgct cctcgatttc acgaacgtct tcgtgcgcgc tcttgagcaa aacctcctta
     2101 ttgggcggaa tcaccatgtc ctttatggcg attgagatgc ccgcttttgt ggcgtgccga
     2161 tatccgatat cctttaaccg gtcggcgaga ataacagtcg ccttgttgcc cgccagccgg
     2221 taactgatat cgatcaagtt tgccagctct ttcttcttta gaactcgatt gacttccttg
     2281 aatgagatct ccgacggcac aacctcatat actagaattc ggccgacggt ggtctcgact
     2341 cgctcaccgt tcatgcggac ggtgatcggc gcttgaatct caatctcacc ctgatcatag
     2401 gcgatacgaa cttccgttgg gctgctaaag atgcgcccgg ctcctttggc ttggagccga
     2461 tcgcgggtca tgtagtagag ccccagcacg atatcctggg tcggcacgat gataggctta
     2521 ccattggcgg gagacagaat gttgttggtg gacatcatca gggcgcgagc ctccacctga
     2581 gcttcgaccg acaagggaac gtgcaccgcc atctgatcac cgtcgaaatc agcgttatac
     2641 gccgcgcata ccaagggatg aagctgtatc gcctttccct cgatcaggat cggctcaaaa
     2701 gcttgaattc caagccggtg tagcgttgga gcgcggttta gcatcaccgg gtgctcgcga
     2761 atgacctcgt cgagaatatc ccaaacctcg ggacgttctt tctcgaccat tttttttgcg
     2821 ctcttgatgg tcgtaacgaa gccgcgctct tcgagcttgt tatagataaa cggcttgaac
     2881 aactccaaag ccattttctt cggcagaccg cattggtgta gccgcagttc cggtccgaca
     2941 acgatcaccg aacgtccgga ataatccacc cgtttgccaa gtaggttctg gcggaatcta
     3001 ccggttttac ctttaagcat atcgctcaac gacttaagcg gtcgacggtt ttgtcccgta
     3061 atagctcgac cacgccggcc gttatcaaac agagcgtcaa cggcctcttg gagcatgcgt
     3121 ttctcattcc gaatgatcat atccggtgcg cttaactcca tcaggcgctt gagccggtta
     3181 ttacggttaa tgacccgccg gtagagatcg tttaaatccg aggttgcgaa gcggccgcca
     3241 tcaagtggta ccagaggccg tagatccggt ggaataaccg ggacaacttc catgaccatc
     3301 cactcgggtc gattccccga ggtcctaaaa gcgttgagga cttttaatcg ctttgccaac
     3361 tttttgcgcc gaacctcgga gttcgcatcg cgcatctctt ggcgcagatc ttctgaaagc
     3421 tttactacgt cgacggcttt gaggatcttg cgaaccgcct ctgcgcccat ctccgcggtg
     3481 aatctccccc caaactcctc catcgctttg cgataacgcg cctcgctcag cagctccttg
     3541 tattgcaaag gagtctctcc cgggtcgata acgatatagg actcaaaata gagaaccttc
     3601 tcgatgtcct tcagggacat gtcgagtaag gcgccaatcc gactcggcag gctcttaaga
     3661 aaccagatat ggacgaccgg cgtggccagc tcaatatggc ccatccgctc acggcgcacc
     3721 tttgactgaa tcacctcgac gccgcatttt tcacagacaa cgcctcggtg gcgcatgcgt
     3781 ttatacttgc cgcagttaca ttcgtaatcc ttggtcggac cgaaaatctt ggcgcaaaat
     3841 agaccatctc gttctggctt aaacgttctg tagtttatcg tctccggctt cttcacctcc
     3901 ccgtgcgacc aagaccggac tttttccggg gaagccacag agactcggat tgcattaaaa
     3961 cttagcggat ttttcggtcg ttcaaatagg ttaaaaagat cttccataaa gatccacctc
     4021 cagcatcagc gcagtggggc tccacagggc taagctattt ttcctcgact agatcaacat
     4081 cgagccccaa gctctggagc tccttaacca tcacgttaaa cgattcgggc aaccctggtt
     4141 cgaggagatt gtccccttta acaatcgcct cgtacattcg cgttcgcccg acaacatcat
     4201 cagactttac ggtcagcatt tcttgaagag tataagcagc gccgtaagcc tccaacgccc
     4261 aaacctccat ttctccgagc cgctggccgc caaactgagc cttcccaccc aggggttgct
     4321 gtgtaacgag agaataggga ccggtggagc gagcatgtat tttgtcatca accaagtgat
     4381 gcagtttcat gatatacatg acgccaactg ttactttacc gtcgaagggc tcgccgctgc
     4441 ggccgtcata tagagtcact tgaccgctag ccgggagatc ggctttgcgc agcaaaccaa
     4501 aaatttcatc ttccgccgcg ccgtcaaaaa ccggcgacgc caagcgcacc ccgtcgcgat
     4561 acttctcggc cagccgtaat acgtcagcgt ctttggcgcc gtcgataagc tcgctagctt
     4621 ccttggtatt aagatactcc ttaaggcgct tgcgtagacc gtctgcttcc tggcggtgtt
     4681 cccgcattag ttgatcaatt tgatgcccga gactgcgcgc tgcccagcca agatgagctt
     4741 cgagaatctg gcccacgttc atacgtgaag gcacgcctag cggattgagc accacgtcga
     4801 ccggagtgcc gtcttccaga tacggcatgt cctcctcggg taggatcctg gaaataacgc
     4861 ctttgttacc atggcggccc gccatcttgt cgccaacttg cagcttccgt tttatggcga
     4921 cgaagacttt caccatcttg atgacacccg gcggcagctc atcgccgcct ctgagttttt
     4981 caatcttgtc ttcgaatact tctttgatca gcttgacttg tcctgccgtg ctttcaacaa
     5041 tgcggtcgag ctccgcttcc actgattcgt tatcgacgcg gatctcgctc caatagctca
     5101 tcggcaactg atccagatct tcgtcggtga tctccttacc tttaacgagc agcagttttc
     5161 ggctgtcgtc gcttagtcgg ttcgccagtt tccggccgac taatagtttt ttaagacgcc
     5221 taagcgcgcc atcttgaagg attcggactt catcatgctg atctttacgt agccgtataa
     5281 tctcttcatc ctcgatcgcc ttgctcctct catctttgcc caaccctttg cgcgagaaga
     5341 tccggacatt gatgatcgtg ccttcaactc cgggtggaac gcgcagcgat gtatcacgga
     5401 cctcaccagc cttttcgccg aatatggcgc gcaagagttt ttcttcaggc gacagctgag
     5461 tctcgccttt gggagtaatt ttccccacga ggatatcact aggcttgacc tcggcgccga
     5521 ttctgatgat accgctgtca tcaagatctg tcagggcttc atcgccgacg ttcgggatat
     5581 cgcgagtgat ttcttccggt ccgagtttgg tgtcccgcga aacacattca aattcttcaa
     5641 tatgaatcga agtgtagatg tcttctttaa cgacacgctc gctgatcaag atggaatctt
     5701 caaagttgta gccgccccag ggcatgagcg cgacaagaac attgcggccc agcgcaagct
     5761 ctcccatttc tgtggagggc ccgtcggcga taacgtcgcc ggcttttacc tggtcaccaa
     5821 tcctgacgat cggtcgttga ttgacgcaag tattctgatt cga
//
LOCUS       DASUTG010000090         7181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1793439, whole genome shotgun sequence.
ACCESSION   DASUTG010000090 DASUTG010000000
VERSION     DASUTG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF089_02025"
     CDS             <1..504
                     /locus_tag="VF089_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172763.1"
                     /translation="AYGSEFEKVVVAVYGRNQQGSPVQLNREQMRFTYRDSHLPPGTV
                     ITRVRLSLHRQESSKVSMKVRELVTKRKSSQPSGYPNSGSMFRNPAGDYAGRLVEAAG
                     LKGKQIGRAQISPKHGNFIVNLGGASAEDVRQLMELARAEVNAQFGVLLMPEVKLIGR
                     WPSAKAE"
     gene            510..1325
                     /locus_tag="VF089_02030"
     CDS             510..1325
                     /locus_tag="VF089_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX6172764.1"
                     /translation="MQLAVSRKDNRKKDKFPWRRRLATLGILVIVAGGVWMLYGWREQ
                     VASASVAARRFIFENPYFAVREIQVRGGDRVRGNEIISMAGLRHGMNLWNIEPEKIEK
                     KVSKHPWVRRALVRREFPRRVVIEIEERRPKAIVAAGKLYYVDADGILFKEVAQGENV
                     RFPMLTGIRTEELGSSDPAVRRRIQDALRLGDLMAKDSHALSEIHFDAPDRLVVYTTA
                     HPVAIHMGWGDWEGKLQRLDRVLTLWRGKEEKLGSLDVSFQDQVVTRLRRLPQ"
     gene            1350..2579
                     /gene="ftsA"
                     /locus_tag="VF089_02035"
     CDS             1350..2579
                     /gene="ftsA"
                     /locus_tag="VF089_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX6172765.1"
                     /translation="MAKKHSIIVGLDIGTSKICSAVGEMTEHGVEIIGIGSHGSLGMR
                     KGVVINIDNTVASIKKAVEEAALMAGCEINTVFTGIAGSHIKAFNSHGVVAVKTREVT
                     QRDLDRVLDAAKAVAIPTDQEVVHVLPQDYIIDDQDGIKDPLGMSGVRLEARVHIVTG
                     ATTAMQNVVKCCNRSGLHVADVALMPLACADAVLTEDEKDLGVALADMGSGTTDITIF
                     HDGTVKHTAVLPIGGNHVTNDIAAGLRTPFADAERIKLRYGCAKASMVAEQVRLEVPS
                     SAGKPQRSVSRQILCEIIEPRMDEVFQLIRKEIVKSGYEDYLGAGVVMTGGSMLLPGA
                     VEAAEIATGVPVRLGTPRHVGGLVDVIANPIYSSAIGLVLYGVKRQERSFFRVRENAT
                     ILSKVKHRMSDWLSDFF"
     gene            2621..3886
                     /gene="ftsZ"
                     /locus_tag="VF089_02040"
     CDS             2621..3886
                     /gene="ftsZ"
                     /locus_tag="VF089_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX6172766.1"
                     /translation="MFEFVEEFNLNARIKVIGIGGGGGNAVNTMIGGKLNGVEFLVAN
                     TDAQSLDASRAPVRIQLGGAVTKGLGAGANPEIGRRAALEDQETIKEYLAGSDMIFIT
                     AGMGGGTGTGGAPVIARVAREVGALTVGVVTKPFIFEGKKRMRQAEEGIAELKSSVDT
                     LIVIPNQRLLSIAAKTTTMLEAFHKADEVLLQAVRGISDLIITPGLINLDFADVRTVM
                     AEMGLALMGASTATGENRAIEAAQRAISSPLLEDISIQGARGVLINITGGPDLCLHEV
                     NEAASMIQEEAHDEANIIFGAVIDETMLDEIRITVIATGFGEGKEDRKPVITSSGHVS
                     TMNTFNSMNHANNSVPNVANVNPIKNKKVVHLGTIVDDLDAPTWQRKKPGSEEVETVT
                     LSKSTLQLSTGQEDDDKYDVPTFLRRQMD"
     gene            complement(4171..5256)
                     /gene="ddlA"
                     /locus_tag="VF089_02045"
     CDS             complement(4171..5256)
                     /gene="ddlA"
                     /locus_tag="VF089_02045"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX6172767.1"
                     /translation="MKNKLRVAILFGGRSAEHEISLISARNIVAAMDKSKYEVIAIGI
                     DRQGRWSYDNGAGLLRDEQKQVMPFNDAQLTAILPGAKQSSLVRSGGASLGVDVVFPV
                     LHGPFGEDGTVQGLLKLANLPFVGASVLGSAIGMDKDVMKRLLRDARVPIGRFQVYGR
                     STADRINFARLKKELGQPLFVKPANLGSSVGISKVESRSELEAAVKTAFRFDNKILIE
                     EFIEGREIECSVLGNDNPIASLPGEIVTGYDFYSYDAKYIDDRGSRLEIPAKLPKAIV
                     RSVQDIAITSYKTLCCEGMARVDFFVKANGQVLVNEINTIPGFTQISMYPKMWEASGI
                     PYPKLIDRLIQLALQRFRQEQNLRTSV"
     gene            complement(5325..6503)
                     /locus_tag="VF089_02050"
     CDS             complement(5325..6503)
                     /locus_tag="VF089_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6172768.1"
                     /translation="MIPRRALLLLFLLLLGIPVSATAQKGTIKIAVQAPLSGEQAALG
                     EHIKLGAQLAVEEGSKSIKVMGYDLVFVPYDDQAKPEVGVANARNLVADPDVLVLVGH
                     FNSGVALPASEVYKDAMLAMISPANTATEITDRGYANVNRVCGRDDVQGPVGARFAAQ
                     DLKLKSVYIIHDKTLYGQGVADNFRNEAKKLGLNVLGYDGTEERANFAPMIIPLKARN
                     PDLVYFGGIYHQGGLLLKQMREKGVKAVFMGPDGLDSSEMVKIAGQHVTGSYYTTVAG
                     PPDAYPETAAFAKKFKQRFGKEVESFAMYGYDAAQVGIKAVEQTLQANGGKKPSREEV
                     SAAVRKLKNFRGVTGQISFDNKGDPVKAKYFVLQFEKRNYPGKVVKVIEQQAPQAKKS
                     "
     gene            6630..7025
                     /gene="acpS"
                     /locus_tag="VF089_02055"
     CDS             6630..7025
                     /gene="acpS"
                     /locus_tag="VF089_02055"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6172769.1"
                     /translation="MIISVGIDLVEVERIRAALEHPRIGLRFRERVYTQREIAYCERR
                     QRGRFQSYAGRFAAKEAVMKALGRGWGAQVRWLDIEVARAISGKPDIVLHSKTAQLAE
                     KLGIQQWAVSITHTDQHGMAYVIAQDERR"
BASE COUNT         1698 a         1817 c         2056 g         1610 t
ORIGIN      
        1 gcctacggat cggaattcga gaaagtggtc gtcgctgttt acggcagaaa tcagcaagga
       61 agtccggttc aattaaatcg agaacagatg agattcacct atcgtgattc gcatttgcca
      121 ccggggaccg ttatcacgcg cgtgcgcttg tctctgcatc ggcaagagtc gtccaaagtc
      181 agcatgaaag tccgtgagct ggtgacgaag cgaaagagca gccaaccttc gggctacccg
      241 aattccggct caatgtttcg caacccagcg ggagattacg ccgggagatt agtcgaggct
      301 gccgggctca agggaaaaca aattggccgg gcgcaaattt ccccaaagca cggcaatttc
      361 attgtcaatc taggcggcgc cagcgctgaa gatgtgcgtc aactgatgga gctggcgcgc
      421 gctgaagtca atgcccagtt cggtgttctc ttaatgcccg aagtaaagtt gatcggcaga
      481 tggccgtcgg cgaaagcgga gtaagcgcga tgcaattggc agtttctcga aaagacaatc
      541 gcaaaaaaga taaattccca tggcgccgcc ggctcgctac cctcgggatt ctggtgatcg
      601 tcgcgggtgg agtttggatg ctctatgggt ggcgcgaaca ggtcgcttcc gcgtctgttg
      661 cggcgcggcg gtttattttc gaaaacccct actttgccgt gcgcgaaatc caagttcgcg
      721 gcggcgacag agtgcgcggg aatgagatta tttcgatggc gggattgcgg catggcatga
      781 atctttggaa catcgagccg gaaaaaatag agaagaaggt ttcaaagcat ccctgggtgc
      841 ggcgtgcgct ggttcggcgc gaattccctc gccgtgttgt catcgaaata gaagagcgtc
      901 gacccaaagc gatcgtcgct gccggcaaac tttactatgt cgatgccgat ggaattcttt
      961 ttaaagaagt agcgcaagga gaaaatgtca gattcccgat gctaaccggc attcggaccg
     1021 aagagctcgg atcgtcggat cctgcggtac ggcgaagaat acaggatgcc ttacgactgg
     1081 gcgatctgat ggctaaggat tcgcacgcgc tgtccgagat ccactttgat gcgccggatc
     1141 gattggtagt ctatacgacc gctcatccag tagccattca catggggtgg ggtgattggg
     1201 aagggaaact tcaacgtttg gatcgcgtat tgactctgtg gcggggaaaa gaggaaaaac
     1261 tcggctcctt ggatgtaagc tttcaggatc aagtcgtaac gcggttgcgg cgtctgccgc
     1321 agtaaggaaa agaattcggc aagacagata tggcgaagaa acacagcatc atcgtgggat
     1381 tggacatcgg tacgtctaaa atctgcagcg ccgtcggtga gatgaccgag catggcgtgg
     1441 aaattattgg catcggttct cacggctcct tgggaatgcg caagggggtt gtcatcaata
     1501 tcgacaacac cgtggcatcc atcaagaagg cagttgaaga agcggcgcta atggccgggt
     1561 gtgaaatcaa tactgttttt actgggatcg ctggaagtca catcaaagcc ttcaatagcc
     1621 acggcgttgt cgcggttaag accagagagg taactcaaag agacctggac cgggtgctgg
     1681 acgcggccaa agcggtggcg attccaaccg atcaggaggt cgttcatgtt ctgcctcagg
     1741 attacatcat cgacgatcaa gacggcatca aagaccccct ggggatgtcc ggggtaagac
     1801 tcgaagccag agttcacatt gtgaccggag caaccacagc catgcaaaat gtcgtcaagt
     1861 gctgtaatcg ttcggggctt cacgttgccg atgttgcact gatgccgttg gcatgcgcgg
     1921 atgcggtact cacggaggac gagaaggatc tcggtgttgc cttggccgat atgggaagcg
     1981 gcacgaccga tatcacgatt tttcacgatg gcacggtcaa acacacagcc gttctgccga
     2041 ttggcgggaa tcatgtcacc aatgatatcg ccgctggtct tagaacacct ttcgcggatg
     2101 cggagcggat caagctgcgt tacggctgtg ccaaggcatc gatggtcgct gaacaggtgc
     2161 gattagaagt tcctagctct gcgggtaagc cgcagcgctc tgtatcgcgg caaatcttgt
     2221 gtgagatcat tgaaccgcga atggacgagg tctttcagtt gatccgtaag gagatcgtca
     2281 aatcaggcta cgaagattat ctgggcgcag gtgtggtgat gacgggcgga tcgatgttgt
     2341 taccgggcgc tgtcgaagcg gccgaaattg ccactggggt tcccgtacgg ctgggcacgc
     2401 caaggcacgt cggcggtttg gtcgacgtta tcgcaaaccc aatctattcc agcgctatcg
     2461 gattggttct ttatggcgtc aagcgccagg agcgcagttt tttccgtgtg cgagagaatg
     2521 cgaccatcct atccaaggta aagcatcgaa tgtcggattg gcttagtgat tttttctgaa
     2581 acagtaacag atcttaaccg tagtagaagg aggaaagctt atgtttgaat ttgtcgagga
     2641 gtttaatctg aatgcccgta tcaaggtcat cggcatcggt ggcggcggtg gtaatgcggt
     2701 gaacacgatg atcggcggta agctgaacgg tgtagaattc ttggtggcaa acactgacgc
     2761 gcagtccctc gatgccagtc gagcgccggt gagaattcag ctgggtgggg ccgtgaccaa
     2821 aggacttgga gccggagcca atccggaaat cggacggcgc gcggctttgg aagatcagga
     2881 gaccatcaaa gagtacttag ccggctctga catgattttt attaccgccg gcatgggggg
     2941 tggtacggga accgggggcg caccggtcat tgccagggtg gcgcgtgaag tcggcgcatt
     3001 gacggtgggt gtggtcacga agcccttcat attcgaaggt aagaagcgga tgcggcaggc
     3061 agaagaaggc atcgccgagc ttaagagtag cgtcgatacc ctgatcgtta ttccaaatca
     3121 aaggctactg agcatcgccg ccaagacgac gaccatgcta gaggcgtttc ataaagcgga
     3181 cgaagtgctg ctccaagccg tcagaggtat ctctgatctg attatcacgc ccggcttgat
     3241 caatcttgat ttcgctgacg tcagaacggt catggcggag atgggcttgg cactgatggg
     3301 cgcatcaacc gctaccggtg aaaatcgcgc tatcgaagcg gcgcagaggg cgatatcgag
     3361 cccgctgctc gaggatatct cgattcaagg cgcacgcggc gttctgatca acatcaccgg
     3421 cgggcccgat ctctgtttac atgaggtgaa tgaggcggct tcaatgattc aggaagaggc
     3481 gcatgatgag gcgaatatta ttttcggcgc ggttatagac gagacaatgc ttgatgagat
     3541 ccgcatcacg gtgattgcaa cgggtttcgg agaaggcaag gaagaccgca agccggttat
     3601 cacaagttcc ggtcatgtct caaccatgaa cactttcaat agcatgaacc acgctaacaa
     3661 cagcgtgccc aatgtggcga acgtcaatcc gatcaaaaac aagaaagtcg tacatctcgg
     3721 tactatcgtc gacgatctcg acgcgccgac gtggcagcgc aagaagcccg gcagcgagga
     3781 agtggaaact gtgacgctca gcaagagcac cctgcagttg agcaccggac aagaggacga
     3841 tgacaagtat gacgttccga cattcttgag acggcagatg gactgaacgg acccgctcgc
     3901 gggctggtta aaagataagc tctacttctg cggtgcggct gcgctcggcg gtcgaagact
     3961 cgtccgtcat cggcccgcat ctcgtcaggt cttgtactcg cattgtctgt ggcgatgctg
     4021 cactgtaaag tccgccactt tgaaaatgtg cggattctca gtgtgctctc tcgtctttgg
     4081 ggagtaaact ttctgagact tggtcttcga aaacattaaa cgggcggctg ccgggatcct
     4141 tgcccagcgt ttactgcgcg attgtgagaa ttagacgcta gttcgaagat tttgctcctg
     4201 ccggaaacgc tgcagagcaa gttggatgag ccggtcgatc agcttggggt agggaatccc
     4261 gcttgcctcc cacatcttgg gatacatact gatttgggtg aaaccaggga ttgtgttgat
     4321 ctcgttgacg agaacctgac cattggcttt aacaaaaaaa tcgactcgcg ccattccttc
     4381 gcagcagagt gttttgtagc tggtgattgc aatatcctgg acggatctaa cgatggcttt
     4441 gggtaatttt gcggggatct ccaagcgcga cccccgatcg tcgatgtact ttgcatcgta
     4501 ggagtaaaaa tcgtaacccg ttacgatttc tcccggtagc gacgcgatgg gattgtcgtt
     4561 acccaaaacc gaacactcga tttcgcgccc ttcgatgaat tcctcgatca agattttatt
     4621 gtcaaaacga aatgctgttt ttactgctgc ttccagttcg gaccggctct ctactttgct
     4681 gattccaacc gacgagccga gattggcggg cttgacgaaa agcggctggc ccagctcttt
     4741 tttcagccgg gcaaagttaa tccggtccgc ggtgctgcgg ccgtagactt gaaatcggcc
     4801 gatcggaact ctcgcatcac ggagcagccg tttcatcaca tctttatcca tgccgattgc
     4861 ggatcccaag acgctagctc cgacaaacgg caggttggct aatttgagca aaccctgaac
     4921 ggtgccatct tcgccaaaag gcccatgaag gactggaaac acgacgtcca ccccgagact
     4981 agcaccgcca gagcgaacta aactggattg ttttgcgccc ggtaaaatcg ccgtcaactg
     5041 cgcatcattg aacggcatga cttgcttttg ttcgtcacgc agcagaccag cgccgttgtc
     5101 gtaagaccaa cggccttgcc tatcgatgcc gatggcgatg acctcatact tactcttgtc
     5161 cattgcggcg acgatattgc gggcagaaat tagcgaaatt tcgtgctcgg cagatcggcc
     5221 gccgaagaga atggcaaccc gcaacttatt tttcatgaag agtcaggtac agaaagcgag
     5281 ccccagtacg tgacccgcat cgatgtgatg gagagctgga tgagttatga tttcttagcc
     5341 tgcggggcct gctgctcgat aaccttgacc actttcccgg gatagtttct cttctcgaac
     5401 tgaaggacaa agtatttggc cttcacaggg tcgcctttgt tgtcgaaact aatctgaccg
     5461 gttacccctc taaaattttt aagcttccgc actgccgcag aaacttcctc acggctcggt
     5521 tttttgccgc cgttggcttg aagcgtttgt tcaacggcct tgatgccgac ttgtgcggca
     5581 tcatacccgt acatcgcgaa agattcgact tctttaccga aacgctgctt gaacttcttg
     5641 gcaaatgcag cagtctcggg ataggcgtcc ggcggcccgg cgacggttgt atagtaggat
     5701 ccggttacat gctggccggc gattttaacc atctccgagg aatccaaacc atcaggtccc
     5761 atgaacaccg ccttcacgcc tttttcacgc atctgtttta gcagcaaccc accctgatga
     5821 tagatgccgc cgaagtagac caagtccgga tttctcgctt tcaatggtat aatcatcggg
     5881 gcaaagtttg cccgctcttc agtgccgtcg tagcccaaga cattgagtcc cagtttcttg
     5941 gcttcgttgc ggaagttgtc cgccacgccc tgaccgtaca gtgtcttgtc atgaataata
     6001 tagaccgatt tgagtttaag gtcctgggcg gcaaagcggg cgcctacagg tccctggaca
     6061 tcgtcgcggc cgcaaacgcg attgacattg gcatagccac gatcggtaat ctcagttgct
     6121 gtgttggccg gcgagatcat ggcgagcatc gcgtccttat agacttcgga tgcaggcagc
     6181 gcgaccccgg aattgaaatg gcctaccagc acaaggacat cgggatcggc gaccaggttt
     6241 cttgcattgg cgacgccgac ttctggtttg gcttggtcat cgtacggaac aaacactagg
     6301 tcgtagccca tcaccttgat tgacttcgaa ccttcttcaa ccgctagctg tgcaccaagc
     6361 ttaatgtgct cacccaaagc ggcttgttca cccgatagcg gcgcttgaac ggcaattttg
     6421 atcgtgccct tctgggctgt agcggaaacc ggaatgccca agagcagcaa aaacaacagc
     6481 aacaaagctc gacggggaat cacgatgatg tgtccctcct tttggcgaaa tttacgcgat
     6541 caacacgcgc cagtcaatgc gaatctgggc tcgtgctcgc gctctcagtg attgacactc
     6601 gaagagaagc ggggttaggt tgataaccca tgattatcag cgtcggcatt gacttggttg
     6661 aagtggaacg gattcgcgcc gccttggaac accctcggat cggcctccga tttcgcgaac
     6721 gggtctatac gcagcgagaa attgcttact gcgagagaag acaacgaggc cgcttccaaa
     6781 gttacgccgg ccgtttcgct gccaaagaag ccgtcatgaa ggcgctgggt cggggctggg
     6841 gagcgcaggt gcgttggctc gatatcgaag ttgctcgcgc tatcagcggc aaacctgata
     6901 tcgtcctgca tagtaagacc gcacagctag ccgagaaact cggcatacaa cagtgggccg
     6961 tgtcgatcac tcatactgat caacacggca tggcgtatgt catcgctcag gatgagcgaa
     7021 gataatcgag agtcaccaag caccggcaga gcgaacacat ggcggaactt acgcgccggc
     7081 aggcttcttg actggcaaca gaaacagcag gcccaaagcc cagatcccaa agatcaatcc
     7141 gccgatgctc cacggaaccg ttcgcctgcc gctgtgagcc g
//
LOCUS       DASUTG010000091         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_459024, whole genome shotgun sequence.
ACCESSION   DASUTG010000091 DASUTG010000000
VERSION     DASUTG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459024"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF089_02060"
     CDS             <1..593
                     /locus_tag="VF089_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172770.1"
                     /translation="LLIATAKSSFETQDAAVKHTAARVLLLDHMLANYGPETKAVREL
                     LRRTVALRLEAIWPEDRFQHANFEPHGAAPAATTIERLILQLSPKTDAQRWLQSEALR
                     VGRDIMETRWLVLGGLGSSVPLLFVIVVVFWLTILFGSFGLFAPRNVTVIAVLFLCAL
                     SVSGSIFLILEMDHPFDGLMKVSSAPIRYALSELGR"
     gene            847..1092
                     /locus_tag="VF089_02065"
     CDS             847..1092
                     /locus_tag="VF089_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6172771.1"
                     /translation="MSIPAGARLDSHSHSVINAGVLITGQLTVLTADGKTLDMKAGEA
                     IVEVVNTLHYGINQGDVPAEIVMFYVGKVDTPLPAVK"
     gene            1227..2306
                     /locus_tag="VF089_02070"
     CDS             1227..2306
                     /locus_tag="VF089_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6172772.1"
                     /translation="MRTSFNAILAFALLAGILGLLLLGVGVVMYGRTGGKEIAERLGV
                     PRPAVIAHRGASYFAPEETAPAYLLAREMGVDYLELDLQRTKDGVLIAVHDDDLRRTT
                     NIAEVFHGRETDSVDKFTFAELQSLDAGSWFNLRFPDRARTSFKNVRILRLEDVMNIA
                     EGGFHKPGLYIETKSARRFPGIEKQLIEILTARGWIRPLRSGVAEFPQRQQDRVQARV
                     ILQSFEPDSLALLKELAPHVPAILLIDETMMRKDGWEALLTKAKQHGAGIGTWGYRWS
                     RDPGWSIKDAPRRYLTTWPWHTGQAHRAGLLVHPWTVDERWEMWMVSLFGADGIFTNR
                     PEVALLFYGRADEIDLKPMWERIGY"
     gene            2398..3393
                     /locus_tag="VF089_02075"
     CDS             2398..3393
                     /locus_tag="VF089_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172773.1"
                     /translation="MNKPMKKIIFCAAGLACTILVGYWYASAQSSNAIPDPRPRRFHQ
                     ASNLPDLSGLVWLDGNRFLAVHDAKVEDEIGNPRVSLIELPANSKGVRWLDRTPNFHE
                     LPSNDLESVARIPGTQDVLLVESGDSLGEVGFQRIFKARVIGNSVHIVATSQWPVPIN
                     NVEASAVVALGPLFVFLFAERAQNLSSTELRWALFDPATLRFGSFASVTFANPNPAAF
                     NRPLVAMDVDNDGQIYVAAAFDAEAAGLRDPDNGPFASAVYRIGVVTESLGTPTVQLF
                     ESPLLEGSMDGFKVESIAIREDAEHGFQLFVGLDDENYGATLRFLPEPSQDVSHR"
BASE COUNT          872 a         1081 c         1151 g          890 t
ORIGIN      
        1 gcctgctgat cgccacggcc aagagttcat tcgagacaca ggatgcggcg gtcaaacaca
       61 cggctgccag agtgctgctg ctcgatcata tgctcgccaa ctatgggccg gagacgaaag
      121 cggtgcgcga actgctccgg cgtaccgtgg ctttacggct tgaggcgatc tggccggaag
      181 atcgcttcca acacgcgaat ttcgagcctc acggagcggc gcccgcagca acaaccattg
      241 agaggttgat cctgcaactc tcgccaaaga ccgatgccca gcgctggcta cagtccgaag
      301 cactgcgggt cggccgcgat atcatggaaa cgcgctggct cgttctgggc ggcctgggca
      361 gttccgttcc gttattgttt gtcatcgtgg tggtgttttg gcttacgatc cttttcggca
      421 gttttggtct ttttgcccct cgcaacgtga ccgtcatcgc ggttctgttc ctgtgcgcgc
      481 tatccgtttc cggctcgatc tttcttattt tggagatgga ccacccgttc gacggcctga
      541 tgaaagtgtc gagcgctcca atccggtacg cgctcagtga actgggccgg taggagattg
      601 aacatgttcg cgacgatttt gttgttgtgc ctgttgcgct ctcgatgatt aaaaaggccg
      661 ttagaatccg cacaagcatt tccatcgttc ttctccttca atctggctgc gcgacggttc
      721 aaagaacgca ggaggcgaac gggacctcga tcgtcgtcga ggagctcgtt aaaaacgact
      781 aagagctggg acggccagtt attgcttgcg tatccgcaag gtcagcccgg gatcacaatt
      841 cgacggatga gcatacccgc cggagcccgg ctcgatagtc acagtcattc ggtgatcaat
      901 gccggtgttc tgataaccgg ccaacttacg gtcctgacgg cggacggaaa gacgctggac
      961 atgaaagcag gagaagccat cgtcgaagtg gtgaacacgc tgcactacgg gattaatcag
     1021 ggagatgttc ctgcggaaat tgtcatgttt tatgtgggta aggtggatac gccgctaccg
     1081 gctgtcaaat agcgatgaga agtatcgccg cgctcgcgcc tgacgtgcaa gcgacatgaa
     1141 gcgcgctggc acgaccgacg atccggccta ccaagaaacg atgatggtcc tctatcgact
     1201 cgattcgctc accaagaaag cagacaatgc gtacctcttt taacgccatc cttgcgttcg
     1261 ctctcttggc tggaattctc ggtttgttac ttctcggagt cggcgttgtc atgtatggcc
     1321 ggaccggagg aaaagaaatc gcggaaaggc ttggagtccc tcgtcccgct gtcatcgcgc
     1381 accgcggcgc ctcctatttc gcccccgagg aaaccgctcc cgcgtatctg ttagcgcgtg
     1441 agatgggtgt cgattatctg gaacttgacc ttcagcgaac caaagacggc gtcctgatag
     1501 cggtccacga cgacgatctt cgtcgaacca ccaacattgc cgaggtgttt cacggtcgcg
     1561 agacggacag cgtcgacaag ttcacctttg ccgagctgca aagcctcgat gccggatcgt
     1621 ggttcaacct gcgattcccg gatcgcgcca gaacatcctt taagaacgtc aggattctcc
     1681 gcctcgaaga tgtgatgaat atagcggaag gcgggtttca taagccgggc ctgtatatcg
     1741 agaccaaatc ggcgcgacgc ttcccgggga tagaaaagca gcttattgag atcctcacgg
     1801 cgcgcgggtg gatcaggccg ctgcgcagcg gtgttgccga atttccacag cgacaacagg
     1861 accgggttca agcaagggtc attctccaat cttttgaacc ggacagttta gctctactga
     1921 aagagttagc accacacgtt ccagcgatcc tcctgatcga cgaaaccatg atgagaaaag
     1981 acgggtggga ggcgctcttg actaaggcca agcagcatgg agccggcatt ggcacatggg
     2041 gataccggtg gtcgcgagat cccggctggt ctattaagga tgcgcccagg cgttatctca
     2101 caacctggcc ctggcatacc ggccaagcgc atcgagccgg gcttctcgtg catccatgga
     2161 ccgttgatga acggtgggag atgtggatgg tgtcgctctt tggggccgat ggaatcttta
     2221 ctaatcggcc ggaagtggca cttctctttt atggcagggc agatgagata gatctcaagc
     2281 ctatgtggga gagaatcgga tattgaaatt gcctggacgt tctgagccaa cccaaggcca
     2341 tgtccggtca gaacccggcg ctcaatcgct gagaagtttg gcagcaaagg aaatccagtg
     2401 aacaagccca tgaaaaaaat aattttctgc gccgcaggac tcgcttgcac gatcctcgtt
     2461 ggctattggt acgcttcggc tcaatcatcg aacgccattc ccgacccgag gccgcgcagg
     2521 tttcatcagg cgtcgaatct tcccgacctc agcggtctgg tatggctcga tggcaatcgg
     2581 ttcctggcgg ttcacgatgc caaggtcgaa gatgaaattg gcaatccgcg cgttagcttg
     2641 atcgaattgc cagcaaactc gaaaggagtg cgttggttgg accgaacacc aaacttccac
     2701 gaactcccga gcaatgatct cgagagtgtg gcgcgcatcc ccggaacgca ggacgtgctg
     2761 ctcgtcgaaa gcggcgacag ccttggtgaa gtcggttttc aacgaatctt caaagcgcgc
     2821 gtgatcggca attcggtgca tatcgtcgcg acatcccagt ggcccgtgcc catcaacaat
     2881 gttgaagcca gcgcagtggt ggcgcttggg ccgctttttg tatttctctt cgccgaacgt
     2941 gctcaaaatc tctctagcac cgagttgcgg tgggccttgt ttgacccggc cacgcttcgc
     3001 ttcggttcgt tcgcctcagt aacattcgcg aatcctaatc ccgccgcttt caaccgaccg
     3061 ctcgtcgcca tggatgtgga caacgatggc caaatctatg tcgctgccgc ctttgatgcg
     3121 gaagcggcgg ggctgcgcga cccagataac ggcccgttcg caagcgcggt ctaccgcatc
     3181 ggggtggtga cggagagctt gggtactccg acagtccaac tttttgaatc tcccttgctg
     3241 gaaggtagca tggacggttt caaagtggag tcgatcgcga tccgcgaaga tgcagaacat
     3301 ggatttcagc tctttgtcgg gctcgatgac gagaattatg gtgctacgtt gcggttcctg
     3361 ccggagccct cacaggacgt gtcgcaccgg tgatcgtcgc acggtcggga cacacacgcc
     3421 tctaggctct gtttggtcga agcgtaagct ggccaatcgg cgctgtcggt tcactgtgga
     3481 attgttccct tggcgccgcg acatcggacc tgcttgggta ccaatgttcg cgagcgtgag
     3541 aatctgaccg ggttgatttt tcagcgatga caaaggacac ccttcgccgg agcgtaatcg
     3601 caggactatt cggctgtgca acgttgtctg ttcgtggcac aggtcatgct gattctcaat
     3661 ccaaccgctt tggaatggga agggcggagg catggatgcc caaacagcga tcacttcaaa
     3721 ttcaaatcct ttagaaccag ccgacccgga tgatcccggt gaaactgaaa acatctgtta
     3781 ccgatatcaa ctcgacaggg tcttggagag ccagataacc acccggcgaa gtaaaacaca
     3841 tggcgaatgc gttctttgag gaaagacatc gctcggctta aaacggaggt aaatgagaat
     3901 gaatattaag gttttcagaa gggcggcggc tattgttacc ctcgcgctgg cgataccggc
     3961 cggtagtgcg aaactgtacg ggatcgacgc caac
//
LOCUS       DASUTG010000092         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_15044, whole genome shotgun sequence.
ACCESSION   DASUTG010000092 DASUTG010000000
VERSION     DASUTG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15044"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF089_02080"
     CDS             <1..226
                     /locus_tag="VF089_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172774.1"
                     /translation="LLSMPDALDQLKFAKENGACGIFMRPLEGTRQITDPYFFPLYEQ
                     AQKIDFCIGLHQANGSAAAVDMMVNPQWQP"
     gene            276..644
                     /locus_tag="VF089_02085"
     CDS             276..644
                     /locus_tag="VF089_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172775.1"
                     /translation="MNAGIPKMFPKLRFGFIETAASWVPWVIYELRRRLDTRNSKLPD
                     DILERSGIFVTCQIGDDVPYLIKSCMGENTLMIGTDYGHADSSTELSALSTLKESGGI
                     SSEMHRKIVEDNPRKFYGLL"
     gene            779..1630
                     /locus_tag="VF089_02090"
     CDS             779..1630
                     /locus_tag="VF089_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6172776.1"
                     /translation="MATKLRKLKVFTILFMFLAMIAAREIRAAAAVETQGGKFAGMVN
                     IPAGEFTMGRDSGPKDETPAHKLFLPAFYIDRNLVSAREYAKFIQEKGPTGPKSEMYL
                     DVADPDALVHNQGGKWLPKEGFENHPVGEMSRYGAAAYCAWAGKRLPSEAEWEKAARG
                     TDGRLYPWGNDMPRPDLAFIGGFRGQTVPVGQFPKGASPYGVLDMAGQVWEWTRSMYK
                     PYPYNPKDGREEPAPTDTVVARGGHSASGPEGLTSTSREAVDAGRAAPGHAYIGFRCV
                     SDAQLVL"
     gene            complement(2067..2801)
                     /locus_tag="VF089_02095"
     CDS             complement(2067..2801)
                     /locus_tag="VF089_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX6172777.1"
                     /translation="MAQEKIDATPRQTNLPNESDEYLSKREELRLAEIELMRQRERVA
                     ELRRGLPQGAAIQDYGFEEGPADLDAGDTPIRTVRLSELFSAPNRSLVVYQFMFGKKN
                     TTPCPMCTLWIDGWNGVAHHLAQNVDVAIVAAADPIALRAHARARGWHKLRLLSCGAN
                     TFKYDLGSEDREGNQDSTISVFTRDTDGTLRHFYSVHPSFSSDIQERGIDLLSPVWHI
                     LDLTPQGRGEWYADLGYGTNVSAADR"
     gene            complement(3094..3447)
                     /locus_tag="VF089_02100"
     CDS             complement(3094..3447)
                     /locus_tag="VF089_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172778.1"
                     /translation="MYLDIVDFGGTSGCEKYFSQKDNGFLDDAFGKEREIVREPSVDP
                     AFERANPDDSFGSQQQRHTGTVRFVGSRTVENTSRSREISPWRSSISSIAIRSAPADH
                     LRKRLDIHGLAQVHD"
     gene            3574..>4521
                     /locus_tag="VF089_02105"
     CDS             3574..>4521
                     /locus_tag="VF089_02105"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6172779.1"
                     /translation="MLSREDNEFACRVGPGTPMGELFRRFWLPAMLPDELPKADCPPV
                     RLRLLGEDLVAFRDSNNKVGVVEAHCPHRGASLYFGRNEECGIRCVYHGWKFDVAGNC
                     VDMPSEPPESNFKDKIHITSYPTALHGGLIWVYMGPEHLRAELPRLEWTQVPDSHRSV
                     SKWFQETNYLQGYEGDIDSSHASFLHTYLDPAASPSNDGSAIDPRLKALDRAPKIIVN
                     DTDYGFRYGARRTIGEGTYNWRVTQALLPTYSLIPFIRFPAGGRAWIPVDDNRTMTFY
                     MSYHPDRPLVDQDLAMRRTGRAFPPELIPGTFIPKRNMKN"
BASE COUNT          992 a         1306 c         1240 g          983 t
ORIGIN      
        1 gctgctgagc atgcccgacg cgctcgatca attgaaattt gccaaggaga atggcgcgtg
       61 cggaattttc atgcgcccgc ttgaagggac gcgccagatc actgatccct atttcttccc
      121 tttgtatgaa caggcgcaga agatcgattt ctgcattggg ctgcaccaag ccaatggcag
      181 cgcggcggcc gtcgacatga tggtcaaccc ccaatggcag ccgtgacttt ttcaatcagt
      241 accgaatctt taatgtcgga gcattatttc gcatcatgaa cgctggcatt ccgaaaatgt
      301 ttcccaagct tcggttcggc ttcatcgaga ccgcggcgtc atgggtgccc tgggtaattt
      361 atgagttgcg tcgccgtctc gatacacgaa atagcaagtt gccggatgat attctggagc
      421 gctccggcat ttttgtaacc tgtcagatcg gcgacgacgt gccgtatctg atcaagagct
      481 gcatgggcga gaataccctg atgatcggca cggactacgg ccacgccgat tcttcaaccg
      541 agctatcggc gctctcgaca ttgaaggaaa gtggcgggat cagttccgag atgcaccgaa
      601 aaatagtcga ggacaacccg cggaaatttt acggcttgct gtgaagcaga atgcttattg
      661 ggtatcgatg ggagtctcat cggaacccta cagcctgaca ccaacaattt aggatagttt
      721 tcatgcggag atttcttgcc gcttgccatc gaacatttcc ccatgttaga gcgcagcgat
      781 ggctacgaag ctgcggaagt taaaagtttt cacgattttg tttatgttcc ttgccatgat
      841 cgctgcgcgg gaaattcgcg ccgcggcggc agtggagact caagggggaa agttcgctgg
      901 catggtcaac attccagccg gggaatttac aatgggccga gacagtggcc caaaagatga
      961 aacgccagcg cacaaattgt ttctcccagc cttctatatc gatagaaatc tcgtcagcgc
     1021 cagagagtac gcgaagttca tccaggagaa gggtcccacc gggcccaaga gtgaaatgta
     1081 tttggatgtc gcggatcccg acgccctggt ccacaatcaa ggtggcaagt ggctgcccaa
     1141 ggaaggtttt gagaatcacc ctgtcggtga gatgagccgg tacggcgccg ccgcctattg
     1201 cgcgtgggcc ggtaagcggc ttcccagcga agcggaatgg gaaaaagccg cgcgtggaac
     1261 cgacggccgg ctctatccct ggggtaatga catgccgcgc cctgatttgg cgtttatcgg
     1321 cggctttcgc ggtcaaaccg tccccgtcgg gcagtttcct aaaggagcaa gcccctatgg
     1381 ggttctcgat atggcaggac aggtttggga atggactcgt tcgatgtata aaccctatcc
     1441 ctataatcca aaggacggac gcgaagagcc ggcgcccacc gatacggtgg tcgcccgcgg
     1501 cgggcattca gccagcggtc ccgagggact tacgtccaca tcccgcgaag ccgtcgacgc
     1561 cggtcgcgcc gccccgggac acgcctacat aggattccgt tgcgtatccg acgcgcagtt
     1621 ggttttgtga aaattttccc agcacgattc ccgatacatc tttcaacctc aaccctggat
     1681 gttccgccta ctctcgcctc cagtcttacg ccgcatgcct attccaagcc gctgcacgga
     1741 gctttaccgg cagatcgtag aagaatatcc cgactcgccg tacgagaatc aggcgaagac
     1801 tcgtttggct gagttggaga aagctcgtta gatattcatc aaggacactg ccgcactgct
     1861 ctcgctctaa cggttggctc tcgaattgtt ttcctttgag gagcttttcg gcctgaaaac
     1921 gactgcgcgc ccccgattgc gcgccgccgg aagcctgccg tcaggtttat cgaacggtca
     1981 aaggatgcgc tccgcccaaa tcattagagt taatgccgat tcaagctcca aatgttcagt
     2041 ctgcgtggcc cgtggttcga cgacaatcaa cgatccgccg cactcacgtt cgtgccgtag
     2101 ccaaggtccg cgtaccactc accgcgcccc tgcggagtga gatccaagat atgccaaacg
     2161 ggagacagca gatcgatacc gcgctcttgg atgtcgctcg agaaagacgg atgaactgag
     2221 tagaagtggc gaagggttcc gtcagtatct cgagtgaaaa cggaaatggt cgaatcctga
     2281 ttcccctcgc ggtcttcgct gccgagatcg tatttgaacg tgttggcgcc gcaactcagc
     2341 agcctaagct tgtgccagcc gcgcgcgcgt gcatgtgctc gcagcgcgat cggatcggcc
     2401 gcggccacga tcgcaacgtc gacgttctga gccagatgat gagcgacacc attccaacca
     2461 tcgatccaga gggtgcacat tgggcacggc gttgtgttct tctttccgaa cataaactga
     2521 tagacgacca acgaacgatt aggcgcgctg aacagttcgc tcaggcggac ggtgcgaatc
     2581 ggcgtgtcgc cggcatcgag gtccgccgga ccctcttcga atccataatc ctgaatcgcc
     2641 gcgccttgcg gaagcccgcg gcgaagttct gctacccgct ctcgctggcg catcagctca
     2701 atttccgcca gtcgaagttc ttctcgcttg gacagatatt cgtcggattc gttcggcaaa
     2761 ttggtttgtc gaggcgtcgc atcgattttc tcctgtgcca tggttcatac ctccttaagc
     2821 ttttcgccaa ataggtttag agacattgct gttacatttg cccgccagac accgaatgac
     2881 tcactctata gcgcgacgaa tgagcgagcg cttttaggac agtcgtgctg aaaatttttc
     2941 cgccttttat caaagcatgt cagatcgaag gggaacctga agtatgaact aaaagaatct
     3001 tgcgaacttc tcgcttcgct tcttcgccgg ctgcggattg cgcgcatcac gccgaaagag
     3061 ctcacggaca aactcacggc caaagaaaat atttcaatcg tggacttgcg ccagcccatg
     3121 gatgtcgagg cgtttcctca gatgatccgc gggtgcgctt cgtatagcga tggaagagat
     3181 cgaagagcgc cacggagaga tttctcgcga tcgcgacgtg ttctctactg ttcgtgaccc
     3241 aacgaagcga acagtgccag tgtggcgctg ctgctgcgag ccaaaggaat catcagggtt
     3301 cgcccgctcg aaggcgggat cgacgcttgg ctcgcggaca atttcccttt ccttaccgaa
     3361 agcgtcgtca agaaacccgt tgtccttttg agagaagtat ttttcgcagc cgctggtacc
     3421 gccaaagtcc acaatgtcta aatacaagat ctaatcccaa tccgcttgcc tcctatccgc
     3481 ttgcatcaac tctacgacga ttgacaccct cgccgattca cggataaatc aatgctgaaa
     3541 aatgtttgga attttccata ccgaggaggc gctgtgttaa gcagagaaga caacgaattc
     3601 gcgtgtcgtg tcgggccggg aacgccgatg ggcgagctct tccggcgatt ctggctgccg
     3661 gcgatgctgc ccgacgagtt gcccaaggcg gattgcccgc ccgtgcgcct gcgcctgctc
     3721 ggtgaggatc tggtcgcctt tcgcgactcg aataacaaag tcggtgttgt cgaggctcac
     3781 tgcccgcacc gcggcgcctc gctctacttc gggcgcaacg aggagtgcgg cattcgctgc
     3841 gtctatcacg gctggaaatt tgatgtcgcc ggcaactgcg tcgacatgcc gtccgaacct
     3901 cccgaaagca acttcaaaga caagattcac atcacgtctt atccgacggc gctccacggc
     3961 ggcctgatct gggtgtacat ggggcccgaa catctccgcg cagagctacc tcgactggaa
     4021 tggacccaag tccctgatag ccatcgctcg gtgtcgaagt ggttccagga gaccaactac
     4081 cttcaaggct acgagggaga catcgacagc tcccacgcgt cgttcctgca cacttacctg
     4141 gacccggcgg cttcgccgag taacgacggc agcgccatcg atccaagact gaaagcgctg
     4201 gacagggcgc ccaagatcat cgtgaacgac accgactacg gcttccgcta cggcgcccgt
     4261 cgcacgatcg gcgagggaac gtacaactgg cgggtgactc aggctttgct accgacctac
     4321 agcctcatcc ctttcatccg cttcccggcc ggcggacgag cctggattcc ggtcgacgac
     4381 aatcgcacca tgacctttta tatgtcctac catccggatc ggccgctggt tgaccaggac
     4441 ttggcgatgc gccgcaccgg tcgcgcgttt ccccccgagt tgatccccgg cacgttcatc
     4501 ccgaagcgca acatgaaaaa c
//
LOCUS       DASUTG010000093         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1645699, whole genome shotgun sequence.
ACCESSION   DASUTG010000093 DASUTG010000000
VERSION     DASUTG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645699"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VF089_02110"
     CDS             complement(<1..968)
                     /locus_tag="VF089_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6172780.1"
                     /translation="MQGSSSASAIDRIAFIGNYLPRQCGIATFTTDLCEAIAAEHNHT
                     TCIAVPVNDIEGGYAYPSRVRFELVEKDIDSYRRAADFLNINSVDLVCLQFEYGIFGG
                     LAGSHILALLRELRVPIVTTQHTILREPDPHQRRVLEEVAALSDRLVVMSQRGADFLE
                     TIYRVSPEKIDLIPHGIPDVPFVDPSFHKDVFGVEGKTVLLSFGLLSANKGIENVIAA
                     LPAILAKHSNVVYFIVGATHPHVKRQDGETYRLSLQRLALDKGVAGHVIFYNRFVSME
                     ELVEFIGATDIYITPYLNPAQIVSGTLAYTVGAGKAVISTPYWHAE"
     gene            complement(1346..1654)
                     /locus_tag="VF089_02115"
     CDS             complement(1346..1654)
                     /locus_tag="VF089_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6172781.1"
                     /translation="MGSKLYVGGLPYSVTEGRLQELFSAHGTVESANVISDKFTGQSR
                     GFGFVEMGSSGEAQKAISALNGTQMDGRTLTVNEAKPQARRDGGGGGGGNRDGGRNRW
                     "
     gene            complement(1983..2171)
                     /locus_tag="VF089_02120"
     CDS             complement(1983..2171)
                     /locus_tag="VF089_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172782.1"
                     /translation="MGNQTYQKRQKELARSQKKKDKFARRIQRKTEKVKGGPPLEDDP
                     MTGDRMTSDPSLQGSPAV"
     gene            complement(2242..2685)
                     /locus_tag="VF089_02125"
     CDS             complement(2242..2685)
                     /locus_tag="VF089_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6172783.1"
                     /translation="MKKYKKIPQVGSVMTPFPYSVDTDDTIAEVERLMDEQQIRHIPV
                     QQDGRVVGIISERDVYRLLERFLPQAQKEQIRARDIMIADPYVVSFDAPLNEIALEMA
                     KRHIGSVIILHHEKLAGIVSATDICRILAEIFEAEFSDSTGDDVA"
BASE COUNT          727 a          830 c          790 g          715 t
ORIGIN      
        1 tcctcggcat gccagtatgg ggtggagatt accgccttgc ccgcgcccac ggtataagca
       61 agagtacctg aaacgatctg cgccgggttg agataaggtg tgatgtagat atcggtggcg
      121 ccgatgaact cgacaagctc ctccatacta acaaagcggt tgtagaaaat gacgtgaccg
      181 gctacgcctt tgtccagtgc caagcgttgc agcgacaatc ggtaggtctc gccgtcctgc
      241 cgtttgacgt gaggatgagt tgcgccgacg atgaaataga caacgttcga atgtttggcc
      301 aatatagcgg gaagcgccgc gatgacattt tcgataccct tgttcgccga aagcaagcca
      361 aaactgagca aaacggtttt accttcgacg ccgaaaacat ctttgtgaaa actcggatcg
      421 acaaaaggca catcgggaat accgtgcggg atgagatcga tcttctccgg tgacacgcga
      481 taaatcgtct cgagaaaatc ggcgccacgc tggctcatga ccaccaggcg atccgacagg
      541 gccgcgactt cctccaaaac cctgcgttga tgcggatccg gttcacgcag aatcgtgtgt
      601 tgagtcgtca caatcggaac ccgcagctca cgcagtagcg ccaggatgtg gcttcccgcg
      661 agtccgccga aaatcccata ttcgaactgc aaacaaacca agtccacgct gttgatattc
      721 aagaaatcgg cggcgcgccg gtacgaatca atatctttct ccaccagctc gaacctaaca
      781 cgtgacggat aggcatagcc gccttcgatg tcattaaccg gcacggcgat gcaggtcgtg
      841 tgattgtgtt cggcggcaat cgcctcacac aaatctgtgg tgaacgtggc aatgccacac
      901 tggcgcggca gatagttacc gataaaagcg atgcggtcga tagccgacgc gcttgaactc
      961 ccttgcataa acaacctctt ttttctgacg gccgcgactc tgtggcggtt tgtacggcgc
     1021 taaaaagaca aagaggcggt accgtaggaa ccgcctcttt gaaactcagc cgaaattgga
     1081 ccgagcgaat tctatgtgga cttcggtttg ccagtagtta cttcgctaat gactataaca
     1141 gaccggggca ataaagatac ccttcattta aactaaactc ggtgcgctgc tagtgcgagg
     1201 agatagccct cgcgcgtggt gcaagcacat cacctcacgc gccaaaccat acttgatcgg
     1261 ccgggcaaag tcaggcggac ggacgaaaaa aggggagcaa cccgacctgt tgctccccta
     1321 tctggctcct tagcgtagag aatatttacc agcggttgcg tccgccgtcc cggttgcctc
     1381 cgccaccacc gccaccgtca cgcctggcct gtggcttggc ctcgttgaca gtcagagtgc
     1441 ggccatccat ttgtgtacca ttgagggcgg atatcgcttt ttgcgcctcg cccgacgagc
     1501 ccatctccac gaagccgaaa cctcgtgatt gaccggtaaa tttgtccgag atgacattcg
     1561 cggattctac cgtaccgtgc gccgaaaaaa gttcctgtaa gcgtccctcg gttacggaat
     1621 aaggcaatcc gccaacgtat aatttactac ccatgttctc ctcctatgag agagtgttta
     1681 tgtctttgac cctgagaacc gcactgcaaa tgggcagaac atggatgaac tgagattcga
     1741 accacgctcc ggtcggattc tcattcatag attgcttcgc aaggagagag actgatcaga
     1801 ggccttcgcg attataattt tcctcagaag gccatttact gaagaagatg agtcagccta
     1861 tgtataaacc gaattcaagt caaccaagtt ccgagtgcta ttttttagcg gagacagacg
     1921 ttctcgcagc gcagccggcg ttgccggaga aactcgcttt ggttgcagcg accggtcttt
     1981 ccctaaacgg caggagaacc ttgcaggctt ggatcggacg tcattctgtc gccggtcatc
     2041 ggatcgtcct ctagcggcgg gccgcctttt accttttccg ttttccgttg tatccggcgc
     2101 gcaaacttgt cctttttctt ttgtgaccgc gccaactcct tctgacgctt ctgatatgtt
     2161 tgatttccca tagccgcacc atagcagata ccggcatgga tggaaacgtc agtaataaag
     2221 gtctattgcg gcaagcgaac cttatgcgac gtcatcgccc gtgctatcgg agaattccgc
     2281 ctcaaatatt tcggccagaa tacgacatat atcggttgcc gatacgatac cggcaagttt
     2341 ttcgtgatga agaatgatca ctgagcctat gtggcgtttg gccatttcaa gagcaatctc
     2401 gtttagcggc gcatcgaatg aaactacgta cgggtcagcg atcatgatat cgcgagctcg
     2461 aatctgctcc ttttgcgcct gtggcagaaa gcgttcgagc agacgataga catcccgttc
     2521 ggagatgatg ccgaccaccc ggccatcctg ttggacggga atatgacgaa tctgctgctc
     2581 atccatgagg cgctcgactt ctgcaatcgt atcatcggta tcgacagaat agggaaacgg
     2641 cgtcataacc gatccaactt ggggaatctt tttgtatttt ttcataattt ttgctctgtt
     2701 cgggggcaca cctattttac agcgcgtgct cggcgacgct cgccgctgat attctgcccg
     2761 ccgcagccgg cggacggtgg ttaaagttgg cgttcgccgc cagagcaaac tagactgccc
     2821 cgaggccttt cacaaccgtt gaggttgact ccccacttcg agccgagccc attccactca
     2881 aagcggcagg cacgttgttg atcagtctcc actactccaa ggctagctag gtgtctattg
     2941 caagatggac tcaataggtc cacactcaag gtatgcgctt ccggtatcaa ctctacaatc
     3001 cgcaattgat catgagtggg acgggttggt tcccaggaga ctgaagagat tttcagagaa
     3061 gt
//
LOCUS       DASUTG010000094         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_904517, whole genome shotgun sequence.
ACCESSION   DASUTG010000094 DASUTG010000000
VERSION     DASUTG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904517"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1237)
                     /locus_tag="VF089_02130"
     CDS             complement(14..1237)
                     /locus_tag="VF089_02130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6172784.1"
                     /translation="MKRTFDFSARAQLRLGFIVLSLTAMSLCFGRVTSAQTINIDRVV
                     AELEPEIHRTLLAGNIPSASIALVSGDQVIWSGAYGHSNLWARTPAATNTVYLIGSTF
                     KTMSSVALLQLMEQGKFKLDDRVSDHLTDFKIQGEDPQKPITFRHLLTHTSGMPADFG
                     GFPVWGDTVPPTLEEYLRKSLKVTKPPMTSVTYSNMAYTLIGYLVQKISGKPYKQYIQ
                     EHIFTPLEMNSTAFEPRPDMDERLSIPYRVDEKTGAQVGAVRVKASVWPAGLVYGTVL
                     NQANWLIANLNGGKFKDKQIISQATLDQMFTRQHDQFKGRIENIWGNETAGFGLTWWT
                     QVRDGDRYFAHSGSVQGYTAFLLGNRDRKLGFAILTNGNRAHPHLFKLADLAMDLMKK
                     HTAAKASQPASGSVE"
     gene            complement(1259..2656)
                     /locus_tag="VF089_02135"
     CDS             complement(1259..2656)
                     /locus_tag="VF089_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6172785.1"
                     /translation="MSKKFKIANQIVALLALCLLLVVHVSAQTPAAGPAPSAQEITRK
                     VDEYMSAAMRVNRFSGAVLLARDGKRIVSRGYGMANIEHDVPNTPQTVFRVGSVTKQF
                     TGMAIVMLQERGKLSVNDPICKYLSDCPQAWQPITIKHLLTHTAGIPNYTEFPDFMKT
                     AAAETTAADLTNRFRDKPLQFALGEKYAYSNSGYYLLGTIIERASGKTYADFLQENIF
                     TPLGMKQTGYDNPVRIIKNRAAGYARTSDGLINAAPISMSTAYSAGALYSTVEDLLLW
                     DQALYTEKLVSRKSLDEAFTPFKSNYGYGWTIGKRYDRQVIAHGGGIFGFSSYIARYP
                     ADRVTVVVLSNVEGAPSGEIANNLSAIIFGAPYQIPAERKEIQVAAKTLEKYVGQYQL
                     TPQLVLNVTLEEGKLLAQISTQPKLELFAESETVFFFKAVSAQVTFVVSAQGEVTGIV
                     FRQGGTDIPATKVKM"
     gene            2893..>3513
                     /locus_tag="VF089_02140"
     CDS             2893..>3513
                     /locus_tag="VF089_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX6172786.1"
                     /translation="MSTLEGRLAEAQARLTPRRRELVRAILDAADETCFLSARELAKR
                     YRVDATTILRATQVLGYDSYADFSVDLRQHFVTRITPYTVLRAATQEKRSVADHIDHA
                     LEKALDNINTLKSDLDRPRIIELAKLIKHSRRTLVIGLDFAASLAIYLAYGLSSLGFD
                     AEAPTGSTGTIQHKVKLLTAKDVLVAISFGQCLRDTVEAVLRAKKQG"
BASE COUNT          770 a          930 c          974 g          839 t
ORIGIN      
        1 gtggttgtca tgactattca actgagcccg acgcgggctg cgatgctttc gccgcggtgt
       61 gcttcttcat caaatccatt gccaggtctg ccaacttaaa caggtgcgga tgagcgcgat
      121 ttccgttggt cagaatcgcg aagccaagtt tgcgatcgcg attgcccagc aggaaagctg
      181 tgtaaccctg aacactgccg ctatgtgcaa agtaacgatc gccgtctcga acctgggtcc
      241 accaggtcag gccgaagccc gccgtttcat tcccccagat gttttcgatt cgtcctttga
      301 attggtcgtg ttgtcgagtg aacatctggt caagcgtcgc ctgactgatg atctgtttat
      361 ctttgaactt gccgccgttg agattagcga ttagccagtt agcctggttg agcactgtgc
      421 cgtagaccag acccgccggc cagacagagg ctttaactcg cacggctccc acctgggcgc
      481 cagtcttttc gtcgactctg taaggaatcg aaagtcgttc gtccatgtcc ggccgcggct
      541 cgaatgccgt actgttcatc tctaacggag tgaagatgtg ctcctgaatg tactgcttgt
      601 atggcttacc ggagattttc tgcacgagat aaccgattag tgtgtaagcc atgttcgaat
      661 aagtaaccga ggtcatgggc ggcttggtta ctttcagtga tttgcgcagg tactcctcaa
      721 gagtaggtgg cacggtgtcg ccccataccg ggaaccctcc aaagtccgcc ggcattccgg
      781 atgtgtgcgt cagcagatga cgaaacgtga tgggcttctg gggatcctcg ccttgaatct
      841 tgaaatcggt caggtgatcg ctgacgcgat cgtccaactt gaatttgcct tgttccatta
      901 gctgaagcag ggcaactgag gacatcgtct tgaaggttga accgatgaga tagacagtat
      961 ttgttgcggc gggggtccgc gcccagagat tcgagtggcc gtaagcgcca ctccaaatca
     1021 cctgatcgcc tgagactaaa gcgatggatg cggacggaat gtttcccgcg agcaaggtac
     1081 gatgaatctc aggttcaagc tcggcaacaa cacggtcgat gttgatggtt tgggcgctag
     1141 tgactcgccc gaagcagaga ctcattgcag tcaggcttag aacgatgaag ccaagcctga
     1201 gttgagcgcg tgcggaaaaa tcgaaggttc gtttcatatg aatagatcaa ccagcccact
     1261 acatttttac tttggtcgcg gggatatcgg ttccgccctg acgaaacacg attccagtaa
     1321 cttcgccctg cgcgctcaca acaaacgtga cttgcgcgct aactgcttta aagaagaata
     1381 cggtttccga ttcggcgaaa agttcgagtt tcggttgcgt ggaaatctgc gccagcagct
     1441 tgccctcttc gagcgtgacg ttcagtacca actgcggcgt gagctggtat tgaccgacgt
     1501 atttttcgag cgttttcgct gcaacctgga tttccttccg ttcggcggga atttgatacg
     1561 gcgccccgaa aatgattgcc gacaagttgt tggcgatctc tcctgagggt gctccttcca
     1621 cgttgcttaa gacgacaacc gtaacgcggt ctgcggggta gcgcgcaatg tatgacgaaa
     1681 agccgaagat gccgccgccg tgggcgatca cctgccggtc gtaacgcttg cctatggtcc
     1741 agccgtagcc gtaattactt ttgaacggcg tgaacgcttc gtcgagcgat ttccgcgaca
     1801 cgagtttttc agtgtagagc gcctggtccc acaggagcag atcctcgaca gttgaataca
     1861 gcgcgccggc cgagtaggcg gtggacatgg aaatcggcgc cgcgttgatc aaaccgtcgc
     1921 ttgtgcgtgc atagcccgct gcacggttct tgatgatcct aacgggattg tcgtatccgg
     1981 tttgcttcat tccgagcggg gtgaaaatgt tttcctgcaa aaagtctgcg taagttttgc
     2041 cggacgcgcg ttcgatgatc gtgccgagaa ggtaataacc ggagttgctg taagcgtatt
     2101 tttctccgag cgcaaattgg agaggcttat ctcggaaccg attcgttaag tcggcggcgg
     2161 tagtttcagc cgccgcagtc ttcatgaaat cgggaaattc agtgtagttc ggtatgccgg
     2221 cagtgtgcgt caaaagatgt ttgatcgtga tcggctgcca cgcctgtggg cagtccgaca
     2281 gatacttgca gatcggatcg ttcacgctta gcttgccgcg ctcttgcagc atcacgatgg
     2341 ccattcccgt aaactgcttg gtcaccgaac ccacacgaaa aacagtttgc ggcgtattcg
     2401 gcacatcgtg ctcaatgttc gccataccgt aaccacggct aacaatgcgc ttcccgtcgc
     2461 gggccaggag caccgcaccg ctgaagcggt taacacgcat cgccgcgctc atgtattcgt
     2521 ctactttcct cgtaatctcc tgcgcggaag gagccggccc tgcggcgggg gtttgcgccg
     2581 aaacatggac cacgagaagc aggcataacg ccagcagggc gactatctga tttgcgatct
     2641 tgaatttttt tgacatgaaa agcggtgttg cggatcttcg agttgcagct ctgaattgaa
     2701 tatcgatgca tataccgcac atgctgcaca aaagtcaaca acgctataag tgctgcaaca
     2761 aatcttgttt gatgcgcctc gatttgctat cttccctcgc acaaattcgt ccggcgaggt
     2821 aagcagatgg cgtctaactc acacaggaag actcacagca tttctcttgg ttctgacaac
     2881 gctggtggga aagtgtcaac attggaaggc cgcctggcgg aggcgcaagc tcggctcacc
     2941 cctcgacgcc gcgagttggt ccgggccatc cttgacgcgg cggatgaaac ttgtttcctc
     3001 tctgctcgcg aattggccaa gcgctatcgc gtcgatgcta cgacgattct gcgagccacg
     3061 caggtgctgg gttacgacag ttatgctgac ttctccgtag acttgagaca gcacttcgtt
     3121 acgcgcatca ctccttacac agtgctgcgt gcggcgaccc aggaaaaacg gagtgtcgcc
     3181 gatcacatcg atcacgcttt ggagaaggcg ctcgacaaca taaacacctt gaaatcagac
     3241 ctggataggc cgcgcataat agagctggcc aagctgatta aacactcgcg ccgcacactc
     3301 gtgatcgggt tagacttcgc tgcttcgcta gccatttacc tggcttacgg actgtcctcg
     3361 ctcggcttcg acgctgaagc gccgacgggc agcactggaa caattcaaca taaagtaaag
     3421 ctcttgactg ccaaagatgt gctcgtggcc attagttttg ggcagtgcct tagggatacg
     3481 gtcgaggcag tgctgcgcgc caaaaaacag ggc
//
LOCUS       DASUTG010000095         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_756364, whole genome shotgun sequence.
ACCESSION   DASUTG010000095 DASUTG010000000
VERSION     DASUTG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756364"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..581)
                     /locus_tag="VF089_02145"
     CDS             complement(162..581)
                     /locus_tag="VF089_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6172787.1"
                     /translation="MITRMSHATVWVRNQQEALEFYRDKLGFEVDTDAMVGDDFRWLT
                     MRIPSQPGFEIILMEPKPGYILDEESAAQLKALMEKGVLGGGVFETDDIHKTYEELKA
                     KGVEFKGPPTTQSWGTATVMKDNSGNWFSVGQDKTTA"
     gene            complement(754..1167)
                     /locus_tag="VF089_02150"
     CDS             complement(754..1167)
                     /locus_tag="VF089_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6172788.1"
                     /translation="MPEAKAKVGSEVHERLCRARAFINERYHEPIDLDEISREACLSR
                     YHFLRLFREEFATTPHQYLIDRRIEKAKELLRHRRLTVTDVCFEVGFQSLGSFSTLFR
                     QRVGDAPVNYRQRQREAQSKIPTCFLDMYGLGVKD"
     gene            complement(1374..>2724)
                     /locus_tag="VF089_02155"
     CDS             complement(1374..>2724)
                     /locus_tag="VF089_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6172789.1"
                     /translation="DRSLENAMTVTTDALTGEEIVELCRRHTLFEWSAQAAVDPIPMA
                     RARGIYFWTPEGKRYIDFNSQLMCVNIGHGDERVVRAIKEQAEVLPYANPFMATDVRG
                     RLAAKLAELAPGDIDTFFFTNGGAEANENAIRIARLFTGRHKIGARYRSYHGGTAGAL
                     TLTGDPRRWAAEPGIPGVFRIPDFYHGVNKREETAEEVLARTEETIQLEGANTIAAII
                     VEPVTGTNGILVPPDGYLQGLRALCDKYGILLIADEVMSGFGRTGEWFAVDHWKVKPD
                     LLSMAKGLTSAYVQLGAVGMRREIADHFAKNVFYGGLTYNSHPLGCAAALATIAVYEE
                     DQLIDKARKMGVVMSGLLEDLKARHPSVGATRSIGLFGIVELVRNQETLEPMAPFNGS
                     SPEMQALGKYFREAGLYTFVRWNTFFTNPPLCITEAELREAFAIIDKGLETTDRAVG"
BASE COUNT          574 a          778 c          752 g          620 t
ORIGIN      
        1 cgcatgcctc ccggcatgct cttgtggtac gcatgcctcc cggcatgctc ttgtggtacg
       61 catgcctccc gggatgctct tatcagaagc acgccggagg cgtgcgtacc cagttcgcca
      121 gaggcgtgcg tacccggtcg ccggtcgcgt gtgtaccctt gtcaggcggt ggttttgtcc
      181 tgaccgacgc taaaccaatt gcccgagttg tctttcataa ccgtcgcggt tccccatgac
      241 tgagtcgttg gcgggccctt gaactcaacg cccttggctt taagttcctc gtaagtcttg
      301 tggatatcgt cggtctcgaa aactccgccg cccaggacgc ccttctccat cagcgctttc
      361 aattgcgccg ccgattcttc gtccagaatg tatcccggtt ttggctccat cagaatgatc
      421 tcaaagccgg gttgagacgg aatcctcatg gtcagccagc ggaagtcatc gccgaccatc
      481 gcatcggtat cgacctcaaa gccgagttta tcgcgataga actccagtgc ctcctgctgg
      541 tttctgaccc aaaccgttgc atgcgacatt ctcgtaatca tgttagttag tctccttcgc
      601 cagattcgcg gaggagcata cagatttcag cgaaggctgg cttctcgaaa attgctgaaa
      661 tgaagctttg tcggaaccac ctgcgtgagc gggtgggttc gatgttggga tagcacccgc
      721 ccgctgacgc aggcggttcc gacaagatga ttcctaatcc ttcactccga gcccatacat
      781 gtcgagaaag caggtcggaa ttttgctctg ggcctctcgc tgtcgctggc gatagttcac
      841 cggagcatca ccgacgcgct ggcgaaacag cgtgctgaac gagccgaggc tttgaaagcc
      901 tacttcgaaa cacacgtcgg taaccgtcag acgccggtgt ctgagtaact ctttggcctt
      961 ttcaatccgc cggtcaatca ggtactggtg tggagtggtg gcaaactctt cgcgaaacag
     1021 gcggagaaag tgatagcgcg agaggcaagc ctcgcgggaa atctcatcga gatcgatcgg
     1081 ctcgtgatag cgctcgttga tgaaggcgcg cgcgcgacag agccgctcat gaacctcact
     1141 cccaactttg gctttagcct caggcatttt gcgagcggga ttctactgcg ggatagatgt
     1201 aatcggaaac ccgaccggac attcatgaga acatccacag attacgcaga taaagaagat
     1261 aagaaaggga gactcgctac ttatgcagat attgattcaa tgactcattg gttattgatt
     1321 caatggaaaa tgaaccaatg gatcaatcct atctgtgtaa tctgtggata tatctatccc
     1381 accgcgcgat cggtggtctc caaaccttta tcgatgatcg cgaaggcctc gcgcagctcg
     1441 gcttcagtga tacagagtgg gggattcgta aagaaagtat tccagcgcac gaacgtgtag
     1501 agacctgctt cacggaaata ctttccgagc gcctgcatct cgggcgaaga gccgttgaac
     1561 ggcgccattg gttccagcgt ttcctggttc cgcaccagct caacgatgcc gaaaagaccg
     1621 atgctgcgcg tcgcgcccac tgatggatga cgggctttga gatcttcgag cagaccgctc
     1681 atcacgacac ccatcttccg cgctttatcg atcaactgat cttcttcata gaccgcgatc
     1741 gttgctaaag ccgccgcgca gccgagtgga tgactgttgt aagtgagtcc accatagaaa
     1801 acattcttcg caaagtgatc cgcaatttct cgccgcatgc ccaccgcccc aagctgtacg
     1861 taggcgctgg tcaggccctt cgccattgaa agcagatcgg gtttcacttt ccagtgatcg
     1921 acggcgaacc attcgccggt gcggccaaag ccggacatta cttcgtcggc gatcaggagg
     1981 atgccgtact tgtcgcataa agcgcgcagg ccctgcaggt aaccatccgg tggaacgaga
     2041 atcccgttag tccccgtcac cggctcgaca atgatcgcgg cgatggtgtt cgcgccttcg
     2101 agctggattg tctcttcagt gcgcgcgagc acttcttcgg ccgtctcttc tctcttgttt
     2161 actccgtgat agaaatcggg aatgcgaaag accccgggga taccgggctc ggcggcccag
     2221 cgtcgtgggt cgccggtcag cgtcaacgcg ccggccgtgc cgccgtgata cgagcgataa
     2281 cgcgcgccaa ttttgtggcg tccggtgaaa agacgcgcga tacgaattgc gttctcgttc
     2341 gcttcggccc cgccattggt gaagaaaaaa gtgtcaatgt cgcccggcgc taactcggca
     2401 agttttgccg ccagccgccc gcgcacgtct gtggccatga aaggattagc gtaaggcaga
     2461 acttcagcct gctctttgat ggcgcgaacc acacgctcgt cgccgtggcc gatgttcacg
     2521 cacatcaact ggctgttgaa gtcgatgtag cgcttgcctt ccggcgtcca gaagtagatc
     2581 ccacgggccc gggccatcgg aatcggatca acggccgctt gtgcagacca ctcgaagagt
     2641 gtgtggcggc gacagagttc gacgatctct tcgccggtca gggcgtcagt tgtaacggtc
     2701 atagcgtttt ctaagcttcg gtca
//
LOCUS       DASUTG010000096         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1794707, whole genome shotgun sequence.
ACCESSION   DASUTG010000096 DASUTG010000000
VERSION     DASUTG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794707"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF089_02160"
     CDS             complement(<1..259)
                     /locus_tag="VF089_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172790.1"
                     /translation="MLPIQRGSLDIQLAGFVNELLLCKLVEVIGSWLMRWLFPGFTTR
                     SPGRWIACRKRIGQRIVDPRLRTFLTICATAASFFYGRFLYG"
     gene            390..>2399
                     /gene="ligD"
                     /locus_tag="VF089_02165"
     CDS             390..>2399
                     /gene="ligD"
                     /locus_tag="VF089_02165"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6172791.1"
                     /translation="MVSLSVKIVNRKLALSNIDKVLYPETGFTKGQVIDFYSRIGRYI
                     LPHIENRPITLKRYPNGIKGEHFYEKNAPSFTPAWIKTYPIQRSSRESMINYILINDL
                     PTLVWSANLANLEIHPFLAKAPEISVPTMVVFDLDPGDGATLMQSCEVAFLLRDVLDR
                     LALDSLLKVSGSKGIHVHVPLNTRVSYDMTQAFAQSVARYLASEHPKLIVSEMAKIKR
                     TGKVFIDWSQNSEHKSTVAVYSLRAKAERPFVAMPVSWRELEIALDKNDPATLFFEPE
                     AALKRLAKVGDLFESSLRLKQTLPKPFLQLQSQVSSDPSEAGLAALEPYRRKRDFTKT
                     REPAPSVPGISRQGGRRLFVIQKHAASHLHYDFRLEMGGTLKSWAVPKGPPYDLHERR
                     LAMATEDHPMDYANFEGIIPAGEYGGGTVMVWDVGTYELIDGNFWKGKLHVFLNGKKL
                     RGEWVLVKGKDRNGKANVWYLIKAGSAMKRISEKRDNSSALTGRNLGQIAKAEDAVWH
                     SNRTAAQRISTSNGDGTPATDLPDIRIRFIEPMLSETVDELPEDSDLWSYEIKLDGYR
                     CLALRDESGVRLYSRKRNLLNSRFPTIVKALESMEPGMILDGEVVALDNNGRPSFNLL
                     QNFRFTAKQIHFYAFDLLAQRNKNLMGLALEKRRELLALRLENIAD"
BASE COUNT          616 a          607 c          644 g          532 t
ORIGIN      
        1 ggccgtacag gaatcggccg tagaagaaac tcgcggcggt agcgcagatc gtgagaaaag
       61 ttcggagccg gggatcgacg atgcgttggc cgattctttt ccggcaagcg atccaccttc
      121 ctggactacg ggtcgtgaaa ccaggaaaga gccagcgcat gagccaactg ccgataacct
      181 cgaccagctt acagagcaac agctcattga caaagcccgc gagttgaata tcgaggcttc
      241 cgcgctgaat cgggagcaac tgatatcagc gattcgaaat cgatcgtaag tcgatccgcg
      301 acggactatc ctcaagcaat ggcctgcggg tgagcgcgcc gaaagagtgt acaattggca
      361 tctgcccaac ggcgcggtag aatttcttgg tggtttcgct gtctgtcaag atcgtcaaca
      421 gaaaactcgc tctctcgaac attgacaaag tactttaccc cgaaacaggc ttcacgaagg
      481 ggcaggtgat cgatttctac agccggatcg gccgctacat actgccccac atcgaaaatc
      541 gaccgattac gcttaagcgc tatcctaacg gcatcaaggg cgagcatttc tacgaaaaga
      601 acgctccgtc tttcaccccc gcttggataa agacttaccc gatacagcgc agctcccgcg
      661 agtcgatgat caattatatc ctgatcaacg acctccccac gctagtatgg tcggcaaatc
      721 tagctaatct cgagatccat ccctttctgg ctaaagcacc cgagatcagc gttccgacaa
      781 tggtcgtttt cgatctcgat cccggagacg gagcaaccct gatgcaatcc tgtgaagtgg
      841 cttttttatt acgagatgtg ctcgatcgct tggcactcga ttctttgctc aaggtctcgg
      901 gctctaaagg cattcacgtg cacgttcctc tcaatacacg ggttagctac gatatgacac
      961 aagcatttgc acaaagcgtc gccaggtatc ttgccagtga gcatcctaaa ttgatcgtgt
     1021 cggagatggc taagatcaag cgcacgggaa aagttttcat cgactggagc caaaactcag
     1081 agcataagtc gaccgttgcc gtgtattcct tgagggcgaa ggcggaaagg ccgtttgtcg
     1141 cgatgccggt aagttggcgc gagctagaaa ttgccctgga taaaaatgac cctgctacat
     1201 tattttttga accagaggcg gcattgaaga gactggcaaa ggtcggcgat ttatttgagt
     1261 cttcgttgcg cctgaagcaa acactgccaa agccgttttt gcagctccag tcccaggtct
     1321 ccagtgatcc gagtgaggcg ggcctggctg ctcttgaacc gtatcgtcgc aaacgcgact
     1381 tcactaagac tcgggaaccg gcgccttctg ttcctgggat tagccgtcaa ggaggacgcc
     1441 gtctgtttgt catacagaag catgccgcca gtcaccttca ttatgatttt cgactggaaa
     1501 tgggcggcac tttgaaatca tgggcggtcc ccaaggggcc gccctatgat ctacacgaac
     1561 gaaggttggc aatggcgacc gaagatcacc cgatggacta tgcgaacttt gaagggatca
     1621 tcccggcagg agaatacggc ggcggtaccg tgatggtctg ggacgttgga acttacgagc
     1681 tcattgacgg gaacttttgg aaaggaaaac ttcatgtttt tctaaacggc aagaagcttc
     1741 gaggcgaatg ggtgcttgtg aaagggaagg accgaaacgg taaagccaat gtttggtatc
     1801 tcataaaggc cggctccgcg atgaagcgaa tctctgaaaa gagagacaat tcttccgcat
     1861 tgacaggccg aaatcttggc caaattgcca aagcggagga tgccgtctgg cacagcaaca
     1921 gaactgcggc acagaggatc tcaacaagca acggcgatgg gactccggcc acggatctac
     1981 ccgatatacg catcaggttc atagagccga tgctgtccga gactgtcgac gagttgccgg
     2041 aggattctga cctatggagt tacgagataa agctcgacgg ttatcgctgt ctggcgctgc
     2101 gagatgaaag cggcgttagg ctgtactcgc gaaagagaaa tctattaaat tcgcgtttcc
     2161 cgacgatcgt aaaagccctt gaaagcatgg aacctggaat gatcttagat ggtgaggtag
     2221 tggcactcga taataacgga aggccatcat tcaatcttct gcagaacttt cgttttacag
     2281 ccaaacagat tcacttctat gcgttcgacc tgctagctca gcggaacaag aacctcatgg
     2341 gtcttgcgct cgagaaaagg cgtgagttgc tggccctccg gttggaaaac attgccgac
//
LOCUS       DASUTG010000097         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_163951, whole genome shotgun sequence.
ACCESSION   DASUTG010000097 DASUTG010000000
VERSION     DASUTG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163951"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..892
                     /locus_tag="VF089_02170"
     CDS             95..892
                     /locus_tag="VF089_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX6172792.1"
                     /translation="MPSLKIATWNVNGIRAREAQVLEWVERERPDVVCLQELKSTVAQ
                     VPAALCELQGYRCYWHGARAYSGVGLHIRRDAFAQEPRFFHPSFDFETRVVAAQMDEL
                     VVVSAYVPNGGKDFAAKMQFLRNMENYVHEVHAGGARLVFCGDLNIARTERDVHPKER
                     NPGIIGQRPEERELFERILGHGLVDVGRALDPDNENLFTWWAPWRRMRERNIGWRLDY
                     VLASQSLAGRASQCSVQREIGTSDHAPVVAVFDLDGATVATATREPG"
     gene            889..1545
                     /locus_tag="VF089_02175"
     CDS             889..1545
                     /locus_tag="VF089_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6172793.1"
                     /translation="MKKDRATPADLSHFGRLAEYNRKRHFGITTEPQGKLGRRNRKTL
                     EFVVQKHRASRLHYDFRLEHDGVMLSWAVPKGPSTDPANKRFAAQTEDHPIEYNQFEG
                     VIPEGEYGGGTVMIWDRGTWEPEVEDVEKALAKGDLKFKLNGKKLRGSWVLVRMRPRQ
                     WLLIKHRDTAASAEDITVARPRSAVSRRNLAGIARKAGATPRQLAQAEEADKAVAAPE
                     "
     gene            complement(1671..2357)
                     /locus_tag="VF089_02180"
     CDS             complement(1671..2357)
                     /locus_tag="VF089_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6172794.1"
                     /translation="MLQAVIFDVDGTLVDSVDLHAEAWKVAFEKFGKTFPFHEIRRQI
                     GKGSDQLLPVFLSAEELDQFGEDLDEYRSKIFKNEFLPRVQAFPKVRNLFQRIKKDGT
                     QIVLASSAKADELDTYKKIARIDDLIESETSSDDADRSKPYPDIFQAALARLKDVAPE
                     NAVVIGDTPYDAQAASKANLRTIGLLCGGWTKEELRRAGCVAIYLDVEDLLLQYDSQF
                     GSPGATKTKG"
     gene            complement(2806..>3691)
                     /locus_tag="VF089_02185"
     CDS             complement(2806..>3691)
                     /locus_tag="VF089_02185"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6172795.1"
                     /translation="FLVPLLVLVYALMVANWEPGKAGMAAAITAFVVGALGKETRPSP
                     KGILSSVEETGKILLDIVVVTALAGLVIGAFQLSGLTFKFALLLVTTAGGNIVFLLLL
                     TAVVSIILGMSMPTTVVYIMLAVLVGPALVQVGIPPLAAHLFFFYFGMLSLITPPDCI
                     ATYAAASIARADFWKSGWTGMRLGIVAYIVPFVFVFHPALLFKGAIGEIVTAIVTASI
                     GVIFLATGVAGYLFRELGYIKRALLILAGAALIPPASTTFWLLTNCAGIVLGVALVTA
                     EWSSAWKHHVRLANIDQV"
BASE COUNT          871 a          981 c         1048 g          791 t
ORIGIN      
        1 cttcttgccg ccaatgttaa cgtcctaaga tctggtttgt ttggtgattg aagttccagc
       61 ggcctgtttc tatacttccg cactggagaa aattatgcct tcgttgaaaa ttgcgacttg
      121 gaacgtcaat ggcattcgcg cgcgcgaagc gcaggtgctc gagtgggttg agcgcgagcg
      181 cccggacgtt gtctgccttc aggagttgaa gtccaccgta gcgcaggttc ccgccgcgct
      241 ttgcgaattg cagggttatc gctgctactg gcatggcgcg cgcgcttact ctggagtcgg
      301 tctgcacata cgccgcgacg cttttgccca ggagccgcgc ttctttcatc cttcttttga
      361 tttcgaaacc cgtgtggtcg cggcgcaaat ggacgagctg gtggtggtct cggcttatgt
      421 tccaaacggc ggtaaagatt tcgcggcaaa aatgcagttc ctgcgcaata tggaaaacta
      481 cgttcacgaa gttcatgccg gcggcgcgcg gctggtcttc tgtggtgatc tcaacattgc
      541 gcgaaccgag cgcgatgtgc atcccaagga gcgaaaccct gggattatcg ggcaacggcc
      601 ggaagaaagg gaattgttcg agcgtattct tggccacggc ttggtcgacg ttggacgagc
      661 gcttgatccg gataacgaaa atcttttcac ctggtgggcg ccatggcgtc ggatgcgcga
      721 acgcaacatc gggtggcggc tcgattacgt tctcgccagc caatctctag ccgggcgcgc
      781 ttcgcagtgc tcggtccagc gcgaaatcgg caccagcgat catgcgccgg tggtcgccgt
      841 gttcgatctc gatggcgcta ctgtagcaac cgcgacgcga gaacctggat gaaaaaagat
      901 cgtgccacgc ccgctgatct gagccacttc ggcaggctgg cggaatataa tcgtaagcgt
      961 cacttcggta tcacgacgga gccgcagggg aagctcgggc ggcgaaacag gaaaacttta
     1021 gagttcgttg ttcaaaaaca tcgcgcgagc cggctgcact acgattttcg cttagagcat
     1081 gatggcgtga tgctctcatg ggcagtgcca aagggcccct ccactgatcc ggcaaacaag
     1141 cgttttgcag cgcagaccga agatcacccc atcgagtaca accaattcga aggtgtaatc
     1201 ccggagggcg aatatggtgg tggaacggtg atgatttggg atcgcggtac gtgggagccg
     1261 gaagtggagg atgtcgagaa ggcgctcgcc aagggcgatt tgaagttcaa actaaacggt
     1321 aagaagctgc gcgggtcctg ggtgctcgtg cgcatgcggc cgcggcagtg gcttttgatc
     1381 aagcaccgtg acaccgccgc ctctgccgag gacatcacgg ttgcaaggcc gcgctccgcg
     1441 gtatctcggc gaaaccttgc aggaatcgcg cgcaaggcgg gcgcaactcc tcgacagctc
     1501 gcgcaagcag aggaagcgga taaggctgtt gcagcgcccg agtgaagccg tcagtcactg
     1561 gtgcgttttg ctagtgaagt gctggagcgg ctccagccac tcccaaccca ctccagaact
     1621 accaacacct tctttcagga gagcttagcg gagacgtgag agagagaggc ttaccctttg
     1681 gtttttgttg cgcccggaga gccaaactgc gagtcatact gcagcagcag atcttctaca
     1741 tccaggtaaa tcgccacaca gccggcgcgg cgcagttctt ccttggtcca gccaccgcac
     1801 agtaagccaa tcgttcttaa gtttgccttg gaagcagcct gtgcatcgta aggtgtatcg
     1861 ccgatcacga cggcattttc gggcgctacg tccttcaaac gggccagggc tgcctggaaa
     1921 atatcggggt acggcttaga tctgtcggca tcatcggacg aggtttcact ttcgattaga
     1981 tcgtcgattc gagcaatttt cttgtacgtg tcgagttcat ccgctttcgc ggaggaggcc
     2041 aggacgattt gcgtgccgtc tttttttatt cgctgaaaca agttccgcac tttcggaaat
     2101 gcctgcacgc ggggcagaaa ttcgttttta aatatcttac tgcggtattc gtccaaatct
     2161 tcgccgaatt gatctagctc ttccgcggaa agaaaaaccg gcaacaattg gtcggacccc
     2221 ttgccaattt ggcggcggat ctcgtgaaat ggaaacgtct tgccgaattt ttcaaacgca
     2281 actttccacg cctcggcatg cagatcgacg gaatcgacga gtgtgccatc gacatcaaat
     2341 atcacggctt gcagcattat ttttttaact acggtatgtg gtcccgatga gacacttgcc
     2401 ttccactttg tgccaggaac gaaacaggga gcatagcgag ttgcaccatt gagacagacg
     2461 aagcagtaaa tactcgccct gcttcgtctg ccgagagtcg ttcaggtcag cgcaatccta
     2521 aatagcccaa gataaacagc actacgacga tgacgccgat gatatagaaa atatttccag
     2581 gcatgaccga tcctccttag tagttgattt tcctcaggcg atccaccggt ttccgaatta
     2641 tgtggagcgc aacggaatat ttgcattcac aaccgggttg caagcgtgac tagctttgag
     2701 caatcgatat gccatgagag aaatcgatag ttttaagtgt gagcgctgcg agttcggcat
     2761 aggatctaag gtgaacaccg gttttctact agatcgaaac gagaatcaga cttgatcgat
     2821 gttagccagt ctgacgtgat gcttccaagc cgaactccac tccgcagtca caagcgcgac
     2881 tcctaaaacg atgcccgcgc aattggtcag caaccagaat gtggtggatg ccggcggaat
     2941 caatgccgct cccgccagga tcaacagcgc tctcttgatg tagccaagct ccctgaagag
     3001 gtagccggcc acgcctgtgg ctaaaaagat tacgccgata cttgccgtaa cgatggccgt
     3061 cacgatttct ccgatagcgc ctttgaagag caaggcagga tgaaacacga acacgaatgg
     3121 aacgatgtag gcgacgatcc cgagtctcat gccggtccag cctgacttcc aaaaatcggc
     3181 ccgcgcaatc gaagcggccg catacgtggc gatacaatcc ggtggcgtga tcaaggacaa
     3241 catgccgaag tagaagaaaa aaagatgagc cgcgagcgga ggaatgccga cctgaaccaa
     3301 cgccggcccg accaagactg ccagcatgat gtagacgacg gtagtcggca tggacatacc
     3361 gaggatgatt gaaacgaccg cggttaaaag cagcagaaag acgatgttgc cgcccgccgt
     3421 agtgaccagt aacaaagcga atttgaacgt gaggccggaa agctgaaaag cgccgatgac
     3481 gagtcccgca agcgcggtga cgaccacgat atccaggaga attttgccgg tctcctcgac
     3541 gctgcttaaa atgcctttgg ggctcggccg ggtctccttg cccagggcgc caacgacaaa
     3601 tgctgtgata gccgcggcca tgccggcctt acctggttcc cagttggcga ccatgagcgc
     3661 ataaactaat accaacagcg gaaccagaaa t
//
LOCUS       DASUTG010000098         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1350089, whole genome shotgun sequence.
ACCESSION   DASUTG010000098 DASUTG010000000
VERSION     DASUTG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350089"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VF089_02190"
     CDS             <1..1154
                     /locus_tag="VF089_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6172796.1"
                     /translation="PSLGALGPRLLVIAGDQGRPAAMVRNTVASLPEATVVWLPGYFS
                     PPWADVVADCTEQVETAILNILSSEHAKNLRNVQGDVRGEIAGITYHSQGAGSPLVLL
                     PLSLASSQWDPLLARLSGAVCTVTLGGPELGFSAMLESRGLSSGYLSVVKQVIDAVRL
                     KSGELVLEVGCGSGVLDRWLAKYTTRTNPIIGVDVSRYLLREATALARKEGVGDIIAF
                     HNGDAEALPFRENHVDVAFSFTVLEEGNADRMLAELVRVTKPGGRIAVMVRALDIPWV
                     INVPLRPELKTKVEIPHGFVAAEGCADGGLYRRLHAAGLTQVQMFPQFAAFNRPDTAQ
                     AQFAHSAIASSLNPEETQEWRAAVAHAVADGTYFIAQPFHCALGTKPEL"
     gene            complement(1444..1614)
                     /locus_tag="VF089_02195"
     CDS             complement(1444..1614)
                     /locus_tag="VF089_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172797.1"
                     /translation="MPWNEVYYPKSMRNLSREVRLKAIEIANALLEEGYDEGTAIRIA
                     IAKAKEWAERSR"
     gene            2348..2968
                     /gene="pdxH"
                     /locus_tag="VF089_02200"
     CDS             2348..2968
                     /gene="pdxH"
                     /locus_tag="VF089_02200"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX6172798.1"
                     /translation="MSAARIRQRIERNRMNSDPILRFKGLLAEAAQLGIVPYNAAAFA
                     TTDRDLQPTVRMLLLKDVDHCGLVFYTNLESRKARQLSDNPRASACFWWPQLRQQVRF
                     EGAVELVSEREADDYFASRPRGSQIGAWASHQSSELSSRDELMAAAASFSAKYKDRTV
                     PRPPHWSGYRMFAERIEFWREQPDRLHDRELYTRQADGWNIMLLAP"
BASE COUNT          672 a          894 c          863 g          680 t
ORIGIN      
        1 acccgagtct aggtgcactc ggcccccgac ttctagttat tgcgggagac cagggtcgtc
       61 cggcggctat ggtgcgcaat accgtggcaa gtctgccaga agctacggta gtctggctcc
      121 cgggctattt cagtccacca tgggccgatg tggtggccga ctgcactgaa caagtggaaa
      181 ccgcgatcct caatatcctc tccagtgaac acgcaaagaa tcttagaaac gtgcagggcg
      241 atgttcgagg tgaaattgcc ggcatcacct accacagtca aggcgccggc tcgccgctcg
      301 tcctgctccc actgagctta gcgtcctcac aatgggaccc gctgctggct cggctcagcg
      361 gggcagtttg cacggtcacg cttggcggtc ccgaattagg attttcggca atgctggaat
      421 cgcgtggcct ttccagcggc tatcttagcg tcgtcaagca agtaatcgat gcggtgcggc
      481 tgaagtctgg tgaactcgtt ttggaagtgg ggtgtggctc gggagtgctg gatcgctggc
      541 tcgcaaagta taccacgcgg accaatccta tcattggcgt agatgtcagc cgctaccttc
      601 tccgggaggc aacggctctc gcccgaaaag agggagttgg ggacattatc gcatttcata
      661 atggagacgc cgaagctctc ccatttcgtg aaaaccacgt cgatgtagct ttttcgttca
      721 cagtgctcga agagggaaat gccgatcgca tgctcgccga attggtcagg gtcacgaagc
      781 ccggtggacg gattgcggtg atggtgcgcg ccttggatat tccgtgggtg atcaatgtgc
      841 cgctacgccc tgagttaaaa acgaaagtgg aaatcccgca cggattcgtt gccgcagagg
      901 gttgtgccga tggcggtctc taccgacgtt tacacgcggc aggactgaca caagtacaga
      961 tgttcccgca gtttgccgcc tttaatcgtc ccgataccgc gcaagcgcag tttgcccaca
     1021 gcgcgattgc cagtagcctt aacccggagg aaacgcagga gtggcgcgcg gcggtcgccc
     1081 acgcggtcgc ggacggaacc tattttattg cgcagccgtt tcactgtgct ctcggaacca
     1141 agcccgaact gtgagatagg cgaccggtca gaccccgcaa tctcgcagaa tggaatcttg
     1201 cgcgtctcga tcagcataaa cgttgaagcc atagcgctgg cggtattctt ctacccgctc
     1261 cagataacaa cactatgcac atcgccgcgc aagattactt ggcgctgatt gccacggcaa
     1321 tcacatcgta gaatgcttcg ggattactgc accgcggttt tgcgggcctc tgccccgcga
     1381 cccgtaaata agtaaggcgc aaacctgatc ctaagcagat ggcggccccc tacctcccgc
     1441 cgttcatcta ctgcgctcgg cccactcctt ggccttggcg atcgcgatgc ggatcgccgt
     1501 accttcatcg taaccctctt cgagtaaggc attggcgatt tcgatcgcct tcaaccgcac
     1561 ttcgcgcgac aaattgcgca tcgactttgg gtagtaaact tcgttccacg gcatcgattg
     1621 gttcccaact ctctttcatt ccagccagag acaacctctg gatcacgata gcatttcacc
     1681 agacaacgct tgcgatcgga atcgggagcc ttgagcgaca aagatcaaac tcccaaacgc
     1741 gatcgattcc gacgacctcc atcctgcaaa gtcgcagggt gcccaacgct tgctcgattc
     1801 aagatcggtg tgctctttga aatcacctac gttcgcaaaa tcacacagca ttgctagcgc
     1861 ctcaagatcg acaattagta tggttccgag acgggtattg aatctcatgg tagccgctgt
     1921 gcccattctg caggataatt cacaatgcgg gcgcgcaccc tgttgagatc gatgtagacc
     1981 aaacagcgat gtagatgttc gtgcccttcg attgcggttg cgtgaacgat ctttccgaag
     2041 gtcccacgtc gatcctaacg ataattctac tcgtgtgctg tacggccggc attcaactgt
     2101 atggttcgcg cgatgccatt ccccgccgtg tccttcgcca gaagatagac gtgattggag
     2161 gtcacgattt ttcgaaagtc tctaaagata ctagaacggc cgtcctcgtg ctgtgccacg
     2221 ctgctacatt cttcaacatg acgccctcgg tccgggtctt ctgacgtgag tcgccttgca
     2281 tctgtccgca cggacagaaa taatgaggta ttcagaagtt gaacttacag tgctcacctg
     2341 atggaagttg tcggcggctc gaattagaca gaggatcgaa cgaaaccgaa tgaatagcga
     2401 ccctatcctc cggtttaaag gattgttagc cgaagcggcg caactgggta ttgtgccgta
     2461 taacgcggcg gctttcgcca cgacggatag agacctgcaa ccgaccgtgc gcatgctgct
     2521 gctaaaagat gtcgatcact gcggccttgt cttttacacg aacctggaga gccgcaaggc
     2581 gcggcagctg agcgataacc cgcgggcatc ggcctgtttc tggtggccgc aactgcggca
     2641 gcaagttcgc ttcgagggcg cggtcgagct cgtcagcgag cgagaagccg acgattattt
     2701 cgccagccgt ccacgcggca gccagatcgg cgcctgggca tcgcaccaga gcagcgagct
     2761 ctcatcgcgt gacgagctca tggccgcggc tgcctccttc agcgccaagt acaaggaccg
     2821 gacggtaccg cgtccgccgc actggtcggg ttaccgaatg ttcgccgaac gcattgagtt
     2881 ctggcgagaa cagcctgacc gtttacatga ccgcgaactt tacacgcgcc aagccgacgg
     2941 ttggaatatc atgctgctag cgccgtgaag aacgctgctt ctatccttct atctaggaaa
     3001 aagaaagcta gacagcctgc ctagtccgat gggttgtgga agaccgtagc acgaccgctg
     3061 ccgcgattag tgagagggta tgcgtcccct cctgtcgtcc tcggacgaa
//
LOCUS       DASUTG010000099         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_312361, whole genome shotgun sequence.
ACCESSION   DASUTG010000099 DASUTG010000000
VERSION     DASUTG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312361"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VF089_02205"
     CDS             complement(<1..177)
                     /locus_tag="VF089_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6172799.1"
                     /translation="MNGCLEGIRVLELGNFISGPYGAMLLADLGAEVIKIENPQGGDP
                     FRGWDLGGDQPNFWA"
     gene            complement(378..1553)
                     /locus_tag="VF089_02210"
     CDS             complement(378..1553)
                     /locus_tag="VF089_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6172800.1"
                     /translation="MKGSLEGIRVLELGNFISGPYGAMLLADMGAEVIKVENPKGGDP
                     FRGWDFGGDQPNFWAYNRGKKSVTLNLQTAEGKEIFYGLVKKADVVMENYRPGVTKKL
                     AIDYDTLRKINERIICCSISGTGPTGPYVNRPAYDTVGQGLGGMMSVLMDPKDPKPIG
                     PAYADCLSGMFSALGVLGALVSRSQTGKGQQVDATMAGSILAFLIAPATETLAHGQPP
                     GPYTRPRQSQTYAFSGSDGLPFAIHLSSPQKFWEGLCKSVDRPDLIEDARFKTRPDRR
                     KNYDDLHKTFAAIFKEKPRSHWLERLEANDVPHTPVYNLKEVFEDPQIKHMGLEITIE
                     RATRPTIRTVKFPVEYSDTKIPHPAPPPELGEHNNEFLRSLGYDEKRIAELKEKAVI"
     gene            1662..>2430
                     /locus_tag="VF089_02215"
     CDS             1662..>2430
                     /locus_tag="VF089_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6172801.1"
                     /translation="MATPPPVTDTLARFVAETDYSAISENALSQAKMHLLDTIGAGLA
                     AVSTPVAAIAFDYCKRVGDSQQASIWGTNEKASAPMAAFTNGLLAHALDFDDWDAYLR
                     AGHPSSVITAAALAIGESIGASGKDLLKAYVFGIEVLTQLAAGTPELHKRGFHSTPVW
                     GSIGAVIASASLLKLDPEKIKAALGVATSGAAGIQRQQGSMVKPFHAGNAARNGVEAA
                     LLAQQGFTADQNIIEAPRGFCDTFFGPATCDYEKMLRG"
BASE COUNT          500 a          686 c          672 g          572 t
ORIGIN      
        1 agcccagaaa ttcggttgat cgccacccag atcccaaccg cgaaatggat cgccgccttg
       61 cgggttttca atcttgatga cttctgcccc cagatccgca agcaacatcg cgccataggg
      121 gccggaaata aagttgccca actccaaaac tctgatgcct tctaaacaac cattcatact
      181 acttctcctt gtgattgcgg ctaagctcta ggacattccg tgattgatct cgtgaggcac
      241 tttgcgtcac ccttttttct ctcccgctta gcaaagggga gatttagtag gggttctgaa
      301 tacggggcct tggcgccacg aagaactatg aagtgctacc gcttcgatcg agtttctatt
      361 caatgcgctt agctcaatca gatcacggct ttctctttga gctcggcgat ccttttttcg
      421 tcatagccca acgatctcaa aaattcattg ttgtgctcgc cgagttcggg cggcggcgcg
      481 ggatgcggaa ttttcgtgtc gctatattcc accgggaact tcactgtgcg gatcgtcggt
      541 ctagtggcgc gctcaattgt tatttccaga cccatatgct tgatctgcgg atcttcaaaa
      601 acttccttca gattataaac cggcgtatgc ggcacatcgt tggcttcgag gcgttctaac
      661 cagtgcgagc gcggcttctc tttgaagatc gctgcgaagg ttttgtggag gtcgtcgtaa
      721 ttcttgcgcc ggtcagggcg ggttttgaaa cgcgcatcct cgatcaagtc gggccggtcc
      781 accgacttgc agagcccctc ccagaatttc tgcggtgacg acaaatgaat tgcaaaaggc
      841 aatccgtcgg aaccggagaa agcataggtc tgagattggc gcggccgggt gtagggaccc
      901 ggaggttggc cgtgcgctag ggtctctgtg gccggggcga tcaagaacgc gaggatggag
      961 ccggccatgg tcgcatccac ctgctgtcct ttgccggttt gcgatctcga caccaatgcg
     1021 ccgagcacgc cgagtgcgga gaacatgccg ctcaagcaat ctgcgtaagc gggaccaatc
     1081 ggcttgggat cttttgggtc cattagcacg ctcatcatcc cgcccaatcc ctgccctacc
     1141 gtatcgtagg ccggccggtt aacgtaaggt cccgtgggcc cggtgccgct gatggagcag
     1201 caaatgatcc gctcgttgat tttgcgcaat gtgtcataat cgatggctaa ctttttggtg
     1261 acgccggggc ggtagttttc catcaccacg tcggctttct tgaccaggcc ataaaagatt
     1321 tccttgcctt cggctgtttg caggttcaag gtcacgcttt ttttgccgcg gttgtaggcc
     1381 cagaaattgg gctggtcgcc gccaaaatcc cagccgcgaa atggatcgcc gcccttggga
     1441 ttctcaactt tgatgacttc ggcgcccata tctgcgagca acatcgcgcc atagggtccg
     1501 gaaataaaat tgccaagctc caaaactctg atgccttcca aactgccttt catgcgtgta
     1561 cctcttttat ctttgtccgg cgccgtcggc ggcgtccaat agacagtggc gatcgattta
     1621 gatattggac tttcatacct tccaattcgc aggagaacaa gatggcaacg ccgccgcccg
     1681 tcacggatac tttagctcgc ttcgtcgctg agaccgacta ttcggccatt tccgagaatg
     1741 ccttgagcca ggcgaaaatg cacctgctcg ataccattgg cgccggattg gcggcggtga
     1801 gcacgccggt ggccgccatt gcgttcgatt actgcaagcg agtgggagac tcacagcagg
     1861 cttcgatctg ggggacgaac gagaaagctt cggctccgat ggcggcgttc accaacggtt
     1921 tactagcaca cgctttggat ttcgatgact gggatgccta cctccgcgcc ggtcacccga
     1981 gcagcgtgat cactgccgcc gcgctcgcga ttggcgagag tatcggcgca tcgggaaagg
     2041 atttgttgaa agcctatgtt tttggaatcg aagtcctcac ccagctggcc gcgggcactc
     2101 ccgaattgca caaacgcggt ttccacagca cgccggtgtg gggcagcatc ggcgcggtca
     2161 tcgcatcggc cagtttgctc aaactggatc cggaaaaaat caaagccgct cttggcgtgg
     2221 caacctcggg cgctgcgggg atccagcgtc aacagggctc gatggtcaaa ccgtttcatg
     2281 ccggcaatgc cgcccgcaac ggcgtcgaag ccgcgcttct cgcgcagcaa ggttttaccg
     2341 ccgatcagaa tatcatcgag gcgccgcgcg gcttttgcga cacattcttc ggaccggcaa
     2401 cttgcgatta cgaaaagatg ctgagaggca
//
LOCUS       DASUTG010000100         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_312433, whole genome shotgun sequence.
ACCESSION   DASUTG010000100 DASUTG010000000
VERSION     DASUTG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312433"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF089_02220"
     CDS             <1..218
                     /locus_tag="VF089_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172802.1"
                     /translation="TGDISGASCIVDATEPLPGRSDVAYFPPCLNRRTIFLVPYPLVP
                     ANNLRAECSGRLSLQLMVASRFRSESG"
     gene            610..2898
                     /gene="priA"
                     /locus_tag="VF089_02225"
     CDS             610..2898
                     /gene="priA"
                     /locus_tag="VF089_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX6172803.1"
                     /translation="MSHELAKFARIIIPSPVKEPLTYGVPDNLRHLVTIGMRVLIPLG
                     KRKVSGIVFELIPETPLHHIKDIIAVLDEEPIVDEPLLTLSQWASQYYVSSLGEVLST
                     MLPPTSRTEIESIVVVRSESMQTDHVLGQKILDVLREKNGRTSLKRLTRALGGANISR
                     AIKQLQSLGHVEIRERLPGQKRRPRFDGAKSSPSPSADLRRFTLSTQQHAALAAMKTG
                     LKTGGFATFLLHGVTGSGKTEVYLRAMECVRDTGRQSLILIPEISLTPQLLDRVNARF
                     NGRVGVLHSGLTAAERWAQWWQIKRGDVDIVVGARSAVFAPVPSLGLIIADEEHDGSY
                     KQDDGLRYNARDVAVMRGKLCACPVLLGSATPSLESYENCRQGRYHLLEISERVEQRP
                     LPSVEIVDLRNHYNSTGMTVTHAGADAWDKQKSAGERLLSYPLATAIKRNFEDNRQTL
                     IFLNRRGFANFLQCTRCGYVLRCSYCSVTLTLHLKHKVLRCHHCDFSKPVQRICPECR
                     NETLAGVGAGTEQIEKTLHELVPEARIARMDRDTTGKRGSHADLIRRWESGDIDILIG
                     TQMITKGHDVSGVTLVGALLADMSLNLPDFRSAERTFQILSQVAGRCGRGNDPGRVII
                     QTYGPDHYAIQPLINHDYKNFFAQEIEFRRALGYPPFGKLVNLRLDGPNPEHVEAKAR
                     TVFKNLRAIQSGNPKYREHIEILGPAPSPIEKLRNRYRWQLLLKGNQIGPLIGLASAV
                     RDTFTKARNVRLHIDVDPYNML"
     gene            2911..3450
                     /gene="def"
                     /locus_tag="VF089_02230"
     CDS             2911..3450
                     /gene="def"
                     /locus_tag="VF089_02230"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX6172804.1"
                     /translation="MALLEILKYPDPRLSKAAHPVANVTDTTVQLISDMLETMYAAPG
                     VGLAATQVGVLQRIVVMDVDHENPRTTIYKLINPVITHSEGQLVWEEGCLSVVDFTAE
                     VRRAEHVQVVALDERGKERKIEAEGLLAVALQHEIDHLDGKLFIDRISRLKRDLYTRR
                     RKKMLRSGIEQSESAQIMI"
     gene            3505..>3998
                     /locus_tag="VF089_02235"
     CDS             3505..>3998
                     /locus_tag="VF089_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6172805.1"
                     /translation="MSSSWPIVFMGTPEIAAVTLQILSDGSDPVVGVVTQPDRPTGRG
                     QQSRASPVRRMAERRGIPVITPMKIRDSGFVEALRSWNPQLMVVVAFGRILPKSILDL
                     SPRGCLNVHYSLLPKYRGAAPVTWTIINGEEKGGVTTMQLVEQLDAGPIYLQEEILLD
                     EHETT"
BASE COUNT          991 a         1027 c         1056 g          924 t
ORIGIN      
        1 gaacaggcga catttccggg gcctcgtgca tagttgatgc tactgagccg cttcccggac
       61 gaagcgatgt ggcttatttc ccgccctgtt tgaatcgccg aacgatattt ttagttccct
      121 atccgctggt gccagccaac aacttgcgcg ccgagtgctc ggggcgcttg agtttacaac
      181 tgatggtagc gtcacggttt agaagtgaga gcggctaacg ctaaaattat gccaccctgg
      241 gtaaagggtg acatttccga gacataccca tttattcggc gaatgctccg gtccttagca
      301 aggccataat gtgccgagtt ggacaattat ggacaggatt acgtagagta cgtcagttat
      361 gggtgaaaac cgtaagagat cctttgggcg cgccaatcag ccaggtcagt aacctgaagc
      421 aagtaccgat acccactgac gtgcctaggc tcttctggag atatagtccg tgaccaatgt
      481 gtgtattggt caccgggtgt ttcgggcggc tcgaacagca cagtaacggc tatttaaatt
      541 tttgatcctc attgaattta cgagtatcgc aagttgccat ttgaactgtc ggaagttatt
      601 tatccagcga tgagccacga actggcgaag ttcgcccgca tcatcattcc gtcgcctgtg
      661 aaagagcctc tcacctatgg tgttcccgat aacctaagac atcttgtgac cattgggatg
      721 cgcgtgttga ttccactcgg taaacgcaag gtctcgggga tcgtgttcga gcttattccc
      781 gagacgccgc tgcaccacat caaagatata attgctgtct tggatgaaga gccaattgtt
      841 gatgaaccgt tattgaccct cagtcagtgg gcgagccaat attatgtatc gtctcttggt
      901 gaagtactga gcacgatgct accaccaact tcccgtacgg aaatcgagag catcgtcgtc
      961 gtcagatcgg aatcgatgca gactgatcat gttctaggac agaaaatcct agacgtgctc
     1021 cgcgagaaaa atggccgaac cagccttaag cgcttgacga gagctcttgg cggagcaaat
     1081 atttccaggg cgatcaaaca acttcagtcc ctcggccacg ttgaaatccg cgaacgttta
     1141 cccggccaga aacgaagacc gcggttcgac ggcgctaaat cctccccatc accgtctgct
     1201 gacctaaggc gcttcacgct cagcacgcaa caacatgcag cactggcggc gatgaaaact
     1261 ggcctcaaga ccggcggatt cgcaaccttt ctcctgcacg gcgtgacggg cagtggtaaa
     1321 actgaggttt acttacgcgc catggaatgc gtgcgagaca ctggccgtca aagtcttatc
     1381 ctaattccag aaatatcatt aactcctcag cttctggatc gggtcaacgc aagatttaac
     1441 ggcagggtcg gtgttttaca cagtggtttg acggccgcgg agcgatgggc gcagtggtgg
     1501 cagatcaaac gaggagatgt cgatattgtt gttggggcgc gctccgcggt ttttgcgccg
     1561 gttccaagtc tcgggttgat tattgccgat gaagagcatg acggatccta taagcaggat
     1621 gacggcttgc gctacaacgc cagggatgtt gctgtcatgc gcggcaagct ctgcgcttgt
     1681 cccgtacttc tcggctcggc gacaccatcg ttggagagct atgaaaattg ccgtcaaggt
     1741 cgttatcatt tgctggagat ttccgaacgc gtagagcagc ggccattgcc tagtgtcgaa
     1801 attgtagatc tccggaacca ttacaactcc accggtatga ccgtcaccca tgctggggcg
     1861 gatgcatggg ataaacagaa gagtgctgga gaacgattgt tgtcatatcc gctcgcaacg
     1921 gcaatcaaac gaaatttcga agataaccgc cagacgttga tctttttgaa tcgccgaggg
     1981 ttcgcaaact tcttacagtg cacgcgatgc ggttacgtct tgcggtgctc ttattgcagc
     2041 gtcacattga ccttgcattt aaaacacaaa gtacttcgct gccatcactg cgatttcagc
     2101 aaaccagtac agcgaatttg ccccgaatgc cgcaatgaaa cccttgcggg tgttggggca
     2161 ggaactgaac agatcgaaaa aaccttacat gaacttgtcc ctgaagcgcg catcgcacgt
     2221 atggacaggg acaccaccgg gaaacgcggc tcgcacgcgg acttgatccg aaggtgggaa
     2281 agcggcgata tcgacatact tatcgggacc caaatgatca ccaagggaca cgatgtttcc
     2341 ggagtgacgc tagtcggagc gctgcttgcg gatatgtctt tgaatctgcc ggattttcgg
     2401 agcgctgagc gaacctttca aatcctcagc caagttgcgg gaagatgcgg acgaggcaat
     2461 gatcccggac gagtgatcat tcagacctat ggacctgacc attacgcaat tcaaccgctc
     2521 attaaccatg actacaaaaa cttttttgcc caggaaatcg agtttcgacg ggcgttgggc
     2581 tatcctcctt tcggcaaatt ggtaaatttg cgcctagacg gtccaaaccc ggagcatgtg
     2641 gaagcgaaag cccgcacagt tttcaaaaat ctccgcgcga tccaaagcgg caaccccaag
     2701 taccgcgagc acattgagat tctcggtccc gctccgtcac cgattgagaa actacggaat
     2761 cgctatcgat ggcaactctt gctcaaaggc aaccaaatag gccccttgat cgggttagcc
     2821 agtgccgttc gcgacacatt cacgaaagcg cgcaacgtgc gtctgcacat tgatgtcgat
     2881 ccctacaata tgttatgaaa ataatagctt atggctcttt tagaaatcct gaaataccct
     2941 gatccacgac tttcaaaagc ggcacacccg gtagcgaatg taaccgatac taccgttcaa
     3001 ctgatcagtg acatgctcga aaccatgtat gccgcccccg gagttggttt agctgcgacg
     3061 caggttggag tgctgcagcg gatcgttgtt atggatgtgg atcacgaaaa tccgcgaact
     3121 accatctaca agctgatcaa tcctgttatc actcactccg aggggcaact cgtctgggag
     3181 gaaggatgct tgagtgtggt cgattttacg gctgaagtac gccgcgcgga acatgtgcaa
     3241 gttgtggcgc tagatgaacg cggaaaagaa cggaaaatag aggcggaggg tttgctcgcc
     3301 gtagcacttc aacatgagat cgatcatttg gatggaaaac tgttcatcga tcgtattagt
     3361 cggttaaaac gcgacctcta cacacgtcgt cggaagaaga tgttgcgcag cgggatcgag
     3421 caatccgaat cggcgcagat catgatctag ctgcttcgag aagtcgcagc tgaagattca
     3481 atcaaagtct ctcaggcgat ctccatgtct tcctcttggc ccatcgtatt tatgggaact
     3541 ccggagattg ccgccgtcac attgcaaatc ctgagcgatg gctccgatcc cgttgtcggg
     3601 gtggtcacac agccggaccg tcccacaggg agaggacagc aaagcagggc ctctccggtc
     3661 cgccgcatgg cggagcgccg ggggattcca gtgatcacac ccatgaagat ccgcgattcg
     3721 ggttttgtcg aggccctgcg atcatggaat ccgcagctta tggttgtcgt agcgttcgga
     3781 cgcattctac ccaagtcgat tttggatctg tccccacggg gatgtttaaa cgtgcactac
     3841 tctctcctgc ctaaatatcg cggtgcagcg cctgtcactt ggacgatcat caatggagaa
     3901 gaaaaaggcg gcgtcaccac gatgcagctg gttgaacaac tagatgccgg accgatctac
     3961 cttcaagaag aaattctact cgatgagcat gaaacaac
//
LOCUS       DASUTG010000101         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1350403, whole genome shotgun sequence.
ACCESSION   DASUTG010000101 DASUTG010000000
VERSION     DASUTG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350403"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..364)
                     /locus_tag="VF089_02240"
     CDS             complement(29..364)
                     /locus_tag="VF089_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172806.1"
                     /translation="MSVRTNLCPQRFGVGAVPPDASAALTAYLRLFGSPFLGIAVLNR
                     LTRNAEPSTARKAIIIGNIVGFAARAALDLWGLFSGARPLTKVFAMIHLLFAVDFIWV
                     GGINMSGSK"
     gene            complement(674..1072)
                     /locus_tag="VF089_02245"
     CDS             complement(674..1072)
                     /locus_tag="VF089_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX6172807.1"
                     /translation="MSESSSIETEAARRVAEAVPGIPRDDNGPVFHAPWEAHAFAMAL
                     ALYQKGLFAWSEWSAMLGEEIKKAQAAGDPDTGETYYLHWLATLERMVAEKGATSPQA
                     LAQHYQAWERATHRTAHGKPIELKPEDFDH"
     gene            complement(1065..1724)
                     /gene="nthB"
                     /locus_tag="VF089_02250"
     CDS             complement(1065..1724)
                     /gene="nthB"
                     /locus_tag="VF089_02250"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX6172808.1"
                     /translation="MDGVHDMGGMDGFGKVELEPNEPVFHHPWEGRVLAMSRAIGIFR
                     AWTIDTSRYAIELMAPSIYLTRSYYEKWFLRNQNLLIERGLIDADEVAAGRALRPAKN
                     LTRGPFTLADVEKVTKRGSYSRPAPAPAQFKVGDRVRTKNIHPKTHTRLPRYARGRVG
                     VVERLHGAHIFPDSVTIGKGEDPQWLYTVRFDAQELWGADTDPKLKVSIEAFEPYLEH
                     V"
     gene            complement(1724..2350)
                     /gene="nthA"
                     /locus_tag="VF089_02255"
     CDS             complement(1724..2350)
                     /gene="nthA"
                     /locus_tag="VF089_02255"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6172809.1"
                     /translation="MAKHDHDHSEGSDLSEMELRVRALETILVEKGYIESAALDRIVE
                     LYETKIGPHIGAQVIAKAWSDPDFHRALFEDASKAVSGLAHQVGNVGEHLIAVENTPQ
                     VHNMIVCTLCSCYPTDVLGLPPAWYKSFAYRSRAVKEPRAVLADFGVTLPVETEIRVW
                     DSTAETRYLVVPMRPAGTEGWSEAKLAKLVTRDSMIGAGLAKKPSEVA"
     gene            complement(2559..3035)
                     /locus_tag="VF089_02260"
     CDS             complement(2559..3035)
                     /locus_tag="VF089_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172810.1"
                     /translation="MADDILIDCGGAYANAAVRSVLENYLPSDVLSAFDGRLAFVSTA
                     RVDGVRLTKNFCRDRDVIVLSERIIPVTSEDEEFHPGYRYFIFVVLREIAHACKEHVS
                     PFSNDLTAPQVESQTREAEELALKWFNEHASTTLFQPPITITEIEELRGKARASPE"
     gene            3289..4149
                     /locus_tag="VF089_02265"
     CDS             3289..4149
                     /locus_tag="VF089_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6172811.1"
                     /translation="MTQKPFTWPNKARVAFVLGIAFEAWDRTKPTTGRGLQRGSIPAN
                     AVCRRDYSTETFREFGAKAGLRRLLDICDQYDLKVSMPINGLTCEYYPELVKEAHQRG
                     HEMIAHGWDQGEYLYMLTREQERENIFKTVEAITRVTGERPSGWSSPGVRSTDNTLEL
                     NVEAGLLYNCDYHDDEMPYPRRIGDKLLMMIPHQYTINDHRMEEEGTRHEFFEKFKDE
                     FDYRYKIGVDNPTMMNVISHAYLSARIPVIDTFEQIIAYAKRHKDVWFARRGDIAAWA
                     RKYFEENESR"
BASE COUNT          959 a         1164 c         1167 g          904 t
ORIGIN      
        1 cacttagtca gcgacgtcct tatttcttct acttactgcc tgacatgttg attcccccaa
       61 cccagatgaa atccacggcg aaaagcagat gaatcatcgc gaacaccttt gttaacggtc
      121 gcgcgccgct aaacaatccc cacaaatcca aagcggctct agcagcgaat ccgacaatgt
      181 tgccgatgat gatggccttg cgcgccgtgg acggctccgc gttccgcgtc aacctgttaa
      241 gcactgcgat ccctagaaag gggctgccga aaagccgcag ataagcagtc agcgcggcag
      301 aggcgtcagg gggaacggcg ccaacgccga agcgctgtgg gcaaagatta gtccgaaccg
      361 acaagagcca tgtagatcga ggcgatactg agcagaatcc tgagagtcat gggaaagctt
      421 cccttcggta attgagttct gaatacgcac aagcacagga gggatttccg gtagttggta
      481 gaggcgagag ttgagcgttg ctaactagcg ggaccaacta ctgcttccta ggtcggaaat
      541 ttactgtcca gctatttgtt ctcttcaagg cgaatgttag cagcacttgc aagaacatca
      601 ggtgcggtct ggtggtaaat cacgggagaa gatccggaac atttctttcg cgacagcagg
      661 taggcgccga gcctcagtga tcaaagtctt ccggcttcag ctcaatcggt tttccatgcg
      721 cggttcggtg ggtcgcgcgc tcccaggctt ggtaatgctg cgccagagct tggggcgagg
      781 ttgcgccctt ttcggcgacc atgcgttcga gcgttgcgag ccaatgaaga tagtaggttt
      841 cgccggtgtc gggatcgccg gcagcctgcg cctttttgat ctcctcgcca agcatcgcgg
      901 accactcgct ccaggcgaac aaaccttttt gatagagcgc cagagccatg gcaaacgcgt
      961 gggcttccca cggcgcgtga aacaccgggc cgttatcgtc gcgaggtatg cctggaacgg
     1021 cttcggcgac gcgccgggcg gcttccgttt cgatggacga tgattcagac atgttcgaga
     1081 taaggttcaa aggcttcgat ggagactttc aatttcggat ctgtgtccgc gccccaaagc
     1141 tcttgggcgt cgaaacgcac cgtgtaaagc cactgcggat cctcgccctt gccgatcgtc
     1201 accgagtccg gaaagatgtg cgccccatgc aggcgctcca cgacaccgac acggccgcgc
     1261 gcatagcgcg gcaaacgcgt gtgcgtcttt gggtggatgt ttttcgtgcg cactcggtcg
     1321 ccgactttga actgggcggg tgctggcgcc ggccggctgt acgaaccacg ttttgtgact
     1381 ttttccacat cggctagcgt gaacgggcca cgagttagat ttttagccgg acgcagcgcg
     1441 cgcccagcgg ctacttcgtc ggcatcgatg aggccgcgct cgatgagcag gttctgatta
     1501 cggagaaacc atttctcata ataggagcgg gtcaaataga tcgacggcgc catcaattca
     1561 atagcataac ggctggtatc gatcgtccag gcgcggaaga tgccgatggc acggctcatc
     1621 gccagcacgc gcccctccca cggatggtga aataccggct cgttcggctc gagctcgact
     1681 ttgccaaacc cgtccatacc gcccatatcg tggacgccat ccatcaggcc acctcgcttg
     1741 gttttttcgc cagaccggcg ccgatcatgc tgtcgcgggt gaccagtttg gccagctttg
     1801 cttcgctcca gccctccgtt cctgccggcc gcatcggcac gacgaggtag cgggtctcgg
     1861 cggtcgagtc ccaaactcgt atctcagttt cgaccggcag cgtgacgcca aaatccgcca
     1921 gcaccgcgcg cggctccttc acggcgcgcg agcggtaggc aaacgatttg taccaggccg
     1981 gcggcagccc gagcacatcc gtcgggtaac aggaacacaa cgtgcacacg atcatgttgt
     2041 gcacctgcgg cgtattctcg acggcgatca gatgctcgcc gacgtttcct acttggtgcg
     2101 caagcccaga gactgccttg cttgcatcct cgaagagggc ccggtgaaaa tcaggatcgc
     2161 tccacgcctt ggcgatcacc tgcgcgccga tatgcgggcc gatcttggtt tcataaagct
     2221 cgacgatgcg atcgagcgcg gcgctttcga tatacccctt ttcgacgagg atcgtctcga
     2281 gcgcccgcac ccgcagctcc atctcggaaa gatcggagcc ttcactatga tcgtgatcat
     2341 gtttagccat aggcaaaata tagccaagga aggtggcgtt gtgcaatcga ttgaatgaac
     2401 ttgacccttg aggaggggaa tccggttcag tcatcggtta tttgggttaa gcgtttgaac
     2461 tcgttgaaca gcttcttgag tattggaatt atccaagaat gatcagaatt aaatgacttc
     2521 gattgtaacg caacatcaaa ggtgaccttt gttttaactt attcggggct cgctctggcc
     2581 ttgcccctaa gctcctcaat ctcagtaatg gttataggcg gctggaacag agtcgtcgag
     2641 gcgtgctcgt tgaaccactt caacgcaagc tcctcagcct cgcgggtctg actctctacc
     2701 tgcggcgccg ttaaatcatt cgaaaagggc gagacgtgct ccttgcacgc gtgcgcgatc
     2761 tcacgaagaa ccacgaagat gaagtaacgg tagccggggt gaaactcctc gtcttcggat
     2821 gtcactggta tgatacgctc cgacagcacg atgacatcac ggtccctgca gaagttcttg
     2881 gttaaacgaa ccccatccac acgcgcggta gagacgaaag ccagccggcc atcaaacgcg
     2941 ctgagcacgt cagacggcag gtaattctcc agcacagaac gaacggcggc atttgcatac
     3001 gctccgccac aatcaatcaa gatatcgtca gccataactt cggcttgtag acgaacatgc
     3061 aggagtggtc aagtgagaat gcagatacgg tttgcctgat tgttgaggat ttcggtttca
     3121 ggccactgtg cactgcgtcg ccgttttcaa ccaagatgcc catgcgggat caatctcaag
     3181 agaaatcgtc agataacctg aaaatccgac ttccgccttg ctgagttaga aaactttagc
     3241 tataagccct gcttgtccga tgacttctat tcagtcggag gatcttacat gacgcagaag
     3301 cccttcacat ggccgaacaa agcccgggtc gcctttgtcc tcggcatcgc atttgaagct
     3361 tgggaccgaa ccaagcctac cacaggtcga ggattgcagc gcggctcgat acccgctaat
     3421 gcggtctgca ggcgcgatta ttccacggaa acatttcgtg agttcggcgc caaagcaggt
     3481 ttgaggcggc tgctggatat ctgcgatcaa tacgatctca aagtcagtat gccgatcaac
     3541 ggattgacct gtgagtacta tccggagctg gtcaaggagg cccaccagcg cgggcatgag
     3601 atgattgccc atggctggga tcagggcgag tacctctaca tgctcacccg cgagcaggag
     3661 cgagaaaaca ttttcaaaac ggtggaggca attaccaggg tgacgggcga acgaccatcc
     3721 ggttggtcgt ctcctggggt ccgttcaacc gacaacacgc tggagctcaa cgtcgaggcg
     3781 ggtcttttat acaattgcga ttatcacgat gacgaaatgc cttaccccag gaggatcggc
     3841 gacaaattgc tgatgatgat tccgcaccag tacacgatca acgaccaccg catggaggag
     3901 gaaggcacgc gacacgaatt ctttgaaaaa ttcaaagacg aattcgacta tcgttataag
     3961 atcggcgtgg acaatccgac gatgatgaac gtgatctctc acgcttactt gagcgcgcga
     4021 attcccgtca tcgatacctt cgaacagatc atcgcgtatg ccaagaggca taaagatgtc
     4081 tggtttgccc gccgcggcga tatcgccgca tgggcgagaa agtattttga agagaatgaa
     4141 tctcgctagc aggttgctga tgcttaaaaa cgactcagag cgcagccggt cctc
//
LOCUS       DASUTG010000102         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2091935, whole genome shotgun sequence.
ACCESSION   DASUTG010000102 DASUTG010000000
VERSION     DASUTG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091935"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1008
                     /locus_tag="VF089_02270"
     CDS             343..1008
                     /locus_tag="VF089_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX6172812.1"
                     /translation="MEARHVTHALEDYLKAIYQLSEEDRPVIAARLAAETGVSPSTIF
                     ATLRRLGKEGYVKINRRKEIHLTEDGKQVAENIVRRHFLTERFLTDLLGLDWVKAHQE
                     AHRLEHAISQEVEERLAKLLSHPTTCPHGNPIPGASSTPGPKTMPLSEAVDGQDVELD
                     YITEGGERDVRLLGYLQTHGLRPGAKVHIVDVAPSLGMMTLTVNGDEFSLGIEAAKKI
                     RVH"
     gene            complement(1117..1365)
                     /locus_tag="VF089_02275"
     CDS             complement(1117..1365)
                     /locus_tag="VF089_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172813.1"
                     /translation="MAQGKHKHQLRLQELIGAAKQMNIEVRTEKLLREVGYKPRSGRC
                     RLNGQEVILIDRDAPLSDQIELLSALLAEEERQPSSRT"
     gene            1560..2396
                     /locus_tag="VF089_02280"
     CDS             1560..2396
                     /locus_tag="VF089_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6172814.1"
                     /translation="MIKNILLPLDGSEHSHAAFDYALWMTEKFDGTLFGQHVIDTISI
                     EGTFFHDISGSMGFEPYLDFSTKMREALEERGKLILEEFTERCRKSGIKHQTVLDMGL
                     IANEICERSKVADLVVIGHRGINEDFTTGLLGTTAESITRKCPRPVFVSTKKFKSIER
                     PLLAYDGSQRACAAMESAAEFCTVLGLPLSVLTIAKEDQLAVGILHQAKSYLDSYAID
                     VHYERARGYPEQKINDHLVNFNHDLLFIGAYGHRRIIEMVLGSTTEFVLRNSSCPVFL
                     TR"
     gene            2431..>2878
                     /locus_tag="VF089_02285"
     CDS             2431..>2878
                     /locus_tag="VF089_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6172815.1"
                     /translation="MKFTAAAIQMLASDDKAANLAEAARWVRQSAAAGAKLVALPEVF
                     IWRGAKRSERAAAEPIPGPASTAMADLARELGIYLLAGSILEEIPGSAKAFNTSLLFD
                     PSGKLIASYRKIHLFDVDLANNVSARESDTRAAGETVVIAETELCKM"
BASE COUNT          692 a          742 c          767 g          677 t
ORIGIN      
        1 cgggccaagc agcgctagaa tgttgccgga cgcaacttgc aaatcgcagc tttttaccgc
       61 cacggtattt ctgtagcgtt tgctgatccc tttaacttcg atgatgacca tcaaattttc
      121 ccgtctgtgt taataaccca aactgctaaa caggtctagc acaatggact gaggacggac
      181 ctacaggtgc ggagcgcgtg aaatcctacg accgttgcct caccttgaac ttcaacaact
      241 cctgaaatag gatgtgatct gcgtgcaaca gcattttcag ctgcaacaaa caaccggcgc
      301 aacgccggtg gtcatatcct tgaaagagat ctaacagtca tcatggaagc tcgtcacgta
      361 acccatgccc tcgaagacta tttgaaagcc atttaccaac tttcagagga ggatcgcccc
      421 gtcattgccg ccagactcgc cgctgaaacc ggcgtgtctc cgtcgacgat ctttgccacc
      481 ctgcgtcggc tgggtaagga aggctatgtt aagatcaatc ggcgcaagga aattcacctg
      541 acggaagatg gtaagcaagt agcggaaaac atcgtccgcc gacattttct aactgagcga
      601 tttctcactg acctgcttgg tctcgactgg gttaaggcgc atcaagaggc gcatcgtcta
      661 gagcatgcca tctcacaaga agtagaggag cggctggcga agctgctgag ccacccgacg
      721 acttgcccgc acggcaatcc gatcccagga gcaagctcga cccccggacc gaagacaatg
      781 ccgctgagcg aagcggtcga tggacaggac gttgagctcg attatataac ggaaggtgga
      841 gagcgcgacg tgcgcttgct gggttacctc caaacgcatg gcttacgacc cggcgcgaaa
      901 gttcacatcg tcgatgtcgc accatcgttg ggcatgatga ctctgacggt caatggcgat
      961 gagttttctc tcggaattga ggcggcgaaa aaaattcgcg tacactgata ccggatcgag
     1021 tgccggccta acgttcaaca gcagaacagg cggattgcgc gcacatgatt ctcacgctcg
     1081 atgatcatga attgacgtac aatattcttg gggcgatcat gttcgagagg atggttgacg
     1141 ttcctcttca gcgagcagcg ctgacagaag ctcaatttga tcgctaagcg gcgcgtctct
     1201 atcgatcaag atcacttcct ggccattgag acgacaacga ccgctgcgcg gcttatatcc
     1261 tacttcccgc aataattttt cggtgcgcac ctcgatgttc atctgtttgg cggcgccgat
     1321 taattcctgc aatcgtaatt ggtgcttatg ttttccctgt gccatagctg aattaaaggt
     1381 aggttattat ctgctaaacc tgctgatttg taaaacgatt gaaccttttt cgggttgcaa
     1441 aaacggggca ggttgttatc gtagcccgcg gttgatcggc ggacgtcgct cggaatccgg
     1501 agccattgcg ctgattcagt gtacatcagg cttgccactt acggcttcag gagggaaatg
     1561 tgatcaagaa tatcttgctt cccctcgacg gttcggaaca ctcgcatgca gcctttgact
     1621 atgctctgtg gatgacggaa aaattcgacg gaacgctctt cggtcaacac gtcattgata
     1681 ctatctcgat tgaaggaaca ttttttcacg acatctccgg atccatgggc ttcgagccgt
     1741 acctggattt ctccacaaag atgcgcgaag ccctcgagga gcgcggcaag ctgatactcg
     1801 aagagtttac cgaacgttgc cggaagagcg gaatcaaaca tcagactgtt ttggatatgg
     1861 gcttgatcgc caacgagatc tgcgagcgct cgaaggttgc tgatctggtc gtcattgggc
     1921 atcgcgggat caatgaggat tttactaccg ggcttttggg gacgaccgcg gaaagcatca
     1981 cgcgcaaatg cccgcgcccg gtgtttgttt cgacaaaaaa gttcaagtct atagagagac
     2041 cgctgttagc ctacgacggc agccagcgcg cctgcgcggc gatggaatcg gcggccgagt
     2101 tctgtactgt attgggtcta cccctgagtg ttttgacgat cgccaaagaa gaccagcttg
     2161 ctgttggaat cttgcatcag gctaaatctt atttggactc gtatgcgatt gatgttcatt
     2221 atgaaagggc gcgcggctat ccggaacaga agatcaatga tcacttagtc aatttcaatc
     2281 atgatcttct atttatcggc gcttacggcc atcggcgcat catcgagatg gttcttggta
     2341 gcacgaccga attcgtactg agaaacagtt catgcccggt atttctaaca cgttgacaat
     2401 tgggtcagaa aatctgatcg aggaaactca atgaagttca ccgcggctgc aattcaaatg
     2461 cttgcctcgg atgacaaggc ggctaatctg gcggaagcgg cacgatgggt tcgccaatcc
     2521 gccgctgccg gcgccaaact tgtcgccctg cccgaagttt tcatttggcg tggtgcgaag
     2581 agaagcgaac gggcagccgc tgagcccata cccggacctg cctctacagc aatggcggac
     2641 cttgcgcggg agcttggcat ctacctgctg gcgggatcca tattggaaga aattcccggc
     2701 agcgcaaaag ctttcaacac gagcctgttg tttgatcctt ccggcaaatt aatcgcgagt
     2761 taccgaaaaa ttcacttgtt tgatgtggat ctggcgaaca acgtttccgc acgggagtcc
     2821 gatacccgcg ctgccggcga gacggtagtc atcgccgaaa cggagctctg caagatgg
//
LOCUS       DASUTG010000103         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_757324, whole genome shotgun sequence.
ACCESSION   DASUTG010000103 DASUTG010000000
VERSION     DASUTG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757324"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VF089_02290"
     CDS             complement(<1..726)
                     /locus_tag="VF089_02290"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6172816.1"
                     /translation="MIELDRMIIDKPGDGIFRIHRSAMTSLEVLQMEQERIFNKCWLY
                     LCHESEVEKPGDYRRRTVAGRPLFFIRGSDGRLRVFLNTCTHRGALICRHDHGHADVL
                     QCFYHAWSFNNHGDLVGVPDEAGYPDSFDRAKMGLKSPPRVDSYRGFVFVSFNADVEE
                     LVTYLAGAREYLDLIVDQAEKGMRIIPGSNRYSIKANWKLLVENSLDGYHVRPTHKTY
                     FEYVNSLGINTSGVQTAFSRPAVV"
     gene            complement(1065..2042)
                     /locus_tag="VF089_02295"
     CDS             complement(1065..2042)
                     /locus_tag="VF089_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6172817.1"
                     /translation="MSKLKLTLACWDYDRTRPLIDGRVQAEGIDLEVKVMRPREAFPR
                     MLEDQEFEVSELSLASYTSLLGRGTCPFVAVPVALSKIFRHSCIYIRADAGIRTPQDL
                     RGRRVGTTQLGSTATIFMNGMLQHEYGVTMEDIHWFIGGLTTPTQRPLIPLKLPDKVK
                     VEFLPADQTLEGMLESGDLDALLAIYIPSIFQQGSARIARLFPNYYEVEQDYYRRTGI
                     FPIMHTVVLRDDIYREHPWAARSIYQAFCEARDLAVEGLYDTDALRLSLPWLIHHIEE
                     TWKILGKDFWAYGLEPNWPTWKAIGEYVYEQGLSPRAVSPDELFAANVE"
     gene            2839..>3589
                     /locus_tag="VF089_02300"
     CDS             2839..>3589
                     /locus_tag="VF089_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172818.1"
                     /translation="MLHRLAMTAAIVAGMLASVTSSFAADDFYKGKTIKFLVGYRAGG
                     GYDTFTRAVARHIGRHIPGNPSASVENRGGAGSLIAANTIYNNTKADGLTAGVFGSGL
                     VTQQALDARGIRFDAQKFNWIGSMSEGTPVCAIMGFTGLDTLKDVLNSKKPLRMGSTG
                     SGSTTDDLPKLMNGLMGTNFDVISGFKGTSAVRLAMRRKELDGACWTWESMRATAKSM
                     LDATGDQRLIPFIIEGDYEDPEVTKLPEFTEV"
BASE COUNT          796 a          998 c         1021 g          774 t
ORIGIN      
        1 aacgacggcc ggccggctga atgcggtttg cacgccgcta gtgttgatcc caaggctgtt
       61 tacatattcg aagtaagtct tgtgcgtcgg cctgacatgg tatccatcca ggctgttctc
      121 gaccagcagc ttccagttgg cttttataga gtagcgattg gaacccggga tgatcctcat
      181 gcctttttcg gcctgatcga cgattaggtc gagatactcg cgagcccccg ctagataagt
      241 cacgagttct tcgacatcgg cattgaagct cacgaaaaca aagccacgat agctgtcgac
      301 ccgcggcggg gacttgaggc ccatcttcgc ccggtcgaaa ctgtcgggat agccggcctc
      361 gtcgggcacg ccgacgagat cgccgtgatt gttaaagctc caggcgtgat agaagcattg
      421 caaaacgtcg gcatgcccgt ggtcgtgacg gcaaatgagc gcgcctctgt gcgtgcacgt
      481 gttcaggaat acccgcagcc tgccgtcgct gccgcggata aagaaaagcg gcctcccagc
      541 tacagtccgg cgccggtagt cgcccggctt ctcgacttcg gattcgtggc agagatacag
      601 ccagcatttg ttgaatatcc gctcctgctc catctggaga acctcaagcg atgtcatggc
      661 ggagcgatgg atgcgaaata taccgtcccc cggcttatct atgatcatac gatcaagctc
      721 gatcatggcg tgcccctcat gtgttacatc gaccaggaac aatgtgaacc tttaggaact
      781 ataaagccgt aaaccggctg gaatgtgttc gccggcagag gtacgataca cgtctaccat
      841 gctagccggc aagattcacc gtccggtgaa acgccgagca gagtgtttgt ccgatgatta
      901 agcaactgtg agttccgctc cagtcgttcc ccctcccggg atcggggagg gggcaggcgc
      961 atgcatcctc ccccggcgtc gcgggggagg acaagaggtg ggggtttgaa cgatttgaac
     1021 gttttgaacc ctttggaacg actgcaacgc cgaagggcta gtaatcactc cacgttggct
     1081 gcaaacaact cgtcagggct gaccgctcgc ggcgataacc cctgttcgta aacatactca
     1141 ccgatcgcct tccatgtcgg ccaattcggc tccaggccat acgcccagaa atctttccct
     1201 aaaatcttcc aggtctcctc gatgtgatgg atcaaccagg gaagcgaaag gcgcagggcg
     1261 tcggtgtcgt agagtccctc gaccgccaga tcccgagctt cgcagaaggc ttgatagatg
     1321 ctccgggccg cccacgggtg ctctcgatag atatcgtcgc gtagcaccac ggtatgcatg
     1381 atcgggaaga tacccgtgcg acgataatag tcctgctcca cctcatagta gttcggaaac
     1441 aggcgcgcga tgcgggccga gccctgctgg aagatggagg ggatatagat tgcgagcagc
     1501 gcgtcgagat cgccgctctc cagcatcccc tccagggtct ggtccgccgg cagaaactcg
     1561 accttgactt tgtccggcag cttcaatggt atcaacggtc gctgggtcgg cgttgtcaat
     1621 ccgccgatga accagtggat gtcctccatc gtcacaccgt attcgtgttg cagcatgccg
     1681 ttcatgaaaa tggttgcggt cgaaccgagc tgcgtcgttc ccacccgtct cccccgaagg
     1741 tcctgcggcg tccggatccc cgcatcggcg cgtatgtaaa tacacgagtg tcgaaagatc
     1801 ttggacagcg ccaccggcac tgcaacaaag gggcaagtac cgcggcctaa cagactcgtg
     1861 taggaagcga gcgacagctc cgagacctcg aactcctggt cctccagcat tcggggaaag
     1921 gcctcgcgcg gacgcataac cttgacctcc agatcaatgc cttccgcttg gacacgacca
     1981 tcgatgagcg ggcgggtgcg atcatagtcc cagcacgcga gggtaagttt gagtttcgac
     2041 atcggtgcaa cctgtctcca gggaacttta gttgaacgtg gaataacaca ccgcgaggag
     2101 ctgtcaagaa atgcggttag tggccattgg tgtgacacgc catcagtgat cctttctctc
     2161 gcttgtcagg aactgctaga atctgtcatc tcaatgtgat ctatcgcata ttagcggact
     2221 atccgttgag aatttatcag atagacggaa tgatcgacgc gcacaagcgg cgtcgggtct
     2281 caaacatttt tgacggacgc cggggtatat gcggcgattg acgtcttgta tctgaataac
     2341 cgatggacga gttgtgttta cgcttccggc tttgaccgtt ttgaaccgag cgcagcgatt
     2401 gaccgatttg acatccttga acaaaatctc ttgtccgtca ggtgctagcg catccaggca
     2461 gaaaagtagc gggtcctggc ggcaagaatg cggtgaccaa cggccgtgag acttaggata
     2521 gtccgcccac agttcgaggc agatcgtgct cacgcgcagt gaacaatctg cacatgatca
     2581 aagtcactgc cgcccaaccc tgatcacctt cttaccacct cctggtgaac gtgctcgttt
     2641 cttgtttgat accgcggctg tggcgggtgc cggctaattc gaagtgcaac tggcatatcc
     2701 attgcgcttt ggatgactat tacagttcag ccgaatctca gcagatcgtt gttcgagttc
     2761 tgagccttct agcagctgat cgggatacta ttcgatccgc gggagagcgg cgcccaccta
     2821 aagaaaggag aatgccagat gttacaccga ttagcgatga ctgcggcgat tgttgctgga
     2881 atgctcgcat cggtcacgag cagctttgct gcagatgatt tctataaagg caaaaccatc
     2941 aaatttcttg tcggctatcg tgctggcggc ggctacgaca cgtttacccg agcggtagcg
     3001 cgtcacatcg gtcggcatat tccgggcaac ccgtcggcta gtgtggagaa ccggggcggt
     3061 gccggcagtc tgatcgcggc caacaccatt tataacaaca ccaaagccga cgggcttacc
     3121 gccggcgtgt ttggctccgg gctggtcacc cagcaggcgc tcgacgcgcg cggcatccgc
     3181 tttgacgctc agaaattcaa ctggataggc tccatgagcg agggcacccc ggtttgcgcg
     3241 attatgggat ttaccggcct cgatactttg aaggacgtgc tcaatagcaa aaagccgcta
     3301 agaatgggat cgacgggctc ggggtccaca accgacgatt tgccaaagct catgaatggg
     3361 cttatgggca cgaactttga cgtgatctcc ggctttaaag ggacgtcggc cgtgcgactg
     3421 gcgatgcgcc gcaaagagct cgatggtgcc tgctggacct gggaatcgat gcgcgccacg
     3481 gcgaaatcga tgcttgatgc cacgggcgat caaagactta ttccattcat catcgagggg
     3541 gattatgaag acccggaagt cacaaagctg cccgagttca ccgaagtca
//
LOCUS       DASUTG010000104         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2092125, whole genome shotgun sequence.
ACCESSION   DASUTG010000104 DASUTG010000000
VERSION     DASUTG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092125"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..652)
                     /locus_tag="VF089_02305"
     CDS             complement(329..652)
                     /locus_tag="VF089_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX6172819.1"
                     /translation="MPIYEYECQKCGTFETTQRITEEPLSKCPTCKGKVKKLISSTSF
                     QLKGTGWYITDYARKGKDAPKTENGASSSTSESKTESKESKTETKKESVPAKSESSST
                     STTPT"
     gene            complement(720..1115)
                     /locus_tag="VF089_02310"
     CDS             complement(720..1115)
                     /locus_tag="VF089_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6172820.1"
                     /translation="MDFVNLGEKQSEARDNKKRKIDLFGTDNFRSWMLYFVPGDGTDM
                     HYHASPETFLVLEGSGTVKGIKGDERPVKKHDVLFLGAKDYYQITNTGTEPLVLFGNR
                     SEAFGGAHILADGKDRNQVEQRTSESSGT"
     gene            1454..2161
                     /locus_tag="VF089_02315"
     CDS             1454..2161
                     /locus_tag="VF089_02315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6172821.1"
                     /translation="MTSSWESTNVDNSTMRLYLSVPERGGPVPGIVVVHGQSGLENFI
                     KEATHMLALQGYAAIAPDLYHRDGPDCKDDNMTKKSRVRDSNIIADITGAMQFLKSHR
                     QVDGARLGIVGFCMGGRIVYLMSAVSRELKAAVMFYGSSTMTPFGKGPSPFDRTREIS
                     CPLQGHFGEEDQNPSPEDMRKLDAELTRWNKPHEFHTYQGAAHAFANAGSANYRPHAA
                     ALAWPKATEFFSRHLIG"
     gene            2419..>3628
                     /locus_tag="VF089_02320"
     CDS             2419..>3628
                     /locus_tag="VF089_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6172822.1"
                     /translation="MTENSDGSESGRHHMHHYNAAAALIERNLALRPQKIAYIDDNGR
                     YTFAELAERVNRCAHAFCELGATLESRIMVCLSDTIDFPSVFLGAIKAGLVPVAVNTL
                     LTSGDYDFMLRDSRARILVLSESLLPVFKPILAGQPFLSHVVVSGPSASGEPRLAELM
                     ARAKNEFTVAPTRPDDACFWLYSSGSTGTPKGTVHVQTSMVATTELYAKPILGIEESD
                     VVFSAAKLFFAYGLGNGLSFPLSVGATTVLLGERPTPAAVCRILRQHQPTIFYGVPTL
                     YGALLASSELPNRAELNLRRCTSAGEALPPNVGRKWLEHTGVDILDGLGSTEMLHIFL
                     SNRPGDVRYGSSGKPVPGYEVRLLDEDGNKVATGEIGELQVCGPSSAAYYWNNREKSR
                     NTFLGSWTRTG"
BASE COUNT          819 a          997 c          971 g          841 t
ORIGIN      
        1 cactgacgtt tcccgcattg ccaggaaact gcaccatctc cgatgtgaga ctcgaatcat
       61 tcggatcaac gatattgtct atggtttgag ccatcttagt tctctccttt cgaatgttat
      121 gaatggccca gctatagcca aaatcaactc acaaatccag ggaagaatgg ctcgtttggt
      181 aaataaattg cgctccgtgc ccggcagtga acaccggaac ggggcgaatt cttcaacaga
      241 tcaaaaagga attcactgaa gataaaacgc cgcgctacag tgagcgcggc gccggtgaaa
      301 cttagctaat cggagctacg gtgtgaggtc aggtgggagt cgtggatgtc gacgaagact
      361 cggatttagc tggcacagat tctttctttg tctcggtttt cgattccttg ctctcggttt
      421 tcgattctga ggtcgacgaa cttgcgccgt tctcggtctt cggcgcatct ttacccttgc
      481 gcgcgtaatc cgtaatgtac cagccggtgc ccttaagctg aaacgaggtg ctcgagatca
      541 gcttctttac ctttcctttg caggtcggac acttggataa tggttcttcg gtgattctct
      601 gagtcgtttc aaaagttcca catttctggc actcatattc atagatgggc atgctaattc
      661 ctcggttacg atgctggttg caaagttaat cacggacagc gggcttgtca attaaacggc
      721 taggtcccgg aactttcgct cgtccgctgt tcgacttggt ttctgtcctt gccgtcggca
      781 agaatgtgcg cgccgccaaa ggcttcagaa cggttgccaa agagaaccag aggctcggtt
      841 ccagtattcg taatctggta ataatccttt gcgccgagaa acaacacgtc atgtttcttc
      901 acgggtcgct cgtcgccttt gatgcccttc accgtgcccg agccttcaag cacgagaaaa
      961 gtctcgggac tggcatgata atgcatgtcg gtgccgtcac ccggaacgaa gtaaagcatc
     1021 caggagcgga aattatcggt tccgaaaagg tcgatcttcc tcttcttgtt gtcacgcgct
     1081 tcggattgtt tttcgcccag gttaacaaaa tccatcgtcg ttcctccctt acggaaaata
     1141 ttagattcta gcctacagtc gcaacactcc gcaaggagac acgatctcag cttgacaggt
     1201 ttggagtgct gcactatgac agaggcgctt ccctcgttca tcgacgaaga gaagagtaac
     1261 ttcgaaatct cttggatatc cggcgaactg taaacgtcgg ccggcgaatt agaattagaa
     1321 gataaattag ccgatgggta cggacggtac ttcaacgaga ttctcggccg cggcagggga
     1381 atttctaagg tcaactacga tcacgtatcg aaccaccgcc gcatcattag caacaaaaca
     1441 gcgaaggtga gccatgacca gctcgtggga aagtactaac gtcgataaca gcaccatgcg
     1501 gctctatctc agtgtgccgg aacggggcgg cccggttccc ggcatcgtcg tcgtccacgg
     1561 acaatcagga ctggaaaatt ttatcaaaga agcgacacac atgttggcgc ttcagggcta
     1621 tgccgcaatt gcgccggacc tgtaccaccg cgacggtccg gattgcaaag acgataacat
     1681 gaccaaaaaa tcgagagtgc gcgattccaa catcatcgcc gacattaccg gcgcgatgca
     1741 gtttttgaag agccaccgcc aggtcgatgg tgcgcggttg ggcattgtcg gattttgcat
     1801 gggtggtcga atcgtctatc tgatgtcggc cgtcagtcgt gagctcaaag ccgctgtgat
     1861 gttttacggt agcagcacga tgacgccgtt cggcaagggt ccatcgccgt ttgatcgcac
     1921 acgagaaatc agctgccctc ttcaaggaca cttcggcgaa gaggatcaga atccatcgcc
     1981 cgaggacatg cgcaagctcg atgccgaact cacacgctgg aataagccgc atgagtttca
     2041 tacctaccag ggcgccgctc acgccttcgc caatgcaggt tccgccaact accggcctca
     2101 tgccgcggct ttggcatggc ctaaagccac agaatttttc agccggcatt taatcgggta
     2161 gagaaccgtc agtcgcgcgc tacgaatgaa gtgcgtgatg ccaaagatta tgcgcgttta
     2221 accagcatgc tatcggagca gccatcgcgc gcctctttgt tcatcatgcg catgtcgccc
     2281 gcccacatca cgagaaatcc tcagcgctgc catcagccgt cttgatggtt gcggcccttc
     2341 catagtaaaa aatgatgcca gccatggctg gcacgctagg agcgttaccg gaacaatccg
     2401 gattgcgagg aactgacgat gactgagaat tcagatgggt cggaatcggg tcgtcatcac
     2461 atgcatcact acaatgcagc agccgccttg attgaacgca acctcgctct gcgccctcaa
     2521 aaaattgcct acatcgacga caacggccgc tacacctttg ccgagctggc agaacgcgta
     2581 aatcgctgcg cccatgcctt ttgcgagctg ggtgccacgt tggaatcgcg catcatggtt
     2641 tgtcttagcg atacgatcga tttcccaagc gtgtttcttg gcgccatcaa ggcgggcctc
     2701 gttccagtcg cggtgaacac gctgctaacc agcggcgact acgatttcat gctgcgtgac
     2761 agccgcgctc gaatcctggt gttatccgag tcattactcc cagtattcaa accgatactg
     2821 gcgggacagc cgttcctgag ccatgttgtg gtctccggcc caagcgcttc cggcgagccg
     2881 cggcttgcag agttgatggc ccgcgcgaag aacgagttta ccgtcgcgcc gacccggccc
     2941 gacgatgcct gtttttggct ttactcttct ggctccacag gtacaccgaa aggcacggtc
     3001 cacgttcaga cgagtatggt tgcaaccacg gagctctacg ccaaaccaat cctgggaata
     3061 gaggagagcg atgttgtatt ctcggcggct aaattgtttt tcgcttacgg tcttggcaat
     3121 gggctaagtt ttccgctttc agtgggcgcg accacggttc tgctagggga gcggccgaca
     3181 cctgctgcgg tttgccggat tttacgccag caccagccga cgatctttta cggagtgccg
     3241 actctttatg gcgcgcttct ggcgagttcg gagctgccca accgcgcgga gctcaacctg
     3301 cgacgttgca cgtcggccgg cgaagccttg ccgccaaacg ttggccgcaa gtggctcgaa
     3361 cataccggag tcgacattct cgatggcctt ggttccaccg aaatgctgca tatcttttta
     3421 tccaatagac ccggcgacgt gcgctatggg agttccggca aaccagttcc aggttatgag
     3481 gtgcggctgc tcgatgagga cggcaacaag gtagccacag gtgagatcgg cgaattgcag
     3541 gtctgcggcc ccagcagcgc cgcgtactac tggaacaatc gcgagaaaag ccgcaacacg
     3601 tttctcggtt cctggactag gaccggcg
//
LOCUS       DASUTG010000105         5777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_312937, whole genome shotgun sequence.
ACCESSION   DASUTG010000105 DASUTG010000000
VERSION     DASUTG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312937"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1226
                     /locus_tag="VF089_02325"
     CDS             243..1226
                     /locus_tag="VF089_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6172823.1"
                     /translation="MKLISGLGHAISAAFIIQLFFGQPRAIAWEPTKPIEFVIPAGTG
                     GGADQMARLMAGLADKHKLSPRPFIVVNKSGGAGAEGFLHVKEKKGDAHTIIITLSNL
                     FTTPLATGTPFSWRDFTPVARMALDRFILWVNEETAYKSAKEYLAAVKQKPGMKMGGT
                     GSRQEDQIITVLLEQSQGVKFTYVPFKGGGEVCTNLVGNHIDSTVNNPAECVSNWKAK
                     RVRPLAVFDPERIPDKEWSGIPTIKEALGADISYNMLRGIFGPPDMPKEALEWYVGFL
                     KKIFETKDFQGYLQKGALKPAFATGSEYVKWVEENDKLHKELMEKGGLIKK"
     gene            1228..1707
                     /locus_tag="VF089_02330"
     CDS             1228..1707
                     /locus_tag="VF089_02330"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6172824.1"
                     /translation="MRALDLVTAAVLMLLSATVIYDALRIGVGWGLEGPESGFFPFWL
                     ATILTAVSVVLFVQALRSRSQKPFVTGERFVPVLKVLGPLAGFVILTDPPGPWSGLGL
                     YVAAGLYLGFYMRWVGRHHWHTVLALATAVPVLTFFIFETWFLVPMPKGPVETWLGY"
     gene            1717..3228
                     /locus_tag="VF089_02335"
     CDS             1717..3228
                     /locus_tag="VF089_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6172825.1"
                     /translation="MENLFLGFQIALTPYNLFIAVVGILLGTIIGVLPGLGGANGVAI
                     LLPLTFTMPPTSAIILLTCIYWGALFGGAITSVLFNIPGEPWSVATTFDGYPLAKAGR
                     SGEALTTAFTSSFVGAFFSIVLITFFAPLLAEVALKFGPPEFFAIQLLTFSSFVGLGG
                     GNPLKSLATILIGFMLSTVGLDIVTGQLRLTFGFTDLMKGFDFIIAVIGLFGIGEILL
                     SVEEGLRFQGAKTQMNMRVVLDTWKILPRFYRTFVRGSIIGFWMGFKPGGATPASFMS
                     YAFAKRFSKHPERFGKGELEGIVAPETAAHAAGVAAMLPMITLGIPGSPTAAVMLGGL
                     IIWGLQPGPMLFVDNPDFVWGLIASMYTGNVIGVLIVLAFVPFFASILRIPFAILTPL
                     IVVICAIGSYAVHNSMIDIWYMLIFGVIGYVFKKLDYPLAPLVLALVLGDMAENALRQ
                     SLIMSQGSLMIFLIRPISGVITAVAIFFFILPLLTPWWRKMRGLPQQRKPALE"
     gene            complement(3415..5577)
                     /gene="fdhF"
                     /locus_tag="VF089_02340"
     CDS             complement(3415..5577)
                     /gene="fdhF"
                     /locus_tag="VF089_02340"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX6172826.1"
                     /translation="MITPDRIVPTTCPYCGVGCNLELHIKDDFVYKVTSPFNTVVNHG
                     NLCVKGRFGYDFIYNKDRITTPLIRKTAQEPGARTQAFDRDQWREVSWNEALDYTTER
                     LVEIYRRDGADAMAVYCCAKATNEDNYLLQKLFRALFRSNNIDHCTRLCHAGSVVALQ
                     MALGSSAMSNTAAEVIHSDVFLVTGSNTAETHPIIALQMKAAVANHGAKLIVVDPRRV
                     EMVNWATLWLPEKPGTDVPLFSAMAHVIIKEGLYNRDFIERRTEGFEDFARSMEKFTP
                     AYAESISGVDRDLIVEAARMYATAKNAAIYWALGIPEHSHGTDNAMSLIHLALLTGHI
                     GRKGTGLNPLRGQNNVQGASDSGAMPWHYPGYQRVDDETAASKFEHAWNSEPGSLNRK
                     LGLTTTEIMSAVGPGGVRALHLMGENPMMSEPNLNHTRHKMEQLEFLVAQDLFINESD
                     AYADVFLPAASWAEKEGTFTNTDRRVQRVRKALEPRGQSRPDWQIICDLAQRIEKKLG
                     RQHTAFWHYQSSAEVLEEMGRVVPEYAGVKYWRIENQGLQTPVWDDSHPGTPYLFAET
                     FPSGKGKFHPLEYVPAVEMPDDEYPFILTTGRLLEHWHGGTLTRHSKLDELYPEARIE
                     INPADAVRLTIADGQTVRVSSRRGSIVLRVWVTQRATAGVVFIPMHFAEAAANLLTID
                     TLDPMAKIPEYKACAVRVVPATEDQLARPEGKVQRGRY"
     gene            complement(5574..>5777)
                     /locus_tag="VF089_02345"
     CDS             complement(5574..>5777)
                     /locus_tag="VF089_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6172827.1"
                     /translation="RGFDTGIATFFDKPMPETTCVFCGQCVGVCPTGALKPKRESLLE
                     QGFSLDEIMQATRGGKKRRRKSP"
BASE COUNT         1107 a         1614 c         1681 g         1375 t
ORIGIN      
        1 ccttacatga acatgacccg atcacttaag gcccgttgcc ccatacggta gcagcacttc
       61 attcaagtgc tggcttcaac tctgtcgctt ggcaagcttg cgttctcgtg ctgtgtgatg
      121 gctgttcgtc agaatctccc attgccgatc gcttgactga catgtggaat tctgattctt
      181 gataatcgcc aagcacagct tggatgtaac cgatacagaa gccgaaccaa aaggggggtt
      241 ctatgaagct tatcagcggc ttgggtcatg cgatttcggc agcttttatc attcagctct
      301 ttttcggcca accgagggcg atcgcttggg agcccaccaa gccgattgag ttcgtgatac
      361 cggccgggac ggggggcggc gccgatcaga tggctcgtct gatggccggt ctggctgaca
      421 agcacaagct ctcgccgcga cctttcatcg tggtcaataa atccggcggc gccggcgccg
      481 agggcttctt gcatgtcaag gagaagaagg gcgacgcgca cacgattatc ataaccctgt
      541 ctaacctgtt cacgacaccg ctggctaccg gcaccccatt cagctggcgc gatttcaccc
      601 cggtggcccg catggccctt gatcgtttca tcctgtgggt caatgaagaa acagcttata
      661 agagcgccaa agaatactta gcggccgtga agcagaagcc gggtatgaag atgggcggca
      721 ccggatccag acaggaggat cagatcatca ccgtgctgct ggagcagagc cagggagtga
      781 agttcaccta tgtgcccttt aaaggcggtg gcgaggtgtg caccaatctg gtcggcaatc
      841 acatcgattc aacggtcaat aatccggccg aatgcgtcag caattggaag gcaaagcgcg
      901 tccggccgct cgcggtcttc gatccagagc gtatccctga caaggagtgg agcggaatcc
      961 cgactatcaa agaggccctc ggcgcggata tctcgtacaa catgctgcgc ggcatcttcg
     1021 gccctcccga tatgcccaaa gaagccttgg aatggtacgt cggcttcttg aagaagattt
     1081 tcgagaccaa ggattttcag ggttatttac aaaagggggc tctcaagccg gcctttgcca
     1141 ccggtagcga gtatgtcaaa tgggtggagg agaatgacaa gcttcacaag gaattgatgg
     1201 agaagggtgg tttgatcaag aaatagcatg cgcgctttag atcttgttac cgccgccgtg
     1261 ctgatgcttc tgagcgccac tgtcatttat gatgctctgc gtatcggcgt cggctggggg
     1321 ctcgaagggc ccgagagcgg cttttttccg ttctggcttg ccactatcct gacagcggtc
     1381 agcgtcgtat tgttcgtgca ggctctgcga agtcgatcac aaaaaccttt tgtcaccgga
     1441 gaacggtttg tcccggttct caaagtactc gggcctctgg ccggcttcgt catcctcacc
     1501 gatcccccgg gcccgtggtc tgggctggga ttgtatgtcg cagcgggact ctatctcggc
     1561 ttctacatgc ggtgggtcgg gcgtcatcat tggcacacgg ttttggcgct cgccaccgca
     1621 gttcccgtcc ttacattttt catcttcgaa acctggtttc tggtgcccat gcccaaggga
     1681 cctgtcgaga catggctcgg gtattgacgc gtgggtatcg agaatctttt ccttggtttc
     1741 cagatcgcgc tgacgccgta taatcttttt attgctgtag tgggcatcct gctgggtacc
     1801 atcatcggtg ttctccccgg cctaggcggg gccaacggcg tggcaattct cctgcctctc
     1861 acgttcacta tgcctccgac atcggccatc atcctgctga catgcatata ctggggcgcg
     1921 cttttcggag gggcgatcac gtcggtgctc ttcaatattc caggcgagcc gtggtcggtg
     1981 gcgacgacat ttgacggcta tccgctcgcc aaggcaggta ggagcggcga ggcgcttacg
     2041 accgcattca catcttcgtt cgtgggggct ttcttctcga tcgtgctgat tacgtttttt
     2101 gccccgctcc tggctgaagt ggccctgaaa tttggccctc ctgagttttt cgcgatccag
     2161 ctcctgacat tttccagttt cgtcgggctc ggcggaggaa atccgctcaa gtcattggct
     2221 acgattctca ttggcttcat gctttctacc gtggggttgg acatcgtgac cggccagctc
     2281 aggctaacct tcggtttcac ggatctgatg aagggcttcg acttcatcat cgcggtcatc
     2341 ggtctcttcg gcatcggcga gattctgctc agcgtcgaag aggggctccg cttccaaggg
     2401 gcaaagacgc aaatgaacat gcgcgtagtg ctcgacacct ggaaaatttt accgcgcttt
     2461 taccgcacgt tcgtacgcgg ctcgattatc ggcttctgga tgggcttcaa gcccggcggc
     2521 gccacgccgg cgtcgttcat gagctacgct ttcgccaagc gtttttcgaa gcatccggaa
     2581 cgatttggca agggggagct cgaggggatc gtggcgcccg agaccgctgc gcatgcggct
     2641 ggcgtcgcgg ccatgctgcc gatgatcacc cttggcatcc caggctcgcc gacagcggcg
     2701 gtgatgctcg gtggactcat catctggggt ctgcagccgg gcccgatgct tttcgtcgat
     2761 aatccggact tcgtttgggg cctgattgcc agcatgtaca cgggcaatgt aatcggcgtg
     2821 ctgatcgtgc tggcgttcgt gccgttcttt gcgtccatcc tgcgcattcc tttcgcgatt
     2881 ctcacgcccc tgatcgtcgt catttgcgcc atcggctctt acgccgtgca caacagcatg
     2941 atcgacatct ggtatatgct gatcttcggt gtgatcggct acgttttcaa gaaactcgac
     3001 tatcctctag cgccgctggt gctggcgctt gtgctcgggg acatggcgga aaatgccctg
     3061 cggcagagtc tgatcatgtc tcaaggctcg ttgatgatct ttctgatccg tcctatctcc
     3121 ggcgtgatta ctgccgtcgc cattttcttt ttcatcctac cgttgttgac accatggtgg
     3181 cgtaagatgc gagggctgcc tcaacaacgt aagcccgcac tggagtagcc atgaagagca
     3241 cgaagttcag aaccttttat ttcaccgcat aatcttcgcg gcttatgtga ccgcggtgag
     3301 atgtatctac tcttaagcag taatgtagca ccactgccgc agaaatttgc cgggtaataa
     3361 ctatcaacca tgagtgcaga ctgcctagat ttcttgattt ggagactctt gccgttagta
     3421 tctccccctt tgaaccttcc cctctggcct ggctaattgg tcttctgtgg ccggtacgac
     3481 gcgcaccgcg caggccttgt attcggggat tttggccatg ggatccaatg tgtcgatggt
     3541 gagcaaattg gctgcagctt cggcgaaatg catagggatg aaaacaacac ccgccgtggc
     3601 ccgctgtgtc acccacacgc gcaggacgat ggaaccgcgc cgggatgaga cccgcaccgt
     3661 ttgtccgtct gcgatagtga ggcggacagc atccgcggga ttgatctcaa tccgcgcctc
     3721 gggatagagc tcgtcgagct tcgaatggcg ggtgagcgtg ccgccgtgcc agtgttcaag
     3781 caggcggccg gtggtgagga tgaacgggta ttcgtcatcc ggcatttcca cggccggcac
     3841 atactcaagc gggtggaact tacccttgcc gctggggaaa gtctcggcga ataaataggg
     3901 agtgccggga tgactgtcat cccagaccgg ggtctgtaag ccctgatttt cgatgcgcca
     3961 atattttacc ccggcgtatt caggaaccac acgtcccatc tcttcgagca cttcggcgga
     4021 actctgatag tgccaaaagg ctgtatgctg gcggccgagc ttcttttcga tgcgctgagc
     4081 aagatcacaa atgatctgcc aatcgggtcg ggactggccg cgcggttcca gcgccttgcg
     4141 tacgcgctgg acgcggcgat cggtgttggt aaacgttcct tctttctccg cccatgaagc
     4201 cgccggcaaa aagacgtccg cgtaggcgtc agactcattg atgaagaggt cttgcgccac
     4261 caaaaactcc aactgttcca tcttatggcg cgtgtgattc aaattgggct cggacatcat
     4321 cggattttcg cccatgaggt gaagagcgcg cacaccgccg ggtcctaccg cgctcatgat
     4381 ttctgtggtg gtcaggccca gtttgcggtt taagctaccc ggctcactgt tccaagcgtg
     4441 ttcgaatttg ctcgcggccg tttcatcgtc gactcgttga tagcccggat aatgccatgg
     4501 catggcgccg gaatccgacg cgccctgcac gttgttctgg ccgcgtaagg gattgaggcc
     4561 ggtgccctta cggccgatgt gtccggtgag cagcgcaaga tgaatcagcg acatcgcatt
     4621 gtcggtcccg tgtgaatgct cgggaatgcc caacgcccag tagatcgccg cgttctttgc
     4681 cgtcgcatac atgcgggcgg cctcgacgat cagatcgcgg tccacaccgg aaatagattc
     4741 ggcgtatgcg ggcgtaaatt tttccattga cctggcgaag tcttcgaaac cttcggtgcg
     4801 ccgctcgatg aaatcgcggt tataaagccc ctctttgata atgacatgcg ccatcgcgga
     4861 aaagagcggc acgtcggtgc ccggcttctc cggcagccaa agggttgccc aattgaccat
     4921 ctccacccgc cgtggatcga cgacgatgag cttggcgccg tggttggcga ccgctgcctt
     4981 catctgcagc gcaatgatcg gatgagtctc tgccgtgttg gagccggtga cgagaaaaac
     5041 atcgctgtga atgacttcgg cggcggtgtt gctcatcgcc gaggagccaa gcgccatttg
     5101 tagcgccacc accgaaccgg catggcacaa gcgggtgcag tgatcgatgt tgttgctgcg
     5161 gaacagcgcg cgaaacaatt tttgcaggag atagttgtcc tcgttggttg ccttggcgca
     5221 gcagtatacg gccatcgcat cggcgccatc gcgccggtaa atctcgacca gccgctcagt
     5281 ggtataatcg agggcctcgt tccaggatac ctcgcgccac tggtcgcggt cgaaggcctg
     5341 ggtgcgcgcg ccgggctcct gcgcagtctt gcgaatgagc ggcgtggtta tacggtcttt
     5401 gttgtaaatg aagtcgtagc cgaagcgtcc tttaacacag aggttgccgt ggtttacgac
     5461 cgtgttaaac ggcgaggtta ctttgtagac gaaatcgtcc ttgatgtgca gctccaggtt
     5521 gcaccccacg ccgcaatagg ggcacgtggt cggaacgatg cgatccggtg taatcatggt
     5581 gacttcctcc ggcgcttctt tccgcctctg gtcgcctgca tgatttcgtc cagcgaaaat
     5641 ccctgctcga gcagcgactc gcgcttgggc ttgagcgcgc cggtggggca gacgccgacg
     5701 cactggccgc aaaatacgca ggtcgtctca ggcatcggtt tatcaaagaa agttgcgatg
     5761 ccggtgtcaa agccgcg
//
LOCUS       DASUTG010000106         4961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_313038, whole genome shotgun sequence.
ACCESSION   DASUTG010000106 DASUTG010000000
VERSION     DASUTG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313038"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1165
                     /locus_tag="VF089_02350"
     CDS             149..1165
                     /locus_tag="VF089_02350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172828.1"
                     /translation="MANYKVIDADGHVRESVAGMREFLEPRWHRRNLFPNDEWDRDLH
                     GKLGQKPEGPEDQLSAMDQDGIDVMVLYPTAGLHVSALHEKDYATAITRAYNDWLFHF
                     CKRDSNRLKFVALLAPQDPAAAAEELKRAVKERGALAGVLPTYLPQMPDFGDGFYDPI
                     YAAAQQLGVGIGFHTGTSADSLGGQRFRKFLSAHTIDHPAEQMMALTATIIGGVFERF
                     PTLSIGFLESGIGWVPYMMDRLDEEVEKRGAVEAPYLTKLPSEYVRSGRIYFGVECEE
                     KTIPDAMRWGLEDTILYSSDYPHWDGDWPHTVKAIRDRKDLSDTTKQKMLHDNVVRFY
                     RFNE"
     gene            1290..2297
                     /locus_tag="VF089_02355"
     CDS             1290..2297
                     /locus_tag="VF089_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172829.1"
                     /translation="MPEWKMIDADGHIREVESEVFEYLPDHYKQRREALLYFPLLPHH
                     GWHRQAGRRSGAGSSFLIPSLQDWRTALEQGNIEAAVVYPTRFMHIGQVGIAEFAVEL
                     SRAYNDYLYDRFLRDEPRLKGVAVLPLQDINAAVAELRRAVREYGMVGGVLPADGLPR
                     PLGHPEFHPLYEEANHLGCMLAVHSQNSLRNNDLFLWRDEAATMAHVWPQMRQFTNLM
                     FSGVIGKYPDLRIAFLEAGCGWVPYLISKMEGRLGELARPMKLIERGQVYFQCGEEMT
                     TGRDLELLGDQCLFWASDFPHEGIVDMSKAVEEFLSRDDIPAASKKKISYDNAKRLYG
                     L"
     gene            2592..3974
                     /locus_tag="VF089_02360"
     CDS             2592..3974
                     /locus_tag="VF089_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6172830.1"
                     /translation="MATQSKEALTNLSGAIKGRVLGPDDSGYDEARQIWNAMIDRRPA
                     VIVQPANGDDVAPAIKFARENALAISVRGAGHNIAGNAISNGGLTIDFSQMKQVRVDA
                     AQKRAYADAGATLGDLDAATQRHGLATPVGINSTTGIAGLTLGGGFGWLTRKHGMTID
                     NLLAADVITADGKKLRASEQDNPELFWALRGGGGNFGVVTQFQFALHPVGPEILAGLL
                     AFPFAEAKQILTRYRQFASAAPEDLSVWVVLRQAPPLPFVPQNVHGKEVVVLPIFYAG
                     AATEGQRLIDELRAFGNPHGEHIGAQPYAEWQKAFDPLLTPGARNYWKSHNFTELGDG
                     AIDSIIEYADKLPSPQCEIFIGHIAGAANRVPPEAMAYAHRDAKFVLNVHGRWESTAE
                     DRRCIDWARSFFKASAPYASAGAYVNFMTEDEGERVTAAYGANYDRLAQLKKRFDPEN
                     VFHLNQNIKP"
     gene            complement(4071..4229)
                     /locus_tag="VF089_02365"
     CDS             complement(4071..4229)
                     /locus_tag="VF089_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallothionein"
                     /protein_id="HEX6172831.1"
                     /translation="MAEESCAHPDCDCAIEPGVGVSKGEKNYCSEFCAASEGASLEDC
                     GCGHSDCE"
     gene            complement(4543..>4961)
                     /locus_tag="VF089_02370"
     CDS             complement(4543..>4961)
                     /locus_tag="VF089_02370"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066362.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX6172832.1"
                     /translation="WGVECDCAFPSATQNELGSEDARILLRNGCGLISEGANMPSEPD
                     AVRQFIEMKICYGPGKAANAGGVAISGLEMSQNSLHYSWTREEVERRLQEIMRSIYTA
                     ASSTAAEYGQPANLVMGANIAGFVKVADAMIAQGVV"
BASE COUNT         1084 a         1359 c         1425 g         1093 t
ORIGIN      
        1 ggcggctttc ttgacactgt tcggagttcg gatcgttaga tagaggattc aatgcggaac
       61 ctaccctttt cagggttgca aaggagcgtg caggcggtgc ggatggggtg cgttcaatga
      121 aaccaaacgt tagagaaagg agtaggatat ggctaactac aaagttattg atgcggatgg
      181 ccacgtgcgc gagtctgtgg ccggcatgag ggagttcctg gaaccgcgct ggcaccgccg
      241 caatttgttt cccaacgacg agtgggaccg cgatctgcat ggcaagcttg ggcaaaaacc
      301 ggaaggaccg gaagatcaac tgtccgccat ggaccaagac ggcattgacg tcatggttct
      361 ttatccgacg gcgggtttgc acgtgagcgc gctgcacgaa aaagattacg cgacggcaat
      421 cacgcgcgcc tacaacgact ggctgtttca tttctgcaag agagattcga accggttaaa
      481 attcgtcgcg ctgctggcgc cgcaagatcc agccgccgcc gccgaagagc ttaagcgcgc
      541 ggtgaaagaa cgcggcgcgc tggctggcgt gctgccgacc tacctaccgc agatgcccga
      601 cttcggcgat ggcttttacg atccgattta cgccgcggcg cagcagcttg gcgtcggcat
      661 cggctttcac accggcacgt cggcggattc gctcggcggc cagcgctttc gtaaatttct
      721 ctctgcgcac accatagatc atccggctga gcagatgatg gcgctgaccg cgaccatcat
      781 cggtggcgta ttcgagcgct ttcccacact gagcatcggc tttctcgaat ccggcatcgg
      841 ctgggtgccc tacatgatgg accggctcga cgaggaagtg gaaaagcgtg gcgccgtcga
      901 agcgccgtat ctgaccaagc tgccgagtga gtatgtgagg agcgggcgga tttattttgg
      961 cgtcgagtgc gaagagaaga caattcccga cgcgatgcgt tggggattgg aagatacgat
     1021 tctttattcc tccgactacc cgcactggga tggtgactgg ccgcacacgg tcaaagcgat
     1081 tcgtgatcga aaagatctct ctgatactac gaagcaaaaa atgctgcacg acaacgtcgt
     1141 ccggttttac agatttaacg agtgatcctg gaagaggaaa caccgcagcc taagccatgg
     1201 tgcaaccggt ccataataag gctcgtactc tgaagtacta ttaattttgc tgataaccgc
     1261 cgagaagatg aatagaaaga gagttgtaca tgcctgagtg gaagatgatc gatgccgacg
     1321 ggcacatccg tgaagtcgaa agcgaggtct ttgaatacct gccggaccat tacaagcaac
     1381 gccgcgaggc actgctttac ttccctctgc tgccgcatca cggctggcat cgccaggcgg
     1441 gccgccgctc cggcgctgga tcgtcgtttc tgattccttc gctgcaggat tggcgaacgg
     1501 ctttagaaca ggggaatatc gaggcggcag tggtctatcc gacacgcttc atgcatatcg
     1561 gacaggtggg tatagctgag ttcgctgtcg aattgagccg cgcttacaat gactatcttt
     1621 acgatcgttt tttgcgtgat gaaccgcggt tgaagggagt tgccgttctg cccctgcagg
     1681 atatcaatgc ggcggtcgcg gaactgcgcc gcgcggtccg agagtatggc atggtgggcg
     1741 gggtgcttcc ggccgacggt ttgccccggc cgttaggtca tccggagttt cacccgctgt
     1801 acgaagaggc caaccacttg gggtgcatgt tggcggttca ctcacaaaat tcgctacgca
     1861 acaacgatct ctttctatgg cgcgatgaag cggcgaccat ggcccacgta tggccgcaga
     1921 tgcgccaatt taccaatctg atgttctcgg gcgtgatcgg aaaatatcct gacctccgca
     1981 tcgcattctt ggaagcaggt tgcggttggg tgccctatct gatcagcaag atggaagggc
     2041 gattgggcga gctggcgcga ccaatgaaac tgatcgaacg cgggcaagtt tactttcagt
     2101 gcggcgaaga gatgaccacc ggccgcgact tagaactgct cggcgatcag tgtcttttct
     2161 gggcttccga ttttccccac gaaggcatcg tcgatatgag caaggcagtc gaggaattcc
     2221 tatcgcgcga tgatataccc gccgcatcca agaagaagat cagctatgac aacgctaaaa
     2281 gattgtatgg tttataataa cgcctgtggg tactgctaca aatagcagcg gcataatgat
     2341 tcggcaagaa gtccggaatc tggcttgatg ctggctcttc gatatccggt gcttcgacgc
     2401 tactccggcc ggttcaattt ccctaattgg cttgactaag cgaagcgaaa atactacccg
     2461 aagggtaggg tatccacgcc tttgttttca ttcgcgcata tttcagtcgg gccttattac
     2521 acgtcagcgt cgcagcgttc tgcccggtaa tcaaagctac ttaatcgata acaaagatcg
     2581 gaggtaaacc tatggctacg caatcaaaag aggcattaac aaatctcagc ggcgcgatca
     2641 aaggccgggt gttaggcccg gacgattccg gttacgatga ggcgcgccaa atttggaatg
     2701 ccatgatcga ccgtcgtccg gcggtcatcg tacagccggc aaatggcgac gacgtcgcgc
     2761 cggccataaa atttgcgcgg gagaatgcgc tggcgatttc cgttcggggc gccggacata
     2821 acatcgccgg caacgccatc tccaatggcg gcctaaccat cgatttctca cagatgaagc
     2881 aggtccgggt ggatgccgcg cagaaacgcg cttacgccga tgccggcgca acgctcggtg
     2941 atctggatgc cgcgacgcag cggcatggct tggccacacc ggtcggcatc aactcgacga
     3001 ccgggatcgc cggcttgact ctcggtggcg gtttcggctg gctgactcga aagcacggca
     3061 tgaccatcga caatctgctt gcggccgacg tgataactgc ggacggtaaa aagctacgcg
     3121 cgagcgagca agacaatccg gagcttttct gggccttgcg cggcggcggc ggcaatttcg
     3181 gcgtcgttac ccagttccag tttgcccttc atccggtggg tcctgaaatc ctcgccggtt
     3241 tgctagcttt tccgttcgcc gaggccaagc agatcctaac ccggtatcgc caatttgcca
     3301 gcgcggcgcc ggaggatctc agcgtctggg ttgtgctccg tcaggcgccg cctctacctt
     3361 ttgttccgca aaatgttcat ggcaaagagg ttgtcgttct gccgattttt tatgcaggag
     3421 cggctacgga aggccagagg ctgatcgacg agcttcgtgc ttttggaaat ccccatggcg
     3481 agcatatcgg agctcagccc tatgcagaat ggcagaaggc tttcgatcct ctgctcacac
     3541 ccggcgccag aaattattgg aaatcccata acttcaccga gctcggcgac ggcgccatcg
     3601 attcgattat cgagtacgcc gacaaactgc cctctcccca gtgcgagatc ttcatcggtc
     3661 atatcgccgg cgccgccaat cgcgtgcccc ccgaagctat ggcttacgcg caccgggacg
     3721 ccaagtttgt tttgaacgtg cacggtcgat gggaaagcac agcagaggat cggcgctgca
     3781 tcgactgggc tcgcagtttc ttcaaggcca gcgcgcctta cgcttccgcg ggcgcttacg
     3841 ttaactttat gaccgaggat gagggtgagc gagtgaccgc cgcttacgga gcgaactatg
     3901 atcgtctggc gcaattaaag aaacgcttcg atccggagaa tgtttttcat ttgaaccaaa
     3961 atatcaaacc ataagatcga cggtttgttc taaatggatt agtccctcgg ctgttaagcg
     4021 aggcgcctct cacgatcgaa ccagaatacg tctgctaagg gtggcgctaa tcactcacaa
     4081 tctgaatggc cgcagccgca gtcttccaga ctggcgcctt cgctggcagc gcagaactcg
     4141 ctgcagtaat ttttttcgcc tttggacacg cctacgccgg gttcaattgc gcagtcacaa
     4201 tcaggatggg cacaagattc ttcagccatg attcattacc tccgcagttg taagaggcag
     4261 ttagcgtgcc acgacatcta cgacggcagg gcaccgacgt gtcccgatct ttagaggatc
     4321 gggctcgttt taatgcgatg tgtgtcttct tttcagtaca gaatgggaac gaatccgcat
     4381 aatgaattct acgtccggca gtactcagtt agtacggcct gcaggacccc gcatggttaa
     4441 ccgtcgctgc aaaaagaact ctgggtgaaa ccggcgaagg catcgcccgc gcaagcccac
     4501 ttctacttcc ctgaagtttc ctgatttcac aacggggata ccttacacga cgccctgggc
     4561 gatcatcgcg tcggcgactt tgacgaagcc ggcgatgttg gcgcccatca ccaggttggc
     4621 gggctggccg tactcggctg cggtgcttga agctgccgta tagatgctcc gcatgatctc
     4681 ctgcaagcgg cgctcgactt cttctcgtgt ccaactgtag tgaagggagt tctgactcat
     4741 ctccagtccc gagatcgcca cgcctccggc attggccgct ttgccggggc cgtagcaaat
     4801 cttcatttcg atgaattgac gcacggcatc gggttcggaa ggcatgttcg cgccttcgga
     4861 aatcagacca cagccgtttc tgagtaaaat cctggcatct tcgctgccca gctcattctg
     4921 cgtcgcgctg ggaaaggcgc aatcgcactc gacgccccag g
//
LOCUS       DASUTG010000107         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_905994, whole genome shotgun sequence.
ACCESSION   DASUTG010000107 DASUTG010000000
VERSION     DASUTG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905994"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..605
                     /locus_tag="VF089_02375"
     CDS             369..605
                     /locus_tag="VF089_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172833.1"
                     /translation="MIGKKIVLVSGISLFTLTLAIAGCGKKEEPAPPPPPPPPAAAPA
                     PAEPAPATPTPGGDAGAPATGEKAPETKDEAKTK"
     gene            695..1423
                     /gene="tatC"
                     /locus_tag="VF089_02380"
     CDS             695..1423
                     /gene="tatC"
                     /locus_tag="VF089_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016647029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6172834.1"
                     /translation="MVSDSYLTFTEHLEELRSCLLRALAAVVVASLACYFFADAIFGF
                     MVAPLKQILPPGQNLIGTSVTEAFFTEIKVSLLAGVFFSSPLIFYEVWRFIRPGLIGN
                     EGRLVIPFVLSATLFFLGGAYFCYRIVLPVAFSYFIEQYGSLGVTPAIRLGEYFTFFF
                     RMVLAFGITFELPIITFVLVRLGVWDHRFMRRSFRYAVIVIFILAAFLTPTPDIINQL
                     LLALPMLLLYIVSIGVAYIWRKSE"
     gene            1457..3580
                     /locus_tag="VF089_02385"
     CDS             1457..3580
                     /locus_tag="VF089_02385"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX6172835.1"
                     /translation="MVAFVIAVSFLSLVFAVYLARWVLARDTGTPKMREISDAIKEGA
                     EAFLSRQNRTIIFMSAALAALIFFLYAYVRAPSPADPVSPVEMAFWTTFSFVLGALCS
                     VIAGYVGMWVSIRANIRTASAVRTSLNEGLRIAMRGGAVSGLFVVAMSLLGVGGLYAL
                     LEFLGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGLYPYFGIKGIVFPLVA
                     RALGLLASIVGIMIVKTDENGDPMHALNRGYYVTSGLAMVGFFVCAQWLLSVDPAKYP
                     EAQSAWFYFFLCGVIGILMAQAFVYITQYYTEYKYRPVREIAEASQTGPATNIIAGVA
                     VGFECVAAPVVVISIAIIASYYLGLNSGVKNAGLFGTAVATMGMLGTAAYILAMDTFG
                     PITDNAGGIVEMSGQPEEVRHKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYLD
                     EVAHYGHPLESVNIAKPEVFVGALLGAMLVFLFSAYAIRAVGKAAYYVINEVRRQFKE
                     NPGILQGTVKPDYRRCVDIVTVGALKQMILPGVVAVTMPIAVGLVFRSLGVGAEAVAA
                     FLMVGTIAGILVATFLNNSGGAWDNAKKYIETGAYGGKGSEPHKAAVVGDTLGDPFKD
                     TAGPSLHVLIKLLATITLVLAPLFV"
     gene            3655..3744
                     /locus_tag="VF089_02390"
     tRNA            3655..3744
                     /locus_tag="VF089_02390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3690..3692,aa:Leu,seq:taa)
     gene            4355..4534
                     /locus_tag="VF089_02395"
                     /pseudo
     CDS             4355..4534
                     /locus_tag="VF089_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
BASE COUNT         1051 a         1236 c         1373 g         1299 t
ORIGIN      
        1 acagtttatg cacaaaaact gtggccaatc tcatgctacg cggaaccggt taaatgcgtt
       61 gacggcggca ggctcgtctt gcgcatcatc tactgctaac aataccagag agcgtatctt
      121 gagttttgag tttatagtct cgggcatcgt atctctgctg ctgttaaaca gagccggcca
      181 aatcgtcaag ctcgagtgaa agatcgcgca cagattcttg ctgggactct ctctgcaatc
      241 gtcgttgacg gatccggaga gcgctctcaa gggcttaaaa agccttgcat accggacgga
      301 gttatgtaat aaaatttctt tagttaacgt tttctttgta gacgtgtgac gggaaaggag
      361 gtgaactaat gatcggtaag aaaattgttt tagtgtcagg aatatcgctc tttactttga
      421 ctctcgcgat tgcagggtgc ggcaagaaag aagagccggc tccaccgccg cctccgcctc
      481 caccagctgc agcgccagct ccggcggaac cggcaccagc aactccgaca ccgggtggcg
      541 atgcaggggc tccagcaacc ggtgaaaaag ctcctgaaac gaaggacgag gcgaaaacca
      601 agtaaagttt caactcctaa caggttattt gccaaccaaa aaggggaagg gtaatcatcc
      661 ttcccctttt tgtaatttgc gccgtggaag gcttgtggta tcggacagtt atttaacttt
      721 tacggagcat cttgaggagc tacgttcgtg cttgctcagg gcgttggctg cggttgttgt
      781 tgcttccctg gcctgctatt ttttcgccga tgcgatcttt ggttttatgg tggctccgct
      841 caagcaaatt ctgcctcctg gccaaaatct aattggaact agcgtcaccg aagcgttttt
      901 caccgaaata aaagtctcgc ttttagccgg cgtgtttttt tcctctcctc ttatttttta
      961 cgaagtctgg cgtttcatca gaccgggcct gatcggcaat gaaggcagat tagtcatccc
     1021 tttcgttttg tctgcgacgc tattttttct cggcggcgcc tacttctgtt accgaattgt
     1081 ccttcccgtg gcgttttcct actttatcga acagtacggc agcttggggg tgacgccggc
     1141 aatccgctta ggcgagtact ttactttttt ttttcgtatg gtgctggcct tcggaatcac
     1201 gtttgagttg ccgattatca catttgttct cgtccggttg ggcgtctggg atcaccgctt
     1261 catgcgacgg tcctttcgtt atgcagtcat tgtgatattc atacttgctg cgtttttaac
     1321 gccgacgccg gatatcatta atcaattgtt gttagcccta ccgatgctac tgctatatat
     1381 cgtgagtatc ggtgtcgcgt atatctggcg taaatctgag tgagcgtcgt tgtcattcat
     1441 cggacgagga ggattgatgg ttgcatttgt tatcgccgtt agctttttgt cgctggtatt
     1501 cgccgtctat ctagcgcgtt gggtgctggc gcgcgacacc ggtaccccga agatgcgaga
     1561 gatatccgat gccatcaagg aaggcgctga agcttttctc tcgcggcaga accgcacgat
     1621 tatatttatg tccgcggcct tggcggcgct gatctttttt ctttacgcat acgttcgcgc
     1681 gccgagtccg gcagatccgg tttcaccagt agaaatggcc ttttggacga cgttttcctt
     1741 tgttctcggc gctctttgtt ccgtaatcgc cggctatgtc ggcatgtggg tgtcaattcg
     1801 agcgaatatc cgcaccgcat cggcagttcg cactagtctc aacgaagggc tgcgcatcgc
     1861 aatgcgtggt ggcgcggtgt caggcttatt tgtcgtcgcc atgagtttgc tcggcgtggg
     1921 aggtctttac gcgctgctcg agttccttgg gcacgatccg cagaagattc cattcacgat
     1981 tgttggctac ggctttggcg catcctttgt cgcgcttttt gctcaactcg gcggcggtat
     2041 ttataccaaa gcggctgatg tcggcgccga tttggtcggt aaagtcgaag ccggcatccc
     2101 tgaggacgat ccgcgtaacc ccgccgtcat cgcagatctg gtcggcgaca atgttggcga
     2161 ctgcgccggt cgtggtgccg atttgttcga atccactgcg gcggagaata tcggcgcgat
     2221 gatccttgga gtgggcctct atccgtattt cggcatcaaa ggaatcgtct ttcctttggt
     2281 cgcccgcgca ctcggtctcc tcgcctcgat cgtaggtatc atgatcgtta aaacggatga
     2341 gaacggcgac ccaatgcacg ctttgaaccg gggatattac gtaaccagcg gcttggcgat
     2401 ggtgggcttt tttgtctgcg ctcaatggct tctatctgtc gatccggcga agtacccgga
     2461 agcgcagtcc gcgtggtttt acttctttct ctgcggcgtc atcggcattc ttatggcgca
     2521 ggctttcgtc tatatcacgc agtactatac tgaatacaaa tatcgtccgg tcagagaaat
     2581 agcggaggcg tctcaaaccg gaccggcaac gaacattatc gcgggggtcg cggttggctt
     2641 tgagtgtgta gcggcgccgg tagtcgtcat ctcgatagcg atcatagctt cttactattt
     2701 agggttgaat agcggcgtca agaatgccgg ccttttcggc accgccgtcg ctaccatggg
     2761 aatgctggga accgctgcct atattcttgc catggatacc tttggtccaa tcactgacaa
     2821 tgccggtggc atcgtcgaaa tgagcggaca gccggaggag gtccgtcata agaccgatcg
     2881 tctggacgcc gtaggaaata ccaccaaagc cttaaccaag ggttatgcca tcggatcggc
     2941 ggccctcgcg gcattccttc tattctccgc ttatctcgat gaagtggccc actatggcca
     3001 tccactagag agcgtcaaca ttgccaagcc ggaagtattt gtcggcgcac tgcttggggc
     3061 aatgctggtt tttctttttt ctgcctatgc gattcgcgca gttggcaaag ccgcctacta
     3121 cgtcatcaac gaggttcggc ggcagttcaa agaaaatcca ggcatccttc agggcaccgt
     3181 aaaacctgat tatcgccgct gtgtcgatat cgttacagtg ggtgcgttaa agcagatgat
     3241 ccttcccggg gtggtcgcgg tgacgatgcc gattgctgtc ggcttagtgt tccgctctct
     3301 cggagtaggc gccgaggcgg tcgcggcatt cctaatggtg ggtaccatag ccggtattct
     3361 cgttgctacc tttctcaaca acagcggggg ggcctgggac aatgccaaaa aatatatcga
     3421 aaccggtgcc tacggcggca agggttcgga acctcacaaa gctgcggtcg ttggagatac
     3481 gttaggtgat ccgtttaaag atactgcggg tcccagtttg cacgtgctga tcaaactact
     3541 ggcgacaatt acgctcgtgc tggcgccctt gtttgtgtaa gcggcgtctt tgaactttct
     3601 cgttgggctt gtgcttgcta tcgcagaata taactgacca ttggttttat taccgcccgg
     3661 atggtggaat ttggtagaca cacgggactt aaaatcccga ggaccgaaag gtccgtgccg
     3721 gttcgagtcc ggctccgggc accaacatac ctggtagagc ccatctgcgc gttcacacca
     3781 agccaggctt ttctatcttg agagctgcga ttagtgcatt tagaatgttg tgtctcgagc
     3841 gatagcaaag agaaagcagt ggtgctgatc tgcgtgcctt caagtattaa ggtggtgaaa
     3901 aggaggtagt tttaacatgc ctcagtatga aatccacgtg ctttgcagtg agtgtggtgc
     3961 cgaacatccg atgcgtgttc ggcttcacct cgatgacggt ccggtcgata agcgcagcat
     4021 cagtgaaact tacaagggaa gaaaccagcc tccccagctc ctagccgttg gcggacacaa
     4081 aacattgtgc ctaaaaaccg ggaagatgtt tgtacaggag aatgaggacc aaatcttctt
     4141 gatacctcga tgaactgttt agactcctac atcacgttgc ctctcgagga tttcccgttg
     4201 atgggacagc ctcgggcggt cccgtcgcag aactggacaa gttctcaaag ccttttcgta
     4261 cgttttctaa cccgtgggag tggcatgtgc tttgcgcgat aatcaaatct cccacaagca
     4321 gcattcgtcg atcaaatcag aggagggtta tactatgaac gaagatgttt tctcggggaa
     4381 atggagagaa atgcggggca cattacgctc ctggtggggc aggctgagcg acgatgattt
     4441 agagagaatc ggcggagaga aagacaaact ggtcggcctg gtacaggaga ggtacggcca
     4501 aactcgcgag caggcggaga gagaagttga tcggcgcctc tccgagtatg gaagcagcgg
     4561 tgggattagt tccaacctaa gatctcgagt cgatgaactg ggaagtacgg taaccaccaa
     4621 agccggtgag gcggcagggg cgatggccag cggagtgcaa tcggcccggt cctacctgca
     4681 ggagcgggat ttcgaagaat tgaccgggga catagtcgct tgggttcgtc gctacccaat
     4741 tcaagcctgt ttgatcggca tcggtttagg ctacttgata gcgcgtagca tgggcggcag
     4801 tgatcgcgca gattacgaat agagctcacc cctttcattg ggttaccgcg ggctgatcat
     4861 ttgctccggc gaaacctgtc tatcctaact gcaaatgatg ccccgtcgcc caccgacggg
     4921 gcatccattt tgtttgtgta tttacaccgg aatcaagaa
//
LOCUS       DASUTG010000108         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2092563, whole genome shotgun sequence.
ACCESSION   DASUTG010000108 DASUTG010000000
VERSION     DASUTG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092563"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF089_02400"
     CDS             <1..345
                     /locus_tag="VF089_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172836.1"
                     /translation="EAERVLEGIYVAGWARNASVGLVGVAKQDAERGMKVVNEYLRAK
                     EGFSSGEMEAKIEAILNILDKRGVSFVTKEDVEILESIEHEEARNRKTWDFKFISDDQ
                     MLQVIRERKAAR"
     gene            1356..2345
                     /locus_tag="VF089_02405"
     CDS             1356..2345
                     /locus_tag="VF089_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HEX6172837.1"
                     /translation="MDTEGNIKRSVIGVLLCSVLLGFAASWVWSAATDASKPASATYV
                     GNEVCQACHAPQFEKFSTTLMGKIFLHNPRNETESRACENCHGPGSEHVAAGGGKGVG
                     GMITFRKDSGESAEVQNEPCLGCHERGLQTYWKASTHAARGLSCVNCHTLMEKTSGKQ
                     MAKLTEVTPFFINRAEIEVCGQCHLQRKAQLLRSSHMPLREGKMVCSDCHNPHGSPNP
                     AQLKQRSVNENCYTCHAERRGPFLWEHPPVLENCSNCHEPHGSVNDRLLKVTDPRLCT
                     QCHIAVRHPGTPRPLSRLSPTVGPSVFAYNRSCTNCHSQVHGSNHPSGKFYTR"
     gene            2361..>3230
                     /locus_tag="VF089_02410"
     CDS             2361..>3230
                     /locus_tag="VF089_02410"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEX6172838.1"
                     /translation="MRCRKAALLLSVSAITLLFLVAPSAAQRDLGNYTVSGEAEVGGL
                     VRHKSGNNTKFEEYRDLPETVIVPQLELFIDSKKNDFYLEFGSIKPGLDDQSYRLRAG
                     RYGLVDLEFVWDQIPHLFNVDNARTPYNMSGGNYTLRSKPPGATGAEVRDWVNAEARP
                     VDLKLYNGLARFSVRYTPTPGWTFSGSYGSQNTAGKRAFGLYFGPSPGSFNITELAEP
                     IDYQTHNIELGGEYAGAGWSLGLRYNGSLFHNNTSTLVWDNPMNPGIGGACTDEAVYN
                     NATRTGPCRGRADL"
BASE COUNT          777 a          846 c          899 g          708 t
ORIGIN      
        1 gaagccgaaa gggtgctcga gggcatttat gttgccggtt gggcgcgcaa cgccagcgtc
       61 ggcctagtcg gggtcgctaa gcaggatgcc gagagaggca tgaaggtggt taacgaatac
      121 ttgagggcca aggaaggatt ctcgtccggt gagatggaag ccaagatcga agccattctc
      181 aatatccttg acaagcgagg cgtttccttc gtgactaaag aagacgtgga gatccttgag
      241 agcattgaac acgaggaagc ccgcaacaga aaaacctggg attttaaatt catcagcgat
      301 gaccagatgc tgcaggtgat tcgagagcgc aaggcggcgc gctgacatac ttcttataat
      361 cttgtagcct taattgaggg ccgcggtgat tatcttcccc gcctagcggt tgcctttagt
      421 acagctttcg tgttccccct gatgtagacg caaggaagac ccaaagcagt tcaggatccc
      481 cagtgttcat tcaatctcgg aatagaggac gaagctcgga ctataatccg ggcaaagaat
      541 ggctatagcg gcagtaactc ccgtgagatt gccggccgct cggaagtgca ttatccctgt
      601 cccagccttt acgctgcggc gttatgatca aaagaaattg aggacggcca aggcggggtg
      661 gaggaagctt gacatcataa ccgcgccgct tgagcttttt cgctcgcgct aacgctgcta
      721 gccagttttc aactgattcg cgccttcggt ctgcgatcat ctgctttgaa ttttttgctt
      781 gatgaggatg atgtcttttc ttcgtttcaa gtttgttctc ccaaccggct gaaccactcg
      841 attttgcagc cgctttaacg caatgaaagg gccatggaca agagtaggtt tatcttgtcg
      901 gtctttgaaa cctcggcgag cgctaaagca gttcatgttc catcgttttc ttatctttac
      961 gaattctagc tccgtttttt gcactcacag gaaagaggct tgggatcgat acagaatgat
     1021 caaagccgag aacggcatta agtcctgcga aagtttcgat gattcgcgga gggagagctt
     1081 caactaatgc acggcgcagc gacagttaaa gagtaggtgt accggaaaac ctttcgcgat
     1141 catcgatgcc gcttgaaatc ctcataacgg cccaaatcgg attaaagaat ggtcggaatc
     1201 atcgtgttga caaacgcacg ggcatcatct agcagcgggc ggtaatgcgt taagcggcag
     1261 ttgattgcga aattccgcga aagaatctag gtcgcgattt ttttctagac aaaccgttcc
     1321 tcgcagtggt atagcgcatt cgattctgtg caacgatgga caccgaagga aatatcaagc
     1381 gatcagtgat cggtgtgcta ttatgcagcg tgctgttggg cttcgctgcc tcatgggttt
     1441 ggtccgcggc aacggatgcg tcaaaacccg cctctgccac ttatgtcggc aatgaagttt
     1501 gtcaagcttg ccacgcgccg cagttcgaaa aattctcgac aactttgatg ggcaagatat
     1561 tcttgcacaa cccgcgtaac gaaaccgaaa gtcgggcgtg cgagaactgc catggtccgg
     1621 gaagcgagca tgtcgcggcc ggcggcggca aaggcgtcgg cggtatgatc acgttccgga
     1681 aagattccgg cgaatccgcc gaagtacaaa acgagccctg cctcggctgc catgagaggg
     1741 gccttcagac ctactggaag gcgagcactc acgctgcgcg gggcctttcc tgcgtcaact
     1801 gccatacgtt gatggagaag accagcggca agcagatggc gaagctcact gaggttactc
     1861 ctttcttcat taatcgggcg gagattgaag tttgcgggca gtgccatcta caacgcaagg
     1921 cgcaactgct tcgctcttcg cacatgccac tcagagaggg caagatggtc tgttcggact
     1981 gccacaaccc gcacggaagc cccaatccgg cgcagctcaa acaaagaagc gtcaatgaaa
     2041 attgctatac ctgtcatgcg gaacggcgcg gaccgtttct ctgggagcat ccgccggtgc
     2101 tggaaaattg ctccaactgt catgaacccc acgggtcggt caacgaccgg ctgctgaaag
     2161 tcaccgaccc gcggctatgc acgcaatgtc atattgctgt tcgacatccg ggcacgccgc
     2221 ggccgctcag tcggttatca ccaaccgtcg gtccgtcggt tttcgcgtat aatcggagct
     2281 gcaccaattg tcatagccag gtccacggca gcaatcaccc atcaggaaaa ttctacaccc
     2341 gttagggacg gaggtcggct atgagatgcc gtaaagccgc tttgctttta agtgtaagcg
     2401 ctatcactct cctgttcctg gttgcgccca gcgcggcgca gcgcgatcta gggaattata
     2461 cggtcagcgg agaagcagaa gtcggcgggc tggtaaggca caagagcggg aacaatacaa
     2521 aattcgaaga gtaccgtgat cttccagaga ccgtgatcgt ccctcaactc gagcttttca
     2581 tcgatagcaa aaagaacgat ttctatctgg agttcggttc catcaaaccc ggcttagacg
     2641 accaaagcta tcgtttgcgt gctggacggt acggccttgt cgatctcgaa ttcgtctggg
     2701 atcagattcc gcatctgttc aatgtcgata acgcgcgcac cccatacaac atgagcggcg
     2761 gcaactacac cctcaggagc aaaccgcctg gggcaacggg ggcggaggtg cgcgactggg
     2821 tgaacgccga ggcaagaccg gtcgatttga agctatacaa tggcctggcg agattcagcg
     2881 tacgttacac gccaacacca ggatggactt tctccggcag ctacggctcg cagaataccg
     2941 ccggcaaacg cgccttcggc ctgtactttg gcccgagccc cggcagcttt aacattaccg
     3001 agctggcgga gccaatcgat tatcaaacgc acaatattga gctcggagga gaatacgcgg
     3061 gcgccggctg gtctctaggc cttagataca atggatcgct gtttcacaat aacaccagca
     3121 ccctggtctg ggacaatccg atgaatcccg ggatcggcgg agcgtgcacc gatgaagcgg
     3181 tttataataa cgcgacgcgc acaggcccgt gccgcggtcg tgccgatctc
//
LOCUS       DASUTG010000109         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_906366, whole genome shotgun sequence.
ACCESSION   DASUTG010000109 DASUTG010000000
VERSION     DASUTG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906366"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VF089_02415"
     CDS             complement(<1..337)
                     /locus_tag="VF089_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6172839.1"
                     /translation="MKPAGQEAPEPRQDESKADLQDLLPTQRDLDIRSITLTGLFVLA
                     TFYTLYFARDFILPILLAWILSSLLAPVVRSLKRIHIPEPLSALLIISALLGTLSLGI
                     YRMSEPVASW"
     gene            complement(710..1423)
                     /locus_tag="VF089_02420"
     CDS             complement(710..1423)
                     /locus_tag="VF089_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6172840.1"
                     /translation="MKSFRILVVEDNQDLLDLIVKYLELCGWRASAANRQEDFWLELE
                     RHRPNLILLDVTILGSETSSLLSDFRSDLRFTDIPVVAMTVMGSTRAKSLCITAGCDR
                     VLAKPFHLDELRRLVEWFADKQKNTSKTGGIIMKKPTRHFAAVALLAFALSGCQAMTG
                     RTAGQNVDDARITTAVQATLTSDKASNFTRIDVDTTNGVVYLNGTVQSAEQKARAEQL
                     AGRIDGVKKVVNNLQVGKS"
     gene            complement(1505..1669)
                     /locus_tag="VF089_02425"
     CDS             complement(1505..1669)
                     /locus_tag="VF089_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX6172841.1"
                     /translation="MLSWAITFLIIGLIAGILGLTGIAGAATQIAWILFVVFLVLFVV
                     SLVMGRRSGV"
     gene            complement(1757..1894)
                     /locus_tag="VF089_02430"
     CDS             complement(1757..1894)
                     /locus_tag="VF089_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX6172842.1"
                     /translation="MQFYISLQPLIALIAGILILLVPRLLNYVVALYLIITGILGLTR
                     M"
     gene            complement(1912..2523)
                     /locus_tag="VF089_02435"
     CDS             complement(1912..2523)
                     /locus_tag="VF089_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172843.1"
                     /translation="MKPQPVMTAPRAIFTLAAVSAVPMVHWSCEDVALVGRPALDARS
                     SRENVEFIGRVQELDHSRRELYIRTEGGQSQIVTYTNDTRVAADGKAISNSELGRGDV
                     IEVRMHGTADGRAVADSILVRERGDRNTTIEGTVERVLGDRRVIELRTASGALTRVYV
                     PQGSPETVENEFKKLRAGDFVRFQGIFLGENRFELTRVVKPAE"
     gene            complement(2538..>3177)
                     /locus_tag="VF089_02440"
     CDS             complement(2538..>3177)
                     /locus_tag="VF089_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6172844.1"
                     /translation="TLVSGCVVADGAAPRGDSRNDDRRQPANTRQIDSRQAERFYRIM
                     TPLLKNMDRPKSPKDVRIGIIDEADINAANAGGGEFYVTTGLLEKASDEQLRGIMAHE
                     IAHDDLGHVAKAQGLGVGLNLGVILLEQLIPGSSAVTPIAGSLIARGYSRNEEYAADR
                     HGVEILRRAGYSKDVLINALNWVTRTSGGGGGGFLSTHPATEDRIAQLEKLR"
BASE COUNT          730 a          938 c          804 g          705 t
ORIGIN      
        1 tccaggacgc aaccggctcc gacatacgat agatgcccaa agacagtgtg cccaggagcg
       61 cggagataat tagcaaggcg ctcaatggct cggggatgtg aatgcgtttg agagatcgca
      121 cgaccggcgc caaaagagag ctcaaaatcc acgccagaag tatcggaaga atgaagtcac
      181 gcgcaaaata gagcgtgtag aaagtcgcta gtacaaaaag accggtgagc gtaatcgagc
      241 gaatatccag atctcgttga gtgggcagca agtcctggag atcggccttg ctttcatcct
      301 gccgaggctc gggcgcttcc tgcccggcag gcttcatggc acaacctggt tatcgctcca
      361 cgtgttgaca gaccgcgccg cccacatgag gccaggaaca ggcataaccg ttgccgtgag
      421 tttcttaggc gggcgcttgg gcggtggatc cccggctgga ggcgggtctc gcggatctga
      481 tatctccggt tcgcgagaag gtggctcatc gacgggcggc ttctttcgcc ggtttggggg
      541 ttcccgcctg ggttcgggcg gtctcttagc taacccggca tggagcacga atccgacagg
      601 gcatgttgcc tgccacctca gatcatggtc gttgtccatc gattgctccg ccaatcagcc
      661 acctgtccga accgagatga gaacccaaaa tacaattcca ccgaatgatc taggatttac
      721 ccacctgaag gttgttgact accttcttaa caccatcaat gcgaccggct aattgctcgg
      781 ctcgcgcctt ctgctcggcg ctttgtaccg tgccgttcaa gtaaaccacg ccattggtgg
      841 tatcgacgtc gatacgagta aaattcgacg ctttgtccga cgttaaggta gcctgtaccg
      901 cagtcgtgat gcgtgcgtca tcgacgttct gccctgccgt tcggccggtc atcgcttggc
      961 acccggaaag cgcgaaagcc aaaagcgcta ctgcggcaaa atgtctagtc ggtttcttca
     1021 tgatgattcc tcctgttttg gacgtatttt tctgtttatc agcaaaccac tcgaccagcc
     1081 ggcgcagctc gtccaagtga aacggtttgg ctaaaacccg gtcacatcca gcagtgatac
     1141 agagactttt agcccgcgtt gagcccatca ccgtcatcgc cacgacgggg atatccgtga
     1201 accgcaaatc gcttctaaag tcgctaagca acgaggaagt ttcactgcct agaatggtta
     1261 cgtcaagcaa tatcaaattg ggtctgtgcc gctcgagctc tagccagaag tcctcctgcc
     1321 tgtttgctgc cgacgcgcgc caaccgcaaa gctccaagta cttcacgatc agatcgagaa
     1381 gatcctgatt gtcctcgacg acgaggatgc gaaaagattt cacagtaact cacctcggca
     1441 gtccaaagct ccggacgcag cgcaactctc gttacctgcg acttcagcaa ttgtatggcc
     1501 cgcctcaaac ccctgagcgg cgacccatta ctaaactgac gacaaacaaa accaggaaca
     1561 ctacaaacag gatccaggcg atttgtgtcg ctgctccagc aatcccggta agtcccaaga
     1621 tccctgcgat cagcccgata atcaaaaaag taattgccca acttaacatg gctctatcct
     1681 cctgcacgtt tttttccact ttagaacagg gtactcttca aattgtttga tcttgtcgaa
     1741 tccgtggatc tctcctctac attcgcgtca gcccaagaat cccagtgata atcaaataaa
     1801 gggccacaac ataattcagc agtcttggaa ccagcaaaat caggatcccg gcaatcaagg
     1861 cgatcagcgg ctgcaaggaa atgtaaaact gcattgataa ttctcccttc gtcactcggc
     1921 cggtttaacg actcgtgtta attcgaaccg gttctcgcct aagaagatgc cttgaaaccg
     1981 aacaaaatcg ccggcgcgta attttttaaa ctcattctcg actgtttcag gagagccctg
     2041 cggcacatac acgcgggtta gagcacccga ggcggtacgt agctcgatga cgcggcgatc
     2101 accgagcacc cgctcaacgg ttccttcgat agtcgtgtta cgatctccgc gttcacgcac
     2161 cagaatcgaa tccgccaccg cgcgcccgtc ggctgttcca tgcatccgaa cctcgatgac
     2221 gtcaccgcga cctagttcgc tgttcgagat tgcctttccg tcagcggcga cccgagtgtc
     2281 gtttgtatac gtaacgatct ggctctgccc tccttcagtg cgaatgtaaa gctcgcgacg
     2341 gctatgatcc aactcctgaa ccctgccgat aaattctacg ttctcccggc tggagcgtgc
     2401 atcgagcgca ggtctcccca ctaaggcaac atcttcacag ctccaatgga ccatgggcac
     2461 tgcggaaacc gcagctagag tgaaaatggc gcgaggggcg gtcatcactg gttgtggttt
     2521 cacgcgcggg tcctttgcta acgcaatttt tctagctgcg cgatacgatc ttccgtggct
     2581 ggatgggtcg ataggaagcc gccaccgccg cctcccgagg ttcgagtcac ccagttcaac
     2641 gcattgatca agacatcttt cgaatatccg gctcgccgca agatttcgac accatggcga
     2701 tcggcagcat actcttcgtt ccggctatag ccacgcgcga tcagactccc cgcgatcggc
     2761 gtcaccgcgc tgctgccagg tatgagctgc tcgagaagta tcactcctaa attcaagccg
     2821 acgccaagac cctgagcctt ggcgacatgg cctaggtcat cgtgagcgat ttcatgggcc
     2881 atgatgccgc gcaactgttc gtcgctggcc ttctccaaca gaccggtggt cacgtagaac
     2941 tccccaccgc cggcattcgc agcattgatg tcggcttcat cgataatacc gatcctcaca
     3001 tcttttggcg atttcggacg gtccatattc tttagcagcg gtgtcatgat gcgataaaac
     3061 cgctcggctt gccgagaatc tatttggcga gtattcgccg gctgacgccg atcatcgttc
     3121 ctactatcgc cccgcggcgc tgcaccatcc gcgacgacgc aaccggagac caaggtc
//
LOCUS       DASUTG010000110         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1796188, whole genome shotgun sequence.
ACCESSION   DASUTG010000110 DASUTG010000000
VERSION     DASUTG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796188"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF089_02445"
     CDS             <1..224
                     /locus_tag="VF089_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172845.1"
                     /translation="LVFCENGSSVDTVIVNGEVVVKSGRLTCMDEQEVLALATKARQR
                     IDPSIQRELAAAKSVEPALSEMCARVFRD"
     gene            complement(370..1404)
                     /locus_tag="VF089_02450"
     CDS             complement(370..1404)
                     /locus_tag="VF089_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6172846.1"
                     /translation="MAKIAGLFIALLLVGAASTDTQAASAEQSFYKGKTIRLIVGFAA
                     GGGFDAYARAIARNLSRHIPGNPTVIVDNMTGAGSRISANYLYKAPPDGLIIGNFIGS
                     LVLQQIMGDKSVEFDGRKFEWLGAPVQDETVCALTKASGINSLDDWFAAKKPVKLGGE
                     APGANDSDAPRILKAALGLPIQLIEGFKGTSNIRIAAEAGEVDGGCWTWASIRTTWSK
                     GLESGLVKPIIQINPKKAADIPNVPNAIDYAKTAEARVLIESGTHAPSAILRAYALAP
                     GTPKERVNTLRSAFNTTMKDPEFVAEMNKSKLEINPLTGAEMESIVKKLFAMDAANVA
                     KIREVLVPKN"
     gene            complement(1452..2507)
                     /locus_tag="VF089_02455"
     CDS             complement(1452..2507)
                     /locus_tag="VF089_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172847.1"
                     /translation="MPPVIDPNNIYSETRADKLSAAVAKHLSRIYVPNRSSNDVWVID
                     PTTDKVVNKFRVGVHPQHVVPSWDLKTLWVNNNAEGRDDGSVTPIDPVTGKPGKNIPV
                     DDPYNMYFTPDGSAAIIVAEARKRLDFRDPQTMAMKYSIETPNCAGINHADFSMDGRY
                     AFFTCEYAGSLVKIDLVEKKVLGYLKFSKGGMPQDIRISPDGRIFYVADMKAAGVFLV
                     EGESFKEVGHIKTGLGAHGLYPSRDGTQLYVANRGVARVYGPPKGKGTVSVIDFATRK
                     VITDWPIPGGGSPDMGNLSVDGNTLWLSGRYDNAVYKIDTTTGQSKIIPVKTEPHGLT
                     VWPQPGRYSLGHTGNLR"
     gene            complement(2752..>3264)
                     /locus_tag="VF089_02460"
     CDS             complement(2752..>3264)
                     /locus_tag="VF089_02460"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172848.1"
                     /translation="IGKDLDLETGHSDHASAFYMYATQAAEVEVDEDSGRVRILRMSA
                     AHDVGKAINPLNCVAQIEGGVVMGIGSALHEEMVIDQTGKVRNPSFLDYHLVTSLDTP
                     KMIPIIVECHEPEGPYGAKGLGEPGLAPTPAAIGNAVADALGVRVYDLPLKPENVYWA
                     IQNKKKKNAA"
BASE COUNT          697 a          872 c          901 g          794 t
ORIGIN      
        1 agctagtttt ttgtgaaaac ggcagttcgg tagataccgt gattgttaat ggtgaggtag
       61 ttgtcaaatc gggtaggcta acctgtatgg acgaacaaga agttctcgct ttagcgacca
      121 aggcccgcca acgaatcgat ccctccattc agcgggagct cgctgcagct aaatccgtgg
      181 agccagcgct gtcagaaatg tgcgcgcgcg tattccgaga ctagctttgt tgagctccgc
      241 tagtccgcta acgcttgcgg catcgccggg attgaaccgt caaactgttg cgaaggcaga
      301 ttgaaaccgt tggagcctca agcgaactga ccctattaag gacgaatact taaggcgcat
      361 tatcacgcgc taatttttgg ggacaaggac ctctctgatt ttcgcgacat tggcagcatc
      421 catggcgaac agtttcttga ctatgctttc catctctgcg cccgtcaatg gattgatctc
      481 cagcttcgac ttgttcatct cagcaacgaa ttccggatct ttcatagtcg tgttgaaagc
      541 gctgcgcaga gtattaaccc gctccttcgg tgttccgggc gcaagcgcat aggcacgtag
      601 gatggcggaa ggagcgtgcg tgcctgattc gatcagcacg cgggcctcgg ctgttttggc
      661 ataatcgatg gcgttgggaa cgttgggaat gtccgccgcc ttcttcgggt tgatctgaat
      721 aattggcttg actagacccg attccagtcc cttgctccag gttgttcgga tcgatgccca
      781 tgtccagcag ccgccgtcaa cttcaccggc ttcggcagcg atgcgtatgt tggaagtccc
      841 cttgaatcct tcgatcagtt ggatcggtag acccagtgcc gctttcaaaa ttctaggcgc
      901 atcggaatcg ttggcgccgg gagcctcgcc cccaagtttg accggcttct tggccgcaaa
      961 ccagtcgtcc aggctattga tgccgctggc tttcgtgagc gcgcagacgg tttcatcctg
     1021 caccggcgcg ccgagccact cgaactttcg tccgtcgaac tcgacgcttt tgtcgcccat
     1081 gatctgctgc aagaccaatg agccgatgaa gttcccaata atcaggccat cgggcggtgc
     1141 tttatagagg taattggccg agattcggct gccagcgccg gtcatgttgt cgacgatgac
     1201 tgtcggatta ccgggaatgt gccgactgag attgcgcgcg atcgcacgcg cgtaggcgtc
     1261 gaatccaccg ccggcggcaa agccgacgat tagccgtatt gtcttgcctt tgtagaaact
     1321 ttgctccgcg ctggcggctt gggtgtcggt gcttgcggcg ccgacaagaa gaagcgctat
     1381 aaagagaccg gcaatttttg ccataaagcc ctccattgag cgaaatgttg ggttaacgcc
     1441 tggcaatagc tttagcgcag atttcccgta tggccaagag aataacgacc cggctgcggc
     1501 cacacggtca aaccgtgagg ctcggtcttg accggaataa ttttcgactg acctgttgtc
     1561 gtatcgattt tgtacaccgc attatcatag cggccggaaa gccatagggt gtttccgtcg
     1621 acgctgagat tacccatgtc tggactgccg ccacccggaa tcggccagtc agtgattact
     1681 ttacgcgtcg cgaaatcgat cacgctcacg gtgcccttac ctttgggagg gccgtagacc
     1741 cgagcgacac cccgattagc gacgtagagt tgcgttccat cgcgactagg atagagaccg
     1801 tgcgcgccca agccggtttt gatatgaccg acctccttaa aactctcacc ttcaaccagg
     1861 aatacgcccg ccgctttcat gtcggcgacg taaaaaattc tgccgtcggg agaaatgcgg
     1921 atatcctgcg gcattccgcc tttggaaaac tttagatacc ctaatacttt cttctcgacc
     1981 agatcgatct tgaccagact accggcgtat tcacaagtga aaaacgcgta acgaccatcc
     2041 atggagaaat cggcatggtt gatgccagcg caattagggg tctctatgga gtacttcatc
     2101 gccatggttt gcgggtcgcg gaaatcgagc cgcttgcgtg cttcggcgac gataatcgcg
     2161 gcgctgccgt ccggtgtgaa gtacatgttg tagggatcat cgactggaat attctttccc
     2221 ggtttgccgg ttactggatc gatcggggta acgctgccgt cgtctcgtcc ttcggcgttg
     2281 ttattgaccc agagagtttt caaatcccag gaaggaacaa cgtgctgcgg atgaacgcca
     2341 acacggaatt tgttgacgac tttatcggtg gttgggtcga tcacccagac gtcgtttgaa
     2401 ctgcgattcg gcacatagat gcgcgatagg tgttttgcaa ccgctgcgct cagcttgtca
     2461 gcgcgcgtct cgctgtaaat attgtttgga tcgatgacgg gaggcattcc cggcacagtt
     2521 tgaatcccgc gagggattcc atctggccgt gattcggccg cgatcgtatg agatccgggg
     2581 aaggggatga gcgcaaaggt tagagcaaat atcgcaaaga gctttttcct ttgggtaccg
     2641 atcacatcga cctccggcag ttatgggtca aagaggcaga gcctctgatg actcccgcct
     2701 cttggaatgc cgtatattaa acgaaaagga aaaaaattac gacgtgagac ttcaggccgc
     2761 gttttttttc ttcttattct ggattgccca gtagacgttc tccggtttga gcggcagatc
     2821 gtagacgcgc actcccaatg catcggcgac agcattgccg atggcggcgg gcgtaggcgc
     2881 aagcccgggt tcccccaacc ctttggcgcc gtaaggccct tcgggttcat ggcattcaac
     2941 aatgatcgga atcatcttcg gagtgtccag tgacgtaacc aagtgataat cgaggaaaga
     3001 aggattgcga acctttccgg tttgatcgat gaccatctcc tcgtgcagcg ccgagccgat
     3061 gcccatgacc acaccgcctt cgatttgtgc gacgcagttc agcgggttga tcgctttgcc
     3121 gacatcgtgg gcggcggaca ttcgcagaat tcttacccga ccgctatcct catctacttc
     3181 tacttccgca gcttgcgtgg cgtacatgta aaaagccgat gcgtgatcgc tgtgaccggt
     3241 ttcgagatcg agatccttgc cgat
//
LOCUS       DASUTG010000111         7284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_610249, whole genome shotgun sequence.
ACCESSION   DASUTG010000111 DASUTG010000000
VERSION     DASUTG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610249"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VF089_02465"
     CDS             <1..999
                     /locus_tag="VF089_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEX6172849.1"
                     /translation="HEFALDFAKKVNDTTGGDLKIEVLPAGAVVPAFQLLDAVSKGTL
                     DGGHGVVVYHYGKQTALALWGSGPSYGMDANMMLAWHKYGGGKDLLEKLYKSIGANVV
                     SFLYGPMPTQPLGWFKKPITRADDIKGLKFRTVGLSIDVFTGLGAAVNALPGGEIVPA
                     MDRGLLDAAEFNNASSDRLLGFPDVSKVCMLQSYHQNAEQFEILFNKTKFDALPAKMK
                     ALIESTVEAASQDMSWKAIDRYSKDYVEMQAKDKVRFYRTPDSVLQRQLQTYDAAADK
                     KAAENPMFKEIAASQRLFAARAVKWDLDTNVGRRMAYNHYFAKAAGKTAPEASKKS"
     gene            1051..1749
                     /locus_tag="VF089_02470"
     CDS             1051..1749
                     /locus_tag="VF089_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX6172850.1"
                     /translation="MAGVLYTVNAYRLLRAIDQLSYWSGKAFAWLIVALTLVVSVEVF
                     KRYILNAPTAWIFDFNNMLYGTLFMMCGAYTLSLSGHVRADFIYARLSPKMQAALDLA
                     LYILFFVPGILALIYAGYGYAAESWQIGEHSTVTAEGPPVYHFKSVIPIAGALVMLQG
                     VAEIARCILCLRTGTWPPRLEDVEEIDVVGGQLAHSEFVDEESRRAAAGSAHALDEAA
                     RQRTLVDERKGRQR"
     gene            1746..3785
                     /locus_tag="VF089_02475"
     CDS             1746..3785
                     /locus_tag="VF089_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6172851.1"
                     /translation="MSDPALGLTMLGLIVIAIMMGFPTAFTLMGLGMLFGFLAFWTPS
                     QHWYDNQVFDLMVQRTYGVMTNDTLLSVPLFVFMGYIMERAALVDRMFHSVQLAFRRV
                     PASLAVTTLLVCAFWGIASGIVGAVVVLMGVIAMRPMLNAGYDVRLASGAITAGGTLG
                     ILIPPSVMLIVYAAVAGESIVKLYAAAMLPGFFLTFLYLAFILGWAIINPKIAPKLPP
                     EQYRIATPEWLRRLERGSARRIVAGLLGAMFRPKLLQGAMSPDGHPITYGLIVKNVLT
                     LMVPAVLTVGLFATAWWYVVIYNAPSATVAPEAGGPQQLALPPAALEEKPQEASEEKL
                     QELGGGSMAESSESPLPPDGPPQEMGSLAEQPIASATGKTPEYFYLGFWGLAAFSVLP
                     LAMYYWRMDGEQLEILKELVVSVVPLAVLTAVVLAVILFGITTATESAAVGALGALYL
                     AVMAKYPRQVWTWTLVGAVIGVALSWQRGGVVTVLVSGSLGGVFAGTLVPWFWGLGRS
                     KELWQNLKESVFLTAKTTAMVCWLFIGSALFSGVFALHGGQALIESWVLGMNLSPVGF
                     LIMAQLIIFLLGWPLEWTEIIVIFCPIFIPMLGHFNVDPILFGTMVAVNLQAAFLSPP
                     VAMSAFYLKGVAPSHVTLNQIFAGMMPYMLIVVLCLICMYIWPGMTLWLPEFLYG"
     gene            4097..5404
                     /locus_tag="VF089_02480"
     CDS             4097..5404
                     /locus_tag="VF089_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6172852.1"
                     /translation="MIRDGIISSEQLVEACLVRIREVDPRIQAWTFLDADYALAQARA
                     LDELRLSGRAIGPLHGIPVSIKDIFDTADMPTECGSVLYSGRTPSRDATAVSRLRAAG
                     AVIMGKTVTTEFAYFGPGKTRNPHNPEHTPGGSSSGSAAAVAASMVPLALGSQTNGST
                     IRPAAYCGVIGFKPTQGLISRHGALTLSRTLDHVGLFANSVQDIALLAEEIVGYDEND
                     PDTRPRARVPFAGIAAEEPPLAPMFAFIKTPHWENVDEDTREGFAEIIDALGNQVEEI
                     ELFPSAGDAWQWHQCIMSAEMAYNLEREWRTGGTRLSQQLRAQIERGREVRAADYLSA
                     LARIKLVNDSFIELFEQRYDAILTPAAPSAAPKGLDSTGDPSFCTLWTLCGMPALSLP
                     LLQSSAGMPLGVQLVGPHYGDARLLRTARWLATQVAAEREDKS"
     gene            complement(5575..6423)
                     /gene="lipA"
                     /locus_tag="VF089_02485"
     CDS             complement(5575..6423)
                     /gene="lipA"
                     /locus_tag="VF089_02485"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6172853.1"
                     /translation="MPRRHPEWIKVKAPGSPNYLRLKRILREKNLHTVCEEARCPNIG
                     ECWGNKTATFLILGDTCTRGCRFCAIDKGKPLALDPEEPRNVALVVKDLGLDHIVVTS
                     VNRDDLPDGGSSHFVKTVFWIKSLSPGIRVELLIPDFDGNLAAVETVVNSGIEILNHN
                     IETVPRLYGKVRPGHSYGCSLNILKRAKEMRPEVLTKSGFMLGVGESYGEVIATLHDL
                     RRNQVDIVTLGQYLQPSNRQLKVERYVTPAEFSDFKIAAEKLGFRHVESGPLVRSSYH
                     AWSHVN"
     gene            complement(6509..7207)
                     /gene="lipB"
                     /locus_tag="VF089_02490"
     CDS             complement(6509..7207)
                     /gene="lipB"
                     /locus_tag="VF089_02490"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6172854.1"
                     /translation="MGRLLYQDLGRLDFVAALSRQEQLIELKRQGIADILLFVEHPHV
                     YTIGRGGDLNNVLAARDVPLYRTSRGGDVTYHGPGQLVVYPIIDLRSKLRRDVHRYVR
                     NLEITAIKTLNDFSLKAKRRPPYTGIWIDSRKIAAIGIAVRRGITFHGLALNVNTDLS
                     YFDRIIPCGLTWADVTSMAKELGKDPSVHNVRESFLCHFAELFGYTEIENISHFSEFQ
                     AGQNGARYEARDDR"
BASE COUNT         1459 a         2014 c         2105 g         1706 t
ORIGIN      
        1 cacgagtttg ccctcgactt cgccaagaag gtcaacgaca cgaccggcgg cgatttgaag
       61 atcgaggtgt tgcctgcggg cgcggtagtg ccggcgttcc agcttctcga cgcggtgtcg
      121 aaaggcacac tcgacggcgg ccatggcgtg gtcgtgtacc attatggcaa gcagacggct
      181 ctggcgcttt ggggctcggg tccgagctac gggatggatg ccaacatgat gcttgcctgg
      241 cacaagtacg gtggcggcaa ggacttgctc gaaaagctct acaagtccat cggcgccaac
      301 gtggtctctt ttctgtacgg cccgatgccg acgcagccgc ttggctggtt caagaagccg
      361 atcactaggg ccgacgatat taaggggctc aaattccgca ccgtcggtct ctcgattgac
      421 gtgttcacgg gcttgggcgc ggcggtgaac gcgctcccgg gcggcgagat cgtgccggcg
      481 atggaccggg gactgctcga tgccgccgag ttcaacaatg cgtcctcgga tcgtctcctt
      541 ggcttccctg acgtttccaa agtttgcatg ttgcagagct accaccagaa cgctgagcag
      601 tttgaaattc tgttcaacaa gacgaagttc gatgcgctgc cggcgaagat gaaggcgctt
      661 atcgaatcga cggtggaagc cgcttcgcag gatatgtcgt ggaaggcgat cgatcgctac
      721 tcaaaggact acgtcgagat gcaggccaaa gacaaagtca gattctacag gacgccggac
      781 tccgtgctcc agaggcaact ccagacatac gacgccgcag ccgataagaa ggccgccgaa
      841 aacccaatgt tcaaggagat tgctgcgtca cagcggctct ttgctgcgcg ggcggtgaag
      901 tgggatctgg acactaacgt tggccgccgc atggcctaca accactattt cgccaaagcg
      961 gcaggcaaaa ccgccccaga agcaagcaag aagagctaat ttgtccgtgg accggatccc
     1021 gcttcaacac cagccatcag tcccaggttg gtggctggtg ttttgtacac cgtgaatgcc
     1081 taccggctac tccgagccat cgatcaactc agctactggt cgggcaaggc cttcgcatgg
     1141 ctgattgtcg cgctcacatt ggttgtctcg gtggaggtct tcaagcgcta catccttaat
     1201 gctcccacag cctggatctt cgacttcaac aacatgctct acggcacgct gttcatgatg
     1261 tgcggcgctt acaccttgtc gctcagtggg cacgtgcgcg ccgactttat atacgcccgg
     1321 ctgtcgccga agatgcaggc ggcgctggac ttagcactgt atatactttt ttttgtccca
     1381 ggcatcctgg cacttatcta tgccggctac ggttacgctg ccgagtcgtg gcagatcgga
     1441 gagcactcga ctgtgaccgc ggagggtcca ccggtttatc acttcaagtc cgtgatcccg
     1501 atcgccggcg cgctggtgat gctccagggt gtcgcagaga tcgcgcgttg catcctgtgc
     1561 ttgcgcaccg gcacctggcc gccgcggttg gaagatgtag aggaaataga cgtcgtcggc
     1621 ggacagctcg ctcacagcga gtttgtcgac gaagagtcgc gccgcgctgc ggccggatcg
     1681 gcacacgcgc tggacgaagc cgcgcgccag cgcaccctgg tggatgaaag aaagggaagg
     1741 caacggtgag tgatccagcg ctcggattga ccatgcttgg gctcatcgtg atcgctatta
     1801 tgatgggctt tccaaccgcc tttaccctga tggggctggg gatgcttttt ggctttctcg
     1861 ctttttggac tccttcacag cattggtacg acaatcaggt cttcgatctc atggtgcagc
     1921 gcacgtatgg tgtgatgacc aacgatactt tgctgtctgt gccgctgttc gttttcatgg
     1981 gctacattat ggagcgcgcc gcgctggtcg accgaatgtt tcatagcgtc cagcttgcct
     2041 ttcggcgagt gcccgcgtcg ctcgccgtga ccactttgct ggtttgcgcc ttttggggaa
     2101 tcgcctcggg catcgtcggc gcagtggtgg ttctgatggg agtgatcgct atgcgtccga
     2161 tgctgaatgc cggttacgac gtacggctcg cgtcgggtgc aatcaccgcg ggcgggacgc
     2221 tgggtatttt gattccgccc tccgtgatgc tcattgtcta tgcggcggtg gcgggagagt
     2281 cgatcgtgaa gctctatgcg gcggcaatgc tgcccgggtt tttcctaacg ttcctctatc
     2341 tcgccttcat tctcggctgg gcaatcatca atccgaagat tgcgcctaag ttgccgccgg
     2401 aacagtatcg tattgcgacg ccggaatggc tccgacggct cgaacgtggt tccgcccgac
     2461 ggatcgttgc cggtctcctc ggggccatgt ttcgaccgaa attgttgcaa ggcgcgatgt
     2521 cacccgacgg tcatccgata acttatgggc tgatcgtcaa gaacgtcctc acgctgatgg
     2581 ttccggcagt actgacggtg ggattgtttg ccaccgcctg gtggtatgtt gtcatctata
     2641 atgcccccag cgccacggtc gctccggagg cggggggtcc gcagcagctg gccttaccgc
     2701 cagctgcttt ggaggaaaag ccgcaggaag cgtccgagga gaagctgcaa gagctcgggg
     2761 gcggaagcat ggcggagtcc tctgaatcac ctctgccccc tgatgggccg cctcaggaaa
     2821 tgggctctct ggccgagcag ccaatcgcat cggcgacggg caaaactccg gaatactttt
     2881 atctcgggtt ctggggactg gccgcgttct cggtccttcc tcttgccatg tactactggc
     2941 gcatggatgg cgagcagctc gaaatattga aggaactagt cgtctcggtc gtgccgctcg
     3001 ccgtgctgac ggcggtggtg ctcgcagtga ttttgtttgg catcactacg gcgaccgagt
     3061 cggctgcagt gggagcgctc ggcgctctct atctcgcagt gatggccaaa tatccgcgcc
     3121 aggtttggac gtggactctg gtgggagcag ttattggcgt ggccctaagc tggcagcgcg
     3181 gcggcgtagt gactgtgctc gtatccgggt ctcttggagg tgtattcgcg ggaacgttag
     3241 ttccttggtt ttggggtctg ggaaggtcaa aggagctctg gcaaaatctc aaagaatcag
     3301 tttttctgac tgcgaagacc acggctatgg tttgctggct cttcatcggg tccgcgcttt
     3361 tctccggtgt ctttgcgctt catggtggac aggcgctcat cgagagctgg gttctgggga
     3421 tgaacctttc tcccgtcggc ttcttgatca tggcgcagct gatcatattt ctacttggct
     3481 ggccgctcga atggaccgaa atcatcgtga tcttctgtcc gatattcatc ccaatgttag
     3541 gccatttcaa tgtcgatccg attttgttcg gtacgatggt agcggtgaac ctgcaagcgg
     3601 cgtttttgtc gccgcctgtt gcgatgtcag ctttttatct gaagggtgtg gcgccgtcgc
     3661 atgtgacgct caatcaaatt tttgccggta tgatgcccta catgctgatc gtggttctct
     3721 gtttgatctg tatgtatatc tggcccggga tgacgttgtg gctgcccgaa ttcctctacg
     3781 gttagtgccg tgacgataag cttcgcattc ctgcgttctc ggtcgtcgat catcctcaac
     3841 gtactgacga cgtacgtttc cggtgccctt ctccctcgag ccttggacta ttcgcttctc
     3901 gccacggcac acatgcatgg tcaaggattt tatgacaaca tccgatgccg gctttagaga
     3961 tgaaggctgg aaccgagaaa agcgagaagt tttgtctcac attttctaat tcaatttggc
     4021 caggacatct ggtttgtaat tacgctggat ggaactaacc gatcttcact tgctctcggc
     4081 ttcggaggca gccgggttga tccgggatgg cattatcagc tcggaacaat tagtcgaggc
     4141 ttgcctcgtg cgtattcggg aggtggaccc gagaatacaa gcctggacgt ttctcgatgc
     4201 ggactatgct ctggcgcagg cgcgcgcgct ggatgagttg cggttatcgg ggcgggcaat
     4261 cggtccgctg cacggcattc ctgtcagcat caaagatatt ttcgataccg ccgacatgcc
     4321 tacggagtgc ggttcggtgc tctactccgg acggacaccc tcgcgcgatg ccacggccgt
     4381 atcacggctc cgtgcggccg gcgccgtaat catgggcaaa accgtaacca ccgaattcgc
     4441 ttatttcggg cccggaaaga cacgcaaccc gcacaatcct gagcacaccc caggcggctc
     4501 gtccagcggt tcagccgctg cggtggcggc cagcatggtg ccgctggccc tcggaagcca
     4561 aacgaacggt tcgaccattc gtccggcggc gtattgcggt gtcatcggat tcaagccgac
     4621 tcaaggtctg atttcgcgcc acggcgcctt gacgctgtcg cgcaccctcg atcatgtcgg
     4681 cctgttcgcg aattccgttc aagatatcgc tctgctcgcc gaagaaatcg tcggctacga
     4741 cgagaacgat cccgacacgc ggccacgggc gcgtgttccg ttcgccggga tagccgcgga
     4801 agagccgccg ttggcgccga tgttcgcatt catcaaaact cctcactggg aaaacgtcga
     4861 cgaagatacc cgtgagggat tcgccgaaat tattgatgct ctcggcaatc aggtcgaaga
     4921 aatagagttg tttccttcgg cgggggacgc ctggcagtgg caccagtgta ttatgagcgc
     4981 tgaaatggcg tacaacctgg aacgcgaatg gaggaccggc ggcactcgtc tatcgcaaca
     5041 actgcgggca cagatcgagc gcggacgcga agtgcgcgcg gccgattatc tcagcgcgct
     5101 cgcgcgaata aagttggtta atgatagttt tatcgagctc ttcgaacagc gctacgacgc
     5161 aattctcact cctgccgcac ccagcgccgc accgaaaggt cttgacagca ccggcgatcc
     5221 atcgttctgc acgctctgga ctctttgcgg catgcctgcg ctgagcttgc cgttattgca
     5281 gagcagtgca ggaatgcctc taggcgttca actcgtcggt cctcattacg gtgacgcgcg
     5341 gctgttgcgc acagcgcggt ggttggccac gcaggtggcg gcagaaagag aggataagag
     5401 ctgaaagcgc cagagatgaa gatcagctac tgatcagtcc tgatccctat tctatctcgg
     5461 caagaacatc aagatcggaa cggctaacgc aaacgggcgc tactgaaccc gaggctcgag
     5521 aactaaactt cacaacacta atactcagac gcggttcagg tctgccttcg cctcctagtt
     5581 cacgtgactc cacgcatggt acgaactgcg caccagcgga cccgattcaa catgacgaaa
     5641 accgagtttt tcggcggcaa tcttaaaatc gctgaattcc gccggcgtga catagcgctc
     5701 tactttgagc tggcggttgg agggctgcag gtattgccct aaagtaacga tatcgacttg
     5761 gttccgacgc aggtcgtgca gtgtcgcgat gacctctcca taactctcgc cgacgccaag
     5821 cataaagccc gacttggtca atacctccgg gcgcatctct ttggcccttt ttaaaatatt
     5881 taatgaacat ccatagctgt ggccgggtcg aaccttgcca taaaggcgcg gcacggtttc
     5941 gatgttgtgg ttgagaattt caatcccgga gttgaccacc gtttcgacag cagccagatt
     6001 accgtcgaag tcaggaatca gcagttcgac gcgaatgcca gggctgaggc tcttgatcca
     6061 aaaaaccgtt tttacgaaat ggctcgagcc accgtccggc agatcatcgc gattcacgga
     6121 agtcaccacg atgtgatcca agccgaggtc tttaacgacc agcgccacat tccgcggttc
     6181 ctcgggatcg agcgccagcg gtttaccttt atcgatcgcg cagaaccggc aaccgcgcgt
     6241 gcaggtgtct cctagtatca gaaacgtcgc ggtcttattg ccccagcatt cgccgatatt
     6301 cgggcagcgc gcttcctcac aaaccgtatg aagatttttt tcgcgcagaa tacgtttgag
     6361 acgcagatag ttgggacttc ccggggcttt gactttgatc cattctggat gtcttcgcgg
     6421 cattatcttt taccagtctg gtggaaaagg aaatctactt cgttgcactg tccgattcgc
     6481 tccaacgtaa taacacgtac gcctccgctc atcgatcatc acgcgcctcg tacctggcgc
     6541 cgttttgacc agcctgaaac tctgaaaaat gagaaatatt ttcgatttcc gtatagccaa
     6601 acagctcggc gaagtggcaa agaaaacttt ctcttacgtt atgtacgctt ggatctttgc
     6661 ccagttcctt ggccatcgac gtcacatccg cccaagtgag cccgcacggg atgatgcgat
     6721 cgaaatagct aagatcagtg ttcacgttca gcgctaaacc gtggaaagta atgccccggc
     6781 gcaccgcgat gcctatagcg gcgatttttc gactatcaat ccagatgcca gtatacggcg
     6841 gtcggcgctt ggctttgagg ctaaagtcat tgagtgtttt gatcgcagtt atctcgaggt
     6901 ttctgacata gcgatgcacg tctctacgca atttggagcg caggtcgatg atcggataga
     6961 ccactagttg acccggtcca tgataagtga catcgccgcc gcgactggtg cgatatagcg
     7021 gcacgtctcg cgcggcgaga acattattga ggtcgccgcc gcggccgatg gtatagacat
     7081 gcggatgttc gacgaagagg agaatatcgg caattccttg acgctttagc tcgatcagtt
     7141 gttcttgccg tgacagtgct gcgacgaagt ccaaccgccc aaggtcttgg taaagcaatc
     7201 ttcccattag ttgtttttag tcttacccac cacggccgga tctttcaagt tagtttagtg
     7261 cggaggcata gtagaacgtt tgac
//
LOCUS       DASUTG010000112         7416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_461742, whole genome shotgun sequence.
ACCESSION   DASUTG010000112 DASUTG010000000
VERSION     DASUTG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461742"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VF089_02495"
     CDS             <1..1436
                     /locus_tag="VF089_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6172855.1"
                     /translation="KVARLFSLYGRELLVILASDLEIYNDREMSGYGLNFTWRSRDSR
                     TNTERAIVYFSKEKVRAFLRQDINDNTLLGDAVIFVIEPEGQANLLSFRAQHPAPDVR
                     APIKEQVLLPEPAKEEAELKPLLAERSVSSMEPIKPASNAGESSAGEKDNWMASSKSE
                     TAPKPITPNLSLKIQEEIVPDEKIGPVVSGNTEPVGKASSRESGDQTDKSSTPARGIV
                     ETAQPAPRAVKSSQPQLGAEAIQSKLPHKNSERKIPELSSLVEVGKEKEQKEVHLDLP
                     DPQPPVLREGSWPNEASIPELGIRALPAPGSTEVSLSSAKTPNIGGKVKRVSDIASIT
                     KITPDTAPTETAVPAPNTPSGLQNPEGDNVERHSVLAHAKPMENIPDRSFVRPLPKSL
                     EGYIIQVPFKDRSEARSWADTFEQRGYAVSITEASGGESLRVRIGNFQLRDDAERQLK
                     SIREDGIMGIILNLPQAYRPEVRSSLP"
     gene            complement(1460..2923)
                     /gene="gatB"
                     /locus_tag="VF089_02500"
     CDS             complement(1460..2923)
                     /gene="gatB"
                     /locus_tag="VF089_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX6172856.1"
                     /translation="MKYEIIIGLEVHAQLLTDSKIFCGCSTRFGATPNHNTCPICAGF
                     PGALPVLNKKVVEFALQAGIAMNCKIAGYSRMARKNYFYPDLPKGYQISQYELPICTN
                     GYIDINVDGSTRRVRLTRIHMEEDAGKNIHDAYDDSSLIDLNRAGVPLLEIVSEPDIR
                     NATEAVTYLKSLRSILRYLEVCDGNMEEGSFRCDANISVRTTGSEALGTKVEIKNLNS
                     FKAIEKSLQYETERQIDTLSEGGTLSQETRLWDDNNEQTRSMRSKESAHDYRYFPDPD
                     LLPINVDEAWIQKLRSTLPELPEARKVRFMSQYGLPAYDAELLTSRRDVADYLEAAIT
                     VCPNAKAVSNWIMGDLFRLLKERKLDERLRITDWPLQPAQLGGLVLLIDQGKISGKIA
                     KIIFDTLLDTNQSPEVIITERGLEQVSDLASLERSIDQVLTNHAKQVAAYHNGNDKVF
                     GFLVGQVMKALQNKANPQKVNELLKAKLSSLSRTDAV"
     gene            complement(2920..4386)
                     /gene="gatA"
                     /locus_tag="VF089_02505"
     CDS             complement(2920..4386)
                     /gene="gatA"
                     /locus_tag="VF089_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX6172857.1"
                     /translation="MNLFSLTLHEASEKLRKREVSSQELTEAVFRRVAQTDGILRSYI
                     TVCRDAAIAQAKEADNRLRQGPAASPLLGIPIALKDNLLTRGLRTTCASRILDNFIPP
                     YDATAVAKVRSAGAVITGKTNLDEFAMGSSAENSAFFSTRNPWHLERVPGGSSGGSAA
                     SVAADQCIAALGTDTGGSIRQPAAFCGIVGVKPTYGRVSRYGVIAFASSMDQVGTLTK
                     DVRDSALLLEAISGHDPADSTSVKLPVPSYSKSLDGDIAGLRLGIPKEYFTAGLQPAV
                     DQAVVLAIRQLENRGAIVEEVSLPHTEYAVAVYYIIAPAEASSNLARYDGMRYGQRAA
                     GRDLTETYMLTRAQGLGPEVKRRIMLGTYALSAGYYDAYYLKAQKVRTLIQRDFDAAF
                     KRCDAIITPTSPTTAFKISEKIQNPLQMYLSDICTISSNLAGVPAISVPCGLDREGLP
                     IGMQVIGKHFDEATILRVAYAYEQETEWHRKKPYISEA"
     gene            complement(4383..4667)
                     /gene="gatC"
                     /locus_tag="VF089_02510"
     CDS             complement(4383..4667)
                     /gene="gatC"
                     /locus_tag="VF089_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX6172858.1"
                     /translation="MLTHEDVAQIALLARLRLLPEEVNQLTAELQSILQHMEKLQELD
                     TSEVEPFTQAVDTSNAFREDVVTIIPDPDGILANAPAKVKTFFKVPRIIE"
     gene            4843..5568
                     /locus_tag="VF089_02515"
     CDS             4843..5568
                     /locus_tag="VF089_02515"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6172859.1"
                     /translation="MGGNQFTLSKDFDRPVRQGTIERHSYSRGFKCVAGLDEVGRGPL
                     AGPVVAAAVVLPRGFKHSDIKDSKMLTAEQRERLLPTIKKSALCWGVGVVEVEEINRI
                     NILQASFLAMIKAFRALWPVPDCLLVDGNRKIPAEFFQMNPVFQQLPLQKTIIKGDQL
                     CVSIAAASILAKVARDTMMVELDKTYPEYGFAHHKGYSCLMHMEALRRLGPSPIHRRS
                     FKPVRAMCALDLETHLDLPFVNP"
     gene            5565..5936
                     /locus_tag="VF089_02520"
     CDS             5565..5936
                     /locus_tag="VF089_02520"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX6172860.1"
                     /translation="MILNKARIILGKQGERLAELFLKKKGYKLVERNYRCAAGELDLI
                     MLDQRVIVFVEVKTRTGIRYGTPLEAVEVRKQQKMICAAQFFVTAKKLNQREARFDVV
                     GISWRGAQPHVEHIENAFELV"
     gene            5946..7232
                     /gene="serS"
                     /locus_tag="VF089_02525"
     CDS             5946..7232
                     /gene="serS"
                     /locus_tag="VF089_02525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6172861.1"
                     /translation="MLDIRLLRDDLEQVKTRMATRGVKIDWGEFLSLDRQRRDALSRL
                     EKLKEQKNRLSGEIGNLKKSGADATGLMREVEQLSQQIRESERPLAEVEERFEQFMLT
                     VPNLPHINVKVGKDSDDNREIRRWGAPPKFDFHARNHWEIGEELDILDFQRAAKLAGA
                     RFVIYKGAAARLERALINFMLDLHTGEHGYKEIIPPALANRAALTGTGNLPKFEEDLF
                     QIESPEYFLIPTAEVPLTNVHREEVLQRESLPIKYTAVTMCFRREAGSYGKDVRGLIR
                     QHQFNKVEMVKLCEPEFSYGELETMVKDAEEVLKRLELAYRVVELCTGDMGFASAKTY
                     DIEVWLPGQDTYREISSCSNCEDFQARRAQIRYRKENGKPNFVHTLNGSGLAVGRTLV
                     AILENYQQKDGSVLIPTALRPYMGGLEKIVRDGRLE"
BASE COUNT         1819 a         1811 c         1990 g         1796 t
ORIGIN      
        1 tgaaagtagc gcggttgttc tctctttatg gcagagagtt gctagtaatc cttgccagtg
       61 atctcgaaat ctataacgat agagagatgt cgggttacgg cttgaacttt acctggcgaa
      121 gtcgggattc ccgcacaaac acggagcgcg cgatcgtcta tttttccaaa gagaaagtaa
      181 gggcattttt aagacaggat ataaatgaca atacgctcct cggtgacgcg gtcattttcg
      241 tgattgagcc ggaggggcag gcgaaccttt tgtctttccg tgcgcaacac cccgcgcccg
      301 acgttcgggc gccgattaag gaacaagttc tcttgccgga gcccgccaaa gaagaagcgg
      361 agctcaaacc actgttggca gaaaggtccg tcagcagtat ggaacctatc aagccggcga
      421 gtaacgccgg ggagagttca gccggtgaga aggacaattg gatggcaagt tctaaaagcg
      481 aaactgcgcc gaaaccgata actcccaatc tgagccttaa gatacaggaa gagatcgtcc
      541 cagacgaaaa gatcggtccg gttgtgagcg gaaacaccga gccggttgga aaggcttctt
      601 cgagagaatc aggagaccaa actgataaga gtagcacgcc ggctagggga atcgttgaaa
      661 cagcgcaacc tgccccacgt gccgtgaagt cctcccagcc gcaactcgga gccgaagcga
      721 tacagtcgaa gctgccgcat aagaacagcg aacggaaaat ccctgaattg agcagcttgg
      781 ttgaagtagg taaggaaaaa gaacaaaaag aagtgcatct agacctgccc gatcctcagc
      841 ccccggtgct cagggaagga tcgtggccga acgaagcgtc aataccggaa ctgggaatac
      901 gtgcgcttcc tgcaccggga tcgactgaag tgtcgttgtc ttcagctaaa acgccaaaca
      961 ttggcggtaa ggtcaagagg gtaagcgaca tagcatcgat aacgaagata actccggata
     1021 ccgcacctac cgagacggcg gtgccggccc cgaacacacc gtcagggctg cagaaccccg
     1081 agggcgacaa tgttgaacgg cactctgtcc ttgctcatgc taagcctatg gaaaacatac
     1141 cggacagatc tttcgttaga cctttgccca aatctctgga gggctacatc attcaagtac
     1201 ccttcaagga tcgctctgaa gcgcgaagtt gggcagacac ttttgagcag cgcggctatg
     1261 ctgtctccat aacggaagct agcggaggcg aatcattacg ggtacgtatt ggaaactttc
     1321 aattgcgaga cgacgctgaa cggcaactta aaagcattcg agaggatgga atcatgggaa
     1381 tcattctcaa tcttccgcag gcttatcggc ccgaggttcg ttcttctctg ccgtgatttt
     1441 ttgaacctat ggtctagctt tagacagcgt cggttcggct caatgatgat agtttcgcct
     1501 ttagtaattc attgaccttt tgcggatttg ccttattctg taaagctttc atgacttgcc
     1561 ccacaagaaa gccgaagact ttatcattac cgttgtgata tgcggcgact tgtttggcgt
     1621 ggttcgtcag aacttggtcg attgaccttt cgaggcttgc gagatctgag acctgctcca
     1681 agcctcgttc ggttatgatg acttccgggg attggttcgt atcgagcaag gtatcgaaga
     1741 ttattttcgc gattttcccg ctgattttgc cttgatcgat gagtagcacc aacccgccca
     1801 attgtgctgg ctggagcggc cagtcggtga tccgaagtcg ttcatcgagc tttcgttctt
     1861 ttaaaaggcg gaacagatca cccattatcc aattgctcac ggctttggca ttcggacaaa
     1921 cggtgatcgc tgcctccaaa tagtcggcaa catctctgcg gctggtgagc agctcggcgt
     1981 cgtacgctgg gaggccatac tgcgacatga aacgtacttt tcgcgcctcg ggaagctcag
     2041 gcaaagtgct acggagtttt tgaatccatg cttcatctac attgatcggt aaaagatcag
     2101 ggtccgggaa atatcggtaa tcgtgagccg attccttaga tcgcatcgag cgggtttgct
     2161 cgttattgtc gtcccacagc cgcgtttctt ggctgagagt tccaccttcc gaaagcgtat
     2221 caatctggcg ttctgtctca tactgtaggg acttctcgat agctttaaag gagttcaggt
     2281 ttttgatttc caccttggtt cccaaggctt cagatccagt cgtacgtacc gaaatgttgg
     2341 cgtcacaacg aaagctgcct tcctccatgt tcccatcaca aacttcaaga tacctaagaa
     2401 ttgaacgtaa gcttttcaga taggttaccg cttcggtagc attgcggata tcgggttcac
     2461 tgacaatctc cagcagcggc actccagcac ggtttaaatc gattaaactc gagtcatcgt
     2521 atgcatcgtg aatgtttttt ccggcatctt cctccatatg gatccgggtc aaacgaacgc
     2581 gtctcgtaga gccatccacg ttaatatcga tgtacccgtt ggtgcagatc ggaagctcgt
     2641 actggctaat ctgataccct ttcggcaaat caggatagaa atagtttttt cttgccatgc
     2701 ggctgtagcc ggcgatttta cagttcattg cgatcccggc ctgcaaggca aattctacaa
     2761 ccttcttgtt tagaaccggc aaagccccag gaaatccagc gcagatgggg caagtattgt
     2821 ggtttggcgt cgcaccgaac ctggtggagc agccgcaaaa gattttcgag tcggtcagaa
     2881 gttgagcatg aacttcaagt ccgataatga tttcgtattt catgcctcgg atatataggg
     2941 tttcttccta tgccactctg tttcttgttc gtaagcgtaa gcgacgcgca agatggtcgc
     3001 ttcgtcaaaa tgttttccaa taacctgcat tccgatcggc aatccttcac ggtcgagacc
     3061 gcagggtacc gagatcgcgg gcacaccggc gagattgctc gaaatcgtgc agatatccga
     3121 caaatacatt tgaagtggat tttgtatttt ctcgctaatc ttgaatgcgg tggtcggcga
     3181 ggtgggcgtg ataatcgcgt cgcaacgttt aaatgccgcg tcaaaatccc gctgaatcaa
     3241 ggtacgaacc ttttgtgcct tcaaatagta cgcatcgtaa tatccggctg atagtgcata
     3301 cgtcccaagc atgatacgtc gcttcacctc tggtccgagt ccttgtgcgc gagtgagcat
     3361 atacgtttcg gttaaatctc tgcccgcagc gcgctgacca tagcgcattc catcatagcg
     3421 ggcgagattt gagctggcct cagcaggtgc aatgatgtag tacacagcca cggcgtactc
     3481 ggtatgtggc aatgagactt cttcgacgat ggcgccgcgg ttttcaagct gacgaatggc
     3541 gagcacaacg gcttgatcga cggccggctg caatcctgcg gtgaaatatt ccttgggaat
     3601 acccaagcgt aagccggcaa tatcgccatc tagcgattta gagtacgacg gcactggaag
     3661 cttcacggaa gttgaatccg ctgggtcgtg cccgcttatg gcttcgagca gcagggcact
     3721 gtcacgaacg tccttcgtca gcgtacctac ttggtccatg gaagaagcaa aggcgataac
     3781 cccatagcgg ctaacccgtc cataagttgg tttcacccca actatgccac aaaaagccgc
     3841 cggttgacga atcgagccgc cggtgtcggt tcctaaggct gctatgcatt gatcagcggc
     3901 gacggatgcc gcggatccac ctgaagatcc cccgggtacg cgttccaagt gccagggatt
     3961 gcgggttgag aagaacgctg agttttcagc agatgaaccc atcgcgaact catccaggtt
     4021 agtcttcccg gtaattaccg cgccggccga acggactttc gctacggcag tagcgtcata
     4081 aggcggaatg aagttgtcca atatccttga cgcgcaggtt gtcctcaatc ctctggtaag
     4141 tagattatct ttaagggcga tgggtattcc gagtaaagga gaagcggctg gtccttgcct
     4201 gaggcgatta tcggcttctt ttgcctgtgc gatcgccgca tctcggcaaa cggtaatgta
     4261 ggaccggaga ataccatccg tttgcgcaac gcgtcggaaa accgcctcag tcagctcttg
     4321 cgacgaaact tcgcgcttac gcagtttttc gcttgcctca tggagagtca gtgagaatag
     4381 attcattcga tgattcttgg aactttaaaa aaagtcttca ccttggctgg cgcattcgcc
     4441 aatattccat ctgggtcagg gatgatggta accacatcct ctcgaaatgc attggatgtg
     4501 tctaccgcct gggtaaaagg ctcaacttca gaggtatcca gctcctggag cttttccatg
     4561 tgctgcaaaa tactctgaag ctcggcagtt agctgattta cttcctcggg cagcaagcgc
     4621 aagcgggcca atagcgctat ctgcgccacg tcttcgtgcg taagcatcga gattttctaa
     4681 cacatggaaa ctgtcaattc caatatacaa atcgtagatg atgtggcatc ccgtgctttt
     4741 ggaccgccgt ccgcttcgac gttgtatgtg ttgcggttga gctaatcgtt tccactaaat
     4801 cttatttgca gttgttttct cttttggcta aaagggaaat gaatgggcgg caaccaattt
     4861 acattgtcga aagacttcga ccggcccgta aggcagggca ccatcgaacg acattcgtac
     4921 tcacgtgggt ttaagtgtgt cgccggacta gatgaagttg gccgaggccc tttagcaggc
     4981 ccggtagtag ccgcagcagt cgtgctgccg cgaggtttca agcactccga tatcaaagat
     5041 tcgaaaatgt taacggctga gcagcgggaa agattgctac cgacgatcaa aaagtcagct
     5101 ttatgctggg gcgtaggtgt tgttgaggtt gaggagatca atcgaatcaa tattctccag
     5161 gcgagtttcc ttgcaatgat caaggcattt cgagccttgt ggccggtccc cgattgtttg
     5221 ctcgttgacg gcaatcgaaa gattcctgcc gagttttttc agatgaaccc tgttttccaa
     5281 caactgcccc ttcagaaaac gattatcaag ggcgatcagc tttgtgtctc aattgccgcg
     5341 gcctcgattc tcgctaaagt cgcacgtgat actatgatgg tcgagctcga caagacttat
     5401 ccggagtacg gctttgcgca ccacaagggg tatagctgct taatgcatat ggaagctttg
     5461 cgccgtctcg gaccatcgcc gattcatcgg cggagcttca aacccgtccg tgcgatgtgc
     5521 gcgctggacc tcgaaaccca tttagatttg ccgttcgtca atccgtgatc ctaaacaagg
     5581 ccagaatcat attaggtaag cagggtgaac ggcttgccga gctcttcttg aagaagaaag
     5641 gatacaagct cgtcgaacgc aactaccgtt gcgctgcggg agagctggac ctcattatgt
     5701 tggaccagcg cgtgatcgtt tttgtcgaag tgaagacgcg tacggggatc agatacggga
     5761 cgccgctgga agctgtcgaa gttcggaaac agcagaaaat gatttgcgcg gcgcagttct
     5821 ttgtgaccgc caaaaagctc aatcagcgtg aggccaggtt cgatgtcgtt ggcatttctt
     5881 ggcgcggcgc ccagccacac gtggaacaca ttgagaacgc attcgagcta gtctagggat
     5941 tttaaatgct ggatatcaga ctgctgcggg atgatctgga gcaagtgaaa acgcggatgg
     6001 cgactcgcgg tgtaaaaatc gattggggag aatttctctc gctcgaccga caacgccggg
     6061 atgcgctgag tagattggaa aagctcaaag agcagaaaaa ccgactttct ggcgagatcg
     6121 gcaatttgaa aaaaagcggc gcggatgcca cgggtttgat gcgggaagtt gagcaactta
     6181 gccagcaaat tcgtgagagc gaaaggccgc tggcggaagt ggaagaacga ttcgaacagt
     6241 tcatgttaac cgtaccgaac cttcctcaca tcaatgttaa ggttggcaag gattcagatg
     6301 ataatagaga aattcgccgc tggggtgcgc cgcccaagtt tgattttcat gcgcgaaatc
     6361 attgggaaat cggcgaggag ttggacattt tagacttcca gcgtgccgca aaactggccg
     6421 gcgcacgttt cgttatctat aagggcgcgg ctgcgcggct tgagcgtgca ttgatcaatt
     6481 tcatgctgga tctgcatacg ggcgagcacg gctacaaaga aatcattccg cccgcactgg
     6541 cgaaccgggc tgcgcttacg ggaaccggca atctgccgaa attcgaggag gatctctttc
     6601 agattgagag tccggaatat ttcttgatcc cgaccgccga agtgcctctc accaatgttc
     6661 accgcgagga ggtactccaa cgcgagtcgt tgccaataaa gtatacggcc gtgacgatgt
     6721 gttttcgccg tgaagcgggt tcttacggca aagatgtgcg cgggctgatt cggcaacatc
     6781 aattcaataa agtcgaaatg gtaaagctct gcgagccgga gttttcctat ggtgagttgg
     6841 agacaatggt gaaggacgct gaagaagttt tgaagcgact tgaattggcc tatcgggtcg
     6901 tagaactttg tactggtgat atgggatttg cctccgcaaa aacttacgac atcgaagttt
     6961 ggctgccggg tcaagacacg tatcgtgaga ttagttcgtg ctcgaactgc gaggattttc
     7021 aagcccgccg tgcacaaatc cgctatcgca aggagaacgg aaagccgaat tttgtccaca
     7081 cgttaaacgg ctccggactt gcggtaggcc gaactctcgt ggcgattttg gagaattatc
     7141 agcaaaagga cggcagcgtc ctaatcccga cggctcttcg gccgtacatg ggtggactgg
     7201 aaaagatcgt tcgagatggt cgtttagagt agctgcgtaa cccttcttcg gatgctttct
     7261 tgacctcaca tcgaagttca ctgaatgcat gcttgccgat gatgtcgcgg ctggaaaaag
     7321 cgcagagctt gatgctcaaa tcgacaatct cttgttgaag agagccctaa cgcagaacaa
     7381 gaagaaaatg gccaccaggt tcgtagaagc ttactt
//
LOCUS       DASUTG010000113         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_165700, whole genome shotgun sequence.
ACCESSION   DASUTG010000113 DASUTG010000000
VERSION     DASUTG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165700"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..487)
                     /locus_tag="VF089_02530"
     CDS             complement(131..487)
                     /locus_tag="VF089_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172862.1"
                     /translation="MPDIQLRPELALRTGGEIGRGVRKDTPKLKALLNEFITEHLKGT
                     QMAAVHLTTYQRRFKALHNATIAREWKKFEDTIAFFEKYGGKYGFDHPDAGRARLSGV
                     TAESVCAQPCRAIFRT"
     gene            complement(627..1019)
                     /locus_tag="VF089_02535"
     CDS             complement(627..1019)
                     /locus_tag="VF089_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172863.1"
                     /translation="MKLLLIVISSIALLGTGSASAGDFDGSKVLICAPVEAIDCAPGE
                     TCKKGAPADIGVPSFIRIDIAKMVVIGPKRTTPIVSIEKSEQQILLQGTELGFAWTLT
                     LDQDSGEMSGSLVNREEVFVLFGSCTPL"
     gene            complement(1058..1477)
                     /locus_tag="VF089_02540"
     CDS             complement(1058..1477)
                     /locus_tag="VF089_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6172864.1"
                     /translation="MTVSAQKPIVYPAKGQSAQQQTKDDGECYVWAKQTTGIDPAVVA
                     STPPPQQTGPAVGGGERVRGAARGALGGAAIGAIAGDAGQGAGIGAVVGTAAGGRRAR
                     QNRDAQNEQAVSQQQNLVDTYYRAYGACMQGRGYTVK"
     gene            complement(1805..2236)
                     /locus_tag="VF089_02545"
     CDS             complement(1805..2236)
                     /locus_tag="VF089_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX6172865.1"
                     /translation="MLYEPKKGSGEGIGIFGRFGASDGSPNFMHFFYSLGIGGKGVIP
                     TRERDRYGFGFYYIDVNNPELQGLFRNVKLLRDEYGFEAFYNVAITPWLMLTPDIQVV
                     RGAQRERFTIGQGPILGLPFIASRKSIGMATILGLRMQMVF"
BASE COUNT          502 a          693 c          570 g          565 t
ORIGIN      
        1 gagttataat gccgggccga gccgcatcgc aaagatccgt gccgcagcca agaagcaacg
       61 gctaaaccca aacaagtggt tcaacaaggt cgaacgtcgc ttcaaaacac gttggtcagg
      121 aacccgtgca ctacgtccgg aatatcgccc tgcacggctg cgcgcagact gattcagccg
      181 tgactcctga tagccttgcg cggccagcat cagggtgatc gaacccatat ttccctccat
      241 atttttcaaa aaatgcgatc gtatcctcga atttcttcca ctcacgagca attgtcgcat
      301 tgtgcagcgc tttgaaacgc cgttgatagg tcgtgagatg aaccgccgcc atctgcgtgc
      361 ctttaaggtg ctcggtgata aactcgttaa gcaaagcctt cagtttgggc gtgtctttgc
      421 ggaccccccg gccgatctcc ccgcccgtgc gcagcgccag ctcagggcga agctgaatat
      481 cgggcaagat cactgcccac agtttggctt tccaatcgtc aatgacgata acagaagcgc
      541 acccaatttt ttgatagata gggtctatgc tttttcacct taagtggagg tttggacccg
      601 caggagtttg agcatggcat tgggtatcag agcggcgtgc acgacccgaa cagaacgaac
      661 acttcttcgc ggtttaccaa cgatcccgac atctccccgc tatcctggtc aagtgtcagt
      721 gtccacgcga aacccaactc ggttccctgc aacaggatct gttgttcgct tttctcaatg
      781 gagacgattg gcgtagttcg cttcggcccg atgacaacca tcttggcgat atcgatgcgg
      841 atgaaagacg gcacgccgat atccgccggc gcgccttttt tgcacgtttc tcctggagca
      901 caatcgatgg cttcgaccgg agcgcaaatc aaaactttgg acccatcaaa atcgcctgct
      961 gaggctgatc ccgtcccaag caacgcgatc gacgaaataa ctataagtag caatttcatg
     1021 ttttcctcat ctatgccttg gtgatcgagt cttttcttca cttaacggtg taaccgcgtc
     1081 cctgcatgca cgctccataa gcgcgataat aggtatcaac gagattttgc tgctgactga
     1141 ctgcctgctc attttgcgcg tcgcgattct ggcgcgcccg gcgtccaccg gctgcggtgc
     1201 ccacaaccgc accgatgccg gcaccctgtc ctgcatcacc cgcaattgcg ccgattgccg
     1261 cgccgcctag cgcgccgcga gcggcgccgc gcacacgctc cccaccaccg actgcggggc
     1321 cagtctgttg tggcggcggt gtcgatgcca cgaccgccgg atcgatgccg gtcgtctgct
     1381 ttgcccagac ataacactca ccgtcatcct tggtctgctg ttgagcgctc tggcctttgg
     1441 ccggatagac gatcggcttt tgcgccgaca ccgtcatcgc gaagcatacc accgctacgt
     1501 ttatcgtgat ttgtactaat cttttcatgg tgacttcctt tctacgcgtg ctatagcttg
     1561 tttttacgct gttagctttc tgacccgcag atcctgattg cctgaaacga acgttcctta
     1621 gttgggtgat ccggatcgcc ggtaaaaacc ctgatcaaaa aagctcaaat ttttctctct
     1681 catcgatcct ccctctttcg agaaatgctt attgattcca gcgttttctt tcgacgctcg
     1741 ttcctctcgc gacaatactc aacaatcgct gctcggcgca ctggacgctt gcctctcgat
     1801 ccctctagaa caccatctgc atcctcaagc ccaaaatcgt cgccatgccg atactcttgc
     1861 ggctggcgat gaaaggcagt cccaggatcg gcccttgacc aatggtgaat ctttctcttt
     1921 gagcgcctct gaccacctgt atgtcgggcg tcagcatcag ccacggcgtg atcgcgacgt
     1981 tgtaaaaagc ttcgaaaccg tactcatcgc gcaacaactt gacgttgcga aacaaacctt
     2041 ggagctccgg attgttgacg tcgatgtagt aaaatccaaa gccgtagcga tcacgttctc
     2101 gagtcggaat aactcccttc ccgccgatcc ccagactgta gaaaaaatgc atgaagttgg
     2161 gactgccgtc cgaagcgccg aagcggccga agatgccgat gccttcgccc gagcctttct
     2221 tcggctcgta gagaatgttt caagaacctg ttttactttc gaacgcgccg ttctcgaaga
     2281 tgaagcgcgc ggtctgggtc gatcgatgtg aatttccgat tggaatacat
//
LOCUS       DASUTG010000114         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_17895, whole genome shotgun sequence.
ACCESSION   DASUTG010000114 DASUTG010000000
VERSION     DASUTG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17895"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VF089_02550"
     CDS             <1..1372
                     /locus_tag="VF089_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX6172866.1"
                     /translation="VGAGKAVISTPYWHAEEMLADQRGALVPFRDPTALADQVIDLLS
                     NDAKRHAMRKRAYMYGREAIWPQVARRYMETFQRARAERRHFAPSDFAVKPLDKRPGE
                     FPPLKLDHLQRMTDHTGMFQHAIFTVPNYVEGYTIDDNARALLVSALLEELGNPEAYE
                     LSSRYLAFVWYAFNSETRRFRNFMDYQRNWLEENGSDDSHGRTLWALGTLLGRTSTPA
                     LQSTAGRLFERALPLISDTTSPRAWAFALIGIHEYLRRFDGDRRASQVLEQLAGRLLN
                     LYEKNRAEDWRWYERSLTYCNAALPHALLMSGQRIPNYKMVEAGLESLHWLADLQRAD
                     TNGGHFVPIGSNGFYQRSGERARFDQQPVEAQAMVSACLEAHRVTGDKGWHNEARRAF
                     DWFLGRNDLNLPIYDPVTGGCRDGLHSDRANENQGAESSLAYLQAVLEMRLAETTLLS
                     VETGAR"
     gene            1369..2328
                     /locus_tag="VF089_02555"
     CDS             1369..2328
                     /locus_tag="VF089_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEX6172867.1"
                     /translation="MTNQHPELFRRQKTNPILSAADWPYPVNSVFNPGATVLADGTTL
                     LLCRVEDRRGHSHFTVARSINGVDNWQIEPQPTFLPDPEQFAEELWGIEDPRITYVPE
                     LSKYAVVYTAFTRDGPGVSLALTKDFHEFERYGVIMPPEDKDAALLPYRIDGQWALIH
                     RPVSAPRAHMWLSYSPDLRHWGNHKLMLEARRGAWWDANKIGLSPPPIETASGWLVIY
                     HGVRNTASGSIYRLGLALFDLHAPEHCLTRGDEWIFGPRETYEQRGDVDNVVFPCGYT
                     LAADGDTLRIYYGAADTSIGLATASLGAMVEWLKKHKSKIVEY"
BASE COUNT          497 a          670 c          694 g          530 t
ORIGIN      
        1 cgtgggtgcg ggcaaggcgg taatctccac cccatactgg catgccgagg aaatgttggc
       61 ggaccagcgt ggcgcgctgg taccttttcg cgatcccacg gcattggcgg atcaagtgat
      121 cgatttgtta agcaatgacg ccaagcgtca cgcgatgcgc aagcgcgctt atatgtacgg
      181 cagggaggcg atatggccgc aggtcgcaag gcgttacatg gaaacttttc agcgggctcg
      241 agccgaacgg cgccacttcg ctccatcgga ctttgccgtt aaaccgctgg acaaacgtcc
      301 aggcgaattt cctcctctca agcttgatca cttgcagcgc atgaccgacc acacgggtat
      361 gtttcaacac gcgattttca cggttcctaa ctatgtcgag ggttacacca ttgacgacaa
      421 tgcccgcgct cttttggtga gcgctctgct ggaggagctg ggaaacccgg aagcttatga
      481 actttcatcc cgttatctcg cgtttgtttg gtatgctttc aattccgaga cccggcgctt
      541 ccgcaatttt atggactacc agcgcaattg gctggaggag aacggctccg atgatagcca
      601 cggtcgcacc ttgtgggctt tgggtacttt gctgggtcga accagcactc cggcgcttca
      661 gagcacggcg ggccgcctgt tcgagcgggc tttgccgtta atcagcgaca ccaccagtcc
      721 ccgcgcatgg gcattcgcgt tgatcggcat acacgaatat ctgcgccggt tcgatggcga
      781 tcgccgggcc agccaagtgc tggaacagct tgccggccgg ctactgaatt tgtatgagaa
      841 aaatcgcgcc gaagactggc gctggtatga aaggtcgctg acctactgca acgcggcgtt
      901 gccgcacgcc ttactgatgt ccggccaacg gatacctaac tataagatgg tcgaggcagg
      961 cttggagtcg ctccactggc tggccgattt gcagcgcgcc gacaccaacg gcggtcactt
     1021 tgtgccgatc ggctctaatg gcttttacca acgcagcggc gagcgtgcgc ggttcgatca
     1081 gcagccggtg gaagcacaag ccatggtgtc tgcctgcctc gaagctcatc gagttacggg
     1141 agacaaaggc tggcacaatg aagcgcgacg cgccttcgac tggttcctcg gacgcaacga
     1201 tttgaacctg ccgatctatg atccggtaac cggcggatgc cgggacggct tgcattccga
     1261 ccgtgccaat gaaaatcaag gcgcagagtc ttctttggcc tatctccagg ccgtgctgga
     1321 aatgcgtttg gcggaaacaa cattattatc ggtggaaact ggagcgcgat gacgaatcaa
     1381 caccctgaac tttttcgccg gcagaagacc aatccgattt taagcgccgc cgattggccg
     1441 tatccagtta atagcgtctt caaccccggc gccacggtac tcgctgacgg cacgaccctg
     1501 ctgttgtgcc gggttgaaga tcgacgcggc cattctcatt ttactgtggc tcgttcgatc
     1561 aacggcgtcg ataactggca gatcgagccg cagcctactt tcctgcctga ccctgaacag
     1621 tttgccgagg agttatgggg catcgaggat ccgcgcatta cctatgtccc tgagttgagc
     1681 aagtatgccg tcgtttatac cgctttcaca cgcgacggcc cgggagtttc tctcgccctg
     1741 accaaagact ttcatgagtt cgaacgctat ggtgtcatca tgccgcccga ggacaaggat
     1801 gcggctttgc ttccgtaccg tatcgatggc cagtgggctt tgattcaccg cccggtcagt
     1861 gcccctcgag cgcatatgtg gttgtcttac tcgccggacc tgcggcactg gggcaatcac
     1921 aagttgatgt tggaagcacg tcgcggagct tggtgggatg ccaataaaat cgggctttcg
     1981 ccgccgccaa tcgaaaccgc tagcggctgg ctggtaattt atcacggcgt gcgcaatacc
     2041 gcgtcgggct ccatctaccg attggggctt gctctgtttg atctgcatgc gccggagcac
     2101 tgcctaacgc gcggcgacga atggattttt ggcccgcggg aaacctacga gcagcgtggc
     2161 gacgtcgaca acgtggtatt tccctgcggc tataccttgg cggcggacgg cgacactctt
     2221 cgaatctatt atggagccgc agatacgagc atcggcttgg ctaccgcgag tctgggggcg
     2281 atggtggagt ggcttaagaa acacaagtca aaaatcgtcg agtactagcc cggattattc
     2341 atggataagc gaataaagaa gccatcggca ccgtcgaact atgctaaaaa t
//
LOCUS       DASUTG010000115         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1648156, whole genome shotgun sequence.
ACCESSION   DASUTG010000115 DASUTG010000000
VERSION     DASUTG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648156"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..>2388)
                     /locus_tag="VF089_02560"
     CDS             complement(155..>2388)
                     /locus_tag="VF089_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6172868.1"
                     /translation="WRIFRRRPSLAVPFRSFPTRTAPAMKAAEQHPIKLAELNQQALL
                     DHFDASMVLIRQTGEILHFYGATDKYLAHPSGDATLNLFSMIDKHHAVALRLAAERAE
                     REDVPVTLQLREINRNDPADLVKLTVKPVKDPNSRKRLIGVIFQPAQPQPTPEPVPGQ
                     SGKPQDGDISAQLEAENVRLKQDLQSAIETFQVTHEEYTAVNEEVLAINEELQATNEE
                     LETSKEELQSVNEELVTVNNQLNEKVEDLSKANDDLANFLNSSDVATLFLDRGFCIRR
                     FTASATRLMNLLSLDVGRPIKDIASQLVNVNLTSVADAVLKNLASYEQEVAVANGSWY
                     MMRCVPYRTLNDVIDGVVFTFTDVTRLKQSEEAMRRALDYTDNIIKTVPVSLLVLDAN
                     LTVMRANQAFDRTFRVAREETEHRLIYELGSGQWNFPKLREVLEDVALHDSQIDRFEV
                     EHDFPGIGRKIMSLNARKLSSSGHDDGDSILLAIEDITGRRQAEAERLWLEGELRQAQ
                     KMESLGTLAAGIAHDFNNILNIVQGYAFVLRDSKIADELISESVSAILDSTKRGATIV
                     QQLLTLARKTEPKFELVNIDALVGELAQLYGKSSPQTIDIKLELSRQSPPLLADRNQI
                     FQALLNLCLNARDAMPSGGTLTLKTSVVNRNAVPSSEEDMAEQYVRIDVEDTGEGIDE
                     KVLSRIFEPFFTTKGAGRGTGLGLAVVYGILKHHKGFIQVMSKPGAGTSFQIYLPVTR
                     RTD"
BASE COUNT          476 a          675 c          678 g          559 t
ORIGIN      
        1 agatgtccac cgcttcctga ccgtcagccg ccagcagcac cgatgtttcc tcggtacaga
       61 gaatcctttc aagcaaccgg cgcatgagac gttcgtcttc gaccacaagg atagtgcgag
      121 agtggttatc gccaggtggc attggctcaa cccattaatc agtgcgtctc gtaaccggta
      181 aatagatttg aaaggacgtt cccgcgccgg gcttgctcat tacctggatg aaacctttgt
      241 ggtgcttgag aattccgtac acgaccgcca ggcccagacc ggttccacgg ccggcccctt
      301 tggtggtgaa gaaaggttca aaaatccgac tcaggacctt ttcatctatt ccctcgccgg
      361 tatcttccac gtcgatgcgc acatactgtt cggccatgtc ctcctcggag ctaggcacgg
      421 cattacggtt gaccaccgag gttttcaatg tcagggtgcc gccgctcggc atcgcgtcgc
      481 gcgcgttcag gcaaagattc agcagagctt gaaaaatctg attgcggtcg gccaacagcg
      541 gcgggctctg ccggcttagt tcgagcttga tgtcgattgt ctgcggagag gacttgccgt
      601 ataactgcgc cagctccccg accagagcat caatgtttac taactcgaac ttcggttccg
      661 tcttgcgcgc taaggttagt agctgctgca cgatggtcgc gccgcgtttg gtgctgtcga
      721 gaatcgcaga aacgctttcg ctgatcagtt catcggcgat cttggaatcg cgcaagacaa
      781 aagcgtatcc ctgcacgata tttaaaatgt tgttgaagtc gtgcgcgatc cccgccgcca
      841 gggtgccaag gctttccatc ttctgcgcct ggcgtagctc gccttcgagc cagagtcgct
      901 cggcttcggc ttgcctgcgc ccggtgatgt cctcgatcgc cagcagtatt gagtcgccat
      961 cgtcatgccc actgctggaa agcttccggg cgttcagaga catgatcttg cggccgatgc
     1021 cgggaaagtc gtgctctacc tcgaacctat cgatctgcga gtcgtgcagc gcgacatcct
     1081 ccagaacctc ccgcagtttc ggaaaattcc attggccgct gcccagctca tagatgagcc
     1141 ggtgttcggt ctcctcgcgc gcgacccgga aggtccggtc gaaagcctga ttcgcacgca
     1201 tcactgtgag gttggcatca agcacaagca gagaaacggg aaccgttttg ataatgttgt
     1261 cggtgtaatc aagcgcgcga cgcatcgctt cttccgactg cttgaggcgg gtcacatccg
     1321 tgaacgtgaa gaccacgccg tcgatgacgt cgttcagcgt gcgataggga acgcagcgca
     1381 tcatgtacca cgacccgttt gcgaccgcga cctcctgctc gtaactggcc agattcttta
     1441 gcacagcgtc ggccacgctg gtcagattca cattgacaag ctgacttgcg atatccttga
     1501 tcggccggcc gacatccagg ctcagtaagt tcatcagccg tgtcgccgat gcggtgaaac
     1561 gacggataca gaaaccccgg tccaaaaata acgtcgcgac atccgagctg tttagaaaat
     1621 tcgccagatc atcattggcc ttgctaaggt cttcgacttt ttcgttgagc tggttgttga
     1681 cggtgactag ttcctcattg accgattgta attcttcctt ggatgtttcc agctcctcat
     1741 tggtggcctg taactcctca ttgatcgcca gcacttcctc attgacggcg gtgtattctt
     1801 catgcgtcac ctgaaaggtt tcgatggcgc tttgcagatc ttgcttcaac cggacgttct
     1861 cggcctccag ctgggcggag atgtcgccat cctgaggttt gcctgactgc ccggggacgg
     1921 gctcaggcgt aggctgcggc tgcgccggct ggaagatgac cccgatcagg cgcttgcgcg
     1981 agttgggatc tttgaccggc ttgacggtga gctttaccaa gtcagcagga tcgttgcggt
     2041 tgatctcacg aagctgcaag gtaacgggta cgtcttcgcg ctcggcacgc tcggccgcca
     2101 ggcgcagggc aacagcgtgg tgtttgtcga tcatgctaaa aagattaaga gtggcatcgc
     2161 cggatgggtg cgccagatac ttatccgtcg cgccgtaaaa atggaggatt tcgccggttt
     2221 gcctaatcag gaccatgctt gcatcgaagt gatccaggag cgcctgctga ttaagctccg
     2281 cgagtttgat cgggtgctgc tctgccgctt tcatggcggg ggctgttcgg gtaggaaagc
     2341 tgcggaacgg aacagccaac gatggccggc gtcgaaagat gcgccagc
//
LOCUS       DASUTG010000116         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_166163, whole genome shotgun sequence.
ACCESSION   DASUTG010000116 DASUTG010000000
VERSION     DASUTG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166163"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF089_02565"
     CDS             complement(<1..239)
                     /locus_tag="VF089_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172869.1"
                     /translation="MQIELNYPDPLPLELDAGKTLLLIIDMENEDAHPLGKRFAGERV
                     TRIIPNIARLRHKVRQAGGSVVHTRSVRKPYALEFT"
     gene            512..1570
                     /locus_tag="VF089_02570"
     CDS             512..1570
                     /locus_tag="VF089_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172870.1"
                     /translation="MSTRNKLIGIASFFGLVFCVQSNDALSQSNFYAGKTVTLIATTS
                     AGGTGDLRVKAMVPFLKKHIPGNPTVVIQYIDGGGGRKGTNYLYSNARPDGLTIGAAS
                     GAIIGLAVMGETGISYDIDKFIYLGSPESENHYVIYTRKELGLDNLEKLASKPGLRIG
                     AQTIGHVSYVAGRLFAYFLNLKEPRFIAGYTAHELDVALTQGELDSRANNASSVIRRN
                     PDWVEKGIMNFHAIMEVPKGDKHPRLGHLPEIGSFAKNERENKLLTLWRAFRSVGSPY
                     ILPPGTPKERVATLEEAMRKTFEDPEFPPYFKKLVTDDASPMNAAELRKVVAEMPRDA
                     ETIDMLKKFAGSAPLPPR"
     gene            1730..2239
                     /locus_tag="VF089_02575"
     CDS             1730..2239
                     /locus_tag="VF089_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HEX6172871.1"
                     /translation="MIKKQWLRLGVALLPLYAAQVWAAPNPDTGPGCGLGKLAWGEYR
                     NQKDIAPQVMQSTTNGTFGSQTFGISFGTSGCTNNGVIMSEHKTSVFAALNFENLSQD
                     MAKGDGEYLSSLAELMNIPQENRSEFYALAQLQYQSMIQSGESTPAAMLKALTNAMSS
                     HPLLASSVK"
     gene            2261..4183
                     /locus_tag="VF089_02580"
     CDS             2261..4183
                     /locus_tag="VF089_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX6172872.1"
                     /translation="MPGLNPLSVILSIALLVLCFLPNTVRGQDSAQAYLQLLLDRAEA
                     MALHEERYWHLLLHYRSAFPGGIESEADEPGFFLAPNGKTDPEAELQATLTKFFSSDL
                     VGRSPQPARCAFIARYHWLKSALAIDESRLPQQRCERFQSWLTELNPDSITVIFPSAF
                     MNNPSSMFGHLLLRVDQKGQTERTSILAYTINYAADVTTENGILFAVLGLTGGFKGYF
                     STHPYYIKAKEYGDFENRDIWEYRLNLSEDQMQRLLMHAWEMGNATFDYFYFKENCAY
                     QILALLDVADPELQLAERFWFYTFPSDAIRIIADKPGLVNDVTFRAARSTRIRRGRET
                     LSAEEKSWLSKMVANPKLSQSEAFTRLPAERQAAVLEVVSDYLLYKGATDENSASFEA
                     RNKRILLARSKLKVRPVPTKVTPVSGAPDQGHKTMRAGLGVGWREGELFNEANFRLAF
                     HDLLDPEYGYTPGAQIQALGLTLRHYSRSNHTRLEKFTLLDILSLSPIDALFASPSWK
                     INAALDTIEHHNCRFCRIANLNAGIGIATESSWLNREIYFGMADLVTEVGPVFQGNHR
                     AGGGLTVGALTDITEHWKFAISGSYLNFPLGDKSDEWRLSVQQRYTLRRNFAVRFDFN
                     HRDRTQELVLNLQAFF"
     gene            4696..5712
                     /locus_tag="VF089_02585"
     CDS             4696..5712
                     /locus_tag="VF089_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172873.1"
                     /translation="MSVRKRVGTLLGAFLCISTMLLVTIAYAQESFRVSYGGYNETAA
                     PMWVGIDKGLFKKYGINASMIQVRSGALSIAALVSGEVEAVWPAQSTILSTVAGGVKL
                     GCVASATNKIPRQLVARKEIKSIEELRGRIIGVQSIGGGFWLQTMIILDHLGVDPDKY
                     GLKMRVIGDGPVIAQALMNGNIDAAALTYSLSEAPLKAGFRSLADAAELKAPYQGPSI
                     CALKELIGNRQDFFLRVTKGLVEATAYILDTNNKTEVMKSLQKHLRLQKVDEVEASYK
                     VLRLMATLDIAPNPAAYKSVQRIVATVNPKIAQVDIEQIMDSSFVRTLESSGFIAEQR
                     KRVR"
BASE COUNT         1561 a         1699 c         1683 g         1494 t
ORIGIN      
        1 gtaaattcca gcgcgtaggg tttgcgcaca gatcgagtgt gaactacgga tccgcctgct
       61 tgtcgcacct tgtgtcgcaa ccgcgcgata tttgggatga tgcgagtcac cctttcgcca
      121 gcgaagcgct tgcccaaagg atgagcgtct tcgttctcca tatcaatgat gaggagcaac
      181 gttttacctg cgtctaattc aaggggaagc ggatcgggat agttcagctc gatctgcatg
      241 atgtctcctg attagcggca ccccgacatc cagcaggaat cacgatcggt attgtcttat
      301 tactgctggt cggcggagaa ctaatggcac gtaaccggct tctaacacgc ccgcgttcta
      361 tcggtcaatt caaatgcata acgcgcggcc tggggtagtt catctgaggg atgacgataa
      421 tttgagaatc tgcttgtaaa agttaacaac tttttagata acaggttcta tcgaatgacg
      481 attgcttcca cactagccaa acggagatct tatgagtacc cggaacaaac taatcgggat
      541 cgcttccttc tttgggctag tcttttgcgt gcaatctaat gatgcccttt cgcagagcaa
      601 cttctatgcg ggtaagaccg tcaccttgat cgccaccacc tcggcaggcg gcaccgggga
      661 tctgcgggtc aaagccatgg tgccgttctt gaaaaaacat atcccgggaa atccaactgt
      721 cgttatccag tacatcgacg gcggcggcgg ccgtaaggga acgaattatc tctacagcaa
      781 tgctcgtccc gacgggctaa caattggcgc cgcgagtgga gcgatcatcg gcctggctgt
      841 catgggcgag accggcatct cgtatgacat cgacaagttt atttatctcg gctcacccga
      901 aagcgaaaac cattacgtta tttatacgcg aaaagaactc gggctcgaca atctggagaa
      961 actcgcatca aagcctggcc tgaggattgg ggcgcaaacc attggccacg tatcttatgt
     1021 agcaggaaga ctattcgcgt actttctaaa cttaaaagag cccagattta tcgccgggta
     1081 cactgcccat gaactggacg tcgccttgac tcaaggcgag ctcgacagca gagccaacaa
     1141 cgcaagttcg gtcatcaggc gaaatcccga ttgggtggag aaagggataa tgaattttca
     1201 tgcgatcatg gaggttccca aaggcgataa gcatccgcga ttaggccacc ttccggagat
     1261 cggcagcttt gccaaaaacg aacgggaaaa caaacttctt acgttgtggc gcgcttttcg
     1321 ctctgtggga tcgccttaca tactaccgcc gggtactccc aaagagagag tggcaactct
     1381 agaggaagca atgcgcaaga ccttcgaaga tcctgagttt cctccgtatt ttaaaaaact
     1441 ggtcaccgat gatgcatccc caatgaatgc cgcggaatta agaaaggtcg tcgcggagat
     1501 gccccgtgac gccgagacga tagacatgct caagaaattt gccggatcgg ctcctctacc
     1561 gccgcgctag ccgatatatg aaactgaggc gccgaatccg gctggtgtct cacctgatcg
     1621 tcagcaattc acagcgagat tatttttagt attgatttaa atgttcttgc tcgaagatgt
     1681 tcttcaagtt agattcagag tcatagaaat aacagaagga gggggatata tgatcaaaaa
     1741 acaatggctc aggttaggcg tcgctctgct gcctttatat gcggcgcagg tttgggcagc
     1801 accaaatccc gataccggac cgggttgcgg tctcggaaaa cttgcgtggg gggaatatcg
     1861 gaatcagaaa gacatcgcac cccaagtgat gcagtcgacc actaatggca cattcggcag
     1921 ccagaccttc ggcatcagct tcggcacttc cggatgcacc aataatggtg tcattatgtc
     1981 agagcataaa accagtgtgt ttgcggcgct aaattttgag aacctatctc aagatatggc
     2041 caagggcgac ggtgaatacc tgagctcgct cgctgaactg atgaacattc cccaggagaa
     2101 tcgttcggag ttctatgctc tcgcgcagct gcagtaccag agcatgattc agtcgggcga
     2161 atctactcca gcggcgatgc tcaaggcgtt aaccaacgca atgtcatcgc acccgcttct
     2221 cgccagcagt gtaaaataac ctatctatga gccccggcca ttgccggggc tcaatccctt
     2281 atcggtgatc ctcagcatcg cgcttctcgt cctgtgtttt ctgcccaaca cagtccgcgg
     2341 ccaagacagc gctcaagcct accttcaact gctgcttgat cgtgcagagg cgatggcgct
     2401 gcacgaggag cgctattggc atcttcttct gcactatcgc tcggcctttc cgggcggcat
     2461 tgaaagcgaa gccgacgagc ccggattctt tcttgcgccc aacgggaaaa ccgatccgga
     2521 agccgagctt caggctaccc tgacaaagtt cttttcgagc gatctggttg gccgttcgcc
     2581 ccagccggcg cgctgcgcct tcatcgcgcg ttatcattgg ctgaagagcg ccctcgccat
     2641 agacgagagc cggttgccgc aacagcgatg cgagcgtttt caatcatggc tcaccgagct
     2701 caatcccgac tcgattaccg tcatctttcc gtcagcattc atgaacaatc catcgtcgat
     2761 gttcggccac ctgctcttaa gggttgacca gaagggtcaa accgagagaa ccagcattct
     2821 ggcgtatacg atcaattatg ccgccgatgt caccacggaa aacggcatcc tcttcgctgt
     2881 gctaggactt actggaggat tcaaaggata cttttcgaca catccctact acatcaaggc
     2941 caaggaatac ggtgatttcg aaaaccgaga catctgggag taccggctca atctctcgga
     3001 agatcagatg caacgcttgc tgatgcacgc ctgggagatg ggcaacgcca ctttcgatta
     3061 cttttatttc aaagaaaatt gcgcctatca gatactggcc ctgctggatg tcgctgatcc
     3121 ggagctgcaa ctggcagaac ggttttggtt ctataccttc ccttccgacg cgattcgcat
     3181 aatcgccgac aagcccggcc tggttaatga tgtgaccttt cgcgccgccc ggagcaccag
     3241 gatccgccgc ggccgggaga ctctttcagc cgaagaaaag agctggctct ctaagatggt
     3301 cgccaatccg aagctctctc aatctgaggc gtttacccgc ctacccgccg agcgccaggc
     3361 tgcagtactc gaggtcgtat cggattatct tttgtacaaa ggcgcgacgg acgagaacag
     3421 cgcatcattc gaagcgcgca ataagaggat tttgcttgct cgcagcaagc tcaaggttag
     3481 gccggttccg accaaggtca cgccggttag cggcgcgccc gatcaaggac acaagactat
     3541 gagagccggg ctgggggttg gctggcgtga aggagaactt ttcaacgagg cgaattttcg
     3601 cctggctttt cacgatctgc tcgatccgga atacggctat acgcccggag cgcaaatcca
     3661 ggcattggga ctgactttgc gtcactacag ccgaagcaat cataccaggc tcgaaaaatt
     3721 taccctgctc gacattttat ctctgtcgcc gatcgatgct ctctttgcga gtccttcatg
     3781 gaagatcaat gcggcgctcg acacgatcga gcatcacaac tgtcgctttt gccgcatcgc
     3841 caacctaaat gctgggatcg gcatagccac cgaaagctcc tggttaaatc gcgagatcta
     3901 ttttggcatg gcggacttgg ttactgaagt cggacctgtg tttcaaggca atcaccgggc
     3961 cggcggcggc ctcacggttg gcgcgctgac cgacatcacc gaacactgga agtttgcaat
     4021 ttccggcagc tatctgaatt tcccgttagg cgacaaatcc gacgagtggc ggttatcggt
     4081 gcagcaacgc tataccttga gaagaaactt tgcggttcga tttgatttca atcaccgcga
     4141 ccgcacccag gagcttgtgt taaacctgca ggcattcttc taattccact gctttaaccg
     4201 caggctccgg attacgaagc ggtatatact tggcatcaag cccgctccgc ctcgaggtca
     4261 gtaacctcgt caattcatgc gtgcggtgag cgcgcgacat ctccagccat ctctctatga
     4321 gatgattcga accctatatt cggcgagaat actgagtatc ctcgtcggat agtacgccaa
     4381 gtattgtgaa caacggttga gttgattgcg ttccgctaag cggttggatt gaagctccta
     4441 tattcgattc ctagttgtcc tgctcattcg agcgggcgga aactgcccgc gcacaaaaga
     4501 cctgctgatt ataaactctg ggcgagcgtt ttcttgtaag cgaaaatcat gtaagaggac
     4561 agctgattga tgatcaactc attgtaacgc aaccgtggtg tgctacgaca tcacgccgtt
     4621 tgaactattc gaacggttaa ggcgagcttt gaaccgtcaa tgcatttcgg gttccggact
     4681 aacaggagga ctgcgatgag tgttaggaaa cgagttggta ctctgcttgg cgcgttcctg
     4741 tgcatctcta caatgcttct tgtcacgatt gcctatgctc aggaaagctt tcgcgtgtcc
     4801 tatggtggct ataatgaaac agcagcgccg atgtgggtcg gcatcgataa gggtttgttc
     4861 aagaaatacg gaatcaatgc ttcgatgatt caggtgcgca gcggcgcgct cagcatcgcc
     4921 gcgctggtgt caggcgaagt tgaagcggta tggcccgctc aatcgacgat tttgagcacg
     4981 gtggccgggg gggttaagtt gggctgcgtt gccagcgcta ccaacaaaat tccgcgccaa
     5041 ctggtcgccc gtaaagaaat caagagcatc gaggaattac gcggtcgcat catcggggtg
     5101 cagagtatcg gcggcgggtt ttggctgcag accatgatta tcctggatca cctcggagtc
     5161 gatcctgaca aatacggatt gaagatgcgc gtcatcggcg atggaccggt gatcgcgcaa
     5221 gcgctaatga atggcaacat cgatgccgcc gccctgacct acagtctaag cgaagcgccc
     5281 ctcaaggcgg ggtttagatc gctggccgat gccgcggagt tgaaagcgcc gtaccaggga
     5341 ccgagcattt gcgcgctcaa agagctcatt ggcaaccgac aagatttctt tctccgcgtg
     5401 accaaaggtt tagtcgaagc aaccgcttac attctagaca ccaataacaa gaccgaggtg
     5461 atgaaaagtc tgcaaaagca tcttcggctg cagaaagtgg acgaagtcga agcctcatac
     5521 aaagtgttac gattgatggc gactctcgac atcgcgccaa atccggcggc ctacaagtct
     5581 gtccagagaa ttgtcgcaac tgtaaacccg aagatcgcac aggtcgatat cgaacagata
     5641 atggatagca gtttcgtccg tacactggag agtagcggat ttatcgccga gcaacgtaaa
     5701 agggtgcgtt gattactatc gagagctaga gcgagctaat gtccgcatcg cgattcgcta
     5761 tcaacgcgag attgattctc taaggttaaa ttgtcttgtg gatattgcct ccagcgaggc
     5821 gcctaagctc cttgaagata gcatcacatt ctattcatga tcagatcccc gtggagcccg
     5881 tcattcaatg ccgttgcacg aatcacacga gctgactgat tcacgatgac ggaattgcga
     5941 acggatcttc cgtaaatacc cgccgttaag cttatcaccg cagctttctc tgcagtctca
     6001 aagccggctg acattgctgc cgctcgaaca agtaaccgcg aggtcatcgt gcacagtttt
     6061 cgccgtattg aatgcggtat gcagcactct catacaatct tgtccgcgat tgaccatcga
     6121 tattaccgca ctgccgttca ttgaggcaca cgacctgatc ccttcatccg gatcggtgag
     6181 ctgtcgcaac aatttcggtg caactcggta aaaaattcat gaccagttcc atccaactgc
     6241 cggaccagtg cggcaatatc cgctacttac aatcgcggat ccgtggcatg tacgttgctc
     6301 caggtgcgta catccaagtg cagtcaacca gaactagatt ggaggcaaag tgaaacagaa
     6361 cttaaatcag cttaggtcgt ctcgatgggt ggttgcggtg ggctttgctg cactcaccgc
     6421 tgtcggctgt gcgggag
//
LOCUS       DASUTG010000117         2640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_462288, whole genome shotgun sequence.
ACCESSION   DASUTG010000117 DASUTG010000000
VERSION     DASUTG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462288"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1034
                     /locus_tag="VF089_02590"
     CDS             321..1034
                     /locus_tag="VF089_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6172874.1"
                     /translation="MKLSILIMSLLLPASSLHAQENTRIRPPSVTAQGEAVITAEPDQ
                     AQIDIGVLTQARNAPDAAKENAEKLSRVLAQIKKLLGKGDEVKTTGYSLNPNYRYPQG
                     GKPEITGYTAANVLHIKTGNLTGVGKLIDASMQAGANQINRLVFTLKDEHSAQVQALR
                     MAAQEAQSKAEEMAGSLGLKVVRVLSLTENERGVRPIIAQTRGVQAEALSSAAPTPVE
                     AGTVEVRSSVTLTAEIAPR"
     gene            complement(1120..2565)
                     /locus_tag="VF089_02595"
     CDS             complement(1120..2565)
                     /locus_tag="VF089_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6172875.1"
                     /translation="MADGHEKKFAMENIESNSESIALLCDNIFYFAELGMQEFETSGL
                     MMQILEKAGFTIERNPSGMPTGFVASYGSGKPVIALHTEFDATPGCSQAPGVAEPTPI
                     VDGAPGHTEGHNVNGAVMIGAALAIKKTIDEFGVKGTLKVFGAPAEEQLVSRPYFVRD
                     GYFKDVDVALHDHIGKELGTVYGLRQYALISAEFTFKGQSAHAATAPWNARDALDAAV
                     LMDIGWDKLREHLEPTQRSHRVITNGGDQPNVIPNRSTIWWFFREATAEKTRELFEKA
                     KRVAQGAAMMTETSYDVNVLSAVWPTRANRTLAEVIQRNIEEIGMPQWTEGEQKLVHE
                     VQTKVKAKPTGMPTKVAPLKECVQSVSANDSGEVSWVVPTGLITFPSNIPGVSYHHWS
                     AGVSLATSIAHKGAVAGAKAMAASAIDLLLDPQVVAKAKETFKQEIGDVEYRPLLPPD
                     QKPPLELNRDLMERYRPKMREHYLKDRPEFR"
BASE COUNT          608 a          795 c          673 g          564 t
ORIGIN      
        1 aacccgacgc cgacaatata ccagccgtag aagaacttct gcttggatgc gggacgtgcc
       61 tgttcgattg tcgactccac gtctagccat taacaggaat caaactaaac attcaattaa
      121 caagtgattt ccgacctagg ccggggttag actttgcttc ataggggatt actcaacggg
      181 aacgccacgt cagaagatca ggaaagcgca atggcataga ctatgcgcct cagctagcat
      241 tgtggcagca gagccgatgt ctgctggcgg tgctctcgtc tatgcgatag cctcgtctaa
      301 taccaaagga cttatctcac atgaaactat cgatactcat aatgagtctc cttctgccgg
      361 catcgagcct ccacgcgcaa gagaatactc gcatcagacc accctcagtc actgcgcaag
      421 gcgaggcggt catcaccgcg gagccagatc aggcgcaaat cgacatcggg gtcctcacgc
      481 aagcccgcaa tgccccggat gcagccaaag aaaacgccga gaagcttagc cgtgtgctgg
      541 cccagatcaa gaaactcctc ggcaaaggcg acgaggtcaa aaccaccggc tactcgttga
      601 atcccaacta ccgctatcca caaggcggca agccggaaat aaccggctac acagccgcca
      661 atgttttgca catcaaaacg ggaaacttga caggagtcgg caagctaatc gacgccagca
      721 tgcaagccgg ggctaatcag atcaaccgcc ttgttttcac attgaaagat gagcattcag
      781 cgcaagttca ggcgctccgc atggcggcgc aagaggcgca gtcgaaggcc gaagaaatgg
      841 ccggctcctt agggttgaaa gtcgtcagag tcctatctct aacggaaaat gaacgaggag
      901 ttcgcccgat catagcgcag acacgcggcg tgcaagcaga agcactgtca tctgccgcac
      961 cgacgccggt cgaggccggc actgttgagg tgcgctcctc agtgacactg acggccgaga
     1021 tcgctccccg ctaagcgctg acattccggg acagcagcac agcactcttt tcctgaaacc
     1081 gtttgaactt ctgaacgact tgaaccgttg aacaattctc tatcgaaact ccggcctgtc
     1141 ttttaaataa tgctcacgca tcttcggccg gtacctttcc atcaaatctc gattcagttc
     1201 cagcggcggt ttctggtcag gcggcagtaa aggccgatac tcaacgtcgc cgatctcttg
     1261 cttgaaggtt tccttggcct tcgcaacgac ctgcggatcc aacaacaaat cgattgccga
     1321 cgccgccatc gcttttgctc cggccaccgc acctttgtgc gcaatcgacg tggccagaga
     1381 cacgccggcg gaccagtgat ggtacgatac gccgggaata ttcgatggaa atgtgatgag
     1441 cccggtcggc accacccacg agacctcgcc cgaatcgttg gccgaaaccg actgcacaca
     1501 ttctttgagc ggcgcaacct tggtgggcat gcccgttggc ttagccttca ctttggtctg
     1561 aacctcgtga actaacttct gctcgccttc tgtccactgc ggcatgccaa tctcttcgat
     1621 gtttcgttga atcacctccg ccagcgttcg attggcccgg gtcggccaga ccgcgctgag
     1681 aacattcaca tcgtacgagg tctcggtcat catcgccgcg ccttgggcaa cacgcttagc
     1741 tttttcaaac agctcccgcg ttttctccgc cgtggcttca cggaaaaacc accagatcgt
     1801 cgaccgattg ggaatgacat tgggctgatc gccgccgttg gtgatcaccc gatggctgcg
     1861 ctgggtcggc tccaaatgct ctcgcagctt atcccagccg atatccatca gcaccgccgc
     1921 atccaaagca tcgcgcgcgt tccacggcgc ggtggcggca tgggcgctct gtcctttgaa
     1981 agtgaactcg gcggagatga gcgcatactg tcgcaagccg taaacggtgc caagctcttt
     2041 gccgatatga tcatgcagcg ccacatcaac atctttgaaa tagccatcgc ggacgaaata
     2101 cggtcggctg actagttgct cttccgcggg ggccccgaaa actttcagtg tgcccttgac
     2161 gccgaactca tcgatggtct ttttgatcgc caacgccgcg ccgatcatca ccgcgccatt
     2221 gacgttgtgt ccctcggtgt gacccggcgc gccatcgaca atcggcgtgg gctccgccac
     2281 accgggcgcc tgtgagcacc ccggagtcgc atcgaattcc gtgtgcagcg caatcaccgg
     2341 tttgccagac ccgtaactgg ctacgaaccc cgtcggcatt ccggatggat tgcgctcgat
     2401 ggtgaatccg gctttctcga ggatctgcat catcaatccg gaggtctcaa actcctgcat
     2461 gccgagctcg gcaaagtaaa agatgttatc acagagcagc gcgatcgatt cactgttgct
     2521 ctcgatgttt tccatggcaa atttcttttc atgaccatca gccataattg gtctcctgtc
     2581 caaaagatgc tttttttcga tatcacggag cgaggaacaa cttaaagtca actcttactc
//
LOCUS       DASUTG010000118         6295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_462457, whole genome shotgun sequence.
ACCESSION   DASUTG010000118 DASUTG010000000
VERSION     DASUTG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462457"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VF089_02600"
     CDS             <1..657
                     /locus_tag="VF089_02600"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6172876.1"
                     /translation="FGGPAEYHFEKLLELGKIWETPRLTFKSYPCGSISHPYMDCAFK
                     LRHKYAPAPERIVEVVCRTAQGPVHRLWEPLADKQQPSSSYGAKFSLPFCIAVMLIRG
                     RAGLEEFTDAAIRDASVLALAKKVRYELDPSIDYPRHFSGHVQIRLEDGSVLEENQSH
                     PRGGFEDPLPPEEIQSKFRANAQLRLPPARADQIVAAVNRLEELAEIRSLTDLLKPGK
                     "
     gene            713..1732
                     /locus_tag="VF089_02605"
     CDS             713..1732
                     /locus_tag="VF089_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6172877.1"
                     /translation="MKLKSWLFVLALCFVTASLARAQDAQLVQAARREGKVVWYTSLA
                     LPSSTAISHYFENKYKGIQVEVHRNGSQRVLQRFMQEASAGLKNGDIVHTSDAGHFEL
                     LKDKGMLLKFTPEGVAAFPDGFKDKAGFYYGMRATLSVMAHNPKIVLEKDAPQGWKDL
                     LNPKWSGKMVTAHPGYSGIIMTHVLALVNVYGWDYFRDLAKNKLHIVQSANDPAGVVA
                     SAERPVGVNGAEYFYYKTLKQGNPIKIIYPKEGVPLVVSPVAIAKDAPHPNAAKLFTE
                     FIFSKESQQLLADKEGLYTGHPEVTYPADKPKLKDLKLLTADADELEKRNAEIKKRFV
                     EFFGA"
     gene            1751..3478
                     /locus_tag="VF089_02610"
     CDS             1751..3478
                     /locus_tag="VF089_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6172878.1"
                     /translation="MPASQTGTVGRIGQTGWAALRLRDPTVPVWIGAALLLVFLMLLP
                     LGAIFRASLWDDTGITFGRYLEVFSNEQFLKAIWNTVIISTWVGVVAVIIGALLAWLV
                     TRTDLPWKKSIRALVMASFVTPPFLGAFAWTLLAGPNAGALNKLYRAVTGSEEAIFNI
                     FTMPGLIFVMTLYSFPFVFSMIANVCELISSDLEDAAEILGANKWRTAWTVTLPLALP
                     ALIGGFILAFLHSLSLFGAPAILGLPAGLHTITTQIWTLFQYPPRLDMAAAFSVPLLL
                     ATMVLIVAQKKILGRKGYSTVGGKGGQKRLIRLGWGRVPVLLFVLGILSLSVFLPYWI
                     LLKAALSKAWALPLTWDNFTLHNLSFTFFEYGDTQLAIYNTFKLGLITATVGTLLATL
                     IAYITNRNLFRGARYLSFFALAPLVIPGIVLAVGLFIAYTRPPLVLYGTIWILFVAFL
                     TKEMPIGFSQAESTFKSIHPELEDASRIIGANRLTTLKDITAPLARSGLIAAWSFIFI
                     GVIRELSAAILLFAPQSKVVSVVIFDLKEEGQIGVIAVLGILLLLVSFAVIITVQRLA
                     GRDVVATRE"
     gene            3482..4576
                     /locus_tag="VF089_02615"
     CDS             3482..4576
                     /locus_tag="VF089_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6172879.1"
                     /translation="MAIVSIRGLTKKFTDAAAVDDLHLEIADGEFVSLLGPSGCGKTT
                     TLRLIAGFLQPDAGEICVGGGVISSPSHLVPPERRNMSMIFQSYAVWPHMTVAQNVAY
                     GLKFKKLTKQQIDQKVTKLLGVVHLSELKDRYSSELSGGQQQRVALARALVVEPQILL
                     MDEPLSNLDANLREEMRFEIRRLHEEFAITTVYVTHDQSEAMATSDRIAVLDRGQIVQ
                     VGRPAEIFDRPKTRFVAEFVGKANILSGRLDGGSRMQIAERLEIGVMPNPDVASTTGE
                     AAICLRPHNIVLTAVQSEAETLARQDYNVFSGVVQRRIYFGESADYTIDLAPHPFTLR
                     VVGSPSKLYDKGQRIFALARPEHCVVVSNH"
     gene            4797..6041
                     /locus_tag="VF089_02620"
     CDS             4797..6041
                     /locus_tag="VF089_02620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6172880.1"
                     /translation="MPDYVKEHYDFPLTQKSENKPLKGVRVIDAGNMVAAPFATVLLA
                     DFGADVIKIEHPKYGDGQRKLEPIMNGIPLWWKVVARNKRCITLDLGKPEGAEIFKQL
                     IKGKDAIVENYRPGTFEKWGIGPDVIRRIDPRIILLRISGFGQTGPYKNRAGFGRVAE
                     AMSGLTNLIGEPDGPPMSPGYPLGDLIAGMFGSLSIMMALYHRDLRGGEGQVIDLALF
                     EAVFRFLDFDPIQYDQMKTVHMRTGNRVAYVAPSSMFKTKDGKYLTLAASTQNVWERL
                     ADAIGRKDLITDPKFIDNPARVENSVECNGIVGAWIGQHTRDEVIEQFDKFGVAYSAV
                     FDMEDAFRDLQYRARAAMVRVPDPDLGEAVVQNVVPKFSATPGSVDFLGAKMGAHNEE
                     IYGSELGLSKERLRELKEAGVI"
BASE COUNT         1347 a         1720 c         1782 g         1446 t
ORIGIN      
        1 tttggcgggc ccgccgaata ccacttcgag aagttactcg aattgggcaa gatctgggaa
       61 acaccccggt tgacgttcaa gtcttatccc tgcggctcca tatctcaccc ctacatggac
      121 tgcgcgttca agctcaggca caagtatgcg cccgcgccgg aaagaatcgt tgaagttgtc
      181 tgccgtacgg cacaggggcc ggtgcatcgt ttatgggagc cgcttgccga caaacagcag
      241 ccgtcaagct cctatggcgc aaaatttagc ttgccctttt gtattgcggt catgctcatt
      301 cgcggccgcg ccggactcga agagtttacc gatgctgcca tccgcgatgc ttcagtgctg
      361 gcgctggcaa agaaagttcg ctatgagctc gatccaagta tcgattaccc gcgccacttt
      421 tccgggcatg tgcagattcg tctcgaagac ggcagcgtct tagaggagaa tcagtcccat
      481 ccgcgcggcg ggtttgaaga tcccctgccg ccggaggaga tccaaagcaa gttccgggcg
      541 aacgcgcagc tgcgtttgcc gccggcacga gcggatcaga tcgttgccgc agtcaatcgc
      601 ctggaagaac tggctgagat tagatcactg acggatctgt tgaaacctgg aaaataggag
      661 tagagtcggc aatgaattgc ggttgatcga gatcgataag gaggagcgaa tcatgaaact
      721 gaaatcctgg ttattcgtgc ttgccttgtg tttcgttaca gcttccctgg ctcgcgctca
      781 agatgctcag ctcgtgcagg cggcgcgaag ggaaggcaag gtcgtctggt acacatccct
      841 agcattgcca tcctccactg cgatctcgca ctattttgaa aacaaataca aaggcatcca
      901 ggtggaagtg catcgcaacg gttcgcaacg ggtgctgcaa cgatttatgc aggaagcgtc
      961 ggcgggattg aagaatggcg atatcgtgca tacttcggac gccggtcact tcgagctgtt
     1021 gaaggacaag ggaatgctct tgaagttcac acccgagggt gttgccgctt ttccggacgg
     1081 ctttaaggac aaggctggct tctactatgg catgagggcg acgctttcgg tcatggctca
     1141 taacccgaaa atcgtcttgg aaaaggacgc cccgcaaggt tggaaggatc tattaaatcc
     1201 gaaatggagc gggaaaatgg tcaccgccca ccccggctat agcggcatca tcatgacgca
     1261 tgtgctggcg ctggtgaacg tatacggttg ggactacttc cgcgatctgg caaaaaataa
     1321 attgcatatc gttcaatcgg ccaacgatcc agcgggcgtc gtcgcatcgg cagagcggcc
     1381 agtaggcgtc aacggagcgg aatattttta ttacaagact ttgaagcagg gcaatccgat
     1441 caaaatcatc tatcctaaag agggggttcc gctggtggta tcgccggtgg ccattgccaa
     1501 agacgcgcct catcccaatg ccgcgaaact cttcaccgag tttatttttt ctaaggagtc
     1561 gcagcagctg ttggcggaca aagaaggtct ctataccggc catcccgagg tcacctatcc
     1621 ggcggataag cccaagctca aggatctaaa gctgctgaca gccgacgctg acgaactgga
     1681 aaaacgcaat gccgagataa aaaaacgctt cgttgaattt ttcggcgcat agccgcgcgg
     1741 agaaacattc atgcccgctt ctcaaaccgg gaccgttggt cggatcggcc aaacgggctg
     1801 ggccgcgcta cgattgagag atccaaccgt gcccgtatgg atcggcgcgg cgctccttct
     1861 tgtttttctc atgctactcc cgctgggcgc gatttttcgc gccagtctgt gggacgatac
     1921 cggcatcacg ttcggcagat atcttgaagt cttttcgaac gaacagtttc tcaaagcgat
     1981 ctggaacaca gtgattattt ccacttgggt gggggtggtc gcggtgatca tcggcgctct
     2041 gctcgcctgg ctagtgacac gcaccgacct gccgtggaaa aaatcgatcc gcgcgctcgt
     2101 gatggcctcg ttcgtgacgc caccgttcct cggagcgttt gcctggacgc tgctcgccgg
     2161 acccaacgcc ggtgcactga acaagctcta ccgcgccgtc accggctcgg aggaggcgat
     2221 ctttaacata tttacgatgc ccgggctgat tttcgtcatg accctctaca gttttccctt
     2281 cgttttttcc atgatcgcca atgtgtgcga gctgatctcg tctgatctcg aagatgccgc
     2341 tgagattctc ggcgcgaaca aatggcgcac agcatggacc gttaccttgc cgctggcgct
     2401 gccggcgctg atcggcggct tcatcctggc ttttcttcat tcgctgtcgc tctttggcgc
     2461 gccggccata ctcggcctgc ccgcgggctt gcacacaatc accacacaga tctggacgct
     2521 ttttcaatac ccgccgcgcc tagacatggc cgcggctttt tccgtgccgc tgcttttggc
     2581 gacgatggtg ctgattgtcg cgcagaaaaa aattctcggg cgcaaaggct acagcaccgt
     2641 cggcggcaaa ggtggacaga aacggctgat ccgcctgggc tggggccggg ttcccgtgct
     2701 gcttttcgtg ctgggaattc tttcgctttc ggttttcttg ccctattgga tcctgctcaa
     2761 ggcggcgctc tccaaagcct gggcgctgcc tttaacgtgg gataatttta cgctgcacaa
     2821 tctttcgttt acttttttcg agtacggcga tacccagctg gcgatctaca atacttttaa
     2881 gctgggactg atcacggcca ccgtcggcac cctgttggcc acgcttatcg cctacattac
     2941 caaccgcaat ctgtttcgtg gggcgcgcta tttgagtttt ttcgcgctgg cgccgctggt
     3001 catcccaggc atcgttctag ctgtgggtct gttcatcgcc tacacgcggc cgccgctggt
     3061 cctctacggc acgatatgga ttcttttcgt ggctttttta accaaagaaa tgcccatcgg
     3121 gttttcccag gcggagtcta ccttcaagag cattcacccc gagctcgaag atgccagccg
     3181 gattatcggc gcaaaccggc tgaccacgct taaggatatt accgcgccgc tggcgcgctc
     3241 gggattgatc gctgcctggt cgtttatctt catcggcgtc atccgcgagc tgagcgcagc
     3301 gattctctta ttcgcgccgc agtcgaaagt tgtttccgtg gttatcttcg acctcaagga
     3361 agagggccag ataggagtca ttgccgtttt aggaattctc ttgctgttag tttccttcgc
     3421 ggtgatcatc accgtccagc ggctggcggg acgggacgtg gtggcgactc gggaatagtt
     3481 catggcgatc gtctcaatac gcggactcac taaaaaattt accgacgcag ccgccgtcga
     3541 cgatctccat ttggaaatcg ccgacggcga attcgtctca ctgctcggtc cctccggttg
     3601 cggcaaaacc acgacgcttc ggttaatcgc cggatttctg cagcccgatg ctggggaaat
     3661 ttgtgttggc ggtggagtca tctcgtcgcc atcccatcta gtgccgccgg agcggcgcaa
     3721 catgtcaatg atctttcaaa gctacgccgt gtggccgcac atgacggtgg cgcaaaacgt
     3781 ggcctacgga ctcaagttta aaaaactcac caagcagcaa atcgaccaga aagtcaccaa
     3841 attgctcggc gtcgtgcact tgtcggagtt gaaagaccgc tactcgtcgg agctctccgg
     3901 cgggcagcaa cagcgtgtgg cgctggcgcg ggcgctggtg gttgagccgc aaattttgct
     3961 catggacgag ccgttgagca atctcgatgc gaatctgcgc gaagagatgc gcttcgagat
     4021 ccggcgtctg cacgaagaat tcgctatcac cacggtttac gtgactcatg atcaaagcga
     4081 ggcgatggcg acgtcggacc gcatcgccgt gctcgatcgg ggtcaaatag tccaagtcgg
     4141 gcgaccggct gaaatcttcg atcgtcccaa aacccgcttt gtggcggaat tcgtcggcaa
     4201 agctaatatt ctctccggtc gactagatgg tggcagtcga atgcagatag ccgagcggct
     4261 ggaaattggc gtaatgccca acccggatgt cgcctcgacg acgggagaag ccgcgatttg
     4321 tttgcgtcct cacaacattg tgctgactgc cgtgcagtcc gaagcagaga cgcttgcgcg
     4381 ccaggattat aatgtgttct cgggtgtggt gcagcgcaga atatacttcg gcgagagtgc
     4441 ggactacacc atcgatctcg ctccgcatcc tttcacgctt cgggtcgtcg gctcgccgtc
     4501 taagctttat gacaagggcc aaaggatttt cgcactggcc cggcccgaac actgcgttgt
     4561 tgtaagtaac cattaagcga gcagagacaa tccaaagtgt cggttttctg ttctcttgat
     4621 cggattattt ccggctgtta tacgctacag tacgtcactt cggtgcatcg agtcctgcct
     4681 agcatcatcg tcggccggct actttgccaa gcgatagctt aaatgcattc gcacggatcg
     4741 gcttaaacct gaggtaaaat cgaggatcga ttctgagagt gaggaatacc aatacaatgc
     4801 ctgattatgt caaagagcat tacgactttc ccctgactca gaagagcgag aataagccgc
     4861 taaaaggcgt gcgcgtgatc gatgcgggca acatggttgc ggcgccgttt gccaccgtct
     4921 tgctcgccga ctttggcgcc gatgtgatta agatcgagca ccctaaatat ggcgatggcc
     4981 agcgcaagct cgaaccgatc atgaacggca ttccgctctg gtggaaggtg gtggcgcgca
     5041 acaagcgctg catcactctg gacctcggca agccggaagg cgcggaaatc ttcaagcaac
     5101 tgatcaaagg caaggacgca attgtcgaaa attaccggcc ggggaccttc gaaaagtggg
     5161 gaataggtcc ggatgtcatc cgccgtatcg atccgcgcat cattctgctg cgcatctccg
     5221 ggttcggcca gactgggccg tacaaaaatc gcgcgggatt cggtcgcgtc gcggaagcca
     5281 tgagtggctt gaccaacttg atcggcgagc cggacggccc gccgatgtcg cccggctatc
     5341 cgcttggcga tttgatcgcc gggatgttcg gatcgctatc gatcatgatg gcgctctatc
     5401 atcgcgatct ccgaggcggc gaagggcagg tgatcgatct tgcgcttttc gaagcggtgt
     5461 ttcgcttttt ggatttcgat ccgattcagt acgaccagat gaagacggtg cacatgcgca
     5521 ctgggaacag ggtcgcttac gttgcgccca gctcgatgtt taagaccaag gatggcaaat
     5581 atctgacctt ggcggcgagc acgcagaatg tctgggagcg acttgccgat gccatcgggc
     5641 gcaaggatct catcactgat cccaagttca tcgacaatcc cgcacgggta gaaaactcgg
     5701 tcgaatgcaa tggcatcgtc ggcgcatgga tcggacagca cacgcgcgac gaggtaatcg
     5761 agcaattcga taagttcggg gtggcctact cggctgtctt cgacatggaa gatgccttcc
     5821 gcgatctcca atatcgcgcc cgggcagcga tggttcgcgt gccggatccg gacctcggcg
     5881 aagctgtcgt gcagaacgtc gtgcccaagt tttctgcaac accgggttca gtcgactttc
     5941 tcggcgccaa aatgggcgcg cacaacgaag agatttacgg cagcgagctt ggcttatcca
     6001 aagaacgctt gagggaactt aaagaagcgg gggtaattta agctccgtaa tccgccgtga
     6061 taacagcatg gcgccatcgc tgttcgtcgg ttgatcgcgg taacgacgat tctgacaaga
     6121 gtttacgatg agcgacctga ggcaaaaact tcaacatttc ttaagagtga ttggtggtca
     6181 aattcttact atcgctcgcg cgggcttgac gaacacgatc gcatactcga cgaaaatgat
     6241 cgcgcttgtc ttgagtgcca gtctgcggca atgcgatgat ttccgctgcc ggttc
//
LOCUS       DASUTG010000119         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_166676, whole genome shotgun sequence.
ACCESSION   DASUTG010000119 DASUTG010000000
VERSION     DASUTG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166676"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1252)
                     /locus_tag="VF089_02625"
     CDS             complement(305..1252)
                     /locus_tag="VF089_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172881.1"
                     /translation="MLLSTTDVAAQPLTRVMSGYAATSGPHAVLWLAREAGLFEKNGL
                     RTDIAYIRSGSTMGQALVAGEIQLAQMGGPAALAAGVAGFDVTMVAVALNTTPIVIMG
                     KVSTMEELKGKAIGVTRYGSNTDISARFAIRKAGLQPEKDVALIQLEDYSGIMGGIQS
                     GRIAAGALADPFTDHAKKLGYKEIADIATLGLEFPFVGIVTKKTFIKQQPDVVQRFVR
                     AYTESIALYKNNRELAKKVTSKYTGIKDPEILNSTVDFYAPKLASVPYPTIGGIRFVL
                     DQVATRDPRAKNFAPETFMDLRFVKQLEDSGFIQGLSRK"
     gene            1376..>2315
                     /locus_tag="VF089_02630"
     CDS             1376..>2315
                     /locus_tag="VF089_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172882.1"
                     /translation="MKYGRQLLAGSLIALATLCQAAVTRAANINVVWTSEVGQFAPLW
                     ITKEAKLFEKYGNQVQLIFIQGAASAAAALSSDDAQVGMFSPQVVISTPALELVMFGR
                     LGNTMDNRIYARKGVKSLKEVKRIAISRFGSNADFAARYLLQREGLRPDTDVALLQVG
                     NQANRIAAVETANADAAMLTPPMTLQARKLGFSLIADASKLGIPYSSLFFVSRRPYLT
                     KNRADLVNFTKAMIEGIHYYKANKEFSMKVIGKYMKVQDREVIEENFREYDFPLKPYP
                     ATEFYDLPIQEVGKKDPKILKEDPERFADQSLVKELD"
BASE COUNT          547 a          667 c          582 g          519 t
ORIGIN      
        1 cgactcgtgg ccggaagctt tacgcgcgga ctggcaaaaa tggtttatgc tgctaaagat
       61 caacgctacg atcggcccaa tcgcaacgtt actgggcctg gcactcgtca ggcgttagac
      121 gcggtatgcg agcataacgg gcgctctata aattacgaac tcaccaaccg caggtcacga
      181 aggctttgac gggtagcgga attcttaagc ttcgtgctct tcgtgacctc cgtggtgaaa
      241 gttctatact atttacttcc ggctgatacc ttcaactgtc atatatcttc gtcaaatcgg
      301 ctcgctactt ccggctcaat ccctggatga acccgctgtc ctcgagttgc tttacaaacc
      361 gcagatccat gaaagtctca ggagcgaagt tcttggcgcg gggatcgcga gtcgcgacct
      421 gatccagtac aaatcgtatt ccgccaatcg tcggatacgg cacgctggct agcttgggcg
      481 cgtagaaatc aaccgtggag ttcaaaattt caggatcttt gatgccggtg tatttgctcg
      541 ttaccttctt cgccagttca cgattgtttt tatagagcgc gatcgattcg gtataggcgc
      601 gcacgaatcg ttggacaaca tcgggctgtt gcttgatgaa agttttcttg gtgacaatcc
      661 cgacgaacgg aaactccagg cccagcgtcg cgatgtcggc gatttcctta tagcccaact
      721 tcttggcatg atccgtaaac ggatcagcca gcgcgccggc ggcaatccgc ccggactgaa
      781 tgccgcccat aatccctgaa tagtcttcca actgaatcag agccacatct ttctccggtt
      841 gcagcccggc ttttctgatc gcgaagcgag cagagatatc ggtgttggaa ccgtagcgcg
      901 ttaccccgat cgctttgcct ttgagctctt ccatcgtaga aactttgccc atgatcacga
      961 tgggcgtagt attcagagcg acggcgacca tcgtcacgtc aaaaccagcg acacctgccg
     1021 cgagagcggc cggtccaccc atttgcgcta gttggatctc tccggcgacc aacgcctggc
     1081 ccatggtcga gccgctgcgt atgtaagcga tgtccgtgcg caaaccattt ttctcgaaca
     1141 gaccggcttc acgagccagc cagaggactg catgaggtcc cgacgtggca gcgtaacccg
     1201 acatcactcg agttaatggc tgcgctgcga catcagtcgt tgacagcagc acgaaaaagc
     1261 gatccagagt cggctcattt gcgtcatcct tacttgattc tcacgtcttt atcgcagtat
     1321 aggattaagc catcggcgtc aatcttgttg tcagacccta cagaaggaga tgcgaatgaa
     1381 atacggccgt cagcttttag ccgggagctt gatcgctctc gccacattgt gccaagcagc
     1441 cgttacccgt gcggcaaaca tcaacgtcgt ctggaccagc gaagtcggcc agttcgcgcc
     1501 gctatggatc accaaggaag ccaaactgtt tgagaaatat ggcaatcaag ttcaactcat
     1561 tttcatccaa ggcgcagcat cagccgctgc cgcgttgtct tccgacgacg ctcaagtcgg
     1621 catgttttcg cctcaggtag taatctcaac gcccgccttg gaactggtca tgttcggccg
     1681 gctcggcaat accatggata atcgcattta cgccaggaaa ggcgtcaaga gcctcaagga
     1741 agtcaaacgc attgccatca gccgtttcgg cagcaacgcc gatttcgccg cgcgctatct
     1801 tttgcagcgc gaaggactca gacccgacac cgatgtggcc ttactgcagg tcggcaatca
     1861 ggccaatcga attgccgccg tcgagaccgc taacgcggac gccgccatgc tcacgccgcc
     1921 gatgaccttg caagcgcgca agctcggatt ctccctgatc gccgatgcgt ccaagctcgg
     1981 cattccttac tcgagcctat tcttcgtttc acgccgcccc tacctgacaa agaatcgcgc
     2041 cgacttggtg aacttcacca aagccatgat cgaaggcatc cactactaca aggcgaacaa
     2101 agaattcagc atgaaagtca tcggcaaata catgaaagtc caagaccggg aagtgatcga
     2161 agaaaacttc cgcgaatatg atttcccttt gaaaccctac ccagccacag aattttatga
     2221 tttgccgatc caggaagtcg gcaagaagga tccgaagatc ctcaaggaag atccggagcg
     2281 tttcgccgac cagtcactgg tcaaagaact cgacg
//
LOCUS       DASUTG010000120         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_19089, whole genome shotgun sequence.
ACCESSION   DASUTG010000120 DASUTG010000000
VERSION     DASUTG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19089"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1288)
                     /locus_tag="VF089_02635"
     CDS             complement(320..1288)
                     /locus_tag="VF089_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6172883.1"
                     /translation="MASTKIIKGIIGACLTPFDDNDHIDYAALEKEMDFLLADCDAVT
                     IAAVEAAEYTMLTREERKELLKVGTQMVDKRVPVIIGCSSPSPREVIDLAEYAATIGG
                     DFVQVLMPLRPWGGQPTIAELMEFYTQVASASPLPVVCYHNPGPGADPPQDAFVKISE
                     IDNIRYFKESSRDITKISRLIEQIELAGRGHYFTTMQPLLVTLMLGGSGATMPPPGTR
                     IGAQIVKAFRANDLESARFWARCYALFPGKWAAYGLPPVMKSAMKHFGVDIGNPSRPY
                     APVSPRDHAQIGQFLRQVGLIGEAPPNPKALIDAANFLAQEDTFLR"
     gene            complement(1304..1957)
                     /locus_tag="VF089_02640"
     CDS             complement(1304..1957)
                     /locus_tag="VF089_02640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6172884.1"
                     /translation="MEMVDLSRIIYDGMPKIPALPDVHVQKFFSLDKGHPLNVTELSL
                     PCHAGTHVDAPIHIMPNGKSIEELPLDAFVGNGAIIAVRKSGGEEVTAKDLQASGVEV
                     KQGDIVMLYTGWDEKFDSSDYNLHPYLSVDAAEWMVEKGIKMFGIDCITVDLPTPLRP
                     KGFDFPVHKTLLSNNILIAENVTNLGPIVGKRCRIMAFPLRIKGSDAGHARIVAELN"
     gene            complement(1961..2722)
                     /locus_tag="VF089_02645"
     CDS             complement(1961..2722)
                     /locus_tag="VF089_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6172885.1"
                     /translation="MALSSAEFVKEIQQMVRKKHSKDHPIIGMIEEGQLDREQLKGFI
                     GQFYLFFPKPFPKPIAAMLSRCPDDPELEKMWIENVVEEGTGEITGTDSHKGLFIQFA
                     QSCGFTKEELDKVEPLPESQAFLDWRELLMYQRTWLELYACQGFCLEGTANERMTKIV
                     NGLTKHYGFKRDSETIRYWTLHMGVDEEHMKVGPLAVERYAITEPQQAQVRSSVQKTL
                     DQFWLAFDGIKRAFVDKDDLYAKWRNSDSKSNGRG"
BASE COUNT          670 a          756 c          642 g          690 t
ORIGIN      
        1 ctgccacagc cgccgcctaa tatcacgacc tgttttcgcc cattcaatcg agacatttat
       61 cgaaacctac tcagaaagtt ttttcctatg acgctgctga tatcgcatcg taaggccttt
      121 caagttccgc aaccgctgct ctcgccatag aagctgctct gctcggcctg cgccagtgag
      181 ctctttgccg aaagaagatg ttatacggag aaaccaaata acaaatttgt cggaaagttc
      241 tttaatccat tccatcacaa ctccaacgag accgtacgcg gtgcgagaag attgctttta
      301 gcgattatcc atcgtcaagc tagcgtagaa aggtatcttc ctgagcgaga aaattagcgg
      361 catctatgag ggctttcgga ttcggcgggg cttcaccaat caacccgacc tgccggagaa
      421 actggccaat ctgagcatga tcgcggggac ttaccggagc atagggccgt gaaggattgc
      481 cgatatccac accgaagtgc ttcatggcag atttcatcac cggcggcaaa ccgtaagcag
      541 cccactttcc agggaataat gcgtagcagc gcgcccaaaa ccgggcgctt tcaagatcgt
      601 ttgctcgaaa cgctttaaca atttgcgcgc cgatgcgggt tccaggcgga ggcatggtag
      661 caccggagcc gccaagcatt aaggtgacta gcagcggctg cattgtggtg aagtaatggc
      721 cgcggccggc aagctctatc tgctcaatca gcctcgaaat cttcgtgata tctcgcgagc
      781 tttccttgaa gtaccggatg ttatcaattt cgctgatctt gacaaacgcg tcctgcggcg
      841 gatcggcgcc aggcccgggg ttgtgataac aaaccacagg caagggactt gccgatgcca
      901 cctgagtata aaattccatc agctctgcga tcgtcggttg accgccccac ggacggagcg
      961 gcatcagcac ttgaacaaaa tcgccgccaa tggtggctgc gtactcggcc aagtcgatca
     1021 cttcgcgagg cgacgggcta gaacagccaa taatgaccgg cacgcgctta tcgaccatct
     1081 gcgtgcccac ttttaaaagc tcctttcgct cttcacgagt gagcatggtg tactcggcgg
     1141 cttccacagc ggcaatggtc accgcgtcac aatcggcgag aagaaaatcc atctccttct
     1201 ctaaggcggc ataatctata tggtcgttat cgtcgaaagg cgttagacaa gcgccgataa
     1261 tccctttgat tattttcgtt gaagccataa attttactta acgctaattt agctcggcga
     1321 caatccgagc atgtcccgca tcgctgcctt taatccggag agggaaagcc atgatccggc
     1381 atctcttacc aacgataggt ccaagatttg tcacgttctc ggcaattaag atgttgttac
     1441 ttagaagagt tttgtgcact gggaaatcaa atcccttggg cctaagcggc gtcggcagat
     1501 cgacggtgat gcaatcgata ccaaacatct ttatcccctt ctcaaccatc cattcggcag
     1561 catccactga caagtaagga tgaagattat agtcagaact atcgaacttc tcgtcccaac
     1621 ccgtatagag catcacgata tcgccctgtt tcacctcgac cccggacgct tgaagatcct
     1681 ttgctgtcac ttcttctccg ccgctcttac gtacagcgat gatcgcgccg ttgccgacaa
     1741 aagcatcaag cggaagttct tctatcgatt taccgtttgg cataatgtga attggcgcat
     1801 cgacgtgagt gccggcatga caaggcaatg acagctcggt cacattcaaa ggatgcccct
     1861 tatcgagact aaaaaacttc tgtacgtgta cgtccggcaa agccggaatt ttcggcatac
     1921 cgtcataaat aattcgcgaa agatctacca tctccatgga tcaacccctt ccatttgatt
     1981 tgctatctga atttctccac ttggcgtaaa ggtcatcttt atcgacgaac gctcgcttga
     2041 ttccatcaaa agcaagccag aattgatcca gagttttttg cacggagctc cgcacctgtg
     2101 cctgctgagg ttccgtgatc gcataccgct cgacagccag cggccccact ttcatatgtt
     2161 cttcgtcgac acccatatgg agtgtccaat atcgaatggt ctcagaatcc cgcttgaatc
     2221 cgtaatgttt cgtaagtcca ttgacaatct tcgtcatgcg ttcgtttgcc gtaccttcga
     2281 gacaaaaacc ctggcatgca tacagttcga gccaggtgcg ctggtacatc aaaagctcgc
     2341 gccaatcgag gaacgcctgg ctttccggta gcggctctac cttgtctaat tcttccttgg
     2401 taaagccgca cgattgagca aattgaatga aaagcccctt gtgactgtcc gtgccggtga
     2461 tctcccccgt cccttcttcg acaacgtttt cgatccacat tttttcgagt tccggatcgt
     2521 ccggacagcg gctgagcatc gccgcgatag gcttaggaaa aggttttggg aaaaacagat
     2581 aaaactgacc aatgaatcct ttaagctgct cgcgatccaa ttgtccttcc tcgatcatgc
     2641 ctatgatggg gtgatccttt gagtgctttt tacgcaccat ttgttggatt tccttgacaa
     2701 actccgcgct agaaagtgcc atcagccatt ctcctgttta tcggtccagt tctacgtc
//
LOCUS       DASUTG010000121         3807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1353519, whole genome shotgun sequence.
ACCESSION   DASUTG010000121 DASUTG010000000
VERSION     DASUTG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353519"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF089_02650"
     CDS             <1..1164
                     /locus_tag="VF089_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6172886.1"
                     /translation="LKVLEEGVILEGTVKNITGYGAFVDLGGIDGILHISDMSWGRIS
                     HPSELLQVGQKVNVVVLKFDSEKERISLGMKQLMPDPWHSVAEKFPIGMRVQGKVISI
                     MDYGAFVELESGIEGLIHISEMSWTRKIAHPSKILQVGQSVEVVILNVDPEHRRISLG
                     LKQVMPNPWEEAKQKYPVGSVVKGPVRNITDFGIFVGIEEGIDGLVHVSDLHWTKKVK
                     HPSELFKKGDIVEAKVLGVNVENERFSLGIKQLATDPWKVIAERFPVGTRVKGQVTSV
                     PEFGVFVRLDEGVEGLIHVSQLSTERVEKPSALYKVGDEVEAEVVNIDLTERKIGLSI
                     RALRKSEERQEMESYLSRDKQGGMFSFNTLLNEELRLDRDEEGQARNKSKGNS"
     gene            1188..2087
                     /gene="sppA"
                     /locus_tag="VF089_02655"
     CDS             1188..2087
                     /gene="sppA"
                     /locus_tag="VF089_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX6172887.1"
                     /translation="MARKHPILKGLGLLTGFVLVVFAAAFFYAYFTGGDAKALSLLAG
                     DGVGILQVEGTIDDSRSILTELRRFRDAPWIKAIVLRIDSPGGAVAPTQEIFDELQKV
                     RSKKPLIASMGGMATSGGYYIASACEKIVANPGTMTGSIGVIMQLNSVEELMKKIGVK
                     GQNIKSGVNKDIGSPFQSLSPEGRAILQSLVDNVHGQFVSAVATGRGMDEALVKKLAD
                     GRVYSGAQAKDLGLVDELGNLEYAVDLAAKRVGLEESPVVYYSRLEQERWWDRLFFSV
                     TGIQWSGNDRPGLRYEWSLSLLR"
     gene            2146..2457
                     /locus_tag="VF089_02660"
     CDS             2146..2457
                     /locus_tag="VF089_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX6172888.1"
                     /translation="MTKRDLIDEVNKRFPHLSRRDSEVIINAIFDSMVEALAQGDRIE
                     IRGFGSFVVKHRRARQGRNPKSGVLVSVAAKRVPFFKVGKELRFRVDGKPFEEEEDAT
                     T"
     gene            2454..2972
                     /locus_tag="VF089_02665"
     CDS             2454..2972
                     /locus_tag="VF089_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6172889.1"
                     /translation="MKQLWAPWRMVYVDQDSIETGCIFCNQSQGVEESRQALVLARSA
                     HSMVMLNKYPYNNGHLLLSPQQHERDLSKLSVSEHADLSEALRRAIDIVRGVLKPSGI
                     NLGMNLGKCAGAGVEDHLHWHIVPRWEGDTNFMPVIAETRVMPQHLLDSYDRLAPHFE
                     NFHHEVSQNARR"
     gene            3066..>3807
                     /locus_tag="VF089_02670"
     CDS             3066..>3807
                     /locus_tag="VF089_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6172890.1"
                     /translation="MNDAKTIGELKKSGVAVLPIREEMRKNLVHCLETGKRILPGIVG
                     YDETVIPEIENAILSGHHMVFLGERGQGKSRIIRGLMNLLDERIPIVQGCEINDNPYH
                     PICRSCRKKIQELGDNTPIQWIDRDTRYGEKLATPDVSIADLVGEIDPIKVAEGRYLA
                     DEETIHYGLVPRTNRGIFAINELPDLTEKVQVGLFNLMEEKDVQIKGYKIRLPLDVVI
                     VASANPEDYTSRGRIITPLKDRFDVQIRT"
BASE COUNT          945 a          893 c         1111 g          858 t
ORIGIN      
        1 ctaaaggtcc tagaggaagg tgtcattctg gaaggtaccg tgaaaaatat cacgggctat
       61 ggcgccttcg tcgatctcgg tggtatcgac ggtatcctgc acattagcga catgtcctgg
      121 ggacgtatca gccatccctc ggaacttctt caggtgggtc aaaaagtgaa cgtggttgtg
      181 ctgaagtttg attccgaaaa ggaacggatt tctctgggca tgaagcagtt gatgcccgat
      241 ccatggcaca gtgtggcgga aaaatttccc atcggaatgc gcgtgcaagg caaggttatc
      301 agcatcatgg actacggggc ttttgtcgag ctggaaagcg gcatcgaagg gttgattcat
      361 atttccgaga tgtcgtggac gcgaaagatt gcgcaccctt ccaagatcct ccaagtcggg
      421 cagtcggtcg aagtcgtgat tctcaacgtt gatccggaac accggcggat ttcgttaggt
      481 ttgaagcagg tgatgccgaa tccatgggag gaggcgaaac aaaaatatcc ggtcggcagc
      541 gtggttaagg gaccggtacg gaatatcact gatttcggca ttttcgtcgg cattgaggaa
      601 gggatcgacg gtttggtcca cgtgtcggat ttgcactgga cgaagaaggt caaacatcca
      661 tccgaactat ttaagaaggg cgatatagtt gaagcgaaag tattgggggt caacgtcgaa
      721 aacgagagat tctctctagg catcaagcaa ctggccaccg acccgtggaa agtcatcgcc
      781 gaacgtttcc ccgtggggac cagggtgaag gggcaggtca cgagtgtccc tgaattcggc
      841 gtgttcgttc gtctagatga aggggtggaa ggtttaatcc acgtctcgca gctgagcact
      901 gaacgggtcg agaagccttc ggccttgtat aaagttggag atgaggtgga ggccgaggtc
      961 gtcaacatcg atctgacgga aaggaagatc ggcctaagca tccgcgcctt gcgcaagagc
     1021 gaagagcgcc aggagatgga aagctatctc agccgcgaca aacaaggcgg aatgttttca
     1081 ttcaacacct tgctgaatga agagctgcga cttgaccggg atgaagaggg gcaggcacgc
     1141 aacaagagca aaggtaactc ataagaatta ggagcacagt cgtcaaaatg gcaagaaagc
     1201 atccgatcct caaaggtttg ggtctgctta cgggttttgt actggtggtc tttgcggccg
     1261 ccttctttta cgcctatttc accggtgggg acgccaaggc tttgtcgctt ctcgctggag
     1321 acggtgtcgg cattctgcag gtcgaaggca ctatcgacga ttcacggagt atcttgacgg
     1381 agctccggcg ctttcgtgat gcgccgtgga tcaaagccat cgtgctgcgc atcgattctc
     1441 cgggcggtgc ggtggcgccg acgcaagaaa ttttcgacga attgcaaaag gtgcgaagca
     1501 agaaaccgtt gatcgcttcc atgggtggaa tggcaacctc gggaggctac tacattgcca
     1561 gtgcctgtga gaaaattgtc gctaacccgg gaaccatgac cggctcaatc ggcgtgatca
     1621 tgcagcttaa tagcgtcgaa gagctgatga agaaaatcgg ggtaaaggga cagaatatta
     1681 aaagcggcgt caataaggat atcggttcac cctttcaatc gctgtctcct gaagggcgag
     1741 cgatcttgca gtcgcttgtg gataacgtcc acggccaatt cgtcagcgcc gtggctacgg
     1801 gccgaggcat ggacgaggct ctcgtcaaga agcttgccga tggccgggtc tattccggag
     1861 ctcaggcgaa agatctcgga ttggtggatg aattgggtaa tttggagtat gcagtggatc
     1921 tggcggcgaa gcgggtcgga ctagaagaaa gcccggtcgt ttactactcg cggctggagc
     1981 aggaacgctg gtgggacaga ttattttttt ccgtgaccgg tattcagtgg tccggcaacg
     2041 atagaccagg gcttcgctac gagtggtcgt tgtctctgct acgttgaatc atgcagtcaa
     2101 tgcttttccg ggccaagaga ttttgttacg tcgcgaggga gcgttatgac gaaaagggac
     2161 ttgatagacg aggtcaataa aagatttcca catctttcga gaagggattc ggaagtcatt
     2221 atcaacgcca tcttcgactc gatggtcgaa gctctcgccc aaggtgatcg catagagatc
     2281 cgcggctttg gcagttttgt cgtaaaacat cggcgggctc gtcagggccg taatcccaag
     2341 agcggggtac tggtctcggt ggcggcaaaa cgagttccgt ttttcaaagt tggcaaggaa
     2401 ttgcgcttta gagttgatgg aaaacctttc gaggaagaag aggatgctac tacatgaaac
     2461 agctctgggc tccttggaga atggtttatg tcgaccagga ctccattgag acaggctgca
     2521 tcttctgtaa tcaaagccag ggggtggaag aaagccgtca agcacttgtt ctggcgcgca
     2581 gtgcgcatag catggtgatg ctcaacaagt acccctataa taatggccac cttcttctat
     2641 ctcctcagca gcacgagagg gatctttcca aactctcggt ctccgaacat gccgacctta
     2701 gcgaagcgct aagacgggcc atcgatatcg ttcggggtgt cctgaagccc tccggaataa
     2761 atctcggtat gaacctggga aaatgcgccg gcgcaggagt ggaggatcat cttcattggc
     2821 atatcgtgcc gcgctgggaa ggcgacacca acttcatgcc ggtgattgcc gagacccggg
     2881 tgatgccgca gcatctcttg gacagttacg atcgcctggc gccgcatttt gagaattttc
     2941 atcatgaggt gtcccaaaac gcccgccggt agtgtaggct gctatcaggg acaactcatc
     3001 caatctgtaa agaagcactt cgactttaaa caaacattac tcgttataga gggaggccgg
     3061 tcaacgtgaa tgacgcgaaa acgatagggg aactcaagaa aagcggtgtc gccgttttgc
     3121 cgattcgcga ggagatgcgc aagaatctcg tccattgcct cgaaaccgga aaacggattc
     3181 tgcctggaat tgtcggctac gatgagacgg ttatccccga aatagaaaac gccatcttgt
     3241 cggggcacca catggtcttc ctcggcgaac gcggacaggg taagtcgcgc attattcgcg
     3301 gcctgatgaa tctgcttgat gaacgcattc caattgttca gggctgcgaa atcaacgaca
     3361 atccgtacca tccgatttgc cgctcctgcc gcaaaaaaat tcaagaactg ggcgataaca
     3421 cgccgatcca gtggatcgac cgcgacaccc gatatggtga gaagttggca actccggatg
     3481 tttcgatcgc cgatttggtg ggtgaaatcg atccgattaa agtcgccgaa ggacgttatc
     3541 ttgccgacga ggaaacgatt cattacggtc ttgtcccgcg caccaaccgc ggtatcttcg
     3601 cgatcaatga attgcccgac cttactgaga aagtgcaggt gggtttgttc aacttgatgg
     3661 aagaaaaaga cgttcaaata aaaggttaca aaatccggtt gccgttggat gtcgtgatcg
     3721 tcgccagcgc caacccggag gattatacca gccgcggccg cattattacg ccattgaaag
     3781 accgctttga cgtgcagatt cgcaccc
//
LOCUS       DASUTG010000122         8182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1501553, whole genome shotgun sequence.
ACCESSION   DASUTG010000122 DASUTG010000000
VERSION     DASUTG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501553"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VF089_02675"
     CDS             <1..636
                     /locus_tag="VF089_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6172891.1"
                     /translation="HILDHEGVTDGYGHISVRVPGADAFITIANVSPGCVTEDRLIMQ
                     DLAGKYLGGAETPPNEWPIHACILKARPDVESVAHTHSKWSTIFSVLPIKLKPMHHYG
                     KFLSADGPPVYEGIGLVRTVARGNDLAQALGDGPVVLMRAHGDTVVGESVEQVIERTT
                     RLAMLGEWNHLALLHGEPNYLTADELEVYNADQRFPMRGWEYFISRLNGRK"
     gene            740..1432
                     /locus_tag="VF089_02680"
     CDS             740..1432
                     /locus_tag="VF089_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6172892.1"
                     /translation="MEKQNGTVSSAEFVHQLKKDIQAFPKIRIKHPFLKAVCAGTASM
                     EQIRAWAIQDYQFRAAVPRIAMLRYLACTDPEIARKLWGVVEEETRGLDTGSAGHNEL
                     FIRFADSIGLTRDQLENTELRPATAAHLYYVELIIHTLPWFVVMAIQIGAEGTFGPAA
                     AALGHGFIKQYNMKPDDVRFFTVHSEADEDHGNLAEEIAVRYITSPHLQELTRKHNFR
                     RMELLYDIWSVD"
     gene            1577..2590
                     /locus_tag="VF089_02685"
     CDS             1577..2590
                     /locus_tag="VF089_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172893.1"
                     /translation="MAKLTLSFISAFNERVEPLMNGTVQAEGIELIPTYSHPAETFWR
                     QLKFHEFEVAEMSMSSFLIARSQDVDMVALPVFPSRRLFQTEISYHVDSGVSKPGDLA
                     GRRLGVAEYQQTAALWIRGILEHDFGVSQYKIHWYMERSEEMSHGGATGFKPPAGISF
                     NRIAPNKSLASTLLEHELDAAHIASPWVLRANALDRSSRITASGADWSKIKPLFPDRM
                     AEAQRFQKQHGFLPVNHTYIIRGDIYRKHPWVAFNLYSGFVRAKALAREKLVESIPSA
                     LFFGPEYLKRTQDIIGEDPFPYGIKANEAMLETITTYSYEQGLTPKKMNYKELFAVNT
                     LEL"
     gene            2657..3670
                     /locus_tag="VF089_02690"
     CDS             2657..3670
                     /locus_tag="VF089_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172894.1"
                     /translation="MPRLTLSFISAFNERVDPLMNGTVKPEGIDIVPTYSHPSETFWR
                     QLKFQEFDIAEMSMSSYLIARARGFDMIALPVFPSRRLFHTELSYHTDSGIKEPGDLI
                     GKRLGVGEYQQTAALWTRGILEHEFGVSQFKVHWYMERTEELSHGGATGFTPPPGISF
                     QRIPPEKSMASMLVDHELDVAAISSPWKNMPNVIGRSHRIKGSDGDWSKVKPLFPDRM
                     SEGARLFQKFGFLPVNHAYIIRGDIYNKYPWVAFNLYSGFLKAKQYFNEKLLERIPSA
                     LFFGPEYLATTRQMFGDDPYPYGVKANRKMLATLIDYSHEQGLTSKKLKIEELFAPAT
                     LDL"
     gene            4327..4512
                     /locus_tag="VF089_02695"
     CDS             4327..4512
                     /locus_tag="VF089_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172895.1"
                     /translation="MGSSHLLWVAQDRGFLAKYGIEANLVFISSGSLNVQGWSVILFS
                     SPTAVPPHWKRGCAARS"
     gene            4779..5081
                     /locus_tag="VF089_02700"
     CDS             4779..5081
                     /locus_tag="VF089_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172896.1"
                     /translation="MIVRRSLVDENPWLVSSLLDAVNQAEERCRKSYDYAKRMAFPTA
                     VLILEEEEEIFGENPRSHGLTAQNQVVLDKFVHYAHEQGYISERPPISDLFAPVGN"
     gene            complement(5095..5871)
                     /locus_tag="VF089_02705"
     CDS             complement(5095..5871)
                     /locus_tag="VF089_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6172897.1"
                     /translation="MREGKFTLLEKRGPVAKITINRPDKRNALSRDTIREMLGALEEI
                     RNDDSIAVVLTSGAGDVAYCAGRDLSEFPTEGGAAVEPERRAQPRAYHVAEAIRTFPK
                     VTIAVVNGFCLGAGITILLPHDLAIASDRAQFGLPEIKRGFLPYPIIATMFKTLIPTK
                     LAFELILTGENWDATKSMEAGLINRVVPHEQLYQAAWQWGEEIGRFDKTTLKYCKMAA
                     HASMEANSVPLAAEIAWLMQEEHSRVNPKAYAGTKQFHKK"
     gene            5987..6835
                     /locus_tag="VF089_02710"
     CDS             5987..6835
                     /locus_tag="VF089_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6172898.1"
                     /translation="MESALKQISRSLFAVIGPEGSTNFAIVKASDNSAALIDADIRRI
                     DEIEEALQLTGCSKVSYLIDTHEHFDHTSANFYFAQRGIPILASAGCAAAMRDEGGSD
                     FERMMKPVPELYERFPGLCLTLPDIVFRDSKTLVLPGATLHLRYCAENGHSHSKGDTT
                     IFFEEEEILIAGDLLYTEVHPVTFFGNIPNWLTALKPLCESHYKQLLPGHGPSVEGEQ
                     AGRAYFKKMYDYLEEFHGRMVEVKAGRQNAQDVAQHMLSGAYASFGKTRMVQRNVNHF
                     LTGRWF"
     gene            6836..7975
                     /locus_tag="VF089_02715"
     CDS             6836..7975
                     /locus_tag="VF089_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172899.1"
                     /translation="MEPTILQNDHRSPRHPVPSVSCQAQRDILPVPCGFREEGSLISS
                     ELALWLLTRQRLTLLLIAISAVLWFSASQVRAAEKIRVAYPAVAPGLTPSWITAEAGI
                     WRKYGLDVETILVSGGARAVPALISNSVQFLMGSDTGVTTALLQGLPVVRLGVTTNTL
                     GSSLLTQHTIQSVGDLKGKTLGISRGRDASYIRLAKLLRDHGLNPNEDVKFLPIGGGE
                     GGRLSALKAGVIQGTMLFPPLDFIASNEGLKVLEKFDVATPGGGINTTAALLKQNRTM
                     VINFLKGYMEGIQYMAQHKDHSLKILQRYFQNSDTTAMGYLYDETTRRLEKDLRPSQE
                     SIRFHLELAALDDPRAAKLGEKDFWDASVVEEIRRSGFIDQLYKK"
     gene            7986..>8182
                     /locus_tag="VF089_02720"
     CDS             7986..>8182
                     /locus_tag="VF089_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172900.1"
                     /translation="MGYYPSLREYVRTLENREKLFRIQREVCRETELHPLVRWQFRGL
                     PENERRAFLFEKVASVTGRKY"
BASE COUNT         1921 a         2312 c         2170 g         1779 t
ORIGIN      
        1 cacattctcg atcatgaagg cgtgaccgac ggctacggcc acatcagcgt acgcgttccc
       61 ggcgcagatg cgtttatcac catcgccaac gtcagccccg gctgcgttac tgaagatcgg
      121 ctgatcatgc aagacttggc cggcaaatat ctcggcggtg cagagacgcc gcccaatgaa
      181 tggccgatac atgcgtgcat tttaaaagcg cgtccagatg tcgagagcgt tgcgcataca
      241 cattcgaagt ggagcaccat ttttagcgtg ctgccgatca agctcaaacc catgcaccac
      301 tatggtaagt ttttatccgc cgatgggccg ccggtatatg agggcatcgg cttggtccgt
      361 actgtggctc gaggcaatga tctagcccaa gccttgggcg acggcccggt agtgctaatg
      421 cgcgcgcacg gggataccgt ggttggcgag tccgtcgaac aggtaatcga acgcaccacc
      481 cgccttgcaa tgcttggcga atggaatcat ctggcgctgc tccatggtga accgaactat
      541 ttgaccgcgg acgaattgga ggtgtacaac gccgatcagc gtttccccat gcgcggctgg
      601 gagtatttta ttagccggct gaacggaaga aagtgatcgc catctagacg aagtatcgcg
      661 gcaagatacc cagcagatta tcttgagcgc tcaatggaat gttgatacca aaccagattc
      721 agatcgggaa ggaaatgaaa tggaaaaaca gaacggcaca gtttcttcgg cagagttcgt
      781 tcaccaactc aagaaagaca ttcaggcttt tcccaaaatc cgcatcaagc atccgttctt
      841 gaaagccgtc tgcgccggca cagcgagcat ggagcagatt cgcgcctggg cgatccagga
      901 ttatcaattc cgcgccgcgg tgccgcgcat cgccatgctg cgctacttgg cctgcaccga
      961 tcctgaaatc gcgcgcaagc tctggggggt tgtcgaggaa gagacccgcg gtctcgacac
     1021 cgggagcgcc ggtcacaatg aactatttat tagatttgct gattcgatcg gccttacgcg
     1081 cgaccaattg gagaacaccg aactgcgacc ggcaaccgcc gcccatctct actatgtcga
     1141 gctgatcatt cacacattgc cgtggtttgt cgtcatggcc attcagatcg gcgcggaagg
     1201 aaccttcggc ccggctgcgg cagcgctcgg tcacggcttc atcaaacaat acaatatgaa
     1261 gcccgacgat gttcggttct tcactgtcca ctcggaagcc gacgaggatc acggaaatct
     1321 cgcagaagaa atcgcggtgc gctacatcac ctccccgcat ctgcaggaac tgacgcgcaa
     1381 gcacaatttc cggcgcatgg aactcctcta cgatatctgg agcgttgact aggtaaatag
     1441 ttaccggctg ctcaaaagtt tccagatccg aggcgcgcga aaacgccgag tggaggcgtc
     1501 ctcacattac gttgcagcga ggcgattgag cgcaaggaag catagggtct tttcaccgcc
     1561 ctggtaggag aacacgatgg ccaagctcac gttgagtttc atctcggcgt tcaatgaacg
     1621 agtcgagccg ctcatgaacg gcacagtgca agcggaaggc atcgaattga ttccgaccta
     1681 ctcccaccct gccgagacat tctggaggca gcttaagttt catgaattcg aggtagcaga
     1741 aatgtcgatg tcgtcctttc taatcgcccg ctcgcaggac gtcgacatgg ttgcgctgcc
     1801 cgtgtttccg agccgccgct tgtttcaaac tgagatctcc taccatgtcg attccggcgt
     1861 gagcaagccc ggcgacctgg cgggaaggcg attgggagtt gccgagtatc aacaaacggc
     1921 cgccctttgg atacgcggca ttctcgagca cgacttcggg gtatcgcagt acaaaattca
     1981 ctggtacatg gagcgaagcg aggagatgag ccatggcggc gccaccggct tcaagcctcc
     2041 agcgggaatt tctttcaatc gcattgcccc gaataaaagt ctggcctcga ccctgctcga
     2101 acacgagttg gatgcggcgc atatcgccag cccttgggtg ttgcgagcca acgctctcga
     2161 ccgctccagc cgcattaccg ccagcggcgc agattggagc aagatcaaac cgctttttcc
     2221 ggatcgcatg gccgaggccc aacgttttca gaagcaacac ggctttctac cggtcaatca
     2281 cacttacatc atccgcggcg atatctatcg aaaacatccc tgggtcgctt tcaatctgta
     2341 tagcggcttt gtaagagcta aggcgcttgc gcgtgagaag ctggtggaaa gcattccttc
     2401 ggcgttgttc ttcgggccgg agtatctaaa gaggactcag gacatcatcg gcgaagatcc
     2461 atttccctat ggcatcaaag ccaacgaggc aatgctcgaa acgatcacga catattccta
     2521 cgagcagggg ttgacgccca aaaagatgaa ttacaaggag ctgttcgcgg tcaacacgct
     2581 cgagttatga gcctcggcgt ccgctgcagc ggatctggga gccgttaaat cacaatcctt
     2641 tcaccgggag acaagaatgc ctagactgac tctcagtttc atttccgctt tcaacgaacg
     2701 ggtcgatcca ctgatgaatg gaacagtaaa accggagggg atcgatattg ttccaaccta
     2761 ttcgcatccg tcggaaactt tttggcggca attgaagttc caggagttcg acattgctga
     2821 aatgtccatg tcgtcgtatt tgattgcacg ggcgcgcggt ttcgatatga tcgcgttgcc
     2881 ggtatttccc agccgccggc tcttccacac cgaactctcc taccatactg actcgggcat
     2941 caaggaacca ggcgatctca tcggcaaacg actgggtgtc ggcgagtacc aacaaaccgc
     3001 ggccctatgg acacgcggca tactcgaaca cgagttcggc gtgtcacagt tcaaagttca
     3061 ttggtatatg gagcgaactg aggagttgag tcatggcggc gccaccggtt ttacgcctcc
     3121 gccggggatc tcattccagc gaattccgcc ggaaaagagc atggcttcaa tgctggtcga
     3181 tcatgaactg gatgtggcgg cgatctcgag cccgtggaaa aacatgccga acgtcatcgg
     3241 ccgctcccat cgaattaagg gaagcgacgg cgactggagc aaagtgaagc cgctattccc
     3301 ggatcgcatg agcgaaggcg caaggctctt tcagaaattc ggatttctac cggtaaatca
     3361 tgcctacatt atccgcggtg atatctataa taagtatccc tgggtggcat ttaatcttta
     3421 ttccggcttc ctcaaagcca agcagtactt caacgaaaaa ctgcttgagc gaattccaag
     3481 cgctctgttc tttggcccag agtatctggc cacgacccgg cagatgttcg gagacgaccc
     3541 ctacccctat ggcgtcaaag ccaatcgtaa gatgctggcc acgctgattg actattctca
     3601 cgaacaagga ctgacgtcga aaaaattgaa aatcgaagag ctgttcgcgc ccgccacgct
     3661 tgacctataa aacagtgcct cgttggttgc gccacgcaca gccaacgagg cacctagcgt
     3721 gtggataatt atctttgcta tgcgatcagg tacagccgcg cacgatgaag aagagcaaca
     3781 gtattggtac ggggatgcct agcagccata aaagtatcca accaatggca ccattttgat
     3841 tgttaaaact attcatagtc cctcctttta actacctgcc agacctgttt agtctgacgt
     3901 gtaacaagct tgactcctac ttccgcaagg catgtgccag ccgtgtctac gccattcaag
     3961 tttagaaact agtagcatcg cgtctttacc gtcccacgac tgaaacatcg tggctcaaat
     4021 gcgacaccgg gcagttgatg caaacgtaca ttcgcaagag ccgttacaaa agcgacatag
     4081 agcactcggc atacagcgaa gagctcgcag gagcagcgtt caggatgtag gaagaggaga
     4141 gcatcgacgg aataatgtaa tctccgtcgt gctcgtaacg ctgcttgact ccccccaccg
     4201 cttcacagta ttcaccgggc tgtgaacatt tgttggtatc aacgatagtc ttgttactgg
     4261 cgctttgttt ctttgggccg tcgctcggcg cggccggtcg cgttaaggcg tcatacgcgg
     4321 ccgtgaatgg gatccagtca cctactctgg gttgctcagg accggggatt cttggccaag
     4381 tacgggatcg aagccaatct ggtgttcatc agtagcggct cattgaacgt tcaaggctgg
     4441 tcggtaatac tgttcagttc gccgacggcg gtcccgccgc attggaagcg cggctgcgcg
     4501 gcccgaagtt agtaaccatt ggaatccgct taccttgctc gcgtcgaatc tggtgagcca
     4561 tccggataac aagagcattg ccgatctaag gaaaagtagg cgccgtttct cgctttgcat
     4621 cgccttcgca tcaaggactg cgctatctgt tccgcaaaaa cgggcgatcg gttgaaccaa
     4681 gaaaagtttt tagcaagcct ggataaataa tatcaccgtc cttccatccc aaaaaatggc
     4741 gcctacgcaa aaccaaaatt tttccgatca ctcacacctt gatcgtacgc cgatcgctcg
     4801 tcgacgaaaa tccgtggctc gtctcgagtc ttctcgatgc cgttaaccaa gcggaggaac
     4861 gctgccggaa atcctacgac tatgccaagc gcatggcgtt ccccaccgcg gtccttatct
     4921 tggaagaaga ggaggaaatt tttggcgaga atccccggtc gcacgggtta accgcgcaga
     4981 atcaagtcgt gctcgacaag ttcgttcact acgcacacga gcagggttat atttccgaac
     5041 gcccgcccat tagtgatttg ttcgcgccgg tgggtaattg aacggcaaca gcacttattt
     5101 cttatgaaac tgcttggtcc cggcataagc cttgggattc actctgctgt gttcttcctg
     5161 catcaaccaa gcgatctctg ccgccagtgg cacgctgtta gcctccatgg aagcatgcgc
     5221 tgccatttta cagtatttta aagttgtctt atcgaatcgg ccgatctctt cgccccattg
     5281 ccatgccgct tgatagagct gctcatgcgg cactacgcga ttgatcaatc ccgcttccat
     5341 cgattttgtc gcgtcccaat tctcgccagt gagaatcaat tcaaaagcca gttttgttgg
     5401 aattagggtt ttgaacatcg ttgctatgat cggataaggc aggaagccgc gcttgatctc
     5461 cggcaagccg aactgcgctc ggtccgaagc gatagcaagg tcatgcggca acagaatcgt
     5521 aataccggcg cccaggcaaa agccgtttac gacggcaatc gtgactttcg gaaaggtcct
     5581 aatcgcctcc gccacatgat acgcacgcgg ctgggcccgg cgctccggtt cgactgcggc
     5641 gccgccctcg gtggggaact cggacaggtc ccggccggcg cagtaagcaa cgtccccggc
     5701 gcccgaggtt aaaaccactg caatggaatc gtcgttacgg atttcttcaa gagcaccgag
     5761 catctctcgg atcgtgtccc ggctcaaggc atttctctta tccggccgat tgatcgtaat
     5821 tttcgcaacc gggccgcgct tttccaacaa agtaaatttg ccctctctca tgaacaagcc
     5881 ctttcttaat tttgaaattt catacgcgaa taacaccgcc ggtgcttgcg gtcaaccgct
     5941 gccgacgctt gaaaccacgc ggcgactcgg ataagtcgag ggatctatgg agtctgcact
     6001 gaaacaaata agtcgatctc tattcgcggt gatcggcccg gaaggctcga ccaacttcgc
     6061 tatcgtcaaa gcaagcgaca acagcgccgc gctcatcgac gccgacatcc gccgcatcga
     6121 cgagatcgaa gaagcgctgc agttgaccgg ctgttctaaa gtcagctatc tgatcgacac
     6181 ccacgagcac ttcgaccaca cctcggcgaa tttctatttt gcgcagcgag gcatacccat
     6241 cctcgccagc gcgggctgcg ccgctgccat gcgcgacgag ggcggatcgg atttcgagcg
     6301 catgatgaag ccggtgcccg agctttacga gcgcttccct ggcctctgct tgaccttgcc
     6361 cgacattgtg ttccgcgact caaaaacact cgtgttgcct ggcgcgaccc tccatctcag
     6421 atattgcgcc gagaacggcc acagtcacag caaaggcgat acgacgattt tttttgaaga
     6481 ggaagaaatt ctcattgccg gcgacctgct ctatacggaa gttcacccgg tgacgttctt
     6541 tggcaatatt cccaattggc tgaccgcctt gaagccactc tgcgaaagcc attataagca
     6601 actcctaccc ggccatgggc cgtcggtgga gggcgagcag gcgggcagag cctatttcaa
     6661 aaagatgtac gattacctgg aggaatttca cggccgcatg gtcgaagtca aagccggccg
     6721 tcaaaacgct caagacgtgg cccaacacat gctgagcgga gcctacgcat ctttcggcaa
     6781 gacgcgcatg gtgcaaagaa acgttaatca ttttctcacg ggaaggtggt tttagatgga
     6841 accaaccatt cttcagaatg accatcgctc tcctcggcat ccggtcccat cggtctcatg
     6901 ccaagcacag cgagatatcc ttccggtgcc gtgtggtttc agagaagaag gatctctcat
     6961 ttcatccgaa ctggcactgt ggttgcttac tcgacagcgt ctcacactcc ttctcatcgc
     7021 gatttcggcg gttttatggt tcagcgcaag ccaggtccgc gccgccgaaa agatccgagt
     7081 cgcctatcca gcggtcgctc ccggcctgac gccctcgtgg ataaccgccg aggccgggat
     7141 ctggcggaaa tatggcttgg acgtggaaac cattctcgtc agcggcggtg cgcgcgccgt
     7201 cccggcgctg atcagcaaca gcgttcagtt tttgatggga tccgataccg gcgtcaccac
     7261 cgcgcttttg caaggtctcc ccgtggtgcg actgggcgtg acgacgaaca cgctcggctc
     7321 gtccctgctg acccaacaca ctatccagtc ggtgggcgac ttgaaaggaa aaacgctcgg
     7381 catctcgcgc gggcgcgatg ccagttacat acggctcgcc aagctgctgc gcgaccatgg
     7441 tctcaacccg aacgaggacg ttaagttctt accgatcggc ggcggagagg gcggccgatt
     7501 gtccgctttg aaggcgggag tcatccaggg aacgatgctt ttcccgccgt tagatttcat
     7561 cgccagtaac gagggattga aagtgctcga gaagttcgac gtggcaaccc cggggggagg
     7621 aatcaacacc accgcagcgt tgctcaaaca gaaccggacc atggtgatca acttcctcaa
     7681 aggctacatg gaaggcattc aatacatggc tcagcacaag gaccatagcc tgaaaatttt
     7741 gcaaagatat tttcagaact ccgacacgac ggcgatgggc tatctttacg acgagaccac
     7801 ccgccgtctc gagaaggacc tccgccccag tcaagagtcg atccgcttcc acctggaact
     7861 ggccgccctc gacgaccccc gggccgccaa gctcggcgaa aaagattttt gggacgcgag
     7921 cgtcgtcgaa gagattcgcc gctccggatt tatcgaccag ctctataaga aataggagat
     7981 accgcgtggg ttattacccg agtctcagag aatacgttcg gacattggaa aaccgcgaga
     8041 aactttttcg catccagcgt gaagtatgtc gcgagaccga gctccacccc ctggtacgct
     8101 ggcaattccg cggcctcccg gaaaacgagc ggcgcgcgtt tctatttgag aaagttgcga
     8161 gcgtcaccgg ccgcaaatac aa
//
LOCUS       DASUTG010000123         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1056831, whole genome shotgun sequence.
ACCESSION   DASUTG010000123 DASUTG010000000
VERSION     DASUTG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056831"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..764)
                     /locus_tag="VF089_02725"
     CDS             complement(318..764)
                     /locus_tag="VF089_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6172901.1"
                     /translation="MKLAVRFCAIALLLFAPVVLFAIDENPAVKVTQLLKTTSGWDGK
                     QIVYPEGQAELTALLIEIAPGESTGWHQHPVPSFAFLLDGILEITLRDGRVKRMQPGE
                     ALSEVTGAVHIGRVLSRTPVKLVVFYAGSVGKALTITQPDEQKTSQ"
     gene            1131..2765
                     /locus_tag="VF089_02730"
     CDS             1131..2765
                     /locus_tag="VF089_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6172902.1"
                     /translation="MAEEILVVHGGKLIDGTGRPPIDNSTILIRGRKFTAVGTDGEVA
                     VPAGAQVIDVTGKTVLPGFIDGHGHLEDFHGEIYLHLGITTCANIELYQDGPWTLAQK
                     QGTDLGKIRGPRIWMSGRAIGGVSTGHDAFGSRTFRDNIIVTTADEVRRAVQRKKELG
                     CDILKVNEFLSLDLVKVAVEEAHRLDMPVAAHSWDVVGYCDAGVDAIEHIWSVGYSSI
                     PYAPARRKLAEDRLGGVIDQELAGAYYQTENFDEVIGAMVQHQVAWTPTIAKWLRPLS
                     PSANRFRERENQILNDPEADLPAAVRAVTENSYDKLLKRYTPEQRQRARIGYDKANEF
                     IRRFVQAGGILKEGSDPPRGMAALLMHQALVMDVEAGVSPMTAIQAATLNVARTFGRD
                     KDYGSVEPGKVADLSIVDGDPLKDIWMTQNVKMVVMDGKVIDTGFKRYKNPIPSFYSF
                     QSLPLDLEIAPLFLTEGSGPTVLRVRGAGGMWPFHQVMLNGEPLPTSFVSKDELKAII
                     PPEAIAKAGTYIVTLKCEGEPFPESHRAHLVVGFKP"
     gene            3014..3841
                     /locus_tag="VF089_02735"
     CDS             3014..3841
                     /locus_tag="VF089_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6172903.1"
                     /translation="MELTIETNLRLNDGNLIPQLGLGVWQIGPAKTSDAVLAALEANY
                     RHIDTAAAYGNEESVGAAIMTSGIPRERIFVTTKLWNSDHGNPERALDTSLRKLKMDY
                     VDLYLIHFPVRQRRQSWRALEGLRAKGKTRSIGVSNFTIAHLTELLAESETVPAVNQV
                     EFHPYLYQQELLAFCQAKGIVIEAYSPLTHGERLKDPKLVAVARKYSSSKPKSTAQIL
                     IRWALQHGLVVIPKSSNRRRIVENADVFEFEISEDDMRLLDSFNENLRTCWDPSDTP"
     gene            complement(3968..>4645)
                     /locus_tag="VF089_02740"
     CDS             complement(3968..>4645)
                     /locus_tag="VF089_02740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6172904.1"
                     /translation="SRRWDFAVMSIGKERMRADGKSMDQRVSVSVSRRTARSLRTVLC
                     GIILAASLSGIGCNEQPTSATTSLREPDVRYEPTSPDVMQVMFRLANVKSGEVVYDLG
                     CGDGRIVITAVREFGARGICVDIDPQRIAESQENARAAGVTENIRFLNQDLFETDVSD
                     ASVVMLFLSPELNLKLRPKLLRELKPGARIVSHWHDMGDWKPQKVVRVRSSGRERSVY
                     LWTIPPR"
BASE COUNT         1035 a         1238 c         1323 g         1049 t
ORIGIN      
        1 taacaggact ctgtggccaa cagtggtgca atgaggcgcg actgtagatt tttggacgcg
       61 aagttgtcaa gcagaaattc gtgcgaaaaa agagattttt ctctgctgat tcaacttcat
      121 ttgttcatac ttctgtggat agtcttttta acaatagatt gccgctggaa aactaagatt
      181 atccacagct gtggataatc tttcggataa gccgaagatt tttttctcgg ttttccggga
      241 tgcccggtgc acaatcgttt tacgagcagc agtgatacct gggggatctg acagcaaacc
      301 tgcggtgctg ggtggtgtca ttgagacgtc ttttgttcgt ctggctgagt gattgtcaac
      361 gccttgccca ctgaaccggc atagaatacg acgagtttca ccggagtcct gctcagcacc
      421 cgaccgatgt gcaccgcgcc ggtcacttca gacaacgctt ctcccggttg catgcgctta
      481 accctgccgt ccctgagagt gatttccaga atcccatcaa gtaggaaggc gaatgaaggc
      541 accggatgct ggtgccagcc ggtgctttct cccggagcaa tctcgatgag cagtgccgtc
      601 agttccgcct gcccttccgg atagacaatt tgtttgccgt cccagccgct ggttgtcttg
      661 agaagctggg taaccttgac cgcgggattt tcgtcgattg cgaataggac cactggcgca
      721 aacaacagca gcgctatcgc gcaaaatctg acagcaagct tcatgctacc cttacctcct
      781 tcaagtctcg gtttgaaaac atcccggcgc tgctaggtcg gctgtcgtcg tggcgctgtt
      841 ttaatgcgga tttgcgcagc gcgcaagcgg ccggcgtatg cctccaactt ttgcgcttgt
      901 gcgcgcggat gaaacttgtc cttccgattt aaattcatga cgagaatttt cctcgcgcga
      961 ttcatttcgc aatgtttatt gatttgcttg acagtggagc gggttcatca atagttgtcg
     1021 ctagagagat tgcgtttacg aatcatagaa gaaggcttca gtgagctgcg ctaattttca
     1081 gcaagaacgg tttgtaaagg cctgtcaatc aggatcttgg aggagaagag atggcagagg
     1141 agatactcgt tgtgcacggt gggaaattga tcgatggcac cggtcggccg cccatcgaca
     1201 attcgaccat ccttattcgg ggaagaaagt tcaccgcggt gggcacagac ggcgaagtcg
     1261 ctgttcccgc gggcgcccag gtgatcgatg tcaccggcaa aaccgttctg ccggggttca
     1321 tcgatggtca cggccacttg gaggatttcc acggagagat ctacctccat ctcggcatca
     1381 ccacctgcgc caatatcgag ctttaccagg atgggccgtg gactctggct caaaagcagg
     1441 gcaccgatct gggtaaaatt cgcgggccgc gaatctggat gtcaggccgg gcaattggcg
     1501 gcgttagcac ggggcacgat gccttcggtt cgagaacctt tcgcgacaac atcatcgtca
     1561 caaccgccga tgaagtgcgt cgcgcggtgc agcgcaaaaa agaactcggg tgcgacattc
     1621 tcaaggtcaa tgaatttctt tctctggatc tggtgaaagt cgctgttgag gaggctcatc
     1681 ggttggacat gccggttgcc gcccatagtt gggacgtggt cggctattgc gatgcagggg
     1741 ttgacgccat cgagcacatc tggtccgtag gttacagctc catcccttat gcgccggccc
     1801 ggcgcaagct ggccgaggat cggctcggcg gcgtcatcga tcaggagctt gccggcgcct
     1861 attaccaaac agagaatttt gacgaggtga tcggcgcgat ggtgcagcat caggtggcgt
     1921 ggactccgac cattgccaaa tggttgcgac cgctctcgcc gagcgcgaat cgtttcaggg
     1981 aaagggaaaa ccaaatcttg aacgaccctg aagccgatct tcccgccgcc gttcgcgcgg
     2041 ttacggaaaa ttcttatgac aaattactca agcgctacac gcccgagcag cgccagcgcg
     2101 cgaggatcgg ctatgacaaa gccaatgagt tcatccgccg tttcgttcaa gccggcggta
     2161 tcctgaaaga ggggtcggac ccgccacgcg ggatggcggc cttgttgatg catcaggcgc
     2221 tggtgatgga tgtcgaggcg ggtgtctcgc cgatgacggc gatccaagct gccactctca
     2281 acgtcgctcg aactttcggc agggacaagg attatggcag cgtcgagccc ggcaaggtcg
     2341 ccgacctgtc gattgtcgac ggggatccgc tcaaagacat ctggatgacg cagaacgtca
     2401 agatggtcgt catggatgga aaggtgatcg atacaggatt taaaagatac aagaatccga
     2461 ttccgtcatt ctattcattt cagagcttgc cgttggatct cgagatagcg ccgctctttc
     2521 tcaccgaggg ctctggtccg acggtgttga gggtgcgtgg tgcgggcgga atgtggccat
     2581 tccaccaagt catgctcaac ggcgagccgt tgccaacgag cttcgtgtcc aaagacgaac
     2641 tcaaggcgat cattccgccg gaggcgatcg caaaggcggg gacatacatc gtcaccctca
     2701 agtgcgaagg agagcctttt ccggagtccc atcgagccca cctcgtggtt ggcttcaagc
     2761 cgtagatgtt ctcgccgaag gggaaactcg catcttcgga tcataatcat ccggtgattc
     2821 ataaaccccg gttatcgagg ttgcgaattg cttccacgga acgtaacgag tcggactgcg
     2881 ccacaggaac cgggaaggtc ttcaaccgtc acgagaatag tttttcgttt cttggcgtaa
     2941 gcgggaaaac taagctcctt tcttgaaacc gctgagccag ccgtgcaaca ctttgttcag
     3001 agcgtctaaa cttatggaac ttacgatcga gacgaattta agactcaatg acggtaatct
     3061 gattcctcag ctcggactgg gcgtgtggca gattggcccg gctaagacgt ccgacgcggt
     3121 gctcgcggcg ctggaagcga actaccggca tatcgatacc gcggcagcgt acggcaatga
     3181 agagagcgtg ggcgccgcca tcatgacgag cggcattccc cgggaacgca ttttcgtcac
     3241 gaccaagttg tggaacagcg atcacggtaa tccggagcgg gcgctcgaca ccagtctgcg
     3301 caagctcaaa atggattatg tggatttgta cttgattcac ttcccggtgc ggcaacggcg
     3361 gcaaagctgg cgcgctctgg aagggctacg ggcaaaggga aagacccgtt cgatcggtgt
     3421 cagcaatttc accattgcgc atctaaccga gctacttgct gagagcgaaa ccgtcccggc
     3481 agtcaaccaa gtggagtttc atccttattt gtatcagcag gaattactgg ctttctgcca
     3541 ggccaaagga atcgtcatcg aggcctatag cccgttgact catggagaac gactgaagga
     3601 cccgaagctt gtcgcggtcg caagaaaata ttcatcatct aaacccaaat ccacggcgca
     3661 aatcctcatc cgttgggcct tgcagcacgg cttggtggta attcctaaat catccaatcg
     3721 ccggcgtatt gtcgaaaacg ccgatgtttt cgagtttgaa atatcagaag atgacatgcg
     3781 gctgctggat agcttcaatg aaaacctgcg aacctgctgg gatcccagcg atactccatg
     3841 agcggggagt ccgcaaaata ttcttcttac gagatcttta ccggtacctc gaaggctgtg
     3901 cgctaaaaag tggtttcagc gcctgcgctg cggctggcct atttgcatcg cctcgcgcgc
     3961 ggcgttgtta gcgtggcggg atggtccaga gataaacgga acgctcgcga ccgctgcttc
     4021 gaacgcgcac gactttttgc ggcttccagt cgcccatgtc atgccaatgc gagacgatgc
     4081 gggcccccgg cttgagctcg cgcaacagtt ttggccgcag ttttagattg agctcggggg
     4141 agaggaagag catgaccact gatgcgtcgc tcacatctgt ctcaaaaagg tcctgattga
     4201 gaaaacggat gttttcagta acgccggcgg cgcgggcgtt ttcctggctt tcagcgatgc
     4261 gctggggatc gatatcgaca cagatgccgc gggcgccgaa ctctcgaact gcggtgatga
     4321 cgatccgccc gtcgccgcat cctagatcgt agaccacctc acctgatttc acgtttgcca
     4381 gccgaaacat cacctgcatc acatccggtg atgtgggttc gtagcgcacg tccggttcgc
     4441 gtagagaagt cgttgcggag gtcggctgct cgttacagcc aatcccggac aaagaagccg
     4501 caagaattat cccgcacaga acggttcgga gacttcttgc ggtccggcgc gagaccgaga
     4561 ccgatacccg ctgatccatt gatttcccgt cagcgcgcat gcgctccttt cctatgctca
     4621 tgactgcaaa atcccaccga cggga
//
LOCUS       DASUTG010000124         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_19681, whole genome shotgun sequence.
ACCESSION   DASUTG010000124 DASUTG010000000
VERSION     DASUTG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19681"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="alaS"
                     /locus_tag="VF089_02745"
     CDS             <1..1749
                     /gene="alaS"
                     /locus_tag="VF089_02745"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6172905.1"
                     /translation="NEGRGYVLRRLLRRAARHGRLIGLREPFLYQVASTVGEVMGEAY
                     PELRAEAQRIREIIRGEEERFSETLEKGLLILEDAAAKLKQQNQKMLPGEVAFRLYDT
                     YGFPLDLTEDILRTENISVDQQGFDNLMETQRSRGREARAVALDVFDQVAITDAVTAT
                     RFVGYDHLEVESTVVALYGNGASKTEAVEGDVIDLITAVTPFYGESGGQVGDRGTITS
                     ARGDLIQVLDTQHPTPQLTVHRGKVKKGRIQVGDKVHLQVDHEKRQKTRLNHSATHIL
                     HAVMREQLGSHVRQAGSLVTPERLRFDFNHTGAISDEKLADIETTVNDRIRQDAAVSI
                     EETSFDEALRKGALAFFGDKYGDRVRVVKIGDFSTELCGGTHVHRSGQIGIFKLHAEA
                     GVAAGVRRVEAVTGEGALGLIRRYEDDLKQIGHMLRADQDGAVERVRKLLQQQKELER
                     EIERLRAQLGKNRIPEILARKENINGIDVLITCIDEVDPKQLRELADQLKEKMFSGFI
                     FLASQGDNGVNLVASTSSDLAKRIHAGNIIKEVAPIVGGGGGGRPDFAQAGGKNPART
                     DEALAEVKKIVSAKLS"
     gene            1749..2174
                     /locus_tag="VF089_02750"
     CDS             1749..2174
                     /locus_tag="VF089_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6172906.1"
                     /translation="MKLKTAKLTETTQGFCDIIDITAKVLAQVRDAEIKDGLVLLFVT
                     GSTAALTTIEHEPGLVQDLKELVEKLIPSDRRYHHDDRWGDDNGFSHLRASLFGPSLQ
                     IPIAHGRLVLGTWQQIILLDFDNRPRTREIVVQLIGGRD"
     gene            2195..3196
                     /locus_tag="VF089_02755"
     CDS             2195..3196
                     /locus_tag="VF089_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6172907.1"
                     /translation="MTSAFPKEPAHLELHDPRLFRDLLGQHDQHIKILQQALDVKIRV
                     RGSSLEIEGDPLQMELASQIVRQLYGLLEKGYPVYPSDVDYAIRILSGDSRAKLQDIF
                     LDTIYISAHKRTITPKSIAQKAYIDAIRRYDIVFGIGPAGTGKTYLAMAMAVAELMKN
                     NFVRIILTRPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMVDFDRARKLVERGTI
                     EVAPLAFMRGRTLNDSFVILDEAQNTTPEQMKMFLTRLGYGSKAVITGDATQIDLPAG
                     KASGLKEAFRILKGVHGIRFVNFTEKDVARHHLVQEIITAYERDENQIASGDLSDKR"
     gene            complement(3235..4377)
                     /locus_tag="VF089_02760"
     CDS             complement(3235..4377)
                     /locus_tag="VF089_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6172908.1"
                     /translation="MDNKLHNAGLSHLSRWIPIGAVVSLASLAFVLGPQPWVDSATLN
                     DPKAAPRTVSPRGELWPDEKLTIGLFRQASPAVVNITAIGVQRDLFTLNLYQIPQGTG
                     SGFVWDTKGNIITNFHVIQNAAAAQVTLADQSNWKARVVGVAADKDLAVLRIEAPADR
                     LRPIPVGTSKELQVGQSVFAIGNPFGLDQSLTTGVISALGREIDSVTRRPIQGVIQTD
                     AAINPGNSGGPLLDSAGRLIGVNTQIYSPTGASTGIGFAIPVDTVNRIVPELIRSGKI
                     IRPGLGVELAEEQIARKIGVSGVLIVEVMRGGPAAKAGIRPTRRESSGRVILGDVIIA
                     IDGKKVESPNDLFLILENHQVGDTVTLALLRDGKTIQTKVTLEAVS"
     gene            4520..4966
                     /locus_tag="VF089_02765"
     CDS             4520..4966
                     /locus_tag="VF089_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6172909.1"
                     /translation="MESTRWEPYDGLNRLQARIHDLFDESLNRARSYPNAAGGTWLPP
                     VDILEGKEAYLIRAELPGMKKENFNLEFNDGTLTLSGERKFDEPANGVEYHRVERVSG
                     KFSRSFHLPQTVKPDAISANYRDGILEVQVPKADEAKPRQIAINVS"
BASE COUNT         1275 a         1349 c         1402 g         1088 t
ORIGIN      
        1 aatgaagggc gtggctacgt cctgcgacgg ttgctcagaa gggcggcgcg tcacggtcgc
       61 ctgatcggat taagggaacc gtttctatac caagtcgcga gcaccgtcgg agaagtaatg
      121 ggcgaggcct atcctgagct ccgagctgaa gcacagcgga tccgtgagat catccgcggc
      181 gaggaagagc gctttagcga aaccttggaa aagggactgt tgatcctgga agacgcagcc
      241 gcaaaactca aacagcaaaa ccagaagatg ttgccaggcg aagttgcgtt tcgcctgtat
      301 gacacttacg gttttccgtt ggacctgacc gaagacattc ttcgcaccga gaatatttcc
      361 gtcgatcagc agggtttcga caatctcatg gagacacaac gatcccgggg tcgtgaagcg
      421 cgcgcggttg cgctggacgt ttttgaccag gtcgcgatca ccgatgcggt gacagcgact
      481 cggttcgtcg gctatgacca tctcgaggta gagtcgacgg tcgtcgcgct atatggcaac
      541 ggagccagta aaacagaagc ggtggaaggc gatgtaattg atctaatcac cgctgtcacg
      601 cctttttacg gagagtcggg cggtcaggtt ggtgatcggg gtaccattac aagtgcgcga
      661 ggcgatctca tccaagtact ggacacccaa catccgacgc cgcaactgac agtccatcgc
      721 ggtaaagtaa agaaaggacg gatccaagtc ggtgacaaag tgcatctgca agtcgatcac
      781 gaaaagcgcc agaagaccag attaaaccac tcggcgacgc atatccttca tgcggtgatg
      841 cgagagcaac taggcagcca tgtgcggcag gccggttctc tggttacgcc ggaacggctg
      901 cgtttcgatt ttaatcacac cggtgcaata tccgatgaga aacttgccga tatcgaaacc
      961 actgtcaacg accgaatccg gcaggatgcg gcggtctcga tcgaggagac aagttttgat
     1021 gaggctttgc gtaaaggcgc cttagcgttt tttggcgata agtacggcga tcgagtgcgc
     1081 gtggtaaaga tcggcgattt ttcgacggag ttatgcggcg gcactcacgt tcatcgctcc
     1141 ggccagatcg gcatattcaa gctccatgcc gaggcaggtg tggctgcggg ggtgcgacgg
     1201 gtcgaagcag tcaccggtga aggagcgctc ggcttgatcc gacgctacga ggacgattta
     1261 aagcagatcg gccacatgct gcgcgccgac caagacggcg ctgttgaacg cgtcagaaag
     1321 ctgttgcagc agcagaagga actcgaaagg gagatcgagc ggctgcgggc tcaactcggt
     1381 aagaaccgga ttccggaaat cttggccaga aaagaaaaca tcaacggtat cgacgtctta
     1441 atcacctgca ttgacgaagt cgatcccaag caattgcgcg agcttgccga tcagctgaaa
     1501 gaaaaaatgt tcagcggctt tatatttctc gccagtcaag gagacaacgg agtaaatctt
     1561 gtggcgagca cgagcagcga tcttgccaag cggatccatg ccggaaacat aatcaaagaa
     1621 gtcgcgccca ttgtcggcgg tggcggcggc ggccggcctg atttcgctca agctgggggc
     1681 aagaacccgg cgcgaacaga cgaagccttg gctgaggtta agaaaatcgt gagcgcaaag
     1741 ctgtcttgat gaaactgaaa accgcaaagc tcaccgagac tacccaaggt ttttgcgaca
     1801 tcatcgatat taccgccaaa gtgctagcgc aagtccgaga cgccgagatc aaggacggcc
     1861 tggttcttct tttcgtcacg ggttcgactg ctgctctcac tacgatcgaa cacgagccgg
     1921 gacttgttca agacctcaaa gagctcgttg aaaaattgat tccgtccgac aggcgctacc
     1981 atcatgacga tcgatggggt gacgataacg gattttctca tctacgcgca tcactatttg
     2041 gaccatcact gcagattccc atagcacacg gccggctagt tctcggcacg tggcaacaga
     2101 tcatccttct tgatttcgac aaccggccac gaacaaggga gatagtcgtc cagctgatag
     2161 gaggtaggga ttgaaagacg agaagaatag attcatgacg agtgcgtttc caaaggaacc
     2221 cgcgcacttg gagcttcacg atccacgact gttccgcgat cttttaggcc aacatgatca
     2281 gcatattaag attctccagc aggccctaga tgtgaagatt cgcgtgcgtg gttcgtcgct
     2341 cgaaatagag ggcgaccctc tgcagatgga gcttgccagc caaatcgtcc gtcagctgta
     2401 cgggctattg gaaaagggat atccggtgta tcccagcgat gtcgactatg ctattcgcat
     2461 tctcagcggc gacagcaggg ctaaactaca ggatatcttc ctagacacga tctatatctc
     2521 agcgcacaag cggacgatca ccccaaaaag catcgcgcaa aaggcttaca tcgatgcgat
     2581 tcggcgttac gacatcgtat tcgggatcgg ccccgcaggt actggcaaaa cttatctggc
     2641 aatggccatg gcggttgccg agttgatgaa aaacaacttc gttcgcatta tccttactcg
     2701 cccggcggta gaggccggag agaagctagg tttcctgccc ggcgatttag cagaaaaagt
     2761 caatccttat ctgcgaccgc tgtatgacgc gctgcatgac atggtcgact tcgatcgggc
     2821 gcgaaaattg gtcgagcggg gcaccatcga ggttgctcca ctagccttca tgcgcgggcg
     2881 gacattgaac gattccttcg taatcctcga tgaagcgcag aacacaaccc ccgaacagat
     2941 gaagatgttt ctaacccgcc tgggctatgg ctcgaaggcg gtcatcaccg gcgatgccac
     3001 gcagatcgat ttacctgcag ggaaggcttc gggactgaag gaggcctttc gtattctaaa
     3061 gggggttcac ggcatccgat tcgtcaactt cactgaaaaa gacgttgccc gtcatcattt
     3121 ggtgcaagag atcatcaccg cctacgaacg cgacgagaat cagattgcct ctggagatct
     3181 ctcggacaaa agataaatca aactttcgtg attttcgcga acaacggggc tcgatcaaga
     3241 cactgcttcg agtgttactt tcgtctgaat ggtttttccg tcgcgcagca gcgctagcgt
     3301 cacggtatcg ccgacctggt gattctccag gataagaaaa aggtcattgg gtgactccac
     3361 ctttttacca tcgatagcaa taatcacatc accgaggatg acgcgtcctg agctctcacg
     3421 ccgcgtcggc cgaattcctg ctttggccgc cggaccgcca cgcattacct cgacgattaa
     3481 aactccgctg acacctatct tcctggcgat ctgttcctcc gctaattcga ctcccaggcc
     3541 aggtctaatt atctttccac tgcgaatcaa ctccggcaca atccgattaa ccgtgtcgac
     3601 tggaatggca aagccgatac ccgtcgacgc tcccgttgga ctatagatct gggtattgac
     3661 accgatcaaa cgtccggcgc tgtcgagcaa cggaccgcct gagtttcccg gattgatcgc
     3721 cgcgtcggtt tgaatgaccc cttgtatggg acggcgggta accgaatcga tttcgcggcc
     3781 aagcgcactg atcacgcccg tggtcaacga ttggtccagc ccgaaagggt tgccgatggc
     3841 aaacacgctc tggccgactt gtaattcctt ggatgttccc actggaatcg gacgaagccg
     3901 atcagccggc gcctcgattc gtagcaccgc tagatcctta tcagcagcaa cgccgactac
     3961 ccgtgccttc caattggatt gatccgctaa cgtcacctgg gctgcagccg cattctgaat
     4021 cacatgaaaa ttggtgatga tgttgccttt ggtatcccaa acaaatccgg agccggttcc
     4081 ttgcggtatc tggtaaaggt tcaatgtaaa caaatcgcgt tgtactccga tggcggtgat
     4141 gttgacgacc gccggggatg cttgtctaaa tagcccaatc gtcaactttt cgtcaggcca
     4201 aagttcgccc cgtggagaaa ccgtacgcgg ggcggccttc ggatcattta acgtggcgct
     4261 atcaacccag ggttgcggac ccaatacaaa cgccagtgat gcaagcgaaa ccacagcccc
     4321 gatgggtatc caacgagaga ggtgagagag cccggcgtta tgaagcttat tatccatacc
     4381 tgcagtcctt atttgtggag cttcgatcaa ccgctaaacg tagggaccgg tgagattatt
     4441 gtcaagccga ttgactcgtc aacggcccgt gcttaggttt caatcacaaa atctcacgcc
     4501 gcctggaagg agggcaatca tggaatcgac aagatgggaa ccttacgatg ggctgaacag
     4561 actgcaagct cggattcacg atctattcga tgaaagcctg aatcgagctc ggtcgtaccc
     4621 gaacgcagct ggcggaacgt ggctaccgcc ggtggatatt ctggagggca aggaggctta
     4681 cctgattcgg gccgaacttc cgggaatgaa gaaagaaaac ttcaacctgg aattcaatga
     4741 tgggactctg acccttagtg gggagagaaa atttgacgag cccgcaaatg gcgtagagta
     4801 ccatcgcgta gaaagagttt ccggaaagtt ttcgcgatcg ttccacctgc cgcaaacagt
     4861 taagcccgat gcgattagcg ccaattatcg cgatggcata ctcgaagtgc aggttcctaa
     4921 agcggatgag gcaaaacccc gccagatagc gatcaatgta agctgagaca tcgcagagac
     4981 ccggggaaga taaacctccc ccgggtcttc tctttcactg cctggggaag tagtacctgc
     5041 tcacctgcgc tccgcagttt tgttgaacca gttaacccct tttccggacc gtacttgtcg
     5101 cgtacaaaca aaaa
//
LOCUS       DASUTG010000125         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1946649, whole genome shotgun sequence.
ACCESSION   DASUTG010000125 DASUTG010000000
VERSION     DASUTG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946649"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VF089_02770"
     CDS             complement(<1..1784)
                     /locus_tag="VF089_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6172910.1"
                     /translation="MTYPIINITIEYEHDVVLARQRARELAALLGFDNQDQTRLATAV
                     SELARNAFVYAGGGKLEFEIEGRTVPQLFVVRVSDRGPGIENLKEILEGRYASRTGMG
                     IGLIGARRLVDQCEIHSDSKQGTKITLRKIMPRHAPLVATTSLRSLVDQLAQRRAPGP
                     LEEIQQQNRELVRALAELRERQEELARINRELEDTNRGVVALYAELDEKASHLRRADE
                     MKSRFLSNMSHEFRTPLNSILALSGLLLDRADGELTSEQEKQIGFIRKGAETLLELVN
                     DLLDLAKIEAGKIEVQPVDFEVANLFSALRGMLRPLLLTQTVSLVFDEPAEIPCLYTD
                     EGKLSQILRNFISNALKFTEQGLISVTATFNKADQTVTFAVSDTGMGIPAEDQAKIFE
                     EFVQLENPAQRKIKGTGLGLPLCRKLAALLNGKIDLVSQIGIGSTFSVTIPLYYSQVP
                     ESQAGGETKPVPSWEIDETRIPVLVLEDAPETRLLYEKFLRRSAFQLIPANNLAEARR
                     ILRQVRPGAIVLDILLRGEDSWTWLAELKTESSTREIPVLVITSVDDERKGLSLGADA
                     YCLKPIGRDTLISQLHALTGLNPTISQA"
     gene            complement(1890..2921)
                     /locus_tag="VF089_02775"
     CDS             complement(1890..2921)
                     /locus_tag="VF089_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HEX6172911.1"
                     /translation="MIPLWERQKVLEVSEPSHVAQVRRFTSELAEDGGFNESQVGGVA
                     LVTTELATNLLKHAGRGEIVARPIVDRGIAGVELLALDKGLGIVNLAESLRDGYSTSG
                     TAGTGLGAIARLAAEFDIQTQSGKGTAVMARLWAGKLAEESEDRIAIGAICKPMAGEK
                     RCGDGWGCEFLADRCILVLVDGLGHGLGAAAAAETAVANVRQHCAKAPAAIVEYAHDA
                     LRATRGAALAVAEINWAQQSLRFCGIGNISATIITGDNLRHLVSYNGTAGHEARKIVE
                     FTYPWSADALLIMHSDGLMTRWNLQAYSGLLQRHPSLIAGVLYRDFVRGRDDVTVLTA
                     RSVVARSAE"
     gene            complement(2918..3328)
                     /locus_tag="VF089_02780"
     CDS             complement(2918..3328)
                     /locus_tag="VF089_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX6172912.1"
                     /translation="MAVLKDETAPLRSSDDIVRVRRIVRDWAVSLGFSLVDQTKVVTA
                     ASELARNAIDHGGGGDFRIESLNNDARRGLRLTFSDEGPGIVDMEQAMKDGFTTGGGL
                     GLGLGGAKRLVNEFLIDSKPGRGTTVVITRWKNL"
     gene            complement(3309..3698)
                     /locus_tag="VF089_02785"
     CDS             complement(3309..3698)
                     /locus_tag="VF089_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6172913.1"
                     /translation="MERIPILKMGRFLLVTIQVDMHDRLAMTLQDDLTDRIVKDHAQG
                     VLLDISSLEIVDSFIGRMIGHIAGISRVLDAKTVVVGMRPAVAITLVELGMTLDGVST
                     ALNVDAGMAMLSASLEEREQLNGGSQR"
     gene            complement(3706..4566)
                     /locus_tag="VF089_02790"
     CDS             complement(3706..4566)
                     /locus_tag="VF089_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6172914.1"
                     /translation="MIEEQSKVAQLLRKHERDVLQHWLSYQISATTMRADLIKESDLR
                     EQSRRFLQLFTDVLQQNGEGENAGSWKSVKDMLTEVSRSRVAQGFSPSETATFVFSLK
                     QPIFDMLRREIRDIEELCREIWVTTALLDKLGLYTTEAYLQSRDSIISRQQEELMELS
                     TPVVQLWDNVLALPLIGTLDSERTQVVMENLLEKVVETGASIAIIDITGVPTVDTLVA
                     QHLLKTVAAARLMGADCIISGIRPQIAQTIVHLGINLADIITKATLADAFAIALQRTG
                     AKVTRLSGKQ"
     gene            complement(4975..>5428)
                     /locus_tag="VF089_02795"
     CDS             complement(4975..>5428)
                     /locus_tag="VF089_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6172915.1"
                     /translation="LLVQDFLHRHPMAARKGISSISKQAMRRLLDYSWPGNIRELQNV
                     LERAIVLNTGRTIEHVDLPEVSFEAHPSKGGNAFDAPLNQWLQEKEKEYLAQKLDDFG
                     GNVGLTAKSCRIGVRTLSRKMRAYGLDKKLFKQKTDVAKTIPPKTGQA"
BASE COUNT         1176 a         1585 c         1448 g         1219 t
ORIGIN      
        1 tctgcttgcg agattgtagg atttaggccg gttagagcgt gaagctgact aatcagagtg
       61 tcgcgtccga tcggctttag gcagtaagca tcggcaccca gggataagcc cttgcgttcg
      121 tcgtcgacac tcgtgatgac cagtactggg atctcccggg tagagctctc cgtcttgagc
      181 tcggccaacc aggtccacga gtcttcgccg cgcagaagaa tatccaaaac aatcgcaccc
      241 ggccgcacct gccgtaggat tcggcgggcc tcggccaagt tgttcgccgg gatcagctgg
      301 aacgcagatc gacgaagaaa tttttcatag aggaggcgag tctccggagc gtcctcaagc
      361 accagaaccg gaattcgtgt ttcatcgatt tcccacgaag gcaccggctt cgtttcgcca
      421 cctgcctggc tctccggcac ctgagaatag tagagtggaa ttgtcaccga aaacgttgaa
      481 ccgattccta tctgactcac caggtcgatt tttccgttca gcaatgcagc cagcttgcgg
      541 cacaggggga ggccgagacc cgtgcctttg atttttcttt gtgccggatt ttcgagttgg
      601 acaaattcct cgaaaatctt cgcctgatcc tcagcgggaa tacccattcc ggtatccgag
      661 actgcgaagg tcactgtttg atccgcttta ttgaacgttg cggtgactga gatcagaccc
      721 tgttccgtga atttcaacgc gttcgaaata aaattgcgta agatttgcga cagcttgcct
      781 tcatcagtgt ataaacaagg aatctccgcg ggttcgtcaa aaaccaggct cacggtctgg
      841 gtcagcagga gcggtcgcaa catgccgcgc agcgcactaa aaagattggc cacttcgaag
      901 tctaccggct gaacctcgat cttgccggcc tcgatcttag ccaaatctaa cagatcgttg
      961 accaactcca gcagagtctc cgcgcccttt cggatgaagc cgatttgttt ttcttgctcg
     1021 ctcgttaatt ccccgtcggc gcggtccagc aacaatccgg ataatgccaa gatcgaattt
     1081 agcggcgtgc gaaattcgtg gctcatgttg gaaagaaacc gtgacttcat ctcgtcggca
     1141 cgacgcaaat gactggcctt ctcatccaac tcggcataaa gcgccaccac gccgcggttg
     1201 gtgtcctcaa gctcccgatt gatccgcgcc aactcttctt gccgctcgcg caactccgct
     1261 aaagcccgca ccaactcccg attttgctgt tggatctcct ccagcggacc cggcgctcgc
     1321 cgctgcgcca gttgatccac caaactgcgc agcgaggtgg ttgccaccag cggcgcatga
     1381 cgcggcataa ttttccgaag agtaatcttg gtgccctgtt tggaatcgct gtggatctcg
     1441 cattgatcga ccagtcgacg ggcgccgatc agcccaatcc ccattcctgt gcgcgacgca
     1501 taacgccctt ccaaaatctc tttgagattc tcgataccgg ggcctctgtc gctgacccgg
     1561 acaacgaaaa gctgcggcac ggtgcggccc tcaatctcga actccagctt cccgccgccg
     1621 gcataaacaa aagcgttgcg cgccaactcg gaaaccgcgg tggcaagccg cgtttgatct
     1681 tgattatcga agccaagcaa tgcggcaagc tcacgcgccc gctggcgcgc cagcacaaca
     1741 tcgtgctcgt attcgatggt gatgttaatg atcgggtagg tcataggcgg ttttggtttg
     1801 tcgttctggt actggttctc aaatcagctc ctcgaaaaga ttgagcgaac ttaaccgacg
     1861 gatcgcgatt aagtggatcg ccggggtttt tactcagcac tccgcgccac aacgcttctg
     1921 gcggtaagga ccgtcacgtc atcgcgaccg cggacgaaat cgcggtagag gacaccggcg
     1981 ataaggcttg gatgacgttg aaggagccca gagtaggcct gaagattcca ccgcgtcatc
     2041 agtccgtcag agtgcatgat caacagcgcg tctgcgctcc atggataagt gaactcgaca
     2101 atcttgcgcg cttcatgacc ggcggtgccg ttgtaagaaa ccaggtggcg caggttgtct
     2161 ccagtgatga tagtggcgga aatgtttcct atgccgcaaa aacggagcga ctgctgagcc
     2221 caattgatct ccgccactgc gagagccgcg ccgcgcgtgg cgcggagcgc atcgtgagcg
     2281 tattcaacta ttgccgccgg agctttggcg cagtgctgcc gcacgttggc gacggccgtt
     2341 tcggccgccg ccgcagcacc taatccgtgc cccagaccat cgaccagtac caaaatgcat
     2401 cgatccgcga gaaactcgca accccatcca tcaccgcaac gcttctcgcc cgccatcggc
     2461 ttacagatcg ccccaatagc aatgcgatcc tcagattctt ccgctagctt accagcccag
     2521 agccgggcca tcacggcggt gcccttcccg gactgcgttt ggatgtcgaa ctcggcggcc
     2581 agccgggcga tagcgccaag accggtcccc gcagtcccac tagtcgaata gccgtcgcgc
     2641 agagattcgg caaggttcac gattcccaac cccttgtcga gagctaataa ctcgacaccg
     2701 gcgatgcccc ggtccacgat gggtcgcgcg acgatctcac cgcggccagc gtgcttaagc
     2761 aggttggttg ccaattcggt ggtcacgagc gctacgccgc cgacttgaga ttcattaaat
     2821 cccccgtctt cggctagttc gctggtaaaa cgccgcacct gggcgacgtg actgggctcg
     2881 cttacttcaa gaaccttttg cctttcccaa agagggatca tagattcttc cagcgagtaa
     2941 tcaccacagt agtgcctcgc ccgggttttg aatcgatcag aaattcgtta accaaccgct
     3001 ttgcaccccc tagtccgaga cccagccctc cgccggtggt aaacccgtcc ttcattgctt
     3061 gctccatatc aacgatacct ggtccctcat cggaaaacgt caaccgcagc ccgcgccggg
     3121 catcattatt gagcgattcg atgcggaagt caccaccgcc gccgtgatcg atggcattac
     3181 gcgccagctc gctggctgcc gtgacgacct tggtctgatc cactaagcta aaccccagcg
     3241 atactgccca gtcgcggaca atccggcgca ccctgacaat gtcatccgaa ctccgcagtg
     3301 gagcagtctc atctttgaga accgccatta agttgttccc tctcttccag cgaagcgctc
     3361 agcatcgcca taccggcatc gacatttagc gccgtgctga ctccgtcgag cgtcatgccc
     3421 agctctacca gcgtaatggc aacggcaggt ctcatgccca ccaccacggt tttagcatcg
     3481 agcacgcgcg agatgccggc aatgtggccg atcatgcgac caataaacga gtcgacgatt
     3541 tccagcgagg aaatatcgag caacacgcct tgtgcgtggt ctttgacgat acgatcggta
     3601 aggtcatctt gaagcgtcat cgccaagcga tcgtgcatgt ccacctgaat cgtcactagt
     3661 agaaagcggc ccattttcag gattggaatc cgttccataa ttacctcact gtttgccgga
     3721 caggcgcgtc accttggctc ccgttcgctg cagcgctatc gcgaatgcat cggccaaggt
     3781 cgctttagtg atgatatctg cgaggttgat tcccaggtga acaatggtct gggcgatctg
     3841 cggacgaatg ccgctgatga tgcaatcggc tcccatgagc ctggcggcgg cgaccgtctt
     3901 cagcaaatgt tgcgcaacca gtgtatctac ggtcggaacg ccagtgatat cgatgattgc
     3961 tatcgaggcg ccggtttcga cgactttctc gagcaggttc tccatcacca cttgagtgcg
     4021 ctcactgtcg agcgtaccga tcaatggcag cgcgaggaca ttgtcccaga gctggaccac
     4081 cggagtggat agctccatca gttcttcctg ctggcgcgag atgatcgaat cgcggctctg
     4141 aaggtacgct tccgtggtgt agagtccgag tttgtcgagc agagccgtcg ttacccaaat
     4201 ctcgcggcac agttcttcaa tgtcgcgtat ctcgcgacgc aacatgtcaa agatcggctg
     4261 cttgagggaa aaaacgaacg tggcggtttc agacgggctg aaaccctggg caacccggct
     4321 gcgcgatact tcggtcagca tgtctttgac cgatttccag gagccggcat tttcgccctc
     4381 cccgttctgt tgcaaaacgt cggtaaagag ttgcagaaag cgccgcgatt gctcgcgcaa
     4441 atcgctctcc ttgatcaaat cggctcgcat ggttgtcgcg gagatctggt acgacaacca
     4501 gtgctgtaaa acatctcgtt catgctttct aagaagctgg gcgactttcg attgctcttc
     4561 tatcattgtt tttcttcttt cggtttgatg ttggttacgt ttagtacgca caggatcaaa
     4621 cacctcgcaa taaactcctt aggttagtca acgactaggc cctcaaccgc cgctcagcac
     4681 cggatcggcg cacttcgctg gttgcttgtc gccagctaac cagcgatgag aatgttttca
     4741 tgatggtctc tgtgttggag tgcagcgagt gataatcaat ccggggtgta acagaagaac
     4801 cttggcccta tgtctagcaa attacggtgg agttatacaa tcccaaaatt gtgcaaagtt
     4861 gagcctagcg aggcgcgcac ctccggcact aattcaaagg tgcatgaaac aagtctcgta
     4921 ggaacagtag aagaagaaag acgggaacgg tgagctaaac tgtagagcga tagatcaggc
     4981 ttggcctgtc ttgggcggga tagtttttgc aacgtcggtt ttttgcttga acagcttctt
     5041 atccagaccg taggcgcgca tcttacggga aagcgtgcgc acgccgatcc ggcagctctt
     5101 tgcggtgagt ccgacgttgc cgccgaaatc atcgagcttt tgcgccagat actccttttc
     5161 tttctcctgc agccattggt tcaggggggc gtcgaaggca ttgccgccct tgcttggatg
     5221 cgcctcaaag ctgacctcgg gcagatcgac atgctcaatg gttctgccgg tgttaagaac
     5281 aatggcacgc tccaggacat tttgcagttc gcgaatgttg ccgggccagg agtagtccaa
     5341 cagccggcgc atcgcttgct tggaaatcga gctgatcccc ttgcgcgcgg ccatcggatg
     5401 gcgatggaga aaatcctgaa ccaacagc
//
LOCUS       DASUTG010000126         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_168403, whole genome shotgun sequence.
ACCESSION   DASUTG010000126 DASUTG010000000
VERSION     DASUTG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168403"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VF089_02800"
     CDS             complement(<1..448)
                     /locus_tag="VF089_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6172916.1"
                     /translation="MQDQTNRDAVHILYAEDDPPSGRLVRSIAESEGYTIKVVATGKE
                     FLSAIFEFKPNLLLIDLHLPDALGLDLLSQARARLPAAPAIVVTASASVDDVIGALKA
                     GAIDYLRKPVDIQRMIVSLANAAKIMRQQQDITQLRSELKETYRLEH"
     gene            1138..>3351
                     /locus_tag="VF089_02805"
     CDS             1138..>3351
                     /locus_tag="VF089_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6172917.1"
                     /translation="MSDRELGEVLIDSSPDALIAVAPDGTILFWNAGAEAVYGYTKAE
                     AIGNRLYDLIVPADLVQESKKIMRDAIEAGLTIHETVRRRKDGSVISVDITAKAVRDQ
                     HNRLRFLAVNQKDVTQLKVLNHGRALEAKYRGLLETVPDAIVMVNNMGRIILVNGQAE
                     ALFGYKREELIGKPVELLLPERYQTGHSSHRQNYFAGPKTRTMGADLELYALRADGTE
                     FPVEISLSPLDTDEGTFAMSAIRDITERKRAQELLRRSDERFRLLVTGVIDYGIVMLD
                     PQGNVVSWNIGAQRISGYKAEDIIGRHFSIFYPSDEVTSEKLARELKLAASEGRFESE
                     GWHVRQNGSRFWGHVVITALRDNTGELTGFSKVVRDLTDNKIAEELLQEQNRRVQEAS
                     RLKSQFLANMSHELRTPLNGIIGFAELMYDGRVGPVTVEHREYLSDILSSANHLLQLI
                     NDILDLSKIEAGKMEFVPEPTDPTIVVEEMRGIVRTLAASKRVTVKTEVDASLSEIQA
                     DPRSLKQILYNYLSNALKFAPDGGQVTVRLKSEDTDHYRIEVEDNGIGIKPEDVDRLF
                     IEFQQLDASAAKKYPGTGLGLALTKKIVEAQGGKVGVDSSPGKGSIFYAKLPRAFPPP
                     VVYVQKMANANPQLAQILVVEDDSNDRACIAGILQAAGYVVEAVATGAEALVRCRDQR
                     FDAITLDLMLPDMTGRSVLAKLRERGVNQHTPVIVVTLLPNKGIVAGFQVAAILRK"
BASE COUNT          839 a          828 c          894 g          790 t
ORIGIN      
        1 tatgctccag tctgtatgtt tccttcagct ctgaacgaag ctgcgtaata tcttgttgct
       61 ggcgcataat cttcgccgca ttcgccagtg atactatcat gcgctggatg tcgaccggct
      121 ttctgagata gtcgatggcg ccggctttaa gcgcgccgat aacgtcgtcg accgaagcgg
      181 aagccgttac aactatcgcg ggagcggctg gaagacgcgc gcgcgcctgg gatagcagat
      241 cgagtcccaa cgcgtccgga agatgcaaat cgatgagtaa gagatttggt ttgaactcga
      301 atatcgcgct tagaaattcc tttcccgtag cgacgacttt gattgtgtac ccttcggatt
      361 cagcaattga acgtactaat cgtccggagg gaggatcgtc ttccgcatac aggatgtgca
      421 cagcatcccg gttggtttgg tcttgcatcg gcaacagatt aatctgttgt ccgcaattcg
      481 gtcaatataa aatcattaca gactcatcac agtctcaccc agaaataaag ctttcgctgt
      541 taaaccgtac actgaagagc ttcatcgata aagccagtcg gagtacattc tacgtgcggc
      601 gagcaaagcc ggtcgaatgt tctgatactt gctggtcgat ctttaattca gcggccggga
      661 ggcgaatggg tttccggtct ccggcgtaaa tttttcgctg tcgccattct gcatacttgt
      721 acataactgt tcattttcgt cagcattatt tcccgtcttt cgctgtttgg tcgaacaaaa
      781 ctctcaccag agcaacatct tcctccttgg catttggttt gcctattgcg atcgactagg
      841 aaattgcggg cgcggttagg ctacttaaat gaatgatgat gttcatgttc ctggtaagct
      901 catcatctgt tacttaccac ccccgagatt cggatccttc ctcacagatg tcttctttga
      961 ttatctacaa cgcacctgtc tcagcatcgg ctagacggcg cgtccacggc gcctattcaa
     1021 aggccgagag ctgaaacgat gcgcgtcaga tagatgactg attaaagact gttgaatgac
     1081 atgacaaagc ccaacgaacc aagcaaagat aggagccacg aaaactcttc agcctcgatg
     1141 tcggacagag aacttgggga ggttttgata gatagctccc cagacgcact gattgcggtt
     1201 gcgcctgatg gaacgattct attctggaac gccggtgccg aagcagttta cggctacaca
     1261 aaagctgaag ccattggtaa tcgcctgtac gatctgatcg tgcctgcgga ccttgttcaa
     1321 gaatccaaaa agataatgcg agatgccatc gaagccggcc taacgattca tgagacggtt
     1381 cgccgaagga aagatggttc ggtcatctct gttgatatca ctgccaaggc ggtccgagac
     1441 caacacaacc ggctccgatt tcttgctgtt aatcagaaag acgtcactca gcttaaagtt
     1501 ctaaatcatg gccgagcgct ggaagcgaag tatcgtggct tgctggaaac tgtaccggat
     1561 gccatcgtca tggttaacaa catggggcga atcattctgg tcaacggaca agccgaggcg
     1621 ctattcggtt acaaaaggga ggaactcata gggaagccgg tggagctcct tctgccggaa
     1681 cggtatcaaa ccggacactc gagccatcga caaaattatt ttgccgggcc taaaacgcga
     1741 acgatgggcg cagatctcga actttacgcc ctccgggcag acggcactga gtttccagtc
     1801 gaaattagcc tgagtcccct ggacaccgat gaaggtactt ttgccatgag tgcgatccgc
     1861 gacattaccg agagaaaaag ggcgcaggag ctgttgcgcc ggagcgacga acggtttcgt
     1921 ttgttggtga ccggtgtgat cgactacggc attgttatgc ttgaccccca aggcaatgta
     1981 gtcagctgga atatcggagc acagcggatc agcggctata aagccgagga cattattggc
     2041 cggcattttt ccattttcta tccttctgac gaagtcacta gcgagaagct tgcgagagag
     2101 ctgaagctag cagcgtccga aggacggttc gaaagtgaag gctggcatgt acgccagaat
     2161 ggctcacgtt tttggggcca tgtggtgatc acggcgttgc gagacaacac tggcgaatta
     2221 actggtttct ccaaagtcgt ccgggatcta accgacaaca aaatcgctga agagctgtta
     2281 caggagcaaa accgtcgggt tcaagaagct agccgactaa agagccagtt tctcgctaac
     2341 atgtcgcatg agttgcgcac gcctctcaat ggaatcattg gtttcgcgga gcttatgtac
     2401 gacggcaggg ttgggccggt cacagtcgag catagagagt atctcagcga tatcctgagc
     2461 agcgccaatc acttgttgca gctgatcaac gatatcctgg atctttctaa gatcgaagcc
     2521 ggcaagatgg aatttgttcc ggaaccgacc gatccgacaa tcgtggtcga agagatgcgc
     2581 gggatagttc ggacgcttgc agctagtaag cgggtcacgg taaagaccga agtggacgct
     2641 tctctttctg agattcaggc ggacccgaga agtctgaaac aaattctata taattatctg
     2701 tccaacgccc ttaagttcgc gcctgacggc ggccaggtga ccgtacgtct aaaatcggag
     2761 gacactgatc attatcgcat cgaggtagag gataacggca tcggcatcaa gccggaagac
     2821 gtcgaccgct tattcattga gttccagcaa ctcgatgcga gcgccgccaa gaaatacccc
     2881 ggaacgggct taggcctcgc actaacgaag aaaattgttg aagcgcaggg cggtaaggtt
     2941 ggcgtggata gctcaccggg aaagggcagt atcttctatg cgaaactgcc tcgagccttc
     3001 cctcccccag ttgtttacgt tcaaaaaatg gcaaatgcca atccccagct ggcgcaaatc
     3061 ctggttgtcg aggacgatag taacgatcga gcttgcattg caggaatctt gcaggcagca
     3121 ggttacgtag tcgaggcagt cgctaccggg gccgaagcat tggtacggtg tcgggatcaa
     3181 cgttttgacg caattaccct ggatcttatg cttcccgata tgactggacg atctgtgctt
     3241 gcaaagctgc gggagcgagg tgttaatcag cacacgcccg taatcgtcgt tacgcttctg
     3301 ccaaacaagg gaatagtcgc cggatttcaa gttgccgcga tcctcagaaa g
//
LOCUS       DASUTG010000127         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_20205, whole genome shotgun sequence.
ACCESSION   DASUTG010000127 DASUTG010000000
VERSION     DASUTG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20205"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1433
                     /locus_tag="VF089_02810"
     CDS             156..1433
                     /locus_tag="VF089_02810"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX6172918.1"
                     /translation="MSLQTVHLDFDSLTADQQVACLKEHHVSLRGKGALVRARVHDLP
                     VRQYISLLERGYRVGLEREGGSFTLTLDPDGSTPRMGLRGAHSVVSHDDGRVYANTTE
                     NRIAVIDGATRRLRKHIPTGNDPSHLELSADGNRLYVANSGSNDVTIIDTTTDQVLTT
                     VPTAKRPLLPCVASNGSAIYLPSGPERSVTVLGADGELRARVAVGIAPHDIAVSPDSL
                     WAYQPNSGSHTVTVIDGRNHSVVGEMKVGLGPGHIAFDPESRFGYVANTVSDDVTVID
                     TAIHEAVASISAGPGAHLPVISPDGRYGYVANFASDNLTVWDCKNWRVITTIPVGIYP
                     HFFAISTDGDWLVVSNTGESSVCIIDAREHEIRARLQVGPAPAHLAFSPDNECAFVGC
                     ESSDEVAVINLRKQTVVEMVKAGQLEPKVCCSD"
     gene            complement(1671..>3244)
                     /locus_tag="VF089_02815"
     CDS             complement(1671..>3244)
                     /locus_tag="VF089_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6172919.1"
                     /translation="RYLVWDELKNQAVVYDPGTGGYERDNTQPALFGPRVLRVKSGEE
                     IACKPVFEKLGEIASAYAPERSEKITWVPAKDIWRTALLLAHNRPVSMYMWNGLGQHT
                     NATQTSRAIASLYALLGDYDRQGGNVIFPGLKVNDVGGKEFLPKEMAAIRIGREKKPL
                     GPPAKPGNCTAYDIFTAILEQQPYPVKAFLNFGSNTVLSTANSRRAREAMMALELGVA
                     ADLFMTPTAELCDYVLPATSFLEMASITTSFEHRPSGKTHMQYRPAVVKPAGERRSDT
                     WIIFELAKRLGFGEKFWDGDVDAAYAYELEPTGVTLAHLKQSPGGISLPAQPAFQKYA
                     ARNKDGTQRGFATPSKKVELYCETFAAHGYPALPEYIEPALSPVSRPDIAAEYPLVLT
                     NAKFTTFIHSQQRALPSLRKISPQPSADIHPETAALYGITNKEWMIVESPRGAIKVKA
                     RVTTNIVPGVICCQHGWWQDCKELDLPGYNPYNRNGANPSTLVGTEIADPVSGSLPHR
                     SYLCRIRPAAKPKQQ"
BASE COUNT          661 a          896 c          956 g          731 t
ORIGIN      
        1 tttttgttcg atttaaccgg agcctacgac ctttctttta tacttttcgg cgcagcactg
       61 attacatcgg cgttcctcag cctgatgttg cggccgccgg tgaagacagt ggccacacga
      121 ccaaactgac gaagcggcga ataaaggagc gttccgtgtc gttgcaaacg gttcatcttg
      181 atttcgacag cttaactgca gatcagcaag tagcttgcct caaagagcac cacgtttcac
      241 tccgcggcaa aggcgcttta gtgcgggcgc gcgtccatga tctgcctgtg cggcagtata
      301 tctctttact ggagcgcggc tatcgggtcg gattggagcg agagggcggc agcttcacat
      361 tgactctcga tccagatgga tccacgccgc gtatgggact ccggggcgcg cactccgtgg
      421 tgagccatga cgatggccgt gtttatgcaa atacgactga aaatcggatt gccgtcatcg
      481 atggggcgac ccggcgttta agaaaacaca ttcccaccgg caacgatcca tcgcatctgg
      541 aattgtccgc agacgggaat cgactttatg tcgcgaacag cggctccaac gacgtgacga
      601 tcatcgacac caccaccgat caagtgctga cgacagttcc taccgcaaag cgtccactgc
      661 ttccctgcgt cgcttcgaac gggagcgcca tctatctgcc gagcggcccg gaacggtccg
      721 tcaccgtgct cggcgccgat ggcgagcttc gcgccagggt tgcggttggg atcgcgccac
      781 atgacattgc cgtttcgccc gacagcctct gggcttacca gcccaactcg ggctcacata
      841 cggtgacggt tatcgatggc cggaaccatt ccgttgtcgg cgagatgaaa gtcggtctcg
      901 gtcctgggca catcgccttc gatcccgaga gtcggttcgg ctacgtggcg aacacggtgt
      961 cggatgatgt gacggtcatc gatacggcaa ttcatgaggc cgtcgcatcg atatcagcgg
     1021 ggccaggcgc gcatcttccg gttattagtc ccgatggacg ttacgggtac gtcgccaatt
     1081 tcgcctccga taatctgaca gtgtgggatt gtaagaactg gcgtgtcatt accacgatcc
     1141 cggtggggat ctatccgcac ttctttgcaa ttagcaccga cggcgattgg cttgttgttt
     1201 cgaacaccgg cgaatccagc gtctgcatca tcgacgctcg cgagcacgaa atccgagccc
     1261 ggctgcaagt cggtccagcc ccggcgcacc tggccttcag cccggacaac gaatgcgctt
     1321 tcgtcggctg tgagagcagc gatgaagtgg cggtgattaa cttaagaaag caaacggtcg
     1381 ttgaaatggt taaggcagga cagctcgaac cgaaagtatg ctgcagtgat tgatctgtag
     1441 tccgtcacct atggttttcc cctgcgagct tcagctatgg cgcgtgccaa gagtttgtcg
     1501 gaagtcgcat cctggtgctc gtccctactt gctggctgtg ccgaagtaga ggaatcaaaa
     1561 agaacaaaga tgacggggaa gatgttcctc tttagtgttc gggatgatac tcctcgaaag
     1621 aacctcctac ccggtggcgc tgctgcttct tgttagcgaa agcaaaatat ctactgctgt
     1681 tttggcttag cggctgggcg gatgcggcag agataagaac ggtgcggcag cgagccgctg
     1741 accgggtcgg cgatctccgt gccgacgaga gttgacggat tggcgccgtt tctgttgtag
     1801 ggattgtagc cgggcaggtc gagctctttg cagtcctgcc accaaccgtg ctggcagcag
     1861 atcacgccgg ggacgatgtt cgtggtaacc cgtgctttaa ctttgatcgc gccacgcggg
     1921 ctttcgacaa tcatccactc tttgttggta attccataaa gggcggcggt ttcgggatga
     1981 atatcggcgc tgggctgcgg cgaaatttta cgcaaactcg gcagcgcgcg ttgctggctg
     2041 tgtatgaacg ttgtgaactt ggcgttggtc agcaccagcg gatattccgc ggcgatgtcc
     2101 ggccgactga ctggactcaa ggcaggctcg atgtattccg gcagcgccgg ataaccgtgc
     2161 gcggcaaagg tttcgcagta aagctcgacc ttcttcgatg gtgtagcaaa accacgctgc
     2221 gtgccatctt tgttacgtgc ggcgtatttt tgaaaagccg gctgcgccgg cagtgagatc
     2281 ccgccgggtg actgcttgag gtgggctaaa gtcactccgg tgggttcgag ctcgtaggca
     2341 taagcggcat cgacatcacc atcccagaat ttttcgccga agcccagccg tttggccaat
     2401 tcaaaaataa tccaggtatc ggagcgccgc tcgcccgccg gttttaccac cgcgggccgg
     2461 tactgcatgt gagtcttgcc cgaggggcga tgttcaaagc tcgtggtaat acttgccatc
     2521 tccaggaaac tcgtagccgg caacacgtaa tcgcaaagct ccgctgtggg cgtcataaag
     2581 agatccgccg cgacgccgag ctccaaggcc atcatcgcct cgcgggcgcg ccgcgagttc
     2641 gccgtggaga ggactgtgtt ggagccgaag ttgagaaacg ctttgacggg ataaggctgc
     2701 tgttcgagaa tcgccgtgaa aatatcgtag gccgtgcagt tgccgggctt ggccggcgga
     2761 ccaagcggtt ttttctcgcg cccgatacgg atggctgcca tctccttggg caaaaattct
     2821 ttgccgccga catcattgac cttcaagccg gggaaaataa cgttacctcc ctggcggtca
     2881 tagtcaccga gaagagcgta gagcgaagcg atggcacggc tggtctgcgt cgcgttggta
     2941 tgctggccga gcccgttcca catgtacatg ctgaccggtc gattgtgcgc cagtagcaaa
     3001 gcggtccgcc aaatgtcttt cgctggcacc caggtgatct tttccgagcg ctcgggcgca
     3061 tacgcggaag cgatctcgcc gagtttttca aagaccggtt tgcatgcgat ttcttcgccc
     3121 gactttactc tcaatacacg tgggccgaac agcgccggtt gagtattgtc acgttcatag
     3181 ccgccggtgc ccggatcata gacgacggct tgattcttta actcatccca tacgagatat
     3241 cgat
//
LOCUS       DASUTG010000128         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_20494, whole genome shotgun sequence.
ACCESSION   DASUTG010000128 DASUTG010000000
VERSION     DASUTG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20494"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..374)
                     /locus_tag="VF089_02820"
     CDS             complement(108..374)
                     /locus_tag="VF089_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172920.1"
                     /translation="MEGFLAPKETLTRKRVEEILREKDYSYFEQKVTLLEFRDDSLLL
                     KISFLMKYKNRMIVIEEKEPIVLSFSDFKVEDWGDGIRKIADIV"
     gene            complement(450..881)
                     /locus_tag="VF089_02825"
     CDS             complement(450..881)
                     /locus_tag="VF089_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6172921.1"
                     /translation="MKANSMKVFDVMSKDPLTVSPSDTVGQAEELMYENHYRQLPVVE
                     NKQLIGIVTDRDIRSFLAHSSLLEPEKRGRALRTKVTDIMTTKPLTVSPEEDLREAVE
                     LLIEEKIGGIPVIDEAQGLVGIVTYVDVLRVFLNRLEDEQA"
     gene            1134..1595
                     /locus_tag="VF089_02830"
     CDS             1134..1595
                     /locus_tag="VF089_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6172922.1"
                     /translation="MEPVKKILAPTDLSDISSAGLRYALEMGRSRSAEVIVYHVIDLG
                     DHWIKREDSGPVREMLERHSRMLNKFLWEKFREEMVVTEVRQVVEFGGAVDNIVEKAE
                     GERVDRIVMSTHGRTGIEHLLIGSVAEKVIARAPCPVLIVPAHERNIARAA"
     gene            complement(1607..2422)
                     /locus_tag="VF089_02835"
     CDS             complement(1607..2422)
                     /locus_tag="VF089_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX6172923.1"
                     /translation="MSEIFGFDAYAPKEIAERVETIGVTKARLPLLSQATLGVLAGGF
                     IGLGALYFTFITADASLGFALSRILGGIAFSLGLILVVVAGAELFTGNNFMVIAWASR
                     RISSWELMRNWLIIYCANFTGALGLAVLVFLSNHPEAGAGSVGENAVKIAYAKAALPF
                     AEAFFKGILCNILVCLAVWLAVAGRSVVDKIFAIVFPISAFVAAGFEHSVANMYFIPL
                     GILLRDGSDVANADLLTWGALGSNLVPVTLGNIFGGGVMVALVYYFVYRHHAR"
     gene            2971..3297
                     /locus_tag="VF089_02840"
     CDS             2971..3297
                     /locus_tag="VF089_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6172924.1"
                     /translation="MADEKDRFGDTMRLVERAKEDIYFAEQDRQVLEKLRSQLRKVET
                     VEAPRNCPKCPGKLQGYTFEGYVLDRCHECGGIWLDRGELEAIVKKIRRGPLGGWLDT
                     LTEKIK"
     gene            3312..3830
                     /locus_tag="VF089_02845"
     CDS             3312..3830
                     /locus_tag="VF089_02845"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX6172925.1"
                     /translation="MRSAPKKYNTLYKKGHVERDMYLPRLDPKGVIQCSGCGAFHHRR
                     YWTLTPPPGLSSQMHSHPVFCPACRKIQDNFPGGELNLRGVEAENRQEIARILRNEES
                     QAREKNPLERIMRMDAANGGWRIETTTEKLAQRLGRSIKKARGGKLAYTWGHNNKFVR
                     VVWEKSGMGGAK"
     gene            3842..5764
                     /gene="ftsH"
                     /locus_tag="VF089_02850"
     CDS             3842..5764
                     /gene="ftsH"
                     /locus_tag="VF089_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6172926.1"
                     /translation="MEKQAQDLSIAYIIAIMALMFIAQYFLIAKSVETLSYSQFKTLL
                     KNNQVSDIVIREKYINGSIKPEAVKDILAPQRINELGERAKKPVPFEVVRVEDPRLML
                     ELEGAGIHFKGETTSDWLSVALSWLMPIVIFFVLWSLLSNRMGRAGGGLMQIGKSKAK
                     VYIEKTTGVTFADVEGIDEAEEELIEIVEFLKHPEKYQRLGGHLPKGVLLVGPPGTGK
                     TLLARAVAGEAGVPFFSISGSDFVEMFVGVGAARVRDLFAQAAANAPSIIFIDELDAL
                     GKARGINMLTSHDEREQTLNQLLAEMDGFDPNKNVIMMAATNRPEILDPALLRPGRFD
                     RQVLVDRPDVNGREKILLLHAKKIKLAPSVDLGLIAAKTPGFVGADLANIVNEAALLA
                     ARQSKDAVETADFDEAIERVVAGLQKKSRVMNPREKQTIAYHESGHALTAELVPGADP
                     VSKISIIPRGIAALGYTQQTPTEDRYLMTRSELLARIHVLLGGRVAEEMVFNDVSTGA
                     QNDLQRATEIARTMVTQFGMSDKLGLVSLEGPRTPLFLPVSVQSAKDYSDDTARLIDA
                     EVKQILTDTHHKVKEILATHRAALEELGQLLLKKEVVDRPELQAILKVVRIEQVRGKK
                     KSGDATECEVPASMNN"
BASE COUNT         1527 a         1584 c         1764 g         1348 t
ORIGIN      
        1 gagatttcgc cgagcatgaa cggcaactga gtgaggcgac cgaggaataa ttgcggctcg
       61 aaacccacgg tatcagagaa tacaaagtac tcgtcaactc taaattgcta aacgatatcc
      121 gcgatcttcc tgatcccgtc cccccaatct tcaactttaa agtccgagaa cgaaagcacg
      181 atcggctctt tttcctcaat cacgatcatt cggttcttgt acttcatgag gaacgaaatc
      241 ttgagcaata acgagtcgtc gcgaaattcg agaagcgtga ccttttgctc gaaataggag
      301 tagtccttct cgcgcaatat ctcttctact cgcttgcggg tcagtgtctc tttaggagct
      361 aaaaagcctt ccattgttgt ccctccacat tcctcgtcgc cgatgttctg gagtctaagt
      421 ctcgtatatc gcttctttca gggtgtccgt catgcctgct catcttccaa gcggttgaga
      481 aaaacccgca ggacgtcaac ataggtgacg atgccgacca ggccttgggc ctcgtcgatg
      541 accgggatgc cgccgatctt ttcctcgatc agcaactcga ccgcttcacg aagatcctcc
      601 tccggggaca ctgtcagcgg cttggtggtc attatatcgg ttaccttcgt tctgagcgct
      661 ctgccgcgtt tctccggctc cagaagcgag gagtgagcaa ggaaagagcg aatatcgcga
      721 tccgtgacga tgccaatgag ctgtttattt tcgaccacgg ggagctgtcg atagtggttc
      781 tcgtacatta gctcttccgc ctgacccact gtatcagagg gactcactgt cagaggatcc
      841 ttggacatca catcgaatac cttcatcgag ttagctttca tgatgattca ccccatcacg
      901 atctgtagag agcacaggtt gtgccaagca cgtgggaaaa atgttccggt gaatgtcatc
      961 cgtaccggca tcggtcgcca aacttcgctc ctatgagaaa tacttgccac aaattctatc
     1021 cgatgcgaag cagaaaactt ccaattaagg aatggatagt ttccgatctc accagcggca
     1081 tagttattgc cactgtttgt cataacttcc gagtcgaatt ttcaggaggt catatggaac
     1141 cagtaaagaa aattctagcg ccaacggacc tctcggacat ttcaagcgca gggttgcgct
     1201 atgcgctgga gatggggcga tcacgcagcg cagaggtgat tgtctatcat gtcatcgact
     1261 taggtgatca ctggatcaaa cgcgaggatt ccgggccagt ccgggaaatg cttgagcgcc
     1321 atagccgcat gctgaataag tttctttggg agaagttccg ggaagagatg gttgtaaccg
     1381 aagtccggca ggtggttgag ttcgggggcg ccgtcgacaa tatcgtcgaa aaggccgaag
     1441 gtgagcgggt ggacaggatc gtaatgtcaa ctcacggtcg aaccggcatc gaacacttgc
     1501 tgattggtag cgttgccgag aaagttatcg cacgagcccc atgtccggtg ctgatcgtgc
     1561 cggcgcacga gcgtaacatc gcgagggctg cctaattcgg tgcagctcat cgggcgtgat
     1621 ggcggtagac aaaataatag accaacgcta ccatcacgcc gccgccaaaa atattcccca
     1681 atgtcacggg caccaagtta gagcctaagg cgccccatgt cagcagatct gcatttgcca
     1741 cgtcagatcc gtcccgtaat agaatcccca gagggatgaa atacatgttc gctacgctat
     1801 gctcgaagcc tgcggcgacg aacgcagaga tcggaaagac gatagcgaat atcttatcga
     1861 caacactgcg gccggcgacc gccagccaga cggcaagaca caccaggatg ttacacaata
     1921 ttcccttgaa aaacgcctca gcaaaaggaa gtgccgcctt cgcataagct atcttaaccg
     1981 cgttttcccc taccgatcct gcaccggctt ccgggtgatt cgacaagaag acgagaaccg
     2041 caagtcccaa cgcgccggtg aaattcgcgc agtagatgat cagccagttg cgcatcaact
     2101 cccaagagct tatgcgccga ctcgcccagg cgatgaccat aaagttattg ccggtgaaaa
     2161 gttcggcgcc ggcgaccacg accagaatca aaccgagcga aaaggctata ccgccgagga
     2221 ttcggctaag cgcaaaccca aggctagcgt cggcggtgat gaacgtaaag taaagcgccc
     2281 cgagtccgat aaacccgccg gccagcacgc caagcgttgc ctgtgaaagc agcggaagcc
     2341 gcgctttggt aacgccgatg gtttcgaccc gctcggcgat ttccttgggc gcataagcat
     2401 cgaaaccgaa gatttcactc atgtcgaaat tcttctgaga gtcgctgcaa accgcacccg
     2461 cgggtatttt atcccgcgcg tcagcatgga tttgtcgcaa tagctgttag ccacgttgtt
     2521 catcatacct ccggattagc aaaggtcgtg aagagtcctc cgggtctggc ggttgtatcg
     2581 cggcgcgccg gcagaacgtg ttcccgaaag cggtgttccg accgtcagct cagttaggtc
     2641 tttcgccggc gagctcgagg gcctggtgaa aagcgtggtc gtcatctcgg tccggtctat
     2701 tttctactcg aagcattcga ctagtggaag gtaacccaat tagccaaacg gcaaaagaga
     2761 tgcccgtgag aactttgcga actgcgagga tttctcaatg tcgcgaaaca agtcgccgat
     2821 gtttagcgat tctgataatc ttgcctaccc cgcccgtacg cactctagat atatcgtctt
     2881 cttacaactc aacaaaaagt atttgggatc ctacgcctgc tggcatcaaa ggtgcgttgt
     2941 ttgctggtga aggctttgga ggtgagagat atggcagacg aaaaggatcg atttggcgat
     3001 acgatgcgac ttgttgagag agcgaaagaa gatatttatt tcgctgagca agatcgacag
     3061 gtgctggaaa aactccgcag tcaactgcgt aaggtcgaga ctgttgaagc accgcgtaac
     3121 tgtcccaaat gccccggcaa gcttcagggt tacacgttcg agggatacgt gctcgatcgc
     3181 tgccatgaat gcggtggaat ttggttggat cggggtgagc tcgaggcgat cgtcaagaag
     3241 attaggcgcg gcccgctcgg cggatggttg gatacattaa cggaaaaaat caaatagcga
     3301 ggagctttga aatgagatca gcgccgaaaa aatacaacac cttgtacaag aagggccatg
     3361 ttgaaaggga tatgtatctg ccgcggctgg atccgaaggg tgttatacag tgcagcggtt
     3421 gtggcgcttt ccaccaccgg cgttattgga ctctgacgcc gccccctggt cttagcagcc
     3481 agatgcacag tcatcccgtt ttttgtcccg cgtgccggaa gattcaggat aattttccgg
     3541 gcggtgaact caatctgcgc ggcgttgaag cggaaaatcg tcaagagatt gcgaggatct
     3601 tgcgcaacga agagtcgcag gcgcgggaga aaaatccgct ggagcggatc atgcgcatgg
     3661 atgcagcaaa tggcggctgg cgcatcgaga cgacgaccga aaagctggcg cagcggctgg
     3721 gacgctcgat taagaaggca cgcggtggaa agctcgctta cacgtggggc cataataata
     3781 agtttgtccg tgtggtgtgg gaaaagagtg ggatgggtgg ggctaaatag ggccgagcgt
     3841 cgtggagaaa caagcacaag atctgtcgat cgcatacatc atcgcgataa tggcgttgat
     3901 gtttatcgcg cagtatttct tgattgccaa gtcggtggaa actctttcct acagccaatt
     3961 caaaactctg ctcaagaata atcaggtcag cgatatcgtc attcgcgaaa agtacattaa
     4021 cggctcgatc aagcccgagg cagtcaaaga cattcttgcg ccgcaacgaa tcaacgagtt
     4081 gggcgagcgc gcgaaaaagc cggtgccgtt tgaggtcgtc cgggtcgagg accctcgttt
     4141 gatgttggag ctcgaagggg cggggattca tttcaaggga gagaccacaa gcgattggct
     4201 ttcagttgcc ctgtcctggc tcatgccgat tgttatcttc tttgtcttgt ggagcctctt
     4261 atccaaccgc atgggcaggg ccggcggcgg cttgatgcag atcggcaaga gcaaggcgaa
     4321 ggtgtatatc gaaaaaacca ccggcgttac attcgccgac gttgaaggca tcgacgaagc
     4381 agaagaggag ctcatcgaaa tcgtcgaatt cttgaagcac ccggagaagt atcagcggct
     4441 cggcggtcac ctgccgaaag gagtgctgct ggtggggccg ccaggtacgg gtaagacgct
     4501 gctagcccgg gccgtggccg gcgaggccgg cgtgccgttc ttcagcatct ccggctccga
     4561 ctttgttgaa atgttcgtcg gcgtcggcgc ggcgcgtgtg cgagatttgt tcgctcaagc
     4621 cgcagcgaat gcgccgagca tcattttcat cgacgagctt gatgcgcttg gaaaggcgcg
     4681 cggcatcaat atgctgacca gccacgacga acgcgagcag acgctcaacc agctgctcgc
     4741 ggagatggat ggctttgatc ccaacaaaaa tgtgatcatg atggctgcaa ccaatcgacc
     4801 ggaaattctc gatcctgcgc tgttgcgccc cgggcgattt gatcgacagg tgctggtaga
     4861 ccggccggat gtgaacggcc gtgagaaaat tctgctgctt cacgctaaga agatcaaact
     4921 ggcgccctca gtggacctcg gcttgatcgc ggcaaaaacc ccgggtttcg ttggcgccga
     4981 tttggcgaat atcgttaacg aggcggcgtt gttggctgcg cggcagagca aggacgccgt
     5041 ggagacagcc gattttgatg aagccattga gcgggtggtt gcgggcctgc agaaaaaaag
     5101 ccgggtcatg aacccgaggg aaaagcaaac tatagcatac catgagtccg gtcatgcctt
     5161 gacggcggag ctggttcctg gggccgatcc ggtgtccaaa atatccatca tcccgcgcgg
     5221 catcgccgct ttgggttata cgcagcaaac accgaccgaa gatcggtatt tgatgactcg
     5281 gtcggagctc ctggcgcgca ttcatgtgct gctcggtggg cgcgttgccg aagaaatggt
     5341 tttcaacgat gtctcgaccg gagcgcaaaa cgatttgcag cgggcgacgg aaattgcccg
     5401 gacgatggtt acgcagttcg gcatgagcga taaattggga ttggtctcac tcgaaggacc
     5461 gcggacgccg ctattcctcc ctgtgtcggt tcagagcgca aaagattaca gcgatgatac
     5521 ggcgaggttg atcgatgccg aggtcaaaca gatccttacg gatacccacc acaaagtgaa
     5581 agagattttg gcgacgcatc gcgcggctct cgaagagctt gggcagctgt tgttgaagaa
     5641 ggaagtagtt gaccggccgg agctgcaggc gattctcaaa gtcgtgagga tcgagcaggt
     5701 gaggggaaaa aagaaatcag gtgacgcgac ggagtgtgaa gtgcctgcgt cgatgaataa
     5761 ttgatggacg acactgtcta cggcaagctc gtgatcacga acttccgagc ttgttacccc
     5821 ttctcgagcg gcacgcaaac tactggtaag ggtttcggta gtaatttgat ttcgcaacgt
     5881 agtaggactg gcgccggaaa tctatctata ccaactaatg gcgggatccc ggcgctaacg
     5941 aaacattctt catgaacaat gaatccccaa tagagcaata attcgttccg ccgccacagg
     6001 cggaatgttt ctctcggaac acatagccaa cggagttagc aaacgcggtg gcagctcaaa
     6061 ggactggcac ggccattgcg cacaaaaaac taccatgaag aacaaacaca cgaagctgac
     6121 tgtccttggc ttagctgttt tggcattcgc gacgttcccg cggacgcttc attcctacga
     6181 ggaaatcgtt gtcaaagacg gagcgaacat tcgcggtgcc gtc
//
LOCUS       DASUTG010000129         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1502895, whole genome shotgun sequence.
ACCESSION   DASUTG010000129 DASUTG010000000
VERSION     DASUTG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502895"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VF089_02855"
     CDS             complement(<1..999)
                     /locus_tag="VF089_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172927.1"
                     /translation="MIELRSVGIPSPRVESEQKVGGNAVYAGDVALPGMLWVKVLRSP
                     VAHARITKIDVSRALAVPGVKTVLTGNDFGGARIGKKIIDMPILADGVVRYIGEKVAA
                     VAAESEIAAEAALDLIDVEYDELPIILDPLQAMEPSAPLLHPDLIEYKGLLHKIESPS
                     NVFVHLTWKKGEVEDGFRQSDIIVENTYTVPAVHQAYIEPHCCLAKVNTDGSAEIWAS
                     TKSPFNLREQVGNALQILPSSLIVHPCYVGGDFGGKGDANEVALCYALARKSGRPVKL
                     LIDYSEELASGNPRHGAVIRVKTGVKKNGMMIAQRIEFVFDSGAYGSYRPQGYLVGA"
     gene            complement(1291..>2574)
                     /locus_tag="VF089_02860"
     CDS             complement(1291..>2574)
                     /locus_tag="VF089_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6172928.1"
                     /translation="LLSLEGTGMVLRGRFTPSNGSDGEVEWCDRVLLARIHRLTLGRM
                     RREIEPVSPVEFMSFLLSWQHVGVNRQLEGRDGVLRVIQQLQGLELPAPAWEQWVLPA
                     RVRNYSPADLEHLCLAGVVAWGRLRPEQSSVENLPEKLPGRRRRKRLAVPARNAPIAF
                     LLREDLDYFLGHRSVRWVDVTTLSNSARDVARYLEQRGASFVADIARGAGLLKIKVEE
                     ALWELVAHGLATGDGIAGLRVLLLPEHKRQGKRHNLRVISGGKSAVRMMPVGRWSLWS
                     SQNGAAEVNSEQILEWQARQLLERYGVVFRDLVAREPNLPPWRTLLATYRRLEARGEI
                     RGGRFVNGFIGEQFALPVAIEKLRAERRADDKQPPLIISAADPLNLVGILTPGGRVSP
                     YSNQVIAYRKGVPVQIGSLGSVLSSLQLNQAGEKP"
BASE COUNT          564 a          784 c          670 g          556 t
ORIGIN      
        1 agcgccaacc agataaccct gggggcgata cgagccgtag gcgccgctgt cgaagacgaa
       61 ttctatgcgt tgagcgatca tcatgccgtt cttcttgacc ccggtcttaa ctcttatcac
      121 tgcgccgtga cgcggatttc cggaggctaa ctcttcgctg tagtcaatca gcagcttgac
      181 cggccgccca gattttctcg ccaatgcata acaaagcgcg acttcattgg cgtctccctt
      241 gccgccgaag tccccgccga cataacatgg gtgcacgatc aaacttgatg gaaggatctg
      301 caatgcgttg ccgacttgct cgcgcaaatt aaaagggctt ttggtcgaag cccaaatttc
      361 tgcgctaccg tcggtattca cttttgccaa acaacaatga ggctcgatgt aggcctggtg
      421 taccgccgga accgtatagg tgttctcgac gatgatgtcg gactggcgga aaccatcctc
      481 cacttccccc ttcttccaag tgagatgcac gaaaacattg ctcggcgatt cgatcttgtg
      541 tagcagcccc ttatactcga ttaagtccgg atgcagcagg ggtgccgaag gttccatggc
      601 ctgaagcgga tcgagtataa tcggcagctc gtcatattcg acatcgatca aatccagtgc
      661 tgcttccgcc gctatttcag attcagcggc cacagctgca accttttcac cgatgtaacg
      721 cacgacgcca tcggcaagga tgggcatatc gataatcttc ttgccgatcc ttgcgccgcc
      781 gaaatcattc cctgtgagta cggtcttaac tcctggtact gcgagcgccc gcgatacatc
      841 aatttttgtg atgcgtgcgt gagcgaccgg gcttcgcagc actttcaccc atagcatgcc
      901 gggtagagcc acatcgccgg cgtaaaccgc gttgccgccc actttttgct cgctttcaac
      961 ccgcgggcta gggatgccga cactacgtag ctcgatcata atgacctcat cgatttcttg
     1021 cttgctggaa gatcagcgtt tagacgacga tttgaattta cgctagattc gttggccacg
     1081 ctgaataatc gcgtttttcg gtgaacaata agttgaccgc tacattcaag tcaacactaa
     1141 taggagacag agtggatcct gaattagatt attgctcgtt cggaatgggt tgccatactc
     1201 cgcgaagcag cgcgaactac agcctcctgt caaaaccata atcgagtgcg gagtttttat
     1261 aaccgatcag ccgcccgagc ttctacaggt tcacggcttc tcgccggcct gatttagctg
     1321 caaactactg aggacgctac caagagaccc gatctgaacc ggcacgcctt tacggtaggc
     1381 gatcacctga ttcgaatagg gcgaaacccg tccgccgggc gtaagaattc ccactaggtt
     1441 cagggggtcc gcggctgata tgatgagcgg tggctgtttg tcatcggcgc ggcgttcggc
     1501 ccgaagtttc tcaatagcaa cgggtaaggc aaattgttcg ccgatgaaac cgttaacaaa
     1561 gcggccgccg cgaatctcgc cgcgcgcttc gaggcgccga taggtcgcga gcaaagtccg
     1621 ccatggcggc agattcggct cgcgggcgac aagatcacga aaaaccacgc cgtacctttc
     1681 gagtagttgc cgcgcttgcc attcgagtat ttgttcgctg tttacctccg cggcgccgtt
     1741 ctggctactc cagagcgacc acctgcccac gggcatcatc cgtaccgcgc tttttccccc
     1801 actaatcacc ctcagattgt gacgcttccc ctgccgcttg tgctcaggca gcaacagcac
     1861 tcgcagaccc gcgatgccgt cgccggtagc cagaccatgg gcgaccagtt cccacagcgc
     1921 ttcctcgact ttgattttca gaagcccggc gccacgcgcg atatcggcga caaacgatgc
     1981 gccccgttgt tctagatagc gcgccacatc gcgcgcgctg ttcgaaagcg tggttacgtc
     2041 gacccaacgc accgagcgat gaccgagaaa ataatccaaa tcttctcgca gcagaaaagc
     2101 gatcggggca tttcgcgccg gaactgccaa gcgcttgcgc cgccgcctgc cgggaagttt
     2161 ctccggaagg ttctccacgg aactttgctc ggggcgcaat ctgccccaag ccacgacacc
     2221 ggcgaggcag agatgttcca gatccgccgg cgagtaattc cgcactcgcg ccggcagaac
     2281 ccattgctcc caggcgggag ccggcagctc cagtccctgc agctgttgaa tcacgcgcag
     2341 gactccgtca cgcccctcta gttggcgatt gactccgacg tgctgccaag acaatagaaa
     2401 actcataaat tccaccggcg aaaccggctc gatctctctg cgcatgcgcc cgagagtgag
     2461 gcgatgaatg cgcgccagca ggacgcgatc gcaccattcg acttcaccgt ccgaaccgtt
     2521 cgatggagta aaccggccgc gcaaaaccat gcccgttcct tccagggata acaa
//
LOCUS       DASUTG010000130         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1206267, whole genome shotgun sequence.
ACCESSION   DASUTG010000130 DASUTG010000000
VERSION     DASUTG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206267"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF089_02865"
     CDS             complement(<1..187)
                     /locus_tag="VF089_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172929.1"
                     /translation="MKRLLSLLVISGAVLAALFFLTSLPGNGSGGSPASTVNLQPASS
                     VQQGVLVSIEYTLRDEAG"
     gene            472..1332
                     /locus_tag="VF089_02870"
     CDS             472..1332
                     /locus_tag="VF089_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6172930.1"
                     /translation="MSSEIWAIVSAAAWAADSILVRKGTAFSNPSTAALISFCVNAAV
                     LVPYIIYQYPAEKIFHSANLFFVLSGIIQPAIVRVMFYVGIVRLGVSRAGPIRGTSPF
                     FSVAIAFFLFRERPELIVYLGGLLTIAGTWLVSYKRVGEAKWRAIDLLFPLGAAMLAS
                     VSQNIRKAGLNTTNEPVIASTITTATSLVCLLGSLLFSGKARAIKIDRQSLPFYGGAA
                     VFALIGQLCTFIALNGGQISVVAPLINTTPLFAIAFTALFLRGEEKITPMVLIGVVLL
                     VAGIGFITAR"
     gene            complement(1393..1824)
                     /locus_tag="VF089_02875"
     CDS             complement(1393..1824)
                     /locus_tag="VF089_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6172931.1"
                     /translation="MADDNKSIKKPIPEITPVNQPFWDGTKAGKLMIQKCQDCGTWVF
                     CPRPVCGDCASDKLEWVEVSGKGKIFSFTVIREVVGQALRGFAPDIPYVTAWIDLDEG
                     PRFCSNIVGCPIDQVHIDMPVRVVFEDSGQGVTLPKFMPAD"
     gene            complement(1837..>2310)
                     /locus_tag="VF089_02880"
     CDS             complement(1837..>2310)
                     /locus_tag="VF089_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6172932.1"
                     /translation="YLLGWGECNNSSYGPRYRANPLITGIEQAGKRAFAMAGVTHKDI
                     RCLNLYDDYIPIVMIQIEDLGFCGKNDKAFFEKTDFTFKGDLPIQTSGGMINCGQPST
                     TGGMLHVIETVRQIRGEGGDRQVPNIKFGISTGVGAVNYGKNFGCTAAAILGSES"
BASE COUNT          489 a          643 c          612 g          566 t
ORIGIN      
        1 taccggcctc atccctcagg gtgtattcaa tggacacgag aacaccctgc tgaacgctgc
       61 tcgcgggttg caaattgacg gtcgaagccg gcgagccgcc acttccgttt ccgggcaggc
      121 tcgtaagaaa aaacagcgcg gccaatacgg cgccgctaat gactagtagg ctaagaagtc
      181 ttttcatgga ttggcggcaa ggaacgacaa ttcgccaaag atataatgcg ccagagccga
      241 atacaactag acaggctgct gaaaaacact cataggcttc gttgcgtctt gcccgactcg
      301 cttcaacgta ctgaagcgta cgcctcagct catcgatctt cgcgcgcctc gctctgagtc
      361 tttttgagta gcctgcctgc agaattagca actttaagaa aagcaggtcc tactgtaaca
      421 atcgtaatcg tgctaaaacc tgcgccaaca acatgaaagc ttcaatttag cgtgagcagc
      481 gagatatggg cgatcgtgtc agcggcggca tgggcggcgg attctattct tgtgcgcaag
      541 ggcaccgctt tctccaatcc gtcaacggcc gcgctgatca gcttttgcgt taacgcagcg
      601 gttctggtcc cctacattat atatcagtat cccgcggaga agatttttca ctccgcgaac
      661 ctgttcttcg tcctgagcgg aatcatccag ccggccattg tgcgggtaat gttttatgtc
      721 ggcatcgtcc ggctcggcgt ttcccgcgcc ggaccgattc ggggaacatc gccgtttttt
      781 tccgtggcaa ttgctttttt cctgtttcga gaacgaccgg agctgatcgt ttatttgggc
      841 gggcttctaa ctatcgccgg cacgtggttg gtatcataca agagagtggg ggaagcgaag
      901 tggcgcgcta tcgatctttt gtttccgttg ggcgcggcga tgctagcgtc ggtatcgcaa
      961 aatattcgca aagccgggct taacacgacc aatgagccgg tcattgcctc gaccatcacg
     1021 acagcgacgt cgctggtgtg cctgcttggc tccctgctgt tctccggaaa agctcgagcg
     1081 ataaagattg atcgccagag tctccctttc tatggcggcg ccgcagtctt cgcgctgatc
     1141 ggccagttgt gcacgtttat cgccctcaat ggcggccaaa tttcggtggt cgccccgctc
     1201 atcaacacca ctccgttgtt cgccatagct ttcaccgcgt tgttcttgcg cggcgaggaa
     1261 aaaattaccc ccatggttct gatcggggtc gtgctgctag tcgctggaat cggcttcatt
     1321 accgcgcgat aggcgggttc tcgttaacgt gaagttgacc acaatgtgcg gtgaaccatc
     1381 caacaatttg tctcaatctg ccggcatgaa cttcggcagt gtcacacctt ggccgctgtc
     1441 ttcgaaaacg actcgcaccg gcatatcgat atgaacctgg tcaatcgggc agccgacgat
     1501 attgctgcag aagcgcggac cttcatcgag atcgatccag gcagtgacgt agggaatgtc
     1561 cggagcgaag cccctcagcg cctgaccgac cacctctcgg atcaccgtga aggaaaatat
     1621 cttgcctttg ccactcactt ctacccactc gagtttgtcg ctggcgcaat cgccacaaac
     1681 gggtcggggg caaaacaccc aggtaccgca gtcttggcat ttctgaatca tcaacttgcc
     1741 ggctttggtg ccgtcccaga acggttgatt cacaggcgtt atttcaggaa tgggcttttt
     1801 gatcgatttg ttatcatccg ccataggtca ctcctatcat gattcgcttc ccaaaatcgc
     1861 cgccgctgtg cagccgaagt ttttgccgta attaactgcg ccaacgccgg tgctgatccc
     1921 gaatttgata ttcggcacct ggcgatcgcc gccctcgccg cgaatctggc ggacggtttc
     1981 gatgacgtgc aacatgccgc cggtagttga gggttggcca caattgatca tgccgccact
     2041 ggtctgaatc ggcaggtcgc ctttgaaggt gaagtcggtc ttctcaaaga aagctttatc
     2101 gttcttgccg caaaagccga gatcttcgat ctgaatcatg acgatcggaa tgtagtcgtc
     2161 gtagagattc aaacatcgta tgtccttgtg tgtcacgccc gccattgcga aggcgcgctt
     2221 acctgcctgt tcgataccag tgatgagcgg gtttgctcga tagcgtggcc catagctgga
     2281 attattgcat tcgccccagc cgagcagata
//
LOCUS       DASUTG010000131         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1947869, whole genome shotgun sequence.
ACCESSION   DASUTG010000131 DASUTG010000000
VERSION     DASUTG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947869"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>2221
                     /locus_tag="VF089_02885"
     CDS             137..>2221
                     /locus_tag="VF089_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6172933.1"
                     /translation="MHENLTVGPYSLPHWSGGVAGLRSLDLITKIDGSPVSGRAELYE
                     RVRNLPVGSALNYTVGRHSKTVELTISNMMFTWQDWFLSFGVYVFTGLAFFVIGVAPY
                     CFRANSPAALPLSFMVIAVFVWFESTFDFMTYGFVPKEIRLFGMILTPSAGIHLALLL
                     KQGRPVRYTNPFLLVFIYGIAVVLGSLNSLTFYGPADRWIEIFRINYIFTCVGALAFL
                     LIVGLALRKTATALERSRLRVMFVGAVLGFLLPTVATVLTSTFQWAIPYNLAMISTVF
                     FPLSVAYALLKYSLFDLGNTLKAGLTRLSLMVFLLALYAAIFFLVGPSVGIYDKDPLT
                     PLFFSILVVLIFNPVLRGIETLVDRYIYRQEYDPVKAQREISLFLRSLTTAPILANGF
                     LERVASTLGIEKAILIYRSPATKNDLTAATDGIVADINPILNRARFLWRAEQVSGFRG
                     LSRSEVLANPVFEPQRKEWLAMFEPLGSELLIPIVFEQEVRGLISYSSKRGGREYRGN
                     DLSLLGTLTEQLALSLENGIRYEQSEKAKDEYRQLYDDAERAKRKLIENDRIKKDFVA
                     NICHELRTPVSTLIGYSEILLNRELPAETRDILKTMLSNGQDLSNMMDGLLDFSRMDA
                     DAVPNSFEHISVKDLLGGLELMTQRLIRGRPIQFRIKIEDAIETINSDPRKLQQILVQ
                     LLTNALKFTEKGA"
BASE COUNT          490 a          534 c          586 g          611 t
ORIGIN      
        1 gggggacgac atgcgccagc aagcttggca ccggcaaggg ctgctcgtta tctttgctgt
       61 tttcgcgttt gtcatagtca gcggaacagt gcttctctct ctccaatgga tcaacaaacc
      121 ctttcccggg ttcttcttgc atgaaaatct caccgtcggg ccttactctt tgcctcattg
      181 gagcggcggt gttgcagggc ttcgatctct agatcttatc actaagatcg acggtagccc
      241 ggtaagcggg cgggccgaac tctacgagcg ggtgcgcaat cttccggtgg gcagtgcttt
      301 gaattataca gtcggccggc attcaaagac ggtcgaattg accatttcca acatgatgtt
      361 cacctggcaa gactggttct tgagttttgg ggtttacgtt ttcaccggat tggctttctt
      421 cgtgatcggt gtggcccctt attgttttcg agcaaactcg ccggcagctc tgccactgtc
      481 ttttatggtg atcgctgtgt tcgtttggtt tgagagcacc ttcgatttta tgacctacgg
      541 gtttgtcccg aaagagatac gcttatttgg catgattctc acgcccagcg ccggcataca
      601 cttggcctta ctcctgaaac aaggacggcc tgtacgatat acgaatcctt ttctcctggt
      661 gttcatctat ggaatcgccg tggtcctcgg ctctttgaac agccttacat tctatggacc
      721 ggccgatcga tggatcgaaa tatttcgcat aaattatatt tttacctgtg ttggagcttt
      781 ggctttcttg ttgattgtcg gattagcgtt gaggaagacg gcgacggctc tcgagcggtc
      841 gcgtttgcgg gtaatgttcg tgggcgcggt tcttggattt ctactgccta ccgtcgcgac
      901 tgttttaacc agcacgttcc aatgggcgat accttataat ctggcaatga tctcaaccgt
      961 ttttttccct ctttcggttg cctacgcgct tttaaaatac agcttgttcg atctgggtaa
     1021 tacgctcaag gcaggactca cgcgattgtc gttgatggtg ttcctattgg cgctgtatgc
     1081 agctattttt tttctggtcg gaccttctgt cgggatctac gacaaagatc cactgacgcc
     1141 gctatttttc tcgattctcg tggtgttgat cttcaatccg gtgttacgcg gcattgaaac
     1201 tctcgtcgac cgttacatct atcgacagga gtacgatccg gtaaaggcgc agcgcgaaat
     1261 cagtttattt ctgaggtcgc ttacaactgc tccgatactc gccaacggat ttttagagcg
     1321 ggttgcatca acgctcggca tagagaaggc gatcttgatt taccgttccc ccgccacgaa
     1381 aaatgacttg accgctgcaa ccgatgggat agtggcagat attaatccga ttcttaatcg
     1441 cgcccggttt ttgtggagag cggagcaggt gtctgggttt agagggttgt cccgcagcga
     1501 ggtcctggca aacccagtct ttgaaccgca gcgcaaagaa tggctggcga tgttcgagcc
     1561 cctcggctct gaactgttga taccgattgt ctttgagcag gaggtgcgcg gcttgatttc
     1621 ttattcgtca aagagagggg ggagggagta tcgcggcaac gatttgagcc tgctgggcac
     1681 tctgactgag cagttggctc tctcattgga aaacggcatt cgttatgagc agtcggagaa
     1741 agccaaggat gagtaccggc aactttatga cgatgcggag cgcgccaaac ggaagttgat
     1801 tgaaaatgat cgcatcaaga aagatttcgt ggccaacatc tgtcatgaac tacgcacgcc
     1861 agtcagtacc ctgataggtt atagtgagat cctcctcaat cgcgaactac cggccgaaac
     1921 acgtgatata ttgaagacaa tgttgagcaa tggacaagat ttatcgaata tgatggatgg
     1981 cctactggac ttttcgcgaa tggacgccga tgccgtaccg aacagtttcg agcacatcag
     2041 cgtaaaagat ctactggggg gactggaact catgacgcag agacttattc gcggccgccc
     2101 cattcagttc agaatcaaga tcgaagacgc catcgagacc atcaacagcg atccgagaaa
     2161 acttcagcag attttggtcc agctgttaac gaatgcgctt aagttcactg aaaaaggagc
     2221 g
//
LOCUS       DASUTG010000132         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1206590, whole genome shotgun sequence.
ACCESSION   DASUTG010000132 DASUTG010000000
VERSION     DASUTG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206590"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..606)
                     /locus_tag="VF089_02890"
     CDS             complement(244..606)
                     /locus_tag="VF089_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6172934.1"
                     /translation="MARIAHIALLTKDTEKLAEFYKKSFGMKEVARSGEGGEHGKAIY
                     VSDGHINLAILPARNRREGIYHFGIAVEDVKGAVETALAAGATGGTAELPKDGRFAET
                     FVLDPVGTRIDLSPGWKV"
     gene            complement(758..1768)
                     /locus_tag="VF089_02895"
     CDS             complement(758..1768)
                     /locus_tag="VF089_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172935.1"
                     /translation="MLLRAFKFWVVYFAAAATVFDATWSSAADKIRVAYVSPSVSQSL
                     PWFARELGILAKYDLAAEVLLITGSPRLVQSLIAGDVDFVFAGVAALIRARARGAEVA
                     ILGASANASSQKIMISKTSKIRRIEDLKGAVIGVSQYGSEADTFARVALAQAGLKPDK
                     DTTIIQLGGHPQVAASLAAGKLEIGVLGGLATLTAERAGARVLTSAQDLKVLSPSGTF
                     ATTRGSIQKNRSVVDRLMRSFVEALHYIKTNRNGTITLLQKYLGGLSAEEAAYLYDEQ
                     VNTLEALPAPNEKAIQAVIDREADPKIRSTSVADVIDASFFKNIEKSGLIEQLYKGKD
                     KR"
     gene            2019..2576
                     /locus_tag="VF089_02900"
     CDS             2019..2576
                     /locus_tag="VF089_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6172936.1"
                     /translation="MPVAWPEKDDEELLALIQHGSHQAFSVLVQRHTDRFYRLAYRYL
                     QIKEAAEDVVQDAFVKLWEDPGRWQAERNIKFTTWFYRVVVNSCLDWQKRKRPVELGD
                     RELLIADDRETPDIAMLHAEKHRVLEKEIAALPERQRIALNLCFDEGLSNQEAAEVMG
                     LKLKALQSLVMRAKTTLKERLKNYL"
     gene            2585..2893
                     /locus_tag="VF089_02905"
     CDS             2585..2893
                     /locus_tag="VF089_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172937.1"
                     /translation="MATHQDEIDEMLKARGIQAASPDLAQRIILKAQITPQVQVFSLV
                     RWIKQTFAELHLPNPAYVLAATLIMGFVLGLNVPSDTATESAEAIQALSFLDPDEGLL
                     "
     gene            2890..3324
                     /locus_tag="VF089_02910"
     CDS             2890..3324
                     /locus_tag="VF089_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX6172938.1"
                     /translation="MNNKLKAAFIASIVLNVLLLGIFFGTLSPGFGRESSRRERLTAE
                     IEKLPEPARSRFRDKMAQLRAADDPLREQMRQARNQAIDLLIAEPFDEAVYDKQLIQI
                     NQLRGQMSKRVADDLKELVRGLPPEQRAAVGEMLKRPAPPPK"
     gene            3446..>3988
                     /locus_tag="VF089_02915"
     CDS             3446..>3988
                     /locus_tag="VF089_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX6172939.1"
                     /translation="MKWMKIINQRPKKLISLLALAGFIAGGWFWYASAARQPEQNGQQ
                     SITITRGTIQEVVTAQGKLEAKQYVDVGTQVSGQIKKFHVDIGDTVKQGQLLAELDPR
                     VYQAQVEANEARLNSLRAQLNQQKAQLTLAEQNLRRNRDLFGVNAVSQQALQESETEA
                     AVARAQVASIQAQIQETESNI"
BASE COUNT          957 a         1086 c         1034 g          911 t
ORIGIN      
        1 tcacagcgac agctttcccc ggggtttctg gcttattcgg gggcacatgc gcaacggaca
       61 gctgctaata aattacacat gttgagctgc gtcagcgcct aaggtgttgg aactgagaga
      121 ctatgctgat gggtatgaaa gttcttagtt tataatgccg ctcgaaagga aacgattgga
      181 cacttgagct ataaggtttc gctatgctct taattcacgt gccgagtgtc acgtgatctc
      241 atcctacact ttccaccccg gcgacagatc gatacgggtg ccgaccggat cgagcacgaa
      301 tgtttcagcg aaccgtccat cctttggcag ctcggcggtg ccgcctgtgg ccccggccgc
      361 cagagcggtt tcgaccgcgc ccttcacatc ctccactgca ataccgaaat gatagatgcc
      421 ctcacggcga ttgcgcgccg gcagaatggc cagattgatg tggccgtcgg atacatagat
      481 cgctttcccg tgctccccgc cttcaccgct gcgcgcgact tctttcatgc cgaaggactt
      541 cttgtagaac tccgccaatt tctcagtatc ttttgtcaga agcgcgatgt gtgcgatgcg
      601 tgccatgatt tgtctccttc tatctctcag atcttcttcg aaactgattt cgtcccacga
      661 ctactaaggc gtcattctga ttgctgaggg ctccggatat gattgcgtga atcgatattt
      721 gcccaaacat ggtcagaaaa cgatcaggta tttactttta ccgcttatct ttacctttgt
      781 ataactgttc gatcagtccg ctcttctcaa tgtttttaaa gaaacttgca tcgatcacat
      841 ccgctaccga tgtgcttcta atttttgggt cggcttcgcg gtcgatcacc gcttgaattg
      901 ctttttcatt aggcgccggg agagcttcca acgtatttac ctgttcgtca tagaggtacg
      961 ccgcttcttc cgcgctcagt ccgccaaggt atttctgcag cagagtaatt gttccgttcc
     1021 ggttggtttt gatgtaatgc aatgcttcaa caaaagagcg catgagccga tccaccacgc
     1081 ttctgttttt ttgaatcgag ccccgcgtcg tggcaaatgt gccggacggt gacaagactt
     1141 taaggtcttg agcactggtg agaacacggg cgcccgcgcg ctctgccgtc aatgtcgcca
     1201 atccaccgag cacgccgatc tcgagcttgc ccgcagccag tgacgcggca acttgcgggt
     1261 gaccgccaag ttgaatgatg gtcgtatctt tgtccggttt taaccccgct tgcgcgagcg
     1321 cgacccgcgc gaaagtgtcc gcctccgagc cgtattgtga aactcctatg acggcgccct
     1381 ttaaatcttc gatgcggcga atcttcgagg tcttgctgat catgatcttt tgactcgaag
     1441 cattggcgga ggcgcctaga attgcgactt ccgctcctct tgccctagcc ctaatcaatg
     1501 ccgcaacccc ggcgaacacg aagtcgacgt cacctgcgat cagcgactga accagcctgg
     1561 gactgccggt gatgagtaaa acctcagcgg ccaaatcata cttggcaaga attcccaatt
     1621 ctctggcgaa ccaaggcaga gactggctaa cgctcggact gacataagcg accctgattt
     1681 tgtccgcggc agatgaccat gtggcatcaa aaacggtggc cgcagcagca aaatatacaa
     1741 cccaaaattt gaaagctcta agcagcatca catcacagcc ttacttgaaa tttatctact
     1801 ttctcccgct tctaagacat ccgctcgccg gctgtcaagt tcgcccaagg ttctgccact
     1861 tcaagattcg gtgtccaccg aagagtttcg gcatctcggt cgtatcatca gttgaggcgt
     1921 aatgttcatc ggcactttgg gtctcagttg gatccatgta tgattgagaa gtcccagcaa
     1981 ttcaccaaac ttcgagagac aactcgaatc acaactctat gcctgttgcc tggccggaaa
     2041 aggacgatga agagctcctt gcgcttatcc aacacggcag ccaccaggcc ttcagtgttc
     2101 tcgtgcagcg acacacggac cgattctacc ggctcgccta ccgctatcta caaattaagg
     2161 aggcagccga agacgtcgtc caggatgctt ttgtcaaact atgggaggat cccggcaggt
     2221 ggcaagcgga gcgaaacatc aaattcacga cttggtttta ccgggtcgtg gtgaattcat
     2281 gtctcgactg gcagaaaaga aaaagaccgg tcgagcttgg tgatcgggag ctgttgatcg
     2341 cggatgatcg agaaacgccc gatattgcta tgttacatgc ggaaaaacac agagtacttg
     2401 agaaagaaat cgctgctctg ccggagcggc agcggattgc actgaatctc tgcttcgatg
     2461 aaggattgag caatcaggaa gccgccgaag tgatggggct caagttaaag gctttacaat
     2521 cactggttat gcgcgccaaa acgacgctta aagaacgtct gaaaaactat ttgtgaggat
     2581 cgctatggcc acccaccaag acgaaatcga cgagatgctt aaagcacgcg ggatccaggc
     2641 cgcaagcccg gacctcgcac agcggattat cttaaaggcg cagataacgc ctcaagtaca
     2701 ggtattttcg ttggtgcgat ggatcaagca aacctttgcc gagctgcatt taccgaatcc
     2761 ggcttacgtc ttggccgcca cgttgatcat ggggtttgtc ctcggtttga acgtcccctc
     2821 ggacacggca accgagagtg ccgaagccat ccaggcgctt agctttctcg atccggacga
     2881 aggactatta tgaacaataa actgaaggcc gcttttatcg catctatagt tttaaacgtt
     2941 ttgttgctgg gcattttctt cggtacgttg agccccggct tcggtcggga gagttcacgg
     3001 cgagagcgcc tcaccgccga aatcgagaaa ctgcccgaac cggcgcgcag ccgattccgc
     3061 gacaaaatgg ctcaactgcg cgccgccgat gatcctttgc gggagcagat gcgccaggcc
     3121 cgcaatcaag cgatcgatct tctgattgct gaacccttcg atgaagccgt gtacgacaag
     3181 cagctaattc agatcaacca actgcgtggg cagatgtcga aacgtgtggc tgacgatctt
     3241 aaagagctcg tcagaggatt accgccagag cagcgtgccg ctgtgggtga gatgctcaaa
     3301 cggcccgcgc cgccacccaa gtaactcgtt tctcttaaat tggcacaaaa gcgctggtct
     3361 taccggcgct tttttttgcc tgaacggcgg agacaaaggg cgcttggttc gtatcactgt
     3421 aaggacaacg aaaggcaacg ccactatgaa atggatgaaa ataatcaacc agcggccgaa
     3481 aaaactgatc agcttacttg ctctagcggg attcatcgct ggcggctggt tctggtatgc
     3541 cagcgcggca cgccagccgg agcaaaacgg tcagcaatcg atcacgataa cgcgcgggac
     3601 aattcaggaa gtggtcacgg cacagggcaa gctcgaagca aagcaatacg ttgacgtcgg
     3661 cacacaagtg tccggtcaga ttaaaaaatt tcacgtcgat atcggcgata cggtgaagca
     3721 aggccagcta ctggccgagc tcgacccgcg cgtgtatcag gcgcaagtcg aagccaacga
     3781 agcccggctg aacagcctga gagctcagct caatcagcag aaagcgcagc tcaccttggc
     3841 ggagcagaac ctcaggcgca atcgcgattt gttcggcgtc aacgccgtca gccagcaggc
     3901 acttcaggaa tccgaaacag aagcggcggt tgctagagcg caggtcgcct cgattcaggc
     3961 gcagatccaa gaaaccgaat caaacatc
//
LOCUS       DASUTG010000133        11250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1355900, whole genome shotgun sequence.
ACCESSION   DASUTG010000133 DASUTG010000000
VERSION     DASUTG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355900"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1542)
                     /locus_tag="VF089_02920"
     CDS             complement(52..1542)
                     /locus_tag="VF089_02920"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6172940.1"
                     /translation="MTALEARKLAEDEIKAGKTPKYTDLRDWLEIVDSFGELKKIDGA
                     DWNLEMGTLAELLSRETKGGAVPAVLFDNVPGYPKGFRTLFAQNASFKRMALNLGLPL
                     ELSNLDLVRAFRQKLVTHEPIKHKVVTKGPILENVNSGDKVNLLKFPVPFMHELDGGR
                     FIGTACLVTTRDPDEGWVNFGAYRGMVHDEKSMGLYISPGKHGSIQRAKYFERGKPCP
                     VVISVGQDPVLFMVSGNEIDYGVGEFDYAGGLKGAPIEIIEGKATGLPFAANAEIVIE
                     GWMDPTDRKTEGPFGEWTGYYASSSRPEPVVRVENIYYRNDPILCCARPGRPPSDYSI
                     AKCMVKAALIWEQVEKCGVPDVKGVWCHEAGGGRLFNIISIKQRYPGHARQAMLAASQ
                     VHAGAYLGRYVIVVDDDIDPTNTFDVIWALASRSDPVESIEILRRCWSGPLDPRIHPS
                     KKGFNSRAVIDACRPFEWMKEFPPVAESSPELREKIYNKWKKVIEA"
     gene            complement(1542..2726)
                     /locus_tag="VF089_02925"
     CDS             complement(1542..2726)
                     /locus_tag="VF089_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6172941.1"
                     /translation="MAEKRNMVQIEALEKYFGEDKERVHVLKGVSLNIPEGLLYTFLG
                     PSGCGKTTTLRCVAGLERPDGGRISIGGQTVFASGERVYVPTNKRPIGMVFQSYAIWP
                     HMTVAQNVAYPLTIQRRSKDEIKRRVKDVLRIVGLDGLEDRPAPKLSGGQQQRVAFAR
                     ALINEPKVMLLDEPLSNLDAKLRVQMRSEIKALQRRTNITTIFVTHDQAEALAISDQI
                     AVMHGGTLIEVGSPHQLYTRPKRRFTATFLGLTNLIDGKVLDVDGDGRPGKMETKKGI
                     LSFIPAPGLKKDQAAVISIRPENIPLHKEKPQSLENVLEGTVTEAIFMGDSYQCKIAV
                     GEDILAVHTHPFQSVNPGDKVYLHLDPKSCNGLPADDTEGVEETMVANYDKESPQAKG
                     VL"
     gene            complement(2780..4471)
                     /locus_tag="VF089_02930"
     CDS             complement(2780..4471)
                     /locus_tag="VF089_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6172942.1"
                     /translation="MRRPNSQTLILIGAIVFVVYLAGVPLLMLLYGSIRSAPIGEPGA
                     TYTIQNYVRAYFDKEFYLLFLNSLYFAVGTCVLTCLIGTFLAWVSERTNTPFKKVFVV
                     MALVPFIIPGILSTISWILLLSPKIGLINLTLKGLLGLESAPFNVYSMWGMIWAEAIH
                     DYPLVFLLMSAALRNMDTSLEEAALTAGSSTWTTFKRVTLPLMRPALFSVLLIVFIRG
                     IEAFEVPALIGVPAKISVFTTKIFLAIHQFPSDFGLAGSYAVTLLVISSIGVLIYGRI
                     TRREERYATVTGKGYRPRVIDLGKWKYFTCALSFFIFFLAVILPVFVLLWSSFIPYYG
                     VPSRELMGKMTLANYQYILNYPLAVTAFKNSFYLSIGSATLVMLLTSVIAWITVRTKL
                     PGRALLDNMTFIPIAMPGIVLGVSLIWVYLTLPIPIYGTMWVLLLAYITKFMPYGIRA
                     ASASMIQINKELEEASLTAGGTWLQTFKRIILPLLMPGFTAGWIYISIIALRELSTSI
                     LLYSYNSTVLSIMAFDLWEGGQYTYVCALGVLMVLLLIMMAFIARKLGAKMGIAQ"
     gene            complement(4495..5310)
                     /locus_tag="VF089_02935"
     CDS             complement(4495..5310)
                     /locus_tag="VF089_02935"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6172943.1"
                     /translation="MAQTKLKAVNLRMEYYQPRTGGRLLALDNVNLQVDEGEFVTIVG
                     PSGCGKTTFINLADGLLKPTGGQLMIDDRPVTGPGTDRGMVFQDSCLMPWRTVFKNVI
                     FGLECQGLDNGEGKERAQKFIKLVGLGGFEDHYPHELSGGMQQRCNLARALTVDPEIL
                     IMDEPFAALDAQTREIMQSELLRIWRESGKTVLFITHQINEAIYLADRVIVFGARPGR
                     VKEMMKIDIPRPRPLSVKRDKRFLEYEDHLWTMIEEEVKKTMVQDQVVHNIDA"
     gene            5504..6274
                     /locus_tag="VF089_02940"
     CDS             5504..6274
                     /locus_tag="VF089_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6172944.1"
                     /translation="MNYPLKQPKTRSRGSENLSARVYNEIKNLILCNEVMPGQKLHHQ
                     QLSERLGVSRTPVREALTRLVQEGYVAFLPNRGFTCKEIRMQEAEELYDLREALEAFA
                     VQKAIENLTEMGLEQLREKMELYGGDVQNRFTRERLMYDQDIHIEIAQLAGNETLKKT
                     LGHVFERIVLKRRTDGLYDAARGVNAHQQHLRLLQAMKQRNVAEAVSIVRAHIRKGKQ
                     NVLSDLEQRLAIRELRATATVEPRENQTKRKEEKWPQI"
     gene            6259..7629
                     /locus_tag="VF089_02945"
     CDS             6259..7629
                     /locus_tag="VF089_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX6172945.1"
                     /translation="MAADLVIKNGWVVTPEETFQGGVAISNEKIVAIGANDVLPQGKE
                     EVDAKGKHILPGIIDGHVHFREPGMTHKEDFATGSTAAVCGGITSVVDMPNTVPPTAQ
                     AEQVKIKQQLGEAKSLVDFGVTGVVVQTNTKDILPMADAGAIGFKIFFGETIGNLPFP
                     DDGMCMEAFDYIAQSKLPLGIHAENRQIMAYCTNKLKTEGKNEARYWEASRPDICEAE
                     SVHHAIFFAEMFGTKLHVFHMSSKQAAYMVRDAKARGMRVTAETGPHYLLREPNDMDQ
                     VGSLLKMNPPVRTKDHGEVLWQGLREGFVDTLATDHSPHTLEEKGSDIYGKLTKPAIW
                     DCISGFCGVETGVPLILTEVNKGRLTLNQYVKVACENPAKVWQVYPKKGAIKLGSDGD
                     ITIVDMDKEGTIDVNKLHSKNKPSPWHGWKVKGMPVCTVVRGHVQMRDGEPCGKPIGR
                     MLRPLI"
     gene            7769..8680
                     /locus_tag="VF089_02950"
     CDS             7769..8680
                     /locus_tag="VF089_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6172946.1"
                     /translation="MKRTEAFEMYQPTSLREASLLVKEKGPGGRFLAGGTDLVIAMKE
                     KGLVPKYVVDLKRLPGLSGIHENSDGSITIGALTTMREIEISPLLTKKYPFLCQSAAE
                     VGSIQIRNRATIGGNMSNATPSADVAPSLIALQATAKIASARSERSVAIEEFFRGPGL
                     NTMDSDEILTEITIPKTSRRLVGEYIKFSPREMMDLAYVGIAVAYTLGEKENRCSEVR
                     IVLGAVAPTPIRAKKAEAALEGQVLSEDLAEKVGAIAAEEAKPISDVRSSADYRRAMV
                     GAMTKRALLNAMVGPANSWVERRDRRY"
     gene            8755..9234
                     /locus_tag="VF089_02955"
     CDS             8755..9234
                     /locus_tag="VF089_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6172947.1"
                     /translation="MNKKIQFTLNGKSITREVASHRLLLDLLRDEIGLTGTKEGCGTG
                     DCGACTVLMNGKPVNSCLILSGELDDATIVTIEGLKIGPELHAIQKAFIEDGGAQCGY
                     CTPGMLMMSKALLDENPSPSEEDIRFALSGNLCRCTGYAKIIQAVQDAAAMMRAKSD"
     gene            9307..>11250
                     /locus_tag="VF089_02960"
     CDS             9307..>11250
                     /locus_tag="VF089_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6172948.1"
                     /translation="MAELTVVGKPVTRVDASEKVTGEAVYGYDLVLPNMVYGKVLFSP
                     KAHAKIKKINTEKAKAYPGVVAVVTGADAPWTHGESIKDKPFLAQDKVRYIGEPVAAV
                     AAEDEDTAQAAVKLIEVDYEDLPVYVDPEQASKADAIAIHEDFPNYRKAEFIVPGAHA
                     NIAEHFKLRTGDVGEGFQNSDLVLEERYFVPMIQHAAMEPHSAHAQFDKQSGRLTIWV
                     ANDAPFRALNELTEALEMPKEKIRLINPLQGGGFGSKGGLKVEPIAVALAFHTNGRPV
                     RVKFNREETFISTLTRHEAIVYCKTGVKKDGTLMAREMTIYWGAGAYAEKSPTVCIRG
                     SLPAPGPYRIPHVKVDGYAVYTNKPVAGSYRGYGIPQGAWAGEQQMDEIAKRLGMDPV
                     EIRMKNIFVEGDISYWGEQLHAVGLKETLTKAIEAIDWGKKPAQTKPGVKVGKGFACI
                     QKPTRSPTTSAAGVMVNTKGEVTVLAGTVEIGQGCNTILSQVAAEELKVPMEKVHMHP
                     LDTDVIPYDASTTSSRSTYHMGNAVRGAAIHAGEQIMEAASPMLEAKTDDLDCRDGKV
                     FLKDQPQMALTLGEVVRRKLGAEGVVRGDSSYTYEIGKDLDLETGHSDHASAFYMYAT
                     QAAEVEVDEDSGRVRILRMSAAHD"
BASE COUNT         2828 a         2973 c         2999 g         2450 t
ORIGIN      
        1 agagggcgac tgcttatgcg gccgccctcc tgtaagcacc atagaattct tctaagcttc
       61 gataactttt ttccacttgt tgtagatctt ttcgcgcaat tcgggcgagg actcagctac
      121 cggcgggaac tctttcatcc attcgaacgg acggcacgca tcgatgacgg cacgggagtt
      181 aaagcccttc ttgctcgggt gaatgcgtgg atcgagagga ccgctccagc agcggcgtag
      241 gatctcaatc gactccaccg ggtcggaacg gctggccaaa gcccaaatta cgtcgaacgt
      301 gttggttgga tcgatgtcgt cgtcgacaac gatcacatag cggccgagat aagcgccggc
      361 atgcacctgg gaggctgcca gcatcgcttg acgcgcatgg cccggataac gttgcttgat
      421 cgaaataatg ttgaataaac ggccgccacc ggcctcgtgg caccacacgc ctttcacatc
      481 aggaacgccg catttttcaa cctgttccca gatcaacgct gccttgacca tgcatttggc
      541 gattgaatag tccgacggcg gtctgccggg gcgcgcgcaa cagagaatcg gatcattgcg
      601 gtagtaaatg ttttccaccc gcaccaccgg ttccggacgg ctcgagctgg catagtaacc
      661 ggtccattca ccgaagggtc cttcggtctt ccggtctgta ggatccatcc atccttcgat
      721 gacgatttct gcgttggcgg caaatggtag cccggtggct ttaccttcga tgatttcgat
      781 gggcgcaccc ttcagtccac cggcatagtc aaattcgccc acaccgtaat cgatctcgtt
      841 accggaaacc atgaacaaca cggggtcctg accgaccgag ataacaaccg ggcaaggttt
      901 tccgcgttca aaatacttcg cacgttgaat gctgccatgc ttaccgggag aaatgtacaa
      961 gcccatgctc ttttcgtcat gaaccatgcc ccgataggcg ccgaaattca cccaaccctc
     1021 atccgggtcc cgcgtcgtaa ccaggcaagc agtgccaatg aaacggccgc catcgagctc
     1081 gtgcataaaa ggcacgggga acttgagcag attgactttg tcacccgaat tcacgttctc
     1141 caggatcgga cctttggtca ctactttatg cttgatcggc tcgtgcgtta cgagcttctg
     1201 gcggaaggct cgcaccaaat ccaaattcga gagttccagc ggcagaccga gatttaacgc
     1261 catcctctta aatgacgcat tctgggcgaa gagagtacga aatccttttg gatagccagg
     1321 cacgttatca aaaaggaccg ccggaacagc cccgcctttg gtctcgcgcg aaaggagctc
     1381 agcgagcgta cccatctcca gattccaatc ggctccgtcg atttttttga gttcgccgaa
     1441 actatcaaca atctcgagcc agtcacgtag atcggtgtat ttaggtgttt taccggcttt
     1501 aatttcatct tcggctagtt ttctagcctc tagtgcggtc attagagtac tcctttcgcc
     1561 tgtggcgatt ccttatcgta gttcgctacc attgtttctt caacaccctc cgtatcatcc
     1621 gcgggcaatc cattgcaact cttggggtct aagtgcaaat aaactttatc gcctggattc
     1681 accgattgaa atgggtgtgt gtgaacagcg agaatatcct caccgacagc gattttgcat
     1741 tggtaggagt cgcccataaa gattgcttcc gtgacagtgc cctcaagcac gttctccaag
     1801 ctctgcggct tctctttgtg caacggaatg ttttctgggc gaatcgagat taccgccgcc
     1861 tgatctttct ttaacccagg cgccggaatg aaactcaaga tgcctttttt tgtctccatc
     1921 ttacctggcc tgccgtcacc gtcaacgtca aggacttttc catctatgag attggtcaga
     1981 cccagaaatg tcgcagtaaa ccgccgtttc ggtcgagtat aaagctggtg cggcgaaccg
     2041 acttcgatca gtgttccgcc atgcatgacg gcgatttgat ctgaaatcgc caaagcttcg
     2101 gcttgatcat gcgtgacgaa gatcgtggtg atattggtcc gtcgctgcaa cgcttttatc
     2161 tccgagcgca tctgcacgcg caattttgca tcgaggttac tcagcggctc atccagcaac
     2221 ataacttttg gctcattgat cagagcgcgc gcgaacgcaa cccgctgctg ctggccgccg
     2281 gaaagttttg gcgcgggccg atcttccagg ccgtcaagtc cgacaatcct gagaacgtct
     2341 ttgaccctgc gtttgatttc gtccttcgat ctacgttgaa tggtcagggg atacgccacg
     2401 ttttgagcaa ccgtcatgtg cggccaaatg gcataggatt gaaacaccat cccaatcgga
     2461 cgtttgttgg tgggaacgta gacacgctcg cccgaagcaa aaacagtttg tccgccaata
     2521 ctgatcctgc caccgtcggg cctctctaac ccagcgacac agcgcaaggt cgtggtcttg
     2581 ccgcagccgc ttgggcccag aaacgtatag agcaatccct ccggtatgtt cagcgatacg
     2641 cctttcagaa cgtgaacccg ttccttgtcc tcgccgaaat acttttcgag agcctcgatc
     2701 tgaaccatgt ttcttttttc tgccatatct cctccgcaat gatgacggct ctatagattg
     2761 ctcctccaag gtgattaact tattgcgcga tccccatttt tgctcccagc ttacgtgcga
     2821 taaatgccat catgataagc aacagaacca tcaggactcc taatgcacaa acgtacgtgt
     2881 attgcccgcc ttcccacaag tcaaatgcca taatcgaaag aaccgtgctg ttgtacgaat
     2941 aaagtaagat ggatgtggaa agctcgcgta atgcaatgat actgatgtaa atccaccctg
     3001 cggtaaaacc gggcatcaac agcggcagaa tgattctctt gaacgtttgc aaccaggtgc
     3061 cgccggccgt gagcgacgcc tcttcgagct ccttattgat ttgaatcatg gaagccgacg
     3121 ccgctcgaat gccgtagggc ataaacttag tgatgtaagc taacaacaat acccacatcg
     3181 tgccatagat cggaatgggc aatgtgagat acacccatat caagctgaca cccagtacga
     3241 tgcccggcat ggcgatggga ataaaggtca tattgtctaa cagggctcgt cctggaagct
     3301 tagttcttac ggttatccag gcgatcacag atgtgagcag catcaccaat gttgccgaac
     3361 cgatggagag atagaaactg ttcttgaatg cagtcaccgc gagcggatag ttcaatatgt
     3421 actgataatt ggccagcgtc atcttgccca tgagctcacg ggacggaacg ccgtaatagg
     3481 gaatgaacga agaccacagt aacacaaaca cgggcaagat tacggcgaga aagaatataa
     3541 agaacgacag cgcgcaggta aaatatttcc atttgccaag atcgatcacc ctgggacgat
     3601 agcccttgcc ggtaactgtg gcgtaacgct cctcacgccg cgtaatcctg ccgtagatca
     3661 aaacgccaat gctgctgatg actaatagcg taaccgcata ggaccctgcc agtccgaaat
     3721 ccgagggaaa ctgatggatg gctagaaaaa ttttagtggt aaaaaccgag atcttggccg
     3781 gcacgccaat cagcgccgga acttcaaatg cctcgatgcc gcgaatgaag acgatcaaca
     3841 acacactgaa caaggccggg cgcatcagag gcagggtaac tcgtttgaag gtggtccacg
     3901 tactcgaacc agctgtcagc gccgcctctt ccagcgaagt gtccatgttg cgaagggcgg
     3961 cagacatgag tagaaaaacc agcgggtagt cgtgtatcgc ttccgcccag atcatgcccc
     4021 acatggaata aacattgaag ggcgccgact ccaggcccaa gagccccttc agggtcagat
     4081 taatcaaccc gattttcgga ctaagaagaa gaatccatga aatcgtgctg agaatcccgg
     4141 gaataatgaa agggaccaac gccattacga cgaatacctt tttaaacgga gtgttggttc
     4201 gttcgctgac ccacgccagg aacgttccaa tcaaacaggt gagcacgcag gtgccgactg
     4261 caaaatagag ggaattcaga aagaggaggt aaaactcctt gtcgaaataa gccctgacgt
     4321 agttctgaat cgtgtaggta gcaccgggtt cgccgatggg agcgcttctt atgctgccgt
     4381 aaagcaacat aagaagcgga acacccgcca gataaacaac aaaaacgata gcgccgatca
     4441 gaatcagggt ctgggagttt ggacgccgca cccttaactc ctgcaaggct ggcgctatgc
     4501 gtcgatatta tgcacaactt ggtcctgaac catggttttc ttaacttctt cctcaatcat
     4561 ggtccagagg tgatcttcat attccagaaa tcttttgtcg cgtttgacgg aaagcggtcg
     4621 aggacgtgga atgtcgatct tcatcatttc tttgactctg ccaggtcgcg cgccaaacac
     4681 tatcacgcgg tcggccaaat agatggcttc attgatctga tgcgtgataa acagcacggt
     4741 cttccccgat tcgcgccaga tgcgtagcag ctcggactgc atgatttctc gagtttgcgc
     4801 atcgagcgct gcaaacggct catccatgat gaggatctct ggatcaacgg tgagagcgcg
     4861 ggctagatta cagcgctgct gcataccacc ggagagttca tgagggtagt gatcttcgaa
     4921 gccgccaagc ccgaccaact tgatgaactt ctgcgcccgc tctttgcctt cgccgttatc
     4981 gagcccctgg cactctaggc cgaagatcac attcttgaaa actgttctcc agggcatgag
     5041 gcaggaatct tgaaaaacca ttccacgatc ggttcccggt ccggtgaccg gccgatcatc
     5101 aatcatcaac tgtccgccgg tcggcttgag caatccgtcg gcaaggttga tgaaggtggt
     5161 cttgccgcaa ccgctaggtc cgacgatcgt gacaaattca ccttcatcga cctgaagatt
     5221 gacgttatca agcgcgagca gccgacctcc ggtacgcggc tggtagtact ccatccgtag
     5281 attgactgct ttaagtttcg tttgtgccac atggcctcct taaaggttct tttcgtccat
     5341 aactctttaa aactacatta accaccagca gacggacaaa acgtcgtaca tttagcaaaa
     5401 tcaaatcttt tttcaagcgt tgtcccatag attgacaacg ctcagccgat tcggtaggtt
     5461 ccccgaacaa tttgtataaa ttattttatg caaattcgga tcgatgaatt atcccctgaa
     5521 gcaaccgaaa actagatcgc gcggcagtga aaatctttcg gctcgcgttt acaacgaaat
     5581 caaaaatcta atcttgtgca atgaggtcat gcccggtcaa aagctccatc atcagcagct
     5641 cagcgagcga ctcggcgtca gtagaactcc tgttcgcgaa gcattaaccc gcttggtgca
     5701 ggaaggctat gtcgcgtttt tgccgaaccg gggttttacc tgcaaagaaa ttagaatgca
     5761 agaggccgaa gagctctatg atctccggga agcgcttgaa gcatttgcag tgcaaaaggc
     5821 catcgaaaat ctgaccgaga tggggcttga gcagctgcgc gagaagatgg aattgtacgg
     5881 cggcgacgtg cagaatcggt ttactagaga acgactcatg tacgatcaag acattcacat
     5941 tgagatcgct caattggctg gcaatgagac gttgaaaaaa acgcttgggc atgtttttga
     6001 gcggatcgta ctgaaacgca gaaccgacgg gctttatgac gctgcgcgcg gcgtcaacgc
     6061 gcatcagcag catctacggc tgttgcaagc gatgaagcag cggaacgtcg ctgaggcggt
     6121 cagcatcgtc cgggcgcaca tccgcaaagg aaaacagaac gtgctttcag atttggagca
     6181 aaggttggcg atccgggaat tacgtgcaac agcaacggtc gaaccaagag aaaaccaaac
     6241 aaaaagaaag gaagagaaat ggccgcagat ctagtcataa aaaatggttg ggtggtaaca
     6301 cccgaggaga cgtttcaagg gggggttgcc atcagcaacg agaaaatcgt cgcgatcggc
     6361 gctaacgatg tattgcctca ggggaaagaa gaggtcgacg ccaagggaaa acacatcctt
     6421 ccggggatca tcgatggaca cgtccatttc agggaaccag gcatgaccca taaagaggac
     6481 ttcgccaccg ggtcaacggc cgccgtttgt ggcggaatca cctcagtcgt tgacatgccg
     6541 aacaccgttc cgcctacggc ccaagccgag caagtgaaaa taaagcagca gctgggcgag
     6601 gccaagtcac tggtcgactt cggcgtcacc ggtgtcgttg tgcaaaccaa caccaaagac
     6661 attttgccaa tggccgacgc cggcgccatt ggtttcaaga tcttcttcgg tgagactatt
     6721 ggtaatctcc ctttcccgga cgacggcatg tgcatggagg ccttcgacta catcgcgcaa
     6781 tcgaagttgc cgctgggaat tcacgcagag aatcggcaga tcatggcgta ttgcaccaac
     6841 aagctaaaaa ccgagggcaa gaacgaggcc cgctattggg aggcttcgcg cccggacatt
     6901 tgcgaggcag aatccgttca tcatgcgatc ttcttcgccg agatgttcgg caccaagctg
     6961 cacgtcttcc atatgagctc caaacaggca gcctacatgg tacgcgatgc caaagcccgc
     7021 ggaatgaggg tcacggccga gaccggcccg cattacctgc tgcgcgagcc caacgacatg
     7081 gatcaagtcg gctcattgct gaagatgaac ccgccggtgc gaactaagga tcacggtgag
     7141 gttctatggc agggtcttcg tgaaggattc gtcgacacgc tcgccacgga ccattcaccg
     7201 cacacgctcg aggaaaaagg ttcggatatc tatggaaagc tcaccaaacc ggcgatctgg
     7261 gactgcattt cgggtttctg cggtgttgaa accggcgttc ctttgatctt gactgaggtg
     7321 aacaaaggtc gcttgacctt gaaccagtat gtcaaagtgg cttgcgaaaa tccggctaag
     7381 gtttggcagg tttatccgaa aaagggcgcc attaagcttg gctcggacgg tgatatcacg
     7441 attgtcgaca tggacaagga aggcacgatc gacgtcaata aactgcacag caagaacaag
     7501 ccctctccgt ggcacggttg gaaagtcaaa gggatgccgg tatgcacagt ggtccgaggt
     7561 catgtgcaaa tgcgcgacgg cgagccttgt ggtaaaccga tcgggcgcat gctcaggcct
     7621 ttgatctagg tttcccgcag ttaaccctca gcgaacgtcc agcggcacag ctgatggttt
     7681 cagttaatgg tggacttttc tattcgtccc ccgaaactga aacttgagct tgccgctggc
     7741 ggtctttgtg ttgtcaagga agtagccgat gaaacgtacc gaagctttcg agatgtacca
     7801 gccgacttcg ctgagagagg ccagcctgct cgtgaaagaa aaaggccctg gcggccgttt
     7861 tctcgcggga gggaccgacc ttgtaatcgc tatgaaggag aagggtttgg tgccgaaata
     7921 tgttgtcgat ctcaaacggc ttcctggttt gtccggcata catgaaaata gcgacggcag
     7981 cattaccatc ggtgcgttaa cgaccatgcg tgagatcgag atatctccgc tgctcactaa
     8041 gaagtatcct tttctttgcc aaagcgcggc cgaagtgggt tcaattcaga ttcgcaaccg
     8101 ggcaaccatc ggcggcaaca tgtccaatgc caccccgtcc gccgatgtcg cgccaagcct
     8161 gattgcactt caagctacag caaagattgc cagcgcccgc agcgaacgca gcgtagcgat
     8221 cgaagagttc ttccgcggtc ccgggctaaa taccatggat tcggacgaga ttcttactga
     8281 aattacgatt ccgaaaacta gccggcggct ggtcggcgag tacatcaagt tctcgccacg
     8341 cgagatgatg gatttggcat acgtcggtat cgcggtagcg tacacgctgg gcgaaaagga
     8401 aaatagatgt tcggaagtgc ggattgttct cggcgccgtc gctccgaccc cgattcgcgc
     8461 gaaaaaggcg gaagccgcac tagaaggcca agttctctcc gaagacttgg ctgaaaaggt
     8521 tggagcgatc gccgcggaag aggcaaaacc gatcagcgac gttcgctcat cagcggatta
     8581 tcggcgcgcc atggtgggag ctatgaccaa gcgggccctg ctcaatgcga tggtcggtcc
     8641 ggcgaactcc tgggtcgagc ggcgcgatcg tcgatactag acgagtgccg aagaaccgca
     8701 gcgaatatcc cttgatcaac tccatcggtt cgattttgtt taaatgaggc cgtcatgaac
     8761 aagaaaatcc aattcacgct aaacgggaaa agcattacaa gggaagttgc cagtcatcgg
     8821 ctattgctcg acttgctccg cgatgaaatc ggccttaccg gaaccaagga aggctgcggc
     8881 accggcgact gcggcgcttg cactgtgctg atgaacggca aacccgtcaa ttcgtgcctg
     8941 attctctccg gcgaactcga cgacgcaacg attgtcacga ttgaaggcct taaaatcggc
     9001 cctgagcttc acgcgattca aaaagcattc attgaagatg gcggcgcgca gtgcggatat
     9061 tgcacgccag ggatgttgat gatgtcgaaa gcgttgctcg acgagaatcc ttcccccagt
     9121 gaagaagaca tccgcttcgc tctctctggc aatctttgcc gttgcaccgg atacgccaag
     9181 atcattcaag ccgtccaaga tgcggcagcg atgatgcgcg ctaagagcga ttaacggatc
     9241 ttcagatgca cagcgtcatc tatcaactaa taacatgaac cttgtagacg tttttaggag
     9301 ctagccatgg ccgagcttac agttgtgggc aaaccagtta cgcgtgtcga tgccagtgaa
     9361 aaggtcacgg gtgaggcggt atatggatat gatctggtcc tgcccaatat ggtgtatggc
     9421 aaggtattat tcagtcccaa ggcacacgcc aagattaaga aaatcaacac cgagaaagct
     9481 aaagcctacc ctggtgttgt cgccgtggtc accggtgcgg atgcgccgtg gacgcatgga
     9541 gaaagtatca aggataagcc gttcttagct caggacaaag ttcggtatat cggtgaaccc
     9601 gtggccgctg tcgctgcaga agacgaggat accgcgcagg ccgcagtaaa actgatcgaa
     9661 gtggattacg aagacttacc ggtttacgta gatccggagc aggcgagcaa agccgacgcc
     9721 atcgcgattc acgaggattt ccccaattac cgtaaagctg agttcatcgt tccgggcgcg
     9781 catgccaaca tcgccgagca cttcaagctt cgcaccggtg atgtcggaga aggcttccaa
     9841 aattcagatc tcgtgttgga agaaaggtat tttgtaccga tgatccagca tgccgccatg
     9901 gaaccgcatt cggcgcatgc gcaatttgat aaacaaagcg gtcggctgac catctgggtc
     9961 gccaacgacg cgccgttccg tgcacttaac gaacttaccg aagcgttgga gatgccaaaa
    10021 gaaaaaattc gcctcattaa cccgcttcag ggcggcgggt tcggttccaa agggggcttg
    10081 aaggtggagc cgatcgccgt agcgctggcg ttccacacga acggaagacc ggtgcgggtg
    10141 aaatttaacc gcgaagaaac gtttatctca acgttgaccc ggcacgaagc aatcgtctac
    10201 tgcaagaccg gggtaaaaaa agacggcaca ctgatggctc gcgagatgac aatctactgg
    10261 ggcgctggtg cctatgctga aaagagcccg acggtctgca tccgcggcag tttgcccgcg
    10321 cccggcccat accggatccc gcacgtgaaa gttgatggct acgccgtcta tactaacaaa
    10381 ccggtggcag gctcgtatcg cggctacggc attccgcaag gcgcctgggc gggcgagcaa
    10441 cagatggatg aaattgccaa acgcctcggt atggatcccg ttgaaatccg catgaagaat
    10501 attttcgtcg aaggcgatat ctcttactgg ggcgagcagc tccacgccgt cggcttgaag
    10561 gaaactttga ccaaggcgat tgaagctatt gattggggta agaagccggc gcaaaccaag
    10621 ccgggtgtga aagtcggcaa ggggtttgcg tgtatccaaa agccgacgcg cagtccaacg
    10681 acatcggcgg caggcgtgat ggtcaacacc aaaggcgagg tcaccgttct cgcaggcacc
    10741 gtggagatcg gccagggctg taacaccatt ctttcacaag tagcggcgga agagctcaag
    10801 gtgccgatgg aaaaagttca catgcatcct ctcgacaccg atgtaattcc ctacgacgcc
    10861 tcgacgacct ccagtcggag cacgtatcat atgggcaatg ctgtgcgcgg cgcggcgatt
    10921 catgcgggtg aacagatcat ggaagccgcc agtcccatgc tcgaagccaa gacagatgat
    10981 ttagattgcc gagacggcaa ggtctttctc aaggatcaac cgcaaatggc tttgacgtta
    11041 ggcgaagtgg tgcgccgcaa actcggcgcc gaaggcgtcg ttcgcggcga ctcttcttac
    11101 acgtacgaaa tcggcaagga tctcgatctc gaaaccggtc acagcgatca cgcatcggct
    11161 ttttacatgt acgccacgca agctgcggaa gtagaagtag atgaggatag cggtcgggta
    11221 agaattctgc gaatgtccgc cgcccacgat
//
LOCUS       DASUTG010000134         8514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1503551, whole genome shotgun sequence.
ACCESSION   DASUTG010000134 DASUTG010000000
VERSION     DASUTG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503551"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF089_02965"
     CDS             complement(<1..492)
                     /locus_tag="VF089_02965"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX6172949.1"
                     /translation="MLEMWEEALRQLREKIGKQNFETWIKPIRIDSITGDGIVLGVPN
                     KFFRDWLSEHYFPQISEVLESLTHKELKISLAIDNQSNQSSAARAEKGPRADKKEERE
                     PNTKHPSRANNLVPKYTFENFVVGASNQFAHAASMAVANQPGDHYNPLFIYGGVGLGK
                     THLA"
     gene            857..2149
                     /gene="hemL"
                     /locus_tag="VF089_02970"
     CDS             857..2149
                     /gene="hemL"
                     /locus_tag="VF089_02970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6172950.1"
                     /translation="MITTKSKRLFESAKKKIPGAVNSPVRAWQAVTGVPRFIAHGEGS
                     RVVDVDGNEYIDYVGSWGPLILGHAHPVVTRALRDALRQGTTFGAPTEGELELADVVC
                     RWVPSIEKIRLVSSGTEATMSAIRLARAFTKRSKIVKFDGCYHGHSDGLLVKAGSGVA
                     TLGLPDSPGVVPALAAETLVAPYNNIAVVEKIFSKYGNKIAAVIVEPVCANIGVIPPA
                     PKFLPDLASITRRYGSLLIFDEVVTGFRLAPGGAQEVYRVRPDLTCLGKILGGGLPLA
                     AFGGRRDVMNLIAPAGPVYQAGTLSGNPLAVTAGLATLKRLSRRGTYQRLEKNGKYLA
                     EGFARVLARYEIKSTVNRAGSMMTLFFGVERVSNADEARHCDREKFARFFHGMLERGV
                     YLPPAPFEALMVSLSHSANDLHKTVAAFESWAKDDYRS"
     gene            2390..2752
                     /locus_tag="VF089_02975"
     CDS             2390..2752
                     /locus_tag="VF089_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172951.1"
                     /translation="MAGTATILRIELWQALLLFATAITLGQTKLIDLKALLIGGVFMG
                     VNFFLLAFGILWVLAPLAGKGKIRAGVSFLILKGIIFLGVLTVLFSRFQIDGLSFAVG
                     FSTLILAIFFEAITHSLK"
     gene            2762..3562
                     /gene="atpB"
                     /locus_tag="VF089_02980"
     CDS             2762..3562
                     /gene="atpB"
                     /locus_tag="VF089_02980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6172952.1"
                     /translation="MEHPFTWHALLSHDWQAILQQHTFFALMSALFVLLFAWRSRVAL
                     ARAQDPAIPAADLGVRNIAELLVGLVVSQSDAIIGKQGRKFVPYFGTFFLFILFSNLM
                     GLLPGFSPPTSNLNTTVALALVSFIGYNVIGLKEMGTGYLRHFVGPMTGLPARSFLGK
                     LAFVPLLLLSVAFFFVLEFASHLFRPVSLALRLFGNMMGDHIVIEVFMDLTKLVVPVI
                     FYALGTLVSIIQAAVFMLLSMIYVSLAISHGHDESHGHEQEHAQQSSH"
     gene            3629..3961
                     /locus_tag="VF089_02985"
     CDS             3629..3961
                     /locus_tag="VF089_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX6172953.1"
                     /translation="MRKVGSLIGAAFVLLMTSGLALAAAESAPATAGDSQVKVAVAIA
                     AGLAVAVAAFGAALGQGRVGATAMESIGRNPNAADRLFLPLLLSLALLEALALYGFVI
                     AFLLQGKI"
     gene            complement(4013..4468)
                     /locus_tag="VF089_02990"
     CDS             complement(4013..4468)
                     /locus_tag="VF089_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX6172954.1"
                     /translation="MSVRLSWEQYFMTITRQVAERSTCTRAKVGAVIVRDKSILATGY
                     NGAPAGMPHCTDVGCLIYESITPNGEIEQNCFRTIHAEMNAIAQAAKNGSTIKEASIY
                     ITHTPCIHCLKVLVNTGIKNIFYEKPYKLHTLEELLSYTQVDLHKIDLP"
     gene            complement(4493..5293)
                     /locus_tag="VF089_02995"
     CDS             complement(4493..5293)
                     /locus_tag="VF089_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX6172955.1"
                     /translation="MESRKPIITLTTDFGYADPFVGIMKGAILCITPNLQIVDLNHGI
                     APQDIRAAALTLAASVDYFPQGTIHVAVVDPGVGSERRPILIHSAGQYFVGPDNGVFS
                     FALKDKRSLLIVHLSNEAYFLQPKSRTFHGRDIFAPVAAHLARGVPVAEFGSPSRDWV
                     CLSWPAVERAGEFIVGEVLYIDRFGNLFTNIHERDLTGVVKEKIRVHLNDVEIQGLAQ
                     NYAAGDKKTYIALVNSWGLVEIASYNDSAARRSETRIGDKVRVELLTR"
     gene            complement(5462..6409)
                     /locus_tag="VF089_03000"
     CDS             complement(5462..6409)
                     /locus_tag="VF089_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172956.1"
                     /translation="MPKKLFKPLDFSKVKTYPAKKRFSKVQTAALGNVIRRGSRVGSF
                     IEGLPDILAAQSLKAIARKIAQTHTANRTVVLGMGAHPIKVGLSPLIIDFMERGIIDA
                     VALNGAGIIHDFELAFMGETSEDVAASLTDGSFGMAEETGAFINQAITEGSTQGLGIG
                     ASVGQAIVAARLKHQQLSILATAARIGIPVTAHIAIGTDIVHMHPKADGKALGYGSLR
                     DFRTLTCVVASLDNGVYLNFGSAVILPEVFLKAVSLARNLGHPVQKLTTLDLDFLAHY
                     RPMTNVVNRPTLGSGKGYHLTGHMEIMVPLLFAAILENL"
     gene            complement(6423..7001)
                     /locus_tag="VF089_03005"
     CDS             complement(6423..7001)
                     /locus_tag="VF089_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEX6172957.1"
                     /translation="MTKAIIEHFQESIRVKQGFLRDNLETLTQAIDVLVRAFQRGNKL
                     LLFGNGGSAADAQHIAAEFVNRFMIERPPLPAIAFTTDTSILTSIANDYSYNQVFAKQ
                     VQALGKKGDVAIALSTSGNSSNVIAAIEACKKLDITTIGLTGGTAGQMVGKVDFLLNV
                     SEGRNSARIQETHILVAHVICDLVDQRLFPSK"
     gene            complement(7349..>8514)
                     /locus_tag="VF089_03010"
     CDS             complement(7349..>8514)
                     /locus_tag="VF089_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6172958.1"
                     /translation="EFAKHLYPSLRKVLGDECPSAPFIEEYMRVTGLLHDLGHGPFGH
                     FFDDNFLHDFELTHEDLGQAIIQKYLADIIGQLRRSPSGPFAAGEELRPEDVAFPIKK
                     NGQEDYGKPKWVRMLQPLTSGIFTFDNLDYVSRDSYMCGIAGGPVDRARLMHYTFFSP
                     KGLTLHKAGNSALTMFLNTRLYLYTNVYYHRTTRAIDLHLRDIFPDTMRVIFPGNPLE
                     HMEEYLYLTDWSLLEEVSRWRRKKGEMEALAKEWETVLHREVKWKMAYDRTLSLNELH
                     YGMALIQVGDWEKRIRSALPPRLRSLRFRIDMATQDPRPENPFAMGKKQIHIFDPSTG
                     EITKESLAELFEFIPSKVVQFRVFALDHEHDQVLAEIAENTLNGQSASVRTSV"
BASE COUNT         1997 a         2101 c         2190 g         2226 t
ORIGIN      
        1 agcaagatga gttttaccca gcccaacccc gccatagatg aacaacggat tgtaatgatc
       61 gccaggctgg ttcgccactg ccatagacgc tgcatgagca aattgattgc tggcgccgac
      121 aacaaagttt tcgaatgtgt attttggaac taaattattg gctcgcgaag gatgcttggt
      181 attaggctcg cgttcttcct tcttatcggc tcgaggacct ttttccgctc gcgccgcgga
      241 agattgatta gattgattat cgatagcgag agaaatcttt agctctttat gagtcaaaga
      301 ttctagcact tcactaatct gaggaaagta atgttcgctc aaccaatccc tgaaaaattt
      361 atttgggacg ccaagaacaa taccatcgcc ggtgattgaa tcgatgcgaa tcggttttat
      421 ccatgtttcg aaattttgtt ttccgatttt ttctctcaat tgtctgagag cttcttccca
      481 catttcaagc atagttccgc accaataagt agcgaatttt aaaaagaaaa ttagtgtagt
      541 tacgaacaac gttatccaca gctgtggata atcaaaaaac agcttggctt tcagctattt
      601 accggaattg acaacgacct caatcgacaa aagtacccag cgagaaaagg tggcaatttt
      661 ttttgctagc tgttcgtcca caacgagcaa actcacgatg atgcttgagc gaagcgagag
      721 cagttacaaa aattgccgaa gcgcaagtca taatcctttt catagtccat ttcaaggaaa
      781 actttttcaa aaaaattttt cttatcgttt cagcattctt gacacactga cacgccggtg
      841 ctagctgact ccactgatga ttaccacaaa atcaaagcga ctttttgaaa gcgcgaaaaa
      901 aaaaattcca ggcgccgtga acagtcctgt gcgagcgtgg caggcggtca caggtgtgcc
      961 gcgttttatt gcgcacggtg aaggctcacg tgtcgttgat gtcgatggga atgagtacat
     1021 cgattacgta ggctcttggg ggccgctgat tctcggtcat gcgcacccag ttgttacgcg
     1081 tgcgctacgt gatgcgttgc ggcaaggtac gacattcgga gcaccaaccg agggcgaatt
     1141 ggaattggcc gacgttgttt gccgctgggt gccgagcata gaaaaaatac gtctcgtgag
     1201 ttctggcacg gaagcgacaa tgagtgcaat acgattagca cgagccttca ccaagcgcag
     1261 taaaatcgta aaattcgatg gctgctacca tggtcattct gatggactgc tggttaaggc
     1321 gggttcagga gttgccacgc tagggttacc cgatagtccg ggagtggtgc cggcgttggc
     1381 tgctgaaacg ctagtagctc cttacaataa tatagcggtt gttgagaaaa tcttttccaa
     1441 atacggcaat aaaatcgcgg cggtgatcgt tgaacccgtc tgtgccaata tcggcgtgat
     1501 tccaccggct ccaaagtttc ttccggatct ggcaagcatt actcgtcgat atggcagctt
     1561 gctcattttt gatgaagtgg tcacgggatt tcgtcttgca cccggtggtg ctcaggaagt
     1621 gtacagggtg cgtccggatc tgacctgcct gggcaagatt ctgggtggcg gcttgccgct
     1681 cgcggcgttc ggcggccgcc gcgatgtgat gaacttgatt gctcccgcag gtccggttta
     1741 tcaagccggg accttgtccg gtaatccgct cgcggtcacc gccggcctgg caacgttgaa
     1801 gcggctttcg aggcgtggca cttatcagag gctcgaaaaa aatggcaaat acctggcgga
     1861 aggtttcgcc agggtgctcg cgagatacga gatcaagtcg accgtcaacc gggctggttc
     1921 aatgatgacc ctgtttttcg gggttgaacg agtctccaat gccgatgaag ccaggcactg
     1981 tgatcgcgaa aaattcgcca ggttttttca cggcatgctc gagcgagggg tctacttacc
     2041 accagctcca tttgaagcgc tcatggtctc tctatctcac tccgctaacg atttacacaa
     2101 aactgtcgct gctttcgagt cgtgggctaa agacgactat cgtagttgat tacgctatac
     2161 cttcatgcta gaaaatttac cgcagtggta gccggacaaa aggaaccctg cccgggaggg
     2221 aagcagccgc ccggcttaac tttggtactg ccattctgaa ggatagctca tccccggatc
     2281 ggtaacattg cccatttccg ttgtcatgat gatgacgaat ctcattttac ccgtatcggg
     2341 tgagaggatg ttcgtgataa ggatctgttc aagctagcag aatgaaacca tggccggtac
     2401 agcgactatt cttcgcattg aactttggca ggcattgtta ctctttgcga ccgctataac
     2461 tttgggccag accaagctta ttgacctgaa ggcgcttctt attggcggcg tgttcatggg
     2521 ggtaaatttt tttttgctcg cgttcggcat cctctgggtc ttagcgccat tggcgggtaa
     2581 aggaaagatc agagcaggcg tctctttcct gattttgaaa ggtatcattt tcttaggtgt
     2641 attgaccgta ttgttttccc ggttccaaat tgacgggctg tcgtttgccg tgggattttc
     2701 cactctcatt ctcgcgatct tctttgaagc cataacgcat tcgctaaagt aaagataaac
     2761 tatggaacac ccctttacct ggcacgctct gttatcgcac gactggcagg cgatcctcca
     2821 acaacacacc ttttttgctc tgatgagtgc gttgttcgtt ttgctttttg cttggcgcag
     2881 tcgggtggct ctggcgcgcg cacaagatcc ggcgatccca gcagcggatt tgggagttcg
     2941 aaatattgcg gagttattag ttggcttggt tgtaagccaa agcgatgcaa taattggcaa
     3001 acaagggcgg aaattcgtgc cgtatttcgg tacgttcttt cttttcattc tctttagcaa
     3061 cttgatgggc cttctgcctg gcttctcacc gccgaccagc aacttaaata cgacggttgc
     3121 tttggcactg gtttcattca tcggctataa cgtgattggt ctcaaagaga tgggaaccgg
     3181 ttacttaagg catttcgtcg gtcctatgac tggtcttcca gcccggagct tcctcggtaa
     3241 attggcgttc gtgcccctcc tactgttgtc ggtggctttt ttctttgttc tggagtttgc
     3301 atctcatctt tttcgcccgg tgtctctcgc gctgcggctc ttcggcaaca tgatgggtga
     3361 tcacatcgtt attgaagtct ttatggacct gaccaagcta gttgtaccgg tgatattcta
     3421 cgctctcgga acactcgttt cgattattca ggcggcagtt ttcatgctct tgagcatgat
     3481 ctatgtcagc ttggcgatct cacatggcca cgatgaaagt catgggcatg agcaagagca
     3541 tgctcagcag tctagtcatt aagcgtaggg cgacggcgaa gaggcagagg agaagaggta
     3601 gagcccactc gctggaaagg aggcaaagat gaggaaagta gggtcgctta taggcgcagc
     3661 gtttgtctta ttgatgacat caggcctggc gctagcagcc gctgagagtg cgccagcaac
     3721 ggctggcgat tcgcaagtaa aagtggcggt tgcaattgct gccggtttgg cggttgcggt
     3781 cgctgcgttt ggtgccgctc tcggtcaggg gcgggtcggc gccacagcca tggaaagtat
     3841 cggtcgcaat cctaacgctg ccgataggct gtttctgccc ttgctgctaa gtctggcgct
     3901 gcttgaagcg ttggctttgt acggctttgt tatcgcattt cttctgcagg ggaaaatcta
     3961 actgccgcga gtaaattagc gagggcgtgg gaatacccgc gccctcgctt ttttacggca
     4021 gatctatttt gtgtaaatcg acctgagtat aactgagcaa ctcttccaac gtatgaagct
     4081 tgtaaggctt ctcgtaaaag atgttcttga tgcccgtatt aaccagcacc ttaaggcagt
     4141 gaatgcaggg cgtgtgtgtg atataaattg aagcctcctt gatggtcgat ccgtttttgg
     4201 cagcctgagc aatcgcattc atttctgcgt gaatagtacg aaaacagttc tgttcgattt
     4261 cgccgttggg tgttatggat tcatagatca ggcagccgac atccgtgcag tgaggcatcc
     4321 cggctggcgc gccgttgtaa ccagtagcta gaatactctt atcgcgcaca atgaccgcgc
     4381 cgacttttgc ccgagtgcaa gtggatcgct ctgccacttg ccgcgtgatg gtcataaaat
     4441 attgctccca actaagccgc acgctcatgg acgcctccgg atggcctgat tttcaacgag
     4501 taagtagttc aactcgcacc ttgtcgccga ttctggtctc actacggcgt gccgcgctat
     4561 cattataact cgcaatttcg actaatcccc agctattaac gagcgcgatg tatgtctttt
     4621 tatcgcccgc ggcatagttt tgcgctaggc cttggatttc cacatcgtta agatgcacgc
     4681 gaattttttc cttcaccacc cctgtcagat cgcgctcgtg aatatttgtg aagagattgc
     4741 caaagcgatc aatatagagt acttcgccga cgatgaattc tcccgctcgt tccactgccg
     4801 gccaagataa acatacccaa tctcttgagg gcgagccaaa ttcagctacc ggaactccac
     4861 gcgcaagatg agcggccaca ggggcaaaaa tatctctgcc gtgaaaggtt cggctcttgg
     4921 gctgcaaaaa ataggcttcg ttcgataaat gcacaatcag caatgatcgc ttgtctttaa
     4981 gtgcaaaact aaacacacca ttgtccggac cgacaaagta ctgtcccgct gaatgaataa
     5041 ggatcggccg ccgttcactg cctacgccgg gatcgacgac ggcgacatgg atcgtgccct
     5101 gcggaaaata gtcaactgac gcagctaagg ttaacgccgc cgccctgata tcctgcggcg
     5161 caattccgtg atttaaatcg acgatttgaa gattgggagt gatgcacaaa atcgctcctt
     5221 tcatgatccc gacaaacgga tccgcataac cgaaatccgt cgtcaacgtg atgatcggtt
     5281 ttctggactc cacggcaggg gtagtctaac cagaaaaacg agacagggta aagcataacc
     5341 gccacgcgca aataattgtg attcatctac tatttccgcg tcgtcttggc ggggcattaa
     5401 tctggcgcga atcatcttga ttgagacaat ttcagagaac atggagtaaa tatagcggaa
     5461 gctataggtt ttctaggatt gccgcgaaca gcaacggcac cataatctcc atgtgaccgg
     5521 tgagatgata acctttgccg cttcccaggg ttggacgatt gacgacatta gtcatagggc
     5581 gataatgagc gaggaaatcg aggtctagcg tcgtcaactt ctgtaccgga tgacccaaat
     5641 tgcgggccag gcttacggct ttaagaaaga cctcaggcag tattacggcg gagccaaaat
     5701 tcagatacac gccgttgtcg agggatgcga ctacgcacgt caaagtgcgg aagtctcgca
     5761 agctcccgta accaagagct ttcccatccg ccttcgggtg catatgaacg atgtccgtgc
     5821 ctatggcgat gtgtgctgtg actggaattc cgatgcgcgc ggcagtcgca agaatgctga
     5881 gttgctgatg ctttagacgc gctgcaacga ttgcttgtcc cacggaagcg ccgatgccga
     5941 gtccttgtgt ggacccttcg gtgatggcct ggttgataaa agcgccggtt tcctcagcca
     6001 tgccgaagct tccgtctgta agactggcgg cgacatcttc cgacgtctcg cccataaaag
     6061 cgagttcaaa gtcgtgaatt attccagcgc cgttgagtgc tactgcatca atgatacctc
     6121 tttccatgaa gtctataatg agcgggctta gtccgacctt aatcggatgc gcgcccatac
     6181 cgagcaccac cgtgcgattg gctgtgtgag tttgtgcgat tttacgagcg atagctttta
     6241 agctttgggc tgccaggata tcgggcaatc cttcaatgaa cgagcccacc cggctgcctc
     6301 ttcgtatgac gtttccgagc gccgcagttt gcaccttgct gaatcgcttc tttgccggat
     6361 aggttttaac cttggagaaa tcgagaggtt tgaaaagctt tttaggcatg gaacggctgg
     6421 gtttattttg aggggaagag tctctgatcc accagatcac aaatgacatg cgcgacgaga
     6481 atatgtgtct cctggatacg cgcactattt cgtccctctg aaacattgag cagaaagtct
     6541 actttcccca ccatttggcc tgcggtgccg ccagttaggc caatggtcgt gatatctaat
     6601 tttttgcaag cttcgatcgc ggcaatgacg ttagacgagt tgccgctggt cgatagcgct
     6661 atggccacgt cgcctttctt acccagggcc tgtacttgtt tcgcgaaaac ttggttgtag
     6721 ctgtaatcat tagcaatgct cgtcaatatc gacgtgtcgg ttgtgaaagc gattgccggc
     6781 agcggcggtc gttcgatcat gaatcgattt acgaactcag cggcaatatg ctgagcatcg
     6841 gcggcgctac cgccattacc gaagagaagt agcttattgc ctctttgaaa cgccctaacc
     6901 aaaacatcga tggcctgtgt caaagtttct aagttgtcgc gcaggaaccc ctgctttacc
     6961 cgtatgcttt cttgaaaatg ttcgataata gcttttgtca tgatgctact gggtcccatc
     7021 taaggcttgg gcttgcttta tctaacacac acccgtggaa gcgtaaagct caacgtgagc
     7081 ggtgtaaatt tggcaaatat gcttacttcg tttgtatccg cttccacccc caacaaattc
     7141 tagcgcgaga gtcgcccgaa agtagggcat ttatcgatct accccacaga agctctcctc
     7201 cgcagactaa agcagctttg aagatggcgc agagtggaac cgggattaga agcggctttg
     7261 ttagagatgc ctcgctcgct cgcgagacta ctcacccagg aagtcgattg gttggataac
     7321 tcgacaattg gaccgtcgag tatttttcct aaacactggt cctcacggag gcggattgcc
     7381 catttaaggt gttctctgca atttcagcga gcacttgatc gtgctcgtga tctaacgcaa
     7441 aaacacgaaa ttggacaacc ttggagggga tgaactcaaa aagttcggct agcgattctt
     7501 ttgtgatctc accggtggaa ggatcgaaga tatgaatctg tttcttaccc atggcaaaag
     7561 gattctccgg ccgcggatct tgggtcgcca tatctatgcg aaatctgaga ctgcgcaacc
     7621 ttgggggcaa agcgctgcga attcgcttct cccaatcgcc gacctgtatc agagccatcc
     7681 cgtaatgaag ctcattgagc gaaagtgttc gatcatacgc catcttccac ttgacttcgc
     7741 ggtgtagcac cgtttcccat tctttggcta atgcctccat ctcgcctttt ttccgccgcc
     7801 acctagagac ttcctcgagc agcgaccaat ctgtcaaata caggtactct tccatgtgtt
     7861 cgagcggatt acccggaaag atcacacgca tcgtgtccgg aaagatatcg cgcaagtgga
     7921 gatcgatggc gcgggtggtg cggtggtaat agacgttcgt gtacagataa agccgagtgt
     7981 tgaggaacat ggttagggcg ctgttgccgg ctttatgcag cgtcaaacct ttaggcgaga
     8041 agaaagtgta gtgcatcaga cgggcacgat ccaccggccc accggcgatg ccacacatat
     8101 aggagtctcg cgagacataa tcgagattat caaacgtgaa gatcccgctg gttagtggtt
     8161 gcaacattct cacccatttg ggcttaccat aatcttcttg accatttttt ttgatcggaa
     8221 aagcgacgtc ctctggccgc agctcttcac cggcagcaaa tgggccgcta gggctgcgcc
     8281 tcagttgccc aatgatgtcg gccagatact tttggatgat ggcctgcccc aaatcttcgt
     8341 gcgtcagctc aaagtcatgc aaaaagttat cgtcgaagaa gtgtccgaac ggcccatgtc
     8401 cgaggtcatg aagcaaacca gttacccgca tatactcttc gatgaatggt gccgacggac
     8461 actcatcgcc caggaccttt cgcaacgacg gatatagatg cttagcgaat tctc
//
LOCUS       DASUTG010000135         8626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1058793, whole genome shotgun sequence.
ACCESSION   DASUTG010000135 DASUTG010000000
VERSION     DASUTG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058793"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1363)
                     /locus_tag="VF089_03015"
     CDS             complement(197..1363)
                     /locus_tag="VF089_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6172959.1"
                     /translation="MARRVAGFGTTVFVEINDLARQCNAVNLGQGAPDFDGPPEVLAA
                     AVRAINGQLNQYAPGVGMQVLREAVARHAERFYGQNIDPHTEVLITSGATEAVFAAIL
                     GLTDVDDEVIVFEPVYDSYVPNMVMAGVRPRYVPLCGDQWSFDADELERAFNFRTRAI
                     IVNTPHNPTGKVYSRQELNVIADLCRKHQVVAITDEVYEHILYDAVAHTRLATLPGMA
                     ERTLTISSLGKTFSVTGWKIGWVIGPANLVNAVNRAHQFITFAVASPLQAAAAVALSL
                     PIKYFEELRLSYESKRNRMLKVLEQAGFRVLKPQGSYFIMADWRAVGPRHVRDDVQFA
                     RWLTTEVGVACIPPSAFYQESDKDLGRYLARFAVCKKDVTLAAAAERLGRLGKL"
     gene            1605..3104
                     /locus_tag="VF089_03020"
     CDS             1605..3104
                     /locus_tag="VF089_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6172960.1"
                     /translation="MAQSAASFNLIETTIDDIHAAYKSGRLNCRQLVRMYLDRIEAFD
                     KKGPQLNAIITINSEALSEADRLDAAYKVSGPVGPLHGIPIVFKDQGDVKGMPTTLGS
                     VLFKDFYPDRDSFVADKLKKAGAVILAKATLGELGGGDTHGSLFGSTRNPYDTERTVG
                     GSSGGSAAAVSANYSTLGVGQEGLASIRRPSTWNCIIGMRPTAGLVSRGGVYGCWPEV
                     NGSLGPMARTVGDLAALLDIMVGYDPEDPITARGIGHIPKTYKSFLDKNGLKGARIGI
                     LRESIGLGSEPDSQDFKKITEVFEKAVSELKASGAESIDPITIPKLKELMAKRSSGPG
                     DSDKSFQNYYGRSANPPFKSREEVIAAPEFAKVVRRSQERFKKNPDPMSHYEHLKAQD
                     ELMINYLKVMADHRLDAIVHKAIEHQPTLIKDGVNPPFVDQKGAPHLNTFLVFVPTIV
                     VPAGFTRDNLPAGVCFTGRAYDDGNLIKFAYAYEQATRHRRPPASTTGL"
     gene            complement(3273..3704)
                     /locus_tag="VF089_03025"
     CDS             complement(3273..3704)
                     /locus_tag="VF089_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX6172961.1"
                     /translation="MAETNLQKPLPTITSEARPFWDGAAKQKLLMQRCLACSAYIWTP
                     RPSCFECGSENIQWQELSGRGEVYSFTVIRQIVGRAASQAFEKDIPYVIAWIDLDEGP
                     RMITNVIGCPVDAVRVGMKVSVVFEQQSPEVWLPKFKPADL"
     gene            complement(3942..5135)
                     /locus_tag="VF089_03030"
     CDS             complement(3942..5135)
                     /locus_tag="VF089_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6172962.1"
                     /translation="MSIRGKAAIVGIGETPTDRLGSKPGETRRSSAQYLAWAMRLALE
                     DSGLTLKDLDGQGLGVTIPTAYPQPFWPEEVAEILGITPGFLLGGATGGAGAVSLLGG
                     MAAAINSGLIDTALCIGAAAPFSEHFGGGIQPADMRDFEIPFGTMGPNSKIAMVMRRH
                     MHQYGTTLDHLGKIAVAGRYHASLNPSAYLRKPITVQDYKDSRLVADPVRLLDCVLPA
                     NGGKAYIMASPERARNLRKPPVAVRGFGERSNPSYGPRAGSDALTMGVADAGRVAMEM
                     AGVKHGDINFLELYDDYIIVVYLQIEDLGFCAKGDLGFFERTDFTINGRLPIQTGGGM
                     INCGQPSTAGGTIHVIEAVRQLRGEAGERQVPGAKIGLVTGLGVLPYGKNLGCCAVAV
                     LGTEV"
     gene            5297..6286
                     /locus_tag="VF089_03035"
     CDS             5297..6286
                     /locus_tag="VF089_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172963.1"
                     /translation="MLKLAARMLGVVFAVTCFCSPADAKFVVAWSAVSALNSPFWVMQ
                     DAGFFKKEGLDVELVYIASSPTVARATLAGDIVISGANSQVIVDVGLNGGDLVAMGAI
                     TNVVAFYVMAAPEIKNVTDLKGKVVGVTRFGASTDFGMRMLLSKYGLEAVKDVPFIQI
                     GGMPELTAALSNRTVFAAPMSQPMVYLAQKAGMRMIANLAKEDIPFMHIGLTTSKKWI
                     RENRAQAKAFIRAYGRAVHFMHNHKEETRGIFARYTKINDPGMLDGSIQYGYDFMEKV
                     PLVKIEAFQVTLDQIARNNPKAKQVKPDQFFDNSLVQEVVDEGFFTKLWGKKP"
     gene            6464..7567
                     /locus_tag="VF089_03040"
     CDS             6464..7567
                     /locus_tag="VF089_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6172964.1"
                     /translation="MTLILNNDDVKQVLTMEVTMKALDQAYTELTRQEAVCRPRIDIQ
                     IPTNDPNKIYQWGTMEGGSMSGYFAIRMKSDVIYEQEYQGAITQEKYCVTPGRFCGLI
                     LLNNIQNGEPLALINDGYLQHMRVGADSGIGVKYMAREDAEVVGMIGSGGMARSHVES
                     FLLARKIKRIQVYSPTKAHREEYAKEVSEKYGIEVVPVSQPKEAYKSAHIVAGCTDSS
                     VPIVIGKWLEEGTHVTCVGGKPDDDTLKRIDISLRLGNAPHPWGLPEFGLADEYITYA
                     AKPDHNADFQMKRAGKRAHGVIAQDRAVLLEELLTGKKKGRTSDKQITYSERGNIQGA
                     QFFAVAGRVYELAKARGLGKEFPTEWLLQDIRD"
     gene            7638..>8626
                     /locus_tag="VF089_03045"
     CDS             7638..>8626
                     /locus_tag="VF089_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6172965.1"
                     /translation="MPLFLNNEEVEQLLTMKTCMDALEILYRELGEGTAVAASRTDIH
                     SPTAAAQGGEAPMAHYLKSMSGASPHFGTAALRFSSDIVAWRQAGEGMRREKIPALPG
                     NRWLGIVLLFSSANGELLAVMNDGVIQRMRVGGTNGVATRYLARADAQTVGLIGSGWQ
                     ATSQVVAVCEARVIKQIKVYSPTRANRERFATETAALVGVEIVPVNSQEEAVRQVDII
                     LTSTNARKPFLGPEALAEGVHLSCMQRDEANDEALARCRPLVVHTNLTENNSTSSQLK
                     KMAKPGFEFRDHPMQRGIDWKTLPTLPDLITGRIKGRENADQITGFVNNIGLGV"
BASE COUNT         2002 a         2321 c         2439 g         1864 t
ORIGIN      
        1 agcgcgcata acactgtggc gatgcggagc taaccttagc cggatctagg ttgatgcgtc
       61 aaccggaatt tccacctagt gcctggagtg gacacacgcc cttgaggagg tgagtgcccg
      121 gggtaaaacc ttttgatcac atgcgagata acagccgggc tgtacggctg ctcgcacccg
      181 cgtgagtgga gaccgctcac agttttccta gcctccctaa tcgttccgct gcggcggcaa
      241 gggttacatc tttcttgcat actgcaaaac gcgccagata tctgcccaga tctttgtcgg
      301 attcttggta gaaggcgctg ggcggaatac aggcgacgcc aacctcagta gtcagccagc
      361 gcgcgaactg aacgtcgtcc ctcacatgcc gaggcccgac ggcgcgccaa tccgccatta
      421 tgaaatagct gccctggggc ttgagaactc tgaaaccggc ttgttctaac accttgagca
      481 ttcgattacg cttggattcg tagcttaacc gcagctcttc gaaatacttg attggcaaac
      541 tgagcgccac tgcggccgcg gcttgcaatg gagatgcaac tgcgaaggtg atgaattggt
      601 gcgctcgatt caccgcattc accaagtttg ccggaccgat cacccagcca atcttccagc
      661 cggtaacgct aaaggtttta ccaagactgc taatggtcaa ggtacgttct gccatgcccg
      721 gcaatgtcgc cagtctcgta tgagcgactg cgtcgtagag gatatgctca tagacctcgt
      781 cggtgatggc gacgacctga tgcttgcggc agagatcggc aatcacgttt agttcctggc
      841 gagaataaac cttgccggtc ggattgtgcg gcgtgttgac gatgattgcg cgggtgcgaa
      901 agttgaaagc tctttcaagc tcgtcggcgt cgaaactcca ctggtcaccg cataaaggca
      961 catagcgcgg cctcacaccc gccatcacca tgttgggcac atagctgtca tagaccggtt
     1021 caaaaacgat cacttcgtcg tccacatcgg tcaagccgag gatcgcggcg aagaccgctt
     1081 cggtggcgcc ggaggtgatt agaacctccg tgtgcggatc gatattctgg ccataaaaac
     1141 gctcagcatg gcgtgccacc gcttcgcgca acacctgcat cccgacaccg ggggcatatt
     1201 ggttcaactg gccgttaatt gccctcaccg ccgcggccag aacttccggc ggaccatcga
     1261 agtccggtgc tccctggccg agattgactg cattgcattg tcgtgccaaa tcattgatct
     1321 cgacgaatac cgtggtgcca aatccggcga cgcgccgggc catagatgac atcgctgatg
     1381 aaccccctcg cagagattct tcgggataat cattaaccgc gtggatttca acggtgctga
     1441 gatcgagtcc ctgttgcgcg tgtaagtggc tgcggcggac ggagaaagtc tatacgcagg
     1501 ggtgtcgggg acatcaactc ggcgattaaa aactcctgga catttttacc gtatttaaat
     1561 atcagaagtt aagcaaggga agcgaaatca aaggagttca tgccatggct caatccgctg
     1621 cgtcctttaa tctcattgaa acgaccattg acgatattca tgcggcttac aagtcaggtc
     1681 ggctaaattg ccggcaacta gttcggatgt atctcgaccg aatcgaggct ttcgataaaa
     1741 aggggccgca actcaatgcg atcatcacga tcaattccga ggcgctgtct gaagccgatc
     1801 gtctcgatgc ggcgtacaaa gtttcgggtc cggtgggacc actgcacggc atcccgatcg
     1861 tcttcaagga tcaaggcgat gtcaaaggca tgccgaccac cttgggatcg gtcttgttca
     1921 aggatttcta tccggatcgg gattcgtttg ttgctgacaa gctaaaaaag gcaggcgccg
     1981 tgatccttgc caaagcaaca ttgggcgaac tgggtggcgg cgacacacat ggctcgctgt
     2041 tcggttcgac ccgcaatcct tatgatacag aacgcaccgt cggcggttcc tccggtggtt
     2101 ctgcggcggc ggtctcggca aactattcga ccctgggcgt cgggcaggaa gggctggcct
     2161 cgatccgcag gccatcgacg tggaactgca ttatcggcat gcgtccgacg gcaggcctgg
     2221 tcagccgcgg cggagtttac ggttgctggc cggaggtcaa cggatcgctc ggtcccatgg
     2281 cccgcaccgt cggcgactta gctgctttgc tggacatcat ggtggggtat gatccggagg
     2341 atccgatcac cgcgcgtggg atcggccata ttcccaaaac ctacaaaagt tttctcgaca
     2401 aaaatggtct taaaggagcg cggatcggca tcttgcgtga atcgataggc cttggcagcg
     2461 aaccggattc acaagacttc aagaagatca ccgaggtgtt cgagaaggca gtgagcgagc
     2521 tcaaggcgag cggcgccgag agtatagatc cgatcacgat tcccaagctc aaggaattga
     2581 tggcgaagcg atcttcaggt cccggcgact cggataagtc ttttcagaat tactatgggc
     2641 ggagtgctaa cccgccgttt aaatcccgtg aagaggtcat cgccgcgccg gaattcgcca
     2701 aggtcgtgcg ccgttcgcaa gaacgtttca agaagaatcc cgatcccatg agccattatg
     2761 aacatctcaa agcgcaagat gagttgatga tcaattacct gaaggttatg gccgatcatc
     2821 gcctggatgc gattgttcat aaagcgatag agcaccagcc aactctgatc aaagacggcg
     2881 tgaacccgcc gttcgtcgat cagaaagggg cgccgcatct gaacacgttt ctagttttcg
     2941 ttccgacgat tgtcgtgccg gcgggattca cacgtgataa tctccccgcc ggtgtttgct
     3001 ttaccggacg agcgtatgat gacggaaact tgatcaaatt tgcgtacgcg tacgaacagg
     3061 ctacgcgtca tcgccgcccg ccggcaagca caacgggact gtgagataaa cgtttcaaac
     3121 gttccagcag ttccaatcgt tccagcggtt caaaacggga aggcgtgcga taatttgctg
     3181 agcagaggcg tacagcggca gtacattaat gcggggcggt tcagccggaa cgaaaaacgc
     3241 aaagttcttt cagcaggcag ccagcccgac tattataaat cggcgggctt gaacttcggc
     3301 agccagactt ccggcgattg ttgttcgaac acgaccgaca ctttcatgcc gactctgact
     3361 gcgtcaaccg gacagccgat cacgtttgtg atcatccgtg ggccttcatc caggtcgatc
     3421 caggcgatca cgtaaggaat gtctttttca aacgcctgcg atgccgcgcg accaacgatc
     3481 tgccggatca cggtgaagga ataaacctct ccccgaccac tgagctcctg ccattgaatg
     3541 ttttcgctgc cgcattcgaa acagctcgga cgcggtgtcc agatataagc gctgcaagcg
     3601 aggcagcgct gcattagaag cttctgcttc gctgctccgt cccaaaaagg ccttgcttcg
     3661 ctggtgatcg taggtaacgg tttttgcaga ttggtttcag ccatgggctc cttgaggtta
     3721 gttcacgttc aaccgttcaa aatttcaacg tcagaaatta ttttttcaat gggactgttc
     3781 aatgttgtta ccaagctgct ctcgcttcgt catttcgacg gtgaaatctt cctactgaaa
     3841 gatgctccaa cgttcgtcgc atcgatcgca agacaacaag agtcttgcga tcttgtgatc
     3901 ttactgcagt tggatctgca actgcctcgc gcgcctttta gtcacacttc tgtacccaag
     3961 accgccacgg cacagcagcc aagattcttt ccatacggca ggactcccaa gccggtaacc
     4021 aggcctattt tggcgccggg cacttggcgt tcgccggctt cgccgcgtaa ctgacggacg
     4081 gcctcgatta cgtgaatggt tccacccgca gtagaaggct ggccgcagtt gatcatgccg
     4141 ccgccggttt gaatcggtag ccggccgttg atcgtgaaat cggttcgctc aaagaagccg
     4201 agatcaccct tggcgcaaaa ccctaaatct tcgatctgca agtaaaccac gatgatgtaa
     4261 tcgtcgtaga gctcgaggaa attgatatcg ccgtgtttca cgcccgccat ttccatggcg
     4321 actctgccgg catcggcgac acccatggtc aacgcgtcag aaccggctct cgggccgtat
     4381 gacggattgc ttctttcacc aaaaccgcgg acggcgactg gcggcttgcg caggtttctc
     4441 gctcgttcgg gcgacgccat gatgtaggct ttgccgccgt ttgccggcag cacgcagtcg
     4501 agaagccgaa ccggatcggc caccaatctg gagtctttat aatcttgaac cgtaatcggt
     4561 tttctcaagt aggcgctggg attcaaactc gcatggtaac ggccggcgac ggcgattttg
     4621 cccaggtgat ccaaagtcgt gccgtattgg tgcatgtggc ggcgcatgac catggcgatt
     4681 ttgctgttcg ggcccatggt gccaaaggga atttcgaaat cgcgcatgtc ggccggttga
     4741 ataccgccgc caaaatgttc agagaacggt gcagccgcgc cgatgcacag agcggtatca
     4801 atcaaacctg agttgatcgc tgccgccatg ccgcctagga gtgacaccgc tccggcgccg
     4861 ccggtggcgc cgccgagcag aaagcccgga gtgatgccga gaatctcggc gacctcttcg
     4921 ggccaaaacg gctgtggata ggccgtgggg atcgttaccc cgagaccttg tccgtcgaga
     4981 tctttgagag tgagccccga gtcttccaac gccagacgca tcgcccaggc caaatactgt
     5041 gcgctcgatc tgcgtgtttc gcctggttta gaaccgagac ggtccgtggg cgtctcgccg
     5101 attccgacaa tggccgcttt gccgcgaata ctcatagtcg gattgttata gtgtggctca
     5161 cgagagaaat gcaaacttcg aagtgtgtcc tcgctcagat ttcgttcgtc gcttgacaca
     5221 aagcaacgct gttctgtaca gcatgggtaa ccggtgctgg attccaagct gtgtgagcca
     5281 atgcaagggg gtctgtatgt tgaaactcgc ggcaagaatg ctcggcgttg ttttcgcggt
     5341 gacctgtttc tgcagccccg ctgatgcgaa attcgtcgtt gcctggtccg ccgtcagcgc
     5401 gttgaactcg ccgttctggg taatgcagga tgccggcttc tttaaaaagg aaggattgga
     5461 cgtcgagctg gtttacatcg ccagctcgcc gaccgtggcg cgggcgactc ttgccggcga
     5521 catcgtcata tcaggcgcca atagccaagt gatcgtcgat gtcggactca acggcggaga
     5581 tcttgtcgcc atgggcgcga tcaccaatgt cgttgcattt tacgtgatgg ccgcgcccga
     5641 gatcaaaaat gtaaccgacc tcaagggcaa ggttgtcggc gtgacccgct tcggcgcttc
     5701 aaccgacttc ggcatgcgca tgctcctgtc caagtatggt ctcgaagccg tcaaagatgt
     5761 tcctttcatt caaatcggcg gtatgcccga gcttacggcg gcgctctcca accggacggt
     5821 tttcgcagcg ccgatgtcgc aaccgatggt ttatctagct cagaaagcgg gaatgcgcat
     5881 gatcgccaat ttagccaagg aagacattcc gttcatgcac atcggtctca ccacatcgaa
     5941 aaaatggatc agagaaaatc gcgcccaagc caaggcgttc atccgcgcct atgggcgggc
     6001 cgttcatttc atgcacaacc acaaagaaga aacccgcggc atttttgcgc gctataccaa
     6061 aatcaacgat ccgggaatgc tcgatggcag tattcagtac ggttatgatt tcatggaaaa
     6121 agttcctctc gtcaaaatcg aggcgttcca ggtgactctg gatcaaatcg ccaggaataa
     6181 tcctaaagcg aagcaagtta aaccggatca attcttcgac aacagtttgg ttcaggaagt
     6241 tgtcgacgag gggttcttta ctaaattgtg gggaaagaag ccgtgaaaat ttctagtcgg
     6301 aggccgagcg ctaaacctgc ggtttatcga ttcgcgccgc aaagacgcaa aggcgcaaac
     6361 gtaagaacca agttggcgaa ctggcgggag tgatcgtctt gtctcagtga aaaaatgccc
     6421 aagcccttga aaacctcaag ctagttaaca caaaggaaga tctatgaccc ttattctcaa
     6481 caacgacgat gtgaaacaag tgctgaccat ggaagtcacc atgaaggcgc tggatcaggc
     6541 ttatacagag ttgacccgcc aggaggccgt gtgccgacct cgcatcgaca ttcagatccc
     6601 gactaacgat cccaataaga tctatcaatg gggcacgatg gaaggcggat cgatgtccgg
     6661 ttatttcgcg attcgcatga agtcggatgt tatctacgag caggaatacc agggcgcgat
     6721 tacccaggag aaatactgcg tgacgccggg aagattctgc ggcttgatct tgcttaacaa
     6781 tattcaaaac ggcgaaccgc tggcgctgat caatgatggt tatctacaac atatgcgtgt
     6841 tggcgccgac tctggtatcg gcgttaagta catggcgcgt gaagacgccg aagtcgtcgg
     6901 catgatcggc tccggcggga tggcgcgttc gcatgtcgaa tcgtttctcc tggcccgaaa
     6961 aatcaaacga atacaggtct acagtccgac caaagcgcat cgggaggaat acgccaaaga
     7021 ggtcagcgaa aaatatggaa tcgaggtagt tcccgtgagc cagccgaaag aagcctataa
     7081 aagcgcgcat atcgtcgcgg gctgcacgga ttcatcagtg ccgatcgtca tcggtaaatg
     7141 gctcgaagag ggaacgcatg ttacctgtgt cggtggcaag cccgatgacg ataccttgaa
     7201 acgcatcgat atctcactgc ggctcggcaa tgcgccgcat ccgtgggggc tgccggagtt
     7261 cggcctcgcc gacgaataca taacctacgc cgcgaaaccc gatcacaatg ccgattttca
     7321 gatgaagcgg gcgggcaaaa gggcacacgg ggtcattgcg caggaccgcg ctgttctcct
     7381 ggaggaactt ctaaccggaa agaaaaaagg gcggacatcg gataagcaga ttacctactc
     7441 tgaaaggggt aatatacagg gcgctcagtt ctttgcggtg gccggtaggg tttatgagct
     7501 cgcaaaagcg cgaggcctgg gtaaagagtt tccgaccgag tggctgctgc aggatatcag
     7561 ggactagcag agatgcctgt cttcgtcagg ctcacacggt tcggattaaa tcatctcagc
     7621 acgagcacga ggaagctatg ccgctatttt tgaacaatga agaagttgag cagctcctga
     7681 cgatgaaaac ctgcatggat gcgctggaga tcctttatcg ggagctcggc gaaggcacag
     7741 ccgtggcggc ttcacgcacc gatatccatt caccgacagc ggcagcgcaa ggcggcgagg
     7801 cgcccatggc gcattatctc aagagcatga gcggcgcatc gccgcatttc ggcaccgcag
     7861 ccttgagatt ttcttcggat atcgtcgcct ggcgccaagc gggcgagggc atgcgccggg
     7921 aaaaaatacc ggctctgccg ggtaatcgct ggctgggtat tgtattgctt ttcagctcgg
     7981 ccaatggcga acttttggcg gtgatgaacg acggcgtcat tcaacgcatg cgggtcggcg
     8041 gcaccaacgg tgtggctacc agatacttgg cgcgggcgga tgcgcaaaca gtcggactga
     8101 tcggctccgg ctggcaggct acatcgcagg tggtggccgt ttgcgaagcg cgcgtgatca
     8161 agcagatcaa ggtttatagc ccgacccgag ccaatcgaga gaggttcgct actgaaacgg
     8221 cggcgctggt cggtgtggag atcgtgccgg tgaattcaca ggaagaggcg gttcgccaag
     8281 tcgatatcat tctcacctcg accaatgcgc gcaaaccgtt tctcggaccc gaggcgcttg
     8341 ccgaaggcgt gcatcttagc tgcatgcaac gcgatgaagc gaacgatgag gcgctggccc
     8401 gctgccggcc gctggtggtt catacgaatc tgacggaaaa caacagcacc tcgtcgcaac
     8461 tgaagaagat ggcaaagcca gggtttgagt tccgcgatca tccgatgcaa agaggcatcg
     8521 attggaaaac cctgccgacc ctgcccgatc tgattacagg gcgcatcaaa ggacgggaga
     8581 acgcggatca gattaccgga ttcgtcaata acatcggtct cggtgt
//
LOCUS       DASUTG010000136         8092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_317874, whole genome shotgun sequence.
ACCESSION   DASUTG010000136 DASUTG010000000
VERSION     DASUTG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317874"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VF089_03050"
     CDS             complement(<1..800)
                     /locus_tag="VF089_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6172966.1"
                     /translation="MILSACAASVSPFPSPALAAADSASTKDRYIKWLGASSWILSSG
                     NDLVVVDPFFTRPSFVRVAASLMTPYLTGNFTYDPERIRAVLPELPKDTKFVLLSHAH
                     YDHLLDVPYYMNHNSGRNLTYVGSLTARNILLGFKPPALDFAIAEQNPSVERGGVRVT
                     ALPSDHAPHFFGHTFMAGDVVSPMTEIPSRVGQYLEGQTLVFLVDFLDEAGDAVWRVF
                     INGAASSAAGAEALRKHQSLLNERRINVAILCVPGWDKVDDYPDSLLRL"
     gene            complement(927..1766)
                     /locus_tag="VF089_03055"
     CDS             complement(927..1766)
                     /locus_tag="VF089_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6172967.1"
                     /translation="MRSTELDDPMVPVPIPEQATAKDGMAELPGTHLGYWDTGGAGEP
                     VVFLHPASGSALIWLYQQPVFSSAGYRTIAYSRRNYHNSDPALDDNPGTGSEDLHNFI
                     EFLGLKNFHAVSSAAGGSVAADYALSHPERLLSLTVSSNNLAAANGYIAEAAARIKLP
                     EWDALPRWFRELGPSYRAANPGGVAKWIELNLQSELGMGARQKLYNVVTPEKLQTLSV
                     PTLLMTGAADMFTPPAITRMIARHVPDNEVVIAAECGHSIYWERPHFFNRTVLGFIGR
                     HSN"
     gene            2124..2663
                     /locus_tag="VF089_03060"
     CDS             2124..2663
                     /locus_tag="VF089_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172968.1"
                     /translation="MNPMFIVASPIVLVIIGMVLNSTGLSFDQPPTSSEQDPAKRSAA
                     ELDSYRRFFDLQRKQAVKRQARVGQYGWLLMAAIIGSFIWLYMYTVDKTSLATQIATV
                     QTLPTEEGKETVLSITLKDGSNVKYLIKPPTAEMSEASKKDGMSKEKVSSYEMSQLGT
                     AFNVGESPLAPGVTLKVSQ"
     gene            complement(3308..3769)
                     /locus_tag="VF089_03065"
     CDS             complement(3308..3769)
                     /locus_tag="VF089_03065"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6172969.1"
                     /translation="MRLLELKVPPLAIGVLVAALMWLVSWAVPALNFVIQGRTLLALI
                     FALAGAIIIVFGVASFRRAKTTINPMKPESSSSLVLSGIYKFSRNPMYLGFLLALFGW
                     AVFLSNALSFVCLPAFIYYMNRFQIEPEEKALAGKFGEEYVAYKSRVRRWL"
     gene            complement(3779..4489)
                     /locus_tag="VF089_03070"
     CDS             complement(3779..4489)
                     /locus_tag="VF089_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6172970.1"
                     /translation="MSDRPTALVIDDEKQIRKLLRIVLEEDHYRVLESDSGKQGLADV
                     AVRRPDVALLDLGLPDMEGITVLKRLREWSQVPVLILSVRDGPEDKVAALDAGADDYV
                     TKPFEALELLARLRAIQRRSHSAADEPFFHAGHLAIDFTSHAVTLKGHEVKLTPTEYA
                     LLKILAQHAGKIVSHKQLLREVWGPNAEEQSQYLRVYMTHLRKKIDSSERGEKLLRTE
                     SGIGYRLVTPAAGNQQEA"
     gene            complement(4486..5988)
                     /locus_tag="VF089_03075"
     CDS             complement(4486..5988)
                     /locus_tag="VF089_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6172971.1"
                     /translation="MASTVNEYFLAGLLILTFTVVAWLIEPFTGYQAVALLYLLLVVL
                     LGLKLSRGPVLTVAVTSAVLWNFLFVPPYFTIYVQKFHDGIMFVVFIAVALAMGHLTS
                     RVRRSEITERQRERRTAALYELAQQAAFATDLNTGLGAAVNLIDSIFAANTALLVRQK
                     DHTLSKLPHAVSSFALSDAEKSVAGWVFSRHMPAGKFTESFSEAEALHIPLQGRTAVM
                     GVLSVRPPAGKPFDPSEMDLLEAFAVLIGLVLEKNHIVEAFKRAEILEASEHLRHALL
                     QSVSHELKTPLSAIQTGIGAIARQIEGDEKKQRTLREVQLAVRRLHRVINNLLDMTRI
                     ESGVIQPKLDWCDVGELIQAAIELAGESVAEHQITIDFDENVPMVRVDQPLLEQCLCN
                     LLLNSAANSAPGTKITIGAQLSEGQLIISVVDEGKGIRRDDLLRIFDAFYRGGDAAPG
                     GTGLGLAIVDGFVRAHGGTVRAANRHTGGAEFVIKVPVEILRPDIMENLA"
     gene            complement(6182..>8092)
                     /locus_tag="VF089_03080"
     CDS             complement(6182..>8092)
                     /locus_tag="VF089_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6172972.1"
                     /translation="VAFLAWVGLGADGLSSSAYGPEEAYLALGEHFFLALPLALLMAL
                     TVFVISASYSHIIELFPTGGGGYLVATKLLGPKVGLVSGSALIVDYMLTITISVASAG
                     DQIFSFLPISTHGFKLALEFSLIFFLIYLNLRGTKESVLFLLPIFLLFVVAHIFAIGL
                     GIIPKAAEVPLIASATYQKTIGDIQGLGLWATLFIVLHAYSLGGGTYTGIEAVSNGLQ
                     ALREPRVETGKKTMLYMATSLAFTASGLLLCYLLNQVSHETGKTLNASLFAKVYGGFF
                     SPELAYLLVIITLITEAAILIVAAQAGYIDGPRVLSSMAIDSWVPHRFAHLSDHLVTR
                     YGIWFMGLASLVFLLYTGGDVRLLVVMYAINVFLTFSISQLGMCRHWWEVRHTEKLWL
                     RRLFVNGLGLSFTATILIVTVTLKFAEGGWITVLVTVSFIVLCYGVRLHYDRVGSALK
                     RLDDTLMNIPFQPDLKDPVPAKNADAPTAVLIVRDFDGLAIHSLLSIGRLFPNHFKNL
                     VFVSIGVIDSGRFKGFSEIESLRKMKEDDLQSFVDFANCLGWYAEYRYALGIDLMEEL
                     EKLCKSVAKEFPRSVFFAGKLVFQEENFLTRLLHNHTPFTLEQRLQFEGLEMMILPIR
                     VFISGSQQPSTA"
BASE COUNT         2026 a         2150 c         2152 g         1764 t
ORIGIN      
        1 agtcgaagaa gactgtccgg ataatcatct actttgtccc atcccggaac gcacaagatc
       61 gcaacgttga tccgacgctc attgaggaga ctctgatgtt tgcgcaacgc ttcagcgcct
      121 gcggcactgc tagcagcacc gttgataaaa acacgccaaa ctgcgtcgcc ggcctcatct
      181 aagaagtcga ccaaaaaaac caaggtttgg ccttccagat attggccaac ccgggaagga
      241 atctctgtca tggggctaac tacatcacca gccatgaacg tatgaccaaa gaaatgcggg
      301 gcatggtcgc tgggtaaagc agtcactcta accccgcctc tttcaaccga ggggttttgt
      361 tcggctatcg caaaatcaag agcgggtggc ttgaagccca ataggatatt tcgcgccgta
      421 agactgccga cataggtgag atttcttccg gagttatggt tcatgtagta cggcacgtcg
      481 agtaaatgat cgtagtgcgc atggctcaag agaacgaact tggtgtcttt gggtagctcc
      541 ggaagtaccg cccttattct ttccggatca taggtgaagt tacctgtgag atagggcgtc
      601 atcagagatg cagccacccg cacgaacgaa ggacgcgtga agaaagggtc cacgaccacc
      661 agatcattgc cgctggagag aatccacgaa gatgcgccga gccacttaat atagcggtcc
      721 ttcgttgacg ctgagtcagc agcagccaga gcgggcgagg gaaaaggcga aaccgaagca
      781 gcgcaagcgc tcaggatcat cgtgcacgcc gtaagccaag caagcagggg agtcaattta
      841 tttcgcttga gcatgccgcg ggtctgacat cattcaaagt tggaggcgtg agctcgatcc
      901 catcggcggt gttccaggtc gagtttctaa ttcgagtggc ggccaataaa tccgagcacg
      961 gtgcgattga agaaatgggg tcgctcccag tagatcgagt gaccgcattc agccgcgatc
     1021 acgacctcgt tgtccggaac atgccgcgct atcatccggg tgatcgcagg cggcgtgaac
     1081 atgtcggcgg cgccggtcat cagcagtgtc ggcacgctca atgtctgcaa cttctccggc
     1141 gtgacgacat tgtaaagctt ttggcgggcg cccatgccga gctcggactg aagattaagc
     1201 tcgatccatt tggcaacgcc tccaggattt gccgcgcgat aggagggccc gagctcgcga
     1261 aaccagcgcg gcagcgcatc ccactctggc agtttgatcc tggccgcggc ttcggcgatg
     1321 taaccgttgg cggcagccaa gttgttgctc gataccgtaa ggctcagcag ccgttccgga
     1381 tgcgacaagg cataatccgc ggcgacactg ccgcccgcag ccgagctcac ggcgtggaaa
     1441 ttttttaggc cgaggaattc gatgaagtta tgcaggtctt ccgatcctgt ccctggattg
     1501 tcgtcaagcg ccggatcgga gttatgatag ttgcggcgcg aataagcgat cgtccgatag
     1561 ccggcgctgg agaataccgg ctgctgatat aaccagatca gcgcgcttcc cgacgctgga
     1621 tgcagaaata cgaccggctc tccggcgccg cccgtgtccc aatagccaag atgggtgccg
     1681 ggaagctctg ccatcccatc ctttgctgtc gcctgttccg gtatcggcac gggaaccatc
     1741 ggatcgtcca actcagtgga ccgcatgctc tcgttcatca tggttgttac gtattctata
     1801 gcatcaggta gaattccgcg tccaaatttt ggaccagcag cggatccgcg tcttcacact
     1861 ctattttgtg aaatatatca tctcctcggt gacacttatc aatgagcagt ttggagtcta
     1921 gtgttccctg gatagagtca ccacatcgtg gctcgagttt aaggcatacg acagccggat
     1981 cgattgtttt taatgtttaa gagctgcttg gtcgatcagc aatcaatcgt catgagtccg
     2041 atcgaaatag cccttgagct cgatggctgg gggcgttaag cttgcctggc cttatctcgg
     2101 ccgtatccgc aaaggagaga gttatgaatc ctatgttcat agtcgcaagt cccatagttc
     2161 tagtgattat tggcatggtg ctcaatagca ctgggctttc attcgatcag ccgccaacct
     2221 catcggaaca agatcctgca aagagaagcg ctgcggaact cgattcatac cgccgtttct
     2281 tcgatctaca gagaaagcaa gctgtgaaaa ggcaggcacg agtcggccaa tatggctggc
     2341 tgctgatggc ggcgattatc ggttccttta tttggctgta catgtacacc gtagataaga
     2401 ccagcctagc cacacagatc gcaacggttc aaaccttacc gaccgaagaa ggcaaagaga
     2461 cggttctctc gataactctg aaagacggga gcaatgtcaa atatctaatc aaaccgccca
     2521 cagccgagat gtcggaggcc agcaagaaag acggaatgtc caaggagaaa gtctcatcct
     2581 acgagatgtc gcaattaggc accgctttta atgtgggcga aagtccatta gcacccggcg
     2641 tgacgctgaa ggtttcccaa tgacgagcag caaggcggct gcagttacgg gctcacgtaa
     2701 acggaatcaa gtcccccgcg cgaacactcg gccgcagcgc gaggctggat gctgtggcgc
     2761 accatgtaaa ttcggtaata ctggattgtc gttcattgcc gatccgaacg gtagcccggg
     2821 tggtgctcgg atcgttgcag agtattgtac ttattcctct ctcgactaga tttcttggcg
     2881 cctgcttgcg tcaccagccg tttgacatga atggtccgct gtaaagtcat cgcttatcgc
     2941 ctcgatggct gttgccaaca gggtttagct gattgcccgt caccgctcgg tagcttacaa
     3001 gtcgactgga ccgatgccca cgagatcgac ggtgtgcgcc agcctgcttc gattcgatgg
     3061 atgaagccga gaaaggtgcc gatcatccgg caaggagttt caggagattg cattggatcc
     3121 cgatggatcc cgtgcaacgg gagaaggttt accgccgctc gggcgagaaa gcataaacaa
     3181 ttacgttaaa gcgaagggga gcttgcctga ttcaagcgcg cacgaaaggt aattcctaat
     3241 ttcacgccca tctatgatgt gtttcagtaa ctagcgcaat tttcaggagt gagaagcttg
     3301 taacctctca gagccaccga cgtacccgag atttataggc aacgtattct tcgccgaact
     3361 tcccagccag cgctttttcc tcaggttcga tttggaaccg gttcatgtaa tagataaacg
     3421 caggcaagca aacaaaagat agagcatttg ataagaacac tgcccagccg aacagggcta
     3481 aaagaaagcc tagatacatt ggattgcggc tgaacttgta tatgcccgaa agcaccaagg
     3541 acgatgacga ttccggcttc atcgggttga ttgtcgtctt tgctcgtctg aaggaagcaa
     3601 caccgaagac gataatgatt gcgccggcca aagcaaaaat cagcgccaac aacgtgcgtc
     3661 cctggattac aaaattaagc gccggcacag cccacgagac aagccacatg agcgcagcca
     3721 ccaaaacgcc gatagccaga ggcggcacct tcaattcgag aagacgcata gcgactgctc
     3781 aagcttcctg ctggtttccc gctgctgggg tgaccaaacg gtagccgatt ccactctcgg
     3841 ttcgtagcag cttttcgcct cgctctgagc tgtcgatttt cttgcgcagg tgcgtcatat
     3901 agacccgcag atactgcgac tgctcttccg cattcggacc ccaaacttca cgcagtaatt
     3961 gcttgtgcga aacgattttg ccggcgtgct gcgcaagaat tttaagcagc gcatactcgg
     4021 tgggcgtgag cttgacttcg tgtcccttca gagtgactgc atgactggta aaatcgatgg
     4081 cgagatgtcc cgcgtgaaaa aaaggttcgt cggccgctga atgagaacgc cgctggatgg
     4141 ctcgtagacg cgccaggagt tccagcgctt cgaagggctt ggtgacgtaa tcgtcggctc
     4201 cggcatctag ggcagcaacc ttatcttccg gaccgtcacg gacggataga attaaaaccg
     4261 gcacctggct ccactcgcgc agccgtttca gaaccgttat gccttccata tcgggaagtc
     4321 caagatcgag taacgcaaca tccggtcttc ggacggcgac atcggcgagc ccctgtttgc
     4381 cgctatccga ttcaagcacc cgataatgat cttcctccaa cacaatgcga agaagctttc
     4441 ggatctgctt ctcatcatcg attaccaagg ctgttggtcg atcactcatg ccaagttctc
     4501 catgatatct ggccgaagga tttccaccgg aaccttaatg acgaattcag ctccgccggt
     4561 gtgtcggtta gctgcgcgca ccgtgccgcc gtgtgcgcgg acaaaaccat cgacaattgc
     4621 gagaccgagc cccgtgccac caggagctgc gtcaccgccg cgatagaagg catcgaagat
     4681 gcgcagtaga tcgtcgcgcc gaattccttt tccctcgtct actacggaaa taatcagttg
     4741 cccctctgac agctgagcgc cgatcgtgat cttcgtccct ggcgcgctgt ttgcggcaga
     4801 gttcagaagc agattacaca agcactgctc cagaagcggc tggtcaactc tgaccatcgg
     4861 gacgttctca tcaaagtcga ttgtgatttg atgctcagca acggattcac ctgcgagttc
     4921 gatggctgct tgaatgagtt cccccacatc acaccagtcg agcttcggct ggatcactcc
     4981 ggattcgatt cgggtcatat caagcaaatt gttgatgacg cggtgtagac gccggacggc
     5041 tagttggacc tcgcgcagag tcctttgctt tttttcatcg ccctcgatct gtcgtgctat
     5101 cgcaccgatg cccgtctgga tagcggagag cggcgtcttc agctcgtgcg aaacgctctg
     5161 cagcaacgca tgcctgaggt gctccgatgc ttcgaggatt tctgcgcgct tgaacgcttc
     5221 aacaatgtga tttttttcaa gcaccagacc gataagcacc gcgaaagctt ctagtaaatc
     5281 catctcactt gggtcaaaag gcttgcccgc cggcggacga accgagagca cgcccataac
     5341 cgccgtgcgg ccctgcagcg ggatgtgcag cgcctcggcc tccgaaaagg actcggtaaa
     5401 cttaccggcc ggcatgtgac ggctgaaaac ccatccggcc acactttttt ctgcgtccga
     5461 gagcgcaaag gagctgactg catgggggag cttcgaaagc gtgtgatctt tttgccgcac
     5521 gagaagtgct gtgttcgctg cgaaaattga atcgatgaga tttacggctg cgccaagccc
     5581 agtgtttaga tccgtcgcaa atgcggcttg ctgcgcgagc tcgtaaagag ccgccgtgcg
     5641 acgctcccgt tgtcgctcgg ttatttcgct ccggcgcacg cggctagtca gatgccccat
     5701 agccaaggcc accgcgataa agaccacaaa catgatccca tcgtggaatt tctggacata
     5761 gattgtgaaa taaggtggta caaaaaggaa attccacaaa acagcgcttg ttacagctac
     5821 ggttaaaaca ggtccgcggc tcaacttcag tcccagaaga acaactaaga gcaggtagag
     5881 cagggcgaca gcttgataac ccgtgaaggg ctcaatgagc caagcgacca ccgtgaatgt
     5941 aagaatgagt aacccggcta aaaagtattc gttaacggtt gatgccaata gaacccagcg
     6001 ccggattatg tcacttcttg accggcatcc tcgggattga gcaaccatcc ctgggtcctg
     6061 tgaggctatt atcatatgaa gaattatatc tggtttggtc tacaggatct aaggacgcga
     6121 ttcaggagcc acatcatcca aaaggaacgg attgcggccg atttataaag aagccggcag
     6181 ctcacgcggt actcggctgc tggctgccgg aaataaacac tcggattggt aagatcatca
     6241 tttccaaccc ctcgaattgg agccgctgct ctaaggtaaa aggcgtgtgg ttatgaagca
     6301 gacgcgttaa aaagttctcc tcttgaaaca ccagtttccc ggcgaaaaac actgatctgg
     6361 gaaactcctt ggctacggac ttacagagtt tctccaactc ttccatgagg tcaattccca
     6421 acgcataacg atactccgcg taccagccca agcagttggc aaagtccacg aagctttgca
     6481 gatcgtcttc tttcattttg cgtaagctct ctatttcgct aaaccccttg aatcgtcccg
     6541 agtcaatcac tccgatcgag acaaaaacta ggttcttgaa gtggttcgga aaaagccggc
     6601 ctatgctaag gagcgaatga atcgccaagc catcgaagtc tcgtacaatt agaaccgcag
     6661 tgggcgcatc ggcgttcttt gccggcactg gatccttcag gtccggctga aacgggatgt
     6721 tcatcagtgt gtcatcgagc ctcttcagcg cgctgcctac gcgatcgtaa tgtagtcgaa
     6781 ccccgtaaca gagaacaatg aagctcactg tcaccagaac tgtgatccat cccccttcag
     6841 cgaattttag agttacagtg acgatgagaa tggtggcggt gaacgacaaa ccgagaccat
     6901 ttacaaacag cctgcgcagc caaagcttct cggtatgtct tacttcccac cagtggcggc
     6961 acattccaag ctgagaaatg ctaaaggtca aaaatacatt gatcgcatac atgaccacca
     7021 gcagccttac gtcaccgccg gtgtataaaa gaaaaacgag cgaggctaga cccatgaacc
     7081 agatgccata ccgggtaacc aagtgatcgc ttagatgagc aaagcgatga ggcacccagg
     7141 agtcgatcgc catacttgaa agcactcgcg gtccgtcgat gtaaccagcc tgggccgcga
     7201 caatgaggat ggctgcttca gtgatgagag tgatgattac gagaaggtaa gccaactcgg
     7261 gtgaaaagaa acctccgtag acttttgcaa ataggctggc attcagagtt ttgcccgttt
     7321 catgtgagac ctgattgagc aaatagcaga gcagcaaacc gcttgctgta aaggcgagcg
     7381 acgtggccat atagagcatg gtttttttgc ccgtctcgac cctcggttcc cgtaacgcct
     7441 gcaggccatt gcttacagct tcgatgccgg tataggtgcc gccgccaaga ctataggcgt
     7501 ggagaacgat gaacagagtt gcccacaagc ccaagccttg aatatcgcca atggtctttt
     7561 gatacgtagc cgaggcaata agaggaactt ccgcggcctt tgggatgatg cctaggccaa
     7621 ttgcaaaaat gtgcgcgacg acaaacagaa ggaaaatggg cagcaaaaag agaactgact
     7681 ctttggtgcc gcgaagatta agatagatga gaaaaaagat caggctaaat tctaatgcca
     7741 gcttaaaccc atgggtcgaa attggcagga agctaaaaat ctgatcgccc gcgctggcga
     7801 ctgaaatagt gatcgtcagc atgtagtcta caatcaaggc cgagccggac accaacccga
     7861 ccttgggtcc caaaagtttt gtcgcgacca ggtaaccgcc tccgcccgtc ggaaaaagct
     7921 cgatgatgtg actgtaactt gcggaaatga caaaaacggt tagcgccatg agaagcgcta
     7981 atgggagcgc gaggaagaaa tgttctccaa gagccagata ggcttcttca ggaccatacg
     8041 ctgatgagct taggccgtcc gcaccaaggc ccacccaggc aaggaaggcg ac
//
LOCUS       DASUTG010000137         9556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_21662, whole genome shotgun sequence.
ACCESSION   DASUTG010000137 DASUTG010000000
VERSION     DASUTG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21662"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF089_03085"
     CDS             <1..936
                     /locus_tag="VF089_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6172973.1"
                     /translation="PVVQTFFVDERIKSQFQLNGVVTVVDSKHIALQIDHSTEAREQI
                     AFADLVLLNKLDLISPEDLQELEYKIKNLNRAARIYQTRNSDVEIAKVLDLRSLDIEA
                     KAQEHDDNHKHTDDISTVSIVTPGDLDGVKLSQWFRQLLAEFGQSIMRMKGILNLRND
                     PDQFVFQGVHLLFEGRPGRAWAEGEERTNRLVFIGRNLDGEKITNGFQACLTTEDGNG
                     SAQVDPFGQKIDVSTFVFDQIRYWVQTILEFPPDAPIVIKEVPCVKEGCPPVETAIMV
                     FLKNEPPRVFKILARVHEVTFDHVYNLIENPLPCC"
     gene            complement(981..1745)
                     /locus_tag="VF089_03090"
     CDS             complement(981..1745)
                     /locus_tag="VF089_03090"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX6172974.1"
                     /translation="MILSLALTSMFFATLSIIGRPEASAASTLQASAEQTVVEVFTIP
                     DESSLVIELVKDGQSLSPMAEITGAGGLKWFMVKTGTGNIGWIKAGENNTAKKIDGHF
                     RSLPIENSVIRPASPAESTSNAPPKGARAIPVKVSGSKVIVSVNFNNRTAANLLLDTG
                     ASRTMISSRIAKTLRLQSLGSGRGYGIGGVVNVSTARVESVKLGEMERQNLLVMIHDF
                     SPDPSYEGLLGFDFLSHFHMSLDLQKALLVLTPRKG"
     gene            2544..2729
                     /locus_tag="VF089_03095"
     CDS             2544..2729
                     /locus_tag="VF089_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172975.1"
                     /translation="MILDWLKVPENQQEIAKLCGDVNPDHVHGATAQHIPRLHELSDQ
                     TFCEFIALFGLERSAAG"
     gene            complement(2763..4178)
                     /locus_tag="VF089_03100"
     CDS             complement(2763..4178)
                     /locus_tag="VF089_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6172976.1"
                     /translation="MSKSVCRTLSIIFLMTPLLVSLSGNDFAAEGTLPGSTLEQEAVS
                     LLSRYIQIDTTNPPGNEIKAARFFKEIFDREGIEARIIESAPGRGNIYARLPGNGAKK
                     AIMLLNHMDVVPADANLWKEAPFGGTVKDGYIWGRGALDMKGPAIAELMTLLALKRKK
                     VALRRDVVFLGTADEEAGGALGAGFLLEKHPDLFANVGVVLNEGGGVRLGKDGKVQSY
                     SVSVAEKTPLWLRLIATGAPGHGSTPVRDAAVDKLVLALGRLVAYQTPIKVVPEVQKF
                     FADTAAGEPESRRRQYQDLQTALKDPAFVADFTSDPRNNASVRNTISVTRLRASDKVN
                     VISTQASADIDVRLLPGEDPQSFIGEVRRVIGDEDIKIEVLLSFPAAASPADPEAMKA
                     IQELAKAHDNDAPVVAPLVRGFTDCHFFRAKGISCLGFLPLRNIPSEGLVHGLNERIS
                     LDSLRSGVRSLYELVHKLAAE"
     gene            complement(4222..5139)
                     /gene="miaA"
                     /locus_tag="VF089_03105"
     CDS             complement(4222..5139)
                     /gene="miaA"
                     /locus_tag="VF089_03105"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6172977.1"
                     /translation="MKPKLVIVLGPTAVGKSDMVLDLALQFDAEIISADSQQVYRHMD
                     IGTAKPSREQREKIPHHLIDVVEPDEEFNAAMYRKLATEIAYNLSDRGKRIIVCGGTG
                     LYIKALTKGLFAGPGGDPQIRQALEWELEEHGIGSLYQRLEQIDPESAARIHPHDRQR
                     IGRALEVYHATGRRISEWQSEHGFNDLLFDALKVGLERQRSELYNRIDRRCEFMIQSG
                     LLGEVNKLVEMGYGLDLKPMQSVGYRQMGLFLRGRMSLDNAVQLMKRDTRRLAKRQLT
                     WFRNDREIRWFNPESQRGNIEDAVRKFFD"
     gene            complement(5194..6933)
                     /gene="mutL"
                     /locus_tag="VF089_03110"
     CDS             complement(5194..6933)
                     /gene="mutL"
                     /locus_tag="VF089_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX6172978.1"
                     /translation="MKIQVLSREMASRIAAGEVVERPASVVKELLENSIDAAATEITI
                     SIEKSGTSLIRVSDDGEGMAPEDLPLALERHATSKLRSDEDLFQISTLGFRGEALPSI
                     ASVSRFEITSRQAGYDSAYRLCVDGGNKTSAQMAAAATGTTIELRDLFYNVPARRKFL
                     KSLPTEFSHICDIVNRLALAYPKIHFRLEHNGKTLIDFVAVRELHERLNQVLGREVAD
                     SLRPFEHTQGSLSVTGYLSSAPTAFPNSRHLMTFVNRRYVRDKILTHAVLHGYETLLM
                     KSRYPAVVLLLDIPYDEVDVNVHPAKYEVRFRRQSDVHDAVARAIRQALRVEAQQPSA
                     RSIVPWRQDYSASEAVLPYSTSVSTFEQSLVSESSREVFLVPNRSNQIRSGFFSSLSV
                     LGQILGCYLVCSSAQGLALIDQHAAHERVAFERLRKQMRAHTVERQTLLLHQTIELSA
                     GEMMLLEKQLDALERYGFTLEPFGPSSYAITAIPALLPEGDYRPVVRQMVAELAEIDQ
                     SEELGQHLEERLATIACHSVIRANRQLEMHEMRALLRDLDDTDFATQCPHGRPVLLEF
                     SREELDRMFKRLL"
     gene            complement(7038..7325)
                     /locus_tag="VF089_03115"
     CDS             complement(7038..7325)
                     /locus_tag="VF089_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172979.1"
                     /translation="MAKRKRRKRSALKTILLFVLTPLIVWVLAFVVWLYWNEIANSLA
                     PVEDHSEPTAKTLRKLNKDESRSRSNETRAKEKILDADRKKLDEIIRKENR"
     gene            complement(7465..8415)
                     /gene="glpX"
                     /locus_tag="VF089_03120"
     CDS             complement(7465..8415)
                     /gene="glpX"
                     /locus_tag="VF089_03120"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX6172980.1"
                     /translation="MDRNLALEAVRVTEAAALSCARLTGRGDEKAADQAAVDAMRRAF
                     DALPIDGTVVIGEGERDEAPMLYIGEKVGSGGPKVDIALDPLEGTTICATGAPNALAV
                     IAMADGGNLLHCPDTYMEKIAAGPAGKGVVDLNKTPTENLHALAEAKRCRVEDLTVII
                     LSRPRHEALIKEVRQAGARIRLIGDGDVSAAIATTKPETGIDLLLGIGGAPEGVLAAA
                     ALRCVGGEFQGRLAPRNNEEIERAKKMGVGDIKKKFSIEELAAGDVMFAATGVTDGDY
                     LDGVHFFPGGATTQSVVMRSKTRTIRIINATHYFDHKPNY"
     gene            complement(8400..>9556)
                     /locus_tag="VF089_03125"
     CDS             complement(8400..>9556)
                     /locus_tag="VF089_03125"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX6172981.1"
                     /translation="TPLEAARTPNMDWLAARGVFGLAHVIPKGFPPGSDVGNMSIMGY
                     DPELYHTGRSPLEAASMGVTLGPKDIAFRCNLVTFGGSGSDTVMEDFTSGHITTEEAR
                     TIIRDLASTLGDDVIEFFPGVSYRHLMVWRGGTDKMETTPPHDITDQKISGFMPKGDG
                     ANRLLELMDASRAILADHPVNKTRQGQGQRRATSIWLWGQGRAPALPPLTKRFGIKGG
                     VISAVDIIHGLGVYAGLQRIDVPGITGFLDTNYRGKGEYGVKSLENNDFVFIHVEATD
                     EAGHMGDVEKKIQALEDFDEMVVGTVLKGMAHRKDWRVLLMPDHPTAIALKTHVSDPV
                     PFVLYSPQEPRNNGSVGYNEKDAARTGVVAKQAFKLMEALIEGRQTWTEI"
BASE COUNT         2126 a         2709 c         2503 g         2218 t
ORIGIN      
        1 ccggtcgtac aaacgttctt cgtcgatgag cgaataaaaa gccaatttca actcaatgga
       61 gtcgtaacgg tggtcgattc caagcatatc gcgctacaaa tcgatcactc aactgaggcc
      121 cgagaacaaa tcgcttttgc cgacctagtg ctacttaata aattggatct aatcagcccg
      181 gaagacctgc aggagctcga atataaaatc aaaaatctga accgggcggc gcgcatttac
      241 cagaccagga actccgacgt ggaaatcgcc aaagtcttgg atcttcgcag tctcgacatc
      301 gaagccaagg cgcaagagca tgatgataac cacaagcaca cggatgacat ttccactgtg
      361 tcaatcgtaa cgccggggga tctcgatggg gttaagctca gtcagtggtt tcgccaactg
      421 ctcgccgagt tcggccagag tatcatgcgt atgaagggca ttctcaacct tcgcaacgac
      481 ccagaccagt tcgtgttcca gggtgttcat ctattgttcg aaggccgtcc tggacgggct
      541 tgggccgaag gcgaggagcg gaccaatcgc ttggtgttta tcggccggaa tctggatggt
      601 gaaaagatta ccaacggctt ccaagcttgc ctcactacgg aggacggcaa cggttcagcc
      661 caggtcgacc cgttcggcca aaagatcgat gtctcgacct ttgtctttga tcagatccgt
      721 tactgggtgc agacgattct ggaattccca cccgacgcgc ccatcgttat caaagaagtg
      781 ccctgcgtca aagaaggttg tcctccggtg gagactgcca tcatggtgtt cctcaaaaac
      841 gagccgccgc gcgtttttaa aattctcgct cgtgtccacg aggtcacctt cgatcacgtt
      901 tataacctga ttgaaaaccc gctcccgtgc tgctgatcgc cggctgcgac ggttcaccta
      961 cggcgccgca gctttagtcg ttagcctttt cgcggcgtca gcactagcag cgctttttgt
     1021 aaatccaatg acatatggaa gtggctcaag aagtcgaatc ccagtaagcc ctcgtaactg
     1081 ggatcaggtg aaaaatcatg aatcatcact agcaaattct gcctctccat ttctccaagc
     1141 ttgactgatt caacgcgcgc tgtggagaca ttcactacgc cgccgattcc atatccccta
     1201 ccagagccga gcgactgaag ccgaagcgtc ttcgcgatcc gcgaagatat catggtccgg
     1261 gaagcaccgg tgtccagtag caggttggcg gcagtacggt tattaaaatt taccgaaaca
     1321 atcactttcg agccgctcac cttaacagga atagcccggg cgcctttcgg cggcgcgttc
     1381 gaagtcgatt cagctgggct agccgggcga ataaccgagt tttcaattgg caaggaacga
     1441 aaatggccgt cgattttttt tgccgtatta ttctcgcccg ctttgatcca accgatgttg
     1501 cccgtgccgg tcttcaccat aaaccatttc aagccgccgg ccccggttat ttccgccatt
     1561 ggcgaaaggg attgtccatc tttaaccaac tcgatgacaa gggatgactc gtcgggaatt
     1621 gtgaagacct cgactactgt ttgctctgcg gacgcttgca aggtcgaggc ggccgaggcc
     1681 tctggtcgcc cgattataga aagcgtcgcg aaaaacatcg aagttaaggc tagcgagagg
     1741 atcattggct aggtatcctt gcccacgata gttgcatggt aataagctgc ttgattcaat
     1801 gagcagcatt atagtccaca gattcctcaa ccgtcagctt tttgtttacc gacacatagt
     1861 caaactgccg ctgcaatcct gagatgcttg aagctaactc agccgtcaat gctcaacccg
     1921 tgcgcgttcg ccgagattta acaatgtctc tttgggcagg tataatttac cggcgagcct
     1981 gaacggatgt ttcaaaatga gaaagctttt agtcgtccag catgtcgcac atgagatgct
     2041 cggcactcta aacccactgc tgaagcgcgc cggctttcgt atccgttacg tcaacttcgg
     2101 acgccagccg gatacgcatc cgagcttgga aggatatcgt ggactcgtgg tgcttggcgg
     2161 ccccatgagt gtttacgaag atcgtcggtt tccccacctt ataactgaaa tgaaattgat
     2221 aagatgccct gcaacgagat ctgccggtac tcggtatctg cctcggcgcg caactgatcg
     2281 ccaaaacact gggggcggat gtttatcgaa acaaggagaa ggaaatcggc tggtatgatt
     2341 tacacgtcac gccgcaagcg tcgcaggaat ccattctaaa gtcgctcggt gccaccgaga
     2401 aagttttcca gtggcatgga gatacttttg atatcccggc cggcgcggtt catctcgccg
     2461 catcccctct ttgcgtcaac caagcgttta gctatgggga aaaggtttac ggcttacagt
     2521 ttcacctcga agccgacgag gcgatgatcc tggattggct gaaggtgcca gaaaaccagc
     2581 aagagatcgc gaagctttgt ggtgacgtca atccggatca cgtgcatggg gcaacagcgc
     2641 agcatatccc gcggctgcac gaactcagcg accagacgtt ctgcgaattt atcgcccttt
     2701 tcggtttgga aagatccgct gccggctaac gagttgacct ttccttcctg gacacttagc
     2761 aattattcgg cggctagctt gtgcaccagc tcgtacagcg atcgcacgcc tgagcgcaag
     2821 ctatccaggg agattctttc gttgaggccg tgcactaatc cttctgaagg gatgttgcgc
     2881 agcggcagaa agcccaaaca ggaaatccct ttggcgcgaa agaagtgaca gtcggtaaag
     2941 ccgcgcacga gcggcgccac cacgggtgca tcgttatcgt gcgcttttgc caattcctgg
     3001 attgccttca tcgcttccgg atccgccggg gaagcggccg cgggaaaaga taagagcact
     3061 tcgatcttta tatcctcgtc gccaatcacc cggcgcacct caccgataaa cgattgcgga
     3121 tcttcgccgg gcagcagtcg aacatcgatg tcggctgatg cctgggtcga aatgacgttg
     3181 accttatctg acgcccgcag ccgggtgacc gagatggtat ttcgcacgct ggcgttgtta
     3241 cgcggatcac tagtgaaatc cgcgacaaag gccggatctt taagcgcggt ttgtaagtct
     3301 tggtactgcc ggcggcggct ctcgggctct ccagcggcgg tgtcggcgaa gaacttttgc
     3361 acctccggca caactttaat cggcgtttga taagccacaa gccggcccag ggcaagcacc
     3421 aacttatcga ccgctgcatc tcttaccggc gtcgagccat ggcccggtgc gccagtggcg
     3481 atcagccgca accagagcgg ggtcttttcc gcgacgctga cgctataact ctgcaccttg
     3541 ccatctttgc ccaagcgtac tccgccacct tcgttcaaaa ccacgccgac atttgcaaac
     3601 aagtccggat gcttttcgag taaaaacccg gctcctaggg cgccgccggc ctcttcatcg
     3661 gcagtgccga gaaaaaccac gtcgcgccgc agggccacct ttttgcgctt gagcgccagg
     3721 agggtcatca gctcggcgat agcggggcct ttcatgtcga gggcgccgcg accccaaatg
     3781 tatccgtcct tgacagtccc gccgaacggc gcttccttcc acaggttggc gtccgccggc
     3841 accacatcca tgtgattcag cagcattatt gctttcttcg cgccgttgcc cggcaatctg
     3901 gcgtagatgt tgccgcgtcc cggggcagac tcgatgattc gcgcttcgat gccttctctg
     3961 tcgaagatct ccttaaaaaa tcgcgccgcc ttaatttcat tcccaggtgg attggtggtg
     4021 tctatttgaa tatagcggct cagcagtgac actgcttctt gttccagcgt gctgccgggc
     4081 aaagttcctt ctgctgcgaa gtcgttgccg ctcaaagaaa caagcaaggg cgtcattaaa
     4141 aagattatcg atagggtgcg gcacacggat ttactcacaa tccctccttc aactggaggc
     4201 gttcctgaac caaagcaagg atcagtcaaa aaattttctt acggcatctt cgatgttgcc
     4261 gcgttggctt tccggattga accagcgaat ttcgcggtca ttacgaaacc aggtgagctg
     4321 ccgcttggct aaccgccgcg tgtctctctt catcagttgc accgcgttgt ctagagacat
     4381 ccgaccccgt agaaacaacc ccatctggcg ataaccgaca ctttgcattg gttttaaatc
     4441 caagccatag cccatttcga cgagcttatt gacttcgcct aacaagccgg actggatcat
     4501 gaattcgcag cgccggtcga ttcgattata gagttcacta cgttggcgct ccagaccgac
     4561 tttaagcgca tcaaagagca aatcattgaa accatgctca ctctgccact cactgattct
     4621 tcgcccggtc gcatggtaaa cttcgagcgc ccggccgatt cgctgccggt cgtgcgggtg
     4681 gatccgtgct gccgactctg ggtcgatctg ttccagtcgc tgataaagcg aacctattcc
     4741 atgctcctct agctcccact caagcgcttg tctaatctgt ggatctcctc ccggtccggc
     4801 gaataatcct tttgtaagag ctttgatgta gagtccggta ccgccgcaaa caataatcct
     4861 cttgccgcga tcagataggt tgtatgcaat ctcggtggct agctttcgat acatggcagc
     4921 gttgaattcc tcatcgggct caacgacatc gatcaaatga tgtggaatct tttctctttg
     4981 ctctctggat ggtttagcgg tgccaatgtc catgtggcgg tagacctgtt gcgagtcagc
     5041 gctgatgatc tctgcgtcaa attgtaacgc gagatcgagt accatatcgc ttttgccgac
     5101 ggctgtggga ccgagaacaa tgacgagttt gggtttcacg gcagcgaagt agccagattg
     5161 cgggaacggg tgaccaaaga atggcccggc aagctataac agccgcttga acatccggtc
     5221 aagctcttca cggctgaact caagcaggac cggccggccg tgggggcatt gagtcgcgaa
     5281 gtcggtatcg tcgagatccc gcaatagggc gcgcatctcg tgcatttcca actggcgatt
     5341 agcccggatc acgctgtggc aggcgatggt cgccaggcgc tcttccaagt gctgacccag
     5401 ctcctcggac tggtctatct ctgccaattc cgccaccatc tggcgcacca ccggccggta
     5461 gtcgccttcc ggcagtaacg ccgggatcgc ggtgatcgca taagagctcg gtccaaaggg
     5521 ctccagtgta aagccatagc gttccaaggc atcgagctgt ttttccaata gcatcatttc
     5581 accagccgac aattcgatcg tctgatgaag caacagtgtt tgtcgttcga ctgtatgggc
     5641 gcgcatctgc tttcgtagcc gttcaaaggc cacccgttcg tgcgccgcat gttggtcgat
     5701 cagcgccaag ccctgagcag atgaacaaac caaataacag ccgagaattt ggcccagcac
     5761 actgagtgac gaaaaaaatc ctgagcgtat ttgatttgat cggttcggaa caagaaaaac
     5821 ttcccggctc gattctgaaa ccaacgactg ctcgaaagta gagactgaag tcgagtacgg
     5881 caaaacggct tccgatgcag aataatcttg ccgccatgga acaatgcttc tggccgacgg
     5941 ctgttgagcc tcgaccctga gagcctggcg tatcgcgcgg gcgaccgcat catgcacgtc
     6001 ggactgacga cgaaatctca cttcgtactt ggccgggtgg acgttgacgt ccacctcgtc
     6061 gtagggaata tccaacagca gcaccaccgc ggggtatcgg cttttcatca aaagagtttc
     6121 gtagccatgc agaacagcat gtgtgaggat cttatcgcga acataccggc ggtttacaaa
     6181 cgtcatcaga tgacgcgagt tgggaaatgc tgtcggagct gaactgaggt agcccgtgac
     6241 agaaagggag ccctgggtgt gctcgaacgg tcgtaaagag tcggcgactt cccttcctaa
     6301 gacttggttc aagcgttcgt gcaattcgcg cactgcgaca aaatcgatca acgtttttcc
     6361 gttatgttcc agacggaaat gaattttcgg ataggcgagc gccagccggt taacgatatc
     6421 gcaaatatgg ctgaattctg tagggagaga tttcaaaaac tttcgccggg caggaacatt
     6481 gtaaaacaaa tctcgcagct cgatcgtcgt cccggttgca gcggctgcca tctgggccga
     6541 ggttttgttt ccgccgtcaa cgcacaatcg gtatgcggaa tcataaccgg cctgccggga
     6601 agtaatctcg aaccgagaga ccgaagcgat gctcggcaag gcctcgccac gaaaacccaa
     6661 tgtcgaaatc tgaaagagat cctcgtcact gcgcagtttg ctggttgcat ggcgctcgag
     6721 cgcaagcggt aaatcttcgg gcgccattcc ttcgccgtca tcgctcaccc ggattagcga
     6781 ggtcccgctc ttctcgattg agatggtaat ctctgtggct gccgcatcga tggaattttc
     6841 cagcagctct ttgaccaccg atgccggccg ctccaccact tctcctgcgg caatacggct
     6901 cgccatttct ctcgaaagaa cctgaatctt cacttaatac ttgctccggc tttgcgcttg
     6961 tctatggcaa tagaagccgt caacttggtt tcagctttcc gaattgagcg cgctattgtg
     7021 tagtccgggc ggatgcctca gcgattttcc ttacgaatga tctcgtccag ctttttccgg
     7081 tccgcgtcca aaatcttttc ctttgcgcgg gtctcattgg accgactacg gctctcgtct
     7141 ttgtttagct tgcgcagggt cttggcggtg ggctcggaat gatcttcaac cggcgctaaa
     7201 ctatttgcga tctcgttcca atatagccaa acaacgaagg ctagcaccca aacaataaga
     7261 ggcgtaagca cgaacagaag aatggttttt agcgcgcttc ttttccttct ttttcgtttg
     7321 gccacctttg atcgatcacc taatgatttt gctgacactc tgcaggatgc ccaaaatgac
     7381 tcagaggcga gcaacaaggg tactcttcaa tacgtcgatg cgaggcgatt gagaccaacg
     7441 acacatgagt ttttcagcaa cctgctagta attcggcttg tgatcgaaat agtgggtggc
     7501 gttgataatg cgaattgtgc gagtctttga gcgcatcacc accgattgcg ttgtcgcgcc
     7561 gccggggaag aaatggacgc catcgagata atcgccatca gtgaccccgg tggccgcgaa
     7621 catcacgtca cccgcggcta attcctcgat cgaaaacttc ttcttgatgt cgccgacacc
     7681 catttttttt gcgcgctcga tttcttcatt gttgcgcgga gcgagacgac cttgaaattc
     7741 gccgccgacg caacgcagcg ccgctgctgc gagcacgcct tccggcgcgc cgccgatccc
     7801 aagcagcaga tcgatgccgg tttccggttt ggtcgtcgca atcgctgcag agacgtcgcc
     7861 atcaccgatc aggcgaatcc gcgctcccgc ttggcgcact tctttgatca gcgcttcatg
     7921 tctcggacgg gacaaaatga tcaccgtgag atcctcgacc ctgcaacgtt tagcttccgc
     7981 tagagcgtga agattctcag tcggcgtttt gttcaagtcg acaacgcctt taccagccgg
     8041 tcccgcggcg atcttctcca tgtaggtgtc cgggcagtgc agtagattgc cgccatcggc
     8101 catggcgatg accgccaaag cattgggcgc gccggtggcg caaatggtcg tgccttccag
     8161 tggatccagc gcgatgtcaa ctttcggacc gccggaaccg accttctcgc cgatataaag
     8221 catcggtgct tcgtcgcgtt caccttcgcc gatgaccacc gtgccgtcga tggggagcgc
     8281 atcaaacgcc ctacgcatcg cgtccaccgc cgcctgatct gcagcttttt catcgccacg
     8341 tccggttaaa cgagcacaac tcaaggcggc cgcttcggta actcgaaccg cttccaaagc
     8401 taaatttcgg tccacgtttg cctcccctcg attaacgcct ccataagctt gaaggcttgc
     8461 ttagcgacca cgccggttct cgcggcgtcc ttctcattgt atccgacact gccgttattg
     8521 cgaggctcct gcggagaata gagcacgaac ggcacgggat cagaaacgtg ggtcttgagc
     8581 gcgatggcag tcggatggtc gggcatcaat aagacgcgcc aatcttttct gtgggccatg
     8641 cctttgagca ccgtgccgac aaccatttca tcgaaatcct cgagagcctg aatttttttc
     8701 tccacatccc ccatatgacc ggcctcatcg gtggcttcga cgtggataaa aacaaaatca
     8761 ttgttctcga gcgatttaac gccgtactcg cctttgccgc gataattcgt atccaagaac
     8821 ccggtgatgc ccggcacatc gatccgttgg aggccggcgt agaccccgag gccgtgaatg
     8881 atatctaccg ccgagataac gccgcccttg ataccgaaac gcttggtcaa agggggcagc
     8941 gccggcgccc gcccctgtcc ccataaccag atactggtgg cccggcgctg accttgaccc
     9001 tgtcgcgttt tattgaccgg gtgatcggca agaatcgccc gcgacgcgtc catgagctcg
     9061 agcaatcgat tggcgccatc gcccttgggc ataaagccgg aaatcttttg atcggtgata
     9121 tcgtgcggcg gggtcgtttc catcttgtct gtgccgccgc gccaaaccat caggtgccgg
     9181 taactgacgc cgggaaaaaa ctcgataacg tcatcgccga gcgtgctcgc cagatctcgt
     9241 attatcgtcc gagcttcttc agtggtgata tgaccggaag taaagtcctc catcacggta
     9301 tcactgccgc tgccgccgaa ggtgaccagg ttacatcgga aggcgatatc tttcggtcca
     9361 agcgtcactc ccatgctggc ggcttccaaa ggcgaccggc cggtatgata cagctcagga
     9421 tcgtagccca tgatgctcat attgccgaca tcgctacccg ggggaaagcc tttcggaatt
     9481 acgtgagcca agccaaacac cccgcgcgcc gcgagccaat ccatattcgg tgttctggcg
     9541 gcttccagag gagtct
//
LOCUS       DASUTG010000138         5443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_21847, whole genome shotgun sequence.
ACCESSION   DASUTG010000138 DASUTG010000000
VERSION     DASUTG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21847"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF089_03130"
     CDS             <1..457
                     /locus_tag="VF089_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX6172982.1"
                     /translation="IIWLGEGVAGDDTDGHIDDIARFVNARTIVCAAEDDPKDANYAP
                     LQENLERLEGARDALGSRFDLITLPSPRPVICQGSRLPASYANFYIANEVVLVPIFDD
                     PGDQKALGILGELFSTRKAVGIGCTDVVAGLGAIHCVTQQEPQIGEPRA"
     gene            723..1850
                     /locus_tag="VF089_03135"
     CDS             723..1850
                     /locus_tag="VF089_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX6172983.1"
                     /translation="MELTKSLPLVLLFLVGLTILGWFFLCRMRALLRATQNDQSWLLM
                     QQQIDQLRMQFGQVLDNSTQLIQQQLGQMIGHVNERLRENSEILQQTHQSVGQRLDNA
                     ARVVGDVQRSLGGLEEANRKIYEVGKDIASLQEILRSPKLRGGLGEFFLEELLAQVLP
                     PQHFTTQYGFRSGEKVDAVIRLGSSLVPVDAKFPLENFKRIIDVTVDGDQARAKRQFA
                     ADVRKHIDAIATKYILPDEGTYDFALMYIPAENVYYELIIKDDSTEEKSLSYYAMSKR
                     VVPVSPNCFYAYLQAIVLGLKGMTIEQRAKEIIECLSRLQGDFTRFRDEFGLLGKHLG
                     HAQSSYQNAEKRVEQLGQKLLLANAGQAELFQTAVPDRKPV"
     gene            1881..3323
                     /locus_tag="VF089_03140"
     CDS             1881..3323
                     /locus_tag="VF089_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6172984.1"
                     /translation="MRLSLFSRLALGYLAIFLLVTAVSTYAVFQLHRFDELTDSVLKV
                     DNRILDYEKILADLLLSQSRYEQKFAISRDEALYNEFLSLRHDFNMQLATAISVGDSA
                     AGAILSRVQQDYRNYEELVVKEAALLRAKKTYPQAQFKKEKDALVDRILVWLEKLRAD
                     QQHAMSSKVRELAEAAEHAREVSVTITVACLISIVVMSLIVTRSITRPIAILKTKTRE
                     IAQGRFEGGLDIKSPPELGELAAAFNLMCERLTELERMKADFFSSMSHELRTPLTSIK
                     EGTGLLLDGVGGETTDKQRKLLAILAEESNRLIAVVNSLLDLSKMEAGMMSYEFDVVT
                     IDPLIRRAIAEITPLVEAKQIELESKVNPSLPALRLDPERILQVLRNLLGNAVKFTPK
                     GGRVSVVAASVNGNLQVSVTDSGPGVPAESLTTIFEKFSQGSHKGAHARNGTGLGLAI
                     AKNIIKSHGGEIWAESQVGRGSTFVFVLPC"
     gene            3389..3877
                     /locus_tag="VF089_03145"
     CDS             3389..3877
                     /locus_tag="VF089_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172985.1"
                     /translation="MLEGHSLFVYGDYDGAFAKYREALALAGDHAPADAAVYNIGLIY
                     AHPSNPKGDHRKAIDLFSKVISDYAQSPWAEQARIMVGLLRQVEISKQEAEVIREAME
                     ESKEAIERSRQDAEKFRLAAEKSKQEVDRTRQEIEKSRQVLEKANQIDIEIEQKKRER
                     GK"
     gene            3890..5320
                     /locus_tag="VF089_03150"
     CDS             3890..5320
                     /locus_tag="VF089_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6172986.1"
                     /translation="MSLGKILIVDDDANLLELIKMRLESADYEVTAAREEDNALAALK
                     AGTFDLCIVDLMLAHRDGITLMEEFRAVNPEVPTIILTAHGSIQNAVEAMRRGAYSYL
                     TKPFEPGDLLLQIERALENRKLTSEIKRLKGLLHEQFDFANIIARSAKMRSVLDVVMR
                     IARLDSTVHIHGESGTGKELIAKAIHLASDRKDKPFIALNCAALPETLLESELFGHEK
                     GAFTGAVKSTRGLFTQAHGGTLFLDEIGDMPLSTQSKLLRVLQERQFYPVGSEIPTEV
                     DVRVIAATNKDLEELVKKGTFRDDLFYRIHVIPIYLPPLREHKEDIVPLVEHFLKKCS
                     QQMKKDVKGLTPEALRKLMLHDWPGNVRELENTVEYSVAMTQKEFVTEEYILQGKISF
                     SDGAQAGVNDKPTASDDALRPLKDARDAFERDYLVQVLSMTDGNVSQAAKLAGKYRAD
                     FYDLLKKHDLKADDFKKSKSAASGSS"
BASE COUNT         1366 a         1368 c         1497 g         1212 t
ORIGIN      
        1 aataatttgg ctaggcgagg gagttgcggg cgatgacacc gacggtcaca tcgatgacat
       61 tgcccggttc gtcaatgcca ggaccatcgt ctgcgctgcc gaagacgacc cgaaagatgc
      121 gaattacgct ccgctgcaag agaatctcga gcggctcgag ggagcacgcg atgctcttgg
      181 tagcaggttt gatctgatca ctttgccgtc tccgcgacct gtgatttgcc agggatcacg
      241 attacccgcg agctatgcca atttttatat cgccaatgaa gtagttctcg tgcctatttt
      301 cgacgatcct ggtgatcaaa aagcgctggg aattttaggt gagttgtttt cgacccgaaa
      361 agcggtcgga attggttgca ccgacgtcgt tgcaggtctt ggcgccatcc attgcgtgac
      421 ccagcaagag ccgcagatcg gtgagccgag ggcttgacaa tctcttcatc ccgaacttat
      481 tcttagcttt gctatggggc ggctcatcca aatccgagac ctgcatcagg cacgacgtcg
      541 tcgttccgag aaggtcagca tggagcaatg cgttgagctg cttgagtgga acttgaaaaa
      601 aagcctcgat gattacctct cttcacctcg tgaagagcgt tcgatgagag caacgcagat
      661 tcgcaaactc agtgagattc ttgaatacgc tttgaggctt ctctaactcc acacctcgat
      721 ccgtggaact tacaaagtcc ctgccgctgg tcttgctatt ccttgtcggc ttgacgattc
      781 tcggctggtt ttttctctgc cgcatgcgcg cccttctacg agcaacacag aatgatcagt
      841 cctggttatt gatgcagcaa caaattgacc aactccgcat gcagttcggc caagtgctgg
      901 ataacagcac gcagctgatt cagcaacaac taggacagat gatcggccac gtcaatgagc
      961 gtttgcgcga gaactcagag atcttgcagc aaacacatca gagcgtcggg caaaggctgg
     1021 acaatgcggc cagagtggtt ggcgacgtgc agaggagcct cggcggactc gaggaagcca
     1081 accgtaagat ttacgaagtc ggtaaggaca tcgcttcact gcaggaaatt ctccgttcac
     1141 ccaagctgcg cggcggatta ggcgagttct ttctagagga attgttggca caggtcctgc
     1201 ccccacagca cttcacgacg caatacggtt ttcgtagcgg cgagaaagtg gacgcggtta
     1261 tcaggctggg tagctcgctg gtgccggtgg acgccaagtt tccgctggag aatttcaaaa
     1321 gaatcatcga tgtcactgtc gatggggacc aagcgcgggc gaagaggcag tttgccgccg
     1381 atgtcaggaa acatatcgac gcaatcgcga caaaatatat actaccggac gaaggaacat
     1441 acgatttcgc tctgatgtat atcccggccg agaatgtcta ttacgagctc attatcaaag
     1501 acgattcgac agaagagaaa agccttagct actatgcgat gagcaaacgc gtcgtaccgg
     1561 tttcgcctaa ctgcttttac gcatatctac aagcgattgt gctggggctt aaaggcatga
     1621 cgatagaaca gcgagcgaaa gaaatcatcg aatgtttgag ccgtttgcaa ggcgatttta
     1681 cgcgctttcg cgatgagttc ggtctgctcg gcaagcatct gggacacgcc cagtcgagct
     1741 atcaaaatgc tgagaagcgg gttgagcagt tgggtcagaa actgctgtta gccaacgccg
     1801 gacaggccga attgtttcaa accgccgtgc ccgaccgaaa acctgtctag ctgttggctc
     1861 ctcagaggcg gcgtctcgac atgcggttaa gcttgttctc ccggttggct cttggctacc
     1921 tcgccatctt ccttctggta acggcggtga gcacgtacgc cgttttccag cttcatcgtt
     1981 ttgatgagct taccgattct gtactcaagg tcgataatcg aattctcgat tacgaaaaga
     2041 ttctcgccga cctgctgttg tcgcagagcc gttatgagca gaaatttgct atcagcagag
     2101 atgaggcact gtacaacgag tttctcagtc tgaggcacga cttcaacatg caacttgcca
     2161 ccgccatttc cgtcggggac agtgcggcgg gagccatctt gagcagagtt caacaggatt
     2221 accgcaacta cgaggagttg gtggtcaaag aagcggcgct cctgcgcgcg aaaaagacct
     2281 atcctcaggc gcaattcaag aaagaaaagg acgcgctcgt cgatcgcata ctcgtgtggc
     2341 tggagaaact gagagccgat caacaacatg ccatgtctag caaagtgaga gagctggcag
     2401 aagccgcgga acacgcccgc gaggtttcgg ttaccatcac cgtggcgtgt ctgatctcga
     2461 ttgtggtgat gtcgctgatt gtcacccgga gcatcactcg accgatcgca attctaaaga
     2521 ccaagacccg agagatcgcg cagggtcgtt tcgaaggagg actagatata aaatctccgc
     2581 ccgaactcgg tgagttggcg gccgcattca atttgatgtg cgagcgattg acagagcttg
     2641 agcgcatgaa agccgacttc ttttcatcga tgtcgcacga gctgcgcacg cctttgactt
     2701 ccatcaaaga gggtacgggg ctgcttctcg acggggtcgg cggcgagacc acggacaagc
     2761 agcgaaaact tctggcgatc cttgccgagg aaagcaaccg gctgatcgct gttgtcaatt
     2821 ccctgctcga tttatctaaa atggaagcag ggatgatgag ctatgaattc gatgtggtga
     2881 ctattgatcc gctgattcgt cgcgccatcg ccgagatcac gccgctcgtg gaggccaagc
     2941 aaatcgaact agagagcaag gtgaacccgt cactgccggc gctcaggttg gaccctgaaa
     3001 gaatactcca agtcctgcgt aatttgctcg gcaatgcagt taagtttact cctaagggag
     3061 gccgggttag tgttgttgcg gcatctgtta atggcaactt gcaagtttct gtgacagact
     3121 ctggccctgg agtgccagcc gagagcttga ctaccatttt tgaaaagttt agtcagggga
     3181 gccacaaggg cgcgcatgcc agaaatggca cgggcttagg attggcaatc gctaagaata
     3241 tcatcaagtc ccatggtggt gaaatatggg cggaaagtca ggtagggcgg ggaagcacct
     3301 ttgtctttgt cttaccctgt tgagtgtatt ttcatccggc tgcagtctgc taagagattg
     3361 gcgcgagcag cgccagattc gcgactcaat gctcgagggt catagtctgt ttgtttacgg
     3421 cgattacgac ggagcgttcg ctaagtaccg cgaagcttta gcgttagcgg gggatcatgc
     3481 ccccgcggat gccgccgttt acaatatcgg actgatttat gcgcatccca gcaatcctaa
     3541 aggcgatcat cgcaaagcga tcgatttgtt cagcaaagtg atcagcgatt acgcgcaaag
     3601 cccttgggcg gagcaggcaa gaataatggt gggcctgctc cggcaggttg aaatatcgaa
     3661 acaagaagcg gaagtgatcc gggaagctat ggaagagtct aaagaagcca tcgaaagatc
     3721 ccgacaagac gcggaaaagt ttaggttggc tgctgagaag tccaagcagg aagttgatag
     3781 aaccaggcaa gagattgaga aatctcggca agttctagag aaagccaacc agatcgatat
     3841 cgaaatcgaa caaaagaagc gcgaacgagg caagtagaaa ccgccaccca tgtcgttagg
     3901 caaaatattg atcgtcgatg atgacgcaaa cctcctcgag ctgataaaga tgcggctgga
     3961 gtccgcggat tacgaggtga ctgctgcgcg tgaagaggat aatgcgctcg ccgcgcttaa
     4021 ggcggggacc tttgatcttt gcatcgttga tttgatgcta gcccaccgtg acggcataac
     4081 gctgatggaa gagttccgcg ccgtcaaccc tgaagtgccg acgatcatcc tgacggcgca
     4141 cggcagcatt caaaatgcgg tggaggcaat gcgtcgcggc gcctacagct atttgaccaa
     4201 accattcgag cctggcgacc tgttgttgca gatcgaaaga gccctggaga atcgcaagct
     4261 cacctccgag atcaagcgtc tcaagggcct tctgcacgag cagtttgatt tcgccaatat
     4321 cattgcccgc agcgccaaga tgcgatcggt tctggacgtg gtgatgcgca tcgcgagact
     4381 cgattcgacc gtccatattc acggtgaaag cggtactgga aaagaactca tcgccaaagc
     4441 catccatctc gccagcgata ggaaagataa gccgtttatc gctttgaact gcgccgcact
     4501 acccgaaact ttgctggaaa gcgagctctt cggccatgag aaaggcgctt tcaccggcgc
     4561 agttaaaagc accagggggc tttttactca agctcatggc gggacactct ttctagatga
     4621 gatcggtgac atgccgcttt caactcagtc gaaactgttg cgtgtcttac aggagcggca
     4681 attctatccc gtcgggagcg agatccccac ggaagtcgac gtgcgagtca tcgccgccac
     4741 caacaaagac ttggaagagc tcgtaaagaa gggcacgttt cgtgacgatt tattctaccg
     4801 aattcatgtt atccccattt atttgccgcc cttgcgtgaa cacaaagaag acatcgttcc
     4861 actagtcgaa catttcctga agaagtgcag ccagcagatg aagaaggacg ttaaaggctt
     4921 aacgccggag gcattgcgga agctaatgct gcacgactgg ccgggaaacg tgcgcgaact
     4981 ggaaaatacc gtcgaataca gcgtggccat gacgcagaag gagtttgtta ccgaggagta
     5041 tatccttcaa ggaaaaatct ctttctcaga cggtgcccag gccggcgtca atgataaacc
     5101 gacagcatcc gatgacgcac tgcggccttt aaaagatgcc cgcgatgcgt tcgaacgaga
     5161 ctatttagtg caggtgctct ccatgaccga tggaaatgta agtcaagccg ccaagctggc
     5221 cggtaaatat agagcggatt tttatgacct gctgaagaag cacgacctca aagcggacga
     5281 tttcaagaaa tccaagtctg ccgcgagcgg atccagctga accagacttt atccgcctca
     5341 cgctcggaca cgagtctaag cttgtctcgc gagtttggcc tccactactt gttagtttta
     5401 cactttcagc tacatttagg tcgcgcgaaa atcctacgcg tat
//
LOCUS       DASUTG010000139         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1059576, whole genome shotgun sequence.
ACCESSION   DASUTG010000139 DASUTG010000000
VERSION     DASUTG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059576"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VF089_03155"
     CDS             complement(<1..852)
                     /locus_tag="VF089_03155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6172987.1"
                     /translation="MTAHLEPERPRFFYGWYIVGVAILVNIAGTFAFSSTLSIFLKPI
                     TEELGVTRGAFSLIRTFEIGIAAMIVPLLGPWIDRHGGRGIIVIGVLMEGAGLLLSSI
                     VQSFWQFVLVRCSLVIAGEALLGSLVINVTIAQWFVRKRGRAMGIANLGTGIAKLAIP
                     LVAASLFVLVGWRGTWAVFGIVAPLLVVAPALIFIRRRPEDMGLRPDGESRQSSEVLP
                     AAGQKPSNAQPRKLLRETPWSRSEVLRLPVFWLLVVTFGIASVGIAGLNLHIFSYVTD
                     IGYSPLIA"
     gene            1102..2181
                     /locus_tag="VF089_03160"
     CDS             1102..2181
                     /locus_tag="VF089_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172988.1"
                     /translation="MLILSNEDIEKILPVGACLDVLEDAYRDLGNGMAATVPRYDVFS
                     PTKPNEFYEYKTMSGVLPKRKIAALRLNSSVVKWYEQGGSVRKDKLPVAGGDRYVGLV
                     MLFSTETGEPLAIFPDGYVQKLRVAGASAIAARHLARKKASVMALLGSGWQASAHLIT
                     LSLVRDLKKIKVFSPTAESRRRFLREWNDKVSAELVESDSAAAAIDGADIITCTTNSI
                     SALFPGELLKPGVHVSCVKPCELDAATYQRSDPLIIHWKEAKPFQIVIGGVDPQSIPD
                     VAEGWPHPLTREDTPVWNLPTLSQLVNGQHPGRTKDEAISCFCNNVGLGLQFAAVGSE
                     VLARAQEARIGREIPTDWFLESVHP"
     gene            2282..3274
                     /locus_tag="VF089_03165"
     CDS             2282..3274
                     /locus_tag="VF089_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6172989.1"
                     /translation="MKIKIALVTLLLACGTKPWSAMGQTVKLNVAYPTTVGSMAVLWV
                     TKEAHIFEKHGLQVELIYIGGSSKVVQAMLAKEIPIAEIAIPAVIQANLAGADLVMLA
                     GPNHKPGQKIMVRPEIRKAEELKGKKIGVTRFGTSDDFLLRYVLGQWGIAPDREVALI
                     QMGGSQETLAGMIAKSVDGGLLSSPLHLQAVKLGFSMLADLSAIGVDYQGAGVVTTQR
                     YLRENPETIRRYLRAYVEGLHRLKTDKAFSMKVIGKYSRISEADALEETYQHYAVKVM
                     PRVPYPTTKGIQMVLDEMSSRNPKAKGLQPGSFIDVANLRELEQSGFIKRLYGE"
BASE COUNT          848 a          912 c          963 g          716 t
ORIGIN      
        1 cgcaatcagc ggcgagtagc cgatatccgt gacataagaa aagatgtgca gattgagacc
       61 agcgatgccg acactggcga taccgaaggt gactacgagt agccaaaaaa ccggcaatcg
      121 caagacctcc gaccggctcc acggggtttc gcgtagaagt tttcttggtt gcgcattcga
      181 cggcttctga ccggccgccg gcaatacttc agagctttgc cgcgactctc catccggccg
      241 aagccccata tcttcgggtc gtctgcgtat gaagatcagc gccggcgcga ccaccagaag
      301 aggagcgacg atgccgaata ccgcccaggt gccgcgccaa ccgacgagga caaacaacga
      361 tgctgcgacc agagggatag cgagcttggc aatgccggtt ccgagattgg cgatgcccat
      421 ggcgcgtccg cgcttgcgca cgaaccactg cgcgatcgtg acgttgatga ccagtgagcc
      481 cagtaaagct tcgccggcga ttacaagaga gcagcgcacc agaacgaatt gccaaaaact
      541 ttggacaata cttgaaagca acaagccggc gccttccatc agcactccga tgacgataat
      601 tccacgcccg ccgtgccgat cgatccaagg tcctagcagc ggcacgatca tagccgcgat
      661 gccgatctcg aaagtgcgga tcagcgaaaa cgcgcctcgc gtaacaccta gttcctcggt
      721 gatcggtttg agaaaaatgc tcaacgtgct cgaaaacgca aatgtcccgg caatgttgac
      781 caggattgcg acgccgacga tgtaccaacc atagaagaat cgcggccgtt ccggctctag
      841 atgtgcggtc atggtcgagc gaggattata ttggcgccgc cggtaaaggc gtgggaaatt
      901 tgagagccgt agataccgtc gcgaaaggac cgcattcggt gtccacttgc cgaatcaacc
      961 tatacgcaat tcatcgagaa atattcggtg gtctgatatg acaacagcac gagcctgctg
     1021 tcaacaaagg ggcttgtgaa aaacgaagtt gaaaaaatcg agccatcaca ataactatgg
     1081 gcgcacgcca ggaggtaact aatgctcatc ctatcgaatg aagatatcga aaagattctc
     1141 ccagtgggcg cttgtctcga cgtgctagag gatgcctatc gtgatctcgg caatggtatg
     1201 gcggcgacgg tgccgcgcta tgatgttttc agtcccacca agcccaacga attttacgaa
     1261 tacaaaacca tgagcggtgt tctgccgaaa cgcaagatcg ccgccttgcg cctcaattca
     1321 agcgtggtga agtggtacga acagggcggc agcgtgcgaa aagacaagct tccggttgct
     1381 ggcggcgatc gctacgttgg cttggtcatg cttttcagca ctgaaaccgg tgagcctttg
     1441 gcgattttcc ccgatggcta tgtgcagaaa ctccgtgtcg ccggcgccag cgccatcgcc
     1501 gcgcgacatc tcgcgcgcaa aaaggcatcg gtgatggcac tgctgggaag tggttggcaa
     1561 gcgagcgctc atttgattac gcttagcctg gtccgcgatc tcaagaaaat caaagttttc
     1621 agccccaccg ccgagtcgcg gcggcgcttc ttgcgcgagt ggaacgacaa ggtttccgcg
     1681 gaacttgtcg aatccgatag cgcggccgca gccatcgatg gcgcggatat catcacctgc
     1741 acgactaatt caatttccgc tctgtttccc ggtgagctgc tcaagccggg agtgcatgtc
     1801 tcgtgcgtca aaccttgcga gctggatgcc gcgacgtacc agcgatcgga tccattgatt
     1861 atccactgga aagaggccaa accatttcaa atcgtcatcg gcggcgtcga tccgcaatcg
     1921 attccggatg tcgctgaagg ctggccgcat ccgcttacac gggaagatac tcccgtgtgg
     1981 aacttgccga cgctttccca gttagtgaac ggtcagcatc cggggagaac caaagacgag
     2041 gcgatcagtt gcttttgtaa caacgtcggg cttggcttac agttcgccgc cgtcggcagc
     2101 gaagtgttgg cacgtgccca agaggcccga atagggcggg agattccgac cgattggttc
     2161 ttggaatcgg ttcacccata gtgcgtgtcc agtttcagcg ttagctgttc gctcaagcct
     2221 acttacaccc gatgtgaaat ccaaaccaaa agtcccagac ccaactcatg acgaggtgtt
     2281 cgtgaaaatc aagatagcac tcgtaacatt gctgttagcc tgtggcacca agccttggtc
     2341 tgcgatggga cagaccgtta aacttaacgt tgcttatccg accactgtcg gttctatggc
     2401 ggtgctgtgg gtcaccaaag aagcgcacat tttcgaaaag cacggactgc aggtagagct
     2461 gatctacatc ggcggcagct ccaaggtggt tcaggcgatg ctggctaaag agattccaat
     2521 cgctgagatc gcaattccag cggtgattca agccaacctg gcaggcgctg atctggtgat
     2581 gcttgcgggg ccgaatcaca agcctgggca gaaaatcatg gttaggccgg agatcaggaa
     2641 agccgaagag ctaaaaggta agaaaatcgg cgtgacgcgc ttcggcacat ccgacgattt
     2701 tcttctccgt tacgtgttgg gtcaatgggg gattgcgcct gaccgtgagg tggcgctgat
     2761 ccagatgggc ggttcacaag agacgttggc cggaatgatc gccaaaagtg ttgacggcgg
     2821 cttactctca tcgcctctac atctgcaggc cgtcaagctg ggtttttcta tgttggcgga
     2881 cctaagcgcc attggggtcg attatcaggg cgccggtgta gtgaccacac agcgctatct
     2941 gcgtgaaaac ccggaaacta tccgccgcta cttgcgcgca tacgtcgaag gtttgcaccg
     3001 attgaagact gacaaagcct tttcaatgaa ggtgataggt aagtattcgc gtatcagcga
     3061 agccgatgcc ttagaagaga cctaccagca ttatgccgtc aaagtgatgc cgcgggtacc
     3121 gtatccaacg accaaaggca ttcagatggt gctagatgag atgagctcgc gaaatcccaa
     3181 agctaaaggt cttcagccgg gtagttttat agatgttgca aatttaagag agctagagca
     3241 gagcgggttt atcaaacgac tctatggtga atgacctgcc gcgtgactga tctaatcgga
     3301 gatcgaagtg atcagaacaa agacgtctgt taccaccgtc attagaccag agaccaggat
     3361 cgatcgtaag cccttccccc aagaatcgtc gatgagcgcg agctttgctt cttgattcac
     3421 atgcgtaaac tacgcagac
//
LOCUS       DASUTG010000140         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_318494, whole genome shotgun sequence.
ACCESSION   DASUTG010000140 DASUTG010000000
VERSION     DASUTG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318494"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF089_03170"
     CDS             <1..526
                     /locus_tag="VF089_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6172990.1"
                     /translation="VEYEIVKGNNTYEVQIDFDKGGKASEIDVAPNLWRTDATKAVLL
                     GRKAETPKGAVANPDRYSDRDRRKAYDSESEKLEKALKTGENKESYRRQIEKMGYKIT
                     SVNADKPDYVEYEVVKGDDTFEVQIDFKDGKATKVDVAPNIWQAEATDKALSRREEKA
                     EKREERAEKRQEKR"
     gene            686..838
                     /locus_tag="VF089_03175"
     CDS             686..838
                     /locus_tag="VF089_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX6172991.1"
                     /translation="MSTLLIIILILLLIGALPTWPYSGGWGYYPSGGLGLILLIIIIL
                     VLMGRL"
     gene            885..2462
                     /locus_tag="VF089_03180"
     CDS             885..2462
                     /locus_tag="VF089_03180"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX6172992.1"
                     /translation="MKRALVWLLAIFGLLVVLLVIAAFFIDEPLRRRTEAKINASLKG
                     YTVRIEKLDFHPIGFSLDLENSVITQDANPEPAVAEIPNLTASVNWRALLSGHVVADF
                     EINNPKLFLNFKQSENEIKDETPIEERGWQDAVQAIYPLKINEFVITGGALTYVDQGQ
                     YRPLELTDVNLRAYNIRNVRSQEGEYPSEVYIEGTVFKNGKIVLEGNADFLAEPHVAI
                     KAAIQAEQIELDYFRPITERYNFSVRSGVLSTKGSIEYAKNKRILSVPTLNVQKLVAD
                     YVHTKPASSPTKELSKKADKVIREHSNEPTLELDVNDVNITDSELGIVNKASKPQYRL
                     FVTGAQVKIQNLSNQGEDGTATITANGRFMNSGKTRLIARLRPKGKSPNFDLQLAIDQ
                     SELKALNPLLRAYIGADVVAGQFSFYSEIAVRNGSINGYVKPLFKEIDVYDPKQDRKK
                     GIFTKLYEGILGGLSWILENRPREEVATTTKISGKLSDPETSVTDIILGLVQNAFFRA
                     ILPGLEREIEVGGRKGR"
     gene            complement(2493..3293)
                     /locus_tag="VF089_03185"
     CDS             complement(2493..3293)
                     /locus_tag="VF089_03185"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6172993.1"
                     /translation="MQSAPEERSNHVFYREVLDILRQAEIPFLISGGFALQQFTGILR
                     EVRDLDIFVLEKHIHAALEALARSGYPTELTFSHWLGKVYNHHRTEYVDLIFSSGNGL
                     CKVTESWFERAVPSEVFDTPVFLCGPEEMIWQKAFIMERERYDGADVAHLIRTQSDAI
                     DWERLIGLFGEHWRVLLSHVTLFEFIYPTERAKIPRWVKQRLIALLQREFDEPLTEEQ
                     VCQGPLLSRSQFRVDTEDWGYKDARLNVMSPEEVAQWTEAAEEKEEKP"
     gene            complement(3307..4062)
                     /locus_tag="VF089_03190"
     CDS             complement(3307..4062)
                     /locus_tag="VF089_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6172994.1"
                     /translation="MTDNMVHIAAIGDLHCKSTFAGKFRALFSQISENANILLLCGDL
                     VDYGLPEEAHVLVKELSDVTKRIPVIAVMGNHEFESGKIDEVQKILTDGGILLLDGEA
                     CEIDGIGFAGVKGFAGGFGERLLAPWGEAIMKKFVHEAVDEALKLESALAKLRTTHRI
                     ALLHYSPIAATVMGEPLEIYPFLGSSRLEEPLNRFSVTAAFHGHAHHGAPEGKTKTGV
                     PVYNVAMKLLEYSFPGQLPYRILNLPRVNGSPK"
BASE COUNT         1055 a         1061 c         1084 g          918 t
ORIGIN      
        1 tgtggaatac gagatcgtca aaggcaataa cacctacgaa gtgcagatcg atttcgataa
       61 gggcggcaaa gctagcgaga tcgatgtcgc ccctaatctc tggcgcaccg atgctaccaa
      121 agcagtgttg ctgggaagaa aggctgagac tcctaaagga gcggtagcca atcccgatcg
      181 ctatagcgat cgcgacagac gcaaggccta tgatagcgag tccgagaaac tggagaaagc
      241 actcaagacg ggagaaaaca aggagagcta tcgccggcaa atcgaaaaaa tgggttacaa
      301 aataacctcg gtaaacgccg acaagccgga ctacgtggaa tacgaggttg ttaaaggtga
      361 cgataccttc gaggtgcaga tcgactttaa ggatggcaaa gccaccaaag tcgatgttgc
      421 gccaaatatc tggcaggccg aagcgactga caaggctcta agccggagag aagaaaaagc
      481 tgagaagaga gaagagagag cggagaagcg ccaggaaaaa cggtgatgag tcaagctaca
      541 gtcaagagcc aactgcttta ggtcggcatg ccgtgggtgc gcgtcaggtg cgcgcaacca
      601 cggcatgaaa ccgagtagca ggtcagcatc ttggcaagct gcgagcagat agtctcaatc
      661 acaggtaaac agcggaggac aacacatgag cactcttctg atcatcattt taatactctt
      721 gcttatagga gctctcccaa cctggcccta cagcggcggt tggggatatt accctagcgg
      781 tggcctcggg ctcattctat taatcatcat tatcctcgtt ttaatgggcc gcctatgatc
      841 agttagcaca tcggcgacga ccgggtcgcg gcaacttttg atctatgaaa agggctttag
      901 tttggctgtt agctattttc gggttgcttg ttgtattgct ggtgatcgcg gccttcttca
      961 tcgacgagcc cctgcggcgc cggaccgaag cgaagatcaa cgcatcgctg aaaggctata
     1021 ctgttcggat cgagaagctc gatttccatc ctataggatt tagtcttgat ctcgaaaact
     1081 cggtgattac acaggatgcc aaccctgaac cggccgttgc cgaaataccg aatctaaccg
     1141 caagcgttaa ctggcgggct ttgctctccg gccacgtcgt ggcggatttc gaaataaata
     1201 accctaagtt gtttctcaac ttcaaacaat ccgaaaacga aatcaaagac gaaactccca
     1261 tcgaagagcg ggggtggcaa gatgccgtgc aggcgatcta cccgctcaag atcaatgaat
     1321 tcgtcatcac gggtggcgcg cttacctatg tagatcaagg ccagtaccgt ccgctggaac
     1381 tcacagatgt taaccttagg gcctataata ttcgtaacgt cagatctcaa gaaggcgaat
     1441 acccgtccga ggtgtatatc gaggggacgg tcttcaagaa tggcaaaatt gttctcgagg
     1501 ggaacgcgga ctttcttgcc gagccgcatg tcgccatcaa agcagcgatc caggcggagc
     1561 aaatcgaact cgattatttc aggcctatta cggaacgtta taatttttca gtccgcagcg
     1621 gagtgctgag caccaagggc agcatcgaat acgccaaaaa taaacgaatt ctcagtgtgc
     1681 cgacgctcaa cgttcaaaaa ctcgtggcgg actacgtgca taccaagccc gcgagttctc
     1741 cgacaaagga gctgtcgaag aaggcagata aggtcattcg agagcacagc aacgagccta
     1801 cgctggaatt ggacgtcaac gacgtaaaca tcactgacag tgagttgggg attgtcaata
     1861 aggcaagcaa gccccagtac cgtctgtttg tcactggcgc ccaggtcaaa attcaaaacc
     1921 tcagtaatca gggggaggat ggcaccgcga cgattacggc aaacggtaga tttatgaatt
     1981 cgggaaagac ccgtctgata gcgcgcctga gacccaaagg gaagagcccc aacttcgatt
     2041 tgcaattagc catcgatcag tcggagctca aagcgctcaa tcctttgctc agagcgtaca
     2101 tcggcgccga tgtggtcgcc ggacaatttt ctttctactc agagatcgca gttcgtaacg
     2161 gttcgatcaa cggctatgtg aaacctttgt tcaaagaaat cgacgtttac gatccgaaac
     2221 aggatcgtaa gaaaggcatc ttcaccaaac tctacgaagg cattttaggc gggctctcct
     2281 ggattctgga gaatcgcccg cgcgaggagg tcgctacgac gactaaaatt tccgggaaac
     2341 tttcagatcc ggaaaccagc gtgactgaca taatcctggg gcttgtgcag aacgctttct
     2401 tcagagcgat ccttcccggt ttggaacgag agatcgaagt tggcggcagg aaagggcgct
     2461 aaaaagtatt ggttaaacga tgacgctagt gattaaggct tttcttcttt ttcctccgca
     2521 gcttccgtcc attgcgctac ctcttcgggc gacatgacgt tcaaacgggc atctttatag
     2581 ccccaatcct cggtgtcgac gcgaaattgg gaacgggaaa gaagcggtcc ctggcatacc
     2641 tgctcctcgg taagcggttc atcgaactct cgttggagca gagcgatcag cctttgcttc
     2701 acccaacgag gaatctttgc gcgctcggtt ggatagatga actcaaacaa ggtcacatgg
     2761 ctcagaagca ctcgccaatg ttcgccaaat aatccgatta gccgttccca gtctatggca
     2821 tcgctttgcg tccgaatcag atgggcgaca tcggcgccgt catagcgttc ccgctccatg
     2881 atgaatgctt tctgccagat catttcttcc gggccgcata ggaagaccgg cgtgtcaaac
     2941 acttcactgg ggacggcgcg ttcgaaccag ctctcggtta ccttgcacag tccgtttccc
     3001 gagctaaata tcagatcgac gtactccgtc cgatggtggt tataaacttt gccgagccaa
     3061 tgtgagaatg tgagctcggt tggatagccg gagcgcgcaa gcgcctcgag cgccgcatgg
     3121 atgtgctttt cgagcacaaa gatatccaag tccctcactt ctctgaggat gccggtgaac
     3181 tgttgtagtg caaagccgcc gctgatcaaa aacggtattt ccgcctgtcg caggatatcc
     3241 aatacctcac ggtaaaagac gtggttcgat ctctcttcag gcgcgctttg cacagtgttc
     3301 aacctttcat ttgggcgagc cattgacccg gggtaagttc agaatgcggt acgggagttg
     3361 gcctggaaaa ctgtactcga ggagtttcat ggcgacgtta tacaccggga cgccggtttt
     3421 ggtctttcct tccggggcgc cgtgatgcgc gtgaccgtgg aacgcggccg ttaccgaaaa
     3481 acgatttaat ggctcctcca gccgactcga ccccagaaag ggataaattt ccagcggttc
     3541 tcccataact gtcgccgcaa tcggcgagta gtgcagtaaa gcgattcgat gggtcgtgcg
     3601 cagcttggcc agggccgatt caagctttag agcttcgtcc acagcctcat ggacgaattt
     3661 tttcattatg gcctcgcccc acggcgcaag cagccgttcg ccaaagccgc cggcgaaacc
     3721 tttgacccct gcgaacccaa tcccgtcgat ctcgcatgcc tcgccatcga gcaggagaat
     3781 gccgccatcg gttagtatct tttgcacttc gtcgatcttg ccggattcga actcgtgatt
     3841 ccccatcacg gcgataacgg gtatccgttt agtgacatcg cttaattctt ttaccagcac
     3901 gtgagcttct tcaggcaagc cgtaatcaac caaatcacca cacagcaata gtatgttggc
     3961 gttctcgctg atctgtgaaa aaagtgccct gaacttgcca gcgaaggtgc tcttacaatg
     4021 caggtcgccg atggcggcga tatgaaccat gttatcggtc atccatgcgc ccttcggtta
     4081 ttggatccca gctgggtatg aacctttgtc gcaacaaa
//
LOCUS       DASUTG010000141         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1504510, whole genome shotgun sequence.
ACCESSION   DASUTG010000141 DASUTG010000000
VERSION     DASUTG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504510"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1507)
                     /gene="thrC"
                     /locus_tag="VF089_03195"
     CDS             complement(161..1507)
                     /gene="thrC"
                     /locus_tag="VF089_03195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6172995.1"
                     /translation="MSYNAWFQCINGCPGQFSLLEIMYRCPLCGDLLEVRHDMDALRT
                     RAAAAWIKLFDDRYRQNLYPYGSGVWAKKEWVVPFIDNENIVSTFEGNTNLFWAERYG
                     KQLHLEDLWVKQCGNSHTGSFKDLGMTVLVSVVKQMMADGMPIDAVACASTGDTSAAL
                     AAYGAAAGIPTVVFLPRNKVSPAQLVQPLANGALVLSLDTDFDGCMAAVQQVTKHKHI
                     YLANSMNSLRIEGQKTIAIEIVQQCDWEVPDWVILPGGNLGNVSALGKGFLMMHELGL
                     ISKLPRICVAQAENANPFYRAFLTNFERFEPVTARETLASAIQIGNPVSIRKAITTLQ
                     RFDGVVEQASESELAEETARADRTGMFNCPHTGVALAALRKLVERKVIRSNERVVVIS
                     TANGLKFADQKIAYHAGTLVGVQPGHQNRPVELSADPKQIAAAISHYVERMRLLET"
     gene            complement(1956..2558)
                     /locus_tag="VF089_03200"
     CDS             complement(1956..2558)
                     /locus_tag="VF089_03200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6172996.1"
                     /translation="MPTKISGLSVGLAILVSILGLGWPENRRAVLHADDNDWESPHIA
                     PFAPTPPEVVSRMLELAEIKHGDVLYDLGSGDGRIVVAAAKRFGIRAVGFEIDPVLVK
                     DSRQIIKRAGLEELVEIREQDIRAVDFSPASVVTMYLYPAANLRLRPVLMRDLRPGSR
                     VISHDFNMGSWKPDRVERLQDRAGIPRTIYRWRIAEPAGS"
BASE COUNT          615 a          775 c          716 g          603 t
ORIGIN      
        1 agcgcattgc cgagcttcgg ccgcgcctca ccaccatgca ccgcgatagt accgatgcct
       61 cgggtcgctg cgccaggctt tgacgtcggt tgcacccccg tctgtgtctg cagcggcaag
      121 gtggcgggtt tgtcgctgtc cttaaatccg cgatcagcca ttaagtttct agtagccgca
      181 tgcgctcaac ataatgcgat atcgcagcgg cgatttgctt gggatcggcg cttaactcga
      241 cgggccgatt ctgatgtccc ggctggaccc cgactagagt gcccgcatga taggcaatct
      301 tctggtcggc gaacttaagg ccattggccg tcgaaataac caccacccgt tcattcgagc
      361 ggatcacctt gcgctcaacc agcttgcgta acgcggccaa ggcaacgccg gtgtgaggac
      421 agttgaacat tccggtgcgg tcggcgcgcg cggtttcttc ggcaagctcc gactccgaag
      481 cctgttcgac gacgccgtcg aagcgttgca gcgtcgtaat cgccttgcga atactgactg
      541 gattaccgat ctgaatcgcg ctggcgaggg tctctcgcgc ggtcacgggc tcgaaacgct
      601 cgaaattggt caggaaggca cgataaaagg gattcgcatt ctccgcctgc gcgacacaga
      661 tgcgcggcag cttagagatc aggccgagct catgcatcat taagaatcct ttgccgagcg
      721 cgctaacgtt gcctaggttc ccgcccggca agatcaccca atcgggcacc tcccagtcgc
      781 attgctgcac gatttcgatg gctatggtct tttgcccctc aatccgcagg ctgttcatcg
      841 aattagccag atagatgtgt ttgtgctttg taacctgctg gacggcagcc atgcaaccat
      901 cgaagtcggt gtccagcgaa agcaccaacg cgccgttggc cagcggttga accagttgcg
      961 cgggggaaac cttattacgc ggcaggaaaa ccactgtggg aatgcccgct gccgcgccgt
     1021 atgccgccaa agccgcggaa gtatcgccgg tggaggcgca ggcaactgcg tcgatcggca
     1081 ttccgtcggc catcatctgc ttcaccacgg aaaccagaac ggtcatgccg agatccttga
     1141 atgagccggt atgagagttg ccacactgtt tgacccagag atcttccaga tgcagctgct
     1201 tgccgtaccg ctccgcccaa aataaattgg tgttgccctc aaacgtcgag acgatgtttt
     1261 cgttgtcgat aaatgggacg acccattctt tttttgccca aacaccagag ccgtaagggt
     1321 acagattctg gcggtaacga tcatcaaaca gcttgatcca tgccgctgcc gctcttgttc
     1381 ttagggcgtc catatcatgc cgcacctcca gcaagtcgcc gcacaacgga cagcgataca
     1441 ttatttcaag gaggctaaac tggccgggac agccattgat gcactgaaac cacgcattgt
     1501 aactcatgat caagagcttt ctccgcttga gcggcaatcg aaacgatcaa ttccgatgcg
     1561 taagaacagt tatgcgagcc aactcaggtt cgagagaatc aagcagttat agtgagacca
     1621 accgtcggct ttgtgctaat gggaagttaa agagtagccg atagcattca atagtgtcaa
     1681 ctcgactctc tgagagcccc tgaataagtg ttcgaagata aagacagcag tcgtttggca
     1741 tgttaaccaa gtaaccgaca gcattcagct gacgagagac gcgggccgaa acggatgcta
     1801 gttttcgatg cgaaactgag tcaatgcttg atctagagat cgttcaactc ggagacctca
     1861 atcaccaggc attaccgcag gcgcagctgt gatgcctttg gcaggcgaaa aaacagtcga
     1921 gcgtgtatga tcgccgtctt cctgtcgaaa ttaggtcagc tccccgccgg ctcggcaatg
     1981 cgccaccggt agatggttcg ggggattccg gctctgtctt gcagccgctc cactcgatcc
     2041 ggcttccaac tgcccatatt gaaatcgtgg gatatcaccc tggaaccggg cctcaagtcg
     2101 cgcatcagca ccggcctgag acgcaggttg gctgcaggat agaggtacat ggtaaccacg
     2161 cttgcaggtg aaaaatcgac agctcggata tcctgctcgc gaatttcgac caactcttcg
     2221 agaccggctc gtttgatgat ttgccgcgaa tctttcacca acaccgggtc gatctcaaag
     2281 cccaccgctc taattccaaa ccgtttcgcc gcagctacaa cgattcttcc atcgcctgat
     2341 ccaagatcat aaaggacatc cccgtgttta atctccgcta gctccagcat tcgactcaca
     2401 acctcaggcg gcgtgggcgc gaagggagca atgtgtggcg actcccagtc gttatcgtcg
     2461 gcatggagta ccgcccgtcg attttccggc cagccaagcc caaggatgct aaccaggatg
     2521 gcgagcccga cactcaaacc agaaatcttt gttggcattt tacaggagct tcagtcggtc
     2581 cgtcgacgca gtatttacaa gatcatcttc gttgagtgga cctttttctc ccgtagtgac
     2641 aataaagcgg ggcttcggac tttttgttta tcatccctaa ataccgagtc gacctttcgg
     2701 agcatttcc
//
LOCUS       DASUTG010000142         6796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_170578, whole genome shotgun sequence.
ACCESSION   DASUTG010000142 DASUTG010000000
VERSION     DASUTG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170578"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1356)
                     /locus_tag="VF089_03205"
     CDS             complement(145..1356)
                     /locus_tag="VF089_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6172997.1"
                     /translation="MDETTKRDRFRWAILALISTSHIIGAAAQYGINTLAPFYKDELG
                     LSRAQVGLFFSAFYLAMTGASFGTGWLADRLGVRKTSLQGHLVLGVCTLTAALAPSFT
                     WAFASFFLAGFGYSFLNPASTKGVMSWFHRDERATAMGIKQTGVPAGGVVTAMLAPAL
                     VLLIGWRGALAALGMINFLFGFLFWSFWRDPTGDSVKLDSPERTSEDPAAPLKVWSLL
                     PVSCGTAIYLVGQMALITYTPLYLKDAMGFSPYWASQALALTQAGAMIGRIGWGVASD
                     RLFGGRRKIVLIVIGVMSALMILALSLMTPQSPTYLLLPVLFLAGVCLVGYQGVSYAL
                     IGEIAGKARTGAALGLMITINAGCATVGTPIFGYIVDRTGSYAIAWQVLGAFLTLGII
                     GLITLLKDPRD"
     gene            complement(1616..3211)
                     /locus_tag="VF089_03210"
     CDS             complement(1616..3211)
                     /locus_tag="VF089_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6172998.1"
                     /translation="MNNSDVIVGMLEKAGVRWVFGVPSGPVLPLIESLRRSSIEFILT
                     ASETSAGFMASVIGYLTGRPGICVSTVGPGATNLTTGVGCAWLDRAPVLAITCNVSSA
                     WLGRRIQMRIDHQALFKPITKWTVSFTDGNVSEILAKALAIALHEPPGPVHLDLPEDV
                     ALASGSEEWSESGEAKPGADCSESMAQALSQALSKSHRPLLVTGLTFARHNISHHLVQ
                     FIERQKIPFVSTLHGKGFLPESHSNWAGVIGRARRTDIKHFTDQADLIVAVGYDPIEM
                     NYEEWVGQTPVMHISTEAAEDDGSISFVWNQACPLNAAVAAMAKLPPVGNEWSVEDWR
                     DHRLSFDRALRPPSSQFAAYHALDILREKFAPNGILAYDVGAHTHQIASQWRTDCPKS
                     LLATNGWSSMGYGMPAAYAAKLIFPARQVVAVIGDGGFQMTAGELSLARRLRLQVPVI
                     VLNDGWLGLMKVKQERKHYHLSGVHLGPPPESPAHYFGVPCRPAKTPDEFSRALQWAM
                     TLDGPSIIEAFIDVEPYSQTVFD"
     gene            complement(3214..4014)
                     /locus_tag="VF089_03215"
     CDS             complement(3214..4014)
                     /locus_tag="VF089_03215"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX6172999.1"
                     /translation="MKSIGIVGCGAIGRALVAAAESGSLPVRVAGINSRTEQHAIEFL
                     ARFKSPPPYLSLPELINAADLLVEAAGASVVPGLAKEVFAAGKDLMVISVGALLNHPE
                     IIETSRRTGCRLYLPSGAIAGLDGIKSACVGPITHVTMTTRKPPDGLAGAPYLVQNQI
                     SLAGLTGEMEVFSGTAREACKGFPANVNVSAAVSLAGIGPDRTRIRILAVPGLKRNCH
                     DIEIEGEFGRLLMHIENVPSENPKTGKLTILSIIRALQDVVDPVRIGN"
     gene            complement(4145..4573)
                     /locus_tag="VF089_03220"
     CDS             complement(4145..4573)
                     /locus_tag="VF089_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX6173000.1"
                     /translation="MIEITSLPALNAALNSLSAVLLLTGYRFIRAGNKPAHRTSMLAA
                     CASSTLFLISYLFYHFHVGSVAFKGQGLIRPIYFTILITHTILATVVVPLAIITLLRG
                     LQENFIKHRRIARWTFPIWMYVSVTGVLVYWMLYWLVPSR"
     gene            complement(4570..4848)
                     /locus_tag="VF089_03225"
     CDS             complement(4570..4848)
                     /locus_tag="VF089_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6173001.1"
                     /translation="MATTTHTHPNYMGIFWVLLVLTVVEVGLTFLGLPKLLLGSLLVI
                     LAVWKAALVAMYFMHLKFEPKTLAMIAIVPFVLCLFMILMLLPDIRPV"
     gene            complement(4866..5474)
                     /locus_tag="VF089_03230"
     CDS             complement(4866..5474)
                     /locus_tag="VF089_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6173002.1"
                     /translation="MSEAAAAHPGVEPAESPLTPDSWGKLGMWNFLAGDAMSFGGLIV
                     GYGILRYASSNWPVPSEVLGINLTALMTFLLIVSSVTMVKALAAIQSGDKTSFKKFMG
                     LTILGGAIFLGLQAYEWTHLIHDTLPHYKLSFSSHLFATTFFVLTGFHGLHVTGGVIY
                     NTVVLALVSKGRWGANHVEIAGLYWHFVDLVWILIFTFVYLL"
     gene            complement(5471..5761)
                     /locus_tag="VF089_03235"
     CDS             complement(5471..5761)
                     /locus_tag="VF089_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173003.1"
                     /translation="MSALAKNILTAACLVLLAPELVRACAVCLTGAGDDSITEAFNLS
                     VLFLMAAPYAVVGGIVACLIYVQRGKAAQTKTEPDEEPAMPLATIQKESATR"
     gene            complement(5813..6475)
                     /locus_tag="VF089_03240"
     CDS             complement(5813..6475)
                     /locus_tag="VF089_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX6173004.1"
                     /translation="MARTSTARRLEEAPFAPANRLEFGGGDPPAPPVRPNTPIANNAW
                     LATVMFLGAEAMFFAGLIGAFIVFRMGSPIWPPPFQPRLPVGITGVNTIVLLASAITM
                     HMALRAVRAGAIDRAKRMLAVTAALGGVFLLIQGYEWARLIHFGLTVSSSIYGGLFYT
                     LIGFHAFHVAGALVWLIVVFVRANRGYLSRQRYIGLQVCAMYWTFVVALWPILYGLVY
                     LY"
     gene            complement(6539..>6796)
                     /locus_tag="VF089_03245"
     CDS             complement(6539..>6796)
                     /locus_tag="VF089_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173005.1"
                     /translation="FSRQWGSFGNGIEFDWRPPAIIRVIDLFAGAFVDDGLGPGAAMS
                     KMMNSIFSIREYRGKNRRLGSVLATALVLYIGAVIAFIIAY"
BASE COUNT         1743 a         1973 c         1785 g         1295 t
ORIGIN      
        1 aagaaaaaat tgaacgtagc gtcaaaagcg caaaaggcgc ctctcaattg ggaggcgcct
       61 tttttttggc aaacttcgac gttttcgtga cagctcccct gtatttccgg gtaagtccaa
      121 acccttgaat cgggtgaact atgattaatc acgcgggtcc ttgagcaacg ttatcagtcc
      181 gataatgccc aacgtaagaa acgcccccag cacctgccag gcaatcgcat acgatccggt
      241 gcgatcgacg atgtaaccga aaatcggcgt gccgacagtt gcgcagccgg cattgatcgt
      301 gatcatcagt cccaacgccg cgccggttct ggctttgccg gcaatttcac caataagcgc
      361 ataggaaact ccctgatacc cgaccaggca gacacctgct aaaaacaaca caggtagaag
      421 aagataagta ggcgattgcg gcgtcatcaa gctcaaggcg agaatcatga gtgcggacat
      481 tacgccgatg acgatcaaaa cgatcttgcg ccggccgcca aacaaccggt cgctggccac
      541 gccccaaccg atgcgtccga tcatggcgcc tgcttgggtt agcgccaaag cctggcttgc
      601 ccagtacggg gaaaagccca tcgcatcttt taaataaagc ggtgtatagg tgatcaaagc
      661 catttgtccg accaggtaga tagcggtacc gcagctgacg ggaagaagac tccagacctt
      721 gagcggcgct gccggatcct ccgaagtacg ctcaggagaa tcaagtttga ccgaatctcc
      781 cgtcgggtct cgccagaaag accagaacaa aaaaccgaat agaaaattga tcatgcccaa
      841 cgcagccagt gcaccgcgcc agccgatgag caacaccaat gcaggcgcaa gcatcgcggt
      901 aaccacaccg ccggccggta ctccggtttg cttgatgccc atcgctgtgg cccgttcgtc
      961 tcgatgaaac caggacatca cccctttggt ggatgcagga ttgagaaaac tgtagccaaa
     1021 cccggcaagg aaaaagctcg cgaaagccca ggtgaacgac ggcgcaagtg cggcggtaag
     1081 ggtgcacacg cctaggacca aatgcccctg caacgatgtc ttgcgcacac caagtcggtc
     1141 ggcgagccat cctgtgccaa aggacgcgcc ggtcatcgcc aggtaaaacg ccgagaaaaa
     1201 tagaccgact tgcgcgcgcg acaaacccaa ttcatctttg tagaaaggcg ctaaagtatt
     1261 gatgccgtat tgcgccgccg cgccgataat atgagaagtg ctgatcaaag cgagaatcgc
     1321 ccagcgaaag cgatcccgct ttgtggtttc atccatcgat ggagctgctt tcagcccggg
     1381 ttccgcagtt caaagtgaca cggcgatcaa acggcgcgcc ctccggagcc aaggcaatgg
     1441 gcctgcggct tgtagcggcc cgcaagctca acttgctgca gcgaactcct ggaaaaaata
     1501 tgagcgctaa accatgcacc ataaacaaaa gtgaaactcg tcgtttctgt gccttcgctc
     1561 ctccaggcgc accgcttgat cggcagcctt taatcgctac tgcggaattc tgagttcagt
     1621 cgaagactgt ctgagagtaa ggctcgacat cgataaaagc ttcgatgatg ctgggaccgt
     1681 ccagcgtcat cgcccattgc agcgccctac tgaattcatc aggagtttta gccggccggc
     1741 atggcacacc gaaataatgc gccggcgact ccggcggcgg accgagatga acgcccgaga
     1801 gatgatagtg cttgcgctcc tgcttcactt tcatgagacc cagccagccg tcgttcagaa
     1861 cgatcactgg cacttgcagg cgcaaccggc gcgccagaga tagctcgccc gcagtcatct
     1921 gaaatccgcc gtcgcctatc acagcgacga cttggcgggc agggaatatc agtttggccg
     1981 catacgccgc cggcatacca taacccatgg aggaccagcc attggtagcg agcaacgact
     2041 tcggacaatc cgtgcgccat tgactggcaa tttgatgcgt gtgcgcaccc acgtcgtagg
     2101 cgagaatgcc attcggcgcg aacttctcgc gcaaaatatc caacgcatga tacgcggcaa
     2161 actggctgct gggaggacgc agcgcgcgat cgaatgacag gcgatggtcg cgccaatcct
     2221 cgacagacca ctcgttgcca actggcggaa gctttgccat tgcggcaacc gccgcattga
     2281 gaggacaagc ctggttccag acaaacgaga tgctgccatc atcctcagct gcttcggtac
     2341 taatatgcat gaccggagtt tggccaaccc attcctcgta gttcatctcg atgggatcgt
     2401 aacccaccgc aacgatcagg tccgcctgat cggtgaaatg tttgatgtcg gttcggcgtg
     2461 cacggccgat gacgcccgcc caattcgaat ggctttccgg taagaagcct ttgccatgta
     2521 gcgtcgacac gaagggaatc ttttggcgtt cgataaactg cacaaggtga tgcgatatat
     2581 tatgacgggc gaaagtgagt ccggtaacca gtagcggccg gtgggattta ctgagcgcct
     2641 gcgaaagagc ttgagccatc gattcggaac aatccgcacc gggctttgct tcaccggatt
     2701 cactccactc ctcggatcca ctcgctaaag ccacgtcctc gggcaaatcc agatgaaccg
     2761 gtccgggcgg ttcgtggagg gcaatcgcca gagccttggc cagtatttcg ctgacgttgc
     2821 cgtctgtaaa agaaacagtc cacttggtaa tcggcttgaa taaggcttga tgatcgatgc
     2881 gcatctgaat gcggcggccg agccaggcgc tcgaaacatt gcatgttatc gccagcaccg
     2941 gcgcgcgatc cagccaggcg caacctacgc ccgtggtcag gttagtggct ccagggccga
     3001 cggtggaaac acagataccc ggacggccag tcaggtatcc aatcacggat gccatgaagc
     3061 cggccgacgt ttcgctcgcc gtcagaatga attcaatgga gcttcgccgc aacgattcaa
     3121 tcagcggaag gaccggaccg ctgggtacgc cgaaaaccca gcgcacgccc gctttctcca
     3181 gcatgccgac gatgacatcg ctgttattca tctctagttg ccgatgcgta ccggatctac
     3241 gacgtcctgc agcgcgcgaa taattgacaa gatcgttagc tttccggttt tcggattttc
     3301 gctaggaaca ttttcgatgt gcatcagcaa gcggccgaac tcgccttcga tctcgatgtc
     3361 atggcagttc cgcttgagcc ccgggacggc gaggatgcga attcgcgttc gatcgggacc
     3421 gatgccggcc aggctcaccg ccgccgaaac gttgacgttg gcaggaaaac ctttgcacgc
     3481 ctcccgcgcg gtgccggaga agacctccat ctctccggtt aatccggcaa gcgagatctg
     3541 gttttgcaca agatagggag cgcctgccag tccgtcaggc ggcttgcgcg tggtcatcgt
     3601 gacatgcgtg atcggcccca cacaagctga tttgatcccg tccaaacccg cgatcgctcc
     3661 ggatgggaga tatagccgac aacccgtgcg ccgggaggtc tcgataatct ccggatggtt
     3721 gagcagagcg ccaacgctga tcaccatgag atccttgccc gccgcgaaga cctcctttgc
     3781 cagtccgggc acgaccgagg cgccggctgc ttcgacgagc agatcggcag cgttgatcag
     3841 ctcgggcaac gataggtacg gtggagggct tttgaacctg gcgagaaact caatcgcatg
     3901 ctgctcggtt cgactgttaa ttccagcgac gcgcaccggc aaactgccgc tttctgccgc
     3961 ggcaaccaga gctcgaccga tggcgccaca accgacaatt ccaatacttt tcacgagacc
     4021 ctccacaatg ccggatcaac gattttgatc taaggctcta ctgaatttgg agttcgcgat
     4081 tctaaatgat gtcgctcatt tctactgcgg atcgcgcgct gaacacaacc tcctaaatca
     4141 gagatcaacg cgacgggacc agccagtaga gcatccaata cacgagcacg ccggtgaccg
     4201 aaacatacat ccatatgggg aaggtccagc gggcgatacg acgatgcttg atgaaatttt
     4261 cttgtaggcc tcgcagtaag gtgatgatcg ccagcggcac aaccacggtg gcgaggatgg
     4321 tgtgcgtaat cagaatcgta aagtagattg gccggatcaa gccttgaccc ttgaaagcca
     4381 cggaaccgac atgaaaatgg taaaacagat aggaaatcaa aaacagggtc gaggaagcgc
     4441 acgctgcgag catactggtg cggtgcgctg gtttattgcc agctctgata aaacgatagc
     4501 cggttaacaa tagaacggcg ctgagtgaat ttagcgccgc gtttagcgcc ggcaaggaag
     4561 tgatttcaat caaacgggac ggatatcggg cagcagcatc aaaatcatga acaggcagag
     4621 gacgaatggc acaatagcga tcattgccag agttttcggc tcaaacttca gatgcatgaa
     4681 atacatcgcc accagcgcgg ctttccatac cgcaaggatc accagcagag aaccgagcaa
     4741 caacttcggc agtcctaaaa acgtaagccc tacctccacc accgtgagca ccagaagcac
     4801 ccaaaaaatt cccatgtaat taggatgcgt atgcgtcgtc gtagccatgt ctttccccta
     4861 aaaacttaca gtaagtagac aaacgtgaag attagaatcc acaccagatc aacgaaatgc
     4921 cagtagagac cggcaatctc gacgtgattg gcgccccacc gccccttgct caccaatgcc
     4981 agcaccaccg tattatagat gacgccgccg gttacgtgca gtccgtggaa cccggttagc
     5041 acaaagaaag tggtggcaaa taagtgactc gagaacgaaa gcttatagtg cggcaaggta
     5101 tcgtgaatca agtgcgtcca ttcataggct tgaaggccga gaaaaatggc tccgccgaga
     5161 atcgtaagcc ccataaactt tttgaagctc gtcttgtcac ccgattgaat cgcagccaac
     5221 gcctttacca tggtcacgct gctgacgatg agcaggaaag tcatcaacgc tgtcaaatta
     5281 atgcccagaa cctcagatgg caccggccag ttggagctag cgtagcgtaa aatcccgtag
     5341 ccgacgatca acccaccgaa agacatcgcg tcaccggcga ggaaattcca catgccgagt
     5401 ttgccccagc tgtcgggagt caaaggcgac tcggcgggtt caacccccgg atgcgccgca
     5461 gcagcttcac tcatcttgtt gcactctcct tctgaatcgt tgctaacggc attgccggct
     5521 cttcgtcggg ttccgtttta gtttgagcgg ccttaccgcg ctgcacataa atcagacaag
     5581 cgacaatccc gccgacaacc gcatagggcg ccgccatcaa gaacaacaca ctcaaattga
     5641 acgcttccgt aatcgagtca tcacctgccc ccgtcaggca aaccgcgcaa gcacgcacca
     5701 actctggtgc caaaaggacg aggcaagcag ccgtcagaat attttttgcc aatgcactca
     5761 tacgtcaaac cgcagaagct aacggatcat gttttcgctg tcaacaaact catcagtaca
     5821 gatacaccag cccgtagaga atcggccaca gggcgacaac aaacgtccaa tacatggcgc
     5881 agacttgcaa gccgatgtac ctttgccgtg agaggtaacc ccggttggct cgcacgaaaa
     5941 ccacaatcaa ccagaccagc gcgccggcca catggaatgc gtgaaatcct atcaaggtat
     6001 aaaacagccc gccgtaaata ctgctcgaaa ccgtcagccc aaaatgaatc agccgagccc
     6061 attcgtatcc ctgaatcagc aagaacacac cgccgagcgc ggcagtcacc gccaacatac
     6121 gctttgcacg gtcgatggct ccggcacgca cagcacgaag tgccatatgc atggttatcg
     6181 cgctcgccag cagaacgatt gtattcacac cggtgatacc gaccggcaac ctgggttgaa
     6241 agggtggcgg ccatatcgga ctgcccattc gaaaaacgat gaaagcgcct atcagcccgg
     6301 caaagaacat cgcttcggcg ccgagaaaca tgacggttgc caaccatgca ttattggcaa
     6361 tcggcgtatt gggtcgcacc ggcggtgccg gcggatcacc gccgccaaac tccagccggt
     6421 ttgccggagc aaacggcgct tcctcgagcc gtcgtgccgt cgaagttcgt gccataattg
     6481 tcggatagaa cctataaaag agtcccgctc accaacagct cctcgccgat gtcgttcctc
     6541 aatatgcgat gataaaggcg atcaccgcgc cgatgtagag caccagcgcg gtcgctaaga
     6601 cggagccgag acgccggttc ttaccacgat attcccgtat ggaaaaaata ctgttcatca
     6661 tcttactcat agcggcaccc ggtccaaggc catcatcaac aaaagcaccg gcaaataaat
     6721 caatgacgcg tataatagcc ggcgggcgcc aatcaaactc gattccattg ccaaacgaac
     6781 cccactgacg agaaaa
//
LOCUS       DASUTG010000143         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2098267, whole genome shotgun sequence.
ACCESSION   DASUTG010000143 DASUTG010000000
VERSION     DASUTG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098267"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF089_03250"
     CDS             complement(<1..399)
                     /locus_tag="VF089_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6173006.1"
                     /translation="MAALYDLTIIGGGILGLATALKITAAHPRLSLLLLEKEPDLARH
                     QTGNNSGVIHSGLYYRPGSLKARTCVAGRKELIQFCDDNAVPYEICGKVVVAMTREEL
                     PRLEELHRRGEANGLRGLEIIGTERLKELEP"
     gene            complement(470..1405)
                     /locus_tag="VF089_03255"
     CDS             complement(470..1405)
                     /locus_tag="VF089_03255"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173007.1"
                     /translation="MAINIDWHSHHTAPEVSDRIKDLTGKRPHIDALDSPDFSKRIRE
                     MDEAGVDLQLICQGAGVYADQMPPEQALEIVRMSNRVLAERLVRYKDRLMGVTALSLK
                     NIPASVAEIERTAQQGFRAVLLYPHVEGKMLVDTPEMDPIFAKISELNLPIFLHGAGT
                     AQDPSLARLEDGGAGVAYAVLSDGEVAECCLRMIASGVFDRYPNLKIVIRSSGGGIPL
                     LLHRFFWKHKGPNGERRYSEIFLEHFLIDCASSDARTLSFLIDKMGENNMVFGSDYCG
                     GLDRLNRSFSAIMAQPNPDHVKGFMEKNSRQLLHI"
     gene            complement(1577..2188)
                     /locus_tag="VF089_03260"
     CDS             complement(1577..2188)
                     /locus_tag="VF089_03260"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173008.1"
                     /translation="MTKILHLLLLSLMFFGDPVTSMAGPPTDQIKATVDSAVGLLRDS
                     RDKPLGTNERRAQLREILFARFDFSEMARRALGAHWRRRTATEQQEFVQLFTELLERQ
                     YAGIIESYTDEKIVYVSERIDGAFAEINSKIFTAKGQELSINYKAQLVGQEWKVYDVI
                     AEEISLVNNFRSQFNRVISSSSYEELVRRLREKQADGVISVKN"
     gene            complement(2185..2646)
                     /locus_tag="VF089_03265"
     CDS             complement(2185..2646)
                     /locus_tag="VF089_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX6173009.1"
                     /translation="MNHSRTDIMVGIFVLAGAICLGYLAIRLGKVELFGATGYVVYAD
                     FSSVAGLKVGDPAEIAGVRIGKVESLGLVDDRARVTLRLDTNVKLQDDVIASVRARGL
                     IGDKFVLITPGASDNIIPPGGRIRETESPQDITDLIGKFVGGDLTSKKDQQ"
     gene            complement(2656..>3280)
                     /locus_tag="VF089_03270"
     CDS             complement(2656..>3280)
                     /locus_tag="VF089_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6173010.1"
                     /translation="VLLKHLNALLLPDRGEVLIDGIDITKLGQKELYQIRAKFGVLFQ
                     GAALLDSMTVFDNVGFPLREKTRLRESEIKTKVADRLEQVGLAEMGHKLPAELSGGMR
                     KRAGLARALIMDPEIVLFDEPTTGLDPVLATSIHQLISRTQNDFGYTGIIVSHTIPQV
                     FEISDYIVMLAGGIVEEVAPPQAFCVSENPIVQQFIHGRTEGPIDVL"
BASE COUNT          728 a          905 c          878 g          769 t
ORIGIN      
        1 tggctcaagc tcttttaagc gctccgtgcc gattatttcg agcccgcgca ggccgttcgc
       61 ttctccccgg cggtgcaatt cctccaaccg cggtagctct tcgcgggtca tggcaacgac
      121 gaccttgccg caaatttcat agggcacggc gttgtcgtcg cagaattgga tcagctcttt
      181 gcgtccggca acgcaggtgc gcgccttcaa cgagcccggc cggtagtaga ggcccgaatg
      241 gatcacacca ctgttattgc cggtctgatg gcgcgcgaga tcgggttctt tctccaacag
      301 caacagactc aggcggggat gcgcggcggt gattttcagc gcggtggcga gacccaggat
      361 gccaccgccg atgatcgtca ggtcatacag ggctgccatg aaatttcatt taaggtctag
      421 ccgtcagttc catgtcgagt tgactggccg ccggtctcgc gcgttcaagt taaatatgga
      481 gcagctgccg ggaattcttt tccatgaagc cttttacgtg atcgggattg ggctgcgcca
      541 ttatggcgct gaagcttctg ttcagccggt cgagcccgcc gcaataatcg gaaccgaaaa
      601 ccatgttgtt ttcgcccatt ttatctatca agaacgacag cgtccgtgca tcggagctgg
      661 cgcaatcgat gagaaaatgc tcaagaaaaa tttccgagta gcgccgctcg ccgttaggcc
      721 ctttatgttt ccagaaaaac cggtgaagca acagcggtat gccaccgccg gagctgcgga
      781 taacgatctt gagattggga tagcggtcaa atacgccgct ggcgatcatg cgcagacagc
      841 actccgcgac ttcgccatca gatagaaccg cataggcaac tcccgcgccg ccatcttcga
      901 gacgcgccaa ggatggatcc tgcgcagttc cggcgccgtg cagaaaaatc ggcagattga
      961 gctcggaaat cttcgcgaag atcgggtcca tctcaggcgt gtccaccagc atcttgccct
     1021 cgacatgcgg gtagagcagc acggcgcgaa agccttgttg cgccgtccgt tctatttccg
     1081 ccactgacgc agggatgttc tttagcgaca gcgccgtgac gcccattaat cgatctttgt
     1141 atcggacgag ccgttcggcc aacaccctgt tcgacatgcg tacgatctcg agcgcttgtt
     1201 ccggcggcat ctggtcggca taaacgcccg ccccctggca aatgagctgc agatcaactc
     1261 ccgcttcatc catttccctg atgcgcttgg agaaatccgg tgaatccaag gcatcgatat
     1321 gcgggcgctt accggttaaa tctttgatcc gatcggagac ttccggcgcc gtgtgatggg
     1381 aatgccaatc gatattgatt gccatctaga gctcccttcc agtcaagtct tataattgct
     1441 gcttcgaaaa aatagcccag ttagagcata ctggataggg tgattcggtg aaagaggcgc
     1501 gacgagcacg caagatgagc gccgggctgc cagcggcgct aaaaaacgaa tgaaaaatac
     1561 cagccggttc ggccggctag ttctttacag agattacgcc gtcggcctgc ttttccctaa
     1621 gccgtcggac cagttcctca taagaggaac tggagatcac gcggttaaac tgagagcgga
     1681 aattgttgac caggctgatt tcttcggcga tcacatcata gaccttccac tcctggccaa
     1741 ccaactgcgc cttgtagtta atcgacaact cctggccttt ggcagtaaat attttgctgt
     1801 ttatttcggc aaacgcgcca tcgattcttt cgctgacata gacgattttc tcatcggtgt
     1861 aggattctat aatgccggca tattgacgtt cgagcagttc ggtgaatagt tgcacgaact
     1921 cttgttgctc ggtggccgtg cgccggcgcc aatgagcgcc caatgcgcgc ctggccattt
     1981 cagaaaagtc gaagcgggca aaaagaatct ccctcagctg agcgcgacgc tcattggtgc
     2041 ccaacggttt atcccgggag tccctgagta gccccacagc gctatccacc gtcgccttga
     2101 tctgatcggt tggcggtccc gccatagagg ttaccgggtc gccaaaaaac atcaatgaga
     2161 gcaaaagaag atgcaaaatt ttcgtcattg ttggtctttt ttggatgtga ggtcgccgcc
     2221 gacaaacttc ccaatgagat ccgtgatgtc ttgcggcgat tcggtttctc tgatcctgcc
     2281 tccgggcggg atgatgttat ccgaagctcc tggtgtgatc aagacaaatt tgtcgccgat
     2341 aagaccgcgg gcgcgcaccg acgcgatgac gtcatcctgg agttttacat tcgtgtccag
     2401 ccgtaaagtg acgcgggctc ggtcgtcaac tagaccgagt gattctactt tgccgattct
     2461 gacaccggcg atttctgcgg ggtcaccgac tttcaagcct gccaccgaag agaagtcggc
     2521 gtagacaacg tagccggtgg cgccgaacaa ctctactttg cccaggcgta tggcgagata
     2581 gccgagacag atggctcctg cgaggacaaa aataccgacc ataatgtcag tgcgagaatg
     2641 gttcacttcg atctcctaca agacgtcgat gggcccttca gttctaccgt gaataaattg
     2701 ctggacgatt ggattttccg agacacaaaa agcttgggga ggggcgacct cttcaacgat
     2761 cccgccggca agcatcacaa tgtaatcgga aatctcgaag acttgcggaa tcgtgtgact
     2821 cacaataatc ccggtgtagc cgaaatcatt ttgcgtcctt gaaatgagtt ggtgaatgct
     2881 ggttgccaac accggatcca aaccggtggt aggctcgtca aagagaacta tttccggatc
     2941 cattatcagg gcgcgcgcca ggccggctcg ttttctcatt ccgccgctca gttctgctgg
     3001 tagcttatgt cccatttcgg ccaatccgac ttgctccagc cggtcggcga ctttggtctt
     3061 tatctcgctt tccctcaatc ttgttttctc gcgcaatgga aacccgacgt tgtcaaatac
     3121 ggtcatcgaa tcgagtagcg ccgcgccttg aaaaagcacg ccgaatttgg ctcggatctg
     3181 atacaactct ttttggccga gcttggtgat gtcgattcca tcgatgagaa cttcgccgcg
     3241 atccggcagc agcaatgcat tcaagtgctt caagagcact
//
LOCUS       DASUTG010000144         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_763799, whole genome shotgun sequence.
ACCESSION   DASUTG010000144 DASUTG010000000
VERSION     DASUTG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763799"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF089_03275"
     CDS             complement(<1..180)
                     /locus_tag="VF089_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6173011.1"
                     /translation="MMFTGSLVAIVTPFRKGKVDEQAFGDLIEWQIANGTNGIVPCGT
                     TGESATLTHEEHHRVV"
     gene            complement(215..1477)
                     /gene="lysA"
                     /locus_tag="VF089_03280"
     CDS             complement(215..1477)
                     /gene="lysA"
                     /locus_tag="VF089_03280"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6173012.1"
                     /translation="MHNFEYRQGELYCEQVPVSQIAKETGTPCYIYSHATLIRHIRAY
                     DSAFKSIPHLIAFAMKANSNLAILRLMAKEGSGVDIVSGGELFRAIKAGVPPSKIVFA
                     GVGKNAEEIRDALKADILMFNIESSAEMHALNDVAASIGRKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRALEEFRIASTLRHIEVVGVHAHIGSQLTEVTPFVESLKKVI
                     ALIEALKGQGTNIRYLNIGGGLGITYSDEKPPLPHELADAVSPLVRNLDLTLVMEPGR
                     VIVGNAGILVTKVLYEKIGEAKRFIIVDAAMNDLIRPSLYNAYHDIRPVSEALLHRPK
                     HVVDVVGPVCESGDFLAKDRTLPDVKPGDLLAVMSAGAYGFVMASNYNSRPRVPEVLV
                     KDGEVHVIRTRENYDDLIKGESIPAFLN"
     gene            complement(1470..>2489)
                     /locus_tag="VF089_03285"
     CDS             complement(1470..>2489)
                     /locus_tag="VF089_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173013.1"
                     /translation="DEEMVKLAAESGCVSVFVGMESLDEDCLDETNKPFNRVQKFSQE
                     IQMFHKYGIMVNPGIVFGFDNDDEAVFERAVDFLKKNQVELAYFNVLTPLPGTALFDR
                     YNNAGRIFDRDWSKYDGKHVVFQPSRMTPEQLQEGFYWANHQFYSLPNIWYRLSGTKQ
                     RFIARWEMNREFRKLVKRSCPKGRLSPLASVLKNLQAKLPVLDTDHLIPSALHALKQR
                     FDPKAPSTQQLTLNIKVKRHDKFAALFMDLEGAIDRLNVHELINRIREAAEKAKMDII
                     VNFENLKLAAPDALKALADSEAIKAAVPNVKVRYRKFRDAFETSMQELSLSGIEMLTE
                     DLQDA"
BASE COUNT          534 a          690 c          677 g          588 t
ORIGIN      
        1 cacgacgcgg tgatgttcct catgcgtcaa ggtggccgac tcgccggtcg tcccgcaggg
       61 cacgatgccg tttgtgccgt ttgcgatttg ccactcgatg agatcgccga atgcttgctc
      121 gtcgacctta cccttgcgga acggcgtcac aatcgcaaca agtgaacccg taaacatcat
      181 tcctcggcag acggaggttg aactcacacc gcgctcagtt taaaaacgct ggaatgcttt
      241 cccctttgat cagatcgtca taattttccc gggtacggat cacatggact tcaccgtctt
      301 tgaccaagac ctccggaact cggggccgag aattgtagtt ggaggccatg acaaagccat
      361 aggcgcctgc gctcatgaca gccagcaaat ctcccggctt gacgtccgga agcgtgcggt
      421 cttttgcgag gaagtcaccc gattcgcaca cgggccccac gacatcgacc acgtgcttgg
      481 ggcggtgcag taacgcttcg gagaccggac ggatgtcgtg ataagcattg tagaggctcg
      541 ggcgaataag gtcattcatg gctgcatcaa cgatgatgaa gcgcttcgct tcgccgattt
      601 tttcatacaa gaccttcgtc accaggatcc ccgcgttgcc gacgatgaca cggcccggct
      661 ccatgaccag ggtcagatcc aagttccgga cgagcggcga cacggcatcg gccaactcgt
      721 gaggcaaggg aggcttttcg tccgaatagg tgatccccaa tccgccgccg atgttgagat
      781 atcgaatgtt ggtcccttga cccttgaggg cttcgatgag cgcaatcacc ttcttcaagg
      841 attccacgaa gggggtgact tcggtcagtt gcgatccaat gtgtgcatgg acgcccacca
      901 cctcaatgtg ccggagagtg gaggcgatcc gaaattcttc cagggcacga tcagccgcga
      961 tgccgaactt gcttttcttc aggccggtgg aaatgtaggg atgcgttttc gggtcgatgt
     1021 ctggattgat tcgtaaggcc acccgggcct tcctgccgat cgaagcagcc acgtcattga
     1081 gggcgtgcat ttcggccgag gattcgatgt tgaacatcag aatatcggcc ttgagcgcat
     1141 cccggatttc ttccgcattc tttccgaccc ctgcaaacac aatttttgag ggtgggacgc
     1201 ccgccttgat ggcgcggaac aattcgccgc cggatacgat gtcgactccg ctgccttcct
     1261 tcgccatcag gcgtaaaatg gcgagattgg aattggcctt catggcaaac gcgatgaggt
     1321 gcgggatgct cttgaaggca ctgtcatacg cccggatatg cctgatcaat gtcgcatggc
     1381 tgtagatgta acagggcgtg ccggtctctt tggcgatctg gctgaccggc acctgctcac
     1441 aatacaactc gccctggcga tattcgaaat tatgcatcct gcagatcctc ggtcagcatt
     1501 tcgatgcccg aaagagacaa ctcctgcatt gaggtttcga aggcgtccct aaacttccgg
     1561 tatcggactt tcacgttcgg caccgcggct ttgatcgctt cggaatcggc gagcgccttg
     1621 agcgcatccg gggccgcgag cttcagattt tcgaagttga cgatgatgtc catcttggct
     1681 ttttcggccg cttccctgat ccgattgatg agctcatgaa cgttcagccg gtcgattgcg
     1741 ccctcgagat ccatgaacag agcggcaaac ttatcatgtc gtttgacctt gatgttgagc
     1801 gtcaactgtt gagtggacgg tgccttgggg tcgaaccgct gcttgagcgc atgcaaggcg
     1861 ctggggatca ggtgatcggt atccagtacg ggcagcttgg cctggagatt cttgagtacg
     1921 gaggcgagcg gagacaatct ccctttggga cagctgcgtt tcaccaactt gcggaattcc
     1981 cggttcattt cccatcgtgc gatgaaccgc tgtttcgtgc cggacagccg ataccaaatg
     2041 ttcggcagtg aataaaattg gtggttggcc cagtaaaatc cctcttgaag ttgctcagga
     2101 gtcatccggc tgggctgaaa cacgacgtgt ttgccgtcat actttgacca atcacggtcg
     2161 aaaatgcgac cggcattgtt ataccgatcg aacagtgccg tgcccggcaa cggagtcagc
     2221 acattaaagt aggcgagctc cacctggttt ttcttcagga aatcgacggc tcgttcaaat
     2281 accgcctcgt cgtcattgtc aaacccaaac acgataccgg gattcaccat gatgccgtat
     2341 ttatgaaaca tctgaatttc ctgggaaaac ttctgcaccc gattgaacgg tttattcgtc
     2401 tcatcaagac agtcttcgtc gagcgattcc attccaacaa acacagagac acaaccgctt
     2461 tccgcagcca gcttgaccat ctcttcgtc
//
LOCUS       DASUTG010000145         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1060477, whole genome shotgun sequence.
ACCESSION   DASUTG010000145 DASUTG010000000
VERSION     DASUTG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060477"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF089_03290"
     CDS             <1..231
                     /locus_tag="VF089_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173014.1"
                     /translation="GSVFILVRHTQDLWRKFRRLADALDHFTPALFFRTVTPVLVTIG
                     ILAVMAMVVAVIHERVSVFLLVMRSIDDWTSF"
     gene            complement(404..874)
                     /locus_tag="VF089_03295"
     CDS             complement(404..874)
                     /locus_tag="VF089_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173015.1"
                     /translation="MKKLNVLSSAGLIVALMNIPVASAQDDRRDDRRYDRRDDGRYDR
                     RDDRRDERRGNWWEGIIGGTTASSRDDRRDLEGTWYLNGDRDKKTQIIASRDGLEARN
                     ERGDSSQLEISRDGDVYARDWERGLRGNVRRDRIEWENGTTWTREPSGRNASRR"
     gene            complement(1108..1482)
                     /locus_tag="VF089_03300"
     CDS             complement(1108..1482)
                     /locus_tag="VF089_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173016.1"
                     /translation="MKIGFIGLLLALAIGIMNIPSTVSAQGYGWSSWRDNRNALQQLE
                     GTWYLNGDRSRRTEINASRRGLVARNERGQTTRLEISRSGDVYARDWQGGLRGDVRRG
                     RIDWENGTTWTREPSYRNARRR"
     gene            complement(1657..1926)
                     /locus_tag="VF089_03305"
     CDS             complement(1657..1926)
                     /locus_tag="VF089_03305"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX6173017.1"
                     /translation="MKRIVVSILFFALSLSPLTGWAASSSDNWRNLSPKEKDKVLRNY
                     QRWQNLPPQDKEHLREEWDRWQRLPQDRRERLKQRYEERQRRDKR"
     gene            complement(1982..2503)
                     /locus_tag="VF089_03310"
     CDS             complement(1982..2503)
                     /locus_tag="VF089_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6173018.1"
                     /translation="MLAKKLGTMCKEFEEDLVLYYYGEIGDYEKQKLEHHLGNCGGCA
                     RFIADLHRLLPQMVLSEELPQSFWDDYYRETVAKLTHQREQKFCWRNLFAPMRMWMIP
                     AFGTAVIAALALGVVLSKGNLSSLYNQAPERIPSEIVTDTEQLEFFRSLDMLEALSAL
                     EGMEVSKADPHAA"
     gene            complement(2518..3057)
                     /locus_tag="VF089_03315"
     CDS             complement(2518..3057)
                     /locus_tag="VF089_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6173019.1"
                     /translation="MALCQQGERKFFEILVRRYMEKAFRIALDFTRDTEEAKDLSQEA
                     FLRAFSRIKQFDGRSSFYTWFYRLVVNLCLDHLRRKGKVIWERLESERKDERERKELT
                     DNTSGPDQEAIAGETKRRVDKTLDAMPNKQRTAFILRNHEGLPIADIAKVMKTTEGTV
                     RVYLHRAVAALRQSLVELV"
     gene            complement(3414..4076)
                     /gene="npdG"
                     /locus_tag="VF089_03320"
     CDS             complement(3414..4076)
                     /gene="npdG"
                     /locus_tag="VF089_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6173020.1"
                     /translation="MENLSVAIVGGTGNLGGALALRLGAPGVRIIIGSRDAEKAKKAV
                     ETLKPSLRAGEIVGMTNQDSIRGADYVVIAVPYEGHAQMVQDIKGQVAGKIIIDTVVP
                     LNKGKPFVPTAGSALQEAQQILGDEAPVVGALHNISAVDLADVDSPLGDVLVCGDKAD
                     AKEKVMEIIRRIGARAFDGGPGSNAYVIEGLTGVIISLNRKYKSKHGSIRIVGIGEDP
                     GH"
BASE COUNT          903 a         1206 c          995 g         1041 t
ORIGIN      
        1 ggcagcgtct ttatacttgt gcggcataca caagacctgt ggagaaagtt tcgccggctt
       61 gccgacgctc ttgatcactt tacaccagcc ttgttttttc gaactgtgac tccggttctg
      121 gtgaccatcg gtattctggc ggttatggct atggtcgtcg cggtaataca cgagcgtgtc
      181 tccgttttcc tgttggttat gcgcagcatc gacgactgga cttcgttcta agtcatcagc
      241 acggagtgta tatgcgctgg ggttggccga gctacattta ccggatgaaa cctggagagg
      301 taagcgcctc tagcgataac ttaccgaaga gaacttggcg atggaacaaa gcagggagga
      361 ctcttattgt cctccctgct catacgtttc aatttaggtt tgattaacgt cgcgacgcat
      421 ttcttcctga cggttcccgt gtccaggttg ttccattctc ccactcgatt ctgtcgcgtc
      481 ggacgttgcc tcgtagacca cgctcccaat cgcgggcgta gacatcacca tcccgactaa
      541 tttccagctg actggagtca ccccgttcgt tcctagcctc gagcccatct cgagaagcaa
      601 tgatttgggt cttcttgtcg cggtccccat tcaagtacca ggtaccctcc aggtcgcgcc
      661 ggtcatctct cgagcttgct gtagtgccgc caataattcc ctcccaccag ttgcctcggc
      721 gctcatctcg ccgatcatct cggcggtcgt atctcccgtc atctcttcga tcgtaacgtc
      781 gatcatctct tctatcatcc tgcgcgctgg caactggaat attcatcaat gctacaatca
      841 aaccggccga cgatagtaca tttagttttt tcatgacgtt ttcctcctgc gaacattaga
      901 ttcgcaaccg tcatgccacg ctcgagaaac tcacctcttc gcgataatcg ttcttatctg
      961 ttgcgttcac gggacgaatt gaaggcgggt gaaccggcag agaaacacag tcatcagagt
     1021 agttgaagca aatctaaacg gttaaaaaga aagggccatc tcggcttctt ccgagatgac
     1081 actttctgca agcaaaacag atcaaagtta gcgtcgtcta gcattcctat agctgggttc
     1141 gcgggtccac gtagtgccat tttcccaatc gatgcgacca cgtcgaacgt cgccgcgcag
     1201 gccaccctgc caatcccgag cgtaaacatc accgctccgg ctaatttcta gacgcgtagt
     1261 ctgaccgcgt tcgtttcttg ctacgagccc gcggcgcgag gcgtttattt cggttctcct
     1321 agatcggtca ccgtttagat accatgtgcc ttccaattgc tgcagagcat tcctattgtc
     1381 gcgccacgaa gaccaaccgt atccctgcgc cgaaacggtg ctcggaatgt tcatgatccc
     1441 aatcgccaaa gctaagagta atccaatgaa accgatcttc atgcctgaaa cctcctctaa
     1501 gttagctttc aaacattctc cccggacccc cttctgatca ttcacagccg atctcttttt
     1561 ttagacgggg tgcctcgcaa ttggtttaca gccatatggg agctcaatta ggcacagcaa
     1621 tattagatga ttcttcggtg ctaattgact ctgagcctac cttttgtcgc gacgctgccg
     1681 ctcttcataa cgctgcttga gtctctcccg gcgatcctgt gggagacgct gccagcggtc
     1741 ccactcctca cgcaagtgct ctttgtcttg aggcggaaga ttctgccagc gctgatagtt
     1801 gcgtaagact ttgtctttct ctttgggact taagttgcgc cagttatcgc tgctactggc
     1861 tgcccagccg gtcagtggcg acagcgacaa tgcgaagaaa agtatcgaaa caacaatgcg
     1921 tttcatctcg atgatctgct ctcttttgtt attacctgtt cagcaatcgt cgccagcctg
     1981 ctcaagcggc atggggatcg gccttgctga cttccatgcc ttcaagagcg ctcagagctt
     2041 ccagcatgtc gagcgatcta aagaactcca actgttcggt gtcggttacg atttcactag
     2101 gaattctttc tggcgcttgg ttgtagagcg aacttaagtt tcccttgctt aaaaccacac
     2161 cgagagccag agcggcgata accgccgtgc cgaacgccgg aatcatccac atgcgcatcg
     2221 gtgcaaaaag gttgcgccag cagaactttt gctcccgctg atgcgtcaac ttggcgaccg
     2281 tctcccgata gtaatcatcc caaaaagact gcggaagttc ttcggacagc accatctgcg
     2341 gcagcaatcg atgtaaatcc gcgatgaagc gagcgcagcc gccgcaattg ccgagatggt
     2401 gttccagctt ttgcttttcg taatcaccga tctcgccgta gtagtagagc accaaatctt
     2461 cttcgaactc tttgcacatc gttcccagct tttttgcgag catggtcatt tgcctcccta
     2521 aacaagctca accagagact ggcgcagcgc cgctaccgcc cgatgcaggt acacccgaac
     2581 cgttccttcg gtagtcttca tgactttggc gatatccgca atcgggagtc cttcgtggtt
     2641 tcgcaagata aaagccgtgc gctgcttgtt tggcatggca tccaaagtct tgtcgacacg
     2701 ccgtttggtc tcgccggcaa tcgcttcctg gtccgggcct gacgtattat ccgtcagttc
     2761 cttccgctcc cgttcgtctt tccgttcact ttcgagccgt tcccagatga cctttccttt
     2821 ccggcgaaga tgatccagac aaagattaac caccaaacga tagaaccacg tgtaaaaact
     2881 cgaacggccg tcgaactgct tgatgcgtga gaaggctcgc aaaaaagctt cctgggagag
     2941 atctttcgcc tcctccgtat cccgcgtaaa atccagggcg atccggaaag ccttctccat
     3001 ataacgccgg acgagtatct caaaaaactt cctctcgccc tgctggcaca atgccacaag
     3061 ctccaaatcg ggaagatctt ccggcgaacg attttcaggc gttgcgtgaa ttgatttacc
     3121 ctgcctctgc tcgtgttctg agggccccta gagttttaga cgtagagact tgagaaaggt
     3181 taaccgcgct ggtgcggcgg ctaaaacaag caagggcaaa gcactgggtc agtacctgaa
     3241 tcggcgttgt gggggagttg agcgatcgcc aactagggaa acatacgatg acgggtgctg
     3301 cgaccgctag tgttttggtg ccaaggccga tgatccgaaa agaactcaga attgcacaat
     3361 catattagac cgatctatct cagccgatca taggcggcgc taagttcgtc aggctaatgc
     3421 cccggatcct caccaatacc gacgatcctg atactgccat gcttggattt gtacttccga
     3481 ttgagagaga tgatgacgcc ggttagcccc tcgatgacgt aggcgttgct cccgggaccg
     3541 ccgtcgaacg cacgcgcgcc gatacgccgg ataatctcca ttactttctc cttggcgtca
     3601 gctttatcgc cgcagaccaa cacatcgccg agcggggaat cgacatcggc caaatcaacg
     3661 gctgatatgt tatgcagcgc gccgacgacc ggcgcctcat cgcccagaat ctgctgtgcc
     3721 tcctgtaatg ccgaaccggc tgtcggcacg aacggcttac ccttgtttag cggcacaacg
     3781 gtatcaatga tgatctttcc agccacctgt cctttgatgt cctggaccat ttgagcatgg
     3841 ccttcgtaag gcacggcgat gaccacgtaa tcggcgccgc gaatcgaatc ctggttcgtc
     3901 atgccgacta tctccccggc gcgaagactc ggttttaaag tttccaccgc cttcttggct
     3961 ttctccgcat cacgcgaacc gataatgatc ctcaccccgg gggcccctaa acgaagcgcc
     4021 agcgcgccgc cgagatttcc cgttccgcca acaattgcca cgctaagatt ttccatgatg
     4081 ttgaatcatc gaaaaagatc ctccgcttgc ggacgcagga gatccttcac cgatccttcc
     4141 tcctt
//
LOCUS       DASUTG010000146         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1950189, whole genome shotgun sequence.
ACCESSION   DASUTG010000146 DASUTG010000000
VERSION     DASUTG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950189"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF089_03325"
     CDS             <1..705
                     /locus_tag="VF089_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6173021.1"
                     /translation="TTSRGLTNKRPMAEYVLASFLHFSRGLHRSYRDKRCHRFEHQTY
                     NPVMLRDKTACVVGAGGIGQEVGKLCVAAGMRVVGTRGHVDPGATPPAGFTRLESAGA
                     LHDLLRESEFVAICCPWTQATTHLIDREMFAAMKTGTVLVNIARGEIIDEQALIEALA
                     QGKLRGVALDVYDGEFERAPDARLWDDERVIITPHVSAASDVSEHGGVNLFCDNLIRF
                     IEGRPLENVIDWKRGY"
     gene            complement(762..1784)
                     /locus_tag="VF089_03330"
     CDS             complement(762..1784)
                     /locus_tag="VF089_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173022.1"
                     /translation="MKKLVLLFCLLWASEVSAQSGFYQGKTIMVMAGVSAGSVYDLYT
                     RLLTAHMGKHIPGQPAFVVQNMTGAGSIIGANHLYNISKPDGLTIGAVQPTIFFNQLL
                     KQPEVRFDWAKFGWIGSLDKSDWLLYLRSDLPYKSLADVRQAAVPPKCGSTGASTSES
                     YFPKLLEQTIGTKFTVVAGYKGGGEIDLAVERGELQCRAFTIQAFHSREPYHTWRKNN
                     FVRIVMQTGRTRDPRLPEAPTFQELMDEFKTPDAARRLVPLVMAGGEFGRPIIAPPGV
                     APDRMRILRAAFMTTVKDPEFIAEAKSKNLDITPTRGEDLEALAKDVVTQPPEMVERL
                     KKLMSQ"
     gene            complement(2049..>2784)
                     /locus_tag="VF089_03335"
     CDS             complement(2049..>2784)
                     /locus_tag="VF089_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173023.1"
                     /translation="VLGGASQALLAVTFVGTLWAFVAHERKTPSPMIDLSFFSVPAFG
                     YGSVGLLMCCITQGLVTFVAPFYLQEVLKLSPTFMGIIFLAPSLLSMVLSPVSGVLTD
                     RIGARFLLMSGVVILMAAFLLGANLGADSHWIWPAVLLALTGIGSAFFNTPSQAVMIS
                     SLPKEHWGTAIGIINGIFGLGHMLGISLSGIFLTLAFRYYSGIPGTTPDPGDTQVFVA
                     SMNVTFMFALAISVVPLLTSMKLKKK"
BASE COUNT          654 a          791 c          786 g          553 t
ORIGIN      
        1 accacctcac gcgggctgac caacaagcgg ccaatggccg agtatgtgtt ggccagcttc
       61 ctccatttct cgcgcggcct tcatcggagc taccgggata agcgctgcca ccgcttcgag
      121 catcagacct acaatcccgt aatgctccgc gataagacgg catgcgttgt cggagcgggc
      181 ggcatcggcc aggaagtggg aaagctctgc gtagcggctg ggatgcgcgt cgtcggaacg
      241 cgaggtcacg tcgatcccgg tgcgacgccg ccggcgggct ttacgcgcct ggaatccgcc
      301 ggcgctctgc acgatctcct gcgcgagagc gaatttgtcg ccatctgttg cccatggacg
      361 caagcaacca cgcacctgat cgatcgagag atgtttgcag cgatgaaaac cgggaccgtt
      421 ctagtcaaca tagcccgcgg cgagatcatc gacgaacagg cactgatcga ggcattggcc
      481 caaggtaagc ttcgcggcgt cgctctcgac gtttacgacg gtgaattcga gcgagcaccc
      541 gatgcgcgcc tgtgggacga tgaacgcgtg atcattacgc cccatgtttc ggcggcaagt
      601 gacgtgtccg agcatggcgg cgtgaacttg ttttgcgaca acctgatccg ctttatcgaa
      661 ggccggccgt tggaaaatgt catcgactgg aagcgcggat attgaagaga gccgcggcct
      721 aggctcacgc ctgcgcggtt tcattgcaac gtcggatgcg gttactggct catcaatttc
      781 ttcaatcgtt cgaccatctc cggcggttgc gtaacgacat ctttcgccag agcctccaga
      841 tcttcgccgc gtgtcggagt gatatccaga tttttgcttt ttgcctctgc gataaattca
      901 ggatccttca ccgtcgtcat aaaagcagcg cgcaaaattc tcatcctgtc cggcgcgact
      961 cctggcggag ctatgatcgg ccgcccgaat tccccacccg ccataaccaa gggcactagc
     1021 cggcgcgcgg cgtcgggagt tttgaattca tccataagct cttggaatgt aggcgcctcg
     1081 ggcagacgcg gatctcgagt ccgtccggtc tgcatcacga ttcgcacaaa gttgtttttg
     1141 cgccaggtat gataaggctc acgcgagtga aaagcctgaa tagtaaaggc ccggcattga
     1201 agctcgccgc gctccaccgc cagatcgatc tcaccgccgc ctttgtatcc ggccacgaca
     1261 gtaaacttgg tgccgatggt ttgttccagg agcttgggga agtaactttc ggatgtgctg
     1321 gctccggtgg agccgcattt cggcggcacg gctgcttgcc gcacgtcggc aaggctctta
     1381 tagggtagat cggaacgcag atagagcagc cagtccgatt tatcgaggct gccgatccaa
     1441 ccgaatttcg cccaatcgaa tctaacctct ggctgcttca agagctgatt gaaaaagatc
     1501 gtcggttgca cggccccaat cgtcagtcca tcgggtttcg agatgttata aagatgattg
     1561 gcgccgatga tcgatccggc cccggtcatg ttctgcacga cgaaagctgg ctgtcctgga
     1621 atatgtttgc ccatatgcgc cgtcagcagg cgcgtgtaca gatcgtagac gctcccggcg
     1681 ctgacaccgg ccataaccat aatcgttttt ccctggtaga agccgctctg cgcgctcact
     1741 tcagacgccc acaaaaggca aaacaagagg acgagtttct tcatacaaac ggctgtaaca
     1801 cgccggctgc aggccaagca acggaaaagt gagtcgatgg tcgcagcgca agcctacagg
     1861 ttctctttgc gatgactgct ctcccgggac gatacagcag ttcggtggaa actccgaaat
     1921 gcaggctgat caaaacatct caaatacaag gcgcgcgaag aaccggagag ctgaggcgta
     1981 cgtcggcagt acattgaagc gagacgaacc agcgcaacga ccgcatacga ggttttttca
     2041 atagcctgct acttcttttt tagtttcatc gaagtcagca atggaacaac gctaatcgcc
     2101 agtgcaaaca taaaagtcac attcattgaa gcaacaaaga cttgcgtgtc gccgggatca
     2161 ggcgtcgtcc ctggaatgcc tgaatagtat cggaaggcca gggtaagaaa tattccgcta
     2221 agggatatgc cgagcatatg tcccagcccg aagattccgt tgatgatgcc gatcgcggtg
     2281 ccccaatgct ctttaggcaa cgagctaatc atcactgcct gcgacggcgt attaaaaaaa
     2341 gccgagccga ttcccgtcag cgctagcagc accgcgggcc agatccagtg agagtcggcg
     2401 ccgagattag cgccgagtag aaaggcagcc atcaggatca ccactccgct catcaggaga
     2461 aaacgggcgc cgatacgatc cgtcagaacg ccgctcacgg gcgacaacac catgctaagg
     2521 agcgacgggg ccaaaaagat aattcccata aaggtcgggg aaagcttgag tacctcctgg
     2581 agatagaaag gcgcgacaaa cgtaaccagc ccctgagtta tgcagcacat gagcaagccg
     2641 actgagccgt agccgaaggc aggcacggag aaaaaagaca ggtcgatcat cgggcttggg
     2701 gtttttcgct catgcgcgac gaaagcccag agcgtgccga cgaacgtcac agccaaaagc
     2761 gcctggctgg cgccgcccaa cact
//
LOCUS       DASUTG010000147         9691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_467695, whole genome shotgun sequence.
ACCESSION   DASUTG010000147 DASUTG010000000
VERSION     DASUTG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467695"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VF089_03340"
     CDS             <1..727
                     /locus_tag="VF089_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173024.1"
                     /translation="VRLNVDTIVTGGTPQLLAAKHATRTIPVVMAFAGDPVEQGFVVS
                     LARPAGNITGLSAVAPDLSGKRFELLKETVPTLSRAGVLWNPADPGIRRNFNETETAA
                     RALGVQIHSLEVWSPNQFGSAFKAATGARVQAVNVLQAHLTNEHRRQIVKLAAQSRLP
                     TMFGEEGLMDAGGLMSYGPDYGDLFRRAALYVDKILKGAKPADLPVEQPTQFELVINL
                     KTAQQIGLTIPPHVLARADRVIR"
     gene            1068..2054
                     /locus_tag="VF089_03345"
     CDS             1068..2054
                     /locus_tag="VF089_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173025.1"
                     /translation="MIRRIVVCLLITALLPSLYFAEAQQSKKFWRIGFLSSVSPEPSA
                     HLFNAFLQGLADLGYIEGQNVAIEPRWAEGKTERLSELAAELVRMKVDIIVSTGGTVT
                     AFAAKSATPSIPIVFTVGGDLVALGLIASLARPGGNLTGLSLLTTELNVKRLELMKET
                     FPKVRRVGLIGNPANPNYPIQLQETQAAAKQLELQVQSLEARNRNAIESVFSTVAEKS
                     LGGLLVLSDPMLNAHRERIAALAIKSRLPAIYEFKEFVEAGGLMSYGTNIVAVYRRLG
                     IYIDRIFRGAKPAELPVEQPTSFEFVINLKTAKQIGVTVPPNVLARADSVIK"
     gene            2104..3081
                     /locus_tag="VF089_03350"
     CDS             2104..3081
                     /locus_tag="VF089_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173026.1"
                     /translation="MIRKIAICLLTIALLPTVYFAEAQQSKQIYRIAILSPATRPRPV
                     VDALRQRLRELGYTENQNVVFEDRYAEFKVERLPELAAELVKLKPDVIFTHSITGARA
                     AKQATSIIPIVIGAAGDLVKDGIVSSLARPGGNVTGVTLLSSELEGKRLELLKESNRK
                     LSHVAVLVNSANSAWENYPQVLEPAGQALKVRLARAEVRQSADLEGAFAAMRRNGVDG
                     VLVVSDPIFQTYQRRIAEFAARQQLPSISEVPGFAEAGGLIQYGLSIPEMGRQAAVYV
                     DKILNGANPADLPIERPTKFEFLINLKSAKQLGLILPPAMLARADKVIK"
     gene            3115..5517
                     /locus_tag="VF089_03355"
     CDS             3115..5517
                     /locus_tag="VF089_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6173027.1"
                     /translation="MKVRDLPILKSFGVRRRRERLLRDYFLISVLLIGGGLITSGLVE
                     IYYRYQESQEQLALLQKEIAAGAAFKIEQFVQEIHNILKGATKSRDIAPKGLTPEFRF
                     ELEKLLIIAPAVTEALALDENGTIQVQASRLRTVLPDAKKDLSASPSFRQARQGKSYF
                     GQVYFVRGSEPYMTIAVPIERFAGDVIGVLQAEVNLKYIGDVISNIRVGKAGHAYTIS
                     RSGELIAHPDISLVLQRRNVLHLEQARAAFRPTTPGGPASNAIVATDLQGKDVLSSFA
                     LIPILDWAVVLERPVEEAYEALYASMIRTSTLFMIGLGMALIASLFLARRVVRPVRIL
                     RQGVERIGSGDLAYRLDLKTGDEMEILADEFNKMTSQLQESYANLEQKVEARTKELTE
                     SLEQQTATSQVLGVISSSPRDLQPVFETMLANATRLGSSDYGAIFRFDGKIFSLVTAQ
                     YVSPHMTVLADRTFPPDPTTAVGRLGLEKKIVHIPDVSMDPTWNPPEEYRKEGIRSVL
                     AVPMLKENNLVGAIVVYRREIQPFTESQIALLKTFADQAVIAIENVRLFKELEEKNEQ
                     LEAANRHKSQFLANVSHELRTPLNSIIGFTRLVLRKIEGQIEKLQKENLQKVLISAEH
                     LLKLINGLLDLAKIEAGRMEVYAETFKIDDVVRVATATVEPMLRNGNVRLVTEMPPEL
                     PAIKTDRDKLKQVILNLLSNSAKFTEAGEIKVAAWSENGSLKLAISDTGIGMNKDALH
                     YIFDEFRQVDMSTTRKYGGTGLGLTIVKRLIDLLGGEIGVESEVGKGSKFTITLPLNH
                     NN"
     gene            5624..5992
                     /locus_tag="VF089_03360"
     CDS             5624..5992
                     /locus_tag="VF089_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173028.1"
                     /translation="MGTKKKILVVDDTEFNRDLVVQLLEDEFELVIAEDGAEGLKQTQ
                     SERPDLILMDLGMPIMDGWEATRKIKANDELKHIPIIAVTSHAMAGDEVEARRAGCDD
                     YLPKPIDEDELLKKIRKFLF"
     gene            6052..7137
                     /locus_tag="VF089_03365"
     CDS             6052..7137
                     /locus_tag="VF089_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173029.1"
                     /translation="MPNKILIVDDEPFNLDLLEQELQEQGYEIERASDGAEALEKLPV
                     FVPDVVLLDYMMPRMNGLEVLKRLRADEQWRSLPVILLTAKAAQEDKITGLDAGADDY
                     VIKPFDAFELLARVRSMLRIKELHDSLEELNKTLSQKVEQQVGEIERMKRLKRYLSPQ
                     IADTLIASNDESLFKTHRREITVVFLDLRGFTAFSDSAEPEEVMELLRTYHAAMGKLV
                     FKYEGTLERFAGDGIMIFFNDPIPCEDHTERAIRMATEMRSRAKELRSGWVKKGYDLD
                     LGIGLASSYATLGNIGFEGRMDYGAVGNVTNLAARLCDEAKGGQILTNQRTLSKVEDL
                     IEFEACEELHLKGFSRPVAAFNIIKLK"
     gene            7268..8614
                     /locus_tag="VF089_03370"
     CDS             7268..8614
                     /locus_tag="VF089_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6173030.1"
                     /translation="MAHRILLVDDEPFNLDLLEQELADQGHSIATCASGEEALEKLAG
                     FQPDLILLDYMMPGMNGLEVLKEIRRKGLDTPVVIVTAHGTIERAVETMKEGAEDFLT
                     KPFDPDHLAIVVKKALERTRLREEVEILSEQLGARFKMVQGRSLSMARAVIEAQKAAV
                     SKSTVLLLGESGTGKEIFARAIHNWSQRKSAPFIPINCVGLSKDLLESELFGHEKGAF
                     TGAHQLKKGRVELADGGTIFLDEVGDISPELQAKLLRFLQEREFERVGGTHQISVDVR
                     IVAATNRNIDKAVSEGTFREDLYHRLNVIPITLTPLRERREDITELAAHFLQRFSVET
                     KKRFSGISEDAEATLMTYHWPGNVRELANVIERAVVLGDGPVLTIDLLPARIAGLKPT
                     NITAVTKSYHETINHHRRDLILKALSEAGGNRAAAAKALGLHRTHLLKLIKALQLG"
     gene            9483..>9691
                     /locus_tag="VF089_03375"
     CDS             9483..>9691
                     /locus_tag="VF089_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6173031.1"
                     /translation="MLLLIIPTLLSTASRYAWGQSKINYKVIPNLEAMKDQAPTPDFT
                     LPNFDSKKISLKDFRGKLVFLNFWAT"
BASE COUNT         2425 a         2484 c         2630 g         2152 t
ORIGIN      
        1 ggtccgcctt aacgtggaca caatcgtgac tggcggtacg ccccagcttc ttgctgctaa
       61 gcacgcgacg cggacgattc ctgtcgtcat ggcatttgcc ggcgatcctg tcgagcaggg
      121 ctttgtcgtc agtcttgctc ggccggcggg taacatcacg ggcttgtcgg cggtcgctcc
      181 tgatctaagt ggaaaacggt tcgaactgct aaaggaaacc gttccgacgc tcagccgtgc
      241 aggagtcctg tggaatccag ccgacccggg tattaggcga aatttcaacg agacagaaac
      301 tgcagcgcgg gccctcggtg tgcaaattca ctccctggag gtatggagcc ctaaccaatt
      361 tgggagcgcg ttcaaagctg ccaccggggc acgggtgcag gcagtcaacg tgctccaggc
      421 tcaccttaca aatgagcatc gaagacaaat tgtaaaactt gcggcacaga gccgacttcc
      481 gacgatgttt ggagaggaag ggctaatgga cgctggcggg ctcatgtctt atggaccgga
      541 ttatggtgat ttattccgcc gcgccgcgct ctacgtagat aaaatcctca agggtgcgaa
      601 gccggcggat ctcccggtag agcagccaac ccagttcgag cttgtaatca atctcaagac
      661 agcccaacaa atcggtctca caattccgcc gcacgtgctg gcacgggcgg atcgggtgat
      721 tcgatgatag caagcagcga gcacacggca gaaagcagca agaaaatggc agcaaagccg
      781 cattaataag gattttgtct gcttcctgct taccgtctct ctgcttacat tttcgatcgg
      841 agtctgcaca gccgcgtcgc cgtgatggcc gataaaattg taaaaggcgc gaagccgccg
      901 atttactatc gacggaccga gagtgtttga tctggtaatc aatccgaaaa tcacgaagca
      961 gatcggcgta accattccgc aaagggtcct gggcgaaggc ggacagagtg atccgtgaca
     1021 gaaccagaaa gcagaaagtt tcagcagcgc gcacgagagt aaaaccgatg attagacgga
     1081 ttgttgtgtg cttactgatc actgctttac tgccaagtct ttatttcgcg gaggcgcagc
     1141 agtccaagaa attctggcgg atcggtttct tatcgtcggt gtcgcctgag ccgtctgcac
     1201 atttatttaa cgcattcctt cagggcctcg cggatctcgg ctatatcgag gggcaaaacg
     1261 tcgcgattga gccgcgttgg gcggagggta aaacggagcg tctatcggag ttggcggcgg
     1321 agttggttcg tatgaaagtc gacatcatcg tttctaccgg gggcacggtc acggcctttg
     1381 cggcaaaaag cgcgacaccg agcatcccga tagttttcac ggtcggtggt gacctcgtcg
     1441 cactcggcct tattgccagc ctggcgcggc ctggcggcaa tctcaccgga ttgagtctcc
     1501 tcacgacgga gctgaacgta aaacggctcg agttaatgaa agaaacattt ccgaaagttc
     1561 gtcgcgtggg gcttatcgga aatcccgcca atccaaatta tccgattcag ttgcaggaaa
     1621 cgcaagccgc cgccaagcag ttagaattgc aggttcagtc tctggaagca cgaaaccgga
     1681 atgctattga gtctgtgttc tcgacggtgg cagaaaagag cctcggtggg ctgcttgtgc
     1741 tgtctgaccc aatgctcaat gcgcacagag aacggatcgc cgctctcgca atcaaaagcc
     1801 gcctgcccgc gatttacgaa ttcaaggagt ttgttgaagc tggaggtctc atgagctacg
     1861 gaacaaatat tgttgctgta taccgccgct taggaatcta tatagacaga atcttcagag
     1921 gggctaagcc agcagagctg ccggtcgaac aaccgactag tttcgaattt gtcatcaatc
     1981 tcaaaaccgc caagcagatc ggcgtaaccg ttccgcctaa cgtgctggcg cgggcggata
     2041 gtgtgatcaa gtgacatcag gtggtgagca gaaagcggta ccagaccaag ccggtcgcga
     2101 ccaatgatta gaaaaattgc aatctgcttg ctgaccattg ctttgctgcc aaccgtttac
     2161 tttgccgagg cgcagcagtc gaagcagatt tatcgaatcg cgattctgtc accagctaca
     2221 cggccacggc cggtcgtcga cgcacttcga caacgcttac gagagctcgg ttatacagaa
     2281 aaccagaacg ttgtgttcga agatcgttac gccgagttta aagtcgagcg tctgcccgaa
     2341 ttggctgccg agttggtcaa gctcaagccc gatgtcatct ttacgcattc gatcaccggc
     2401 gcaagggcgg ccaagcaagc cacttcaatt attccaatcg ttattggagc agccggtgat
     2461 ctggtaaaag acggcattgt ctccagcctg gcgcgtccag gtggaaatgt cacaggggta
     2521 actttgctaa gcagcgaact ggagggtaaa cggctcgagc tattgaagga aagcaatcgc
     2581 aagctctctc atgtcgcggt cttggtgaat tcggcaaact cggcctggga gaattatccg
     2641 caggttctcg aacccgccgg tcaggccctg aaggtgcgtc tcgcgcgcgc tgaggtgagg
     2701 caatccgccg accttgaagg cgcattcgcg gcaatgcgtc gcaatggagt cgatggcgtc
     2761 ttggtagtca gcgatccgat ctttcaaact tatcagcgtc gcatcgcgga gtttgcggcc
     2821 aggcagcagt tacccagcat ttcagaggtg ccgggcttcg ccgaggcggg cggtctgatc
     2881 caatatggtc tcagcattcc tgaaatgggg cgacaggcag cagtctacgt cgataaaatc
     2941 ctcaatgggg ccaacccggc cgacctgccg atcgagcggc cgacaaagtt tgaatttctc
     3001 atcaacttga aatcagctaa gcaattgggt ttgatcctgc cgccggcgat gctggcgcgg
     3061 gccgacaagg tcataaagtg ataatatcgg catctacaag gtaatcagac aaaaatgaaa
     3121 gtgcgtgact tgcccatctt gaagagcttt ggggtgcgtc gccggcgcga acggctgctg
     3181 cgcgattatt ttttgatctc cgtgctgctg ataggcggcg gcttgatcac cagcggcttg
     3241 gtcgagatct attatcgtta tcaagagagc caggagcagc tcgcgctgct gcagaaggag
     3301 atcgctgctg gggcagcttt caagatcgaa cagttcgttc aggagattca taatatcctg
     3361 aaaggagcga ccaagagccg cgacatcgct ccgaaaggtt taacgccgga gtttcgcttt
     3421 gaattggaga aactcttgat cattgcgccg gcggtcaccg aggcgcttgc gctcgacgaa
     3481 aacggcacca tccaagtaca ggcatcgcga ttgcgcacgg tgttgccgga cgctaagaaa
     3541 gacctctccg cctcgccgtc cttccgtcag gctagacaag gcaaatccta ctttggccaa
     3601 gtttattttg ttcgcggttc agaaccttat atgactatcg cggtgccgat cgagcggttt
     3661 gccggcgacg ttatcggtgt gcttcaggct gaagttaatt tgaaatacat cggcgacgtc
     3721 atctccaata tccgcgtggg caaagccggc cacgcttaca cgatcagccg ttccggtgag
     3781 ttaatagccc atcccgatat cagcctcgtg ctgcagcgcc gcaatgtgct ccatctggaa
     3841 caggccaggg cagcgtttcg accgaccacc cctggcggtc cggcttcgaa tgcaatcgtc
     3901 gccaccgatc tacaaggcaa agatgtgctc agctcgttcg ctttgatccc gattctggac
     3961 tgggccgtgg tgctcgagcg gccggtcgaa gaggcatacg aggcacttta tgcttcgatg
     4021 attcgaacct cgacactttt catgattggt ctcggcatgg ctctcatcgc cagcctgttc
     4081 ttagcccgcc gtgttgttcg cccggtgaga atcctgcgcc aaggtgtgga acgaatcggc
     4141 agcggcgatc tggcttatcg gctcgatctt aaaaccggcg atgagatgga aatacttgcc
     4201 gacgagttca acaagatgac atctcagctc caggagtctt atgccaattt ggagcaaaaa
     4261 gtcgaagcgc gcacgaagga gctcaccgaa tctttggagc aacaaaccgc cacgagccag
     4321 gttctaggcg ttatcagcag ttcgccgaga gacctgcaac cggtatttga aaccatgctc
     4381 gccaacgcca cgcgtttggg cagttccgac tacggcgcaa tttttcgatt cgatgggaag
     4441 atctttagtc tggtgacggc tcaatatgtt tcgccgcata tgaccgtatt ggctgatcga
     4501 acatttccgc cagacccgac aaccgcagtc ggccggcttg ggctggaaaa gaagatcgtg
     4561 cacattcctg atgtttcaat ggatcctaca tggaaccctc cggaagaata ccgtaaagag
     4621 ggtatacgga gcgttctcgc agttcccatg ctcaaggaaa ataatctggt cggagcgatc
     4681 gttgtttacc gcagagaaat tcaaccattc acggagagtc aaatcgccct gctcaaaact
     4741 ttcgccgatc aggccgtcat tgccatcgag aacgttcgtc tattcaaaga gctcgaagag
     4801 aagaatgaac agctcgaagc cgccaaccgg cataagtcgc agttcttggc gaacgtcagc
     4861 catgagctgc gcacaccgct gaactcgatc attgggttta cccggctggt gctgcgcaag
     4921 atcgagggac agattgagaa gctgcagaaa gaaaatctgc agaaagtgct gatcagcgcg
     4981 gaacacttgc tcaagttgat caacggtctt ttggatctgg ccaaaatcga agccggccgc
     5041 atggaagtct atgccgaaac attcaagatc gatgatgtag tccgggttgc aaccgcgacg
     5101 gtcgagccga tgctcagaaa tggcaatgtg cgcctggtta ctgagatgcc tcccgagctt
     5161 ccggcgatca aaacagaccg ggataaactc aagcaggtca ttctcaatct cttgagcaac
     5221 tcggccaagt tcactgaggc aggagaaatc aaagtcgctg cctggtcgga aaacggctcg
     5281 ttgaagctgg ctatttccga taccgggatt ggcatgaaca aagatgcttt gcactatatt
     5341 ttcgatgagt tccgtcaggt ggacatgtcg acgactagaa aatatggcgg caccgggctc
     5401 gggctaacta ttgtcaagcg actcattgat ttgcttggcg gcgagatcgg cgtcgagagt
     5461 gaagtaggca aaggttcaaa gtttaccatc accctgccgc tcaaccataa caactaaaca
     5521 gcttcgaatt ttcgatttgg gagccgcagg gaacaacaac cgtcgcgagc tggtatagaa
     5581 gaattcaccg cagtctaaat tcagctctgc cagcgaggaa tccatgggaa caaagaaaaa
     5641 aatactggtt gtcgatgaca ccgagttcaa tcgcgatctc gtggtccagc tgcttgaaga
     5701 cgaatttgaa ttagttatcg ccgaagatgg cgcggaaggc ctcaaacaaa ctcaatccga
     5761 aaggccggac ctcatcttga tggatctcgg catgccgatc atggatggct gggaggccac
     5821 ccgcaaaata aaggctaacg atgagctaaa gcatattccg attatcgccg taacatctca
     5881 cgcgatggcg ggcgacgaag tcgaggcgcg cagagccggc tgcgatgatt acctgcccaa
     5941 gcccatcgat gaagacgaat tattgaagaa gatcaggaaa tttctgttct gagttgagcc
     6001 gagcagaact caactcccga tcgaaccgaa aactgtagac ccgatacgct catgcccaac
     6061 aaaattctga tcgtcgacga cgaaccgttc aatctcgatc tgctcgagca agagttacag
     6121 gagcaaggct acgagatcga aagggccagt gacggcgctg aggcgctcga aaaactgccg
     6181 gtttttgtac ccgatgtcgt gctcctcgat tatatgatgc cgaggatgaa tgggctggag
     6241 gttctgaagc gccttcgagc cgacgagcaa tggcgaagtc ttccggtaat acttctcaca
     6301 gccaaggcgg cgcaggaaga taagatcacc gggctagatg ccggcgctga cgattacgtc
     6361 atcaaaccgt ttgatgcatt cgaactgttg gccagggtcc ggtcaatgct gcgcatcaaa
     6421 gagctgcacg attctttgga agaattaaac aagacattgt ctcagaaagt cgaacaacag
     6481 gtcggcgaga ttgaacggat gaagcggctc aaacgatatc tttcgccgca gatcgccgat
     6541 actttgatcg cgagcaatga tgagagcttg ttcaaaaccc accggcgcga gatcaccgtg
     6601 gtgttcctcg acctgcgtgg atttaccgcg ttctccgata gcgcggaacc cgaagaggtc
     6661 atggaattgc tgcgaactta tcatgccgcc atgggcaagc tggtgtttaa atacgaaggc
     6721 accctggagc gcttcgccgg tgacggcatc atgatcttct tcaatgatcc gataccctgc
     6781 gaagatcaca ccgagcgcgc gatccggatg gccacagaga tgcgtagccg agccaaagag
     6841 cttcgcagtg gctgggtgaa gaagggttat gatctagatt tgggcattgg tctggcctcc
     6901 agctatgcaa ccttgggaaa catcgggttc gagggccgca tggattacgg cgccgtgggt
     6961 aatgtcacca atctagccgc acgcctctgc gatgaggcta aaggcggaca gatcctgacc
     7021 aatcagagaa cgttaagcaa agtcgaagat ctaatcgagt tcgaagcttg cgaggagttg
     7081 catctcaagg gttttagccg gcccgtcgct gctttcaata tcatcaagct caaataatga
     7141 acgatccaaa accaaaccag cttacctggg atacttgctt caagactctc atttcgttcg
     7201 attgccctat caagaacttg acgttcttcc cacgccgacc ctacaatcca gccatccaag
     7261 atccgcaatg gcacatagaa ttttactcgt cgatgacgag ccttttaact tagacctgct
     7321 ggagcaagag ttagcggatc agggccattc gattgcaacc tgcgccagtg gggaggaggc
     7381 tctggagaag ttagcgggat ttcagcccga tttgattctg ctcgactaca tgatgcccgg
     7441 catgaacggt cttgaagtgc taaaagaaat ccgccgaaaa ggcctggaca ctccggtggt
     7501 catcgtcacc gcccatggta cgatcgagcg cgctgtagag acgatgaagg agggcgccga
     7561 agattttctg accaagccat tcgatcctga ccatctcgcc atcgtagtca agaaagcgct
     7621 cgagcggacc cggcttcgtg aagaagtaga gattctttcc gaacagctcg gcgctcgctt
     7681 taagatggtt cagggcagaa gcctgtcaat ggcgcgggcc gtgattgagg cccaaaaagc
     7741 tgcggtcagc aaatccacag tcctgctgct tggcgagagc ggcacgggca aagaaatttt
     7801 tgccagagcg attcataatt ggagtcaaag aaaatcagcg ccgttcatac cgatcaattg
     7861 cgtcggcctt tccaaagatc ttttggaaag cgagctgttc ggtcatgaga agggggcatt
     7921 tacaggcgcg caccagttaa aaaaaggaag agtcgagcta gccgatggtg gaacgatctt
     7981 tcttgacgag gtcggcgata tctccccgga gctgcaagcg aagcttctaa gatttttgca
     8041 agagcgcgag ttcgagcggg tcggcggaac acaccagatc agcgtcgatg tgagaatcgt
     8101 tgcggcaacc aaccgcaaca tcgacaaagc cgtgagcgag ggcacgtttc gtgaagatct
     8161 ttatcatcgg ttgaacgtca ttccgatcac cctcacgccc cttcgggaac gccgtgaaga
     8221 cattaccgag ctggccgccc attttttaca gcgattttct gttgaaacca agaagcggtt
     8281 ttctggaatc agcgaggatg cggaagcaac cctaatgacc taccattggc ccgggaatgt
     8341 gcgcgagcta gcgaatgtaa tcgaacgcgc agtcgttctc ggggacggcc ccgtcctgac
     8401 cattgatctc ttgccggcgc gcatcgccgg tctaaagcca accaatataa ctgccgtaac
     8461 gaaatcctat cacgagacaa tcaatcatca ccggcgcgac ctgatcttga aggcgctttc
     8521 tgaggctggc ggcaatcggg cagccgctgc caaggctctc ggtttgcaca gaactcattt
     8581 gctgaagctc attaaagctt tgcagctggg ctagagcttt catgtgaaca gttcaggtct
     8641 tacttaactg acgtcctcaa gaactcagtg acagagtttt gctgtattgg gcagtgcgca
     8701 tgcgcggata taagccccct gcgagaaggc acatggcccg agcaaaaata ctccgctcag
     8761 gctacccagt gcgaaagtgt gccaactctg ctgcctggta aagcggcaac gcatgggcgc
     8821 ggcgatgaat aaaacattct attcgtttct caaaagaaat aatctagcct gatcaactga
     8881 tattcagttt tgattttgtt ctcgacgaaa taaaaaaatt caaaaaatgt tttctaaaat
     8941 ggtatttttt tcttgacata caacttatag cgcccttata atccgatcag ctacaaaata
     9001 tgggagggtt tttgttctac ttctcttcgc ctgagtcacg ctttaactcc cctcgactag
     9061 agcccacccg ccgaatccta aaaggaggtg cctatggcaa ctaagaaaaa agcggcaaag
     9121 aaaaaagcca agaagaaaaa gaagtaacgt tttttgatgc ggggttgtgg tggtgaataa
     9181 accctcctag actctcaggc ggctagatag tcgttctggc tatctcgaaa acaggccctc
     9241 cggtgattgc cggggggctt tgtttttgtt gtatctcaac ggaatctctt cactcagaac
     9301 cgagcctagc tgcatcatcg atggctgatc gcgatcatac agctagatct aagatggacg
     9361 ccggatggcg catgcggaga accaggttac tcaaaccttc aacacaattc cggacattgc
     9421 gtcgattcag ccagttcgct acagtcatag ctatgaatta tgcgtttccc aggcgatcgg
     9481 ttttgttgct gctaatcata ccgacactcc tttcaacggc ttcgcgctac gcttggggac
     9541 agagcaagat caattacaag gtcattccaa atttagaggc gatgaaagat caagcgccga
     9601 cgccggattt cactttgccg aatttcgata gcaagaaaat ctcgttgaaa gatttccgcg
     9661 gcaaattggt ttttttgaat ttctgggcga c
//
LOCUS       DASUTG010000148         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_319678, whole genome shotgun sequence.
ACCESSION   DASUTG010000148 DASUTG010000000
VERSION     DASUTG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319678"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VF089_03380"
     CDS             complement(<1..1142)
                     /locus_tag="VF089_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6173032.1"
                     /translation="MGELQQTEAQERERNRQNELISTMLEPGFYPNCPAEVTHKETHI
                     SHLFFADDLVYKIKKAVRFSFLDYSTLAKRRYYINEELSLNRRLAPTVYLEVVPIGRD
                     VSGWRLGASENPLEFALLMRRLPDSRMLPVLLESAQVTPEMMRSLAQVLAGFHANAQQ
                     LDRAVAATHFDTVARQWRDNLKDVEQFIGDAIDLESFAAIKAFGDNFLDQNRGFISRR
                     AFDGWVRDGHGDLHCEHVCFAPEGIQIFDCIEFDPQLRLCDLTSEIGFLLMDLEARGG
                     ARWGEALLPRYRDLIDDSSLTVLLPFYKCYRALIRGKVEALRGGARAAAVRYFTIAGR
                     QTWSPYKPFVAVVCGLTGSGKSTLARELSERIGIDVLNSDVIRKELT"
     gene            complement(1399..1656)
                     /locus_tag="VF089_03385"
     CDS             complement(1399..1656)
                     /locus_tag="VF089_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173033.1"
                     /translation="MAIAIELILGAFTGLAAFFGGLMNWSFIMAGSASTNGMLFAIAT
                     WIVLAWRNAGWIGLDRWLLPMLGTPWKPGRLFHRNPAENVT"
     gene            2207..>2876
                     /locus_tag="VF089_03390"
     CDS             2207..>2876
                     /locus_tag="VF089_03390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6173034.1"
                     /translation="MQKQSDPSTTPNASVGVLVGGGPAPGINGVIGAVTLEARNRARK
                     VIGLYDGFQWLMRGDTTRTRELLKDEVSRIHFQGGSILNTSRANPTKKAEDVERVIAT
                     LDRLGIGYLVTIGGDDTMYAASQVAKYAAGRIRVCHVPKTIDNDLPLPGEIPTFGFET
                     ARQLGSALVQNLMEDSRTTGRWYFVVVMGRTAGHLALGIGKSTGATLTIIPEEFPDIV
                     HLESV"
BASE COUNT          702 a          825 c          742 g          607 t
ORIGIN      
        1 gtaagctctt tacgtatcac gtccgaattg agcacatcga tcccgatgcg ctcgcttagc
       61 tcacgggcca gagtcgactt tccgctgccg gttaaaccac aaacgacggc gacaaatggc
      121 ttgtatggac tccaggtctg ccggccggcg attgtgaaat atcttacagc ggcggcgcga
      181 gcgccgccgc gtaacgcttc gaccttgccg cgaatcaatg cgcggtagca cttgtagaat
      241 ggcagcaaca cagtgagact ggaatcatct atgagatcgc gatatcgagg cagcaacgct
      301 tcgccccacc gggcgccgcc gcgagcctcc aggtccatca gcaaaaagcc gatctccgag
      361 gtcaggtcgc acaaacgcag ttgcggatcg aattcgatgc agtcgaagat ctgaattcct
      421 tcgggcgcaa aacagacatg ctcgcaatgc aaatcgccat ggccgtcgcg cacccaccca
      481 tcgaatgccc ggcgcgagat aaagccccgg ttttggtcga ggaaattgtc tccgaaagct
      541 ttaatcgcgg caaaactctc gaggtcgata gcatcaccga taaactgttc gacgtctttg
      601 agattatcgc gccattgcct tgccacagta tcgaagtggg tggccgcgac ggcgcgatcc
      661 aactgttgag cattagcgtg aaacccagcc agaacttggg cgagggagcg catcatctcc
      721 ggtgtgacct gcgccgactc cagcagcacg ggcaacatgc gggaatccgg caagcgccgc
      781 atcaacaggg caaactccag aggattctca ctagcaccaa gccgccatcc tgatacgtcg
      841 cgcccgatcg gcaccacttc caaataaacg gttggcgcca accgtcggtt aaggctcagc
      901 tcctcattga tgtagtagcg tcgcttagcg agagtggaat agtccagaaa agaaaatcgg
      961 accgcctttt tgatcttgta aaccaaatca tcggcgaaaa agagatgcga gatgtgggtt
     1021 tctttgtggg tcacttcggc cgggcagttg ggataaaagc ccggttctaa catcgttgag
     1081 attaactcgt tttgccgatt ccgctcccgc tcttgggctt ccgtctgctg cagttcaccc
     1141 atgcgcttcc tctaaatcta aactgcgcaa aaacatgcca cgaagagaag ccgatatcga
     1201 ggagctttgg tgtttaccca cgtggtgtgc ccgtttactt cgcacacgag cgatgtgttg
     1261 cttctcaact gccgcagtgc gtctttgaga tgaacgtgag cggcctaacc ggagccggcc
     1321 tggatcactg agacctaaag ttcctttaac tcgcaccggc ctgatcttca gcactcacag
     1381 ttcaaaactc ccggtgaatt acgtcacatt ctcagccggg tttcggtgaa acaatcgacc
     1441 gggcttccag ggagtgccta gcatcggcaa cagccagcga tcgaggccga tccagccggc
     1501 atttctccag gctaagacaa tccacgtggc tatagcgaac aacattccat tggtggaagc
     1561 cgagccggcc atgatgaagc tccagttcat taatccaccg aagaaagctg ccagcccggt
     1621 aaacgcgccg agaatcagct ctatggcaat cgccaacccg ccgaagatga tgatctttga
     1681 aaaccaggtg tgggcctcgg aagttcacca gtaactcgat aaaggaccga taccaatcgt
     1741 acgagatcgc cggcttgggc gccattttga ggcctctctg ccaatagctt aacagcgcct
     1801 ctccattatc ctccacttcg ggtcgatgaa cttgtgccat ccagcctccc accacgccca
     1861 gccaagataa agacgcaatg gcaactaaag ccatgccagg cgcgcgtctg caaagagcag
     1921 tcgcgcaatc ggcggttcct caatgatgat ggtttctttg ttcatcctca cacctcccta
     1981 actttagccc acatagcacc ccaagaaacc ctccgcaacg gttgtaccat taaatcctcg
     2041 cactgaggcg ggggattcaa attaaatatt tggcaaattt gcggatcgtc cgtctaagtt
     2101 ttaaaaccta agaaactttg ccgcagacat cttggatttg cgtgccaaaa cgataaactc
     2161 cgtgtagcat tttactaagt agatttgcag tgcggcaggt ttaatcatgc aaaaacaatc
     2221 agacccttcg acaactccca acgccagcgt tggcgtgctc gtaggtggcg gaccagcgcc
     2281 cggcatcaat ggcgtcatcg gagcagttac cttggaagcg cgcaatcgcg cccgcaaggt
     2341 gataggtctc tacgatggat ttcagtggct gatgcgtggc gacacaacgc gcaccaggga
     2401 actacttaaa gatgaggtct cgcgcatcca ctttcaggga ggctcgatcc tcaatacgtc
     2461 acgcgcgaac ccaaccaaaa aggcggaaga tgttgaacgg gtgatcgcca ctctggaccg
     2521 gctaggcatc ggttacttag tgaccattgg cggtgacgat accatgtatg cggcaagcca
     2581 ggttgcgaaa tacgccgccg gacgtatccg ggtctgccat gttccaaaga ctatcgacaa
     2641 tgatttaccc cttccgggcg agatacccac attcggcttc gaaacggccc gtcaactcgg
     2701 cagtgctctg gtgcaaaact tgatggaaga ctcccgcact accggacgct ggtatttcgt
     2761 cgtggttatg ggacgtacag ccgggcatct ggctctcgga atcggcaaat caacaggcgc
     2821 taccctgacg attatcccgg aggagtttcc cgacatcgtg catctggaaa gcgtct
//
LOCUS       DASUTG010000149         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1357932, whole genome shotgun sequence.
ACCESSION   DASUTG010000149 DASUTG010000000
VERSION     DASUTG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357932"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VF089_03395"
     CDS             complement(<1..898)
                     /locus_tag="VF089_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6173035.1"
                     /translation="MAGNNYDVIVIGAGNAGLTAALAAREQQMRVLLLDKCPKPSRGG
                     NTRFSGGGFRFTYNNLDDMRPMLPELTDEEAARMEVGTYSNAEFFEDVMQVTEYAADK
                     KLTNILVDRSYDTARWLTDMNVKWILSTSTHAVKMGGKIKFPSGRVISVNDGGLGLVE
                     MLFPTAEKNGIEIIYEAKATALTVDAKGKVSAVRVQTAEGLVDFPSRAVVLAAGGFEA
                     NPEMRARYLGAGWDLVKVRGSRYNTGEVLGLALSFGAQAVGHWSGCHAVLVDAEAPDV
                     ECAYEHRYSYPYGIMVDINGRRF"
     gene            complement(1085..2437)
                     /locus_tag="VF089_03400"
     CDS             complement(1085..2437)
                     /locus_tag="VF089_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6173036.1"
                     /translation="MDAVEEKLVRFVDSFAFADIEAPVLHAIKLRFIDTMGCALAGYD
                     EAPAKIARRLAAQVRSSEEAGMIGTTAKTSAEMAAFANTTMIRCLDLNDDYFGKDGPH
                     PSDLIGAVLPGSEIAHGNGSSFVAAVAVAYEVLCTLVDSVGIRERGWDYVTYTALAAA
                     LGSGKAMGLSQESLRDSLSLAATANCSLGQTRLGELSMWKGMASANACRNGLFAALLA
                     RAGVSGPFLPFEGKGGFLRQVCGSLDLSRLGATPLRAGIVYLKNWPVFYSAQGAVDAA
                     IALREKVRPDEIKTLVVESYQRLIGRGATDPEKWTPQSRETADHSVPFCVAAALLDGG
                     VTAQTFDAARFLDRDAIELMAKITLREDPEFTKQYPEKWNCRVVAETFSGARHEVHVA
                     YPKGHPENPFSDADVEDKFLRLAEPSLGRSRCQKFIDWAWRLEQAEEIRELFPLLAIS
                     "
     gene            complement(2454..3464)
                     /locus_tag="VF089_03405"
     CDS             complement(2454..3464)
                     /locus_tag="VF089_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173037.1"
                     /translation="MSGKIRMLIILMAVLVVGIFCSPASAQTIEELYRKAAQEKTINF
                     YGTLAQVNAEKILPVFEKRYPGVKLNHVDITSDSLVARATAEARGGKTIGDIFQAPLE
                     TAIQMNDQKLLLEVTLPEYSAYPAIMKGPYWVASNLQFIVVAWNTNLLKKADEPKWID
                     DLADAKYKESLVAEPRDFEFLMGIAKHKFKNDEKAIALLRAIAANGVEFHKGHSQLTE
                     LLTGGQAKLCVTCFAHQFPGRMKKGAPVNFLLSEGIGSMNATAIFKDAPHPNAALLFA
                     RWAASEEGQKVYAEGGRAPAHPKVKPIDPIRPERLYPLGVDDIKEYPKYEKIWKDVFK
                     LR"
     gene            complement(3515..>4014)
                     /locus_tag="VF089_03410"
     CDS             complement(3515..>4014)
                     /locus_tag="VF089_03410"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173038.1"
                     /translation="NIDPRQVTKITVELNEGILLHGGTIYQPKEVIEAQFSLRFSLAL
                     RFLTGNNDLQHYLDPAMWSDPAILEIGRKIDLLADPTATGPRRFACRMTILLLDGCEM
                     SGSLAAPKGSYKNPLAPGELQDKFYRLGRTVLDDERLGSIVAAVEWIESAPNVAALST
                     LMTAA"
BASE COUNT          781 a         1228 c         1138 g          867 t
ORIGIN      
        1 cgaagcgcct gccgttgatg tcgaccatga ttccgtacgg gtaggagtag cggtgctcgt
       61 aggcgcattc gacgtcgggc gcctcggcgt cgaccagaac ggcgtgacag ccgctccaat
      121 ggccgactgc ctgtgcgccg aaactcagcg ccaaaccgag aacctcgccg gtgttgtagc
      181 gcgagccgcg gactttcacc agatcccagc ccgcgccgag atagcgcgcg cgcatctcgg
      241 gattggcctc gaagccgccg gcggccaaga cgacggcgcg gcttgggaaa tcgaccagtc
      301 cttccgccgt ttgcacccgc acggcggaga ccttgccttt ggcatcgacg gtcaaagcgg
      361 tcgccttcgc ctcgtaaata atttcgatgc cgtttttctc cgccgtcgga aacagcatct
      421 cgaccagtcc aaggccgccg tcgttcaccg atattacccg gcccgaggga aacttgattt
      481 tgccgcccat cttcactgcg tgggtgctgg tggaaagtat ccatttaacg ttcatgtcgg
      541 tcagccaacg cgccgtgtcg taagatcgat cgacgagaat gttggtgagc tttttatcgg
      601 cggcatactc ggtcacctgc atgacgtctt cgaagaattc cgcgttggag taggtgccga
      661 cttccatcct cgccgcctct tcgtcggtca actccggcag catcggacgc atatcgtcca
      721 gattgttgta agtaaaacga aacccaccgc cggaaaatcg tgtatttccg ccgcgcgatg
      781 gtttgggaca tttgtccaag agcagcacgc gcatttgttg ttctcgtgcc gccagagccg
      841 cggtcaatcc cgcgttgcct gcgccgatca cgatcacgtc ataattatta cctgccataa
      901 gtctcgaaac ctcctcatta ctccgtcctt tcaagttctt ctttgggctg ccagtgcttc
      961 tccgtcattc ccgcatgctt taagcgggaa tccaggcgaa acgagaactg gacccccgat
     1021 aaaaacgttc gggggcgacg actgcagaat gcaatctcat tgcttgcatt cggaaacctc
     1081 cgcttcatga aattgccagc agcggaaaga gctcacggat ctcttccgcc tgctccagcc
     1141 gccaagccca atcgatgaac ttttgacagc gacttcttcc gagagacggt tcggccagcc
     1201 gcagaaattt gtcctcgacg tcggcgtccg agaatggatt ctccggatgt cctttcggat
     1261 aggcgacatg gacttcgtgc cgcgcgccgg aaaatgtttc ggcgacgacg cggcagttcc
     1321 acttttccgg atactgtttc gtgaactccg gatcttcccg cagagtgatt ttcgccatca
     1381 gctcgatcgc gtctcggtcg agaaaccgcg ccgcatcgaa ggtttgcgcc gtcacgccgc
     1441 cgtcgagcag cgctgccgcc acgcagaagg gcacgctgtg atcggcggtc tcgcgtgatt
     1501 gcggcgtcca tttttccggg tctgtggcac cgcgcccgat caaacgctga taggattcca
     1561 cgactagcgt tttgatttcg tcgggtctga ctttctcgcg cagcgcgatc gctgcatcga
     1621 cggctccctg cgcgctgtag aagaccggcc agtttttcag ataaacgatt cccgcgcgca
     1681 aaggcgtcgc gcccaggcgg gagagatcca aagagccgca gacctgccga agaaagccgc
     1741 ccttcccctc gaagggcaaa aagggcccgc ttaccccggc gcgtgcgagc agagccgcga
     1801 agaggccatt gcgacaggca ttggccgacg ccataccttt ccacatcgaa agctcgccca
     1861 gtcgggtttg tcccaacgaa caattcgccg tcgccgccag gctcaaagaa tcgcgcaacg
     1921 attcctgcga cagtcccatc gctttgccgg agccgagcgc cgcggccaat gcggtgtagg
     1981 tgacgtaatc ccagccgcgc tcacggatgc cgaccgagtc gacgagagtg caaagaacct
     2041 catacgcaac cgcgacggcg gcgacgaacg acgagccgtt tccatgcgcg atctccgatc
     2101 ccggcagcac tgcgccgatc agatcgctgg ggtgggggcc gtctttgccg aaatagtcgt
     2161 cgttcaagtc gaggcagcgg atcatggtcg tgttggcgaa cgccgccatc tccgccgaag
     2221 ttttggcggt ggtgccgatc atgccggctt cctcggagct gcgcacttgt gccgcgaggc
     2281 gccgcgcgat cttcgccggc gcttcatcgt agcccgccag agcgcagccc atcgtatcga
     2341 tgaagcgaag cttgatggcg tgcaggaccg gtgcttcgat atcggcaaaa gcaaacgagt
     2401 cgacaaagcg aaccaatttt tcttcgaccg cgtccatcgg taagctcgta tctctagcgc
     2461 agtttgaaaa catctttcca gattttttcg tacttgggat actctttgat atcgtcgaca
     2521 cccaacggat agagccgctc cggccggatc ggatcgatcg gcttgacctt gggatgcgcg
     2581 ggcgcccggc cgccttcggc gtaaaccttt tgcccttctt cgctggccgc ccagcgggcg
     2641 aaaagcagcg ccgcgttcgg atgaggcgcg tctttaaaga tcgccgtcgc gttcatcgat
     2701 ccgatgcctt cgctcaacaa gaaatttacc ggcgcgcctt tcttcatccg cccgggaaat
     2761 tgatgcgcga agcaggtcac gcagagcttg gcctgaccgc cggtcagaag ctcggtcaac
     2821 tgcgaatgac ctttatgaaa ctccacgccg ttggcagcga tggcgcgcag caaagcgatc
     2881 gccttctcgt cgtttttgaa cttgtgcttg gcgatgccca tgagaaactc gaaatcccgc
     2941 ggctcggcca ccagcgactc tttgtacttc gcatccgcca ggtcgtcgat ccatttcggc
     3001 tcgtccgctt tcttgagcag gttggtgttc caggcgacga cgataaactg caggttcgag
     3061 gccacccaat acggtccttt catgattgcc gggtaggcgg agtactccgg cagcgtcact
     3121 tcgagcagca gcttttgatc gttcatttgg atcgcggttt ccagcggcgc ctgaaaaata
     3181 tcgccgatgg ttttgccgcc gcgcgcttcc gccgtagcgc gggcgaccag gctgtccgaa
     3241 gtgatgtcca cgtgatttag cttaaccccg ggatagcgct tctcgaacac tggcaggatc
     3301 ttctcggcgt tgacctgcgc cagggttccg tagaagttga tggttttttc ctgggcggcc
     3361 ttgcggtaaa gctcctcgat cgtctgcgcg ctcgccggcg agcaaaagat gccgacgacg
     3421 agcacagcca tgagtatgat caacattcgg attttgccgc tcatcaactc atcctccatt
     3481 ctcaagtgat tccgagtgcg ggaaatcgtc ggcatcacgc cgccgtcatg agcgtgctca
     3541 gcgccgcgac gtttggcgcg ctttcaatcc attccaccgc agcgacgatc gaaccgagtc
     3601 tttcgtcatc cagaaccgtc cggcctagcc ggtaaaattt gtcctgcaat tcgccgggag
     3661 ccagagggtt cttataactg cccttgggcg ccgccagcga accgctcatt tcacatccgt
     3721 ccaaaagcaa aatggtcatc cggcaagcga aacggcgcgg accggttgcc gtagggtcgg
     3781 caagcaggtc gattttcctg ccgatctcca ggatcgccgg atcgctccac atggccgggt
     3841 cgagataatg ctgcagatcg ttattgcctg taaggaatct cagcgcgaga gaaaagcgta
     3901 ggctgaattg ggcttcgatg acttccttgg gctgatagat ggtgccgccg tgcagcagga
     3961 ttccttcatt gagctcgaca gtgatcttcg taacctggcg cgggtcgatg ttgt
//
LOCUS       DASUTG010000150         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_467998, whole genome shotgun sequence.
ACCESSION   DASUTG010000150 DASUTG010000000
VERSION     DASUTG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467998"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF089_03415"
     CDS             complement(<1..330)
                     /locus_tag="VF089_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6173039.1"
                     /translation="MAWVYRLGLWRRLVNRIIRALLRIRVSPPHTYLLTVGGRKSGQP
                     YSTPVTLVEEAGERWLVAPYGEVSWVRNARAAKQVTLSRGRRFDTVRIVELAAEDAAP
                     VLKKYLAQ"
     gene            414..1772
                     /locus_tag="VF089_03420"
     CDS             414..1772
                     /locus_tag="VF089_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173040.1"
                     /translation="MSKVYQSVRGVVQGALHRPKPQMVSARVKANDGNLEDAIQEFEQ
                     IFVDGIGRLKAAVSDDQAVAASEAQHAQDVAETLKANIAALEARLRDTEDTLDKKDVA
                     SQKLEENLRTEIGDLQSTLKKKQEDLETRDSEINDLKSKTDILVEQATHSELELQHAK
                     REAAIAAQHAEQLIEGLKANITVLEARVRQTEDAIHKQDVASQKLEEGFRTEIRDLQS
                     TLRKKEEDLETRESEVNDLKSKINVLQEQVTHWELVTEQAQGHAASESQHAEEVIQGL
                     RIKIARLETQLNQTEQIVGGTDSTLKGLDHDRNIQAIDLDAQLEPKKNGINASGAEAS
                     VDIKAQANGAMAGEQLQTAEENPTTLPIPATAVTPNATIAAPETVSQDAFNRLIAEFG
                     ELTNVMGSIASLIVRDHVRALGESMEEFPQARLTKLLESLSKEISDDELKADFCERFA
                     NL"
     gene            complement(2300..3553)
                     /locus_tag="VF089_03425"
     CDS             complement(2300..3553)
                     /locus_tag="VF089_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6173041.1"
                     /translation="MRRSLFLLTMLVFVGCAKVLAVRKIPEVTLGDAAFFRTIQAHTG
                     APIVGGNRVEVLLNGDETFPRMLRDIKSAKSTITFAQYLYEDGSIADELAQAFAERCR
                     SGVKVAVLLDRHGSGKVPADIIRTMKDGGCHVEYFRQIEADGIIFPWRLLRYNYRSHR
                     RVLVIDGRVGFTGGYGVSDAWTGNGRMPKHWRDTNVRIEGPVVSFLQTAFAESWLETT
                     GIAIGGNDYFPPLDHAGTTQAQIVKSSPLGGSFQNYMLFLLSINSAKKSIFITNPYFI
                     PDRVMTDALVKAAGRGVRVIILTPGTIDNRFTYTASRSHYGPLLLGGVEVFEYKAAFM
                     HAKTIVVDGLWATIGSTNIDNRSFALNQEINLTVHDSTVARHLEEIFHQDLKYSEPVT
                     YEQWQSRGVFERLFEFFSFPIKEQL"
     gene            complement(3952..4197)
                     /locus_tag="VF089_03430"
     CDS             complement(3952..4197)
                     /locus_tag="VF089_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173042.1"
                     /translation="MNAEITLKDDEKEFLLQRAPEGSPARAILTGARPDDHQPALVSF
                     SCNEQTAHALLEIAEHHCGSAWRVMHYQMSRLGLLHH"
BASE COUNT         1131 a         1130 c         1119 g          994 t
ORIGIN      
        1 ttgagctaga tattttttta gcaccggagc ggcgtcctcg gctgcgagtt ccactattct
       61 tacggtgtca aatcgccgac cgcgcgatag ggttacctgc ttggccgcac gagcgtttcg
      121 tacccagctc acttcgccat atggggccac tagccagcgt tctcctgctt cctccactag
      181 tgtcaccggg gttgagtacg gttgaccgct ctttctgcct cccacggtca gaagataagt
      241 gtgcggggga ctcacgcgga ttcgcagcaa ggcacgaatt atccgattga ccaatcggcg
      301 ccacaatcct aaacggtata cccaagccat ttgctgatct tgccttcggg ctacccaatt
      361 cctctattga caactctaga caaatctaga cactgaattg gcccggagga ttaatgagca
      421 aagtatacca atcagtcaga ggagtagtgc aaggcgcctt acatcggccg aagccccaga
      481 tggtttccgc tcgggtgaag gctaatgacg gcaatttaga ggatgcaata caggagtttg
      541 agcagatctt tgtggatgga atcggcaggt tgaaggcggc ggttagtgac gatcaagctg
      601 tggcagcgag tgaggcccag catgcccaag acgttgccga gactctcaag gcaaacattg
      661 ctgccctaga agctaggctt cgagatacgg aggacaccct cgacaagaaa gacgttgcta
      721 gccaaaagct ggaagaaaat cttaggaccg agatcggcga tttacaaagt acactcaaga
      781 agaaacaaga agacctggaa actcgagatt ccgaaataaa cgatctcaaa tccaaaacag
      841 atatcctggt agagcaggca acgcactcgg agcttgagct ccagcacgca aaacgagaag
      901 cggcgattgc ggcccaacat gccgagcagc ttatcgaagg cctcaaggca aatatcaccg
      961 tgcttgaggc aagggttaga cagaccgagg atgccatcca taaacaagac gttgccagcc
     1021 aaaaactgga agaaggtttt cgaaccgaga tccgtgattt acagagtaca ctcaggaaga
     1081 aagaagaaga tctggaaact cgggaatccg aagtaaacga tctcaaatcc aaaataaatg
     1141 tcctgcaaga gcaggtaacc cactgggaat tggtgaccga gcaggcacaa ggtcacgcgg
     1201 cgagtgagag ccagcatgcc gaggaggtga tccagggcct gaggataaag atcgctaggc
     1261 tggaaaccca gcttaatcag acagagcaga tcgttggcgg aacggattcg accctcaaag
     1321 ggctggacca cgaccgaaat atccaggcaa tcgatctaga cgcccaatta gaaccgaaaa
     1381 agaatgggat aaacgccagt ggcgcagaag cttcggttga tattaaggcg caagctaacg
     1441 gagctatggc aggcgagcaa ttgcaaacag ccgaagagaa tccgaccact cttccgattc
     1501 cggcgacggc agtcacccct aacgcaacga tagcagctcc ggaaaccgtg tcgcaggacg
     1561 cctttaaccg tttgatcgct gagttcggtg agctcaccaa tgtcatggga agcatagcat
     1621 ccctgatagt ccgcgatcac gtgagggcgt tgggcgagtc aatggaagaa tttccgcagg
     1681 cgcggcttac aaaactcctt gagtctctta gcaaagagat atctgacgac gagctgaaag
     1741 cggatttttg cgagcgattt gcgaacttgt agtccccgga gaacaagctc cgtcccatcg
     1801 acatacacgc gatattgttc aagtactaaa gctcaacgac ctttcagttc aatcccggat
     1861 caagaacagc tttgtccgct tgccccatgg cttccgctca tcctcgataa agctagtatc
     1921 tgcatgaaca caggttataa atcataacct gtcacgtaaa agtccgtgtt aaacgttgtc
     1981 tacaagagct agcccgttgg ttcctactgg tacttcggtg acaaagaaca ctagcgtggc
     2041 aaggacttgg cgttttctcc ggagggtttt gctagggctg cctggtgtgt cggctgcgag
     2101 ctgcaccaac aaaggatctt gggctcgttg tcgcagcgac tactcgtttg cctgacgcac
     2161 aggtcgtgtc gattattctt gcacggccga catatccaat acattgcttg tttagtccta
     2221 aaccggaacg ctaagtagtt gtcattggca cgagccgccg aagacgcagt cagatcaaac
     2281 tgtatgttgt cgcattaaac tatagctgct ccttgattgg aaaagaaaag aattcaaaca
     2341 gtcgttcaaa aacacctcgc gactgccact gttcgtaagt cactggctcg gaatacttta
     2401 aatcctggtg aaagatttct tccagatgac gcgccactgt gctgtcatga acggtaagat
     2461 tgatttcctg attcaaggcg aaagatcggt tgtcgatatt ggtactgccg atagtcgccc
     2521 ataggccgtc gacgacgata gtcttggcat gcatgaacgc ggctttgtac tcgaaaacct
     2581 ccacgccgcc caagagtaaa ggaccgtaat gactacggct cgccgtatac gtgaatcgat
     2641 tgtcgatggt accaggtgtc agaataatta cccgtactcc ccggccggca gctttcacga
     2701 gagcatcggt cataactcta tctggaatga agtatggatt ggtgatgaaa atggactttt
     2761 tagcggagtt gatcgagaga agaaagagca tgtaattctg gaagctgccg cccagcggcg
     2821 aactcttgac gatttgtgct tgcgtggttc cggcatgatc aagaggtgga aaatagtcgt
     2881 ttcctccaat agcgatccct gtcgtttcaa gccagctctc ggcaaaagcg gtttgcagaa
     2941 agctaactac cggaccttcg attcgcacat tggtatcgcg ccagtgcttc ggcatgcgcc
     3001 cattgccggt ccatgcgtca ctcactccat agccgccggt gaatccgact cggccgtcga
     3061 ttaccaaaac gcgacggtgg ctcctgtaat tgtagcgcaa caaacgccat ggaaagatga
     3121 ttccgtccgc ttcgatttga cgaaagtatt cgacatggca gccgccatcc ttcatcgtgc
     3181 gaatgatgtc ggccgggact ttacccgatc catgacggtc tagaagaacg gctactttga
     3241 cacctgatcg gcagcgttcg gcgaaagcct gcgccagctc atcggctata gaaccatctt
     3301 catagagata ctgcgcgaat gtaattgtag atttagcgct tttgatgtcg cgtagcattc
     3361 tcggaaaagt ttcatcgcca ttaagtagga cttcaacgcg gttcccgccc actatgggtg
     3421 ccccggtatg ggcttggatc gtgcggaaga aagctgcgtc accgagcgta acctcgggaa
     3481 ttttgcggac ggctaaaacc ttcgcgcagc caacgaacac cagcatcgta agcagaaaga
     3541 gcgatcttct cattgggctc attagcttga acaccatttc aggcttaacc ctcgagctgt
     3601 gccgacaaaa gctgatgaca tttccatcga tttctgcccg tactcggctc tcgctccggc
     3661 gcgatcatac gaatcccgaa cggttgataa tgaccgtggc atctcactgc tccggaagtc
     3721 atcgcgttac cctcgtcaat ccactgtcgg gcgacattta ggataccggt cactgccctg
     3781 gccttaattt ttgcctgcga aatgtgtcga agacagtcga gcttgttctg cgacttgatt
     3841 ccatcgcaca attgtggcat cggatgacca tcattgccga gcgcatccca ccgtgcgtga
     3901 acccataatt ttgtattctt tatgaattgc cgcgagcatg tgttgtttac gttaatggtg
     3961 gagaagaccg agcctgctca tctggtaatg catcactctc caggcggagc cacagtgatg
     4021 ttctgcaatt tcgagcagcg catgcgccgt ctgctcatta cagctaaaac ttactaacgc
     4081 cggttgatga tcgtcggggc gagcccccgt taaaatcgct cgcgccggtg agccttcggg
     4141 agctctttgc agtaagaatt ctttctcatc atccttcaag gtaatttctg cgttcataaa
     4201 gtttcctcag atacaacgcg gctgcccgca tacacagtgg cgatcaatcc agttcgacga
     4261 ccgggatact catattccac ttccctacac tcacttgaca gtgcattaag aatgcctgct
     4321 tccagctagt cattagcgac cggccaggat aggacacgag aggtcatgga ctga
//
LOCUS       DASUTG010000151         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1506431, whole genome shotgun sequence.
ACCESSION   DASUTG010000151 DASUTG010000000
VERSION     DASUTG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506431"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VF089_03435"
     CDS             <1..683
                     /locus_tag="VF089_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6173043.1"
                     /translation="NKERIPVPFEKVRDQVHNYLREQKITERKNEFFKMLRAKGKVTT
                     YLKAPELVRVDVSVNGSPFKGSERAQVTIVKFEDFQCPYCKAVQPNYQEVLKRYDGKV
                     RLVHKDLPLEQIHPQARQAAEAARCADDEGKFWEYHDKLYASSPKAAPEDLKLYAKDL
                     GLKQDLFDECLISGKYRATIQKDLNDAASLGLTGTPTFFINGRELSGAQSVEAISQII
                     DEELSRAN"
     gene            complement(898..1632)
                     /locus_tag="VF089_03440"
     CDS             complement(898..1632)
                     /locus_tag="VF089_03440"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173044.1"
                     /translation="MPETEKEYLVGTVRPTNRPEAGQEIDLDERIPAKIKFVTLTMNF
                     TQGTEEEFRTSMPGYEAKSAELANMGAHLVRPSGAPPFMLLGYKGEQEIISSWEKKYG
                     VQMFTSGQNHVRALRTMGIKKFVGASYFPEKMNAIFARYFTEAGFQVLAMEGIDSPFA
                     EVPNVPPENIFQFMKGIAAKHKDAGGIYMLGSAWRTLDIIDPLEKDLSLPVVHAGPAR
                     CWETQLRLGLRYPIIGYGRLLAEMPG"
     gene            complement(2135..>2751)
                     /locus_tag="VF089_03445"
     CDS             complement(2135..>2751)
                     /locus_tag="VF089_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEX6173045.1"
                     /translation="NGNHRLSALRTLGARSVTAIVVPEPSTAYKILALNTEKSHNLRE
                     RALEVIKMYQELAMLDKADEDSYALEFEDAALITLGLCYLEQGRFSGGAYHPILHRVD
                     AFLKKPLSAALEVRRERSKMLMDLDNLVCERVDTLKKRGLTSPYLKSFVVARINPIRF
                     RPKDAAPLSYNDVLERMTKAIDKFDPEKIRMEDLAKSGGAPAET"
BASE COUNT          620 a          719 c          714 g          698 t
ORIGIN      
        1 aaaacaaaga gcgaatcccg gtcccattcg agaaggttcg cgatcaggta cacaactatt
       61 tgcgcgagca gaaaatcacc gaacggaaga atgagttttt caaaatgctg cgtgccaagg
      121 ggaaggtgac tacttacttg aaggctccag agctcgtgcg ggtggacgta tcagtcaatg
      181 gatctccgtt caaaggatcc gaaagggcgc aagtcacgat tgtaaagttt gaggattttc
      241 agtgtccgta ttgtaaagcg gttcagccga attatcagga ggtgcttaaa cgctacgacg
      301 gtaaggtccg attggtccac aaggatctgc ctttggagca gattcacccg caggcgcggc
      361 aggcagcgga ggcggctcgc tgcgcggacg atgagggcaa gttctgggag tatcatgaca
      421 agctttatgc atcgtcacct aaagcggctc cagaagattt gaagttatac gccaaagatc
      481 tcggcttaaa gcaggatttg ttcgatgaat gcctgatcag cggcaagtat agagcgacaa
      541 ttcaaaagga tctgaacgac gcggcgagtt tagggttgac cggaactcct acattcttca
      601 taaacggtcg tgagctgtcg ggcgcgcagt ctgttgaagc aatctctcag ataatagacg
      661 aggagctgag tcgcgctaat tgaagcaggg gaacgtcgca gattgtgatg ttcgccttga
      721 ttctaggcca gtggagtcgt gcgaagtcac cccattatgg gatcccggct cgaccctctg
      781 tgtcatctag gatggggtgc gggagttgct ccagtgtttc gcacttctaa actccgaaaa
      841 acgaatgagg gtacctttgt tccttgaccg cgttcgattc aggcccacat acaccaatca
      901 ccccggcatc tccgccagca atcttccgta acctataatt gggtagcgca ggcccaatct
      961 tagctgcgtc tcccagcatc gtgccggtcc cgcatggacg acaggcaggc tcaggtcctt
     1021 ttcgagcgga tcgataatgt cgagggtcct ccaggcggaa ccgagcatgt agatgccgcc
     1081 tgcgtctttg tgttttgcgg cgatgccttt catgaattgg aaaatgtttt cgggtggaac
     1141 gtttgggact tcagcaaacg gagaatcgat tccttccata gctaacacct gaaaacctgc
     1201 ttcagtaaag tagcgcgcga aaatagcgtt catcttctcg ggaaaatagc tggcgcccac
     1261 gaatttcttt attcccatgg tccgtagcgc gcgcacatgg ttctgcccgg aggtaaacat
     1321 ctgaacgccg tatttcttct cccaactgct gattatttcc tgctcccctt tgtaaccgag
     1381 cagcataaac ggcggtgcgc cgctcgggcg caccaggtgc gcgcccatgt ttgctagctc
     1441 ggcagacttc gcctcatagc ccggcatcga cgttctgaac tcttcctctg taccctgcgt
     1501 gaaattcatc gtcagagtta caaacttgat tttcgcggga atcctttcgt cgagatcgat
     1561 ctcctgaccg gcctcagggc ggttagtggg tctgacggtt ccgacaagat attctttctc
     1621 agtctcaggc atgggtttat ccttccttgt attatgcccc taacggctgc tgttttcgtg
     1681 gagacgtcgg cagttcttcc tctgtcatgt cacctctagg gtattcggac cttccgcttc
     1741 cctgttgggt gactgccttt actttcatga ctactgggat gagaatgctc cccggttctg
     1801 atcgagtcgc ctggcggcaa tgactaagtt cggctgaacg tatctcgaaa atgttccgac
     1861 ttccggggaa agacctcggt gatgctcgtg gtttatacta ttgccgactg ccattctgat
     1921 tctgaccggt tccagcaaaa taggttgcta attccagttg cgaacctttt ccggagtttc
     1981 tcctgcgcaa gccatattag tgcttcctca ggttacgccg tacaccgaac cgctgagcga
     2041 caccaaaaga gccacgctgt ttgcgcgatg cattttggag acgcgagaaa ttgagccggc
     2101 cccgtagcaa cagttttata ccgaataaca attctcatgt ttccgccggt gcgccgcctg
     2161 acttggccaa atcttccatc ctgatctttt ccggatcaaa tttatcgatc gctttagtca
     2221 tccgttccaa cacgtcatta tagctgagcg gcgcagcgtc ttttggccgg aagcggattg
     2281 ggttgatgcg ggcgacaaca aagcttttaa gatagggact ggttagacct ctcttcttga
     2341 gagtatcgac tctttcacaa accagattgt ccaaatccat caacatcttc gagcgttctc
     2401 ggcgaacttc caatgccgcc gacaacggct tctttaggaa agcatcaacg cgatgaagaa
     2461 tcgggtggta tgcgccgccg ctaaagcggc cttgttccag atagcacaaa cccaaggtga
     2521 tgagcgccgc gtcttcaaac tcaagcgcat agctatcctc gtccgcttta tcgagcatag
     2581 ccagttcttg atacatcttg atcacttcca gggctcgctc tcggaggtta tggctctttt
     2641 ccgtgttcaa ggcgagaatc ttataagcgg tcgatggttc cggcactact atcgcagtga
     2701 ccgacctggc gcccaaagtg cgcagcgccg atagacgatg attgccgttc g
//
LOCUS       DASUTG010000152         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_468744, whole genome shotgun sequence.
ACCESSION   DASUTG010000152 DASUTG010000000
VERSION     DASUTG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468744"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF089_03450"
     CDS             complement(<1..236)
                     /locus_tag="VF089_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6173046.1"
                     /translation="MDALELLKQDHAKVKKLFDRTETADDREQKRIFSQIKTELEIHA
                     HIEENIFYPAMQRYDELKELVAESLKEHSGMKTL"
     gene            849..1082
                     /locus_tag="VF089_03455"
     CDS             849..1082
                     /locus_tag="VF089_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173047.1"
                     /translation="MEAHYNGHVIRVSALRVPHTHQWTFTWVVSKQGADPGVKTFTDY
                     GGRFDTADDAISRGYDFAMKWIDDGKPDLFEER"
     gene            1368..2447
                     /locus_tag="VF089_03460"
     CDS             1368..2447
                     /locus_tag="VF089_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173048.1"
                     /translation="MAIFTGELGLQFVTHLANQYTVADLVRLARFAADKGFQQIWVND
                     NIRYRSQLVVLTAIASHVPVRLGTAIMVPYFHNPLDVADSLGALSELCEGREISVGIA
                     RGDLGQSPQHVQPLRPIAMVSETVQLLRQALSREELSYADYPFLQEFYHLNPTGKFRL
                     AFKPRSTFKFYGGGNGPQSLRMCGRIMDGLISSGTYIPMLKAGRLPGMLATADQAARE
                     IDPGKRLRKVCELNVSISTNRAKAIEFPKRQVAHSILQWEALGFTAEEYGKMGVTREQ
                     VLSLKRAFETGATVEEASKLVTEHMVKTYYAAGRPEEVRDQIIKLLGAAERLGYDHVA
                     FAKLGPDYEEAIELLASQVVPALSR"
     gene            2641..>3295
                     /locus_tag="VF089_03465"
     CDS             2641..>3295
                     /locus_tag="VF089_03465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173049.1"
                     /translation="MKNTFLSGLMLCLCLSLLLETSSAAQSKLLSAYISDSPSSSVPA
                     WIAKEAGLFKKYGLDIDLVFIDGSTRGIQSLLAGDLNFTEAVGTSAINGRIAGGDIAI
                     VNGVVNTLPYYIVGKANIKSREDLKGRTAAVHIPGTAADFALRLALKQVGIPYNQIKA
                     ITVGGGPARISSVIAGQTDFTIVSDAEKIQGERSGLKVVIDMAALKVPFLYTCTVTSR
                     "
BASE COUNT          765 a          871 c          871 g          788 t
ORIGIN      
        1 atgagcgttt tcataccact gtgctctttt agcgactcgg cgaccagctc tttaagttca
       61 tcgtacctct gcatggcagg ataaaagatg ttctcctcaa tgtgcgcatg gatctccaac
      121 tcagtcttga tttggctgaa aatgcgtttc tgctctctgt catcagcagt ttcagtcctg
      181 tcgaataatt ttttgacttt cgcatgatcc tgtttcaaaa gttctaatgc atccatttac
      241 cctccttgaa ctcgctcgag cacaattcca ccttcacatt tcacgtctag atacttgcgt
      301 agctatgcag ttgtttatgg gtattcagcg cgtccaggca cgttgcgtaa gtattgttca
      361 gcaaattgag caagccagat gccaataaaa gtggcgccct catggaagga gattcttaca
      421 gcgccgatgg cgacaagcat atgcataacc agatcgtctg cagcggtatg gattgaagcg
      481 gctccagctg tacaacagat gcgcagctgg aacgaagtcc ggttcctgac agtcagtatg
      541 taacgggccg cagcatgtga tgaattcttt ggttatgggg tcagatgaac gttgccatgt
      601 tgtaccaccg ctagaattct acagctcttg taaactcgca tttgggacaa gcaaccgccg
      661 tcagagtctt cttgaactta cctagcagtg acaagtcgct ctagaaaccc ctggccgttt
      721 tgtcgtcgac gcaaacctcg gacttctccg ccgcgggaca gcctatcttt ttctcgctcc
      781 gcggagtgtt agacttcata caaacctgac gagaacgttg taagccgatt tgaccaatgc
      841 ggagagcaat ggaagctcat tacaatggac acgttatccg ggtaagcgcc ttacgggttc
      901 cgcacaccca tcaatggaca tttacatggg tggtttcgaa gcagggcgct gacccaggag
      961 taaagacgtt tactgattat ggcggccgtt tcgacaccgc cgacgatgcc atatcgcgag
     1021 ggtatgattt cgccatgaag tggatcgatg acggcaagcc ggatcttttc gaagagagat
     1081 aaccccagct gagctaaccg gcaggtgagc gccgatgaat agttaatatt ctgggagaag
     1141 agcgtaccgt caggtttcca tccacacagc gcaatgctct cgaattgaaa tccctccccc
     1201 aacttgcctt tcccgggtaa atgcggcttc ggccgttgca accttgaccg aagactctgc
     1261 gattctgctc atcacgtgac gatcaaagtt catgccggtg acctgctctg tttgccgcaa
     1321 gctgcatgag catgccataa atgggctaaa ccaaagatgg agtaagcatg gcaattttca
     1381 ccggagagct cggactgcaa tttgtcacgc atctggccaa ccaatatacg gttgcagatc
     1441 tggtgcgact ggctcgtttc gccgcggaca agggttttca gcagatctgg gtaaacgaca
     1501 atattcgtta ccgcagtcag cttgtcgtac tcacagcgat tgcctcacat gtgccggtcc
     1561 gcctgggcac cgcgatcatg gtgccgtact ttcataatcc gctggatgtc gccgactcgc
     1621 tcggcgccct ctccgagctg tgcgaaggtc gggagataag cgtgggaatt gccaggggcg
     1681 acctcggaca atccccgcag cacgttcagc ctcttcggcc tattgccatg gtcagcgaaa
     1741 cggttcagct gctgcggcag gcattgagcc gcgaagagtt gagctatgct gactatccat
     1801 tccttcagga gttttaccat ctcaatccga caggaaagtt tcgcctggct ttcaaacctc
     1861 gatcaacttt taaattttac ggtggcggca atggcccgca gtcgctgcga atgtgcggcc
     1921 gcatcatgga cggattgatc agctcgggaa cgtatattcc gatgctcaaa gctggacggc
     1981 ttcccggcat gctcgctacc gcggatcagg ccgcacggga aatcgaccct ggaaaacgat
     2041 tgcgcaaagt atgtgaactt aacgtgtcga tctcgaccaa ccgagctaaa gccatcgagt
     2101 ttcccaagcg ccaggtggct cattcaattc tccaatggga agccttgggg ttcaccgccg
     2161 aggagtatgg caaaatgggc gtgacccggg aacaagtgct tagccttaag agagctttcg
     2221 agacgggagc caccgtggag gaagcttcta aactggtcac cgagcacatg gtcaaaacct
     2281 attacgctgc gggcaggccg gaagaagtca gagatcagat tatcaaattg ctcggcgccg
     2341 cggaaaggct cggctacgat catgttgctt ttgccaaact cggcccagat tacgaggaag
     2401 ccatcgaact ccttgccagc caggtggtgc cagcgttatc ccgatgagcc ggctagctcg
     2461 aactgcacga gtattgtggc atgaatttgt ggtaaacatt gctcgcctcc taatggagac
     2521 tggaatgatt ttattgcgct gcccgcacga taattggcag cgttcgacga ctaggcttgg
     2581 gcgattcgca gtaaagcgcg tttttgaacg gtgttcatca tcatttggat cgaggatcga
     2641 atgaaaaata cttttcttag cggcctgatg ttgtgcttgt gtctttcact gctacttgaa
     2701 acaagctcgg cagcgcagag caagctgctg tcggcctaca tctccgattc gccgagttcc
     2761 tcggtgccgg cgtggattgc caaggaagca ggcctgttca agaagtacgg cttggatatc
     2821 gaccttgttt tcatcgacgg cagcacgcgc ggcatacaaa gtctgcttgc cggtgatttg
     2881 aattttaccg aggcggtggg tacttcggcg atcaacggca ggatagccgg cggagatatc
     2941 gctatcgtta acggcgtggt caacactttg ccttactata tcgtcggcaa agccaacatc
     3001 aaatcacgtg aagacttaaa aggcagaacg gcggcggtgc acatccccgg aaccgccgcc
     3061 gattttgccc tccgcctcgc cctgaaacag gtcggcatcc cttataatca gatcaaggcc
     3121 atcaccgtgg gcggcggtcc ggcgcgcatc tcctccgtga tcgccgggca gaccgacttc
     3181 accatcgtca gcgatgccga gaagattcaa ggagaacgta gtggcctcaa ggtggtgatc
     3241 gacatggcag ctctcaaagt gcctttcctt tacacttgca cggtgacgag caggc
//
LOCUS       DASUTG010000153         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1803632, whole genome shotgun sequence.
ACCESSION   DASUTG010000153 DASUTG010000000
VERSION     DASUTG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803632"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..145)
                     /locus_tag="VF089_03470"
                     /pseudo
     CDS             complement(1..145)
                     /locus_tag="VF089_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            289..1083
                     /locus_tag="VF089_03475"
                     /pseudo
     CDS             289..1083
                     /locus_tag="VF089_03475"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1152..1520
                     /locus_tag="VF089_03480"
     CDS             1152..1520
                     /locus_tag="VF089_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6173050.1"
                     /translation="MLAANKRPNTAYVLKESFGQLWDYRTERGARAFFERWQDALKWQ
                     RLEPYRKFARMIERHWEGIASYCDPDNKVGLGLVEGPNNKIRVLQRRAYGYRDEEYLK
                     LKIIAAFLPPLPKFTKNDPH"
     gene            complement(1607..2188)
                     /locus_tag="VF089_03485"
     CDS             complement(1607..2188)
                     /locus_tag="VF089_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173051.1"
                     /translation="MLKDDVGVLIALSADCSQRNHNEMGLLDGYYAHIKPVLLVIAGI
                     AAIGYGVGTQLSSSEEFSEIRGILVRYEIAESESRRSEPHSVILVLRDGRRFWTNAVN
                     KYEAGQLFGMLPARVRVFIERKAPPKLKTGAVKSYGLTVNGQDILTLDAALRREMIIA
                     RYGLPVLGVLAITGGVLLYVIKKQRKGGGKIKF"
BASE COUNT          621 a          826 c          716 g          590 t
ORIGIN      
        1 agagcgccgc gatgatgttg gctgtccgcg cttcccaatc ataaccggcg aggatcgttt
       61 tctcgatttt gagagcatct atcaaagcga tgatatcgag agcgaccacc gactgctggc
      121 cattccggaa cgttccactt gaaagagggt ctacgcggat tagtgtgggt gccaatctgt
      181 ggttccatcg cagtttgtcc ctataattcc agcgttttcg cattctgggg gtagcaagaa
      241 acatgtttcg tctcagttgc aggtttacgg catgaagtcg cctcgcacat tgcgcgagtt
      301 gttctcgctg ccgggcttcg tggccagtgc aacactggag ggtgtttttg gcgaccgcta
      361 tgcacgggtt gtcaccctca ggcggcgaaa aaaaccgccg tctgttctaa ctgcggtcat
      421 tgctgcgcca accgctacga caggcgcacc cagcgggtac gcgatctttc ggtggccgac
      481 tggcggattt acctggcgtt cgggcgctgg cgcgtccact gtccgaggtg cggtggcgtg
      541 cacgtagagc accgcgactg gctggcaaag aacccgcgct atacccagcg ctttgcgctg
      601 catgtgggca agctgtgccg cgacatgccg aacaaggtgg tcgccgagat ggagcgtctg
      661 caccacggca cggtgaagga tctcgacaag ctctacatgc aacagcagct cgagcgcgcc
      721 ggcctgccgg ccccgcgcgc catcggcatc gacgagctct ccattcgcaa ggggcataac
      781 taccgggtca tcgtcagcga cctggagcgg ggccgtccca tctgggtcgg cggcgcgggc
      841 cgcaaggagg ccgacctcga tcggttcttt gccgatttgg ggccaaaaaa ggccgcccgc
      901 atcgaactgg ccgtgatgga tatgtggaaa ccgtttcgca attccgtcac ccgcaacatc
      961 cctaatgccc gtatcgtctg cgacaagttc cacgtcatgc gccacctggc cgacgccctc
     1021 gacgaggtgc gcggaagcga ataccggcgc ctggcgggca aggagcgcag ctacatcaag
     1081 ggcaaccgct atgtgctgct ctccacagcc gagaaaacct ctccctggat ggccgccacg
     1141 cgctcaagaa gctgctcgcc gccaacaaac gacccaacac cgcctacgtg ctcaaagagt
     1201 ccttcgggca actctgggac taccgcaccg aacgcggtgc gcgcgccttc ttcgagcgct
     1261 ggcaggacgc cctcaaatgg cagcggctgg aaccgtaccg caagtttgct cgcatgatcg
     1321 aacgtcattg ggaaggcatt gcctcctact gtgatcctga caacaaggtc ggcctcggtt
     1381 tggtcgaggg tcccaacaac aagatccgtg tcctgcagcg acgggcgtac ggctaccgcg
     1441 atgaggagta tctcaaactc aaaatcatcg ccgccttctt gccgccactg ccgaaattca
     1501 cgaaaaatga cccacactaa tccgcgtaga ccctcttttc ttacttcaca ccaaattgac
     1561 gatatcgcga acggttttgg cgaccagagc aaggggggcg aagtgattaa aatttaatct
     1621 tcccaccccc tttgcgttgc ttctttatca catacaggag cacccctccc gtaattgcga
     1681 gaacgccgag cacaggaaga ccgtaccgag cgattatcat ttctcgcctc agcgccgcat
     1741 ccaacgtgag aatatcctgc ccgttcacag tcaaaccgta tgactttaca gccccggttt
     1801 ttagttttgg aggtgctttt cgctcgataa acacacgaac ccgggcgggc aacataccaa
     1861 acaactggcc agcctcatac ttgtttactg cattagtcca gaagcgccgc ccatccctta
     1921 gcacgagtat tacggagtga ggctccgaac ggcgcgactc actttcagcg atctcgtacc
     1981 ttacgagaat cccgcgtatc tcagaaaact cctcgctgct tgaaagctgc gtaccgacac
     2041 cataaccaat ggcggctatg cctgcgataa ccaataatac tggtttgatg tgcgcgtaat
     2101 atccatcaag gagtcccatt tcgttgtgat tcctttgtga gcaatccgca gatagcgcaa
     2161 taagtacacc aacatcatcc ttcagcaagc gtagcctgga tgaagcgaag cggaatccag
     2221 gaaaccactc gccggcattt atcccattcg attccgcgat gccgagagat agcgaagacc
     2281 ctatgtccaa tatttctacg gtacgatagt aaccgaagat atttccgagt atccgttaag
     2341 ctcgcatttc ccggtcacaa aaacacggac cttctttcct gatgctactg cctccaatag
     2401 cgccgcgaac atctcctttg acccagaagc actataatcc tgataaaaca cctccttgtt
     2461 tctgcaaccg ctgggatttt cagagacctt caatttgacc aggaagcgcc catggatggt
     2521 cggtgtgagc tccgctaccg acgcataatc tgtccacccc gacacggcaa ctgccgtcat
     2581 cggtaccaag gcgtgcagta catctcaatc ttattttcat gtaagtactc catggaggat
     2641 tattggagcc cgtgtaggct ctgagcgctc cccacgaaat tgcgtttaaa attggctgtt
     2701 tattgaaccc gagccagcgg aaggggaacc tgcacgacac ggcgcgatcc gat
//
LOCUS       DASUTG010000154         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1506871, whole genome shotgun sequence.
ACCESSION   DASUTG010000154 DASUTG010000000
VERSION     DASUTG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506871"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VF089_03490"
     CDS             <1..446
                     /locus_tag="VF089_03490"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX6173052.1"
                     /translation="NTLLLNKRRELRQGDVLYSVICNPMMTITGYSNEESYAYGPMCS
                     EELNRIRYGVPGTRNCRDNFAMALAPHGFDRRQIPNAFVPFMRVEVEANGSMEIKEPT
                     SRAGDFYDLRAEMDLLVAVSNCPQERNPCNGFNPTPMGMIIYEPG"
     gene            481..1521
                     /locus_tag="VF089_03495"
     CDS             481..1521
                     /locus_tag="VF089_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173053.1"
                     /translation="MMKLIGLAQLQLKTTSLLALLSVIGNLSFSPADLRAAEKAKETP
                     VTIRLDFIVGGNHAPWFVAWEKGFYAKRGLNVTIQPGTGSADTIRTIAAGGADVGFAN
                     VSTAIVGRSRGAPIVSVAQLGYIAVTVLWRDETDIKSLKDLERKSWAISPGQAQWFLL
                     PALARINNLDFKSIKIEETAPPLQPAALLAKKADFITMFRASNDEVAEMTAAKQGIRL
                     KRIHMRDAGLDIYGSALVVKEEDIKRRPEMIRAYVEGTMEGLRYTRDHQEEALKILLK
                     LKPELDKELTRLQIKNGVEEVFIPAESLASGYGYMKPDVMEKTIKITNEFFDVAGKVS
                     TAGVYTNQFIGK"
     gene            complement(1551..2489)
                     /locus_tag="VF089_03500"
     CDS             complement(1551..2489)
                     /locus_tag="VF089_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173054.1"
                     /translation="MAGLDHLRLLQFRAGYNLPVHAAVEHGIFAKHDLEVEVAYTPGS
                     DYLIEALQTGKCEIAHPAADDLVAAVESEPYKHDSEIDLFLFMGLHSGLLSLIAAPEY
                     PDVESLRGKLLGVDSRRTGFVFLLEKFLRSKGFGLADYSLVEIGGWEFRYRALLNKQI
                     AATLLTSPFVADALASGCHLLAGGDEILPVYQATCGAARKAWARDHNEVLVRYIRAYL
                     TATRWCFNPSNRRACLDLLKKHSGLDEERAENTLATLLEPDRGLYPDAKLNIPGLAAV
                     LTLRAEMGYLQPPLPPAEKYIDLSYYRRAAGVAEAS"
     gene            2641..2928
                     /locus_tag="VF089_03505"
     CDS             2641..2928
                     /locus_tag="VF089_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173055.1"
                     /translation="MKKPAGDSALFPSPSTGRTKVGVMALIGPEHPYPNLSPSRGKEI
                     QIGCGDMLPFPFSGGQRKIMNHFEEKPRNCIDRAVTTPLCGISNRKSQRQN"
     gene            3201..3986
                     /locus_tag="VF089_03510"
     CDS             3201..3986
                     /locus_tag="VF089_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6173056.1"
                     /translation="MENRPSNGTPSIKWSALFFLSILVLWQLILPLLSIPSYLLPTPL
                     EILAEFQKRGGFIFGHFLPTFYETMLGFLIGALVGVVSALGIVYSRILWITIYPSLVV
                     IGSVPRIAIAPLILIWLGIDSIWSKVTIAFLVAFFPMVVNSVVGFSQIEGDMLDLAKT
                     MGMKPWTEFRKIRLPNSIPYLFAGFKASIALAVIGAVVAEFVSGQQGLGYVIIIGNNN
                     LNAALIFAAITLLSIMSLGLFGIIVGLEKVLMPWRRGIYPTEL"
BASE COUNT          948 a         1150 c         1090 g          900 t
ORIGIN      
        1 gcaacactct gctactgaat aagcggcgcg agctgcgcca gggagacgta ctctattcgg
       61 tcatctgcaa tccgatgatg accatcactg gctactccaa cgaagaaagc tacgcctacg
      121 gccccatgtg cagcgaggag ctgaaccgca tccgctacgg cgtgcccggg acgcgcaact
      181 gccgtgacaa cttcgccatg gcgctcgctc cccacgggtt cgaccggcga caaattccca
      241 acgccttcgt cccgttcatg cgggtggagg tcgaggctaa cggatcgatg gaaatcaaag
      301 aaccgaccag ccgcgcgggc gacttctacg acctcagggc agaaatggac ctgctggtgg
      361 cagtgtctaa ctgtccacag gagcgcaacc cctgcaacgg tttcaatccc acgccgatgg
      421 gcatgatcat ctatgaaccc ggttagaggc aatgtgccgc ataccctatg ttaggagacc
      481 atgatgaaac tcatcgggct ggcgcagctg cagctaaaga caacctcttt actggccttg
      541 cttagtgtca ttggcaacct gagtttctcc cccgccgatc tgagagccgc tgagaaagcc
      601 aaagagactc cggttaccat ccggctcgat tttatcgtcg gaggaaatca tgcgccgtgg
      661 tttgttgcct gggagaaagg cttttacgcc aagcgcggcc tgaacgtcac cattcagccc
      721 ggaacaggtt cagcggatac gatccgcacc atcgctgcgg gcggggctga cgtgggtttc
      781 gccaacgttt caactgccat agtcggccga tcccgaggcg cgcctattgt ctcggttgcc
      841 cagctcggct acatagccgt cacggttctc tggcgcgacg aaaccgacat taagtctctc
      901 aaagatctgg agagaaaatc ttgggccatc agccctggac aggcgcaatg gtttctgctg
      961 ccggcgcttg ccaggatcaa caacttagac tttaagtcga tcaagatcga ggagaccgcg
     1021 ccgcctcttc agccggcggc tttgctggcc aagaaagccg acttcatcac gatgttccgc
     1081 gcgtcgaacg atgaagtggc cgagatgact gcggctaaac aaggcattcg attaaaacga
     1141 atccacatgc gcgatgccgg gctcgatatc tacggcagcg ctctcgtagt caaggaagag
     1201 gatatcaagc gccggcccga aatgattcgc gcctacgtcg agggaaccat ggagggtcta
     1261 agatacactc gcgatcacca ggaagaggcg ctgaaaatct tgttgaagct caaaccagag
     1321 ctggataagg agctgacaag actgcaaatc aaaaacggcg tggaggaagt attcattccg
     1381 gcggaatcgc tggcgtcggg atacggttac atgaagcctg atgtgatgga aaagactatc
     1441 aagatcacca acgagttttt tgacgtcgcc ggaaaggtct caaccgccgg tgtttacacc
     1501 aatcagttta tcggaaagta agcacgcgcg gcgccatgcc ggcgtcgcga tcaactcgct
     1561 tccgcgacgc cggccgcgcg ccgataataa gagagatcga tgtatttctc cgccggcggt
     1621 aaaggcggct gcagatagcc catctcagcc cgtaacgtca ggacagcggc gagcccggga
     1681 atattgagct tagcatcggg gtagagacct cggtcaggct ctaaaagcgt ggctaatgta
     1741 ttttccgcac gctcctcgtc gaggccgcta tgttttttga gcagatccaa acaagcgcgg
     1801 cgattcgacg gattgaaaca ccagcgcgtc gctgtcagat aagctctgat gtatcgcact
     1861 aagacctcgt tgtgatctct cgcccaggcc tttctcgcgg cgccgcaggt cgcttgatag
     1921 acaggtagaa tctcgtcgcc gccggccaag agatgacagc cggatgccaa agcatccgcc
     1981 acaaaaggcg aggtcagcaa agtcgccgcg atttgcttat tcagcagagc gcggtagcgg
     2041 aactcccaac caccgatctc gaccaggctg taatccgcga gtccgaatcc cttagaccga
     2101 agaaactttt ccaacaagaa gacgaaaccc gtgcgtctgg aatccacgcc aagtaatttg
     2161 ccccgaagag actcgacgtc gggatactcc ggcgcagcaa tcaggcttaa gagaccgcta
     2221 tggagtccca taaagagaaa tagatcgatt tcgctatcgt gcttataggg ttcgctttct
     2281 accgccgcca caagatcatc ggctgccggg tgggctattt cgcacttgcc ggtctggagc
     2341 gcctcgatga gataatcgga accaggagtg taggcgactt caacttcaag atcatgcttg
     2401 gcaaagatgc cgtgctctac cgccgcgtga accggcagat tatagccggc gcgaaactga
     2461 agcagtcgca gatggtccaa tcctgccatt gaatcctccg tcctttaacc gcgttttaca
     2521 gtgccggcaa aatatcacga acgtgaccgg cactggaaca cttaatcgaa cctgaagtct
     2581 agctggatta ttcatgcttc gagaaagttg ggtttcgggg accgcgcctt tgcctgactt
     2641 atgaagaagc cggccggcga cagcgcgctg tttccttccc cctcgacggg aaggacaaag
     2701 gtaggggtga tggctttgat cggtcccgag cacccctatc ccaacctttc cccgtcaagg
     2761 ggaaaggaga tccagatagg atgcggcgat atgctgccat ttccattctc cggaggccag
     2821 cgtaagatca tgaatcactt cgaggaaaaa ccacggaatt gtatcgatcg agcggtgacc
     2881 acgccgctct gcggcatctc aaatcgcaaa tctcaacggc agaactgaga tctggcatct
     2941 gaaatctgag attcccagcg cagcgaggac taggtccatc ggccgcgact ggagaggatc
     3001 gttcctcggg cgctctttgt gaaaaactcg accttgccgc aatccgcttt gtccttgaca
     3061 caagtagtcc ctttcgataa tcaaaacccc agcgtaggtc cgtatcggta cgagaccgtt
     3121 cgtcggccga gcgcacagcg aaaggcaaaa agtttgtaac gatatttccg ccggctcctt
     3181 gaaatcgctt accggtaaaa atggagaacc gtccgtctaa cggcacgcct tccattaaat
     3241 ggtcggcgct atttttcctc tctattctag ttctctggca gttgattctc ccgctgctga
     3301 gtattccgtc atatctactt ccgactccgt tggaaatttt ggccgaattt caaaagcgcg
     3361 gcggttttat tttcgggcac tttctaccaa ctttttacga gaccatgcta ggttttttga
     3421 tcggcgcgtt ggtcggcgtc gtctccgctc tggggatagt ctactcgaga atactctgga
     3481 tcacgatcta tccatcgctg gtcgtcattg ggtcagtgcc gcgaattgcc atcgctccac
     3541 tcattttgat ttggctcggc atcgactcca tttggtccaa ggtcaccatt gcttttctcg
     3601 tggccttctt ccccatggtg gtcaattccg tcgtgggatt ttcacagatc gaaggcgata
     3661 tgttggacct ggccaagacc atgggaatga aaccttggac tgagttcagg aagattcgtc
     3721 tgccaaactc gattccttat ctatttgctg gcttcaaagc ctccattgct ctagcggtga
     3781 tcggcgccgt ggtcgccgag ttcgtctcag gccaacaagg tctcggctat gtgatcatca
     3841 tcggcaacaa caacctcaat gccgcgctca tattcgccgc catcacgctt ctttccatca
     3901 tgtccctggg actcttcgga atcatcgtcg gtctggaaaa ggtgctcatg ccctggcgtc
     3961 gcggcatata tcccacagaa ctttgagcgg catgctcgcg cccggaatcg agaacctgaa
     4021 caggaggaca atcatgaaaa cacaattgat acacctcgga gcggtgcttt tgctcagcgg
     4081 cggtcttg
//
LOCUS       DASUTG010000155         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_24740, whole genome shotgun sequence.
ACCESSION   DASUTG010000155 DASUTG010000000
VERSION     DASUTG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24740"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VF089_03515"
     CDS             <1..990
                     /locus_tag="VF089_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX6173057.1"
                     /translation="ASLAVEGIRVRLSGQDSARGTFSHRHAVLRDYHDGHPYVPLQHL
                     QRGQAPVEIINSPLSETGVLGFEYGYSLDYPEGLILWEAQFGDFVNAAQVIIDQFITS
                     AEEKWRRLSGLVLLLPHGFEGMGPEHSSARIERFLQLCAKDNIQVVYPTTPGQYFHCL
                     RRQVLRKWRKPLVIMTPKSLLRHPGAVSTLDALADGEFESVIADSSAQENYQKVLLCS
                     GKVYYDLEKKRAELNRTDVAIVRIEQFYPVPEERLRSALERYPSGTPVVWIQEEPRNM
                     GAACFWHLQLGNKLFNRFPFSVVARDASASPATGSASRHHQQQTELLAAAFGQ"
     gene            1040..2332
                     /gene="odhB"
                     /locus_tag="VF089_03520"
     CDS             1040..2332
                     /gene="odhB"
                     /locus_tag="VF089_03520"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX6173058.1"
                     /translation="MTIEVKVPEVGESITEVEIGDWLKAVGDSVQQDEDLVVIETDKA
                     TVEIIAPQAGRLTKILKASGETAQVGEVIGHLDQVDASSTDQAREDQTGIAEPPQAKK
                     PKGRRGKVNKPEQPVSARESDFDDVPDAQPSATDSTAGLIEQPAQENQINAAARPSQA
                     AELEIEAKPSFTDPGLPSERTPPAQTPTPSGAREPRGEREEKVVPMSQLRRRLAERLV
                     QAQQTAALLTTFNEIDMTAVNQLRQMHQDAFKEKYNVRLGLMSFFVRAAVEALKLIPE
                     ANAEIRDGSIVYRNYCDIGVAVGGGRGLVVPVLRDAQSMSFAAIEIAIGDFARRAREN
                     KLKPDELQGGTFTISNGGVYGSLLSTPIINPPQSAILGMHAMQERPVARDGAVIIRPM
                     MYVALSYDHRIIDGREAVTFLKQIKTSLEEPVRMLLEV"
     gene            2351..2548
                     /locus_tag="VF089_03525"
     CDS             2351..2548
                     /locus_tag="VF089_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173059.1"
                     /translation="MAELRSIDILQRRENLDRQNFRQRVAGLGQCKLRMGIGLDPEKL
                     PAIVMYAHKRNIVCRATGPKC"
     gene            2563..>3021
                     /locus_tag="VF089_03530"
     CDS             2563..>3021
                     /locus_tag="VF089_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX6173060.1"
                     /translation="MAQIFHPSTNTISKLTIFGAVFLIGGLLWLLAELNRSDYVTQAN
                     VVRPQPVPFSHKHHVSGLGIDCRYCHTAVEQSAFAGIPSTSTCMNCHSQIWTNSPMLE
                     PVRESYRTGQPLRWTRVHNLAEFAYFNHSIHVNKGVGCATCHGPVDQMPLV"
BASE COUNT          703 a          834 c          861 g          623 t
ORIGIN      
        1 gcgagtctag ccgtcgaagg aatcagggtg cgcttatccg gccaagacag cgcgcgcgga
       61 accttcagcc atcgtcatgc agtgctgcgt gattaccacg acgggcatcc ctatgttcca
      121 ctgcagcacc tgcagcgggg gcaagctccg gtggagatca tcaatagccc tctttccgaa
      181 accggcgttt tgggtttcga atacggttac agccttgatt atcccgaggg cctgattcta
      241 tgggaagcgc agttcggcga tttcgtcaat gctgctcagg tcatcattga ccaattcatt
      301 accagcgccg aagagaaatg gcgcaggcta agcggcttgg tgttgttgct cccgcatggc
      361 tttgaaggca tgggacctga gcactcgagc gcgcgcatcg aacgctttct gcagctttgc
      421 gccaaggata acatccaggt ggtttacccc acgacaccgg ggcagtattt tcattgcctg
      481 cgtcgacaag tgctgcgcaa atggcgtaag ccgctggtga ttatgactcc caagagttta
      541 ctgcggcatc ccggggcagt gtccactctt gatgccttgg ctgatggaga gtttgagagc
      601 gttatcgccg atagtagcgc ccaggaaaat tatcagaaag tcctcttgtg cagcggcaaa
      661 gtttattatg acctggagaa aaagcgcgcc gagttgaacc gcacggacgt cgccattgtg
      721 cgcatcgagc agttctatcc ggtgccggaa gagcggctgc gctctgctct ggaacgctat
      781 cccagcggaa cgccggtagt atggattcaa gaagagccgc gtaatatggg cgccgcatgc
      841 ttttggcacc tacaactcgg caacaagcta ttcaatcgct ttcccttttc ggtggtggcg
      901 cgcgacgctt ccgccagtcc ggcaacgggc tcggcaagcc ggcatcatca acaacaaact
      961 gagctgttgg ccgccgcttt cggacagtag cgggacgccg agcttacact gatgctgtat
     1021 aggactacta aggtcggcaa tgaccattga ggttaaagtt cccgaggtcg gcgaatccat
     1081 caccgaggtc gaaattggtg attggctcaa agccgtcggc gactcggttc agcaggatga
     1141 ggatcttgtc gtcattgaaa cggataaggc aacggttgag attattgcac cgcaagcagg
     1201 ccggctcacc aaaattttaa aagccagcgg tgaaacggcg caagtcggcg aagtcatcgg
     1261 ccatctggac caggttgacg ctagcagcac tgatcaagct cgagaagatc agaccggtat
     1321 cgctgaaccg ccccaggcca aaaagccaaa aggccgccga ggcaaagtga ataagccgga
     1381 acaacccgtc agcgcgcgcg aatccgattt cgatgatgtt cctgatgcgc aacccagtgc
     1441 gaccgattcc actgcaggtc tgatcgagca accggcgcaa gaaaaccaaa taaatgccgc
     1501 ggcgcgaccc agccaagccg ccgaactcga gatagaggca aaaccgtcgt ttaccgatcc
     1561 tggactgcca tccgagcgga cgccgcctgc ccaaactcca acaccctcgg gtgccaggga
     1621 gccccggggg gagcgagaag aaaaagtcgt gccgatgagc cagttacgtc gccgactggc
     1681 cgaacggtta gttcaggcgc agcaaactgc cgcgctgctg acgactttca acgagatcga
     1741 catgaccgca gtcaatcagc tgcggcaaat gcatcaagat gctttcaagg agaaatataa
     1801 cgtcaggctc ggcttgatgt ctttctttgt tcgcgctgcc gtcgaagcgc tcaagcttat
     1861 tccggaagcc aacgcagaga tacgcgacgg cagtatcgta taccggaact actgcgacat
     1921 tggtgtcgcc gtgggtggcg gacgtgggct ggtcgtgccg gtgctacgcg atgctcagag
     1981 catgagcttt gcggccatcg aaattgccat cggcgacttt gcccgccgcg cccgagaaaa
     2041 caagctcaag cctgacgagt tgcaaggcgg gacttttacg atcagcaacg gcggcgtgta
     2101 cggatcgcta ctgtcaacac cgatcatcaa cccaccgcag agcgccatcc tggggatgca
     2161 cgcgatgcaa gagcggccgg tggcgcgtga cggagcggtc ataatccggc cgatgatgta
     2221 tgtcgctttg agctatgatc accgaatcat cgacgggcgc gaagcagtca cgtttctgaa
     2281 acagataaaa acatcgttag aggagccggt gcgaatgtta ctggaagtct aatcaatcgg
     2341 ggatccagta gtggctgaat tacgatctat agacatactc cagcgtcgag aaaatctcga
     2401 caggcagaat tttcgccagc gcgtcgctgg tctcggtcaa tgcaaactac gcatgggcat
     2461 tggccttgat cctgaaaagc ttcccgcaat cgtcatgtac gctcacaagc gtaatatcgt
     2521 ctgccgggca accgggccta aatgttaatc aagcggcagt gaatggctca aatatttcat
     2581 cctagtacca acacgatctc caagttaact atctttggcg ccgtgttcct gatcggcggg
     2641 ctcttatggt tattagccga gctgaaccgt tcggattatg tcactcaggc taacgtcgtg
     2701 cgcccgcaac cggtgccgtt tagtcacaag catcatgtca gcgggctggg catcgattgc
     2761 cggtattgcc ataccgccgt ggagcaaagc gcctttgccg ggattccctc gacgtcgacc
     2821 tgcatgaatt gtcactcgca gatctggacc aacagtccga tgctcgaacc ggtgcgcgaa
     2881 agctaccgca cgggtcaacc cctgcgctgg acccgggttc ataacctggc ggagtttgcc
     2941 tatttcaatc acagcattca tgtgaacaag ggcgtcggct gcgcgacttg tcatgggccg
     3001 gtggatcaga tgccgctggt t
//
LOCUS       DASUTG010000156         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1062105, whole genome shotgun sequence.
ACCESSION   DASUTG010000156 DASUTG010000000
VERSION     DASUTG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062105"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF089_03535"
     CDS             <1..283
                     /locus_tag="VF089_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173061.1"
                     /translation="ESGSHLRRLLADIRKRQLDFADCYSATAENSGSKLAQRPGATKW
                     ALPLGSSRGMMTIARPTDSISFVGFGSRTVQRPFAVYQLNALAEFTPRL"
     gene            complement(348..986)
                     /locus_tag="VF089_03540"
     CDS             complement(348..986)
                     /locus_tag="VF089_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014925376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEX6173062.1"
                     /translation="MSPSIDATAISRAIHFGTATLHEAMGRKGALPHRIKPISPRMKV
                     AGPALTVSSPPMDNLMLHQAIYAAEPGSIIVAEVNRGYEGGYWGEIMTIAAQQRKISG
                     LVLDGCVRDADLIEQLGFPVFSRGLSVRGTDKQGGGHINWPILLGDITVNPGDLVVGD
                     RDGVIVIPAAEISSTLEAAQKREAKEDQTKRDLMAGKSTLGIYGWPPAKLNS"
     gene            complement(1045..1716)
                     /locus_tag="VF089_03545"
     CDS             complement(1045..1716)
                     /locus_tag="VF089_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX6173063.1"
                     /translation="MEREAIIERLAKLDTCSVSDALDGLALSGATWGVRPQWHCPRIV
                     GRAVTVKIKPIGLQKPTQHLGAPAIDAAQPGDVIVIDNAGQRQFSTWGGLLSLSAKLK
                     GLSGVVIDGACRDIDEARDLEFPIYARGVVPMTARGRVMQDSFNQEIQFAGVQVHPGD
                     LVIADGSGVVIIPREKEEAVVTAAELVSRKEAEMAAGIRQGVSGLEMLEKLGYEGMLQ
                     SGRAK"
     gene            2286..2564
                     /locus_tag="VF089_03550"
     CDS             2286..2564
                     /locus_tag="VF089_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173064.1"
                     /translation="MSVNTPYRGLLRLLTARDPALFRGMVIADVALSVSHAVFVYKIS
                     NCAGQSVLFRVAAKRMDDDGSAVVGRSRLQHHFRCTSSIGIDKRSVAG"
     gene            2569..3066
                     /locus_tag="VF089_03555"
     CDS             2569..3066
                     /locus_tag="VF089_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016976221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6173065.1"
                     /translation="MSENPPLHEPQPNIPSFESPPAAITPLSPETQETSLAPTLPTRA
                     PSTMIETGLASWYGARHHGKRTASGEIFDQNKFTAAHRTLPWGSIVKVTNLDNEKSVE
                     VRINDRGPFKKGRIIDLSRAAARALGMVRSGVSPVRMELLSPREIASDLVLQNSQSDR
                     ATDSN"
BASE COUNT          745 a         1021 c          910 g          767 t
ORIGIN      
        1 ggaatccgga tcgcatctgc gccggttgct ggcagacatt cgcaagcggc aattagattt
       61 tgctgattgt tactccgcaa ccgcggagaa ctcgggatca aagcttgccc aacgtccggg
      121 cgcaactaaa tgggccctac cgcttggatc gagtcgtggg atgatgacga tcgctcggcc
      181 taccgattcc atttcattcg tcggcttcgg cagccgcaca gtgcaaagac cgtttgccgt
      241 ttaccagctg aacgctctcg cggaattcac cccacggctc tgacggctcg gtgccgatag
      301 cgcgagggtt attgcgaggc gaccggtatt ttcctaagaa ggcagtctta ggaattcagc
      361 ttagccggcg gccatccgta gatccctaac gtcgactttc ccgccatgag atccctcttg
      421 gtttgatctt ctttggcttc gcgcttctgc gccgcttcaa gggttgagga gatctcggct
      481 gccgggatga cgatcacgcc gtctcgatcg cccaccacca aatcgcccgg gttcacggtg
      541 atgtcgccga gcaggatcgg ccagttgatg tggccgccgc cctgcttgtc cgtgccgcgc
      601 accgataagc cgcgcgaaaa caccggaaag ccaagttgct cgatcaagtc tgcgtcgcgc
      661 acgcaaccgt cgagcaccag acccgagatc tttcgttgct gcgctgcaat cgtcatgatc
      721 tcgccccagt aaccgccttc gtaaccgcga ttgacctccg cgacgataat cgatccaggc
      781 tcagcggcat agatcgcctg gtgcagcatg agattgtcca ttggtgggga actgaccgtc
      841 agcgccggtc cagcaacctt catccgcggc gaaatgggtt tgatgcgatg gggcaaagca
      901 cccttcctgc ccatcgcctc gtgtaatgtt gccgtgccaa agtgaatcgc acgggaaata
      961 gccgttgcat ctattgaagg actcataaag ttttccgctt atttctttct tgttattatt
     1021 gttccgtcac cgggttgatg cctgttactt cgctctacct gactggagca ttccctcgta
     1081 gccgagcttc tccagcatct caagaccgga aacgccttgg cgaattcccg ccgccatctc
     1141 ggcttccttg cgagaaacca actccgccgc ggtgactacc gcttcttctt tttccctggg
     1201 aataatgacc acaccgctcc cgtccgcaat cactaggtcg ccgggatgaa cctgaacacc
     1261 ggcgaattga atctcctgat tgaaggaatc ctgcatcact cgcccgcgcg ccgtcatagg
     1321 cacgacaccg cgcgcataga tgggaaactc cagatcgcgt gcctcgtcga tgtctcggca
     1381 agcgccgtcg atcaccacgc cgctgagacc tttcaacttg gccgataacg acaacaatcc
     1441 accccacgtc gagaattgcc gctgtccggc attgtcgata acgatgacat cgcctggctg
     1501 cgcggcgtcg atagccggcg cgccgagatg ctgcgtcggc ttttgcaatc cgataggctt
     1561 gattttcacc gttacggcgc ggccgacgat gcgtgggcag tgccactgcg gccgcactcc
     1621 ccaggtcgcg ccgctcaatg ccaggccatc cagcgcatcg gataccgagc aggtatccaa
     1681 tttcgccagc cgttcgatga tcgcttctcg ttccacttat ttcactgagc aggttgtccg
     1741 atgtggagct taggcgaccg gcagctccag atatccatag cgggtttgcc ttttccgcag
     1801 gctcacttgt ctacgaaagc tctatctgga aagctcggag tctgtcaagc cggagcgagc
     1861 gatcagacca ggcatgtcta tcgtgtttga ccgatggaaa tcacattctc ttcccgcaat
     1921 atggaagttc ttgccagcta cgatttcgta acacagatcc cgtcggtgtt tcgctgtgaa
     1981 aggtcgctga tccgataaaa atattccgtt gaatgattgg ggtagaattc gacatgccta
     2041 ggaaaaagct tggaggtctc tatgtccaca gccaagttac gcttatctgg gcagcgccag
     2101 caacatggca ccgacgccgc ttctcttcat ctcatgagga tacaatgccg atagcgcgtc
     2161 ccgacaacgt acgaaatccc gcccgatcca ctccggcgta gcaaactctc gaaattcttt
     2221 gcctggctga aaaccatcac tttcgtacgc tgtcgtacga acccacccga tttcggctgc
     2281 catttttgtc agtcaatacg ccctatcgcg ggcttttgcg cctcctcacc gcccgtgatc
     2341 ctgcgctatt tcggggcatg gtcattgcgg acgtagccct ttcggtgagc catgcagtgt
     2401 tcgtttacaa gatcagcaat tgcgccggcc aaagtgtcct gtttcgagtt gcggctaagc
     2461 gcatggatga tgacggcagc gctgttgtcg gccgctccag gctgcagcac catttcagat
     2521 gtacctcatc catcggtatc gacaaacgca gtgttgccgg ctgagaattt gagcgagaat
     2581 cctccgcttc acgagccaca gcccaacatt ccctcgtttg agagtccacc tgcggcaatc
     2641 actccgctct ctcccgaaac gcaggaaact tcgctcgcgc caactctccc cacgcgtgcg
     2701 ccatcgacga tgatagagac cgggctcgcg tcctggtatg gagcaagaca tcatggaaag
     2761 cggacagcga gcggagaaat ttttgatcag aacaaattca ccgccgcgca tcgaactctt
     2821 ccgtggggca gcatcgtaaa ggtaaccaat ctcgacaatg aaaagtcggt ggaagtgcgg
     2881 ataaacgatc gcgggccatt taagaaaggc cggatcatcg acctatcgcg cgccgcggca
     2941 agggctctcg gcatggtgcg atcgggagta agcccggtgc gaatggaatt gctttcgcct
     3001 cgcgagatag caagcgacct ggtgttgcaa aacagccaat cggatcgggc aacagacagt
     3061 aattagaacc ccgggttttt acgatcattc aggcatagct aatccaagtc atctacagag
     3121 ccctcggaac cgcggaggtt ttgcttccgt tcactgcttc attcgacacg gcagatcacc
     3181 cgataatcgg acagcgatcg gacagcggcg ggctagctca atctgacccg tgtggttacg
     3241 gtatgcgcgg tcattgattt cacatcacct aacggcgatc aaggcgcgcc agggaagaat
     3301 gcgcgactgt tgttcactga tgccgaagta atttagaaaa gcccaccggt acgaatagca
     3361 actccggcca ggagaaaacc gacagagatc aacgtgacga ggacgatatg ggatgcaagc
     3421 acatgcatgt tatcgcggtt gat
//
LOCUS       DASUTG010000157         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1655104, whole genome shotgun sequence.
ACCESSION   DASUTG010000157 DASUTG010000000
VERSION     DASUTG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655104"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1266)
                     /locus_tag="VF089_03560"
     CDS             complement(289..1266)
                     /locus_tag="VF089_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173066.1"
                     /translation="MSVKFVPLFFCIVICFGTSARVGHGQETVNVALTSKAFQYTILP
                     IAQDRGYLKEEGIDLKLVYMQNAPGLQALTAGNVQFSGSGSSALVAISKGGAPFKTVI
                     AANDQVLQWVVARPNISSIKDLKGKKVGTTGVASIAAFMFRNIMGKYGMDGNKDVVFV
                     DPGPVNRLPSLLSGAVDAAILSPEERYAAIDQGMKDLMFIGKEVKNSWGTIATSDRFI
                     KEQPKVMAGFARAVVKALRYVRHNREGAIAAASKFTELDNALVIRMYDDIAKTFTVNG
                     VVDEEAQRNDLAIVREIAGVTEVVPIQRAYDFSFARQADQQLTKAGWKP"
     gene            1482..2453
                     /locus_tag="VF089_03565"
     CDS             1482..2453
                     /locus_tag="VF089_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6173067.1"
                     /translation="MKAIRIHNFGPTEDVLQFEEVPVPEPGSGELLIKVEAASLNRAD
                     LGLRSGNYRIAPEDLPIIPGREFAGRVTKLGDSVNEFQVGQRVVAYTGTGGYAEYAKA
                     KTSEVRPLPDGVDAAAAAAIPTVFLTAWFGLMEEGKLKSGEWLLVQAGSSGVGVAAIQ
                     IGKHAGAKVATTTSGDEKARRLRQLGADAVIDYTRIDFVAEGMRATGNRGFDVVLEMI
                     GGEVYQKSIEVLAPAGRLISIGGAFGQIPDTPPALADGRTARRFSITNYLKAHPEGFK
                     QLDAALQWVRDGKYQVVIDRSFPLAETRSAQRLLEGRDHLGKIVLVP"
     gene            complement(2475..3023)
                     /locus_tag="VF089_03570"
     CDS             complement(2475..3023)
                     /locus_tag="VF089_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173068.1"
                     /translation="MNDERELGWLRLTDKSQLVLTTGHVFQHQDYGMFGENRAIIPRR
                     AITTVRLSWQRSRWALLFGLILFLASLALIIANQMNQPEGIEFNTRLFAISPATLSLI
                     QYGLLVAGLALIALFWFHKRNEIQILGGTGSIGGTPVNYEDAEEFCLLLMGASKEPAP
                     AVANVEPEPENSPKTTDPDWRL"
     gene            complement(3131..4183)
                     /locus_tag="VF089_03575"
     CDS             complement(3131..4183)
                     /locus_tag="VF089_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173069.1"
                     /translation="MTKVKGFIDADGHVVENDRALLDFLPQPYKGREDLLSFPFFPTL
                     DGFHRQALRVVDGKGRYVAQGSLKEWKEFLDEDDIEWSVLYPTAGLTFGLIRDKDWAC
                     AIAQGYNNYLSELFLKQEKRLRGMALIPLQSIPEAVKELRRAVKELNMVGAILPAVGM
                     RQPFGDEKYFPVYEAAQELDALLAVHAAPTQGIGVDAFDRLIEVRTLSHGFGQMIQMT
                     GMIYGGVFDKFPRLKVAFAEAGCGWVPYLMERMDLEYEHRSAQVPELKVAPSEHLKSG
                     RIFIHCELEELGIPRIVELLRDDILFCATDFPHEPRSEFRENVSKFVAREDLAMETKQ
                     KIMTQNPKRMYPLAAN"
BASE COUNT         1054 a         1223 c         1192 g         1037 t
ORIGIN      
        1 gaacgctgca gcatatatca gcggaacgcg taactggcgg agaaaagatt tagatctact
       61 gaatcgatgt cttagcggac tgctactccg cccagaaatg atgcagtaaa ccagagataa
      121 aaacttagct gcatacggaa ctgcggattg gaaggaggag cgcagacacg gcactcgcgg
      181 ttggatccat gatgatgctc atccaccgtc aatgcagctt gacggcgcga cacggctgat
      241 cagcttacgt cctccgatcg acggcaacgg cttgcgacag gatttgcact acggtttcca
      301 cccggccttg gtcaattgct ggtctgcttg gcgggcgaag ctaaagtcgt aggctcgctg
      361 aatcggcacg acctcagtca cgcccgctat ttcccgaacg atagcgaggt cattgcgttg
      421 cgcctcttcg tcgacgactc cattgacggt aaaggttttt gcgatatcgt cgtacatgcg
      481 tatgactaaa gcgttatcga gctcggtaaa tttacttgcc gcggcaatcg cgccttcacg
      541 attgtgccgc acataacgta atgctttgac gacggcgcgg gcaaaaccgg ccattacctt
      601 cggctgttct ttgataaaac gatccgacgt ggcgatcgtg ccccaggaat ttttcacttc
      661 tttgccaatg aacataagat ctttcattcc ttgatcgata gcagcgtatc gctcctccgg
      721 gctcaagata gccgcatcca cggcgccgga tagaagcgaa ggcagccgat tgaccgggcc
      781 gggatcgaca aagacaacat ctttgttgcc gtccatgccg tatttgccca tgatatttct
      841 gaacatgaac gctgcgatgg aggcaactcc cgtggtcccg acctttttac ctttaagatc
      901 cttaatcgat gaaatgttgg gccgcgctac cacccattgc agaacctgat cgttggcggc
      961 gataacagtt ttaaaaggcg cgccgccctt ggaaattgcc accagcgcgc tgctgcctga
     1021 accagaaaat tgcacgttgc ctgcagtcaa cgcctgtagg ccaggcgcat tctgcatata
     1081 aacaagctta agatcgatgc cctcttcctt caggtatcct cggtcctgag caatcggcaa
     1141 tatcgtgtac tgaaaagcct ttgacgttag tgcaacgttt acagtctcct gaccgtgtcc
     1201 gaccctggcc gaagtaccga agcaaatgac gatacaaaaa aacagaggga caaacttaac
     1261 tgacattgaa agcacctcct ttgattttgg ccctgtggtc gggcctatgt tgatgcagat
     1321 tcttgagagc gccgaatctt agtgttcgtg agccgtaccg tactaccgca gcgtcgcttc
     1381 agtcaattaa aatgctctca cctgttcagc tacgcattga cagaacagtt caggcttgga
     1441 tacgctgatt tgtagatgac agaaaagagg aggagcggga tatgaaagcc attcgcattc
     1501 acaacttcgg cccaaccgaa gatgtgctcc agttcgaaga agtgccggtg ccggagcccg
     1561 gctctggcga gctattgatc aaagtcgagg cagcttcgct gaaccgagcc gatcttggct
     1621 tgcgtagcgg taattaccgc attgctccgg aagatttgcc gattattccc ggccgtgagt
     1681 tcgccgggcg agtgacgaag ctgggggaca gcgtcaatga attccaagtc gggcaacgcg
     1741 ttgtcgccta caccggcact ggcggctatg ccgagtacgc caaagcgaaa acatcagagg
     1801 tacggccgtt gcccgatggc gtggacgccg ccgcggcagc agcgattcca actgtctttt
     1861 tgacggcctg gttcggtctg atggaagaag gcaagctcaa atcaggcgag tggctgctgg
     1921 tgcaagccgg cagcagtggt gtcggtgtag cggcgattca gatcggcaag cacgcgggcg
     1981 ctaaagtagc tacaaccacg agcggcgatg agaaagcgcg caggctgcgc cagcttggtg
     2041 ccgatgcggt gatcgattac acccgaattg attttgttgc ggaaggcatg cgcgcgacag
     2101 gcaatcgcgg cttcgatgtc gtgctcgaga tgatcggcgg ggaggtctac cagaaaagta
     2161 tcgaggtcct tgcgcccgcg ggtcggttga tttctatcgg cggggctttc ggccaaatcc
     2221 cggatacgcc tccagctctc gcggacggac gaaccgcgcg ccgcttctcg atcacgaact
     2281 acttaaaggc gcatcccgag gggtttaaac agctcgatgc tgccctgcaa tgggtgcgtg
     2341 atggaaaata ccaagtggtt atcgatcgaa gcttcccgtt ggccgaaaca cgctccgccc
     2401 aacgtcttct cgaagggcga gatcacttgg gtaagatcgt cttagttccc taagcgcgaa
     2461 caaagccgcg gcctctaaag gcgccaatca ggatcagtcg tcttagggct gttctccggc
     2521 tccggttcaa cattggcaac tgccggcgct ggttctttgc ttgcgcccat gagaagcaag
     2581 caaaattctt cagcatcttc gtaattaaca ggcgttccgc caatgctgcc ggtgccgcca
     2641 aggatctgaa tctcatttct cttgtgaaac cagaacagtg caatcagagc caggccggca
     2701 acgagcagtc catattgaat cagggacaag gtggctggcg agatagcaaa cagccgggtg
     2761 ttaaattcga tgccttccgg ctgattcatc tgattggcaa taatcagagc cagcgaagcg
     2821 agaaacaaaa tcaaaccgaa aagcagagcc cacctgcttc tttgccagct taaacgaact
     2881 gtggtgatcg cccggcgcgg gatgatcgct cggttttcgc cgaacatacc gtagtcttga
     2941 tgctggaaaa cgtggccggt ggtgagcact aactggcttt tatcggttag ccgcaaccag
     3001 ccaagttccc tctcgtcatt catcgttttc tccgtcatcc ggatataatg gcgttcagta
     3061 cgatttcagc ctcgtccgtc cttatgggcc gacgttgcgc aaagcacacc aaaacggcca
     3121 atcggctgcc ttaatttgct gccagtggat acattctttt ggggttctgc gtcatgatct
     3181 tctgcttagt ttccatcgcc aggtcttcac gagcgacgaa tttgctgaca ttttcgcgaa
     3241 attccgaacg tggttcatgg gggaaatcag tcgcgcagaa cagaatatcg tcacggagaa
     3301 gttcgacgat tcttggaatg ccgagttctt cgagttcgca atgaatgaat atgcggccgc
     3361 tcttaagatg ttcagaggga gcgaccttta gctcgggaac ctgcgcgctg cggtgctcgt
     3421 attccaggtc catgcgttcc atcaggtacg gcacccaacc gcaaccagcc tcggcgaatg
     3481 ccacttttag tctgggaaat ttgtcgaaaa caccgccgta gatcatcccg gtcatttgaa
     3541 tcatctgacc gaagccgtga ctcaaggtgc gcacctcgat caaacggtca aaggcatcaa
     3601 cgccgatgcc ttgagttggg gcggcatgaa ccgccagaag cgcgtccaat tcttgggccg
     3661 cctcatagac gggaaagtat ttctcgtcac cgaagggctg cctcattccg actgctggaa
     3721 gaatcgcgcc aaccatatta agttccttga ccgcccgccg taactctttg accgcctccg
     3781 gtatcgactg cagcggaatc agggccatgc cgcgaagccg cttttcctgc tttaaaaaca
     3841 gttcggaaag ataattgtta taaccttggg cgatcgcaca agcccagtcc ttgtcccgga
     3901 tcaagccaaa ggtgagcccg gcggtgggat aaagtaccga ccattcgatg tcatcttcat
     3961 ctagaaactc cttccattcc ttcagcgatc cctgcgccac atagcggcct ttgccgtcca
     4021 ccacgcgcaa agcttgacgg tgaaatccat ccaaggtcgg gaagaagggg aaggacaaca
     4081 gatcctccct tcccttgtaa ggctgcggca gaaaatctag cagggcacga tcattctcca
     4141 ccacatgtcc gtcagcatcg ataaatccct taaccttagt catcgaaact cctcacttcc
     4201 gtggtatgcc gctgggaaaa gttacgcaat acttttgggc gcggcttagc gtactagtta
     4261 tcaagaaaag ctcaggctct gcaacgcatg gcttccgttc atactgcctg cgccaagttg
     4321 atctgttgtc tccaggtgtt ttcggacaag gtctcggaag ttgccgcgag ctactcttta
     4381 gatcgatgtc atggatcatt taagatcatt ttgcgtgtca aaaactcacg aaccacatct
     4441 ttcgcgcgcc gctgttcggc atcgatggcg tagttgagct cacgcatctc ctcgacattg
     4501 agaatt
//
LOCUS       DASUTG010000158         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_469495, whole genome shotgun sequence.
ACCESSION   DASUTG010000158 DASUTG010000000
VERSION     DASUTG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469495"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..333)
                     /gene="rpsO"
                     /locus_tag="VF089_03580"
     CDS             complement(64..333)
                     /gene="rpsO"
                     /locus_tag="VF089_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6173070.1"
                     /translation="MGIAPQQKQGVIEQFRVHQSDTGSPEVQVALLSQRIDYLTQHFK
                     IHAKDHHSRRGLLKLVGQRRRLLDYLKKSDFERYQVLIQRLGIRK"
     gene            540..1151
                     /locus_tag="VF089_03585"
     CDS             540..1151
                     /locus_tag="VF089_03585"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF019889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX6173071.1"
                     /translation="MKISTAAALLQILLLILASLSQAADFFRWIDQHGVVHFTDNVHN
                     IPEARRSTAVRIKGQEPARPAAPSKISPATAAKASIPFEKRGQVVVVEVMLNKSVPVR
                     LVVDTGATYTMISAATAKELNIDAQRAQRTMPFQTANGVIQAPLANLESVIVGGMEIK
                     NLTAAIHDAVPSAQVAGLLGLNFLSNFRLDIDTEKGILHLEKK"
     gene            complement(1274..>3211)
                     /locus_tag="VF089_03590"
     CDS             complement(1274..>3211)
                     /locus_tag="VF089_03590"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6173072.1"
                     /translation="AYGSVFFQNANNSVRVTDDFMQAVQDDREWKTRFVKTGEVSDTY
                     RARDLMRMIAESTYFCGDPGMQYDTTINDWHTCSGTGRINASNPCSEYMHLDNSACNL
                     ASLNLLKFLRDDGVFDTESFRRTVDIIVLAQDILVDNSSYPTPEITANAHAFRELGLG
                     YANLGALLMSLGLPYDSDAGRGYASAVTALMSGEGYLQSSRIAAATGAFVGYDVNREP
                     MLRVLNKHRAHAYKISSAHVPLDLLTAARESWDQTCGEAEMHGVKNSQISVLAPTGTI
                     AFMMDCDTTGVEPDIALIKYKKLVGGGLLKIVNNTVARALKRLGYDVKQIQDIVEYIE
                     EHETIEGAPHLSDEDLAVFDCAFKPANGIRTINYMGHIRMMGAVQPFISGAISKTINM
                     PTEATVEDIIQAYMESWKLGLKAVAIYRDGSKRTQPLNTSKDKDETKNGTAENKETRP
                     LRRKLPDERRSITHKFDIAGHEGYIIAGMYEDGQPGEIFITMSKEGSTISGLMDSFAT
                     AISIALQYGVPLRGLVDKFSHMRFEPSGFTKNPDIPMAKSIMDYIFRWLATKFLDGDA
                     QREIGVVKQETEDKEVPVSKKAVSIVAQTPRGGAPVASISSVTSLYQQDAPPCPDCGA
                     IMIRSGACYKCLNCGAVSGCS"
BASE COUNT          735 a          867 c          843 g          766 t
ORIGIN      
        1 gctcctctga attgagaggt gctcgacgga gggtaaggat ggagggcaac cggttaggaa
       61 ccgttacttc cttattccaa gtcgttggat tagaacctga tatcgttcga aatcggattt
      121 cttcagataa tcaaggagcc ttctcctttg gcctaccagc ttcaataggc ctcgacggga
      181 atgatgatct ttagcatgaa tcttgaagtg ctgggtaaga tagtcgatgc gctgactcaa
      241 gagcgcgact tgcacttcag gagagccggt atcgctctga tggaccctga actgctcgat
      301 aaccccctgc ttttgctgtg gagcaattcc catgttctcc gctttttctc tctttctttt
      361 ttattatcga tgttttctaa catagtgact tactgttgta aagctgcacc tgggccaagc
      421 ggcttgagag gcgcggttgg tctcgcgttg acgtggctct agagcgcgat cggcccacag
      481 aatgcaaaaa caagagaact gaccgttttt ttatagcggc gttatgctaa aatcgagctg
      541 tgaaaatttc gaccgctgca gcgctattgc agattctgct cttgatttta gcctcgcttt
      601 cgcaggcagc ggattttttc cggtggattg accagcacgg cgttgttcat ttcaccgata
      661 acgtccacaa tattccggaa gcgcgccgat caacggcagt tcgaatcaaa gggcaagagc
      721 cggcgagacc cgcggcgcca tccaaaattt cgccagcaac agctgccaag gcatccattc
      781 cctttgaaaa acgcgggcag gtcgtcgtcg tcgaggtgat gctcaacaag agtgtccccg
      841 tcagattggt ggtcgacacc ggcgccacct acacgatgat ttcagcggca acagccaaag
      901 agttaaacat cgatgcacaa cgagctcagc gcacaatgcc ctttcaaacc gcgaacggtg
      961 tcatccaagc acccctcgcc aatttggaat ccgtgatcgt gggcggcatg gagataaaaa
     1021 atcttactgc agcgattcac gacgccgtgc caagcgccca ggtcgcaggt cttctcggcc
     1081 tcaacttcct gagcaacttc cgcttggaca tagataccga gaagggcata ctccatctgg
     1141 aaaagaaata agcagctctg acgatcccga gtaatcgcgt tggcgcactt tgatcgccta
     1201 ctgatgccgg gtgaaccaaa aggggacggc ggattggacc accgccccca tgttactgct
     1261 gagccagctg atcttaagag caaccgctga cggcaccgca gttgaggcac ttatagcaag
     1321 cgccgctgcg aatcatgata gcaccgcaat cgggacaggg cggcgcatct tgctggtaaa
     1381 ggctcgtgac actgctgata ctggccaccg gcgccccgcc ccgaggcgtc tgagcaacga
     1441 tcgatacggc tttcttagat accgggactt ccttatcttc ggtttcctgc ttgacgacgc
     1501 caatctcccg ctgggcatcg ccgtcgagaa acttggttgc cagccacctg aagatgtaat
     1561 ccatgatcga cttggccatg gggatatcgg gatttttagt aaacccagag ggttcgaagc
     1621 gcatatggct gaacttgtcg actaagcctc ttagcgggac gccgtattga agcgctatcg
     1681 aaatggccgt ggcgaaggaa tccatcagcc cggaaatagt tgagccttct ttggacatgg
     1741 tgatgaaaat ttccccgggc tggccgtctt cgtacatgcc ggcgatgata taaccttcgt
     1801 gaccggcgat atcgaacttg tgtgtaatcg agcgtcgttc gtctggcagc ttgcgccgca
     1861 gcgggcgcgt ttccttgttt tcagccgtgc catttttagt ctcgtctttg tcctttgatg
     1921 tattgagcgg ctgggtgcgt ttcgaaccat cccggtaaat cgccaccgcc tttaatccga
     1981 gcttccagga ctccatgtac gcttggataa tgtcttccac ggtcgcctca gtcggcatat
     2041 tgatggtttt agaaatcgca ccggagataa acggctgcac ggctcccatc attctgatgt
     2101 gccccatgta attaatcgta cggatgccgt tggccggctt gaaggcacag tcgaagacag
     2161 ccagatcctc gtcgctgagg tgcggcgcgc cttcgatggt ctcatgctct tcaatgtact
     2221 cgacgatgtc ttgaatttgc tttacgtcat accccaagcg cttaagcgcc cgagccaccg
     2281 tgttattgac gatcttcaag agtccgccgc ctaccagctt cttgtatttg atcagtgcga
     2341 tatcgggctc cactccggtc gtgtcgcaat ccatcatgaa agcaatcgtg ccggtcggcg
     2401 cgagcaccga gatctgcgaa ttttttacgc cgtgcatttc ggcctcgccg caggtctgat
     2461 cccacgactc ccgcgccgcc gtcaggagat ccaagggcac gtgcgccgac gaaatcttgt
     2521 atgcatgagc gcgatgtttg ttgagcactc gcagcatcgg ctcccgattg acatcatacc
     2581 ctacgaaagc tcctgtggcc gcagcgatgc gcgacgattg aagataccct tctcccgaca
     2641 tcagtgcggt caccgcagat gcatagccgc gaccggcatc ggaatcatag ggcaacccta
     2701 aggacatcag caatgcacca agattagcgt agcccaaacc caactcgcga aaggcatgtg
     2761 cattggcggt aatctccggg gtcggataag aagaattgtc gacgagaatg tcttgcgcaa
     2821 gcacgataat gtcgaccgtg cggcgaaaag attcagtatc gaaaacaccg tcgtcacgca
     2881 agaatttaag caagttcaac gaagctaaat tgcaagccga attgtcgaga tgcatgtatt
     2941 cggagcatgg attggaggca ttgatcctgc cggtgcctga acaggtatgc caatcgttga
     3001 tggtcgtatc gtattgcatt cccggatcac cgcagaaata agtgctctcg gcaatcatgc
     3061 gcatcaaatc acgtgcccta taggtgtcgc tgacttcgcc ggttttgacg aagcgggtct
     3121 tccattcacg atcgtcttgc acggcctgca taaaatcatc agtgacacgg acagagttat
     3181 tggcgttttg aaagaatacg ctgccgtagg c
//
LOCUS       DASUTG010000159         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_25426, whole genome shotgun sequence.
ACCESSION   DASUTG010000159 DASUTG010000000
VERSION     DASUTG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25426"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VF089_03595"
     CDS             complement(<1..818)
                     /locus_tag="VF089_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6173073.1"
                     /translation="MLTQVNADPEHPNGCICVKGAAAPDIVYSPDRLHHPMVRTRPKG
                     DPDPGWIRISWDQALNLTALRLNDIKDNYGAEAVVFSRATTAGSASIDFDGWLQRLAN
                     AFGSANLLTSNHICTWNRRVGSKYTFGTAMPLPDFDQTQCMLIWGINPAATSPAQALR
                     INRARRRGAKLIVIDPRKTTMAAKADCWLRVGPGTDGELAMAMIHVLIEEKLFDEEFT
                     RRWTNGSWLVRCDNNQLLTERDLQISGKPNTYIVWDEHDHALAAVGRNTAALFG"
     gene            complement(1169..2161)
                     /locus_tag="VF089_03600"
     CDS             complement(1169..2161)
                     /locus_tag="VF089_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173074.1"
                     /translation="MRRMLLSVLVTTFVATMTHPASAQIYEQLIAGAKKEGRLILYAS
                     ATAPQLQMYFAAFNKKYPFIKTEFFRTGKQALLSKVLLEEQAKQRIADVIHTSVIEIN
                     IMKNRGALSKYVPLEAAAYPAQYKDSEGLWTSAYASGTLLGFNSHQVKRTEAPKNYED
                     LLNPRWKGAIAIDSNKIEWFAMLLKLKGQAFMEKLAAQQPTVRSGNTLVLQLLAAGEF
                     PISAGVYEYSVEDLKTRGAPVDWIGLEPVITYTVAVALPSQPPHPFAAKLFTEWLLSK
                     EGQEVVNQYGRVPIRDDVDSKYGKILKQHKLLMTDVDLGQKEAEVNATFRKLFQ"
     gene            2411..>3239
                     /locus_tag="VF089_03605"
     CDS             2411..>3239
                     /locus_tag="VF089_03605"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX6173075.1"
                     /translation="MATPEFVYQDPFPIQKDTTKYRLLTKEHVSVTQFEGKEILKIAP
                     EALTYLSNIAIRDVSFLLRTAHLEKVAAILKDPEASDNDRYVALAMLRNADTAAKGIL
                     PFCQDTGTATIVGKKGQQVWTGVKDEEYLAKGIYKTYTEENLRYSQTAPLNMYDEVDT
                     GTNLPAQIDLMATDGMEYQFLFVTKGGGSANKTSLYQETKALLNPGSLEKFLTEKMIT
                     LGTAGCPPYHLAFVIGGTSAEACLKTVKLATTGYLDHLPTTGNVHGRAFRDVETEEKL
                     "
BASE COUNT          718 a          866 c          902 g          753 t
ORIGIN      
        1 ctgccaaaca gcgccgcagt attccgtccc actgcggcca gtgcgtgatc gtgttcgtcc
       61 cagacgatgt atgtgtttgg ctttccactg atctgcagat cacgttcggt gagaagttga
      121 ttattgtcgc agcggactag ccatgatccg ttcgtccaac gccgggtaaa ctcctcgtcg
      181 aaaagctttt cttcgataag aacgtggatc attgccatgg ccagctcgcc gtcagttccg
      241 ggaccaaccc gcaaccagca atcggctttt gccgccatgg tggttttccg cggatcgatg
      301 acgatcagtt tcgcgccacg ccgccgcgcc cggttgatgc gtaaggcttg cgccggtgat
      361 gtggcagccg gattgatgcc ccagatcagc atacattgag tctggtcgaa gtccggcagc
      421 ggcatggctg tgccgaaagt gtacttcgat ccgacccggc gattccatgt gcagatatga
      481 ttcgaggtga gcaggttggc actgccaaac gcgttggcca gacgctgcag ccagccatcg
      541 aagtcgatcg acgcgctccc ggccgttgtc gcccgggaga agaccaccgc ttcggcgcca
      601 tagttatcct ttatgtcatt aaggcgaagc gcggtgagat tgagcgcttg gtcccaactg
      661 atgcgaatcc aaccgggatc gggatcgcct ttgggcctgg ttcgtaccat tgggtgatgt
      721 agccggtcgg gcgagtaaac gatgtccggc gcggccgcgc ccttaacgca gatacaaccg
      781 ttggggtgct cagggtcggc attgacttgc gttagcaccc cgtcttcgac ggttgcgaca
      841 acgccgcaac gcgaagtgca catcgagcag taggtgtgta aattttgagt gacgataaat
      901 aaattcttta tcgaatcggc gaaagtttat cgaatcagag ttagcgctaa tccgacattt
      961 ttcgaatccg cgaggccagc gagccttaca gccgtgggca atgggccagt gccttaaagg
     1021 ccaccccaca ttcccctaag ggtgatgttt gtacctgggg ccttagagcc aactgcgtgc
     1081 tcgactgccc acggctttca ggctcgccat cctcgctcac tgctgacaac gctcaactat
     1141 cgtcggatta ggattattca atcggttctc actggaatag ttttcggaac gtggcgttaa
     1201 cttcggcttc cttttggcct agatcgacgt cggtcatgag caatttgtgt tgtttgagaa
     1261 tcttgccgta cttggaatcg acgtcgtcgc gaatcggcac ccggccgtac tggttgacga
     1321 cctcctggcc ttcttttgac aacagccact cggtaaaaag cttggctgca aacggatgag
     1381 gcggttgcga tggaagggcc acagcaaccg tgtaagtgat gaccggttcc aagccgatcc
     1441 agtccaccgg cgctcctctg gtcttcaggt cctctaccga gtattcgtac acgcccgcag
     1501 aaatcggaaa ttcacccgct gccaatagtt gcaagactaa ggtgttgccg gatcggacgg
     1561 taggttgctg ggcggcaagt ttctccatga aggcttgacc cttgagcttt agcagcatcg
     1621 cgaaccattc gatcttgttg gagtcgatgg cgatggcgcc tttccagcgc ggattcagta
     1681 gatcttcgta attcttgggc gcctcggtcc gtttaacttg gtgagagtta aaaccgagca
     1741 gggtgccgct ggcgtaggca gacgtccaaa gcccttctga atccttgtac tgcgccgggt
     1801 aagcggcggc ctcgagcgga acatatttgc tcagcgcgcc gcggtttttc atgatgttta
     1861 tttcgatgac gctggtgtgg atgacgtcgg cgattcgttg tttggcttgc tcttcgagga
     1921 ggactttact caacagcgct tgtttgccgg tgcgaaagaa ttccgtcttg ataaacggat
     1981 actttttatt aaacgcggcg aaatacatct gcagttgggg tgcggtggca gaggcgtaga
     2041 ggatcagcct accctccttt ttagcgccgg cgattagctg ctcatagatt tgcgccgagg
     2101 caggatgggt cattgtggca acaaaggtgg taacaagaac cgataggagc attcttctca
     2161 tgtgccctcc gtctgacgag aaactaatcg agcaggcgct gatctgtcaa cggtgcagac
     2221 tgggcaagcg tgaatgccgg ccgtgtcatt tcgatggagg gaaaatttgt gggtcatcca
     2281 gccacagtct cagccgaatt ctcgccgacg aatttggcgc acggcgtgcc aagcagacca
     2341 aatcacgaga ttgccgcacg tcgacaagcc ggtatttttt ggctaatatt ttgtttgagg
     2401 tttaaaaact atggcgactc ctgaattcgt ttatcaagat ccttttccga tacagaaaga
     2461 cacgaccaag taccgcctgc taaccaagga acatgtttcg gtgacgcagt ttgaaggcaa
     2521 agaaatatta aagattgcgc ccgaggcact gacgtatctc tcgaacattg ccatccggga
     2581 tgtgtctttc ttattgcgaa ccgcccatct tgaaaaggtt gcggcgatcc tcaaggaccc
     2641 agaggcctcc gacaacgatc gctatgtagc gctggccatg ctgcgcaatg ccgacaccgc
     2701 ggcaaagggc attctgccgt tttgccagga taccggcacg gccacgatcg tcggtaaaaa
     2761 aggccagcag gtttggaccg gagtcaagga cgaagaatac ctcgcgaaag ggatctataa
     2821 aacatatacc gaagaaaatc tgcgttactc gcaaactgcg ccattgaaca tgtacgacga
     2881 ggtcgatacc ggcaccaatt tgcctgcgca aatcgatctt atggccaccg acggcatgga
     2941 gtaccagttc ctgttcgtca ccaagggcgg aggatcggcc aacaagacct cgctgtacca
     3001 ggaaaccaaa gcactactga atcccggcag tttggaaaag tttctcacgg aaaaaatgat
     3061 taccctcggc accgccggtt gcccgccgta tcacctggcg ttcgtcatcg gcggaacctc
     3121 ggccgaagct tgtctcaaga cggtcaaact tgcgaccacc ggttacctcg atcatctgcc
     3181 gaccacgggc aatgttcacg ggcgcgcatt ccgcgatgtc gagaccgagg agaagctgt
//
LOCUS       DASUTG010000160         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_766243, whole genome shotgun sequence.
ACCESSION   DASUTG010000160 DASUTG010000000
VERSION     DASUTG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766243"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1134
                     /locus_tag="VF089_03610"
     CDS             109..1134
                     /locus_tag="VF089_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6173076.1"
                     /translation="MRNLRFFLGVLTIVLSIAASSGHLYSEQDGLHIIDVVRLDSRFD
                     KLVPSNLKIEKIAGGHKWVEGPVWNRQEGYLLFSDIPNNSIYRWQEDRGESLFLKPSG
                     YTGKAPFGGAEPGSNGLAFDPAGRLALAQHGDRRIVRFERSGRKTTLVDRYEGKRINS
                     PNDLVFKSNGDLYFTDPPFGLTKSFDDPHKELPFQGVYKFSKDGKLTLLTKDIKAPNG
                     IAFSPDEKKLYVSNADASNAIWMVYDVKPDGTIATGKTLFNATAWTKNKLGVPDGMKI
                     DKDGNLFAAGPGGIHVIAPDGTHLGSIETGAPTGNLAWGEDGSTLFITSNKTVYRLKL
                     GTKGVGF"
     gene            1270..2007
                     /locus_tag="VF089_03615"
     CDS             1270..2007
                     /locus_tag="VF089_03615"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HEX6173077.1"
                     /translation="MVTKLIGISAGLMLSVAGVSAADDGMATKFTVRIENTTKPDAFT
                     ASNGEKWSLAFSPGMAVVHTEKAPIFSTGKKDRSQGLEAQSEDGDPSMLAKSLQSVKG
                     IKSLVVFTTPIGAAGPGPITPGAAYEFTFSAIAGDRLSLTTMMGQSNDWFYAPAESGI
                     DLFENGRGISGDITSQMVLWDAGTEVDQEPGIGSDQGPRQKAPNTGKAENGVVRNAKD
                     VKYGSAYTKVSSVMRVTIMPVQAPGSN"
BASE COUNT          500 a          545 c          560 g          404 t
ORIGIN      
        1 tgcgcaagat ttcaggcccg cttcgtagag cggattcttc ctgctcggta agaacctagt
       61 tccggcgctt tcaggtgaga gttcaaaatc attctacgga ggatagcgat gcgcaatctt
      121 cgatttttcc ttggagtttt aacgattgtt ctttcgatcg cagcatcctc cgggcatctc
      181 tacagtgaac aagacggtct ccatatcatc gatgttgtgc gcctcgattc tcgtttcgac
      241 aagctcgtgc cgtcgaatct gaagatcgag aagatcgccg gcggccacaa atgggtggaa
      301 ggaccggtgt ggaaccgcca agaaggatac ctgctcttct cggacatccc gaacaattcg
      361 atctatagat ggcaggaaga tagaggcgaa agcctgttcc taaaacccag cggttatacc
      421 ggcaaagctc cattcggagg agccgagccg ggctcgaacg ggctggcgtt cgatcccgcg
      481 ggacggctgg cactggcgca acacggcgac cgccgcatcg tccgcttcga gagaagcgga
      541 cgcaagacca cgctggtgga tcgttacgag ggaaagcgta tcaacagccc gaatgatctg
      601 gtcttcaagt caaacggcga tctttatttt accgatccgc catttggctt aacgaaatcc
      661 ttcgacgatc cgcacaaaga actgccgttc cagggcgtct acaaattctc caaagacggc
      721 aaactgacgc tcctgacaaa ggacatcaaa gccccgaacg gcatcgcgtt ctcgccggac
      781 gagaaaaagc tctatgtcag caatgccgac gcgagtaatg ccatctggat ggtctatgac
      841 gtgaaaccgg atggcaccat cgcgactgga aaaactttgt ttaatgctac ggcctggacg
      901 aagaataaac ttggcgtgcc ggacggcatg aagatcgaca aagatggtaa cctgtttgcc
      961 gccgggcccg ggggtattca tgtcatcgcg cccgacggca ctcatctcgg cagcatagag
     1021 acaggcgcgc cgacaggcaa tctggcgtgg ggcgaggacg gatcaactct tttcatcacc
     1081 tcgaacaaaa ccgtttatcg cttgaagctc gggacaaaag gcgttggatt ctagccggca
     1141 tccctggcgc cgccagatct cgtgcagtcc agacgcatcc tttgccgcag gaccgccatc
     1201 taatccttca cgcacctttc gaatgattca gtgcgagttc gcaaaacaaa actcacaagg
     1261 agggaaatca tggttacgaa gttaataggt atatctgcgg gcttgatgtt gagtgtggcc
     1321 ggagtctcag cggcagatga cggcatggcg acaaaattta ccgtacggat cgagaacacc
     1381 accaagccgg atgcgttcac ggcatcgaac ggcgaaaagt ggtcgcttgc attctcgccc
     1441 ggaatggcag tggtgcatac tgagaaggcg ccaattttca gcaccgggaa aaaggaccgc
     1501 agccaaggac tcgaagcgca gtccgaagac ggcgacccaa gtatgctcgc caagtcgctt
     1561 cagagtgtca aaggaataaa atcgctcgta gtgtttacca cgcccattgg cgcggccggc
     1621 ccaggcccga tcactccagg cgcagcttac gaatttacgt tttctgcaat cgcgggcgat
     1681 cgactatcgc tgacgacgat gatgggccag tccaatgact ggttctacgc tccagccgaa
     1741 tcaggcatcg atctgttcga gaacggcagg ggcatcagcg gagatatcac atcgcaaatg
     1801 gttctctggg atgccggcac ggaagtcgat caggaacccg gcatcggctc cgaccaagga
     1861 ccgcggcaga aagcgcccaa taccggtaaa gccgagaatg gcgtcgtgcg caatgcgaag
     1921 gatgtgaaat acggcagcgc ttatacgaag gtgtctagcg ttatgcgtgt caccatcatg
     1981 cccgtgcaag cgccaggctc taattgact
//
LOCUS       DASUTG010000161         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_173598, whole genome shotgun sequence.
ACCESSION   DASUTG010000161 DASUTG010000000
VERSION     DASUTG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173598"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1240)
                     /locus_tag="VF089_03620"
     CDS             complement(143..1240)
                     /locus_tag="VF089_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX6173078.1"
                     /translation="MDRRSFLKSSLTVSAGLALGASPKLNRSASADDPTKWRSFEVIT
                     KVDIIKPVGATRVWLPVPLTKQTDYFTREDDSWTGNFKSARVVQYDRYGTGMLFAEWP
                     AGETAPSLELKSRFKTRDRQVNLSEKPNVAIKEDKAVLDYFRKPSKLIRTDGIVGTTS
                     RGITMGMKSDVDKARAIYDWIVDNTFRDPKVKGCGIGDISTMLETGYLGGKCADLNAL
                     YVGLARAAGLPARDVYGIRCANSSEFKSLGKADDITRAQHCRAEVFLSGYGWVPVDPA
                     DVRKVVLEENLPGQPLPLDDPKVKKARAKLFGAWEMNWLAYNYAHDVKLPNSNGPELG
                     FLMYPQVEEANGRRDSLDPDNFKYTIKAKEI"
     gene            complement(1362..1835)
                     /locus_tag="VF089_03625"
     CDS             complement(1362..1835)
                     /locus_tag="VF089_03625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1701..1703),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6173079.1"
                     /translation="MKSLEGQKAPAFSLEGHDGKTHSLEDYRGQKVILFFYPKDSTPG
                     UTKEACGFRELNTEMKSLGAVVLGISKDTITSHKKFAEKFNLPFVLLSDPKADVMKKY
                     QAFGKKMMYGKTVEGTIRSTVVIGPKGNIIKHWPTVKKADQHPHEVLAFLQEQAG"
     gene            complement(1899..2231)
                     /locus_tag="VF089_03630"
     CDS             complement(1899..2231)
                     /locus_tag="VF089_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173080.1"
                     /translation="MYLLNLATCMAESTESFCHGLAQYLEIRLGIPTQCVAGIPWQER
                     ERLLVPLGPITSPGPISGSTSCARTSLVSVTANGFSKRSTRFVAAHDTDYDPIRRMAD
                     VAASISLI"
     gene            complement(2284..>3353)
                     /locus_tag="VF089_03635"
     CDS             complement(2284..>3353)
                     /locus_tag="VF089_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6173081.1"
                     /translation="PEVPDNWPDLDAVLKYRDQVRSAVLEALDSMTRLESSDIMAQQG
                     RAFEMVLEHEYMHQETLLYMMHELPLDKKTRPAKSPRYSFHSAARCYPVEIPRGKVLL
                     GARFSELDFGWDNEFSQTTVDVPAFTMDSMPVTNGEYFEFVQSGAYDVERYWRPEDWR
                     WKVQTARKHPSVWLQRGSLWLYKAMFDLFPLAQVSSWPVYVTVAEARAYASWRGKRLP
                     SEAEFQRACYGGPDEISTRYPWGELAPAHRHGNFDLNSWSPNPIGSHPAGVSRWGIAE
                     LIGNGWELTDTPFAPFPGFTPYMPRYTDYSKDFFDGKHFVVKGASWATPVELLRPTFR
                     NWYQGHYPYVFAKFRCVSSKS"
BASE COUNT          775 a          902 c          862 g          814 t
ORIGIN      
        1 ccctcgactt cgctttattg gacgactcaa tcagtcgcaa attcaaggca gtccgacgga
       61 tcggaaaata gcagatgggt taagttaatc ccgaggcacg ctatggatgc cccgcataga
      121 atgatagtga gacggctgcc gatcaaatct ctttggcctt gatggtgtat ttgaaattat
      181 cggggtcgag actgtcgcgg cggccgttgg cttcctctac ctggggatac ataagaaagc
      241 cgagttcagg tccgttagag ttgggcaact tgacatcatg agcgtagtta taggccagcc
      301 agttcatttc ccaggcgcca aatagtttgg ctcgcgcctt cttgaccttg ggatcgtcaa
      361 gcgggagagg ctgaccgggt aggttctctt ccagaaccac cttgcgcacg tcggctgggt
      421 cgaccggtac ccagccgtaa ccggacaaga acacttcagc cctgcaatgt tgggcgcgag
      481 ttatgtcgtc tgccttgccg agacttttga attcggatga gttggcgcag cggattccgt
      541 aaacgtcccg tgccggcagt ccggcggcgc gggccagacc cacataaagc gcgtttaggt
      601 cagcgcactt gccgccgagg tagccggttt caagcatggt cgagatatcg ccgataccac
      661 agcctttcac cttcggatcg cgaaaagtgt tatcgacgat ccagtcatag atcgcgcgcg
      721 ctttgtcgac gtcgctcttc atacccatgg taattccacg tgaagtggta ccgacgatac
      781 catcggttcg tataagcttt gacggcttac ggaaatagtc cagcacggcc ttatcttctt
      841 tgatcgcaac atttggtttc tcgctcaaat tcacttgccg gtcgcgggtc ttaaaacggc
      901 tcttcaactc taatgaaggc gcggtttcac cggccggcca ttcggcaaat aacattcctg
      961 ttccatatcg atcgtactga acaactcgag cagacttgaa gttcccagtc cagctatcat
     1021 cctcacgtgt gaaatagtcc gtctgcttgg taagtgggac tgggagccat acgcgagttg
     1081 cccctaccgg tttgatgata tcgactttgg taatcacctc gaagcttcgc cacttcgtcg
     1141 gatcgtcagc actcgcagaa cgattcaact tgggcgacgc gcccagcgcc agtcccgcag
     1201 aaaccgtcaa gctcgatttt agaaatgatc tccggtccat gaactccccc tattggtttt
     1261 atgaatgcat ttgattggta atctctacaa ccgtttacca agaaaaacaa cgcaagaaca
     1321 accgataggt tctaagactt tcggaccata tcgatagttc ttcaccctgc ttgctcctgt
     1381 agaaaagcca gcacctcgtg cgggtgctga tcagcttttt tcacggttgg ccagtgtttg
     1441 atgatgttgc cttttgggcc aattaccact gttgatcgga tcgtaccctc cacggttttt
     1501 ccgtacatca tcttcttgcc gaaggcctgg tactttttca tgacgtctgc tttaggatcg
     1561 gacaacagca cgaacggcag attgaacttc tcggcaaact ttttatgaga agtaatggtg
     1621 tctttgctga tacccagaac taccgctccc agacttttca tttctgtatt tagctcgcgg
     1681 aacccgcaag cttccttggt tcagcccgga gtactgtctt ttggataaaa gaataaaatg
     1741 accttttgac cgcgataatc ctcaagcgag tgcgtcttgc cgtcatggcc ttccagactg
     1801 aacgccggcg ctttctgccc ctccaaactc ttcatgccga gcctttctcc ctctagtgag
     1861 aatgtacatt tattgcgcga attgatcgtt tctaggaatc aaatcaggga tatgcttgcg
     1921 gcgacatcgg ccatgcgacg aatgggatcg tagtcggtat cgtgtgccgc gacaaaacgc
     1981 gtcgacctct tggaaaaacc gttggccgta accgaaacca gcgaagtacg cgcgcacgac
     2041 gttgaaccgg aaatgggacc tgggctcgtt ataggcccaa gaggaaccaa aagccgctca
     2101 cgctcctgcc aggggatccc tgccacacat tgggtgggga tcccaagtct tatttccagg
     2161 tattgcgcca gaccatgaca gaagctctcg gtgctttccg ccatgcaggt ggcgagattt
     2221 agcaagtaca tctgtcagcg aatgcagggc tccaacattt catgagacga atcatataaa
     2281 ttcttagctt ttgctcgaaa cgcagcgaaa tttggcgaac acatatggat agtggccttg
     2341 ataccaattg cggaacgtcg gtcgcagcag ttccaccgga gttgcccagg aagcgccctt
     2401 aacaacaaag tgctttccat cgaaaaaatc tttggagtag tcagtatagc gaggcatata
     2461 gggcgtgaag cctggaaatg gcgcgaatgg cgtatcggtt aactcccagc cattcccgat
     2521 taattctgcg ataccccatc ggctcacgcc ggctggatgc gagcctatgg ggttcggcga
     2581 ccacgagtta agatcgaaat tgccatgacg atgcgccgga gctaattcgc cccatggata
     2641 acgcgtcgat atttcgtcag gtccgccgta gcaggcgcgt tgaaactccg cctcgctagg
     2701 caatcgcttt ccgcgccagc tagcgtaggc acgcgcctca gcaacggtca cgtagaccgg
     2761 ccaactggaa acctgggcga gaggaaagag atcgaacatc gctttgtaga gccagaggct
     2821 gccccgttga agccatacac tggggtgttt cctggcggtt tgcactttcc agcgccaatc
     2881 ttccggtcgc cagtaacgct ctacatcgta agcgccggac tgcacgaatt cgaaatactc
     2941 gccattggtg acgggcatag aatccattgt aaatgccggc acatcgacag tcgtttgcga
     3001 gaactcgttg tcccagccaa agtctaactc ggagaacctt gctccgagta aaaccttccc
     3061 gcgtgggatt tccacgggat aacaacgcgc ggctgagtgg aaggaatagc gtggcgattt
     3121 ggctgggcgg gtttttttat caagcggcaa ctcatgcatc atatacaaca gagtttcttg
     3181 atgcatgtat tcatgctcga gaaccatttc gaaagctcgc ccctgttgcg ccatgatgtc
     3241 cgaggactcc aaacgcgtca tgctatcgag cgcttccagg acggccgagc ggacctggtc
     3301 ccggtatttt aatacggcat ccagatcggg ccagttatcc ggaacctcgg gat
//
LOCUS       DASUTG010000162         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2101441, whole genome shotgun sequence.
ACCESSION   DASUTG010000162 DASUTG010000000
VERSION     DASUTG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101441"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1205)
                     /locus_tag="VF089_03640"
     CDS             complement(144..1205)
                     /locus_tag="VF089_03640"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173082.1"
                     /translation="MNRSSKFRGLHLRLERLRDYFKSHSKVKASSLRCVVLAKLILML
                     VLISSQPASSADAVKEKVAFAYAAISPSMSGIWMAKEIGAFDRNGLHAELVHISSGAI
                     AIQALVGGSVHAALGASNAVVAAIIKGAPIIAVGSNTSRPGMQLWVQPDIERAEQLQG
                     KTLAITRFGSTSDFVTRLIMRKLNLEGKAELRQFGGVVEADVGFRAGQAEGRVSSQAP
                     GPKAKMLVDAAELGIPFSMNLLAVSNEFYRRSPKTVERIVASYIEGIAALKTRKAQGL
                     KVLEKYMGQRGGSAEQHYEFVTKYLDVIPRVDPAAIETVLEMVGQKSPPKMQLFDNAI
                     VDRLAQEGFIDRLYKGGKS"
     gene            complement(1237..1947)
                     /locus_tag="VF089_03645"
     CDS             complement(1237..1947)
                     /locus_tag="VF089_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6173083.1"
                     /translation="MDANGWNTAFGELARATMSSPELERYYSVPITKPRAQLILKQLS
                     LYVRHRRDCWAYVSGNCPVLAVKQKILAHEYEEMIRDAHSEHGHLDLIIRQGKPIGLT
                     AEDILQTKPIPTTAATLYAWGWMCKKKSWLEGLAAMTMTEWNQDDRLLADLGGGHSAR
                     MGNLWVKYLGITWDDLPNWKAHREADKGHVDMFLPVWEEFGSGENREMILQAAKESEE
                     LYRLYQKGLADAMQALPD"
     gene            complement(2025..2732)
                     /locus_tag="VF089_03650"
     CDS             complement(2025..2732)
                     /locus_tag="VF089_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6173084.1"
                     /translation="MKRAEWRRELGELVRDAMRAPELDRYFEVKITRPGAQIFITQLG
                     LFIRHRRDCWAHVSGNCPQMSVKQKILQHEYGEVIKDEYTEHGHLDLIVRQGKALGLS
                     PEEVLEVEPLPTTFATLYAWAWMTREKSWIEGLAALTITEWANDDRLLQDLGGGLSSR
                     MAKRWMDDLGFGWEQMPNFKVHSEADEEHSDMFLEDLEKFAIGEKEALAYRAAKESME
                     LLKIFRAGIAQEMAKFA"
     gene            complement(2745..>3008)
                     /locus_tag="VF089_03655"
     CDS             complement(2745..>3008)
                     /locus_tag="VF089_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173085.1"
                     /translation="EGVAGLRSRKDLAYKVLAKYLLGGGDSLDEAYDYAVKYLDRNAR
                     TDPAVIQTVLNWEGKKDTSVNEFFDNSIIDSIIKEGFVDRLYK"
BASE COUNT          639 a          897 c          741 g          731 t
ORIGIN      
        1 gtgccgcaca taaagacttt gctgcatcac cattacggcg gcgcgcggtt tggtcatgtt
       61 gatggaaaaa tagtggtcga gctccggtga tctgatgtag tcgcgcacca tttcacccat
      121 tctctcgtgc cactgcgatt cgttcacgac ttgcctcctt tatagagtcg gtcgataaaa
      181 ccttcctgcg ccaaacgatc gacgatggcg ttatcgaaga gctgcatctt gggcgggctc
      241 ttctggccga ccatctccaa tacagtttcg atggctgccg gatcgacccg cgggatcaca
      301 tccagatact ttgtaacgaa ctcgtagtgc tgttccgccg agccgccgcg ctgccccatg
      361 tatttctcga ggaccttgag accctgagct tttcgggttt tgagcgccgc gatcccctct
      421 atgtaggagg cgacgatcct ctccacggtc ttgggtgatc gccgatagaa ttcgttactg
      481 acggccagca ggttcattga gaacgggatg cccagctccg cggcgtccac caacatcttg
      541 gctttcgggc ccggcgcctg cgatgacacc ctgccctctg cttgcccggc gcgaaagccg
      601 acatccgctt cgaccacgcc gccgaactgg cgcaactcgg ccttaccttc cagattgagt
      661 ttgcgcatga tcaaccgggt gacaaagtcg ctggtagagc cgaatcgagt gatcgctaac
      721 gtcttaccct gaagctgctc cgcgcgctca atatcaggct gaacccagag ctgcatacca
      781 gggcggctag tattgctgcc caccgcgata atcggcgctc ctttgatgat cgcggcaacc
      841 actgcgttac tcgcacccaa cgcagcatga acactgccgc ccaccaatgc ctggattgcg
      901 atcgcacctg agctgatgtg aaccagctcg gcatgcaagc cgttgcgatc gaaggcaccg
      961 atttctttgg ccatccagat tccggacatg ctcgggctga tggcagcgta ggcaaaagca
     1021 actttctctt tgacggcgtc cgctgaagat gcaggttggg atgaaatcag caccaacatc
     1081 agaatgagtt tggcaagcac aacacaacgc aggctcgaag cttttacttt actatgggat
     1141 ttaaaatagt ctcggagtcg ctccaaccgg agatgtaaac cgcgaaactt acttgaccta
     1201 ttcattgtct gcctaaagca ctttgtcatt ggctcatcag tcagggagcg cctgcatcgc
     1261 gtcggcaaga cccttttgat aaaggcgata caactcttcg gattcttttg cggcctgcaa
     1321 tatcatttcg cggttctcgc cagagccgaa ctcctcccaa actggaagaa acatatcgac
     1381 gtgacctttg tcggcttcgc ggtgggcctt ccagttcggc agatcgtccc aggtgatgcc
     1441 cagatacttg acccagagat ttcccatgcg cgccgaatga ccgccgccaa gatcggccaa
     1501 aaggcgatcg tcttggttcc attcggtcat cgtcatcgcc gccaaacctt cgagccagct
     1561 ttttttcttg cacatccaac cccaggcgta caatgtcgcg gctgtagtcg ggatcggctt
     1621 agtttgcaaa atatcctcgg ccgtaagtcc gatcggtttt ccctggcgga tgatcaaatc
     1681 caaatgacca tgctcggagt gcgcatcacg gatcatttcc tcgtactcat gcgcgagtat
     1741 tttctgcttg actgccagca ccgggcagtt gcctgaaaca tacgcccagc agtcgcgccg
     1801 atgacgaacg tatagagaaa gctgctttag gatcagctgt gccctgggct tggttattgg
     1861 gaccgaatag tatcgctcca actccggcga agacatcgtc gcgcgggcta actccccgaa
     1921 agcggtgttc caaccgttcg catccataat aattttcctc gatattcaaa caaccgctgc
     1981 gcttgggcta tgcagtgtgc gagcgagtct ctgaggctct ccccctaagc aaactttgcc
     2041 atctcctgcg caattcccgc gcggaatatc ttcagcaact ccatcgactc tttggccgct
     2101 cggtaggcaa gcgcttcctt ctcgccgata gcgaactttt ccagatcttc caaaaacatg
     2161 tcgctatgct cttcgtcggc ctcgctgtga actttgaaat tcggcatttg ctcccagccg
     2221 aaaccaagat cgtccatcca gcgcttggcc atccggctgg agagtccgcc accgagatcc
     2281 tggagcaaac gatcatcatt ggcccactca gtaatggtca gagcggccag tccttcgatc
     2341 caactcttct ctctagtcat ccatgcccag gcatacagag tggcgaaagt tgtcggcaac
     2401 ggctcgacct ccaaaacttc ctcgggagag agtcctaagg cttttccctg ccggacgatc
     2461 aggtccagat gaccatgttc ggtgtattca tccttgatca cctcgccata ttcatgctgc
     2521 aggatctttt gcttaacgga catctgcggg cagttgccgg aaacgtgcgc ccaacaatcg
     2581 cgacggtgtc gaataaacag tcccaattgc gtgatgaaga tttgcgcgcc gggtcgggtg
     2641 atcttgacct caaaatagcg atccagttcc ggtgcgcgca tcgcgtcacg caccagctct
     2701 cccagctctc ggcgccactc agctcgcttc atgcggatct cccgttactt gtaaagcctg
     2761 tcgacgaaac cttctttgat gatcgaatcg atgatgctgt tgtcgaagaa ctcgtttacc
     2821 gacgtatctt tttttccttc ccaattcagt accgtttgaa tgaccgccgg gtctgttctc
     2881 gcgttacgat ccaaatattt gacggcatag tcataggctt cgtccagtga atcaccgcct
     2941 ccgaggagat atttggctag aactttatag gccaggtctt ttctgcttct aagccccgcc
     3001 actccttc
//
LOCUS       DASUTG010000163         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_173998, whole genome shotgun sequence.
ACCESSION   DASUTG010000163 DASUTG010000000
VERSION     DASUTG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173998"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VF089_03660"
     CDS             <1..340
                     /locus_tag="VF089_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173086.1"
                     /translation="RRDVVNSLYDHIITFRHNELKDAWAAIYRAEAALAKSKSANAAQ
                     GKTLVAEARKVASQVPINDQRASDKEVTGAFRSKSGLKAQLETEWENLAKANYAKARE
                     LAAKALASAK"
     gene            397..2103
                     /locus_tag="VF089_03665"
     CDS             397..2103
                     /locus_tag="VF089_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6173087.1"
                     /translation="MELTQVGVAARWRAGVDWWQNGILLVLLVILTAFILYPVLSVLW
                     ISLSNDQGGLTFIHFANFFHRPLFRESLWNTLLSGALVVFFGALLGVPLAYIIARYDF
                     RGKLLLQTAATLPLVVPPFVGAVALQLILGRSGMVNLLLMSWFDTTIPFMEGLTGVIL
                     VQTLHFFPFILLNTVVSLSNIDSSLEEAAQNLGCKGIRLFRRVTLPLMLPGFTAGALL
                     TFIRAIDDLGTPLMLNYKNLLAPQAYLRITTIGMDDVDGYVVCVVLVVISLASLLAAR
                     KYLSLAEYATVQRSASSAKPLYGKRLALVWLISALILAVSLLPHIGIVLLSFSKIWSF
                     TLLPSTYTLDNYADIFFRVPHFVVNTLIYTILAATLDVILGAVIAFLLLRSRVRGRNV
                     LDAIATMPLAIPGVVLAVGYLRVFHGWDFPGIGAPLTSSWLILVIAYTMRRLPYTVRA
                     SYAALQQVHVTLEESAQSLGANRFGTFRKITLPMITGGLLAGGLIAFVTSSVELASTI
                     MLVPRIELGPISYGIYLYMQSPLGRGAGAALGVISILLVAIGTYLTQRIFGGRAGNAF
                     RI"
     gene            2087..3199
                     /locus_tag="VF089_03670"
     CDS             2087..3199
                     /locus_tag="VF089_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017436144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173088.1"
                     /translation="MLFVSDMQAASVRLENVGKRYGDHWVVCSVTLDIRQGEFFTFLG
                     PSGCGKTTLLRIIAGFVAPDEGVVYLDEQPMNHVPPWRRDIGLVFQNYALWPHLSVFE
                     NVAFGLRERKVARGEIERRVAAALKQVDLAGAEKRRPSQLSGGQQQRVALARTLVIQP
                     RVLLLDEPLSNLDAKLRIEMRLELLKLQRDLGLTTIYVTHDQEEALAMSTRIAVMRDG
                     KVIQQGKPREIYEQPADKFVASFLGHANLFSGQVISSGEGSIDVDVGGGLVIRAGCMT
                     ASSHPRGGETLFLSVRPEAVELQEIGTVCNGANHIEGHVIAAAYRGSLIEYEIAAGDH
                     VIKAHVVNPKGKPLFERNQPVCLRFAREDVTLVSGD"
BASE COUNT          649 a          927 c          953 g          805 t
ORIGIN      
        1 acggcgcgat gtggtgaatt ctctgtacga tcatatcatc accttccggc ataacgagtt
       61 gaaagacgcg tgggccgcta tctaccgggc tgaagcggcg ctcgccaaga gcaaaagcgc
      121 caatgccgct caagggaaaa ccctcgttgc cgaagccagg aaagtcgcga gccaggtgcc
      181 aatcaatgat cagcgagcta gtgacaagga agtgacgggt gcgtttagat cgaagtcagg
      241 cctcaaggcg caacttgaaa ccgaatggga aaacctggcc aaggcgaact acgccaaggc
      301 aagagagctg gcagctaaag cgctcgcttc agccaagtaa ccgaatttgg aattcgcgcg
      361 cggatgttcg gcagctgagc tggctacgag agtcctatgg agctgactca agtcggcgtt
      421 gctgcgcgct ggcgcgcagg cgtagactgg tggcaaaacg gcattcttct agtgctgctg
      481 gtcattctca cggcgtttat tctttatccg gtcttgagtg tcctgtggat ctcgctttcc
      541 aatgatcaag gcggcctgac ctttattcac ttcgctaatt tctttcatcg cccgcttttc
      601 cgcgagtcct tgtggaacac cttgctcagc ggcgctctcg tggttttttt tggcgcgctt
      661 ttgggagttc cgctggctta catcatcgcg cgttatgatt ttagggggaa attgttactt
      721 cagaccgcgg cgactcttcc tttggtcgtc ccgccatttg tcggcgccgt ggcgctgcaa
      781 cttatcttgg gccgcagcgg catggtcaat ctgcttttga tgagctggtt cgatacgacg
      841 attccgttca tggaagggct gaccggcgtc attctcgtgc aaaccttgca cttcttccca
      901 ttcattttac tcaacaccgt tgtgtcgctg tcgaacatcg actcctctct cgaagaggcg
      961 gcgcaaaacc tcggctgtaa aggcattcgc cttttccggc gcgtgaccct gccgttgatg
     1021 cttcccggct ttaccgccgg cgctctcctg acgtttattc gagcgatcga cgatctgggc
     1081 acgccgttga tgctgaacta caagaacctg ctcgcacccc aggcgtacct gagaatcacg
     1141 accatcggca tggacgacgt cgacggctat gtcgtctgcg tcgtgctggt ggtgatttct
     1201 ttggcttcgc tgttggcggc ccgcaagtat ctaagccttg ccgaatacgc caccgtgcag
     1261 cgctccgctt cgagcgccaa gccgctctac ggcaaaaggc tggcgctcgt gtggctgatc
     1321 agcgcgctta ttttggccgt gagcctgttg cctcacatcg gcatcgtttt gctgtcgttc
     1381 tccaagatct ggagctttac gctgctgccg tccacgtata cgttggacaa ctatgcggat
     1441 attttttttc gggtgcctca cttcgtggtc aacacgttga tctatactat cctcgcggca
     1501 acgctcgatg tcattcttgg cgccgtgatc gcattcctgc tgttgcgcag ccgggtgcgg
     1561 ggaaggaatg ttcttgacgc catcgcgacg atgccgctgg cgattccagg cgtggttctc
     1621 gctgtcggct atctgcgcgt ttttcatggt tgggatttcc ccggaattgg cgcgccgttg
     1681 acctcatcct ggctgattct ggtgatcgct tacacgatgc gacggctgcc ctacaccgtg
     1741 cgcgccagct acgccgctct ccaacaagtt catgtcactc tcgaagaatc ggcgcaaagt
     1801 ctcggagcga accggtttgg cactttcagg aaaattaccc tgccgatgat caccggaggc
     1861 ttgctggccg gcggattgat cgctttcgtc acttcatccg tggagctcgc atccaccatc
     1921 atgctcgtgc cgcggattga attgggaccg atttcctatg gaatttacct ttacatgcag
     1981 agcccgctcg gccggggcgc gggcgctgcg ctcggggtga tatccattct gttggtcgcc
     2041 atcggaactt atctgacgca gcgaatcttc ggcggccgcg ccggtaatgc ttttcgtatc
     2101 tgacatgcaa gcggctagtg tcagactgga aaatgtcgga aaacgttacg gcgaccactg
     2161 ggtagtttgc agtgtcaccc tggatatcag gcagggagaa ttcttcacct ttctcggacc
     2221 cagcggctgc ggcaaaacca ccttgttgcg gatcattgcg ggatttgtcg caccggacga
     2281 gggggtggtt tatttggacg aacaaccgat gaaccacgtg ccgccctggc gccgggacat
     2341 cggcctggtt tttcaaaact atgccttgtg gccgcacctg agcgtcttcg agaacgtcgc
     2401 cttcggtttg cgagaacgaa aagttgcgcg cggcgagatc gagcgccgcg ttgctgcggc
     2461 tctcaagcaa gttgatcttg caggagctga gaaacgacgt ccatcacagc tttccggcgg
     2521 ccaacagcaa agagtcgctc tggcgcgcac actggtgatc cagccgcgag tgctgcttct
     2581 tgacgaacct ctcagcaatc tcgacgcgaa acttcgcata gaaatgcgcc tggagctgct
     2641 caagctgcaa cgagacctcg ggctgacgac gatttatgtg acgcatgatc aggaagaagc
     2701 tttagccatg tcgacccgca tcgccgttat gagggacggc aaggtcatac aacaaggtaa
     2761 gccgcgcgag atctacgagc aacccgccga caaatttgtc gcgtcatttt tggggcatgc
     2821 caatctattt tcaggtcaag tcatctcctc cggcgagggg tcgatcgacg ttgatgtcgg
     2881 cggcggcctc gtcatacgcg ccggctgcat gacggcgtcg agtcatcctc gaggtggaga
     2941 aacgcttttt ctcagtgttc gtcccgaggc cgtcgagctt caggagatcg gcacggtttg
     3001 caatggcgcc aatcacattg aaggacatgt gatcgcggct gcgtaccgag gatcgcttat
     3061 cgaatatgag attgctgccg gtgatcacgt catcaaagct cacgtcgtca atcccaaggg
     3121 aaaacccttg tttgaacgaa accagccagt gtgcctccgc ttcgctcgcg aggatgtaac
     3181 tttagtcagt ggtgattgac agcactcgat gatcattaca cgtctgcctg gatgtcgcgg
     3241 tcgagtggga aaggcggaga aaagaggtta tgtattctga tgcgccggct tgatgccgcc
     3301 cagggcccgg gacgtatttc caagcttagg ctgg
//
LOCUS       DASUTG010000164        12191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_470123, whole genome shotgun sequence.
ACCESSION   DASUTG010000164 DASUTG010000000
VERSION     DASUTG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470123"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /locus_tag="VF089_03675"
     CDS             complement(<1..1901)
                     /locus_tag="VF089_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173089.1"
                     /translation="MSGAVILNGAPITLTKILTDGLLYLYAAAMLLSAALLFMVQPMV
                     ARMILPFLGGAPAVWNTCLLFFQLLLLLGYLYAHLAGKWLGIKRHMVVHLALIGAALY
                     FLPVTIPADLLERLDINPVHKVLWVLLLSVGFPFLVLSANAPLLQNCFSTTDHRAARD
                     PYFLYAASNAGSLLGLFAYPFWLEPRLSLGQQAWFWLYVYVGFFVIAILCGLTLHSSA
                     TTRVNSPAELDPAGDDAPSSTRINFLRRLRWIVLSFIPSSLLLGVTTYITTDLASVPL
                     LWVLPLSLYLISFIVAYQRGSWASHPFIVKRQAFLLLAAAITFFSMATSPAVILLPLH
                     LLAFFVTCLVCHGELAKERPAIRHLTEYYLWISFGGVLGGFFNGFLAPLIFTRVQEYP
                     LAIIAAALVRPRLEANAGNPASYARDFIWAIALFLIILSVVLWMDRSAVLSARQTHFM
                     IFGLAGLIGLSFARRPLRFGLGIGAIMLATSWYNGPYGEVLLSDRSFFGVYRAIRDPE
                     RNVHVIFHGTTLHGAQNLDPKKRLEPLSYYHPSGPAGEAFKALAENGLPKPVAIVGLG
                     AGAMACYGQRGQDFVFYEIDALVERLARDQALFTYLRDCPARVSVHIGDARVTVASAA
                     DQAYSVFVL"
     gene            2163..3269
                     /locus_tag="VF089_03680"
     CDS             2163..3269
                     /locus_tag="VF089_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6173090.1"
                     /translation="MMQSNPLRVGLIGAGGRWGPRAHVPALKGVPETELYAVCTAHAE
                     TARAAAGKFGVSRAYGNDKELSADANVEAVVVAVRVPAHYQLTKNAIEAGKHVFCEWP
                     LGANTKEAEELAALARQKNVRTMVGLQRRASPAYLYMRELIQQGYVGEVLAVNMTLMA
                     SGVLTRPSDRTWQRDVNLGANTLTITFAHVFDAMCMVVGELSELSAMISTQVPQWFET
                     DTKKYVDVTSPDNIAVHGRFENGALVNAYCGVHPYHGSGHRLEIYGREGTLAMIGGGE
                     GGGEQQRKIMGGRKDDKAMQELTVPEQLKWVPKAVRNDGPAYEVGQMWAKFAEAIRTG
                     SAIETDFDHAVRRHRTLDAIARASETGQRQKIAL"
     gene            3653..5098
                     /locus_tag="VF089_03685"
     CDS             3653..5098
                     /locus_tag="VF089_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173091.1"
                     /translation="MDRAAEIHDALKHPVIDGDGHWMEPIPIFLEYLSQVGGAGAVDQ
                     IRALWRRNQAWYRATWPERQHNRLRRTIWWGVTSNTLDKATALLPALLNERLPELGID
                     FAMIYPSFGLSINGIAQDDLHRAAVRAYNMMTADMFAPFATRFAPVAIIPSHTPEEAL
                     EELEYAVTQRGFKAIMLRGNEERPIPSAAQGIDRQKAAWYIDTIALDSPYDYEPLWRR
                     CVELGVAVTQHAGSGRWQDRASISNFTYNHVGHFAESNHAFARGVFLGGLVRRYPTLN
                     FGFMEGGVSWACQLCLDLIEHWEKRRREGLQYPSATSTAEMHRLIDRYGDQRLKANAA
                     EIMNNLDAFRPECSLEELSRPEYVFNDFEAAGIKSKEDVRAVFSDNFYFGCEADDRAT
                     MWAFDPRMGVRLRPVFSSDFTHFDVPDFREVIPEAFELVEKGFVTEEDFREFTFTNAA
                     RLHTRNNPDFFKGTVVEHAVGDELGLKVGCR"
     gene            5213..6499
                     /locus_tag="VF089_03690"
     CDS             5213..6499
                     /locus_tag="VF089_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6173092.1"
                     /translation="MHLSRSEERMRGLYCLRRSVLFVTTIFTLFGDANPAVGIPGADD
                     DGQKCYRATHPEAVIRHCSQTISSAKFPPKAMAMAFYRRANAYNQKSESDRAIRDYNE
                     AIRLNPGYADAFANRGAAYARKRDYDRAIQDYDEAIRLNPRHADAFSNRGVVYARKKD
                     YERAIQDYDEAIRLNPDHIGALYARGNAYRRRGDYDQAVRNYDQVVRLNPRHAQAYSN
                     RGLTYAAKGRDNLAIQDYDEAIRINPEHVNAYYNRGNAYRRTGAYGKAVENYTRVIRL
                     NPKHGNGYSSRGMVRFYQGEFASAAADFSEARRFAPDNLYLALLLHVAEARAGIQGQA
                     AQTEIARRGDLESWPGPILAMLVGKLPEHVILTAGTSSDQPEKRCQAYFYLGQRRLMQ
                     QKKSEAAEMFRQAAATQASTLFEFEAAGAELSRLRN"
     gene            6765..7094
                     /locus_tag="VF089_03695"
     CDS             6765..7094
                     /locus_tag="VF089_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173093.1"
                     /translation="MFIKAIALVAIILFAPAPSLAQNSGSVTSLETFNLADIADDSIV
                     VLSKSESGVPGVAHVKYVYVNSICSGGLQLVDYYGGEFDANDRRLRRDEGQRCSRESH
                     DTGGDED"
     gene            8131..9078
                     /locus_tag="VF089_03700"
     CDS             8131..9078
                     /locus_tag="VF089_03700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6173094.1"
                     /translation="MKFSDLSIDEIRHQCLDGQKPVSPQILNKLRRDPRQGVRKLLEI
                     LNRRYEQERAERLRLQNMLNFERVLWKSGIRTVAGVDEVGVGPLAGPVVAAAVVFPPN
                     TELDGVDDSKQLEPEQRTNLAARIREHATAIGVGSAEVGEIDRLNIYHAGLLAMRRAV
                     EALPCMPEHVLVDARVIPGVSVPQNSFNKGDGINFSIAAASIIAKTHRDRLMEDLEKE
                     YPGYGFAQHKGYSTPEHQNAIRELGPCPIHRLSFHFIRELCGEFSELFYTLKQQLQEA
                     NTAADLRGLERKLSERWSELDEREQRKIRLMLSRRWKTV"
     gene            complement(9810..10436)
                     /locus_tag="VF089_03705"
     CDS             complement(9810..10436)
                     /locus_tag="VF089_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6173095.1"
                     /translation="MMTLYFSPGACSLASHIGLEETGATYEIKPILLAKGQQRTEDYL
                     KINPRGKVPALNVDGKILVENTAILTYLARRFPEKRLMPADPAEEARCIGTMCWFSSV
                     VHPSYQRFHRPERFAESEAAHAGVKENGRKLFWANLQEIDSMIEANDWVMGREFTAVD
                     PYALVFYGWGERSGFPMKELSAYTAWQERMMKRPTVKKSVESEQNVSQ"
     gene            complement(10594..11616)
                     /locus_tag="VF089_03710"
     CDS             complement(10594..11616)
                     /locus_tag="VF089_03710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173096.1"
                     /translation="MRLSVLDQSTASKDRTQDTAIRETLELARRCDALGFHRYWVSEH
                     HNSGTIAGTAPEIVMAAIAATTSHIRIGSAGIMLPHYSALKVAEQFRVLEAIAPGRID
                     LGVGRAPGSDQMTSYALNPNPQNVLDQFPRQVQELRHWVSGIPLPEGHPFRTIVAQPT
                     GPSSPEIWILGSSDYGAQLAAYFGLPYAFAYFFSEGTGVEQALKLYRNNFQPSEKHPK
                     PMATICVWALAADSEEEALYQFKTRERAIIDRKQGIRLPLMPPDEADRPYSVAETRTA
                     EKLRRKAIVGSKDQVAARLDELAKHLELDELVVVTWTYDPAPRHRSYELLAQAFAMEE
                     AEASSA"
     gene            complement(11792..>12191)
                     /locus_tag="VF089_03715"
     CDS             complement(11792..>12191)
                     /locus_tag="VF089_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX6173097.1"
                     /translation="GEAVDLIKAGNPPRIVQDESKATYDPPCGDEHAKIDWSKPAQEV
                     YNLIRGCDPQPGAHTTYQGKTLRLFDARLQKNANGAEPGQVTGIGSEEIIIAVTGGTL
                     TVKRVRGEAGKISGTEFAKQVNLRVGDRLG"
BASE COUNT         2849 a         3274 c         3521 g         2547 t
ORIGIN      
        1 tcgagaacga agacgctgta cgcttgatcc gccgcactgg cgactgtcac ccgagcatcg
       61 ccgatgtgga ccgacacccg cgccgggcag tcgcgtagat aagtaaaaag agcctggtca
      121 cgcgcgaggc gctcaaccaa agcatcgatt tcgtaaaaga cgaaatcctg cccccgttgg
      181 ccatagcaag ccatggcgcc ggcaccgagt ccgacgatgg caacgggctt gggcagcccg
      241 ttttctgcca gggccttgaa tgcttcgcca gcgggtcctg agggatgata gtaagatagc
      301 ggctcaaggc gctttttcgg atcgaggttt tgcgctccgt gcaaggtggt gccgtggaaa
      361 atgacatgca cattgcgctc cggatcgcgt atcgcgcggt aaacaccgaa gaagcttctg
      421 tcgctcaaga gcacctcgcc atagggtccg ttgtaccaac tcgtcgccaa catgattgca
      481 ccgatgccca agccgaagcg cagcggccgt cgggcaaagc tcaagccgat caatccggca
      541 agcccaaaaa tcatgaaatg ggtttgacga gcggaaagaa ccgcgcttct gtccatccat
      601 aagaccacgc ttaagatgat gaggaagagc gcgatcgccc agataaaatc gcgtgcataa
      661 ctagcggggt ttccggcgtt tgcctccaac ctgggccgga ctaatgcggc ggcgatgata
      721 gcgagcggat attcttgcac ccgggtgaag attaacggcg caagaaaacc gttgaagaag
      781 ccgcccaaca caccgccgaa ggagatccac agataatatt ccgttaaatg ccggattgcg
      841 ggccgctcct tggccagctc accatggcac acgaggcagg tgacgaagaa ggcgagcagg
      901 tgcaagggca gcaatatcac ggcggggctg gttgccatcg aaaagaaagt gatcgccgcc
      961 gctaataaaa ggaaagcctg tctttttacg atgaagggat gcgacgccca tgaaccgcgt
     1021 tgataggcta cgatgaacga gatcagatac agtgaaagcg gcagcaccca taagaggggc
     1081 accgacgcga ggtcggtcgt tatgtaggtg gtcaccccca gcagcagact cgatggaatg
     1141 aacgaaagaa cgatccagcg caagcggcgg aggaaattaa ttctggtgct gcttggagca
     1201 tcgtccccag caggatcaag ctcggcgggt gaattcacgc gcgttgttgc cgagctatgc
     1261 agagtcaagc cgcaaaggat cgcgatgacg aaaaacccga cgtaaacgta aagccagaac
     1321 catgcctgct ggcccaggct cagccgcggc tctaaccaga acggataagc gaacagcccg
     1381 agcaggctgc cggcattgct tgctgcatag agaaaataag gatcgcgtgc tgcacggtga
     1441 tccgtcgtgg agaagcagtt ttgcaataag ggtgcgttcg cggaaagaac caggaacgga
     1501 aatcccacag agagcagcag tacccaaagc accttgtgga ccgggttgat atcgagccgc
     1561 tccagtaagt ctgccggaat tgttaccggc agaaagtaca gcgccgcgcc gatcagcgcc
     1621 aggtgcacga ccatgtgccg tttgatgccc agccacttac cggccaagtg cgcgtagaga
     1681 tagcctagca acagcagcaa ttgaaaaaag agcagacagg tattccacac cgccggcgcg
     1741 ccgcccagaa acggcaggat cattcgcgcc accatcggtt ggaccataaa cagcagcgcc
     1801 gcgctcagga gcattgcggc tgcgtagaga tagagcaggc cgtctgtgag aattttagtc
     1861 aacgttatgg gcgcgccgtt gagaattacg gcgccgctca ttttagacca actcggctgt
     1921 gacacgaaga tgtaatttcc ccggcctttc ttgcggcggg ttgaaataaa aacgcaatgt
     1981 gagacgtggt cagtccctgg ggcaatcacc aggttgttcg agttcgagcc caaactcgtt
     2041 gactaagact ttcattccgg actttcttgt cagatgggta taacgtaaga cccctgagct
     2101 gtgtctggtg taattgccgt tgtccttgcc aggcggcaaa ttgatttcac aggagggaaa
     2161 caatgatgca atccaatcca ctccgcgtag ggctcatcgg agcagggggc agatgggggc
     2221 cgcgtgcgca cgtgccggcg ctgaagggtg tgcctgaaac cgagctctac gccgtgtgca
     2281 ccgcgcacgc ggaaaccgcc cgggcggccg ccggtaagtt cggcgtgtcg cgcgcgtacg
     2341 gcaacgacaa agagctaagc gcggatgcga acgtcgaagc cgtggttgtc gcggtgcgcg
     2401 tcccggcgca ttatcagcta accaagaatg ccattgaagc aggcaagcac gtcttctgcg
     2461 aatggccgct cggggcaaac acgaaagagg ccgaagaact tgcggccttg gcgcgacaga
     2521 aaaacgtccg caccatggtc gggttgcagc gccgcgcctc gcccgcttat ctgtacatgc
     2581 gcgagctaat ccagcaaggt tatgtcggcg aagtgctggc cgtgaatatg acacttatgg
     2641 cgagcggtgt gttaacccgc ccttcggatc gcacctggca gcgcgatgtt aatttgggcg
     2701 cgaacacgct gacgatcacg ttcgctcacg tctttgacgc catgtgcatg gtggtcggcg
     2761 agctaagcga actctccgca atgatcagca cgcaggtgcc gcaatggttc gagaccgaca
     2821 caaaaaaata cgtggacgtc acttcgcccg acaacattgc ggttcacggc cgattcgaaa
     2881 acggcgccct cgtcaacgcc tactgcggcg tacatcccta tcacggcagt ggtcaccggc
     2941 tggagattta tggcagagag ggcaccttgg cgatgatcgg cggtggtgaa ggcggcgggg
     3001 agcagcagcg caagatcatg ggcggacgca aagatgacaa ggcgatgcag gagttgaccg
     3061 tgccggagca attgaagtgg gtgcctaaag cggtgcgcaa cgacggtccc gcctacgaag
     3121 tgggccagat gtgggccaaa tttgccgagg cgatacgcac tggctcggct atcgagaccg
     3181 acttcgatca tgcggtgcgc cgccaccgga cattggatgc gatcgcgcgc gcttcagaga
     3241 cggggcagcg gcagaagatt gccttataac aaagcctgtc ccagacgttc cgacaattcc
     3301 aatcgttaat aggcttcaga ggctccagtg ccttttgctc aggtccattg gattggctta
     3361 tgaaaacgat tggaactgct ggaacagagc ggagcggtgg aacggttgga atacttcgtt
     3421 ccaggcactt gcgattgacg gctcaccggc ttagctatag cctggtcgct tattgaaata
     3481 gggcgtggcc tgttgcaaaa agcagcttct tgcaggctgc tcaaaagatc cagacgcgag
     3541 gcgcgcaaaa aatcgacgag cggaagggta ctcagagtac gttgcggcga ggcgattgag
     3601 cgaaagaagc gtatgggtct ttcagtaggc tgcccgagag aggagcgtga ccatggatcg
     3661 agcggcagaa atacacgacg cgctcaagca tccagtgatc gacggcgacg gccattggat
     3721 ggagccgatt ccgatttttc tcgagtatct cagtcaagtg ggtggcgctg gggctgtcga
     3781 tcagataaga gcgctctggc gccgcaatca ggcgtggtat cgggccactt ggccggaacg
     3841 ccagcacaat cgtctgcgtc gcacgatatg gtggggtgta acgagcaaca cactcgacaa
     3901 ggcaacggcg ctcttgccag ccctgctgaa cgaacgcttg cccgagttgg gcatcgattt
     3961 cgcgatgatc tacccgagtt ttgggctaag catcaacggc atcgcccaag atgacctgca
     4021 cagggccgcg gttcgtgcct acaacatgat gacagctgac atgttcgcgc catttgccac
     4081 caggtttgcg ccggtagcta ttatcccgtc ccatacgccg gaggaagctc ttgaggagtt
     4141 ggaatatgcg gtgacgcagc gaggtttcaa agcgattatg ctgcgcggaa acgaggaacg
     4201 accgatcccc agcgcggcgc aaggaatcga ccggcagaag gccgcctggt acatcgatac
     4261 cattgctctg gacagcccct atgattatga gccgctctgg cggcgctgcg tcgagctcgg
     4321 cgtggccgtg actcaacacg ccggcagcgg tcggtggcag gatcgtgcct cgatcagcaa
     4381 cttcacttac aatcacgtcg gtcactttgc cgaatcaaac cacgccttcg cgcgcggggt
     4441 tttcctaggt ggtctcgtgc ggcgctaccc aacactcaac tttggcttca tggaaggggg
     4501 agtaagctgg gcctgtcagt tgtgtctcga cttgatcgaa cactgggaga agcgccgccg
     4561 cgaggggcta cagtatccaa gcgcaaccag caccgccgag atgcacaggc tgatcgaccg
     4621 atatggcgat caaagactga aagccaacgc cgccgagatc atgaacaact tggacgcatt
     4681 ccggcccgag tgcagcctgg aagagctgag ccgtcctgaa tatgttttca atgattttga
     4741 agccgcagga atcaaatcaa aagaggatgt tcgcgccgtc ttctccgaca acttctactt
     4801 cggttgcgag gcggacgacc gggcgacgat gtgggccttc gatccgcgca tgggtgtccg
     4861 gcttcggccg gtttttagct ccgacttcac gcatttcgac gtgcccgatt tcagggaggt
     4921 gattcctgaa gcgttcgagc tggtcgaaaa gggcttcgtc accgaggagg atttccggga
     4981 gttcacgttt accaatgccg cgcgtctgca cacgcgcaac aatccggact tcttcaaagg
     5041 cacggtcgtc gagcacgcgg tgggcgacga gctgggactg aaggttggtt gtcgttaatg
     5101 aagctgacag ctaagtgtta atggctcagg caaagacgta gatgaggcaa ggaagaagag
     5161 tgtttaatta ccgcaggtat cttggtgtaa ctctcgggcg gaggcgcttg tgatgcactt
     5221 atcgcggagt gaggaacgaa tgcgcggcct gtattgtctg cgccgctcgg tcctcttcgt
     5281 aacgacgata ttcacgctgt tcggtgatgc caatccggca gtaggcatac cgggcgcgga
     5341 cgacgatggc caaaaatgct acagggccac tcatcctgag gcagtcatac ggcattgtag
     5401 ccaaacgatt tcgtcggcaa agttcccgcc caaagcaatg gcaatggcct tctacaggcg
     5461 cgcgaatgcc tataaccaga agtcagaatc tgaccgggcg attcgagact acaacgaggc
     5521 gatccggcta aaccccggat atgccgatgc tttcgcgaat cgtggcgctg cctatgcgcg
     5581 caaaagagat tacgatcgcg cgattcaaga ctacgatgaa gcgattcgac tgaaccccag
     5641 acatgccgat gccttctcga accgtggcgt cgtctatgcg cgcaaaaaag attatgagcg
     5701 cgccatccaa gactatgatg aagcgatccg cctgaaccct gatcacatcg gtgcgttgta
     5761 tgctcgcggc aacgcgtatc ggcgtcgcgg cgattacgac caagccgtgc ggaactacga
     5821 ccaggtagtc cgcttgaatc cgaggcatgc tcaagcctac tccaatcggg gccttacgta
     5881 cgccgccaag gggcgcgaca atctggcgat tcaagactac gatgaagcga tccgcataaa
     5941 ccccgagcat gtgaatgctt actacaaccg aggaaatgca tacaggcgca cgggagctta
     6001 cggcaaggct gtggagaact atacccgagt gattcggctg aacccgaaac acggcaacgg
     6061 ctattccagt cgaggcatgg tgcgctttta tcagggggag ttcgcctcgg cagcggccga
     6121 tttctctgag gctcgccgat tcgcacccga caacttgtat cttgcgctct tactgcatgt
     6181 cgccgaagcc cgcgctggaa tccagggtca ggcggcgcaa actgaaatcg cgcgcagagg
     6241 ggatctcgag agctggcccg gtcccatcct tgccatgctt gtcggtaagc taccggagca
     6301 tgttatcctc accgctggta ccagctcgga tcaacctgag aagcgatgtc aggcttactt
     6361 ttatcttgga caaaggcggc tcatgcagca gaagaaaagt gaagcggccg aaatgtttcg
     6421 tcaagcagct gccacacaag cctcgactct ctttgaattc gaggcggcgg gagccgagct
     6481 cagccgtttg cgcaattaac ggcgatttca tcggcttccc cgaactcagt cgatcctcgg
     6541 tctatcgtta cagacaagtc taaagtccta ctcgtaaacg ccctacagcg ctcgctgtgc
     6601 ggagagccac ggtaacgagc ggggcaagaa actacaactt tgataccaat tttatcctcg
     6661 taggctgcaa atcgaagata aaaattgacg tgtgcaatct ttaagatagg atcggttcag
     6721 gcggtgaccc cagttagttt ggacgaattt acaggaggaa ggttatgttt attaaagcga
     6781 ttgcattggt cgccattatt ttgtttgcgc cggcgccatc gttagcccag aattccggga
     6841 gcgtgacatc ccttgagacc tttaaccttg ctgatattgc cgatgattca atcgtcgtgc
     6901 tcagtaaatc cgaatcgggg gtgcccgggg tggcgcacgt gaagtacgtg tatgtgaata
     6961 gtatttgttc gggaggactt cagctcgttg attattacgg cggggaattt gacgccaacg
     7021 atcgtaggct gcgccgtgat gagggtcagc gatgctcccg ggagtctcac gatactggtg
     7081 gagatgaaga ctagaattct atctgacagt ttgttgaggg gtcccgccag gttcctcaag
     7141 aaaaaccacg ctcgtcgtta tgtgggaaga ttagtttcaa ccgtctctat gcgtaagccg
     7201 gccccgaaac attccgtcgc aacgatatca gaacaaacag gtaagagcga cgactagcat
     7261 gggcatggcg tcttctagaa cgccatgtgt caaaccaccc agcaaacctt tctcgtcaaa
     7321 cgcggcaggc caggcgccac gaaacgccct aatagaagga gagcgcggat agaatgagag
     7381 atctttgcga tcgcggtcat gcggacgatg cgaatcaaga tagagcatta gataaaccga
     7441 tgaagccagt gaataattga gcggccaatc taaaattgaa cactacagcc aaggaggcga
     7501 gcaagccgaa aaaactgaaa tgagcgctgg gctaaagaca tcggggtagc agggcggcgt
     7561 acaggcagat aaatcgacca cacggcaagc gcaaatactc ccgtacgctc gacagcggat
     7621 ggtttgcgta gtggttgaac atacattcta atgagactac tgagcttgcg gccgatctca
     7681 aaacgagttc ggagactatt gcggttacta aaaaggcacg gtcgtggagc acgcggtggc
     7741 ccatgagctt ggactgcaaa cgcagttggc gcagcgacag attgctgaag gggatcatgg
     7801 ttttatcggc tcatcgggac cggagtgatg gaatgatgag tttaagatca atagttcaat
     7861 tccgattatt ccaactttca ttttgtctct ctggcgcctc tcgatccctg agcagcgtga
     7921 aaatcaagtt ttcagcaggc ggtaatcgga gaatatttta atttccgtta cattggattt
     7981 tgcggaatcg aaacaagaat acttttataa agcatctcct gagcggcgcg ccgggtcatt
     8041 gtgcgcgggc cgaactgaaa aacttcgttg catatgggcg ggaaattctt tagataggtg
     8101 agagtttcga tctgccagca attcccagcg atgaaattta gcgatttatc gatcgacgag
     8161 atccggcatc aatgtctcga tgggcagaag ccagtcagcc cgcagattct caacaaattg
     8221 cgtcgggatc cgcgtcaagg cgtgcgcaag ctcctcgaga ttctcaacag acgctacgag
     8281 caagagcgcg ccgagcgatt acggctgcaa aacatgctca atttcgagcg agtactgtgg
     8341 aagtccggca tccgcacggt tgcgggggtg gacgaagtcg gagtgggacc cttggccggg
     8401 ccggtggtgg ccgcggcggt ggtttttcca cccaacacag agcttgacgg agtcgatgat
     8461 tccaagcagc tcgaaccgga acagcgaact aatttggcgg cgaggattcg tgaacatgct
     8521 accgctatcg gcgtgggctc cgcggaggtc ggagagatcg atcgactgaa tatctatcat
     8581 gcggggctcc tcgctatgcg tcgagccgtc gaagctctgc cctgtatgcc cgagcatgtg
     8641 ctggtcgatg cgcgggtcat ccccggtgtt tccgttccgc agaactcttt caataaaggt
     8701 gatggaatca atttttctat cgctgccgca tcgatcatcg ccaagacgca tcgagaccgt
     8761 ttgatggaag atctcgaaaa agaatatcct ggttacggct tcgcccagca caaaggttac
     8821 agcacgccgg aacaccaaaa cgcgatccgc gagctcggac catgtccgat tcatcggctg
     8881 tcgtttcact tcatccgaga actttgcgga gagttctccg aactgttcta tactctcaag
     8941 cagcagcttc aggaagcgaa taccgccgcc gatttgcgcg gactcgaacg caagctgagc
     9001 gagcgctggt cggagctaga tgaaagggaa cagcgcaaaa taagattgat gctgtcgcgc
     9061 cgatggaaaa cggtttgatg cggcgatcgc caaagatcgg cgctgcgcgc ctcactcgga
     9121 gggacgtgat gcaaagtccc aagcagagaa ctgcgcttgc tagtcccgtt atattcttat
     9181 cgccaaagcc actccagcgc tccctcgaag ctcacggttg gtcacgggcc tgttgcaacg
     9241 atgatccgct gaggctcgag taccacgata gaaaagggaa gaagtgggaa cgataattct
     9301 tgagccaccg aggacagcgc cacattcgat gcgacggctt ctcgggtcgg tcgaagcgaa
     9361 ttcaagatat tccctcaatg ttagaacaac cgacgttatc gccgtgctct gctggcccat
     9421 cggttcaaac tcaaaagcgg cagcaagctc cacggcctcg gttctggcat cacatttgat
     9481 gggggcgaag catatcgtcc gtcgttaaca ttggtgccgc gttgatctcc tgaatgtctc
     9541 tgacgccagg tccgtaatgc ccagctctcg cgtagatcat aagagctttt ttttcctggc
     9601 aggaggtacg tttctcacgg ttcttagcta ctacgactcc gcaattaaca gtcctcagga
     9661 tgagaaacca tgatgagtct ggctcgttcg tgccgcatgc cgcttagtta acggtacatt
     9721 caaaatggct cgacttcgtg ttattcagcg gagaaaattg tcattgattc gcagcctgtt
     9781 aggcgcgagc gggtatacga cgttgttact tattgggaaa cattctgctc gctctcgacg
     9841 cttttcttaa ccgtcgggcg cttcatcatc cgctcctgcc atgccgtata ggcgctgagc
     9901 tctttcatgg gaaatccgct gcgctcgccc cagccataga agaccagcgc gtagggatcg
     9961 accgcggtga actcgcggcc catgacccaa tcgtttgctt caatcatcga gtcgatctcc
    10021 tggagattgg cccagaacag tttacgcccg ttttccttga ccccggcatg cgcggcttcg
    10081 ctttcggcga aacgctcagg tcgatgaaac cgctggtacg atggatgcac gacgcttgag
    10141 aaccagcaca tcgtcccgat gcagcgcgcc tcctcggccg gatccgccgg catcagccgt
    10201 ttttcgggaa aacgccgtgc gaggtaagtc aggattgccg tgttctcgac caggatcttg
    10261 ccgtcgacgt tgagtgccgg gacttttccc cgcgggttga tcttcaaata atcctcggtc
    10321 ctctgctgcc ctttggccag cagaatcggc ttgatctcgt acgtcgcgcc ggtttcttca
    10381 agaccgatat gagaggccaa agaacaagcg ccggggctga aatagagcgt catcatcgaa
    10441 acacctcctg agttatcgat caatccgagc agcgccccct gaacggcgtt acccggttat
    10501 gggcgacagt tagcgttaac ctacttatcc tgtcaatctt ctttcgctta cgagcgcgtt
    10561 actggtatgg aacagacaaa catcgctcat cgactacgcc gaagaagcct ctgcttcttc
    10621 catggcaaat gcttgcgcca ggagctcata agagcgatgg cggggagctg gatcataagt
    10681 ccatgtaacc accaccagtt cgtcgagctc caaatgtttc gcaagctcat ctaatcgcgc
    10741 ggcaacctgg tctttagatc cgacaatcgc tttgcggcga agcttctccg cagttctcgt
    10801 ctccgcaaca gaatacggcc gatccgcttc gtcgggaggc atgaggggta aacggatgcc
    10861 ctgtttgcga tcgataatcg cacgttcacg ggttttaaac tggtagagcg cttcttcttc
    10921 gctatcggcc gcgagcgccc agacgcaaat cgtcgccatc ggtttgggat gtttttcgct
    10981 gggttgaaaa ttgttgcggt aaagtttcag cgcctgctcc acaccggtgc cctcgctgaa
    11041 aaaatacgca aacgcgtagg gtaggccaaa ataggcggcg agttgagcgc catagtccga
    11101 gctgccgaga atccatatct cggggctgct cggtccagtg ggctgtgcaa caatcgtcct
    11161 gaacgggtgt ccctcgggca ggggaattcc cgagacccag tgtcgcagct cctgcacttg
    11221 acggggaaac tggtcaagca cattctgggg gttcggattg agcgcatatg aagtcatctg
    11281 gtccgagcct ggggcccgtc caactcccaa gtcgatgcga cccggcgcga tagcctccag
    11341 gacgcggaac tgttcggcaa ctttcagcgc ggaatagtgc ggcagcatga taccggcgct
    11401 gccgatgcgg atatgagaag tagtggcggc aatcgcagcc atcactatct ccggcgctgt
    11461 cccggcgata gtgccggaat tgtgatgctc cgacacccaa tagcgatgaa aacccagcgc
    11521 atcgcagcgc cgcgcaagtt ccagtgtctc acgaatcgca gtgtcctggg ttctatcttt
    11581 ggacgccgtt gattgatcga gtactgacaa tctcatgtgt gtccggttca agagtacttg
    11641 cagggaacca cgactcacca caaagaatgc gaagagcaca aagttaagaa caataagtat
    11701 tctgtcgtaa gcttcgtgtc cttcgtgctt cgtggtgaga tgaactcagt tcaacctgtg
    11761 aaccctcgcg tggaaggctc tgttcgttga atcagccaag ccgatcacca actctcaaat
    11821 taacctgctt ggcgaattcc gtgccgctga ttttgccggc ttcgccgcgc actcttttta
    11881 ctgttagcgt gcccccggtg accgcgatga tgatctcttc gctgccgatc ccggtaactt
    11941 ggcccggctc ggcgccgttt gcgtttttct gtaaccgtgc gtcgaagagg cgcaaagtct
    12001 tgccctggta agtagtgtgc gcgccgggtt ggggatcgca gccgcggatc aaattataaa
    12061 cttcctgggc cggtttggac caatcgatct tggcgtgttc atcgccgcac ggcggatcgt
    12121 aagtcgcctt ggactcatct tgcacaatcc gcggcggatt acctgcttta atcaagtcca
    12181 ccgcttcgcc g
//
LOCUS       DASUTG010000165         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1656468, whole genome shotgun sequence.
ACCESSION   DASUTG010000165 DASUTG010000000
VERSION     DASUTG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656468"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2483)
                     /locus_tag="VF089_03720"
     CDS             complement(<1..>2483)
                     /locus_tag="VF089_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6173098.1"
                     /translation="KIDDIPPVLVKTLLFLEDRDLDNPNFSRQNPAVEWDRFFKAALL
                     YLGSKLYLDVPVQGGSTLAVQLEKFRHSAHGRTDSPLDKFRQIVGASLKAYRAGPNTR
                     AWRDRIIVDYFNTVPLAAAPGYGEIHGLGEGLYAWFGMQLADAVRALQTPGITIPKVR
                     VYKHILTLLISVRAPSVFLVEERGSLEHKVNQFTQLLAREGIIDDDFAAAVEETPIQF
                     LTHGPVPPQPSSGRNKAANAIRMTMTGLLGVTNLYDLNRLHVEVESTIDVPLQKKVVE
                     FLQSLTLPETIKAYGLNGERLLENSDPKRVIYSFLLIEPTPQGNFVRVQADNLAAPFD
                     FNKSVKLELGSTAKLRTLTHYLELIAELHKELSPLGQKELAQKMRQARDPLTSWAMET
                     LKTEPSIALQPFLERAMERRYSASPFEAFFTGGGLHHFENFDRDDNGKILPLRDAFRN
                     STNLVFIRLMRDVVSYHRARLAYNADAVLSDPNNADRQAMLARIAEEESRAALRRAYQ
                     NFRGQTPEQITARLLGNKRNAERRLAILFFAWRIGADEQALASWLGENQYQATGEVVG
                     KLFRAYRNPRLTLVDYAYLLSLHPLDLWCASEFRNNSNLSWEELYTKSAEARRTGSAW
                     LLTPRNRKAQDLRLRIRFERDAFARMTPYWQRLGFPFKTMVPSYATALGNSSDRPVAL
                     AELIGILVNDGLRKRSLSLNKIHFARSTPYETVMEPEPRTGVQVLAPEVARTVRKAMA
                     DVVEHGTARRLNGVFRLADGQLLTVGGKTGSGDNKYETFNRYGGVISSRATNRTAAFV
                     FYIGDRYFGVITAYVQGREAADYRFTSAL"
BASE COUNT          539 a          680 c          719 g          545 t
ORIGIN      
        1 gtaacgcgct ggtaaagcga tagtccgcgg cctcgcgtcc ctgaacgtag gcggtgatca
       61 cgccgaagta acggtcgccg atgtaaaaga caaaagccgc ggtgcgattg gttgctcgcg
      121 aagaaatcac accgccgtag cgattaaaag tttcatactt gttgtcgcct gatccggttt
      181 tgccgccaac cgtgagcagt tggccatcgg caagcctgaa aacgccattg agacgccgtg
      241 cggtgccgtg ctcgaccaca tcggccatcg ccttccggac ggtacgagct acctccggtg
      301 ctagcacctg cacaccggtt cgcggctctg gttccatgac ggtttcgtag ggggtgctgc
      361 gggcgaagtg aatcttgttg aggctgaggc tgcgcttgcg taggccgtcg ttcaccagga
      421 ttccgatcag ttccgccaac gccaccggcc gatccgagga gttgcctaag gccgtagcgt
      481 aagacggcac catcgttttg aacggaaacc cgagccgttg ccaataagga gtcatgcgtg
      541 cgaacgcatc tcgctcgaaa cgtattctca gccgaagatc ctgcgccttg cggtttcgag
      601 gggtgagcag ccatgccgat cctgtgcgcc gggcctctgc gcttttggtg tagagctctt
      661 cccaggaaag atttgagttg ttgcgaaact cgctagcgca ccagagatcc agcggatgca
      721 acgataacag gtaagcgtaa tcgaccaacg ttagccgcgg gttgcgataa gcccggaaca
      781 gcttaccgac gacttcgccg gtggcctgat attggttttc gcccagccat gacgccagcg
      841 cctgctcatc tgcgccgatg cgccaggcaa agaaaaggat cgccagacgc cgttcggcat
      901 ttcgtttgtt ccctaggagg cgagcggtaa tctgttccgg agtttgaccg cgaaaattct
      961 gataggcgcg gcgcaaagca gcacgggatt cctcctcggc aattctcgcc agcatggcct
     1021 gtcgatccgc gttgttggga tcgctgagca cggcatcggc attgtatgca aggcgcgccc
     1081 ggtggtagct caccacgtct cgcatcagcc gaatgaagac taaattggtg gagttcctga
     1141 aagcatcgcg cagcggcaag atcttgccgt tgtcatcgcg gtcgaagttc tcgaaatggt
     1201 gcaaaccgcc gccggtaaag aatgcttcaa aggggctggc cgaatagcgg cgttccatgg
     1261 cgcgttcgag aaacggttgt agggcgatac tgggctcagt cttcaacgtt tccatcgccc
     1321 aactcgtgag tgggtcacga gcctggcgca ttttctgggc cagctctttt tgaccgagag
     1381 gagagagctc tttgtggagt tcggctatca gctcgaggta atgtgtcaaa gttcgcagtt
     1441 tggccgtgct tcccaattcc agtttgacac ttttgttaaa gtcgaaaggc gcggccaggt
     1501 tgtcggcctg cacccttaca aaatttccct gcggcgtcgg ctcgatcaga aggaaagaat
     1561 agatgacccg cttgggatcg gaattctcca acagccgttc accattcaat ccgtaagcct
     1621 tgatcgtctc gggaagcgta agagattgca gaaactcaac aacttttttt tgcaatggaa
     1681 cgtcgatggt gctctcgact tcgacatgca atcggttcag atcgtataaa ttcgtaacgc
     1741 ctaagagccc cgtcatcgtc atccgtatcg cgttggccgc cttgtttctc cccgaagaag
     1801 gctggggcgg taccggacca tgggtcaaaa actgtatcgg cgtttcttcg acggcagcgg
     1861 caaagtcgtc gtcgatgatc ccctcgcgcg ccagcaactg agtgaattgg ttcactttgt
     1921 gctcgagcga gccacgttcc tcgacgagga ataccgaggg cgcccgcacc gagatgagca
     1981 aagtcaaaat gtgcttatag acacggactt taggaatagt aatcccgggt gtttggagcg
     2041 cgcggacagc atctgcgagc tgcatgccga accaggcgta tagcccttct cccaagccgt
     2101 ggatttcgcc gtaaccgggc gctgcggcaa gcggaacggt attgaaataa tcaacgataa
     2161 tccgatctct ccaagcacgt gtgttggggc cggcgcggta agccttgagg ctggcgccga
     2221 cgatttgccg gaacttatcc agtggcgagt cggtgcggcc gtgcgccgaa tggcggaact
     2281 tctccaactg caccgctaac gtgctgccgc cttggacggg aacgtccaag tagagtttcg
     2341 aacccaaata cagtaatgcc gctttgaaga atcgatccca ctcgaccgcc gggttttggc
     2401 gcgagaaatt tggattatct agatcacgat cctcgagaaa aagcagcgtt ttaaccaaca
     2461 ccggcggaat atcatcgatc ttg
//
LOCUS       DASUTG010000166         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1953174, whole genome shotgun sequence.
ACCESSION   DASUTG010000166 DASUTG010000000
VERSION     DASUTG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953174"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VF089_03725"
     CDS             complement(<1..709)
                     /locus_tag="VF089_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173099.1"
                     /translation="MKRIFLLMLWLNATVYSPTIAYGAAAAETAGQILAKLNKLPPDQ
                     RQKVLIEKAKGEGQVSFYSSLQAQQIDPFIQLFRKRYPFINVNPYRVSGNRQVLRIQT
                     EMNAGNHLFDVTNGSAEQAAALKKIGAIDPYFSPQRDFFPQSSKDKDGFFSSLYVIPI
                     VLGYNTNLVKRPDAPKTYEDLLHPRWKSNMFLDDEAYEWYAVLLKHFGRDKGLQYMRN
                     LAKQDLRMVRGRTAQSQL"
     gene            981..1835
                     /locus_tag="VF089_03730"
     CDS             981..1835
                     /locus_tag="VF089_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6173100.1"
                     /translation="MDEIKPVKSGKVNVDGVNLYYELYGQGDPLVLIAGTGISLAPWR
                     IAQVPEFFKHYQVLIYDHRGLGRSDKPDVHYSTRLFAKDCAGLMDALGIKKAHVMGHS
                     MGGRVAQWLALDYPEKIRSLVLSGTGSGKYSELLEDYPRGVPLDAALEMIEKGYEKYQ
                     HDHWGPGFMFSEQFMKEHPDVVQKYQELIVDEVPPLKCYLRHVVARQCHETTDIVDRI
                     KAPTLVIVGSKDTHEGGTGSHVDSARALADKIAGSEFVLVEGGRHGYLREMPEKGHPP
                     ILDFLRRH"
     gene            2061..3347
                     /locus_tag="VF089_03735"
     CDS             2061..3347
                     /locus_tag="VF089_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6173101.1"
                     /translation="MNKQEIIDKQKKYLFSCVATYYDQPLVIDHAKDQYVYDADGKEF
                     LDFFGGILTVSVGHCNERVTKAVDEQTHKLQHMSTLYANEPQVQLAEKLAQITPGRLE
                     KSFFTNSGTEANETAVLLAQIHTKCQDVITLRHSYSGRSYLALSLSAQSSWRLTPNVV
                     PGVHHIANAYCYRCPFGLTYPSCNIKCATDIEEAIQTMTSQGRIAAFLAEPIQGVGGF
                     ITPPKEYFKEIVGIVKKYGGLFICDEVQTGWGRTGGKMFGIEHWGVEPDIMTFAKGMA
                     NGVPIGATIATPEVADSMQGNTISTFGGNPVTCTAAHATISVIEEDNLVENARVMGNR
                     LREGLNALKDKYPIIGDVRGMGLMQGMELVGEKKKPDAESTKRLMELTKNHGLLLGKG
                     GTYGNVVRISPALNVNKDQIDHALKVLDQSFSQLGL"
     gene            3397..>3544
                     /locus_tag="VF089_03740"
     CDS             3397..>3544
                     /locus_tag="VF089_03740"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6173102.1"
                     /translation="MNTLSRRILGAIPVLLGISFLVFMLMHIAPGDPVSLLLGDDATP
                     ADVEQ"
BASE COUNT          831 a          910 c          987 g          816 t
ORIGIN      
        1 acagttggct ctgggccgtg cgcccgcgca ccattctgag atcctgttta gccagatttc
       61 tcatgtactg aagtcctttg tcgcgaccaa agtgcttgag cagtaccgcg taccactcat
      121 aggcctcatc gtcgagaaac atgttgcttt tccacctagg gtggagcagg tcttcgtatg
      181 ttttcggcgc gtcagggcgt ttaaccaggt tggtgttgta gcccagaacg atcgggatga
      241 catatagcga ggaaaaaaat ccatctttat ctttgctcga ttgcggaaaa aaatcgcgct
      301 gcggtgaaaa gtaaggatcg atagcgccga ttttcttcag cgcagcggct tgttcggcgg
      361 aaccgttggt gacatcgaac agatggttgc cggcgttcat ctcggtttga attctcagga
      421 cctgccggtt acctgaaacg cggtaaggat tcacattgat gaaagggtag cgcttgcgga
      481 atagctggat gaaaggatcg atctgctgag cttgcaagga gctataaaac gacacctggc
      541 cctcgccttt ggctttctcg ataagaactt tttgccgctg gtccggcggc agcttgttca
      601 acttcgccag gatttgaccg gctgtctcgg cagcggcggc gccatacgca atcgttggcg
      661 aatagactgt cgcattgagc cacagcatca ggagaaaaat ccgcttcatg aatcgcctcc
      721 gtagtctgat ttagccggta ctgggaccgc ggcttccggt tatcgcacgc gtgatttgct
      781 gtcaagttga ttccagctcg gctccaacct aatttatttc gacatgacgg gtgcaagcgg
      841 acggacatga aactgtttac ttgagccgtg ctctataccc atctaagctt tgttgactct
      901 aatttctata ttgggtagcg tctccgtgcc tgtttacacc cataactctg ggttataggc
      961 gcaagcggaa ggaggctact atggatgaaa tcaagccggt caagagcggc aaggtaaatg
     1021 tggatggcgt caacttatat tacgagcttt atggtcaagg agatccgctg gtgctgattg
     1081 ccggcacagg catcagccta gcgccgtggc gcatcgctca ggtgccggag ttcttcaaac
     1141 attatcaggt gctgatttac gatcatcgcg gtttgggccg cagcgataaa ccggacgttc
     1201 actactctac ccggcttttc gccaaagact gtgccggttt gatggatgcg ctgggtatca
     1261 aaaaggcgca cgtcatggga cattccatgg gcggccgcgt tgctcagtgg ctggcgttgg
     1321 attatccgga aaaaatacgc agccttgtgt tatccgggac cggttcggga aagtacagcg
     1381 agctgctcga agactatccg cggggcgtgc ccctggatgc ggccctggaa atgattgaaa
     1441 agggttatga gaaatatcag catgatcact ggggtcccgg ttttatgttc agcgagcaat
     1501 ttatgaaaga gcatccggac gtcgtgcaaa aatatcagga gctgatcgtc gacgaagttc
     1561 cgcctttgaa gtgctatttg cggcatgtgg tcgcgcgaca gtgtcacgag accaccgaca
     1621 tcgtcgacag aatcaaggcg ccgactctgg tcattgtcgg cagcaaagat acgcatgaag
     1681 gcggcaccgg cagtcatgtt gattcggcca gagcgctggc ggacaaaatt gccggttccg
     1741 agtttgttct ggtcgagggc ggcaggcacg gttatctgcg agaaatgccg gaaaaggggc
     1801 atccgccgat tctcgatttc ctgcgacgcc attaacaggt tgacaacagg ttgaccaaaa
     1861 aggctcacat gcttcgttgc gctcgatcgc ctcacgtcaa cgtactgaaa cagtacgcct
     1921 ccggttcgtc gactcttcgc gcgcctagcc tctgagacct ttttgatcaa cctgtgccaa
     1981 actcctacga gcgataacaa ggctgctgcg cagagacatc tttcggaatg tagataacgt
     2041 aatgggagct aggtgacacg gtgaacaaac aagagattat cgacaaacag aaaaagtatc
     2101 ttttttcttg cgtcgccacc tattatgacc agccgctggt gatcgatcat gcgaaagatc
     2161 aatacgtcta tgatgccgac ggcaaagaat tcctggattt ctttggcggc attctcaccg
     2221 tcagcgtcgg ccactgcaac gagcgggtca cgaaagctgt cgatgaacaa actcacaagc
     2281 tacagcacat gtcgaccttg tatgccaatg agccgcaggt tcagctggcg gagaagctgg
     2341 cccagatcac gcccggcagg ttagaaaaat cgttttttac caatagcggc acggaagcca
     2401 acgagaccgc ggtattgctg gcgcagatcc ataccaaatg ccaggacgtc atcaccctgc
     2461 gccatagtta cagcggccgt tcctatctgg cgctcagcct ctcggcgcaa tcgagctggc
     2521 ggctgacgcc gaacgtcgtg cccggcgtgc accatatcgc taatgcctac tgttaccgtt
     2581 gtcccttcgg actgacctat ccgagctgca atatcaaatg cgcgaccgac atcgaagaag
     2641 cgattcaaac catgacatcg caggggcgta tcgccgcttt cttggccgag ccgattcaag
     2701 gcgtcggcgg ttttatcact ccgccgaaag agtatttcaa agagattgtc ggcatcgtca
     2761 agaaatacgg cgggcttttc atttgtgacg aggtccagac cgggtggggc cgcaccggcg
     2821 gcaagatgtt cggcatcgaa cattggggcg tcgaacccga catcatgacc ttcgccaaag
     2881 gcatggccaa cggcgtgccc atcggagcga ccattgcaac cccggaagtg gccgacagca
     2941 tgcaaggcaa cacgatttcc actttcggcg gcaatccggt cacatgcacc gccgcccatg
     3001 ccacgatcag cgtgattgaa gaagacaacc tggtggagaa tgcccgtgtg atgggcaacc
     3061 gtttgcgcga aggtttgaat gcgctaaagg acaaataccc gatcatcggg gatgtgcggg
     3121 gcatgggctt gatgcaaggg atggagcttg tcggcgagaa gaaaaaaccg gatgccgaaa
     3181 gcacgaagcg gctcatggag ttgaccaaga accatggctt attgctgggc aaaggcggaa
     3241 cttatggcaa cgtcgtacgg atatcacctg ccctgaacgt caataaggac cagatcgatc
     3301 atgcgctcaa agtgctggat caatcttttt cccagttggg gctttaatcg tctgccggtg
     3361 aaatttccaa cgctctaatt ccgctgtttt aaaagaatga acactctgag ccgcagaata
     3421 ctgggtgcga taccggtttt gctcggtatc tcgttcctgg ttttcatgct catgcatatc
     3481 gctcctggag atccggtgtc gttgctcctc ggcgatgatg ccacgccggc ggatgtcgaa
     3541 caga
//
LOCUS       DASUTG010000167         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1360906, whole genome shotgun sequence.
ACCESSION   DASUTG010000167 DASUTG010000000
VERSION     DASUTG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360906"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VF089_03745"
     CDS             complement(<1..364)
                     /locus_tag="VF089_03745"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6173103.1"
                     /translation="MSERVYNVLFLCTGNSARSILAEGILAKEGAGRFLAFSAGSQPK
                     GEVNPFALKVLESFDYPTTGFRSKNWEEFSGSDAPVMDFVFTVCDSAAGEVCPIWPGQ
                     PMTAHWGIEDPAAVQGTDI"
     gene            complement(357..719)
                     /locus_tag="VF089_03750"
     CDS             complement(357..719)
                     /locus_tag="VF089_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6173104.1"
                     /translation="MESSPVISIFAALAQATRLEVFRLLIAHEPDGLAAGDIAKQLDV
                     PHNTMSTHLAILSRAGLIQAKRHSRSIIYRAQLDAVSALVCYLLNDCCGGRPEICAPV
                     LAEISPCCPPQTKKVVHE"
     gene            951..1319
                     /locus_tag="VF089_03755"
     CDS             951..1319
                     /locus_tag="VF089_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007793831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX6173105.1"
                     /translation="MANYPHIGLRVFLGPDSVLCSTKAGLLQSISETGSIAAAGRRMG
                     MSYKRAWYLIDTLNAYFNEPVVVSSKGGPKGGGASLTNTGEQVLACYLGLQRKSEAAA
                     SADLKQLASLSGPSRAQKAI"
     gene            1437..2261
                     /locus_tag="VF089_03760"
     CDS             1437..2261
                     /locus_tag="VF089_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6173106.1"
                     /translation="MSSLRFPHSYPAQSARHFIPVFLGGAVIGALGGVIGLGGAEFRL
                     PLLIGLFGFPALEAIILNKAMSLVVVASALPFRAATVPIGAIVENWSVIVNLLAGSLF
                     GAWFGAGWAMRMKSELLHKIIAVLLLVIAMVLVFGHEVAGTGQPIVIGLYQTVAGVVA
                     GVGIGLVASLLGVAGGELLIPTLILLFGVDIKLAGSLSLAVSLPTMLIGFARYSRDQS
                     FSVIARNYPFAWIMALGSVLGTFIGGQLLGIIPGEMLLPLLAFILLVSSVKVWLHR"
     gene            complement(2854..>3381)
                     /locus_tag="VF089_03765"
     CDS             complement(2854..>3381)
                     /locus_tag="VF089_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6173107.1"
                     /translation="AFMRHLNPLRRKRWFVYSKPPFAGPRAVLAYLARYTHRVAISNR
                     RLIRADADSVTFKVKNYRIEGPGRYSTMTLATPEFIRRFLIHVLPKGLHRIRYYGLLA
                     NGNRAANLARMRALLDMSAPAPEPRNPNDTATEPRFAPCPCCGGRMRILLITEAGLAP
                     SAGATPPAVIRIDTS"
BASE COUNT          619 a          954 c         1093 g          715 t
ORIGIN      
        1 cgatgtcggt gccctgaacg gccgcagggt cctcgatgcc ccaatgcgcg gtcatcggct
       61 gaccaggcca tattgggcac acctcgccag cggcgctgtc acagacggtg aagacgaaat
      121 ccatgactgg cgcgtcggag ccgctgaact cttcccaatt tttggatcgg aagcctgtgg
      181 tcggatagtc gaagctctcc agcaccttga gagcgaatgg gttcacctca cccttcggct
      241 ggctgccggc agaaaatgcc aggaagcggc cggcgccctc cttagccagg atgccctccg
      301 ctaggatcga tctggctgaa ttgcccgtgc ataggaacag gacgttataa acacgctcac
      361 tcatgaacga ccttcttcgt ttgaggtgga cagcagggag agatctcggc gagtaccggc
      421 gcgcagattt cagggcggcc gccacagcaa tcgttcagga ggtagcaaac caacgcgctc
      481 acggcgtcga gctgggcgcg gtagatgatc gaccggctat gccgtttggc ctggatgaga
      541 ccggcccgcg acaggatcgc taagtgcgtc gacatcgtgt tgtgcgggac atctaactgc
      601 ttagcgatgt caccggcagc taaaccatca ggctcgtgcg cgatgagcaa gcgaaacacc
      661 tcaagtcggg tcgcctgtgc aagggcagca aaaatcgata tcacggggct tgattccata
      721 tgtcgatgat aatcgacata tggacgcaat gcaagaacgc cacgctttgc acggaaggta
      781 ctcgctgaac agcggaatac cgcaatgggc aaaacgcacg gctcttggcc caacgcggag
      841 ttgggccgcg cgcgtttgta agtttcgcca agatgcctgt gattgcaggc gccttgatct
      901 gtcgacctaa ccacgagtaa acgttggtgg aacgttaggg aggcaggtca ttggcgaact
      961 atccgcacat cgggctgcga gtcttcctcg ggcctgacag cgttctctgc tcaacgaagg
     1021 caggactgtt gcagagcatc agcgagaccg ggtcgatcgc ggcggcgggc cgtcgaatgg
     1081 gaatgagcta caagcgcgcc tggtatctca ttgatacatt gaacgcctac ttcaacgagc
     1141 cggtcgtggt gtccagcaaa ggcgggccga aaggtggtgg cgcttcgctc accaataccg
     1201 gcgagcaagt cttagcctgc tatcttggct tgcaacgcaa atccgaagct gccgcctctg
     1261 ctgatctaaa gcagcttgcc tcgctgtcag ggccttccag agcgcaaaag gcgatatagc
     1321 ttcagcgcta tatcgttgct tgcagaccga cgcatcgata tagtccgccg gctatatcga
     1381 tgggagttgc gcatctagcg atatagcttc tctgctatat cgctagtgag gccgacatgt
     1441 cgtctctccg tttcccacac agctacccgg cccaatcggc ccggcatttc attccggtgt
     1501 tcctcggcgg tgccgttatc ggcgcactcg gtggggtgat cggcctcggt ggtgctgagt
     1561 tccgcttgcc gctcttgatt ggcttgttcg gatttccggc gttggaagcg atcattctca
     1621 ataaggccat gagcctcgtc gttgtggctt cggctttgcc tttcagggcc gccacggtgc
     1681 ctatcggcgc catcgtcgaa aattggtcgg tgatcgtaaa cctgctcgcc ggtagcctgt
     1741 tcggggcatg gttcggggca ggctgggcaa tgcgcatgaa atcggaatta ctccataaaa
     1801 tcattgccgt tttattgctg gtcatcgcga tggtgctggt cttcggccat gaggtggcgg
     1861 gaacgggaca gccaatcgta atcggcctct accagacggt tgccggtgtc gtggcggggg
     1921 taggcattgg tctggttgcg tctctgctcg gggtcgccgg cggcgagttg ctgatcccta
     1981 cgctaatcct tctattcggc gtcgatataa agcttgctgg aagtctatcc ctggccgtca
     2041 gcctgccgac catgttgatt ggttttgccc gctacagccg tgatcaaagc ttcagcgtca
     2101 tcgcccgcaa ctatccattc gcttggatca tggcattagg gtcggtcctg ggaacgttca
     2161 taggcggcca gcttctcggg atcattccag gggagatgct attgccgctc cttgccttca
     2221 tcctgctcgt gtcatcggtg aaggtgtggc ttcaccggta gcagtccgcc ttgcgcttac
     2281 gcctatttaa gctgcgggag ggctcgctcc cacttgcgga ctgcggctcg gtcggcagat
     2341 ggtgcccctt ggcccgctca tgtcggggag ccgagagtgt ccggaaaggg cgcatcaaag
     2401 ccgctgtagg tatcggccga tttaggtttt gaaccggact tctcctctgc cgaccttcgt
     2461 agtcgagtcg ctcgcaatcc acgcgatctc aagattccgg attcgtgaag ggttgcggat
     2521 gccggccatg acgcacacgg cgtcacccgg tgggccggcg tccgcaagtc gctgatgaag
     2581 gaatccgcgg gaagtcgggc tatgcgcggc cgcgatctct atggggattt ggccgtcgcg
     2641 ggcagtggcg ggagcgtgcg atcaggcgca tcggagagct gccgagatac ctgatgggcc
     2701 gacaacgccg ccgccggtcg cgctctcgga gcgcctctcg ccggttgagg agcttgatat
     2761 taccgtttgg gcgcgcagac agacaagccc gcccgtggct gggccggcca gcagcgggcg
     2821 cgcggcagtt tgggatgagc cgtctttctc ctctcatgag gtatcgatcc ggatcacggc
     2881 aggtggggtt gcgccagcgc tcggcgcaag gccggcttcg gtgatcagca ggatgcgcat
     2941 tcgtccgcca cagcatgggc atggtgcaaa acgtggctct gtcgccgtgt cgttgggatt
     3001 gcgaggctcc ggggcgggcg cggacatgtc gagcaacgca cgcatgcggg cgagattggc
     3061 ggcgcggttg ccgttggcga gcaggccata atagcggatg cggtgcaagc ccttgggcag
     3121 cacatggatg aggaagcgcc ggatgaactc gggcgtcgcc agcgtcatgg tgctgtatcg
     3181 gccagggccc tcgatccggt agttcttgac cttgaaggtg acactgtccg catcggcgcg
     3241 gatcagccgg cggttggaga tggcgacgcg gtgggtgtag cgggcaagat aggccagcac
     3301 cgccctcggt ccggcgaagg gcggtttgct gtagacgaac catcgcttgc gccgcagcgg
     3361 gttgaggtgc cgcatgaagg c
//
LOCUS       DASUTG010000168         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1211889, whole genome shotgun sequence.
ACCESSION   DASUTG010000168 DASUTG010000000
VERSION     DASUTG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211889"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..1559
                     /locus_tag="VF089_03770"
     CDS             477..1559
                     /locus_tag="VF089_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173108.1"
                     /translation="MGIIERTYGKETNAAINEVLISSDSHVIEPAGLWQKQLPKAFQD
                     RAPDWGGQRPNDQPGGAQDKNKRVSEMAADGVSGEVLYPTHGLKCLSLDDHELESACC
                     RVYNDWLIDYCSAAPERLIGIAMLSMYDVDEAVKELERCRKAGMRGSVIWQVPPADLP
                     FSSSHYDKFWAASQDLEMPVNLHILSGHGYSRSRSFGLGDTPTGMTAEHNSVNMKLFQ
                     SVDSLYDLIYSGVLERFPKLKVVLVENELGWIPFVLEQWDYYFKRHGAKREGLLIKKL
                     PSEYFHNQIYTTFFNDAVGGHILSWWGQDNCMWSNDFPHGNTTWPHSRKVVARDLGDL
                     PPGLRAKLVRENVAHLYNIPVPTPIQ"
     gene            2027..2986
                     /locus_tag="VF089_03775"
     CDS             2027..2986
                     /locus_tag="VF089_03775"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173109.1"
                     /translation="MKSILFAALMLVPSLPVQAPAAEKTWRIGFLSPAIRDSFHDSFL
                     KGLRELGYIEGRNFILQPRLAAATQRRLTEAALELAKLRVDVIVARGTQATLAAKKAT
                     ATIPIIMTGSSDPVGTGLVASLARPDANVTGMSIVAPDLAEKRLELLKRVVTGSRFGV
                     LWNPTNAGNINEWEQSKAAANRLQITLLAREVRQPKDIYRAFKSLRPRTIDAVVTLTD
                     ALLSTGRTDIVQFATNNRLPGIYHLVDFVEQGGLMSYGPDLHHSFYRAAFYVDRILKG
                     AHPATIPVEQPIRFELAVNLGTAKQIGVTIPTNVLALADKVIK"
     gene            complement(3182..3574)
                     /locus_tag="VF089_03780"
     CDS             complement(3182..3574)
                     /locus_tag="VF089_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173110.1"
                     /translation="MFSRALAIWLLLLILALLNGAIREILLKPRFGEQAGHIVSTAIL
                     CAAIILLAWFSIAWIGPKNGREALVVGIAWVALTVAFEFLAGHYVFGNSWERLFADYN
                     VFRGRIWILVLVANLFAPLWAFLQKQPA"
     gene            3819..>4980
                     /locus_tag="VF089_03785"
     CDS             3819..>4980
                     /locus_tag="VF089_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019854126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX6173111.1"
                     /translation="MRRSTDRILTTHVGSLPRPADLREMWSKQTSGSAEDALQARLRS
                     AVSEVVLAQKTAGIDIPNDGEFGKPMRAASDRGAWGNYIFNRVSGFVPTPPEAVAPDT
                     AAPGAPMRIVGVRWEQREFAEFYADTGLGAPSTAASRPMCAAPIAYTGHEALRRDLAN
                     LRVAMDQAEVEEAFVASIAPGSLEMFCRGQNSYYPTAEAFIESIAAAMREEYRAIIDA
                     GFILQLDDPGLPGAWDMLDPRPRVDEYRRYAMLRVEALNRALEGIPEDRVRYHICWGS
                     WHGPHTTDIPLRDIADVMLKVRAQAYLTEAGNVRHEQEYKVWRDIKLPAGKILIPGVV
                     SHATPVVEHPELVADRIIAFANIVGRENVIAGTDCGLGGRVHPQIAWAKLRAL"
BASE COUNT         1159 a         1404 c         1364 g         1053 t
ORIGIN      
        1 gggcactgaa ggtaacgaac tcgccgaccg catggccatg ctcgccgcgc aacggaaaga
       61 aaaagaactg cggctgtatc gggaaaccat ggatattccg actctgctca agatgcgcgc
      121 gggatgatca gcaaaaaagc aacattttga ccggttgaac ggttttgcga ttcgactgct
      181 ttaggaagac agctcgatct acttaaaggt cgacgagctg aacggaaact gtatttggca
      241 agtattcgca cctgaagatt tctatcgagc cccgaccgat tctcgatgca tgatcaatga
      301 acactggaat gttaaaacgc caggccaaag ctccacccga ttgcctgcgg ggtcactgcc
      361 ctcgcttgca ccatggccct cgactgggtt atgtccatct agtgagcccg tcaccaactt
      421 tcaagaaatt tacatgagag ctacgtcccg acctaaccct acaaggagca aagatcatgg
      481 gtataattga acgaacctat ggaaaagaga ctaatgccgc tatcaatgaa gtgttgatct
      541 catcggactc ccacgtcatc gagcccgccg ggctttggca aaaacaactg ccgaaagctt
      601 ttcaggatcg cgccccggat tggggcgggc aaagacccaa cgaccagccc ggcggcgccc
      661 aggacaagaa caagcgcgtt tccgagatgg ccgcggacgg cgtcagcggc gaagtgcttt
      721 atccgaccca tggcttgaag tgcttaagcc tcgatgatca cgagttagaa tcagcctgct
      781 gtcgggtcta taacgactgg ttgatcgact actgcagcgc tgcgcccgaa cggcttatcg
      841 gtatcgccat gctgtcgatg tacgacgtcg acgaagcggt aaaggaactg gagcgctgcc
      901 gcaaagccgg catgcgcggc tcggtaatct ggcaggtacc gccggcagat ctcccgttct
      961 cctcatcgca ttacgataag ttctgggctg cctctcagga tctcgagatg ccggtcaact
     1021 tgcacatctt gagcggccac ggttacagcc gaagccgttc ttttggcctg ggagataccc
     1081 cgacgggcat gaccgcggag cacaatagcg tcaacatgaa actcttccag tcggtcgata
     1141 gcctctacga cttgatctac tccggcgtgc tcgaacgttt ccccaaactc aaagttgttc
     1201 tcgtcgaaaa cgagctgggc tggataccgt ttgtcctaga gcagtgggat tattacttca
     1261 aacgccacgg cgcgaagcgc gagggcctgc tcatcaagaa acttcccagc gaatacttcc
     1321 acaaccagat ctacacgaca ttcttcaatg acgccgtcgg cggtcacatt ctgtcgtggt
     1381 ggggtcaaga caactgcatg tggtccaatg attttccgca tggcaacacc acttggcccc
     1441 attcacgcaa agtcgtagcc agggacttag gcgatctacc tccgggattg cgcgccaaac
     1501 tggtgcggga aaacgtcgcg cacctgtaca atattcctgt tccaacgccg attcagtaat
     1561 cggcgtaacc acaggaatgc tgcagatcac ttccgcgcca gtgcggtcgc ccgcgaccgc
     1621 actggcgctt ctccagtttg cttgtacccg gcatttttag atttctcgaa agcctgagac
     1681 ggctggtgct cggctaacca gatccggcgc cgctgaatgc gggcgagatt tggcgaataa
     1741 cttcaatgtc gaaattccta tcgattgtgc tagtgcagtg cgcgtctctc tcagcgtccg
     1801 tctcccaaag tgctgtgcac cctcaccgct tgaaggaaaa tcgcgcgggt tgtatgttcc
     1861 tctaaactga acgattcgca ataatgtgac tcgcgtttca agcctcgaga aaccggcagc
     1921 gttaaagcca tgtatatgat cggttagcag cgcttttctt ccccctaaca agcttgacct
     1981 ctatcagttg aacagcacgg gtatgaaact gcgtcggcgt tttgccttga aatcaatttt
     2041 atttgccgca ttgatgttag ttccgtcctt gccggttcaa gcaccggcgg ccgagaaaac
     2101 ctggcgcata gggtttcttt caccggcgat aagggactct tttcatgatt cgtttctgaa
     2161 gggactcaga gaactaggtt acatcgaagg ccgcaatttc atcctccagc cccggctcgc
     2221 ggcggcgact cagaggcggc ttacggaagc agcccttgag ttggcgaaac ttcgggtcga
     2281 tgtcatcgtc gcacgcggga cgcaagccac gcttgcggca aagaaagcga ccgcaacgat
     2341 tccaatcatc atgacgggga gctcggatcc ggtcggaacc ggactggtgg cgagtcttgc
     2401 tcggcccgac gccaatgtca caggcatgag cattgtggcg ccggatctgg cagagaaacg
     2461 gctggagctg ctcaagcgtg ttgtaaccgg ttcacgtttc ggcgttctat ggaatccgac
     2521 taatgcggga aacatcaatg agtgggaaca gagcaaagct gcggcaaatc gactgcagat
     2581 aaccttgctt gcgcgcgagg tgcgccagcc caaagacatc tatcgagcat tcaaatcact
     2641 gagaccccgg accatcgatg ccgtcgtgac tctcacagat gcgctgttga gcaccggacg
     2701 caccgatatc gttcagtttg cgaccaacaa tcgtctcccg ggtatttatc acctcgttga
     2761 tttcgtcgag caaggcggtc tcatgagcta cggcccagat ctgcaccatt ccttctaccg
     2821 cgccgcgttc tacgtcgatc gtattcttaa gggcgcccat ccggccacta tcccggttga
     2881 acagccgatc aggttcgagc tcgcggtcaa ccttggaaca gcgaagcaga tcggtgtcac
     2941 gattcccacc aacgtgctag cgctggcgga caaagtaatc aaatgattaa gcgagttgaa
     3001 cgttggagcc tagcaggctg ctgaaaaacc catatacttc gttgacgctc aatcgcctcg
     3061 cacctggatc tttttgagca gcctgcaaga gggctttttc agcaccctgc taggcctcct
     3121 ggagctagac agtagccaga cagccgattc gccgagcgat cggtcgtcat ctattttttc
     3181 attaggccgg ctgcttctgc aaaaaagccc agagcggcgc aaacaggtta gcgacgagca
     3241 caaggatcca aatgcggccg cgaaacacgt tgtaatcggc aaacaatctc tcccagctgt
     3301 tgccgaaaac ataatgaccc gcaagaaatt caaaagcgac ggtgagagcg acccacgcta
     3361 ttcctaccac caaagcctcg cgtccgttct tcggtccgat ccacgctatc gagaaccagg
     3421 caagaagaat aattgctgcg cacaaaatcg ccgtgcttac gatatgccct gcctgctcac
     3481 cgaagcgtgg tttaagtaag atttcgcgaa tggcgccgtt caacagagcg aggatcagca
     3541 gcaaaagcca aattgcaaga gctctggaaa acatagattt ctctcccgtg cgcgtcggat
     3601 caggtctttt atcgtgtacc aatcgtgaag tgactagtcg cctgatgact tgatagtgta
     3661 ggcccaaaga ttggccagtt ctatctgact ctgaagtatt tcaccgatga aaatcgaact
     3721 tgaatataca gactgtcgtg tgcttgagat aagcttgtat ttagcgataa ctcaatacaa
     3781 aagcctgacc gttacgactc aactggaggt atcgaaaaat gcgacgaagc actgatcgaa
     3841 ttttgaccac gcatgtcggc agtcttcccc gacccgccga cctgcgcgaa atgtggtcga
     3901 agcaaacgag cggttcggca gaagacgcgc tgcaggcgcg cttgcggtcc gcggtctcag
     3961 aggttgtgct cgctcaaaag acggccggca tcgatattcc caacgatggc gagttcggca
     4021 agccaatgcg cgccgcatcg gatcgcggtg cgtggggcaa ttacatcttc aaccgcgtct
     4081 ctgggttcgt cccgactcca cccgaagcgg ttgcgccgga tactgcggcg ccgggagcgc
     4141 cgatgcgcat cgttggagtg cgctgggagc aacgggagtt tgccgagttt tacgccgaca
     4201 cgggactggg agcgccaagc accgctgcca gccggccgat gtgcgcggct ccgatcgcct
     4261 ataccggaca tgaagcgctg cgccgggacc ttgctaacct gcgagtggcc atggaccaag
     4321 ccgaagttga agaggcattc gtcgcgtcca tcgctcccgg cagcctggag atgttctgcc
     4381 gtggtcagaa cagctactac cccacagctg aggcattcat cgaatcgatc gccgccgcta
     4441 tgcgcgaaga gtatcgcgcg atcatagacg ccggtttcat cttgcaactc gatgaccccg
     4501 gtttgcctgg cgcctgggat atgttggacc cgcggccaag ggtcgacgag tacaggcgct
     4561 atgcgatgct gcgggttgaa gccctgaacc gcgcgttgga aggtattccg gaggatcgag
     4621 ttcgctatca catttgctgg ggcagctggc atggcccgca tacgacggac atccccttgc
     4681 gggacattgc cgatgtgatg ctcaaggtga gagcgcaggc ctacttgacc gaagctggca
     4741 acgtgcgtca cgagcaggaa tacaaagtgt ggcgagacat caaattgccg gccgggaaga
     4801 tcctcattcc cggtgtggtg agccacgcaa cgcccgtcgt cgaacatccc gagctggtgg
     4861 cggatcggat catagccttc gccaatatcg taggccgcga gaatgttata gccggaaccg
     4921 attgcgggct cggcggccgc gtgcacccgc agatcgcatg ggcaaagctg cgcgcgctat
//
LOCUS       DASUTG010000169         4429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_471012, whole genome shotgun sequence.
ACCESSION   DASUTG010000169 DASUTG010000000
VERSION     DASUTG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471012"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..535)
                     /locus_tag="VF089_03790"
     CDS             complement(125..535)
                     /locus_tag="VF089_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX6173112.1"
                     /translation="MNQKCTQETPAQVRIDKWLWAAHFFKTRSLAAAAVAGGKIAPNG
                     ARPKPSRTIRAGDHLNIRRGAYKWIIFVKGTSTYRVPAPDAQALYEENEESRSRRQAA
                     SAQLKLERPDDFDSPGRPSKKDRRAISKFTNRRW"
     gene            complement(544..1296)
                     /gene="surE"
                     /locus_tag="VF089_03795"
     CDS             complement(544..1296)
                     /gene="surE"
                     /locus_tag="VF089_03795"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6173113.1"
                     /translation="MIILISNDDGIQSEGIVALEEALRPVGEIFTVAPDRAQSSMSHA
                     LTLHRPLRVQEVAPRRLAVNGTPSDCVKLALTGLLPVRPHLVVSGINKGPNLGDDIIY
                     SGTVSAAIEGALLAVPSIAVSLVTFRDFDFRAAAEFTAFLVSSIQQAGGIPPETLLNV
                     NVPPLPKSELRGWRLTRMGKRHYSENIIERVDPRGGKYYWIGGDDLGFDKEDGTDCVA
                     VHEGFVSVTPLQVDLTNYRVLENNTLPNFVWP"
     gene            1411..2661
                     /locus_tag="VF089_03800"
     CDS             1411..2661
                     /locus_tag="VF089_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX6173114.1"
                     /translation="MANAEIVAIGSELLLGQIVDTNSAWMAQRLTALGVNLYFTSVVG
                     DNPGRMREVIERALERADIVITSGGLGPTQDDLTREIVAEVTGRKLILDGGLLEQVEE
                     HFRRRGRTMTPNNRRQGYMPEGAIPIKNPNGTAPSFIVEDPRGIVFSLPGVPVELKWL
                     FENEVEPYLRRKFNLAEVIHYRVLKIVGIGESAVDDKIGYLIANSSNPTVGVLALPGQ
                     VDVRIAAKAANRDQAMNLIAPVEAEVRALLGNAIFAADQETMEDVVGRLLRAGNKTVA
                     VYEDLTCGSLSERLQAASTESFGAGVICNSESATRTLLTHGRHPAQTNDLIQDPAALT
                     DELAWSVRHQAACDFGLALHAVTDPDTTVQNLARGQSYVALTDGEKFIRRLSTSAGRG
                     QYDRSRMTLNALDLLRTALLEGMG"
     gene            complement(2773..>4429)
                     /locus_tag="VF089_03805"
     CDS             complement(2773..>4429)
                     /locus_tag="VF089_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX6173115.1"
                     /translation="ASWERILLRNEDILQRISHLGSYIGCLASSDARNESYLKEEAEL
                     VGMRAELGKLRIEFLRAIKETEEETFTAFVARPALDGAQNYLNRMRQEARRAMTPEKE
                     ILATDLGVDGIQAWGRLYDTISSKLEFDMIFPHGNRQRLPISQRRSLLEDPDRRIRKA
                     AFEGGNAAWQSIEDMAAAALNAIAGTRLTLNRHRRVDHFLEIALFQAAITRNTLDAMF
                     EALFSRFEVPRKILRLKAKLLARPSLAWFDVGAPLDLPGQEKLSWENATSLVGKSFER
                     AYPALGRFFEQQVVGKNWVDWEPRTGKRPGGFCTTSMLTKESRIFMTYNQSLGDVLTL
                     AHESGHAYHGAMMRDIRPYGRGYPMTLAETASTFGEEVLMNGILDDPNISQAQKALIL
                     DIETGHGAVYLLDIPVRYEFEKAFYEERAAGPLSVSRLKELMVDTQRKVFGDVLEVGG
                     EDPYFWASKLHFYITGITFYNFPYTFGYLLSRALYRMFKKNPSGFLPKYEEFLRLAGT
                     EPAEKLVQQTIGGDIEQPQFWAEAIDSLEEPSARLEMLLAEIL"
BASE COUNT          985 a         1185 c         1240 g         1019 t
ORIGIN      
        1 tgaatatttt tcagtgccta acggtaatcg tgtagcctga gcgttggact ttaaacaaaa
       61 agatagctct gtctatgact atcatccgat gacgcactat ttattccagg ttatatacct
      121 caggtcacca tctgcgattg gtgaatttcg atatcgcgcg ccgatccttt ttggaaggtc
      181 gccctgggct atcaaagtcg tctggccgtt caagtttaag ttgggccgag gcggcttgcc
      241 ttcttgatct gctttcttcg ttttcttcgt agagtgcttg agcgtcgggg gccgggactc
      301 ggtaagtgga ggttcctttg acgaaaatga tccatttgta agcgccgcga cggatgttga
      361 ggtgatcgcc ggcgcggatc gttcggctcg gcttgggacg ggctccgttg ggcgcgattt
      421 tgccgcccgc tacagccgca gcggccagcg aacgtgtttt gaaaaaatgc gccgcccaaa
      481 gccacttgtc gattctgact tgagccgggg tttcttgggt acacttttgg ttcacggcac
      541 acgctagggc catacgaaat taggtagagt attgttctcg agcacccgat aatttgtcag
      601 atccacctgt agcggagtca ccgaaacaaa tccctcatga actgccacgc aatcggtgcc
      661 gtcttctttg tcaaaaccga gatcgtcgcc gccaatccaa tagtatttgc cgccgcgagg
      721 atcgacacgt tcgatgatat tttcactgta atggcgcttg cccatccggg ttaagcgcca
      781 gcctcgaagc tcggatttcg gcagcggtgg cacgttcaca ttgagaagcg tttcgggcgg
      841 gataccccca gcctgttgga tgcttgagac caagaaggcg gtaaattcag ccgcagcgcg
      901 aaaatcaaaa tcgcgaaatg taaccaacga tacggcaatt gacgggactg ctagcaaggc
      961 tccttcgata gcggctgaaa cggttccaga atagatgatg tcatcaccta gattgggccc
     1021 tttgttgatg cccgaaacga ccaaatgggg ccgaaccggc aagagaccgg tcagtgctag
     1081 tttgacgcag tcgcttgggg ttccgtttac ggccagacgc cgcggcgcaa cctcttgcac
     1141 gcgcagcggc cgatgtaggg tcaatgcatg gctcatggaa ctctgcgcgc gatcgggtgc
     1201 gacagtgaaa atctcgccga cggggcgcag cgcctcttct agcgcgacaa ttccttcaga
     1261 ttgaataccg tcgtcgtttg agatcagaat gatcattgca cctatgatgg caaagaagtt
     1321 cagcagggac aagcatctgt atggcgactc gtcagcttgt gtgacggcaa ttcggcgggt
     1381 ataggtttct agcaaaggag aattttaccg atggcgaatg ccgagatcgt tgcaatcggt
     1441 tccgagttat tgctcgggca gatcgtcgat accaattcgg cctggatggc gcagcggctg
     1501 acagcattgg gcgtaaattt gtattttacc tcggtggtcg gcgacaaccc cggccgtatg
     1561 cgtgaagtga tcgagcgcgc gttggaacga gccgatatcg ttattaccag cggcgggctg
     1621 gggcccactc aagatgacct gacgagagag attgtcgctg aagtgaccgg ccgcaaactc
     1681 atcctcgatg gcggcttgtt agaacaagtc gaggagcact tccgtcgccg tggccggact
     1741 atgacgccca ataaccggcg tcagggatac atgcccgagg gagcgattcc aataaaaaat
     1801 cctaacggca cggcgccatc atttatcgtc gaagatcctc gcggcatcgt tttttcgctt
     1861 cccggagtcc cggtcgaact taaatggttg ttcgagaacg aagtcgaacc ttatctgcga
     1921 agaaaattca acttggctga agtcattcac taccgtgtgc tgaagatcgt tggcatcggc
     1981 gagagcgccg tggacgataa gatcggctat ttgatcgcga actccagcaa tccgacggtc
     2041 ggcgttcttg ccctgcctgg ccaagtcgat gtgcggattg ccgcgaaggc cgccaacaga
     2101 gatcaagcga tgaaccttat cgcaccggtt gaagccgagg tgcgtgcgct gttgggcaat
     2161 gcgatctttg ccgctgatca agagacgatg gaggatgtcg tcggcagatt acttcgggct
     2221 ggtaacaaaa ccgtcgcggt ttatgaagac ctcacctgcg gcagcttatc cgagcgctta
     2281 caggccgcaa gcacggaaag ttttggcgct ggtgttatct gcaacagcga gagtgcaaca
     2341 cgcacgttgt tgactcacgg gcgccatcca gcacagacga atgatttgat ccaagatccg
     2401 gcggccttaa ccgacgagct tgcctggtcc gtgcgccatc aggcggcctg tgatttcggt
     2461 ctggcgcttc atgcggtgac cgatcctgac accaccgttc agaacctagc acgcgggcag
     2521 agctatgttg cccttaccga cggcgagaaa ttcatccgac ggctgagcac ctccgccggt
     2581 cgtggacagt atgaccgcag ccgcatgacg cttaacgctc tcgatctgct acgcaccgcg
     2641 ttgctcgaag gaatgggtta gagtgagcac atcggaagga tcatctgctt cgtattactc
     2701 ttcacatgct caaaacgacc gaagccgacc gggccggtct tagcgatggg agagggcagt
     2761 tcccgagctg tgtcataaaa tttcagccaa taacatctcc aaacgcgctg aaggttcttc
     2821 caggctgtca atcgcctcag cccagaattg cggctgttct atgtccccgc cgattgtttg
     2881 ttgaaccagc ttttctgcgg gctcggtgcc cgccagacgc aagaactcct catatttcgg
     2941 gagaaatccg ctagggtttt ttttgaacat ccggtaaagc gcgcggctaa gcaagtatcc
     3001 gaaagtgtac ggaaagttat agaaggtgat tcccgtgatg tagaaatgca gcttagaggc
     3061 ccagaagtag ggatcctctc caccaacttc aaggacgtcg ccaaaaactt ttcgttgagt
     3121 atcgaccatc agttctttta aacgggagac actcagcggg cccgcggcgc gctcctcata
     3181 gaatgccttt tcaaattcat agcggaccgg aatgtccaaa aggtaaacag ctccatgtcc
     3241 ggtttcgata tccaagatca aagccttttg cgcttgactg atgttgggat cgtccaggat
     3301 gccgttcatc aaaacttcct cgccaaaagt ggatgcggtt tccgccaagg tcatcgggta
     3361 tccgcgacca taagggcgga tgtcacgcat catcgcgcca tgataggcgt gcccggattc
     3421 atgcgcgaga gtgagcacat cgcccaatga ttgattgtag gtcataaaga ttcgcgactc
     3481 cttcgtgagc atcgaagtag tgcagaatcc gccgggacgc ttgccagtgc ggggttccca
     3541 atccacccaa ttcttcccga cgacctgctg ctcgaaaaac ctcccaaggg cgggataagc
     3601 tcgctcgaag gatttcccga ccagggatgt agcgttttcc caggagagct tctcttgacc
     3661 tggtaagtcg agcggcgcgc cgacatcgaa ccaggcaagg cttggccggg ccaacagctt
     3721 ggctttaagc cggagaatct ttcgcggcac ctcgaacctt gagaatagcg cctcgaacat
     3781 agcgtcgagg gtattgcgag tgatagcagc ctggaatagc gctatttcga ggaagtggtc
     3841 gactcgccga tgtcgattga gggtcagtcg tgtgccggca atcgcattca atgcggccgc
     3901 agccatgtct tcgatacttt gccaggccgc attgccgccc tcgaaagcag ccttgcgtat
     3961 gcggcgatca ggatcttcca gcaacgagcg acgctgcgaa atcggcaatc gctggcggtt
     4021 gccgtgggga aatatcatgt cgaattccaa cttcgatgaa atagtatcgt aaagtcggcc
     4081 ccaggcttga atcccgtcga cgccgaggtc agtagcgaga atctccttct ccggcgtcat
     4141 agcgcgccgc gcttcctggc gcatcctgtt taaataattt tgcgcgccgt ccaacgccgg
     4201 ccgcgcaacg aatgccgtga acgtttcctc ctcggtttct ttaatcgctc ggaggaattc
     4261 gattcgcaat ttgcccaact cggctctcat tccgactagc tcggcttctt ctttgaggta
     4321 tgattcgttg cgcgcatccg acgaggcgag gcaaccgatg tacgagccca gatgcgatat
     4381 gcgctggaga atgtcttcat tgcgcagcag tatgcgctcc cagctggcc
//
LOCUS       DASUTG010000170         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1212484, whole genome shotgun sequence.
ACCESSION   DASUTG010000170 DASUTG010000000
VERSION     DASUTG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212484"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..738)
                     /locus_tag="VF089_03810"
     CDS             complement(226..738)
                     /locus_tag="VF089_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017798036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX6173116.1"
                     /translation="MAVKPGWEGRYFEDFEIGDVYRCRLGRTVTEADNIWFTLLTNNT
                     NQIHFNNEYGKRTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDEIKLPNPLFAG
                     DTLYSESEVLEKRESRSKPQWGILKVRTRGVQQEGKVVIDYARSVMVWKKAHAPKKDL
                     FPTIKEDNKS"
     gene            959..2122
                     /locus_tag="VF089_03815"
     CDS             959..2122
                     /locus_tag="VF089_03815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6173117.1"
                     /translation="MMDFNLTPEQQQIREEIKRVCRDFPDSYWREIDRQKEYPEAFVR
                     KLSELGWLAALIPEEFGGTGLGITEASIILEEINHSGGVATACHAQMYTMGTLLRHGN
                     DEQKKRYLPKIATGELRLQAFGVTEPDAGSETTKLQTTAVRKGDKYLINGQKIFISRV
                     LQSDLMLLLARTTPYDELEDKTRGLSVFIVDLRENKEKLEVKPLDLMINHHTNQLFFD
                     NVEVPVENLIGQEGMGFRYIIDGWNAERILVAAEAVGDGRWFIEHAAKYASERIVFGR
                     PIGSNQGIQFPIAQAYAQIEAADLVRYQAATKFDRKEKCGAEANMAKLLASEAAWAAA
                     NACLTTHGGYGFAVEYDVERKFRETRLLTVAPVSNNLVLAYLGQHVLGMPKSY"
     gene            complement(2237..3427)
                     /locus_tag="VF089_03820"
     CDS             complement(2237..3427)
                     /locus_tag="VF089_03820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173118.1"
                     /translation="MKASTVKLLVPLVAITIQSNSASTIIPPYLDYLRIPVGVIGTLI
                     SLGPVLALTSRLPVGMAYNHSRARFLIMLAVLAMGVTNYAYSFATDTITFALVHCLNG
                     FAYGAVTTLYMAFYVDSLAADENRSHAMGYYVGSLAVGYSTGNFFGGLFADHFGYAIT
                     FQGAAFLSLVPCALLWLLRAPAGQGAAKAKEKPKVKLMSREFWRALLEPGLATVVVTA
                     LFLNLLHQMGGVFISLYGLAVGMTLTQIGVLRAAYAGVNAITRPISGHVVNKVGHRGL
                     SYAGIPLQSALLMLIPLFSGFGPILIVYVLSGLMRAIVIVANAVGLVQDVDDTKVQRG
                     IASAVYNSAGDVGNIIGPSIGGIIAHATSIAAVFVYGSLGSTVLFLLVIFAVRRNAGM
                     NYKS"
     gene            3577..>4424
                     /locus_tag="VF089_03825"
     CDS             3577..>4424
                     /locus_tag="VF089_03825"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX6173119.1"
                     /translation="MDLIRSWMFVPGHRQRMIDKALGLNADAIMLDIEDGVAPNEKDS
                     ARKNIAESLGREKVPGTPSRYVRINAIGHARMDADLEAVVRPGLEGLVCPKVDTPDEV
                     RKVDSILTEREPKLGMRNGIVKLLVAIESPRGLLNAPAIAASSARVTGLIFGAEDFGR
                     EIGLPTSREGEARDLIYARSAIVVAAAAAHVQAVDGVWVDLNDTQGLLNFAKQARQLG
                     FSGMSCIHPSQVDAINTTFSPTADEITYARQVLKAFEEANARGDGSIAMGGQLIDRPI
                     VERARRT"
BASE COUNT         1090 a         1188 c         1182 g          964 t
ORIGIN      
        1 ccacagacac ctctttctga cgtcaacagg ttccaaccgt tccagctgtt ccaatcgttt
       61 cagccgttca aatttcaatc agccaccgtc tttcggcttt catggcttga ttgaattttg
      121 ccacatccga agcttcgcag cttccgaacc attggaaccg ttggaacgat tggaacggtt
      181 tggaaccaag taattttttt gaaatacagg cgaacttttg caactctacg atttattatc
      241 ttccttaatg gtgggaaaga gatctttttt cggcgcatgc gcttttttcc agaccatgac
      301 gctgcgggcg taatcgatga cgactttgcc ttcttgctga acgccgcggg tacggacttt
      361 tagaatgccc cactgcggct tcgacctcga ctcgcgcttc tccagcactt cggattcgga
      421 atagagcgta tctccggcaa acagcgggtt cggcaatttg atctcatccc aacccagagc
      481 aaaaccgttt tcgctcacgt cagcaacccc cattccggca acaatagcca aggtcaacgc
      541 gctgttaatc agacattttc cgaactcggt ccgcttgccg tattcattgt taaaatgaat
      601 ctgattggtg ttgttggtga gcagagtaaa ccagatgttg tcggcctcag tgaccgttcg
      661 tcctaaccgg caacgataga catcgccgat ctcaaaatct tcaaagtacc gaccttccca
      721 tcccggcttg acggccataa ccttcctcct cgattggcaa caaaaaccat ttcgctatag
      781 tctagcgagt gctgaagtaa ttctcttgcg ggctgctcaa aaagatctca gaggggaggc
      841 gcgcgaagta tcaacgagct gaggcgtact ttagcagtac gtcgaagcga ggcgatcgag
      901 cgcaacgaag catatgagcc tttttcagca gcctgctacg ggctaattat aggaggaaat
      961 gatggatttc aacctaacac cagagcagca acaaatccga gaagaaatca aaagggtgtg
     1021 cagagatttt ccggattctt actggcgcga gattgacagg caaaaagaat atcccgaagc
     1081 attcgtgcgc aaactctccg agctgggctg gctcgccgcg ttaattccgg aagaatttgg
     1141 cgggaccggt ctcggcatca ccgaagcgag catcattctg gaagagatca atcactccgg
     1201 cggcgtcgcc actgcatgtc atgctcagat gtacacgatg ggcaccctgc tccgccatgg
     1261 caatgacgag cagaaaaaaa ggtatctgcc gaagatcgct actggcgagt tgcgcctgca
     1321 ggcgttcggc gtcactgaac cggacgccgg atctgaaacc acaaagcttc agaccaccgc
     1381 tgttcgtaaa ggcgacaagt atctcatcaa tggccagaag atcttcatct cccgggtgct
     1441 ccaatccgat ctgatgctgc tgttggcgcg gacgacgcct tacgatgagc tggaagacaa
     1501 aacccgtggc ctttccgtgt tcatcgtcga ccttcgagaa aataaagaga aactagaagt
     1561 taagccgctg gatttgatga ttaaccatca tacgaaccaa ctattcttcg ataatgttga
     1621 agtgccggtg gagaatctca ttggccagga gggcatgggt tttcgctaca tcatcgatgg
     1681 ctggaatgcg gaaagaatct tagtggcggc cgaagccgtc ggcgatgggc gctggttcat
     1741 cgaacatgcg gccaagtacg cgtctgaacg gattgtcttc ggccggccta tcggttcgaa
     1801 tcagggcatc caattcccca tcgcccaggc gtatgcgcaa atcgaagcgg cggatctcgt
     1861 ccgctatcaa gctgcgacga aattcgatcg caaggagaaa tgcggcgcag aagccaacat
     1921 ggccaagctt cttgcctccg aagctgcgtg ggcggcagcc aacgcttgcc tgacgactca
     1981 cggcggctac ggttttgccg tggagtatga cgtcgaaaga aaattccgag agacgcggct
     2041 gttgaccgtc gcgccggtga gcaacaactt ggtgcttgcg tatctcggac agcacgtcct
     2101 gggtatgccg aagtcctatt aaagacaacg atgaaagctg aaaacggaaa gcggaaataa
     2161 cgatcgagcc gggatctttt tcagatttca gttttcataa ttcatccttg cctttccagc
     2221 ggcttcgttt cagctttcag ctcttataat tcatccctgc atttcgccgc accgcgaaaa
     2281 tcaccagcag gaatagaacc gtcgagccca gtgatccata aacaaacacg gccgcgatgc
     2341 tggttgcgtg ggcaatgatt ccgccgatcg acggaccgat aatattgccg acgtctccag
     2401 ccgagttata aaccgctgaa gcgatgccgc gctgcacctt ggtatcatcg acgtcttgca
     2461 ccaatccaac cgcgttggcg acgatgacga tggcgcgcat caggccggac aaaacgtaga
     2521 cgatcaagat tggcccgaac ccgctgaata gtgggatcaa catcagcagc gcggattgga
     2581 gcggaatacc cgcgtacgat aaaccgcgat ggcctacttt attaaccaca tgaccgctga
     2641 ttgggcgcgt gattgcattg acgccggcat aagccgcgcg gaggacgccg atctgggtca
     2701 gagtcatgcc gacggccaat ccgtacaggg atataaagac gccgcccatc tggtgcagca
     2761 aattgagaaa caatgccgtt actacgacgg tggcaagtcc cggctcgagc agtgctcgcc
     2821 aaaactctct ggacattaac tttactttgg gcttctcttt ggctttggct gctccctgac
     2881 ctgcgggcgc tcgcaacagc cacaacagcg cgcatggcac cagcgacaaa aaggcggctc
     2941 cctgaaacgt aattgcatag ccgaaatgat cggcaaagag cccaccgaaa aagtttcccg
     3001 tcgaatagcc caccgcaaga ctgcccacgt agtaccccat ggcgtgacta cggttctcgt
     3061 cagctgccaa ggaatcgacg taaaacgcca tgtagagtgt cgtcaccgcg ccgtaggcaa
     3121 acccgttgag gcagtgaacc agtgcgaagg taatcgtgtc agtggcaaag ctgtaggcgt
     3181 agttcgtcac tcccattgcg agaaccgcca gcatgatgag aaagcgggcg cgactgtggt
     3241 tgtacgccat gcccaccggc agtcgcgatg tcagggcgag aaccggccca agtgaaatca
     3301 aggtgccgat cacgccgaca ggaatgcgca gataatcaag atagggcgga atgatggtcg
     3361 atgcggaatt gctttggatt gttatcgcga ccagtggcac caggagctta accgtgcttg
     3421 ccttcatcgc cgatgaattt gcaatcttga gtattgagaa ttcatctttc ttttattctc
     3481 taaaaggatg agggttgcaa acaagcgcgc cgaatacggc caagatagac atttcaaagc
     3541 ccttcaggta tttgaatttc gataggagaa cgtccaatgg atctgattcg ctcatggatg
     3601 tttgtgccag ggcacaggca acggatgatc gacaaagcgc taggcctgaa tgccgatgcc
     3661 atcatgctcg acatcgaaga tggtgtggcg cccaacgaaa aagatagcgc gcgcaaaaac
     3721 atcgccgaat cgctgggccg tgaaaaggtg cccggcactc cgtcacgtta tgtgcggatc
     3781 aatgccattg ggcacgctcg catggacgcc gatttggaag ctgtcgtgcg tccgggcctt
     3841 gaaggattgg tttgccccaa ggtggatacg ccagatgagg tccgcaaagt cgattcaatc
     3901 ctgacggaac gtgagccaaa gttaggaatg cgaaacggca tagtcaaatt gctggtcgcc
     3961 atcgagagtc cgcgaggttt attgaacgca ccggcaattg ccgccagctc cgcacgcgtt
     4021 actgggctca ttttcggggc cgaggatttt ggccgggaaa taggattgcc gacttcgcgc
     4081 gagggcgaag cccgcgacct tatctatgcc cgttccgcca ttgtcgttgc cgctgcggcg
     4141 gctcacgtgc aggcggtcga cggcgtctgg gttgacctaa acgacaccca gggtcttttg
     4201 aactttgcca aacaagcccg ccagctgggc ttttccggca tgtcctgtat tcacccatcg
     4261 caggttgatg ctatcaacac gacattcagt ccaacggcgg atgaaataac ttatgcaagg
     4321 caagtactga aagccttcga agaagccaat gcgcgcggcg acggctccat tgccatgggc
     4381 ggacagctca tcgatcggcc catcgttgaa cgagcgcgcc ggac
//
LOCUS       DASUTG010000171         4411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_471500, whole genome shotgun sequence.
ACCESSION   DASUTG010000171 DASUTG010000000
VERSION     DASUTG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471500"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VF089_03830"
     CDS             <1..528
                     /locus_tag="VF089_03830"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX6173120.1"
                     /translation="SNCVLPSPSATIVLDMIIVITGVTGSGKTTIGRRLATELQWKFF
                     EGDDFHPPANIEKLKRGEPLDDQDRLPWLTAIRNTIRAAIGRGENAVIACSALKESYR
                     RMIQISPQVVLVHLQADPELIEKRLKQRIGHFMNRNLVRSQFETLEEPQAALQIDASL
                     TPEQLVASIRSRLSL"
     gene            572..1069
                     /locus_tag="VF089_03835"
     CDS             572..1069
                     /locus_tag="VF089_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6173121.1"
                     /translation="MAQTIGTPLHFIPPGSIARVVNRFYGSLTRLGLSMPYSFLLSVP
                     GRRTGKTRSVPVNLLQHDGKLFLGATRGHTQWARNARASRKIFLARGRLRLGFSLREI
                     PDNEKAAVLQSYLTRFNWMAWRFFPVRAGSPLGAFEPIAARYPVFELIRIRRESSTES
                     PDSNP"
     gene            1129..1485
                     /locus_tag="VF089_03840"
     CDS             1129..1485
                     /locus_tag="VF089_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6173122.1"
                     /translation="MAALVLLLVRHYQMISFSGAVICFVAWVVKDWILYPWLRSAYEI
                     SNRTGSKTLIGHKGVAESHLAPEGLIRVRGELWRAVAIPRDLTIQTGIVVEIIDADGM
                     TVFVRPLRDRAANDST"
     gene            1623..2726
                     /locus_tag="VF089_03845"
     CDS             1623..2726
                     /locus_tag="VF089_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6173123.1"
                     /translation="MLILSNEEIESFLSIDKCINALEKAYASWDEGKAINRPRTDLVM
                     PSSNGTGVYAFKSMEAGLYDPPIVAMRINSDIIRWQQQGSRVIKTKVPAAPGDKYVGL
                     VMLFSTLTGEPLAIFPDGVVQRMRVASSSALAARYLARHDASTLAIFGSGWQAGSHLP
                     AMCAVRNIKNVNVFSPTKSNREAFVREMAQKVSAEVRPVDSAEEAVRDADIIAATTNS
                     LTRVVNPEWVRPGIHLTCVRVPELGDETIQKIDRLVIHSRQHAPENYIAGFGDQGIES
                     HDAIDIIRKGPAQAREAKVEHPFWLASPELRDLVIGRAQGRANAQESTCFLNNIGIGL
                     QFAAVGAAVLAEAKTKGAGHEIPTDWFLETVHP"
     gene            2859..3842
                     /locus_tag="VF089_03850"
     CDS             2859..3842
                     /locus_tag="VF089_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173124.1"
                     /translation="MIGFLRIAVVILLTGFVVAPAVAQDKLRFYLGASSKTLGYSPLW
                     VAAKKGFFEQQGLDVQLLLLRGTPMTVQALAGASLNVGSGSPEAFIEASERGLDLVII
                     GGVINGLTHYIVGGKNYRTYQDLRGAALGASSLTSGTVTAFKQALKIKGLEYPRDYKI
                     LVVAGGSSANLAALQAGQIAATTVAIPLNYLAEEAGFNVIGRLIDAVPDFQLTVIAAK
                     RSWAEKNRSSMVRFMKGMAQANRWLYENKEPAIDFLTHEMQLKPIHAARGLEYYTQNR
                     IWHPEGNLNLEGMKYNLRIYAEQTGAKGAPDYGKYIDQSYLYDALKELKKK"
     gene            complement(3916..4170)
                     /locus_tag="VF089_03855"
     CDS             complement(3916..4170)
                     /locus_tag="VF089_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173125.1"
                     /translation="MRAARKDVRESRRELQGDYKELRKDKAELRRDIRNGASKEEIRR
                     GRQEIRDDYKEIARDRRDLRQDQAELRDARGELKDALHKR"
     gene            4207..>4411
                     /locus_tag="VF089_03860"
     CDS             4207..>4411
                     /locus_tag="VF089_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173126.1"
                     /translation="MTPIALVLSAMSVPPVTRIIEARVSRLCATMSAMAIKSTIVTSP
                     VLSSEFSVVTVAPAMFVSTIVFGV"
BASE COUNT          950 a         1163 c         1247 g         1051 t
ORIGIN      
        1 tctaattgtg tccttccatc tccaagtgct acgattgttc tcgacatgat aattgtgatc
       61 acgggtgtaa ccggctccgg caaaactacc atcggcaggc ggctggccac tgagctgcag
      121 tggaaatttt ttgagggcga cgattttcat ccaccagcta acatagaaaa actaaagcgc
      181 ggcgagccgc tcgatgatca ggatcgtctc ccttggctca cggcaatcag aaataccata
      241 cgtgcggcga tcggacgcgg tgaaaatgcg gtcatagcct gttccgccct taaggagtcc
      301 taccggcgaa tgattcaaat cagcccgcaa gttgttctgg tgcatctaca ggcggatccc
      361 gaactgatag aaaagcggct gaagcagcgc atcgggcact tcatgaaccg caatttggtt
      421 cgaagccaat tcgagacgct cgaggagccc caggctgcgc tgcagatcga cgccagccta
      481 acccctgaac aactcgtcgc cagcattaga tctcgtctct cgctgtaatc gaggcagccg
      541 ctctcagtgg ttgcgtgtgg catcggtaga gatggctcaa acgataggaa ctccgctgca
      601 tttcattcct cccggatcga tcgcccgcgt ggtgaatcgg ttctacggct cactgaccag
      661 gctcggattg agcatgcctt acagtttcct attgtcggta cccggccgaa gaacgggcaa
      721 gacgcgctcg gtaccagtga atcttttgca gcacgatggc aagttgtttc tgggggctac
      781 tcgcgggcac acgcagtggg cgcgcaacgc gcgtgcgagt cgaaagatat tcttagcgcg
      841 agggaggcta cgtctggggt tttccttgcg agaaatcccg gacaacgaga aggctgccgt
      901 gttgcaatct tatttaactc gattcaattg gatggcctgg cgcttttttc ccgtacgcgc
      961 cggttcgccg ctgggagctt tcgaaccaat cgccgcgcgt tatccggtgt tcgaactaat
     1021 tagaattcgc cgggaaagct ctactgaatc gccggattca aacccatagt cactggttcg
     1081 atgcctacct ggttcagata tttactattc caaattcccg gctggtgtat ggcggcgctc
     1141 gtgttgctgc tcgttcgaca ttaccaaatg atttcgtttt caggcgcggt gatttgtttc
     1201 gttgcctggg tggtgaagga ttggattctc tatccatggc tcagaagcgc ctatgagatt
     1261 tctaatcgga cagggtcgaa gacgctgata gggcataaag gcgtggctga gagtcatctc
     1321 gcaccggaag gcttgattcg ggttcgcggc gagctctggc gggcggttgc catcccgcgc
     1381 gatctgacaa ttcaaacggg gattgtcgtg gaaataatcg atgccgacgg tatgacggtt
     1441 tttgttcggc cgctcagaga ccgagccgcc aatgattcca cgtagaagga ttcgttaatg
     1501 tttgcctgag agcactttcg tctcatacgc cggcgttcgc ctgactcaga cgcagcaatc
     1561 tgcgttgcac ctccaggtat gtcttcttga cctcttttca tcccgttgat atgccagcaa
     1621 gcatgctgat tttaagcaac gaagaaatcg aatcctttct gtctatcgat aagtgcatca
     1681 acgcgctgga gaaagcctat gcaagttggg atgaggggaa ggcaatcaac cggccgcgca
     1741 ccgacctggt catgccctcg tcaaacggga ccggtgttta cgcttttaaa tcgatggaag
     1801 cggggcttta cgatccgccg atcgtggcga tgcgcatcaa ttccgacatt attcgctggc
     1861 agcagcaggg cagtcgggtc atcaaaacca aagtgcccgc ggctcccggg gacaaatatg
     1921 tcgggctggt gatgttgttc tcgaccttaa ccggcgagcc tctcgcgatc tttcccgatg
     1981 gcgtggttca gagaatgcgt gtggcatcga gcagcgcttt ggccgcgcgg tatctggcgc
     2041 gccacgatgc gagcacccta gcgatatttg gatccggctg gcaggcgggc agccatctgc
     2101 cggcgatgtg cgcggtgcga aatattaaaa atgttaatgt ctttagtccg accaaatcca
     2161 accgcgaagc attcgtaaga gaaatggctc agaaggtcag cgcggaggtg cgcccggtgg
     2221 acagtgccga ggaggcggtt cgtgatgccg atattattgc cgcgacgacc aattcactga
     2281 cccgtgtggt caatcctgaa tgggttaggc cgggcattca cctgacctgt gtgcgggttc
     2341 ccgaactggg agacgaaacg attcagaaga tagatcgcct ggtgattcat tcgcgccagc
     2401 acgcgccgga aaattacatt gccggatttg gcgaccaggg gattgagtct cacgatgcca
     2461 tcgacatcat tcgcaaagga ccggcgcaag cgcgcgaagc aaaggtcgag catccattct
     2521 ggttagcctc gccggaactg agagatcttg tgattggacg agctcaaggc cgggcaaatg
     2581 cgcaggagtc cacttgtttt ctcaacaata tcggcatcgg cctgcagttt gccgccgtcg
     2641 gcgccgcggt tttggccgag gcaaagacta agggcgccgg acacgagatc cccactgact
     2701 ggtttttaga aacggtccat ccgtaacaag ccgaagtgta acgatcgacg ctgcttgaac
     2761 tcgataaggt gtgatggcgg atgagcgcgg cttcctccaa ctgcttgaat gatttgaacc
     2821 gcttgaacgt cttgaatgat atttagaagg gtaaaagcat gatcggcttt cttagaatcg
     2881 ctgttgttat tctgttaacc ggcttcgttg tcgctccggc agtggcgcag gacaagctgc
     2941 gtttttatct tggcgcatcg agtaaaactc ttggttacag cccgctttgg gtggcagcga
     3001 aaaaaggctt cttcgagcaa caaggtttgg acgtgcagct tctccttttg cgcggcacgc
     3061 cgatgaccgt tcaagcgttg gcgggggctt ccctcaacgt cggttccggc agcccggaag
     3121 cgtttatcga agcctccgag cgcggcttgg atttggtgat cattggcggc gttatcaacg
     3181 ggctgacgca ttacattgtc ggaggtaaaa attaccgaac ctaccaggat ttgcgcggcg
     3241 ccgctctagg cgcttcttcg ttgaccagtg ggacggtgac ggcattcaag caggccctca
     3301 agatcaaagg gctcgagtat ccgcgagact ataagatcct tgtggtggcc ggcggctctt
     3361 ctgccaatct tgcggcacta caagcaggcc aaatcgccgc cactaccgta gcaatcccgc
     3421 ttaactatct ggcagaggaa gcaggcttca atgtgatcgg cagactgatc gatgctgtgc
     3481 ccgatttcca attgaccgta atcgcggcca agcggtcctg ggcggaaaag aaccgctcgt
     3541 caatggtgcg cttcatgaag ggcatggcac aagcgaatcg ctggctctat gaaaacaaag
     3601 agccggccat cgatttcctc acacacgaaa tgcaactgaa gccaatccac gctgcgcgcg
     3661 ggttggaata ctacacacag aaccgtatct ggcatccgga ggggaatctt aatctggaag
     3721 gcatgaaata caatctgcgc atctatgccg agcagacggg agcgaaggga gcacctgatt
     3781 acggcaagta catcgaccag agttatctct atgatgcatt aaaagagttg aagaagaaat
     3841 aagctgagga gcttactttg cagatggaaa gcctgaccgc ctgcgcttaa cagacggtca
     3901 ggtgctaaga ttggcttatc gtttgtgcag agcgtctttt aattctccgc gtgcgtctcg
     3961 cagttcagct tgatcctgcc gaagatctct tctgtccctg gcgatttcct tgtagtcatc
     4021 gcgaatctct tgtcgacccc gacggatttc ctctttgctg gcgccgttgc gaatatcgcg
     4081 gcgaagctcg gctttatcct ttctcagctc cttgtagtcg ccttgtaatt cgcggcggct
     4141 ttcccgcacg tctttgcggg cggcgcgcac gttgctgaat ttctctctaa ccgccggttt
     4201 gttgtgatga ccccgattgc cctggtactt tccgcgatgt ccgtcccgcc ggtaacgcga
     4261 atcatcgaag cgcgcgtgtc gcgcctctgc gcgacgatga gtgccatggc gattaaatcg
     4321 acgatcgtta cttctccggt gttgtccagc gaattttcgg tggtcaccgt cgcgccggct
     4381 atgtttgtat cgactatcgt ctttggcgtc c
//
LOCUS       DASUTG010000172         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_175530, whole genome shotgun sequence.
ACCESSION   DASUTG010000172 DASUTG010000000
VERSION     DASUTG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175530"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1312
                     /locus_tag="VF089_03865"
     CDS             119..1312
                     /locus_tag="VF089_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6173127.1"
                     /translation="MPIGTPFHSRTSALCTSQNWRTWSGYFAASSYDVVHDYEYHAIR
                     NTAGLIDISPLFKYDVRGKDALKLVNRVITRDVAKSAVGQAVYGCLCDGEGKVIQDGT
                     VFRLGEDQFRFHLAEPALRWLSLNAGGMSVSIDEVSERIAALALQGPNAVKILTQIVD
                     AELGKLKFFRLMSADFAGIPVTISRTGYTGDLGYEIWLPATHAERVWDVLTDRGKPYG
                     LKAAGMLALDVARLEAGFILLEVDYVGADKALIASQKYSPFEIGLGWTVDLGKDFFVG
                     SKALRHSDGKDVPRQITGLEVDWRHYEELYQKVGLPPHVPAAAWRGGIPLYKDDLQVG
                     QATTGAWSPSVKKYIALATIKSEHAIPGTTLQMETTVEYERKKVRVTTVKPPFFDPPR
                     KRMLF"
     gene            complement(1404..2165)
                     /gene="hisF"
                     /locus_tag="VF089_03870"
     CDS             complement(1404..2165)
                     /gene="hisF"
                     /locus_tag="VF089_03870"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX6173128.1"
                     /translation="MLAKRIIPCLDVNEGRVVKGVKFLELRDAGDPVEIARQYDKEGA
                     DELCFLDITASHQQRRIILDVVARTAEQVFMPLTVGGGINTLEDIRNLLQAGADKVSI
                     NTGAVNRPDFVREAAEVFGSQCIVVAVDAKRVNDHWEVFTHGGRNSTGLDAGEWAERM
                     EKFGAGEILLTSMDRDGTKAGYDLALTRAVSDRVTIPVIASGGVGNLDHIYQGFAEGN
                     ASAALAASIFHYREYSVAQCKKYLHERGIPVRLQK"
     gene            2534..>2914
                     /locus_tag="VF089_03875"
     CDS             2534..>2914
                     /locus_tag="VF089_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173129.1"
                     /translation="MKNRFIGLGMLSAFLLATPVLAQNVDDKIQALEQELMQLKEQQI
                     ELKKEATEAAAAMPSFSYRPGNGLNVEAADKAWSFRASLESHFRMNFLSGRDEIGRTN
                     GEIEGRRFRPSFFYCLNNCLWELEA"
BASE COUNT          690 a          747 c          798 g          679 t
ORIGIN      
        1 tcgaggattt ggaactaggc gaagtaacat cgattcaaag atagagtaat caggcgaaaa
       61 gctgcgcgcg atcgtatcga ctattctgtc tccgccgaaa tcggtctcct tacatttcat
      121 gcccatcggg actcctttcc actcacggac ctccgcgctc tgcaccagtc agaattggcg
      181 cacctggtcg ggatacttcg ccgccagttc gtacgatgtg gttcacgatt acgaatatca
      241 cgcgatacga aacaccgctg gcctcatcga catctcaccg ctgttcaaat acgacgtccg
      301 gggcaaagat gcattgaagc tggtcaatcg ggtgatcacg cgagacgtcg ccaaatccgc
      361 cgtggggcag gccgtttacg gttgcttgtg tgacggcgaa ggaaaggtca ttcaggacgg
      421 tacggtgttt cgcttgggtg aggatcagtt tcgttttcac ttggccgaac ccgcactgcg
      481 ttggctgagc ctaaacgccg gagggatgtc ggtttcaatc gacgaagtat cggagcggat
      541 cgcggcgctt gcgctgcagg gacccaatgc ggtgaaaatt ctaacccaga tcgtcgatgc
      601 cgagttgggt aagctaaagt tctttcgttt gatgtcagcc gatttcgcgg ggatccctgt
      661 taccatttcc cgcacgggtt ataccggtga tctcggatat gaaatatggc tgcctgccac
      721 tcatgcagag cgtgtatggg acgtcctcac cgacagaggc aagccatacg gtctaaaggc
      781 agcgggaatg cttgcgcttg atgttgcccg gctggaagcg ggatttattc tgcttgaggt
      841 cgactatgtc ggtgcggata aagcccttat cgcgtcgcag aaatactccc cgttcgagat
      901 tggtttgggc tggacggtgg atcttggcaa ggattttttt gtcgggagca aggcgctgcg
      961 tcattcggat ggcaaggatg tgccgcgtca gatcaccgga ctagaggtcg attggcgcca
     1021 ttacgaagaa ctctatcaga aggtgggact tccgccgcat gtcccggcag cggcatggag
     1081 aggtggaatt ccgctttaca aggacgatct tcaggttgga caagcgacga caggcgcatg
     1141 gtcgccgagt gtgaaaaaat acatcgcgct ggcaaccatc aagagcgagc acgcaattcc
     1201 cggaaccacg ctccagatgg aaacgacggt ggagtacgag cgaaaaaagg tacgagtcac
     1261 tacagttaaa ccgccatttt ttgatccgcc tagaaaacgc atgcttttct aataagggca
     1321 acttcggagc gctgttccag gcggaccctc ctcagttcca acctcactga tctgactagc
     1381 agtttagccg gattcgaaca aagttacttc tgtaagcgta cgggaatgcc gcgctcgtgc
     1441 agatactttt tacattgtgc cactgagtat tcccggtaat gaaagataga ggcggctagc
     1501 gctgcgctgg cgtttccttc agcaaatccc tggtagatgt gatccaagtt tcctacgcca
     1561 cccgaggcga tcacgggtat tgtaacccgg tctgaaaccg cgcgggtcaa agcaagatcg
     1621 taacctgcct tggtgccgtc gcggtccatg ctggtaagaa gaatctctcc tgccccgaat
     1681 ttttccattc gctcggccca ctcaccggca tccaatcctg tggagttcct tccgccgtga
     1741 gtgaagactt cccaatgatc gttaacacgc ttagcatcga cggcaacaac gatgcactgg
     1801 ctgccgaata cttccgccgc ctctcggaca aaatctggac gattgaccgc gcccgtgttg
     1861 atcgaaacct tgtcggcgcc ggcctgtagc aaattgcgga tatcttccaa ggtattgatt
     1921 ccaccgccga ccgtgagagg catgaaaacc tgttcggcag tgcgcgccac gacatccagg
     1981 atgattcttc gctgttgatg cgatgccgtt atatcgagga aacaaagttc atcagcgcct
     2041 tctttgtcgt actgacgggc aatctcaacc gggtcgccgg catcgcgaag ttccaaaaac
     2101 ttcacacctt tgacaactct tccttcgttc acatccagac agggaatgat tcgtttagct
     2161 aacatggagc gaagtctcac caggcgttga tgaaatctgg aacgtgcgtg gctagcccgc
     2221 ctacggcgca acacctgtgc tatctaggcg gtttagacgg tacctagaga tagtcgtctt
     2281 tacaagaagg cacgagttga atgtctgttc tgcccgctcg aggccaggcc agaaacagct
     2341 gtagcccgcg attaagactt tggacctgga tcgcttttgc aagtctccgt taagtccatc
     2401 gctcaactat aatcaactgt cggccatgct cactaaagta caactttagg cttgacactg
     2461 ttttgggagt aggctaaaag tcgcgctaac agtaccaaag atgtacaaaa ttcgaaggag
     2521 gcaaggaggc aaaatgaaga ataggtttat cggattagga atgctgtctg cgttcctttt
     2581 ggctactccg gttctagcgc agaacgtgga tgataaaatt caggcgctcg agcaggaact
     2641 gatgcagttg aaagagcagc agatcgagtt gaagaaggag gcaactgagg cggcagcagc
     2701 gatgcccagc tttagctacc gcccgggtaa cggtcttaat gttgaagcgg ccgataaagc
     2761 ctggtccttt cgtgcaagtt tggaatcgca tttccgcatg aattttctga gcggcagaga
     2821 tgagataggc cgtaccaacg gagaaatcga aggccggcgt ttcagacctt cgttcttcta
     2881 ctgcctgaac aactgtctgt gggaactcga ggca
//
LOCUS       DASUTG010000173         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_323744, whole genome shotgun sequence.
ACCESSION   DASUTG010000173 DASUTG010000000
VERSION     DASUTG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323744"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF089_03880"
     CDS             <1..201
                     /locus_tag="VF089_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173130.1"
                     /translation="DKRRLSLSTQFHSSMRFSWSCPETPKPIESAKQQPANPENLIVI
                     TEADTCRKYVLPKLYAAGWNDD"
     gene            313..495
                     /locus_tag="VF089_03885"
     CDS             313..495
                     /locus_tag="VF089_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173131.1"
                     /translation="MLAVVEAKPSYKLAGDGLQQAKEYAEVLGIKFGYSTNGHSIVEF
                     DYSTGKERELETFPSP"
     gene            complement(653..813)
                     /locus_tag="VF089_03890"
                     /pseudo
     CDS             complement(653..813)
                     /locus_tag="VF089_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012518683.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            1404..2090
                     /locus_tag="VF089_03895"
     CDS             1404..2090
                     /locus_tag="VF089_03895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6173132.1"
                     /translation="MSRARLYVTAVLCSSLLALQLSGCSATPRQQDDILYLAVGASDA
                     LGIGASPLRNGYVYRIRDELEQQTRRNVRLLNLAIPNGTTRELRQALQMALVKELKSD
                     LVTVWTGANDLIRGQAPEDFENDLSDLLKQLRENSPFIVIADIPDLTKSPRFRTRPSP
                     TVTPDRLAAFNRVIEKQASAINAPIVRLSREQITDDLVNEIDGFHPNDRGHRQIAQLF
                     LKVILPRFAV"
     gene            2712..>3766
                     /locus_tag="VF089_03900"
     CDS             2712..>3766
                     /locus_tag="VF089_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin oxidase"
                     /protein_id="HEX6173133.1"
                     /translation="MITRRAFLKYTGGTALTLFAFNKFGLPKAIAQIPGGTLDPDAVA
                     KFVTPLLIPPVMPTAGRIVLQGGKNADYYEISMKQISQQILPTGLPATTVWGYGAVAS
                     DSKRGLLLHNAPSLTIEARWNTPVRVKWINDLKDAAGNYLPHLLTVDPTLHWANPPGG
                     LTGRDTRPTFTSTPGPYTGPVPIVTHVHGAVGVGDESDGYAEAWYLPAAGNIPAGYAT
                     EGTWYNFFKGKAATAHEVTWGSGFAIFQYPNANRASTVWYHDHVLGMTRLNVYAGPAG
                     FYIIRGGPDGDDAVLDSRSGRPAVLPGPAPKENDQFPPNKTYYEIPMAIQDRSFNTDG
                     SLFYPNTREFFDEIVGD"
BASE COUNT          922 a         1044 c          978 g          822 t
ORIGIN      
        1 gacaaaagac gtctgtccct ttcaactcag tttcatagtt ctatgagatt tagttggtct
       61 tgcccagaga cacccaagcc aatagaatcc gccaaacagc aaccagcgaa tcctgaaaac
      121 ttgattgtga tcactgaagc cgatacctgc cgaaaatacg tcctcccgaa actctacgcc
      181 gcgggctgga acgacgacta aataagcgaa cataaaagct tcaccgatgg acgcatcatc
      241 gtcataggaa gcaagccgcg tcggcgtccg gcgaaacggg ccgactactt gctgcggtac
      301 gcgcgggatt tcatgctcgc ggtcgtcgaa gccaagccat cgtacaaatt ggccggagac
      361 ggactgcaac aagcaaaaga gtatgctgaa gtccttggga ttaaattcgg ctattcgaca
      421 aacgggcaca gcattgtcga attcgactat tcgaccggga aagaacggga gttggaaacc
      481 tttccttctc cataagaatt atgggaccgg tatcgaagcg ccgacaactt caccgatgaa
      541 gaaaccgcgg agaaactgct cacgccttcc aatacagttg tcggcaaagg ccaccggcac
      601 cggaagagtc tggaacatgc gctggaaccg cgccggcgag caccgccgcc gaaaaattgc
      661 tttggtcttt ggcgctcctg cgatgacact catcgacgat gatcaagtca aaaaattctt
      721 tcgcatattc ttatagagcc ctggacggtt ctcgtttacg tcgccgttct cgattttgca
      781 gcgagcatcg ccaaacgtgg tgaacatctt gtctacagct tgcgccaggg catcgaggat
      841 gcattccttg ccctgtcgaa cagatgattg caaggtcagg gggaaatcgg cagggacagg
      901 ctttcacttc tagaaactct cttcgccctg aagatctcca gaagtgagta taaaagccgc
      961 gacctttgca gtcgtgagag tggcgcgttc gatattgcga tacctaatgc ggtcatcttt
     1021 ggtgagaaca accctgccgt gctccccgac tacagagagc cattcttcgt ctcgggcatc
     1081 gggcgaaaag taatcatcgt gtaggtggac tgttgctcct ggattgcata gagcgaaacg
     1141 cagcggaaat aacgttcgtt gcacttggtg tcgaaatcca ggttcatgca gtggaatacc
     1201 tatgatcaaa tgaatgctgc gggtgtagga gtagaggcct acccggtcgt gtttaacctc
     1261 gagtcatccg gcccgacaga gcctgccacg cgatgcacta aacaaacgga ttgtgtccgc
     1321 catgccttgg ttaaacggat tgcgcggcgg ctcaaaagtg tcatatcctt gagtcgagaa
     1381 gtgattcaat ggggaggtat ccattgtcgc gagcaaggct ttatgtgacg gcggtactct
     1441 gttcttctct actggctctc cagctttccg gctgttctgc gactccacgc caacaagatg
     1501 atattcttta ccttgccgta ggcgctagcg atgcgctggg aattggagca tcgcctctac
     1561 gaaacggtta tgtgtatcgc atcagagatg agttggagca acagactcga agaaatgtaa
     1621 gactgctgaa tctggctatc cccaacggga ccacgcggga gcttaggcaa gcgttacaga
     1681 tggctctcgt taaggagcta aagagcgatc tggtcaccgt ctggaccgga gcgaacgacc
     1741 tcatccgagg tcaagcaccc gaggattttg aaaatgatct aagcgatctc cttaaacagc
     1801 ttcgggagaa ttccccgttt atcgtcattg cagatattcc tgatctgact aaaagccccc
     1861 ggtttagaac tcggcccagt ccgactgtga cacccgatcg gctcgccgca ttcaatcgag
     1921 taattgagaa gcaagccagc gcaatcaatg caccgatcgt caggctttcc cgagaacaga
     1981 tcacggatga tcttgtaaac gaaatagatg gttttcaccc taacgataga gggcaccggc
     2041 agatcgcaca gctatttctg aaagtcatcc tgccgcgatt tgcggtttaa gctcggccgt
     2101 cagcggataa attgtgcatc tgcttcgatc tttcaatttc gcgagttgta ccgaattcct
     2161 gctcggccgg ccggttcgct tgtgaggcac atcctacgtc gtcagttaca tacttacgcc
     2221 aactcaattt agacatccaa gtcactggat ttcgccaaac agcagtgagc agtcaccatc
     2281 cttatcgata gtaatggtag tgatcgtcgc ctgatctact gcacaggcaa tggacaggtt
     2341 tccagccgat ctttagtcaa cccgcgcggc gtctcaattc tcacggttaa aatcaatctt
     2401 ctttggtgtt ctcatatttg acaatttacg gtcgaaaata cgtaaaaaat ctaaaaactg
     2461 ccttcatggt acaagccttg ctctaaaggc cagacagaac tcaggaggaa aaaaaggata
     2521 ccgcagaagg cagtttctta acttagcgcg atatcaggcg ctggcgtatt tcgcatcgat
     2581 gagcggttaa acatcttcgc ctaaccattg taggcgagcg ggacaatgct aaagcgacat
     2641 gcctcaactt ggcccgtcag ccaatggcag tcgacggtac gcgcaatttg gaggaatgga
     2701 ggttcgagat catgattaca cgacgcgcgt tcctgaaata caccggaggc acggcactga
     2761 cactgttcgc tttcaacaag ttcgggcttc ccaaggcgat cgcacagatt ccaggcggca
     2821 cgctggatcc ggacgctgta gcgaaattcg tgacgccgct gctcatccca cccgtcatgc
     2881 caacggccgg aaggatcgtc ctgcagggag gcaagaacgc cgactattac gagatctcaa
     2941 tgaagcagat ttctcagcaa attctcccca ccggcctgcc cgccaccact gtctggggct
     3001 atggcgccgt ggcgtccgac agcaaaagag ggctgttact acataatgcc ccttcgctaa
     3061 ctatcgaggc cagatggaac acgccggtgc gcgtcaagtg gatcaacgat ctaaaggacg
     3121 cggctggcaa ctatcttccc cacctcctga cggtagatcc gaccctgcac tgggccaacc
     3181 cgcccggggg tctaacaggc cgcgacacac gtccgacctt caccagcacg cccggtccgt
     3241 acaccggtcc ggtgcccatt gtcacgcacg tgcatggcgc tgtgggcgtg ggcgacgaga
     3301 gcgacggtta cgccgaggcc tggtatctgc ctgctgcggg caacatcccg gctggttatg
     3361 ccaccgaagg cacctggtac aatttcttca aaggcaaggc tgcaacggcg catgaggtca
     3421 catggggttc gggctttgcc attttccagt atccgaacgc gaatcgcgcc tcgaccgtct
     3481 ggtaccacga ccacgtcctg ggcatgaccc gcctgaacgt gtatgccggt cccgccggct
     3541 tctacattat ccgcggcggt ccggatggcg acgatgccgt gctcgacagc cgatccggta
     3601 gaccagccgt gttgccggga ccggccccca aggagaacga ccaattccca cccaataaga
     3661 cctactacga gatccccatg gcgattcagg atcgctcctt caacaccgac ggatctctat
     3721 tctacccgaa cacgcgggaa ttcttcgacg agatcgtcgg cgacta
//
LOCUS       DASUTG010000174         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1657709, whole genome shotgun sequence.
ACCESSION   DASUTG010000174 DASUTG010000000
VERSION     DASUTG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657709"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF089_03905"
     CDS             <1..504
                     /locus_tag="VF089_03905"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX6173134.1"
                     /translation="VTIFASILAPIGTPLIGTVMLGNLMKESGVVERLKSASENEIAN
                     IITLLLGLTIGATMEAHKFLRGETLLVLGLGLVAISLDTVVGVLFGKLMCLLTGGKVN
                     PLIGAAGISAFPMAARVAQTEGQKYNKKNYLLMHAMSANAGGQIGSVIAAAVMLSVLT
                     GMGIVGG"
     gene            515..2068
                     /locus_tag="VF089_03910"
     CDS             515..2068
                     /locus_tag="VF089_03910"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX6173135.1"
                     /translation="MAKSRDDLENRLTALEKKAVAGAVKAAGKQPDEGRLTARERVAT
                     LLDPGTFVEEFMLAETQCTDFGMAERKQPSDGVVTGYGKVDGRAVYVFSQDRSIMAGS
                     VGSAHAEKIAYVIQTARRLGVPLIGLNDSAGARIQEGLSVTSAIGKIFFENSIASGCI
                     PQISAIMGPCIGVGSYSPALTDFVLMVQNSSQMFITGPAVIKEVLGETVTMEELGGAK
                     IHSEVSGVADLVTKNDQECLATIRRLLGFLPSNFESPPPRKSTSDPPDRSLDALNQIV
                     PDDMRRAYNILQVIKAIVDDGDFLELKAKFARNLVIGFGRVDGHPVGFVANQPMFLAG
                     SLDIDASDKAARFIRFCDAFNVPIITLMDVPGFFPGKQQEEKGIIRHGAKMLYAYAEA
                     TVPKVTVVLRKGYGGAKQALCTREMGADQLFVWPGVELAVMGGGGAVNVLYKREIERS
                     ADPEQTRREKIAEFQERFSGPFEALSKRFAHAAVRPAETRQRLIRSLEILRNKKEERP
                     KKKHGLMPV"
     gene            complement(2095..>2710)
                     /locus_tag="VF089_03915"
     CDS             complement(2095..>2710)
                     /locus_tag="VF089_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6173136.1"
                     /translation="EVMLEGMFGPVTEEQKKWLLRVQANSHNLINLVSDFLDLSKLES
                     GYVNVSKQVVSVVDLVRKSIESFQVVASNKNVTVAEAIDASLPSIFADPQRLEQVLNN
                     LISNAIKFTGDGGEVKVGAALAETRGIRVWVQDSGEGIPTDEIGELFQKYRQGGNANN
                     SNHKGTGLGLVICKMIVEAHGGTIWVESEQGQGSIFSFSLPVEA"
BASE COUNT          612 a          719 c          740 g          639 t
ORIGIN      
        1 gtaacgattt tcgcttcgat cctcgcgccc atcggcacac ctttgatcgg cactgtcatg
       61 ttaggcaatc tcatgaagga gagcggtgtc gtggagcggc ttaaaagtgc ttcggaaaac
      121 gagattgcca atatcatcac tctgcttttg ggtcttacga tcggcgccac aatggaagcg
      181 cataagtttc tgcggggtga gacattgctt gttttgggac ttgggcttgt cgccattagc
      241 ctggataccg tcgtcggggt gctctttgga aaactcatgt gtctacttac cggcggcaag
      301 gtcaatccgc tgatcggcgc ggcagggatt tcagcatttc caatggccgc ccgggtcgca
      361 caaaccgaag gtcagaaata caacaaaaag aattatttat tgatgcacgc gatgagcgcc
      421 aatgccggtg ggcagattgg ttccgttata gcagccgcgg tgatgctctc ggtgctcacg
      481 ggaatgggaa ttgtcggcgg gtgacaggga cgttatggct aaatcgagag acgatcttga
      541 aaacaggctt accgcgttag aaaaaaaagc cgtcgcgggc gctgtaaagg cggccggcaa
      601 acaacccgac gaaggccgac ttaccgcgcg tgaacgagtt gcaacgcttc ttgatcccgg
      661 cacgtttgtc gaagagttca tgctggcgga aacgcaatgt acggacttcg gcatggcgga
      721 acgaaaacag ccgtccgacg gcgttgtgac gggttacgga aaggtcgacg gccgcgcggt
      781 ttatgttttt tcacaggatc ggtccatcat ggccggcagt gttggcagcg cccacgccga
      841 aaagatcgcg tacgtcattc agactgcgcg taggctgggc gttcctctga tcggtcttaa
      901 tgactcggcg ggagctcgaa ttcaggaggg actttccgtc acttccgcca tcggcaagat
      961 ctttttcgag aacagtatcg cctccggctg catcccgcag atctcggcaa tcatgggacc
     1021 gtgtatcggt gtcggctcct attcgcctgc gcttacggat ttcgttttaa tggtccagaa
     1081 ttccagccag atgttcatca cgggtccggc ggtgatcaaa gaagtactgg gcgagaccgt
     1141 caccatggaa gagttgggtg gcgccaagat tcactccgaa gtctcaggcg tcgcggacct
     1201 ggtgaccaag aacgatcaag aatgtctcgc gacaattcgc cgcctgctcg gtttcttgcc
     1261 atctaatttt gaatcgccgc cgccgcgcaa atcgaccagc gaccctccgg atcgcagcct
     1321 cgatgcgcta aaccaaatcg tcccggatga catgcgccgc gcctacaaca ttctgcaggt
     1381 catcaaagca attgtcgatg atggtgattt tcttgagttg aaagcgaaat tcgcgcgcaa
     1441 tctggtgatc ggcttcggca gggtggacgg gcacccggtc gggttcgtcg ccaaccagcc
     1501 aatgtttctc gccggcagtt tggatatcga cgcgtctgat aaagccgctc gtttcattcg
     1561 attctgcgat gctttcaatg ttcctatcat cacactgatg gacgtacccg ggtttttccc
     1621 tggaaaacag caagaagaaa aaggcatcat tcgccacggc gctaagatgc tctacgcgta
     1681 tgccgaggca acggtgccaa aagtaactgt tgtcttacgc aaaggctatg gcggcgccaa
     1741 acaggcgctc tgtacccggg aaatgggcgc cgatcagttg ttcgtttggc cgggtgtcga
     1801 gctcgccgtg atgggtggag gcggtgcggt caacgttctt tataagcgag aaatcgaacg
     1861 atcagcggac ccggagcaga ctcgcagaga aaagatcgcc gagttccagg agcgcttcag
     1921 cggccccttt gaggctttgt ccaaacgatt cgcgcacgca gccgtgcgtc cggcggagac
     1981 ccgtcaacgg ctaatccgat ctttggagat actgcgcaac aagaaagaag agcgaccgaa
     2041 gaagaaacat ggtctaatgc cggtgtgatt atgaatagaa ttcggctcga ggggttacgc
     2101 ttcaaccggc agtgagaatg aaaagatgct cccctgcccc tgttcacttt cgacccagat
     2161 tgtgccgcca tgagcttcta cgatcatttt acagatcacc agaccgagac cggtgccttt
     2221 gtgattggag ttgttggcgt ttcccccttg tcgatatttc tggaaaagct cgcctatttc
     2281 gtccgtgggt atgccttcgc cactatcctg aacccagacc cgaatccctc ttgtctcagc
     2341 caaagctgct ccgaccttta cttctccgcc atcgccggta aatttaatcg cattgctgat
     2401 aagattattt aagacttgct ccaaacgctg cggatcggca aagatcgatg gtagcgaggc
     2461 atcaatcgcc tctgcaaccg tcacattttt gttggatgcc accacctgaa agctctcgat
     2521 gctttttcga accaagtcca cgacactgac gacttgcttg ctgacattga cgtagccaga
     2581 ctcgagtttg gaaagatcta gaaagtcgct cactaggtta attagattgt gactgttggc
     2641 ttgaacccgt aacaaccatt ttttttgctc ctcggtcacc ggtccgaaca tcccctccag
     2701 cattacctct
//
LOCUS       DASUTG010000175         7488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_27841, whole genome shotgun sequence.
ACCESSION   DASUTG010000175 DASUTG010000000
VERSION     DASUTG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27841"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF089_03920"
     CDS             <1..395
                     /locus_tag="VF089_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6173137.1"
                     /translation="LKNEAAKLAHGAGSFVLGGIDDLILGKPDWPRVMSKFGAPHILL
                     SHNPDHFYDAARRGVSLVLSGHTHGGQIRLPLGPPIIRQSRFCLDEGAYSFESSLLVV
                     TRGLGSILLPWRWGADPEAVMIDVVAAK"
     gene            complement(413..1837)
                     /locus_tag="VF089_03925"
     CDS             complement(413..1837)
                     /locus_tag="VF089_03925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173138.1"
                     /translation="MSAKINYLLLTTALLATFFSGTATRIVSISMPTIASSLATDLLG
                     VSWALLSYQLSNIGLSIVFGRLSDLWGREKVFALGFLVFSAGSFLCGLSQSLIQLILF
                     RFLQGIGAAMLQSSSRALAAESVPENLAGRAQGYMTTAHHVGFIIGPSIGGVMIDYLS
                     WRWSFFFLVPIGICGMLLTLLNVKRRPAVVQGRSASIDYLGAALLFATTSTLVMLLDR
                     RTQQMIPTSSKIGLGVLFIACLVGLILHETRTKNPILNLTLFRIRRFSMSVISLLVVA
                     SCYALTGFLLPFYLQGVLGLTATSIGLVFMAPSVITVALAPASGYLADRLGPRIPASV
                     GVAFMIVSLGIGAFLRVDSHWLLPALLVIVSAITNGIFNPANSMAMIAMMPAEHRGFA
                     SAVNHVTFGFGNVVGVALSSLLMSLAFEHHTGIAGTALTTANPSGFVAALNTTFIIAA
                     CWSSVGLATSLAGSARRSSHPGSR"
     gene            complement(2094..3167)
                     /locus_tag="VF089_03930"
     CDS             complement(2094..3167)
                     /locus_tag="VF089_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173139.1"
                     /translation="MNNLRFITLTILFLIRAPLADHAQAQGVKAGGGFDEKAVADFYR
                     GKTIRILIGSGPGGTYDIYSRLISRHMSRFIPGNPTLLVQPRAGAGGLIAANAVYNAE
                     PKDGTVIGSFGETFPLRQAMGAPGIEFDSARYQWIGSAVNTAIACVGRTDSPVSSFHD
                     ITAGKPFTVGTMAPGSTIYDTPAIINAALGTQMKLVRGFEGVASIVNAAESKEVDGYC
                     ASWLAMLTTGRHLQLINGGTLKPLLIMGDKTPDHPLMKGVPAAETVAKTEEAKLLLRA
                     MHAPSEITNPYVVHPDVPKERVEALRKAFSATFADAEFISDAKKSKIEFSPSSGERVT
                     EVVHSILKTPPAILAKMKKILVQ"
     gene            3624..4460
                     /locus_tag="VF089_03935"
     CDS             3624..4460
                     /locus_tag="VF089_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173140.1"
                     /translation="MLKPTGLVGGHYECRSLQQTLPILQDLLALEVVTRDDGETVLKH
                     PNTGWKLIAHESGPDASDKPFQHHYGVRVATNREIDEAHDYLQSKKREYKIKIIQPKE
                     NHNAYSVHFFEPGGNFWEIESYEKADEAGMGKTTSPHWQTPISEQMFPGRGYIPQALT
                     HGTVQFHDIDATRRFYENVLGLEVVQLWPSSIYVKHPESPWYIVNLPFRAEPILPTRS
                     QRFVLSLESASKVEEAHHWFSSSAERLGLSTLDPVQRSGDVASFIFSDLNRNWWEVTS
                     AR"
     gene            4734..5069
                     /locus_tag="VF089_03940"
     CDS             4734..5069
                     /locus_tag="VF089_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6173141.1"
                     /translation="MSEFNRAIAYSELDEDSILAVKINGERIALYKVGGEVYATSEMC
                     SHSDCSLEDFGKIVEKDQVECICHGARFRIKTGEVTRLPAEVPLKTYSVRIDNDEVWV
                     RVPDPVKNS"
     gene            complement(6721..6927)
                     /locus_tag="VF089_03945"
     CDS             complement(6721..6927)
                     /locus_tag="VF089_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173142.1"
                     /translation="MAVDPPLLLLVELIASLDAARRNELRELLQGLMGEGRTLMIATH
                     DEEFASTCATRVLRMNNGIVSSQD"
     gene            complement(6893..7273)
                     /locus_tag="VF089_03950"
     CDS             complement(6893..7273)
                     /locus_tag="VF089_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173143.1"
                     /translation="MRPDRVVRFDVEIEIIAQGINRSATFLNVGFTSIDQSIERGDAE
                     IGLGGIEDTQGRRATMAPTVPYYRFREVLSVRDTDPSRFRTLADLRGVENRANALLHE
                     LWVGRRSGCDRARFGCRSAAAAAG"
BASE COUNT         1865 a         2096 c         1909 g         1618 t
ORIGIN      
        1 cactgaagaa tgaagctgca aaattagccc atggcgcagg aagctttgtg ctcggcggca
       61 ttgatgatct tatcctcggc aagcccgact ggccgcgcgt aatgtccaaa ttcggcgctc
      121 cgcatatact tttatcgcac aaccccgatc acttttatga tgccgcacgt cggggagtgt
      181 cgctcgttct ttccggacac acgcatggtg gtcagattcg cttgcctctt ggcccgccca
      241 tcatccggca aagccggttc tgcctggacg aaggagctta ttcttttgaa tcctcgctgc
      301 tggtagtcac acgtggtctt ggcagcatct tgctgccgtg gcgctggggc gctgatcccg
      361 aggcggtcat gatcgacgtc gtcgcggcaa agtagtcgcg catggcccgt gattaacgcg
      421 atccaggatg ggaagatctg cgagcgctgc cggcgagaga agtggccaac cctaccgagc
      481 tccagcaggc ggcaatgata aatgtggtat tcagagccgc aacaaatccc gagggatttg
      541 ccgtggtaag agcggttccg gcgattccgg tatgatgttc gaaagctaac gacatcaata
      601 aactgctcaa cgccacgccc acgacattac cgaaaccaaa ggtaacgtga tttaccgccg
      661 atgcgaaacc gcggtgctcg gccggcatca tcgcaatcat tgccatagaa ttggccggat
      721 tgaaaatccc attggtgatc gcactgacaa taaccaacag cgccggcagg agccaatggg
      781 aatcgacacg caaaaaggca ccgatgccaa gcgaaacaat catgaacgcc accccgacac
      841 tcgctggaat acgcgggccg agacgatcgg ctagataacc gcttgcaggc gccaatgcta
      901 ccgttattac cgacggcgcc ataaatacca accctatgct cgttgccgtt aaaccgagga
      961 cgccttgaag gtaaaacggc aggagaaatc cagtcaacgc gtaacaactg gcgacgacca
     1021 gcaagctgat cacgctcatg ctgaacctgc ggattctaaa caacgtcagg ttgagaatcg
     1081 gattcttggt tctggtttca tgcagaatca acccgacaag gcaggcaata aacaaaacgc
     1141 ccaacccaat cttcgagctc gtcggaatca tctgctgtgt gcgccggtcg agcagcatca
     1201 ccaaagtgct ggttgtcgca aacaacaaag ccgcgccgag ataatcgatg gaagccgagc
     1261 ggccttggac taccgctgga cgtcgcttta cattcaacaa ggtcaagagc atgccgcaga
     1321 tgccgatcgg caccagaaag aagaaactcc agcgccagct caggtagtcg atcatcaccc
     1381 cgccgatgct tgggccaatg ataaacccta catgatgcgc cgttgtcata tacccctgtg
     1441 cccgtccagc aaggttctcc ggaaccgatt cggccgccaa ggcccggctg gaggactgca
     1501 acatcgcagc gccgatgccc tgcaaaaaac gaaacagtat gagctgaata agcgattggg
     1561 aaaggccgca taaaaaggac ccagcgctaa ataccagaaa gcctaaggca aaaacttttt
     1621 cccggcccca gagatccgag agacggccaa aaacgatcga gagcccgatg ttcgataact
     1681 gataagagag cagcgcccag gaaacgccga gcagatcggt tgccaagctg ctcgcaattg
     1741 tcggcatcga gatgctgacg attcttgtcg ctgtcccgga gaaaaacgtt gccagcaatg
     1801 ctgtcgtaag aagtaaatag ttgatctttg cgctcatcac gatgcttaag tttcaacaca
     1861 ggatctcgaa tccatgtgac actccgaccg gcaagtagaa gcggccccat ggattgcgac
     1921 tttctgctgc tatagcggat tccgaccctg ttgattttgt tcccgtgctc tccggcagga
     1981 aaaatttcca agaaaacagt acagcgaagg attggttcgc gagagaatcc ctcctaagag
     2041 ggaccggcaa ctcgaagcgg tccggccgag gttttactat tcgatggact cgctcactgg
     2101 accaaaattt ttttcatttt tgcgagtatc gcgggtggag ttttgagaat gctgtgaacg
     2161 acttcggtca cacgttcgcc gctgcttgga gaaaattcaa tcttgctctt cttggcgtcc
     2221 gagataaatt ccgcatcggc aaaagttgct gaaaatgcct tgcgcaacgc ttctacccgt
     2281 tctttgggaa cgtcggggtg aaccacgtac gggtttgtga tctccgaggg ggcgtgcatc
     2341 gcgcgcaaca gtaacttggc ttcctctgtc ttagctaccg tttctgcagc gggaactccc
     2401 ttcataagcg gatgatccgg agttttgtcg cccataatca gaagcggttt cagtgtgccg
     2461 ccgttgatca actgtaggtg cctgccggtg gtcagcatcg ccagccaact tgcgcaataa
     2521 ccgtctactt cctttgactc cgcggcgttg acgatgctgg caactccttc gaatccccgc
     2581 accagcttca tctgtgttcc aagagccgca ttgatgatcg ccggtgtatc gtagatggtg
     2641 ctgccaggcg ccatcgtgcc caccgtgaag ggcttgccgg ctgtgatatc gtgaaatgac
     2701 gacaccgggg aatcggtccg tccgacgcat gcgatcgcag tattcacggc gctgccgatc
     2761 cattggtacc tcgccgaatc aaactcaatc cccggcgccc ccatggcctg ccgcaaggga
     2821 aaggtttcgc cgaacgagcc gatcacagta ccgtccttgg gctcagcatt gtaaaccgcg
     2881 tttgcggcga taagaccgcc ggctcccgcc ctgggctgaa ccaagagggt aggattgcca
     2941 ggaataaatc gcgacatgtg gcgcgagatc aggcgcgagt agatatcata ggtcccgcca
     3001 ggacccgaac caatgaggat tctgatggtt ttacctcgat agaaatctgc cacggctttt
     3061 tcatcaaaac ctccgccggc cttgaccccc tgcgcctgtg cgtgatcggc cagcggagca
     3121 cgaatcagaa aaagtatcgt caaagtgatg aatcgaaggt tattcataga ctcctcgcat
     3181 ctcactagag tactcatcct gctacttcta tagagaccgg ccaggaactt ctgttctcat
     3241 ctgctcaatt caatctcgaa gatcggtaaa aaaacccgag acattcatca tcccaatgaa
     3301 ggtttcgctt gcatctctca actccagcgc agcctttttg ctcgttcaag cccctatttc
     3361 acagaacgac ccggatgacc caacaggccg cgacgtgcat taaaagccgg taaagctcag
     3421 ccgttcacac ctggaattcg agtcgagaaa aaaccggttg cagaatattt aggaaccctt
     3481 catccgtcaa gataaatcag cgcgcgccgc aatcgaccat gagccaggct tacgcctacc
     3541 gcatgtcgtt gcgatgcctc ttgtaattat gttacggcaa atcagagccg ctttcagtcg
     3601 attgtgcaca aaggaggagg atcatgttga aaccgacagg actggtgggg ggccattatg
     3661 aatgccgttc actgcaacaa accttgccta ttctgcaaga cctcctggcc cttgaagtgg
     3721 taacgcgaga cgatggtgag accgtcctca agcaccccaa taccggttgg aagctgatcg
     3781 cgcacgagtc aggccccgat gcttcggata agccgttcca acatcattac ggcgtgcggg
     3841 ttgcgaccaa ccgtgagatc gatgaagcgc acgattacct gcaaagcaaa aagcgagaat
     3901 acaagataaa aatcattcaa cctaaagaaa atcataatgc gtactcggtc catttctttg
     3961 aaccgggagg aaatttctgg gaaatcgaat cttacgagaa agccgatgag gccgggatgg
     4021 gtaaaactac gagccctcac tggcaaactc cgatcagcga acagatgttt ccagggcgcg
     4081 ggtacattcc ccaggcgctg acgcatggca cggtccagtt tcacgacata gacgccaccc
     4141 ggcgtttcta cgagaacgtc ctcgggttgg aggttgtgca actgtggcct tcctccatat
     4201 atgtgaaaca tccggaaagt ccgtggtaca tcgtcaattt gccttttcgc gccgaaccga
     4261 ttctgcccac tcgatcgcaa cgttttgttc tttcacttga gtcagcatcc aaagtcgaag
     4321 aggcgcacca ctggttctcg agttccgctg agaggctggg actgagtaca ctggatccgg
     4381 ttcaacgttc gggtgacgtt gcttcgttca ttttctcgga cctgaaccgc aactggtggg
     4441 aagttaccag cgcacgctga gaatccaaat tccccgatgt cagtcggaga tttgaaaaag
     4501 acccatatgc ttcgttgccg ctcaatcgcc tcgctgcaag gtactgtaag tacgcctctg
     4561 ctcgtcgatt tttcgcgcgc ctcgcacctg gatctttttg agcagcctgc aagaggcttt
     4621 ttttcagcac cttgctagtg gctgcggcag aatgaatttc gtcgcgggtc ttcggttcaa
     4681 tcgatcctgc ctggattcat tttgccgacc agctggacaa tacgaggaaa aaaatgtctg
     4741 aattcaacag agctatcgca tactccgaac tggacgaaga ttcgatcctg gcggtcaaga
     4801 tcaacggcga gcgcattgcg ctttacaaag tgggcggcga agtctatgcc accagcgaaa
     4861 tgtgctcgca tagcgactgc tcactcgaag attttggtaa gatcgtcgag aaggatcaag
     4921 tcgaatgcat ctgtcacggc gcaaggtttc gcatcaagac cggtgaagtg acgaggttac
     4981 ccgccgaggt tccactaaaa acctattctg taaggatcga taatgatgag gtttgggtcc
     5041 gagtgccgga tcccgtcaaa aactcatgag gacacaatcg gcgtttttct tttttcgcag
     5101 cacacgcgct gattgaaacc ctgagtatct tttgatcgtc agtattcttt ccgattgccg
     5161 ctctcaaacc gagcacatgg ccagcgcgga agaattttaa caacactcat cagcgaacag
     5221 ccgagccgac ccatcggtga ttttgataaa acttcaagcc tgccgtcgtc acgcccattc
     5281 cagccaaagc tccattaaca tcgaccatct attcaaaaga tcgagggacc gaatgcgcgg
     5341 cttcgatcgg cggccggagt agctttgagg acgcagaagg cttcaagcag ctcccgctga
     5401 tttctaccga ctacaaatca gagtcgcacg cgcaagggcc acaattcctg aaactgccaa
     5461 agaacgcttc tatccctaca actcgctttt tggaagagaa cccgcaagat ttctgtttga
     5521 aatgaactac gccagcgctg caggagatgc ccttcgatcg cgcgccttta tcctgagccg
     5581 aggcctcatc gcacaaacgc atatgaaaag atctccatag gtagaactgc gcaccgcagg
     5641 atctaacccg ttcttattgc ggtttgtggc acctcgcttg cgtgacacgc caacccgaca
     5701 tcgaaacact tagtgctctg ttatggaact ccaaaccgct gagaagattc atacggcggc
     5761 tccaataaag gcatcagaag aggcctcaag attgatgcca acagactcta gctcggcaga
     5821 aatcagtttc gcagcgtgtc attgatccac cttattcaaa aatgatgttc agtacggcat
     5881 ttcattatcg ccgaacgatg taagcaacag acaactctcc tcatcagcga tgacggcagc
     5941 ggcattccca cgtttaactc caacggcatg gataactcgg ttggcttctg tcgcagttgt
     6001 aaatttcaag ctgccgagcg gcaacaccct ggagcattca tgactaagaa gaatcgcaat
     6061 ctaacaggta cggcatgcgc aatacaatcc tcaactgcgc ataagacctt aagcggaaac
     6121 acattcgctc gcagaatcac gtcgatactt gtggcgttgt tatcatttcc gacggtagcc
     6181 ttcggctatc cttttcacct cacttggacc gacataaacg cagacgagga cggcacaata
     6241 gtgtatcggg agatatctgg tgtttttcat gaagtgggct tcgtcggtcc caacgtaacg
     6301 agttttaccg atgaaccgcc cggagccgac ggtgatacat tttgctggac cgtgcgggct
     6361 tttcgtgaag gggttatttc cggatcgtcc aatgaaacgt gcgcgaccgt accaacaaca
     6421 tcggtaaatc cacgacctcc aagccagggc agcgcaaagg cgaaagacag tagcctccat
     6481 ctgagatgac gactgtcggt gtaagtttaa cccgctccaa gtaacgctga aaggcgaccg
     6541 ccgagcgaag cgcggcacaa ctcaagacct cgcgcactag tggaatttag tagcgataag
     6601 aaatgatcgt cgcggcgaca tttgccgcag tatcagcatc aaaatgatta gccggtctga
     6661 tcgccttccg gcgcatggga ttgttcgtat gcaagagcgg agacgctagc tgtagctact
     6721 tcaatcctgg ctagaaacga taccgttgtt catgcgtaaa acccgcgtgg cgcaggtgct
     6781 cgcgaactct tcatcgtgag tggcgatcat caaggtgcgc ccttcaccca tcaatccctg
     6841 aagcaactct cgcaattcgt ttcgtcgcgc cgcatccagc gatgctatca gctcaaccag
     6901 cagcagcagc ggcggatcga cagccaaagc gcgcgcgatc gcacccgctg cgcctcccca
     6961 cccatagctc gtgcagcagc gcgttggcgc gattttcgac gccgcgcaga tcagccaggg
     7021 tgcggaagcg tgaagggtcc gtgtcacgta cactcaacac ttcacgaaat ctataatacg
     7081 gcaccgtcgg ggccatcgta gcgcgacgcc cttgcgtgtc ttcgatccct cccaagccga
     7141 tctcggcgtc gccgcgttcg atggactgat cgatggatgt gaaaccaaca ttgaggaagg
     7201 tggctgaccg gttgataccc tgcgcgatga tctctatctc gacatcgaac cgcaccactc
     7261 gatcaggacg caccggatcc gcttggacaa gcggcgcgcc acccccagtg tccgccgccc
     7321 aacgaattcc tgatgcgccg aggccgcaat cgtcaacgac atcggcgtca acatagcgaa
     7381 gctcacaaca atgccaacat tgccaggttc gaaccgcaaa tctgccggct atctactctg
     7441 cacgaagtct tcaagactaa atttccgtca gagttgcggc agcgggca
//
LOCUS       DASUTG010000176         6148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_28063, whole genome shotgun sequence.
ACCESSION   DASUTG010000176 DASUTG010000000
VERSION     DASUTG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28063"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1323)
                     /locus_tag="VF089_03955"
     CDS             complement(43..1323)
                     /locus_tag="VF089_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6173144.1"
                     /translation="MQLTLACHPITEIRFGSQTRIDGTVLVVDAAELRRLVLEDEALA
                     AVDFDIVRPGDSCRAGPIYDIVEPRAKAPGSGPDFPGILGAAMTAGMGMTHVLAGSAV
                     SVLAEMSPGEGRSATGRVLEMSGPLVSASNYSSLQHLLTIPHSKPGLPRQVVLKAYRL
                     AALKVAVQIARCAIDQAPASMQSFPSIGPWLQDRSDLSRVAYVGQVFSRQRKPGVDEP
                     ILYGANTDGMLPMMLHPDEWLDGAIVPSLNSWFGGTETYFYQNHPVILELYRRHQARE
                     INFVGTIATIAGSDHFDRERQCRAAANLTQWNLRADGAVLTKYGGGLPHADLAETARL
                     LEGMGIRTAVMVSDSSRDRRVESALLFNFPEVNAVVYNGGNGTEWEVPRVDRVIAATP
                     ELAELLVGPISLEASNIIGVTNQQGASRMTSLVY"
     gene            1491..2996
                     /locus_tag="VF089_03960"
     CDS             1491..2996
                     /locus_tag="VF089_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX6173145.1"
                     /translation="MIRFSPIPNRAHLVHWHEWEDHPFGLARNQDKPVMLFLTAFWCR
                     FCQRMDEEAFSDNENLALLNAYFISVRCDNAQRPDIDIRYNSDGWPTIAFMTADGILL
                     SVANYLPSEQFGDLLVRVYTAYHEKKDQIRLLHRNAQQESSDMPSCVNDPRPDLSMID
                     DITNQMIELADREYGGYGRGQKFIQPQVNDFLLARYEATKESQYLDHVCLTLDRMRES
                     PMRDHETGGFFRTCSGRDWSRPHREKLLAEQAGVLANCLRAFRITRLPVYARMAEEQI
                     TYLNAHLFDAATGAFSGCEDLLRTESGGLSSDEEFFSILDGCIYIDANAKTIVTYLDA
                     AATLPEPGCRERALHALEFLWERCRTPNGAMTHYYDGAAHLAGLLEDQVYMGNALLKA
                     QSITRDTRYLARATALAEFILARLKNPHGGYYDIDIPGPALSAFRLTLIAQNGRAASF
                     FLALAEATQDSRYRDAALWALAAFNGDFSRYGLDAACFGQALGEYAMGAQT"
     gene            complement(3115..3795)
                     /locus_tag="VF089_03965"
     CDS             complement(3115..3795)
                     /locus_tag="VF089_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6173146.1"
                     /translation="MDVALIKSDVVKASHILHQQGVAAAFGHLSARIPETDTFIFPPR
                     MSPALVRADNLLVLDVEGNQLSGDGRPNTEFWIHARIYKARPDVQAICHVHPPSCVVL
                     SSLGETIRPLHASGAIFRNDVQVFDRISLIRTRELGDAVARTLGDHGAMLLRGHGVNV
                     ADKDVRRVCVMTLWMEEAANYQLRAMSAGKPRYFTAEELETIYPQVSGEEVSKRVWEY
                     FSSRISTG"
     gene            complement(3851..4144)
                     /locus_tag="VF089_03970"
     CDS             complement(3851..4144)
                     /locus_tag="VF089_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEX6173147.1"
                     /translation="MGRRYFEITEVIKVCKVDKDFIQQLERESVIRPVLQRKRKLYPP
                     DQVDRIRIANLLIQEMGVNFEGAEVVLNMREQMIAMQRQFNEVLRLIGDEIKR"
     gene            complement(4157..5284)
                     /locus_tag="VF089_03975"
     CDS             complement(4157..5284)
                     /locus_tag="VF089_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX6173148.1"
                     /translation="MAERNFYQILGVERGASHDDIRKAYRKLARKHHPDINPGNKEAE
                     NKFKEIATAYDVLGDEQKRKLYDEFGEAGLAAGFDADKARSYQQWQQQSAAGAGGGYE
                     FDMDDLSELFGGFGGMGARTRRSRQGPMRGEDIESSMDIDFLDAVRGFQTSITLQRPV
                     TCDTCTGSGTKPGSNPSQCPECGGSGSKSVAQGPLQFRQTCPRCMGTGKLPGDPCAVC
                     RGSGRVLRPETIRVNIPPGAEPSKRIRLRGKGEAGVRGGPPGDLYIVPRIRPHPLLTR
                     SGKDLSMELPITIGEAVRGATVEVPTPMGKLKVKIPAGAQSGQRLRLKGKGVSAHGQS
                     PAGDLYLQLMIRVPTDNNIAADVIDKIDRAYADDVRKDVRL"
     gene            complement(5370..5591)
                     /locus_tag="VF089_03980"
     CDS             complement(5370..5591)
                     /locus_tag="VF089_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173149.1"
                     /translation="MAVSSGKFRPSTCLIQARAMQFEQLGSSYVLVLGLEALLAVILG
                     TVLFGEQLSGRAAAGIALVVIGILLIRLT"
     gene            5939..>6148
                     /locus_tag="VF089_03985"
     CDS             5939..>6148
                     /locus_tag="VF089_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173150.1"
                     /translation="MRNKTGTTEYKEQRGTGSRRRIWIAIGVLVALLGMAAAWRWTPL
                     ADQIDIGKVSAWAAALRHNPSRVMII"
BASE COUNT         1329 a         1792 c         1671 g         1356 t
ORIGIN      
        1 attgagcggt aacgaagcat atgggtcttt ttcagcaacc tgctagtaaa ccagcgatgt
       61 catacgggag gccccctgct ggttggtaac accgattata ttcgaagcct caagcgagat
      121 cgggccgacc agaagttctg ccagctccgg agttgccgcg atcacccggt ccacccgcgg
      181 gacttcccat tcggtaccgt tgccaccgtt ataaacaacc gcgttgactt ccggaaagtt
      241 aaacagcagc gccgactcga cccggcgatc tcgcgacgaa tcggaaacca tcacagcagt
      301 tcgtatgccc atgccttcta gcaaccgcgc cgtctctgcc agatcggcat gaggcaagcc
      361 gccgccgtat ttggtcagca ccgcgccgtc agcgcgcaag ttccactggg tcaaattcgc
      421 agcggcacga cactggcgct cccgatcgaa gtggtccgaa ccggcgatgg tcgctatggt
      481 tccgacgaaa ttgatttccc gcgcctgatg gcgccggtaa agctccagga tgactggatg
      541 gttttgatag aagtaagtct cggtgccgcc gaaccaggag ttgagcgatg gcacgatagc
      601 gccgtcgagc cattcgtccg gatgcagcat catcggcagc ataccgtcgg tgttggcgcc
      661 gtagagaatc ggctcatcga caccgggttt gcgctgacgt gaaaaaacct gcccaacgta
      721 ggccacacgt gaaaggtcgc tgcgatcttg caaccagggg ccgatggatg gaaagctttg
      781 catcgatgcg ggagcttgat cgatcgcgca ccgggcaatc tgtaccgcaa ccttcaaggc
      841 ggccaggcga taagccttca ataccacttg ccggggcaac ccgggcttgc tatgcggaat
      901 agtcaacaga tgctgtaaag aggaatagtt gcttgccgac acgagcggac cgctcatttc
      961 caaaacgcgt ccggtggcgc ttctcccctc cccgggtgac atctcggcca ggacagatac
     1021 cgcagacccg gcaagcacgt gggtcatgcc catccccgca gtcattgcag cgccgagtat
     1081 tccgggaaag tcgggacccg agcccggcgc tttcgcgcgc ggttcaacaa tgtcgtaaat
     1141 ggggccagcc cggcaactgt cgcccggccg gactatgtcg aaatcgacag cggcaagcgc
     1201 ctcatcttca agcaccagcc ggcgcagttc ggcggcatca accaccagca ccgtgccatc
     1261 aatccttgtc tgggaaccga agcggatttc ggttattgga tgacatgcga gagtaagttg
     1321 catagattgt ctttcggtga atacaagcaa attgatcaac tcgatcttat ggccgggcca
     1381 ttaggaatgt caacgcgacc cagcgcttga tttttggtgc ctcttctctt cttgtacgac
     1441 gccaatggtg ataacaagcg cttatcggat taatgccgaa gaattccgct atgattcgct
     1501 tttcacccat cccaaatcga gcacacctgg ttcattggca cgaatgggag gaccatccgt
     1561 tcggcctcgc tcgtaatcag gacaagccgg tgatgctttt cctcaccgca ttctggtgcc
     1621 gcttctgcca gcgcatggac gaggaggcat tctcggataa cgaaaatttg gcactcctca
     1681 atgcttattt tatttcagtc cggtgcgaca acgcccaacg acccgatatc gacattcgct
     1741 acaatagtga cgggtggccg acaattgctt tcatgaccgc tgacgggatc ctgctttctg
     1801 tagcaaatta tctcccgagc gagcagtttg gcgatctcct ggttcgcgtg tatacggctt
     1861 atcacgagaa gaaggatcaa attcgactgc ttcaccggaa cgcgcaacag gaaagcagcg
     1921 atatgccatc ctgcgtgaat gacccacggc cggatctttc gatgatcgac gatatcacga
     1981 accagatgat cgagctcgcc gatcgggagt acggcggtta tgggcgcgga caaaaattca
     2041 tccagcccca ggtcaacgac ttcttgctcg cccgttatga ggcaaccaaa gagtctcagt
     2101 acctggacca cgtttgcctg accttggatc gcatgcgcga gagtccgatg cgcgaccatg
     2161 aaaccggcgg cttttttcgc acctgctccg gccgcgattg gagccggccg catcgcgaga
     2221 aactgctggc ggaacaggcc ggcgttctag cgaactgcct gcgggcattt cgaattacgc
     2281 ggctgccggt ttacgcgcgc atggccgaag agcagatcac ctacttgaac gcacacctgt
     2341 tcgatgccgc tacaggggct ttctccggct gcgaggatct tctacgaaca gaaagcggcg
     2401 ggttatcttc cgatgaagaa tttttctcga tcctggacgg ttgtatttat atcgatgcca
     2461 atgcgaaaac cattgttacc tatctcgacg ccgccgcaac tctccccgag cccggctgcc
     2521 gggagcgcgc gctgcatgcg ctggagtttc tctgggaacg ttgccggacg ccgaatggtg
     2581 cgatgacgca ctactacgac ggcgcggcgc accttgcggg attgctcgag gaccaggtct
     2641 acatgggaaa tgcgctgcta aaggctcagt cgatcacccg agatacccga tatctcgcgc
     2701 gcgccacggc tctagcggaa tttattctgg cgcgcttgaa aaatcctcac ggcggctact
     2761 acgatatcga cataccgggt cctgctctca gcgctttccg cctgaccttg atcgcgcaaa
     2821 acggcagagc cgcttcgttc ttcctggcgc tcgctgaagc aacacaagac agccgttacc
     2881 gagacgccgc gctctgggca ttggctgcat tcaatgggga cttcagtcgc tacggcttgg
     2941 atgccgcgtg tttcggccag gcgctcggcg agtacgcgat gggtgcgcaa acctaggcag
     3001 caaagatgga gtcgtcgtgc gctatagctc ctccaggtaa aatccggaaa ttatatagtg
     3061 aaaattcgaa cgacaataag ggtgccgtgg aactcacgaa caattgcaat gatcttaacc
     3121 agtactgatc cggctgctga agtactccca cacccgcttg gacacctctt ctccggagac
     3181 ttgcggatag atggtttcga gttcctcggc ggtgaagtaa cgcggtttgc cggcagacat
     3241 cgctcgcagt tggtagtttg ccgcttcctc catccacaag gtcataacgc atacgcggcg
     3301 aacgtcttta tcggctacat tgacgccgtg cccgcgcagt aacatggctc cgtgatcacc
     3361 caaggtgcgc gccacggcat cgcccagttc gcgcgtcctg attagagaga tccgatcgaa
     3421 cacctggaca tcgtttcgaa aaattgcccc cgaagcgtgc agaggccgaa tcgtttcacc
     3481 aagcgagctt aaaaccacgc aactcggcgg atgaacatgg caaatcgcct gaacatcggg
     3541 tctggctttg taaatccgcg catgtatcca aaattcagtg ttgggacggc catcaccgga
     3601 gagttgattt ccttcaacat cgagcaccag cagattgtcg gcacgcacaa gagccgggct
     3661 catccttggc ggaaaaatga aagtatcagt ttccggaata cgcgcactca ggtggccaaa
     3721 cgccgcggcg acaccttgtt gatgtagtat gtggctggcc ttgaccacgt cacttttgat
     3781 cagcgcaaca tccataagtt acctttcgac aatgttgaga aatcgcttcc ggccggcgtg
     3841 tgatcgtcct ctaccttttg atttcatcgc cgatcagtcg caatacctcg ttaaactggc
     3901 gctgcatcgc gatcatctgc tcgcgcatgt tgagaaccac ttcggcgccc tcgaaattca
     3961 cccccatctc ctgaatcaga agattggcaa tgcgaatgcg atccacctga tcgggcggat
     4021 agagcttgcg cttccgctgc agcaccgggc gaatcacgct ctctcgctcg agctgttgga
     4081 tgaaatcctt gtcaacctta caaactttga tcacctctgt gatctcgaaa tatcttcgtc
     4141 ccatcggttg ctctcgttat agtcggacgt ccttgcgcac gtcgtccgcg taggctcgat
     4201 cgattttgtc gattacgtct gcagcgatat tattatccgt gggcacgcga atcatcagct
     4261 gtaaatacag gtctccggca gggctttgcc cgtgtgcgga aactcccttg cccttgaggc
     4321 gtagtcgctg accgctctga gcgcctgcgg gaatttttac cttgagcttg cccatgggtg
     4381 tcggcacttc cactgtcgct ccccgaaccg cttcaccgat tgtgatcggc aactccatcg
     4441 acagatcctt gcccgagcgc gtgagcagcg gatggggacg gattcgcggt acgatataga
     4501 ggtcgcctgg cggcccgcct ctcacaccgg cctccccttt gccgcgcaaa cgaatccgct
     4561 tcgaaggctc ggctccgggt gggatattga cccgaatcgt ctccggccgc agcactcggc
     4621 cggagccacg gcacaccgcg caaggatcgc ccggcagttt tccggttccc atgcagcgcg
     4681 ggcaagtctg gcgaaactgg agcggaccct gcgccaccga tttactgccg ctgccaccgc
     4741 actccgggca ctggctcggg ttgctgccgg gcttagttcc ggagccagtg caggtatcgc
     4801 aggtgaccgg ccgctgcagc gtgatcgaag tttgaaagcc gcggacggcg tcgagaaaat
     4861 cgatatccat agatgattcg atatcttctc cgcgcatcgg tccttgccgg cttcttcgag
     4921 tgcgtgcgcc catgccgccg aacccgccaa agagctcgct caaatcgtcc atatcgaatt
     4981 cgtagccacc gcccgcccca gcagcggact gttgctgcca ttgttggtag ctgcgcgcct
     5041 tgtcagcatc gaaacctgcc gccaagcccg cctcgccgaa ttcatcgtat aatttgcgtt
     5101 tctgctcatc gcctaaaacg tcgtaggcag tcgcgatctc tttgaattta ttctcggcct
     5161 ccttgttccc cggattgatg tcgggatggt gtttgcgggc aagcttgcgg taagccttgc
     5221 ggatatcatc atgcgacgcg ccgcgttcga caccgagaat ttgatagaag ttacgttcag
     5281 ccattgtaca ttagaatatc tttggcactg ccggttgcaa ggggatacga tcttgaattg
     5341 acgatgatgt ttcagacctg tcgaataagc tatgtaaggc gtataagtag aattccaatc
     5401 actaccaggg cgatcccggc ggcagcgcgg ccggacaatt gttcaccaaa caacacagtt
     5461 cccagtatga cagccagcag cgcttcaagt cctaacacca acacatagct cgagccgagc
     5521 tgttcgaatt gcatggcgcg cgcttgtatc aaacaggtgc tcgggcgaaa cttgccagat
     5581 gaaacagcca aacactccct cctccttaca acgttccaac tctctgatgg cattttgatg
     5641 ttgtaaagag agatcatcgg gatgcccacg agccaaagca gcagttgtgc tcagtaggga
     5701 tacctctctt tccgtgattg tctcttgttt agtccgttca aatccgtttg tctaacaaag
     5761 gacctagctc cgtacgatgg ctaggccagt gcaatgacag ttttgaaggc gttcgttcgc
     5821 gtgctgacag agacatggcg aacatgcttc tgtccgaagg cgcaaggctg ccgattgcct
     5881 cttcctacgt cggcgtggca tggcaatatc atcgttttaa cgattgtcgg tagggttcgt
     5941 gcgcaacaag acagggacaa ccgaatacaa ggaacaacgc gggaccggct cacgccgtag
     6001 gatctggatc gcgataggag ttctcgtcgc gcttctcgga atggcggcgg cgtggcgctg
     6061 gacgcctctg gccgatcaga tcgacatcgg caaggttagc gcatgggctg cagcgctgcg
     6121 ccacaatcca tcgcgggtga tgattatt
//
LOCUS       DASUTG010000177         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_324237, whole genome shotgun sequence.
ACCESSION   DASUTG010000177 DASUTG010000000
VERSION     DASUTG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324237"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2327
                     /locus_tag="VF089_03990"
     CDS             <1..2327
                     /locus_tag="VF089_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6173151.1"
                     /translation="LLAVNAIVHWSTVESLLALAVLFLFYLVACARWPIAEEQGIFFN
                     NIYFLVLAGIIVVIGNYLYNQLQFREFVLRFELSQNRRTLEEANQKLIELDRVKSQFF
                     ANVSHELRTPLTLLLTPLQSLIQEYKSELGSPIRDLLLIMQSNGMRLLKLINDLLDLV
                     RLEFGKMEVNSEPVDIDRLVHGVANAVKNTAQDREIEIRVMTDPSVGTMVGDSDKLER
                     ILLNLLFNAIKFTPAGGRIEITVTRAERELLLVISDTGTGISQEQLPFIFDRFWQADT
                     SAQRKNQGLGIGLALVRELVEIQGGAVSVSSELGKGTRFTIRLPYNEIDRPSTYGEKR
                     TNPSENNESPRISSDWNSHAKKLGEIDRLAELYPSIALLRASLRPVGNDIDSKKPRVL
                     IADDEPDMLRYLKSQLSLNFQVIEAVDGRQAIEKASQFLPDVIVCDMMMPEKSGLEVC
                     REIRERTYTKSTPILMLTARADEETKLAALAAGANDFITKPFSTTELRVRLKNLVETF
                     HLQQKLLRQNQLLEATIEQLKDTEVQLVHSEKLASLGRMSAGIIHEINNPLNYAKTGL
                     YVLRNTIESPSTDEKEPSLEVLHDIQEGIDRIDRIVSDLRIFTHPNVTQIERIQLAEL
                     VTSALRLLSHELHGKVSVQNEISNDQTIWANRNQVTQVLVNLLQNAVYAVDKQASSEK
                     PPTIWLRALEDYEHTLVIIRDNGEGISPENLPKIFDPFFTSKDVGEGMGLGLSICYRI
                     MQQHGGSIEVQSERGVYSQFTLRFPRAANGMAAA"
     gene            2338..>3588
                     /locus_tag="VF089_03995"
     CDS             2338..>3588
                     /locus_tag="VF089_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173152.1"
                     /translation="MESAYDYRKCTVLYVDDEEMALKYFARAFENRFPILTALNGADG
                     YRLLEENRERIGLVITDQRMPGEKGVQFLERARQLHPRAIRILTTAYSDLNVAIEAVN
                     SGAIYKYVTKPWDIPQLEVTLQRAVEFFLVQRERDLLLKEKLSVLQRMMITDRVLTLG
                     ILAGRLGHYLRNSLVAVQTFIDLAPEKLLEEKISAEQIRNPSFWKEFYEQAQSQMRRI
                     TELLTDLVSASQSSGSPRLEKVDLKQTVLRSLQRFNGSPLQHGITFVDQIPEGLPLLS
                     VERGQLERLFELLIKDEIMSLPAGSHVFLSATPRRETQEVAIEIRDDGPGLPNEAFLS
                     VFDPFFFRNNNSQEFGINLMACYFIVYHHGGKIEVRNQQGQGVTFTLTFPIKPKNVSP
                     AQEEEAFISKVLMNERFWERLISDQ"
BASE COUNT          956 a          869 c          916 g          847 t
ORIGIN      
        1 tgcttcttgc ggtcaatgca atcgtgcact ggagtacggt cgagtcttta ttggctctgg
       61 cggtcctttt tcttttttac ctggtggctt gtgcccgctg gccgatcgcg gaagagcagg
      121 gaatattttt taataatatc tatttcttgg tgcttgcagg aattatcgtt gtcataggca
      181 attatcttta caatcagctc caatttcgcg aattcgtgct gcgcttcgag ttgagccaaa
      241 acagacgaac actggaagaa gccaatcaga agcttattga gttagaccga gtcaagagcc
      301 aatttttcgc taatgtgagc catgagttgc gaactcctct cactttgttg ttgacgccgc
      361 tgcaaagtct aatccaggag tataaatcgg agttgggatc acccatccgt gatctgctgc
      421 tgatcatgca aagcaacggc atgcgactgt tgaaactgat caacgattta ttggacctag
      481 ttaggctgga gttcggcaag atggaggtga attcagagcc ggtggacatc gatcgtttgg
      541 tgcacggtgt tgcgaatgca gtaaaaaata ccgcccaaga tcgtgagatc gagattaggg
      601 taatgaccga ccccagtgtt ggcacgatgg tcggcgacag cgataaactc gaaaggattt
      661 tgctcaatct gctgtttaat gcaatcaagt ttacgcctgc cggcgggagg attgagatca
      721 ccgtcacgcg cgccgaacgt gaactgctgc tggtaatttc cgacactggc actggcatca
      781 gccaggaaca gctgccgttt atctttgacc gcttctggca agcggatacg tccgcccaac
      841 ggaagaacca aggattaggt ataggactcg cactggtgcg agaacttgtg gaaatccaag
      901 gcggcgcagt aagtgtctct agcgagcttg gcaaaggaac ccggtttacg atccgattgc
      961 catataacga gatagatcgc ccttccacct atggcgaaaa gcgaactaac ccctcagaga
     1021 acaacgagtc tccgcgaatt tcatcagact ggaacagcca tgcgaaaaag ctgggcgaaa
     1081 ttgatcgact agccgagctt tacccatcca tagctcttct aagagcaagc cttcgaccgg
     1141 tgggaaacga tattgacagc aagaaacctc gggtcctgat tgctgatgat gaaccggaca
     1201 tgctgcggta tttaaaatca caactgagcc ttaatttcca agtgatcgag gccgtcgatg
     1261 gccgccaggc cattgaaaaa gcgagccaat ttctaccaga tgtgatcgtc tgcgacatga
     1321 tgatgcctga aaagagtggg cttgaggttt gccgcgagat cagggaacgc acttacacga
     1381 agtcgactcc cattctgatg ctcacggccc gggctgacga ggaaactaag cttgcggcat
     1441 tggcggccgg cgcaaacgat tttattacca agccgttttc cacgaccgag ctacgggtcc
     1501 gtttgaagaa cttggtcgaa acatttcatc tacagcagaa attgctgcgg caaaatcaac
     1561 ttctcgaagc gacgattgag caactaaaag acaccgaggt gcaactggtt cattcagaga
     1621 agctagcctc gcttggtcgg atgagcgccg gtatcatcca tgaaatcaac aacccgctca
     1681 actacgcaaa aaccgggctc tatgtacttc gcaacacaat cgagtcgcca agtaccgatg
     1741 aaaaggagcc ttcattagag gtgctccacg atattcaaga agggatcgat cgaatcgata
     1801 ggatcgtatc agatttgcgc attttcactc accccaacgt gacgcaaatc gaaaggattc
     1861 aactcgccga gcttgtgaca tctgcattac gtttgctaag ccatgaatta cacggcaagg
     1921 tctcagttca aaacgagatc tctaatgacc agacgatctg ggctaatcgc aaccaagtta
     1981 cacaagtgct ggtaaacctg ctgcaaaacg ctgtttatgc cgtagacaaa caagcgagca
     2041 gtgagaagcc gccgacaatt tggctgagag cgctcgaaga ctatgagcat actctagtta
     2101 ttatccgtga caacggcgag ggaatctcac ctgagaacct tccaaaaatt ttcgatccat
     2161 tttttaccag taaggacgtc ggagaaggta tgggacttgg gctgagcatc tgctatcgta
     2221 tcatgcagca gcacggcgga tccatcgaag tccagagcga acgaggtgtt tactcgcagt
     2281 ttactttgcg ctttccacgt gcggcaaacg gcatggcggc tgcctgaggg agagtttatg
     2341 gaatcggcct acgattatcg gaagtgtacg gtgctttatg tcgatgatga ggagatggct
     2401 ttaaaatact ttgcccgggc tttcgagaac aggtttccga tcctcactgc gctcaatggc
     2461 gctgatggtt acaggcttct ggaggaaaat cgcgagcgga ttggccttgt gattaccgat
     2521 caacgaatgc ccggggaaaa aggagtgcag ttcttagaac gggctagaca gttgcacccc
     2581 agggcaattc gtatcttgac cacggcttac tccgacctga atgtagctat cgaagcggtt
     2641 aactctggcg ctatctataa atatgtaacg aaaccctggg acatacctca gcttgaagta
     2701 accctccaac gcgctgtcga gtttttcttg gtgcagcggg aacgcgatct tcttctaaaa
     2761 gagaaactct cagtcctaca gcgaatgatg attaccgacc gggtgctcac tttaggaatc
     2821 ctagccggtc ggctggggca ctatttgcgc aactctctgg tagcagtcca gacttttatt
     2881 gatctcgcgc cggaaaaact cctggaggaa aagattagcg cggaacagat tcgcaacccg
     2941 agtttctgga aggaattcta tgaacaagcc cagagccaga tgcggcgcat aaccgagcta
     3001 ttgaccgatc tggtaagcgc aagccaaagt agcggctcac cacgtcttga aaaagtggac
     3061 ctgaaacaaa cggtgctacg aagcttgcag cgattcaacg gcagcccgct gcaacatggc
     3121 atcacattcg tggatcaaat cccggaaggg ctgccgctat tgtccgtcga acgaggacag
     3181 cttgaacgtc ttttcgaact attgataaag gacgaaatca tgagcttacc cgcgggcagt
     3241 catgtttttc ttagcgcgac tcctcgtagg gaaactcagg aggtagccat tgagattaga
     3301 gatgatggtc ctggcctgcc caacgaagcc tttctttccg tgttcgaccc ttttttcttt
     3361 cgcaataaca attcccaaga attcggcatc aatttgatgg catgctattt catcgtgtac
     3421 catcacggcg gaaagatcga ggtccggaac cagcaaggtc aaggcgtcac gttcacgctg
     3481 acgttcccaa taaaacctaa aaacgtctca cccgcgcaag aggaagaggc atttatatcc
     3541 aaagtactga tgaacgagcg attttgggaa cgtctgattt ccgatcaa
//
LOCUS       DASUTG010000178         3645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1807343, whole genome shotgun sequence.
ACCESSION   DASUTG010000178 DASUTG010000000
VERSION     DASUTG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807343"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1937
                     /gene="bamD"
                     /locus_tag="VF089_04000"
     CDS             72..1937
                     /gene="bamD"
                     /locus_tag="VF089_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX6173153.1"
                     /translation="MKNKFPLALLALAIGLPGCSSLSMPSVPWFKSAAPADPTAEALF
                     AEGSRYFNEKKYARAIDVLQKLKSGHPFSPLLTETELKVADAYYLNQQYPEAITAFKE
                     FQSLHPTNENISFVILRLGQAHLNQFTSVDRDQKNTEIAKGYFETVITNHPKSPHARE
                     AAEKLAKCIQYLSEHEFNVAIFYHQQEKYPAARDRFEEIVRKYKGTPTAIKSLYYLGE
                     SYRKEKNWVKASLAYEALVQHYPETKFAVDARTQLAQLDKEKQDPLALLLMRDRRPGA
                     APAPEAREEPALSKLKNLNLVAKKDLVYEEPGDEKGVLRRVADKLNPFSSADEPKESA
                     TELLVNRKEAESKESSGILASLWPFGRQNSSSNSKPSQNTGLVGHIDDSLKQKGINNT
                     AGQASLTPPVADLPKNDEAPPQKMDTGVLLGSIDSTLKKSGKTPELPPAPEAAAALKD
                     PPAIQTPAVKTATQPETPVSSGLLSNIDQKLKSQGVEPSRFESPPSRAEVTESTVKNT
                     PPVKVELEPKVSAGKGPLFLAPAELPSKPTSDRADDANTTDAKPEVAETDQPASKEAP
                     RNLVRGPIQPQPTNRTTTAAEQKKPAPGEEENKGLFDYLRDDVENVSKILNPFRW"
     gene            complement(2301..>3645)
                     /locus_tag="VF089_04005"
     CDS             complement(2301..>3645)
                     /locus_tag="VF089_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6173154.1"
                     /translation="SDRPLSADELIRDLRPKLARVPGIRVSLQNPPVIRVGGRLSRSL
                     YQFTLQSPDATELYRHAASFEEKLRALPDMRDVGSDLQLKNPQLQVVIDRDKMATMGI
                     TAQGISPRQVEEAFWNAYATRQITTINTPNNQYQVIVELDPKFQNDPSALSQLYIRST
                     TGQTATAQPMVPFGALASLIYSAGPLSVNHSGQLPSVTFSFNLREGVALGDAVETINQ
                     LARENLPGTISTTFQGTAQEFQSSIKSLWLLLALAVFVVYLVLGILYESFIHPLTILS
                     GLPSAGLGALIALYLLQMDLSIYAFVGLIMLIGIVKKNAIMMIDFALERQRNDGKSPA
                     QAIYEGALVRFRPIMMTTMAAIVGAIPIAIGWGAGAEARQPLGVAVVGGLLVSQILTL
                     YITPVMYLYLERGQNFLVSLPSKVRGAGKGSPADAATTAMGAKLGVENPGQHNIT"
BASE COUNT          958 a         1004 c          926 g          757 t
ORIGIN      
        1 aacagcacaa taagcttgac tctgcctccg gtgggcatat tatggaaaaa cctcacatca
       61 gtaggtgaaa aatgaaaaat aagtttccgc tcgctctcct tgcgcttgcc attggactgc
      121 caggttgctc gtcgctttcg atgccatccg tcccctggtt caaatccgca gctccagcag
      181 atccgaccgc cgaggcttta tttgccgaag gcagccgcta tttcaatgaa aagaaatacg
      241 cgcgcgcaat agacgttttg caaaagttaa aatcgggaca tccgttcagc cctctactca
      301 ccgagaccga gttgaaagtt gccgatgctt actacctaaa tcagcagtat cccgaggcga
      361 tcaccgcatt caaagagttt cagagtcttc acccgaccaa cgaaaatatc tcttttgtca
      421 tcttgaggct tggacaagcc cacctgaacc aattcacgtc ggtcgaccga gaccagaaaa
      481 atactgagat cgccaagggc tatttcgaaa ccgtcatcac caatcatcca aagtcgccgc
      541 acgcccgcga ggccgcagag aagctggcca aatgcatcca gtacctttcg gagcacgaat
      601 tcaatgtcgc gattttctat catcagcagg aaaaatatcc tgccgcgaga gatcgtttcg
      661 aagaaattgt ccgcaagtac aagggaaccc cgaccgcgat taaaagcctt tactacctgg
      721 gagaaagtta cagaaaagaa aaaaactggg taaaagcttc tctcgcttac gaagcgctcg
      781 tccagcatta tccggaaacc aaattcgccg tcgacgcgag aactcagctg gcgcaactcg
      841 ataaagagaa acaggatcca ctggcactgc tgttgatgcg agatcgtcgg ccgggcgccg
      901 ctccggcgcc ggaggcgaga gaggagccgg cgcttagtaa actgaagaac ctgaatttag
      961 tagcaaaaaa agacctggtg tatgaagaac ccggagacga gaaaggagtt ctgcgccgtg
     1021 tggcagacaa gttaaatccc ttttcctcag ccgatgagcc aaaagaaagc gcaactgaac
     1081 tgctggtcaa ccgcaaagaa gccgagagca aggagtccag cggtattctc gcatctctgt
     1141 ggccattcgg caggcagaac tcgagctcga acagcaagcc cagccagaac accgggctcg
     1201 tgggtcacat cgacgactcc ttaaagcaaa agggtattaa taataccgcc gggcaggcaa
     1261 gcttgactcc gcctgtggca gatctaccga agaacgacga ggcgcctccg caaaagatgg
     1321 atacaggtgt gctcctgggt tcgatcgact caacccttaa gaagagcggt aagacccccg
     1381 aactgccgcc ggcgccggag gccgcagcgg cacttaaaga tccgcctgcg attcaaaccc
     1441 ctgccgtcaa gacagccact cagccagaaa ccccggtcag cagcggactc ttgagtaata
     1501 tcgatcaaaa gctaaagtca cagggagtgg aaccgtcgcg attcgaatcg ccgccttccc
     1561 gcgctgaggt cactgaaagc accgttaaga atactcctcc cgtgaaggtc gaattggagc
     1621 caaaagtaag cgcggggaaa gggccgttgt ttctagcgcc agcggagctt ccgagcaaac
     1681 ccacaagcga tcgtgcggat gatgcgaata ctaccgatgc caagcccgag gttgcggaaa
     1741 cagatcagcc tgccagcaag gaagctcccc gtaatctcgt ccgcggaccg attcaaccgc
     1801 agcccacaaa tcgaacaaca accgccgctg aacagaaaaa accggcccca ggcgaagaag
     1861 aaaacaaagg attgtttgac tacctgcggg acgatgtcga gaacgttagt aaaatcttga
     1921 atccgtttcg ctggtaagtt tgagtcaaag ttccgagtta cgcgctcaca tccttgtgcc
     1981 ataaagaccc tatggtctgg aggattcacg cggctatgcg cttaacaccg cgcgttgttg
     2041 atacgcggtg tgttcttcat tcttcttcat agaaccgccg ttctcccctt tagccaaaca
     2101 gaccgggtcg aaacttcgct acctgcgcaa gtcaggccaa tatcatcctc atgcacgaat
     2161 agtatcggcg ggctgcaatc aactcatcta tgatggctct cgcagtggca cagagctagc
     2221 gctcatgggc gctgcaggcc gtgaattgcg ggcggggtta tgatgccgcc attgtgtcat
     2281 ctcgacacgt aaggaacggg tcaggtgatg ttatgctgtc ctggattttc cactccgagt
     2341 ttagcgccca ttgccgtcgt tgcagcatcg gctggagaac ctttaccagc tccgcgcact
     2401 ttcgagggaa ggctaaccaa aaaattctgg cctctttcga gataaagata catcaccggc
     2461 gtgatgtaga gcgtcaggat ttgcgatacc agcagaccgc caactacggc gacacccaag
     2521 ggctgtctgg cctcagcgcc ggcgccccag cctattgcaa tgggtatcgc gccaacgatt
     2581 gcagccatgg ttgtcatcat gatcggccga aagcgcacta acgctccttc ataaatagct
     2641 tgggctgggc ttttgccgtc gttgcgttgc cgctcgagag caaagtcgat catcataatg
     2701 gcatttttct ttacgatacc aatcaacatg atcaaaccga cgaaagcata aatgctcagg
     2761 tccatctgca gcagatagag cgcgatcaat gcacccaaac cggccgatgg caatcccgaa
     2821 agaattgtca gcggatggat aaagctttcg tacaggatgc ctaaaaccaa gtaaaccacg
     2881 aagacagcca gagctaagag cagccagaga ctcttgattg atgattgaaa ctcctgggcg
     2941 gtgccttgga acgtcgtgct gatggttcca ggtaaattct cgcgggctaa ctggttgata
     3001 gtctccaccg cgtctcccag agcaactcct tctctcagat tgaaagaaaa cgtcaccgac
     3061 ggaagctgcc ccgaatgatt caccgatagc gggcccgcgg aataaataag actcgccaac
     3121 gctccgaacg gtaccatcgg ctgggctgta gccgtctgac ctgtcgtcga tcggatatac
     3181 aactgcgaca gcgctgacgg atcattctgg aatttggggt ccaattcgac gatcacctga
     3241 tattgattat ttggcgtgtt gatagtcgta atctgtctcg tggcataggc gttccaaaaa
     3301 gcctcttcaa cttgtcgagg agaaatgcct tgcgccgtaa tccccatcgt agccattttg
     3361 tcccgatcaa tcacaacctg caattgcggg tttttgagct gcaaatcgct gcccacatcg
     3421 cgcatatcgg gcaatgctct aagcttctct tcaaaggacg ctgcatgacg atagagttca
     3481 gtggcatcgg gactttgcaa cgtgaattga tacaagcttc ggcttagccg gcctccgact
     3541 ctaatcaccg gcgggttctg caaggacacc ctgattccgg ggactcgtgc cagctttggg
     3601 cgtaaatccc ggatcagctc atcggcactg agagggcggt ccgag
//
LOCUS       DASUTG010000179         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1066274, whole genome shotgun sequence.
ACCESSION   DASUTG010000179 DASUTG010000000
VERSION     DASUTG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066274"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VF089_04010"
     CDS             complement(<1..598)
                     /locus_tag="VF089_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173155.1"
                     /translation="MSKAATAQKEQLKAAPLDPSVRALGVVSLLNDFSSEVAVRTLPL
                     FLANVLGVRIGIIGLIEGIAESTATLLKLVSGYLSDRFHRKKALALWGYGFSNLTKPL
                     LYFANSWGLVLAVRFLDRVGKGVRSAPRDALIADLTPPGLRGRAFGFNKAMDKTGGFL
                     GLVVAAGVLYLTQTDTVALTRESYEWLVLLAVVPGLAAV"
     gene            complement(672..1298)
                     /locus_tag="VF089_04015"
     CDS             complement(672..1298)
                     /locus_tag="VF089_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6173156.1"
                     /translation="MESPRAHISRISNNDAALRRAVQIVALLNLAYFGVEFGVALAIG
                     SVSLFADSIDFLEDAAVNGLILIALNWNLHRRSLVGMVLAAILLAPGLATLWTAWEKI
                     VAPLPPAPIPLSLAGGGALAVNLFCAGLLARFRTHSGSLTRAAFLSARNDALANVAIV
                     AAGLVTIVTLSAWPDLLVGLGIFFMNLDAAREVYTTARRERSLAVGQP"
     gene            complement(1354..1683)
                     /locus_tag="VF089_04020"
     CDS             complement(1354..1683)
                     /locus_tag="VF089_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX6173157.1"
                     /translation="MFAISFSLSLFVLAGLCEIAGGWMVWQSLRESRPWTWGLLGAVL
                     LVLYGVVPTFQTAHFGRVYAVYGGFFIVLSLLWGWVLDGNRPDHLDLIGGAVSLVGVA
                     IMMYGPR"
     gene            complement(1709..1996)
                     /locus_tag="VF089_04025"
                     /pseudo
     CDS             complement(1709..1996)
                     /locus_tag="VF089_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            complement(2009..2206)
                     /locus_tag="VF089_04030"
     CDS             complement(2009..2206)
                     /locus_tag="VF089_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173158.1"
                     /translation="MRTKLLVSFILTVGILMPHFGDRATAAQARNDPAPSFTLKLLSG
                     KDFDFSELKGKPAVLKFVSSY"
     gene            complement(2245..2451)
                     /locus_tag="VF089_04035"
     CDS             complement(2245..2451)
                     /locus_tag="VF089_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6173159.1"
                     /translation="MFCDKAKEFLSQRDVVFEEKDITKDPSAVDELEKLGVMTTPVIV
                     IDGETVVGFNQKRLEELVERPELP"
BASE COUNT          662 a          751 c          652 g          528 t
ORIGIN      
        1 cgacagcggc cagaccggga acgacggcga gcagcaccag ccattcgtag ctttcccgcg
       61 tcaacgcgac cgtgtctgtt tgcgtcaggt acaacacgcc ggcggccacg accaggccga
      121 ggaacccgcc ggtcttgtcc atcgccttgt tgaagccgaa ggcacggccg cgcagccccg
      181 gcggcgtgag atccgcgatc aaagcgtcgc gcggggccga ccggacgccc ttgccgactc
      241 ggtccaggaa tcgcaccgcg agcaccaggc cccacgaatt cgcgaaatag agcagcggct
      301 tggtcaagtt tgaaaacccg tacccccaca atgccagcgc ctttttccgg tggaaccgat
      361 cggaaagata tcccgagaca agtttgagca gggtcgccgt gctttccgcg atgccttcga
      421 tcagaccgat aatgccgatc cttacgccca agacgttggc gagaaacagc ggcaacgtgc
      481 gcacagcaac ctcgctggaa aagtcgttca gcaacgaaac cactccgagg gcgcggacgg
      541 agggatcgag cggcgcagcc ttgagctgtt ccttctgtgc ggttgcggct tttgacatgg
      601 ctccgctata tcgctcacga gtccttatgc gaaggggcta gcagtgctca tggtagaggt
      661 aatgagaaaa gttagggttg gcccacagca agcgatctct cgcggcgggc ggtagtgtac
      721 acttcacgcg ccgcgtccag gttcataaag aaaattccca agccgacaag aaggtcgggc
      781 caagccgaca gggttactat cgtcactaga ccggctgcaa cgatggcaac attcgcaagt
      841 gcatcgttgc gggccgaaag aaatgcagct ctcgtcaagc taccgctgtg ggtacgaaaa
      901 cgagcgagaa ggccagcgca gaacaggttt accgccagtg ccccaccgcc agccaacgac
      961 aatggaatcg gagccggtgg aagcggcgca acaatcttct cccaagctgt ccacagtgtt
     1021 gctaaacccg gagcgagcaa aatagccgcg agaaccatac caaccaatga acgtctatga
     1081 agattccagt ttagagcgat gagaatgaga ccgttgaccg ccgcatcctc gaggaagtct
     1141 atggagtcgg cgaagagtga gacggagcca atcgccagag ccaccccgaa ttcaactccg
     1201 aagtaagcaa ggttcaacaa cgcaacgatc tgcacagccc ggcgcagagc agcatcatta
     1261 ttagaaatcc ttgagatgtg cgcacgcgga ctttccactt ttgccttcag gttccgcttc
     1321 acgccttgag cagtatgccc gatcgggttt tggttatctc ggcccgtaca tcatgatagc
     1381 cacccctacc aagctcaccg caccgccgat caaatccaag tgatccggcc tatttccatc
     1441 taagacccag ccccaaagga gagacaacac gataaaaaat ccaccgtata cggcatagac
     1501 acgaccgaag tgagctgttt ggaaagttgg gactacaccg tagagaacca agagcacggc
     1561 gccaagtaaa ccccaggtcc acggccgaga ctccctcagc gactgccaga ccatccatcc
     1621 tccggcaatc tcgcataaac cggccaagac aaataagctc agcgaaaaac ttatcgcgaa
     1681 tatcgacact ggccctccgt gcgctgcact atgcagaagt ttctctatct ttttccgcaa
     1741 tgccttctcg ctttgaggtc cggtccttcg ttcgatgacg tagccgtctt tggaaaccaa
     1801 aaccgtgaac ggtcccgata aatttgtaag tgtcggcgat cttcctgccg tcgtcgagac
     1861 cgacaggaaa gctaatcccg taggtttgaa cgaacttctg aacctcagcc tccctgtctt
     1921 tgacgaaaat accgacaaac tgaacatctt tgttcttgaa ctcacgatac actttttcca
     1981 cggcaggagc ctcctgccgg cactccggtc aataggaaga cacaaatttt agaactgccg
     2041 gctttccttt aagctccgaa aagtcgaaat cttttccgct caagagctta agcgtgaagg
     2101 aaggagcggg atcgtttctg gcctgtgccg ccgtcgccct atcgccgaaa tgaggcatca
     2161 agatgccgac cgtaaggata aaagaaacca acaattttgt tctcatgata tttcttcctg
     2221 gttatcttca aaaaccctga cagatcaagg aagttccggt cgctcaacaa gttcttcaag
     2281 gcgcttctga ttaaaaccaa caaccgtctc gccatcgatg acgattactg gagtcgtcat
     2341 cacgcctaat ttttcaagct cgtcgactgc ggatggatct ttggttatgt ccttctcttc
     2401 aaaaacaacg tcccgttgcg agagaaactc cttcgccttg tcgcagaaca gtcagccagg
     2461 ctggctataa acagtaattg ttttgtccat aggtttcccc ttctcgttcc tgatctacaa
     2521 acgtacaatt acgcgatacg agcgaacttg aatgccgccg agttcgaaaa ggatcggata
     2581 catgtggttt aaa
//
LOCUS       DASUTG010000180         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2104634, whole genome shotgun sequence.
ACCESSION   DASUTG010000180 DASUTG010000000
VERSION     DASUTG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104634"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF089_04040"
     CDS             <1..530
                     /locus_tag="VF089_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6173160.1"
                     /translation="ATVGALGSSSGGHVMELCAMRPHDPRYSAHSLPEAADIDATLAY
                     LATRSPVSDPFTRFKHAEKMGREEMVRFSQIYFNPWETIHEGNPQEILDRGEAVTLPP
                     LLIMQGELDDNVLPSAQEKFAASYRAAGGDCELEIFAGCEHRWVAEPGPQTDRAAEMV
                     KDFIARQLSSFRRAA"
     gene            complement(992..2824)
                     /locus_tag="VF089_04045"
     CDS             complement(992..2824)
                     /locus_tag="VF089_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6173161.1"
                     /translation="MELGGRKSDLSRRDLVTTTATADDMDESFGPGTLGARYGEVPQS
                     WDFEADVVVIGSGAAGLPAAIRAVESGASVIVVEANYDVGGHAILSGGNVPLGGGTSA
                     QRKFGIEDSPDVVFSDLTDWSIVQPNGWPDYRYNDREVMRAFADHCAPTYEFLVANGV
                     EFKDIPPDNQGAHTTGNSAPRENHTFWKKGAGPESPNARPGTGLIRPLEASAREKGVK
                     FLLNYKMTTLIRETQGARRVVGMTAYYRPRIMPGESTPLKSFRADGNIDSSKPNVNIR
                     AKKGIIVATGGMTSNLHFRRMFDPRLTDVLQVAGEPYSYQDASGELAGMAIGASLWGF
                     ANQILENGDNIRTQRAIGTKFNYMTWELESPIFPVVKAQGLSVKNWHDLILVNQVGKR
                     FYDETKGDYPHGNMCNDICPYTPNDYRNNEIIEFHPTKYNFFNAAVAMNEYSQPPDYS
                     AGPVWAIFDAEAVKRNSWNVAPPYVDPDGYFFSANTLAELARAIKNEYQAKPMNGATL
                     EATVARYNSFVDAGEDKDFGKPAPKFKIQTPPFYAAWGTPLVHDSRTGLRINAKCQVI
                     DLNGQLIPGLYCAGESAGGFNQHGMGRCATQGFIAGKNAAVEKP"
     gene            complement(3082..3513)
                     /locus_tag="VF089_04050"
     CDS             complement(3082..3513)
                     /locus_tag="VF089_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6173162.1"
                     /translation="MDWLFQTTDSLSYVFLRFGLAVTFFFHGTQHAFGWHGGKGIKDQ
                     VANWRNKYHIPVAIGMFGTFVELFTVVSMALGFLVRPAAFFLAIFIATAMFKSHWGHG
                     FFLQQRGGGSGIEYTLALLLMTLSVLFGGAGAFSIDRMISR"
BASE COUNT          794 a         1093 c          943 g          793 t
ORIGIN      
        1 cggctacggt cggcgcgctc ggcagctcca gcggcggcca tgtgatggag ctttgcgcca
       61 tgcgcccgca tgatccgcgc tatagcgcgc attcactgcc cgaagcagcc gatatcgatg
      121 ccacgttagc ctatttagca acacgatccc cggtcagtga tccctttact cggtttaagc
      181 acgccgagaa aatgggtcgc gaggagatgg tgcgattcag ccaaatttat ttcaatccat
      241 gggaaaccat ccacgaaggc aacccacaag aaattctcga tcgcggcgag gcggtgaccc
      301 tgccgccgct gctgatcatg caaggtgaat tggacgacaa cgttctgcca tcggctcagg
      361 aaaagttcgc cgcatcctac cgcgcggccg gcggcgattg tgaattggaa attttcgccg
      421 gctgcgaaca tcggtgggtc gccgaacccg gaccgcagac cgatcgagcg gctgagatgg
      481 tgaaggattt catcgctcgg cagttgagct cgttccgtcg agctgcttag cggcaagctt
      541 aatctgccgt tttggctgcg tcgactagga ggttcgaccc agtcggcacc tccatgtggg
      601 gactcggatt ccagactctt agaacaacgc gcggagcaat ccgcctacgg ctcggaacgg
      661 taacgcaatc acctctccaa tcacgcttac ggcaccacta accagaccgg agtcttcgca
      721 gatggatcga tctgtagtgg ttctctcgac cactatcgga tctgccgcgc gccgatcttc
      781 atactgcacc gtttcgttct ctgatgggct tgatcacgat gtgcaagaag tcgaaggaca
      841 atcggcgaag caacaacgga acagatgtgt cagaatccga gaggtcgtgc gtacacaaca
      901 tagcacgtga cgcggttcac ccggttaatc ttcacaccag cgaaaccgca agccaagaac
      961 atcttgctct cgtgttgcgc gagagctgtc gctacggctt ctccaccgcc gcattcttgc
     1021 cggcaataaa cccctgagtc gcgcagcgcc ccatgccatg ctgattgaaa ccgcctgctg
     1081 actcgccagc gcaataaagt cccgggatca gctgtccgtt taagtcgatc acctgacatt
     1141 tcgcattgat ccttaagccc gtccggctat cgtgaaccag cggggtcccc cacgcggcgt
     1201 aaaacggcgg cgtctgaatc ttgaacttgg gcgccggctt gccgaaatct ttatcttctc
     1261 cggcatcgac gaacgagttg taccgggcca ccgtcgcttc caaggtcgcg ccattcatcg
     1321 gcttggcctg gtactcgttc ttaatggcgc gcgccaattc cgcaagcgtg ttggcgctga
     1381 aaaaatagcc gtcgggatcg acatacggcg gcgccacgtt ccaactatta cgcttgaccg
     1441 cctcggcatc aaagatcgcc cacaccggcc cggcggaata atccggcggc tgcgaatatt
     1501 cattcatcgc cacggcggcg ttgaaaaagt tatatttcgt cggatgaaat tcgatgatct
     1561 cattgttgcg ataatcgttc ggcgtgtaag ggcaaatgtc attgcacata ttgccgtgcg
     1621 ggtagtcgcc cttggtctcg tcgtaaaatc tcttacccac ctgattgacc aagattaaat
     1681 cgtgccagtt tttgacgctc aacccctggg ccttaacaac cgggaaaatc gggctctcaa
     1741 gctcccaggt catgtagttg aatttggtgc cgatggcgcg ttgggtgcgg atgttgtcgc
     1801 cgttttccaa aatctgattg gcaaagcccc atagcgatgc gccaatggcc atgcccgcca
     1861 gctcgccgct ggcatcctga taggagtaag gctctcccgc aacctgcagt acatcggtaa
     1921 gacgcgggtc gaacatgcgg cgaaaatgca gattactggt cattccgccg gtggcgacga
     1981 tgattccctt cttggctcga atgttcacgt taggcttgct gctgtcgata ttgccgtcgg
     2041 cgcggaagct tttcagcggc gtcgactcac ccggcataat ccgcggtctg tagtaggccg
     2101 tcatgccgac tacacgacgc gcgccttgcg tctcacggat caaggttgtc atcttgtaat
     2161 tcaataggaa cttcacgccc ttctcgcgcg cgcttgcctc cagcggccgg ataagaccgg
     2221 ttcccgggcg ggcattgggg ctctccggcc ccgcgccttt tttccagaag gtatgatttt
     2281 cccgcggcgc agagttgccg gtggtgtgcg caccctgatt atccggcggg atgtccttga
     2341 actctacgcc gttggcgaca agaaactcat aagttggcgc gcagtgatca gcgaacgcgc
     2401 gcatcacctc acgatcgtta tagcggtaat cgggccaacc attcggctgc acgatcgacc
     2461 aatccgtcaa atcgctaaaa accacatcgg gagaatcctc gatgccgaac tttctttgcg
     2521 cgctggtgcc gccgccgagc gggacattgc cgccgctcaa aatggcgtgg ccgccgacgt
     2581 cgtaattcgc ttccaccaca atcactgaag cgcccgactc gaccgcgcga atagccgcgg
     2641 gaagaccggc cgcgccggag ccgatcacga ccacgtcggc ttcaaaatcc cagctctgcg
     2701 gcacctcccc atatcgggcg cccaacgtac cgggtccgaa gctctcatcc atgtcatctg
     2761 cggttgccgt ggttgtcacc aaatcacgtc gtgagagatc gctctttctt ccgcctagtt
     2821 ccatccgtaa ctctcctttc acacccgtct ggcagactgc agaaaagacc tatatccttc
     2881 gttgcctcaa atcgcctcgc tgcgacatac tgtgagtacg cctacgctcg tcgatttttc
     2941 gcgcgccgcg cctctgaatc tttttggcca gcctgcaacc aaatttcaag caaccggcta
     3001 gctacacgac tttatcttcg atgcatctaa cgtaagacaa tcatcgatct tcgtcccaag
     3061 agccgtatcc gccgcgggac tctagcggct aatcatccgg tcaatcgaga acgcgccggc
     3121 gccgccaaaa agtaccgaca atgtcatgag cagcagcgca agggtatatt cgataccgct
     3181 tcctcccccc ctttgctgca gaaaaaaacc gtgcccccag tggctcttga acatagccgt
     3241 agcgataaag atcgccagaa aaaaggctgc cgggcggacc aagaaaccaa gcgccatgga
     3301 gactacggta aaaagttcga cgaaggtgcc gaacatgccg atggcaacag gaatgtgata
     3361 cttgttgcgc cagttggcaa cctgatcttt gatgcctttg ccgccatgcc agccaaacgc
     3421 atgctgagtt ccgtggaaaa aaaacgtcac cgccaggcca aaacgtaaaa acacataaga
     3481 caacgagtca gtcgtttgaa ataaccagtc cactcttcct cccgatatct gttgtgacgt
     3541 agatcgagcg tgcatcgcag ttgcttatac gatgcgagcc cgattaggat tatccgtctc
     3601 tcttatttca attttgtccc tct
//
LOCUS       DASUTG010000181         7619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_29664, whole genome shotgun sequence.
ACCESSION   DASUTG010000181 DASUTG010000000
VERSION     DASUTG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29664"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..944)
                     /gene="cysK"
                     /locus_tag="VF089_04055"
     CDS             complement(3..944)
                     /gene="cysK"
                     /locus_tag="VF089_04055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX6173163.1"
                     /translation="MTLNGAGSILDLIGSTPVLKLKRLPGKDDAEVWAKLEAFNPGGS
                     VKDRICCSMIEAAEREGKLKPSSTIVEPTSGNTGIGLALVAAVKGYRLVLTMPDTMSE
                     ERRSLLAAYGARLILTPDTKGMGGAIHKAEELVQENSDYFMPQQFNNPANPEAHRRTT
                     AMELLKQFNRIDAFVAGVGTGGTITGVGEVLKDRFKAVRICAVEPASSPVISGGEPGY
                     HKIQGLGAGFIPAVLNRQVIDEVIPVSDTDAINYTRRLASEEGLLVGISSGAACCAAL
                     RVAKTLGKGQIVVTVFADKGEHYLSTELFGENRWTQN"
     gene            1050..2081
                     /locus_tag="VF089_04060"
     CDS             1050..2081
                     /locus_tag="VF089_04060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX6173164.1"
                     /translation="MSNRRALVVFPGALGDFVCFLPAFEVLAERASIDLLARTEFSEL
                     VPARVNVASLERFEIRHLFVSGGGSDEKVRSFFETYSCTYSWMGSGDLTFKQELQAAT
                     QGRIRIFQFYPRNSLMHQADYYLSCLRANSAPGMPHISLMPDAKQWAEAYWAKHSLES
                     SPVLAIAPGSGAREKNWPVISFEAIARWWRERVGGSIITILGPVEDERGGYESLCNYG
                     LAIRGINLGHLTATIARCSLYVGNDSGMSHIAAALGVPTATVFGPSSINQWAPRGNTV
                     LVLRHEVPCSPCPVSAMKNCDHRDCLAALTPEEVIRKLESWSPLVTLTSWGAGIRVLP
                     KISPELTGG"
     gene            2215..2655
                     /locus_tag="VF089_04065"
     CDS             2215..2655
                     /locus_tag="VF089_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX6173165.1"
                     /translation="MAGEEEKKEGKGFTVQDRRRFSETGDSRDDAPSSADAGESVQRK
                     PTEDATSRSPGKAEEQESALPEINFSTFIISLSTQALMHLGEIANPLTGKVEPDRPVA
                     KQMIDIIGMLREKTRGNLNSGEDRLIEDILFDLRMKYVEAVKKK"
     gene            2665..4164
                     /locus_tag="VF089_04070"
     CDS             2665..4164
                     /locus_tag="VF089_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6173166.1"
                     /translation="MDIKRVWSSSFTFKGVAVAAIASLLLGLAISGSFDWLAPSRAVN
                     MGDGASSDVRIANQLPDFVALSKKLRPVVVNISSTQVSEARSQQGPQEFGSPFGEEDP
                     FNDFWRRFFGGPIPRGPQRQRSLGSGFIIDGDGSILTNNHVVENAQKIVVKLADEQEY
                     EAKVIGRDPKTDIAVIKIEAKTKLTPANFGDSDGLEVGEWVMAIGNPFGLDSTVTSGI
                     VSAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEVIGINTAIFSRTGGNIGIGF
                     AIPVNLVKELLPQLRGKGKVTRGYLGVLIQKVTPEIAESLGMEKSQGALVANVSKDGP
                     ADKSGVKVGDVIVEFDGKEIKDSGELPIIVARTPVDKKVKMKVLRDKSEVTLNVAVGE
                     LKDEEIVASAPEKGEMGLTVQRLTPQIAESLGLEKTDGIVVSAVEPGSAADEAGIRRG
                     DVIVEIDRKPIRNLEEYKKSVASVRKGKGVLFLVRRGESTLFLALRPQG"
     gene            4279..6594
                     /locus_tag="VF089_04075"
     CDS             4279..6594
                     /locus_tag="VF089_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX6173167.1"
                     /translation="MKKSNLRFFSISLIVLLLITSGGIVLGSWYLRILEDTVTAKFDG
                     QKWRFPSKIYSDSYLLYVGMSLRAEDFVEKLRRLGYHETKAAPDGKGEYRFQRSSGLL
                     DIYLHDFSYPTETFKGIPIRISLQGNTVGKLQNGNSGEELFSLELEPELVTGLYETVW
                     QERRLVKLPEVPPLLVRAILAIEDERFYRHVGVDPVALMRAVWVNFRSGAVVQGGSTL
                     TQQLVKNFFLGSERTISRKLKEAFMALIVERNYSKNEILENYLNEIYLGQKGSQGIYG
                     IWEASQFYFSKPLTELSTGEIALLAGLIRGPNNYSPYRSVEAATRRRNVVLAKLLEDN
                     IISRRQYEASVNETLPHRELVKVTNDAPYYVDFLRRELAENYSNDVLTTEGLRIFTSL
                     DLHLQKLAERSLSEGLKRLETKYPALRRRGEDDSLEGAVVVIRPQTGEIKAMAGGRNY
                     QKSQFNRVFQAKRQPGSIFKPFVYLAALMYGADNGRKFTAMTKVDDSPFIWEYDRQEW
                     KPGNYNDEFFGTVTLRRALEKSLNSATARVAREVGIRRVRDLAQRLGIQSSLPAVPSL
                     ALGAAEVTPLEVAVAFSTLANNGVRTRPLTVKQVLDPNARVLEKRDVRVEKVVSAQMA
                     FLINHLLKGVLDRGTADSARRAGFTRPAAGKTGTTNDYKDAWFVGYTPDLLTVVWVGF
                     DNQSKLGLSGSQAALPIWTDFMIRATAGTPVTDFVAPPGIKFLDVDPISGQVATASCT
                     SVIREAFIEGEEPALTCALHPGEQVQAPRLE"
     gene            6810..7382
                     /locus_tag="VF089_04080"
     CDS             6810..7382
                     /locus_tag="VF089_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6173168.1"
                     /translation="MPPDPKIREQDLKTTERVQPSISTAKEIPAPITPPETLTPAKPP
                     VADDSSLLAKIIPGTPPQRAASLRLSEEARKLIDGGEYARALSRLERAIALDSTNAYG
                     HFLLAKTQFGLKRYKDSLNFLDVAESRLGAEPFWLSEVYALRGENFRALGMVERAEES
                     YAKALEINPDNRTVAEALFPNRPVTQPAQR"
     gene            7395..>7619
                     /locus_tag="VF089_04085"
     CDS             7395..>7619
                     /locus_tag="VF089_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX6173169.1"
                     /translation="MLVLGIETSCDDTAAAVLLHGRTILANVISSQDQIHGAYGGVVP
                     ELASRQHIRNILPIIDGALGKAGVTLKDLGG"
BASE COUNT         1786 a         1994 c         2119 g         1720 t
ORIGIN      
        1 gcttagtttt gtgtccaacg attctcccca aacaattctg tgctaagata gtgttcccct
       61 ttatctgcaa acacagtaac aacaatctgc ccttttccca aggtcttggc gacgcggagc
      121 gcggcgcaac aggccgcgcc ggaagaaatg cccaccagaa gaccttcttc agaggctaga
      181 cggcgtgtgt aattaatcgc gtccgtatca ctgaccggaa taacttcgtc aataacttga
      241 cgattcagaa cagccggtat gaaacctgcg ccgagtccct gaatcttgtg ataacccggc
      301 tccccgccgg aaattaccgg cgatgaagcc ggctcaaccg cacagattct cacagcttta
      361 aaacggtctt tcagtacttc gcccactccg gtgatcgttc ctccggtacc gactccggca
      421 acaaaagcat caatccggtt aaactgtttt aacagttcca ttgccgttgt acggcgatga
      481 gcttcgggat tggccgggtt gttgaactgc tgaggcatga agtaatcact gttttcttgc
      541 acgagttctt cggctttatg aatagccccg cccattccct tagtatccgg tgtaagaatc
      601 aaacgcgctc catatgcggc aagcagactg cgacgttctt cgctcatggt atccggcatc
      661 gtcaaaacca aacggtagcc tttcacggct gcaacgagcg ctaaaccgat gccggtattg
      721 ccactagtcg gctcgacaat cgttgagctc ggtttcagct tgccctcacg ttcggcggct
      781 tcaatcatgc tgcaacagat gcgatctttc acagagccgc cgggattaaa cgcctccaac
      841 tttgcccaaa cctcggcgtc atctttaccg ggcaaccttt tcagtttcag gacgggtgtg
      901 gagccgatca ggtccaggat ggacccagcg ccgtttaacg ttatcaatgc tgtgctcgac
      961 tttcctaaga gggaacggag ctcgaataga ttcgcgggta tgctatatac ttgccaaatt
     1021 aaagtcaatt aattgcacgc gagtacccaa tgagcaatcg acgagcgctg gttgtctttc
     1081 ccggggcgtt aggagacttc gtttgctttt tgccggcttt cgaagttctt gctgaacgag
     1141 cgtcaatcga tttgctggct cggacggaat tctctgagct tgttccggcg cgcgtaaatg
     1201 tcgcatcatt agaacgcttt gagattcgcc acctatttgt ctccggcggc ggcagtgatg
     1261 agaaggtgcg aagctttttc gaaacctaca gctgtactta ttcgtggatg ggcagtggag
     1321 atttgacctt taagcaggag cttcaagcgg ctacccaggg ccggatccga atcttccagt
     1381 tttatccgag aaactcgctg atgcaccagg cggattatta tctctcgtgc ctccgcgcga
     1441 attcggctcc gggtatgcct catatttctc tgatgccgga tgcgaagcaa tgggcggaag
     1501 cttactgggc caaacattcg ctcgaatcca gccccgttct cgccatcgcc ccaggaagtg
     1561 gcgcgcgtga gaaaaactgg ccggtgattt cattcgaggc catcgcacgc tggtggcgag
     1621 agcgggttgg cggcagcatc attacgattc ttggaccggt ggaagacgag cgcggaggct
     1681 atgagtctct atgtaattac gggctagcga tacgcggcat taacttggga catttgacgg
     1741 caacgatcgc gcgctgcagt ctgtatgttg gcaatgacag tggcatgtct cacatcgctg
     1801 cagctctcgg agttcccaca gcgaccgtct ttggaccctc aagcattaat cagtgggcgc
     1861 cacgaggaaa cactgtcttg gtactcagac atgaggtgcc gtgctctcca tgcccggttt
     1921 ccgcgatgaa gaattgcgat catcgtgatt gcttagcggc tctgacaccc gaagaagtca
     1981 ttcgaaaact tgagtcttgg tcaccgctcg ttaccttgac aagctggggg gcagggatta
     2041 gagttcttcc taagatttcg cctgaattaa ccggaggata gagcagttga atgaccgctt
     2101 caggcggttt aagcagtgaa tgacgaaaca catttagggt catacgagag tgcctgggaa
     2161 catgttgaat gcgtacaaag ttcccaggct tttttctctt ttaaagaggg tgccatggcc
     2221 ggagaggaag aaaagaaaga aggcaaaggg ttcactgtac aagatcgacg gcggttctct
     2281 gaaacagggg acagccgtga tgacgctccg tcgtcggcag atgctggcga aagcgtccaa
     2341 agaaaaccca ccgaagatgc aacgagccgg tcgccaggca aagccgagga gcaagaatca
     2401 gcattgccgg agatcaattt ttcaaccttt atcatcagcc ttagcacgca agcgctcatg
     2461 catctcggcg agatcgccaa tccgctgacc gggaaggtag aaccggaccg gccagtggcg
     2521 aagcagatga tcgatatcat cggcatgtta cgcgaaaaaa cccgcggtaa tttaaattcc
     2581 ggggaggatc ggctgataga agacattctc ttcgacctcc gaatgaaata cgtcgaggcg
     2641 gtgaagaaga agtaggagca gttgatggac atcaagaggg tctggagctc gagtttcaca
     2701 ttcaagggcg ttgcggttgc tgcaatcgct tcgctactgc tcggcctggc catcagcggc
     2761 agcttcgatt ggctcgcgcc aagccgagcg gtcaacatgg gagatggtgc aagctccgac
     2821 gtccgaatcg ccaatcagtt gcctgatttc gtagctcttt cgaaaaaatt gcgcccggtg
     2881 gtggttaaca tatcttcaac tcaggtgagc gaagcgcgca gtcaacaggg cccgcaggaa
     2941 ttcggcagcc cattcggcga ggaagatccg ttcaacgatt tttggcgacg ctttttcggg
     3001 ggtcccattc ctcgcgggcc tcagcgccag cgaagcctag ggtccggatt catcatcgat
     3061 ggcgatggtt caattcttac caataatcac gtggtcgaaa acgcgcagaa gattgtcgtt
     3121 aaactagccg acgaacaaga gtacgaagcg aaagtgatcg gccgtgatcc gaagacggac
     3181 atcgctgtga tcaagattga ggcgaaaaca aagcttaccc ccgccaactt cggtgactcc
     3241 gacggccttg aggttggcga gtgggtgatg gcaatcggca atccttttgg gctggatagc
     3301 accgtaacct cgggtatcgt cagcgccaaa gggcggcata ttgggcaggg gccctacgac
     3361 aacttcattc aaaccgatgc ctctatcaat ccgggaaatt cgggcgggcc gctgatcaat
     3421 ttgcgcggcg aggtgatcgg gattaacacg gctattttca gccgcactgg cggaaatatc
     3481 ggcatcggct tcgcaattcc ggtcaatctg gttaaggaac ttttgccgca gcttcgcggc
     3541 aaaggaaaag tgactcgcgg atacctgggt gttttgattc agaaagtcac gccggagata
     3601 gcggaaagct tggggatgga gaagagccaa ggagcgctgg tggccaacgt ctccaaagat
     3661 ggacccgcgg ataagtccgg agtcaaagta ggcgacgtca tcgttgagtt cgatggaaaa
     3721 gagatcaaag actcaggcga attgccgatt atcgtggcac gcacaccggt cgacaaaaag
     3781 gtcaagatga aggttttgcg cgataagagc gaggtcactc tgaacgtcgc cgtgggggag
     3841 ctcaaggacg aagaaatcgt ggcttccgct cctgaaaaag gggaaatggg cctaaccgtc
     3901 cagaggttga cgccgcagat cgcagaaagt ctcggtttgg agaaaactga tggcatcgtt
     3961 gtctcagcag ttgaaccggg tagcgccgca gatgaagctg gcattcggcg cggcgatgtg
     4021 attgtcgaga tcgatcgcaa gccgattcgc aacctagagg aatataaaaa atccgtcgcc
     4081 agcgttcgca agggcaaagg agtgctgttt ttggtgcgac ggggtgagag cacgctgttt
     4141 cttgctttga ggccgcaggg ctgatcgcct tcaaagattc tgagatgaat taagagggtc
     4201 gggtggaacc atccggccct ctttttgttg atttttgctc agatctgagt gaatattagg
     4261 cgtctcggag tctatcgttt gaagaaatcc aatcttcgct tctttagcat ttccctcatc
     4321 gttcttcttc tcatcacatc gggcggcatc gtgctcggca gctggtatct cagaatcctg
     4381 gaagatacgg ttactgccaa gttcgatggc cagaaatggc gcttcccctc gaaaatttac
     4441 tccgactcat acctgctcta cgtcggtatg agccttcgcg cggaagattt cgtcgaaaaa
     4501 ttgcgccgcc tcggctatca cgaaaccaaa gcagcgccgg acggcaaggg cgagtatcgt
     4561 tttcaacgat cgtccggact cctcgacatc tatctgcacg attttagtta tccgacggaa
     4621 acatttaaag gcattccgat tcggatatcc ctgcagggaa acaccgttgg caaactgcag
     4681 aatggtaaca gcggtgaaga attattttcg ctcgagctcg aacccgagtt ggtgaccggg
     4741 ctctatgaaa cggtttggca agagcgacga ttggttaagt tgccggaagt gccgccgcta
     4801 ctggtccgcg ccattttggc catcgaagat gaacgatttt accggcacgt cggggtggac
     4861 ccggtcgcac tgatgcgtgc agtctgggtt aattttcgta gtggcgcggt ggtccaaggc
     4921 ggcagcacct tgactcagca actggtgaag aatttctttc tcggtagcga acgcacgata
     4981 tcacgcaaac tgaaagaggc ttttatggcg ctcatcgttg agcgcaacta ctccaagaac
     5041 gaaatcctgg agaactatct caatgaaatt tatctcgggc aaaagggttc ccagggaatt
     5101 tacggcatct gggaggcttc ccagttttat ttctccaaac ctttaaccga attgagcact
     5161 ggtgaaatcg cgctgttggc cggactcatc cgcggaccta ataattactc gccctatcgc
     5221 agcgtggaag cggcgaccag gcgacgcaat gtggtactgg cgaagttgct cgaagacaat
     5281 atcatctcac ggcggcaata tgaggcatcg gttaatgaaa ccttgccgca ccgggagttg
     5341 gtcaaagtca ctaatgacgc cccatactat gtcgattttt tacgacgcga attagccgaa
     5401 aattattcca atgatgtgct gaccacagaa ggactgcgga tttttaccag cctcgatctg
     5461 catctgcaaa agctcgccga acgctcgcta tcggagggcc taaaacggtt ggaaacaaaa
     5521 tatcccgcat tgcgccgccg aggcgaagat gacagcctcg aaggagcggt cgtggtgatc
     5581 cgaccgcaaa ccggggagat caaagcaatg gccggtggtc gcaactacca gaagtctcag
     5641 ttcaatcgcg ttttccaggc caagcgccag ccgggctcga tcttcaagcc attcgtctac
     5701 ctggcggcgt tgatgtatgg agctgacaac ggaaggaagt ttaccgcgat gaccaaggtg
     5761 gacgactctc cgtttatttg ggagtatgat cggcaggagt ggaagcctgg taattacaac
     5821 gacgagttct tcggtactgt gacgcttcgg cgggcgctcg agaagtcact caactcggca
     5881 accgcccgag tcgcccgcga ggtgggtatc cggcgcgtcc gcgacttggc gcagcgtctg
     5941 ggtattcaga gctcgttacc ggctgtgccg tccctagcgt tgggcgcagc ggaagtgacg
     6001 ccgcttgaag tggcggtggc tttctcgacg ttagcgaaca atggcgttcg cacgcggcca
     6061 ttaaccgtta aacaggtgct tgaccccaac gcacgcgtgc tggagaaacg cgatgtccgc
     6121 gtagaaaaag tcgtatcggc gcagatggcg tttttaataa atcatcttct caaaggcgtg
     6181 ctcgatcgag gtaccgcgga tagcgcgcgg cgcgccggat tcacgcggcc tgctgccggc
     6241 aagaccggaa cgaccaacga ttacaaagat gcatggttcg tcggatatac tcctgatcta
     6301 ttaaccgtgg tctgggtggg attcgacaac cagagtaaac tcggtctctc gggctctcag
     6361 gctgcgctgc caatctggac cgattttatg attcgcgcca cagccggaac tcctgtgacg
     6421 gattttgtcg cgcctccagg gatcaagttt ctcgatgtcg atccgatcag cggccaggtc
     6481 gcgacggcaa gctgtacgtc tgtaatccgt gaagctttta tcgagggaga agaaccggcg
     6541 ctcacatgcg ctttgcatcc gggagagcag gtgcaagctc ccaggttaga atgatgctcg
     6601 cttcacgtaa ttcgaatcta ccagaaaaaa gctgcccgaa gctggcaatg gccattgccg
     6661 ttgccttgtg catcttggct gtccagagct gcgcgccgca acggccttac cgcagacctc
     6721 ttccgcctgc agcggctaag cctcagcctg tcccggcgcc aatgcccagt cctccgactc
     6781 cttggccgga ttctgagccc agtcggtcca tgcctcccga tccgaaaatt cgcgagcaag
     6841 acctgaaaac aactgaaagg gtgcagcctt cgatatcgac cgcaaaggaa ataccggcgc
     6901 cgattacacc tcctgaaact cttacgccgg caaaaccgcc ggtggcagac gacagctcac
     6961 tgttagccaa gatcatcccc ggaacaccac cccagcgagc cgcatctctg cgcttgtccg
     7021 aagaagcgcg gaagctgata gacggcgggg agtatgccag ggcactgagc cggttggagc
     7081 gcgccatcgc gcttgattcg acgaacgcct atggccactt cttgttggcc aaaacccagt
     7141 ttggtctgaa gcgctataaa gattcgctta actttcttga cgttgccgag tcccgtctcg
     7201 gcgccgaacc gttttggtta tcggaagttt acgcgctcag aggcgaaaac tttcgtgcct
     7261 tgggcatggt tgagcgcgct gaagaaagtt atgccaaggc tctggaaata aatcccgaca
     7321 atcgcaccgt agcggaagct ttattcccca accgaccggt aacccagccg gcccagcgct
     7381 gatcgttatc ttccatgcta gttctcggta ttgagacttc gtgtgatgac acggcggccg
     7441 cggttctgct ccatggcaga acgattttag ctaacgtcat ctcgtcacag gatcagatac
     7501 acggtgctta tggcggcgtt gtgccggagc ttgcctcgcg ccaacacata cgtaatattt
     7561 tgccgatcat cgacggtgcc ctaggaaaag ccggtgtcac tctcaaagat ctaggcggt
//
LOCUS       DASUTG010000182         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1808910, whole genome shotgun sequence.
ACCESSION   DASUTG010000182 DASUTG010000000
VERSION     DASUTG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808910"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF089_04090"
     CDS             <1..219
                     /locus_tag="VF089_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6173170.1"
                     /translation="LVARDGAEMKQHLQSVLADPQLAGALSGHGRKTILQRHTCAHRV
                     DELSEIYHSIDSSLSSDEALERAPEKHL"
     gene            216..1289
                     /locus_tag="VF089_04095"
     CDS             216..1289
                     /locus_tag="VF089_04095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6173171.1"
                     /translation="MKISFFGSSLLSAYWNGAATYYRGILSALFERGHQIVFYEPDAW
                     DRQRHRDLTEAPYAQSVVYPVDDGSTVLEIVKKAARESDFLVKASGVGVFDALLEEAV
                     LDLRRPDSIVAFWDVDAAATLERIAQNTADPFRSLIPLYDLVLTYGGGEAVVQSYLGL
                     GARRCLPIYNALDPSTHHPAPADPSYDGALSFLGNRLPDREARVFEFFFGPARANPRM
                     RFILGGSGWEDAVPAYANVAYLGHVYTAAHNAFNSTPMAVLNVCRESMARCGYSPPTR
                     LFEVAGAAGCLITDAWDGIEDFLEPGRECLVANDGDEVGEQLRWLTRAQAKEIGQAAL
                     RRILGEHTYAHRAAQLELLLHDL"
     gene            1286..2362
                     /locus_tag="VF089_04100"
     CDS             1286..2362
                     /locus_tag="VF089_04100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6173172.1"
                     /translation="MITQPLDIVILGLSITSSWGNGHATTYRALVRGLEQRGHQVLFL
                     ERDMPWYAAARDLAEARRGRTVLYRSLPQLKKRFHETVRDADLVMVGSYVPDGIALGQ
                     WVVETARGVTAFYDIDTPVTLAAVERGESFYLSADLIRRYQLYLSFTGGPILQRLERQ
                     YGAPMARALYCAVDADLYRPLALPTRWDLGYMGTYSADRQQVLQRLLLDPARRRPDAR
                     MVVAGPQYPSSIEWPANVERIEHLAPCAHPTFYNQQTYTLNLTRANMVHAGYSPSIRL
                     FEAAACGCPIISDWWEGLDNFFKVGDEILVADSPEKTLEYLFDLSAPERELIGERARE
                     RVLSEHTAPRRAAELEGYTRQLLD"
     gene            complement(2533..3348)
                     /locus_tag="VF089_04105"
     CDS             complement(2533..3348)
                     /locus_tag="VF089_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6173173.1"
                     /translation="MQRKLLILSGIIFSGTIALNGNLVWSQTKGGSGTPGAAGQESSD
                     KPTSKGSGASSAPSSSQDRSGSATGSGSASGGSVGSSAAGSSGSGSAMRSGGSASGAA
                     SARGMSGQSSENIKQVQEALKDKGHDPGPVDGVMGPKTQQALRAFQKESGMQATGRLD
                     DKTASALGVDAAGSGKGSPSSGSSSSMDKSSSGSGASSSGRSSATEKSSSGGSSTSPS
                     SSGSSGSSLGTGGSSGAGSGSSKSDSSGGSSKGGSSSGGSSTGSPSGSGSSGS"
BASE COUNT          688 a         1103 c         1066 g          857 t
ORIGIN      
        1 ctggtcgcac gtgacggcgc ggagatgaag cagcacttgc aaagcgtgct tgctgatcct
       61 cagctcgccg gcgcattgtc cggccatggg cgtaagacca ttctgcagcg tcatacctgc
      121 gcccacaggg tcgacgaact cagcgagatc tatcacagca tcgactcgtc cctgtcatcc
      181 gatgaggccc tggaacgagc gcccgagaag catctatgaa aatctcgttt tttggctcga
      241 gtctgctatc ggcgtactgg aacggcgcgg cgacctatta ccgaggaatt ttaagcgcgc
      301 tcttcgagcg ggggcatcag atcgttttct atgagcctga tgcttgggat cgccagcgtc
      361 accgggatct caccgaagcc ccctatgcgc agtccgtggt gtatccggtc gacgatggct
      421 cgaccgttct cgagatcgtc aagaaagccg cgcgcgaatc ggatttcttg gtgaaggcca
      481 gcggcgtggg tgttttcgat gcgctccttg aagaggctgt tctggacttg cgccgtcctg
      541 acagcatcgt tgccttctgg gatgtcgatg ccgcggcgac actggagcga atcgctcaga
      601 ataccgccga tccattccgc tcgctgattc cactctacga tctggttctt acctatggcg
      661 gcggcgaggc agtcgtgcag agctatctcg gtctcggcgc ccggcgatgc ttgccgattt
      721 acaatgcctt agatccgagc acgcaccatc cggcgcccgc cgatccaagc tatgatggcg
      781 cgctgagctt tctcggtaac cgcctgcccg atcgtgaggc gcgggtcttt gaattctttt
      841 ttggaccggc gcgcgccaat ccgcgcatgc gctttattct tggcggcagc ggttgggagg
      901 atgcggtgcc ggcgtacgcg aacgtcgcct atcttggcca cgtctatacc gcggcacaca
      961 acgcattcaa ttcaactccc atggccgttc tgaatgtctg tcgggaaagc atggcgcgct
     1021 gtggctactc tccaccgact cgcttgttcg aggtcgccgg cgccgcgggc tgtctgatta
     1081 cggatgcctg ggacggtatc gaagattttc ttgagcccgg ccgtgagtgt ttagttgcca
     1141 atgatggcga cgaggtcggc gagcagctgc gatggttaac gagagcacaa gctaaagaaa
     1201 ttggtcaagc tgctctgcgc cggattctag gcgagcatac gtacgcgcac cgtgcggctc
     1261 agctggaatt gttgctgcac gacctatgat tactcagccg cttgatattg tgatcctcgg
     1321 tctttccatt acgtcgtcgt ggggcaatgg acatgcgacc acttaccggg ctctggtgcg
     1381 cggactcgaa cagcgcggtc atcaggtttt atttctcgaa cgcgatatgc cttggtatgc
     1441 cgccgctcgc gatttagccg aggcgcgccg gggacgaacg gttctttacc gtagcttacc
     1501 tcaactcaag aaacgctttc acgagacagt gcgcgatgcc gatctggtta tggtgggctc
     1561 gtacgtgccg gacggaattg cgcttggtca atgggtcgtg gaaactgcac gcggtgttac
     1621 ggctttttac gatatcgaca cgccggtgac tttagctgcc gtggagcgtg gcgaatcttt
     1681 ttacctttcc gccgacctga tccgccgata tcagctgtat ctctcgttta ccggcgggcc
     1741 gattttacaa cgactcgaac ggcagtatgg cgcgcccatg gcccgagcgc tgtattgcgc
     1801 ggtcgatgcc gatttatacc ggcctctggc gctgcccacc cgatgggact tgggctacat
     1861 ggggacttac agcgccgatc gccaacaggt tctgcaacga ctgctgctcg atcccgcccg
     1921 ccgtcgccct gatgcgcgca tggtggttgc cggccctcag tatcccagct ctattgagtg
     1981 gcctgccaat gtcgagcgca tcgagcatct ggcgccatgc gcacatccga ccttctataa
     2041 tcaacaaact tataccctca atctaactcg ggccaacatg gttcacgccg gttattcacc
     2101 aagtattcgt ctatttgaag cggctgcttg tggatgtccg atcatcagcg actggtggga
     2161 ggggctggat aattttttca aggtggggga cgagattctg gtagcggact caccggaaaa
     2221 aaccctcgag tacctcttcg atctgagcgc gcccgagcgt gaactgatcg gcgagcgagc
     2281 ccgcgagcgc gtgctgtctg aacacacggc gccgcgtcgt gccgcggagc tcgaaggcta
     2341 cacccgccag ctgctcgatt gattcgtaag ctcccgcaaa acagtttaaa tgtttgaaac
     2401 accgtagaac gccgagcagg gaggaccaaa acctccctgc tgtcgcgccg attggcgttc
     2461 gctgagactt gagccaacta gagataaacg caacgccacg cgcttatcac tttctgactt
     2521 cgtactcgac gattagctgc cggaggagcc actacccgaa gggctgccgg tggatgagcc
     2581 gccgctggaa gagccacctt tggaggaccc gccgctggaa tcagacttgg aagagccgct
     2641 cccagctccc gatgagccgc cggtgcccaa cgaactgcct gacgaaccgc tgcttgacgg
     2701 gctggtagaa gagccgccag aggacgactt ctcagtagcc gagctacgcc ccgatgagga
     2761 agctccactg ccggacgaac tcttgtccat actggaggaa cttccgcttg agggagaacc
     2821 ttttccgctc cccgcagcat ccactccaag cgcgctggcg gtcttgtcat cgagccgtcc
     2881 cgttgcttgc ataccgcttt ctttctggaa ggcacgcagg gcttgctgcg tctttggtcc
     2941 catcacgccg tctacgggac ctgggtcgtg tcccttatct tttagcgctt cctgaacctg
     3001 cttgatgttt tcactggatt gtccgctcat tccgcgcgcg ctcgctgcgc cgctcgcgct
     3061 gccgccgctg cgcattgcgc tgcccgaacc gctcgagccg gcagcagaac tgcccaccga
     3121 cccaccagaa gcgctacctg atccggtggc agacccgctt ctatcctgac tgctggatgg
     3181 tgccgaggac gcgccgcttc ccttgctcgt cggtttatcc gagctttcct gtcccgcggc
     3241 gcccggcgtc ccgcttcccc ctttggtttg tgaccacacc aggttgccgt taagtgcaat
     3301 tgtaccgctg aaaataattc ccgatagaat taacagtttt cgctgcatca ctttttctcc
     3361 tttcgttatg actgtggcgt tgcgtccgtg aatagctgtg caacggatat gccacctatc
     3421 gagtaatcat aacgtcgcat tctcgacaac ccactcgacc ggatcgaaga ctgttgcgga
     3481 ttctgtccca aacacgcgtt aaggagactt gggtgccatc cgcaattggg acgacgttgc
     3541 agtatgaagc aggcgccaaa cagattctga cggatggtgt ctgctgattg tagatcgagt
     3601 cggatcctcg tggccttcaa agtcgttttt acattcgcaa gcggttggtg attgctgccg
     3661 tcggaaccag gtttgtttca tctcgggcgg gtgcgcgctt ccgagtggat cgag
//
LOCUS       DASUTG010000183         7766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_29974, whole genome shotgun sequence.
ACCESSION   DASUTG010000183 DASUTG010000000
VERSION     DASUTG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29974"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2800)
                     /gene="carB"
                     /locus_tag="VF089_04110"
     CDS             complement(<1..2800)
                     /gene="carB"
                     /locus_tag="VF089_04110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX6173174.1"
                     /translation="MPKRTDIKSILLISSGPIIIGQACEFDYSGTQALKALKEEGYRV
                     ILVNSNPATIMTDPEFAHRTYIEPITTDIVEKVIAKERPDALLPTLGGQTALNLAIDL
                     AEKGVLARYGVEMIGAKLESIKKAEDRDLFKEAMKRAGLDLPRSGYARSMRDAIRIQK
                     RLDFPVIIRASRTLGGSGGSFAFGPEEFERVVQAGLSISPVHEVLIEESIIGWKEFEL
                     EVMRDHKDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRDASLRVIREIG
                     VETGGSNIQFAVNPKDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IRNDITRETPACFEPTIDYVVVKIPRFTFEKFPQAKDLLTPQMKSVGEAMAIGRTFKE
                     SLQKAMRSLEIGSFGFEPRVDLSPDRRDQALDTVREKLKNPNSQRLWYLADAIRLGMP
                     AEQIYELSHFDPWFVEKVREIVATEGEIADYRGKLNEVDSEAIRDWKQMGFADPRIAN
                     LTGTTEDEVRRQRKSQGIEAVFCSVDTCGAEFEAFTPYLYSTYEGEDESKPTQRKKIM
                     ILGGGPNRIGQGIEFDYCCVHAAFALKEDGYETIMVNCNPETVSTDYDTADKLYFEPL
                     TLEEVLNIVDRENPEGVIVQFGGQTPLKLALPLERAGVKIIGTSPDSIDLAEDRERFS
                     ALLNRLGIRQPDNGIARSYEEAFHIAERLGYPVLVRPSYVLGGRAMEIVYDADALRRY
                     MTYAVSASPEHPVLVDKFLDDAIEIDVDALSDGDQVIIGGIMEHIERAGVHSGDSACS
                     LPPKSISVEVQDVIRQHSIALAEALNVRGLMNVQFAVQGKNVFVLEVNPRASRTVPFV
                     SKATGVPLAKIAARVMVGRKLRELGINMEVIPKHISVKEAVFPFNKFPGVDTLLGPEM
                     KSTGEVMGIDANFGVAFAK"
     gene            complement(2811..3986)
                     /gene="carA"
                     /locus_tag="VF089_04115"
     CDS             complement(2811..3986)
                     /gene="carA"
                     /locus_tag="VF089_04115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX6173175.1"
                     /translation="MERKKAILALADGRCFEGWSFGAEGEAAGEVVFNTSMTGYQEIL
                     TDPSYEGQLVAMTYPQIGNVGINPEDVESHRPFVKGFIVKEYTAVPSNWRATQPLDVY
                     MQQHGIVGIEGIDTRSLVRHLRDCGAQVGVITTVSDNSDEAIAKAKAAPGLEGRDLVR
                     NVSCASAYEWDQGLWRLGEGYRVQGNEARRKKNSTRGERITVPRHSVVAYDFGIKYNI
                     LRNLAESGCRVKVVPAHTSAEDVLAFNPDGIFLSNGPGDPDAVSYAKENVRKLIGRKP
                     IFGICLGHQILGLALGGRTYKLKFGHHGGNQPVMDLTTRKVEITSQNHGFAVDADSLK
                     GSVEVTHLNLNDNTVEGLVHRDLPIFSVQYHPESSPGPHDANYLFRRFIELMDQHQG"
     gene            complement(4084..5994)
                     /locus_tag="VF089_04120"
     CDS             complement(4084..5994)
                     /locus_tag="VF089_04120"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX6173176.1"
                     /translation="MTTANLVSIADLSNEEIEEIFDLADASDKLRSKQVGRGKIMATL
                     FYEPSTRTRLSFESSMQRLGGTVISCSDMQSSSAAKGESVADTAKVVSSYADVLVVRH
                     YWDGAVQAVAEHADVQVINAGDGGHEHPTQTLCDLYTLRKEKGELKGLNVVICGDLKN
                     GRTIHSLVYALARFGANVVTLAANGMELPHYVLERLEREFQYSLAPVASDDLSAVVTE
                     TDALYLTPKEPHQLALFTEVDSTIQTRITSLVSGLRYDAFYMTRKQKERMTNVAHAGN
                     YPVIGSKFLKEQRFKDTVVMHPLPRIDELSPELDKDRRGIYFKQAAYGVPIRMALLHF
                     LFEKQRRMQIDTPVENSVLFESPHPVGPHCPNVRCITVNEPGSTRRRFELLSEGPAGA
                     LILRCLYCEHRFKVQLVGETTTKRYCAYDIALADNIARWLKSGELAIFDSIKQAEELG
                     YGPYKSGPRRAIMDQAQVESAVQQISEQILRDGGDPDRLLIVGVKTNGSMIAQRIAAN
                     LERAHGRKFEVAELETYGAGEDLRRLSPADPELGPMQLRDRSIILVDDVIHTGRTVKT
                     ALSMIFRSGRPQSVRLAVLIDRGHRQIPVKPNYVGKHIPTADHERVRVHLRELDTDGK
                     DKVVVYSITHPS"
     gene            complement(6005..6553)
                     /gene="pyrR"
                     /locus_tag="VF089_04125"
     CDS             complement(6005..6553)
                     /gene="pyrR"
                     /locus_tag="VF089_04125"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6173177.1"
                     /translation="MTSEKQIVLTKQELAKAITRMAHEVLEKAGSFEELTLIGIRSRG
                     VHIARRLARKIEELTEHSPPLGVIDVTLFRDDKIQTQASTSASAGEISVPLENKIAVL
                     VDDVIFRGRTVRAALEAVERLGHARRIMLAVLIDRGARELPIRADIVGKNIEASDSQR
                     VNVLLEESDGIDQVIVMPWKPV"
     gene            complement(6754..7248)
                     /locus_tag="VF089_04130"
     CDS             complement(6754..7248)
                     /locus_tag="VF089_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017363151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX6173178.1"
                     /translation="MATRKQRNFALEEGRIGLISDTHGLVRPEALAALKGVELIVHAG
                     DIGKPEVLKRLQAIAPLAAIKGNNDTGPWARHIPEILDVHVKDRTLRVIHYLRDSQSN
                     LRAAGIDVVISGHSHKPSVESRDNVLFVNPGSAGPRRFKLPVTVGLLLLENADVRAEI
                     IHLL"
     gene            7309..>7766
                     /locus_tag="VF089_04135"
     CDS             7309..>7766
                     /locus_tag="VF089_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173179.1"
                     /translation="MLPHLKGFNLQQWIDENRNNWGQRRVIWNDSDFIAFVTRGPNRR
                     KDFHINPGDEIFYQLEGELNLHYLSDGKPELAVLKTGDLFLMPARVPHSPRRREGSWT
                     FVIERKRTHEERDLFIWVCEKCQKSLYETDVRFDDPSDAVAKASEAMRRD"
BASE COUNT         1729 a         2211 c         2020 g         1806 t
ORIGIN      
        1 cttttgcgaa cgcaactccg aaattggcgt cgatccccat cacctctccg gtagatttca
       61 tctccggacc cagcaaagta tcgactccgg gaaatttgtt gaacggaaag accgcctcct
      121 taaccgaaat gtgcttgggt attacctcca tattgatccc aagctcgcgc agcttgcggc
      181 cgaccatcac ccgcgccgcg atttttgcca gaggcacacc ggtggctttg ctgacaaagg
      241 gcaccgtacg ggaagcgcga ggattgacct cgaggacgaa gacatttttg ccttggaccg
      301 cgaactgaac attcatcagc ccgcggacat tgagagcttc ggccaaggct atgctatgtt
      361 gccggatgac gtcttgaacc tccacgctga ttgatttggg cggcagacta catgcgctgt
      421 cgccggagtg cacgccggcc cgttcaatat gttccatgat cccgccgatg atgacctgat
      481 ccccgtccga taaagcatcg acgtcgattt cgatcgcatc atcgagaaat ttatccacta
      541 gcaccgggtg ctccggtgat gccgataccg cataggtcat gtagcgccgc aacgcgtccg
      601 catcataaac gatttccatt gcccgcccgc cgagcacgta agagggccgc accaacaccg
      661 gatatcccaa gcgctcggcg atgtgaaagg cctcttcata ggaacgcgcg atgccgttat
      721 cgggttgtct gatcccaagt cggttgagca gcgcggagaa gcgctcgcgg tcttcggcga
      781 gatcgatgct gtccggtgag gtaccgatta tttttacgcc ggcgcgttcg agcggcaacg
      841 ccaatttaag cggcgtttgc ccgccgaact gaacgatgac accctcggga ttttctcggt
      901 cgacaatatt cagcacctct tctaaagtta gcggttcgaa gtagagcttg tccgcggtgt
      961 cgtagtccgt gctcacagtc tcagggttgc aattgaccat aatggtctcg taaccgtctt
     1021 ctttgagcgc aaaggcggca tgcacgcagc agtaatcaaa ttcgatgccc tggccgatcc
     1081 ggttggggcc gccgccgaga atcatgattt ttttacgttg cgttggcttt gactcatctt
     1141 cgccttcgta agtcgaatag agatagggag taaaggcttc aaactcggcg ccgcaagtat
     1201 ccaccgaaca aaataccgcc tcaatcccct gagactttcg ttgacggcgc acctcgtctt
     1261 cagtcgttcc cgtcagattg gcaatccgag gatcggcaaa gcccatctgt ttccaatccc
     1321 gaatagcctc gctgtcgact tcgttcaact tgccgcgata atctgcaatc tcgccctcag
     1381 tggcgacgat ctcgcgcacc ttttccacga accatgggtc aaaatgggac agctcataga
     1441 tctgttcggc cggcatgccg agccggatcg catcggccaa ataccatagc cgttgcgaat
     1501 tcgggttctt cagtttctca cgaaccgtat ccaaggcctg atcacgtcgg tcaggcgata
     1561 ggtcgacccg gggctcaaag ccaaacgaac cgatctcgag agaacgcatg gctttctgca
     1621 gtgactcttt gaacgtgcgc ccaatcgcca tggcttcgcc gaccgacttc atctgcggtg
     1681 ttagcaaatc tttggcttgg ggaaactttt cgaacgtaaa gcgtggaatt tttaccacga
     1741 catagtcgat agtcggttcg aaacaggcag gcgtttcccg cgtgatatcg ttacggatct
     1801 catctagcgt gtagccgatt gccagcttgg cggcgatttt cgcgatgggg aaaccggttg
     1861 ctttgctcgc caaggccgag ctgcgcgata cccgcgggtt catctcaata accaccatgc
     1921 ggccatcttt cgggttgacc gcgaactgaa tattggaacc cccggtttcg acgccgattt
     1981 ctcgaatcac gcgcaatgag gcatcgcgca taatctgata ttctttgtcg gtcaaggttt
     2041 gcgccggcgc aaccgtgatc gaatccccgg tatgcacgcc catgggatcg aaattttcga
     2101 tcgagcagat gatcaccaca ttgtccttgt gatcgcgcat gacttcgagc tcaaattctt
     2161 tccagccgat gatcgactct tcgatcaaca cttcgtgcac cggagagata ctcaagcccg
     2221 cttgcacaac ccgttcgaac tcctccgggc cgaaggcaaa actgccgccg ctgccgccga
     2281 gcgtgcgcga ggcgcggatg atgaccggaa aatcgagccg cttttgaatg cgtatggcat
     2341 cgcgcatgct ccgcgcatag ccgctgcgcg gcagatccaa accggctctt ttcatcgctt
     2401 ccttgaacaa atcccggtct tcggccttct taattgattc cagtttggcg ccgatcatct
     2461 cgaccccgta acgcgccagg actcccttct ccgccagatc gatcgccaaa ttcagcgcgg
     2521 tttgtccgcc caatgtgggc agtagtgcgt ccggccgttc cttggcgatc accttctcga
     2581 cgatatccgt ggtgattggc tcgatgtagg ttcgatgcgc aaactccggg tcggtcatga
     2641 tggtcgccgg attggagtta accagaatga cccggtagcc ttcctcttta agcgccttga
     2701 gcgcctgtgt gccggaataa tcgaactcgc aagcctggcc gattatgatc ggacccgagc
     2761 ttatcagcag gatcgacttg atatcagtgc gtttcggcat cgaaaagttg ctagccctgg
     2821 tgttggtcca tcaattcgat gaagcgtcga aaaagatagt tggcatcgtg cggcccgggt
     2881 gaggactccg gatgatactg caccgagaag atcggtaaat cccgatgcac caaaccctcc
     2941 accgtattgt cgttgagatt gagatgggta acttcgaccg atccttttaa ggaatcggca
     3001 tccacggcaa agccgtggtt ctgcgatgtg atctctactt tgcgtgtggt taagtccatc
     3061 accggctggt tgccgccatg atgaccaaat ttaagtttat aggtcctgcc acccagagcc
     3121 aagcccaaaa tctggtggcc caggcagatg ccaaagatgg gtttcctgcc gatcaacttg
     3181 cgcacgttct ctttggcata agacaccgcg tccgggtcac ccggtccatt ggacagaaat
     3241 atgccgtcgg gattaaaggc caaaacatct tctgcggaag tatgggcagg aacgactttc
     3301 acccggcaac cgctctcggc gagatttcgc agtatgttgt acttgatgcc aaagtcgtaa
     3361 gccaccacag aatgccgagg aacagtaatc cgttcgccac gcgtggagtt ttttttgcgc
     3421 cgggcctcgt taccctgcac ccgatatcct tcacccaatc gccacaaacc ttgatcccat
     3481 tcataggcgc tcgcacagct cacgttgcgc actagatccc ggccctcaag tccgggagcg
     3541 gccttggctt ttgcaatcgc ctcgtcggaa ttgtcagaaa ccgttgtgat gacgcccacc
     3601 tgcgcgccac aatcgcggag atgacggacc agagaacgtg tgtcgatccc ttcgattcca
     3661 acgatgccgt gctgctgcat atagacatcc agcggctgtg tcgcgcgcca attactcggc
     3721 accgcggtat actctttgac gatgaaccct ttgacaaagg gccggtggga ttctacgtcc
     3781 tcgggattaa tgccgacgtt gccgatctgc ggataagtca tggcgacgag ctgaccttcg
     3841 taagacggat ccgtgagaat ctcttgatag ccggtcatgc tggtgttaaa cacgacctcc
     3901 cctgcagctt cgccctcggc gccaaaagac cagccctcga aacatcgccc gtcagccaag
     3961 gccaatattg ctttctttct ttccatcgat tccgttctga ctcctgctca aaaactcagg
     4021 taaaagatga tcaaatacgc aaagttgaag agtaagcata atgtaatttc acgcaatttt
     4081 gaatcagctt gggtgggtaa ttgaataaac aaccacctta tctttcccat ccgtatccag
     4141 ttcacgcaaa tgaacacgca cgcgctcgtg gtctgccgtg ggtatgtgtt taccgacgta
     4201 attaggcttt actggaatct gccgatggcc ccggtcgatt aaaaccgcta gacgcacgga
     4261 ttgtggccgc cccgaacgaa aaatcatgct caaggcggtt ttcaccgttc tccccgtgtg
     4321 aatgacgtca tcgacgagaa tgatcgatcg atcgcgcagc tgcatcggcc ctaactcggg
     4381 atccgcggga gacaggcggc gaaggtcttc gcccgctcca taggtttcga gctcggcgac
     4441 ttcaaatttt cgcccgtgcg ctcgttccag attggctgcg attcgttggg cgatcatcga
     4501 cccgttagtt ttaaccccga caatcagtaa gcgatcggga tcgccgccat cgcgtaaaat
     4561 ttgctcgctg atctgctgca ccgcagattc tacttgagcc tgatccatga tggcgcgtcg
     4621 cggaccgctc ttgtaaggtc cgtagccgag ctcttcagct tgtttgatgg aatcaaagat
     4681 cgccagctcg ccgctcttga gccaacgcgc aatgttatcc gcaagagcga tatcgtaggc
     4741 gcaatacctc ttcgtcgtcg tttcaccgac gagttgaact ttgaagcgat gttcgcagta
     4801 aaggcatcgc aaaatcaatg cgccggccgg gccttcggag agaagctcga aacgacgccg
     4861 ggtcgaaccc ggctcgttga cggtaatgca cctgacgttc gggcaatgcg ggccgaccgg
     4921 gtgcgggctt tcgaagagca ccgagttttc tactggcgtg tcaatctgca tccggcgctg
     4981 cttttcgaac agaaaatgta gcagtgccat gcgaatgggc acgccatacg ctgcttgctt
     5041 gaaataaatg ccgcgccgat ccttgtcaag ctcgggcgaa agctcatcta tccgaggtaa
     5101 gggatgcatc accaccgtgt ctttgaagcg ctgctccttg aggaatttag aaccgatgac
     5161 cggataattt cccgcatgtg caacgtttgt catgcgctct ttttgctttc tcgtcatgta
     5221 aaaagcgtca tagcgcaatc ccgataccag gctggtaatc cgcgtctgaa tcgtgctgtc
     5281 cacttcggta aacaatgcca gctgatgcgg ctccttggga gtgagataaa gcgcatcggt
     5341 ctcggtaacc acggcgctca gatcatcgct tgcaaccggc gccaagctat actgaaactc
     5401 gcgctccaac cgctccagaa cataatgggg tagctccatt ccgttggctg ccagagtcac
     5461 aacattggcg ccgaacctgg ccaatgcgta gaccagggag tgaattgtcc ggccgttttt
     5521 tagatccccg catataacca cgttaagacc tttgagctcg cccttttcct tgcgcagcgt
     5581 gtagagatcg caaagcgtct gggtcggatg ttcatgacca ccgtcgccgg cattgatcac
     5641 ctgaacatcg gcatgttcag ccactgcctg aactgcccca tcccagtagt ggcgaacgac
     5701 cagaacatcg gcataactcg aaactacctt ggcggtatcg gcaacgctct cccccttggc
     5761 ggccgacgaa gactgcatat ccgaacagct gatcacggtg ccgccaagcc gctgcatcga
     5821 cgattcgaaa gataaccgag tgcgggtgct gggttcgtag aacaaggtcg ccatgatctt
     5881 gcccctgccc acctgtttgc tgcgcaactt gtcggacgca tctgccaaat cgaaaatctc
     5941 ttcgatctct tcattgctga gatccgcgat ggaaactaga ttggccgtgg tcacgttcga
     6001 ttcctcaaac cggcttccag ggcatgacaa tcacttgatc aatgccatcg gattcttcga
     6061 gtaagacgtt cactcgttgg gagtccgaag cttcgatatt cttgccgacg atatcggccc
     6121 ggatcggcaa ctcccgggcg ccgcgatcga ttagaaccgc cagcatgatc cgcctggcat
     6181 ggccgaggcg ctccaccgct tccagagccg cgcgaaccgt ccgtccgcga aaaataacat
     6241 catcgactaa gacggcgatt ttattttcca gcgggaccga aatttcaccc gcgcttgccg
     6301 aagtggaagc ttgtgtttgg atcttatcat cacgaaaaag cgttacatcg atgaccccga
     6361 gaggcggtga gtgctcggta agctcttcga tcttacgcgc caaacgtcga gcgatatgga
     6421 caccgcggga acgtatgccg atcagtgtca gctcttcgaa actaccggct ttctcgagca
     6481 cctcatgagc catccgagtg atcgctttgg ccaactcttg cttggtcagg acgatttgtt
     6541 tttcactggt catagaccaa aaaaagaccc cctcaaccgc gttgaggagg cccgaagaat
     6601 cggaatcttg gaagtggaat tacctgcatg gttcccttac cgacctcaca ggaccggttt
     6661 aaaaggattt gctatttatc gcctgacgga aggtcaaagt caatgatgaa atacggctgt
     6721 gagagaaacg gttcattgtt caacaagcca aactcagagc aagtgaataa tctctgcgcg
     6781 cacgtcagcg ttttccaaaa gtaacagccc taccgtgacc ggcagtttga aacgtcgcgg
     6841 gccggcgctt ccgggattaa caaaaagcac attgtctcgg ctttccactg acggtttatg
     6901 cgaatgcccg ctgatcacga cgtcaatgcc tgcggcgcgg agattcgatt gactatcccg
     6961 gagatagtga atcacccgca gggttctgtc tttaacgtgt acatccaaaa tttcggggat
     7021 gtgccgcgcc cagggcccgg tatcgttgtt gcctttgatg gctgccaagg gtgcaatagc
     7081 ctgcaagcgt ttcaacactt cgggcttgcc gatatctccg gcatgcacga tgagttcgac
     7141 gccttttaac gccgcgagag cttccgggcg aaccagtccg tgggtgtcgg aaatcaaacc
     7201 gatgcgccct tcctccagag cgaagttccg ctgttttcgt gttgccatgg tcgctcttga
     7261 ttcctggttg gactcaatcc tatattcgac tgcggccaag gaggttgtat gctcccccat
     7321 cttaaaggct ttaacttaca gcaatggatc gatgaaaatc gcaacaactg gggacaacgc
     7381 cgcgtgatct ggaatgattc ggattttatc gccttcgtga cccgcggtcc caatcggcgc
     7441 aaagactttc atattaaccc cggtgatgaa atcttctacc aactcgaagg cgaactcaat
     7501 cttcactact tatccgacgg caagcccgag ctcgcggtac tcaagaccgg tgatcttttt
     7561 cttatgcccg cgagagtgcc ccactcgccg aggcggcgcg aaggctcctg gacgtttgtg
     7621 atcgagagga aacgcactca cgaggaaagg gatcttttca tctgggtgtg cgaaaaatgc
     7681 cagaagagtc tctacgagac ggatgtccgg ttcgatgatc cgtcggatgc cgtcgcaaaa
     7741 gcgagcgagg cgatgcgcag ggataa
//
LOCUS       DASUTG010000184         4471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2105766, whole genome shotgun sequence.
ACCESSION   DASUTG010000184 DASUTG010000000
VERSION     DASUTG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105766"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VF089_04140"
     CDS             complement(<1..1757)
                     /locus_tag="VF089_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6173180.1"
                     /translation="MNLKFVGRPISRLDGPDKVSGNTVYTADVQLPGMLWGKCLRSPY
                     AHARIVSINTDAAKKVEGVRAVLTGEDFPEKRVGLSLQDTPLLAVGKVRFIGEKVVAV
                     AAEDRDAAEEALSAIEIEYQELPGVFDASQAMTSGAPLVHEQLATYRGFNAPGDEIPN
                     VFAMQKWTAGDIAAGFDEADLIIEHTFRTAVAHQAYIEPHSAIVAVDSSDHVDIWASC
                     KAPFRVKSHLSRQLEIPKERIRVHVVAVGGDFGGKGALMDIPICYELAKATRRPVKMV
                     MTYAEELVAGDPRHSSVIKIRSGVKKDGTLTARQTEVIFNSGAYASFKPGDPPNLGGA
                     TFTNGVYHIPHYSIRSYGVYTNSVPCGYYRAPGQPQAVFAAESHMDLIAEELRMDALE
                     LRLKNLMHDGEKLAGGRTVDKVRVRETLQAAAQKAGWGKKNGKNTGLGIGVSFRHVGG
                     SGTASAELSLSADGKLTILTAVPDIGTGTHTLLRQIAAEVLTVPPELILTEIGDTESF
                     ESDAAPGGSKITNMSGHVVLQAAQQLRERLTPVAASLLGCAVSDVVLQNGRFGSAREK
                     KSAIEFRDVAQEATKASGEL"
     gene            complement(1782..2720)
                     /gene="phnE"
                     /locus_tag="VF089_04145"
     CDS             complement(1782..2720)
                     /gene="phnE"
                     /locus_tag="VF089_04145"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX6173181.1"
                     /translation="MVQRLQATDSPDIFLEAPPAPRHKLRSFIFTVVFLLVLAWAFQG
                     AKIRVGELLEGIPQIAQTLARMLPPDFSKLTDANYYYFPEELSWRELLLPLPLPEASA
                     RIKQRWWNNIFPQTVVGATLETVQMALAGTFTALIVAFPLGFLAARNTTPHPLVYHSV
                     RTVLNLLRTVPDLALGLLFVAAVGLGAFAGTLALAIHTATVLGKLLSESVENIDEGVV
                     EAIRATGAGYTQILSFAVLPQILPDLISFTLYRLETNIRAASVLGLIGAGGIGYLMNT
                     SFRTFQYQEAAAIVLVLIALVMLVDYLSSRLRTLVV"
     gene            3175..4023
                     /locus_tag="VF089_04150"
     CDS             3175..4023
                     /locus_tag="VF089_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6173182.1"
                     /translation="MSDRDALKYGGNTSCVEVVAGKHQIIFDAGTGIIGLGKELVKNN
                     LGPLSLNLFLSHTHHDHLSGFYFFDPLFDKRNRINIFGPGSGKRSLAETLRMAMEPAL
                     FPIRLDALDAKKSIWSLVGGETIALRDNRRAPRITKAVSLRDKDRERPIIITHGSPAH
                     PNGVILYRVHFQGKSLVYATDLEETAGGSPDVIEFARDTDLLIHDAQYLESEYMSTAN
                     PRRGWGHSTVESATEVAKKAGVKQLVLFHHEPTHDDRCIDQILKLARRRFPGTTVAAE
                     GMVMRL"
BASE COUNT         1011 a         1273 c         1213 g          974 t
ORIGIN      
        1 agctcaccac tcgccttggt cgcctcttgg gctacgtcgc gaaattctat cgctgatttc
       61 ttctcccgcg cgctgccgaa gcggccgttc tgcagaacta cgtcactaac ggcgcaaccg
      121 agaagcgatg ctgcaacggg agtcaatcgc tcgcgcagct gttgagccgc ctgcaacacc
      181 acgtggccgc tcatattggt aatcttgctg ccgcccggcg ctgcgtcgct ttcaaagctt
      241 tcggtgtcgc ctatttcagt gagaatcagc tcgggcggaa ccgttaagac ttccgcagcg
      301 atttgccgca gcaaggtatg cgttccagtg ccgatatccg gcacagcggt taagatcgtg
      361 agtttaccgt cagcagatag agaaagctcc gcgcttgctg taccggagcc accaacatgc
      421 cggaacgaga cgccgatgcc gagaccggta tttttaccgt tcttttttcc ccagcctgcc
      481 ttctgcgctg ccgcctgcaa agtctcccgc acgcgtacct tatccaccgt ccggccgccg
      541 gctaactttt caccgtcgtg catgaggttc ttaagccgca attcgagcgc atccatgcgc
      601 aactcttcgg cgatcaaatc catatgagac tcggcggcaa acaccgcctg gggttggccg
      661 ggagcgcgat aatagccgca cgggacagaa ttggtgtaaa cgccatagga ccggatggag
      721 taatgtggga tatggtagac accattggta aaggtcgcgc cgccgagatt cggcggatct
      781 cccggcttaa acgaagcgta cgccccgctg ttgaagatta cttcggtttg ccgcgcggta
      841 agagtaccat ccttcttgac accgcttctg atcttgatca ccgaggaatg gcgtggatcg
      901 ccggcgacta gctcttccgc ataggtcatg accatcttca ccggtctgcg agtggccttg
      961 gcgagctcgt agcagatggg aatatccatc aaggcgcctt tgccgccgaa atcgccgcca
     1021 acggcaacga catggacacg aatccgctct ttggggattt ctaattggcg cgacaggtgc
     1081 gacttcaccc ggaacggcgc cttgcacgat gcccagatgt cgacatgatc cgagctatcc
     1141 acggccacga tcgccgagtg cggttcaata taagcctgat gcgcaactgc ggttctaaaa
     1201 gtatgctcga tgattagatc cgcttcatcg aagccagccg cgatatctcc cgcggtccat
     1261 ttctgcattg caaagacgtt gggaatttca tcgcccggcg cgttgaaacc cctgtatgtg
     1321 gccaattgct catgcaccag gggcgcgccg ctggtcatcg cctggctggc atcgaacaca
     1381 cccggtagct cttggtactc aatttctata gcggagagag cttcttcggc agcgtcgcga
     1441 tcctcggcgg cgaccgcgac caccttctcg ccgataaacc gcaccttgcc gactgctaga
     1501 agcggcgtgt cttgcaggct cagtccgacc cgcttctccg ggaaatcctc gccggtcaga
     1561 acggcgcgca caccttcgac ctttttcgcc gcgtccgtgt tgattgaaac gatgcgcgca
     1621 tgagcatagg ggctgcgcaa acacttgccc cacaacatgc cgggcagctg cacatcggcg
     1681 gtgtagaccg tgttaccgct gaccttatcg ggtccgtcaa ggcggctaat ggggcgtccg
     1741 acgaatttca aattcatctg ttaacttttt ccttcttggt gctacacgac cagggttcgt
     1801 agacgcgaac tgaggtaatc cactagcata acgagcgcaa tcagaacaag cacgatggcc
     1861 gctgcttcct gatattgaaa ggtacgaaag ctagtgttca tcaagtagcc aatgccgccg
     1921 gctccaatca aaccaaggac ggaggccgct cggatgttgg tttccaaacg atacagtgta
     1981 aacgagatca agtccggcag gatctgcggt aaaacggcaa acgagagaat ctgcgtatag
     2041 ccggcgccgg tggcgcgaat cgcctcaacc acgccctcgt cgatattttc cacggactcg
     2101 gacaacagtt tacccagaac cgtggccgtg tgaatcgcca aggccaaggt tccggcaaaa
     2161 gcgccgagac ccaccgcggc gacgaacaag agacccaacg ccaaatcggg taccgtgcgc
     2221 agcagattca aaacagtgcg aacactatgg taaaccagcg gatggggcgt ggtattgcgc
     2281 gccgcaagaa accccaacgg aaacgccaca atcaacgccg tgaacgtccc tgccagcgcc
     2341 atttgcacgg tctccaaagt tgccccaacc acggtctgtg gaaatatgtt gttccaccaa
     2401 cgctgcttga tccgcgcgct cgcctcgggc aagggcagcg gcagcaagag ctcgcgccac
     2461 gatagttctt ccgggaaata gtagtagttt gcatccgtca gcttagaaaa gtccggcggc
     2521 aacatgcgcg caagagtctg ggctatttgt ggaatgcctt caagcaattc tcccactctg
     2581 atcttcgcgc cctgaaaagc ccaggccagg acaagtagaa aaaccaccgt gaatataaaa
     2641 gaccggagct tatgccttgg tgctggcggc gcctcaagaa agatgtccgg actgtcggtt
     2701 gcttgaagtc gttgaaccaa ggttctcccc ttccgctcgg cgagaaacag ttaactgcta
     2761 agccacagcg ttctcagcac accataaagc accgcaacgt actaaaccag actcggccgt
     2821 gactgcgggg tcactgggaa accctgtcga tgtagatacc gcaacgaaga tctgccggcg
     2881 tttgtcacca tggggtcgct tgaggtacat tctacacgtt catgggcaaa gcctcatatg
     2941 cttgcacgcg ctcgattgcc taacctcaac gtcctagaca agcgcgcctc agattatcgg
     3001 tttctcgcgc gcgtcgaatc tgagtctctt gagcagcctg taacaagatg tcagccagct
     3061 gctaggtcat cattcagtga caacagtaca taatcaagag cgcagcgggc gcggactcta
     3121 gtggtgcaca acgatttctc tctccgattt tggggagtgc gaggcagtta tgcggtgtcg
     3181 gatcgggacg cgctgaaata cggcggcaat acttcttgcg tcgaggtggt tgcgggaaag
     3241 caccaaatca ttttcgatgc cggcaccggc atcatcggac tcggtaaaga gcttgtcaaa
     3301 aacaatctgg gtccgctaag cttaaatctc ttcctgagcc acacacatca cgaccatctt
     3361 tcgggctttt atttttttga tccgctcttc gataaacgca accggatcaa cattttcggc
     3421 ccgggttcgg gaaaacgatc attggctgaa actttgcgca tggcgatgga gcctgcgctt
     3481 tttcccatca ggctcgatgc tcttgatgcg aagaaatcca tctggtcact ggttggcggc
     3541 gaaactatcg cattgcgcga caaccgccgg gccccccgga taaccaaggc cgttagtctc
     3601 agggataagg atagggagcg accgatcatt attacccatg gcagtcccgc ccaccccaac
     3661 ggggtgattt tgtatcgggt tcattttcag ggtaaaagtc tggtttacgc cacggatcta
     3721 gaggagacag cgggcggttc tccagacgta atcgagttcg cgcgcgatac cgacctattg
     3781 atacatgacg cccaatattt agagtcggaa tacatgtcaa cggcaaatcc acgcagaggc
     3841 tggggacaca gcacggttga atcggcgacc gaagttgcca aaaaagccgg cgttaaacaa
     3901 cttgtgttgt ttcatcacga accgacgcat gatgaccgct gtatcgatca gatactgaaa
     3961 ttagctcgac ggcgctttcc tgggacgact gttgcggcgg aaggcatggt catgcgtctc
     4021 tgaagcaaga gcgattgcag ccaatcgatg caggtcttct cccggtttag ctactctgtc
     4081 gcgggttctc ggcgaatgtt caagttcagc agacttcaac gctggctgtt catgggacga
     4141 ctgcaatcga gtaagcatta gagctggctc cttgtaaagc gaagccggag cttgtcccgc
     4201 tcggagttcg ttcgatactt ccgtcagttc ttttctcggc ataagatgca gtggttaccc
     4261 tgaaacggaa gtcggagagg cctccgtttg tgctgcggca gacgttcacc tgttgttgtt
     4321 cgaccaattt accggaagcg gagtgaaagc atgggttggg atctctcaag ctactttcct
     4381 gaattcaatg ggccggaaat gcgtcggttc aaagacgagc tggcccgcga tatcgcttca
     4441 ctgaaagccg aagcggctgc gctgccgccg t
//
LOCUS       DASUTG010000185         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1660404, whole genome shotgun sequence.
ACCESSION   DASUTG010000185 DASUTG010000000
VERSION     DASUTG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660404"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1795
                     /locus_tag="VF089_04155"
     CDS             131..1795
                     /locus_tag="VF089_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173183.1"
                     /translation="MTHDLLIKNGTVVDGSGNPAYQADVAVDGDKIAAIGKNLGSGKK
                     EIDAKGQVVAPGFIDSHTHMDAFIVQYPNGNPVVNYGVTSIVIGDCGASCAPVPPKPE
                     PRQVLVQYLRRVLDKYVDDKDWKWNTFAEYLNYLESKVAINVLPLMPHSPVRLTVMGE
                     AAYAREATPEELDQMKKMVREGMELGAIGFSTSPRGGPAIHAGTPSTFATHQEIIELA
                     GVAAEYNGCFQFNGFQMVLQPESGVPEMLTKIKTLQIGNEFRVRPGAKDAGMQSIKYM
                     EEAQKRGQDIYGVVIPYQHIRRFNLDDCFLFNGLPTWEAIKTSSDLKSKLADKEIRRK
                     IEKERIECTGKLEFPEWHGWDRVVFEHVKKPELKKIEGKNVAEIARLTEKAPVDAFFD
                     TWLEDNLNSQLLYHGLANAHPELLAEMIKSDTSLIGTDVGAHLDRFFWHGAPTKILGF
                     WRREKNLFSLEQAVHKLTGFPAKKLRLNRGLVKEGMPADLTVFDPDKIDDLVSKQLPD
                     MIDAQEVKRHPPGMKAVIVNGATVVEEGQCNDVFPGKVRRQQLCIN"
     gene            1885..>2824
                     /locus_tag="VF089_04160"
     CDS             1885..>2824
                     /locus_tag="VF089_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173184.1"
                     /translation="MKTCAIDIHHHYVPKSLLEEAKKRGKHLGVELTEKDGRQSLSFA
                     GGPPFVLHPELPAIDERLKMMQDSKLAMAALEAHTATLGYRLTGEQGENWCTVYNEGI
                     KGLIDSHPDRFVGMASVPLQDPNRAAIVLEHAVRDLKFKGGYIGTNVNGNYYGTPDFD
                     PFWAKAQELDVMIVMHPEDVAGADKMNPYGLKLICGNPADSALCFGYLTYSGVFDRFP
                     NLKLCILHGGGFFPYHLGRFDQGWQVRAGARAPQAKMPPSAYLKNIYYDNMIYRLDTI
                     KYLIGLAGLDHIMVGTDYPYDLGDWNAAKKVEELDVS"
BASE COUNT          705 a          747 c          789 g          583 t
ORIGIN      
        1 ccggcgagtc acgggagatg aagaaggatc gtgtaatgcg gcccgacttc gacggacata
       61 gcgggcggtt gacagcctgg gaccttcggt gggataagcg aaatatgttt acaaaggagg
      121 ctacacgttt atgactcatg acttactgat caagaacggc acggtcgtcg atggcagtgg
      181 aaatccggcc tatcaggcag acgtggccgt cgatggcgac aagatcgccg cgatcggcaa
      241 aaatctaggc tccggaaaaa aggagattga cgccaaggga caggtagtgg cgccgggatt
      301 catcgactct catacccata tggatgcctt tatcgttcag tatcccaatg gcaatcccgt
      361 ggttaattac ggcgtaacca gcattgtcat tggtgattgc ggcgcctcgt gcgcaccggt
      421 tccgccgaag cctgaaccgc gccaagtttt agttcagtat ctacgccgcg tacttgataa
      481 gtatgtcgat gataaggatt ggaagtggaa tacattcgcc gagtatctga actacctcga
      541 gagcaaggtc gcgatcaacg tgctgccgct gatgcctcac tctccggttc gcttgaccgt
      601 catgggagaa gcggcttacg cgcgcgaagc cactcctgaa gagctggatc aaatgaaaaa
      661 aatggtccgc gaaggcatgg agttgggcgc catcggtttc tccaccagcc cgcgcggggg
      721 gcccgcgatt cacgccggca cgccgagcac atttgcgacg caccaggaga tcatcgaact
      781 agccggggtc gccgccgaat acaacggctg ctttcaattc aatggattcc aaatggtcct
      841 gcaacctgaa tcgggcgttc cggaaatgct gacgaaaatt aaaactttac agatcggcaa
      901 cgagttccgc gtgcgccccg gcgcaaagga cgcgggcatg cagtcgatca aatacatgga
      961 agaagcgcaa aagcgcggcc aggatattta cggcgttgtc atcccttacc agcatattcg
     1021 ccggtttaat ctcgatgatt gttttctttt caacggcttg ccaacctggg aagcgatcaa
     1081 aacttcgagc gatttaaagt ccaagctggc cgataaagag attcggagga aaatcgaaaa
     1141 agagcggatc gaatgcacag gcaaactcga atttcccgaa tggcatggtt gggatcgggt
     1201 ggtattcgag cacgtgaaaa agccggagct gaaaaaaatc gaaggtaaga acgtggcaga
     1261 gatcgcgcgc ctgaccgaga aagcgccagt ggacgcgttt ttcgatacct ggctcgaaga
     1321 caatctaaat tcgcaactcc tgtaccacgg tctagccaat gcgcatcccg agctgttggc
     1381 ggaaatgatc aaatccgaca ccagcctgat cggcacagac gtcggagcgc atctcgatcg
     1441 ctttttctgg cacggcgcgc cgacaaagat tcttggcttc tggcgccgcg agaaaaacct
     1501 gttcagcctc gaacaagcgg tccataagct cactggcttt ccagctaaga aacttcggct
     1561 caatcgcggc ttggtcaaag aagggatgcc cgcggatttg acggttttcg acccggataa
     1621 aatcgatgat ctcgtcagca agcagttgcc cgatatgatc gacgcccagg aagtcaagag
     1681 acacccgccg ggaatgaagg cagtgatcgt gaacggcgct acggtggtgg aagaaggtca
     1741 gtgcaatgac gtcttccccg gtaaagtccg gcgtcagcag ctttgcatca attagacatt
     1801 cctcttgcaa tatcgtgcct gatcgtgtgg cgttggctta acggccaacg ccacatttag
     1861 tttcacagaa aaggattatt caaaatgaaa acctgcgcta tcgatatcca tcatcattac
     1921 gtacccaaaa gtctgctgga ggaagccaaa aagcgcggca agcatctcgg cgtcgaactg
     1981 acggaaaaag acgggcggca gtcgttgtca tttgccggcg gcccgccttt tgtgcttcac
     2041 cctgaacttc cggcgatcga cgaacggctg aagatgatgc aggactcgaa gctcgccatg
     2101 gctgcgttgg aagcccatac agcgaccctg ggttatcgcc tcaccggcga gcaaggcgaa
     2161 aactggtgca ccgtttacaa cgagggaatc aaaggtctga tcgatagcca cccggatcgg
     2221 tttgtcggca tggcctcggt gccgctacag gacccgaacc gcgccgccat agttttggaa
     2281 cacgcggtgc gcgatttgaa gtttaagggc ggttacatag gcaccaacgt caacggcaac
     2341 tattacggca cgccggattt cgacccgttc tgggccaaag cgcaagagct cgatgtcatg
     2401 atcgtgatgc atccagagga cgtggccggc gccgataaga tgaatcccta tggtttgaaa
     2461 ctgatttgcg gcaatcccgc agacagcgcc ctgtgcttcg gctatctgac atacagcggc
     2521 gttttcgacc gctttcccaa tctcaagctc tgcatactcc acggcggcgg attcttcccc
     2581 tatcatctcg ggcgcttcga tcagggctgg caagtccgcg ccggcgcgcg cgcaccgcag
     2641 gccaaaatgc cgccaagcgc ttatctaaag aacatctatt acgacaacat gatctatcgg
     2701 ctggatacca tcaaatatct gatcggcttg gcgggtctgg accatattat ggtcggcacg
     2761 gattacccat atgatctggg ggattggaac gccgcaaaaa aggttgagga actggacgtc
     2821 tccg
//
LOCUS       DASUTG010000186         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_623410, whole genome shotgun sequence.
ACCESSION   DASUTG010000186 DASUTG010000000
VERSION     DASUTG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623410"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2857)
                     /locus_tag="VF089_04165"
     CDS             complement(<1..>2857)
                     /locus_tag="VF089_04165"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173185.1"
                     /translation="LILEEIRRRLGYLDGVGLEYLTLDRQSRTLSGGELERVDLTTAI
                     GSSLVNTLYVLDEPSIGLHPRDSKRLVEILHRLKANQNTVVVVEHDPEIIKECDHIID
                     MGPKAGQQGGNIMFAGSYQALLKDEKSLTAAYLSQRKTIPLPPHSRKPLSRRSIKISG
                     ACANNLKNIDVEIPLGLMVCLTGVSGSGKSTLVDEVLYRNLKKLKESPSAKVTQCAAI
                     SGAEKISEVVLVDQSPVGTTPRSNPATYMKAFDGIRKVFAATELSRLRGYAPSTFSFN
                     VEGGRCETCRGEGFEKVEMQFLSDVYTSCPECHGSRFRQEVLEVNYRGKNIRQILDLT
                     VAEALDFFEDTAEIKNALAPLQAVGLEYVCLGQPLTTLSGGESQRLKLAWHMSKARKA
                     GTLFIFDEPTTGLHFHDIERLLRAFNELLDQGHSIVIIEHNLEVIKCADHIIDLGPEG
                     GDKGGEIVAIGTPEEIAAVEASHTGRYLGPCLKRSKVSRFSPTLVNRSTVPKFNGSTA
                     EDNAIAIVGAKEHNLKNISIDIPRDRFIVFTGLSGSGKSSLAFDIVYAEGQRRYIDSL
                     SAYARQFLEVMARPNVDFISGIPPTVAIEQRLSQGGNKSTVATVTEIYHYLRLLYSKI
                     GKQHCVQCGRRIQSLTRSQILDRIGRSYRGKEIMILSPIVRGRKGFHKEVIAGARKLG
                     YRRARIDGELMDLRAPELSGGLERFQEHNIDIVIGETKVGGREIEALLDQGLRLGNGV
                     IHLISERGQQVFNQRLFCLECGVGYEPLDPRFFSFNSRQGACQECSGLGFSWDFDPEL
                     IFTDPRQPLNHAIAGISVGSSTGDADLQRALQRFLHELKDKQRVDIEQPLAKLPKKLQ
                     QDILYGGNGRSGCAGLVPFLKQLQQDAADELHSVLDELMTETPCPACSGRRLNPRAQA
                     VKLDGKAIWQVTSFSVDQANDYFAKLNLSFSDNGNSER"
BASE COUNT          599 a          797 c          820 g          641 t
ORIGIN      
        1 ctcgttcgct gttgccgtta tcggaaaacg acaggttgag cttggcaaaa tagtcatttg
       61 cctgatccac ggaaaacgag gtgacctgcc agatcgcttt gccatcgagc ttcaccgcct
      121 gcgcgcgggg attgagccgg cgaccggagc aggcgggaca cggcgtctcg gtcatcagct
      181 catcgagcac gctgtgcaac tcatcggcgg cgtcctgctg cagttgtttg aggaagggca
      241 ccaacccggc gcagcccgag cggccgttgc cgccatagag gatgtcctgc tgcagtttct
      301 tcggtagctt ggccagtggc tgctcgatgt ccacccgttg tttgtctttg agctcatgca
      361 gaaatctctg cagcgccctt tgcaggtcgg cgtcaccggt cgatgatccg acagaaatgc
      421 cggcgatcgc atgatttagc ggttggcgcg gatcggtgaa aatcaattcg ggatcaaaat
      481 cccagctaaa gccaagcccg ctgcactcct gacatgctcc ctgacggctg ttgaatgaga
      541 aaaaccgcgg atccagcggc tcataaccga cgccgcattc aaggcaaaat aacctctggt
      601 taaaaacctg ctggccgcgc tcggagatga gatggatgac gccgttgccc agacgcagtc
      661 cttgatcgag cagcgcctcg atttcgcgcc cgccgacttt ggtctcgccg atgacgatgt
      721 cgatgttatg ttcctgaaag cgctcgaggc cgccgctcag ctcgggcgct cgcagatcca
      781 tcaactcgcc gtcgatgcgt gccctgcgat agccgagctt tctggcgccg gcgatcactt
      841 ctttgtgaaa tcctttgcgg ccgcgcacga tcggacttag gatcatgatt tccttgccgc
      901 gataggagcg gccgatgcga tcgagaatct ggctgcgggt taacgattga atacgccggc
      961 cgcattgcac gcaatgctgt ttgccgatct tcgaatagag cagtcgcaaa tagtgataaa
     1021 tctccgtgac tgtcgcgacg gtggacttgt tgcccccctg gctaagtctt tgctcgattg
     1081 cgacggtggg cggtatgccc gagataaaat cgacgttggg ccgcgccatc acttccaaaa
     1141 actgccgcgc gtaagcggac aggctgtcga tgtagcggcg ctggccttcg gcatagacaa
     1201 tgtcaaacgc cagcgaagat tttcccgagc cgctcaagcc ggtaaaaacg atgaagcgat
     1261 cgcggggaat gtcgatgctg atatttttaa gattgtgctc cttcgccccg actatggcga
     1321 tggcattgtc ctcggccgtt gaaccgttga actttggaac tgttgaacga tttacgagcg
     1381 tcgggctaaa ccgtgaaact ttgcttctct tcaagcacgg acctaggtaa cggcccgtgt
     1441 gcgaggcttc gaccgcggcg atttcttccg gggtgccgat agcgacaatc tctccgcctt
     1501 tatcaccgcc ctcgggcccg agatcgatga tgtgatcggc gcacttgatg acctccaggt
     1561 tgtgctcgat gatgacaatg gaatggccct ggtcgaggag ctcgttgaag gcgcgcagca
     1621 gcctttcgat atcatggaag tgcaacccgg tggtgggctc gtcgaagata aacaaggtgc
     1681 ccgctttcct cgccttggac atgtgccatg ccagctttaa tctttgtgac tcgccaccgg
     1741 agagagtagt cagcggttgt cccaggcaca cgtattccag gccgaccgcc tgtagcggag
     1801 ctaaagcgtt tttgatctcg gcagtatctt caaagaaatc caaagcctcg gcgacggtaa
     1861 ggtcaagaat ctgccggata tttttaccgc gataattgac ttctaaaacc tcttgtcgga
     1921 agcggctgcc gtggcattcg gggcaggaag tgtaaacatc ggagagaaac tgcatttcga
     1981 ccttctcaaa cccttcgccc cggcaggttt cgcaacgtcc cccttcgacg ttgaacgaga
     2041 aggtcgaagg cgcgtaaccg cgcaagcgcg acaactcggt cgcggcgaat actttgcgga
     2101 tgccgtcgaa ggctttcatg taagtcgccg ggttggaacg cggcgtggtg ccaaccggag
     2161 attgatcgac cagtacgact tcggaaatct tctcggcgcc gctgatggcc gcgcactgcg
     2221 tcaccttggc agacggcgat tctttgagct tcttcagatt ccgatagaga acctcgtcga
     2281 ccagagtcga cttacccgaa cccgaaacgc ctgtgagaca gaccatcaac ccgagcggaa
     2341 tctcaacatc gatgtttttc agattattcg cacaggcgcc gctgatcttg atcgaccggc
     2401 gcgacaatgg ctttcggcta tgcggcggaa gaggaatggt tttgcgctgg ctcagataag
     2461 ccgctgtaag ggatttctcg tctttcagca gcgcctgata ggatccggca aacatgatat
     2521 tgccgccttg ctgccccgct ttcggaccca tatcgatgat atggtcgcac tctttgatga
     2581 tttcgggatc atgctcgacg acgaccactg tattttgatt ggccttgagc cgatgaagga
     2641 tttctaccaa ccgcttgcta tcgcgcggat gcaacccgat cgacggctcg tcgagaacat
     2701 agagcgtatt gaccagagat gagccgatcg cggtggtgag gtcgacgcgc tcgagctcgc
     2761 cgccggagag tgtgcgcgat tggcgatcga gagtcaggta ttccaggccg acaccatcca
     2821 gataacccag gcgtcggcgg atttcttcca atatgag
//
LOCUS       DASUTG010000187         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1067882, whole genome shotgun sequence.
ACCESSION   DASUTG010000187 DASUTG010000000
VERSION     DASUTG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067882"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VF089_04170"
     CDS             <1..1252
                     /locus_tag="VF089_04170"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX6173186.1"
                     /translation="GTRLHPLTKLRAKPAVPLAGKYRLIDIPISNCINSEIQRIYVLT
                     QFNSASLNRHITRTYGMAGFQQGFVEVLAAQQTPENPNWFEGTADAVRKYCWLFAEWD
                     VDQYLILSGDHLYRMNYADFIRRHRETQADVTLAVIPVAERGASDFGLMKIDTQGRVQ
                     SFSEKPKGEALKLMQVDTRALGLPADKASELPYIASMGIYVFERQVLFDLLKESPDRT
                     DFGKEILPAALKNYNVQAYLFKDYWEDIGTIEAFYNANLALTQQPNPPFSFYDEKAPI
                     YTRSRYLPPTKLLDCNVVESMVGEGCILRECTVNRSVVGIRSRIETGCVIDNVLLMGS
                     DFYESPQERLQTVESGKVPLGIGANTTIQRAIIDKNARIGRNVRMVNQDHVQDGTRED
                     LGFVIRNGIIVVIKNATIPDGTII"
     gene            1509..3206
                     /locus_tag="VF089_04175"
     CDS             1509..3206
                     /locus_tag="VF089_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6173187.1"
                     /translation="MTHYSFPQLLRHDFAGYNIPKFRQDLLAGLTVAAVALPLALAFG
                     VASGASASAGLITAILAGLIIGGLSGAPYQISGPTGAMSAVLIVLAQKHGLPGIWFAG
                     MLAGILLLVIGFARLGRFIAFVPAPVIAGFTSGIACIIAIGQIDNLLGVQSGPASSAA
                     LKLLGYFNAAAIPDWHALTLGTVVIVTMVFWPKPWSARFPASLLAIIVATVMNAVLGW
                     PVGVIGEIPKTLLLDQRLSFELIPWSHVQEFIAPALAITALGAVESLLCGAVASNMTG
                     IRLQANQELIAQGIGNAIIPFFGGVPATAAIARSSVGIKSGGQTRMVSIVHAVGLLAS
                     MFLLGPIMETIPLAALAGVLMVTAWHMNEWEAIHYIFSHRFKTAMITFLVTMVATVVL
                     DLTEAILLGAFLSGAIFLNQSASIDIDVQEVDPEKLRKRGIENAGSCQHVRVAYLTGP
                     LFFAATSNFNEAFARFESTHALILSMRGVPLIDTSGLQAMTALFHKLNKQGITLSVAA
                     AHEDVLRMMERGGLISLIGSENVFWSSDQAIVALESRGCRYCGNPKPAIARPQVVAI"
     gene            3593..3820
                     /locus_tag="VF089_04180"
     CDS             3593..3820
                     /locus_tag="VF089_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173188.1"
                     /translation="MADRISKLPRSKKIPPIDVVRARLRNHVEVVLASNNEVLIRMVE
                     RFFTETIDRLIQERPKGEVVPIKPENPTTDK"
BASE COUNT          903 a         1106 c         1135 g          985 t
ORIGIN      
        1 cggaacccgg ctgcacccct taactaagct tcgcgctaaa cccgccgttc ctcttgccgg
       61 caaatatcgc ttgatcgaca tccccatcag caactgcatt aattcggaga tccagcggat
      121 ttatgtcctt acgcagttca attcggcctc gctcaaccgg catatcacgc gaacctacgg
      181 catggccggt ttccagcagg ggttcgttga agtcctggcc gcccagcaga cgccggaaaa
      241 cccaaactgg tttgaaggaa ccgccgacgc ggtgcggaag tattgctggt tatttgccga
      301 gtgggatgtc gatcaatacc tgattctatc gggtgatcat ctttatcgga tgaattacgc
      361 ggacttcatt cgtcgccatc gcgaaactca agccgacgtg actctagccg ttatacctgt
      421 cgcggaacgc ggggcgtcgg atttcggttt aatgaagatc gatacgcaag gacgtgtgca
      481 gtctttcagt gaaaagccca aaggagaggc gctcaagttg atgcaggtcg atacccgcgc
      541 gctgggcctc ccggcggata aagccagcga gcttccctat atcgcttcaa tgggtatcta
      601 tgtgttcgaa aggcaggttc tgttcgatct tttgaaagaa tcccctgatc gaacggactt
      661 cggcaaggaa atccttccgg ctgcgctaaa aaactacaat gtgcaggcgt atctctttaa
      721 agactattgg gaagatattg gaaccatcga agctttttac aacgctaatc tggcgctcac
      781 gcagcagccc aatccgccgt tcagctttta cgatgaaaaa gcgccgatct atactcgctc
      841 gcgctatctc ccgccgacca agttgctcga ttgcaacgtt gttgagtcga tggtcggtga
      901 gggctgtatt cttcgggaat gtaccgtaaa tcgctccgtc gtaggaattc gatcgcgcat
      961 tgaaacgggc tgcgtaatcg ataatgtttt gctgatggga tcagatttct acgaatcccc
     1021 gcaagaacgg ttgcagaccg tggagagtgg aaaggttccg ttggggatcg gagcaaacac
     1081 gaccatccaa cgagccatca tcgataagaa cgcccgcata ggccggaacg tccggatggt
     1141 caatcaggat cacgttcagg atggtactcg tgaggatctt ggctttgtaa ttcgcaacgg
     1201 cattattgtt gtcatcaaga acgctacgat ccctgatggc acaattatct aaccggcaca
     1261 gatccatgtc gaagatttcc taacaccaca agcgctgatt ctcttgcgct gttgactgca
     1321 agactcgggc gggccaattc gcggccgggc ttgatcagct acgctggcgc gcttataatt
     1381 acggccacgt aaacggctag cgactcaccc gatcgctcac ccattcaaca acataaccgc
     1441 tcctgtgtcg catcgcgaga ctctgttgcg gagtcgggct ccgcaacagg tcgtttaagg
     1501 catcttgtat gacgcactat tcattccctc aactcctcag gcatgatttc gccggctata
     1561 acattccaaa attccggcag gatctacttg ccggattgac agttgccgca gtcgcattgc
     1621 cgttggcgct cgccttcggt gtcgcctcgg gtgcgagtgc cagcgccggt ttgatcacag
     1681 ccatattggc agggttaatc attggcgggc tttcaggggc tccttatcag atatcagggc
     1741 cgacgggcgc gatgagcgca gtgctgattg tactggcgca aaagcatggt ttgcctggaa
     1801 tatggtttgc cgggatgctg gccggtatac ttttgttggt catcggtttt gcccggttag
     1861 gccgctttat cgcctttgtt cccgcccccg tcatcgccgg ctttacctcc ggcatcgcgt
     1921 gcatcattgc gatcggtcag atcgataatc ttctcggggt gcaatcaggc ccggcaagct
     1981 cggcggcttt aaagctattg ggttacttca acgctgcggc gattccggat tggcatgctc
     2041 ttaccctcgg caccgtggtg attgttacga tggtcttctg gccaaagcca tggagcgccc
     2101 gctttcccgc gtcactccta gccatcatcg tcgcgacagt tatgaatgcg gtgctcggct
     2161 ggcccgtcgg agtgataggc gaaatcccga aaaccctgtt gctcgatcaa cgcttgagct
     2221 tcgagttgat tccgtggagt catgtccaag agttcatcgc cccggctctg gcgattaccg
     2281 cattgggagc ggtggagagt ctgctctgcg gcgcagtggc gagcaacatg accggaatcc
     2341 gtttgcaggc taatcaggaa ttgattgcgc agggcatcgg caatgcgatc atcccatttt
     2401 tcggcggcgt gccggcaacg gccgccatcg cccgcagcag cgttggtatt aagtccggcg
     2461 gtcagacccg aatggtcagc attgtacacg cagtcggcct actggcctct atgtttttgc
     2521 tcggacccat catggaaacg attccgctcg ccgcccttgc cggagtcttg atggtcacgg
     2581 catggcacat gaacgaatgg gaagcgattc attatatttt tagtcatcgt ttcaaaaccg
     2641 ccatgattac gttcctggtg acaatggtgg cgacggttgt tctggacttg accgaagcga
     2701 ttttactcgg agcgtttttg tcgggcgcga tttttttgaa ccagagcgcg agcatcgata
     2761 tcgatgttca agaagtggac ccggagaagt tgcgcaagcg cggtatcgag aacgccggga
     2821 gctgccagca tgtgcgagtc gcgtatttga ccggccccct gttcttcgcc gccaccagca
     2881 atttcaacga agcctttgcc cgtttcgaaa gcacgcatgc gcttattctc tcgatgcgag
     2941 gcgtgccttt gatcgacacc tccggcctgc aagcgatgac ggcgttattc cataagctca
     3001 acaagcaagg catcaccctt agcgtggccg cggcccatga ggatgtattg cgtatgatgg
     3061 agcgaggcgg gttgatttcg ctcatcggct cggaaaatgt tttctggagc tcggatcaag
     3121 cgatcgtcgc gctggaaagc cgcggctgcc gttattgcgg caacccgaaa ccggctattg
     3181 cccgcccgca agtcgttgcc atctaatcca tgtgagtaag gtttctaaca gtggcggcag
     3241 aattccccga gtcgattccc gtatctttct cataggaaaa cggaagtttt tgggtcgcta
     3301 tgaagtcatg tgaacatttc tggcttcgag actaaaccca ccagttcgat aaccttgcga
     3361 gtcctgatat cctcgaagaa gagcgtcgat gacttcttat ccgcccgtcg atatttcaac
     3421 gctgccccgg agaccaccga gagctaacgg gggctgtgtc cgtaaagaag ttggcagagg
     3481 aacgccgttt ctgagttggt ttacctagaa agcggatccg cgagttcttg aacttctaga
     3541 aaaactcgtc caggttcttc tttcggtagg acggatctga tggaatagag ctatggctga
     3601 ccgaatctcg aagctgcccc gcagtaaaaa gatccctccc atcgatgtcg tccgcgccag
     3661 attgcgcaat cacgtcgaag ttgtcctggc gtcgaataac gaggtgctaa tccggatggt
     3721 tgagagattt tttaccgaaa cgatcgaccg cctgatccag gagcgcccga agggcgaggt
     3781 cgttccgata aagccagaga atccgacgac tgataagtaa gtaaaacgct ggtggcgagc
     3841 atggatccga agaagcttcc acttgtgccg ctcgatccgg caattccgac gaatgcgagc
     3901 gtcgttcggg agttccggtc cgtcttcgga ttgaagtaac gtgatcttac gcgcgcagcg
     3961 ctaatagtca tgcacgcatg cgcacgagcc acgctcacga gcatgaaacc ggtgaaggtg
     4021 atacgttgcg cagttagccg gccgaaagga gaggacgtga cgatggaaat cacaagtggc
     4081 aagttcgtcc tgttcaccca gcccggttga agcagttgcc ttagaacga
//
LOCUS       DASUTG010000188         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_178860, whole genome shotgun sequence.
ACCESSION   DASUTG010000188 DASUTG010000000
VERSION     DASUTG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178860"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VF089_04185"
     CDS             <1..1213
                     /locus_tag="VF089_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6173189.1"
                     /translation="STPEATVIGSARKVNAQYAALANGIRGHVLDYDDAQLATLGSRP
                     YGQLTHPTTPVLAAALALAEKIKATGLELLIAYIAGLEVACRLADAIHPDHYIKGFHP
                     TGTFGAFGETAACAHLLKLDFTHARWALGIAGSLASGVRAHRGTMGKSFNAGHAAENG
                     IVAATLARSGFTASTDVFDDSMGYFAAACHGKVDRKLIKLGAPYFLVNPGVAIKIYPC
                     AGVLHPALDAIIDLAKEHDLRWTDIGRIRVSLGPDAALPLVSTSPRTALEGRFSLPFS
                     AAVALIYRRAGLDQYQEATLLDPKVTAVMKRVDLVRDSRLQSLGNFACKAEVEISLTN
                     GKTHRKRAVIAKGHPKKPLTREEILEKFYQCAGERLPKNRANKFIENLWSIEKVDALA
                     PWIGLLRPRRR"
     gene            1272..1973
                     /locus_tag="VF089_04190"
     CDS             1272..1973
                     /locus_tag="VF089_04190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6173190.1"
                     /translation="MRIVDLSMTVQECDSAPFAPDESYFKLRPIIKWEDKGFVSNVVQ
                     MTVHVGTHIDSPHHFFRDKPSIEQLPLEPMFGRAIILDLTEKGVARAIITPEDLDKAE
                     EKLKQQGIEIPQDGMLFLRTDWPKGHDTTDPQWWNDSPYLNKEAAQWIVAKKPAVVGF
                     DFAQEEKGTDYQSADQILQSAMRVHRTILPRVIFQIENLINLDQIGPSAQVVALPVKW
                     KTESAPARVIALVDN"
     gene            2107..3096
                     /locus_tag="VF089_04195"
     CDS             2107..3096
                     /locus_tag="VF089_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX6173191.1"
                     /translation="MRAVRVHELGGPDVLRYEDVPAPQPKAGEVLVRIAAAGVNYLDI
                     YYRSGFHWGGHHGRALPYIPGAEAAGTVVDLGAGVTDVKVGDRVAYGISNGYGSYAEL
                     HAVPAWHIYKLPPSVAFEDAAAVMLQGMTAHYLTHSTYRIKPKDTVLIHAAAGGTGLL
                     LVQLAKMRGARVFGTVSTDAKAQLAQVAGADRTINYAEQDFADEVRKLTDGKGVNVVY
                     DSVGQATFEKSLDCLAPLGSLIIFGQASGPVPPFDTSILNAKGSLTLARPSLTHNVAS
                     YADVLWRAGDLFNWLAEGKLKVTIGEIYRLSQAADAHRQLESRKSTGKILLVP"
BASE COUNT          712 a          866 c          853 g          669 t
ORIGIN      
        1 ctccacaccc gaagcaaccg tcattggctc cgccaggaag gttaatgcgc agtatgcggc
       61 gctcgccaac ggaattcggg gacacgtgct cgactacgat gatgcccagt tggcgacttt
      121 gggttctcgg ccctacggtc aactcacgca tccaaccaca ccggttctag ctgcggctct
      181 agccttggct gaaaaaatca aggccacggg tttagaattg ttaatcgcct acattgcggg
      241 tcttgaagtg gcgtgccggc ttgccgatgc aattcatccc gatcactaca tcaaaggctt
      301 tcatcccacc gggacattcg gcgcattcgg cgaaaccgcc gcttgcgcgc acctgctgaa
      361 actagatttt acccatgccc gctgggctct gggcatcgcc ggaagcctgg cctccggtgt
      421 gcgcgctcat cgcgggacca tgggcaaatc tttcaatgcc gggcatgcag ccgagaatgg
      481 tatcgttgcc gcaacgttgg cccgatccgg ctttaccgct tccactgatg tattcgacga
      541 ctccatgggt tacttcgctg cggcgtgtca tggcaaggta gatcggaaac tgatcaagct
      601 cggagcgccg tattttcttg ttaacccggg ggttgcgatt aagatctatc cttgcgccgg
      661 tgtattacat ccggcgctcg acgcaatcat cgatctagcc aaagaacacg atttgcgatg
      721 gaccgacatc ggaagaattc gggtcagtct cggtcctgat gcggctttgc ctttagtgtc
      781 taccagcccc agaactgctc ttgaggggag gttcagtcta cccttctccg ccgctgtggc
      841 tttgatctat cgccgagccg gtctcgacca atatcaagag gccactcttc ttgatccaaa
      901 ggtcactgcc gttatgaagc gagtcgacct tgtgcgagat tcaaggctcc agtcgcttgg
      961 caatttcgct tgcaaggccg aggttgaaat ttcgttaact aacgggaaga cccaccggaa
     1021 acgcgccgtc atagcgaaag ggcatccgaa gaaacctctg acgcgagaag aaattctgga
     1081 gaaattttat cagtgtgccg gcgagcgatt accgaaaaat cgcgccaaca agtttatcga
     1141 gaatctctgg tccatcgaaa aagttgatgc gctggcgcca tggattggat tgttgcggcc
     1201 caggcgccgt tgactttgaa cgtgtatacg aagaaaaatg aaacaacact gacgttggaa
     1261 aggatcatac catgcgcatc gtcgatctca gcatgaccgt ccaagaatgc gactcggcac
     1321 cgtttgctcc cgatgaaagc tattttaagc tcagaccgat cattaaatgg gaggataaag
     1381 ggtttgtttc caacgtcgtg cagatgaccg ttcacgtcgg tactcacatc gactcgccac
     1441 atcatttttt tcgcgacaag ccttctatag agcaacttcc gctcgagccg atgttcggcc
     1501 gggcgatcat actggatcta accgagaagg gcgttgcccg cgcgatcatc acgccggagg
     1561 acttagacaa ggccgaggag aagctcaagc aacaaggcat cgagattccg caagacggca
     1621 tgctttttct taggaccgac tggcccaaag gccacgacac gacggatcct caatggtgga
     1681 acgattcgcc gtatttaaac aaggaggcgg cccaatggat tgttgcgaag aagcccgcgg
     1741 ttgtcggttt tgattttgcg caggaagaaa aagggaccga ttatcaaagc gccgatcaaa
     1801 ttctccagag cgccatgcgc gtgcaccgga ccatccttcc cagagtgatc tttcaaatcg
     1861 aaaatctgat caacctcgat cagatcggcc ccagtgctca agtcgtggct ctgccggtca
     1921 agtggaaaac cgaatcggca ccggcgcggg tcatcgccct agtggacaac tagcagggtg
     1981 ctgaaaaaag cctctgcagg ctgctcaaaa tgcctgatag gcgaaggagt aatctcagta
     2041 cgtcggaagc gaggagaatg agcgcaacaa agcctgtgcg gcttttcaag tagcctggga
     2101 tgactgatga gagcagtccg tgtacatgag ctgggcggcc cggacgtgct ccgctacgag
     2161 gacgttccgg cgccgcaacc aaaagccggc gaggttttgg ttagaatcgc tgccgccggg
     2221 gtcaactacc tggacatcta ttatcgttca ggctttcact ggggcggcca tcatggccgc
     2281 gcgcttccct atattcccgg cgctgaggcg gccgggaccg tcgtcgactt aggcgccggc
     2341 gtgaccgatg tcaaagtcgg tgatcgcgtt gcctacggca tatcgaatgg gtatggctcg
     2401 tacgccgaac tgcacgcggt gcccgcctgg catatttaca agcttccgcc ctcggttgcc
     2461 ttcgaggatg cggcagccgt catgctgcaa gggatgacgg ctcattacct cacccacagc
     2521 acttatcgga taaaacccaa agacactgtt ctcatccacg ccgccgccgg tggaaccgga
     2581 ttgttgttag tccaactagc caagatgcgc ggcgcgcggg tctttgggac ggtctcgacc
     2641 gatgccaaag cgcagctcgc tcaagttgcc ggcgccgatc ggacgatcaa ctacgcggaa
     2701 caggatttcg ccgacgaagt tagaaagttg accgacggca aaggcgttaa tgtcgtctat
     2761 gattcagttg gccaggcgac atttgaaaaa agcctggact gccttgcccc attgggcagt
     2821 ctgatcattt tcggtcaagc cagcggcccg gtgccgccct tcgatacttc gattctaaac
     2881 gccaagggat cgctgacgtt agcccgtccg agcctgacac acaacgtcgc cagctatgcc
     2941 gacgtgcttt ggcgcgccgg cgatctgttt aactggctcg cggaaggaaa gcttaaagtt
     3001 acgattggag aaatctaccg gctttcccaa gccgcggatg cgcaccgcca attggagtca
     3061 cgaaaatcca ccggcaagat tctcctagtc ccttagaaaa
//
LOCUS       DASUTG010000189         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_178898, whole genome shotgun sequence.
ACCESSION   DASUTG010000189 DASUTG010000000
VERSION     DASUTG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178898"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF089_04200"
     CDS             complement(<1..255)
                     /locus_tag="VF089_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX6173192.1"
                     /translation="MAHQDRLNIAVFIDFDNIEIGVKSTLGENFDIGAILDALKERGE
                     VVTKIAYADWTRAGEYSRHLTQHAIRLVQRNLTPGGDKNGA"
     gene            787..1065
                     /locus_tag="VF089_04205"
     CDS             787..1065
                     /locus_tag="VF089_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX6173193.1"
                     /translation="MIKTFKCADTEALSKGERVKRFVNIAAVARRKLRQLEIADRLED
                     LRVPPGNRLEALKGDRAGQHSIRVNDQYRVCFRWTDADAEDVEIVDYH"
     gene            1078..1386
                     /locus_tag="VF089_04210"
     CDS             1078..1386
                     /locus_tag="VF089_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX6173194.1"
                     /translation="MRKLKPITPGELLLEEFLRPMGISRYRLAKEIGVPAQRIGDIVA
                     GKRSITADTDLRLCRFFGLSNGYWLRAQAAYDTEVAERALGAKLRKIKPWAGSVEVAP
                     "
     gene            1454..1693
                     /locus_tag="VF089_04215"
     CDS             1454..1693
                     /locus_tag="VF089_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173195.1"
                     /translation="MVEKGFVTEQDFREFTFTNAAMLHMRNNPGFVKGTVVEQAVAEE
                     LALEIARRTGELSIAFLIEENPGVAFDGSGNAGAR"
     gene            complement(1932..2939)
                     /locus_tag="VF089_04220"
     CDS             complement(1932..2939)
                     /locus_tag="VF089_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173196.1"
                     /translation="MKIFHTLLRTQKIACLFALLLAWPPASVFGAAAPTSMRILYPSF
                     AGSWGTTWIAKEAGYFLDERLDVELIRVGGSSRMVAAMIGGNASIIQAGAIAAVQANT
                     AGGDVVIIGATGAVSSFRLMARPEIKNVSDLKGKRAGITTFGSTSDQVIRIALKKSNL
                     EPNKDVALLSLGAQPEVFAALQSGAVQAGAFTFPLYAAAAKLGMRELINFGALGVEDI
                     TGTVITTRSFIAKERDTALRFLRAFTRGMQRYRTDKEFSKKVLGKYGKITDEEALEGT
                     WQDYAPTLQTTPRPSLKAIQFMIENQYPDKKPLPKPEQFVDLTLVDQLEKSGFIDSVN
                     R"
     gene            complement(3772..4275)
                     /locus_tag="VF089_04225"
     CDS             complement(3772..4275)
                     /locus_tag="VF089_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX6173197.1"
                     /translation="MSFLTDEQKDLANEIAALLTERGETVCVAESTTGGLVSAALLWV
                     AGASKYYAGGSVLYTLGSRTALAGVPASMFENYRGTTPEMIAAIADAMRQRLGATWCI
                     AESGLAGPTRGRSGLPPGRTTICVCGPVTRVNVQQTGLPDREPNMIAFTTLTLRYLRD
                     AIKAAEH"
     gene            4733..>5105
                     /locus_tag="VF089_04230"
     CDS             4733..>5105
                     /locus_tag="VF089_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX6173198.1"
                     /translation="MENKRHAPRKFFAETREHWPIMSVYERFEQIVAIVLSLVISVVI
                     ALALVQLVIRIVPLLLSGAIDPLEHDVFQALFGMIMTLLIAMEFKHSIVRVALRRSSI
                     IQVKTVVLIALIALSRKFVILD"
BASE COUNT         1085 a         1395 c         1506 g         1119 t
ORIGIN      
        1 cgccccgttc ttgtcgccgc cgggcgtcag attgcgctgc accagccgga tggcgtgctg
       61 cgtgagatgg cggctgtact cgcccgcgcg cgtccagtcg gcataggcga tcttggtgac
      121 cacctcaccc cgctccttca atgcgtcgag gatagcgccg atgtcgaaat tctctccgag
      181 cgtcgattta acgccgattt cgatattgtc gaagtcgatg aagacggcga tgttgagacg
      241 gtcctgatgg gccaaatgta atcctttcaa ctttctaatt tttatgccga gaggggcgcc
      301 ggcgcctcgg ggggcgggac gatcacccgc gtaaattggt cacgctgcat gatctcgcgt
      361 atgcatgaga aattcaaacc agcttttgtt ctatccgagc agtaacgccg gaccggagag
      421 ttgtcaattg agttttctga tcgggcttca tcaccgatca atttttttga gagttcacgt
      481 tcaccgatgc cgtaatgttg cacacgcgca acaatccggg ctttttcaag gataccgttg
      541 tcgagcaagc ggttgccgag gagttgggcc tggaaagagc atcgcccgct gcgaccgcgt
      601 agataactta ctaggcgtag gggccgagcg aggggaagaa aaacggcaac aggcaattga
      661 taagaagagt aaagacgtga cccgacggcc cgcggctcag cctcgaactc cgttgggccg
      721 ctcaagctcg acccacaaca atagtcatgg ttgaggttaa tcacggcctg cgttactatc
      781 ctactggtga tcaagacatt caagtgcgcc gacacggagg ctctctcaaa gggcgagcgg
      841 gtcaaacgct ttgtaaacat tgctgccgtt gcgcgacgca agctgcgaca gcttgagatc
      901 gcggaccggc tggaggatct tcgggttcct ccaggcaatc ggttggaggc actgaaaggc
      961 gatcgcgctg ggcagcacag cattcgtgtg aatgatcagt accgcgtttg tttccgttgg
     1021 acggacgcag acgctgaaga tgtcgaaatc gtggactatc actgacggag gatgattatg
     1081 cgtaaactca aacccataac acccggcgag ctgttgctgg aagaatttct gcgaccgatg
     1141 gggattagtc gataccggct ggcaaaggag atcggcgtgc cggcgcagcg tatcggcgat
     1201 atcgtggcgg gcaaacgttc catcactgct gacacggact tgcgcctatg ccgtttcttc
     1261 ggcctttcta acggctactg gctgcgtgcc caagcagcat atgacacgga ggttgcggaa
     1321 cgagctttgg gcgctaagct gcggaagatt aaaccctggg ccggctcagt ggaagtcgcg
     1381 ccctagccgg cgagacaagg gctcaggatg agcccagacc tgacccgcgg atgtcatacc
     1441 cgaagcgttt gagatggttg agaagggctt cgtcaccgag caggattttc gcgagttcac
     1501 gttcaccaat gccgccatgc tgcacatgcg caacaatccg ggcttcgtca agggcacagt
     1561 tgtcgagcaa gcggttgccg aggagttggc tctggaaata gcacgccgga ccggagagtt
     1621 gtcaattgcc tttctcatcg aagaaaatcc cggtgttgct tttgatggtt cggggaatgc
     1681 aggagcccgg tgatcggcga gcgatttggc cgtttggccg aggagctggc ccgattgcgg
     1741 ggcgaccagg gaaaagatcg agaattaacc gaagataccc gaagcgggtt caggacctta
     1801 ccacacatcg gtaagcggta gagtcaggat ttaacactgg agcacgccct caacccgatc
     1861 ggttgggatt aggtcaaccg tcaaccgaaa gcttgctgcc ggaatggaga gacgcttacg
     1921 agccgctgtc cctaccgatt gacggaatcg atgaagccgc tcttttcaag ctgatcgacc
     1981 agcgtcagat cgacaaactg ctcgggtttc ggcagcggct tcttgtccgg gtattgattc
     2041 tcgatcatga actggatggc tttcagcgac ggccgcggcg tcgtttgcaa cgtcggcgca
     2101 tagtcctgcc aggttccttc caacgcttcc tcgtcggtga tcttgccgta tttgcccagc
     2161 actttcttgc tgaattcttt gtcggtgcga tagcgctgca taccgcgcgt gaaagcgcgg
     2221 agaaagcgca gcgcggtgtc ccgctctttg gcgatgaagc tgcgggtggt aatcaccgta
     2281 cccgtgatgt cttctacccc cagcgcgcca aaattgatca gttcgcgcat ccccaacttg
     2341 gcggcggcag cgtagagcgg gaaggtgaaa gctcccgctt gcacggcacc gctttgcagc
     2401 gcggcgaaga cttccggctg ggcgccgaga ctgagcaggg cgacatcttt gttcggctcc
     2461 aggttggatt tcttgagcgc gatacgaatc acctggtcgg aagtggaacc gaacgttgta
     2521 atgcccgctc tctttccctt caggtccgag acatttttga tctcagggcg cgccatcaag
     2581 cgaaaagacg ataccgcgcc tgtagcgcct atgatcacga catcgccgcc cgccgtgttc
     2641 gcttgcaccg cggctatggc cccggcttga atgatcgacg cattgccgcc aatcatggcg
     2701 gcaaccatcc gtgagctacc gccgacccga atcagctcga cgtccaaacg ctcgtcgaga
     2761 aaatagcccg cttccttggc gatccacgtc gttccccacg atccagcgaa gctgggataa
     2821 aggatacgca tggaagtcgg cgccgccgct ccgaaaacgc tcgcgggagg ccatgcgagc
     2881 aggagagcaa acaaacatgc gatcttttgc gtgcgcaaca gcgtgtgaaa aattttcatc
     2941 aacgactcgg ggattagatc agcttatgtt tctttacttg atgaaacagc cgcatgcaat
     3001 tgtcgcggac gatattcaca acgtcggctc cgagtaccct gattgtactc gtgacgtaat
     3061 taagttcacc gatgcgggtg caagcactca tttcagaaga ggggggcggc caacactatg
     3121 ccgacggctg gagaacgact tatcgattcg cgatgatcgt tgatccgaat cgatcgcggt
     3181 cggaagctta gcgtttattg acgaggtaag aagcgggctt ggcttcaaag ccgcacatcg
     3241 cgatatcctc aatttcgttg gaagttatgc gctgcgggag cccggcggaa gagtacgcgc
     3301 tcaaatttgc cgccgaaact gaggctctaa ggtctcaaaa cacgttcttt ggaaagaaat
     3361 cgtttgatga agcaacaaca tagcgtggtc cgacccggcg cctcggcccg gatcattgcg
     3421 ctataacatt gcgccttcgc aataggtgcc ggtgattgtg cgtaatgaag aacgcaacat
     3481 actgaagccg atgcgctggg gtctcgtgcc gtcgtggacc gacgataagg ccatctggca
     3541 acggctgata aatgcccgtg cagagacact gcgggaaaga ccatcgtaca agcggctcgt
     3601 ttcaacgcag cgttgtctga ttccggcaga tggcttctat gagtgtaaac gtcaaaggaa
     3661 ccaggaagtg ccgatgtgga tctgccgacg gtgaattttt tcttagcgac actgatttcg
     3721 gatttcgcca aatttggctg cccaacaccg tagcttctgc ggggcgaact cctaatgctc
     3781 cgccgcttta atcgcgtccc gcagatagcg tagcgtcaga gtcgtaaagg cgatcatgtt
     3841 gggctctcgg tccggcaggc cggtctgctg cacgttgaca cgcgttacag ggccgcagac
     3901 gcagatcgtc gtgcggccgg gtggcaggcc ggatcggcct cgcgtgggac cggcgaggcc
     3961 ggattcggcg atgcaccagg tggcgcccag gcgctggcgc atggcgtcgg cgatggcggc
     4021 gatcatctcc ggcgtggtgc cgcggtagtt ctcaaacatc gatgccggca cacccgccag
     4081 cgccgtgcgc gagccaagcg tgtagagcac gctgccaccg gcgtagtact tggacgcgcc
     4141 ggccacccac agtagtgccg cggacacgag gcctccagtg gtagactccg ccacgcagac
     4201 cgtctcgcct cgctcggtga gtagggcggc gatctcgttc gcaaggtctt tctgttcgtc
     4261 agtaaggaag gacatgtacg cctccgtggg cttctagact tgtgctggcg tcacgttagc
     4321 acctcgatct gtgtcactcc atccctttcg gcgaaggctc aaagtcggat tactttcaag
     4381 cacgctggct tcgattgcgg attctttggt cgctgaaggt ccgagcgact tgatttgcaa
     4441 tcaagtgact ctacacagac gcgatttgct cgatgcgtca ttcaattcaa tccctgccgc
     4501 ctttgcgatg gtcttggcga aataggccgc cgatcgttag ggagataagc cgccgcgccg
     4561 gtcggctttt agcgaaaaac tctacgttga caagccaggt gagctgatgg atattgcacg
     4621 caatttgagc ttttattgtc cgagctgtcg ccgagataag ccgaccaaca attgtcgcta
     4681 cgtacggcgg ctcacttaat agttagagcg caaggtaaca acggagcgac aaatggaaaa
     4741 caaacgccac gcgccgcgga agttctttgc ggaaacacgt gagcattggc cgatcatgtc
     4801 cgtttacgag cgcttcgagc agattgttgc gatcgttctg agtctcgtga tctcagtggt
     4861 catcgcccta gcgctcgtgc agctggtcat ccgcatcgtg ccgctgttgc ttagcggcgc
     4921 gatagatcca ctggaacatg acgtctttca ggcgctcttt ggcatgatca tgacgctgct
     4981 catcgccatg gaattcaagc attcgattgt tcgcgttgct cttcggcgct cgagcatcat
     5041 ccaggtcaag accgtggtgc tcatcgcgct cattgcgttg agtcgcaagt tcgtcattct
     5101 cgatt
//
LOCUS       DASUTG010000190         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_178923, whole genome shotgun sequence.
ACCESSION   DASUTG010000190 DASUTG010000000
VERSION     DASUTG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178923"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VF089_04235"
     CDS             <1..263
                     /locus_tag="VF089_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173199.1"
                     /translation="PDCEVVLLGSIATGKYLDILNQAFSDQLRVPAEFVGLGDMSRGA
                     LLLRCVKEQRELNYITVAAASERLPRCDAAPEAVQPSVPDNL"
     gene            complement(283..1182)
                     /locus_tag="VF089_04240"
     CDS             complement(283..1182)
                     /locus_tag="VF089_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6173200.1"
                     /translation="MERNEMDVLSDSKKEILGNRTGIMTNPELSIELIEGAKQTVPSS
                     QGDATELASVRSEYIEEGVPIGSSPVMVEVSENGESVGQFEGLTVLLDKLGERLAFER
                     QGTRLYEAFLQKCESLTLEDGSGPSVEELRHICEEELEHFHLLQNAITALGGDATVQT
                     PSADVAGVLSHGVMQVVSDPRTTIPQTLQAMLTAELVDNDGWQMLQELAAELGQDDLE
                     GQCGRAYEEEQEHLEKVRDWLTSMTMDEVSGSTEALDVPEIDASEEEEDTQPKRSAKA
                     STRSGKSKSSSSSKSKSKKKKKR"
     gene            complement(1200..2399)
                     /locus_tag="VF089_04245"
     CDS             complement(1200..2399)
                     /locus_tag="VF089_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173201.1"
                     /translation="MSLDLMKEKGVGLDEQKFNWRDIAGIPTSKLDSDALTRVRIILM
                     NGIESEALRFQHACARMNKELQLALARVRRIEQHQQTMVNWLLPADLSPLETTIGFEQ
                     VAIEVTASVAEHEPDPYLAQVFRFGLLEDFDHMYRFSALMDRVTGCDSNNILQSYTDI
                     LPGRPTAVEHRAPEDDLREPYDRKKAALISKLNAATIMAGEHQTHDYYMTIGPMFADP
                     IARQLYAEIASIEEQHVTQYESIIDPEETWLEKWLMHETTEIYNYYSCLQYESHPKVK
                     AIWQRFLDYELGHFHYVLDLFKKFENRDPAEIIPESLPEPIEYRSHREFVRKTLNEEV
                     DLRARKTQFIDKDEEGADSPSVVYREQMNSKGSPSETVAAGYQWKPGTELAEETPDIK
                     KLQTKVA"
     gene            2697..3635
                     /locus_tag="VF089_04250"
     CDS             2697..3635
                     /locus_tag="VF089_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173202.1"
                     /translation="MIVSNAPVIEIHSVSKQFGDRMAVDNLDLEVPARTCFGLLGPNG
                     AGKTTTLRMIYGVTRPSSGTILVFGMDISQNSRAVRARLGVTLQQNVLIEALSPKDNL
                     RVFGRYHLLHEPELSRRAEELIDFLELRSHADVPVKRLSGGFQRRLAIALSLINGPEL
                     LILDEPTTGLDPAVRLALWSRIRELRASGTTVLVTTHYMDEAQRLCDQVAVVSAGKVI
                     GAGTPAELIAALAAETIELDCSIEEEATLLNGFAADAQCLRIGQRLMIYLEDTSGLID
                     HIRHSDHGDRRPIIVRPTNLEDVYLSLTGTSLEAGS"
     gene            3632..4399
                     /locus_tag="VF089_04255"
     CDS             3632..4399
                     /locus_tag="VF089_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6173203.1"
                     /translation="MNLSPAYALAVWLRNAAMYRRTWMWNLLPNFFEPVFYLFSIGIG
                     VGAYISQMEGVSYIAFIAPGLVCVAAMNGASYEVTYNIYVRLTFEKAYDAMLTTPIEP
                     DDILAGEVLWAITRACIYGGCFFVVLALFGLTPFPSSLWALLVIPLTGLLFAAIGITF
                     SLRIPNIDSFSFYFTLFLTPLFLFSDVFFPLEERLSPGWLWVAEALPLLHPVRLARAA
                     FSGNFSPVVLWDIGYSLVGSCLLLMWARRSVRQRLTN"
     gene            complement(4421..4732)
                     /locus_tag="VF089_04260"
     CDS             complement(4421..4732)
                     /locus_tag="VF089_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173204.1"
                     /translation="MERKVFKVDLLVRILLGAIAGWLTGKAADGERDSRRFSSGNKQL
                     RDVVCGVIGALVGQPLFFWVIIGSSSFGKYATAVLGAIAVVGAARLVGTRLHRARSTA
                     H"
BASE COUNT          953 a         1358 c         1329 g         1284 t
ORIGIN      
        1 gtcccgactg tgaagtggtt ctgctaggca gcatcgcaac cggaaagtat ctggacatcc
       61 tcaatcaagc cttctcagac cagctgaggg ttccggccga gtttgttggt ttaggtgata
      121 tgagccgcgg cgcgctcctg cttcgatgcg tgaaagaaca gcgggagtta aactatatca
      181 cggttgcagc tgcttccgag cgcctcccgc gttgcgatgc ggcgccggaa gcagtgcaac
      241 cgtccgttcc ggacaatcta tgactaagcc tctttgaact agctatcttt ttttcttctt
      301 cttgcttttg cttttagatg agctcgagct cttgctcttg cccgaccttg tcgatgcctt
      361 ggctgatcgc ttcggctggg tatcctcttc ctcttctgaa gcgtcgattt ctggaacatc
      421 gagcgcttcc gtcgatcctg aaacttcgtc cattgtcatg cttgtcagcc aatcgcgaac
      481 tttctcgagg tgctcttgtt cctcttcgta cgctctgccg cactgccctt cgagatcgtc
      541 ttgacccagc tctgcggcga gctcttggag catctgccag ccatcgttgt cgacgagctc
      601 tgccgtgagc atcgcctgca aagtctgcgg aatcgtcgtc cgtggatcac tcaccacctg
      661 catgacgccg tgactcaata ccccggcgac atccgccgac ggcgtctgca ccgtggcatc
      721 gccgcccaaa gcagtgatgg cgttttgcag caggtgaaaa tgctccagtt cttcctcgca
      781 aatgtggcgc aattcttcga ccgaaggacc cgagccgtcc tctagagtga gtgactcaca
      841 tttctgcaaa aaggcctcat agaggcgagt gccttgcctt tcgaacgcca gccgctcgcc
      901 taatttgtcg agcagaacgg ttagtccctc gaattgtccg acgctctcgc cgttttcgga
      961 aacttccacc atgaccggtg atgagccgat cggcacgccc tcctcgatat attcggacct
     1021 gaccgaagcc aactccgtgg catcgccttg tgagcttggc acggtctgtt tggcgccctc
     1081 gataagttcg atactcagtt ctggattggt cataattccg gtgcggttac cgagaatttc
     1141 ctttttcgaa tctgacagaa cgtccatttc atttctttcc ataagatcgt ctcctgtttt
     1201 tacgctactt tggtttgcag ctttttgatg tcgggagttt cttccgccaa ctcggtgcca
     1261 ggcttccact gatagccggc ggcaacggtt tcggacggcg agccttttga attcatctgc
     1321 tcgcggtaga ctaccgacgg cgaatcggcg ccctcttcat ctttgtcgat gaactgggtc
     1381 ttgcgcgcac gtaaatcaac ttcctcgttc agtgtcttgc gcacgaattc gcgatggctc
     1441 cgatattcga tgggctcggg cagtgattcg ggaataattt cggcggggtc gcggttttcg
     1501 aatttcttga acaggtcgag aacataatgg aagtgcccta actcgtaatc gagaaagcgt
     1561 tgccagatgg ccttcacctt tggatggctc tcgtactgca ggcagctgta gtaattgtaa
     1621 atctcggtgg tctcgtgcat cagccacttc tccagccagg tctcctccgg gtcgatgata
     1681 gattcgtatt gggtaacatg ttgttcttcg atcgaagcga tttcggcata caattgacgg
     1741 gcaatcggat cggcaaacat cgggcctatg gtcatgtaat agtcatgcgt ttgatgttcg
     1801 ccggccataa tcgttgccgc gttgagcttg ctgatcaacg ccgccttctt gcggtcgtaa
     1861 ggttcgcgta aatcgtcttc cggcgcccgg tgttctacgg cggtggggcg cccgggtaga
     1921 atgtcggtat aactctggag aatgttatta gaatcacaac ccgtaactcg gtccatcaag
     1981 gcggagaagc gatacatatg atcgaaatcc tcgagcagcc cgaagcgaaa gacctgcgcc
     2041 aggtaagggt cgggttcgtg ttccgctacg ctcgcggtca cttcgatggc gacctgctca
     2101 aagccgatgg tggtttccag cggcgaaaga tcggccggta acagccaatt gaccatcgtt
     2161 tgttgatgtt gttcgatgcg gcgcacccgg gccaacgcaa gctgcaattc tttattcatg
     2221 cgggcgcagg cgtgttggaa gcgcaacgcc tcggactcga tgccgttcat gagaataatt
     2281 cgcacccggg tcaaggcatc gctgtctagc ttgctggtcg gtatccctgc gatatcgcgc
     2341 caattgaatt tttgttcatc caagccaact cctttttcct tcatcaagtc taaactcata
     2401 ccgctctcct ttcggctgga acagttacag tggttagtga ccgcttagcg cggtgttaac
     2461 agtacgttaa gttgtgcaga aatttgcgat gagagactgc cggaggaaaa gttgtgcaag
     2521 cgaggtgcca cgacaaaaac gttgtgcagc aatgccttcg tctccgcctt ggaacatttt
     2581 gcttacaggt gttccgtagt tgataattgt tctgcgccgt cctcgagagt ggccacttgt
     2641 ggttggagaa aagataagtc agattttaaa gatattctca ccagccatgg ctctgtatga
     2701 tcgtttcaaa cgcgccagtt atagaaattc actcggtgtc aaaacagttc ggcgatcgca
     2761 tggccgtcga caatcttgac ctggaggtac cggcgcggac ttgtttcggc ttgctcgggc
     2821 ccaatggcgc cgggaaaacc accacattgc ggatgatcta cggcgtcacc cggccttcta
     2881 gcggtactat tttggttttc ggcatggata tcagccagaa ttcccgagcg gtgcgggccc
     2941 ggcttggggt tacgttgcaa caaaacgtct tgatcgaggc attgtcgccg aaagacaatc
     3001 ttcgggtatt cggccggtat catctgctgc atgagccgga gctgtcgcgc cgggccgaag
     3061 aactgattga ctttctcgag ctgcgttccc atgccgatgt cccggtcaaa cgcctgtccg
     3121 gcggctttca gcgccgcctt gcgatcgctt tgtctctgat caatggcccg gaactgctta
     3181 ttctcgatga accgacgacc ggcctcgatc cggcagttcg ccttgctttg tggtcccgga
     3241 tccgtgagct gcgcgcctcc ggaaccaccg tgctggttac cactcactat atggatgagg
     3301 cgcagcgttt gtgtgaccag gtagccgtgg tctccgctgg aaaagtcatt ggcgccggca
     3361 cgccggcaga actcattgcg gcgttggcgg cagaaacgat cgagctggat tgttcgattg
     3421 aagaagaagc gacgctgtta aatggctttg ccgccgacgc acagtgcctg cgaatcggcc
     3481 agcgtctgat gatttacctg gaagacacca gcggtttgat tgaccatata cgtcatagcg
     3541 atcacggcga tcgtcggccc atcatcgtcc gaccgactaa tctggaagac gtttatttgt
     3601 cgctcaccgg caccagtctc gaggctgggt catgaacctt tcacctgcat atgcattggc
     3661 cgtttggttg cgcaacgccg caatgtaccg gcgcacctgg atgtggaatc tgctgccgaa
     3721 tttcttcgag ccggtgttct acctattttc gattgggatc ggggtaggcg cttacatttc
     3781 acagatggag ggagtttcct atatcgcgtt catcgcgccg ggcctggttt gtgttgcggc
     3841 catgaatggc gccagctacg aagtcactta caatatctat gtacggctga cattcgaaaa
     3901 ggcttacgat gcgatgctga caaccccgat cgaacccgac gacattctcg ccggcgaagt
     3961 gttgtgggcg attacccgcg catgtattta cggcggttgc ttctttgttg ttctagcgct
     4021 gttcggtttg acgccgtttc cctcttcttt gtgggctttg ctggtgatcc cgcttaccgg
     4081 gctgctcttt gccgccattg gcataacctt ctcgctgcgc attcccaaca tagattcatt
     4141 cagcttttac tttactttat tcttgacgcc gcttttcttg ttctcggacg tatttttccc
     4201 attggaagag agattatcgc caggatggct gtgggtcgct gaggcgttgc cgctgcttca
     4261 ccctgtgcgg ttggcgcgtg ccgccttcag cggcaacttc agtccggtgg ttctgtggga
     4321 tattggctat tcgctggtgg gctcgtgctt gctgctgatg tgggcaagac gcagcgtgcg
     4381 ccaacgactg acaaattaga tatttacata tgggggaggt tcaatgagcc gtgctgcgag
     4441 cacggtgtag ccgcgttcca accaggcgcg ccgctcctac gacagcgatg gcgccgagca
     4501 ccgcggtagc atatttgccg aaggaactgc tgccgatgat gacccagaaa aacaacggtt
     4561 ggccaaccag agcgccaatc acaccgcaca caacatcgcg cagctgtttg ttgcctgagc
     4621 tgaagcgtct gctatcccgt tctccgtcag cggcctttcc ggtcaaccat ccggcaatcg
     4681 cacccagcaa gattcgaacg aggagatcca ctttaaagac cttcctttcc atcgtcgaag
     4741 cgctcggcgc ctttggcgtt ggccatacgc tcaaggtttc tatatcgcag gcccgcacca
     4801 aatcgggcga gcttcaaccc ggtcaaaccg ttctgccgag gtgaagggct gtgctaatct
     4861 gttcttccac cgcggtttta ctctcgccgg tgacttcgga gatctcgcac accaacagtt
     4921 ttcg
//
LOCUS       DASUTG010000191         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_771849, whole genome shotgun sequence.
ACCESSION   DASUTG010000191 DASUTG010000000
VERSION     DASUTG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771849"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2332)
                     /gene="mfd"
                     /locus_tag="VF089_04265"
     CDS             complement(<1..2332)
                     /gene="mfd"
                     /locus_tag="VF089_04265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6173205.1"
                     /translation="MLDSIKEGIPFPGIEFLLPYFYDELTSVFSYLPSDTLVCLAGAD
                     RVEAEVERFAKLTWDRNTKAKEEGRLVAPPGELFLSEHEWRAAIEPLAQVHCEALTIM
                     AASERLQESTLTVDSFLTNDIQHESLVKHGKEPSLAPLVERLGTWEGQKVIFVAPTRG
                     DAGRLRELLGHYEIDIPVVHQPVNALVNTAQLPRAIVLGHLNQGFRLPAAGLIAMTFD
                     EIFGTHKRHSTTVAKKHPSHFLTSLSELKQDDFVVHLDHGIGVYRGLKFLTVAGVQGE
                     FLHLEYEAGDRLYLPVDRINVVQKYIGGDGAQPSLDKLGGTSWERVKAKARKSIFAMA
                     EELIQLYAVREARQGNAFAPPDALYREFEAGFEFEETHDQQRAIDESLADMQRPKPMD
                     RLVCGDVGFGKTEVAMRAAFLAVEGGKQVAVLAPTTILAQQHLQTFRHRFRNHPVRIE
                     MVSRFLTKKEIEQVLKDSLRGSVDVVIGTHRLLQKDVEFKDLGLVIIDEEHRFGVTHK
                     ERLKKLRQMVDVMSLTATPIPRTLHMSIIGIRDLSIIETPPVDRLAIQTYLTRYDERV
                     IRDAILRELERGGQVFFLHNRVETIDRIAMKLADLVPEAKMAVAHGQMKPKDLERVML
                     EFLENETQVLVCSAIIESGLDFPNANTIIINRADRFGLAQLYQLRGRVGRSHRHAYAY
                     LLIPGEQAITPDAEKRLRALQEIDGLGGGFKLALHDLEIRGAGNLLGQQQSGNITAVG
                     FDLYTEMMENAVRELRVEEVTPDIEPEIRLGISAYFP"
BASE COUNT          536 a          663 c          618 g          546 t
ORIGIN      
        1 cagggaaata agcggatatg cccaaacgaa tttccggttc aatgtccggc gtcacttcct
       61 cgactctaag ttcacgcacg gcattttcca tcatttctgt ataaagatca aatccaacgg
      121 cggtgatatt accggactgc tgctgcccca aaagattgcc cgcgccgcgg atttccagat
      181 cgtgcagcgc cagcttgaat ccgccaccca acccgtcgat ctcttgaagc gcccgcagtc
      241 tcttctcggc atccggtgtg atcgcctgct cgccgggaat gaggaggtag gcataagcat
      301 gccggtgcga gcggccgacc cgccctctta attggtagag ttgcgctaaa ccaaatcgat
      361 cggcgcggtt gataatgatg gtgttggcat tgggaaaatc caagccagac tcgatgatgg
      421 ctgagcagac caatacctgg gtctcatttt ccagaaactc gagcatcact ctttccagat
      481 ctttgggttt catttgccca tgtgccacgg ccatcttggc ctcgggaaca aggtcggcca
      541 gtttcatggc gatccgatcg atggtttcaa ctcggttgtg caagaagaac acctgtccgc
      601 cacgctcgag ctcgcgcaag atcgcgtcgc gaattactcg ctcatcataa cgagtcaggt
      661 aggtttggat tgccagacga tcaaccggcg gagtttcgat gatgcttaga tcgcgtatac
      721 cgatgatcga catatggagc gtgcgcggga tcggcgtggc ggtaaggctc atgacgtcaa
      781 ccatctgacg aagcttttta agccgctcct tatgggtcac gccgaatcga tgctcctcat
      841 cgataattac caggcctaga tctttaaact ctacgtcttt ttgcagcaac cgatgcgtgc
      901 cgatgacgac atcgacactc cccctcaaag aatccttgag aacctgctcg atttcttttt
      961 ttgttaaaaa acggctgacc atttcgatgc gcacaggatg attgcgaaac cgatggcgaa
     1021 atgtttgtaa gtgctgttgc gccagaatcg tcgttggcgc cagcaccgcg acctgcttgc
     1081 cgccctccac tgcaagaaag gcggcgcgca tagcgacctc agtcttgcca aaaccgacat
     1141 cgccgcacac cagtcggtcc atcggctttg gtcgctgcat atcggcaagg gattcgtcga
     1201 tggcgcgttg ttgatcgtgc gtttcttcga attcaaaccc ggcttcgaac tcacggtaaa
     1261 gcgcatccgg cggcgcaaaa gcattgccct gtcgagcttc acggaccgca taaagctgaa
     1321 tcagttcctc cgccatcgcg aaaatggatt tgcgtgcttt ggctttgacc cgctcccacg
     1381 aggtgccgcc aagcttgtcg agtgaaggtt gcgcgccatc gccgccgata tatttctgaa
     1441 cgacattgat acggtctacc gggaggtaaa gccgatcccc agcctcatac tccaagtgca
     1501 agaattcgcc ttggacgccg gctactgtga gaaacttcag gccgcggtaa acgccgatgc
     1561 catggtcgag atgcactacg aaatcatcct gcttcagttc gctcaggctg gtgaggaaat
     1621 gacttgggtg ctttttggcc actgtagtgg aatggcgctt gtgcgtaccg aaaatctcat
     1681 cgaacgtcat ggcgatcaga ccagcagccg gcagacgaaa tccttgattg agatggccca
     1741 ggacgatcgc ccgggggagc tgagccgtgt tcaccagcgc attgactggt tgatgcacga
     1801 ccggaatatc gatttcgtag tggcctaata actctcggag ccgaccagca tcgccccggg
     1861 ttggcgcaac aaaaattacc ttttggcctt cccatgtacc aagtcgctcg acgagcggcg
     1921 ccaacgacgg ctctttgccg tgcttgacga ggctttcgtg ctggatgtcg ttggtgagaa
     1981 acgaatcaac tgtcaaagtc gattcctgca agcgctccga cgcagccatg atggtcaatg
     2041 cttcgcaatg cacttgagcc aaaggttcga tggccgcgcg ccattcatgc tcgctcagaa
     2101 agagctctcc cgggggcgcc actagacggc cttcttcttt cgctttggta ttgcgatccc
     2161 atgtcagctt ggcaaagcgc tcaacctctg cttcgacccg atccgctccg gctaagcaaa
     2221 ccaaagtatc ggatggcaag taagagaaca ccgaagtcag ctcgtcatag aaatatggga
     2281 gtaagaactc gatgccggga aaaggaatgc cttccttaat tgaatccagc accgtatttt
     2341 tctcgcgccg gtccatattc aaa
//
LOCUS       DASUTG010000192         3712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2106907, whole genome shotgun sequence.
ACCESSION   DASUTG010000192 DASUTG010000000
VERSION     DASUTG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2106907"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2883
                     /locus_tag="VF089_04270"
     CDS             <1..2883
                     /locus_tag="VF089_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6173206.1"
                     /translation="AAELRKRLEGEVKFDRYTLLLYSTDASIYQIEPIGVVMPRHKGD
                     VQAAIEVANRFELPILSRGGGTSLAGQAVCQAIVLDFSKYMRNVLEVNKEELWCRVQP
                     GLVQDELNAHVRSMGLQFGPDTSTSNRATIAGMIGNNSAGAHSLTYGKTLDHVLELTV
                     LLSDGSEVVLKDLSPDALDGKLGADGLEGQVYREVARLAEEHKNEILARYPKIMRRVS
                     GYNLDEFIKPQPFNLSRLITGSEGTLATIIEAKMRLVPKPKWTAMDVIHFQSDQEALE
                     CSQLVLQTAPYAMESTDKMILSLARGNIEQSRKLGFIQGQPESLLMVEYAGETQEEVK
                     AQVSKLEDLRAAQRIGYAATQAYKPEEVKAIWGVRKAGLGLLLGTKGDKKPIAFVEDT
                     AVAPAKLPEFIKRFKEIITSHDAIAGYYGHCSVGCMHIRPLINLKEPSELNKMVSISN
                     AISDLVLEFEGAMSGEHGDGLARSHFNKKLFGPALYQAFCEIKRAFDPNNRLNPGKIV
                     HAPSMTESLKIHPDYQTWMPRTTLDFSAQGGFARAIEMCSGMGECRKKLEGTMCPSYM
                     GTLDEEHSTRGRANALRTVLSGKVPKEDFTGTRLHEIMDLCLECKACKAECPSNVDMA
                     KLKYEFLDHYHRANGLPFRNRLFGGIARLNRVGAEFAPFANGIAGSKLNRWLMEKLVG
                     IDRRRPLPRFARQTFESWFGNHSPEGNGSRGEVVLFHDTFNDFNTPDVAIAATRLLEH
                     LDYRVRLINKRCCGRPMISKGMLGEAKNNAAWNIDQLAPFAERGIPIIGLEPSCLLTF
                     RDEYPEFLRTSAARKVAEQSFLLEEFLASKLAVGGFSVKSPGSRGKALLHGHCHQKAL
                     VGTAPTIAVLEVAGFDVSEIDSGCCGMAGSFGFEKEHYDLSVIIGNRRLAPAVKSAGT
                     DVEIAAPGISCRQQIEHLTGRRAKHPAELLWETFAPTSMDRSRQAD"
     gene            complement(2974..3408)
                     /locus_tag="VF089_04275"
     CDS             complement(2974..3408)
                     /locus_tag="VF089_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX6173207.1"
                     /translation="MIARELANAEQSDVCQLSGDPSQIEQAAAAAGLRVIKIDLKRLA
                     GKSGFLGRAARALNFPDWFGKNWDALNDCLTDLSWLDGRGWVIIFENSKDLAARKPQV
                     FHSAIEVFQSANDYWRQAGKPFWVLFHGPTDWVTGLERFDHE"
     gene            complement(3405..>3712)
                     /locus_tag="VF089_04280"
     CDS             complement(3405..>3712)
                     /locus_tag="VF089_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019059714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX6173208.1"
                     /translation="DHVRTQIKEIAIEELPGEARTTLQLIEKGGPFPYQRDGAVFGNF
                     ERRLPLKKRGYYREFTVPTPGRGDRGARRIVVGENGECYYTADHYRTFREIHKGLSR"
BASE COUNT          857 a         1000 c         1036 g          819 t
ORIGIN      
        1 gcggcggaat tgcgcaaacg gcttgaaggg gaggtcaagt tcgaccgtta tactctcctg
       61 ctttattcaa ccgatgcatc catttaccag attgagccca tcggcgttgt gatgccgcgc
      121 cacaaggggg acgtgcaggc agctatcgag gtggcgaatc ggtttgagtt accgatcctg
      181 tccagaggcg gcggcacttc gctcgccggg caggcagtgt gccaggcgat cgtgctggac
      241 ttctccaagt acatgcgaaa cgtcctggag gtaaacaagg aagagctgtg gtgccgggtg
      301 caacccggat tagtgcaaga cgagctgaac gctcatgtgc ggtcgatggg ccttcaattt
      361 ggtccggata cctcgacctc caatcgcgcc accatcgctg gaatgatcgg caacaactcg
      421 gccggcgccc attcactaac ttacggtaag accctcgatc acgtcttaga gctaaccgta
      481 cttttgtctg acggaagtga ggtggtgctc aaggatcttt ctccggatgc gcttgatggc
      541 aaactgggag cggatggact cgaaggacaa gtgtaccggg aggtagcccg cttagcggaa
      601 gaacacaaga acgaaatcct tgcacggtac ccaaagatca tgcgccgggt atcggggtat
      661 aatctagatg agtttataaa gccgcagccg tttaaccttt cgcgtctcat caccggctca
      721 gaaggaacac tggcgaccat tatcgaagcc aaaatgcgct tggtgcccaa gcccaagtgg
      781 acggcaatgg acgtcattca tttccagagc gatcaggagg cgctcgagtg ttctcagctg
      841 gtcctgcaaa cagcgcccta tgcgatggaa tccactgaca agatgatcct tagtctcgcc
      901 cgcggcaata tcgaacagtc gcgcaagctg ggatttatcc aaggccaacc tgaatccttg
      961 ctgatggtgg aatatgccgg ggaaacccag gaagaggtta aggcgcaggt gagcaaactt
     1021 gaagatctcc gcgcggcgca gcgtatcggc tatgccgcga cccaggctta caaaccagaa
     1081 gaggtcaagg cgatttgggg agttcgcaag gccgggctcg gtttactgct cggcaccaaa
     1141 ggcgataaaa agcccatcgc atttgtcgaa gataccgccg ttgcgccggc gaaactgcct
     1201 gagtttatca agcgtttcaa agaaatcatc accagccatg acgccattgc cggctactac
     1261 ggccattgtt ctgtaggttg catgcacata cggccgctga tcaatttaaa agagccatcc
     1321 gaactcaaca agatggtgtc catttccaac gcgatttcag acctggtcct tgaattcgaa
     1381 ggagcgatgt cgggtgagca cggcgatggc ttggcgcgca gccacttcaa taagaaactc
     1441 tttgggccgg ctctctacca agcattttgc gaaataaagc gcgctttcga tcctaataac
     1501 cggctcaatc ccggaaaaat tgttcatgcc ccgtccatga cggagtctct caagatccat
     1561 ccggattacc agacttggat gccgcggacc accctggact tcagcgcgca gggcggtttc
     1621 gcaagagcga tcgaaatgtg cagcggtatg ggcgagtgcc gaaaaaaact cgaaggcacc
     1681 atgtgtcctt cgtacatggg taccttggat gaggagcatt ctacccgtgg ccgagccaat
     1741 gctttgcgaa ccgtgctctc cggcaaagtg ccgaaggaag atttcaccgg cacgcggctt
     1801 catgagatca tggatctctg tctggaatgc aaagcgtgca aagccgaatg tccgtccaat
     1861 gtcgatatgg cgaagctcaa gtacgagttt ctcgaccatt accatcgcgc caatggattg
     1921 cccttccgca accggctgtt cggcggcatt gcgcgcttaa accgagtcgg cgcggaattc
     1981 gcgccgttcg ccaacggaat tgccggttca aaactcaacc gttggctcat ggagaagctt
     2041 gtcggcatcg atcgccgcag acccctgccg agattcgccc gccaaacttt tgaatcttgg
     2101 ttcggcaatc acagtccgga aggaaacggc tctagaggag aggtcgtgct gtttcatgat
     2161 actttcaatg attttaacac gcccgatgtt gccatagcgg cgacccgtct gctcgagcac
     2221 ctggattatc gtgtgcggct gatcaacaaa cgatgctgcg gtcggccgat gatctcaaaa
     2281 ggaatgttgg gcgaagcgaa aaacaacgcc gcatggaaca tcgatcagct tgcgccgttt
     2341 gccgaacgcg ggattccgat catcgggctc gaaccttcgt gcttgctgac gttccgtgac
     2401 gaatatccgg aatttttgcg aactagcgcc gcaaggaaag tggccgagca gagctttctt
     2461 ttggaggagt ttctcgccag caagctagcg gtgggcggat tttcagttaa atctccggga
     2521 tcccgcggca aagcattgct tcacggtcac tgccaccaaa aagctttggt cgggacggca
     2581 cccactatcg ccgtattgga agtcgcgggt ttcgatgtga gtgaaatcga ttcaggctgc
     2641 tgcggcatgg cgggttcctt tggctttgaa aaagaacact atgatctttc ggtaatcatc
     2701 ggcaaccggc gacttgcgcc ggcggtgaag tccgcgggga cggatgtgga gatcgctgcc
     2761 cccggtatct cttgccggca gcaaatcgag catttgacag gtcgtagagc aaagcacccg
     2821 gccgagttgt tgtgggagac cttcgcacca acttctatgg accgttcaag acaagctgat
     2881 taaacggcac agtcaaaagc gttcatgctt ctccgccatc gcagcggttg gcgcgctgaa
     2941 tccaacagcc gaactctcca acagcgcgta agatcattca tggtcgaatc tttctaggcc
     3001 ggtgacccaa tcagtcgggc catggaataa gacccaaaaa ggcttgccgg cttggcgcca
     3061 ataatcgttg gcagattgga atacctctat ggcgctgtga aagacctgcg gtttccgcgc
     3121 cgccagatct ttcgagtttt caaagatgat tacccaacct cggccgtcca gccaggaaag
     3181 atcggtcaag caatcattga gagcatccca gttcttgccg aaccaatcag gaaagttgag
     3241 cgcccgtgct gcgcgcccga gaaaacctga tttgccggca aggcgcttca aatcgatttt
     3301 aatgaccctg agtccagcag ctgctgccgc ttgctcgatt tggcttggat cgccgcttag
     3361 ctggcagaca tcggattgtt cggcattagc aagctctctg gcgatcatct tgataagcct
     3421 ttgtggattt cgcggaatgt ccggtaatgg tccgcagtat aatagcattc gccattttcg
     3481 ccgacgacaa tgcgcctggc gccgcgatca ccacgcccgg gtgtgggtac ggtaaattct
     3541 cgatagtaac cgcgcttttt gagcggcaag cggcgctcga aattgccaaa caccgcgccg
     3601 tcgcgttggt agggaaaggg cccgcctttt tcgatcagct gcagagtcgt tcgcgcttca
     3661 ccgggtaatt cctcgatggc gatctctttg atctgcgtcc ttacgtgatc tt
//
LOCUS       DASUTG010000193         6058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_921037, whole genome shotgun sequence.
ACCESSION   DASUTG010000193 DASUTG010000000
VERSION     DASUTG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921037"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VF089_04285"
     CDS             complement(<1..538)
                     /locus_tag="VF089_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX6173209.1"
                     /translation="MNKSRMPFKLVSWLIDRLAGYLWPVFDSTLGTRLRVYSALKGLA
                     ALAALGLIMLCLYAVVLIPFTPSIADLQKAKIDQPSMLISSDGKRIATYKPMNREWVR
                     LNRVSPHVINALIATEDHRFYQHYGVDVRRTVSGVFRTVLGDTEGGSTLTQQLARNLY
                     PDEIGRERTVTRKIKETIT"
     gene            778..1497
                     /locus_tag="VF089_04290"
     CDS             778..1497
                     /locus_tag="VF089_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010887116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6173210.1"
                     /translation="MAGKEMKFELDKLTKDSWRMFRIMGEFALGFDRMNQIESPAVTV
                     FGSARTQITSRYYDQARKLGRALVEAGYAVVTGGGPGTMEAANRGACEAKGTSVGLNI
                     LLEHEQKPNPYQTISLQFEYFYVRKVMLAKYSVGFVVFPGGFGTLDEMAEILTLVQTQ
                     KIHPFPIYLVGSSYWSGLVQWFRNTLAKEEAIAKSDLDLFKVLDDVTEIPSDIRRYHT
                     SARSAGFKVPREKDRKLAVGR"
     gene            complement(1504..2670)
                     /locus_tag="VF089_04295"
     CDS             complement(1504..2670)
                     /locus_tag="VF089_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173211.1"
                     /translation="MAKSKSQEKQIRSGLEALRHAGVAVDDPSPSLVQSLQEHFGKDH
                     QVDLAVVFTLGRIAEAAAVEALAEIENETHDKVLKKEIRRSLFKLSQKGLAVPEARTS
                     GVTSVTSLLRSTSDIEAYMSAVDGAGGRLLWIVKPQAGHGLQTIQAMVSDREGLLRLG
                     GARIRRKELRNMTREIKDKHGIEMISLPWEYADQTLYAAYEKSKAQGRSGFENFHEVR
                     AGIAAGKPTQQEHPIYRRLNPDDVTGGSWREQSRLLLEEPELRFWFVDEDWVTPYLSQ
                     LEEAKSSRLVLNQVQKEERLAGIVRDAVKEFCAGEIGPVLQRRLEDMALYFLQTGRDD
                     AARLSLAVAIQIATGDPGPLDISFLTGLVQKSFGYYLSQQKVKQADEPSFIVKP"
     gene            complement(2685..3629)
                     /locus_tag="VF089_04300"
     CDS             complement(2685..3629)
                     /locus_tag="VF089_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6173212.1"
                     /translation="MKLRPNRSPIKWPDGARIAITPCVAFETWPEDLGGPKTLQQENR
                     RSFPASAVTKKNLAIITDREFGERVGIYRMLDVFEKEKIQTSFFPTGITVQNFPEIFQ
                     EIAAQGHEVGTETWIHDYSYMKKRDKEKNDLRKTVDAVSKVVGKPPVGYLSTGVAPSA
                     ATPEIIVELGYSYWMDPQHEETPYTLKVGRKELTVLSYFQDLNDYSSYQRAGRTPRDL
                     LQMWKDCFDCLYEEGASDPKFMMWGNHPFVGGRPFRALLLREFIRYAKSHEKVWFATA
                     GQIAQWYRENYRDAQVETWPNFAFAGRQGKVSDLMSAR"
     gene            3857..4648
                     /locus_tag="VF089_04305"
     CDS             3857..4648
                     /locus_tag="VF089_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6173213.1"
                     /translation="MAEGKMIEYRVDNGVAVMEINRPPANAYTTELLKELDAAILDAR
                     FDDNVHAIVITGKLERFFSAGADIKMLGSASLSVKNNFALHGHEVLMRLENTPKVVIA
                     AINGHAQGGGLEIAMACDIRIAKKEGGRIGLPEINLGVIPGMGGTQRLPRLVGKARAM
                     ELCATGKLISFDEGLEMGLIHYIYEKEHFMEEVMDYARQFIVPNKSSLAVGKAKRAIT
                     TGSELSLPDGLAFERELLAQTFASEDGNEGVKAFFEKRTAQFKGK"
     gene            4979..>6058
                     /locus_tag="VF089_04310"
     CDS             4979..>6058
                     /locus_tag="VF089_04310"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6173214.1"
                     /translation="MAKMFIGGEPVDSVTKETYEIRNPANGAVVDSIPKGNEKDVQQA
                     IQAAETAFKEWADVTPEDRGSALMNACELIKKNSSEISQLLTQEQGKTLFEAGLEIHH
                     LVHGLEFYAGLASKVRGSHVPLPQKGAYGMVIRQPIGVCAGIVPWNFPLTLMGTKLGP
                     ALAAGNSIIIKPASTTPLATARVIELIAQATFGEGKAQKTLPKGTVNVVTGPGSSVGE
                     EILRNPRIRRIAFTGSTPVGRHVMEVAGREIKRVTLELGGSDPMIVMDDGNIDLAIKM
                     ADVGRYFNCGQMCLGVKRLFLQESIADKFLGGLVEILKKKTVGNGMSKETRMGPIHLE
                     SQRKEVEEQVEDAKSRGAKTLFGGER"
BASE COUNT         1456 a         1571 c         1648 g         1383 t
ORIGIN      
        1 cggtgatggt ttctttgatc tttctcgtga cggtccgctc acggccgatc tcatccggat
       61 ataggttgcg tgcgagttgc tgggtaagtg tagagccgcc ttctgtatcg ccgagcacgg
      121 tgcggaaaac acctgataca gtacggcgca cgtctacccc gtagtgttga taaaagcggt
      181 gatcttctgt ggcaatgagc gcgttgatga catgcggcga aacgcggttc aaccggaccc
      241 actcgcggtt catgggtttg tacgttgcga tgcgcttgcc atcagatgaa atcagcatgc
      301 ttggctggtc gatcttggct ttttgtagat cggcgatact tggtgtgaac ggtattaaaa
      361 ctacagcgta caaacagagc attatcagtc cgagcgcagc caacgcggcc agtcctttga
      421 gcgcggaata aaccctaagg cgcgtcccca gcgtagaatc gaatactggc caaagatagc
      481 ctgctagccg gtcgatcaac caactaacga gcttgaaagg cattctgctt ttgttcatct
      541 attcctgatt gggtggctga atattgcaac tgacgctaca ttaacacgaa ccaccagacg
      601 aatactttaa cagttcaatc agctaggttc agcccgcgca tgcaccgcca gaaatagaaa
      661 cgtctcatcc gtaaacgaaa gtggtgtatc atagttgagc tacgctgtag agcgcagcta
      721 atttaatcag gtctatcgtg aagcgcggca agccgattcg atgcggaggt aaaaaggatg
      781 gcagggaaag agatgaagtt tgagctggat aaactaacca aggactcgtg gaggatgttc
      841 cggatcatgg gtgaatttgc gcttggattc gaccgtatga accagattga atcccctgcc
      901 gtcaccgtct tcggcagcgc gcgaacgcaa attaccagcc gctattacga tcaggcgcgc
      961 aagttggggc gtgcactggt cgaagccggg tacgcggtgg tcaccggcgg cggtccggga
     1021 actatggaag cagccaatcg cggcgcctgc gaagccaagg gtacaagcgt aggtttgaat
     1081 atcctgctgg agcatgaaca aaagcccaat ccgtaccaga caatctcact gcaattcgaa
     1141 tacttctacg tgcgcaaggt gatgctggcc aaatacagtg tgggttttgt ggtttttccc
     1201 ggcggcttcg gcacattgga tgagatggcg gagattttga cgctggtgca aacgcaaaaa
     1261 atccatccgt ttccgatcta cttggtcgga tccagttatt ggtccggctt agttcaatgg
     1321 tttcgaaata ctctagccaa ggaggaagcc atcgctaaaa gcgatctgga tctgttcaaa
     1381 gtgctcgatg acgtcacgga aatccccagc gacatccggc gctaccatac gtcggcgcgg
     1441 agtgcggggt tcaaggtacc gcgtgaaaaa gaccgaaaat tggccgtcgg ccggtaatga
     1501 gcgctagggt ttgacgataa acgacggctc gtcggcctgt ttaaccttct gttgcgataa
     1561 gtagtatccg aaactcttct gcaccaagcc ggtgagaaat gaaatatcca gcggccccgg
     1621 gtcacccgtg gcgatctgaa tagccaccgc cagtgaaagc cgcgccgcat cgtcgcgacc
     1681 cgtttgaaga aaataaagcg ccatatcttc caaacggcgt tgcagcaccg ggccgatttc
     1741 accggcgcaa aactccttca ccgcatcgcg cacgatgccg gcaagccgtt cttccttctg
     1801 cacctgattg agcaccaaac ggctgctctt ggcttcctcg agttgagaca gataaggcgt
     1861 cacccaatcc tcgtccacaa accaaaagcg caattccggc tcttccagca aaagccgaga
     1921 ctgttcgcgc cagctgccgc cggttacgtc atcgggattg agccgccgat aaattggatg
     1981 ttcttgctgt gtgggcttgc cagcggcgat gccggcgcgc acctcgtgga agttctcaaa
     2041 gccgctccgt ccttgggctt tgcttttttc ataggcggca taaagcgtct gatcggcata
     2101 ttcccacggc agtgaaatca tctcgatacc atgcttgtct ttgatctcgc gcgtcatatt
     2161 gcgcagttct ttgcggcgga tgcgggcgcc gccgaggcgc agcagtccct cacggtcact
     2221 caccatagct tggattgtct gcaatccgtg acctgcctgc ggcttgacga tccagagaag
     2281 ccggcccccc gcgccatcaa cggcggacat gtaggcctca atgtcggagg tcgatctcaa
     2341 caaggaagtt acgctcgtaa cgccggaagt tcttgcttcc ggcacggcta atcccttctg
     2401 acttaactta aaaagcgaac ggcgaatttc cttcttgagc actttgtcgt gcgtttcatt
     2461 ttcgatctcc gccaacgcct cgacagcggc ggcttcggca attcgcccca aggtgaaaac
     2521 aactgccaga tctacttggt gatctttgcc gaaatgctcc tgtaaagatt gcacaagaga
     2581 cggagaagga tcgtcaactg cgacacccgc gtggcgcagc gcttcgagcc cgctgcgaat
     2641 ctgtttttcc tgtgacttgc tttttgccaa tgctgattcc gcggctatct cgcgctcatg
     2701 agatccgaaa ccttgccttg ccgtccggcg aaagcgaagt tcggccaggt ctcgacctgc
     2761 gcatcccgat agttttcccg ataccattgc gcgatctgac cggcggtggc aaaccatact
     2821 ttttcatggc tcttggcata gcggataaat tctctgagca atagggcccg gaaaggccgg
     2881 ccgccgacaa acgggtgatt gccccacatc atgaacttgg gatcgctggc tccctcttca
     2941 taaagacagt caaaacaatc cttccacatc tgcaagagat ctctaggcgt tctgccggcg
     3001 cgctggtagc tcgagtaatc attgagatcc tggaagtatg aaagcacggt caattctttt
     3061 cgtcccactt tgagcgtata gggcgtctcc tcatgttgcg ggtccatcca gtaactatag
     3121 ccaagctcga caatgatttc cggagttgcg gcactcggcg ccacaccagt ggaaagatag
     3181 ccgaccggcg gctttcctac caccttgctc acggcatcga cagtcttcct taaatcattt
     3241 ttctctttgt cgcgcttttt catgtaacta tagtcatgaa tccaggtttc ggtaccgacc
     3301 tcatgtccct gagcggcgat ctcctggaag atctcgggaa aattctggac tgtaataccg
     3361 gtcggaaaaa agctcgtttg aattttctcc ttttcgaata catcgagcat ccgatagata
     3421 ccgacccgct cgccgaattc ccgatcggtg ataatcgcca gatttttttt tgtgacagca
     3481 ctggctggaa aactacggcg attttcctgt tgcaaagtct tcggcccgcc cagatcctcg
     3541 ggccaggttt caaaggccac gcacggcgta atggcaatgc gcgcgccgtc gggccattta
     3601 atcggtgatc tgttcggtct aagcttcatg gttgcctcct cgcagtacaa aaattttcgt
     3661 tcagtcatgc cacagtttgc tacgcttgcc aacacgggct agcggctttg tttacggatc
     3721 acttagttgt tgagcatttt ttttcaaata agtgaaatca cacgatttct gcccttcttc
     3781 cgcaggatcc ggccttgact tgatcggtca tgccggttaa gtgagtgaga aatgaaatag
     3841 atgaggaggc ttagctatgg cagaaggaaa gatgatcgaa tatcgcgttg acaacggcgt
     3901 tgccgtaatg gaaattaatc gcccgccggc caatgcttat accaccgaac tgttgaagga
     3961 attagatgcc gcgattctcg atgcccgttt cgatgataat gttcatgcga tcgtcatcac
     4021 cggtaagctc gagcgatttt tttccgccgg cgccgacatc aaaatgttgg gcagcgcgag
     4081 cttatcggtg aaaaataact tcgcgcttca cggtcatgaa gttctcatgc gtttggagaa
     4141 cacgccgaaa gtggtgatag cggcgatcaa tggccacgct cagggaggtg gtcttgaaat
     4201 cgccatggcc tgcgatatcc ggattgccaa aaaagaaggt ggccgaatcg gcctgccgga
     4261 gattaatctc ggcgtgatcc cggggatggg tggcacgcaa cgcttgccgc gcctggtcgg
     4321 caaagctcga gccatggaac tctgtgcgac cggaaagctc atctcatttg acgagggact
     4381 cgagatgggg ctgattcatt acatctatga gaaagagcac ttcatggaag aggtcatgga
     4441 ttatgcgcgc cagttcattg tgcccaacaa gagcagtctc gccgtcggca aggccaaaag
     4501 agcgatcacg acgggatccg agctctcgct gccggacgga ttggctttcg agcgcgaact
     4561 cttggcccaa accttcgcca gtgaagatgg caatgaaggc gtgaaagctt tttttgaaaa
     4621 gcgaaccgcg caattcaagg gtaagtaaat cgtattcaaa aacaatcatt tttagatctc
     4681 ttaacgaaca actcgatgca agggatgcga tggatcgacg agccgagcgg actccgtgtt
     4741 acgttgaggc gaggcgattg agtgcaacca actaggtgag tctttttaaa cgatccgcat
     4801 tgaaggtaaa gtaactctat caaacaaagc aattaccaga tcgcttaaca aatgactcag
     4861 atgcaaggcg cgcgattgat cgacgagccg aggcgtactc cctgtacgtt gaggcgaggc
     4921 gattgagcgc aacgaagcag atgagcattt ttatgcgatc tgcactggag gagagctaat
     4981 ggcaaagatg tttatcggcg gagagccggt cgactccgtg actaaggaga cctatgaaat
     5041 ccgtaacccc gcgaatggcg cggtggtgga ctcaatcccg aagggaaatg agaaagacgt
     5101 tcagcaggcg attcaggccg cggaaactgc tttcaaagaa tgggccgatg taacgccgga
     5161 agatcggggc agcgcgctca tgaatgcctg tgagttgatc aagaaaaaca gcagcgaaat
     5221 ctcgcaactg ctcacacaag agcaaggcaa aactttattc gaagcaggtt tggagattca
     5281 ccatttggtg cacggcctag agttctatgc cgggctcgcc tccaaagtgc gcggatcgca
     5341 tgtgccgcta ccgcaaaaag gcgcttacgg catggtgatt cggcaaccta tcggtgtgtg
     5401 cgctggcatc gttccatgga acttcccgct gacattaatg ggcaccaagc tcggtcccgc
     5461 gctcgccgcc ggcaatagca tcatcatcaa accagcaagc acgacgccgc tggcaaccgc
     5521 tcgggtcatc gaacttatag cccaagcgac ctttggcgaa ggtaaagcgc aaaaaacact
     5581 gccgaaggga actgttaacg tcgtcaccgg gcccgggagc agcgttggtg aagagatttt
     5641 gcgtaacccg cgcattcgca gaattgcatt caccggttcg accccagtgg gccgtcacgt
     5701 tatggaagtg gcgggccgcg agatcaagcg cgtgactctg gaactcggcg gcagcgatcc
     5761 aatgatcgtc atggatgatg gcaacatcga cttggccatc aagatggcgg atgttgggcg
     5821 atactttaat tgtggccaga tgtgtctcgg tgtaaagagg ctttttctgc aagagtccat
     5881 cgccgataaa ttccttggcg gtctagttga aatcttgaag aagaagaccg tgggcaatgg
     5941 catgagcaag gaaacccgta tgggcccgat tcacctcgaa tcgcagcgaa aggaagtcga
     6001 agagcaagta gaagacgcta aatcgcgcgg cgctaagact ttgttcggcg gggagcga
//
LOCUS       DASUTG010000194         5158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_31600, whole genome shotgun sequence.
ACCESSION   DASUTG010000194 DASUTG010000000
VERSION     DASUTG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31600"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF089_04315"
     CDS             complement(<1..279)
                     /locus_tag="VF089_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6173215.1"
                     /translation="MFYLIQLLNGIQYGLLLFLLASGLTLIFGIMGVINLAHGSFYMI
                     GAYLAYWLSKTTGSLWLAIPLSIIIAFSIGLLLETTIFSRLYKRDHLYQ"
     gene            486..995
                     /locus_tag="VF089_04320"
     CDS             486..995
                     /locus_tag="VF089_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008758449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX6173216.1"
                     /translation="MADYPLTAAAISSEIKRAGIHFVVALPDRVTSQHLLKGMLSDGD
                     FHVVQVCKEDEGVSICSGLHAAGKRSVLMMQYTGLLDSINSIRGIGVEARFPVCMVVG
                     LLGKEPDVAPTQSKKYGVKIIEPILDVMGIEHHLIERDADTKKIVPAIDKAFAESFPV
                     AVLIGREPR"
     gene            998..1564
                     /locus_tag="VF089_04325"
     CDS             998..1564
                     /locus_tag="VF089_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6173217.1"
                     /translation="MMKRDEMLKAFARQRKDEIVVAVYMAAQEITHISPNDLNYTFTG
                     AMGQGSSHALGLALGRPDRRVIVFDGDGSLLMNLGSLVTIANQAPKNLVHCLCENGTY
                     ETNGAVPIPRAGRIRFAELAKTAGYRSTYELSDVAEWERELPRILKEDGPIFVDLKVE
                     PGEAYPENFPRLYSVDHRERFRRALAGS"
     gene            1836..2711
                     /locus_tag="VF089_04330"
     CDS             1836..2711
                     /locus_tag="VF089_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6173218.1"
                     /translation="MTSDATAQLLALITSVLYASALVSARAGLRYSTPATVTLFSILM
                     QNLLLWSAVFASGGVRAVPVPGVLLFTVVGIFQLGVRLLAYTGVEKIGASRSSALQSI
                     SPLVSAIIAVTILGEQPSLLIVLGTVLVVAGIVLVSWKPEQQLATFRRWHLLLPLGAA
                     FLTGMNHPIRRYVLAMANEPLFFSALMGTVSLAGFLIYLAASPRSQRLVWDRKAFWPF
                     IGTGLFETFSILLIITAISMGRVVVVAPIAASYPIWALIEARIFLRQMEPINLKILLG
                     ILSVVAGNAAIHLGR"
     gene            2771..3397
                     /locus_tag="VF089_04335"
     CDS             2771..3397
                     /locus_tag="VF089_04335"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6173219.1"
                     /translation="MKLFDISLSLSPQTIHWVTGTPLQLIERKRMSRGDTNNSSSIHT
                     SVHAGTHVDAPFHFVPDGVTIEALPLDLFIGPARVCELEPGSHITAVDINQLAIQDEH
                     RVLFKTRNSQLLHKGIYDPSFAAFSVDGAKALVDAGVRLVGLDYLSAAHADEQVPVHR
                     AFLDYGVVLLEGVDLSQVPPGRYELICPPVKLAGSDGAPCRAILRELN"
     gene            3472..4329
                     /locus_tag="VF089_04340"
     CDS             3472..4329
                     /locus_tag="VF089_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEX6173220.1"
                     /translation="MTNAKPAVIVHGGAGPIRDDSLSERLDGCKAAALAAWDILVQNG
                     SALDATEAAVTVLENNPLFNAGTGSTLNQAGKIEMDAAIMDGDSLRIGAVAAVEGIKN
                     PIRLARRILEDGRHVILAGEGALMFAREIGFPQCSPESLIAEHERKRWAEKHGTVGCV
                     VLDANGKLAVATSTGGIFNKLPGRIGDSPLPGCGTYADDRGGVSCTGLGEAIIRVVLG
                     KTAIDFLSAGIDPKAAARKSLELLEAKTGAQAGLIIIDCQGRIGYARNTTHMPVCLIE
                     DNQSIAFDS"
     gene            complement(4452..4706)
                     /locus_tag="VF089_04345"
     CDS             complement(4452..4706)
                     /locus_tag="VF089_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173221.1"
                     /translation="MEVHIKLAPAPYERLRNSYPIDSPVRELIDNARRLDHAIEGVLF
                     AGYTIVCNEEQARIFLETARQCCPEVIPQIEQAIKLAQAG"
     gene            complement(4781..>5158)
                     /locus_tag="VF089_04350"
     CDS             complement(4781..>5158)
                     /locus_tag="VF089_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173222.1"
                     /translation="HKSGFDRHIVQTGAKKDSRLPEAPTLSELMDKRKTPALSRNVAR
                     VMLVSATLGRPLIGTPGIPADRIKVLREAYLKAFAEPEVIQEAKKSRLDLETLPGEDV
                     ETQIREVMNQPKEVIDRVKKLSE"
BASE COUNT         1130 a         1374 c         1437 g         1217 t
ORIGIN      
        1 ctgatagagg tggtcgcgct tgtacagccg gctgaagatc gttgtctcca gcagcagacc
       61 gatcgagaag gcgatgatga tactgagcgg aatcgctaac cagagactgc cggtcgtctt
      121 tgacagccaa taagccagat aggcgccgat catatagaag ctgccgtgag cgagattgat
      181 gacacccata atcccaaaaa ttagggtcaa cccgctcgcc agcaaaaaga gcagcagacc
      241 atactgaatg ccgtttagta attgtattag ataaaacatc accaagggtt caaacgttca
      301 acagttcaaa agctcaaagt cagatcgctt tcgcgcatca accctttagg ccgttcaagc
      361 ggtgcaaagg aataggagtg atctaaaaca gcttcaacgg ttgaacctat aatgtagtct
      421 tcagagtttc tgacatcgaa ctattgaacc ttgaacggtt gagcagattt cccccgggag
      481 gctccatggc cgattaccca ttgacggctg cggctatcag cagcgaaatc aaacgcgccg
      541 gtattcactt tgtcgtcgct ctgccggacc gtgtcaccag tcaacatctt ctcaaaggca
      601 tgctgagcga cggcgacttt catgtcgttc aggtatgcaa ggaggatgaa ggcgtctcaa
      661 tatgcagcgg ccttcacgct gcgggcaagc gttcggttct gatgatgcag tacacgggtt
      721 tactcgactc gatcaactcg atccggggca tcggcgtgga agctagattt ccggtgtgca
      781 tggttgtcgg attacttggc aaagaaccgg acgtggcgcc gactcaatcc aagaagtacg
      841 gagtgaagat catcgagccg atcctggacg tcatgggaat cgaacaccac ttgatcgaac
      901 gtgacgccga cacgaaaaag atcgtacccg ctattgataa agcgtttgcc gaatcgtttc
      961 ctgtagccgt tctgattgga agggagccgc gctagtcatg atgaaacgcg atgaaatgct
     1021 caaagccttc gcacgccaac gaaaagatga aatcgttgtg gcggtttaca tggcggcgca
     1081 agagataacg catatttcgc ccaatgatct taattataca ttcaccggcg cgatggggca
     1141 gggttcatcc catgctctgg gtttggcgct cgggcgcccg gaccggcggg tcatcgtttt
     1201 cgacggagac ggcagtttgc tgatgaatct tggcagcttg gtcaccatcg ccaatcaagc
     1261 gccaaagaat ctggttcatt gcctttgcga gaacggcacc tatgagacca acggcgccgt
     1321 gcctataccg cgggccggac gaattcggtt tgccgaactt gccaaaaccg caggataccg
     1381 aagcacgtac gaattgtccg atgttgctga atgggagcgc gagctgccgc ggatcttaaa
     1441 ggaagacggg ccgattttcg tcgatctgaa agtcgaaccc ggcgaagcct atcccgagaa
     1501 cttcccgcgg ctatacagcg ttgatcatcg tgagcgattt cgccgcgcgc tcgcaggtag
     1561 ctgaaaagag cgtggtgatg gcgccacatc ttgctcggat ttggttgctc tcagagctca
     1621 gccccttttt atggacacga tcacgagaat ccgtggaagc tttggtaatc ggagctgcga
     1681 gtaaaaagga atctacagag ctgctagaga acggttgatc ttaaatatct gtaaacgact
     1741 ccgtaagagt ttcgcgcccg agcagaggct cgacaaatac aaactgcctt aacgctcaac
     1801 cagcggtctt ctgttgtcac aaatctcacc tggttatgac ctccgacgcc accgcgcaac
     1861 tgctggcttt gattacttcc gtcctatacg cctccgcgct ggtatcggcg cgagctggac
     1921 tgcgctattc gaccccggcg acggtaaccc tgttctcgat tctcatgcag aacctgctgc
     1981 tttggtcggc agtctttgcc agcggcggcg tccgtgcggt gcccgtaccc ggagttcttt
     2041 tgtttactgt ggtaggaatt tttcagctgg gtgttcgact tcttgcgtac accggggtgg
     2101 aaaagatcgg tgcttcgcgc agcagcgcgc ttcagtcgat cagtccgttg gtcagcgcta
     2161 ttatagcggt gacgattctc ggcgaacaac cctctcttct gattgttttg ggaacggtgt
     2221 tggtggttgc cggcatcgtg ctggtttctt ggaagccgga acaacagctt gcgacatttc
     2281 gccgctggca cctattgctg ccgctcgggg cggcctttct gaccggcatg aaccatccga
     2341 ttcgccgtta cgttctcgcc atggccaatg agccgctgtt tttttccgcg ctcatgggca
     2401 ctgtttctct agctggattt ttgatctacc ttgcggcttc gccgcgttcg caaaggctgg
     2461 tctgggatcg caaagctttt tggccgttca tcggcaccgg tttattcgaa accttctcga
     2521 ttcttctcat catcaccgcc atcagtatgg gccgggttgt ggtggtggcc ccgatcgctg
     2581 ccagctaccc aatctgggcg ctcattgaag cgcgaatctt tctgcgtcag atggaaccaa
     2641 tcaatctgaa aatactcctg ggcatattga gcgtcgttgc cggcaatgcg gcgattcatc
     2701 tgggcagatg agtgatactt gtgagtccaa atcgtgtggc ataagatccc acacgacagg
     2761 aggtgacatc gtgaaactct tcgatatctc tcttagcctt tcgcctcaga cgattcattg
     2821 ggtgaccgga accccgctgc aactgatcga gcgtaagcgt atgagccgcg gtgacacgaa
     2881 taactcatcg tcgatccaca ccagcgtcca tgccggaacg cacgtggatg cgccttttca
     2941 ctttgttccg gatggggtca cgatcgaagc gctgccgctg gatctcttca tcgggcccgc
     3001 cagggtgtgc gagctcgaac cgggtagcca tataaccgcc gttgatatca accaactcgc
     3061 gatacaagat gagcaccgag tgttgttcaa aacccgcaac tcgcagctac tgcacaaagg
     3121 catctatgat ccgagttttg ccgcgttttc agttgacggc gcgaaggcgt tggtggatgc
     3181 aggcgttcgt ctcgtcggtc tcgactatct ttcagcagct catgccgatg aacaggtgcc
     3241 ggttcatcga gcattcctgg attacggcgt agtgctcctc gagggcgtcg atttatcgca
     3301 ggttccgccg ggccggtatg agctgatttg cccgccggtg aaacttgccg gttcggacgg
     3361 cgcgccctgc cgcgcgattc ttagagaact gaattaacaa gctccttggt tcaaaatcga
     3421 aagtcgatcg cgcgcgtata ttccgctaag actccagatt gttttgaagc tatgactaat
     3481 gccaaaccag ctgtgatcgt tcacggcggc gccggaccga tccgagacga ctcgctatcg
     3541 gagcgactcg acggatgcaa ggcagcggcc ctggccgcct gggatattct tgtgcaaaac
     3601 ggatcggcac tggacgcgac cgaagccgcc gtgacagttc tcgaaaataa tccgctcttc
     3661 aacgcgggta ctggttcgac gctcaatcaa gccggcaaaa tagagatgga tgcggcgatc
     3721 atggacggcg attcgctgcg tatcggcgcc gtggcagccg tagaaggaat taaaaatccc
     3781 atcagactgg cgcgtcgaat cttggaggat ggccggcacg tgattctggc gggcgagggc
     3841 gcgctgatgt ttgcgcggga aattggcttt ccgcaatgtt ctccggaatc gctgattgcc
     3901 gagcatgaga gaaagcgctg ggcagagaag cacggcaccg tcgggtgcgt ggtgttggac
     3961 gcgaacggaa aactcgccgt ggctacctcc accggcggca ttttcaataa gctaccgggt
     4021 cgcatcggcg attcgccgtt gcccggctgc ggaacctatg ctgacgatcg cggcggtgtt
     4081 tcctgtactg gattgggcga agcaatcatt cgcgttgttc tgggcaaaac tgccatcgac
     4141 ttcctttccg caggcatcga tccaaaagcg gcagcgcgca aatctctgga attattggag
     4201 gctaaaaccg gagcccaagc cggcctcatc atcatcgact gccaaggacg aatcggttac
     4261 gctcgcaata cgactcatat gcctgtctgc cttatcgaag acaaccagag catagcgttc
     4321 gacagctaag cacggcgagt cgggttgatg tgtactagtc tagtcgggtg aaagccggac
     4381 gctctggcgt tagtcacccc tgtttgggcg aaccaaaaca gctatttttg caacgcgagt
     4441 tctagtcgta attagcccgc ttgggcgagt ttgatcgctt gctcgatctg cgggatcact
     4501 tcggggcagc attgtcgcgc tgtttctaaa aaaatccgcg cttgttcctc gttgcaaacg
     4561 atcgtgtagc cggcaaacag cacaccctcg atggcatgat caagccgcct ggcattgtca
     4621 atgagttcac gaacgggaga gtcgataggg taggagttac gcaatctctc atacggcgcc
     4681 ggagccaact tgatatgtac ttccatggcg ctaccttatc gacgatcgta gtgagaatga
     4741 agctgattga ttctgccctg gtgcgataca gtgatatcag tcattcggaa agctttttca
     4801 ctcgatcaat gacttctttt ggttgattca tgacctcgcg aatctgtgtt tcaacgtcct
     4861 cgccgggtag agtttcgaga tccagccggc tctttttggc ttcctgaatg acctccggtt
     4921 cagcgaatgc cttgagatag gcctcgcgca gaactttgat ccggtccgca gggatgcccg
     4981 gcgtgccaat gagcggccgt cccaaagtgg cggataccag catcacgcga gcgacattac
     5041 ggctcagtgc aggagttttc cttttgtcca tcagctcgga cagtgtcggc gcttccggga
     5101 gccgggagtc cttcttggct ccggtttgta cgatgtgcct atcgaagcct gacttatg
//
LOCUS       DASUTG010000195         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1810846, whole genome shotgun sequence.
ACCESSION   DASUTG010000195 DASUTG010000000
VERSION     DASUTG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810846"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VF089_04355"
     CDS             complement(<1..836)
                     /locus_tag="VF089_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173223.1"
                     /translation="MKTSFFVTVMSFLFVALTSANAQNYPTKVVTMIVPFAAGGPTDT
                     VARLLGGPMTKTLKQQIIVENVGGAGGTIAANRVAKAKPDGYTILIHHIGMSTAPALY
                     RKLAYNPITDFEHIGLINEVPMTLVAKNDFPAKDLKELIAYVKANKNKVNYANAGVGA
                     ASHLCGMLFMTAIETEVTTIPYSGTAPAMNDLLGGQVDFMCDQTTNTTSQIKAGKIKV
                     YGVTTKKRVASLPNVPTMDEAGLKGFEVSIWHALYAPKGTPKPVIDTLTKALQAALKD
                     PTV"
     gene            1089..1961
                     /locus_tag="VF089_04360"
     CDS             1089..1961
                     /locus_tag="VF089_04360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6173224.1"
                     /translation="MAERIRELLREYQQGKLSRREFMRQAIIATGSLAAANSLIASLM
                     PSAAVAAQVPPTDPDILTHNVQYDGKAGPIFGYLVRPIKAGKYPAVIVVHENAGLNDH
                     IRDVARRFAKEGYVALAPDYLSRHGGTAKANPKGGGLSNIRELAPWQNVAEDTQAGIQ
                     YLRVLPDVRGDRIGMVGFCWGGEVTFATVTQVPAIRAAVVYYGRSPDPDRLKNIKTPI
                     MAHYGEKDPGVNKGIAETEVAMKKYNKTYDHKIYPGAPHAFNNDTNPERYNAAAAREA
                     WDRTQEFFKNHLKA"
     gene            2081..3040
                     /locus_tag="VF089_04365"
     CDS             2081..3040
                     /locus_tag="VF089_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173225.1"
                     /translation="MKLLSLVSLLWLCVIGEISAAPLRLSYSVVGPTVAGIWMAHDYG
                     IFEKYGLDVQLVYIPSSGTNIQALLGGSLDMSAPGISGVVLAAARGAPVLAIGAMTNR
                     PPMTLYVHPDITRTEQLKGQTLGITRFNSTAHTVTTLILRKLGLEQSATMRPLGGQPE
                     IQAAFEQKQIAGMVTSVKPRAPARALLNAADLDIPFAMNVMAVTREFLRKNPDTVERV
                     MKAYIEAVAKMNNDKQATLKVLAKYFKRNDPGFLDETYAIVTRFTEKIPRVDARNVAT
                     VLEFEPVKGVDAETLTPKVIDNSVVEKIAKEGFIEKVFGNSLR"
BASE COUNT          810 a          932 c          948 g          782 t
ORIGIN      
        1 accgttgggt cttttagcgc agcctggaga gctttcgtca aagtatcgat caccggtttg
       61 ggcgtgccct tgggcgcgta gagagcatgc cagatcgaca cctcaaaccc ctttaatccg
      121 gcctcatcca tggtcggcac attgggcagc gaagccacgc gctttttggt tgtcacacca
      181 tataccttga tctttcccgc tttgatctga ctcgtagtgt tggtggtctg gtcgcacata
      241 aagtccacct ggccgccaag aagatcgttc atcgccggcg ccgtgccgct gtacgggatc
      301 gtcgtcactt cggtctcgat ggcggtcata aacagcatac cgcatagatg agaggcggca
      361 cccacgccgg cattggcgta attgactttg ttcttgttag ctttaacgta cgcgattagc
      421 tcctttaggt ctttggcagg aaaatcattc ttcgccacca gcgtcatcgg cacttcgttg
      481 atcaggccaa tgtgctcgaa gtcagtaatc gggttgtagg ccagcttgcg gtatagggcc
      541 ggggcggtcg acatgccgat gtgatgtatc aaaatcgtgt aaccgtcggg ctttgcctta
      601 gctaccctgt tggcggcaat cgttcctccg gcgccaccga cgttttcgac gataatctgc
      661 tgcttcagcg tcttggtcat cggcccgccc agcaatctgg ccacagtgtc ggtcggtcca
      721 ccggccgcaa aagggacgat catggtgacg accttggtcg gataattttg cgcatttgcc
      781 gaagttagcg cgacaaaaag gaaactcatc acagttacga aaaaactcgt cttcatttta
      841 atcctccctt tccgcattca atcattaaac cgatatagct acttaatgat aacaaattat
      901 cgcgcctcgg gtagatacct gtcgaatcga ccagggggtc acacgtgctc tcatcgaacg
      961 gcatgaaaaa caggtcgtca catccgaaca acagcggacg gatgtcttga cctggctgtt
     1021 cggatcaata tataggttgc acgctgggcc gaatgaatgg ctagctaaac caacacaaag
     1081 gagagcttat ggccgagagg atcagagaac tgctgcgcga ataccagcaa ggtaaattgt
     1141 cgcggcggga atttatgcgc caagcgatta tcgccacagg gagcctggca gcggcaaaca
     1201 gcctgattgc cagccttatg ccttccgcgg cagtcgcagc tcaagtcccg cccaccgacc
     1261 cggacattct cacacacaac gtccaatacg acggtaaagc cggaccgatc ttcggttatc
     1321 tcgtccggcc gataaaagcc gggaaatacc cggcagtcat agtcgttcat gaaaacgccg
     1381 gtctcaacga tcacattcgc gatgtggctc gtcgattcgc gaaggagggt tatgtcgctt
     1441 tagcgcccga ttacctgtcg cgccatggcg gcacggccaa agccaatccg aagggcggcg
     1501 gcttgtcgaa tattcgtgag ctggcgccgt ggcaaaacgt cgcggaggat actcaagccg
     1561 gcattcaata cctgcgcgtc cttcccgatg tccgcggcga tcgcatcggc atggtagggt
     1621 tttgctgggg cggtgaagtg acgtttgcga cggttactca ggttccggct atcagggcag
     1681 cggtggttta ctacggccga agtccggatc cggatcgcct gaaaaacatc aagaccccga
     1741 tcatggcgca ctacggtgaa aaggatcccg gcgttaacaa aggcattgcg gagacggaag
     1801 tcgcgatgaa aaaatacaat aagacatacg atcataagat ttatccgggc gcgccgcacg
     1861 cctttaataa tgacactaat ccggagcgct acaacgcagc ggcggcccgt gaggcatggg
     1921 accgaactca agaattcttt aaaaaccatc tcaaggcttg agcctcgcct tgtgaattag
     1981 atgcgagcgg gtcctcgatg agcgagcccg ctcgctcgag gccgtacatt cgaaatattc
     2041 tcaatggtat tttcttactg ttaggatcaa tcgaagcgcc atgaagctgc tctccttggt
     2101 ttcgctgctc tggctatgtg tcatcggcga gatttcggcg gcgccgctgc ggttgtccta
     2161 ttccgtggtc ggtccgaccg tagcaggtat ttggatggcg catgactacg gaattttcga
     2221 aaaatacggt ttagacgttc agctcgttta tatcccgtcg agcggcacga atatccaggc
     2281 gcttctcggc ggcagccttg acatgagcgc tcccggaatc agcggcgttg tcctagcagc
     2341 agcccgcggc gcgcccgtgc tggcaatcgg cgccatgacc aatcgcccgc cgatgacctt
     2401 gtacgtgcat cctgacatca cccgaaccga acaattgaag ggtcagactc ttggcatcac
     2461 aaggttcaat tcgacagctc acaccgtgac gacattgatc ttgcgcaagt taggattgga
     2521 acagtccgcg accatgcgtc cgctgggcgg ccagccggag atccaggccg cgttcgaaca
     2581 aaagcagatt gccgggatgg tgacctcggt caagccaaga gcgccggcgc gcgctctgct
     2641 caatgcggcg gacctggata ttcccttcgc catgaacgtc atggccgtga cccgggaatt
     2701 tttacggaag aaccccgata ccgtggaacg ggtaatgaag gcttacattg aagccgtggc
     2761 caagatgaat aatgacaaac aggcaactct caaagtgctg gcgaaatatt tcaagcgcaa
     2821 tgatcccggc tttctcgacg agacctatgc tattgtcact cgcttcaccg agaagatacc
     2881 acgggtcgat gcgcgaaatg tcgctacggt cctggagttc gagccggtca agggcgtcga
     2941 tgcggaaacg ctgacaccca aagtcatcga caacagtgtc gttgagaaaa ttgccaaaga
     3001 gggatttatc gaaaaggttt tcggaaattc gctgcgctga aacccgaact cttaggatag
     3061 aaccattaga attcgacacg caacaaatca ggccgtgggc tcgttcggtc tggcttctga
     3121 acttctcaag attgtggttg ttctagatcg ggcgagcgta caaaagctac gtctgcgcga
     3181 tgtatttttt ctcaatagct ggtcttctga tccttgttgc agttggcctg ccggactaac
     3241 cgcggcgcaa cggttaccgt acaacgtcga gattgacgat actgtcgtgc gcgagataga
     3301 gaaaaaaggg ctatctagcg ctctgtcgtt gaacgagttg gttcagcaaa atcggcgcga
     3361 ggcttcaccg ccgcagaaga gcaggcgact tagaatacaa tggcacagaa attcacttcg
     3421 acactgattc tgttcggaac gttggtattt agcctggcat ccccgatttg gg
//
LOCUS       DASUTG010000196         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_625101, whole genome shotgun sequence.
ACCESSION   DASUTG010000196 DASUTG010000000
VERSION     DASUTG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625101"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF089_04370"
     CDS             <1..250
                     /locus_tag="VF089_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HEX6173226.1"
                     /translation="LPPDAEVEVLDVFGTVHALIANPADFGLTEVEDACITPNIPPFS
                     CKQPDEYLFWDGIHATKAIHEIFADVALEVLNPVVAKK"
     gene            complement(389..1018)
                     /locus_tag="VF089_04375"
     CDS             complement(389..1018)
                     /locus_tag="VF089_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173227.1"
                     /translation="MLKHAVQVYIGYLLLLILVAGCATTSVPTASEVSELLGGKKTLI
                     LLRLTVDIDGKPSEPFDASLADDNVGLAFITGDRVRRLEFYRSPSDQARQDGWIYLVE
                     DPGTYYLAIQPPRDRDAFSYQFQFRSAPRWRIDALAGSRIVYAGRLHINAVTEKGFLF
                     TRRTVYDLSKTAVRDERSLARELAAKSFPDLGSPIAVLMEPLGVPTAGP"
     gene            complement(1033..1368)
                     /locus_tag="VF089_04380"
     CDS             complement(1033..1368)
                     /locus_tag="VF089_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173228.1"
                     /translation="MVGLIGAAVGVASLAAAEAGVKDPRIAHYQRIELRGALLRQHES
                     THGFIFFHIPPDRQFPDAAELVVRLNDAVDESSLSVRLPLSGLILQTGSPGVGIEKTG
                     ERDGAKAGE"
     gene            1761..2279
                     /locus_tag="VF089_04385"
     CDS             1761..2279
                     /locus_tag="VF089_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173229.1"
                     /translation="MECITTSLRDAKPGVRFLYLDEFRQRVFYYGYPENENDRKKYFT
                     SLGNEPALKQRVIEMGIRYVISIYGATEQKSAPLGGFLGGAGGGFTWFGMAWDRKSRL
                     EASIFDLQQVDDLGTVRAFAEGHPWFICIGIGPFCAPIGAAAFTESTACKGLAEALAA
                     VLTGRHSPEPVK"
BASE COUNT          537 a          637 c          642 g          503 t
ORIGIN      
        1 gttgccgccg gatgccgaag ttgaagtgct tgatgttttc ggaacggttc acgcactgat
       61 cgcgaatccc gcagacttcg ggctgactga agtcgaggat gcctgtatta cacccaacat
      121 ccctccgttt agctgcaagc agcccgatga atacttgttc tgggacggca ttcatgcgac
      181 caaagccatc cacgagatct tcgcagacgt agcgctcgaa gtactaaacc cggttgtagc
      241 gaagaaataa agaacaaggg cgctgcgact tcaggcagtg gcttactgga ttgatcgcgc
      301 ggattgaagc agcgaagctg ctctccgatc tctcgacctt cgtgggctcg ctgtcagctg
      361 tgagggctcc tgatcgtccg aacaacgatc acggaccggc tgtggggacg ccgagcggct
      421 ccatcaagac ggcgatagga gaccccaggt cagggaaact cttggccgct aattctcgcg
      481 caaggctacg ctcgtctcgc accgccgtct tggacagatc gtagacggtg cgtcgggtga
      541 acaagaatcc tttctcggta acggcattga tgtgcagccg gccggcataa acgatgcgtg
      601 aaccggccaa tgcatcaatg cgccagcgcg gagccgagcg aaactggaat tgataactaa
      661 aagcatctct gtcacggggc ggctggatag ccagatagta agtaccggga tcttcgacga
      721 gatagatcca accatcttgc cgcgcttggt ctgagggcga gcggtaaaac tcaaggcgcc
      781 taacacggtc accggttata aaggcaagtc ccacattatc gtccgcaagg ctcgcgtcaa
      841 acggttccga aggttttcca tctatatcca ccgtgagccg caacagtatg agcgtcttct
      901 tgccacccaa tagctcgctg acctcactag ccgtcggcac gctggttgtc gcgcagcctg
      961 cgaccagaat gagcaggagt aaatagccga tataaacctg cacggcgtgt ttaagcatga
     1021 tccgctcgct ggttattcgc ccgctttcgc gccgtcgcgc tcacccgtct tctctattcc
     1081 cacgcccggc gatcctgttt gcaaaatcaa cccgctgagc ggcaagcgaa cgctcaagct
     1141 cgactcatct acggcgtcat tcaatcggac gaccaactcg gccgcatccg ggaactgcct
     1201 gtccggcggt atgtggaaaa aaatgaaacc atgtgttgac tcgtgttggc gcagcaatgc
     1261 tccacgcagt tcaattcgtt gatagtgggc gattcgtggg tccttgacac cagcttcggc
     1321 ggcggcaagg ctggcaactc ccaccgctgc tccaattaaa ccgaccatgg accccgcttg
     1381 acggttctgg ggaggcattg gaaatgcggg tgaagggtcg ctgacactcg aagcaaaata
     1441 ctttgacacc acgaacaaag aagttgccac aataaacgtc gagggtcgga ttggaagtgg
     1501 tttttttggt ggtgctctgt ctgaagccgt tgacaaagca gcggaaaaag ttgcggacta
     1561 caccaaggca aattttcatt aactgcgata tgcttcgtat aggttttccc ctgtttcatc
     1621 tcattctttc cgctattctg ttattggccg gatgtacaac tcctcaaatc gactcaacgc
     1681 agcgaatcgc tggcgcgctc gaaccccatg ctaccgtagc gatccttata tcgacagatt
     1741 cggatctgga acgcgaagcc gtggaatgca tcaccacatc gttgcgggat gctaagccag
     1801 gagtgcggtt tctctatctg gacgagtttc gtcaaagagt cttttattat ggctatcctg
     1861 aaaacgagaa tgaccgcaaa aagtatttca cttcgctcgg taacgagccg gcgctcaagc
     1921 aacgcgtgat cgagatgggt attcgttatg tgatttcaat atacggtgca acggagcaaa
     1981 aaagcgcgcc actcgggggt tttttgggag gagcaggcgg cggctttacg tggtttggca
     2041 tggcgtggga ccgcaagtct cggctggaag catcgatctt cgacttacaa caagtcgatg
     2101 acctagggac ggtgcgtgcc ttcgcagaag gccacccgtg gttcatctgc attggcatcg
     2161 gacctttttg cgctccgatc ggcgcggccg ccttcacaga atcgaccgcg tgcaaagggt
     2221 tggccgaggc actagcggcg gtactcaccg gacgacactc cccagagccg gtaaaatgac
     2281 ccgcggcgcc gagggagaaa ctcgcccaac tcacaatga
//
LOCUS       DASUTG010000197         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_328273, whole genome shotgun sequence.
ACCESSION   DASUTG010000197 DASUTG010000000
VERSION     DASUTG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328273"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..768)
                     /locus_tag="VF089_04390"
     CDS             complement(37..768)
                     /locus_tag="VF089_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6173230.1"
                     /translation="MSVQRRNRAERQLDQDGVREFYNDIYYRDRIKTNSPGFHLRRLA
                     RRFEPWSGRRALDIACGTGGWLQAIAGRGAITAGVDISQVALDGCRNAVPEAELHCGP
                     AERLPFADRQFDFISCLGALEHFLDIQASLQEIIRVAKADASILLLVPNSDFLPSRLG
                     LYNGTLQAAVKEQAFSLLEWQKLFQSAGLRVKARWKDLHILSAEWIFRGAYYEWPLRA
                     AQAIALPFWPLNWQYQVYYLCGLNK"
     gene            complement(770..1270)
                     /locus_tag="VF089_04395"
     CDS             complement(770..1270)
                     /locus_tag="VF089_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173231.1"
                     /translation="MTAKIFLTPWAWTCMALGACAVLGGGVLLRSSLWGLGLSPDSVV
                     YIGAARSLLTGHGFSLPGDPAPFSFSPITHYPPLYSSLLALVGTLADPLDGAIWLNVA
                     AFSTNIYVAGFLLFAVLGSWQLALLGSLFTLTAFPLVQAHTMAWSEALFIMLEFLSIL
                     LLLRAR"
     gene            complement(1421..2347)
                     /locus_tag="VF089_04400"
     CDS             complement(1421..2347)
                     /locus_tag="VF089_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6173232.1"
                     /translation="MGLFQQPARACAVSIIIPVFNEAESLRELLEDIRALEIAQSEIL
                     VIDDGSVDGSAEVAFNAGANVVRHPYNIGNGAAVKSGIRAAQGRRLVLMDGDGQHQPA
                     DIPKLLAQCEGYHMIVGERAKGSKLRFHRNAANLFYNLLASYVTRFKVKDLTSGFRVL
                     SRSDALRFIDLLPNTFSYPTTLTLAFLRSGLTIKYVPIQSLYRAGQSKISLLTDGIRF
                     LLIITKIATLFSPFRIFLPVSMFFFFAGLANYIHTYFTQYRFTNMSVFLLTTAVIIFM
                     LGLVSEQIALLRMEQRASFRSGRTEGRHEQEL"
BASE COUNT          645 a          689 c          651 g          509 t
ORIGIN      
        1 ttccaggtga taacgagaat gtccatgcaa gttgagttac ttgttcaaac cacagaggta
       61 gtagacttga tactgccaat tcagcggcca aaacggcaag gcgattgcct gagcagctct
      121 gaggggccat tcataatagg cgccgcgaaa tatccattcc gccgataaga tatggaggtc
      181 cttccaacgc gctttcaccc gcagcccggc ggattggaaa agcttctgcc attcgagcag
      241 tgaaaacgcc tgttctttca ccgctgcttg caacgtaccg ttgtacaatc ccaatcggct
      301 cggcagaaag tctgagttgg ggaccagcaa caaaatagaa gcatccgctt tggcgacccg
      361 gattatctct tgaagcgagg cctggatgtc gagaaagtgt tcgagcgcgc cgagacagga
      421 gatgaaatca aactgtcgat ccgcaaacgg cagccgttcg gccggcccgc agtgaagctc
      481 ggcttcgggc acagcgtttc tgcagccatc caatgcaact tgcgaaatat ctactcctgc
      541 ggttatagcg ccgcgtccag caattgcttg taaccaacct cctgttccgc atgctatgtc
      601 cagggcccgc cgaccgctcc aaggttcgaa acgccgcgcc agccggcgca aatggaaccc
      661 aggagagttt gttttgatcc ggtcccgata gtaaatatca ttgtaaaatt ccctgacgcc
      721 atcctggtcc agttgtcgct ctgctctatt acgtcgttgc acgctcatct caccgggctc
      781 gcagcaagag caatatactg agaaactcaa gcatgatgaa caatgcttcc gaccatgcca
      841 tcgtatgcgc ctgcaccagc ggaaatgcgg tgagggtgaa aagggaaccg agcaaggcta
      901 actgccaaga accaagcacc gcgaaaagca aaaatccggc gacgtaaatg ttggtactga
      961 acgcggctac attgagccat atagccccat cgagtggatc agctagagtg cccacgaggg
     1021 ccagcaatga agaatacagc ggcgggtaat gggtgatcgg tgaaaatgaa aaaggcgccg
     1081 ggtctcccgg caggctgaaa ccatggcctg tcaaaagact tctcgcagcg ccaatgtaaa
     1141 ccaccgagtc cgggctcagc cctaatcccc acaggctgct ccgcaagagc actccaccac
     1201 ctaggaccgc gcaggcgcca agcgccatgc aggtccatgc ccatggtgtg aggaatattt
     1261 tagccgtcat caaagccgca gtcaccgccg cttatagcct ctcccaagat tccacgacgc
     1321 ttgagaccga gcacaatcac gcaaagcaaa cggccggtgc agcaaatcat gcgtgtgtct
     1381 agtggttagc acgaactaag agcagcgaag ccggcaacct ctatagctct tgctcgtgcc
     1441 taccttctgt gcgccccgac ctgaaggatg ctctttgctc catccttagt agcgcgattt
     1501 gctccgacac cagtcccagc ataaaaatga tcacggcggt ggtcagcagg aacaccgaca
     1561 tattggtgaa acgatactgg gtgaagtacg tgtggatata attggctaga ccggcaaaga
     1621 aaaaaaacat gctgaccggc aggaagatac gaaacggcga aaaaagcgtg gcgatttttg
     1681 taatgatcaa caaaaaccgg atcccatcgg taagcaggct gatcttactc tgcccggcgc
     1741 gatagagcga ctggatcggc acatacttga tggttagccc ggaacgcaaa aatgccaagg
     1801 tcaaggtcgt cgggtaagaa aacgtattgg gcaagaggtc gataaaccgc aaagcatcgc
     1861 ttcgcgacag aacgcggaaa ccagaggtaa gatcttttac tttgaaacgg gtcacataag
     1921 aagccaacag attgtaaaag aggttggccg cattgcgatg aaatcgcagt tttgagcctt
     1981 tggcacgctc gccgacaatc atgtgatacc cttcgcactg cgccagcaat ttgggaatat
     2041 ccgccggttg gtgctggccg tcgccgtcca tcagcaccaa ccgtctcccc tgggcagctc
     2101 tgataccgct cttcaccgcg gccccattgc cgatattgta gggatgtcgc accacattgg
     2161 cgccggcgtt gaacgctacc tcggcgctac catcaacaga gccatcgtcg attacgagga
     2221 tttcagactg ggcgatttca agggcgcgga tatcttccaa aagctcacgg aggctctccg
     2281 cttcattgaa gacaggaatg atgattgata cggcgcacgc tctagcaggc tgctgaaaaa
     2341 gacccatatg cttcgttgcc gctcaatcgc ctcgctgcga cgtactgtaa gtacgccttc
     2401 gcgcgtcgat ttttcgcgcg tcttctatct tcatttcgtt gtatgaacat actcgaaaca
     2461 aataggacgg gacagatcga tcgcgcctgg gtcg
//
LOCUS       DASUTG010000198         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1662464, whole genome shotgun sequence.
ACCESSION   DASUTG010000198 DASUTG010000000
VERSION     DASUTG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662464"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..>2415
                     /locus_tag="VF089_04405"
     CDS             441..>2415
                     /locus_tag="VF089_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6173233.1"
                     /translation="MPLPQSSKPHRGYLESLPLSSRLDSIPLSDSEKITAQGRFFFNN
                     HEKFFLKGVTYGPFAPSTQGVPFPEPARVETDFTLMRKLGANCFRTFMPPPDWLLDMA
                     AAAGLRVIVGIPWAQHISFLDSPRTKSEVRKTIVGSVEACKRHPATFAYLIGNEIPPD
                     IVRWHGAERVRSFLGSLFKAAKASDPERFVSYANFPSTEYLEIDFTDFVAFNVYLHRE
                     QDFRRYLFHLHNLAGDRPLVLTEIGIDSVSQGLDFQAQAISWQLRVSFETGVAGTVIF
                     SWTDDWHAYSGAEGFQVDDWAFGLVDRNRQPKPAYWKAKKYYANTLPADLNRYPKVSV
                     VVCVYNGERTIDACLASLERLTYRNYEVIVVNDGSTDRTQEIAESYRYIRLINQENRG
                     LSEARNVGIRASTGDIIAFTDADCMADKDWLTYLVARFESSEFGAVGGPNLSPPDDSM
                     IASCVALSPGAPAHVLLDDEVAEHIPGCNMAFRREALEAISGFDPIFRAAGDDVDVCW
                     RLQNKGYKIGFSPAALVWHFRRNTVRDYLRQQRGYGKAETLLFFKHPSRFNVLGQSRW
                     FGRIYGDLSSFLSSRQPRIYSGVFGRGLFQTLYQPPPSVLACLPMTLEWNLLSLALLA
                     CAFIFGGWFWLGLAPFGITVARCLASALRARVPK"
BASE COUNT          518 a          654 c          632 g          611 t
ORIGIN      
        1 cgaaacccac tctgtgacga attgctctct aactttccgc gctcataaag tcggttagtt
       61 tgttgaacgc aagggtcgcc tttccatcgc ttatctttgt gttgcgtact tcacatcact
      121 gcacggacaa cacgtctgaa gcttaaagac cctcgcgtcg tcaaagacta gaacggcggc
      181 atgattcgcc gagatcgaga tgtcgaagag gtgtctatcg gtgcaggata aacctgcagt
      241 gaagtgatgc catcaaggca agataagcca gtcgaagtaa gacagacacc gtttaaacca
      301 ttgtttatta aatggaatcg tttttaggaa tttactggca tcattgttgc gttgcggcac
      361 cgttgctagc cggatagcgt tttcgcaccc tttcgtctct ctgacgcggt tttttttcaa
      421 taggagaaac accagtgaat atgccgctgc cgcaatcttc gaaacctcat cgtgggtact
      481 tggaatctct gccactatcg agccggctcg attccatccc gctaagcgac tcggaaaaga
      541 taacagcgca gggacgtttt ttcttcaaca atcatgagaa gtttttcttg aaaggagtca
      601 cctacggccc ttttgctccg tcaactcagg gagttccttt cccggagcca gcacgcgttg
      661 agaccgactt tacattgatg agaaaactcg gagccaactg tttccgcacc tttatgccgc
      721 cgccagattg gcttctcgat atggccgcag ctgccggcct gagagtcatc gtgggcattc
      781 catgggctca gcatatatcg tttcttgact cacctcgcac caagtctgaa gttcgtaaaa
      841 ctattgtcgg cagtgttgag gcttgcaagc gacatcctgc gaccttcgct tatctgattg
      901 gaaatgagat tccgcctgac atcgtgcgat ggcacggcgc ggagcgggtc cgatcgttcc
      961 tcggaagttt attcaaagcg gcgaaagcaa gcgatcccga gcggttcgtg agttacgcga
     1021 attttccttc tactgaatac ctggagatag attttaccga cttcgtggcc ttcaatgtct
     1081 accttcatcg cgagcaggac tttcgtcgtt acctttttca cctccacaat ctagccggtg
     1141 atcggccgct cgtgctgaca gagattggca tcgactcggt cagccagggg ctcgactttc
     1201 aggcccaagc gatttcctgg caactgcgcg taagcttcga gaccggtgtc gcgggtaccg
     1261 tgatcttctc atggaccgac gattggcatg cctattccgg tgctgaggga tttcaggttg
     1321 acgattgggc attcggtttg gttgatcgta accgccagcc gaaaccggct tactggaaag
     1381 caaaaaagta ctacgcgaat acgctcccgg cggatctcaa ccgctatccg aaggtttctg
     1441 tagttgtatg cgtgtacaac ggcgaacgca caatagatgc ctgcctcgct tcgttagaaa
     1501 gactcaccta tcgaaattat gaagttatcg tggtgaacga tggctccacg gacagaaccc
     1561 aggaaatcgc ggagagctac cgctacattc gtctgatcaa ccaggaaaat agaggactca
     1621 gcgaggcgcg taacgtcggg atccgggctt ccacagggga tatcattgcc ttcaccgatg
     1681 ccgattgtat ggcggataaa gattggctga cctatctggt cgctcgcttc gagtcatccg
     1741 aattcggcgc cgtcggcggt cccaatctct cgccgcctga cgattcaatg atcgcctcat
     1801 gcgtcgccct atcgcccggc gcgccggccc atgtgctttt ggacgatgag gtagccgaac
     1861 acataccggg atgcaatatg gcattccgcc gcgaagcttt ggaagcgatc tcagggtttg
     1921 atccaatctt ccgcgccgca ggcgacgatg tggatgtttg ctggcggctt cagaacaaag
     1981 gctacaagat cggcttcagc ccggcggcgc tggtttggca ttttcgtcgc aacacggtgc
     2041 gagattactt aaggcagcaa cgcggctatg gcaaggccga gactctcctg ttttttaaac
     2101 atccgagtcg atttaacgtg cttggtcaat cgcgttggtt cggcagaatt tacggcgacc
     2161 tttcctcttt cttgtcgagc cgccaaccga ggatctactc gggggtgttc ggtcgtggac
     2221 tttttcaaac gctctaccag ccgccgccct ccgtcctagc atgcctgccg atgactctcg
     2281 aatggaacct gctttctctc gcgctattgg catgcgcgtt tatttttggc ggctggttct
     2341 ggcttggact tgcgccattc ggcataaccg tagctcggtg cctcgcaagc gcgctacgag
     2401 ctcgcgtccc caagg
//
LOCUS       DASUTG010000199         9741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1069867, whole genome shotgun sequence.
ACCESSION   DASUTG010000199 DASUTG010000000
VERSION     DASUTG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069867"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VF089_04410"
     CDS             <1..935
                     /locus_tag="VF089_04410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6173234.1"
                     /translation="PKVALGANNKAYVVWSIPGGKGDKTRANIRFTMENEKGGFSPAR
                     TINEVRDAARFPIIEISPDRNVLLAWIDRRIDNPKPRQLYMMKLDSDGRELTKNYSVG
                     EGLCECCKLGVAFADEGKTVYLVDRQVDEKKIRNHVLRKSIDGGKSFAAPVLISNDEW
                     QVPSCPHSGPSIGRDTQGRLHVTWFTLGRSEKEAGIYYSVSNDDGESFAPRRLVHANT
                     APEILYSTLAVGNDDTVYLAWSNLDSYHRAQVFVRSVSADDRSWGPIQQVSNAKGNAS
                     RPVLAVTKNQLHVSWTETDGEESRVVLRSAIVAR"
     gene            1010..1519
                     /locus_tag="VF089_04415"
     CDS             1010..1519
                     /locus_tag="VF089_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6173235.1"
                     /translation="MPDVGQAQHAHPSIQNAAPTVGSPAIPFELRTLDGKAISLGTFG
                     GKPLVINFFATWCDPCREEMPLINDLAAQAATNDYSVLAVAVQDSRAAVTQYAKESKL
                     VFPIALDLNSTVKRAYRIFGPPATFFIDRQGIIRDIVIGPLTRERASTALTNAGVRKC
                     FVFYAECSK"
     gene            1564..2004
                     /locus_tag="VF089_04420"
     CDS             1564..2004
                     /locus_tag="VF089_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173236.1"
                     /translation="MQQSSYRILRVLIVGAMVMGNLFFADESLAAQSVHQHQDGRTNR
                     SERSAQAGKLLPAQGASVKILSPTKDQIFPKDQIPLEFRIVKGKRGHHVHAYVNGELM
                     GMFNSQTGTLTGLKPGKHVLELRVIAEDHKSELDAIDKVSFVVQ"
     gene            2059..2958
                     /locus_tag="VF089_04425"
     CDS             2059..2958
                     /locus_tag="VF089_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6173237.1"
                     /translation="MVLTVLRKSLVVLILFAPLALIAVHVHERGRRQTEAKDPLAVIR
                     SYLKATYARDYRAAYGYISAADQRVRDASSYAQGQGELTGFTARLARTLADFMDLKLI
                     DESSAGDRLKIKVEYSVPTPEDVSSLVWEWNSEKLNSLSTEEQIVLLDMLAARNRSGN
                     LTRIQGHETFKLIREGNAWRIFHDWAAGTKVKVQTALAGRSDVEIKLLQQEIITSGEE
                     PFQINLKINNRGSQRVVLAMRHLIEPPQATDYLEMIDCGLGRAVVLEAGTEQEFSMAY
                     LLEPSVRKNFRDLALTYAFEVKN"
     gene            2978..3559
                     /locus_tag="VF089_04430"
     CDS             2978..3559
                     /locus_tag="VF089_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6173238.1"
                     /translation="MLTAPLLAGTIAAHEPSKTKTEASRRVTSNVKMPPFTLIDQKGK
                     RFDAANKLRGKIVAINFIYTTCSDVCPLFTVEFARLQEALRSQTQLNFFLVSITTDPE
                     IDSPKVLEAYARRFGADFQNWAFLTGDESRLKDVWKGFGVQVIRRDRGLVQHTNLTTL
                     VDAQGIRRINFLGPQWQFSDFLKDMRALSPNPP"
     gene            3646..4227
                     /locus_tag="VF089_04435"
     CDS             3646..4227
                     /locus_tag="VF089_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6173239.1"
                     /translation="MKLLHRLSLAAILVGVTLSSFQYARYGVFGSIANAQDLESKKIV
                     PFVPTPQEVVDKMIELAGIKKGDVVYDLGSGDGRIVITAAKKGARAVGFDVDGDLVKE
                     SRENIRKAGLEKLAEIKQQDILTVDLSPATVVTMYLLPDVNLKLKPNLLKQLKPGSRV
                     VSHAFDMGDWKPDKTERVNGRQIYLWTIPAKSP"
     gene            4286..5185
                     /locus_tag="VF089_04440"
     CDS             4286..5185
                     /locus_tag="VF089_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6173240.1"
                     /translation="MRRKFLSQRSYSSLFVIALLALSSLHMRSAAAADQQDSVSTLDR
                     YVRATYARDYEEAYSLIATADQRLKDRATYVRDRGAFTGFTLEIARVLASYIQLKPSE
                     TRIVGGQARINIKFEVPDAGKLAPMLHDWNIEQLEGLSVTERKALLERIEKLGRNHAI
                     EMVQGEDVFELVQEGASWKIFLNWAAPVNIGFRTSVPSSVPLVARLEHSDVATRPGEI
                     FKVVLNVKNTSHKQLFARIGHLVDPHGVRDYLDLVECGFLLPVRLAPGKEEEFVSTYL
                     LRKNFPEGVRQLNVTYAVTLGNN"
     gene            5270..6169
                     /locus_tag="VF089_04445"
     CDS             5270..6169
                     /locus_tag="VF089_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6173241.1"
                     /translation="MDSRMTSWFSGVAAALILILPAAFLYSNSPPEHSHQTEPFNTVE
                     AYLKNSYARDFKKAYRYVSRGDRQLKSERVYVREKGSFSGFPLTVTRKLAQFIELRPL
                     ETAREQDGQMRVKVEAKYPDANSLSAILLEWDEERLNALPTSMQRDILSKLDQMDRAG
                     EIKTIEGEEEFTLVKEGTSWRVYLDWAAGVRVSYDAAVPTESIEAIPVYKSTTIRPNE
                     LFTISYRVKNRTAQTISTRITHRIEPDELSQHLDIIDCALLLPVELRPGEESEFSTTY
                     MVRGDLPEGTKELNVTYDFQVAH"
     gene            6138..6770
                     /locus_tag="VF089_04450"
     CDS             6138..6770
                     /locus_tag="VF089_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6173242.1"
                     /translation="MSPMTFKSRISLWIAAVSLAVGLLSTPVFSHIPTPPKKGEIGRR
                     ESSTPAPNFALIDQSGKPFKFTPASGKLVLVTFVYTTCPDVCPLFTAKLASIQRILEQ
                     EKRDDYLLLTITTDPARDTPAKMKAYAQAFHADFRRWHFLSGTREALAPVWKEFGVTV
                     KDLGNGQIQHTNLTTLIDPKGIRRVDYYGDKWQEKEILKDIERLRQARPQ"
     gene            6767..7324
                     /locus_tag="VF089_04455"
     CDS             6767..7324
                     /locus_tag="VF089_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX6173243.1"
                     /translation="MMRSLAIFSTLLLLVLWAGSGVAAPEQGIFEVRIKDHREAIDDF
                     SRLILTFDKVAISPKPGLKFWQTSWKEFSASQASVDLTKYIGKDSAKIFRADIDVGAF
                     DAFHLKIKSLEGFLKKTQLNAPVKNTIGPVKLSFDIAPRGETVLVLDLTVIDFSDHPP
                     RGYELSLKGYELYRNGKLVDKIPPG"
     gene            complement(7379..8092)
                     /locus_tag="VF089_04460"
     CDS             complement(7379..8092)
                     /locus_tag="VF089_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6173244.1"
                     /translation="MNLRWSVLRIPMRSMLEARTLIALFITVSAMLFFAAFVDRVVEG
                     DTRAFDESLLLAFRDPNNPSYTIGPAWLQIMFRDITSLGGYAVVMLISLAVMGYLLMD
                     GKYGAALWVLASVGGGAVLSNVLKLAIERPRPDLVARLVEVNTTSFPSGHATLAAVTY
                     LTLGALLSRVEARRGAKIYVLTVAVVLTFLIGVSRIYLGVHWPTDVLAGWCVGAAWAL
                     LCWRIALALQRSGRIESDV"
     gene            complement(8219..8431)
                     /locus_tag="VF089_04465"
     CDS             complement(8219..8431)
                     /locus_tag="VF089_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173245.1"
                     /translation="MAIFDSLLNALRALAVGDRVRITVRPETGVFPNPVDGEITAKDD
                     SGNLALKSQQALIQVSAGDILSITKL"
     gene            complement(8496..9695)
                     /locus_tag="VF089_04470"
     CDS             complement(8496..9695)
                     /locus_tag="VF089_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6173246.1"
                     /translation="MQPTSKPGAATRGIGTIAVHGGEARPKLGNALATPIVQTATYTF
                     ANTDELRDHFERRIEREEYGRYGNPTQRIAEQKLAELEGAEDCLLFASGMAAITTTLF
                     AVLSHGSHVVVTDDSYRRTRQFLTQVLHRYGIEVSVARAGDYEAIEGAIRPTTRVLVS
                     ESPTNPYNYIVDLERFSEIGRRHRVKTVIDATFATPFNQRPLEFGIDLVLHSATKYLG
                     GHNDLLAGAVLGSADLIDGIRGLQAVTGAIADPFTAYLLIRGLKTFALRIARQNATAQ
                     ALAEFLQSHPRITAVYYAGLASHPQHNVARRQMRGFGGVVSFEVAGDLDATSRVVDAC
                     RIPQIAPSLGGVESLIEQPALMSFYELTTEERLLVGIKDNLIRYSVGIEDSDDLIADL
                     TAALERI"
BASE COUNT         2401 a         2582 c         2590 g         2168 t
ORIGIN      
        1 gacccaaagt cgcattgggg gcgaacaaca aagcttatgt cgtttggtca atacccggag
       61 gaaaaggcga taagactcgc gccaacatcc gcttcacaat ggaaaacgaa aagggcggct
      121 tcagcccggc ccgaaccatc aacgaagtta gagatgcagc gcgttttcca atcatcgaaa
      181 tatcaccgga tcgtaacgta ttgctggcct ggatcgatcg tcggatcgat aatcctaaac
      241 cgcgccagct atacatgatg aagctcgatt cggatggaag agagttgacg aagaactatt
      301 cagtaggtga aggtttgtgc gagtgctgta agctcggcgt cgcctttgcc gatgaaggga
      361 agacagttta cctggtcgac cgccaggtag atgagaaaaa aattcgcaac catgtgctgc
      421 gtaagtccat agatggcggc aaaagcttcg ccgcgccggt cctgatcagc aacgatgaat
      481 ggcaagttcc ctcctgcccg cactctggcc ccagcatcgg acgcgatact caaggtagac
      541 ttcacgtgac gtggttcacg ctcggtcgtt cggagaaaga ggccggtatt tattacagcg
      601 tgtctaacga tgacggcgaa agtttcgcgc cgcgccgttt ggtgcatgcc aacaccgctc
      661 cggaaattct ctactcaact ctggccgtgg gaaacgatga caccgtctac ctggcttgga
      721 gcaacctgga tagctatcac agagcgcagg tttttgtgcg ctctgtatca gccgatgacc
      781 gctcctgggg acctattcag caggtgagca atgccaaggg caacgctagc cggcctgttt
      841 tggccgtgac gaaaaatcag ctgcacgttt cctggaccga aacagacggc gaggaatcac
      901 gggttgtcct cagaagcgcg atcgtggcaa gatgaaccag aaattatccg ctatgaagct
      961 gtgtgccggt agagcatggt tatgcgcaat cgttcttgca tttgcactga tgcccgacgt
     1021 aggacaagcc cagcacgctc acccatctat ccagaacgct gcgcctacgg tcggctcgcc
     1081 ggctatccct ttcgaattgc gaacactcga tggaaaggcg attagcctcg gaacctttgg
     1141 cggcaagcca ttggtgatca atttcttcgc tacctggtgc gatccatgtc gggaagagat
     1201 gccgcttatc aatgatctgg cggcacaggc ggcaaccaac gattacagcg tcttggcagt
     1261 ggcggttcag gatagccgcg cggcagtgac acaatacgca aaagagtcta aacttgtttt
     1321 tcctatcgca ctggacttga atagtacggt gaagcgggcc tatcgaatct tcggcccgcc
     1381 ggcaaccttt tttattgacc gccagggcat catccgcgac atcgtcatcg gcccgcttac
     1441 acgggaacgg gcatcgacag cgcttacaaa tgccggtgta cgaaaatgtt tcgtttttta
     1501 cgccgaatgc tccaagtaac cgcttgtccg ggttggcgtc gtgatgagct aagggaaata
     1561 tgaatgcaac aatcaagtta ccgtatatta agagttctaa ttgtcggcgc tatggttatg
     1621 ggaaatcttt tctttgctga tgaatcactc gccgcccagt cggttcacca gcatcaagac
     1681 gggcggacaa accggtcgga gagaagcgcg caagccggca agctactacc ggcgcaggga
     1741 gccagcgtga aaatattgtc ccccacaaaa gaccagattt ttccaaagga tcaaattccc
     1801 cttgagttta gaatcgtcaa aggaaaacgg ggtcatcatg tgcatgccta tgtgaatggc
     1861 gaactcatgg ggatgttcaa cagccaaaca gggactctca ctgggttaaa gcctgggaag
     1921 cacgtcttgg agttacgcgt cattgcagag gatcataaaa gcgagctgga tgccatcgac
     1981 aaagtttctt tcgtcgtcca ataggttttt cgagtgagat caaaattacc gccgatacaa
     2041 attttgtgtt cctttcctat ggttcttact gtactgcgca agagcctcgt cgtattaatc
     2101 ctgtttgctc cgctggcttt aattgcagtt catgtacatg agcgaggccg tcgacagacc
     2161 gaagcgaagg acccgctcgc ggtaatccgt tcttatctta aagcgactta cgcgcgcgat
     2221 tatcgggctg cctatggcta tatttcagcg gcggaccaga gggttcgtga tgcaagcagc
     2281 tatgctcaag ggcaaggaga gcttaccgga tttacagctc ggctagcgcg tacgctcgct
     2341 gatttcatgg acttgaagct catcgacgaa tcctccgccg gcgaccggtt aaaaatcaaa
     2401 gtcgagtact ctgttcccac gcccgaagat gtttcatcgc tggtctggga atggaattca
     2461 gaaaagctca attcgctatc aacggaggag cagatcgtat tgcttgatat gctggctgca
     2521 cgaaaccgga gcggcaatct gacccggatt caaggacatg agacattcaa gcttattcgt
     2581 gagggaaatg cctggagaat atttcacgat tgggccgccg gaacgaaagt caaggtgcag
     2641 acggcgttgg ccgggagatc agacgttgag atcaaacttt tacagcaaga aattatcacc
     2701 agtggggaag agccttttca aatcaacttg aagattaaca atcgcggaag ccagcgtgtg
     2761 gttctggcca tgcgccactt gatcgaaccg ccccaggcca ccgattatct ggaaatgatc
     2821 gactgtggtc ttgggcgtgc tgtcgtgctc gaagcgggaa ccgagcaaga gttctcgatg
     2881 gcttatctgc ttgaaccatc ggtccggaag aacttccgag atctggcact cacctatgcg
     2941 tttgaagtta agaattagct ctctcgtcgg attcatcgtg ctaactgccc cattgcttgc
     3001 cggcaccatc gcggctcatg aacctagtaa gacgaaaaca gaagcgtccc gccgggttac
     3061 ctcgaacgtg aagatgcctc cgttcacgtt gatagatcaa aaaggtaagc ggtttgatgc
     3121 ggcgaacaaa ctacgcggca aaatcgtcgc cattaatttc atctatacga cctgctccga
     3181 cgtttgcccg ttattcacgg tcgaatttgc tcgcctccaa gaagcgctcc gcagccagac
     3241 tcaactgaat ttcttccttg tgagcattac cactgacccc gaaatcgatt cgccaaaagt
     3301 attggaggcg tatgcacggc gattcggcgc cgatttccag aactgggcat tcttaactgg
     3361 cgatgagtca cgcctgaaag atgtttggaa aggattcggt gttcaggtaa tccggagaga
     3421 ccgtgggctg gtgcaacaca cgaatttaac gaccttggtc gatgcccaag gcatccgcag
     3481 gatcaatttt ctgggtccgc aatggcagtt cagcgatttt ttaaaagaca tgcgagcgtt
     3541 gagcccaaac cctccctgat ctcggttggc gagatagtgg cgactcgatt ttttttatgt
     3601 ttaattgttc cggacaccca ttgatcacga aagggaggct ataaaatgaa actgctgcat
     3661 cgcctttcac tggctgcaat tctggttggg gttaccctct catcgtttca gtatgcgcgt
     3721 tacggagtct tcgggagtat tgccaatgcc caggatctcg aatctaagaa aatcgtgccc
     3781 tttgtaccca cgccgcaaga ggtggtcgac aaaatgatcg agctcgccgg tataaagaaa
     3841 ggagacgtgg tctacgatct tggctctggc gacggcagaa tcgtcatcac cgccgccaag
     3901 aagggcgccc gcgcggtagg gttcgacgtt gacggggatc tcgtcaagga gtcgcgggag
     3961 aacattcgca aagcgggttt ggaaaaattg gctgagatta agcaacaaga catcttaact
     4021 gtagatctct cgccggccac agtagtgacg atgtaccttt tgccggatgt taacctgaag
     4081 ctcaagccga atctcctgaa acagcttaaa ccgggttccc gggtggtctc tcacgccttc
     4141 gatatgggtg attggaaacc tgataaaacc gagcgagtca acggccggca gatttatctt
     4201 tggaccatcc cggccaaatc gccttaaccc gatgtaggtt atttggtgac cactcgttgt
     4261 gcagttactt cggaattgtc atcgcatgcg aagaaaattt ttgagtcaaa ggtcctattc
     4321 cagtttgttc gtaatcgcgc tcctagcgct ttctagcctc cacatgcgtt ccgccgctgc
     4381 tgccgaccaa caggactcgg tcagcacgtt ggaccgctac gttcgggcaa cctacgccag
     4441 agactatgag gaagcttatt cccttattgc cactgccgac caacgtctaa aagatcgcgc
     4501 cacctatgtc agagaccgcg gcgcatttac cggattcaca ttagagatcg ccagagtact
     4561 tgcgagctat atacaactaa aacctagcga gacccggatc gttggcggac aagcacggat
     4621 caatatcaag ttcgaagtgc cggacgcggg aaagctagct ccgatgctgc acgattggaa
     4681 tatcgagcag ctggaagggc tgtcggtcac cgagcggaaa gcgttgctgg agcgcatcga
     4741 gaagctggga cggaatcacg cgatcgagat ggttcaaggg gaggatgttt tcgaactggt
     4801 ccaagagggc gcttcctgga agattttttt gaattgggca gcgccggtaa acatcggctt
     4861 tcgaacctcg gttccgagtt cagtcccact ggtagcgcgt ctcgaacatt ccgatgtggc
     4921 aacccgaccg ggtgaaatct tcaaagtcgt gctcaacgtg aaaaatacca gtcacaaaca
     4981 gcttttcgct cgcataggcc acttagtcga tccccacgga gttcgcgatt atctggatct
     5041 tgtcgagtgc ggtttccttc tgccagtgcg actagcacct ggaaaagaag aagaattcgt
     5101 cagcacctac cttctcagaa aaaactttcc cgagggagtg cgtcagctaa acgtcaccta
     5161 tgccgtcact ctgggaaata actaatcagc tctttgcccg attctgggtc cgcggcttct
     5221 caccgcagag ttaacgaaat ggagcccttg atagatcagg aagtattgga tggattcacg
     5281 aatgacaagt tggttttcag gtgtggcagc ggcattgata ctgattctgc cggccgcttt
     5341 tttatatagt aacagcccgc ccgagcacag ccatcagaca gagccgttca acaccgtcga
     5401 ggcttatctc aagaattcct acgcgcgcga tttcaaaaaa gcctatcgtt acgtctcccg
     5461 cggcgaccgg cagctcaagt ctgagcgcgt gtacgttcgc gaaaagggat ccttcagcgg
     5521 ttttccgctt accgtgacac gtaagctggc gcaattcatc gaattgcggc cgctggaaac
     5581 ggctagagaa caagacggcc aaatgcgggt caaagttgaa gctaaatacc ccgacgcgaa
     5641 tagcttatcg gccatcctgt tggaatggga cgaggagcga ctgaacgctt tgcccacatc
     5701 gatgcaacgc gacattctat ccaagcttga tcagatggat cgcgcaggtg aaatcaagac
     5761 gatcgagggt gaagaagaat ttactttggt taaggaaggc acaagctggc gggtttatct
     5821 cgattgggct gccggggtca gggtgagtta tgacgcggca gtgccgacag agtctataga
     5881 agcgatcccc gtctataaat ctacgacaat ccgcccaaac gaacttttca ccatctctta
     5941 ccgagtcaag aaccgaacgg cgcagacgat ttcgactcgg atcactcatc gaatcgagcc
     6001 cgacgagctc agccagcacc tggatatcat tgattgcgcc ttgctgcttc ccgtggaact
     6061 acgccccgga gaggagtcgg agttttcgac tacttatatg gtgcgcggcg atcttcccga
     6121 aggaacaaaa gagttaaatg tcacctatga ctttcaagtc gcgcattagc ctatggattg
     6181 ccgcagtgtc cctggcggta ggcctgctgt caacgccggt attttcacat atccccactc
     6241 caccaaaaaa aggcgaaata gggcgtaggg agtcgagcac cccggcgccg aactttgccc
     6301 ttatcgatca atccggcaaa ccctttaagt tcactcccgc cagcgggaaa cttgtcctgg
     6361 tcacgtttgt ttacaccacc tgcccggacg tttgtcccct gttcacagcg aaattggcgt
     6421 ccatacagag aattctagag caggaaaaac gtgacgatta ccttcttttg accatcacca
     6481 ccgatcccgc gcgcgacact ccggcaaaga tgaaagcata cgctcaagct ttccatgcgg
     6541 attttcgacg ctggcatttt ctcagcggca ctcgcgaggc gctggctcca gtttggaaag
     6601 aattcggcgt gacggtcaaa gatctgggca acggccaaat tcagcacacc aacctaacca
     6661 cactcatcga tccaaaaggc attcgccgcg tcgactatta cggcgacaaa tggcaggaaa
     6721 aagagatcct aaaagatatt gagcggctac gccaggctag gccgcaatga tgagatcgtt
     6781 ggctatcttt agcacgctgt tgctgctggt gctctgggcc ggctccggcg tggccgcgcc
     6841 agagcaagga atctttgaag ttcgtatcaa ggatcaccgc gaggcaatcg atgatttttc
     6901 tcggttgatc ctgacttttg acaaagtcgc aatcagtccc aagccgggac ttaaattctg
     6961 gcagaccagc tggaaggaat ttagcgcttc tcaagcctcg gtagatttga cgaaatatat
     7021 cggtaaagac agcgctaaaa tattccgggc ggatatcgat gtcggagcat tcgatgcatt
     7081 ccacctaaaa ataaagtcgc tcgagggttt ccttaagaaa acccaactaa acgcgccggt
     7141 gaaaaatacg attggaccgg tcaaactgtc gttcgatatt gcaccacgcg gagaaacggt
     7201 cctcgtgctc gatttgaccg tcattgactt ctccgaccat ccgccgcgtg gatacgaatt
     7261 gagtctcaaa ggctatgagc tttatcgtaa tggcaaattg gttgataaga ttccacccgg
     7321 ataagatgct aaatcttatc cggcacgcag ttctgaatct ggttagcttc tgaatgaatc
     7381 agacgtcgct ttcaatcctt ccacttcgct gcagcgcaag cgcgatgcgc cagcataaca
     7441 atgcccaagc ggcaccaacg caccagcccg ccagtacatc tgttggccag tgaacgccga
     7501 ggtaaatgcg gctgacaccg atcaagaagg tcaggaccac tgccactgtc aggacataaa
     7561 tcttggcacc gcgacgcgcc tccactcggg agagcagcgc tcccaaagtg agataagtca
     7621 cagctgcaag ggtggcgtgg ccgcttggga aacttgtggt atttacctcg actaggcggg
     7681 caacgagatc cggtcgcggc cgctctatcg caagcttaag tacgttgcta agcacggcac
     7741 ccccgccaac cgaggccagc acccacagtg cagcgccata tttaccgtcc atgagcagat
     7801 aacccatcac agctagagaa atcagcataa cgacggcata gccgccgagg gaggtgatgt
     7861 cccgaaacat gatttgtagc catgccgggc cgatagtgta agaaggattg ttcggatcgc
     7921 ggaaagccag caatagactc tcgtcaaaag cgcgggtatc cccttcaacg acgcgatcga
     7981 cgaatgcagc gaaaaagagc atcgcgctga cggtgatgaa tagcgcgatc aaagttcgtg
     8041 cctccagcat cgagcgcatc ggtatccgca aaacggacca gcgtaagttc ataaactcac
     8101 gctcagtcta tcacctccgg agctttttct acgatctgcg agacgctgct tcgggatgag
     8161 gcccggatcg cagtggcggt gatacacggc gaagggggtt cagtttcggc gattacaatt
     8221 atagtttagt aatcgataga atatcgccag cactcacttg aatcagtgcc tgctgcgact
     8281 tcaaggcaag atttccgctg tcatccttag cagtgatctc tccgtccact ggatttggaa
     8341 aaactccggt ttcgggccga accgtaatcc ttacccgatc accaaccgca agcgccctca
     8401 gcgcgtttag caacgaatcg aatatcgcca tcacacctcc gggacatcaa tttgagaaca
     8461 cgatcgagtt tcgatctgaa atcagcgcat gtgattcata tgcgttcgag ggcagcagtc
     8521 aaatcggcga tcaagtcatc cgaatcttcg atgccgaccg agtagcggat gagattgtct
     8581 ttgatgccga cgagtaggcg ctcttccgtc gtaagctcgt aaaagctcat cagcgcaggc
     8641 tgctcgatca aactctccac gcctcctaac gagggcgcta tctgcggaat gcggcaggca
     8701 tcgacaacgc gcgaggttgc atcgagatcg ccggcaacct caaacgagac gacaccgccg
     8761 aaaccgcgca tctggcggcg cgccacattg tgctgcgggt gcgatgctag accggcataa
     8821 tacacggcag tgatccgcgg gtgcgactgg aggaattcgg cgagggcctg cgcggtcgca
     8881 ttctgccggg caattcgcaa tgcaaatgtt ttgaggcctc gaataagaag ataggctgta
     8941 aacggatccg cgatcgctcc ggttaccgct tgcaagccgc gaatgccatc aatgagatcc
     9001 gcggatccaa gcaccgcacc agcgagcaga tcgttatgcc cgcccaggta tttggtcgcg
     9061 gaatgaagaa ccagatcaat gccgaactcc agcggccgct ggttaaacgg cgtcgcaaac
     9121 gtcgcatcga taaccgtctt tacccgatgg cgtcggccaa tttcgctgaa gcgctcgaga
     9181 tcgacgatgt agttgtaagg attcgtcggc gattcgctta cgagcacgcg tgtggtcggc
     9241 cggatcgctc cttcaatagc ctcgtagtcg ccggctcgag ccaccgagac ttcgatgcca
     9301 taacggtgca acacctgagt gagaaactga cgggtccgtc gataagaatc atctgtgacg
     9361 acgacatggg atccgtgcga gagcacggca aacagcgtcg tcgtgattgc ggccataccg
     9421 ctggcaaaca gtaaacaatc ctcggctccc tcgagctccg ccagcttctg ctcggctata
     9481 cgttgcgtcg gattgccata acggccgtat tcctcgcgct cgatacgtcg ctcaaaatgg
     9541 tcgcgaagct catcggtatt tgcaaaggta taagtggcgg tctgcacgat cggtgtcgcc
     9601 agcgcattgc cgagcttcgg ccgcgcctca ccaccatgca ccgcgatagt accgatgcct
     9661 cgggtcgctg cgccaggctt tgacgtcggt tgcacccccg tctgtgtctg cagcggcaag
     9721 gtggcgggtt tgtcgctgtc c
//
LOCUS       DASUTG010000200         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_33004, whole genome shotgun sequence.
ACCESSION   DASUTG010000200 DASUTG010000000
VERSION     DASUTG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33004"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VF089_04475"
     CDS             complement(<1..1256)
                     /locus_tag="VF089_04475"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6173247.1"
                     /translation="MFAKSVLENGIRVVSQEMTDHRSVSLGIWVENGSRHELPDENGI
                     SHFIEHLLFKGTTTRTAAQIAEEMDAVGGLLNAFTSKEHTCYYAKVLDEDLPLASDLL
                     SDIFLNSNFDGEEIERERSVILQEISQAEDTPDDYVHDLFSLDFFQNHPLGRPIAGHV
                     GTVSKFRREDFVNFFHERYHPSRVVVSAAGHFQHDKLVKELNARLGGVKKSVSAVASR
                     VDAELLPQTKSGVFTHAKPLEQVHLCLGLPGIHQTHPQRHAAYILNTILGGGMSSRLF
                     QEIREKRGKAYSVYSFMSTYSDTGYLGIYAGTSLEAVEEVIDLVLRELKCLAAGEIKE
                     DEVRRTRGQLVGNIMLGLESTDSWMSHIARNELYFGKSITTDEICQRIRSVTRDEIIE
                     LARALFRPETMAVSLLGDFKEDFKIK"
     gene            complement(1280..>2419)
                     /gene="pnp"
                     /locus_tag="VF089_04480"
     CDS             complement(1280..>2419)
                     /gene="pnp"
                     /locus_tag="VF089_04480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6173248.1"
                     /translation="PRTHGSALFTRGETQALVVTTLGTASDEQRVDALIGEHFKKFML
                     HYNFPPFSVGEVKFLRGPGRREIGHGNLAERALLPVLPPEEGFPYSLRIVSEILESNG
                     STSMASVCGGSLSLMDAGVPVSAPVAGIAMGLIKEGEHVRILSDILGDEDHLGDMDFK
                     VAGTAEGVTSLQMDIKVSGVDREIMRQALYQAKEGRMHILGEMGKTLSAPRTNVSGHA
                     PRIITIRVRPEKIREIIGPGGKVIRGIIEATGVKMDVEDDGTVTIASSDEAAAQQAIQ
                     MVQRIAAEAEVGKIYKGTVRKIVDFGAFVEIFPGTDGLIHISQLAPERVRRVADVLKE
                     GDEVMVKVLEVDRQGKIRLSRKEALQESSGGQTNLGGGSDGAPEE"
BASE COUNT          560 a          673 c          605 g          581 t
ORIGIN      
        1 tttttgattt tgaaatcttc cttgaaatca cccagcaggg aaactgccat tgtctcgggc
       61 cgaaacaagg cgcgcgccaa ttcgatgatt tcatcacggg tgacagaacg aatacgttga
      121 cagatctcat ctgtggtgat cgatttgccg aagtaaagct cattgcgagc gatatgactc
      181 atccaggagt ccgtggattc caagcccagc atgatatttc ccaccagttg ccctcgggtg
      241 cggcgaacct cgtcttcttt aatctctccg gctgccaaac attttaactc cctcaacacg
      301 agatcgatga cttcttcaac ggcttctaga ctggtgcctg catagatgcc gagatagccc
      361 gtatcgctgt aggtggacat gaaagaataa acagaatagg ccttgccgcg cttctcacga
      421 atctcctgaa acaatcgaga actcatgcca cccccgagta tggtgttcag tatataggcc
      481 gcgtgtcgtt gcggatgtgt ctggtgaata cccggcagac ctaaacatag atgaacctgt
      541 tctagaggct tagcatgggt gaacactccg cttttggttt gcggcaagag ctcggcatcg
      601 actcgacttg caacggctga aacagatttt tttacgccac caagacgcgc attcaactcc
      661 ttaaccagct tgtcatgttg gaagtggccg gcggccgaaa caacgactct gctcggatgg
      721 tatcgttcgt gaaagaaatt gacgaagtct tcccgacgga atttcgagac agttccaaca
      781 tgaccggcaa tcggccggcc taatggatga ttctggaaaa aatctagact aaagaggtcg
      841 tgcacatagt cgtccggagt atcttcagcc tgggaaatct cttgaaggat aaccgatctt
      901 tcacgctcga tctcttcgcc gtcgaaattc gaattgagga aaatatcgga cagcagatcg
      961 ctcgccagcg gcagatcttc atcaagcact ttggcgtagt agcaggtgtg ttccttggaa
     1021 gtaaaggcgt tcagcaggcc tcccacggca tccatctctt cggcgatctg cgcggctgtg
     1081 cgtgtcgtcg ttcccttgaa aagcaaatgt tcgatgaaat gggatatgcc attctcatcc
     1141 ggcagttcat gacgcgaacc attctcaacc catatcccta agctcaccga acggtgatcg
     1201 gtcatctctt gagagacaac tcgaatgcca ttttccaaaa cgcttttagc aaacatcgat
     1261 acgatacgga gctggagggt tactcttcgg gagcgccatc tgagccgcct ccgaggtttg
     1321 tttgtccgcc gctgctttct tgaagagcct cttttcgact gagcctaatt ttaccctgcc
     1381 gatccacttc aaggactttg accatgacct catcgccctc tttcagcacg tccgcaacac
     1441 gtcgcacgcg ttcaggagca agttgcgaaa tatgaatcag cccatcggtc cccgggaaaa
     1501 tctcgacgaa ggcaccgaaa tcgacgattt ttcggacggt tcctttgtag attttgccaa
     1561 cctccgcttc ggctgcgata cgctggacca tttgtatggc ttgttgtgca gcggcttcat
     1621 cgctcgaagc gatggtaacg gtgccatcat cttcaacatc catcttcacc ccggtggctt
     1681 caatgatccc acggataact ttgccgccag ggccgataat ttcgcggatc ttctcgggtc
     1741 tgactctgat ggtgatgatt cgcggcgcat ggcccgaaac gttcgtgcgg ggcgccgata
     1801 aagtcttgcc catctcgccc aggatgtgca ttcgaccttc tttagcttga tagagcgcct
     1861 ggcgcatgat ttcacgatcg acaccgctga ctttgatgtc catctgtagc gaagtcacac
     1921 cctcagccgt tcctgcaact ttaaaatcca tatcccccag gtgatcctcg tcaccgagga
     1981 tgtccgagag gatgcgaacg tgctcgcctt ctttgattag ccccatggcg atgccggcga
     2041 ctggcgcgga aaccggcaca cccgcgtcca ttagggaaag cgatccgccg cagaccgacg
     2101 ccatcgacgt tgacccattt gattccaaga tttccgaaac gattcgtaat gagtacggga
     2161 agccctcctc cggtggtaga accggcagca aagcacgttc ggcgagattg ccatgaccaa
     2221 tctcgcgccg gcccgggccg cgcaggaact taacttcacc tacactgaag ggcgggaaat
     2281 tgtaatggag catgaacttt ttgaagtgct cgccaatcaa agcgtcaacc ctttgctcat
     2341 cagaagcggt gccgagcgtc gtcaccacca gcgcttgagt ctcgccacgt gtaaacaggg
     2401 cggacccgtg ggtgcgcgg
//
LOCUS       DASUTG010000201         7286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1663144, whole genome shotgun sequence.
ACCESSION   DASUTG010000201 DASUTG010000000
VERSION     DASUTG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663144"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1372
                     /locus_tag="VF089_04485"
     CDS             398..1372
                     /locus_tag="VF089_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6173249.1"
                     /translation="MKIAVIDDYQNAFRTLKCYPKLRDHEVVVFTDTETDIGKLADRL
                     KDADAVVLTQQRSSFPRALIERLPNLKLIGQTGRAATHVDLDACTEKGIVVSAGGAGN
                     SSATAELTWGLILSALRNLPFEVKRLKEGHWQSTLGIGVNGKTLGIYAYGKIGSIVAA
                     AGKAFGARVVCWGREGSTGRAKAAGFEVAQSREDFFAEADILSLHLPLNKDTQGIVTR
                     DDLGRMKPTALIVNPSRAGLIAPGALVEALKAGRPGMAAVDVYEKEPVIGADHPLLRM
                     ENVTCTPHLGYVTREGYEDYYAVVVDDILAFAAGKPQNVLNPPALAKK"
     gene            1705..1947
                     /locus_tag="VF089_04490"
     CDS             1705..1947
                     /locus_tag="VF089_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173250.1"
                     /translation="MQIQIELTEESRELLMRHFKETASLHEVLARAQRNELAGVNVYS
                     FECDLQQATELLEMARDHCPPAVKDIEHAIFAATAG"
     gene            complement(2304..3827)
                     /locus_tag="VF089_04495"
     CDS             complement(2304..3827)
                     /locus_tag="VF089_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6173251.1"
                     /translation="MLAKINSAALYGIDALRVEVEIDLASGLPQLATVGLPEGAVKES
                     KDRIRAALKNCGYTFPAKRITINLAPADIKKEGSAYDLPMAIGILAAEGSVDKNLIND
                     YFLIGELSLDGAIKPVRGALPIAMAAKREHVKGLILPKHNAPEAAVVSEVEVLGVSDL
                     PEVVEFLNGRRAIERTHVNLEALFRQQSQYSSDFTDVKGQQHVKRALEVAAAGGHNVI
                     MVGPPGSGKTMLANKLPTILPDLTLAEALETTKIHSVMGLLNGSPIIARRPFRYPHHT
                     ISDAGLVGGGTVPKPGEVSLAHNGVLFLDELPEFKRNVLEVMRQPLEDGSITVSRAIG
                     SINYPAGFMLVAAMNPCPCGFFTDPQKECVCTPLQIQRYRSRVSGPLLDRIDIQVEVP
                     ALRYQDLASKDAGESSDSIRQRVNAARAIQLQRFLKRHIHGNAQMGARDIKRHCQLKD
                     DAEKLLENAINRLGLSARAYSRVLKVGRTIADLAGSEEIQSSHIAEAIQYRSLDRRM"
     gene            complement(3900..4133)
                     /locus_tag="VF089_04500"
     CDS             complement(3900..4133)
                     /locus_tag="VF089_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6173252.1"
                     /translation="MPLYEYECSKGHHFEAIQKVSDKPLRRCMICAAAAKRALSRPTL
                     LHNRGVHVFDRVTKDDALRMRPSSLKSFKKDKF"
     gene            4302..5768
                     /gene="guaB"
                     /locus_tag="VF089_04505"
     CDS             4302..5768
                     /gene="guaB"
                     /locus_tag="VF089_04505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX6173253.1"
                     /translation="MLEQEVLPVGLTFDDVLLIPAESAVLPRDTDVSTRLTERITLNI
                     PLVSAAMDTVTESKTAIAMAQEGGIGIIHRNLPVGIQAAEVERVKKFESGMISDPITV
                     APDQQISEAKAIMDKYGISGLPVKRNGRLVGILTNRDLRFEKKLDRTVSEVMTKDKLI
                     TVKEGVDLDQAKELLHRHRIEKLLVVDDDFNLKGLITVKDIEKRTQYPNACKDTRGRL
                     RVGAAVGVGPDWEERVEALVRAGADLIAIDTAHGHSRNVLDTIRQIRGRYPDLDLSGG
                     NVATAEGTTALIQAGVNVVKVGVGPGSICTTRVVSGVGVPQVTAIGSCAKAAARHNVP
                     IVADGGIKFSGDITKALAAGADSVMIGSLFAGTEESPGETILYQGRTYKVYRGMGSLG
                     AMKQGSKDRYSQSDVEETMKLVPEGIEGQVPYKGSLSINIHQLVGGLRAGMGYLGCRD
                     VATLRSKARFMQITAAGLREGHVHDVFITKEAPNYSVE"
     gene            5765..>7286
                     /gene="guaA"
                     /locus_tag="VF089_04510"
     CDS             5765..>7286
                     /gene="guaA"
                     /locus_tag="VF089_04510"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX6173254.1"
                     /translation="MILVLDFGSQYTQLIARRIRESNVYCEIHPFSVGPDKISELNPE
                     GIILSGGPASVYQDNAPKVNPEIFDLPVPFLGICYGMGVMNLASGGAVARADHREYGP
                     ADLMIDSDADLFYGFKKNDPVRVWMSHGDKMTAVPTGWSRIAHSGNSPIAAFADPTRR
                     FFGVQFHPEVAHTPRGKEILDNFIFRICGCKPDWTMEHFIETSVRKIRDQVGSGRVLC
                     GLSGGVDSSVAATLIHRAIQERLICVFVNNGLLREGEAERVCQLFSERFGSSFRYVDA
                     SEAFLSDLSGIEDPEIKRKKVGYKFIEVFENEAEKLGEIDFLAQGTLYPDVIESVSFV
                     GPSVTIKSHHNVGGLPSVMKLKLIEPLRELFKDEVRTLGKELGLPHEMIHRQPFPGPG
                     LAIRILGPVTEERLKILRAADTIVWHEMRAAGLYEKVWQSFAVLLPIKTVGVMGDERS
                     YENVIALRVVESLDGMTANWVPLPADLLSRISNRIINEVRGVNRVVYDISSKPPATI"
BASE COUNT         1668 a         1882 c         2076 g         1660 t
ORIGIN      
        1 accataacgt atgactttat agcagtaaag catcgccatc accgatgagc ttaacttaaa
       61 tagccgccgg ctggttgatt tcccctggtc acctaaatta agagcagcga ccggatgctg
      121 gtcagattgc ttgccgcccg ccagcagcga tgctaaagac atagcgagct tgacgctcgt
      181 ctgtttgttg gcgtgagcga atcgtaaagg atgatcgtcg agggctatca acctgatcta
      241 ccagctgcca aaaactgtca tccaagtgaa accgttttcc gacggacgag gtaaggagcc
      301 gagtagttcg attcgagacc gatcatttgg caccagtatc tttcagccca agtgaatctc
      361 ttaccaggca cagcgaattg atgaaaggta gcttcctatg aagattgcag tgatcgacga
      421 ttaccagaac gcatttcgga cactcaagtg ttacccgaaa ctgcgcgacc atgaggttgt
      481 ggtatttacc gacacggaaa cggatatcgg taaattggct gataggttga aagacgcgga
      541 tgcggtggtg ctcacgcagc agcgatcgag ttttccgcgg gctctgatcg aaagattgcc
      601 aaacttgaaa ctgatcggcc aaaccggacg cgccgcgact cacgtggacc tcgatgcttg
      661 caccgagaag gggatcgtgg tttccgcggg cggcgccggc aactcaagcg caaccgcaga
      721 gctgacctgg ggactcatcc tgagcgcgct gcggaatctt ccatttgaag ttaaacggct
      781 taaagaaggt cactggcaat cgaccctcgg tatcggtgtc aatgggaaga cgctgggtat
      841 ttacgcttac ggcaaaatcg gcagtatcgt tgccgccgcc gggaaagcat tcggcgcacg
      901 cgtggtctgc tggggacgcg agggctcgac cggacgcgcc aaggccgccg ggtttgaagt
      961 agcgcagagc cgcgaagact tcttcgccga ggccgatatc ctgagcttgc atctgccgct
     1021 caacaaagac acccaaggga ttgtcacgcg cgacgatctc ggccggatga aaccgacggc
     1081 tctgatcgtc aacccaagcc gtgcggggtt gattgcgccg ggcgcgttgg ttgaagcgct
     1141 gaaagccggc cgccccggaa tggccgcggt ggatgtctac gaaaaagagc cggtgatcgg
     1201 cgccgatcat ccgcttctca gaatggagaa tgtcacctgc acgcctcatt taggttacgt
     1261 taccagggaa ggttacgagg actattacgc tgttgtggtc gatgatattc ttgccttcgc
     1321 cgcaggcaag ccgcaaaacg tgctcaatcc cccagcactg gcgaagaagt agaagatcga
     1381 ggaaattgca tctcaccatt gtaagcggga cggcatactg ctgtaagcgg gacggcatac
     1441 tgcaagcttt acgcaatcgc ctcggcacat cgtgccggtc ctgcacagct cgaaacacct
     1501 aacagtttgc gcgagaattt ttccataagc ttcttcatcc cgattgtaat cgaggcctca
     1561 taagagcgat ccggatctga gatcgctaaa gatccgccgt ggttcaggat cgttgcggtt
     1621 tatcgataag taatgcaagt agcgaagttg ttttgatggg ccgacccata ttgcttttgc
     1681 tcgttctcag gttaggcttt tggcatgcag atacaaatcg aattgaccga agaatcgcgg
     1741 gagttgctga tgcgccactt caaggagacc gcgtcgttgc acgaagtcct cgcccgcgct
     1801 cagcgaaatg aattggccgg agttaatgtc tactcgttcg aatgcgattt acagcaggca
     1861 acggaactgc tggaaatggc ccgggaccat tgtccgccgg cggtgaagga tatcgagcac
     1921 gccatttttg cggcaacggc gggttgagcc ttcgtttctg cggaactagc cagacgacgc
     1981 atgtcctctg aagcccttgt taagaatggt tgctcaatcg atcgagcacc cccacgtacg
     2041 acatcttagc tcctggactg aaatgatcgt gccactccaa ttatcgtgag caccacttca
     2101 cccggctcca tcagcatctg ttagctttcg gcatcggtgt aagctgtgcc gtctgcctca
     2161 accacgatcg caaagcggca gcggtatctc ggtgacactc tacccagtcg ccgctttgac
     2221 tagttaaatc cacatcggcg tgagctcaga aatttgatca agttccgtaa gttcaagcaa
     2281 gccagggata ggccccggtt cagttacatt cttcgatcca agctccggta ctgaatcgct
     2341 tcggcgatgt gactcgactg gatttcctcc gaacccgcga ggtctgcgat ggtgcggcca
     2401 actttgagca cacggctgta ggcacgggcg ctgaggccca gccggttaat ggcgttttcc
     2461 agaagctttt ctgcatcgtc tttgagctgg cagtgacgct tgatgtcacg ggcgcccatt
     2521 tgcgcattgc cgtggatgtg tcttttgagg aagcgctgca actgaatggc gcgtgcagca
     2581 ttgacccttt gccgaattga atcggaactt tcaccagcat ctttactggc gaggtcctga
     2641 tagcgaagcg ccggcacctc gacttgtata tcgatgcggt cgagcagggg cccggatacc
     2701 cgcgaacggt agcgttgaat ttgcaatggc gtgcagacgc actccttttg cggatcggtg
     2761 aaaaagccgc aggggcaggg attcatcgcg gcaaccagca taaagccggc ggggtaatta
     2821 atcgacccga tcgcgcgtga taccgtgatg ctgccatcct ccaggggttg acgcatgacc
     2881 tcgagcacgt tgcgcttgaa ctcaggcaat tcgtcaagga agagcacgcc gttgtgcgcc
     2941 agcgagactt cgcccggctt aggcacagtc ccgccgccga ccaaacccgc atcggagatg
     3001 gtatggtgcg gatagcgaaa agggcgcctc gcgataatcg gcgagccgtt gagtaggccc
     3061 atgacgctgt ggatcttggt ggtttcaagg gcctccgcaa gtgtgaggtc tggcaggatt
     3121 gtcggcagtt tgttcgccag catggtctta cctgagcccg gtggaccgac cataattacg
     3181 ttgtggcctc cggctgcggc aacttcgagt gcgcgcttga cgtgctgctg gcctttaacg
     3241 tcggtgaaat ccgagctgta ctgcgattgc tgccggaaca atgcttcaag gttgacatga
     3301 gtgcgctcaa tcgcgcgccg accgtttaga aattctacga cttcaggaag gtcactgaca
     3361 ccaagaactt cgacctcgct gacgaccgcg gcttcaggcg cgttgtgctt gggcaggatc
     3421 aatcctttga cgtgttctct ttttgccgcc atcgcgatgg gcaaagcgcc gcggacaggc
     3481 ttgatcgcgc catcgagcga tagctcgcct atgaggaaat aatcattaat cagattttta
     3541 tcgacagagc cttccgcggc gagaataccg atcgccatcg gcaggtcata agcggaacct
     3601 tctttcttga tatcggctgg cgcaagatta atcgtgatgc gcttggcggg gaaagtatag
     3661 ccgcagttct taagcgcggc gcgaatccga tctttgcttt ctttgaccgc gccctcaggc
     3721 agtcccaccg tagcgagttg gggcaatccc gaggccaggt cgatttcgac ttcgacgcgc
     3781 aaggcatcga tgccgtatag cgccgcgctg ttgatcttcg ctagcataaa ccggcgctac
     3841 gctagcaaag gcgttacagg ataacaactg gcagggtgct gaaaaaagcc tcctgcctgc
     3901 tagaatttat cttttttaaa agacttcagc gacgacggcc gcatgcgcaa agcgtcatct
     3961 ttcgtgacac gatcgaacac gtggacgcca cgattgtgga gtagggtggg tcgggagagc
     4021 gcccgtttag ctgcggcagc acagatcata catctcctga gcggcttgtc gctgactttt
     4081 tgaattgctt cgaagtgatg tcctttagag cattcgtatt cgtaaagcgg catacctgtg
     4141 gcgattaact aacacattga aaaaaccacc acaatgaagc gattagtttg tctgatgaga
     4201 ggtttcctgg tataagactt tatggttgct tcgagtcgat cgatcacgcc acactacctg
     4261 ctgcatgccc catttttagc aatcttgctc ggaggtgaga catgttagag caagaagttt
     4321 tgcccgtcgg attaactttc gatgatgtct tgttgattcc cgccgagtcc gcagttttgc
     4381 cgcgagacac cgatgtctca actcggttaa ctgaacgaat caccctgaac attccccttg
     4441 tcagtgccgc gatggatacc gtgacggagt cgaaaacagc cattgccatg gcgcaagaag
     4501 ggggcattgg cattattcat cggaatttgc ccgttggtat ccaagcggcc gaggtagaaa
     4561 gagtaaaaaa gttcgaaagc ggcatgattt ccgatccgat caccgtggcg ccggaccagc
     4621 agatctcgga agccaaggca atcatggaca aatatggcat ttcgggattg ccggtaaagc
     4681 gcaatgggcg gttagtcggt attttgacca atcgcgatct gcgcttcgaa aagaaactcg
     4741 atcgcacggt ttccgaggtg atgactaaag ataaattaat aaccgtgaaa gagggggtcg
     4801 atctcgacca agcgaaagag cttttgcacc ggcatcgtat cgaaaagctt ctagtggttg
     4861 atgatgactt caatctcaaa ggattaatta ctgttaaaga cattgagaag cggacccagt
     4921 atccaaatgc ttgtaaagac acgcgaggcc ggttgcgggt tggagctgcg gtcggagtgg
     4981 ggccggattg ggaagagcga gtcgaggctc tggtgcgcgc gggggcggat ctcatcgcca
     5041 tcgatacggc gcacgggcat tcaagaaacg tgctggatac catccggcaa atacggggac
     5101 gctatccgga tcttgatcta tccggcggca acgtggctac agccgaagga acaacggctt
     5161 tgattcaggc gggagtcaat gtagtcaaag ttggcgtcgg ccccggatcg atttgcacga
     5221 cccgggtggt ttccggagtg ggagttcccc aggtgacggc catcggcagt tgcgccaagg
     5281 cagcggcgcg ccataatgtg ccgattgtcg ccgatggcgg catcaaattt tccggcgata
     5341 tcaccaaagc tctcgccgct ggagccgata gtgttatgat cggcagcctc tttgcgggaa
     5401 ccgaagaaag tcccggcgag acgattctct accagggacg aacctacaaa gtttatcgtg
     5461 gcatgggatc gttaggcgca atgaagcagg gcagcaaaga ccgctacagc cagagtgatg
     5521 ttgaagagac catgaagctc gtccctgaag ggatcgaagg acaggtgccg tacaaaggtt
     5581 cgttatcaat caatattcac cagctggttg gaggcctgcg cgctggtatg gggtatctag
     5641 gctgccggga tgtcgccacc cttcgatcta aagccagatt catgcaaatc accgccgcgg
     5701 gattgagaga agggcatgtt catgatgttt tcatcacgaa agaagcccct aactacagcg
     5761 tcgaatgatc ctcgttcttg atttcggttc tcaatacacg caactcatcg cgcgccgtat
     5821 tcgcgaatcc aatgtgtact gtgagatcca tcctttcagt gtcggcccgg ataaaatctc
     5881 ggaactaaat cccgagggga ttattctttc ggggggaccg gctagcgtct accaggacaa
     5941 cgcacctaaa gttaaccctg agatatttga cctacccgtg ccgttcctag gcatatgcta
     6001 cggcatgggc gtgatgaact tggcttcggg cggcgccgtc gcccgggcgg atcatcgcga
     6061 gtacggtccc gccgatctga tgatcgatag cgacgccgat ctgttttacg gattcaaaaa
     6121 gaacgatccc gtgcgggtgt ggatgagtca tggcgataaa atgaccgctg tgcctacggg
     6181 ctggagcagg atcgcgcata gcggcaattc gccgatcgcc gcgttcgccg atccgacccg
     6241 tcggtttttt ggcgtgcagt ttcatcccga agtcgcgcac actccgcgcg gcaaggaaat
     6301 tctcgacaac tttatttttc gtatctgcgg ctgcaagccc gactggacaa tggaacattt
     6361 tatcgagact tccgtgcgga agattcgcga tcaggtgggt agtggacgcg ttctgtgcgg
     6421 ccttagcggc ggtgtcgatt catccgtggc cgccaccttg attcatcggg cgatccaaga
     6481 gcggctgata tgcgttttcg tcaataacgg tttgttgcgc gagggagaag ccgagcgcgt
     6541 gtgccagtta ttcagtgaac ggtttggcag tagctttcgc tacgtcgatg ccagtgaggc
     6601 ttttctgtcc gacttgagcg gcatcgaaga tccggagata aagcgtaaga aagtcggcta
     6661 caaattcatc gaagtttttg aaaacgaggc cgaaaaatta ggcgagatcg attttctcgc
     6721 ccagggaacg ctttatccgg atgtgatcga atctgtttcg tttgtcggtc catccgtcac
     6781 catcaaaagc catcacaacg tcgggggttt gccatcggtt atgaagctga agctcatcga
     6841 accgctgcgc gagctattta aggatgaagt taggaccttg ggtaaagaac tcggtttgcc
     6901 tcacgaaatg attcatcggc agccgtttcc cggacccggg ctggcgatcc gtattctcgg
     6961 gccagtgacc gaggagcgtc taaagatcct ccgcgcggcc gacacgatcg tctggcatga
     7021 aatgcgtgcc gccggtctgt atgaaaaggt ctggcagtct tttgcggttt tgctgccgat
     7081 aaaaactgtc ggtgtcatgg gagacgagcg cagctatgag aacgtcatcg ccttacgagt
     7141 agtagagagt ctcgacggca tgactgccaa ctgggttcct ttacccgccg atttgctatc
     7201 aagaatctcc aataggatca tcaatgaagt tcgaggcgtg aaccgcgtcg tctatgatat
     7261 ctcttcaaaa cctcccgcta ccatcg
//
LOCUS       DASUTG010000202         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_329344, whole genome shotgun sequence.
ACCESSION   DASUTG010000202 DASUTG010000000
VERSION     DASUTG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329344"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..635
                     /locus_tag="VF089_04515"
     CDS             357..635
                     /locus_tag="VF089_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173255.1"
                     /translation="MMRAFDTVTTDELVALPPTIFGVIRWSRYIGRPMGDLCSGFNIR
                     VEEHTATQFRAVGGGKYEPIPGTGIWKDVVDALSCREVADAKSKSIAS"
     gene            857..1042
                     /locus_tag="VF089_04520"
     CDS             857..1042
                     /locus_tag="VF089_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEX6173256.1"
                     /translation="MELKDLAQRIREACLEAVLQAYEDAGMQSLCAEGRWEAAVDALR
                     TVDLAPLLREFQQPPAD"
     gene            1287..2150
                     /locus_tag="VF089_04525"
     CDS             1287..2150
                     /locus_tag="VF089_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6173257.1"
                     /translation="MAEYYALQAAFCFSVAHIFVRRGLVHSNALTGSLISLGTSATGF
                     WILAVLFAPLALLKNSAMGYFAAAGLFAPAIGQTLGYMGMERIGVARASAIVNTSPIF
                     SSMFAVLLLGELWLAQNIVGTCLVIAGIAVLSSTQGSVTADWRRKDLVFPLLGAVAFG
                     ISTLLRKRGLVEVPEPLLAAAVTVGSAFFFFLLIVGFRGGQSALKFERRGTAWLFAAA
                     LVNMAAILSFFSALNLGKVVRVEPLVACNPLLTILWSVLFFRGLEQLTARDIVCALII
                     ITGTVLVVTAK"
     gene            2154..>2959
                     /locus_tag="VF089_04530"
     CDS             2154..>2959
                     /locus_tag="VF089_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6173258.1"
                     /translation="MDIYFSFQAALFFSIAHILIRRGLVESNAMTGSFISLSMTAAIL
                     WLMFPFFASFSALWTPVSLIFVAAGIFAPGIGRTMSYIGIEKIGVARSVPVANSSPIF
                     ASIFAVIFLAEVWVPQNIVGTLLVISGVISLSISRPAQGPWRKLDVIYPLIGAMAFGA
                     SANLRKAGLDYVPVPVLAAAITAGSAALFSFILLQVRGGKAALKLTRRSASWLFTAAL
                     FNTAATLSVFYALSYGKVVIVEPLVSSNPVLTLLFTAIFLRDLEAINLRV"
BASE COUNT          548 a          813 c          867 g          731 t
ORIGIN      
        1 gggcgaggac gtggagcgag aaatcaaggg cctgttcaat atggagccag ccttaattga
       61 taagctaaag gatattctaa agtgaaagcc gtgatgctgg aaatacctaa ctaagccgct
      121 aaagacagag cttcagtcgc gcctgagttg ttattcgagc gtcaagaagc tgcttggcga
      181 cgacaaaaca agtgaacgaa gggtttgcca agattggcta gtcaagttgc aacgacacat
      241 gcagttgccg agaagtgaag gtctgagtca ggcatcggga gcaatgttaa ggtaagatcc
      301 tactactgcc gtgttgaagg aaatgtgaaa tattcggggc ggcatgagga gaggaaatga
      361 tgcgagcgtt tgacacagtg acgaccgacg aactcgtggc actaccgccg acgatctttg
      421 gcgtcatccg ttggagcaga tatatcggca ggcccatggg tgatctgtgc agcggtttca
      481 acattcgtgt ggaagaacat acggcaaccc agttccgcgc tgtcggcggt ggaaagtacg
      541 agccgattcc cggcaccggc atctggaaag atgtggtgga tgctctttct tgccgggagg
      601 tagcggatgc aaagagtaaa tcgattgcat cttagctagt caaatcagga ccgcttgatt
      661 gagccgctct cttaagtact tgtcatgtgc tcgaatccct agatagtcat cgtcggaaac
      721 atttcggacg atcttctcgt tgttaccttg gaggatgccg gccagagcgc atcgaggttt
      781 ctctgagctg caattgagat ggtgggaagg atcgagacat tctttttgtc gattactccg
      841 gtctctggga gctattatgg agttgaaaga tctcgcccaa cgcattcgag aagcatgcct
      901 ggaggcggtc cttcaagcat acgaggatgc gggtatgcaa agcctctgtg cggaaggacg
      961 ttgggaagca gccgtcgacg cgctgaggac ggttgacctt gcgccgctgt tgcgtgagtt
     1021 tcaacagcct ccggcagatt gaccggccta agcagcgcat gcagatgatt gtccagacgg
     1081 cactaacact gttgcaagtg acgcttggga ttgggtatga ctgtgtgtca ttacaaggtt
     1141 gaccctcttc taagcacccg gccgcagccg cgcctggtca gcactgagcg gtttggaatt
     1201 aacacgagga atcatcgctc gcgcgcccgc ttgaatctgc cggcagcggg gctcactgaa
     1261 gcgatccatt ttcttgcccc gacaccatgg ctgaatacta cgcgctgcag gccgcgttct
     1321 gcttttcggt cgctcatatt ttcgtgcgcc gcggcttggt tcattccaat gctcttaccg
     1381 gctcgctgat ctccctcggc acgagcgcga cgggattctg gatcctggca gttctatttg
     1441 ctccgcttgc gcttctgaaa aattccgcca tgggctattt cgccgcagcc ggcctgtttg
     1501 cgccggcgat cggccagact ttgggctata tgggcatgga gcgcatcggc gtagcgcgtg
     1561 cgtcggccat cgtcaacact tcaccgatct tctcgtccat gtttgcagtg ttgcttctcg
     1621 gcgagctatg gctggcgcag aacattgtcg gtacgtgcct cgtcatcgcg ggtatcgccg
     1681 tactctcctc cacccaagga tcggttaccg cggactggcg cagaaaggac ctggttttcc
     1741 cgctcctcgg ggcggttgcg ttcggcatct ccaccctgct ccgaaagcgc ggcctggtcg
     1801 aggtgcccga accgctgctc gccgcggcgg tgacagtcgg ctcggctttt ttctttttcc
     1861 tgctgattgt cggctttcgc ggcgggcaaa gcgcgctcaa gttcgaacgg cgcggcaccg
     1921 catggctctt cgccgcggcc ctggtgaata tggccgcgat tctctcgttt ttctccgcgc
     1981 tcaatcttgg aaaggtagtt cgggtggaac cgctggtcgc gtgcaatccg ctgttgacga
     2041 tcctctggag cgttttgttc tttcgcggct tggagcaact gacagcgcgc gatattgtct
     2101 gcgccctcat catcatcacc ggcaccgtgc tggtggtcac cgcaaaataa ccaatggata
     2161 tttacttctc gtttcaggcg gcgctcttct tctccatcgc gcatatcttg atccggcgcg
     2221 gcttggtcga atccaacgcg atgaccggct cgttcatctc gttgtcaatg actgcggcga
     2281 tactgtggct catgtttccc ttttttgcgt ccttctcggc tctgtggacg ccggtgtcgc
     2341 tgatcttcgt cgccgccggt atctttgctc ccggcatcgg ccgtaccatg agctacatcg
     2401 gcatcgagaa gatcggcgtc gcgcgctcgg tgcccgtcgc caactcatcg ccgatctttg
     2461 cctcgatttt cgcggtgatt tttctcgccg aagtttgggt gccgcagaac attgtcggaa
     2521 cgctgctggt gataagcggc gtcatttcgc tgtcgatctc gaggcccgcg caagggcctt
     2581 ggcgtaagct ggatgtaatc tatccattga tcggcgccat ggctttcggc gcctcggcga
     2641 atttgcgcaa ggcaggcctg gactacgttc cggtcccggt gctcgccgcc gcgatcaccg
     2701 cggggtcggc ggcgctcttc tcgtttatcc tgctgcaggt ccgcggcggc aaagcggcgt
     2761 tgaagctcac gcgccgcagc gcgagctggc ttttcaccgc cgcgctcttc aataccgcag
     2821 cgacgctctc cgtgttttac gctctcagtt acggcaaagt cgttatcgtc gagcctctgg
     2881 tgagttccaa tccggtgctg actctgctgt ttacggccat ctttcttagg gatctggaag
     2941 cgatcaattt acgggtgat
//
LOCUS       DASUTG010000203         8977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1959903, whole genome shotgun sequence.
ACCESSION   DASUTG010000203 DASUTG010000000
VERSION     DASUTG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959903"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="VF089_04535"
     CDS             complement(<1..1710)
                     /locus_tag="VF089_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX6173259.1"
                     /translation="MPKPDLPICEIEQRLLKSLTTQRRLIVTAPTGSGKSTQVPQMLL
                     DSGLLRDGQVTVLQPRRLPTRMLAAWVARTRGVKLGGEVGYQMRLDNVTSAATRICYL
                     TEGVLLRRMLADPDLTGVSAIVFDEFHERHLFGDITLARARQIQATTRPDLFILVMSA
                     TLDAAAVQNYLHPCALLSSEGRAYPVTVEYLAKPAGGISVWELAVRELQGLLHTQPQG
                     DALIFMPGAYEIMRTVRAARDALGPEFLIFPLHGELPPADQDAAMARYNRRKIIVATN
                     VAETSLTIDGVRLVIDSGLARSPRYDPYRGVNTLLIEKISRAAADQRAGRAGRTAPGH
                     CLRLWTAHEQNARPAQELPEVKRLDLSEVILTLKAGGLNDLTRFQWLEAPDPRALKRA
                     ETLLADLGAIEDIAGAITVLGQRMIAFPIHPRYARMLLGGREYDCVRPVALIAALTQG
                     RDLLMRRQTDPTEDRRGDLFGDASESDYFVLMRAWKYARRNGYDDERCRRMGIHAQAA
                     RQVESLFEQFLSIAAAEGFDIRDKPMAPEAVQRCVLLGFCDHVAKRLSPGSQRCEMTH
                     GRRG"
     gene            complement(1795..3255)
                     /locus_tag="VF089_04540"
     CDS             complement(1795..3255)
                     /locus_tag="VF089_04540"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6173260.1"
                     /translation="MTRRGEEYVRGLRDGRTVLLNGERVSDVTSHPAFAAGIRSIAKL
                     YDLAADPANRELMTYPSPRDGRPINKSWLVPRSRDDLAARRYAIKFWADASYGLLGRS
                     PDHVASFFAGFAGSPEFYARGGREFADNLSRFAAKAADEDLYVSYVIVHPTVDRAKPA
                     HQQAEPYLYAGAAAERDNGIVIRGAQMLGTGSVMSDYVLLTVILPLKPGDEDYAISCV
                     VPNSASGLKLYPRRPYALGSNSVFDYPFSSRLDETDSLVVFDDVLVPWEDVFIYKNIE
                     LTAGQFSVTAAHVLGNTQSHIRSWAKLQFLVGLVKRCMDLSGRSANIEILAQLGDLAT
                     RVSLVEGMILGAEAAAEPDQFGVMRPKDSLLYASQVFQQAMYPALLNQIRGMMGGSLI
                     QLPATVAELQAASSALDIDRYVRWPKSSGAERVKLLKLLWDAVGSEFGSRHLQYEMFY
                     AGEPAAIQGREFRTFDWAAAEALVDRCLATYDESTV"
     gene            complement(3504..3944)
                     /locus_tag="VF089_04545"
     CDS             complement(3504..3944)
                     /locus_tag="VF089_04545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6173261.1"
                     /translation="MADANSIATLSRDLIEYGLNWRWTPVRVAASIRAANVNVLVASM
                     HEEIAGFAIMRYGDDDAHLDLLAVAPPYRRSGIGRQLMEWLEECAIVAGIFNVDLEVR
                     AGNEEAQIFYSRMGYRTLLQLPGYYQGVEAALRMGRDLSRRSLD"
     gene            4258..5253
                     /locus_tag="VF089_04550"
     CDS             4258..5253
                     /locus_tag="VF089_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173262.1"
                     /translation="MIKMTMTLRYGAWFVVLVLMTSLNRAEAQQTPKLTPVNYGTITI
                     SALHWPYLIAEQEGMLQKEGIEIKRVMGGTTTATSQALVAGSTDFAQMNLVQLLSANS
                     AGADLIAVAGDTLVPIYALVVHPSIKSYADLKGKRLAVAGPTDPLNYIMARMLAANGL
                     TPADYEMLGLGGTPTRLAAVEKGGVAGALVNQPSDFKAIASGLRFLGYSSDYVDNFQY
                     TVTGTRRDWAQANRPLTVRFLRAFARACEFFYEPKNKEAVLRALIEKAKAERDDAEKT
                     YNLFVKTKKSIPQDGAIDIKGARIVAESWKEFGLQKPPPPVDGVIDMSYLQEARK"
     gene            5267..6424
                     /locus_tag="VF089_04555"
     CDS             5267..6424
                     /locus_tag="VF089_04555"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate cyclase domain-containing protein"
                     /protein_id="HEX6173263.1"
                     /translation="MKNDELSYNLGDTVDRLISVQYFMSGGGSALTRKPVTGVLYEAA
                     REKAGAPLCHLAAKALADNVQRGDTVLITTGLIAPPYMIAEGDGPIAAATLARAIDVG
                     LDATPIIVCEGVVVKAMEALCQAAGLMPSAPELAVGTPRKVTVLPFPLDDDLGRREAI
                     RLLDQFKPKAVVAIEKLSKNAKGIYHNGFGIDVSSVQAKVDYLFEFARERGIPTIGVG
                     DGGNEIGTACIADAVRKVVPTGTKCGCPCESGIAAATDADYCVFGSKSNYGGFAMEAC
                     LAAGLERPEIFHSEVVARRVHEAGATMGLVDPFTGLAFGWDDFPVEKNVNELVTNLIR
                     YMLAVRVVPRWYKDSIMGPSQKWIHERSFAEDLVSRWAKSIIDSEKEFFPK"
     gene            6429..7859
                     /locus_tag="VF089_04560"
     CDS             6429..7859
                     /locus_tag="VF089_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173264.1"
                     /translation="MAKNPAELVFKNGLIVTPSGVVEGGLAVVDGKIATIGSDASLPA
                     ADRQIDLSGKYLLPGVIDPECHLGGHRDLAADFKSETRAAAASGVTTWGLQLVSLAIT
                     GGADKVKGPADIPNFAKSMPIFYEASRTAAIDFFLTPIVSTDEQVKEIPYLANEHGIS
                     SFKLHLQMQGPWKSSWPAYFFDDGSIYSVFQEVAKLGAPAIALLHCENWEIAQVLQKQ
                     LIDAGRTDMGAWDDRSPAFTEVGHARTYLYYARIAGCPIYVVHCTTTDTVAEIERAKA
                     EGLTVYSEIGTHYLVLHKDAWKINVPLRDRSTHEDLWNALASGAIDCIGSDHVAHTRT
                     RESMETGNVFTTISGFPSRVEGMLPMMLSQGVNKGRISIERLAQVASENPARIFGLYP
                     KKGAILPGADADLVVVDLKRRATITKELLHTVTPWSIYEGWDVTGWPVMTVVRGNIVM
                     EWPEGAPRAIVSETPTGQYLRRQLGT"
     gene            complement(7909..8118)
                     /locus_tag="VF089_04565"
     CDS             complement(7909..8118)
                     /locus_tag="VF089_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173265.1"
                     /translation="MIQDLLPEDYQIQSRIKDFGLSVFARIAGIRIFSLRLIRSLGRL
                     VLMPNTARYAFTGWPAGATKSQRAC"
     gene            8243..>8977
                     /locus_tag="VF089_04570"
     CDS             8243..>8977
                     /locus_tag="VF089_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173266.1"
                     /translation="MPPVISISNLTKTYASGYQALKGIDLEIRKGEIFALLGPNGAGK
                     TTLISIVCGIVTPTQGIVIVDGHDIVREYRAARSKIGLVPQELTTDAFESVWATVSFS
                     RGLFGHAPAPAFIEKVLRDLSLWEKRKNKIMTLSGGMKRRVMIAKALSHEPEILFLDE
                     PTAGVDVELRRDMWALVRTLRESGVTIILTTHYIDEAEEMADRIGVINKGGLVVVEEK
                     VELMKKLGKKQLTLSLLDPLPSLPLEL"
BASE COUNT         1970 a         2594 c         2600 g         1813 t
ORIGIN      
        1 accacgccgg ccgtgcgtca tttcgcaccg ctgcgagccg ggactaagac gtttggcgac
       61 atggtcacaa aatccaagca gcacgcaacg ctgcaccgcc tccggcgcca tcggcttgtc
      121 gcggatgtcg aaaccttcgg ccgcggcaat gctcaagaac tgctcgaata gcgactcgac
      181 ctggcgcgcc gcttgagcgt gaatgcccat gcgacggcaa cgctcgtcgt catagccgtt
      241 gcgccgggca tacttccagg cgcgcatcag cacgaagtag tccgattcgc tcgcgtcgcc
      301 gaacagatcg ccacgtcggt cctctgtcgg gtcggtttgg cggcgcatga gcaagtcacg
      361 cccctgcgtc aaggcggcga tcaatgccac cggtcgcacg caatcgtatt cgcggccacc
      421 cagcagcatg cgggcataac ggggatgaat cggaaacgcg atcatgcgct gcccaagtac
      481 ggtgatcgca ccggcgatgt cctcgatggc tcccaaatcg gcgagcaacg tctcggcccg
      541 tttaagcgcg cgcggatcgg gggcttccaa ccactgaaat ctcgtcaagt cgtttagccc
      601 gccggctttg agagtgagga tcacctccga gaggtccaaa cgttttactt ccgggagttc
      661 ctgggccggg cgcgcgttct gttcatgcgc ggtccacaga cgtaaacagt gcccgggcgc
      721 agtgcgcccc gcgcgacccg cccgttggtc ggctgcggca cggctgattt tttcgatcag
      781 caacgtgttg acgccgcgat acgggtcata gcggggactc cgagccaaac cgctatcgat
      841 caccagccgc acaccgtcaa tcgtgagcga agtttccgca acattcgtgg cgacgatgat
      901 tttacgccga ttatagcggg ccatcgcggc gtcctggtcg gccggcggca attcaccgtg
      961 cagcgggaat atcaggaact ctgggcctaa agcatcgcgc gcggctctta cagtgcgcat
     1021 gatctcatag gcgcccggca taaaaatgag agcatcgcct tgcggttgcg tgtgtaacag
     1081 gccctggagc tctctgaccg cgagttccca gacggatatc ccacccgcag gtttcgccag
     1141 atactccacg gttaccggat aagcgcggcc ttcggacgac aacaacgcgc aagggtggag
     1201 atagttctgc actgccgcgg catccaatgt cgccgacatc acgaggataa agagatcagg
     1261 ccgggtcgtt gcctgaatct gccgggcgcg ggctaacgtg atgtcgccga agaggtggcg
     1321 ttcgtgaaat tcatcaaaca cgattgcgct gacgccggtt aaatcgggat cagccaacat
     1381 ccggcgcagc aggacacctt ccgtcaggta acaaatacgg gtcgcggcag aggtcacgtt
     1441 atccaagcgc atctggtaac ccacttcgcc gccgagttta acgccgcgag ttcgagcgac
     1501 ccaggcggcg agcatacggg tgggcaagcg gcgcggctgt aacaccgtca cttgaccgtc
     1561 gcgcaataag ccactgtcga gcagcatctg cggtacttgg gttgacttgc ctgagccggt
     1621 gggcgcggtg acaatcaagc gcctctgtgt cgtcagtgat ttgagcaacc gctgctcgat
     1681 ttcacaaatc ggtagatccg gcttcggcat agatgaagaa tatggagggt atcaacggct
     1741 gttcggaagc gaaggatcct ggcgctgtta tgaagagaaa tttcgctgcc gccgctacac
     1801 agtgctctca tcgtaggtcg ccaaacaacg atcgaccaat gcctccgcag ccgcccaatc
     1861 gaaggtgcga aactcgcgtc cctggatagc cgcgggctcg ccggcgtaaa acatctcgta
     1921 ctgcaaatgg cgcgaaccaa actccgaacc cacggcgtcc cacaagagct tcagcagctt
     1981 cacccgctcc gctccgctgg atttcggcca gcgcacgtaa cgatcgatgt cgagcgccga
     2041 ggacgctgcc tgcagctcag ccactgtcgc cggcaactgg atcaggctgc cgcccatcat
     2101 tccgcgaatc tggttgagca acgccggata cattgcctgc tggaatactt ggctggcata
     2161 aagcagggag tctttggggc gcatgacgcc aaactggtcc ggctccgccg ctgcttcggc
     2221 gccaaggatc atgccttcca cgagcgagac acgagtggcg aggtcgccga gttgcgcgag
     2281 gatctcgata ttggccgagc ggccgctcag atccatgcag cgtttgacca gccccaccag
     2341 gaactgcagc tttgcccacg agcggatgtg tgattgcgtg tttcctagca cgtgcgccgc
     2401 ggtaacactg aactgacccg ctgtaagctc gatgttctta tagatgaaca cgtcctccca
     2461 tggcaccaga acatcatcga agacgaccag cgaatcggtt tcatcgagac ggctcgagaa
     2521 cgggtaatcg aatacgctgt tcgatccgag cgcatacggc cggcgcgggt agagcttcag
     2581 accggaagcg ctattcggaa ccacacagga aattgcgtaa tcttcgtcgc cgggcttgag
     2641 cggcaaaatc accgtgagca gcacataatc ggacatcacc gagcctgtgc cgagcatctg
     2701 tgcaccgcga atgacaatgc cgttgtcgcg ctcggcggcg gcgccggcgt ataggtatgg
     2761 ctcggcctgc tggtgtgccg gtttggcgcg gtcgacagtg ggatggacga tgacgtagct
     2821 aacgtagaga tcctcgtcag cggctttggc tgcgaaacgc gagagattgt cagcgaactc
     2881 tcggccgccc cgggcgtaga actcaggcga gcccgcaaat cccgcgaaaa acgatgccac
     2941 atggtcaggc gagcgcccga gcaagccata gcttgcatcc gcccagaatt taatcgcgta
     3001 ccgccgcgcc gccaggtcat cacggctgcg cggcaccagc catgacttgt tgataggacg
     3061 gccgtcacgc ggcgacggat aggtcatcag ctcgcgatta gcgggatcgg cggcaagatc
     3121 gtagagcttg gcgatgctcc gaattccggc ggcaaaggcc ggatggctcg tcacatccga
     3181 gacacgctcg ccgtttagca gtaccgtgcg accatcgcgc aagccgcgca catactcctc
     3241 accgcggcga gtcacgtctg actcccttgc cgcgcttggt tgctaaatcg cacgtttttc
     3301 tgaaaccgtc agtgtttatc gacttcctgg ccatgatcag attatcctct agtattgatg
     3361 tcggactgtt gcaaactcgg cagcggtgcg tgaagagaga ctgctcatgc tcgctgccgt
     3421 catcgcttgc gcgaaaaatg gcgaatgcaa cgatgagtat cagtcttttc agcaggcttc
     3481 tatgattgct tcctgtgagt tctctagtcg agagaccggc gggacaaatc tcggcccata
     3541 cgaagcgccg cctcgacacc ttgatagtag ccaggaagct gaagcagtgt gcggtaaccc
     3601 attcgactat aaaagatttg cgcttcttca ttgcccgcgc gcacttcgag atcaacgttg
     3661 aatatgcccg ctacgatggc gcattcttcc agccactcca ttagttggcg cccgatgccg
     3721 cttcgcctat agggcggagc cacagcgaga agatccaaat gtgcgtcgtc atcgccatag
     3781 cgcatgatgg caaagccggc aatctcttcg tgcatagatg caaccagtac gttgacgttg
     3841 gcagcgcgaa tactcgctgc cacccgcaca ggtgtccagc gccagttgag accgtactcg
     3901 atcaaatcgc gtgagagagt ggcaatgctg ttagcatctg ccaaacgcgc caaccgcaac
     3961 gatagctcag aatttatcat cagcctgcac gacctacgga ccagattttc ccgcaaagta
     4021 acggagcaat caagttcttg ttctatgatg tcgtcgggct cactgtgatc cacatgtcaa
     4081 atgcaaacca tagccgagta atgtctcgtc ggcggcacca ttcacgggct gacattagtt
     4141 gggtcagctc gtctaacttt gattgacagg cgctcgaggt catagtacga aacggccagc
     4201 tgaatgagaa cgcctggcgg attttgaatc gagaacttac tctctaaagg atgcacgatg
     4261 ataaagatga caatgacact caggtatggt gcctggttcg ttgtactcgt attgatgacc
     4321 tcactgaacc gcgctgaagc gcagcaaact ccaaaactga cgccggtcaa ctacgggacc
     4381 atcactatat ctgcattgca ttggccctat ctgattgctg agcaggaagg catgcttcag
     4441 aaggagggca ttgagatcaa gcgggtgatg ggcggcacca ctaccgctac ttcccaggcc
     4501 ctggtggccg gctctacgga tttcgcgcag atgaatttgg tgcagctgct ctccgctaac
     4561 tcggcaggag ccgacctcat cgccgtcgca ggcgataccc tggtgcccat ctatgcgctg
     4621 gtcgtgcatc cttcgatcaa aagctatgcc gatctgaagg gaaaacgatt agcagtcgca
     4681 ggcccgaccg atcctctgaa ctacattatg gcgcggatgc tggcggccaa cggtctcacg
     4741 cctgccgact atgaaatgct cgggctgggt ggaactccaa ctcgcctggc cgcggtggaa
     4801 aaaggcggcg tcgccggggc gctggtcaat cagccctcgg atttcaaggc catcgcttcg
     4861 ggactgcggt ttctcggcta ctcatcggat tacgtcgaca actttcaata tacggttacc
     4921 ggaacgcgcc gcgactgggc gcaagcaaat aggcctttga cggtgcgctt tcttcgcgct
     4981 ttcgcgcgtg catgcgagtt cttctatgaa ccgaaaaata aggaggccgt gttgcgggcg
     5041 ctaatagaaa aggcgaaagc cgagcgtgat gatgccgaga agacctataa tctttttgtt
     5101 aaaaccaaaa aatcgattcc gcaggatggc gccatcgata tcaaaggcgc acgcatcgtc
     5161 gccgaaagct ggaaagaatt cggtttgcaa aagccgcccc cgccggtcga tggcgtaatc
     5221 gatatgagct atctgcagga agcaaggaag taggacacgt tcaacagtga aaaacgatga
     5281 gctctcatac aacttaggcg acaccgtcga tcgcctgatc tcggttcaat atttcatgtc
     5341 gggtggtggc tccgccctga cccgaaagcc ggtaaccggc gtgctctacg aagccgcccg
     5401 cgaaaaggcc ggcgcgccgc tttgtcatct tgcggccaaa gcgcttgcgg acaacgtcca
     5461 gcgcggcgat accgtgctga tcaccaccgg cctgattgcg ccgccataca tgatcgccga
     5521 aggcgacggc ccgatcgccg ccgcgacgct tgcgagggcc atcgacgtcg gtcttgacgc
     5581 gaccccgatc atcgtttgcg aaggagtcgt ggtgaaagcc atggaggctc tttgccaggc
     5641 cgccggcctg atgccgagcg cgcccgagct ggccgtcgga acgccgagaa aagtcaccgt
     5701 tctaccgttt ccgctcgacg acgatttggg gcggcgcgaa gcgattcggc ttttggatca
     5761 gttcaaaccg aaagcggtcg tggccataga aaagttatcg aagaacgcca aaggcattta
     5821 tcacaatggc ttcggcatcg acgtctcgtc ggtgcaagca aaagtagatt acctttttga
     5881 gttcgcgcgc gagcgcggca tacccactat cggcgtaggc gatggtggca acgagatcgg
     5941 caccgcctgc atcgccgatg ccgtccgcaa agtcgtgccc accggcacca agtgcggctg
     6001 tccatgcgag tccggcatcg ccgccgcgac cgacgccgac tactgcgtgt tcggctccaa
     6061 gtcgaactac ggcggctttg ccatggaggc atgtttggct gcgggacttg agcggccgga
     6121 aatctttcac agcgaagttg tcgcgcggcg cgtacatgag gcgggcgcaa ccatgggtct
     6181 ggtggatccg ttcaccgggc tcgcctttgg ttgggacgat tttccggtgg aaaaaaacgt
     6241 caatgagctg gtgacgaatc tgattcgcta catgctagcc gtgcgcgtgg tgccgcgctg
     6301 gtataaagac tccatcatgg gaccgagcca aaagtggatt catgaacgct cgtttgccga
     6361 agacctggtc tcgcgatggg cgaaatcgat catcgattcg gaaaaagaat tcttcccgaa
     6421 gtaattttat ggctaaaaat cctgctgagc tggttttcaa gaacggcttg atcgtcactc
     6481 catccggagt cgtcgaaggc ggcttggctg tcgtcgacgg caagatcgcc accatcggta
     6541 gcgatgcatc tttgcctgct gccgatcggc agatcgattt aagtggaaaa tatcttcttc
     6601 cgggagtgat cgatcccgag tgccacctcg gcggccatcg cgatctagcc gccgatttta
     6661 agagtgagac ccgagcggca gcggctagcg gtgtgacgac ctggggtttg cagctcgtca
     6721 gcctggcaat caccggcggc gccgataaag tgaagggacc ggcggatatt ccaaacttcg
     6781 ccaaatcgat gccgatattc tacgaggcgt cgagaaccgc ggccatcgat ttttttctca
     6841 cgccgatcgt cagcaccgac gagcaggtca aagagattcc ctatctagct aatgaacacg
     6901 gcatcagctc cttcaagctc cacttgcaga tgcaggggcc gtggaagtcc tcatggccag
     6961 cttacttttt tgacgatggc agcatttact cggtctttca ggaggtcgcc aagctcggcg
     7021 cgccagcgat cgcgctgctt cactgcgaga attgggagat cgcccaggtg ctgcagaagc
     7081 agctcatcga cgccgggcgc accgacatgg gcgcctggga tgatcgctcg ccggctttca
     7141 cagaggtcgg tcacgcccgg acctaccttt attatgcccg gatcgccggc tgtccaattt
     7201 acgtcgtgca ctgtacgacg accgataccg ttgcggaaat cgagagagcc aaagccgaag
     7261 gcttgacggt ctacagcgaa attggcaccc actatctcgt gctgcacaag gacgcgtgga
     7321 agatcaacgt gcctctgcgc gatcgctcta cacacgaaga cttatggaat gcgctcgcgt
     7381 ccggggcaat cgattgcatc ggcagcgatc acgtcgcgca caccagaacc cgcgagtcca
     7441 tggagacagg aaacgtcttt accactatca gcggttttcc gtcacgggta gaaggcatgc
     7501 tgccgatgat gctcagccag ggcgtgaata aaggccggat ctccatcgag cgcctggcgc
     7561 aagtcgccag tgaaaatcca gcgcgcatct tcggcctcta tccgaaaaaa ggcgcgatcc
     7621 ttccgggcgc cgatgcggat ctggtggtcg tcgatttgaa gcggcgtgca acgatcacca
     7681 aagaacttct tcacacggtg acgccgtggt ccatctacga aggctgggat gtcaccggtt
     7741 ggccggtcat gactgtggtc cgaggcaata tagtgatgga atggcctgag ggcgctcctc
     7801 gtgccatagt cagcgaaact cctacgggcc aatatctcag aaggcaactc ggcacctaat
     7861 tctggggctc ttggagagag ccgtccgcac ggctgagatc attccagttt agcaggctcg
     7921 ttggcttttc gtagcgccgg caggccagcc ggtaaaagcg tatctagcgg tattcggcat
     7981 caaaactaat ctccccaaag aacgaatcaa tctcaatgag aaaattcgaa ttccggcaat
     8041 ccgcgcgaaa actgatagac cgaagtcttt gatgcggctt tgaatctggt aatcttcagg
     8101 cagcagatct tgaatcatta gaaatcgcta tcactccagg gcatgcgctg tcattctggt
     8161 cgtgattact cgagcaaagc ctgcttgatt gacgtccgtg tatcgacggc gcatcaccat
     8221 gcccgtcatc taagatcatc ccatgccgcc agtcatatcc atttcgaact tgacgaagac
     8281 ctacgcttcc ggctaccagg cgctgaaggg tatcgacctc gagattcgca agggtgaaat
     8341 ctttgccctg ttgggtccca acggcgccgg caaaaccacg ttgatcagca tcgtctgcgg
     8401 tatcgtcacc ccgacacaag gtatcgtgat cgttgacggc cacgacatcg tccgcgaata
     8461 ccgtgcggcg cgatcgaaga tcgggctggt gccgcaggaa ctaaccaccg atgcgttcga
     8521 gtcagtgtgg gccaccgtca gcttcagccg ggggctcttc ggtcatgcgc cggcccctgc
     8581 tttcattgag aaggtgctgc gcgacttgtc tttatgggaa aaacgcaaaa acaagatcat
     8641 gacgttgtcg ggcggcatga agcggcgcgt gatgatcgcc aaggcgctgt cgcacgagcc
     8701 ggagattctt tttctcgatg agccgaccgc aggcgtggac gttgagttgc gccgcgacat
     8761 gtgggcgttg gtgcgaactc tacgggaaag cggcgtgacc atcatcctga ccacccacta
     8821 tattgatgag gccgaggaga tggccgaccg catcggggtg atcaacaaag gaggactggt
     8881 ggtggttgaa gagaaagtcg agctgatgaa aaaactcggc aagaagcagc tgaccctgag
     8941 tctcctcgac ccattgccat cacttccgct ggaatta
//
LOCUS       DASUTG010000204         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_774081, whole genome shotgun sequence.
ACCESSION   DASUTG010000204 DASUTG010000000
VERSION     DASUTG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774081"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VF089_04575"
     CDS             complement(<1..836)
                     /locus_tag="VF089_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6173267.1"
                     /translation="MKGLGGIILFVIFLVLISLRQLISLYVDWLWFEEVGYTQVFTTS
                     LFFKTVLALLGAGIFALLIYANARIAARTVRGIRFLDQENFLDLPRPELIDPLVRRLL
                     LPAALVLGLFAAPQAASHWESFLLLLNPTSFGLEDPLFNKDLGFFVFQLPALRTLYNW
                     LMTALALSLVVTAVIYVLYRGVQYSPRGIFLAPRARIHLLVLIAALLAVKAAGYVLDS
                     YDLLYASGGAAFGAGYSDVYVSLPALRILTVAALIAAALSAAQIARPGFGYLLVGIAG
                     LVL"
     gene            complement(1014..1859)
                     /locus_tag="VF089_04580"
     CDS             complement(1014..1859)
                     /locus_tag="VF089_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6173268.1"
                     /translation="MAEQAAEEQLLDVSVAESSAAYKFYLNNEKKILGTSAVIIFLTA
                     WELVGNVLGLINPMFMSAPSLIFKAAVQMFSSGEIYNDLYVSGLEFGWGYLLSVAVGV
                     PFGIACGWYKKFAYIFDPFINAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPI
                     LINARDGVKTTPYNLLTAARSFGASEWQIFKSVVLPSTLPFILTGLRLGVGRALIGVM
                     VGELYAATAGIGFMITVAGATFQTDKVFVGVLIFAISGMIAMELLTRLESRFDKWRPK
                     VGAVA"
     gene            2088..>3046
                     /locus_tag="VF089_04585"
     CDS             2088..>3046
                     /locus_tag="VF089_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX6173269.1"
                     /translation="MKILFVTPPMGNWAPWGDRHVAVNSLHAQLAAFVREQNAAEVEV
                     LDCRALALDDDGMLEKIKQSRPGVVFFGNMIAAAGGAAQLNRFHAAMRRIKDVLPDTI
                     TVGGGLMYTAVPQKIMADNPQLDFAIVGVFGDNEYTLCELLETLKMPSGQFGAIKGLA
                     YRENGEVVLNQPRALISNLDDLPLPAYDLFPMDRYCGYSVIPNYNETVTSRGCEGACH
                     FCYEWWLVDPRNPRDFSSHRTKGGKKVAQEMELLNRKYSVKALTFMDDDFNSDRRKMV
                     DMLEELEKKKLDMSWFMLSRAQNLIRDADLIPRLRKVGLYQVL"
BASE COUNT          838 a          780 c          799 g          629 t
ORIGIN      
        1 agaactaacc cagctatgcc aacaagcaga tagccaaacc ccgggcgagc aatttgagcg
       61 gctgagagcg cagccgcgat caaagcagcc accgtcaaga ttctcaaggc cggaaggctt
      121 acataaacat cgctataacc agcgccaaaa gctgcaccgc cggaagcgta cagcagatcg
      181 tacgaatcca agacatagcc cgctgcttta acagcgagta aagctgcgat cagaaccaac
      241 aggtggattc tggcgcgcgg agcgaggaaa ataccgcgcg gactatactg cacacctcgg
      301 taaagtacgt aaatgaccgc agtcactacc agagaaagcg ccaaagccgt catcaaccaa
      361 ttgtacaggg tgcgcaacgc cggcaattga aacacgaaaa agccaagatc tttattgaat
      421 aacggatcct cgaggccaaa ggacgtcgga ttgagcaaca gcaggaatga ttcccaatgg
      481 ctggctgcct ggggcgcggc aaaaagcccg agaaccaatg cagcaggcag gagcaatcgc
      541 cggactaagg gatcgatgag ctccggtctc ggcaggtcga gaaaattctc ttgatccagg
      601 aatcgaatgc ctctgaccgt tcgagctgct atcctggcat tagcgtaaat gagcagcgca
      661 aaaatacccg cgccaaggag agccagaaca gttttgaaaa acagactggt ggtaaaaacc
      721 tgagtgtaac cgacttcttc aaaccagagc caatcgacat aaagcgaaat gagttggcga
      781 agcgaaatca gcaccagaaa gattacgaat aaaattatcc cgccaagtcc cttcatgaga
      841 ttgccccagt ccagttagat ttagaaaatg cagctaggaa aagaagttcc gcaggaataa
      901 catcgaaacc gattctaaga gccggcgatc gagggttgcg gaaagagata gggatggaat
      961 aggtggctcc cgcgattcga gagccacctg tcgatgatgg gtgaacagct gctctaagcg
     1021 acagcgccaa ccttaggacg ccacttgtca aagcgggatt ccagtcgggt caggagctcc
     1081 attgcaatca ttcccgagat cgcaaagatg agtacgccca cgaatacctt atcggtttga
     1141 aacgtggcac cggcgactgt aatcatgaag ccgatacccg ccgtggccgc ataaagctct
     1201 ccaaccatga cgccgatcag cgcccggcct accccgagtc ttaagcccgt aagaataaaa
     1261 ggaagagtcg agggcagcac aacgcttttg aaaatctgcc attctgaagc gccgaagctc
     1321 cttgcggcag tcaaaaggtt gtatggagtg gtttttacgc cgtcgcgcgc attgatcaag
     1381 atcggaaaga cggcgccgag aaaaatgatt ccgactttgg acaagatgcc gatcccgagc
     1441 cagatgatga ctagcggcaa gagcgcaact cgaggcgtcg cgttcatggc attgataaag
     1501 gggtcgaata tgtaggcaaa cttcttgtac caaccgcagg cgatgccaaa aggcactccg
     1561 acagcgacag ataacaagta gccccagccg aactcaaggc cgctgacgta taaatcgttg
     1621 tagatttctc ccgatgaaaa catttgaacc gccgctttga agatcagcga cggcgcgctc
     1681 ataaacatcg ggttaatcaa cccgagcacg tttcctacta actcccaggc ggttaaaaat
     1741 atgatgaccg ctgaggtgcc cagtatcttc ttttcattgt tgagatagaa cttataggcg
     1801 gcagatgact cggctacgct gacgtctaga agttgctcct ctgcggcttg ttcagccata
     1861 tctttcctcc taatggttta ttcagtcacg gtcggccagc accgcgaacg tgtaacaaac
     1921 gggttagttc ttgtcaatac aaaggtcggc acgacccaaa gcctgttttc agagcgacga
     1981 ctttaggacc ctccgaaagc atcccatggg cgaatcttga caatcgttaa agccttaaat
     2041 atttttggcc tgaaccaaca aattatcctt ccgagccagg gagatacatg aaaattcttt
     2101 ttgttacgcc gcccatggga aattgggccc cgtggggtga tcgccacgtg gctgtcaatt
     2161 cactgcacgc gcagcttgcg gcattcgtta gagaacagaa tgccgctgaa gtggaagtac
     2221 tcgactgccg cgcgcttgca ctcgatgacg atggcatgct cgagaaaatt aagcaaagcc
     2281 gccccggggt ggtgtttttc ggcaacatga tcgccgcggc aggcggtgcc gcgcagctca
     2341 atcggtttca tgcagcgatg aggcgaatca aggacgtctt acccgacaca attaccgttg
     2401 gcggcggtct catgtacacc gctgttccgc agaagatcat ggccgataat cctcaactgg
     2461 acttcgcgat tgtcggcgtt ttcggagaca acgagtacac tctttgcgag ttgttggaga
     2521 ccttgaaaat gccatccggg caatttggcg cgatcaaggg gctggcttac cgcgaaaatg
     2581 gagaagtcgt tctcaatcag ccacgcgcgc ttatttccaa cctcgacgat ttgccactgc
     2641 cggcttacga tctttttccc atggaccgct actgtgggta ttcggtcatt ccaaactata
     2701 acgaaacggt aacgtcgaga ggctgcgaag gcgcttgcca cttttgttat gaatggtggc
     2761 tggtcgaccc gcgtaaccct cgtgatttca gctcgcaccg gaccaagggc ggcaagaagg
     2821 tcgcccagga gatggagctc ctcaatcgta aatatagcgt taaagcgctt accttcatgg
     2881 atgatgattt caattccgat cgacgaaaga tggtcgatat gctcgaagag ctggaaaaga
     2941 aaaagttgga catgagctgg tttatgctca gcagagcgca gaatttgatt cgtgatgccg
     3001 acctgattcc gcgtttgaga aaagtcggtc tctatcaggt gctgat
//
LOCUS       DASUTG010000205         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2109189, whole genome shotgun sequence.
ACCESSION   DASUTG010000205 DASUTG010000000
VERSION     DASUTG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109189"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1082)
                     /locus_tag="VF089_04590"
     CDS             complement(60..1082)
                     /locus_tag="VF089_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6173270.1"
                     /translation="MEHLSPARIMEVGLGFWPAKVLLSAIELGVFTELGANSMTGREL
                     QVALHLHPRANPDLFDALVALRFLHREGDGPEARYLNTADTALFLDRRSPQFIAGFLE
                     MANSRLYRFWGDLTEGLRTGKPQNEIKHTGAPMFAELYSRPERLEQFMDAMSGISAAN
                     FQAFADKFNFSGYRTLCDIGGATGQLSMLVAKKHPHLRCTSVDLPAVTRIAERKIAAA
                     GLADRVSAKPLDFFAEPLPKAHVITMGMILHDWNLEKKMHLVRAAYDALPAGGAFVVI
                     ENLIDDARRENAFGLLMSLNMLIEFGDAFDFTAADFFGWCREAGFSRTEVIPLAGPAS
                     AGVAYK"
     gene            complement(1221..2243)
                     /locus_tag="VF089_04595"
     CDS             complement(1221..2243)
                     /locus_tag="VF089_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6173271.1"
                     /translation="MARLNTHGSVLVFVTLAITLLLIMVGMGLDTGMLSYVRSQAQPA
                     VDAAALAAVSGLVSGQAEVESRVAAYNATNDYTGQTGSSLGATNITPIFYNGSSIELA
                     ASYGTANGVRVGLEAAGSNPYAGNTKSSIASPLFLTPLLNLMGYSASATANLNVTATA
                     VLQARPQLPFTIVGCTVGDFTASWSQTPSTTDNSAFTSFTLPSANVNTMRDMVSASCG
                     AIPPVGIGSCINLNNGQDTPVLQEIIQAFGPRNAPFTDPPKDPEDCFLVPVIPNAPVD
                     GNLNQCRPMESLARLCITNVNAPSQPDFSPPMSISGRITDCNVPIYGVTQCSIPVLVR
                     DTKSGM"
     gene            complement(2270..2764)
                     /locus_tag="VF089_04600"
     CDS             complement(2270..2764)
                     /locus_tag="VF089_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6173272.1"
                     /translation="MDLPAKRDCDPNNHLIAGRRGSNGQSIVELTLIVPLVLATLYIP
                     ADFGIAFFTAQMVQNATREAARIGASMNPFVAATIENEATKRLPGGKFVTTSVSATLN
                     GSSTSTCMRRVVVTVSGSYNLFWYRLLNLMLPGTVTDTSLPISRSTAMRYDSQALTNT
                     GSCT"
     gene            complement(2748..3431)
                     /locus_tag="VF089_04605"
     CDS             complement(2748..3431)
                     /locus_tag="VF089_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6173273.1"
                     /translation="MAVLLWELVIVAAFVLVAVGALAYVLSNLLLGAIHKRAGRRFAG
                     LISARARKRSQRQPGDGLRTRFIRIISGRSIFSPWVPKRTARKHLPRKARGQALVEFT
                     LMFVLFLAIIWIPADFGLAFYSGQLAQNASREGARLAAATNPFDAGNIQSQVINRMDS
                     AVLKNITANIVPPACDPALNMQVVTVTVAGEYNFYFYQLLRLLGFSAPDNVVISRTTT
                     MRYEYGFTC"
BASE COUNT          909 a         1019 c         1068 g          860 t
ORIGIN      
        1 gatcggattc tcggaattga gaggtctcct tgtgaaactc gcgtgaaacc ccggtgcaat
       61 tacttgtacg cgacaccggc acttgcgggc cctgccaaag gaatcacctc ggttctgcta
      121 aagcccgcct cacgacacca gccgaaaaaa tccgcggccg tgaagtcgaa ggcatcgcca
      181 aactcgatca acatattgag cgacatcagc agcccgaaag cgttttcacg tcgcgcgtca
      241 tcgatcaggt tctcgatcac cacgaaagcg ccgccggcag gtaaggcgtc gtaagctgcg
      301 cggacaagat gcattttctt ctccagattc cagtcgtgga gaatcatgcc catggtaatc
      361 acatgcgcct ttggcagcgg ctccgcgaaa aaatcaagag gtttcgccga gactcgatcg
      421 gcaagtcccg ctgccgcgat ctttcgctcg gcgatccggg tgaccgccgg aaggtcgacc
      481 gaagtgcagc gaaggtgagg atgtttcttg gccaccagca tagacaactg cccggtagca
      541 ccgccgatgt cacagagggt gcggtaaccg gagaaattga atttgtcggc gaaagcttga
      601 aaattggcgg cggagattcc gctcattgca tccataaatt gctcgagccg ttccggcctg
      661 ctgtagagct ccgcgaacat cggcgcgccg gtgtgcttga tttcgttttg cggcttgccg
      721 gtccgcaggc cttccgtgag gtcaccccag aatcgataca ggcgcgaatt ggccatttcc
      781 agaaagccgg cgatgaactg cgggctgcgc cggtcgagga acagcgcagt atctgcggta
      841 ttaagatagc gcgcctccgg cccatcgcct tcccggtgta aaaaacgcag cgccaccagg
      901 gcgtcgaaga gatcggggtt cgctctcggg tgcagatgaa gagcaacctg cagctcgcgg
      961 ccggtcatgg aatttgcgcc aagctctgta aaaaccccca actcaatggc ggaaagcagt
     1021 accttggccg gccagaagcc caggccaacc tccatgattc gagctggaga taaatgttcc
     1081 atcgtgatgc tcctttctgg acggatcacg ggtcaacagc tcatgtcagc gtggtgtggg
     1141 ttcgaaacgg agtgccgttg gaccgtgctt aagaaagatc ccgtaacgtg tctttctcga
     1201 cgatgcaagt ccctcggcag ttacatgccg gatttcgtat ctctcacgag aacggggatg
     1261 gagcactggg ttacgccgta gatgggaaca ttacagtcgg taattcttcc cgatatgctc
     1321 atcggtggtg aaaaatccgg ctggctcggt gcgtttacgt tggtgatgca caatctggcc
     1381 agcgattcca tggggcggca ttgattcaga ttcccgtcaa ccggagcatt aggaatgaca
     1441 ggcaccaaga aacagtcttc agggtctttt ggcgggtcag tgaacggagc atttcttgga
     1501 ccaaatgctt gaatgatttc ttgtaaaacg ggggtgtcct gcccattgtt gagattgata
     1561 cagctaccga tcccaacggg aggaatagct ccgcaactcg ccgaaaccat atctctcatc
     1621 gtgttcacgt ttgccgaggg tagggtgaag ctggtaaacg cggaattgtc ggttgtactc
     1681 ggggtttgac tccatgaggc ggtgaagtca ccgaccgtac agcccactat tgtaaacggt
     1741 aactgtggtc gtgcctgaag tactgcggtt gccgtaacgt ttaggttggc tgtggcagaa
     1801 gccgagtatc ccatgaggtt caagagaggc gttagaaata acggtgaagc gatcgagctc
     1861 ttcgtgttac ccgcataggg attgcttccg gccgcttcca atccgactcg caccccatta
     1921 gctgtgccgt aggatgcggc gagctcgatt gaggatccat tgtagaaaat aggagtgata
     1981 ttagtcgctc caagactgct gcctgtttgg ccggtataat cattggtcgc gttgtaggca
     2041 gcgactcgcg actccacttc tgcttggcct gagaccagcc cggacacggc agcgagtgca
     2101 gccgcatcca ctgcgggttg cgcctgactc ctaacgtagc tcagcatccc cgtatccaat
     2161 cccatgccga ccatgattag caacagggtt atagccagcg tgacaaaaac taaaaccgag
     2221 ccgtgggtgt tcaagcgtgc cataggagct cctttataaa gcgccgccat caagtgcagg
     2281 aaccggtatt cgtaagcgcc tgggagtcat agcgcatagc ggtgcttcgg cttattggca
     2341 gcgaagtgtc tgtcaccgta cccggcagca tcagattcaa cagccggtac caaaacagat
     2401 tgtaactacc gctaacggtg accactactc ttctcatgca agttgaagtg ctactgccat
     2461 ttaacgttgc cgagacgctg gtggtcacga acttcccacc tggaagacgc ttcgtcgctt
     2521 cattctcgat tgtcgcagca acgaatggat tcatcgaggc gccgattcga gcggcttccc
     2581 gggtggcatt ctgaaccatc tgggcagtga aaaacgcgat gccaaaatct gccggaatat
     2641 ataaggtcgc gagcaccaga ggcacgatca aagtcagttc gacgatactt tggccgtttg
     2701 agcctcgcct gcccgcgatt aaatgattgt tcggatcaca atcgcgctta gcaggtaaat
     2761 ccatattcat acctcatcgt agttgttcgg ctgatgacaa cattgtcggg tgcagagaag
     2821 cctaggagcc gtagtaattg atagaaataa aagttgtact cgccggctac cgtaaccgtg
     2881 accacctgca tattaagggc gggatcgcag gccgggggaa ctatgttcgc agtaatgttc
     2941 tttaaaacgg cgctatccat tctattgatc acctggctct gtatgttccc ggcgtcgaac
     3001 gggtttgtgg cggcagccag gcgggctcct tcgcgggagg cattctgcgc taattggccg
     3061 ctataaaacg ccagcccgaa atccgcaggt atccaaatga tggcaagaaa gagcacgaac
     3121 atgagggtaa actctactag agcttgcccc cttgccttgc gaggtagatg ttttcgcgct
     3181 gtacgcttgg gaacccaagg tgaaaagatc gatctaccgg agatgatcct aatgaaacga
     3241 gtccttagtc cgtcgcctgg ctggcgctgc gatcgtttcc gcgcgcgagc agagatcagg
     3301 ccggcaaatc tccttccggc ccttttgtga atggccccca gaagcaaatt cgacagtaca
     3361 tacgcaagag cgccgacagc tacgaggacg aaggctgcca caatgaccag ttcccaaagc
     3421 agtactgcca tgtgagtatc tcccgggttc tgaataacaa ccgttcagaa gcggcgggta
     3481 aatatcacaa ggcctttact tttcaagagg cgggtttgtg gggattcacg gagacgctag
     3541 ggcatcgagt aagtaaccga tcgcctctga attgacgaga aggctcacgg tagcgaacga
     3601 gattaagtca gattttcaat cacattccac caatttttga gtatcggccg gctggttaga
     3661 tatgccgcca tcccaacttc caatgccatc accgccagct ctcgttcgtt acagcccgac
     3721 agcgcaacaa caggtatttt agcagtctga ggatttgttt ttatggtttc agcggtctct
     3781 aacccaccga aaatcaggca gtgagagata aactagcacg gttcgtttta gtgcaacgcg
     3841 catgtggaga gcggct
//
LOCUS       DASUTG010000206         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_33807, whole genome shotgun sequence.
ACCESSION   DASUTG010000206 DASUTG010000000
VERSION     DASUTG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33807"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..542
                     /locus_tag="VF089_04610"
     CDS             159..542
                     /locus_tag="VF089_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6173274.1"
                     /translation="MRASSFDTLRLRHHWQPLIFLTMDFKDGSTLIADLTTVGRKTGQ
                     PRTVELRLVYHNGSYYASSSKVSGKHWCQNMLKNPAVELTIMGKTISCIARQVTEDKL
                     RKEILSLRDSPPRMERVVFEITPQS"
     gene            complement(724..1617)
                     /locus_tag="VF089_04615"
     CDS             complement(724..1617)
                     /locus_tag="VF089_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6173275.1"
                     /translation="MMTTDLQTGMAYALLAGTLWGMGPLALKRALKHTSVGTATLTEQ
                     YLSVLLLGVLAAYHGELAHLDFTSRAFWAFFLAGAIGASFGKIFYYKGIDRVGASKAT
                     SVKNSSPILTTFLAVVFLGEDMSWWILAGVLLIVAGIAALTRTEKASTGGPNRLQYFL
                     FPLLAAICFGINPIFKKIGINAVSLPILGAFITQATALLVMLTLGRFFSVKAKWEPMP
                     IKALGLVTISSLTEALGSLFTFFALIHAPAVLVSPMWRISPLVTFALVRFTLRGIEVV
                     TLRDGLAAGLIVGGVFVLTRG"
     gene            complement(1636..1854)
                     /locus_tag="VF089_04620"
     CDS             complement(1636..1854)
                     /locus_tag="VF089_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173276.1"
                     /translation="MRMTLITGMVLALAIGLSACGKDDKDSAARDAEIQKKLQEGSQR
                     ERQMYEGMQKGVENLEKQSSPPKQETTP"
     gene            2041..2817
                     /locus_tag="VF089_04625"
     CDS             2041..2817
                     /locus_tag="VF089_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX6173277.1"
                     /translation="MAQAPAPAGKPKVSSTLLVRKPDAWKPLDSGIEHRKMTLERSDP
                     SYSFDLNLVRFGRNRVFPRIISSGQFQLKSADAKTLGQRSGALVTINANYFDEKGKPL
                     AFLKTAIGAINRNVSKHALYTGVFGVRDSIPFIVHRDDFQLTEAQEAIQSGPLLLRQG
                     APMQTIPGAGRYSRRAVIGIDKQQRLIVGVTDTVLGGLSFAELQELFSRPEWQLETTE
                     LLNLDGGGSAQLYIHTRSHEEFISGTSEVPVVIGFFKTAN"
     gene            complement(2814..>3849)
                     /locus_tag="VF089_04630"
     CDS             complement(2814..>3849)
                     /locus_tag="VF089_04630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6173278.1"
                     /translation="VAVYVLTGIWRGSARSSEAAMKYFLMGSFATGFLLYGIALIYGA
                     TGTTNLTQVSAYLLQPQDQWPGYLSVGGLLLLVGFAFKVGAVPFHFWVPDVYEGAPTP
                     VTGFMSVAVKAAAFAAWARILMHQLSPLDSDWAYAVRVLAIATMTLGNLLAISQSSVK
                     RMLAYSSIAHAGYILIGIVAGEEWGSTSVLFYLATYVPVTFGAFAVITALTEKDRPKE
                     EYGHFAGLGFKRPFLAMAMTFFMLSLAGFPPLAGFTGKFHIFRSAIAAGYTDLAIIGV
                     LNSLLSLIYYLRVIVMMYMSEGGVQGKSLAESPSLYVAVALATVATIYLGILPTRAVE
                     WSRIAYSTLF"
BASE COUNT          944 a         1078 c         1024 g          803 t
ORIGIN      
        1 tacccttggc cttcggcagc gcgcgcacag cggcgacact cctgccgaag cgaatcatca
       61 ttggtgaaat tcccgttgct tgttgccgag ccatgaatac ctcccctacg attaaatgaa
      121 cccgaaccga ataaaccccg aaagcttccc atgcaatagt gagagcgtca agcttcgaca
      181 cgctgcgctt gcgccatcac tggcagccgc ttatattttt gacaatggat ttcaaagatg
      241 gatcgacttt gatagcggat ctgacaacgg tcggacgaaa aaccggccag ccgagaaccg
      301 tagagctacg cctggtttat cataacggca gttattacgc gtcatcgagc aaagtaagcg
      361 gcaagcactg gtgccagaac atgctcaaaa acccggcggt cgaactaacc atcatgggca
      421 aaacaatttc ttgcatcgcc agacaagtca ccgaagacaa actgcgcaag gagattcttt
      481 cgctgcgaga ttcgccgccg cgtatggagc gagtggtttt tgaaataacg ccgcaaagct
      541 gattctggtt caagccattc aaatagttca agccgttcaa gtcgtttgga cccggagaaa
      601 caagaccaat ccggtttgtt tttgctcctg accatggcgg cagcttgttt ttgactggcg
      661 ccttcagcgt ctcccacgtt ttgatcgttt tgaactgctt gaacggcttg aactctcgta
      721 attctatccg cgagttaaaa cgaacactcc gcccacaatg agcccggccg ccaggccgtc
      781 gcgtaatgtc accacttcaa tgcctctcaa agtaaagcgc accagggcga acgtgactaa
      841 cggtgaaatg cgccacattg gcgaaaccag taccgccggc gcgtgaatca atgcgaagaa
      901 cgtgaacagt gatccgagag cttcagtcag gctagaaatt gtcactaacc cgagcgcctt
      961 aatcggcatc ggctcccatt tcgctttgac tgaaaaaaac cgtccgaggg tgagcatcac
     1021 gagcaatgcc gtggcttgag taatgaaagc gccgagaatc ggcaggctca ccgcgttgat
     1081 gccgatcttc ttaaatatcg gattgatgcc gaaacaaatc gctgcgagaa gcggaaacaa
     1141 aaaatattgg agacgattgg ggcctccggt cgaagctttc tcggtgcgag tgagagcggc
     1201 gatgccggcg acaatcaaca ggactccagc caggatccac caggacatat cttcgccaag
     1261 gaagacgact gcaagaaagg tcgtcagaat tggtgaactg ttcttcaccg aggttgcttt
     1321 cgacgcgccg actcgatcga tccccttgta gtagaaaatc ttgccgaagc tagcgccaat
     1381 ggcgccggca aggaagaacg cccaaaaagc gcgactggta aaatccaggt gagcgagttc
     1441 gccgtgatac gccgccagca cgccaaggag caaaacggat aagtattgct cggttaaggt
     1501 cgccgttcct acgctggtgt gcttcaaagc gcgcttgagc gcgagcggac ccatgcccca
     1561 gagggtcccg gccaacaagg cataggccat gccggtttgc agatccgtcg tcatcatcga
     1621 tggcagagag tctagttacg gagtcgtctc ttgttttggc ggcgacgact gcttttccag
     1681 attttcgaca cctttctgca ttccttcata catctgtctc tctcgttgcg atccctcctg
     1741 aagcttcttt tgaatttctg cgtcccgcgc ggcactgtcc ttatcatctt tgccgcatgc
     1801 ggataatccg atggcaagag cgagcaccat tccggtgatt aacgtcattc gcatattcgt
     1861 gggtccttct tcatttattg ggtagtcgag ttacttagca aaaaaaattg cctagcggca
     1921 actcgttaat ctttgctttc cttcaccgta tcggttctgg taaactcagc caacgtgatg
     1981 cttcgctcgt tttccttgct gattctgctc tcttgcctca cgctggcagc aactttcagt
     2041 ttggcacaag cgcccgcgcc ggcaggcaaa ccgaaggtga gctcgacttt gttggtgcgc
     2101 aagcccgacg cttggaaacc tctcgactcc ggcattgaac accgcaaaat gaccctcgag
     2161 cgttctgacc caagctattc gtttgatttg aacctggtcc gctttggccg taatcgggtc
     2221 tttccgcgca tcatcagcag tggccaattt cagctcaaga gcgccgatgc gaaaacgctg
     2281 ggccagagaa gcggcgccct tgtcaccatc aacgccaact acttcgacga aaaaggcaag
     2341 cctctagcgt tcctgaaaac ggcgatcggg gcgatcaatc gcaacgtctc taaacacgct
     2401 ctctataccg gcgtctttgg tgtgcgggat tcgatcccct tcattgtgca tcgtgacgat
     2461 tttcaactca ctgaggccca ggaggcgatc cagagcggac cgttgttgtt aagacagggc
     2521 gcgccgatgc aaacgatacc gggcgcgggc cgatacagcc ggcgcgcggt aatcggcatt
     2581 gataaacagc agcggcttat cgtcggcgtc acagatacgg tccttggcgg gttgagtttt
     2641 gccgaactgc aggaactttt ttcccggccg gagtggcaac tcgagacgac ggaattgcta
     2701 aatctggacg gcggcggatc agcgcagctt tacatccaca ccaggagcca tgaagagttt
     2761 atcagcggca cctcagaggt acccgtggtg atcggcttct tcaaaaccgc caactaaaaa
     2821 agcgtggagt aagcgatccg gctccattcc acagcccggg tcggtagaat cccgagatag
     2881 atcgttgcta ctgtcgccaa ggcgacggcg acgtagaggg agggagactc ggcaagggat
     2941 tttccctgca ccccgccctc gctcatgtac atcatgacga tcacccgcaa atagtagatc
     3001 agcgacagca aactgttgag cacaccgata attgcaagat cggtgtagcc ggcggctatg
     3061 gctgaacgga aaatgtgaaa cttgccggtg aatcccgcta gcggaggaaa gcccgccagc
     3121 gagagcatga agaaggtcat tgccatcgcc agaaacggtc gcttgaaacc tagaccggca
     3181 aagtgtccgt actcttcctt gggccggtcc ttttcagtca atgccgtgat caccgcgaaa
     3241 gcgccaaaag tcaccggcac atacgtggcg agataaaata acacggacgt gctaccccac
     3301 tcctcgccgg cgacaatgcc gatcaatata tagccggcgt gagcgatgct cgaataagcc
     3361 agcatgcgct tgacgcttga ttgggagatc gccagcagat tgccgagagt catggtggcg
     3421 atggcaagca cgcgcacagc ataagcccag tcgctatcca ggggcgaaag ctggtgcatt
     3481 agaatgcgcg cccaggctgc aaacgccgcc gctttcacgg ccaccgacat aaagccggtc
     3541 accggggtcg gcgctccctc ataaacatcc gggacccaga agtgaaacgg caccgcgccc
     3601 actttgaatg cgaagccaac gagaagcagc aaaccgccca ccgaaagata tcccggccac
     3661 tgatcttggg gctgcaaaag ataggcggaa acttgggtca gattggtcgt cccggtggcg
     3721 ccgtagatca acgcaatgcc gtaaagcagg aagccggtag cgaaggagcc catgagaaaa
     3781 tacttcatgg ctgcttcgct tgaccgagcg ctgccgcgcc agatgccggt cagcacataa
     3841 acggcgacc
//
LOCUS       DASUTG010000207         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2109571, whole genome shotgun sequence.
ACCESSION   DASUTG010000207 DASUTG010000000
VERSION     DASUTG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109571"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1396
                     /locus_tag="VF089_04635"
     CDS             161..1396
                     /locus_tag="VF089_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173279.1"
                     /translation="MKRQLIEVAPAMIFLSLLFPTSELVHARQPVASFYYDDRGQVIR
                     QARDTNADGKMDRWSHYNGQGQIERIEQDVNFDGKPDTLVYYENGKPRRQEVASNNDG
                     QIDLWYLFDPDGKVERKGQDPNRTGKPTLWVYYQDGQPLRAEEQNSSSGAVNRSVYFR
                     DGKISRVEEDTNADGRPDSFAFYENGQLIKKELDRKHTGKTDLWGYYLDNRLVKQEED
                     LRGDGAVSRIVYFQNETVVRREEDTMGRQRMDIIETFAQGKLAKRLRDTKGSGNWDAV
                     YIFENDQMVREERDTDADGIFDLRIFYADGQIKRQEGDTNRDRRVDVWVTFENGERVE
                     QLEDQHSRGTISARYTFKNGQVIGQEPVADAQPPSMALPFGPAEDELRSMAASTGIPL
                     DEPRATAVAAGLQSDSRTK"
     gene            1580..2560
                     /locus_tag="VF089_04640"
     CDS             1580..2560
                     /locus_tag="VF089_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173280.1"
                     /translation="MTGSQRWNHAWTKAMEGAFVGLNIGGPFGGAGGLALGLLTGLIT
                     ADSHYGAVNAQIQSEQEKDRQLEAAIEEELQRQAGFENQVAQATPTTGGATQLGPAET
                     PVEQGGAAPTGPAMRPATGNIAVASVDKSSRPTPPTQFKNVEVKDINGDGVPDLWIYH
                     NPRNPGEIVRQEEATKYDGNVDAWSYFKDGKLVRRDVDSQGQGRPDTVFYYADDKIAR
                     EERDESGTGRIIYRAFYQNGRLAQIEKDTRGKGKIDFWIHYDTSRAEQIMIKEEQDMD
                     GDGIADLWTYYEDGRIVRRDVSAVGLELLAKQEQISLPIGDVKEIQVPAN"
     gene            2628..3584
                     /locus_tag="VF089_04645"
     CDS             2628..3584
                     /locus_tag="VF089_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX6173281.1"
                     /translation="MPSLLLHAIFLALVFRAASLSVTKPALDPPISVQLVEVNEGGSS
                     NKSIGSSKGPGGPRTSPKLGNPIAPAQRTGKLDSGSLDASRASDAVEATAAPTPMALP
                     GPKVLSSDSSREAVNVKETSPDSLVRLPTKEAATNLSGGMAVDPQTSEKSLAALKNTY
                     PGAGIKALREGEQIPGSLKGTGTGPGPYGVPGGSRTGSGLSGGGSGSGEGGGSHTGLK
                     GIGNSDYNQYLEQLKRRVESVWKYPDDVTGVQKVAVRFSLDRAGKLTLAEVLDSSDSR
                     LNTSAVEAIKRASPFPPIPESLKDLANEPMIIRFEVAIRVRG"
     gene            3668..4297
                     /locus_tag="VF089_04650"
     CDS             3668..4297
                     /locus_tag="VF089_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX6173282.1"
                     /translation="MMEQQGLNLIDIIHKGALATYPLILLSIVSVTVVFERLWSLRNI
                     RSTTLRIAESLIDPVQKGQRDLALAICKQNSHCPAGRIFHSVLERAESHQPETGSNVA
                     VEAMFEETQHLRKHLWILGTVASSAPFIGLFGTVVGIIKAFESMAVSGTGGFAVVAAG
                     ISEALVATALGLGVAIIALVFYNYFQTRIGSLTALFRIQVARILRHFNG"
     gene            4308..>4585
                     /locus_tag="VF089_04655"
     CDS             4308..>4585
                     /locus_tag="VF089_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX6173283.1"
                     /translation="MALTPLGNEESSDIVAEINITPLTDIFLVLLIIFMITSSAMIES
                     GGKVNLPKAVATQSESRGTTVTLTPKREIFVNQKQITEDKLEAALKDAL"
BASE COUNT         1148 a         1185 c         1304 g          948 t
ORIGIN      
        1 ttcctgattc gggaaaatct ctgagtgcaa cgattcgcgg cttaggaaaa aaattaagcg
       61 ccgtcgccga atgcaagtct gtgagatcag cgagtccaat gccccacaac tacgtttcca
      121 agactgtgat cgtgccgtag cagttcggag ttctcgagcg atgaagcggc agctaatcga
      181 agtcgcgcca gctatgattt tcctctcgct gctatttcca acctccgagc tcgttcatgc
      241 ccggcaaccg gtagccagct tttactacga tgaccgagga caggtgatcc gtcaggcgcg
      301 cgacaccaac gcggatggca aaatggatcg ttggagccat tacaatgggc aaggacaaat
      361 agaacgaatc gaacaagacg ttaatttcga cggtaagccg gatacgttgg tttattatga
      421 gaacggcaag ccccgacgcc aggaagttgc cagtaataac gacggacaaa tcgacctctg
      481 gtatcttttc gatccagatg gaaaagtcga gcgcaaaggc caagatccaa acagaacggg
      541 taagcctacc ctgtgggttt actatcaaga cggtcagcct ctacgggctg aagaacagaa
      601 cagttcttcc ggcgcggtta atcgctcggt ttattttcgc gacggtaaga taagtcgggt
      661 tgaggaggac acgaacgccg acggcaggcc ggacagcttc gctttttacg agaacggcca
      721 gttgatcaag aaagagctgg accgcaagca caccggcaaa acagatctct ggggttacta
      781 tctagacaat cggctggtga agcaggaaga ggaccttcgc ggtgacggcg cagtgagccg
      841 gattgtttat ttccaaaacg aaaccgttgt acgccgggaa gaagatacca tggggcggca
      901 acgaatggat atcatagaaa cgtttgctca aggcaagttg gccaaacgtt taagggacac
      961 caagggctca ggaaattggg acgcggttta cattttcgaa aacgaccaaa tggtacgtga
     1021 agaacgggat acagatgcgg atggcatctt cgacctgcga atcttctatg ccgatggaca
     1081 aatcaagcgg caggaaggtg ataccaaccg agaccggcgt gtcgatgttt gggttacgtt
     1141 tgagaacggg gaacgtgtcg agcagcttga ggaccagcat tctcgtggca cgatcagcgc
     1201 gcgctacacc ttcaagaatg gacaggtgat cggccaagag ccggtggcgg acgcgcaacc
     1261 tccttcgatg gcgctcccct tcgggccggc cgaagatgag ctgcgatcca tggccgcttc
     1321 cacgggcata ccattggacg aaccgcgggc gactgccgta gctgccgggc tacagtcgga
     1381 cagcagaact aagtagaaca gatcgggcag gtagaagatg aacgggcgat ttggatcgat
     1441 aacatgcatg atgctattga cgctgctagg atgtgaacct gttgccgttc aatacccgcc
     1501 tggctctgtt caatcgcctt actccggtgc tgccgtaagc aacgcaagcg gcgcccctcc
     1561 gtacccaacg caaccacagg tgaccgggtc gcagagatgg aatcacgcct ggaccaaggc
     1621 tatggagggc gcctttgtcg gcctcaatat cggcggtcct tttggcggcg ccggcggctt
     1681 agccctgggg ctgcttacgg ggctgatcac cgccgactct cattacggcg cagtgaatgc
     1741 ccagattcag tccgaacagg aaaaagaccg gcagcttgaa gcagcgattg aggaagaact
     1801 gcaacgccag gcaggctttg agaaccaggt tgctcaagca actcctacaa ccggcggcgc
     1861 tactcaacta ggtccagccg aaacccccgt cgagcaagga ggtgcggcgc ccaccggccc
     1921 agccatgcgc ccggcaaccg gtaatatcgc agtggcctcg gttgataaga gttctcggcc
     1981 tacgcctccc acgcagttta aaaatgtcga agtgaaagac atcaatggag atggggtgcc
     2041 ggacctctgg atctatcaca acccgcgaaa cccgggtgag atcgtgcgtc aggaagaggc
     2101 gacgaaatac gacggcaacg tcgatgcctg gagctacttt aaagatggca agctggtccg
     2161 gcgcgacgtg gacagccagg gtcagggaag acctgatact gtcttttact atgccgacga
     2221 taagatcgcc cgagaggaac gtgatgaatc aggcaccggt cgaattattt accgcgcgtt
     2281 ttatcaaaac ggccggcttg ctcagatcga gaaagatacg agaggaaaag gtaagatcga
     2341 tttttggatt cactacgata cgagtcgcgc cgaacaaatt atgatcaaag aagaacaaga
     2401 tatggacgga gacggcattg ccgatctgtg gacgtactat gaggacgggc ggatcgtccg
     2461 acgtgatgtc agcgcggtgg gcttggagct actcgccaag caggaacaga tctcactgcc
     2521 gatcggcgac gtgaaagaga ttcaagtccc tgccaactga tatcatgaac aattcagcca
     2581 ctgtctggga ggattcagtt ttctcactgt tccggagcgt ctttttattg ccctccctgc
     2641 tcctgcacgc gattttccta gctctcgtct tccgtgccgc cagcctgtcg gtgacgaagc
     2701 ccgcattgga cccgccgatc tcggtccagc tggtggaggt aaacgaaggc ggttcctcga
     2761 ataaaagcat cggctcgagc aaaggtccag gcgggccgcg cacctctcca aagctcggca
     2821 atccgatcgc tccggcgcag cgcaccggca aattggacag cggttctctc gatgcctcga
     2881 gggcaagtga tgctgtcgaa gcgacagccg cgccgacacc gatggcgctt ccgggaccta
     2941 aagttctgtc tagcgattcg agtcgtgaag ctgtcaacgt gaaagagact tcaccggatt
     3001 cgctggtgcg gctgccgacg aaagaagccg cgaccaattt gtcgggcgga atggcggtgg
     3061 atccccaaac gagtgagaaa agcttagctg cgctgaaaaa cacgtatccc ggagcaggca
     3121 tcaaggcgct aagggaaggc gaacagatcc ccggttcatt gaaaggcacg ggaactggac
     3181 cgggaccgta cggcgttccg ggtggaagta ggaccggcag tggtttgtcg ggcggcggca
     3241 gtggctctgg cgagggcggc ggcagtcata caggcctaaa gggaatcggc aattctgatt
     3301 acaatcagta cctagagcaa cttaagagaa gagtggaatc cgtgtggaaa tatcccgacg
     3361 acgtcaccgg agtccaaaag gttgccgtgc gtttcagtct tgatcgcgcg ggcaagctca
     3421 ctttggcaga ggtcctggat tcttctgatt ctagactcaa caccagcgcc gtcgaggcga
     3481 ttaagcgagc gtcgcctttt ccacctattc cggagagttt gaaagatctc gccaacgaac
     3541 ctatgatcat caggttcgag gtcgctatcc gcgtccgcgg atgagacttc cgcttcgtcc
     3601 gtcccgaaac cctcgttgaa ttaattatga actaaggtaa tctcaccgga gatttccgaa
     3661 ggaacgcatg atggaacaac aagggctgaa tctcatcgac atcattcaca aaggcgccct
     3721 ggcaacttat ccgctgattc tactttccat cgtaagcgta acggtcgtct tcgaacgttt
     3781 atggtcattg agaaatataa gatcgacgac tttgaggatt gctgaatccc tgattgatcc
     3841 ggttcaaaaa ggtcaacgcg atctggcact ggctatatgc aaacagaatt cgcactgtcc
     3901 ggcgggaaga attttccaca gtgtcttaga gagagccgaa agccaccaac cggaaacagg
     3961 gagcaatgtc gcggttgaag caatgtttga agaaacccag catctgcgaa aacatctttg
     4021 gatcttggga accgttgcca gcagcgcgcc gttcatcggc ctatttggaa ccgtagtcgg
     4081 aatcatcaaa gctttcgaaa gcatggcggt cagtggaacc ggcggcttcg ccgtagtagc
     4141 tgcaggtatt tcggaggcgc tggttgccac ggctttggga ttaggggtag cgattatcgc
     4201 gttggttttt tacaactatt ttcagacccg gattggcagc ttgacggctc tgttccgcat
     4261 ccaagtcgca cgaattcttc gccattttaa cggttgaggc ttccgccatg gcactgactc
     4321 cactcggcaa cgaagagagc tcggatattg tcgctgaaat caatattacg cctctgaccg
     4381 acatctttct cgtcttattg atcattttca tgattaccag ctcggcaatg atcgagtctg
     4441 gcggaaaagt gaatctgccc aaagctgtcg cgactcagtc cgagagccgc ggcacgactg
     4501 tcacccttac tccgaaacgt gagatcttcg ttaatcagaa acagatcact gaggataaat
     4561 tggaagcggc tctcaaggac gcact
//
LOCUS       DASUTG010000208         3406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2109633, whole genome shotgun sequence.
ACCESSION   DASUTG010000208 DASUTG010000000
VERSION     DASUTG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109633"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..543)
                     /locus_tag="VF089_04660"
     CDS             complement(70..543)
                     /locus_tag="VF089_04660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6173284.1"
                     /translation="MPTPIEVIDKMLELARVQKNDVVYDLGSGDGRVVIRAAKKYGAR
                     AVGIEMDQMLLDKARKSAQTEGVSHLVEFRAEDALKTDLSPATVVTLYMLPWFNEAMK
                     PNFRKYLKPGSRIVAHDFGIEGWKPDKTERLPEPEKKAGGYVHYHSIFLWNVGEN"
     gene            806..2134
                     /locus_tag="VF089_04665"
     CDS             806..2134
                     /locus_tag="VF089_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173285.1"
                     /translation="MMQNNKTDTAARPPLSLPFYYGWFVLSIVFMTTLTSAGVRSSPS
                     VLIHPLEAEFGWSRALIASAVSMNLLLFGVAAPISGWLIDKYGPRKVMMGSLTLLIFG
                     VSGTMAMDQFWQFFLVWGVVVGLGAGGVGSVLTATVGNRWFVARRGLALGILGSASST
                     GQIIFLPLFMAAITYAGWRMGSMALIIVAIILVPLIYLFMRDDPSEVGLEPYGAGDPT
                     AASSGGVASLRGMRSGNATITAREVISHPTFWLLASSFFVCGGTANGLIGTHLIPHEI
                     DRGIPQITAASLLGVMGALNMVGTIFSGWMIDRVQPQRWLALVYALRGLSLLMLPFVH
                     SISGFFLFAVIYGLDWFATVPPSMAITADTFGKQNVGKVYGWIFMSHQIGAAIMASSA
                     GALRTWLGDYHSAFFSGGVIALVAAGLALQIKTKPKAVPPVTVHAQTAGA"
     gene            complement(2171..2611)
                     /locus_tag="VF089_04670"
     CDS             complement(2171..2611)
                     /locus_tag="VF089_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173286.1"
                     /translation="MAYGTRWSSRQVKIISTSLYLFALIALLASGCGRDLSTAQGVTE
                     AFVDHHYVSFDLQKAKDYTVSIALEKVNEEIRLTTGQTIDASTQKPRVNYRLLEKKEG
                     DRRASFLYEGTIQSDDGTSFTRKWLIAARRDGDHWRVSNFTESD"
     gene            complement(2575..3342)
                     /locus_tag="VF089_04675"
     CDS             complement(2575..3342)
                     /locus_tag="VF089_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6173287.1"
                     /translation="MLEEKVSFSINGAKTIVGILHHPVGVSPHGAIILCHGMDSTKNS
                     EKLVSLGRSLARRGFLTLRFDFAYVGESSEKFEDITYSGEVEDLRAAFSFMRDRYSGG
                     IGILGSSMGGAVALLFAAEEPGVAALVTIAAPVHPEAFPRRVLSRDALQKWRERGFTF
                     YNGQRLKLALLEDLEMINVVESASKIGCPVLIIHGDNDEIVPVEEALELNACISGKKK
                     LLILRGTDHRLSDPLTMQEAFTEAVDWLTAHVGRRAK"
BASE COUNT          695 a          903 c          961 g          847 t
ORIGIN      
        1 ggggcacact cgcgcaaaac aatgggcgaa gcgccggttc ctgctttctt tgttgcgagc
       61 cgcgagcagt tagttctcac cgacattcca taaaaaaatg ctgtggtagt gaacgtatcc
      121 gccggctttc ttttcaggtt caggtaatct ttccgtctta tcgggtttcc agccttcgat
      181 gccgaagtcg tgagcgacaa tacgggatcc gggtttaagg tatttacgga aattgggctt
      241 catagcttcg ttgaaccacg gaagcatgta aagcgttacg actgttgccg gtgagaggtc
      301 ggtcttcaaa gcatcttctg cacgaaattc gactaagtga ctgacgcctt ccgtctgggc
      361 ggattttcgc gctttatcga ggagcatctg gtccatctcg atgccgacgg cgcgcgcacc
      421 gtacttcttg gcggcgcgga ttacgacccg tccgtcgccg ctgccaagat catagaccac
      481 atcatttttc tggactcgag ccagttccag catcttgtcg atgacttcga tgggtgtcgg
      541 cacaaaaggg atctcctccg gaaattctgc atttgcgtta acaaggcccc aggcgagtag
      601 gcaaacggat ataaccagcg aggataaatt ttctttcatc gcgatcctac aatccaaaaa
      661 ttttggaacc ttgtctcagc gcgacggaca gggttcttgt aagcagctgt ccgtatcgct
      721 acaaagcgtt tagccggatg cttatggggt cagcgccggc agcgacaccg ggtttgtgaa
      781 gtaagctcaa actggtttat ataaaatgat gcagaacaac aagacagata ccgcagcgcg
      841 cccgccgtta tccttgccct tctattatgg ctggttcgtt ttatcgattg tctttatgac
      901 aacgttgacc agcgccggcg tacgttcctc gccgtcggtg ctgattcacc ctttggaagc
      961 cgagtttggc tggagccgag ctttaattgc ctcggccgtc agcatgaatc tattgctgtt
     1021 cggcgttgcc gcgccgatca gcggctggct gatcgacaaa tacggcccgc gcaaagtcat
     1081 gatgggcagc ctgactctat tgatttttgg cgtaagcggc acgatggcga tggatcagtt
     1141 ctggcaattt tttctggtct ggggagtggt cgttgggctt ggcgccggcg gggtgggctc
     1201 ggttttgacg gcgactgttg ggaatcgctg gtttgtcgcg agacgcggcc tggcattggg
     1261 gatccttggt agcgccagct ccaccggtca gatcattttc ctgccgttgt tcatggctgc
     1321 aatcacctat gccggctggc gcatgggatc gatggcattg attattgtcg cgataatctt
     1381 ggtgccgctg atctacttat tcatgcgcga tgacccgtcc gaagtcggac tggagcctta
     1441 tggggcggga gacccgacgg ctgcaagctc gggcggcgtg gcttcgctgc ggggcatgcg
     1501 atctggcaat gccaccatca ccgcgcgcga agtgatatcc catcctacct tttggctatt
     1561 ggcgagtagc ttctttgttt gcggcggaac cgccaacggt ctgatcggca cccatctgat
     1621 tccgcacgag atcgaccgag gtattccgca gataacggcg gcgtctttac tcggggttat
     1681 gggcgcgctc aacatggtcg gcacgatttt ttcgggttgg atgatcgacc gtgtgcagcc
     1741 tcaacgatgg ctggcgttag tctacgcgct ccgggggctc tcgctgctca tgctgccgtt
     1801 tgttcatagt atctcaggat tttttctttt tgcggtaatt tacgggctgg attggttcgc
     1861 cacggtcccg ccgtcgatgg cgatcactgc ggacactttc ggcaagcaaa acgtcggcaa
     1921 ggtttatggc tggattttta tgtcgcatca gatcggcgca gcgattatgg cttcgagcgc
     1981 gggcgcgctt cgcacctggt tgggcgacta tcactctgcg tttttttccg gcggggtgat
     2041 tgcgctggtc gccgcgggat tggccttgca gattaaaacc aagcccaagg ccgtcccgcc
     2101 cgttacagtg cacgcgcaga ctgcaggagc gtgaacagcg atgagcgcag gctgaagacg
     2161 taggcccctg tcagtcagat tcggtgaagt tcgacacacg ccagtgatct ccgtctcgcc
     2221 gggcggctat cagccatttg cgcgtaaaag aggtgccatc gtcggattga atcgtccctt
     2281 cgtaaagaaa agaagctcgc cggtctcctt cctttttctc cagaagtcgg tagttgactc
     2341 gaggcttctg agtcgaggca tcgattgtct ggccggtagt cagcctgatc tcttcattaa
     2401 ccttctccaa agcgatgctg acggtgtaat ctttggcctt ttgcaagtcg aaactcacgt
     2461 agtggtgatc gacgaatgct tcggtgacgc cttgggcggt actgagatcc cgaccgcagc
     2521 cgctggccaa cagagcgata agagcaaaca gatacaaaga tgtggagatg attttcactt
     2581 ggcgcgacga ccaacgtgtg ccgtaagcca atcgacggcc tcggtaaaag cttcctgcat
     2641 agtcagcgga tcggacaggc gatggtcggt tcctctcaat atcaagagtt tctttttacc
     2701 cgagatgcac gcgttcagct caagcgcttc ttcgacgggt acgatctcgt cgttgtcgcc
     2761 atgaatgatc agtaccggac aaccgatctt gctcgcggat tcaacgacat tgatcatttc
     2821 caaatcctca agcaacgcca gcttaaggcg ctgcccattg tagaaagtaa agccccgctc
     2881 gcgccatttt tgcaacgcgt cgcggctgag aacccggcgt gggaaagctt ccgggtggac
     2941 cggcgctgca atcgtaacta gagccgcaac gcctggttcc tcggcagcaa acagcaacgc
     3001 gacggcgccg cccatactgg agccgagaat tccgattccg ccggaataac gatcacgcat
     3061 gaacgagaat gcggcgcgca ggtcctcgac ctcgccgctg tatgtgatgt cttcaaactt
     3121 ttcgctggat tcgccgacat atgcaaaatc aaatctcagc gtcagaaatc cgcgcctagc
     3181 taaactgcgg ccgagggaaa ccagtttttc gctgttcttg gtggaatcca tgccatggca
     3241 caggataatc gctccatgtg gggatacgcc gaccggatga tgcagtatgc caacgatagt
     3301 cttagcaccg ttgatactga aggatacttt ttcttccaac atttggctcg cgcgtaccgg
     3361 ctagcaggac tctagctcag attcatttga gtttgaaggc agtttt
//
LOCUS       DASUTG010000209         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2109698, whole genome shotgun sequence.
ACCESSION   DASUTG010000209 DASUTG010000000
VERSION     DASUTG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109698"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF089_04680"
     CDS             <1..204
                     /locus_tag="VF089_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6173288.1"
                     /translation="QRALQCAYCTPGFVLSVKAMLDENPDPSDEDVREYLSGNLCRCA
                     GYADILRAVRVAQERRKSLATGT"
     gene            415..2304
                     /locus_tag="VF089_04685"
     CDS             415..2304
                     /locus_tag="VF089_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6173289.1"
                     /translation="MQVAQTIKLAAFLPNLHNLDHLFETPQKKTVIQLRWPLVILSCY
                     LLVYSPGDWLYVSQIQAIAVFYLLTNVTLYCVAEDYFNCPKFYGPLLLFDTFFLALTL
                     VLSGGTTPDLVIACLLTLILSCICNDPRGLLVITFLAPAVYGYVVLTGTMTDDPSVYL
                     RLPLPLVISIFYGYFAQVERQKQAAIDKEQQADRELKTAEQVRTQRARLEVLHQTSPA
                     LIGDVGEGKILDVALQMALSHLPYAAAMVTLRNPETGMLQTAASKGFLNRKSDAAVSS
                     LPSLEEVIKNETTLAIKNIYEDPRVQDIGFLHDEGLVAFLGVPLRVNGGVFGSLGFFT
                     RWQYDFTAEEREFAETLAGQLAMAVEQTRLLDRIERQANELRDANKLKDEFLGVASHQ
                     LKSPLNILARNTNMLIERTLGDISPIQEKSLEMMMRQCKELHAVITSMLQVNFIESNL
                     VEPELHETNLWEFLYDLRSSYDSQLTKDIKLIWEFSPDLPLLEADRGKLKLVLQHVID
                     NAIKFTEHGYVRVSAHYLASRKVMEFEVSDTGIGIAADELPMIFEKFSRADSSESAYS
                     GAGLGLYIVKRVVHLLEGNIDVQSRVGRGSTVTIRIPARRRKLPRMQEQLAFAMEPEG
                     SGTGW"
     gene            complement(2329..3153)
                     /locus_tag="VF089_04690"
     CDS             complement(2329..3153)
                     /locus_tag="VF089_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173290.1"
                     /translation="MAKYRARICPNCRFFVGFSVTKMPASDKEAGIISFCLNCSYRLP
                     IHTVINGIRRATAKFGRNRLKLATSSAVVGQVDSGDKTSRGPTSNDRTIPPESYSRYL
                     RTIGQQLQQEGIDSFNLGCGGEGYSVWTRAHTVTWPNRSLFSFSNRLENWWTIRSHGQ
                     DGYQSRAPDGHARFEYSLEDLARMDLAARIHRDPTSGFANGHSLSQLLRTIGSLAYQR
                     NHRLLAISWRDISICAVLENAQGKREIEVFRPDNLYDIWVRMYLRRENRALSDVPQ"
     gene            complement(3463..>4579)
                     /locus_tag="VF089_04695"
     CDS             complement(3463..>4579)
                     /locus_tag="VF089_04695"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX6173291.1"
                     /translation="KEAGRFVAVSAGDGLEATMAGHGSGAVAHSAKTRGVVLNIDVGG
                     GTSKFALCNNGKVQEVTAIDVGARLLAFDQDGAVVRIEEAGRKHARWAGIEVELGQKI
                     SPENLRLMVSGMIEKLFALLKSGPLSDDVKGLLRLPPLSYRGEIDSLMFSGGVSEFIY
                     NREKTSFGDLGPLIAEEIHRRMPELGMLVMEPAARIRATVIGASQYTIQVSGNTIFIS
                     PEDAVPVRNIPVVAPEFDFDGDDVDQKAVVLAVQNALRRLDLLNGRQPVALSFQWRGS
                     ATFARLKAFCSGVAEGLQEILAKRHPLTLINDGDIGGIVGLHFQEELRVENPIISIDG
                     IALSDFDYIDIGALIPTSGSVPVVIKSLIFPASANDH"
BASE COUNT         1040 a         1253 c         1227 g         1059 t
ORIGIN      
        1 cagagagcac tacagtgcgc ctactgcact cccggtttcg ttctatccgt caaagcgatg
       61 ctcgatgaga atccggaccc ttccgatgaa gatgttcgtg aatatctctc tggtaacctg
      121 tgccgctgcg ccgggtacgc cgatattcta cgggccgtcc gcgtcgcgca agagagaaga
      181 aagagccttg ccaccgggac ttaaaccgcg agaagacggc acattccgcc tataataggt
      241 acactgctgc gtcgacaaat ttgtccccat ttgttgtaat ttgtccattt cagatcggtg
      301 ctgttttgaa gccttcgcgc aactcgccga tcttatgtaa caaacggaaa gggcgataca
      361 aaacaattcg ttacggggca tggcatataa tgtgcaatca catggaaaca gcctatgcag
      421 gttgcacaaa cgatcaagct cgctgcgttc ttgccaaatc ttcataatct cgatcatttg
      481 tttgaaaccc cgcagaagaa gactgtcatt caactgcgct ggccgctggt cattctctct
      541 tgctacttgc ttgtctattc gccgggcgat tggctctacg tatcgcagat tcaggcgatt
      601 gcagtctttt atctactcac taatgtaacg ctatattgtg ttgccgagga ttacttcaat
      661 tgcccgaagt tttatggccc gctgctgctg ttcgatacat tctttctcgc gctcacactg
      721 gtgctgagcg gcggcaccac tcctgatctt gtcatcgctt gcctgctcac tttgatccta
      781 agttgcattt gcaacgatcc gcgcggctta ttagtcatca cgtttttggc tccggccgtt
      841 tacggatatg ttgtgcttac cgggactatg accgatgatc cgagtgttta tctgcgcctg
      901 cccttgccgc tcgtgatctc gatcttctat ggttacttcg cccaggtaga gcggcagaaa
      961 caggccgcta tcgataaaga gcagcaagcc gatagggagc ttaagacggc cgaacaagtg
     1021 cgaacacagc gggcgcgcct cgaggtgctt catcagacca gtccagcgct catcggagat
     1081 gttggagaag gcaagattct cgatgtagct ttgcagatgg cgctgagtca ccttccctat
     1141 gcggcggcca tggtcacctt gcgcaatcct gaaaccggca tgctccagac ggcggccagc
     1201 aagggatttc tcaaccgcaa atcagatgcg gcggtctctt cattgccctc tctcgaggaa
     1261 gtcataaaaa acgaaaccac gctagcgata aagaatatct atgaagatcc aagggttcag
     1321 gacatcggct ttttacatga tgagggtttg gttgcttttc tcggggtgcc gttgcgcgtc
     1381 aacggcgggg ttttcggcag cttgggtttt ttcacacgct ggcaatacga tttcactgcg
     1441 gaagaacgtg agttcgccga gacattagcg ggtcagttgg caatggccgt cgagcagact
     1501 cgcttgctcg atcggatcga gcgccaagcc aacgaattac gcgacgccaa caaactcaag
     1561 gacgaatttc taggcgtagc ctcgcatcaa ttaaaaagcc cgctcaacat actggcgaga
     1621 aataccaaca tgctgatcga aaggacgctt ggcgatatca gtcccattca ggagaagtcc
     1681 cttgaaatga tgatgcgtca gtgtaaagag cttcatgccg tcatcaccag catgttgcag
     1741 gtcaacttca tcgaatcgaa tctggtcgag ccggagcttc acgaaaccaa cttgtgggaa
     1801 tttctctatg acctgagatc ctcctatgat tctcagctga ccaaagacat aaaattgatc
     1861 tgggagtttt ctcccgactt gccgctactg gaagccgatc gcggcaaatt gaagttagtt
     1921 ctgcagcacg tcattgataa cgccatcaaa ttcaccgagc acggctacgt cagagtttca
     1981 gcgcactacc tggccagcag aaaggtgatg gagttcgaag tctcggatac aggtatcggg
     2041 attgccgccg acgagctgcc gatgatcttt gaaaagttca gccgggccga tagctcagaa
     2101 tccgcttaca gcggcgccgg gctggggctc tacatcgtca agcgagtcgt gcacctactc
     2161 gagggtaaca tcgatgtgca aagtagagta ggtcggggat cgactgtgac tattcgcatt
     2221 ccggcgcggc gacggaaact gccacgcatg caggagcaat tggcttttgc gatggaaccc
     2281 gaaggttctg gaaccggatg gtgaatcggt atcaatgaag gccagcggtc actgaggaac
     2341 gtccgaaagc gcgcggtttt cgcgccgcaa gtacattctt acccagatgt cgtacaaatt
     2401 atccggtcga aaaacttcaa tctctcgttt tccctgcgcg ttttcgagaa cagcgcagat
     2461 cgatatgtca cgccatgaaa tcgccaacaa gcggtggttt ctctggtaag ccaaagagcc
     2521 gatggtcctg agcaattgcg ataagctgtg tccattggca aatccactcg tcggatcgcg
     2581 gtgaattctc gcggccaagt ccatgcgcgc aagatcttct agagagtatt cgaatcgcgc
     2641 atggccgtct ggcgcgcggc tctgatagcc gtcctgcccg tggctcctga tagtccacca
     2701 gttctccagc ctgttgctaa agctgaaaag ggatcggttc ggccatgtta cagtatgggc
     2761 gcgggtccac actgaataac cctctccgcc acatcccagg ttgaaactat cgattccctc
     2821 ctgctgaagc tgctgtccga tggtgcgcag gtaacggcta tagctttccg gcggaattgt
     2881 gcggtcgttg ctcgtcggac cgcggcttgt cttgtcaccc gagtccacct gtccaacgac
     2941 tgcagagcta gtcgcaagct ttagccggtt tcgcccgaat ttggccgtgg ctcgtctgat
     3001 gccattgatg acagtatgga tcggcaatcg atagctgcag ttcaaacaga aactaatgat
     3061 gccggcttct ttgtcgctgg caggcatttt cgtaactgaa aaccctacga agaagcgaca
     3121 attgggacag atccgcgcgc ggtatttagc catgatcgac gtccattcgc cgcttcatta
     3181 ttcctaacgg ctcgtcatgt caaggaggga tggagctatc tcgatccccg actgagcgac
     3241 tctccggaat agattcaatt tcgcgcttac caaggcctgg cgtggctgaa cgggaactga
     3301 atcttcccct gagaagaggg agagacaaag ccggtgactg gagtgccgcc aaaagatgcg
     3361 gtaaggacga tggaggaaga attggcttgt tgcgtaatct tacttgtgcg cgagcgcccc
     3421 taagctaaac ccgttgtacg gcctgaacga tttgaaacct atttaatgat cgtttgccga
     3481 tgccgggaag atcagcgatt tgatcaccac cggcaccgat cctgatgtgg gaatcagtgc
     3541 gccgatatcg atgtaatcga aatcgctgag cgcaataccg tcgatcgaga tgatcgggtt
     3601 ttctacccgc aactcctcct ggaagtgaag cccgacgatg ccgccaatat cgccgtcatt
     3661 gattaacgtc aacggatggc gctttgccag aatctcctga agtccctcgg cgacgccgct
     3721 gcagaaagct ttcagtcggg cgaaagtcgc cgacccgcgc cattgaaacg ataatgccac
     3781 cggttgtctg ccgttgagca gatccaatcg gcgcaacgca ttttgcacgg ccaagacaac
     3841 ggctttctga tcgacgtcgt cgccgtcgaa gtcgaattca ggcgccacca ctggaatatt
     3901 ccgtaccggc accgcatctt ccggtgaaat gaaaatggta ttgccgctga cctggatcgt
     3961 gtactgtgag gcgccaatca ccgtggcacg aatgcgcgcg gccggttcca tcacgagcat
     4021 gcccaattcc ggcatgcgcc ggtgaatctc ttcggcgatc aacggcccga gatcgccgaa
     4081 gctggttttt tcgcgattgt agataaattc cgaaacgccg ccggaaaaca tcagcgaatc
     4141 gatttcaccg cgataactga gcggcggcag ccgcaggaga cctttgacgt catctgacag
     4201 cgggccggac ttgagcaggg cgaagagctt ttcaatcatc cccgagacca tgagacggag
     4261 attttccggt gagatcttct gaccgagctc aacctcgatc ccggcccagc gcgcgtgctt
     4321 gcggccggct tcctcgatgc gaaccacggc gccgtcctga tcgaaggcga gcaggcgcgc
     4381 gccgacatcg atggcggtga cttcctgaac cttgccgttg ttgcaaagag cgaatttgct
     4441 ggtgccgccg cccacgtcga tattgaggac gacaccgcgc gtcttggccg aatgcgccac
     4501 agctccggac ccgtggccgg ccatggtcgc ttccaggcca tcgccggcgc tcacggcgac
     4561 gaacctgccg gcttcctta
//
LOCUS       DASUTG010000210         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_34073, whole genome shotgun sequence.
ACCESSION   DASUTG010000210 DASUTG010000000
VERSION     DASUTG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34073"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1711)
                     /gene="ggt"
                     /locus_tag="VF089_04700"
                     /pseudo
     CDS             complement(137..1711)
                     /gene="ggt"
                     /locus_tag="VF089_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2244..2597)
                     /locus_tag="VF089_04705"
     CDS             complement(2244..2597)
                     /locus_tag="VF089_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173292.1"
                     /translation="MRPKVRHIAVMTNNREKMVEFYQKIFGLEPKRGYGGAIYMSDGD
                     VNIALIEIKRDDQKKGINHFGFEVDDLDEIKRRLKEAGFPSEIDTKSDKDSDHRIQDP
                     DGHWVDMAVHLRWPK"
     gene            complement(2612..>3644)
                     /locus_tag="VF089_04710"
     CDS             complement(2612..>3644)
                     /locus_tag="VF089_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173293.1"
                     /translation="EQEDGTECWVIDGRRLLLPGVAVAGALMADRAREPQRWREVPPM
                     AFSATERLKAMDCDGVDYSVLYPIVGGLAGESFGKLDDPDLELACVQAYNDWLIDEWA
                     SVSPRFVPQCMVPIWPVERTVAEIRRAVAMGHKGIIYPASPMELRDVPHINEPAYDPL
                     WAVCEELGVPISFHSGASLRIQMRPGDDFSPALAAAFSNVVRPVSSIAVVANFIISRV
                     LQRFPKLKIVFAEGSLGWGAYEMEYADYQSDADGLPSEGYTLRPSELFQRQCYFSCWY
                     DQMSLRVRDYLGTANILWSSNFPLATSTWPNTRRRAELAFAELPEIEKRQIQWENAAK
                     LYSIDATEK"
BASE COUNT          827 a         1072 c          927 g          818 t
ORIGIN      
        1 tcagcgtata tcccctggaa aagctgctgc agtctagcag taaacgatca gtgcgaacgt
       61 cctgccagta gccagtgcgt gaaacaccgt atcgcccgag ccgaatggat cagaaaacct
      121 caagcagttc gattattcag aatcccaagg cgcagccgcc tttgcgcggg tccgagcctc
      181 caacgaaagc gccggattgc agatcgatga gaattgcatg agcgccaccg aagctctccg
      241 gagttccgtc aacgatccgg tggccgcggc gcgacaagaa gtccctaatc ccttgaggga
      301 attgcggctc caacgatact ttctccgcat cgagatgatt gaagcgcgcc gccgaaatcg
      361 cttcctgaac cgtcatgccg aagtcgatca ggttacagat gatctgcacc tgcgcttgcg
      421 gctgcacgtg gccgcctttg agtccgagcg ctgcatacgg tcttccgtcg tgcaagatca
      481 tgccgggcat caatgtatgc acgcaacgct tgtgcgggcc gatgcaattt gcatgattct
      541 catcgagtga aaataagtga ccacggttct gcaggataat cgccgtttca ggttcgacca
      601 cgcccgaacc gaatcccatg aaattgctct gaataaaaga tacgagattg ccttcgcggt
      661 cggcggcggc aacatattcc gtgtcggtgc ccgatggcgc attcaccatt tcagcggcgg
      721 cgcgatcggt tcggattttt tgccgctgca aatcggcgtg gctatcggat atgagatcca
      781 tgaccggcac tttcatgaaa tcacggtcgg cgagatgtcg atcgcggtcg tcaaaggcga
      841 gcttcttagc ctcaatcatc agatgcaagg catggccact gtttccgccc aatgatttta
      901 gatcaaatcc ttcaaggatc ttcagcatct ccagtgcgac aaagccttga gttgccggag
      961 ggagctcgta gatatcgtag ccgcgataac tcgcggaaat cggctccacc caatccgaac
     1021 gatgggcggc gaaatcttcg acggtgaaca gcccgccgtt cttttaagag cagcgaacga
     1081 tcttcgccgc gatatcgccc cggtagaaaa actccgcgcc gccttcggcg atccctcgca
     1141 acgtctttgc catgcggggg ttcctaaaga tctcgcctgc cttgggggcg cgatcgttca
     1201 ccagataatt gagagccgct ccttcatcag ccttcagcct ctcctccgac tcatgccacg
     1261 actccgccgt tagctcggca accggaaacc cctcatccgc atattgaatc gccgcctgca
     1321 agacttcacc gagcgttcgt gtgccatagg cgtttgataa cgtcacccat ccatccaccg
     1381 cgccgggaac cgttacagaa tgtattcccc gctgcggcat ttccttgaag ccgctcttgc
     1441 ggcaaaagtc gagattggct gcgaaaggcg acctgccact ggcatccaag gctttaaggg
     1501 tttttcctcc agcagaataa aagaccgcaa acgcatcgcc gccaatgccg attgacatcg
     1561 gctcgacgac accgagcacc gccgccgtcg cgatggctgc gtcgatggcg ttgccgccgt
     1621 tcataagaat ctgcacgcct gccaaagtcg caagcggttg gctggatacc accatgccgt
     1681 ttttcgccaa cactgaaaag atttccgcca tgatttcctt ctgcaggtta ggaagcaata
     1741 aaggatattt tcatcaggcc gcttaaagat ctcacatgag agagccgcga tccacccgga
     1801 gccaaagcgt attctttagt acgtcgcaac gagacaatga tgcatagctg cgcggcaaga
     1861 tttcacgctt tgacccggca atacaatctc cctggttgcc atgcaccacg tgaacagatt
     1921 cttaccggcc ttactctcca ggaccgtttg gttaagcaaa gcaccgccgc tttgggatcg
     1981 aagctttctt gcgagtgaaa caaagccggg caacatcatc atcgcgaggt ccagcaagtg
     2041 aagtctcaga ttcacaaagc tgtagccggc atggaaacta atggacgatg cagtgccagt
     2101 cttaatagag acaactaaat aggtgagctt aggctcctgt tgttccgggc gaaggaggaa
     2161 cttgcgcttc ttgtttacga tctgacccga gatactcgca ggcccctgac actcagtacc
     2221 agtcatcttc gttttacctg cctctacttg ggccagcgga gatgaactgc catatcgacc
     2281 cagtgtccat caggatcctg gattcggtga tcggaatctt tatccgactt ggtgtcgatc
     2341 tcggagggaa agcccgcctc cttcagcctg cgtttgatct cgtcgagatc gtccacctcg
     2401 aagccaaaat ggttgatccc ctttttttgg tcatcgcgtt ttatttctat aagcgcgatg
     2461 ttgacgtccc cgtccgacat ataaatggcc ccaccgtagc cgcgcttggg ctcgagacca
     2521 aaaatctttt gatagaactc caccatcttt tcacgattat ttgtcatgac tgctatatgt
     2581 ctcaccttgg gccgcatgtt tttgcctcct ttcatttttc ggtagcgtct atcgaataga
     2641 gttttgctgc gttctcccat tgtatctgtc gtttctctat ctcaggtaat tcggcgaaag
     2701 ctaactccgc tcgacggcgc gtgttgggcc atgtggaagt tgccagggga aaattgcttg
     2761 accatagaat attcgcggtg ccgagataat ctcgaacgcg taggctcatt tgatcgtacc
     2821 agcagctaaa ataacattga cgctggaaca gctccgaagg cctgagcgta tagccttccg
     2881 atggtaagcc gtcagcatcg gattggtagt cggcgtactc catttcatag gctccccagc
     2941 caagcgagcc ttcagcgaag acgatcttca gcttgggaaa tcgctgcagc acgcgcgaaa
     3001 taataaagtt tgcgaccacg gctatagagc tcaccggccg aaccacattg ctgaaagccg
     3061 ccgccaacgc gggtgagaaa tcatctccgg ggcgcatctg aattctcagg gacgctcctg
     3121 aatggaagct aataggaacg ccgagttcct cacaaacggc ccaaagcggg tcgtatgccg
     3181 gctcgttgat gtgcggcacg tcgcgcagct ccatcggcga tgccggatag atgataccct
     3241 tgtgtcccat tgccacggct cgtctaatct ccgcgaccgt ccgctcgact ggccaaatcg
     3301 gcaccataca ctgcggcacg aagcgcgggc tcacgctggc ccattcatct attagccagt
     3361 cgttgtaggc ctgcacgcaa gccagttcca aatcaggatc atcgagcttg ccgaagctct
     3421 cgccagccaa accgccgaca atcggataaa gcaccgagta gtcgacgccg tcgcaatcca
     3481 ttgccttgag gcgttccgtg gcgctaaaag ccatcggcgg aacctctctc cagcgctgcg
     3541 gttcgcgtgc ccggtcagcc atcagcgcac cagccaccgc gacaccaggc agcaagaggc
     3601 gtcggccatc aataacccag cactcggtgc catcttcctg ctct
//
LOCUS       DASUTG010000211         8450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_478029, whole genome shotgun sequence.
ACCESSION   DASUTG010000211 DASUTG010000000
VERSION     DASUTG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478029"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF089_04715"
     CDS             <1..209
                     /locus_tag="VF089_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173294.1"
                     /translation="RQSYLYVLDTFAREPFVPESVMQAMVQRMVEVNMIDLKSAQATP
                     ISAYFDNSYVAELKQSGFLDNIWK"
     gene            478..1359
                     /locus_tag="VF089_04720"
     CDS             478..1359
                     /locus_tag="VF089_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6173295.1"
                     /translation="MSEYKEILYTKQRGGVLITLNRPEALNAITRPMLKELHQALDEA
                     EADPDVRAIVITGAGRAFSSGFDQTTTTGRRRDIQWPYGIQTGMSAADLMNYWSADDV
                     KLIRIFEMTKPVIGAVRGWAMGGGCWLALYTHITIAAEDAVFAQPEVRHGSNTTFMWT
                     LLAGFKNALRYGLVGDHIDAQEALRIGLVNKVVPVDQLLEESFKIVERIAHVPPETVK
                     INLQISTMGLQMMGLRDALRMDETLSIPAHMMLNEEFRRPLDEARASQGTKAYLQLRD
                     GPFQPEPFGPRSRKKTE"
     gene            1422..3599
                     /locus_tag="VF089_04725"
     CDS             1422..3599
                     /locus_tag="VF089_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6173296.1"
                     /translation="MTDSTNTGTGVAWRNLSPLMNPRSVAIIGASQRSASALNREPRG
                     NRVIRNLKSFGYPGRIVAVNPKYGEVMGYACYSEIGAIPEPVDCVVLAVPNRQVPDLL
                     ESAAASGVRAAVVFSAGFAETGTEGKQRQARLEALSRERGFLICGPNCYGVLNVFGKA
                     PLFASTIPQGFLAGPVALVSQSGGLSTTIANALMLNRHVGLSHIVSCGNQAGATVEEY
                     FNYLVEDENTRVIAGFVEGFKQPHLLLAVARKAAERNKPLIILKGGRSEVSQRAAATH
                     SGSLAGAAEVVDAAFRQSGVVSVRSINELIDTISVFSCDGFVQRYRGGRRIGVLSGSG
                     GECTLVSDAAASVGLEVPELTESTKLHLQEAVADFGNMNNPLDGTGAMYDDEKIFPRL
                     LQALIDDANIDVVTINLEANDPRPKELKSGNRFAQAIETAAAASNKPIACFSSVVGGP
                     VDPEILLPLRQAGVPLMEGAECATTTIRNLAEFYEFQQSRQSEATGDQPAPGRKLASG
                     MLPAQAAFQLLEEFGISAVPMALGQSPEEAAAAAERIGFPVALKIESADITHKSDVGG
                     VALKLSDTAGVRDAYTKMMSEVIRRAPLAKIDGVVVQRMASEGVEMILGIKRDPLFGP
                     VVLCGFGGILVELLKDVSIAIPPLSRQQAYDMLKRLRGFAILAGARGKPAADTDALCD
                     AIAGVSRLAWSFGDQLAGLDINPLIVLPKGEGIVAVDVLVQVL"
     gene            3610..4776
                     /locus_tag="VF089_04730"
     CDS             3610..4776
                     /locus_tag="VF089_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173297.1"
                     /translation="MAMIQETFLGIIVFARPSFRRKPESRLNDFAWMPARAQLGRDDA
                     AGICASLIRSAAELAGALKPLVSLALVLAFALISQRSVFAQAKPLKEVRVPYALGGST
                     GFFWVAQRSGSFEKYGLKVLPIFMRGGREAVQALISRDVTMLQQGSSGVILAWAQGAK
                     DLVVIGATGNKLDYVLVSSAAIKKPADLKGKKIAISQLGAGTDFIARHAVRQLGLNEK
                     DVTLLSIGAQGERWAALAGGHVDASVFQPPITLRARKAGLPIWVDFSKSDYEFVVAGP
                     VTTRSFIKTDRETVMNFMRGLADGMDFYRDEKNKDAVIKYLGEFYKSTNIEELEETRQ
                     VYRQVTPGLPVVTLKSMENMIASDRMLSTMNIKGADVMDLSFLRQLEEERKTRK"
     gene            4888..5769
                     /locus_tag="VF089_04735"
     CDS             4888..5769
                     /locus_tag="VF089_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6173298.1"
                     /translation="MSDYKEILYEKRRGGALITLNRPEAMNAISRSMIKEIHQALDDV
                     EEDKDVRAVVLTGAGRAFSAGMDQGNRAGRRRDIHWPYGILTGQSAAEVIDSWRVDPR
                     NFRRMWEFGKPIIGAINGWAMGAGSWLSLFTHITIAAENAVFAQPEVRHGSNTSFMWT
                     LLGGYKNALRYGLTGDHIDAQEALRIGLVVKVVPPDQLIDECFNLVERIAHVPPETVK
                     INLQVATMGLEMMGLRDALTFDSQMSAPAHIMLREELRKPLDEARANKGIREYLQMRD
                     GPFQPEPFGPKSKKQPE"
     gene            5857..6735
                     /locus_tag="VF089_04740"
     CDS             5857..6735
                     /locus_tag="VF089_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6173299.1"
                     /translation="MTNYKNILYQKQRKGVLITLNRPNALNAMNEELMNEFDQALSEA
                     ETDPEIRAVVITGADGAFSVGEDISGDDPETAWPYGIPVGTSLNATYNKFRDADRKDI
                     LGRQAYRWQYPKPIIAGVSGWCLGAGSWLALTCHITIAADDAVFGQPQVRHGANTDFI
                     WVALAGFKNALRYSLTGDHVDAKEALRIGLVNQVVAKDQLLETCFQFVERVAHVPPET
                     VKINLHISTLGMEMMGLRKAWTLNAELAAMARLTKREEFNKRLEDAKKNGGLKAFLEE
                     RDRPFQPEPFGPHGKK"
     gene            7375..>8450
                     /locus_tag="VF089_04745"
     CDS             7375..>8450
                     /locus_tag="VF089_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6173300.1"
                     /translation="MLGGHAVAQCVRNEGVRHLFCVPGESHIAVLDGLVNTPDIKLIT
                     NRQEGCAAFMAEGYAKSTRKVGVCLVTRGPGATNASIAIHSAKYDSAPLVLLVGQVGR
                     NARGREAGQELDYGQFFGSIAKWVVEINNPTHVPRVITRAFHIARSGRPGPVVVSLPR
                     DMLEEKAEIPMVNPYPSAQPSPDPRLIEEMVARISAAKKPMLLVGSGTQYAGAWQELI
                     DFSEKFQIPVLTSYKRQDAFPNSHANYIGNLSSANKNTRALASSDADLLVVLGCRLSQ
                     QTTGGFSFPRPGQAFIQIDADEQNIGQNARPELAVVADAKLALSAALKQTAPAANAGR
                     TSWIAELHSAQKQFCTPKDRPSRRV"
BASE COUNT         1951 a         2344 c         2427 g         1728 t
ORIGIN      
        1 tacgccagag ctatctttac gtcctcgata ctttcgctcg cgagcccttc gtccctgaaa
       61 gcgtgatgca ggcaatggtg cagcgcatgg tcgaggtcaa tatgatcgat ttgaaatccg
      121 cccaagcgac gccgatcagc gcctacttcg acaatagtta tgtagccgag ctaaagcaaa
      181 gcggtttcct cgacaatatc tggaaataaa ttctgtcatt gcttgacggc gaatcgctat
      241 ttcacgtagt ttgcgaacag ttatcggaag gcttcttaaa acttgttggc tgaagaaatg
      301 ctgcaacgag gtgtcgactg cggctgtcga aagcgatcga gacgaggtcg gaattccctg
      361 gaagagccgg aacccaaaag gaatggatcc aaaactgttc atcgcgagag tctggtacgc
      421 gaagtcgagg ccagtggctt tatcaaggcg ctgtaccaag actgaaaagg agccggcatg
      481 tcggaataca aagaaattct ctataccaaa caacgcggcg gcgtgctcat cacattgaat
      541 cgccccgaag cgctcaatgc catcacccgg ccgatgttaa aagaactgca tcaggcgctt
      601 gatgaagccg aagccgatcc cgacgtccgc gccatcgtca tcaccggcgc cggacgggct
      661 ttcagctccg gtttcgatca aaccaccact acaggccgac gccgggacat tcagtggccc
      721 tacggcattc agaccggtat gagtgccgcg gatctgatga actactggag tgccgacgac
      781 gtcaagctca tccgcatatt cgaaatgacc aaaccggtga tcggcgcggt cagaggttgg
      841 gccatggggg gaggctgctg gctagcgctc tatacccata tcaccatcgc tgcggaggac
      901 gcggttttcg cccagccgga agtgcgacac ggctccaata cgaccttcat gtggacgctt
      961 ttggcggggt tcaagaacgc cctacgctat ggattggtcg gcgatcatat cgacgctcaa
     1021 gaagcgctgc gcatcggcct ggtaaacaaa gtcgtgccgg tcgatcaact gctggaggaa
     1081 agtttcaaga tcgttgagcg catcgctcat gtgccgccag agacagtgaa gatcaatctg
     1141 caaatttcca cgatggggct gcagatgatg gggctgcgcg acgcgctgcg tatggatgag
     1201 actctgtcga ttccagccca tatgatgctc aacgaggagt ttcgccgccc gctcgacgaa
     1261 gctcgcgcca gccaagggac aaaagcttac ttgcaattgc gcgatggacc gttccagccg
     1321 gaaccgttcg gaccgcgatc gagaaaaaag accgagtagc ggagtgttgg tgtaaaggag
     1381 tattggggtt ccggcgcatc actccaattt gaaatgcaac tatgactgat tcgacgaata
     1441 ccggcaccgg cgtcgcctgg cgcaatctct cgccgctcat gaatccgcgt tcggtggcaa
     1501 tcatcggcgc ctcgcagcgt agcgcatcgg cactcaatcg cgaacccaga ggcaaccggg
     1561 tgatccgcaa tttgaaaagt ttcggctacc cgggccgaat cgttgccgtc aatcccaagt
     1621 acggtgaggt gatgggttat gcgtgctatt ccgagatcgg cgcgattcca gaacctgtcg
     1681 attgcgtcgt cttggctgtt cccaaccgcc aggttcctga tcttttggaa tcggcggcag
     1741 cttcgggggt gcgcgcggcg gtggtcttct ccgccgggtt cgccgagacc gggacagaag
     1801 gaaaacaacg ccaggcaaga ctcgaagcgc tctcgcgcga gcgtggcttt ctgatctgcg
     1861 gtccgaactg ctacggcgtt ttgaatgtgt tcggtaaagc gccgctcttc gcttcgacca
     1921 tcccgcaagg atttctcgcg ggacccgttg ccctagtttc acagagcggc ggcctaagca
     1981 cgactatcgc caacgcctta atgttaaatc gccatgtcgg cctaagccat atcgtctcgt
     2041 gcggcaatca ggcaggcgcc acggtggagg aatattttaa ctatctggtc gaagacgaaa
     2101 acactcgcgt gattgccggg ttcgtcgagg ggttcaagca accgcacctg ttattggctg
     2161 tggcccgcaa agccgccgaa cggaacaagc cgttgattat tctaaagggg ggcagatcgg
     2221 aagtctcgca gcgcgcggcg gcaacccatt cgggatcgct ggccggagcg gcagaagtgg
     2281 tggatgcggc atttcgtcaa agcggcgttg tctcggtccg cagcataaat gagttgatcg
     2341 ataccatcag cgtcttttcc tgcgatgggt tcgttcaacg ctaccgcggt ggcaggcgca
     2401 taggcgttct cagcggctcg ggtggggaat gcacattggt ctccgatgca gccgcaagcg
     2461 tcggcctcga agtacccgaa ctgacggaat cgacgaagtt gcatttgcaa gaagcggttg
     2521 ccgatttcgg caatatgaac aaccctctcg atggcacggg cgccatgtac gacgatgaaa
     2581 agatctttcc ccggctgctg caggcgctga tcgatgatgc caacatcgat gtcgtcacca
     2641 tcaacctcga agccaatgat ccacggccaa aagaattaaa gagcggcaac cgattcgccc
     2701 aggcaatcga gacagccgca gccgcgagca acaaacctat tgcatgtttc agttcggttg
     2761 tcggcggacc ggtcgacccc gagattctgt tgccgctaag gcaagccggc gtaccgctca
     2821 tggaaggcgc ggaatgcgca acgaccacca tccgcaatct agcggagttt tatgaattcc
     2881 aacaaagccg gcaatcggaa gcgaccggag accagccggc tcccggtcga aaactcgctt
     2941 ccggaatgct gccggcgcaa gccgctttcc agttgctcga ggaattcggc atttcagcgg
     3001 ttccgatggc gctgggtcag agcccagaag aggccgctgc ggccgccgag cgcatcggct
     3061 ttccggtagc gctgaagatc gaatcggcgg atatcacgca caagagcgat gtcggcggag
     3121 tagcgctgaa gctctccgac acggctggag tacgcgatgc ctataccaaa atgatgagcg
     3181 aggtcatccg ccgcgcgcct cttgcaaaga tcgacggcgt tgtcgtgcag cggatggcga
     3241 gcgaaggcgt cgagatgatc ctcggcatca agcgcgatcc ccttttcggc cccgtcgtgc
     3301 tctgcgggtt cggcggcatt ctcgtcgaac ttcttaaaga tgtcagcatc gccatcccac
     3361 cgctgtcccg tcaacaggcg tatgacatgc tcaagcgcct gcgtggcttt gcaattctcg
     3421 ccggcgcacg cggcaagccg gctgcggata ctgatgcgct gtgtgatgcg atcgcgggtg
     3481 tgtccagatt ggcctggagc ttcggtgacc agctcgccgg cctggatatc aatccgttga
     3541 ttgtgctgcc aaagggggaa ggcatcgttg ccgttgacgt gctggtgcaa gttctatgag
     3601 ggtgaagaca tggctatgat tcaagaaact tttctcggaa tcatcgtttt tgctcgtccg
     3661 tcattccggc gaaagccgga atcgaggtta aatgacttcg cctggatgcc ggctcgcgct
     3721 cagcttggcc gcgatgacgc tgcaggaatt tgtgcgagtc tgattcgcag cgccgccgaa
     3781 ttggcagggg cattgaaacc tctcgtttca cttgcattag ttctcgcatt tgcgctgatt
     3841 tctcagcgct cagtatttgc tcaagcaaag ccgctcaaag aagttcgcgt gccgtatgct
     3901 ctcggcggct ccaccggatt cttctgggtg gcgcagcgct ccggctcctt tgaaaaatac
     3961 gggctcaagg tcctgcccat tttcatgcgc ggcggccgcg aggcggtgca ggcgctcatc
     4021 tcacgagatg tcacgatgct ccagcaaggg agttccggtg tgattctcgc ctgggcacag
     4081 ggcgccaaag acctcgtagt gatcggggcc accggcaaca aactagatta cgtcttagtc
     4141 agcagcgcgg ccatcaagaa gcccgccgat ctcaaaggaa aaaaaatcgc catcagccag
     4201 ttgggcgccg gcaccgattt catcgcccgt cacgcggtgc gccagctcgg gttaaacgag
     4261 aaggacgtca cgctcctcag catcggcgca cagggggaaa gatgggcggc gttagccggg
     4321 ggccatgttg acgcttcggt ttttcaaccg ccgatcacct tgcgcgcgcg caaagcaggc
     4381 ttgcccatct gggtagactt ttccaagagt gattatgaat tcgttgtcgc cggtccggtc
     4441 acgacgcgct ccttcatcaa aacggatcgc gagacggtga tgaatttcat gcgcggcctg
     4501 gcggatggta tggacttcta ccgggacgag aaaaacaagg atgctgtcat caaatacctt
     4561 ggcgaattct acaaatcgac aaacatcgaa gaactggaag agacccggca ggtttatagg
     4621 caagtcactc cggggctgcc agttgttacc ctaaaatcga tggaaaacat gatcgccagc
     4681 gaccgcatgt tatcgaccat gaacatcaag ggcgccgatg tgatggatct gtctttcttg
     4741 agacagctgg aagaggaacg gaaaacgaga aaatgatcgt tcaagcagtt caagccgttc
     4801 aaaaagttta aatcgttatt cggaccgatc cgacggcttg aacgtttgaa ctatttgaac
     4861 gatttgaacc agccgatagg agttcgaatg tccgactata aagaaatact ttacgagaaa
     4921 cgccgcggcg gcgccttaat cacgctcaac cgtcccgagg cgatgaacgc gatcagccgc
     4981 agcatgatca aggaaatcca tcaagcgctc gatgacgtcg aagaagacaa ggatgttcgc
     5041 gccgtcgttc tgaccggagc cggacgggcc ttttccgccg gcatggacca ggggaatcgc
     5101 gccggccgac ggcgggatat tcactggccc tatggcattc tcaccggcca aagcgccgcc
     5161 gaagtcattg attcctggcg ggtcgacccg cgcaattttc gccgcatgtg ggaattcggt
     5221 aaaccgatta tcggcgcaat caacggctgg gccatgggcg caggttcatg gttatcactg
     5281 tttacgcaca ttacgatcgc ggcggagaat gccgttttcg ctcaaccgga agtgcgccac
     5341 ggctccaaca ccagcttcat gtggacactg ctgggcgggt ataaaaacgc gctgcgctac
     5401 ggactcaccg gtgatcatat cgacgcgcag gaggccctgc gtatcggcct agtcgtgaaa
     5461 gtcgtgccgc cggatcagct aatcgatgaa tgttttaacc tggtcgaacg cattgcgcac
     5521 gtaccgccgg aaaccgtcaa gatcaatctc caggttgcta ctatgggact ggagatgatg
     5581 ggactgcgcg atgcattgac ttttgacagc caaatgtcgg cgccggcgca tatcatgctg
     5641 cgcgaggagt tgcgcaagcc ccttgacgag gcacgggcga acaaaggcat ccgggagtat
     5701 ttgcaaatgc gcgacggacc attccagccc gagccgtttg ggcccaagtc gaagaagcag
     5761 ccggaataat ggagtattgg agtaacgggc gggcctcacc caatattcca gcactccagc
     5821 actcgagtgc tcaatctcaa atcacaggag cgagagatga ctaattacaa gaacattctt
     5881 tatcaaaagc agcgtaaagg tgtcctgatc acgctcaacc gtcccaatgc tttaaacgcg
     5941 atgaacgaag aattgatgaa tgagtttgat caggcattat ccgaagccga gaccgatcca
     6001 gagatcagag ccgtcgtcat caccggcgcg gacggcgcgt tttcggtggg cgaagatata
     6061 agcggcgacg atccggaaac ggcgtggcct tacgggatac cggtcggaac ctcgctcaat
     6121 gcaacctaca acaaatttcg tgatgcggat cgcaaggaca ttctcggccg gcaagcgtat
     6181 cgctggcaat atccaaagcc gattatcgcc ggggtgagcg gctggtgcct gggcgccggc
     6241 tcttggctgg cgctcacatg ccacatcacc atcgccgccg acgacgcggt gtttggccag
     6301 ccgcaggtga ggcacggcgc gaacaccgac tttatttggg ttgctcttgc cggattcaag
     6361 aatgcgctgc gctattcact caccggggat catgtcgatg ccaaagaggc tttgcgcatc
     6421 ggtctcgtca atcaggtggt ggcgaaagat cagctgctcg agacgtgctt tcaatttgtc
     6481 gaacgcgtcg cccatgtgcc gcccgagact gtcaaaatca atctgcacat ttcaactttg
     6541 ggcatggaga tgatgggctt gcgcaaagca tggacgctca atgccgagct cgccgcgatg
     6601 gcgcgcctga ccaagcgcga agaattcaac aagcggctcg aagacgcaaa gaaaaacggc
     6661 gggcttaaag cgttcttgga ggaacgcgac cgaccgtttc agccggagcc gtttggccca
     6721 catggaaaga agtagaagcc tgcaccgttg attggcccat tctgtcagaa gttttggtta
     6781 tataggatcg tgtacaataa ccggccgatg taaataccga agtacaagat acctccacaa
     6841 gcggctacgt tgaataaggc attgaggata cggatgaaga agttcatagg tttgcgcatc
     6901 aagttgtaaa tagaatagtc agggattcct ctcttggatc acttagctgt gaggagtccg
     6961 acgggaaatg gaatctaaca atcgtcacat catccaagtc ctactacttc gcagttggcg
     7021 ggcaaagcac gttccatgat gttccctccc gaagttgagc tgacggacct atatcggcac
     7081 ttagcggggc gagtcaagcc aactggatcc acaagcctgt tttcgaccca gcgccgcagc
     7141 tgcagctgac cgcgcgctat atttcgacca ctcttcaggc cgcaccacct cgaccacaag
     7201 tccggtaaat gtctatgcac ccacgcagga tagttgccga ataaaatcga tgtattaaag
     7261 gtattgaagc gttgacagct aagatcggtt cggactatac ggcagttgac gattgcacca
     7321 gcaaacaccg gcacaggatc gattttcgag tgaacttcga ctgaggagac aattatgctt
     7381 ggcggtcatg cggtagcaca gtgcgtgcgt aacgaaggcg tgcggcatct tttctgtgtg
     7441 ccgggtgaaa gccatatcgc ggtcctcgac gggttggtca atacacccga tatcaaatta
     7501 atcaccaacc gccaggaggg ctgcgccgct tttatggctg agggttacgc caaaagcacg
     7561 cgcaaggttg gcgtctgcct ggtcacacgt ggccccggcg caaccaacgc gagcatcgcg
     7621 attcattcgg ccaaatacga ttccgcgccg ctggtgcttc tggtcggaca agtcgggcgc
     7681 aacgcacgcg gccgcgaagc cggccaagag ctcgactacg gccaattttt cggcagcatc
     7741 gccaagtggg tcgttgagat caacaacccg actcacgtgc cccgggtgat aacccgcgcc
     7801 tttcacatcg cgcgcagcgg ccgccccggc ccggtcgttg tatcgttgcc acgcgatatg
     7861 cttgaagaga aagccgaaat accgatggta aatccttatc cgtcggcgca gccaagtccg
     7921 gatccgcgct tgatcgagga aatggtcgcg cgcatcagcg cggctaaaaa acccatgctg
     7981 ttggtgggct ccggcacgca atatgccggc gcctggcagg agctaatcga cttttctgaa
     8041 aagtttcaga tcccggtctt gacctcctac aaacgccagg atgcttttcc gaacagccat
     8101 gctaattaca tcggcaatct atcgagcgcc aataagaaca cgcgcgccct ggcgtcgagc
     8161 gatgccgact tgctggtcgt gctcggttgt cgtctgagcc aacaaaccac cggcgggttt
     8221 tcttttcccc ggcccggcca agcgtttatt cagatcgacg cggacgagca gaacatcggc
     8281 caaaatgcac gcccggaact cgccgttgtc gcagatgcca agctcgcatt atcggcggcg
     8341 ttgaagcaaa ccgcaccggc agcgaatgcc ggccgcactt cctggatcgc cgaactgcac
     8401 agcgcgcaga aacagttctg cacacccaaa gacagaccaa gccggcgggt
//
LOCUS       DASUTG010000212         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_182585, whole genome shotgun sequence.
ACCESSION   DASUTG010000212 DASUTG010000000
VERSION     DASUTG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182585"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF089_04750"
     CDS             <1..638
                     /locus_tag="VF089_04750"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX6173301.1"
                     /translation="VVGIDLVKAQIEIAAGGYLPWRQRHITQTGHAIECRIYAEDAEN
                     DFMPCPGKIEGLRLPEGLGLRNDCGVYEGAEVPINYDPMIAKLITWGENRVEAILRMR
                     RALREYQIRGIKTNISFHQWILRHPHFMSGKVHTGFIDQEYHSTSKEEVFPHKEIALA
                     SAAIAAMHREQESTLGLLAKGATEPSKWRERGKRTALRLQPAFRSRGWRRS"
     gene            649..1152
                     /locus_tag="VF089_04755"
     CDS             649..1152
                     /locus_tag="VF089_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX6173302.1"
                     /translation="MAFIAKLGEQTYTIDIDEVGKSVYKVSIDGNEFLVDGKKTGRTN
                     YSLIVDNRSFEIEVDHTEDEYRILVDGRNYHVNLVDERRVRVGGTQSGIEFQGRQKIS
                     VPMPGKVIAVLVAEGDTVEKGQGLVIVEAMKMENEVRSPINGEVKEIKVTPGETVEGG
                     AVLLIVE"
     gene            1225..1776
                     /locus_tag="VF089_04760"
     CDS             1225..1776
                     /locus_tag="VF089_04760"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX6173303.1"
                     /translation="MIPKKCFFTKGVGVHKEKLASFELALRSAGLAYCNLVLVSSIYP
                     PFCKRISKEEGVKLLRPGEIVFCVYDRESTNEPNRLVAASVGVAIPADQEQHGYLSEH
                     HSFGETEEKAGDYAEDLAASMLATTLGIEFDPDTAWDERENLFKMSGKIVRTSNITQS
                     AIGNKDGLWTTVFAACAFVNDDN"
     gene            complement(1845..2639)
                     /gene="tatC"
                     /locus_tag="VF089_04765"
     CDS             complement(1845..2639)
                     /gene="tatC"
                     /locus_tag="VF089_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6173304.1"
                     /translation="MFRWHPPRLLTNHVSDASAKRHLDNVQMPFSSHLEELRSRLIRS
                     VLAVAIAFVFCFYFADHIFFALSEPLRRLRIPDLTMIGTAVTEAFFTKMKIAFFAALI
                     VALPVLLWQGWQFVGPGLYEHEKRYTRSFVLFGSFFFLAGVAFCYGIVLHKGLALLLK
                     RYQVLQIQPAIQIGDYLSMVARLVLAFGLMFQLPVLSFFLTRVGLVDHRLLIHYFGYA
                     VIGMAIISAVLTPPDLISQILLMLPLCLLYGVSIAVAYAARPRSTQ"
     gene            complement(2593..2868)
                     /gene="tatA"
                     /locus_tag="VF089_04770"
     CDS             complement(2593..2868)
                     /gene="tatA"
                     /locus_tag="VF089_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6173305.1"
                     /translation="MFGIGMPELLLILALALIVLGPKKLPELARALGKGMAEFRRATD
                     ELKDEFRQLENEPPEQSAQAAQKDDPLLTKQADVSVASATPPNEPRE"
BASE COUNT          836 a          787 c          869 g          696 t
ORIGIN      
        1 gagtggttgg gatcgaccta gtcaaagcgc agatcgagat agcggccggc ggataccttc
       61 cgtggcgaca gcgtcatatc acccaaacgg gtcacgctat cgaatgccgg atctatgcgg
      121 aggacgctga aaacgacttt atgccttgcc cgggaaaaat cgaaggtttg cgcttgcccg
      181 aaggacttgg cttacggaac gactgcggcg tttacgaagg tgccgaggtg ccgattaatt
      241 atgatccgat gatcgcaaag ctgattactt ggggtgaaaa tcgggtcgaa gcaatcctgc
      301 gcatgcgccg agcgctgcgt gagtatcaaa ttcgcggcat taaaacgaac atatcgttcc
      361 atcagtggat tctgcgtcat cctcatttca tgtccggcaa agtccatacc ggctttatcg
      421 atcaagaata tcattctacc agcaaagagg aggtctttcc gcacaaggag attgcgttgg
      481 catcggcggc catcgctgcc atgcatcgtg aacaggaaag cacgcttggg ctcctagcca
      541 aaggcgctac cgaaccgtcc aagtggcggg agcgcggcaa gcgcactgcg ctgcgtctac
      601 aacccgcgtt tcgctcgcgg ggttggcgcc gatcgtagcc agagcactat ggccttcatt
      661 gccaagctgg gagaacagac ctacacgatc gatatcgacg aggtcggcaa gtctgtttac
      721 aaggtctcca tcgatggcaa cgagttcttg gtcgatggga agaaaaccgg gcgaacgaat
      781 tactcgttga tcgtcgacaa tcgatcgttt gagatcgaag tcgatcacac ggaagatgaa
      841 tatcgaatat tagtagacgg tcggaactat catgtaaatt tggtggatga gcggcgggtt
      901 cgtgttggcg gaacccaatc aggaatcgaa tttcaaggac gacagaagat ttcagtgccg
      961 atgccgggca aggtgattgc cgtgctggtt gccgaaggcg ataccgtcga aaaagggcag
     1021 ggactggtca tcgtcgaagc aatgaagatg gagaacgagg tgcgaagccc catcaatggc
     1081 gaggtgaaag agattaaagt gacgcccggc gaaaccgtcg agggaggcgc agtcttactc
     1141 attgtagaat gaacatggtg acgcgaaata atttgtcacg gcgaagttgt cagcaatttt
     1201 ctttaatgtt atagacaccg gacaatgatc ccgaaaaagt gtttttttac aaaaggtgtt
     1261 ggcgtccaca aggaaaagtt ggcgtctttt gagttagcgt tgcgatcagc gggattagcc
     1321 tattgcaact tggttctggt atcgagcatc tatccgccat tttgtaaacg aatctccaaa
     1381 gaagagggcg tcaaacttct acggccggga gaaatcgttt tctgcgttta cgatcgtgaa
     1441 agtaccaacg aacccaaccg cttggtcgct gcttccgttg gggtcgctat tccagcagac
     1501 caggagcagc atggttatct ttcggagcac cattcattcg gagaaaccga agagaaagcc
     1561 ggcgattacg ccgaagattt agcagccagc atgcttgcca caacactggg catcgaattt
     1621 gatcccgaca ccgcgtggga tgagagggaa aacctcttca agatgtccgg aaaaattgtt
     1681 cgaacttcca atatcacgca atccgcaatt ggtaataagg atggtctttg gaccacggtg
     1741 ttcgctgcct gcgctttcgt taacgacgac aattaactga atatcgttta ttcagcgata
     1801 gccgagtctc cgcacccggc cgtcctgtga tgggctcgca cacttcactg cgtgcttctc
     1861 ggcctggcgg catacgccac ggctatgctc acgccataaa gcaaacacaa aggtaacatc
     1921 aacaagatct gcgaaatcag atccggcgga gtgagcacgg ccgagatgat ggccatgccg
     1981 atgactgcgt agccaaaata gtggattaat aggcgatggt ccaccaaacc cacacgggtg
     2041 aggaagaaac tgagcaccgg caactgaaac atcagtccaa atgccagtac cagccttgca
     2101 accatcgaaa ggtagtcacc aatttgaatt gctggctgta tctgcagcac ctgatatcgc
     2161 tttaaaagga gagcgagtcc tttgtgtaaa acgataccat aacaaaaagc cacgcctgcg
     2221 agaaaaaaaa acgagccaaa aagcacaaag ctgcgggtat aacgcttctc atgctcgtat
     2281 aggcccggtc caacaaattg ccagccttgc cacaacagca ccggcagcgc aacgatcagc
     2341 gcggcgaaaa acgcaatctt catttttgtg aagaaggctt cggtcacggc cgtgccgatc
     2401 atagtcagat ccggtattcg aaggcgccgg agtggttccg atagagcaaa gaaaatatgg
     2461 tcggcgaaat agaagcagaa gacgaaagca atagcgacag ccaggacgga gcgaatcagt
     2521 ctggagcgga gctcttctaa atgagaacta aaaggcatct gaacattgtc caaatgcctt
     2581 ttagcggaag cgtcactcac gtggttcgtt aggaggcgtg gcggatgcca ccgaaacatc
     2641 agcttgcttg gttagtagcg gatcgtcttt ttgagccgcc tgagcggact gttcaggtgg
     2701 ttcattttcc aattgacgaa actcgtcttt gagctcatca gttgcccgtc taaactcagc
     2761 catgcccttg ccgagggcgc gagcgagttc aggcagtttc ttgggaccta aaacgatcag
     2821 cgccaaagct aagatcagca atagctccgg catcccgata ccgaacatgc tctatcctcc
     2881 ttgttcaggc tatgcgattt tctatgagtt ggcaaccaag ctaagtgtct cgacaacgac
     2941 ttccccagct tccagtaggt tccttctgct gggcaacggt gtcaacgatt agcccgcgcg
     3001 aaacgttgtg accggtagcg tcgaatgcta gcttaagggg gatgagacgt tctgcgattc
     3061 taacagatcg agagtttctc aagcacatgc cgggtcgggg tcatcccgaa cgcccggagc
     3121 ggctgcgggt tttgctggac ctggccgaga ccctagatcc agctcgtttt cagctgttgc
     3181 ctcccaga
//
LOCUS       DASUTG010000213         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1664676, whole genome shotgun sequence.
ACCESSION   DASUTG010000213 DASUTG010000000
VERSION     DASUTG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664676"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1550
                     /locus_tag="VF089_04775"
     CDS             462..1550
                     /locus_tag="VF089_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173306.1"
                     /translation="MAGVIDADTHIAESEAMWKFIDREMYPRRPVLAKIPDDTWYKDR
                     NAFWLIDGEIFPKPAGKGSFSLITPSAAKREAGRGDIHLAVRELTDPMARLKDMDRIG
                     VEVQVIYPTLFLVYLTDDPRLEVALCRAYNRFVADACAKAPERLKWVAILPLRAVDSA
                     LEEMTWAKEHGAVGIFFRGMEGNLTLDNPYFFPVYAQAEQLDLPICIHTGSGSRHLMQ
                     LFDVERNHTFAHNRVLPVFAFRDLVHNRIPEKFPSLRFGFIEAAASWVPYIWHVLRRQ
                     QSNWRFSNPQELFREYRFWVAREADEELPHLVRFLGEDKLIIGSDYGHNDPSKEPQFV
                     ANLRGREDLPSVLVEKILCQNPRAFYGL"
     gene            1727..3277
                     /locus_tag="VF089_04780"
     CDS             1727..3277
                     /locus_tag="VF089_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173307.1"
                     /translation="MTNPSQEYTILNVDDHDAGRYAKSRILQLAGYRVVEACTGADAL
                     RLVREAKPQLILLDVKLPDVNGIQLCRTIKSDPLSAHIMVLQISAVHTTRADRIYGLE
                     CGADTYLIEPVEGDELLATITALLRLYDRERENRRLVAQLRDADRQKDDFLAALAHEL
                     RNPLGVVSNAFDILPETQTQEPVSQELREIIGRQISLMTHLVDDLLDVSRISRGQIGL
                     VREPCDFAAIVRQTVDDHRAILESNGLELKLEVPSHPVWVVGDATRLAQSISNLLQNT
                     GKFTSPGDTVTVKLVDLPDEHRAVLSVRDTGIGMESDFLARIFEPFRQFDPGVGKRQG
                     GLGIGLSLVKGLIELHGGEVHARSEGLGHGSEITIRLPTHKFTDTSDLSSKPSKTDAD
                     RPASYRILVIDDNSLAGRTLQIFLADKGHKVELAVTGPQGLEKARQFLPQVVLCDIGL
                     PGLDGYSVARQLRQEQGSTKMFLIAVSGYGQEEDQQRAKTAGFDAYLVKPISLKDLEK
                     LLAEFNGR"
     gene            complement(3332..>4560)
                     /locus_tag="VF089_04785"
     CDS             complement(3332..>4560)
                     /locus_tag="VF089_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173308.1"
                     /translation="AGLVVERGTAVRLKIQNTLSPLLDPGFLEGALRRHFQPLLDLRF
                     DEFLAEHYPKGVAIYINGEQLDRHLWAAPLHAPLSIRLSRKRKPSALGYLIREENSLP
                     EERRGLAISTYGKVIRRGWDWLGMTPMSPERIGGFIEVPELAACLTLNKGDFIRAGAR
                     GAIYLAFRKAIQEAVSRQLATWGDARASTDEAPPREVRPLQRDLAYVLEDLAIDFPLL
                     ASLVEQRSGGQKKLPLGQASNATESRAFVAASIAEQSEAFADQLEPTSSSQSQNGDKS
                     RNDTPATESVTAPGHGLLLPAQAAVRRPGHYGLDIQFEDLPEALELAHLMESTVWVNR
                     AHPAYRRALASRSVGYHIALAVAMALAPLAVESANEHAFVTAFLFRWGEAIENTTTRK
                     QKRLGGQARRPASTKQ"
BASE COUNT         1008 a         1199 c         1334 g         1019 t
ORIGIN      
        1 aaagggctgg aatttatcat ttcgcaggtg gccaccacta accccaaagc caggggtcag
       61 acgcctgaga gtctgaggct cctcgatggc agcgtcttgg aagaaatcaa gaagagcgga
      121 ttcatcgaga aatttagaaa gtaggcctta aaggcgatta ccaccgggaa atgtgtcgtg
      181 accttgcgct cgcctacaga gaagcaaagt ggggtacgtc catcaagccg ctgattctcg
      241 gacactctgt cattcccgca tgcttctagc gggaatccag gcggaattgg gactggaccc
      301 ccgattaaag catttggggg tgacgatgcc ggtaaggggc atctctcacc tcagccgcac
      361 tcttttaagg agctcacgag gagcacgagg ggttgcaaaa ctcgccatcg acgatttcaa
      421 cgttttaatc ggcttatctt ttcagggcag gaggcagaga gatggctggt gtgatcgatg
      481 cagatacgca tatcgccgag tccgaggcaa tgtggaaatt tatcgatcga gagatgtatc
      541 cgcgcagacc tgtcctggct aagattcccg acgatacctg gtacaaggat cgaaatgcat
      601 tttggctgat cgatggcgag atcttcccca agccggcggg caagggaagc tttagtttga
      661 tcacgccctc ggcggcgaag agagaagccg gaaggggaga cattcacctc gcggtgcgag
      721 aattgaccga tcccatggcc cgtctgaagg acatggacag gatcggtgtg gaagtgcagg
      781 tgatttatcc gacacttttc ctggtgtatc tcactgacga tccgcgcctg gaggtagctt
      841 tgtgccgtgc ctacaatcgt tttgtcgccg atgcctgcgc taaggcgccg gaacggctca
      901 agtgggtcgc gatcctgcct ttgcgcgccg tcgattcggc gttggaagag atgacctggg
      961 ctaaggagca tggtgcggtc ggtatatttt ttcgcggcat ggaagggaac ctcacgctcg
     1021 ataacccata tttctttccc gtttacgccc aagcggagca gctcgatctg ccgatctgca
     1081 ttcacaccgg ttcaggcagc cggcatctca tgcagctctt cgatgtcgag cgcaaccaca
     1141 cctttgcgca taatcgggtg ctgccggttt ttgccttccg cgatttggtg cacaatcgga
     1201 ttccggagaa gttcccgagc ttgcggttcg gttttatcga agctgccgcc agttgggtgc
     1261 cgtacatctg gcatgtgctg cggcgccagc aaagcaactg gagatttagt aatccgcagg
     1321 aactctttcg cgagtatcgc ttctgggtgg cccgcgaggc cgatgaagag ttgccgcacc
     1381 tggtgcgatt tctcggcgag gacaagctaa tcatcggctc cgattacggc cataacgatc
     1441 cttccaagga gccgcagttt gtcgccaacc tgcgcgggcg agaggatctg cccagcgtct
     1501 tggtcgagaa gattttgtgc cagaatccac gagccttcta tggcttatga tgagatcgaa
     1561 atcgtctcac ggcgcgcgga caatatgtcc cgcatagaaa ctgtgttggc cgagcatcac
     1621 gagcctaaat cggatagttt tcgcggatat tcttgatgat cagggggaaa ttctgtattt
     1681 gaagcgcatt gaccatcaac gacgcaagtg attgcgccag atctaaatga caaatcctag
     1741 tcaagagtac acgattctga atgtcgatga tcacgatgcc gggcgctatg ccaagagccg
     1801 tattcttcaa ttggcagggt accgggtggt tgaggcctgc actggagcgg atgccttgcg
     1861 cctggttcga gaggcgaaac cccagctgat tctactcgat gttaagttgc cggacgtaaa
     1921 cggcatccaa ctgtgccgca cgattaaaag cgatccgctg agcgcccata ttatggtcct
     1981 gcaaatctcg gcggtgcaca cgacccgggc tgatcggatt tacggcttgg aatgtggcgc
     2041 agacacatat ctgattgagc ccgtcgaggg cgacgagctc ttggcgacga tcactgcgct
     2101 gctacgcctc tatgatcgtg aacgggagaa tcgtcggctg gtggcacagt tgcgcgacgc
     2161 cgatcgtcag aaagatgatt ttttagctgc cctggcgcac gagttgcgga atccgcttgg
     2221 ggttgtcagc aatgcgtttg atattctccc cgagacgcaa acccaggagc cggtgtcaca
     2281 ggagcttcgc gaaatcatcg gccgccagat cagtctaatg acccatctgg tcgatgactt
     2341 gctcgatgtc tcgcgtatct cgcgcggtca gatcggactt gtccgtgaac cctgcgactt
     2401 cgctgctatc gtgcgtcaaa cggtggacga ccaccgagct attttagagt caaatggtct
     2461 ggagctaaaa ctcgaggtgc cgagtcaccc ggtgtgggtc gttggtgacg ctacccgtct
     2521 ggcgcaatcg atcagcaatc tcctgcaaaa caccggtaag tttaccagtc ccggggatac
     2581 ggtgacggta aagctagtcg accttccaga cgagcatcgc gcggtgctga gcgttcggga
     2641 caccggcatt ggtatggaat cggatttcct ggcgcggatt ttcgagccat tcaggcagtt
     2701 tgatccaggt gtcggaaaga ggcaaggcgg gctcggcatc ggtttgtcgc tggttaaagg
     2761 gttaatcgag ctgcatggcg gagaagtgca tgccagaagt gaaggtctag gccacggttc
     2821 agaaataact atccgcctgc cgacccataa atttactgat acctctgacc tgtcttccaa
     2881 accgagcaaa actgatgctg atcggccggc ttcttaccgt attctggtga tcgatgacaa
     2941 ctcgttggca ggtcgtacgc tgcagatttt cctcgcggac aaaggacata aggtcgagct
     3001 tgccgtcacg ggcccgcaag gcctagaaaa ggcacggcag tttttgccgc aggtagtgct
     3061 ctgcgacatc ggcctgcccg gactcgatgg ctactcggta gcgcgtcaac tgcggcagga
     3121 acagggctcg actaaaatgt tcttgattgc ggtctcgggt tacgggcaag aggaagatca
     3181 gcaacgtgcc aaaactgccg gcttcgacgc ctacctggtc aagccgatta gtctaaaaga
     3241 tctggaaaaa ctcctcgccg agttcaatgg acgttagaaa ccggagccat gggtaagtgt
     3301 ttacggcggc tttcctaccg cgatttgggc cttattgttt tgtagaggca ggcctgcgtg
     3361 cctgccctcc aagcctcttc tgtttgcgtg tggttgtgtt ttctatggct tcaccccagc
     3421 gaaagagaaa cgcggttacg aaggcgtgtt cgttggcgga ctctacagcg agcggggcta
     3481 gggccatcgc cactgccagc gcgatgtgat agccgaccga acgcgaggcc aatgctcgac
     3541 gataagccgg gtgagcgcgg ttaacccaaa ccgtcgattc catcaaatgg gcaagctcca
     3601 acgcctccgg taggtcttca aactgaatat caagcccgta atgtccgggc cgccggacag
     3661 cggcttgcgc cggcagcagc aagccatgac ccggggccgt tacggattca gtcgctggag
     3721 tgtcgttacg acttttgtcg ccgttttggc tttgcgacga tgacgtgggc tcaagttgat
     3781 cggcaaaagc ttccgactgt tcggctatcg atgccgccac gaaagcgcgc gactcagtgg
     3841 cattggatgc ttgtccaaga ggcaattttt tctgtccacc cgagcgttgt tctaccagcg
     3901 acgcaagcag aggaaagtcg atggcaaggt cttcgagcac gtaggccaag tcgcgttgta
     3961 gagggcgaac ttctcgtggt ggcgcctcat ccgtggatgc gcgggcatct ccccaggttg
     4021 cgagttgcct ggaaacggct tcctgaatgg cctttcgaaa ggccaaatag atggctccgc
     4081 gcgcgccggc gcgaatgaag tcacctttat tcaaggtcag gcaagctgcc agttccggta
     4141 cttcgataaa tccgccgatc cgctccggag acatcggcgt catgcccagc caatcccaac
     4201 cgcgccgaat cactttacca taagtgctga tcgctaaacc gcgcctttct tccggcagtg
     4261 aattctcctc gcgaatcaaa tagccgagcg ccgatggttt gcgcttgcgt gacaatcgaa
     4321 ttgacaatgg cgcatgcaga ggagcagccc agagatgcct gtctagctgc tcaccattga
     4381 tatagattgc aacgcccttg ggatagtgct cggcaagaaa ctcgtcgaat cttagatcga
     4441 gcaaaggttg aaaatgacgg cgcagagcac cttccaggaa accggggtcg agaagagggg
     4501 ataaggtatt ctgaattttc aggcgaactg cggtgccgcg ttcaacgacc agaccggcgg
//
LOCUS       DASUTG010000214         4784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1219803, whole genome shotgun sequence.
ACCESSION   DASUTG010000214 DASUTG010000000
VERSION     DASUTG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219803"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VF089_04790"
     CDS             <1..389
                     /locus_tag="VF089_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173309.1"
                     /translation="TLGILADEGFLWHGDAVNDDNPYPVDINGKTLIVVPYKNAISGL
                     NDTGMYRRGITARDVFNSFKDEFDVLYEESLEDPKMLTLAMHCQMAFPATGKVYDEAI
                     QYAKSHDGVWFAKRIEIVNWVKENCL"
     gene            536..1933
                     /gene="leuC"
                     /locus_tag="VF089_04795"
     CDS             536..1933
                     /gene="leuC"
                     /locus_tag="VF089_04795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX6173310.1"
                     /translation="MAGKTLYEKIWESHTVGVLPTGQTQLFIGLHLIHEITTAPAFDM
                     LREQGMDVAFPERTFATVDHIVPTDVRSRPFLDREAEELTQALEKNVKQFGVEFFGLD
                     SADQGIVHVIGPQLGLTQPGMTLACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQTLA
                     MESLKVRRINIDGALGAGVYAKDVILNIIRRLGVAGGKGFAYEYGGSVIDSMNMEERM
                     TVCNMSIEGGALVGYVNPDATTFEYLNGRRHVPVGAAFDRAVAYWKSIASDADAKYDD
                     VINFAGKDIEPTVTWGINPGQAVGVTEKLPKPDQYSDPAGATKAYEHMGWQPGAPILG
                     TPIDVAFIGSCTNGRLADLRAAAKIAKGRKVQSGIRALVVPGSAEVKKLAEQEGLHEI
                     FKEAGFEWREAGCSMCLAMNPDKLHGKEISASSSNRNFIGRQGSPQGRTLLMSPAMVA
                     AAALNGKVVDVRDYQ"
     gene            1943..2557
                     /gene="leuD"
                     /locus_tag="VF089_04800"
     CDS             1943..2557
                     /gene="leuD"
                     /locus_tag="VF089_04800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012943908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX6173311.1"
                     /translation="MNEIVRITNVEGTAVTIRGNDIDTDRIIPARYLKEVSFARMGEY
                     PFFDERFDASGNLKPHPLNDAKYKGASLLFVNKNFGCGSSREHAPQALYRWGIHAIIG
                     ESFAAIFAGNCTMMGVPTVTVSPGEIENLMQAVEKDPTTMFRLDLQAKTISFGEHKIA
                     VDLPETYRSALISGSWDSTAMLRANLSEVKKTAAKLPYMNNFAG"
     gene            2707..3012
                     /locus_tag="VF089_04805"
     CDS             2707..3012
                     /locus_tag="VF089_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX6173312.1"
                     /translation="MADKWDERKKAQEDEYFVKKERELLAKLKAKQEAESKATGKNAA
                     HMTCPKCGEPLKARSFQKIEIDQCTGCNGIWLDAGEMEQVAGKENDSWLGRFWQKNG"
     gene            3081..3671
                     /locus_tag="VF089_04810"
     CDS             3081..3671
                     /locus_tag="VF089_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6173313.1"
                     /translation="MKQTPRLNSIALVLLLIAPAVFNFGVARAQEGKIVPYVPTPQEV
                     VERMLELAQIKKGDVLYDLGSGDGRIVVTAAKKYGVRAIGFEIDPERIKESTENIKKA
                     GVGHLVEIRQQDIRTVDLSPASVLTMYLLPEVNLMIRPNIWKQMKPGSRVVSHDFDMG
                     DWKPLKTENMKDSSNWDHTLYLWHVQGAEKQAGDSR"
     gene            3980..>4784
                     /locus_tag="VF089_04815"
     CDS             3980..>4784
                     /locus_tag="VF089_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173314.1"
                     /translation="MIIAAAFLLHSFVAAAEIKIGVAAALTGNAAQYGVPIRKGFELA
                     IREINGSGGVAETRLELVIEDEQGKKEEAINSFRKLIFQDKVLMLFGPTLSNSAQASD
                     PVAQAAKTVVFGTSNTADGITSIGDYVFRNSVTERDILPVTLKIAAQKTGLKKVAVLY
                     GNDDIFTKSGYDNFKKALHDLKIPVTATETFAKGDVGFKPQLTNIKASSPDAIVLSAL
                     VAEGAPIMVQARQLGIDLPFIGGNGMNSPRVFELAKNSSDNLWVGSPWSA"
BASE COUNT         1206 a         1147 c         1354 g         1077 t
ORIGIN      
        1 gaacgctggg cattcttgcc gatgaaggtt ttctctggca tggggacgcc gttaatgacg
       61 acaatcccta tccggtcgat atcaacggca aaacgttgat cgtggtgccc tataagaacg
      121 cgatcagcgg actcaacgac accggcatgt atcggcgagg catcaccgcc cgtgatgttt
      181 ttaattcctt caaggatgaa ttcgacgttc tttatgagga gtccctcgag gatcccaaga
      241 tgctgacctt ggcgatgcat tgtcagatgg cgtttccggc gaccggcaag gtctacgacg
      301 aggcgattca atacgccaag tctcacgatg gcgtttggtt cgccaaacgt attgagattg
      361 tgaactgggt aaaggaaaat tgtctctaaa tcaattaatg ctctgactgc atccgccggc
      421 cgccccgatt atcagtgaat cgtaaaatga ataaagtact gaccgaaagc accggaactg
      481 atccacttga agtcgcggat agcggcagat agtatcctgc gaaggagatt tgccaatggc
      541 cggaaagacg ctgtacgaaa agatttggga atcccatacg gttggggttc ttcccacggg
      601 ccagacgcag cttttcattg ggctccatct gattcacgaa attaccacgg cacctgcttt
      661 cgatatgttg cgagagcagg gaatggatgt ggcgtttcct gagcgaactt tcgctactgt
      721 ggatcatatt gttccgaccg atgttcgtag ccggcccttt cttgatcgtg aagccgaaga
      781 actgacccaa gcgctcgaaa agaatgtcaa acagttcggc gtggaattct tcggtttgga
      841 tagcgccgat caaggaatcg tccacgtcat cgggccgcag ctcggtctga cccaaccagg
      901 aatgaccttg gcgtgtggcg acagccacac gagcacccac ggagctttcg gcaccttggc
      961 atttggcatt ggtaccagtc aggtgcgcga cgttctggcg acacagacct tggcgatgga
     1021 aagcctcaag gtccggcgca tcaatatcga tggcgccctc ggcgccggtg tttatgccaa
     1081 agacgtaatc ctgaacatca tccggcggct cggcgttgcc ggtggaaaag gtttcgcgta
     1141 cgagtatggc ggttctgtga tcgattcgat gaacatggaa gagcgtatga ccgtctgcaa
     1201 tatgagcatt gaaggcggtg cattggtagg ttatgtcaac ccggacgcta ccacgtttga
     1261 gtatctcaat ggcaggcgcc atgtgccggt tggcgccgca ttcgatcgtg cagtggccta
     1321 ttggaagtcg attgcgtccg atgcggatgc caaatacgac gatgtcatca acttcgcagg
     1381 aaaagatatc gaaccgacag tcacttgggg tatcaatccg ggacaagcgg tcggcgtcac
     1441 cgagaagctt ccaaaaccgg atcaatactc cgacccagct ggcgcaacaa aagcctacga
     1501 gcatatggga tggcaaccgg gcgcgccaat tctaggcacg ccgattgatg tggcgtttat
     1561 cggctcgtgc actaatggcc gactcgcgga tctgcgggcg gcggctaaga tcgccaaggg
     1621 aaggaaggtt caatccggaa ttcgcgcgct ggtggttccc ggttccgccg aagtcaagaa
     1681 actagccgag caagaggggc tgcacgaaat atttaaggaa gcgggatttg aatggcgcga
     1741 agcgggatgt tcaatgtgct tggcgatgaa tccagataag ctccatggca aagaaatctc
     1801 ggcgtcctca tcaaatcgta acttcattgg ccgtcaaggt agtccgcagg gaagaactct
     1861 gctgatgagc ccggccatgg tcgccgctgc cgcgctgaac ggtaaggtcg tcgacgtgcg
     1921 ggattatcaa taagaggcga ctatgaacga aattgtgaga atcaccaacg tagaaggcac
     1981 ggcagtgacg attcgcggca acgatatcga taccgatcga atcattccgg cgcgttatct
     2041 taaggaagtg agctttgccc ggatgggtga gtatccattc ttcgacgaac gttttgatgc
     2101 gtcgggcaat ttgaagcctc acccgctcaa tgatgccaaa tacaaagggg cctctttgct
     2161 tttcgtaaat aaaaactttg gttgcggctc gtcgcgcgag catgcgccac aggcgctgta
     2221 tcgatgggga atccatgcca tcatcgggga gtcgttcgcg gctatctttg ccgggaactg
     2281 cacgatgatg ggggtgccga cggtaacggt tagtccgggg gagattgaaa acctgatgca
     2341 agccgtcgag aaggatccga caactatgtt tcgtttggat ctccaagcca agactatctc
     2401 gtttggcgag cacaagattg cggttgattt accggagacg tatcgaagcg cattgatcag
     2461 cggttcctgg gattcaacag caatgcttag agccaacctg agtgaggtaa agaaaactgc
     2521 agcgaagctt ccttatatga acaattttgc cgggtagacc acgatcaatc atccaagcgc
     2581 cgccattgtg tttggcattg cgttgagcga cgccgcattg tttctgccct cgtgcatccg
     2641 attcgcaggt caatcggtgt ttaccttgaa ccttctgaaa agtatgagaa tacgtaggag
     2701 gacgacatgg ctgataaatg ggacgaacgt aagaaggcgc aagaagatga gtattttgta
     2761 aaaaaagaga gggagcttct cgctaagctc aaggcaaaac aggaggctga gagtaaagcc
     2821 accggcaaaa acgcggcgca tatgacatgc cccaaatgcg gcgaaccgtt aaaggccagg
     2881 agcttccaaa agatcgagat cgatcaatgt accggatgta atggcatttg gctggacgcc
     2941 ggtgagatgg agcaagtcgc gggcaaggaa aatgacagtt ggctcggcag attttggcag
     3001 aagaacggtt gatcgtttgg gtatctgttg acagttgata actattcgag ccatcgatta
     3061 aacatcctga agagcccgca atgaagcaaa ctccgcgcct aaactctata gctctcgtac
     3121 ttctgttgat cgcgccagcg gttttcaact ttggtgtagc gcgagcgcag gaaggaaaaa
     3181 ttgttcccta cgttccgacg ccgcaggagg tggtcgaacg catgctcgag ttggcccaga
     3241 ttaagaaagg tgatgtgtta tatgatcttg gttccgggga tgggagaatc gtcgtcaccg
     3301 ccgccaaaaa gtacggagtt agggcgatcg ggttcgagat cgatccggaa cgaatcaagg
     3361 agtccacaga gaatatcaag aaggccgggg ttgggcattt ggtggaaatt cgccagcaag
     3421 acatcaggac tgtcgattta tcaccagcct cggtgctgac gatgtacctc ttgcccgagg
     3481 tcaatctaat gatccggcca aacatctgga agcaaatgaa acctggttcc cgggttgtgt
     3541 ctcacgactt cgacatgggc gactggaaac cgctcaaaac ggaaaacatg aaagattctt
     3601 caaactggga tcacacgcta tatctttggc atgttcaagg cgcggaaaag caagccggcg
     3661 atagtcgata gctgcggatc gcgcatggcg atcggtttac ccctggcctt ctattgataa
     3721 agcttacatg gaatcgtggc tctagcgcct ggtcttgatg gtccgcaagc tgaaaatcat
     3781 cgacccaatt ggcttgaact cagatcagcc gctatgatct gagcacccta aaggttaaga
     3841 tctcacaggc aatcgtgcac gcgccggcgt ggcaggctta atttttagtc agggcgattg
     3901 tagaaggcga gacttcgtct gacgtggtca aagtattttg caacaggtgt ccttcgtgaa
     3961 gctgaaacac actatacgga tgataattgc agcggctttt ctgcttcact ctttcgtcgc
     4021 ggcggcggag atcaagatag gggtcgccgc cgcattgacc ggcaacgcag cgcaatacgg
     4081 tgtgccgatc cggaaaggct ttgagttggc cattcgcgaa atcaacggca gcggcggagt
     4141 cgctgagacc cgattggaat tggtcattga agatgagcag ggcaaaaagg aagaggctat
     4201 caatagcttt agaaagctta tttttcagga caaagtgctg atgttgttcg ggcccacgct
     4261 gtctaattca gcgcaagctt cagacccggt agcgcaggcc gcaaagaccg tcgtcttcgg
     4321 aacttcgaat accgctgacg gaatcacttc gattggcgac tatgtttttc gtaattcggt
     4381 taccgagcgc gacatcttgc cagttacgct taaaatcgcc gcacaaaaaa ccggtttgaa
     4441 aaaagtcgca gtgctctacg gtaatgacga tatcttcaca aaaagcggct atgacaactt
     4501 caagaaagct cttcacgact tgaagatccc agttacggcg actgagactt tcgcaaaagg
     4561 cgacgtcggc tttaagccgc aactgaccaa tatcaaagcc agcagccctg acgctatcgt
     4621 gctttcggcg ttggtggctg aaggggcgcc gatcatggta caggcgcggc aattgggcat
     4681 tgacttgccg ttcatcggcg gtaatgggat gaactctcca agagtattcg agcttgccaa
     4741 gaacagctct gataatttat gggttggcag cccctggtcc gcgg
//
LOCUS       DASUTG010000215         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1219837, whole genome shotgun sequence.
ACCESSION   DASUTG010000215 DASUTG010000000
VERSION     DASUTG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219837"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF089_04820"
     CDS             complement(<1..221)
                     /locus_tag="VF089_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173315.1"
                     /translation="MSSIGQKMRRSTESVPPGRGQPTKTRPGEGKGAEQWKMPPGRTW
                     LWFVGILLANYLLVRFLVPGPEAPIAVPYT"
     gene            complement(507..1958)
                     /gene="cls"
                     /locus_tag="VF089_04825"
     CDS             complement(507..1958)
                     /gene="cls"
                     /locus_tag="VF089_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX6173316.1"
                     /translation="MDNIFTVLGTALALYGLVVSVFVITENRRPQSTLAWMLILFFAP
                     GMGLLIYLFFGRDGKAFSKRSKLLMQDLEANARPLLSPILSRQDGELARLSNETLAGK
                     KLAMLVRRNSRSVLTTRNTVEIQQDAARFYPSLIEDLKAARHSIHLQYFIWGVDPFTE
                     SVREILIEKASAGIEVRLLYDPQGSQAHVGRRYVKEMLANGLRMAPTSPLYHLHTISY
                     RNHRKITVIDGVIGYTGGMNMGQEHLDGGKGFDSWRDTQVRLVGESVTILQAVFMVDW
                     YNAVGENLFQAKYFPTDAAEAEENNIPVQILTSGPDSQWAAIRQLYTLMVVSARQHVY
                     LQSPFFLPDATLAEALTEAALSGIDVKVMVSARPSGNRLPDWAGHTFIEEIVKAGVRV
                     FLYDKGYLHAKTISIDSEICSIGSANIDIRSFSINYELNAVFYSRQIAKQLEADFERD
                     LAHCKEFDPEEYRKRGTVERFRDSVARLLSPLL"
     gene            complement(2020..>3520)
                     /gene="nhaA"
                     /locus_tag="VF089_04830"
     CDS             complement(2020..>3520)
                     /gene="nhaA"
                     /locus_tag="VF089_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX6173317.1"
                     /translation="NLPPTDGIHQEAWRRAQTKVQEDIDSARRSGARVSPTFFINNRR
                     YEGPWDGSALTEAFLGSLGHRVQTAALDFARWAPSTGLLLLTMIIAALLLTNSPAGRA
                     FEALWEMPVGWNAGGRSFQLPLRDWFNDGLLTIFFLVVGLEIKRELTVGRLSTARVAA
                     LPLAAAAGGMLLPASLYLLLVPAGPLAAGWAIPTTTDTAFAVALIAFLGARVPVELRI
                     FLTAAVVVDDLVAIAILALFYSAGIDLLYTTASIAATAVMVLFNRGGIYAPLPYAALG
                     IVLWGCLHGAGLHPTLAGVIVAALTPTRPPGNLRALMAQAETVIGHELRRAEEGVLRH
                     GPSEPALRALDAIHDRIESPASKLLRSVEPWSSYFVLPLFALANSGLDLSAVVLQGHE
                     RLVSAIVLGLVLGKPIGMALGAALAVGLGIAVKPEEYSWRQMIGAGALAGIGFTMSLY
                     IAHKAFLDESDFAAAKVGIFLASLIAGGLGTALLWRAAKSQSGKDARAT"
BASE COUNT          746 a         1062 c         1031 g          681 t
ORIGIN      
        1 gtataaggaa ctgcgatcgg cgcttccgga cctggtacga gaaacctcac cagcaaatag
       61 ttcgctaaca ggatgcccac gaaccagagc caggttctcc caggcggcat cttccactgc
      121 tcggcacctt tcccttcgcc aggtctagtc ttcgttggct ggccgcgccc gggcggcacg
      181 gactcagtac tgcgccgcat cttctgtccg atcgaactca tagtcgcctc ctcggatacg
      241 ctctggctaa tggtaggcta acatcatgcg gtatcgattg acgactatca tccacaccca
      301 atgacaacaa ggtgaattta tcgtttttgc ttgaactctg tccgttgatt ttagcagcag
      361 agcacgagaa tttcggtagt gctcaccgcc aggctgccaa ggcatgagcg gttagctcaa
      421 catgtgcaga agacaacttg gaggtaacgc atccctacgg ttcttatcgg ttctcggcga
      481 ctggatcaga agactgagtt tcacactcac agcaagggag agaggagcct tgcaaccgaa
      541 tccctgaagc gctccacggt gccgcgcttg cgatattcct ctgggtcgaa ctccttacaa
      601 tgagcgaggt cgcgctcgaa gtccgcttcc agttgtttgg caatctgtcg gctgtagaat
      661 actgcattga gctcgtagtt gatgctgaag ctgcggatat cgatgttggc cgagccgatc
      721 gagcagattt ccgagtcaat gctaattgtc ttggcgtgca aatagccctt gtcgtaaaga
      781 aacacgcgca cgcccgcctt gacgatctct tcgataaagg tgtgccctgc ccaatccggc
      841 agcctgttac ccgacggccg cgcgctcacc attaccttga cgtctatccc cgataaggcc
      901 gcttctgtca gagcttcggc gagcgtcgca tcaggaagga agaagggtga ctgaagatag
      961 acgtgttgcc gcgcggaaac taccatcaga gtataaagct gtctgatcgc cgcccactgc
     1021 gaatccgggc ccgaggtcag gatctgcacc gggatattgt tttcttccgc ctctgcggcg
     1081 tcagtgggaa agtatttggc ttggaagaga ttctccccga ccgcgttgta ccagtccacc
     1141 atgaacaccg cctgtagaat ggttacgctt tctcccacca gccgcacttg agtgtctcgc
     1201 cagctgtcaa atccttttcc cccgtcgagg tgctcctggc ccatattcat gccgccggta
     1261 tagccgatca ccccgtcgat aacagtgatc ttgcggtgat tgcgataact gatcgtatgc
     1321 agatgataga gcggcgatgt cggcgccatt cggagcccgt tcgccagcat ttctttgaca
     1381 tagcgccgcc cgacatgtgc ctgagagcct tgcgggtcat aaagcagccg tacttcgatg
     1441 ccggcgctcg ccttctcgat caagatctcc ctaacgcttt cggtgaacgg gtcgacgccc
     1501 cagatgaaat attgcaaatg aatcgagtgc cgcgcagcct tgaggtcttc gatgaggctg
     1561 gggtaaaacc tggctgcatc ctgctgaatc tcgacagtgt tacgggtggt cagcactgaa
     1621 cgagagttcc gtcgcaccag catggccagc tttttaccgg caagagtctc gttcgacagg
     1681 cgcgccagct cgccgtcttg acgggacagg atcggtgata acagcggccg tgcgttggcc
     1741 tcgaggtcct gcatcagcaa cttgctgcgc ttgctgaaag ctttgccgtc tctgccgaaa
     1801 aaaagataaa tcaggaggcc catccccggc gcgaaaaaga gtatgagcat ccatgccaga
     1861 gtcgattgcg gcctgcggtt ctcggtgatc acgaacacgc tcacaaccag cccatagaga
     1921 gccagcgcag tccccaagac ggtgaagata ttgtccatga gcatggctgc tgccaggcgc
     1981 agtgtgtcgg cgatgcgcgc cttccgcggc cggtgctcgt caggtcgcgc gtgcgtcctt
     2041 gcccgactgc gattttgcag cgcgccaaag cagcgcggtt cctagcccgc cggcgatcaa
     2101 tgaggcgaga aaaatcccga cctttgctgc ggcgaaatcc gactcatcga gaaatgcctt
     2161 gtgggcaatg tagagcgaca tggtgaaacc gatcccggca agcgccccgg cgccgatcat
     2221 ctgacgccat gaatactcct ccggctttac ggcgatgccc aggcctacgg cgagcgcggc
     2281 gcccagcgcc attccgatgg gcttgcccag caccagcccg aggacgatgg cggagacgag
     2341 cctctcgtgg ccctgcagga ctacggcgga caagtcgagg ccggagtttg cgagagcaaa
     2401 cagcggcagc acaaaataac tcgaccacgg ctctaccgat cgcagcagct tcgaagcggg
     2461 cgattcgatt cggtcgtgaa tcgcatccag cgcccgcaat gccggttcgg aagggccatg
     2521 ccgcaaaacc ccttcttcag cgcggcgcag ctcgtggccg atcacagtct cggcctgggc
     2581 catcaaggcc cggaggtttc ccggcggtcg ggtcggggtc agggcggcga cgatgacgcc
     2641 ggccagggtg ggatgcaagc cggcaccatg caggcagccc cacagaacga ttccgagcgc
     2701 agcgtagggc agcggtgcgt agatgccgcc gcgattgaac agtaccatta ctgccgtcgc
     2761 agcaatggag gcggttgtat agagcagatc aattccagcc gagtaaaaca acgccagtat
     2821 cgcgatggcg acgagatcat caacgacaac cgccgcggtg agaaaaatcc gcaactccac
     2881 cggcacgcgc gcacccaaga acgcaattag cgcgaccgcg aaggctgtat cggtggtcgt
     2941 tggaatggcc catcccgcag cgagcggacc ggccggcacc agcagcagat atagggacgc
     3001 gggaagcagc atgccgccag cggcggcggc cagcggcaag gcggctacgc gagctgtgga
     3061 caaacgtccg accgtcagct cgcgctttat ttccagcccg acgacgagaa aaaaaatcgt
     3121 cagcaagccg tcattgaacc agtctcttag cggcagttgg aatgacctgc cgccggcgtt
     3181 ccaccccacc ggcatttccc acagcgcttc gaacgcgcgg cccgcgggcg aattggtcaa
     3241 taggagcgct gcgatgatca tcgtcagcag cagcaaaccg gtggacggag cccatcgcgc
     3301 gaaatccaga gccgcagtct gcacgcgatg gccgagggag ccaaggaagg cttccgtgag
     3361 cgcgctcccg tcccagggtc cttcatagcg gcggttgttg ataaaaaacg ttggcgacac
     3421 tcgggcgccg ctgcgccgcg cgctgtcgat gtcctcttgc accttggtct gggcgcggcg
     3481 ccatgcctcc tggtggatgc catcagtcgg cggcaggttg
//
LOCUS       DASUTG010000216         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1220182, whole genome shotgun sequence.
ACCESSION   DASUTG010000216 DASUTG010000000
VERSION     DASUTG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220182"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VF089_04835"
     CDS             <1..793
                     /locus_tag="VF089_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173318.1"
                     /translation="SKNNLMSNRLAQELTKLASAYGAAEAEVVGAFFAVPRRKQDHLR
                     WLKAQGFKEYSAIKPILDALTQLYPNIDSGIARHDYTELTEKLADETKHARLVMDLLE
                     EISGKKIKPRDLTWLPQDRQLAKVRAGYSKTYAGLLHGSAKVTSKEMRRKDEELERAA
                     ITLTEGGGGALYQVCGKLKKRGIEEKIAAVFREILQDELEHKDSGGRSLATLVKDQGS
                     FQRAARIICEVSRQRLRMRNEQFGFPLSEAQMKTLDERARQSVGL"
     gene            826..1662
                     /locus_tag="VF089_04840"
     CDS             826..1662
                     /locus_tag="VF089_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6173319.1"
                     /translation="MPFPSLLRALELPPLNDTINDLAGIMPEPSYQDLEKRMKHFKAR
                     SGHSVVVIIIQSLEGEDPESYGEKAFHSLPLGEADLRKTILLIVARKEHAVYLQTGEE
                     LKELFPEPTAREKLEKHVDLYFSGMRPDLGIHSAVFFAFRTIRGEVKMDRATEFEKLE
                     EASTRGAEAGPIFAIFLAPFLAFFVGALWGICSTNYGVQTAVRLTTGAVLGGGTAKVV
                     AGLMELMGAVSAGLWYFILILSIALATFGSLTEFWMAGDWRGIPRVKDPVVRKPEDNI
                     GI"
     gene            1744..2727
                     /locus_tag="VF089_04845"
     CDS             1744..2727
                     /locus_tag="VF089_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173320.1"
                     /translation="MTSLMITDEQRAHPDIAQRLTISRRELLRLALAAGIFVGAGGAA
                     MGAATSSKAILTKPIPHTGESLPVIGLGTWQTFDVGAAQSERAPLREVLREFVRLGGT
                     VIDSSPMYGRSESVVGDLAAELKLEGKLFVATKVWTSGREAGTRQMEESFRRLRAKRI
                     DLMQIHNLVDWRTHLTTLRQWKTEGRIRYIGVTHYTASAYDQLARVLESEELDFVQLN
                     YSIAEREAEQRLLPLAGDRGVAVLVNRPFAQAQLFSKVRGKPLPPFAGEIGCATWAQF
                     FLKFVVSHPAVTCAIPATSKVQHLTDNMQGGMGQLPDAKIRQRMAKYVAEL"
     gene            2912..4600
                     /locus_tag="VF089_04850"
     CDS             2912..4600
                     /locus_tag="VF089_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX6173321.1"
                     /translation="MNDIFISYDSADRAVAQKFADALEGRGWSVWWDREIPLGKAFDQ
                     VIEEELNAARCVIVLWSNQSVRSRWVKTEAAAAADRDCLLPVLIEEIAIPFEFKRIQT
                     AMLMNWRGDDSDPEFNRLVKAIEQLAKEPARSIPSRPANQMNKPAGWRMPPGRWIIAG
                     VLVLLFIAGALMVRNFPRQADPPAQDQVSSGSGSIATPPTQPAGSPAPAPGPSTGAAK
                     AAAPDGTFSIKIGDKIADGVPGPGAGIIETPYGQDVYVFKAAARQRVYFRLLGRSSGM
                     DYINWKLTDADGTEVFNACLGCSEPGVRALTKGGAYTLTIGNEKNPATGTYQIQLIDV
                     PAPNQFSIKIGDQIKEDVPGPGAGIIESPGAQDIYTFEARPRQKVYFRAWEHSTGMSY
                     IKWQLTDSAGMEVFDTCLGCGEPGVQTLTSGGSYTLAVGHESNPATGTYRLQLYDVPS
                     SKQFSIKIGDRIGPGIPAAGAGTIESPGSEDVYVFSATPGQKVSFRMTERSKGIDYIN
                     WRLVDDNGMELFNSCLACGERGVQTLSKGGTYTLTIGNTRHPATGEYGFEIGAR"
BASE COUNT         1087 a         1341 c         1391 g          993 t
ORIGIN      
        1 atctaaaaac aatctaatgt caaatcgatt ggcgcaagag ctgactaagc tggcgtcggc
       61 ttatggcgcg gctgaagccg aggtcgtcgg cgcgtttttc gccgtgccga gacggaaaca
      121 agaccatctg cgctggctca aagcgcaagg gtttaaagaa tattccgcaa tcaagccgat
      181 cttggacgct ttgacccaac tctatcccaa catcgatagc ggcatcgctc gccacgacta
      241 taccgaattg acggaaaaac tcgcggacga gaccaagcac gcgcggctgg tcatggacct
      301 gctcgaggaa atcagcggca agaagatcaa accacgcgat ctcacgtggc tgccgcaaga
      361 ccggcaactg gccaaggtgc gcgccggcta ctcgaaaacc tatgccggac ttttacacgg
      421 atcggcgaag gtgacgtcaa aagagatgcg ccgcaaggac gaagaactgg agcgcgccgc
      481 gatcactttg accgagggcg gtggcggcgc attgtatcaa gtctgcggca agctgaagaa
      541 gcgcggcata gaagaaaaaa tcgccgcagt atttcgggag atcctacagg acgagctgga
      601 acacaaagat agcggtggcc gctccttggc taccctggtc aaagatcaag gctcattcca
      661 gcgcgccgca cgaattattt gcgaggtctc gcgccaaagg ctgcggatgc gcaacgaaca
      721 attcggcttt ccactaagcg aagcgcagat gaagacgctg gacgagcgcg cccggcaatc
      781 ggtcggcctg tgatgcttct gctgctttgg atcgcttgct taagcttgcc gtttccatct
      841 ctgttgcgcg ccctcgagct gcctcctctg aacgatacca tcaatgatct cgccggcatc
      901 atgcccgagc ccagctatca ggatctcgaa aagcgcatga agcatttcaa agcccgcagc
      961 ggccacagcg tggtcgtcat cattatacaa agtctcgaag gtgaagaccc cgaaagctac
     1021 ggtgaaaagg ctttccacag tttgcctctc ggagaagcgg atctgagaaa aacgattttg
     1081 ctgatcgtcg ctcgcaagga acatgcggtt tacttgcaaa ccggtgagga gcttaaagag
     1141 ttatttccag aacccacggc gcgagagaaa ttggagaagc atgtcgattt gtattttagc
     1201 gggatgcgtc cggacctggg aattcactcg gcggttttct tcgcattcag gaccatccgg
     1261 ggagaagtga agatggatcg cgccaccgag ttcgagaaac ttgaagaagc ctcgactcgg
     1321 ggtgccgagg cggggccgat ctttgcgatt ttcttagcgc cgtttttggc cttctttgtc
     1381 ggcgcgcttt ggggaatctg ctcaaccaat tatggcgttc aaactgccgt ccggctcacc
     1441 accggcgccg tgctcggtgg cggtaccgcc aaggtggtcg cgggtttgat ggaactaatg
     1501 ggcgcggtga gtgctggcct ttggtatttc attctcatcc taagcatagc tctcgcaact
     1561 ttcggcagcc tcaccgaatt ctggatggcg ggagactggc gcggcatacc aagagtgaag
     1621 gatcccgtcg tgcgcaaacc agaagataac atcggcattt gagtaggctg ccgcagtctc
     1681 gtcgatagaa gtgagactac acagttcaga ggttctctag tttcatccgt ggcgtacggt
     1741 acaatgactt cactcatgat aaccgacgag caaagggcgc atccggacat cgcgcagcgg
     1801 ctaaccattt cccggcgcga gttgctgcgg ctcgcgctcg ctgcggggat ctttgtgggc
     1861 gcgggaggtg cggccatggg cgcagccact tcatctaaag cgatactgac caaaccgatt
     1921 ccacacaccg gcgagtcact gcccgttatc ggtctcggca cctggcagac tttcgatgtc
     1981 ggggcagcgc aatccgagcg cgcgcccctg cgcgaggtat tgcgcgaatt tgtccggctg
     2041 ggcggcacgg tgatcgactc ctcgcccatg tacgggcgat ccgaaagcgt tgtcggagat
     2101 cttgccgccg aactgaagct cgaaggtaag ctctttgtcg ccaccaaagt ttggaccagc
     2161 ggccgcgagg ccggcacccg gcagatggaa gagtcctttc gccgcctgcg cgccaagcgc
     2221 atcgacctga tgcaaatcca caatctggtc gattggcgca cgcatttgac cactctgcgc
     2281 cagtggaaaa cggaaggaag aattcgctac atcggcgtga cccactacac cgccagcgct
     2341 tacgaccagc ttgcccgtgt gctcgaaagc gaggaactcg atttcgttca gctgaactat
     2401 tcgattgcag agcgcgaagc cgagcaacgg ctattgccgc tcgcaggcga ccgaggtgtc
     2461 gcagtgctgg ttaaccggcc gtttgcccaa gcgcaattgt ttagcaaagt cagaggcaaa
     2521 ccgctgccgc cctttgccgg ggagatcggg tgcgccactt gggcgcaatt ttttctcaag
     2581 ttcgttgtct cccacccggc ggtcacgtgc gcaataccgg cgacgagcaa agttcaacat
     2641 ttaaccgata atatgcaggg cggcatgggc cagcttcccg atgcaaagat tcgccagcgc
     2701 atggcgaagt atgtggccga attatgattc catgttgaat tcatgacggc ttcctatctt
     2761 catccaaccg ccatattctc gagctgactg cgccgtaaaa acgctttaca agagcacgac
     2821 tgaagagtag atttttccac cggcgcaggt gtcgatccga cccgccccta tcatatccat
     2881 caaatgtcag aatcattccg cagtatcctt catgaatgac atcttcatca gctacgacag
     2941 cgccgatcgg gcggttgcgc agaaatttgc cgacgctctg gaagggcgtg ggtggtcggt
     3001 gtggtgggac cgggagattc ctctaggtaa ggctttcgat caagtcatcg aagaggagtt
     3061 gaatgcggcg cgctgcgtca tcgtcttgtg gtcgaatcaa tcggtgcgct cgagatgggt
     3121 gaagaccgaa gcggcggcgg ccgccgatcg agattgtctc ttgcctgtgt tgattgagga
     3181 gatcgccatt ccgttcgagt tcaaacgcat tcaaacggcg atgctcatga actggcgcgg
     3241 cgatgattcc gatcccgagt tcaacaggct ggttaaagcc attgaacagc tggccaagga
     3301 gccggcacgc tcgattccgt ctcggcccgc aaatcagatg aacaagccgg ctggatggcg
     3361 aatgcccccg ggccgttgga tcattgccgg cgtgcttgtt ttattgttca ttgccggcgc
     3421 cctgatggtg agaaattttc ctcggcaagc cgacccgccg gcgcaggatc aagtctcatc
     3481 cggttccggc agcattgcga cgccgccgac acagccggcc gggtcacccg cgcccgcacc
     3541 gggaccttct accggcgcag caaaagcagc ggcaccggat ggcacctttt cgatcaagat
     3601 cggtgacaag attgccgacg gggtaccagg acctggcgcc ggcatcatcg agacgccgta
     3661 cggccaggat gtctatgtgt ttaaggccgc cgccaggcaa agagtttatt tccgcttgct
     3721 cggccgcagc agcggaatgg attatatcaa ttggaaattg accgatgcgg acggcaccga
     3781 ggtattcaac gcttgcttgg gatgttccga accgggagtg cgggcgctta ccaagggcgg
     3841 cgcctacacg ctgacaattg gcaacgagaa aaatccggcc acgggaactt atcagatcca
     3901 gctgatcgat gtcccggcgc caaatcaatt ctcgataaaa atcggcgatc agatcaagga
     3961 ggatgtaccg gggccgggag cggggatcat cgaaagcccc ggcgcccagg atatctacac
     4021 attcgaggca aggccgcgac aaaaagttta tttccgagct tgggagcaca gcaccggaat
     4081 gtcctatatc aaatggcagt tgaccgatag cgccggcatg gaggtgtttg acacatgcct
     4141 cggctgcggc gaacctggag ttcagaccct cacaagcggc ggcagctata cccttgccgt
     4201 cggtcatgag agtaatccgg ccactggcac ttatcgtttg caactctatg acgtcccttc
     4261 atcaaagcaa ttctcgatca agatcggcga tcggatcggg cccggcattc cagctgccgg
     4321 agcgggtacg attgagagtc ccggatcgga agacgtttat gtatttagcg ccacgccggg
     4381 ccaaaaggtg tcctttcgca tgaccgaacg cagcaaaggg atcgattaca tcaactggcg
     4441 cttagtcgat gacaacggca tggagctgtt taacagctgt cttgcttgcg gtgagcgcgg
     4501 cgtgcagacc ttgagcaaag gcggcactta cactctgacg atcggcaata cgcggcaccc
     4561 agccacaggt gagtacggtt tcgagattgg cgcccgttaa gtcgtcagct gcggcgcacc
     4621 gccggcgata aagaaatgat gatttgcaat cattccacca gcctcgacag cctgtgaatt
     4681 cagtttgccg ctcgtaggtg agcaaagctc gcgctagatc tgacccttcc acaaacaagc
     4741 atgttctaag tctcagcggc ggctgagctc tccggcaccg atcctccccg tatgtgtctt
     4801 gatgtcttat ac
//
LOCUS       DASUTG010000217         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_331182, whole genome shotgun sequence.
ACCESSION   DASUTG010000217 DASUTG010000000
VERSION     DASUTG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331182"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF089_04855"
     CDS             complement(<1..202)
                     /locus_tag="VF089_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX6173322.1"
                     /translation="MIVGRLAVPRRIHGVIFSDLGQASRFMALDWVQEALRQRLGFAP
                     YPATLNLQPSTPPDARLWEEIQR"
     gene            complement(212..982)
                     /locus_tag="VF089_04860"
     CDS             complement(212..982)
                     /locus_tag="VF089_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX6173323.1"
                     /translation="MSALSEELVRITEQGRSAVLATIVEAQGSDRVQAGAKCLVVDGK
                     ASTETIGDLRAIEQIVRESEAILSAEKSKLVAIEIPEIGRLQVFFEVMLAPPKLIVVG
                     AGHIAVPLVQIAKVLDFHVTVIDDRLLYANRERFPAADEVLVGDMAQMLTEMTITQSC
                     YIVLITRGHKYDEPCLRQILHSKAKYIGMIGSRRRIKACFQRFRDEEKIAEEVIERVY
                     APIGLDIATETPAEIALSILAEVVKVRRGGRAASLSGH"
     gene            complement(979..1374)
                     /locus_tag="VF089_04865"
     CDS             complement(979..1374)
                     /locus_tag="VF089_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6173324.1"
                     /translation="MSGQGTCFEDPVLEAKKKDSVYHQVKEFLDKGETVAVATIVSAK
                     GSTPREVGAKMVVTAWGEILGTIGGGCGEADVKREAIEVIRTRKPRMVRVDLLDDISS
                     DSPAVCGGIMNIFVDPWWEERDGESAAKE"
     gene            complement(1396..2607)
                     /locus_tag="VF089_04870"
     CDS             complement(1396..2607)
                     /locus_tag="VF089_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6173325.1"
                     /translation="MEITPESVAAAVNSFAAVRGHGTVANLLAFGRALKQLGVKVSLS
                     QVIDTSRSLNLVDIAEKEDFRALLRSNLISQVEDFPIFDTVFDCFWREQNYERVPMET
                     LEIEGTPTESQAQEGGEEEGLDEALAEAPAKENVELENLDEFSIPTYSAQELLNRKDF
                     SEMSVEESRAIARAILLIATKIATQISRRKKHARKGATVDPRWTLRKNIKYGGDVVEM
                     VKRKRRIKKTKVVLLCDVSGSMDCYSRFLIQFMYGLQNELWGVETFVFSTSLTRITHL
                     IRTKDIANALEQISGSILGWSGGTNIGRSLQTFNRNFAPAMVTSRTIVVIISDGWDRG
                     DVSLLERQMQDLKRRCKKVIWLNPLLASENYEPLCKGMQAALPYIDLFLSVHNVNSLV
                     ALGRTLQKMVA"
     gene            complement(2613..3533)
                     /locus_tag="VF089_04875"
     CDS             complement(2613..3533)
                     /locus_tag="VF089_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6173326.1"
                     /translation="MDPKQLTIDDVQEILLKEEYVCDRSLATVVYLSLAMGKPLLLEG
                     EAGVGKTEIAKVLAKVLNAKLIRLQCYEGLDANTALYEWNYPKQMLRIKLEEVQRGDK
                     KAVETEIFNQDYLLKRPLLEAIISEEENPPVLLIDEIDRADVEFEAFLLEVLSDFQIT
                     IPEIGTVQAKQRPYVFLTSNRTREIHDALKRRCLYHWIEYPTFEKELEIISTKFPEVA
                     PAMAKQICAFMQRVREMNFYKRPGVAETLDWATALIALNRKGLDDKTVVETMGCVFKY
                     REDLHHLKEQIEGKHLNLDALLRSSPELMS"
     gene            complement(3571..>4407)
                     /gene="yqeB"
                     /locus_tag="VF089_04880"
     CDS             complement(3571..>4407)
                     /gene="yqeB"
                     /locus_tag="VF089_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HEX6173327.1"
                     /translation="FVMLWSGMTVAISELIVLIRGGGEMATGIAHRLHRCHMRVLITE
                     IAIPTAVRRNVAFAEAVYEGVQTVEGVKAVRVRSCDEAYAAWEQNQIPVFVDAEASIR
                     KILKPVVVVDAIMAKKNGATKLTDAPVVIAVGPGFRAGVDAHAVVESNRGHHLGRVIW
                     DGAAESYTGIPASVDGYTASRVLRVPQSGIFNALRAIGDVVAAGEPVATVQGELIKAE
                     ISGQIRGLLRNGIQVQQGIKAGDIDPRGERGYCDSISDKSRAIAGGVLEAVLHSLRNL
                     NS"
BASE COUNT         1010 a         1312 c         1042 g         1043 t
ORIGIN      
        1 ttctctggat ttcctcccac agccgcgcat ccggcggcgt tgatggctgc aaattgagag
       61 tagccgggta cggcgcaaaa cctaaacgtt gccgaagcgc ttcctgcacc cagtctagcg
      121 ccatgaatct ggatgcctgg cccaagtccg aaaaaatcac accgtgaatt ctgcgcggca
      181 ctgccagccg tccaacgatc aagctccggt ctcaatggcc tgagagcgat gccgccctgc
      241 cgccgcgacg aactttgacc acctcagcca gaatcgacaa ggctatctcg gccggcgtct
      301 cagtggcgat atccagacct atcggcgcat agacccgctc gatgacttcc tcggcgattt
      361 tctcctcgtc ccggaatctt tgaaagcagg ccttgattcg gcgccggcta ccgatcatac
      421 cgatgtattt cgctttactg tgaaggatct ggcgtagaca aggttcatcg tatttatgcc
      481 cgcgggtaat caggacgata taacaggact gagtaatcgt catctccgtc agcatttgcg
      541 ccatatcgcc caccagcact tcatcggccg ctggaaacct ttcgcgattg gcataaagga
      601 ggcgatcgtc aatgacagtg acatggaaat ctagaacctt ggcgatttga accagaggca
      661 ctgcgatgtg accggcgccg acaacaatca gtttgggcgg cgccaacatg acttcaaaaa
      721 acacctgaag tcttccgatt tcgggtattt caatcgcaac aagcttggac ttctccgcgc
      781 ttaaaatcgc ctcgctctca cgtacaatct gctcaatcgc gcgtagatcg ccgattgtct
      841 cggtgctggc tttgccgtcc accaccaggc atttagcgcc ggcttggaca cgatcgcttc
      901 cctgcgcctc gacgattgtc gcaagaacag cggatcggcc ttgctcggtg atccgcacca
      961 attcttcaga aagtgcgctc attccttggc cgctgactcc ccatcgcgct cttcccacca
     1021 ggggtcgaca aagatattca tgatgcctcc gcaaacagcc gggctgtcgg acgatatgtc
     1081 atcgagcaga tccacacgca ccattcttgg tttgcgtgtc cggatgactt cgatagcttc
     1141 gcgcttgacg tcggcctcgc cgcacccacc gccgatagtc ccgagaattt ctccccatgc
     1201 cgtgaccacc atcttcgcgc caacttctcg aggagtcgag cccttagccg aaacgatagt
     1261 cgccacggcc acagtttcgc ctttgtcgag aaactctttt acctggtgat aaaccgagtc
     1321 tttcttcttc gcttccaaaa cgggatcttc aaagcacgtt ccttgacctg acatgatttt
     1381 cctccgtgca aactgctaag ccaccatctt ctgtaaagtg cgtcccaacg cgaccagact
     1441 gttgacatta tgaaccgaca agaacagatc gatgtacggc aaggctgcct gcattccctt
     1501 gcacaacggc tcataatttt cactggcaag caacgggttg agccagatca ctttcttgca
     1561 tcgacgcttt aggtcctgca tctgccgctc tagcagacta acgtcgccac ggtcccaacc
     1621 gtcactgata ataacgacga tggttcgaga ggtcaccatc gccggagcaa aattccgatt
     1681 gaaagtttgc agagaacggc caatgttagt gccgccagac cagcccagaa tgctgccgga
     1741 gatctgctcc agtgcgttgg caatatcttt ggtgcgaatc aaatgggtga ttcgggtgag
     1801 cgaggtgcta aaaacaaacg tctcaacacc ccatagctca ttctgcaacc cgtacatgaa
     1861 ctgaattaaa aatcgactgt aacaatccat tgagccgctg acatcacaaa gcagcacgac
     1921 tttggttttt ttgatccggc gcttgcgttt gaccatctcg acgacatcgc cgccgtattt
     1981 gatatttttt cgcaaggtcc agcgcgggtc caccgttgcc cctttgcgcg catgtttctt
     2041 gcggcggctg atctgggtgg caattttcgt agcgatcagc aaaatcgcgc gggcgatcgc
     2101 gcgactctct tcgacactca tttcgctaaa atctttgcga ttgagcaact cttgagcgct
     2161 gtaggtcgga atcgaaaact cgtccagatt ctccagctcg acgttctctt tcgccggtgc
     2221 ttcagccaag gcttcatcca acccctcctc ctcgccgcct tcttgcgcct gagattcggt
     2281 aggcgttcct tcgatctcga gcgtctccat cggcactcgc tcgtagttct gttcacgcca
     2341 gaagcaatcg aacaccgtgt cgaagatagg aaaatcctcc acttgagata tcaaattgga
     2401 gcgcaggaga gcgcgaaaat cctccttctc ggcgatatcc acgagattga gcgagcggga
     2461 cgtgtcgatg acttggctca gacttacctt gacgcctagt tgcttcagag cgcgcccgaa
     2521 ggcgagtaaa ttggccaccg tgccgtgccc gcgaaccgct gcaaaactgt tgaccgcagc
     2581 tgcgacgctt tccggtgtga tttccatagc cgctaactca tcaactcggg ggaagaccta
     2641 agcaacgcat ccaaattgag atgtttgcct tcgatttgct cttttagatg atgtagatct
     2701 tcccgatatt tgaatacaca acccatcgtt tcaaccacgg tcttatcgtc caaccccttt
     2761 cggtttagtg caataagagc tgtcgcccaa tccaacgttt ccgcgacacc cggccgctta
     2821 taaaaattca tctcccgaac ccgctgcata aaagcgcaaa tctgcttggc catcgccggc
     2881 gcgacttctg ggaacttggt cgaaatgatc tccagctcct tttcaaaagt cggatactcg
     2941 atccaatgat aaaggcaccg tcgcttgagg gcatcatgaa tttcgcgggt ccgattggat
     3001 gtcaaaaaga cgtacggacg ttgcttggct tgcaccgtgc cgatctcggg gatggtgatc
     3061 tggaagtctg atagtacttc cagcagaaag gcttcaaact caacgtcagc gcggtcgatt
     3121 tcatcgataa gcagtaccgg cggattctcc tcttcactga tgatagcttc caacaaaggg
     3181 cgtttgagca agtaatcctg gttgaagatc tcggtttcga ccgccttttt atcgcctctt
     3241 tgaacttcct caagtttgat gcgcaacatc tgctttggat aattccactc gtaaagagcc
     3301 gtgttggcat ccaagccttc gtagcactgc agccggatca gtttggcgtt caacactttc
     3361 gcaagcacct tggcaatttc ggttttaccg actccggcct ctccttcgag cagcaacggt
     3421 ttacccattg ccaatgaaag atacaccacg gtagccaatg agcggtcgca aacatattcc
     3481 tctttgagca gaatttcctg aacgtcgtcg atcgtcagct gttttggatc cacgccagcc
     3541 tcaccattga gagttttgaa tttgaaatcg tcatgaattt aagttcctga gagaatgaag
     3601 aaccgcttcc aacacgccgc cggcgattgc ccgcgactta tcggaaatcg aatcacaata
     3661 acccctttca ccacgcggat caatgtcgcc agctttgatg ccttgctgta cctgaatgcc
     3721 gttgcggagt aggccgcgga tctgacctga gatctccgct ttgatcaatt caccctgcac
     3781 ggttgcgacc ggttcgcccg ccgcgaccac atctccgata gcacggagcg cattaaatat
     3841 cccgctctgc ggcacccgca gaactctaga cgctgtgtag ccatccactg aagcgggtat
     3901 gccagtgtag gactcggcgg ccccgtccca gatcacacgg cctaaatgat gaccccgatt
     3961 actctcgacc actgcatgcg catccacacc agccctgaag cccggaccca cagcgatcac
     4021 aaccggcgcg tcagttagtt ttgtagcccc attcttctta gccatgatgg cgtcaacaac
     4081 gacgacgggc tttagaattt ttctgatgga tgcctcggca tcgacaaaaa ccggtatctg
     4141 gttttgctcc caagcggcat atgcttcatc acaggaacgg acccggacag ccttgacccc
     4201 ttccacggtt tgaacgcctt cgtaaactgc ttcagcaaac gcgacattgc gccgcaccgc
     4261 cgtgggaatg gcgatttcag tgatcaacac ccgcatatgg cagcgatgca gccggtgcgc
     4321 gatgcccgta gccatctcgc cgccaccacg gatcagtaca atcaactcag aaatcgccac
     4381 ggtcatacct gaccataaca taacaaa
//
LOCUS       DASUTG010000218         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1072921, whole genome shotgun sequence.
ACCESSION   DASUTG010000218 DASUTG010000000
VERSION     DASUTG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072921"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF089_04885"
     CDS             complement(<1..544)
                     /locus_tag="VF089_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173328.1"
                     /translation="MDLAIIGCTSSTTNYILLGNKQTRTVEDLRGKLIGVTGAGTYSE
                     FAMKAFLKKNNINPDKDVVLRAIGGTVLRAAAIEKGIIAAAPFSTEDGVRLIKAGYSV
                     ISNMSESLGIPQNILVTRNDMLEKFPETSKRMLKAYIQGINLAKFNKPEAIKSGYEAG
                     LQGEPDIVSAAWVLYAPGLTS"
     gene            1098..1277
                     /locus_tag="VF089_04890"
     CDS             1098..1277
                     /locus_tag="VF089_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173329.1"
                     /translation="MNKIRRHVTFYDVTLYQSGMAGIESLGYFVFNLDIRELLVAEAL
                     LFNVKTMRLQVPDPR"
     gene            1567..>2197
                     /locus_tag="VF089_04895"
     CDS             1567..>2197
                     /locus_tag="VF089_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173330.1"
                     /translation="MKSSFKILDSDMHLMEPVDLWERYIDAKYKADAPRGLTSDNVRD
                     LRMAHPDGRYWGLPASHNRNSSHHRGHNFKKNQDIYRSHAERGWTADVQLEAMDIEGI
                     DVAVLYPTRGLQVLSEPRMEPKFAAALARAYNDGFTIFVKSTRPGSSARECFRLSIST
                     KPSPKQSAAPRNSGFAPFFCARASWRAEIGMTNTSSRSGRPWKNTNCLSA"
BASE COUNT          491 a          616 c          588 g          502 t
ORIGIN      
        1 ccgaagtaag gccgggcgca tagagaaccc aggcggcgct gacgatgtcc ggctcgccct
       61 gcaagcctgc ctcgtacccc gacttgatcg cttccggctt gttgaacttg gcgagattga
      121 ttccttgtat gtaggccttg agcatacgct tgctggtctc gggaaacttc tccagcatgt
      181 cattgcgggt taccaggatg ttctgcggaa tacctaacga ctcgctcatg ttgctgatca
      241 cggaatagcc cgctttgatg agccgaacac cgtcttctgt ggaaaacggc gccgcggcga
      301 tgatcccctt ttcgattgcc gccgcgcgca aaaccgtgcc gccgatcgcg cgcagcacca
      361 cgtctttgtc ggggttgata ttgttctttt tcaaaaacgc tttcatggca aactcggaat
      421 acgttcccgc gccggttacg ccaatgagct tgcctctaag atcttcaacg gtacgggtct
      481 gtttgttgcc gagcagaata taattagtcg tgctcgaggt gcatccgatg atagccaagt
      541 ccaagccgcg cgccttactg cgaaaaactg cctgtgcgcc accatggcgg caaagtccac
      601 ttcaccggaa accagagcgg cgatcgccgc cggacctccg cgaataagga taggttcgac
      661 ttggagccct tgctgcttga agaagccgcg cgcgagcgct gtccggaacg gaatctcgct
      721 gttccaggcc aggctgacaa aaacgatccg cacgttcttc ttttcttgtg cgagagttgc
      781 caacggctgt gccagcataa aagagacaag cactcccaag cagagcggac cgaaatcgtg
      841 agagagagtt ggcagcttca ttcagtctcc ttcccggtcc gaaggtgctg gcttgcgcga
      901 tttcaactga ttgaacgggc tttaactttt gaacacgtta agcaggttta tcttctggcg
      961 tatactgacg tgcgaccttg cttgtcaaaa ctgattacct catagggctg cgggttcggc
     1021 ccctccatcc ctggagatcc gatcggcatg cccgctacag cgaggcccgc cagcggtggt
     1081 ttctccttaa gcagacgatg aataagatcc gccggcacgt gaccttctat gacgtaacct
     1141 tgtaccagag cggtatggca ggaatagagt ctctcgggta ctttgttttt aatcttgata
     1201 tccgcgagtt gcttgttgcc gaggctcttc ttttcaacgt taaaaccatg cgattgcagg
     1261 tgcccgaccc acgctgaaca gcatcctcag ccccaaccct gataaaccat taccgatgta
     1321 gccgcaggtt ttgattgcgc atcgcttgcc gacccgttca tgagcaggaa gagaagcata
     1381 ctgacagacc acaagcgaaa aaagttagtt aaaaacatca tcatcttctc ccgatcaata
     1441 tttgtgacgc ctcactttac cagatcacct gaatgcggca aagggtttta cggatgactc
     1501 acgttgacac tgaagccggc ggtgcgttat cggtaaggta gcccgtggct caatcaaagg
     1561 tgatttatga aaagcagttt caaaattctc gacagcgata tgcatttaat ggagccggtg
     1621 gatctgtggg agcgctatat cgatgcaaag tacaaagccg atgcgccgcg cggcctgacc
     1681 agcgacaacg ttcgcgatct gcgcatggcg catccggacg gccgctattg gggattgccg
     1741 gcaagccaca atcgtaactc atcgcaccat cgaggtcata atttcaaaaa gaatcaggat
     1801 atctatagat cgcatgccga gcgcggctgg actgccgacg tccagctcga ggcgatggac
     1861 atcgaaggca tcgatgtcgc ggtgctctac cccacccgcg gcctgcaggt cttgagcgag
     1921 ccccgaatgg aaccgaagtt cgccgccgcc ctggcgcggg cttataacga tggctttacg
     1981 atttttgtaa aatcaacccg gcccggctcc tcggcgcggg aatgctttcg cctttcgata
     2041 tcgacgaagc cgtcgccgaa gcaaagcgct gcgccacgga actcgggttt cgcgccgttt
     2101 ttctgcgctc gtgcatcatg gagggccgaa attggtatga cgaatacttc gagccgctct
     2161 gggcgacctt ggaagaacac caactgccta tcggctt
//
LOCUS       DASUTG010000219         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_776640, whole genome shotgun sequence.
ACCESSION   DASUTG010000219 DASUTG010000000
VERSION     DASUTG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776640"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /gene="mltG"
                     /locus_tag="VF089_04900"
     CDS             complement(<1..972)
                     /gene="mltG"
                     /locus_tag="VF089_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6173331.1"
                     /translation="MKWFLFTVLALVVTGLLFGGFLVAHIFTASSGNSQPVEVRVEQG
                     EPFAAVVRKLRAQRVIPNDKLFSLWARLTRSDKKVQWGVYRFDPSLAPREVLHRLVTG
                     KRVFLMVTIPEGLTIKEIAELLAKMQIADREKFLAAAADAELLASLGLEDKGLEGYLF
                     PNTYQFTPMTSEKEIIVTMAEQFRKASEPIWDQLDKGHKLSRHEIITLASIIEKETGI
                     DAERPVVSAVFHNRLKRNMALQSDPTVIYGLKEFDGNLTRKDLKDQSPYNTYRIPALP
                     PGPICNPSLASIKAALQPADVPYLFFVSKNDGTHIFSQDLESHNQAVR"
     gene            complement(1021..1548)
                     /locus_tag="VF089_04905"
     CDS             complement(1021..1548)
                     /locus_tag="VF089_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173332.1"
                     /translation="MNAIRQSFWQLGIYDATVAGYVADVLTDFARTDSLYRLRSRAGR
                     KIESVVEMLAHESELPASENQLLRERETRKYVGDYALFMSGIFRTYVEGRGFLDYYIK
                     EGSRSYWTVSELDVSLYRTGFILFQELSTKFEYYSGALDYTRKAYFAAQPGEDPFAGF
                     LRQVDGWMKVNLTEN"
     gene            1820..2338
                     /locus_tag="VF089_04910"
     CDS             1820..2338
                     /locus_tag="VF089_04910"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX6173333.1"
                     /translation="MGRDLKEAFFRLDGAWASFELGSIRRRDDYLKPFRVLRCKIDDQ
                     VDFQNPEAPRSTTEASVLIEIFGEQELVAASGRGPVHAVDNAMRKILEKHFPQLAEVR
                     LEEFDVRLLPHQLPQDDDETGAGALVRVLGIFSDGEDRWGTVGVADDILQACVECIID
                     GLEWKLRGEQKH"
     gene            complement(2421..2663)
                     /locus_tag="VF089_04915"
     CDS             complement(2421..2663)
                     /locus_tag="VF089_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173334.1"
                     /translation="MEQFDIQHAITDVSGVSVKPDKSGAGLLMGNKPSMQPEAVFRRK
                     ENLFESQSYMTWIATRTMIGMENESVLDPATAEQGC"
BASE COUNT          661 a          705 c          704 g          645 t
ORIGIN      
        1 gcgcaccgcc tgattatgcg actccaaatc ttgtgaaaat atatgagtgc cgtcattctt
       61 tgaaacaaag aacagatagg gtacatcagc cggctgcaga gcggctttga tggatgcaag
      121 gctcgggttg cagatcggcc ctggtggcaa cgccggaatc cggtaggtgt tataagggct
      181 ttgatcttta aggtctttgc gggttagatt gccatcgaat tcctttaaac cataaatcac
      241 cgtcggatcg ctttgtaagg ccatatttcg tttgagccga ttgtgaaaaa ccgccgaaac
      301 caccggccgt tcagcgtcga tgcctgtttc cttctcgatg atggacgcta gagtgatgat
      361 ctcgtggcgg ctcaacttgt gacctttatc gagctggtcc caaatgggtt cagacgcttt
      421 gcggaactgc tccgccatag tcacgataat ctccttttca gaagtcatcg gcgtaaactg
      481 ataggtgttc ggaaataagt agccctccag ccctttgtcc tctaatccaa ggcttgccaa
      541 taactcggcg tccgcagcag ccgcaagaaa cttctcccga tccgcgatct gcatttttgc
      601 gagcagctcg gcgatttcct tgatcgttaa tccttctgga attgtcacca ttaagaagac
      661 ccgcttgccg gtaactagcc gatgtagaac ttctcgcggc gccaaggaag gatcgaaacg
      721 ataaacaccc cattgaacct tcttatccga gcgggttaac cgcgcccaca atgagaatag
      781 cttgtcattc gggatgactc gctgcgcgcg caatttgcgg acaacagccg caaatggctc
      841 tccctgctca acccgcactt cgactggctg agagttgcct gagctggcgg taaagatatg
      901 tgctaccagg aacccgccga acagcaagcc agtaacaacc agagcaagta ccgtaaaaag
      961 aaaccacttc acgattcggc taaagaactg cgaggcgggt caggatagct ggcgttgcag
     1021 tcaattttcg gtgaggttga ccttcatcca accgtccacc tgccgcagaa aaccagcgaa
     1081 aggatcctct ccgggttggg cggcaaagta ggccttgcgc gtataatcca gcgcgccgga
     1141 atagtattca aattttgttg aaagttcttg gaacagaatg aatcccgtgc ggtagagcga
     1201 tacgtctaac tcagaaacag tccaatacga acggctacct tctttgatgt aataatccaa
     1261 gaaaccgcgg ccttccacat aagtccgaaa aattccgctc atgaagagag cataatcgcc
     1321 gacgtatttc cgggtttctc gctcgcgtaa gagttggttt tcactagccg ggagctcgga
     1381 ttcgtgcgcc agcatctcaa caacactctc aatcttccgg ccggcccgcg accggaggcg
     1441 atagagacta tcggtgcgag caaaatcggt caacacatca gcgacgtagc cggcgactgt
     1501 tgcgtcataa atgccgagtt gccagaaact ctggcgaatg gcattcataa agaattcccg
     1561 gagtttgttg tctaagcttt gcttttccac tctaatggct gatgatacca aacttactcg
     1621 ggcttgaaaa gaatatcgtc gcggcccatg agcctgcttc tacgtaccgc gacccccgcg
     1681 cctcataaac cggaaagcta acacctgatg cggcagacac cacaaattga ccatggtaaa
     1741 ttttggattt tgtgtttaat tttgaggtga gtagacgggg agagtcacgc gctatgccac
     1801 tttcaaggag gaatcagcga tggggcggga tttaaaagag gccttttttc gtctcgacgg
     1861 cgcttgggct tcgttcgagc ttggatcgat tcgccgccgt gacgactatc tcaaaccttt
     1921 tcgagtgctc cgctgtaaaa tcgacgatca ggtcgacttc cagaaccccg aagcgccccg
     1981 gagtacaacc gaggcttccg ttttgatcga aatcttcggc gaacaggagt tggtcgccgc
     2041 gagcgggcgc gggcctgttc atgcagttga taacgccatg cgcaaaatcc tcgaaaaaca
     2101 ttttccgcaa ctagcggaag tccggctgga agagttcgat gttcggctac tgccgcacca
     2161 gctaccgcaa gacgacgatg aaactggcgc cggcgcgctg gttcgtgtcc taggcatctt
     2221 ttccgatgga gaggatcgtt ggggaaccgt cggggtcgcg gacgatatcc tccaggcatg
     2281 cgttgagtgc atcatcgacg gtctggaatg gaagctcaga ggcgagcaga aacattgaaa
     2341 tgagctactg ccgcggatcg cctgaacgcg ggtatttcat gtcttctttg cgactgaaac
     2401 ttttgtcgtt gatagtgcta tcaacaaccc tgttcggcgg ttgcaggatc gaggacagat
     2461 tcattttcca tcccgatcat ggtacgggtc gcgatccaag tcatatagga ctgagattcg
     2521 aagaggtttt ctttacgacg aaagacggcc tccggttgca tggatggttt attccccatc
     2581 aagaggcccg cgccactctt gtctggtttc acggaaacgc cggaaacatc agtgatcgca
     2641 tgctgaatat caaattgctc cacgacagaa tcggaactaa tctattcatt ttcgactacc
     2701 gcggctatgg tcgga
//
LOCUS       DASUTG010000220         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_776686, whole genome shotgun sequence.
ACCESSION   DASUTG010000220 DASUTG010000000
VERSION     DASUTG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776686"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF089_04920"
     CDS             <1..297
                     /locus_tag="VF089_04920"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6173335.1"
                     /translation="IDGYLNGSPTSAKAIFRQIHEGKRLNLKEVFLREWDMALNFCAR
                     SDFREGVRARLIDKDQKPQWRPPVLAQVQDAEIERLFSKQHGQPNLLSQRFAEL"
     gene            434..1438
                     /locus_tag="VF089_04925"
     CDS             434..1438
                     /locus_tag="VF089_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173336.1"
                     /translation="MWISALLFGWLVFGATVTAFSQDRNLIEAAKKEGGKVVVYGSME
                     TFTADAIKAAFEKKTGLEMEYWRGSSTEVMDRSLSEHRVGKPIFDVVATTGDHMHLMA
                     KEGVFVKYESPSFKGFAKEAIDPTLGARYRNVLYGVIYNKSGIKASEAPKTLEDVVKP
                     EYRGKLVMPHPVNHTLTTQWLASLDKIMPKARAEKFIRDLAAAKPFFVESIVPAADRV
                     GTGETPIGITFVRFVLTYNDRGANLDYVRDYTMLGDGQYISLAAKAPHPNAGKAFIDF
                     FLDKESMTIQAKTGEFVNRKGIYPPLADADKIKFVQMYQFSKEDYQSKKKEYQKIFLQ
                     "
     gene            1553..>2396
                     /locus_tag="VF089_04930"
     CDS             1553..>2396
                     /locus_tag="VF089_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173337.1"
                     /translation="MAELVIDADGHIMEQHKDLFDHIKGNFGEMNWHSSWPLFDADGW
                     QRGLARKGKREDPDAEAWIRFQNEHGIDMAVLYPTSALALGMIQLPAWASALAQGYND
                     WLYHRFTSQTPRLKGVALLAPQDPKAAAAELRRCVKELGFCAGLLPSVTNNRTPLYGS
                     PVFHEIYDEAQRLNVPLTIHGGVSQNLGLDRVASFLEAHMLEHPVGLFLQITNMVFQG
                     VFQEFPKLRVAYLEAGAGWVPFMMDRMEEDYEKFAPRLAPQLKSPPSHFFKSGNIFVS
                     MEVGE"
BASE COUNT          587 a          658 c          635 g          516 t
ORIGIN      
        1 atcgatggtt atcttaacgg ctcgccaaca tcggcaaagg ctatttttcg acagatacat
       61 gagggtaaaa ggctcaattt gaaagaagtc ttcctgcgcg aatgggatat ggctttgaat
      121 ttttgtgcac gctctgattt ccgcgaaggt gtcagggcgc gtctgatcga taaagaccag
      181 aagccgcaat ggagaccgcc ggttctcgcc caagtgcagg acgccgagat cgagcgcctc
      241 ttttctaaac agcatggcca gcctaatctt ctgtcgcaaa gatttgccga actctgatcg
      301 ctcacttcaa aacggcatca cagaaaagca tcgtttcagc tcccaaccta actgtcttga
      361 cggaatcaat ccggctgcaa tatataactc agcagaaaaa gattcctgac aacaaccgta
      421 aggagggtta acgatgtgga tttcggccct gctatttggc tggttggtgt ttggcgcgac
      481 cgtaacggcc ttctcgcaag atcgtaattt gatcgaagcg gcaaaaaagg aaggtggcaa
      541 ggtagtcgtc tacggctcca tggagacttt caccgccgac gcgatcaaag ctgcttttga
      601 aaaaaagacg gggctggaga tggaatattg gcgcgggtct tcaaccgaag taatggaccg
      661 gtctctcagc gagcaccgtg tcggcaaacc gatttttgac gttgtggcaa cgaccggcga
      721 tcacatgcac ctgatggcga aagagggtgt cttcgttaaa tacgaatcgc cgtcgtttaa
      781 aggatttgct aaggaagcga tcgatcccac cctaggggca cgctaccgaa acgttctgta
      841 tggtgtcatc tacaacaaga gcggcatcaa agcttccgaa gcacccaaga cgctggaaga
      901 tgtcgtcaaa ccggagtatc gcggcaaact cgtcatgccg cacccagtca accacacgct
      961 gaccacccaa tggctcgcca gccttgacaa gattatgccc aaagcgcggg cggagaaatt
     1021 catccgcgat cttgccgcgg cgaaaccctt ttttgttgag tccatcgtcc ccgccgccga
     1081 tcgggtcggc accggcgaaa cgcccatcgg tattactttc gtccgcttcg tgctgaccta
     1141 taacgaccgg ggcgccaatt tggattacgt ccgcgactac accatgctgg gtgacggaca
     1201 atatatcagc ctagcggcca aagctcccca tcccaacgcc ggcaaggcat ttatcgactt
     1261 cttcctcgac aaagaaagca tgacgatcca ggccaaaacc ggcgagttcg tcaaccgcaa
     1321 aggcatctat ccgccgctcg ccgatgcaga taaaattaaa tttgtacaga tgtaccagtt
     1381 cagtaaagaa gattaccaaa gcaaaaagaa agaataccaa aagatttttt tgcagtgagc
     1441 gctcgaccag aagcttgcgc acagatattc agaagccagt ttcgaccggg tcttcatccg
     1501 gtcggcacga cggcagcggc agtttgaaaa catcaaatca ggaggcacca caatggcaga
     1561 gttggtcatc gatgcagacg gacacatcat ggagcagcac aaggatcttt tcgatcacat
     1621 caaagggaat ttcggcgaga tgaactggca ctccagttgg ccgctgttcg atgccgatgg
     1681 ctggcagcgt ggcctggcac gcaaaggcaa gcgcgaagat cctgatgccg aggcctggat
     1741 tcgcttccaa aatgagcatg gcatcgatat ggcggtcctc tacccgacat cggcgctggc
     1801 gcttggtatg atccaattgc cggcatgggc ttcggcgcta gcccagggat ataacgattg
     1861 gctgtatcac cggtttacca gccaaacacc gcgcctgaaa ggtgtcgcgc tgcttgctcc
     1921 gcaagatccc aaagccgccg ccgctgagtt gcgtcgctgc gtcaaagaat taggtttttg
     1981 cgccggtctg ttgccctccg tcaccaacaa tcgcacgccg ctctacggct cgccggtgtt
     2041 ccacgagatc tacgatgagg cccagcgtct caatgtgccg cttaccattc acggcggcgt
     2101 cagccaaaat ctcggcctgg atcgcgttgc cagctttttg gaagcgcata tgctcgagca
     2161 tcctgtgggc ctgtttctgc aaattaccaa tatggttttc cagggggtgt tccaggaatt
     2221 tcccaagctt cgtgtcgcct atctcgaagc cggtgccggc tgggtgccgt tcatgatgga
     2281 ccgcatggag gaggactacg agaagttcgc cccgcgcctc gcgccccagc tgaaaagtcc
     2341 gcccagccac ttcttcaagt cggggaacat cttcgtcagc atggaggtcg gtgagc
//
LOCUS       DASUTG010000221         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_629012, whole genome shotgun sequence.
ACCESSION   DASUTG010000221 DASUTG010000000
VERSION     DASUTG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629012"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="VF089_04935"
     CDS             <1..1998
                     /locus_tag="VF089_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173338.1"
                     /translation="YEVRVSGGYGDISQYSITQSPGQADHALQYLFETDSSARSYSPY
                     KSLKDFSNQGKIFYEPLRKRMKPTAPRYVNINIVGGKKHTLIPQTQSLEANRMYYVRI
                     NIGTLSPDTAVENPKPFPAQLLPIAKGGHWLETVVVSDDFKFEKHRYRFFLPNRGASW
                     VCTCPPHDPHTCDDRTRENYLYIPTRPIKEIPEARLRIGIYYGNNLIQSQLLTAVIGE
                     TERESRGYSSKIDYTLTANVNNLASFTPRTLNILTNQNADGTHRIVINGELDDAVTFN
                     LTEGKVGNAIGAVRHKLLELHMRRYGGQLGSKPQFENLYDNNNAKSKVDFLDDLKQLA
                     PCGRLLWELLLTDQPERRRKLRNEILRNPSTIQVSRVQGSNFVFPWGVVYDIPLESDS
                     EQHRPCRLLDEWEQAGVLIDAVQPHCPYDNSHGKNTICPFGFWGFKHIIEQPPSMPDG
                     RDLQIAINVANRPIELLVALSLDLDQSLSKTHIEALETDLVNIMKVVPCRSRRNIEAA
                     LAQPKLELVYFYCHGERQAVSGSNVPVPFLEIGHQEKLTTGDIISWSDSDWDKHHWLD
                     TSPLVFINGCHTAELTPQILVNFVDAFTGVFAAGVIGTEIMLHQRVANEAGQIFFDYF
                     KNPKCSVGEALQQVRRSLLLKGNLLGLAYTAYCSADLQLST"
     gene            2049..2324
                     /locus_tag="VF089_04940"
     CDS             2049..2324
                     /locus_tag="VF089_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173339.1"
                     /translation="MSFTIIGSACFGFVIGWITLRTLRRKEGAAALSDIATVIGAVGG
                     AGVTALFKSESLFAGYSMGLFIGFVAYLIVGLIIGGRDDFKGWMGTP"
     gene            2746..>4693
                     /locus_tag="VF089_04945"
     CDS             2746..>4693
                     /locus_tag="VF089_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173340.1"
                     /translation="MIWTRARDGFAIGVLNDVLSSPDFSHNQPLDVKCSACGHEYSGE
                     LWLIVDVLARRDLYRRLVDGTLHFLRCPLELTVAVAVEVPFLLFRADSDLKLIFCVPS
                     GTQPEEIEPQAKMCLDLLQARMGDAWDESWPETAMGRALDIGLHSMVSDEWSDLRSYG
                     EHDPFLEKLISFPEVKDLDEKRQLLFHEPDLLGRSAINTLGFFSQSLMEQGKPIKAHD
                     VESQRRLLDRCTEIGIDAAFAEFREQYPPPLSVGTVDAETRAMLEDAIAIPTGLGDVL
                     RKIELCENLLTRLDPHQNSFFRVMVQNTLARRYYESSAGDRRANVEHAIELLTSILSG
                     LNPQQDPEAWASAIVSLSNAYQLRIEGTPAANFRKAIELCEQALKHISEKEYPDSYCP
                     LMDALASAYVDDPGGDRVDNIERTIAAHEKILQIEKKPDARTISLHNLARAYGLRIRG
                     SREVNVERSIELYEAALAGISAETDSSSFALVRNNLAVSYLERTLGQRSENVERAIEL
                     YEEALQVWTRTAMPLEWAKSMTNLGSAYAVRVKGDRQGNQGLAVSCMEQAAEVLNESR
                     VSPFDWAKLQTNLATALSFGAGDLPLRDQARAINLFQAALTVFKPDQYPLKCREASFS
                     LGSLYFQDENWSEAYKYFEIGLEAA"
BASE COUNT         1179 a         1190 c         1220 g         1104 t
ORIGIN      
        1 tacgaggtcc gagttagcgg cggttacgga gatatttcgc aatactccat cacgcagtca
       61 ccgggccagg cagaccatgc cctccaatac ctctttgaaa cagacagtag tgccagatcc
      121 tactcgccct acaagtcctt gaaggacttc tcaaatcaag ggaaaatatt ttacgagcct
      181 cttcgaaaga gaatgaaacc cactgcacca cgttacgtca acatcaatat tgtgggaggc
      241 aagaaacaca cgctcattcc tcagacccaa agtctagaag caaacagaat gtactacgtt
      301 cggataaaca tcggaacatt gtcgccggat accgctgttg agaatcccaa gccatttccc
      361 gcccaacttc ttccgatcgc aaaaggcggt cactggttgg aaacggttgt cgttagcgac
      421 gactttaagt ttgaaaaaca tcgctataga ttttttcttc ctaacagagg cgctagctgg
      481 gtctgcacct gcccgcccca cgatcctcac acctgcgacg atcgcacgcg cgagaattac
      541 ctttacattc caaccaggcc gataaaagaa attcccgaag cccggctgcg gattggaatt
      601 tattacggaa ataatctcat tcaatctcaa ttactcactg ctgtaattgg ggaaactgaa
      661 cgtgagagcc ggggttatag ttcgaagata gattatacgc tgacggcaaa tgtgaacaat
      721 ttggcctcct tcacgccgcg cactctgaat attctcacga accaaaatgc tgacggaacc
      781 catagaattg tcattaacgg ggagctcgat gatgctgtga ccttcaatct gaccgaaggg
      841 aaagttggca atgccatcgg tgcggtgcgt cacaagctcc ttgagttgca catgaggaga
      901 tatggagggc aactaggcag taaaccgcag tttgaaaatc tatacgacaa caataacgcc
      961 aaatcaaagg ttgattttct tgatgacctg aagcaactcg caccttgcgg taggctattg
     1021 tgggaattgc tattgacaga tcaaccagaa cggcgccgaa aactccgcaa tgagatactg
     1081 agaaacccgt cgacgataca agtctcacgt gttcaaggaa gtaatttcgt tttcccctgg
     1141 ggggtcgttt acgatattcc actggaatct gactctgagc agcaccggcc ttgccgcctt
     1201 ctagacgagt gggagcaagc cggcgttctg atcgatgcgg tgcaacctca ctgcccctat
     1261 gacaacagcc atgggaagaa tactatttgc ccgtttggct tttggggctt caaacacatt
     1321 attgaacagc caccctcgat gccggatggt cgtgatctgc aaattgccat caacgttgcc
     1381 aatcgcccca tcgaactgct tgtcgccctc agcctggatc tggatcaaag cctctccaag
     1441 actcacattg aggcgcttga aaccgacctt gtaaacatta tgaaggtggt tccatgtcgt
     1501 tctcgcagaa acatcgaggc ggcgttggca caaccaaaac tcgagttggt gtatttctac
     1561 tgccatggag aaaggcaagc agtctcaggt tcgaacgttc ccgtcccgtt tctcgaaatc
     1621 ggtcatcagg agaagctcac aactggtgac attattagct ggtcagatag tgactgggac
     1681 aaacatcatt ggttggacac ctcacctttg gttttcatca atggctgcca cacagctgag
     1741 ttaacgccgc agattctcgt caattttgta gatgctttca cgggcgtatt tgccgcgggc
     1801 gttatcggca ctgaaatcat gttacaccaa cgggtagcaa atgaagctgg gcagatcttt
     1861 tttgattact tcaagaatcc caaatgctcg gttggggagg cgttgcaaca ggtacgacgc
     1921 tccctgttac tgaagggcaa tttgctgggc ttggcttaca ccgcatactg ctctgcagac
     1981 ttacagcttt cgacttgaag gtacactagc caaggaacga ctgcgttccg ctgacaagga
     2041 gaactcaaat gtcttttaca ataatcggct cagcttgttt cggcttcgta ataggatgga
     2101 taacccttcg cacactacga cgtaaagaag gagcggctgc actatctgat attgcaaccg
     2161 tgatcggagc tgtcggaggc gcgggggtta cagcattgtt caagagtgaa agcctgtttg
     2221 ctggatactc aatgggactc tttatcggct ttgtcgctta tctgattgtc ggcctcatta
     2281 tcggtggtcg tgatgatttc aagggctgga tgggtacccc ttgaagtatt ccctgtgcgt
     2341 tgtcattttc cgttagctga aagacctcca cgttcaccca gggacttaga acgcttcttt
     2401 aggactctgc ttgcgccggt catctattgc cacatgacga tgagcgaata ctccaaagag
     2461 atcgtgacgc tgattcgggt catcctggat agccccaaga attattcgga tggatggctt
     2521 ttcagaatta caaatactac tcgcagacag ttatgaggca gttgacaata ctgaatcagc
     2581 gaagatccat gcgacagccg ctatcgagtt cgcccaaaga actgggcacg atgatgctgt
     2641 ggctcaggtc acagcgtact tagatcaact ggagtcgcgc gatacacctt caactaagca
     2701 gaaacgagcc ttttactgtt ttgaaaaact gacaggcaga ttggtatgat ctggactcgg
     2761 gctcgggatg gcttcgcaat tggcgttttg aacgacgtgc tgtcaagtcc cgatttcagc
     2821 cacaaccagc cgctcgacgt gaagtgctca gcgtgcggac atgaatattc aggcgagctg
     2881 tggttaatcg tggacgtgct ggcccgacgg gacctctacc gtcgtctggt cgacggcaca
     2941 ttgcacttct tgagatgtcc gctggagttg accgtcgccg tggcggtaga ggtccccttt
     3001 ttgttgtttc gtgccgactc agacttaaag ctgatcttct gcgtgccttc gggaacgcaa
     3061 cctgaggaga ttgagcctca agcgaaaatg tgcctggatt tgttgcaggc gcgcatgggc
     3121 gatgcatggg acgagtcctg gccagagact gccatgggga gagccctgga cattggcctg
     3181 cactccatgg tctcggacga atggagcgat cttaggtctt acggcgaaca cgatccgttc
     3241 ctcgaaaaac tcataagttt tccggaagtg aaagatcttg acgagaagcg gcagctcctc
     3301 ttccacgagc ccgatttgtt gggtcgaagc gctataaaca ccctgggctt ttttagccaa
     3361 tcgctaatgg aacaggggaa accgatcaaa gcccatgatg tcgaatcgca aaggcggctc
     3421 ctggaccgtt gcacagaaat agggatcgac gcggcgttcg ctgagttcag ggaacaatac
     3481 ccgccaccac taagcgtcgg caccgtggat gcagaaacca gggcgatgct ggaagatgcg
     3541 atagctatcc cgaccggtct gggggatgtg ctccggaaaa tcgaattgtg cgagaacctc
     3601 ctcacgcgtc tcgaccccca ccaaaactcc ttcttccggg tgatggtgca aaacacgctc
     3661 gcgagacgct attacgaaag ctcggccgga gatcggcgag ccaacgtcga gcacgctatt
     3721 gagttgttaa cgtcaatatt gtcaggactg aatccgcagc aggatcccga ggcgtgggcc
     3781 agcgccattg tcagcctctc gaatgcatat caattgcgga tcgaaggaac acccgcagcc
     3841 aacttcagaa aagctatcga actctgcgaa caagcactca aacacatttc tgagaaagag
     3901 tatccagact cctactgccc cttgatggat gcgctcgcca gcgcctacgt ggacgatccg
     3961 ggaggcgatc gcgttgacaa tatcgaacgg accattgccg ctcatgaaaa gatcctgcag
     4021 atcgaaaaga aacccgacgc gcggaccata tctttgcata acctggccag ggcatacggc
     4081 ctgcgcattc gcggctcccg tgaagtgaat gtggagcgtt cgattgaatt gtatgaagcg
     4141 gcgttagccg gaatctcggc cgaaactgat tcgtcaagct tcgctttggt gagaaacaat
     4201 ctggcggtca gttacctgga aagaacgctg ggccagcgct ccgaaaacgt agagcgggcg
     4261 attgaactgt atgaggaagc gttacaagtc tggactcgta ccgcgatgcc cttagagtgg
     4321 gccaagtcta tgactaatct aggcagtgct tatgccgtgc gtgtgaaagg agatcggcaa
     4381 gggaatcaag gtcttgccgt tagctgcatg gagcaggctg cggaggtcct taacgagtcc
     4441 agggtttcgc cgtttgattg ggccaagctt caaactaatc tggcgaccgc gctgagtttc
     4501 ggagcaggtg acctgccact gcgagatcag gctcgagcaa tcaatttatt ccaggcggct
     4561 cttaccgtct tcaaaccgga tcaataccct ttgaagtgtc gtgaagcctc tttctccctg
     4621 ggaagtctct attttcagga cgagaattgg tctgaagcat acaagtattt cgagatcggc
     4681 ttagaagcgg ctg
//
LOCUS       DASUTG010000222         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1221229, whole genome shotgun sequence.
ACCESSION   DASUTG010000222 DASUTG010000000
VERSION     DASUTG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221229"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VF089_04950"
     CDS             <1..491
                     /locus_tag="VF089_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6173341.1"
                     /translation="VDILLAFPGILLAIAFTALLGPGLDHVILALCLIGWTGYARLVR
                     GEILSLREREYVQAALALGCSPARVITVHLLPNLLPLLLVQATFGMAAAITAEGSLSF
                     LGLGVEPPTASWGSMLNEGRQFLLVAPHLTTYPGLAIMLTVLSLNLLGDALQNRLQSP
                     TR"
     gene            653..826
                     /locus_tag="VF089_04955"
                     /pseudo
     CDS             653..826
                     /locus_tag="VF089_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            860..1174
                     /locus_tag="VF089_04960"
     CDS             860..1174
                     /locus_tag="VF089_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6173342.1"
                     /translation="MLQRAQARIWVDFLNSRIHPAASDITHDREPDRARQRLQQHLST
                     LNNEMTGKDFIVGDYSLADVSFIPFYARRERYGVTIEENFPNLKRWGEELVARPAVAS
                     TL"
     gene            1217..2272
                     /locus_tag="VF089_04965"
     CDS             1217..2272
                     /locus_tag="VF089_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6173343.1"
                     /translation="METLLVSPHEIRGIVTMAEAVEAVRTGFREWGENPQLNAPRRRI
                     HIPTGVRVSVHQGGVPAAGATGLMTHCEWVKPMAQEQVYPRLNHPVIVLYDAAEGELK
                     GIIIGEITCSELPNNIAVTGLRTAATSAVGTDLLARHDASTLGIFGAAGQAKNHLLAL
                     MQVRRLKNVKVYSRNPENRKRFAEEMGPLTNLNITPVSSPQEAVRDVDIILAATNSSV
                     PVFDGNWLEPGQHVTTIVGSNVGLVKGGFTAAKRREIDDATLARSHVRGIVSVQQAVQ
                     DEQADIFDPVNRGVIKWEQLIEIGAILAGQDDGRTRPDQITFYKNNAGQGVADVALGA
                     LVLEKAKKKQAGQKLEV"
BASE COUNT          603 a          671 c          677 g          540 t
ORIGIN      
        1 ccgtggacat tttgctcgcg tttcccggca ttctccttgc catcgcattt acggcactgc
       61 ttggccccgg cctcgatcac gtaattcttg cgctctgcct aatcggctgg accggttacg
      121 cccggctcgt ccgtggtgaa atcctgagct taagagagcg ggagtatgtc caagcagcgc
      181 tcgccttggg ctgcagcccc gcccgggtaa tcaccgttca tttgctgcca aaccttcttc
      241 cgctgcttct cgtacaagcg actttcggca tggcagcggc aataacagcc gaagggagtc
      301 tgagcttttt aggtttaggg gttgaaccgc cgaccgcctc gtggggttcg atgctgaacg
      361 aaggaagaca atttctactc gtggcgcctc atctgacgac ctacccgggt ttggcaatca
      421 tgcttacggt attaagcctc aacctattgg gcgacgcttt gcagaatcgg cttcaatcgc
      481 caactcggta gagaagtaga atgtgttaaa cagtagtgag cacttacggc tcgcttcttc
      541 ggggatttac ttggattaag cgacttgaac catttgcacc cagttgatat tgacctcaac
      601 aaggagggca cctgtgaaac tgtattcctc ttatacgtga ccttatgcac ataggacaag
      661 gattgtcctt tccgaaaaga agatctccta tgagctcgtg gaagtcgatt taaaaaacaa
      721 gcctaaggat ctgctcgatc tgaatcctta cgccaaagtt ccggtgcttg ttgatggtcc
      781 cggagtgatt tatgaatccg cgatcatcaa tgagtatctc gatgagaaat ttcccgaact
      841 gccgctgctg ccgaacaatg tgctgcaaag agctcaagcg aggatttggg tcgatttcct
      901 aaacagtcgg atacacccgg ccgcaagcga tatcacccac gatagagagc ccgatcgcgc
      961 gcgccaacgt ctgcagcagc atctcagtac gctaaacaac gaaatgaccg gcaaggattt
     1021 catcgtcggc gactactcac tggcggatgt ttctttcatt cccttttacg cacggcggga
     1081 acgatacggc gtcacgatcg aggaaaactt tccgaacctg aagcgctggg gagaagaact
     1141 cgtcgcccgt ccggccgtag cgtcgacatt gtagaacgct ttgattattg aaccgcagtc
     1201 gcaacaggag aatagaatgg aaaccttgct cgtcagtccc cacgaaatta gaggcatcgt
     1261 gacgatggcc gaagctgtcg aagcggtccg cacaggtttt cgtgaatggg gcgaaaatcc
     1321 tcagctaaat gcgccgcgcc ggcggattca cattcccacg ggtgtgcgcg taagcgttca
     1381 tcagggtggc gttccggcag ctggagccac cgggctgatg acccattgcg aatgggtcaa
     1441 gcccatggcc caagaacagg tctatccgag actaaaccac ccggtgatcg tgctctacga
     1501 tgccgccgaa ggcgagctca aaggaatcat tatcggtgag atcacctgtt cggaactgcc
     1561 caataacatt gccgtgaccg gcctgcgcac ggcggccacc agcgcggttg ggaccgattt
     1621 gttggcgcgc cacgacgctt ccaccctcgg aattttcggc gccgccggtc aggcaaaaaa
     1681 ccacctgcta gcgctcatgc aggtgcgcag gctcaagaac gtgaaggtct atagtcgtaa
     1741 tcccgagaac cgcaaacgct tcgccgaaga gatgggacca ttgacaaatc tcaatattac
     1801 gcctgtatcg tcgccacagg aggcggttcg cgacgtcgac atcatattag cagcgaccaa
     1861 ctcgagcgtt ccggtctttg acggcaattg gctcgagcca ggccaacatg tcacgaccat
     1921 tgtcggcagc aacgtcggtt tggtgaaggg cggctttacc gccgccaaac gccgtgagat
     1981 cgacgacgct acccttgcgc gcagtcacgt gcgtgggatc gtttcagtgc aacaagcggt
     2041 tcaggacgag caggctgata tcttcgatcc cgtcaaccgc ggcgtcatca aatgggaaca
     2101 gttgatcgag ataggcgcca tcctcgccgg ccaagatgac ggccgcaccc gccccgatca
     2161 gatcactttc tacaagaaca atgccggcca aggcgtagcc gatgtagctt taggagcgct
     2221 ggttttagag aaagcaaaga aaaaacaggc agggcagaag cttgaggtgt agaggcggag
     2281 aaatgagtag ctgaggtatt tcgaagaagt gatgaccgct agatcctgat taactcagaa
     2341 caaaatggct gcaacgagat ttatatgagc agagtagcga tcgtccagat tcaatcttca
     2401 accgagaagc agaaaaatat ccggcgagct ctcgagctgg tcaaagatag taagagcgcc
     2461 ggcgccgatc tggtagtctt tcccgaattt t
//
LOCUS       DASUTG010000223         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1518190, whole genome shotgun sequence.
ACCESSION   DASUTG010000223 DASUTG010000000
VERSION     DASUTG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518190"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..464)
                     /locus_tag="VF089_04970"
     CDS             complement(81..464)
                     /locus_tag="VF089_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173344.1"
                     /translation="MTARPQILFLLAMILIFAGCNKSYDEKIGGMEIPIPGGLKKSDG
                     AGIEVALLGFGGGQSSFRGQLEPGKVIEFYEKEMPLRGWQPNAGVISKGGMLTYAKDN
                     RTVIIMVGGNNDATTMTVTVGRTGR"
     gene            complement(575..1753)
                     /locus_tag="VF089_04975"
     CDS             complement(575..1753)
                     /locus_tag="VF089_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173345.1"
                     /translation="MTDNHHSFTRAEIGPVIVVSLISALRLLGIFLVLPVFSVYAVHY
                     PEASLAMAGVAFGSYALTQSILQIPLGWASDRWGRKPILLIGLALFGVGSIGCGLAQS
                     ISQLIVARVIQGTGAIGSVALAALADLTRPAVRTQAFTVTGIAIGSAFMIGIMGGPLL
                     AASIGLSGLFYVLAALAFSAMILAAISFPKKNQFIDKQSALPFKPMLMSGELRPIFIA
                     AFVLSFTLNLFFFTYPLSWAEIGLDRAELWKVYLIIFLPSALLVFPYMRRAERLGRFR
                     MPIMIGWLTAATGYAVYLVGAQSDLLLYASGAAFFFGYSLFQPLLPTFLTKRIPQAGR
                     GTATGVYSFFGFIGSSLGGMLGGVLIHISPSLPEFLGVVLLVLWYFSGLPTAPEPASR
                     "
     gene            complement(1869..2786)
                     /locus_tag="VF089_04980"
     CDS             complement(1869..2786)
                     /locus_tag="VF089_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX6173346.1"
                     /translation="MFGISFEAYLPKLVDFGINFAQSSFRVLLIIVAALLAIKFLRLG
                     LNRLEVILVRAREASESVPGAAAMRIRTLMSVLWTIIAGLVWFTAVLTILGQVGVNVT
                     PILASAGVVGLAVGFGAQNLVKDFVSGFFLILENQIRVGDVAIINGTGGLVEAITFRT
                     IVLRDQAAVVHVFPNGSINTLSNMTKDWSAYVMDIGVGYKEDTDHVIEVMRRVGGELQ
                     ADPQYGSAMLEPIEIIGLDAFTDSAVIIKARLKTLPIRQWMVGREYRRRLKKAFEAEN
                     IEFPFPQRTVHVGEAKAPLTLAVKQSEEN"
BASE COUNT          712 a          781 c          756 g          560 t
ORIGIN      
        1 tcattctcta gttgctgctt caccttcgaa cctaccgata acgtgatttt tcctggtcat
       61 ttggatggtt ccaaaagaaa tcaacgaccc gttctgccga cagtgactgt catagtggta
      121 gcgtcgttgt tgccgccaac cataatgatg acggttctat tatctttggc gtaagtcagc
      181 atgccgccct tagatatgac gccggcgttg ggctgccaac cacgtaacgg catctccttt
      241 tcataaaact caatcacctt cccgggttcc agctgaccgc gaaatgagct ttgaccgccg
      301 ccaaaaccga gcagggcgac ttcaatcccc gctccatcgg atttcttcaa cccgcccggg
      361 atcgggatct ccatgccgcc gattttttcg tcataggatt tattgcagcc ggcaaaaatt
      421 aaaatcattg ccagcaggaa aagtatttgg gggcgggcgg tcatagcggc agttcggcga
      481 tttgactagc tttcgcgctc ttcaccatct acaccaatga cacggtcgcg gcaacgcgaa
      541 tcgatggagg atctaaatcg tgagagcaac ggctttatcg agaggcaggc tccggcgcgg
      601 tgggtagccc ggaaaaatac caaagtacca aaagcacgac accgagaaat tctggcagcg
      661 acggagagat atggatcagc acgccgccga gcatgccgcc gagtgacgag ccgatgaagc
      721 caaaaaaact ataaacaccg gtggctgtgc cgcggcccgc ctgagggatt cgcttggtca
      781 gaaatgtcgg caacagtggc tggaacaagc tgtaaccgaa aaagaaagcc gcgccgctgg
      841 catataacag caaatccgac tgagcgccga ccagatacac agcatagcct gtggcggccg
      901 tcagccagcc gatcatgatt ggcatgcgaa aacggcccaa tctttcggcg cggcgcatgt
      961 aagggaatac caacaacgcg ctgggtaaga agataatcag atacactttc caaagctcgg
     1021 cgcgatcgag tcctatctcc gcccagctca gcggataagt gaaaaagaaa aggttcaggg
     1081 tgaaagaaag aacaaaggcg gcgataaaaa tcggccgcag ctcgccgctc atcaacatcg
     1141 gtttaaaagg cagcgctgac tgtttgtcaa tgaattgatt ctttttagga aaggagatcg
     1201 cggctagtat cattgccgaa aaagcgagcg cggccaagac atagaacaag ccgctgagac
     1261 caatgcttgc cgccaaaagc ggtccgccca ttatgccgat cataaaggct gagccaatgg
     1321 caattccggt aacggtaaat gcctgggtgc gaaccgccgg ccttgtaaga tcagccagcg
     1381 ctgccagagc tacggatccg atcgcgccgg tgccttgaat cactcgtgcg acgatgagct
     1441 gggaaatact ctgcgccaag ccacaaccga tgctgcccac tccgaacagc gccaggccga
     1501 tgagcaatat cggcttgcgt ccccagcgat cgctggccca tcccagcggg atttgcagga
     1561 tactctgagt cagggcatag gagccgaaag caacgccggc catggcaagg ctggcttcgg
     1621 gataatgaac agcgtagacg ctgaacactg ggaggaccag aaagatgccg agcaaacgca
     1681 atgcggatat cagactgacg acaatcaccg ggccgatttc agcgcgggta aaagaatgat
     1741 gattgtcagt caatgtgttg cgcgagctcc aagatcccgg gtccgggaaa aataatcagt
     1801 ccaatttaca cgcttgggcg aaaatgggaa ggcttgcgag catcatcgca ggttgattca
     1861 cctggctgct aattttcttc agactgtttg acggcaagtg tcaacggcgc tttggcctct
     1921 cccacatgaa ccgttctctg tgggaacgga aactcgatgt tttcggcttc gaaggctttt
     1981 ttcagccggc gacggtactc tcgacccacc atccactgcc ttatcgggag tgtctttagc
     2041 cgggccttga tgatcacggc cgaatccgtg aacgcatcca gaccgatgat ttcaatcggc
     2101 tcgagcatcg ccgaaccgta ttgcggatcg gcctgcaact cgccgccgac tcgacgcatc
     2161 acttcgatga cgtgatcggt atcttccttg tatcccaccc cgatatccat gacgtaggcc
     2221 gaccagtcct tggtcatatt ggacagcgtg ttgatcgagc cgttgggaaa aacgtgaacc
     2281 acagcggcct ggtcgcgcag gacgattgtg cggaaggtga tcgcttcgac cagaccgccg
     2341 gttccgttga tgatcgcaac atcgccgacg cggatctgat tctccaaaat cagaaagaaa
     2401 ccgctgacaa agtctttcac caggttttgc gcaccgaagc ccactgcaag tccgaccacg
     2461 ccagcgctgg cgagaatcgg cgtgacgttt accccgactt gtccgaggat tgtcaaaacc
     2521 gcggtgaacc agacgaggcc ggcgatgata gtccagagca cgctcatcaa ggttcggatg
     2581 cgcatcgccg ccgctcccgg caccgattcc gaagcctccc gtgcccgcac aaggatcact
     2641 tcgagccggt taagccccag gcggagaaac ttgatcgcga gcaatgccgc tacgatgatt
     2701 aagagaaccc gaaatgacga ctgcgcgaaa ttgatgccga aatcgacaag cttcggtaga
     2761 taggcttcga acgagattcc aaacattctc tatcctcctg gaggcaggc
//
LOCUS       DASUTG010000224         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1666541, whole genome shotgun sequence.
ACCESSION   DASUTG010000224 DASUTG010000000
VERSION     DASUTG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666541"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF089_04985"
     CDS             complement(<1..516)
                     /locus_tag="VF089_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6173347.1"
                     /translation="MQTDRYTYSPIIRRAPIHWPNKARVALMIAPNIEFFHIDKVIPG
                     ASSSQLPDVTGYALRDYGSRIGVFRMMEVLDKHGIRATVLLNSDVCAHHPAIIEEGNK
                     RGWEWLGHGVTNNIRMNQYPAEEERAVIRQIKETIASATGKAPRGWLGPGGGDESPHT
                     LDHLAAEGFDYV"
     gene            720..2249
                     /gene="gndA"
                     /locus_tag="VF089_04990"
     CDS             720..2249
                     /gene="gndA"
                     /locus_tag="VF089_04990"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX6173348.1"
                     /translation="MATEELADIGLIGLAVMGENLALNIADHGFRVAVYNRTTQVMKD
                     FIAANPDTPGGLIGCASLPELLAAIKKPRKIIILVKAGPAVDTVTQQLVDAGLEKDDL
                     VIDCGNSLWTDTIRRERDYAGHCRFFGSGVSGGEIGARFGPSLMPGGDAQAWQHLEPI
                     WKAIAAKVDPETGKAIETAKPGKPVEGGIPCTAYIGENGAGHYVKMIHNGIEYIDMQL
                     ISEAYFLMRKLLGAEPDQLSQIFAGWNEGDLDSYLIEITADILNQRDPQKPDSFLVDD
                     ILDTAQQKGTGKWTSVNALDMGIPANAIAEAVFARYLSALKDERVGASKQLHGPEYTY
                     SGNRQELIDSVRDALYCSKICAYAQGFQLMRAAQGEYNWKLDFASIAAIWRGGCIIRA
                     RLLQKITEAYRREPQLVNLLLDPYFNQQIQRTQSRWRTVVALAAQAGIAVPAFMSALA
                     YYDGYRSAQLPANLLQAQRDYFGAHTYERTDQPRGRFFHLDWPAPDRPELAIADSGKF
                     S"
BASE COUNT          524 a          674 c          652 g          515 t
ORIGIN      
        1 aacgtaatcg aacccttcgg ccgccagatg atccagagta tgcggactct cgtcgccacc
       61 gcccgggccc aaccaacccc gcggcgcttt gccggttgcg ctggcaatgg tttctttgat
      121 ctgccgaatc accgcgcgct cctcttcggc cgggtactgg ttcatccgta tgttgttggt
      181 aaccccgtgg ccaagccact cccagccgcg tttattacct tcttcgataa tcgccggatg
      241 atgtgcgcaa acatcagagt tcagaagcac cgtcgcgcgg atgccgtgct tgtccaggac
      301 ctccatcatg cgaaacacac cgatgcgcga accgtaatcc cgcaacgcat agccggtgac
      361 atcgggcaat tgagagctgg aagcgccggg aatgactttg tcgatgtgga agaactcgat
      421 gttcggcgcg atcatcagcg cgactctggc tttgttgggc cagtgaatcg gcgcgcggcg
      481 gataatcggc gagtaggtat aacgatcggt ttgcatttac aactccttga gcttgactgg
      541 aatgatacct atgacacgcg agatatttag gtcaagattt ttttgccacg gtgctctgcg
      601 ccggcgctcc aatcaaccgg aggtaaccct tgtggcgtgc ttttgttttt tctgccggct
      661 gctagactcc tcacaagcag cacacaccgc ccgagacttt agcgaaaagg agataaccca
      721 tggctactga ggaactggcg gatatcggcc taatcggcct ggccgtcatg ggcgaaaacc
      781 tggccctgaa cattgcggat catggcttta gagtagccgt ttacaaccgc acgactcagg
      841 tcatgaaaga tttcattgcg gccaatcctg acacgccggg cggtctgatt ggatgcgctt
      901 cgttgcctga gctactggcg gcaatcaaga agccgcgcaa aatcatcatc ctcgtcaaag
      961 cgggcccagc cgtcgatacc gtgacgcaac agctcgtgga tgccgggctg gaaaaagacg
     1021 acctggtaat cgattgcggc aacagcctgt ggaccgacac catccggcgc gagagagact
     1081 atgccggtca ttgtcgcttc ttcggctccg gcgtttccgg cggtgaaata ggcgcgcgct
     1141 tcggcccgtc gttgatgcca ggcggcgatg cgcaggcgtg gcagcatctc gagccgatct
     1201 ggaaagccat cgccgccaag gtcgatcccg aaaccggcaa agcgattgaa accgccaagc
     1261 cgggcaaacc tgttgaaggc ggaattcctt gcacggccta catcggcgag aacggcgccg
     1321 ggcactatgt caaaatgatc cacaacggca tcgagtacat cgacatgcag ttgatctccg
     1381 aggcttactt tttgatgcgc aaattgcttg gcgccgaacc cgatcagctc tcgcaaattt
     1441 ttgccggttg gaacgaaggc gatctcgatt cttatctgat tgaaattacc gctgacattc
     1501 ttaatcagcg cgatccgcag aaaccggatt catttctcgt cgacgatatt ctcgacaccg
     1561 cccaacagaa aggaaccggt aaatggacca gcgtcaacgc actggatatg gggatcccgg
     1621 ccaatgccat cgccgaggcg gtctttgcac gctatttgag cgcgcttaaa gatgagcgag
     1681 tcggggcatc caaacaacta catggcccgg aatacaccta tagcggaaat cgtcaggaac
     1741 tgatcgattc ggttcgcgat gcgctctact gttcgaagat ctgcgcctac gcccaaggtt
     1801 ttcagctcat gcgcgccgct cagggtgaat acaattggaa gctcgatttc gcctccatcg
     1861 ccgctatctg gcgcggcggc tgcatcatcc gcgcccgttt actgcaaaaa atcaccgagg
     1921 cctataggcg cgagccgcag ttggtaaacc tgctgctcga tccctatttc aaccagcaga
     1981 ttcaaagaac tcagtcgcgc tggcgcaccg tggttgcttt agctgctcag gcagggatcg
     2041 ccgttcccgc attcatgtcg gcattggctt attacgacgg ctaccggtcg gcgcagctgc
     2101 cggcaaattt actccaggcg cagcgagatt atttcggcgc gcatacgtac gaacggacgg
     2161 atcaaccgcg cggccgattt tttcatctgg actggccggc gccggatcgc ccggaactcg
     2221 caatagcgga ttcaggtaaa ttcagctgaa gcatcagctg tgagattctc agtaaagcgc
     2281 tgtcggtata cgtagaagaa gcgcggggta gctttttacc ggcagatttg aaagaacttg
     2341 actgttacat gcccgttgct gttac
//
LOCUS       DASUTG010000225         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_332606, whole genome shotgun sequence.
ACCESSION   DASUTG010000225 DASUTG010000000
VERSION     DASUTG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332606"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1041
                     /locus_tag="VF089_04995"
     CDS             100..1041
                     /locus_tag="VF089_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173349.1"
                     /translation="MEYRQLGNAGVRVSVIGLGANRFGSKEVGQADVDNIINRALELG
                     INFLDSADVYNEGRSEETLGEALKARMDQVVLASKFSFPRKTAPNSWGASRYHMMQAL
                     DASLRRLQTDHIDLYYIHRWDASTPIEETLRALDDVIRMGKVRYIGASACASWQLAHA
                     NVLAELKGWSSFVVIQSEYNLLRRDVEREVLPYCRAHQVGFVPYYPLAGGFLTGKYEQ
                     GKPAPPGSRGATARNVQEFMTEANYNRIGRLANWAKARGRGVNELAQAWLLAQPPVCS
                     VISGATKTEQVISNVRASDWQLTPEELKEVDEIIEFR"
     gene            complement(1046..1615)
                     /locus_tag="VF089_05000"
     CDS             complement(1046..1615)
                     /locus_tag="VF089_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173350.1"
                     /translation="MKKRLTRRAQEMIARALPHFPELNGKTITVGYTRKHLGSASIIY
                     RGGKIARLIIRLKVRTLTYQTIGHELTHLVQALVRGDRPQIESIDSPKIPYGEKPCDI
                     WTLARHELFCDDPPTYITMPRIMRERWFDYAEPVRRLCIAAIQQRITRRLYIQWLEKE
                     VGKLAEAAPRRQTFPAQLTLPFADSKNAD"
     gene            1718..>2374
                     /gene="era"
                     /locus_tag="VF089_05005"
     CDS             1718..>2374
                     /gene="era"
                     /locus_tag="VF089_05005"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6173351.1"
                     /translation="MTKESPKNKRDKEPLFRSGYVAIVGRPNVGKSTLLNNLLGEKVA
                     IVSPKPQTTRNRITGIKTSSRSQIVFLDTPGIHQARSLMNRRMVDVALQSLHEVNGVL
                     WLLDSHSRIGPEDERIAATLRGVKSPVFILLNKIDLVSKGKLLPLIQRCAELLPGKEI
                     IPISALKGENLPLVLDLVEKALPEGPKFFPEGEFTDQSERFLAAEIIREKVFLLTHEE
                     I"
BASE COUNT          534 a          636 c          685 g          519 t
ORIGIN      
        1 gagtcgatct catggaccta ctcgccggaa aaatgtcgtc tctctctctg agttcctgcg
       61 aaacagataa gtctttctca ctcacctgaa ggagtttcca tggagtaccg ccagctgggc
      121 aacgccgggg tgcgcgtttc cgtgattgga cttggcgcca atcgtttcgg ttcgaaggaa
      181 gtgggccaag ccgatgtcga caacattatc aatcgagctc tcgagcttgg gattaatttc
      241 ctcgacagcg ccgacgttta caatgaaggg cggtcggaag aaaccttggg cgaagcctta
      301 aaagcgcgca tggatcaggt cgtcttggcc agcaaatttt catttccgag aaaaaccgct
      361 ccgaactctt ggggcgcatc gcgttatcat atgatgcagg cccttgacgc gagtctgcgc
      421 cggctgcaga ccgaccacat cgatctttat tatattcatc gctgggatgc atcgacgccg
      481 atagaggaaa ctttgcgcgc gttggacgac gtcatccgta tgggcaaggt gcgctacata
      541 ggcgcctcgg cttgcgcctc atggcagttg gctcacgcca atgttttggc cgaattgaag
      601 ggctggtcct cctttgtggt gattcaatcc gagtacaatc tcctgcggcg cgatgttgag
      661 agagaagtgt tgccttactg ccgcgctcat caggtgggct tcgttcccta ttatccgctg
      721 gcaggcggct ttctcaccgg caaatacgag caaggaaaac cagcgccgcc gggctcacgt
      781 ggcgcgactg cgagaaatgt gcaagagttt atgaccgagg cgaactacaa tcgcataggt
      841 cgattggcaa actgggccaa ggcgcgaggg cgcggcgtca atgagctggc gcaggcatgg
      901 ctactggcgc agccgccagt ttgctcggtg atctccggcg cgaccaagac tgaacaagtc
      961 attagcaacg taagagccag cgattggcag ctaacacctg aagagctcaa agaagtagac
     1021 gagattatag agttccgatg actattcagt cggcgttctt cgaatcggca aagggcaagg
     1081 taagctgcgc gggaaatgtt tgccgccggg gcgcagcctc ggccagcttg ccgacctcct
     1141 tttcaagcca ttgaatgtaa agccgacggg tgatccgctg ttggatcgcg gcgatgcaca
     1201 gcctgcgcac cggttcagca tagtcgaacc aacgctcgcg catgatgcgc ggcatcgtga
     1261 tgtaggtggg tggatcgtcg cagaagagct cgtgccgtgc cagcgtccag atgtcgcacg
     1321 gcttctcgcc gtacggaatt tttggtgaat cgattgactc gatctgcggg cgatccccgc
     1381 gcaccagcgc ctgcaccaaa tgcgtgagct cgtgcccgat cgtctggtaa gttaatgtcc
     1441 gaaccttaag ccggatgatc agtcgcgcga ttttaccgcc gcgatagatg atgctcgcgc
     1501 tgcccaggtg cttgcgggta tatcccacgg tgatcgtttt tccgttcaat tccggaaagt
     1561 gcggcagagc gcgcgcgatc atttcctgag cgcggcgggt aagccgcttc ttcatcaaag
     1621 gtgtaagcct aagcggcacg gattcgagct gctttgaggt cgacgagttt gaacgttgat
     1681 tggaccatgc cataatttag cttatagatc ggaagctatg acaaaggaaa gcccaaagaa
     1741 caagcgcgac aaagaacccc tctttcgatc gggctacgtt gcgatcgtcg gccggcccaa
     1801 tgtcggcaaa tcgaccctgc taaataatct cctcggggaa aaagttgcga tcgtctcgcc
     1861 caaaccccag acgacacgca accgtatcac cggtatcaaa accagctcac gctcgcagat
     1921 cgtgtttctg gataccccgg ggattcatca ggcgcgctcg ctgatgaacc ggcgcatggt
     1981 cgatgttgcg ctgcagagcc tgcatgaagt caatggcgtg ctgtggctgc tcgattcgca
     2041 cagcagaatc ggcccggagg acgaacgaat tgccgcaact ctgcgaggcg taaagagtcc
     2101 ggtgttcatc cttttgaaca aaatcgactt ggtttccaag gggaagctgc tgccgctgat
     2161 ccaacgttgc gcggaactgt tgcccggtaa ggaaatcatc ccaatcagcg ccctcaaagg
     2221 agaaaacctg ccgctggtat tggatctggt cgaaaaagct ttgcccgagg gaccgaagtt
     2281 ttttccggaa ggagagttta ccgatcagag cgagcgattt cttgccgccg agattatccg
     2341 tgagaaagta ttcttgctca cccacgagga gatc
//
LOCUS       DASUTG010000226         7232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1073739, whole genome shotgun sequence.
ACCESSION   DASUTG010000226 DASUTG010000000
VERSION     DASUTG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073739"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="purH"
                     /locus_tag="VF089_05010"
     CDS             <1..816
                     /gene="purH"
                     /locus_tag="VF089_05010"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX6173352.1"
                     /translation="FNNILDADAALSTVLEFSEIASVAIKHNNPCGVALSKTSLADSF
                     RKAKACDPISIFGGVIAFNRPVDEETAGELKDIFLEIVIAPSFTPAAKALLSSAKRLL
                     NIRLLELDMREPQTGGYDLRRVRGGMLMQDWDTGSVDVRACKVVTDRKPTEREYQALD
                     FAWRVCRHVKSNTIVFAAPDQVLGVGAGQMSRIDSTKIAVLRAATHGLDLAGSAVASD
                     AFYPFRDGLDEAANAGAKSVIQPGGSIKDDEVIAAANEHGMAMIFTGMRHFRH"
     gene            901..2172
                     /gene="purD"
                     /locus_tag="VF089_05015"
     CDS             901..2172
                     /gene="purD"
                     /locus_tag="VF089_05015"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX6173353.1"
                     /translation="MKILVIGSGGREHALIWKLSQSPEVDKIYCAPGSAGIAEFAEVV
                     AIAPHQIDKLADFAAEQKIDLTVVGPELPLTLGISDLFESRGLKIFGPNRDAAQLEGS
                     KAFAKKIMTENSIPTAAAGIFSDAQSARRYLDQNPAPYVVKADGLAAGKGVLICASRE
                     EAHSAIDEIMVEKAFGHAGDKVVIEECLQGEEASFMVLTDGDHVLALPSSQDHKRVFD
                     NDQGPNTGGMGAYSPAPVVTQTVEDRVLREVVTPLMAGLKRQGIVYRGVIYVGLMLTN
                     TGPKVLEFNARFGDPECQVIMMRLKSDLVPLLEATVHGTLRTVRPEWYRDPAVCVVLS
                     AGGYPGSYETGKEIRGLEKLRDWREGFVFHAGTSKENGRWQTSGGRVLGVTARGRDLA
                     EAVQEVYRGVNAISWDRMHYRRDIARRALKG"
     gene            2241..2849
                     /locus_tag="VF089_05020"
     CDS             2241..2849
                     /locus_tag="VF089_05020"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6173354.1"
                     /translation="MNLSQAIASLERGDVVVFPTETVYGLGADALNPAAVEKVFKLKG
                     RNPDTPIPIIVADQAMLKDLVEEIPSIARKLMERFWPGPLTLVLPAVPGTPKQLLNRT
                     GGIGVRISSQPIATQLARDLRRPLTATSANPSGQPAASTIEQAQNYFAGEIEIFLDGG
                     KLPSKSGSSVVEVSDERIKIIREGEISIESLAASIGRKNILA"
     gene            complement(2875..3423)
                     /locus_tag="VF089_05025"
     CDS             complement(2875..3423)
                     /locus_tag="VF089_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173355.1"
                     /translation="MAKQNHMINLSEVPVVEINAPEGSAFGGSRQRVGAAIGAQKLGY
                     SFFSVPPGKAAFPYHTHTTNEEMIYVVEGRGVLRLAKDEVEVTAGTIVACPPGTEFPH
                     QLINTGNQDLRYLVVSTMEYPDLSEYPDSNKVGAYATAAVGKQVGFRALFVKDQQVSY
                     YEGEDGEEIGRIVKSGSRQAAE"
     gene            3483..4478
                     /locus_tag="VF089_05030"
     CDS             3483..4478
                     /locus_tag="VF089_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6173356.1"
                     /translation="MLSEQIMLVLSEKQVEALIEIDELIAALERAHIQYSTGGAVMPV
                     RLVVPLPQIDGRITSMPGFLNEDQALGMKVVTYFHNNPKQNLPSILATIMLFSAESGK
                     MIAVMDGSYITAIRTACASAMASKVLSNPTASELGILGAGVQAKAHLTALARVRKIRR
                     IKIYSPSGTSAGRVKQEVEPHLGVAIEVANSAEEAVRGADLIVTATTAKQPILAAQWL
                     KPGAHINAVGSHRPDLRELDGTTVARSRVFVDSREAIMAECGDVLLALAEKSISADHV
                     QAEIGEVLAGTKPGRTGAEEVTLYKSVGIAIQDVATAHLVYHKALQRGVGTDVAV"
     gene            4521..7175
                     /locus_tag="VF089_05035"
     CDS             4521..7175
                     /locus_tag="VF089_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX6173357.1"
                     /translation="MADLAQVIRQIPLFSALSREDIAKVLGKMEERYYVSGRIIFSQG
                     DQGDAFYLIQTGAVQVVLENRGRAEIIRVLGPQDWFGEMALLSGEPRSATIVALRDSL
                     IWRLSRHAWDELIDKHPTWLLHFCAALSKKLSYVEQQYSQGREAFYSLAEEFYSSRPA
                     PEQALLRRLSLLTVAELQAAEEVLPTRLASQCLATLRENHFPLVRVIDSTRLDLHPFF
                     LDFLREKFAEHEGPEDAKQVHCELAERLEKLGRWQEAIHHCLAAENWSRAAALLSRHS
                     QVLLDGSAAAVREAIEQLPRDQIDLDLMHMKAEALVRLGEIEAAVAYCTEALLDSAKT
                     PRDKATATRLHHAISSLARQGDHAQALNLLRNALELRQQDAVSDLASIDEKFDTAGIA
                     APLRQTHTEFTETNTLGASKYFPGALDKSNLSRWTGALLGIVAWSYLWLADPDIGLEP
                     SATRQLALLVLTLIFWMFRVFPEYGVALLFAIGMIISGLARPEVVLSGFSSSSWFMTL
                     GVLGLGAAITSSGLFYRLSLQLVRVFPLGYRWQIVAMGFMGIVVMALIPQQSARTAII
                     SQMLMNLSESLSYKNPSKASTGLFVASFLGLGQLGFLFLTGSTTSLIAWGLLPEEVRA
                     QFSWGYWFYAASVPTFTVVAIVISATLILYKPESQARVSYKMVQTQLQILGPLSRHEW
                     ITLGVLCLTLGGWLSRSYHNIDGAWVALIALCVLVNTGVLGWGMMKKGIDWEMLLYMG
                     ATLSIPALLTQAKIDQWLVGMFAPVILPFTNWPALCFTVIIAIAIAVKLAFTSFLTVV
                     TLTVALLPLASELGISPWVMAMIVLMGSEVWFLRFQVDWHTMASATTGGKGFSYPMMY
                     RINPVYAVAYILAAIVAIPWWRHLGLIG"
BASE COUNT         1559 a         2007 c         2071 g         1595 t
ORIGIN      
        1 ttcaacaata ttttagacgc cgatgccgcg ttaagtacgg tcctggaatt ctctgagatc
       61 gcaagcgtcg ccatcaagca caacaatccc tgcggcgtgg ccttatcaaa aacctcgttg
      121 gcggattctt ttcgcaaagc caaagcctgc gatccgatat ctattttcgg cggcgtgatc
      181 gcgtttaacc gcccggttga cgaagagact gcgggtgaac tcaaggacat ctttctggaa
      241 atcgtcattg ctccgagttt tacgccggcg gccaaagcgc ttctctcatc ggcaaagcgc
      301 ttgctcaaca ttcgcctttt ggaactcgat atgagggaac cacaaaccgg cggctacgat
      361 ctgcgccggg taaggggcgg catgttgatg caggattggg ataccggatc tgtggatgta
      421 cgtgcctgta aagtggtgac cgacagaaaa ccgactgaac gagaatatca ggcgctcgat
      481 ttcgcttgga gagtttgccg ccacgtgaag tccaacacca ttgtttttgc tgcgccggat
      541 caagtccttg gcgtcggcgc gggccagatg agccggatcg attcgacaaa aattgcggtg
      601 ctccgcgcag caacgcacgg gctggatctt gccgggtcgg cagtggcttc ggatgcgttc
      661 tacccgtttc gcgacgggct cgacgaagcg gcgaatgccg gcgccaaatc ggtgatccag
      721 ccgggcggtt ccatcaaaga cgatgaagtt atcgccgccg ccaatgaaca cggcatggcg
      781 atgatcttta ccgggatgag acacttcaga cattagcaag gacatgcctc gtgccagaag
      841 cctgaagtaa gatgtgatct tcgcattact tctggtcgct ggcttccgaa tcctcagatt
      901 atgaaaatat tggtgattgg cagcggcggg cgcgaacacg cgctgatctg gaaactcagt
      961 cagagtccag aagtcgataa gatctattgc gcgcccggca gcgcggggat cgctgagttt
     1021 gcggaagtag tcgccatcgc gccgcaccag atcgacaagc tcgccgactt cgccgcagag
     1081 cagaagattg atctcaccgt tgttggaccg gagctcccgt tgactcttgg catctcagat
     1141 ttgtttgaat cccgtggatt aaaaatcttc ggcccaaatc gagacgcggc gcagctggaa
     1201 ggcagcaaag ccttcgccaa gaaaatcatg accgagaaca gcattccgac cgccgccgcc
     1261 ggaatcttca gcgatgccca atccgccagg cgctatctcg atcagaatcc ggcgccttac
     1321 gtcgtcaaag ccgacggttt ggcggcaggc aaaggcgtgc tgatctgcgc cagccgggaa
     1381 gaagcgcatt cggccatcga tgaaatcatg gttgaaaaag ccttcggcca tgccggcgac
     1441 aaggtcgtca tcgaagaatg tttgcaaggc gaagaagcct cgttcatggt gcttaccgat
     1501 ggcgatcacg tgctcgcttt gccatcctca caggatcaca agcgcgtctt cgataacgat
     1561 caggggccga ataccggcgg catgggcgcc tactcgccgg cgccggtcgt gactcaaacc
     1621 gttgaagatc gcgtgctccg cgaagttgta accccgttga tggcaggact caaacgccaa
     1681 ggcatagttt accgcggggt catctatgtt ggcttgatgc tgacaaatac tggcccgaaa
     1741 gttctggagt tcaatgctcg gttcggcgat cccgaatgtc aggtgattat gatgcgcttg
     1801 aagagcgatc tcgtgccgct gctggaagcc acggtacacg gcacgttgcg aactgtccgc
     1861 ccggaatggt atcgggatcc ggctgtgtgt gtagtcctaa gcgctggagg ctatcccggc
     1921 tcttatgaaa caggcaaaga gatccgtggg cttgaaaaac tgagagattg gcgcgaaggc
     1981 tttgtctttc atgccggcac cagcaaggag aacggccgct ggcaaacttc cggcggtcgc
     2041 gtcttgggag taaccgcgcg gggtcgcgat cttgcggaag cggtccaaga agtttaccga
     2101 ggcgtcaatg cgatcagttg ggatcgcatg cattatcgca gggacattgc gcgacgcgcc
     2161 ttgaaaggtt agctactcct gccatcggcc tagtgcacaa ccacacctcc aaacagcgcg
     2221 caaatctgga gcagctcctg atgaacctta gtcaagcgat tgcttcgcta gaacgcggtg
     2281 acgtggtcgt tttcccgaca gaaacggtct atgggttggg cgctgatgcg ctcaatcccg
     2341 cggcggttga aaaagtattt aaactcaagg gccgcaatcc cgatactccc atcccaatta
     2401 tcgtggcgga ccaagccatg ttgaaagatc tggtggaaga aatcccgtcc atagcccgaa
     2461 agctcatgga acggttttgg ccgggacctc tgacgttagt gctgccggcc gttccgggta
     2521 cgcccaagca gctcttaaat cggaccgggg gaattggcgt ccgtatctcc agccagccta
     2581 tagcgaccca attggctcgg gacttgaggc gccctttgac cgccaccagc gccaaccctt
     2641 cggggcagcc ggcggcgtcc accatcgagc aggcgcagaa ttattttgcc ggcgagatcg
     2701 aaatttttct ggatggcggg aagctcccgt cgaagagcgg ctcgagcgtt gttgaagtga
     2761 gcgatgagcg catcaagatc attcgcgaag gcgagatcag catcgagtcg ctggcggcga
     2821 gtatcggcag gaaaaacatt ctcgcgtgaa tcgctcgagc cggagaacgc ggaattattc
     2881 ggcggcttga cggcttccgg actttacaat gcggccaatt tcttcaccgt cttctccttc
     2941 gtagtagctc acctgctggt ctttgacgaa caaggcgcga aagccgacct gcttgcccac
     3001 agcggccgtc gcatacgcac caaccttatt ggaatcggga tattccgaga gatccggata
     3061 ctccatcgtg cttaccacca ggtagcgcaa gtcttggtta ccggtgttaa tcagttgatg
     3121 cggaaattcg gttcctggcg gacaggcaac gatagtcccg gcggtcacct caacctcgtc
     3181 ttttgccagg cgcagaactc cgcggccttc cacgacatag atcatctctt cgttggtcgt
     3241 gtgcgtatgg tatggaaagg ccgctttccc tggcggcacg ctgaaaaagc tgtagcccag
     3301 cttttgcgcg ccgatggcgg cgcccactct ctgtcgcgag ccgccgaatg ctgaaccctc
     3361 cggcgcattg atctcgacca cgggtacctc agagagattg atcatgtgat tctgcttagc
     3421 catcgttcct cccgaccgat tcaaatcctt gatgtcgcac actaccactc ggacgcaact
     3481 ttatgctatc agaacagatc atgttggtct tatctgaaaa gcaagttgag gcgctgatcg
     3541 agattgacga gctgatagcc gcgctggaac gagcgcatat ccaatactcc accggcgggg
     3601 cagtgatgcc ggtgcgcctg gtggtgccat tgccgcaaat cgacgggcgg atcaccagca
     3661 tgccggggtt tttgaatgag gatcaggcgc tcggcatgaa agtagtcacc tattttcaca
     3721 acaatcctaa gcagaacttg ccgtcgatcc tcgccacgat catgctgttc agcgccgaaa
     3781 gcggaaagat gattgccgtc atggatggca gctatatcac cgccatccga actgcttgcg
     3841 cttcggcaat ggcgagcaag gttctgtcca acccgaccgc atcagaacta ggaattctcg
     3901 gggcaggggt ccaggccaaa gcgcatctaa cggctctggc gcgcgtgcga aaaattcgtc
     3961 gcataaagat ctacagcccg tcaggtacga gcgccggccg tgtcaaacaa gaagtcgaac
     4021 cacacctcgg agtcgccatt gaagtggcca acagcgcgga agaagcggta cgcggagccg
     4081 atctcatcgt caccgccacg acggcaaaac agccgatcct agccgcccaa tggctgaagc
     4141 cgggcgcgca catcaacgca gtgggctcgc accgtcctga cctaagagaa ctcgatggca
     4201 caactgtggc gcgctccagg gtattcgtag attcccgcga agcgatcatg gccgaatgcg
     4261 gcgacgtcct gttggcgtta gcggagaagt cgatcagcgc ggatcatgtg caggcggaaa
     4321 tcggtgaagt tctcgccggc acaaaaccgg gaagaaccgg agccgaagaa gtaactctct
     4381 acaaatccgt gggcatcgct attcaagatg tcgcgaccgc ccatcttgtc tatcacaaag
     4441 ctttacaaag aggcgttggc actgacgtgg ccgtatgatc cgaagatcgg cccgtttgcc
     4501 gaacgcgacg cgaagtccta atggctgatc tcgcccaagt cataagacag attcctctct
     4561 tctcggcgtt gtcgcgagaa gatatcgcca aagtcttggg caagatggaa gaacgttact
     4621 acgtcagcgg cagaatcatt ttttcacaag gtgatcaggg cgatgccttc tacctcattc
     4681 aaaccggagc tgtccaggtc gtgcttgaaa accgcggtag agcggagatc attcgtgttc
     4741 tcggaccgca agactggttc ggcgaaatgg cgctgttatc cggcgagccg cgttcggcaa
     4801 ccatcgtcgc actcagagac tctttgatat ggcgactgag tcgccatgct tgggacgaat
     4861 tgatcgacaa acatccgacc tggctgctgc atttctgcgc cgctttgagt aaaaagctct
     4921 cttacgtcga acagcagtat tctcaagggc gcgaagcttt ctactcactg gcagaggaat
     4981 tctacagctc gaggccggca ccggaacaag cgttgctgcg ccggctttcg ctactcaccg
     5041 ttgccgaact gcaggcggct gaggaagtgc ttcccacccg gcttgcaagc cagtgcttgg
     5101 cgacgctgag agaaaatcac ttccctctgg ttcgggtaat cgattcgacg cgcctcgacc
     5161 tgcatccgtt tttcttagat ttcttgcgcg agaaattcgc cgagcatgag ggtcccgagg
     5221 acgctaagca ggtgcattgc gaattggccg agcggcttga aaagcttggc cgatggcagg
     5281 aggcgatcca tcattgtctg gccgccgaaa actggtcgag ggcggcggcg ttgttaagtc
     5341 gacacagcca ggtcttgctc gatggctccg ccgctgccgt cagagaggca atcgagcaat
     5401 tgccacgaga ccaaatcgat ctcgatctta tgcacatgaa agctgaagcc ctcgtgcggt
     5461 tgggcgagat tgaggccgcc gtggcctatt gcacagaggc actactggac tcagccaaaa
     5521 ccccgcgtga caaagccacg gcgacccgcc tgcatcatgc gatcagtagt cttgcgcgcc
     5581 aaggcgacca cgctcaggct ttgaatctct tgcgcaacgc tttggaactg cggcagcagg
     5641 acgcggtgtc cgacctggca agcatcgacg agaaattcga caccgccggg attgccgcgc
     5701 cgctacgtca gactcacaca gaattcacgg aaacgaacac cttaggcgca tcgaagtatt
     5761 ttccaggtgc gctggataaa tccaatctga gccgctggac cggtgccctg ctggggattg
     5821 tcgcatggag ttatctctgg ttggctgacc cggatatcgg tttggagcca agcgctacca
     5881 ggcaattggc cttgcttgtt ttgacgctaa ttttttggat gtttcgggtt tttccggaat
     5941 atggtgtcgc cttgctattt gccatcggca tgatcatttc cggcttagcg cgacccgaag
     6001 tggtcctcag tggcttttcc agcagttcgt ggttcatgac tctcggggtt cttggattgg
     6061 gcgcggccat caccagttcg gggctcttct accggctctc gctgcaactg gtgcgggtct
     6121 ttccgcttgg ctataggtgg caaatcgttg ccatggggtt catgggaatc gtcgtcatgg
     6181 cattgattcc gcagcagagc gctcgcacgg cgatcatcag ccagatgctg atgaacttat
     6241 ccgagagtct tagttataaa aatccgtcca aggcttccac cggtcttttc gtcgcgagct
     6301 tcctgggtct cggccaactc ggttttctat ttctgaccgg ttcaaccacg agcctgatcg
     6361 catggggcct tctgcccgag gaggttcgcg cccaatttag ctggggatac tggttctatg
     6421 ccgcgtcggt gccgaccttc acggtagtcg caattgttat ctcagctacc ctaattctct
     6481 acaagcccga gtctcaggcg cgtgtttctt acaagatggt tcaaacccag ctgcagattc
     6541 tcgggccgct gtcgcgccat gaatggatta ccctgggcgt tttgtgtttg acgttgggcg
     6601 gttggctcag caggtcgtat cacaatatcg acggcgcctg ggtcgcgctg atcgcgctgt
     6661 gtgtcttggt caataccggc gtgctcggtt ggggcatgat gaaaaagggc atcgattggg
     6721 aaatgctcct ctatatgggc gcgaccctga gcattcccgc gctgcttact caagcgaaga
     6781 tcgaccagtg gctggtcggc atgtttgctc cggtaatcct gccgtttacg aattggccgg
     6841 cgctctgttt caccgtcatc atagccatcg caattgcagt caagctggcc tttaccagct
     6901 tcttgacggt cgtgactttg acggtggcgc tcttgccact ggcatccgag ttgggcatca
     6961 gcccatgggt gatggcaatg atcgttctca tgggctcgga ggtttggttc cttagatttc
     7021 aggtggactg gcataccatg gcaagcgcga cgaccggagg aaaaggattt agctatccta
     7081 tgatgtaccg catcaatccg gtgtacgctg tcgcctatat attggccgcc atcgtcgcga
     7141 tcccctggtg gcgccacctg ggcttgatag gttgaactgg gcgcgccgga cacgcaccga
     7201 ccgtcaagcc ttcaacagcc gcactgccaa ca
//
LOCUS       DASUTG010000227         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1371376, whole genome shotgun sequence.
ACCESSION   DASUTG010000227 DASUTG010000000
VERSION     DASUTG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371376"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF089_05040"
     CDS             <1..228
                     /locus_tag="VF089_05040"
                     /inference="COORDINATES: protein motif:HMM:NF036542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HEX6173358.1"
                     /translation="GAKMLVKEPAARDFIADAFAHMKFVGYTESAVTVMMYAGVPETR
                     DKGFIALKGQDDCSTFVEACRQLRFWDRANM"
     gene            complement(583..1200)
                     /locus_tag="VF089_05045"
     CDS             complement(583..1200)
                     /locus_tag="VF089_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6173359.1"
                     /translation="MATTCPAAFNVDRLRQLVRAEYDRVAREPDGKFHFHRGPHYAAA
                     MLRYDAQELATIPEESTACFAGVGNPHRIGPIATGDTVLDVGCGAGMDLLLAAKRTGA
                     MGHAIGIDMTPAMIERAKAAALKAGLWKTVKIRRGVAEQLPIESETIDVVISNGVLNL
                     SADKVRAFSEIFRVLRPGGRLYLADVVVQRELSLEARSDVDLWAA"
     gene            complement(1381..2019)
                     /locus_tag="VF089_05050"
     CDS             complement(1381..2019)
                     /locus_tag="VF089_05050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6173360.1"
                     /translation="MITIKKVATAEQDTAVRAIVEAFRADPAARWMYPDSEQYWQHFP
                     SFVRAFAGAAFEHGSANLVDGYLAAALWLPPGIHPDEEAMSKLIRTTVPENDQANVFA
                     IFEQMDRYHPTEPHWYLPMIGVDPAHQAKGYGSALLQHALARCDREDKLAYLESTSPS
                     SVRLYQRHGFELLGTIQVGSSPRLSPMIRKPRAMWRGAGTNLPSNHSSTGWV"
     gene            complement(2132..2701)
                     /locus_tag="VF089_05055"
     CDS             complement(2132..2701)
                     /locus_tag="VF089_05055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6173361.1"
                     /translation="MSALEAKKIGTVNGLDVDALHDVIDEVKKDPSKGIVEFNVKTGW
                     KGQTRSETAVDSYKLGGKEIKRRFTINADEPIELLGENTSANPQELLMAALNACITVG
                     YVAGAAVNGITLNRLEIETTGRLDLRGFLGLDPKIKPGYESLQYVVRIAGNGTPEQFK
                     EIHENVMRTSPNYFNLSQPVKLEAKLEVG"
     gene            complement(2752..3663)
                     /locus_tag="VF089_05060"
     CDS             complement(2752..3663)
                     /locus_tag="VF089_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173362.1"
                     /translation="MQHNYSYETTLAISERIGWKVDDLIGGDKKFDFTKPFMPESLAQ
                     IKQLSFLTPQEQLTLNQIRGHEYLSMFGLVEEFILPYVVDHARPQLSGDDYRVRALLQ
                     FAGEEAKHIHLFKRFRQEFEEGFGSKCDFIGPAEDVSRFVLSHSPLGAGIAILHIEWI
                     TLRHYIEGVRDTQELDGQFKNLLKHHWLEESQHTKLDTLMVEEVVRNATAKEIDQAFQ
                     EYAEIGAFLDQGIQQQAEFDLESFVQATGRDLSRSEREQVKSAVLKGMRWTYLGTGMT
                     HPNFLATVAAIKPEARKQIEEMAPAFC"
     gene            complement(3959..5911)
                     /locus_tag="VF089_05065"
     CDS             complement(3959..5911)
                     /locus_tag="VF089_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6173363.1"
                     /translation="MINQDPEISPLAELDEESALRTILEGTANETGERFFAALVENLA
                     LALNTHGAWVTEYVQECRRLRALAFWMDGQWIQDYEVDIAGTPCERVIDTADLVHFPD
                     NLLQLFPDDPDITAINAVSYLGMPLKDVDGKILGHLAVIDRRPIPEEPKVQALFRIFA
                     ARAAAELQRQSVEKQVREREEKLRRLFDSAMDAIIELDQNLKVTRVNPAAEQAFQCPG
                     RQAIGDEFSRFVSNKSYQKILQLIDEFKPCPPERRSAWVPGGLRAVAREGDEFPAEAT
                     ISCFEIGRETFYTVILRNINERLEADRKINFLTAEAEYLREALNKLGGFDQIIGQSSA
                     LKTVLEDVEQVAVGDTTVLVLGETGTGKELIARAIHGASRRADKPLITINCAAIPAAL
                     MESEFFGHEKGAFTGATQTREGCFALADGGTIFLDEVGELSLDLQAKLLRVLQEGEFT
                     PVGGSHLRKVNVRVIAATNRDLKQAIRDGRFREDLYYRLNVFPISVPPLRERGEDAVI
                     LASEFAARFAQRMGKRIEPLSQEIKRRLKAYSWPGNVRELQNVIERAVITSRDGSINL
                     DRALPEIAQDVAHEIVSAVDGAMQPARRILPISELQQLERENILRALESSNWRVAGRD
                     GAASMLGINPSTLNSRIRALGIQRPR"
     gene            complement(6089..7015)
                     /locus_tag="VF089_05070"
     CDS             complement(6089..7015)
                     /locus_tag="VF089_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6173364.1"
                     /translation="MRLQIISTWAYLDFPHPGARMSLLAEITAIFSAMGWASDSVLVR
                     LGLRRSNIFAAMLMSFAVSTACMWTYLIATTSLEFLSSPAMIYFVISGCVQPLLARAL
                     FYEGITRIGVARAGPLRGTEPFFATIIGVSFLHEQPGALVYLGTILIMTSLWLISGQR
                     DGAAKWRLLDTAFPISAALISAISQSLRKQGLKILPDPFVAVAVVTTVSLVLFLIFVL
                     TTGRAQSLRMERQGFSFFLAAALLATAAQVLNFVALGRGELSVIIPLLNTTPLFTVLL
                     SVLFLRKVETVNRRIVFGALLMFAGIVTITSR"
     gene            7129..>7563
                     /locus_tag="VF089_05075"
     CDS             7129..>7563
                     /locus_tag="VF089_05075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173365.1"
                     /translation="MENGKQPEAKVVKLRTQLVKEGHTRNLLAETDLMTLRIHCYAPG
                     LGENALHSHTKEDHIFLVLEGPAEFNTGRDGKTVLNASKHQAIVLPAGCYYQFRNSGD
                     TPLVMTRIGAGPDKQDQRLNPDGTPIPGRTHEQGAAKPVLIEG"
BASE COUNT         1715 a         2109 c         2049 g         1690 t
ORIGIN      
        1 ggcgcaaaaa tgcttgtgaa agaaccggcg gcgcgcgact tcatcgccga tgcttttgcg
       61 cacatgaagt ttgtcggtta cacagaaagc gcggtcactg taatgatgta cgccggagta
      121 cccgaaaccc gggacaaggg atttatcgcc ctcaaaggac aggatgattg ttcaaccttt
      181 gttgaagctt gtcggcagct gcgcttttgg gaccgcgcca acatgtaatc atccctcagc
      241 atggggtggt ctctgaacag gaagcttagc atgacctttg gctacccaca gctctgtact
      301 cccgttatat accggcaaga ataacgggag tatgtctaaa acctagatta cgatcagtaa
      361 tcatgtttag ggctggtctg ttgttgatcg tgctgcggat tctaaatagc tgagaggttt
      421 gcgagcgaag aagttcaccc cctgcacgta gaggtctttg gacagcttga ccttggcgga
      481 ggtgccttcg taacaatcga agcgctcgac gatcttacca tcggcgaaac ccatctgagc
      541 agttagctgg taaagctccg cctccggcag agccccgccg attcaggcgg cccagagatc
      601 gacatcgctg cgcgcctcga gggacagctc tcgctgtacg accacatcgg ccagatacaa
      661 gcggccaccc gggcgcagca cgcgaaagat ttcgctgaat gcccgtactt tatcagccga
      721 caagttcagc acgccgttgg aaatgacaac gtcaattgtc tcactttcta ttggcaattg
      781 ttcggcgacg ccgcgtcgga ttttcacggt tttccaaagc ccggccttta gcgccgccgc
      841 tttggcgcgc tcgatcatgg cgggtgtcat gtcgatgccg atggcgtgcc ccatggctcc
      901 tgttcgcttg gccgccagga gcaagtccat gccggcgccg cagccaacat ccagcactgt
      961 gtctccagtc gcgatcggac caattcgatg cggattgccg acaccggcga aacaagcggt
     1021 agactcttcc gggatcgtcg ctaactcttg cgcgtcgtat cgcaacatgg ccgcggcgta
     1081 gtgcgggccg cgatggaagt gaaacttacc gtccggttcg cgggcgactc ggtcatactc
     1141 cgctcggact agctgtcgca acctatcgac gttgaaggcg gccgggcatg ttgtcgccat
     1201 ggtcttctct cctaataagt ttaaaatagc gctgccttgc cgttcgaact ctgtcgatca
     1261 aacagggctg cttctggtct ttgtgcgaga accggcgtct ctctcctgaa ggagtgggaa
     1321 gagagatggc gcagtagacc gcggcaagcg caaagaataa gaatgcttca acactcatag
     1381 ctagacccat cctgtggatg aatgattact cggtaggttt gttccggcgc cacgccacat
     1441 tgcgcgcggc ttccgtatca tcggagacaa ccgcggcgag gagccaacct gaatcgtgcc
     1501 gagtaactca aagccgtgtc tctgataaag ccggacgctg ctcgggctgg tagattccag
     1561 ataggcgagt ttgtcttcgc gatcgcagcg cgcaagcgca tgttgcagca gcgccgagcc
     1621 atagcctttg gcctggtgtg caggatcgac accaatcatt ggcagatacc agtgtggttc
     1681 ggtgggatgg taacgatcca tctgctcgaa tattgcgaag acgtttgcct gatcgttttc
     1741 cggtaccgtc gtccgaatca gtttgctcat ggcttcttca tccggatgaa tccccggagg
     1801 aagccaaagc gctgcagcca agtagccgtc caccagattg gcgcttccat gctcgaacgc
     1861 ggcgccggca aacgctctga cgaaactcgg aaaatgctgc caatactgct ctgaatcagg
     1921 atacatccag cgggcggcag ggtcggccct aaaggcctcc actatggccc tgacggcggt
     1981 atcctgttcc gcggttgcga cctttttgat cgtgatcata gcttctctcc gatttgtggg
     2041 ggcgcagaag gggcaccaag ttgattaagc agttgtaaat atcttgtggt ctaggtgcct
     2101 gcccttcgcg ccgtcctggt tataacttcc gtcaaccgac ctcgagcttg gcctcaagct
     2161 tcacaggctg cgacagattg aaatagttcg gcgatgttct catgacattc tcatgaattt
     2221 ccttgaactg ctccggcgtg ccattgccgg caatccgaac cacgtactgc agtgattcgt
     2281 agccgggttt aatctttgga tcgagcccga gaaaaccacg aagatccaga cgacccgtgg
     2341 tctcgatttc cagcctgttc agagttatcc cattgaccgc cgcgcccgcc acatagccga
     2401 ccgtgataca ggcattgagc gccgccatta gcaactcctg cggattggca gaggtgttct
     2461 cgcccaatag ctctatgggt tcatccgcgt tgatcgtaaa gcgacgtttg atttccttgc
     2521 cgccgagctt gtacgagtcg accgcagttt cagaacgggt ttgtcctttc cacccggttt
     2581 tgacattgaa ttcaacgatg ccttttgacg gatccttctt gacctcgtca atcacgtcat
     2641 gcaacgcatc gacatcaagc ccgtttacag tgcctatttt ttttgcttca agtgcagaca
     2701 tatttgactc cttttccttt taattttgtc tttgttattt tgttgcgggc atcagcaaaa
     2761 ggctggcgcc atttcctcga tctgcttgcg cgcctcaggc ttaatagccg ccactgtggc
     2821 gagaaagttc ggatgggtca tgccggtacc gaggtaggtc cagcgcattc ctttgagcac
     2881 ggcactcttg acctgctcgc gctcgctccg ggacaaatcc cttccggtcg cttgcacgaa
     2941 actctcgaga tcgaactcgg cctgctgctg gatgccctga tctaagaaag ctccgatctc
     3001 tgcgtactcc tgaaaagcct ggtcgatctc cttcgccgtg gcattgcgca cgacttcctc
     3061 aaccatcagc gtatcgagct tggtatgttg cgactcttcc agccaatgat gcttaagcag
     3121 gttcttgaac tgcccatcaa gttcctgggt gtcgcgcacg ccttcgatgt agtgcctgag
     3181 tgtgatccat tcgatgtgga ggatcgcgat gccagctccg aggggactgt gggatagcac
     3241 gaaacgcgag acatcctccg ccggtccgat aaagtcgcac ttgctgccga agccttcctc
     3301 aaactcctgg cgaaagcgtt tgaacaggtg aatgtgtttg gcctcctccc cggcgaattg
     3361 aagaagggcg cgcacccggt agtcatcacc ggaaagctgc ggacgggcat gatcgaccac
     3421 gtaaggcaga atgaactctt cgaccaggcc gaacatcgaa agatactcgt gaccgcggat
     3481 ctgattgagg gtcagttgct cttgcggagt aagaaaagaa agctgcttta tctgagccag
     3541 cgactcaggc atgaagggtt tggtgaagtc gaactttttg tcgccgccga tgagatcgtc
     3601 caccttccag ccgatcctct cggatatggc cagtgttgtt tcataagagt agttgtgttg
     3661 catgtttcac ctcctcgaaa agtttgttag ctcgccatag agcagctagt gtgccagcag
     3721 cgttaactgc caatttccga atgattgccg ttagttaggg aattgcgcgg agcagggcta
     3781 aatgcgagat ctcacgagtc gtgagatctc acatcgctgg ttgtgagatc tcacatatta
     3841 aacggggctc acgtgaaaag tgcgagccga aagagatctt cctcacgcga aaaagcagaa
     3901 gaatataaat gatcggcagt aatttatctc tagagtcccg ccgagcgcat ggcaagtctc
     3961 atctcggccg ctggatgccg agagcgcgaa tgcgggaatt gagagtagaa ggattgatgc
     4021 cgagcatact ggccgcgccg tctctgccgg cgacacgcca gtttgatgat tcgagagcgc
     4081 gcagaatgtt ctctcgttcc aactgctgaa gctcactaat aggcagaatt cgccgcgccg
     4141 gctgcatcgc gccgtccaca gccgatacaa tttcatgagc gacgtcttgg gcgatttccg
     4201 gcagagctcg gtcgagattg atagatccat cgcgggaggt aatcaccgcc cgttcgatca
     4261 cattctgcag ctcacgcaca tttcccggcc aggaataagc cttcaaccgt ctcttgatct
     4321 cttgtgaaag aggctcgatg cgcttgccca tacgttgcgc gaatctggcg gcaaactctg
     4381 acgccaggat cacagcatcc tcacctctct cgcgcaatgg cggcacactg attggaaaga
     4441 cgttcaaacg ataataaaga tcctcccgaa aacgcccgtc gcgtatcgcc tgtttgagat
     4501 cgcggttggt tgccgcgatg acacgcacat tcactttgcg cagatgagaa ccgccgacgg
     4561 gtgtgaattc gccttcctgc aaaacccgca acagcttggc ctgtaaatcg agcgaaagct
     4621 cgcccacctc atcgaggaaa atggtgccgc catcggccaa ggcaaaacat ccctcgcgtg
     4681 tctgcgtcgc gccggtaaag gcgcccttct catgcccgaa aaattcgctc tccatgagcg
     4741 ctgctgggat cgcggcgcag ttaatcgtga tcaggggctt gtcggcgcgg cggctggcgc
     4801 cgtgaatcgc cctggcgatc aattctttgc cggtgccggt ttcgccaaga accaggactg
     4861 tggtgtcgcc gacggcaact tgctcaacat cctcgagcac cgttttgagc gcggagcttt
     4921 gtccgataat ttggtcaaag ccgcctagct tgttgagcgc ttcacggaga tattccgctt
     4981 ccgcggtgag aaagtttatc tttcggtcgg cttcgagccg ctcattgatg ttccgtaaga
     5041 taactgtgta gaaggtttcc ctgccgattt cgaagcacga gatggtggct tctgccggaa
     5101 actcatcacc ctcgcgcgcc actgcgcgta atccgcctgg aacccatgca gagcgtcgtt
     5161 ccggcgggca cggcttgaac tcatcgatga gttgtaagat cttttggtaa ctcttgttgg
     5221 aaacgaaccg gctgaattca tcgccaatcg cctgccggcc cgggcattga aacgcctgct
     5281 cggctgccgg gttcacccgg gtcaccttga gattctggtc aagctcgatg atcgcatcca
     5341 tggcgctgtc aaacaggcga cgtagcttct cctcgcgctc tcgcacctgc ttttcgacac
     5401 tctggcgttg caactcggcc gctgctctgg cggcaaaaat tctaaataga gcctgaacct
     5461 tgggttcttc cggaattggc cgtcgatcga tcaccgcaag atgaccgaga attttgccat
     5521 cgacgtcttt caaaggcatt cccaaatagc tcaccgcgtt aatcgctgtg atgtcaggat
     5581 catctggaaa caattgtagg aggttatcgg gaaaatgaac caggtcggcg gtgtcgatca
     5641 ctctttcgca aggcgtcccc gctatatcga cctcatagtc ttgtatccac tgcccatcca
     5701 tccaaaatgc aagagcgcga agccggcggc attcctggac atattcagtg acccaagcgc
     5761 cgtgggtgtt taacgccagc gccaggtttt cgaccagcgc ggcaaaaaac cgctcgccgg
     5821 tctcatttgc ggttccttca agtatcgtcc gcagcgcgga ctcttcatcc agctcggcga
     5881 gcggggatat ttcggggtct tgattgatca taaagcacct cggcgccctt cgaattaagt
     5941 tttgcggcct ggacccctcg tcctcagaac agccgtaata tccgggaccg atttgcctgt
     6001 gtcaagcgcc actgccggtt ctagcagcgc tcaatcggct ggcgcatagc aaaaaccagc
     6061 tccagacggt tataacctca gcgacgtatc accggctggt aattgtcacg atccctgcga
     6121 acataagaag tgcgccgaaa acgatgcgcc ggttgaccgt ttccactttg cgcaggaaaa
     6181 gaacgctcaa gaggacggta aacagtggtg tggtgttcaa gagcggaata atcaccgaca
     6241 actcgcctcg tcctaaagcg acaaaattga ggacttgagc cgcagttgcc aggagcgccg
     6301 cggcgagaaa aaatgaaaag ccctggcgct ccatccgcag cgactgggct ctgccggtag
     6361 tcagcacaaa gattaaaaat agcaccagcg agaccgtggt gactacggct accgcaacaa
     6421 atggatcggg tagaatcttc agtccttgct tgcgcaagct ttgcgagatc gcggaaatca
     6481 acgctgcgct gatcgggaaa gcggtgtcga ggaggcgcca cttggccgca ccatcccgct
     6541 ggccggagat caaccagaga cttgtcataa tcagtatcgt gccaagataa accagcgcac
     6601 cgggctgttc gtgcaaaaag ctcacgccaa taatcgtcgc aaaaaatggc tcagtgccgc
     6661 gcagcggacc agcgcgagcg acgccgattc gggtgattcc ttcatagaat agggctcgcg
     6721 ccagcaacgg ttgcacgcaa ccgcttatga caaaataaat catcgccggc gaggacagga
     6781 actcaagcga ggtggtggcg attagatacg tccacatgca ggcagtggag accgcgaagc
     6841 tcatcaacat cgctgcgaaa atattggatc gccgtaagcc tagccggacc agaacagaat
     6901 cggaggccca gcccatggcg gagaaaatag ccgtgatttc agcaagtaga gacatgcgcg
     6961 cgccaggatg tgggaaatcc agataagccc acgtgctgat tatttgcaac cgcaaaaatt
     7021 cctcacgaag cggcgcgttt ttaatcgatc gtgtattgca cagtcccgac gacctgacct
     7081 agaatcgtgt ccggagccag gaacaaccaa aaactaacag gaggggttat ggaaaatggc
     7141 aaacaaccgg aagcaaaggt ggtaaaactg cgaactcaac tggtaaagga ggggcacacg
     7201 cgcaacctgc tggcggaaac agatttgatg acattgcgta tccattgcta cgccccgggc
     7261 ctcggcgaga acgcgctcca ctcccacacc aaagaggatc atattttcct ggttcttgaa
     7321 ggacccgctg aattcaatac cggtcgtgac ggcaaaaccg ttctcaatgc gagcaagcat
     7381 caagcgatcg ttcttccagc gggttgctac taccagtttc gcaattccgg agatacccct
     7441 ctggtgatga ctcgaatcgg cgccggtcca gataaacagg atcagcgtct gaacccggac
     7501 ggcacaccga ttcctggaag aactcacgag caaggcgcgg caaagccggt gctgattgaa
     7561 gga
//
LOCUS       DASUTG010000228         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_926613, whole genome shotgun sequence.
ACCESSION   DASUTG010000228 DASUTG010000000
VERSION     DASUTG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926613"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF089_05080"
     CDS             <1..367
                     /locus_tag="VF089_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173366.1"
                     /translation="GPQRIFAELNGKRLEFLKESLPKLSQVALLVSGNFPGNAIDLKE
                     TEAAARSMQLQTHFMEVRDASKLEAVFKTMIRRRIDGATVFPGQPTLFANRKLGGGPR
                     RQISSPRNYPLADYVTAAD"
     gene            367..570
                     /locus_tag="VF089_05085"
     CDS             367..570
                     /locus_tag="VF089_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6173367.1"
                     /translation="MSYGVNNLDLFRRADSYVDKILKGAKPGDLPVEQPTKFDLVINL
                     QTAKQIGVTIPPGVLARADKVIK"
     gene            631..2253
                     /locus_tag="VF089_05090"
     CDS             631..2253
                     /locus_tag="VF089_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173368.1"
                     /translation="MKLNKLFLFGILVTLFCPRLVMAQEKAAEKLGQVNFPVTCTAAA
                     QKQFDQALAALHSFWYEEALRVFTAVAETDPGCAMAYWGIAMSIYYPLWVPPSQPTLQ
                     KGISAIEKARSIGAKTEREGAYIAAIESFYKDSDKLEHRARALAYEKAMEQIHRQHPS
                     DKEAAIFYALALNATATPTDKTYAQQRKAGEILEKVFAEQPDHPGVAHYIIHSYDNPS
                     TANRGLPAAQRYIKIAPSVPHAQHMPSHIFTRLGLWQESIQSNLGAVAASKEYAAKVH
                     PGAAYYEHLHALDYLAYAYLQGAQDQEAKRVLDELRAIKKVQPQAFQAAYAYAAIPAR
                     YALERRRWSEAAALTVQPSAFPWSRFQWAEAVTHFARAMGSARSGDVVSSRKDIEKLE
                     SLQDALVKAKDSYWAKQVDIQRRVASAWYLRAENKNDEALQLMESAADLEDSTDKHPV
                     TPAPIQPARELLGEMLLELGQPAQALKEFEVSHRVEPNRFRGLYGAAKAAQESGDREK
                     ARLYYAKLISLCERADADRPELKDAKAFLSDK"
     gene            2474..3472
                     /locus_tag="VF089_05095"
     CDS             2474..3472
                     /locus_tag="VF089_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173369.1"
                     /translation="MTVIKIVSCLLLTAFLLPMSTADAQQPHKIYKVGRLSAGSAKDP
                     LTNATYDAFREGMRDLGWIEGSNIIFENRWAGEPNASAVALAAELVRLKVDVIVAVGS
                     PLIQAAKQATSAIPIVMSGTGADPVAAGFVASLGRPGRNITGLSMLSTELSGKRLELL
                     KETVSSLGRVAVLRNRKFPAVAIQSKETEEAAKSLGLQLQAWEVRNPQEIDSAFTAMS
                     KAATSGLIVFSDPVLLERNRSSIITLARKYRIPAVYPWRNYVEEGGLASYSANLLDMH
                     RRAATYVDKILRGAKPAELPVEQPTKFELVINLKTAKQLGLAVPQSVLARADKVIR"
     gene            3469..>3972
                     /locus_tag="VF089_05100"
     CDS             3469..>3972
                     /locus_tag="VF089_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6173370.1"
                     /translation="MTFWIFDFRSWIDIQGNLPLSGFQSESFSGNRKSAIQNPKWLGL
                     SVFTFMVLVSGAAAQAQQTAAVPRIGVLHTVSSSSVAARTQALRQGLRELGYVEGKTI
                     TAEFRFAEGKLDRVSSLAAELVRLKPDAIVTSGPASTRAAKEATVRIPIVMARDIDPV
                     GNGFVASL"
BASE COUNT          956 a         1066 c         1111 g          839 t
ORIGIN      
        1 cgggcctcag cgcatattcg ccgaactcaa tggaaaacga ttggaatttt taaaggaatc
       61 gcttcctaag ctatctcaag tggcgcttct cgtgagtggg aatttcccag gaaacgcgat
      121 cgatttgaag gagaccgagg cggcggcccg ttcaatgcag ctccagaccc acttcatgga
      181 agtcagagat gcgtctaagt tggaggctgt ttttaagacc atgataagaa gacgcataga
      241 cggagcgact gtctttccag gccagccgac actatttgcg aatcgcaagc taggcggtgg
      301 ccctcgccgg caaatatcgt ctccccgtaa ttatccgctc gcggattatg tgacggcggc
      361 ggactaatgt cttacggagt aaacaatctc gacttatttc ggcgtgctga ttcctacgta
      421 gacaagattc tcaagggcgc taagcccggc gacttgcccg tcgaacagcc gacgaagttt
      481 gatctcgtga ttaatcttca aaccgcaaag cagatcggcg tgacgattcc gccgggcgtg
      541 ctggcgcggg cagacaaggt gatcaaatga ggcgggtgta agggtgaggc gagatgaagg
      601 cgctaccgta agaacgtgaa ggaggcgttt atgaagctca acaaactttt tctgtttggg
      661 attctcgtca cacttttttg cccgcgctta gtaatggcgc aggaaaaggc ggcggaaaaa
      721 ctcggccagg ttaacttccc ggtcacctgc accgcggcgg cccaaaagca gttcgatcag
      781 gctctcgctg ccctacattc cttttggtat gaggaggcgc tcagggtgtt taccgcggtt
      841 gcggaaacgg accctggttg cgcgatggcc tactggggaa ttgccatgag tatatactat
      901 cctctttggg tgccgccgag tcagccgaca ttgcagaaag ggattagcgc catcgaaaag
      961 gcgaggtcga tcggcgcaaa aaccgaacgt gaaggcgctt atatcgcggc aatagagagc
     1021 ttctataagg actcagacaa gctcgaacac cgagcccggg ctctcgcgta cgaaaaagcg
     1081 atggagcaga tccatcgcca gcacccaagc gacaaagaag cggcgatctt ctacgcactg
     1141 gcgctcaacg ccacggcaac tccaaccgac aagacttacg cacagcagcg aaaggccggg
     1201 gaaatccttg aaaaggtctt tgcggagcag cccgatcacc cgggcgtagc tcactacatc
     1261 attcacagct atgataatcc gtccacagca aaccgtgggc ttcctgctgc gcaacgctat
     1321 atcaagatcg ctccctcggt tcctcacgcg cagcacatgc cttcgcacat ttttacgcgg
     1381 ctcggtttgt ggcaagaatc gattcaatca aatctcgggg ctgtggccgc atcgaaagag
     1441 tatgcggcta aggttcatcc cggagccgca tactatgagc atcttcatgc cttggattac
     1501 ctggcgtatg cctacctgca aggagctcag gatcaggaag ccaagcgcgt ccttgacgaa
     1561 ctgcgcgcga ttaaaaaagt ccagccgcaa gccttccagg ctgcttacgc ctatgctgcc
     1621 attccggcgc gttatgcgct ggagaggcgc cgctggtctg aggccgcagc gttaaccgtg
     1681 caaccaagcg cctttccctg gagccgtttt cagtgggccg aagccgttac acacttcgcg
     1741 cgcgccatgg gatctgcacg cagcggcgat gttgtcagca gtcgaaaaga catcgaaaaa
     1801 ctcgagtcgc tccaagatgc cttagtcaag gccaaggaca gctactgggc taagcaggtc
     1861 gacatccaac gccgggtggc aagcgcctgg tatttacggg ccgaaaataa aaatgatgag
     1921 gcactacagc tcatggagtc cgcggcggac ctcgaggact caaccgacaa acacccggtc
     1981 acgcccgcgc cgatacagcc tgcgcgggaa ctcctgggtg aaatgcttct tgagctcggc
     2041 cagcctgccc aagcattgaa agaattcgag gtttcgcacc gagtggaacc taaccggttc
     2101 cgcggcctct acggcgcggc gaaggcagct caggaatctg gcgaccgcga gaaagcgcgc
     2161 ctttactatg caaaactaat ttcgctctgt gaacgcgctg atgccgatcg gcccgaactc
     2221 aaggatgcta aggcattctt gtcggataaa tgaaccaaat ttaggcgcag gcctgcattg
     2281 atatcacgaa cctaggctca aacatgtacg agcccatttt gtactttcgg atcgactagt
     2341 ttctgtgcca tcttcgacct gtgactaatt cgaactgcga aaaagcagtt gatcttgggt
     2401 gttgaagagc gaaaagagat gaactgagcc gtgaatcgct aaggagagtc gtaaaacaat
     2461 cgaaatcaaa gcgatgactg taatcaaaat cgttagctgc ctactgctca ctgcgtttct
     2521 gcttcctatg tccacggccg atgcacagca gccgcacaag atttacaaag tcggaaggct
     2581 tagcgcaggc tctgctaagg atccgcttac caatgcgacc tacgacgcct tccgcgaagg
     2641 gatgcgtgac ctaggttgga tcgagggcag caatatcatc ttcgagaatc gttgggcggg
     2701 agagcccaac gcgagtgcgg ttgctctcgc agccgaactc gttcggctca aagtggacgt
     2761 catcgtcgcc gtcggtagcc cgttaattca agcagctaag caggcaacaa gcgcaattcc
     2821 catcgttatg tccggcaccg gagctgaccc ggtcgcagcg ggtttcgtcg ccagtctcgg
     2881 gcggccggga cgcaacatca ccggattgag catgctttcc accgagctaa gcggtaaacg
     2941 gttggagctg ctcaaagaga cggtgtccag tcttggacgt gtcgccgtcc tgcgcaaccg
     3001 aaaatttccg gctgtggcga ttcagtcaaa agaaacagag gaagctgcaa agtccttggg
     3061 acttcagctc caagcctggg aagtgcgcaa cccgcaggaa attgacagcg ccttcactgc
     3121 tatgagcaag gctgcaacga gcgggctcat tgtctttagc gatcctgtgc tactcgagag
     3181 aaatcgttca tcgatcatca cgctggcgcg caagtaccgc attccggctg tttatccttg
     3241 gagaaactat gtcgaggagg gcggcttggc aagctattcg gcgaatctgt tggacatgca
     3301 ccgccgggcg gcgacctacg tcgacaaaat acttagaggc gccaagcctg ccgaacttcc
     3361 ggttgagcag ccgaccaagt ttgagctggt gatcaacttg aaaactgcca aacaactcgg
     3421 attggcggta ccgcagtcag tgctggcacg agcagataag gtcatcagat gacattttgg
     3481 attttcgatt ttagatcttg gattgatatc cagggaaact tacccttgag tggatttcag
     3541 tcggagtctt tttccggtaa tcgaaaatcg gcaatccaaa atccaaaatg gctggggctt
     3601 tcggtattta ctttcatggt cctggtaagt ggggctgcgg ctcaggcgca gcagacggcg
     3661 gcagtccccc ggataggtgt tctacatacg gtttcctctt cctctgttgc ggcgcgtaca
     3721 caagcgttgc ggcagggact gagagaactc gggtatgtag agggcaagac aatcactgca
     3781 gaatttcggt tcgcagaggg aaaactagat cgtgtctcct cgttggcagc cgagttggtg
     3841 cgtcttaagc cagacgccat cgtcacaagc ggtccggcat caacccgcgc tgccaaggaa
     3901 gcaaccgtca ggattcccat tgtcatggcg cgcgatatcg accctgtcgg gaatggattc
     3961 gtcgctagcc tc
//
LOCUS       DASUTG010000229         5917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1667206, whole genome shotgun sequence.
ACCESSION   DASUTG010000229 DASUTG010000000
VERSION     DASUTG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667206"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1421
                     /locus_tag="VF089_05105"
     CDS             273..1421
                     /locus_tag="VF089_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173371.1"
                     /translation="MARDGYKIFDADTHVRPDAELLEPYMASSARDKLARYEKYRAKN
                     KDGAVTYLMGERSYKRRLGLSGDAAPQKDEYMSGYKRHQHGKPDPLCERDPAVRIKDM
                     DTEGIDVNLMLPSGWFGCWTTIDDVALECAVYEAYHRWMADYCAVFPERLKGVILVSA
                     RDVKTSVAELRRAAKESWPLAIFVYAPYQFPLDHPDLEPIWQAAAENDLSIALHTFTV
                     MPPYAPGGLDSWGNLWLQRSAAHPWCGQRNMAAIIGAGIMDRYPSIRVGTLEAGHGWL
                     PVWVSRLEEHTKLCPEALPPLKQTIRSYVTGGRYFQSIEVSEGAEITRSVIELLGPDI
                     LMFGSDYPHGESWFPVSAETVFGWNLTPDHLRKLLWDNAVKYYRRYSA"
     gene            1379..2335
                     /locus_tag="VF089_05110"
     CDS             1379..2335
                     /locus_tag="VF089_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX6173372.1"
                     /translation="MGQRSEILPAVFGMTPSKKLRRLLGRKNKVLAVLGAPNAFHAKI
                     MEANGVEACFVGTSITGGNYTGQPDLGILSMTECVEFAKWIARAVKIPVILDGDTGHG
                     GIMAVRRLVRECIDAGLAGLRLDDQPLDQKRRTGSAGISIVSREDAITRYRVAVDAKD
                     EIDRDFVIMAQCYARNALNGGMDELLRRLRFYETEGRVDWVQFESPHSVAEIKRARRV
                     VKGPFSAMKGQLSRALTLKEHAALGLNAAWYTFLPDLVLKTAAWSFFQEFKDKDMRAW
                     DDFAAAHSGTPFTGGGKLPWDATGMTELSKFEQNYAIKSKRR"
     gene            2339..3376
                     /locus_tag="VF089_05115"
     CDS             2339..3376
                     /locus_tag="VF089_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173373.1"
                     /translation="MNMQKTMLIILLLLFGPAKSSAQSDFFKGKQMKIVVGASAGAAS
                     DLYARAVANHLPKQIPGKPDIIVQNMPGGGSLTAANYVYSVAKPDGLTLGAVTAPIYF
                     AQLLGRKEVQFDWAKFTWIGTPEENDELLFVRSDQPYKNLLDLRKATEPLRCGASGVG
                     STGFYIPKLIEEIFGARITVVTGYPGAADVDLAVEKNEVHCRGTTISAFFGREPGRTW
                     AKNGFVRFLVQTGDRRNHRLPDTPTMWELMDQEKGTDATKRLARVILGPGAFGRPILA
                     TPGIPADRVKLLREAYVNMLKDPEFLAEAKKRQWEIAPVSGERLEALAKEVVNQPPEI
                     IERMKKVLGES"
     gene            3401..4273
                     /locus_tag="VF089_05120"
     CDS             3401..4273
                     /locus_tag="VF089_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173374.1"
                     /translation="MDLAKYIVKARPGVLGGKHEEKRPLRQLSALPVKRYVFINRDSH
                     PDADIYVAIHQAKDLPSPVPDYQVPHCHNTDEFYYFIGNHSDLTGLEGQIIFEGKVHK
                     IVSPACVYIPTGTVHEYKVTAGNGTVTVLFRNRGYTHEDKPFDVGKGERDFARYAGYI
                     FKPEVRPTTEIKYHTEAAPGTRYVFVDGKLKPEANFYTVVRSVAGVLPTQANYVDMHR
                     HNCDTQHIAIGTGPELTGLKIEFQIRDDKMVVESPVGVNIPAGTPHSQRIIEGAGHFF
                     NFVPKSFYNDGLMS"
     gene            4514..5584
                     /locus_tag="VF089_05125"
     CDS             4514..5584
                     /locus_tag="VF089_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173375.1"
                     /translation="MVRFQSSSVVFTVLVALLLASLAASPALSQAPFYQGKTITLIVG
                     AGPGGMGDLRAKALASVLAKNIPGNPSITFQYMPGAGGRKAANHLYNTARPDGLTLYR
                     ISSSIVSYGVLGETGVQYDVDKLIYLGTTEHQLYYVFMTRAALGHDSLTKLRAAAGLR
                     IGGAPVGHTGYLHSRMIAYFLDLGNSRIIPGYENEDLDVAFARGEVDAQVASMGTISQ
                     HDFLKNKAVHFHAGIEYPRGWKDPRFSHLNLPDLESFAKTESEKKLLAMMRNLRSVGT
                     ALLFPPGVPKDRVDILKEAVRKTHTDPAFIAEYRRLTSGDIPTPLMPDEQAKVVREIP
                     RDAETIELFKKFAGTSALPPRQ"
BASE COUNT         1321 a         1655 c         1723 g         1218 t
ORIGIN      
        1 gcgtacttac actacgtcgc agcgaggcga ttgagcggca acgaagcata tgggtctttt
       61 tcagcaggcc tgctagccac ccgcgattaa gtcattaacc cgtgcctagc acgaaaccgt
      121 gccgccggca aaaattcgaa agatccgcaa tcaacggatt tgatcagcac cttcattaag
      181 atcgccttcc ccctggcaaa aagagcttga caggccgcct gatttacggc taccgactgt
      241 gatggcgcta accaaaacga ggagtcaaac ccatggcacg cgatggctat aagatcttcg
      301 atgccgacac acatgtgcgg cccgatgcgg agctgttgga accgtatatg gcaagttccg
      361 ctcgcgataa gctggcgcgt tatgaaaagt atcgggccaa gaataaagac ggcgccgtga
      421 cctatttgat gggcgagcgc tcctacaagc gacgcttggg cttgagtggc gacgcagcgc
      481 cgcagaaaga cgaatacatg agcggctaca agcgccatca gcacggcaag cccgatccgc
      541 tctgtgagcg cgatcccgcc gttcggatca aggacatgga taccgagggc atcgatgtca
      601 atctcatgct gccttcgggt tggttcggtt gctggaccac catcgatgac gttgcgctcg
      661 agtgcgcggt ttacgaagcc tatcaccgct ggatggccga ttattgcgcg gtttttcccg
      721 aacgtttgaa gggggtgatc ctggtgtccg cccgcgacgt gaaaacttcg gtcgcggaat
      781 tgcgtcgcgc cgcgaaggag agctggccgc ttgccatatt tgtctatgcg ccgtatcagt
      841 tcccgctcga tcatcccgac ctcgaaccaa tctggcaagc cgcggcggaa aacgacttaa
      901 gcatcgctct acacaccttc acggttatgc cgccgtacgc gccaggcggt ctcgattcct
      961 ggggtaatct ctggctgcag cggtcggccg cgcacccctg gtgtggccag cgcaatatgg
     1021 ccgccatcat cggcgctggg atcatggacc gctatccgag tattcgcgtg ggcacattgg
     1081 aagcgggaca tggctggctg ccggtgtggg tgagtcggct ggaagagcac accaagctct
     1141 gtcccgaagc gttgccgccg ctcaaacaaa cgattcggag ctatgtcacc ggtggtcgtt
     1201 attttcagag tatcgaagtc tccgaaggcg cggaaattac ccgcagcgtg atcgaacttt
     1261 tagggccgga tattctcatg ttcggttcgg actaccccca cggcgagagc tggtttcccg
     1321 tttctgcgga aacggttttc ggctggaatt tgacgcctga tcatctgcgc aaactgttgt
     1381 gggacaacgc agtgaaatat taccggcggt attcggcatg acgccgagca agaaattacg
     1441 tcggctgctc ggccgaaaaa acaaagtttt ggcggtactt ggcgccccta acgcctttca
     1501 cgccaagatc atggaggcca acggcgttga agcctgcttc gtcggcacga gcatcacggg
     1561 cgggaattat acgggtcaac ccgatctcgg tattctgtcg atgacggagt gcgtggagtt
     1621 tgccaagtgg atcgcccgtg cggtcaagat cccggtcata ctcgacggcg ataccgggca
     1681 cggcggcatc atggcggtgc gccgcctggt ccgcgagtgc atcgacgcgg gactggccgg
     1741 gttacggctt gacgaccagc cgttggatca gaaacgacgc acgggatcgg cgggcatttc
     1801 catcgtcagc cgcgaagatg cgatcacgcg ctatcgtgta gcggtggacg ccaaggatga
     1861 gatcgatcgc gattttgtca ttatggcgca gtgctacgct cgcaacgcac ttaacggcgg
     1921 catggacgaa ttgttgcgcc ggctacgctt ttacgaaacc gaaggccgcg ttgattgggt
     1981 gcagttcgag tcgcctcact cggtggccga gatcaagcgc gcccgcagag ttgtcaaagg
     2041 gccgttctcg gcgatgaaag ggcagctatc gcgggcgctc acgctcaaag agcatgcagc
     2101 tctcgggctc aatgccgcat ggtacacctt tctacctgat ctggttctga aaacggcggc
     2161 ttggtctttt tttcaggagt tcaaagataa agacatgcgc gcctgggatg acttcgccgc
     2221 ggcgcatagc ggcaccccgt ttaccggcgg cggaaagttg ccctgggacg caaccggcat
     2281 gacggagttg agcaagttcg aacagaacta tgcgatcaaa tcgaaacgga gatagataat
     2341 gaacatgcaa aagacaatgt tgattatctt attgttgttg tttggaccgg ccaaaagcag
     2401 cgcgcagagc gattttttca agggcaagca aatgaaaatc gtcgtcggcg cctccgccgg
     2461 cgcagccagt gatctgtatg ctcgcgcggt ggcgaatcac ctgccgaaac aaatccccgg
     2521 caagccggat atcatcgtcc agaacatgcc gggtggcgga tcgctgacgg ctgcgaacta
     2581 tgtctatagc gtggccaagc cggacggcct tacattgggg gcggtgacag cgccgattta
     2641 tttcgcgcag ctccttggca gaaaggaagt tcagttcgat tgggccaagt tcacctggat
     2701 cggcacgccg gaagaaaacg acgagctgtt gtttgttcgc tccgatcagc cgtacaaaaa
     2761 cctgttagat ttgcgcaagg ccaccgaacc gctgcgctgc ggcgcttcgg gagtcggcag
     2821 cacgggattc tacataccca agttaatcga ggagatcttc ggcgccagga tcactgtggt
     2881 gaccggctat ccgggagctg cggacgtgga tttagccgta gagaaaaacg aggtccactg
     2941 ccgggggacg accatcagcg ctttcttcgg gcgcgagccc ggaagaacct gggccaagaa
     3001 cggctttgtc agattcctgg tgcaaaccgg cgatcggcgc aaccaccgct tgcccgacac
     3061 gccgacgatg tgggagttga tggatcagga aaagggcacg gatgcgacga aacggctggc
     3121 gcgagtcatt cttggcccag gcgctttcgg ccggccgatc ctggcgaccc cgggcatccc
     3181 tgctgatcgt gtaaagctgc tccgcgaagc ctatgtcaac atgctgaaag atcctgaatt
     3241 tctcgccgaa gcaaagaagc gtcaatggga gatcgcaccg gtcagcggcg aacggctgga
     3301 ggctttggcc aaagaagtcg tcaatcagcc gccggagatc atcgagcgga tgaaaaaagt
     3361 cctgggcgaa tcgtgacagc cagcgtactg gaggcgaaag atggacttag caaaatacat
     3421 cgtcaaagct cggcccggtg tgctcggtgg caagcatgag gagaagcggc cgctccgtca
     3481 attgagcgca ctgccggtaa agcgttatgt gttcatcaat cgcgattcgc accctgatgc
     3541 cgatatttac gtggcgatcc accaagccaa ggatctgcct tcgccggttc ccgactatca
     3601 ggtgccgcat tgtcacaata ccgacgagtt ctattacttc atcggtaatc attcggatct
     3661 cacggggctc gaaggtcaga tcatctttga agggaaagtg cacaagatcg tttcacccgc
     3721 gtgtgtctat attcccacgg ggacagtgca cgagtacaag gtgactgccg gcaacggcac
     3781 tgtgacggtc ttgtttcgca accgcggtta cactcatgag gataagccgt tcgatgtcgg
     3841 taaaggcgaa cgcgactttg cacgatacgc cggttacatt tttaagccgg aagtgcggcc
     3901 gacgacggag atcaagtatc ataccgaggc ggcgccgggg acgcgttatg tttttgtcga
     3961 cgggaagttg aagccggagg ccaacttcta caccgtggta cgcagcgtcg ccggggtgct
     4021 accgacccaa gcgaactatg tcgatatgca ccggcacaac tgcgacacgc agcacattgc
     4081 gatcggcacc ggacctgaat tgaccggact caaaatcgag ttccagatcc gcgatgataa
     4141 aatggtcgtc gagagcccgg tcggagtgaa cattcccgcg ggcacgccac actcgcagcg
     4201 catcatcgag ggcgcgggcc atttcttcaa ctttgtgccg aaaagctttt acaatgacgg
     4261 gctgatgagc tgaaatattg cgtcggtgat ttccccggca cgatcacatg gtataaagca
     4321 ccttatcgac tctcggcttg cgcgccattt cggcttctat ctctaggcac cctcgataga
     4381 tcaataatac atgcctggca ccaaatcccc ccgacgcgag tgtcatgccg taaggattga
     4441 cgtaacgatg aattcccaat aaggatttag cagacctttg cttggtgcgg gcaatttaat
     4501 cggaggagtt cccatggtca ggtttcaatc cagttccgtc gtcttcacag tccttgtggc
     4561 actgttgctc gccagcctcg cagcgtcccc ggccctttcg caagcgccgt tctaccaggg
     4621 caagacgatc acactgatcg tcggcgcagg tcctggcggc atgggcgatc tgcgcgccaa
     4681 agcgctcgcg tcggtactcg ccaagaacat tcccggcaat ccctccatca catttcaata
     4741 catgccgggg gcgggggggc gaaaagcggc aaatcacctc tacaacacgg cgcgtcccga
     4801 cggccttact ctgtaccgca tctcgagcag catcgtttct tacggggtgc ttggcgaaac
     4861 cggcgtgcag tacgatgtcg acaaactcat ctacctcggc acgaccgagc atcagctcta
     4921 ttatgtcttc atgacgcgcg cggcgctagg ccacgacagc ctgacaaagc tgcgcgcggc
     4981 cgccggtttg cgcatcggcg gcgctccggt ggggcatacg gggtacctgc acagccgaat
     5041 gatcgcctat tttctcgacc tagggaattc acggatcatt ccgggttatg aaaatgaaga
     5101 ccttgatgtc gccttcgcgc gcggcgaggt cgatgcccag gtcgcgtcca tgggcacgat
     5161 tagccagcac gacttcctga aaaacaaggc cgtgcatttt cacgcgggca tcgaatatcc
     5221 acggggctgg aaagacccgc gcttttccca tctcaatctg cccgatctcg aaagctttgc
     5281 gaaaacggaa agcgaaaaaa aacttctggc tatgatgcga aacttgcgat ccgtgggtac
     5341 ggcattgctg ttccccccgg gcgtgccgaa ggatcgggtg gatattctga aggaagccgt
     5401 gcgcaagacc cacacagatc cggcgttcat cgccgagtac cgcagactca ccagcggcga
     5461 cattccgacg ccgctgatgc ccgacgagca ggcgaaagtt gtacgggaga ttccgcgcga
     5521 cgcggaaacg atcgagctgt tcaaaaaatt cgctggaaca agcgcattgc cgccgcgcca
     5581 gtaggaggtg ccttgcgttg atagcagggt gctgaaaaga gcctcttgca ggctgactgc
     5641 gcctttcaat cgaaaagtag cctgtcctct ttagctcctc taatattgaa ctgatcttgg
     5701 aaagcccacc gcgccggttt cgatcactat atgaactgat tggacatcaa cacgtaggcg
     5761 tacacgatgc agcgatagcg ctctcgctaa tgctcgatcc gatcaaggta gccacgccgt
     5821 cgctttggtc gcgaaagata tcgcgtcggt gatttcccgg caggatcaca taatataaag
     5881 caccgtcgaa ctcgactctc ggcttgcgcg cctatct
//
LOCUS       DASUTG010000230        13461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1816555, whole genome shotgun sequence.
ACCESSION   DASUTG010000230 DASUTG010000000
VERSION     DASUTG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13461
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816555"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VF089_05130"
     CDS             <1..829
                     /locus_tag="VF089_05130"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX6173376.1"
                     /translation="CHTPHAIRPPNDSRSSVHPTRVADTCGSCHADADYMAPYKIPTD
                     QLKQYQQSVHWRMIFNKSDLSAPTCNDCHGNHGAAPPGVSSVANVCGQCHAVNAELFN
                     KSAHSKIFAQMGLSGCATCHSNHGIMETGDVMLAAGEKSACATCHPPGSSGGALAASM
                     LQSIDRLKGGYEKALAVLKDAEHAGMEVSQPLFELNEAKTTLIKARATIHAFDQTLLD
                     KEVEPGLAVSEKAYARGLKAMNELQFRRKGLAISTLIILALIIGLILKIRQMERKPK"
     gene            850..1308
                     /locus_tag="VF089_05135"
     CDS             850..1308
                     /locus_tag="VF089_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6173377.1"
                     /translation="MADERSIALDNGRRRFLNWFLGTTAGAFFISVTYPLSRYLIPPA
                     VEESTAHTVTLALKPQEVKPNSGQIFRFGSQAAILIRTPNDELKAFAAACTHLACIVQ
                     YRSDISHIWCACHNGHFDLNGKNIEGPPPKPLEEYIVNVRGDQIVVNKRA"
     gene            1321..2412
                     /locus_tag="VF089_05140"
     CDS             1321..2412
                     /locus_tag="VF089_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX6173378.1"
                     /translation="MPTPKHGKLWLWLDERIGLSDFAYLAKKKEIPLHRHTFWYYFGG
                     MTLFLFMIQVISGILLLLYYRPSAGEAFESIQFLMTEVEFGWLVRSIHSWSANLMIFA
                     MFIHLFSALFLRAYRSPRELTWLSGAGLLVLTLAFGFTGYLLPWNELAYFATRVGTEI
                     PGVVPVVGPFLRRLLRGGDDVTGATLTRFYGIHVAILPAIVTVILGLHLFLVQKHGMS
                     RPGSGETGEATKRTMPFFPNFFLRDLVGWLCALGVLAALAAYFPAELGKKADPFQPAP
                     AGIKPEWYFMAMFQTLKLLPAHILGIEGELLGVLGFGLVGLVLICMPFLDRRAALGKR
                     SRWPAAFGIALVVYSLVVSYLGYTMSPTK"
     gene            2646..2975
                     /locus_tag="VF089_05145"
     CDS             2646..2975
                     /locus_tag="VF089_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6173379.1"
                     /translation="MGHKISITIAGLLVWAGTTSAAIAGDADSGKALYEKSCAGCHGA
                     DGKGNEKMAKLLGDKGLNIVGAETKKKSDEQLLKLIAEGSGKMPASKLNKEEQKQALG
                     YARSLAK"
     gene            complement(3054..3245)
                     /locus_tag="VF089_05150"
     CDS             complement(3054..3245)
                     /locus_tag="VF089_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173380.1"
                     /translation="MTENESRLHRKLKIAAYLLLAGLAVEGITLNWSHPTSFLLFIIL
                     GGVLILAGIAVYLVEIVTG"
     gene            complement(3242..3850)
                     /locus_tag="VF089_05155"
     CDS             complement(3242..3850)
                     /locus_tag="VF089_05155"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX6173381.1"
                     /translation="MANYAVATVILLSIILIGVLLTRPSITAAPGGKIVAFIAFFILP
                     IVATVLGASIHLEQSTSTEFCLSCHVMEPYGKSLLIDDPSYLPAAHFQNNRIPKDHAC
                     FTCHTDYTMFGDVNSKMRGLKHIYVYYLKTPPAKLALYEPFKNRECLYCHANGRSFEE
                     TSPHTEMREALASNDMSCLTCHMKVHDVANVEKLKKWKGPGV"
     gene            4127..4456
                     /locus_tag="VF089_05160"
     CDS             4127..4456
                     /locus_tag="VF089_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6173382.1"
                     /translation="MKFIVLTIAVILTSAWPTTQGLAADAETGKTAYEKNCAGCHGAD
                     GKGNPAVAKTLGEKGLNIVGKETTEKSDEVLLKIIAEGSGKMPPSKKLTKEEQKAVLQ
                     YSRSLAK"
     gene            complement(4885..6348)
                     /locus_tag="VF089_05165"
     CDS             complement(4885..6348)
                     /locus_tag="VF089_05165"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX6173383.1"
                     /translation="MSIEQIFIAAGIVFLLSVFASKVSENYGIPALLLFLVLGMLVGS
                     EGPGGIYFDNASLAQALGVTALALILFSGGLDTSWSEVRPISFIALVLSTVGVLITAG
                     VIGLLAWFLLGFNLLKGLLLGSIISSTDAAAVFSILRSRNIGLPPQVKSLLELESGSN
                     DPMAVFLTVALIGFLGQEASAFTLLIQMFLRQMVLGAVLGFALGWALVWLLNHIGLET
                     EGLYPVLTLSCVLLIYGVIAYLGGNGFLGTYVAGLVMGNKSFVHKRSLLRFHDAVAWL
                     MQIVMFIILGLLVFPSQLVPVIGSGLLLSGLLMLVARPLAIFLCLLRSRFNLKERLLI
                     AWVGLRGAVPIILATFPLLANVPDAGHIFHLVFFIVLTSVLLQGTTIPMVVGYLGLAT
                     PQASKLRYALELEEGEGMETTLMDFIVPYNSPVIHKAMVEIRFPEQSVVALISRNNEY
                     IVPTGATRLQGGDVLLMILNKGAVAATHRILSTGKQE"
     gene            complement(6584..7732)
                     /locus_tag="VF089_05170"
     CDS             complement(6584..7732)
                     /locus_tag="VF089_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173384.1"
                     /translation="MHSLINDYQAGLLGAHEPPCLSLYQPTHRAYPENQQDPIRFKNL
                     LKSLEQSLRQKYTTRDVKPLLQPFQDLSANQDFWNHTQDGLAVLGAPGLFRVYRLQRV
                     VPELSVVADSFHIKPLIRILQSSDRYQLLGLNRREVKLFEGNRDALDEIEPAPGVPRT
                     ITDALGDELTEPHLTVASYGTGAAGPAMRHGHGAKKDEVDVDAERFFRAVDRAVLEHH
                     SRPSGLPLILAALPEHHSLFRQVSQNSFLVSEAVDIHPDALSGEALRERAWRVIEPRY
                     LARLATLVDEFGKAKSNGLGSDDAALIAEAAAAGRVATVLIEAERHVPGRIDQVSGRV
                     EFGDLAHPEVDDLLDDIGELVLEKGGQVVVVPAERMPVETGVAAIYRF"
     gene            8155..8868
                     /locus_tag="VF089_05175"
     CDS             8155..8868
                     /locus_tag="VF089_05175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6173385.1"
                     /translation="MAASSTICPPQYLQSPFEVLPALLSSDGSTRLILIRHGHIAANT
                     GGHDTLMAGWTDVPLSSRGRLEIERLKKYLSRGPRFDAIYSSPLSRASETACLLAVAG
                     LGPLRLCPALKEINCGQLDGLPVGEVQGRFPDLWRENCAQEREDFRWPGGESYREFRD
                     RCIAAVRSIAAAHLSGRALIVTHAGVISQIIGFLSGVSPARWGLFRPGNASITELAWR
                     GCSGVVVSFDSRTHLSDAV"
     gene            complement(8877..9911)
                     /locus_tag="VF089_05180"
     CDS             complement(8877..9911)
                     /locus_tag="VF089_05180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6173386.1"
                     /translation="MKNSSTQSNRIYHIIERLHGQAPRGLRLQVKALRGGLEALAVLR
                     VQADFRNSRGRRQTYYMAVKRLDGTRAREASVYDKLVVRYAQTIAPRVLDVDHQGSEQ
                     SILYLEWVRPVTHWPWRELSTAEKFMGQLARLHMSTASDDVAAALPQWNYDAELKRSS
                     ETTLELLARCRRHPEFAPLGRLLAPARHVAEALPELRQQLLAFTPLGRVAIHGDVHPG
                     NVIVRRRGGNEEPILIDWGRARAGSPLEDLSSWLQSLSYWEPRARLRHDTLLGSYLSA
                     RGMERRLSSNLRTAYWLAGASNALSGALEYHLSLLLGQTPATVLQKQAAAQSARDWLR
                     IVRRAAACWH"
     gene            10933..13257
                     /locus_tag="VF089_05185"
     CDS             10933..13257
                     /locus_tag="VF089_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173387.1"
                     /translation="MSTAPGTLFSRLLKDHVRSISDRHLSIRSHLILLVVAALLPVLI
                     FAGIMFWQHVELQRATVDQGMRDTARALSLAVDREIGQVREVLETLAASPYLDSQDFK
                     AFYELSSRAAKEHNNSSIVLLDRSGQQITNTLRPFGVPLPSAVQHAKEPPADDIDQLP
                     QGTPETVQRVIDSGEPVVTDLFMGIVSKRPTVSIAVPVIRDGHVIYVLNMGIMPDKLT
                     ALLVEGRFPSDWYAVIVDRKGLIISRQPDPERFVGRPLTSLRDLVGYVDEGWGAGRTQ
                     EGIDVYRSFVRSKYTGWTVSVGAPQASVDAPVSRSIRILGFGASALAFVAFGIAVILG
                     KRISAPLISLARSAEAIQDGRKVEIEPSSIREVVELHSELIRSVTERQKLEEQLRQAQ
                     KIEAIGTLAGGIAHDFNNTLNIIQAYAMLISKQASGDEHIIESTKVISDEVQRGAAVV
                     RQLMTIARKTEVSLGRTDLNQLILTVSNLIRQTFPKTIELELKLDRSLNPVLADSNQI
                     TQAILNLCLNARDAMPQGGHLTLATELLGPDVSQMRRAELSPKPHVCIAVTDTGSGMD
                     EAVRSRAFEPFFTTKAVGEGTGLGLAMVYGIAKNHKGTVHIESELGAGTTVRLYLPAL
                     FHEYDYSASDLSSRESVQRRKLAEHSTVLVVEDEKAMLQLLGNGLSREGYGVLTAADG
                     AGAIDVYQRYRSDIDVILLDLGLPKVSGLDVIRTIRKLNEDVKIIVTTGYLEPELKSE
                     LTTAGVNEYIQKPYIIPDILGRISHHLSAEELVS"
     gene            complement(13278..>13461)
                     /locus_tag="VF089_05190"
     CDS             complement(13278..>13461)
                     /locus_tag="VF089_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173388.1"
                     /translation="LLKKLVIAVVDPLFGSVAVTNSRSAMLMRTATRDFSVFSKMSHG
                     KTEKRVCVEQDQNAAI"
BASE COUNT         3252 a         3611 c         3641 g         2957 t
ORIGIN      
        1 ctgccatacg ccgcatgcga tcagaccgcc caacgattct cgttcgtcgg tgcacccaac
       61 ccgggttgct gacacctgcg gctcgtgtca cgccgatgcg gattacatgg cgccgtataa
      121 aattccaacg gatcagctca agcagtatca acagagcgtc cactggagga tgattttcaa
      181 caagagtgat ttatcggcgc cgacgtgcaa cgactgccac ggcaaccacg gtgcggcgcc
      241 gcccggagtc tcttccgttg ccaacgtctg cgggcagtgc catgcggtca atgccgagtt
      301 gtttaacaaa agcgcgcata gcaagatctt cgcgcagatg ggcctttccg gctgcgccac
      361 ctgtcacagc aatcacggca ttatggaaac cggcgatgtg atgctggcag ctggcgagaa
      421 gtcggcatgt gccacctgtc atccccccgg ttcttcgggc ggcgcgctcg cggccagcat
      481 gctccagtcc attgatcggc ttaagggggg ttatgaaaaa gctctcgcgg tgctaaagga
      541 tgccgaacat gccggcatgg aagtgagcca gccgttattc gagctcaatg aggccaagac
      601 cacgctcatc aaggcccgcg caacgattca tgcattcgat cagacacttc tcgacaaaga
      661 agttgaaccc gggttggcgg taagcgaaaa agcctatgcg cgaggcctca aagcgatgaa
      721 cgagcttcag ttcaggcgca agggattggc tatttcgacg ctaattattc tcgccttaat
      781 cattggattg attctcaaaa tccgccagat ggaaagaaaa ccaaagtaac cttccatgaa
      841 gaggtaaaag tggccgacga aagatccatc gctctggata acgggcgcag acgttttctc
      901 aattggtttt taggcaccac ggccggagcg ttcttcatct cggtgactta cccgctgagc
      961 aggtatttga taccgcccgc agtggaagag tcgacggcgc acacggttac tttggcgctc
     1021 aagccgcagg aagtgaaacc gaacagcggg caaatcttcc gtttcggcag ccaggcggcg
     1081 atattgatca ggacgcctaa cgacgagctc aaggcttttg cggcagcctg cactcacctg
     1141 gcgtgcatcg tccagtaccg atccgatatc agccatatct ggtgcgcgtg ccacaatggc
     1201 cacttcgatt tgaacggcaa gaatatcgag ggacctccgc ccaagcctct ggaagagtac
     1261 attgtcaatg tccgaggcga tcaaatcgtc gtcaacaaaa gagcttgaaa tcagatggaa
     1321 atgcccacac cgaagcacgg taagctttgg ctctggctcg atgaaaggat cggcctcagc
     1381 gattttgcct atcttgccaa gaaaaaagag ataccgctgc atcgtcacac attttggtac
     1441 tacttcggtg gcatgactct gtttctattc atgatccagg tcatcagcgg cattctgctc
     1501 ttgctttact atcgaccgag cgccggcgag gctttcgagt cgattcaatt tctcatgact
     1561 gaggtcgagt ttggctggct ggtaagatcg attcactcct ggtcggccaa cctgatgata
     1621 tttgcgatgt tcatccacct gttcagtgct ttatttctta gagcgtaccg ttcgccacgc
     1681 gaactaacct ggctttcggg ggccggactt ctagtcctaa cactggcgtt tggttttacc
     1741 ggatatctct tgccttggaa tgagcttgcc tacttcgcga ctcgcgtcgg cactgaaatt
     1801 cccggggtcg taccggtggt tgggcctttt ctgcggaggc tgctgcgagg aggagacgac
     1861 gtgactggcg cgacactcac aaggttctac ggcattcatg tggcgatttt gccggccatt
     1921 gtcaccgtca tcctcgggct tcatctgttt ctggttcaga aacacggcat gagcagaccg
     1981 ggcagcgggg aaactggcga agcgaccaaa aggaccatgc cttttttccc gaactttttc
     2041 ctccgcgatt tggtaggatg gctctgtgcc ctgggggtgc ttgcggctct cgccgcctat
     2101 tttcccgcgg agctgggaaa gaaagccgac ccgtttcagc cggcgccggc tggcatcaag
     2161 ccggaatggt atttcatggc gatgttccag acactcaaac tgctgccggc gcatattctt
     2221 ggcattgagg gtgagcttct cggcgtgctc ggattcggtt tggtgggcct agtgctgatc
     2281 tgtatgccgt tcttggaccg ccgagccgcg ttaggaaagc gaagccgttg gccagccgct
     2341 ttcggtatcg cgctagtcgt ttactcgctg gtggtgagct atttgggata tacgatgagt
     2401 ccgacaaagt gagtctgtgt agtttggcaa acttggcaaa cgccggaatc atgatagcgc
     2461 gcaccgaacc tgaattgttg gccgttaacg gcgtctaatc ataaagccct gtccgtggcg
     2521 ataaaatcga ccagccgcca ggcgccacaa cattatttcg cgcggggagg acacggctgg
     2581 ttggcaaaag cagcgtctga ccagacacga cgattgcaaa acaagcatta aggagaaacc
     2641 gacaaatggg tcataaaatc agcataacga tagctggatt attggtttgg gcggggacga
     2701 cttctgctgc gatcgccggc gacgccgaca gcggcaaagc actttacgaa aaatcctgcg
     2761 ccggctgtca cggtgccgat ggcaaaggca acgagaagat ggcgaagcta ctgggcgata
     2821 agggcctcaa tatcgtcggt gctgaaacca aaaagaagtc tgacgagcaa ttgctcaaat
     2881 tgattgccga aggttctgga aaaatgccgg cttccaaatt gaataaagag gagcaaaagc
     2941 aggctttggg atatgctcga tccctggcga aataggccga agcgaaatct cagcgtagcc
     3001 gctgaagtgg gttcgtcaaa tcagtgcctc gtatggaatg ccatgtccga tgctcagccc
     3061 gtcacgattt caactaagta gacagcgatg cccgctaaaa taagaacgcc gcctagaatg
     3121 atgaaaagca gaaagcttgt cggatgcgac cagttgagag taatcccttc aacggccaga
     3181 ccggcaagca acaagtaagc tgctatcttc agttttcgat gtaagcgcga ctcgttctct
     3241 gtcatactcc gggccctttc cactttttta gcttttcgac gtttgcgaca tcgtggacct
     3301 tcatgtggca tgtcaagcag gacatgtcat tgctggcaag cgcttcccgc atttccgtgt
     3361 gcggcgaggt ttcttcaaag gatcgcccgt tggcatggca gtataaacat tcgcggtttt
     3421 tgaacggttc ataaagcgca agtttcgccg gcggagtttt taagtaatag acgtagatat
     3481 gcttgaggcc gcgcattttg ctgttcacgt ccccgaacat cgtgtaatcg gtgtgacagg
     3541 taaagcaggc atgatctttg ggaatacggt tgttttgaaa gtgggcggcg ggaagatagc
     3601 tgggatcatc gatcaaaaga cttttcccgt agggctccat cacatggcaa gacaaacaaa
     3661 attccgtcga ggtagattgc tccaaatgaa ttgaagcgcc cagaaccgtc gctacaatgg
     3721 ggagaataaa gaaggctatg aacgcaacaa tcttgcctcc cggcgccgca gtaatagacg
     3781 gtcgcgtcag caatactccg atcagaatga tactcagaag gatgacggta gcgactgcat
     3841 agtttgccaa aattaattcc ttccgggtcg gtgtcgcgca ccggttaatg actctcgata
     3901 ttcacctcgt ataccgaatg gttgtcgaat tatccagtaa aacttttgca cgagctattc
     3961 ggccattacc agtcgggatc gataatctat gcccagctga gattcctcgg aaggtgatat
     4021 ttagatagcc ggcgtgcctg gccgctcaaa agccgggaga ttgttgacag tcacttgcct
     4081 agattgtact cctcaagaac gacaaaaagc caattaggag gactccgtga aatttatcgt
     4141 actgaccatc gccgtaatcc tcacttcagc atggccgacg acgcagggac tggcagcgga
     4201 tgccgagaca ggcaagacag cctacgagaa gaactgcgcc ggttgtcatg gggcggacgg
     4261 aaaaggaaac ccggcggtgg ccaaaacatt gggcgaaaaa gggcttaaca tcgtcggtaa
     4321 agaaacgaca gaaaaatcgg acgaagtatt actaaagatt atcgctgaag gttcgggcaa
     4381 gatgccgccc agtaaaaagc ttactaagga agagcaaaag gcagtcctgc agtacagccg
     4441 atcgttggct aaatagcccc gtcgccggga caaggggatg gccgacaatt tacagcggct
     4501 atgatcggta tctccggcat ggccgcacgg tttgagacgt caagtctgcc ccacagccaa
     4561 actcccacca acgtaagatt ggagccgaac ctggatgtcc gaacttactc caaacggcct
     4621 gcgatattga aactgtgatt tgatccatcc ccgatccttt agctcgggtt ctatcctagt
     4681 tccgcggaag ctcagccgtt ggtgtcgcag tcgattgatc atcgcttcga cactgtagaa
     4741 atatcgatag ccgacctcac tcggctctac cacgttgaag ctcactccgg tgtcaataaa
     4801 atagcaagag tagacggctc atcaactgac aaagagaaga tctttgagat taatacgtaa
     4861 gcggcgcccg ggggtgataa gcctctattc ttgcttgccg gttgagagga tccgatgggt
     4921 tgcggcaaca gcgcctttgt tgagaatcat cagcaacaca tctcctccct gtagccgggt
     4981 ggcgcctgtg ggcacgatgt attcattatt tctggagatg agcgcaacca cgctttgttc
     5041 gggaaatcga atttcgacca tggctttatg aatgaccggc gagttgtacg gcacgataaa
     5101 atccatcagg gtcgtttcca tgccttctcc ttcttccaat tccagcgcgt agcgtagctt
     5161 ggaagcttgg ggcgtcgcta gacccaagta acctactacc atcggaatgg ttgtgccctg
     5221 aagcagaacc gaggtaagta cgatgaaaaa caccaagtga aagatatggc ccgcatcggg
     5281 aacattggcg agcagcggaa aagtggccaa aatgatcggt acggcgcctc gaagtcccac
     5341 ccaggcgatc agcagtcgct ccttcaagtt gaaacgactc cgcaacaagc acaagaaaat
     5401 tgccaatggc cgggcaacca gcatcaacag acccgacaga agaagcccgg agccaattac
     5461 tggaacaagc tgcgacggga acaccagcag ccccagaatg ataaacatca cgatctgcat
     5521 cagccaagcc accgcgtcat ggaatctcaa caggcttctc ttgtgcacga agctcttatt
     5581 gcccatcacc aagccggcga cgtaagttcc gagaaacccg tttccaccga ggtaagcgat
     5641 gacaccatag atcaacaaca cacaggaaag cgtcagcact gggtaaagac cttcggtctc
     5701 caggcctatg tggtttagca accagaccag cgcccagccc agcgcgaagc cgagaaccgc
     5761 gcccagcacc atctgccgaa gaaacatctg aatgagcaga gtgaaagcgc tggcctcctg
     5821 acccaagaac ccaatgagag cgactgtcag aaacaccgcc atcggatcgt tgctgccgga
     5881 ttccagctct agcagactct tcacttgtgg aggcaacccg atgttgcgcg agcgaagtat
     5941 cgaaaacaca gcggccgcgt cggtggacga gatgatcgaa cccagaagca gtcctttgag
     6001 caaattaaag cccaatagaa accaggcgag taacccgatg acgccagcgg taatgagaac
     6061 tccgacagta gaaagcacca gggcgatgaa gcttatcggg cgaacctcag accagctggt
     6121 atccaggcct cccgaaaaga gaatcaaagc aagcgccgtc acgcccagcg cttgagcaag
     6181 ggatgcgtta tcgaaataga ttccccccgg cccttccgag ccgacgagca tccccaaaac
     6241 caaaaacaat aacaacgccg ggatgccgta gttttccgat accttgctgg caaaaacgct
     6301 caggagaaag acaatgcctg cggcgataaa tatctgttct atggaaatca ctaaagtgtc
     6361 tcgaatgttt agttattcat cggcggcgat gtcatgaata tctaaatcgc gcgcacggct
     6421 tccatttata aacagtatct ccttgtggct aaattgcctc tctggccccg gcgcacagcc
     6481 ggacaattaa gatggtagtc ctctgccctg attggttggc ctatggcgat cgtgtctcgc
     6541 gaccggctat cgcaaggagc attcgttttg gcgcgattaa ctcctaaaag cgatagatcg
     6601 ctgcgacccc ggtttctacc ggcatgcgct cggctggaac gactacgact tgcccgccct
     6661 tttctaggac cagctcgcca atatcgtcga gcagatcgtc gacctccggg tgcgcgaggt
     6721 cgccaaattc aacgcgccct gacacttgat cgattcgccc gggtacgtga cgctcagctt
     6781 caatcaatac cgtggcaacc cgtccagccg ccgcggcttc agctatgagt gcggcgtcgt
     6841 cagagccgag tccgttcgac tttgctttgc cgaattcgtc gaccaacgta gcaagccgcg
     6901 caagatatcg gggctcgatg acccgccagg cgcgctctcg gagcgcctca cccgatagcg
     6961 catcaggatg aatatcgact gcttctgaca ctagaaatga attctggctc acctggcgaa
     7021 acaagctgtg atgttctggc aacgccgcta aaatgagcgg tagcccagag ggccgcgaat
     7081 ggtgctccag aaccgctcgg tcaacagcgc ggaagaaccg ttctgcgtcc acatccacct
     7141 cgtccttctt tgcgccatgg ccatggcgca tcgcggggcc tgcggcaccc gtgccgtagg
     7201 aagccacagt caggtgtggc tcagtcaact cgtccccaag cgcatcagtg atcgtgcgcg
     7261 gcactccagg tgccggctcg atttcgtcga gagcatcgcg attcccctcg aagagcttta
     7321 cctcacgccg gttcaatcct aataattgat agcgatccga cgactgaagg attcgaatca
     7381 gcggcttgat atggaaacta tcggccacca cactcaattc aggaacgacc cgctggagcc
     7441 ggtaaacacg gaacagtccg ggtgcgccca agaccgccaa gccgtcttgc gtgtgattcc
     7501 aaaaatcctg gttcgccgaa agatcctgaa acggttgcaa caggggctta acgtctcgcg
     7561 tggtatactt ctgacgcagc gactgctcca gggacttaag taaattttta aagcggatcg
     7621 ggtcctgctg attttcagga tacgccctgt gcgtcggctg atagagtgat agacagggcg
     7681 gttcatgagc gcccaatagc ccagcctggt agtcattgat caacgagtgc atcgttcctc
     7741 ctattttatc taagtttgta tcagtgcact tactctcgaa tcagagaatc ggcctagact
     7801 ggctaggctc tgaggagcct tctgggtatc gagaaggagt gagtgcccgg cgcgccgagc
     7861 cttcagagtc cgcgcgacga tgcgccagga ttcctccgcc ttctcgagcc cgtgaccgcc
     7921 gaatctctac ttacgaattt tctggttcat catctaatgg ttctgtctaa cgtgaaattc
     7981 taggttcacg caatcgcgct gctatcctaa agtcgagtat cggtcggcag gagcagtagt
     8041 aacctgtctc taacaaggga cggcgcgaag gtggttgatc gcgcagagat tctgaacgta
     8101 tactcaactt aggataggag acatcacacc tagagattga agtgatgtag aaccgtggcc
     8161 gcttcgagta ccatctgtcc accgcagtat ttgcaatctc cgtttgaggt gctgcctgca
     8221 ttactgtcca gtgatggttc gactcgtctg attctaatcc ggcacggaca catcgcagct
     8281 aataccggcg gtcatgacac gctcatggcc ggatggaccg acgttccgct cagctcacgg
     8341 gggcgtctgg aaattgagcg actcaaaaaa tatctgtcac gcggcccacg cttcgacgcc
     8401 atttattcaa gtccgctttc gcgcgcgagt gagaccgcgt gccttctggc cgtcgccgga
     8461 ctagggccgc ttcgcttgtg tccggcgctc aaagaaatca actgcggtca gctcgatggg
     8521 cttcctgtgg gcgaggtgca gggcaggttt ccggatttat ggagggagaa ctgcgctcaa
     8581 gagcgtgagg acttccgctg gccaggaggg gagagttacc gagaatttcg cgatcgctgc
     8641 atcgccgcag tgcgcagcat cgccgcggca cacctgtccg gccgggccct gatcgttacc
     8701 cacgccgggg tgatcagtca aattatcggc ttcttgtcgg gcgtgagccc agctcgatgg
     8761 gggctgttcc gcccgggcaa cgccagcatc acggagctcg cctggcgcgg ctgcagtggc
     8821 gtggtagtct ctttcgatag ccggacgcat ctcagcgatg ctgtatagtc gttccgtcag
     8881 tgccagcacg ccgccgcgcg gcggacgatg cgcagccagt cgcgcgccga ctgggctgct
     8941 gcctgctttt gtaaaaccgt agccggggtc tggccaagca gcaacgagag atgatattca
     9001 agagcaccgg agagcgcatt tgaagctccg gcaagccagt aagcagtcct taggttggat
     9061 gagagacggc gctccattcc tcgagcggag aggtaactcc cgagcagcgt atcgtgccgc
     9121 agtcgtgctc gcggttccca gtagctaagc gattgcagcc aactgctgag atcttccaga
     9181 ggtgagccag cgcgggcgcg tccccagtct atgagaatag gctcttcatt gccgccacgc
     9241 cgtcgcacga ttacattgcc gggatggacg tctccgtgaa tggccacgcg cccgagcgga
     9301 gtaaatgcaa gcagttgctg ccgcagctcc ggcaaggctt ccgcgacatg ccgggctggc
     9361 gcaaggaggc ggcctaaagg cgcgaactca ggatggcgcc ggcagcgtgc aagcaactcc
     9421 aacgtagtct cgctcgatct ttttaactca gcgtcataat tccattgcgg cagcgcggca
     9481 gcgacgtcat ccgacgcggt agacatgtgc aatctagcta gctggcccat gaacttctcg
     9541 gcagtggata actcccgcca tggccaatgg gtgaccgggc gaacccattc aaggtacagg
     9601 atcgattgct cgctgccctg atggtccacg tcaagcacgc gcggcgcgat cgtctgcgcg
     9661 tacctgacta ctaatttgtc gtaaactgag gcctcgcgtg ccctggtacc gtccagccgc
     9721 ttgaccgcca tgtagtacgt ttgcctgcgc ccgcggctgt tccgaaaatc cgcttggact
     9781 cggagcaccg ccaacgcctc cagcccgccg cgcagcgctt ttacttgcag gcgtagccca
     9841 cgcggagcct gcccgtgcag ccgctcaata atgtggtaga tgcgattaga ttgtgtactg
     9901 gaatttttca ccactagaga atgacaaaat cacaggcgca ttcaacgtag ctggccagtt
     9961 ctgctgcaat ggcgctcgat cggagtgatg gaagctcagg agttaattta tcggcacgtg
    10021 aaaggttggc gtagctttcg actataacca cgatatgtgc tgtggtgccg ccaagccgga
    10081 actgtcggga cggtgacgtc tcgagccaat tgctgggaaa agatgccgtc cataaccacg
    10141 ttgaacatca gctagcatgc ttccgctgga gagcgtgtga aagttgcgta ggaagactcc
    10201 ttccaacttt atgcggaccg tcacgcgaga gaccgcggcg cttgtgacca gaccacatag
    10261 aatttcttcg gcggttttaa attctccgaa ggagccaagt gttgaatttg cagatcgatg
    10321 atcgagtttt tatttgatcg cgagtaatgt tggcctcacc aacggccgcc ggaacatctg
    10381 gcgcatttcg cagcactact ctctgcgcgc accccaacat gagaagcgcc actcctaaca
    10441 gcaatccgga attcaagaac cttccaaagg ttttcatcac tctctcctgt gcagaacatt
    10501 tcgccgtgca tgtcaacttt aaagttttca acgacgccat gtccgagcaa agtataccga
    10561 atggccgaat gatgggcgag atctgatcct gccgagtagt ccgtaaccgc gaagtttaga
    10621 gttaaagttc ttaaaacgag tgcagcagaa ggtctgctga agtggaacca agttacgtat
    10681 gtcccatttc ggaaagggtt cagcgcggca ccgtcctaat ttcggactgc cgccggagta
    10741 acacactttg acagtgtata acccgggtgt tctcttacac tacggcgcca atcgaccgga
    10801 tcgaatgctg tcttgctctt gtgtcctgag acaacaggcg gcgggttgac acctgattcg
    10861 ccgaattgta gcgtgctccg atcccacaaa gtttaactgt cgtctcgcgt caaagaactc
    10921 atcggaggtt cagtgtccac agcgccaggg actttattct ctcgactgct caaagatcac
    10981 gtccggtcga ttagtgaccg gcatctttct attcgctcgc atctgatcct gcttgtagtt
    11041 gcggcattgc tgccagtgct tatctttgcc ggaatcatgt tttggcagca cgttgagctt
    11101 caacgcgcca ccgtggatca ggggatgagg gatacggcgc gcgctctgtc acttgcggtt
    11161 gaccgtgaaa taggacaggt gcgtgaagta ctcgaaacgc ttgcagcctc gccgtacttg
    11221 gattcgcaag actttaaagc cttttatgag cttagttcac gagccgccaa agaacataac
    11281 aactcatcca tcgttctcct tgatcgttcg gggcaacaaa tcacgaatac attgcgacca
    11341 tttggagtcc cactgccaag tgcagtacaa catgcaaaag aaccccccgc cgacgacata
    11401 gaccagttgc ctcagggcac accagagacg gtgcaaagag tgatcgacag cggcgaaccc
    11461 gttgtaacag atctcttcat gggaatcgta agcaagcggc cgaccgtttc gatcgctgtt
    11521 cctgtaatac gagatggcca cgttatctac gtgctcaata tggggatcat gccagataag
    11581 ctaaccgcat tgttggtcga agggagattc ccatccgact ggtatgcggt cattgtcgat
    11641 aggaagggtt tgatcatttc gcgccaacct gatccggaaa ggttcgtggg ccggccgctg
    11701 acgagtctaa gagatctggt cggctacgtt gacgaaggat ggggtgccgg tcgaactcaa
    11761 gaggggattg atgtctaccg atcgtttgtc cggtccaaat atactggatg gacggtctca
    11821 gtgggggctc cgcaggcgtc agtcgacgca cctgtgagtc gatctattcg catactcggt
    11881 ttcggcgctt cagcacttgc ctttgttgct ttcggaatcg ccgtgattct tggaaagcgc
    11941 atttcggcac cactgataag ccttgcgaga tcggctgaag cgattcaaga tgggagaaag
    12001 gtcgaaatag aaccctctag cattcgggaa gtagtcgagc ttcattcaga acttatccgc
    12061 agcgtaaccg agcggcaaaa actagaagaa cagctccgtc aagctcagaa gattgaagct
    12121 atcggtacat tggcgggtgg catcgcacat gacttcaaca acaccctaaa tatcatccag
    12181 gcctacgcga tgcttatcag caaacaagcg tctggcgacg agcacatcat cgagagcacc
    12241 aaagttattt cagatgaggt tcagcggggc gcggcagtag tacgccagct gatgacgatt
    12301 gcccgtaaaa cggaagtctc gttaggccgc accgatctca atcagctaat tcttacggtg
    12361 agcaatctta taaggcagac cttccccaaa actattgagt tggaattaaa gctcgatcga
    12421 tcactgaatc ccgtgctggc agattcgaat cagatcactc aagccatcct caacctctgc
    12481 ctcaatgctc gtgatgcaat gcctcaagga ggccatctta cattggctac ggaattgctc
    12541 ggcccggacg tttcccaaat gagacgcgcg gaactctcgc caaagccaca cgtctgtatt
    12601 gccgttacgg atacagggtc cggcatggac gaagccgtgc ggtcgagagc atttgaacca
    12661 tttttcacaa caaaagcggt cggtgaaggg acagggttag ggcttgcgat ggtttacggt
    12721 atagcgaaaa accataaagg tacggtccac attgaaagcg agctaggagc cggaacaaca
    12781 gttcggttgt atctacctgc tttattccac gaatatgact actcggcctc tgacctaagc
    12841 agtagggaaa gcgtgcaacg ccgaaaattg gccgagcact ccaccgtcct cgtggtggaa
    12901 gacgagaaag caatgcttca gcttctggga aacggcttgt cgcgggaagg ctacggcgtt
    12961 ttaaccgcag cagatggcgc cggtgcaatc gacgtttacc agcggtaccg aagcgatatt
    13021 gacgtcatcc tactagatct tgggttgccc aaagtttcgg gtctagacgt gattaggacg
    13081 ataagaaaac taaatgaaga tgtgaagatt attgttacga ctggatatct agaaccagaa
    13141 ctgaaatcag aactcaccac agccggagtt aatgaataca ttcaaaagcc ttacatcata
    13201 cccgatattc ttggacgtat atcgcatcat ttaagcgctg aagaactcgt ctcctgaacc
    13261 aaccattgac taagagatta gatggcggcg ttttgatcct gctcaacgca aactctcttt
    13321 tcagtttttc cgtgtgacat tttactgaac accgagaaat cccgcgttgc agtgcgcatc
    13381 aacatggcgg atcgggagtt ggtcacggca acgctgccaa aaagtggatc gacgacagca
    13441 atcacgagct tcttcagaag g
//
LOCUS       DASUTG010000231         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_926909, whole genome shotgun sequence.
ACCESSION   DASUTG010000231 DASUTG010000000
VERSION     DASUTG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926909"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1260
                     /locus_tag="VF089_05195"
     CDS             436..1260
                     /locus_tag="VF089_05195"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX6173389.1"
                     /translation="MATMITNECINCGACEPECPNNAISQGETIFVIDPQLCTECVGF
                     HDFEACAAVCPVDCCVTDPNNIETEEALIARARALHPEAEIGDNFESRFRKGQGKSGG
                     TTLQKPAPAAAPAESSAAAPSVATAIAPQTTNYAEPADNFVSLPPIDAWEIPLRCFKC
                     GQTHPEPVRNFMIGNVIFCPHCHKSMVVRDNLNYHIRTLLTDSYEKWQKEQEAYSSRR
                     EIELAEFMEKRAKDAQAFESHQRRQLDQIREQLAALGENYDAPGKPIKKGARFGWG"
     gene            1282..1755
                     /locus_tag="VF089_05200"
     CDS             1282..1755
                     /locus_tag="VF089_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173390.1"
                     /translation="MFNSWLHIIALVAYLGAILGLAIMLLPSLASVEKHEDKVQFLAR
                     GLKFYNPLQVGALGLVLFSGAFELTHLKAIYREMFLKELGYNLAIKLTFAFLLVFFSV
                     YQAMGIGHRFVKRRELGESITPTELDSVVRRLSGANWLILFLAIITIWLGLRLQS"
     gene            complement(2038..>2783)
                     /locus_tag="VF089_05205"
     CDS             complement(2038..>2783)
                     /locus_tag="VF089_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6173391.1"
                     /translation="VNAILSVGALGSLLVAQGCIATRDWVKEQMDPVSNRVSQSESRL
                     TEGEGRLTQSEKQIGTMGNRITGIEGKLGQFEGRLGQVDAKADKALNALSNLRLERRF
                     VIDMKEGANFAFGSSNLPNQAQKEIDGFLSDLKGDPSGAGNTVFVVAGHTDNVGTDDI
                     NYELGKRRADAVSRYLITQKKMDPLKVMSVSYGESAPLTENNNAQNRAKNRRVEILVY
                     REAITSEAVAATPQANSPAPQSETLSKRQ"
BASE COUNT          641 a          773 c          706 g          663 t
ORIGIN      
        1 caggctacgt cggcattcac cctcgcgttc tggatacgat ggtgaggcac ataacacgat
       61 catcgaattg acaaacagct gggagactta tcagtatgat cttggcaata ccttggccat
      121 ctggcgctgg gagttgaagc cacttataga acccgcaacg agctacgcaa caaaggcgcg
      181 aaagtcgttc gtgaatcgga tccccatgaa gcatggaaga acggaaatcg cgttcatcga
      241 agatcccatc cgatgtagaa tcgagctggt tgacttgagc cgcgcgggca agcctaaacg
      301 ccgatgctct aagcaacgtg cgtccgagac tagattcgcg gaggtcagca aagattgcaa
      361 ccggatcaga tttaatttac gatcctttgg ttttcacgcc aatacaatcc cgctacattc
      421 aggaggaccg atgtcatggc tacgatgatt actaatgagt gcatcaactg cggcgcatgc
      481 gagcccgagt gccccaacaa tgcgatttcg cagggtgaaa ccatctttgt catcgatcct
      541 cagctttgca ctgaatgcgt cggcttccat gatttcgagg cctgcgccgc cgtttgcccg
      601 gtagactgct gcgtaaccga cccgaataac atcgaaacgg aagaagcgct gattgcacga
      661 gctcgcgcgc ttcatcctga ggcagagatc ggcgacaatt tcgaatcgcg cttccgcaaa
      721 ggccaaggga aatcaggcgg taccacttta cagaaaccag cccccgcagc agctccggcg
      781 gagtcgagcg ccgctgcacc atccgttgcg acggcaatcg cgccgcagac aacgaattac
      841 gccgaaccgg ctgacaattt cgtgtcttta ccaccgatag atgcttggga gattccgctt
      901 cgttgcttta aatgcggcca aacccatcca gagcccgtac gcaatttcat gatcggcaac
      961 gtcatcttct gccctcattg ccacaaatca atggtcgtgc gggacaatct caattatcac
     1021 atccgaacgc tgctaacgga ttcttatgag aagtggcaaa aggaacagga ggcctactct
     1081 agccgtcgag aaatcgagct ggcggagttt atggaaaagc gcgccaaaga cgcgcaagcc
     1141 ttcgaatcac atcagcggcg acagctcgat caaattcgcg agcagttagc cgcgctaggc
     1201 gaaaattatg atgcgcccgg aaaaccgatc aagaaaggcg cccggtttgg ttggggatga
     1261 ttcccgcctg ctttaactct gatgttcaat tcatggcttc acattatcgc tttagttgct
     1321 taccttggcg ccattctcgg tctcgctatc atgctgttgc cgtcgttggc ctcagttgag
     1381 aaacacgagg acaaagtcca gttccttgct cggggcttaa agttctacaa tcctttacaa
     1441 gtaggcgcgc taggtctcgt gctattcagc ggcgcattcg agttgaccca tctaaaagcc
     1501 atctatcgtg agatgttttt gaaggagctg ggctacaatc tcgcaatcaa gctcactttt
     1561 gctttcctgc tggtcttctt tagcgtctat caagccatgg gcattggcca ccgcttcgtc
     1621 aaacggcgcg agctcggcga aagcatcacg ccgactgagc tcgattcggt cgtccgacgc
     1681 ctaagcgggg caaactggct catcttattt cttgccatca ttaccatttg gctagggctg
     1741 cggctccaat cctgaggaac acacgcggcg cgtcttgatc gtcgccagat cggctacccg
     1801 gctctggcca aggaagtacc tcgagcagaa agcaattact cgagccgggc ttgaatatga
     1861 attggaagga agttgcgaga ctttaatatt cccgaagagc ttggctgatg tgcgcagatt
     1921 ttgaacccct ggccggaacg catccacccg ggcttaatga ttgtcgagtc ggctaaggcc
     1981 ggcaattaaa aaggcggggt attctctacc ccgccttttt acccgcactg atacttctta
     2041 ctgccttttg cttagcgtct cggattgcgg tgccggtgaa ttcgcctgag gcgtcgccgc
     2101 aaccgcttcg gaagtgatag cctcacgata gaccaagatt tcgactcgac ggttcttggc
     2161 ccggttttgt gcgttattgt tttcggtcag cggagcgctt tcgccataag aaaccgacat
     2221 aactttgagc ggatccattt tcttttgagt gatcagataa cggctaaccg catcggctcg
     2281 tctttttccg agttcatagt tgatgtcgtc ggttccgacg ttgtccgtat ggccagcgac
     2341 aacaaaaacc gtgttaccag cgcctgacgg atccccctta agatcactga ggaagccgtc
     2401 gatctctttt tgggcttgat tgggcaagtt cgatgaacca aaagcgaagt tagcgccttc
     2461 cttcatgtcg atcacaaagc gtcgttcaag tctcaggttt gagagagcgt tcaatgcctt
     2521 atctgccttg gcatcgacct ggcccagtct gccctcgaat tggccgagtt tgccctcgat
     2581 gcccgtgatc cggttgccca tcgtgccgat ctgcttctcg ctctgagtta gccgcccttc
     2641 gccctcagtc aaacggctct cgctctgaga tacccggttg gataccgggt ccatttgttc
     2701 cttaacccag tcacgcgtcg cgatacaccc ctgagctacg agtaaagaac ctagcgcacc
     2761 gacactcaaa atcgcattaa ccc
//
LOCUS       DASUTG010000232         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2112947, whole genome shotgun sequence.
ACCESSION   DASUTG010000232 DASUTG010000000
VERSION     DASUTG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112947"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..559
                     /locus_tag="VF089_05210"
     CDS             203..559
                     /locus_tag="VF089_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173392.1"
                     /translation="MDQPINGGVGKPVIQGGSKRRYNALGSIALGNRETCDREIAVNR
                     RAVDRAAIRIVALGLRVMIMAVRRSRVVSVVRYKPDSPAALPCCGSTREQHQKRTRDE
                     NQTFHSGRQKILPYGR"
     gene            1561..3024
                     /locus_tag="VF089_05215"
     CDS             1561..3024
                     /locus_tag="VF089_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX6173393.1"
                     /translation="MGKRGVARPMQEMPAHGQAMEMSHEDRLEMLKMHHQQTLWIYWT
                     LVLLGFWVLLSPFTFGYDKAVVEPSGGRQVWLSLEARIQAMVWSDIISGLALIFFGWR
                     SLTVNRPISLWICCFIGIWLNMAPLLFWSPSAAAYLNGTVIGVLVIALTILIPGMPNM
                     IMYMKMGGDQPPGWSYNPSSWAQRWILIVLGFAGWVVSRYLGAHQLGYIDHAWDPFFG
                     ESTYRVLNSRMSHSWPISDAAFGALAYTFEFLMGYMGAPSRWRTMPWMVTIFGILVIP
                     LGLVHIFLVISQPLVVGEWCTLCLLAAAIMLPMIPLEVDEVIAMGQHMLQSKRRGEGL
                     WNVFWRGGSAEGSAEDKRSPELVTLPEKPGTVLKASIWGMTFPWTLALSAGLGIWIMF
                     SPAIFGAGKTSSDLNHLGGALIVVVSVIAMGEPLRLGRYLNSLLGLIIAVAAWFAGDP
                     PTAFQINSVIIGILVALLAVPRGRIVESFGSWDKYVR"
     gene            3014..4792
                     /locus_tag="VF089_05220"
     CDS             3014..4792
                     /locus_tag="VF089_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6173394.1"
                     /translation="MSGEPDIGDYGLIGDCQSAALVSRGGAIDWCCLPDFDSPAIFCR
                     ILDREKGGYWEIQPKAPFTTQRRYLHNSNVLETVFQCETGEARLIDLMPIREDGTGQS
                     LRILRLIEGIDGHLRLRIRFRPTFDFALHACQLSVNSQTIIAVSGSESAALCSNLPFE
                     IHGDLAESWCDISASQQIWLALSYDHSNARYDEATFAHELNRTVEHWKNWSEKSLYAG
                     PHSDLVRRSALVLKLLTYNRTGAIIAAPTTSLPERIGGDLNWDYRYTWLRDSGLILDV
                     LQRLGFHDESMRFFDWVESICMCGDIQPVYTISGERDLAEATLNHLAGYRDSKPVRIG
                     NAAAQQIQYDIYGHVLEAAWLCYSRMPRPMKPEFWEVLVRLANGAMARWQRPSHGLWE
                     TRDEPRHYVYVKLFCWVALDRAVRLAEFLRLDGALAEWRETRDQIRDYILQQGFNPKR
                     GVFTSVIGEDTTDPSLLVLALTGCVSFDDPRMKATVAAIRRSFEHRGLLYREIPQPGH
                     PWEREGAFILCTCWLIDYLVLSGKRDEGSKLLDRLISYANDLGLFAEEVDPASGNLLG
                     NFPQGFSHLGITRSILNMGGSRNPTT"
BASE COUNT         1089 a         1257 c         1384 g         1122 t
ORIGIN      
        1 tagataaacc tcgagtatcg aggcgacccg tcactatcga gatggacggc cacgcacttt
       61 aacggccacg cactttaatt gtcaggaacc cagagaacga ccgtgggaac gtgaataaag
      121 agatgcggag attctagatg cgcagaaccg aaagcgagag atatcgagga attgcgggcg
      181 actgagcaga cccgggcggc ccgtggatca accgataaac ggcggagttg gtaagccggt
      241 gatccaaggc ggcagcaagc gtcgctacaa cgcgctcgga tcgatcgccc ttggtaaccg
      301 tgaaacttgc gaccgggaga tagctgtcaa taggagggca gtggatcgtg ccgctattcg
      361 aatcgtggcc ctcggactca gagtgatgat catggccgtg cgccgaagcc gagtggtgag
      421 cgtcgtcagg tacaaaccgg attcgcctgc agctctccca tgctgcggca gtacccgcga
      481 acaacaccag aaaagaacac gtgatgaaaa ccaaacgttt catagcggcc gtcaaaaaat
      541 cttgccttat ggccgatgat ttttcaagag aacaatttac tggccggcgt ggaacagatt
      601 taccaggttt gggtcgaatg aattatccag cagcagttac cacgtcccgg ttccgaaaca
      661 gcgtgagtcg aaacggatgt catgattgtc acaggaccga acgacaatac atccttacta
      721 tctcgaacgg tccagtcaca cgccacactt tcgtaagcac ctggcgccat tccacaacgt
      781 gttaagtcgt tcttttctca gtcgcttcgc gttcatcgca acaaagctag ctaggccgat
      841 cggatccgct gaaacacgcc atttgatcat tcaatccgat tgatcggtct aggtcgatgg
      901 tctgatcgtt gcttacgact aacatggacg gttgatcatc catgtcgctc cctgctgaca
      961 atcaaactgc atccataggt ggtcgcttcc gtcgcattaa cggagttaag ccgacgctat
     1021 ccggcacaag acacaagaaa atacgaagaa aggagtaagt agttatggcg gatcagaact
     1081 gcgcacacgg aggctgcaac tgcaaggttg aacagggccg aggcgtttct agagggaatg
     1141 acacttactg tagtgatcat tgcgcaaatg caggcacagc gcgatcgggc cagtgtggtt
     1201 gcggtcattc agattgtcgg taaccgaaga gacgtgaata ttgtttaatt tttagtggta
     1261 cggtgttcaa ctaatgatcc gacgtgcagc ttgccgtcaa gagcatgact cttcggcagg
     1321 cttcaccttc gttacggggc tggcgcctgc tggaataaaa cctcagcagg caccagcgtc
     1381 ggtatcgcct cattctcccc aaactcgttg gcttaataga atgcgatagg cggtttcctc
     1441 tccgaattag cgctaaatga tctagcgcgg aggtgagtaa tggcggagca ttcatcgcag
     1501 cgccaacagc gcactcccac cgaccaacca aagaacggct ctgaacccaa ccacggcagc
     1561 atgggtaagc gcggggtagc ccggcccatg caggaaatgc cggcgcacgg gcaggcgatg
     1621 gagatgtcgc acgaagaccg gctggagatg ctcaagatgc atcaccagca aacgttatgg
     1681 atttactgga cactggtact gctcggcttc tgggttctac tttcgccgtt cacgttcggt
     1741 tacgacaaag cggtcgtcga gcccagcggc ggtcgacagg tttggttgtc gctagaggct
     1801 cgcatccagg cgatggtatg gagcgacatc ataagcggtt tggcgcttat ttttttcggc
     1861 tggcgctcgc tcactgtcaa tcgcccgatc agcttgtgga tttgttgttt catcggcatc
     1921 tggctaaaca tggcgccgct gctgttctgg tcgccatccg cggccgctta cttgaacggc
     1981 accgtaatcg gcgtgctggt gatcgccctg accattctga ttccgggcat gccgaacatg
     2041 atcatgtaca tgaaaatggg cggcgatcag ccgcccggct ggagctacaa tccctcgagc
     2101 tgggcgcaac gttggattct catcgtgctg ggttttgcgg gctgggtggt atctcgctac
     2161 cttggggcgc atcaactggg atacattgac cacgcctggg atcccttctt cggtgaaagc
     2221 acgtatcggg tattgaactc gcggatgtca cattcatggc cgatttccga tgccgccttt
     2281 ggagcgctcg cttacacctt tgaattttta atgggctata tgggagcacc ttcaaggtgg
     2341 cggacgatgc cgtggatggt cactatcttc ggaattctag taattcctct cggtttggtg
     2401 cacatttttc tcgtgatctc gcagccgctt gtagtaggag aatggtgcac gctttgtctc
     2461 ctggcggccg ccattatgct gccgatgatt ccgctggaag tggatgaggt catcgccatg
     2521 ggccagcata tgctgcaatc aaaaagaaga ggcgaagggt tatggaacgt attttggcga
     2581 ggcggcagcg ccgagggaag cgcggaagat aaacgctcgc cggagctggt gacgttgccg
     2641 gaaaaacccg gcactgtatt gaaggcatcg atctggggca tgacctttcc ctggacactc
     2701 gcactatcag ccgggctggg gatttggatc atgttcagtc ccgcgatctt tggcgccggc
     2761 aaaacgtcgt ccgatttaaa tcatctaggc ggcgctctga tcgtcgtcgt ctcagttata
     2821 gccatgggcg aaccgttgcg gcttggacga tacttgaact cattgctcgg gttaattatc
     2881 gccgtggcgg cgtggttcgc cggcgatcct ccaacggcat ttcagatcaa tagcgtgatc
     2941 atcggaattc ttgtagcgct gctcgcagtc ccgcgcgggc gcatcgtcga aagcttcggt
     3001 tcatgggaca agtatgtcag gtgaaccgga tatcggtgat tacggcctga ttggggactg
     3061 ccagagcgcg gctctagtga gtcgtggcgg cgcgatcgat tggtgctgcc ttccggattt
     3121 tgatagcccg gcaatttttt gccgcattct tgatcgggaa aaaggcggtt actgggaaat
     3181 ccagcccaag gcgccgttca caactcagcg gcgctatctt cacaacagca atgtcctgga
     3241 gacagttttc cagtgtgaaa ccggagaggc acggctgatt gatctgatgc cgattcggga
     3301 agatggaacc ggtcaatcgc tgcggatcct gcgcctgatc gaaggaatcg acgggcactt
     3361 gcggctgcga attagattca ggccgacctt tgattttgcc ctgcacgctt gccagctctc
     3421 tgttaactca caaacgatta tagccgtaag cggatcggag tccgcggcgc tttgctcaaa
     3481 cttgcctttc gaaatccacg gcgacttagc cgagtcctgg tgtgacattt cagcttcgca
     3541 gcaaatttgg ttggcgctca gttacgatca tagcaacgca cgctatgatg aggcaacgtt
     3601 tgcgcatgaa ctcaatcgga ctgtcgagca ttggaaaaat tggtcagaaa aaagtttgta
     3661 tgccgggcca cattcagatc tcgtcaggcg aagcgctctg gtgctcaaac ttctcaccta
     3721 caaccgcact ggcgcgatta tcgccgcccc gactacttct ctgccggaac gcatcggtgg
     3781 cgatttgaac tgggactacc gttatacatg gctgcgtgac agcgggctca tcctcgacgt
     3841 gttgcagcgg ttgggatttc atgatgaatc tatgcgattc ttcgattggg tcgagtcgat
     3901 ttgtatgtgt ggtgacatcc agccggttta caccatctcc ggagagcggg atctggccga
     3961 agccactctg aatcacctag cgggttaccg ggattctaag ccagttcgca tcggcaacgc
     4021 ggcagcgcag cagatccagt acgacattta cggtcacgtt ttggaggcag cgtggctttg
     4081 ctacagccgc atgccccggc cgatgaaacc ggaattctgg gaagtgctag tccgtcttgc
     4141 caatggcgcc atggcgcgat ggcagcggcc gagtcatggg ctctgggaga caagagatga
     4201 accacgtcac tatgtctacg taaagctatt ttgctgggta gcactcgacc gagctgtgcg
     4261 gcttgccgaa tttctccgtt tggacggggc tctggccgag tggcgcgaga cccgcgatca
     4321 gattcgcgac tatatcttgc aacaaggctt taaccccaag cgcggagtct tcacttctgt
     4381 gatcggggag gatacaacgg acccaagttt gctggttctc gctctgactg gatgcgtgag
     4441 tttcgatgat ccacgaatga aagcgacggt agctgcaatc cggcgctctt tcgagcatcg
     4501 cggattactt tatcgggaga tacctcaacc ggggcaccct tgggagagag aaggcgcgtt
     4561 catcctatgc acctgctggc tcatcgatta tctggtccta tccggcaagc gagacgaagg
     4621 gagcaaactg cttgaccgct tgatttctta tgccaacgat ctcggtctat tcgcggagga
     4681 ggtggatcca gcttcgggca atcttcttgg caactttcca cagggatttt cgcatctcgg
     4741 cattacccgg tcgattttga acatgggagg tagcaggaat cctacaacgt aacggtaaag
     4801 agaagccatc ctacaaaact catcgcattc aagatagagc gggtcagcga cg
//
LOCUS       DASUTG010000233         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1963950, whole genome shotgun sequence.
ACCESSION   DASUTG010000233 DASUTG010000000
VERSION     DASUTG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963950"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1904
                     /locus_tag="VF089_05225"
     CDS             192..1904
                     /locus_tag="VF089_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6173395.1"
                     /translation="MSTTKPQDSSDLYRRLVESVSDYAIFALDAKGYVSSWNPGAQHI
                     KGYTGDEIIGRHFSSFYPPEDTAWGKPAWELEVAAREGRIEDEGWRVRKDGSRFWANV
                     VISAVRNSQGEVIGFAKVTRDLTERRNTEEALRHSEERFRLLVQSVQDYAILMLDAEG
                     NIASWNEGAQQIKGYSADEILGRPFTLFYPPEAIAGGFPQRELEAAARDGRFEDEGWR
                     LRKDGSRFWANVVITALRNSEGRLVGFAKVTRDLTARREAEAQARRLAAEQASHAEAE
                     RRSVELAKLNQQLQEQAVELETQTEELRLLAETLHQKNDELQAALADAKTARENAERA
                     AAAAIDAYRELDQFAYVASHDLKAPLRGIANLAQWIQDDTGERLGAESIEHMLLLQGR
                     VHRMEALIDGILAYSRAGRRLNSPERVDTGALVREVIELLAPPPEVSIEVPSQMPAVN
                     AERVPLQQVFLNLIGNAVKHTRVERSDVIVRINWRDLGDACEFSVSDNGPGIDPEYHE
                     RIWGIFQTLAPRDKVEGTGIGLSVVRKIVETRGGSTSVESSPDGGATFRFIWPKTVRQ
                     EARA"
     gene            1901..2317
                     /locus_tag="VF089_05230"
     CDS             1901..2317
                     /locus_tag="VF089_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173396.1"
                     /translation="MSNPPLNILLVEDDEVDVMNVKRAFDRNHITNPLFVAGNGLEAL
                     DKLRCGEVPRGRRIILLDLNMPKMNGIEFLRELRNDPALAATPVVVLTTSNNDRDKID
                     AFNLNVAGYLVKPVTFAEFSELMVTLNKYWTLVEMP"
     gene            2314..>2609
                     /locus_tag="VF089_05235"
     CDS             2314..>2609
                     /locus_tag="VF089_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173397.1"
                     /translation="MSDETFKNPGAQAPTDRKVSAIGAPLRLLLVEDDELDRRAVRRC
                     LQQCGISVIAEDASSAEETLQRIASNGYDCVLLDYYIPGVKGLALFQKIREVAA"
BASE COUNT          558 a          726 c          813 g          512 t
ORIGIN      
        1 tagccataag ttagcgatgg aagcggatca attgaactgc tgagcgcgga atgttacagc
       61 gtaaagcgag ttgtatctca cccgacgcag ctcggcccga cgtcccgtta tcgcacggtg
      121 cccgggttag ctcagccggg catgtctcaa tcggcgctcg caacgtgaac gaccaaggtc
      181 gaccaagcgc aatgtctacg acgaaaccgc aagactcttc tgatttatac cgccggctag
      241 tagagagtgt gtctgattac gccatctttg cgctggatgc caaaggttat gtttcaagct
      301 ggaacccggg cgcgcagcat atcaaggggt ataccggaga tgagatcatc ggccggcatt
      361 tttcctcttt ctacccaccc gaggacactg cttggggaaa accggcgtgg gagctggaag
      421 tcgccgctcg cgaggggcgt atcgaagatg agggttggcg ggtgcgcaag gacggatcac
      481 gattctgggc caacgtcgtg atcagcgcgg tccgtaacag tcagggagag gtgatcgggt
      541 ttgccaaagt aacccgagat ctcactgagc gacgcaatac cgaggaggcg ctgcggcata
      601 gcgaggagcg cttccgactg ctggtccaga gcgtccaaga ctacgctatt ctgatgctcg
      661 acgccgaagg caacatcgca agctggaatg agggtgcaca gcaaatcaag ggatatagcg
      721 ccgatgagat tctcggccgt cccttcacct tgttctaccc gcctgaggca atcgcggggg
      781 gtttcccaca gcgtgaattg gaagcagccg cgcgcgacgg gcgtttcgag gacgaaggct
      841 ggcgcttgcg caaagacggt tcgcgcttct gggccaatgt ggtgattacc gctttgcgca
      901 acagcgaggg gcggctggtt ggatttgcca aagtcacccg tgatctcacc gcgcgtcgcg
      961 aggctgaggc gcaggcgcgc cggctcgctg ccgaacaggc ttcccatgct gaagccgaac
     1021 gtcggagcgt tgagctcgcc aaactcaatc agcagcttca agaacaagcg gtcgagctcg
     1081 agactcagac cgaggaatta cgacttttag ccgagacgct ccaccagaaa aacgacgaac
     1141 tccaggcagc gcttgccgat gccaagacag cgcgggaaaa cgcggagcgc gccgccgccg
     1201 ccgccatcga tgcctaccgg gagctcgatc agttcgcgta tgtcgcatcg cacgatctaa
     1261 aagctccgtt gcgcggcatt gccaacctgg cgcagtggat tcaagacgat accggcgagc
     1321 ggcttggcgc cgagtccatc gagcacatgc tcctgctgca agggcgcgtg caccgcatgg
     1381 aggcgctgat cgacggcatt cttgcctact cgcgtgccgg ccgaaggttg aattcgcccg
     1441 aacgggttga cacgggcgcg ctcgtgcgcg aggtgattga gttgctggcc ccgcccccgg
     1501 aggtcagtat cgaggtgcca tcgcagatgc cggctgtgaa cgccgaacgc gtgccgctgc
     1561 agcaggtttt tttgaacctc attggcaacg cggtgaaaca caccagggtc gagcgctctg
     1621 acgtaatcgt ccggatcaat tggcgggatc tcggggatgc atgcgagttt tcggtgagcg
     1681 acaatggtcc cgggatcgat ccggagtacc acgagcggat ttgggggatc ttccagacgc
     1741 tggcgccgcg tgacaaagtt gaagggaccg gcatcggcct ttcggttgtg cgaaaaatcg
     1801 tcgagacgcg gggtggctct acgtccgtgg agtcctctcc ggatggcggc gcgacatttc
     1861 gctttatctg gcccaagact gttcgacaag aggcgcgtgc atgagcaatc cacctttgaa
     1921 catcttgctg gttgaggacg acgaggtcga tgtgatgaac gtgaagcgcg cgttcgacag
     1981 gaaccacatc actaaccccc tgttcgtggc cggcaatggt cttgaagcat tggacaagct
     2041 gcgctgcggt gaggtcccgc gaggccggcg catcattcta ctcgatctca atatgccgaa
     2101 aatgaacggc atcgagtttc tccgcgagtt gcgaaacgat ccggcgctcg ccgcgacgcc
     2161 tgtggtcgtg ctcaccacgt ctaataatga tcgcgacaag atcgacgcct tcaatctcaa
     2221 cgtagccggt tatctggtga agccggttac gttcgcggaa ttttccgagc tgatggtgac
     2281 actcaataaa tattggacgc tcgtcgaaat gccgtgagcg acgagacatt taaaaatccc
     2341 ggcgcacagg cgccgaccga ccgcaaggtt tcggcgatcg gcgctccgct gcgccttctc
     2401 ctagtcgagg atgacgaact ggatcgccgc gccgtgcggc gatgtttgca acagtgcggt
     2461 atctccgtga ttgccgagga tgcaagttcg gcagaggaaa cgctgcaaag aattgcttca
     2521 aacggttacg actgcgttct tcttgactat tacattcccg gcgtgaaagg gctggctctc
     2581 ttccagaaga tcagagaagt ggcggcaga
//
LOCUS       DASUTG010000234         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1222513, whole genome shotgun sequence.
ACCESSION   DASUTG010000234 DASUTG010000000
VERSION     DASUTG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222513"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF089_05240"
     CDS             <1..248
                     /locus_tag="VF089_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173398.1"
                     /translation="RLRGKKMLHVVGEYDKGHWIEGGEKGLEFRREVYAFKRFAPYAK
                     ELRLVQVPNLTHYGHVESHNERLANIMVTGFKEYFLD"
     gene            complement(328..717)
                     /locus_tag="VF089_05245"
     CDS             complement(328..717)
                     /locus_tag="VF089_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173399.1"
                     /translation="MKKSIVIGVSLLVLGSAVTVSADDRSYGRRGDRINQRLDARGAR
                     INQHLDARGDRINDRFDARAARARADGKYRLAQRFDRQGDRIERHLDKKGDRIEAYWD
                     RRGNRIDHRLDRRDQDHGRFDHNRFRR"
     gene            910..1989
                     /locus_tag="VF089_05250"
     CDS             910..1989
                     /locus_tag="VF089_05250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173400.1"
                     /translation="MAKDKRRIQLVYLRHSFSKLWLVTIVLSLSLSLPITSNAEPLRI
                     AYTSIALVYGPLWITQEAGIFKKHGIDPEFIYIAGGPPSLQALIAGDVAISFTAGGAT
                     VAANLSGSDVVLLGASIDSLPFELWSIPRIKQPAQLRGTKLGVSRIGATTDFVARYLL
                     KKWGMQADKDVGIFQAGSGPQVFAALKSGSVQSGVLSIGPETLRAEREGYIRLADVPS
                     ELVYPFGPFAARQSFLKKQPDLVTRFMKAYVEGIHRFKTDKRTALATLEKYTKQKTTP
                     AVEKIYQVYATKHFKRVPEATLAGIQTILDELSGTRPIPPGIGPERFVESRFVRELVS
                     SGFVDDLYNDVRPRAHLPRNGAWPN"
     gene            2247..2798
                     /locus_tag="VF089_05255"
     CDS             2247..2798
                     /locus_tag="VF089_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173401.1"
                     /translation="MIVAMPYGCAGSRNLSQASRIGFEKAIDWWQKSMQTSKHKTYFI
                     VTGYTDDGGEELSARREVINKAAVDPELLNNLIEISARNEEALALKISRVRQLLPIET
                     MTVFVEARNAVSVKAIFKRKFGKTLQIRKFKARFEFNHQWITTSTSFAWFSRNWLLRV
                     WFALKKRMGRRLRKKVRFLFRSY"
     gene            complement(2898..3755)
                     /locus_tag="VF089_05260"
     CDS             complement(2898..3755)
                     /locus_tag="VF089_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6173402.1"
                     /translation="MELQQRHSRGNVFWPGNNRIAVFLSFDFQGGEDVRPDKNGRINH
                     EEYTQAEYGPNTGIWRILRILSANDVKATFLTCGGIAERYPEAVAAIIAQGHEVAGHG
                     YHHEVARDLSKEEERDVMKRTGEMLLERAQKRPVGWRSCTQSPNSLELLMEHGYLWNS
                     NSFSFDLPFVWQNGDKQLVELPRQPFGDGRTYGHRDSGNPNDTLAVWKAMFDQFYEES
                     KIAPTFCPFQFHPYISSRPGRAKTLSDLIEYMKSHEGVWFATGSEVAKWWLDNGFSAA
                     AAAQVSKVA"
     gene            3987..4988
                     /locus_tag="VF089_05265"
     CDS             3987..4988
                     /locus_tag="VF089_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173403.1"
                     /translation="MFWQSAILVLALSLASLSEAQQRTLQPLVVSYSSFTGNRAPLWI
                     AKDLGLYEKYGLDVQLVSIAAGSVSLTALLAGNVHLTTDSSSGVVGAVARGAPIVTVS
                     TNGTLPYQLIAVPSIKSAPELKGKIVGSSRIGAGTDFLLRRILAKLSFTPGKDVSLIP
                     TGVSESEKRIQLMFQGKIDATIGESDKVFQFTELSGQKISVLGEPKDFGVPSPGSEIN
                     TTRQILKDYRPRLKSFFMAYGEAIAAGRKNRELAYQAFRKYMRIDNPKLLEFTYRVQF
                     MEAIPAKPYPREDAIQASIEDLRDSTPKLEGMKAAEFFDPSMIREIDQEGFFARIEK"
     gene            4985..>5309
                     /locus_tag="VF089_05270"
     CDS             4985..>5309
                     /locus_tag="VF089_05270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173404.1"
                     /translation="MNIKIVRRLALTLLALLLWGQVPAHSQQRIRLGISAASLGFLPT
                     VLAEKKGFYSKHGINSEHVMIPCAIATNALLSGDLDYAVCTGPGIAGAIKGLPIKLVM
                     TTQDKL"
BASE COUNT         1255 a         1440 c         1384 g         1230 t
ORIGIN      
        1 ctcgcctgcg cggcaaaaag atgctccacg tggtcggcga gtacgataaa ggtcattgga
       61 ttgagggcgg cgagaaaggg ctggaatttc gccgggaggt ttatgccttc aaacgcttcg
      121 ctccgtacgc taaggagctg cggctggttc aggtcccaaa cctcacgcac tacggacacg
      181 tcgaaagtca caacgagaga ttggcaaata tcatggtgac gggttttaag gagtattttt
      241 tagattagac acacgggggt aaccggtggc gaacagagca cccaagctcc gggacgaatt
      301 cccgggcctg ggtgccaacg ttcttagtta acgacgaaag cgattatgat cgaagcggcc
      361 gtggtcctgg tcacgccgat ctaatctgtg atcgatacga ttacctcggc gatcccaata
      421 agcctcgata cggtcgcctt tcttgtctaa gtggcgctcg atgcggtcac cttgccgatc
      481 gaatcgttgg gctaatctgt acttgccatc cgctctggcc cttgccgctc tggcgtcgaa
      541 cctgtcgttg attctatcgc ctctggcatc gaggtgttga ttgatacgtg ctcctcgcgc
      601 atccagacgt tggttgatcc gatcgccgcg ccgtccatag gagcgatcat cagccgaaac
      661 cgtcacggcg ctgccaagaa cgagaaggct tactccgatc acaattgatt tcttcatgtt
      721 gtccctttct agattgacgt ggttctcaga gagttggacg cggcagagca gcaaaggttt
      781 acagctgcag ctctcgtctg gttgaactct ggggaatatc cgacctaaag tcggccatcg
      841 gcgcagcctg ctttcattac ttgacagaag gggcaatcta acttacagat tgctcacggg
      901 aacccaaaga tggccaagga caaacggaga atccagcttg tttacttgcg tcactcgttt
      961 agcaaactct ggctggtcac cattgttcta agtctctcgc taagtctccc gataacctcc
     1021 aacgcagagc cattacggat cgcctatacg tcgattgctt tggtttacgg tcctttgtgg
     1081 atcacccaag aggcgggaat cttcaagaag cacggcatcg atccggaatt catctatatc
     1141 gccggcggcc caccgtcgtt acaagccttg atcgcaggcg atgttgccat ctcctttacc
     1201 gctggcgggg ccacagtggc ggccaatctt tcgggctctg atgttgtgct gctcggcgcc
     1261 agcatcgatt ccctgccgtt cgaactctgg tcaattcccc gcatcaagca gccggcacaa
     1321 ctcaggggca caaagcttgg agtcagccgc atcggcgcaa ccacggattt tgtagcgcgc
     1381 tacctcttga agaaatgggg catgcaagcc gataaagatg tcggcatttt tcaagccggt
     1441 tccgggccgc aagtctttgc cgcccttaag agcggttcgg ttcaaagtgg cgtgctttcc
     1501 atcggtcccg agacgctgcg cgccgagcgc gaaggataca tccggctggc cgatgttcca
     1561 tctgaattgg tttatccgtt tgggccgttc gccgcgcgcc agtcatttct caagaagcaa
     1621 cccgatctgg tcacgcgctt tatgaaggct tacgtggaag ggattcaccg gttcaaaacc
     1681 gataaaagaa ccgcgctggc gacactggag aagtatacca agcaaaagac cactcctgcc
     1741 gtcgagaaaa tttaccaagt ctacgctacc aaacatttta agagagtccc ggaagctacg
     1801 ctggccggca ttcagacgat cttggacgag ctctccggca cacggccgat accgcctggg
     1861 atcggccccg agcgcttcgt ggaatcgaga ttcgttcgtg aattggtctc cagcggtttc
     1921 gtcgatgatc tctataacga cgtcaggccg cgcgcccatc tccctagaaa tggtgcatgg
     1981 ccaaattagc cgtaacaagg caaatagtcc gtaggtttaa atcagcagat gaagacagat
     2041 aacatcaccg gcaaaacgga gcgcacgcag gtgcgcctga acgaaatcac atcgtatctc
     2101 gaacagaaat agctttgcta ctggcacgga acgcctgtct gagtctggcg atcgacggca
     2161 atcggttgga tgtgacgatt taccaacccc gacctttact cgccgcataa cgcctaacag
     2221 taaaggagac cacgaccatt tcgattgtga tcgtagcgat gccctacggc tgcgccggtt
     2281 caaggaatct ttcacaagca tcacgcatcg gctttgagaa ggccatcgac tggtggcaga
     2341 agtcgatgca gacttccaag cacaaaactt attttatcgt caccggttat accgacgatg
     2401 gcggcgaaga actttcggcc cgcagggaag tcattaacaa agcggcggtg gacccggagc
     2461 tattgaacaa cctaatcgag atcagcgcgc gcaacgaaga ggcgctggcg ctcaagatct
     2521 cgcgcgtgcg ccaattattg ccgatcgaaa cgatgaccgt tttcgttgaa gcgcgcaacg
     2581 cggtcagcgt taaagcgatc ttcaaaagaa aattcggcaa aactcttcag atcaggaaat
     2641 tcaaagcgcg gtttgaattt aatcaccagt ggataacgac ctccacctct tttgcctggt
     2701 tctccaggaa ctggctgctg cgggtctggt tcgcgctgaa aaagcggatg gggcgccggc
     2761 tgcgaaagaa agtacgtttt ctatttcgat cgtattgata acggcggggt ggcgtagggg
     2821 cgaacgtttc agttcgccct tacggctgga gtgcccgatg cttgtgacgc ttggcaagcc
     2881 gctacaagcc cattacttta ggctaccttg gaaacttgcg ctgccgcggc ggctgaaaaa
     2941 ccgttatcga gccaccattt tgcaacttca ctaccggtcg caaaccaaac gccctcgtgg
     3001 gatttcatat attcgatcaa atcagacagt gtcttggctc ggccgggccg gctagaaatg
     3061 tacgggtgaa actggaacgg gcagaacgtc ggcgctattt ttgattcttc atagaactgg
     3121 tcgaacatag ccttccacac cgctagagtg tcgtttggat tgcctgaatc tcggtggccg
     3181 taggtcctgc cgtcgccaaa cggctgtcgc ggtagttcga cgagctgctt gtctccattc
     3241 tgccaaacaa acggtaaatc aaacgagaaa gaattgctgt tccagagata gccatgttcc
     3301 atcagcaatt ccagactatt cgggctttgc gtgcacgaac gccatcccac cggccgcttt
     3361 tgcgctcgct cgagcagcat ttcacccgtt cgcttcatca catcgcgctc ctcctccttg
     3421 ctcagatcac gggcaacctc atgatggtag ccatggcccg ccacttcgtg cccttgagcg
     3481 atgatcgccg caaccgcttc ggggtacctt tccgctatgc caccgcaggt tagaaatgtt
     3541 gccttaacat cgttggccga caggatgcgc agaatgcgcc agataccggt attgggaccg
     3601 tactccgcct gagtatactc ttcatgattg atcctgccgt ttttgtccgg ccgaacatcc
     3661 tcaccacctt gaaaatcaaa gcttagaaac accgcgatgc gattgttgcc gggccagaat
     3721 acattgccac gagaatgacg ttgttgcagc tccatttgaa cctcctaagt tcgcctacct
     3781 tattgatctg atgagttctc cacactcttc accgaggtga tgtctacatt tactaaagtt
     3841 cttttagtcc acccgcgcta gccttgcaac ctgaatgcgc gacgttgttg ctatgaagaa
     3901 aaggatgtat agaaaggacg cgtagccgga ataccacaac cagagactat cattcttaac
     3961 catgagttca cgccatagga ggttcgatgt tttggcaaag tgctattctg gtgttagcgc
     4021 tgtcgcttgc gagcttaagc gaggcgcaac aacgaacgct tcaaccgctg gttgtctcct
     4081 atagttcctt caccggcaac cgcgcgcctt tgtggatcgc caaagatctc ggactgtacg
     4141 aaaaatatgg gctcgatgta caacttgtca gcattgccgc tggcagtgta tccctcactg
     4201 ctttactcgc cggcaatgtc catttaacca ctgattccag ttcgggtgtg gtgggtgcgg
     4261 tcgcccgcgg cgcgcccatt gtgacggttt ccaccaatgg cacattaccg taccagctga
     4321 tcgcggtgcc ttcgatcaaa tccgccccag agctgaaagg taaaatcgtc ggcagcagcc
     4381 gcatcggcgc cggcaccgat tttctcttgc gccgaatcct cgccaagcta agcttcaccc
     4441 caggtaaaga cgtttcgctg attcccacag gcgtgagcga atcggagaag agaatacaac
     4501 tgatgttcca gggtaagatc gatgcaacta tcggcgaatc cgacaaagtc tttcaattta
     4561 ctgaattgag cgggcaaaag atctcagtgt tgggcgaacc taaggacttt ggcgttcctt
     4621 caccggggag cgagatcaat accacccgcc agattctgaa agattatcgc ccacggctca
     4681 agagtttttt catggcttat ggcgaggcaa tcgcggccgg cagaaagaac agagagcttg
     4741 cctaccaggc attccgcaaa tacatgcgga tcgataaccc gaagcttttg gagtttacct
     4801 acagagttca gtttatggag gcgattcccg ctaagcccta tccaagggag gatgccattc
     4861 aggcgagcat cgaggatctc agggattcga cccccaaact cgaagggatg aaggctgcag
     4921 agttttttga tccgtcgatg atcagagaaa tagatcagga aggttttttc gcgagaatag
     4981 aaaaatgaac atcaaaattg tcagaagact ggctttaacg ctcctcgccc ttcttctctg
     5041 ggggcaagtg ccggcgcact cgcaacagcg aattcgtctt ggaatttccg ccgcatcgct
     5101 gggctttctg ccaacggtgt tggcggaaaa aaaaggattt tacagcaagc atggaatcaa
     5161 ctccgagcat gtgatgatcc cttgcgcgat tgccacgaac gctcttctgt ccggcgattt
     5221 agattacgcg gtctgcaccg gacccggcat tgcaggtgcg atcaaagggc tgccgataaa
     5281 actagtgatg accacacaag acaaactag
//
LOCUS       DASUTG010000235         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2113371, whole genome shotgun sequence.
ACCESSION   DASUTG010000235 DASUTG010000000
VERSION     DASUTG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113371"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF089_05275"
     CDS             <1..232
                     /locus_tag="VF089_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173405.1"
                     /translation="KRCTLAQLSLAWILHQGDNFVPIPGTRRITHLEEDAAAAEIVLT
                     SDELAEIDKLFPRQGAASGARHDYDRSKELNI"
     gene            complement(395..910)
                     /locus_tag="VF089_05280"
     CDS             complement(395..910)
                     /locus_tag="VF089_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173406.1"
                     /translation="MEKMPIVNLLEETRQLSESGQRVRVLWQHPDTLVFIARGREYRS
                     EFHINPSDETMYMIKGEMRLHYRTPQGKEEVAVIPEGAMIYTPAGTPHSPRFATDAFA
                     LISERKRRGNEIDRFHWYCQKCDALLHEETFTVKDYADDPVSKAYAKFFGSTEFRTCG
                     CGEVMPAPEAI"
     gene            1044..2336
                     /locus_tag="VF089_05285"
     CDS             1044..2336
                     /locus_tag="VF089_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173407.1"
                     /translation="MHYIYEGVKLVKLFTRRITVQRLWNFRTLRSLRHRNFRLLMEGA
                     LISSSGDFMQNIAQSWLVWELTRSPVALGVIAFFDTLPRLLVGAVGGAIADRFDRRRV
                     LQVTQALAMTQAIIYWLAVEFDFIEFWHIAGLAFFLGLVNTINQTARQSLVNSLVPKE
                     ELLNAIGLQSSVFNFSKILGPSLGGVIIAFIGIAGCFLINALSFVALLFNLYLMDLPP
                     WEKRRDKQSLVEDIGEGFRYVRDNRRILYIVGISYVVALFGAPYNRFVPVFATNILQV
                     GPTGFGLLMAAPGLGATIASLSLATVKKLRVGVHAICYCVLGFAFSLILFAFSQSFVL
                     SLFFLAMVGFSQIAERALSNTAIQIATPPHLLGRVLSLFFMDRGLWSLGSVMIGTAAS
                     AIGVNWSFGVCGAVCAIAASSLLFVTRRGPVSNVASQA"
     gene            complement(2599..2985)
                     /locus_tag="VF089_05290"
     CDS             complement(2599..2985)
                     /locus_tag="VF089_05290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173408.1"
                     /translation="MVEMKNLVKEADANPGINHLSLWRTDLAYFWVINCEPKIQDDMH
                     YHENDDHIFMVIEGECTVRTPRKEFVLRQFDTVLLKSGESYQLCNTGTGRMLLLGAGN
                     AGVNGQPRTRVPKIPSHIPLREPIVA"
     gene            complement(3142..>3462)
                     /locus_tag="VF089_05295"
     CDS             complement(3142..>3462)
                     /locus_tag="VF089_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6173409.1"
                     /translation="SREYCSSPASIQKEIVMALSYEDLIEPAIVKQVSKTVAYCRCTR
                     TKNPPFCDGSHAATNIKPHILEFAEPTTIAICRCWRSKDHPYCDGTHGRLVKPKERPQ
                     KKHE"
BASE COUNT          700 a          884 c          944 g          934 t
ORIGIN      
        1 gaagagatgc accttggcac agctatctct cgcatggatt ctgcaccagg gagataattt
       61 tgtgcccatt ccgggaacgc gccggatcac gcatttggaa gaggatgccg ctgcggcaga
      121 gatcgtatta acgtcggacg aattagctga aatcgacaag ctgtttccgc gacaaggcgc
      181 ggccagcggc gcgcgtcatg attatgaccg ctcgaaagag ctgaacatct gaaggatcct
      241 ttaagcagtt caacagttca atgttcaaag attcaaggta taaagacgcc ctccgattct
      301 ttcgccacaa ggggaggaaa gagaacttgc gccgagactt catccggatt gagcgttgaa
      361 cgttgaacct ttgaactgtt gaacgaattc gagatcagat tgcctccggc gcaggcataa
      421 cctcgccgca tccgcaggtt ctaaattctg tgctgccaaa gaatttagcg taggctttgg
      481 agaccgggtc gtcagcatag tccttgaccg tgaacgtctc ttcgtggagc aaggcatcgc
      541 atttctggca gtaccagtga aaccggtcga tttcgttccc gcgcctcttc cgttctgaaa
      601 tcaacgcaaa cgcatcggtg gcaaaccggg gagaatgcgg tgttccggca ggagtataga
      661 tcatcgctcc ttcggggatc acggcaactt cttctttgcc ctgcggcgtg cggtaatgta
      721 accgcatttc acctttgatc atgtacattg tctcgtcact tggattgatg tgaaactcgc
      781 tgcggtactc ccgcccgcgc gcgatgaaga ctaaagtgtc cgggtgctgc cataatactc
      841 gaacacgttg gccgctttcc gacagctgcc gggtctcctc cagcaaattg acaatcggca
      901 tcttctccat tggttatctc ctttggccac gccttagaaa aaatataata agtcgatcgc
      961 cggcttcata caatttgctg cagcatacag tcaagctggt gtaagtgctg taaatcctac
     1021 tgaggcagag tccgaacgag tctttgcatt acatctacga aggcgtcaaa ctggtgaaac
     1081 tttttacccg tagaattacc gtgcaacgtc tttggaattt tagaaccctg cgatcgctca
     1141 ggcatcgtaa cttccggctg ctcatggaag gcgccttgat ctcgagctct ggcgatttca
     1201 tgcagaacat cgcccagagt tggctcgttt gggagctgac ccgttcgccg gtcgctcttg
     1261 gagtgatcgc atttttcgat acccttcccc gcctcctggt tggggcggtg ggtggcgcga
     1321 ttgcggatcg cttcgaccgt cgccgggtat tgcaagttac tcaagcgttg gcgatgacgc
     1381 aggcgattat ctactggcta gcggtggagt tcgatttcat tgaattttgg cacatcgccg
     1441 gtctggcgtt ctttttaggt ttggttaata cgattaacca aacggcgcgg cagtcgctgg
     1501 tcaatagcct ggtgcctaag gaggaattgc tcaacgccat tggcctgcaa tcttcggttt
     1561 tcaatttctc caaaatttta gggccttccc tcggcggcgt gatcatcgcg tttatcggca
     1621 ttgccggctg ttttctgatc aatgcgctga gctttgtcgc gcttttgttc aatctctatc
     1681 taatggatct gccgccgtgg gaaaagcgcc gggacaagca aagcttagtg gaggatatcg
     1741 gagaaggatt ccggtatgtc agagacaatc gccgcatcct ttacatcgtc ggtatctctt
     1801 acgtggtggc tctgttcggt gcgccgtata atcgcttcgt gccggtgttt gccacgaaca
     1861 ttctacaggt cgggccgact ggattcggcc tcctgatggc cgcgcctgga cttggcgcga
     1921 cgatagcatc cttgagtctt gctacggtga agaagctgcg cgtcggcgtt cacgcgatct
     1981 gttattgtgt tctggggttt gcgttttccc tgattctgtt cgccttttct cagtcatttg
     2041 tcttgtcact tttttttcta gcgatggtag gatttagcca gatagccgag cgcgctctgt
     2101 ccaacacggc tattcaaatt gccacgccgc cgcatctgct ggggcgagtt ttaagcttgt
     2161 tctttatgga tcggggcttg tggtctctgg gcagcgtcat gatcggcaca gccgcttcgg
     2221 cgatcggcgt caattggagc tttggcgtgt gcggtgcagt ttgcgcgatc gccgcgtcct
     2281 cattgctgtt cgtgacgcgc agagggccgg tctctaacgt agcgtctcag gcctgatgcc
     2341 gacacacctg ctttgggtgg gttggatcgt ttgatacgcg atcccgtttc gcactctacg
     2401 agacatgaca aacttaacga aagccttcga gatctgttga agtcgtcctg cggcttcgcg
     2461 cctaaatgag cagattaccc gagcaggtat gtgactggag cagacggcgg tccagcaatc
     2521 aattgcgccg gacgaccgtc ggaccacgag tgctcctttc ccaccgatgt ttgtgaggag
     2581 gtaagcgcga cgccagcatc atgcgacgat gggttcgcgt aatgggatat ggctgggaat
     2641 tttcggcacg cgcgtgcgcg gttggccatt gacgccggca ttgcctgctc ccaagagaag
     2701 cattctgccg gtaccagtgt tgcagagttg ataagattcg ccggatttta acagcactgt
     2761 atcgaattgt cttaaaacga actctttacg cggtgtgcgt acggtgcatt cgccttcgat
     2821 taccatgaat atatgatcgt cgttttcatg atagtgcatg tcgtcttgaa tcttgggttc
     2881 gcagttgatt acccagaagt aagccagatc ggttcgccat aggctcaagt ggttgatccc
     2941 cgggttagcg tcggcttcct taacaagatt tttcatttcg accatatcgt tcctccgcta
     3001 aatttacatg ccgatgaaaa cgctccatct acttcgttgg gcttctccga tccacgccga
     3061 cctacgtcga gtacgtctcc gccgaccaat tatggcgcct cggagctgga acgttttgat
     3121 cagcctgata ggacattgtt tttattcgtg cttcttttgt ggtcgttctt tgggctttac
     3181 cagccggccg tgagtgccgt cgcaatacgg atgatccttt gatctccagc agcggcagat
     3241 ggcaatggtc gttggctccg cgaattccag tatgtgcggt tttatgtttg tggcggcatg
     3301 cgaaccgtca cagaaaggcg ggttcttggt ccgggtgcac cggcaatagg ccacggtctt
     3361 ggacacttgc ttgacaatcg ccggttcgat gagatcttcg taagacaggg ccataacgat
     3421 ttctttctga atgcttgcag gagatgaaca atattcccgg ct
//
LOCUS       DASUTG010000236         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_333860, whole genome shotgun sequence.
ACCESSION   DASUTG010000236 DASUTG010000000
VERSION     DASUTG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333860"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF089_05300"
     CDS             <1..409
                     /locus_tag="VF089_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173410.1"
                     /translation="ASDKALAAGGVLLANAEDYVPGWPLTCGWTLRRWLLEHRDLAVR
                     FIRAWAAATDWLLAPQNRAETLELIMAKEGLNRDSAERAYRKVVPQARINPAALNTVI
                     ELRKEMGVYKPPYASPERFYDASYWREAMKQAD"
     gene            592..1614
                     /locus_tag="VF089_05305"
     CDS             592..1614
                     /locus_tag="VF089_05305"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX6173411.1"
                     /translation="MVNTQELETVAQGMVAKGKGILAADESMGTIKRRFDSIKIESNE
                     ENRRAYREMLFTTKGIEEAISGVILFDETLRTKSSDGTPFPQLLSKKGIIPGIKVDKG
                     PVDIPNFPGEVVTEGLDGLRGRLKEYKELGAKFAKWRAVISIGDGIPTYTCLELNGEA
                     LARYAALCQEAGIVPIVEPEVLLDGSHTIERAQEVTEHTLKVTFTQLFLQRVYLEGMI
                     LKPSMVVPGKDSSKKASAEEVAERTIRCLKRTVPAAVPGIAFLSGGQSAESATEHLNA
                     MNAMGPLPWEVTFSYARALQDPALKAWKGDPGNISAAQKIFYHRAKCNSAARSGSYNK
                     QMEAAA"
     gene            complement(1758..>2405)
                     /locus_tag="VF089_05310"
     CDS             complement(1758..>2405)
                     /locus_tag="VF089_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173412.1"
                     /translation="ESIDPKLGPRYRNVMIGILYNKSIIKPGDAPKSLEDLLKPQYRG
                     KLVMADPTQHTTTTQWVASLEKLLGKDKADKFIRDLAATKPIMVESLLPAAERVASGE
                     TPVGITYVKYVHTFGERGAPIDYVRLGKMMGDGHYLTLGNKAPHPNAGKAFIDSFLNA
                     ESMTILAKQGEFVNRKGIYPPIADAEKIQFVEMHDLDSKAFAQKKKEYQEIFLRK"
BASE COUNT          567 a          621 c          696 g          521 t
ORIGIN      
        1 ggcttccgac aaggcgttgg cagcgggcgg agtgcttctc gccaacgcgg aagactacgt
       61 tccaggttgg ccgctgacgt gcggctggac attgcgccgc tggctacttg agcatcgcga
      121 tctggccgtg cgctttatac gcgcgtgggc ggcggcaacg gactggctgc tggcgccgca
      181 aaatcgggcg gaaaccctcg aactaataat ggccaaagaa gggctcaacc gggattccgc
      241 agagcgggct tatcgcaagg tcgtgccaca agcgcggatc aatcccgctg cattaaacac
      301 ggtcatcgag ctaagaaagg aaatgggcgt gtacaaaccg ccgtatgctt cgccggaacg
      361 attttatgac gcgagctact ggcgtgaagc gatgaagcaa gctgattgaa aacgtgttcg
      421 ttcatcggag aagtaccctg ctcggttggg agaagcgacg ctctaccacg aacacgatta
      481 ttaacggctt gccatgcccc gttcacaatg taaaatggac tgcaatcgaa tcggttcatt
      541 taaaaatatc tcgaccaaaa agtttcaagg ctaaataagg aggcaccaat tatggttaat
      601 acacaggaac tggaaaccgt tgctcaaggc atggtggcaa aagggaaggg gatattggct
      661 gccgacgaaa gcatgggaac tatcaagcga cgctttgaca gcatcaagat cgaatccaac
      721 gaagagaacc gccgcgctta tcgcgagatg ttgtttacaa cgaagggcat cgaagaagcg
      781 atcagcgggg tgattctctt cgatgaaaca ttgcggacga aaagcagcga cgggacgccg
      841 tttccgcaat tactgtcgaa aaaaggcatc atccccggca tcaaagtcga taaaggcccg
      901 gtggacattc ccaattttcc cggcgaagtt gtgacagaag ggttggacgg actccgaggc
      961 cggctgaagg agtacaagga actcggcgcg aaattcgcga agtggcgcgc ggtgatctcc
     1021 atcggcgacg ggatcccgac ctatacgtgc ctcgagctca atggcgaggc gctagcccgg
     1081 tacgcggcac tgtgccagga ggcgggcatt gttccgattg tcgaaccgga agtgttgctc
     1141 gatggctcgc acaccatcga acgggctcaa gaggtgacgg agcatacgtt gaaagtaacc
     1201 tttacacagc ttttcttgca gcgggtttat ctcgaaggaa tgattctcaa gccgagtatg
     1261 gtggttccag gaaaggactc ttcgaagaag gccagcgctg aagaagtggc cgagcgcacg
     1321 attcgttgcc tgaagcgcac cgtcccggca gcggttccgg gaattgcgtt tctatcgggc
     1381 ggacagagcg ccgagagcgc gaccgaacat ctcaacgcca tgaacgccat ggggccgctt
     1441 ccgtgggagg ttacgttttc ctacgcgcgg gctctccagg atcccgcttt gaaagcctgg
     1501 aaaggcgatc ccggtaatat cagcgcagcg caaaaaatct tctatcatcg ggccaaatgc
     1561 aacagcgcgg cccgaagtgg cagctacaac aagcagatgg aagcggctgc ttagaatcgg
     1621 ctggcgatta cagaggcagg agcgaagaga ggcggtcacc cgggagtgac cgtttctctt
     1681 ttcagtcttg atggttaaac gctgttcccg aattccgtcg ctctactaat cgataacctg
     1741 agtgccggat caaattctca cttacgtaga aagatctcct gatactcttt tttcttctgt
     1801 gcgaaagcct ttgagtcgag atcgtgcatt tcgacgaact gaattttttc cgcatcggcg
     1861 atcggcggat aaattccttt tcgatttacg aattcccctt gtttagcgag aatcgtcatg
     1921 ctttccgcat tgaggaagct atcgataaaa gccttgcctg cattcgggtg cggcgccttg
     1981 ttgcccaatg tcagatagtg accgtcgccc atcatcttgc cgagcctgac gtaatcaatg
     2041 ggcgcgcctc tctcgccgaa ggtatgaacg tatttaacat aggtgatgcc gaccggtgtc
     2101 tcgccgctgg cgacgcgctc cgccgccggc aacagagact cgaccatgat cggtttggtt
     2161 gcggccaagt cgcggatgaa tttatcggct ttgtccttcc ccagcagctt ttccaggctg
     2221 gcgacccact gagtcgtggt cgtgtgctgg gtcggatcgg ccatcaccag ctttcctcga
     2281 tattgcggct taagcagatc ttcgagcgac ttgggcgcat cgccgggttt gataatgctc
     2341 ttgttataaa gaatgccgat catgacgttg cggtaccggg gacccagttt gggatcgatc
     2401 gactc
//
LOCUS       DASUTG010000237         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1519917, whole genome shotgun sequence.
ACCESSION   DASUTG010000237 DASUTG010000000
VERSION     DASUTG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519917"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VF089_05315"
     CDS             <1..1248
                     /locus_tag="VF089_05315"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640428.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEX6173413.1"
                     /translation="TNGFADGIVVTPSHNPPADGGFKYNPPNGGPADTDVTDWIQQRA
                     NDLLKNANREVKRISYHAAVRAANIHQEDLLTPYVDDLIEVIDMEAISAGLVKIGIDP
                     LGGAAVSYWQAIGERYRLDLTVVNTQIDPTFKFMTLDHDGKIRMDCSSPYAMASLVAL
                     KDQYQIAMGNDADADRHGIVTPGIGLMNPNHYLAVAVDYLLANRSDWNPRASVGKTLV
                     SSSLIDRVVDLRGRKLFEAPVGFKWFSQGLLDGTICFGGEESAGASFSCRNGSVWTTD
                     KDGIILSLLAAEIIARTGKDPAQHYEELARDLGRPYYARTDVPASAAQKAALKKLRPE
                     ALSTQDLAGETITAKLTRASGNNAEIGGLKVVSQNGWFAVRPSGTEDLCKIYAESMRS
                     EEHLKQIQDEAWQILQAVFTASA"
     gene            complement(1265..1359)
                     /locus_tag="VF089_05320"
     tRNA            complement(1265..1359)
                     /locus_tag="VF089_05320"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1323..1325),aa:Sec,seq:tca)
BASE COUNT          445 a          535 c          573 g          477 t
ORIGIN      
        1 acaaatggtt ttgccgacgg catcgtcgtg acaccgtcgc ataatccgcc ggcggatggc
       61 gggtttaagt acaatccgcc caacggcggc ccggccgata ctgacgttac cgactggatt
      121 cagcaacggg ccaacgatct attgaagaac gccaatcggg aagtaaaacg gatctcctat
      181 catgccgccg tgcgggcagc caacattcat caagaagatt tgttgacgcc ctatgtcgat
      241 gatctgatcg aagttatcga catggaagca atcagcgccg gtcttgtaaa aatcggtatt
      301 gaccctcttg gcggcgcggc ggtttcttac tggcaagcca tcggcgagcg ataccggttg
      361 gacctcacgg tggtcaacac tcagatcgat ccaactttta agtttatgac gcttgatcac
      421 gacggtaaaa tcaggatgga ttgttcgagc ccatacgcca tggcgagctt ggtggcttta
      481 aaggatcaat accagatagc gatgggcaat gatgccgacg ccgatcgcca cggcattgtc
      541 acgccgggca ttgggctgat gaatccgaat cactatcttg cggtggctgt tgattatctc
      601 ctagccaacc ggtcggattg gaatccccgc gcttcggtgg ggaaaactct ggtgagcagt
      661 tcgctgatcg accgagtagt ggacttacgg gggcgcaagc ttttcgaggc gccggtgggt
      721 ttcaaatggt tttcacaagg gttgctagat gggacgattt gctttggcgg cgaggaaagc
      781 gccggcgcca gtttttcatg ccgcaacgga tcggtttgga ccaccgataa ggatggaatc
      841 atccttagcc tgctcgccgc ggagatcatc gcgcggacgg gaaaagatcc ggctcaacac
      901 tatgaagagc tcgctcgaga tctcgggaga ccttattatg cgcgcaccga tgtcccggca
      961 agcgcggcac aaaaggcggc gcttaaaaag ctccgtccgg aggcactatc gactcaggat
     1021 ttggcaggcg aaaccatcac ggcgaaactg acgcgagcat caggcaacaa tgctgaaatc
     1081 ggcgggctca aggtcgtttc acaaaacggc tggtttgccg tgcgtccgtc gggaactgaa
     1141 gacttgtgca aaatttatgc ggaaagcatg cgcagtgagg aacatctcaa gcaaatccaa
     1201 gacgaagcct ggcagatcct gcaagcggtt ttcacggcga gcgcatgaca agtgacggta
     1261 tatctggcgg agaagggagg gagtcgaacc ctccgggaac cttacggccc ccaaactggt
     1321 tttgaagacc agcggcacca ccgggcacct tccttctccc tctgagtttt agctgatctg
     1381 tgctgcgttg ctttttctgg ttgccgttct gtacatctta accgttaccg ggcggctgac
     1441 tgtggaggcg atagttcgtc gtcagagtcc acatttatgc atctgaaccg atgtgagggc
     1501 tgcgcatgac aacgttgatt gcctggcgag ccaatgagtc agcgcggtat tgcggtagtt
     1561 atggaaacca aacttgcgaa cctctgtcaa ccatcatcgg cccaatgtgg tatctcgatc
     1621 gagctcggag tgctaagcct gcccgcagga ttcctgctgt gcgcgccgcg ccgtagggcg
     1681 ttctgttcct ttgcacagtt tcgcgcgcgt caatgagttg ggctattttg ctttcgctct
     1741 tgtcaggcga ttccaacaac cgcagccggt cagcaatttt ccgactctac gtgtgtgctt
     1801 ccgccccttt caaggattcc gtcccgccgt cgaattcctt gctcgttgcg cgaacattga
     1861 tttattttct atgtgtattc cgaagcccgg tgaagcttta tagtctgacc gccggtacag
     1921 tcgcgccaaa tctcgtggag aggtataaat aagcctgtag cccgcgttga gctgcctgtt
     1981 aagatcgcta gcgtgccgca attcttgctc tatgcttcta gtgctcatca
//
LOCUS       DASUTG010000238         2841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2113843, whole genome shotgun sequence.
ACCESSION   DASUTG010000238 DASUTG010000000
VERSION     DASUTG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113843"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VF089_05325"
     CDS             complement(<1..385)
                     /locus_tag="VF089_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173414.1"
                     /translation="MKRKKRFPIIPTLGITLGLILLGSVHRAGAEEFYQGKTIRFIVG
                     FSAGGGFDTYSRMIARHIGKHIPGNPNTVVENRTGAGSLVAANYIYRKAEPDGLTIGN
                     FIGPLVLQDAIGNKAAQFDGRKFGWL"
     gene            complement(441..1541)
                     /locus_tag="VF089_05330"
     CDS             complement(441..1541)
                     /locus_tag="VF089_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6173415.1"
                     /translation="MPGRNDFQDAHLWQITAARDLLVVVLIGFVLWLLYLLGDIFIPL
                     LIALVLAHVFNPLITWLERKWDWPRPLTAALLLAIVILGSWALFAWLGPLLVSQITDL
                     AQRLPGYLRTLAANYGIRVTELESAIQQYQIDIQRILGQIFQTTGQAVGFVTTVFGFA
                     LNWIFSIALVLVYFFFFAWGFNKATHALTQYLPQSHRERILDLASKMSQAVSHFFRGR
                     LVIACIMGGLLSIGWFWTGVPYWFFLGMLTGLLNIVPYLSVLTWPVAVLLKYLDEMNS
                     SAGSPSFVSAAVWPTVVYVVIQVSEGWFLTPWVQSGQTNLNAATVLLVVIIGGALGGV
                     WGLLFAIPIAACIKILVTEVVLPPLRSWAAKH"
     gene            complement(1636..2415)
                     /locus_tag="VF089_05335"
     CDS             complement(1636..2415)
                     /locus_tag="VF089_05335"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX6173416.1"
                     /translation="MGRKFLLVLIAAVLAPAAFYLWRELNKDQRQAAPAPAMVPEPSV
                     RYPVEDLGPMADSLPPLSESDDTVHRAVAALFGGGFERLVISKDIVRRVVATIDNLSR
                     DQVSAQLMPVKPAGGLTLTMETDAGVAIARENAARYLPYVRIALAVPTDKLAALYVRF
                     YPLLQQQYEALGYPDGYFNDRVVQVIDHLLATPDVREPLVLVQPRVLYEFADPTLQNL
                     SAGQKILLRMGAENRQQAKAKLREFRQAITAENAAGDLRGP"
     gene            complement(2621..>2841)
                     /locus_tag="VF089_05340"
     CDS             complement(2621..>2841)
                     /locus_tag="VF089_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173417.1"
                     /translation="GFIPASAIATFLYLKLGAGERWPVTLAITAGCWLFFFGLFDYAL
                     QMPFPQGALFDWVEIDVAAVQSLFAAVR"
BASE COUNT          698 a          808 c          752 g          583 t
ORIGIN      
        1 cgagccagcc aaactttcgc ccgtcgaact gcgcagcttt gttgccaatg gcatcttgca
       61 gcaccaacgg accgatgaag ttgccaatcg taagaccgtc aggttccgcc tttctgtaga
      121 tgtagttcgc cgcaaccagg cttcccgcgc cggtcctgtt ctccactaca gtattgggat
      181 tgccggggat gtgtttgccg atatgacgcg cgatcatcct ggagtaggta tcgaagccgc
      241 caccggcgga aaagccgacg atgaatcgaa tcgtctttcc ctgataaaac tcttcggcgc
      301 ccgctcgatg cacgctgccg agcagaatca gaccgagagt gatacccagt gtcgggatta
      361 ttgggaaccg tttcttgcgc ttcataaaca ttctcctttc cggtcgacga tattattaaa
      421 atatgctccg acttcactcg ctaatgcttt gccgcccacg atcgtaaagg cggcaagacc
      481 acctcggtta ctaggatttt gatgcaggca gcgataggga tcgcgaacag aaggccccac
      541 acgccgccca aggcgccgcc gataatgacg actagcagga cggttgcggc attcagatta
      601 gtttggccac tctgaaccca cggcgtcagg aaccatcctt ccgagacctg aatcaccacg
      661 taaaccacgg tgggccatac ggccgccgag acgaaactgg gactgcctgc gctgctgttc
      721 atctcatcca aatacttgag caggacagca acaggccacg tgaggaccga cagataagga
      781 acgatgttca aaagccccgt cagcattccc agaaaaaacc agtaagggac gccggtccaa
      841 aaccagccga ttgagagcaa cccgcccatg atgcaggcga tgacgagtct gccgcgaaag
      901 aaatggctaa ccgcttggct catcttactt gcgagatcga ggattctctc cctgtgactt
      961 tgaggcaggt attgcgtcag agcgtgcgtg gctttattga agccccaggc aaaaaagaaa
     1021 aagtaaacaa gcaccagggc aattgaaaag atccagttta gagcaaagcc aaagaccgtg
     1081 gtgacaaagc caaccgcctg tccggttgtt tggaaaattt gccccaggat gcgctgaatg
     1141 tcgatctgat actgctgaat agccgattcc agctcagtga ctctgatgcc gtaattagct
     1201 gccaaagtgc gcagataacc tgggagccgc tgcgccaggt cggttatctg cgagacgaga
     1261 agcggtccca accaggcaaa taaagcccaa ctgccgagta tcactatagc cagcaacagc
     1321 gccgccgtca gtggccgtgg ccagtcccat ttgcgctcga gccaggtgat cagtgggtta
     1381 aagacgtgtg ccagtaccag cgcaatcagc agcggaatga aaatgtcgcc tagcagatac
     1441 agcagccata gaacgaagcc gatcagaact acgactagca aatcgcgagc tgcagtgatc
     1501 tgccagagat gcgcgtcttg gaaatcattt ctaccgggca tgatgagaca tcctacgacg
     1561 ttcactgtag cggaaactga aaatactcgc ggccggaagg tgtcgccatg acggtcgcaa
     1621 ctcggtagtg tgtattcatg gaccgcgcaa atctcctgcc gcgttttctg cagttatcgc
     1681 ttggcgaaat tcccgcagtt tcgcttttgc ttgctgcctg ttctccgcac ccattcgcag
     1741 caggatcttc tgtcccgccg agagattttg cagtgttgga tcggcaaatt catacagcac
     1801 ccgtggctgc accagcacaa gaggttccct gacatccggc gttgccagta aatgatcgat
     1861 cacctgcacc acgcgatcat tgaaatagcc gtcaggataa cccaatgctt cgtactgctg
     1921 ctgcagtaag ggatagaagc gtacgtagag cgctgcaagt ttgtcggtcg gaacagctag
     1981 cgcgatacgg acatagggca agtagcgcgc ggcattctct cgcgcgattg cgacacccgc
     2041 atccgtctcc atcgtaagcg taagtccgcc cgcgggcttt accggcatca gctgcgcgga
     2101 cacctgatcg cgggataagt tgtcgatggt tgcgacaact cggcggacga tatcctttga
     2161 aatgacaagc ctctcaaagc ctcccccgaa cagcgcggct acggcacgat gcaccgtgtc
     2221 atcactctcg cttagcggcg ggagcgagtc cgccatcggt ccgaggtctt cgacgggata
     2281 tcgaaccgaa ggctcgggca ccatagcggg ggctggcgcg gcctgccgct gatccttgtt
     2341 gagctcgcgc cataagtaga aggcagcggg agcaagcact gctgcgatta gtaccagaag
     2401 gaatttccgt cccattagca tgaatctcct tccatctgca gcgactcagt atttctcgta
     2461 agacataacg gccgcgctga gccggctttc aacctcaatg cgaatggctt ggcgacaccc
     2521 caaaatcctt ggtgccgcca gacaatgatt atcgtcttac cgatctgtga tcgttttgct
     2581 ttttgcatgc ctccctgcgg caagcgttgt tctaaattca tcaacgtaca gccgcgaaaa
     2641 gactttgcac ggctgcgaca tcaatctcga cccagtcgaa aagtgcgccc tgcggaaacg
     2701 gcatctgcaa cgcataatcg aatagaccga agaaaaatag ccagcagccg gctgtaatcg
     2761 ccaaggtcac aggccatcgt tcgccggcgc ccagcttcaa gtaaagaaag gtcgcaatcg
     2821 ccgaggccgg gataaatccc a
//
LOCUS       DASUTG010000239         4503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1075539, whole genome shotgun sequence.
ACCESSION   DASUTG010000239 DASUTG010000000
VERSION     DASUTG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075539"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1118)
                     /locus_tag="VF089_05345"
     CDS             complement(93..1118)
                     /locus_tag="VF089_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173418.1"
                     /translation="MGRLSLTLVAASWMLVLSSFAPAIAQDSFYQGKTVRIIVGASAG
                     GGYDTYSRTIARHIGKHIPGNPTFAVENMPGAGFLISANHIYNIAKPDGLTIGHFIGG
                     LFLQQLLGKPGIEFESRKFGFVGAPAQDNYVLGVAKSTGISSMDQWLSSKQIVKLGGV
                     GAGSATDDIPKVLAATIGLPMQLVSGYKGTADVRLAFNSGEVQGVCNAWESFKATWPR
                     EVKSGEIHVVLQAIPKAHPDLPKIPLAISFAKTDEAKKLINALVHSVGPTARPYVLPP
                     GTPKDRVQILARAFMETMKDAEFIADATKAKLDINPLDGAELERNVQTVFELDEALVQ
                     RAKEIMK"
     gene            complement(1159..2184)
                     /locus_tag="VF089_05350"
     CDS             complement(1159..2184)
                     /locus_tag="VF089_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173419.1"
                     /translation="MFKFLGTGLSALILGCYLLAVPAGAAAPYYEGKTIRLIVGFSAG
                     GGFDTYSRVIGRHMGRHIPGNPSIVVENMTGAASLVAANHVYKVAKPDGLTILNFHGN
                     QVINQIIGKPGIEFDARKFEYIGIPTQDNVACAFTKASGITSIDALRNAKTPVKVGGV
                     APGDTTYNTAKLLIAALKLPIQLVAGYKGTAEVRLAAEAGEVAGGCWQWESIKSIWRQ
                     GLDSGNVSVVLQVNPKPHPELPKVANAIDFAPNENSRQLLKYGGHDPAAITRPYALGP
                     GTPKDRVAMLRKAFVDTLKDPEFLADAKKSRLDTDPLTGEEMEKIVAALFKMDANLVN
                     QLKEILQ"
     gene            complement(2240..2470)
                     /locus_tag="VF089_05355"
     CDS             complement(2240..2470)
                     /locus_tag="VF089_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173420.1"
                     /translation="MSKVTPPRAVFVDHPVGRTFGPPQDRSRNEAVLKAALESLPRFT
                     ERNQIHDLQCQWLGDGSRAWEAELRAEMLRDR"
     gene            complement(2561..3031)
                     /locus_tag="VF089_05360"
     CDS             complement(2561..3031)
                     /locus_tag="VF089_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173421.1"
                     /translation="MSFDYLPIMAQAGAPKPPRVLVSPSRQAWSPITKPVSESTVALL
                     SSAALRLEHQEPFVPREDLSYRRIPSDPNAGEVIIDHHSGIGRIPKQDPEIVFPRTAL
                     ASLAQRGIIGAISPFHFSFMGGVRRAQEIEHELAPAIAADLEKMAVDLALLVPY"
     gene            3251..3697
                     /locus_tag="VF089_05365"
     CDS             3251..3697
                     /locus_tag="VF089_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6173422.1"
                     /translation="MDALELLKQDHTKVKELLEKAESAEQGKERTAIFEKIKTELETH
                     ARIEEDVFYPAVQKNEELKDMVLESIEEHKQVKTLLREMDNLSKDSEKFEPKLKVLKE
                     NVEHHAEEEEEGKMFPKVRKLFSATELQELGAELEAAKGSRERKAG"
     gene            3742..4140
                     /locus_tag="VF089_05370"
     CDS             3742..4140
                     /locus_tag="VF089_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173423.1"
                     /translation="METYDVGTQTNGLPSNIDDSEDRVTKNIESYTAAIPSSAYLGIA
                     VGAMALSLFCQITGRGKWGNFIAQWVPTWLIIGVYNKMVKLEGHDRYDRDIMNEPVGD
                     YSCEFCDSKFSLHSDLENHRKHCSVRSPVT"
     gene            complement(4223..>4503)
                     /locus_tag="VF089_05375"
     CDS             complement(4223..>4503)
                     /locus_tag="VF089_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173424.1"
                     /translation="LPTGSEIKSREEIKNTIAKHHDGMLKETKLTNSIEGISTDGNTA
                     IVKGNYHLDGIKMLGFSTGSTGSYEFHGTKRDGRWLIAKAEVTRNDGS"
BASE COUNT         1051 a         1195 c         1235 g         1022 t
ORIGIN      
        1 gccaccatcg atgtgttgcc cgccgattgt gattcttgat ccgccacgga aaatatccta
       61 actacggatc cgctctcatc cgataggaat cgctacttca tgatctcttt tgcccgctgt
      121 accagcgcct catcaagctc aaacacggtt tggacgttgc gctccagttc ggcgccgtca
      181 agcggattga tgtccagttt ggccttggtg gcatcggcga tgaattcggc atctttcatt
      241 gtttccataa acgccctggc cagaatctgc actcgatctt tgggagtacc cggaggcagc
      301 acatagggtc gcgccgtagg accgacgctg tgcaccagcg cattgattaa ttttttggct
      361 tcatcggttt tggcgaaact gatggccaag ggaattttcg gcaagtccgg atgcgcttta
      421 gggatggctt gcaggacaac gtggatttcg ccggacttta cttcccgcgg ccacgtcgcc
      481 ttaaaggact cccatgcgtt acaaaccccc tgcacctcgc cgctgttaaa ggcgaggcga
      541 acgtcggcgg tccctttgta tccgctcacc agctgcatcg gcaggccgat ggtcgcggcc
      601 agcactttcg ggatatcgtc ggttgccgag ccggcgccga cgccgccgag tttgacgatc
      661 tgcttggaag acaaccactg atccatgctg ctaatgcccg tcgatttggc gaccccgagg
      721 acgtaattat cttgagcagg agcgcccaca aagccaaact tgcgcgattc gaactcgatc
      781 cccggtttgc cgaggagttg ctgcaggaac aggccgccga tgaagtggcc gatggtaagc
      841 ccgtccggct tggcgatgtt gtagatgtga ttggctgaga ttagaaaacc cgcccccggc
      901 atattctcca ccgcgaaggt ggggttgccg ggaatatgct tgccgatgtg gcgtgcgatg
      961 gtccgggaat aggtgtcgta gccgcctccg gcagaggcgc cgacgataat cctgaccgtt
     1021 tttccctggt aaaaagagtc ctgcgcgatg gcaggagcaa acgacgaaag caccagcatc
     1081 catgaagccg ctaccaacgt caatgagagt ctgcccataa gacctccgaa tgttgtgccg
     1141 ctattttcag caatgctgct actgcagaat ctctttcaac tggtttacca ggttggcatc
     1201 catcttgaag agcgcggcaa cgatcttttc catttcctca ccggtcagtg gatccgtatc
     1261 gagccgcgac ttcttcgcgt cggcgagaaa ctcagggtcc ttgagcgtat caacgaatgc
     1321 cttgcgtaac atcgcaacac ggtctttcgg agtgccgggc ccgagagcgt aaggtctggt
     1381 tattgcggcg ggatcgtgac cgccatactt gagaagctgc ctgctatttt cgttgggagc
     1441 aaaatcgatc gcattggcta ctttcggcag ctccggatga ggtttgggat tgacttgcaa
     1501 gaccacggag acattgcccg agtcaagtcc ctgacgccag atcgacttga ttgattccca
     1561 ctgccagcaa ccgccggcga cttctccagc ttcggcggcg aggcgaactt cagccgtgcc
     1621 tttgtaaccg gcgaccagtt gaatcggcaa ttttagcgcg gcgatcaaga gcttagcagt
     1681 attgtaagtc gtatctcccg gcgcgacgcc gccgactttc accggggtct tggcgtttct
     1741 cagcgcatcg atcgaggtaa tgccgctagc tttggtaaac gcgcacgcga cattgtcctg
     1801 cgtcggaata ccgatgtact cgaatttacg tgcgtcgaac tctatgcccg gtttgccaat
     1861 aatctggttg atcacttgat tgccgtgaaa attgagaatc gtcagtccgt ccggcttggc
     1921 gaccttgtac acgtgattag ccgcaaccag actggcggcg cccgtcatat tctcgaccac
     1981 gatcgaagga ttgcctggta tatgtctgcc catatgacgt ccgatcacac gggagtaggt
     2041 gtcgaaacca ccgccggcgg agaagcccac gatcaacctg atcgtcttgc cctcgtaata
     2101 gggagccgcg gcaccagccg gaacggccaa taggtagcag ccaagtataa gagctgaaag
     2161 acccgtgccc agaaacttga acataaactc tcctctcgag aattaatctg cggcaattat
     2221 tatgttttaa gctgcgctgt caacggtcac ggagcatttc cgcgcgcagc tccgcctccc
     2281 aagcgcggct cccgtcacct agccactgac attgtagatc gtggatctga ttgcgctcgg
     2341 taaatctcgg caacgactct agcgcggctt tcagaactgc ttcgttgcga ctgcgatctt
     2401 gcggtggacc gaacgtccgc cctacggggt gatcgacaaa gacagctcgc ggcggtgtca
     2461 ctttgctcat aatgtctaga gcagtgccga tcaccagggt cggaataccg cgttcctcaa
     2521 caacgtgagc gatgagaccg acggtctcat ggcaaaacgg tcagtagggc accagcaagg
     2581 cgagatccac ggccatcttt tccaagtcag ctgctatcgc gggggccaat tcgtgttcga
     2641 tttcttgcgc gcggcgaact ccgcccatga aagagaaatg aaacggcgaa attgcgccga
     2701 tgatgccgcg ctgcgccaga gaggcaagtg cggtgcgtgg aaagacgatc tcgggatcct
     2761 gtttgggtat ccggccgatc ccggagtggt ggtcgataat aacttccccg gcattgggat
     2821 cggaggggat acgccgatag cttaagtctt cgcgcggcac aaagggttcc tggtgctcca
     2881 gccgcaaggc tgcgctggac aacagcgcca ccgttgattc actcaccggc ttggttatag
     2941 gggaccacgc ttgccgtgac ggcgaaacta acacacgcgg cggcttaggc gcgccggcct
     3001 gagccatgat gggaagataa tcgaacgaca tagttccctc cgttcgaggc actaattcac
     3061 tccctaatcg tcttcaactc cgtcgagcga gaataaaaat atacgatgac ccagattatc
     3121 acaatgacaa tcagatctga gacagcggcg cgttgcatgt ctcacatctt atacaagtcc
     3181 gcctccgccg ggtctcggcg aagcctggcc ttgctcttgc gtgatactcc tgggcaagga
     3241 ggaacaaata atggatgcac ttgaactgtt aaagcaggat cacacgaaag ttaaggagct
     3301 gctggagaag gcggaaagcg cggaacaagg caaagaacgc accgctatct ttgaaaaaat
     3361 caagaccgaa ttagaaaccc atgcgcggat cgaagaagac gttttctatc ccgcggtgca
     3421 aaaaaacgaa gagctcaaag acatggttct cgaatccatc gaggagcata aacaagttaa
     3481 gactctgctg cgagaaatgg ataacctatc gaaggatagt gagaaattcg agccgaagct
     3541 caaggtcctc aaagaaaacg ttgagcatca tgcggaggaa gaggaggaag gaaagatgtt
     3601 tccgaaggtg cgcaagcttt ttagcgcaac ggagctgcag gaactcggag cggaactgga
     3661 agcggcgaaa ggaagtcgcg aacgcaaggc gggttaagca ttgtggaacg gttagcgtga
     3721 ttggcgaagg agaccttcat aatggaaacg tatgatgtcg ggacccaaac gaacggtttg
     3781 ccgagtaaca ttgacgatag tgaggaccga gtcacaaaga atatcgagtc atatacggcg
     3841 gctataccct cctctgccta cctgggcata gccgttggag caatggcgct ttccttattc
     3901 tgtcaaatta ccggccgggg caaatggggt aatttcatcg cgcaatgggt gcctacgtgg
     3961 ttaatcatcg gggtttataa caaaatggtt aagctcgagg gccacgaccg gtacgatcgc
     4021 gacatcatga atgaaccagt gggtgattac agttgcgagt tctgcgatag taaatttagt
     4081 ttgcactcgg atctcgagaa ccacagaaag cattgtagtg tgcgcagtcc ggtcacctaa
     4141 aatagtcagg cggagcagtc ggctaaatcg ttgagcaacc gattgcttcg ctttgctttt
     4201 tttgtaagtc cgaattaaca gttcacgacc cgtcattccg agtcacttca gctttagcaa
     4261 tcagccagcg accatctctc ttggttccgt gaaattcata gctgcccgtt gaacccgtgc
     4321 taaaccccag catctttatg ccgtccaaat ggtagttgcc tttgactatc gcagtattgc
     4381 cgtcggtaga aatcccctcg atactattcg ttagttttgt ctctttgagc atcccgtcat
     4441 gatgtttggc tatcgtgttt ttgatttcct cacgagattt gatttccgag ccggtaggca
     4501 gac
//
LOCUS       DASUTG010000240         8817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1668515, whole genome shotgun sequence.
ACCESSION   DASUTG010000240 DASUTG010000000
VERSION     DASUTG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668515"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="nusA"
                     /locus_tag="VF089_05380"
     CDS             <1..1241
                     /gene="nusA"
                     /locus_tag="VF089_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX6173425.1"
                     /translation="KIEAQFNPEIGEVELFEAKTVVESVTDAAVEVALNEAKENLDPD
                     AEIGDELLCKLDSTMFGRIAAQAAKQNIVQRVRDAEREIIYNEFKGREGQLVNGIVQR
                     FERRNIIVNLGRTDAILPEKEQVPRERYRQGDRIRAYIVSVEITSRGPQIVLSRTHPG
                     MLIQLFAQEVPEIYEGIVEVKGAAREPGGRAKIAVISNDSDVDPVGACVGMKGTRVQA
                     VVQELRGEKIDIVHWIPDQAEYVCRALAPAKVSKIIIDDDEHSMEVIVPDDQLSLAIG
                     KKGQNVRLAARLTGWRIDVRSEAEAEEETRRARMSIGAIPGINDFAAELLYQAGFKSA
                     EEVSESELEDILDVEGISKEKAESLHKSAKEYVAEKRLKAEEEKAKAAEQAVETAPPG
                     TVSPGESAAGSEPVEGITRD"
     gene            1241..1528
                     /locus_tag="VF089_05385"
     CDS             1241..1528
                     /locus_tag="VF089_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX6173426.1"
                     /translation="MIDIAMARDEHRPQRTCLGCGGRDDQKNLIRITIAADGELITDA
                     RGYGRGGYLHHRHDCWHSFVRRKNTHRAFRVNISKEAKQKLIQVLSEGYRE"
     gene            1532..4315
                     /gene="infB"
                     /locus_tag="VF089_05390"
     CDS             1532..4315
                     /gene="infB"
                     /locus_tag="VF089_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX6173427.1"
                     /translation="MGKMRVHLLAKELEMETKDLVAQLERLGIRGRKAQSSLEDEEVT
                     RVRAALAAQEKPHVHVGEEKVVADRVVTADDQSLGEIHARETIVERRVRANVIRRRTS
                     RVEVPTEAHATEPIQAEPVSAPIPQPRADFEDLAATMPSYEDDSALSAIAPESDVSHH
                     EEAEEAPDEIEEPLSAEETQAQPVPLQDRQPVSAPPAPPPSAQTAAAPAAPPKEAPRG
                     ARVLGRIDLKRTVTIAPAAPAAPRRPIPGSPGVPPAASEAARPARDGEAAKPAAGEAE
                     KPKAGRHKKRVVKKQDVLEFREKELRSGRIPKKKRALPGKEQRKTEITVPRASKRVIR
                     ISEVITVGDLAREMGIKAGDVIKKLMSLGMMASINQVLDVDTATLVAADFDYQVENVA
                     FDAESMLEVEQVEEESMLVSRPPVVTIMGHVDHGKTSLLDAIRSTNVTAQEHGGITQH
                     IGAYHVQVDGRSVTFLDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINH
                     ARAAEVPIIVAVNKIDKPDADLERVKRGLSDHGLVSEDWGGDTVFAPVSAKTQEGIPH
                     LLEMLLLQADILELRANPDKLARGTIVEAKLDRGRGPVATVLVQEGTLRVGDAFVCGV
                     FHGKVRAMINDLGQKVEIAPPSSPVEILGLQGVPQAGDSFIAVADESKARQVAEHRHT
                     KQRETELVKSSKVSLEELYDQIKTGDVKELRVVVKADVQGSVEALTDALTRMSTAEVK
                     LRVLHGSVGGITESDILLAAASNAIVIGFNVRPESKGQALAAQEGVDVRLYTVIYEAV
                     ADVRAAMEGMLEPTFREQTQGRVEIRQIFNIQGVGTIAGCYVTEGKIQRGNLVRILRD
                     HVVVHEGKLASLKRFKDDVREVSAGYECGLSIEGFHDIKQGDVVEAYERTAVVRRIAP
                     VQSGREASHYAGR"
     gene            4369..4641
                     /locus_tag="VF089_05395"
     CDS             4369..4641
                     /locus_tag="VF089_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6173428.1"
                     /translation="MKVNLLLPENHSLKGKRGVLKRIQARVANRFNVSVTECGDQDLW
                     QSAVLGFSLIGTHKELIEKTLNKIVDFIDEIGLAQVGDSDIEFFYC"
     gene            4654..4998
                     /gene="rbfA"
                     /locus_tag="VF089_05400"
     CDS             4654..4998
                     /gene="rbfA"
                     /locus_tag="VF089_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6173429.1"
                     /translation="MDYQRSERVGELLTEVIADVLRKDIRDPRVTAVTITSAKLTKDL
                     RQARIYFTILGGASENKDSVLAGLKSALGFIRARIGKQLKLRFVPAIEFFYDESVDEA
                     ERIEKLLRQVKQ"
     gene            5058..5957
                     /gene="truB"
                     /locus_tag="VF089_05405"
     CDS             5058..5957
                     /gene="truB"
                     /locus_tag="VF089_05405"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6173430.1"
                     /translation="MIKSGLILVDKPEGPSSAHVVHRAKVSLGAKKVGHLGTLDPFAS
                     GLLLLGINEGTKISDIFLRAPKSYRGVVTLGVATDSQDSTGVVIKTRPVPPLADPELR
                     LLEQRFSGILQQTPPMFSALKRDGIRLYKLAREGKEVPRQPRTIRIDAIRLRQINRTE
                     ISIDVTCSRGTYVRTLASDLGEALGCGAHLKSLRRIACGHLRIERAVTLDDLERSATR
                     AESAMVSLSDALSHLRGIIWDHRWLARLRLGQQECLGQIGKPLEEQELVRLLDARGNL
                     VALIGWNEENPGAGWQLRRVFKE"
     gene            complement(6090..6734)
                     /locus_tag="VF089_05410"
     CDS             complement(6090..6734)
                     /locus_tag="VF089_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6173431.1"
                     /translation="MQKKILLCAFGITVDGHHEMIDFYPAASESAACWEAFLGDLYRR
                     GLKASPCQLIATDGGTGLHQALQIVYPKTLLQRCWAHKARNVLDKVKKKDQPAVKKAL
                     HRISHASNRREAAQAYWRFSSRWRKAYPRAVLCLKKDFDQLLSFFAIKNSNLWSRLRT
                     TNLIERAFREVRRRTRPMGVMAHTQSLQRIVFAVFHHLNTNWSLNPVKDFTHNS"
     gene            complement(6755..7420)
                     /locus_tag="VF089_05415"
     CDS             complement(6755..7420)
                     /locus_tag="VF089_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6173432.1"
                     /translation="MTFTLGREERIERWLREKPKSFLDWEKIEGVLTPELCNQHKQEE
                     GTPMLLDQATSRNVSRALSEINGFDWEGDFKPMALQVLKDLVQKQLEEEMAEYLGLSR
                     YEHALDRPDYRNGYYVRHLLTEMGDLELLVPRSRKGGFPTKLFERYARRCRSVDRVLL
                     ACFCLGLSTRKAASVLAPVLGEKVSASTISRIARDLDQEVSRYHDRSLPDRYRYLFFD
                     GVV"
     gene            7643..>8817
                     /locus_tag="VF089_05420"
     CDS             7643..>8817
                     /locus_tag="VF089_05420"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX6173433.1"
                     /translation="MINPTAKVTANPHIQKWVKDMAAMCQPDNVYWCDGSESEREQLT
                     EVAVEIGDLMPLNQNKLPGSYLHRSALNDVARTENLTFVCTETEEEAGPNNNWMAPAK
                     SYERLGKIFTGAMRGRTMYVIPFLMGPEGSAFSKVGVQLTDSVYVVLNMRIMTRMGKV
                     ALDHLGSSNDFTRCLHSKADLDMDRRFICHYPQDNTIWSVGSGYGGNALLAKKCLALR
                     IASKLGQKEGWLAEHMLIVGVESPEGETHYICGAFPSACGKTNLAMLVPPASMKGWKI
                     HTIGDDISWLRVGSDGRLWAINPEAGFFGVAPGTGSKTNPNAMKTVQRDTIFTNVALR
                     SDGTVWWEGHDDPVPANAIDWRGKAWDPKSGHPAAHPNSRFTVSLKQCPTYSPEWEKP
                     "
BASE COUNT         2074 a         2294 c         2558 g         1891 t
ORIGIN      
        1 ggaaaatcga agcgcagttt aatccggaga ttggcgaagt ggagcttttc gaagccaaga
       61 cggtggtgga gagtgttacc gatgccgcgg ttgaggtggc cctgaatgag gccaaagaaa
      121 acttggatcc ggatgctgaa atcggcgatg agcttttgtg taagttggat tccaccatgt
      181 tcggccgcat tgccgctcag gcagcgaagc aaaatatcgt gcaacgtgtg cgcgacgccg
      241 agcgcgaaat tatttacaat gagtttaagg gtcgtgaggg gcagctcgtt aacggcatcg
      301 tgcaacgctt cgagcggcgt aacataatcg ttaatctggg gcgcaccgac gcgatactgc
      361 ctgaaaagga acaggttccg cgagagcgat atcgacaggg tgaccggatc cgcgcctata
      421 tcgtcagtgt ggagattacc agccgcggcc cacaaattgt cttgtcgcgc acacatcccg
      481 gcatgctgat acaactcttc gctcaggaag tgccggagat ttacgagggt attgtcgaag
      541 tcaaaggcgc cgcgcgtgaa ccgggcggca gggcaaagat agcggttata tccaacgatt
      601 cggacgttga tcccgtcggt gcctgcgtcg gtatgaaagg cactcgtgtt caggcagttg
      661 ttcaggagct gcgcggcgaa aaaatcgaca ttgtgcattg gataccggac caagcggaat
      721 atgtttgccg agccttggcg ccggccaagg tatccaagat catcatcgat gatgacgagc
      781 acagcatgga agtgattgtc ccggacgatc agctgtcgtt ggccatcggc aagaaggggc
      841 aaaatgtccg gttggccgcg cgtctgaccg gctggcgcat cgatgtccgc agcgaagcgg
      901 aggccgaaga agagacgcgc cgcgcgcgca tgtcgatcgg cgcaatccct ggcatcaacg
      961 attttgccgc ggagctactt taccaggccg ggtttaaatc ggcggaggaa gtttctgaaa
     1021 gcgaactcga agatattctc gatgtcgaag gaatcagcaa agaaaaggct gagtcgctgc
     1081 acaaatcggc taaggaatac gtcgctgaga aacgcttgaa agcagaggaa gaaaaggcca
     1141 aggccgccga acaagcggtt gaaacggcgc cgcctggaac tgtgtcgccc ggcgaatctg
     1201 cggccggttc tgaaccggtg gaaggaatta cgagagattg atgatcgaca tcgcaatggc
     1261 acgggatgaa catcgtccgc agcgaacgtg tttgggctgt ggtggtaggg acgatcagaa
     1321 aaatctcatt cgtatcacca ttgccgcaga cggcgagttg attaccgatg caagaggata
     1381 cgggcgtggc ggttatcttc atcaccggca cgactgctgg cactcgttcg tccgccgcaa
     1441 gaacacacac cgagcatttc gtgtgaacat tagcaaagaa gcaaaacaaa agctcattca
     1501 ggtcttgagc gagggatatc gggagtaaca gatgggaaaa atgcgagtcc atctattggc
     1561 aaaagagttg gagatggaga ctaaggatct cgtcgcgcag cttgagcgtt tgggaatcag
     1621 gggacgcaag gcccaaagct ccctcgagga tgaggaagtc acgcgtgtgc gcgctgctct
     1681 agcggcgcag gaaaaaccgc acgtgcatgt cggcgaagag aaggtcgtgg ccgaccgagt
     1741 cgtgacggcc gatgaccaaa gcttaggcga gatccatgcg cgcgagacca tcgttgagcg
     1801 gcgggttcgc gctaacgtga ttcgccgacg aacgagtcga gttgaggttc cgacggaagc
     1861 ccatgcgacc gaaccaatcc aggcggaacc cgtttcagcg cccatcccac aaccgcgtgc
     1921 cgacttcgaa gatttagccg ccactatgcc ttcctatgaa gatgattcgg ctttaagcgc
     1981 gattgcgccc gagagcgatg tttcacatca tgaggaagcg gaagaggcgc cggatgaaat
     2041 cgaggagccg ctttcagcgg aagaaacgca agctcagcca gttccgctac aagatcgaca
     2101 gccagtttca gcccctcctg ctccgccgcc ctctgcccaa acagcggcag cgccagctgc
     2161 gccgccgaag gaagctccac gcggcgcgcg agtgctcgga cgcatcgatc taaaacgtac
     2221 tgtcacgatc gctccggcgg caccggctgc gccgcggcgc ccgataccgg ggtcacccgg
     2281 agttccgcca gccgccagcg aagcagcacg gccggcgcgc gacggcgaag cagccaaacc
     2341 tgctgccgga gaagccgaga agcctaaagc cggtcggcac aaaaaacggg tcgtcaaaaa
     2401 acaggatgtt ttggaatttc gcgagaagga gctgcgctcc gggcgcatcc caaagaagaa
     2461 gcgcgctctg cccggcaaag aacagagaaa aaccgaaatc accgtgcccc gcgccagtaa
     2521 acgcgtcatc cgcatctcag aagttatcac cgtgggagat ttggcgcgcg agatgggcat
     2581 caaggccggg gatgtgatca agaagttgat gagccttggc atgatggcca gcataaacca
     2641 ggttctggat gtcgacaccg caacgttggt ggctgccgat ttcgactatc aggtggagaa
     2701 tgttgctttc gatgccgaga gcatgttgga agtcgagcag gtggaagaag agtcgatgct
     2761 ggtttcgcgc cctcctgtag taaccattat gggtcatgtc gatcacggca aaacctcgct
     2821 cctcgatgcc attcgcagca ccaatgtcac ggcccaggaa catggcggca ttactcagca
     2881 tattggcgcc tatcatgttc aggttgatgg ccgcagtgtc acatttctgg atacgccggg
     2941 ccacgaagct tttacggcga tgcgcgcccg gggtgctaaa gtcaccgata tagttgttct
     3001 cgtcgtcgct gctgatgacg gtgtcatgcc gcaaacagtt gaggcgatca atcacgctcg
     3061 tgcagcggag gtgccgatta tcgttgccgt caacaagatc gataagcccg acgcagatct
     3121 ggaacgtgtg aagcggggac tttctgatca tggtctggtc tcggaagact ggggtggcga
     3181 taccgtgttt gctccggtct cggcaaaaac tcaggaggga attccgcacc tgttggagat
     3241 gttactgtta caagcggata ttctcgagct ccgggccaat cccgacaagc tagcgcgtgg
     3301 gacaatagtt gaggcaaaac tggatcgcgg ccgtgggccg gttgccaccg tcctggttca
     3361 ggaaggaacg ctgcgcgtcg gcgatgcgtt cgtttgcggc gtgttccacg gaaaagtgcg
     3421 cgcaatgatc aatgatctcg gtcagaaggt tgagatcgcc ccgccctctt ccccggtcga
     3481 aatcctcggc ttgcagggtg tgccacaggc gggcgactcc ttcatcgcgg tggccgacga
     3541 gtccaaagca aggcaggttg ccgagcatcg tcacaccaag caacgggaaa ccgagctggt
     3601 taaatcgagc aaagtgtcgc tggaagagct ctacgatcag atcaagaccg gtgatgtgaa
     3661 ggagctacgg gtcgtggtca aggccgatgt gcaaggttcc gttgaggcgc ttaccgatgc
     3721 gcttacgcgc atgtccacag ctgaagtaaa gttgcgggtc cttcatggtt cggttggcgg
     3781 catcaccgag agcgatattc tgttggccgc ggcttccaac gcaatcgtga tcggcttcaa
     3841 tgtccgtccc gaatctaaag gccaagcctt ggcggcacag gaaggtgttg acgtgcgcct
     3901 ctatacggtt atttacgaag ccgtcgctga tgtccgggca gcgatggaag gcatgttgga
     3961 accgaccttc cgcgagcaaa cccaaggtcg agtcgagatt cggcagattt tcaatatcca
     4021 aggggtcggc acaatcgccg gctgctatgt caccgaaggt aagattcagc gcggtaactt
     4081 agtgcgcatc ttgagagacc atgtggtcgt gcacgagggc aaactcgcca gcttgaagcg
     4141 ctttaaagat gatgttcggg aagtgtcggc cggctacgaa tgcggtttgt cgatcgaagg
     4201 gtttcacgac atcaagcaag gtgacgtggt tgaggcttac gagcgaaccg cagtggtgcg
     4261 gcgcatcgct ccggtccaaa gtggtcgtga agcctcgcat tatgccggga gatagcgatc
     4321 ggccgtaagc attctcgaga ctcgagtccg agtgcggtcg tcggggtcct gaaggtcaat
     4381 cttctactgc cggagaatca ctcgttgaaa ggcaaacgcg gcgtgcttaa gcgtattcag
     4441 gcgcgagttg ccaaccgctt taacgtgtca gtgaccgaat gtggtgacca ggatctatgg
     4501 caaagtgccg tgctcggctt tagcttgatc ggcacccata aagaacttat cgagaagact
     4561 cttaacaaaa tcgttgactt catcgatgag attggattag cgcaggtcgg cgattccgat
     4621 atcgagttct tctattgtta attctgtttg ctcatggatt atcaacgttc agaacgcgtg
     4681 ggcgaattgc tcaccgaagt tatcgcggac gtattacgaa aagatattcg cgacccacgc
     4741 gttactgccg tgacgattac ctctgcaaag ttaaccaagg atttgcggca ggcgcggatt
     4801 tatttcacga ttctaggcgg cgcttctgag aacaaggact cggtgctggc gggattgaag
     4861 agcgccctgg gatttatccg tgccagaatc ggcaagcagc tcaagcttcg cttcgttccg
     4921 gctatcgaat ttttctacga cgaatctgtc gacgaggcag agcgtatcga gaagcttctc
     4981 aggcaagtaa agcagtaacg ctagtctcgt ttaaagcccc ggctctcatc ttcagtttct
     5041 aaaacgtgca aaattccatg ataaaaagcg gtcttatttt ggttgataag ccggaggggc
     5101 cgtcatcagc gcacgtcgtg catcgggcaa aggtatctct cggtgcaaaa aaggtaggcc
     5161 atctcggtac cctcgatcca tttgcctcgg gattactcct cttgggaata aacgaaggga
     5221 caaagatctc ggacattttt ttgcgtgcgc cgaaaagcta ccgcggtgtt gtaacgcttg
     5281 gagtggcaac cgactcgcag gattccactg gagttgtgat aaagacgcgc ccggttccgc
     5341 cgctcgccga ccctgagctg cgtcttctgg agcaacggtt cagcgggatt ctgcagcaga
     5401 caccgccgat gttctccgcg ctaaaaaggg acgggatccg gctatacaag ctggcgcggg
     5461 agggcaaaga agtgccgcgg cagccgagaa caatccggat cgatgctatt cgattacgtc
     5521 agatcaatcg tacggaaatt agcatcgacg ttacctgctc acgagggact tacgttcgga
     5581 ccttggcttc agatttgggt gaagctctcg gttgtggcgc gcatttgaag agcttgcggc
     5641 gaatcgcttg cggtcatctc agaatagagc gggctgtaac cctcgatgac ctcgagcgat
     5701 ctgcgacacg agcagagagc gcgatggttt ccttgagcga tgcgcttagc catctaagag
     5761 ggatcatttg ggatcatcgc tggcttgccc gtcttcgtct cggtcagcag gaatgcttgg
     5821 gtcagatcgg caaaccgctt gaagaacagg agctagtacg gttactggat gctcgcggga
     5881 acctggtcgc cctgatcggc tggaacgagg aaaatcctgg cgctggttgg caattgcggc
     5941 gcgtgttcaa agaatagcat gtgacgattg taccgcggag gcggcgctgg gctccatgca
     6001 caatcaatcc gtctccagtc ctgctctccc cttgttcatt taaatagaga gatgcgagta
     6061 tcgcgccgta atcacgcgcg ccggtagcgt caagagttgt gtgtaaaatc tttgacgggg
     6121 tttagactcc agttagtatt caagtgatga aacacagcga agacgattct ttgtaagctc
     6181 tgggtatgag ccatcacccc cataggccga gttcttcgcc tgacttctcg aaaagctcgt
     6241 tcgatcaagt tcgtggttct taagcggctc cacagattag agttcttgat ggcaaaaaag
     6301 ctcagcagct gatcgaagtc tttctttaag caaagaactg ctctgggata ggcttttctc
     6361 cagcgtgagg aaaatcgcca gtaagcctgg gccgcctctc ggcgattgga cgcatggctg
     6421 atccggtgta aagccttctt gaccgccggc tggtctttct ttttaacttt atccaagacg
     6481 tttctggctt tatgcgccca gcagcgctgt aggagagtct tgggatagac aatctgtagg
     6541 gcctgatgca gtcccgtgcc gccatcggta gcgatcaact ggcacgggct tgctttgagt
     6601 cctcttctgt aaagatcgcc taaaaaagct tcccagcagg ccgccgactc actggcggcc
     6661 gggtaaaagt cgatcatctc atgatggccg tccacggtga tgccgaaggc gcaaaggagg
     6721 attttctttt gcaccttaag cgctcctttg ctctttacac cacaccatcg aagaacagat
     6781 agcggtagcg atccgggagc gagcggtcgt gatagcgact gacctcctga tccagatccc
     6841 gtgcaatgcg tgagatggtc gaggccgaga ccttctcgcc caaaaccgga gccagcactg
     6901 aggcagcttt gcgggtcgac agccccaaac aaaaacacgc caatagaacc ctatccactg
     6961 agcggcagcg ccgggcatag cgctcaaaga gcttggtcgg aaaaccccct ttgcggctgc
     7021 gcggcactaa cagctctaaa tcacccatct cggtcagcag atgcctgacg taatacccat
     7081 tgcggtagtc cggccgatcc aatgcatgct cgtaacgaga gagccctaag tactccgcca
     7141 tctcttcttc gagctgcttc tgcaccaggt ctttgagcac ctggagcgcc atcggtttaa
     7201 agtctccctc ccaatcaaaa ccgttgatct cactaagagc tcgcgataca ttccttgacg
     7261 ttgcttgatc gagcagcatg ggggtgccct cctcttgttt gtgttggttg cacaactctg
     7321 gagttagcac cccttctatt ttttcccaat ccaaaaatga cttaggcttt tcccttaacc
     7381 acctctcgat cctctcttct cgacccaatg tgaaagtcat tttctcccat cctctctccc
     7441 ccaaatcgaa tttacacaca gatccataca tcaccacgcg cgccaactca gttgacattg
     7501 ccgcttttga tcggatagtt ttatcactga agcaatgctt gccggtctgg ctcttgattg
     7561 gtcgtcgtct agccggacca gaattccgca ggtcctgaaa tgactcgaac ttcacgtcgt
     7621 ttgaaaggga gaattgtgtc gcatgattaa tccaacagcc aaggtaacgg caaatcctca
     7681 tatccaaaaa tgggtgaagg acatggcagc gatgtgccag cctgacaatg tctattggtg
     7741 tgacggttcg gaatcggaac gcgagcaact cacggaggtc gccgttgaaa tcggcgattt
     7801 gatgccgctg aaccaaaaca aattgccggg ttcgtatttg caccggagcg cgctcaacga
     7861 tgtcgcccgt accgaaaatc taacttttgt ctgtactgaa acggaagagg aagccgggcc
     7921 caacaacaac tggatggcgc cggctaagtc ttatgaacgc ctagggaaaa tttttaccgg
     7981 cgcgatgcgc ggtcgaacga tgtacgtcat ccctttcctc atgggaccag aaggttccgc
     8041 cttcagtaag gttggggtgc aactcacgga tagcgtctat gtggtgctga atatgcgcat
     8101 catgacgcga atgggcaaag tggctctaga ccatctaggc agctcaaatg attttactcg
     8161 ctgtctgcat tccaaagctg acctcgacat ggaccggcgc tttatctgcc actatccgca
     8221 agataacacg atctggagtg tcggctctgg gtatggtggc aacgccttgc ttgcgaagaa
     8281 gtgtttggcg ctgcgaatcg ccagtaaact cggtcaaaaa gaaggctggc tggccgagca
     8341 catgctgatc gttggcgtcg agagcccgga gggcgagact cattacatct gcggcgcttt
     8401 cccgagtgcg tgcggtaaaa ccaacctcgc catgttggtt ccgccggcga gcatgaaggg
     8461 gtggaaaata cacaccatcg gcgatgatat ctcctggctc agggttggtt cggacggtag
     8521 attgtgggcg attaatcctg aagcgggctt cttcggcgta gcaccgggta ccggatccaa
     8581 aaccaatccc aatgcgatga aaacggtgca aagggacacg atattcacaa atgtggcgct
     8641 tcgatccgat ggcacagtgt ggtgggaagg acacgatgat cccgtaccgg ccaatgcaat
     8701 tgactggcgc ggcaaggcct gggatccgaa gagcggtcat ccagcggcgc acccgaactc
     8761 ccgattcaca gtgtcactta agcagtgtcc tacgtactct cccgaatggg aaaaacc
//
LOCUS       DASUTG010000241         9420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1817657, whole genome shotgun sequence.
ACCESSION   DASUTG010000241 DASUTG010000000
VERSION     DASUTG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817657"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..2732)
                     /locus_tag="VF089_05425"
     CDS             complement(513..2732)
                     /locus_tag="VF089_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX6173434.1"
                     /translation="MVKQPEKITVQGTVQKITYHHPDTNYTVLRLDVENAPGVTVVGG
                     VYPVAEGEEIKVFGFWKTHPRYGQQFQVNHWEKVDPATLEGIEKYLGSGMIKGIGPTY
                     ARRLVDAFGLETLRILSQEPHRVLEVEGIGAGRARAIMQAWEAQRGMQDIMVFLQGHG
                     IGAALALKIYRFYGLDTIRRVKDNPYALAREIYGVGFVLADRIAARLGISGDFPLRVQ
                     AGVLHVLKEFVDQGHCFVLFSALIRNAAALLRVGEDVTENAVQQLAVSGDVILERTTS
                     DERAHVYLCELYRAEQRVAEALHRLLSSPSVTLGGGRKPPGSTAEPRQLLGLSDLELI
                     DPSFAILEEEQQEAVRQALQQKVLVITGGPGTGKTTLLNSLLALLRRAKFSFVLAAPT
                     GRAAKRMAESAGEEAMTIHRLLEYNPHEGGFQRSEDRPLEADVVIIDEASMVDLALMD
                     NLLSAIDRHSHLVLIGDVDQLPSVGPGSVLRDLIDSGMIPVVMLRRIFRQDRHSLIVA
                     NAHRILQGQSLLTPNDSLERDFILLARESEEEILRSLKVLVKEELPQTLNLAADEVAH
                     AIQLLTPMHRGMLGTIQLNREMQSLLNPIGDSLQRGDSLLRTKDKVMQLRNNYDKGIY
                     NGDLGRITGIDPKEGKVQVDFIDKLVEYNDDELDEISLAYATSIHKSQGSEYPVVVIP
                     LHMSHYMMLHRSILYTAVTRGKRRVVLVGSRRALAMAIHNVRLERRYTGLKEKLAKL"
     gene            3051..4373
                     /locus_tag="VF089_05430"
     CDS             3051..4373
                     /locus_tag="VF089_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6173435.1"
                     /translation="MIVENLLFEAAVIFLLILANGFFAASEIAMIAIRKSRIDTMIEK
                     GVKRALIIGRLKKDPDRFLATVQIGVTVVSSLASAIGGAAAISFVKPAIEASAIPALA
                     PWADAIAIAVVVIPISYLSLVFGELVPKSLALRFSEQIACMAARPIDLLSRLTSLLVK
                     FLTGSSNAVLRLFGGSEVEGASFISVEEVKSLIREGAAKGIFNETEKELIHGVFELAD
                     TPVKAVMVPRTEIHALEIHSSPSEAAASFIESGFSRIPIYDGELDHVVGVLYNKDFFK
                     TLQQKTEFRIKDHLHPAFFVPSTLPISELLKQLQRRRLAMAMVVNEFGEVEGLATLED
                     LLEEIVGEIRDEYDREERGPVDRLPDGSMVIQGSALLKDLKTDFDLPFEESPDYHTLA
                     GFLLAKLKRIPRGGEWVEENGYKLTIVDMEGRRIVKIKLEQTNPQKQL"
     gene            complement(4380..4796)
                     /locus_tag="VF089_05435"
     CDS             complement(4380..4796)
                     /locus_tag="VF089_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173436.1"
                     /translation="MSGFKISELDHIVLNVSDIERSLQFYTGVLGLKAERLDEFRTGK
                     AGFPSVRINTDTIIDLFPVREQQSRPSDGEKTAGNLNHFCMVIGKEDFAGIVDYLKEN
                     SIIIREGPVSRWGARGRATSVYFLDPDGNEIEIRCY"
     gene            complement(4783..5886)
                     /gene="hisC"
                     /locus_tag="VF089_05440"
     CDS             complement(4783..5886)
                     /gene="hisC"
                     /locus_tag="VF089_05440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6173437.1"
                     /translation="MSRYFRPNIASMAGYVPGEQPGDEKLTKLNTNENPYPPSPGVLA
                     ALREATNQSLRLYPEPLSDSLRNIAAEVYGVGPQNILAGNGSDEILSIVLRCFVGSGD
                     RVAFPVPTYSLYETLVKIQDGQQVCVDYHADYLIPPELHSAKAAVTFLCNPNSPSGTL
                     VSLTEIERLARAVAGVIVVDEAYIDFAEEPDSSAIPLIQSVPNLIVLRSFSKSFSLAG
                     LRIGLAFAPIELIAGMMKVKDSYNVNRLSLVAATAALHDLPWMQRNVRRIQQTRRQLI
                     EGLVKLGFYVYPSHANFVMAQRKGQEQTALYQALRERKIYVRYFDTPELRDSLRITVG
                     TPEDVERLLQEIAAIQKESGDPSQTEETNHERI"
     gene            6324..7481
                     /locus_tag="VF089_05445"
     CDS             6324..7481
                     /locus_tag="VF089_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173438.1"
                     /translation="MHNRNQCEIDEVAALQQAIGAKELLLREREAAFEALEQRLNSTI
                     EQLSADLNEKQTLIGQNEMRSRQAKADVIEIVEQKTRLEMLQKQTERLLSAQAEQIRA
                     GVRAEIQYLERQLMDKESELQKCQACTIDLQHRLAEMQLLSKSRAVQIEDLKAETVRL
                     SQDISRHESVKLGTGSYADHELRYGREPEAWAGEISAKLDTNSHDAEIAEAPPRNAIS
                     TLRQEAEEKHLLLASRNEELVRVKAELDLLQGRISELNASNERIQESAETECAKMRAE
                     FQAQLAFLQAELSQRDWALEEREAAQKVLEQGLRAKIGQLELQLAQIKSSAEGPAQEF
                     VLGENWESEARLDHTWKLQERLDGNGAVGAQTPSDRSGKWRGSGRWKRRWR"
     gene            7545..8261
                     /locus_tag="VF089_05450"
     CDS             7545..8261
                     /locus_tag="VF089_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX6173439.1"
                     /translation="MTERVTTGIPGLDQLLEGGFPKGRSILVTGEPGTGKTIFALQFL
                     VEGLRRGEKGIYVAADEGPTDIIEQAASLGWDLEKYIAAKELAILNAGTYLSSSPGTG
                     KERQIDIHKAIGDLAGFVNRLGAKRLVLDPAGPFVLLRDTATRIQDQTRLLIKLLRTS
                     MQTTNILTSYGVPRTGERTLHGIEEYLVAGAIILEIIWRDGKLDRSMVIEKMRCTDVK
                     PAQHAFDINKVQGFVLNPLP"
     gene            8285..9073
                     /locus_tag="VF089_05455"
     CDS             8285..9073
                     /locus_tag="VF089_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX6173440.1"
                     /translation="MDPQRVSTGVSGLDSQLIGGFIKGRSYVILGDAGTGKTTACLQF
                     LLSGLKQGEKGLYITVDERPTEIIESAASLGWDFESYTHDKSLVMLDASPYFSGRAGT
                     SPDKSVDLQKIVSDLAGYAKRIDATRLVIDPVTPMIFSDSPSRVQDQARSFIHLVHSQ
                     LNTTNLFSAQYSSRAPDPSFGIEEFLVSGVLHLKTTPVDGGFGRKLTIKKMRGTPIEP
                     SEYWFAITAGEGIVLTPERERPAAVQESGHTTLLEYFEPPAKAK"
     gene            9251..>9420
                     /locus_tag="VF089_05460"
     CDS             9251..>9420
                     /locus_tag="VF089_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX6173441.1"
                     /translation="MRLDKLTTKSQEALQHAQALAEKRNHQAIDVEHLLFALLGQKEG
                     VVLSLLQKLSVPL"
BASE COUNT         2195 a         2591 c         2533 g         2101 t
ORIGIN      
        1 accaggcaac gttatgggca tgcctcaagc ggctgggcat ctctcactcg gacaacaggc
       61 ttgggcgcct ttttgattaa cgctccggag tttaacaatc cgaattcctc cgaaggaggt
      121 gaaggaacaa gccttccgac cgcagccgat gggtctctgc caccaaggcc gcccgcgcca
      181 gcacaaggtg cttgttacaa cgatttggcc tggcagcacc tgcacgtcta cgtctgcagc
      241 ctccaggctt gctccttcac gccgggtctt cacactcaaa ctgttttctt cgagagacct
      301 gctcgaaggt ggggtaaggc tggaaaggtg aaggcatccg agtcatgaaa gtccaatgac
      361 ttctttggtg ctcggccaga cttttgaagt cgtttccggc ggtcgctcca ccaccggccg
      421 tcaataaacg cctcaaggca ccaacccatt gccttcgcct tggaagcctt gcgccgacgg
      481 atactagagc gtgaatggtc tggactgagg ctttatagtt tcgccagctt ttctttcaag
      541 cccgtatagc gccgttccaa ccgcacgttg tgaattgcca tggccagagc gcgccggcta
      601 ccgacgagta ccactcttct cttgcctcga gtgaccgcag tgtagagaat acttcgatgc
      661 agcatcatat agtgggacat gtgaagcggg atgaccacca ccggatattc gctgccctgg
      721 ctcttgtgaa tcgatgtggc atacgccagg cttatttcgt ccagctcatc gtcattgtac
      781 tcgactaact tgtcgataaa atcgacctga accttgcctt ccttaggatc aatgccggta
      841 atccggccaa gatcgccatt gtagatacct ttgtcatagt tgttgcgcag ctgcatgacc
      901 ttatccttag tgcgcagcaa ggagtcgccg cgctgcagcg aatcgccgat aggattgagt
      961 aaactctgca tctcgcgatt gagttgaatc gtcccgagca tgcccctgtg catcggcgtt
     1021 aagagctgaa tcgcatgtgc cacctcgtcg gctgcaaggt tcaaagtttg cggtaactcc
     1081 tctttgacga gcaccttgag cgaccgaaga atttcttctt cggattcccg tgccagaaga
     1141 atgaagtcgc gctccaagct atcgttcggc gtcagtaacg actgcccctg aagaatacga
     1201 tgagcattgg caacaatcag gctatggcga tcttgccgga agatgcgccg caacatgacg
     1261 accggtatca ttccggaatc gatcagatcg cgcagcacgc tgcccggtcc caccgatgga
     1321 agctgatcga cgtcgccgat gagaacgaga tgactatggc ggtcgatggc actgagcaaa
     1381 ttatccatga gggctagatc gaccatcgag gcctcgtcga tgatcaccac gtcagcttcc
     1441 agcggtcgat cctcgctccg ttgaaagccg ccttcatgcg gattgtattc cagcagccgg
     1501 tgaatcgtca ttgcttcctc gcccgcgctt tcagccatgc gcttggccgc tcgtccggtc
     1561 ggcgcggcaa gaacaaagga aaatttcgcg cgccgcaaga gagccaataa tgaattgagc
     1621 aaagtggttt taccggttcc aggtcccccg gtgatgacca gaaccttttg ctgcagcgcc
     1681 tggcgcacag cttcttgttg ttcctcttcg aggatagcaa aggatggatc gatgagttcc
     1741 aaatcgctca gccctagcaa ctgccgtggt tcggctgttg atcccggggg ctttcttcca
     1801 ccgccgagcg tgacagaagg gctcgacagc agccggtgca gcgcttcagc gactcgctgc
     1861 tcggcacgat aaagctcgca cagatagaca tgcgcccgct cgtcgctggt ggtccgttca
     1921 aggattacgt cgccgctgac agccagctgc tgcaccgcat tctcggtcac atcttcgcca
     1981 acgcgtaaca gcgccgcggc gttcctgatg agagccgaga aaagcacgaa acaatgcccc
     2041 tgatcgacaa actctttgag gacatgaagc acgcccgcct gcacacgaag cggaaaatca
     2101 ccgctgattc cgagtctcgc cgcgatgcgg tcggctagga caaacccgac accatagatc
     2161 tcgcgggcca gcgcataggg attgtccttg acccgtcgaa tggtatccaa tccgtagaat
     2221 cggtaaattt tcagcgccaa tgccgcgccg atgccgtgtc cttgcaagaa caccatgata
     2281 tcctgcattc cccgctgcgc ctcccaagcc tgcatgatcg cgcgggcacg ccccgcgccg
     2341 atgccttcga cttcgagaac acgatgcggc tcctgggaaa gaatcctcaa agtttccaat
     2401 ccgaaagcat cgaccagccg gcgggcgtag gtcggcccga tgcctttgat catgcctgag
     2461 ccgaggtatt tttcgatgcc ctccaaagtc gcgggatcaa ccttttccca atgatttact
     2521 tgaaattgct ggccgtagcg tggatgcgtt ttccagaaac caaagacttt gatctcctcg
     2581 ccttccgcaa cgggatagac tccgccgacc accgtaacgc ccggcgcatt ttcgacatcc
     2641 agccgcaaaa ccgtatagtt agtgtcggga tgatggtagg ttatcttctg tacggtgccc
     2701 tgcaccgtga ttttctctgg ctgttttacc acgacgtcta attcctagcc tgcaaggatc
     2761 aagcgatcat ttccgtgaga gccttaggtt tcgaccgggg cgcttctcgg tccttctgca
     2821 gcgatgacgc cgcagccgcc gagttgaacc gacgagtcct tttgtagttt ggctataaat
     2881 aacaagtgct tgcgttttcc ggagcggatg cttacattcg aatgtagcag atatgcgccg
     2941 cctcccgcac ctgacctatc cattccagaa cgttgcctcc tggtgatact cacggccgaa
     3001 accgtgtttt tccgtcgtct tcgagctgaa gacacggagg agagattttg atgatcgtcg
     3061 aaaatcttct atttgaagca gccgtcattt ttcttttgat tctggccaac ggattcttcg
     3121 ctgcttcgga aatcgcgatg atcgcgatac gtaaaagccg tatcgacaca atgatcgaaa
     3181 agggtgtcaa acgcgctttg ataatcggcc gccttaagaa ggatccagac cgatttttgg
     3241 ccacggtaca gatcggggtg acggttgtca gctcgctggc gtccgccatc ggcggcgctg
     3301 ccgcaatctc ctttgttaaa ccggctattg aagcatcggc aattccggcg ctcgctcctt
     3361 gggccgacgc gattgccatc gcagtggtgg ttatcccgat ctcctaccta tcgctggtgt
     3421 tcggtgagtt ggtacccaaa tcgctcgccc ttcgcttctc tgaacagatc gcctgcatgg
     3481 ctgcccgacc cattgatctt ttgtcgcgcc ttacctcatt gcttgtgaaa tttctgactg
     3541 ggtccagcaa tgcggtgctt cgcttgttcg gcggatccga agtcgaaggc gcgagtttta
     3601 tcagcgttga ggaggtgaaa tccctgatcc gcgaaggagc ggccaaaggt atattcaacg
     3661 aaactgagaa agagctgatc catggcgtct ttgagctggc cgacacaccc gtgaaagcgg
     3721 tcatggtgcc gcgcaccgaa attcacgcgc tggagattca ctccagcccc agcgaagccg
     3781 cggcgagttt catcgaaagc ggcttctcgc gcatccctat ctatgacggc gagctcgatc
     3841 atgtggtcgg ggttctttat aacaaagatt tctttaagac tctgcaacaa aaaactgagt
     3901 ttcggataaa agatcacctg cacccggcgt ttttcgtccc gagcacgctg ccgataagcg
     3961 agctgctgaa gcagctgcaa cggcggcggc ttgcgatggc aatggtggtc aacgaattcg
     4021 gcgaggtcga gggattggcg acgcttgaag atttgctgga ggagatcgtc ggcgaaattc
     4081 gcgatgagta tgatcgtgaa gagcgcggcc cggtggaccg attgcctgac ggctcgatgg
     4141 tgatccaggg ttcggcgctg ttgaaagatt tgaaaaccga cttcgacctg ccgttcgagg
     4201 agtcacccga ttaccatacg cttgcgggtt tcctgctcgc taagcttaag cgcattcccc
     4261 gcggcggtga atgggtcgaa gaaaatggct ataagcttac cattgtcgat atggaaggaa
     4321 ggcgtattgt caaaataaaa ctggagcaga cgaatccgca gaaacagctc tgatccttgt
     4381 taatagcaac ggatctctat ttcattgccg tcgggatcga ggaagtaaac ggatgttgct
     4441 ctgccacggg cgccccagcg cgacaccggt ccttctctga tgatgatact attctctttc
     4501 aagtagtcga cgattccggc gaaatcctcc ttgccgatga ccatgcagaa atgattcaaa
     4561 ttgcccgccg ttttttctcc atcactcggc ctactctgct gttcccggac tggaaacaaa
     4621 tcgatgatgg tatcggtatt gatgcgcacg gacggaaagc cggccttgcc ggtgcggaat
     4681 tcgtcgagtc gttccgcttt caagcccaag actccggtgt agaattgcaa agagcgctca
     4741 atgtcactga cattcagaac gatatggtcg agctcgctga ttttaaatcc gctcatgatt
     4801 tgtctcctcg gtttgcgacg ggtcacccga ctccttctga atcgctgcga tctcctgcaa
     4861 caacctttcg acatcttcag gcgtgccgac cgtgatgcgc aagctgtctc gcagttccgg
     4921 tgtatcaaaa tagcgcacgt aaatctttct ttcccgtagc gcctggtaaa gcgccgtttg
     4981 ctcctgccct ttccgttgcg ccattacaaa attggcgtgg gatgggtaga cgtaaaaacc
     5041 cagcttgacc aatccttcga tcagctgccg acgcgtttgc tggatgcggc ggacgttgcg
     5101 ctgcatccag ggaagatcat gcaacgcagc cgtagcggcg accaagctca gccggtttac
     5161 gttgtaactg tcttttacct tcatcatccc cgcgatcaat tcgatagggg caaaagcaag
     5221 gccgatccgc agccccgcaa gagaaaagga ttttgagaaa ctccgtagca cgatcagatt
     5281 gggaacactt tgaatcaaag gaatagccga tgaatcaggt tcttcagcga aatcgatgta
     5341 agcctcgtcg actacgataa ctccagcgac agcccgagcg agcctttcaa tctcggttag
     5401 acttaccagt gttccagacg gcgagttggg attacataaa aaggtaacgg ctgcctttgc
     5461 cgagtgtaac tccggcggaa ttaaatagtc ggcatgataa tcgacgcaca cctgctggcc
     5521 atcttgaatc ttcaccagag tctcatagag cgagtacgtg ggcaccggga atgccactcg
     5581 atcgccgctc ccgacaaaac aacgcaagac aatcgacaga atctcgtcgg agccgttgcc
     5641 ggctagaata ttctgcggtc cgacaccata aacctccgct gcaatgttgc gcaagctatc
     5701 gctgagcggt tccggataaa gcctgagcga ctggtttgtc gcttcacgta gggccgcgag
     5761 cacgcccgga gagggcgggt acgggttttc attcgtgttg agcttcgtca gcttttcatc
     5821 accaggttgc tccccaggaa cgtagccggc catcgacgcg atgttggggc gaaaataacg
     5881 tgacatggtg cggattctag ctgtcgcaaa aactcatttc aaaccacaac cgacgagcta
     5941 ttccgaggta agcgccattg aacagcttta gcttgggcta aaagttttgt ccaaatttaa
     6001 tcgattttgt ccagcgatag ctcgtttgtg cacgatttca cacgattctg cgtggccggc
     6061 gtttgtaagg tcttggaaac taagcatttt cgtttttaac actggcatgt ttttggcaca
     6121 ctgcccgcag cgttcgtgct gctgtgagaa gtaccgccac tttcaagaga ctgccgagat
     6181 ttgctccgag cgtttgttgt tgagcaacag atcacgtgcc gagattgcgt gagaataggc
     6241 agcctcaatg cttactccgc cccaggggta agtggcgctc ggagtaaggc cgcaaagtga
     6301 attgagcagc gttggaggaa agtatgcaca accgcaatca atgcgagatc gatgaagtgg
     6361 ccgccttgca acaagcaatc ggagctaagg aattgctgtt gcgagagcgc gaagctgcat
     6421 tcgaagctct ggaacaacgg ttaaactcga ccatcgaaca gctgagtgcg gacttgaatg
     6481 aaaaacaaac tttgatcggt caaaacgaaa tgagatctcg gcaagcgaag gcagacgtta
     6541 ttgagattgt ggaacagaag acccgcctcg agatgcttca gaagcaaacc gaacgcttgt
     6601 tatctgctca ggccgagcaa atccgtgccg gagttcgcgc cgagatccag tatctggaga
     6661 ggcagctcat ggacaaggag tcggagctgc aaaaatgcca agcctgcacc atcgacctgc
     6721 aacatcgatt agcggaaatg cagttgctca gtaagagccg cgccgtgcag atcgaagatc
     6781 ttaaagcaga aaccgttcgt ctttcacaag atatcagtcg tcacgagtct gtgaagctcg
     6841 gaaccggttc ttacgccgat catgagctcc gatatggccg ggagcccgag gcctgggcag
     6901 gagagatatc ggccaagctg gacacaaaca gccacgatgc agagatcgca gaagcaccgc
     6961 cacggaacgc gatcagtacg ctgcgccaag aggcggaaga aaaacatctt ctcctcgcca
     7021 gccgcaatga agaactagtg cgcgtgaaag ccgagctgga ccttcttcaa ggccggatta
     7081 gcgagctcaa cgcaagcaat gagcgtattc aggaatcggc ggaaactgaa tgcgcaaaga
     7141 tgcgcgccga gtttcaagct caactggctt ttctacaggc ggagctgagc caaagagatt
     7201 gggcgctcga agaacgcgaa gcagcgcaga aggtgctcga gcaaggactc cgggcgaaga
     7261 tcggccaatt ggaacttcag ctggcacaga taaaaagctc ggctgagggt ccggctcagg
     7321 aattcgttct cggcgagaac tgggaatcag aagcgcggtt agatcatacg tggaagctgc
     7381 aagagcggct cgacggaaat ggcgccgtag gcgcgcagac cccctccgat cgatctggca
     7441 aatggcgcgg cagcggccgc tggaaaagac gctggagata gcgtaaacgg cgcggaactc
     7501 gcctccgccc gatagattca tccgcgcaaa ggacaaccat gataatgacc gaacgagtga
     7561 cgaccggcat cccaggattg gatcaacttc ttgaaggagg ctttcccaag ggccgttcta
     7621 ttctcgtcac cggcgagcct gggactggga agacaatctt cgcattgcaa ttcttggtcg
     7681 aaggcttgag gcgtggagaa aagggcatct acgtcgcggc ggatgaaggt ccgaccgata
     7741 tcatagaaca ggccgcctca cttggatggg acctagagaa atatattgcg gcgaaggagc
     7801 tggcgatact caacgctgga acttatctga gctcctcgcc cggcaccgga aaagagcggc
     7861 agatcgatat tcataaagcg atcggtgacc ttgccggctt cgttaaccgc ttaggagcaa
     7921 aacggctggt gctggatccg gctggaccgt ttgttctgct gcgcgacacc gcaactcgca
     7981 ttcaggatca gacccgcttg ctgatcaagc tattgcggac atcgatgcaa acaaccaaca
     8041 tcctcacatc ctacggcgtg ccgcgaaccg gcgaacgcac tctgcatgga atcgaggaat
     8101 acctggttgc gggcgcgatc atcctggaaa tcatctggag ggacggcaag ttggaccgca
     8161 gcatggtgat tgaaaaaatg cgctgcaccg atgtcaagcc ggcgcagcac gcattcgaca
     8221 tcaacaaagt tcaggggttt gtcctcaatc ccctgcccta agaatctcac aggtgacaat
     8281 cgctatggat cctcagcgtg tttccaccgg tgtatccggt ttagactctc agctcattgg
     8341 cggcttcatc aaaggccgtt cctacgttat cctcggcgac gctggaacgg gtaaaaccac
     8401 cgcctgcctg cagttcctgc taagcggtct caagcaggga gagaagggac tttacatcac
     8461 tgtcgatgaa cgaccgacgg aaatcatcga gtccgcagca tctctggggt gggattttga
     8521 atcctacacg cacgataaaa gcctggtgat gctcgatgct tcgccttact tcagcgggcg
     8581 cgccggcacc tcacctgaca agagcgtgga tctgcagaag attgtttcag atctagccgg
     8641 ctatgccaaa agaattgatg ctacgcggct tgtgattgac ccggtcacgc cgatgatttt
     8701 ttccgactca ccgagtcgag ttcaggatca ggcgcgttct ttcattcacc tcgtgcactc
     8761 gcaattgaat accaccaacc tgtttagcgc ccaatactct agtcgggccc ccgaccccag
     8821 ttttggcatc gaggaatttt tagtttcggg cgtcttgcac ttgaaaacga ctccagtgga
     8881 cggcggattc ggtcgcaagc tcacgattaa aaaaatgcgc ggcactccca tcgagcccag
     8941 cgagtactgg tttgcaatca ccgcgggcga gggcatcgtt ttgactccag aacgggagcg
     9001 gcccgcggcg gtccaggaat ccggccatac gacattgctg gaatacttcg agcctcccgc
     9061 caaagcaaag taatcaacct cttcggctcc gcgctctaga acaaagtctt cgtttctcct
     9121 ccctcttgca cgtggcataa acccgccggt agcggcatcc actagctgga tgccagattg
     9181 gaggcaaccg tcggttttct tgacaccctc ccaaatgatg attaactctc ctacatataa
     9241 ggagaaaagt atgcgtctag acaagctcac cacgaaatcc caggaagctc tgcaacacgc
     9301 gcaagctctg gctgaaaagc gcaatcacca ggcaatcgat gtcgagcacc tgctgtttgc
     9361 tttgctgggg cagaaagagg gcgttgttct ctctttgttg caaaagcttt ctgtgccact
//
LOCUS       DASUTG010000242         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_38776, whole genome shotgun sequence.
ACCESSION   DASUTG010000242 DASUTG010000000
VERSION     DASUTG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38776"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1108)
                     /locus_tag="VF089_05465"
     CDS             complement(74..1108)
                     /locus_tag="VF089_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6173442.1"
                     /translation="MKVGLIGPRGAGKTTIFNMLTGLNAQIGGFAGKEEIHLGVIKVP
                     DPRIDKLSHIFKPRKTTYAEIRFTDFPASQGEDNLKSNNAIVAQMREVDAIALVLGNF
                     EPDAKPLRELNHFLTEMILADLAIIENRRSRLKKEKARPLEENLLQRTAAALEGEESL
                     RNLTFTADEESLLAGFGFLSRKPLLVLLNQSEEQLGQALDPNYLEELKRRGLEGLALA
                     AKAEMEIAQLDEADRITFLKDIGIEEPARDRFIRACYRLLDLISFFTSGEDEVRAWTI
                     TQGTVARKAAGKIHSDIERGFIRAEVVAYEDFIALGSESKCKEAGKLRLEGKDYVVKD
                     GDIVHFRFAV"
     gene            complement(1314..1784)
                     /locus_tag="VF089_05470"
     CDS             complement(1314..1784)
                     /locus_tag="VF089_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173443.1"
                     /translation="MITKKRFEAQVSNNLSNNHSQGRSPFWVKSPGQSNATSEEKAFA
                     VFQSDILIPEQFLATYQRTFRLDPEKVLMLAVLQDAVTCFQENIGATCKRKRALYQEA
                     EEWFIVEDKSYLFSFENICEALGFDVSYLRQGLLRWKEKAAERQSSQPVTRAAG"
     gene            complement(1942..2295)
                     /locus_tag="VF089_05475"
     CDS             complement(1942..2295)
                     /locus_tag="VF089_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173444.1"
                     /translation="MKSSFRIKKSLMFSAILISLTWGAFAMSLAYESASDVGLYGVSS
                     YNASAEGLLRSEPFSPRLAVICLSGDVPRSAQPHFQAYRISYVRLIDPVRFPFFLENH
                     VPKVSLQILQSVLLI"
     gene            2488..3684
                     /gene="ribB"
                     /locus_tag="VF089_05480"
     CDS             2488..3684
                     /gene="ribB"
                     /locus_tag="VF089_05480"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX6173445.1"
                     /translation="MLKSSTMPLATIEEAIDDIRRGKMVILMDDKDRENEGDLCMAAE
                     KVTPQAINFMATHGRGLICLTLTEERVRLLNLSMMVSENTSPFGTAFTVSIDSVLGIT
                     TGISAADRAKTILDAIADDAKPQDLVTPGHIFPLRARNGGVLVRAGQTEGSVDLARLA
                     GLKPAGVICEIMKDDGTMARQPDLMRFARKHKLKVVTIADLIQYRLNYDSLVYQAASA
                     PLPTRMGGQFQAIVYNTHVDQSEHLALIKGQISPKEETLVRVHTKYVPGDVFGFELLN
                     TGRVIQSSMEIIAREGKGVILYLQPEHKGLSPAMVTYPTVQGKQQKNINLSFIYRADF
                     KEYGIGAQILRDLGVRKMRLLTNNKKHLVGLRGYGLEVTSLMPIPRDNPLREAAKQKR
                     TKTRNP"
     gene            complement(3795..>4031)
                     /locus_tag="VF089_05485"
     CDS             complement(3795..>4031)
                     /locus_tag="VF089_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173446.1"
                     /translation="LPLPPGARGLIVDAGYSVRVVITGDPVGERYVDINFTDAPALDY
                     WVDGQLVRERVFSNVGEALKRIRAELPKYLKKSE"
BASE COUNT         1023 a         1078 c         1020 g          910 t
ORIGIN      
        1 cagtttcaga agctgagatg ttcctagtac gaccgtagtc ggtgacgggc agcttcagcg
       61 aaggagctcg accctaaacg gcaaagcgga agtgaacgat atcaccgtcc ttgacgacat
      121 aatctttgcc ttcgagacgc agcttaccag cctccttgca tttggactcg ctgcccaacg
      181 cgatgaagtc ttcataagcc acgacttcag cgcgaataaa gccacgctca atatcgctgt
      241 ggatcttgcc cgcggctttg cgtgctacgg ttccttgagt aatggtccag gctctgactt
      301 catcctctcc agaggtaaaa aaactaatca gatcaagcaa gcgataacag gcacgaatga
      361 agcggtctcg cgcaggttcc tctatgccga tatctttaag aaacgtaatc cgatccgctt
      421 cgtccaattg cgcgatctcc atctcggcct tggccgccaa ggcaagaccc tccaagccgc
      481 gccgtttcaa ctcttccaga taattaggat ccagcgcttg tccaagctgc tcttcggact
      541 ggttcaacag gacaagcaga ggcttacgac tcaaaaaccc gaaaccagca agcaagctct
      601 cttcatcggc agtgaacgtc aagtttcgga ggctttcctc gccttccagg gcggcagcgg
      661 tgcgctgcaa cagattttct tcaagcggcc gcgctttttc tttcttaaga cgcgagcggc
      721 ggttctcgat gatggcgagg tcggccagga tcatctccgt gagaaaatgg ttaagctccc
      781 taagaggctt ggcatcgggc tcaaagttgc ccaagactag ggcgatggca tcgacctcgc
      841 gcatctgtgc gactatcgca ttgttggact ttagattgtc ctccccctgg ctcgccggaa
      901 agtcagtgaa gcgaatctcg gcatacgttg tttttctagg tttgaagatg tgcgaaagct
      961 tatcgattcg gggatcagga acctttatca cccccaaatg aatttcttcc ttgccggcaa
     1021 atccgccgat ctgggcattt aacccagtca gcatattgaa aatagtcgtc ttaccggcgc
     1081 cacgggggcc gatcaatcct actttcatag tgcctgatcc ctcgaatgac cagcgggttt
     1141 ggtgcgcgct cgcaggtcta gcagaagcta gtaagcgagc cgatgatttg ccggcggatg
     1201 ctattcaccg gtttctcttg gccagtttct ccgcgcatgt tcctatgcgg ggatcgggca
     1261 tctcggcttc agaacccaat cagcaatgcc caaaccgccc gacctgctgt ttcctagccg
     1321 gcggcgcgcg ttaccggttg gctactttgt ctttccgccg ccttttcctt ccagcgcagt
     1381 aatccttgcc gcaagtaact gacatcaaag cccagcgcct cacaaatgtt ctcaaaggag
     1441 aaaaggtagg atttgtcctc gacaataaac cattcctcgg cttcctgata aagcgcccgt
     1501 tttctcttgc acgttgctcc aatgttctcc tgaaagcaag taacagcgtc ctgcaagacc
     1561 gcgagcatga gtaccttctc aggatcaagg cggaaggttc tttgataagt ggcaaggaac
     1621 tgctccggaa tgagaatgtc agattggaaa acagcaaacg ccttttcttc agaggtggca
     1681 ttggattgtc cggggctctt gacccaaaaa ggcgaccgac cttggctatg attattcgaa
     1741 agattattcg aaacctgcgc ctcgaatctt tttttcgtaa tcacgggaaa cctccattct
     1801 aattagatct gatacaaaca gtgttcgtgg cgatcacgcc tgcgtctaag gagcagacgg
     1861 ataatgcaag aacttcaacg ccgccctgac aaaaaacccg gatgggctac tgtcgagcga
     1921 aacaaccgtt gggggaaaag ttcaaatcaa cagcactgac tggagaattt gaagggagac
     1981 cttgggcacg tggttctcta aaaaaaaggg gaatcttacc ggatcaataa gccggacgta
     2041 actgatccga taagcttgga agtgcggctg tgccgaccgc gggacatcac cactcagaca
     2101 tataactgcg agtctcggtg aaaacggctc ggatctcagc aatccctcgg cggaggcatt
     2161 gtacgatgaa acgccgtata gccctacgtc cgaagcggac tcgtaggcga ggctcatggc
     2221 gaacgcgccc cacgtaaggg atatcaggat cgccgagaac atcaggcttt ttttgattcg
     2281 aaaggaagac ttcacgctaa ttcaccgacc acacgctaac atccaccgac cgaaagtcaa
     2341 cagtgcctct agtcttttac ggcacaaaga gtttcgcacc gtgactcagg tgcaaattca
     2401 atcgcctgcc cgctaataat ttacggtatc agcaaacgac cgacgattct cgtttgccaa
     2461 gaggactgtt tgaccgaacc aggtaagatg ctaaagtcat ctactatgcc tttggcaact
     2521 attgaagaag cgattgatga tattcgccgg gggaagatgg tcatcctgat ggatgacaaa
     2581 gatcgcgaga atgaaggcga tttgtgcatg gcggcagaaa aagtcactcc ccaggcgata
     2641 aattttatgg ccactcacgg ccggggactt atttgcctca ccctaactga ggagagggta
     2701 agattgttga acctctccat gatggtatcg gaaaatacct ctcccttcgg caccgccttt
     2761 acggtgtcga tcgactccgt gctgggtatc accaccggaa tatcggcagc tgaccgggcc
     2821 aagaccatcc ttgatgccat tgcagatgat gccaagccgc aagatctggt tacgccggga
     2881 catatttttc cattgcgcgc acgcaacggt ggcgttctgg tccgagcagg tcagaccgag
     2941 ggctcggttg atttggcgcg tttagccggc ctgaagcccg ctggagtcat ctgcgaaatc
     3001 atgaaagatg acggtacgat ggcacgccaa ccggatctga tgaggttcgc tcgcaaacac
     3061 aagttgaaag tagtgaccat tgccgacctg attcaatatc gacttaacta cgactcattg
     3121 gtttatcagg cggcgagtgc acccctgcca acgcgcatgg gcggacagtt tcaggcaatc
     3181 gtctataata ctcacgtcga tcaaagcgag caccttgcct tgataaaagg ccagatatct
     3241 cccaaagaag agactttagt gcgagttcac acaaaatacg tacccggaga tgttttcggc
     3301 tttgagctgc tcaataccgg gagggtgatt cagagttcaa tggaaatcat cgccagagaa
     3361 ggaaaagggg tcatccttta tttgcaacct gagcacaagg gcttaagtcc cgcgatggtt
     3421 acatacccaa ccgttcaagg taagcaacaa aagaacatca atctttcgtt tatctatcgc
     3481 gcggacttca aagagtatgg gattggtgcg cagatactgc gcgacttagg cgtgcgtaag
     3541 atgcggctgc ttaccaataa caagaagcac ttggtcggac tgcgcggata cgggctcgag
     3601 gtgacatctc tcatgccaat cccgcgcgat aacccattgc gtgaagccgc caaacaaaaa
     3661 agaaccaaaa ctaggaaccc gtgaagaccg agctcttgaa ggcttcgggg tcaattacat
     3721 ccggaacaaa agttcaatgt cgaactgcgc cggcggctga gtcgaatacg ctcgagccta
     3781 cagcgcagcg cggtctattc cgatttcttg aggtacttgg gtaattcggc gcgaattctc
     3841 ttgagcgcct cgcccacatt tgagaaaaca cgctctcgga ccaattgccc atcgacccag
     3901 taatccaatg ccggcgcatc ggtaaaattg atgtccacgt accgctcgcc aacagggtcg
     3961 ccggtaatga caacccgcac tgaataaccg gcatcaacga tcagtccccg ggcaccggga
     4021 ggtaacggca a
//
LOCUS       DASUTG010000243         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2114339, whole genome shotgun sequence.
ACCESSION   DASUTG010000243 DASUTG010000000
VERSION     DASUTG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114339"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1043
                     /gene="proC"
                     /locus_tag="VF089_05490"
     CDS             231..1043
                     /gene="proC"
                     /locus_tag="VF089_05490"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6173447.1"
                     /translation="MITEKIGIVGAGKIGSAIARGIITAGLVATDQVMASDVSDSLRH
                     AIGNELGIAVTSDNRQLCDFADVVILAVKPQIIDSVVKQMAKSFDAGKLLVSVAAGVP
                     LSRIESGLAPGTRVVRVMPNICCVVGAGAAGYAGGAHATPQDLEIVGSILNSFGIGLA
                     VEEKDLDAVTGLSGSGPAYVFLFIEALADGGVKVGLSREVALKLALHTVYGAARMALA
                     SAKHLSELKDEVTSPGGTTIAGLYALEQKSLKGMVMDAVVSATKRSEELVKG"
     gene            complement(1108..1689)
                     /locus_tag="VF089_05495"
     CDS             complement(1108..1689)
                     /locus_tag="VF089_05495"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEX6173448.1"
                     /translation="MWGRVTVVLLLLLSACVTVNIYFPAAAVQRAADEIVKETWGGPS
                     GKTEQIQPQSLLRFAPRGILSLGLVREAFAQEADINVSNPAIRGLRESIKRRSGAIKP
                     YMDKGNAGINQDGMLVVRSSEGLGLKERAEVQQLIEAENRDREALYTEIAKANNIPPD
                     AVPKIKGIFAKSWIDQAQSGWWVQDSQGNWKRK"
     gene            complement(1701..4697)
                     /locus_tag="VF089_05500"
     CDS             complement(1701..4697)
                     /locus_tag="VF089_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173449.1"
                     /translation="MAPLIVVPGFGEFEQCCETTLVARLIISVYVLAALKRYLITSAL
                     VFVTLITMVYGTLFVIFNSSRFLMWSERELSIRTGYEITLGDIKFGLPFRITGTALII
                     AKSNHVLATAASVAMSLNPLDLLYKKIQRLTIERPVIHLELEEILTPGAQASSPLRIR
                     HLEIRDGSVVLSTAEGKHIEFSAINLRAESLNLGESSDISLNADVPWLNAKAELFFTQ
                     QNEQTKVDLFLREASSGHSLPLTGSEKVAKEVLRLQAKLTRNPNGTANLAATGKFEDL
                     RIGPKPLTGVIDARVVPNADFTEATVSARVEIADIRNIIGELSDVPRGAAVATLMGSY
                     SATGKLLSITASELTSPMGTAHLNGSVSTESDFVVNRARLSVDSVPWQALKTLLPEPL
                     KHWTYHGTAAAELTFDGSWTSLRVSGAVTSSKFIVQGSNFSLQDIALHAPLDWNASSL
                     KFKNVSIKGQSLASGAKDRTQAGAEQVQIEGSLDYTMGAPLVANGRVRINGGRFASAD
                     GTKIGENLLLSGTIEVASSTARHPVQVTGKLSLERGEMLWGKFFADLGGQRPMLDFHG
                     DYLSNDDSVRLRQARLTLLSTGSIEVSGTINQLARRPSVEIRAHSEEFRVGDFFNFFV
                     QENFKRQYPSIDKLTVGGQLAFQLHARGNLDRLVTEGRMTLQAGELRGKSDEWEIGPV
                     ALTLPFQISYPERSQNAARTPPGILSIRGGRFGSRSLEPITSVVSLSNNTLIFHQPVR
                     LVIFGGRLEIANLAWPDVINDPKSMSFALDLRLLQLQELTDALGWPRFSGTLSGAIPQ
                     IRSTGDTLRSEGQVQVDLFGGQLQIEQMEIENLFSAIPSLKLAARFQDIRLEQVSDTF
                     SFGKISGILEGAVNDLVITEGQPARFRAHAETVERPGSSQWISVEALNKITILSSGQD
                     SNVLYGGLASLFDSFRYSKMGFKASLRNDKLILRGIESRDGKEFLVVGTLLPPTVNII
                     SHTQEIGFSELLRRLERITTEKPQVK"
     gene            4969..5706
                     /locus_tag="VF089_05505"
     CDS             4969..5706
                     /locus_tag="VF089_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6173450.1"
                     /translation="MQNNKYLALTALGFFLAGCTGMTRNQAATVGALTCGALGAGAGV
                     YAAHQGINGKHRSEGAGAAIGAVSGALLCGGLAYLIAEDPKPAPKPPPPPPPPPAPKP
                     PPPPPPPPPPPPPPPPPAKKPTPPPPPAPKVERTIILDDVLFDFDRSNVKPEAAKILD
                     RLVAFMQENKNSKVTLSGHTDNVGSDAYNQKLSERRVAAVRDYVVKKGVDAGRISGQG
                     FGESKPIADNKTREGRSKNRRVEIKVN"
     gene            5740..6123
                     /locus_tag="VF089_05510"
     CDS             5740..6123
                     /locus_tag="VF089_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173451.1"
                     /translation="MPGKMIVFFLASALFFPVLAPAQNFTFKEIEARVLEYRKWLDQV
                     GSTGSRYWIRLDSSKRPHKLYVGEGFMEADPDEKENFVEIFSRFLAGHPEKNMLIDIY
                     DATNGKPIGEYGFGGFKLFVSTSHR"
     gene            6216..>6509
                     /locus_tag="VF089_05515"
     CDS             6216..>6509
                     /locus_tag="VF089_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX6173452.1"
                     /translation="MKPVFALRQDAREIFKAGLKAADAAKAVSRHLRVDGEAIEIDGR
                     LYSLTNYRKIFVVGAGKGSAQMCRALAELMGDWLYCGIIITKYGYAVPVKKIAM"
BASE COUNT         1501 a         1722 c         1743 g         1543 t
ORIGIN      
        1 ggaggcggaa agaaagttcc gggaagtctt ggcgttcagc ccggacgatg tggaagcatg
       61 gagaggcctc caatccatcg gtaagaagta ttagatcagc tttgcagtct cattcaaacg
      121 aaggcccacg gcaagttgcc atgggccttc gccttttgtg ttcctggtga ctttgcagtt
      181 cctgccggca cgctctatct tagcgagctg aatggctgaa ggagggttta atgatcactg
      241 aaaaaatcgg catcgtcggg gcagggaaga ttggttcggc gattgcacga ggtatcatta
      301 cggcgggtct tgtagctact gatcaagtca tggccagcga tgtcagcgat tctttgcggc
      361 atgccatcgg caacgagttg ggaatcgctg tgacatcgga taatcggcag ctctgtgact
      421 ttgccgatgt cgtaatcctt gcggtcaagc cgcagatcat cgactccgtc gtgaagcaaa
      481 tggcaaaaag ttttgacgca gggaagctgc tggtttcggt ggccgccggc gtgccgcttt
      541 cgcggattga atccggcctg gcgccgggca cccgtgttgt ccgtgtgatg cccaatattt
      601 gctgcgtggt tggcgcgggt gccgcgggtt acgcgggagg ggcgcacgct acgccgcagg
      661 acttggagat cgttggttcg attctcaaca gttttgggat tggcttggcg gtagaggaaa
      721 aggatttgga cgcggtcacc gggctgagcg gtagcgggcc cgcctacgtt ttccttttca
      781 tcgaggcgct ggccgacggc ggggtcaaag ttggtttatc gcgggaggtg gcgctcaagc
      841 tcgcgcttca tacagtctat ggcgcggcgc gcatggcgct cgctagtgcc aaacacctga
      901 gcgagctcaa agatgaggta acttccccgg gcggcactac catcgcaggc ctatatgcct
      961 tggagcagaa atctctaaaa ggaatggtga tggatgccgt tgttagcgcg actaaacgct
     1021 cggaggaact ggtgaaaggg taaccaaagc tgagtggaag gttgatttct attttcacct
     1081 cgtaactatc agcgcgtcgg accgtgctta cttcctcttc cagttccctt gcgagtcctg
     1141 gacccaccaa cccgactgcg cctgatcgat ccagctcttg gcgaatattc ctttaatttt
     1201 cggcaccgca tcgggaggaa tattattggc tttcgcaatt tccgtataaa gcgcttcgcg
     1261 gtcgcggttc tcggcctcaa tgagctgttg cacttcagcg cgttctttaa gacctaatcc
     1321 ttcgctgctg cgaaccacta gcatgccgtc ttgattgatg ccggcattac ctttgtccat
     1381 atagggtttg atggctccgc tgcgccgctt tattgattcg cgcaatccgc ggattgccgg
     1441 attggacaca ttgatatcgg cttcttgggc gaaagcctca cggacgaggc cgagcgaaag
     1501 gataccgcgc ggggcaaatc gcaggaggct ctgaggctga atttgctccg ttttcccgct
     1561 tggccccccc catgtctctt taactatttc atcggccgcg cgctggaccg cggccgcggg
     1621 aaaatagatg ttgacggtaa cgcaggcact aagaagaagt aacaaaacca ctgtaactcg
     1681 tccccacatc attcctccat ttatttgact tgcggcttct cggtcgtgat ccgctctaat
     1741 ctccgcaaca gctcagagaa gccgatctcc tgtgtgtggc tgataatatt aaccgtgggt
     1801 ggcagtaggg tgccgaccac cagaaattct ttgccgtccc ggctttcgat tcctcgtaaa
     1861 atcaatttgt cgtttctcag gctcgctttg aaacccattt tgctgtaacg gaaactatca
     1921 aacaaactgg ccaagccgcc gtaaagcaca ttcgaatcct gcccggagct cagaatagtg
     1981 attttgttca aggcctcgac gctgatccat tgactgctcc ccggccgctc gacggtttca
     2041 gcgtgcgctc gaaaacgcgc tggttgacct tcagtaatca cgagatcgtt aacagctcct
     2101 tccaaaatgc ccgagatctt gccaaatgaa aaggtatcgg aaacctgctc caaacgaatg
     2161 tcttgaaaac gtgcggccag cttcagcgat gggatcgcgg agaacagatt ctcgatctcc
     2221 atctgctcga tttgcagttg cccgccgaac aggtctacct gtacctgccc ctcgctgcgc
     2281 agtgtgtctc cggtcgagcg aatctgcggg atggctccgc tcaatgttcc gctaaagcgc
     2341 ggccagccca gagcatcggt caactcctgc agttgtaaaa gccttaagtc gagagcaaaa
     2401 gacatcgatt tcgggtcatt gataacgtcg ggccaggcta aattagcgat ctcgaggcgg
     2461 ccaccgaaaa ttaccaggcg aaccggctgg tgaaaaatca acgtattgtt cgataaggac
     2521 accacgctgg tgatcggctc tagtgaacgc gagccgaacc ggccgccacg aatcgagaga
     2581 atcccagggg gagtgcgagc ggcgttctgt gatctttccg gataactgat ctgaaacggc
     2641 aaggtaagag caactggacc aatctcccac tcatcagatt tcccgcgcag ttcgccggct
     2701 tgcaaagtca tgcggccctc ggtaactagg cggtccaaat tgccgcgagc gtgaagctga
     2761 aaagcgagct gtccgccaac ggttaattta tcgatgcttg ggtattggcg tttaaaattt
     2821 tcctgaacaa aaaaattgaa gaagtcgccg acacggaatt cctcgctgtg cgcccggatt
     2881 tctaccgatg gtcgccgcgc cagctgattg atcgtgccgc taacctcgat gctgcccgtg
     2941 gacaaaagag ttaaccgggc ctgacgtagc cgaacgctgt cgtcattgct tagataatcc
     3001 ccgtggaaat ccaacatcgg tcgttgtccg cccagatcgg cgaaaaactt cccccataac
     3061 atttcaccgc gttctaacga aagcttgccg gtgacctgca cgggatggcg cgccgtactt
     3121 gaggcaacct cgatggtgcc cgacaacagg agattctcgc caatcttagt gccatcagct
     3181 gacgcaaagc ggccgccgtt gattcgaact cgaccgtttg cgactagcgg cgctcccatt
     3241 gtgtagtcaa gcgacccctc gatctgcacc tgctcggcgc cggcttgagt gcgatccttt
     3301 gcgcctgagg ccagcgattg tcctttgatc gagacgttct tgaactttag cgatgaagcg
     3361 ttccaatcga gcggcgcatg taaggcgatg tcctggagag aaaaattact gccctgaacg
     3421 ataaatttac tgctagtcac tgcgccgctt actcgcagcg acgtccagga tccatcgaag
     3481 gttaattccg ctgcggccgt tccatgataa gtccaatgct tcaaaggttc cggcaacaag
     3541 gttttaagcg cctgccatgg cacactatcg accgacagtc gcgcacgatt gacaacgaag
     3601 tcgctttcgg tgctgacact accattgaga tgagccgtgc ccatcgggct ggtgagttcc
     3661 gatgcagtta tggacaggag tttaccagtc gctgaatagc tgcccatgag ggttgccaca
     3721 gctgcaccgc gaggaacgtc gcttagttca ccgatgatgt ttcggatatc ggcaatctca
     3781 acacgtgccg atactgtagc ctcagtgaaa tccgcattgg ggaccacgcg agcatcaatg
     3841 acgccggtca gtggtttggg gccgattctc aggtcctcga atttgccggt tgccgccaaa
     3901 ttggctgtcc cgttcggatt cctggtaagc tttgcctgca gacgcaacac ttctttcgcg
     3961 actttctctg aaccggttaa gggaagcgaa tggccagacg acgcttcgcg aagaaacaga
     4021 tcgaccttcg tctgttcgtt ttgctgtgtg aaaaacagct ccgctttggc atttagccat
     4081 ggcacgtcag cattcaagct aatgtcgcta gattccccga ggtttagact ttcggcgcgc
     4141 aaattgatcg cagagaattc gatgtgttta ccctccgctg tacttaaaac gacggagccg
     4201 tcacggatct ccaggtgacg tattcgcagc ggtgaagatg cttgagcgcc gggtgtcagt
     4261 atctcttcta attccagatg aatcaccggg cgttcgatcg tcagtctctg aattttctta
     4321 tacaggagat ccagaggatt gagactcatc gctacgctgg cggcagtagc taggacatga
     4381 ttggattttg cgatgatcag cgcggtaccg gttattctaa atggcaatcc gaacttgatg
     4441 tcgcctaaag tgatttcata accggttcta atcgaaagct cacgttccga ccacatcaga
     4501 aatcgtgagg aattaaaaat tacaaagagc gttccataga ccatggtgat gagagtgaca
     4561 aagacgaggg ctgacgttat aagataacgt ttcaaagcag ccagaacata cacgctgatg
     4621 atgagacggg caactaacgt ggtttcgcag cactgctcga actctccaaa ccctggcact
     4681 acgatcagcg gcgccaatct gtggcagcaa ccattttgag cgaactgtat cgggattgag
     4741 ttttcccgac gccgaattca ctccgaaaac ttttttcact actattgttt tgactactgg
     4801 ttgtagtgag aggacgtccc ctccctctag atctagttca ccttcaaata catacgcgta
     4861 gtaaccttaa agattacatg gagtttctaa atgcttaaat tttcttgaca agcacagatt
     4921 cctatactac gttagccgaa atttttacta cttttcgggg aggcagagat gcaaaacaat
     4981 aagtatttag cgttgaccgc gctaggcttt tttttagctg gttgcacggg gatgacgcgt
     5041 aatcaggccg cgacggtcgg cgcattaaca tgcggcgcct taggcgccgg tgctggcgtt
     5101 tacgctgcac atcaaggtat caacggtaaa caccgcagtg agggagccgg agccgcaatc
     5161 ggcgcggtta gcggcgcact gctttgtggc ggcctggctt atctgattgc tgaggaccca
     5221 aaaccagcgc caaaaccgcc accgccaccg cctccaccgc cggcgccaaa gccaccaccg
     5281 ccgccaccgc cacctccacc accgccgccg ccaccaccgc caccggcaaa aaagccgacg
     5341 ccgcctccac caccggcgcc gaaggttgag cgaacgatta tccttgatga cgtactcttt
     5401 gacttcgata gaagcaacgt taagcccgaa gcggctaaaa ttcttgatcg tctggtagct
     5461 ttcatgcagg agaacaagaa ttcgaaggtc acattgtcgg gccataccga caacgtgggt
     5521 tcggatgcct acaatcagaa gctttcagag cgccgggttg ccgccgtgcg ggactatgtg
     5581 gtcaaaaagg gtgttgatgc tggacgcatc tccggacagg gttttggcga gagcaaaccg
     5641 atcgccgata ataaaacccg agagggccgg tcaaagaacc gccgggtcga aatcaaagtc
     5701 aactagaaat caactgcgtt ggagggattc tcaatgcgga tgccaggaaa gatgattgtt
     5761 tttttcctgg catctgcatt gttttttccg gtccttgctc cggcacagaa ctttacgttc
     5821 aaggaaatcg aagctcgagt tctggaatat cggaagtggt tagaccaagt cggatccact
     5881 ggttcgcgct attggatccg cttagacagc agcaaaagac cccacaagct ctatgtgggc
     5941 gaaggtttca tggaggcaga tcccgacgaa aaggaaaatt ttgtcgagat cttctcgcgc
     6001 tttctcgccg gccatcctga aaagaacatg cttatcgaca tttacgatgc caccaatggc
     6061 aaacccatcg gtgagtatgg ttttggcggt tttaagcttt tcgtctctac ctctcaccgc
     6121 taacgcaacc cgcagtttac cgctgtatct ttcttgccaa gagcgacggc ataccaaagc
     6181 tttcattcgc ttctgcattt ttgtactaca gcggcatgaa gcccgtattc gcattacggc
     6241 aagatgcccg tgaaatattc aaggcggggc taaaggccgc agatgcggct aaagctgttt
     6301 cacgtcacct cagagtcgat ggcgaggcta tcgagatcga tggccggctt tattctttaa
     6361 cgaattaccg aaagatcttc gtcgtcggcg cgggtaaagg ctccgcccag atgtgtcgcg
     6421 cactcgcaga attgatggga gattggcttt attgcgggat catcataact aagtatggct
     6481 atgcggttcc agtgaagaaa atcgcgatg
//
LOCUS       DASUTG010000244         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1965383, whole genome shotgun sequence.
ACCESSION   DASUTG010000244 DASUTG010000000
VERSION     DASUTG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965383"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VF089_05520"
     CDS             complement(<1..1465)
                     /locus_tag="VF089_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6173453.1"
                     /translation="MKLGTKLIVSLVVIILVTMTVHGYLSIQQDRENAMREMRVGMRG
                     FSRAIHAVLRDLYADSHNLNATQEFANTVGPRGNIHGLIVYNADAERVAVSSSLQYPD
                     DFAELHPAAILELDPRPALGEPNAIEGYVRESGILVYYRIEPFTASDGRIAGAFVLAR
                     QGSQLISNIEARRQRIIGTSAALVILLSVLILIIVRRNITLPINQLVQRVREIGRGRW
                     GQRIEIKGRDEIAALAEEFNIMSEELNRSYSKLLEEQQQKLKLERELRHSERLASVGH
                     LAAGLAHEIGTPLSIVRGRAEYLLRRPRSSEELTGNLEVIRSQSDRITAIVRQLLEFS
                     RRREPMFRSVDLSELINHVRYLLEHQLFDKSLNVEIHVSPKPLLIQADPDLLQQVFIN
                     LFANSLHALGPKGAIKIRASSAPSIDGQGGVRIVFEDNGIGIAPEIIDHVFDPFFSTK
                     DVGEGAGLGLAVTYGIINEHGGEITVESEPGKFTRFLI"
     gene            1863..1985
                     /locus_tag="VF089_05525"
     CDS             1863..1985
                     /locus_tag="VF089_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173454.1"
                     /translation="MKKLLFALLLGVTVVSSIVDLATPVSAQDEDPKPKKPEGD"
     gene            complement(2071..2346)
                     /gene="yccX"
                     /locus_tag="VF089_05530"
     CDS             complement(2071..2346)
                     /gene="yccX"
                     /locus_tag="VF089_05530"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX6173455.1"
                     /translation="MPQARIRLIVKGRVQGVYFRASTVQQARHLGLTGWVMNRDNGSV
                     EVVAEGRSESIEELIAWCRHGPPGARVDEVDLQRHGFRGEYNEFRIR"
     gene            2578..>2721
                     /locus_tag="VF089_05535"
     CDS             2578..>2721
                     /locus_tag="VF089_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6173456.1"
                     /translation="MGAKLYVGNLPYSVTEERLQQHFAQHGSVISARIVTDKFTGRSK
                     GFGF"
BASE COUNT          651 a          724 c          716 g          630 t
ORIGIN      
        1 gaatgaggaa gcgagtaaac tttccaggtt cgctttctac ggtaatctcg ccgccgtgct
       61 cgttaatgat gccgtaggtt accgccagcc cgagtccggc tccttcgccg acatctttgg
      121 tagaaaaaaa aggatcgaat acatgatcaa tgatttccgg cgcgatacca atcccgttat
      181 cttcaaagac aattcgaacg ccgccttggc cgtcaataga gggcgcggat gaggcgcgga
      241 ttttgatcgc tcccttgggg ccaagcgcgt ggagagaatt ggcgaacaaa ttgatgaaca
      301 cttgttggag cagatccgga tcggcttgaa tgagcaatgg cttgggcgag acatggatct
      361 caacgttaag gctcttgtcg aagagttggt gttcgagaag ataacgcaca tggttgatca
      421 actcagataa gtcgaccgat cgaaacatcg gttcccggcg gcgggaaaac tccagcagtt
      481 ggcgcacgat ggcggtgatg cgatcgcttt gcgagcggat cacctcgagg ttaccggtaa
      541 gctcttcgct gctgcgcggc cggcgcaaca gatactcagc tctgccacga acgatgctca
      601 agggagtgcc gatctcatgg gcaagacccg cggccaagtg acccaccgat gccaaacgtt
      661 cagagtggcg cagctccctc tccagtttta gcttttgttg ctgctcttcc aacaatttgg
      721 aataactccg attgagttct tccgacataa tattgaactc ttcagccagc gcggcaattt
      781 catcgcgacc tttgatctcg atgcgctgcc cccaacgacc gcggccgatc tcgcgaacgc
      841 gctgcaccag ctgattgatg ggcagggtga tattgcggcg tacgatgatc agaatgagga
      901 cagacaagag aatgaccagt gccgcgctgg ttccgatgat tcgctgacgt cgcgcttcga
      961 tgttgctaat caactgagat ccttgacgcg ctaagacaaa ggcgcccgca atccgaccat
     1021 cgctcgcggt aaacggctcg atacggtagt agaccaaaat tccactttcg cgaacataac
     1081 cctctatggc gttgggttcg ccgagagctg gtcggggatc cagttccaga atggcagccg
     1141 gatgcagttc ggcaaaatcg tcaggatact gaagcgagga tgagaccgct acgcgctcgg
     1201 catcagcgtt atagacaatc aggccgtgaa tatttcctcg tggccctacc gtattggcga
     1261 attcctgagt tgcattaaga ttgtgactat cggcatacag atcccggaga acagcgtgga
     1321 tggctcggga gaaaccgcgc atgccgaccc gcatttctct catggcgttt tctcgatcct
     1381 gctgaatact gaggtagccg tgaactgtca tcgtcaccag gatgatgacg accaatgaga
     1441 cgatcaattt tgttccaagt ttcatgcacg cagtttcgca gtcatcgaag ggagcaaggc
     1501 ctgtaccgcg tcgtcgctaa tcgcaaacgg cgaaaagatt aaagtctcgg catacttcgc
     1561 acaaaaaata gcgacgaact ctgtggcaac ttacgacagc gtgtggtttt tcacaatcat
     1621 tgaaatttcg ttcgtttgtt caagcaattg agactattcc aaaagcgcac aggttcttag
     1681 cgcgttgcat attacaacgg aaagtattgc cgaccctagc tcaaagccac tgaacattcg
     1741 cgttattcca agaacatcag ccgaggttcg cgggcacgtc gcttgcgtct attcccggat
     1801 acgaatcggc tattcgggag tatacaaact ttcagattcg aatttagaaa ggaggagctg
     1861 atatgaaaaa attgcttttt gcactgctgt tgggagtgac cgtcgtgagt tcgattgtcg
     1921 atctcgccac gccggtgagc gcgcaagacg aagatccaaa acctaagaag cccgaagggg
     1981 actgatccgc agaagttgat ctcagcttac aacaaggggg ctttgtgccc ccttgttgtt
     2041 tatgacaggc ctcgtgagat ctgctgtcgg ttatcttatt cgaaactcat tatactctcc
     2101 tcgaaacccg tgccgctgca aatcgacttc atccacccga gctcccggcg gtccgtgtcg
     2161 gcaccaggcg atgagttctt cgatgctttc ggatcgtcct tcggccacga cctccactga
     2221 accattatcg cgattcatga cccagccggt taaacccaag tgccgcgcct gttgaaccgt
     2281 agaagcgcga aaatagacac cctggactcg acctttcaca atcaacctga tacgcgcctg
     2341 gggaatggtt ggcatcgctg ctggttttct aacgcagcac ctgctttggc gcaagccgtt
     2401 gattcagtaa tcactgcggc gaaattattt gacaaccgcc ccgcagtttc gataggctct
     2461 cgccggttct aagggtttac cgaagagccc gcgccgtggt cctccagacc gatttaggcc
     2521 ccgccctttg gcgactcgat ccgcccaacg cgtactctga atcaaaagga gggtctgatg
     2581 ggagccaaat tgtatgtcgg caacctgccg tactcggtaa cggaagaaag attgcagcaa
     2641 catttcgcac agcacggctc ggtgatcagc gctcgtatcg ttaccgataa attcacgggg
     2701 cgttccaaag gtttcggctt c
//
LOCUS       DASUTG010000245         8411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1373459, whole genome shotgun sequence.
ACCESSION   DASUTG010000245 DASUTG010000000
VERSION     DASUTG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373459"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..935)
                     /locus_tag="VF089_05540"
     CDS             complement(408..935)
                     /locus_tag="VF089_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6173457.1"
                     /translation="MGQLDLGALFVHFLILWFIAIGGPSTILPDIHRYLVEVHHLLSD
                     AEFVEIYTLAQVAPGPNAMYVTLIGWHLAGWKGVAATTIPLLIPATTLTLLVGHLNER
                     YPNARGARAIRRGLMPITIGLVFASATILMRAVNHDWHGYLITLLTVAVVLRQAWNPL
                     WLFAAGALAGIAGFV"
     gene            complement(935..1489)
                     /locus_tag="VF089_05545"
     CDS             complement(935..1489)
                     /locus_tag="VF089_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6173458.1"
                     /translation="MTQTESHKPRVSQLELFLAFSRISLSSFGGAIFWTRRELVERQR
                     WLTDREFVDVYTLGQLLPGPNVLNLTVIVCHRFAGWTGAAAGVAGFLGWPCLMVVGMG
                     VLYQNYGGLPYVQQALTGMSSVAAGLLLATVIKLATVLPRHWRPWLFGILAFAAVGIM
                     RWPLLWVMGALAPWAVFAAWKEPE"
     gene            complement(2226..2801)
                     /locus_tag="VF089_05550"
     CDS             complement(2226..2801)
                     /locus_tag="VF089_05550"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173459.1"
                     /translation="MPLLITALLPRASPRKRKNLCTSSSSASPWNNQLPFRIAIAKGF
                     LKDQGLTVEQIFVRGGPTAIAALVSGNVEFASIGGAQAPVRSNVRGLDLQIIASSSNH
                     TNYALLGSKEAKNVEELRGKIVGVTGAGAFSEIAIRIFLRETTSIPTMTSPCGRSAEP
                     HCGPSRSKEASSPPRRLPPRIPSGCSTRVYR"
     gene            2989..3951
                     /locus_tag="VF089_05555"
     CDS             2989..3951
                     /locus_tag="VF089_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX6173460.1"
                     /translation="MFLGRVLVEEFQADVLEWLRQRAEVVAVNPWIEPERWNHEAPRA
                     NAVISRKGKITRVHMEESRGRLKIIARTGVGVDPSRVDLAAARQYKIWVTNMPGSNAV
                     SVAELVFGQMIALVRHTHEANRAVKENRWGDYLNYLGTELAGKTLGIIGMGNIGARVA
                     LRARAFEMSFIVYDPYIPESHVTALGGRWLSLDELLGESDFLTVHCPLNDETERMIAA
                     RELALMKPSAYLINAARGGIIDEQALLAVLRECKIAGAALDVIESEPPRKNHPLFSLG
                     NVLWTPHLGAVTLEASARGEWGAAQEVIRVLEGKRPKNPVNELS"
     gene            complement(3965..4693)
                     /locus_tag="VF089_05560"
     CDS             complement(3965..4693)
                     /locus_tag="VF089_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6173461.1"
                     /translation="MSRDQHPEAIIVHSSDLHLGTDDSFSERDSLAVLPKVLSAANEA
                     RADIVVLAGDTFDNHRQPRDLLERAAQILRDYGDPIVILPGNHDPLTPDSVYRRGGLA
                     QIPNVHILGLNVDQSVMFPEFELEIWGRAHLDYTDMSPLADPRPRSTRWQLAAAHGHY
                     VDEQRDPNRLIGSWLIHREELLATDADYVALGHWNQATPVGSGEVTAYYSGSPEYAGT
                     VNIIRLTSRGAVEVSQAPLNGTLR"
     gene            complement(4960..5238)
                     /locus_tag="VF089_05565"
     CDS             complement(4960..5238)
                     /locus_tag="VF089_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173462.1"
                     /translation="MKRFLSVLVASMFLASAAFAQTGKSAEKKSSGSMQSESVKTDGK
                     EKSESVKSQGKAKGKSKTETTSQTDTKSTDGKATSETKSSSKSKAATK"
     gene            5466..6923
                     /locus_tag="VF089_05570"
     CDS             5466..6923
                     /locus_tag="VF089_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX6173463.1"
                     /translation="MAERLRIAVGSGEHSLLPQMANRHGLIAGATGTGKTVTLRVIAE
                     GFSRIGVPVFMADVKGDLSGLARAGQENPKIRERLEKLGVKEFDFAPYPTVFWDVYGQ
                     HGHPVRTTISDMGPLLLARLLNLNETQSGVLNLVFKVADDNGMLLLDLKDLRSMLQFV
                     GDNAAKFRTKYGNVSTASIGAIQRGLLALEEQGGENFFGEPALELDDIIQTDSAGRGV
                     INILTAEKLIHSPKLYATFLLWLLAELFEQLPEVGDPEKPKLVFFFDEAHLLFNDAPA
                     VLLEKIEQVIRLIRSKGVGVYFVTQNPLDVPDIVLGQLGHRVQHALRAFTPRDQKAVK
                     AAAQTFRQNSAIDVEAAITELGVGEALVSFLDEKGRPTIVDRALIYPPQSQLPPLAAG
                     ERESIVKQSTIYGHYEKRVDRESAHEMLKARAPQPEQAPAKTAGTSWQSEAGGLFDAM
                     GKSAARAIGSQVGREIIRGVLGSLFGGATTRKRRY"
     gene            complement(7200..7850)
                     /locus_tag="VF089_05575"
     CDS             complement(7200..7850)
                     /locus_tag="VF089_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEX6173464.1"
                     /translation="MRSWIKPALFIACLIPLAQLVYGFYTDDLTANPIEYITRFTGSW
                     ALIILITSLAITPLRKIFGWNTLIKYRRMLGLFAFFYAVLHFSTYMVLDHFFDFQAIA
                     KDIFKRPYVTAGFTSFVLMIPLALTSTTAMIRRLGKRWQQLHRLVYIAAAAGVLHFYW
                     LVKSDIRRPAQYGAVLALLLGVRLAYALLPAVSKRFGAKTKTITPQTGRSRSTSAR"
     gene            complement(8027..>8411)
                     /gene="msrP"
                     /locus_tag="VF089_05580"
     CDS             complement(8027..>8411)
                     /gene="msrP"
                     /locus_tag="VF089_05580"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017942067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX6173465.1"
                     /translation="PYVEGLRMDEAMHPLTILSVGLYGQVLPAQNGAPIRVVVPWKYG
                     FKSIKSIVKIRFVEKQPPASWNMMQPQEYGFYSNVNPEVDHPRWTQATERRIGEFLRR
                     KTLMFNGYGDQVAQLYSGMDLKKFY"
BASE COUNT         1926 a         2200 c         2443 g         1842 t
ORIGIN      
        1 atgggttttc gatcatcttt atccagaatc ctgataggtt tcgatgccgg taccactgta
       61 ataaaagcag ctcgcatcat cgatgatgct tgcgcgccgc atttaccccg ctctgcttag
      121 gaaaatagca ccctaagcct cgaagttata ccactctgta aaaaatcaaa gacattggag
      181 actaaaattt cacggtgacg aattcttcgg ggtgagtttt gcttttcaac gcaggcgctt
      241 gccgcgggct ttgccacgct gttttgcttg ctcttgattt tagcagctgc cttttgacgg
      301 catcgacgtc gcgcctaacc gattcagact agcgctactc accacctcag ggctgtgttg
      361 tgttcactgc gccgtagtca gcagtcggtt gccgttgcgc acgatgctca gacgaacccg
      421 gctatgcccg ccagtgcgcc cgcggcgaac agccaaagag ggttccatgc ttggcgcagg
      481 acaacggcga ccgtgagcag cgtgatcaag tatccgtgcc agtcatggtt taccgcgcgc
      541 atcaagatcg tcgcgctcgc gaaaacaagt ccaatggtaa tcggcatcaa accgcgccga
      601 atcgcgcgag caccccgggc attgggatag cgctcgttga gatgaccaac gagcaaagtc
      661 agagtggtag caggaatcag cagcgggatc gtcgtggcgg ctacgccctt ccatcccgcc
      721 aagtgccagc cgatcagagt cacgtacatg gcgttagggc cgggtgccac ctgcgccaag
      781 gtatagatct cgacaaactc cgcgtcgctc agcaggtggt gcacttccac caaatagcgg
      841 tgaatgtccg gcaggatcgt cgagggaccg ccgatcgcga tgaaccagag gattagaaaa
      901 tggacaaaca acgcgccgag gtcaagctga cccatcactc cggctctttc caagccgcga
      961 acacagccca gggcgcgaga gcacccataa cccacagcag cggccaacgc atgatgccga
     1021 ctgcggcgaa tgcgagtatg ccgaacaacc agggccgcca atgccgcggc aacaccgttg
     1081 ccaacttgat cacggtggcg agcagcaacc ctgcggcaac gcttgacatt ccggtgagcg
     1141 cctgttgcac gtaaggcaaa cctccgtaat tctggtagag cacgcccatg ccgacgacca
     1201 tgaggcaagg ccaacctaag aagcccgcca cgcctgccgc ggcaccggtc cagccggcga
     1261 aacgatggca aactattacc gtgagattga gcacattagg gccaggcaga agttgtccta
     1321 gagtgtagac atcgacaaac tctcggtcag taagccagcg ctgtcgctcg accagctcac
     1381 gtcttgtcca gaaaatcgcc ccgccgaagc tcgacaggct gattcgggaa aaagccagga
     1441 acagttcgag ctggctgacg cgcggcttgt gtgactctgt ttgcgtcatg gtgtttcgct
     1501 tgcaattgaa atcaactggg atctgctcag cacgagaaca tcgggattag aaagtggcgg
     1561 agtgtatcga gccaacagct aatcggaaat cgaattggcc gagcaggttc tgccttgggt
     1621 ataattacga gcgatagcta ggaaagcgga gctaactcga tctagactcg aggctgcttc
     1681 ttctcgcata tcgtgccaat ttctcaggtg agaagttacc attcaatctt actcatttgc
     1741 cagcatcgta agggaaaaat ttctaattag cacgaatttt aaggaaactt tcgaaagacg
     1801 aacagtcgag ggtatataaa agcgggtctt ttaactcact agctgaggcg ttttttagcg
     1861 gtgattggcg gcgacagaac taaacaacgt tcgggtaggc atgtgcaact ccgagctacg
     1921 gttcgtctac ctcacggttt caaacgcctt tcgtggcgca gctcctgttg cgcttgtcta
     1981 aggatgcggt ccgttgatga cgcccggact gaagagatgg taggaacggc tggcgacatc
     2041 cggatgtcct gtaaatccgg cggcaaagcc ggtctgggcg cttctttttg gtgctgtttg
     2101 gcaattgcat tcccaggatt acggctttcg gaaagcgctt ggtggtctcc ggattttttt
     2161 ccggcatctc accccgggtg acgaagatgc gctgcgggat cgcagcgtct cgttcatatt
     2221 cacgatcatc ggtaaaccct tgtcgagcaa cctgaaggta tcctcggcgg taaacggcgc
     2281 ggcggcgatg atgcctcttt cgagcgcgat ggcccgcaat gcggttccgc cgatcgcccg
     2341 cagggtgacg tcattgtcgg gattgaggtt gtttctctga gaaaaatcct gatggcaatt
     2401 tcggagaacg cgccggctcc ggtgacaccg acgatcttgc cgcgcaattc ctcgacgttt
     2461 ttggcttcct tgctgccgag aagcgcgtag ttcgtgtgat tggatgacga ggcgatgatt
     2521 tgtagatcga gaccgcggac attgctccgg accggcgctt gggcgccgcc gatgctggca
     2581 aactctacgt tccccgaaac cagagccgcg atcgccgtag ggccgccgcg cacgaagatt
     2641 tgctcgacgg tgaggccttg atctttgaga aagccttttg cgatggcgat gcgaaagggc
     2701 agttggttgt tccatggaga ggctgacgaa gacgatgtgc agagattttt tctcttgcgc
     2761 ggcgatgccc gcggcaataa tgcggtgatc aataagggca agacctagag agatggtcta
     2821 agcagcatgc atcgtttcat gcttgcctac cgaggatcca atagttgatt tccgcgtatt
     2881 ccgaaaagat cagtcgcgcc ttatactatc gcgactcgtg gcgaacgagc cattagaggc
     2941 aaagctgtta acaccaccac taccatgtag cggaaacagg aggaataaat gtttctcgga
     3001 cgagtgttgg tcgaagaatt tcaagccgat gtgctcgagt ggttgcggca gcgcgctgaa
     3061 gtggtggccg tcaatccctg gattgagccg gagcgctgga accatgaggc gccacgagcg
     3121 aatgcggtaa taagccggaa gggaaaaatc acgcgcgttc acatggagga gagccgcggc
     3181 cggctaaaga tcatcgcgcg caccggcgtc ggtgtcgatc cgtcgcgggt cgatttggct
     3241 gcggctaggc aatacaagat ttgggtcacc aatatgcccg gcagcaacgc cgtgtccgtg
     3301 gcggagttgg tgtttggcca aatgattgcc ctagtgcgcc acacgcatga ggccaatcgc
     3361 gccgtgaaag aaaatcgctg gggcgattac ctcaattacc tcggcaccga actggcgggg
     3421 aaaacgttgg gaatcatcgg catgggaaac attggcgcac gggtggcgct gcgcgcccgg
     3481 gccttcgaaa tgtccttcat cgtctatgat ccctatattc ccgaatctca tgtcacggcg
     3541 ctcggcggcc ggtggctaag cttggatgag ctgcttggtg aatctgattt cctaacggtt
     3601 cattgtccgc tgaatgacga aaccgagagg atgattgcgg cgagggagct ggcattgatg
     3661 aaaccttctg cctatctgat caatgcggcg cgcggaggta tcatcgatga gcaagcttta
     3721 cttgcagtat tgcgagagtg caaaattgcc ggcgcggcgt tggatgtcat cgaaagcgag
     3781 ccgccgcgta aaaatcatcc gctgttcagc ctcggcaatg tcctgtggac gccgcacctg
     3841 ggcgccgtga cattggaagc gtcggcgcgc ggcgaatggg gcgctgcgca agaggttatt
     3901 cgggttttag aggggaaacg tccgaagaat ccggtaaatg aactgtccta agaatcccgt
     3961 gattctagcg caaagttccg ttcagcggcg cctggctaac ttcgaccgca cccctggatg
     4021 tgagccggat gatattgaca gtgccggcgt actcaggcga gccggagtag taggcggtga
     4081 cctcgccgga gccgacggga gttgcctggt tccaatggcc caacgccacg taatcggcgt
     4141 cagtggcgag caattcttca cggtgaatca gccatgagcc gatcagccga ttcggatctc
     4201 tctgctcatc gacataatgc ccatgcgccg cggcaagctg ccagcgagtc gaacgcggcc
     4261 gagggtcggc aagcggcgac atatcggtgt agtcgaggtg tgctcgaccc cagatttcca
     4321 gttcgaattc ggggaacatc acggattgat caacattcag accgaggatg tgaacgttgg
     4381 gaatctgcgc aagcccgccg cgccggtaaa ccgaatccgg tgtcagcggg tcgtgattgc
     4441 cgggcaaaat aacgatgggg tcgccataat cccgcaaaat ctgcgctgcg cgttcaagaa
     4501 gatcgcgagg ttggcggtga ttgtcaaaag tatctccagc gagtacgacg atgtcggcgc
     4561 gcgcttcgtt ggccgcgctg agaactttgg gaagaaccgc taaagaatcg cgctcgctga
     4621 agctgtcgtc ggtgccgaga tgcaaatcag aactgtggac gatgatcgct tccggatgct
     4681 ggtctcggga catagataag atcccacttt caatgtgcaa caataccgat cccttattct
     4741 tatgggcaca gcgggagaat tgcaagcaac agcagcgctg ttagttgggc ttaattaaaa
     4801 cctgacgatc gcttggtctc ggcctggtga tgggctcagc gcttgccagt gctcggatgg
     4861 aggagataga tcgctgatcg gaaaatttaa ggctgggaca tgggcgcaac agtgtagcca
     4921 ggtgcggagg acacctggct accatcttga aatcaaagct tatttggttg cggctttgga
     4981 tttgcttgag gacttggtct cacttgtagc tttgccatcc gtcgatttag tgtcagtctg
     5041 gcttgtggtt tcggtcttcg atttgccttt agcttttcct tgacttttta cggattcaga
     5101 cttttcttta ccatccgtct ttaccgactc ggattgcata ctaccggaac tcttcttttc
     5161 tgcactcttt ccggtctggg caaaagctgc cgaagctaaa aacattgaag caactaacac
     5221 gctaaggaaa cgtttcatgt gtgatcctcc agtatgatat ctgttgttgt ttaagaacgc
     5281 ccgagatcga ccgcgccgcc ctccggtcgg ctcgaactcg tctcgtccct tcttgctgcc
     5341 ttagacgtgt aacgctgtgc tttaattcac gtcacggctg gtctatacat caagtgacct
     5401 attttgacca ttagttcgtt tagatgggta aattcgattt tccctcaaca aagggggtgt
     5461 atctcatggc tgagaggctg cgcattgctg tggggagcgg cgagcactcg ttactgccgc
     5521 agatggctaa tcgccacggt ttgatcgccg gtgcgaccgg cacgggaaag acggtaacac
     5581 tccgagtcat tgccgaaggt tttagccgca taggggtgcc ggtcttcatg gcagacgtga
     5641 agggcgattt gtccggactc gccagggccg gtcaagagaa tccaaaaatt cgcgagcgac
     5701 tggaaaaact tggcgttaaa gaatttgact tcgcccccta tccaacggtc ttttgggatg
     5761 tgtacgggca acacggccat ccggtgcgca caacaatctc ggatatgggg ccgttgctgc
     5821 tagcccggtt actcaatttg aacgaaaccc aaagcggcgt actcaacctc gtttttaaag
     5881 tcgccgatga caacggcatg ctgctgctcg acctcaaaga cctgcggtcg atgctgcaat
     5941 ttgtcggcga caacgcggca aagtttagaa ccaagtacgg caacgtttct acggcaagta
     6001 tcggcgccat acagcgcggt ttgctggcgc tggaggaaca gggtggcgaa aatttctttg
     6061 gcgaaccggc gctggaactc gacgatatta ttcaaacgga cagtgccggg cggggggtga
     6121 taaacatttt gacggcggag aagctgatcc attcgccgaa actctatgcg acgtttctgt
     6181 tatggttgct ggcggaattg ttcgagcagc tgcccgaagt gggagacccg gaaaagccca
     6241 agctggtatt ttttttcgac gaagcacatt tgttgttcaa cgacgcgccg gccgtgttgc
     6301 tggaaaagat cgagcaggtg attcggctga ttcgctccaa aggcgtcggc gtttatttcg
     6361 tcactcaaaa tccgttggac gttcccgaca tcgtgctcgg tcaattgggt catcgcgtgc
     6421 agcatgcgct gcgcgcgttt acgccgcgcg atcaaaaggc ggttaaagcc gcagcccaga
     6481 cattccgtca gaactccgcc atcgatgttg aagcggcgat cactgagtta ggcgttggtg
     6541 aagcgctggt ttcgttcctg gacgagaagg ggcgacccac tatcgtcgat cgcgccttga
     6601 tttatccgcc gcaaagccaa ctgccgcccc tggcagcggg cgaacgcgag agtatcgtga
     6661 aacaatcgac gatctacggc cactatgaaa aaagggtcga tcgtgagtcg gcgcatgaaa
     6721 tgctcaaagc tcgagcgccg caacccgaac aggcgccggc caagacggca ggcacttcgt
     6781 ggcaatcgga agcgggcggt ttgttcgatg ccatgggtaa aagcgcggcc cgcgccatcg
     6841 gcagccaagt cggccgggag atcatccgtg gcgtgctcgg ctccctgttc ggtggagcaa
     6901 cgacccggaa aagacgttac tagaagtggc cgagacatgc gccttctaac agctattcca
     6961 accagcggct ttccacgcat ctgtcatcga ctgttagatg ctctaacggt tcgaacgacg
     7021 caacgccttt aggaaaatgt tcaagtagtc aaacgcaaga gacggcgcag tcgggttgtt
     7081 tactgcaaaa ggggcgccgc gggatcttcg gcattaatcc aagtaccctg cgctcgcgaa
     7141 tggctggcaa tcaacaacta gaaggagaag ccgctcggcg cagtgccacg gacgaatgct
     7201 taacgggcgc ttgtcgaacg agagcggccg gtctgcggcg tgatggtttt agttttagcg
     7261 ccaaagcgtt ttgacacggc cggcaataac gcatatgcaa gccggacgcc gaggagcaat
     7321 gctaacaccg cgccgtattg cgccgggcgg cgaatatcgg actttaccag ccagtaaaaa
     7381 tggagaacac cggcggctgc ggcaatatag accaggcgat gtaattgttg ccaacgcttg
     7441 cccagacgcc ggatcatcgc cgtcgtcgaa gtgagcgcca gcggaatcat caagacaaac
     7501 gacgtgaatc cggcggtgac atagggccgc ttgaaaatgt ccttggcgat cgcctgaaag
     7561 tcgaagaaat ggtccaggac catataggtt gagaaatgca ggaccgcata gaagaaggca
     7621 aagaggccca gcattcggcg gtatttgatc agcgtattcc agccgaatat tttgcgcagc
     7681 ggcgtgatcg ccaggctggt aatcaaaatg atgagcgccc atgagccggt gaaacgggtg
     7741 atatattcga tcggattagc ggtcaagtca tcggtataaa atccgtacac gagctgcgct
     7801 agcgggatca ggcaggcgat aaagagcgca ggttttatcc aggaacgcat caggttaact
     7861 acagagagac aggacggcgc agggatgagc agactagcat tggatttggc ttctgagcgg
     7921 aattcaaacc tcggaagcag agaggagagt gcaatcattc ccaagcggaa tcttgtgatc
     7981 ttcgtctccc tttgccggtt gctgaagtgc gacttcggcc ttcctcctag tagaattttt
     8041 tcaggtccat gccgctgtat agctgcgcga cctgatcacc ataaccgttg aacatgagcg
     8101 tcttacgccg gagaaactcc cctatgcgtc gctccgtcgc ctgggtccag cgcggatgat
     8161 cgacctccgg attgacgttg gagtaaaagc catactcctg tggctgcatc atgttccaag
     8221 atgccggtgg ttgtttttcg acaaaacgga ttttgacgat ggacttgatg cttttgaatc
     8281 cgtacttcca cggcaccact acgcggatcg gagcgccgtt ttgcgccggc aacacctggc
     8341 catacaaacc caccgataag atcgtcaacg ggtgcatggc ttcatccatg cgcaagcctt
     8401 cgacataagg c
//
LOCUS       DASUTG010000246         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_631917, whole genome shotgun sequence.
ACCESSION   DASUTG010000246 DASUTG010000000
VERSION     DASUTG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631917"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF089_05585"
     CDS             complement(<1..536)
                     /locus_tag="VF089_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173466.1"
                     /translation="MNWRVIWFALSLLVVLGSVKLPQADGRIVVGVSTVNVAFLPIYI
                     TQDRGFFKDEGLDVLVVMFNAGATNLQAVIGGDVQIMAGGVPETVLARAGGLDIKNFW
                     AISNVMPFQIYGSPKMKSLKDSKGKKFAISRFGSLSEFLTRSALRQAGVDPKDVTMLQ
                     IGATPARFTTLATGIVDAT"
     gene            complement(690..908)
                     /locus_tag="VF089_05590"
     CDS             complement(690..908)
                     /locus_tag="VF089_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173467.1"
                     /translation="MNAVKIIGVVLIVAGVLGLAYGSFSFTKETHEAKIGPLELQVKD
                     KETVKIPAWAGVGAIVIGAVLLLVPLKR"
     gene            1236..2513
                     /locus_tag="VF089_05595"
     CDS             1236..2513
                     /locus_tag="VF089_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173468.1"
                     /translation="MRDLFCIFAILICLLPQLAGAQPTLLRIVISKTDTPQLWDGAIA
                     DFEQQNPGVKVVKEIAPNSSTQFHDLLTQKLKNRDTRLDVFFMDVIWPSEFASAGWTL
                     PLDRFFSRAEQGKFLEAPIAANRYRGEIFGVPLFIDAGLLYYRKDLLEKHRMAPPRHW
                     PELVDQAKTILAAERDPHLVGYSGQFKQYEGLVCNMMEFILGNGGALWDEQRLVSTLQ
                     QERAKQAVRFVRDHIIGEISSRGVLAYEEPESVALFAQGRAIFHRNWPYAWRVANDSE
                     QSRVVGKVGMMPLPAFPGGKGASTLGGWQMGISRFSRIPDVAWRFVAFMTSYEMQKRI
                     AFSTGRAPTRKALYDDPELLAKIPWLESLLQTFKQAVPRPTTPVYVPLSNIMQRYFSA
                     ALALPGSKIDQRAALAARDMDRVLDLLREAAAP"
     gene            2510..>2735
                     /locus_tag="VF089_05600"
     CDS             2510..>2735
                     /locus_tag="VF089_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6173469.1"
                     /translation="MRSSHRLALLFIVPSLVFVSVFTLYPILESFRLSFYRMILTLPW
                     LGQKMVGWENYVDLWTDPVAWQALRTTLVFV"
BASE COUNT          587 a          789 c          723 g          636 t
ORIGIN      
        1 gtcgcatcga cgatgccggt ggccaatgtg gtgaagcgcg ccggggtggc gccgatctgc
       61 aacatcgtga catctttggg atcgacgccc gcctggcgca gcgccgaacg ggtgagaaat
      121 tcagagagcg agccgaaacg gctgatcgcg aactttttcc ctttgctgtc cttcagcgat
      181 ttcatcttgg gactaccata gatttgaaac ggcatcacat tggagatcgc ccagaaattt
      241 ttgatgtcca gtccgccggc tcgggcgagc acggtctccg gcacgcctcc cgccatgatc
      301 tgcacgtctc cgccaatgac tgcctggaga ttggtagcgc cggcattaaa catcaccacc
      361 agaacatcca ggccttcgtc cttgaagaag cctctgtcct gagtgatgta gatcggcagg
      421 aacgcaacgt tcaccgtcga aactccgaca acgatacggc cgtcagcttg tggaagcttg
      481 acgctgccca gcacaaccag aagcgataaa gcaaaccata ttactcgcca attcattagc
      541 tttccctccg cctgcgcacc gtaggttagt catgaagacc cgtcaagagc catcggcgag
      601 gtcccagggg aaacttcggt cagcaatgtc ttaatggatg cgcgtagtcg tctggctaaa
      661 gcggccttaa tgctatgatg cgatttccgc tacctcttca acggcaccag gagcaagact
      721 gcgccaatca ctattgctcc cacgccggcc caagctggaa ttttgaccgt ctccttatcc
      781 ttcacctgga gctcgagcgg accgatcttc gcttcatgag tttcttttgt gaagctgaaa
      841 cttccatagg ccagccccag aacgccggcc acgatcaaaa ccactccaat tattttgact
      901 gcgttcatct catcccctcc taaaacaatc tcctgtacgt gttagcgatg cgtgcgattc
      961 tctctattcg aatctacgca gatttgtcaa atcaccgatt accagtctcg atgggtcggc
     1021 tggcacacgg ctgctaactt ctcaaaacgc cactatctct ttcccatacc tacggaagcg
     1081 gttcccaagt tccctgccgc taaaaaccga gagattattt gacggccggc aaatgctgga
     1141 catgaccgac atcagattat aggctgccca cgtagtcgaa tgcataaata gagcgccgat
     1201 taacgtaccg ggatgattta ttgagccgga ttttcatgcg agatctcttt tgcatcttcg
     1261 caattctcat ctgtcttcta ccgcaattag ccggcgcgca gccaacactg ctccggattg
     1321 tcatctcgaa aacggatacg ccacagcttt gggacggggc gattgccgat ttcgagcagc
     1381 agaaccccgg ggttaaagta gtcaaggaga tcgcgccaaa ctcttcgacg caattccatg
     1441 atctcctcac ccagaagttg aaaaatcgcg atacccgcct ggacgtcttc ttcatggatg
     1501 tcatttggcc cagcgagttt gcctcggctg gttggacgtt accgctggat cggttctttt
     1561 cgagagcgga gcagggcaaa ttccttgagg cgccgatagc ggccaatcgc tatcgcggag
     1621 agatcttcgg cgtgccgctt tttatcgatg ccggattgct ctattaccgg aaagatcttc
     1681 tcgagaagca tcggatggcg ccgccgcgtc actggcccga acttgtagac caagctaaaa
     1741 cgatccttgc ggccgagcga gatccgcacc tggtcgggta ttcgggccag ttcaaacagt
     1801 acgaagggct tgtctgcaac atgatggaat ttatcctcgg caatggcggc gctttatggg
     1861 atgaacagcg gttggtgagc acgctgcaac aagagcgcgc caagcaggcg gtccggttcg
     1921 tgcgcgatca tatcatcggc gagatctcgt cgcgtggagt gctggcctat gaagagcccg
     1981 agtcggtggc gctgtttgcc caggggcggg caatttttca tcgcaattgg ccctatgcgt
     2041 ggcgcgtcgc caacgattct gaacaatcga gagtggtggg gaaagtcggc atgatgccgt
     2101 tgcctgcttt tcctggtggc aagggcgctt cgaccctcgg cggctggcaa atgggtatca
     2161 gccggttttc gagaataccc gacgttgctt ggcgctttgt cgcattcatg accagttacg
     2221 aaatgcaaaa gcgcatcgcc ttttccaccg gccgcgcgcc gacccgaaaa gcgctctacg
     2281 atgaccccga gctgctggcc aagattccct ggctcgaatc tctgctgcaa accttcaagc
     2341 aagcggtacc gcggccgacg acgcctgtgt acgtacctct gtcaaacatt atgcaacgtt
     2401 actttagcgc cgccttggcc ctgcccggca gcaagatcga tcaacgcgcg gcattggcgg
     2461 cgcgcgacat ggatcgtgtg ctggacctgt tacgcgaagc tgcggcgcca tgagaagctc
     2521 gcaccggctc gccttactgt tcatcgtccc gagtctggtc ttcgtctctg tttttactct
     2581 ctatccgatt ctggaatcct tccgcctgag tttttatcgc atgattttga ccctgccctg
     2641 gctcggtcaa aaaatggtcg gctgggagaa ctacgtcgat ctctggaccg atccggtcgc
     2701 atggcaagca ttgcgaacga cactggtatt cgtcg
//
LOCUS       DASUTG010000247         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_335142, whole genome shotgun sequence.
ACCESSION   DASUTG010000247 DASUTG010000000
VERSION     DASUTG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335142"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="phoU"
                     /locus_tag="VF089_05605"
     CDS             <1..576
                     /gene="phoU"
                     /locus_tag="VF089_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6173470.1"
                     /translation="SLVDRDSSLAEVIIERDHEVNRLDVNIDELCIRLLALHQPAGRD
                     LRFITTGLKITTDLERIGDMAVNICERALELNQEPQLKPYIDIPRMAQIAQRMIQQSL
                     DAFVREDTDLALRVCKDDSEVDQLNAQIFREILTFMIENAQTISRATKISSVSKYLER
                     IADHATNIAEMVIFMVKGKSIRHMKELPPSV"
     gene            579..1301
                     /locus_tag="VF089_05610"
     CDS             579..1301
                     /locus_tag="VF089_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017352921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6173471.1"
                     /translation="MSSIGQKILVVEDEPDIRKLIQYNLTQERYLVVEAEDGEQALKL
                     VQRDTPNLIILDLMLPGLSGLELCKILRERPDTARVPILMLTAKAGEADKVVGLEMGA
                     DDYLSKPFSPREMVARVRAILRRTDSSAAAAETAPSYHKGTLKIDYRTYEVFARGQQI
                     RLTLKEFELLRFLVQNSNRVLSRDQLLDRVWGGETFVTPRTVDVHIRRLRKAVEEDDR
                     RPRLILTLRGVGYKFDEKALAE"
     gene            1279..>2689
                     /locus_tag="VF089_05615"
     CDS             1279..>2689
                     /locus_tag="VF089_05615"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6173472.1"
                     /translation="MRRLWRNSLISKVFLSYLAVVTLLFTSFYLYSSTVVRDFYLSVL
                     GDRLELEARLLAKAVPSLSENREFDVLCRNLASELGVRITMIDGDGRVSCDSVEPSVT
                     MENHRSRPEVLQAYESGSGSSFRYSMTTGEELFYRAFLQNEGGSQRIVRLAIPLNEVR
                     EITDSLRRNLFLGFLVASGVGLLVAFAFSRRFSSRVKRLVEFSQQVAEGVFPQNFFVK
                     SGQDEIHRLELHLNEMSEKIRENVRQVVAEKEKANSILRCMMEGVLVIDPKGRVLVIN
                     EQAQKMFNLAHDTDIRGASILELSRHPEMKKIIEQVLVFDFTSERYSRQVELHEGRWF
                     RVNAVSLRNTQSGSFGSILVFHDITEVKQLERMRSDFVANVSHELRTPLTAIRGYAET
                     LLQNPPNDPADAQYFLGIIEKHSERLSRLTEDLLTLSDLESGKVQLDFKPLDSARVIQ
                     SVLEMFWDQANKKGIKLESV"
BASE COUNT          677 a          637 c          708 g          667 t
ORIGIN      
        1 tcactggtcg acagggattc tagcttggcc gaagtgatta tcgagcgcga tcatgaggtg
       61 aaccgcctag atgtcaacat tgacgaactt tgcattcgtt tgctcgcgct tcatcagccc
      121 gccggccggg atcttcgttt catcactact ggtttaaaaa tcacgaccga tttagagcgc
      181 atcggcgaca tggcggttaa catctgtgag agagcgctcg aactcaacca ggaaccgcag
      241 ttaaagccgt atatcgacat accgcgcatg gcgcagatcg cccagcgaat gatccagcaa
      301 agtctggacg ctttcgttcg cgaggatacg gatctcgccc ttcgggtttg taaagacgac
      361 agcgaagtcg atcagttgaa tgcgcagata tttcgagaaa ttctcacatt tatgatcgag
      421 aatgcgcaaa ccatcagcag ggcgacaaaa attagttccg tgtcaaaata tctcgaacgt
      481 atcgccgatc acgccaccaa tattgccgaa atggttatat tcatggtgaa gggcaagagc
      541 attcggcaca tgaaagaatt gccgccgtcg gtttgattat gagcagcata ggtcaaaaaa
      601 tcctggtcgt tgaagacgaa ccggatattc ggaaactcat ccaatacaat ctgacgcaag
      661 agcgctacct ggtggtggaa gcggaggatg gcgagcaggc tctgaagcta gtacagcgcg
      721 atacaccgaa ccttattatt ctggatctta tgctgccggg gctttccggc ttggagctat
      781 gcaaaattct ccgtgagaga ccggatacgg cacgggttcc cattcttatg ctgactgcta
      841 aagccggaga ggccgataag gttgtcggct tggaaatggg cgctgacgac tatctatcaa
      901 aaccgtttag cccgcgtgag atggtggccc gcgtccgagc cattctccgc cgcacggact
      961 cttcggctgc agccgcggaa actgcaccat cctaccataa agggacgctg aagattgatt
     1021 acaggactta tgaggttttt gctcgagggc aacaaatcag attgactctc aaggaatttg
     1081 agcttctgcg ttttttagtc caaaattcaa atcgcgtctt gagccgcgac caattgcttg
     1141 acagagtttg gggtggtgaa accttcgtta ctcccagaac tgtcgatgtt cacattcgcc
     1201 gtttgcgtaa agcggtcgaa gaagatgatc gcagacccag gttgattttg acgttacgag
     1261 gagttggcta taaattcgat gagaaggctt tggcggaata gccttatctc aaaagttttc
     1321 ttaagttacc tcgccgtcgt taccctactt tttaccagct tttacctcta ttccagcact
     1381 gttgttagag atttctatct atcggtgctc ggtgacaggc tcgagcttga agctcgccta
     1441 ctagccaagg cagtgccttc tctttcggaa aaccgagagt ttgacgtcct gtgtcgaaac
     1501 ttagcaagcg agttgggagt ccgcatcaca atgatcgacg gcgatggccg agtctcatgc
     1561 gattcggtcg agccgtcggt aactatggaa aatcaccggt cccggccgga ggttctccag
     1621 gcctatgaaa gtggctccgg ctcctcgttt cgttacagca tgactactgg cgaagagcta
     1681 ttttaccgcg catttctaca gaatgagggc gggagccaaa ggatagtgcg tttggccatc
     1741 ccgctcaatg aagttaggga aatcacagat tctttgcgtc gcaacctttt cctcggattc
     1801 cttgtcgcct ccggcgttgg cttgctagta gccttcgcct tctcccggcg ctttagctcg
     1861 cgcgtgaagc gcctagtgga attttctcag caggtagcag aaggagtttt tccgcagaac
     1921 ttcttcgtta agtcaggtca ggacgaaatt caccggttgg aactccacct taatgaaatg
     1981 agcgaaaaaa ttcgcgagaa cgttcgacaa gttgtggcgg aaaaggagaa ggcaaattcg
     2041 attttgcggt gcatgatgga aggcgtttta gtgatcgatc caaaaggacg ggtcttggtc
     2101 atcaacgaac aggcgcaaaa aatgtttaac ctggctcacg acacagacat tcgtggcgcc
     2161 tctatattgg aattgtcgcg ccacccagag atgaaaaaaa taattgagca agtcttggtt
     2221 tttgatttca cgagcgagcg gtattccaga caggttgagt tgcatgaagg tagatggttt
     2281 cgggttaatg cggtaagcct aaggaacact cagagtggct cgtttggttc aatactagtg
     2341 ttccacgata tcacggaggt aaagcaactg gaaaggatgc gctccgattt cgttgcgaac
     2401 gtatcccatg aactgcgcac accccttact gcgattcgcg gttatgcgga aacactgctg
     2461 caaaacccgc ccaatgatcc ggctgacgct caatattttc tcggtatcat cgagaaacac
     2521 tcggaaaggt tgagtcgatt gaccgaagat ttgctgacgt tgtccgatct cgaatcaggg
     2581 aaagttcagt tggatttcaa acctcttgac agtgcgcgcg tgatacaaag tgttctcgag
     2641 atgttctggg accaggccaa caagaaaggg atcaagctag agagcgtcg
//
LOCUS       DASUTG010000248         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_188149, whole genome shotgun sequence.
ACCESSION   DASUTG010000248 DASUTG010000000
VERSION     DASUTG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188149"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..771
                     /locus_tag="VF089_05620"
     CDS             376..771
                     /locus_tag="VF089_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173473.1"
                     /translation="MKLPTILRVARATDHLHEVVRFYTEGVGLAQLGAFQDHDGFDGV
                     MLGVPGFPYHLEFTRKRGHVAGRAPTMENLLVFYLPDKHQWQEAVGRMIAAGYQPVPS
                     LNPYWDRDGRTFEDPDGYRIVFQNAAWSA"
     gene            1208..1837
                     /locus_tag="VF089_05625"
     CDS             1208..1837
                     /locus_tag="VF089_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173474.1"
                     /translation="MANTITIPVDAYLVTLQIESYQVVEKKIAVGKSTQIHHERVKET
                     RDIPSNQSDKTVMRAIDVANTIWAPADITFQLRKSFSPITEAPGNAEEVSVSGFFQMI
                     TTLKIKPIGAVAALFVNRFEKRDLGGFAAEALGVCIVTSLGDPVLGKTLAHEFGHLLS
                     LPDLRNDPKSVANNYNLMYEASRAGDKLTSDQISKARNSERVKLVYQSA"
     gene            2294..3307
                     /locus_tag="VF089_05630"
     CDS             2294..3307
                     /locus_tag="VF089_05630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173475.1"
                     /translation="MRFGVEFGSYPAELDTAEVCAQLSLRAQVAYKNNFEALFVAQHY
                     LTGPDAAILQSIPLLAYLAAQVPGMYLGTSIFLLPLHHPVMVAEYISTLDNLSGGKVL
                     FGVGQGYREKEFDSFGIDIRQRRGRLVEALDVIRKLWTGESVTFHGEHFHLDKVISRP
                     TPVQRPGPPLLMGADTVRTVARVPELADHWIASRRHSKSFLREALPQYKAALQRQGKP
                     YRGLFIFRDLCIADTTREAEERIEEGYERRYRRYQQWGQPGERYDLKFDELKRDRLIL
                     GSPSEVVEQVMSYHEEFGAEVMWFMVDWPGMDPRFTLETIERFGKEVIPQIKRLTPEC
                     PLP"
BASE COUNT          810 a          936 c          965 g          830 t
ORIGIN      
        1 ttcacccgga cttcgcaacc cactccacgg ccgttattag accggttcgt tacgaacatc
       61 acgcaacttt cgccagcggc atcgtcgacg gtaagaggcc caggttgaag tgacgggatc
      121 tgactttcgt ccgtctcatc aggtgttgtt aaacggcagg aagattccca gccgattcgt
      181 taacgcgcag ggtattggaa attcaaatat ccgcgactaa ccccggcact cacaaaatta
      241 cgataatcga tcccggggtg gtcgcgtcag ggtcgaatgc gcctaattgg ttatcggcta
      301 cagatagctg gcgcatgaaa aaacaaaact gtgcgtgatt ttttacggtc gaagtgccgc
      361 atcatgaaag acttgatgaa gctaccgacc atcctacgag tggcgcgagc gacagaccat
      421 cttcacgagg tcgtgcggtt ctacacggag ggagtcggct tagcacaact cggagcgttt
      481 caagatcatg acggcttcga cggggtaatg ctcggcgttc ctggtttccc gtaccatttg
      541 gagttcacgc gaaagcgagg tcacgtcgcc ggtcgtgctc ccacaatgga aaatctgctc
      601 gtattctatc tgcctgacaa gcatcagtgg caagaggccg ttggtcgcat gatcgcagct
      661 ggataccagc ctgttccgtc gctcaatccg tattgggatc gtgacggccg gacgtttgaa
      721 gaccccgacg gctaccgcat cgtctttcaa aacgcggcgt ggtccgcgtg agttctcgac
      781 gtagggcaaa atgccgaggt gagagtaaga gcgacgtggc aaccgatgat tttaacaggt
      841 tggcggactt cgcgagctgg cttgttttca gatcggatac aatccagaaa ttcgctcccg
      901 cacttgccca gcgaatcacg tggtagccct gttgcatctt tgtcggaccg ctgattccca
      961 tgctcttgtc cgacggtcga cacttaggaa tgaggcggtt ccaatcattc cagccgttcc
     1021 atcgctccgc taggttctag tttgttatag ctggttaagg cgttcaatct tcaaggttca
     1081 attactgcgt acccttcaga tgtttttcgg caaacgccgg tccagccgtc cttcatcgtt
     1141 tcaaaaatcc cccttgtccg agaccgggca tatccattaa tatccgctta ttcattggag
     1201 gccttagttg gcgaatacca tcaccatacc agtcgatgcc tacttggtca cgcttcagat
     1261 cgagagctac caggtcgtgg aaaaaaagat cgcggttggc aaaagcactc agattcatca
     1321 tgagcgtgtc aaagaaaccc gcgacattcc ttctaatcag agcgacaaaa ccgttatgcg
     1381 cgctatcgat gtagccaata caatctgggc tccggcggat atcacctttc agctgcgcaa
     1441 gagcttttcc ccgataaccg aggcgcccgg aaatgcagag gaagtcagtg taagcggatt
     1501 ttttcagatg attacgactt tgaaaatcaa gccgatcggc gccgtggccg cgttgtttgt
     1561 aaaccgtttc gaaaaaagag atctcggagg atttgccgct gaggcgctgg gtgtatgcat
     1621 tgtcacctcg cttggcgacc cagtcctcgg taagacgctg gcacatgagt tcggccacct
     1681 ccttagcctt ccggacctgc gtaatgatcc caagtcggtg gcaaataatt acaacttaat
     1741 gtacgaagca tcacgcgcag gcgataaact gacctcggac caaatcagca aagcacgcaa
     1801 ttctgaacgg gtaaagctcg tctatcagag cgcttgatcc aaaatcttct tgatgaccca
     1861 aacggcaact agttccaatc gttccagtcg cgaactattg gaaactattt gcatccacgg
     1921 agaagatagc ggtgcagttc cttgacgtca ttgatccact gcagctctag gtacacgcat
     1981 ctgccaactc gaccgaattt ggagcggagt tggagctata cctgccgtgg cggatgttag
     2041 tccgtgcctc ggcaatcgct ctacatggat gagccaagat aacttcagga gttggacatg
     2101 atgcaccggg cctggcataa ccatctgata gacgtatccg ccgacgaaaa agttcgcccc
     2161 tgaggcgctg atttgttgtc tcgtcggtgg ccgaattcat cgataatcct tctccggcaa
     2221 ttcggtgtcc gctgacagac accgtgcagg aaattaaaca aactccattc gttgatttgc
     2281 gggaggagta gaaatgcgat ttggtgtcga gttcggcagt tatccggcgg aattggatac
     2341 cgccgaggta tgtgcgcagc tttcgctacg ggcgcaggtt gcctacaaga acaacttcga
     2401 ggccctgttc gttgcgcaac attatctcac cggcccggat gcggcgatct tacagtcaat
     2461 tcctctactg gcttatctag ccgcgcaggt gccgggaatg tatctcggca cgtcgatatt
     2521 tcttctgccg ctgcatcacc cggtcatggt cgccgaatat atttctacct tggataatct
     2581 atccggtggc aaggttctct tcggcgtggg ccagggctat cgagaaaaag agttcgattc
     2641 attcggcatc gacatacggc agcgccgggg ccgattggtc gaagcattgg acgtgattcg
     2701 caagctctgg accggagagt cggtgacctt tcatggagaa catttccatc tcgataaagt
     2761 gattagccgc cccacaccgg tgcagcgccc gggtccgccg ctcttgatgg gcgccgatac
     2821 ggtgagaacc gtcgccagag tgccggaact agccgaccac tggatcgcca gccggcgcca
     2881 ctcgaagagt tttttacgcg aagcgttgcc gcagtataag gcggcgctcc aacggcaggg
     2941 aaagccgtat cgcgggctgt ttatttttcg cgatctttgt atcgccgata ccacgcgcga
     3001 agcggaggag cgtatcgagg aaggctacga gcggcgctac cggcgctatc agcaatgggg
     3061 acagccgggc gagcgctatg atctgaagtt cgatgagctg aagcgcgacc gcctgatact
     3121 cgggagcccg tccgaagtgg tcgagcaggt gatgtcttat catgaagagt tcggcgctga
     3181 agtgatgtgg ttcatggtgg attggcctgg gatggatccg cgctttacgc tggaaacgat
     3241 agagcgcttc ggcaaagagg tcatcccgca gatcaaacgg ctgaccccgg agtgtccgct
     3301 gccgtaggga tatggcttaa ttcagcacga acaacgatta ttattctcct gctccccgtc
     3361 gttttcttag gatagctttt tcacttcgcg ggattctatc cacgtggtcc gcgtttggca
     3421 actacgttca ccgtggttgc tgcgccgggc gggaacggcg ccaacctctc tggatgtaag
     3481 cgatagaaga acggattaga actaccgtga gagcttagca gtgactcgtt aaacccgaaa
     3541 a
//
LOCUS       DASUTG010000249         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_40372, whole genome shotgun sequence.
ACCESSION   DASUTG010000249 DASUTG010000000
VERSION     DASUTG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40372"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF089_05635"
     CDS             <1..345
                     /locus_tag="VF089_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6173476.1"
                     /translation="LHLCCTANYTRKDTSMKQLSLTEARAKLSRLVAEVDKGKGPFAI
                     AQRSKIKAVLVDAEWHTRVEEELAHYRRTAKRSPLKLKGSMALIGDINQALTEIREER
                     EASFDRLVSGLK"
     gene            342..782
                     /locus_tag="VF089_05640"
     CDS             342..782
                     /locus_tag="VF089_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173477.1"
                     /translation="MIYVTDMHPLVFWSSDRKNPLGKRARKIFQEVEQGKHSIVVPLV
                     VLEETARLIERKVVRLSVPFRRWAEELDRSANFQVQPYTIEILPESVSLGAICDPADK
                     AIVASARQLRCRSSQRMSSFGTAIGSIRCGIELPNNKAALQLKF"
     gene            complement(991..2358)
                     /locus_tag="VF089_05645"
     CDS             complement(991..2358)
                     /locus_tag="VF089_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6173478.1"
                     /translation="MANSVEASGEPQQSSLKRVIGRRMLLLFVLGDILGSGIYVLVGK
                     VGGHVGGMLWLPFLLAFILAVLTAAAYAELAGKYPQAAGAALYVNKAFGIPFVTFIVA
                     FGVMMSGVTSAGVAAQAFGGRYLAEFVSLPVIAGALTFLALVTLVNFVGISESVKVNV
                     TLTVIEASGLLVIVAIGIYALLTGEGEPGRALEFNPREGAFLGLLQGTALAFYALLGF
                     EDSVNLAEEARDPARDFPYALFGGVMVAVLIYMAVAFTATMLVDTETLTKSSGPLLEV
                     VKVAGLAFPPKLFALIALFAVANTALMNMIMASRLVYGMARQRVVPSLFGTVHSTRGT
                     PWVAILFTTAIAAVLVATGDISGLAETTVLLLLCVFALVNISVLVLRRDRVDRKHFRA
                     PTWMPVLGAVVCVILALPITGRDTAVYMRVGILIAIGVVVWSINRLYVGKVKELDPTA
                     IISGN"
     gene            2626..>3443
                     /locus_tag="VF089_05650"
     CDS             2626..>3443
                     /locus_tag="VF089_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173479.1"
                     /translation="MRRSRRGYHWGFLVAALTSAFAANAWGLDRVRIGMGAFSPTNSA
                     IWVAEERGFFKKHGIEAEAIFIGGGAARGVNALLAGDIQFATAGGGAVITSALKGAEV
                     VMVAAQNNKGVHRILVRPEIDTPEEIKGKKIAITTFGSSSHAVLTMVLDVWKIRPDEL
                     QILQVGSSPTMLISLQKKLVDGAVLTDPSYFIAEDHGFRVIADLAKMNIHYLQSMIVS
                     TRSYLRANRDQATRVLKAYVEGIAYFKKNKKESVRIILKKMRMDADQAKYAERT"
BASE COUNT          874 a          953 c          954 g          662 t
ORIGIN      
        1 ctgcatctat gctgtacagc caactataca cgtaaggaca catctatgaa gcagttatct
       61 ttaaccgagg cacgtgccaa gctcagcagg ttggtcgcgg aagtcgataa aggaaaaggc
      121 ccgtttgcga tcgcccaacg atctaaaatc aaagctgtac tggtcgatgc ggagtggcac
      181 accagggtgg aagaggaatt ggcgcattat cgaagaaccg cgaagcggtc ccctctcaag
      241 ctgaaaggga gtatggcgtt gatcggcgac atcaatcagg cgctcacgga aatcagggaa
      301 gaacgggaag catccttcga tcggctcgta tccgggctta agtgatttac gttaccgata
      361 tgcacccatt ggtgttttgg tcttctgacc ggaagaaccc cctcggcaag cgggcgcgaa
      421 agatttttca ggaagtcgag caaggaaagc actcgattgt cgttcccctg gtcgtgctcg
      481 aggaaaccgc gcggttgatt gagaggaaag tcgttcgctt atcggttccg tttcgccgat
      541 gggcggaaga gctggatcgt tctgcgaact ttcaggtgca gccgtacaca attgaaattc
      601 tgcctgaatc ggtttcgctg ggtgccatct gcgatcccgc cgacaaagca attgttgcca
      661 gcgcccggca actccgctgc cgctcatcac agcggatgag ctcattcggg acggcaattg
      721 gatcgatacg gtgtgggatt gaacttccaa acaataaagc ggcgttacaa ttgaaatttt
      781 gaccacagag atccgagact gaatgctcga ttctactccg attctaaagg atatgcccct
      841 gtgtctgcgg actttctctg ggggttgctc agagacctca gcagaatggt actggtaaga
      901 atcggaaaat tactccaaga gtaggcgaga agtacaatac gagggatgag tcgatgtaca
      961 caaaacatgt gatcggctac tgggtgatcg tcaattgccg gatattatcg ccgtcggatc
     1021 gagctctttc accttgccga catacagccg gttgatcgac cagaccacga cgccgatcgc
     1081 aatcaggatg ccgactcgca tgtagaccgc cgtgtcacgg ccggtgattg gcagtgccaa
     1141 gatgacgcaa actaccgcgc ccagaaccgg catccatgtc ggcgcgcgaa aatgcttacg
     1201 atcgacccgg tcgcgccgca gcaccagcac ggatatgttg accagcgcaa acacgcacaa
     1261 cagcaagagc acagtggttt cggcgaggcc gctgatatcg ccggttgcca cgagcacggc
     1321 agcgatggcg gtggtaaaca agatcgccac ccacggagtg ccgcgggtcg agtgcacggt
     1381 cccgaacaaa gatggcacca cccgctggcg agccatgccg tagaccaaac gcgacgccat
     1441 gatcatgttc atgagagcag tgttggccac ggcaaatagc gcgatcagcg caaatagctt
     1501 gggcggaaag gccagaccgg cgacctttac cacttcaagc agcggtccgg aggacttcgt
     1561 cagggtctcg gtgtccacta gcatcgtggc ggtaaacgca accgccatgt aaatcaacac
     1621 cgccaccatc acgccgccaa atagcgcata aggaaaatct ctcgccgggt ccctggcttc
     1681 ttcggcaaga ttcaccgaat cctcaaaacc gagcagggca tagaacgcca gcgcggtccc
     1741 ctgcagcaaa ccaaggaatg cgccttcgcg tgggttgaac tccaacgccc ggccgggctc
     1801 accttcaccg gtcagcaatg cataaattcc gatggcgaca atcacgagca aaccggacgc
     1861 ctcaatgacg gtgagcgtga cgttcacttt gacggactcg gaaatgccga caaagttcac
     1921 caacgtgaca agtgcgagaa aagtcaatgc gccggcaata accgggaggc tcacgaactc
     1981 ggcaagatat ctgccgccaa aggcttgggc ggcgacgccg gcggaagtca caccgctcat
     2041 catcacgccg aacgcgacga tgaacgtcac gaacggaatc ccgaatgctt tgttgacata
     2101 gagcgccgcg ccggcagctt gagggtattt ccccgcgagc tcagcatacg cagctgcggt
     2161 caatacagcc agtatgaagg cgagtaaaaa cggcagccag agcatgcctc caacatgccc
     2221 gccgactttg ccgaccagta cgtagatgcc cgaacccaag atgtcgccta gaacgaagag
     2281 cagcaacatc ctcctgccga tgacgcgctt aagcgaggat tgctgcggct ctcctgatgc
     2341 ttctacgctg ttagccatgt tagtcctatc aagctctcat cattctggta agcagtcagg
     2401 ctgcaactgt acctacagcc aatcttgcgc gactaaatcg tcgcgacgat cgccgtatct
     2461 acaacaacgc tagcctctta cccgccgcgg ttcaagaaag gtgccgcgac gctccggaga
     2521 atttccatcc cgttgctcga atcggcttcg acgaatatac tcagccaaca ccagattaag
     2581 agaggaagag agattggagt ggattgtcga aacagcaatc aacgaatgag acgaagccgg
     2641 agaggctatc actggggatt tttggtcgcg gcactcacca gcgcttttgc ggctaacgct
     2701 tggggcctgg acagggttcg gatcggcatg ggcgcattca gcccgaccaa cagcgcgatc
     2761 tgggtcgcag aggagcgcgg ctttttcaaa aagcatggga tcgaagctga agcgatcttc
     2821 atcggcggcg gtgcggcgcg cggcgtcaat gcattgctag ccggcgatat tcagttcgcc
     2881 accgcgggcg gcggagccgt catcacctcg gcgcttaagg gagccgaggt cgtcatggtc
     2941 gcagcgcaga acaataaagg agttcatcgg attctggtgc ggcccgaaat cgacacgccg
     3001 gaagaaatca aaggcaagaa aattgccatc acgacgttcg gttcttcttc ccacgccgtg
     3061 cttacgatgg tgctcgatgt atggaagatc cgccccgacg agttacaaat cttgcaagtc
     3121 ggctcatcac caaccatgct gatcagcttg caaaagaaat tggtcgatgg cgcggtgctg
     3181 acggatccat cctactttat tgccgaggat cacggttttc gggtgatcgc cgatctcgcc
     3241 aagatgaaca ttcactatct tcagagcatg atcgtatcga cgcgaagcta cctgcgggca
     3301 aatcgcgatc aggccacgcg agttctcaaa gcctacgtcg aaggaattgc ctacttcaag
     3361 aaaaacaaaa aagaaagcgt ccgcatcatc ctaaagaaaa tgcgcatgga tgcggaccaa
     3421 gcgaaatacg ccgagcgcac tta
//
LOCUS       DASUTG010000250         8543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_188364, whole genome shotgun sequence.
ACCESSION   DASUTG010000250 DASUTG010000000
VERSION     DASUTG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188364"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF089_05655"
     CDS             complement(<1..505)
                     /locus_tag="VF089_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173480.1"
                     /translation="MPKPKSLANPRSFARTTPSGFLQGLWEFLPAGESLPAMVWQTRH
                     RFLLRLTWLHALLIALIAVVFGNSWEFSLAALVRNGTVLRTIAEAIIVGMFASVAALT
                     TGRALPPLAVGFGLISASAFLVHLSGGAIEFHFHFFVVLALLALYQDRSSYVLVLVYL
                     VIYHAVIG"
     gene            complement(604..834)
                     /locus_tag="VF089_05660"
     CDS             complement(604..834)
                     /locus_tag="VF089_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6173481.1"
                     /translation="MDQKFESIRLLTLEEAAALLQVSKRTLQRMIKINELPAFKVGGQ
                     WRLREAQLRQWVEHRELSGTEREKKGDFGENR"
     gene            complement(1151..3079)
                     /locus_tag="VF089_05665"
     CDS             complement(1151..3079)
                     /locus_tag="VF089_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX6173482.1"
                     /translation="MARKRYLKKTPLKEAKELFLAKVDSSRIGNESIAVEDALDRVTA
                     QPVFAKISSPHYHAAAMDGICVRAEDTFGATEFNPKTFIRGSSESPAQNAFAYVDTGN
                     SLPAWANAVIMIEKVRQLKDGSVEIFESVGPWHHVRLVGEDIVATELLLPRYHRLRPY
                     DLGALLASGHTLVEVKSRPKVGIIPTGDELIAPGEQPRAGAVIEFNSTVLAAFVREWG
                     GTPIKYPRAKDQSELLEQAVRCAAAECDLVTIIAGSSAGEHDLTADVVADLGELWAHG
                     IDVMPGKPAVLGGVAGKPVIGVPGYPVSAIVIAREILRPALEKFLGARAPSYPSVHAV
                     VPKKIASHLGLEEFIRVSLGQVGGKLVAVPLARGAGVITTMVHADGLLRVPSLVEGLN
                     EGEEVEIELLRPREDIENTILCTGSHDLAIGVIEDQLKKHNPALKIAATNVGSVGGLL
                     ALQRGETHMAGTHLLDPETGVYNIPDIKRTIPKVPVVLVHLAQREQGIVVARGNPKTI
                     GSLCDLTSGIRFVNRQAGSGTRVLLDFELKRLGINPNSIDGYEREEFTHMSVGVAIAS
                     GLADAGLAVRAAAQALGLDFIAVASEQYDLVLSRSFFESECGERLMLVLQSAAFKDAV
                     AALGGYDSSDSGKLLYQQ"
     gene            complement(3180..4418)
                     /gene="glp"
                     /locus_tag="VF089_05670"
     CDS             complement(3180..4418)
                     /gene="glp"
                     /locus_tag="VF089_05670"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX6173483.1"
                     /translation="MAQTFFKVLAPEDALRLLVEVKPVATEQAATLAARGRVLSEDLH
                     SAVDLPHFSRAAMDGYAVRSKDTFGASQSLPAYLKLAGVVDMGKEAIQPVQAGEAIRI
                     STGGMMPPGSDAVVMVEYTNEVEGGLVEVHRGVSPAQNVIQVGDDIKKGELVFRSGRR
                     LKAHDIGALTGIGISSVPVFKQPRVALISTGDEIVDADVNPLPGQVRNINQHSLAGLI
                     AECGAELKDFGVIPDERQRLTTALERSLDWADLVLLSGGSSMGAKDIALETILSFPDA
                     EFIFHGISVAPGKPTIFARACGKPIMGLPGYPVSALVIFDLFAAPLIRRLGGEVIATH
                     SRFARTAKATLKTNIASQVGREDYVRVALEQETEGLLATPLPNKSGAIFTLVKADGMV
                     RIDLNQDGLEAGEEVEVILF"
     gene            4725..5318
                     /locus_tag="VF089_05675"
     CDS             4725..5318
                     /locus_tag="VF089_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX6173484.1"
                     /translation="MAGKNTAAFGIYPNESALENGISALQQAGYRNEDISVLFPENEG
                     TKDFAHRKGTKAPEGAASGAGTGAVAGGVLGWLAGIGAIAIPGVGPFIAAGPIMAALA
                     GAGVGGAVGGLTGALVGAGIPEYEAKRYEGRIKDGGILVSVHCDNSEWTSRAKDILER
                     TGAQDVSSAGEASADFAETDKPLPRDASGTVRRRDDV"
     gene            5775..6377
                     /locus_tag="VF089_05680"
     CDS             5775..6377
                     /locus_tag="VF089_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6173485.1"
                     /translation="MEPPVLEGLSPLWARNSCMHCEFAACAAVCPVEAITKYEEGPVV
                     INQEVCIGCEYCIHACPWEVITKNDITQKASKCTMCSDRISEDKQPFCVQACPTQALD
                     FGLTEEITAKANQQAEAVGGYTYGQKEAGGGNVIYVLKENKEKYGLRNISEQKFPKHK
                     IPLPLMLKDLFTPRCGIKGKLRALYLAIIHPKRLMYRYFS"
     gene            6447..7463
                     /locus_tag="VF089_05685"
     CDS             6447..7463
                     /locus_tag="VF089_05685"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173486.1"
                     /translation="MSPSTFSRSSIAYKWILTAVLMIPFTSEVWAEKIRTAIPQANLN
                     YLSIYVAEARGFFRDEGLENETVVISGPLATAALLSGDVDFSGGGGSGMRAALKGAPV
                     KGIYFQTEKVTFYLIADPSIKTAADLKGKKVAIGSSGDTQDRMITMFAERGGVSSRDI
                     TRIAMGSDTNSRILALKSGAAHASAVDPGGLVFAQKEGLVSLGFLGDLFPMPFQGFVT
                     VDRKIRENPGQIKRWLKAAIRGLMFVRDQSEEAVDLGIKKLQLGKATRPMIVEGTKNY
                     ARALPQGVPGLPTAEGIRNFLQYDIKIPLQIKEDIPPERLLHLQLVEEVKKELEAVQR
                     RPSR"
     gene            7460..8188
                     /locus_tag="VF089_05690"
     CDS             7460..8188
                     /locus_tag="VF089_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6173487.1"
                     /translation="MTPEKDLREQLATCTRIFAMQGLIGLFGHVSVYQPESKTIFITP
                     GSGSDKPHFQANDMIPIHLDGKPLDGKTLPPVEWPIHTALHGARTDALAVAHLHTPYA
                     TLFSIARREFHPVTLQGTIFGEGVPLYKEAHLVTTTQRGDNLLNTMGAHRAMLMRGHG
                     IVVAGKSLAEVLFASMILEDDAKKTMQALTLGPVDHFSAEECRGFNAEIDFERRSKRA
                     WEFFTRLESTWDRQGGSGRMELFP"
     gene            8342..>8543
                     /locus_tag="VF089_05695"
     CDS             8342..>8543
                     /locus_tag="VF089_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173488.1"
                     /translation="MSEGVRLYAGTQAGLIIWRSGTNGWQEIGRHFEDRIIDSISGCK
                     TMPERVFVGVTHDGLYRTIDGGE"
BASE COUNT         2084 a         2331 c         2279 g         1849 t
ORIGIN      
        1 caccgataac agcgtggtaa atcaccaggt aaacaagaac caggacgtac gaactccgat
       61 cctggtacag cgccaacaag gcaagcacaa caaagaaatg aaaatgaaat tcgatggcgc
      121 cgccggagag atgaacgaga aatgccgagg cgcttataag cccaaacccg accgctaaag
      181 gcggaagagc gcgaccggta gttagagcgg caacgcttgc aaacattccc acgattatcg
      241 cttcggcgat tgtccgaagc accgtgccat tgcgaacgag ggctgcgagg ctaaattccc
      301 aactattacc gaacacgacc gcgatcaacg cgatcagcaa agcgtgaagc cacgtaagtc
      361 gtaataagaa tcgatgccgg gtttgccaga ccatggccgg caatgattct cccgcaggca
      421 gaaactccca caagccctgc aggaatccgg agggtgtcgt tcgggcaaag cttctggggt
      481 tggccaagct cttaggtttg ggcatatcca tttctctgca actcagttgt ccggcggctt
      541 atctgaaagc gaaaaatcaa cgacagaatc ttcaccctga ggaaggccga gtgtgatcat
      601 ctattaacgg ttctctccaa aatcgccttt cttttcccgc tcagtcccgc tcaattctct
      661 atgttcaacc cattgcctca gttgagcctc ccgcagccgc cattgaccgc ccaccttaaa
      721 ggccggcagc tcattgattt tgatcattcg ctggagagtg cgtttggata cctggagcag
      781 tgccgcagct tcctcgagcg tcagtaaacg gatactctcg aacttttggt ccatagaact
      841 caaaaactcc cggcgagcgc tgacgcaacg gcaatgccat tgctcgaaat tgctaagtac
      901 ttataaaccc aagggatttg agaccatgaa caagacttgg aggagtggga tggacaaatc
      961 tggacaatcg gcgacgattg acacgggcat cgtcagtagt cagtaaaggg ggcttttcta
     1021 gagccgatag ttggcaggaa caacgttctc tgtgggccga tgcgccgtgt gaacgccgga
     1081 cggatttcga gtgtgccagt ccacatcacg atcgatctct gcgaactgac ctataggccc
     1141 tataactata ctactgttgg tagagtagct taccggaatc gctactatcg tacccgccca
     1201 aagcggccac agcgtctttg aatgctgcgg attgaagcac cagcatcagc cgctcgccac
     1261 attcggattc aaagaaggag cgcgacaaca ccaaatcgta ctgctcactg gcgacagcta
     1321 taaaatccag tcccaacgct tgtgcagcag ctcgcacggc gaggccagcg tccgctaaac
     1381 cactggcgat cgcaaccccg acagacatat gcgtgaactc ttcccgctca taaccatcta
     1441 tcgaatttgg attgatgccc aatctcttaa gttcaaaatc taacagcacg cgagtcccgg
     1501 atcccgcttg ccggttgacg aatcgaattc cggatgtgag atcgcacaag ctgccgattg
     1561 ttttaggatt acctcgagca acgacaatcc cttgttcgcg ctgcgcaagg tgtacaagta
     1621 ctaccggcac tttcgggatc gttcgtttaa tatccggaat attgtacaca ccggtctccg
     1681 gatcgagcag atgtgtgccc gccatgtggg tctcgccgcg ctgcagcgca agcaaaccgc
     1741 caacgctgcc gacattggta gccgcgatct tgagcgcggg attatgtttt ttgagttgat
     1801 cttcgatcac accgatggcg agatcgtgac tgccggtgca tagaatcgta ttttcgatat
     1861 cttctcgggg gcgaagcaac tcaatctcaa cttcttcacc ttcgtttaat ccttcgacga
     1921 gacttggaac ccgtaataat ccatcggcat gaaccatggt tgtaatcacc cctgcgcctc
     1981 tcgcaagggg gaccgccact agcttcccgc ccacctgccc gagcgatact cgtatgaatt
     2041 cttcaagacc gagatgggaa gcgattttct tcggaaccac agcatgcaca ctaggataag
     2101 atggcgccct ggctccaaga aatttctcca gcgccggccg caatatttcc cgtgcgatga
     2161 cgatggcaga gacagggtag ccgggaacac cgatgacggg cttgccggcg actcctccca
     2221 ggaccgccgg tttgcccggc ataacatcga ttccgtgagc ccagagttcg cccaaatccg
     2281 ccaccacgtc ggccgtcaag tcatgttccc ccgcggagga acccgcgatg atggtcacca
     2341 gatcacattc tgcggcggcg caccgcacag cttgttcgag cagctcggat tggtccttgg
     2401 cgcgaggata tttgatcggc gttccacccc attcgcgcac aaacgccgcg aggactgtcg
     2461 aattaaactc tataacggct cctgcccgcg gttgctcgcc cggggcgatc aactcatcac
     2521 cggttggaat gatcccgacc ttgggccggg atttcacctc tacaagggta tggccggaag
     2581 ccagcaaagc gccaagatca tagggacgaa gccggtgata gcgcggcaaa agcagttcgg
     2641 tcgcgacaat atcttcgcca accaaacgga catgatgcca gggtccgacg gattcaaaga
     2701 tctctacgga tccatctttg agctgacgga ctttctcgat catgattacc gcattcgccc
     2761 atgccggtag cgagttgccg gtatcaacgt aagcaaacgc gttctgagcc ggcgactcgc
     2821 tgctccctcg aatgaatgtt ttggggttga attcggtagc gccaaaagta tcttcagcgc
     2881 gcacgcagat gccatccata gcggcggcat ggtaatgggg agaagaaatc ttggcgaaga
     2941 ctggctgcgc ggtaacgcgg tcaagcgcgt cttctaccgc aatagactca ttgccaatgc
     3001 gcgacgaatc gactttggcc aggaagagtt ctttggcttc ctttaacgga gttttcttaa
     3061 gataccgttt gcgagccatc actctcctgg cttattaacc gttgcaaggg attctttctc
     3121 ggaatttcgg ctggggatta acacccgcaa ctgctcacac acggcgacgg tcctaggagc
     3181 tagaacaaaa tcacctcgac ttcttctccc gcctccagac cgtcttgatt gagatcgatc
     3241 cgcaccatac cgtcggcttt cacgagggta aaaattgcgc cggatttgtt cggtagcgga
     3301 gttgccaaaa gcccttcagt ttcttgttcg agagctacac gaacgtaatc ttcacggccg
     3361 acctgggaag cgatgttggt cttcaatgtt gctttggccg ttctagcgaa tcgagagtgg
     3421 gtcgcaatca cctcgccgcc caatctgcgg atcagaggcg cggcgaacag atcgaatatc
     3481 accaaagcgg agaccggata accgggaagt cccatgatcg gcttaccgca cgccctggca
     3541 aagatcgtcg gtttaccggg agccaccgaa ataccatgaa aaataaactc cgcatccgga
     3601 aacgaaagga tagtttcaag ggcaatatct ttggctccca tcgagctgcc cccggacagc
     3661 aacacgaggt ccgcccaatc caaagatcgc tcaagcgcgg ttgtgagccg ctgccgctca
     3721 tcaggaataa ctccgaaatc tttcaattct gcgccgcatt cggcaatcag accggccagt
     3781 gagtgctgat tgatgttgcg cacctgcccc ggcagcgggt tcacatcggc atctacgatc
     3841 tcatcacccg tggaaataag cgcgacacgc ggttgcttga acacaggcac cgatgaaatc
     3901 ccgatgcctg tcaatgcgcc tatatcgtgc gctttgagcc gcctgccgga tcgaaaaacc
     3961 aactcgccct ttttgatgtc atcaccgact tgaatgacat tctgcgccgg cgacacaccg
     4021 cgatgaactt ccaccagccc gccttcaacc tcgttagtat actcgaccat cacgacggca
     4081 tcgctccccg gcggcatcat gccaccggtt gaaatgcgaa tcgcttcgcc cgcctgcacc
     4141 ggctggatcg cctctttacc catatccaca accccagcca gttttaaata ggctggcagg
     4201 ctctgactcg cgccgaaggt atctttggaa cgaacagcat agccatccat tgcggcgcga
     4261 ctaaagtgcg gcaagtcaac ggcagaatga agatcctcgg agagcacccg accgcgcgct
     4321 gccaacgttg cagcctgctc ggtagctacg ggtttcacct caaccagcaa acgtaacgcg
     4381 tcttccgggg ctagcacttt gaaaaatgtc tgggccatag atgccaaatc ataatcagag
     4441 atagaacagg aagcaacgag gcctctagct ttcgcgttgg agcatgcagc tatataccag
     4501 gaaaggcgca tgggatgttg ccgatctcac cgtcagaatc attattcttg gattgcgccc
     4561 tgaggtgctg tcccagtcct gggctcgatc cccgcttctg attcccctgt ttgggacagg
     4621 gtgttataaa ttcgtgccat tgcgtgcacg ttagggatcc gccggttggc acgtgattcg
     4681 ctttttagtt cggccaaagc gaaactagaa aggagtaaat tattatggca gggaaaaaca
     4741 cggcagcatt cggaatatat ccgaatgaat cagctctcga gaatggaata agcgctctac
     4801 aacaggcagg ttatcgcaac gaagacattt cggttttatt cccggagaat gaaggcacca
     4861 aagatttcgc gcatcgaaag ggcacgaaag cacctgaagg cgcggcgagt ggcgcgggta
     4921 caggagcagt agccggagga gtcttggggt ggttagctgg cataggcgcg atcgcaattc
     4981 cgggtgtcgg tccgttcatt gcagcgggtc caatcatggc tgcactcgcc ggcgcgggtg
     5041 tgggcggggc tgtgggcggc ttaaccggtg ctctggtcgg cgcgggtata cccgagtacg
     5101 aggccaagag atatgagggc agaatcaagg acggcgggat tttagtctcg gttcattgtg
     5161 acaattctga atggacgagc cgcgctaaag acatattgga gagaaccgga gctcaggatg
     5221 tatcttcggc aggcgaggcc agcgccgatt tcgcggaaac cgacaagccc ttgccgcgcg
     5281 atgcctctgg cacggttcgc cgccgtgatg atgtttaaat agttcgataa atgtaatttc
     5341 tctttcgagt cacgccaacc gattcggctt gtcgagagtg cgcgcaacgc cgtgctccgg
     5401 caggccgaat cgcactgatt tgttcacttg tgcctttcta gtagccgctt ctaactactc
     5461 ccctccattt agcattcaga tcgacgttcg taatagtctc cagcgttttt gtacaccaac
     5521 caatagttga cgactgcaaa aaagaagtga tacatcgggg catgcgcgat aacgtgaaag
     5581 ccccgaccga cagtcttatc caacagtgcg gcagcctggt tccaaccgcg aggctccggg
     5641 atggtagagc aagaagcatt ctcttcgatg cgaccaaatg catcggctgc cggcactgtg
     5701 tccaggcttg taaggactgg aacgatcacg accgcaccag cctctatact ctctccagca
     5761 caaactggat aacaatggag ccgcctgtgc tcgaggggct ctcgccgctg tgggcacgaa
     5821 acagttgtat gcattgcgag ttcgccgcct gtgcggcggt ttgcccggtg gaagcgatca
     5881 cgaagtatga agaaggcccg gtagtcatca accaggaagt atgcatcggt tgcgaatact
     5941 gcattcacgc ttgtccgtgg gaagtcatta caaagaatga catcacgcag aaagccagca
     6001 aatgcaccat gtgcagcgac cgaatcagcg aagacaagca acccttttgt gttcaagcct
     6061 gccccaccca agcattggac ttcggtttaa ccgaagagat taccgcaaaa gcaaaccagc
     6121 aagccgaagc cgtgggcggt tacacgtatg gccagaaaga agctggcggc ggcaacgtga
     6181 tttacgtgct caaagaaaat aaagaaaaat acgggcttcg caatatcagc gagcaaaaat
     6241 ttccaaagca caagataccg ctcccattga tgctgaagga tttatttaca ccgcgttgcg
     6301 gtatcaaggg caagttgcgc gctctctatt tggctatcat ccatcccaag cggctgatgt
     6361 atcgttattt ctcgtgagcg ttacaacaat cgatgccgag cccgcccgct aaattcaaaa
     6421 agcgcaaaca tagggaccaa tcaaacatga gcccatccac cttcagccgt tcttccatcg
     6481 cgtacaaatg gattctcacg gctgtattaa tgatcccatt tacgtccgag gtttgggcag
     6541 aaaaaataag aaccgccatt ccccaggcca atctaaacta tctttccatt tacgtagctg
     6601 aggcgagggg cttcttccgc gatgagggcc ttgagaacga aacagtcgtg atctcgggac
     6661 cgcttgccac tgcggcgctc ttgagcggcg atgtcgactt tagcggcggg ggcggcagcg
     6721 gcatgcgggc ggcgctcaag ggcgcgccgg tcaagggcat ttattttcaa acggaaaaag
     6781 tgacgtttta cctgattgcg gatccaagca ttaagacggc cgcagacctg aagggcaaaa
     6841 aggttgcgat cggcagttca ggcgataccc aggaccgcat gatcacgatg tttgcggagc
     6901 gcggcggcgt ttccagcaga gatattaccc gcatcgccat gggctcggat accaacagcc
     6961 gaatcctcgc actcaagtcc ggcgccgcgc atgccagcgc cgtggatccg ggcggtctgg
     7021 tgtttgcgca aaaagaggga ctggtttcgt tgggttttct cggtgatctc tttcccatgc
     7081 cgtttcaagg gtttgttact gtcgatcgaa agattcgcga aaatccagga cagatcaagc
     7141 gctggctcaa agcagcgatt cgcggcctta tgttcgtgcg cgaccaatct gaagaagcgg
     7201 tagatctcgg catcaaaaag cttcaattag gcaaagcgac tcgtccgatg atcgttgaag
     7261 gaaccaaaaa ctatgctcgt gcattaccgc agggcgttcc ggggcttcct acggctgaag
     7321 ggataagaaa ctttcttcaa tatgacatca agatcccgct gcagatcaaa gaagatattc
     7381 ctccggaacg tttgttacat ttgcagctcg ttgaagaagt gaagaaagaa ctggaagccg
     7441 tacaaaggag acccagtcga tgacaccgga gaaagactta cgggagcagc ttgccacctg
     7501 cacgcggatc ttcgcgatgc agggcctaat cggcttgttc ggccatgtca gcgtttatca
     7561 acccgagagc aaaaccattt ttatcacgcc cggatcagga tcggacaagc cgcattttca
     7621 ggcaaacgat atgatcccga ttcaccttga tggcaaacca ttggatggta agactctgcc
     7681 tcctgtcgaa tggcctattc atacggcgct gcatggggct cgtacggatg cgttggcagt
     7741 ggctcatctc catacccctt atgcgacatt gttcagcata gcccgtcgtg agtttcatcc
     7801 ggtcaccttg caaggaacga tcttcggcga aggtgtgccg ctctataaag aagctcatct
     7861 ggtgacgaca acccaacgcg gcgacaatct gctgaatacc atgggcgccc atcgcgccat
     7921 gcttatgcgc gggcatggca ttgtcgtcgc cggcaagagt ctcgccgagg ttttgtttgc
     7981 atcgatgatt ttagaagacg acgccaaaaa gactatgcag gcgttgaccc tgggtcccgt
     8041 cgatcatttc agcgccgagg aatgccgtgg ctttaatgcc gaaattgact tcgaacgccg
     8101 ttccaagcgg gcttgggaat ttttcacacg cttggaatcc acctgggatc ggcaaggcgg
     8161 aagcggaagg atggagcttt ttccgtgaaa agcgtagtcg aaagctcaag cggtttaact
     8221 cgtcaggttc gaatgaggcg attgaacatt tggaacgacc ggaatgatgc cccgcttttg
     8281 aatcgatatt tctaagaggc tgcggccgtg aagtatcacg gatgctcaaa ggagaggagt
     8341 tatgagcgaa ggagttcgtc tttacgcggg aacgcaggct ggcctgataa tttggcgatc
     8401 cggcaccaac ggctggcagg agatcggtcg ccattttgag gatcgcatca ttgattccat
     8461 ttccggctgt aagaccatgc ccgagcgagt cttcgtcggc gtgacccatg atgggctgta
     8521 tcgaacgatc gatggcggcg aga
//
LOCUS       DASUTG010000251         4511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1670920, whole genome shotgun sequence.
ACCESSION   DASUTG010000251 DASUTG010000000
VERSION     DASUTG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670920"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VF089_05700"
     CDS             complement(<1..1256)
                     /locus_tag="VF089_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6173489.1"
                     /translation="MVQRTITVDGNEAAASVAHRVSEVIAIYPITPSSPMGELSDEWS
                     NQGRRNLWGNIPEVIEMQSEAGAAGAVHGALQAGALATTFTASQGLLLMIPNMFKIAG
                     ELTPFTMHVAARTVATHALSIFGDHSDIMACRPTGFAMLGAASVQEAHDFAAIAHAAS
                     LESRVPFLHFFDGFRTSHEVSKIYELSDEDLRALLPEAAIAAHRARGLTPDRPVLRGT
                     AQNPDSFFQAREAANLFYRACPVIVEETMTRFAARTGRRYGLFEYVGHPDAERVLVIM
                     GSGAETAHETVEFLAARGDKVGVLKVRLFRPFSIRGFLNVLPQSVRAIAVMDRTKEPG
                     ALGDPLYQDVVTALTEARAEGGSPFAAEPRVVGGRYGLSSKEFTPAMVQAVFDNLAQS
                     CPKNHFTIGIVDDVTQTSLSYDETFDT"
     gene            2386..3756
                     /locus_tag="VF089_05705"
     CDS             2386..3756
                     /locus_tag="VF089_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX6173490.1"
                     /translation="MSSAKATRAYRMALLCFSLLISLNLSESAFAQKTPIVYVAPIEG
                     MIDLGLAPFVDRVLREATDAGAAAVILEINTFGGRVDAAVVIRDALLNSRVSTVAFVN
                     KRAISAGALIALATEKIVMGDGGTIGAATPVQMGQPGAPAQPVEEKTVSYVRKEFRAT
                     AEARKRPPLIAEAMVDADVEIPDLIEKGKLLTLTTDEALKHKIAELRADTMAEVLNQL
                     ELAGAEVRRASPNWAENFVRFLTHPIVSSLLITVGMLGVILEIRSPGFGVPGIIGIGS
                     LALFFWGHWLVQLAGWEEILLVGSGLVLLMAEIFVIPGFGIAGVLGIAALLSGLSLSL
                     IGGGATWDFILKAVGRVIFSLLLALIGSLVLLRFLPRLPFGKRLILQTGLAAGEGYAS
                     PPETDRNWLGKTGTTVSPLRPSGIADVEGERVDVVSDGEFIEAGAQIVVSRVDGNRIV
                     VRRHRA"
     gene            3794..>4511
                     /locus_tag="VF089_05710"
     CDS             3794..>4511
                     /locus_tag="VF089_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HEX6173491.1"
                     /translation="MAGGFVVLLALLVAIVFVLYLVPIPLWIAAWASGAYVGLLTLIG
                     MRLRRVPPGTVVTARISAVKAGLNIPLNDLEAHFLAGGNVVRLVNAMISADKANIPLP
                     FKRAAAIDLAGRDVLEAVKMSVIPKVIETPKIAAVAKDGIQLIAVSRVTVRTNIDRLV
                     GGAGEETVIARVGEGMVSTIGSAASHKAVLENPDHISKNILSRGLDAGTAYEILSIDI
                     ADVDVGQNIGAKLQIDQADAD"
BASE COUNT          915 a         1268 c         1365 g          963 t
ORIGIN      
        1 gtgtcgaacg tctcatcgta cgatagcgaa gtctgggtca catcgtccac gataccgatc
       61 gtgaagtggt tcttcggaca agactgcgcg aggttatcga aaaccgcctg caccatggcc
      121 ggcgtgaact ctttggaaga caggccgtag cgaccgccca cgacgcgcgg ctcggccgca
      181 aacggcgagc ctccctccgc gcgggcctct gtgagtgcag tgaccacatc ttgataaagc
      241 ggatcgccga gcgcaccggg ttctttggtg cggtccatga ccgcgattgc gcgaaccgac
      301 tgggggagga cgttcaagaa cccgcggatc gagaatggcc gaaacaggcg caccttcaac
      361 actccgacct tgtcgccgcg tgccgctaga aactccacgg tctcgtgcgc agtctcggcg
      421 ccggacccca tgatcacgag cactcgttcg gcgtcggggt gaccgacgta ctcgaacaag
      481 ccgtaccttc ggcccgtgcg cgcagcgaat cgcgtcatcg tctcctcaac gatcaccggg
      541 caggcgcgat agaatagatt tgccgcttcg cgcgcctgga agaacgaatc aggattttgc
      601 gcggtgccgc gcagtacggg ccggtccggc gtcagtcccc gtgcccgatg cgcggcgatc
      661 gctgcttccg gcagcagggc ccgtaaatct tcgtcgctca gctcataaat ctttgagacc
      721 tcgtgcgaag ttcgaaagcc gtcgaaaaag tgcaggaatg gcacgcgcga ttcgagcgag
      781 gccgcatgag cgatggccgc gaagtcgtgc gcctcttgaa ccgaggcagc gccgagcatc
      841 gcgaatccgg tcggccggca cgccatgatg tcggaatggt cgccgaagat cgacagcgca
      901 tgagtggcga ccgttcgcgc cgccacgtgc atcgtaaagg gggtcagctc cccggcaatc
      961 ttaaacatat tggggatcat cagcagcaaa ccttgcgatg ccgtgaaggt cgtggccagc
     1021 gcgcccgcct gcagcgcacc gtgcaccgcg cccgccgcac ctgcttccga ctgcatctcg
     1081 atcacctccg ggatattgcc ccagaggttc cggcggcctt gattcgacca ttcatcggac
     1141 agttcgccca tgggtgagga aggcgtgata ggataaatgg cgatgacttc gctcacgcgg
     1201 tgcgccactg acgcggcggc ttcgttgccg tcgaccgtaa tcgttcgctg gaccataaaa
     1261 tcttccccca aaaaaaatta gctcagtgcc gatcaaccgt gatgtgtggc gaatgacata
     1321 cgggcatgcg gttggtgcgc atgatccttc cggctcctgt gaaatcggcc tccctggcct
     1381 gttcatcaat catcctcgaa atctttttaa ttttttggtt ccgcatagac atcatctgtg
     1441 tgccgatcga gcgctgagct tggagaagcg aaggcgcaga aacgagctat ttcactgctg
     1501 tatagggttg cgtggtcaaa ggctgcttat tctccggaag tccgatgaag taaccgagat
     1561 tgcaggccgc cctcaaggca caggtccatt ctttctgcgg agggttgcgc ttgatcgtgt
     1621 gtcactttcc cggcatgtca cttgtccgtt gtaggttgct ctttgttact gctgttgcgc
     1681 cgagactcga cgccatcggc cattcacgtc cgcaccccca gacgaggcag cacccagcgc
     1741 atcaacacga tataggctac gaagaacgca atgaaccaca accagtcagg cataaatttg
     1801 atctcctttg ttacggaatg ttagcggact gagtgctgcg atgtcttggc ggcgctcttc
     1861 gtctcttcgt cgttgaaggc tgattcttga agacgctgga ctgcaacgga taagctagct
     1921 ctctttcggg agttcaacaa gagcaaagtc gattcgcagt gtgagaagga tgctctggaa
     1981 aaatgcagcc gcaatgataa tgcaaggcga tcgtcgcttt cgtagcccac aacgttcccg
     2041 gagccggcgc agtcacctac gaattaccag aaccgtttcc cagcggaccg ggcgaggcca
     2101 gcacctgata gtgaaagagc tgaggctcgc ttttgacggc gctcttgacc gagttggaga
     2161 acgacacaaa ctcttcaccg tacgcttcca gagctgacgc tgacgtgcgg aaaactataa
     2221 gagcatggaa atccgagtga aaagactggt atggtgttct cggattgaaa tggcgcaagc
     2281 agccgctttc taaaaccgac atggcgccta cagaactaga tactcgtggt gtaggtcctg
     2341 tcccccggcg gcaggaactt tgaatgacaa tggacgaagc aaatcatgag cagcgctaaa
     2401 gcgacacgtg cctatagaat ggcgcttctt tgttttagct tattgatttc tttgaacctg
     2461 agtgagtcag ctttcgctca gaaaaccccg atagtttacg tcgcgcccat cgaggggatg
     2521 atcgatctcg ggctcgcgcc gtttgttgac cgcgtcctgc gcgaggccac cgatgcaggc
     2581 gcggcagcgg ttattctgga gatcaatact ttcggcggaa gggtggatgc ggcggttgtc
     2641 atacgcgatg cgctgctcaa cagccgggtg agcacggtgg cgttcgtcaa taagagagca
     2701 atctcggcag gcgcgcttat cgcgctggcg accgagaaga tcgtcatggg cgatggagga
     2761 accatcggcg cggcaactcc ggtgcaaatg gggcagccgg gcgcgccggc gcagccggtg
     2821 gaggaaaaaa ccgtttccta tgtgaggaaa gaatttcgcg ccaccgccga agcgcgcaag
     2881 cgcccgccgc taatcgccga agccatggtg gatgccgacg tggagatccc ggatctgatt
     2941 gaaaaaggaa aactcttgac gcttaccacg gacgaggctc tgaagcataa gattgccgag
     3001 cttcgcgccg atactatggc cgaggttttg aaccagctgg agcttgccgg ggccgaggtg
     3061 cggcgcgcat ctcccaactg ggcggagaac tttgtccgct ttcttaccca tccgattgtc
     3121 agctctcttc tcattaccgt cggtatgctg ggagttatcc tggaaattcg atcgcccggc
     3181 ttcggcgtgc cgggaattat cgggatcggc agcctggcgc ttttcttttg ggggcattgg
     3241 ttggttcagc tcgccggctg ggaggagata ttgctggtcg gttccggcct cgtgctgctc
     3301 atggccgaga tctttgtcat tcccggtttc ggtattgcag gagtcctcgg gatagcagcg
     3361 ctgctttcag gccttagctt gagcctcatt ggcggcggcg cgacgtggga ttttatactc
     3421 aaagccgtgg gtcgagttat tttttctctg ctgctcgcct tgattggaag tctagtgctg
     3481 ttgcggtttc tgccgcgatt gccattcggc aagcggctga tcctacagac cgggcttgcc
     3541 gcgggtgagg gctacgcttc gccgcccgag accgatcgca attggctggg gaaaaccggc
     3601 acaacggttt cgccgctgcg tccctccggg attgccgatg tcgaaggcga gcgagtggac
     3661 gtcgtctccg acggcgaatt tatcgaggcg ggagcgcaaa ttgtcgtgag tcgcgtcgat
     3721 ggtaaccgca tcgtggtacg gcgtcatcgc gcataactga aaggggtgaa aaatgttcgg
     3781 agctgagacc ggtatagcgg gaggattcgt cgtcctcttg gcgctgttgg tggcgatcgt
     3841 cttcgtgttg tatctggttc cgattccact gtggatcgcc gcctgggctt ccggcgcgta
     3901 cgtcggactt ctcactctca tcggcatgag actccggcgg gtaccaccgg gaaccgtggt
     3961 taccgcgcgc atcagcgcgg tcaaagccgg gctgaatatt ccgctcaacg atctcgaggc
     4021 gcattttctt gcgggcggca atgtcgtcag gctggtcaac gccatgatct cggcggataa
     4081 ggcgaacatt ccactgccgt tcaagcgcgc cgccgccatc gatctcgctg gccgcgatgt
     4141 tctcgaagcc gtcaagatgt cggttatacc aaaggtcatc gagacgccaa agattgccgc
     4201 ggttgctaaa gacggtatcc agctgattgc ggtctctcgc gtgacggttc ggacgaatat
     4261 cgaccggctg gtcggtggcg ccggcgagga aacggtcatc gcgcgggtcg gcgaaggcat
     4321 ggtcagcacc atcggatcgg ccgcatcgca caaggcggtg ctggaaaatc ccgatcacat
     4381 ttcgaagaac atcttgagca gaggtctcga tgccggaacg gcttacgaga tcctttccat
     4441 cgatatcgcc gacgttgacg tcggtcagaa catcggcgcc aagctgcaaa tcgaccaagc
     4501 cgatgccgat a
//
LOCUS       DASUTG010000252         4891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1226073, whole genome shotgun sequence.
ACCESSION   DASUTG010000252 DASUTG010000000
VERSION     DASUTG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226073"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF089_05715"
     CDS             <1..475
                     /locus_tag="VF089_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6173492.1"
                     /translation="ILGDGKLGNLCAQVLRLTGAKVTALGKHPDKLDLIKRTGVRTIL
                     LNDWQPRLFDIVVEATGSATGLELAISAVRPRGTLILKSTIAGAHQVSLAPVVINEIN
                     VLGSRCGVFPDALEALAAGHLSVAPLIEQVYPLEQGIEAIQHAGRRGARKILLRP"
     gene            complement(552..1154)
                     /locus_tag="VF089_05720"
     CDS             complement(552..1154)
                     /locus_tag="VF089_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX6173493.1"
                     /translation="METPHTIREYWFGTALEDAAVTARERAPLWWSKNPQLDDEIRRR
                     FESCVIKAGSGELDLWASNPQDRLALILLTDQFPRSIYRDSAKAFAFDTKALSLAREG
                     VDAGFDARLRPLEKVFFYLPLEHSESLADQQRSVRLFQKLLDQAGTDQKPTFAEYLDF
                     ALRHRDIISRFGRFPHRNGALGRISTPEELLFLQQPGSGF"
     gene            complement(1185..1484)
                     /locus_tag="VF089_05725"
     CDS             complement(1185..1484)
                     /locus_tag="VF089_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173494.1"
                     /translation="MAIPWFQIVKLVPEIVSLSKQLLQQTKTVQSTGEKPLEERVAEL
                     EMNEQKQAELVASMAQQLAAMTDALTALRKQVSVFRLFSIISLLLAIAAMIVALV"
     gene            1703..2779
                     /locus_tag="VF089_05730"
     CDS             1703..2779
                     /locus_tag="VF089_05730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6173495.1"
                     /translation="MSIDFVTNQEIILAARRNLTQDIWDYLSGGAESETTMRRNRIAF
                     DSLALCPRVCVDVSKIDTSTTFLGQTLRIPVMMAPIGSLQTITPEGGVAVAKAAAEFG
                     TMNFVSSVTQPSLEEIAASTKHPKIFQLYIRGGLDWCEEIIERVKRAGYMALCLTVDT
                     AHYSRRERQMMNRWLPPNKRSETTRHFQATLTWEMMAAIKKMAGLPFILKGVATAEDA
                     KIALDHGVDVIYISNHGGRQLDHGRGTMDVLPEIVQVARGKADIILDGSILRGTDVLK
                     ALALGCKAVTIGKLQGWGLGAGGQAGLVRVLELLEEELIIDMGLLGVTRIDQISERYV
                     CKAPPVCFPNEMSAFAHLPGGQVR"
     gene            complement(3042..3830)
                     /locus_tag="VF089_05735"
     CDS             complement(3042..3830)
                     /locus_tag="VF089_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173496.1"
                     /translation="MNKPREPRAGEKLTAQEAKAYVDYLYSTASKASQERIHNGHFMT
                     QLRAGTLPLAVIRQFFKNWGHFSMELNGLDAVSYYTHLPFFVRNFELLGPFCAKVADK
                     LISPKAPGQVLVLLQTAKELGLTRKEMLEEPYLPAARAINDFCHKVFIDGSIAELWGL
                     HVFEESLSRWMGEWYTALTTKYGLTGEQAIYFSTREEAGRESQNLRAGDDGLSGSGFH
                     RTVLERVLQDKIEFRAGYTIEYCALTMIELHAQMKQAAIDNPYP"
     gene            3950..4306
                     /gene="queF"
                     /locus_tag="VF089_05740"
     CDS             3950..4306
                     /gene="queF"
                     /locus_tag="VF089_05740"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX6173497.1"
                     /translation="MPSLPSKRLETFPNPHPERDYEISMEAPEFTCVCPRTGQPDFAT
                     IRIRYIPDRLCVELKSLKLYLWSYREEGAFHEAVINRILDDLVDACRPRKITVVGDFN
                     IRGGIHTTITAGYPSL"
     gene            4606..>4891
                     /locus_tag="VF089_05745"
     CDS             4606..>4891
                     /locus_tag="VF089_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6173498.1"
                     /translation="MAALEDNLRKVPPQNLEAESSVLGGILLENDAINVVLEVLRPDD
                     FYRESHRKIFRAMIELLDRNEPVDLITLSDFLKGRSELEAVGGTSYLASLA"
BASE COUNT         1109 a         1322 c         1322 g         1138 t
ORIGIN      
        1 aatcctcggt gatggcaaac tcggcaacct ctgcgcccag gtcctgcggc ttacgggcgc
       61 gaaggtgacg gcgctcggca aacacccaga caagctcgat ctcatcaagc ggacgggggt
      121 gcgcaccata ctgctaaacg attggcagcc gcgccttttc gatatcgtcg tcgaagccac
      181 tggatccgcg accgggctcg aactggcgat cagcgccgtg cgcccgcgcg gaacactcat
      241 tttgaaaagc accattgccg gagctcacca ggtatcgcta gcgcctgtgg tgatcaatga
      301 aatcaatgtt ctaggttcac gctgcggtgt ctttcctgat gccctggaag cgctcgccgc
      361 cggccaccta tcggtcgcac ctttaattga gcaagtttat ccgctcgagc aaggcattga
      421 ggcgatccaa cacgccggcc gccggggcgc gcgaaagatt ttgctccggc cgtaatctgc
      481 accggacccg gcaggagggt tggaggactg agtcttgaac ggggtgaggt gcgctggccg
      541 cgaatgagaa tctaaaatcc cgacccaggt tgttgcagga acaacagctc ctcaggtgtt
      601 gagatccgcc caagcgctcc gtttctatgc ggaaagcggc cgaagcggct gatgatatcc
      661 cggtggcgca aggcaaagtc gagatattct gcaaacgtgg gcttctgatc ggtaccggct
      721 tgatcgagca gtttctgaaa cagtcttacc gatcgctgtt gatctgcgag tgattccgaa
      781 tgctccaggg gcaggtagaa aaaaactttt tcgagagggc gcaatctagc atcaaagccg
      841 gcgtcaacgc cttcgcgagc aagagacaac gctttggtgt cgaaggcgaa agctttggct
      901 gaatcgcgat aaatactccg gggaaattga tctgtgagca gaatcagcgc gaggcggtct
      961 tgcggattcg aagcccagag gtctaactct cccgatccag ccttgatcac gcagctttca
     1021 aagcgccgcc gaatttcatc atcgagttgc gggtttttcg accaccacaa cggcgcccgc
     1081 tcgcgggccg tgacagcggc atcctccaag gcggtgccga accaatattc gcgaatggtg
     1141 tggggagtct ccatcatttc gtgaaacttg ttgatacgag aaacttaaac gagtgccact
     1201 atcattgctg ctatggctaa caataaggag attatcgaaa acagtcgaaa taccgaaacc
     1261 tgtttgcgca gagcggtaag ggcatcggtc atcgccgcca gttgttgggc catgctggcg
     1321 acgagctcgg cttgcttctg ttcattcatc tcgagctccg cgaccctctc ctcaagcggc
     1381 ttttccccgg tagattgtac cgttttggtc tgctgcagga gctgcttaga caacgacacg
     1441 atttcgggaa ccagtttaac gatttgaaac cagggtatcg ccattttctc tcgattcgtt
     1501 ttgggcttca gcggtctgtt acacgtaaac agcgccaatc taacatgtgg cggagcacat
     1561 cactatctct ctttgtcgcc ataaaggtcg cgtattctaa ctccagtcat ctcaggtttg
     1621 acagttgccc gaactgttag ataatctctc ggcgattacg ctacggcgtt gagaactcgc
     1681 aggactgaag aggagatctc tgatgagcat cgattttgtc actaaccagg aaatcattct
     1741 agcggcgcgg cgcaatctca cccaagacat ttgggattat ttgagcggcg gagccgagtc
     1801 tgaaacgacc atgaggcgca accgtatcgc cttcgatagt ctcgcgctat gcccgcgagt
     1861 ctgcgtggat gtctccaaaa tcgatacctc aacgactttc ctggggcaga cgctgcggat
     1921 tcccgtcatg atggcgccca tcggctcgct gcaaacgatc acccccgagg gcggtgttgc
     1981 ggttgctaaa gccgccgcgg agttcggcac gatgaatttt gtcagctcgg taacccagcc
     2041 gagtctggaa gaaatcgccg caagcacgaa gcaccccaag atattccagc tctacattcg
     2101 cggcggcctg gactggtgtg aggaaatcat cgagcgggtg aaacgggccg gatatatggc
     2161 gctgtgtctt accgtcgaca cggctcatta cagccggcgc gagcgccaga tgatgaatcg
     2221 ctggttgccg cccaacaaac gcagtgaaac cacccgccac ttccaagcga ccttgacctg
     2281 ggagatgatg gcggcaatta agaagatggc cgggctgccg tttattctca aaggcgttgc
     2341 tactgctgaa gatgccaaaa tcgcgctcga tcatggggtt gacgtgattt atatatcgaa
     2401 tcatggcggc cggcagctcg atcacggacg cggaaccatg gacgtgctgc cggagatcgt
     2461 ccaagtcgcg cgcggcaagg ccgacatcat tcttgatgga agtattttac gcggaacgga
     2521 tgttttaaaa gcgttggcgc ttggctgcaa agcagtaacg atcggcaaac ttcagggctg
     2581 gggtctaggt gcgggaggac aagccggcct ggtgcgcgtg ctcgagttgc tcgaagaaga
     2641 gcttatcatc gatatgggcc tgctcggcgt aacgcgaatc gatcagatct ccgaaagata
     2701 tgtgtgtaaa gcgccgccgg tatgtttccc caacgagatg agcgcgttcg ctcacctgcc
     2761 tggcggccaa gtgcgctagc tcgagcgcaa ccgaggctat tgcggcaagg agcaggatcc
     2821 gcgagagatc tccaatttgc gcgcggctca gttgagtagt acgtatgcga cgattgagag
     2881 ctgcttccgg aaacctttga caccaggttt agcggtgttt gtgaaccgtc atccacacct
     2941 aacttaattc cactggaatg cgagctgcga ttcgctatgc ggtcagcgtg attgctgcaa
     3001 caactcggcg gtcgccacgg cgacttaatc gcgaaatctc atcatgggta ggggttatct
     3061 atagcagctt gcttcatctg agcgtgcagt tcaatcatcg tcaacgcaca gtattcgatc
     3121 gtatagccgg cgcgaaattc tattttgtcc tgaaggacgc gctcgagcac tgtccgatga
     3181 aatccgctgc cgctgagtcc gtcgtcacct gcgcgtaggt tctgactctc cctgccggct
     3241 tcttcacggg tggaaaaata aatcgcctgc tcgccggtaa gcccgtactt tgtcgtcaaa
     3301 gcggtatacc actcgcccat ccagcgagac agggattctt cgaaaacatg caaaccccag
     3361 agctcggcga tggatccgtc tatgaaaacc ttgtggcaga agtcattgat cgcgcgcgcc
     3421 gccggcagat acggctcttc caacatttct ttccgggtga gccccaattc ttttgcggtc
     3481 tgcaatagca ccagcacctg tccgggcgct ttgggagaaa tcaatttgtc agcgaccttc
     3541 gcgcagaagg gtcccagcag ctcgaaattg cgaacgaaga aaggtagatg ggtgtagtat
     3601 gagaccgcat ccaggccgtt gagctccatg gagaaatgtc cccagttttt gaagaactgg
     3661 cggatcaccg ccagcggtaa agttccagct cgcagctgag tcatgaagtg cccattgtga
     3721 attctctcct gcgacgcttt cgaggcggtc gaatagagat aatcgacata ggcctttgct
     3781 tcttgcgcgg ttagcttttc acccgctcgc ggttctcgcg gcttgttcac gtgctacctc
     3841 ttcaaacagc tctgctgatc atgaaatgtc cgttcgtcat gcgactatca acagtatcaa
     3901 tatttctaat gaattggcaa agcctataac cgtgcgttag gatattcgta tgccgagtct
     3961 tccaagcaag cgattagaga catttccaaa cccgcatccg gagcgcgact atgagataag
     4021 tatggaagcc ccggagttta cttgcgtctg cccgcgcacc ggtcagccgg atttcgcgac
     4081 catccgaatc cgctacatac ccgatcgcct ttgcgtggag ctcaaatctc taaagctcta
     4141 tctctggtct tatcgcgaag aaggcgcatt ccacgaagcg gtcatcaacc gaatcctcga
     4201 tgatcttgtc gacgcatgcc ggccgcgaaa gatcaccgtc gtcggcgatt tcaatattcg
     4261 cggcggcatt cacacgacga tcacagccgg ttacccatcg ctctagcttc ttctcattgc
     4321 gcaactgttg ttgctgctac ccggtgcctg caaccaaaaa aatccactgc gttgcgcgct
     4381 gttgataagc ttgtgcacaa gctgttcacc gtgcacacaa ctagttgcct caaattcaga
     4441 atttccaagt tcttgctttt caacaatttt ttggcgcgga gattccagct cattaatcaa
     4501 gtaacgagac gaaattgttc ttgcttttta attgtggttg ggttcaaatt cagaaacatt
     4561 tttcacaata cagtatcgcg tcaggattct ctctcccaat ttttcatggc agctttagaa
     4621 gataacctac gaaaggttcc accgcagaat ctcgaagccg aatcctcggt tctaggggga
     4681 attctgctcg agaacgatgc catcaacgtg gtgctggaag ttttgcggcc cgacgatttt
     4741 taccgcgaat ctcatcgcaa gatctttcgg gctatgatcg agcttttgga ccgcaatgag
     4801 ccggtggacc tgatcacgct cagtgatttt ctcaaaggca gaagtgagct cgaagcggtg
     4861 ggcgggactt cttatctggc atcgctcgcg g
//
LOCUS       DASUTG010000253         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1967245, whole genome shotgun sequence.
ACCESSION   DASUTG010000253 DASUTG010000000
VERSION     DASUTG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967245"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VF089_05750"
     CDS             <1..414
                     /locus_tag="VF089_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6173499.1"
                     /translation="AVMVDESLVTLDQARRLIDLGACDYFNVRLSKNGGISGSLAIAR
                     LAQEAEIKIQVGAQVGETGILSAAGRTLAAHLPALAFAEGSFGTWLLAEDVTFENVAF
                     GSGGRAPLLQSRGLSVTVKEDVVERLATAKIDLRR"
     gene            421..2106
                     /locus_tag="VF089_05755"
     CDS             421..2106
                     /locus_tag="VF089_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEX6173500.1"
                     /translation="MRLLVKAAAFVTRRQWTRWHKLTSKPQETQDRLLLDLIGRHRST
                     RFGRDHRFESIRSLSDFRNQVRIADYERFRPYVELVKNGERDALTAEPIDMFTMTSGS
                     TGEPKLIPVTAKTRENHRQLTRLWYYRALVDHPDLFNGSLLGVVSPAVEGETAGGIPF
                     GAASGLIYRSSPRWIQNAYAVPYEVAEIKDFEARYYLIMRLALEQDISFFGTPNPSTI
                     LKLIQSASRNKYDIIKDIHDGTITSRFDLSAQLRASLASRLAKNLPRARRLESLIKHD
                     GALRPKEYWPRLQLIGCWKGGSAGVRLKEFAGWFGKTTPVRDLGYMASEAQMTLPVFD
                     SGSAGILAVDKNFYEFIPETEINASAPTILTCSELEEGNLYYIVLTTPGGLYRYDIND
                     VIRVAGFYNQTPLIEFVRKGRDVTSITGEKLHVNQLIGAMKRAQSAAGVAVQHFRACA
                     DVEKSLYVLSVELDGVMPSRQSLVQLLQELDAGLQELNVEYAQKRESGRLFAPVLCVM
                     KPGWFERKAQAILQGGGRDTQFKAQLLCVTPEDPDEIQLILDSPDAAPGRVPN"
     gene            2113..2553
                     /locus_tag="VF089_05760"
     CDS             2113..2553
                     /locus_tag="VF089_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173501.1"
                     /translation="MHGPALAILTFLFALRVLGQALVVFFRVDWLPGTEHWFSGVFRY
                     PVLLAIQLVMLPGMAKIASDVWRGRGFFAKPRPGCSPFLVACSIIYAISMVLRYALTM
                     AYRPEMRWFGGTIPIFFHFVLAAFVYTWGKFYSKSDTPSAQQHF"
     gene            2802..3794
                     /locus_tag="VF089_05765"
     CDS             2802..3794
                     /locus_tag="VF089_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6173502.1"
                     /translation="MASTPIFNGEQAITRLRDTVHGKAANFYAMYSSILGGIVKDPAV
                     MVLPLDDHMVHRGHGVFDTATLTHGMLYQLDAHLDRLIRSAQGARIPLPFPREELRRI
                     ILDTAAASRQREGSIRYWLSAGPGGYGLGPAECVGSSFYVIVFKQEAYPESYYRKGIK
                     IITSGVPIKPPLFARIKSTNYLPNVLVLLEAKDHGADNGIFIDERGMVAESSNMNVAF
                     VTEDRVFRHPPFETILPGITIQRVLEFARLLVQQDVLSAIDLAAVPVQKGRESAEMML
                     IGSSIKVAPVVEWDGQKIGDGKPGPIAKKLLEMWNEDAAAAQDQLVEVPYATIN"
     gene            complement(4094..>5128)
                     /locus_tag="VF089_05770"
     CDS             complement(4094..>5128)
                     /locus_tag="VF089_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173503.1"
                     /translation="AHGNEAHQIYDVRNPSAPVLRTTVVSGLEGTHKNWWECDTGIAY
                     LVSGVPGWRTDRMTQVFDLSNPRSPVHIRDYGLVGQEPGSTGEVPTDLHGPISAGHRV
                     YFGHGTSSNGTVQIVDRTKLLTGPPEPTPENLTFPVIAQFDTSPLVGAHTTFPILGVR
                     VPELGPFNVGGTRDILAIVNESTANECTGESHQMVFFADVTIESTPQVISNYHVPDPE
                     GVFCRRGGRFGAHSSNESFTPIYYGRILFVAYFNAGVRAVDIRNPFDPKEIAYFVPAT
                     TANTDPRAGKIAIQTNNVEVDDRALIYIVDRANTGLHILELTGPAREIANLPIQQQQI
                     EVQSRLTTRE"
BASE COUNT         1097 a         1463 c         1484 g         1084 t
ORIGIN      
        1 gcagtgatgg tcgatgagtc gctggtgacg ctggatcagg cacgccgcct gatagatctc
       61 ggcgcttgcg attatttcaa cgttagactg tccaagaacg gcggcataag cggcagccta
      121 gcgatcgcca gactggcgca ggaagcggag atcaaaattc aagtcggcgc ccaggtcggc
      181 gaaaccggca ttttgtccgc cgcaggacga actttggcgg cgcatctgcc ggcactcgcc
      241 tttgccgaag gctcattcgg tacctggctg ctcgccgagg atgtcacgtt tgaaaacgtc
      301 gcgtttggtt cgggcggccg ggcgccgctt ttgcaaagcc gcggcctgag cgtcacggta
      361 aaggaagacg tggtggaacg cctcgccacg gcgaagatcg atctgcgccg gtaaccatcg
      421 atgcgactac tcgtcaaagc cgcggcgttc gtaactcgcc gccagtggac acgctggcat
      481 aagctcacat ccaagcccca ggaaactcag gaccggctac ttctagatct catcgggcgt
      541 catcgctcta cgcgcttcgg ccgcgatcac cgtttcgaat cgattcgctc tctgagcgac
      601 tttcgtaatc aagtgcggat cgccgactac gagcggttcc gtccctatgt cgagttggtt
      661 aagaacggcg agcgcgacgc gctgactgct gaacccatcg acatgttcac gatgaccagt
      721 ggcagcaccg gtgagcccaa actgatcccg gtaacggcaa aaacgcgaga gaaccaccgg
      781 caattgacgc gcctgtggta ctaccgcgcg ctcgtggatc atcccgatct attcaacggc
      841 agcctgctcg gcgtcgtcag cccggcggtc gagggagaga cagccggcgg cattccattc
      901 ggcgccgctt cgggtttaat ttaccggagc agcccgcgtt ggatacagaa cgcttacgcc
      961 gtgccgtacg aggtcgccga gatcaaagac ttcgaagcca gatactatct gatcatgcgt
     1021 ctggcgctgg aacaggatat ctcgtttttc ggcacgccca atccgagtac gattctcaag
     1081 ctgatccaaa gcgcgagtcg aaataaatac gacatcatca aagacatcca tgacgggact
     1141 attacgtcgc gtttcgacct ttcggcgcaa ttgcgcgcat cgctcgcgtc acggctggcg
     1201 aaaaatctgc cgcgcgcgcg gcgactggaa tctttgatca agcacgacgg agcgctccgg
     1261 cccaaagaat actggccgcg cctgcaactt atcggttgct ggaaaggcgg cagcgctggc
     1321 gtgcgactga aagaattcgc cggctggttc ggcaaaacca cgcccgtgcg cgatctcggt
     1381 tacatggcca gtgaggcgca gatgaccttg ccggtattcg actcagggtc ggcgggcatt
     1441 ctcgcagtcg ataagaactt ctacgagttc atcccggaga ccgaaatcaa tgcgtctgcg
     1501 ccaacgattt tgacatgctc agagctcgag gagggaaatc tttactacat tgttcttacc
     1561 acacccggcg gattgtatcg ctacgacatc aacgacgtga tccgcgtcgc tggattttac
     1621 aatcaaacac cgctcatcga attcgtccgc aaaggccgcg atgttaccag catcaccggc
     1681 gagaagttgc acgtcaacca gctaatcggc gccatgaagc gggcgcagag tgccgccggc
     1741 gtggcggtgc aacattttcg cgcttgcgcc gatgtggaaa agtctctgta cgttttatcc
     1801 gtggaactgg acggcgtcat gccaagccgg caaagcctgg tgcaactgct gcaagaactt
     1861 gatgcgggcc tgcaagaact gaatgtggaa tacgcgcaaa aacgcgagtc ggggcggctc
     1921 ttcgcgccgg tcttgtgcgt gatgaagccc ggttggttcg agcgcaaagc tcaggcaatt
     1981 ctgcagggcg gcggccgcga cacgcaattc aaagcgcaac tcctgtgcgt cacaccggaa
     2041 gacccggacg aaatccagct aatcctcgac agcccggacg cagcaccagg cagagttcca
     2101 aattgacacg gcatgcacgg ccccgctctg gcgatcctga ctttcctctt tgcgcttcgc
     2161 gtattgggcc aggctctggt ggtttttttt cgagttgact ggctgcctgg cacagagcac
     2221 tggttctccg gtgtgttccg ctatcctgtt cttctcgcca tccagttggt tatgctaccc
     2281 gggatggcta aaatcgcatc cgacgtctgg cgcggtcgag gcttcttcgc caagccgaga
     2341 cccggctgtt ccccgttctt ggtcgcctgc agcattattt acgcgatcag catggtgctg
     2401 cgctacgctt tgacgatggc ctatcggccg gagatgcgct ggttcggcgg cacgattccg
     2461 atttttttcc acttcgtcct tgccgccttc gtgtacacct ggggtaagtt ctattctaag
     2521 agcgacacac cgagtgccca acagcatttc taaagaaatc atgcagcgca cgagaagagg
     2581 ttaggcttcg agtctctttc ctgctcctcg cgctgagatc acgatgcatg aacaagcaca
     2641 cgaagttgcc gattgcgcca cacgaccgtt tgacaacgca cgcggcgagt ggtgccgtga
     2701 gcctcttctg tgtatttgta gagtaaaatg gtctgtgatc actttggcag atccttacac
     2761 ttgacggcgg agaaaactca gagtgaagag gtgaaatgat catggctagc actcctatct
     2821 tcaacggcga gcaagcgatc acccgactgc gcgacacagt gcacggtaag gccgcgaact
     2881 tttacgcgat gtactcgagc atcctcggcg gcatcgtcaa agatccagca gtcatggtgc
     2941 tgccgctgga tgatcatatg gtgcatcgcg gccatggcgt gttcgacacc gctacgctga
     3001 cgcacggaat gctttatcaa ctcgatgcgc atttggatcg attgattcga tcggcccaag
     3061 gagcacgtat tccgttgccg tttcctcgag aggaactacg ccgaatcata ctcgacactg
     3121 ctgccgcaag ccgtcagcgc gaaggatcga tccgctattg gctctccgcc ggcccgggcg
     3181 gctatggcct tggtccggca gaatgcgttg gcagcagttt ttacgtgatc gtttttaagc
     3241 aggaagctta cccggaaagc tactatcgta agggaatcaa gatcatcacc agcggggtgc
     3301 cgataaaacc gccgcttttc gcgcgcatca agtcgaccaa ttatctgcct aacgtgctcg
     3361 tcctgctgga ggccaaagat catggcgccg acaatggaat tttcatcgac gagcgcggca
     3421 tggtagcgga aagctcgaat atgaatgtag cgttcgtaac cgaggatcga gtctttcgtc
     3481 atcccccttt tgaaaccatt cttccgggca tcacgatcca gagagttctc gagttcgcca
     3541 ggctgctcgt tcagcaagac gtattaagcg cgatcgatct cgccgctgtt ccggtgcaga
     3601 aaggacgcga atcggcggaa atgatgctga tcggcagctc gattaaagtc gcgccagtgg
     3661 ttgaatggga cggacaaaaa atcggcgacg gcaaacccgg acccatcgcc aagaagctgc
     3721 tggagatgtg gaacgaagac gctgcggcgg cgcaggatca gctcgtcgag gtaccgtacg
     3781 ccacaatcaa ttagtgaaac tcgcctgcgg ccgttttgct gtcggactac gattcgggtt
     3841 actcggtttt tcttgcgcgg gctgatccag ccagcttgct cccaatgtca ttgctccttg
     3901 tacgctacca atgaacaact gcacatcgat gcgcctgtgg accaatttga aacagcatct
     3961 gcttgatcgt ggagaaagag aaatctccca cccgcggagc ttaccggcag gccccatcag
     4021 gcgcgttgat attacgcccg atggggcaag aagaagtttg cgtgagccgg atatcatgag
     4081 agaaccgcct cagttattcc ctggttgtta gcctcgactg aacttcaatc tgctgttgct
     4141 gtatcggaag attggcaatc tcccgcgccg gtcccgtcag ctccagaata tgcaagccgg
     4201 tgttggcgcg gtcaacgatg tagatcagag cgcggtcgtc gacctcgaca ttgttggtct
     4261 ggatcgcaat tttgccagcg cgcggatcgg tgttggccgt cgtcgcgggg acaaagtacg
     4321 cgatctcctt cggatcaaat ggattgcgga tgtcgacggc gcgaacccct gcgttgaagt
     4381 aagcgacgaa caggatccgc ccataataga tcggcgtgaa gctctcattc gaggagtgcg
     4441 cgccgaagcg cccgccacgg cggcagaaga cgccctcggg gtccggcacg tggtagttgg
     4501 aaatgacctg aggcgtcgac tcgatggtca catcggcgaa gaaaaccatc tgatgggatt
     4561 ctccagtgca ctcgttggcc gtagactcgt tgacgattgc cagaatgtcg cgggtgccgc
     4621 ccacattgaa gggcccgagt tcaggcacgc gcacgccgag tatcgggaat gtggtgtgcg
     4681 cgcccaccag cggcgacgta tcgaactgtg cgatgacggg gaaagtaagg ttctccggcg
     4741 tcggctccgg ggggccggtc agcagcttag tccgatcgac gatctgaacc gttccgttgg
     4801 agctggtgcc gtggccgaaa taaacccggt gccccgcgct gatcgggccg tgcaaatcgg
     4861 tcggaacctc tcccgtagaa cccggctcct gcccgaccag gccgtagtca cgaatgtgaa
     4921 cagggctcct gggattactg agatcgaaca cctgggtcat gcgatcggtg cgccatccag
     4981 ggacgcccga gaccaggtag gcgatgccgg tatcacactc ccaccagttt ttatgtgttc
     5041 cctcgagtcc agacaccacg gtagtccgga gcactggcgc acttggattt ctgacatcat
     5101 aaatttgatg cgcttcattg ccatgcgc
//
LOCUS       DASUTG010000254         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_41451, whole genome shotgun sequence.
ACCESSION   DASUTG010000254 DASUTG010000000
VERSION     DASUTG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41451"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF089_05775"
     CDS             <1..238
                     /locus_tag="VF089_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX6173504.1"
                     /translation="LEENEQTVGIHVDVRHLAPTQIGQSVTVTARLLEVKGNKLRYAV
                     SATNDQNVKIGDGTHARAVINTARFAEGSEPDVT"
     gene            complement(267..1643)
                     /locus_tag="VF089_05780"
     CDS             complement(267..1643)
                     /locus_tag="VF089_05780"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6173505.1"
                     /translation="MRSWIKQLEEAGELIRIAKPVHPHTQMGALLYQSREKGLLFENL
                     DGFPGWRSLGMAPANLRHAAIAFDTTIEKLIPTAAERTLKRWPVELVSSGPVKEVIQK
                     GDQVDLTKLPAHVAGSEETPYIASGLMVARDPDSGIRNVAFHRLQIKGPRKTGALIVP
                     RHTREILNKHEARNQPMPVSIFIGHHPLYYMAAASTGAFEMDELELAGGLIGESARVV
                     KCETNDLEVPFDAEIILEGRILPNVREDEGPFSEFHDYYVAGMGKNPVIEIDCITRRQ
                     DAIFKAIQNGSEVEGCIFHKVPFGVAVYNHIKNIGGYVNLHNLLVLPGLFGIVVQMTP
                     RFYGEAKQILMGVLSSPILHPKVAIAVDEDVNIFNYWEVLWAINTRCNPQGDIIVIPG
                     TRIHPMDPTGFEPVPPGGPYWHRVGGKVMIDATKPPLCAGEQARQPFQRLKPMGWETV
                     RLEDFLPR"
     gene            complement(1767..2795)
                     /locus_tag="VF089_05785"
     CDS             complement(1767..2795)
                     /locus_tag="VF089_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6173506.1"
                     /translation="MFQASFKIPSAAAIIAAVMWATAAIAPALGQAKHEEIAIGYASP
                     SGVMMPLYVAQEQGLFKKHGLGVKELLLLRGTGPAAAQMLVAGTAPIAALGGALVEGA
                     IRGAGIAYIASTSNHLIFSIYSRSEITRPEDLKGKTVAVDAKGGSIELATIIALKQFG
                     LTLGKDVSSVYLGGPVPQLGALEKGIVDATTLSAPTTLRARKMGYKELIDIGALKLSY
                     IHTAIGVNRAFAKNNPESVEAFLKAYIEAMKMTREQPDVGIKAIARYTKIAEPEALKV
                     TYETFLPAFQQRVPYVSPDSVQGVLNFSSSAEANAQRAEDFIDHSFLKKIEASSFVKK
                     LYGNGKPQ"
     gene            complement(2913..>3126)
                     /locus_tag="VF089_05790"
     CDS             complement(2913..>3126)
                     /locus_tag="VF089_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173507.1"
                     /translation="GAPAIQLMESSDKVGVHHYGFISDDIERAAKEFAAKNARILREN
                     RDPSGKLTTIFVQDPHGLEMEIRSAR"
BASE COUNT          654 a          851 c          879 g          742 t
ORIGIN      
        1 cctggaagaa aatgaacaga cagtgggaat tcacgtcgac gtcaggcatc tagcgccgac
       61 gcagatcggc cagagcgtca ccgtcaccgc ccggcttctg gaagtcaaag gcaacaagct
      121 gcgttatgcg gtgtcggcaa ccaacgatca gaacgtcaaa atcggcgatg gtacgcacgc
      181 tcgagcggtg atcaacacag cgcgtttcgc agaaggctcg gaacctgacg taacatagcg
      241 cggattcgcg ctagatgtcg gtcgtgctat cggggcaaga agtcttccag gcgtacggtt
      301 tcccatccca tgggtttcag cctctgaaaa ggttggcgtg cctgttcgcc agcgcaaagc
      361 ggcggcttgg tagcatcgat catcactttg ccgccgaccc gatgccagta cggtcctcct
      421 ggcggcaccg gctcgaaacc cgtcggatcc attgggtgaa tgcgagttcc gggaatgacg
      481 atgatgtcgc cttgcgggtt acagcgcgta ttgatcgccc agagcacttc ccagtagtta
      541 aaaatattga cgtcttcatc gaccgctatg gccaccttcg ggtgcaggat tggactcgac
      601 aggactccca ttaagatttg cttcgcctca ccgtagaagc gcggcgtcat ttgcacgacg
      661 ataccgaaca aaccgggtag caccagaaga ttgtgcaaat tgacatagcc gccgatgttt
      721 ttgatatggt tgtagaccgc gacgccgaaa ggaactttgt gaaagatgca gccttcgact
      781 tccgagccat tctggattgc tttgaaaatt gcgtcctggc gtctggtgat gcaatcgatc
      841 tcgatcaccg ggtttttccc cataccggcg acgtaatagt catggaattc cgaaaaaggc
      901 ccttcgtctt cacgcacgtt tggtagaatg cgcccttcaa gaatgatctc tgcatcgaaa
      961 ggcacctcga ggtcgttggt ctcgcatttg accaccctgg cggattcacc gatcaggccg
     1021 ccggctaatt ccagctcatc catttcgaaa gcgccggtgc tggccgcggc catatagtaa
     1081 agcggatggt ggccgataaa aatagaaacc ggcatgggct gattgcgcgc ttcgtgcttg
     1141 ttgagaattt ctcgggtgtg ccgtggcaca atcaaagctc cggtttttct cggccctttg
     1201 atctgcaaac ggtggaaggc gacgttgcgg ataccgctgt ccggatcccg cgcaaccatc
     1261 aacccggatg cgatgtaggg agtttcttca ctccctgcta catgcgccgg taatttagtc
     1321 aggtcgactt gatcgccctt ttggatcact tctttgacgg gaccgctgga gaccagctcg
     1381 accggccatc gtttgagagt tcgttcggcg gcggtgggaa ttagtttttc gatggtggta
     1441 tcgaaggcaa tcgccgcatg gcgcaagttg gcgggggcca tgcccaagct acgccaccct
     1501 ggaaagccgt cgaggttttc gaacagcaac cctttctcac gcgactgata tagcagcgcg
     1561 cccatttgcg tgtgcggatg caccggtttg gcgatacgaa tgagctcgcc cgcttcttcc
     1621 aattgcttaa tccaactgcg catatctctc ggcattgttg tcctcgcgcc ttcgaaatct
     1681 cagagttgaa tcggcttctg cgtttcagcc gtcagcttct ctggttctgt cccatggagc
     1741 tgtcgttagc tgctgcgtaa cctggatcat tgcggcttgc cattgccata gagtttcttc
     1801 acaaaagatg atgcttcaat cttctttaag aaagaatggt cgataaaatc ttcggcgcgt
     1861 tgagcgttag cctccgctga actggagaag ttcagcacgc cctgaaccga gtcgggtgaa
     1921 acatagggca cacgttgttg aaatgcggga agaaaagttt cgtaagtgac cttgagcgcc
     1981 tcgggctcgg cgatctttgt gtacctagca atggccttta tcccgacatc cggttgttct
     2041 cgggtcattt tcatcgcttc gatataggct ttgagaaagg cctcgacgct ttctggatta
     2101 ttcttggcga aggcgcggtt taccccgata gcggtatgga tatagctgag cttgagcgcg
     2161 ccaatgtcga tcagctcttt gtagcccatc ttccgcgcgc gcaaggttgt tggtgccgac
     2221 agtgttgtgg catcgacgat gcctttttcc agagcgccta attgcggcac aggtccgccg
     2281 agataaaccg agctcacgtc tttgccgaga gtcaaaccaa actgtttcag ggcaatgatc
     2341 gtggccagtt cgatggaccc gcccttggca tcgaccgcaa cggtttttcc tttgagatct
     2401 tcgggacggg tgatttccga acgtgaataa atagagaaga tcaggtgatt ggatgtgctg
     2461 gcgatatagg caatgccggc gccgcgaatc gcgccttcca ccagagcacc gccgagggcg
     2521 gcaatcggcg cggtacccgc gaccagcatc tgcgccgccg ccggcccggt accgcggagc
     2581 aagagcaact cctttactcc gaggccatgt ttcttaaaga ggccttgttc ctgtgcgaca
     2641 tagagcggca tcatgacgcc actcggggat gcgtagccga tggcgatctc ttcgtgtttt
     2701 gcctggccga gcgcgggtgc tatggcagcg gtggcccaca tcacggccgc gatgatcgcg
     2761 gctgcgctcg ggatcttgaa gctggcttgg aacattgttc ctcctacagg ctcgtggagc
     2821 ggccttgcaa gaagctaccg acgagaagca gacgctgtaa gccgaggtaa ctttggaccc
     2881 cggcatcaaa gcaatcagat gggttcatct ggctaacgcg ccgagcggat ctccatctcc
     2941 aatccatgcg gatcctgcac aaaaatagtt gtaagtttgc ctgacggatc acgattttcg
     3001 cgcaggattc gcgcgttctt tgctgcgaat tcttttgccg cccgttcgat gtcgtcgctg
     3061 atgaaaccgt agtggtgcac accgactttg tcggatgatt ccatcaactg tatcgccggc
     3121 gcgcca
//
LOCUS       DASUTG010000255         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_337680, whole genome shotgun sequence.
ACCESSION   DASUTG010000255 DASUTG010000000
VERSION     DASUTG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337680"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1079
                     /locus_tag="VF089_05795"
     CDS             300..1079
                     /locus_tag="VF089_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6173508.1"
                     /translation="MTTDNVALNQYSSASLDQDRRGARSHNMSETERWVSLVIGGGLL
                     ALGLRQGIREGLMLAVTGGALLYRGATGQCPLYKVAGIDTSADRPRPGVSVPHGRGVK
                     IEHSVLINKSPEELYHFWRQFENLPRFMDHLASVKTEGDQRSHWVVKTIGNAEISWDA
                     EIVNDVPNELIAWRTVENADVDHAGSVRFEGSGQGTRVKVTMEYRPPAGKLGVGLAKL
                     FGQEPRQMIDSDLRRFKQLMESGEVVSAQGQSHMGSVDRSI"
     gene            complement(1407..2828)
                     /locus_tag="VF089_05800"
     CDS             complement(1407..2828)
                     /locus_tag="VF089_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6173509.1"
                     /translation="MMAIADICELNLSELSKLLATRELSSSETVKATLARLERLDGKL
                     NAFITVAGEQAIAEAKKADDEIAGGRYLGPLHGVPVTIKDLFETAGVRTTAGSKILAD
                     CVPDTDSAVVQRLRAAGAIIIGKTNLDEFGHGGTSTLSHFGPVHNPWNIEHIAGGSSG
                     GSAAAVAAGIGPLSYGTETGSSVRRPASYCGIVGYKPTFGIISRQGSFRGAWSQDHVG
                     LFARSIRDITLGLDPVAGFDARDPASVHQDTAGYTARLDANIKQLKIGVLQRFLEGVN
                     HEVSQAFETAVKVFAAAGCEVIDLDVPELSYAAMTSMMTSSAESAGINRRWFRERHQD
                     FVPHVSRGIAVGMTITASEYLTVQRARHRIREALRVAYDKVDIIASPTTARVAPLISA
                     GVKGNGDDTRHASYNHSNLLRFPSMLGLPGCSLPCGINTEGLPIGIQLIGGWFADQNV
                     LNAAFGFQSATDWHTRRPALASA"
     gene            complement(2828..3016)
                     /locus_tag="VF089_05805"
     CDS             complement(2828..3016)
                     /locus_tag="VF089_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173510.1"
                     /translation="MTPTDKVTQRLALLDGVDLSQAYVDAIAAEIEDLDRIVAELEDF
                     ARGTPWVSQQCQPAGSKA"
     gene            complement(3431..>4222)
                     /locus_tag="VF089_05810"
     CDS             complement(3431..>4222)
                     /locus_tag="VF089_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6173511.1"
                     /translation="QALSAVDIPIFAEPDQFVAQMLEELDAKKTPKAKPRSEMKEIPH
                     WNGATLSKSSPAPGQPMTLWDMAMTVREFAKGKPVTFARLPIGWPGEAYEFDGPLSFM
                     GNDGGGAVGTGPGHTIGAALALKDSDRITIGVIGDGDYLMGVNALWTASHFEIPVMIV
                     AADNRSYYNDEMHQERVAQMRDRPPQNRWIGQRIDNPRVDLVAMARAQGFDSEEPVST
                     PEALSAALKRGAEIVAGGGRYFIDSLVQPGYADAGPDQRAAVGKK"
BASE COUNT          906 a         1190 c         1194 g          932 t
ORIGIN      
        1 acggatggtt gcatctttca taagtttgcc gcctccaccg actctcctac tcctcacagt
       61 tatgcaacat ttcctctaaa cgtaaatttg tgtcctattt ttcggattgc aagtcccatt
      121 cataaacatt tcattcaagt gaggcgcacg cactcaccgc aactaaatgg atttagttta
      181 gcgcgcacgc gtttaaagaa gaaagttcca tggcatatag cttgctcaat gttgggcgat
      241 actatgagat tgagcgcagt gcccagctta tcgccaacca accatgagga gaaacatcca
      301 tgacaaccga taacgtagct ttgaaccaat attcgtcggc ttcgcttgat caggatcgcc
      361 ggggagccag atcccacaac atgagcgaaa ccgagcgttg ggtgtcgctc gtaataggtg
      421 gtgggctttt ggcattggga ctaaggcagg ggatacggga ggggctgatg cttgccgtca
      481 cgggcggtgc actactttat cgcggcgcta ccgggcagtg tccgttgtat aaagttgctg
      541 gaatcgatac ctcagccgat cgtccacgtc ctggcgtaag cgtaccgcac ggccgcgggg
      601 tgaagatcga gcattctgtt ttgatcaaca agtcgccgga ggagttgtat cacttctggc
      661 gtcagttcga gaatctgccg agatttatgg atcatctggc ttcggtaaaa accgaaggtg
      721 accagcgctc gcactgggtg gtcaagacga ttggtaatgc ggagatatcc tgggatgccg
      781 agattgttaa cgatgtcccc aatgagctaa tcgcctggcg tacagtggaa aatgccgatg
      841 tggatcacgc cggttcggta agatttgaag gaagcggaca ggggaccaga gtgaaagtga
      901 ccatggaata ccgcccgccc gcagggaaac tcggtgttgg cctggcaaag ctttttggtc
      961 aagaaccgcg gcagatgatt gacagcgatt tgcgccgctt caaacagctg atggaatcag
     1021 gtgaagtagt atccgctcaa ggacagtctc atatggggag cgtcgatcgc agcatttaga
     1081 cgctgtgccg tagccgaata gcagattttt ctgggacatt gccgctgcag tcccgtgcca
     1141 tagtcgctga tctctcctct caagcagatt agcgcgccct gagcgccacc gaatcttgtt
     1201 acagactatc ttgtgatcgt tcacttcgta gcttgcgggt gatcacgaat ctactctagc
     1261 agagtgctga aaaacctctt gcaggctgct caaaaagatc caggtgcgag gcgtgcgaaa
     1321 atcgacgagc gcaggcgtac tcccagcacg tcgcagcgag gcaacttgag cggcaacgaa
     1381 gcatatgggt ctttttcaac agcctgctag gcgctcgcga gtgcaggacg ccgcgtgtgc
     1441 cagtccgtcg cgctctggaa tccaaaggct gcgttcagaa cattctgatc agcgaaccag
     1501 ccgccgatca gttggatgcc gatggggagg ccctcagtgt taatgccgca gggaagtgag
     1561 cagcctggca gaccgagcat gctcgggaag cgcaggagat tagaatgatt gtagctggcg
     1621 tgacgcgtgt cgtccccatt gcctttgacg ccggccgaaa taagaggcgc gactctcgcc
     1681 gtggtgggcg aggcgatgat atccaccttg tcgtacgcta cccgcaacgc ttcacgaatt
     1741 cggtgccggg cgcgctgtac ggttagatat tctgaggcgg tgatcgtcat tcccactgcg
     1801 atgccccggc tcacgtgcgg cacaaaatcc tgatgccgct cgcgaaacca ccgacgattg
     1861 ataccggcgc tctcggccga gctggtcatc atcgacgtca tcgccgcata gcttaactcc
     1921 ggaacgtcta aatcgatgac ctcgcatcct gcggcggcaa agactttcac agcagtctca
     1981 aatgcctggc tcacttcatg gttgacgcct tcgagaaatc tttggagcac acctatttta
     2041 agttgcttga tgttggcgtc gagccgggct gtatatccag cggtatcttg gtgcacgctg
     2101 gccgggtcgc gtgcatcgaa cccggccacc ggatcgagac ccaaggtaat atctcttatt
     2161 gaacgcgcga acagaccgac atgatcctgg ctccaggcgc cgcgaaacga accctgccgg
     2221 ctgatgatgc cgaaggtggg cttataaccg acgatgccgc agtacgatgc cggtcgacgc
     2281 accgacgagc cggtctcggt gccgtaggag agcggcccta tgccggcggc caccgcagcc
     2341 gccgagccgc cgctggatcc gccggcaata tgctcgatgt tccacggatt atgaaccggc
     2401 ccgaaatggg acaaggtcga cgtcccgccg tggccgaact cgtctaggtt ggttttcccg
     2461 atgatgatcg ctccggctgc gcggagccgt tggactaccg ctgagtccgt atcggggaca
     2521 cagtcagcca ggattttcga tcctgcagtt gtgcgcacgc ccgccgtctc aaacaagtct
     2581 ttaatcgtta ccggtacgcc atgaagagga cctagatagc gcccgccggc gatttcgtca
     2641 tcggcttttt ttgcttcggc aatggcctgc tcgccagcaa cggtgataaa ggcattgagc
     2701 tttccgtcga ggcgctccag ccgagccaga gttgccttga ccgtctccga tgatgacagt
     2761 tcgcgagtcg caaggagctt ggaaagttcg gacaggttca attcgcaaat atcggcgatg
     2821 gccatcatca agccttactg ccggcaggtt ggcattgctg cgagacccac ggagtgcctc
     2881 gagcgaaatc ttccagttcg gcgacgattc tgtcgagatc ctcgatctcg gcggcgattg
     2941 catcgacgta agcctgagaa agatccacgc cgtcgagaag cgccagacgc tgggtgactt
     3001 tatctgtggg tgtcattttg tactcatatc agctcgcgga acatattgcg gcagagcatc
     3061 cactgactat cgacacgaag tgtgtcatga gcttatatcc gcccacagag aggatgaaag
     3121 cgcgactatc ctgcatcttc tcagaactcc tttccccttg atggggaaag gccgggatag
     3181 gggtgctcgg aactgatcaa accggatcac ccctaccttt gtccttcccc ctcaagggga
     3241 aggatcagac gttgtcgccg tcggtcacct ggtatccggt cggctactca gcggctcaaa
     3301 gccaaacttt cgaacgaggt cgcgaaaagc acgaagtaaa ctcccgaagt aattgcttat
     3361 ccttcgtgaa cttcgtgcat ttcgtggcga acttttcggt tctcttctgc aggcggcctg
     3421 cacgaatcgg ctacttcttc ccgacagccg cccgctgatc cggtccggca tcggcatagc
     3481 ccggctgtac caacgaatcg atgaaatagc gcccgccgcc ggcgacgatt tcagcgccgc
     3541 gcttgagcgc tgctgacaga gcctctggcg tcgacaccgg ctcctcgcta tcgaagccct
     3601 gtgcccgcgc catcgccaca agatccactc gtgggttgtc gatgcgctgg ccgatccagc
     3661 gattctgcgg cggccggtcg cgcatttgcg cgacccgctc ctgatgcatc tcatcgttgt
     3721 agtatgaccg gttatcggcg gccacaatca ttaccggaat ctcgaaatga gaagcggtcc
     3781 atagcgcgtt gactcccatg agataatcgc catcgccaat taccccgata gtaatgcgat
     3841 cactatcctt gagcgccaag gcggcgccga tagtatgccc cgggccagtg ccgacggcgc
     3901 cgccaccgtc attgcccata aatgacaacg gtccgtcaaa ctcgtaggct tcgccaggcc
     3961 agccgatcgg cagacgggca aaggtcacgg gttttccttt ggcgaattcg cgcacagtca
     4021 tcgccatgtc ccataacgtc atcggctgtc ccggcgccgg tgacgacttt gataaggttg
     4081 caccgttcca gtgcgggatc tctttcatct ccgatcgcgg tttggctttg ggagttttct
     4141 tcgcatcgag ttcttcgagc atctgcgcga cgaactgatc cggctcggcg aagattggaa
     4201 tatcgacagc ggacagggct tg
//
LOCUS       DASUTG010000256         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1078881, whole genome shotgun sequence.
ACCESSION   DASUTG010000256 DASUTG010000000
VERSION     DASUTG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078881"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF089_05815"
     CDS             <1..673
                     /locus_tag="VF089_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6173512.1"
                     /translation="STLIGYSEILLNRELPAETRDILKTMLSNGQDLSNMMDGLLDFS
                     RMEADAVPNSFEHINVKDLLGGLQLMTQRLIRGRPIQFRINIEDAIETINSDPRKLQQ
                     ILLQLLTNALKFTEKGAVEVSIATIDDQSGAFLEIAVSDTGIGIDKNDQEIIFEGFRQ
                     LDGSSKRRFGGTGVGLSLCSKLAAALGGKISVKSEVGLGSVFSVFLPLTVAAGHSEPS
                     QAAQA"
     gene            738..1388
                     /locus_tag="VF089_05820"
     CDS             738..1388
                     /locus_tag="VF089_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6173513.1"
                     /translation="MMTGKAFVSELLVMVNDFCRTLDAQPPAYSYIPLTSDEERVRVM
                     KSRLYNEVRAAELFGTWLKTTPELEVKAIMAESAHEEMEHAQLLSKRIGDFGFEPFDY
                     QPLPAQMAMFNAIEGLTDTCQRMAGFSLAGEAVATYLTQKSLKASTVPDWIKQPYRRI
                     TQDEEGHGSIPQEILQRYAVTGERQEAARRAVAMRLVLFREYLNSLDRWAMGKDRW"
     gene            complement(1393..1734)
                     /locus_tag="VF089_05825"
     CDS             complement(1393..1734)
                     /locus_tag="VF089_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6173514.1"
                     /translation="MLDYNRGRNIAINELENEAKKQAIIDNLRPICLCNKIRKGIVVK
                     AIQAGAKTFEMVSKRTGIGTGPCGAKRCGAAVRGMLGEPVTTCRDCGWAILLGPLPLV
                     CPRCESVQNEK"
     gene            complement(1728..2741)
                     /locus_tag="VF089_05830"
     CDS             complement(1728..2741)
                     /locus_tag="VF089_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX6173515.1"
                     /translation="MDYSDLMKLASGHVEARIVQAAVELEIFEAIGTHALEAASVANS
                     LDLEPRATELMLNALTALALLRKREDRFSLTDLSAKYLKRDAPGYLGGMIRFEASLWH
                     CWTSLTEAIRSGHPVRAADMYQDDPKETTIFIDAMDSLVKARGDAEIMAEAFDWDTAH
                     ELLDVGSGPATYPIALCRRFPNLRATIFDLPATIALTDCYVREAGLTSRIRLISGDYR
                     VDDIPGSYDVVFLSNIIHSESFEVNRRLMVNLSSVLKTGGSVIVKDHILDSTRINPPV
                     GALFSLLMLLTTQAGRCYAFNEVEAWMSAAGLTHIRQIELPAPLNSSLIMATKENHVE
                     NSC"
     gene            complement(2751..4145)
                     /locus_tag="VF089_05835"
     CDS             complement(2751..4145)
                     /locus_tag="VF089_05835"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6173516.1"
                     /translation="MDFLSAYEHRRGGRQKKKEEELGREVARILSGMANADGGTLLVG
                     VEADKTITGLPYDSNELIALLQAPENLLRPALTPSSEAIRVGNLQLLKFEVGSSVEIY
                     RIAGGRSYYRLAAETPALPSEQIHSLKEAKKTVFYERQQALNASWQDLDSDLVREFLH
                     KINDAREPETVLAQSYHLLDNSRALPAPNMAALLLFAKDPNRWHPRCGIDFVKYEGSE
                     RQYGAALNVVKRLRFDGPLVRLIDEAVGRIKEHIRERTLLHDLFFRERLEYPTFAWQE
                     AVVNAVAHRDYALTGASIEVWMFDDHITVRSPGLPPLPVSLEQIRQQKSIHFSRNPLI
                     VRVLADMGYLREMGEGVPRMFQEMETYGLNPPEFSANGFIFTVTLRNAPVYDDDTLRW
                     LNQFAASSVNQRQRRLLAYAYCHGKTFSTTEYERIAEVDRDTAYRDIRLMVKSGIVAP
                     VKPKSRSYRIIERL"
     gene            complement(4254..>5411)
                     /locus_tag="VF089_05840"
     CDS             complement(4254..>5411)
                     /locus_tag="VF089_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6173517.1"
                     /translation="GKPLINPERLKNLLIELIKIDSLSRKEYDVAMRLKREMEDLGAQ
                     VSIDDAGERVGGNVGNLIAHFTGTAPKAMPILLSAHMDTVVPGEGIVPILEGDILRTD
                     GRTVLGGDDKSGVAIICEVLRVIKENRFPCSDVDVVFTICEEAGLIGAKCLDVSRLRA
                     RTGLVLDSDSVGFLFTKAPAANRMEFQIHGLEAHAGVCPERGVNAIKVAADGIAQMSL
                     GRIDHETTANIGVIEGGMAVNIVPNSVILRGEARSHSQEKLDRQTEHMLSCLQDAAAR
                     HTLEVAGAHFSASVDAKIERDYDRMDVSDGAPIVQLVRAAAKNLRVEVKTLATGGGCD
                     ANVLNQKGLEVANLSTGMREIHTVKEWLDLKDLNLSAQMVLEVVRLNAANP"
BASE COUNT         1256 a         1441 c         1445 g         1269 t
ORIGIN      
        1 cagcaccctg ataggatata gcgagatcct cctcaaccgc gaactaccgg cggaaacacg
       61 cgatattctg aagacaatgt tgagtaatgg acaggatctg tcgaatatga tggatggcct
      121 actggacttt tcgcgaatgg aggccgatgc ggtgcccaac agtttcgagc acatcaatgt
      181 aaaagatctc ctgggtggtc tacagcttat gacacagaga cttatccgcg gccgtcccat
      241 tcagttcaga atcaacatcg aagacgccat tgagaccatc aacagcgatc cgagaaaact
      301 tcagcagata ctgcttcagc tgttaaccaa tgcgcttaag ttcactgaaa aaggagcggt
      361 tgaagttagc atagcgacta tcgacgatca aagtggcgca tttctggaga tcgctgtctc
      421 ggatactgga atcggtatag ataagaacga tcaggagatc atcttcgagg ggtttcgcca
      481 gctggacggc tcatcgaagc gccgtttcgg cggcactgga gtgggcttga gtctgtgcag
      541 caagcttgct gcagcgctcg gtgggaagat cagtgttaag agcgaggtgg gtttgggaag
      601 cgtcttcagt gtgtttttgc ccctcacggt ggccgccgga cattcggaac caagtcaggc
      661 ggctcaagcg tgataatgtg atcggccgag gttcgcatgt ttgactcatt cgatgtcctt
      721 agttacgtag taaatctatg atgacaggga aagctttcgt cagcgaattg ctggtgatgg
      781 taaatgattt ctgccgaact ctggatgcgc aaccgccagc ctatagctac attccgctta
      841 ccagtgatga agaacgcgtt cgggtgatga agagccggct gtataacgaa gtgcgcgcgg
      901 ccgaattatt cggaacatgg ctcaagacaa ctcccgagct ggaagtcaaa gccataatgg
      961 ccgaatcagc gcatgaggaa atggagcacg cgcaactcct atcaaaacga atcggtgatt
     1021 tcggctttga accgtttgat tatcaaccgc tgcctgctca gatggcgatg tttaatgcga
     1081 tcgagggact cacggacacc tgtcaacgga tggcgggatt ttcgctagcc ggagaagccg
     1141 tggcaactta tttgacccag aagagtttga aagcttcgac agtgcccgac tggatcaaac
     1201 aaccgtaccg aagaattacc caggatgaag aagggcacgg cagcatcccc caagagattc
     1261 tgcagcgtta cgcggtcact ggagagcgtc aggaagcagc gcgcagggca gtggccatgc
     1321 gcctcgtgtt atttcgtgag tacttgaaca gcctggaccg ttgggcaatg ggcaaagatc
     1381 gttggtaact cgttatttct cgttttgaac ggattcgcag cgtgggcaca cgagcggcaa
     1441 cggtccgagt aagattgccc agccacagtc cctacaggtc gttaccggtt ctcccagcat
     1501 tccacgaacc gcggccccgc aacgcttcgc cccgcaggga cccgttccga tgcccgtgcg
     1561 tttactaacc atctcaaaag tctttgcgcc ggcttggatg gccttaacca ctataccttt
     1621 gcgtatttta ttgcaaagac aaatcgggcg gagattatct atgatagcct gtttcttggc
     1681 ctcgttttca agttcgttaa tagcgatatt ccttccccga ttatagtcta acacgaattc
     1741 tcaacgtggt tctccttagt ggccattatt agagacgagt tcagcggcgc cggcaactct
     1801 atctgtctga tgtgcgtcag accggctgcc gacatccagg cttccacttc gttgaatgca
     1861 tagcaacgcc cggcttgggt ggttaggagc atgagcaaac taaagagggc gccgaccggc
     1921 gggttgatgc gcgtgctatc tagaatatga tccttgacga taacgctgcc gccggtttta
     1981 agcacagagc taagattgac catgagccgt cggttcactt cgaagctttc gctgtgaatg
     2041 atgttggaaa gaaaaaccac atcatagctg ccgggaatgt catcaacccg gtaatcaccg
     2101 ctaatcagtc ggattcggct ggttaaaccg gcttctcgga cgtaacagtc ggtgagtgcg
     2161 atagttgccg gcaagtcgaa aatggtagcg cggagattcg gaaatcttcg acaaagcgcg
     2221 atgggatagg tggctggtcc cgaacccaca tcgagcagct cgtgagcggt gtcccaatcg
     2281 aaggcttcgg ccatgatctc cgcatcgccc cgcgccttga caagagaatc catcgcatcg
     2341 atgaaaatag tggtttcctt gggatcatcc tggtacatgt cggcggcgcg aacagggtgg
     2401 ccactccgaa tagcctccgt caatgaggtc caacaatgcc agagggaggc ttcgaaacga
     2461 atcatgccgc cgagatagcc gggagcgtct cgcttgagat actttgcgga taaatccgtg
     2521 agagaaaatc ggtcctcccg cttccgcaac aacgctagag cggttagggc gtttagcatc
     2581 aactcggtag cgcgaggttc tagatcaagc gagttggcaa cacttgcggc ttccaaggca
     2641 tgggttccta tggcttcgaa aatctccagc tccaccgccg cctgaactat tcgggcttcg
     2701 acgtggccgc tggccaattt catcagatcg gaataatcca caccgatttc tcacaagcgt
     2761 tcgatgattc gatagcttcg gcttttcggt ttcacaggcg ccacgatacc gcttttgacc
     2821 atcaggcgga tatctcgata ggcagtgtcc cgatcgactt ccgctattct ctcatattcc
     2881 gttgttgaga aagttttgcc atgacagtac gcgtaggcca gcagccgacg ctgtcgctgg
     2941 ttgaccgaag aggcggcgaa ttgatttaac cagcgtaggg tgtcgtcgtc gtagaccggc
     3001 gcatttctaa gtgtgacggt gaagataaat ccattggcgg aaaattccgg cggattaagc
     3061 ccataggttt ccatctcctg aaacattctc ggaactcctt cacccatttc acggagataa
     3121 cccatatccg ccaggacgcg cacgatgagc ggattgcggg agaagtgaat gctcttttgc
     3181 tggcgtattt gttccagact cacaggaagg ggcggaagtc cggggcttcg cacagttatg
     3241 tgatcgtcaa acatccaaac ttcgatggaa gctccggtta aggcgtagtc ccggtgagcg
     3301 acggcgttaa caaccgcttc ctgccatgcg aaggtcggat attccagacg ttcgcgaaag
     3361 aaaagatcat gcaatagggt ccgttcgcgg atatgctcct tgatccttcc aaccgcttca
     3421 tcgatcagac gaaccaacgg gccgtcaaac cttaatcgct taacgacatt caaggccgcg
     3481 ccatactgcc gctcggagcc ttcatatttg acgaaatcga tgccacatct tggatgccag
     3541 cgatttggat ctttagcgaa cagaagcagc gccgccatat ttggggcagg aagcgcgcga
     3601 gaattgtcaa gcagatgata agactgagcg aggacggtct cgggttcccg cgcgtcattg
     3661 attttatgca aaaactcccg gacaaggtcg gaatcgagat cctgccaact cgcattgagc
     3721 gcctgctgtc gctcatagaa cacggtcttc ttggcttctt tcagcgaatg aatctgttcc
     3781 gatggcaacg caggggtctc cgccgccaat cggtagtagg atcgcccgcc agcaatacgg
     3841 taaatttcga cactggaacc gacctcgaat tttaggagct gtagattgcc cactcgaatc
     3901 gcttcagatg acggcgtcag agctggtcga agtagatttt ccggcgcctg cagcaatgcg
     3961 atgagctcat tagaatcgta ggggagtccg gttatcgttt tgtccgcttc gacgcccacc
     4021 agcagtgttc cgccgtccgc gtttgccatg ccggagagaa tgcgcgcaac ctctcgaccg
     4081 agctcttctt cctttttctt ttggcgcccg ccgcgccgat gttcataggc gctcagaaaa
     4141 tccaaaaact gtccgcgttc ctgtttaagc aggaatccaa ttgcatcgcc gtgcatagct
     4201 gaagcgctag agcaggtatc gttcgataaa ttccgcgttc tgggatccct tttttacggg
     4261 ttggcggcgt taagtcgaac cacctccaac accatctgag ctgacagatt aagatcctta
     4321 agatcgagcc attcctttac ggtgtggatc tcgcgcatac cggtagatag atttgcgacc
     4381 tccaacccct tttggttgag tacattggcg tcgcaccctc caccggtggc gagagttttg
     4441 acttctaccc gcaaattctt ggccgcggca cgcacgagtt ggacgatggg agctccgtcc
     4501 gaaacatcca tgcggtcata gtcgcgctcg attttggcgt ccacactggc gctaaaatgt
     4561 gctccagcta cttccaatgt gtgacgcgcc gcagcgtctt ggaggcagct tagcatgtgt
     4621 tctgtctgtc ggtccaattt ctcctggcta tgactgcgcg cctcgcccct cagaatcact
     4681 gagttaggca cgatgttgac cgccatgcct ccctcaatga cgccgatatt cgcggtggtt
     4741 tcatgatcaa tacgtcccaa ggacatctgc gcaatgccgt cggcagcaac tttgatcgcg
     4801 ttgacgcctc tctccggaca gacgccagca tgtgcttcaa gtccgtgaat ttggaattcc
     4861 atgcgattgg cggcaggggc tttagtaaac agaaaaccta ccgaatcgct atccagcact
     4921 agtccggtac gagcgcgaag ccggcttacg tccaaacatt tggcgccgat gagcccggct
     4981 tcctcacaga tcgtgaaaac cacatcgacg tcgctgcagg gaaagcgatt ttctttgatt
     5041 acgcgtagca cctcacagat gatcgcaacg ccgctcttgt catcgccgcc aagaaccgtg
     5101 cgcccatcgg ttcgcagaat gtcgccctcc aggatcggga cgataccttc tcccggcacg
     5161 accgtgtcca tgtgagccga gagtaagatc ggcattgctt ttggcgctgt gccggtgaag
     5221 tgcgcaatga gattgccgac gtttccgccg actctctcgc cggcatcgtc aatcgagacc
     5281 tgtgccccca aatcttccat ctcgcgctta aggcgcatcg cgacatcgta ttccttacgc
     5341 gaaaggctgt cgattttaat caactcaatc aggagattct taagtcgttc gggattaatc
     5401 aatggtttac c
//
LOCUS       DASUTG010000257         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1967861, whole genome shotgun sequence.
ACCESSION   DASUTG010000257 DASUTG010000000
VERSION     DASUTG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967861"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..169
                     /locus_tag="VF089_05845"
     CDS             5..169
                     /locus_tag="VF089_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX6173518.1"
                     /translation="MAQAIPIVKVTHPLAKVTHEAAIGSIDQKQLETLMAHGLTPEQA
                     VEVVVAGLLR"
     gene            212..1324
                     /locus_tag="VF089_05850"
     CDS             212..1324
                     /locus_tag="VF089_05850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX6173519.1"
                     /translation="MNEPVTLFLCGDVMTARGIDQALPYPGDPRLYEPCVENASEYIR
                     LAEQANGPLPRPVDFSYIWGDTLEVFQREKPRAKIINLETAVTRSDTHWQSKDVHYKM
                     SPDNIPSLTAAGVDCCAVANNHILDWGIPGLIETLDVLDKVKIKHAGAGLNLQEARAP
                     AIVKTGEGTRVIVFSLGSTSSGIPAEWSARENLPGLHLIESYPEDLVRFLAKEISAIR
                     QTGDIVIVSTHWGSNWGYKIPRSENRLAHRLVEEAGADIVHGHSSHHVKAIEVYRERL
                     ILYGCGDFFNDYEGISGHEQFRGDLGLMYFADINSSTGKLIALRLIPTQIRRFRLNRA
                     SATDSQWLADLLNREGKRFNTAVQQSDENIFKLRWH"
BASE COUNT          567 a          613 c          607 g          533 t
ORIGIN      
        1 ggccgtggcc caagccatcc ccattgttaa ggtcactcat cccctggcca aggtcacgca
       61 tgaagcggcg atcggcagca tcgatcaaaa gcagctcgag acgttgatgg cccatggcct
      121 gacgcccgag caagcagtcg aagtcgtggt ggctgggctg cttcgttgaa tcgctttggt
      181 gcgcagtccg tgctgaaaag gcgctagccg catgaacgaa ccagtaactt tgtttttatg
      241 cggtgacgtg atgaccgcgc gcgggatcga tcaagcccta ccctatccag gagatccacg
      301 gctctacgag ccatgcgtcg agaatgcgtc cgaatatata aggcttgccg agcaggcaaa
      361 cggtccgctg ccgcgcccgg tcgatttcag ctacatctgg ggcgacacgt tggaggtgtt
      421 tcagcgcgaa aaaccacgag cgaagataat taatttagaa acggcggtca ctcgtagcga
      481 tacacactgg cagagcaaag acgtccacta caagatgagc ccggacaata tcccgtctct
      541 tacggcagcc ggagtcgatt gctgcgccgt tgcgaacaat catatcctcg actggggaat
      601 tcccggactg atcgaaactc tcgacgtgct cgataaagta aagatcaaac atgccggcgc
      661 cggtcttaac ctccaggaag cccgggcgcc ggcaatcgtg aaaactggag aaggcacaag
      721 ggtaatcgtc ttcagcctgg gctcgacatc aagcggcatc ccggccgaat ggtcggcccg
      781 ggaaaacctg cctggcctgc atctgataga atcctacccg gaagatcttg tgcgcttcct
      841 agcgaaagaa atcagcgcga ttagacaaac aggtgacatc gttattgtct cgacgcactg
      901 gggaagcaac tggggttata aaattccccg cagcgagaat agactcgctc atcggctcgt
      961 cgaggaagct ggagctgata ttgttcacgg ccattcgtcg caccatgtca aagcaatcga
     1021 agtctacaga gagcgcctga ttctttacgg ctgcggcgat tttttcaatg attatgaggg
     1081 catcagcggc cacgagcaat ttcgcggcga tctcggtctg atgtactttg cggatatcaa
     1141 ttccagtacc ggcaaactta tcgcgttgcg gctgatacca acccaaatcc gccgctttcg
     1201 attgaaccgc gcgtccgcaa ccgactcaca gtggctggcg gatctcctca atcgagaagg
     1261 gaagcgattt aatactgcag tgcagcagag cgatgagaat attttcaagc tccgttggca
     1321 ttaaagatga agttccaggt gcatcaacct gcctgagaag ccggaggttg ttttcggaat
     1381 gacgcgcaca gcggcttatg gtcacatctt aaagtgaacg cggtttccgc tttttcgatc
     1441 ggagggcata tatacaagga tgtgctcaag agacgacgag agcgccgcaa ttcgatcgaa
     1501 tgaaacacgc gcttaatatt tcaatgttga ttatcatctg cagtgcggta cttagctgtg
     1561 gaccgacatc aatcctgttg cttaacgtga agacaaacga tctccgtcac tgtccggtgg
     1621 gcgaagacag cgatgcctgt atcaaacgac tggaagagct cggctatatc agagcggacc
     1681 gtttgacccc cgcgcagaaa gcaagcctcg gattatccct tccatactag atgggcatcg
     1741 tgctcatctg cccagcagga gctaggcgcc tccgtgttct tgaaagagtg gaggatgtcg
     1801 agatctcctt gaagcttctt ttcacatcct tgacaccagt tcgtctcttt tctgtcggtg
     1861 tcacgtcgcg aattgaatgc attcagcggg gctggttgtt ccatggcgat tgtcgctcga
     1921 cagcgccaca agtcagcagc gtcagtcgta aagaaggtag tcgtaacgat gatactcgcc
     1981 catctttctt tcccccgtaa gagcttggca tgtgatcgtt aaccttgtca caaatgatag
     2041 gtcaagggct tttcttccga tctttgcgaa agcgtatttc ggaggaaatg tatcaacggt
     2101 gatttggcaa tcttccttcc tcaatccatc gaccgaaatt cctatgctat ccaaggaact
     2161 catactcgct caaacgcaga aaccataccg ctgaggcaca cttcaaaagt ccagcttgcc
     2221 tgccaacgtg atcgttctcg ctagctactg ccagcggatc gtctaatgac agcagattag
     2281 ctcgaaacct ctggcgtatg tattgcgttg taattcatta
//
LOCUS       DASUTG010000258        13180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_337838, whole genome shotgun sequence.
ACCESSION   DASUTG010000258 DASUTG010000000
VERSION     DASUTG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337838"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VF089_05855"
     CDS             complement(<1..935)
                     /locus_tag="VF089_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6173520.1"
                     /translation="MVEIKSLIDPEGGLIDRRIFADREIYQLERERIFARCWLYLGHE
                     CEIPNPGDFVTAYMGEEPVILWRDLNRQIHAYLNVCRHRGNRVCRADRGNAKVFTCSY
                     HGWTYSSEGKLAIVPMPDAFPGLDREQWGLIPVAQLSAYKGLIFATFDPSAPPLLDYL
                     GDMAWYLDILLDRRQGGTEVCGPHRWIVDANWKTAAENFGGDGYHISYTHGSARELGV
                     DTTSNSTRKWNKGCQIACGNGHILVSWLTPPDDAGPWFAQPDEELLNYMKEHAGEIEQ
                     RLGRVRAREISPSAGTVFPNFSVHWLTRTIRVWQPR"
     gene            1222..1728
                     /locus_tag="VF089_05860"
     CDS             1222..1728
                     /locus_tag="VF089_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6173521.1"
                     /translation="MTAEAEYAAVPITVTVNEVKHEAVVEPRTLLVYFLREQLGLTGT
                     HVGCDTSQCGACTIHLNDRAVKSCTILAVQANGKKITTIEGLAHNDEFHPVQEGFREK
                     HGLQCGYCTPGMIMTAADLLRTNANPSEEEIKHALEGNLCRCTGYVNIVESVKWAAEK
                     IGGKNVSR"
     gene            1715..2584
                     /locus_tag="VF089_05865"
     CDS             1715..2584
                     /locus_tag="VF089_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6173522.1"
                     /translation="MFPANFGYVAPRSLDEALQLLKRHGENAKLLAGGHSLLPAMKLR
                     LASPETLIDLGTVPGLSGVRREGDRLVIGALTVHADVGSSDLVRKYFPGLIDAASVIG
                     DVQVRNCGTIGGSVAHADPAADFPVILTALNASFVLKSTDSNRTVAADEFFTDYFTTA
                     MEPGEILTEILVPLPAAGSGTAYQKLAHPASGYVVVSAGALITRQQSGSCASARIAIG
                     GLGSGPIRAIASETELQGKVLNPQNIAAAAAKAAEEADPDDDIYATAEYKRHMATVLA
                     RRAIEAAAQRSTT"
     gene            2695..5004
                     /locus_tag="VF089_05870"
     CDS             2695..5004
                     /locus_tag="VF089_05870"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6173523.1"
                     /translation="MTTEHNSDGLIGASIRRVEDPLLISGKGCYVDDIKLPGMLHMMV
                     LRSAYPHAKIISINTDAARSMKGVVAVVTGPDLPENLNINAQVMFPGQKIPPHPVLAR
                     GAVHAPGTPVAAVVAESRAIAQDACNAIEVEYQALPSIQNAEDALKPGAPLAREELDS
                     NICYTAVKKGGDVDKAFAQADHIVRMHIVSPRQVALAIEPRGVAANPDPMGKSLTVWL
                     STQGPHRARADLATTLGIPENKIRLIAPDVGGGFGSKGPLYREDVLTSYMALKLRRPV
                     KWIATRSDDFVTTIQGRDQAMASELALKKDGTMLGLKVRVVANLGGYLQSSTAGPPQR
                     MMGMAPGCYQIRDCHVEVVGVFTNTVPTGPYRGAGRPESVLNIERLIDKAAKDLGIDR
                     LEIRRKNFIRPEQFPYKTAVGVEYDSGDYFKSLDEAIKIANYQDLMRQRDERRARGEI
                     VGVGVSTFVEPSGGAGFESGTVRIERTGEITVLTGSSSHGQGHETVWAQIAAEMLKTD
                     MEHVTVLHGDTYVSQQGTGTFGSRSAVVGGGALATAAQRVIDKAKRIAAHLVEASAED
                     MVQADGGFSVAGVPEKKVTWRQIAAAAYGGKIPPGMESGLQETCFFDPRREAWGYGAH
                     VCVISINRETGEPKIEKLVLVDDCGVLINPMIVEGQIHGGVAQGLGEALREQMVYSED
                     GQVLTGTLMNYAVMRATDMPRLILGETVTPNPFHPLGVKGVGEAGTNGAPPAVANAVM
                     DALAPFGIDHIDMPYTAPKLWRAIRQSGG"
     gene            5328..6407
                     /locus_tag="VF089_05875"
     CDS             5328..6407
                     /locus_tag="VF089_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173524.1"
                     /translation="MAPRQSKFLFMACLLISVASLHLFLFTSEVAAQASFFQGKTITI
                     IQGRGPGGTGDLRVRALVPFLQKYIPGNPTIVMEYMPGGGSRKAANHLFNSARPDGLT
                     IGNLSAGMVSLAVLGESGILYDLDKFNYLGSAYSTYHSVFISRKEAGLDSLDKLRAAK
                     EIRVGGQSVGFSTYNEARLFCYVIGCREPRFVTGFSGPELDPALMRGEIDARSTGPDS
                     LLQRNKEWLDQGLIHIHAIMETPKDEKHPHPLFKKLPVLEDLAKSDKERKVIALQRAF
                     RVAGSPFVLPPATPKDRVKLLQEGFRKTFEDPEFFKEYDKLTGDRPTPLLPENHEKAI
                     AEIPRDRETIEIFKKLVGAGPLPPR"
     gene            6449..7504
                     /locus_tag="VF089_05880"
     CDS             6449..7504
                     /locus_tag="VF089_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173525.1"
                     /translation="MKNVILGGVLGLCGMMLGLLPSFSYSQSDFYAGKTVTLVATTAP
                     GGTGDLRVKAMLPFLKKHIPGNPTVVIQYMDGGGGRKGANHVYSSVAPDGLTIGAASG
                     AIIGLAVMGEKGVSYDIDKFIYLGTPESENHYVIYTRKELALDSQEKLASRPGIRIGA
                     QTVGHVSYVAGRLFAYFLNFKEPNFIAGYTSREADVALVNGELDARANNATSVLRRNP
                     EWLEKGLMNFHSIMEVPLGDKHPRLGHLPEIGTFARSETEKKLISLWRAFRGVGSPYI
                     LPPGTPKDKVAILEEAMRKTFKDPEFAPYFKKIVADDASPLGPVELRKVIAEIPRDAE
                     TIDMLRKFAGPGPLPPR"
     gene            7547..9085
                     /locus_tag="VF089_05885"
     CDS             7547..9085
                     /locus_tag="VF089_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6173526.1"
                     /translation="MESSLVDAALTGFLNILHFNVLFALLIGVSIGTFTAVAPQGLGM
                     PLVYAIMLPVVIKWKPITGIALLIGASSVSAICAAYLPVLFGIPGGSGSQATILDGYP
                     MGRRGEARRALGASFMAGGMGCLIGTFTLALAIPVARPLIYLMGSPELFVIVLWGLSM
                     VAVLAGRRPLKGLLAACFGLLLATVGQQAQSGIMRFVFDEPYLLDGLSISIIALGLFG
                     IPAAMDLAVKRLGVEQQPAPLTGKLLDGVKDTLREWWLVVRCSFIGVWVGIVPGIGSS
                     VVDWLAYGHAAQSSKGGGQSFGQGDVRGVIAPESANDAKDGGDLVTTLLLGFPQGVST
                     ALFIVALLAWGYLPGPELIKKNPEIIYSIVWIQGISGIVGTLVGFGLASQLAKLAQVR
                     YSIMVPTIFVFVLMGAFSVTRDPADLLTVVVFGVFGYFMRRFGYPRPALILGLVLGSL
                     LETYLYRSMASYGFTWLTRPAVIVLLILAATSFFFAIRGRMINAAKLPAAALRDISKD
                     QDDS"
     gene            9075..9548
                     /locus_tag="VF089_05890"
     CDS             9075..9548
                     /locus_tag="VF089_05890"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6173527.1"
                     /translation="MTANFTLFMFAVFAGATYWGWSWPYIAKLMPVYVVAIPGLILAT
                     LQLYRDGTGWEKRRENAAEGLEMDDVHNIKLDRATELRRTLVFFAWFIGGAVGIWLLG
                     IVYSLPLLVFLYALIDGGENFRASLIMGICTYLLIWGLFEYMLETRWPPGLLFGY"
     gene            9694..10428
                     /locus_tag="VF089_05895"
     CDS             9694..10428
                     /locus_tag="VF089_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6173528.1"
                     /translation="MQIIEKEFTTKEHGIAGFLSHPERRTPGPALLLIHPKGGMTDYI
                     KIESRKYARLGYSTFAINVFEQLGYPAATHIETGSQIQAKTADPEFTRVLTEGWRFLL
                     SQPHVNRQRVAVCGYCMGGRIAIHFVAATPEVRAFVGYYPTVRDEPTSALRPVHPSEA
                     VSKIRCPSIILYGADDVTTVLPVQEKMWQAFKNNGQPLEWHFFPFGGHGYVDPGAVGY
                     HAHAAELSWPLVVDFMERHLQWGDAA"
     gene            10502..10921
                     /locus_tag="VF089_05900"
     CDS             10502..10921
                     /locus_tag="VF089_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173529.1"
                     /translation="MDKITKPTVVDTEKQAQWGRPTGRNYVRPTQTDRAHIALNCYEP
                     GANDEMHCHPGSDHTFLVIEGECTMKGLNEGEEYKLTQHQAVHVPAGYFYQLNNTGSG
                     RLILYQVSTEPNPKPKIGKVLYGIDTSLKEHLLEPVG"
     gene            10935..11189
                     /locus_tag="VF089_05905"
     CDS             10935..11189
                     /locus_tag="VF089_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173530.1"
                     /translation="MTFEQVLQWCRNNGADARGIYRARDFWIRRSDDHLPDNLPPMGA
                     LFHWDVQLSETELAVSPSDMERLVNGKMTLEQFKATHRRG"
     gene            11441..12721
                     /locus_tag="VF089_05910"
     CDS             11441..12721
                     /locus_tag="VF089_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6173531.1"
                     /translation="MRLTLEIYPITAIRFGHSTRLDGTSLNVDVEQLRALLLEDQTLT
                     AVDLEIASPGESCRAGPVFDIIEPRAKAPDSSPDFPGVLGPPHTAGFGTTHVLEGAAV
                     TVLREKSPGDSRGATGYVLEMSGEAAAGSKYGSLQHLIILPKTQSDLPDHARHKAYRL
                     AGLKTAVYLARAAFDQAPASRITFDTLDTVPGERDGLPRVAYVGQIFSRQRKPELDER
                     ILYGVGTDGMLPVPLHPDEWLDGALLPSYHTSLGGAETYFYQNHPVITELYRRHRARE
                     LSFAGTVATIASADNFDRERNCRFAANLVRTVLKAEAAILTKFGGGVPHTDLSETARL
                     LEATGIKTAVQVTDLARDSRVESALLFNFPEVNAIVCIGGNSTSWKVPRVERVIAASA
                     ELKEVLGGPLHLESLNVVGVANQQGGSRLRAMVY"
     gene            12958..>13180
                     /locus_tag="VF089_05915"
     CDS             12958..>13180
                     /locus_tag="VF089_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HEX6173532.1"
                     /translation="MRIAHYLNQFFAGVGAEEKAGLPLETREGAVGPGKLFEQLIGGD
                     ARVVLTLVCGDNYAIENQDQMVAAAVDKIR"
BASE COUNT         2823 a         3559 c         3867 g         2931 t
ORIGIN      
        1 cgcggctgcc agacacgaat tgtacgggta agccagtgga cagaaaaatt cgggaaaacg
       61 gtaccggccg aaggcgaaat ctcccgcgcg cggaccctgc cgagccgctg ctcgatctcg
      121 ccggcgtgtt ctttcatgta gttgagtaac tcttcgtccg gttgtgcgaa ccacggcccg
      181 gcatcatcgg gcggggtcag ccaggaaaca agaatatgac cgttaccgca ggcaatctgg
      241 cagcctttgt tccacttgcg agtggagttc gaagtggtat cgactccgag ttcgcgcgcc
      301 gatccgtgag tgtaggagat gtgataaccg tcgccaccga aattttccgc ggcggttttc
      361 cagttggcat cgacaatcca gcgatgcggt ccacagactt cggtgccgcc ctggcggcga
      421 tccaacaaga tatccaggta ccacgccata tcgcctaagt agtcgagcaa tggcggcgcc
      481 gacggatcga acgtcgcgaa gattaaccct ttgtaggcgc ttagttgagc gacaggaatg
      541 agaccccact gctcgcggtc cagtccggga aaggcgtccg gcattggcac aatcgcgagt
      601 ttgccttcac tgctgtaggt ccagccgtga taggagcagg taaaaacttt ggcgttgccg
      661 cggtcggcac ggcacacgcg attaccgcga tggcggcaaa cattcagata agcgtggatc
      721 tgccggttca aatcgcgcca caggatcacc ggctcttcgc ccatatatgc ggtaacaaaa
      781 tcgccggggt tgggaatctc gcattcatgg ccgagataga gccagcagcg cgcaaagatc
      841 cgttcgcgct cgagttggta aatctctcga tcggcaaaga tgcgccggtc gatgagaccg
      901 ccttcgggat cgattaggga cttaatttcg accatgctgc ctcctggcct tccgacagct
      961 ttcgccttgg tgatagtagg accgtgaagc actgtcaagg taatgctccg acttcgtggt
     1021 gaggctgtca agcttccata atatggtacg gctagaattt gcctgccaag atcgggtctc
     1081 tgagcttgaa cttttgaacc gttgaacggt tgaacgtgga acttaatatt ccaagaactt
     1141 gagacccaga ccaatcgggt gtaagacacc agattatccc caaaccggat gcactgaact
     1201 acgattggag gaagatcgag catgactgct gaggccgagt acgcggctgt cccgataacc
     1261 gtaaccgtca acgaagtgaa gcatgaggcc gtggtcgagc cacgaacttt gctggtttat
     1321 tttctacgcg aacaactggg gttgaccgga acgcatgtgg gttgcgatac cagccagtgc
     1381 ggcgcctgca cgatccacct taacgatcga gcggtaaagt cctgcacgat tttggcggtc
     1441 caggccaatg gcaaaaagat tactactatc gagggactgg cgcacaacga tgaatttcat
     1501 ccggtgcagg agggttttcg cgaaaagcat ggactgcagt gcggttactg cacgcccggc
     1561 atgatcatga ccgccgctga tttgctccgc accaacgcca atccaagcga ggaagagatc
     1621 aagcacgctc tggaaggcaa tctgtgccgc tgtaccggtt acgtcaatat cgtggaatcg
     1681 gttaaatggg ccgcggagaa gatcggaggg aaaaatgttt cccgctaatt ttggctacgt
     1741 cgccccacgc tcgctagacg aggcgctgca attgttaaag cgccacggtg aaaacgccaa
     1801 gttgctggcc ggcggccaca gtttgctgcc cgcgatgaag ctgcggctcg cctcgccgga
     1861 gaccttgatc gatttgggca ctgtgcccgg tctcagcggt gtgcgtcgag aaggggatag
     1921 gctggtgatc ggcgctttga cggtgcacgc ggatgtcggc tcatccgatc tcgtacgcaa
     1981 atattttccg ggattgattg acgcagcctc ggtgattggc gacgtgcagg ttcgcaactg
     2041 tggcactatc ggcggcagtg tcgctcatgc cgatccggcg gccgacttcc cggtgattct
     2101 aacggctctc aatgcgtcgt ttgtactgaa gtcaactgat agcaaccgga ctgtggcggc
     2161 agacgaattc tttaccgatt atttcactac ggcaatggag cccggcgaga tactgacgga
     2221 aatcctggtg ccgttgccag cggcgggaag cggcaccgcg tatcagaagc tggcgcatcc
     2281 ggcttcggga tacgtggtcg tcagcgccgg cgcgctgatt acgcgccagc aatccggctc
     2341 gtgcgcatcg gcacgcatcg ccatcggcgg tttaggcagc ggccccattc gagcgatcgc
     2401 cagcgagacg gaattgcaag gcaaggtcct caatccacaa aacatcgccg ccgccgcggc
     2461 taaggcggcc gaagaggcgg accccgatga cgacatttac gccactgccg aatacaagcg
     2521 gcacatggca acggtgctgg cgcgccgggc gatagaagcc gcggcacaac gcagtactac
     2581 ttaaaagccg tgctcgtgag cgtgatcgtg gtcgtaagcc aaactcggga attcaacgtg
     2641 tcgcccgctt tcacacgaac acgactccgg taactcttag acgaggttca acgcatgacg
     2701 actgaacaca acagcgatgg tttgatcggc gcttcgatcc gacgggtaga agatcccctg
     2761 ctgattagcg gcaaagggtg ttacgtcgac gacatcaaat tgccgggcat gttgcacatg
     2821 atggtgctgc gcagtgccta tccccacgcc aagattattt cgattaacac cgacgcggcg
     2881 agatccatga aaggggtcgt tgccgtggtg accggaccgg acctgccgga aaatttgaac
     2941 atcaacgctc aggtgatgtt tccgggtcag aagattccac cgcatccggt gctggcgcgc
     3001 ggcgccgtgc acgctccagg gacaccggtc gctgcagtgg tcgcggagag ccgggcgatt
     3061 gcccaggacg cgtgcaatgc catcgaggtg gaataccaag cgctgccgtc gatacagaac
     3121 gccgaggatg cgctcaagcc gggcgcgccg ctggctcgtg aagagttgga cagcaacatc
     3181 tgttatacgg cagtaaaaaa gggcggcgat gtcgataaag cctttgccca agcagatcac
     3241 attgtccgta tgcacattgt cagcccgcgc caggttgccc tggccatcga accgcgcggc
     3301 gtcgccgcca atcctgatcc gatgggcaag agcctgacgg tctggctttc cacccaaggc
     3361 ccgcaccggg cgcgcgctga tctcgccact accctaggca ttccggaaaa caaaatccgc
     3421 ttgatcgcgc ccgatgtcgg cggcggattc ggtagtaaag ggccgcttta tcgcgaagac
     3481 gtcttgacta gttacatggc tctgaagttg cgccgcccgg tcaaatggat cgcgacccgc
     3541 agcgatgact ttgtcactac gatccagggc cgcgaccagg cgatggcttc cgagttggcg
     3601 ctcaagaaag acggcacgat gttggggctg aaggttcgcg ttgtcgccaa tctcggcggc
     3661 tatctgcaat cgagcacggc cgggccgccg cagcgcatga tgggcatggc gccgggctgt
     3721 tatcagatta gagactgtca tgtcgaagtc gtgggtgtgt ttaccaatac ggttccgacc
     3781 gggccgtatc gtggcgccgg ccggcccgag tcggtgttga acatcgagcg gctgatcgac
     3841 aaggcggcca aggatctcgg catcgatcgg ctggaaatcc gccggaaaaa ctttattcga
     3901 ccggaacagt ttccctacaa aacggcagtc ggcgtggagt acgattcggg cgattatttc
     3961 aaatcgctgg acgaggcaat caaaattgcc aattaccaag atctgatgcg ccagcgtgat
     4021 gagcggcgcg cgcgcggcga aatcgtcggt gtcggcgttt ccacttttgt cgagccgagc
     4081 ggcggcgccg ggttcgaaag cggcaccgtg cgtatcgagc gtaccggcga gatcaccgtt
     4141 cttaccggat cgagctccca tggccaggga catgagacgg tttgggctca aatcgccgcg
     4201 gagatgttga agaccgatat ggagcacgtc accgtgctgc atggcgatac ttacgtttcg
     4261 cagcagggca cgggaacctt cggcagccgc agcgcggtgg tcggcggcgg cgctttagcg
     4321 accgcggcgc agcgtgtaat tgacaaggcc aagcgcatcg ccgcccatct agttgaggca
     4381 tctgccgaag atatggttca ggctgacggc ggattttccg tggccggcgt tcccgaaaag
     4441 aaagtcacgt ggcgccagat cgccgcggcg gcgtatggcg ggaaaattcc gcccggcatg
     4501 gaaagtggtc tccaggaaac ctgctttttt gatcccaggc gtgaggcgtg gggctatggc
     4561 gcccatgttt gtgtaatcag catcaatcgg gaaaccggcg agcccaagat cgagaaactc
     4621 gtgcttgtcg atgattgcgg cgtgctcatc aatccgatga tcgttgaagg gcaaattcac
     4681 ggcggcgtgg cgcaaggtct cggtgaagca ctgcgcgaac aaatggtcta cagcgaagat
     4741 ggccaggttc tcaccggtac gttgatgaac tacgcggtga tgcgcgccac cgatatgccg
     4801 cggctgatcc ttggtgaaac tgtcacgccc aatccgtttc acccgttagg cgtcaaaggc
     4861 gttggtgaag cgggtaccaa cggcgcgcca ccggcggtgg ccaatgcggt gatggatgcg
     4921 ctggcgccat ttggcatcga tcacattgac atgccctaca ctgcgccaaa gctctggagg
     4981 gcgattcgtc aatcgggcgg ctagctaatg aggtgtcaga agacgctttc gtgcttgcgt
     5041 ccgtgttcgc cggatcctct gccggcgatg ttcgggcttt ggcttttcta caagtgattc
     5101 tccgacgggg taaaagcccc tcggtcaatg caccgctcgc gatctctcgt cttcgctgcc
     5161 ggtcagattg ctgccgctca gccaagattc aacccacgat cgaccctcgt gccgacctcg
     5221 gtaaagttga ttctttttga aactaggcgt tgacaaagaa ccggaaccgt aattgaatcg
     5281 caaccgtttt gcagataccg gcttttctct aacctgagga gatctatatg gcgccaagac
     5341 aatccaagtt tctatttatg gcatgccttc tgatcagtgt ggcatcgctg catttatttt
     5401 tgttcacctc agaggtcgcg gcacaggctt cgttttttca aggtaagacg attacgatta
     5461 ttcaaggccg cggtcccggc ggcacaggcg atctccgggt gcgggctttg gtgccgtttc
     5521 tgcagaaata catcccagga aatcccacca tcgtcatgga gtacatgccc ggcggcggca
     5581 gccgcaaggc ggccaatcat ctctttaact ccgcccggcc cgacgggctc acgatcggca
     5641 acttgagcgc tgggatggtt tcgttggcgg tgctcggcga atccggaatc ctgtacgacc
     5701 tcgataagtt caattatctc ggttcggcct acagcacgta tcactcagtg tttataagca
     5761 ggaaagaagc gggtctcgac tcgctggata aacttcgagc tgccaaggag atcagggtcg
     5821 gcggccaatc cgtcggcttc tcgacttaca acgaagcgcg gctcttctgt tatgtcatcg
     5881 gttgccggga gccgcgattc gtaaccgggt tttccggtcc ggaactcgat ccagcattaa
     5941 tgcgcggtga aatcgatgct cgttcgaccg ggccggatag cctgctacag cgcaacaaag
     6001 aatggcttga ccaaggattg atccatatcc acgcgatcat ggaaacgccc aaagatgaga
     6061 agcatcccca tccactattc aaaaaacttc cagtccttga ggatttggcg aagtccgata
     6121 aggagcgaaa ggtcattgcg ttgcagcgtg cgttccgagt cgcaggttca ccattcgtgc
     6181 tgccaccagc gacgcccaag gatcgcgtca agttgctgca ggaaggattt cgcaagactt
     6241 tcgaggaccc ggagtttttc aaggaatacg ataaacttac cggcgatcga ccgaccccgc
     6301 ttttgccgga aaatcatgaa aaggcaattg cagaaatccc acgcgatcgg gaaaccatcg
     6361 agatttttaa aaagcttgta ggagcaggcc cgttgccacc gcgctaatta atcgacattg
     6421 agagacttgg cttgaaaagg agagatcaat gaagaacgtt atcctcggtg gcgtgttagg
     6481 gctttgtgga atgatgttgg ggctattgcc ttcgttttca tactctcaga gtgattttta
     6541 tgccggcaag accgtcaccc tggtcgcgac cacggctccg ggaggaaccg gagatctgcg
     6601 ggtgaaagcc atgctgcctt tcttaaagaa acatattccc gggaacccaa cggtggtgat
     6661 ccagtacatg gacggcggcg gtggtcgcaa gggagccaat cacgtttata gctcggtcgc
     6721 acccgatggc ctcaccatcg gtgccgccag tggagcaatc attgggcttg ccgtgatggg
     6781 cgaaaagggg gtctcttacg atatcgataa atttatttac ctcggcacgc ccgagagcga
     6841 aaaccactat gttatctata cgcggaagga acttgccctg gatagtcagg aaaaactcgc
     6901 ttctcgccct ggaattagaa ttggtgcgca gacggttgga cacgtgtctt atgtggcggg
     6961 aaggcttttc gcttattttc tgaacttcaa ggaaccaaat tttatcgctg gttacacttc
     7021 gcgagaggcg gatgtcgcct tagtcaacgg cgagctcgat gctcgagcca ataacgcgac
     7081 ctcggtgctg cgccggaatc cggagtggct cgagaaaggc ctgatgaact tccactcgat
     7141 catggaggtt ccgttgggtg ataaacaccc gcggctaggt catttgccgg aaatcggcac
     7201 cttcgccagg agcgaaacgg aaaagaaact gatctcgctg tggcgtgctt ttcgaggggt
     7261 tggctcaccg tacatcttgc ctccgggcac acccaaggat aaggttgcga tccttgagga
     7321 agccatgcgc aaaaccttca aggatcctga gtttgctcca tatttcaaga agatcgtcgc
     7381 cgatgacgca tccccgctgg ggccggtgga attgagaaaa gtgatcgccg aaattccgcg
     7441 tgacgcagag accatagata tgcttagaaa gtttgcggga ccaggtccgc taccgccgcg
     7501 gtagaaactc ctccattagc caagagaaca cgcgagaaga cttgatatgg aaagttccct
     7561 ggtagacgcg gccctcacag gatttttgaa tatacttcat ttcaacgtgc tctttgctct
     7621 tctgatcggc gtcagcatcg gtacctttac cgcagtggcg ccgcaaggct tgggcatgcc
     7681 gcttgtctac gcgatcatgc tgccggtcgt gatcaaatgg aaaccgataa ccggtattgc
     7741 cctgctcatc ggtgcaagct cggtgagcgc catttgtgcg gcctacctgc ctgtactttt
     7801 cggcataccc ggaggctcgg gttctcaggc aacgatactc gacggttatc cgatgggaag
     7861 gcgcggcgag gcacggcgcg cattgggagc ctcgttcatg gccggcggca tgggctgcct
     7921 tatcggcacg ttcactctcg ccctggcgat tcccgtggcc agacctctta tctatttgat
     7981 gggatcgccg gagcttttcg tcatagtcct ttgggggctc agcatggtgg cggtgctggc
     8041 ggggcggcgg ccacttaagg gactgctggc cgcgtgcttc ggcttgctct tggccacggt
     8101 cgggcagcaa gcgcaaagcg gcatcatgcg tttcgttttc gacgaacctt atctcttgga
     8161 tggtctctct atcagtatta ttgctctggg cctcttcggc atccccgcag cgatggatct
     8221 agcggttaag agactcggcg tagagcagca gccggcccca ttgaccggaa agctcctcga
     8281 tggggtgaag gataccttgc gcgagtggtg gctggtggtg cgctgcagct ttatcggcgt
     8341 atgggtagga atcgttcctg gaatcggttc ttcggtggtg gactggctcg cctatggaca
     8401 tgcggctcag agcagtaaag gcggcggcca gtctttcgga cagggcgacg ttcgcggtgt
     8461 catcgctccc gagagcgcga acgacgccaa ggacggcggt gatctagtta cgactttatt
     8521 gctcggcttt cctcaaggag tttcgacggc gctatttatt gtggcgctgt tggcttgggg
     8581 gtatctgccc ggtcccgaac tgatcaaaaa aaatccggag attatttaca gcatagtctg
     8641 gattcagggt atttctggaa tcgttgggac tctggtcggc ttcggtcttg ccagccagct
     8701 cgcgaaactg gcccaggtgc gttattcgat catggtcccg acgatttttg tttttgttct
     8761 gatgggagcc ttcagcgtga ctcgcgatcc cgccgatctt ctcacggtcg tcgttttcgg
     8821 agttttcggc tactttatgc ggcgctttgg ttatcctcgt ccggccctga ttctgggact
     8881 cgttttaggc agcttactgg agacctatct ctatcgttcc atggcaagct acggctttac
     8941 ctggctcacc cggcctgcag tgattgtgct gctgatcttg gcggccacgt cattcttctt
     9001 cgcgatacgt ggccggatga taaacgccgc caagctaccg gcagccgctc tcagagatat
     9061 ctcaaaggat caagatgaca gctaacttta cattgttcat gttcgccgtc tttgccggcg
     9121 ccacttattg gggttggagt tggccttaca tcgccaagct catgcccgtt tatgttgtgg
     9181 ctataccggg cctgattctc gccacgttgc aactttatcg cgatggcacc ggttgggaaa
     9241 agcgtcgaga gaatgccgct gaagggctgg aaatggacga cgtgcacaac atcaagctgg
     9301 acagggccac tgagctccgg cggactctgg tgttttttgc ctggtttatc ggcggcgcgg
     9361 tgggtatttg gcttttgggt atcgtctaca gcctaccgct cctggtgttt ctttatgcct
     9421 taattgatgg gggagaaaat tttcgtgcat ctctgattat gggcatttgc acctatctgc
     9481 tgatctgggg tcttttcgaa tacatgctgg aaacgcgctg gccaccggga ttgttgttcg
     9541 gctattgatc gcatgcggtt gcgaacgtta cgtccgtaga tacctttcgt tctgagcgcg
     9601 cccatcaatc taccatcctg caaacccctt tacgcacgtc tgcggatcat gttacgtatc
     9661 ggagaatagc ttacaggcga taaggataac agaatgcaga taatcgaaaa ggagtttacc
     9721 actaaggagc acggcatcgc cggtttcctg tctcatcccg aacgcaggac gccagggccc
     9781 gccttacttt tgattcatcc caaaggtggc atgaccgact acatcaaaat agaatcacgc
     9841 aagtacgcca ggctgggtta cagcactttt gcaattaacg ttttcgagca actcggctat
     9901 cccgcggcaa ctcacatcga aaccggatca cagattcaag ccaagaccgc cgatcccgaa
     9961 tttacccggg tgctcaccga aggctggcgt tttttgctat cgcagccgca cgtgaatagg
    10021 caacgagtag ccgtgtgcgg ttattgtatg ggcgggcgca ttgcaattca tttcgtcgcc
    10081 gcaacccccg aggtgcgcgc gttcgtcggt tattatccga cggtgcggga cgaaccgacg
    10141 tcggccttac ggccggttca tccttccgag gctgtgagta agattcgttg cccgtcaatc
    10201 attttgtacg gcgctgacga tgtcaccacg gtattgccgg tgcaggaaaa aatgtggcag
    10261 gcgtttaaaa acaacggtca gcccttggag tggcacttct ttccattcgg cgggcacggt
    10321 tacgtcgacc cgggcgctgt cggctatcac gcccatgccg ctgagttgtc gtggccgctg
    10381 gtggtggatt tcatggagcg ccatcttcag tggggcgatg cagcgtaacg tcatttcgca
    10441 aacttcgcgc aaaggtcaaa ccagttgaac aagtctattc gtaaactcat ggaggtttcc
    10501 aatggataaa ataaccaagc cgaccgtcgt tgatactgag aagcaggcgc aatggggccg
    10561 gcccacggga agaaattatg tccggccgac acagacggat cgcgcccaca tcgcgctcaa
    10621 ctgctatgag ccgggagcga acgatgagat gcattgccat ccgggatcgg accatacttt
    10681 cttggtgatc gaaggcgagt gcaccatgaa aggtctcaat gagggcgaag aatataagtt
    10741 aacccagcac caggcggtgc atgtgccggc cggttatttt taccagctca acaacaccgg
    10801 ttccggacgg ctgattcttt atcaggtgag caccgagccc aatcctaagc ccaagatcgg
    10861 caaggtgctg tatggcattg ataccagctt gaaagagcac ctgctcgaac ctgttggcta
    10921 atctggagga agagatgacc ttcgaacagg tgttgcagtg gtgtcgtaac aatggggccg
    10981 atgccagagg aatttatcga gcaagggatt tctggatccg ccgctccgac gatcatctgc
    11041 ccgataattt gccgccgatg ggcgcgcttt ttcactggga tgtgcagcta agcgaaacag
    11101 agttggcggt cagcccgtct gacatggagc gtctggtgaa cggtaaaatg actctcgaac
    11161 aattcaaagc cactcaccgt cggggttgat tgtctaagat cagccgatga ggcatcggtc
    11221 gatcggtaac acaaaggccg cagcaagatt ttgctgcggc ttttgcttga acgccggata
    11281 gtgcggcgcc agattggctc gaatggagcg ccctggatga tggctgaaag ccaagccaac
    11341 ctatcgtcag atatcgatca cgctatcagt ctaagtcatc tcgttgacaa ctccgcgggc
    11401 cgcacaatag agtctagatc agtagaagga ttaagctagt atgcggctga cactggaaat
    11461 ttatccgatc actgcgattc gcttcggtca ttcgacccgc ttggacggaa cctcgctgaa
    11521 tgtcgatgtt gagcagctgc gagcattgct ccttgaagac caaacgttga ccgcggtgga
    11581 tttagagatc gcttctcctg gagaaagctg ccgggccggt cccgtttttg acatcatcga
    11641 accgcgcgcc aaggcgccgg attcgagtcc tgattttccc ggcgttctgg gaccgccgca
    11701 caccgcgggg ttcggcacaa cccatgtgct cgaaggggcg gcggtgactg tgctccgaga
    11761 gaagtcaccc ggtgattctc gcggcgccac cggttatgtc ctggaaatga gcggcgaagc
    11821 ggcggccgga tcgaagtacg gctcgctgca gcatcttata atcctgccca agactcaatc
    11881 ggatctgccc gatcacgcgc gccacaaagc ttatcgacta gccgggctta aaaccgccgt
    11941 gtatctcgcg cgcgcggcat tcgatcaggc gcctgcctca cgcatcacat ttgacaccct
    12001 cgataccgtt cctggagaac gtgacggcct gccccgggtc gcctatgtcg ggcagatttt
    12061 ttcacgtcag cgcaaaccgg agttggacga gcggattctt tacggtgttg gtaccgatgg
    12121 catgctgccg gtgccgctgc atccggatga atggctcgac ggtgcgttgc tgccgtctta
    12181 tcacacctct ctcggcggcg cggagactta tttttaccag aatcatccgg tgataaccga
    12241 gctttaccgg cgccatcgtg cgcgcgagct cagtttcgcc ggcaccgtgg caacgattgc
    12301 atcggccgac aatttcgatc gcgagcgtaa ctgccgcttc gctgccaacc ttgtccgtac
    12361 ggttctgaaa gccgaggcgg cgatacttac aaagttcggc ggcggcgtgc ctcacaccga
    12421 tctttcggaa actgcgcgct tgttggaagc aacgggcatc aaaaccgccg tgcaggtcac
    12481 cgatttggcg cgagacagca gagtggagtc cgctctgctg tttaactttc cggaggtgaa
    12541 cgccatcgtc tgcatcggcg gcaacagcac cagctggaag gtgccgcgcg tggagcgagt
    12601 catcgccgca tctgcggagt tgaaggaagt gctcggcggg ccgttgcatc tggaatcgct
    12661 taatgtggtt ggcgttgcca atcagcaggg aggatcaagg ctccgcgcca tggtttacta
    12721 acaggctggt gaaaaagcct catatgcttc gttgcgcttg cttgatttgc ttcaacgtac
    12781 tgaagcgtat gcctcagctc atcgaacgtc gcacgcctag cctctaagtc tttttgacca
    12841 gcctgtgaga gtttcttcgg aactgctcag gcagaatagg cccttcggag aaaagcgctt
    12901 tgaacttttg aacggcttga acgatttgaa cttatttgcg agatagggag actgacgatg
    12961 cgaatcgcac attacctgaa ccaattcttc gccggagttg gcgctgaaga gaaagcgggc
    13021 ctgccgctcg aaacccgtga aggtgccgtt ggccctggaa aactattcga gcagctgatc
    13081 ggcggcgacg caagagtcgt gctgaccttg gtgtgcggcg ataattatgc gatcgagaac
    13141 caggatcaga tggtcgcagc cgctgttgac aagattcgtc
//
LOCUS       DASUTG010000259         4177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1376460, whole genome shotgun sequence.
ACCESSION   DASUTG010000259 DASUTG010000000
VERSION     DASUTG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376460"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF089_05920"
     CDS             complement(<1..753)
                     /locus_tag="VF089_05920"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX6173533.1"
                     /translation="MTQISRLFLTSVTAVLLLFCGWLLIRAGAGQSEAASPKYVFLFL
                     ADGGGIAHLEITRQYNRQVLNEGMAISDKIMREGSVGLMTTHAANSLSTDSAAAATAL
                     ASGCKAQIGALGVCADGSVPDTAMEHARQRGMRIGLVTNSTIYDASPAAFVCHVSNRR
                     HIASIIDRYLELEPDVILGGGKEHFLPKSQLGSRRNDETDAIAAFQKKGYQLVSNKLE
                     LERTSGGKLLGLFSDKEMSFEIEREKNSEPSVY"
     gene            complement(755..1471)
                     /locus_tag="VF089_05925"
     CDS             complement(755..1471)
                     /locus_tag="VF089_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173534.1"
                     /translation="MPARLIVAWLVHLYSALGAVIAFIAILFVEQLQFREAFFLLTLA
                     VAIDASDGTLARAARVKELLPNFDGDRLEDIIDYFNYVVVPCLLLIRANLLPPQDALW
                     LIAFPLVASAYGFCQKQAKTADHFFLGFPSYWNVVALYLYVLRAPLWLNAFLILALSI
                     LVFVPIRYIYPSRSPYLRSWTNTLGILWGIAIVAMIYWLPEPPREIIFASLIFPIYYV
                     CLSLWLELRRVRLKKIESPG"
     gene            complement(1481..2089)
                     /locus_tag="VF089_05930"
     CDS             complement(1481..2089)
                     /locus_tag="VF089_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6173535.1"
                     /translation="MMIKLYTFPPSTNSRKVRIALVEKGLEFERVNVDLTKREQKNPE
                     YLKLHPFGQIPTLDDEGFIIYDSTIINEYLEDEYPYPPLMPKDSEGRARARLMEDFRD
                     NHFNAYFVHIIQEIRKPEGERDSQRIDNAKAEIAKAFDRIERELEGKEYLAGAFSLAD
                     IAFMPNIDLLDRFGIAIDPKYKNTIAWIDRLKARPSFAASAT"
     gene            complement(2310..4163)
                     /locus_tag="VF089_05935"
     CDS             complement(2310..4163)
                     /locus_tag="VF089_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6173536.1"
                     /translation="MRLSNRCDSTRARLVGLLVCLDLLFTFSAWPLEISSSTSSLDVL
                     KNHARFLASEKLTGRGIDTPGIRFARDYIAAEFAKLGLQPGGDNGSYLQSFEVATGVR
                     VKRPTSLTLGNQPALTLDQEWTPLGFSVSKKVEAPVVFAGYGITLKEYGYDDYEGIDA
                     KGKIVIVLRYEPPPKHANSPFRRYPNHSVHAALRSKAKNARDHGALGMILVDLRHQGD
                     AKGELISTTNSLWRAGPSLVAAQVRHAPIEKWLNEHGVSLTELRDKIDHAETPASIEV
                     PAGKATLEVNLEAIRQPTENVVAVLPGSDPRLKKESVVIGAHYDHIGFGHYGTRKSST
                     EGQVHHGADDNASGTAVLLDVARRLSRTDIKPARTIVFAAFSAEELGLFGSRHYVNHP
                     AVPVSSTKAMINLDMVGRLRDGRVTVFGTRSAQEFSGIVKSAATELGLEISESDGIGR
                     SDNMSFYRKKIPALHFFTGIHSDYHGPGDTWDKLNLEGMAKIAELVLATVGSIANTNA
                     PLNFVSLPSGSGAQAISEDGASTAYLGSIPDYSGGNEGVRLVGVSAGSPAALAGLREG
                     DVIVMLAETKVQNLEDLMLALGRRKPGDEVEIVVLRGTQPLKLKTVLRARG"
BASE COUNT          991 a         1124 c         1085 g          977 t
ORIGIN      
        1 gtagaccgac ggctcgctgt ttttttcgcg ttcgatttcg aagctcattt ctttatcgct
       61 gaacagcccg agaagtttgc cacctgaagt ccgctcaagc tcgagtttgt tgctaaccag
      121 ctgatagcct ttcttttgaa acgcggcgat tgcatcggtt tcgtcgttgc gccggcttcc
      181 gagctgactc tttgggagga aatgctcttt gccgccgccc agaatgacgt caggctcgag
      241 ttccagataa cggtcgatga tcgaggcgat gtggcggcgg ttagaaacat gacagacaaa
      301 tgcagcaggg ctggcgtcgt agatggttga attcgtcaca agtccgatgc gcatgccgcg
      361 ttgtctggca tgctccatcg cggtatcggg cacagaaccg tcggcgcaaa cacctaaggc
      421 gccaatttgg gctttgcaac cgctggccag cgccgtggcc gcggcggcgg agtctgtaga
      481 aagagaattc gccgcatgcg ttgtcatcag tccgacgctt ccttctctca ttattttatc
      541 gctgatcgcc atgccctcat taagaacttg ccgattgtat tgacgggtaa tctcgaggtg
      601 agcgatgccg ccaccgtctg caaggaacaa aaaaacgtac ttgggcgaag ccgcctcgct
      661 ctggcctgcg cctgctctta tcaagagcca cccgcaaaat aagagcaaga ccgcggtgac
      721 cgaggtgagg aagagacgag aaatttgcgt catcctaacc tggactttcg atttttttga
      781 gtctgactcg gcgcaattca agccataagg agaggcagac atagtaaatc ggaaagatca
      841 gagatgcgaa gattatctct cgcggtggtt caggcaacca gtaaatcata gccacaatcg
      901 caatgcccca gagaatgccc agagtgtttg tccatgaacg caagtacggg ctccgacttg
      961 gatagatata tctaattggc acgaacacaa ggatcgatag cgccagaatc aaaaacgcgt
     1021 tgagccagag cggtgctctt agaacgtaaa ggtataaagc cacaacattc cagtacgagg
     1081 gaaagcccag aaaaaagtga tccgccgttt ttgcctgctt ttggcaaaac ccatacgcgc
     1141 ttgcaaccag tggaaaagca attaaccaga gcgcatcctg aggcggcaat aaattggccc
     1201 ggataagcaa gagacacggc acgacgacat aattgaagta atcaatgatg tcttccaggc
     1261 ggtctccatc gaagttcggc agcaactctt tgacccgcgc ggctctggcc agagtgccgt
     1321 cggacgcgtc aattgcaacc gccaatgtca gtaagaaaaa cgcttctcga aattgtagct
     1381 gttcaacaaa aagaatcgct ataaaggcga tcactgcccc gagggcgctg tagagatgga
     1441 ctagccaagc tacgatcagg cgtgcgggca tccagccgga ttatgtcgct gaagcggcaa
     1501 agctgggccg cgcttttaga cgatcgatcc aagcaatcgt gtttttatac ttcggatcga
     1561 tagcgatacc aaaacgatcg agcaggtcga tgttcggcat aaacgcaata tcggcgagac
     1621 tgaaagcgcc ggccaaatac tctttgcctt ctaattccct ttcgattcgg tcgaacgctt
     1681 tcgcaatttc tgccttcgca ttgtctattc gctggctgtc cctttcgcct tccggtttgc
     1741 ggatttcctg gatgatgtga acaaagtacg cgttgaagtg attatcgcga aaatcttcca
     1801 tcaagcgcgc acgggcgcgt ccttccgagt ctttgggcat caacggcggg tagggatact
     1861 cgtcctctag atattcgttg atgatcgttg agtcgtaaat aataaacccc tcgtcgtcca
     1921 aagttggaat ttgaccgaac ggatgaagct tgaggtactc cggattcttc tgctcccgct
     1981 tggtaagatc aacattgacc cgctcgaatt ccaacccttt ctcgactagg gcgattctaa
     2041 cttttcggga attcgtcgat gggggaaaag tatacaactt gatcatcatc agcctcctag
     2101 aagtgcacca cagcgttgac taaaaattat actaacataa ggtattacac gctgcgagca
     2161 tttttcctac gactggaggc gaaaacattg tatctaccgg cagactcgcg acatgctcgc
     2221 tctcagcggg agaaacatga tcgcagagca ttcttgatct agccgttggt tcacggccaa
     2281 cagtttacgc ccgcaactca ttggacgggc taaccccgag cccggagcac tgttttgagc
     2341 ttgagcggtt gcgtccctct aagcaccacg atctcgacct catcaccagg tttccttctg
     2401 cccaaggcga gcatcaaatc ctccaggttc tgaaccttgg tttcagccaa catgacgatc
     2461 acgtctccct cacgaagccc ggccagcgca gccgggcttc ccgccgaaac tccgacgagc
     2521 cgcacgcctt catttcctcc cgaatagtcc gggatgctgc cgagataggc cgtggaagcc
     2581 ccgtcttcgc tgatcgcctg tgcccccgaa cctgatggca ggctgacaaa gttcaatggt
     2641 gcgttggtgt tggcgattga accaaccgta gccaggacca gctcggcaat cttcgccatg
     2701 ccttccagat tgagcttgtc ccaagtatcg ccgggcccat gataatcgga atgtatgccg
     2761 gtaaagaagt gaagcgccgg aatctttttg cggtaaaagg acatgttatc gctccgtccg
     2821 attccatccg attcgctgat ctctaggccc aattccgtgg ccgcactctt gacgataccg
     2881 ctgaattcct gtgcggaacg tgtgccgaat acggtcactc ggccatctcg taaacggccc
     2941 accatatcga gattgatcat cgccttggtc gaagatactg ggacggcggg atgattgacg
     3001 tagtgtcgcg agccgaaaag tcctaactcc tcggccgaga aggcagcaaa aacgattgtt
     3061 cttgcgggtt tgatatcggt tcggctcagt cgtcgtgcga catctagcag taccgcagtg
     3121 ccggaggcat tgtcatccgc gccatgatga acctgtccct ctgtgctcga cttgcgcgta
     3181 ccatagtggc cgaaaccgat gtgatcgtaa tgcgccccga tcaccacgct ctcttttttg
     3241 agacgaggat ctgaaccggg aagaacggca acaacgttct ccgtgggctg gcgaatcgct
     3301 tccagattca cctccaaggt cgccttgccg gccggcacct caatcgacgc cggtgtttcc
     3361 gcatggtcga ttttatcgcg caactccgtt agcgaaacac catgctcgtt caaccatttc
     3421 tcgattgggg cgtgccttac ctgcgcggct accaaactgg gtccggcacg ccacaagcta
     3481 tttgtggtgg agatgagttc tcccttggca tcaccttgat gacgcagatc caccaagatc
     3541 atgccgagag cgccgtggtc ccgtgcattc tttgctttgc tacggagggc agcatgaacc
     3601 gaatggttcg gatacctacg gaacggactg ttggcgtgct tgggcggcgg ttcgtagcgc
     3661 agaacaatga ctatttttcc tttagcatcg atgccctcat agtcatcata gccgtactct
     3721 tttagcgtaa tgccatagcc ggcgaaaacg actggcgctt ctactttttt tgaaacggaa
     3781 aaacctaatg gagtccactc ctggtccaat gtcaatgctg gttgattgcc taacgtgaga
     3841 cttgtcggcc gcttgaccct cacgccggtg gcaacttcaa acgattgcag gtagctgccg
     3901 ttgtcacctc caggttgcag gccgagcttg gcaaactcgg ctgcgatgta gtcacgggcg
     3961 aatcttatgc cgggagtatc gatcccgcga ccggtgagct tttctgatgc gaggaaacgc
     4021 gcatgattct ttagaacgtc aagactagat gttgaagatg agatctcgag aggccaagca
     4081 gaaaacgtaa acagtaagtc gagacaaaca agcagcccaa ctaggcgagc gcgcgtcgag
     4141 tcacaacgat ttgagagtct cataagttaa ctttttc
//
LOCUS       DASUTG010000260         7420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1821675, whole genome shotgun sequence.
ACCESSION   DASUTG010000260 DASUTG010000000
VERSION     DASUTG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821675"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..540
                     /locus_tag="VF089_05940"
     CDS             403..540
                     /locus_tag="VF089_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173537.1"
                     /translation="MHPFVSGVPFRTKIIDEFIRYAKGFPKVWFARRIEIANWCLEKG
                     Y"
     gene            1360..1587
                     /locus_tag="VF089_05945"
     CDS             1360..1587
                     /locus_tag="VF089_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173538.1"
                     /translation="MHAISNRIDPDELRHRREVINRTAEYIRNERLGLEQNAPWMDAG
                     VWRRRISLLESLAAWYDKETAKINEALQRLR"
     gene            complement(1800..2306)
                     /locus_tag="VF089_05950"
     CDS             complement(1800..2306)
                     /locus_tag="VF089_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173539.1"
                     /translation="MEEVSDLSMLEELMHRPEGISAVEKLLNEFEAHERDEEKSVEEY
                     RKAFAGVKDPATRFIMQMIISDEEKHRAVMHAMASTLKGSLNWSQPAASLEGAPDEAE
                     IKKKLTAVTEEFIDVEKTGIKEYKKLLKGSSGYYQGLFQVLIEAMIRDSEKHVEMLEY
                     LSRRLKSA"
     gene            2832..3293
                     /locus_tag="VF089_05955"
     CDS             2832..3293
                     /locus_tag="VF089_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6173540.1"
                     /translation="METVRKILAPTDLSELSCIGLRRALEMGRLRGAEVIVYYVIDLA
                     DHWAKPEHSGPVREMLEYHKQSLDKFLREKFPEEMNLTEVRQMVEFGGAVDSIVEKAE
                     AEQVDRIVMSTHGRTGIDHLLIGSVSEKVIARAPCPVLIMPAHRRKIARAA"
     gene            3604..4107
                     /locus_tag="VF089_05960"
     CDS             3604..4107
                     /locus_tag="VF089_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173541.1"
                     /translation="MNTSAPFDPLLRFAEIERLVSKIYYRFSHLFLTRQELRDFWWEM
                     AREEEQHASILGACRDLILNYDDESLDPMISSDSADRVAARLQSLLDQGTPTLAIEDA
                     FRIALEIENSEIDVIYGKLLELGGPQIGKTMENLGVPAAVQRQKLKAALRRFCSNPEL
                     VQAAERL"
     gene            4263..5207
                     /locus_tag="VF089_05965"
     CDS             4263..5207
                     /locus_tag="VF089_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6173542.1"
                     /translation="MDFQLNNIIGKSKAIQDVLELVKKVANTQATVLITGESGTGKEL
                     IALALHALSDRREKLFLPVNCGALPETLLESLVFGHVKGSFTGAFANQEGLFEKARGG
                     TIFLDEIGEIPQHLQVKLLRALEAKEILPLGSTTPRRIDVRVLAATNRNLNNEVEAGR
                     FREDLYYRLNVMEIHIPPLRERPEDIPELIEHFIKRHNPDLKRNFQGIDEAAVRVLMT
                     LPWKGNVRELDNVIEHTMILAEGDRIMLKNLPGAIIASSQTHPAFTFNLRDALRHFEK
                     QHIVRALDQANQDRKEAARLLDISLSSLYRKIEELEIS"
     gene            complement(5382..6311)
                     /locus_tag="VF089_05970"
     CDS             complement(5382..6311)
                     /locus_tag="VF089_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6173543.1"
                     /translation="MESPGLTRFAWLSISAAMLTIALKALAYFVTDSVGLLSDALESL
                     VNLAAALMALAMLIVAARPPDEIHAFGYSKAEYFSSGAEGALILLAAGIIAWTAVPRL
                     ITPQPLEQLGIGLGVSIVAAALNFFVARHLLSGSKQYRSITLEADAQHLMTDVWTSAG
                     VVAGIAVVAVTGWTRFDAVIALCVAVNILFTGVRLLRRSLLGLLDTALGHTEQELIKE
                     ILSRYVSQGLHYHALRTRQAGVRSFISFHVLVPGEWTVQRGHDLVERVERDIRAAVPG
                     ATVFTHLEPINDPAAWQDLTLDRSDDARRSKCN"
     gene            complement(6644..>7420)
                     /locus_tag="VF089_05975"
     CDS             complement(6644..>7420)
                     /locus_tag="VF089_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX6173544.1"
                     /translation="EAQSGCLLSARLRPGNASASEGITGELNRLVPLLKRRFPKSQIS
                     YRADAGSATPEIYATLESLQVLYAIGIGTNAVFQRKTDRWLKRAKRKYARRGKTVRIF
                     YSFRHRARSWPKHRKIVVKIEVGPLGTNLRFVVTNRAGRAEQIFNHYDDRGQCENRIK
                     EFKRDLHSDRLSCHRYRANAFRLQLHALAYQLLVLFRRHALTQTHLAHVRLETLRVKL
                     FKVGARFVQSARRLWFHLSSSWPGRDLFVEVWQRLKPAPS"
BASE COUNT         1777 a         1923 c         2017 g         1703 t
ORIGIN      
        1 tgttgaaagc gagggcgtag ttggcgaaag aaagctggcc cagagcttcc atagacggca
       61 cctgcaggaa aggcgtgagc gaggaccaga gcaatgctat catcggcagg agctgcaaaa
      121 acggcaagga cagcaaaagc agagggccga cgaagtgcca cttaccgaga tctttgcgac
      181 gcggatttgg tgggccgacc cgctgaattc ggataatccg tatacggtgg agtcgaaagc
      241 aaaaaaggtc gtagtcgttc cctacaatgt tccgggctgc aatgactact cgacttacgg
      301 cgcgggacga acgccgcacg ttctgctgca aatcatgaaa gaccagttcg actaccttac
      361 tgggagggcg aacagggcgc gccgaagttt tttgctttta acttgcaccc atttgtttcc
      421 ggcgtgccgt ttcgaaccaa aatcatcgac gagtttattc gctacgccaa gggctttccc
      481 aaggtctggt tcgcgcgccg tattgagatc gccaactggt gcttggagaa ggggtattag
      541 gcgttcaccc gggtacatcg gtgtttgcgg gcatttcgaa aaatcggatc aacaaaggtc
      601 cggtatgagt tcagataatg accggcgcgc caccgcactt aaagctaccg cggctttttg
      661 caaatcagac caaggtagac caatggtttt ttctatacgt cactttccga tatctactac
      721 ttcgcaataa agattagttg cggcccgctg agcgcctggt tggaagcgcg gtgatagcgc
      781 cattcacccg atggcgcaga ggatattggg cagctcgagc gaaaggcgaa ttctcaaaga
      841 agcgccggag agcttacgtt cgaggaagcg caaacgctta tacaaggcat caaagcattc
      901 ggcgcgatgg tatgcccgcg agttatcgaa tgaccggacc aagttaagat ctgtgtcacg
      961 gtcttacccg ctaaaaccta gcgccttctc gcagcgcaaa acgtagcaat ttccccgctc
     1021 ttcaaaaagc tttttcgcac gggtcgggca gttgccttac gtagttctta ggtgatgatt
     1081 gtccggcaaa tgctagtagc tcgatggggt gagaggcggg ctggtatcag ctcgttgagc
     1141 ccaaagggat gggtgcctaa atttattgaa ttcctgacga aggccgaagc tcacacatac
     1201 tttcccaact catcattctt ggcgaactcc cgatcgcagc ggttttgatt gtcgggcctt
     1261 tgtcggggat tgaaaccgct tccgcagaaa gagtttggtt tcgatgcgcg tttgaattac
     1321 tcagctccgt gcagctcgct gagggaaagg agcaggctga tgcatgccat ctccaatcgc
     1381 attgatccag atgagctaag acaccggcgg gaggtgatca accgaaccgc ggagtatatc
     1441 aggaatgagc gccttggctt ggagcaaaat gctccatgga tggacgccgg cgtttggcga
     1501 cgcaggatta gtctccttga gtctctggcc gcctggtacg ataaggaaac ggcgaagatc
     1561 aacgaggcgc tccaacgtct acgttaaaaa ctgtttaagt tacagacact ccggcaggtg
     1621 agtgccggct cactgagaca gacgcgaaag ctatgagatc ggactctact ccgactgtag
     1681 ctcgtagaaa tcgcgcaact cgggcgcggc aaagaagaaa tgagaagcga aaataagttt
     1741 tgcagacgag ccgtccgatc tccgcaaagg ttccagcgga gcgaacggct cgggtgaact
     1801 cacgctgatt ttaacctccg gctaagatac tcgagcattt cgacgtgctt ttccgagtcg
     1861 cggatcatgg cctcgatcag aacttggaag aggccctgat agtagcccga gcttcccttc
     1921 aggagctttt tgtactcctt gatcccggtc ttctcaacgt ctatgaactc ctcggtgacg
     1981 gcagtgagtt tcttcttgat ctctgcctcg tccggggcgc cctcaagact cgcagcgggc
     2041 tggctccagt tgagactacc cttcaatgtc gacgccatcg cgtgcattac ggctcggtgc
     2101 ttttcctcat ccgaaatgat catctgcatg atgaaacggg tagccggatc tttcacgccg
     2161 gcgaaagcct tcctgtactc ctcgaccgat ttttcttcat ccctttcgtg agcttcgaat
     2221 tcgttgagca acttctccac cgccgagata ccctcgggtc tgtgcattag ctcttccagc
     2281 atgctcaggt cagaaacttc ttccatgact gtcctccctt cctttcaatg aaaaaatttg
     2341 aaaaagtcgt cgcgcactcc gagaaaatct atgttttctt tgagaacagg atactcttgt
     2401 ttgcatggaa tatctttgac tcggtgtcgc gttaagaaca cctctctcgc cttcagaacc
     2461 aacatcgtat tccttcgtct tctcggctgg tcctcacggg agctgacggt attctcaaac
     2521 atcagtccat cggcttcccc gatgtatcca aggcctaccc ttagagaatc ttgtgacatt
     2581 agctagacca gatagcaagt gagctttcag cctttggagg agcacaggct gtgccaagca
     2641 ctcagtggaa aatgtttggc aatccttaag tcaccccgga tagccgcgcc ggttttccct
     2701 ccgataagaa aaagtttgtc tcaaattcta ctacctgcga aaaactcgtc acctttcagg
     2761 tggattcctt tgtgattcga tggcggcatg ttccttgcgc ttgtacttgg ctatcgacaa
     2821 aggaggacac aatggagaca gtaagaaaaa ttcttgcgcc gacggatctc tcggagcttt
     2881 cctgcatagg actgcgacgc gcgcttgaaa tgggacgatt gcgaggcgca gaggtaattg
     2941 tatattacgt cattgactta gccgatcact gggccaagcc cgagcattct gggccggttc
     3001 gtgagatgct ggagtaccat aaacagtcgc tggacaagtt cctgcgcgag aagtttccgg
     3061 aggagatgaa tctaaccgaa gttcggcaga tggttgagtt cggcggcgcc gtcgacagca
     3121 ttgtcgaaaa ggcggaagct gagcaggtgg acaggatcgt tatgtcaact cacggccgaa
     3181 ctggcattga tcacttgctg atcggcagcg tttccgaaaa agttatcgcc cgcgctccat
     3241 gcccggtcct gatcatgccg gcgcacaggc gcaagatcgc aagggctgcc taattggccg
     3301 taagttatct gcatgatggc ggtaaataag tgcttagatc gacccgctgg aaagccagtc
     3361 tcgactcgaa cgccgaagca cacggtttgt agttcaatcg gcgaacttcc gctgccacgg
     3421 gactgagctt aggcgcctta atggggcggt ggcgtttcgg cgggtgataa actgaaatcg
     3481 gctatgtggc tggatcggcc tctacgactc ggatgctgtt tgctattgca atatggctag
     3541 ttgcacgact cgattccgag aagcatattg agccatttca atttctgata gaatctagaa
     3601 gccatgaata cgtcggcgcc cttcgatccg ctgctgcgct ttgccgagat cgaacgactg
     3661 gtatcaaaaa tttactaccg gttttcgcat ctctttctca cccgacagga gctgcgtgat
     3721 ttctggtggg aaatggcgcg agaggaagag cagcacgcga gtattctggg cgcgtgccgg
     3781 gacctcattc ttaactatga cgacgaatcc ctcgatccga tgatcagcag tgacagtgcc
     3841 gatagagtag ccgctcgact gcagtctctt cttgaccaag gtacaccgac gctcgccatt
     3901 gaggatgcct tcagaatcgc actagaaata gaaaactccg agatcgatgt gatctacggc
     3961 aaactattgg agctgggcgg tccgcagatc ggcaaaacga tggagaatct gggcgtcccc
     4021 gcagccgtgc aaagacaaaa gctcaaggcc gcactgcgcc gcttctgttc caatccggag
     4081 ctagttcagg cggccgagag actctgagtc cgagttccaa ttccctccct ccttgacaca
     4141 ttcatcgtac gcttttatct tgccgatggc gtgcggccgg cattttgtaa gagaggattc
     4201 gcgcgcaaag aacgcgattc gcgtgacatg ccgatgactg ctacatccgg cttaaaaggt
     4261 caatggactt tcaattgaac aacatcatcg gcaaaagcaa ggcgattcag gacgtcctag
     4321 agctggtcaa gaaagttgcc aacactcagg ccacggttct catcaccggc gaaagtggca
     4381 ccggcaaaga gctgatcgct ctggcgctcc acgcgcttag tgatcgccgc gagaaacttt
     4441 ttctgccggt caactgcggt gcattaccgg agacgctgct ggaaagtctt gtttttggcc
     4501 atgtaaaagg ttctttcacc ggcgctttcg ccaatcaaga aggcttgttc gaaaaggcca
     4561 ggggtggaac catttttctc gacgaaatcg gcgagatccc gcagcacctc caagtcaaat
     4621 tgctcagagc tttggaagcc aaggaaattc ttccgctggg gagcacgaca ccgcgccgga
     4681 tcgacgttcg cgttttggcg gcgaccaatc gaaacttaaa taacgaagtg gaagccggtc
     4741 gctttcgtga agatctctac tatcgtctca atgtcatgga aatccatatt ccgcctttac
     4801 gggagcggcc ggaggatatt cccgagttga tcgaacattt catcaagcgt cacaatcccg
     4861 acctcaaacg gaactttcag gggatcgacg aagccgcggt gcgcgttctc atgaccctgc
     4921 catggaaagg caacgtacgc gagctcgaca acgtcatcga gcatacgatg atccttgccg
     4981 aaggcgaccg aatcatgttg aagaatctgc ccggcgccat catcgccagt agtcaaaccc
     5041 atcctgcttt cacattcaat ctcagggatg cgttacgaca tttcgaaaaa cagcacattg
     5101 tgcgggctct ggaccaagcc aatcaagacc gcaaagaggc agccagactt ctggatatca
     5161 gtctctcgtc actctaccgc aaaatcgaag agctggagat tagctgagaa gctttttcga
     5221 ctcgaccatt gtcttgatcg agacagattg actgatatcg caagattagc gttctacagc
     5281 gaattttcag tggcatcctc gttgcggatc agcggcggga ggttgtgact gctgtggcgt
     5341 cgagacgaag tcatcgacaa gccgatgatt gattgtgcct tttagttgca cttgctgcgt
     5401 cttgcgtcat cgctccgatc aagggttaag tcctgccaag ctgccggatc attgatcggc
     5461 tcgaggtgag tgaagaccgt tgcaccggga accgccgcgc gtatatcgcg ctctactcgt
     5521 tcgaccagat catgcccgcg ctgaacggtc cattcccccg gcacgagcac atggaaggaa
     5581 atgaacgacc gcactcccgc ctgccgcgtg cgcagcgcat gatagtgaag accctgacta
     5641 acatatcggc tgaggatttc ttttataagt tcttgctcgg tgtgtcccaa cgccgtgtct
     5701 aaaagtccaa gcaacgagcg gcgtagcaat cgcactcccg tgaataaaat attgacagcg
     5761 acacacagcg cgatcaccgc atcaaatcgc gtccaaccgg tcacggcgac gaccgcgatt
     5821 ccggcaacga caccggccga agtccagaca tcggtcatca ggtgctgggc gtccgcttcc
     5881 agtgtgatgg agcgatactg tttgctgccg ctgagcaaat gccgggcaac gaaaaaattg
     5941 agcgccgctg ctacgatcga tacgccaaga ccaatgccga gttgctccaa tggctgtggc
     6001 gtaatcaatc gtggcaccgc cgtccaggcg atgatgcccg ccgcaaggag aatcagcgcc
     6061 ccttcagcgc cgctggaaaa atactcagct ttgctgtagc cgaaggcgtg aatctcatct
     6121 ggcggtcgag ccgcaacgat cagcattgca agagccatga gagccgcagc cagattcacc
     6181 aatgactcca atgcatccga caaaaggccc acggaatcgg tgacgaaata cgccaatgcc
     6241 ttcaaagcaa tggtcagcat cgcggcgctg atcgagagcc atgcgaagcg agtaagaccg
     6301 gggctttcca tttaacttag acgaattcta cgggcatcat actatgcttc gtctggcgag
     6361 ttctcgagga aacctcttat gaagaaggag tcgtggttga tgaagctatt gacgagaccg
     6421 ctagcccgca ttattcatag ttttgaaatt tgtcggtaaa agttttcatt ggtcgctcgg
     6481 tatctttaat gcgtgtgatt tttttaactt tgcctaatcc aggagaagag aagctgcagt
     6541 ccaaagcgcg ctgggcgagg gaacaacagt tttggggaaa cacctcgacg tcttgtctgt
     6601 gggatggcca tcaggggcaa gatttagtgg cagcgagcag aacttaacta ggtgcaggct
     6661 taagtctttg ccagacctcc acgaacagat cgcgtcctgg ccaactcgaa gagagatgaa
     6721 accataggcg gcgagcgctt tggacaaagc gggcgccgac tttgaacagt ttaactcgta
     6781 gggtctccaa ccgaacatga gcaaggtgag tttgggtaag tgcatgccga cgaaagagca
     6841 ccagcaactg gtaagcgagt gcgtggagtt gaagccgaaa agcattggcg cgataacggt
     6901 ggcacgatag ccggtcggag tgaagatcgc gcttgaattc tttgatccgg ttctcgcact
     6961 gtcctcggtc atcgtagtgg ttaaagattt gttcggctct gcccgctcgg ttagtgacga
     7021 caaagcgcag gttggtgccc agagggccga cttcgatttt gacgacaatt tttctgtgtt
     7081 tgggccagct tcgggctcgg tgtctaaagc tataaaaaat tctgacggtt ttgcctctac
     7141 gagcatactt acgcttggct cgtttaagcc agcggtcggt ttttcgttga aacaccgcgt
     7201 tggtgccgat accgatggca taaaggactt ggagagactc caacgtggca taaatttccg
     7261 gagtggctga gccggcatcg gctcgatagc tgatctggct tttagggaag cgccgcttaa
     7321 gcagaggaac caagcggttg agctcaccgg tgatcccctc tgaagccgag gcattgccgg
     7381 gacgaagtcg ggccgagagc aaacagccgc tttgggcttc
//
LOCUS       DASUTG010000261         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1079712, whole genome shotgun sequence.
ACCESSION   DASUTG010000261 DASUTG010000000
VERSION     DASUTG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079712"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF089_05980"
     CDS             <1..489
                     /locus_tag="VF089_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173545.1"
                     /translation="NSSDAFLMLRAQTVGVSTIEIFLVVAAFSLVVALTSTKGGELSD
                     LFGRRGLIITGWLIYAVIYLGFAFASERWHVWILYAGYGLYYGAFLGASSAVVADLVP
                     EHLRGTAYGIFNAALGVAALPASVIAGVLWDWYGPAAPFLFGAALAVMATIGLMKIPL
                     KR"
     gene            944..1585
                     /locus_tag="VF089_05985"
     CDS             944..1585
                     /locus_tag="VF089_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6173546.1"
                     /translation="MNEIDPKAQTDLDQLKKEALSFVWIGEIWNLLEAGVALWSGVAA
                     GSVALIGFGLDSILELAAGAILIWRLQTEWQDNDEESTAERKAHKLVGITFFVLAGYI
                     LVQSIATLLGYYPEPQETTAGLILIVASAIVMTILYFKKMAIAEKIGSRALRAEAKET
                     LVCDLQDLTVLVGLGANFLFGWWWADPVAALGLIPFLVKEGWEGVMGEDDDDD"
     gene            1812..2783
                     /locus_tag="VF089_05990"
     CDS             1812..2783
                     /locus_tag="VF089_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173547.1"
                     /translation="MRLSRVGWILFALVLLAVAAHETSAQDRISLGITTKTGSTGLPF
                     VIAEEKGFFKSEGLNAVSVVMQNQVVVNGVVARQVDYGGTFSNFVGAALSGLPVRIVM
                     AVMDGSDHYLVTSPNIKRVEDLKGKKFGISSFGGTPHSEAVMILRKFGMNPEKDVTFL
                     QIGGSSSRYAALDSGSIDAAMLVPPFNKLAAKRGFNEILSFNEIMSIPLGGLTVHTQR
                     IKEKPDEIVKMIKAMLKGVNYIRDNQAEILSIMASKWGIKEADVREGIYRDIVGIYTR
                     NGIASDETMKNVIQLVRDTRKSKDDLGLSDIVDWSFAKKAQAELKIR"
     gene            complement(2905..>3249)
                     /locus_tag="VF089_05995"
     CDS             complement(2905..>3249)
                     /locus_tag="VF089_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173548.1"
                     /translation="VKGSFLREDRDRVKRFVRAYSEAVHAIKNDRERTLKVFAKRMRI
                     DDAETLQSTYNYFAPRFSLPPRVDLTGVRDTIRFYAEHNPELKNRTAEDFIDHSLLDE
                     IEKEGFFKKLGS"
BASE COUNT          757 a          781 c          917 g          794 t
ORIGIN      
        1 aactccagcg atgcctttct catgctccgc gcccagaccg tgggcgtttc caccattgaa
       61 atatttttag tcgtcgctgc gttcagctta gtcgtggctc tgacctcgac aaagggcgga
      121 gagctttctg atctttttgg caggcgcgga ttgatcatta ccggctggct gatctatgcg
      181 gtgatttatc tcgggtttgc cttcgcatcg gaacgttggc acgtctggat tctttatgcg
      241 gggtacggcc tttattatgg agcttttctc ggcgcctcga gcgcagtggt agccgactta
      301 gtacccgagc acctgcgcgg caccgcctat ggaatcttca atgcggcact gggcgtggcc
      361 gccttgccgg caagcgtgat cgcgggagtg ctctgggact ggtacggccc ggccgcgccg
      421 tttctgttcg gcgccgcact tgcggtcatg gcgacgatcg gtctgatgaa gatcccgctg
      481 aaacggtgag gcggcgtttc tgaccaatta ttacgactct accggtgacc gagaacaatc
      541 tatacggagg cacatggcgt ttactatgat cgacgccttg ggccgatcat cagcagcggc
      601 gagaaggcga tccgaatcag cggctatggt tgccttgacg cacattctat agagactcgt
      661 gttcctagcg aaaatttttc tcatcgtgtc agtgatcata actgtccgta cgctcaccgt
      721 cgtcacgcgc atgagcaggt cgatgacttt ttctttgtag tcagcgaagc ggtaggtgtt
      781 gaacttttcg ccgatggtcg ggtctttggg tttcttttct ttgtactggt cgagaattgt
      841 cgaagggact gcagctggac cctcgggacg gcgacaaatt gtcgtacagt ggtgaatttg
      901 ggcctaagaa caagggcgca caaggattga acgagcggaa gctatgaatg agatagatcc
      961 aaaagcgcaa acggatttag accagctcaa gaaggaggcg ttgtcgttcg tatggattgg
     1021 tgaaatctgg aatctgctgg aagcgggtgt ggcgctgtgg tcgggagtcg cagccggaag
     1081 tgttgctttg atcggatttg gcctcgacag cattctcgaa cttgcagccg gagcgatctt
     1141 gatatggcgg ctgcaaacag aatggcagga taatgacgaa gagagcacgg cagaacggaa
     1201 ggcccacaag ctcgtgggaa ttacattttt cgtactcgcc ggttacatct tagttcaatc
     1261 tatcgcgacc ctcctcggtt attatccaga gcctcaagaa accacagcgg gtctcatcct
     1321 gatcgtcgcc agcgcgattg tgatgacaat cctttatttt aagaaaatgg ctattgcgga
     1381 gaagattggt tctcgtgcgt tgcgagcaga agcaaaagaa accctggtct gtgatttaca
     1441 ggatctgact gttctcgttg gtttaggagc taattttctt ttcggctggt ggtgggcgga
     1501 tccggtagct gctctgggat tgataccttt cttagtaaaa gaaggttggg aaggggttat
     1561 gggcgaagat gatgacgacg attaaaaact tctacgtcaa gccgcccgtt acggttcatg
     1621 gacactgctc gttaaaccaa cttatgctct tatgccatac tgtcgtttat gacccacgca
     1681 aatccaggag gtgagtccgc ttgacacaat gctaacgcta aattaagcgt aaaatcgcaa
     1741 cgatgaaggc aggcgtaagc acaggtgcgt gcttgcagcc ggggagctaa gctaacgcat
     1801 tggaggtcac aatgagattg agtcgagtcg ggtggattct tttcgcacta gttttgctcg
     1861 ccgtggcggc gcacgaaacc agcgcgcagg atcgtatcag tctcgggatc acgaccaaga
     1921 ccggcagcac tggcctgccg ttcgtcatcg cggaggagaa aggattcttc aaaagcgaag
     1981 ggctcaacgc ggtttcggtc gtgatgcaga accaggtcgt ggtcaatggt gttgtcgctc
     2041 gacaggtcga ttacggcggg accttttcga acttcgttgg agcggctctc tcgggccttc
     2101 ccgtgcgcat cgtcatggcg gtgatggatg gctcggacca ctacctggtg acgagcccga
     2161 acataaaaag ggttgaagat ctcaaaggca aaaaattcgg catcagcagt ttcggcggca
     2221 cgccgcacag cgaagcagtc atgattctgc ggaagttcgg catgaatccg gaaaaagatg
     2281 ttacgtttct ccagatcggc ggcagttctt cccgttatgc cgcattggac agcggctcca
     2341 tcgacgccgc tatgctggtg cctcctttca acaagctggc tgcgaaacga ggattcaatg
     2401 agattctttc attcaatgag atcatgagca tcccgttggg gggcctgacc gttcacaccc
     2461 aaagaatcaa agaaaaaccc gatgaaatcg tcaagatgat caaggccatg ctgaaaggag
     2521 tcaactacat ccgagataac caagcggaga tcctttcaat catggcaagc aaatggggca
     2581 tcaaagaggc tgatgtgcgc gaaggcattt accgcgatat cgtcgggatc tacacccgaa
     2641 acggcatcgc ttcggacgag acaatgaaaa acgttattca actagtgcgc gacacgcgaa
     2701 aatccaagga tgatttgggg ctatccgaca tcgttgactg gagctttgca aaaaaagcac
     2761 aagcggaatt aaagattcgc tgaatgatct gtgcgggttc aagttgttca aatagttcaa
     2821 gccgttcaag tcgtttcaga tgaactcgcg atccggacgg tttgaactgc ttgaacgact
     2881 tgaagttttt gaaccagccg tgcgttacga gcccagcttc ttaaagaagc cctctttttc
     2941 gatttcatcg agcagcgaat gatcgatgaa atcctccgcc gtgcggttct ttagttctgg
     3001 attatgctcg gcatagaatc ggatcgtatc ccgcacaccg gttaaatcta cccgcggcgg
     3061 cagtgagaaa cgcggcgcga aataattata ggtcgattga agtgtttccg cgtcatcaat
     3121 gcgcatgcgt ttagcgaaca ccttcaaggt tctctcgcgg tcatttttga tcgcgtggac
     3181 ggcttccgag taggcgcgga cgaagcgctt gacccgatcg cgatcttccc tgagaaaact
     3241 gcctttgac
//
LOCUS       DASUTG010000262         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1227464, whole genome shotgun sequence.
ACCESSION   DASUTG010000262 DASUTG010000000
VERSION     DASUTG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227464"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..2461)
                     /gene="gyrB"
                     /locus_tag="VF089_06000"
     CDS             complement(32..2461)
                     /gene="gyrB"
                     /locus_tag="VF089_06000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX6173549.1"
                     /translation="MAQSKPPQQDDYKADNIKVLEGLEAVRKRPGMYIGDTGERGLHH
                     LVYEVVDNSIDEALAGHCDNIEVTIHIDNSVTVIDNGRGIPVDIHSTEGVSAAEVVLT
                     KLHAGGKFDKSSYKVSGGLHGVGISVVNALSEVLEVEIKRDGRVYQQSYRRGVPQDRL
                     KEVGKSKERGTKVTFTPDTEIFETVEFSFDILSQRLRELAFLNSGVKITIEDERSQKK
                     HEFFYKGGLLSFVEHLNRAKAAIHPKVVHFAGEKEGVEVEIAMQWNDGYSENVFSFAN
                     NINTAEGGTHLIGFKSALTRTVNSYAIEIGQLKKDETNLQGEDVREGLTAVVSVKVPE
                     PQFEGQTKTKLGNSEVKGIVEALVNEKFSSYLSEHPADAKKIISKGVEAARVREATRK
                     AKDLARRKGALDSGSLPGKLADCQERDPALSEIYLVEGDSAGGSAKQGRDRRNQAILP
                     LKGKILNVEKARFDKMLSSQEIRLMITALGTGVKEDYDSAKLRYHSIIIMTDADVDGS
                     HIRTLLLTFFYRQMPDLVEKGHLFIAQPPLYKVKRGKQERYLKDEATLEDHLIELGTE
                     NIRVRATNDGTGLTGQALQGFVKKVARWEKLMALITRKRKNRVVIEGLLLEEQFSEET
                     LKDEKALRGLESRLVSYINLTAPDQAPVSCALEEDPEHNCFKLVCATRTNGSRSQTVI
                     DREFLISAEYKEIKRLFTELTSIGVPPYAVENGEQKAQLRSLTEVLKYIMEGGKKGQY
                     IQRYKGLGEMNPDQLWETTMNPETRILLQVRIEDAVEANDIFSTLMGDEVEPRRKFIE
                     DHALTVKNLDI"
     gene            2696..3406
                     /locus_tag="VF089_06005"
     CDS             2696..3406
                     /locus_tag="VF089_06005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX6173550.1"
                     /translation="MKSIVAAFKFLTILGRLSRFQPSAPMVGKAAAFFPVVGLVLGVS
                     LALLARTLENYLDAEILSTTLVGFLIVATGGLHLEGLKKTFDAPELNQSRAGNESSSS
                     AVATLAILFVILFKIKSIDILEEKLNLALLLAPVLARWAMLIFIYGSDRRSEGQASII
                     AANVKFWHVVLATVATLAPATYFLGRTALWIGLSLSILALLCRALFHKRTGVLTHDNY
                     GAVVEASEVLSLVLMATL"
     gene            3424..4299
                     /locus_tag="VF089_06010"
     CDS             3424..4299
                     /locus_tag="VF089_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX6173551.1"
                     /translation="MEVFTPVYPVVPVFVLITIGFCFAHLKKLSLLPITELIVYLGTP
                     SLVFTSLASRPIFFNDILILAAGVLLIFTGVGFLIGLYFSFFRFSSRGFALPVLFMNA
                     GNMGIPLALFAFGEPGLQRATLMFVIITFLQYSLGIYLLSGRGNWTEIFRLPLIYATI
                     AGLILNLNRLTIPELLFQPISMLGQATIPIMLVSLGYRLHEFQSFKWRHAFGGALVRI
                     FGGFTTAAIVVQLIAAEGVNRQVLLLYGALPAAVINFVLTEKYGKDPELAASIVVLST
                     FISVVTIPIVFWLIL"
     regulatory      4376..4578
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          991 a         1256 c         1144 g         1246 t
ORIGIN      
        1 agaacagtgc cggacggaac ggcgctccga actagatgtc gaggtttttc accgtgaggg
       61 cgtgatcttc gataaatttg cgccgaggtt cgacttcatc acccatcaaa gtcgaaaaaa
      121 tgtcgttggc ttctaccgca tcctcaatcc taacctgcaa gagaatccgt gtttccggat
      181 tcatcgtggt ttcccaaagt tgatccggat tcatctcgcc aaggccttta taccgctgga
      241 tatactgccc ctttttgccg ccctccatga tgtacttaag aacttcggta agagagcgaa
      301 gctgagcttt ctgctcgccg ttttccaccg cgtaaggcgg aacgccgata ctggtgagct
      361 cggtgaacag tcttttaatt tctttatact cagcgctgat aagaaactcg cgatcgataa
      421 cagtttgtga ccttgagccg tttgttcggg tggcgcaaac aagcttaaag caattgtgct
      481 ccgggtcttc ttccagcgcg cacgagaccg gcgcttggtc cggagccgtg aggtttatgt
      541 aagagaccag gcggctttcg agaccgcgca gcgctttctc gtcttttaag gtttcctcgc
      601 tgaactgctc ttccaatagg agcccttcga tgacaactcg attttttctt tttcgcgtaa
      661 tcagagccat cagcttttcc caacgggcaa cttttttcac aaaaccctgt agcgcctgac
      721 cggttaaccc agtgccgtca ttggtggcgc gcacgcgtat attttcggtg ccgagctcaa
      781 tcaaatgatc ttccagggtt gcttcatctt tgagataccg ctcctgcttg ccgcgcttca
      841 ctttgtaaag cggcggctga gcgataaaca gatggccctt ctcgaccaaa tcgggcatct
      901 ggcgataaaa aaacgtcaat agcaaagtgc gaatatgcga gccgtcgacg tccgcatcgg
      961 tcatgataat gatcgagtgg tagcgaagct ttgcagaatc gtaatcttct ttaacgccgg
     1021 tgccaagtgc cgtgatcatc aagcgaattt cctgcgagga cagcatcttg tcgaagcgcg
     1081 ccttttcgac gttgagaatc ttccctttga gaggaagaat cgcttgattg cgccggtctc
     1141 gtccctgctt cgcggaaccc ccggcggaat ctccctccac gagataaatc tcgctcagcg
     1201 ccggatctct ctcctgacaa tcggctagct ttccaggcaa tgatccagaa tctaaggcgc
     1261 ccttgcgccg tgccaaatcc ttggccttgc gggtcgcctc ccgcactcga gcagcttcaa
     1321 cacccttgga gataatcttc ttggcgtcag cggggtgttc gctaagatag ctggagaact
     1381 tctcgttgac gagcgcctca acaatgcctt tgacttcgct attgcccagt tttgttttgg
     1441 tttgtccttc gaactgcggc tccgggactt tgacgctcac caccgcagtg agcccctcgc
     1501 ggacgtcttc gccctgcaga tttgtttcgt cttttttcag ttgcccgatt tcgatggcat
     1561 agctgttcac agtgcgcgtc aatgcggact taaagccgat cagatgggtt ccaccctcag
     1621 cggtgttgat gttattcgca aatgaaaaaa cattctcaga atagccatcg ttccactgca
     1681 tggcaatttc gacctcaacg ccttcctttt cgccggcgaa gtggacgacc tttgggtgaa
     1741 tagcggcttt ggcgcgattg agatgttcga caaaagagag aagaccccct ttgtagaaaa
     1801 actcgtgctt cttttgactg cgctcgtctt caatagtgat ctttacgccg ctatttaaaa
     1861 acgcgagctc gcgcagcctt tgtgaaagaa tgtcgaagct aaactcaacg gtttcgaaga
     1921 tctctgtgtc tggcgtaaac gtaaccttgg taccgcgctc cttagatttt ccgacttctt
     1981 taagcctgtc ctgcgggacg ccgcgtcgat agctctgttg atagaccctg ccgtcgcgtt
     2041 tgatttcaac ctcgaggact tccgacaacg cgttgacaac tgaaatgccg acaccgtgaa
     2101 ggccgccgga aaccttgtac gaggatttgt cgaactttcc gcctgcatgc agtttggtga
     2161 gcacgacttc agcagccgaa actccctcag tcgaatggat atccacggga ataccgcgac
     2221 cgttatcaat cacggtcacg ctattatcaa tatggatggt aacctcgatg ttatcgcagt
     2281 ggccagccaa agcttcgtca atagagttgt cgaccacctc ataaactaaa tgatgcaagc
     2341 cacgttcgcc ggtgtcgccg atgtacattc cagggcgttt tctcaccgct tcgagacctt
     2401 caaggacctt tatgttatcg gctttatagt cgtcttgctg aggtggtttt gattgagcca
     2461 ttttcacctg atgtcaactg aaatattgct tggaaagttc tggggttaga cccgaattga
     2521 gactgccaac atagtacctc aactgggccc gaaagtaaag ccaaaacggt cgcctaaatt
     2581 gctgatttct tagggaatat tttcacctgc aaggcctccg ggcgggaatc gcggcggcag
     2641 atgtgaattg tcgtgccgtt gacagctccg catctctaag acaaaaggga accgcatgaa
     2701 gagcattgtc gctgcattca aatttctcac tattttaggc cggcttagcc gcttccaacc
     2761 gagcgcgccg atggtgggta aagcggcggc gtttttccct gtcgtcggct tggtgctcgg
     2821 cgtgagtcta gcgctgctcg cgcgaacact tgaaaattat ctggatgcag aaattctcag
     2881 cacgacgtta gtgggttttc tgattgtggc caccggaggc cttcacctgg aaggattaaa
     2941 aaagacgttc gatgcgccag aattgaatca gtccagggca gggaacgagt catcgagcag
     3001 cgctgttgcg actcttgcga tcctcttcgt gatacttttt aaaatcaagt ccatcgatat
     3061 tctagaagaa aagctcaacc tcgctttgct cctagcgcct gtgctagcgc gctgggcgat
     3121 gctgattttc atctacggct cagaccgccg cagcgaaggg caggcaagta ttatcgcggc
     3181 aaacgtgaaa ttctggcacg ttgtgcttgc gaccgttgcg acactcgcgc cggcaactta
     3241 cttccttggc cgcaccgcgc tttggatcgg actttctctt tcaattctgg ccctgctctg
     3301 tcgagctctg ttccacaagc gaaccggtgt gctaactcat gacaactacg gcgccgttgt
     3361 cgaagctagc gaagtcttga gcctggtgct gatggcgacg ctctgactct cagaatctcc
     3421 gagatggaag tcttcacccc cgtctatccc gtggttccgg tgtttgtgct aatcaccatc
     3481 ggcttttgct tcgcacattt gaagaaactc agtctgcttc cgattaccga gctgatcgta
     3541 tatctgggaa cgccttctct ggtgtttacc tctttggcta gtagaccgat cttttttaac
     3601 gatatcctta tattagcagc cggcgttctt ttgatcttca ccggtgttgg atttctaatc
     3661 gggctctact tctccttctt tcgattcagc tcacgaggct ttgctctacc cgtgttgttc
     3721 atgaacgcgg gcaatatggg cattccgctg gcactgtttg cctttggcga gcccggcttg
     3781 caacgtgcca cgttgatgtt tgtgatcatt acttttctcc aatattcact aggcatctac
     3841 cttttgagcg ggcgcggcaa ttggaccgag atttttcgtt tgccgttaat ctacgcaacg
     3901 attgcaggtt taatcttgaa ccttaaccgg ctgacgattc cggaactgct cttccaaccc
     3961 atatcgatgc tcggtcaagc aaccattcca atcatgcttg tcagcctggg ataccgcctg
     4021 catgaatttc aatcgttcaa atggcgccac gcctttggcg gcgccctcgt tcggatcttc
     4081 ggtgggttta cgacggcggc tatcgtggtt caattgatcg ctgccgaagg cgtcaatcgc
     4141 caggtattgc tgctttacgg cgccctcccg gcagcagtga tcaatttcgt gctcaccgaa
     4201 aagtacggca aggatccgga gctcgctgcc tccatcgtcg ttttgagcac tttcatttca
     4261 gtggtcacga tcccgattgt attttggttg atcctttgac cgttccgatg cgcccggctc
     4321 atcacactag cattcaatga tcaactatgt tgacaatttc cagctcacca ggtagtttcc
     4381 gcgacatcag tttggcgctc taagatcaac ggaagccggt gtgaatccgg cgcggtcccg
     4441 ccactgtaat cggggtaaga tctttcaccg gcagccactg tcctgtgcca tcgcaaagga
     4501 tgggaaggcg tgaaagatag gcgaggaaag cctctccatc ccgtaagcca ggagacctcc
     4561 tagagccgta taccgaactc tccgacagag agagaaaggt ttctcatagt tcatcccgaa
     4621 tctccgtccc gaactac
//
LOCUS       DASUTG010000263         6128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1524294, whole genome shotgun sequence.
ACCESSION   DASUTG010000263 DASUTG010000000
VERSION     DASUTG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524294"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1748)
                     /locus_tag="VF089_06015"
     CDS             complement(291..1748)
                     /locus_tag="VF089_06015"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX6173552.1"
                     /translation="MAQDVPALQELGKHIFFDNISNPPRQSCSTCHVPENGWTGGVAG
                     INKKGVAVPGANPHTVGGRKPPSNAYATFSPPFGGRPATNPNPFPNPPPATDSFFCGE
                     GSPFLCFGGVFWDGRAEGNATPLVAPPNVTGKGAIPHVGVEIFDTDAQKVAYDGFIGP
                     VADQALGPFPNDVEQNVPDGNDDGLPGAGAVCRHVQSSKYAELFTKAWNEALDCNTSN
                     VAISFKRIALAIAAWQHSGEVNSFSSKRDQGIRNGGSFPLPNFTNQENLGRDLFHGLN
                     DSGLNRLVDDGNGGRTPKNAGCAACHNSHGAASVGNELNQIYSDFAYHHIGLPANFEI
                     ENFDPNDLDAGMLGHTDPSAAFNSANPTATPFAGHVKTPTLRNVDKRRGTGFPKAYMH
                     NGYFKSLEQVVHFYNTATLLRDPLACPAGTTADQAIAKNCWPAPEFDTPNQAGNFGVL
                     GNLGLTTAEEAALVAYLKTLTDTVTPKQPEPYKGG"
     gene            complement(2472..3833)
                     /locus_tag="VF089_06020"
     CDS             complement(2472..3833)
                     /locus_tag="VF089_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6173553.1"
                     /translation="MANGITEQLAIHTTKVRYEDIPSEAIQKAKDCILDQIGVELIGS
                     TLEWNKIAYRYVAEMGGRGESTVVNYGDRVPTLDAAFVNATFGQGCELDDVGFAAGGH
                     PSAATVPVALALGEKQHSTGKEFLAAVVVGCDVMYRLLAAVRPSSGKRGFHSHGIGAP
                     FGAMATAGRLLGLKEGQMLHAFGIAGMHSSGTMEYDQTGGEVKRVIAGIGARSGIQSA
                     MLAQLGLTGPPTIIEGKRGFLKVFADLVEPEEITRDLGSEFKIMRIWFKLYPCVATVQ
                     GVIYTLSKLIEEHRFGADDVEEIRVGISETSLSHGGAIYEPHDTASAQFSLPFSLAIR
                     LLKNDNDLSFYMDPKLWTDPKVLALAKKVKSYADPNAKKDQNYNTTMEVKLTNGKTYK
                     AFEPYPPGSPLNMVSRDVLRAKFRKMARAVLPEDRIDRLIDAVDRLETCNDTGELIPL
                     VVK"
     gene            complement(3904..4920)
                     /locus_tag="VF089_06025"
     CDS             complement(3904..4920)
                     /locus_tag="VF089_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173554.1"
                     /translation="MNRLRKYFIFSFVIVSMSLLAGQLGAQDRDAIIAKAKEEKVFVY
                     YSTTDIRDGTAMIHAFQKKYPFVEPKLLRLGSTQVVVKVLQEHRGGVHLFDVISATSF
                     QFYEIFKEGLFQMYNSPERPAFADDFKDKEGYWVSAYHNASVVSYNTNLVKPQELPKS
                     YDDLLDPKWKGKMLMDSRETEWYASMLQILGREKGLRLMRGLAKQELSFRPGRTLITQ
                     VLASGEAPLAVNNYDHLVQSAKKRGAPVESLPLTPVVSRVTPIALGRYAPHPNIGKLF
                     IDFSLSEEGQKILRGFGRSSARKGIEPDDLQRKGIKLHVSDIGLAKDYARYDKEFKEI
                     FDLK"
     gene            complement(4993..>6128)
                     /gene="tcuA"
                     /locus_tag="VF089_06030"
     CDS             complement(4993..>6128)
                     /gene="tcuA"
                     /locus_tag="VF089_06030"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6173555.1"
                     /translation="ARWLTDMNVKWILSTSTHAVKMGGKIKFPSGRVISVNDGGLGLV
                     EMLFPTAEKNGIEIIYEAKATALIVDPKGRVSGVRVQTAEGLVDFQSRAVVLAAGGFE
                     ANPEMRARYLGAGWDLVKVRGSRYNTGEVLSLALSFGAQAVGHWSGCHAVLVDAEAPD
                     VECAYEHRYSYPYGIMVDINGRRFVDEGEDFFSYTYAKCGREVLRLPWRTAYQIFDGK
                     TRPLLRSEYNRGFHVSADTIEALAKKLPGLDWENVVKTVKAFNDAVNDAPFDPSKHDG
                     KSTSGISPVKSNWAQRLDTPPYYAFPVTCGITFTFGGVGITENGEVKDTSGNVIGGLF
                     AAGEITGGSFFNNYPGGSGLMKGAVFGRLAGTSAAAFAKANPA"
BASE COUNT         1245 a         1753 c         1721 g         1409 t
ORIGIN      
        1 atacctgctg gcatccggcg aggtcagcgc gacgaacttt attgtgtttg ccgccgcgat
       61 tgtaggggta gttgactttg ttggtgaagt tgcccaggag gcgctattag acccattgct
      121 ttaacgactt cggtcttaag acctgcatga ttttgtcctt gagctgctac atgccttcgg
      181 cgatccgcgc ctatcatcag gaacgttgca cgagaagacc ttgcctctcg ctggcccttc
      241 ccgttttcga cgacagctta gtcaacacat tttcgtcaaa gccgatttat ctagccgcct
      301 ttgtagggct ctggctgctt gggcgtcacc gtatcggtca gcgtttttaa gtacgcgacc
      361 agcgctgcct cttcggccgt ggtcagccca aggtttccca gcacgccgaa attcccggct
      421 tgattgggtg tatcgaactc cggagccggc cagcagtttt ttgctatggc ctggtctgcc
      481 gtcgtaccgg ccgggcaagc caagggatcc cgtaacaagg ttgccgtatt ataaaaatga
      541 acgacttgct cgagtgactt gaagtagccg ttatgcatgt aagccttggg aaagccggtg
      601 ccgcggcgct tgtctacatt tcgcaaggtc ggggttttaa cgtgaccggc aaacggggtt
      661 gcggtgggat tggcagagtt gaatgctgcg cttggatcag tatggccgag cattcctgcg
      721 tctaaatcgt tcggatcgaa gttctctatt tcgaaattcg ccggcaatcc aatgtggtga
      781 taagcaaaat ccgagtagat ctggttaagt tcattgccaa cagaagcggc gccatggctg
      841 ttgtgacagg ctgcgcagcc tgcgttcttt ggggtacggc caccgttacc gtcatcgacg
      901 agtctattca gccctgaatc attgaggcca tgaaataggt cacgccccag attctcctgg
      961 ttcgtaaagt tgggcagagg gaaagacccg ccattcctga ttccctgatc gcgcttggag
     1021 ctgaacgagt tgacttcacc ggaatgctgc caggccgcga tggccaaagc gatgcgcttg
     1081 aagctgatcg ccacgttgga agtgttgcag tcgagcgctt cgttccaagc ctttgtgaaa
     1141 agctcggcat acttcgacga ctggacgtgc cgacagacag ctccggcacc gggcaatcca
     1201 tcatcgttgc catcaggaac attctgctcg acatcattcg ggaatggacc gagggcttga
     1261 tcggctaccg gcccgatgaa accatcgtaa gccacctttt gcgcatcggt atcgaaaatc
     1321 tcgacaccaa catgcgggat tgctcctttt cccgtcacgt tcggcggagc aaccaagggc
     1381 gtggcgttgc cttccgcgcg gccatcccag aaaacgcccc cgaaacagag aaatggtgaa
     1441 ccttcgccac agaaaaagga atccgtagca ggcggcggat taggaaaggg attggggttg
     1501 gtcgcaggcc ggccaccaaa cggtggactg aaggttgcgt aggcgttgct gggcggctta
     1561 cgtcctccga cggtgtgagg attggcgcca ggtaccgcca cgcctttctt gttaatgccg
     1621 gcgacgccac cggtccagcc attttccggt acgtggcagg tggaacacga ctgacgcggc
     1681 ggattagaaa tattgtcgaa gaaaatatgc ttgcctagtt cttgtagagc cggcacgtct
     1741 tgggccatgg cagatccatt caacagcact aacaacacac cagcagaaac gcatagtatc
     1801 gcacctttca tgcgagctgt gcttcggtaa cttgttgcgc gatgttcgtg gctctcgatt
     1861 cgcattatca ctcccgggga tctcggcccc tgtttatttg atcatttcgt tttctacgcc
     1921 gctgttgcgc acgcgcggca catcgatgaa ctagcgccga gaatcgagcg ctgctgcagg
     1981 atcatctttg ttctcttaga tggagatttt taccggaacc gcgcctcgcg taccgagcaa
     2041 tccccatacc atggtatgtg aaacgaaaag atttcttcat catatggaga atagtgtcgt
     2101 cgactgctcg cgggcagtaa aaacttttca caatgaatcc gtgacaagct cgatccttca
     2161 gccagtaatt aagaaagcgg tgtgaaattt taaattacga atctgctccg acgagatgat
     2221 caaccgatcc aaattttttt ggagcggcga gtgaaacgag gattttagat ctttgggtaa
     2281 cgaccgtctg actggaattt gagtcaccgt aatgtttgga ataccctgcg cttctcaatg
     2341 ttggatttaa ctcttccggt tcacggacaa tctattgccg acgtgatcgc gacgatcgcg
     2401 cacagatgaa cgtgatgaac gaaagagtat ttcttaagag acgttacggc agcgcgcagt
     2461 tatcccgacg atcacttcac gaccagaggt atcaattcgc cggtatcatt gcaggtttcc
     2521 agccggtcca cggcatcgat caatctgtcg atccgatctt cgggtaatac cgcgcgggcc
     2581 atcttgcgaa acttggcgcg caacacgtca cggctgacca tgttgagcgg cgagccaggg
     2641 gggtagggct cgaacgcttt gtaagtcttg ccattggtga gcttcacttc catcgtcgtg
     2701 ttataattct ggtccttttt cgcgttcggg tcggcgtagc tcttcacttt cttggctagc
     2761 gccagaactt tcggatcggt ccacagcttg ggatccatgt aaaagctcag atcgttgtcg
     2821 ttcttcaaca ggcggatggc caacgagaac ggcaggctga actgcgcgct cgcggtatcg
     2881 tgcggctcgt aaatcgcgcc gccgtgcgac aacgaagttt cggagatgcc gacgcggatt
     2941 tcttcgacgt cgtcggcccc gaagcgatgc tcttcgatca atttgctgag cgtgtagatc
     3001 actccctgga cggtggcgac gcacggataa agcttgaacc agatgcgcat gatcttgaac
     3061 tcactgccga gatcgcgggt gatttcctcc ggctctacca gatcggcgaa gactttaaga
     3121 aatccgcgtt tgccctcgat gatggtcggc gggccggtga gcccgagttg ggctagcatc
     3181 gccgactgga tgccgctgcg cgcgccgata ccggcaatca cgcgcttcac ctcgccgccg
     3241 gtttgatcgt attccatggt gccgctcgaa tgcatgccgg cgatgccgaa ggcatgcagc
     3301 atctgcccct ctttcaatcc cagcagtctc cctgccgttg ccatcgcgcc gaagggtgcg
     3361 ccgatgccgt ggctatgaaa cccgcgcttg cccgacgaag gccgcaccgc cgccagcagc
     3421 cggtacatga cgtcgcagcc gacgaccacg gcggcgagaa attctttgcc cgtgctgtgt
     3481 tgcttttcac ccagcgccag cgccaccggc acggtagcag cgctcgggtg gccgcccgcg
     3541 gcaaagccga cgtcgtcgag ctcgcagccc tgaccgaagg tcgcattgac gaacgccgca
     3601 tccagagtcg gcactctgtc gccgtagttg accacagtgc tttcgccgcg cccgcccatc
     3661 tcggcgacgt agcggtaggc aatcttattc cactcgaggg tggaaccaat cagctcgacg
     3721 ccgatctggt cgaggatgca atctttggct ttttggattg cctcggaagg aatgtcttcg
     3781 tagcgcactt tcgtcgtatg gatcgccagt tgttcggtaa tgccgtttgc catttttcct
     3841 ctcgtgtatg ccggatgatc ctgctgggat agtcgctgcg cgtgatgaca ccgcaacctt
     3901 tgatcatttt aaatcgaaaa tttctttgaa ctctttgtcg tagcgcgcgt aatccttcgc
     3961 caggccgatg tcgctgacat ggagcttgat gccttttctc tgcagatcgt ccggttcgat
     4021 gcctttgcgc gccgagctgc ggccgaagcc gcggagaatt ttctggcctt cctcggaaag
     4081 tgaaaagtcg ataaacagct tgccgatgtt ggggtgcggc gcgtagcgcc cgagcgcgat
     4141 cggggtaacc cggctgacga ccggcgtcag cggcagagac tccacgggtg cgccgcgctt
     4201 cttagccgac tgcacgagat ggtcgtaatt attgaccgcc agcggcgcct cgccagaggc
     4261 caatacctgg gtgatcagcg tgcgtcccgg gcgaaagctc aactcttgtt tggccaggcc
     4321 gcgcattagg cgcaggcctt tttctctgcc gagaatttgc agcatgctcg cgtaccactc
     4381 ggtctcgcga ctgtccatga gcattttgcc tttccacttg ggatcgagca gatcgtcgta
     4441 gcttttgggg agttcctgcg gcttcaccag attggtgttg tacgacacca cgctggcatt
     4501 gtggtaagcg ctgacccagt agccttcctt gtctttgaaa tcgtcggcaa atgccggccg
     4561 ctcgggcgag ttgtacattt gaaacagacc ttctttgaag atctcataga actgaaaact
     4621 ggtcgcggag atgacgtcga acaagtggac gccgccgcgg tgctcctgca gcaccttgac
     4681 gaccacctgc gtgctgccga gacgcagcag cttgggctcg acgaacggat atttcttctg
     4741 gaacgcatgg atcatcgccg tcccgtcgcg gatgtccgta gtgctgtaat acacgaacac
     4801 tttttcttcc ttggccttgg cgatgatcgc atcgcgatcc tgcgccccga gctgacccgc
     4861 gagtaaagac atcgaaacaa tgacaaaaga aaaaatgaaa tattttcgta ggcgattcac
     4921 gtgcgtttct ccctcaactt taaaccaagc gatgacattg aactttgaac gttgaacgcg
     4981 ccagccggcg ctctaggctg ggttcgcctt ggcaaaggca gcagcgctgg tgcccgccag
     5041 gcggccgaac acagcgccct tcatcaagcc ggaaccgccg gggtaattgt tgaaaaagga
     5101 gcccccggta atttcccccg cggcgaacag gccgccaatc acattaccgc tcgtgtcctt
     5161 gacctcaccg ttctcggtga tgccgacgcc gccgaacgtg aacgtgatgc cgcaggtcac
     5221 cgggaacgca tagtagggtg gtgtgtccag gcgctgcgcc caattggact tcaccggact
     5281 gatgccgctc gtggatttgc catcgtgctt cgacgggtcg aacggtgcgt cgttgaccgc
     5341 gtcgttgaac gccttgaccg ttttcacaac gttctcccaa tccaaacccg gcaacttctt
     5401 cgccaacgcc tcgatcgtat cggcgctgac atgaaagccg cggttgtact cggagcggag
     5461 cagcgggcgg gttttgccgt cgaaaatttg ataggccgtc cgccaaggca ggcgcagcac
     5521 ttcgcggccg cacttggcgt aggtgtagct gaaaaaatcc tcgccttcgt cgacgaagcg
     5581 cctgccgttg atgtcgacca tgattccgta gggataggag tagcggtgct cgtaggcgca
     5641 ttcgacgtcg ggcgcctcgg cgtcgaccag aacggcgtga cagccgctcc aatggccgac
     5701 tgcctgtgcg ccgaaactca gcgccaaact gagcacctcg ccggtgttgt agcgcgagcc
     5761 gcggactttc accaaatccc aaccggcccc aagatagcgc gcgcgcattt ccggattggc
     5821 ctcgaacccg ccggcagcca gcaccacggc gcggctctgg aaatccacca ggccctctgc
     5881 cgtttgcacc cgcacaccgg aaacccttcc cttgggatcg acgatcaaag cggtcgcctt
     5941 cgcctcgtaa ataatttcga tgccgttttt ctccgccgtc ggaaacagca tctcgaccag
     6001 gccaaggccg ccgtcgttca ccgatattac ccggcccgag ggaaacttga tcttgccgcc
     6061 catcttcact gcatgggtgc tggtggaaag tatccattta acgttcatgt cggtcagcca
     6121 acgcgccg
//
LOCUS       DASUTG010000264         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1227627, whole genome shotgun sequence.
ACCESSION   DASUTG010000264 DASUTG010000000
VERSION     DASUTG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227627"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(745..1020)
                     /locus_tag="VF089_06035"
     CDS             complement(745..1020)
                     /locus_tag="VF089_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173556.1"
                     /translation="MATALDRWDEEGGAQDVAWPLPYEASDLRDVERRVLECLGAALV
                     REWNDLPTDVQRRLFEHAASGKSHDATLLKTRIARFLHDHKGASGGR"
     gene            1230..1478
                     /locus_tag="VF089_06040"
     CDS             1230..1478
                     /locus_tag="VF089_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173557.1"
                     /translation="MDVIVTPAEGGTVWQLTDLLGRSMGRITASAPRQFMIHPEDHAS
                     ETMAGIQKGRHASLDAALAEIERHTRGVCRRNPGEDQS"
     gene            complement(1475..1804)
                     /locus_tag="VF089_06045"
     CDS             complement(1475..1804)
                     /locus_tag="VF089_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173558.1"
                     /translation="MTKRLKRVPSALSGLRQAEPPKDLGEHGNANDRAVTSAGAHIRL
                     APHAKIHKSFYRNRWPRHQAIWALEVGRDAWILSGKIKALPVKHIDLLAANIGCSADE
                     LGLLGAV"
     gene            1788..>2838
                     /locus_tag="VF089_06050"
     CDS             1788..>2838
                     /locus_tag="VF089_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX6173559.1"
                     /translation="MSRLVIVSNRVALPGEAAQRSGGLVTGLVDALHQAGGLWFGWSG
                     KLGETAAEPNVFETEGVTYATVDLTRADHDGYYSGFSNAALWPLFHYRLDLMNFTRRD
                     LAAYRRVNALLADALAPLLRPSDLVWVHDYHLIPMAEELRRAGAKQRIGFFLHTPFPS
                     LGVLATLPHYGDLLKSLCAYDLVGFQTVEDLTAFHDAIVRRAGGTVSSDGYVHAFDRV
                     LRAGAFPIGIDTEAIAEMASRAVKSRTARRLQESLRGRQLIIGVDRLDYSKGLEHRFK
                     AFAAFLDGYPAYRNHVRLLQIATATRSDVPEYRALRQRLGALAGDVAGRFAELDWAPI
                     QYLNRSFSQRSLAGFF"
BASE COUNT          559 a          866 c          824 g          589 t
ORIGIN      
        1 tgcactgatc atccgaggga cgagaatacg ttttgctcct tcaaacagga tatcgtgcgc
       61 tccagcgatt ggcagcgtac cgcaacggag acaaggcaat gatacgggat gagaagctcc
      121 aaaggatcat cgaacgcgct attcaatcag ctttcgttga ttttccggac gcgggaaacg
      181 ggacggtgtg gccgaaaact tacaaagagc ccaacgaatg caagacgatc gcaacagccg
      241 tactctccgc tctcaaaagt gcaggctaca aaatttcgct cgatgcaaac tgaggcatca
      301 cgggaagccg gcatgagcga tcttcgccaa ggtgcaaaga tccgccgcac atcctgctgc
      361 gcatcgaagc ggtatccaga tttggccggc cccttccggg aggctgcgtc taccgaccat
      421 ctccggcgat tgctctcgag ctgataattt acgaggcacg taagagcgtg tcagcgaggc
      481 tgacccaaca tctcgacaag cggtcgcgga ccgacggcga gttcatcagt ggcgatcaca
      541 cgattgattt ccaagtcata gtcgtcatac gacaagaccc ggcgtgccat gcgattttgg
      601 ggatatgcca gcgccagaga agcctcattt cttttgcgaa gatgcattga ggcgaattca
      661 accggatgcc cgcccctatg agaatactgg atcacttaaa tgtcgtttcg acgcgatcgg
      721 cgcaccttca gcgatcgcag gaacttagcg cccacccgaa gcgcccttgt gatcgtgcag
      781 gaaacgggcg atccgcgttt tcaacagggt ggcgtcgtgc gatttgcctg aggccgcgtg
      841 ctcgaaaagc ctgcgctgga cgtcagtagg cagatcattc cattctctga caagggcggc
      901 acccagacat tccaggactc tgcgctccac gtcgcgcaaa tcgctggctt catagggtaa
      961 aggccacgcc acgtcctgcg ctccgccttc ctcgtcccaa cggtcgagag cggttgccat
     1021 ttcttctccc gaagttggcc ttcgaccttt ctttttgatc attctgtatc gtcgcacata
     1081 ttggcaataa gcgccattcg ctcgatcgcg gattcgatcc gaagcgccaa tccttccagt
     1141 accgagaatg ccccggccac gaaactcggt agttcgcccg tcggttatgc aacgatttgc
     1201 tcgcgcggcg tacaggccca aggttcacca tggatgtgat cgttacaccg gcagagggag
     1261 gcacggtttg gcagctgacg gacctgctcg gccgctcgat ggggcgcatc acggcaagcg
     1321 ctccccgtca gttcatgatc catccggagg accacgcatc ggaaactatg gccggcatac
     1381 agaaagggcg gcacgcctcg ctcgacgccg cactggctga gatcgagagg cacacccggg
     1441 gcgtctgccg tcgaaatccc ggggaagatc agtcttagac ggcgcccaac aaccccaact
     1501 cgtcggcaga gcatcctata tttgctgcga gaagatctat atgttttaca ggtaacgcct
     1561 tgatcttgcc gctcaatatc catgcgtcgc gcccgacttc aagcgcccag attgcctgat
     1621 gacggggcca tcggttccgg tagaaggact tgtggatctt cgcgtgagga gcgaggcgaa
     1681 tgtgggctcc ggcggatgtc accgctcgat catttgcgtt gccgtgttcg ccgaggtctt
     1741 tcggcggctc ggcttgacgc agccctgaca gcgcggaggg gacccgcttg agccgcttgg
     1801 tcatcgtctc caaccgtgtc gccttgccgg gagaggctgc gcagcgcagc ggcgggcttg
     1861 tgaccggcct cgtcgacgct ttgcatcagg ctggtggctt gtggtttggg tggagtggca
     1921 agctaggaga aacggcagcg gagccaaacg ttttcgagac cgagggagta acgtacgcca
     1981 ctgtcgatct cacccgcgcg gaccatgacg gctactacag cggcttctcc aacgcggcgc
     2041 tgtggccctt attccactat cggcttgatc tcatgaactt tacccgccgg gacttggccg
     2101 cctatcggcg ggtgaatgcc ctgctcgccg acgccctcgc gcccctgctc aggccgagtg
     2161 acctcgtctg ggtgcatgac taccacctga tccccatggc cgaggaactt cgccgcgccg
     2221 gcgccaaaca acggatcggg ttcttcttgc atacgccgtt tccatctttg ggtgtgctgg
     2281 cgacccttcc ccactacggc gacctgctga aatcactgtg cgcctacgat ctcgtcggct
     2341 tccagacagt ggaggatttg accgccttcc atgacgccat cgttcgccgt gcaggaggca
     2401 cggtttcgtc cgatggctac gttcacgctt tcgatcgggt tctacgcgcc ggcgccttcc
     2461 cgattggtat cgatactgaa gccatcgccg aaatggccag tcgcgctgtg aaatcccgga
     2521 cggcacgtcg gctgcaggag agtctccggg ggcgccagct tatcattgga gtggaccggc
     2581 tggattacag taagggtctc gagcaccggt tcaaagcgtt cgcagccttt ctcgacggct
     2641 atcccgcgta ccgcaaccac gtccgcctcc tgcagatcgc caccgcaacg cgcagcgacg
     2701 ttcctgaata ccgtgcgctg cgacagaggc tcggtgcgct ggcgggcgac gtggccgggc
     2761 gcttcgcgga actcgattgg gcgccaatcc agtatttgaa caggagcttt tcccagcgct
     2821 cgctcgctgg cttctttc
//
LOCUS       DASUTG010000265         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1377698, whole genome shotgun sequence.
ACCESSION   DASUTG010000265 DASUTG010000000
VERSION     DASUTG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377698"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF089_06055"
     CDS             <1..349
                     /locus_tag="VF089_06055"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEX6173560.1"
                     /translation="DLIDRTCRLTLNGEVRTQNSPYLMRNLLLNPDARLRGWGFLKEH
                     WDEMLRQYPDNSIPRMCEGIIGLALPDLESEVREFFARHPVKQGVKQMEQHLERLHIS
                     VVCRERWQHQRLS"
     gene            454..2277
                     /locus_tag="VF089_06060"
     CDS             454..2277
                     /locus_tag="VF089_06060"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEX6173561.1"
                     /translation="MALTEDDAAKIVLIRSIEECDPAAFSEELRAQAFVAAQDAEPGL
                     SSIERYAARLFEHLSPWYQSILQLAKIPLPWTLPVCFAAFLLGLATNLLGPAQQIHVI
                     RNPIFILIAWNLLVFLALFALFLRRKIKTAQLSDVVRAQNNLPGAENVERLRSNLRNN
                     QSKASWVVRYLLPGVWLFFHRMIFAVHEQKNLAQIIRRFSHHWLSVAGTLVVARWRRL
                     LHLGSVFVASGAVAGMYFRGLFQGYRVVWDSTFIVDQESVNMFIRVLFGPSLWLSNVF
                     GLGLANEISVARLLTPGGDEADGWIHLFAISVLVAVVLPRLALAAWQSSRVGRLSKNI
                     ALPLDHYYGEVIEGPIRLLVENEVAAHAQTFSAKVAGFVASKLYDEQIVPKLISFREG
                     GGRIADLKSELTSTTVAFLPKIQSFITDTAIPELQLALSQRLGEVLKSIGTSFIDVKD
                     PHIVLEELSIFPAGRTELKISNEFSRAIALSVGTSIALAVAAVGGGIGEQLGIAIIAT
                     LLGTTGPIGFLIGLVAGAVVAAGAWWFGKDRITETVEHVLLPAAVVRMALWQSRFQRL
                     LAEAREKCEESVRAKVDENLQALIPQITNEILLRLRRLRQY"
     gene            2406..3539
                     /locus_tag="VF089_06065"
     CDS             2406..3539
                     /locus_tag="VF089_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173562.1"
                     /translation="MKDGFKVIDADRHILEPSDLYQKYLPEKFKNRVRLEGPNQTVRL
                     VDGKPVSDSAQRPGRVMEDFGYIFSSSKRWRECFADAYANKFDPASNVRDMDREGIDV
                     SVLFPTIGLYIMWRDDIDPELSAAICRAYNTWLAEYCDYDRKRLHGVMLIPLQDPARA
                     VEELKYAREKLGLVGIFWRPNKFCGRTLASPEYDPVYETAADLGVTVCVHEGARTVLP
                     QAGSDRYSEFGRHIACHPLEQMLACLNFCADGVLEKFPKLKVAHLESGCGWVPFWLER
                     MDEHWEHESHGSAKLTKEKPSYYFKRQCWVSCEAGEELAPTFVEHVGDDYLMIATDYP
                     HSDAIDKFPDKTVGAISSNEKISAESRRKILWDNPTKTFGLGA"
     gene            3709..>3876
                     /locus_tag="VF089_06070"
     CDS             3709..>3876
                     /locus_tag="VF089_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016541741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX6173563.1"
                     /translation="MLPYNRRLKTKARSLRTKPTDAELHLWHRLRRKQILGVQFYRQK
                     PIANYIADFYAP"
BASE COUNT          844 a         1025 c         1093 g          914 t
ORIGIN      
        1 ggatttgatc gatcgcacgt gccggctgac cctcaacggc gaagtgcgga cgcaaaactc
       61 cccgtacttg atgcgcaatc ttttactaaa tcccgacgcc cgcttgagag gctggggctt
      121 tttgaaagag cactgggacg aaatgttgcg ccaatatccc gacaactcga tcccgcgcat
      181 gtgcgaaggc attattggtt tggcactgcc ggatctcgaa agtgaagtga gagagttctt
      241 cgcacgacat ccggtaaagc aaggggtaaa acagatggag cagcatctcg agcggctgca
      301 catttccgtt gtctgccgcg agcgttggca gcatcaacgc ttaagttaat cagcccgttg
      361 atgccgcatg ctcgttcaat taattggaag cgcgcgctta aaggccggcg caaaggttcg
      421 ggctgttgag atgcaccccg agcttagacg accatggctt taaccgaaga cgacgctgcc
      481 aagatcgtat tgatccgttc tatcgaagag tgtgaccccg ccgcgttttc agaggaactt
      541 cgggctcaag catttgtcgc ggcacaggat gcggagcccg gattaagttc catcgagcga
      601 tatgcagcgc gcttgttcga acatctctcg ccttggtatc agtcaatcct gcagctggct
      661 aaaattccgc ttccctggac tttgcccgta tgctttgccg ctttcctctt aggtctggcg
      721 accaacctgc taggcccggc gcaacaaatc catgtcatcc gcaatccgat atttattcta
      781 attgcttgga acctgctggt gtttctggcg ctgttcgcgc tgttcttacg ccgtaaaatt
      841 aaaacggcgc aactgtcgga cgttgtccgc gcacaaaaca atttgcctgg cgccgagaac
      901 gtagagcgac tccgcagtaa tttacgaaat aatcagtcta aagcctcctg ggtcgttaga
      961 tacttgttgc ctggcgtgtg gctttttttt caccgaatga tctttgccgt ccatgagcag
     1021 aagaatctgg cccagatcat aagacgtttc agccatcact ggctctccgt agccggaacg
     1081 cttgtggttg cgcgatggcg gcgcttgctt catctcgggt ctgtgttcgt cgcctccgga
     1141 gccgtcgccg gaatgtattt tcgcggcctg tttcagggct atcgggtggt ttgggacagt
     1201 actttcatcg tcgatcagga atcggtcaac atgttcatcc gcgtgctgtt cggccctagc
     1261 ttatggcttt ccaacgtatt cggccttggg ctggccaatg agattagcgt tgcccggctt
     1321 ctcactcccg ggggagatga ggcggatggc tggattcatc tgttcgcgat ctcagttctg
     1381 gtcgccgtcg tcctgccgag acttgcgttg gcggcttggc agtcgagccg ggttggtcgt
     1441 ttatctaaaa atatcgcgct tcctctagat cactactacg gtgaagtcat tgaggggccg
     1501 attcggttgt tggtcgagaa cgaggtcgcc gcgcatgcac aaaccttctc cgccaaggtc
     1561 gccggctttg tcgcatcgaa gctttacgat gagcagattg ttcccaagct catctcattt
     1621 cgtgaaggag gcggaagaat tgctgatctg aagtctgaat tgacgagcac taccgtcgca
     1681 ttcttgccaa aaattcaatc cttcatcaca gatactgcga tacccgagtt gcagttggca
     1741 ttgtcacaac ggctgggtga ggtcttaaag agcatcggca caagctttat tgatgtcaaa
     1801 gatccgcaca tagtcttgga ggagttgtcg atatttcccg ccggtagaac tgaattgaag
     1861 atctccaatg aatttagccg cgcgatcgcg ttgtcggtag gcacatcgat tgcgcttgcc
     1921 gtcgccgcgg ttggcggcgg catcggagaa caactgggca tcgccatcat cgcgacgctt
     1981 ttgggcacca ccggccctat cggattcttg atcgggctgg tcgcaggcgc cgtggttgct
     2041 gctggagcgt ggtggtttgg caaggataga atcaccgaaa ccgtcgagca tgttctgctc
     2101 ccggccgctg tcgtgcggat ggcgttatgg cagtcgcgat tccagcgact gctggctgaa
     2161 gcgcgggaga agtgcgaaga atcggtgcgc gcgaaagttg acgagaacct gcaggcactg
     2221 attccgcaaa ttaccaatga aatcttgctg agattgcgcc gcctgaggca gtattaacgc
     2281 gccgtgtttt tcagcgatcc taaatgaatc ggccggccac ttgctgccgg gacaggattg
     2341 atttatgtag gcagtactga ctgagataaa taaaaacccc gagcagcgca gggaaggaga
     2401 cagttatgaa ggatggtttt aaagtcattg atgccgaccg tcacattctt gaaccgtcgg
     2461 atctatacca gaagtatttg cccgagaagt ttaagaaccg ggtgcgtctc gaaggaccta
     2521 atcagacggt gcgcttggtt gacggcaagc cggtgtccga ctcagcacag cggcccggcc
     2581 gggtcatgga ggatttcggc tatatttttt cgtcatcaaa acgctggcgc gaatgtttcg
     2641 ccgatgctta tgccaacaaa ttcgacccgg cttcgaacgt gcgcgatatg gaccgtgaag
     2701 gcatcgatgt cagcgtcctg tttcccacca tcgggctcta catcatgtgg cgcgacgaca
     2761 tcgatcccga actaagcgct gcgatctgcc gcgcttacaa tacctggttg gccgaatact
     2821 gcgactacga tcgcaaacgc ctgcatggtg tcatgttgat tccgctgcag gatccggcgc
     2881 gggcggtcga ggagttgaaa tacgcgcgcg aaaagttggg cctggtcggg atcttctggc
     2941 ggcccaacaa gttttgtggg cgaaccttgg ccagccccga gtacgatccg gtctatgaga
     3001 ccgccgccga tctcggcgtg actgtgtgcg tgcatgaagg cgcgcgcacg gtgttgccgc
     3061 aggccggctc cgatcgttat agcgagttcg gccgccatat cgcctgccat ccgctggagc
     3121 agatgcttgc gtgtctcaat ttctgtgcgg acggcgtgct ggagaaattt cccaagctca
     3181 aagtcgccca tctcgaatcc ggctgcggct gggtgccgtt ctggctcgag cgcatggatg
     3241 aacattggga gcacgaatcg cacggctcgg ccaagctgac caaggaaaaa ccgagttatt
     3301 atttcaaacg gcagtgctgg gtgagttgcg aggcgggcga agagctcgcg cccacattcg
     3361 ttgaacatgt cggcgacgat tacttgatga tcgccaccga ctatccgcat agcgacgcca
     3421 tcgataaatt cccggacaag accgtgggcg cgatcagcag caacgaaaaa atttccgccg
     3481 agtcgcggcg caaaatcctc tgggacaacc cgactaaaac tttcggtctc ggcgcatagc
     3541 atcgctcgat tagcgaaacg aaagcttcga gaaagaaaaa gcccggacga caaacccggg
     3601 ctttttttct tggctcttcg cataaacgag tgggtgaaat acgggtaaga aattctgctc
     3661 ttataggttt cacccccttt gaaaaagggg gattgggggg attttccgat gttgccatac
     3721 aatcgccgac ttaagaccaa agctcgctcc ttgcggacaa agccgaccga tgccgagcta
     3781 cacctgtggc accgcctgcg gcgaaaacaa atcctcggtg tgcagttcta taggcaaaaa
     3841 cccatcgcaa actacatcgc tgatttttat gcaccg
//
LOCUS       DASUTG010000266         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1822478, whole genome shotgun sequence.
ACCESSION   DASUTG010000266 DASUTG010000000
VERSION     DASUTG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822478"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF089_06075"
     CDS             complement(<1..210)
                     /locus_tag="VF089_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173564.1"
                     /translation="MTNVKGFIDADGHVVENDRALLDFLPQPYKGREDLLSFPFFPTL
                     DGFHRQALRVVDGKGRYVAQGSLKEW"
     gene            complement(417..1988)
                     /locus_tag="VF089_06080"
     CDS             complement(417..1988)
                     /locus_tag="VF089_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6173565.1"
                     /translation="MSLLRFFLEHRAEVTALIFEHLFLVGVSIGIALLIGVPLGILLT
                     RKPTLSNPVLGFANIMQTVPSLALFGFLIPVNLILFDIRILGGIGARTAIAALVLYAL
                     LPIIRNTFTGISGVDPAIREAGRGMGMTDRQLLLQVELPLALGVIIAGVRVATVICVG
                     TATIAAAIDAGGLGRYIFRGLRANDNVLILAGAIPAALIALIADLFLGLLERAIRFRS
                     AASRKVRKILWAALATALVLLVFATASFFRDYSASRIAVGSKDFTEQIILGELLAQAI
                     EGKTGLQVERRFGLGGNLAHHAMIAGEIDVYVEYTGTALLAILKNPPIKDPAEVYRQV
                     KSSYAKEFSSEWTEPLGFNNTFAILVRGDDARRHDLKTISDAARISSEWRAGFGQDFM
                     SRPDGYPGFARTYGFQFKEIREMDLSLTYRALAEKQVDLIAGNSTDGLISRYGLVQLE
                     DDLHYFPPYDAVPVVRHKALENHPELREVLEELGGILNVKEMRELNYAIDGEQRRAKD
                     VVREFLTRKRILNDR"
     gene            complement(1985..>2557)
                     /locus_tag="VF089_06085"
     CDS             complement(1985..>2557)
                     /locus_tag="VF089_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6173566.1"
                     /translation="RVEGTSTEAWDPIILRRRIGYVIQEIGLFPHLTIEENIGLVPRL
                     ESWPAEKIRNRARELLAMVSLEPDRFAPRYPAELSGGQRQRVGVARALAADPPMMLLD
                     EPFGALDPITRREIQQEFRSLQKQLGKTIVFVTHDVAEAFALATRIGLLKDGEMIVLG
                     LPAELLASNHPEARAFVQCFHDSEGAQSYS"
BASE COUNT          617 a          726 c          654 g          560 t
ORIGIN      
        1 ccattccttc agcgatccct gcgccacata acggcccttg ccgtccacca cgcgcaaggc
       61 ttgacggtga aatccatcca atgtcgggaa gaaggggaag gacaacagat cctcccttcc
      121 cttgtaaggc tgtggcagaa aatctagcag agcccgatca ttctccacca catgtccgtc
      181 agcatcgata aatcctttaa cgttggtcat cgaaactcct cacttccgtg gtttgtcgcg
      241 gacaaaagtt acacattcct tttttcgcta cggcttagcg tactagttac caagaaaagc
      301 tcaggctctg caacgcatga tttccgttca aactgtctgt gcgaaaatga gctgttgact
      361 gcaggtgttt tcgacagctc ctcggaagtt gccgcgagca agtcgtccga tcgatctcat
      421 cgatcattta agatcctttt gcgggtcaaa aattcacgaa ccacatcttt agctcgccgt
      481 tgttcgccat cgatggcata gttgagctca cgcatctcct tcacattgag aattccgccc
      541 aactcctcta atacctctct tagctccgga tgattctcca gagccttgtg ccgcacgact
      601 ggaacggcat cataaggggg aaaatagtga agatcgtcct cgagctggac caggccatat
      661 cggctgatca ggccatcggt cgaattcccc gcaatcagat ccacttgctt ctccgccaga
      721 gcgcgatagg tcaaagacag atccatttca cgtatttcct taaattgaaa accataggtc
      781 cgcgcaaagc cgggataacc gtcaggccgc gacatgaaat cctgaccaaa gccggcgcgc
      841 cattctgatg aaattctggc cgcgtcgcta atcgtcttta gatcgtgacg tcgtgcatca
      901 tcgcctcgaa ccaggattgc gaaggtattg ttgaagccca gcggttcggt ccattcgctg
      961 ctgaactctt tggcgtaaga cgatttgact tgacgataca cttcggccgg atccttgata
     1021 ggcggatttt ttaggatcgc caggagagca gtgccggtat attcgacata aacatcgatc
     1081 tccccagcga tcattgcgtg gtgagccaga tttccgccga gaccgaaacg gcgctccact
     1141 tgcaggccgg ttttgccctc gattgcctgg gccagcaatt cacccagaat gatttgctcg
     1201 gtgaaatctt tcgagccaac ggcgatgcgg cttgcgctgt aatcccggaa aaagctcgcg
     1261 gtggcaaata cgaggagcac cagcgcggtg gctagcgccg cccacaagat tttccgaacc
     1321 ttacgacttg ccgcgctgcg gaaacggata gctcgctcca agagtccaag aaacaaatcg
     1381 gcaatcaacg caatcaaagc agccggaatt gctcctgcga gaatcagcac gttatcattg
     1441 gctctcaacc cgcggaaaat gtatcgtccc aatccgccgg catcgatcgc ggctgcgatg
     1501 gttgcggtgc cgacacaaat aaccgttgcg acgcgtacac cagcgataat gacgccgagt
     1561 gcgagtggca gttcaacttg gaggagcagc tggcgatctg tcattcccat gccgcggccg
     1621 gcctcccgaa tcgccgggtc gacaccgctg attccggtaa aagtatttct gatgattggc
     1681 aacagtgcgt ataaaaccag cgcggcaatg gcagtgcggg cgccgatacc accgagtatg
     1741 cgtatatcga acaggatcaa attgaccgga atcagaaagc caaaaagcgc gaggctcggc
     1801 acggtttgca ttatgttggc aaaccccaga acggggttgc tcaaggtcgg tttgcgcgta
     1861 aggagaatgc cgagtggcac gccgatcagg agcgcaatgc cgatcgaaac tccgactaga
     1921 aacagatgct caaagatcaa tgcagtcact tcggcccggt gctcgaggaa aaagcgcagc
     1981 aagctcatga atagctctgc gccccttccg aatcgtgaaa acactggacg aaagctcggg
     2041 cctcaggatg attcgaagca agaagctcgg caggcaatcc cagcacgatc atctcgccgt
     2101 ctttcagtaa accgatgcgc gttgccaggg cgaaggcttc ggccacatcg tgagttacga
     2161 aaactatggt cttgcccaac tgtttttgca gcgagcgaaa ctcttgttgg atttcgcgtc
     2221 gcgtgatcgg gtccaaagcg ccgaaaggtt catctaaaag catcatcggc ggatctgccg
     2281 ccaacgcgcg tgcgactcca accctttggc gctgtccacc cgagagctcg gccggataac
     2341 gcggggcgaa tctgtcaggt tctaaactca ccatcgccag cagctctcga gcgcggtttc
     2401 tgatcttctc agccggccag ctttcaagcc gcggcaccaa cccgatattc tcttcaatcg
     2461 ttagatgagg aaataggccg atttcttgaa taacatagcc gatccgtcgc cgtagaatta
     2521 tggggtccca tgcttcggtt gaagttccct cgacccg
//
LOCUS       DASUTG010000267         4753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_932793, whole genome shotgun sequence.
ACCESSION   DASUTG010000267 DASUTG010000000
VERSION     DASUTG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932793"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VF089_06090"
     CDS             <1..569
                     /locus_tag="VF089_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_259446976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX6173567.1"
                     /translation="YLVTGRVEKLKEKYDLQIKFTQFPLHPETPVEGSPRGPEIIARN
                     RKMKVNMDREGLPFNPDRDMSYNSRLAQELAKWAASKGMEEKITDALFRAYFVDVKNI
                     GKADVLAQIAAENGLPGDEATDVLLSRAYKDAVDEDWRRCAAYGVNAVPTFLAGRYLM
                     VGAQPYEELERLIQHVMNSPQDQPPTNP"
     gene            651..1781
                     /locus_tag="VF089_06095"
     CDS             651..1781
                     /locus_tag="VF089_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173568.1"
                     /translation="MTYTMISADDHIDLGYLPRDLWTERVPKSLRERGPHVEDRGEKG
                     EYWVCDGETWAEYRGERWFAKTNRTRLALDRGGVGEAHRPTTPEKRLADMDRDGVEAS
                     LMFPPIIAMQVGDPELRNACVRAYNDWALEFGKTAPKRFFPVAMLSPVDARDAADEVV
                     RVAKLGFREANFLVNDVTLEMNLKPWDIFWAAAEDAGIVVSYHVGGSVQSGTVRATID
                     AVKPGERQGVFDMGLSNGATSFFNPFVNMFNFGTLERHPRLKFCLAESAIGWIPFVVQ
                     EMDYRYKRQFERRKSEDIPLKNMPSEIFKRQVWATYQTDLVGLHLIQFFGDGHIMWGS
                     DYPHPDSTWPFSKDFIEKDSAHLTPEMKRKVIRDNAAALYGV"
     gene            2092..3126
                     /locus_tag="VF089_06100"
     CDS             2092..3126
                     /locus_tag="VF089_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173569.1"
                     /translation="MASDEPKGAVHWIDHYVVGTNDLPAWTHWALNATGLPIRPINGL
                     TTNMRKKNTPIFCFMWWEGGSCRIGAFLQPEVYPPAKELGKDLPRCGFYIRPEDIDLH
                     LRRLDRHQIPHTDPVKTAAEGEEGTVIYFADPDGNQYEFWAPVNMPEGAMEIATSERV
                     GRISHAVYGSRDLARTAAFFEKYCGMQVERNAATSEGTLVLRLRAGARLVYKLVEQVD
                     ERIAGHSPWWDMHTALTVREQEFFPNYRRMWEGLPEEEGPKETLKDSRDKEDAFPART
                     GLHRSPVGYKWKEIYQRGDEFYDWDGHAFHFFGGIPLKDDGSLALYQGKEQEEYLREL
                     AESLKTRGEA"
     gene            3545..>4753
                     /locus_tag="VF089_06105"
     CDS             3545..>4753
                     /locus_tag="VF089_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6173570.1"
                     /translation="MKTETTQAKTRQSKDSSIDRPENLGTPQMKWGSDVVAEVTRRLN
                     LKYIALVPGASYRGFHDSIVNYLGNSNPQMVICLHEEHAVSIADGYGKVTEEPMAVAL
                     HANVGLMHAAMPIFNAWCDRTPMIIFGATGPVDAHRRRPWIDWIHTAKDQASIIRDYT
                     KWDDQPASPQAAVESVLRANQITRTPPYGPVYICLDAGLQEAPLTEELAVPDAARFTA
                     PPTPAVPKDSLHKALTAINQAKFPLILMGRVSRKQSDWDNRVRFAETIGAAVLTSSND
                     PASFPTTHPLHLAAPCLRPSKDATALIERADLIISLDWLDLAGVFRLSLGNAQTQVPT
                     DKTVIHVSLDSVRTNGWSMDHQALAAVDIPIFAEPDQFVAQMLEELDAKKTPKAKPRS
                     EMKEIPHWNAA"
BASE COUNT         1129 a         1326 c         1321 g          977 t
ORIGIN      
        1 gctacctcgt caccgggcgt gtcgagaaac ttaaagagaa gtatgacttg cagatcaagt
       61 tcactcagtt tccgcttcat cccgagactc ccgtggaagg tagcccacgc ggcccggaga
      121 tcattgcccg aaaccgcaag atgaaagtga acatggatcg cgaaggactg cctttcaatc
      181 ctgatcgtga catgtcgtac aacagccgtt tggctcagga gctggcgaaa tgggcggcaa
      241 gcaaaggcat ggaagaaaag attaccgatg ctctcttccg cgcctacttc gtcgacgtga
      301 agaatatcgg caaagccgac gttctcgctc agatcgcggc agaaaacggc ttgcccggag
      361 atgaagccac agacgtcttg ctaagccgcg cctacaaaga tgcggtcgat gaggactggc
      421 gccggtgcgc cgcctacggt gtcaatgccg tgccgacatt tcttgcgggc cgctatttga
      481 tggtgggcgc gcagccctat gaagaactgg agcggctgat tcaacacgtg atgaactcgc
      541 cgcaagatca accgcccacc aatccatagg ccatacgatc atgcataaac gtaaaaatat
      601 gacttgttaa cggggaagcg gccggctcgc tgccgattca aggagaagta atgacgtaca
      661 cgatgatttc agccgacgat catatcgatc taggctattt gccccgagac ctttggacag
      721 agcgcgtgcc taagtctttg cgcgagcgcg ggccgcacgt ggaggatcgg ggtgaaaagg
      781 gcgaatattg ggtttgcgat ggcgagacat gggccgagta tcgtggcgaa cgctggttcg
      841 ccaaaaccaa ccggaccagg ctggctctgg atcggggcgg agtcggcgag gcgcaccggc
      901 cgaccacgcc ggagaaacgg ctcgccgata tggatcgcga cggcgtcgaa gcttcgctga
      961 tgtttccacc aatcattgcc atgcaggtcg gcgatccgga gctgagaaat gcctgcgttc
     1021 gagcgtacaa cgattgggca ttggaattcg gtaagaccgc gcccaaacga tttttcccgg
     1081 tggcgatgct ctcccctgtc gatgctcggg acgccgcaga tgaagtggtc cgggtggcaa
     1141 agttaggatt tcgcgaagcc aatttcctgg ttaatgatgt gactctcgaa atgaacttga
     1201 agccatggga catattctgg gccgccgccg aggatgccgg cattgtggtc tcctatcacg
     1261 tcggcggcag cgtgcagtcc ggaacggttc gggcgaccat cgatgcggtg aaaccgggcg
     1321 agcgccaggg cgtgttcgac atgggcctga gcaacggcgc aacttcgttt ttcaatccat
     1381 tcgtaaatat gttcaatttc ggaactctgg aacgccatcc cagattgaaa ttctgccttg
     1441 ccgaatccgc tatcggctgg atcccattcg tcgtgcaaga aatggattac cgttacaagc
     1501 ggcaattcga aagaaggaag tccgaagaca taccgctgaa gaacatgccc agcgaaatat
     1561 tcaagcgtca ggtttgggca acttatcaga ccgatctagt cggactgcac ctgatccagt
     1621 tcttcggcga cggccacatc atgtggggct cggattatcc gcacccggac agcacctggc
     1681 ccttttccaa ggattttatc gagaaagact cggctcattt gacgccggag atgaagagga
     1741 aggtcatccg cgacaacgcc gcggcgctct acggagtcta aagaaaatag ccgttcaaca
     1801 gttcaaatgt tcaacgttca aggatcgaac tcagaccggt ggagcacccc cacgtttttg
     1861 atactttgtt gcccttgatc aatttgcggc atgtgagagt cataggattc aaacatagga
     1921 attactggag cttcgtaact tcgtggcagc gtccggctat ctgaccttga accgttgaac
     1981 ggttgaacta ttgaactgtt aaacgtcttg cttgaggacc gcttgacacc ttttccccag
     2041 tctagtaccg ctcggattga aacaccttct cttgtgtgaa cggaggattt tatggctagc
     2101 gatgaaccga aaggcgcagt tcattggatc gatcactacg tcgtcggcac caacgattta
     2161 ccggcgtgga ctcactgggc gcttaatgcc acgggccttc ccatcaggcc aatcaacggc
     2221 ctgaccacca acatgagaaa gaagaacacc ccgatctttt gctttatgtg gtgggagggc
     2281 ggatcgtgcc gcatcggcgc gttccttcag cctgaggttt acccgccggc aaaagaactg
     2341 ggcaaggatc ttccccggtg cggcttctac atccgtcccg aagacatcga cctgcacctg
     2401 cgccggcttg atcggcacca gatccctcat accgatccgg tcaaaacagc ggccgagggc
     2461 gaggaaggca cggtcatcta tttcgccgat ccggatggca atcagtatga gttctgggct
     2521 cctgtgaata tgccggaagg cgcgatggag atcgccactt cagaaagagt cggccgtatc
     2581 agccacgcgg tttacggttc acgtgatctg gcgagaaccg ccgccttctt tgaaaaatac
     2641 tgcggcatgc aagtggagcg taatgctgcg acttccgagg gaacgctggt gttacgtctc
     2701 cgggctggcg ctcggctggt ttacaagttg gtggagcagg tcgatgagcg gatcgccgga
     2761 cacagcccat ggtgggacat gcataccgcg ctgacggttc gcgaacagga gttctttccc
     2821 aactaccggc gcatgtggga aggactaccc gaggaagaag ggccgaagga gacgctcaaa
     2881 gattcccgcg acaaagaaga cgcctttcct gcccgaacgg gcttacaccg cagcccggtg
     2941 ggttacaaat ggaaggagat ttatcaacgc ggcgatgagt tctacgattg ggatggccac
     3001 gcgtttcact tttttggcgg cataccgctg aaagacgacg gctcgctcgc attatatcag
     3061 ggcaaagaac aggaagaata tctacgcgag ctggctgagt ctctcaagac acgcggcgaa
     3121 gcgtaatgat tttaaatctc ggtcgctcac ctacccgaac gagagattta tcattacgaa
     3181 ggctacgaag tatgtcatga gcttgcactc gcccacagag attcaaaatg gaatacgtcc
     3241 ttcaagccgc taattttcgg cgagtctgtc actcccgcat gtttctagcg ggaatccagg
     3301 ccgaagtcgg actggacccc cgattaaaac attcgggggt gacgattttg gggaatcgca
     3361 tctcttcaat tagcggcaat tttcgaagga ggccgcgaag gtcacgaagt taagatcaat
     3421 gttctgaacc cttcgtgccc atcgtgttct tcgcggtgag aaattgttac gagaccacca
     3481 actaacttct cattttcaaa taatcccaag gggactcgct taaccccaca aggagacaaa
     3541 tccagtgaag acagaaacga cgcaggcaaa aactcggcaa tccaaggaca gcagcatcga
     3601 ccggcccgag aatctcggca cgccgcagat gaagtggggc agtgacgtcg tcgctgaggt
     3661 cacgcgcagg ctgaatctga aatatattgc gctggtcccc ggcgccagct atcgcggatt
     3721 tcacgacagc atcgtgaact acctcggcaa cagcaacccg cagatggtca tttgcctgca
     3781 tgaagagcat gccgtttcga tcgccgatgg ctacggcaaa gtcactgagg agccgatggc
     3841 tgtggcactg cacgccaacg tcgggctcat gcacgcggcg atgccgatct tcaacgcctg
     3901 gtgcgaccgc acgccgatga tcatcttcgg cgccaccggt ccggtggacg cccatcgacg
     3961 acggccctgg atcgactgga ttcacacggc caaggatcaa gcctccatca tccgcgatta
     4021 cacaaaatgg gatgatcaac cggcatcacc tcaggcagca gtggaatcgg tgctgagggc
     4081 caaccagata acccgcactc cgccctatgg acccgtctat atctgtctcg acgccgggct
     4141 tcaggaggcc ccccttacgg aggagcttgc agtccccgat gcggcgcggt tcaccgcccc
     4201 accgaccccc gcggtcccta aagactcact gcacaaggcg ttgaccgcaa tcaaccaggc
     4261 aaagtttccg ctcattctca tgggccgggt ttcacgcaag caatccgact gggataaccg
     4321 cgtgcgcttt gccgagacca ttggcgccgc ggtactgacg agcagcaacg accccgcgtc
     4381 ttttccgacg actcatccgc tgcatttggc cgcgccctgc ctgcgcccaa gcaaagacgc
     4441 tacagctctg atcgagcgag cagacctgat tatcagtttg gattggctcg atctcgccgg
     4501 agtatttcgt cttagccttg gcaatgcgca aacccaggtt cctacggata agaccgtcat
     4561 ccatgtttct ctcgacagcg ttcgcaccaa cggctggagc atggatcatc aagccctggc
     4621 cgctgtcgat ataccaattt tcgcagagcc cgatcagttt gttgcgcaga tgctcgaaga
     4681 gctcgatgcc aaaaaaactc ccaaagccaa accacgatcc gagatgaaag agatcccgca
     4741 ctggaacgct gca
//
LOCUS       DASUTG010000268         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_486865, whole genome shotgun sequence.
ACCESSION   DASUTG010000268 DASUTG010000000
VERSION     DASUTG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486865"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF089_06110"
     CDS             complement(<1..311)
                     /locus_tag="VF089_06110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HEX6173571.1"
                     /translation="MSEIIFKPIGQIKAQFSDADVRAERKDLEGELEVYPQFEPALEG
                     IDGFSHLFLLAYFDRLRPDQIGPLQVKPRRLVNRGFKLGELPSLGVFALDSPTRPNPI
                     GL"
     gene            complement(374..1489)
                     /locus_tag="VF089_06115"
     CDS             complement(374..1489)
                     /locus_tag="VF089_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6173572.1"
                     /translation="METKKIETFDDWKDIFHMWQKDINYDTALFDSVLQGYEFTERFA
                     ESKHMEVEFGEFTGASKWRDPSEISRPEVKDLLLRLIAIQGDTEFASVELQRKLLDSA
                     PSERDLRSIVRINAEEMRHGWQMSYLLVRYFGADGKAEARKLLERRADKKERLLGAFN
                     EPVEDWIDFFAFTAFVDRDGMYQLKMLSHSGFAPLSGSMGPMLNEESFHLITGLSGLQ
                     RILRAGKVPVAILQKVLNRWLPVCFDLFGHERSRGAGNAYQWGLKGRFNEEDAGPVKD
                     PQSVNQGARELYYKEVQGLVDLLNKDVGAEQPKLYVPDIKFRRGIGEYRDKRHSVTGE
                     ALDEEAYTRHLQETLPTQKDREKLRKITREADWVAAN"
     gene            complement(1670..3277)
                     /locus_tag="VF089_06120"
     CDS             complement(1670..3277)
                     /locus_tag="VF089_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX6173573.1"
                     /translation="MIGRREFIKAGVVAGAGIMAGCVQSRRLLQLDRGEKDEGGSLGK
                     FVEPLIIPAALRPSLGEPLEVAMTETRQVLHRDLGESSVWAYSGGYPGATIEAQRNQP
                     LRVRWINRLPARHRLHVDHCLHGPHAYPDKLEHPRPRTVVHLHGGHVPPESDGYPEAT
                     LLPGESAIYEYPNTQNGATLWYHDHALGVTRLNVYMGLAGFYLIRDESEFALNLPRGQ
                     FEVPLVIQDRSFAADGSLDYPKSWEEEFFGAAILVNGRVWPYLDVRPRKYRFRILNGS
                     NSRTYTLALDSGQSFYQIGSDGGFLPEPVEVRQITLTAGERADVIVDFSKRTGELHLV
                     NSAPAPFPGKAGEGVIREVMQFRVRGTRGDDSALPSRLAAVPRLRESAAVKFRQFNLD
                     MVDESPKCTEPGFRWLINGLGWDDITEYPELGSTEVWSFSNLSPDVHPIHLHLVQFQI
                     LDRQKLQSDPARPGFFLPLTVPATRPEPPEPNEAGWKDTFRSMPGEVTRIIAVFADFA
                     GKYPYHCHILEHEDHEMMRQFEVVKLK"
     gene            complement(3530..4234)
                     /gene="gpmA"
                     /locus_tag="VF089_06125"
     CDS             complement(3530..4234)
                     /gene="gpmA"
                     /locus_tag="VF089_06125"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX6173574.1"
                     /translation="MKTLVLLRHGESTWNNENRFTGWVDVGLTDRGVQEAVKAGETLR
                     KKGYTFDLAYTSVLKRAIKTLWMVLEEMDLMWIPVHRSWRLNERHYGALQGLNKAETA
                     ARFGIEQVRIWRRSYDIPPPALSKNDERYPAKDPRYMSLKAEEIPLTESLENVTARLL
                     PYWHGSIAPAIRDGKRVLIVAHGNSLRALLKFLDGISDAEIVEVNIPTGIPLVYELRE
                     DLTPLRSFYLSDSVSV"
BASE COUNT          971 a         1319 c         1181 g         1012 t
ORIGIN      
        1 agtccgatcg ggttaggccg agtcggggaa tccagcgcga aaacgcccag cgagggaagc
       61 tcgcccaatt tgaaacctct attgaccagc cgccttggct tcacttgcaa aggaccgatc
      121 tgatcgggtc tgagcctgtc aaagtacgcc aaaagaaaaa gatgagaaaa accgtcgatc
      181 ccctccagag ccggctcgaa ctggggataa acttccagct ccccctcgag atctttgcgt
      241 tcggcgcgaa catcggcgtc ggagaactgt gctttgatct ggccgatcgg cttaaaaata
      301 atttcagaca taaataaata tctccactat atatctccgc tccgctggcc gcggtttcga
      361 acggtaacac tccttagttt gccgcgaccc agtccgcctc tcgcgtaatc ttccgcaact
      421 tttcccggtc tttttgcgtc ggcagtgtct cttgaaggtg gcgcgtataa gcctcctcgt
      481 cgagagcttc gccggtcacg ctgtggcgtt tgtccctgta ttcgccgatg ccgcgccgga
      541 acttgatgtc cggcacgtaa agtttcggct gttctgcgcc gacgtcttta ttgagaaggt
      601 cgaccagccc ctgaacttct ttatagtaaa gctcgcgtgc cccctggttg acgctttgcg
      661 gatcttttac cggtccggca tcctcctcgt tgaaccgccc cttcagtccc cattgataag
      721 cattgcccgc tcctcgcgag cgctcgtgcc cgaagagatc aaagcagacg ggaagccaac
      781 ggttcaagac cttctgcaaa atcgccaccg gaacttttcc tgcccgcagg attctctgca
      841 gacccgaaag tccggttatc agatgaaatg attcttcatt caacatcggt cccatgctgc
      901 cgcttagggg tgcaaacccc gaatggctga gcatcttgag ctgatacatg ccgtcgcgat
      961 cgacaaacgc ggtaaacgca aaaaaatcga tccagtcctc gaccggctca ttgaacgccc
     1021 caagcagccg ttccttcttg tcggcgcgtc gctccagcag cttgcgtgct tcggccttgc
     1081 cgtcggcacc aaaatagcgc accagcaggt agctcatctg ccagccgtgg cgcatctctt
     1141 cggcattaat cctgacgatg ctgcgcaggt ccctttcact gggagccgaa tccaggagct
     1201 tgcgctgtaa ttccaccgag gcaaactcgg tgtctccctg aatggcaatg agcctgagga
     1261 gcaggtcttt gacttccggt cgcgatattt cggagggatc gcgccacttc gacgcgccgg
     1321 tgaactcccc gaactcgact tccatgtgct tcgactcggc aaacctctcg gtgaattcat
     1381 agccctgtag aacggaatca aagagcgcgg tgtcgtagtt gatgtctttc tgccacatgt
     1441 gaaagatatc tttccaatcg tcgaatgttt cgattttttt ggtttccatg ccgacctcca
     1501 actatgaaga actgcaactc acgtgacagc gatcgggcga tcgcatgaga ctcgaaaatc
     1561 cgcagtgcaa ttggtaagag acacggcggt gttttatcac ttgaaccaga aaccttgaac
     1621 cggggccgga acttggcagt ctaatgagcc tttctcacca gccggcatct catttcagct
     1681 tgaccacctc gaactgacgc atcatctcat ggtcctcgtg ctcgagaata tggcaatggt
     1741 acggatattt gccggcaaaa tccgcgaaaa cggcgatgat cctcgtaact tctccaggca
     1801 tcgaccggaa agtgtctttc caccccgctt cgttgggctc gggcggctcg ggccgcgttg
     1861 ctggcacagt cagcggcagg aagaaccccg ggcgcgccgg atcggattgc agtttttgcc
     1921 gatcgaggat ttgaaactga accagatgga gatggatcgg gtgaacatcc ggtgagagat
     1981 tggaaaagct ccacacctcg gtgctcccca actcgggata ttcggtaata tcgtcccaac
     2041 cgaggccgtt gatgagccag cggaatcccg gctcggtgca cttgggactc tcatcgacca
     2101 tatcgagatt gaactgccgg aatttgaccg ccgccgattc gcgcaagcgc ggcactgctg
     2161 caagcctact cggcagagcg ctatcgtcac cgcgcgtgcc ccgcacgcga aactgcatga
     2221 cttcacgaat gacgccttct cctgcttttc cgggaaaagg cgccggcgcg ctgttcacca
     2281 gatggagctc gcccgttctt ttggagaaat ccacaatcac atccgcgcgc tcgccggccg
     2341 tcaaggtgat ttgtctgacc tcgaccggtt ccggcaagaa tcctccatcg ctgccgatct
     2401 ggtaaaaaga ctgtcccgaa tcgagagcca gcgtgtaggt gcgggagttg gagccattga
     2461 gaatgcgaaa acggtatttc ctgggtctca cgtccagata cggccatacg cggccattca
     2521 ccaggatggc cgcaccgaag aactcctcct cccagctttt cggataatca agagagccgt
     2581 cggcggcgaa tgatcgatct tgaatcacca gcggaacctc gaactggccg cgcggcaagt
     2641 tgagcgcgaa ctccgactcg tcgcggatca aatagaaacc ggcgaggccc atatagacgt
     2701 tgagcctcgt gacacccaag gcatgatcat ggtaccaaag agtggctccg ttctgagtgt
     2761 tcgggtattc gtaaatcgcc gactctcccg gcaacagggt tgcttcggga taaccatcgc
     2821 tttccggcgg cacatggcca ccgtgcagat gaaccaccgt acggggtcgg ggatgctcta
     2881 gcttgtcggg gtatgcgtgc gggccgtgca agcaatgatc aacgtggagc cggtggcgag
     2941 ccggaaggcg attgatccaa cgcaccctta ggggctggtt ccgctgagcc tcgatagtgg
     3001 ctccaggata accgccgctg taagcccaga ccgagctttc gccgaggtct cgatggagta
     3061 cctggcgtgt ttccgtcatg gcgacttcca acggttcgcc caaagacggc cgcaacgctg
     3121 cagggatgat aagtggttcg acaaattttc cgagactgcc gccttcatcc ttctcgcccc
     3181 tgtcgagctg caacagccgg cggctctgca cgcacccggc cattatcccc gctcccgcaa
     3241 ccacccccgc tttgatgaat tctcgcctgc caatcatttt gacctaccga gttcggcaca
     3301 ggatacggtc agtttgccac aaagagcctg cgcgatcata gccgccctgc aaaagattca
     3361 cgatgaattg cagccttcga gtaaacgagt aaatagtcga gcgcagtacc gcgcatctca
     3421 gacaccataa atcaattcca tcgggcccct agacgaggga agcttacgag gttcgctggc
     3481 tatttgggcc ggcggcatat tggtccggac acttactgaa tgacaattcc taaacactca
     3541 ctgaatcaga aagataaaag cttcggagtg gcgtcagatc ttctctgagt tcataaacca
     3601 atgggatacc ggttggaatg ttcacctcaa cgatctctgc atcggagatc ccgtcgagga
     3661 atttgagcaa agctctaaga ctattcccgt gcgcaacaat caagactcgc tttccatcgc
     3721 ggatcgctgg cgcgatgctc ccgtgccagt aggggagtaa gcgagccgtc acgttctcaa
     3781 gactttccgt cagcggaatt tcctcggctt tgagtgacat gtaccgcggg tccttggccg
     3841 ggtatcgttc atcattcttc gacagtgcgg gaggcggaat gtcatagctc ctccgccaaa
     3901 ttctgacctg ttcgattcca aaccgcgccg ctgtctctgc cttgttgagt ccctgaaggg
     3961 caccatagtg gcgctcgttc aggcgccagc tccgatgaac gggaatccac atgaggtcca
     4021 tttcttctaa taccatccaa agtgttttga ttgcccgctt gagaaccgaa gtgtaggcga
     4081 ggtcgaatgt gtagcccttt ttccgcaatg tttctccggc cttcactgcc tcctgcactc
     4141 ctctatcggt taggcccaca tcgacccagc ctgtgaagcg attttcgttg ttccaggtgc
     4201 tttcgccatg gcgtagcaga acgagggttt tcacagatcc tctcctgata tgcgggtaag
     4261 cgtcgaccgg ctgtaaagga cggtactccg cagcgaggct gtcgattctc tgaccagccg
     4321 ctggcttcca ctctggaatc cccggcaaca tgatcgggcc tgttcaagga cagaaccggt
     4381 tgcccatcag ttcattagaa cctcttgatg ctaaactgct ccaagaaaat cggttgagac
     4441 ggtccaagcc gtcccaacca gctgttgttt gtacccgcgg gtg
//
LOCUS       DASUTG010000269         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_932983, whole genome shotgun sequence.
ACCESSION   DASUTG010000269 DASUTG010000000
VERSION     DASUTG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932983"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF089_06130"
     CDS             complement(<1..355)
                     /locus_tag="VF089_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX6173575.1"
                     /translation="MDVQELSYDLPESLIAAFPSGNRQDSRLLVIRRTSGELIHTRFS
                     ELGNFLDPGDLLVLNDTQVFPARLHGTKESGGKVEVLLLQRFPDKRSLWIAVINAAKK
                     PKIGSRLLFGGMSAEV"
     gene            complement(432..1193)
                     /locus_tag="VF089_06135"
     CDS             complement(432..1193)
                     /locus_tag="VF089_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX6173576.1"
                     /translation="MFGIGAPITFLIIIIAAIISGVKVLKEYERGVVFRLGRMVGARG
                     PGLVYIIPGIEKMVKLDLRTVTMDIPPQDVITRDNVSVKVNAVLYFRVLDPNLAVREV
                     ENYLFATSQLAQVTLRSVCGQGELDELLAEREKINTRIQDILDKQTDPWGIKVVLVEL
                     KHIDLPQEMQRAMAKQAEAERERRAKVIHAEGEFQASQKLLDAAEVMSKQPMALQLRF
                     LQSLVEIASEKNSTTIFPVPIDLLAPFLTKSNQTS"
     gene            complement(1212..2525)
                     /locus_tag="VF089_06140"
     CDS             complement(1212..2525)
                     /locus_tag="VF089_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX6173577.1"
                     /translation="MKTLKIFFLVSLMAFGMSVLAQTNDSATPHVDLIVIDGSINPAV
                     DDFIRESIARAKSNGARALIIQLDTPGGLLSSTRTIVKDLLSAEVPIMVYVAPSGAGA
                     GSAGVFITMAAHIAAMAPATNIGAAHPVAGGGQEVKGVMGEKIENFTASFSETIAQKR
                     GRNTEWAIEAVRKSVSITETDALKKNVIDVIAKNLDDLLRQADGRKVDLDGRMTTLSL
                     KQVNVVRHEMSLKQRIVNAIADPNIAYMLLMAGILGLYMEFSNPGVLFPGITGAICLV
                     MALISLQLLSFNYAGLFLIILGIALLVGEAFMPSFGILGIGGVISLALGSFLLFDTPN
                     SDLTIDGTIVYTAVATMASLVLAIGYLVFRSQKARPTLGMDGLIGEIGEVRATLSPSG
                     KVFVHGEYWNAQTQTDGQIDVGDKVQIVGYEGMNLKVKRLQDGAS"
     gene            complement(2512..3285)
                     /locus_tag="VF089_06145"
     CDS             complement(2512..3285)
                     /locus_tag="VF089_06145"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6173578.1"
                     /translation="MKDLVSISTSEGDELATLVVSLRRLLQRQQRLGIRFVARPEVGT
                     MTPAAMGSNKPAADAPQFMSTQPDKGNVTSLQELRNHIGDCRRCKLHSARTHVVFGIG
                     NPTARLMFVGEGPGRDEDLKGEPFVGRAGQLLTDIITKGMGLRREDVYIANVVKCRPP
                     QNRNPEPDEVASCEPFLKKQIELIRPEIIVALGKFAVQTLLQSKVPITRLRGNWHSYM
                     GIKLMPTFHPAYLLRNPADKKLVWEDIKKVMKEMESENA"
     gene            complement(3397..>4399)
                     /gene="coaBC"
                     /locus_tag="VF089_06150"
     CDS             complement(3397..>4399)
                     /gene="coaBC"
                     /locus_tag="VF089_06150"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX6173579.1"
                     /translation="FNLTQESEIGHINLADSADIFVVAPATANVIGKIAAGIADDLLT
                     TVLMATQAPVLIAPAMNIHMYENPILQENLRKLRRIGYEILEPGEGYLACGYEGKGRL
                     PDPETILEQIRRQLKNKDLTGEKLVVTAGPSREPLDPVRYISNRSSGKMGYALARAAA
                     RRGAEVVLISGPTALTPPPGVKLRAVNTASEMRQSILDEYATCTGVIMAAAVADYHPA
                     QFSADKIKRGTGPLEVRLEPNPDILKELGQRKDGKLLIGFAAETGNLEANAEKKLREK
                     NLDMIVANDVTQEGGGFDGDTNIATIIDRRGATRSLPLMSKDELADQVFDHLLTFKR"
BASE COUNT          973 a         1220 c         1137 g         1069 t
ORIGIN      
        1 tcacctcggc gctcatgccg ccgaataaca accggcttcc tattttgggc tttttggcgg
       61 cattaattac cgcaatccaa agactgcgtt tatccgggaa gcgctggagc aagagtactt
      121 cgaccttgcc gccgctttcc ttggtgccat gcaaccgtgc ggggaatacc tgagtatcgt
      181 tgagaaccag caagtcgccg ggatcaagaa aattgcccaa ctctgaaaaa cgtgtgtgga
      241 taagctcgcc gcttgttcgg cggatgacga gcaggcgaga gtcttgccga ttgccgctgg
      301 gaaatgccgc aataagggac tcggggagat cgtaggataa ctcttgaaca tccatctcgt
      361 tcgggaggca gatctctacc tgtcgacagc catttcaatg ccgcaaatca tacatggcgg
      421 cgccgattaa atcatgacgt ctgattggat ttcgtgagaa acggcgccaa cagatcgatg
      481 ggtaccggga agatggttgt ggaatttttt tccgaggcta tctcgaccag gctctgtaga
      541 aagcggagct gtaaggccat gggctgtttc gacatcactt cggctgcatc caagagtttt
      601 tgcgatgctt gaaattcacc ctcggcgtga atcactttgg cgcgtctttc cctttccgct
      661 tccgcttgct tggccatcgc tcgctgcatc tcctgaggaa gatcaatgtg cttgagctca
      721 actagcacga cttttattcc ccagggatcg gtctgcttgt ccaagatgtc ctgaatgcga
      781 gtgttaatct tctcgcgttc agccagaagc tcgtccagct ctccttgacc gcaaacactg
      841 cgcagggtga cttgggcaag ttgggaggtt gcgaagagat aattctccac ttctctaacc
      901 gctagattcg gatccaacac cctgaagtaa agcaccgcat tcactttcac ggagacgttg
      961 tctcgggtaa tcacatcctg gggtggaata tccatggtca ccgtgcgcag atccagcttg
     1021 accatctttt cgatgccggg aatgatatag accaaccctg gcccgcgcgc gccgaccatt
     1081 cggccgagcc ggaacactac tccgcgttcg tactccttga ggaccttcac gccactgatg
     1141 atcgcggcga tgatgatgat tagaaacgtg atcggggcgc ctattccgaa cataattcct
     1201 cctccgctaa gtcacgaagc tccgtcctga agccgcttca ccttgagatt cattccctcg
     1261 tagccgacga tttgaacctt gtcgccgaca tcgatctgtc cgtctgtttg tgtttgagca
     1321 ttccagtatt caccatggac gaacactttg cccgacggac ttagtgttgc tctgacctcg
     1381 ccgatctcgc caattaatcc gtccataccg agcgtcggtc tcgccttttg ggaacgaaaa
     1441 accagataac cgatcgcaag aacaaggctc gccattgtcg ctacggccgt ataaacaatc
     1501 gttccatcga tggtcaggtc cgagttcggc gtatcgaaaa gcaaaaagga gcctaacgca
     1561 agagagataa ccccgccgat cccaagaata ccgaaacttg gcatgaacgc ttcaccgacc
     1621 aaaagcgcaa tgccaagaat gatcaaaaac aaaccggcat aattgaacga gagaagctgc
     1681 agtgagatca acgccatcac caggcagatg gcgccggtta ttcccggaaa taaaacgccg
     1741 ggattggaga actccatgta gagcccgaga atgcccgcca tcagcagcat gtaggcgatg
     1801 ttgggatcag caatggcgtt gacgatcctt tgtttaagac tcatctcgtg ccggacgaca
     1861 ttaacctgtt tgagggaaag cgtcgtcatg cgcccatcaa ggtcaacttt acgaccgtcg
     1921 gcttgcctca gtaaatcatc taggtttttg gcaatgacat cgatgacgtt tttcttgagc
     1981 gcgtcggtct cggtaatgga aacgctcttg cgcactgcct cgattgccca ctcggtattg
     2041 cggccgcgct tttgcgcaat ggtctcgcta aaggaggcgg taaagttttc gattttttca
     2101 cccatcactc ctttcacctc ctgtcctccg cccgctacag ggtgagccgc gccgatgttc
     2161 gtggctgggg ccatcgccgc gatatgcgcc gccatggtga tgaaaacccc ggccgatccc
     2221 gcacccgcgc cactgggcgc tacatagacc atgatcggaa cctctgcgct aagcagatct
     2281 ttcacgattg tccgggtgga gctcaaaagc cccccgggcg tatcgagctg aataatcagc
     2341 gcccgggcac cattggattt cgctctggcg atgctctcgc ggatgaaatc gtccactgcc
     2401 ggattgatcg aaccatcgat gacgatcaga tcgacgtgcg gcgttgccga gtcgtttgtc
     2461 tgtgccagca cagacatccc gaaggccata agtgacacca agaaaaagat cttaagcgtt
     2521 ttcactttcc atctctttca tcaccttttt gatgtcttcc cagactagtt tcttgtcggc
     2581 cggattgcgt aacaggtagg cggggtggaa tgttggcatc aacttgattc ccatatagct
     2641 gtgccaattg ccgcgcaacc gggtaatcgg aactttactt tggagcagtg tttgtacggc
     2701 aaatttcccc aacgcgacaa tgatctcggg ccgtatcaat tcgatttgct tttttagaaa
     2761 aggctcgcaa gaggcaactt cgtcgggttc tgggttgcga ttttgcggcg ggcggcattt
     2821 gactacattg gcgatgtaaa cgtcttctcg cctcaatccc atgcccttgg tgataatgtc
     2881 agtaagcagc tgcccggcgc gcccgacaaa aggctcgccc ttgagatctt cgtcccgtcc
     2941 cggaccctcg ccgacaaaca tcagcctggc agttggatta ccaattccaa ataccacatg
     3001 ggttcgagcg gaatgtaatt tacagcgccg acagtcaccg atatggttgc gaagttcttg
     3061 taggctggtc acgttccctt tatcgggttg agtcgacatg aactgcggcg catcagccgc
     3121 cggcttgtta ctccccatcg ctgctggcgt catcgtgcca acctcgggtc gcgccacgaa
     3181 ccgaataccc aggcgttgtt gtctttgtaa aagccggcgc agggaaacaa ccaaagttgc
     3241 cagttcgtcg ccctcactgg tggatatgga gactaaatcc ttcacgcgaa gatcttaaca
     3301 ccggagactt gcggctatca atgacagaat ccgcgccgta ggagcaactg gcattgattc
     3361 gatctgctga taatcacgat taaaatgcgc tctaggttaa cgcttaaagg tgagaagatg
     3421 atcaaaaacc tggtccgcca attcatcctt actcatgagc ggcagcgagc gggttgcgcc
     3481 ccgacgatct ataatggtcg cgatgttggt atcgccgtcg aaaccgccgc cctcctgggt
     3541 gacgtcattt gcaacgatca tgtcgaggtt cttttcacgc agctttttct cggcgttggc
     3601 ctccaggttg ccggtttcgg cggcaaaccc gattaatagt tttccatcct tgcgttggcc
     3661 gagctccttg agaatatccg ggttcggctc gaggcgcact tccagcggtc ccgtgcctcg
     3721 ttttatcttg tcggctgaaa attgggccgg atgataatcc gcaaccgcgg ccgccatgat
     3781 tacgccagtg catgtggcgt actcgtcgag tatcgactgg cgcatttcag acgctgtgtt
     3841 gacagccctt agcttaacac cgggaggcgg cgtgagcgcc gttgggccac tgatcagcac
     3901 gacttctgct ccccttcgcg ctgccgctcg cgctagagca taacccattt tccctgatga
     3961 tcggttcgag atatagcgaa ccgggtctag tggttcccgg ctaggaccgg cggtaacgac
     4021 cagcttttca cctgtcaaat ccttattttt aagctggcgc cggatctgtt cgagaatcgt
     4081 ctcgggatcc ggcaatcgtc ccttgccttc gtacccgcac gccagatagc cctcgcccgg
     4141 ctccagaatt tcatagccaa tccggcgtaa cttgcgtagg ttttcttgca gtatagggtt
     4201 ttcgtacatg tgaatgttca tcgcaggcgc aatcagcact ggcgcctgag tagccataag
     4261 cacggtggtc aatagatcgt cagcgatccc agcggcaatt ttaccgataa cattggcggt
     4321 agcgggcgca acaacaaaga tatcggctga gtcggctaga ttgatgtgcc ctatttctga
     4381 ctcttgcgtc agattgaaa
//
LOCUS       DASUTG010000270         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_636376, whole genome shotgun sequence.
ACCESSION   DASUTG010000270 DASUTG010000000
VERSION     DASUTG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636376"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF089_06155"
     CDS             <1..409
                     /locus_tag="VF089_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173580.1"
                     /translation="TRDDKNLPGVRYVGIKTTNPERLAEFYKFIFSMKEVDRMENART
                     ESRAIYLSDGTINLGLVMNPPVPREGLQVLGFQVNSIQQVEERLKEARGLTYKGEAPL
                     AIERNSFGGPYKTVSLKDPDGTHLDLSEEGWAG"
     gene            459..1454
                     /locus_tag="VF089_06160"
     CDS             459..1454
                     /locus_tag="VF089_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173581.1"
                     /translation="MHWVILNLIWVIGFFSVVSTAAGQTLKLNVSYTGVGPTQLPVWV
                     AKETGIFSRNGLDVQLIRAQAATSVMALLSGELNLIQGAAPAVVQSNLRGFGTVYVAA
                     GYVGFEYWLMSQRDIKSAEQLKGGLLGVSGLTGASATATQLILRKLGLVPGKDTSVIV
                     IGGTPERLIALRTGKIQATLLNPPTTFVAQREGFNVLADLGTLPFQNNGVVTTRKFIN
                     ENPEAVRRYVKSQIEAVHLMKTERATGLKVLAKYMGGTSDRELLEKSYDASVTEHTFP
                     AKQYPSQPGLKTVIDSLVADNPKAKEAKPEDFIDSQFIKELDERGFIDNLKSKKS"
     gene            complement(1868..2062)
                     /locus_tag="VF089_06165"
     CDS             complement(1868..2062)
                     /locus_tag="VF089_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX6173582.1"
                     /translation="MKRSTLLQHLRRYGCYLKREGSSHSLWTNPATGTVEAIPRHTEI
                     SNRLVRKICRNLSIPEIGQE"
     gene            complement(2059..2274)
                     /locus_tag="VF089_06170"
     CDS             complement(2059..2274)
                     /locus_tag="VF089_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6173583.1"
                     /translation="MKNEFTAIIERDGDWYIAYCPEIPGANGQGRTKDDARENLAEAI
                     ELILEDRREDALRGVPPDAIRETVTIK"
     gene            complement(2429..2554)
                     /locus_tag="VF089_06175"
                     /pseudo
     CDS             complement(2429..2554)
                     /locus_tag="VF089_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT          642 a          672 c          695 g          609 t
ORIGIN      
        1 cacgagggat gataaaaatc ttcccggagt aaggtacgtc gggatcaaaa ccactaatcc
       61 cgaacgtctt gccgaattct ataagtttat tttttccatg aaggaggtcg acagaatgga
      121 gaatgcgcga accgaaagcc gggcaatcta cctttcggat ggcacgatca atctgggctt
      181 ggtcatgaac ccaccggtac cccgcgaagg acttcaggtg ttagggtttc aagtgaacag
      241 cattcaacaa gtcgaggagc gtttgaaaga ggcgcgcggt ctgacgtaca aaggtgaggc
      301 gccgctggcg atcgaacgca actcatttgg cggtccctac aagaccgtct cgctaaaaga
      361 ccctgatgga acccatttgg atttgtcgga agaaggttgg gctggctaac gatgccaaat
      421 tcgtctcatg ttggtcatct aagcgaggag gcgcaacggt gcactgggtc atccttaatc
      481 tgatttgggt gatcggcttc tttagtgtgg tttcaacggc tgcgggacaa acgctcaagc
      541 ttaacgtcag ctataccggc gtcggtccga cgcagcttcc ggtgtgggtt gccaaagaaa
      601 ccgggatctt tagcagaaat ggtttggatg ttcagctgat tcgcgcgcag gctgcgactt
      661 cggtgatggc gcttctctcg ggagagctga atttgattca aggtgctgct ccggcggtcg
      721 tgcagagtaa cctgagaggc ttcggcaccg tctacgtcgc ggccggatac gtcgggttcg
      781 aatactggct gatgagccaa cgcgatatca aatcggcgga gcaactcaaa ggcggattgt
      841 taggcgtttc cggacttacc ggcgcttcag ccacggccac ccaacttatc ctgcgtaaac
      901 ttggactggt acccggtaaa gatacttccg tgatcgtcat cggtggcacg cctgaacgcc
      961 tgatcgctct caggaccgga aaaatacagg cgactttgct caacccgccg acaacttttg
     1021 tggcccagag agaagggttc aatgtgctgg ccgatttggg cacactgccg tttcagaata
     1081 atggcgtggt gacgacccga aaattcatca acgagaatcc cgaagcggtg agacgctatg
     1141 tcaaatcgca gattgaggct gtacatctga tgaaaaccga acgagccact gggctcaagg
     1201 tcctggcaaa atacatgggc ggcacgtccg atcgagaact cctggaaaag agttacgatg
     1261 cctcagttac ggagcacacg tttccggcaa agcagtatcc gtcgcaaccg ggcctcaaga
     1321 ccgtaattga ctcgctggtg gccgacaatc ccaaagccaa agaggcaaag ccggaggatt
     1381 tcatcgacag ccaattcatc aaagagctcg atgagcgcgg cttcatcgac aatctaaaaa
     1441 gcaaaaagtc gtgaactacg tttcggatcg atcaagaaag gtgagaacga gtgaacatca
     1501 aacaggtaag agacaaattg aacgtgatgg tcaatgaccg ctacaatacc ccggagattt
     1561 tgcggttttt tcctttccga tcactctccc caagaccgac aatgacttcg gccatctgct
     1621 aatctcggcg cgtttccgct cagccaattg tggcaataac tggtccgagg cttccgtttc
     1681 aaagattacg cggacagtcc gacaactatc atgctcgcca ccctccgaga aggcgaggac
     1741 atctgcgtta atcattttgt tacaattgtt caggtctgca ccctgacggg attagctaag
     1801 ccgtcgctag acgagcgaac ttcaagcgct ttacctatcg gtcttcagcc tgccggtcag
     1861 acagaactta ttcttgtcca atttctggta tcgataaatt gcggcagatc tttcgaacaa
     1921 gtcgattaga aatttctgtg tgccgaggaa ttgcttccac ggtgccggtc gctgggttcg
     1981 tccacaacga gtgagaactt ccttctcgct tgaggtagca accgtacctg cgaagatgct
     2041 ggagcagagt gctccgtttc atttgatcgt aacggtctcc cgaatcgcat ccggcggaac
     2101 tccacggagc gcatcctctc gccggtcctc aagaattagt tcgatagctt cggctaaatt
     2161 ttctcgagcg tcatctttcg ttcggccctg cccgtttgcg ccaggaattt caggacaata
     2221 ggcgatgtac caatcgccat cgcgttcgat gatggcggta aactcatttt tcataagccc
     2281 cctcagtcaa ttaagcagac tgcaggtcca tttgtctgtc taacccatgg gcctgcgccg
     2341 tgccgaataa gcgaacctaa aaatgaacat tttcgacatc gtttctttaa acggcgagtt
     2401 agctgcatat ccccaagcgc tgcggccttg tcatgcgggg ctgagccaat gctgactcaa
     2461 gagcatccag attgaggacg cctaaggctc ctccagattg actgatgact cggcgatgca
     2521 attcgagcgc ttcactcaaa gttagatagc gcactaggcg agacgccgat agagttcggc
     2581 gtttttgtgg aggatgtagt cggctgcctt ctgaaacg
//
LOCUS       DASUTG010000271         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_636449, whole genome shotgun sequence.
ACCESSION   DASUTG010000271 DASUTG010000000
VERSION     DASUTG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636449"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VF089_06180"
     CDS             complement(<1..1074)
                     /locus_tag="VF089_06180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6173584.1"
                     /translation="MKEAVIVEAVRTPMGRHSGILKDIRTDDLGAYIIAKLVERTGIN
                     KEEIEDVYFGCTNQAGEDSRNVARNASLLAGLPYTIPGATVNRLCGSGLEAINQAGRA
                     VETDHGDLFVAGGVESMTRGPWVMGKPATSFQRGDVTMYDSSLGWRFPNKRMGELYSL
                     INNGETAENVAEKYHISREDQDTFALSSHKKALEAQDQGLLRDEIIAVEIPQRKGAPL
                     VHDKDEGPRADTSLEKLAALQPAFRKGGSVTAGNSSPLSDGAAALLIATPEKAEQLRL
                     KPLARIIASAAAGVHPNYMGIGPIPAAQKAMKRAGLTIDQIDLVEINEAFASQVLACA
                     RELGIDLNKLNVNGGAIALGHPLG"
     gene            complement(1156..1905)
                     /locus_tag="VF089_06185"
     CDS             complement(1156..1905)
                     /locus_tag="VF089_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6173585.1"
                     /translation="MSTTKSSLAILGTNRLAQDWIALSKAGGLDAELVSDAVHIEANA
                     SLVIDTSAGNEAMKRRTLEKLDSGLAPSAVILTSCLGGSTTLMASWTKRPERVVGFAT
                     FYPLAARKIIEIAAGLRTSDSALQQAQDHFGALGKETVKVKDAAGLTFPRILSLIINE
                     AARSLEEGVAKADEIDVAMRLGVNYPMGPLRWADQIGLDEVLAVLEGLERETGDDRYR
                     PAPVIKKLVIAGFLGETTGRGFYSYEEQVKS"
     gene            complement(1886..2752)
                     /locus_tag="VF089_06190"
     CDS             complement(1886..2752)
                     /locus_tag="VF089_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6173586.1"
                     /translation="MTNPTEKIAVLGAGTMGAGIAQVAAQAGFDTLLYDIKQEFIDTG
                     LGRIRGFLKGSRERGKLSPEQEQQILDRLRSTTRLEDCQGSVLVIEAAPENLELKRDI
                     FKQLDSICGPETLLASNTSSFNVTAIAASTKHQERVLGLHFFNPPPLMALVEVVQGDR
                     TSPAIIDKATDLMQRMGKTPARAKDTPGFIVNRIARPFYNEALRILGDGDATVETIDR
                     VMKEAGNFRMGPFELMDLIGNDINFAATDSLYQSFFHDPRFRPSPLQQRMVMGGNLGR
                     KTGRGFYVYDKK"
     gene            complement(2809..>3115)
                     /locus_tag="VF089_06195"
     CDS             complement(2809..>3115)
                     /locus_tag="VF089_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6173587.1"
                     /translation="MELLLLGETVDAHEAQRIGLVAKVFPTAEFADSARNLAARVAGA
                     PRGIGLIKRAVNHANLPDLESDLEYEACLQEIAGRSSDYDEGVRAFLEKRTPTFTGK"
BASE COUNT          609 a          923 c          846 g          737 t
ORIGIN      
        1 tccgagagga tgtccgagag cgatagcgcc gccgttgacg tttagcttgt ttaagtcaat
       61 gccgagctcg cgcgcgcagg cgagcacctg tgaagcgaag gcttcgttga tctcgactaa
      121 atcgatctga tctatagtga gcccggcgcg tttcatggct ttctgagccg ctgggatcgg
      181 cccgatgccc atatagttag gatgaacgcc cgccgccgcc gaagcgatga tacgcgccag
      241 cggcttgagc ctcagttgct cggctttttc cggggtggca attagcaggg ccgccgcgcc
      301 atccgagagc ggcgatgagt tgccagccgt caccgaaccg ccctttctaa aggccggctg
      361 caaagccgcc agcttttcga gcgatgtatc ggcgcgcggt ccttcgtctt tgtcatgcac
      421 gagcggtgcg ccttttctct gtggaatctc aaccgcgatg atctcgtccc gaagcagccc
      481 ttgatcctgc gcttctaacg ctttcttatg gctcgataac gcaaaggtgt cttggtcctc
      541 gcggctgata tggtatttct cggcgacatt ctccgcggtc tcgccgttat tgatcagtga
      601 gtaaagctcg cccatgcgct tattgggaaa gcgccagccc agcgagctat cgtacatagt
      661 cacgtcgcca cgctggaaag atgtggcggg tttccccatg acccatggcc cgcgggtcat
      721 gctttcgaca ccgccggcca cgaacaggtc tccatgatcg gtttcgactg cacggcctgc
      781 ttgattgatc gcctccaggc ccgagccgca cagccggttg accgttgcgc ccgggatcgt
      841 atacggaagc ccagccaaca gtgaagcatt gcgcgcgaca ttgcgcgaat cttcccccgc
      901 ttggttggtg cagccgaaat aaacgtcttc aatttcttcc ttgttgatgc cggttctttc
      961 gacaagcttg gcgatgatgt aggcgccgag gtcatcagtc cggatgtctt taaggatgcc
     1021 gctgtgtcgc cccatcggcg tgcgcacggc ttcgacgatt acggcttctt tcatgatttc
     1081 acctgatttt cattatagta ggatgctggt gtgaagaccc gatggccgcg cgcacggcat
     1141 ctaatacgaa ttctttcatg atttcacctg ttcttcataa gagtagaacc ctctgccagt
     1201 cgtttcgccg aggaagccgg ctataaccag ctttttgatg accggcgcgg gacgatagcg
     1261 gtcatcgccg gtttcgcgct cgagaccttc aaggacggcg agcacttcgt ctaaaccgat
     1321 ctgatccgcc caacgcagcg gccccatcgg ataattgacg ccgaggcgca tcgccacgtc
     1381 gatttcgtca gctttagcca caccttcttc gagactacgc gctgcctcat tgataattaa
     1441 actcagaatt cgcggaaaag tcagcccggc agcatctttc accttaaccg tttccttgcc
     1501 tagcgctcca aaatgatcct gcgcctgctg cagagcgctg tctgaggttc gaagtcccgc
     1561 ggcgatttcg atgatctttc ttgcggcaag cgggtaaaaa gttgcaaagc cgactactcg
     1621 ctcgggccgt tttgtccaag acgccatcag agtagttgag ccgcccagac atgatgtgag
     1681 gatcaccgca gacggcgcta acccgctgtc gagcttttcc agggtacgtc ttttcattgc
     1741 ctcattgccg gccgaagtat cgatcaccag cgatgcgttc gcttcaatat gtacggcatc
     1801 gctcactagc tccgcgtcca ggcccccggc tttgctgagt gcaatccagt cctgcgccag
     1861 ccggttggtg ccgaggatcg ccaagctact ttttgtcgta gacatagaac cctcgcccgg
     1921 tcttgcggcc caagttgccg cccatcacca tacgctgttg gagcgggctc ggacgaaacc
     1981 tcggatcgtg gaaaaatgat tgatagagcg agtcggtggc ggcaaagttg atgtcattgc
     2041 cgatcaggtc catcagctcg aaaggtccca tgcggaaatt gcccgcttcc ttcatcacgc
     2101 gatcgatagt ttccaccgtg gcatcgccgt cgcccaggat gcgcaatgcc tcattgtaga
     2161 aagggcgggc gatgcgattg acgatgaagc ccggcgtgtc tttcgcgcgc gccggcgtct
     2221 tgcccatgcg ctgcattaaa tccgtggcct tgtcgatgat agccgggctg gtccgatccc
     2281 cttgcacgac ttcgaccaac gccatcagcg gcggcggatt gaaaaaatgc agacccagca
     2341 ctcgctcttg atgcttcgta cttgccgcaa tagcggtgac gttaaacgaa gatgtgttgc
     2401 ttgccaggag tgtttccggc ccgcaaatcg aatcgagctg tttgaagata tcgcgcttga
     2461 gttccaggtt ttccggagcc gcctcaatga ccaacacgct tccttgacag tcctcaagcc
     2521 gagtggtgct gcgcaaccga tcgagaatct gctgctcctg ctccggagaa agctttccgc
     2581 gctcgcggct gccctttaaa aagccgcgaa tacgacccag tccggtgtca atgaactcct
     2641 gtttgatgtc atagagcaga gtgtcaaatc ctgcctgagc cgcaacctgt gcgattcccg
     2701 cccccatggt gccggcgccg agcactgcga ttttttctgt tgggttggtc atatgctatg
     2761 ataaatccag ttgccggttc gatcgcgcac accggcccac tatgaatttt atttcccggt
     2821 aaatgtcgga gtgcgttttt ctaagaacgc gcgtacgcct tcatcgtaat cagagcttcg
     2881 cccggcgatt tcctgcaagc aggcttcgta ctcgagatcg gattcaagat ctggcaaatt
     2941 ggcgtgattc accgcccttt tgatgagccc gatgccgcgc ggcgcgccgg caactctcgc
     3001 ggccaagttc ctggctgaat cggcaaactc agcggttgga aatacttttg cgaccagccc
     3061 gatccgttgc gcctcgtgag catcgactgt ctcgcccaga agcaatagct ccata
//
LOCUS       DASUTG010000272         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_487136, whole genome shotgun sequence.
ACCESSION   DASUTG010000272 DASUTG010000000
VERSION     DASUTG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487136"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF089_06200"
     CDS             complement(<1..544)
                     /locus_tag="VF089_06200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6173588.1"
                     /translation="MATLKIIRKRITSIRNTQQITKAMKMVSAAKLRRAQEAALLARP
                     YADKMNDILNNLSARVSRAAHPLLAAREEKRIQLVLVTSDRGLCGGYNANLIRAAEAF
                     IRTHGNGKEILLALVGRKGADYYRRRRGETGERYLNFLATPAEELAVVIAEKLITRFV
                     DGETDAVYLLYSHFRSALSQV"
     gene            complement(544..2052)
                     /gene="atpA"
                     /locus_tag="VF089_06205"
     CDS             complement(544..2052)
                     /gene="atpA"
                     /locus_tag="VF089_06205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6173589.1"
                     /translation="MEIGTAEISRIIKEQIRDYEKTVEVQEIGTVLSTGDGIARIYGL
                     DRVAAGELLEFPHNIYGLTLNLEEDNVGAALFGETHMIREGDTVKRTGRIAEVPVGKP
                     LIGRVVNALGEAIDGRGPIETTERRRIEIKAPGIVARQPVKEPLQTGLKAIDAMIPIG
                     RGQRELIIGDRQTGKTAVAVDTIINQKGGNVICIYVAIGQKRSTVAQVVDKLREAGAM
                     DYSIVVAATASDSAPLQYIAPYSGCTIGEYIRDNGGHALVIYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKMSDDMGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQIKAMKQVAGTLRLELAQYREM
                     AAFAQFGSDLDQTTQRQLNRGSRLVELLKQGQYEPLPVEKQVLIIFAGTNGFIDDLPL
                     TAIKKYEQELFSFVESKYPDVFADILKKREMDADLRAKTMKVLEEFKLAFKP"
     gene            complement(2064..2606)
                     /gene="atpH"
                     /locus_tag="VF089_06210"
     CDS             complement(2064..2606)
                     /gene="atpH"
                     /locus_tag="VF089_06210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6173590.1"
                     /translation="MIEDSLSRRYSKALFELAQESGQEESIGDELERFSGASTESRLG
                     AVLSNPGFALEDRKKILLEVAKTIELSSITEHFLSLLLDRDRLAFVPAIIASYRHLLN
                     QAKGRVDAKVTAAEALGSVVIERLRESLGQLSGKEVILQQETNPELIGGLVVEFQGKI
                     YDGSVRTQLEKMKQRIARGF"
     gene            complement(2593..>3051)
                     /locus_tag="VF089_06215"
     CDS             complement(2593..>3051)
                     /locus_tag="VF089_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173591.1"
                     /translation="LNFLIYAGIIYYFAVPLIRSFLKSRHDEVLATVTGAAARKQSAE
                     SLVEDYRSRLARLNQEIDSIQASLKAEGERDKARLLREAEALAAKVKSDAQLLANQEV
                     KAAKHQVLLELAERAKASATDLIRRHLSQADQSKLVEEFIKDLGQVDDRR"
BASE COUNT          671 a          875 c          767 g          738 t
ORIGIN      
        1 gcacttgcga taaggccgaa cggaaatggc tataaagcag gtaaaccgca tcggtttcac
       61 catcgacgaa acgagtaatc agcttctcgg cgatcactac cgctagctct tcggcaggag
      121 tcgctagaaa attcaagtaa cgctcaccgg tttcgccccg gcggcggcga tagtagtccg
      181 ctcctttgcg cccgaccaaa gccagcaata tttccttgcc gttaccgtgc gtgcggatga
      241 aggcttcggc ggcgcgaatg agattggcat tataaccgcc gcacaaaccg cgatcggaag
      301 tgacgagcac caattgaata cgtttttctt cacgcgctgc cagcagcgga tgggctgctc
      361 gcgacacccg tgcagaaaga ttatttaaaa tatcattcat cttatccgca tagggacgcg
      421 ccagcagcgc ggcttcctgg gctcggcgca gcttggcggc agagaccatc ttcatcgctt
      481 tggtgatctg ctgcgtgttg cggatcgacg tgatccgttt acgaatgatt tttagggtcg
      541 ccattacggt ttgaaagcga gcttaaactc ttccagcacc ttcatggttt tggcgcgcag
      601 gtccgcatcc atctcccgtt tcttgagaat atcggcgaaa acatcggggt acttcgattc
      661 aacgaatgag aacagctctt gctcgtattt cttaattgcc gtcaacggga gatcgtcaat
      721 gaaaccattg gtgccggcga aaatgatcaa cacttgcttt tcgaccggca gcggctcgta
      781 ttgaccttgc tttaacagtt cgacaagccg gctgccgcga ttgagctgcc gctgggtcgt
      841 ctggtcgaga tctgatccga actgtgcgaa ggctgccatt tcacgatact gcgcgagctc
      901 cagccgcaga gtaccggcaa cttgcttcat agccttgatc tgcgcgttac cgccgacccg
      961 ggagacagaa attccgacgt taatcgccgg tcgaacgccc gaatagaaaa gatcgctttc
     1021 caaaaagatc tgtccgtcgg tgatggaaat gacattggtc ggaatgtacg ctgaaacgtc
     1081 gcccgcctgg gtttcaatga tcggcagcgc cgtaagtgat ccggcgccca tgtcatcgct
     1141 catctttgcg gcgcgttcca acagacgaga gtgaagatag aaaacgtcgc cgggataagc
     1201 ttcacgtccg ggcggccggc gtagtaacaa agagagctgc cgatacgcca ccgcatgttt
     1261 cgacagatcg tcgtaaatca ccagtgcgtg gccaccatta tcgcgaatgt actcgcctat
     1321 ggtacagccg ctgtagggag caatatactg aagcggcgcc gagtccgatg cggtggctgc
     1381 gaccacgatg ctgtagtcca tcgccccggc ctcgcgcagc ttgtcgacca cttgcgcgac
     1441 cgtggagcgc ttctgcccta ttgcaacgta aatacagatt acattaccgc ctttttgatt
     1501 gatgattgtg tccaccgcaa ccgcggtctt gccggtttgg cgatcgccga taatcagctc
     1561 gcgctgcccc ctaccgatcg ggatcatcgc atcgatggcc ttcaatccgg tttgcagcgg
     1621 ctccttcacc ggctggcgtg caacgattcc gggagctttt atttcgattc gccgacgctc
     1681 agtggtctca atcgggccac gaccatcgat cgcttcgccc aaggcgttga cgacgcgccc
     1741 gatcagcggt tttccgaccg gtacctccgc gatacgtccg gtgcgtttaa cggtgtcgcc
     1801 ctcgcgaatc atgtgagtct cgccgaatag cgcagcaccg acattgtctt cctcaaggtt
     1861 aagcgtcaag ccgtatatgt tatgtgggaa ctcgagcaac tcaccggcgg cgacccgatc
     1921 caagccgtaa attctagcaa tgccatctcc ggttgaaagc acagtgccga tctcttggac
     1981 ctcaaccgtt ttttcgtagt cacgaatctg ctcttttatg atccggctga tctccgctgt
     2041 tccgatctcc atttaaaact ccttcaaaaa ccgcgcgcga tgcgttgctt catcttctcg
     2101 agctgcgttc gcacacttcc atcgtagatt ttgccctgaa attcgaccac taggccaccg
     2161 atgagctcag gattggtttc ctgttgcagg atcacttctt ttccggacag ctggcccaat
     2221 gattcacgca aacgctcgat aaccaccgat cccaaagctt ctgcagcagt tactttcgca
     2281 tcaactcgtc ccttggcctg attcaaaagg tgccggtagc tggcgatgat cgctggcaca
     2341 aaggccaggc gatcccgatc gagcaaaagt gacaaaaaat gttctgtgat agaagaaagc
     2401 tctatggttt ttgcaacctc caacaggatt ttctttcgat cctccagagc gaaccccgga
     2461 ttactcaaaa cagcaccgag acgcgactca gtggatgctc ccgaaaacct ctccagttca
     2521 tctccgatgc tctcttcctg ccccgattct tgggcaagtt caaagagcgc cttggagtat
     2581 ctccgactta aactatcttc gatcatctac ctgcccgagg tctttaataa actcttccac
     2641 cagtttgctc tgatcagctt gtgacaaatg gcgccgaatc agatctgtgg cactggcttt
     2701 ggctctttcg gctaactcga gcaacacctg gtgcttcgca gccttgacct cttggttggc
     2761 gagaagctga gcatcgctct tgaccttggc agcgagagct tcggcttctc tgagcaagcg
     2821 cgctttgtct cgttcgcctt ccgccttcag tgaggcttgg atcgagtcga tctcctggtt
     2881 gagtctagct agacggcttc gataatcctc aactaaactt tccgcgctct gctttcgtgc
     2941 cgccgcgccg gtgaccgtgg ccaaaacttc atcatgccta gatttcaaaa agctacggat
     3001 cagtggaact gcgaaataat aaatgatccc ggcataaata agaaagttca g
//
LOCUS       DASUTG010000273         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_636560, whole genome shotgun sequence.
ACCESSION   DASUTG010000273 DASUTG010000000
VERSION     DASUTG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636560"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF089_06220"
     CDS             complement(<1..408)
                     /locus_tag="VF089_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173592.1"
                     /translation="MFDKITARSTGFFYGWWMVAVGCGMRMLGGGFHLYGFTIFFLPI
                     TQELGLSRAATSLVFSLARAEGAIEGPLAGYLIDRFGPRPMMLAGVILSGIGYMLLAG
                     VESYYGLIAVYLGVISLSFSAGFMHSPMVLANSW"
     gene            723..1730
                     /locus_tag="VF089_06225"
     CDS             723..1730
                     /locus_tag="VF089_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX6173593.1"
                     /translation="MKRHKITLIPGDGIGPEVMKPTLKLIKAAGVNIEWDSHLAGADA
                     LKKHGTTLPKQMMDSFAKNRVALKGPVTTPVGEGFASVNVELRQSFDLYANLRPIKNL
                     PGVKTRYENVDLIVVRENTEGLYSGIEHEVVPGVMESLKIITEKASTRIARFAFNYAV
                     ANGRKKIAAVHKANIMKLTDGLFLECARNMAQKYKKIAFSDLIVDNACLQLVLDPTRL
                     DVLLLENLYGDIVSDLAAGLVGGLGVVPGANLGDTHALFESVHGSAPDIKGKNIANPT
                     AIILAAVMMLEYISETKAAACLRAALHAVLTRGERLTVDLGGSASTSQFADAIRREME
                     K"
     gene            1760..>2233
                     /locus_tag="VF089_06230"
     CDS             1760..>2233
                     /locus_tag="VF089_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6173594.1"
                     /translation="MWFDIPAQIIALAAALSYAASGIAAKRGLRYSTPITVTLVSVTI
                     HAVTLSAAVLLTGGIPAVSWWVLFLFALTGTLQPIIRLFTYAGIHYVGASRGTTLRGA
                     HPLFSTALAIAFLGEQATLPVIAGTVLIVGGITLISWQTEAQRKTFRWWHIGYPLG"
BASE COUNT          529 a          607 c          616 g          481 t
ORIGIN      
        1 ccagctgttg gcgagaacca tgggcgaatg catgaacccc gccgaaaagg ataatgagat
       61 cacgcccaga tagacggcga tcaagccgta atagctttcg actccggcga gcagcatgta
      121 accgatgccc gacaagatca ccccggccag catcatcggg cgcggcccga aacgatcgat
      181 taagtaaccc gccagcgggc cttcgatggc cccttcggct cgcgctaatg agaagaccag
      241 agaagtcgcc gcgcgagaca atcccagttc ttgggtgatc ggcaagaaga aaatcgtaaa
      301 gccgtagaga tgaaacccgc cgccgagcat gcgcatgccg caaccgacag ccaccatcca
      361 ccagccgtaa aaaaatcccg tcgatcgagc cgtgattttg tcaaacattt aatgagatgg
      421 tccggctata cgacagatat cttcaggttt cgtggagctg gcacacctga agcggttaac
      481 cgcgcaagga tagtttgatg tgagttctct ttcgataatg ttcacgctag ctgagggtga
      541 acaataatgt caacgtctgg acggcttgta aacgatctcg ctgctatttg gcgctcgatc
      601 atgtataaaa tgatggatta tcagattgct gaaaattctg gttgccggcg ctcaaaaaga
      661 tccagaggcg agcgcaacta gtatatgggt ctttcagaag ctgcaatgga aggaacgtgc
      721 gagtgaaaag acacaagatc acattgatac ccggtgatgg aatcgggccc gaagtgatga
      781 aacccacttt gaaactgatc aaagctgcgg gagttaacat cgaatgggac agtcatctgg
      841 ccggggccga cgctttgaaa aaacacggca cgacgttgcc gaagcagatg atggactcgt
      901 ttgccaaaaa cagagtggct ctcaagggtc cggtgacaac gccggtcggc gaaggctttg
      961 ccagcgtcaa tgtcgagcta cgccagagtt tcgatctcta cgcgaatctc aggccaatca
     1021 aaaatcttcc cggtgtcaaa acccgctatg aaaatgtcga tctgatcgtg gttcgcgaaa
     1081 acaccgaggg cttatactcg ggcatcgagc atgaagtggt gcccggcgtg atggagagcc
     1141 taaaaatcat cactgaaaag gcctctacgc gaattgccag gtttgctttc aactatgcgg
     1201 tcgccaacgg tcgaaaaaag atcgccgcag tgcacaaggc caacatcatg aagctcaccg
     1261 acggcctttt tctagaatgc gcgagaaaca tggcgcaaaa atacaagaag atcgccttta
     1321 gcgatctcat cgtcgacaat gcctgcctgc aattggtttt ggatccgacc cgtctggatg
     1381 ttctcttact ggagaatctt tacggcgata tcgtctcgga tctggcggcc gggttggtcg
     1441 gcgggcttgg agtagttccc ggcgccaatc tgggcgatac ccacgcgctc tttgaaagtg
     1501 ttcacggcag tgcgccggat atcaaaggaa agaatatcgc caatccgact gcaatcatat
     1561 tggccgctgt catgatgctt gaatacattt cggaaacgaa ggcggcggcg tgtctccgcg
     1621 cggcgctgca cgccgtgctg acacgaggcg agcgtctgac ggtcgatctt gggggatcag
     1681 ccagcaccag ccaatttgcc gacgcgattc gccgcgaaat ggagaagtag tcttcgccat
     1741 tggcggcgct cccagcttca tgtggttcga cattccagct caaatcattg ccctggcggc
     1801 ggcgctttcc tacgcagctt cgggaatcgc cgccaagcgc ggcttgcgtt actctacccc
     1861 gatcaccgtc accctggttt cggtcacgat tcatgcggtg actttatcgg ccgccgtgtt
     1921 gctgaccggc ggtattccgg cggtatcttg gtgggtgctg ttcctcttcg ctcttaccgg
     1981 gacgcttcaa ccgatcattc ggctcttcac ctatgccggt atccattacg tcggagcatc
     2041 gcgcggcacg accttgcggg gcgcccatcc actgtttagc acagctctgg caattgcttt
     2101 tctcggcgaa caggcgactc tgccggtgat cgccggcacg gttctgatcg tcggcggaat
     2161 cacattgatc tcgtggcaga ccgaagccca acgcaagacg tttcgctggt ggcacatcgg
     2221 ttatcctctc ggc
//
LOCUS       DASUTG010000274         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1081315, whole genome shotgun sequence.
ACCESSION   DASUTG010000274 DASUTG010000000
VERSION     DASUTG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081315"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF089_06235"
     CDS             complement(<1..567)
                     /locus_tag="VF089_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173595.1"
                     /translation="MTGVSAAQAENPLPPGMYRLEMILASIARLPVFGLSKSASRSVS
                     LIEVRSNATGLLQRHEVCDFHVLQDSAMIKMVFPDKFVAALAQHSYPIQVEKDAEGWR
                     YRADLGIERIGYKPTSTESSLPTKVDDASVYDWDGDGHPGATLKISVPLLPDGELYVV
                     QRGQSILNGRITRPGRVEGTIEVRSFEHR"
     gene            complement(839..1312)
                     /locus_tag="VF089_06240"
     CDS             complement(839..1312)
                     /locus_tag="VF089_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6173596.1"
                     /translation="MKIYRLKTELWVSRPRVEVFQFFADPANLERLTPGWLRFKIISE
                     APQPMTEGARLDYRLRIHGLPIKWQSEITAWNPPHRFVDRQTKGPYQFWEHEHTFFER
                     EGGTLVCDNVQYSVLGGVLVNKFLVAPDLDRIFRYRHGVLNTIFNSPDENTRKRF"
     gene            complement(1309..1479)
                     /locus_tag="VF089_06245"
     CDS             complement(1309..1479)
                     /locus_tag="VF089_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173597.1"
                     /translation="MERHTNWYYLNGNSSVDCVGAGEEDQIEYCTIGIILEPTPQELL
                     LRTTLARPLVIR"
     gene            1573..>2233
                     /locus_tag="VF089_06250"
     CDS             1573..>2233
                     /locus_tag="VF089_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX6173598.1"
                     /translation="MKIAITGASGFIGGILADHLSTRRHHLVLLSRNPPRDANLTQQE
                     WIAWNPRAPGDWEQAIEGVDGIINLAGEPIAAKRWNDSQKEKIRSSRIESTRALVGAI
                     AKAKQKPKFLISSSAVGYYGAHGDETVTEETTPGNDYLSRVCVDWEKEASDAETYDVR
                     VVLVRTGIVLDKGKGALAKMVVPFKCFLGGPLGSGNQWMPWIHIEDQIALLLFLLENQ
                     NA"
BASE COUNT          552 a          579 c          578 g          524 t
ORIGIN      
        1 acgatgttca aagctgcgca cctcaatagt gccctcaacc cttccgggcc gagtgattcg
       61 gccgttaaga atcgattggc ctcgctgtac gacatacaac tctccgtccg gcagtaacgg
      121 cacggatatc ttcagggtcg ctcccgggtg cccatcacca tcccaatcat aaaccgaagc
      181 atcgtctacc tttgtcggca gactggattc ggtcgaagtc ggtttatagc cgattcgttc
      241 aatcccaaga tccgctcgat agcgccatcc ttcagcatct ttctcaactt ggatgggata
      301 ggagtgttga gccaatgcgg caacgaactt gtcgggaaac accatcttga tcatcgcgga
      361 atcttgcagg acatggaaat cgcatacctc gtggcgctgc agtaaaccgg ttgcattgct
      421 tctgacctcg atcaatgaga ccgatctgga agcggatttg gataggccaa aaactggcaa
      481 gcgtgctatc gatgccaaaa tcatctccag acgatacatg ccaggcggca ggggattctc
      541 tgcttgtgcc gcggaaacgc cagtcaaaat caggctgctt agcaggagca ctacgaagca
      601 tgaacccatg cagcacgggc tgttttcatc ttgtttttga ctccacatag tgacgatggc
      661 caccgctagc taaatccttt ggactcttac cagatcgaga ttcgatgcgc taatctgtcg
      721 atcatccgag cggatgctcg gttcatgaac tgttgatgcg ttggagttcc cgatccgaat
      781 gacatcccgt tgcacttgca ttggcttgtt gatgacgatt gcgcgtcaaa ttagaacatt
      841 agaacctctt gcgagtattt tcatccgggg aattgaagat cgtgttcaaa accccgtgac
      901 ggtatctgaa gattcgatcc agatccggcg cgactaaaaa tttgttcacg aggacgccgc
      961 ccaatacgga atattggacg ttatcgcata ccaaggtgcc gccctcacgc tcaaagaagg
     1021 tatgctcatg ctcccaaaac tggtaagggc ccttagtctg ccggtcgaca aatcggtgcg
     1081 gcggattcca agcggtaatc tcgctttgcc acttaatcgg caagccatgg atgcgtaacc
     1141 ggtagtccaa ccgtgctcct tctgtcattg gttgcggggc ttcgctgatg atcttaaagc
     1201 gcagccaacc gggcgtgaga cgctcaagat tagccgggtc ggcgaagaac tgaaagactt
     1261 caacgcgcgg tcgagaaacc caaagttcgg ttttcagccg gtaaattttc atcggatcac
     1321 cagcggtcgc gcaagcgtgg tgcgcagcag tagctcctgt ggcgttggct ctaaaatgat
     1381 tccgatcgtg caatactcta tctggtcttc ttcacctgca ccgacacaat caacagacga
     1441 gttgccgtta agataatacc agttggtgtg tcgctccatg gcattgcact ccgtctccat
     1501 tatccgaagc attgatcctg acgaggtcaa ctatacttga ctttagaagg tgaccctacg
     1561 agagtaaagc agatgaaaat tgccatcacc ggagccagtg gcttcatcgg cggtattctt
     1621 gccgatcatc tctcgacccg gcgccaccat ctcgtactgc tcagccgtaa cccgcctcga
     1681 gacgcaaatc tgacgcaaca agaatggatc gcctggaatc ctcgcgcacc cggtgattgg
     1741 gaacaggcga ttgagggtgt ggacggtatc ataaacctcg caggcgagcc tatcgcggcc
     1801 aaaagatgga acgattcgca gaaggagaaa atcagatcta gccgcattga gtcgaccaga
     1861 gctctggtcg gagcaatcgc taaagctaaa cagaaaccaa aatttctcat cagctcctct
     1921 gcggtaggtt attacggagc ccatggggat gagaccgtca cagaagaaac gactccgggc
     1981 aatgattact tgtcgcgcgt ttgtgtcgac tgggaaaagg aagccagcga cgctgaaact
     2041 tatgatgtgc gggtggtgtt agtgagaacc ggcatcgtat tggacaaagg taagggagcg
     2101 ctggcaaaaa tggtcgttcc gtttaagtgt tttctcggcg gtcctctcgg atcaggtaat
     2161 caatggatgc catggattca tatagaagac cagattgcct tgctgttgtt tcttctggaa
     2221 aaccagaatg ccc
//
LOCUS       DASUTG010000275         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1823467, whole genome shotgun sequence.
ACCESSION   DASUTG010000275 DASUTG010000000
VERSION     DASUTG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823467"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VF089_06255"
     CDS             complement(<1..120)
                     /locus_tag="VF089_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX6173599.1"
                     /translation="MIFNETKLKGAYIIQVEFSEDERGSFARTFCREEFERHGL"
     gene            complement(171..1628)
                     /gene="rfbG"
                     /locus_tag="VF089_06260"
     CDS             complement(171..1628)
                     /gene="rfbG"
                     /locus_tag="VF089_06260"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX6173600.1"
                     /translation="MNADFWQSKRVLITGHTGFKGSWLSLWLQSLGAHVVGYALDPPT
                     EPNLFDTARVAERMLSIPGDVLNKSALQSVIAEHRPEVIFHLAAQSLVRESYKNPVET
                     FSTNVMGLAHLLEGVRTIGGVRAVVCVTSDKCYQNREWVWGYREDEPMGGYDPYSSSK
                     GCSELVAAAYRNSFFNEREYSQHGVSVATARAGNVIGGGDWAKDRLVPDLIRSVIRGQ
                     DIVLRNPHAIRPWQHVLDPLAGYLMLAESLFKHGPRFSEAWNFAPAESHTVTQVVEQL
                     LSLWGSQVSAKHDQSRQPHEDPYMILDSTKARVKLEWKALLDLRASLSWIVEWVKRFQ
                     AGADMRSVTESQIKAYMDMVEISRTGDQAQNYRPAHRNNAELLDLLYETIMIRDREGR
                     IKYWNRGADEMYGWKSEEALGSISHELLRTKFPKSLGDIESDLTREGCWQGELVHTRR
                     DGRTIVVKSRWVLKQDGRSNDGEVVEINQICGKPE"
     gene            complement(1694..2785)
                     /locus_tag="VF089_06265"
     CDS             complement(1694..2785)
                     /locus_tag="VF089_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019495319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6173601.1"
                     /translation="MNILITGVDGYIGTVLAAYLITGGHTVTGLDTGFYRDGWLYNDH
                     ALQRPRCLHRDLRAVTENDVTGFDAVVHLAELSNDPLGQHNPELTYAINHKGSVALAT
                     KCMKNGVSRFVYTSSCSVYGTGSGEFKSEESETNPQTAYAKCKLLVEHDLSRLANDNF
                     SPTFLRNSTAYGPSPRMRFDLVLNNLGALAWTTQQIKMTSDGSPWRPLVHVRDIAQAI
                     SCVLEAPRSVIHNQVFNVGSNAENYQVKEIAEIVAEAFLGCALSFGKSDGDNRSYRVC
                     FDKINSLLPGFRCRYNARDGAFELYELFQRIDMTRDVFDFRAYTRLKQLEYLLRTGQI
                     DDQFYWVGSNDSRALSENQLGDHRARATG"
     gene            complement(2950..>3281)
                     /locus_tag="VF089_06270"
     CDS             complement(2950..>3281)
                     /locus_tag="VF089_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6173602.1"
                     /translation="FLEFLITAKNEGRSVVGYGAPAKGNTLLNFCGVRTDFIDYTVDR
                     SPHKQGQFLPGTRIPIHHPDRIMETRPDYVLILPWNLTDEITAQMSYIRTWNGRFVVP
                     IPEVKVL"
BASE COUNT          793 a          899 c          772 g          817 t
ORIGIN      
        1 tagcccatgc ctctcaaatt cttcgcgaca gaatgtgcgc gcgaacgatc cgcgttcgtc
       61 ctcggaaaat tcaacttgaa tgatataagc gcctttgagt tttgtttcgt taaagatcac
      121 tgaactgtaa catagccggc aaatcgatcg gcaatcgtga aaccgtttaa ttattcaggc
      181 ttaccgcaaa tttgattgat ctcgacgact tcaccatcat tgcttcgacc atcctgtttc
      241 aatacccagc gactcttcac cacgatcgtc ctgccgtctc tcctggtgtg cacgagctct
      301 ccttgccaac atccttccct ggtgagatcg ctttcgatgt cacctaatga cttgggaaat
      361 ttcgttcgaa gaagttcatg ggaaatactg cccagcgctt cttcgctctt ccaaccatac
      421 atctcgtcgg cgccccgatt ccaatattta attctgccct ctctatctcg aatcattatg
      481 gtttcataaa gcaagtcgag aagttcagca ttattgcgat gagctggcct gtagttttgg
      541 gcttgatctc cagtcctgct gatttcaacc atgtccatgt aggcttttat ttgcgactcc
      601 gtaacgctgc gcatatcagc gcctgcttgg aaccgtttaa cccattctac aatccaactg
      661 agactcgctc ttaagtccaa caatgccttc cattccaact tcacccgtgc cttggtacta
      721 tccaaaatca tgtaaggatc ttcgtgaggc tgacgactct gatcgtgctt ggcagaaacc
      781 tggctgcccc agagcgagag cagttgctca accacctggg tcactgtatg agattcggct
      841 ggtgcaaaat tccacgcttc actgaaacgt ggcccatgct tgaacagaga ctccgcgagc
      901 atcaagtaac cggcaagcgg atcaagcacg tgttgccaag gccggatagc atgaggattc
      961 ctcaatacga tgtcctgacc ccggatcacc gatcgtatta ggtcgggaac caggcgatct
     1021 tttgcccaat cgccgccacc gataacgttt ccagcccggg ctgtcgccac cgacactccg
     1081 tgctgcgaat attcacgttc gttaaaaaag gaatttcgat aggcggcagc caccagctcc
     1141 gagcacccct tgctgctgct gtacggatca tacccaccca tcggttcatc ttctcgatag
     1201 ccccaaaccc attcacgatt ttgatagcac ttatctgatg tgacacagac aaccgctcgc
     1261 accccaccga tcgttcgcac accttccaat aagtgcgcca ggcccatcac attggtcgaa
     1321 aaggtctcga ccggattctt atacgattca cggactagcg actgagccgc aagatgaaaa
     1381 atcacctcag gccgatgctc tgcgatcacc gactgcaaag cgcttttatt cagcacgtcg
     1441 ccggggattg aaagcatcct ctcggctact ctagcggtgt cgaaaaggtt tggttcggtt
     1501 ggcgggtcga gcgcgtagcc aaccacatgg gcgcccaagc tctggagcca aagcgaaagc
     1561 cagctccctt tgaagccggt gtgcccggtt ataaggaccc tcttagattg ccaaaaatca
     1621 gcgttcatat gaattcgttt tatctctgtg aaccgcgacc acgtagagta tctcagcaaa
     1681 aattgcgctc gagtcaacct gtcgcccgcg cgcgatggtc accgagttgg ttttctgaca
     1741 aagcacgcga atcatttgat cctacccagt agaactggtc gtcgatttgg ccggtgcgca
     1801 gcagatattc gagttgcttc aggcgtgtgt aggcccggaa atcaaacacg tcgcgcgtca
     1861 tatcgattct ttgaaacagt tcatacaatt caaaggcgcc gtcccgggcg ttatacctgc
     1921 atcgaaatcc cggcaatagt gaattgatct tatcaaagca aacgcgataa ctacggttat
     1981 caccatcgct ctttccgaac gacagcgcgc agcctagaaa ggcttcagcg acgatttcgg
     2041 caatttcttt tacctggtag ttctcggcgt tcgacccgac attgaagacc tgattatgga
     2101 taacgcttct cggggcttcc agaacgcagg agatggcttg agcgatatct cggacatgaa
     2161 caagaggacg ccagggactg ccatcgctgg tcatttttat ctgttgggtg gtccatgcca
     2221 aagcgccgag gttatttagc accagatcaa agcgcatccg tggcgagggt ccgtaagcag
     2281 tggagttacg caaaaacgtc ggcgaaaagt tgtcattggc aagtcgcgat aggtcatgtt
     2341 caactagcag tttacatttg gcataagccg tctgcggatt agtttcagac tcttccgatt
     2401 tgaactcacc ggatcccgtc ccatacacgc tgcatgatga tgtatagaca aaccttgaca
     2461 cgccattctt catacacttc gttgccagtg ccacgctgcc cttatggttg atcgcgtagg
     2521 tcaattcagg attgtgctgt cccaaagggt cgttggatag ctctgccaga tgaaccacgg
     2581 catcaaaacc ggtgacgtcg ttttcagtta ctgcgcggag atctctatgc aaacagcgcg
     2641 ggcgttgtaa ggcatgatca ttgtataacc agccatcccg gtaaaagccg gtatctagac
     2701 ctgtgacagt gtgcccccct gttatcagat aagccgctaa aaccgtacca atatagccat
     2761 cgacgccggt gataaggata ttcattgcgt tctcctgttg cgataagcgc cgctatttct
     2821 aaacctcatt atgcgagctt ctgaagcgtt cccgtgttca cttgagaacg gattagccgt
     2881 gcaaaggcaa atatcatgcg ccgggatgaa acccaagccg aacatcggcg gaatccgtac
     2941 gcgagcaagt tatagaacct ttacttctgg aatcggcacg acaaagcgcc cgttccaagt
     3001 tcggatgtag ctcatttggg cggtaatttc gtcggtgaga ttccaaggca aaatcagaac
     3061 gtaatctggg cgtgtttcca tgatccggtc cggatgatga atcggaatgc gtgtcccagg
     3121 caaaaattgg ccctgcttat gagggcttcg atcgacggtg taatcgatga aatcggtccg
     3181 gactccgcag aaattcagca gcgtattgcc ctttgccgga gccccgtaac ccaccaccga
     3241 acgtccctcg ttttttgccg tgatcagaaa ttccaagaac t
//
LOCUS       DASUTG010000276         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_637102, whole genome shotgun sequence.
ACCESSION   DASUTG010000276 DASUTG010000000
VERSION     DASUTG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637102"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1006)
                     /locus_tag="VF089_06275"
     CDS             complement(134..1006)
                     /locus_tag="VF089_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6173603.1"
                     /translation="MERHSETRRLKWALAISLGYFFAELIAGFLTNSLALLSDAGHML
                     SDIGAIVVSLFAFRMARRPATPESTYGYHRAEILAALFNGLALWLIVGVIFAAAYNRL
                     NSPPEVESLGMIIVATVGLMVNIAAGFILYDSHHHNLNLHGAFMHVISDAIGSVGAIV
                     AGLIMYFTGWYLADPLISVFIGLLILYSSWNLIREALSILMQSVPKTIRLEEVQRTIE
                     DVQGVMAVHDLHVWAVTSGIFTLSAHAVVINGEDFHEVLNRIEETLKEHFSIEHTTIQ
                     LETESREEMEFKGF"
     gene            complement(1099..2286)
                     /locus_tag="VF089_06280"
     CDS             complement(1099..2286)
                     /locus_tag="VF089_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX6173604.1"
                     /translation="MRNPFLALVGLLTWLALGIQIGDSQIVASTLPITDDLDRESLRR
                     AINHSISYLSKLQADRIVGEQPRQLSAGQVLDNLAALDRLLDRWNCWQCFVEEFKSRF
                     ELIPSSRDEQQREVLFTGYYQPVIEGSLVETREYRYPIYGKPTELVPLGGLAAGSGET
                     AKISGRVEGERFAPYYTRREIDHAQLLRGRGLEIAWVKDPVELFFLHIQGSGILQLPD
                     GERLHIGYAGQNGRPYRSIGRLLIDQGKISQTEMSMQRLRRHLTEHPEERDEIMAHNE
                     SYVFFRVTEAGPFGSLEVPVTAGRSIATDSRIFPKGAPALIYSESPVLNGAGHSIGWR
                     PFVRFVLNQDTGGAIRGFQRADLYMGSGADAGAHAGYMNSPGKIYFLMLKNLSGRGRA
                     LLR"
     gene            2333..2701
                     /locus_tag="VF089_06285"
     CDS             2333..2701
                     /locus_tag="VF089_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6173605.1"
                     /translation="MNDKELREEAARVLDELPDEFRERLHNIDIVVQKRPSKQQLAEM
                     DLDFHEEVIYGLYEGVPLPHRSAFDPPILPDKITLFSEPLLNDFPDLDELREQIRLTV
                     IHEIGHYFGMDDDEIEKLGY"
     gene            complement(2698..2871)
                     /locus_tag="VF089_06290"
     CDS             complement(2698..2871)
                     /locus_tag="VF089_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173606.1"
                     /translation="MAREKARAELPGREWIDGYDEWYRIGETSQNEWSAAMEQLYHSD
                     GFSVGKLIRPKRD"
     gene            complement(2912..3097)
                     /locus_tag="VF089_06295"
                     /pseudo
     CDS             complement(2912..3097)
                     /locus_tag="VF089_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT          760 a          881 c          798 g          694 t
ORIGIN      
        1 gtcagtgtag cgctaatggc actattgctg ccgtcaggca aatccgcgct ggctagccca
       61 agcgtctcct gagccgagcc gtttaacttg cggtcctttt aacaagatcc tctcccgcaa
      121 acttccgagt gcactaaaag cctttgaatt ccatttcttc gcggctctcg gtttccaatt
      181 gaatggtcgt gtgctcaata ctgaaatgct ccttgagggt ttcctcgata cggttcagga
      241 cttcgtgaaa atcttcgccg ttgatgacca cggcgtgcgc gctcagggta aagatcccgg
      301 aagtcacggc ccaaacatga agatcgtgaa ccgccatgac gccttgcacg tcctcgatgg
      361 tgcgctggac ttcttcgagc cgaatggttt tggggaccga ctgcataaga atactcaatg
      421 cttctctaat caagttccac gatgagtaaa ggatcagcaa tccgatgaac acgctgatca
      481 atggatcggc cagataccaa ccggtaaagt acatgataag cccggcgaca atggcgccta
      541 ccgagccgat ggcatcacta atcacatgca taaacgcgcc gtgcagattg agattgtggt
      601 gatgcgagtc gtagagaatg aatccggcgg caatgttcac catcaaacca actgtcgcta
      661 caatgatcat cccgaggctc tcaacttccg gtggactgtt gaggcggtta tacgcggcag
      721 caaaaatcac cccaacaatc agccagagcg cgaggccatt gaacagagcg gctaaaattt
      781 ccgcgcgatg atagccgtaa gtgctctccg gagtagccgg ccggcgcgcc attcgaaagg
      841 caaagagact gacgacgatg gcgcctatgt cggacagcat atgcccggca tcggaaagta
      901 aggccaagct attggtgaga aaaccggcga ttagttccgc gaagaagtag ccaaggctga
      961 tagcgagcgc ccacttgagg cgccgggtct cgctgtgtcg ttccatatcg aagtatttta
     1021 gtacgctagt cttccggccg gtagagtttc aagaacaaac cgcgccctgt ggatggaacg
     1081 gagaggccgc ctggctattt aacgaagtag cgctcgccct cgcccgctta ggttctttaa
     1141 catcagaaag taaatcttcc cgggactatt catatagccc gcgtgagcgc ccgcatcagc
     1201 accgctgccc atatacagat ccgcgcgttg gaaaccgcgg atcgccccac cggtatcttg
     1261 attgagcacg aaacgaacaa aaggccgcca gccgatcgag tgacctgctc cgttcaaaac
     1321 cggactctca ctatagatca atgccggggc tccctttgga aaaatgcgcg aatcggtcgc
     1381 aatcgatcgt cccgctgtca ccggcacctc gagactgcca aaaggacctg cttcagtgac
     1441 tcgaaaaaat acgtagctct cattgtgcgc catgatctca tcgcgctcct caggatgttc
     1501 cgtcaagtga cggcgcaaac gctgcatcga catctcggtt tgtgagattt tgccttgatc
     1561 gatgagcaac cggccaatgc tccgatatgg gcggccgttt tggccggcgt agccgatatg
     1621 cagccgctct ccatccggca gttgcaaaat gcccgagcct tggatgtgaa gaaaaaataa
     1681 ttccaccgga tccttgaccc aggcgatctc aagcccgcgc ccgcgcaaca attgagcgtg
     1741 atcaatctca cgccgagtgt agtaaggagc aaagcgctcg ccctcgactc gtccgctgat
     1801 ttttgcagtc tcgccggaac ctgccgcaag cccaccaagg ggcaccagtt ccgtcggttt
     1861 gccgtagatc ggatagcgat attcgcgggt ttcgacaagg ctgccttcga tgaccggctg
     1921 ataataaccg gtaaataata cttctctctg ctgctcatct cggctcgagg gtatcagctc
     1981 aaaacgggac ttaaattctt caacgaaaca ctgccaacag ttccagcggt caagcaggcg
     2041 atcgagagcg gcgaggttgt cgaggacttg tccggcgctg agttgccggg gttgctctcc
     2101 gacgatgcga tcagcctgca gcttgctcag atacgatatg ctgtggttga tggcccgccg
     2161 gagcgattca cggtcgaggt catcggtgat cggcagcgta gacgcgacaa tctggctatc
     2221 accgatttga attccgagtg cgagccatgt cagcaaaccg accagcgcta gaaatgggtt
     2281 ccgcaccaat actccttgaa ctcgatagcc gattcgtgca accattttac cattgaatga
     2341 caaggagcta agagaagaag cggcgcgcgt gctcgacgaa ttacccgatg aattccgaga
     2401 gcggttgcat aatatcgaca tcgtagtaca gaagagaccg agcaaacagc aattggcgga
     2461 aatggattta gattttcacg aagaagtcat ctacggactt tatgaaggag tgcctttgcc
     2521 gcaccgctcg gcattcgatc cgcctatctt gccggacaaa atcactctat tttccgaacc
     2581 cctgctcaac gactttcccg acctcgacga attacgtgaa cagatccgac tcaccgtaat
     2641 acacgagatt ggccactact tcggcatgga cgacgatgaa atcgagaagc tgggctacta
     2701 atcacgttta gggcgaatca gcttcccaac cgagaagcca tcggagtgat aaagctgctc
     2761 catcgccgcg gaccattcat tctgactcgt ctcgccaatt cgataccatt catcataacc
     2821 gtcgatccat tcgcgacccg gaagttcagc gcgggctttc tcgcgggcca tgggctgatt
     2881 caagagtgcc ggtggggtaa cgttcgacca cttaggtttc aatgccgatg agttccatta
     2941 tctttatctg atcaaatcca actaccgctt ccccatcgac gatcgttacg ggcgtgcttt
     3001 gataccccat atcgatcaac tctttcaacg ctgccttatc ggcgcgcacg tttttttcca
     3061 caaactcgac gcctttttga gatagaaact ccacctcctt gtggcagggc cctcaacccg
     3121 gctgcgtata gac
//
LOCUS       DASUTG010000277         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1823891, whole genome shotgun sequence.
ACCESSION   DASUTG010000277 DASUTG010000000
VERSION     DASUTG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823891"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1278
                     /locus_tag="VF089_06300"
     CDS             202..1278
                     /locus_tag="VF089_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6173607.1"
                     /translation="MGESEIEIANISEPTADEIKASGGDQKSLAAVAAVQEGNNFWRA
                     GEVEQSSLSYRRAIGLDPSLYSAQFNLGITLLHSKDYQRAAFAFTEALRLRPASVSAW
                     QSLGFAYYYGKRYHKAVEAFGEAQRLAPKDAVTNNNVGFAYLFVGRFEDAIVSFQTAL
                     QLDSGLGPAINGLCSAQALAKNAANAVEACLRATKSEGDSAAPRYFLGVSYMDLGQPE
                     KALSALQEAAGIEPRTARIHVGLGFACFKLKKYQEALKHFEHARKLNVKAKHAQLGLG
                     ATYAELKDYDKAEKALREAVSSDPDNPVAQFNLGIVCLARRNRDCALSQYNRLKMIDD
                     SFAKTLFTAMFQHRVVDASSYRKP"
     gene            complement(1393..3309)
                     /locus_tag="VF089_06305"
     CDS             complement(1393..3309)
                     /locus_tag="VF089_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6173608.1"
                     /translation="MVISDIPSGTITYPPIKDFGLIGDLHTAALVSKNGSIDWCCWPD
                     FDSPAVFCKLLDAEHGGSFAINPLKNFVSSQRYVEGTNVLQTVFETDSGRIRLSDFMP
                     VGRLAGYIEYPPILRRSISRLIEGVSGDVEVEVNFHPTFNYARSKTLIEPFDKGGTAR
                     STDETVVLYCPFALKANGDSSLRCRFKISNGDRVWLHLSYARDSKIDFQELAAADHEI
                     EFEQTIAHWREWLDLCTYEGDYAAFVHRSALVLKLLTFAPTGAIIAAPTTSLPETPGG
                     VRNWDYRYTWLRDSSLIVYALQLIGYNHEATAFLDWLNRLQIAEHKRVQIMYSVRGED
                     DLTEYHLEHLEGYHASQPVRIGNAAYQQKQYDIFGEVLDAIFLYHERVHKGLPKRWWD
                     EVRFLADEAARCWSLPDSGIWEFRAGERHFLYSKLLCWVALDRAIKLGIHRYDKKRFA
                     GWVRNRDQIREIILQEGFNQKIGAFTQLLGGNELDATALTIPLLGLLPATDERVKSTI
                     TQIEQKLSANGLVYRYLTDDGLPGREATFGICSFWLVDNLALAGRIDEARDLFERLTR
                     YAGDLQLFSEQIEPESGKFFGNYPQGFTHLALIRSAFHIAKAETMGAESVSHDPADRT
                     NQMEQSGRLPSATE"
     gene            3731..>4207
                     /locus_tag="VF089_06310"
     CDS             3731..>4207
                     /locus_tag="VF089_06310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6173609.1"
                     /translation="MKQKRATELINGVDTGRLFETVESIRKSPDLAVFRFRLNNNWLG
                     GGRNKSMIQNFYGAGEENSTRKKPFVIDADEPAVLLGSDKAPNPVEYLLHALAACVTT
                     SMVYHAAAKGIRITEVESRVEGKLDLRGFLGLSEDVPPGYENIQMLFKIKADVPDDQ"
BASE COUNT         1090 a         1039 c         1069 g         1009 t
ORIGIN      
        1 taatgcgatc tgctggcacg tacgttgcgg aacgcgaaaa ccagcctgct tgatgtattg
       61 aaccagaacc gttgagagag agagagagag agagagagag agagaggcgc catgaggatt
      121 ctgagatcgt ttctgattac gttcatagtt gtaagttttt ccaatgctca agagagcggt
      181 gatagatcag cttcgcaaga ggtgggagaa agcgaaatcg agatcgcgaa cataagcgaa
      241 cccaccgctg atgaaataaa ggccagcggt ggcgaccaaa aatccctcgc ggcagtcgcg
      301 gcagtgcaag aaggcaacaa tttttggcgc gcgggagagg tagagcaatc aagtcttagc
      361 taccggcgtg ctataggtct tgatccatca ctttattccg cgcagttcaa tctcggaata
      421 acgttacttc acagtaagga ctatcaacgt gcggcgttcg ccttcactga agcgctacgt
      481 ctgcgaccag catcggtatc tgcatggcag agcctgggct tcgcctatta ttacggcaag
      541 cgttaccaca aggcagtcga agctttcggc gaggcgcaac gattagctcc caaagacgcg
      601 gtaacaaata acaacgtggg cttcgcttat ttgttcgtag gtcgatttga agatgcgatc
      661 gtttcgtttc aaactgcatt gcagctcgat tcaggacttg gaccagcgat aaacggcttg
      721 tgcagcgcac aggccctcgc caagaatgcg gctaatgctg ttgaagcttg cctaagggct
      781 acgaagagcg agggtgattc agcagcgcct cggtattttc ttggcgtttc ctatatggat
      841 cttggtcagc ctgaaaaagc gctgagtgcc ttgcaggaag ccgctggtat tgaaccccgc
      901 accgccagaa ttcatgttgg cctgggattc gcctgtttta agctaaaaaa ataccaggaa
      961 gcattgaagc atttcgagca cgcgaggaag ttaaatgtaa aagcaaagca cgcccaattg
     1021 gggctgggag ccacttacgc agaacttaaa gattacgaca aagccgaaaa agcattgcga
     1081 gaagcagtgt cctcagatcc cgacaacccg gtggcgcaat tcaatctcgg aatagtatgt
     1141 ctcgctcgca gaaaccgtga ttgtgccctc tcacaataca accggttaaa gatgatagac
     1201 gattcgttcg ccaaaactct cttcaccgca atgtttcaac atcgagtcgt agacgcatcc
     1261 agctatagga aaccatgaac atgcgtttta ctcactgcat ctgcctggtc tctgagaaac
     1321 cagtcgaggg agtcgttttc tatttggttc tgttgtcgat cgtatggatt aacaagccgc
     1381 cactcgtgtc agttattccg tggcgctcgg aagccgaccg ctttgttcca tttggtttgt
     1441 ccgatcggca gggtcatgag agacgctctc tgcgcccatc gtttcggcct tagcaatgtg
     1501 aaatgccgat cgaatgagcg ccagatgtgt aaaaccttgc gggtagttac cgaaaaattt
     1561 tccgctttcg ggttcaatct gttccgaaaa cagttgtaga tcaccggcat atcgagtcag
     1621 ccgttcaaaa agatcgcgcg cctcgtctat tcgtcccgcc agcgccaggt tatccaccaa
     1681 ccaaaagctg caaatgccaa acgttgcttc tcttcccggc aaaccgtcat ctgttaaata
     1741 gcgatagacc aacccatttg cggagagttt ttgctcgatc tgcgtgatgg ttgatttcac
     1801 tcgctcatca gttgcaggaa gaagaccgag caggggaatc gtcagagctg ttgcgtcaag
     1861 ctcgttacca ccgagtaact gagtgaacgc gccgatcttc tgattgaagc cctcttgaag
     1921 aataatttca cgaatctgat cccggtttcg gacccacccg gcaaacctct ttttatcata
     1981 gcggtgaatg cccagcttga tcgcacggtc aagtgctacc cagcagagca acttcgaata
     2041 caaaaagtgc ctttccccgg cccggaactc ccatatacca ctgtcaggaa gactccagca
     2101 gcgcgccgcc tcgtctgcta aaaatcttac ttcgtcccac cagcgtttcg gcaacccctt
     2161 gtggacgcgt tcgtgataca aaaaaatcgc gtcaagcact tctccaaaga tgtcgtactg
     2221 cttttgttga tatgcagcgt tgccgatcct gactggctgc gaagcgtggt agccctcgag
     2281 gtgctcaagg tgatactcgg tgaggtcgtc ttcgccacgg actgaataca tgatctgcac
     2341 tcgcttgtgt tccgcaattt gcagtcgatt cagccagtcc agaaaagctg tggcttcgtg
     2401 gttgtaaccg atcaactgga gtgcatagac gatcagcgaa gaatctcgta accaggtgta
     2461 gcggtaatcc cagtttctaa cgccgccagg cgtttcaggt aatgaggttg ttggtgcagc
     2521 aatgatcgct cccgtcggcg caaaggtcag aagcttcaaa acgagggcgc tgcgatgaac
     2581 gaaggcagcg taatcaccct catacgtaca aagatccaac cactcgcgcc agtgggcaat
     2641 cgtctgctca aactcgattt catgatccgc ggctgccaac tcctgaaaat ctatttttga
     2701 gtcgcgagca taagagagat gtagccagac tcgatctccg tttgagattt taaatctaca
     2761 gcggagactg ctgtcaccgt ttgccttaag tgcaaaggga cagtagagca cgacggtttc
     2821 gtcagtacta cgagctgtac cgcctttatc aaaaggttcg attagagtct tggaacgagc
     2881 gtaattaaat gtcggatgga aatttacttc aacctcgacg tcgccgctta cgccttcaat
     2941 gagccgagaa atagaacgac ggagaatcgg cgggtattca atgtagcctg ccagacgacc
     3001 taccggcata aaatcactca atctgattct gccagagtca gtttcaaaga ctgtctgcaa
     3061 aacattagta ccttcaacat acctttggct cgagacgaaa ttcttgagcg gattgatagc
     3121 aaacgagcct ccgtgttccg cgtcgagaag cttgcagaag accgccggac tatcaaaatc
     3181 gggccaacag caccaatcga ttgagccatt ctttgagaca agtgcagcag tgtgaagatc
     3241 gccgatcagt ccaaagtctt tgattggcgg ataggttatt gttccactag gaatatcgga
     3301 tatgaccatt taacttccac tgcgaatcgc ctgctgcata gaaagcgcgg gagccagagc
     3361 cgggaggaat tttcgcgcgc gctgaaacgg ggccgagtca tgtctaacgg agcctggccg
     3421 cttaagaaga ttggatttct gcactattcc tttcgtaacg atcttcccgg cttgggatga
     3481 ttgctgtctc tcatttcagc gctcctattc cactgcattt ttgctagtta cggtgttcac
     3541 tgaaagagtg tgatcactga tccgaagtga gtgatcacac tcggacgtca atttgagtgt
     3601 tatcacttaa aggtctgagt gacaattcgg acgaaagact tctgaagcct tgttatcctc
     3661 aatgaatgca cgcgactatc gggcacaaac tcggcgattg acgtacacca ataaagagaa
     3721 aggagaagtt atgaaacaaa aacgagcaac ggaattaatc aacggggtcg ataccggacg
     3781 actttttgaa accgtagagt caattcgcaa atcacccgat ctggccgtgt tccgttttcg
     3841 attgaataac aattggctcg gtggcggtcg taataaatca atgatccaaa acttctacgg
     3901 cgctggtgag gaaaattcca cgcgcaaaaa gccattcgtc atcgatgctg atgagccggc
     3961 agttttgttg ggttcggata aggcccctaa cccggtggag tatctgctcc acgcgctcgc
     4021 tgcgtgcgta acaacttcca tggtctatca cgcggcggca aagggtatcc gaatcactga
     4081 ggtggaatcc cgtgttgagg gcaagttgga tcttagagga ttcctgggtc tgagcgaaga
     4141 tgttccgccc ggctacgaaa atattcaaat gcttttcaag atcaaggctg atgtgcccga
     4201 tgaccag
//
LOCUS       DASUTG010000278         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1081958, whole genome shotgun sequence.
ACCESSION   DASUTG010000278 DASUTG010000000
VERSION     DASUTG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081958"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF089_06315"
     CDS             complement(<1..658)
                     /locus_tag="VF089_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6173610.1"
                     /translation="MKILCRDLNADKDGIPGYIAHPERKEPGPAILIVHHHYGVTGHL
                     KSVTCDFAKLGYTTVALGIYKLLGEPDGVHEAQKKTTDGQFVEVIQRGWRYLTRRNDV
                     APKRCAVVGYCMGGRIGIHFVAATPDVRSFVGYYPSVRDEGPSQLRPRHPNDAVKDFK
                     CPSLIFFGGNDHVASVAVQDRLKTSFHANEQPLDWHFFQHAGHGFALGDGDCYDPRLA
                     G"
     gene            complement(1011..1859)
                     /locus_tag="VF089_06320"
     CDS             complement(1011..1859)
                     /locus_tag="VF089_06320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6173611.1"
                     /translation="MKRAGVKAAMGPLLLLFAWIIFPGPSAAAELKIAAKAAALMNAQ
                     TSKILWAQNQDLRLPPASTVKILTALVVLDHGRSGDVVTIPAEALRVSGATTQLRAGE
                     KLSVSDLLHAMLLGSGNDAAIALALHTTGSIDTFVEQMNQKAHKLEAIRSRFFNPTGM
                     PHANQLTTAQDLALITKAALENSEVRRIVGKRTYRWRSKGWQGTIKNSNKLLSSYSGA
                     IGVKTGNTREAGYCLVAAAQRGGQSYIAVVLKSEQKAVWQDAMNLLDYGFKHESLEYG
                     PASSDR"
     gene            complement(1915..2976)
                     /locus_tag="VF089_06325"
     CDS             complement(1915..2976)
                     /locus_tag="VF089_06325"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173612.1"
                     /translation="MHRLSKKFGANEILKDISFEVCDGEFCILLGPSGCGKSTILRLI
                     AGLEKPSSGEILFDGERIEHLPPRERNIAFVFQSYALYPHMTVFDNLAFSLKLRGEPA
                     AEIAAKVRDAARLLEIENLIDRRPHSLSGGQRQRVALGRAIVRQPQIFLFDEPLSNLD
                     AALRATMRVELARLHRRLGTTIVYVTHDQAEALTLGEKIIVLHQGTIQQIGSPADIYQ
                     RPANTQVGSFVGSPQMNLLRGTVDESGSVFHCGSLRLNLAATFARSLPQLIGNSVVIG
                     IRPEDLQLAAAGQSWIRGDVEIIEDLGSDRFLHVKCDSAELIARTGRESGVRQGDVVG
                     FSVAPEQVHIFKDDKRFDP"
     gene            complement(3221..>3542)
                     /locus_tag="VF089_06330"
     CDS             complement(3221..>3542)
                     /locus_tag="VF089_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6173613.1"
                     /translation="AALIDGCSRLQVLRRVVLPLAAPGILTAALLVFIQTWNEFFFAL
                     IIMTDPNVQTLPVGIALFPGEYTMPWGEIAAASTLATLPLIFLTLFFQRGIVRGLSAG
                     AVKG"
BASE COUNT          752 a         1013 c         1005 g          772 t
ORIGIN      
        1 gcccggccag ccgcggatca tagcagtcgc catcgcccag agcgaagccg tgaccggcgt
       61 gctggaagaa atgccaatcc agcggctgtt cgttagcgtg gaagctggtt ttcagacgat
      121 cctgcacggc gacgctggct acatgatcgt tgccgccaaa gaagatcagc gacgggcact
      181 taaaatcttt caccgcgtcg ttcgggtgac gggggcgtaa ttggctcgga ccttcatcgc
      241 gcaccgaagg gtaataacct acgaagctgc gtacgtccgg agttgccgcg acgaaatgaa
      301 tgccgatgcg cccgcccatg cagtagccga ccacggcgca gcgtttaggg gcgacatcgt
      361 ttcttctggt cagataacgc cagcctcgct gaatcacctc aacgaattgt ccgtcggtgg
      421 tttttttctg cgcctcgtga accccgtctg gttcgccgag cagcttatag atgccgagag
      481 cgacggtagt atatcccagc ttggcaaaat cgcaggttac cgacttcaga tggccggtga
      541 cgccgtagtg atgatgcacg attaaaattg ccgggccggg ttctttgcgt tcaggatggg
      601 caatgtaccc cgggatacca tctttatcgg cgtttaaatc acggcagagt atcttcatcg
      661 gcgcgcttct ccttgagcag tttttcgatc ttttctctgg tatcacgact ggctggtagt
      721 tatcatttac tttgaacccg gatcccgcag tttaagagta ataccgaaat caagtggcga
      781 ggcctccgga gcgaatgcaa tgggcctgtg gctgatatcg ggctgcgacg ccactgctgc
      841 agtggactcc cgggaaagct gccagccctg ggccaagcac catagacgga attgacattg
      901 ttcgttcctg tgccttcgct cctccagcct cactacttga ttggtacaca gccgtcatct
      961 ctaatacggc atcgtggctg aagtctcata acgaagcgga cgcaacgacg ctaacgatcc
     1021 gatgacgcag ggccgtattc gagcgactca tgcttaaacc cataatcgag aagattcatg
     1081 gcatcctgcc aaacggcttt ttgttcgctt ttcagaacga cagctatgta actttggccg
     1141 ccccgttggg cggctgccac aaggcagtag cccgcttctc gggtgttgcc ggtcttgacg
     1201 ccgatggcgc cactatagct ggatagcaat ttgttggaat tcttgattgt cccttgccag
     1261 cctttgctcc tccaccggta agttcttttg ccgacgatcc tacggacctc cgaattctcc
     1321 agagcggctt tggtgatcag cgccagatcc tgcgccgtgg tcagctggtt cgcgtgaggc
     1381 atgccggttg gattgaaaaa acgggaacga attgcttcca acttatgcgc cttttgattc
     1441 atctgttcaa cgaaagtgtc gattgagccc gtagtatgaa gcgccagcgc aatggcggcg
     1501 tcgttgccgg agcccaacag catggcatgg agcaaatcgc tgaccgatag tttttctccg
     1561 gctctaagtt gggtggtcgc gccggaaacg cgcagcgcct cggccggaat ggtgacaacg
     1621 tcgccgctac ggccatgatc gagcacgacc agtgcggtga ggattttaac ggtgcttgcc
     1681 ggcggcagcc gcagatcttg attttgcgcc cagagaatct ttgacgtttg cgcgttcatc
     1741 agcgcagccg ctttggctgc gatcttcaac tcggctgcgg cgctaggtcc tggaaaaata
     1801 atccacgcga agagcaacag gagggggccc atcgctgctt tgacgccggc gcgcttcatc
     1861 aactgaaact ttcttgtttg tttggctgag gagcgcgtgc ctgaagctga agggtcatgg
     1921 gtcgaatcgc ttgtcatcct tgaagatgtg cacctgctcc ggtgcgacgc tgaagccgac
     1981 gacgtctcct tgtctaaccc ccgactctcg gccggtacga gcgatcagtt cggcactgtc
     2041 gcatttcacg tgaaggaagc gatccgagcc gagatcctcg atgatctcga catcaccgcg
     2101 gatccagctc tgtccagccg ccgccagctg cagatcctca ggacgaatac cgataacaac
     2161 ggagttgccg attaattgcg gcagggatct cgcgaaagtc gctgcgaggt tcaggcgcag
     2221 gctgccgcag tgaaagactg atccgctttc atcgacagtg ccgcgtaaca gattcatctg
     2281 tgggctaccg acaaacgagc caacctgggt gttagccggt cgctggtaaa tgtctgcggg
     2341 cgatccaatc tgctggatcg tcccttgatg tagaacgata attttttcgc cgagggtgag
     2401 cgcttcggcc tggtcgtgtg tgacgtagac aatcgtggtg ccaaggcgcc ggtgcaaccg
     2461 cgccagctcc acgcgcatgg tcgcgcgcag cgcagcatcg agattcgaca gcggctcgtc
     2521 gaatagaaag atctgcggct ggcgcacaat cgcgcgcccc agcgcgactc tttgccgttg
     2581 cccaccgctc aggctatggg gccggcgatc gatcaaattt tcgatctcaa gcaggcgcgc
     2641 ggcatctcta accttcgcgg cgatctccgc ggcaggctcg ccccggagct tcagagaaaa
     2701 agccagattg tcgaatacgg tcatgtgcgg ataaagcgca tagctctgga agacgaaggc
     2761 gatgttgcgc tcccgcggcg gcagatgctc gatacgttcg ccgtcgaaaa gaatctctcc
     2821 ggagctgggc ttttccaacc cggcgatcag ccgcaggatg gtagatttac cgcagcctga
     2881 aggtccgagc aggatgcaaa actcgccgtc gcaaacctca aaggaaatgt ccttcaggat
     2941 ttcattggcg ccgaacttct tcgagagccg atgcacggtt agagctgcga tagggattcc
     3001 tccggttgtg gaactctgcg agcgcgaagg cgatgatccc tccagccgtc ggcaatagga
     3061 cactgcctcg agggccaccc cgcaatcccc ctcagggcga tgttccctat gttgggcctg
     3121 ccatcaacct gtatgcccag gtgcccacga cttccggtct caccaccttc gctcaccaag
     3181 tcagccgcag gttttgtcga atcaggatac attcttgctt tcaacctttt actgcgcccg
     3241 ccgacaagcc tctgacgatg ccgcgctgaa aaaagagggt cagaaaaatg agcggcaaag
     3301 tcgccaatgt cgaagccgcg gcgatttctc cccagggcat ggtgtattcg ccggggaaaa
     3361 gcgcgatgcc caccggcaga gtctggacgt taggatctgt catgatgatc aaagcaaaga
     3421 aaaactcgtt ccaggtctgg ataaatacta acaaagccgc tgtcagaatt cccggcgcgg
     3481 ccagaggcaa aacaactctg cgcagaacct gcaaccggct gcaaccatcg attagcgctg
     3541 cc
//
LOCUS       DASUTG010000279         4736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1675170, whole genome shotgun sequence.
ACCESSION   DASUTG010000279 DASUTG010000000
VERSION     DASUTG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675170"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1145)
                     /locus_tag="VF089_06335"
     CDS             complement(78..1145)
                     /locus_tag="VF089_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173614.1"
                     /translation="MIVDADAHVVETEHTWDYLDASEKKFRPQLFQSADNPNAQYWFV
                     DGKSIGFRPPTLTEQELIALSKSTGRELKTDPQARELRDVELRLKHMDKLGIDVQILF
                     NTMWIARVADKAEAEIALCKSWNHWMADVWKQGKNRLRWSCVVPAMTINQAIQQMRFA
                     KENGAVAVSLRPFEDDRHLVDPYFYPIYEEATRLDFAVAVHIANASPQLLDQFKPRYD
                     KMGGFAQFRIPTVITCLSLMMSEVPQQFPKLRWAFVEASAQWVPWVVKEAIRRTGTKG
                     YPDLPFKEFRIYVTTETNDDFDFVLRYAGEDNLVIGTDYGHTDASSEVDAIDIFRAND
                     SLPETIKKKILDDNPRALYTL"
     gene            complement(1202..2209)
                     /locus_tag="VF089_06340"
     CDS             complement(1202..2209)
                     /locus_tag="VF089_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173615.1"
                     /translation="MKILSLVALMLMTTEIAVAQDSFFKGKTVRIVVPFAAGGGYDIY
                     SRIIGRHMGKYIPGNPVFVIENMTGAGGLIGMNHVYKAAKPDGLTIGTPIGTMFIDQL
                     IGKPGIEFDGRKFEYIGAPAQDTQLLIVHKRTGIKTVEQWLSHPSPVKFGSTGPGSAN
                     ESIPKIVKEALNLPLQVVSGYKGSSVIRLAFTGGEVDGVANAWESMVSTWPQELESGD
                     AVILLQASGKRHPDLLNVPLVMDYAKTDMAKKLVRGVINNFGATARPYVVTPGTPRAR
                     VEVLRKAFMDTMKDPAFGTELKKAKLDLNPLDGATLENNIKELFALDQALIPKLTEIL
                     K"
     gene            2585..3412
                     /locus_tag="VF089_06345"
     CDS             2585..3412
                     /locus_tag="VF089_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6173616.1"
                     /translation="MPYAQAGEVRLYYESFGRGVPFLFVSGTGWPGEPWKLKQVPAFI
                     DRYQVIVYDHRGVGKSDAPAGYYSTRQFAQDAANLLDSLGISEPAHIIGHSMGGRVCQ
                     WLAIDYPHKVRSMIQAASGSGSMGNPDYPRWLTVNSLKSMIEKGYKEHMLSHFKSRFF
                     FPPEFVKADPEVLERLFKSFWDNRPQLDAYLRHVIARNQHETGELLDKIDAPTLVLVG
                     GEDKADADTGNHVITSEHLRDNIRNAEFAVVEGCGHGFFWQKPEETNKIIRNWLDRH"
     gene            3644..>4736
                     /locus_tag="VF089_06350"
     CDS             3644..>4736
                     /locus_tag="VF089_06350"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6173617.1"
                     /translation="MFDSSKIQPQIAFTDLREWMREAEKLGELKTVLGASWQEDIGLA
                     TDVVVPPDDGPAVIFDEVPGCPKGFRILINAFAGKRRAMTFGFPQGLSKQELSQAYFD
                     HFQKAPNHMPSVFVKDGPIFENVVNGADVDIMKFPTPVWHVNDGGRYIGTGCYSVTMD
                     PDEKWVNVGCYRAMIQDAKTVSLLMVPGKHGYMHREKYFKRGQKMPLALVIGGDPLFF
                     FMAGTEHPYGLCEYDIVGGMRGKPVEVVRGPLTGLPFPAGAEIVFEGYLNNDNRKNEG
                     PFGEWTGYYASDESAQPVLEIEAVYHRNDPIILGVPPIGGGSDEMARYRAIMRSAMLK
                     QQLQSAGVPDVRQVWAHEIGASRMLIALAI"
BASE COUNT         1059 a         1203 c         1324 g         1150 t
ORIGIN      
        1 cgcgataaag ctgcgtagat ccttgggcat cgatcggtgt ggttaatcgc gatggccgct
       61 gcggaccttc gaaagaccta caacgtgtat agcgcgcgtg gattatcgtc gagaatcttc
      121 ttcttaatcg tttccggcag cgaatcgttt gcccggaaaa tatcgatggc atccacttcg
      181 ctcgatgcgt cggtatggcc gtaatccgtg cctatgacga ggttgtcttc gccggcgtag
      241 cgcagcacga agtcgaagtc atcattcgtc tctgtggtga cgtagatccg gaactctttg
      301 aaaggcaggt cgggatatcc tttggtgccg gtccgccgaa tcgcttcctt aaccacccat
      361 ggcacccatt gagccgacgc ttcaacaaaa gcccaacgga gtttcggaaa ttgctgcggc
      421 acttcgctca tcatcaaaga caaacaggtg attaccgtag gtatacgaaa ctgcgcgaag
      481 ccgcccattt tgtcataacg cggtttgaac tgatccagaa gctgcgggct cgcattggca
      541 atatggacgg cgacagcgaa atccaagcgg gtcgcttcct cgtagatcgg atagaagtag
      601 gggtcgacca ggtgcctgtc gtcttcaaac gggcgcaggc tcaccgcaac ggcgccgttc
      661 tctttggcaa agcgcatctg ctggatcgcc tggttgatgg tcatcgcggg gacgacgcac
      721 gaccaacgca agcgattttt gccttgcttc cagacatcgg ccatccagtg attccaggat
      781 ttgcaaagtg caatctctgc ttcagccttg tcggcgaccc gggcgatcca catggtgttg
      841 aataggatct ggacatcgat gccgagcttg tccatgtgct ttaagcgcag ctcgacatcc
      901 cggagctcac gcgcctgcgg gtcggtcttg agctcgcggc cggtcgactt tgaaagcgcg
      961 atcagctcct gttcggtcag ggtggggggg cgaaagccga tcgatttgcc gtcgacgaac
     1021 caatactgcg cattgggatt gtcggctgat tgaaatagtt gcggccggaa tttcttttcc
     1081 gaagcatcga ggtaatccca agtgtgttcg gtctcgacca catgggcatc agcatcgaca
     1141 atcatggttc agctccttct gcgatgaatc caacgggtag aaacccgatc tcgatccttt
     1201 cttatttcag gatctctgtc aacttgggga tgagcgcttg atcgagtgcg aacagctctt
     1261 tgatgttgtt ttccaaagtt gcgccgtcga gcggatttag atccaatttt gctttcttaa
     1321 gctcagtccc aaacgccgga tctttcatgg tatccatgaa agccttgcgc aaaacttcca
     1381 cacgcgctct cggtgtaccc ggcgtcacga cataaggccg tgccgttgcg ccgaaattat
     1441 tgatcacacc gcgcaccagc tttttagcca tatccgtttt cgcatagtcc ataaccaatg
     1501 gaacgttgag taaatcaggg tggcgttttc cggacgcctg caacaaaatg actgcgtcgc
     1561 cgctctcaag ctcttggggc caggtcgaaa ccatcgattc ccaggcattg gcgacaccgt
     1621 cgacctcacc gccggtaaag gccagccgga tcaccgagct gcctttatag ccagaaacaa
     1681 cttgcagcgg caggtttagc gcttctttga caatcttcgg aatcgattcg ttggctgagc
     1741 ccggcccggt gctgccgaat tttaccggag aagggtgcga tagccattgt tcgaccgttt
     1801 tgatgccggt gcgtttgtgt actatcagca gctgggtatc ctgcgccggc gcgccaatat
     1861 attcaaattt gcggccgtcg aattctatgc ccggtttgcc gatgagttga tcgatgaaca
     1921 tggtgccgat gggggtgccg atggtgaggc cgtcgggttt ggccgcttta tagacgtgat
     1981 tcatgccgat taggccgccg gctccggtca tgttttcaat cacgaagacc ggattgcctg
     2041 gaatgtactt acccatgtgc cggccgataa ttcgggaata gatatcgtag ccgcccccgg
     2101 cagcgaaggg caccacgatc cgcacggtct tgcctttgaa aaatgagtct tgggcgacgg
     2161 cgatctcagt ggtcatcagc atcaacgcaa ctaggcttaa aattttcatt ctactgccct
     2221 cttctcatcg cacggcaaag agcactggct cagtttgaac ttggagtcct attcgaagat
     2281 tctcgttcag tttctcatga gtctctctga atgatgcgta ccgtcatcaa aatacgggag
     2341 gtgtcatctc gaacccgcgg aatgctcagg ctaaactccg tgagggacgc ttatggacgt
     2401 aatgctaccg atccgatatt tagctggaga ttagccgatt ttgactggtt acatcaacaa
     2461 caatcgcgta agtaaagcca gtacattcca tggattctgg cagtgaaatc gcaattgcgt
     2521 atcaggggtt tactttctga agctgcatag cccattcatc agaaatacgt ccaggaggtt
     2581 ttagatgccg tacgcgcaag caggtgaggt tcgtttgtat tatgaaagct tcggacgcgg
     2641 agtgccgttc cttttcgtct ccggcacggg ttggccaggt gagccgtgga agcttaaaca
     2701 agtgccggcg ttcatagatc gatatcaggt aatcgtgtat gaccatcgtg gagtgggaaa
     2761 gtcggatgcg cctgcgggat actattcgac ccggcaattt gctcaagatg cggctaactt
     2821 gctcgatagc cttggcatca gcgagccggc tcatatcatc ggtcactcaa tgggtgggcg
     2881 agtgtgccag tggcttgcca tcgattaccc gcacaaggtg cgcagcatga ttcaagcggc
     2941 gtccggctca ggttccatgg gcaaccccga ttacccgcgc tggctcactg tcaatagtct
     3001 caagagcatg atcgagaagg gttataagga acacatgttg agtcacttca agagccgttt
     3061 tttctttccg ccggagttcg taaaagcgga tccggaagta ttggaaagat tatttaaaag
     3121 tttctgggac aatcggccgc agctcgatgc ctatttacgc catgtgattg cccgtaacca
     3181 gcatgagacc ggtgagctac tggacaagat tgatgcgccg actctggttt tagtgggcgg
     3241 cgaagataaa gccgatgccg ataccggcaa tcatgtgatc acgtcggagc atcttcgcga
     3301 taatattcgt aacgccgaat tcgccgtggt ggagggctgc ggccacggtt ttttctggca
     3361 gaagccggag gagactaaca agatcatcag gaattggctc gacaggcact gatgcgcctg
     3421 ctccacgtgt gcacgttggt atgaaattat atccagaatc tgcacgtttg ttcgtgctcc
     3481 gatccatcgt cgggaacgaa gcagtaacag ctgcagatta tgaagtcaca tgccgcgtgc
     3541 ttcaattgaa tatgacatgc ccaactatcc gccgcagagc cggttccatt gcgtgcgaga
     3601 accatgttag attgacggtg atttaatcgt gtaaggagcg actatgttcg acagctcaaa
     3661 gattcagccg cagattgcat ttaccgacct acgcgaatgg atgcgcgaag ctgaaaagct
     3721 cggagagttg aaaaccgtcc ttggcgcgtc ctggcaggaa gacatcggct tggccacgga
     3781 tgtggttgtt ccaccggatg atgggcctgc ggtgattttc gatgaggtgc ccggctgtcc
     3841 caaaggattt cgcatattga tcaacgcttt tgccggcaaa cgtcgcgcca tgacctttgg
     3901 ctttcctcag ggattgtcca aacaggaatt gagccaagcc tatttcgatc attttcagaa
     3961 agctcccaat catatgccgt cggtatttgt aaaagatggg ccaatttttg aaaacgttgt
     4021 caatggcgct gatgtcgata tcatgaagtt ccctacgccg gtgtggcatg taaacgacgg
     4081 cggccggtat atcgggacag gttgttattc agtgaccatg gacccggacg aaaagtgggt
     4141 taacgttggt tgctaccgtg caatgatcca ggacgcaaaa accgtcagcc tgctgatggt
     4201 gccaggcaaa cacggctaca tgcatcgcga gaaatatttc aagcgcggtc agaagatgcc
     4261 gttggcgttg gttattggcg gcgatccgct gtttttcttt atggcgggaa ccgagcatcc
     4321 gtacggcctc tgtgaatatg acatcgtcgg cggaatgaga ggcaaaccgg tagaggtggt
     4381 gcgcgggccg ctcactggtt tgccgtttcc ggccggtgcc gaaattgtgt tcgaaggata
     4441 cttaaacaac gataatcgca agaatgaggg gccgttcggc gaatggaccg gctattatgc
     4501 atcggatgaa agcgcgcagc cggtgctgga gatcgaggcg gtttatcatc gtaacgatcc
     4561 gatcattctc ggagtaccgc cgatcggcgg cggctccgac gagatggcgc gttaccgcgc
     4621 gattatgcgc tcggccatgc ttaaacaaca actgcagagc gccggggttc ccgacgtacg
     4681 gcaggtatgg gctcatgaaa tcggcgcgtc tcgaatgctg atcgcgctgg ccattc
//
LOCUS       DASUTG010000280         5723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2121010, whole genome shotgun sequence.
ACCESSION   DASUTG010000280 DASUTG010000000
VERSION     DASUTG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121010"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1102..2118
                     /locus_tag="VF089_06355"
     CDS             1102..2118
                     /locus_tag="VF089_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173618.1"
                     /translation="MGKRQPATISLSGLCLLSIGFFMFLAAAASEGATLSRLRVGYPS
                     PSASFFPLFATKEAGLLEKYGFDTEMVYVQGVQLVQVHVAGQLDFSTISSVVYLQASV
                     EGADLIQVASSIDNQLMKLMVHPSIAKPEDLRGKTLAVTRFGSLTDLLIRPVLKNWGL
                     EPQKDVKLIQIGRMPDIATAIAQKSVDGGVISFPTSVQAEKMNIRTMLDFAESGFELP
                     ATTVVVSRRYSKSNRDAVLRFLKAYIEGTRRLFIDRELGIRALRRYGGISDRDMLSTT
                     YDLFTSRYIKKIPTVNAKGVENSLNLIAESNPRAKNRKAEEFMDTSFMEELESTGFIS
                     SVWK"
     gene            2277..3317
                     /locus_tag="VF089_06360"
     CDS             2277..3317
                     /locus_tag="VF089_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173619.1"
                     /translation="MFLRISAYLACLFLLSVTYTVNAQTSFYQGKIVRIVVGNLAGDT
                     HDLFARAYARSMGKYLPGNPEIIVQNMPGAGTMIAANHVYNIAKPDGLTLGSLSPALY
                     YAQLTGSKEVKFDWPKFTWIGSPERNGHVLFMRSDSPYKTLADIRDAKEPPRCSATGV
                     GTSGHDVPKLFEETLGLKFRIITGYPGGAEQDLAMERGEVHCRAITTAAFFAREPFKT
                     WYKAGFVRIMIQTSRQRNPKVSDVPTLFELMEQHKTPDIKRRQALVYLGAGGFGSWPI
                     LSSPGVPADRTAMLRDAYAKTLKDPDFLAEAKKNGWELRPVSGEELQALAKDVIDQPP
                     EVVDWLKKLLAK"
     gene            3341..4432
                     /locus_tag="VF089_06365"
     CDS             3341..4432
                     /locus_tag="VF089_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173620.1"
                     /translation="MWRNSKILTNSNTKGFHGLFSYLLTIIGLWILLDLQPGFAQAPF
                     YQGKTIRVIVGYQPGDSHDQWARTYVRFLGKHIPGNPEFVVQNMPGAGGMIAANHIYN
                     LTKPDGLTLGSFGGALFMAQITGRKEVQFDWPKFNWLMTPERGGHLVFMRTDIPHKTF
                     DDLRQTNDPPKCSATGVGSSGYDIPRLLEEALGFKFQVISGYPGGAEQDLAMERGEVH
                     CRAFTIDAFFGREPFPSWIKKGFIKVWLQTERKRNPKLPEVPTVFELMDQYKVSEPKR
                     RVVEAYLGIWGFGSRPIVTSPGVPADRVKILREAFAKMFKDPEFLDDIKKRRWEVSPV
                     SGEELQALANEVVDQPADVTAALKRILSK"
     gene            complement(4669..>5723)
                     /locus_tag="VF089_06370"
     CDS             complement(4669..>5723)
                     /locus_tag="VF089_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6173621.1"
                     /translation="LIRDIVKPELEQAGLRPLIDGIGNLILHIKGKTRNDRLMLVGYA
                     MNAAPSTMPNPYSGEIVDGAPYKLHGECVWGRGACEQKGSLAAMIAAIRFLGASKIEL
                     PSDLYLVVSTAGETGRHDALAYVLDHGRIETDWCIIDGPPEIQLGNKGRIDVLVIVRG
                     KQSHSSRPWEGVNAIEGAMKVLDKLKPLMPYPEAKTHASLGNVSLAVNSIESFPKATH
                     TIQSECRITFDRRLLPGDDPKRAVQQMIDAIGKIEPYQIEVQPKDFMHPSELGKDADL
                     VRALEHGIRTVLNYEPQYTFSSAANDTGLFNFRGIQAINYGSRDIRFQHTDHDLVSVN
                     DVFNAAKVFAFVALHR"
BASE COUNT         1373 a         1488 c         1527 g         1335 t
ORIGIN      
        1 tcatacccag agcttacaaa gaatcgtctt cgctgtgttt catcacttga atactaactg
       61 gagtctaaac cccgtcaaag attttacaca caactcttga cgctacctag atctgtgtgt
      121 tggagcaaca aaaacgcttt cgaccgtttt gctggagccc accgaagacc caactttgac
      181 cgctagtttg cgagtaccgt taccgttctt ccagctgaag ggaacggaga acggtaaccg
      241 ctgcaatgcg cccacaagaa tccgcaaaac acagctcatt cccgtacggt gcgtgagttc
      301 cacgggactc atagtgggag cttcgcgatt ccgtaacagt aaaggaggca aggagagccg
      361 aacttcgaac actcgaagaa cacgcgcgcc taagtgtggt gagttttcgc agttgcgcgg
      421 ggaactctag atctgcgatc ggcttctgga aaaaccagca tatccataat ccaaacacat
      481 ggccctgatg tacggaaacg cactaattac aatgattgct gttcctgctc ttgccgggtt
      541 ttcctggatc gctctcacga cctcgacgcc ctccatatca gacaatcgag ctcgaggagt
      601 acggcatccg gaacatgttc tttcgtagcc ttgatggcgc cggcgcccct ccgagcaagt
      661 aagctggcat aaccggctat agacaaatgt tttgtaaggt aggcaatttc cttcgcctgc
      721 ttgccgacaa cgagaactgt ttttgttttt tcggacgcca cgttcatttt cggccgctaa
      781 gttacggcaa caaacatgac ctgcccccga atcacgaact gctctgcgca tcaccttgat
      841 aattaactct gcgggatgca agcagcgcta gatggccaca ttgaacaacc gcgagttggc
      901 cttcagttcc caacgatgag tacacgtccg cggctgagaa ctccgtgacc aagcagtcgt
      961 attgacgact tcattatctt gacattgcga actagccgca ctaaggctgc ggagaggaat
     1021 cggaaggact tcaatctcgg atgatcggcc gcgattatcc gcaacacaca tgatggaaat
     1081 ctgctcatcg tggagaaata tatgggtaaa cgacagccag ctacgatatc attatccggc
     1141 ctctgtcttc tgtcgattgg gttcttcatg tttctggccg cagcggctag cgaaggcgca
     1201 actctatcgc gcttgagggt cggctatccg tcacccagcg ctagcttctt ccctcttttt
     1261 gccacgaagg aagccgggct gctagaaaaa tatggatttg ataccgagat ggtctatgtt
     1321 cagggagtac aactggtaca ggtccatgtt gccggccagc tcgatttttc gacgatttct
     1381 tccgtggttt atcttcaggc ctcggtcgag ggtgccgatc tgattcaggt ggcaagctcc
     1441 atcgacaacc agttgatgaa gctcatggtc catccgtcta tagcaaaacc ggaggatctc
     1501 aggggaaaga ccctcgcggt gacgcgattc gggtccctca cggaccttct gattcgtcca
     1561 gtgctgaaga attggggact cgaaccgcag aaggacgtga agctgattca gatcggtaga
     1621 atgccggata tagcgacagc gatcgctcag aaatccgtcg acgggggcgt catctctttt
     1681 ccgacgtcgg ttcaagcgga gaagatgaat ataaggacca tgctggattt tgccgagtcc
     1741 ggtttcgagt tgccggcaac tacagtggtg gtgagccggc gttatagcaa gagcaatcgc
     1801 gatgcagtgt tgagatttct caaggcttat atcgaaggga cacggcggct ttttatagac
     1861 cgagagctgg gcattcgggc tctgcggcgg tatggcggca ttagcgatcg cgatatgctg
     1921 tcgacgactt acgatctttt tacttcccgg tacatcaaga aaattcccac agtaaatgcc
     1981 aaaggagttg aaaactcatt aaacttgatt gcggagagca accccagagc aaaaaaccgc
     2041 aaagccgagg agtttatgga caccagcttt atggaggaac tggagagcac cgggtttatc
     2101 agctcggttt ggaagtgacc gaaatatggt cactctcaga ctatgttgaa aatagaatct
     2161 tcctggcgag ccgtgcaacg taggatcgag tttggagcaa acacagaaaa gtgaaagcag
     2221 atcctttact tcaccgctaa agcaagggta atagagaccg gtaaggagat ccgtcgatgt
     2281 ttctcagaat atcagcctat ctggcttgcc tatttctcct gtcagtcacg tatacggtta
     2341 acgcgcagac ttctttctat caaggaaaaa tcgtccgtat tgtcgtcggc aatttagcgg
     2401 gcgataccca cgaccttttc gcgcgcgcat atgcgcgcag tatgggcaaa taccttccgg
     2461 gaaatccgga aatcatcgtg cagaacatgc ccggcgcggg cacgatgatc gccgccaatc
     2521 atgtatacaa tattgccaag cccgatggtt taacgctggg atcactctca ccagctcttt
     2581 attacgcgca gctcaccggc agcaaggaag ttaagttcga ttggccgaag tttacctgga
     2641 tcggttcacc ggaacgcaac ggccacgtat tattcatgcg ctcggattct ccttacaaga
     2701 cgctcgcaga tattcgtgat gccaaagaac cgccgcgctg ctcggccacc ggagtcggaa
     2761 ccagcggcca tgacgtacct aagttgtttg aggaaactct tggtttgaaa tttcggatca
     2821 tcaccggcta tcccggcggc gccgagcaag atctcgccat ggaaagaggc gaggtgcact
     2881 gccgggcgat tacaaccgcg gcattttttg cgcgcgagcc gtttaaaact tggtataaag
     2941 caggatttgt tcggatcatg atccaaacct cgcggcaacg aaatcccaaa gtctcggatg
     3001 tgcctacgct gtttgagctg atggagcaac ataaaactcc ggatatcaag cggcgccaag
     3061 cgctggttta tctaggcgca ggcggatttg gctcctggcc gattctttcg agtcctggag
     3121 tgccggcaga tcgcacagcg atgctacgcg atgcgtacgc taagacactt aaggatccag
     3181 atttcttggc cgaagcgaag aaaaacggct gggagttaag acccgtcagc ggcgaagagc
     3241 tgcaagcctt ggccaaggat gttatcgacc agccgccgga ggtcgtcgac tggttaaaaa
     3301 aattattggc aaagtaattt tggaaatatg aggttgcgct atgtggagaa acagcaagat
     3361 tttgaccaat tccaacacga aggggtttca tggcctcttt agctatcttc tgacgattat
     3421 cggcttgtgg attttgttag acctccaacc gggatttgct caggctcctt tttaccaggg
     3481 caaaaccatt cgggtaatcg tcggttatca gccaggcgat agtcatgatc agtgggcgcg
     3541 gacctatgtc cgcttcctag gcaagcatat tccgggaaat cccgagttcg tcgtacaaaa
     3601 catgccgggc gccgggggga tgattgccgc caatcatatt tacaatttga ccaagcccga
     3661 tggtctaacg ctgggctcct ttggcggggc actttttatg gcgcagatca ccggccgtaa
     3721 agaagtccag ttcgattggc ccaagtttaa ttggctcatg acgcccgagc gcggcggaca
     3781 tttagtgttc atgcgcaccg acattccgca caaaaccttc gatgatcttc gacaaaccaa
     3841 tgatccaccg aaatgttcgg ccaccggcgt cggcagctct ggctatgata tccctaggct
     3901 gctcgaggaa gccctgggat tcaaatttca ggtcatcagc ggttacccag gcggtgctga
     3961 gcaagatctg gcaatggaac gcggcgaggt tcactgtcgc gcctttacaa tcgacgcctt
     4021 cttcggccgg gaaccgtttc caagctggat taaaaaagga tttatcaagg tttggcttca
     4081 aaccgagcgc aaacgcaatc ccaagctgcc cgaggtcccg acggtattcg aactgatgga
     4141 tcagtataag gtcagcgagc ctaagcggcg tgtagtggaa gcgtacctag gcatctgggg
     4201 tttcggctca cgccccatcg ttacctcgcc gggtgttccg gcggaccggg taaagattct
     4261 tcgcgaggct ttcgccaaaa tgttcaaaga cccggagttc ctcgatgata taaagaagag
     4321 aagatgggag gtaagcccgg taagcggcga agagctacaa gccctagcca acgaagtcgt
     4381 tgatcagccg gccgatgtga cggcggcgct aaaaaggatc ttgagcaaat aactctctgg
     4441 ctagacgggt gtcgtgcgcc gcgcagtttt gcacccacct gatgaaaaat tgtcgctgga
     4501 agctcaatta gataaattcc ccaagctaca gaaaagcatg gattccgctc ggttgattcc
     4561 tttcgagtcg cggtcgtgag ggtttgacga ttatcgcaga ttttatcttt aactatgaac
     4621 ggctgaattc tggcggctaa tcagtgaacc cgattcaatg aacgccccct agcggtgcaa
     4681 tgccacaaaa gcaaaaacct tcgccgcatt gaacacgtcg ttgaccgaca ccaggtcatg
     4741 gtcggtatgc tggaagcgga tatcgcggga accatagttg atcgcctgaa tgccccggaa
     4801 attgaataag ccggtatcgt tggctgcgct ggaaaaggta tattgaggct cgtaatttaa
     4861 gactgtgcgg atgccgtgct cgagggcgcg caccagatcg gcgtccttgc cgagttcgct
     4921 cgggtgcatg aaatcctttg gctgaacctc tatctggtag ggctcgattt tgccgatggc
     4981 gtcaatcatc tgctgcacag ctcttttggg atcgtcgccc ggaagaagtc ggcgatcaaa
     5041 cgtgatgcgg cactcgctct ggatagtatg cgtagccttg ggaaaactct cgatcgaatt
     5101 gaccgcgaga ctcacgttgc ccagcgaagc gtgagttttg gcttccggat agggcatcaa
     5161 cggtttaagt ttatcgagca ccttcatcgc gccttcgatc gcgttgaccc cctcccacgg
     5221 ccggctactg tggctctgtt taccgcgcac aatcaccagc acgtcaatac ggcctttatt
     5281 gccaagctgg atttcgggcg ggccgtcgat gatgcaccag tccgtttcga ttcggccgtg
     5341 atccagaacg taagcaagcg catcgtgccg tccagtttcg ccggcggtgc tgacaaccag
     5401 atagagatcc gagggaagct cgattttgct ggcgccaaga aagcgaattg ccgcgatcat
     5461 cgcggcaagg cttcccttct gctcacacgc tccccggccc cacacgcact cgccatgcaa
     5521 tttgtagggc gcgccgtcga cgatctctcc tgaataagga ttcggcatcg tactgggagc
     5581 cgcattcatc gcataaccga ccagcataag acggtcgttt cgcgtctttc ctttgatgtg
     5641 caggatgaga tttcctatcc cgtcgatgag cggtcgcaaa cccgcttgtt ccagctcggg
     5701 cttgacgatg tcgcggatca gcg
//
LOCUS       DASUTG010000281         2853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_786221, whole genome shotgun sequence.
ACCESSION   DASUTG010000281 DASUTG010000000
VERSION     DASUTG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786221"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1289)
                     /locus_tag="VF089_06375"
     CDS             complement(204..1289)
                     /locus_tag="VF089_06375"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6173622.1"
                     /translation="MSDCRAAVITAHNQPLTIERVPIPDLEPGALLVKIAASTLCGTD
                     VHRWHGPLGDGDSLPIITGHEPCGYIEEINGERTDIFGKPVKRGDRIVWSYVACGSCY
                     YCSVALQPCICPGRASWGHNRSDQYPYLLGSCAEYMYVPPPCLIIKVPEEVSSASAAA
                     SACAYRTVMHGFDRLGAIKSNETVVIQGSGPLGNFATAVAKDHGAKKVLVIGAPAARL
                     EVTRRMGADAVLNLEEVTDARDRRQWVHDHTDGRGGDVVIQVANNMAVPEGLTLLRGG
                     GRYLNIGAGGKANIAVERLPQEMTLVTVRSAEPRHWLQAIDFLASRRDRFPFEAMITA
                     RYDLEHVNDALQAMASYQVVKAVIDLN"
     gene            complement(1444..2529)
                     /locus_tag="VF089_06380"
     CDS             complement(1444..2529)
                     /locus_tag="VF089_06380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173623.1"
                     /translation="MSSSLATRIIDGDGHIIEDHAGIIAHMESPYREIANRKGIVFPP
                     LDHLHSGRAVETPPQRDQRPNVGPEGWLDFLDDVGIDWTVLYPTTALAYGKIVSLDYA
                     VAAARAYNDWLCETYIKYNPRFKGMAIIPLQDPEEAAKELRRAVTELGMLGAMMPSNG
                     LPQPLGAKAYWPIYTEADRLGCCLAIHGGAHDRFGMDHMNMYVPVHALGHPWGLTFNC
                     ANIVYNGIFDRFPRVRIAFLEGGVAWLLLLLERLHASHETHFQYIPPSEFGIREEDDP
                     SKYIKKQIQDGRFFLGIETEELTLPFAIKIVGNSPFLYSSDFPHEVTHESCKHDLGEL
                     MESEEITAEDKAAMLYKNAESFYRLPL"
BASE COUNT          616 a          783 c          787 g          667 t
ORIGIN      
        1 agtcggctac tacttagagc ttcagattac tattgctctg gcataacgcg actgaattga
       61 ttgttgattt gcgcgtaagc atagttgttc acagtaaggc tcactagcgg catttgagga
      121 agtcgtcggc tgaaagcaaa atcggccgtt ttagatgaga ctccgtgcga tctctcgcct
      181 cagagtcgaa cttgagcccg atcttagttg aggtcgatta ccgcttttac cacctggtaa
      241 ctcgccattg cttgcagcgc gtcgtttacg tgttcgagat cgtagcgcgc ggtaatcatc
      301 gcctcgaacg gaaatcgatc ccgccgcgac gccagaaaat cgatggcttg taaccagtgg
      361 cgcggttccg cggatctgac ggttactaga gtcatctctt gcggcagacg ctcaaccgcg
      421 atgttggctt tgccacccgc gccgatgttc aagtagcgcc cgccgcctcg aagcagtgtc
      481 agtccttctg gcaccgccat gttgttggca acttgaatga caacgtcgcc gccacggccg
      541 tcggtgtgat cgtgcaccca ctggcggcgg tctcttgcgt ccgtgacctc ctccagattc
      601 agtaccgcat cggcgcccat gcgccgggtg acttccaaac gagccgccgg cgcgccaatc
      661 actaatactt ttttggcgcc atgatctttg gccactgcgg tagcgaagtt gcctagcggc
      721 ccgctgcctt gaatgaccac ggtctcgttg ctcttgatgg cgcccagccg gtcaaagcca
      781 tgcatcacgg tgcggtaggc acacgcggaa gccgcggctg aagcggatga aacctcctcg
      841 ggcactttga taattagaca aggaggcggc acatacatat actcggcgca gctacccagc
      901 aggtaaggat attgatcgct gcggttatgc ccccatgatg cgcgccctgg acagatgcaa
      961 ggttgcaaag cgacgctgca gtagtagcaa ctcccgcaag cgacatagct ccagacgatg
     1021 cgatcgccgc gctttaccgg cttgccgaaa atatcggtgc gttctccgtt gatttcctcg
     1081 atgtagccgc agggttcatg gccggtgatt atcggcaggc tgtccccgtc tccaaggggt
     1141 ccatgccagc ggtgcacgtc cgtgccgcat agtgtagagg ccgcgatttt gaccagcagc
     1201 gctcccggct caagatcagg aatcggcact cgctcaatcg tcagtggttg attatgggcg
     1261 gtgataacag cagcgcgaca gtcgctcatg aataataaac ctccagcctt gtacgggctg
     1321 attcgttaca agaggaagtc cctgcggcga acacattgtg aacttattgt tttcgccgac
     1381 tcagaacagt tccgcctcat atcaactgac ttcagctctc gcttcgctaa actctttatt
     1441 catctatagc ggcaatctgt agaagctttc ggcgttcttg tacagcattg cggctttatc
     1501 ttccgcggta atttcctcac tctccatcag ttcgccaaga tcatgcttac aactttcgtg
     1561 ggttacctca tgggggaaat cggaggagta gaggaacggc gagttgccga cgattttgat
     1621 ggcaaaaggg agagtgagtt cctcggtttc gatgccgagg aaaaagcgcc catcctggat
     1681 ttgttttttg atgtatttgc taggatcgtc ttcttcacgt atgccgaatt cggatggcgg
     1741 aatatactga aaatgggtct catgcgatgc gtgaaggcgc tccaacaata gcagcagcca
     1801 agccaccccg ccttcgagaa atgcgatccg cactcgtggg aatcggtcga agataccgtt
     1861 gtaaacaata ttggcgcagt tgaaggtcaa tccccacgga tgacctaacg catgaaccgg
     1921 cacatacatg ttcatgtgat ccatcccgaa acggtcgtgc gcgccgccat gaatcgcgag
     1981 acagcagccg agtcggtcag cttcagtgta aatcggccag tacgccttgg cgcctagagg
     2041 ctgcggcaaa ccgttggaag gcatcatggc gcctagcatc cccagctcag tcactgcccg
     2101 gcgcagctct ttggcggctt cttccgggtc ttgcaacgga atgatcgcca tgcccttaaa
     2161 gcgcgggttg tacttgatat aggtttcgca aagccagtcg ttataagcgc gagccgcggc
     2221 aacagcatag tcgaggctga cgatcttgcc gtaagccaga gccgtggtcg ggtaaagcac
     2281 cgtccagtca atcccgacat cgtccaggaa gtcgagccat ccctcgggtc cgacgttcgg
     2341 gcgctgatcg cgctgcggcg gtgtttccac ggcccggccg ctatggagat gatccagtgg
     2401 gggaaatacg atgcccttgc ggtttgcgat ttcccgatac ggagattcca tatgggcgat
     2461 aattcccgcg tggtcttcta taatatgacc gtcgccatca atgattctgg ttgccagtga
     2521 gctgctcatg cgtcctccta tcgattaaac aattagcgct gtcggaaggg cggcgcccaa
     2581 aaaccaacgg gctgttttgc gcaacgggtc cgcgctctcg aagcttatat ataaaccgga
     2641 gttaagatgc gtatcaagtt cgcgaccaga gttatgttaa actctgaggg ttgtgccgat
     2701 ctcgcgtgta tttactaagt tccccatcaa cggtggctat aacaagccgc ttgccaggtt
     2761 gtccaacaac tctgcgatat catcgaggga attcgacctg ggggagccgg ccaggtgagg
     2821 ggtaacgatg acgttgcgca atttaatcag cgg
//
LOCUS       DASUTG010000282         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_193731, whole genome shotgun sequence.
ACCESSION   DASUTG010000282 DASUTG010000000
VERSION     DASUTG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193731"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VF089_06385"
     CDS             <1..1167
                     /locus_tag="VF089_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6173624.1"
                     /translation="GGSRAANFSAAALLQLESLGILQQATALSSVSGSSLTAAYYGLF
                     SEDKVHHWNPQQVRQRLVKNFELDWFWRWFIPWNAVRYWFTGFDRSDIMADVFDHNLF
                     EDKTFGQMPKTTPKILINATSLTENGALFLFTDEAFNKIGSGLDKYAVSRAVMASGAF
                     PGAFHNVTLRDYTQPKQYVHLFDGGPSDNLGINSLRKVARELYLEPESARPKKCLVLI
                     VDAYTETWNLSREDSDTRKAITDFVIDTNALEASSVLLALRRHKLLEEMGIRPDGNTA
                     PDVYQSFRLFAEADDPELKKNPKLQDVECHAWLITFQRLQHLTHLPNAGTISAIVNNI
                     PTRYTLLAPHGLGAAEAQEALFTAAEMLIKEDKETLAAICKDFADNFAEMKCPK"
     gene            complement(1592..>2206)
                     /locus_tag="VF089_06390"
     CDS             complement(1592..>2206)
                     /locus_tag="VF089_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6173625.1"
                     /translation="EWAEDLARSQTPMHALFVHKTLKTILRADDCLVFDGGDFCHFGR
                     SFLPALKPKRWFNVSSLGMLGSSLPTALAAKIAYPDSRVFMLSGDGAFGFNAMEFDTA
                     VRHKLNIVALLGNDAAWGIDRQIQLGLYGRAVATDLLQTRYDQVVQGLGGYGELVERP
                     EDLEPGLKRALAAGKPALLNVAVQRAISPRAEAAIGRRKVAAQQ"
BASE COUNT          510 a          597 c          595 g          504 t
ORIGIN      
        1 ggcggcagtc gtgcggcgaa tttttctgcc gctgcgctac ttcaactgga gagtttagga
       61 atactgcagc aagcgactgc tctttcgtcg gtctcaggca gttccttgac cgccgcctat
      121 tacggccttt tcagtgaaga caaggttcat cattggaatc cacaacaagt ccggcaacga
      181 ttagtgaaaa atttcgagct cgactggttt tggcgatggt ttataccctg gaacgcggtg
      241 cgctattggt ttaccggttt tgaccggagc gacatcatgg ctgacgtttt cgaccacaat
      301 ctatttgaag acaagacatt cgggcagatg cccaagacca ctccgaaaat tcttatcaat
      361 gctacatcgc taacggaaaa cggagcgttg tttcttttca ccgacgaggc tttcaacaag
      421 attggatccg ggctcgataa gtacgcggtc tctagagctg tgatggcatc cggcgctttt
      481 ccgggagcct ttcacaacgt tacgctgcgc gactatacgc aaccaaaaca atacgtccat
      541 ctgtttgatg gcgggccttc agacaattta ggaatcaaca gcctccgcaa agtcgcccga
      601 gagctgtatc tagaacctga aagtgcgcgc cctaagaaat gtctggtctt gatagtcgat
      661 gcttacacag aaacgtggaa tttatcgcgg gaagactccg atacgcgaaa agctatcacc
      721 gattttgtca tcgacaccaa tgctttggag gcaagcagcg tgctgctcgc gttacgaaga
      781 cataaactgt tggaggagat gggaatcaga ccggatggca acacagcgcc tgatgtatac
      841 cagtcattcc ggctcttcgc tgaagcagat gatcctgaat taaagaaaaa tccgaaacta
      901 caagacgtcg agtgtcacgc gtggctgatc acgtttcaac ggctgcagca tttgacacat
      961 ttgccgaacg cggggaccat tagtgcaatc gtcaataata taccgacccg ctacacgttg
     1021 ctggcgcctc acggcttggg cgcggctgaa gcacaggagg cgttgttcac cgccgccgag
     1081 atgctaataa aagaagacaa agagacactt gccgcgatct gtaaggattt tgccgacaac
     1141 tttgccgaaa tgaagtgtcc gaaataacat ttgtttgatt tcggacgtcg tggctgcagg
     1201 ctgaaagaag ggttgattag aacagagact gtgtttgacc cgagaaccgt tctgtccggc
     1261 gcgcaatgag cgtctagttg actttctttc tccgggtacc cgcgataaac atcccgtgat
     1321 gattaccctt acatcacgac taagcgcgac ttgagctttc ctttggcggc gcgttcgaaa
     1381 agcctatcga tcttcttctg agcttgctgt ccgatctgct catatccaca ttcatggcag
     1441 acggtgcacg ggatattttg gatctccatt gaatccttat gcattgtcgt tcggcccaaa
     1501 taagtcctcg agccgcactg tgagcatttc ccaggcatgg aatccaatgt agcccgaatt
     1561 gaattggctg ccattacctc tagcgccaga tctactgctg cgcggccact ttgcgccggc
     1621 cgatggcggc ttcggctctg gggctgatcg cgcgctgcac ggcaacgttc agtagcgcgg
     1681 gttttcccgc cgctaaggcg cgcttcaatc ccggctccag atcctccggc cgctccacaa
     1741 gctcgccgta accgccgagg ccctgcacca cctgatcgta gcgggtctgc agcaagtcgg
     1801 tggcaacggc ccggccgtag aggccaagct gaatttgccg gtcgatgccc caggcggcat
     1861 cgttgcccag tagcgctacg atgttgagtt tgtggcgcac ggcggtgtcg aattccatgg
     1921 cgttaaagcc gaaggcgccg tcgccgctca gcatgaacac gcgcgaatcc gggtaggcga
     1981 tcttcgccgc cagcgccgtc ggtaacgaag agcctaacat gccgagactg gaaacgttga
     2041 accagcgctt tggtttgagc gcggggagaa atgatcgtcc gaagtgacag aagtcgccgc
     2101 catcgaagac caggcaatcg tccgcgcgca gaatcgtctt gagcgtctta tggacgaaca
     2161 aggcatgcat cggcgtttgc gaccgggcca gatcctcggc ccactc
//
LOCUS       DASUTG010000283         7101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_341862, whole genome shotgun sequence.
ACCESSION   DASUTG010000283 DASUTG010000000
VERSION     DASUTG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341862"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..533)
                     /locus_tag="VF089_06395"
     CDS             complement(126..533)
                     /locus_tag="VF089_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX6173626.1"
                     /translation="MRLALLFLGLPLLELYILIKIGSRLGAFLTIGLIVFTALLGLLL
                     ARFEGLRTLHQIRQSLSQGIVPAEEMVDSVLIFVGGVLFVVPGVITDIAALVLLIPFT
                     RTIFKRWLRRRFDRMVAKGNVRLQYHRSTPDDY"
     gene            complement(536..1453)
                     /locus_tag="VF089_06400"
     CDS             complement(536..1453)
                     /locus_tag="VF089_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX6173627.1"
                     /translation="MRICSLLPGATEIACALGLADDIVGVSHECDFPAAVRSKPVMVR
                     SRIDPTMIGSGEIDRQVGDLLRSKQSLYALDEKLFKDSEPDLILTQGLCDVCALDYND
                     VVRAACSLAQEPTIVSLDPHCLADILNDVLRIGEVTHRLRQAESLVQELRLRIQAVRD
                     RTARSASQPRVACLEWFDPLYIAGHWVPEMVQIAGGNDILGTAGEPSVKIEWEALIAS
                     RPEVLILMPCGFDLERAIRESSLLKDRAGWQTLPAGRDGRVFAVSGADYFSRPGPRLI
                     DGLEILGQLIHPELSPAPISSDLAKRVNL"
     gene            1931..2137
                     /locus_tag="VF089_06405"
     CDS             1931..2137
                     /locus_tag="VF089_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX6173628.1"
                     /translation="MKAKRKKKDPEIVMRGGKPTAVILGIEEYRELLERVEDAEDLKA
                     LKAMRKRPLKFRKLEDFLKASAGV"
     gene            2130..2384
                     /locus_tag="VF089_06410"
     CDS             2130..2384
                     /locus_tag="VF089_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX6173629.1"
                     /translation="MYEVLIERSAERDLKSLPTPLFHGIVSRIRALADNPRPSGCHKL
                     AGSKNDWRIRIGDYRAVYEINDARKLVRIFRIRHRSEVYR"
     gene            2818..3081
                     /locus_tag="VF089_06415"
     CDS             2818..3081
                     /locus_tag="VF089_06415"
                     /inference="COORDINATES: protein motif:HMM:NF039814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5647 family protein"
                     /protein_id="HEX6173630.1"
                     /translation="MNVFEQKNAELVTEFDRYVREHPDFAERIPANALVAMLIEGDQE
                     FNRWSQEGAQRQAEKQQPIVYVKIKRIQPIRSRIEEIEVSASG"
     gene            complement(3893..4177)
                     /locus_tag="VF089_06420"
     CDS             complement(3893..4177)
                     /locus_tag="VF089_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173631.1"
                     /translation="MQNITKRLRVWAIIVALLLLVPLVAMQFTDEVVWTRSDFVVAGV
                     LLFGSALVYELATRNMSHAKKRIAVGIVVVVVLLWLWAELAVGVFTNWGS"
     gene            5377..5679
                     /locus_tag="VF089_06425"
     CDS             5377..5679
                     /locus_tag="VF089_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173632.1"
                     /translation="MNTEPEKELKDAGFPHERCNTERVYCEDPAHFPTLSELIEACGD
                     KFHRLETPIKATKREKTWWYAHSTEPEDFTVGDTPEEAVARLWLALNKKDVGEDKA"
     gene            complement(6345..>7101)
                     /locus_tag="VF089_06430"
     CDS             complement(6345..>7101)
                     /locus_tag="VF089_06430"
                     /inference="COORDINATES: protein motif:HMM:NF037126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173633.1"
                     /translation="LWSALKIHYWDTFRTQVSYGDTVGKCFNQQLRNLCERLWLDFFK
                     LPLDNLSPDREVTYARLRDLFFKWSWDEVYDFIEFVAQNYSVDPANKEFMAFCNNILE
                     REMSAYRFVGGAIAPITSEEEISAINDAMTVKISPVRAHLNSALEKLTDRKHPDYRNS
                     IKESISAVEALVQKTTHSDQGTLGQLLKILESKTGLHPSLKGAFDKLYGYTSDADGIR
                     HALLEEDKVTFEQAKFMLVVCSAFTNYVIGTLK"
BASE COUNT         1925 a         1780 c         1757 g         1639 t
ORIGIN      
        1 aaaagacctc agtggcagac gcgcgactta caaggatgaa agcggaaatc tgaaagcgga
       61 attcagtttt cagctttcat aattcatctt ttcgaaagca tatcagtttc ttttcagcgc
      121 ttctcttagt aatcgtccgg agtgctgcgg tgatactgaa gccgcacgtt acctttcgcg
      181 accatgcggt cgaatcgccg ccgcagccag cgcttgaaaa tggttcgggt gaaaggaatc
      241 aacagcacca aagcggcgat atcggtgatg actccgggta cgacaaaaag aactccaccg
      301 acaaagatca agacgctgtc gaccatctct tctgccggca cgataccttg cgaaaggctc
      361 tgcctgatct gatgcaaggt tctgaggcct tcgaaacggg caagcaacaa tcccaacaat
      421 gctgtgaaga caatcagccc gatggttaga aacgcgccga gacggctgcc gattttgatc
      481 aggatgtaga gttccagcag aggcagtccg agaaaaagaa gagctaaacg catgcctaaa
      541 gattaactct tttggcgaga tcagacgata tcggcgccgg agataattcc ggatgaataa
      601 gctggccgag gatttccaaa ccatcgatga gacgcggccc gggccggcta aaataatccg
      661 cgccgctgac ggcgaaaacg cgtccgtctc tacccgcggg caaggtttgc cacccggcgc
      721 gatccttcag cagcgaactc tcacgtatcg cgcgctcgag atcaaagccg cagggcatta
      781 gaatcaagac ctccggccgg gatgcaatca gagcttccca ttcaattttc accgatggct
      841 cgcctgcagt tcccaagatg tcgttgccgc cggcgatctg caccatctcg ggtacccagt
      901 ggcccgctat gtagagcgga tcgaaccatt ccaaacaggc cactcgcggt tgtgacgctg
      961 atcgcgccgt tcgatcacgt accgcttgta ttcgaagtct tagctcttga accaatgact
     1021 cggcctgccg caacctatgg gtcacctcgc cgatacgcaa cacatcgttc agaatatcgg
     1081 cgagacaatg cggatcgagc gagacgatgg ttggctcttg cgcaagcgag cacgccgctc
     1141 ggacgacgtc gttgtaatcc agcgcgcaaa cgtcacataa gccttgggtg agaatgagat
     1201 cgggttcact gtctttgaat agtttctcat caagcgcgta gagactttgt ttcgagcgca
     1261 gcagatcgcc cacttgtcga tcgatttcgc cgctgccgat catggtcgga tcgatccggc
     1321 ttcttaccat cactggtttg cttcgaactg ccgccgggaa atcgcattcg tgactgacgc
     1381 cgacgatatc gtcggccagc ccgagagcgc acgcgatctc agtggcgccc ggcagcaagg
     1441 agcagatccg catggatgaa tgattagcgt atcggtagcg catttgccac acgagccggg
     1501 tatgtcccag ccacttggtc gcgctaagga tcttgacgcg attcgctagg gctgtatcag
     1561 gttcaagcgc taaaaagtcc gggtgtcgcg gttgataccg cgagagattt cggtctaaac
     1621 acagaaatcg ccgatctcct cgcacgcaag atttgtcgcc agctcggtat cccagatcca
     1681 aaatagcggt aagcccgctt atgctgttga aatttcgacg tttcgggaat tctcgaaacg
     1741 tggaagttct cgcccaaatg tcttttctcg cattcttaaa ttagttaagt ttaacagcgc
     1801 gattcgctac cgttcgaaca gagcacgcga ttggccgtgg aatacgagcg ctcttgaccg
     1861 agtggcaggc tgatcatttg aaggaatttt atgacgatgg tactctcgtc agaactaggg
     1921 gtgaacgtcg atgaaagcca aacgaaaaaa aaaagatcct gaaatcgtga tgcgcggagg
     1981 aaagccgaca gcggtcattt tgggcatcga ggagtatcgt gagctactcg aacgagtaga
     2041 agatgcggaa gacctcaagg cgctaaaggc aatgaggaag agaccactca aattccgcaa
     2101 gctcgaagac ttcctgaagg cttctgctgg tgtatgaggt cctgatagaa cgtagcgctg
     2161 agcgcgatct aaaatccctg ccaactcctc ttttccacgg tatcgtttcg cgcattagag
     2221 cgctcgcaga caatccacgc ccatctggct gccacaaatt agccggctct aagaacgact
     2281 ggcgaattcg gatcggcgat tacagagcgg tttatgagat taatgatgcg cggaaactcg
     2341 taagaatttt caggatcaga caccgcagcg aagtctatcg ctaacggaag accctatccg
     2401 tactggtctc atgccgaacc acctcggcca gcctcaaggt gcccagatta ttcttggtgc
     2461 gcggcacgct ccggccgata gcgttcgcga tcctatggtt ctcggggcgc ccttatcgct
     2521 gctcagtaaa gagaccacgg aggtgcacga aagtgccata atcataaaac cgataaaggg
     2581 aatttagtcg catttatggt gcagctggaa gtggaagtgg aagacggaat ttggaagccg
     2641 gtgattcggt acgattgcgc ccacgacttc gcccatcgtg atcggtacaa cttaagaggt
     2701 gatcaggaca agcaagagat cgcgttgagt tacgctgagt ctctagatct cgcggacaaa
     2761 gacatcaacg ataattggga tatttaccag caagcgcttt ctaagaggag attatccatg
     2821 aatgttttcg aacagaaaaa tgccgagctc gtcactgaat ttgaccggta tgttcgggag
     2881 catccggatt tcgccgagcg gattccggca aatgcattgg tggctatgtt gattgaaggg
     2941 gatcaagaat ttaatcgctg gagtcaagaa ggcgcacaac gccaagctga aaaacaacag
     3001 ccgatcgtgt acgtgaagat caagcggatt caacccatcc gatcgcgcat tgaagaaatc
     3061 gaggtcagtg cgtcaggtta gactgatcgc gatgctgtaa tgctcatttc gccgaaacgg
     3121 aggtatggcg ccgtgacggt gccattagcc tttagcttaa gagtactagc gttttccttc
     3181 ctacctatcc tttgagattt ctgaatttca aacctgttca gtccttctcg catctttcgc
     3241 ggttcgccgc tcgtttcgag gttctggaat tctcgaatcg aaacgtggaa gttcctctct
     3301 ttcgttgaac gattgaactg ttgaacgttg gacgatttga atcggggtga acggcttgga
     3361 cggagcgaag cggttgaacg atttgaacgt tggaacactc caccccatcc ctggaccgcg
     3421 agatgtccca tatcatcacg agttcgcgag cagaacagaa atttccctag cccagaacag
     3481 atgcgacggg acgagtctgg aagcattcaa gaggaggaaa tgtcaaaagt aaagacgcga
     3541 taccccatcg cgcgttcctt ggggcttggt tcttccgcct atttgcgaat acacagggta
     3601 caccccaaaa cagcaggacg atgtgtttca gaatctattt gcgttgcggg aagtgcaaaa
     3661 gtgacgcggg cgggaggtag ttactctcca gcactgtaag gacctttcca cggggaattc
     3721 gctgaatttc tagagcgcat cattgcggag gctgcggata tgggaatcgt gatgcctccc
     3781 gccgatcctt cgaaatcgac gcggagactg gacgcatgag cttagcctat tataaatcca
     3841 tctcgtaagc tctgccttat cgaacaattc acctacgctt gggtatatcg aatcaactac
     3901 cccagttagt gaagacgcca acggcaagct ctgcccacag ccagaggagc actacaacca
     3961 ccacaatgcc gacggcgata cgcttcttag catggctcat atttcttgtt gccagctcat
     4021 acacgagagc ggaaccgaac aaaaggacgc cggcgacaac gaaatctgac cgggtccaaa
     4081 ccacttcatc ggtgaactgc atcgcgacca agggcacgag cagtaggagt gctacgatga
     4141 tggcccacac tctcagtctt tttgtaatgt tttgcataat gaaacgctta gacgcaggag
     4201 tttatcccca caatccccag cttatcccct aagaatagaa aaagccggac ttgttcagtc
     4261 tcggcttttt cgtttacgat tgagttatgg catcacatta attgtgattc aaatttccaa
     4321 gaccggcaac ggacggtttt tatttgtcat taaaaaatag aaaagaagac ctaaatcgtg
     4381 aggcaatgag agccgtgggg caaccagggg gcctgacaaa gcttacatgg gtgaagagta
     4441 tgcgtcgctg acggtgaaaa ttcgcatcgc gcggttttaa aacggaagta cgagcttccg
     4501 ataccatctt gaacgacacc tttaccactt caatgtgcga ggattcggcg gatttcgccg
     4561 cgcgtttgtt tcaagaactt gtaacctttg accgttacta atgttactcc tttgattctt
     4621 gttcatactc tttattactt cccttagaag tccaggggtt ccccccgata tggttttcaa
     4681 ctccgaatta caatttattt ccggcgaaat cgcagaattt tgcggcgaac caaaaaagct
     4741 tggaaaatac ctcggcatcg ttaaacgtat tggcaaacaa aaggctttcc aaattctttc
     4801 agagctgaaa aaaagaggaa gctgagtacc ttcataaacc gcccgccgaa cgcccatcac
     4861 cgatcaaaga ccgcgccgcc tactttatct ggaaagcaaa aaacggctga atccggctga
     4921 agataccagg gaaacttact cgataaatcg agccttcagc cagatccaac caagccataa
     4981 aaccatgctt ccccgcccaa ggtccgccga gaagagggag cttccctcca cttagtaata
     5041 gacgaagcag agctgtgaaa cttatcccca acaacctggg caatatgtgt gggaagatat
     5101 gttcatggaa gaacaagaaa tgagactgat aatcgacgat ttgaacagag ttggaagcta
     5161 tgcacaggcc aagcttttac tgagccgtct cccctagggc gttcaagacg ctctccgctc
     5221 aaccagccgc gccaagcacg taattgaaat caagaccagg attattactc tcttacgaga
     5281 ccgcttcgag ctttcgttcc cccccatcgg ggagctcctg ggatactccg accattcagg
     5341 agttattcat aactattaca aagctaaacg caccaaatga ataccgaacc agaaaaagaa
     5401 ttaaaagatg cgggattccc gcatgagaga tgcaatacag agcgagtcta ttgcgaagac
     5461 ccagcacatt tccctactct ctctgaactc atagaggctt gtggagataa atttcatcgt
     5521 ttagaaacgc caatcaaagc aactaagcgt gaaaaaactt ggtggtatgc gcattcaacc
     5581 gagcctgagg attttacggt aggcgacact cccgaagaag cagtcgcccg tttatggtta
     5641 gcgttaaata aaaaggatgt gggggaggat aaagcttagg cgagccgaca cccttttttc
     5701 tctgtacctc cgcaaaaacg cggctaccga tgtgaatttg acaatgcatt ctttcctgaa
     5761 ggccgagggc ttaccgtttc ccatttttgg agccgaggca aggattccgt tcgcttcgat
     5821 gaagagaatt gcgacatcct ttgcgtctca caccatgctt attttgaaga gcacaaaacc
     5881 gaatatgaaa cttggaaaaa agaccgcatg ggagagaaag cattcaacct cttaatgctt
     5941 cgcgctcata cctaccacaa gaaagacgcc gcgccgattc tcatgtggct tacgctggag
     6001 cgcttaaaga acataatcag aggcatcatc ccgactcaat cactctcacc gaggccgctg
     6061 agagcttaga ggaaaaagga ttactcccga atctccttga tccaaaccaa acaaccatga
     6121 aggctaaccc ttgatgatcc accagatccc cgttaatcac aatcaccggg atcactgccc
     6181 agaaagtgta aagcagaaga aaattcgttc catggtgcgt tgaggctgat catgaaagca
     6241 aaagcgccaa gtaaccaaat ggctgaaacg acgattatgg cgcgccgtat tctgaccatc
     6301 ggaattcatt atcactgatc gggggctgaa caaaccgaaa ccatctattt gagagtgccg
     6361 attacataat ttgtaaaagc agagcagacg acaagcataa atttggcttg ctcgaaagtc
     6421 actttgtcct cttcaagaag ggcatggcga atcccgtcag catcggatgt atacccgtag
     6481 agcttatcaa aagcgccctt tagggaaggg tgaagaccag ttttgctttc cagaatcttg
     6541 agtaactgcc ccaatgtgcc ttgatcagaa tgtgtagttt tttggaccag cgcctcgaca
     6601 gccgagatcg attccttgat cgagtttctg tagtcgggat gttttcgatc ggtgagtttt
     6661 tccaatgcgc tgttgagatg tgcccggact ggagagatct taactgtcat ggcatcattg
     6721 atggctgaaa tttcttcctc agaagttatg ggcgcaattg cgccaccgac aaatctgtac
     6781 gccgacattt ctctttccaa gatattatta cagaatgcca tgaattcttt gttcgctggg
     6841 tcaacagaat agttttgagc gacaaactcg atgaaatcat acacttcatc ccaagaccat
     6901 ttgaaaaaaa gatcgcgtaa ccgcgcgtag gtcacctccc ggtcgggaga caaattgtca
     6961 agcgggagtt taaagaagtc gagccataat ctctcacaaa gattccgcag ctgctggtta
     7021 aagcatttac caacagtatc tccgtaggag acttgagtgc gaaatgtgtc ccagtagtgg
     7081 atctttaaag cgctccacaa a
//
LOCUS       DASUTG010000284         6460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1676179, whole genome shotgun sequence.
ACCESSION   DASUTG010000284 DASUTG010000000
VERSION     DASUTG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676179"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1768)
                     /gene="glgP"
                     /locus_tag="VF089_06435"
     CDS             complement(44..1768)
                     /gene="glgP"
                     /locus_tag="VF089_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017838793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6173634.1"
                     /translation="MMTEPTAFSVPATIAYFSMDVAIDSDIPTYSGGLGILSGDTLRS
                     AADLGLPMVAFTLLHRKGYFDQRLDAVGNQLESPSQWSPEKFLEALPSRVSVMIEGRE
                     VKIRAWQYRFHGVMGYTVPLLLLDTDLEENDSRDRILTDHLYGGDDRYRLCQEAVLGL
                     GGVQMLRALNYPGFKIYHMNEGHSALATIALLEQLSASRRFSETEIETVRRQCVFTTH
                     TPVPAGHDRFPADLVDQVLGEDRAAVLRQVGVMDGSLNMTELALRLSGYVNAVSMRHG
                     EVSREMFPGHRVEAITNGIHAESWIAKPIATLFDRHIPDWRRDNCYLRHAIGFPLQQV
                     REAHAEAKRELLQQVRWLTGSQLDDAVFTLGFARRATAYKRGDLLFTDLDRLKNIARQ
                     VGPLQLIYAGKAHPRDDGGKAIIRRIFEAAASLANDVRVVYLENYDMALGKMLCAGVD
                     VWLNTPLRPQEASGTSGMKAALNGVPSLSVLDGWWIEGHIEGVTGWSIGELGGDGGTT
                     TEVDSLYYKLGEVILPLYYNAPEKFAHVMRSAVAFNGSFFNTQRMIFQYLRNAYLPAW
                     TKAQASLL"
     gene            complement(1908..2627)
                     /locus_tag="VF089_06440"
     CDS             complement(1908..2627)
                     /locus_tag="VF089_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173635.1"
                     /translation="MSELVAPGRASTVIVARPAADGGFEVLMTRRSPHLKVLAGFLVF
                     PGGAVEEDDWSDRMLARCRGLSPTEAQQILASAMTPEQAMGHWVAAIRELFEETGIHF
                     FTKQGDSLGLNVDGLRERLAQSREALWRRRLALADLLESEQLLCDVGRLTYLFHRITP
                     NHHPVRFDTRFYVGSLPQGQTPLDYSEEVAESVWLSPQDALRLSEAGGLPMMPPTLIA
                     LRSLAELGSWQNFIVTYRLET"
     gene            complement(2696..3481)
                     /locus_tag="VF089_06445"
     CDS             complement(2696..3481)
                     /locus_tag="VF089_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173636.1"
                     /translation="MAFSQRTPPLILLTLLLLPVWIGTARGQGNTSRRASSIPRGATA
                     DAELARLRADVIQKMRDSRAGAEKLIALREDEVKKLTMEYEKRRQFYDEGLISKTELN
                     QSARSLGEAIARLQDDKRWLSDQDIAISEASMRDDLQHSPSLGAGYSETASLIRFNGD
                     RVWSIADAPKIEIFFFETFGRGLPVSALGQTGIHDRLQIDHRNAMDVALHPDSREGRS
                     LMFHLRQSRIPFIAFRNAVPGSSTGAHIHIGRTSPPLRAAMRQ"
     gene            complement(3554..6235)
                     /gene="polA"
                     /locus_tag="VF089_06450"
     CDS             complement(3554..6235)
                     /gene="polA"
                     /locus_tag="VF089_06450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6173637.1"
                     /translation="MSAKIKTQTTRKPPGSIKHTSSRNNLRKIILIDGHSLAFRAFFA
                     LPDTLITSSGQVTNAVYGFTSMLIKLLADERPQAVIVCFDKGAPQFRLDRYAAYKAGR
                     AETPDLFRQQLPLIREVLQALRIPIVELEGYEADDLLATLTKNCRGEGCEVIVVTGDR
                     DILQLVGDGVSVIMTRRGISDVVRYDTTTVIERYGVSPDQWLDFVALKGEPSDNLPGI
                     PGVGDKTAAQLVKKYGDIEGIIAHADELTPKLRDSLKAHAEQVRINKELGRLLNDVAL
                     EFDSAKLCLDRWDDEEVRKLFTSLEFRTLHERLRALNMHVAAPIGAALEPKSISELRS
                     ADEIKQDGAVALAWNENWLAICDGPGVAHMLSRSAAMDRLAEFLQDPARLKVAHDAKS
                     ICRLALAAGIEIQSLYCDTKIAAYLLEPGSPSGYTVADAARRYLGVSLNGDRPEPEIG
                     EQSTLAFGADEQERIAREAIAIRGMVPLLQEQLRDQGIWELATTLEFPLIGVLARMEH
                     DGILIDRDYLEKFDADLGHKVTALERAIKDLAGEDFKVNSQPQLQRILFEKLALPKTR
                     KIKTGYSTDAAELEKLAGVHPIIPVLLEYREVSKLKNGFTEMLLELIDSKSNRIHTTY
                     EQTIAATGRLSSTAPNLQNIPIRGELGRQIRRAFIAPPGHVLLSADYSQIELRVLAHL
                     SQDETFLQAFAQGHDFHATIAAQVYGVALADVTPQMRNQVKQFSYGIAYGMSTFGVSQ
                     RLGVEMDVAASFVEAYYAQFPKVKEFLTAQVNQAKIDGFTTTMFGRRRYLPELGSSNF
                     RLRALGERMALNTPIQGSAADIIKKAMINVGEALRKEPLAKMLLTVHDELVFEVPVEH
                     LDQARALIDKSMNQAADLRCGLVVDIHVGPNWAEAHA"
BASE COUNT         1515 a         1788 c         1686 g         1471 t
ORIGIN      
        1 catgtgcacg gctgatggca aatagtgaag gccgggacaa atattagagt aacgacgctt
       61 gggcctttgt ccaagccggc aggtaagcat ttctgagata ctgaaatatc attcgttgcg
      121 tgttgaaaaa cgagccattg aaagcaactg ctgagcgcat gacatgagca aatttctcag
      181 gcgcattgta ataaagcggc agaatcactt ctcccagctt gtagtaaaga gaatcaactt
      241 cagtagtggt gccgccatcg ccgcccagtt cgccgataga ccacccggtc actccctcga
      301 tgtggccctc gatccaccag ccatctagaa cgctcaagct gggaactcca tttaacgcag
      361 ctttcatacc gctggtgcct gaggcttcct gaggtcgtag cggggtattc aaccagacgt
      421 cgacgcccgc acaaagcatt ttgccgagcg ccatatcgta gttctccaga taaacgacac
      481 ggacatcatt ggccagagat gccgctgctt caaagattcg acggatgatc gcttttccgc
      541 catcatcgcg gggatgggct ttaccggcgt aaattagctg cagcggtccc acctgtcgcg
      601 caatattctt caaccgatct aaatcggtga agagcagatc accgcgctta tacgccgtgg
      661 cgcgccgcgc aaaaccgagg gtaaagacgg catcgtcgag ttggctaccg gtcagccacc
      721 gcacctgctg caatagttct ctcttggcct cggcgtgagc ctcacgaacc tgctgcagtg
      781 gaaacccaat cgcgtgccga agatagcaat tgtcacgccg ccaatcgggt atgtgccggt
      841 caaataaggt ggcgattggt ttcgctatcc aggattcggc gtgaatgcca ttggtgatgg
      901 cctctacacg atgcccgggg aacatctcgc gcgagacctc gccatgcctc atcgatacgg
      961 cgtttacata gccggacaaa cgcagagcga gctccgtcat attgagcgaa ccgtccatga
     1021 ctcccacctg gcgcagaact gccgcccgat cctcacccaa cacctgatcc acaaggtccg
     1081 cgggaaagcg atcatgaccc gccggaaccg gcgtgtgagt tgtaaaaacg cactggcgcc
     1141 gcaccgtctc gatctcggtc tcagagaaac gacggctggc tgagagctgc tccaacaatg
     1201 cgatagtcgc taatgcggag tgtccttcgt tcatgtggta gatcttgaag cctgggtagt
     1261 ttagtgcacg taacatctgt acgccaccca atcctaagac cgcctcctgg caaaggcggt
     1321 aacgatcatc gccgccatag agatgatcgg tcagaatccg atcccgtgaa tcgttctctt
     1381 ctaggtccgt gtcgagcagt aataaaggga cggtgtagcc catgacacca tgaaagcggt
     1441 actgccacgc gcgtatcttt acctcccggc cttcgatcat caccgagacg cgcgacggca
     1501 atgcttcaag aaacttttct ggagaccact gcgagggact ctctaattga ttaccgacag
     1561 catcaagccg ctgatcaaag taacccttac gatgcaggag cgtgaacgca accatcggaa
     1621 gccccaaatc agctgccgat cgtaatgtgt caccggacaa gatcccaaga ccgccgctgt
     1681 aggtgggaat gtctgagtca atcgccacgt ccatcgaaaa gtacgcgatc gttgccggaa
     1741 cagaaaacgc agttggttca gtcatcatcc tttgattctt gtgtttgcca cgttaaatct
     1801 gaggtcgatc gattggacag gaatccatgg cttggttttt tgtacctgag aatgatatca
     1861 cgggtgacaa aacacggcaa ttcttgcggc taagttattg gtaaaactta tgtctcgagt
     1921 cgataagtaa ctataaagtt ttgccaggat ccgagttccg caagcgaccg caaagcaatc
     1981 agcgtcgggg gcatcatcgg tagacctccc gcctcggata atcttaaagc gtcttgcggg
     2041 ctgagccaaa cgctctcggc cacttcctcg gaataatcca gcggtgtttg tccttgcggt
     2101 agggaaccca catagaaccg ggtgtcaaag cgcaccgggt gatggttcgg agtaatacga
     2161 tggaacaagt aagtcagccg accgacatcg caaaggagtt gctcggattc caacaaatcc
     2221 gccaaggcca atctcctacg ccataacgcc tcacgacttt gcgccagacg ttcccgaagg
     2281 ccatccacgt ttaacccaag tgagtcgcct tgcttggtga aaaagtgaat tccagtctct
     2341 tcaaacagct ctcgtattgc tgcaacccaa tgacccattg cttgttccgg agtcatggct
     2401 gaggctagga tctgctgagc ctcggttgga gacaaacccc ggcagcgggc caacattcga
     2461 tcggaccagt cgtcttcttc cacggctccg cctggaaaca ccaaaaagcc cgccagaacc
     2521 ttcagatgcg gcgaacgacg agtcataaga acttcaaagc cgccgtcggc tgctggtctg
     2581 gcgactataa ccgttgaagc tcttccggga gccactaatt cgctcatgaa atcctgatag
     2641 actggccgat aaccttaaac ggactgcatc tgccgctatg ccggacggca aggactcatt
     2701 gcctcatcgc ggcccgaagc ggcggcgatg ttctgccgat atgaatgtgc gcgccggtcg
     2761 atgagccggg aacagcattt ctaaacgcga taaacggaat tcggctttga cgtaaatgga
     2821 acattaacga ccggccttct cggctgtccg ggtgaagcgc aacatccatc gcatttctat
     2881 gatcgatctg tagccgatca tggatgcccg tttgacccaa agcactgacc ggcaagccgc
     2941 ggccgaaagt ctcaaagaaa aaaatttcaa tcttgggcgc atcagcgatg gaccagactc
     3001 gatcgccgtt aaagcgaatt aaagaagcag tctcgctgta accggcccct aagctgggcg
     3061 aatgctgcag gtcgtcacgc atcgacgctt cactaattgc gatatcctga tcgctaagcc
     3121 accgtttatc gtcttgcaaa cgagctatcg cctcgccgag agagcgcgcg ctttgattta
     3181 gctctgtttt tgagattaac ccttcgtcat aaaactgccg ccgtttttca tactccatcg
     3241 taagcttttt gacttcgtcc tcacgcaaag caattagctt ttcggctccc gctcgtgagt
     3301 ctctcatctt ttgtatgaca tcggcgcgca gtcgcgccaa ttcggcatcg gccgtggccc
     3361 cgcgcggaat gctcgaagct cgtcttgacg tattaccttg cccgcgggca gtgccgatcc
     3421 aaaccggtaa tagtaggaga gtaagtagga ttagcggtgg tgtacgttgt gagaaggcca
     3481 tgcagttcct agatccgcgc tagaattccg cccttttact aaacgacgat gtctcaaccc
     3541 cagttcattg attttaagca tgagcttcag cccaattcgg gcctacatga atatcgacga
     3601 caaggccgca acgaagatcc gcagcttgat tcatagattt atcgatcaga gcccgagcct
     3661 ggtcaaggtg ctcgacagga acctcgaaga ccagttcgtc gtgcaccgtc aatagcatct
     3721 ttgcaagagg ttctttgcgg agcgcttcac ccacattgat cattgccttc tttatgatat
     3781 ctgcggcgct tccctgaatc ggggtattga gagccatacg ctcacccagc gcccttagcc
     3841 ggaagtttga cgagcctagt tccggcagat agcgtcggcg gccaaacatg gtggtggtaa
     3901 aaccatcgat cttggcctga ttcacctgcg ccgttagaaa ttccttaact ttaggaaatt
     3961 gcgcatagta agcttctaca aaactcgctg ccacgtccat ctctacgcct agcctttgcg
     4021 atactccgaa agtggacatg ccataggcga tgccataaga aaactgtttt acctgattgc
     4081 gcatttgcgg ggtaacatcg gccaaagcga caccgtacac ttgcgccgcg atggttgcgt
     4141 gaaagtcatg tccctgcgca aaggcttgca gaaacgtttc gtcttgcgac agatgcgcga
     4201 gcacacgcag ctcgatctga gagtaatcag cagataacag cacatgccca ggcggcgcga
     4261 taaatgcgcg gcggatttga cgtcccaact caccacgtat tggaatgttt tgcaggttgg
     4321 gcgcggttga cgacaaccgt ccggtggcag ctatcgtctg ctcgtaggtg gtatggatcc
     4381 gattgctttt gctatcaatc agctccagaa gcatctcggt aaacccgttc tttagtttcg
     4441 agacctcgcg atattcgagc aggaccggga tgatcggatg taccccggcc agcttctcca
     4501 attccgcagc atcggtggag tagcccgtct tgatttttct ggtcttggga agcgcgagct
     4561 tttcgaatag aatccgctgt agctgcggct gcgagttcac cttgaaatct tcgccggcga
     4621 gatctttgat tgcccgttcg agcgcggtta ctttgtgtcc tagatcggca tcgaactttt
     4681 caagataatc gcggtcgatg agaatgccat catgctccat cctggccagt acgccaatca
     4741 gcgggaattc caaggtggtg gcaagttccc agatgccttg atccctgagc tgctcctgta
     4801 ataggggaac catgcctcgg atggcgatag cctcacgagc gatccgctct tgctcatcag
     4861 ccccgaacgc aagtgtactt tgctcaccga tctccggttc gggcctgtcc ccattgagcg
     4921 aaacacctag ataacgtctc gccgcgtcag ccaccgtata tccagacggg gaccctggtt
     4981 ccagcagata ggcggcaatc ttggtgtcac agtacaatga ttgaatttcg ataccggctg
     5041 ccaacgctaa acgacagatc gatttggcgt catgagccac cttcagccgc gcaggatctt
     5101 gaagaaattc cgccagtcgg tccattgctg cgctgcggct cagcatgtgc gcgacgccgg
     5161 ggccatcaca aatagccagc cagttctcgt tccaggccaa tgcgacagcg ccgtcctgtt
     5221 taatctcgtc cgctgatctc aattccgaga ttgattttgg ttcgagagcc gcgccgatcg
     5281 gcgccgcaac atgcatgttg agcgctctca gacgttcgtg caacgtgcgg aactcaagtg
     5341 aggtaaagag ctttcgaact tcttcatcat cccagcgatc gaggcacagt ttggccgagt
     5401 cgaattcgag cgctacatca ttgagtaatc gtcctagctc tttattgatc cgaacttgct
     5461 cggcatgcgc tttaagcgaa tcgcgaagtt tgggtgtaag ctcatccgcg tgcgcgatga
     5521 tgccttcaat atcgccatac ttcttgacga gctgcgccgc ggttttgtcg ccaaccccgg
     5581 gaataccagg aaggttatcg gagggctcgc ccttgagggc cacgaagtcg agccactggt
     5641 cgggggagac gccgtagcgc tcgataactg tagttgtgtc ataacggacg acatcggaga
     5701 ttccgcggcg agtcatgatc acggataccc catcgcccac aagttggaga atgtctcggt
     5761 cacccgtgac gacgattact tcgcagcctt caccacggca gttcttggtc agagtcgcta
     5821 gcagatcatc ggcttcatag ccttccagct cgacaatcgg aattcgcaaa gcttgaagca
     5881 cctcgcgaat cagcggcagc tgctgtcgga aaagatccgg agtctcggcg cgacccgctt
     5941 tataggcggc ataacgatcg aggcgaaact gcggcgcgcc tttatcgaaa caaacaatga
     6001 cagcttgcgg ccgctcgtcg gccaggagct ttatcagcat tgaggtgaag ccgtaaacgg
     6061 cattggtcac ctggccggaa gatgtgatca gtgtgtcagg taatgcaaaa aacgcgcgga
     6121 aagcgagaga atgaccatcg atcaaaataa ttttgcgcag gttatttctt gacgacgtat
     6181 gtttgattga cccgggcggt tttcgagtgg tttgagtttt gattttagcg ctcactgtta
     6241 gcagtctacc cgttgaattt acccggagca aacatgccgg atggcacacc ggagaccctc
     6301 ttatggtgat ttgcgactgg atcgtcatga tcaccacgaa gaacacaggc gaacccaccc
     6361 cggtccattg gctcggtcgt catgcttact ccgacagcaa agtgttcggg tcgcgaatta
     6421 atttcccgga ggaggtgcgc ggtagcgatt cgcgaatggc
//
LOCUS       DASUTG010000285         9889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1972429, whole genome shotgun sequence.
ACCESSION   DASUTG010000285 DASUTG010000000
VERSION     DASUTG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972429"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..795
                     /locus_tag="VF089_06455"
     CDS             67..795
                     /locus_tag="VF089_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX6173638.1"
                     /translation="MSKTAGIIIIGNEVLSGKTQDTNSHFLCVELRRLGVEVQKISTI
                     QDEIDLIGEEVAYFAKRFDFVFTTGGVGPTHDDVTIEGIARGFSVPVVRHPEIENRMR
                     QRLGGDVNEARLRMANVPQGAVLLATDALFAPVVNIHNVYIFPGIPRILQERFHAIKE
                     RFRGAPYFLKNVFVRYGEGVIASAMNDLLTKFPDLLLGSYPVLDLPEYKVKVTLESKD
                     SNYLDNALKTLVASLPQDAVHRID"
     gene            985..1635
                     /locus_tag="VF089_06460"
     CDS             985..1635
                     /locus_tag="VF089_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6173639.1"
                     /translation="MAEILNWIMESIRSHGAWSVFIGVIIESVIVPIPSPLIIMGAGF
                     VLISPQFSFFEALVPILLQIVLPGSIASTLGAYIGYGIGYFGGKTLVDRWKGFLGFNW
                     SDVEALERRFQGGRVNTTIFFLRALPIFPLSVISAAAGLLRLPLKQFSLWTFYGAIPR
                     CLLLGYLGWGLGETYQGFARGLDRAEGIVSAILVLTIIGGIVWLRARVRGKMLEKN"
     gene            complement(1685..2125)
                     /locus_tag="VF089_06465"
     CDS             complement(1685..2125)
                     /locus_tag="VF089_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX6173640.1"
                     /translation="MFRIINQPIDLQELVRFVTDPEAGAIATFIGTTRNHNDGRKVIA
                     LDYDGYPEMAEKELARIGAAAHKQWQICRMAIVHRLGPVQITEPSVIIAVSSGHREAA
                     FAACRFAIEEIKRTVPIWKKEVFEGGEVWIGTQTGEPLAQKDEG"
     gene            complement(2132..2377)
                     /gene="moaD"
                     /locus_tag="VF089_06470"
     CDS             complement(2132..2377)
                     /gene="moaD"
                     /locus_tag="VF089_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX6173641.1"
                     /translation="MKIKVKFFAILRERAGTAEVTKDIRDGSTVMDLWQALQQDFPKL
                     NVAGIRLLYAVNQNYVSPDFKLSDQDEVVFIPPVSGG"
     gene            complement(2374..2880)
                     /locus_tag="VF089_06475"
     CDS             complement(2374..2880)
                     /locus_tag="VF089_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX6173642.1"
                     /translation="MSVAEHKARGKKSIRCFVITVSDTRSEDTDISGQTIKSMLEAEA
                     HQIAGYRIVKDEPAQIEALLNEALAAVEVDAVIVNGGTGIAPRDGTYEVVSRSLEKKL
                     DGFGEIFRYLSYLDIGSAAIMSRAAAGAARGKVLISLPGSKGAVTLAMEKLVVPEIRH
                     MVSQLQGT"
     gene            complement(2950..3936)
                     /locus_tag="VF089_06480"
     CDS             complement(2950..3936)
                     /locus_tag="VF089_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173643.1"
                     /translation="MSKFSCALLFSIGIFLFGFFRSEAQQPARTPRIGILIPEAGLHE
                     SQTVKGLRDGLKEAGYKEGETIIVEVEDVKGARSQLQGALNELVSKRTDVIFTTGTRA
                     TQAAMATTKEIPIVFRHPADPVTLGLVKNISRPGGNVTGVAAFSLEANQKRLEVLKQI
                     IPNLRRIHIFYDANNRFSPDNFDAAEKSARQLKLDVLEYPVKTAREFKSSLTQMEVNA
                     GDAIFQIADDLVESQGSVLLEEAKKRKMATMFHSEIWASKGSLAAYGPNYYEMGRQAA
                     DLVQKILKGAHPKDLGVAHANKFDLAINLRTASSIGLKVPPELLKKADKVIR"
     gene            5266..6423
                     /gene="hemW"
                     /locus_tag="VF089_06485"
     CDS             5266..6423
                     /gene="hemW"
                     /locus_tag="VF089_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX6173644.1"
                     /translation="MKPFSLYIHIPYCISKCPYCDFNSHVVARIPERDYADALICELD
                     QYASAPNWHGRKVQTIFFGGGTPSTFTPSSIGAILERAFSSFPVDPNCEITLEANPGT
                     VDYGFFSGYRDAGVNRISVGIQSFQPRLLKFLGRVHTAEEGRKALEVIVRAGFDNFSL
                     DLIFANPGQTLKELEDDLNAALEYRSPHLSAYNLTFEEGTPFHHEYRCGRMSALSEDE
                     EIAMAELIEEKLGAAGLNRYEISNYALSNRHSRHNVNYWQLGDYLGLGAGAHSYLQIG
                     TDPTVRKRWSNEKNPGCYISRVNESRTAISDRENIDQDKAAGEFLFLGLRMISGVSCE
                     VFRSTFGRSPVEFFPKIDTWREAGLMTEKNGNLRLTARGLLLANSIFVEFI"
     gene            6567..7751
                     /locus_tag="VF089_06490"
     CDS             6567..7751
                     /locus_tag="VF089_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6173645.1"
                     /translation="MSLTSPTNLNQLRVDHIGSLVRPAMLKDVFARFDRGAASRDELI
                     QAQDQAIRDVIARQEAHGLPVVTDGEFRRHSFQESFSECVSGFEVPENVSLYYERREL
                     NLTPLERAEQNFDEAGPAIITRRAAVERLKLVRNLPLEEFRYAQSFTQLPVKVTVLGP
                     DRIAQRFKWEGSKNVYNDLDDFVEHVVEIERQMIGELVNAGCKYIQIDAPGYTAYVDK
                     VSLERMRSRGEDPERNLQRSIEADNALIEGFPGVTFGIHVCRGNARTTDPVTGKLVPQ
                     WHREGSYDAIAERLFNSLKHHRLLLEYDSERAGSFEPLRLVPKEKVVVLGLVTTKSAE
                     LESVDELKRRIEQATKYLPVDQLALSPQCGFGGIDSKVMGEEEMWRKLDRIVETAAEV
                     WH"
     gene            complement(7851..8012)
                     /locus_tag="VF089_06495"
     CDS             complement(7851..8012)
                     /locus_tag="VF089_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173646.1"
                     /translation="MWLNEAKAAPDVLEALTEAFAALGKDPRWKVSTLRLDIIRGATA
                     KAEQDRDEE"
     gene            8098..8352
                     /locus_tag="VF089_06500"
     CDS             8098..8352
                     /locus_tag="VF089_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173647.1"
                     /translation="MREGEYVDSLISEYETILGHGELKDAEGGYDESGILKQLSVSAD
                     WSTSGARELLKLADKYGAFMLRNALAIAVVLGKEDGSVGF"
     gene            complement(8567..>9889)
                     /locus_tag="VF089_06505"
     CDS             complement(8567..>9889)
                     /locus_tag="VF089_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEX6173648.1"
                     /translation="SSRIEELVDEAQAQRLKVICFVTGVPGAGKTLVGLNIATRRRDM
                     DQPTHAVFLSGNGPLVAVLREALTRDEVARQKSYGIPVRKGKVAESVKAFIQNVHNFR
                     DDALFDSVPPAERVVIFDEAQRAWNLRQTASFMRRKKNRPGFSDSEPEFLISCMDRHT
                     DWAVIVCLVGGGQEIHTGEAGIDAWLEAVNSRFPHWHMHISSKLSDTEYAAGKALDVI
                     RHRQNTHFDDSLHLSVSMRSFRADNVSAFVKALLDCEKQPAREAFVRLAHRYPIAVTR
                     DLSRAKQWIRAHARGTERFGLIASSKAQRLKPHAVDIRVEVNPVHWFLNGKEDTRSSY
                     YLEDAATEFQVQGLELDWACVSWDGDLRFTGSGWSFHDFRGDRWCNIANVDNRRYLRN
                     TYRVLLTRARQGMVIFVPPGDPSDPTRSSEFYDSTFNYLTELGVPELP"
BASE COUNT         2237 a         2592 c         2643 g         2417 t
ORIGIN      
        1 catgagctgc agcgaatttg atcgttgagc gatcttgctc aatcacgcgc ttaggaaaga
       61 ttagtcatgt cgaaaaccgc cggcatcatc atcattggca atgaggttct ctcgggcaag
      121 acccaggaca caaattccca ttttttgtgc gtcgagcttc gacggctggg cgtggaggtt
      181 cagaaaatct ccacgattca ggacgaaatc gatcttatcg gcgaggaagt ggcgtacttt
      241 gcaaagcgat ttgatttcgt tttcaccacc ggcggcgtgg gccccaccca tgacgatgtc
      301 accatcgaag gcatagcccg aggattctct gtcccagtcg tacggcatcc agaaatagag
      361 aaccggatgc gccagcgcct cggcggggat gttaatgagg cgcggctgag aatggccaat
      421 gttccgcaag gtgccgtgct tttggccacg gatgcgctct ttgctccagt cgtgaatatc
      481 cacaatgttt atattttccc gggcataccg cgcattcttc aggagcgctt tcacgccatc
      541 aaagaacgct tccgcggcgc gccgtatttt ctcaaaaacg tttttgtcag atacggcgag
      601 ggcgtcatcg cctccgcaat gaacgattta ctgacgaagt tccccgatct tcttctcggg
      661 tcctacccgg ttctcgatct gccggaatac aaggtaaaag tcacgttgga atcgaaagac
      721 tcgaactacc tagataatgc gcttaaaacc ttggtcgctt ctcttccgca agacgcggtt
      781 caccgaatcg attaactctc aggaattcat cgccgtgccg acggcgcccg cttccgctcc
      841 cgtgctcaag gtgttgagac attctgatcg ccggtaatgg ccccgagctc tgaggccagc
      901 ctggcggaac atggcgcaaa cgtttccctt aacttaaatt ggtgaagctg ttaccctggc
      961 gcaaattcga ttggagaatt aaacgtggcc gaaatcctaa attggattat ggagtcgatt
     1021 cgctctcacg gagcgtggag cgtcttcatt ggcgtcatca tcgaatcggt gattgtgccg
     1081 atcccatcac ccttgatcat catgggcgcc ggtttcgtat tgatcagtcc gcagttcagc
     1141 ttctttgaag cgctagtacc gatactttta cagatcgttt tgcctggatc gatcgcttca
     1201 acgcttggcg cctatatcgg ttatggcatc ggctactttg gcggtaaaac tctggtcgat
     1261 cgctggaaag gatttcttgg atttaactgg agcgatgtcg aggcgctgga acggcgcttt
     1321 cagggtggta gggtcaacac cacgattttc tttttgcgag cactgccgat ttttccgcta
     1381 tcggtcattt ccgcagcggc cggattgctc cgtttgccgc tgaaacaatt cagtctctgg
     1441 acgttctacg gggctatccc gcgctgtctt ttactgggct atcttggctg gggattaggc
     1501 gagacgtacc agggttttgc gagaggcctc gatagagccg agggcattgt ttcagcaatc
     1561 ttggtactga ctattatcgg cgggattgtt tggctgcgcg ccagagttcg cgggaaaatg
     1621 ctcgagaaaa actgatcaac cggcgtgcgc gcaaataacc cgcagttttg ctccagctct
     1681 tccatcatcc ttcatctttt tgcgccagcg gttctccggt ttgcgttccg atccagactt
     1741 ctccaccttc gaaaacttct tttttccaga tcggcacggt tcgcttaatc tcttcgatgg
     1801 caaatcggca cgcggcaaat gcggcctccc gatgacccga ggaaacagcg atgatcacgc
     1861 tcggttcggt gatctgcacc gggccgaggc gatgaacgat agccatccgg caaatctgcc
     1921 actgtttatg cgcggctgcg ccgatacgcg ccaattcttt ctccgccatt tccggataac
     1981 catcgtaatc gagcgcgata accttgcgtc catcgttatg attgcgtgtc gtgccaataa
     2041 acgtagcgat ggcgccggcc tcggggtcgg tgacgaagcg cacgagttct tgtagatcaa
     2101 taggttggtt aataattcga aacattggtt ctcatcctcc gctgactggc ggaataaaca
     2161 ccacctcatc ttgatcgctc aacttgaaat cagggctcac gtagttctga ttaaccgcat
     2221 acagcaggcg aattcccgcg acattcaatt tggggaagtc ttgctgtagt gcctgccaaa
     2281 gatccattac cgtgcttcca tctcgaatat ctttggtgac ctccgcggtt ccggcgcgct
     2341 cgcgcaagat cgcaaagaat ttaactttga ttttcatgtt ccttgcagtt gagaaaccat
     2401 gtgacggatc tctggaacga ccagcttttc catagctaac gtgacggcgc ctttggagcc
     2461 gggcagggaa atcagcactt tgccgcgagc ggctccggct gccgcccgac tcataatggc
     2521 ggcagagccg atatccaaat agctcaggta gcggaaaatc tcgccaaagc catcgagctt
     2581 cttttcgagt gaacgcgaaa ccacctcgta cgtgccgtct cgcggcgcga taccagtccc
     2641 gccattgacg atcacggcat cgacttcaac cgcggcaagc gcctcgttga gaagcgcttc
     2701 aatttgagcc ggttcgtcct taacaatccg atagccggcg atctggtgcg cttccgcctc
     2761 cagcatcgat ttgatggtct ggccgctaat atcggtgtct tccgaacgcg tatcgctgac
     2821 cgtgatcaca aaacagcgga tgctcttctt acctcttgcc ttatgttcag caactgacat
     2881 gttcacctct ggaccgttta gagttgcttt caagaccgta taacaccctc tgcgcggtat
     2941 tcaattacgc taacgaataa ccttatcggc cttcttcaac agctccggcg gtactttcaa
     3001 accgatactg ctggcggttc tgaggttaat ggccaggtca aatttatttg cgtgcgccac
     3061 gcctaggtcc ttagggtgcg cgcctttcaa gattttctga actagatcgg ctgcctgccg
     3121 acccatttca taatagttgg gtccgtaagc agccaggctc cccttgctgg cccatatttc
     3181 agaatggaac atggtagcca tttttcgctt tttcgcctcc tcgagaagca ccgatccctg
     3241 actctctacc agatcatcgg cgatttgaaa tatcgcatca cctgcattta cttccatttg
     3301 agttagcgag cttttgaatt ctctagccgt tttcaccgga tattcaagca catcaagttt
     3361 tagctggcgc gccgattttt ctgccgcatc aaagttatcc ggggagaacc tgttgttggc
     3421 atcgtaaaat atatgtatgc ggcggaggtt tggaatgatc tgcttcaaaa cctccaagcg
     3481 tttttgatta gcttccagtg aaaaggcggc gacgccagtg acgtttccgc cggggcggct
     3541 tatattctta accaggccca gggtcacggg atcagccggg tggcggaaga cgataggtat
     3601 ttctttcgtc gtggccatcg ccgcttgtgt cgctcgggtg cccgtcgtaa agattacgtc
     3661 tgttctcttg cttacgagct cattgagtgc gccttgcaat tgcgaccggg cgcctttgac
     3721 atcctcgact tccacaatga tagtctcgcc ttctttgtag ccggcttcct taagaccgtc
     3781 tctcaagcct tttaccgttt gtgattcgtg gaggccggct tcgggtataa gaatcccgat
     3841 tcgcggggtt cgggcgggct gctgtgcttc tgacctaaaa aaaccaaaaa gaaaaatgcc
     3901 tattgaaaac aagagtgcac agctaaactt agacatgctt tttaccttcc tgagcccagc
     3961 atttgaactc cgctccggac tataccataa gaggcggtct ccaatcgaaa gtggggatga
     4021 tttctcagat acagtcctgt ggattgctgc gggttaactc gatgccgggg cgagcgatcg
     4081 gtggttgcaa cgttcgggag caattgggct tgtcgcctag catgcggcgg tgcggtcgcc
     4141 gcatggccga taacgatcag aaagcttccg tcaaaaagtt tctgaccctt atacgctgcg
     4201 agaaggttct gacgtttttt tgttggtgat gaagggtgta gcgatcagta gtgaaccaag
     4261 ccgtcggctc ctgaccggtt taacttgtgc gaggagaggt acttctcgag tgccgacctg
     4321 gtttgagggc ccagcttccc atctatgggg cctggatcaa agccggcagc tttcagccgc
     4381 tcttgcgctc gccggatttg ctgactatga gagacgttat tgaccgaggt tgttggctga
     4441 gcagaagcgg aagcgcgtgg cgattgtatc tctgtggttt gctcgaaaat cgctttgtct
     4501 aaaattcctg aaatatcgct aagcgcagtg cagcccaatt tgcagcggga aagcgccgag
     4561 ctggttctag cgcctagcat tccatcaatc gggccgggat aaaaaccagc ggtcttaagc
     4621 cgtctttgaa gtagctggat atctgctttg ctgagagtgt tcgcggagcc gataaaggcg
     4681 gcggattgtg tctcggtcgc tggcgcgaag agctccccat gcgcgatttc cagctcattg
     4741 atgattctac acccctcttg aacgcgccaa atcagcgacc ttgttttaga tcccaggatg
     4801 ccatcgatcg gccctggatc gaaaccggcg gttttcagcc gtgcttggag ttgactaatc
     4861 tcctctttac tgagagcttt cgttgacgaa aagggcggtc ggctattttc attcttcacc
     4921 agtgtgggag aaacggaaac caccggctca gtcggtggtt cataaacaac agccggcggg
     4981 tgccgctcag tttgaacttc agactcggaa aggccgctag aaatcgcgca gccagaacta
     5041 aaaatgaaca gaacaagcgt tgcaagattg tagcgctctt gccatctcga atattgcaat
     5101 ttagtgttgt ttacttccat gccgcacgaa tccccgagaa agttagcaaa ccggatgccc
     5161 ccgcggcagc gcggaaatca gtgacgttat tgttatttca gaggagtcga gaagaataag
     5221 agttttgctc gtgggacgaa aacggaaggc tgcaatagcg tcgagatgaa acctttttcg
     5281 ctgtacattc atatcccgta ctgcatcagt aaatgcccct actgcgactt caattcccac
     5341 gtggttgcgc gaatccctga acgtgattat gcggatgctt tgatctgcga gctggatcag
     5401 tacgccagcg cacccaattg gcacggccga aaggttcaga cgattttttt tggcggcggc
     5461 actccgtcga ctttcacgcc ttcaagcatc ggcgccatct tggagcgggc gttttcctcg
     5521 ttcccggtcg atccaaattg cgaaattact ctcgaggcca acccgggcac tgttgattat
     5581 ggtttttttt ccggctaccg cgacgccgga gtcaatcgca ttagcgttgg cattcaatcc
     5641 tttcaacctc ggctgctcaa attcctcggc cgggttcata cagctgaaga agggcgcaaa
     5701 gcgctcgagg tgatcgttag ggcgggattc gataacttca gtctcgatct tattttcgct
     5761 aatcccggcc agaccttgaa ggagttggaa gacgatctaa atgccgcgct ggaataccga
     5821 tcgccgcact tatcggcgta caacctgact tttgaagaag gcacgccttt tcaccacgag
     5881 taccgctgcg gcagaatgag cgcattaagc gaagacgaag agatcgccat ggcggaactc
     5941 atcgaagaga aacttggcgc agccggctta aaccgttatg aaatatccaa ctatgctttg
     6001 tcgaaccgtc actcgcgcca taacgtaaac tattggcagc tgggcgatta tctcggcctc
     6061 ggcgccggcg cgcatagcta cttgcaaata gggacggacc cgacagtgag aaagcgctgg
     6121 agcaacgaga agaatcccgg atgctatatc tcgcgcgtca acgaaagccg cactgccatc
     6181 agcgatcgtg aaaacatcga tcaagacaaa gccgcgggtg aatttttgtt tttgggctta
     6241 agaatgatat cgggagtttc gtgcgaagtc tttcgttcca cttttggcag gtctccggtc
     6301 gagttctttc ctaagataga cacgtggcga gaagccgggc tcatgacgga gaaaaatggc
     6361 aatctcaggc ttaccgcgag aggcctgttg ctcgccaatt ctatttttgt cgaatttatt
     6421 taatgcaatc tgatttgctg taatcattca acggctgtaa gaattcaaat gccgagaatc
     6481 agtctcggat ctctcatccc gtagactcgc ctgtcgtgaa gccggagtta gcctattgag
     6541 caggacgtta atcgaggagg caatctatgt cattgacatc acccaccaat ctaaatcaac
     6601 tgcgtgttga ccacatcggc agcctggtgc ggccggctat gttgaaagac gtgttcgcgc
     6661 gcttcgaccg cggcgcagcc tcccgagacg aattaatcca ggcccaggac caagccattc
     6721 gggatgtcat cgcgagacag gaggctcatg gcctgccggt cgtaaccgac ggtgaattcc
     6781 gtcgtcatag ctttcaagaa agtttctccg aatgcgtctc aggtttcgag gtgccggaaa
     6841 acgtatctct ttattatgag cggcgggagt taaatttaac cccgctcgaa agggcagaac
     6901 agaacttcga tgaagccggt ccggcgatta ttacccggcg cgccgcagtt gaacgcttaa
     6961 agcttgtgcg caatttacct ttggaagaat ttcgttacgc ccaaagcttc acacaacttc
     7021 ctgtgaaggt gacggtgctt gggcccgatc ggatcgccca acgcttcaag tgggaaggat
     7081 caaaaaatgt ttataacgat ctcgatgatt tcgtcgagca cgtcgtggag atcgagcggc
     7141 aaatgatcgg cgaattggta aatgccggtt gcaagtacat ccaaatcgat gctccgggat
     7201 acaccgctta cgtcgacaag gtttcccttg agcgtatgcg ctcgcgtggg gaagatcctg
     7261 aacggaatct gcagcgttca attgaggccg ataatgccct gatcgaaggc ttcccgggcg
     7321 tgacttttgg catccatgta tgccgcggca atgctcgcac caccgatcca gttaccggca
     7381 agctggtacc acagtggcat cgagagggat cgtacgacgc tattgccgaa cggctgttca
     7441 atagcctcaa acatcatcgg ctgctgctcg agtacgacag tgaacgtgcc ggcagtttcg
     7501 aacctttgag gctggttccg aaagagaagg tcgtcgttct aggtctggtc acaaccaaga
     7561 gcgccgagct cgagtctgtc gatgaactca aacgacgcat cgaacaggcg actaagtatc
     7621 tgcccgtcga tcagttggcc ctgagcccac agtgcggctt cggcggcatt gactctaaag
     7681 tgatgggtga agaggagatg tggcgcaagc ttgaccggat cgtcgaaacg gcggccgaag
     7741 tgtggcacta gagcgcgtct atctctcggc ggcagagatg aggaaggtca gctaaaagat
     7801 aaacgtcaga agtccagtcg tagaagcctg cgctgagtgt gggagctttt ctattcttcg
     7861 tcgcgatctt gttcggcttt ggcggtggcg cccctaatga tgtctaaacg caaggtggac
     7921 accttccaac gcgggtcttt gccgagcgcc gcaaacgcct ccgttaacgc ctccaggaca
     7981 tcaggcgcag ccttagcctc attcaaccac aatggtgcaa tcctgaattt ggatcgactt
     8041 ctggagtcgc acagtactat atataggcga ttaaaagacg gagggagagt acggctcatg
     8101 agagaaggtg aatatgtaga ctcgttaatt agcgaatatg aaactattct cggtcacgga
     8161 gaactcaagg atgctgaggg tggatatgat gagtcgggaa tactcaaaca actctccgtt
     8221 tcggcagatt ggagcacatc cggggcacgt gagcttttga agcttgcgga taaatacggc
     8281 gcgttcatgc tgcgcaacgc actcgcgatt gccgtagtac ttggcaagga agatggctct
     8341 gtggggtttt gattcggcgg cgcggcactt acaacgacaa gtgaagcact aatcaggctt
     8401 cgctattgtc gtgcaccaat ttgggcggaa cgcgcacggt agcatccacg atccggaagt
     8461 ggcccgccgt cccgccgtta agagatctcg gctctgattt ccattagtag caggtggaac
     8521 tctccatgaa agtccgcctc aggcatgagc accgctggag gaagactcac ggcaattcgg
     8581 gaacaccaag ctcggtgaga tagttgaaag ttgagtcgta aaactcagac gagcgagtcg
     8641 gatcacttgg gtcaccaggc gggacaaaaa tcaccattcc ttgccgcgcg cgggtgagta
     8701 gtacccggta agtgttgcgg aggtagcggc ggttgtcgac attcgcgatg ttgcaccacc
     8761 ggtcgcctcg gaagtcatgg aagctccatc ctgaaccggt gaaacgcagg tcgccgtccc
     8821 agctcacaca cgcccaatcc agttctaagc cctgcacctg aaactccgtg gcagcgtctt
     8881 cgagatagta gctcgatcgc gtatcctctt tgccgttgag aaaccaatgc accggattaa
     8941 cctcgacgcg aatatcaacc gcatggggtt tgaggcgttg ggctttcgat gaggcgatta
     9001 atccaaaccg ttccgttcct cttgcatggg cgcgaatcca ctgcttggcc cgacttaaat
     9061 cgcgggttac agcgatagga taacggtggg caagtcgtac gaaggcctca cgggccggct
     9121 gcttttcgca gtcgagcagc gccttgacga aggccgaaac attgtcagcc cgaaaggatc
     9181 tcatcgacac agagagatgt agggagtcgt cgaagtgagt gttctgccga tgacggatga
     9241 cgtctaaagc cttacccgcg gcatattcgg tgtcgctgag ctttgatgag atatgcatgt
     9301 gccaatgcgg gaatctcgaa ttcacggctt caagccaggc atcaattccg gcttcgcccg
     9361 tatggatctc ctgtccgcca ccaacaaggc aaacaatgac agcccagtcc gtatgccgat
     9421 ccatgcagga aatcaggaac tcaggctccg agtcggagaa accgggccga ttcttttttc
     9481 gccgcatgaa gctcgccgtt tgtcgcaagt tccacgcgcg ttgtgcctca tcgaagatta
     9541 caacacgttc cgccggagga acggagtcga acaatgcatc gtcgcgaaaa ttatggacgt
     9601 tctggatgaa agctttgacg ctctcggcaa ctttcccctt tcgcactgga atgccatagc
     9661 tcttttgacg tgcaacctcg tcacgggtga gggcttcacg taacacagcg acaagtggac
     9721 cgtttcctga aagaaagaca gcgtgtgtag gttggtccat gtctcggcgt cgagtggcaa
     9781 tgtttagtcc gaccagtgtt ttgcccgctc cgggtacacc cgtaacgaag caaatcactt
     9841 tgagcctttg cgcttgcgcc tcatccacaa gttcttcaat gcgagatga
//
LOCUS       DASUTG010000286         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1825646, whole genome shotgun sequence.
ACCESSION   DASUTG010000286 DASUTG010000000
VERSION     DASUTG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825646"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF089_06510"
     CDS             <1..251
                     /locus_tag="VF089_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX6173649.1"
                     /translation="FYVAGPTSSIDLNCPTGKEIPIEQRDPKEVSHMFGRPLAPEGMN
                     ILNPAFDVTSQELISAIITEKGIIYPPYRQNIENHVRH"
     gene            238..1581
                     /locus_tag="VF089_06515"
     CDS             238..1581
                     /locus_tag="VF089_06515"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX6173650.1"
                     /translation="MSDIEKKDQLEEYFHAAGKPRERWRVGTEYEKVGIDRSSGKAIP
                     YSGPRGVESILKRLIDRFGWEPEEQDGHIIALSRDNAQITLEPGGQIELSGEPCDSIH
                     CTYAEFTQHIRELLEVSEPLDVVFLGLGMQPVSRLDEIEWVPKKRYRIMGPYMPKVGR
                     LGQRMMKQTATVQANIDYRDEKDAMAKFRTGMGLTPIFISMFANSPISDGQLNGYRSY
                     REHIWTDTDNSRSGMLKFAFSADVSFAHYVEYALDVPMYFIIRNKNYVDMTGTTFRQF
                     LAYGWNGQRATLDDWHDHLTTLFPETRIKRYIEVRSADSQPPELMPALSAIVKGAFYE
                     SDCLDAAWDLVKDWTWDERMQVYLDSHRDALAARIRRYSLLDLSKELLQIALEGLRRQ
                     KMFNALGEDETVYLEPLGKLLALGKCPADLLIEKWQGELDQDIRKLIAYSAYQLP"
     gene            complement(1569..2642)
                     /gene="nagZ"
                     /locus_tag="VF089_06520"
     CDS             complement(1569..2642)
                     /gene="nagZ"
                     /locus_tag="VF089_06520"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX6173651.1"
                     /translation="MEKLESDVGQMLMVGISGYELTTAELKICRSLNFGGFILFSRNC
                     CEAAQIHSLCRSLCQVTGNIPFIAIDEEGGRVHRLPPAFTHFPPAARIGKTASLDLAF
                     RVGRAVAAELTLVGINLNFAPVLDVDSNAENPIIGGRSFGTTPERVAAMALAWSEGLR
                     SMGIIPCGKHFPGHGHTDTDSHLQLPRVTKSLSELHAVELKPFVHACRSRIEGLMTAH
                     VVFPALDEEFPATLSGRIVTRVLRQELGYKGVVFSDDMEMKAITGNFDAGEAAVSSVR
                     AGIDVLLYCHDLAKAAGIVELLCREARDDSQLLARIEESHARIDVLKERGLRQHRSLP
                     DTELIERLIALDHRKLVDEIHGS"
BASE COUNT          659 a          724 c          719 g          619 t
ORIGIN      
        1 ccttctatgt cgccggaccc acttcctcga ttgatttgaa ttgcccgacg gggaaggaga
       61 ttccgatcga gcagcgcgat cccaaggaag tgtcgcacat gttcggcaga ccgctggcac
      121 ctgaaggcat gaacattcta aatcccgcat tcgatgtgac ttcacaagag ttgatttcgg
      181 cgatcatcac cgagaagggg atcatttatc cgccctatcg ccagaatatc gaaaaccatg
      241 tcagacattg agaaaaagga ccagctggaa gaatacttcc atgcggcggg caaaccccgc
      301 gagcgctggc gggtcggtac tgagtacgaa aaggttggca tcgatcgcag cagcggcaaa
      361 gccattccat attccggacc gcgcggcgtc gagtcgattc tgaagcgact gatcgatcgc
      421 ttcggctggg aaccggagga acaggatggc catattatcg cgctcagccg cgacaacgcg
      481 cagatcacat tagaacccgg cgggcagatc gagcttagcg gtgaaccttg cgacagtatt
      541 cattgcactt acgccgaatt tacgcagcac atccgggagc tactagaggt ttcggagccg
      601 ctcgacgttg ttttcctcgg attgggcatg cagccggtga gtaggttgga cgaaatcgag
      661 tgggtgccaa aaaaacgtta ccggatcatg gggccttata tgcccaaagt cggcaggctg
      721 ggccagcgca tgatgaagca gacggcgact gtccaagcca atatcgacta ccgcgacgaa
      781 aaagatgcta tggcgaagtt tcgcacgggt atggggttga cacccatctt tatctccatg
      841 ttcgccaact cgcccatctc tgatggccaa ctgaacggct accgaagcta tcgtgagcat
      901 atctggaccg ataccgataa cagccgatcc gggatgctaa agtttgcctt ctcagcggac
      961 gtttctttcg cgcactatgt cgaatatgcg ctggacgtgc cgatgtattt tattattcgc
     1021 aacaagaact acgtcgatat gacaggtacg acctttcgtc aatttttggc gtacggctgg
     1081 aacggtcagc gcgcgacctt ggacgactgg catgatcatc tgaccacgtt gtttcctgaa
     1141 acccggatca aacgatatat cgaggtgcgt tccgccgaca gccagccgcc agaattgatg
     1201 cctgcgcttt cagcgatcgt caagggtgcg ttctacgaat ccgactgttt agacgccgct
     1261 tgggatttgg tcaaggactg gacctgggat gaacgaatgc aggtgtatct cgattcgcat
     1321 cgtgacgctt tggccgcccg catccgtcgt tactcactcc tggatctgtc gaaagagttg
     1381 ttacagatcg ccttggaggg gctaagacgt cagaagatgt tcaatgcgct tggcgaggat
     1441 gagactgtct accttgaacc tctgggaaaa ctgttagccc ttgggaaatg tcccgccgat
     1501 ctgcttatag aaaaatggca gggcgagttg gatcaagaca tccgcaagtt aatcgcttac
     1561 agcgcttatc aactcccgtg aatctcatcg acgagttttc tgtggtcgag agcaatcagg
     1621 cgttcaataa gttcggtatc cggcaaactc cggtgctgcc gtaaaccgcg ttcctttaac
     1681 acatcgatgc gtgcgtgact ttcttcgatc ctagcgagta gctgactgtc gtcgcgggct
     1741 tcacggcaga gcagctcaac aatgccagcg gcttttgcca aatcatgaca ataaagaagc
     1801 acatcgatgc cggcgcgcac cgatgaaaca gccgcttcgc cggcatcgaa gtttccggta
     1861 atcgctttca tctccatgtc atcgctaaac accactcctt tatagccgag ctcttgacgc
     1921 aaaactcggg tgacaattcg gcctgacagt gttgcgggaa attcctcgtc aagcgccgga
     1981 aaaaccacat gagcagtcat caatccttcg attcggcttc tgcaggcatg gacgaacggc
     2041 tttaattcca ctgcatgcag ttcgctcagt gattttgtta cccgaggaag ctgtaagtgc
     2101 gagtctgtat cggtatggcc gtggccaggg aagtgcttgc cgcacgggat tatgcccatg
     2161 ctgcgtaagc cttcgctcca ggccagcgcc attgccgcca cccgctccgg tgttgtcccg
     2221 aaagagcggc cgccaatgat cgggttttcg gcattggaat caacatccaa gactggagca
     2281 aaattcagat tgatgccgac tagagtgagc tccgcggcaa cagcacgacc aactctaaag
     2341 gcaagatcga gactcgccgt tttgccgatg cgcgcggcag gcggaaaatg agtgaaggcc
     2401 ggaggcagac gatgaactct tccaccttct tcatcgattg cgataaacgg aatattgccg
     2461 gtcacttggc aaagcgagcg acacaaagaa tgaatctgcg ctgcttcgca gcaattgcgg
     2521 ctgaagagaa taaatccgcc aaaatttagc gaccggcaga tcttcaattc ggccgttgtc
     2581 agctcataac cgctaatacc caccatcagc atctgtccga cgtcgctctc tagtttttcc
     2641 acgatcagca actcttcaca cggtggacga tttttaatgg actgtgttat atttgcagac
     2701 ttccatgatc aataccaagt a
//
LOCUS       DASUTG010000287         8049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_342376, whole genome shotgun sequence.
ACCESSION   DASUTG010000287 DASUTG010000000
VERSION     DASUTG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342376"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VF089_06525"
     CDS             <1..527
                     /locus_tag="VF089_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6173652.1"
                     /translation="AILKKIGVMYQSSALFGSMTVLENVSLPLEEFTALPSEAIRVIA
                     LIKLKQVGLESFGNHMPSELSGGMQKRAAIARAMALDPRVLFLDEPSAGLDPITSAEL
                     DELILSLARTLKITFVIVSHELSSIFTIADRVIMLDKQSKGIVATGNPHELRDQSDNP
                     WVRQFFSRLPSSRT"
     gene            564..1622
                     /locus_tag="VF089_06530"
     CDS             564..1622
                     /locus_tag="VF089_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX6173653.1"
                     /translation="MRKLGYFKIGLFVISAILIGISGVIALGLGSIFQTKNLAETYIE
                     ESVQGLDVGSPVKFRGVSIGKVEQITLTSVEYLTRHRYVLVRFDIAPNIFGFPAGDKS
                     NKALEDELQYGFRVRLAAQGLTGVAYIEADYLDPERNPPLRIDWNPEYPYIPSAPSRI
                     TQLSESVERILHNFEELDIPQLTASVEKSLTAMTKLAEGANLQEIGRQANALLSEIRD
                     TNRQLRVLVHAPELKSSLADAAAAAGTARKLLENAEKPVNQLLADLPRVSESVSQVAL
                     RLEAVTADLPQTSAQFQQTLRQLNRLIVTQQQQIQTTLESLRLVTEDIQEFANESRRY
                     PSQILFGAPPPRSEVFEQ"
     gene            1631..2302
                     /locus_tag="VF089_06535"
     CDS             1631..2302
                     /locus_tag="VF089_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX6173654.1"
                     /translation="MGLLLTSVAASFLFSGCVSIEKSYPDKRYFVLDIAPDAPAQNPA
                     ETGILQIGSARVSPRYADKNFVYRRSDARFESDFYNEFLISPGVLVAEELRQGLSQAG
                     LFKFVVGSVNALAPTHTLEIVVNTLYGDFRDIRAPQAVMEIEFFLSQEESPSGIVFHK
                     RYHRSISLKGRNPEALVQGWNQALDAISRSLVSDLKASNLQAERRTTSRLPHFRLGWV
                     PSSVC"
     gene            complement(2446..3915)
                     /locus_tag="VF089_06540"
     CDS             complement(2446..3915)
                     /locus_tag="VF089_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6173655.1"
                     /translation="MANTIFEFDVAIIGGGGAGIPAAIEVGRAGGTAVVLEQAEECGG
                     TAAISGGGCCIVGTPLQKSQGIEDTPDLAFEDWIKWGGGAADEVWARYYLEHTLHDLY
                     YWAENCGARWVDMKFQEGNRVLRWHRPENNGLGLMTALIKTAESMPNIKIMTGTGAGK
                     LLTKNGRVVGITAIRNNETIEIFSKAVVVATGGFNSNLDMVLEVRPEFQKFRVLEGSG
                     FGAKGIGHKMIKELDGYFTHMDHIWFYVYATPDYRDPQQRRGLVFRMTPGYIWFNQQG
                     KRFHNESITGGASATPALLAQNPPHAWAIVDHPMTAKMDVADPYYRRGDVIDHAKVQE
                     LLDNSPYIRKAESLEELGKKIEVDLASFLSEIKDYNDAFDRGLETEPKFGKSLKLSKK
                     FDTPPYYAIQLFPLARKNFGGVKTDLRCRVLNKHFEPIPGLYAAGEVSGMAGGHINGR
                     NGLEGTMLGPSVFSGRVAGAWAAHEAGFGSGFVGTPNRP"
     gene            4176..5159
                     /locus_tag="VF089_06545"
     CDS             4176..5159
                     /locus_tag="VF089_06545"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173656.1"
                     /translation="MASVKARQVPWLFFALFLFAVRPAAGAENMRVAHPSLSSSVICL
                     LIANKEGYFKEEGINVEFLSIRGEIAIRTALAGEVDFFTNAGSAMAAAARNVPVKILA
                     VFQDKPGWDLIAQPNIKSIGQLRGSTIAIMSPEGSLAVVTREILKQNGMDPAKDANLV
                     VMGGDEVRLPAMKAKAIQASLFNAAASVRAQKEGFIKLAAASEYANTLQGGLATTEEK
                     IRQHPARMAKFIRGSLKGLQFFIHKRDASMKHMMEFLKTNDREMAAAIYDIEAKLIVR
                     DGASEDKVLQPMIDEIKRATKTQRDMKVSDVFDFSFVRKANEELKMSGWRP"
     gene            5391..6635
                     /locus_tag="VF089_06550"
     CDS             5391..6635
                     /locus_tag="VF089_06550"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173657.1"
                     /translation="MRFSPNPWLVAALAFIVLGFSRGLHSSFGVFNVALLDSFNWSRG
                     ATAGIFSVVLTIDAMLSPVAGYLLDHYGAKKIVIAGCLTLALGLFLSSQVSELWHLYI
                     CFGMITAFGFTFVGMVPHVFLISEWFSSNRASAIGVVYAGSGVGIMILAPLSAWLIAT
                     YGWARALEIYAAVVLISLLPLVWIFYQHGPFGEKLGHHGQKRAEQNQWTAKLALRSLQ
                     FWLLFFARVCAAAGTTVIVTHQVAHVVEVGYSQLLAASIFGFAGITSSFGRVVFGFIA
                     DRLSRQAAYTLNIAMTLIGVGALMILHDPSQSWLLYVYVIFFGIGFGSRAVIFSALTA
                     DIFSGKGFGSILGYSTVAVGVGGALGSWLGGVYYDWTGSYLVSFGLSALVLSLSDICI
                     WLAAVPSVANYDKRLWGESPTA"
     gene            6788..7162
                     /locus_tag="VF089_06555"
     CDS             6788..7162
                     /locus_tag="VF089_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6173658.1"
                     /translation="MSDPMDARLREGINLFNQRRFFEAHEIWEMLYQTAEINDKPFLE
                     GLIQLSAACRLIEDFGEIKGPVKLIYQALIRFENYQPTYLDIKIADLAAAMESWAKEI
                     EASGARGQKPIPTIAVRRFGFF"
     gene            7163..>8049
                     /locus_tag="VF089_06560"
     CDS             7163..>8049
                     /locus_tag="VF089_06560"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX6173659.1"
                     /translation="MSLSQLEETLHRFLNGRWAGAKRLQGLQGGALAYVLGLAAAENN
                     RPILVIASSAQDAENLHDDLSFFIGEEPSLAPFRRRLHLFPSWEVLPFEKLSPHPDNL
                     AGRLEGLYKLIEEPAPILISTPAALMQKVIPREALKHSYLYLVAGQDLPREVLTEHLA
                     QWGFQNVPLVEECGDFSVRGGIIDLFSPGYQLPIRLEFDGDRLESIREFNASSQRSEG
                     VLEDLLLLPVKEFSLKRLGTDQIVRKLDQRGIDLDMDRREKNALLDSVKEGIPFPGIE
                     FLLPYFYPELTSVFSYLPS"
BASE COUNT         1799 a         2115 c         2126 g         2009 t
ORIGIN      
        1 tcgctatcct caaaaagatc ggcgtcatgt atcagagcag tgcattgttc ggttcgatga
       61 cggttctaga aaacgtttct ctgccgctcg aagaattcac ggcgctgccg agtgaggcga
      121 tcagggttat cgctttgata aaacttaaac aagtcggtct ggaaagtttc ggcaaccata
      181 tgccttcaga attaagcggt ggcatgcaga aacgggcggc catcgcccgg gccatggcgc
      241 ttgatccgcg ggtgctgttt ctcgacgagc cctccgcggg tctagacccg atcacttcgg
      301 ctgaactgga tgagctgatt ttaagtctgg ccagaactct gaaaatcaca tttgtaatag
      361 tcagtcatga gctatccagt atctttacga ttgcggatcg agttattatg ttggataaac
      421 agtcaaaagg aatcgtggcg accggaaatc cacacgagct gcgcgatcag agcgataatc
      481 catgggtgcg gcaattcttc tcgcgcctgc catcttcgag gacttgagtc cagcgcaaga
      541 caggactcca actcacatca aggatgagaa aactcggata ctttaaaatc ggtttatttg
      601 taattagcgc catactgatc ggcatcagcg gtgtgatcgc tttgggtctc ggttcgattt
      661 ttcagacgaa gaatcttgcc gaaacctata tcgaggagtc agtgcaggga ttggacgttg
      721 gctcgccagt gaaattccgc ggcgtctcaa tcggtaaagt cgagcagatt accctcacca
      781 gcgttgagta tctcacccga catcgctacg tgctggtgcg ttttgatatt gcgccgaata
      841 tttttggttt tcccgccgga gataaatcca acaaggcgct ggaggatgag cttcaatacg
      901 gatttcgcgt aaggttagcc gctcagggat taacgggtgt cgcctatata gaagcggact
      961 acctggatcc cgaacgtaat ccaccgttga gaatcgattg gaaccccgag tacccgtata
     1021 ttccgtcggc tcctagcagg ataactcaat tgagcgagtc agtagaacgc atcctgcaca
     1081 atttcgagga gctcgacatc ccccaactca ctgccagcgt tgaaaaatcc ctaaccgcca
     1141 tgactaagct tgccgagggg gctaatctgc aggaaatcgg caggcaggcc aacgccctgc
     1201 tgagtgaaat tcgagacacg aaccggcagc tcagggtgct ggtgcatgca cccgagctga
     1261 agagttcctt ggcagatgcc gccgcggcag cgggcacggc gcgaaagcta ctggagaacg
     1321 ccgaaaaacc ggtaaaccag ctgctggcgg atctaccgcg cgtatcagaa agtgtcagtc
     1381 aggtggcgct gcggctggag gcagtcacgg cagatctgcc gcagacaagc gctcagtttc
     1441 agcagacact gcggcaactg aaccggctca tcgtgacaca gcagcagcaa atccaaacca
     1501 cgttggaaag tctacgattg gtaaccgaag atatccagga atttgctaat gaatctcgca
     1561 gatatccatc tcaaattctt ttcggcgcgc cgccacctcg ctcggaggtc ttcgagcaat
     1621 gataatgtgg atgggcttgt tgttgacatc cgtggcagca tcttttctct tcagcgggtg
     1681 tgtcagcatc gagaagagct atcccgataa acgttacttc gtgctcgaca tcgctcctga
     1741 tgcgccggcg caaaatccag cagaaaccgg catcctgcaa atcggatccg cacgcgtatc
     1801 gccgcgctat gcggacaaaa actttgtcta tcgcagaagc gacgctcgat tcgagtcgga
     1861 cttttacaac gaatttttga tatctcccgg cgtgcttgtc gccgaagagc tgcgccaagg
     1921 attgtcacaa gccgggcttt tcaaattcgt cgtcggctct gttaacgcat tagctccgac
     1981 gcatacgctg gaaattgtcg tgaacactct gtacggcgac tttcgcgaca tcagagcgcc
     2041 gcaagcagtg atggagatcg agttttttct cagtcaagag gaatcaccca gcggcatcgt
     2101 ctttcacaaa cgctaccata gaagtatttc tcttaaaggg cgtaacccgg aagcgctggt
     2161 gcaaggatgg aatcaggctc tcgatgcaat ctcgcggtct ctcgtcagtg atttaaaggc
     2221 gtctaatctg caggcggaaa gacgaactac ttcccgactg ccgcatttca ggctgggttg
     2281 ggtgccgagt tctgtatgct gagatcttag acacagaacg tttggcacat ctggaatgta
     2341 gaacagtgcc atgagcgatc ctgcccgatg agtctgagct tgaacgacac gaaccgaaca
     2401 atacggcgac agagtaggaa ctctccagcg gcgactcagg ggatgtcacg gccggttggg
     2461 cgtgccaaca aaccccgaac cgaaaccggc ttcatgtgcc gcccaagcac ctgctacccg
     2521 tccgctaaaa actgacggcc cgagcatcgt cccttcgagt ccattgcgcc cgttgatatg
     2581 accgcccgcc ataccggata cttcacctgc cgcatacagc ccggggatcg gctcgaagtg
     2641 tttgtttagc acgcgacagc gcaagtccgt tttgacccca ccgaaattct ttcgtgcaag
     2701 cggaaacaat tggatggcat agtagggcgg tgtgtcgaac ttctttgata gcttcaaaga
     2761 tttgccgaat tttggttcag tttctaagcc ccggtcaaag gcgtcgttgt agtctttgat
     2821 ttcagaaagg aacgacgcta aatcgacttc gattttcttg cctaactcct ccaggctttc
     2881 ggctttacga atatatgggg aattgtccag aagctcctgg actttggcat ggtcgatgac
     2941 atcgccgcga cggtaatagg gatcagcgac atccattttt gccgtcatcg gatgatccac
     3001 gatcgcccat gcgtgcggcg ggttttgcgc tagaagggcg ggagtagccg aagcgccgcc
     3061 cgtgatggac tcattatgga aacgctttcc ttgctgattg aaccatatgt accctggagt
     3121 catacggaag acgagaccgc gtctctgttg gggatcgcga taatctggtg tggcatacac
     3181 ataaaaccag atgtggtcca tgtgggtaaa atagccatct agttccttta tcattttatg
     3241 accgatacct tttgcgccga aaccagaacc ttccaacact ctgaacttct ggaattcggg
     3301 acgaacctcc aacaccatgt cgagattcga attgaaacct ccggtagcaa ccacaactgc
     3361 cttgctgaag atttctatgg tttcattgtt gcgaatcgcg gtgatgccaa ccacacggcc
     3421 atttttggtg agcagtttgc ctgctccggt acccgtcatg atcttaatgt tcggcattga
     3481 ttcggcagtt ttgatcagcg ccgtcatcaa cccgaggccg ttattttccg gccggtgcca
     3541 gcgcagcacc cgatttccct cctggaattt catatcaacc cacctggcac cgcaattctc
     3601 ggcccagtag tagaggtcat gcagcgtatg ttccagataa tatcgcgccc acacctcatc
     3661 cgccgccccg ccgccccact taatccaatc ctcaaacgcc agatccggtg tgtcttcaat
     3721 cccctgtgac ttttgtagcg gcgtgccaac gatacaacag ccgcccccgg aaatcgccgc
     3781 agtgccgcca cactcctcgg cttgctccaa gaccaccgcg gtgccgccag cgcggccaac
     3841 ctcgatcgct gcgggtattc ctgcgccgcc gccgccgata attgcaacat cgaactcaaa
     3901 tattgtattg gccatcgggg tctccttctt acgcttgcat tgcgaggatt atcaccgttt
     3961 tccgcccggg gtcaaatatc gtaaacttag gtcgattgtt tgcgatcgtt cgcatcagga
     4021 ggcggaggcc ctctacatta aggttcgcca atgaaacatt gcaggtgtaa atttttacaa
     4081 caccgaagtc caatcttcgc ttgcgaatcc tctaatcttt ctataagcga gaagaaagcc
     4141 gttccggttc atgaaggtaa ggagggattg tgacgatggc ctctgtaaaa gcacgtcagg
     4201 ttccgtggct gttttttgcc cttttccttt tcgcggtacg tcctgccgct ggcgcagaaa
     4261 atatgcgcgt tgcgcatccg tcgctttctt cgtcggtcat ttgcctgctc atcgccaaca
     4321 aagagggcta cttcaaagaa gagggcatca atgtcgagtt cctgagtata cgtggcgaga
     4381 tcgccatccg aacggcgcta gctggcgaag tggacttctt cactaatgcg ggcagcgcga
     4441 tggccgcggc cgcgcgcaat gtgccggtga aaatacttgc tgtttttcaa gacaagcctg
     4501 gttgggacct tattgcacag ccgaatatta aatccatagg tcaactgcgg ggcagtacca
     4561 ttgctataat gtcgccggaa ggttcattag cggttgtgac gcgggaaatt ctgaaacaga
     4621 acggtatgga tcccgcgaaa gatgccaatt tggtcgtcat gggtggcgac gaggttcgtc
     4681 ttcccgcgat gaaggctaaa gcgatccaag cctccttgtt caatgccgcc gccagtgttc
     4741 gggcgcaaaa agaaggattt atcaaactgg cagccgccag cgagtacgcc aacaccttgc
     4801 agggcggtct ggcgaccacc gaagagaaga tcaggcagca cccagcgcgc atggcaaagt
     4861 tcattcgggg cagtctgaag ggattgcagt tcttcatcca caagcgggat gcgtcgatga
     4921 agcacatgat ggagtttctg aaaacgaacg accgtgaaat ggctgcggcg atctatgata
     4981 tcgaagcaaa gcttattgtc cgcgacggcg ccagtgaaga caaagttttg cagccgatga
     5041 tcgatgagat aaaaagggcg acaaaaactc aacgcgacat gaaagtaagc gatgtctttg
     5101 actttagctt cgtgcgtaaa gcgaacgaag aattgaagat gagcggctgg cgcccgtaaa
     5161 ttcatatccg ttccgctaaa taggcagagg gacgaagagg ttctgaaaat tcgaatccgg
     5221 ccatgctgct gcttcgtaat ttctaacctt gctaattatt agacggcgcg ttgtgcagat
     5281 agtcggcttc tactgatcta ctcaggattt ggctatacaa tcttcggcct gcccgctaac
     5341 ctggcggctg accttccaat ttgccattta gactgggctc acctatcctc atgcgttttt
     5401 ctcccaatcc ctggttagtc gctgctctcg catttatcgt tctcgggttt tcgcgcgggc
     5461 tgcattcctc attcggagtt ttcaatgtcg cattgctcga ctccttcaac tggagccgag
     5521 gcgccactgc cggtattttc tccgttgtgc ttacgattga cgcgatgttg tcaccggttg
     5581 ccggatatct tctggaccat tatggagcaa aaaaaattgt catcgccgga tgccttactt
     5641 tggcgttggg actttttctg agcagccaag ttagcgagct ttggcacctg tatatctgtt
     5701 ttgggatgat tactgctttt ggctttacct tcgtcggcat ggtgccgcac gtctttctga
     5761 tctctgaatg gttctcctcc aatcgggcct cggctatagg cgtcgtctac gccggctccg
     5821 gcgtaggaat catgattctt gcgccgctca gcgcatggct gatcgcgacc tacggttggg
     5881 cgcgagcctt ggagatctat gcggcagtgg ttttgatttc gctcttgcct ttggtgtgga
     5941 tcttctatca gcacggacct tttggcgaaa agctcggcca tcatgggcag aaaagggccg
     6001 agcaaaacca atggacggca aagctcgcgt tgagaagcct tcaattctgg ctgcttttct
     6061 ttgcgcgagt ctgcgccgcc gcgggtacga ctgtcatcgt gactcatcaa gtcgctcacg
     6121 tcgttgaagt gggctatagc caattgctgg ctgcatccat ctttggtttt gccggtatca
     6181 cgagcagctt tggtcgggtc gttttcggct tcatcgccga tcggttatcg aggcaggctg
     6241 cctatacgct taacattgcg atgacgttaa taggtgttgg cgctctaatg atcctgcatg
     6301 atcccagcca aagctggttg ctttacgttt acgtcatctt ctttggcata ggtttcggct
     6361 cgcgggcagt cattttctcg gccttgaccg cagacatttt ttccggcaaa ggctttggtt
     6421 cgattcttgg ctactcgaca gtggccgttg gtgtcggcgg tgcgctgggc tcctggttgg
     6481 gtggtgttta ttacgattgg accggcagtt atcttgtttc gtttggctta tcggctttgg
     6541 tgctgtcctt atcagatatc tgcatctggt tggctgcagt cccatctgtc gctaattatg
     6601 acaagcggct ttggggagag agtccaacgg cctgatagcc tagccttcgc tccgcgcgcc
     6661 tgatgcattg cttacacaaa gcatccgccg cgtcgtgcta ttttcgcccg gccgcgcgcc
     6721 cctctaagtc gcgtagcgat caccggtgca tcatccttga gggtatggcg agaagttgtt
     6781 agcttgaatg tccgatccta tggatgcccg cttgcgcgaa ggtataaatc tttttaatca
     6841 gcgtcgtttt tttgaggccc atgaaatctg ggagatgctc taccaaacgg ccgagataaa
     6901 cgacaaacca tttctcgagg gattgatcca actgtccgcg gcttgccggt tgatcgaaga
     6961 tttcggcgag atcaaaggac cggtgaagct aatctatcaa gcgctgatcc gctttgagaa
     7021 ttatcaaccc acctatttgg acatcaagat cgccgatcta gccgcggcga tggagtcttg
     7081 ggccaaagag atcgaggcca gcggcgctcg gggacagaag cctatcccaa ccatcgccgt
     7141 gcggcgattc gggtttttct aaatgagcct ttcacaactg gaagaaacac tgcaccgctt
     7201 tctcaatggg cggtgggccg gagcgaagcg gcttcagggc cttcaaggcg gagcgctggc
     7261 ctatgtgttg gggctggcgg cggcagaaaa caatcgccca atactcgtca tagcctctag
     7321 cgctcaggat gctgaaaatt tgcatgacga tcttagcttc tttatcggcg aggagccctc
     7381 tttagctccg ttccgtagga ggcttcatct gttcccctcg tgggaggttc tcccttttga
     7441 aaagttatct ccgcatcctg acaatctggc tggaagatta gaaggacttt ataagctgat
     7501 cgaagagccg gcgccaatcc taatatcaac gcctgccgca ctgatgcaga aagtcattcc
     7561 gcgagaggcg ctaaagcatt cttacctcta ccttgttgct ggtcaggatc tgccccgcga
     7621 agtcttaacc gagcatctgg cgcaatgggg ctttcaaaac gtacctttgg tcgaagagtg
     7681 cggcgacttt agcgtccgtg gcggcatcat cgatctcttt tctcccggtt atcagttgcc
     7741 gattcgtctg gaattcgatg gtgaccgttt ggaatcgatc cgggaattta acgcctccag
     7801 tcaacgctcg gaaggagtct tagaagatct tctgctgttg ccggtgaagg aattttctct
     7861 caagcgactc ggcaccgatc aaatcgtcag gaaactggac cagcgcggga ttgacctgga
     7921 tatggatcgg cgcgagaaaa acgccttgct ggattcggtc aaggaaggca ttccttttcc
     7981 cggcatcgaa ttcttactgc cctatttcta tcccgaactg acatcggtgt tttcttatct
     8041 gccatccga
//
LOCUS       DASUTG010000288         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1231379, whole genome shotgun sequence.
ACCESSION   DASUTG010000288 DASUTG010000000
VERSION     DASUTG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231379"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VF089_06565"
     CDS             <1..609
                     /locus_tag="VF089_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173660.1"
                     /translation="PGVRRIGSPSIQMFLDDEAVFLFDWTEQILSEASVHEGNMSPPP
                     YIQEHPELKEELFRQGAIAGEWLHAQGYRGTASTDFVVIEQDGRNKVIICEINARVTG
                     ATYPAVLARHFMPAGCWKMRNIQFRKSLDGDQLLSLIDNAQVLYRPGKDKGIIPFNLN
                     TDDQGKVIKGQFLCLGEDHEQCSGVLAKAWTELPVEWGYDRD"
     gene            596..1753
                     /locus_tag="VF089_06570"
     CDS             596..1753
                     /locus_tag="VF089_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6173661.1"
                     /translation="MTETSRKDESLRRRLIELTRDLIIIPSTRDRPEDIERCMEFVIN
                     HVELPHELTVRRFNEKGSPSTVIQPHDVSAPEVLLLAHIDVVARPEGAEYRSSVNDGR
                     IYGPGSGDMKGELAVLLETFRDFHERLPGVSLGLAVTSDEENGGDHGTRFLFEKTGLR
                     CGVVIIPDSGSLNEIAVEEKGILHLKLKAYGSSGHASRPWLSENPLEKLISKLNEVRN
                     YFETLKNGDGHWHPTIAVTVMQTENRVSNRIPSLAEAVCDVRFPPPYKSNEVISQLER
                     FMDPKMALEVLVSAEPTKVFPDPLFLAATEEITQKPVTLIREHGGSDARFICGYGIPV
                     IMSRPLVGNLHAENEWIDIASMETLYHIYQRYLEKKLNNGATSSSSQNNIA"
     gene            1922..3172
                     /locus_tag="VF089_06575"
     CDS             1922..3172
                     /locus_tag="VF089_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173662.1"
                     /translation="MIGLVSAIRIVGGGLHQFGFTVFFLPISQDLGISRAATSLAFSL
                     SRAQGAIEAPLVGYLIDRYGPRPIMVTAVFLAGIGYILLSWTDSYAGFMIVYLGVISL
                     AFVAGFVHSPMVVANSWFIRQRARAMTVVSAAVPIGGALISPLLAIGVSSIGWRWAAF
                     LSGCVFLLVCLPLSFQLKRSPESMGLLPDGDPTTLNEGVDLSPKTVDINVKPDFTSRQ
                     AMKTWMFWVLVTAMTARVTCYSAATVHFIPLMVWKGMSEGAAASLLGVFALVNLLAHF
                     VLGWIADRVNKPKLLAACHLLPALSVVPLLFESAHYQLWLFAIGFTLLDASFPIVWAT
                     VGDFFGRRHFATIRGMMSFFYMWGSFAGPVLAGVIYDTTQAYWMVLWIFLSLLIIATL
                     LVLSLIRPWTIRMIATESSSRRAR"
BASE COUNT          762 a          961 c          937 g          919 t
ORIGIN      
        1 cctggagtcc ggcgcatcgg ctctcccagc attcagatgt ttttagatga tgaggctgtt
       61 tttttattcg actggaccga acagattctc agcgaggcca gcgttcatga aggcaacatg
      121 tcgccgcctc cctatattca agaacatcct gagctcaagg aagagctttt ccgacagggc
      181 gcaattgccg gagaatggct gcacgcccag ggctatcgcg gtacggccag taccgacttt
      241 gtggtcatag aacaagacgg acgtaataag gtcatcatct gcgagatcaa tgcacgggtg
      301 acgggcgcaa cctatcccgc agtgctggcg cgacacttta tgcctgccgg atgttggaaa
      361 atgcgaaaca ttcagttccg caaatcactc gatggcgatc agcttctctc cctcatcgat
      421 aacgcacaag tgctctaccg gccgggtaag gacaaaggaa tcattccttt taatcttaac
      481 actgatgacc aaggcaaagt catcaaaggt cagtttctct gccttggaga agaccacgaa
      541 caatgcagcg gggtattggc gaaagcctgg accgaattgc ctgtcgagtg gggttatgac
      601 cgagactagc cgtaaagacg aatctctgcg ccggcggttg atcgagctca ctcgcgatct
      661 gatcatcatt ccgagcacac gcgatcgtcc cgaagatatc gaacgttgca tggaattcgt
      721 catcaatcac gtcgaactgc cccacgaact gacagttcgg cgttttaatg aaaagggaag
      781 tccgtccacg gtgatccagc cgcacgatgt ttcagcacct gaggttctgc ttcttgccca
      841 cattgacgtc gttgcacggc cggaaggcgc ggagtaccgc tcgtcagtca atgatgggag
      901 aatttatgga cccggcagcg gcgacatgaa aggagaacta gcagtactgc tggagacttt
      961 ccgtgacttc catgagcgct tgcccggagt ttcactaggc ctcgcggtaa cctcggatga
     1021 ggaaaacggc ggggatcatg ggacacgatt tcttttcgag aaaaccgggc tccggtgcgg
     1081 cgttgtgatt attccggact cgggctctct caacgaaata gccgtcgaag agaagggcat
     1141 tctccacttg aagctcaagg cgtatggatc ctcgggccat gcttcgcggc cttggctctc
     1201 ggaaaatcct ttagagaaat taatatccaa gttgaacgag gtgcgaaact attttgagac
     1261 tctcaaaaac ggggatgggc attggcatcc gaccattgca gtcactgtga tgcagactga
     1321 gaatcgggta tcgaaccgca ttccatcgct tgcagaagcg gtctgcgatg ttcgattccc
     1381 gcctccgtat aagtcgaacg aagtgatttc tcagttagaa cgattcatgg atcctaaaat
     1441 ggcgctcgaa gtgcttgtca gcgccgagcc aactaaggtc tttcccgatc cgctctttct
     1501 tgcggcgacc gaagaaatca cgcagaaacc agtaacactg atccgcgagc acggcggaag
     1561 cgatgcccgc ttcatctgcg gttatggcat tccagtcatc atgtctcggc cgctggtggg
     1621 caatctacat gccgagaatg agtggatcga tatcgcctcc atggagacgc tctaccatat
     1681 ttaccagcgt tacttggaaa agaagctgaa taacggggcc actagcagtt cttcgcagaa
     1741 caacatcgct tgagcggttt gtctttccgc tgttcttcaa tcgaaacgcc gttaaggtat
     1801 ttcaatctcc aggaaaaccg ctagccccaa ccattaggtc ctttgtttta ccaccgactc
     1861 cagccacatg aacttcaata gctttcgcaa actgttctcg ggcttatatt acggttggca
     1921 tatgatcgga cttgtgtcgg ctatccgcat cgttggcggt ggtctgcacc aattcggctt
     1981 cacggttttt tttctgccaa ttagccaaga tctaggaatc agccgggcgg caacgtcgct
     2041 ggcgttttcg ctttcgcggg cgcagggcgc catagaagcg ccgctcgtcg gctacctcat
     2101 tgaccgttat ggcccccgtc cgatcatggt cactgcggtc tttctcgccg gcatcggtta
     2161 catccttctg tcctggaccg atagttatgc cggcttcatg atcgtctacc tcggcgtgat
     2221 ctctcttgcg ttcgttgccg gctttgtcca ctcgccaatg gtcgtcgcca acagctggtt
     2281 cattcgccag cgggcgcgtg ccatgaccgt agtcagcgcc gcagtaccca tcggcggcgc
     2341 gctgatatct ccgctgttgg ctatcggagt atcgtccatt ggctggcgct gggcagcttt
     2401 tctctccggc tgtgtgtttc tactagtctg cttgcctctt agctttcagt tgaagcgttc
     2461 ccctgaaagc atgggattgc tcccggatgg agatcccacg accctgaatg agggcgtgga
     2521 tttatcgcca aaaacagtag acataaatgt caagcccgat ttcaccagcc ggcaagcgat
     2581 gaaaacctgg atgttctggg tgcttgtaac tgcgatgact gctcgagtga cgtgttacag
     2641 cgcggctacc gttcatttta ttccgctcat ggtttggaag ggaatgtcag agggcgccgc
     2701 cgcatccctg cttggcgttt ttgcgctagt taatctattg gcccactttg ttctcggctg
     2761 gattgcagat cgggtaaaca agcccaagct tttagccgcc tgccacttgc tgcctgctct
     2821 gtctgtggtg ccgcttctat tcgaatcggc tcattatcaa ctttggttgt ttgcgatagg
     2881 gtttactctg ctcgatgcct cattccccat cgtctgggca actgtcggag attttttcgg
     2941 tcggcgccac ttcgcaacca tcaggggaat gatgagcttt ttctatatgt ggggaagctt
     3001 cgccggcccg gtcctggcgg gcgtgatcta cgatacgact caagcgtact ggatggtctt
     3061 atggattttt ttgagtttgt tgatcattgc gactttgcta gtgttgtcat taatccgccc
     3121 ctggacaatt cgcatgatcg cgacagaatc ttcgtctcgt cgagcccgat gactgcttag
     3181 ccgcgagcta accagaactc cagctcttcc caatgggagg tagtcaagcg gatgctgatt
     3241 tcctggccat gtctgagcct attgccgcat taagcaaaga ccagcaatcg cacgcgaaga
     3301 tatccgaata gcccgggtcg cgctacgcct ggtaattatt ctcccccagg gcgggacggc
     3361 ggttgatccg ggcaattaga tgatggctct cgaggagagc ctatcttgac tgctcgagaa
     3421 aagtttcgtt ctcctcgcgc agaacccggc ttttctcgat catcctgcga ccctgttcga
     3481 ccgtatttct tagttggtaa gccattgttc tttgtccctt aatacttccg attcgcgttc
     3541 gtgctcgaat aaccatttag cgagatacct tcctttcgt
//
LOCUS       DASUTG010000289         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_787028, whole genome shotgun sequence.
ACCESSION   DASUTG010000289 DASUTG010000000
VERSION     DASUTG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787028"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VF089_06580"
     CDS             complement(<1..1096)
                     /locus_tag="VF089_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173663.1"
                     /translation="MSLPASKLIKILVWLAILSPTQVLSSFAQAEPESSTSSELPSIE
                     ALKLCLAQADSAACLDKIFREVLKTESTKTALKLIQSFELQDPELRRDCHPIVHAIGR
                     ETFRIKGNIHDSFSACDQTCHSGCYHGSVERFLRGEEIYSQVERHPSQAELKEKAAAA
                     CDSDVPVRLRFQCLHGLGHALMFFSRYKLTPSLEACDALTEEWSRQSCYGGVFMENVS
                     SSTPELRDLSPTDYHYPCNRIDTRYRAECYVMQTSRMTEMGLSVSRIFEECQKSGEYD
                     LPCTLSIGRDLSNDVRIGQTRPTAEKCEAVEGSRRLACMRGVIYALIDNTWDGRYVLP
                     FCVAFEEERDQQSCIRESIGYLKTTFQKSIE"
     gene            1342..2142
                     /locus_tag="VF089_06585"
     CDS             1342..2142
                     /locus_tag="VF089_06585"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX6173664.1"
                     /translation="MTAAHAQEAVKVPVSVPLYQPKYYPYESGEKASYRASWNGVPVA
                     TAEIETTPITFEGKKSYRVRIDAKTSRGLDLIWRMRDTITSVFDAKSLAPSRFVFSQR
                     ENSRVIDTEAKFNQKAKKWAINRWQEGRKPRVYDFDSNNTLDPITAVYLARSIDLKVG
                     EKLFFNVFGGRYQYLLELNVEKKEPVELESGAVVQAFKLVPKLTNLTKKGYAGRMKEA
                     AIWVSADERRIPVKFWSRIFAGNVYMELIQDGTGVQSATTVDSPSPAS"
     gene            2175..2873
                     /locus_tag="VF089_06590"
     CDS             2175..2873
                     /locus_tag="VF089_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6173665.1"
                     /translation="MKAARTSRGGFGLFVISLGILSTVFSGFRYSPVLRDRGGSAIPY
                     SDSSRRGDASGEDRLRRTIVPLIRAMDRPCQSDELRVQIMNEPTINAANAGGCQFFVT
                     SGLLQRANDDHLLGVMAHEIAHQDLGHAVKAQMLGAGLSIGAALLEQILPGSGAIAPL
                     AGTLIARGHSRTEEYAADRHAIVLLRRIGYPPDVMIDTLSWIQRSSGQQHAGGFLSTH
                     PALNDRISALKKIR"
BASE COUNT          702 a          803 c          837 g          713 t
ORIGIN      
        1 cctcaattga tttttgaaaa gtcgttttta gatagccgat gctttctctg atacaactct
       61 gttgatcgcg ctcctcctcg aaggcaacgc aaaacggcag cacataccgg ccatcccagg
      121 tattgtcgat caaagcatag atcacgccgc gcatgcacgc cagcctgcgt gaaccttcaa
      181 ccgcttcgca tttttccgcg gtggggcgag tctggccgat tcgaacatca tttgagaggt
      241 cgcggccgat cgagagcgtg cacggcaaat cgtattcgcc gctcttctga cactcctcga
      301 agattctcga gacggagagc cccatttcag tcatcctgga ggtttgcatc acgtagcatt
      361 cggcgcggta tcgggtatcg atccgattgc aaggatagtg gtaatcggtg gggctcagat
      421 cgcgcagctc cggtgtggaa ctggaaacgt tttccatgaa caccccgccg tagcacgact
      481 ggcggctcca ttcttccgtc agcgcgtcac atgcttcgag cgagggcgtg agcttatagc
      541 gcgaaaagaa catgagagca tggcccaaac catgtaggca ttgaaagcgc aatcttaccg
      601 gcacgtcaga gtcgcaggcg gccgcggctt tctctttgag ctccgcctgg cttggatggc
      661 gctcgacttg cgaatagatc tcctctcccc gtagaaaacg ctcgaccgat ccatggtagc
      721 acccggaatg acaggtttga tcgcacgcgg agaaggagtc gtgtatgttt cctttgattc
      781 tgaaggtctc acggccgatg gcgtggacga taggatggca atcccggcgc agctcaggat
      841 cctggagttc aaagctctgg atgagcttta atgcagtttt cgtcgactct gtttttaaca
      901 cctcgcggaa aatcttgtcc aggcaagcag cgctgtcggc ttgggcaagg caaagtttta
      961 gcgcctcgat ggaaggtaac tcagacgagg ttgaggattc cggctcggct tgagcaaagc
     1021 tcgacaaaac ttgggtaggg gataagatgg ccaaccacac caagatcttt attaacttcg
     1081 atgcaggtaa tgacacaatt acgactctcc ttcttgctgc gcgactcgtg cgccggattt
     1141 tagccacaag gatcaggctg cagtaaaagc aattcggtca tcatggagca ctcagagtct
     1201 tctggcgaga ttgcaacctt gcttttcttt ccggtgaaat ctgtaactat tttacctatg
     1261 atccgtctaa agaataaaat gacccggcgg ttgcttaaac tcccttctac tattaaggcg
     1321 ttggctttag caacgctttt catgacagcg gcccatgccc aggaagcggt gaaggtgccc
     1381 gtaagcgtac cgctgtacca gccgaaatac tatccctatg agagcggcga gaaggcatcg
     1441 taccgggcga gttggaacgg cgttccggtg gccaccgcgg agatcgaaac gacacccatc
     1501 accttcgaag gaaaaaagtc ttatcgggta cgcatcgatg ccaaaacctc cagaggtttg
     1561 gacctgattt ggagaatgcg cgacaccatc acttcggtgt tcgatgcgaa atcgctggcg
     1621 ccgtcgcggt tcgttttcag ccagcgcgaa aattctcgcg tgatcgatac cgaggccaag
     1681 ttcaaccaga aggcgaagaa atgggcgatc aatcggtggc aagagggcag gaagcccaga
     1741 gtttacgatt ttgactctaa caataccttg gacccgatca cagccgtcta ccttgcgcgt
     1801 agcattgatt taaaggttgg tgagaaattg tttttcaatg tttttggcgg ccggtatcag
     1861 tacttgctcg agctgaatgt ggaaaagaag gagccggtgg agctggaatc cggcgccgtc
     1921 gtccaagcat ttaaacttgt gccgaagtta accaatttga caaagaaagg ttatgcgggg
     1981 cgcatgaagg aagcggctat ctgggtctct gccgacgagc ggcgcatccc ggttaagttt
     2041 tggagcagaa tatttgccgg caatgtttat atggaattga ttcaggatgg aactggcgtg
     2101 cagtcggcca ccactgtgga ttcacctagc ccggcgagtt aaccggtggg tatgacgtca
     2161 tctgaaacga taaaatgaaa gcggcaagaa cgtcaagagg cggatttggc ttgtttgtga
     2221 tttcccttgg aatcctgagc acagtgtttt caggtttcag gtactcgcct gtgctccgtg
     2281 atcgcggcgg ttcggcaatc ccgtattcgg actctagccg gcgaggcgat gcgtcgggcg
     2341 aagatcgtct tcggcggacg atagttccgc tcatacgagc catggaccga ccgtgccaat
     2401 ctgacgaact acgcgtgcag atcatgaacg agccaacaat caatgctgcc aatgccggcg
     2461 gttgccaatt cttcgtaacc agcgggttgc tgcaacgggc caacgatgat catttgttgg
     2521 gagtgatggc gcatgaaatc gcgcatcaag atttaggcca cgccgttaaa gcgcaaatgc
     2581 tcggcgccgg tttaagtatc ggtgcagctc ttctcgaaca gattcttccg ggctcgggcg
     2641 cgattgcgcc gctggccgga acactgatcg cccgcggtca cagtcggacc gaggaatatg
     2701 ccgccgaccg ccatgccatc gtgctgctcc gccgcatcgg ttatccgccg gatgttatga
     2761 tcgacacgtt aagctggatc caacgctcct cgggccaaca acacgccggc ggttttcttt
     2821 cgacccaccc tgcgttgaat gacagaattt cggcgctcaa gaaaattcgc tagcaggctg
     2881 atgaaaaaca cccatatgct tcgttgccac tcaatcgcct cgctgcaacg tactgttcgt
     2941 acgcctccgc gtgtcgactt tcgcgcgtct tgcctctgga tctttttgag cagcctgcaa
     3001 gaggcttttt ccagcaccct actagacgct aacagttgtg ccaaaagcca ccatt
//
LOCUS       DASUTG010000290         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1381029, whole genome shotgun sequence.
ACCESSION   DASUTG010000290 DASUTG010000000
VERSION     DASUTG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381029"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..946
                     /locus_tag="VF089_06595"
     CDS             167..946
                     /locus_tag="VF089_06595"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6173666.1"
                     /translation="MKDLAGKIVLVTGAARGIGKAIALTFGAEQSRVIICGRTAAALE
                     ELAAMLRSTGVEVLARQCDVTQKPQVESLRQEIEKSFGAVQILVNNVGIAPAADFLDM
                     PDSLWDEVMRVNVSATYNCCKTFLAPMVGARWGRIINIASTVARVTYPQISAYATSKH
                     AILGLTRSLAVETARSGITVNAVCPGYVNTELTRENARRLAEKRGRSLDEALAMLAAS
                     CPQRRLIEPEEVAYVTLMLASDRARGITGQAIQVDGGAVMA"
     gene            977..1771
                     /locus_tag="VF089_06600"
     CDS             977..1771
                     /locus_tag="VF089_06600"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX6173667.1"
                     /translation="MSYSSFVFENNDGVATIRLNDPERLNALTFQTYADLEKITAELA
                     HDPTVKVVVLTGTGKGFCSGGRVDDIIGPLLKMKGDELYKFTRMTCNVVKNMRSLKKP
                     IIAAVNGIAAGAGAMLMLASDLRIFSDKARAAFLFVKVGLSGADMGALYLLPRIVGLG
                     KATELVYFGDTIDAHEAQRIGLANRVVPGDTLMDEAYKWANRLKDGPLYALGVTKELL
                     EYEANVDLETALEMEAMAQARCMETPDFIEGYNAFTEKRPLRFNRS"
     gene            1966..2415
                     /locus_tag="VF089_06605"
     CDS             1966..2415
                     /locus_tag="VF089_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6173668.1"
                     /translation="MKKEIYVRNLEKRDLPAIVNLEERQTGIARPAYWEKRIEISEAI
                     RPHWASLVAEIDNRVVGFVLGRAGEFEFGLPGTIAWIEIIGVDPAYRGQGIAQQLVGQ
                     FAESAEDHGIKTIFTLISNSQDDMQQFFSRLGFVHGKMLHYQKELTG"
     gene            2425..3585
                     /locus_tag="VF089_06610"
     CDS             2425..3585
                     /locus_tag="VF089_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6173669.1"
                     /translation="MEFLDDDQSRSLRQKVHSWTRKNPLDVESSSETLERDACELAQR
                     LGREGLIAYAIPRPYGGMREIVQARDLCVIREELASHAALADVMFGVQALGSYPIVIA
                     GTEEQKKKYLPALASAESLAAFALTEPEAGSDAAAIGTEARRVSGGYRLHGVKRFISN
                     AGIAQIYLVFASTTPDAKAKGISAFIVPADAPGITVKEKTRLLSPHPIGVLGFDDCFV
                     PQQQRLGNEGDGLSIAYRTLDTLRSTVGAAAVGFARRALAEAISYSQRRRQFGRPIGE
                     FQGIQFKIAEMAADLEAARLLVYQAAWAHDMHDPELPLKSSIAKLFATEAAQRIVDQA
                     LQIHGGNGVVAGHIMERLYRDVRALRIYEGSSEIQKLVIAKNLIHKERPHGF"
     gene            3575..4726
                     /gene="acd"
                     /locus_tag="VF089_06615"
     CDS             3575..4726
                     /gene="acd"
                     /locus_tag="VF089_06615"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HEX6173670.1"
                     /translation="MDFELPEELAEVQKLAHDFAEKEIAPSAAQDDREHRFRKDLVGK
                     MGELGFYGCLIPEEFGGNGLGFLAHTLISEEIARVHSAIRVYLNMQAGPAVTLFEFGN
                     EEQKKKYLPGLLSGQSIGLFAITEPDAGSDVASMKTSAKRDGRHYLLNGTKIWITNAT
                     VADAGVIFAYTDRSQKHRGISAFYADLNQPGLSRRDLDKLGAHASPTGELTFDNFAIP
                     AESLIGHEGDGFKICMRHLNHTRLGCAAGAIGLAKAAREAAVNYANQREQFGEKIGQF
                     QMNQDLIAQMIVQEEAARLLVYRAASLADRGRPYNLEVSIAKYAAAEAAAFCADTALK
                     ILGAYGYSTEFPVERYYRDAKSYQIVEGSVNIQKLIIAQDALGYRKANR"
     gene            4750..5553
                     /locus_tag="VF089_06620"
     CDS             4750..5553
                     /locus_tag="VF089_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6173671.1"
                     /translation="MTRTSSKSTLKKVSLQPGIIYGPVQSRRLGRSLGINLLPAEYKL
                     CSFNCLYCQYGWTKNVTFTPGDRLKDLPSPGVVATALETALAEFSRGHKAIDAISICG
                     NGEPTLYPALAEVNVEIKKLRDRYRPDACTAILSNSSTVGDPAVRVALELLDFKIMKF
                     DAGSEEMFRQLNHPAAPVYMGEMISGLKELKNLLLQACFVQGRVTNADPDSVAMWLEK
                     LREIHPLGVQVYTLDREPADKRIERVSPTTLRWIADAVRWRAGLKVEIF"
BASE COUNT         1299 a         1536 c         1623 g         1149 t
ORIGIN      
        1 acaagcatcg aagatgctga tcaatcggag ccaggaattg gacttagcga ccgggctcga
       61 attcgaagcg cgcactttcg ctgctatcgc gcacacgcac gatctggctg agggaacgag
      121 agcgtttttg gagaagcgga aggcaaattt taccggcaat tagcagatga aagatctcgc
      181 tggaaaaatc gtccttgtca ccggcgcggc acgcggcatc ggcaaagcaa ttgccctgac
      241 gtttggcgct gagcaaagcc gggtgatcat ctgtggcaga accgcagccg cgcttgaaga
      301 actcgcggca atgctacgct ccacaggcgt tgaagtattg gcgcgccaat gcgatgtgac
      361 tcaaaagccg caggtggaaa gtcttcggca ggagatcgaa aaaagtttcg gcgcggtgca
      421 gattctggtc aataacgtgg gcatcgcgcc agcggcggat tttttggata tgcccgatag
      481 cctgtgggac gaagtcatgc gggtcaatgt aagcgccacc tacaactgct gcaaaacctt
      541 cttggcaccc atggtcggcg cccgctgggg ccgcatcatc aacatcgcct cgaccgtggc
      601 acgggtcacg tacccacaaa tttccgcata tgcgacatcc aagcatgcga tccttggctt
      661 gacccgttcg ctcgcggtag aaaccgccag gtcgggtatc accgtgaacg cggtatgccc
      721 cggttacgtc aacaccgaat taacccgtga aaacgcgcgg cggctggccg aaaagcgggg
      781 ccggtcgctg gacgaagcgc tcgccatgct ggcggcgagc tgtccgcaac ggcgtctcat
      841 cgagccggaa gaagttgctt atgtgacctt gatgctggcg tcggatcgcg cccgaggcat
      901 caccggccag gcgatccaag tcgacggcgg cgccgtcatg gcttgaacag aaacgacaac
      961 tgcaaggaga ccttggatga gttattccag ttttgttttc gagaacaacg atggcgtcgc
     1021 gacaatccgg ctgaacgatc cggaaaggct caatgcgctc acttttcaga cgtacgccga
     1081 cctggaaaaa atcacggcgg aattggccca tgaccctacg gtcaaggtcg ttgtgctcac
     1141 gggaactggc aaaggttttt gttccggtgg tcgagtcgac gacatcatcg ggcccctgct
     1201 caaaatgaaa ggcgacgagc tttataaatt cacccggatg acctgcaatg tggtcaagaa
     1261 catgcgcagc ttgaaaaagc cgatcatcgc cgcagtcaac ggcattgccg ccggcgctgg
     1321 cgcgatgctc atgctggcgt cggatctgcg catcttctcg gacaaagcgc gggcggcgtt
     1381 tctattcgtg aaagtcggtt tatcgggcgc cgatatgggc gcgctctatt tgctgccgag
     1441 aatcgtcggc ttgggaaaag ccacggagct ggtctacttc ggcgacacca tcgacgccca
     1501 cgaagcgcag cgcatcggct tggccaaccg ggtcgtgccc ggtgacactt tgatggatga
     1561 agcctacaaa tgggctaacc ggctaaagga tgggccactc tatgcgctgg gtgtcaccaa
     1621 ggagttgctc gaatacgaag caaacgtgga tttagaaacc gcgttggaaa tggaagccat
     1681 ggcgcaggcg cggtgtatgg aaacaccgga ttttatcgag ggctataatg cgttcactga
     1741 aaaacgcccg ctacgattta accgcagctg acaccatgcg cgcagagcta ggaagaacag
     1801 atgaaaagca aaccttaccc cgaaggtaac caagggcatc gagggacaag gatgagaaat
     1861 ccatgtccga aacgttgccg gcttcgatgg ttcgaggtga aatgtatttt cgctgggcca
     1921 agttaaaaat ttcagcgggg taaacacagg ggttggaggg cttatatgaa aaaagagatt
     1981 tacgtcagaa atctggagaa gcgcgatctg cctgcgatcg tcaacctaga ggagcgccag
     2041 accgggatag cgcgtcccgc atactgggaa aagagaattg aaatctccga ggccattcgg
     2101 ccccactggg cctctttggt cgccgagatt gacaatcggg tcgtcggctt tgtgttgggg
     2161 cgcgccggtg aatttgagtt cggattaccc ggcaccatag cgtggattga aataatcgga
     2221 gttgacccag cgtatcgcgg ccaggggatc gcccagcaac tcgtcggcca attcgccgaa
     2281 tcggctgaag accacggcat caagaccatt tttaccctga tcagcaacag ccaggacgac
     2341 atgcagcagt ttttcagcag actgggtttc gttcacggca agatgctgca ctatcaaaag
     2401 gagcttaccg gttaaacagc gattatggaa tttctagacg atgaccaatc ccggagtctg
     2461 cgccaaaaag tccattcctg gacgcgcaaa aatccgcttg atgtcgagtc cagttcagag
     2521 acgcttgagc gcgacgcttg cgaactggcc caacgactcg gccgagaggg cttgatcgca
     2581 tacgcgattc cccgtcccta cgggggcatg cgcgaaatcg ttcaggcgcg cgatctgtgt
     2641 gtgatccgcg aagaattggc ttcgcatgca gccctagccg acgtcatgtt cggtgttcag
     2701 gctctcggca gctaccctat tgtcatcgcc ggcacggaag aacaaaagaa aaaatacttg
     2761 ccggctttag cgagcgccga aagcctcgct gccttcgcct tgaccgaacc cgaagcggga
     2821 tcagacgctg cggcgattgg caccgaggcc cgcagagtca gcggcggtta ccgcttgcac
     2881 ggagtcaagc gattcatctc gaatgcgggg atcgctcaga tttacctcgt tttcgcgtca
     2941 accacaccag acgccaaggc caaaggcatc agcgcgttta ttgtaccggc cgatgctccc
     3001 ggcatcacgg tcaaggaaaa aacccgattg ctttcacctc atccgatcgg cgtcttgggc
     3061 tttgacgact gttttgtgcc gcaacagcaa cggctcggca acgagggcga tgggctcagt
     3121 atcgcttacc gcactctaga cactttgcgt tcgacggtcg gcgccgctgc cgtgggcttc
     3181 gcccgcagag cgttggccga agcgatctct tatagccagc ggcggcggca attcggccga
     3241 cccatcggcg agtttcaagg gattcaattt aagatcgccg agatggcggc agatttggag
     3301 gcggcacgcc tgctcgtcta ccaagccgcc tgggcccatg acatgcacga cccggagctg
     3361 ccgctcaagt catcgatcgc caagctcttt gccacagaag cggcccagag aatcgtcgat
     3421 caagcactgc agatccacgg cggtaacggc gtggtggcgg ggcatatcat ggagcggctt
     3481 taccgtgacg tgcgcgctct gcgcatttac gaaggatctt ccgagataca aaaattagtg
     3541 atcgccaaaa acctgatcca taaggagcgt cctcatggat tttgagttgc cggaagaact
     3601 cgcggaggtg caaaaactcg cccacgactt cgcggagaag gaaatcgcgc cgagcgccgc
     3661 gcaagatgac cgggaacatc gctttcgcaa agacttggtc ggcaaaatgg gcgagctggg
     3721 tttctacggt tgcttgattc cagaggagtt cggcggcaac ggtcttggat ttctcgccca
     3781 cacgctgatt tcggaagaaa ttgcgcgcgt ccacagcgct atccgggtgt atctcaatat
     3841 gcaggccggt cccgccgtga ccctattcga gttcggcaat gaagagcaaa aaaagaaata
     3901 tctgccgggt ctgttgagcg gccaatccat tggcttgttt gctattaccg aacccgacgc
     3961 cggatcggat gtggcgtcga tgaagaccag cgccaagcgt gacggtcgac attatctgct
     4021 caacggcacc aaaatctgga tcaccaatgc cacagtggct gatgcaggag tcatcttcgc
     4081 ctataccgat cgctcgcaaa aacatcgcgg catatccgct ttctacgccg acttgaatca
     4141 gccgggatta agccgtcgcg atctcgacaa gctgggcgcg catgcttcgc cgaccgggga
     4201 attgaccttc gataactttg cgattccggc ggaaagtctc atcggccacg aaggcgatgg
     4261 ttttaaaata tgcatgcgtc acttgaatca cacgcgtctc ggttgcgccg ccggcgccat
     4321 cggactggcc aaggccgccc gcgaggccgc ggtaaattat gccaatcagc gcgagcagtt
     4381 tggtgagaag atcggtcagt ttcaaatgaa tcaagatctg atcgcgcaga tgatcgtcca
     4441 ggaagaagcc gcccgtctgc tggtttaccg cgccgcctct ctggccgacc gtggccgacc
     4501 ttataatctg gaagtttcca tcgccaagta tgcggcggcc gaagccgcgg ctttttgcgc
     4561 cgacaccgcg ctcaagattc ttggcgcgta tggatactca acggaatttc ccgtggagcg
     4621 atattatcgc gacgccaagt cttatcagat cgtcgaaggt tccgtgaaca ttcagaagct
     4681 catcatcgcg caagatgcgc tgggttaccg aaaggcgaac cgctaagcgg cagatcgttg
     4741 atagtcgaca tgacccgtac gagttcaaaa tcgacgctga aaaaagtgtc cctacagccc
     4801 gggatcatct atggtcccgt gcagtcccgc cggttgggta gatcccttgg tatcaatttg
     4861 ttgcccgcgg agtacaaact gtgttcgttc aattgtcttt actgccaata tggctggaca
     4921 aagaacgtaa ccttcacgcc gggcgatcga ttgaaggact taccctcgcc cggtgtcgtg
     4981 gcaacagcgc tggaaaccgc cctggcggag ttttcccgcg gccataaagc gatcgatgcc
     5041 atctcgatct gcggcaacgg cgagccaact ctctatccgg cgcttgccga ggtcaacgtt
     5101 gagataaaaa agctccgtga ccgttaccgg cccgatgcgt gcaccgcgat tctttcgaac
     5161 tcttccactg tcggagatcc cgccgttcgc gtcgcgctcg aactgctcga ttttaaaatt
     5221 atgaaattcg atgccggcag cgaggagatg tttcgccagc tcaatcatcc ggcggcgccg
     5281 gtttacatgg gcgaaatgat ctctgggctg aaagaactca agaatctctt gctgcaggcc
     5341 tgcttcgtgc aggggcgggt caccaatgcc gatccggatt cggttgcgat gtggctcgag
     5401 aaactccggg aaattcatcc gctgggagtt caagtctaca cgttggatcg tgaacccgct
     5461 gataagagaa tcgaaagagt cagtcccacc actctgcgtt ggatcgctga cgcggtacgc
     5521 tggcgagccg gccttaaagt ggaaatattc taagcggcga aaccttggtg atgcagtcat
     5581 aggaaccggg attgtggtga cgggcat
//
LOCUS       DASUTG010000291         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1381057, whole genome shotgun sequence.
ACCESSION   DASUTG010000291 DASUTG010000000
VERSION     DASUTG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381057"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VF089_06625"
     CDS             complement(<1..1270)
                     /locus_tag="VF089_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6173672.1"
                     /translation="MLKTTWLRLGLRSQGEKPADATRTDSLADLASQIIGLFRNDLTY
                     KDTETSPAENRNALIALQWFVAVGTSYLILSSQAWGLDAPLPLLLIFLCIISAPVLQR
                     LPLKFFAGNNIEAKLLTFDSILILAAIGLTQSAPWDLLALFFFCVFIAATGENLLHIV
                     VGCTLLSLIFLIFVSSQQIDLSAINSDLLLRVPFMLGISVLYGHLTNEVKRDKRRIEQ
                     LKQTEEFKRQVVCALAHDVKAPLSVILGHAELLAGSFGGQPNPADQTLSLKCIRENID
                     RIVNLVIGFLNVSELVTPRAETSKTAVEINPILRDVIRQQTVALRKKDLNLRLELADD
                     LKPCHGDQNQLERVLWNLIDNAIKYTPPGGAIALASRMFGGKISLSISDSGIGIPKRE
                     LPNLFREFKRLEGSANTEGTGLGLFIVKTIL"
     gene            complement(2101..2277)
                     /locus_tag="VF089_06630"
     CDS             complement(2101..2277)
                     /locus_tag="VF089_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173673.1"
                     /translation="MSPALKVRLFIAIMHLIIIVIMAGSTRPPPDTSDSRRALIDPIT
                     RIRYPESTSHMVGE"
     gene            complement(2324..2527)
                     /locus_tag="VF089_06635"
     CDS             complement(2324..2527)
                     /locus_tag="VF089_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173674.1"
                     /translation="MACLTHHGSACTTSGSSDSSAAGRANGRVLRKVPSPFARGFRML
                     DALIDVALSRAGSNPHQVFIRIK"
     gene            complement(3316..3666)
                     /locus_tag="VF089_06640"
     CDS             complement(3316..3666)
                     /locus_tag="VF089_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6173675.1"
                     /translation="MPLELMDDQELQDHLRDLVSEDGQVDMPELQIIAHNGVVYIEGA
                     LPSEAEHAILLNILTDVAGVQEIVVIWRSNGSLGSVRIAGKKKRLRMSCRGRFRIKNR
                     MAAPTMLCWRMRKA"
     gene            complement(4405..4893)
                     /locus_tag="VF089_06645"
     CDS             complement(4405..4893)
                     /locus_tag="VF089_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173676.1"
                     /translation="MIRSKAIDRAQLFARLDDIRSRTKSSIEISRRLIDQSFLRFEAT
                     HRNLYAAQRLHEQLNALPAKKDPAQEPSNHAGTTERRDTLHSVGLEGNSTCDHCEGVL
                     FNNKAYRVRTQDHGLILLDMVVCYACNLEAQNLGLETDELHAPNTESELTSQNNQVLA
                     LA"
     gene            complement(5212..5790)
                     /locus_tag="VF089_06650"
     CDS             complement(5212..5790)
                     /locus_tag="VF089_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173677.1"
                     /translation="MFELEAFTASCRNALREKNSHAAVQEIVAEAVSQPQQILKSLGE
                     PKLSGIQTLYRSEHLTILNVLWGPGMSLYPHDHRMWAVIGIYSGREDNSFYRRSESGL
                     IVQGGKTLDIKDTIPLGAAVIHSVTNPLDQITAAIHLYGGDFFAVPRSEWDPRTFEER
                     PYDIEHAREVFERANKRMASTELSSIETLMKQ"
     gene            6273..>7369
                     /locus_tag="VF089_06655"
     CDS             6273..>7369
                     /locus_tag="VF089_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6173678.1"
                     /translation="MTSSLGNTMGVRLKPATFTPGVYNRRLPIGAEPTDRGVYFRVWA
                     PQAKRVELLIVPDNFSQAEELKPEAHGYFSGFVPGCAAGDLYHFRVDGGEQLLPDPAS
                     RFQPEGPFGPSMIVDASQFEWHDADWNGMGLANQVIYEMHIGTFTMDGTWQAAGRQLP
                     ELRKLGVTVLEIMPINDFCGTFGWGYDGVNFFAPTRLYGAPDDLRAFVDEAHRAGLAV
                     ILDVVYNHAGPTGNFLKDFSRDYFTDRYENEWGEAINFDGPNSAAVREFFLSNARYWI
                     SEFHFDGLRLDATQALFDASENHILAELVETVRQAATPKSTIVIGENEPQHSELMRPA
                     EDQGYGLDALWNDDFHHTAMVALTGHNEAYYA"
BASE COUNT         1747 a         1850 c         1982 g         1790 t
ORIGIN      
        1 ctagaatagt ttttacgata aacagtccga ggccggtgcc ctcggtgttc gcagatcctt
       61 caagacgctt gaactcccga aacagattgg gcaactctcg tttagggatt ccgataccgc
      121 tatcgctgat gctcagcgaa attttgccgc cgaacattcg cgaagctagg gctatggcgc
      181 cccccggcgg cgtgtatttg atggcattgt cgatcagatt ccaaagcacc cgttccaact
      241 gattctgatc gccatgacaa ggcttcagat catccgcgag ttccaatcgg aggtttagat
      301 ctttcttgcg cagcgcgaca gtttgctgac gtatgacgtc cctgagtatc ggattgatct
      361 cgaccgcagt cttactggtt tccgctctgg gtgtcaccag ctcagagacg tttaaaaagc
      421 cgatgactag gttaactatt cgatcgatgt tttcgcggat gcacttgagc gacagagttt
      481 ggtcagccgg gtttggttgt ccgccgaaag agccggcgag caattcagca tggccgagaa
      541 tgacggaaag cggcgcttta acatcgtgcg cgagggcaca caccacctga cgtttgaatt
      601 cttcggtttg ctttagctgt tcgattcgtc tcttatctcg tttcacctca ttggtaagat
      661 ggccgtacag aactgatatg ccgagcatga acgggactcg cagcagcagg tccgagttga
      721 tagctgacaa gtcgatttgc tgtgagctga caaagatcag gaaaatcaga cttaacaatg
      781 tgcagccaac cacaatatgc aacaggttct cgccggttgc ggcgatgaac acgcagaaga
      841 agaagagcgc cagcaagtcc caaggcgcac tctgagttaa tccgatggca gccagaatga
      901 ggatcgagtc aaaagttaga agtttcgcct caatgttgtt tcccgcaaag aactttagcg
      961 gcagccgctg taggaccgga gctgaaataa tgcaaaggaa tatcagtaac aatggtagag
     1021 gagcgtcgag gccccaggcc tgactggata agattaggta ggatgtgccg actgcaacaa
     1081 accactggag cgctataagc gcgttgcggt tttctgccgg cgaagtctct gtgtccttat
     1141 aggtcaaatc attcctgaat aagcctatga tctgactggc aaggtcggct agtgaatccg
     1201 ttcgggttgc gtcagccggt ttctcgcctt gcgagcgcaa cccgagtctc agccaagtcg
     1261 tcttgagcat ttcaaccatc gagctaggga tactgcggta gcgcttgttt tagcggatcc
     1321 agacaaatga tcgatcgtcg atacctgccg agcaaccaat gtgccacaat gcaagatgca
     1381 gcatgttcaa tgacttacgt tcaaggaaac cagctcattt gtgacaaaat ggttgatcct
     1441 cggaaaagac ggccaaaact gagccgccag ctcaaaataa gtcaaattgc ggagagcgct
     1501 tagacaagat cttcgtcccg ctctcactga ccagcagaac ggcaagatgt tcacgcaagt
     1561 cgtccggcac cggcgctgca tatgagttcc gttgaagagc aggagattac gacgttcagt
     1621 aagcagacgg atctttatca atgaacaact cgaatgcgag gaagattagc tagctaaacg
     1681 ctcgcttcgg gacgattacc ggattggaaa atctacgcac aggtctgata ggagattagg
     1741 atagatgcgg tcgatatttt atgtcggcgc cggcagtttt gttggcggga tttgaggtac
     1801 ttgctgagca cgtggatcta caagcttctt gggaatccgt cgtttccata cggcacactt
     1861 gcagtcaact cgctaggatg tcttgctatt gggtttcttg cagggctagc agaaactcgg
     1921 tcgattttca ccgcgaatga tcgtcttttt atttttgtcg gcatactcgg aggattcaca
     1981 accttctcct ctgtggcgct ggaaacattg tggctggcaa gaggggctca gagcttggcc
     2041 gcgctaacta acattgggct gcaactgttt cttggactat cggccgtttg gatcggcaat
     2101 ctactcgccc accatatggg aagttgactc cgggtaacga atcctggtga tcggatcgat
     2161 caaagcccgt cgtgaatcag aagtatccgg tggtggtcgg gttgaccccg ccatgatgac
     2221 tattatgatc agatgcatga ttgcaatgaa caagcgaact ttcaaagctg ggctcactac
     2281 ggcgctcctg attgtgctca cggcatgtgc cagtcacagg cctttacttt attcgaatga
     2341 acacctgatg cgggttggac ccagcgcggc tgagcgcgac atcgatcaat gcatccaaca
     2401 tgcggaagcc gcgagcgaag ggcgagggaa ccttgcggag aacgcggccg ttagcacggc
     2461 cggcagcgct gctatcggag ctgccgctgg tggtgcaggc ggagccgtgg tgggtcaggc
     2521 aggccaaggc gccgccatag gcgcggcaag cagcgcggct gcaagcctga tgtattctct
     2581 tttgcgcgga ctgtttggat ccgatccacc ggcaccctct tacaggggac ttgttgaccg
     2641 gtgccttcgc gaaaaaggtt acgagccggt atggaagtaa tatgacgtaa gttctaggac
     2701 ggacttaggc aagccactcg gcgtccgcaa attaggcaga tggtcagcct cagttaagtg
     2761 aaatcttggc taatagagtc acctgatgtt tcgaaaacga tacgaaattg ccatcaaagt
     2821 acatagaagc agggactttg cacaatgcga catgtcaagg ggaaaaatgt ggataagtcc
     2881 tcaaatacat cttttttaag ggaccaccga cttggaaaat agttgacaaa cgtagccaaa
     2941 tatttacttc gcttaaaaat agcacatgtc taacaggatc cgcgtaaccc ccgatgctta
     3001 aagttaaatt gtatcctaat tacaaggcat tttgctcaaa ccctgctctg tcccgcgtct
     3061 ccggtccaca gaaccaaatt atccacaaag ttatccacaa ttttggtgga aaatcgctcc
     3121 cgtgacgttt tgcttagagc ggcatggcgg aattttgtcg cgattttggg actcgaggtg
     3181 gcggttatgc cgctcatgcg atgcttcagt cgccgctccc gcgaacgggg catcgctaat
     3241 acttcagcat tgggattaca gcccgagata ctcaacgacg tggcggagcc ggcggattat
     3301 caggaggttc gtaggtcacg ccttcctcat ccgccaacac aacatcgtcg gtgccgccat
     3361 acggttcttg attcggaatc gtccccggca tgacatcctg agtctcttct tctttccagc
     3421 gatcctcacg ctcccaagcg agccgtttga cctccagata accactattt cctgcacccc
     3481 ggccacgtcg gtaaggatgt tgagtagaat tgcgtgctcg gcctcgctgg ggagggcacc
     3541 ttcgatatag actaccccgt tatgagcgat gatctggagc tcgggcatgt cgacttgacc
     3601 atcttcgctt accaggtcgc gtaggtgatc ttgcaattcc tggtcatcca tcaactcgag
     3661 gggcatacca tcagaccgct gctgccgggt gacatcagat ctgaaccctg aaaatttgct
     3721 gacggaggat ttctgcagac ggacatctta cggtttgcct caaaagagcc gcgcggctgt
     3781 aaaaaaatcg ggattaggtc gattcagttg cttcggagaa ggaatagcag ccacaggcag
     3841 aggcagccca caagagtatt aagtaactgc tgtccagatg gatatctaca actgattttt
     3901 agagagagcg gagcccccgt gcgggatgga tgatcaagct aagtgcgggt gaatctggcg
     3961 atgaaaagaa actgcttcaa gccgatgagg aagaaagcag ctcctgccta aaatacatag
     4021 ggcgcgggca caacgtaggt tgactgattc aaggtaggct cgtcaaacac cgcttagatt
     4081 ctgcgcaaga taaatggtgc tgctccggaa tggttcaagc aagacttgcg gccggcgacg
     4141 ttgaacctcg atatcaccgc cacacactgt caatctaacc ttcgctattc tggcaattgc
     4201 tgcggacgcg agagcgctct ccccgctttc gggtagaacg ttgtcgcttc agatcaatcc
     4261 gcgttctaga tcttccgtcg atttcaaacc gaaagttgtg ctggagcaac acttctatca
     4321 gtctttgggc gagctatcag tctggggcga ggagcacgat catgccttat acctcaaacg
     4381 gaagacggtc aaagctaaac ccgcctaagc cagcgcgagc acttgattgt tctgcgaggt
     4441 cagttctgat tcagtattgg gagcgtgcaa ctcgtcagtt tcgagaccga ggttttgtgc
     4501 ttcgagattg cacgcgtagc atacaaccat gtccagtaag attaatccat gatcctgggt
     4561 tctcactcga taggctttgt tgttgaaaag aacaccctcg caatgatcgc atgttgaatt
     4621 tccctcgagc cctacagagt gcagcgtatc gcggcgttcc gtagttccgg catgatttga
     4681 tggctcttga gccgggtctt ttttcgctgg caatgcgttc agttgctcgt gcaatcgctg
     4741 ggctgcgtaa agattgcggt gagttgcctc aaagcggaga aaactttggt cgatgagacg
     4801 acgtgaaatt tcgattgagc tttttgtcct tgatctgatg tcatcgaggc gggcgaaaag
     4861 ttgcgcccga tcgattgcct tgctgcggat cacgatgctt tcctccttag ttgatgattc
     4921 gccttttgcg gagcaataaa cggacccaac cgtaagctga tggaacttat gagaattaca
     4981 gcgagagcga agccgaagct ggaagattag atgacataaa tgtccatctt ggacaaaaaa
     5041 tgcagcccgg cgcaagcatg cggattgtta atttggagtt ctaatatcag cccagatgaa
     5101 atccgggaac gcatgagggt gagtagggga aaggcgcaga acgattgtaa tcgggctccg
     5161 aagaaagttg cttgccggta ttttcaacgt gctgagctac gatcacacgg gctactgctt
     5221 catgagtgtt tcgatgctag acaactcggt agaagccatt cgcttattcg ctcgttcaaa
     5281 tacttctctg gcgtgctcaa tgtcataggg ccgctcctca aatgtgcgtg ggtcccattc
     5341 gctgcgcggt acggcgaaga aatcgcctcc ataaaggtgg attgcggctg tgatttgatc
     5401 aagcgggttc gtaactgaat gaataacggc agcgccaaga ggaatagtat ccttgatatc
     5461 cagtgtcttt ccgccttgaa cgatgagacc gctctcgctt ctgcgataga aagagttgtc
     5521 ctcacgcccg gagtaaattc caatcaccgc ccacatccgg tgatcatgcg ggtacaatga
     5581 catacccggt ccccagagta cgttgagaat cgttaaatgt tcggaccggt agagggtttg
     5641 aataccggac aacttgggct cgccgagaga ttttaatatc tgctgcggtt gactcactgc
     5701 ttccgccaca atctcctgca ctgccgcatg cgaatttttt tctcgaagag cgtttcgaca
     5761 acttgccgtg aaggcttcga gttcaaacat gagagatctc cttctcaagg agagtcgatc
     5821 ccgaggtggg gtggctcggt tcccttttaa ctcgccgggg agcaaagtca agaactatgt
     5881 ttgcgctgaa tacaggctct ccgctatacg agccagttcc ctccaacatg cccggcagca
     5941 acggattgcg gtctttctta tgtcaattaa catcgtactg cttagcgttg cttctagtct
     6001 ttcaccatcg ttccaccgct cggccagtgt cggtcgtccc aaatttagaa cacagcggcg
     6061 ctcttctata cctgagtagc gcagccgaac ttccagcaag caatttttct gcagtgattc
     6121 agatgtttct aagtttagat gctactggta cgagcattgc acaatctcgg ggcgcagacg
     6181 gcgatcgtgc gtactagctt tagatcgtcc cgctaattcc gttcgaactt tttccctgtc
     6241 attatcgagt tcgatatgcg agattttcta gaatgacatc ttctttgggc aacaccatgg
     6301 gagttaggtt gaaaccggcg acattcactc cgggagttta caaccggcga cttccaattg
     6361 gcgctgaacc caccgatcgt ggagtttact ttcgcgtgtg ggccccgcaa gcgaaacgag
     6421 tcgagctcct gatcgtcccc gataatttct cacaggcgga ggaactaaag cccgaagcgc
     6481 acgggtactt ttccggtttt gtgcccgggt gcgcagcggg ggatctctac cactttcgcg
     6541 tggacggagg ggagcaattg ttgccggatc cagcgtcacg atttcagcca gagggtccgt
     6601 tcggcccctc gatgatcgta gatgcgtctc aatttgaatg gcacgatgct gactggaacg
     6661 ggatgggtct cgccaatcag gttatctacg agatgcacat cggcactttt acgatggacg
     6721 gaacctggca agctgccggc cggcagttgc cggaattacg gaagctcggg gtgacggtgc
     6781 tcgagatcat gccgatcaat gatttctgcg gcaccttcgg ctggggctac gacggcgtga
     6841 attttttcgc gccgacccgg ctttatggcg ctcccgacga tttacgcgcg tttgtcgatg
     6901 aagcgcatcg cgccggtctc gcagtgatcc tcgacgtggt ttacaaccac gcgggtccca
     6961 cgggtaactt cttgaaagat ttctccaggg attactttac cgaccgttat gaaaacgagt
     7021 ggggtgaagc gataaacttt gacggtccca actcggcagc ggtgcgcgag ttctttttga
     7081 gtaatgcgcg ttactggatt tctgagtttc acttcgacgg tctgcgctta gatgcgaccc
     7141 aggcgctgtt cgatgcatcc gaaaatcaca tcctggccga gctggtcgaa accgtgcgcc
     7201 aggcagcgac gccaaaatca acaatcgtca tcggcgaaaa cgaacctcaa cacagcgagc
     7261 tcatgcgtcc ggctgaggac caaggttacg gcctcgacgc attgtggaac gatgattttc
     7321 atcataccgc gatggtggct ttgaccgggc acaatgaagc atactacgc
//
LOCUS       DASUTG010000292         5687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_639590, whole genome shotgun sequence.
ACCESSION   DASUTG010000292 DASUTG010000000
VERSION     DASUTG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639590"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF089_06660"
     CDS             <1..719
                     /locus_tag="VF089_06660"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6173679.1"
                     /translation="GAGGSTREKTVDLVDRIHHQCGLETMCHLTVVGQSKDDARGVLR
                     RLQQKGLENVLALGGDPPQGMADWQPHPDGFQHAVELVREAISFGCFSVAVAGFPEIH
                     PRAVSRTSDLGYLKDKIDAGAQAIITQLFFDNDQYYRYVEDVRKLGIQVPIIPGVLPI
                     VSAPQIRRFTALCCARIPPQLEQALSKVENDDNAATQLGIEYASRQCEALIQFGVSGL
                     HFYSLNKSHSVSAICTNLGI"
     gene            783..2288
                     /gene="lysS"
                     /locus_tag="VF089_06665"
     CDS             783..2288
                     /gene="lysS"
                     /locus_tag="VF089_06665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX6173680.1"
                     /translation="MPTSEKELVEETGEQIEVRRQKLARLRETGHKIYPNDFRPDHTT
                     AEVVSKFNGCREEDLPSVARDISLAGRIVGIRKFGKASFFHLQDRRGRLQIYARKDRL
                     GDDGYALFQSFDVGDIVGVAGHLFRTKTKELTLEAESVRLLTKCLRPLPEKWHGLADV
                     EARYRQRYVDLMVNPEVRDVFQRRSQIVRLVRKFFEERDFLEVETPMMQAIPGGAAAR
                     PFTTHHNALDLELFLRIAPELFLKRLVVGGIERVFELNRNFRNEGISVRHNPEFTMLE
                     FYQAYATFEDLMRLTEELFVSLANEVAGTLQVPYGEHVIDLKPPWRRMTIADAIAHYG
                     CVDTKEIAELSGLQSFAKSQGMQVNNDTPYGTLLVEVFEEVAESKIIQPTFIAGYPIE
                     VSPLARKNDNNPALVDRFELFIAGRELANAFSELNDPADQRQRFVEQMKARAAGDDTA
                     NPIDEDYLRALEYGMPPAAGEGIGIDRLVMFFTNSASIRDVILFPLLRPQR"
     gene            2312..3559
                     /locus_tag="VF089_06670"
     CDS             2312..3559
                     /locus_tag="VF089_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX6173681.1"
                     /translation="MNYELFIGLRYLRARRQETFISLITVISVLGVMIGVMTLNVVMA
                     VMTGFEETLRDRLLGINAHIALLKSGDQLAEYEELLQRVTKHEGVVAASPAIYGQVML
                     TSGSRVSGVVVRGVDPDRVNAVVDLQRYMKQGSIAGLKQQNPVQVQDRTIMLPGVIMG
                     ERLAAQLGVFVGSPIQVVSPLGSPTAIGVIPKVRRFVVTGLLNTGMSEIDSTLVFMGL
                     DDAQKFFELGDAVSNIEIRVDDVNESREIADRIQLQLGFPYITEDWTRLWPNLFSALQ
                     LEKTVYFLVLLLMVLIGAFNIISTLVMVVMEKRKDIAILMSMGATRASIRKIFLLKGF
                     IIGIVGTTLGLLLGLVVCTLIAQYQFELPKDVFLISTVPVRIYFSNFMIVTVASFLVC
                     LLASIYPARQAAKLDPVEIIRYE"
     gene            3552..4238
                     /locus_tag="VF089_06675"
     CDS             3552..4238
                     /locus_tag="VF089_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173682.1"
                     /translation="MSSLLSARELDKSYSEGGREIVVLRQLNLELEMAERLAIVGESG
                     VGKSTLLHILGTLDRPTRGRIIYSGKELPLDDEAAVSAFRNREIGFVFQFHYLLPDFS
                     ALENVMLPALIQGMPLAQAREQAEDLLESVGLKERMSHRPGKLSGGEQQRVSVARSVI
                     LQPKLILADEPTGSLDFRIGQEVHDLLFQLNEQRGVALIIATHNRDFAEKIGRQLELR
                     DGQLFPVPVI"
     gene            4313..>5687
                     /gene="bamA"
                     /locus_tag="VF089_06680"
     CDS             4313..>5687
                     /gene="bamA"
                     /locus_tag="VF089_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX6173683.1"
                     /translation="MKPQLTIVEILQLVRCCVFVLSLTSASALWAQEKVSLKDVRITG
                     NVRVEEDGIRLHIKSRAGADFDPALVEQDVKAIFRMGFFDDVEAQLSPENVLIYSVKE
                     KPYVREVRIQGASQLGRDKIETALGVTPRTVLDRSKVAEGVEKVRRLYGEQGYGNAQV
                     DYAVSVEPNNQAVLVLDINEGNRLLIQKISFEGNRTFSQSELKGLMATKEEWLFSFIT
                     NRGILDRDILTNDVAILGNHYIDHGYIDHKIDEPVVLRGRDGLELVIRVHEGPQYRVG
                     KVEIGGDLIQGGEEMLKQVKITEGQIFRGSRLRDDISTLTDLYSNKGFAFAQIEPVTK
                     VNASEKKVDVALVIAKGPPVYFNRVLVAGNTKTRDKVVRRELLTSEQELYSSAKVTQS
                     RNALQRTGYFEDVQLTTKKTDQPDTVDLLVDVKEGPTGTFTVGAGFSSGDGFIFNASI
                     AEKNLFGR"
BASE COUNT         1325 a         1410 c         1602 g         1350 t
ORIGIN      
        1 acggggcggg cggaagcacg cgcgaaaaaa ccgtcgatct cgttgaccgg attcaccacc
       61 aatgcggcct cgaaaccatg tgtcatttga ccgtcgttgg ccaatccaag gatgatgctc
      121 ggggagtcct caggcgcctg cagcaaaagg gactagaaaa cgtccttgcg ttgggtgggg
      181 atcctccgca aggaatggcg gattggcagc cacatcccga cggctttcag catgccgtgg
      241 aactggttag agaggcaatt tctttcggct gtttctcggt tgccgtggca ggctttcccg
      301 agattcaccc acgggctgta agcaggacct cggatctggg ttacctgaag gacaaaattg
      361 atgccggggc tcaagcgatt attacccaat tattcttcga taacgatcaa tactatcgct
      421 atgtcgaaga tgtcaggaag ctaggcatcc aagtgccgat cattccgggc gttctgccga
      481 tcgtttccgc tccacagatt cgtcggttca cggctttatg ttgcgcgcga atcccgccgc
      541 agttggagca agcgttgagc aaagttgaga acgacgataa tgcggcaacc cagttgggca
      601 ttgaatatgc gtcacgtcaa tgcgaggcgt tgatccagtt cggtgtctca ggtcttcatt
      661 tctactcgtt gaacaaatcc cattcagtca gcgcgatctg cacgaatctc ggaatttaga
      721 gcttttctgc tggttccgtc gttgaattca acagttgccg atagtaggtt tcacagacac
      781 cgatgccgac gtccgaaaaa gaacttgtag aagagaccgg cgagcaaatt gaagtgcgcc
      841 ggcagaagct tgccaggctc agggaaaccg gtcacaaaat ctatcctaat gactttcgac
      901 ccgatcacac gaccgcggaa gtcgtctcca agttcaacgg atgtcgtgag gaagatcttc
      961 cgagtgtcgc aagggatatc tccttggccg ggcgcatcgt gggcatacgt aagttcggca
     1021 aggcctcgtt ctttcacctc caggatcgca gaggcaggct gcaaatttac gctcgtaaag
     1081 atcgtctcgg cgacgacggc tacgctttgt tccagtcgtt tgacgtcggc gacatagtcg
     1141 gcgtagcggg tcatctcttt cgcaccaaaa caaaggaatt gacgctggaa gctgaaagtg
     1201 tccgattgct gacgaaatgt ctccggccat tgccggagaa atggcatggt ttagccgatg
     1261 tcgaggcccg ataccggcag cgctatgtcg acttaatggt gaatcccgaa gtgcgggatg
     1321 tattccaaag gcgctctcag atcgttcgtt tggtgcggaa gtttttcgaa gagcgagatt
     1381 ttctcgaagt ggaaactcca atgatgcagg caattcccgg gggcgctgcc gcccgtccgt
     1441 ttaccacgca tcataatgcc ctggatcttg aactcttcct gcggattgcg cccgagttgt
     1501 ttctgaagcg cctcgtcgtt ggcggcattg aaagagtttt tgagctcaat cgcaacttcc
     1561 gcaacgaagg aatctcggtg cgccataatc ctgaatttac catgctggaa ttttatcagg
     1621 cctacgcaac cttcgaagat ctcatgcgtt taacggaaga gttatttgtc tcgcttgcca
     1681 atgaggtggc cggcacactt caggttccct acggagagca cgtgatcgat ctgaagccgc
     1741 cctggcgaag aatgacgatt gccgatgcca tcgcccatta tggttgcgtt gacacaaaag
     1801 aaattgccga gctcagcgga ttgcaaagct tcgcaaagag tcagggtatg caggtaaata
     1861 acgatacgcc ttatggcact ttgttagtcg aagttttcga agaggtggcg gagtctaaaa
     1921 taatccagcc aacatttatt gccggctacc ccatcgaggt ttctccgctg gcgcggaaaa
     1981 acgacaacaa tcctgcgctg gtggatcgat ttgagctatt tattgccggg cgcgaactgg
     2041 ccaatgcctt ctccgagtta aacgatccgg cggaccagcg gcagcggttt gtcgaacaga
     2101 tgaaggcgcg cgcggcagga gacgatacgg ccaatccgat cgatgaagat tatctgcgcg
     2161 cgcttgagta tgggatgccg ccggcggcag gcgaaggcat cggcattgac cggctggtga
     2221 tgttttttac caattcagcc tccattcgcg acgttattct gtttcccttg ctacgaccgc
     2281 agcgctagcc tccaaaccag ccgcccggaa tatgaattac gagcttttca tcgggctcag
     2341 gtatctgaga gctcgccgac aggaaacttt catctcgctg atcacggtta tttccgtgct
     2401 tggcgtcatg atcggcgtga tgacgttgaa cgtggtcatg gcggttatga cgggtttcga
     2461 ggaaacactg cgcgatcgct tgctcggcat caacgcgcac atcgctttgc taaaatccgg
     2521 tgatcagctt gctgagtacg aagagctact ccaacgagtg accaagcacg agggtgtcgt
     2581 ggccgcctcg ccggcgatct acggacaagt gatgttgaca tcggggtcac gggtttcggg
     2641 cgtggtcgtt cgcggtgtcg atccggaccg tgtcaatgcc gtggtggatt tgcaacgcta
     2701 catgaagcaa ggaagcatcg ccggtctcaa gcagcagaat ccggttcagg ttcaggatcg
     2761 caccatcatg ctcccgggcg tgatcatggg cgagcggctg gcggcgcagc tcggggtctt
     2821 cgtcgggagt ccgattcagg tcgtgtcccc cttgggaagt cctacagcca tcggcgtgat
     2881 cccaaaagtg cgccggttcg tcgtcaccgg gctcttgaat accgggatga gtgaaatcga
     2941 ttcgacgttg gtgtttatgg gacttgacga cgcgcagaag ttttttgagc taggcgatgc
     3001 ggtgagcaat atagagatcc gggttgatga cgttaacgaa tcgcgtgaga ttgccgatcg
     3061 gattcaacta cagttgggat ttccgtatat cacggaagat tggacccggt tatggcctaa
     3121 cctattttcg gcgctccagt tggaaaagac tgtttacttt ttggtgttgc tgttaatggt
     3181 tctaatcggc gcctttaaca tcatttcgac cctggtaatg gtggtgatgg agaaacggaa
     3241 ggatattgcg atcttgatgt cgatgggtgc tacccgagct agcattcgaa agattttttt
     3301 gctcaaagga ttcatcattg gcattgtcgg aacgacgttg ggcctgcttc ttgggcttgt
     3361 ggtctgcaca ttgatcgctc agtaccagtt tgagctgccc aaagatgtgt tcttgatctc
     3421 caccgttccg gtgcggattt atttcagcaa ttttatgatc gtgaccgtgg cgtcgttcct
     3481 tgtatgttta ctcgccagca tctatccggc ccgccaagcc gcgaaactgg atccggtcga
     3541 gatcatccgt tatgagtagc ctcctttcag cgcgcgagct cgataaatcc tacagcgaag
     3601 gaggaaggga aatcgtcgtc ctgcgtcaat tgaacctgga gttggaaatg gccgagcggc
     3661 tggcgattgt cggagaatcc ggtgtcggca agagcacgct cttgcatata ctcgggacgc
     3721 tggatcggcc aaccagggga cgaatcattt attccgggaa ggagttgccg ctggatgacg
     3781 aagccgcggt ttcggctttc cgcaatcgag aaatcggctt tgtctttcag ttccattatc
     3841 tcttgcccga tttttcagct ctcgagaatg tcatgcttcc ggcactgatc caaggaatgc
     3901 ccctggcgca ggcccgggaa caagcggagg atctccttga gtcggtcgga ctcaaagaac
     3961 gaatgagcca tcgcccgggt aagttatccg gcggcgagca gcagcgggta tcggtggcgc
     4021 ggtcggtaat tctacagccc aagttgattc tcgccgacga gccgaccggg tcgttggact
     4081 ttcgcattgg ccaggaggtg catgacctgc tctttcaact caatgagcag cgcggcgtcg
     4141 cgctgattat tgcgacacat aaccgtgact tcgctgaaaa gatcggccgg cagttggagt
     4201 tgcgcgatgg ccaactattc ccggtacccg tgatttagct ttacaagatc aagctaattt
     4261 gctagaaaat tcaccagcgt tatccctcca aagaggtctt gccccgggca gaatgaaacc
     4321 gcaactcacc attgttgaaa ttctccagtt agtgcgttgt tgcgtttttg tcctttcact
     4381 cacgtcggcc tcagccttgt gggcgcagga aaaggttagc ctcaaagacg tcaggatcac
     4441 cggcaatgtg agagtcgaag aagacggtat tcgacttcac atcaaaagcc gagccggtgc
     4501 cgatttcgat cccgctctcg tcgaacaaga tgttaaagcg atttttagga tgggattttt
     4561 tgatgacgtc gaagcccagc tctcgcctga gaacgtttta atctattcag ttaaagagaa
     4621 accctacgtc cgcgaggtaa gaatacaagg ggcttcgcaa ttagggcgcg acaaaattga
     4681 aaccgctctt ggcgtaacac cccgaacggt tcttgaccgc agcaaagttg ccgagggggt
     4741 cgagaaggtc agaaggctgt atggcgagca gggatacggg aacgcacagg tggattatgc
     4801 cgtatcggtt gaacctaaca atcaagccgt tctggtcctt gatataaacg agggaaatcg
     4861 gctgttgatt caaaagatca gcttcgaagg caatcgcacg tttagccaaa gcgaactcaa
     4921 gggcctgatg gcgaccaagg aagagtggct cttttcgttc ataacaaacc gcggcattct
     4981 ggatagagac atcctgacca atgatgttgc gattctagga aatcattaca tcgatcacgg
     5041 ctacatcgat cataagatcg atgagcccgt ggtgttgcgc ggccgcgatg ggctggaact
     5101 ggtgatcagg gtccatgaag ggccgcaata ccgtgtcggc aaggtcgaga tcggcggcga
     5161 cctgattcag ggcggcgaag agatgctgaa gcaggtgaaa atcaccgaag ggcagatctt
     5221 tagaggtagc cgcttgcgtg atgatatttc gaccttgacc gatctctact ccaataaggg
     5281 attcgctttc gcgcaaattg aaccggtcac aaaagttaac gcaagtgaaa aaaaggtcga
     5341 tgttgctctg gtaatcgcca aaggtccacc agtgtacttc aatcgagttc tagtcgcggg
     5401 gaacaccaaa accagggata aggtggttcg tagggagtta ctgacttcgg aacaagagct
     5461 ttattccagt gcgaaagtca ctcagagccg aaacgcgctt caacggacag ggtattttga
     5521 agacgtccaa ctaacgacca aaaaaactga ccaacccgac accgtggatc ttctcgtcga
     5581 cgttaaggaa gggcccaccg gcacattcac cgtgggggcc ggatttagca gcggtgatgg
     5641 ctttattttc aacgccagta tagccgaaaa gaatctattt ggccgcg
//
LOCUS       DASUTG010000293         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1381248, whole genome shotgun sequence.
ACCESSION   DASUTG010000293 DASUTG010000000
VERSION     DASUTG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381248"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1845)
                     /locus_tag="VF089_06685"
     CDS             complement(67..1845)
                     /locus_tag="VF089_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6173684.1"
                     /translation="MNRQQSTETNLSAQSAAVPGRRRVFPLSMGARFGIESWVMIAVT
                     LITAYLVLPPLYSVIQTSLFTTTFTGELDQFTLRYYEELLRELRVLGPLLNTIYFSIG
                     SALLATLIGGTVAWVVTRTDTPMRGLGYFTAFASFGTPFILYTIGWILLLGKAGPVNH
                     WLQILFNQNTPVINVYSMAGMIFIEALLWSPFVFLMLAASFRSMDPSLEEASAACGAR
                     VWQTLRRISLRLMLPAFFSVLLLIFIRAFESFEIPALVGLPGDIRVLTTSIYQDAQRL
                     PPQYGNAGAFAVLLMVVVAATLYVYFRVTRDGDRFHTVTGKGYRPALIRLGRWRYVSA
                     AGLLFYSFVLLVLPFLIILWASLLPFYIQPSLDAVSKFTLKNYILAIHFPKIIASIKN
                     SILLGLASATVVMVLTTLASWIMVRSKLRGRWLLDLLTTLPLLFPGIVMGLAIMRFYL
                     IVPIPIYGTLWILLLAFVTRYIPYGIRYTHSGLLQLHKELEEASYVSGASWLNCMRRI
                     TLPLIMPSFLGGWVFVFLLSAKELSMSILLVSPQTPVVSVAIYELWENGQVGELAAFS
                     VIWTSILVSVAVSYYLFARRFGVQQE"
     gene            complement(2292..3389)
                     /locus_tag="VF089_06690"
     CDS             complement(2292..3389)
                     /locus_tag="VF089_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173685.1"
                     /translation="MIHPRLSSKLLLSMSLLGITFGAVTMPGARAASVEEIALLNKPD
                     RQKILVEGAKKEGKISWYTSLIVDQVVRPVKEAFEKEYPFIQVEFFRANSERIVQRMF
                     SEYQAKRYDVDLLDGTVTAAMIRRANFLQRFYSPHLAEHSAETKDPQGNWASTNVYFF
                     ALGYNTRMVKPNELPKTYEDLLNPRWKGQMMWSTSRGSGAPMFIGNVLQSMGESAGKA
                     YLEKLKAQNIAKSTASNRQLLDLVIAGEYPIALHIFNHHAHISKTAGAPVDWQPLEPA
                     TATLNTIALALRAPHPHASMLFLDFVLSERGQKVFQQVNYLPSHPKVPAKQADLKPGG
                     GRFNEALYITPEAQFEKGNDWVDYFNKQFMN"
     gene            complement(3423..>4273)
                     /locus_tag="VF089_06695"
     CDS             complement(3423..>4273)
                     /locus_tag="VF089_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173686.1"
                     /translation="KHIPGNPAIVVENMVGAGSLIAANHVYKIARPDGLTIGHFLGGL
                     FLQQVLGKPGIEFDGKKFAYIGAPAQDSQMLAVSKRSGITNADKWLASRTMVKFGGVG
                     AGSATDDLTKIAIATINAPIQLVSGYKGTAEVRLAFNSGEIDGLTSAWESFKSTWRKE
                     VDAGEVLMILQAIPKPHPELPKVRRVIDYAKTELDRKVIHIGMHNYNPVARPYVLPPD
                     TPKDRVALLRQAFMDTMKDPEFIADATKSKLDLSPLSGEDLEKTVLEIYGAEPDVVAK
                     LKEILK"
BASE COUNT         1065 a         1173 c         1160 g          875 t
ORIGIN      
        1 gctggttcga ctacatgact tgcggagctt taatcctcag agaaggcctc tgggcttagt
       61 tccgcctcac tcctgctgaa ccccgaaccg gcgagcaaac agataatagc tgaccgcaac
      121 gctcaccaag atcgacgtcc agatcacgct gaatgcggcc agctcaccta cctggccatt
      181 ttcccagagc tcgtaaatgg caaccgaaac taccggcgtc tgcgggctca ccaaaagaat
      241 cgacatcgaa agttctttag ctgaaagcaa gaacacgaat acccagccgc cgagaaacga
      301 gggcatgatc agcggcaatg tgatccggcg catacagttc aaccacgagg cgccggacac
      361 atacgaggct tcttccagtt ccttgtgcaa ctgcagcaaa cccgaatgcg tatagcggat
      421 gccgtaaggg atgtagcggg taacgaatgc gagcaacaag atccatagag tgccgtaaat
      481 aggaatcggc acaatcaaat aaaaccgcat gatcgccaat cccatgacga tcccgggaaa
      541 aagcagaggc agtgtggtca acaaatccaa cagccaccgg ccgcgcagct tgctgcgaac
      601 cattatccac gaagccaatg tggtcagcac catcaccaca gtcgcgctgg ccaagcctag
      661 cagaatgctg ttcttgatcg aggcgatgat tttcgggaag tgaatcgcca gaatataatt
      721 cttcagggta aatttcgaga ccgcgtcgag tgacggctgg atatagaacg gcagcaggga
      781 agcccacaga ataatcagaa atggcaggac cagcaggaca aacgaataaa atagcagccc
      841 agcggcgctc acatagcgcc agcggccgag ccggattagc gccggtcgat aaccttttcc
      901 tgtcaccgtg tggaatcgat caccgtcccg ggtaactcga aaataaacgt acaaagtcgc
      961 tgccaccacg accatcagca gaacggcgaa agccccggca ttgccgtact gcggcggcag
     1021 tctttgcgca tcttgataga tggaagttgt gagcacacga atatcgccgg ggagcccgac
     1081 cagagctgga atttcaaatg attcgaaggc gcgaataaaa atcagcagga gcaccgaaaa
     1141 gaacgccggc agcatcaagc gcagcgagat tcgccgcaac gtctgccaca ccctcgcgcc
     1201 acaagcagcc gaggcctcct ccagcgacgg gtccatcgaa cgaaaagacg ccgccagcat
     1261 gaggaaaacg aaaggcgacc aaagcaaggc ttcaatgaag atcatccctg ccatggagta
     1321 aacattgatc accggcgtgt tctgattgaa cagaatctgc aaccagtgat tgaccggacc
     1381 ggctttgccg agaagcaaga tccagccgat cgtgtacaga ataaacggcg ttccaaagga
     1441 tgcgaacgcg gtaaagtagc cgaggccgcg cattggcgtg tcggtgcggg taacgaccca
     1501 agcaacggtg ccgccgatca gagtcgccaa taatgcgctg ccgatagaaa agtaaatcgt
     1561 gttaagaagc ggtcccagca cccgcagctc gcgcagcagc tcttcataat aacgcaaagt
     1621 gaactgatcc agttcgccgg taaaggtggt ggtaaacagg ctggtctgaa tgaccgagta
     1681 gagcggcggc agcaccagat aggcggtgat caatgtgaca gcgatcatca cccaagactc
     1741 gatgccgaag cgcgcgccca tgctcaacgg gaaaacccgg cgtcgccccg gcacggctgc
     1801 ggattgcgca gacaggttag tttccgttga ttgttgccga ttcatagccg actcagagca
     1861 tgatcggaag ctagtctccg ccacattcga aagtcaagat taaatctgac gcagataagc
     1921 taagaagaaa gagaggcacg gcttacccgg aggtccgcag taaaagtttt gcaatgagta
     1981 gccatcggga gactcctccc tgcaaacaga cacagagcag tcggcttgaa ttcgaccaag
     2041 cgattgagag tcaatttcag cacggtgatt gaaggcctcg cccacggtca ctgcttctcc
     2101 actcgcaaag tgccaggaaa gccaaagcgg cgtctaaacc tgacaatttg ctgaaaagct
     2161 ctgaatgcag gctgctcaaa aagacttcac aggcaaggcg cgcgaagttc gatgagctga
     2221 ggcgtacgct tcagtacgtt gaagcgaaac gaacaagcac aacgaagcct gtgaggcttt
     2281 tcagcaggct gctaattcat gaactgcttg ttaaagtaat ccacccagtc attgcctttc
     2341 tcgaactgag cttccggcgt gatgtagagc gcctcgttga aacggccgcc gcccggcttt
     2401 aggtccgctt gtttcgctgg aactttgggg tgggacggca ggtaattgac ctgttgaaaa
     2461 accttttgtc ctcgctcaga caggacaaaa tccagaaaga gcattgacgc gtgcgggtgc
     2521 ggcgctcgta aggcgagggc aatggtatta agagtcgcgg tggcgggctc caagggctgc
     2581 cagtccaccg gggctccggc ggttttgctg atgtgagcat gatgattgaa aatatgcagc
     2641 gcgatcggat attcgccggc gatcaccagg tccaacaact gccgattgct ggccgtactc
     2701 ttggctatgt tctgtgcttt tagtttttcc aggtacgcct tgccggcgct ttctcccatc
     2761 gattgcagaa cgttgccgat aaacatcggc gcgcccgaac cgcggctggt tgaccacatc
     2821 atctggcctt tccaacgcgg attgagaaga tcctcataag ttttcggcag ctcattgggc
     2881 ttgaccatgc gggtgttgta gcccagcgca aaaaagtaaa cgttagtcga cgcccaattg
     2941 ccctgcgggt cttttgtttc cgccgaatgc tcggccagat gaggtgaata gaaacgctgc
     3001 aagaagttgg cgcgccgtat catcgctgcc gtcaccgttc catcgagcaa atcgacatca
     3061 tagcgcttgg cctgatactc gctaaacatt ctttgcacga tccgttcgga attagcgcgg
     3121 aagaactcga cctgaatgaa cggatactcc ttctcgaatg cctccttgac cggacgcacc
     3181 acctgatcga cgatcaacga agtgtaccag gaaatttttc cttctttctt cgctccttcg
     3241 acaagaatct tctgccggtc gggcttgttg agtagggcaa tctcttctac agaggccgcg
     3301 cgtgcgcccg gcattgtgac ggccccaaat gtaatcccca gaagcgacat cgacagcagc
     3361 aacttgcttg agagacgcgg atggatcatg tttacacctc ctgcaaacga gagcgactcg
     3421 gcctacttga gaatttcttt tagcttggcc acgacgtccg gttcggctcc atagatttcc
     3481 agcactgtct tctctagatc ttccccgctg agcgggctta agtcgagctt tgacttggtg
     3541 gcgtcggcga tgaattccgg atctttcatg gtatccataa aggcctggcg caaaagggcg
     3601 acgcgatcct tgggagtgtc aggcggtagg acatagggcc gggccacagg attatagttg
     3661 tgcatgccga tgtgaatcac cttacggtcg agctccgtct tggcgtagtc gattacgcgg
     3721 cgcaccttcg gaagttccgg atgcggcttt ggaatggcct gaaggatcat gagcacctcg
     3781 ccggcatcca cctccttgcg ccaggttgat ttgaacgact cccaggcgct agttaggccg
     3841 tcgatctcgc cgctgttgaa agccaagcga acttccgccg tgcctttgta gccggaaaca
     3901 agctgaatcg gcgcgttgat cgtcgctatg gcgattttcg tcaggtcgtc ggtggcagat
     3961 cccgcgccga ccccgccaaa cttcaccatg gttctcgaag ccagccactt gtcggcattt
     4021 gtgatgcccg atctctttga caccgccagc atctgactgt cctgtgccgg cgctccaata
     4081 taggcgaatt ttttgccgtc aaattcgatt cccggcttgc ctaaaacctg ttgcaggaac
     4141 aagccgccga gaaagtgacc gattgtgagg ccgtcgggcc tggcgatttt gtacacatga
     4201 ttggcggcga taagactgcc ggcgccgacc atattttcca cgacaatggc agggttccca
     4261 ggaatatgct tgc
//
LOCUS       DASUTG010000294         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_639769, whole genome shotgun sequence.
ACCESSION   DASUTG010000294 DASUTG010000000
VERSION     DASUTG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639769"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VF089_06700"
     CDS             <1..124
                     /locus_tag="VF089_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6173687.1"
                     /translation="KTYPGAKHAFNNNTNADRYNPEAAKDAWGRTVSFFKKNLT"
     gene            complement(189..518)
                     /locus_tag="VF089_06705"
     CDS             complement(189..518)
                     /locus_tag="VF089_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173688.1"
                     /translation="MLKATSTRRLIVAALFIASLSPSILNAEEAIEIAAVDVGVTAGN
                     LVFLPAKAVSVIMGGVTGALSFVLTGGNADLTRQIWRDTTEGPYFITPELARKAIGDR
                     PELREPK"
     gene            complement(522..962)
                     /locus_tag="VF089_06710"
     CDS             complement(522..962)
                     /locus_tag="VF089_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173689.1"
                     /translation="MKRTEKSAGNKKKPARKHNNHIQTEQEQATKPTAPSPTVRLAPG
                     STESRSEENSFFKEEKRLFLGVGDEVTHLRYEQWGIGVVMECMTSSVPGGTCLARIRF
                     QDGTLRCFNNDLDNDSCCYYFGLRRYWNPTHGVNAVRSNLFMRG"
     gene            1294..2226
                     /locus_tag="VF089_06715"
     CDS             1294..2226
                     /locus_tag="VF089_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173690.1"
                     /translation="MASQHTPCGIELPQVFLDGPTDMEHIRSFAMQAETLGYDSLWLQ
                     ERIIGDFNMLEPVTLLSYVAGITTKLKLGTSVILLPLRNPFQLAKAYSSLDVMSGGRA
                     VLGVGLGGGHLGSHEDVLGYSREGRVTRFNEAVQIIKLLWTEPKASFQGRYWSFKEIS
                     MEPKPLQKPHLPIIFGGHHDNALRRAVKSANGWMGAGSSSSASFIREASRIRDFLDEA
                     KRDRTSFHISKRVYLAVDKDKARGEQRIREWFARRYKNADLGPKVSIWGSSAECAERI
                     QEIVHAGAQHIVFNPMFDEMEHLEICAKEIMPHL"
     gene            2772..3899
                     /locus_tag="VF089_06720"
     CDS             2772..3899
                     /locus_tag="VF089_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173691.1"
                     /translation="MSKNGLRAFDSDMHVFEPADLYTKYMNPKWGERIPRGQPRTKHG
                     MTRYFLGNGKPIRPPSDVVDFHESRMADRFKESLARDYDNISQLAAMDKEGLDIAVLF
                     RTSPLHTNENFEPEYANDLCKAWNDWMTDFCKADPGRLKGSALITLHDVDLAVKEAKR
                     TVKNGAVGLSLCPEPINGRQIHDRYFDPLWHEAQEMNIPICFHPPARPQQPQVSKDRF
                     EGHPNQALLVNPLRNPIEQILAVSAFTAGGVLERFPQLKVAFLEGNCSWLPWLLYRID
                     EYWEMQGAMADIPLRKKPSEYFKEQCFIAVDVDEALVKNVIEYMGDDNIVISTDYPHI
                     DCRWPHALDEFLMTPISEQSKKKILWDNCARLYGGELDRQC"
     gene            3933..4895
                     /locus_tag="VF089_06725"
     CDS             3933..4895
                     /locus_tag="VF089_06725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173692.1"
                     /translation="MARKLSWMVFLLPLLVHGTGFAQTKIKAAYGAPSLSVIMIQFGA
                     QAGTFATHGLSMEAVYIAGRSINALLSGDVQFGFIGGPQVIQARISGADVLVIGGLNR
                     LGQMIVTHPSIKSPSDLIGKKVGIGVFGTVADYGMRLGLKQFNLRPNKDVTFIQVGDV
                     PARLGAVTSGAVHAVILNSHDKHYLERFGLKLLTETEDVDFLGSALVTTDSYVRSHRD
                     VVIRFARGAAETIRFIKTEPKRSSEILQRIYRENDPAITARRYQLLTGVYADYPYVLP
                     SSIQSVLDVLREDGKIKDAPPAQTFMDMSFLQTVEKERQVSGQK"
     gene            5000..>5451
                     /locus_tag="VF089_06730"
     CDS             5000..>5451
                     /locus_tag="VF089_06730"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173693.1"
                     /translation="MRKSCSFLIGVILALLMHDKAATQTQPLKVVFPSFSGAFTPLWM
                     AHDLGLFTKHGVEIQPVFIEGGSRATQAIVARDIDLGILSGGVIEATLAGAELTFIGT
                     HLKTLVFSLYGPPEIRGVADLKGKTIGVTRFATPAQYSAILALRKFGL"
BASE COUNT         1242 a         1393 c         1551 g         1265 t
ORIGIN      
        1 taagacctac ccaggggcga aacatgcgtt caacaataac acgaacgcag atcgttacaa
       61 tccggaagcg gctaaagacg cctggggaag aacagtcagt tttttcaaga agaacctaac
      121 ttgaaccgcg gacgtaagct gataacggca tgtcgcccca cccttgaggt ggtggttaca
      181 tgccgttatt attttggctc cctgagttcg ggccgatcgc caatggcctt gcgggctagt
      241 tcgggggtta tgaaataagg accttcagtg gtgtcgcgcc agatctgcct tgtcaaatcg
      301 gcattacccc cggttagcac gaaggataaa gctccagtga cccctcccat tattaccgag
      361 accgccttag ccggaaggaa caccaggttg ccagctgtca cgccgacatc aacagcggct
      421 atttcgattg cttcttccgc attgaggatg gatggggaga ggctggcgat gaacaatgcg
      481 gcaacgatca gacggcgagt agacgtggct ttcagcatta attaacctcg catgaaaaga
      541 tttgagcgga ctgcgttgac tccgtgggtc gggttccagt aacggcgcag gccaaagtag
      601 taacagcagc tgtcgttatc gagatcatta ttaaagcagc gcagggtgcc gtcttgaaag
      661 cgaatccggg cgagacaagt gccgccaggc actgaagaag tcatacattc cattacgaca
      721 ccgatccccc actgttcata acggagatgg gtgacttcgt cgccgacccc taagaaaagg
      781 cgcttttctt ccttgaagaa ggaattctcc tccgagcgac tttcggtact gccgggagca
      841 agtcttactg tcggagacgg agccgtcggt tttgttgcct gctcttgctc cgtctggatg
      901 tgattattgt gctttcgggc gggcttcttt ttattccctg cactcttctc agtgcgcttc
      961 acaaatattc ctttgcttca caaatttgct cggcagaatt tggttcttgc tcagtttacc
     1021 ccttcatatt caccaaaatg caggtcgaca gtcaagctcg aaaatctctg gcgcatctcg
     1081 tcaggcgaag tcttcgagcg aagctgagtt gacaccggaa ggcgcgatca gtatgaaggc
     1141 gagagcacgc aggtgccctg cagtttttct cggctgaaac ttgcgctgag atgatctcgt
     1201 tagcgtagaa gtattcactg caggatctcg ttacccttgc ctgcccccaa tggcaggttt
     1261 tagtaaacgt tgaaggggga atgacccata cttatggcta gccagcacac gccgtgcggt
     1321 atcgagctgc ctcaggtttt tctcgatggt cccacggaca tggagcacat tcgcagcttc
     1381 gcaatgcaag cggaaacgct tggctatgac agcttatggc tgcaggaacg gatcatcggc
     1441 gacttcaaca tgctcgaacc ggtgacactg ctgagctatg tcgccgggat cacaaccaag
     1501 ttgaagttag gcacgtcggt cattctattg ccgctgcgca acccgtttca gttagccaaa
     1561 gcgtattcaa gcttggacgt gatgagtggt gggcgcgcgg tgctgggagt gggcctgggt
     1621 ggcggccatc tcggatctca cgaagatgtc cttggctaca gccgcgaagg gcgagtgacc
     1681 cgctttaacg aagcagtgca gatcatcaag cttctttgga cggagccgaa ggcgagtttc
     1741 caaggccgtt actggagctt caaggaaatt tccatggaac ccaagccgct gcaaaagccg
     1801 cacttgccga tcatctttgg cggccaccac gataacgcgc tccggcgggc ggtgaagtcc
     1861 gccaatggtt ggatgggcgc aggctcgtct tcttccgcat ctttcattcg cgaggcgtcg
     1921 cgcattcgtg atttcttgga tgaggccaag cgcgaccgca ccagctttca tatttcgaag
     1981 agagtttatc ttgccgtcga caaggacaag gctcgcggcg agcagcgaat tcgagaatgg
     2041 tttgcccggc gctacaagaa cgctgatctg ggaccgaagg tctcaatctg gggcagcagc
     2101 gccgaatgcg cggaaagaat tcaagagatt gtccatgccg gcgcgcagca cattgtcttc
     2161 aatcccatgt ttgatgagat ggagcatttg gaaatctgcg ccaaagagat catgccacat
     2221 ttgtgagtga gattaactca gagaaatgat cgcaacgagg tcggaatttc tcgcgtaaag
     2281 gcataaagac gccaactgaa gaggagtctc actaggacgg acgagtcggt ctctgttctc
     2341 ccgctatttt tgaaatttga caaagttggc ggtgactctt tcggattaaa ggcttgtcag
     2401 gacgcccacg gttatgcgcc acatgtcagt tgataatgcg accccgcgaa cggattcgtg
     2461 cgcctttctt agtccgagtg tgtggttccg gtcgaccgca agctcggacg tgaagccgct
     2521 cgggcgtgtg caacgtcata ttgcatctgc tgcctgagcc agcttccgcg agggtgcaaa
     2581 gcacttcact ctcgcaaagg caggcttcta acaatctttg actttcaaga gatcaagcaa
     2641 atcgattgcg tcctgtacag cacgccgcgc aattcctttt tagttgagtt ttgattcttg
     2701 cttacacaag atgggatgcg atagaagcga tccatctgat actcgactcg catgaaggga
     2761 ggaatttggg catgtcaaaa aatgggcttc gcgccttcga cagcgatatg catgtcttcg
     2821 agccggccga tttgtacacg aagtacatga atcccaaatg gggcgagcgc attccgcgcg
     2881 gccagccgcg cacaaagcac ggcatgacgc gctatttttt agggaacggc aagccgattc
     2941 ggccgccgag cgacgtggtg gattttcatg aaagccgcat ggccgatcgc ttcaaagagt
     3001 cgctggcgcg ggattacgac aatatttctc aactagcggc catggataag gaaggtttgg
     3061 acatcgcagt cctgtttcgc acttcaccgc tccacactaa cgagaatttt gagccggagt
     3121 acgccaacga tctgtgcaag gcatggaacg attggatgac cgatttctgc aaagcggacc
     3181 ccgggcgtct caaaggatca gcgttgatta cgctccacga cgttgatctt gctgtgaaag
     3241 aagctaaacg tacggtaaag aacggcgcag tcggtttgtc gctttgtccg gagccgatca
     3301 acggccggca gattcacgat cgttacttcg atccgctatg gcacgaagcg caggaaatga
     3361 acattccgat ctgttttcat ccgccggcgc gcccgcagca gccgcaggtg tcgaaagatc
     3421 gcttcgaagg ccatcccaat caggcgcttt tggtcaatcc gttgcgcaat ccgatcgagc
     3481 aaattctggc ggtcagcgcc ttcactgccg gtggtgtgtt ggaacgattc cctcaactga
     3541 aagtggcttt tctcgaaggc aattgcagct ggttgccgtg gttactttat cggattgatg
     3601 agtattggga gatgcagggc gcgatggcag acattccttt acgcaagaag cccagtgagt
     3661 atttcaagga gcagtgcttt atcgcggtcg atgtcgatga ggcattggtc aagaacgtga
     3721 tcgaatacat gggcgacgac aatatcgtca tttccaccga ctacccgcac attgactgcc
     3781 gctggccgca cgcgctcgat gagtttctga tgacgccgat cagcgagcaa tcaaagaaaa
     3841 aaattctctg ggacaattgc gcgcggctgt acgggggtga actagatcga cagtgctgag
     3901 gatggctcca gtgatgtgga aggattttga tcatggctag aaagctttct tggatggttt
     3961 tcctgctccc attgctggta cacggaacag gtttcgcgca gacaaaaatc aaagcggcct
     4021 acggcgcgcc gagcctgagc gtgatcatga tccagttcgg cgctcaagcg gggacgtttg
     4081 ccacgcacgg gctgtcgatg gaagcggtgt atattgccgg aagatcgatc aacgctttgc
     4141 tgtccggcga tgtacagttt ggtttcatcg gcggaccgca agtgattcag gcgcgcattt
     4201 cgggtgccga tgtgctggtc atcggcggtc tcaaccgctt gggacaaatg atcgtgaccc
     4261 atccttcgat caaatcgccc tccgatctta tcggcaaaaa agtcgggatc ggtgtcttcg
     4321 gcacggtggc ggattacggt atgcggttgg ggttgaagca attcaacctg cggcccaaca
     4381 aggacgtgac ctttattcaa gtgggggatg ttccggcgcg cttgggggcg gtgacctcgg
     4441 gcgcggtgca tgcggtgatt ctcaatagtc atgataaaca ctatttggaa aggttcgggc
     4501 ttaagctgct cacggaaacc gaagacgtgg acttcctggg ttcggcgctg gtgaccaccg
     4561 actcttacgt cagaagccat cgcgatgtgg ttatacgatt cgcccgaggc gcagcggaga
     4621 cgatacgctt catcaaaact gagccaaagc gaagttcgga gatcttgcag aggatctatc
     4681 gcgaaaacga tccggcgatc acggcgcgac gatatcagtt gctcaccggc gtctacgccg
     4741 actatccgta cgtcttgccg agttcgatcc agtccgtgct ggatgtcctg cgcgaagatg
     4801 gcaagatcaa ggatgcgccg ccggcgcaaa cattcatgga tatgagtttc ctgcaaacgg
     4861 tggagaaaga gcggcaggta tcgggtcaga agtgatgcgg acccgataac gattcctgcc
     4921 gatcgatctt cgcttgtctc taggtaatag cccggcgaat gctcttatga caaaatttcc
     4981 ggattgcggg gaactcaaag tgagaaagtc atgttcattt cttatcggcg tcattcttgc
     5041 tctgttgatg cacgataaag cggcaacgca aacgcaaccg ctcaaagtcg tgtttccaag
     5101 ctttagcggc gcatttacgc cgctctggat ggcccatgat ctgggccttt tcaccaagca
     5161 tggtgtcgag atccagcctg tctttatcga agggggttcc cgagcgacac aggctatcgt
     5221 tgcgcgggat atcgaccttg gcatcctctc gggcggcgtg atcgaagcga ccctcgccgg
     5281 cgccgagctg actttcatcg gcacgcattt aaagacattg gtctttagtc tttacgggcc
     5341 gccggagatt cgcggcgtgg ccgacttaaa ggggaagacc atcggcgtga cgcggtttgc
     5401 cactccggcg caatatagcg cgatcctggc gctcagaaag ttcgggcttg a
//
LOCUS       DASUTG010000295         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_194881, whole genome shotgun sequence.
ACCESSION   DASUTG010000295 DASUTG010000000
VERSION     DASUTG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194881"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VF089_06735"
     CDS             complement(<1..844)
                     /locus_tag="VF089_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173694.1"
                     /translation="MSGELRKRLYEHFAGIANAAGAKWSVLSGIDGYPEGIGRDLDVA
                     CAARKDADRLCEIFVSCLRQHEFRWIVYPSPIWGRRIMGISRDYETAELHIVHPIRVG
                     TVTLKPDWSAIEYVEGVFPSDPLARFFKRCLMPALAGSAAWQRKCAELPAPGSVPWWM
                     RTFTRKVKSGPAITNLDRLEFYGLYMTRHPLIALDSFCRWCARRLKSRNYPAAPVYQL
                     NGTIDPEVFLSLSQRLLSEVFTGFACIDDLSPNQVRDMQAAQRLVFLSKARSGVRDVC
                     AIPDN"
     gene            complement(878..2005)
                     /locus_tag="VF089_06740"
     CDS             complement(878..2005)
                     /locus_tag="VF089_06740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6173695.1"
                     /translation="MRVAHIGNYKPDSANGVDKTIAGLMRHLPEQGIKVEVWHPSRRV
                     TRIERRIEGGVLVYDLPVRRPFKGLCLFTAETVRFVRSRAQAMDLLHFHSVFLSENLR
                     IASIGIPYVVTPHGGYDELVLSGRNRAAKAIWLKLWERPYLEKARLIQAVSVPELQGL
                     QKLGLRTPLRYVANGIEDCALQLPAPPPSMMSDLVFLGRLAVEQKGLDLLIKGYAMAA
                     ARNHNLPRLVLAGPDFRDGRAQLAAMAEALSISGKVEFAGPLHGEQKWQALSQARVFV
                     HTSRREGMPFALLEAMALGRPTLVTPGTNLADAIAGAKAGFVTECEAGAIADAVCTLA
                     AQPSDTIDTMGERARALVKTQFSWRSIAATMGDAYRGALAD"
     gene            complement(2076..>2719)
                     /locus_tag="VF089_06745"
     CDS             complement(2076..>2719)
                     /locus_tag="VF089_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173696.1"
                     /translation="FGSFGREIALEHWRYGRWTLAAHAISDGYRNLYLFVLPLWAGLG
                     ASGALGALMTLIMPMSFLQQSLNVVTVPTLSRARSTGHFVEKMLLAAAFFLFTAVGFC
                     GAIVLFRSELLGLLYLNRYDAYVDIVLLVALLPVVRSLSFVFDAALSALERPKDIFKA
                     TLPSAACAVTFGIGAVALAGVAGAVLGQLLSAVVGLLTKYWFLRSCNFGRDCR"
BASE COUNT          597 a          867 c          762 g          493 t
ORIGIN      
        1 tattgtccgg gatagcgcac acgtcacgga cccccgatct agccttactc aagaagacca
       61 ggcgctgggc cgcctgcatg tcgcgcacct ggtttggact aaggtcgtca atacatgcga
      121 agcccgtgaa gacctcgctc agcaggcgtt ggctcaggct caggaagact tccggatcga
      181 tcgtgccgtt tagctggtag actggcgccg ccggatagtt ccggcttttc agccgcctcg
      241 cacaccaccg acagaagctg tccaaagcga tcagtggatg tctcgtcatg taaaggccat
      301 aaaactccag gcgatcgaga ttggtaatgg cgggaccgga tttcaccttt ctggtgaaag
      361 ttcgcatcca ccaaggtacg gagccgggtg caggcaattc agcgcacttc cgttgccagg
      421 cagcactgcc cgccaatgcc ggcatcaagc accgcttgaa gaaccgcgcc agcggatcgc
      481 tgggaaagac gccttcgaca tattcaatcg cactccagtc tggcttgagt gtgacggtgc
      541 cgacccggat aggatgaacg atgtgcaact cggccgtttc gtagtcccgg ctgatcccca
      601 tgatacgcct cccccagatc ggactgggat agacgatcca gcgaaactca tgctgtctca
      661 agcaggatac gaagatctcg caaagccgat ccgcgtcctt ccgtgccgcg caggcgacat
      721 ccagatcacg cccgattccc tcggggtacc cgtcgattcc gctcagtacg ctccatttcg
      781 cgccggcggc attcgcgata ccggcgaaat gctcatagag gcgctttctc agctcgcccg
      841 acatgccctc aatgactggc tcctgctcca gtactcgtca atccgccaga gcgccccggt
      901 aagcgtcgcc catggttgca gcaatgctcc tccaggagaa ctgtgttttc accagcgctc
      961 gtgctctctc gcccatcgta tcgattgtat cgctcggctg agccgcaaga gtgcaaactg
     1021 catccgctat tgcccctgcc tcacactccg taacgaagcc tgccttcgcg ccggcgatgg
     1081 catcggccag gttggtcccc ggcgtaacca gggtcggccg gccaagagcc atcgcctcca
     1141 gcaacgcgaa tggcatccct tcccggcggg aagtatgaac gaatactctc gcctgtgaca
     1201 acgcctgcca cttctgttcg ccgtgaagcg gtccggcaaa ttcgaccttg ccggagatcg
     1261 acagcgcctc cgccatggca gccagctgag cacggccatc ccggaaatcg ggcccagcca
     1321 gaaccaagcg cggcaagttg tggttcctgg ccgccgccat cgcgtagcct ttgatcagca
     1381 gatccagacc cttctgctct accgccagtc gacccagaaa caccaaatcc gacatcatgg
     1441 atggcggcgg tgccggcaac tgcagcgcac aatcctcgat gccgttcgcg acatagcgca
     1501 gaggggtgcg cagaccgagc ttctgcaacc cttgcaactc tgggacgctg accgcttgaa
     1561 tcagtcgtgc cttctcaaga taagggcgtt cccataactt cagccatatg gccttggctg
     1621 ctcggttgcg ccccgacagg acaagctcgt catatccgcc atggggcgta acgacataag
     1681 gaataccgat cgatgcgatt ctcaggttct ccgataggaa aaccgagtgg aaatgcagaa
     1741 gatccatggc ctgcgcgcgc gatctgacga agcgcacggt ctcggcggtg aagaggcaca
     1801 ggcccttgaa cggcctgcgg accggcagat catagaccag cacgccgccc tcgatccgcc
     1861 gttcgatccg cgtgacgcgc cgcgaggggt gccatacctc gaccttgatg ccctgctcag
     1921 ggaggtgtcg catcaggccc gcgatcgtct tgtcgacgcc gttggcgctg tcgggcttgt
     1981 agttgccgat atgtgccacg cgcattgccg gtgtccccga aaaagccgat ggacatgcca
     2041 gttgcccgca tttcgtcacg gtaccatgcg ggatgtcacc tgcagtccct cccgaaattg
     2101 caggatcgca agaaccaata cttggtcagc agaccaacaa cggcacttag cagctggcca
     2161 agcacagcac cggcgacgcc agctagtgcg acagcgccga tcccgaacgt aacggcgcac
     2221 gccgcactgg gtagagtagc cttgaaaatg tccttcggtc gttccagggc cgatagggcg
     2281 gcgtcaaaaa caaacgacag cgatcgtacc accggcagca gggccaccag aaggacgata
     2341 tcaacatacg catcgtaacg gttaagatac agcaggccta gtagctcgct gcggaagagg
     2401 acgatggcac cgcagaatcc aactgcggtg aacaagaaaa atgcggccgc cagcagcatc
     2461 ttttcgacaa aatgaccggt gctacgggcg cgcgacaacg tcggaaccgt gacgacgttg
     2521 agtgattgct ggagaaatga catcggcatt atcaaagtca taagggcgcc cagggcacca
     2581 ctggctccca acccagccca cagcggcagc acgaacagat agagattgcg atatccgtcg
     2641 ctgatggcgt gcgccgctag cgtccaacgg ccgtatcgcc agtgctctag ggcaatctcc
     2701 cgcccgaagc tgccgaaaa
//
LOCUS       DASUTG010000296         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_343052, whole genome shotgun sequence.
ACCESSION   DASUTG010000296 DASUTG010000000
VERSION     DASUTG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343052"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..715)
                     /locus_tag="VF089_06750"
     CDS             complement(35..715)
                     /locus_tag="VF089_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6173697.1"
                     /translation="MLGANRAEKKFRASVEDAAGEIRKAILQGKILPGEQLLEEKLGA
                     SFRASRVVLREALRTLEDQGYVTFLPNNQVVASNPSREDMHDYYTIAGVLEGLAARLA
                     VERAQPDEIARLRELHQLLKEACHKGDALQYFDANSNFHRFIAELSHNERLYQLIEEL
                     RREIQKTRLLSLHMPQRLDYSMREHDQIMDAFLKKNPQLAESMMVQHLTNHLKALRKT
                     LDSDSEPR"
     gene            complement(718..>2016)
                     /locus_tag="VF089_06755"
     CDS             complement(718..>2016)
                     /locus_tag="VF089_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6173698.1"
                     /translation="TGIPAVRIETASSAGAAAIQAGFQAVASGYYRRVLVLGGEKMTH
                     LSTSATTRVLAGVIDKEERQCGATMPALAAMITEKYREKFNLSQSRLETMLCAVAVKN
                     HGNGARNPMAQFQKPISRETYLASKYVATPLRLYDCSPITDGAAAVVLTGEKTDVRLA
                     GVGQGTGPLSLRGREVFTSFPATRMAAARAYQMAETSPPGIEFAEVHDAFTPFEIITT
                     EDLGFFAPGKGGDAVLEGVTAAEGRLPINPSGGLKARGHPVGASGLAQIVEVVKTLRG
                     ESASKRQFNRGLTQSTGGLASNNFVTILERTDASLPRSSGFPQPSRHVVKSRPAPAPM
                     SNEGVIETFTILYVTPDGFLPPLALALVREQNGRLILAQGEESKHLKIGREVYLKRTE
                     DYYLFTLKGRFKMVQDSLKRNLWQRARALVRKPLPAGSKL"
BASE COUNT          382 a          603 c          570 g          461 t
ORIGIN      
        1 cttgtgcttg cgcggaggtg agcggcggcc ggatctatcg cggttcgcta tccgagtcga
       61 gcgtctttcg gagagctttc aaatgattcg tcagatgttg caccatcatg ctctcggcca
      121 gctggggatt tttcttgagg aaggcatcca tgatctgatc atgctcgcgc atcgagtagt
      181 cgagtcgctg cggcatatgc aacgatagca gacgagtttt ctgaatttca cgccgcagtt
      241 cctcgatcag ctggtaaagc cgctcgttat gcgacagctc ggctatgaac cgatggaaat
      301 tgctgttggc atcgaagtac tgtagcgcgt cgcctttatg gcatgcttct ttgagcagct
      361 ggtgcagctc gcgcaggcga gcgatttcgt ccggctgagc tctctcgacg gcaaggcgcg
      421 ccgccaaacc ctccagcacg ccggcgatcg tgtagtaatc gtgcatgtcc tcgcgtgacg
      481 ggttgctggc aactacctgg ttgttgggca aaaacgtcac gtaaccctga tcttccaacg
      541 ttcgtagcgc ttcccgaagc accacccggc tggcgcggaa agaggcccca agcttttctt
      601 ccagaagctg ctccccgggc aagatcttgc cttggaggat cgcctttcga atctcgccgg
      661 cggcatcctc aacagatgct ctaaattttt tttctgctcg attggcgccc agcatggtta
      721 tagcttactg cccgcgggaa gcggctttcg tacgagagct ctggcgcgtt gccaaagatt
      781 tcttttcaaa gaatcctgga ccattttgaa acgtcccttg agggtaaaga gatagtagtc
      841 ctcggttcgc tttaggtaaa cttcacgacc gatcttcagg tgcttgcttt cttctccctg
      901 cgccaagatc aggcggccgt tctgttcccg gaccaaggcc agcgccagcg gcggcaaaaa
      961 tccgtcgggg gtgacataga ggatcgtaaa tgtttcgatc acgccctcgt tgctcattgg
     1021 cgccggcgcc ggtcggctct tcacgacgtg ccgagacggc tgcggaaatc ctgaactgcg
     1081 cggcaacgat gcatcagtgc gctccagaat ggtgacgaag ttattgctgg ccagcccccc
     1141 ggtgctctgt gtcaggcctc ggttaaattg acgttttgaa gcgctttcac cgcgcagcgt
     1201 tttcaccact tcaacaatct gcgcgagtcc gcttgcgccc accggatgtc cccgtgcttt
     1261 caaaccgcct gatgggttta tcggcaaacg tccctcggcg gcggtgacgc cttccaggac
     1321 cgcatcgccg cctttgcctg gtgcaaaaaa tcctaaatct tcggtggtaa taatctcgaa
     1381 tggggtgaag gcatcgtgca cctcagcgaa ttcgattccc ggtggcgaag tctcagccat
     1441 ctgataggca cgcgccgctg ccatccgggt cgctggaaac gaggtaaaaa cctctctgcc
     1501 tcgcaagctg agcggcccgg ttccctggcc gacgcccgcc agccgcacgt cggtcttctc
     1561 gccggtaagc acgacagccg cggcgccatc ggtaatcggc gaacaatcat agaggcgcag
     1621 cggagtcgcg acgtatttgc tcgccagata ggtctcgcgg ctgatcggtt tctgaaactg
     1681 ggccatcggg ttacgcgcgc cattcccatg attctttacc gcgacggcgc acagcatcgt
     1741 ctccagccgc gactgagaca gattgaattt ctcgcgatat ttctcagtga tcatcgcggc
     1801 caatgccggc atggtcgcgc cgcactggcg ttcctctttg tcgatgaccc cggccaagac
     1861 tcttgtcgtc gcgctggtgc tcaggtgcgt catcttttcg ccgccgagaa ccaaaacgcg
     1921 gcgatagtaa ccggaggcca ccgcttggaa gccggcctga attgccgccg ctccggccga
     1981 agacgcagtc tcgatacgca cggccggaat accggt
//
LOCUS       DASUTG010000297         8143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_787634, whole genome shotgun sequence.
ACCESSION   DASUTG010000297 DASUTG010000000
VERSION     DASUTG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787634"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /gene="rplV"
                     /locus_tag="VF089_06760"
     CDS             <1..264
                     /gene="rplV"
                     /locus_tag="VF089_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6173699.1"
                     /translation="GVEEALNILKFTPKRSALVVSKTLRTAVANAESTQSVDVDQLYV
                     KQAKVDEAGMWKRFTPRAQGRATRIRKRLSHVTIVLDEVSERR"
     gene            266..928
                     /gene="rpsC"
                     /locus_tag="VF089_06765"
     CDS             266..928
                     /gene="rpsC"
                     /locus_tag="VF089_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6173700.1"
                     /translation="MGQKTHPKMFRLGVIESWDSKWYSRYDYAKLLHEDFRVKKFLKS
                     RLYHAGISKIEIERAANKAKINIHTARPGIVIGKKGAEIEKLKEEIAKLTARETYINI
                     HEVRRPDLDGQLVAENVALQLERRVAFRRAMKESVSRAMRMGAQGVKIQCAGRLGGSE
                     IARTEWYREGRVPLHTLRADVNYGFAEARTTYGIIGVKAWIFRGEVLTEEEEQQKAML
                     GG"
     gene            965..1390
                     /gene="rplP"
                     /locus_tag="VF089_06770"
     CDS             965..1390
                     /gene="rplP"
                     /locus_tag="VF089_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6173701.1"
                     /translation="MLEPKKVKYRKIQKGRRRGTAYRGSTLAFGDFGLKAIDRGWMSA
                     REIEAARVALTRYLKRGGKVWIRIFPDKPLTKKPAETRMGKGKGSPEMWVAVIQPGRI
                     LFEMEGVEASLAREAFRLAAHKLSIPTVVVERKSTVHGS"
     gene            1380..1583
                     /gene="rpmC"
                     /locus_tag="VF089_06775"
     CDS             1380..1583
                     /gene="rpmC"
                     /locus_tag="VF089_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6173702.1"
                     /translation="MGAKEFRDMSSADLQRKLSELREEIGHLKLKRATSRLENPMKLR
                     QTKRDLARVETILKEKADQGGQA"
     gene            1585..1878
                     /gene="rpsQ"
                     /locus_tag="VF089_06780"
     CDS             1585..1878
                     /gene="rpsQ"
                     /locus_tag="VF089_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6173703.1"
                     /translation="MDTRSLRKERTGTVVSDRMEKTIVVSLERTVMHPKYGKYLKRRS
                     RVKAHDETNQCHVGDRVRIVECRPLSRDKRWRVSEILQQTRTTADEQSTGADV"
     gene            1913..2281
                     /gene="rplN"
                     /locus_tag="VF089_06785"
     CDS             1913..2281
                     /gene="rplN"
                     /locus_tag="VF089_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6173704.1"
                     /translation="MIQTRTVLDVADNSGARKVQCIKVLGGSKRKYASIGDIIVVSVK
                     EAIPNAKVKKGEVMKAVVVRTAKEIGRSDGTYIRFDNNSAVLIDNQKEPIGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            2303..2614
                     /gene="rplX"
                     /locus_tag="VF089_06790"
     CDS             2303..2614
                     /gene="rplX"
                     /locus_tag="VF089_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6173705.1"
                     /translation="MQIRKNDSVMVISGKERGKTGKVLRVNPKEDAVIIERINVVKRH
                     TKPRGPQQAGGIVEKEASISASNIMIMCDKCNAPVRIGHKVLADGNKVRVCRRCAEAL
                     D"
     gene            2626..3165
                     /gene="rplE"
                     /locus_tag="VF089_06795"
     CDS             2626..3165
                     /gene="rplE"
                     /locus_tag="VF089_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6173706.1"
                     /translation="MVRLKQKYDEQVVPAMKSEFGYKNFMQVPKLEKITINVGLGEAT
                     QNIKALDSTVAEITAIAGQKPVITRAKKAIANFKLREGVPIGCMVTLRRHRMYEFLDR
                     LIHIALPRVRDFKGVSDRSFDGRGNYSLGLREQIIFPEINPDKVDKARGMTVTITTTA
                     KTDPEGRALLKHLGMPFGA"
     gene            3188..3373
                     /locus_tag="VF089_06800"
     CDS             3188..3373
                     /locus_tag="VF089_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6173707.1"
                     /translation="MAKKCLRIKAERPNKFKVREYNRCPLCGRSRAFYRRFRMCRLCL
                     RDHARKGHIPGLIKASW"
     gene            3386..3790
                     /gene="rpsH"
                     /locus_tag="VF089_06805"
     CDS             3386..3790
                     /gene="rpsH"
                     /locus_tag="VF089_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6173708.1"
                     /translation="MGMTDPIADMLTRIRNASRARHQRVAVPWSRVKENIVKVLVEEG
                     YVKEYKKVKAAEGAGEELIIQLKFDRDNRPIILGLKRVSRPSLKVYVGAQQLTPIRKG
                     LGINILSTSKGILVDREAQRAKVGGEVLCSVW"
     gene            3807..4355
                     /gene="rplF"
                     /locus_tag="VF089_06810"
     CDS             3807..4355
                     /gene="rplF"
                     /locus_tag="VF089_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6173709.1"
                     /translation="MSRIGKLPIAVPSGVKIAVDGDAVRLEGPKGKLTAAIPSGISVK
                     VDGTVVHVQRGEEARKTRALHGLTRKLIANMAEGVSKGFNRILDINGVGYRAEVKGQE
                     IHMTLGYSHPVVFPLPQGVTASVERQVIITLTGPDRQLVGETAAKIRSLREPEPYKGK
                     GIKYREEVIKRKAGKAVGSAGK"
     gene            4371..4736
                     /gene="rplR"
                     /locus_tag="VF089_06815"
     CDS             4371..4736
                     /gene="rplR"
                     /locus_tag="VF089_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6173710.1"
                     /translation="MATKQKNIARIRRQQRVRKKVLGTDARPRVCVYKSSRHIYAQVI
                     SDTQGTTLASASTLSAGLADEVKNIKGVEAAKRVGLNLAKLCKEKNISRVVFDRNGFL
                     FHGRVKAVADGAREGGLEF"
     gene            4762..5259
                     /gene="rpsE"
                     /locus_tag="VF089_06820"
     CDS             4762..5259
                     /gene="rpsE"
                     /locus_tag="VF089_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX6173711.1"
                     /translation="MERIDPHGLELKEKVVHISRVAKVVKGGRRFSFSALIVVGDNHS
                     VVGCGMGKANEVPEAIRKGLEQAKKDLIRVPVMAGTIPFEVTGRFGAGYVILKPASDG
                     TGVIAGGGVRAVVEAAGIQNVLTKCLGSNNPHNMVKATVEALRDLRAPEEIAERRGKT
                     LAEIR"
     gene            5260..5712
                     /gene="rplO"
                     /locus_tag="VF089_06825"
     CDS             5260..5712
                     /gene="rplO"
                     /locus_tag="VF089_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6173712.1"
                     /translation="MKLDDLKPAPGSTKKQKRVGRGDGSGHGKTACRGHKGQRARAGG
                     GTRPGFEGGQMPLQRRLPKRGFNNPFKVDVAIVNLDQLETFSSDSEVNPETLLERGLV
                     HGKNRRIKILGDGALSKSLTIKAHSFSAKAKEKIEAAGGKAELITSHA"
     gene            5705..7015
                     /gene="secY"
                     /locus_tag="VF089_06830"
     CDS             5705..7015
                     /gene="secY"
                     /locus_tag="VF089_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6173713.1"
                     /translation="MLSGFQNASRIPELRKRLFFTLAMLAVYRLGVHVPTPGIDRQAL
                     AAFFEQYKGTIFGLFNLFSGGALEQFSVFSLGIMPYISASIILQLLTVVFPYLERLSK
                     EGEAGRRKITQYTRYGTIVLAVIQGTMISLGLAGTQGPGGQPIVLNADVSFHIMTVIT
                     LASGTAFIMWLGEQITERGVGNGISLIIFAGIVAGLPSAIITTFQFIREGELNVLVAL
                     FMLILIVAVVGAIIFVERGQRRIPVQYAKRVVGRKMYGGQSSHLPLKVNTANVIPVIF
                     ASSILIFPATMAQFAQYPWVQTAATYLSPGQWLHDLFFALLIIFFAFFYTAVVFNPTD
                     VAENMKKFGGYIPGIRPGQKTADYIERVLSRITLSGAIYLCIVALLPTILVSQFNVPF
                     YFGGTALLIVVGVALDTVAQMESHLITRNYEGFMKRGRLRGRRG"
     gene            7019..7681
                     /locus_tag="VF089_06835"
     CDS             7019..7681
                     /locus_tag="VF089_06835"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6173714.1"
                     /translation="MASVRVVLLGPPGAGKGTQAKLLRDRFEACQVSTGDILRKAVAD
                     KTPLGREAEGYLNRGELVPDEVIVRLVGERLKQTDCAKGFVLDGFPRTLPQAQSLESI
                     LKEMALPLDGVLSIQVPHDVIVERLAGRRNCQKCGALYHVNFDPPRNGETCDRCDGRL
                     QQRDDDREETITTRLRVYESQTAPLANYYRERGNLREINGVGKVEDIQRRIVEVLGRP
                     AS"
     gene            7714..>8143
                     /locus_tag="VF089_06840"
     CDS             7714..>8143
                     /locus_tag="VF089_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6173715.1"
                     /translation="MRRANLIVAEVLQELKAKIAPGVTTLDLDRVAEELTLKRNAIPA
                     FKGYSVGGRVFPRCLCASINEEIVHGIPSNRTLKEGDIVGLDYGVIYEGFYGDSAITV
                     GVGKVSDEAKRLMEVTQKSLYDGINQIHEGKRLGDLGAAVQ"
BASE COUNT         2089 a         1849 c         2408 g         1797 t
ORIGIN      
        1 ggggttgaag aggcgctaaa tattttaaaa tttacaccga agcgttccgc tttggttgtg
       61 tccaagactt tgcgcaccgc cgtcgccaac gccgaaagca cgcagagcgt cgatgtggat
      121 cagctctatg tcaaacaggc caaagtcgac gaggccggaa tgtggaagcg atttacgccg
      181 agagcgcagg gaagagccac gcgaatccgt aagcgcttga gtcatgtcac tattgtgctg
      241 gatgaggtga gtgagaggag atagcatggg tcagaaaaca catccaaaga tgtttcgctt
      301 aggtgtcatc gagtcatggg attcaaagtg gtactcccgc tacgattacg ccaagttgct
      361 gcacgaagat ttccgcgtca agaagtttct aaaaagccga ctctatcatg ctggcatctc
      421 aaaaattgaa attgaacgag ccgctaataa ggcgaaaatc aatattcata ccgcgcggcc
      481 cgggattgta atcggtaaaa aaggcgcgga gatcgaaaag ttaaaagagg aaatcgccaa
      541 actcaccgcc cgggaaacct acatcaacat tcatgaagtc aggcggcctg acctcgacgg
      601 ccagctcgtg gccgaaaatg tcgcgctgca gctggaacgc cgggtagctt ttcggcgcgc
      661 catgaaagaa agtgtttcgc gggcaatgcg catgggagct caaggcgtga agattcaatg
      721 cgcaggtcga ctgggcggtt cggaaattgc ccgaaccgaa tggtaccgtg agggccgggt
      781 tccgctccac acccttcggg cagatgtcaa ttatggtttc gcggaggcgc gcacgaccta
      841 cggcatcatc ggcgttaaag cctggatatt ccgcggcgag gtgctcaccg aagaagaaga
      901 gcagcaaaaa gctatgttgg gcggctaaag gcgatgcggt ttaaaataat ttgagacaga
      961 taagatgctc gaacctaaaa aggtaaaata ccgaaagata caaaaaggtc gccggcgagg
     1021 aaccgcatac cgaggttcca ctctagcgtt tggagatttc ggactgaaag cgatagaccg
     1081 gggttggatg agcgcacgag aaatcgaagc ggctcgggtg gctttaacgc ggtatctaaa
     1141 aagaggtgga aaagtctgga tcaggatctt ccccgacaag cccttgacca agaagccagc
     1201 ggaaacccga atgggaaagg gcaagggatc accggaaatg tgggtggcgg tgattcaacc
     1261 cggaagaatt ctgtttgaaa tggaaggcgt ggaggccagc ctcgcgcgcg aggcatttcg
     1321 gttagcggcg cataaattgt ccatcccgac cgttgtcgta gaaagaaaga gcacggttca
     1381 tgggagctaa agaattccgc gacatgagtt ccgctgatct ccagcgtaag ttgagtgagc
     1441 ttcgtgaaga gatcggccat ttgaagctaa agcgggcaac tagcaggttg gagaatccga
     1501 tgaagttgcg gcagaccaag cgtgacttgg ctcgggtgga gacgatcttg aaggaaaagg
     1561 cggatcaagg cgggcaggca tagtatggat actcgaagcc tccgaaaaga acgcaccggc
     1621 acggttgtca gcgaccggat ggaaaagacc attgtggtgt cacttgaacg gaccgtcatg
     1681 catccgaagt acggaaagta tctaaaacgt agaagcagag taaaagcgca cgatgaaact
     1741 aatcaatgtc atgtaggcga ccgggtgcgc atcgttgaat gccggccact cagtcgtgac
     1801 aaacgttggc gcgtcagtga gatattgcaa caaactcgaa cgacagcgga tgagcagtcc
     1861 accggggccg atgtctagcc tccgttcaag tcaagtaggt tgaggttgat tcatgattca
     1921 gacgagaacg gtgttggatg ttgcggataa ctccggtgcg cggaaagtcc aatgtattaa
     1981 ggttttgggt ggcagcaagc ggaagtatgc gtcgatcgga gacattatcg ttgtatcagt
     2041 caaggaagca attcctaacg ccaaagtgaa aaaaggtgaa gtgatgaaag ccgtcgtggt
     2101 aaggaccgcg aaagagattg gacgctcaga cggaacttat attcgcttcg ataataattc
     2161 ggcggtactc atcgataatc agaaggagcc gattggaacc cgaatttttg gtcctgtggc
     2221 gcgtgagctg cgagccaaga agttcatgaa gattatttca ctcgcgcctg aggtgcttta
     2281 aacagttggg gagaacgtgt tcgtgcagat tcgcaaaaac gacagcgtca tggtgatttc
     2341 cggcaaggag cggggtaaga cgggcaaagt cctccgtgtg aacccgaaag aggacgccgt
     2401 gattattgaa agaatcaacg tggtgaagcg gcacaccaaa ccgcgaggcc cgcagcaagc
     2461 cggcggaatc gtcgaaaagg aagcctctat ctcggcatcg aacatcatga tcatgtgcga
     2521 taaatgcaac gcgccggtgc gcattgggca caaagttctc gccgatggca acaaggttcg
     2581 tgtgtgccga cgctgcgccg aggcgttgga ttgatggaaa ccaggatggt tcgtcttaaa
     2641 cagaaatatg acgagcaggt ggtgccggcg atgaagtcgg aattcggtta caagaatttc
     2701 atgcaggtgc cgaagcttga aaagatcact atcaacgtcg gtcttgggga ggcgacgcaa
     2761 aatatcaaag cgttggactc tacggtcgcg gagattaccg ctatcgcggg acagaaaccg
     2821 gtgattacac gggccaagaa agcgatcgcg aattttaaac tgcgcgaagg agtgccgatc
     2881 ggttgtatgg tgacactgcg acgacatcgg atgtatgaat tcctggatag gttaattcac
     2941 atagctctcc cccgagtaag agacttcaaa ggcgtgtctg accgctcctt tgacgggcgc
     3001 ggcaactatt ccttggggct tcgagaacag atcatttttc ccgaaatcaa tcctgacaaa
     3061 gttgacaagg cccggggaat gaccgtcacc ataactacga cagcaaagac cgatcctgag
     3121 ggaagggcgt tacttaagca cttaggaatg ccgtttggcg cttgatcgat aactaggggg
     3181 atatccattg gccaagaagt gtttacgaat caaagcggaa agaccgaata agtttaaagt
     3241 ccgggagtac aaccgttgcc cgctttgcgg acgatcgcgg gcgttttacc gtcgatttcg
     3301 gatgtgccgg ctttgtctta gggaccatgc tcggaagggc catattcccg gacttattaa
     3361 ggccagctgg taggacggag agcatatggg aatgaccgat ccgatcgccg acatgctgac
     3421 gagaatccga aacgcctcga gagcgcggca ccagagggtt gcggtgccgt ggtcgcgagt
     3481 caaagaaaat atcgttaagg tgctcgttga agagggttat gtaaaggaat acaagaaagt
     3541 caaagctgcc gaaggcgcgg gtgaagagct aatcattcaa ctgaagtttg atcgcgacaa
     3601 tcggccgatc atattgggat tgaagcgagt gagtcgccca agcttgaagg tgtacgtcgg
     3661 cgcacagcag ttaaccccga ttcgcaaggg gctaggtatc aacatactct ctacttccaa
     3721 aggaatttta gtcgaccgtg aagcccaacg cgccaaagtt ggcggcgagg ttttatgttc
     3781 ggtttggtag cttgtacgag gtaaacgtgt cacgcattgg aaaattacct atcgctgtcc
     3841 cttcgggagt taagatcgca gtcgatggtg atgcagttcg gctggaagga cccaaaggaa
     3901 aactgactgc ggcgattccc tccggaatat cggtcaaagt tgatggcacc gttgttcatg
     3961 ttcagcgggg cgaagaggcg cgaaaaaccc gagctctgca cggtcttacc cggaaattga
     4021 tcgccaacat ggccgaaggt gtcagcaagg gatttaaccg gatactcgac atcaatggcg
     4081 tcggttatcg cgcagaggtc aagggccaag agattcacat gacactgggg tattctcatc
     4141 cggtggtttt tcccttgcct caaggcgtca ctgcgtccgt cgaacggcag gttatcatca
     4201 ctctaaccgg cccggatcga cagttggttg gggaaactgc ggcgaaaatc agaagtttgc
     4261 gagagcctga gccttacaag ggcaaaggga tcaagtatcg agaagaggtt atcaagcgta
     4321 aggctggcaa agctgtgggt tcagcgggca aatagcatct gaggtcagag atggcaacca
     4381 agcaaaagaa tatcgcgcgc atacggcgac aacaacgcgt ccgcaaaaag gttctgggaa
     4441 ccgatgctcg gcctcgtgtc tgtgtctaca aaagcagtag gcatatctat gctcaggtga
     4501 tatcggacac acagggtacc actttagcat ccgcttcaac gctttcggcc gggctagctg
     4561 atgaagtcaa gaacatcaag ggtgtggaag ccgctaagcg agtcggtttg aatctggcga
     4621 aactttgtaa agaaaagaac attagtcgtg tcgtcttcga tcgtaatggg tttctcttcc
     4681 acgggcgcgt aaaagcggtg gctgatggag cgcgcgaggg tggtttagaa ttttagcgca
     4741 gcctgcatag gtgaggtgtc tttggaacgc attgatccgc atggactaga actcaaggaa
     4801 aaagttgttc atatcagccg ggtggccaaa gtggttaaag gtggccgccg gttcagcttt
     4861 agcgctctga tcgtcgtcgg cgataatcat tccgtggttg gttgcggaat gggtaaggcc
     4921 aacgaagtgc cggaagcgat tcgcaaggga ctggaacagg caaagaaaga tctgatcagg
     4981 gttccggtga tggcaggaac tatccccttt gaggtgaccg gaagatttgg tgccgggtac
     5041 gttatcctga aaccagcttc cgatgggacc ggcgtcattg ccggtggcgg agttcgcgcg
     5101 gtggttgagg cggctggaat tcagaatgtg ttgactaagt gcctgggttc taacaatccg
     5161 cacaacatgg ttaaagctac agtcgaagct ctgcgcgatt tgagagcccc agaggaaatc
     5221 gccgaacggc gcggaaaaac attggcggag atccgttaga tgaagcttga tgatttaaag
     5281 ccggctcccg gctcgacgaa gaaacaaaaa cgtgtggggc gaggcgacgg ttccggtcac
     5341 ggaaaaacgg cttgtcgcgg ccataaaggt caacgcgcac gcgccggcgg tggaactcgt
     5401 cccgggtttg agggcggcca gatgccatta caaagacgtc tgccaaagcg agggtttaat
     5461 aatcccttca aagtggatgt cgcgatcgtg aacctggatc agttggaaac attttcatcc
     5521 gatagcgaag tcaatccgga aaccctgttg gaaaggggat tagtgcatgg gaagaaccgc
     5581 cggattaaga tcctaggcga tggcgcgctg agcaagagtc tgacgatcaa ggcgcatagc
     5641 ttctcggcga aggccaagga gaaaatcgaa gcagccggag gaaaggcgga actgataacg
     5701 tctcatgctt agcggttttc aaaatgcctc gcgcatcccg gagttgcgca aacgtttgtt
     5761 ttttactctg gcaatgcttg ccgtctaccg ccttggggtc catgtgccga cgccgggaat
     5821 cgaccgccaa gcgcttgcgg cgttcttcga acagtataag ggtacgatct ttggcttatt
     5881 caacctcttt tccggtggtg ctcttgaaca attttcggtt ttttcgttgg gcatcatgcc
     5941 ctacatcagt gcgtcgatca tccttcaatt attgaccgtg gtttttccct atctcgaacg
     6001 attgtcaaaa gagggtgaag cagggcggcg gaaaatcacc caatacaccc gttacgggac
     6061 tattgtcctt gcggtcattc agggcactat gatcagcctc ggtttggccg gcacgcaagg
     6121 gcccggtggt cagccgattg tattgaatgc cgatgtgagt tttcacatca tgacggttat
     6181 tacgctcgcc tcgggcacgg cttttattat gtggttgggg gaacagatca ccgagcgggg
     6241 cgtgggtaat ggcatatcgc tgattatatt tgccgggatt gttgcgggtc ttccatctgc
     6301 tatcatcaca accttccagt ttatccggga aggtgagttg aacgtgttag tggcgctctt
     6361 catgctaatt ttgattgtag ccgttgtcgg cgcaattatc tttgttgaac gcgggcagcg
     6421 gcgtattcct gtccagtatg cgaagcgcgt tgtcggtcga aaaatgtatg gtggccagag
     6481 ctcgcatctg ccgttaaagg tgaatacggc caacgtcatc ccggtcattt ttgcttcttc
     6541 aatcctgata tttccagcaa caatggccca atttgcccag tatccctggg tccaaactgc
     6601 ggcgacttat ttgagccctg ggcaatggct gcatgattta tttttcgcgc tgctgatcat
     6661 tttctttgca ttcttctaca ctgcggtcgt gttcaatccg accgatgtcg cggagaatat
     6721 gaagaaattc ggcggctaca ttccaggaat tcgaccgggc cagaagactg ccgattacat
     6781 cgagagggta ctttctcgca tcactctgag tggagcgata tacctgtgta ttgtagcttt
     6841 gctaccgaca atcctggtga gtcagttcaa tgtcccgttc tactttggtg gaaccgcctt
     6901 actcatcgtt gtcggcgtgg cgcttgacac cgtggcgcaa atggagagcc atttgattac
     6961 ccgcaactat gaaggcttta tgaaacgagg acggctgcga gggagaaggg gctaaggttt
     7021 ggccagtgta cgtgttgttc tgctaggccc gccgggcgca ggaaaaggga ctcaggccaa
     7081 actcttgcga gaccggttcg aggcctgtca agtatccaca ggggatatcc taagaaaagc
     7141 tgtcgccgac aagacgccgc ttgggaggga agccgaggga tatcttaatc gcggcgagtt
     7201 ggtgcccgat gaagtcatag tcaggttggt cggtgaacgg ctaaagcaaa cggactgcgc
     7261 caaaggattt gttctggatg gttttcccag aactctgccg caggcccaaa gcctggagag
     7321 tatcttgaag gagatggcgc tgccgcttga tggcgtgttg tctattcaag taccccacga
     7381 tgtcatagtt gagcgcttgg cgggccggcg aaattgccaa aaatgtggcg ctctctacca
     7441 cgtgaatttt gatccacctc gcaatggcga gacctgcgac cgctgtgatg gtcgtctgca
     7501 gcaacgagat gatgatcggg aagagaccat caccactcgc ttaagagttt acgaatcgca
     7561 gactgcgccc ctggctaatt actaccggga gcgcggtaat ttgcgggaaa tcaacggcgt
     7621 tgggaaagtg gaagatattc agaggcgaat tgtcgaggta ctcgggaggc cagccagttg
     7681 atttcgctca aatcacctcg agaaatcgag attatgagaa gagcgaatct gattgtggcg
     7741 gaggttttac aggagctgaa ggcaaagatc gctcccggag tgacgacgct agatctagat
     7801 agagtggcag aagaattgac tctgaaaagg aacgccatcc cggccttcaa aggttattcc
     7861 gtcggcggac gggtcttccc tcgttgctta tgtgcatcga taaacgaaga aatagttcat
     7921 ggaattccat ccaatcggac gcttaaggag ggcgacatcg ttgggctcga ctacggtgtc
     7981 atttacgaag gtttttatgg cgactctgcg atcactgtcg gtgtaggaaa agtcagcgac
     8041 gaagccaagc gcctgatgga ggtcacccaa aagtcattgt atgacggcat caatcaaatt
     8101 cacgaaggca agaggctagg cgatctcggt gctgcggttc aaa
//
LOCUS       DASUTG010000298         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1381645, whole genome shotgun sequence.
ACCESSION   DASUTG010000298 DASUTG010000000
VERSION     DASUTG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381645"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="VF089_06845"
     CDS             <1..2131
                     /locus_tag="VF089_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6173716.1"
                     /translation="PDEFYILATASATEESRVLKMGESFGIFDRYGDVLQYGLGEQGI
                     YHEGTRFLSYLELRLGKIRPFLLSSMIKEDNILFTVDLTNPDVYVDGTIALRRGDVHL
                     YRSKFIWQAVCYERLQLINYSLSPVEISFAIHFDADFADIFEVRGVKREKRGIRLEDE
                     IDGNCVTLSYLGLDEIVRQTIIEFSPLPEKLTAAQASFQRVLDPKKPEAFLISYACRS
                     NHSVPARRPVELALKDAEQALTAYKAEECQIRTSNHEFNGWLDRSYADIHMMITDSQN
                     LYPYAGVPWFSTVFGRDGILTSWELLWIFPRIARGVLTYLAQTQATEVISERDAEPGK
                     ILHEMRRGEMAALGEIPFDRYYGSTDSTPLFVMLAGAYIARTNDGEFAHWIWPHVKRA
                     LHWIKEYGDVDGDGFVETLRRSSHGLVQQGWKDSWDSVFHQDGTLTEGPLALCEVQGY
                     VYAAKHAAAGIAALLGHSDEAVRLVSEAERLKEQFNQVFWCDELSIYALALDQQKRPC
                     RVRASNAGHCLFAGIATDEYAQRTAKTLMSEDFFSGWGIRTLAANEMRYNPMSYHNGS
                     VWPHDNAVIAAGFARYGLKEPTAKVITGLFEASLYLEMHRLPELFCGFPRREGEGPTL
                     YPVACAPQAWAAGSVFLLLQACLGLSVHASEGKVHFDSPVLPECINQIYLKNLRFDSS
                     LLDLVVDRTFQGIGVERRQGDASIVIS"
     gene            complement(2045..3151)
                     /locus_tag="VF089_06850"
     CDS             complement(2045..3151)
                     /locus_tag="VF089_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6173717.1"
                     /translation="MKIAQVSPLYESVPPRTYGGTERIVSYLTEELVAQGHEVTLFAS
                     GDSVTAAQLIAARRRALRLDNVLDPLTHHYVMLEEVFERASRFDIIHFHTDYLHFPLA
                     RRHSVPHVTTLHGRLDLPDLMPVYKKFSEMPVVSISNAQRKPLPRANWQGTVYHGLPA
                     DLYDCGEKPGEYLAFVGRISPEKRLDRAIEIAARFGMKLRIAAKTDAADREYMEQVIR
                     PILRNSSAELIGEIGESEKKEFLANAYALLFPIDWPEPFGLVIIEAMACGTPIIAYRC
                     GSVPELIQEGITGYIVDDVDGALQALGKVSSFDRRRCRRVFEERFSASRMAQDYLSVY
                     RRVIKASADHDARIALAALDSDTLKSTINYQIEK"
     gene            3512..3985
                     /locus_tag="VF089_06855"
     CDS             3512..3985
                     /locus_tag="VF089_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173718.1"
                     /translation="MRKRIINHDAQRSSPLNEGWLDLEKLAQVEVSSEQAQHPIDAAL
                     VPGGSSGWRAAEPGQQIIRLMFDEPQNLHLIRLVFDEHEQERLQEFTLRWSSGVGQPH
                     REIVRQQYNFSPPTTTTQAEDYKVNLDSCTELELSIVPDLRGSSAHASIVEFRLA"
     gene            4046..4213
                     /locus_tag="VF089_06860"
     CDS             4046..4213
                     /locus_tag="VF089_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173719.1"
                     /translation="MIEANTKCPKCGEGIMKIVPEQRALAPDLPEDDELKARCDKCGY
                     EDRVEVVATDE"
     gene            4570..>4991
                     /locus_tag="VF089_06865"
     CDS             4570..>4991
                     /locus_tag="VF089_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173720.1"
                     /translation="MNALELPTVLNVDDNEARRYAKSRILKRGGYQVIESETGAEALR
                     VVKEFQPNLVLLDVKLPDTTGFAVCEAIKADPATAHIMVLQISAMYLSPQDRALGLDR
                     GADTYLTEPVEAAELLGATKALLRLYERDQEKRFLLDQL"
BASE COUNT         1154 a         1323 c         1391 g         1123 t
ORIGIN      
        1 gcccgacgag ttctacattt tggcgaccgc atccgccacg gaagagtctc gggtcctaaa
       61 gatgggcgag agctttggga ttttcgaccg atatggtgat gttctgcaat atggccttgg
      121 cgaacaagga atttatcacg agggaacgcg atttctctcg tatcttgaac ttcgcctagg
      181 caagatccgg ccctttctcc tgagctcgat gatcaaagaa gacaatattc ttttcaccgt
      241 cgatctgacc aatcccgatg tttacgtcga cggcacgatc gcattgcgcc gcggcgatgt
      301 gcatctctat cgctcgaaat tcatttggca ggctgtgtgt tatgagcggc tgcaactgat
      361 caattatagc ttgtcgccgg tagagatttc ttttgcgatt cacttcgacg cggattttgc
      421 cgacatcttt gaggtgcgcg gcgtcaagag agagaagcgc gggatccgcc tggaagatga
      481 gatcgatggt aactgcgtga ccttgagcta tcttggtttg gatgagatcg ttcgccaaac
      541 catcatcgag ttttctccac tccccgagaa gctgacggcc gcccaagcgt cctttcagag
      601 ggttctcgat cctaaaaagc cggaggcatt tttaatctcc tatgcgtgtc gatcgaatca
      661 ttcagttccg gctcggcgtc ccgttgagct ggcgttgaaa gacgcggagc aggcgctgac
      721 cgcatacaag gccgaggagt gccaaatccg cacatcgaat catgaattca acggctggct
      781 cgaccgctcc tacgcggata ttcacatgat gattaccgac agccaaaacc tctaccctta
      841 tgcgggagtg ccctggttca gcacggtgtt cggccgggat ggaattctga catcatggga
      901 gctcttatgg attttccccc ggatcgcgcg cggtgtgctg acgtatctgg cgcaaaccca
      961 agcgaccgaa gtcatctccg aacgcgacgc cgaacccgga aagattctgc acgagatgcg
     1021 tcgcggagaa atggccgctt tgggtgaaat cccttttgac cgctattatg gcagcaccga
     1081 cagcacgccc ttgtttgtca tgctcgccgg cgcttacatc gcgcgcacca acgatggtga
     1141 attcgcgcac tggatttggc cgcacgtcaa gcgcgcgctg cactggatta aagaatacgg
     1201 cgacgtcgac ggcgatggct ttgtcgaaac attgcgccgc tcatcgcacg gtttagtcca
     1261 gcaaggctgg aaagattcgt gggattccgt tttccatcaa gacggaactc tcacggaggg
     1321 accactggcg ctgtgcgaag ttcagggtta cgtttacgcc gccaaacatg cggcggcagg
     1381 gatcgcggct ctcctcggcc atagcgatga agccgtgcgg ctcgttagcg aggccgagcg
     1441 actcaaagaa caatttaacc aagtgttctg gtgtgacgag ctttctatct atgcgttggc
     1501 gctcgaccaa caaaagcgtc cttgcagggt gcgagcatcg aacgcgggcc attgtctgtt
     1561 tgccggtatt gccactgacg agtatgccca gcgtacggca aaaacgttga tgagcgagga
     1621 tttcttttcc ggctggggaa tccggacatt ggcggccaac gaaatgcgtt acaatcccat
     1681 gtcctaccat aacggatcgg tttggccgca cgataatgcg gtgatcgccg caggctttgc
     1741 tcgttatggt ttgaaagaac cgacagccaa ggtgatcacc ggactgttcg aagccagcct
     1801 ctatcttgaa atgcatcgtt tgccggagct cttctgtgga tttcccaggc gcgaaggtga
     1861 aggaccgact ctctatccgg ttgcctgtgc gccccaggca tgggcggcgg gctcggtgtt
     1921 cctattgctg caagcgtgtt tgggactctc ggttcatgcg agcgagggca aggttcactt
     1981 cgattcgcct gtgctgcccg agtgcatcaa tcagatatat ttgaaaaacc tgcggttcga
     2041 ttcgtcactt ctcgatctgg tagttgatcg tacttttcag ggtatcggag tcgagcgccg
     2101 ccagggcgat gcgagcatcg tgatcagctg aagctttgat tacgcggcga tagacgctca
     2161 ggtaatcctg cgccatgcga gaggcgctaa aacgttcttc gaaaacccgg cggcagcgcc
     2221 gccggtcaaa acttgaaact ttaccaagtg cctgaagggc gccatcgacg tcatcgacga
     2281 tgtatcccgt gattccctcc tggatcaact ccggcaccga cccacagcga taagcgataa
     2341 tcggagtgcc gcatgccatc gcctcgatga tcaccaagcc gaacggttcc ggccaatcga
     2401 tcggaaacaa taaggcataa gcattcgcga gaaattcttt tttttcgctt tcaccgatct
     2461 cgccaattaa ttcagccgag gagttgcgca gtatcggcct gatgacctgc tccatatatt
     2521 cacgatcggc ggcatcggtc ttcgcggcga tgcgaagttt catgccaaag cgagcggcga
     2581 tttcgattgc gcgatccagc cgtttctcgg gtgagattcg gcccacgaac gcaagatact
     2641 cgccgggctt ttctccgcaa tcgtaaagat cggcaggcaa gccatgataa accgttcctt
     2701 gccagttggc acgaggaaga ggtttgcgtt gcgcattaga gatcgaaacg accggcattt
     2761 cgctgaattt cttgtaaacc ggcataaggt cgggaagatc gagccgtcca tgtagggtgg
     2821 tcacgtgtgg gaccgagtgt cggcgcgcca gcgggaaatg aaggtaatcg gtatgaaaat
     2881 gaatgatatc gaaccgggac gctctctcga acacctcttc gagcatgacg tagtgatgag
     2941 taagcggatc caatacattg tcgagtcgca aggcacgcct gcgcgccgct atgagttgcg
     3001 ccgcagtaac cgaatcgccg cttgcgaaga gagtgacctc atgtccttgg gccaccaact
     3061 cttcagtcag gtaggaaacg attcgctcag tgccgccgta agttcgcggc ggcacacttt
     3121 cgtacagcgg agaaacttgg gcgattttca tttacttacc cctgcagaat tgccaaggag
     3181 caaatcaaat cggacgaagc aggcaactgt cagatgtcat acttgagcag gagcaaggct
     3241 aatgccaggg atcacttctt ggtttgtcca taagaattaa ccaccagaat tgatgctgct
     3301 gaaagatttc agaaatgcgc ccgccccgtt acgcgcgcta taacggtaca gatagctatg
     3361 tagttgcttc aaccttggga cgtgtaaact ttcggcacgt tgcggcgggg gcaggcctct
     3421 ctttttaccg atcgttaaac tgctaagcta gagtagcgat ccggatcgcc tgaagattgt
     3481 ggagagcagc atccgccgaa ggagaaagca catgcgaaaa cgcatcatca atcatgatgc
     3541 ccaaagatcc tcgccgctga atgaaggctg gctcgatttg gaaaagctcg cgcaggttga
     3601 agtatcttct gaacaagcgc agcatccgat tgacgcggcg ttggtgccgg ggggttcctc
     3661 cggttggcgc gcggcagagc cggggcagca gatcattcgc ctgatgttcg acgagccgca
     3721 gaatctgcat ctcattcgtc tggtgttcga tgagcacgag caggaacggt tgcaggagtt
     3781 tacgctccga tggtcgtcgg gtgtgggcca gcctcatcgg gaaatcgttc gacagcaata
     3841 caactttagc cctccgacaa ccaccacaca ggcggaagat tacaaggtta atctcgacag
     3901 ctgcaccgaa ttggagctca gcattgttcc ggatctcaga ggatcgtcag ctcacgcctc
     3961 gattgtggag tttcgtctgg cgtaaccgac gatgccgaat ctttaaacgc tatcgaacgg
     4021 atactgataa ggggagatca cgcagatgat tgaagccaat accaagtgcc caaaatgcgg
     4081 cgaaggtatc atgaaaatag ttcctgagca gagagccctt gcgccggatc tacccgagga
     4141 cgacgaactc aaggcaagat gcgacaaatg cggctatgag gatcgggtcg aggtggtagc
     4201 gaccgacgaa taagcccact gggaatgctt ttcgacatcc gtcatcatcg ttctgtgaat
     4261 cagcgaattt tcttcgcgca tccgtaatct ttctccaccc cttccggacg gtctagcagg
     4321 gtgctgaaaa aagcctcttg caggctgctc aaaaagatcc agaggcgaga cgcgcgaaaa
     4381 atcgacgagc ggaggcggac ttacagtacg tcgcagtgag gcgattgagc ggcaacgaag
     4441 catatcggtc ttttcagtag cctgctagta atccgggact gcgccggcaa atccatacct
     4501 cgtggcaaaa aactggactt aggtccgatt gacgtatctc cctcgactcg tatacgatcc
     4561 gttatcctca tgaatgcctt ggaactcccc acggttctaa acgtcgacga caacgaagcg
     4621 cggcgctatg ccaagagccg catcctcaaa cgtggcggct atcaggtgat cgagtcggag
     4681 accggcgccg aggcattgcg cgttgtgaag gagttccaac caaatcttgt actgctcgat
     4741 gtcaaactgc cagataccac cgggtttgca gtctgcgagg cgatcaaagc agacccggct
     4801 accgcgcaca tcatggttct ccaaatttcg gctatgtatc tcagccctca ggatcgtgct
     4861 ctcggactcg atcgcggcgc cgacacgtat ttgaccgagc cggttgaagc tgcggagctt
     4921 ctaggggcga caaaggcgtt gttgcgtctg tacgaacgcg accaagagaa acgctttcta
     4981 cttgatcaac t
//
LOCUS       DASUTG010000299         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_195385, whole genome shotgun sequence.
ACCESSION   DASUTG010000299 DASUTG010000000
VERSION     DASUTG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195385"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VF089_06870"
     CDS             complement(<1..1156)
                     /locus_tag="VF089_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6173721.1"
                     /translation="MLSLIGTDVTGIALPWLIKEGIDGAVQKELAGVSLIYFPLLILL
                     AAILQGFFRYCWRININGFSRQAEADLREKVFRHLQKLPLGYFQHNKTGDLMSRLTID
                     VQAVRELLGFGSLASIDAIITIIFSLACMIYIDPWLTLWSLLAMPMITVTVRTFGRHI
                     FRWSHSVQEQLSDISAYVQENLAGMRVVQVYAQEDNQIRGFQKISAECMRRNMWLAIL
                     WGIFWPLMRVVSGIAATIVLWLGGRQVLQGTMSLGEFVAFNGYLGMLTWPLMAIGYVV
                     NQYQRGTAALSRIGEVLDAEPSPRNDAAVFTNQQRASDLAVEFRNLTFNYGDNGPALK
                     NITLASPAGSACAIVGETGSGKSTLVNLLLRLYEPPSETIFIKGMDITKMP"
     gene            complement(1326..2777)
                     /locus_tag="VF089_06875"
     CDS             complement(1326..2777)
                     /locus_tag="VF089_06875"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6173722.1"
                     /translation="MDKVVSLSKRRGFIFQSSEIYGGTGSCWDYGPLGVELKNNVKAA
                     WWRDSVQSRTDMVGLDSSILMHPRVWQASGHLDHFTDPMVDCRACKKRFRSDKIEELE
                     WVHYCAATKGNKFIVPPGQACKHCGARRTLCPACGKGELTEPRQFNLMFKTFMGPVEE
                     DAAVAYLRPETAQGIFVNFDNVLQSMRMKLPFGIAQIGKSFRNEITPGNFTFRSREFE
                     QMEIEFFVLPGTDEEWHQRWLDERFAWYPKHGIRKENLRLREHEKEELAHYAKRTADI
                     EYLFPMGWSELEGVANRTDFDLKQHAQFSGKALEYFDEESKQKVIPYVIEPSAGADRA
                     TLAFLVDAYHEEEVRSEKRVVLKFHPELAPIKIAILPLLKKNDLIVETAKKLMLDLRK
                     HWHAVYDDTASIGRLYRRQDEVGTPFCITIDVQTVGDSKTAADEKVTIRDRNTMQQIR
                     VPLANVEVVMSELMTGHWNDVVRSSGVATVSAN"
     gene            complement(3046..4734)
                     /locus_tag="VF089_06880"
     CDS             complement(3046..4734)
                     /locus_tag="VF089_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173723.1"
                     /translation="MAAPSNSGAAIARSLTLPSALPLAVLPVLALSCLAFFSAPNIIG
                     LFHDDGIYAVVAKALSEGSGYRIISLPGDPYQTKYPFLYSYLLSWAWSFNPHILEIIP
                     LLHFGSVFFYGVSLLLAYVLYVQNSDGRKSDGLLYLFLVGANASVFSMTGYILSDIPF
                     MAACLCSLALSNLEGRFLTRTKAIVLLSASAGIAFMLRQAGAALIFAGLIHFLLSGKR
                     KEFYLFVAIVGCLIVPWLVWQALHASGAVENPLFAYYQSYESPAFFLALSDASAACQI
                     IWDNMRYLVYSIDSMILRLHIIPGLRFIVYPLMLWGLLSIVQRQTIFFTAFLVFYGGL
                     ILSWPFNPGRYFLPLVPVLLLSLFHGAQAASAFLQLRTKTTWSGIIVPVVIRIPLAMV
                     ALLMIGWLWGYVHIDRNQHLVQWGGFRSSYGWSGFSETFSWVKQNTQEDDILAAAYDP
                     MYYLYTGRKAVRPWIHRPETYFYPYGNASPDLGSVEEIRNHLEELGVRFLIVNPMEGY
                     SEGVAVAELFDNLVQSYEVRPELVFESTDQFHRIYALPQPSGKILQPSVRSPVS"
BASE COUNT         1281 a         1326 c         1269 g         1093 t
ORIGIN      
        1 gcggcatctt ggtaatatcc atacccttga taaaaatcgt ttcggacggc ggctcgtaga
       61 gccgcagcag caaattgacg agcgtgcttt ttcccgaacc cgtttcaccg acgatggcgc
      121 atgcgctgcc tgctggactg gccagcgtga tatttttcag agccggtcca ttatcgccgt
      181 aattgaaagt aagattacga aactctacgg ccagatcgct tgcgcgttgc tgattggtaa
      241 aaacggctgc gtcgttgcgc ggcgatggtt cggcgtctag aacttcgcca attctactca
      301 aagccgcggt gcctcgctga tactggttga caacgtagcc gatcgccatt aacggccaag
      361 tgagcatgcc cagataaccg ttgaacgcga caaactcgcc gaggctcatg gttccctgaa
      421 gcacctgccg gccgccgagc cacaaaacta tcgtcgcggc aataccggag accacacgca
      481 tgagcggcca gaatattccc cagaggatcg ccagccacat gtttctgcgc atgcactctg
      541 cgctgatctt ctggaatccg cgaatctgat tgtcctcctg cgcatagacc tgaaccacgc
      601 gcatcccggc gagattctcc tggacataag cgctgatgtc gcttaactgt tcttgcaccg
      661 aatgggacca gcggaagata tgccggccga atgtgcgaac cgtcaccgtg atcatcggca
      721 tcgccagcaa cgaccacaac gtgagccacg gatcgatata gatcatgcag gctagactga
      781 aaatgatcgt gatgatggca tcgatgctgg caagcgaacc gaatccgagc aattcgcgca
      841 ccgcctgaac gtcgatagta aggcgggaca tcaaatcacc cgtcttattg tgctgaaagt
      901 agccgagagg gagcttctgc agatgacgaa aaaccttttc gcgtagatcg gcttccgctt
      961 ggcgtgaaaa cccgttgatg ttgatgcgcc agcagtagcg aaaaaagcct tggaggattg
     1021 ccgccagcag gatcagcaag ggaaaataaa tcagtgagac gccggctaat tccttctgca
     1081 ccgccccatc gatgccctcc ttgatcagcc acggaagtgc gatgcccgta acatccgtac
     1141 cgatcagtga gagcactcct agaatgagct ggcgccgata gcgatcgatg agaaacttga
     1201 tgcgaagata gtagcggtaa atcgtcgtta ccttttaatt accttcagaa ttaagacaaa
     1261 ctgcttgtcc ctggagtcgg ccctgcggta gatccggctc agcgatacgc aacgctggag
     1321 ataattcagt tggccgagac cgtcgcaacg ccggagcttc ggacgacgtc gttccagtgg
     1381 ccggtcatta gttccgacat aacgacttca acattagcca gcggcacgcg aatttgttgc
     1441 atcgtgttcc ggtcgcggat ggtgactttt tcatcggcgg ccgtcttgct atcgccgacg
     1501 gtctgcacgt ctattgtgat gcaaaatggc gtacccactt cgtcctgacg tcgatatagc
     1561 cggccgatgg aggcggtatc gtcgtagaca gcgtgccagt gtttgcgcaa atccaacatg
     1621 agcttctttg cggtctcgac gatgagatcg ttcttcttca acaggggcaa tatggcgatt
     1681 tttatcggcg ccagttcagg atgaaatttg agaacaactc gcttctccga tctaacttcc
     1741 tcttcatgat aggcgtcaac caaaaacgcc agtgtcgcgc gatcggcgcc ggcggaaggt
     1801 tcgatcacat agggaatgac cttctgcttg ctttcttcat caaaatactc caacgctttt
     1861 ccgctaaact gcgcatgctg tttgagatcg aagtcggtgc ggttggcaac cccttccaat
     1921 tcgctccaac ccatcggaaa cagatactcg atatcggcag tgcgcttggc gtaatgcgcc
     1981 agctcttcct tctcatgctc gcgcaatcgc aagttctctt tgcggatgcc gtgtttggga
     2041 taccaagcaa agcgttcatc gagccatctt tgatgccatt cctcgtcggt gccgggcagg
     2101 acgaaaaact caatttccat ctgctcgaat tcacggctgc gaaatgtaaa atttcccggc
     2161 gtgatctcgt tgcggaaaga tttgccgatc tgcgcgatac cgaacggaag cttcatgcgc
     2221 atggactgaa gaacgttatc gaaattgacg aaaatacctt gtgcggtttc cggccgcagg
     2281 taagccaccg cggcatcctc ctccaccgga cccataaaag tcttgaacat caagttgaac
     2341 tggcgcggtt cggtgagctc gcctttgccg caagccgggc acagcgtgcg cctggcgccg
     2401 cagtgtttac aggcctggcc aggaggcacg ataaacttat tgcctttggt tgccgcgcaa
     2461 taatgaaccc attccaattc ttcgatcttg tccgaacgaa atcgcttttt gcaggcgcgg
     2521 caatctacca ttggatcggt gaagtggtcg agatggccgc tcgcctgcca aactctcgga
     2581 tgcatcagga tcgacgaatc caagcccacc atgtccgtcc gcgactgcac gctatcgcgc
     2641 caccacgcag ccttgacgtt atttttgagc tccacgccga gcggtccata gtcccagcac
     2701 gaaccggtgc cgccataaat ctcgcttgat tgaaaaataa agccgcgccg cttagagagc
     2761 gacactacct tgtccatctc taccattgag ccgccttggg tgacttgaga ataggttaat
     2821 ctaatggatg cggcatgcag agtcaaaagt gatcgcggct acaccatggc tgcactcgac
     2881 gatttacaag aggagtttgg cttacttctt tccttggcag gcccgttcca atgcgcagaa
     2941 caaaatctcg ttagctgcgc cgatacgacg aagacatggt tttaagcgat aagtcaaaga
     3001 aagcgacagg ctggtgcgac cggacattcg gacgacctgt cactatcaag aaacaggcga
     3061 cctcacgctg ggttgcaaga ttttcccgga cggttgcggc agtgcataga ttctatggaa
     3121 ctgatcggtg ctttcgaaaa ccagctcagg tcggacttca tacgactgca ctaggttgtc
     3181 gaataactcc gcgacagcca ccccttcgct gtatccttcc ataggattta caatgagaaa
     3241 tcttacgcca agctcttcta ggtgatttct aatctcctca accgatccca gatcggggct
     3301 ggcattgccg tagggataaa aatacgtctc gggacgatgg atccacggcc ttacagcctt
     3361 tcttccagtg tagaggtagt acatcggatc gtacgcggca gctagaatgt catcttcctg
     3421 cgtgttctgt ttaacccaag aaaaagtctc actaaagccg ctccaaccat acgagctccg
     3481 aaaccctccc cattgcacga ggtgttgatt acgatcgata tggacataac cccagagcca
     3541 accaatcatc agcaacgcaa ccatagcaag cggtatacgg atgactaccg ggacaatgat
     3601 cccagaccat gttgtcttcg tgcggagttg gagaaatgcg gatgccgctt gagctccatg
     3661 gaatagcgag agcagcagga cgggaactag aggcaaaaag tagcgtcccg gattaaatgg
     3721 ccaagacaaa attaacccgc cgtagaatac caggaatgcg gtgaaaaaaa tcgtctggcg
     3781 ctggacgatc gagagaagcc cccacagcat cagcgggtat actatgaacc gcaacccggg
     3841 aataatgtga aggcgaagta tcatggaatc tatggaatag accagatagc gcatattgtc
     3901 ccagataatc tgacatgcgg cactggcatc gctaagagcc aaaaaaaacg ccggactctc
     3961 gtacgattga taataagcga agagcggatt ttctacggca cccgaagcat gcagagcttg
     4021 ccagacgagc catgggacta taaggcaccc gacgattgca acgaacaggt aaaactcctt
     4081 ccgctttccg gatagaagaa aatggataag cccggcgaaa atcagtgctg cgcccgcttg
     4141 ccgcaacata aaagcaatcc cggcgctagc gctcaaaaga acaattgctt tggtccgagt
     4201 tagaaatcgg ccttctagat tggacagtgc aagactgcaa aggcacgccg ccatgaatgg
     4261 aatatcgctc aagatataac cggtcatcga gaaaacagag gcatttgccc cgactaaaaa
     4321 cagatacaga aggccatccg actttctacc gtccgaattc tgcacataga gaacatatgc
     4381 cagtagaaga gatacgccat aaaagaaaac gctgccaaaa tgaagcagcg gaatgatttc
     4441 caaaatgtgt ggattaaaac tccaggccca agaaagcagg taggaatata gaaacgggta
     4501 ctttgtctga taaggatcgc cgggcaagct aataattcga tatccgctgc cctcgctcag
     4561 cgcctttgct accacggcat aaattccatc atcgtggaac aggccgataa tgttcggggc
     4621 tgagaaaaac gccaagcagg acagagctag cacaggaaga acagccaggg gcaatgcgct
     4681 tggtaaagtc agtgatcgtg ctatggcagc gccgctatta gacggagccg ccaactcctt
     4741 cgtgcctatt gtaatgggtg gcggaggacg atcgaaggta tgattgggca tgcgatttgg
     4801 agtggcgggt acgtacagat cattttaaac gaaccgacct gattttcacc agtacttaat
     4861 gtatcatcgc ggtactcttc aatgtaattt gcgcaagctt gcgccgaaac tgacaagttc
     4921 tccaccacct actgccttct tcgtcagtgc tcggagtcct atagcatcg
//
LOCUS       DASUTG010000300         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_47460, whole genome shotgun sequence.
ACCESSION   DASUTG010000300 DASUTG010000000
VERSION     DASUTG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47460"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1603
                     /locus_tag="VF089_06885"
     CDS             77..1603
                     /locus_tag="VF089_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6173724.1"
                     /translation="MEKFLLMGDGLRRNASKFAVKIAAKDRFREITYGDLNSRVNQLA
                     HALLSMGVGKGDGVALSVGNRIEHLEIAFATAKIGALALPLDVKWKALELGSVLSFFK
                     PKVLILQPDCVPEFESAKTTQDLSFLRPIVIEDLSYGGLLTGQSSDEPAVEVNEDDPF
                     AVLLTSGTTGFPKGCLSTQRTFVFHCINNAIEKGLGVHDTAILSSPIYFNAGRSFTLG
                     IIYFGGTMILHERFDAEDVLKTVEREEATYIGAVPTMCERMLRVLESKKYDTHSLRCL
                     AITGGKVHPSVLEQLRKTITPNIYRTYASTDSGQMAISKPSDMDYKPDAAGRPVWCVD
                     LRIVDDEDRPVKIGEIGEIVCQSPLATEGYYMNPEATHASFREGWFYTGDLGYFDEEG
                     YLFVAGRKKDMVKSGGISIYPLEIESVIYSHPDVLEAAVIGVPDAQWGEAVKAVVVLK
                     PGLSIQGEELIAFCKARLAPYKVPKSVEISNSLPHTDVGKVNKVKLKEIILGEPDRSS
                     "
     gene            1739..2821
                     /locus_tag="VF089_06890"
     CDS             1739..2821
                     /locus_tag="VF089_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173725.1"
                     /translation="MKDLMRACLCVLSGLILLLSTASAAERPFYQGKTITFFINFSAG
                     GPTDIEGRIVARHLPRHIPGNPTIVVQNMSGGGGVTGINFLGEVAKPDALTLGYFTGP
                     YNHQMMKSTTLRVDLMKVPFISAVQGVTVCYIRSDVPPGIKKPTDLAKAERFRAGGLS
                     FDSNKDLRFRLAFDILGLKYDYVTGYNSSNDARLAVQRNEIQYHDENIPGYRGVVEPQ
                     MVKTGMVTPLYYHDVIRPDGTMVKSPDYPELSSFTEVYTQIHGKAPSGIKYEALKAAN
                     MASQNLNRVALLPPGSPPEAIATMRQAFAALAKDEEFIADAKKVMRFQPRFETGEDGE
                     NLRDKVLTAPTEVVDFVRKFVDQAKK"
     gene            2887..>3759
                     /locus_tag="VF089_06895"
     CDS             2887..>3759
                     /locus_tag="VF089_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173726.1"
                     /translation="MFSRVVVVSFISSLIVVTNAAGQEKVKFPLSASSKTLGYSPLWV
                     AHRQGFFDQQGLDVQLVLVSGADKSTMALMGGSVFASSGAIDAIIGATEQGADLVSTG
                     GVINGLTHMLVAGKKFKTYEDLRGANIGSSGLTSGTAFVLRRMMAAKGLQYPKDYNLI
                     NVGPSAQSFLALTANRVDAALIAVPLSSDAAEMGYNVIGRAADIIPDYQLTEISSRRA
                     WAEKNRPLMVRLMKGMVLAMRWLYDNKEPAIEFLAKEMKLKPQHARRGWEYYTENKIW
                     NPNAEANLEGVQAVI"
BASE COUNT          886 a          948 c         1045 g          880 t
ORIGIN      
        1 tccggggcac tgatctaaat tgtgtccgcg actttttcga taatcgaaaa tctaaaatcc
       61 aaaattcaaa tttgaaatgg aaaaattctt attgatgggg gacggcttgc gccgtaatgc
      121 ctccaagttt gctgtcaaga ttgccgccaa ggatcgcttc cgagaaatta cttatggtga
      181 cctcaattcg cgggtaaatc agctcgccca cgcgctgttg tcgatgggtg tgggtaaagg
      241 cgacggtgtc gcattatccg tcggcaatcg catcgagcat ctggagatcg ctttcgccac
      301 cgccaagatc ggcgcgctgg cgcttccgct cgatgttaaa tggaaagctc tggagcttgg
      361 ttcggtgctc tcatttttca agccgaaggt gctgattctg cagccggatt gtgtcccaga
      421 gttcgagtct gccaagacca ctcaggattt gagctttctc aggccgattg tcatcgagga
      481 tttgagttat ggcggtttac tgacgggaca gagtagcgac gaacccgcgg tcgaagttaa
      541 cgaggacgac ccattcgctg ttttgctgac ctccggtacc acaggttttc ctaaaggctg
      601 tctctcgacc caaaggacgt ttgtctttca ctgcatcaac aacgccatcg aaaaaggcct
      661 tggcgttcac gacaccgcga ttctctcctc gccgatttac ttcaacgccg gccggtcgtt
      721 cacattggga atcatttatt tcggcggaac gatgattctg cacgagcgct tcgatgcgga
      781 ggatgttcta aagaccgtgg agcgcgaaga agctacttac attggcgccg tgccgacgat
      841 gtgcgagcgc atgttgcgcg tgctcgagtc caagaagtac gacacccatt ctttgcgttg
      901 tctggcgatt accggcggca aagttcatcc ttctgtcctc gaacagttaa gaaaaaccat
      961 cacgccaaac atctatcgaa cttacgcctc caccgattcc gggcaaatgg ccatctcgaa
     1021 acccagcgat atggactaca agccggatgc cgccgggcgg ccggtctggt gtgtcgatct
     1081 gcgcatcgta gacgacgagg accggccggt gaaaatcggc gagattggtg aaatcgtctg
     1141 tcagagcccg ctggcgacgg agggttacta catgaatccg gaagcgaccc atgcatcttt
     1201 ccgtgagggc tggttctata ccggcgatct cggttacttc gacgaagaag gctatctatt
     1261 cgtcgccggg cgcaagaaag acatggtcaa gagcggcggc atcagcattt atccgctgga
     1321 aatcgaaagc gttatttaca gccatccgga tgttttggaa gcggcagtaa tcggcgtgcc
     1381 cgatgcgcaa tggggcgagg cggtgaaagc agttgtggta ttaaagccgg gattatcgat
     1441 ccagggcgaa gagttgattg cattctgtaa agcgcggctg gccccgtaca aggttccgaa
     1501 gtctgtcgaa atttctaatt ccttgccgca cacggatgtg ggcaaagtga acaaagtaaa
     1561 gttgaaggaa atcattctgg gtgaaccaga tcggtcgagt tgatgcaatg gattgcgttg
     1621 gttgcaattc ttaggcttac atatctacac gcgattgcat agggattctt ctgactccac
     1681 cggcaaatgg tttagcgtca aaaaagatgg atcggcgaga atgaaaggag agccagtcat
     1741 gaaagatctc atgcgcgctt gtttatgtgt cctttcgggt ttgatcctgc ttctgagtac
     1801 agcttcagcg gctgaaaggc ccttttatca ggggaagacg attactttct tcatcaactt
     1861 ttccgccggc gggcccactg acattgaggg acgcatcgtc gcccggcatc ttcccaggca
     1921 tataccggga aatccgacca tcgttgtgca aaacatgtcc ggcggaggcg gggtcaccgg
     1981 gattaatttt ctcggcgagg tggccaagcc ggacgctctc acgttgggct attttaccgg
     2041 accctataat caccagatga tgaagagtac gactctgaga gtcgatctca tgaaagtgcc
     2101 gttcatttcc gcggttcagg gtgtgacggt gtgttatatc cgctccgacg ttccaccagg
     2161 aatcaagaaa ccgacggatc tcgccaaggc cgagcgcttc cgggcaggcg gcctaagttt
     2221 cgatagtaat aaggacctgc ggtttcggct ggctttcgac atccttggcc tgaagtacga
     2281 ttacgtcacc ggttacaata gcagcaacga cgcgcggctg gcggtgcagc gcaacgagat
     2341 ccagtaccac gatgaaaaca ttcccggtta tcgcggcgtg gtcgaaccgc aaatggtgaa
     2401 aaccggtatg gttactccgc tctactatca tgatgtcatc cgccccgatg gaaccatggt
     2461 aaaaagtccg gattatcctg aactgagttc atttaccgag gtctacacgc aaattcatgg
     2521 gaaggctccc agcgggatca agtacgaagc tctcaaagcc gccaatatgg cgagtcagaa
     2581 tctcaatcgc gtagcgctct tgcccccggg ttcgccgccc gaggccatcg cgacgatgcg
     2641 tcaggcgttt gctgcgttgg ccaaagatga agaatttatc gctgacgcca agaaggtgat
     2701 gcgatttcag ccgcgctttg aaaccggcga ggatggtgaa aaccttcggg acaaagtctt
     2761 gaccgctcct accgaggtag ttgattttgt ccgcaaattt gtcgaccagg cgaaaaaata
     2821 gcttttgcat tgtcgcagta aaccggcaat gaagaaaaga ttttgaacca agacgtgggg
     2881 acaaaaatgt ttagtagagt tgtagtcgtt agttttatct catctctcat cgttgtcacc
     2941 aacgccgccg gacaggaaaa agttaagttc ccgctcagcg cttcctccaa aaccctcgga
     3001 tacagcccgc tgtgggtagc gcaccggcaa ggtttcttcg atcagcaggg cttggatgtt
     3061 cagttggtgt tagtttccgg cgccgacaaa tcgacgatgg cgttgatggg cggatcggtg
     3121 ttcgcatcca gcggcgccat cgatgcgatt atcggcgcga ctgaacaggg cgcagactta
     3181 gtatccacgg gaggagtgat caatggcctg acacatatgc tcgtggcggg aaaaaagttt
     3241 aagacctacg aagatctgcg tggcgcaaac atcggctcat ccggtcttac gtccggaacc
     3301 gcctttgttc tgaggcgtat gatggccgcc aagggtttac agtatccgaa ggactacaac
     3361 ctgatcaacg tcggtccctc ggcgcagtcg ttcttggcgc tcacggcaaa ccgggtagac
     3421 gctgcattga tcgccgtgcc tcttagttcc gacgccgcag aaatgggcta taacgtcatc
     3481 ggcagggcgg cagatatcat tccggattat caactcaccg agatttcgag ccgtcgcgcc
     3541 tgggccgaaa aaaaccggcc cttgatggtt cgtctcatga aaggcatggt gctggcgatg
     3601 cgctggcttt atgacaataa agagccggcc atcgagtttc tggccaagga gatgaagctc
     3661 aagccgcaac atgcgcgtcg tggctgggag tattacaccg agaataagat ttggaatcca
     3721 aatgccgagg ccaatctcga aggtgtccag gctgttatt
//
LOCUS       DASUTG010000301         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_491186, whole genome shotgun sequence.
ACCESSION   DASUTG010000301 DASUTG010000000
VERSION     DASUTG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491186"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VF089_06900"
     CDS             complement(<1..1212)
                     /locus_tag="VF089_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6173727.1"
                     /translation="MAFYQYRAADHAGKIVEGIMEAEAEQGVVLRLHEMGCIPLRIGA
                     SAERAAVASQARLPLFPKRKISQQQLLQFTQELGTLLAAGLPLDRSLSILGNLIEGEE
                     FSKVMRTLLEAVRAGKSLATSMGEHSDVFPKIYINMIRAGESGGILEGVLRYLAEYME
                     RSLALKEDLKSALIYPVILASAAGVSLIVLFLYVIPRFAVIFKDVGQAVPWITRVIIG
                     LSTLLTEYGWLIAIALIAAAAAAIFYLRNPETKAELDRVSLRVWLVGDLVRKFETARF
                     SRTLSALLKGGVPLLEALGTVQGVMGNRLMARAINQVQIRVREGKGMARPLAESGFFP
                     PLALNMIAVGEETGKLETMLGEVAGFYDQEVKRTTKRLTSIIEPVLILGMGLVIGVVV
                     ISMLLAIFSIND"
     gene            complement(1265..2965)
                     /gene="gspE"
                     /locus_tag="VF089_06905"
     CDS             complement(1265..2965)
                     /gene="gspE"
                     /locus_tag="VF089_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX6173728.1"
                     /translation="MESYLNLAERLVERRLLSDDELERVLKLQHEQPASLTRLMVELG
                     FLSEDDLLPVLRDHFNIPLVSLRDIPAVPLPVEFSANLGEFLKTARMVPVKVEGRDLI
                     VATTDPFDIARLHALEVASGLRATPVLAKEKEISARIEALFGSTTASDNTQGAVGREI
                     EGAVDEEDVAHLRDMASEVPVIRLVNQMLVRALESRASDVHIEPFENQLKVRYRIDGI
                     LHEVEPPPRQLKAAVVSRLKILAQLNIAERRLPQDGRIKIRLAGKDVDLRIATVPTLY
                     GESVVIRLLERGQIFTQLETIGFPSDILDHFNGMILKPHGMILVTGPTGSGKTTTLYG
                     ALQKINDPGKKIITIEDPVEYQLSGVNQIQVKPQIGLTFANGLRSIVRQDPDVIMVGE
                     IRDAETAEIAVQAALTGHLVFSTLHTNDAAGAISRLLEMGVQDYLLSSSLLGVLAQRL
                     VRRLCKTCRKEVSLSNVDSNEPELSLPDGNLPQQVWEAVGCNTCSGTGYLGRVGIFEL
                     LPVTSEICKIIVQRADAGTIRSLAVQQGMRLLRDDGWDKVRNGVTTVAEVLRVTREEA
                     "
     gene            complement(2990..>3243)
                     /locus_tag="VF089_06910"
     CDS             complement(2990..>3243)
                     /locus_tag="VF089_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX6173729.1"
                     /translation="SLSYQYHRVKDETVQLLAGVLEMDLETDQGRTTVRFVPGDCLHI
                     YPGMKHRMTAVEECDVLEVSTPELDDVIRLEDRYGRAGS"
BASE COUNT          763 a          935 c          804 g          741 t
ORIGIN      
        1 atcattgata ctaaagatcg ccagcagcat ggagatgaca acgaccccaa tcactaaccc
       61 catccccaga atcagtaccg gctcgatgat tgatgttaat ctttttgtcg ttcgcttaac
      121 ttcttgatcg tagaagcccg cgacttcccc gagcattgtt tccagtttgc cagtctcttc
      181 tcccacagcg atcatgttaa gcgccaaggg cggaaagaaa ccgctttccg ccaacggccg
      241 cgccatgccc tttccttccc gaactcgtat ctgcacttgg ttgatcgccc gtgccatcaa
      301 tctattgccc ataacacctt gcacggtgcc cagcgcttcg agaagcggca cgccgccttt
      361 gaggagcgcc gacagagtgc gggagaacct ggccgtctca aacttccgaa ccagatcgcc
      421 gactaaccag acgcgcaaag aaacgcgatc caactccgct tttgtctcgg gattgcgcaa
      481 gtaaaatatt gccgcggcag cagcggcaat caaggcgatc gcaattagcc aaccgtactc
      541 ggtcaacaag gtgctcaggc caatgatgac tcgcgtgatc caaggaaccg cctgccctac
      601 atctttaaag atcactgcaa accggggaat cacgtaaagg aatagcacta tcaatgaaac
      661 tccggccgca ctggcgagaa tcacaggata aatcagcgcc gatttaagat cctctttgag
      721 tgcgagcgaa cgctccatgt actcagccag atagcgcaaa actccttcca agattccgcc
      781 ggattccccc gctcgaatca tgtttatgta gatttttggg aagacatccg aatgctctcc
      841 catggaagtg gctaatgatt tcccggcgcg caccgcctcc aggagcgtgc gcatgacctt
      901 gctaaactct tctccttcaa tcagattacc gagaatagat aagctgcggt caagcggcaa
      961 gccggcagca agcaatgttc ccagttcttg ggtgaattga agcaattgct gttggctgat
     1021 tttgcgcttc ggaaacagcg gtaagcgagc ctgtgaagcc accgccgcac gttcggccga
     1081 agcgccgatg cgcagtggaa tgcagcccat ctcgtgaagg cgcaacacga ctccctgctc
     1141 ggcctcagct tccatgatgc cttcaacgat cttgcccgcg tgatctgcgg cgcgatattg
     1201 atagaacgcc atgtactctc agataaattt tcgaatccgg caaaaacctg acggagagaa
     1261 tccgttacgc ctcttctctt gtcactctga gcacttccgc aactgtggtg acgccgttac
     1321 gcactttgtc ccaaccgtcg tctctcagca ggcgcattcc ctgttgaacg gcaaggctgc
     1381 gaatcgttcc ggcatcggcg cgttggacga taattttgca gatctcagaa gtcactggca
     1441 gtaattcaaa tattccgact cggcctagat aaccggtgcc gctgcaagta ttgcaaccga
     1501 cggcttccca aacctgctgc ggcaaatttc cgtcgggcaa gctaagttcg ggctcattgc
     1561 tatcaacatt cgataatgaa acctctttac ggcacgtctt acatagccga cgcaccaatc
     1621 tctgcgccag cacaccgagc agagaggatg acaatagata atcttgaacg cccatttcca
     1681 gaagtctgga aatggctccg gcagcatcgt tggtgtgcaa tgtcgaaaag actaagtgtc
     1741 ccgttaaggc cgcttgcact gcgatctccg cggtttccgc atcgcgaatc tcaccgacca
     1801 tgatgacgtc gggatcttgt cgcacaattg aacgaagccc attggcgaat gtcagcccga
     1861 tctgcggctt gacctgtatc tgattgactc cactcagttg atattcgaca ggatcttcga
     1921 tggtgatgat ctttttgcct ggatcgttaa tcttctgcaa cgcgccgtat agggttgtcg
     1981 ttttgccgct tcccgtcggt ccggtcacga gtatcatgcc gtgcggctta aggatcatgc
     2041 cgttaaagtg gtctaaaata tcggagggaa atccgatggt ttcgagctgc gtaaaaattt
     2101 gcccgcgctc gagaagccgg atcaccacgc tctcgccata aagtgtcggc accgtagcaa
     2161 tacgcaaatc cacgtctttt cccgctagtc tgatcttgat ccggccgtcc tgcggcaagc
     2221 gccgttccgc aatattgagt tgagcgagaa tcttcagacg cgaaacaact gcagctttaa
     2281 gctgccgcgg cggcggctcc acctcgtgca agatgccgtc tattcggtaa cgaaccttta
     2341 gctggttttc aaatggctcg atatgaacgt ccgatgcacg gctctccagc gcgcgcacga
     2401 gcatttgatt aaccaagcgg atcactggca cttcggatgc catgtcgcgc aaatgggcaa
     2461 catcttcttc gtcaacggcg ccctcgattt cacggcccac ggcgccttga gtgttatctg
     2521 atgcggtagt gctgccgaat agtgcctcga tacgggcgga gatctccttt tcttttgcca
     2581 gcaccggcgt ggctcgcaac cccgaagcga cttccagagc atgcagccgc gctatatcaa
     2641 acggatcggt ggtagcgacg atgagatcgc gtccttctac tttcaccgga accattcgcg
     2701 cagtttttag gaattcaccg aggttagcgg aaaattcaac cggcaacggc acggccggaa
     2761 tatcacgcaa agataccagc gggatattga agtgatcgcg gagcacaggc agcagatcgt
     2821 cctccgaaag aaatccgagc tcaaccatca gacgcgtcaa agaagctggc tgctcatgtt
     2881 gcagcttcag aacccgctcc aactcgtcat cagaaaggag tcgtcgttcg actaaacgtt
     2941 cggcaagatt caaataagat tccataagcg agtcacttcg ccgatgcgat tagctgccgg
     3001 cccggccata gcggtcttcc agacggatta catcgtccag ttcgggcgtg gacacttcca
     3061 ggacatcgca ttcctccacc gcggtcattc gatgcttcat accagggtat atatggaggc
     3121 aatcgccggg gacgaatcgc acggtggttc ttccctggtc ggtctccaga tccatttcga
     3181 gaactccggc cagcagttga acggtttcgt ccttcacacg atgatactga tagctcagcg
     3241 att
//
LOCUS       DASUTG010000302         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1232700, whole genome shotgun sequence.
ACCESSION   DASUTG010000302 DASUTG010000000
VERSION     DASUTG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232700"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF089_06915"
     CDS             <1..197
                     /locus_tag="VF089_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HEX6173730.1"
                     /translation="IDIEPGESWPVFDQLDFFLGAYVLVSLVYAPPLLLTLASLPVVL
                     IGNIASNAVGYWLGFKKTWI"
     gene            complement(216..860)
                     /locus_tag="VF089_06920"
     CDS             complement(216..860)
                     /locus_tag="VF089_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6173731.1"
                     /translation="MPQTILIADDHRLLREGLRALLERDGFQVVAEADNGRSAVRLAK
                     ELQPDIVITDIAMPDLNGLEATRQICAEAPRAKVLALSMHTESRFVLGILEAGASGYL
                     LKDAAFEELSVAIKAILRDQIYLSPAIAGVVVRQSLGHVGSKPRSERAKLSKREREVL
                     QLIAEGKSTKEIAAKLYVSVKTVETHRKQIMDKLDIYSIAELTKYAIREGVTFL"
     gene            complement(877..3141)
                     /locus_tag="VF089_06925"
     CDS             complement(877..3141)
                     /locus_tag="VF089_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6173732.1"
                     /translation="MEKARYREARWIITIQKPSSKESPQFVRAILDSLHDHIAVVDAE
                     GTIGAVNEAWENFAVQNGAPDTSCVSVGANYLEVCRQAIKAGDQGAEHVLNAIESVLN
                     GTKDHAVMDYECSSPLQERWFKMTVLRQKKPGAGAVITHSDITERKQAEHALRRSEGW
                     FRLLTDSLPVLISYVDEAQRYRFNNKGYEHWFGHSREYVEGKHLRQVLGEKAYEAIRP
                     RVEAALSGQLITFEEFIPYRGAGQRYVRATYVPDRENGRVAGFFALIQDLTERKQAEE
                     EIRESRDKLSTIITGADMGTWDWNVPAGTLDVNDRFCTMLGYGPGSFGTEVRRFFDSL
                     HPDDAKEVSSLVAAHFAGENEFYRCDFRLRMADGSYKWIHDAGRLIERDHTGRPLRMV
                     GIHIDINDRKLVDEALKAREEEFRSMFELAAVGNAQSDPETGRFTRVNRRFCDITGYT
                     EEELLNMTYQDITYPEDRARDTAQIQQVLRGERGTWSIEKRYTQKDGRIIWVSVNGTV
                     MRLDGRPHRTVANIVDITERKSYEEALQAHREALRNLAAEISHTQEKERRQIADDLHD
                     HIGQNLMLAKMKLSELHALLPVQYSASMEQVRTLLEQIIKDTRSVIRDLCPQVLYELG
                     LEVAIDWLVEQTQEKYGLRCVAKITPLRRPIREDRRVILFQAARELLVNVAKHARAKQ
                     AKVILKGDEARVTIQVADDGCGFDPSFLTFANSTARGFGLFSIRERLALLGGELQIDS
                     APERGTRATVTLPA"
     gene            3704..>4402
                     /locus_tag="VF089_06930"
     CDS             3704..>4402
                     /locus_tag="VF089_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6173733.1"
                     /translation="MLAAGNGRRLREFVRQQRGDCLPKQYINFVGNRSMLEHTWDRAE
                     SLIAAQNLFIVIAKQHLEFREVYRQLESKPPQSLIIQPANRETAPGVLLPLLHIYKRY
                     PKAIVAVFPSDHFVLEEDLFMRHVDQAFRVVESDASRMILLGLEPHEPDPEYGYIVPG
                     KRVETFPGDSARQVEMFVEKPCTEAAKKIISRGALWNTMVMVFACKTVLSIIQRAVPE
                     LYHSFGPALDALGTP"
BASE COUNT          962 a         1243 c         1130 g         1067 t
ORIGIN      
        1 gcattgatat cgaaccgggg gagagctggc cggttttcga tcaactggat ttttttctag
       61 gcgcctacgt attagtttcg ctcgtctatg ctccgccgct gttgttgact ctcgcgagcc
      121 tcccggttgt acttataggc aacatcgctt caaacgctgt aggctactgg ctgggcttta
      181 agaagacatg gatttagggc gcagaagctg tgacattaca aaaaagtgac tccctcacga
      241 atggcatact tggtcagttc agcgatgctg tagatgtcca acttgtccat gatctgcttg
      301 cggtgggttt ccaccgtttt cacgctgacg taaagtttcg cggctatttc cttggtggat
      361 ttgccctcgg caatgagctg gagaacttct cgttcccgct ttgacagttt ggcgcgctct
      421 gatctcggtt tggacccgac atgtccgaga gactgtctca caacaacgcc ggctatggcg
      481 gggctgagat aaatttgatc tctcaggatt gctttaatcg cgacggaaag ctcctcaaag
      541 gccgcatcct ttagcaggta tcccgacgcc ccggcttcaa ggattcccag cacgaaacgg
      601 ctctcggtat gcatggacaa agcgaggacc tttgcccgcg gcgcttcggc gcatatctgc
      661 cgggtggcct cgagaccgtt cagatcgggc atggcgatat ccgtgataac gatatccggc
      721 tgcagttctt tggctagtct cactgcgcta cggccattat ccgcttctgc gacaacctga
      781 aagccatcgc gctcaagcaa agcgcgcagt ccttctctta gcagcctgtg atcatcagcg
      841 atgagaatgg tttgaggcat gggtcgttta cccgggttaa gcgggaagcg tcaccgtcgc
      901 tctcgtaccc ctttccggag cagaatcgat ctgcagctca ccgcccagga gcgctaggcg
      961 ctcgcggatg ctgaacaacc cgaaccccct ggcggtggaa ttggcaaaag tcaaaaaaga
     1021 cgggtcgaac ccgcaaccat cgtccgcgac ctgaatcgtg acgcgcgcct catccccttt
     1081 cagtataacc ttcgcctgtt tcgctcgggc atgcttggca acgttaacga gcagctcgcg
     1141 agccgcctgg aagaggatca cccggcggtc ttccctgatc ggtcttcgca atggcgttat
     1201 cttcgccacg cagcgcaggc cgtatttctc ctgagtctgc tccaccagcc aatcgatagc
     1261 cacttccaat cccagctcgt agagcacctg cgggcagaga tcgcgaatca cagagcgcgt
     1321 gtctttgatg atctgctcga ggagcgtgcg gacctgttcc atcgaagccg agtactgaac
     1381 gggcagcaaa gcgtggagct cgcttagctt cattttcgca agcatcaaat tttgcccgat
     1441 atgatcatga agatcgtcgg cgatttggcg cctttctttt tcttgggtgt gcgaaatttc
     1501 tgcggcgaga tttctcagcg cctccctgtg cgcttgaagc gcctcctcat aggacttacg
     1561 ctctgtaata tcgacgatat ttgcaacggt ccgatgaggc ctgccatcaa gtcgcatcac
     1621 cgttccattg acggaaaccc aaattatcct gccgtccttt tgggtatagc gcttttctat
     1681 tgaccacgta cccctttctc ccctcaagac ctgctgaatc tgcgcggtgt cgcgggcccg
     1741 atcctcggga taggtgatat cctgataggt catgttgaga agctcctctt ccgtgtaacc
     1801 tgtgatatcg caaaacctcc ggttaacgcg cgtaaaacgg cctgtttcag ggtcggattg
     1861 cgcattgccc acggcggcga gctcgaacat ggagcgaaac tcttcttccc tggccttgag
     1921 agcctcgtcg accagcttgc gatcgttaat atcgatgtgg atgccgacca tgcgtagcgg
     1981 tcttccggtg tgatctcgtt cgatcagccg gcccgcgtcg tgaatccatt tgtaggagcc
     2041 gtccgccatc cgcaaccgga aatcgcaacg atagaattca ttttcgccag cgaagtgggc
     2101 cgccacgaga ctactaacct ctttcgcatc gtccggatgc aagctgtcga aaaagcgccg
     2161 cacctcggtg ccgaagctcc cgggcccgta gccgagcatc gtgcagaagc ggtcattcac
     2221 atcgagcgtg ccggccggga cattccagtc ccaagtgccc atatcggcgc cggtgatgat
     2281 ggtcgacagc ttatcgcgag attcgcgtat ctcttcttcc gcctgcttgc gctccgtcag
     2341 atcttgaatt agcgcaaaaa acccggctac ccggccattt tcacgatcag gaacatacgt
     2401 agcccggaca taacgctgcc ccgctcctct gtaaggtatg aactcttcga aggtgatcaa
     2461 ctggcccgat aatgcggctt cgactcgggg acggatagcc tcgtaggcct tctctcccaa
     2521 aacctgtcgt aggtgcttcc cttcgacgta ttcgcgcgaa tggccaaacc agtgctcgta
     2581 acctttgttg ttaaaacggt agcgctgggc ttcatccaca taggatatca gcaccggcaa
     2641 ggaatcggtg agaagccgaa accatccttc gcttctgcgg agcgcatgtt cggcctgttt
     2701 gcgttcggta atatccgaat gggtaataac cgcgccggcg cccggctttt tttgccggag
     2761 gacagtcatc ttgaaccatc gttcctgcaa cggcgacgag cactcatagt ccatgacagc
     2821 atggtccttc gtaccgttca gcacggactc gattgcgttc aagacatgct ccgctccttg
     2881 atctcccgcc tttattgcct gccggcaaac ctccaggtag ttcgccccga ccgaaacgca
     2941 cgatgtgtca ggcgcaccgt tttgaactgc gaagttttcc caggcctcgt tgaccgctcc
     3001 aatagttccc tccgcgtcca ccacggcgat gtgatcatgc agtgagtcaa gaattgcgcg
     3061 gacaaattgc ggcgattcct ttgaactcgg tttctgaatc gtaataatcc acctcgcttc
     3121 gcggtaccgc gccttctcca ctgcttcccg gaactttgta gtttgattag tgcaaagggt
     3181 atacccgtaa gtatcggaca actgcccgca aaacaataac tttcctcgcg ttcgtcgccg
     3241 caacttctca gcgcagagaa taccggctct actattctcg ttcttgataa taatgctcgt
     3301 ctgtgcattg gcagaaatta cttccgtcaa ttgggaaaat ttaccgaagt catcagggat
     3361 ttcgaaccta aaactcaggg aatttccgat tacaacgaat cgcagaatcg tgattaaaag
     3421 gttgcgagcg ggaaaactgc cgtcatcaag cagagttcag cagtctcgat cacaaaataa
     3481 cacgtgaccg accaggcatc agagacacga actactaacc gcttcaacgg gaaagctcaa
     3541 cgcgttgcca gtcttggtaa attaaaagtt tcgagcttga agatgcgagc tggatttggc
     3601 gccgattgtt cagactatta ccgacgctag aaatgactta gaggaagttg tgttagaact
     3661 aaaaggaaat gacgcgtcta ctgctgctgt tcgctgtgga attgtgttag cggccgggaa
     3721 tggtaggcga ctgcgggagt ttgtccgcca gcaacgcggt gattgcttgc ccaaacaata
     3781 cattaacttc gtcggcaacc gatccatgct ggagcatacc tgggatcgcg cggaaagctt
     3841 gatcgctgcg cagaaccttt ttatcgttat tgcaaagcaa catctcgaat tccgcgaagt
     3901 ttaccgccag cttgaatcaa agccgccgca gagcttgatc atccaacccg caaataggga
     3961 aacagcgcca ggcgtcttgc ttccattgct ccatatttac aaacgctatc ccaaagctat
     4021 agtcgctgtc tttccgtcgg accatttcgt tctcgaagaa gatctgttta tgcgccatgt
     4081 ggaccaggca ttccgggtgg tcgagtcgga tgcttcccgg atgatccttt tgggcctgga
     4141 gcctcatgaa ccggacccgg aatacggcta tattgttcca gggaaaaggg ttgaaacctt
     4201 cccgggcgac tcggccagac aggtcgagat gttcgtggaa aagccatgca cggaggcagc
     4261 caaaaagatc atttcaagag gagcgttatg gaacacaatg gtgatggtct tcgcttgtaa
     4321 gaccgtcttg tccataatcc aacgcgcggt ccccgagctt tatcactcct ttggccctgc
     4381 tctcgacgct ctcggtacac cc
//
LOCUS       DASUTG010000303         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_47873, whole genome shotgun sequence.
ACCESSION   DASUTG010000303 DASUTG010000000
VERSION     DASUTG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47873"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF089_06935"
     CDS             <1..226
                     /locus_tag="VF089_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173734.1"
                     /translation="HFCSMCGPHFCSMKITQDVRDYAAQKGLEDQAALEAGMQEKAEE
                     FKKTGGKLYPELAGEGAGSAEAGYRAAGKE"
     gene            337..867
                     /locus_tag="VF089_06940"
     CDS             337..867
                     /locus_tag="VF089_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173735.1"
                     /translation="MRVLVYGFGPYREFRNNITEAIIKALPPLPELTTAVFPVRFNRR
                     QFIDMLERHKPDHVLGLGQCMRHRIVFESRATNRKRVSNRVSVKPIRQSGAEFLSTNL
                     DIDLGSDVGHSDSAGDYVCNYSMYVILEYISRSELDIRFGFVHIPHDIDYGRGVALVM
                     QAISQIQLKSERACRK"
     gene            1157..1393
                     /locus_tag="VF089_06945"
     CDS             1157..1393
                     /locus_tag="VF089_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173736.1"
                     /translation="MRGGKHRPFKNAAQAQKIAHRCRTAGKELKRSSGVSSEEDHNLI
                     ELRSKTSTLWKAAGEMGRQLEDKSWERESSKPGV"
     gene            complement(1395..2267)
                     /locus_tag="VF089_06950"
     CDS             complement(1395..2267)
                     /locus_tag="VF089_06950"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX6173737.1"
                     /translation="MIEEALSGLRLFNKGKVRDIYDLGDNLLLVATDRISAFDVILPT
                     SIPGKGKILTALSEFWFGITEDLIPNHFITTRFDQFPAACLPHREKLEGRSMLVKKST
                     PAPVECIVRGYLAGSGWKDYQNTGSVCGIRLPQGLVEASRLEQPIFTPSTKAAIGAHD
                     LNITFDDMLTKLGKKRAEAMRDSSIAIYKRASHLAETRGIIIADTKFEFGLEADKVIL
                     IDEVLTPDSSRFWPVDGYRPGKTPDSFDKQFVRDYLLNLPWDMKSPPPELPPEIVKRT
                     QEKYAEALRRLTAA"
     gene            complement(2355..2651)
                     /locus_tag="VF089_06955"
                     /pseudo
     CDS             complement(2355..2651)
                     /locus_tag="VF089_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
BASE COUNT          681 a          729 c          759 g          677 t
ORIGIN      
        1 tcacttttgt tcgatgtgcg gtcctcactt ctgctccatg aaaataaccc aggacgtgcg
       61 cgactatgcg gcacagaagg gtttggagga tcaggccgct ttggaggcag ggatgcagga
      121 gaaagcggaa gagttcaaaa agaccggcgg caaactctat cctgagctgg ctggcgaggg
      181 cgcagggagc gccgaagccg gctatagggc ggcaggcaaa gaataggcgc aatacttttt
      241 gactgattgc gaaggactgg ccggccaaat gccggccagt tttttttggt cttcatcatg
      301 tacgtggctt cataaagcat ctcacgggat tgagctatgc gcgtcctcgt ctatggcttc
      361 ggaccttatc gagagttccg caacaacata accgaggcaa tcatcaaagc gttaccgcct
      421 cttcccgagc ttacaacggc cgtgttcccg gtgcgattta accggcgcca gtttatcgat
      481 atgctcgagc ggcacaaacc cgatcatgtg ctcggtttag ggcaatgcat gcggcatcgg
      541 attgtgttcg aatccagggc aacaaaccgc aagcgtgtca gcaacagggt ctcggtcaaa
      601 ccgattcgcc aatcgggagc cgaatttcta tcgacgaatc tcgacatcga ccttggctcg
      661 gacgtgggtc attccgacag cgccggagat tacgtctgca actattcgat gtacgtcatc
      721 ctggaataca tcagccgcag cgaactggac atcaggtttg gatttgtaca tatcccgcac
      781 gacattgatt acggaagggg cgtcgctctc gtgatgcagg cgatctccca aattcagctc
      841 aaatcagaac gagcatgccg gaaatagggt cgctgcagtc gctgggatcg agcaataaac
      901 gtgtcggtct tctctgcctc tcgacgatca acagtctaca taagattcct tcaggtcaga
      961 gcatgaattt tacagcttca gggccaattt ggtccaggct atcggcgacg gacctctgac
     1021 caacgtaaaa gcaatcgaat tgtggcgcgg tcagagtgcc aagttgatta cgcgatcggc
     1081 acagttgccg gaatgagctc cggcgaagac gcatcagccc gttgccgaat cacgcatcct
     1141 cccttacgag aaacttatga gaggcggaaa acaccggccc ttcaagaatg ccgcgcaagc
     1201 ccaaaagatc gcgcaccgct gccggacagc gggtaaagag ttgaagagga gcagcggagt
     1261 ttcttccgaa gaagatcaca atctaataga gttgcgaagc aagacaagca cactctggaa
     1321 ggcggccggt gagatgggca gacagctcga ggataagagt tgggagcgag aaagctcgaa
     1381 acccggcgtt tagatcacgc ggcggtaagc cgcctcagag cctcagcata cttctcctga
     1441 gttctcttaa cgatctcagg cggtaactcc gggggtgggg acttcatgtc ccatggtaga
     1501 ttgagcaagt aatcgcggac aaattgttta tcaaagctat caggcgtttt gcctggtcga
     1561 tatccatcca ccggccaaaa acgagacgaa tccggagtaa ggacttcgtc aattaagatt
     1621 accttatcgg cttcgaggcc aaattcgaat ttggtgtcgg cgataatgat cccccttgtc
     1681 tcagccaagt ggctcgcccg cttgtaaatc gcaatgcttg agtctcgcat tgcctctgct
     1741 cttttttttc ccagttttgt cagcatgtcg tcgaaggtga tatttaaatc atgcgcgcca
     1801 atcgcagctt tggtcgaggg cgtaaaaatc ggttgctcca ggcgcgatgc ttccactaag
     1861 ccctgaggaa gccggatacc gcatacgctc ccagtattct ggtagtcctt ccagccggat
     1921 cctgccaggt agcctcgcac gatacattcc accggagccg gcgtgctctt tttgaccaac
     1981 atcgatcggc cctccaattt ctctcgatgt gggaggcaag cggcggggaa ctgatcgaag
     2041 cgggtcgtaa tgaaatgatt agggatgagg tcttctgtta taccgaacca gaattctgaa
     2101 agggcagtta ggattttgcc tttacccgga atcgatgtgg gtaatatcac gtcgaatgcc
     2161 gagatgcgat cggtggcgac taacaagagg ttgtcgccta agtcatagat atcccgtact
     2221 ttgcccttgt tgaacagacg caaaccggaa agagcttctt cgatcatcga gataggcttg
     2281 ctcgctgtta tggacggaaa agattccgga tgggatcggc atcggccggc gacagatcgc
     2341 ctcccgaaaa ctgactacaa aagttcctta acctgatttt cgttgaagcc ggtcagtttc
     2401 tttcttcctt ttataaagac gggacgcttt aacagatttg cgtctttcaa catgagtctg
     2461 atggcttcat ccttggatgg gggcttttgc ttcatgtttt tctcgcgata caggggggtg
     2521 cgggtgttta agaaaatgtc gatgggctca tctccaatga tttcacgcaa ttccttctct
     2581 gaaaacgggt tcttaccgta ctcgcgctct tcgaaagcga tcttgtttgt ttgcaagaaa
     2641 cttcttgctt tgcgacaagt cgttcacgtt gccttataga tgaatttcag cttctcggcc
     2701 atgactcatc cagcggactt acttgaagaa cctggctaca cgttcgaatg cagccggttc
     2761 gacctcgttg cgcaccggcc attccgcctt caaaggaatc gtcgcgtcga taatcgcgac
     2821 agcgtcgccc agccagccgc gaaaac
//
LOCUS       DASUTG010000304         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1382416, whole genome shotgun sequence.
ACCESSION   DASUTG010000304 DASUTG010000000
VERSION     DASUTG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382416"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1151
                     /locus_tag="VF089_06960"
     CDS             225..1151
                     /locus_tag="VF089_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6173738.1"
                     /translation="MAVDSLASQTSQKTKGPLVWLDMDQKQLDDAYNQSVYAPNQEQV
                     AKRRRLASEAAIKRLAPALRLSYGPTEIEKLDIYRTSRPNAPIMVFTHGGAWRNGSAK
                     DFAFPAEVFINAGAHYIVLDFAAIQDVGGNLMVMADQVRRAVAWVYQNAETFGGDRNR
                     LYVAGHSSGAHLTGVILVTDWRRDFDLPADIVKGGLCVSGMYDLKPVRLSARSKYVKF
                     DDLTEQALSSQRHLELLNCPITVGYGSQETPEFQRQGREFAAAIKAAGKPVKLIVGEG
                     FNHFEMQETFGNPYGLAGRAALELMNLQTPST"
     gene            1530..>2596
                     /locus_tag="VF089_06965"
     CDS             1530..>2596
                     /locus_tag="VF089_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX6173739.1"
                     /translation="MPKNDLNRRLRTLAAVLALAVVYFCAGIFGLSLAFTNPNASAVW
                     PSSGIALAVILVWGYRLWPGIFLGAFLVNFATQGSLPTALGIAIGNTSEAILAASLLA
                     RFAQGAHVFNRTINILTFIFLAGILSTAAGATMGAITLYLGGFIARPEFPTVWLTWWL
                     GDAVGNLIITPLLLMWLTQPVLPVRSRHIFEAAGLLAAVVSTSALLFLNPVPAGLEYF
                     ALVPLLWAALRFGRRGAVTAAFVTSGIALWGTVRDLGPFAQSEPHESIFLLQLFIATI
                     STTALIVASVVSQQQRLEQRLRIKDAVSRILSEASTMTEAAPQIIKAICETAAWDFGA
                     LWLVERNSNVLTCVEMWCLPS"
BASE COUNT          548 a          717 c          695 g          636 t
ORIGIN      
        1 agttccaggg tttcaaaagg ctaaacggtg acgtgcttcg attcgcttcc gagcatgaga
       61 gatgcaagtc atcattatca acttactccg ttgctatgac ccggacggac cggggtggat
      121 taagatccgt tgttttctta cgttgaactt tttaactgtt gaaccttgaa caaagtttta
      181 agttgaccta tcgttccggc attaactcag ttcgggggtt aagcatggct gttgattctc
      241 tcgcttcgca aacgagtcaa aaaaccaaag gcccgctagt ctggcttgac atggaccaga
      301 agcaactcga tgacgcgtac aaccaatcgg tttatgcgcc taatcaggag caagttgcta
      361 aacggcgccg attggcaagc gaagcggcaa tcaagcggct tgcgccggcc ctacgtctct
      421 cctacggtcc gactgaaatc gaaaaactcg acatctacag gacctcgcgc cctaacgcgc
      481 ccatcatggt ctttactcac ggcggcgcct ggcgcaacgg atcggcaaaa gactttgctt
      541 ttccggcgga ggtattcata aatgccggag cccattacat cgtcctcgac ttcgccgcga
      601 tacaagacgt cggcggcaat cttatggtca tggcggatca agtccggcgc gcagtcgcat
      661 gggtttacca gaatgcggaa acctttggcg gcgatcgcaa ccgcctttat gtagccggcc
      721 actcctcggg cgcgcatctc accggcgtca tcctggtgac cgattggcgc cgggacttcg
      781 atttacccgc ggatattgtc aagggcggat tatgcgtcag cggcatgtat gatttaaaac
      841 cggtacgact gtcggcgcgc agcaagtatg taaaattcga cgatctcacc gaacaagcgc
      901 tcagctcaca gcgacatctc gaacttttaa attgtccgat caccgtgggt tacggctccc
      961 aggaaacacc ggagttccag cgtcaaggcc gcgagtttgc cgccgccatt aaagctgccg
     1021 ggaagcctgt gaaattgatc gtcggtgagg gcttcaatca tttcgagatg caggagacct
     1081 tcggcaatcc ctatgggctg gccggtcgtg ccgcactgga actgatgaat ttgcagacgc
     1141 cctcgaccta gcacgatagc cgtgcagtaa cttgttgcgc aattggagag ctgaaaaact
     1201 ccgcgagatt gccttgtcgg ggaattacaa gtcgcgtggc gaagggcacg cttcgtaagg
     1261 cgaagccgtc ccaactggat ctcgtgcctg cataggtgat gtcaatcgac ctatggcatc
     1321 tatcaactat caacactgcc cgactacagc accgatgctt ctttcccgga aaatttttaa
     1381 gcgggtggtg atttatcggc cggtggattg aaattattca caacaaaaat tattcacagc
     1441 aagaggtgtt cattaaacac atctgatccg ctatcatagg cgatccctag ttccctacag
     1501 ctcaggtccg gagctagtaa gagccggtga tgcctaaaaa cgatctgaat cgcaggttgc
     1561 gtacattagc tgcggtcctt gcgcttgccg tcgtttactt ttgcgccgga attttcggcc
     1621 tgtcgttagc ttttaccaac cccaacgctt cagcggtatg gccatcgagc ggcattgcgc
     1681 tagccgtgat cctggtttgg ggttaccggc tttggccagg cattttcctc ggtgcatttc
     1741 ttgtcaactt cgccacacaa ggatcacttc cgacggcgct cggcatcgcc atcggcaata
     1801 cgtccgaagc gattctggca gcgtcgttat tagctcgctt tgcccagggc gcccatgttt
     1861 ttaaccgcac cataaacatc ctcaccttta tatttctcgc cggcatcctc agcactgctg
     1921 ccggcgccac gatgggcgcg attactttgt atctcggtgg cttcattgcg cggcccgagt
     1981 ttccgacggt ctggctgaca tggtggctcg gcgatgcggt gggaaatctg atcatcactc
     2041 ctctgctgct gatgtggcta acacaacccg tattgcctgt tcggtccagg catatcttcg
     2101 aagctgcagg tttgttggcc gcggtggttt cgacgtcggc gctgctgttt ctcaatccgg
     2161 ttcccgctgg cctggaatat tttgctctcg tgccgctgct ttgggccgcc ttgcgctttg
     2221 gccgaagagg cgccgtcacg gcggcattcg tcacatcagg gattgcgctc tggggcacgg
     2281 tgcgtgatct cgggccgttt gcgcagtcgg agccgcacga atcgatattt ttgctacagc
     2341 tttttatcgc tacgatcagc acgacagctc tcatcgtcgc ctcggtggtg tcgcaacagc
     2401 agcgtttgga gcaacgcctg agaataaaag acgccgtgag ccgcatatta tccgaagctt
     2461 cgacgatgac cgaggcggcg ccgcagatca tcaaggcgat ctgcgaaacc gcagcctggg
     2521 atttcggcgc actatggctc gtcgagcgaa attctaacgt actcacttgt gtcgagatgt
     2581 ggtgcctacc ttctat
//
LOCUS       DASUTG010000305         5154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_641184, whole genome shotgun sequence.
ACCESSION   DASUTG010000305 DASUTG010000000
VERSION     DASUTG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641184"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF089_06970"
     CDS             complement(<1..333)
                     /locus_tag="VF089_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173740.1"
                     /translation="MMPKSSPIVAQKSFKYRRWKARLLAVAAIFLFGAVSSTGSHAQT
                     PYYQGKTITLVVGTVAGDLYDLYARAFAQHMPRHIPGSPNMIVQNMPGAGHMIAANYL
                     YSVAKPDGL"
     gene            complement(354..1469)
                     /locus_tag="VF089_06975"
     CDS             complement(354..1469)
                     /locus_tag="VF089_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173741.1"
                     /translation="MALFFHSDEVDEFIPFAEAVAISESALRDLKTPAGVNAPRKRLH
                     LHRDIAEGSFDTVLNIYAGGSAGYGAIGAQVALHRKAIVGTMQRRPPYNPDQTELALI
                     YDTDTGSLLGVMAHRPRHVGGAADLRTPAVSLAGLDRFAKRDAKRVGIYGSGQQAYST
                     FVGLTEMRRIESAKVYSPTPEHREGFAKKINQRTGVAVRTVENPELAARDVDIVLCMT
                     NTNVPVLNGSWLEEGQYIISVVGSNIELVKSGNIAAPRREVDDETLKRCSFIVALSKE
                     QAVDSQQGDIYWPVQNGVIAWDKVIDISDVLTGKAKGRSDDKQIILYKNQGGQGIVDI
                     ALAKRCYDLAKQHGKGYELRIEARENWWVQGGRTQTW"
     gene            complement(1480..2481)
                     /locus_tag="VF089_06980"
     CDS             complement(1480..2481)
                     /locus_tag="VF089_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173742.1"
                     /translation="MQYRMIGKTGLRVSEIGFGCGNNAVLMVKASYEDQVQAVRHALD
                     LGINYFDTAYAYGLGKSEENLGRILNQLNAPAVISTKIRLEADALTDIKAATIREVEA
                     GLERLQRDSVDLIQLHTRVLTSRQQNQRFGMTPGEILGRSGVLEGFKAMRDKGKVGYF
                     GFTGLGDPAALHEVVDSGAFHSFQAYYNLLNPSAGHPVPSAFSALDYGLLIDRAAAQS
                     MGVAVIRVLAAGALTSNPGAGGGSTPEPLSKGSEYSLDVERAEKLKAALAVDGKGMTQ
                     IAIRFGLMNPKVSTVLVGFSNTAHIDEAVACSGGPPLSNEETAKIKKLWQTDFGKLN"
     gene            complement(2633..3325)
                     /locus_tag="VF089_06985"
     CDS             complement(2633..3325)
                     /locus_tag="VF089_06985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6173743.1"
                     /translation="MKRLLLFDIDGTLTRSHNGHVPFNQAILQTFGVPGDILSVIPDG
                     NTDPMIVTEIFTKSNILIHDIDDKWEQFSSNLHQCYSQAISSGITTVRALAGAVQLLQ
                     AVAASGILGSSVVSGNLEPMARVKLEAAGLYSYLCRGAYGSDSPRRADLPAIAKHRCE
                     AALGVSIPLSDCIVIGDTPKDFEAARCNHMKCILVATGRYSLEELSKCKPELCLPDLT
                     DTGAVLETLLSI"
     regulatory      3430..3513
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            3684..4826
                     /locus_tag="VF089_06990"
     CDS             3684..4826
                     /locus_tag="VF089_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6173744.1"
                     /translation="MIKHYLLSPGPTPVPNEVALAMSETMIHHRTPQFNKVFDQARQG
                     LKKLFATKNDVLMLASSGTGAMEASVANLFSPGDRVLVINGGKFGERWLNIANAFGLN
                     PVELKVEWGQAVRVDAVEKQLKAHPDLKGVMIQASETSTTVLHPVREIAKLTKNGPLF
                     LVDGVTAVGVVSIPLDEWGLDVLVTGSQKALMLPPGLGFIALSDRAWERTKQATLPRF
                     YFDLNLERKNQQKGSGAFTPAVSLIFGARASLEMMQREGLDRVYARHARMSRATRAAA
                     TALGLKLLAPDNPSPAATGVYLPDGIDADQVLDYLRDHMNITLAEGQDQLKGKVIRIA
                     HVGYMGAFDVITAVAALEMVLRKFGVELPFGKGVAAAQEVLMESLV"
     gene            4829..>5154
                     /locus_tag="VF089_06995"
     CDS             4829..>5154
                     /locus_tag="VF089_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6173745.1"
                     /translation="MKILVSDSLAPQCLEVFKRAPGFEVDVRIGLDAKELKKICADYD
                     GWVIRSGTKITRDLIEAAKNLKVIGRAGVGFENIDVDAATQRGIVVMNTPGGNNVTTG
                     EHTISL"
BASE COUNT         1146 a         1409 c         1394 g         1205 t
ORIGIN      
        1 taagccgtcc ggtttggcca cgctataaag ataattggcc gcgatcatat gacccgcgcc
       61 gggcatattc tgcacgatca tattggggct gcccgggata tgcctaggca tgtgctgggc
      121 aaaagcgcgg gcgtaaagat cgtaaaggtc tccggccacg gtaccgacga caagcgtgat
      181 ggttttcccc tgatagtagg gtgtctgagc gtgagagccc gtgctgctta cagcgccaaa
      241 aagaaaaata gcagcgacgg caagcagccg agctttccat cttcggtatt tgaaactctt
      301 ttgggcaacg atcgggctcg atttcggcat cattggttgt actcctgtcg gatttaccac
      361 gtttgagttc ttcctccctg cacccaccag ttctcgcgcg cttcgatccg aagctcgtaa
      421 cccttgccgt gttgttttgc aagatcgtag caccttttgg ccagagcaat atcgacgata
      481 ccctgcccgc cttgattctt gtacagaatg atttgtttgt cgtcgctgcg gccttttgct
      541 tttcccgtaa ggacatcgga gatgtcgatt accttatccc aggcgattac tccattttgc
      601 acgggccaat aaatatcgcc ttgctgcgaa tcgacagcct gttcttttga cagcgccacg
      661 ataaaactac agcgtttgag tgtttcgtcg tcaacctcgc gccggggcgc agcgatattg
      721 ccgcttttca ccagctcgat gttgctgccg accaccgaga taatatattg cccctcctca
      781 agccacgatc cattgagaac cgggacatta gtattggtca tgcacaaaac aatatcgaca
      841 tcgcgggccg caagctctgg attttctacc gtgcgaacgg ctacaccggt tctctgattg
      901 attttttttg caaaaccttc tctgtgttcg ggcgtgggac tataaacttt ggcgctctcg
      961 atccgtctca tctcggtcag tccgacgaag gtcgaataag cttgttgtcc ggagccgtaa
     1021 atgccaacgc gcttggcgtc acgttttgca aaacgatcca ggccggccaa acttaccgct
     1081 ggcgtgcgca gatcggcggc gccgccgaca tgacgcggcc gatgcgccat cacccccagc
     1141 agggatcccg tgtccgtgtc gtagattagc gcaagctcgg tttgatcggg gttgtaagga
     1201 ggtcgccttt gcatagttcc gacaatcgct tttcgatgga gcgccacctg cgcgccgatt
     1261 gcgccgtagc cggcagagcc gccggcataa atgttgagca cggtatcgaa gctcccttcg
     1321 gcaatgtctc ggtgcagatg gagccttttg cgcggcgcat tgaccccggc aggagtcttc
     1381 agatctcgaa gagcactttc agaaatggcg acagcttcgg caaacgggat gaattcatcg
     1441 acttcgtcgg agtggaagaa cagcgccatt ttagcctcct tagttcaatt taccgaaatc
     1501 tgtctgccaa agctttttta tcttcgccgt ctcttcgtta gacaagggcg gcccgccgga
     1561 gcatgccacc gcttcatcga tatgggccgt attggaaaaa ccaaccagca ctgtcgaaac
     1621 ttttggattc atcaatccaa agcgaatcgc gatttgcgtc attcctttgc cgtccacagc
     1681 aagagccgct ttcaactttt cggcccgttc tacatcgagc gagtattccg atcctttaga
     1741 cagcggttcc ggggtactgc cgccgcccgc gcccggattg gacgtgagcg cgccggcggc
     1801 taaaacacgt atgaccgcaa cccccatgct ttgggcggcg gcgcgatcga tcagcaggcc
     1861 ataatccaaa gcgctgaatg cagacggaac gggatgaccg gcgctgggat tgagcagatt
     1921 gtaataggcc tgaaacgagt ggaaagcgcc gctatcgaca acctcgtgca aggctgcggg
     1981 atctcccaaa ccggtaaaac caaaatagcc gacctttcct ttatcacgca tcgccttgaa
     2041 accttcgagc acgccggagc ggcccagaat ttcgccgggt gtcatgccaa agcgctggtt
     2101 ttgttgccgg ctggtgagaa ccctcgtgtg tagctgaatc agatcaacgg aatccctctg
     2161 caaacgttcc agcccggctt cgacttcgcg tatggttgca gccttgatat ccgtcagtgc
     2221 gtcggcctcg agccgaattt tcgtcgagat tactgctgga gcgttcagct gattgagaat
     2281 tcttcccagg ttctcttcgg attttcccaa tccataggca tatgcggtat caaaatagtt
     2341 aataccgaga tcaagcgcgt ggcgcactgc ctgaacctgg tcttcataac tcgccttgac
     2401 catcaacacg gcattgttgc cgcagccgaa gccgatttcc gagactcgaa gtccggtttt
     2461 gccgatcatc cgatattgca taatgacacc tccctaacat agcaggttct gaaactctga
     2521 tgcgcgctgc tccatcggcc gcttcttctc gttttcggct cgttgggaat tcgatcagtt
     2581 ttcgacaaac acgagcacga gatgcgttcc cggctcaaac ttgaggagcc tgctagatgc
     2641 tcaaaagcgt ctctaaaacc gcgcccgtgt ccgttaaatc cggtaaacaa agttccggtt
     2701 tacactttga taattcttct agcgaatacc ggccggtagc gacaaggatg catttcatgt
     2761 gattacatcg ggcagcctcg aaatccttcg gagtatcgcc gatgacaatg cagtcgctga
     2821 gcggtatcga cactccgagc gccgcttcgc agcgatgctt cgcgattgcc ggcaaatcgg
     2881 cgcggcgcgg cgaatcactg ccgtaagcgc cgcggcacag ataggaataa agaccggctg
     2941 cttcaagctt gactcgggcc atcggttcca gattgccgct gacgacactg ctgcccagta
     3001 tgccgcttgc ggcgaccgcc tgcagcaatt ggacggctcc ggctaacgcg cgcaccgtag
     3061 taattccgga gctaatcgcc tgtgaatagc attgatgcag gttgcttgag aattgttccc
     3121 atttatcatc gatgtcgtga atcaggatat tacttttggt gaaaatctcg gtgacaatca
     3181 tcgggtcggt attgccatcg ggaatgaccg agagaatgtc tcctggaacg ccgaaggtct
     3241 ggagaatggc ttggttaaag ggcacgtgac cgttgtggct tcgagtcagg gtcccgtcga
     3301 tgtcgaagag taaaagtcgt ttcatggagc atcggatagt cgcaaatcga gccggcaatg
     3361 gcaaattgcg gcgtccgcag cgatctcaaa ccgttgacaa gctgtagggt gtgcggtaaa
     3421 caactcatgt aagttttgcg cgactggcgt ctacgtggaa ttgaccacga gggagtgcaa
     3481 agcataagac ggccgaacgc ctgggtctct tacggaggag gttcaggctt ttttatgtcc
     3541 tgcgaacata cttcgtttat cgcgcatcac tttcgctgcg gtcaacaatt ccagctcgac
     3601 cttgaggtca cgtctcgttc tgctcgttcc atttcccggc aaatcgccgg ggctgctaga
     3661 gcttcgagag gataaataaa tccatgatca agcattattt actttcgccg ggtccgacgc
     3721 cggttcccaa cgaggtggcg ttggcaatgt cagagacgat gatccatcat cggacaccgc
     3781 agtttaataa agtcttcgat caagcgcgcc agggactcaa aaaacttttc gccaccaaaa
     3841 atgacgtttt gatgcttgcc tcttccggca ccggcgccat ggaagcatcg gttgccaact
     3901 tattttcccc cggtgataga gttctggtca tcaatggcgg gaaattcggc gagcgttggc
     3961 ttaacatcgc caacgccttc ggcttgaatc cggttgagct caaagttgaa tggggtcagg
     4021 ccgtaagagt tgacgccgtc gaaaaacaac tgaaagccca tccggatctg aaaggtgtca
     4081 tgatccaggc cagcgaaacc tcgacgacgg tgcttcatcc ggtcagggaa atcgccaagc
     4141 tgaccaaaaa cggcccgcta tttctcgtcg atggggtcac agcggtgggc gtcgtttcga
     4201 taccgctcga tgaatggggc ctcgacgttc tagtgacagg ctcgcagaaa gccctgatgt
     4261 tgcctcccgg ccttggtttc atcgccctga gcgatcgcgc ctgggaaaga actaagcaag
     4321 caacattgcc acgattttat ttcgacctga atctcgagcg caagaaccag caaaaaggtt
     4381 ccggcgcgtt tacaccggca gtgtcgttga ttttcggcgc gcgcgcgtcg ctcgagatga
     4441 tgcaacgcga aggtctcgat cgggtctatg cccgacatgc gcgcatgtca cgcgccactc
     4501 gagcggcggc cacggcctta gggttaaagc tgctggctcc cgataacccc agtccagcgg
     4561 ctaccggcgt ttacctgccg gatggcattg atgccgacca ggtgctcgac tacttgcgcg
     4621 atcacatgaa catcaccctc gccgaaggac aagatcagtt aaaaggaaaa gtcattcgca
     4681 tcgctcacgt cggttatatg ggagccttcg acgtgatcac cgcggttgcg gcgctggaga
     4741 tggtcctgcg caaattcggc gtcgagcttc cgttcggtaa aggcgtcgcc gcggcacagg
     4801 aagttctcat ggaatctttg gtttgaagat gaagatccta gtgagcgatt ctctcgcgcc
     4861 tcaatgcctc gaggtattta agcgcgcgcc gggttttgaa gtcgatgtgc gtatcggcct
     4921 cgatgcgaaa gagctaaaga agatttgcgc ggattacgat ggttgggtga tccgaagtgg
     4981 aacgaagatc acccgcgact tgatcgaagc ggcgaagaac ctgaaagtga tcggacgcgc
     5041 cggcgtcggt ttcgaaaaca tcgacgtcga tgcggcaacc caaagaggca tcgtcgtcat
     5101 gaatacacct gggggcaaca acgtcaccac cggggaacat acgatttcac tgat
//
LOCUS       DASUTG010000306         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_196354, whole genome shotgun sequence.
ACCESSION   DASUTG010000306 DASUTG010000000
VERSION     DASUTG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196354"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VF089_07000"
     CDS             complement(<1..261)
                     /locus_tag="VF089_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6173746.1"
                     /translation="MAALTLEMAEKAVKAAQAKARDQGTPMTVSVVDEAGRLVLSARG
                     DGAGFFTPETSRAKAVAAAAFRRPTKEMVRLRDSSPAFWSNIS"
     gene            694..1635
                     /locus_tag="VF089_07005"
     CDS             694..1635
                     /locus_tag="VF089_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6173747.1"
                     /translation="MKRVTLYSSSRKSITGQAPPTKQLEQPQAPDQSRQTAAQSSKPS
                     PRTRWRDFYVRNERRFLGAAAAGLLSILLVFTYSALKPPREITQEDIRAAVLHTLETN
                     TLPSPVARAYDVIRSSVVRVNGWGAEDAIDDDSNKSVGSGVVIVDKGTILTNLHVVLG
                     AKRVQVVFADGLKSDATVIAMQPEHDLAVLQAETIPDDLVAATMRSTADLALGDQVVA
                     VGFPFGIGPSVSAGVVSGLRREFRSPEGKRLLTNLIQFDAAVNPGSSGGPLVTAGGEV
                     VGIVTAILNPTDQRFFVGIGFAVPIENAAAAAGLPPF"
     gene            1665..2666
                     /locus_tag="VF089_07010"
     CDS             1665..2666
                     /locus_tag="VF089_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6173748.1"
                     /translation="MDNKEIYRGEIASLMERILYEVKKVIVGQDHFLERVLVAVLAQG
                     HLLVEGVPGLAKTLTVKTLARTMRGSFRRIQFTPDLVPADLVGTRIYNQKSGDFATSL
                     GPVFTNLLLADEINRAPAKVQSALLEVMQERQVTIAGESHYVPEPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVGYPTEEEEFVIVARVTGTANDVVPVATTDQLNALRRDCQ
                     AVYVDPSLMQYAVRLASATRDPDRFGLPDLTKFVSFGASPRASIHLIEGARALAFLRG
                     RGYVLPEDVMDLVSDVFRHRLVLTYEALAEGMTADQLIHRIMQQIPAPDKPLETHVR"
     gene            2815..3828
                     /locus_tag="VF089_07015"
     CDS             2815..3828
                     /locus_tag="VF089_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6173749.1"
                     /translation="MSVFQKPPKESPGAASEDFRAPVARNSGAENILRRLEWTVIRRL
                     DGLLHGDYQTLFRGFGLDLADLREYQYHDDVRYIDWNVTARLQTPYIRVYNEDREVTA
                     WFLLDLSPSVDFGSKTRKQSVSTDFVTMLARLLTRHGNRVGALFYGDRVDTVIPARTG
                     RRHVLDILHRVLSRPKRSDSHGTNLRDLFQTASRIMNRRSLVFVISDFISEPGWAKPL
                     AQLSQRHEIIAVRLYDPLEMELPDLGLLVMQDAETGEQLFVDTHDRGFRRRFAAAAER
                     RETELRAAFSQAGVDTLELSTNDDLVDAILRFADLRKRRSQLAAGGSLPKHLRRSHDL
                     SMA"
     gene            3809..>4238
                     /locus_tag="VF089_07020"
     CDS             3809..>4238
                     /locus_tag="VF089_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6173750.1"
                     /translation="MTFLWPEMLLLLLLVPVLVGAYFFVLNRKKRAALRYASLSIVKD
                     AMSARQRFRRHIPPLLFLIAITAMIFAIARPAALVTLPSQHRTIILAMDVSGSMRAAD
                     VQPNRIAAAQAAAKAFVADQPSNVRIGVVSFAGTASIVQMP"
BASE COUNT          888 a         1193 c         1205 g          952 t
ORIGIN      
        1 ggaaatattg ctccagaaag ctggggacga atctctaagg cgcaccattt ctttggttgg
       61 tcgtcgaaac gccgctgctg caaccgcttt ggcgcgggaa gtttcaggag taaaaaagcc
      121 cgcgccatca ccgcgcgccg ataagaccaa acggccggct tcatcgacaa cgctaaccgt
      181 catcggcgtt ccttgatcgc gcgctttcgc ctgcgccgct ttgaccgcct tttctgccat
      241 ttccagggtt aatgcagcca tatctcactc ctttctaagt tcaattcggt tttcgatgca
      301 cagcctccta gaggaatcgt gataacgagt gaaatgaaag gagaacattc caactacacc
      361 tcgagcacat ccaatggctt tctgaatctg ctgacaagct tgcagccaga gaatttttcg
      421 atgtaaagcg gtgatggagt tcgaggatag ctttctcctg gtcattttcg gaggcagcac
      481 acaagtggcc gccagctact cttctcgaag gcgaaactat tcgataggct gttgctatgt
      541 caacttactt ttacttgcgg cagagctaac aaagggcttt tatatcaggc ggccttggga
      601 tagatagaat cagtaaaagt tggtggttgc tgccgcagca gaatttgagg tagctcttgt
      661 agatatttct ccctaacctg atggatcgca caaatgaaga gagtaacgct ttacagctct
      721 agccgaaagt cgatcactgg acaggcacct ccgaccaaac agctcgaaca accgcaagcc
      781 ccggatcaat cgcgccaaac cgccgctcaa tctagcaaac cgtcacctcg cacacgatgg
      841 cgagactttt acgttcgcaa tgagcggcgc tttttagggg ctgctgccgc cggattgcta
      901 tcgatccttc tggtgttcac gtattcggcg ctcaagccgc cacgcgagat cacccaagaa
      961 gatatccgcg ccgcggtgct tcacacgctt gagaccaaca cgcttccgtc gcccgtggcg
     1021 agggcctacg acgttattcg ttcctcggtt gtcagagtga atgggtgggg agccgaggat
     1081 gcaatcgacg acgacagcaa caagagcgtt ggcagcggcg tcgtcatcgt ggataaggga
     1141 acgatcctca ccaatcttca cgtggtgctc ggcgccaagc gcgtgcaggt ggtgtttgcc
     1201 gacggtctca aatccgacgc gactgttatc gccatgcagc cggagcacga tcttgcggtg
     1261 ctccaggcag agacgatacc cgacgatctg gtcgcagcga cgatgcgatc gactgcagac
     1321 ctcgcgttag gcgatcaggt agttgccgta ggatttccat tcggcatcgg tccttccgtc
     1381 agcgcgggag tggtgtcggg cttgcggcga gaatttcgct ctccggaagg caagcgcttg
     1441 ctgactaatt tgatccagtt cgacgcggca gtaaatcccg gaagctcggg cggcccactg
     1501 gtgaccgcag gcggcgaagt ggtcggcatt gttaccgcca ttctcaatcc tacagatcag
     1561 cgtttctttg tcgggattgg ctttgccgtt cctatcgaga atgcagcagc ggcggcggga
     1621 ttgccgccct tttgatcgta gatcacttta aactggcagg caccatggac aacaaagaga
     1681 tttatcgcgg tgaaatcgct tcattgatgg aacgaatcct ctacgaggtg aaaaaagtta
     1741 tcgtcggtca ggatcatttt ttagagcgcg tgctggttgc ggtgttggcg caggggcacc
     1801 tgctcgtcga aggcgtgccg gggctggcca agaccttgac ggtcaagacc ctggccagaa
     1861 ccatgcgggg ttcattcagg agaattcaat ttacgccgga tctggttccg gccgatctgg
     1921 tgggaacccg gatctacaac cagaagagcg gtgatttcgc cacatccctg ggtcccgttt
     1981 tcaccaatct tctgctcgcc gatgagatca accgggcgcc ggcgaaagtg caaagcgcgc
     2041 tgctcgaagt gatgcaggaa cggcaggtta cgatcgccgg cgagagccat tatgtccccg
     2101 agccgtttct ggtcatggcg acgcaaaatc ccatcgagac cgaaggaacc tatccgctgc
     2161 cggaagcgca ggtcgatcgc tttatgatga aggtgctggt cggctatccg accgaggagg
     2221 aggagtttgt aatcgttgca agagtcaccg gcaccgccaa cgatgttgtc ccggtggcga
     2281 cgacggatca actgaacgcg cttcggcgcg actgccaggc cgtctatgtg gatccctcgc
     2341 ttatgcaata tgcggtgcgc ctggcatcgg cgacgcgcga cccggatcga tttggcttgc
     2401 ccgatttgac taagttcgtc tccttcggcg caagcccccg ggcctcaatc catctcatcg
     2461 aaggggcgcg ggcgctggcg ttcctgcgcg ggcgcggcta tgtcttgccc gaggatgtca
     2521 tggatctcgt gtccgatgtt tttcgtcacc gtctggtgct gacttatgag gcgcttgcag
     2581 agggaatgac cgccgatcag ttgatccatc gaatcatgca acagataccg gcgccggata
     2641 agccactgga gacccatgtc aggtaagacc aggcgcgtca gcggaacgaa tgcctggaag
     2701 ctgatcgtca aatctaagcg aacccgcaat aggggcacga gcttagagat atcaatgcgg
     2761 ggcgctggaa gcgagttccg tttccggctt gcatgattca tcttctccaa agccatgagt
     2821 gtttttcaga aaccgcctaa agagagtccc ggcgccgcca gcgaagattt ccgtgcccct
     2881 gtagctcgca actccggagc tgaaaacatt ctgcggcgct tagaatggac ggtgattcgc
     2941 cgcctcgatg gactgttgca cggcgattat caaactttgt ttcgcggttt tgggttggat
     3001 ctggcggatc tgcgcgagta ccaatatcac gacgatgtcc gttacattga ctggaacgtc
     3061 acagcgcgtc tccagacacc ttatatccgg gtatacaatg aggaccgaga agtgaccgcg
     3121 tggttcctcc tcgacctgag cccgtcggtg gatttcggct ccaagactag aaagcagtcg
     3181 gtctcaacag atttcgtcac catgttggcg cggctcttga cccgacatgg caatcgcgtg
     3241 ggggcactgt tctatggtga tcgcgtggac acggtgattc cggcgcggac cggccggcgc
     3301 cacgtgcttg atattctcca tcgcgtgctt tcccggccca agcgttccga ctcgcatgga
     3361 accaacctgc gcgatctttt ccagaccgct tctcgcatca tgaatcgacg atcgcttgtc
     3421 ttcgtgattt ccgactttat tagcgaaccg ggctgggcca agccgctggc ccaattgtcg
     3481 cagcggcatg aaatcatcgc ggtacgactt tacgacccat tggaaatgga attacccgat
     3541 ctcgggctgc tggtgatgca ggatgccgaa acgggagaac agctattcgt cgatactcac
     3601 gaccgcggtt ttagaaggcg ctttgcggcc gccgccgaac gccgtgaaac cgagcttcgc
     3661 gccgctttta gccaggccgg cgtcgacact ttggagttgt ccactaacga tgatcttgtc
     3721 gatgccatcc tccggtttgc cgatctgcgc aagcgccgca gccagttggc ggccggtgga
     3781 agcctgccaa aacatttaag gaggagccat gacctttcta tggcctgaaa tgctgctgct
     3841 tctcctgttg gtgccggttc tggtaggcgc atacttcttc gtcttgaatc gcaagaagcg
     3901 ggctgcgctc agatacgcca gcttgagtat cgtcaaagac gcgatgagcg ccaggcaaag
     3961 attccgccgt cacattccac cgctgttgtt tctgattgcg atcactgcga tgattttcgc
     4021 gattgcccgt cccgcggcac tcgtcaccct gccatcgcaa caccggacta ttattctcgc
     4081 catggatgtc tcgggcagca tgcgcgctgc cgacgtgcag cccaaccgga ttgccgcggc
     4141 acaggcggcg gcgaaagcct tcgtcgcaga tcaaccctcc aacgtgcgca tcggcgtggt
     4201 gtcctttgcc ggaaccgcat ccatcgtgca gatgccga
//
LOCUS       DASUTG010000307         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_48593, whole genome shotgun sequence.
ACCESSION   DASUTG010000307 DASUTG010000000
VERSION     DASUTG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48593"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /gene="dnaA"
                     /locus_tag="VF089_07025"
     CDS             <1..1227
                     /gene="dnaA"
                     /locus_tag="VF089_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6173751.1"
                     /translation="NKFFRDWLSEHYFPQISEVLESLTHKELKISLVIDNQSNQSSAA
                     RTEKGPRADKKEEREPNTKHPSRANNLVPKYTFENFVVGASNQFAHAASMAVANQPGD
                     HYNPLFIYGGVGLGKTHLANAIGHRAAATRPGLKIFYLSSESFMNELIGSLRRDKMDE
                     FKTKFRNIDILIVDDVQFIAGKERTQEEFFHTFNSLYESHKQIVITSDKFPKEIPGLE
                     DRLRNRFEWGLIADIQPPDIETRVAIIQKKAEGEGIQLPHDVAIFLATNIESNVRELE
                     GSLTRLGAFSSLTKSLMTVDLAKEVLHNTLKGAKKEITVESIQKTICDFYNLKIADLK
                     AKRRTKNIALPRQVAMYLCRKYTETSFPAIGDKFGGRDHSTVIHASKTIEKRIKEDPY
                     MQNTIERLERNLNLNK"
     gene            1454..2560
                     /gene="dnaN"
                     /locus_tag="VF089_07030"
     CDS             1454..2560
                     /gene="dnaN"
                     /locus_tag="VF089_07030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6173752.1"
                     /translation="MEIKAKRGDLLATLYWTQSIVERRNTMPILANVLLESQKKDIRV
                     TATDLEVGVRGSLEGDVVKEGSVTVNAKKLYEIVREVPNDHIQLKRLENDWVEIRSGK
                     SVFKIVGMDAREFPQFPKFNRDGLTTTPASTLREMIEGTIFSVSTDETRYSLNGVYVE
                     QGEAGNVRLVATDGHRLAFDERNLGSFGLAKGVILPRKGLAELKKLLESGDDGVVSIG
                     FRENMGLVMKDRVELFMRLIDGDFPDYTKVIPKGNQNIARIEHDEFLQALRRVSIISS
                     ERYKGIKMEFNEDKLSISANNPDLGEATEEVEMEYKGKPISIGFNARYLIDVLHVLGT
                     DGEVQIELKDELSPSLVKKGGKEGYLYVLMPMRL"
     gene            complement(2615..3478)
                     /locus_tag="VF089_07035"
     CDS             complement(2615..3478)
                     /locus_tag="VF089_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX6173753.1"
                     /translation="MDKAEMIRRSLKKSGQLVVPGVYDALSARIATKTGFEVIFISGY
                     SLAATMLGEPDFGILTQTEMLSAAQRICSVTALPVIVDADTGYGNAINVIRTVDELIR
                     IGAAGMFLEDQVWPKRCGHMKGKQVIPLEEQLKKLQAAIDAKRNRDFFIVARTDARQA
                     LGLNEAITRGIAFKKAGADAVFIEAPETKEEMLEIARCVPGPLVANMLERGVTPLMGP
                     QELCDLGFDLVVWPLAPLYSVAKSLNEVYTTLRRDGTTVDILDRLMPFDEFNRIVGLE
                     EKYQLDEKYRS"
     gene            complement(3554..3919)
                     /locus_tag="VF089_07040"
     CDS             complement(3554..3919)
                     /locus_tag="VF089_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173754.1"
                     /translation="MFGKGVHQEEEALLREIIRTIDKKMDYSAREGEGSRFTLHFTAR
                     GHDAVVSLDLEDLKAALRDMTRRNQIRQKIKARYDHLDQSRYGADILGLKSARLLRAA
                     PKPEPGRQQRGFGRGGPRR"
BASE COUNT         1119 a          959 c          990 g         1000 t
ORIGIN      
        1 aataaatttt tcagggattg gttgagcgaa cattactttc ctcagattag tgaagtgcta
       61 gaatctttga ctcataaaga gctaaagatt tctctcgtta tcgataatca atctaatcaa
      121 tcttccgcgg cgcgaacgga aaaaggtcct cgagccgata agaaggaaga acgcgagcct
      181 aataccaagc atccctcgcg agccaataat ttagttccaa aatacacatt cgaaaacttt
      241 gttgtcggcg ccagcaatca atttgctcat gcagcgtcga tggcagtggc gaaccagcct
      301 ggcgatcatt acaatccgtt gttcatctat ggcggggttg ggctgggtaa aactcatctt
      361 gccaatgcga tcggccatcg cgctgctgcg actcgtccag ggctaaagat tttttatctc
      421 agttcagagt ccttcatgaa cgaactgatt ggatcgctcc gacgcgacaa aatggatgaa
      481 tttaagacca aatttagaaa tatcgatatt ttaatcgtcg atgatgtgca gttcatcgcc
      541 ggcaaagaga gaactcagga agagtttttc catactttca actcgcttta tgaatcgcac
      601 aagcagatcg taatcacctc cgacaaattt cctaaagaaa ttcccggact cgaagatcgg
      661 ctgcggaacc ggtttgaatg gggtctgatc gcagacattc aaccgccgga catagagacg
      721 cgagtggcga tcatccaaaa aaaagcggaa ggagagggga tccaactgcc tcacgatgtg
      781 gcaatttttt tggcaactaa tatcgaatcc aatgtcagag aattagaagg atctttaaca
      841 cggctcggag cattttcatc tctgaccaag tcgttaatga cggtcgatct tgccaaggaa
      901 gtactccaca acaccctcaa aggtgcaaag aaggagataa cggtcgagag cattcagaaa
      961 acaatatgcg atttttacaa tctaaagatc gctgatttaa aggcgaaacg aagaacaaaa
     1021 aacatcgccc taccacgcca agtcgcgatg tatctttgtc gaaaatatac agagacctct
     1081 tttccggcca tcggggataa gttcggcggt cgcgatcatt cgaccgtgat tcatgcatct
     1141 aagaccatcg aaaagcggat caaagaagat ccctatatgc agaacacgat cgagcgatta
     1201 gaaagaaact taaacctcaa taagtagctg tggataagct gtgcctggcg aacagaacaa
     1261 ttgtgcacaa ctgggaataa ccatcggatg cgctattctc gccaacttag cgcggttaac
     1321 gctacgaatt cacacacagg ctaagatact atagttgctg gtcttttggg gcttgtgcgg
     1381 cttatgaaca ggctctattg ctactacttc aatgatttat atataagaga gaagaagaga
     1441 gtggggaggg aacatggaaa ttaaagcaaa gcgaggagac cttctcgcca cgctttattg
     1501 gacgcaaagc atcgttgaac gccgcaatac catgccgata ttggcgaatg tcctcctcga
     1561 gtctcagaaa aaagacatcc gcgttaccgc taccgatttg gaagtcggtg taagaggaag
     1621 cctcgaagga gacgttgtca aagagggctc agtcacagtt aacgcgaaga agctttatga
     1681 gatcgttcgg gaagttccga acgatcacat tcaactaaag cggctggaaa atgattgggt
     1741 cgaaatccga agcggaaaat ccgtctttaa aatcgtcgga atggatgccc gcgagtttcc
     1801 gcagtttcca aaatttaatc gcgatggttt gacaaccaca cctgcatcga cgcttcggga
     1861 aatgatcgaa ggcacaatct tttctgtctc tacagacgaa acgcgttaca gtttgaatgg
     1921 agtttacgtc gagcagggtg aggccggtaa tgttcgtctg gtagccacgg acggtcaccg
     1981 gctggctttt gacgagagaa acctcggctc ttttggactg gcaaaaggtg tgattttgcc
     2041 ccggaaaggg ttagcggaac ttaagaaact tctggagagc ggcgatgatg gagttgtttc
     2101 tattggcttt cgagagaaca tggggcttgt catgaaagac agagtcgaat tgttcatgcg
     2161 gctcatcgat ggtgacttcc cggattatac gaaagtgata cccaaaggaa atcagaatat
     2221 tgctcgtatc gagcacgacg aatttttgca agctctgcgg cgggtttcga ttatttcaag
     2281 cgaacgctac aagggcatca aaatggaatt caatgaggat aagctgtcga tttcagccaa
     2341 taatccggac ctcggcgaag caaccgaaga agtagaaatg gagtacaaag gaaagccgat
     2401 cagtatagga tttaatgcgc gttatctgat tgacgtgctc catgtgctcg gcactgatgg
     2461 ggaggtgcaa atcgagctaa aagacgaact cagcccgagc ttggtgaaga agggcggcaa
     2521 agaaggctac ctttatgtcc tgatgccgat gcgtttgtga tttttttgat actctaactt
     2581 gggtgcgtga cttgaatgtg atttgttgcg aaatctagct ccggtacttt tcatctaact
     2641 ggtacttttc ttctaaaccc acaatcctgt taaattcatc gaagggcatc aagcggtcca
     2701 aaatatccac cgttgttccg tctcgacgaa gggtcgtata aacctcattt agcgactttg
     2761 ctaccgaata aagcggcgcc agcggccaaa cgaccagatc gaaccccaga tcacatagtt
     2821 cttgtggtcc catcaaagga gtgacacccc gttctagcat attggctact aatggtcccg
     2881 gcacacagcg cgcgatctcg agcatctctt ctttggtttc cggagcctca ataaacactg
     2941 catcggcgcc ggccttcttg aatgcaattc cacgggtgat cgcctcattc aaacctagag
     3001 cttgtcgcgc gtcggtgcgc gcaacgatga agaagtcgcg gttccttttg gcgtctatgg
     3061 cagcctgcaa tttttttagt tgttcttcga gtggaatgac ctgcttgcct ttcatgtgcc
     3121 cacatcgctt aggccacacc tggtcttcta aaaacatgcc ggcagcgcca attctgatca
     3181 attcatccac ggttctaata acgttgatcg cgttgccgta gccggtatcg gcatcgacga
     3241 ttaccggcaa agcggtcacc gaacaaattc gctgagctgc cgacaacatt tccgtttggg
     3301 ttagaatacc gaaatcaggc tccccgagca tcgttgcggc aaggctataa ccgcttataa
     3361 agataacttc gaaacctgtc ttagtggcga tccgtgcgct cagcgcatcg tagactccag
     3421 gcacaaccaa ctggccgctt tttttcaatg atcgccgaat catttccgct ttgtccatag
     3481 gaaaccccta agccggattt atgaacagct gatcgggcac gtgcttcggt gaacgaatcg
     3541 gtgggaagtt aactcagcgc cgcggcccgc cgcggccaaa accgcgctgc tgtcttccag
     3601 gctcgggctt aggtgcggcg cgcaatagcc tggcagattt taagcctaaa atatcagcgc
     3661 cgtagcgtga ttgatccagg tgatcatagc gagccttgat cttctgacgg atctgattgc
     3721 gcctggtcat atctcggaga gcagctttga gatcttcgag atcgagcgag acaacggcgt
     3781 catgaccacg cgctgtaaaa tgcagcgtaa atcgtgatcc ttcgccctca cgcgcgctgt
     3841 aatccatttt tttatcgatc gtacgaatga tttcgcgaag taaagcttct tcttcttgat
     3901 gaacgccttt tccaaacata atgatgtcct ccctggcaaa tagagaatgc cccctgagct
     3961 acttaatggg atgagaacga taagcaccca tctgccaatt accgccaggt tgtgaaaatc
     4021 tacgctaaac tacaggcaag atcaatcacc attagaaggt tcccgcgt
//
LOCUS       DASUTG010000308         3020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1233540, whole genome shotgun sequence.
ACCESSION   DASUTG010000308 DASUTG010000000
VERSION     DASUTG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233540"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VF089_07045"
     CDS             complement(<1..920)
                     /locus_tag="VF089_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6173755.1"
                     /translation="MDTLRFITCGSVDDGKSTLIGRLLYDTKLILADQLEALTTDSKK
                     FGTQGGRLDFALLVDGLQAEREQGITIDVAYRFFATSKRRFIVADTPGHEQYTRNMIT
                     GASTAQAAVLLIDARKGLLVQTKRHSRIVSMLGIRHVALAINKMDLVNWSRAVFDEIS
                     DSYARFAKDLGFDTITVIPVSALEGHNIVEADHVTMSWYTGPSLLQWLETVEPHDSDK
                     VKPFILPIQWVNRPHLDFRGFSGRIASGTLRPRDSVRIIPSGVVSRIKEIVVCGGAQP
                     TASVGQSVTLTLEDEIDASRGDLIAAASHPV"
     gene            complement(920..1822)
                     /gene="cysD"
                     /locus_tag="VF089_07050"
     CDS             complement(920..1822)
                     /gene="cysD"
                     /locus_tag="VF089_07050"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX6173756.1"
                     /translation="MIKESVVEELESESIEIIREVAATAAKPVMLYSIGKDSSVMLRL
                     AQKAFFPGPIPFPLMHIDTGWKFREMIAFKHRMATEVGFELITYMNPRGLAEGIGPVS
                     HGSAYHTDVMKTEALKQALDFYGFDVAFGGARRDEEKSRAKERIFSFRAPGHRWEPKA
                     QRPELWNLYNTRINPGETIRVFPLSNWTEADIWAYIRQENISIVPLYFAANRPVVKRC
                     GQWIMLDDNRLPLEPGEKPDIRMVRFRTLGCYPLTAAIESTATTLDEIVTETLAATTS
                     ERQGRIIDHDGAGSMERKKQEGYF"
     gene            complement(2226..>3020)
                     /locus_tag="VF089_07055"
     CDS             complement(2226..>3020)
                     /locus_tag="VF089_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX6173757.1"
                     /translation="VVLSILIGGPFLYVVLWLMQAGEYWWLWAFLFITIFQLLMIIIF
                     PTFIAPWFNKFEPLASGELRDRILALANQIGFKASGIFLMDGSKRSAHSNAYFTGIGK
                     AKRIVLFDTLVEQMTIDQGVAVLAHEMGHYKMKHMRRMMIVQGVFLFFGLYILSRVVA
                     YEPLFTAFGLQPSNHAALVLFSLLSGPFTFYLGPLMNLLSRKHEYEADRFAVITLGSG
                     KPMEEALINLTVRNLSNLTPHPWYSAYHYSHPTPTERIGAIRQITT"
BASE COUNT          752 a          789 c          787 g          692 t
ORIGIN      
        1 acgggatgtg aagcggccgc gatgagatcc ccacggctag catcaatctc atcctcaagc
       61 gtcagagtca ccgactgacc tacgctcgct gtaggttgag ctccgccaca gacgacgatt
      121 tccttgattc tgctaaccac acctgaggga atgatccgca cgctatctct gggcctcaaa
      181 gtgccactcg cgatacgccc cgaaaagccc cggaagtcta gatgaggccg attaacccac
      241 tggatcggca gaatgaaggg cttaaccttg tcactgtcgt gtggctcaac tgtttcgagc
      301 cactgcaata ggctcggtcc tgtataccag gacatcgtaa cgtggtccgc ttcaacgatg
      361 ttatgacctt caagagccga caccgggatc acggtaatcg tgtcgaagcc gagatcctta
      421 gcgaaccggg cataggaatc cgagatttcg tcaaacaccg ctcgtgacca gttcacgagg
      481 tccatcttgt tgatagcaag agctacatgg cggatgccaa gcatcgagac aatgcggctg
      541 tggcgtttgg tctggactag caggcctttg cgagcgtcaa tcagcagcac agccgcctgc
      601 gcggtggacg cgccagtgat catgttacgg gtgtactgtt catgccccgg agtgtcggcg
      661 acgataaagc ggcgcttgct ggttgcaaaa aagcggtaag cgacatcgat agtgatgccc
      721 tgttcacgtt cggcctgcaa gccgtcgacc agcagtgcaa agtccaaacg cccgccttgg
      781 gtgccgaatt ttttggaatc ggttgtcagt gcttccaact gatctgcaag tatcaactta
      841 gtgtcgtaaa gtaagcgccc aatgagtgtc gacttgccat cgtctacgct gccgcaggtg
      901 atgaatcgca aagtgtccat caaaagtaac cttcctgctt tttgcgttcc atggagccgg
      961 cgccgtcatg gtcgataata cgtccctgtc gctcggaagt ggtggcggcg agagtttcgg
     1021 tgacgatctc atctagcgtc gttgcggtac tttcaatcgc ggcggtcaga ggataacacc
     1081 caagcgttcg gaagcgcacc atacgaatat ccggcttttc gccgggctcg agcggcaatc
     1141 ggttatcgtc gagcatgatc cattggccac aacgcttgac aacagggcga ttggcggcga
     1201 agtacaaggg aacgatcgaa atgttctcct ggcgaatgta ggcccaaatg tcggcttcgg
     1261 tccaattgga gagtggaaat actcttatgg tctcgcccgg attgattcga gtgttgtaga
     1321 gattccagag ttctggccgc tgcgcttttg gctcccaacg atggcccggc gctcgaaacg
     1381 aaaagattcg ctccttggcc ctcgacttct cctcgtctcg tcttgctcca ccgaaagcaa
     1441 catcaaaacc gtagaaatca agcgcttgct tgagcgcttc tgtcttcatg acatcggtgt
     1501 ggtacgccga accatgggag actggtccga taccttcggc aagccctctc gggttcatgt
     1561 aggtaatcag ctcaaaccct acttcggtgg ccatgcgatg tttaaacgca atcatttcgc
     1621 ggaacttcca tccggtgtcg atatgcatca atggaaatgg aatcgggcca gggaaaaaag
     1681 ccttctgcgc caagcgcagc atcactgatg agtctttgcc aatcgagtac agcatcaccg
     1741 gtttcgccgc tgtagctgct acttcgcgga ttatttcgat cgactcagat tcaagctcct
     1801 ctaccacgct ctctttaatc attgaacatc acccgtagtt gaagtagctg cgcagtaaag
     1861 attctgcgat tcggtaaata cggtggaggc acctgcagtc atcatccagg atttcgaaca
     1921 tgagagcggc ctcagattgt tcatgactca cttatcgggc gtctcccata gctgtgtcac
     1981 gcacgaaaga agagtatcgt tcgtagatcg ccagaaaacg caacagagta agacatcgag
     2041 cagcggcggc agctaacgtt cagcaagtaa ctgtacgggc gctaacggta cacgaaagta
     2101 actctacgag tcaaatatta ttgcatgtta gcgctcccgg aaaggatgcc gcggcaaatt
     2161 ggaatgccag aaggctcctg agaagaccag cgccgtctag gctgcttcat tcggtgtgaa
     2221 gtttgctagg ttgtgatttg acgaatcgca ccaattcgct ctgttggcgt agggtgcgag
     2281 tagtgatagg ccgaatacca cggatgcggc gtcaggttcg acaaattcct tactgttaaa
     2341 ttgattagtg cttcttccat gggcttgccg ctgcccaacg tgattaccgc aaaacgatcc
     2401 gcctcatact cgtgcttgcg cgacaaaagg ttcatcaatg ggccgaggta aaaagtaaat
     2461 ggtcctgaaa gcagactgaa gagaactaac gctgcgtgat ttgatggctg taagccaaaa
     2521 gcagtaaaca gcggttcata agctacaacc cggctgagaa tatataaacc aaaaaataga
     2581 aacacgccct gcacgatcat cattcggcgc atgtgtttca ttttgtaatg tcccatctca
     2641 tgcgccaaaa ccgcgacgcc ctgatctatt gtcatctgtt caaccagggt atcgaaaagc
     2701 acaattcgtt tggccttgcc aatccctgtg aagtaggcgt tggaatgagc tgaacgcttc
     2761 gagccgtcca tcaaaaagat gccgctggcc ttaaaaccga tctgattcgc aagcgccaat
     2821 atccgatcgc gcagttctcc actggccaat ggttcaaact tgttaaacca gggggcgatg
     2881 aacgttggga aaattatgat catcaggagt tggaagatgg tgatgaaaag aaacgcccag
     2941 agccaccagt attcgccggc ttgcatcagc cagagcacga catatagaaa aggaccgcca
     3001 atcagaatgc tcagcactac
//
LOCUS       DASUTG010000309         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_789257, whole genome shotgun sequence.
ACCESSION   DASUTG010000309 DASUTG010000000
VERSION     DASUTG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789257"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1067)
                     /locus_tag="VF089_07060"
     CDS             complement(234..1067)
                     /locus_tag="VF089_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6173758.1"
                     /translation="MPTANLNDVQIYYEDSGEGEVVLLAPPSWWPCDTWKINVVPFLS
                     RRYRTIIFDCRGTGYSSKPDHGYTISQFAKDCAGLLEQLKIIRAHVAGFALGGQIAQA
                     LAIERPDLIATLTIAAAGAGMKATDGAPRQMRNTDEEEIREHGFERFIRGHVENTHMA
                     FGERFYREHPEVVRALSDALWKRQTSPEQHRYHYEARRTWDTLGNAPKVKVPTLILCG
                     AEDDVNRGGSTPVATARRLAELVPGSELALVPNVKHMTFWDGAGTLIALENFLQRHPI
                     H"
     gene            complement(1341..1721)
                     /locus_tag="VF089_07065"
     CDS             complement(1341..1721)
                     /locus_tag="VF089_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173759.1"
                     /translation="MARHGFSEAAWEAAKADGKAALVECARAKKMISYTDFMHQIRSI
                     SFDTPHDSRLPQFLAEISTEEAKAGRGMMTALIVRKNGDQRPGAGFFQLAKRLGYEVS
                     DPEKFWLEEVDKVFASWQNEASRS"
     gene            2372..3400
                     /locus_tag="VF089_07070"
     CDS             2372..3400
                     /locus_tag="VF089_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173760.1"
                     /translation="MKTFLVALLLLSMSSTSVGAQADFYKGKTIRVVIGYSAGGTNDL
                     WARAIARYWGKHIPGNPNFIVQNMPGAATMVAANYIYGVEKPDGLTLGLISPALFFNQ
                     LGGKKEAKFDWAKFTWIGSPEETSEMLSMRSDTPFKTLDDIRQSSVPPKCGTTGIGSP
                     GHYFPRLLEEVLELKFDLVTGYPGASDIDLAIEKGEVQCRAGTTSAFFGRDPGRTWAK
                     TGFVRILVIGGSKRDQRAPNAPTIWEAMEKFKTPDAGKRLATVLLSPGSFGRPIVGGP
                     GIPADRTKMLQEGFMKAMKDPELLAEAEKRRWEPDPVSGEKLQALAGEVVGQPPEVIK
                     RMNAVLAN"
     gene            complement(3520..4578)
                     /locus_tag="VF089_07075"
     CDS             complement(3520..4578)
                     /locus_tag="VF089_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173761.1"
                     /translation="MLKKQLIESFWATVVFVIAVLADSALAQAPFYQGKTLVVVAGTA
                     PGGAGDHRTRALIPFLRKYIPGNPSIVIEHKAGGGGRQAANHIYQVAKPDGLTVGALS
                     GSIVALNLLGAAGVMYDIDKFIYLGASESTSHQIMYTRKELGLSSLEKLRAASGIRIG
                     AQAVGHPNYIAGRIFAYFLGLKEPRFIVGYTTPEIEVALLRAELDGRANNVAQLLHRN
                     PDWVEKSVMDIHAILEIPKGSKHSRFGLVPEIESFAKSENETKLLAMYRTFRLVGAPY
                     TLPPGTPKDKVKILQDAMRRAFKDPDFHREYKKLTGEEPDPLLPEEMEKAIMEMPRDP
                     EVIDLLKRLSAEGTLPVR"
BASE COUNT         1147 a         1486 c         1396 g         1211 t
ORIGIN      
        1 aaacgatggc atcggttggc ccccagatga ctactcgccc ttccttcctg gcggcgtggt
       61 aaagttccct gatgccgtcg ctgtcaccct tgcctttttc ttgggcgcag cccgtatcgc
      121 taaagaaaac gctcatcaaa cagatcatga ctgtcgcgac gaatgctcta gatttcatag
      181 gccaatacct cccgatcttt tgcgcccaaa attcaatcct caacgttcaa tggctagtga
      241 atcggatggc gctgcaaaaa attttctaac gcgatcagcg tgccggcgcc gtcccaaaaa
      301 gtcatatgct tgacgttggg tacgagcgcc agctcgcttc cgggaacaag ctccgcaagt
      361 cgccgtgccg ttgccaccgg agtgctaccg ccgcgattaa cgtcatcctc cgctccgcaa
      421 agaatcaagg ttggcacttt tactttcggc gcgttgccga gggtgtccca agtgcgcctc
      481 gcctcgtaat gatagcgatg ctgctccggg ctcgtctgtc tcttccacag ggcatccgac
      541 agcgcgcgga cgacttccgg atgctctcgg tagaatcttt caccgaacgc catgtgcgtg
      601 ttctcgacgt gaccgcggat gaagcgctcg aagccgtgct cgcgaatctc ttcttcatcg
      661 gtgttgcgca tctggcgggg agcgccgtcg gttgccttca ttccggcgcc tgccgccgcg
      721 atcgtcaagg ttgcgatcaa gtcgggccgt tcgatggcga gagcctgagc aatctgaccg
      781 ccgagcgcaa atcccgccac atgagcccgg atgattttca gctgctcaag aagcccggcg
      841 cagtctttag cgaactggct gatcgtgtag ccatgatccg gtttgctgct atagccggtg
      901 ccgcgacaat cgaaaatgat cgtgcggtag cgccgcgaca gaaacggcac gacattgatt
      961 ttccaagtat cgcatggcca ccaggaaggc ggcgccagca aaacgacctc gccttctcca
     1021 gaatcctcgt aataaatctg cacatcgttt aaattggccg tcggcatttt tttatctcca
     1081 agggattagt caggcctttt gtacagcgag gacagacttt caagttcgtg atgaaattgt
     1141 tcggtcgaga cctaatccaa aatcgatttc gctgcaacag gtcacgtggg tgacatgaag
     1201 cacacgggga ctgatgggga tttaaactgc actgctaagt ggcaaaccga acgcgattcg
     1261 atcgcagggg gcagtaaagc tgatgagcca tctctaagac cactgaaggt ctcaaagaac
     1321 caagagccgg cttattcgcc tcatgaacga ctggcttcgt tttgccagct agcgaacact
     1381 ttgtctactt cttcgagcca aaacttctcg ggatcggata cttcgtaacc aaggcgtttg
     1441 gcgagctgga aaaatccggc ccctggcctt tggtcgccgt tctttctaac aatcagtgcg
     1501 gtcatcatgc cacgccctgc tttggcttct tccgtggaga tttctgcgag gaattgcggc
     1561 aatcgcgaat cgtgaggagt atcaaatgag atcgagcgga tctgatgcat gaagtctgtg
     1621 tatgaaatca ttttttttgc acgggcgcac tcgaccaaag cggcttttcc gtcggctttt
     1681 gcggcttccc atgcagcttc gctaaatccg tgtctcgcca tattccccgt caatcttata
     1741 tccgagcgtc aggttcgccg tacgcaaagt tatattcacg acccatcttt ggaacgatcc
     1801 tcgagctgcg aggattttta ttttttgttc gaagccaaaa caactcggaa ggctccgaaa
     1861 aatctcctgt ccgcggacca gtcggcggta gaccgagaat gactatgaaa tagccgaaag
     1921 ctttttcgat cgccacggcc gcgggacttt ccactcttag cctggcaatt tcgtcggtga
     1981 aaggacagcg ccttgagaca tcctcgcctg ccggttgcct ttcgttgagc ggcggagagt
     2041 tgctcggtct ttgtgatgct ccaaccaacg cgcccgtcaa accacgggag atcgaacagc
     2101 cgccgtcttg gtaaattcga ccacaaacgt tctcgcgctg tgctcgaaac gcgatcaccg
     2161 ggttctgtag catataggca aaaccattat gaaatgtttt agacgggaga tagcctgtcc
     2221 gttttccatg gtgttcctcc tcgcctcgtg gcgcctcact tcttttcggt agtcttgact
     2281 tagcgcctgc atcaactata tgtgcctcag cgtgaggccg agtgggcagg acggagccga
     2341 caactcttac cgaacgaaag gagtgcgggg cgtgaaaaca tttcttgtcg ctcttttgct
     2401 actgtcgatg agcagcacga gtgtcggcgc tcaagccgat ttctataaag gcaagacgat
     2461 ccgagtcgtg atcgggtatt ccgccggagg caccaatgac ctctgggcgc gagcgatcgc
     2521 gcgatattgg ggcaagcaca ttcccggcaa tcccaatttc atcgtgcaga atatgccggg
     2581 tgccgccacg atggtcgcgg ccaactacat ctatggggtc gagaagccgg acgggctcac
     2641 cttgggactg atctctcccg cgctattttt caaccagctc ggcggtaaaa aggaagcgaa
     2701 gttcgactgg gcgaagttta cctggatcgg ctcgcccgag gaaaccagcg agatgctgtc
     2761 catgcgctcc gacacgcctt tcaaaactct ggacgacatc cggcaatcct cagtgccgcc
     2821 caagtgcggc accaccggca tcggctcacc gggccactac tttccgcgcc tcctcgaaga
     2881 ggtcctcgag ctcaagttcg acctggtaac cggttatccc ggagcgtcgg acatcgacct
     2941 tgctattgag aagggagaag tccaatgccg cgccggaaca accagcgcgt tcttcggcag
     3001 ggatccgggc cgcacgtggg cgaaaaccgg cttcgtgcgc atcctcgtga tcggcggctc
     3061 gaagcgagac cagagggcgc cgaacgcgcc gacgatctgg gaagcgatgg aaaaatttaa
     3121 aacaccggac gccggcaaac gcctcgccac ggtgcttctc tcgcctggtt cattcggccg
     3181 cccgatcgtc ggcggccccg ggattcccgc cgatcggacc aagatgctac aggagggttt
     3241 tatgaaggcg atgaaagatc ccgagctctt ggccgaggcg gaaaagcgcc gatgggaacc
     3301 ggatcctgta agcggcgaaa aacttcaggc gctcgcgggc gaggtagtgg gccagccgcc
     3361 cgaggtcatc aagcggatga acgcagtgct ggcaaattaa gctcggcagg tttaatcaca
     3421 ggaccgttca ggtgtcactg aacggtacag aacagtttgg gcggctcgaa aggaggccgg
     3481 ctcactgctg tcacacaatg acgctccata cgctgccagt tatcgcacag gaagcgtgcc
     3541 ttcagcagaa agtcttttta acagatcaat cacctccggg tcacgcggca tctccatgat
     3601 cgctttttcc atttcttccg gcaggagcgg atcgggttct tcaccggtaa gctttttata
     3661 ttcgcggtga aaatcggggt ctttgaacgc cctgcgcatt gcgtcctgaa gaattttgac
     3721 cttgtccttc ggggttcccg gaggcaaagt gtaaggcgcg cccacaagtc tgaaggtccg
     3781 gtacatagcg aggagcttgg tctcgttttc agactttgca aaactctcga tttcggggac
     3841 aagaccgaag cgagagtgct tagatccttt cggaatctct aagatagcgt ggatatccat
     3901 cacgcttttt tccacccaat ccgggttccg gtgcaatagc tgtgcgacat tattcgctct
     3961 cccatcgagc tccgcgcgta atagagctac ctcgatttcc ggagttgtgt agcccacgat
     4021 aaacctcggc tccttcaaac ctagaaaata ggcaaaaatc cgcccagcaa tatagttagg
     4081 atgaccgacc gcctgggccc cgatcctgat accggaagcc gctcgtagct tttcgagact
     4141 gctcagacca agttcctttc ttgtatacat gatctgatgt gaagtgcttt ccgacgcacc
     4201 gaggtagata aatttatcga tatcatacat gacaccggcc gctccgagga ggttgagcgc
     4261 gactatgctg ccgctcaagg ctccgacggt cagcccgtcc ggcttcgcga cctggtatat
     4321 atgattggct gcttgtcgac ctccaccgcc agctttgtgc tcgatcacga tcgacggatt
     4381 tccggggatg tatttccgca gaaatggaat gagggccctc gtgcggtgat ctcccgctcc
     4441 tccgggagcg gtgcccgcga caaccactaa ggtcttaccc tgataaaagg gcgcctgcgc
     4501 caaagcgctg tcagccagaa ctgctataac aaaaactacc gtggcccaaa atgattcgat
     4561 caattgtttc tttagcatgg cgtacctccc cgtcagcgcg ggcggaacag gtcccgcgcc
     4621 gacaagcctt tgaagaactc aatgacctcg accgaatgtt tctcgtgcgc gtaccccccc
     4681 tataggtata gtcaagccgt acgcaacgat agcgacgatc atcgatagcc actgcatgag
     4741 ctgacttaag gcgagttggt gagcaggttc gaccataagg tgaccggatt gggcatcgaa
     4801 tcaaagcaaa aatttcaccc gctgatgttc acagcttccc ttgaggaaaa gaacgctcgt
     4861 aatcttctgg cttgtgaaat actgtgccgc gaccatgcga cgagatccga atggcatggc
     4921 cggcctgttt tcctctggga atgttggcac aaaacgcttc tagcataccc ccgtcgcggc
     4981 cttttcatgg cggagaagta gcctggtttt ttgggatcgt atagtcgcca cctgcatttc
     5041 gtcctctctt ccggacttta cccttttccg cctgactgca acgacctccc gattatttac
     5101 gaaactccag cttcaagaac tagcgggcga gcgcaacttt ttttcttgcc tcggctaagt
     5161 agccgctgtc ttcgaggatc ttcacgacgc ttaggttgat cgcttcgttg acatcgacct
     5221 ggacaatctt ggggttgacc
//
LOCUS       DASUTG010000310         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1828117, whole genome shotgun sequence.
ACCESSION   DASUTG010000310 DASUTG010000000
VERSION     DASUTG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828117"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..>2599)
                     /locus_tag="VF089_07080"
     CDS             complement(268..>2599)
                     /locus_tag="VF089_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6173762.1"
                     /translation="PFTPSIADLQKAKIDQPSMLISSDDKRIATYKPMNREWVGLSRV
                     SPHVINALIATEDHRFYQHYGVDVRRTLSGVFRTVLGDIEGGSTLTQQLARNLYPEEI
                     GRERTITRKIKETITAIKIEYAYTKKEILETYLHSMPFLYNAFGIEMAARTYFDKSAQ
                     KLDVIESATLIAMLKGTSYYNPVLNPERALRRRNVVLSQMVKRGVLSQTEFDRLKTRP
                     IRLDFERQPEAIGIAPHLAAHIRKWLIDWADRNEHNIYADGLRVHTTIDSRLQTIANQ
                     AVNRQTESLQAVADVEWALSSPRLISSNPKAYVDMRRRVQPFAYFWKSKSALLDVFIR
                     ESGAYRAALQSGMAPETALALLKDDSEFMSKLRAAKTRLQAGFVAIDPATGEVKAWVG
                     SREFKTDQYDHVAQARRQPGSTFKAFVYGAALEQGMSPNKRFTDSPVEIRLPDGTIWR
                     PSDLGQPTGKRMTAREGLIYSKNTVTAQVMQEIGPKKTAELARKMGINQSKLDEVPAL
                     ALGTSPVTLLEMVSAYSTIAASGVYRQPIFITRITDNKGKVLAEFATKDKGGMSEKPV
                     GDLINMLRGAVDQGTGQAVRTQFGLRGDFAGKTGTTQNNSDGWFILMHPRLVAGSWVG
                     FNDPRVTMRSNYWGEGGHNALLLVGDFFQQTLASRLIDSRADFPFDRYYESLWDPYFD
                     MAREWFGGMLKDWFGGERAKPEPLPPASPRRDLRFEAPGSEQSPGEKERILEQFGGTQ
                     DRREQRQLEREAEKDRLLERLRQKQQQREQENRARD"
BASE COUNT          520 a          754 c          704 g          621 t
ORIGIN      
        1 taatggaccc atgcatgaaa acgcggcgta ttaaatacca ccttgcgccg cttttgttca
       61 tacgccaact ggagcaaatc gataacctct accgccatag taatctcctt cctcagtact
      121 gatacaatgt gacaagttaa aatagcaatg cgcgtctgga tcgccggctc tcgtccgccg
      181 gaaggtcgat catcaacatc aggggctcac agccgataag cagagcggga ggtatttgac
      241 cggtcactat tcgtacagat ctccgtctca gtctctggcg cggttctcct gctcgcgttg
      301 ctgttgcttc tgcctcaatc gctccaaaag ccgatccttc tccgcttccc tttcgagctg
      361 ccgctgctcc cggcggtctt gcgtaccccc gaattgctcc aatattcgct ccttctcgcc
      421 cggcgattgt tcagagcccg gcgcttcgaa tcgtaagtcg cggcgcggcg aagccggcgg
      481 taacggctcc ggcttggctc tctctccacc gaaccaatct ttgagcatgc caccgaacca
      541 ttccctggcc atgtcgaagt agggatccca caacgactcg tagtatcggt cgaatggaaa
      601 atcggctcgg ctgtcgatta gccgggacgc aagcgtttgc tggaaaaaat caccgaccag
      661 cagtagcgca ttgtgtccgc cctcgcccca ataattgctg cgcatggtga cccgtgggtc
      721 gttgaagccg acccatgagc cggcgaccaa ccgcgggtgc atcaagataa accagccatc
      781 gctattattt tgcgtggtgc cggttttgcc ggcgaaatcg ccgcgcaaac cgaactgcgt
      841 gcgcaccgct tgcccggtgc cttgatcgac tgcgccgcgc agcatgttga tcagatctcc
      901 gacgggcttc tcgctcatac cgcccttgtc cttggtggcg aactcggcga gcacctttcc
      961 cttgttgtca gtaatgcggg tgataaaaat cggctgccgg tagacgccgc tggcggcgat
     1021 cgtgctgtat gcagaaacca tttccaatag agtgaccggg ctggttccga gcgctaatgc
     1081 cggcacctcg tcgagcttgc tttgattgat tcccatcttg cgcgcaagct ctgcggtctt
     1141 tttcgggccg atttcctgca tgacctgagc ggtgacggta tttttggaat aaataagacc
     1201 ctcccgcgcc gtcattcgct tgcccgtcgg ctgcccgaga tcggatgggc gccatatagt
     1261 tccatcgggc aggcgaattt ccactgggct gtcggtaaac cgcttgttcg gcgacattcc
     1321 ctgctccagt gccgcgccgt aaacaaacgc tttaaaggta gagcccggtt gtcgtcgggc
     1381 ttgcgccaca tgatcgtact gatcggtctt gaactcgcgg ctaccgaccc acgcctttac
     1441 ttcgcccgta gccggatcga tcgccacaaa gccagcctgc aaccgcgttt tggcggcgcg
     1501 tagcttgctc atgaattcac tatcatcctt gagcagcgcc agcgccgtct caggcgccat
     1561 gccggattga agcgcagcgc gatacgcccc ggactcccgg ataaacacgt cgagcaaggc
     1621 gcttttgctt ttccaaaagt aagcgaaagg ttgcactcgc cgccgcatat caacgtaggc
     1681 tttggggttt gacgagatca ggcgcggcga gctcaatgcc cattctacgt ctgccaccgc
     1741 ctggagggac tcagtctggc ggttgaccgc ctgattggcg atggtctgca accgcgagtc
     1801 aatggttgtg tgcaccctca gcccatcggc atagatatta tgctcattgc ggtcggccca
     1861 atcgatcagc catttacgga tgtgcgcagc aaggtgagga gcgatgccga ttgcttcggg
     1921 ctggcgttca aaatcgagac gtatcggcct ggtttttaac cggtcaaact ctgtctggct
     1981 caacacacca cgcttgacca tttgcgacag cacgacgttg cgccgacgaa gtgcgcgctc
     2041 ggggttaagc accggattgt aatagctggt gcccttcaac atcgcgatca aagtcgcgct
     2101 ttcaatcaca tcgagcttct gcgccgattt atcgaagtag gtccgggcag ccatttcgat
     2161 accgaaagcg ttgtacaaga acggcatact gtgtagataa gtctccaaaa tctccttttt
     2221 ggtataggcg tattcgattt tgatcgcggt gatggtttct ttgatttttc tcgtgatggt
     2281 ccgctcacgg ccaatctcct ccggatagag attgcgtgcg agttgctggg tcagtgtgga
     2341 gccgccttct atatcgccga gcactgtgcg gaaaacacct gatagagtgc ggcgcacgtc
     2401 taccccgtaa tgttgataaa agcggtgatc ttcggtcgcg atgagggcgt tgatgacatg
     2461 cggcgaaacg cgactcaaac cgacccactc gcggttcatc ggtttatacg ttgcgatgcg
     2521 cttgtcatca gatgaaatca gcatgctcgg ttgatcgatc ttggcttttt gtagatcggc
     2581 aatactgggc gtgaacggt
//
LOCUS       DASUTG010000311         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1383391, whole genome shotgun sequence.
ACCESSION   DASUTG010000311 DASUTG010000000
VERSION     DASUTG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383391"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..316
                     /gene="rpsP"
                     /locus_tag="VF089_07085"
     CDS             71..316
                     /gene="rpsP"
                     /locus_tag="VF089_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6173763.1"
                     /translation="MSVRIRLSRHGAKKKPFYRIVVSDQRFPRDGRYIEQIGTYDPTA
                     GGGTFKINHEKAETWIKRGAQPTRTVSQLIAKQKKAS"
     gene            341..577
                     /locus_tag="VF089_07090"
     CDS             341..577
                     /locus_tag="VF089_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6173764.1"
                     /translation="MKELVQYLARSLVSNPDAVEVAESALADGAVYELRVAKDDLGRI
                     IGKQGRTAKSIRMILNAAASKTNRKVSLEIVEEK"
     gene            599..1111
                     /gene="rimM"
                     /locus_tag="VF089_07095"
     CDS             599..1111
                     /gene="rimM"
                     /locus_tag="VF089_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6173765.1"
                     /translation="MPDGIVPLGKIVTTHGIAGWLKLNPYNSGSPLLSNLRDVILVRD
                     ETRSAVQVQSARPHRQQILLKLCGIDDIEGARHWVGSTLAVREEALAPPARGQYYHYQ
                     VIGLEVFDTSGARIGTLKEIWSTPASELYVVAGLDKEHLIPAVKEMIAEVDLAAGRMI
                     VDLPAGLLDL"
     gene            1138..2412
                     /gene="trmD"
                     /locus_tag="VF089_07100"
     CDS             1138..2412
                     /gene="trmD"
                     /locus_tag="VF089_07100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6173766.1"
                     /translation="MLTFTIVTLFPAMFESPLNHSILKKAQEKGLIAVGLIDPRDYTT
                     DRHRVTDDYPYGGGQGMVMKPEPLVAAIEDVKTKMPRSKVILLSPQGRVFNQALAAEL
                     AKEEELVLLCGRYEGVDERVKDFIDDEISIGDYTISGGEPAATVVVDAVSRLIPGVLG
                     NAKSAGDESFSNGLLEYPQYTRPEEFRGAKVPEALLSGDHQRIRQWRRQMSIQQTWKR
                     RPDLIGRVDLAPEERELICNQRAPVHVALLHYPVYDKNRQIVTTAVTNMDIHDIARSG
                     RTYGIKGFYVVIPVKALQRLALKIIDHWEHGYGSQYNATRKEALALVRVANTLDDVLI
                     DLEQEYGVKPRIVVTSARSGGHRTSFAQLKDMLINTTHPFLIVLGTGWGLTESIISQS
                     DYVLEAIEGWTDYNHLSVRSAAAIVLDRLLGR"
     gene            2436..2792
                     /gene="rplS"
                     /locus_tag="VF089_07105"
     CDS             2436..2792
                     /gene="rplS"
                     /locus_tag="VF089_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX6173767.1"
                     /translation="MNILDKLETQQSKQEVPALRAGETVRVHVKVVEGEKERTQVFEG
                     IVIGIGGKGNRANFVVRKISYGVGVERIFPFHSPRIDKVEVVSRGRVRRAKLYYLRER
                     SGKAARLFSDERSGSR"
     gene            complement(2800..>2985)
                     /locus_tag="VF089_07110"
     CDS             complement(2800..>2985)
                     /locus_tag="VF089_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6173768.1"
                     /translation="VEARIAARNLQLEGKLIGSVQVIGCFEIMPGGHFKGDAQAAELR
                     VHSGATFDGQGKITGAL"
BASE COUNT          761 a          753 c          812 g          659 t
ORIGIN      
        1 agcaagggcg gaatgaaatc gctctttggc cgaatggcca acccgtttca ctagaggaga
       61 aggaaatcgt atgagtgtaa gaattcgtct gagcagacat ggcgcaaaga agaaaccctt
      121 ttaccggatc gttgtcagcg accaacgttt tcctcgtgat ggcaggtaca tcgagcagat
      181 cggcacctat gatccaaccg cgggcggagg aacattcaag atcaatcacg aaaaagccga
      241 gacttggatc aagcgcggcg ctcaaccgac ccgaaccgtt tcccaactga tcgcgaaaca
      301 aaagaaagcc agttagtaat ttagccaagt ggcgcgacat atgaaggaac tcgttcagta
      361 cttggccaga tccctggtga gtaacccgga tgccgttgaa gtagcggaga gcgcattagc
      421 ggacggcgct gtttatgaat taagagttgc caaagatgat ttggggcgga ttatcggcaa
      481 gcagggccgg actgcgaaat caatacgcat gatcctaaat gcagcagctt caaagaccaa
      541 ccgtaaagtc agtttggaaa tcgtagaaga aaaatagaca gtctgttagg ctatttcagt
      601 gcctgacgga atcgtcccat tagggaaaat cgttacgaca catggaattg ccgggtggct
      661 taagctgaat ccctacaatt ccgggagccc tcttctatca aatctacgcg acgtcattct
      721 agttagggat gaaacccgga gcgccgttca ggtgcaatcg gcccgtccgc acaggcaaca
      781 aatcttgctg aagctgtgcg ggatcgatga tatcgaaggc gcgcgccatt gggtgggatc
      841 gaccctcgct gtgcgcgaag aagcactggc gcctccggcg cggggccaat actaccatta
      901 ccaagtgatt ggtctcgaag tcttcgatac cagcggcgca cgaattggaa ctctaaagga
      961 aatctggtcg acgcccgcta gcgagctgta tgtcgttgcc gggctcgata aagaacatct
     1021 aattccggct gtgaaagaaa tgatcgccga agtggatttg gcagctggcc ggatgatagt
     1081 cgatcttcct gccggtctcc tcgatctctg aaccgaacct cgtctgcccc ccaacgcatg
     1141 ctcactttca ctatcgtcac tctttttccg gcgatgttcg aatcaccttt gaaccacagc
     1201 attctgaaaa aggcccagga gaaaggtctg attgcagtag gccttatcga tccacgtgac
     1261 tacacaactg accgccatcg cgtgaccgat gattatccct acggcggcgg tcaaggcatg
     1321 gtgatgaaac cggagccgtt ggtggcggcg atcgaggacg ttaaaacaaa aatgccgcga
     1381 tccaaggtca ttcttctatc gccgcagggt cgtgtcttca atcaagcctt agcagctgag
     1441 ctggccaagg aagaagaact cgtgctgctt tgcggccgct acgaaggagt cgatgagcga
     1501 gtcaaagatt ttatcgacga cgagatttcg ataggcgact acacgattag cggcggcgag
     1561 ccggcggcta cagttgtcgt cgacgcagta tcccgtttga tcccgggcgt attgggcaac
     1621 gccaaatctg ccggggatga gtcttttagc aatgggctgc tcgagtatcc tcaatatacc
     1681 cggccagagg aatttcgcgg cgcgaaggtg ccggaagcgc tattatcagg cgatcatcaa
     1741 agaatacggc aatggcggcg tcagatgagc atccagcaaa cgtggaaacg ccgaccggat
     1801 ttaatcggca gagttgattt agcaccggaa gaaagggagc tgatctgcaa ccagcgggct
     1861 cctgtgcacg tggccctgct tcattacccg gtttacgata agaaccggca gatcgttaca
     1921 acagcggtta ccaacatgga catccatgat atcgctcgtt cgggacgaac atacggcatc
     1981 aaaggttttt atgtggtcat tccggtcaaa gcgctgcaac gacttgcgtt aaagatcatc
     2041 gatcattggg aacacggcta cggtagtcaa tataacgcaa cccggaaaga ggcgctcgcg
     2101 ctggtccgag ttgcaaacac gctcgacgac gtgctcatcg atttggagca ggaatacggc
     2161 gtaaagcccc gaattgtcgt gacgtccgca aggtctggag gtcaccggac ctctttcgcc
     2221 cagctcaaag acatgttaat aaataccact caccctttcc taatcgtttt gggcacgggc
     2281 tggggactca ctgagtcaat aatctctcaa tcagattacg ttttggaagc gatcgagggt
     2341 tggacggatt acaaccatct ctcggttcga tcggcagcag cgattgtttt agaccgtctt
     2401 ctgggacgct agctttattg tttataggag cagccatgaa tattctagac aaactcgaaa
     2461 cccagcaatc caagcaggaa gttccggcgc tcagagccgg agaaaccgtg cgggtgcatg
     2521 ttaaggttgt cgaaggtgaa aaggaacgaa ctcaggtctt tgagggaatc gtcattggca
     2581 ttggcgggaa aggaaaccgc gcaaatttcg tcgttcgcaa gatatcttat ggagtgggcg
     2641 tggaacgtat ctttcctttt cattccccac ggattgataa agtcgaagtc gtttcgcgcg
     2701 gcagagtgcg gcgtgcaaag ctttattatt tgcgggaaag atcaggaaag gcggcgcggc
     2761 tcttttccga cgaacgatcc ggcagccgtt aacaccggct tagagcgcgc cggttatttt
     2821 tccttgacca tcgaaagtcg ccccggagtg aactctgagc tctgcggctt gagcgtcacc
     2881 cttgaaatgt cctccgggca ttatttcgaa gcaaccaatc acctgaacac taccgatcaa
     2941 cttcccctcc agttgtagat tgcgcgcggc aatccgcgct tctac
//
LOCUS       DASUTG010000312         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_345107, whole genome shotgun sequence.
ACCESSION   DASUTG010000312 DASUTG010000000
VERSION     DASUTG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345107"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..891
                     /locus_tag="VF089_07115"
     CDS             100..891
                     /locus_tag="VF089_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX6173769.1"
                     /translation="MRKISITRKECSVTIVIPTYREVENLRPLVIRISDAMSKAKESS
                     EIIFVDDDSRDGTDRIVAELNEFGYPVRLITRIGERGLSSAVIRGFSEATGEAFVCLD
                     ADLSHPPEAIPAILQCLGETGVDFVLGSRYVPGGSTDERWGLLRWLNSKIATALARPL
                     TSAEDPMSGFFAIRRTVYERAAPLNPVGYKIALELIVKCKCSIIREVPIRFAQRQFGE
                     SKLSLGERLKYLWHLSRLLRFKHRYRMLSKAQFSRDLKKPQRPET"
     gene            911..1633
                     /locus_tag="VF089_07120"
     CDS             911..1633
                     /locus_tag="VF089_07120"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173770.1"
                     /translation="MPYDCWRGMVGMIKPTKGSGSLVELIKMLPDGIGVIPLFNNVRH
                     GKIEEFQSAIPAYEEKIAELAGDRVDLIHPAGTPPFMLLGYKGEAEIIQRWEQQFGIP
                     IFTSGTNQVAAMKALKIRKFVGIGYDFDDTRIVAKYFTEAGFEVLELGKLPGPWEEVG
                     RLSSKDIYYQAKRLFLRHPNADGIYFQGGKLRILDIVEELEQDLGVPVIHPGVTQCWE
                     IQKRLHVRVPRSGYGRLLSELP"
     gene            complement(1679..1897)
                     /locus_tag="VF089_07125"
     CDS             complement(1679..1897)
                     /locus_tag="VF089_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6173771.1"
                     /translation="MKITVDRTKCEGYARCVQAAPKVFQLDNKMIAEVVDTQGDTNDK
                     ILLAARLCPTKAIAIEEDGTGKKLFPEE"
     gene            complement(2038..3063)
                     /locus_tag="VF089_07130"
     CDS             complement(2038..3063)
                     /locus_tag="VF089_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173772.1"
                     /translation="MRRLLFALSIMFLGAFACETSHALAQANFFEGKAIRLIVGFTAG
                     GGYDAYTRTLARHMGKHIPGNPSMIVENMPGAGSMISANYTFKGARPDGLTIGHFIGG
                     LFLQQLLGKPGIEFDAAKFQFVGVPAQDNFVIGLAKSTGFTDVESWMASKQVVKFGGV
                     ASGSGSDDFPNILKATLGLPIQLVSGYKGTADVRLAFNSGEVAGLSNSWESTKSTWRK
                     ELESGDLKLILQATLKPHPEHPQLPMALNYAKTEEAKRLISTVARVHGPTVRPYVLPP
                     NTPADRVQTLRKAFMATMKDPEFLAEAKKANLDINAEDGATLEQNVKEILQLEPALVA
                     KLKESLK"
     gene            complement(3107..4135)
                     /locus_tag="VF089_07135"
     CDS             complement(3107..4135)
                     /locus_tag="VF089_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173773.1"
                     /translation="MALPFRWSVLIMICVLLNAPATTFAQDAFYQGKTVRIIVGTSAG
                     GGFDTYTRTLARHFGKHIPGQPAVVVENMAGAGHLIAANHMYNVAKPDGLTIGHFHGG
                     WFLHQLFKRPGLEFEATKYEFLGSPIAETRACALTKASGITSVERWLAAKTPVKIGGI
                     GGGAPDDIARMLAATTALPIQLVAGYKGTSEIRLAAESGELAGGCWTWDSIRSTWSKG
                     VQSGEAVVVLQAVSKPHRDLPNVPLAQSLAKSDEARQLLKAGIQDTAEFYRPYVAPPR
                     TPKQRVEVLRHAFDATMKDGEFLADAKKANLEIEPITGQEMERLVAGIFKLDPALVAK
                     LKSILNPS"
     gene            4438..5289
                     /locus_tag="VF089_07140"
     CDS             4438..5289
                     /locus_tag="VF089_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173774.1"
                     /translation="MIKPIALSATHMECRDLRESMAVLTDLLAFEKIAEKPGEAILKH
                     PNTHWLLVVHEAGPDAPTKQMHNHWGVRVATMEEVDRAYEYLNAHKAEYKLGHIGKPL
                     WNHGSYSCYFVEPGSNGWEIESYEAVNRKESAKAKAGGVRAPHWTSPFPEERFPGRGY
                     VPQGFTHGTLVARDMNVNRKFYTEVLSLEVHQANDHVIYVKHPNTKTYIVCAERKEFK
                     VFSPNFRNTLTVESPEAVREAYRLFSDDGDTLGVTELFPMNENGSSASFFFRDPGTNC
                     WEITSQT"
     gene            5293..5688
                     /locus_tag="VF089_07145"
     CDS             5293..5688
                     /locus_tag="VF089_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX6173775.1"
                     /translation="MIEKPLEQMGLRDLREIARRTMPAEAWHHFNGAAETKATFHRNP
                     RAFRQYVFRQRIFHDITDPDIGIELFGHELPIPAITAPVGSFSLIGSDAEREVAEGTD
                     RVGAMMFVSQAAKFNPRDWRNASKSPLVF"
     gene            5694..>6002
                     /locus_tag="VF089_07150"
     CDS             5694..>6002
                     /locus_tag="VF089_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX6173776.1"
                     /translation="MNRGREEVAEYARLAQDLGFAAVGITMDTVRPVKIGDEVPLSTK
                     DGKPRKGHKSSPKDIEWLKQQVSLPVIVKGIMSAEDAHDAVNAGADALVVSNHGGRIL
                     D"
BASE COUNT         1339 a         1609 c         1708 g         1346 t
ORIGIN      
        1 gtgggaatcg gctggaatct tagagctgag actcgcaaaa ccgcccgacc cgaagtgagg
       61 tcgtgagaag caaaaacgtt tagacgtgag cccgatccgg tgagaaaaat atcgattacg
      121 cggaaagagt gtagcgtaac cattgtcatc ccaacatatc gcgaagtcga aaatttgcgg
      181 cctcttgtga ttcgaatcag cgatgcaatg tcgaaggcga aagagtcctc tgaaataatt
      241 ttcgtggatg acgacagccg ggatggaacg gatcggattg tcgcagaact gaacgagttc
      301 ggctatcccg ttcggcttat cactcgaata ggggaaaggg ggcttagctc ggcagtcatc
      361 cgcggcttct ccgaagcgac tggcgaggct tttgtctgcc tggatgctga cttgagccat
      421 ccgccggagg cgatcccggc aattttacaa tgcctcggcg aaacaggtgt cgattttgtc
      481 ctcggcagcc gctatgttcc gggtggtagc accgacgaac gctggggtct gttgcgttgg
      541 ctcaatagta aaatagccac cgcgcttgcc cggccattga cctcggctga ggacccgatg
      601 tcgggcttct tcgcgatccg cagaacagtt tacgaaaggg cggctccact taacccggta
      661 ggctacaaga tcgcgctcga gctaatcgtc aaatgcaaat gctccatcat tagagaggtc
      721 ccgatacgtt ttgcgcagcg gcagttcggt gaaagcaagc tgtcactcgg cgaacggttg
      781 aaatatctgt ggcatctgag ccgacttctg cggtttaagc atcgataccg aatgctatcc
      841 aaagcccagt tttcaaggga tttaaagaaa ccgcagcggc ctgaaacctg acccatctcg
      901 gagaaatttt atgccttacg attgctggcg cggcatggtt ggaatgatca agccgaccaa
      961 aggctcgggc tcactggtcg agttaatcaa gatgctgccc gacggtatcg gcgtgatccc
     1021 gctttttaat aacgtgcggc atggaaagat cgaagagttt cagtcggcga tcccggcgta
     1081 tgaggaaaag atcgccgagt tagcagggga cagagtcgat ctgattcatc cggcaggcac
     1141 gccgcctttc atgctgctcg gctacaaagg cgaggcggaa ataattcagc gatgggaaca
     1201 acagtttggt attcctatct ttacttccgg cacaaatcag gtcgccgcga tgaaagcttt
     1261 gaagatcagg aagttcgtcg gcatcggcta tgatttcgat gacacccgca tcgtggcaaa
     1321 atatttcacc gaagccgggt tcgaagtcct cgagctcgga aaactgccgg gaccatggga
     1381 agaagtcggg cggctttcgt caaaagacat ttactaccaa gccaaacgtt tgtttcttcg
     1441 ccatccgaat gctgacggca tctacttcca gggcggcaag ctgcgcatac tggatattgt
     1501 cgaggagtta gagcaggatc taggggtgcc ggtaattcat cccggcgtga cgcaatgttg
     1561 ggaaatccag aaacgtcttc atgtcagggt tccgcggagc ggctacggcc gtttgttatc
     1621 ggagttgccg tagccctaac cggttcagcg cttcgaccat ctatcctcct ctgctgcatc
     1681 attcttcggg gaacagcttc ttgcctgtcc catcttcctc gatggcaatc gctttggtag
     1741 gacagagcct ggcggcaaga agaatcttgt cattagtatc cccctgggta tcaacaactt
     1801 cagctatcat tttgttatcc agctgaaaaa ctttaggcgc cgcctgcacg catctcgcat
     1861 acccttcaca cttggttctg tccacggtga ttttcataag acctcctcga gagcgttgaa
     1921 caagctcaag cgcatgcgat cgtctggtcc gcgacaaata tcaccgcacc gtcgggctgt
     1981 tggcccgaga accatgccag cattttttct ccgcgaattt gcaggtcggc aaaccggcta
     2041 ctttagagac tctttcaact tggcaacgag agcaggctcg agttggagaa tctctttgac
     2101 gttttgttcg agcgttgcgc cgtcttcggc attgatgtct aggttggctt ttttggcttc
     2161 cgcgagaaac tccgggtcct tcatcgtcgc catgaatgcc ttgcgcagtg tctgcactcg
     2221 atccgccggc gtgttcggtg gcagcacgta cggccgcacc gtagggccgt gcacgcgcgc
     2281 cacggtggag atcagtctct tggcttcttc ggtcttggca tagttgagcg ccatcggcag
     2341 ctgcggatgt tcgggatgcg gcttgagcgt tgcctgaagt atcagcttca aatcgccgct
     2401 ttccagctcc ttgcgccacg ttgacttggt cgattcccag gagttgctga ggccggcaac
     2461 ttcgccgctg ttgaacgcga gacgaacgtc ggccgtgcct ttgtagccgg agacaagttg
     2521 aatcggcagg ccaagcgtcg ctttgaggat gttcgggaaa tcgtcggagc ctgagcccga
     2581 ggcgaccccg ccgaatttca ccacctgctt ggatgccatc caggattcga cgtctgtgaa
     2641 cccggtggac ttggctagcc cgataacgaa gttgtcctgg gcgggaacgc cgacgaactg
     2701 aaacttagcg gcgtcgaatt caatccccgg tttgcccagc agctgctgaa gaaagagccc
     2761 gccgatgaaa tggccgatgg taagcccgtc cggccgcgcg cctttaaagg tgtagtttgc
     2821 tgaaatcatg ctgccagcgc caggcatatt ttcgacgatc atgctggggt ttccggggat
     2881 atgctttccc atatggcggg cgagggtccg ggtgtaggcg tcgtagccgc cgcctgcggt
     2941 gaagccgacg attaaccgga tggctttgcc ctcaaaaaaa tttgcctgcg ccaatgcatg
     3001 actggtttcg caggcgaaag caccgagaaa cataattgag agcgcgaata gtaatctccg
     3061 catagatcct ccggatcccg cgtatatatc cgtgaacgat agcgcgtcag cttggattca
     3121 aaatgctttt gagtttggcg accaatgccg gatcaagctt gaagatgccc gctaccagcc
     3181 gttccatctc ctgcccggta atcggttcaa tttcgagatt ggctttcttc gcatccgcga
     3241 gaaattcacc gtctttcatc gtggcgtcga aagcgtggcg caaaacctcg acgcgttgct
     3301 tcggagtgcg cggcggcgcc acatagggac gatagaactc cgcggtgtcc tggatgccgg
     3361 ccttgagcag ctgccgcgct tcatccgatt tcgcgagact ttgggctagc ggtacattgg
     3421 gcaggtcgcg gtgtggttta gatacggctt gcagcacgac gaccgcttca ccgctctgga
     3481 cgcctttact ccacgtcgag cgaatcgaat cccacgtcca gcagcctccg gcgagctccc
     3541 cgctctcggc cgccagccga atctccgatg ttcctttgta accggcaacg agttggatgg
     3601 gcagtgccgt ggtcgccgca agcatacgcg cgatgtcatc cggggcaccc ccgccaatac
     3661 cgccgatctt cacgggcgtc ttggccgcta gccaccgctc tacgctggtg atgccgcttg
     3721 ccttggtaag ggcgcaagcg cgggtttctg cgatcggcga tccgagaaat tcgtatttcg
     3781 ttgcctcgaa ttcgagtccg ggacgcttga agagctgatg gagaaaccag cctccgtgaa
     3841 aatgaccgat cgtcaggcca tccggtttgg caacgttata catatgattg gcggcgatca
     3901 ggtggccggc gcctgccatg ttctccacta ccaccgcggg ctgtcccgga atatgtttgc
     3961 cgaagtggcg cgccaaggtg cgtgtgtagg tgtcgaagcc gccgccggcc gaagtgccga
     4021 cgatgatccg aacagttttc ccctgataaa aggcgtcttg agcgaaggtc gttgccgggg
     4081 cgtttagtag gacacaaatc atgatcagaa ctgaccaccg gaatggcaac gccatgctcg
     4141 cctcctgtaa cctcaagctg cgcggaatct atggaaaaca aaactttgtg tcaagctgat
     4201 tattcttcac aggatgatcc agcactgcga gtgatgggtt gaacaccgaa gcaactggtg
     4261 tgcttagggt gagttaccgc accgatcatc agaaccgtgc gagggttgct taacaggacg
     4321 agcccatctc gtgctcggat ttcctcgttt gacagggtag tcgtaccgct gtaaattttc
     4381 gtaaacatgt ttgttaaggc ggcgagcgat caaatggaat ccacacgagc taggggcatg
     4441 atcaagccca tcgcgctttc ggcgactcac atggagtgcc gtgatttgcg agaatccatg
     4501 gccgtgctga ccgatctttt ggctttcgaa aaaatcgccg agaagccggg cgaagcgata
     4561 ctgaagcatc ctaacacgca ttggctgttg gtggttcatg aggcgggccc ggatgcgcca
     4621 accaagcaga tgcataatca ttggggcgtg cgcgtagcga caatggaaga agtcgacaga
     4681 gcctatgaat atttaaacgc gcataaggcc gagtacaaat tgggtcatat cggcaagccg
     4741 ttgtggaatc acggatcgta ctcctgctac ttcgttgagc caggaagcaa cggctgggaa
     4801 atcgagtcct atgaagccgt caatcgaaaa gaatcggcca aggcaaaagc aggcggggtg
     4861 cgcgcgccgc actggacgag cccattcccc gaagagcgtt ttccgggccg cggttacgtg
     4921 cctcagggat ttacccatgg taccctggtg gcccgtgaca tgaacgtcaa tcgtaaattc
     4981 tacacggaag tgctaagctt ggaggtccat caagccaacg accacgtgat ctacgttaaa
     5041 catcccaaca ccaaaaccta catcgtttgt gccgagcgca aggagtttaa agttttttcg
     5101 ccgaactttc gcaatacctt gacggtcgag tcgcccgaag cggtcagaga ggcatatcgc
     5161 ctattttcgg acgacggaga cactctaggt gtcactgagt tgttcccaat gaatgagaac
     5221 gggtcgtcgg cctctttctt ttttcgcgac cccggcacca actgctggga aattacctca
     5281 cagacttagc ttatgatcga aaaaccgttg gaacaaatgg ggctccgcga tctccgcgag
     5341 atcgcccgac ggaccatgcc ggccgaggct tggcatcatt ttaacggggc cgctgaaacc
     5401 aaggcgacct ttcatcgaaa tccccgtgcg ttccgtcaat acgtgttccg gcaaaggatt
     5461 ttccacgaca taaccgatcc ggacattgga atcgagctct ttggccacga gctgccgatc
     5521 ccggctatta cggcacccgt gggcagtttt agcttgattg gcagtgacgc ggagagggaa
     5581 gtcgccgaag gcaccgatcg tgtaggcgct atgatgtttg tcagccaggc ggctaaattc
     5641 aatccgcggg attggcgcaa cgcatcgaaa tcaccactgg ttttttagcc tatatgaacc
     5701 gcggccgaga agaagttgcc gaatacgcca ggctcgctca ggacctcggc tttgcggcag
     5761 tgggaatcac catggacaca gtgcgaccgg ttaagattgg cgatgaagtg cctctgtcaa
     5821 ccaaggacgg taagccgagg aaggggcaca agtcttcgcc caaagacatc gaatggctga
     5881 aacagcaagt ctcattgccg gtgatcgtca aaggcatcat gagcgcggag gatgctcatg
     5941 acgcggtcaa tgccggcgcc gatgcgctgg tcgtttccaa tcacggcggc agaatcctgg
     6001 at
//
LOCUS       DASUTG010000313         6564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_492890, whole genome shotgun sequence.
ACCESSION   DASUTG010000313 DASUTG010000000
VERSION     DASUTG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492890"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VF089_07155"
     CDS             complement(<1..519)
                     /locus_tag="VF089_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173777.1"
                     /translation="MSPPSESATGSRRLLAAVSALHHRNYRLYWFGQLFSVLAQNMEA
                     VAQSWLVLEMTNSPLLLGLTGLTFSVPTIALTLLGGAVADRTDRKRIMVYSQIGSVVM
                     FLLLAVLVITERVALWQVMTLAFFSGCVKAFDRPSRMALLPQMVPKHDIPNAVAIGGT
                     IWQLNKLVGPALA"
     gene            666..2018
                     /gene="gor"
                     /locus_tag="VF089_07160"
     CDS             666..2018
                     /gene="gor"
                     /locus_tag="VF089_07160"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX6173778.1"
                     /translation="MAQYDYDLFTIGAGSGGVRGSRVAASLGAKVAVAEESALGGTCV
                     NVGCIPKKLLVYASHYSDDFRDAAGFGWTVGPRSIDWAKLIANKDSEIRRLNGVYRKL
                     LDDAAVEIIEGHAQVLDPHTIGVNGKRYTAKYILIAVGGWPSIPIFPGSEHVITSNDA
                     FYLPELPEKVVIVGGGYIGVEFAGIFHGLGAETAQLYRGELFLRGFDNDIRLTLADEM
                     LKRGIDLRFKTDVAKVEKHGDILKATLIDGSVIEANQILYATGRVPKTRGLGLENVGV
                     ELRQSGAVVVDPYLCSSVESIYAIGDCTDRLMLTPVAIAEGTAVANTLFNNRPIRLDY
                     ENVATAIFSAPNCGTVGLTEEQARRREFEVDIYMSSFRPLRHTMTGSPEKTMMKLVVD
                     RASERVLGCHMVGPDAGEIIQGLAVALNCGATKTQFDTTIGIHPTAAEEFVTMRTKTR
                     "
     gene            2266..3012
                     /locus_tag="VF089_07165"
     CDS             2266..3012
                     /locus_tag="VF089_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6173779.1"
                     /translation="MDRATFREQLLVVMEGKDHWAWPAFTSGAVRKDLLHHHLEQEYA
                     TYVRDFPVMVGRAYVRCPIPQVRRSLAENLYEEETGGLVAGSPHPELFLEYARGLGMD
                     LSRFANVSLLPAAKSYRQYLDDATQRYGWEIAAAVVTIFVEGSKHERRAIEPTVAVDP
                     IPLEEHPLVKHYGMPIEHLALTKAHRQVEGNHRAAAWAAILDYVKPTRRGTVVRAMQE
                     ALTSWLRYRDAVAEICGVDCKVHDYKSAVA"
     gene            3672..4148
                     /locus_tag="VF089_07170"
     CDS             3672..4148
                     /locus_tag="VF089_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173780.1"
                     /translation="MQKMRTLVWLGVGFFSALLLSDLAFADSGRSYGAGRGAIRESNR
                     DVQRSRAELRKDFRELRRDRAELRSDIRRGAPRHEIARGKSEIRRDLREIQRDRRELR
                     RDNSELRRDLRRFGYDNHRYSRGRNQGNYGWWNNRDRWDRRPSGWSSNHRDNRYSW"
     gene            complement(4534..4809)
                     /locus_tag="VF089_07175"
     CDS             complement(4534..4809)
                     /locus_tag="VF089_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173781.1"
                     /translation="MIAKLPGAEDVMESIGASKSSGTFGGGAMLGGLTSAMGQSGGAL
                     AALHGSGLSASQAGPFVKMLVDYALQKAGPEIVERVLGKVPASKSMM"
     gene            6362..>6564
                     /locus_tag="VF089_07180"
     CDS             6362..>6564
                     /locus_tag="VF089_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173782.1"
                     /translation="MKAARSILFVDDDRLVRQLFTRLLVKRGYRVSWAADRQQAVALF
                     SKQQFDVVIAELHLRSSPDGLDIL"
BASE COUNT         1544 a         1726 c         1806 g         1488 t
ORIGIN      
        1 ggccagcgcc gggccgacaa gcttgttcaa ctgccagatc gtgccgccga ttgcgacggc
       61 attcggaata tcgtgcttcg gcaccatctg cggcagtaga gccatccggc tgggccggtc
      121 gaacgccttg acgcaccctg aaaagaaggc caaggtcatg acttgccaga gcgccacccg
      181 ctcagttatg accaggaccg ccagtagcaa aaacatcaca accgagccga tctgcgagta
      241 aaccatgatt cgcttacggt cggtgcgatc ggccaccgct cccccgagga gtgttagcgc
      301 gatcgtagga accgagaatg ttaaccccgt aaggccgagg agaagcggcg agttggtcat
      361 ttcaagcacg agccaactct gggcgacagc ttccatattt tgcgctagca cggaaaagag
      421 ctggccgaac cagtagagac gataattgcg atgatgtagc gcgctcacag ccgcaagtaa
      481 acgtcggctt ccggtggcgc tctcggaggg tggtgacatg gtctagtggc aaaacacgga
      541 tcgagtaagt gcggcattct cgatcaaatc attgacagtg tcaaaggcgt agtgaagcag
      601 tctgaagttg aggaatagga atggaccttt tatcatagcc ggacattccc tgaaaggcgg
      661 ttttcatggc ccagtacgac tatgatttgt ttaccatcgg cgccggctcc ggcggtgtcc
      721 gcggcagccg cgttgctgca tcccttggcg cgaaggtggc agttgcggaa gaaagtgccc
      781 tcggcggcac gtgcgtgaac gtcggctgca tcccaaagaa acttctggtt tacgcctctc
      841 attactcgga cgactttaga gacgctgccg gattcggctg gacagtaggc ccccggagca
      901 tcgattgggc gaaactgatt gccaataagg atagcgagat tcggcggctc aacggcgtct
      961 accgaaagct gctcgatgat gccgctgttg aaataatcga ggggcacgcg caagtccttg
     1021 acccgcatac cataggagtg aacggcaaac gttatacggc gaaatatatt ctgattgccg
     1081 tgggcggttg gccatcgatc ccgatattcc ctggatccga acacgtaatc acctctaacg
     1141 acgctttcta ccttcccgag ctgccggaaa aagtggttat cgtcggcggt ggctacatcg
     1201 gcgtcgagtt cgcggggatc tttcatgggc ttggcgcaga gacggcgcag ctttatcgcg
     1261 gcgagttgtt tctgcgcggc ttcgacaacg atatccggct cacgctggcg gatgagatgc
     1321 ttaagcgagg gatcgacctt cgcttcaaga ccgatgtcgc caaagtcgag aagcatggcg
     1381 acatactgaa ggccacgctg atcgatggca gtgtgataga ggccaatcag attctctatg
     1441 ccacgggtcg cgtgccgaag actcggggtt tgggcctgga aaacgtcggt gttgagttaa
     1501 ggcagagcgg tgcggttgta gtcgatccgt atttgtgctc gagcgttgag agtatctacg
     1561 caatcggcga ttgcacggac cgcctaatgc tcactccagt tgctatcgcc gagggcaccg
     1621 cagtggcgaa cacgctcttc aacaacaggc cgatcaggct cgattatgag aatgtcgcca
     1681 ctgcgatttt cagtgcgccc aattgcggca cagtcgggtt aaccgaagag caggcacgcc
     1741 gtcgtgaatt cgaggttgac atctacatga gctcgttcag gccgttgcgg cacacaatga
     1801 ctgggagtcc ggagaaaacg atgatgaaac tagtagtgga ccgcgccagc gagcgagtgc
     1861 ttggctgtca tatggtcggg ccggatgcgg gggagatcat tcagggattg gccgtagcgc
     1921 tcaactgcgg cgcgaccaaa acgcagttcg atacgaccat cggaatccat ccgacagctg
     1981 ctgaagagtt cgtcacgatg cggaccaaaa cgcgatagga accgcatcgg tcttcactgg
     2041 cttatctgcc cgcgggcatg ccaattctgt cccatttcgc gcccatgccc tgctcacatc
     2101 tcgctttcga ggagtcattc gcgtcgcgag cggtgtgctc acacaacacc ggcaaccttg
     2161 ctattgaact ctgaagttcc cgaatatgtc gcaacgccgc tacccgccgc ggcatggtag
     2221 ttgctgtctc gcaccttgcg aagagtatcc gcaaaggggg gcaacatgga tcgagcaact
     2281 ttccgcgaac agctcctggt tgtgatggaa ggcaaggacc attgggcttg gccggcattt
     2341 acctccggcg cggttcgcaa agacctgctc catcaccatc tcgaacagga atatgccacc
     2401 tacgtccgcg attttcctgt gatggtcggg cgcgcttacg tgcgctgtcc gattccgcaa
     2461 gttcggcgca gtcttgcaga aaatctttac gaagaagaga ccggtgggct agttgccggt
     2521 agtccgcatc ccgagctgtt tctcgaatac gcacggggcc tggggatgga cctaagccgt
     2581 ttcgccaacg tatcgctgct accggcggca aaaagctatc ggcaatacct tgatgacgcg
     2641 acccagcgtt atgggtggga gatcgctgcg gctgttgtca ccatctttgt cgaaggatca
     2701 aagcacgaac ggcgcgcgat agaaccgaca gtagccgtcg atccgatccc tttagaggag
     2761 catccgctgg tcaagcatta cggcatgccg atcgaacatc tggccctcac caaagcgcac
     2821 cggcaggtgg agggaaacca tcgggctgct gcatgggcgg cgattctaga ttacgttaaa
     2881 ccgacaaggc gcgggaccgt ggtcagagcc atgcaagaag cactaacaag ctggctgcgc
     2941 tatcgagatg cagtggccga gatctgcggc gtggattgca aggttcatga ctacaagagt
     3001 gccgtagcat agcgaacgtg ctctgtgatc tcgtactatg ttccatcaca taacaaaaca
     3061 aaactagtga cgagtggaat ggcttaaacc gcagatgtca gtacggccac gagtcgttta
     3121 gttctactct cctgcgaacc gatagtgtcg ccgacataac tcatctttac ctacatcggt
     3181 ctcattgacc ggccgatcgc gctgctcagc tgatagccat agaccgcagt accaacagat
     3241 taccttcgta tcgccctcag agatcgcatg aaatcccagt gccggtttaa atgactggga
     3301 cagactccgt tatgcttgct caaatcctga gctatcctcg atacgttatt ttgctgtcag
     3361 taagagcgtg agcacgttac ggctttgtag cgcgaccccc tgcgctcgcg gctgataagg
     3421 ccaatagagt tgacgtgtcc ggctagtagc acatgtcgct cgattttatg gttcgagtac
     3481 ccgcgccgct gttctcttac atgagctagc aggcgaatgt atttagccaa gtgagtgagt
     3541 tgtcgtgact gtcaggatct atttacaaca ggtgcttcac tgtagaacca aggcactagc
     3601 gactgggtga tccgttgcct ggttaacatt tgttatgtga gtacgtctaa ggcgtaaaag
     3661 gggagaaagc catgcaaaag atgagaactc tggtctggct aggcgttggc ttcttttcag
     3721 cgctactgct cagtgatttg gctttcgccg atagcgggcg ctcgtacgga gccgggcgcg
     3781 gcgcaatccg ggagagtaac cgtgatgttc aaaggagtcg cgcggagttg cgcaaggatt
     3841 tccgtgaact caggcgtgat cgcgccgagc tgcgcagtga catacgacgc ggagcgccgc
     3901 gccacgagat tgcccgcgga aaatctgaaa tcaggcgtga tttgagagaa atacagcggg
     3961 accggcgcga attgagacgt gataattctg aactgcgccg ggatctacgc aggttcggct
     4021 atgacaatca tcgatatagc cgcggccgaa atcagggaaa ctatggttgg tggaacaatc
     4081 gcgatcgttg ggaccggcgc ccgagtggat ggtctagcaa ccaccgtgac aatcggtaca
     4141 gttggtgaaa caatcacagc ttcgaccggt aggacttaat tgtagccagg gaatgaactt
     4201 cgcagctagc attttcccag cgcttactgg ttagaggcgg ctttaagccg cctctaacca
     4261 ctcatttatc aggttcaaag gtaaatttac gactgataaa ccggatagta cactacgtga
     4321 acatcatcgc atcaggcctg ggtttccgtc gcataaccca tattcaccgg agagttgaaa
     4381 aggcgaatcg tttctaccgg ctgtttttga gccagaatat ttgacgcgac tacagcgccg
     4441 ggtaatgtag tatagaaacc agacacctgc gcgttggatc agattcctac aatcaggtct
     4501 ggctctctgc cacctccgca gcggtagtaa aaatcacatc atactttttg atgccggtac
     4561 tttaccgagg actcgctcaa caatctccgg gcctgctttt tggagcgcat aatcaaccag
     4621 cattttcaca aatggtccgg cctggctcgc gctaaggccg ctaccgtgta atgcagccaa
     4681 ggcaccgccg ctttggccca tagccgaagt tagtccgccc agcatggcgc cgccaccaaa
     4741 agttccgctg cttttgctcg cgccgatgga ttccatcaca tcttccgcgc cgggcagttt
     4801 agcgatcaag actgcgcttc gttgccgcct tgattctttg gaaaattgag caagcctcca
     4861 gtcgcggact ttgcctgacc ggtgactact agtgatctat tgcccgccac aactgctagc
     4921 gcgtatcggc tactctggta agtctctaag atctgtgatc gtaaaaatca ttgtccatat
     4981 ccagtctgaa cactgcgctt gcacgaaagc aattccgatc ctttctgtac gaatcgttcg
     5041 gcgattcgcc cacgtctttc ccaattccgc attccacgtc gtctcagcct tctttcaaat
     5101 ccagcgtggc actttcttca tattattgat gtaaggcttg ccgagcctag ttgctaattc
     5161 ttcttcatcg ccgatcactc ggcgctgaaa ccacaacgca attgcgatgg tgatcacaga
     5221 gccaagccag aattgtagtg tcaaactcaa tccgagaagg taactcagat ggcccaggta
     5281 catcgggttg cgggtgtagg cgtaaggtcc ggttgaaacg acgcgatccg gcgggtttca
     5341 aggcccggac cgccgccacc atgtttaatg cgataacgac cacagagccg gtattgcaga
     5401 tagccccagg ccataagcgg caagaacatc ggctccatat cgaggggcct ctgttccaga
     5461 taagctccca gactccgagc accggataga tgatgaaaag tctaaccggt gtgcgactaa
     5521 gaaatcttcg gtaactccct actccggagg agtgaaggca gctttagcta tgtttagatc
     5581 cgcaccttaa tcaaaagggt gatctcgaat ttatatcaag accaattggt tcaagtatga
     5641 gaaacccgct cggcatcgat cggtttgcag ctctcgatcc cactgttagc ttctagttcc
     5701 cgcggattaa atcagacgaa tatggttctt tggacgttcc cgatattctt tatgcgctcc
     5761 agctttgatc gattttttct tctgaggcac cgctgctata tcgtttcgat tccaatcggt
     5821 tctctctcaa ttccaacaca aacaccgcag ttccgaagcg cttatcatca gagaagaagc
     5881 ggtacgacta agaggcccgt ctctgtgaac tcccttgtca catgcttgag ctttgtaacg
     5941 caaaaagtgc tcgagccgcc aagcatgtca agacagaaat ttagctggct gcttgaatcg
     6001 aaatccctgt cattggccgg ctcagaccaa gcgctgtagg cataaacata aatttagcca
     6061 ggatcaaaag tataagcgga gagtggggaa caacaaggcg tcgcggcgcg gtaggtgtct
     6121 tgtgccgttc agccgctcgc acttatcggc caagagttct cctcgctgaa caggagctgg
     6181 acagaaaaga atttctggac aacgtcgacg agattggcca tgccgaatta ctggctgtgc
     6241 cgttagagct tacctatcta ctaagttcgt aaaggattct gcaaccggaa gcgaggagat
     6301 aaattggcag attagagatt tttgttgtaa atgcggtagc taatagatac cctcggagtc
     6361 gatgaaagcc gcgcggagca ttctgttcgt tgatgacgat cggcttgtcc gccaactgtt
     6421 caccaggctg ctagtgaagc gaggatacag agtcagttgg gcggcggatc ggcaacaagc
     6481 ggtagcgctg ttttcaaaac aacaatttga tgttgtaatt gccgagcttc atctgaggtc
     6541 cagtccggac ggattggata ttct
//
LOCUS       DASUTG010000314        12870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1234724, whole genome shotgun sequence.
ACCESSION   DASUTG010000314 DASUTG010000000
VERSION     DASUTG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234724"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF089_07185"
     CDS             complement(<1..571)
                     /locus_tag="VF089_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6173783.1"
                     /translation="MRPAPIKLPNDAKIAVSFFVAFEAFEKYSQYREGGDKPDYASLA
                     YGEYGGKAGIWRIMDVLARNQVKGTIDTNGRIAEDFPDALKELHAGGHEIVGHGWVND
                     TSLVSLEPAKEKELIKRTLDSLASVTGKRPVGWVSPGHRINDYTFAHFVNEGIIWTGD
                     LPGPDIPQHRVINGKPLVIMPRLDYANDLS"
     gene            complement(795..2078)
                     /locus_tag="VF089_07190"
     CDS             complement(795..2078)
                     /locus_tag="VF089_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6173784.1"
                     /translation="MEGILFPHGIPEGREELNDYRERGGYEALARAIKMPAEEVVQQV
                     SDAGLRGRGGAGFPTGKKWAFTREAPEQPRYLIMNGGEDEPGSKKDRVLMENIPHLVI
                     EGVILSAYAIGAEKAYLYINAEYETAIKSITDALTEAKTEGYWGGNILGSDFSLDIEI
                     VPAPHNYVAGEDTAVIEVIEGKKAWPRQKPPFPVTVGLFGKPTSVNNVETLANVAPIL
                     LHGAEWYRKYGTVESPGTMLFALDEDVNRPGVYELPFGTPLRYLIEECGGGLKNGKKI
                     KAIMPASPSCAFLPPDRIDTPLDHNSMREAGAGIGCGVIRLVSEGECIVEEVLSIADF
                     FAAESCGQCPACRMETSTLAMMMKKVQQGQGGQPILEQFGKILAFNKGKGFCNLINMP
                     GPPIESAIKLFPGDFEAHLTTGKCPVNLQAAEKTS"
     gene            complement(2082..2396)
                     /locus_tag="VF089_07195"
     CDS             complement(2082..2396)
                     /locus_tag="VF089_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX6173785.1"
                     /translation="MCLVCQNIDCKSRGSEQLMGELEKRVAAKGLDDVEVKPYMCFGA
                     CQEGPNIVLYPEKSWYANVKVEDLDEIAEHIAGGPHVKRLDTIDPSLKELIYQLLDTG
                     VV"
     gene            complement(2471..3577)
                     /locus_tag="VF089_07200"
     CDS             complement(2471..3577)
                     /locus_tag="VF089_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6173786.1"
                     /translation="MQKIANFDDWIDYFRFWQKDIGYDPALLGDYKFETKLGELHSSE
                     IEFGDFKGQPKWQRIGQVPNQAIRDALMNLIVYQGDTEFASVEQQKNLLDTAPTEYDR
                     KALTRVNSEEMRHGWQMCYLLVNYFGDSGKLEARKLLERRAFRGDRLLGSFNAPVKNW
                     LDFFTYTEFVDRDGKYQLTMLSHSAFAPLAESVTAMLKEEHFHMFTGHTGLTRVLRAG
                     QIPVAIVQKYFNKWLSTAYDLFGTDHSSSANWAYVWGLKGRYDEHEATVAADKDRLND
                     LARDHYMAESSKLIDQLNQLIPEGQPKLSAPDLKFNRSIGEHIGKTFSVTGELLSPEA
                     YKKHLTEALPTAEDEQLLNDIMKGKEWVSQIQMN"
     gene            complement(3689..6088)
                     /gene="ptsP"
                     /locus_tag="VF089_07205"
     CDS             complement(3689..6088)
                     /gene="ptsP"
                     /locus_tag="VF089_07205"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX6173787.1"
                     /translation="MAKLKKTGRELHPHLTILEDISTLITHSHDLQDTLQNIVTTVRD
                     RMEAEVCSIYILDPQKKRLTLRATMGLDPESIGKVSMAIGEGLTGLVIERMEPVMVVD
                     ALAHPRYRYFPETGEERFHSFLGVPLFNNKSPLGVLVVQTSRRRQFSRDEIRLLITIS
                     AQAASIIVQARLLESLRNKERERKEYQKRMVDALRKLRSYEGRRRDKTSKTSHKWRGR
                     LVGLAASPGFGRGRAFVLNPRMELTAIEKKRARNPQREIERFRGAVERGIEQINVVKQ
                     RMSNLISREEGAIFDVYRLILEDPAIIQQIEAQIRKENYVAEYAVRLVFEQYLSSISQ
                     IQDDYLRERTTDVKDAAQRLLENLAGTPDQHIDVPSDAVLVAEDLSPADLSMLEGDHF
                     RGIVLSTGGVTTHASILAKSFEIPSVVAIEGLMDIVHQGDAVIVDGNSGVLYVNPNPE
                     VAREYDRLERDYAALNRELEELKDEPAETIDGHRVSLYANIGLLSDIAFAHLHGAQGV
                     GLYRTEIPFLAHRDFPSEEEQYSLYKRVVEGMAGKPVTVRTLDIGADKYPSYMRSTKV
                     EPNPFLGWRSIRISLDVEEIFKTQLRAILRAGTLGRVRLLIPMISSLEEVLKVKELLA
                     EAKDELQRESTPFDLNMELGIMVEVPAAVQLAERLLREVDFLSIGTNDLIQYILAVDR
                     SNRKVANLYEPLHPAVLAALYSTIQAGKRDGKRVGMCGEMAGDPIYALVLLGMGLEEF
                     SMGSLFVPVVKKTIRSVTYEAAKSMARIVLEMDTAAEIKRYLFEQMRELGMVELMEMY
                     H"
     gene            6383..6793
                     /locus_tag="VF089_07210"
     CDS             6383..6793
                     /locus_tag="VF089_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007145401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173788.1"
                     /translation="MSQIDGIKGMRHIALKVRDIAKAKSFYQNMFGMEVVWEPDPENV
                     YLSSGYDNIALHQVAAGFAATAAEQHLDHFGFIVESIERVKELEEQFRSAGVTIVHPF
                     KIHRDGSASFYCADPDGIVIQMLYEPQLSQIAPN"
     gene            7003..8004
                     /locus_tag="VF089_07215"
     CDS             7003..8004
                     /locus_tag="VF089_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173789.1"
                     /translation="MATKLKFGLLLPHFGEYASAESCIEGSKRAEEYGFDSVWVRDHL
                     VFEPHGMEGNDNTHIEGLLILAAIASVCKKLTLGTGTMISHRHPIHLAQAVAGLSTLC
                     DSNIIMGLGLGTFPHEFEAAGYKKVTLQDRANIAKANALLCRRFWAGEKIEHYKDDYY
                     EFKDVELRPTPSKNIPIWYGGGTPASCRRASDYCDGWMHGRIPTATFNKMVKYLEEQC
                     KLAGRPMVTTGAIPITSIDKNRDVALGKVNTKGLINEGNSPSKKTWVKPKSGTFSTVE
                     DIEGLLMAGTPADIARDTKRYEEAGLNHIVFDLRFRYADWYEQIDYLGQEVLPKVRA"
     gene            8198..9118
                     /locus_tag="VF089_07220"
     CDS             8198..9118
                     /locus_tag="VF089_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6173790.1"
                     /translation="MRTEKVNFFSEGIKLAGVLYLPDSDKDKPFAGVVQGPGFLGLKD
                     AKHYIMMFEKLCAAGYACLCFDYRGWGDSEGNDRGWVVPKWQAEDIRNAITFMQTRAE
                     IDPDRIATYGSGGTGGGNAVYVAAIDQRVKCCVSYLGVSNGRDWLHSMRREYEWIDYL
                     KSIEEDRKKRVTTGSGAMVSAREGGIMVQTPERQTTAIKKDVADKIPDAMPLACAEAI
                     LEYSPIDVVDKISPRGVLFIAVEGDAVTPEQQSFDLYEKAREPKKLVLYKKTTHYGIY
                     NDYFDDVAANIVDWYNRHLKYNKVDITENH"
     gene            9128..10150
                     /locus_tag="VF089_07225"
     CDS             9128..10150
                     /locus_tag="VF089_07225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6173791.1"
                     /translation="MHKISFGVRVPNSGPLSSVENIVKATKTAEELGFDAIWVHDHVV
                     WSSEMHRHHISSGAAEALTDTQEANFFEATTILSYLAAETKTITLGVACLVMPCRNPI
                     YAAKQYSTLDHLAKGRLLVGVGLGSKATRESDEFGVFGVPYERRGDRTDEYIEAMKAV
                     WTQPLASYKGEFIEFKDAEVYPKPVQKPHPPVWVGGWMKLAAKRAGKYGEGWIPGWLS
                     PKEMKVGCDILAQTAKDNGRDPKKITIAVEKLATIAKTRDEGLQLALPTLKTSSESYE
                     RDIDSMQFALDRHIFGSVDDVRRRVDEFIENGVQHFELKIIYPTMDSLERQMTLWAES
                     VLPHYE"
     gene            10155..10643
                     /locus_tag="VF089_07230"
     CDS             10155..10643
                     /locus_tag="VF089_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6173792.1"
                     /translation="MRNVALKFHLNGRPVELECEAEETLAEILRNQLNLNGTKVSCEV
                     QVCGACTVLVDDLPVSACTYLAYEAHGKSVTTIEGLEKPDGTLHPIQQAFIDDFAFQC
                     GFCTPGMIMSAKALLDENPTPSREEIIHHMDGNICRCTGYVPIVAAIERAAQAMSRDS
                     KK"
     gene            10871..>12870
                     /locus_tag="VF089_07235"
     CDS             10871..>12870
                     /locus_tag="VF089_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6173793.1"
                     /translation="MSAEKKTDFYVVNHSVPRRDGRVKVTGKAQYVADLKLIGMAYAK
                     VLRSPYAHAKIISIDTSRAAARPGVFCVVTGNDLEGLNPYYGHAVKDHPLLAIDKVRY
                     AGEPVAAVVAIDERTAFEALEFIDVKYQELKAVFTPQEAMAKDAPLLHERQFEAGALR
                     GFEGEVTAGKGSNICQKHEVRWGNVEEAFKNAAAVVEADYYFPMTYAYAMEAYVAVAD
                     VTDQGVTIYSSAQHPFMVRHDLKTVFNLPVSKVRLIVPFVGGGYGSKSYTKIEPLVAA
                     CSWKAKRPVKLQLTIEEAMLTTRSDDAYTWMRTAVDENGKIIARQAKIYMNTGAYAEN
                     SPLVVEKSSNRCVGPYAIPNVLIENCSLYTNTVPASSYRGFGCAQVTLPGESQIDELA
                     AKIGKDPYEFRLANAAKPGEEFFPGMRPFDGTLKEDLDTAANAIGWKDPLPSGHGRGV
                     ACSGSDAGAYPLTSTAVRVHADGSVTIMTGSTELGQGSHTVIPQIAAEELGVDFDKVN
                     VVSSDTAITPYDRSTGASRTTTLMGSAVLEASREAIQQMVVMAADVLKVKVEDIQVVR
                     GGVRCGEAQLNWSEVISKFYALPDGEVIGRSYIRKSGKFAALPVFWEAAATGVEVSVD
                     QETGKITMEKLATVGDVGLAINPALAEGQVVGAATMGMGIGL"
BASE COUNT         2963 a         3508 c         3507 g         2892 t
ORIGIN      
        1 gcgaaagatc gttggcgtaa tccagccgcg gcatgatgac cagcggtttg ccgttgatca
       61 cccgatgctg aggaatgtcg ggtccgggta aatcgccggt ccagattatt ccctcgttga
      121 cgaaatgcgc gaaggtataa tcgttgatac gatggcccgg gctgacccaa cccacagggc
      181 gcttgccggt tacggacgca aggctgtcga gcgtccgctt gatcagctct ttttccttgg
      241 ccggttctaa cgagacaagc gaagtgtcat tgacccaacc gtggcccacg atctcatgac
      301 caccggcatg aagctctttc aatgcatcgg gaaaatcttc cgcaatgcgg ccgttggtat
      361 cgatcgtccc tttgacttga ttacgtgcca ggacatccat gatccgccag atgccggctt
      421 tgccgccgta ttcgccgtag gcgagagatg cataatccgg tttgtcgccg ccttcacgat
      481 actgcgaata cttttcaaac gcctcgaaag cgacgaagaa actgacggcg atttttgcat
      541 cgttgggtaa tttgattggt gcgggtcgca tcctttgtac ctcgtagtta aaccgaggga
      601 gatcctatca aggcgttgct tgaaccgtca acctgaattg gcgcgggcgg aaataaaagg
      661 gcgagtttag cagtttgcta aaaaatttgc tttgcaggct gctcaaagga cctcagagct
      721 aaggcgcgtg aagctcgatg agctgaggcg tacgcttcag tacggcgaag cgaggcgggt
      781 gagcgtaacg aagcctatga ggttttttca gcagcctgca agtttaccgg gcatttcccg
      841 gtagtaaggt gggcttcgaa atcaccggga aagagtttga tggcgctttc gataggcggg
      901 ccgggcatgt tgatcagatt acaaaagcct ttgcctttat tgaaggccaa aatcttgccg
      961 aactgttcta gaatcggctg accgccctgc ccttgctgca ccttcttcat catcatcgcc
     1021 agcgtgctgg tctccatgcg gcatgccggg cactggccgc aggactcggc ggcgaaaaag
     1081 tcggcgatgc tgagaacctc ttcgacgata cactctcctt cgctgaccaa acgaataacc
     1141 ccgcagccga tgccggcgcc ggcctcacgc atcgagttgt ggtcgagcgg tgtgtcgatt
     1201 ctatccggcg gtaggaatgc gcatgatggc gaggcgggca tgatcgcttt gatcttctta
     1261 ccatttttca aaccgccgcc gcattcttcg atcagataac gcagtggggt gccgaacggt
     1321 aattcataca cgcctggccg gttcacgtct tcgtcaaggg caaagagcat ggttccagga
     1381 ctttcgacgg tgccatactt tcggtaccac tcggcgccat gcaaaaggat cggagcgacg
     1441 ttggcgagag tttcaacgtt gttcaccgac gttggcttgc caaaaagtcc taccgttacg
     1501 gggaacggcg gcttctgtcg gggccaagct ttcttacctt cgatgacttc gataacggcg
     1561 gtatcctcgc cggcgacata gttgtgcggc gccgggacaa tctcaatgtc gaggctgaaa
     1621 tcgctgccaa gaatattccc accccaataa ccctcggtct ttgcttccgt caaagcatcg
     1681 gtaatgcttt tgatcgcggt ctcgtattcg gcattgatgt aaagatacgc tttctcggcg
     1741 ccaatcgcgt aagcggatag gattacgccc tcaatgacca ggtgtgggat attctccatc
     1801 aacacgcgat ctttcttgct gcctggttcg tcttctccac cattcatgat gaggtagcgg
     1861 ggttgctccg gcgcctcacg agtaaacgcc cacttcttgc cggtgggaaa tcccgcacca
     1921 cctctcccgc gaagtcccgc gtcggagact tgctgcacga cctcctccgc cggcattttg
     1981 atggcccgcg ccaacgcctc atacccaccg cgctcgcggt agtcgtttag ctcttctcga
     2041 ccctcaggaa taccatgggg gaaaagaatg ccttccatcg gttaaaccac tcccgtatct
     2101 agaagttggt aaattagttc tttgagcgaa ggatcgatag tatccaagcg tttgacgtgg
     2161 ggtccgccgg cgatgtgttc agcgatctcg tccaaatctt cgactttcac gttcgcatac
     2221 cagctcttct cgggataaag gacgatgttg gggccctcct gacatgcgcc aaagcacatg
     2281 taaggcttta cctcgacatc gtccagccct tttgccgcaa cccgtttctc gagctcaccc
     2341 ataagctgtt cggagccgcg agacttgcaa tcgatattct gacaaacgag acacatccgc
     2401 ttcactgcgc tctccttccg gtttaaccgt cagcagccat caatccgaga agcggcacgc
     2461 atccgcccgc tcagttcatt tgaatctgcg aaacccactc tttgcctttc atgatgtcat
     2521 tgagtagttg ctcgtcttcc gccgtcggca gggcttcggt taagtgcttc ttgtatgcct
     2581 ctggggacag gagctcaccg gtgacgctga aagttttacc gatatgctca ccgatggagc
     2641 ggttgaactt caaatccggc gccgaaagtt tgggctgtcc ttccggaata agctggttga
     2701 gctgatcaat taacttcgag ctttccgcca tataatggtc acgggccaaa tcatttagcc
     2761 ggtccttgtc ggctgccacg gtcgcttcat gttcatcgta tcttcccttc agcccccaga
     2821 cataggccca gttcgccgag gatgaatgat ccgttccgaa cagatcataa gccgtggaca
     2881 accatttgtt gaaatatttc tgcacgatcg caaccggaat ttgtcctgcg cggagcaccc
     2941 gggtaagtcc ggtatggcct gtgaacatat gaaagtgctc ttccttgagc atggccgtca
     3001 cgctctcagc cagcggcgcg aacgcggaat gacttagcat ggtcagctga tatttgccgt
     3061 cccgatcaac aaattccgta taggtaaaga agtcgagcca gttcttcacc ggagcattga
     3121 aagatccgag tagccggtcc ccccggaacg ctcgtcgctc gagcagttta cgcgcttcca
     3181 gttttcctga atcgccgaag taattaacca gcaggtaaca catctgccag ccgtgtcgca
     3241 tttcttcgct gttgacgcgg gtcagcgctt tgcggtcgta ttctgtaggc gcggtgtcga
     3301 gcaggttctt ctgctgctct actgacgcga attccgtatc gccctgataa acgatcaaat
     3361 tcatcagcgc atcgcgaatc gcctgattcg gtacttgccc gattcgttgc catttcggct
     3421 ggcccttgaa gtcgccgaat tcgatctccg agctgtgaag ctcgcccagt ttcgtttcaa
     3481 atttataatc gcccagcagg gccgggtcat agccgatatc tttttgccag aaacggaagt
     3541 aatcaatcca gtcatcaaaa tttgcgattt tttgcatggt ttaatccttt cagaaaggag
     3601 cctgttcgaa cgatcgttcg aatttaatct aactcgcgcg atcctcaaaa tcaacccggt
     3661 cagaagggaa ttttttagca caacctcatt aatgatacat ctccatcaac tcgaccatgc
     3721 ccagctcccg catctgctcg aacaggtatc tcttaatttc ggcagcggta tccatttcga
     3781 gaactattcg ggccatcgat ttggccgcct cataggtgac cgatcgaatg gtttttttaa
     3841 ccaccggcac gaacagcgag cccatactga attcttctaa acccatgccg agcaggacca
     3901 gggcgtaaat cggatcgccc gccatctcgc cgcacattcc gactctttta ccgtctcttt
     3961 ttcccgcctg gatcgtggag taaagtgccg caagcacagc cggatgaagc ggctcataga
     4021 gatttgccac tttgcggttg ctgcgatcaa ccgcaaggat gtattggatc aaatcgttag
     4081 taccaatgct taagaaatcg acctcgcgca ggagtcgttc ggcaagttga accgccgccg
     4141 gaacttccac catgatcccc agctccatat tcaggtcgaa aggagtggac tcccgctgca
     4201 gctcatcttt ggcctcggcc aacaattcct tgaccttaag gacttcttcc agactcgaga
     4261 tcatcggtat cagaaggcgc acccggccca gtgtgcctgc gcgaaggatc gcgcgaagct
     4321 gagttttaaa gatctcttcg acatcaagcg agattcgaat tgatcgccaa ccgaggaaag
     4381 gattgggctc taccttggtg ctgcgcatat aggagggata cttgtcggcg cctatgtcca
     4441 aggtccggac cgttaccggt ttaccagcca tgccttcgac cacacgcttg taaagcgaat
     4501 actgctcctc ctcgctggga aaatcgcggt gggccaagaa cggaatctcg gttcgataaa
     4561 gccccactcc ctgggcgccg tggagatgag cgaaggcgat atcgctcaac aaaccgatgt
     4621 tcgcatacag cgaaactcta tgaccatcga tggtctcggc cggctcgtcc ttcagttctt
     4681 cgagctcgcg gttaagcgcg gcgtagtcac gctcgagccg atcgtattca cgggcaactt
     4741 cgggattcgg attgacgtaa agcacgccgg aattgccatc gacgatgacc gcgtctccct
     4801 gatgcacgat gtccatcagg ccctcgatcg cgaccacact gggaatctcg aaggacttcg
     4861 ccaaaatgga cgcgtgcgtc gtcaccccgc cggttgaaag gacgatgcca cggaagtggt
     4921 cgccttcaag catgctcaga tccgcggggg aaagatcttc ggccaccagc accgcatcgc
     4981 tgggcacgtc tatgtgctga tccggtgtcc cggcaaggtt ctctaacagt ctctgcgccg
     5041 cgtctttaac atccgtggtc cgctctctca gataatcgtc ctgaatttgc gatatcgagc
     5101 ttagatactg ctcgaagacg agccgcacgg catattcggc aacgtaattt tctttgcgga
     5161 tctgagcttc aatttgctga atgatggccg ggtcctcgag gattaagcgg tagacatcga
     5221 aaatagcgcc ctcctcgcgt gagatcagat tactcattct ctgcttgacg acgttgattt
     5281 gctcgattcc ccgctccacc gcgccgcgga atctttcgat ctcacgttgc gggttgcgag
     5341 cgcgtttttt ctcaatcgca gtgagttcca tccgcgggtt caagacgaag gccctgcccc
     5401 ggccaaatcc aggcgatgcc gcaagcccga ccagacggcc gcgccattta tgcgatgttt
     5461 tcgaggtctt gtcgcggcgg cgtccctcgt acgagcgaag cttgcgcagc gcatcgacca
     5521 tgcgcttctg atactccttg cgctcacgtt ccttgttgcg cagtgattcc aggagccgcg
     5581 cctggacaat gatacttgct gcctgagcag aaatcgtaat taacaaacgg atctcatcgc
     5641 gcgagaactg gcgccgccgc gaggtctgga cgacgagcac gccaagcggc gacttgttgt
     5701 tgaacagcgg cacgcctaag aaagagtgaa aacgttcctc gccggtctcg ggaaaatacc
     5761 tgtagcgcgg gtgggcgagc gcatccacca ccatcaccgg ctccatacgc tcgatcacca
     5821 gaccggttaa tccttcgccg atggccatcg acactttgcc gatggattcc ggatctaaac
     5881 ccatcgtggc acgcaatgta agacgtttct tttgcgggtc gaggatgtaa atcgaacaga
     5941 cctcggcctc catccggtcg cggacggttg tgacgatgtt ctgtaaggtg tcttggagat
     6001 catgagaatg ggtgatcagc gtgctgatat cttccaggat ggtcaggtgg gggtgtagtt
     6061 cgcggccggt tttttttagt tttgccatat tttcaaacgc tggagagact cctttataga
     6121 gcataaattt gcttttttcg ccagcgaacc ttaccagatg gtggccagaa tgcctatgtc
     6181 caatggccga gcaactaagg gaaatctgcc ggcgagtttg cgccgacatc ttcgctatca
     6241 gagatcccga ctaacgcggc gaataaccgg cggcttaatg gaagaactga tgccggtaga
     6301 aaagaaatcg agcgaatttt gcttagctat cgcaagcgaa agcgattgca gcacgggccc
     6361 ggcggcatga tatattacgg ccatgagtca aatcgatgga atcaaaggga tgcgccacat
     6421 agcgctcaaa gtcagagata tagccaaggc gaagagcttt tatcagaaca tgtttggcat
     6481 ggaggtggtt tgggagccgg atccggaaaa cgtatatctc tcatcgggct acgacaatat
     6541 tgctcttcac caggtagcgg ccggctttgc cgctacagca gcggagcagc acctcgacca
     6601 tttcggcttc atcgtagaaa gtatcgagcg ggttaaagag ctcgaagagc agttccgttc
     6661 cgccggcgtt accatagtcc acccatttaa aatccatcgc gatggcagcg cttccttcta
     6721 ttgcgccgat cccgacggca tcgtcatcca aatgctttat gaacctcagt tgagccagat
     6781 agcgccgaat tgacgacagt cgcttctcgc gtatgcggga gtgcaatctt caactcgcgt
     6841 gcttcgacga atcgttgcag agctgtaaac cacaatcagt tcccactcac ccggctcttc
     6901 aacatctcga gccggcacaa tgctggcgct tgtcatagac aatgccgcaa cgtatatgtt
     6961 aagtgatttt tttcactggc gaataatata aggagaagag atatggcgac aaaactcaag
     7021 ttcggattgc tcttacctca tttcggcgaa tatgcgtcgg ccgaaagctg tatcgaggga
     7081 tcgaagagag cggaagagta tggcttcgac tcggtttggg tcagggacca cctggttttt
     7141 gagccgcacg gtatggaggg aaacgataac acccatatcg agggactgct gatacttgcc
     7201 gcgatcgcgt ccgtgtgcaa aaaactcact ctcggcaccg gcacgatgat ttcgcaccgg
     7261 catccgattc atttggcgca ggccgtcgcg ggcctgagca cactctgcga cagcaatatt
     7321 atcatgggtc ttggtctcgg cacctttcct cacgaattcg aagcggcagg ttataagaaa
     7381 gtaactttgc aggatcgtgc caacatcgcc aaggccaacg cgctgctatg ccgccgcttc
     7441 tgggccggtg aaaagatcga acattataaa gatgactact acgaattcaa agacgtcgag
     7501 ctcagaccca cgccgagtaa aaacattccg atctggtatg gtggcggcac gccggcctcc
     7561 tgccgccgcg catcggatta ctgtgacggc tggatgcacg gacgtattcc cacggctact
     7621 tttaacaaaa tggtcaagta cctcgaagag cagtgcaagc tggcgggacg tccgatggta
     7681 accaccggcg cgatcccgat caccagcatc gataagaatc gcgatgttgc gctcggcaaa
     7741 gtcaacacca agggactgat caacgaagga aactctccga gcaaaaaaac ctgggttaaa
     7801 ccgaaatcag gcaccttctc tacggtggag gacatcgaag ggctgctaat ggccggaact
     7861 ccagccgata ttgcccggga taccaaacgc tacgaagaag ccggtttgaa tcatatcgtt
     7921 ttcgatctgc gattccgcta cgccgattgg tacgagcaga ttgattattt gggccaggaa
     7981 gttctaccca aggtgcgggc gtaacgtagg tttcgggtgc gaggtttggg tctcggataa
     8041 tgaaagtcca gatttggttt gaggtcgttc aaaaaggttt agatgcgagg cgcgcgagaa
     8101 atcgatgagc ggagacgtac tctagtacgt tgagtaacgc gatcgagcgc aacgaagcag
     8161 atgagccttt ttcaacggcc tgtcacagga gtttgatatg cgtacagaga aggtgaattt
     8221 tttcagcgaa ggcatcaagc tcgccggcgt gctctacttg ccggattcgg ataaagacaa
     8281 gccgtttgcc ggtgtcgtgc aaggccccgg ttttctcggt ctcaaagatg ccaagcacta
     8341 catcatgatg ttcgagaagc tgtgcgccgc gggctacgcc tgcttatgct tcgattaccg
     8401 cggctggggt gacagcgaag gtaatgatcg cggctgggtc gtgccgaaat ggcaagcgga
     8461 agacatccgc aacgctatca cctttatgca aacgcgagcc gagatcgatc ccgatcggat
     8521 cgcgacttac ggctccggcg gcaccggcgg cggcaacgcc gtatatgttg ccgccattga
     8581 tcaacgagtc aagtgctgcg tcagctattt gggtgtcagc aacgggcggg actggctcca
     8641 ttcaatgcgg cgcgagtatg aatggatcga ttatctcaag agcatcgaag aggaccggaa
     8701 gaagcgggtg accaccggct ccggcgccat ggtcagcgcc cgtgaagggg gcatcatggt
     8761 gcaaacccct gagcgccaga caacagctat caaaaaagac gttgccgata agattcctga
     8821 cgccatgccg ctcgcctgcg ccgaagcgat tctggaatac agccccatcg atgtggtgga
     8881 taagatttcg ccgcgcggcg tattgttcat cgccgtggaa ggcgatgcgg tcacgccgga
     8941 gcagcagtcc ttcgatttgt atgaaaaggc ccgcgagccg aaaaaactcg tgctctacaa
     9001 gaagaccacc cattacggca tttacaacga ttacttcgat gacgtggccg ccaacatcgt
     9061 cgactggtat aaccgtcacc tgaagtacaa caaagtcgat atcactgaaa accattagga
     9121 gcagttcatg cacaagattt cgtttggcgt gcgggtgcca aactcgggcc cgctttccag
     9181 cgtggagaat atcgtcaaag ccaccaaaac ggcagaagag ctgggctttg acgccatctg
     9241 ggtccacgac cacgtggtct ggagctcgga aatgcatcgc catcacattt cctccggcgc
     9301 tgctgaggcg ttgaccgaca ctcaggaggc gaacttcttt gaagcgacca caatcctctc
     9361 ctatctggcg gcggaaacca agacgatcac gctcggcgtt gcctgcctgg taatgccctg
     9421 ccgcaatccg atttatgcgg caaagcagta ttcaacattg gatcacctgg ccaagggccg
     9481 tttgttggtc ggcgtcggtc tcggctcaaa agccactcgt gaatccgatg agttcggcgt
     9541 ttttggtgtg ccttacgagc ggcgcggcga tcgcaccgat gagtatatcg aagcaatgaa
     9601 agcggtttgg acacaaccct tagcatccta caagggtgaa tttatcgaat ttaaagatgc
     9661 cgaggtttat cccaagccag tgcaaaaacc ccatccaccg gtttgggtcg gcggctggat
     9721 gaagctcgcc gccaagcgcg ccgggaaata cggcgaagga tggattcccg gctggctctc
     9781 gcccaaagaa atgaaagtcg ggtgcgacat tctggcgcag acggcaaagg acaatggccg
     9841 tgatccgaaa aagataacta ttgcggttga aaagctcgcg accatcgcca agacccgcga
     9901 cgaaggcttg cagcttgcat tgccgaccct gaaaaccagc agcgaaagtt acgagcgcga
     9961 tatcgacagc atgcagtttg ccctggatcg ccacatcttt ggttccgttg atgacgtgcg
    10021 ccgtcgtgtc gatgagttca ttgagaacgg cgttcagcat ttcgagctta agattatcta
    10081 tcccaccatg gacagcttgg aacgtcagat gaccctgtgg gccgagtcgg ttttgccgca
    10141 ttacgagtga tcgaatgaga aatgtcgctt tgaagtttca tctcaatggg aggccggttg
    10201 aactcgagtg cgaggccgaa gaaactctcg ccgaaattct ccgtaaccag ctcaatttaa
    10261 acggcaccaa agtctcttgt gaggttcaag tctgcggcgc ttgtacggtt ctcgtagatg
    10321 acctgccggt aagcgcctgc acgtatctgg cgtacgaagc acacggtaag agcgtgacga
    10381 cgattgaagg cttggaaaaa cctgacggca ccctccaccc gatccagcag gcctttatcg
    10441 acgattttgc tttccaatgc ggcttttgca cgcctggcat gattatgtcg gccaaagcgc
    10501 tgctcgatga aaacccgaca ccgagccgcg aggaaatcat tcatcatatg gacggaaata
    10561 tctgccgctg caccggatat gtgccgatcg ttgccgccat tgaacgtgcc gctcaagcga
    10621 tgagcaggga ttcgaaaaag tagcgcagcc agaagtagcg caaaaaaccg acctatgtgt
    10681 caatcattca ctcattgaac gtgccgctca aacgatgagc agggattcga agaagtagcg
    10741 cagaaccatt gaacccgccg cccaagtgat gagcaactcg aagaactgac cgcagaaaag
    10801 aagccggttt ctatgtcgtt aatcattcct catagaacgt gccgctcagg agatgaacca
    10861 ggagtcaaaa gtgagcgcag agaagaaaac tgatttttat gtcgtcaacc attcagtgcc
    10921 gcggcgcgac ggccgcgtta aagtaaccgg aaaggcccag tatgtggccg acttgaagct
    10981 gatcggcatg gcctacgcca aggtgctgcg cagcccctat gcgcacgcca aaatcatttc
    11041 cattgataca tcgcgcgctg ccgcgcggcc cggagtattc tgcgtagtca ctggcaatga
    11101 tctcgaggga ctgaaccctt attacggcca tgcggtcaag gatcatccgc tgttggcgat
    11161 tgataaagtc cgttatgccg gtgagccggt ggcggctgtg gtcgccatcg atgaacgcac
    11221 cgctttcgaa gcgctcgaat tcatcgatgt caaataccag gagctcaagg cggtcttcac
    11281 gccgcaagaa gcgatggcca aggatgctcc actgctgcac gaaagacagt ttgaggccgg
    11341 cgcgctgcgt ggtttcgaag gtgaagtgac cgcaggcaag ggaagcaaca tctgccagaa
    11401 acacgaagta agatggggca acgtcgagga ggctttcaag aacgctgccg ctgtcgtcga
    11461 agcggactat tactttccga tgacctatgc ctacgcgatg gaagcttacg tcgctgttgc
    11521 cgatgttacc gatcaagggg ttactattta ctcctcggcg cagcatccgt tcatggtgcg
    11581 acacgacctc aaaactgtgt tcaatctacc ggtcagcaaa gtgcgtttaa tcgtgccgtt
    11641 tgtcggcggc ggctacggca gcaagtcgta tacgaaaatc gaaccgttgg tagcggcgtg
    11701 ttcatggaaa gccaaacgac cggtgaagct gcagctgacg attgaagaag cgatgctcac
    11761 tacgcgcagc gatgacgcct atacttggat gcgcacggct gtggatgaga acggcaaaat
    11821 catcgcccga caagcaaaaa tttacatgaa caccggcgcc tatgcggaaa acagccctct
    11881 tgtggtcgag aaatcgagca accgctgcgt tggtccttac gcgattccca atgtcttgat
    11941 cgagaattgt tcgctttaca ccaacaccgt gccggcgagt tcctatcgcg ggttcggctg
    12001 cgcccaggtg accttgcccg gcgaatcgca aatcgacgag ctcgccgcca aaatcggtaa
    12061 agacccttac gagttccgcc tggccaatgc cgccaaaccc ggtgaggaat ttttcccggg
    12121 aatgcgcccc ttcgatggga ccttaaaaga agatctcgac acggccgcaa acgcgattgg
    12181 ctggaaggat cctttgccca gtggtcatgg ccgcggcgtt gcctgctccg gcagcgatgc
    12241 cggagcctac ccgctcactt cgacggcggt acgggtgcat gccgatggtt cggtcacgat
    12301 catgaccggt agtaccgagc tcggccaggg aagccacacc gtcattccgc aaatcgcggc
    12361 ggaagagttg ggtgtcgatt tcgacaaggt caacgttgtt tcatccgaca ccgcgatcac
    12421 cccttacgat cgatccaccg gcgcgagccg caccaccaca ttgatgggca gcgccgttct
    12481 ggaagccagc cgcgaagcga tccagcagat ggtggtgatg gcggccgacg tcttgaaagt
    12541 gaaagtcgaa gacatccagg tagttcgcgg cggcgtgcgc tgcggtgaag cgcagctcaa
    12601 ttggtcggaa gtgatcagca agttttacgc gcttcccgac ggcgaggtca tcggccgttc
    12661 ctatattagg aagtcgggca agttcgcggc tctgccggta ttctgggaag ctgctgcgac
    12721 cggtgtcgaa gtttcggttg accaagaaac cggcaaaatc accatggaga aactggcgac
    12781 ggtcggcgat gttggcttgg cgatcaatcc ggccttggcg gaagggcagg ttgtcggcgc
    12841 cgcgactatg ggtatgggca tcggtttgtt
//
LOCUS       DASUTG010000315         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1086908, whole genome shotgun sequence.
ACCESSION   DASUTG010000315 DASUTG010000000
VERSION     DASUTG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086908"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF089_07240"
     CDS             complement(<1..265)
                     /locus_tag="VF089_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6173794.1"
                     /translation="MAAILALVMAMFWISVQKGAVGTPAAIYTLGNYFEVFSDPFVLR
                     VAANTIIFTITATTVALLFGLPIAWLAERSDLPGKTLIYAIMTL"
     gene            complement(571..1674)
                     /locus_tag="VF089_07245"
     CDS             complement(571..1674)
                     /locus_tag="VF089_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6173795.1"
                     /translation="MIVHKSTPRLAIWALTVMMTLASIAAVAQAETLAQIIEGAKKEG
                     MLRGQWGQNSFGGSEGFKEILAGMNKKYKLDLKGQYTPGPDMQRLMLRIIQENAAGQP
                     ASTDVYLGNAQAMFDGLKSNLLKPMTYNSFIEHKPKPEGKFNAIAADGTHVAVATAVV
                     GIQYNTDLVKGADIPRRLEDVLNPKWKGKIASTPYAAGMREFATPDFLGREKMIEFTK
                     KLSKQIGGLMRCGEAERITSGEFLMLAFTCGGNDVNVLKRAGAPLGHTVIEEGTVLHM
                     RYAAVPKNSRSPNAGALLINYLMSPEGQELLWKHDGFDLHLFPGSQTKRELETVLAAK
                     GKVIISSPEWLASFKNYAETQKELEAILRDAGK"
     gene            complement(1715..2791)
                     /locus_tag="VF089_07250"
     CDS             complement(1715..2791)
                     /locus_tag="VF089_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173796.1"
                     /translation="MISIRRLQKRFDVSDGVIAAIRSLDLEIAEGELFVIVGASGSGK
                     TTLLRCIAGLETPDSGEIRIGDRVVSSDNPPTWIPPQQRKLGMVFQSYAVWPHLSVCE
                     NIALPLAEGSQRIPRREVAQRVNEALRLVQLEEQADRPATLLSGGQQQRVALARAIAV
                     NSRIVLMDEPLSNLDARLREEVRGKIRELAKQLGSTMLYVTHDQIEAMAIADRIALMQ
                     AGELLQVGAPLEIYHKPNCADVAEFFGSVNWLDAQVVESGVADSAIGKLKIESSSQIG
                     DKVLLGFRPECLSLSGPSAQADGNCFRAKLGSRTFLGDQFVYTAVVNDRQLFGKSRAV
                     PEASSGATFCYVSPSDIMVFPSRV"
     gene            complement(2965..>3343)
                     /locus_tag="VF089_07255"
     CDS             complement(2965..>3343)
                     /locus_tag="VF089_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173797.1"
                     /translation="GMVFFRNSTDHHTVALMQAKENTPLPKRGQLGFDHFALEVATVS
                     ELFKIRDFLKSKGVTITYEGRRGPGGNPGVEFLDPDGYQIEIYASMDQIGEDGKSRPA
                     SEWRRATTLEDAVANPLPGAEYR"
BASE COUNT          734 a          927 c          915 g          767 t
ORIGIN      
        1 cgagtgtcat gatcgcgtag atcagcgtct tgcctggaag atcgcttcgt tctgctaacc
       61 aggcgatcgg caacccgaat agcagcgcga ccgtagttgc ggttatggtg aagattatcg
      121 tattggcggc gacgcgcagc acgaacggat cgctgaaaac ctcgaagtaa ttgcccagcg
      181 tgtagatcgc agcgggcgtg ccgacggcgc ctttctggac gctgatccag aacatcgcca
      241 tcaccagcgc caggatcgcc gccatcggca caagcgcgac cgacaacaga gccgaagaag
      301 tttgacctct ttggtcaaga catgcgccga gtcgtgataa gagctgtttc atggcgttgg
      361 gctaaggcca aactctcttg ttaaacgatc attcgctgac agcgccaaga tgatcctgcg
      421 cctttaggag gaaggatctg aaccggagcc caatggacaa aacaaaagca gatcctcgca
      481 actcaggacc acaggtgaaa cttgccttgc taaaattctt agacccgatc tcgacgattc
      541 tgctgcgttc atggacgaaa ttccggctac ctacttgccg gcatcgcgta gaatcgcttc
      601 cagctccttc tgtgtttccg catagttctt aaaagaggcg agccactcgg gcgaactgat
      661 gatgacctta cctttggcgg ccaggactgt ttcgagctcc cttttagtct gggacccagg
      721 aaatagatgc aggtcaaaac catcatgctt ccataagagt tcttgccctt cggggctcat
      781 aagataatta atcagcagcg cgccggcgtt aggggaacgg gaatttttcg gcacggcggc
      841 gtagcgcatg tgcaggacgg ttccctcctc aatgaccgtg tgacccaatg gcgcgccggc
      901 gcgcttcaag acattgacgt cgttgccgcc gcaggtgaag gcgagcatga ggaattcacc
      961 gctggtaatc cgctctgcct cgccgcatcg catcaatccg ccaatctgct tggataattt
     1021 tttggtgaac tcgatcattt tctcgcgccc cagaaaatcg ggcgtggcaa attcccgcat
     1081 tccggcagca tagggcgttg aagcgatctt gcctttccac ttcgggttga gcacgtcctc
     1141 aagacgccgg ggaatgtctg cgcccttgac gagatcggta ttgtattgga tgccaaccac
     1201 cgcagtggca acggccacat gggtgccatc cgcggctata gcgttaaact tgccctcggg
     1261 tttcggcttg tgttcgatga agctgttata ggtcatcggt ttcagcaggt tcgacttcaa
     1321 gccatcgaac attgcctgag cgttgcccag gtaaacgtcg gtgctggccg gttggccggc
     1381 ggcgttctcc tggataatac gcagcatcaa ccgctgcatg tcgggcccgg gcgtgtactg
     1441 cccttttaga tcgagcttgt actttttgtt catgcccgcg agaatttctt tgaacccctc
     1501 actgccgcca aagctatttt gaccccattg accgcgcaac ataccttctt tctttgcacc
     1561 ctcgatgatc tgggccagag tctcggcctg cgctaccgca gcaatgctag ctagcgtcat
     1621 catcactgtt agagcccaaa ttgcgagccg gggcgttgat ttgtgcacga tcatatagcc
     1681 tccttcggtt tacatggtca caagatcggt ggagttatac tcgggacggg aacaccatga
     1741 tatcggacgg actcacataa cagaatgtcg caccactgga tgcttccggg actgcgcggc
     1801 tctttccgaa cagttgccgg tcgttgacca ctgccgtgta gacaaattga tctccaagaa
     1861 aagttctcga accgagcttg gcgcggaagc agttgccatc agcttgcgcg ctggggccgg
     1921 acaaggaaag acactcggga cgaaacccca gcaacacctt atcgccgatt tgcgatgaac
     1981 tttcgatctt caattttccg atggcggaat cggctacacc ggattcgacc acttgagcgt
     2041 ccaaccagtt aaccgagccg aagaactctg cgacatcggc gcagttgggc ttgtgataaa
     2101 tttccagggg cgcgcccacc tggagaagtt cgccggcttg catcagagca atcctatcag
     2161 caatcgccat agcctcaatc tgatcgtggg tgacatagag catcgtcgat ccgagctgct
     2221 ttgccagctc gcggattttg ccgcgaactt cttctcgcag ccgcgcatcc aagttactta
     2281 gcggctcgtc catcaggaca atgcgcgaat tcaccgcgat tgcgcgcgcc aatgcgaccc
     2341 gctgttgctg cccgccgctg agcaaagtcg caggtcgatc ggcttgttcc tccaattgca
     2401 ccagccgtag agcttcattt acacgttgag ccacctcccg tcgaggaatc cgttgcgatc
     2461 cctcagccaa cggtagggca atgttctcgc aaactgagag gtgaggccag actgcgtagg
     2521 attgaaaaac catgcccagc ttgcgctgct gcggggggat ccaggttgga ggattatcag
     2581 aggagacgac tctgtcgccg atgcggattt cccctgaatc tggggtttcg aggccggcaa
     2641 tgcagcgcag cagagtggtt ttccccgagc cgctggcgcc gacgatgacg aaaagttcac
     2701 cctcagctat ctcaagatca agggacctga tggctgcgat gacgccgtcg ctaacgtcga
     2761 aacgtttctg cagacggcgg attgaaatca tagaatcgag ctagttaatg cgttgcgttt
     2821 gtctcggcga cgattgtgaa ctgtcgtgct tgtcgtttca tgcgcgggat gatcagcgca
     2881 ggtcttaaaa tacctccgga gataaccaac gtatcgttgg ccgaaacaag ttcggccaga
     2941 caagacaatc tttagaaagt aaactcaacg gtattcggca ccgggtaatg gattggcaac
     3001 cgcgtcttcc agggtagttg cgcggcgcca ctcgcttgcc ggacggcttt ttccgtcttc
     3061 gccgatctgg tccatagaag cataaatctc gatttggtaa ccgtcggggt caagaaattc
     3121 gaccccggga tttccccccg gtccgcggcg gccttcgtat gtgatggtca cacctttgga
     3181 ttttagaaaa tcgcgaattt tgaagagctc cgaaacggtg gcgacctcga gggcaaaatg
     3241 atcgaacccg agctggcccc gtttcggcag cggcgtgttt tccttggctt gcattaatgc
     3301 aaccgtatgg tggtcggtcg aattgcgaaa gaagaccatg ccg
//
LOCUS       DASUTG010000316         4514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_790910, whole genome shotgun sequence.
ACCESSION   DASUTG010000316 DASUTG010000000
VERSION     DASUTG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790910"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VF089_07260"
     CDS             complement(<1..384)
                     /locus_tag="VF089_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6173798.1"
                     /translation="MVEMFSALSSGLVQVFNWSTFSLMMIGIGVGFVVGILPGLGGPT
                     AMALMLPFVFKMNAVEAFAFLLGMTAVTATTGDITSVLFGVPGEPTTASTIVDGHSMA
                     RNGEAGRALGAVLMSSLVGAIFAAIA"
     gene            complement(452..1477)
                     /locus_tag="VF089_07265"
     CDS             complement(452..1477)
                     /locus_tag="VF089_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173799.1"
                     /translation="MWQVFCFFTAAILGLFSAQPGAAAEDFYKDKTIRIVVGFSAGGG
                     FDTYARAIARHMGKHIPGQPSIVVENMTGAGSLIAANHVYKVAKPDGLTIGHFIGGLF
                     LGQVLGQKGIEFDARKFEFIGAPITDHVVCALTKASGITSVEGWMASKTPIKMGGIAP
                     GTSTPDNATRILKEALGLPIQIVSGYKGTADVRLAAESGEIAGGCWGWDSVSVTWQKA
                     LASGEAVVVLQANRRNHPDLPKVPQAIKLAKTEEGRKMIEVGIHGDSDIVRTYTLPPG
                     TPKDRVQILRKAFDATLKDSEFLADAKKSKLNVDPVPVEEVEKVIAGLFNLDQALLGK
                     LKQILYN"
     gene            1717..3207
                     /locus_tag="VF089_07270"
     CDS             1717..3207
                     /locus_tag="VF089_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6173800.1"
                     /translation="MSEVFKNYIDGKWLPAQAGQVIENRNPANRNDLVGLFPASDQND
                     VGTAVAAAKKAFASWRLVPAPKRGELLYRVGELLRAHKEEIARIMTREMGKVLKETRG
                     DVQEGIDTAFYVAGEGRRLFGETTPSELPDKFAMSVRAPVGVCALITPWNFPMAIPTW
                     KMFPALICGNTVVLKPAEDTPYTAVKLFEILQRAGVPPGVVNLVQGRGEEVGAALVRH
                     PDVQLVSFTGSAAVGREVAAACGQSLKRVSLELGGKNAQIVMEDADLGLAVEGALWGA
                     FGTTGQRCTATSRLILHHDIKTEFIDRLVARVEKIKIGDGLDESVEIGPLINQEARDK
                     VQAYMEIGRQEGARVLIGGSAYDSGACTNGFFFQPTILDRVTPNMRVAQEEIFGPVLS
                     VIEVPDFDAAIAVLNGTRYGLSSSIYTSDVNRAFRAMRDIEAGITYINGPTIGAEVHL
                     PFGGVKDTGNGHREAGTTVYDIFSEWKSVYVDYSGRLQKAQIDNAE"
     gene            3265..>4514
                     /locus_tag="VF089_07275"
     CDS             3265..>4514
                     /locus_tag="VF089_07275"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX6173801.1"
                     /translation="MKAPLIKTKLPGPKAQAIIEKDRQYTAPAYGRVYPLVVKQAQGM
                     VVEDVDENLFLDFMAGIAVANTGHSHPRVVQAIEEQARRFLHICGSDFYYQPMMELAE
                     KLSQLTPGPGTKKVFFTNSGTETVEAAFKLARYATKRQHVIAFHGAFHGRTMGALSLT
                     ASRSSHRAQFSPLVPGVHHVPYAFCHRCPYHLLYGSCEMGCVSYLQKILFRYEVAPQE
                     VAAIFVEPIQGEGGYIVPPPEYLGMLQDLCRRHGILLVADEIQSGFGRTGKMFACEHW
                     GIEPDILCAGKGIASGMPLGAMIARSEISTWTPGTHGSTFGGNPVACAAALASIAVIE
                     AGLLLNATEVGAYLIDKLNQLKNKHAVIGDVRGLGLMIGVEFVRADGGGTPDAAFRDK
                     VMKNCFERGLLLLGCGESTLRFCPP"
BASE COUNT          971 a         1223 c         1286 g         1034 t
ORIGIN      
        1 agctatggcg gcgaaaatgg cgccgaccag cgaactcatc agcaccgctc ccagcgcccg
       61 accggcctca ccgttgcgcg ccatcgagtg gccgtcgaca atggtcgaag ccgtcgtcgg
      121 ctcgccaggg acgccaaata agaccgacgt gatatccccg gttgtggcgg taaccgccgt
      181 catacccaga agaaaggcaa acgcttctac cgcattcatt ttgaaaacaa acggtaacat
      241 caaagccatc gcggtaggcc cgcccaagcc cgggagaata ccgacgacga agcctacgcc
      301 gatgccgatc atcatcaaac tgaaggtaga ccagttgaaa acttgaacga gtccggagct
      361 cagcgcgctg aacatttcca ccattaggct acctcgacgc gatacagcga atcattattc
      421 tggttagtta atgatgatca atcccctgtt tttagttgta aagaatctgt ttcagtttgc
      481 cgagcagcgc ctgatcgaga ttgaaaaggc cggcgataac tttctccacc tcttcgaccg
      541 gcacaggatc tacattcaac tttgacttct ttgcatctgc gaggaattca gaatctttta
      601 gtgtcgcgtc gaatgcttta cgcagaatct gaactcgatc cttcggagtc cctggcggta
      661 gcgtataggt gcgaacgata tcgctgtcgc cgtggattcc cacttcaatc atcttgcggc
      721 cctcttctgt tttcgccagc ttgattgctt gcggcacttt aggaagatct ggatggttac
      781 gacggttcgc ctgaaggact accacggctt cgccgctggc cagtgccttc tgccacgtca
      841 cgctgaccga atcccagccc cagcagccgc cggctatttc gccgctctcc gcagccagtc
      901 ttacatcggc ggtgcctttg tatcccgata cgatttgaat tggcagtcca agcgcttcct
      961 tcaagatccg cgtcgcgttg tccggggtcg aggtgccggg ggcaatccct cccattttta
     1021 tcggggtctt ggatgccatc catccttcga cactggtgat gccgctcgct ttggtcaacg
     1081 cgcacaccac gtgatccgta attggcgcgc cgatgaattc gaatttacgg gcatcaaatt
     1141 ctatgccctt ctgtcccaac acctggccca agaacaggcc gccaatgaag tgaccgatgg
     1201 taaggccgtc tggtttggca actttgtaga cgtggttcgc tgcgatcagg ctgccggcgc
     1261 cggtcatgtt ctcgacgacg atcgatggtt gcccagggat atgtttgccc atatgccggg
     1321 cgatggcgcg ggcgtatgta tcaaatcctc cgcctgcgga aaacccgaca acgattctga
     1381 tagttttgtc tttgtaaaaa tcttccgcgg cggcgccggg ttgggccgaa aacaatccga
     1441 ggatcgctgc cgtgaagaaa caaaaaactt gccacataga agttctcctt ctgctcgcga
     1501 agtttgcccg ctcgcccatc cgcttctccc ggccggcgag ttgccagaag ctaactgatt
     1561 gatagttaaa aatcaacgaa ttgcacgagt ttaggaaggc ttatcgaatt tctgacgttg
     1621 tgttcgcgct gggccggttc tagaatggtc ttgctgttgt cggcagtgat cacccctggc
     1681 aagacgagat tcaaacataa gaccgggaga acagttatgt ctgaagtatt taaaaactat
     1741 atcgatggca aatggctgcc ggcgcaagcc ggacaggtta ttgaaaatcg caacccggct
     1801 aaccgcaacg accttgtggg tcttttccca gcctcggatc agaatgacgt cggtaccgca
     1861 gtagcggcgg ccaagaaggc cttcgccagt tggcgccttg tgcctgcgcc caaacgcgga
     1921 gaactgctct atcgcgtcgg cgaacttctg agggcgcata aagaggagat cgctcggatc
     1981 atgacacgag agatgggcaa ggtcttgaaa gaaactcgcg gcgacgtgca agaaggcatc
     2041 gacacggctt tttatgtggc gggtgaaggc cggcggctgt ttggtgaaac gacaccctcg
     2101 gaactgccgg ataagttcgc catgtcggtg cgcgcgccgg tcggcgtttg cgcattgatc
     2161 acgccgtgga attttcctat ggcgattcca acctggaaaa tgtttccggc gttaatctgt
     2221 ggcaatacgg tggtgctcaa gccggccgag gatacgccgt ataccgccgt taagcttttt
     2281 gagattctcc aacgggctgg cgtgcctccc ggcgtcgtta atttggttca ggggcgcggc
     2341 gaagaagtgg gtgccgcgct ggtgcgccat ccggacgtgc agttggtttc cttcaccggt
     2401 tccgccgctg tggggcggga ggttgctgct gcctgcggcc agtcgctcaa gcgtgtctcg
     2461 cttgagctcg gggggaaaaa cgcccagatc gttatggagg atgccgattt aggtcttgcc
     2521 gtcgagggcg cgctgtgggg cgcatttggt acaaccggac agcgctgtac cgccaccagc
     2581 cggctcattt tgcatcatga tattaaaact gaattcattg ataggctggt ggcgcgagtc
     2641 gagaagatca agatcggcga cggtttggat gaaagcgtcg aaatcgggcc gctgatcaat
     2701 caggaagcgc gcgacaaggt tcaagcctat atggaaatcg gccggcaaga aggcgcgcgc
     2761 gtgctgatcg gcggctcggc ctatgacagc ggcgcatgca ccaatggttt tttcttccag
     2821 ccgacgatat tggatcgagt cacgccgaat atgcgcgtgg cccaagagga aatattcgga
     2881 cctgtgcttt cggttatcga agtgccggac tttgatgcgg ccatcgccgt gctaaacggc
     2941 acgcgctatg ggctttccag ttcgatctac acctctgacg tcaaccgcgc ctttcgcgcc
     3001 atgcgcgata tcgaggcagg catcacctac atcaatgggc ccaccattgg cgcggaagtg
     3061 catctgccgt tcggcggcgt taaggatacc ggcaacggtc atcgtgaagc tgggaccacc
     3121 gtctatgata tcttcagtga atggaaatcg gtttatgtag attactctgg caggttgcag
     3181 aaagcgcaga tcgataatgc ggaatgagtt tcacagggcc cgagattgcc tgctattcaa
     3241 aatgcgacag ccatggagaa ctgcatgaaa gcacctttaa tcaagacgaa actcccggga
     3301 cctaaagcgc aggcaatcat cgaaaaggat cgccagtata ctgcgccggc ttatggccgt
     3361 gtttatccgc tggtggtgaa gcaagctcaa gggatggtgg ttgaagatgt cgatgagaat
     3421 ctttttctcg attttatggc cggcattgcc gttgcaaata ccggccactc tcacccgcgc
     3481 gtagttcaag ccatcgaaga gcaggcgcgc cgattcttgc atatctgcgg cagcgacttc
     3541 tattaccagc cgatgatgga gctggcggag aagcttagcc aattgacgcc tggacccgga
     3601 accaagaaag ttttcttcac caactcgggc actgaaaccg tggaggccgc cttcaagctg
     3661 gcgcgctacg ctactaaaag gcagcacgtg atcgcctttc atggcgcttt tcacggccgc
     3721 actatgggtg ccctatcctt aaccgccagt cgctcatcgc accgggcgca gttttctcct
     3781 ctggtgccgg gagttcatca cgttccttat gccttctgcc atcgatgtcc ctatcatctc
     3841 ctgtatggtt cctgtgaaat gggatgcgtg agttatctgc aaaaaatact gtttcgttac
     3901 gaagtggcgc cacaggaagt cgcggctatt tttgtcgagc cgattcaagg cgagggcggt
     3961 tatatcgtcc cgccccccga gtacctggga atgcttcaag atctctgtcg ccgtcatggc
     4021 attttactcg ttgctgatga gatccagtca ggcttcggcc gaactggaaa gatgtttgcc
     4081 tgtgaacact ggggaatcga gcctgatatt ctctgcgcgg gcaagggcat tgcgagcgga
     4141 atgcccttgg gggcgatgat cgctcggagc gagatcagca cgtggacccc gggcactcat
     4201 ggttccacat tcggcggcaa tccagtggcg tgcgccgcgg cgctcgcgag catcgctgta
     4261 atcgaagcgg gactcctgct taacgccacg gaggtggggg cgtatttaat cgacaagctc
     4321 aatcagctaa agaacaaaca tgcagttatc ggcgacgtgc gcggtttggg attaatgatt
     4381 ggggtggaat tcgtccgcgc cgatggcggt ggtacgcccg acgccgcgtt ccgcgataag
     4441 gtgatgaaga attgtttcga aagaggacta ctcctgcttg gttgcggcga gagcaccctg
     4501 cgcttttgtc cgcc
//
LOCUS       DASUTG010000317         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1829708, whole genome shotgun sequence.
ACCESSION   DASUTG010000317 DASUTG010000000
VERSION     DASUTG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829708"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF089_07280"
     CDS             complement(<1..390)
                     /locus_tag="VF089_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173802.1"
                     /translation="MSLSSRVKVAISEAKIFYGWVIVGVGFLAHIASAFSISSTLSVF
                     LKPLSQDLGISRGVFSLIRSGEILVGALAAPLVGSLLDRHGGRWLIAAGGLVSGAGFL
                     LLGQARDFWQFAIVRWLLVSPGDTLMGS"
     gene            591..1613
                     /locus_tag="VF089_07285"
     CDS             591..1613
                     /locus_tag="VF089_07285"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6173803.1"
                     /translation="MSISNMPHDCDMRQCGDRALQMANKENSGWVVLFTATLWLIPMT
                     APVHAHIADGESVLTDWHWRWDIISVLFVFAALYIRGWLRLRAMGGEAKVSQVVFYVV
                     ALVAIGGALLSPLDDLASYLLIAHMVQHELLMMLAPPFILLANPVPVLLWSLGRNPRL
                     QAGHLLAHHSPMRRVRDRLGSMPVAWSLYVINLWAWHYPALYDAALRVSWIHDLEHVL
                     FFLTALIFWWPVIQPGSRPAPIQHGLRILYLFFAATQDTILAGLIALSREVLYPHYET
                     ALRLWDLTPREDQIGGGIVMFAVGSTTYAVAILILVNALLGEGRRKQSTKRTLSDGAE
                     KVEGRI"
     gene            complement(1767..2096)
                     /locus_tag="VF089_07290"
     CDS             complement(1767..2096)
                     /locus_tag="VF089_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173804.1"
                     /translation="MNHEHPSSDELEHYAGGEIHARHGIVNRWLLGLYAVLFLWSIHY
                     LIGPFDGLRPKFEFWGWGGLGAGLSSKGAQEGVEGLQVIGVIALSIVFISIIGFFGWV
                     VMLARKK"
     gene            complement(2086..2241)
                     /gene="ccoS"
                     /locus_tag="VF089_07295"
     CDS             complement(2086..2241)
                     /gene="ccoS"
                     /locus_tag="VF089_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX6173805.1"
                     /translation="MLELITLIEFIVAFLMGLAACCFLFWGILDGAFDNLEDIKHRIL
                     EIEKDEP"
     gene            complement(2321..>3752)
                     /locus_tag="VF089_07300"
     CDS             complement(2321..>3752)
                     /locus_tag="VF089_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEX6173806.1"
                     /translation="WYCHSQYIRPVTGEDQRWGPVSQAGEYAFDVPHLFSTRRIGPDL
                     TRVGLKYSDHWHFAHHWNPRLVVPDSNMPSFPWLYEQITVELVEKDGKLAFKDDSSLR
                     KIFTFKQGTEIQILPNIDGLAFARERDGVPVLNFDPLPDPPKTSTITLVTPTREAEGL
                     VAYIQKLGMNRGQWRDLFEPQRVFVSQFSMPESEDWIAHGKEVYLRRCVGCHGEKGDG
                     NGPAATFLNPRPRDFREAVFKFRVTPSGALPTEGDLYRTITRGIRGTAMPPWHEIPEK
                     QRLAVILFIKTFSPAWKDKNLKEAPVALPDPPAPSEELITQGEKLYINEGRCWNCHGG
                     PGIKDNQPFTYPWGRGPSAPTLKDDWGFPLLASDFTTGIFKSGSDVTDIFRTMSTGLN
                     GAAMPSFATNLTDEQRWAISYYVLSLSAYTDPLHRKKLQIPEQVKAALDDPNLKTPTW
                     RAAFDPSQAETTVAGEKSKNYLRIAE"
BASE COUNT          823 a         1014 c         1028 g          887 t
ORIGIN      
        1 cgagcccatg agcgtgtcgc ccggactgac aaggagccag cgaacgatgg caaactgcca
       61 aaaatcccgt gcctgaccca gcagtaaaaa acccgctccc gagaccaatc cacctgcggc
      121 tatcagccac cttccgccat gacgatccaa tagagagccg accagtggcg ctgccagtgc
      181 gcctacgagt atttctccag atcggatcaa ggagaaaacc ccgcgcgaaa tacctaagtc
      241 ctgcgacagt ggtttaagaa agacgctcag ggtgctcgat atcgaaaatg cagaagcgat
      301 gtgcgcgagg aagccgacgc cgacaatcac ccagccataa aagattttgg cctcactgat
      361 tgcgaccttc acgcggctag agagtgacac cgcctgcttg tatcaaggtt tggagcgaaa
      421 ctccaagatg atttcccgtt ggcgctgacc ctggcatgtc atcacgggcg caatattcac
      481 tgtcgcgatc aacgcgcaaa agttcgaacg cttcaatgtg agagaatttg attaaaagcc
      541 ctttatcgcg atgaaactcc gccagttata gttggggcgg aacgacaaac gtgtcaattt
      601 ctaacatgcc acacgattgt gatatgcgtc agtgcggtga tcgggcgctg cagatggcca
      661 acaaagaaaa cagcggctgg gtggtccttt tcacggcaac gttatggttg ataccaatga
      721 ccgcaccggt gcacgcgcat atcgccgatg gcgagagcgt gctgacggat tggcattggc
      781 gttgggatat catttcggtc ttgtttgttt ttgcagcgct ttatattcgc ggctggctgc
      841 gcctgagagc catgggtgga gaggcaaagg tttcccaagt ggtgttctat gtggttgctt
      901 tggttgccat cggcggcgcg ctgttgtcac ccctcgacga cctggcctct tacctgctca
      961 tcgctcatat ggtgcagcac gagctgctga tgatgctagc gccgccattt atcttgctgg
     1021 ctaaccccgt gccggtcttg ttgtggagct tgggtcggaa cccgcgattg caagccgggc
     1081 atcttctggc ccatcactca ccgatgcgcc gtgtccgcga tcgcttgggc tcgatgcccg
     1141 tcgcttggag cctctatgtc atcaacctgt gggcctggca ttatccggct ctttatgatg
     1201 cggctttgcg tgtgtcatgg atccatgatc tcgagcatgt tctttttttt ctcactgcac
     1261 tgattttctg gtggccggtg attcagccgg ggtctcgccc tgcacctatt caacacggac
     1321 ttcggatctt gtatctgttt ttcgccgcca ctcaagatac tatcctagcc ggcctgattg
     1381 cgctttcaag agaagttctc tacccgcact atgaaacggc tctgcggctg tgggatttga
     1441 ctcctcggga agatcaaatt ggcggcggga ttgtcatgtt cgccgtgggg agtacgacgt
     1501 atgcagtggc gatcttaatc ctcgtcaatg ccctgctggg tgagggcaga cgtaagcaat
     1561 caacgaaacg aaccttgagt gacggcgctg aaaaggttga aggccgcatc tgatgcgagc
     1621 accgccaacg caatccagat aagcgctaaa acgatttttt cagagagctc catcaggcgt
     1681 tacgttttcc ttgattgaac agaattgctg catttcgttt cttttattca tcctgagcgg
     1741 catcaacggg cgaataggac gaagaattat ttctttcgcg ccagcatcac cacccagcca
     1801 aagaagccta tgatggaaat gaaaacaatc gaaagggcga tcacgccgat cacctggagc
     1861 ccttccacgc cttcctgtgc gcccttgctg gataatccgg cccccaaccc tccccagccc
     1921 caaaattcaa acttagggcg caatccatcg aacggcccaa tgaggtagtg aatagaccag
     1981 aggaagagca cggcgtacag ccccagcagc cacctgttta cgatcccgtg gcgcgcgtgg
     2041 atttcgccgc cggcgtagtg ttcaagctca tccgagctgg gatgttcatg gttcatcttt
     2101 ttcaatctcc agaattcggt gcttgatatc ttcgagattg tcaaaggcgc cgtcgagaat
     2161 tccccagaac agaaagcagc aagccgcaag tcccatgaga aaggccacga taaattctat
     2221 caacgtgatt agttcgagca tggctctgcc ctcattgcga ggtcatttcg gccgtagccg
     2281 aaagacaaaa cattctggtc tcagtgataa tccacttctc tcattccgca atcctcagat
     2341 agttcttaga tttttctccc gccactgtcg tttcagcctg cgacggatcg aacgcggcgc
     2401 gccaggtcgg ggtttttaaa ttcggatcgt cgagcgcggc cttaacctgc tccgggatct
     2461 ggagcttttt gcgatgcaac ggatcggtat aggcggaaag gctcagcaca tagtaggaga
     2521 tggcccaacg ctgttcatcg gttaggttgg tggcgaaaga cggcatggct gcgccgttca
     2581 gccccgtgct catggtgcga aaaatatcgg taacgtcgga ccccgatttg aatattcccg
     2641 tcgtgaaatc gctcgcaagc aagggaaagc cccaatcgtc tttcaacgtt ggcgccgatg
     2701 ggccgcgacc ccaggggtag gtgaacggtt ggttgtcttt tatccccggc ccgccgtggc
     2761 agttccagca cctcccttcg ttgatgtaaa gtttttcgcc ttgagtgatt agttcttcgc
     2821 ttggcgcagg aggatcggga agggctaccg gcgcttcctt caagtttttg tctttccagg
     2881 ctggcgagaa agtcttaatg aataagatca ctgccaggcg ctgtttttcc ggaatctcgt
     2941 gccatggagg catcgccgtg ccgcggatgc cgcgggtaat cgtccggtag agatcgcctt
     3001 cggtcggcaa ggcgcctgac ggggtgacgc gaaatttgaa gaccgcctcc ctgaagtcgc
     3061 gtggccgggg atttagaaat gtcgctgccg gaccgttgcc gtctcctttc tcgccatgac
     3121 aaccgacgca gcgcctgagg taaacttctt tgccgtgagc aatccagtcc tcggactccg
     3181 gcatcgagaa ctggctgaca aaaacccgtt gaggctcaaa aagatcgcgc cattggccgc
     3241 gattcatgcc gagcttttga atataagcaa ccaatccctc cgcctctcgc gtcggggtga
     3301 cgagggtgat cgtgcttgtt ttgggtgggt cgggcaatgg atcgaagttt agcaccggaa
     3361 ctccatctcg ctcccgagcg aaagcgagcc cgtcgatgtt gggcaggatc tggatctcgg
     3421 ttccttgttt gaaggtaaaa atcttacgca gagaggaatc atctttaaag gcgagctttc
     3481 cgtctttttc gaccagttcg accgtgatct gctcgtagag ccaaggaaag gagggcatat
     3541 tcgagtccgg cacgactaag cgcgggttcc agtgatgggc aaaatgccaa tgatcgctgt
     3601 atttcagccc gacgcgtgtc aaatcgggtc cgattctgcg cgtgctaaac agatggggca
     3661 catcgaaggc gtattctccc gcctgagaga ctggccccca gcgttgatct tcgcctgtga
     3721 cgggacggat atattgagaa tggcaatacc aa
//
LOCUS       DASUTG010000318         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_50448, whole genome shotgun sequence.
ACCESSION   DASUTG010000318 DASUTG010000000
VERSION     DASUTG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50448"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1325)
                     /gene="amrS"
                     /locus_tag="VF089_07305"
     CDS             complement(249..1325)
                     /gene="amrS"
                     /locus_tag="VF089_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX6173807.1"
                     /translation="MIPTQYWHDLPDGRIQCDVCPRLCKLHNGQRGLCFVRANQNGAV
                     VLTTYGRSSGYCVDPIEKKPLNHYLPGTPILSFGTAGCNLSCSFCQNWDISKSREWDT
                     LSDAAAPETTARAARRLGCRSVAYTYNDPAIFMEYAIDVAKACREQGIKSVAVTAGYM
                     TPEPRSEFYKYMDAANVDLKAFSEDFYHRICGGHLQPVLDTLIYIKQETRVWLEITTL
                     LIPGHNDSEDEIDRMTRWGVASLGPDVPWHFTAFHPDWKMLDVRSTPASTLARARKIA
                     MANGVRYAYTGNVHDEEGASTYCHGCGATLIGRDWYEITAWNLDENGRCRQCGAACPG
                     VFEPRPGTWGAHRQPVRLNDFALP"
     gene            complement(1486..2649)
                     /locus_tag="VF089_07310"
     CDS             complement(1486..2649)
                     /locus_tag="VF089_07310"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6173808.1"
                     /translation="MDDRTRLPFGVLATAATLLSLVSCYATRIELWIPALREPIANWV
                     NTHVQAAIIVATAAAAVVGLMLDRKHHRSITPFAVGCAGFVLIAWSMYAPFGKIDDIV
                     GFVLLVSAVFLNQTKALHALTLNLKSQNTEIERQHKLLEQQSAQLAEWNRTLSQRVDD
                     QLSRIDRLGRLKRFFSPQLADLILEGNTQDPLKIHRREIAVVFLDLRGFTAFADRAEP
                     EEVVEVLHEYHTAMGELIAEFEGTLERFTGDGIMVFFGDPIAVENPLERAVRLSLAMR
                     DRIGTLTPRWNRRGYELGMGIGVSLGYATIGAIGFERRWDYAAIGRVTNLAARLCSEA
                     QPGQILINQPSLDGMETVLEIESVGDLTLKGFQRPILVSNILGLKSPRQARGA"
BASE COUNT          630 a          813 c          893 g          594 t
ORIGIN      
        1 tcagagacat cgaagatcga tccgaagctt tgcggtccag gattatggaa ctaatcgcgt
       61 tctagcgcac tgagcgtgcc aatgccggac tcgccgagga gagaggcggc aataggtgtt
      121 ggcgcgcggg ttcgatgcgg gaaagaaaac cttcatcact gggccgccgg aagagtccgc
      181 ttagcggagc ctgcgggcag tgaatcagcc ggctgcagtg accgagtgtc ttaggattat
      241 ttcgcatttt agggcagggc aaagtcgttc agacgcaccg gttggcggtg cgccccccag
      301 gtgccgggtc tcggctcaaa aacaccgggg caggcggcgc cgcactgccg acagcgaccg
      361 ttctcatcga gattccacgc ggtgatctcg taccaatcac ggccgatgag cgtggcgccg
      421 cagccgtgac agtaggtgct tgcgccttct tcgtcgtgaa cgttgccggt ataggcatac
      481 cgcacgccgt ttgccatggc gattttgcgt gcgcgtgcca gagtcgatgc gggtgtggat
      541 cggacgtcga gcatcttcca gtccgggtga aaggcggtga aatgccaggg cacgtcgggg
      601 ccgaggctgg caacgcccca ccgggtcatg cgatcgattt cgtcttcgga gtcgttgtgg
      661 ccgggaatca gcagtgtggt gatctcgagc cagacgcggg tctcctgctt gatatagatc
      721 aaggtgtcga gtaccggctg tagatgaccg ccgcagattc gatggtagaa atcctcgctg
      781 aacgccttga ggtcgacgtt tgccgcatcc atatacttgt agaactcgct acgcggctct
      841 ggagtcatgt aacctgcagt aaccgccacg gacttgatgc cttgctcgcg gcacgctttg
      901 gcgacgtcga tggcatactc catgaaaatg gcagggtcgt tataagtata agccacgctg
      961 cggcagccca gccgacgtgc ggctcgcgca gttgtttcgg gtgcggcggc atccgagagc
     1021 gtatcccatt cacgcgactt gctgatatcc cagttctggc agaaactgca ggagagattg
     1081 cagcctgccg tgccgaagga caggatcggc gtccctggaa gataatgatt cagaggcttc
     1141 ttctcgatcg ggtctacgca gtagccgctg gagcgcccgt aggttgtgag gacgaccgcg
     1201 ccgttttggt tggcgcgtac gaaacacaga ccgcgctggc cgttgtgcag cttgcatagc
     1261 cgcgggcaga cgtcgcactg gatacggcca tcgggcaaat catgccagta ctgggtcggt
     1321 atcaccgcct gcgggtcgag aatttcgggg gtcactggct aaaagataac gcctggccag
     1381 agcgcaggca atccaggagc ggttcgtctc ttgggcgtgc gcctcgttga attgaagtgg
     1441 ctatactggg ccgccggaaa acgtctacaa cgacgccgaa tgtcatcagg cgccgcgcgc
     1501 ctgtcgaggg gatttaagcc cgaggatatt cgaaaccaat atcggccgct gaaaaccctt
     1561 cagtgtcaga tcgcccactg attcgatttc caggacggtt tccatgccgt ccaaactcgg
     1621 ctgattgatc aggatttggc cgggttgcgc ttccgagcac aaacgtgcgg cgagattggt
     1681 tacgcgtccg atggccgcgt aatcccatcg ccgttcaaac ccaatagcgc cgatagtggc
     1741 atagcccagc gaaacaccga tgcccatgcc gagctcataa ccgcggcgat tccagcgcgg
     1801 cgtcaaagtg ccgatccggt cacgcatagc gagtgacaag cgtaccgcgc gctcgagcgg
     1861 attctccacc gcgatcggat cgccgaagaa gaccataatc ccgtcgccgg tgaagcgttc
     1921 cagagttcct tcgaactcgg cgatcaattc gcccatcgcc gtgtgatatt catgtaggac
     1981 ttcgacgacc tcctccggct cggcgcgatc ggcaaaggct gtgaatccgc gcagatcgag
     2041 aaataccacc gcgatttcgc ggcgatgaat tttcagcgga tcctgtgtat tcccctccag
     2101 gatcaaatcc gccagttgcg gcgaaaaaaa acgcttaagg cggccgaggc gatcgatgcg
     2161 cgagagttga tcgtcaacac gctgcgaaag cgtccgattc cactcggcga gctgcgcgga
     2221 ttgctgctcg agcagtttgt gttggcgttc gatctcggtg ttctgcgatt tgaggttcag
     2281 ggtcaatgcg tgcagcgcct tggtctgatt gagaaacacc gcggagacaa gcaggacgaa
     2341 tcccacgata tcgtcgatct tcccgaaagg tgcgtacatc gaccatgcaa tcaagacaaa
     2401 accggcgcag ccgaccgcaa aaggtgtaat gcttcgatga tgtttgcgat cgagcatgag
     2461 gccgaccacc gcggccgcgg ctgtggcgac gatgatggcc gcttgcacgt gagtattgac
     2521 ccagtttgcg atcggttcgc gcagtgccgg aatccataat tcaatgcggg tcgcgtaaca
     2581 cgagacaagc gataatagcg tagccgcggt tgccagcacg ccgaagggta aacgtgtgcg
     2641 atcatccatg atcacagttc tccataacgg tgcctgattc aactccgatg acagcagcgg
     2701 ccattttctc catgaatcta taatccacaa ttccgcggta gtgtcggaaa gccccatttc
     2761 agttccgaga tggagacaac ctggaggtta gccaatgacc cccctgtctg tcgacggcaa
     2821 gaggctccgg gtggcggcat cgacgctttc cataagagta gggaagttaa aaagatgcct
     2881 tagaggaaaa atcggtcaga cttgcgcggc tcgggcaatc caaagcgagc
//
LOCUS       DASUTG010000319         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1235363, whole genome shotgun sequence.
ACCESSION   DASUTG010000319 DASUTG010000000
VERSION     DASUTG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235363"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VF089_07315"
     CDS             complement(<1..1584)
                     /locus_tag="VF089_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6173809.1"
                     /translation="MAFIKAFLQWLFRRLYRVSIKGLNNYKEAGDRVLIVANHASYLD
                     GVLLAVFLPDRITFTVDSRIAKAWWVRPALALVDFFSIDTTNPLSTRSLIKFLSENRR
                     VMIFPEGRLTVTGGLMKIYQGPGLLADKSQALVLPIRIDGTQRTPFTHLGSSIRYRWF
                     PRITLSILPSRRISLPEQLRGRARRNRAATVLSDIMSEMMFATGNHHRPLFQALLDAR
                     RVHGGRHVIVEDNEHTPITYNQLISRAFILAKLFAQNTAPREYVGLLLPNTVATLIAF
                     FALQLRGRVPAMLNFTAGAQGIMTACEIAGIRTVYSSRRFIEAARLGDSVAAMEPHIG
                     VICLEDLRGKITILDKLMGWLDGYFAGRLYRRLPDNTGPEDPAVVLFTSGSEGLPKAV
                     ALSHHSLLANCSQIAARIDLNARDTVLTSLPMFHSFGLTAGTLLPLFCSVKIFLYPSP
                     LHYRRIPETAYENNVTLLFGTNTFLAGYGRFAHPYDFYSARYVFAGAEKLQEETRRTW
                     ADKFGVRLLEGYGATETGPV"
     gene            2059..2958
                     /locus_tag="VF089_07320"
     CDS             2059..2958
                     /locus_tag="VF089_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6173810.1"
                     /translation="MKQPKRVIVGHDLGVGGKAAMRSAAVVAERCGAALRIVHVVEPL
                     DAFQRLSHPLTSPYTLEDIVQKVGARLQALAESPEFDHLHSEYEVRTGKPFVELIAAA
                     RACLADLIVVGGTSGSEELLGGTSERIVRKTPIPVMVAKKPLSPEAKTFLIPTDFSSC
                     SRQAAEEAFVLGKCFDARLVFFHVLDSRRSLATRYAHELGVTVQMPLPSPEEMEPEWE
                     AFLSGLQLENVDWEKHTGEGQAANAIVGEAKHRQADIIVMGTHGRSGLSHTLLGSVTE
                     KVVRTAQCAVLTVRPDAFEFEMP"
     gene            complement(3106..3270)
                     /locus_tag="VF089_07325"
     CDS             complement(3106..3270)
                     /locus_tag="VF089_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173811.1"
                     /translation="MAASLPNLRSAPTVPWRQFKNAMAMPEKLFVALQAVVIGEMMRA
                     DVPLVGGTLQ"
BASE COUNT          804 a          868 c          970 g          754 t
ORIGIN      
        1 caccggaccg gtttccgtcg cgccataacc ttccaagagc cgcacgccga atttgtccgc
       61 ccaagtgcgg cgtgtttctt cctgcagttt ttcagcgccg gcaaacacat aacgggcgct
      121 gtagaaatcg taaggatggg cgaagcggcc gtagccggca agaaatgtgt tggtgccgaa
      181 cagaagcgtg acattgttct cgtatgcggt ctctggaatc ctacggtagt gcagcggcga
      241 agggtagagg aagattttaa ccgagcaaaa taacggcaac agggttccag cagtcaggcc
      301 gaaagagtga aacatcggca agcttgtcaa caccgtatcg cgcgcgttta gatcgatacg
      361 cgcggcgatc tggctacaat tagcaagcaa actatgatgt gacagcgcta ccgcctttgg
      421 cagcccctcg gaaccagaag tgaaaagtac cacggcagga tcttcgggcc cggtgttgtc
      481 gggtaaccgt cgataaaggc ggccggcaaa gtatccgtcg agccatccca tgagcttgtc
      541 caggatagtg atcttgccgc gcaaatcttc caggcagatg acaccgatgt gtggttccat
      601 tgccgcaacg ctgtcgccca gcctagccgc ctcgataaag cggcgtgagg aatagaccgt
      661 gcgtatgccc gcgatctcgc aggccgtcat aattccctga gcgcctgccg taaagttcaa
      721 catcgccggt acccgccccc ggagttgcag cgcaaagaag gcaatcaagg tagcgacggt
      781 gttgggcagt aataatccga catattctct tggcgcggta ttctgcgcga atagtttagc
      841 aagaataaaa gcgcggctga tgagctgatt ataggtgata ggggtgtgct cattatcttc
      901 gacgatgacg tgtcgtccgc catgaacgcg gcgagcatcg agaagagctt gaaacaaggg
      961 tcggtggtga ttgcccgtgg caaacatcat ttcgctcatg atgtcgctta gcactgtggc
     1021 agcccgattg cgccgggcgc gcccccgcaa ctgctcgggc agcgaaatcc ggcgcgacgg
     1081 cagtatcgat agcgtaatac gcggaaacca gcggtatcta atactactgc ccaggtgcgt
     1141 aaaaggcgta cgttgcgtgc catcgatgcg gatgggcagc accagagctt gcgatttgtc
     1201 agccagcaaa ccaggacctt gatagatttt catcaagccg cccgtaacgg tcaaacggcc
     1261 ctccggaaag atcatgaccc tacggttctc ggataagaat ttgatcagcg accgggtcga
     1321 tagcgggttg gtcgtatcta tcgagaagaa atccactaaa gcaagtgcgg gacgcaccca
     1381 ccaggcttta gctatgcgac tgtctactgt aaaggttatc cggtccggca gaaaaacggc
     1441 caggagtact ccgtccaggt aagaggcgtg gttggcgaca ataagaaccc gatcgcccgc
     1501 ttccttgtaa ttattcagac ctttgatact gacgcggtag agcctgcgaa aaagccactg
     1561 cagaaaagct ttgatgaatg ccatagctat aatttggcga gtttcggttt tttctcgggt
     1621 tcgttacaag tggtcgaatc caaaagcaac taatataccg tttgccagcc gtaaaccggc
     1681 agccgttgag aggccgcccc aaatcgcgcg acggcctcgc atcatcccgc tgtcccaatt
     1741 cagcggcgcc acacccacca gtgccgcgct cgttgagggc tgtattgttg accgcagagt
     1801 tgcgaattct gccggcattt tttgcatttg caagaaatcg ctaacaggat cgaatgaagt
     1861 ccggctgatt tgtaaaagat taggtggcac gtcagttgcg ctttaatatt cggcaaacgt
     1921 tcttgacctg atgccggcgg actctgactc gataattcaa aatatccgaa tagcgccgga
     1981 ggtctagcgc tttggatccg ccacgccagg acctcaactt ttcttcggat gaataaaata
     2041 gccagagaag ggagtcatat gaaacagccg aaacgggtta tcgttggtca tgatctcgga
     2101 gtcggtggaa aagcagcgat gcgatcggcg gcggtcgtag ccgagcggtg tggcgctgcg
     2161 cttcgaatcg ttcacgtagt cgagccgctc gatgcatttc aaaggttgtc gcatccgctg
     2221 acttctccat acacgttgga agatatcgtg cagaaggtag gtgctcgact ccaggcgttg
     2281 gcggaaagcc cagagtttga ccacctgcat tcggaatatg aggtgcgaac gggtaaacct
     2341 ttcgttgaat tgattgctgc cgcaagggct tgtctggcgg atctgatcgt ggttggtggc
     2401 acgtctggct ctgaagagct cctcggcggt accagcgaga ggatagttcg caaaacgccg
     2461 ataccggtaa tggtggcgaa aaagcccctc agtcccgaag ccaaaacatt tttgatccct
     2521 acggatttct cttcctgttc tagacaggcc gctgaggagg cgttcgtgct tggtaagtgc
     2581 tttgatgccc gcctggtttt ctttcatgtt ctggactcgc gtcgctcatt ggccactcga
     2641 tatgctcatg agctgggcgt gaccgtgcag atgccattgc cttcacccga agagatggag
     2701 cctgaatggg aagctttctt gtccggtttg cagctagaga atgtcgactg ggaaaaacat
     2761 actggagagg gtcaagccgc caatgccatt gtaggtgaag cgaagcacag gcaagcggat
     2821 atcatcgtca tgggcacaca tgggagaagc ggtttatcac acacgcttct aggcagcgtg
     2881 accgagaaag ttgtgagaac cgcgcaatgt gccgtactta cggttagacc agacgctttt
     2941 gagtttgaaa tgccatgagg agatcttttt cagtgtcgaa gttcgatcaa gcccagaaag
     3001 gagctctttg atcaactgag aaaggttgcc gctgcgcggc aagtccacta gcgctcggcg
     3061 gttacggcaa atctattcgg cacctttatc catcgaggag caacctcact gtagagtccc
     3121 accaacgagc ggaacgtctg ctcgcatcat ctcaccgatg acaacggcct gcagtgcgac
     3181 gaagagtttc tccggcatag ccattgcgtt tttgaattgt ctccagggca cggtcggtgc
     3241 gcttcgaaga ttcggcagag acgcagccat agttcgcgcc tcttccgtaa acggccgagg
     3301 cgagcagctt tactgggcaa gaaggatgag gagcttgcaa ggtgtattgc cgtgtcattg
     3361 catgtgattt cgacggaacc ggagccactg atggtc
//
LOCUS       DASUTG010000320         6878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_791145, whole genome shotgun sequence.
ACCESSION   DASUTG010000320 DASUTG010000000
VERSION     DASUTG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791145"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VF089_07330"
     CDS             <1..1041
                     /locus_tag="VF089_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX6173812.1"
                     /translation="SGIDFQNVRGLLVGFPSAVSGRWRYAERPQLLFDFRAPRLDITY
                     LLSQVDAESSEFYTNLQAQGRIAIASGKIKAIEFGELQSDLTIDHRVWRLSNLTTRAA
                     GGVVQAKASIDDRPDTLSIRTDSQIRGVPVQSFLRWFDMTTTEMTGTVNLAGSLETTG
                     NDDAERKKNLNGAFNLKIGEGIIHRMRILVQILNLLDLSRWFTLQLPDLTKDGIRFRS
                     IAADFKVKKGVYFTQNLVVDSDDLRMTGTGRIDVPKDDIDFLIAVRPFAGIDSAIHHI
                     PLLGRGIAAIKNSFLVASFNIKGPIEEPTITPAPLGTLSEWFWGVLGIPKNAIGLGDN
                     QNRQDDSKAAPR"
     gene            1038..1292
                     /locus_tag="VF089_07335"
     CDS             1038..1292
                     /locus_tag="VF089_07335"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6173813.1"
                     /translation="MRHRLVLYSRVDCCLCDEMKTVIRQVAERSPLEIEEIDIDSSAD
                     LKQKYGEEVPLLFINGRKSFKYQVTARQLAARLKSSWVRY"
     gene            1446..1808
                     /locus_tag="VF089_07340"
     CDS             1446..1808
                     /locus_tag="VF089_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173814.1"
                     /translation="MANQIVWCDIPVSELDRAVKFYSAVLDREVKKQDFPGMTVGVLP
                     HNDGEVGGCLFHSPEEKPSAQGVMIYLDATGRLDDAISAVTAHGGKILQAKHAIGPFG
                     FRCIILDSEGNRVALHSD"
     gene            complement(1822..3657)
                     /gene="typA"
                     /locus_tag="VF089_07345"
     CDS             complement(1822..3657)
                     /gene="typA"
                     /locus_tag="VF089_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX6173815.1"
                     /translation="MTQTQNRRDDIRNIAIIAHVDHGKTTLVDAMLHQSGIFRANEHV
                     AERVMDSMDLERERGITIMAKNTTIHYDKIKINIVDTPGHADFGGEVERTLAMVDGVM
                     LLVDASEGPLPQTRFVLKKALESKLPPIICINKIDRPDARVAEVLDEIVDLFIDLDAT
                     EDQLDFPVVYTNARAGTATRDLKRPGETLKPLFELIVQALPGPNVDPAAVTQFQANNL
                     DYSDYVGRLAVGRVKNGTLTPGQYTLCRVDGKQEIVKVTQVYGWQGLKRVEIPRTEGG
                     DIAAIAGIEDIGIGDTITDRENPHPLPPLRIDEPTIAMVFSANNSPWSGREGEFVTSR
                     KLRERLFFEQRRNVSLRVEETDLPDAFRVTGRGEFALAIVIETMRREGYELQVSKPTV
                     VTREIDGVTHEPMELLVIDVPEDFIGVVSQLLAVRKGKMTKMIHAGSSRVRLEFAVPS
                     RGLIGFRSRFLTDTRGTGIMNALFNGWAPWHGHIQSRTNGAMVADREGPTTPYAVFHL
                     QERGVLFIAPGTKVYEGMIVGEYSRDNDLNVNICREKKLTNIRAAGKDENILISPHRE
                     MGLEEGIEWIADDELVEVTPESIRLRKKILRQTDRPRRRQEKDDE"
     gene            3784..5523
                     /locus_tag="VF089_07350"
     CDS             3784..5523
                     /locus_tag="VF089_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6173816.1"
                     /translation="MGRLKTYLWRYWRRYLLGGLCLLGTATLVMWIPWWIREAVRILE
                     QGGALRDVTFYAAVIGLAAVAQGILRTCSRALIFNAGRNVEYDLRNDLFAHLQKLPSS
                     FYHARRTGDLMSRVINDISAVRVMLGPGVLNFANAPLYYVYALALMLSMDVRMTLAAL
                     APFPLLIYAAAKFRGRIMKSSLEVQQQMSALSSHVQENLSGMHVVKAYAQEKYQTQQF
                     VTLNEEFRGKSLEMARMRGIITPIMQGINGLTVVIVIWYGGVRVVRGDLLVADIVAFI
                     AYLNVLAWPTAAFGWMLSLVERGRAAMKRLEEILNTQPAIATVPGALPVAGLKNGIEF
                     REISFAYEQQANGHPILNHISFSLPVGKTIGLVGRIGSGKSTLAQLVPRLFDASSGEV
                     LIDGQDVRKLSLRDLRKALGYVPQEPFLFSTSLRRNLAFGRDNVSDQDLQRAVRIARL
                     DRDIENFPQGLDTVVGERGVTLSGGQKQRATLARALAIDPPVLILDDCLSSVDAQTEA
                     EILQGLRLILKEKTCLIISHRISAVKDADEILVFEDGRILDRGTHQQLVERGGLYAEL
                     HQQQRLTEELEQI"
     gene            5524..>6878
                     /locus_tag="VF089_07355"
     CDS             5524..>6878
                     /locus_tag="VF089_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX6173817.1"
                     /translation="MADPSHQEESLGKAYDLRLVRRLWRYIRPYKRLFFFAMLLLPIQ
                     QAFGLAQPYLMKIGIDQYIAQADLSGLQVVALLFLGAIIGETVMIFFHYYLTMAVAQR
                     CLADLRVELFSHVQKLPMAYFDRNPVGRLVTRMTTDVDVLQEMFSSGVMTLISDFVMV
                     VWIVGIMFYLHAELALVSLALIPPMALAINFFRIRARQTYRLIRERIARINAYLGEAI
                     SGMAIIQLFAREEKAFREFDEINAAHRDAYHLSNLYEAALYSMVEAAGSVSVALLLWY
                     GGGEVLHGVIGIGTVVAFKEYIHRFFVPLRDFSQKYAVMQSAMSSAERIFQLLDTPVT
                     IDSPKKPVIPKPFRGEVIFDDVWFHYKSDDPVLKGVSFRIQPGEKIAVVGATGSGKTT
                     TIKLLNRFYDVQKGSIQVSGVDVRDWDLQALRRHIGVVLQDVFLFSGDVRTNLALGDR
                     SV"
BASE COUNT         1470 a         1813 c         1891 g         1704 t
ORIGIN      
        1 agcggaattg atttccagaa tgtcaggggc ctgctcgttg ggtttcccag cgcggtcagt
       61 ggccgctggc gctacgcgga gaggccgcag ctgcttttcg acttcagggc gcctaggttg
      121 gatatcactt acctgttatc gcaggtcgat gccgaatcga gtgaattcta taccaatctg
      181 caggctcaag gtcgcatcgc tatagctagt ggaaaaatca aagctatcga gttcggcgag
      241 ttgcaaagtg acctcaccat tgaccaccgg gtgtggcgcc tgtcgaatct tacaacgcgc
      301 gcggcaggag gagtggtgca ggccaaggcc agcatcgatg atcgccccga tacgttgagc
      361 atacggacgg attctcaaat tcgcggcgta ccggtgcagt catttcttcg ctggttcgat
      421 atgaccacga ccgaaatgac tggaacggta aatttggctg gaagcctcga gaccaccggc
      481 aacgacgatg ccgaacgtaa aaagaatctc aacggcgctt tcaatctcaa gatcggcgag
      541 gggattattc accgcatgcg aattctcgtg cagatcttaa atctactcga tctgtcccgc
      601 tggtttacct tgcaactccc cgacttgacc aaggacggca ttcgctttcg ttccatcgcc
      661 gctgatttca aagtaaagaa aggcgtgtat tttacacaga atcttgtcgt cgatagcgat
      721 gatctgcgca tgaccggcac cggtaggatc gacgtgccca aagatgacat cgacttcctc
      781 atagccgtac gaccgtttgc aggcatcgat tcagcgattc atcacatacc acttcttggg
      841 cgtggtatcg ccgccataaa gaactcgttt ttagtggcaa gctttaatat caaaggtccg
      901 attgaggagc cgacgattac cccggcgccg ctcggcacgt tatccgaatg gttctggggg
      961 gttctgggaa tacccaagaa cgccattggt ttgggcgaca atcaaaatcg gcaggatgac
     1021 agtaaagcag cgccaagatg aggcaccggc tagtcttgta ctctcgagtt gactgttgcc
     1081 tatgcgacga gatgaaaacc gtgattcggc aagtagccga aagatctcct ttagagatcg
     1141 aggagatcga catcgacagc tccgctgatc tgaagcagaa gtacggggag gaggttccgc
     1201 tgctgttcat caacggccgc aaatcattca agtaccaggt taccgcgaga caattagcgg
     1261 cccgattgaa atcgtcttgg gttcgctact agagatggat ttccatgcta tcgttggcgg
     1321 accaggattg atctcatgcg agtgcaggat atacagaatc cgacaggacc aaacgcagta
     1381 aaccttgctg cggaacgatt ggctcctata ttgttacact catagctttt aaaagaggag
     1441 gagtcatggc caaccaaatc gtttggtgtg acatcccggt ttccgaactg gaccgagcgg
     1501 tgaagttcta ttcggcggta ctcgacaggg aagtcaaaaa acaagacttc ccgggaatga
     1561 ccgtgggtgt cctgccgcat aatgacggtg aggtgggtgg gtgcttattc catagtccgg
     1621 aagagaagcc atcggcgcag ggtgtgatga tctatctcga tgccactgga cgattggacg
     1681 atgcaatttc agcggtgact gctcacggcg gcaaaatcct tcaagcgaaa cacgccattg
     1741 gaccgttcgg attccgatgt atcattttag atagcgaggg aaaccgggtg gccttacact
     1801 cggactagct ttaaggcaac gtcactcgtc gtctttttcc tgtcgacgcc tgggtcgatc
     1861 ggtctgcctg aggatcttct tgcgcaaacg gatagattcc ggagtcacct cgacgagttc
     1921 atcatcggcg atccattcga ttccttcttc taagcccatt tcgcggtgcg gcgaaatcag
     1981 gatgttttca tctttcccgg cggctcggat gttcgtgagc tttttttcgc ggcagatatt
     2041 cacattcaaa tcgttgtccc gtgaatactc gccgacaatc attccttcat acacttttgt
     2101 gccgggcgcg ataaacagca cgccacgttc ctgcaggtga aaaacggcat acggggtagt
     2161 cgggccttcg cggtcagcga ccatggcgcc attggttcgc gattgaatgt ggccatgcca
     2221 cggcgcccac ccattgaaga gcgcgttcat gatgccggtg ccgcgagtgt cagttagaaa
     2281 acgggagcga aaaccgatga gaccgcgcga cggaaccgcg aattccaaac gcacacggct
     2341 cgatccggca tgaatcattt tggtcatctt gcctttgcgg acagcaagca gctgcgatac
     2401 cacgccgata aagtcctccg gcacgtcgat caccagcagc tccatcggct catgggtgac
     2461 tccatcgatt tctcgggtga caaccgtagg tttcgaaacc tggagctcat agccctcacg
     2521 acgcattgtt tctatgacga tggccagggc aaattcacct cggccagtga ctctgaatgc
     2581 gtcgggtagg tccgtttctt caacgcgcag gctcacgtta cggcgttgtt caaagaaaag
     2641 ccgttcgcgc agcttgcgcg aggtcacgaa ttcgccttcc cgccccgacc aaggcgaatt
     2701 gttggcactg aaaaccatgg cgatagtcgg ttcgtcgata cgcagaggcg gtagaggatg
     2761 ggggttttcg cggtcggtga tcgtgtcacc gataccgata tcctcaatgc cggcgatggc
     2821 agcgatatcg ccgccttctg tccttggaat ctcaacccgc ttcaatcctt gccagccgta
     2881 aacctgagtc accttgacga tttcttgctt accgtccacc cggcagagcg tatattgacc
     2941 gggcgtgagc gtgccgtttt tgaccctgcc tactgccagt cgtccgacat agtcgctata
     3001 atccaagttg ttggcttgaa actgtgtgac agctgcggga tcaacgttgg gtcccggcaa
     3061 tgcttgcaca atcagctcaa ataacggttt cagagtttcg ccgggccgtt tcaggtcgcg
     3121 agtggctgtg cccgcccgcg cattcgtata caccaccgga aaatcgagtt ggtcttcggt
     3181 tgcatccaag tcgatgaaca aatcgacaat ttcatcgaga acctcggcaa cacgcgcatc
     3241 cggccgatcg atcttattga tgcaaattat cgggggtaac ttggattcga gcgccttctt
     3301 caatacgaaa cgggtttgcg gcagcggccc ttctgaggca tcgaccaaca gcattacgcc
     3361 atccaccatt gccagagtgc gctcgacctc gccgccgaaa tcggcgtggc ctggagtatc
     3421 gacgatattg attttgattt tgtcgtaatg aatggtcgtg tttttggcca tgatcgtgat
     3481 gccgcgttcg cgctccaggt ccatggagtc catcactcgc tcggccacgt gttcgttggc
     3541 gcgaaagatg ccgctttggt gcaacatcgc gtccactaga gttgttttgc cgtgatcgac
     3601 gtgagcgatg atagctatat tgcgaatgtc atcgcgccga ttctgagttt gcgtcatacg
     3661 tctttattag tgagcaaccc agtattgtca agcgatactc tttgccggat ctcgtccgca
     3721 agtccgcgtg atttcgatat ttccttgata aggctgaccc cgtggagtat tttgatcatt
     3781 taaatgggtc ggcttaagac ttatctttgg cgctattggc gccgttacct cttgggcggg
     3841 ttatgcttat taggcacggc aactttggtg atgtggattc cttggtggat ccgtgaggcc
     3901 gtgcgcattc tcgaacaggg cggcgccctt cgcgacgtca cattttatgc cgctgttatc
     3961 ggtttggccg cggtggcgca ggggatctta cgaacttgct cccgggcatt gatctttaat
     4021 gccgggcgca atgtcgaata tgatctgcgc aacgatctat tcgcccatct gcagaaattg
     4081 ccttcatcgt tctatcacgc gcgtcgtacc ggcgatttaa tgtcgcgagt gatcaacgac
     4141 atctccgccg tgcgcgtgat gctcggcccg ggagtgctca attttgccaa cgctccgctc
     4201 tactatgttt atgctcttgc cttgatgctc tccatggatg tgcgcatgac tttggccgcg
     4261 ttggcgccgt ttccactgct gatctacgcg gcggccaaat ttcgcggccg gatcatgaaa
     4321 tcttcactcg aagttcagca gcagatgtcc gcgctcagca gccatgtgca ggagaatctt
     4381 agtggaatgc atgtggtcaa agcctatgca caggagaagt atcagacgca gcaatttgtc
     4441 acgctcaatg aagaatttcg cggtaagagt ctagagatgg cgcgaatgcg cggcatcatt
     4501 accccgatca tgcaaggcat caacgggctg acggttgtga tcgtgatctg gtatgggggc
     4561 gtgcgcgtgg tgcgcggcga tttactggtg gcggatattg ttgcctttat tgcttatcta
     4621 aacgttttgg catggccgac tgccgccttc ggatggatgc tgtctctagt ggagcggggc
     4681 cgtgcggcga tgaagcggct ggaggaaatt ctcaacaccc aaccagccat tgccactgtg
     4741 cccggcgcac tgccggttgc ggggctaaag aacggaatcg agttccggga aatatccttt
     4801 gcctacgagc agcaggctaa tggtcacccg attctgaacc acatcagctt ctcgttgccg
     4861 gtgggaaaaa ctatcggttt ggtcggccgg atcggttccg ggaagagcac cttagcccag
     4921 ctcgtgccgc ggctgttcga tgcatcatcg ggcgaggttc tcatagacgg tcaagatgtt
     4981 cgtaagctgt cgctgcgaga tttgcgcaaa gcgttgggtt atgttccgca agagccgttt
     5041 ctcttttcaa cttccctcag gcgcaatctt gccttcggcc gggacaatgt ttctgatcag
     5101 gatttgcaac gcgctgtgcg cattgcccgg ctcgaccgcg atatcgagaa tttcccgcag
     5161 gggctcgata ccgtggtcgg cgaacgcggg gtcactcttt ccggtggtca gaagcagcgg
     5221 gcgaccttgg cgcgcgcatt ggcgatcgac cctcctgtgc ttattctcga tgactgtttg
     5281 tcgagcgttg atgcccagac cgaagcggaa attttgcagg gactacggtt gatactgaaa
     5341 gagaaaacct gtctgatcat ttcccaccgg atttccgccg ttaaagatgc cgacgaaatc
     5401 cttgttttcg aggacggtcg aattcttgac cgcggcaccc accagcaact ggtggagcgt
     5461 ggcggacttt atgccgagct ccaccagcag caacgcttaa ccgaggagtt ggagcagatc
     5521 taagtggccg atccctcgca ccaagaagaa agcctcggca aagcctacga tctgcggctc
     5581 gttcgacggc tgtggagata catcaggccg tacaagcgct tgttcttttt tgccatgtta
     5641 ctgctgccga tccagcaagc cttcggcctg gcgcaacctt acctgatgaa aatcggcatc
     5701 gatcaatata tcgctcaggc cgatctatcg ggactgcaag tcgtcgccct gcttttcttg
     5761 ggagcaatca tcggcgagac cgttatgatt ttttttcatt actatctcac catggctgtg
     5821 gcccagcgct gcctggccga tctccgcgta gagctattct cacacgtgca gaagttgccg
     5881 atggcttatt tcgatcgcaa tccggtcggc cgattagtca cccgcatgac caccgatgtc
     5941 gacgttttac aggaaatgtt ttcttccggg gtgatgaccc tgatctccga tttcgtcatg
     6001 gttgtatgga tcgtgggcat catgttctat ctgcacgccg aactggcttt ggtttcattg
     6061 gcgctgatcc cgccaatggc tttggccatc aacttttttc gtatcagagc gcgccagaca
     6121 taccgtctga ttcgcgagcg gatcgcgcgc atcaacgcct atctcggcga ggcgatctcc
     6181 ggcatggcca tcattcaact gtttgcccgc gaggaaaaag cctttcgtga gttcgacgaa
     6241 atcaatgcgg ctcaccgtga cgcttatcat ctctccaacc tttatgaagc ggcgctttac
     6301 tcgatggtgg aagctgccgg ctcggtaagc gtggcattac tgctgtggta tggaggcggc
     6361 gaagttttgc acggcgtcat cggcatcggc accgtcgtgg cattcaaaga atacatccat
     6421 cgattcttcg ttccgttgcg tgacttcagc cagaaatatg cagtgatgca gtctgccatg
     6481 tcgtcggcag aacgaatttt tcagcttctg gatacgccgg ttacgattga tagcccgaaa
     6541 aaacccgtta ttcccaagcc gttccgcggc gaagtcatct ttgatgatgt ctggtttcac
     6601 tataagtccg acgacccggt tttaaagggg gtgtctttcc gcattcaacc cggagaaaag
     6661 atcgcagtcg tcggagcgac aggctcaggt aagacgacaa ccatcaagtt gctcaatcgt
     6721 ttttacgacg tacagaaagg atcgattcag gttagcggcg tggatgtccg tgattgggat
     6781 ctgcaggcgc tgcgaaggca tatcggagtt gtcttacagg atgtgtttct tttctccgga
     6841 gatgttcgca ccaacctggc gctcggtgac cgttcggt
//
LOCUS       DASUTG010000321         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_940910, whole genome shotgun sequence.
ACCESSION   DASUTG010000321 DASUTG010000000
VERSION     DASUTG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940910"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1011
                     /locus_tag="VF089_07360"
     CDS             277..1011
                     /locus_tag="VF089_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6173818.1"
                     /translation="MDNLMFWLISLIAGIIIYRLLTRRQVTPTARVTAMLRRYRALER
                     TGLSERDCLLQLLATRKGWERLSHRFLVAIVSRLGSKEDVMRFISLSEDHGYHRDRYP
                     ELATKVDLDTAIAEIACLFSRFGFRLQTEGRYKEAEFVQKLALRLQPDEYFTTLPLAA
                     TYHETGRHADALPLFEQGLARAKEFDQKPDPVKAEFTPAKCLAAEAELTKLRNRYNKM
                     YQACLKAAGDRTLTSSALLALIELFG"
     gene            complement(1102..2103)
                     /locus_tag="VF089_07365"
     CDS             complement(1102..2103)
                     /locus_tag="VF089_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173819.1"
                     /translation="MRTARLTVAASFLSFAFLLSHGSAQDRKPDRIRIGGGAVGAPQM
                     TMWFAKEANLYEKHGLLTEAIHIPGSSMALQAMLAGEVPIIQLGGAASMQANFAGADT
                     VIVATVLKRFLFSIFSRPDITSMADLKGKLFGATRFGTLSDFASRFALEKHGINPERD
                     ITMVQTGGQPETVTALITGKVQAAALSVPATMRAKKANMRELLDLATLEGTIHQNGVV
                     TTRKYLKTHEDVVRRFLKAYIEGAALAKKDKAFATRVMTRYLGTADREILDDAYERVV
                     RHLELPPYPAVEGVAVLLRSLEKAQPKARTAKPEDFIDDRLVRELDESGFIKSVMDK"
     gene            complement(2476..>3082)
                     /locus_tag="VF089_07370"
     CDS             complement(2476..>3082)
                     /locus_tag="VF089_07370"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6173820.1"
                     /translation="RPKPVAIVGLGAGAMACYGQHGQDFVFYEIDALVERIARDQALF
                     TYLRDCPPRVSVHIGDARVTVASAADQAYSVFVLDAFSGDAIPIHLLTREAVELYLSK
                     LMPEGVLLFHISNRHMDLAPVVDRIAAELKLTAFLRHDAEVSERERAQGRQPSTWAIL
                     ARQPKRLAPFRNDPKWQLLKGDRKGELWTDHYANIFQVLRW"
BASE COUNT          666 a          836 c          870 g          710 t
ORIGIN      
        1 ctagaagcaa gagtaagatc aaggctttca tgattccctc ctgatctgct tagactcatt
       61 ttctcggctt ctgttaacag accggtgacc aagctccgac tagaccacat agagaggatt
      121 caacggttac gcagagccgg gtttactacg atcttctctg aagatttatt cggatcgcca
      181 gaggtgcgct gagtagtcag tgatcgccac ttcgaccgtt tgttatgact tgacctggcg
      241 gggatttgcg tgtttttcat caccgaggag gtcagaatgg ataatctgat gttctggctg
      301 atttcgctta tcgcaggaat tatcatttat cggttattaa cccggcgcca ggtcactcct
      361 acggcgagag tcactgctat gctgaggcgc taccgggctc tggaaagaac cgggctttca
      421 gaacgagatt gtctcttgca gctgctggcg actcgcaaag gttgggaaag actatctcat
      481 cgttttctcg tggccattgt ctcgcggctc ggttccaagg aagacgtgat gcgttttatt
      541 tccctgtctg aagatcacgg ttaccataga gatcgttatc cggagcttgc gaccaaagtc
      601 gatttggata cggcgattgc agagattgct tgtttgtttt cgcgtttcgg tttccgactg
      661 caaacggaag ggcgttataa ggaggctgag tttgtgcaga agctcgcttt gcgacttcag
      721 cctgatgagt attttacgac tttgccgctc gcggctacgt atcacgagac cgggcggcat
      781 gccgacgctc tgccgctttt cgaacaggga ttggctcgcg ctaaagagtt cgatcaaaaa
      841 cccgatcctg tgaaggcgga atttactccg gcaaaatgtc tggctgccga ggcggagctt
      901 actaaactac gtaaccgtta caataaaatg taccaggcat gcctgaaagc tgccggtgac
      961 aggacgctaa cgagttctgc cctgctggcg ctcatcgagc tgttcggcta acagccctga
     1021 ctgaattgaa gcgcttcgcc ctcctgtgcc ccaggcaaac gtctttcaag atgtactcgc
     1081 gccggcggga atcggagctg ctcacttatc cattacgctc ttgatgaacc cgctttcgtc
     1141 gagttcgcgc accaggcgat cgtcgatgaa gtcctccggt ttcgcggtcc gcgctttcgg
     1201 ctgggctttt tccagagagc gcaacagcac tgcgacgcct tcaaccgccg ggtaaggggg
     1261 cagctcaagg tggcggacaa cccgctcgta ggcatcgtcg aggatctcgc gatccgcggt
     1321 gccgagatac cgagtcatga cgcgggtcgc aaaggcttta tcttttttgg ccagcgccgc
     1381 tccttcaatg taggccttga gaaaacgccg caccacgtcc tcgtgagtct tgagatactt
     1441 tctggttgtt accacgccgt tctggtgaat tgttccctct aacgtggcca agtcgagcag
     1501 ctcgcgcatg ttggccttct ttgcacgcat ggttgccggc accgacaggg cggccgcctg
     1561 gaccttaccc gtgatgagcg cggtaacggt ttcaggttga ccgccggtct gtaccatggt
     1621 gatgtcgcgc tcaggattga tgccatgttt ctccagagca aaacgcgagg cgaagtcgga
     1681 cagcgtacca aagcgcgtgg cgccaaacag cttgcccttc aaatctgcca tacttgtgat
     1741 atcgggacgg ctgaagatcg aaaacagaaa tctcttcaaa acggtcgcca cgatgaccgt
     1801 atcggctccg gcaaaattgg cctgcatcga cgccgcgccg ccaagctgga taatcggcac
     1861 ctcacctgcg agcatagcct gcaacgccat ggagctgccc ggtatgtgga tggcttctgt
     1921 taaaagccca tgcttctcgt agaggttggc ttccttggca aaccacatcg tcatctgcgg
     1981 cgcgcccacc gcgccgccgc cgatgcggat tcgatcgggt ttcctgtcct gggcagaacc
     2041 gtgcgagagc aaaaatgcaa agctgagaaa acttgccgct acggttaatc gtgccgtcct
     2101 catcgttacc tccgaagtaa gggagtttac cggcgcttga ttagcagaag tctgctaatc
     2161 ccgcaattgg tttatacggt caaacagagc atagatgctg gttaacaagt caggttacca
     2221 agagccttgc caaggcgctg cgcttcagac gcatcgaaga tgccgaagat ggctatcgca
     2281 cgatggtcaa tctatacgac cgtaagatta tcccagcgtc gatggaattc gtaatgtcat
     2341 tcgactgttg ggcgttaaca acgagaagat tcgccgcctc aaggcggaag agttgatcga
     2401 ggacggcgcg gtgcggcgga tggagaagga agggcggttc tagcagttca cattattcat
     2461 tgtcttggac accgcttacc agcgcagcac ctgaaagata ttagcgtagt ggtcggtcca
     2521 caactcgcct ttgcgatcgc ctttcagtaa ttgccacttg ggatcgttcc ggaacggcgc
     2581 aagccgttta ggctggcgag ccaggatcgc ccaggtcgat ggctgccggc cttgcgctcg
     2641 ttccctctcg ctcacttcgg cgtcatgccg caggaatgcc gtaagtttta gctcggcagc
     2701 gatgcgatcg acgaccggcg ccagatccat gtggcggttg gagatgtgaa acaacagcac
     2761 gccttccggc atcagcttgg aaagataaag ctcgacggct tcgcgagtca gcagatgaat
     2821 cggtatggca tcgccgctga aggcgtcgag aacgaagacg ctgtacgctt gatccgccgc
     2881 actggcgact gtcacccggg catcgccgat gtggaccgac acccgcggcg ggcagtcgcg
     2941 cagataagta aacagagcct ggtcacgtgc gatgcgctca accaaagcat cgatctcgta
     3001 aaaaacgaaa tcctgcccat gttggccgta gcaagccatg gcgccggcac ccagtccgac
     3061 gattgcgacg ggcttgggtc gg
//
LOCUS       DASUTG010000322         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_495294, whole genome shotgun sequence.
ACCESSION   DASUTG010000322 DASUTG010000000
VERSION     DASUTG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495294"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..2049
                     /locus_tag="VF089_07375"
     CDS             121..2049
                     /locus_tag="VF089_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX6173821.1"
                     /translation="MLKEITLRYRRIPIVAFHMALIVLANYLAFWIRFDGVIPANEKE
                     IMLRMIPWLMLIRGLTFVPFQLYKGLWRYTGIWDLRNVIVGVIASTILFYVLVHSLLG
                     LRAYPLSIFIIDSLLLIFLMGGSRLTRRLVYGIGQAKEGRRLLIYGAGDAGEMIVRDI
                     RNNSSFYGYQPIGFVDDDSKKIGRRIHGVQVFGRREDLPRIITEEKPDELVIAIPSAK
                     PVMVREVVKILEPFKIPIRTLPRVSELRNGNVGVKQMRDLAIEDLLDRAPVGLDLEPV
                     RTLVKGKRILVTGAGGSIGSELSRQIARYQPAQLVLLDNGESALYDINTELDRSVPSF
                     ERVAVLADIRDARAIEEIFASHAPQIVFHAAAYKHVPMMETHPNEAVLNNVVATHRLM
                     RFAIQYEAERFIQISTDKAVNPTNVMGATKRVAEMCIQALAKSCEQSRTVFTAVRFGN
                     VLGSNGSVVPLFLKQIEQGGPVTVTDAEISRYFMTIPEAVLLVLQAATLARGGDIFVL
                     EMGEQIKLMDMARHLIRLAGFVPDEDIPIHVVGLRPGEKLREELVGMDEMLIPAEVEK
                     IFRVQSAWLADLPFLTDKIDEMEQMALKGQSRRALALLYETVPTFRPIDPRTAKQIRQ
                     PGTNKESTRTWKLASRSA"
     gene            2266..2592
                     /locus_tag="VF089_07380"
     CDS             2266..2592
                     /locus_tag="VF089_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX6173822.1"
                     /translation="MPQAIASSKLDGLRLKRRMGVQSRLVEGEMVVLDREHDLIHQLN
                     KTATFIWEHCDGQQSAAEIANAVCENFEVDEATAIREVIQTLERLQSLRLIGDDQTDL
                     KPERRN"
     gene            2595..2771
                     /locus_tag="VF089_07385"
     CDS             2595..2771
                     /locus_tag="VF089_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173823.1"
                     /translation="MSEVKIKRREFVKRAAYAAPVILTLKAMPAFAASGSGSPRSSKG
                     DPSDRKATKAPAKS"
     gene            2884..3687
                     /locus_tag="VF089_07390"
     CDS             2884..3687
                     /locus_tag="VF089_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173824.1"
                     /translation="MAIDCRDRRAAELILANYAAMEGHPGDNPDLQYEVRRRGHGSQF
                     WLTRQGYELVTASDEGELLFILEKELTIELQKIRRELYFLHAAALSLADKALVIAAGS
                     GTGKSTTAWALLHHGFDYLSDELAPVDLDSLRLEPYPHALCLKREPPLPYLLPARTLR
                     MSSTLHVPVTQLPNSTVSQPTPLSAMFFLEYCPELKIPTAHRITKAEAAARLFVNALN
                     PLAHEQDGLAGAAVIAQKVPSFHLKSTELGLTCELVKAVLHQLDIKNTH"
     gene            3757..>4442
                     /locus_tag="VF089_07395"
     CDS             3757..>4442
                     /locus_tag="VF089_07395"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6173825.1"
                     /translation="MSSSRPQLISLLEAISSSREDLALDDFDDVTVAWAIQSGLGPLL
                     YRAVRRNAQSLASPTWNSLKAADLTARIVIGEHFQAVREIADACRSSLPPLTLLKGIS
                     ISEQCYPEAHLRLMRDIDLLVAKESLSTVTSALQGLGYRQLSNSALRYDKHHHLEPFF
                     HQDKQVWVEIHHALFSAERRASSASIFSRENLAAQLRPSRFQGAEVCRLSLELQLVYL
                     ASHWAQDFMP"
BASE COUNT         1021 a         1151 c         1236 g         1034 t
ORIGIN      
        1 cagagatcct gctggtgagt ttagagtcca agtcgtattt ttaccggttt ccatgctctg
       61 atcacgctat cgccagaggc cgcaccgcta ccggtcatag acaacgctga ggcaaattag
      121 atgttgaaag aaatcacact gaggtatcgt cggattccga tcgtcgcctt ccatatggct
      181 ttgatcgttt tggcgaacta tctggctttc tggattcgct tcgacggcgt gattcccgct
      241 aacgaaaagg aaatcatgct gcggatgatt ccgtggttga tgttgatccg cggcctgacg
      301 ttcgtgccgt ttcagcttta caaaggtctg tggcggtata ccggaatttg ggaccttcgg
      361 aatgtcattg tcggcgtaat cgccagtacg attttgtttt acgttctggt acattctctc
      421 ctcgggcttc gcgcttatcc tctttcgata tttatcattg actctcttct gctcattttt
      481 cttatgggcg gcagccgttt gacccgccgt ctggtttacg gcatcggtca ggcaaaggaa
      541 ggtcggcggt tgctgatcta cggcgccgga gacgccgggg aaatgatcgt ccgagatata
      601 aggaacaatt cttcttttta cggttaccag ccgattggct ttgtcgatga cgattcgaaa
      661 aaaatcggcc gcagaattca tggtgttcag gtcttcggca ggagagaaga tctgccccgg
      721 attatcacag aggagaagcc cgatgagctt gtgatcgcca tcccgtcggc caagccggtg
      781 atggtccgcg aagtagttaa gatactcgaa cctttcaaga ttccgatcag gacccttccg
      841 cgggttagcg aattgcgaaa cggcaatgtc ggcgttaagc agatgcgcga tctggcgatt
      901 gaggatttgc tcgatcgagc gccggtaggc ctggatctcg aaccggtgcg gactctggtg
      961 aaaggcaaac ggattctggt taccggcgcc ggcggctcga ttggttccga attgagccgg
     1021 cagattgcac gatatcaacc ggcgcagttg gttcttttag acaatggcga aagcgccctc
     1081 tatgatatca acaccgagct tgatcgctcg gtgccgagct tcgagcgtgt ggcggttctt
     1141 gccgacatca gagatgcacg agccatcgaa gaaatttttg catcccatgc tccacaaatt
     1201 gtctttcacg cggcagcgta caaacacgtg ccgatgatgg aaacccatcc caatgaagcg
     1261 gtcctcaaca acgtggttgc cacgcaccgg ctgatgcggt ttgccatcca gtacgaggcc
     1321 gaacggttca tccagatttc taccgataaa gcggtcaatc caaccaatgt gatgggagct
     1381 actaaacggg ttgccgagat gtgcatacag gcgttagcga agagctgcga acaaagccga
     1441 acggttttca ccgcagttcg ctttggcaat gttttgggca gcaacggcag tgtggtgccg
     1501 ttgtttttaa aacaaattga acaaggcggg cccgtgaccg tcaccgacgc tgagatcagc
     1561 cgctatttca tgaccatccc tgaagcagta ttgctggtgc tccaagccgc gacgcttgct
     1621 cggggcggtg acatcttcgt tctggaaatg ggcgagcaga tcaagttgat ggacatggcg
     1681 cgccatctca ttcgcctggc cggcttcgtt ccggacgagg acatcccgat tcatgtcgtc
     1741 ggtcttcgac ccggtgaaaa actgcgtgaa gagttggtgg gcatggacga gatgctcatc
     1801 cctgccgaag ttgaaaagat atttcgggtg caatctgcct ggttggcgga tctgccgttc
     1861 ctcaccgata aaattgacga gatggaacag atggcgctta aaggccagtc gcgcagagcg
     1921 ttagctttgc tctacgagac cgtaccgaca ttccggccga tcgatcctcg gaccgcaaag
     1981 cagatccgtc aaccaggaac caacaaagaa tccacccgga cttggaaact ggctagccgt
     2041 tcggcctaaa cgattcagaa gacaagggat caccggaatc ggaactagcg cagccgatca
     2101 gggaggtgat aacaatcagg ccatgagagt ccctgttaac ggagcttgca tttggttgcc
     2161 cgctgaggga tggaaagagc aagcaagctg gggcagcgtc tctcctactt tttgcacgcg
     2221 ccgggccagt gatctcattc agtgaagtgc ttcatcggag ttcctatgcc gcaggcaata
     2281 gccagttcaa agctcgatgg attgcgtctg aaacgtcgga tgggcgttca gagcaggctc
     2341 gtcgaaggcg aaatggtcgt tctggataga gagcacgact tgattcacca gctcaacaag
     2401 actgcaactt tcatttggga acattgcgat ggacagcagt cggcggccga aattgcgaat
     2461 gcggtttgtg aaaactttga agtcgatgaa gcgaccgcta tcagagaagt cattcaaacg
     2521 ctcgagagat tgcaaagtct acggctcatt ggagatgatc aaacagacct caaaccagaa
     2581 aggagaaact aactatgtcc gaggttaaaa taaagcgcag agagttcgtc aagagagcgg
     2641 cttatgcggc gccggtgatc ttaacgctga aggctatgcc tgcgtttgct gcgtcgggtt
     2701 ccggcagtcc gaggagctcc aaaggagatc cttccgacag aaaggcgacc aaagccccag
     2761 ccaagagcta gttcaatgac gagggcgcac cggagtggcg gtaaaccttg cttgcgctct
     2821 cgttaccctg ctgatcgtgt gcagtattgc cagaaactcg tgttccgcat ggtcgggtgc
     2881 tctgtggcaa tcgattgccg agatcggcgg gccgcggagc tgatactcgc aaactatgcc
     2941 gccatggaag gtcacccggg ggacaatcca gatctccagt acgaggtccg gcggcgaggc
     3001 cacggctcac agttctggct gactcggcag gggtatgagc tggtaaccgc aagcgatgaa
     3061 ggtgagcttc ttttcatatt ggaaaaggag ctcaccatcg aactacaaaa gatccgccgt
     3121 gagctgtatt ttcttcacgc tgcggcgttg agcttagcag acaaagccct tgtgatcgcg
     3181 gcgggctccg ggaccggaaa atcgaccaca gcctgggctc tgttacacca cggattcgat
     3241 tacttgagcg atgagctcgc tccggttgat ttggattctt tgcggctcga accgtatcca
     3301 catgcgctct gtttaaaaag agaaccgccg ctgccgtatc tcctgccagc gcgaaccttg
     3361 cgtatgtcat caactctgca cgttcctgta acgcaattgc cgaattccac tgtttcacaa
     3421 ccgacgccgc tgagcgcgat gttctttctt gagtactgcc cggaattaaa gatcccgacc
     3481 gcgcatagga tcaccaaggc tgaagcggcc gcgcggttgt ttgtgaatgc attgaatccc
     3541 ctggcccatg agcaagacgg cctcgccggc gcggcggtga tcgctcagaa ggttccatct
     3601 ttccatctta aatccaccga gcttggtcta acttgtgaac tagtgaaagc cgtcctacat
     3661 cagttagaca tcaagaacac acactgaacc gtcgataatg gtcgctgtga gcatgtgtgc
     3721 aagatagcgg cggcctatgg gtgatgcttc tagtccatgt ccagcagtcg tccgcaattg
     3781 atctcgctgc tcgaggcaat ctcgagcagc cgggaagatc tcgcgctcga tgactttgat
     3841 gacgtcacgg ttgcatgggc aatccaaagc ggcctcggac cgttattgta tcgcgccgtg
     3901 cgccgaaacg ctcagagctt ggcaagtccc acttggaatt cgcttaaagc ggcggatctg
     3961 acggcccgga ttgtcatagg agaacatttt caggcggtgc gagaaatcgc cgatgcctgc
     4021 cggtcgagcc tgccgccttt gacgttgctg aaaggcattt ctatcagtga gcagtgttac
     4081 cccgaggcgc atctaaggct catgcgcgac atagatctat tagtcgcaaa agaatcactg
     4141 tcgactgtca cgagcgcgtt gcagggactt ggttaccggc agctctcaaa tagcgccttg
     4201 cgctatgata agcatcatca ccttgagccg ttttttcacc aagacaagca agtctgggtc
     4261 gaaattcacc atgctttatt ctcggccgag cgcagagcca gcagcgccag tatcttcagc
     4321 cgtgaaaatc tcgccgctca gttacggcca tctcgatttc aaggagcgga ggtttgccgc
     4381 ttaagtttag agctgcagct cgtatatcta gcttctcact gggcgcaaga cttcatgcca
     4441 at
//
LOCUS       DASUTG010000323         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_941259, whole genome shotgun sequence.
ACCESSION   DASUTG010000323 DASUTG010000000
VERSION     DASUTG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941259"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VF089_07400"
     CDS             <1..857
                     /locus_tag="VF089_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6173826.1"
                     /translation="TQVSRARMIGALDEAIRAGQAQQRDQASNQIDIFTLLGTPGKGP
                     NRPGDVYPTVKEWSQQELLIFEKEALGFYITGHPLDKYDRMIKRLISGTAAGIKEKPM
                     SGEVKLAGVVSALKLRNTKKGDRYGSFNLEDKTGFLEVIAWPEIYKKSAELLSSDDPI
                     FIKGKLEVGEDRMQVIASEIMPLSEAAAKVRTLGNGSSNRNGHSDRVHLYVRESEISA
                     QELVQLREALLNCPGSSTVILHLLAQGVSETVIELPDQIRVASGPDLVVLVSKLFGPR
                     ISFRSLES"
     gene            857..2155
                     /gene="hflX"
                     /locus_tag="VF089_07405"
     CDS             857..2155
                     /gene="hflX"
                     /locus_tag="VF089_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6173827.1"
                     /translation="MRPHSNQQRKERAVLVGVELPSNDHKISLEYSLEELESLAQTAG
                     AEVAEKFTQQLRSVTPATLVGRGKVEQIRAKVDELHPDLVIFDEDLTPAQQRNLESAL
                     SVRVIDRSQLILDIFAQRARSNEGKLQVELAQLEYLLPRLTRQWTHLSRLGGGIGTRG
                     PGETQLEVDRRRIREKIGHLKRRLKTVERTRSLQRKERDNVPYATIALVGYTNAGKST
                     LMNALTRAGVLVEDKLFATLDPTIRSLRLPNGDKVMIADTVGFINKIPHSLIEAFKST
                     LEEVMSADLLLHLVDTSSPLVDEQIQVIEEVLGEIGAGDIPTIIVPNKIDLLPNGPQH
                     VFKNRGSQDVCSISAVTGEGLEALLKLIGTHLDRGKEKIHLSLSTAQGALLSLMRERG
                     RIENEVYEADQIHITAMVTPKLAGQLRKLARNNGSSDRDH"
     gene            2161..2727
                     /locus_tag="VF089_07410"
     CDS             2161..2727
                     /locus_tag="VF089_07410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6173828.1"
                     /translation="MLNLPNFLTLIRILSIPFFLVYLSYHRYLEALIIFIIGGVTDFL
                     DGLAARLMKQQTPLGAYLDPIADKLLVITSFIVLGLIGGIPMWLSIIVVSRDVLIITG
                     YGIIVFLTDERPEVKPSLIGKFSTLLQLFTLGVALALLHNPMLMHPWMRDIFIGFTAV
                     LTVVSGVQYIYRGFVWLQNKAPSISRIS"
     gene            2865..2939
                     /locus_tag="VF089_07415"
     tRNA            2865..2939
                     /locus_tag="VF089_07415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2897..2899,aa:Val,seq:gac)
     gene            2957..>3985
                     /locus_tag="VF089_07420"
     CDS             2957..>3985
                     /locus_tag="VF089_07420"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6173829.1"
                     /translation="MDSVRVTLSDGKSVEIGRGSRIYDLASLLSVDGGIIAAKINGSP
                     VDLSRILDEDCSLEWVSIDSPEGLDVLRHSTAHLMAQAVQSLFPGTQVTIGPTIQDGF
                     YYDFKRETPFTPEEIAKIEQRMEELARANLKVTREEMPKAQAIEMFRRMGEDYKVSII
                     EGITEDPVSLYRQGDWIDLCRGPHVPSTGKLKAFKITGVAGAYWRGDEKNEMLQRIYG
                     TSWPTKQALKEYLRLVEEAKKRDHRKLGQELGLFMISDAIGPGLPIWLPKGALLRSIL
                     ERYIVDIERSMGYQHVNTPQLARIDLYKRSGHWDHFKDNMYPPMAFENKEELVLRPMN
                     CPHHIIIYK"
BASE COUNT          985 a          973 c         1068 g          959 t
ORIGIN      
        1 cgacccaagt ttcacgagca cgcatgatcg gcgcgctgga cgaggctatt agggcgggcc
       61 aagcgcaaca gcgtgatcaa gcaagcaatc aaatcgacat tttcactttg ctgggtactc
      121 ccggtaaggg gccaaatcgg cccggcgatg tttatccaac ggtcaaagag tggtcccagc
      181 aggaattgtt gatcttcgag aaggaagcgc tgggtttcta cattaccggg caccctttag
      241 ataagtacga tcgaatgata aaaagactta ttagcggcac tgccgccggg atcaaggaaa
      301 agccaatgtc cggggaggtg aagctggccg gcgtagtctc ggcgctaaag ctgagaaata
      361 ccaagaaagg cgaccgctac ggcagcttca atttggaaga taaaaccggc tttctggaag
      421 tgatcgcgtg gccggagatc tacaaaaaga gcgcggagct cctgagctct gacgatccaa
      481 tattcataaa aggaaaactt gaggttggtg aagatcgcat gcaggtaatc gccagtgaaa
      541 tcatgccgct atccgaagcg gctgcaaaag ttcgcacgtt gggcaatggc agctccaatc
      601 gcaatggcca cagtgatcga gtgcatttat atgtgcgtga atcggaaatc tctgcgcaag
      661 aattggttca actgcgcgag gctctgctga attgccccgg ctcatcgacg gttattctcc
      721 atctcctggc gcaaggtgtt agcgaaactg tgattgaact gccagatcaa atccgggttg
      781 cctccggccc cgacttagtg gtccttgttt caaagctttt cggcccgcga atcagcttcc
      841 gttcactaga gtcttgatga ggccacactc taaccagcag cgcaaagagc gcgccgtttt
      901 ggttggcgtc gagctgccgt cgaacgatca taaaatttct ctggaataca gcctggagga
      961 gcttgaaagt ctagctcaaa ctgccggagc cgaagtagcg gaaaaattta cccaacagct
     1021 gagaagcgtt acgccggcta cattagtggg ccgaggcaag gttgagcaaa ttcgcgccaa
     1081 agtagatgaa cttcatccag acttggtgat ctttgatgaa gatttaacgc cggctcagca
     1141 gcgcaatctg gagtcagccc tcagcgtccg ggtcatcgac cgcagtcaat tgatacttga
     1201 tattttcgct caaagagcaa gaagcaatga aggtaaattg caggtggagc tggcacaact
     1261 ggaatatttg ctgccgcgtc tgacgcgcca gtggacacac ctttcacgac tcgggggtgg
     1321 tatcgggact cggggaccgg gtgagacgca gcttgaagtg gaccggcggc gcattcgcga
     1381 aaaaataggt catttgaagc ggcggctaaa aaccgtggag cgcacccgct ctttgcagcg
     1441 taaggagcgt gacaatgtgc cgtatgccac tatcgctctt gttggctaca cgaatgctgg
     1501 taaatcgacc ttaatgaacg ctctcacccg tgcgggggtc ttggtcgaag ataaactctt
     1561 cgcgactttg gatccgacga ttcgctcact gcgcctgccc aacggggaca aggtgatgat
     1621 cgctgacacc gtcggcttta tcaacaaaat tccgcattcg ctgatcgaag ctttcaagag
     1681 cacactggaa gaggtgatga gcgctgattt gctgctgcat ttagtcgaca cgagcagtcc
     1741 gctggtcgac gagcagattc aggttatcga ggaagtgctt ggtgaaattg gcgccggcga
     1801 tattccgaca attatcgttc ccaacaaaat tgacttgctc cctaacggac cacagcatgt
     1861 ctttaagaac cggggcagcc aagatgtatg ttcaatttcc gcggtgaccg gagagggact
     1921 ggaggcgctt ctcaaactca tcggtacgca tctagatcga ggcaaagaaa agattcactt
     1981 atcgctttca acggcgcaag gagctttgtt gtccctgatg cgagagcgag gtcgcatcga
     2041 gaatgaggtc tatgaggctg atcaaatcca tatcaccgcc atggtgactc cgaaattggc
     2101 tgggcagttg cgaaagctgg cgcgcaacaa tggttcgagc gatcgtgacc attaggcaag
     2161 atgctaaatc tgcctaactt tctaaccctg attcggattc tgagcattcc tttttttctt
     2221 gtctatcttt cctatcatcg ttacctcgaa gcgctgatca tttttatcat tggcggcgtc
     2281 accgatttcc tcgacggcct ggcggcaaga ttgatgaagc agcagacccc tttaggagct
     2341 tatctcgatc ccatcgcgga taagttgctg gtgataacct cgtttatcgt gcttggtctg
     2401 attggcggga ttcccatgtg gctttcgatt atagtggtaa gccgagacgt tttgatcatc
     2461 actggttatg gcatcattgt tttcctgacc gatgaacgtc ccgaagtgaa gccgagtctg
     2521 atcggtaaat tcagtacttt attgcagctc ttcactttag gtgtcgcttt ggctctgctg
     2581 cataatccca tgctgatgca tccctggatg cgcgatattt ttatcggctt tacggcggtt
     2641 ctgacggttg tctccggcgt gcagtacatc tatcgcggct ttgtctggct gcagaataaa
     2701 gccccatcca tcagtcgaat tagttgacat atcgcgatgg aattgataag gaaatcaggt
     2761 tcttgcgcgt catctaaagt ttcagtgaac agtatgtcac ggcggttgga caaacgtatt
     2821 tatgtcaatg attgcccttc gcagatcatc ttgccaagct ccacaggcac gtagctcagt
     2881 gggagagcgc ttccttgaca cggaagaggt cggcagttca atcctgcccg tgcctaccat
     2941 attcacttaa catcgaatgg acagtgttcg cgtaacatta agcgacggca aatccgttga
     3001 gattggccgt ggttctcgaa tttacgattt ggcgtcattg ctgagcgttg atggcggcat
     3061 catagcggcc aaaatcaatg gttctcctgt ggacctgagt cgaattcttg acgaagattg
     3121 ctcccttgaa tgggtttcga tcgatagccc cgagggcctc gatgtactgc ggcattcaac
     3181 cgcccatctg atggctcagg ccgtacagag cttattcccg gggactcagg tcaccatcgg
     3241 gcccacgatc caggatggat tttattacga tttcaaacga gaaacacctt ttacgccgga
     3301 agaaatcgcc aagatcgaac agcgcatgga agaattggcc cgagccaacc tgaaggtaac
     3361 tcgcgaggaa atgccaaagg ctcaggccat cgaaatgttt cggcgcatgg gcgaggacta
     3421 caaagtctcc atcatcgaag gcatcacgga ggatccggtt tcgctttatc gacagggtga
     3481 ttggattgat ctatgtcgtg gaccgcacgt gccttccact ggaaagctaa aggcatttaa
     3541 gatcactggc gttgccggag cctactggcg cggcgatgaa aagaacgaga tgttacagcg
     3601 gatctatgga acttcgtggc ctacgaagca ggctcttaaa gaatatttgc ggctggttga
     3661 ggaggcgaaa aaacgcgatc accgaaaact cggccaagag cttggcctat tcatgatttc
     3721 agatgccatc ggacccggac ttcccatttg gctgccgaaa ggagccctgc tgcgttcgat
     3781 tctcgagcgt tatatcgtcg atatcgagcg gagtatggga tatcaacacg tcaatacgcc
     3841 acaactcgcg cgcatcgatc tctataagag atcaggccat tgggatcact tcaaagacaa
     3901 tatgtatcct cccatggctt tcgagaataa agaagagctg gttttgcggc cgatgaattg
     3961 tccgcaccac atcattattt ataag
//
LOCUS       DASUTG010000324         5124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_51892, whole genome shotgun sequence.
ACCESSION   DASUTG010000324 DASUTG010000000
VERSION     DASUTG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51892"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VF089_07425"
     CDS             <1..444
                     /locus_tag="VF089_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX6173830.1"
                     /translation="LSRNFPQHKATFDRNLDAYLAELENAIERWQSVAAPLKGAKFVG
                     YHGDWAYFAKRFGLQQIGTIESKPGIEPTPNHLIALTQKMQQEKVQLIIYGPQSDRFP
                     RQLASQTGARLVRLQSTAGALPDTDSYIKFIDYNVKSLLAAMQKG"
     gene            499..1179
                     /locus_tag="VF089_07430"
     CDS             499..1179
                     /locus_tag="VF089_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6173831.1"
                     /translation="MGKLLPLVRLHRATLGYANEAVLHEIDLEMYPGDLVAMAGPNGS
                     GKTTLFRTILGFLPVLSGSLTRGCALHEFGYVPQSAALDATFPITVREIVEMGAYGRL
                     RPFERLPIAEKNRVDRVLEQVGAGDLAARAFFSLSGGQKQRILIARALMVGPKILILD
                     EPLSGVDTESRAAITELLLKINRQEGRAVFFSSHDLRMVRSVTKKILRVDRGRATWEE
                     GAAADHPW"
     gene            1173..2051
                     /locus_tag="VF089_07435"
     CDS             1173..2051
                     /locus_tag="VF089_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6173832.1"
                     /translation="MVNFFEFFSPSFLLFPALLGSAILAFVCPLVGAHLMLRRRIFLG
                     LTLPQIAACGVAFTFWLYHWVGWSHGVGGERLLAMAGSLVFTLVGMALLAYLDRHASG
                     SPEGRLAAAYALASALTILFVVFNPAGDLEILNLLKGEVISLSKEELKILAIAYSAIY
                     FGLVLFRREFLLNSFDPDLSFLLTDGGGRWSFWLYVVCGFSIAIGVIMAGPLLVFGFM
                     VLPPLAARTLARGMGSFFSLSSVFGVAIAISGFYLSVQLDLPLGPTDVTLACAVILLI
                     YAVQAGLRKCRSPVRL"
     gene            2183..3355
                     /locus_tag="VF089_07440"
     CDS             2183..3355
                     /locus_tag="VF089_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173833.1"
                     /translation="MASILIRNIGTLVTGKLEDPLRKVDSIYIEDGLVRAIGNGLKQK
                     ADQTIDANGITAIPGLIDSHSHPSIGEYTPAQNTLGWITNYMHGGVTALISAGELHLP
                     GLPLPPDARTALSVAIVTKRCYDSLRPSGVKVHAGTLLLVPGLTEKDFDEIQALGIKL
                     VKFIFYDYSLLPNGEAERYVNWAHARGIKVKIHSGGVSRSGVSQVAGFDIVKKIHPDI
                     IGHITGGPIPMPEADMLRIAGETECFMEICTSGNYRLALVLARYVKANNLHHRVLIGT
                     DTPGGTGVVPRGILREIAFISSVGEIEPEKSICMATGNVGRAHGLEIGILEEGRPADL
                     VLLHKITGSVAKDALESFAKGDLPGISTVIIDGEVRVAGRSQQTPPPERVASQSCC"
     gene            3421..4401
                     /locus_tag="VF089_07445"
     CDS             3421..4401
                     /locus_tag="VF089_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173834.1"
                     /translation="MSTFEFGVGLFPTEPLQKMIHLAKVSEESGYSHIWVGDSHLIWR
                     EAYVNMTAMMLNTTKVKFGTGVTNPLTRHPSVLASAYATLEEYAPGRTIVGIGLGDSS
                     VETMGLKPSTLANFEKSLEQMRALFEGKEAQLPSGKIHLLHPCKGKVPVYVAASGPKM
                     LELSGRIADGIIVLVGVADEYIVHARERIAAGAKAAGRKVEDINLVLWVPCAVSDSAP
                     AKDAVKAHVARVVAHPLPYVLDPTEQKVLEEIRKTYDYYHHMDQQANHAEVIPDWLVD
                     KFAIAGTVAECRTQIERIRKSGIQQISIIPYSPPGGSREKSIRGFAKAMS"
     gene            4436..4831
                     /gene="gloA"
                     /locus_tag="VF089_07450"
     CDS             4436..4831
                     /gene="gloA"
                     /locus_tag="VF089_07450"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX6173835.1"
                     /translation="MKIVHTMIRVNDLDESLKFYCDGLGMKLLRKREYESGRFTNVFV
                     GYGSEETEAVIELTYNWDTHSYDIGNGFGHIALGVPDVYKTCEELRAKGVKIVREPGP
                     MKHGSGSNAIAFLEDPNGYKIELTTRAEK"
     gene            complement(4933..>5124)
                     /locus_tag="VF089_07455"
     CDS             complement(4933..>5124)
                     /locus_tag="VF089_07455"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX6173836.1"
                     /translation="DKGIIMTTGTFTAEARKEATRDGVPPIELVDGQKMIDMFSELEL
                     GLKPIKTFEIDSDFFKEFQ"
BASE COUNT         1062 a         1412 c         1468 g         1182 t
ORIGIN      
        1 ctgtcgcgca acttcccgca gcataaggcg acattcgaca ggaacctcga tgcctatttg
       61 gccgagctgg agaacgcgat cgaacggtgg cagagcgttg cggcacctct aaaaggcgcc
      121 aaattcgtcg gctaccacgg cgattgggcg tactttgcca aacggttcgg actgcagcaa
      181 atcggcacca tcgagagcaa gccgggaatc gagccgacgc ccaatcatct catcgctcta
      241 acgcagaaga tgcagcagga aaaggtgcag ttgataatct acggcccgca gagcgaccgt
      301 ttcccgcgcc agctggcgag ccaaaccggc gctcgcctgg tgcgcttgca aagcaccgcc
      361 ggcgcactcc ctgacaccga cagctacatc aagtttatcg actacaacgt caagagtttg
      421 ctcgccgcca tgcagaaggg gtaaagaggc tgataatgaa ccgggatagc gatacgagtg
      481 agggagcgac tccatcttgt gggtaagttg ctccctctgg tgagattgca tagggcgaca
      541 ctcggctacg cgaacgaggc ggttttgcac gagattgatc ttgagatgta tcccggcgat
      601 ttggtcgcga tggcggggcc caacggatcc ggcaaaacga ctctattcag gacgattctc
      661 ggatttcttc ccgtgctgag cggctcattg acgcgcggct gcgcgttgca tgagttcggc
      721 tatgttcccc agagcgcggc gctggatgct acgtttccaa ttaccgttag agagatagtc
      781 gagatgggcg cctacggacg gcttaggccg ttcgagcggt tgccgatcgc cgaaaagaac
      841 cgcgtcgatc gcgtgctgga gcaagtcggc gcaggcgatc tcgcggcgcg cgcttttttt
      901 tctctatccg gcggtcaaaa gcagcgtatt ctgattgcgc gcgcgctgat ggtagggccg
      961 aagattctca tcctggatga gccgctttcc ggagtggata cagagtcccg ggcggcgatt
     1021 accgagctgc tgctcaaaat caatcggcaa gaaggcagag cggttttttt ctcgagccac
     1081 gatctcagga tggtccgcag cgttacgaaa aaaatcctgc gcgtcgaccg cggccgggcg
     1141 acttgggagg aaggggcggc ggcggaccat ccatggtgaa tttttttgaa ttctttagcc
     1201 cctcatttct gcttttcccg gcgttgctcg ggagcgcgat cctggctttc gtctgcccgt
     1261 tggtcggcgc ccacctgatg ctgcgccgga gaatttttct cggtctcacc ctgccgcaga
     1321 tcgccgcgtg tggcgtggcg ttcactttct ggctctatca ttgggtcggc tggagccacg
     1381 gtgtcggcgg cgagcggctt ttggcaatgg cgggctcgct ggtttttacc ctcgtcggca
     1441 tggccttgct cgcctatctg gatcgtcacg cgagcggatc gcccgagggc cggctcgccg
     1501 ccgcttatgc gctggcttcg gcgctgacga ttctgttcgt agtcttcaat cccgcgggcg
     1561 atctagaaat cctcaacctg ctcaaaggcg aagtgatttc gctctcgaaa gaggagctca
     1621 agattctagc gatcgcctac agcgcgattt acttcggttt ggttcttttc cgtcgcgagt
     1681 ttttgctcaa ctccttcgat cccgatctat catttttatt gaccgatggc ggcgggcgtt
     1741 ggagtttttg gctttatgtg gtgtgcggct tcagcatcgc catcggagtc attatggcag
     1801 gcccgctgct ggtgtttggc tttatggtgt tgccgccgct agctgcgcgg acgctcgcgc
     1861 gcggcatggg ttctttcttt tcgttgtcat cagtattcgg tgtcgccatc gcgatttcag
     1921 gtttttatct ttcggtccag ttagatctgc cgctcggccc caccgatgtc actctagctt
     1981 gcgcagtgat tttgttgatc tacgccgtgc aggctgggct gcgtaaatgc cgctccccgg
     2041 tcaggctgtg atggctctcg ttcgtcgcct gcgctgacga ctctcttctg tcttcatcga
     2101 aggattgtgc ggcaaagttc gcgcactggt aatcatctca ccctttacag tcctacgagc
     2161 tttgatatag ttccgcccaa gtatggcttc aattctcatc aggaacatcg gcacactggt
     2221 taccggtaag ttggaagatc ccttgcgcaa ggtggattcc atttatattg aagacggttt
     2281 ggttcgcgcc atcggcaacg gcttgaagca gaaagcggat cagaccatcg atgccaacgg
     2341 catcaccgcc atcccggggt tgattgattc gcattcgcac ccatcaatcg gtgaatatac
     2401 cccggcgcaa aacacgctcg gctggatcac caattatatg catggcggcg tgaccgcgct
     2461 gatctcggcg ggcgagttgc accttcctgg cctgccattg ccacccgacg cgcgcacggc
     2521 gttgtccgtc gccatcgtga ccaagcgctg ttatgacagt ctccggcctt cgggtgtcaa
     2581 agtccacgcc ggcactttgt tattggttcc cggactaacc gaaaaagatt tcgacgaaat
     2641 ccaggcgctg ggaatcaagc tggtcaagtt cattttttat gattacagcc tgctgcccaa
     2701 cggcgaggct gagcgctacg tcaactgggc gcatgcgcgc ggcatcaaag tcaaaattca
     2761 ttccggtggc gtatcgcgct ccggagtgag ccaggtcgcc ggcttcgata tcgttaagaa
     2821 aatccaccct gacatcatcg gccatatcac cggcgggccg atcccgatgc cggaagcaga
     2881 catgctgcgc atcgccggtg aaaccgaatg cttcatggag atttgcacgt cgggaaatta
     2941 tcggctggcg ctggttcttg cgcgttatgt caaagcgaac aacttgcatc atcgtgtgct
     3001 catcggcact gacaccccgg gcggcaccgg cgttgtgccg cgcggcatct tgcgcgagat
     3061 cgcctttatc tcttctgtcg gtgaaatcga gcctgagaaa tcgatttgca tggccaccgg
     3121 caacgtcggc cgggcacacg gtttggaaat tggcattctc gaagaggggc gaccggctga
     3181 tctggtcctg ctgcacaaaa taaccggctc cgtagccaaa gacgctctgg aatcctttgc
     3241 caagggcgat cttccgggta tctcgacagt gatcatcgac ggtgaagtcc gagtcgccgg
     3301 ccgcagccag caaacgccgc cacccgagcg ggtggcgagt caatcctgtt gttgaatttc
     3361 cgcgttagtt cgagaaaagc tgcttaatga acctgcacgg tggcttagga ggaatttttt
     3421 gtgagtactt ttgaatttgg cgtagggctt tttcccaccg agccgctaca gaaaatgatt
     3481 catctggcca aggtgagcga agaatcgggc tacagtcata tctgggtcgg cgattcccat
     3541 ctgatttggc gcgaggctta cgtgaacatg acggccatga tgctcaacac gacgaaggtc
     3601 aagttcggca cgggcgtgac taatccgctt acgcgccatc cgtcggtgct tgccagcgcc
     3661 tatgcgacct tggaagaata tgcgccgggt cgcaccatcg tcgggatcgg gctcggcgac
     3721 agctcggtgg aaaccatggg gttgaagccg tcgacgctcg ccaatttcga aaagtcgctg
     3781 gagcaaatgc gcgcgctgtt cgaaggaaaa gaagcgcagc taccgagcgg caagattcac
     3841 ctgcttcacc cttgcaaagg caaagtgcct gtttacgtcg ctgccagcgg gccgaagatg
     3901 ctcgaattgt ccggccgcat cgccgacggc attattgttt tggtcggcgt ggctgacgaa
     3961 tatatcgtcc atgctagaga gagaatcgcc gccggcgcca aagcggccgg caggaaagta
     4021 gaagacatca acctggttct ctgggtgcct tgcgctgttt cagacagcgc gccggctaag
     4081 gacgccgtca aggcccatgt cgctcgcgtt gtcgcgcatc cgcttcccta tgtgctcgat
     4141 cccaccgagc aaaaagtact cgaagaaatc cgcaagacct acgactatta tcatcacatg
     4201 gatcagcagg cgaaccatgc cgaggtgata ccggattggc tggtagacaa gttcgcgatt
     4261 gccggcaccg tcgccgagtg ccgcacgcag atcgaacgga tcagaaaatc gggcatacag
     4321 cagatttcga ttattccata cagcccgccg ggcggcagca gggaaaagag catccgcggg
     4381 tttgccaagg cgatgagcta aatcagtcgc cttatctaag tacgagggag acgcgatgaa
     4441 aattgttcat acgatgatcc gagtcaatga tctcgacgag agtttaaagt tttattgcga
     4501 tgggcttggc atgaagcttt taagaaagcg cgaatacgag tccggtcgct tcaccaacgt
     4561 tttcgttggc tatggttccg aagagacgga agcagtcatc gaactcacat acaattggga
     4621 tactcattcg tatgatatcg gcaacggttt cggtcacatc gccctcggcg tgccggatgt
     4681 ttataagacc tgtgaagagc tgcgcgccaa gggagtcaag atcgtgcgtg agccgggacc
     4741 catgaagcat gggagcggca gcaacgccat cgccttcctc gaagatccga acggctacaa
     4801 aatcgagctg acaacgcgcg ctgaaaagtg atttcccttc tcggtttact ccttgcgccg
     4861 gagcggccaa aaagtgtctt tatatgttct tgaacacggt cgcgtgtaac ggtgagtaag
     4921 ggtcccatcc catcactgga actctttaaa aaaatcactg tcgatctcga aagttttgat
     4981 aggcttcagg cctagctcaa gctcactaaa catgtcgatc atcttctgtc cgtcaaccag
     5041 ctcgatcgga ggaacaccat ctctcgttgc ctctttgcgt gcctcggcag tgaaagtccc
     5101 cgtagtcatg atgatccctt tatc
//
LOCUS       DASUTG010000325         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1533802, whole genome shotgun sequence.
ACCESSION   DASUTG010000325 DASUTG010000000
VERSION     DASUTG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533802"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF089_07460"
     CDS             <1..531
                     /locus_tag="VF089_07460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6173837.1"
                     /translation="RNRFGQHHPLALSASLCFEQSYSGIEGDSASSTELYALLSRLTN
                     ISLRQDLAVTGSVNQWGEVQAIGGVNEKIEGFYDICRVIGLTGTQGVLIPEANVRHLI
                     LRQDVIDAVAQGQFHIYPVRTIDEGLELLTGIPAAGGPGVEPINDAAARRLKELVVGL
                     KDFVALAHNGAAEMKE"
     gene            637..993
                     /locus_tag="VF089_07465"
     CDS             637..993
                     /locus_tag="VF089_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX6173838.1"
                     /translation="MIAYKTDKCYEKLQKRQGEITRTLEHVQKERRTVEENKKWIDKA
                     AYQSRCHLLDRLADWYLKETTIIQQALMRISEGRYGICVACREPIEPQRLETAPEAEF
                     CAQCQKLRESVIQRVA"
     gene            1260..4001
                     /locus_tag="VF089_07470"
     CDS             1260..4001
                     /locus_tag="VF089_07470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6173839.1"
                     /translation="MASVHPRFLPVPYQDSAEAGRLILRDGTSAEIRLAHPEDCRAMM
                     KFFAGLSGESRRRRFFGYGPPSSKLVNAFCDPSDLRKQLSLIVTRTGAAGQQIIATGN
                     YIALDDNTAEVAMAVDDSFHGKGIGTLLLERLTLLAVRNGFRRFRALTLSENKPMLDV
                     FLESGFECHRKHSDGHVEIDLSVIPNEISVARAELRDRASTIASLQAFFRPSSVALVG
                     ASRNPASIGGRILNALLGAGFSGPVYPVNPHMVSISGLTVYPTVAAIPQTVDLAVVAV
                     PRDAVLGVIDDCANRQVRALVIITAGFAEGGNDGVALQHQLIAKVRGYGMRMVGPNCL
                     GIMNADPAVSLNATFSPLFPAPGRIAFSSQSGALGLAVLALTRERQIGISNFVSVGNK
                     ADVSGNDLLQYWEEDKQTQVILLYLESFGNPRRFARIAKRVGRRKPIVTVKAGRTSAG
                     HRAAGSHTAALAASDVAVDSLFHQTGVIRAETLEELFDLATALSTQPLPGGRRVGILT
                     NAGGLGILCADSCEANGLIVPELHAETRTQLKAFLPASASVTNPVDMIASASAEHFSK
                     ALEILLPSAELDAVIVLYIDVGLADIGSMAKSIASRVADVRKATEAQKPVLACVISAE
                     ETRMPIKVDGENIPNFAFPESPARVLSKLVNYSEWKNQPPGIIPDFDDIEPAVARSLC
                     RRILHERGAGWLSAEEIRQVLAAMALPLARYVLCRTAEEAAYAGAEVGFPVALKLASR
                     EIIHKTEFGGVQLNLQNEDAVRRAFEYVSDQLAKRQMSKAMDGVVVQPMISGGVELMV
                     GVTQDRSFGPLIAFGLGGIHVEILKDVSFRVTPITDLDAAEMVRSIRGYRLLQGYRGH
                     PPADIPAIENLLLRVARLVEDVPEIGELDLNPVIALPPGGGCQVVDARIRINPESTR"
     gene            complement(4078..>4963)
                     /locus_tag="VF089_07475"
     CDS             complement(4078..>4963)
                     /locus_tag="VF089_07475"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEX6173840.1"
                     /translation="IRIGFDMAGGKKFLVDSGDLGRYGRPILKDPDSIEHADWLLLES
                     TYGNRTHPTNSEDELANVINKVAIAKGCLIIPSFAVGRTQEIIYSIRTLEDQGKIPAI
                     PVHIDSPMAINATDIYCAHSEEHDLDMKLLNEAKLCPLCSKKFYIHRTAEDSKAINDM
                     AGPLIIISASGMVTGGRVLHHLKRRLPDAKTTVLLVGFQAEGTRGRTLQNGMKEVKML
                     GEIIPIRAEVKTIDGFSAHADQGEILRWLGSFKKPPKKTFVIHGEPPASKALDLLLRE
                     ELKWNTEIPSIGQTVVLY"
BASE COUNT         1071 a         1315 c         1432 g         1145 t
ORIGIN      
        1 cgcaaccgtt tcggccagca tcacccgctg gcgctatccg ccagcctctg cttcgagcag
       61 tcgtattccg ggatcgaagg cgacagcgcc tcatcgaccg agctctatgc gctgctctcc
      121 cggttgacaa acatctctct acgccaggac ctcgcggtga cgggctcggt gaaccaatgg
      181 ggagaggtcc aggcgatcgg cggcgtcaac gaaaaaatcg aaggcttcta cgatatttgt
      241 agagtgattg ggctgacggg aacacagggg gtcctcattc ccgaagcgaa cgtgcgccac
      301 ttaattctgc gccaggacgt catcgatgcc gtggcacaag gtcaatttca catctacccg
      361 gtgcgcacaa tcgacgaggg gctggagttg ctaacgggta ttccggcggc gggtgggccg
      421 ggagtggaac cgatcaatga cgcggcggcg cggcggctga aagagttggt ggtggggctt
      481 aaagactttg tcgcactggc tcataacggc gccgctgaaa tgaaagaata agaaacctgc
      541 ccacgacgag caagtcttca cgtcgtagta gatgatcaag cggtcggtga gtagttatac
      601 gatgtgttcg ggaagcggaa tccgaaggag attaggatga tagcgtataa gaccgacaag
      661 tgttacgaga agctgcaaaa acgtcagggc gaaatcacga gaacacttga gcatgtgcag
      721 aaagaacggc gcacggtgga ggaaaacaaa aaatggatcg ataaagcagc ataccaaagc
      781 agatgccatc tgctcgatag attggcggac tggtacctca aggaaacgac tatcatacaa
      841 caagctctca tgcgtatcag cgaagggcga tacggtatat gcgttgcttg ccgcgagccc
      901 attgagccgc agcgtctcga aacagccccc gaagccgagt tctgcgcgca atgtcagaaa
      961 cttagggaaa gtgttatcca gcgcgttgct taggccgccg gggttagctc gcgctcttca
     1021 gcaggataaa cggtgccacc aatcgtttgt tcggttcctt acaccgtcga tcgggcgttc
     1081 tgctccggaa tatctcgtct attcgaagca caggagaaga tatttcagtt ccgttccggt
     1141 accactctgt gcgacaccgg cggtcctcga attgtttgtc tgtcagatag ttgccagcac
     1201 gtgaacttga tttcgggttc ctccttgaaa ctcctgtggt ggttgtttta caagagtctg
     1261 tggcttccgt tcatccacga ttcttgccgg tgccgtatca ggactctgct gaagcgggtc
     1321 ggttgatcct gcgcgacggc acctccgcgg agatccggct ggctcacccc gaagattgcc
     1381 gggccatgat gaagttcttc gccggacttt ccggggagtc cagaaggcgg cggttctttg
     1441 gctacggccc tcctagtagc aagttggtca acgcattttg cgatccttca gacttacgca
     1501 agcagctgag cctgatcgtc actcgaaccg gcgccgccgg gcaacagata attgccacag
     1561 ggaattacat cgcccttgat gacaacacgg cggaggtggc gatggccgtg gacgattcat
     1621 tccacggtaa aggaatcggt acattgctcc tggagcgcct gacattgctc gccgtgagaa
     1681 acggctttcg ccgtttccga gccctcactt tgagcgaaaa caagccgatg ctggatgtct
     1741 ttctggagtc cggcttcgaa tgccaccgca agcactcaga tggacatgtc gaaatcgatc
     1801 tttcggttat tcccaacgaa attagcgtcg cgcgcgccga actgcgcgac cgagcttcga
     1861 cgattgcctc actgcaagct ttctttcgac caagctcggt agctttggtc ggcgcctcgc
     1921 gcaatccggc aagcatcggc ggccgaattt taaatgcact acttggggct ggattttcgg
     1981 gcccggtcta ccccgtaaac ccacacatgg tctcgatcag cgggctcact gtttatccga
     2041 ccgttgccgc cataccgcag acggttgatc tggcggttgt tgcggtgcca cgcgacgcag
     2101 ttctcggcgt cattgatgat tgcgccaacc gtcaagtcag ggctttggtc attattactg
     2161 ccggatttgc ggagggtggg aatgatgggg tggctttgca gcaccagctc attgcgaaag
     2221 tccgtggcta tggcatgcgc atggtgggac ccaactgtct gggaattatg aacgcagacc
     2281 cggcggtgag tttgaatgcc actttttctc cactgttccc ggcgccaggc cgcatcgcgt
     2341 tttcatcgca gagcggcgcg ctgggccttg ctgtactcgc tttgacccgt gaacggcaga
     2401 ttggaatctc gaattttgta agtgtcggca acaaagccga tgtttcggga aatgatctgc
     2461 tgcaatattg ggaagaagat aagcaaacgc aagtcattct gctctacctg gaatctttcg
     2521 gaaacccgag gcgcttcgcc cgcatcgcca agcgagtcgg gcgccgcaag cctattgtca
     2581 ctgttaaggc cggaagaacc agcgccggac accgcgcggc tgggtcgcat accgctgcct
     2641 tggcagcaag cgacgtagcg gtggattcgc tttttcacca gaccggcgtc attcgcgcgg
     2701 agacgttgga ggagttgttc gatttggcga ctgcgctcag cactcaaccc ttaccgggag
     2761 gccgcagggt cggaattctc accaacgcag gcggtctagg aattctttgt gcggatagct
     2821 gcgaagccaa cggcctgatt gttcccgagc tccatgccga gactaggact cagttgaaag
     2881 cattcttgcc tgcgtctgcg agtgtcacca atcccgtcga catgatcgca tctgcgagtg
     2941 ccgagcactt cagcaaggcg ttggagattc ttctgccgag cgcggaactc gatgcggtca
     3001 tcgtgctcta catcgatgtc ggtctcgcgg atataggttc catggcgaag agcattgcct
     3061 ccagagtagc cgatgtaagg aaagcgactg aagcgcaaaa gccggttctg gcatgtgtta
     3121 tatcagctga agagacccgg atgccgatca aagttgatgg cgagaacatt cctaactttg
     3181 ccttcccgga aagtcccgcg cgcgttttga gcaaactcgt taactacagc gaatggaaaa
     3241 accaaccgcc gggaatcata ccggactttg atgacatcga acctgcggtt gcacggtcgc
     3301 tgtgccggag aatccttcac gagcgcggcg ccggctggct ctcggcagaa gagatccgcc
     3361 aggtgctcgc cgcaatggcg ttgccgttgg cgcgctatgt tctctgtcgc acagcggagg
     3421 aggcggctta tgccggggcc gaggttggat ttcccgttgc gcttaaactg gcgtcgcgcg
     3481 aaatcattca taaaactgag ttcggcggcg ttcaattaaa tctccaaaat gaggacgcag
     3541 tgcgccgcgc cttcgaatac gttagtgatc aactggcaaa gcgacaaatg tcgaaggcca
     3601 tggatggggt ggtcgtgcag ccgatgattt caggcggcgt tgagttgatg gttggagtca
     3661 cacaagatcg gtcttttggt cctctgattg cgtttggctt gggcggtatt cacgtagaaa
     3721 ttctcaagga cgtttcattc cgcgtgacgc caatcactga tctggacgcg gcggagatgg
     3781 tgcgaagtat tcgcggttat cgcctgctgc agggctaccg cggccatccg ccggcggaca
     3841 tacctgcgat tgagaatctg ctgttgcgcg tggcgcgatt ggttgaagac gttccggaaa
     3901 tcggcgagct ggatttaaac ccggtcatcg cattaccacc aggcggggga tgtcaagtgg
     3961 ttgatgcgcg cattcggatc aacccagaaa gcaccagata ggtatacgca ttgttgaatg
     4021 ccatcacgct agccgaacta tcccgcgtca atccacctgc gagtgcggga tccacgatta
     4081 gtacaagacg acggtctggc cgatgctggg gatctccgta ttccacttca actcctcacg
     4141 caggaggaga tccagagcct tactcgccgg cggttcgcca tggatgacaa aagttttttt
     4201 cggcggcttt ttgaaagatc ctagccagcg caaaatttcg ccttggtcgg catgggcgga
     4261 gaaaccgtcg atggttttca cttcggctct gatggggatg atttcaccca gcatttttac
     4321 ttccttcatc ccgttctgaa gcgttctacc gcgggttcct tccgcctgaa aaccaaccag
     4381 cagcacggtc gttttggcat cgggcagccg gcgctttaaa tgatggagaa cacggccgcc
     4441 tgtgaccatt ccgcttgcgg aaatgattat taaaggtccg gccatgtcgt tgatggcttt
     4501 cgaatcttcg gcagttctgt gtatgtaaaa cttcttggaa cagagggggc agagcttcgc
     4561 ctcgttgagg agcttcatat cgagatcgtg ctcttcagaa tgagcgcagt aaatatccgt
     4621 tgcgtttatc gccatggggc tgtcgatgtg cacgggaatc gccggtattt tcccttggtc
     4681 ttctaatgta cggatgctgt aaataatctc ctgggtccgt cccacggcaa atgatgggat
     4741 aatcaagcat cccttggcga tagctacctt gttgatgacg ttcgccagtt cgtcttccga
     4801 gtttgtcgga tgcgttcggt ttccgtaggt cgactccaac agcagccaat cggcatgttc
     4861 gatcgagtcg ggatctttta agattggcct tccataccgg cctagatctc ctgaatcgac
     4921 tagaaatttc tttccacccg ccatgtcaaa accgatgcgg ata
//
LOCUS       DASUTG010000326         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_495912, whole genome shotgun sequence.
ACCESSION   DASUTG010000326 DASUTG010000000
VERSION     DASUTG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495912"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..910
                     /locus_tag="VF089_07480"
     CDS             245..910
                     /locus_tag="VF089_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6173841.1"
                     /translation="MESLGDYQNCTILYVDDEEKSLVNFNRVFRNKFKILSAANAAEG
                     YRLLEEYRDDIALLMSDQRMPGEKGVEFLKRAREVHPNAVRILTTAYSDFDVAIEAMN
                     SGAVSKLVTKPWDIPQLEMILKEACDFFTAQREGEFLPGTDFSAQLKPTTHERGSEIG
                     ELRTLEQVARYLNVDKFTVYRLITQKKIPAFKVGNQWRFKQEMIDAWLMAKANIKKPK
                     SDY"
     gene            1356..>3403
                     /locus_tag="VF089_07485"
     CDS             1356..>3403
                     /locus_tag="VF089_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6173842.1"
                     /translation="MSQQFAEKIGDLLTQEGLLTAEQLAKSIAIQKQQDSTEPIGKIC
                     VSLGFVAPTDLEKVLIKHRHRIPLGELLVHLGLLTPGQVHSALEQQKKLNPHKKLGAV
                     LIEKGYIDEAALIRALYEQSQAGNCEKLKQGKFDSLVAMRRLSQRDLDLALREAQIQQ
                     RPVEILLMERYKLKKQEVGAALSVFYGCPFKEYDEKQPPAWDSIRGLNLNYLKAASWI
                     PLKVSERSVEVLIDDPTAYEKVQDIKRLFLGKQIKFAVGMRDDILRYFSTAKANLEKP
                     QSHESITTILGQLDVAAHDEADDDSLNDPAINENHSAIVRLVNQIITDACKKRVSDIH
                     IEPYGSQNDTEVRFRVDGHCSNYLRVPAVYRRALVSRLKIMAGLDIAERRKPQDGKIK
                     FRLPNREIELRVATIPTAGIGNEDIVLRVLAAGEPIPINELRMTSRNLNNFRELLAKP
                     YGLILCVGPTGSGKTTTLHSALNVINTAERKIWTAEDPVEITQRGLRQVQVNSKIGFN
                     FAAAMRSFLRADPDVIMVGEIRDRETAEIGIEASLTGHLVLSTLHTNSAVETITRLLE
                     MGMDPFNFADALLGVLAQRLARTICGSCKEPYHPTKEELDGLAYGYGEAAFAQLEIPG
                     KEDLLLYRGKGCTECNQSGYRGRIGLHELLVVSDEIKRLIHARATVAELWQVAVAQGM
                     T"
BASE COUNT          877 a          894 c          876 g          756 t
ORIGIN      
        1 cccccttgcc aaatcttaaa tcgctggagc gactttactg gagtgcttgt ggaaagagag
       61 cgttccccgt aggagaccgg tgattgtggc ggcccggatt cggaactaaa agaggtatag
      121 acttgggctg aggatttgct aatctagcac aaggcagctt tccggcggtt ccgtcgccac
      181 ggctcacatc ggtatttccg gttcccgatc gaacatcaaa agttgtcgct gcacaggaga
      241 gacgatggaa agtctcggag actatcaaaa ctgcaccatc ctatatgttg atgacgagga
      301 gaaatcgctc gtcaacttca accgagtctt tcgtaataag tttaagatac tgagcgccgc
      361 caatgccgca gagggctatc gcctccttga agaatatcga gacgatatcg cgcttctgat
      421 gagtgatcaa cgaatgcccg gggaaaaggg cgtcgaattt ttaaaacgcg ctcgcgaagt
      481 acatccaaac gcggtccgta ttctgactac cgcgtactct gatttcgatg tagcaattga
      541 ggcgatgaac tccggagccg tctccaaact cgtcaccaag ccgtgggata taccccagct
      601 ggagatgatc ttgaaggaag cctgtgattt ctttacagcg caacgcgaag gcgagtttct
      661 gccaggcacc gacttctctg cacaactgaa accgacgact cacgagcgag ggagtgagat
      721 aggtgagctc cggacgctgg agcaagtcgc ccgttactta aatgtcgaca agtttactgt
      781 ctaccgtctg atcacccaaa agaaaatacc ggcgttcaaa gtgggcaatc aatggcgttt
      841 caagcaggaa atgatcgacg cctggctgat ggcaaaagca aacatcaaaa agccgaaatc
      901 agattactaa gcaacgagtt cgctcgtaaa gtcctgtcgc cagttgccgg ccaccttagc
      961 tcgtaccagt tcaagcggtc gcaattcaat cttctgctct gtgaaggatc caattgatga
     1021 ttcacctcag tgtcccgtga ggtcgactac aaatcgtttc tcgtgtctgc gctcgttgcc
     1081 ttcaacaaat gagacgatgt catcggtaac agagtcctcc ttccgacctt accccctgcc
     1141 tctaaaatga tagcctgcca aacggtagct gatctcaacg ctccggcata gccaggtcat
     1201 cacatcaagg gcaaaagaca tgcaagtaca cggctagaca taaattgaca cttttgttct
     1261 tcgctggtca ccaggcgttt cttctgggct cgccaatgcg tctcttttcg cgatttttcc
     1321 ttggtatacc gcttgctcag gtcggctctc aaaccatgtc tcaacaattt gccgaaaaga
     1381 tcggtgacct cctcacgcag gaaggactac tcaccgcaga acaactcgcc aaaagcatcg
     1441 ccatacagaa acagcaagat tccacagagc cgattggcaa gatctgcgtt tcgctcggat
     1501 ttgtcgcgcc cacagatcta gaaaaagtat tgattaagca tcgccaccga attccgctgg
     1561 gggaactttt ggttcatctc ggactactca ctccgggcca agtacatagc gctctggaac
     1621 agcagaaaaa actgaacccg cataaaaagc tcggcgcggt actgattgaa aagggataca
     1681 tcgatgaggc tgcgctgatt cgcgcgcttt acgaacagtc tcaggcgggc aactgcgaaa
     1741 aactcaagca aggaaagttt gattcgctgg tagcgatgcg ccgtttgagc cagcgggatt
     1801 tagacctggc gcttcgggag gcgcagatcc agcagcggcc agtcgaaatt cttttgatgg
     1861 agcgctacaa gctaaaaaag caagaagtgg gcgccgctct cagcgtgttc tatggctgtc
     1921 cattcaaaga gtacgatgaa aaacagccgc ctgcctggga ttctatacgt ggcctcaatt
     1981 tgaattatct caaggccgct tcttggattc ctttaaaggt aagcgagcgc tccgtcgagg
     2041 tgctcatcga tgaccccaca gcttatgaaa aagtccagga catcaagcgg ctttttttag
     2101 gaaagcagat caagttcgcc gtaggcatgc gcgatgatat cctgagatat ttcagcacgg
     2161 ctaaggcgaa ccttgaaaaa ccccaatctc acgaatcgat cacgaccatt ctcgggcagc
     2221 tcgatgtcgc cgcacatgat gaagcagacg acgatagttt gaacgatccc gccatcaatg
     2281 agaatcacag cgccattgtc aggctggtga accagatcat tacggatgcc tgcaaaaaga
     2341 gggtttcgga tattcatatc gagccttacg gaagccaaaa tgacactgag gtgcgcttcc
     2401 gtgttgatgg gcactgctcc aattatctgc gcgtgccggc ggtgtatcgc agagctctcg
     2461 tttccaggct gaagattatg gcgggcctcg acatcgcgga acgccgcaag ccgcaagatg
     2521 gtaagatcaa gttccgcctg cccaatcgcg aaatcgaatt gcgcgtggcg actattccga
     2581 ctgcgggcat cggcaatgaa gatatagttc ttcgcgtgct cgcggccggg gagccgatcc
     2641 cgataaacga attacgcatg acgtctcgca atctaaacaa tttccgcgag ctgttggcaa
     2701 aaccgtacgg gttgattttg tgcgtcgggc caaccggatc aggaaaaaca accactcttc
     2761 attccgcgct taacgtcatc aatacagcgg agcgcaaaat atggacggca gaagatccag
     2821 tcgaaattac ccagcgcggg ctgcgccagg tgcaggtcaa ttctaaaata ggcttcaatt
     2881 tcgctgccgc aatgcgctcc ttcctccgag ccgatcccga tgtcatcatg gtcggagaaa
     2941 ttcgcgatcg cgagaccgcc gaaatcggta tcgaagcctc gctgaccggt catctggtac
     3001 tgagcactct tcacaccaac agcgccgtcg aaaccatcac tcgactctta gaaatgggga
     3061 tggatccgtt taattttgcc gatgctttgt taggagtctt ggcgcaacga ttggcgcgga
     3121 ccatctgcgg cagctgcaaa gagccgtatc atccgaccaa ggaagaactt gacggtttag
     3181 cgtacggcta tggtgaggcg gcttttgccc aactggaaat tcccgggaaa gaggatctcc
     3241 tcctataccg cggcaaaggc tgcactgaat gcaatcagag cggctacaga ggccgtattg
     3301 gtctgcatga gcttctggtt gtcagcgacg aaatcaagcg gctgattcac gcgcgcgcaa
     3361 ccgtagcgga actttggcaa gtcgcagttg cccagggaat gac
//
LOCUS       DASUTG010000327         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1534261, whole genome shotgun sequence.
ACCESSION   DASUTG010000327 DASUTG010000000
VERSION     DASUTG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534261"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VF089_07490"
     CDS             complement(<1..570)
                     /locus_tag="VF089_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6173843.1"
                     /translation="MKANCWYSKHDVRVEEVSEPKILNRRDAIVKITSTAICGSDLHL
                     YNGFIPTMEKGDILGHEFMGEVVEIGSEVKNLSTGDRVVASFPISCGNCFFCQKQMFS
                     LCENSNPNAPIAQKLFGHSPAGIYGYSHMLGGFAGGQAEYARVPYADVGTIKIPEGLA
                     DEKVLFLSDIFPTGYMAAENCEIQPGDTVA"
     gene            complement(592..1650)
                     /locus_tag="VF089_07495"
     CDS             complement(592..1650)
                     /locus_tag="VF089_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6173844.1"
                     /translation="MADTEYQPSEHDQSSLSRSRDSEYDNSRSPQRSGYGDLPGVSSN
                     GSQSLSRAEREEALAQALGWFSIGLGLAEIIAPRGLARLIGVSNNRTLLRMLGVRELA
                     SGIGILSRRRPVGWLWSRVGGDIMDLAVLGMALTSPTANRRRVAAATAAVTGVTVLDL
                     RASQQLSSQPELQDSAVSTQVKKSITINRSPEELYQFWHEFQNLPRFMNDLESVENIG
                     EKRSHWIVKGPAGKRIHWDAEITEDRPNELIAWRSLESSQVQNSGSVRFEPARGKPGT
                     VVRVEIDYRPPAGLLGATVAKFLGAEPKQQLHENLHRFRQLMETGEIITTEGQPAGRS
                     QSTSWKYDRAGRRLAASI"
     gene            2210..>2987
                     /locus_tag="VF089_07500"
     CDS             2210..>2987
                     /locus_tag="VF089_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX6173845.1"
                     /translation="MRIFLIHIRDPQFYALPATTRAANGNIRIMGFPPIGIMSLSAVL
                     KRAGHECVMFDQANPETPNPVIIEEIRRQQPSLIGLSFLSTTSYPYAKVLARQIRAMD
                     SRVRLAFGGVFATLNAHLVKTQCPEVDFICRGDGEQLILDLLDHLDDPAAVAGLTWAK
                     DGQVIDNPNRPLDRNLDQWPYPDRESLPLDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSPQHVIGEFEHLQENGYGAVYF"
BASE COUNT          650 a          827 c          802 g          708 t
ORIGIN      
        1 cgcaaccgta tcgcccggct ggatctcgca gttctccgcg gccatgtatc cggtggggaa
       61 aatgtcggaa agaaatagca ctttttcatc cgccaagcct tctggaatct tgatagtgcc
      121 gacgtctgcg tacggcaccc gcgcgtattc cgcctgcccg ccagcaaagc cgccaagcat
      181 atgcgaatag ccataaatcc cggcggggga atggccgaat aatttctgcg cgatgggggc
      241 attcgggttg gagttctcac aaagagagaa catttgtttt tgacagaaaa agcagttgcc
      301 gcaggatatc ggaaaggaag ccacaacccg atcgccggtg ctcaagtttt tgacttcgct
      361 gccgatctca accacctccc ccatgaactc atggccgagg atatcgccct tttccatcgt
      421 cggaatgaat ccgttgtaca aatgtaagtc ggagccgcag atcgccgttg aggttatttt
      481 tacaatcgcg tcacgacggt taaggatctt tggttcgctc acttcttcaa cgcgcacatc
      541 gtgcttgctg taccagcaat tagctttcat cgaatcctcc tgttagatgg gctaaatcga
      601 agcggcaaga cggcgtccgg cccgatcata tttccaggaa gtgctttgtg agcgccccgc
      661 cggttgacct tctgtggtaa tgatctcgcc ggtttccata agctgcctga atcgatgcag
      721 gttctcatgc aactgctgtt taggttctgc gcctaggaat tttgcaaccg tagcgccgag
      781 caaaccggct ggcggacggt aatcaatctc cacacgaaca acggtgcctg gtttacctcg
      841 tgccggctca aaccgcaccg accccgaatt ctgcacctga gaactttcta acgagcgcca
      901 ggcgatgagc tcgttgggcc gatcttccgt aatctcggcg tcccaatgaa tccgtttgcc
      961 agccgggccc tttacgatcc agtgcgaacg cttttccccg atgttctcta cggattcgag
     1021 atcattcata aatcggggca aattctgaaa ttcatgccag aattgataaa gctcttcggg
     1081 agatcggttg atggtgatgc tcttttttac ctgagtagaa acggcgctgt cctgcagttc
     1141 cggctgactg ctaagttgct ggctggcgcg caggtcaaga accgtgacgc cagtaacggc
     1201 ggcggtcgcc gccgcaactc tgcggcgatt tgcagtcggc gaagtaagcg ccatccccag
     1261 aaccgccaaa tccatgatgt cgccgccaac ccttgaccaa agccagccca ctggcctgcg
     1321 gcggctaagg attccgatgc cgctggctaa ttcgcgaact ccaagcatac gcagcaaggt
     1381 gcggttatta ctgacaccga ttagccttgc tagccctcgg ggagcgatga tctcggcaag
     1441 gccgaggccg atgctgaacc atccaagcgc ttgggcaagt gcttcttccc gttccgctct
     1501 cgataggctt tggctgccgt tactgctaac gccgggaaga tcgccataac cgctacgttg
     1561 ggggcttcta ctattgtcgt attcactgtc gcgcgatcgg ctgagcgaac tttggtcgtg
     1621 ttcggatggt tgatattccg tatccgccat ggcttcctcc agaaattaaa ttttccagct
     1681 agcccatgta gctctcgatt tagagaggct ggactgtcgg cgcaagcacc atactgctgc
     1741 tgtcttggga gcggtagcag ttggcgcaaa cgatgtgcca ttggcagctc gtgttgctcg
     1801 aagctgatta tgaagaatgt aattgaaaat acacgaccaa ctccgatgcc caaagcagcc
     1861 ggctcaagta ctgcggcgaa tataggacaa tcagctagcg gcgacttgcc acttgtggga
     1921 cgatcgatgc acgagataat aacgatgcag cttggttgcc gtcgagagga cgaccactgg
     1981 atcgggctcg caaatggcgc tggttttacc gtgcggaatg tacgaggatc cacagatgaa
     2041 ttaatcctcc ggatttcggt tgtggatgtg gctgccgttt gttaaggtca tgactgtcgt
     2101 tgaagttctt aaaccgctat tatcgtcaag tctaggattg tcactaatca ccaagtcaga
     2161 tctcgctcca aacgggaggc aagagccgac gggaacgagt aagtaatcga tgcgcatatt
     2221 tctgattcac attcgcgatc cgcaatttta tgccctgccg gctacgaccc gcgccgccaa
     2281 cgggaatatc agaatcatgg gttttccgcc gatcggcatc atgtcgcttt ctgcggttct
     2341 caaacgcgcc ggacacgaat gcgttatgtt cgatcaggcg aacccggaaa cgcctaaccc
     2401 ggtcatcata gaggaaattc gccggcagca gccgagtcta atcggattga gttttctaag
     2461 cacgactagc tacccgtacg ctaaagttct tgcgcgtcaa atccgggcca tggattcacg
     2521 ggttcgattg gcctttggcg gcgtgttcgc gaccttaaat gcccatttag tcaagacgca
     2581 gtgtcccgag gtagatttta tttgccgcgg cgatggcgaa cagctcatcc tcgacctgct
     2641 tgaccatctg gacgatcccg ccgcggttgc cggcctcact tgggccaagg acggccaggt
     2701 catcgacaat ccaaatcggc cgttggaccg caatctcgat cagtggccgt atcccgatcg
     2761 cgagagcttg ccgctggact ttgtcgaatc gatgccgctc gatgttcccg ccgtgttgtc
     2821 gatggaacgc ttcacgacga tgcaaacctc gcgtggctgc ccctggccct gcgttttttg
     2881 cgacattccg atctttaacg aaggtaagtg gcgcgcgcgc agcccgcaac atgtcatcgg
     2941 cgagttcgag catttgcaag aaaatggata cggcgccgtg tatttcg
//
LOCUS       DASUTG010000328         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1979085, whole genome shotgun sequence.
ACCESSION   DASUTG010000328 DASUTG010000000
VERSION     DASUTG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979085"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF089_07505"
     CDS             <1..736
                     /locus_tag="VF089_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6173846.1"
                     /translation="PPILKQLCEREKGLILVTGPTGSGKSTTLAAMIDYLNNGFEGHI
                     LTVEDPIEFVHPSKKCLVNQRELGPHTHSFANALRAALREDPDIILVGELRDLETIQL
                     ALTAAETGHLVFATLHTSSAPKTVDRVIDVFPPNQQAQIRAQFAESIEAVITQTLLKK
                     KGASGRVPALEIMIGTTAVRNLIREGKIHQLPGTMQVSKKDGMQTMDMALLDIVARGL
                     VTKEEAQTRSSNPNLFGAASAASVAR"
     gene            774..1946
                     /locus_tag="VF089_07510"
     CDS             774..1946
                     /locus_tag="VF089_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6173847.1"
                     /translation="MNIEEYFRAMVEKDSSDLYLTVARPPMYRVNGKVRASDNESFTP
                     ESLEDLAKSFINEEQWEEFRQKKELNLALSLPRISRFRVNIMRQRGSIAIVVRKIKVD
                     IPSFDSLGLPPVLKEVAMLKRGLVLVVGATGSGKSTTLASMIDLRNSNDEGHIITVED
                     PIEFVHKHKNSVVTQREVGFDTNSFQSALENAMRQAPDVILIGEIRTSETMEAAITFA
                     DTGHLCFGTLHSNNANQSFERILNFFPPERYSQVLLQLSLNLRAIVSQRLLPAVGGGR
                     VAAIEILLDTPRMKDLIKKGEIDTLKEAMEQGIREGCQTFDQALLVLYQAGKITIGEA
                     LANADSANNLRLKIKMAGLVADETAEGNTLLSNPENGAEPKADGVGSNNVFRLQGR"
     gene            2069..3250
                     /locus_tag="VF089_07515"
     CDS             2069..3250
                     /locus_tag="VF089_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6173848.1"
                     /translation="MMEIKQLLQVMVEKEASDVYLTVGSPPMYRIEGIVQPIGDVEFA
                     PNDLEKLAESVMSEKQWQEFNSKLEMDLVLVLSIPDLSRFRVNVLRQRGFVALVIRKI
                     RFEIRSVDELKLPQILKDIVMRKAGLVLVVGATGSGKSTSLAAMIDHRNSSAPGHIIT
                     VEDPIEFVHRHKRCIVTQRELGFDTHSFGDALKRAVRQAPDVILIGEVRDRDSMEAAI
                     TFADTGHLCLGTLHSTNVNQSFERILNFFPAERHSQILMHLSLSLRAVISQRLVPSVD
                     GARVAAIEILIDTPRIKDLIKQGDIDSLKEAMEQGIQEGCQTFDQALFTLYKENKITL
                     QQALMNADSANNLRLKIKLEGLIGDDALDKLLEKGSASSNKRGLQIQGNQRIGSTFRR
                     I"
BASE COUNT          909 a          849 c          962 g          841 t
ORIGIN      
        1 gcctcccata ctaaaacaac tctgtgaaag ggagaaagga ctgatcctag tcacgggacc
       61 taccgggtcc gggaaatcca cgactctggc tgcgatgatc gactacctga ataacggctt
      121 tgaaggtcat attcttactg tcgaggatcc tatcgagttt gtgcacccat ccaagaaatg
      181 tcttgtgaac cagcgcgaat tgggtcctca tactcattcc ttcgctaacg ctctaagagc
      241 ggcgctccgt gaagatcctg acatcatcct agtcggagaa ttgcgcgacc ttgaaactat
      301 tcaactcgcg ctgactgctg ccgaaaccgg tcatttggta tttgccactc tgcacacctc
      361 cagcgcgccc aaaaccgtcg atcgagtcat cgacgtgttt ccgcccaacc agcaagctca
      421 aatccgcgca cagtttgcag agtcgatcga ggcggtgatc acacaaacct tgttgaagaa
      481 gaaaggcgca agcggcaggg tgccagcctt ggagattatg atcggcacca ccgcagtccg
      541 caaccttatt cgggagggta aaatccatca gttaccgggg acgatgcagg tgagcaaaaa
      601 agacggcatg cagacgatgg atatggcgct gctggacatt gtggcgcgcg gcctcgtgac
      661 caaggaagaa gctcaaacgc gcagttcgaa tccgaatctt ttcggtgccg ccagcgcggc
      721 ctctgtggca cgctgagctc aactgacaaa ccacaacggc gaggtgagga ttgttgaata
      781 ttgaagagta ttttagggcg atggtggaga aagattcctc tgatctttac cttacggtgg
      841 ctcggcctcc gatgtatcgg gtcaacggta aggtccgagc gagcgataac gaatccttta
      901 cgcctgaatc tctcgaagat ctggcaaaat cgttcatcaa tgaggaacag tgggaggaat
      961 ttaggcagaa gaaagaactg aatctagcgc tttctttgcc gagaatcagc cgttttcgcg
     1021 tcaatattat gcgccaacgt ggctcgatcg caatcgtggt tcggaaaata aaggttgaca
     1081 tcccatcctt tgactcgctc ggactaccgc cggtgttgaa agaagtagcg atgttgaaaa
     1141 gaggtctagt gctggttgtc ggcgcgacag ggtcgggtaa gtcgaccacc ttggcatcga
     1201 tgatagatct tcgcaacagc aatgatgagg gacatatcat tacggtggaa gatccgatcg
     1261 agtttgttca taagcataaa aattccgtag tgactcagcg cgaggtcggc tttgacacta
     1321 actcattcca gagcgccttg gaaaatgcta tgcgacaagc gccggacgtc atcctaattg
     1381 gggagattcg tacctccgag accatggaag ccgcgataac tttcgccgat accggtcatc
     1441 tttgtttcgg aactttgcat tcaaacaacg ctaaccagtc attcgaacgg atactcaatt
     1501 tttttccgcc ggagcgatac tcgcaggtgc tcttgcagct gtccttgaat ttgcgggcta
     1561 tcgtatctca acggcttctg cccgcggtcg gcggtggacg ggttgcagca attgaaattc
     1621 ttttggatac accgcggatg aaggacttaa taaagaaggg cgagattgac accttaaagg
     1681 aggcgatgga gcaggggatt cgggaaggtt gtcaaacatt cgaccaagcg ttgctcgttc
     1741 tatatcaagc gggaaagatt accatcgggg aagctttggc taatgcagat agcgccaata
     1801 atctacggtt gaagattaag atggccgggt tagttgccga tgagacagcc gaagggaaca
     1861 cgctgctgtc aaatccggaa aacggcgcag aaccaaaagc ggacggtgtc ggcagtaaca
     1921 atgtatttcg cctccagggc agatagcgac acaaggttca acgaataaat accggcgata
     1981 gcgaatcggg gcctcataca agtcggatca ttatcgccct gtatgtaacg atcactcgac
     2041 aggagttaga agctaccttc ggaggaagat gatggagatt aagcaattac ttcaggtgat
     2101 ggtggagaag gaggcttctg acgtttatct cactgttggc agtcctccta tgtaccgcat
     2161 tgagggtatc gtccaaccta tcggggacgt cgaatttgcg ccgaacgatc tggagaagct
     2221 tgcggagtcg gtgatgagcg aaaaacagtg gcaggagttt aactcgaaac tagagatgga
     2281 tttggtgctt gttctttcaa tacctgatct cagccgcttc cgtgtcaacg ttctgcggca
     2341 gcgtgggttc gtggcgctgg ttattcgcaa aatcagattt gagatcagat ctgtcgacga
     2401 actgaagctg ccgcagatac taaaagatat cgtcatgaga aaggcgggct tggttctggt
     2461 ggttggtgcc accggatccg gtaagtccac cagtctggcg gccatgatcg atcatcgtaa
     2521 ttcatccgct ccgggccata tcatcacagt ggaggatccc atcgaattcg ttcatcgaca
     2581 caagcgatgc atcgtaaccc agagagaatt agggtttgac acgcactcct ttggcgatgc
     2641 gctcaagcgt gcagttcggc aagcgcccga tgttatcctc atcggtgagg tccgcgacag
     2701 agactcgatg gaggcggcga ttactttcgc cgataccggt catctttgct tggggacgct
     2761 tcattcaacc aacgtcaatc aatcgttcga acgtatcctc aatttctttc ccgctgaacg
     2821 gcactcacag attctcatgc atctttcttt gagtttacgt gccgtcattt cgcagcgttt
     2881 ggttccttcg gtggatggag cgcgggtggc ggccatcgag atcctgattg atactccacg
     2941 gattaaagat ctaatcaagc agggagacat cgatagcctt aaagaggcta tggaacaggg
     3001 aattcaggag ggttgccaaa cctttgacca ggcgctgttc actttgtaca aagagaacaa
     3061 aatcacgctc cagcaagcct tgatgaatgc cgatagcgct aataacctgc gccttaagat
     3121 caaactggag ggtttaatag gcgacgatgc cctggacaag ttgctagaaa agggaagtgc
     3181 ctcctccaac aagcgggggt tgcagattca aggtaatcaa cgaatcggca gtactttccg
     3241 gaggatttag tgcccgtggt gctatcgcta taaactaaat ctttcttgat ttaagatgag
     3301 gcgacgcggt agctaagccg tttttggctg cttggaactg tagagcagga ctatcgaaca
     3361 gacatcatag ctgttttgag gtcgcgaagc tgcagagctc gagtatcgct atcgacgact
     3421 aagcggccgt tgactcgaaa gcaagcaatt ctctgtaggt caatgacaag gagaaatcat
     3481 acttagtacc catggctgat caaaccgttt acgctctttt acttcgccct gtggggcaat
     3541 tgctcgaggc gctgctgatc g
//
LOCUS       DASUTG010000329         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_349134, whole genome shotgun sequence.
ACCESSION   DASUTG010000329 DASUTG010000000
VERSION     DASUTG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349134"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..449)
                     /locus_tag="VF089_07520"
     CDS             complement(18..449)
                     /locus_tag="VF089_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6173849.1"
                     /translation="MSSVLSVITIACQAQPKVTIETANGAISFQVEIAATPEKREVGL
                     MYRRELPPNYGMLFIFPGERVNSFWMKNTPIPLDMIFISRERKIVGIVHETAPFSLDG
                     RSVSVPSQYVLEINGGLSRRYGFKAGDVVRFDNVMPESVRE"
     gene            complement(487..2481)
                     /locus_tag="VF089_07525"
     CDS             complement(487..2481)
                     /locus_tag="VF089_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6173850.1"
                     /translation="MLTSLEKILFLLFLAVSLYYGGRRFYAVYRSIARGKPDGRFDQL
                     PRRVIDALVNVVLQRSVFRMRPVVSFFHALVFYGFVFYFLVNLVDVVEGYIALHVRGG
                     LWNAFNLAADILTAAVLIGIISLVIRRFFVRPKDFDFPANVPVREGVREGIRRDSTIV
                     STFILFHVGSRLMYKASQVALEGADPWQPVSTAIAALFSGMTPGALEIFHHFFWWGAL
                     GSILAFIPYFPRTKHIHILMAPINLALRKKNPGVLQPMDFENEEVFGASKLEEFAWPR
                     LMDAYSCIMCNRCQDVCPATNTGKALSPAAILINERFELNDLFPRFAAGVESPRPLLD
                     FALNEEAAWACTTCNACIEVCPVGNEQMLHIMDVRRERVLSAAEFPNGLKNTFNQMET
                     AGNPWGISAEERLVWAKDLPFPVPTVDQNGNPDVLYWVGCAVSFDPRAQQIARNMAEI
                     LHSAGLNWAVLGKTEKCTGDTARRTGNEYLFAQMADENIAALNAVNAKVIITTCPHCF
                     HTIGKEYPQFGGNYTVKHHTEFISELIRDGKLKIEPNSATAITYHDPCYLGRHNGIFD
                     QPRSLIEMTGANLIEPERTGNNSFCCGAGGGQFWKDEERGKERVSTNRFRELKQTGAQ
                     TVATGCPFCLRMLTEETAKEDGSGPEVFDIAELVTTKIRK"
     gene            complement(2689..3468)
                     /locus_tag="VF089_07530"
     CDS             complement(2689..3468)
                     /locus_tag="VF089_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX6173851.1"
                     /translation="MVRREKSNYIIQSVSHALDVLEQFNGNVDEIGVTELSKRLKLHK
                     NNVFRLLATLEARGYIEQNKVSENYRLGLKCLQLGQTFIHQMGLLLQARATLEELTQS
                     ANESAFVAIRKGAGIVPLDFVEPARPVRVVSFLGSVLPSHCTAVGKVYLAFESDGGVS
                     LGDLDPLKRYTDKTITDTRGLAQQVKETGEIGYAIDDGEFINEISSIAVPVRDYTRSL
                     VGALAVVGPSDRLTLEKLRNDISPLILDAGRDLSRRLGYAG"
     gene            3871..>5322
                     /gene="trpE"
                     /locus_tag="VF089_07535"
     CDS             3871..>5322
                     /gene="trpE"
                     /locus_tag="VF089_07535"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX6173852.1"
                     /translation="MIRPTFNEFCELAEQGNLVPVYQELLMDLETPLSFFKRLERDRY
                     AFLLESVEGSERWARYSFLGSQPRLVFKSRGKQVEIIEDGKSRKLDSEMPLDSLKELV
                     GNYRPVTVPGVPPFFGGALGYVAYDAVEQFHGISNNKNPPFDAPEIFFLFVQTLIAFD
                     NLKHTIKVIDNVHLEPGVNLRTAYDQATDKIRKIISSLQKKPRGIEPRELGDGKAQKK
                     FRSNLNRDGFQNAVIKAKEYIQAGDIIQTVLSQRLETETVADAFEIYRALRFINPSPY
                     MFYLELEDLRIIGSSPETMVRLTGDTVELRPIAGTRPRGATPEEEQTLEADLLGDPKE
                     RAEHIMLVDLGRNDIGRIAEIGSVEVNELMGIERYSHVIHIVSNVRGKLAADKSPFDL
                     FVSAFPAGTVSGAPKIRAMQIISELEPEKRGLYAGAIGYFGYNGNLDTCIVIRTIVMK
                     GSKVFITAGAGIVADSDPDAEYQETLSKARAMFR"
BASE COUNT         1290 a         1490 c         1358 g         1184 t
ORIGIN      
        1 cgcggctcgg cgccgttcta ttccctcacg ctctccggca tcacattatc aaacctgacc
       61 acgtccccgg ctttgaatcc gtaccggcgt gaaagcccgc cgttgatttc gaggacgtac
      121 tgactaggga ccgacaccga acgaccatcc aaagaaaacg gcgcggtttc atggacgatg
      181 ccgacgattt ttcgctcgcg actaataaaa atcatgtcca gaggaatcgg cgtgttcttc
      241 atccagaaag aatttactct ctcacctgga aagataaaca acatcccgta atttggcggc
      301 agctcgcgcc gatacatcag gccgacctcg cgcttctctg gagtggcggc gatctcgacc
      361 tgaaagctga tcgcgccatt ggcggtttca atggtcactt tgggttgggc ctggcaagct
      421 attgtaatca ctgaaagaac tgagctcaaa gctaaagctc cagcatcaat tctccaagac
      481 agtgccctat ttacggattt tcgttgtgac cagttccgca atatcgaaca cctccggccc
      541 gctgccgtct tctttggctg tctcctccgt aagcattcgc aagcaaaatg gacaaccggt
      601 cgccacagtt tgcgcgccgg tttgcttgag ctcacgaaac cggttggtac tcaccctctc
      661 cttgcctctc tcctcatctt tccagaactg tccgccaccg gcgccgcaac aaaagctgtt
      721 gttaccagtg cgctcaggct caatgagatt ggccccggtc atctcgatca gcgaacgagg
      781 ttgatcgaaa atcccattgt gccggccgag atagcacgga tcgtgataag ttatcgcggt
      841 ggcagagttc ggctcgatct tgagcttgcc atcgcgaatc aactcgctaa tgaactcggt
      901 gtgatgtttt actgtgtagt tgccgccgaa ctgcgggtat tccttaccga ttgtatgaaa
      961 gcagtgggga caggtcgtga tgatgacttt ggcgttgaca gcattcaacg cggcaatatt
     1021 ctcgtcggcc atctgggcaa agagatattc attaccggtg cgccgagcgg tgtcgcccgt
     1081 gcatttttca gtcttgccta gtaccgccca gtttaaaccg gcggaatgga gaatctccgc
     1141 catgttgcgc gcgatctgct gcgcgcgcgg gtcgaacgag actgcgcagc cgacccaata
     1201 aagcacgtca gggttgccat tctgatccac cgtcggcacc gggaacggca agtccttcgc
     1261 ccagactagc cgctcctccg ccgaaatgcc ccaagggttt cctgccgtct ccatctgatt
     1321 gaacgtattt ttaaggccat tcggaaactc ggcggcgctc aacacgcgtt cacgcctgac
     1381 gtccatgata tgcagcatct gctcattgcc caccgggcaa acctcgatac aagcattgca
     1441 ggtggtgcaa gcccaagccg cctcttcatt gagagcaaaa tcgagtaacg gccgcgggct
     1501 ctcgaccccc gccgcgaacc tcgggaaaag atcgtttagc tcgaatcgtt cgttgatcaa
     1561 tatcgctgct gggcttagcg ctttgccggt gttggtcgcc ggacagacat cctgacagcg
     1621 gttacacatg atgcagctgt aagcatccat cagacgcggc cacgcgaact cctccaattt
     1681 ggacgcgccg aacacctcct cattctcaaa atccatgggc tgcaacacac ccggattctt
     1741 tttcctaagg gctaaattga tcggcgccat caatatatga atgtgtttgg tgcgcgggaa
     1801 gtacggaata aaagccagga tcgaccctaa tgcgccccac caaaagaaat gatggaagat
     1861 ctcgagtgcg cctggagtca tcccggaaaa tagagccgca attgcggtgc tcaccggttg
     1921 ccacgggtcc gcgccttcaa gcgcaacctg cgacgctttg tacatcaacc ggctgcccac
     1981 atgaaaaagg ataaaagtcg acacaatcgt cgaatcgcgg cgaatgcctt cgcgtacacc
     2041 ctctcgtacc ggcacgttcg cgggaaaatc gaaatcttta gggcggacaa aaaaacgccg
     2101 gattaccaga cttataatgc cgatcaacac agccgccgtg aggatatcgg cggccagatt
     2161 aaatgcattc cacaaaccgc cgcggacgtg caaggctata tagccctcga ccacatcgac
     2221 taaattgacc aagaaataga aaacgaagcc gtagaacacc aatgcgtgaa agaaagaaac
     2281 caccggtcgc atccgaaaga cggagcgctg aagaacaaca ttcaccagag cgtcgatgac
     2341 ccgcctgggt agctgatcga accggccatc cggcttgcct cgagcaatgc ttcgatacac
     2401 ggcgtaaaat cttcgcccgc cgtaatagag cgataccgcc aagaacagaa ggaaaagtat
     2461 cttttcgagc gacgtaagca ttagtgcttt atcaacctcc ggcctgtcta ctttgtccaa
     2521 ttgattgaag ctgatgccct tttaacgcgg caccggtaac gatccggaat gcagcgaatc
     2581 agtcgcaacc gcgtcgagta aataagttga ctctacagga agcagtattt atgaatcaac
     2641 gggcggtgtt tctaaggagc tgtcaagtag atctatcgac tcagcgcatc acccggcgta
     2701 ccccaaccgc cttgaaagat cgcgtccggc atcaagaatc agaggcgaga tgtcgtttct
     2761 gagcttctcc agagtaagtc gatcactcgg tccgactacc gcaagcgcgc cgaccaggct
     2821 cctagtgtag tctctcacgg gaacagcaat cgaactgatc tcgttaataa actccccatc
     2881 gtcgatggcg taaccgatct ctccggtttc tttgacctgc tgggcgagcc ctctcgtatc
     2941 ggtaatcgtc ttatcggtgt aacgcttcaa agggtcgaga tcgccaagcg acacaccgcc
     3001 atccgattca aaagcaaggt ataccttacc gacggcggtg caatgcgatg gcaacaccga
     3061 gccgagaaac gagacaactc gcacgggccg cgccggttcg acgaagtcca gcggcacgat
     3121 tcccgcccct tttctaatcg ccacgaacgc actctcgtta gctgattgag tcagctcttc
     3181 cagagttgcg cgggcctgca acaacaaacc catctgatga atgaaggtct gccccagctg
     3241 cagacacttg agcccgaggc gataattttc cgagactttg ttctgttcaa tgtaaccccg
     3301 tgcctcgagc gtggccaaca gacgaaaaac attgttcttg tgaagcttca gtcgcttgct
     3361 caattcggtg acaccgatct catcaacatt tccgttgaac tgctccagaa catcaagggc
     3421 gtgtgataca gattggatta tgtaattaga tttctcccga cgaaccatgt ctcctcctca
     3481 atttacgttc acgggtgatc ccgaataggc tagctcgggc cgttccaaaa aagcgtttta
     3541 acctataccg ccagccggac cttgtcaacg aaaccacaaa gggtttagat ggctcaacga
     3601 caggaccatc ggagcacctg atactccgca cgccggatca tgatgtctgt aaagaactcg
     3661 acaccatctc tctaatcgcc ttgaccagta taagacaata aggcacactt cgtcccatga
     3721 ctgagcggtc gggatttgct gccttgattg agagttggtg gtgcagccag cagtcgctcc
     3781 gatcggcctg tggctaattg cagtcagtca actgaacagt gaggagggcc gtaggcgatt
     3841 agcttatggc cctttttgtt tgtgagactt atgattcgtc cgacgtttaa cgaattttgc
     3901 gagttggcgg aacaagggaa cctggttcca gtctatcagg aattgctcat ggacttggaa
     3961 acaccgctct ctttttttaa acgccttgaa cgggatcgct acgcgtttct gctcgagagc
     4021 gttgagggga gcgaacgttg ggcgcgttac tcgttcttgg ggagccagcc tcggctggtt
     4081 tttaaaagcc gaggcaagca ggtcgaaatc atcgaagatg gtaagagcag aaagctcgac
     4141 tccgaaatgc cgcttgattc tctgaaagag cttgtgggga attatcgacc ggttacggtt
     4201 ccgggtgtcc cgcctttctt cggtggcgcc ctaggctatg tcgcctatga tgcggttgaa
     4261 caatttcacg gcatcagcaa taacaagaac cctccgttcg atgcgccgga gatttttttt
     4321 ctgttcgtcc aaactctgat cgccttcgat aacttaaaac ataccattaa ggtcatcgac
     4381 aacgtccatt tagaaccggg agtcaatctt cgaaccgcgt acgatcaagc taccgacaaa
     4441 atccgcaaaa ttatctcctc cctgcagaag aaaccacgag gaatcgagcc tcgtgagctg
     4501 ggcgatggca aagcgcagaa gaagtttcgc tccaatttga atcgggacgg ttttcaaaac
     4561 gcggtcatca aggcaaagga atacattcag gcaggcgata tcattcaaac agtgctatct
     4621 cagaggctcg agaccgaaac ggttgcggac gccttcgaga tttaccgcgc tctgcgcttt
     4681 atcaatcctt caccgtacat gttttatctc gagctcgagg acttacggat catcggctcg
     4741 tcgcccgaga cgatggtgcg actgaccggc gatacggtcg agctccggcc gattgccgga
     4801 acccggccgc gcggcgccac accggaagaa gaacagacgc ttgaagccga cctgcttggc
     4861 gaccctaaag aacgggctga gcacatcatg ttggtcgatc ttggacgaaa cgatatcggg
     4921 cgtatcgctg aaatcggcag cgttgaagtc aacgagctca tgggcatcga acgctactct
     4981 catgtcattc acatcgtttc caacgtgcgc ggcaagctcg cagccgacaa gagcccgttt
     5041 gatctatttg tctcggcctt ccctgccgga acggtttctg gagcgccgaa gattcgcgcc
     5101 atgcagatca tcagcgagct ggagccggag aaacgcgggt tgtatgccgg cgccatcgga
     5161 tacttcggct ataacggcaa tctcgatacc tgcatcgtca ttcgaacgat tgtgatgaaa
     5221 ggcagcaagg tattcatcac cgccggagct ggaattgtcg ccgactcgga tcccgacgcg
     5281 gagtatcagg agacgctgag taaggcgcgg gcgatgtttc gc
//
LOCUS       DASUTG010000330         4699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1979182, whole genome shotgun sequence.
ACCESSION   DASUTG010000330 DASUTG010000000
VERSION     DASUTG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979182"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VF089_07540"
     CDS             <1..576
                     /locus_tag="VF089_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6173853.1"
                     /translation="YENNSGMRAARALWFLDYFGHSSARMLDGGMQAWKAAGFPTTTE
                     VISPKGAAFKISERREVLATADDVLQSLNKKGVSILDTRSRAEYVGRQIRAARGGAIP
                     GAIHIEWTDNLDTSGKFKTDAELEDMYSRAGITADKEVISYCQGGYRAAHSYVALRLI
                     GFPKIRNYIGSWKEWGDRADLPIDQPAERSE"
     gene            complement(612..1181)
                     /locus_tag="VF089_07545"
     CDS             complement(612..1181)
                     /locus_tag="VF089_07545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6173854.1"
                     /translation="MSGVPAENFAAAPWSERPSVRTFREQLAPFDPTPHEVVDRMLAL
                     GGIKPGDLLYDLGSGDGRVLIAAAKKYRIKAVGFEVDPGLVKLARENIKQENLEQLVE
                     VRHQDFMTADLSSASIVTLYLSHDGNQALKPILLHQLQPGARIISYTFDMGDWPPKIT
                     ESYRDGAGNVHTLYYWEIPQSLAHAEKSH"
     gene            complement(1715..2587)
                     /locus_tag="VF089_07550"
     CDS             complement(1715..2587)
                     /locus_tag="VF089_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6173855.1"
                     /translation="MSGMKRCPNCSTEYDDNTSFCTKDGRALVIATTNRARLCPHCAN
                     SIEEDAVRCPYCKAEFGSTEERPQWPSRDEMAGQPARTRGQPGRRAWTKRAMATLGAS
                     LIIVLGFGLYLIAGYGQRNDTRRLEEANSKQVQEREEKIKALEGELRQLRQELDKSKN
                     ELTSLAAKLEENQKELSATQQRLTGATREAAAVAAKRPAIAPGASARSVKTPPSQTPA
                     ARRSAEPGVYEVIRATTVREEPSITARRITEINKGTKITVVRTVGSWLEVRSKQGRPP
                     GYVRLDDAILVTRR"
     gene            2882..3820
                     /locus_tag="VF089_07555"
     CDS             2882..3820
                     /locus_tag="VF089_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6173856.1"
                     /translation="MALLIGEPQTRKLINMARAVDLLDKMFLDRAAGKIRGLPRRRLK
                     GNEKQINIMAAWHQGYDLFCLRAYIGQANTITLYNGKSGQIQAIINMGYLSSLRTGAA
                     TGVAAKYLAPRNASTLGVIGPGWQATFQVEAVAHACRIERIVVFGRTPKRRKQFIKQM
                     SQIVKAVWHEASSVEEVERESDILVVSTNSTTPVADGKNLKTEALVASIGANAAIKHE
                     VSGNLIGQMDLIIVDDVAAARTDSGDLIDACQNGIARWEDVLPLEKFAGSGPSQPRPK
                     RIFFQSNGIADEDLAVGRYVLEQAKRKLKLRQVTEI"
     gene            complement(3965..>4699)
                     /locus_tag="VF089_07560"
     CDS             complement(3965..>4699)
                     /locus_tag="VF089_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173857.1"
                     /translation="GGVMLNVLSQRVFVIGLLSILTLAGCATTQSQQDFSSILADKER
                     PDTERALDSTRKPNEVLAFYGVKSGDKVADVFAGRGYYTAILSQVVGPRGMVYSVVPA
                     PRPELTERFRNPKFANVRIVEGPLDKLALPQDGSLDFALIHLNYHDLSPDVRIAMNKR
                     INGALKPGGVYGVVDHSAKEGSGNEAVKTLHRIDKLLVIKEVTGSGFKLAKEGTILSR
                     PEDKRDFNVNKQRNLSDRFVLAFQKP"
BASE COUNT         1080 a         1295 c         1263 g         1061 t
ORIGIN      
        1 tatgagaata actccgggat gcgcgccgca cgggcgctct ggttcctaga ttacttcggc
       61 cattccagtg cgcgcatgct cgacggcggc atgcaggcat ggaaagccgc gggatttccg
      121 acgactactg aagtaatctc ccctaagggc gccgctttca aaatcagcga acgccgcgaa
      181 gtgctggcta cggcagacga cgtccttcaa tcgctcaaca aaaaaggcgt ttcaatcctc
      241 gacactcgca gccgcgccga atacgtcggc cggcagattc gagcggcccg aggcggagcc
      301 attcctggcg cgattcatat tgaatggacg gataacctgg ataccagcgg caaattcaaa
      361 accgatgccg agctggaaga catgtacagc cgggccggca taacggcgga caaagaagtg
      421 atttcgtact gccagggagg gtatcgggcg gcgcacagtt acgtggcgct gcggttaatc
      481 ggttttccca aaatccgcaa ctacatcggt tcctggaaag aatggggtga ccgcgccgat
      541 ctgccgatcg accaaccggc ggagagaagt gaataagcga ctaagcgcag aatgagatag
      601 gaaaggccca tttaatgcga cttttcagca tgggctagcg attgcggtat ttcccaatag
      661 tacaacgtgt gcacgttgcc cgctccatca cgataggact ccgtaatctt cggcggccag
      721 tcgcccatgt cgaaggtgta cgaaataatc cgcgcgccgg gctggagttg atgcagtaag
      781 atcggcttga gcgcttgatt tccgtcatga gagagataca aggtcactat gctggctgac
      841 gacaagtcgg ccgtcatgaa atcctgatgc cggacttcca ccagctgctc caagttttct
      901 tgcttgatat tctcacgcgc cagttttacc aatcccggat ccacttcaaa ccctactgct
      961 ttgattcgat atttttttgc tgcggcgatc agcacccggc catcgccgga accaagatca
     1021 tacaggagat cgcccggctt tattccgccg agcgctaaca tccgatccac aacttcgtgc
     1081 ggcgtgggat cgaaaggcgc aagttgttcc cgaaacgttc ttaccgaagg cctctccgac
     1141 catggggccg cagcgaaatt ctccgccgga acgccggaca cgatggagcc agttgcggct
     1201 gaatcagatg agctgggaac ctcgatgatt gatcctccag ggttgtacga gaagtaaccg
     1261 tatcggtccg agtacgaaag atacggatgc cagtgataat agataaatgg cggatacaat
     1321 cgtttgacaa ttactcgccc cggaaaggta aactggataa atggtcggcg tcgaaagtga
     1381 gcgcgaccga taagcggacg cggaatagaa cttcgatgcc cggtgaggga accatccagc
     1441 cggcccaatc tcggccttac ggcagaggat ccggacttaa aataaccgct caggttatgg
     1501 ccagctccac gatgcccccg aatcctttgg ctccaagcct gatccggcgc caataagaat
     1561 atggcgaaaa taaacgacaa aatgtgcatc ctgtcgaatc ttaggagcac tcgttagcgt
     1621 aagtcaacta gctgaaagaa gcgcgggttt gcctcaaacc aaaaccgaac tttcgagacg
     1681 gccttcactc cgaagtatcg aggcaatgct attcctacct tcgagtgacg agtattgcat
     1741 catccaaacg aacatagcct ggcggtcttc cttgctttga gcgaacttcg agccagctgc
     1801 ccaccgtgcg caccaccgta attttcgtac ccttgttgat ctcggtaatc ctgcgggctg
     1861 taatcgaggg ctcttcacgc acggtcgtag cgcggatgac ctcgtaaact cccggctcag
     1921 cagaacgacg ggccgctggc gtctgtgaag gcggcgtttt aacggaacgc gccgatgctc
     1981 ctggcgctat tgccgggcgc tttgctgcta ccgctgccgc ttcgcgcgtc gcgccggtta
     2041 atcgttgctg agtcgcggac agttccttct ggttttcttc aagctttgcc gcaagtgacg
     2101 tcagctcgtt tttgctttta tccagttctt gccggagctg tctcagttct ccttcaagtg
     2161 ccttaatttt ttcctcgcgt tcttgcacct gcttagagtt ggcctcttcc agtcgccgag
     2221 tatcgtttct ttgcccatac ccagcgatca agtaaaggcc gaaaccaaga acgattatca
     2281 agcttgcgcc cagtgtcgcc atcgcgcgct ttgtccaagc ccgtctgcca ggctggccgc
     2341 gggtacgagc cggttggcca gccatctcat cgcgactggg ccattgcggt ctctcctccg
     2401 tgctgccgaa ctccgccttg caataaggac atctcacagc atcttcctca atgctgttgg
     2461 cgcagtgcgg gcaaagccgg gcgcggttcg tggtcgcgat caccagcgct ctgccatcct
     2521 tggtacagaa actggtgtta tcgtcatact cagtggaaca attgggacag cgtttcatgc
     2581 cagacattgt tagatgaata agcttacggg tcaagggaaa gcatgctcgt gccgcttgcc
     2641 ataagccaca agcatcggat tttcttcttt gtaccgtcac aaccaatagc cactgtttgt
     2701 tgccgggatt ttgccgttgc gctatagaac gttggactaa ctctgggtac gctgcgcaaa
     2761 agatctcata ggcaaagcgc gcgaatttcg acgagctgag gcgtaggcta cggtacgttg
     2821 aagcctctct cccaagcgcc acgaagcagc tccgatagaa cgcatggagg atgacaacga
     2881 tatggcttta ctcattggag aaccgcaaac cagaaagctt atcaacatgg cgcgtgcggt
     2941 cgatcttctc gacaaaatgt ttctagatcg ggccgccggc aagatacgcg gtttgccacg
     3001 ccggcggctc aagggaaacg agaagcaaat caacatcatg gctgcctggc atcagggtta
     3061 cgacctgttt tgcttgcgcg cttacatcgg tcaagcaaat acgatcacgc tctataacgg
     3121 caagagcggt cagatccagg caatcatcaa tatgggctat ctcagtagcc tccggaccgg
     3181 cgctgcgacc ggtgttgccg caaagtatct agccccgcga aatgccagta ctctgggcgt
     3241 tattgggccg ggctggcaag ccacctttca ggtggaagca gttgcacacg cgtgtcggat
     3301 cgaacggatc gtggtgttcg gaaggactcc caagcggcgc aaacaattca tcaaacagat
     3361 gagccagatc gttaaagccg tgtggcacga ggcatcctct gttgaagagg ttgaacgcga
     3421 gtcggatatc cttgtcgttt cgaccaactc caccactcca gttgccgacg gaaagaacct
     3481 taaaacggag gcgctggttg cttccatcgg cgcaaacgca gccatcaaac acgaagtctc
     3541 aggcaacttg atcgggcaaa tggatctcat catcgtcgac gacgtggccg ccgcaagaac
     3601 agacagtggt gatttgatcg atgcttgtca gaacggcatc gcgcgttggg aggatgttct
     3661 tccgctagaa aaatttgccg gcagcggccc ctcgcaaccc cggcccaaga gaatcttttt
     3721 tcaatccaac ggtattgctg atgaagatct cgccgtgggg cgatatgtgc tggagcaggc
     3781 gaagcgaaaa ctcaagcttc gccaggtcac ggagatatag cttagttggg gcgctgccga
     3841 cagacagccg tgtcgcatca gattcgaaag cagtcgacac cttttgaatc aggttcttgc
     3901 cgccgcctcg gattagatac ccggctcagc tagcttacat catccccgag tcttgtattt
     3961 tgctctaggg tttctggaag gccagtacga agcggtcgct cagatttcgc tgtttgttta
     4021 cgttgaaatc ccgtttatct tctggccggc taagtatcgt cccttctttt gccagtttga
     4081 accctgaacc ggtcacttct ttgatgacca atagtttatc gatgcgatga agcgttttca
     4141 ctgcctcgtt gccgctcccc tctttggctg agtgatcgac cacaccataa acaccgcctg
     4201 gcttcagagc gccatttatt ctcttgttca tcgcgatacg gacatcggga gagagatcgt
     4261 gataattcaa atgtatcaag gcgaaatcga gcgagccgtc ctgcggcaag gccagtttgt
     4321 ccaatggtcc ttcgactatc cggacgttgg cgaacttcgg atttctgaag cgttcagtca
     4381 gctccggccg gggtgccgga actacagagt agaccattcc acggggtccg accacttgag
     4441 atagaatagc cgtgtaatag ccgcgccccg cgaatacatc ggcgacttta tcgccgcttt
     4501 taactccata aaaagcgagc acctcgttcg gtttgcgggt ggagtccagc gcccgctcag
     4561 tatccggtcg ttctttatcg gcgaggatcg atgaaaaatc ctgctgcgac tgcgtcgtcg
     4621 cgcagccggc aagagtgagg attgatagta gaccgataac gaaaactcgt tgcgagagca
     4681 cgttgagcat aactcctcc
//
LOCUS       DASUTG010000331         5912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1237945, whole genome shotgun sequence.
ACCESSION   DASUTG010000331 DASUTG010000000
VERSION     DASUTG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237945"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF089_07565"
     CDS             <1..708
                     /locus_tag="VF089_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6173858.1"
                     /translation="GTTVTAAPGYPALATKHLSDNTGIEFCLAEDLIEATSDTGDYVL
                     LSGVIKHTATKLTKICNDLRMLASGPRCGLNEINLPQMQPGSSIMPGKVNPVIPEVVN
                     QTSFLVIGLDVTVTLAASAGQLQLNVMEPVITFALFASIATMERAVNSLRVNCVQGIT
                     ANDERTRDMVLNSLGIVTVLKPSLGYKQCAELAREGYETGKSLHDIVVNEKKLFSQQK
                     WDEIFSFENLIKPKFEQ"
     gene            1074..2144
                     /gene="cax"
                     /locus_tag="VF089_07570"
     CDS             1074..2144
                     /gene="cax"
                     /locus_tag="VF089_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX6173859.1"
                     /translation="MNWLLIFVPIAVALEHVAPQAHTWIFFSACVAIIPIARLIVLAT
                     EQISTRTGEAVGGLLNATFGNAPELIITLVALKAGYADMVRGSLIGAILANLLLALGL
                     GFLFGGFRHVDQKFNSIAARAYSTMMLLAVTSLIVPSSFSRFFAPEGTIRQEQLLNIG
                     IAFVLLAVYSLYLVFSLKTHPHAFASLEQEGTHHEGPEWSLWQAIGGLLGAAILAAWM
                     SEILVGAAEGTGKALGMSQSFVGIVFLAIVGGAAESSSVIVTARKNRMDLSVSIALGS
                     CIQIALFVAPVLVLASYFIAPQPFELSFSRAEVGSLFLGVLIGTLVCGDGQSNWFKGV
                     QLITVYVIMALLFYFMPEVMPG"
     gene            2141..2824
                     /locus_tag="VF089_07575"
     CDS             2141..2824
                     /locus_tag="VF089_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX6173860.1"
                     /translation="MSGFSQVRAGSPGALGNAYLCRRYEILFYTLLLTLMAAPLAAAL
                     ELKGALLELLLAANLLAAVMPISTGRGRRVLLAILAVLWLARAATAWFGYSAFSAVTL
                     GLWVLLAMFAAGAALRFAMRASRVDAEHLYAALSAYLLAGIFFGIFYWVLERLGPGTF
                     AAPAEFSRMSAIYFSFVTLATLGYGDIVPRTDIARGLAIVEGVGGQLFLAVMVARLVS
                     LYAREKAAE"
     gene            2898..4049
                     /locus_tag="VF089_07580"
     CDS             2898..4049
                     /locus_tag="VF089_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173861.1"
                     /translation="MNLRKILSWLLFIVPVAGCDLASVRQPAGSTPYASPHIGVASGA
                     QSSSPRSVLQPQGSTGSQRTQYAWEKALEGMAIGGAIGGIYGAGGGLLIGFLTGFFTA
                     EIYDDQLNTQMQSEQTKDKLLEAKIEQELERQRQLETQLLNSPASPQQDHAGLPQSAL
                     SPLESTASRVAMNDGSSTLASLSRRESPSSPTSSPFKNVEVRDINGDGVPDLWIYYSP
                     FSSSEIVRQEEASQFDGRVDSWSHFKDGQLVRREVDTKGKGLADTVYYYENEKIVREE
                     RDDNGTGGVSFRTLYQNGRRVKIEEDTSGRGKIDRWIYFDSDRDGEIVLKEERDLNGD
                     GVVDLWSYYEDGRLVRRDVNAVGVELLSEQDRFPFPAPDTKNLSQSPAG"
     gene            4124..5500
                     /gene="zwf"
                     /locus_tag="VF089_07585"
     CDS             4124..5500
                     /gene="zwf"
                     /locus_tag="VF089_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6173862.1"
                     /translation="MKQETADAVVFFGATGDLAYKKIFPALQALAKRGNLSVPVIGVA
                     KAGWTLDQLKSRARDSVENYGGLDPAAFAQLSRCLRYIDGDYAAPATFQLLRRELGDA
                     QRPLHYLAIPPSLFEIVIHQLGASGCAKGARVILEKPFGHDLGTAQMLNSVVHSSFNE
                     ENVFRIDHYLGKNTVQNLLFFRFANSFLEPLWNRQFVESVQITMAEDFGVDGRGKFYD
                     QTGAVRDVVQNHLLQVLSNIAMEPPPGLDAEMLRDEKAKVLKGIRPLEPRDVIRGQFR
                     GYRDEPGVSANSQVETFVALRLEINSWRWKGVPFYIRAGKCLPVTATEVLVKLRQPPA
                     VFSEVAPPPNYFRFRVTPDLMIAVGALVKKAGQREGQNVELVISEESDPAEMGAYEEL
                     LFDAMRGNSARFARQDYVEEAWRIVDPVLGDVVPVYEYEPGTWGPSEVSAFNGPDGGW
                     FAPSSRQS"
     gene            complement(5598..>5912)
                     /locus_tag="VF089_07590"
     CDS             complement(5598..>5912)
                     /locus_tag="VF089_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6173863.1"
                     /translation="DQLSDAILIIEMGTIDIVVKGKQAPVATMGNGQTLGELAFFEPD
                     KRFATATARERTELVRIPFDKLAGLLTDRPALALLFYRNACAFFAKHIRELGSERDRR
                     YF"
BASE COUNT         1264 a         1591 c         1736 g         1321 t
ORIGIN      
        1 ggcactaccg tgaccgcagc acccggttat cccgcgctcg cgaccaaaca tctttccgat
       61 aataccggca tcgagttctg tttggccgag gacctgatcg aagccacttc cgacacgggc
      121 gattacgttc tgctatccgg cgtgatcaag cacaccgcga ccaagctcac caagatctgc
      181 aatgacctac gtatgctggc ttcagggccg cgctgcgggc ttaacgagat caacctgccg
      241 cagatgcagc cgggctcctc gatcatgccg ggcaaggtga atccggtgat tcctgaggtg
      301 gtaaatcaga ccagcttcct ggtgatcggc ctcgacgtca ccgtgacgct cgccgcttcg
      361 gccgggcaac tgcagctcaa cgtcatggag ccggtgatca ccttcgcgct ctttgcgtcg
      421 attgcgacga tggagcgcgc cgtcaacagc ctgcgggtga attgtgtgca aggcatcacg
      481 gccaatgacg agcgtacccg cgacatggtg ctcaattcgc tcggaatcgt gaccgtgctc
      541 aagccttcgc tcggttacaa gcagtgcgcc gagctcgctc gcgaaggata cgagacgggc
      601 aaaagcctcc atgacatcgt cgtgaacgaa aagaagcttt tcagccaaca gaaatgggat
      661 gagatcttct cgttcgagaa tctgatcaag cccaagttcg agcaatgagc ccgaagctgc
      721 aaactcttcg ctgaagataa gtcaattgcc actttgcccc acctcatggg cgtccttcca
      781 gcgatctcga gggaaacgcg gcatggcggc gtctgagcgg gggaactgct tgtcacttca
      841 gcatggagcc attggagtgt tcccttcggt caacgcctcc tcctgagcta gggctcgctg
      901 atagccgcga ttagctttga cctgtccgac accgtctaga tgggtaagta cttgcttaac
      961 caatctcggg cgtatggccg gacgaggcgc tcgccatcga ccgtgacttt gtcaaaagag
     1021 taacgggcac tgattaattt aatcaacgcc atagacagga gcttgtggtg acgatgaatt
     1081 ggcttctcat ctttgtaccg atcgccgtgg cgctcgagca cgtcgcgccg caggcgcaca
     1141 cgtggatttt ttttagcgct tgtgtcgcca taatccccat cgccaggctc atcgtgttgg
     1201 ctacagaaca aatctccaca cgcacaggcg aagctgttgg tggcctactc aatgcaacct
     1261 tcggcaatgc gccggagctg attatcaccc tcgtggccct gaaggcgggt tacgcggaca
     1321 tggtgcgggg atcgttgatc ggggcgatcc tggccaatct gctgctcgcc ttaggcctcg
     1381 gatttctgtt cggaggcttt cgccacgtcg accagaaatt caattcgatc gccgcacgag
     1441 cgtacagcac gatgatgctg ctggcggtga ctagtctgat cgtgccgagc tcgttcagca
     1501 ggttttttgc gcctgaaggc acgattcgac aggagcaatt gctgaacatc ggcatcgcct
     1561 tcgtgctgct ggcggtctat agcttgtacc tggtgttttc gctcaaaaca catccacacg
     1621 ccttcgccag tttggagcag gaagggactc atcacgaagg tccagagtgg agcctgtggc
     1681 aagccatcgg aggcctcttg ggtgcggcca tactggcggc gtggatgagc gaaattcttg
     1741 tcggagcagc ggagggcact ggcaaagcct tgggtatgtc acagagcttc gtcgggattg
     1801 tgtttctggc aatcgtcggc ggggcggcgg agagcagctc cgttatcgtg acggcgcgca
     1861 agaacaggat ggatttgtcg gtgagcatcg cattgggcag ttgcatccag atcgcactct
     1921 ttgtcgcacc ggtactggtc ttggcgagtt acttcatcgc accacagccg ttcgaactct
     1981 catttagccg cgcggaagtc ggatcgctgt tcctcggcgt attgatcggt accctggttt
     2041 gcggcgacgg tcagtccaac tggttcaagg gtgtgcagct catcacggtg tacgtcataa
     2101 tggcgttgtt attttacttt atgcctgagg tgatgccggg atgagcggat tcagccaggt
     2161 tcgagccggc agtccaggcg cgctcgggaa tgcctatctc tgccgccgtt acgagatctt
     2221 gttctacacc ctcctcctta cactgatggc ggccccactt gccgctgctt tagagttgaa
     2281 gggagcgctg ctcgagttgc tgctagccgc caatctgctt gcggcggtaa tgccgatcag
     2341 caccggcaga ggtcgccgcg ttctgttggc aattttagcc gtgctatggc tggcgcgtgc
     2401 ggcgaccgca tggtttggtt attcggcatt ctcggctgtg acacttggtc tgtgggtgct
     2461 gctcgccatg ttcgcagcgg gcgccgcttt gcgatttgct atgcgagcct caagggtaga
     2521 tgcggagcat ctgtatgcgg cgcttagcgc ttatctgctc gcgggaattt tcttcggcat
     2581 cttctattgg gttctcgagc gcctcggacc gggtacattc gccgcacccg ctgagttctc
     2641 gcgcatgagc gcgatctatt ttagtttcgt tacactggcg acgcttggct acggcgatat
     2701 cgtccctcgc acggacattg ctcgaggtct tgccatcgtt gaaggtgtcg gcgggcagct
     2761 ttttttggcg gtgatggttg cccgcttagt cagcctctat gcgcgggaga aagcagccga
     2821 gtagggacct gacacggtga gaaaatgttt agctaagcgc tgagaaagag tcgccgccag
     2881 agcgacgagg aggaaagatg aacttaagaa aaatcctctc ttggttgctt tttatcgttc
     2941 ctgtggccgg gtgcgatctt gcatcggtcc ggcagcctgc tggctcaact ccttatgctt
     3001 ctcctcatat aggcgttgcc tcaggcgccc aatcctccag ccctcgatca gtcctacagc
     3061 cccaaggttc gactggatca cagcgaactc aatacgcctg ggaaaaggca ttggaaggca
     3121 tggctatagg tggtgctatc ggtgggatat atggagccgg agggggtttg ctcatcggct
     3181 ttctcaccgg gttcttcact gccgagatat atgacgacca gctgaatact cagatgcaat
     3241 ccgaacaaac caaagataaa ctgctcgaag cgaagatcga acaggaattg gagcgtcagc
     3301 gacagctgga aactcaactg ctaaatagcc cagcgagtcc gcagcaagac catgcaggcc
     3361 tgccgcagtc ggcgctaagc ccgcttgagt caacggcatc cagggtcgcc atgaacgacg
     3421 gttcaagtac actagcttcg ttgagcagaa gagaatcccc ttcaagcccg acgagcagcc
     3481 cgtttaagaa tgtcgaggtg agggacatca acggcgacgg cgttccggac ctatggattt
     3541 actacagccc atttagctcc agcgagatcg tgcggcaaga ggaggccagc cagttcgatg
     3601 gcagagttga tagctggagt catttcaaag acggacagtt agtgagacgt gaagtcgata
     3661 ccaaaggcaa gggcttagcg gacacggttt actattacga gaacgaaaag attgtcaggg
     3721 aagagcgtga tgacaacggc accggcggtg tgagtttccg cacgctctat caaaatggcc
     3781 gtcgcgtcaa gatagaggaa gataccagtg gccgcggcaa aatagatcgt tggatctatt
     3841 ttgactcaga cagagacggc gagattgtct taaaggaaga aagggacctc aatggcgacg
     3901 gagtggttga tctgtggtcc tattatgaag atggtcggct cgtccgacgc gatgtaaacg
     3961 cagtcggagt ggaactcctg tccgaacaag atcgattccc ttttcccgcg cccgatacaa
     4021 aaaacctttc gcagtcccca gccggttaga gacgactaag gacgaggcga aatccgttac
     4081 cttcactcgc acgcggccaa cccgggtgta aaacgaggag ataatgaaac aagaaaccgc
     4141 cgatgctgtg gtttttttcg gggcgactgg agatctcgcc tataaaaaaa tattccccgc
     4201 cctccaggcg ctggccaagc gcggcaatct cagcgtcccc gtcatcggtg tcgccaaagc
     4261 tggatggacg ctggatcaac tgaagtcgcg cgcccgcgac agcgtcgaga attatggcgg
     4321 gcttgatccg gctgcttttg cccaactaag ccgttgcttg cgctacattg atggcgacta
     4381 tgccgctccg gcaacctttc aactgctccg gcgggaattg ggcgacgcac agcgtccgtt
     4441 gcactatctt gccattccac cgagcctttt cgagatcgtc attcaccaac tcggcgcgtc
     4501 gggttgtgct aagggcgcgc gggtcattct cgagaagcct ttcggacacg acttgggcac
     4561 ggctcaaatg ctgaactcgg tggtacacag tagtttcaat gaagagaacg tgtttcgcat
     4621 cgatcattac ctgggcaaga acacggttca aaacttgctc ttcttccgct ttgcaaattc
     4681 atttcttgag ccgttgtgga atcggcagtt cgttgaaagc gtgcagatca cgatggcgga
     4741 agatttcggc gtcgacggtc gtggcaagtt ttacgatcaa acgggcgccg tgcgcgatgt
     4801 cgtgcagaac catctgctgc aagttctttc gaacatcgcc atggagcctc cgccgggcct
     4861 cgatgccgag atgttgcggg atgagaaggc aaaagtgctg aaagggattc gtccgcttga
     4921 gccgcgcgat gttatacgcg gtcagtttcg cggctaccgt gatgagccgg gggtcagcgc
     4981 aaattcgcag gtggagacct ttgttgcgct cagactcgag atcaactcgt ggcggtggaa
     5041 gggagtgccg ttttatatcc gggcgggcaa gtgcctcccg gtgacggcaa cagaagtact
     5101 cgtcaagttg cgccagccgc cggcggtttt ctcggaggtc gcgccgccgc ccaactactt
     5161 ccgtttccgc gtaacacccg atctgatgat tgcggtcggc gcattggtaa agaaggcggg
     5221 tcaacgcgaa gggcaaaacg tcgagctggt catcagcgag gaatccgatc cggccgagat
     5281 gggagcttac gaggagcttt tgttcgacgc gatgcgggga aattcggccc gttttgcacg
     5341 gcaggattac gtcgaagagg catggcgaat cgtcgatcca gttctgggcg atgttgttcc
     5401 tgtttacgag tacgaacccg gcacgtgggg gccatcagag gtgagtgcgt tcaacggacc
     5461 cgacggcggt tggtttgccc cgagctccag acaatcctga tgggatttac ccgctgaaaa
     5521 gagccagcac atcatgacgt gagcataacc acaatcaagt cggatcattt tcatagatga
     5581 tgggcgcaaa caagtgtcta gaagtaacgg cgatcacgct ccgagcccag ctctcgaata
     5641 tgcttggcga aaaacgcgca cgcgtttcgg taaaaaagaa gggcaagtgc tggccgatct
     5701 gtaagcaacc ccgctaactt atcaaaaggt atccgcacaa gctccgtgcg ctcgcgggcg
     5761 gtagcggtag cgaaccgttt gtccggctcg aaaaaagcga gttcacccag cgtttggccg
     5821 ttccccatcg tggcgaccgg cgcttgtttt cctttaacga cgatgtcgat ggtgcccatc
     5881 tcaatgatca aaatcgcgtc ggaaagttga tc
//
LOCUS       DASUTG010000332         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1683396, whole genome shotgun sequence.
ACCESSION   DASUTG010000332 DASUTG010000000
VERSION     DASUTG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683396"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1070)
                     /locus_tag="VF089_07595"
     CDS             complement(255..1070)
                     /locus_tag="VF089_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6173864.1"
                     /translation="MAELRTILQKVDEDAGVAWLTFNRPEKKNALSQRLMIEMVEILK
                     ELSDNDKIKCIVTTGSGDSYSSGLDLYDLRENWKRKRPWDQAGSTYEIVRLLRNMPQV
                     TVAAVRGWCLGGGLALTNGHDLCIAAESAKFGMPEVIRGSYGAVATSTLFHSGIPFKK
                     AFYIQLTGRNLTAEQAERVGLVSEVVPDDQLTAAVEQLAKELGSRNAVTLESAKIAAY
                     MQKDMDFLTALRTDDLVSHRMRYYTNPLNDVEGYLHSQKGGGTTKYVKPEDRK"
     gene            complement(1156..2022)
                     /locus_tag="VF089_07600"
     CDS             complement(1156..2022)
                     /locus_tag="VF089_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6173865.1"
                     /translation="MNLELRDHYFTYDGLKLHYWEWGDPKEETFVFVHGVRDQGRSWD
                     DFIAAMIARGLPIKHAVAIDLRGHGDSEWPSTSRGYQHEDFLTDLAGLLRHLDKEPLT
                     IIGHSLGGSMCLLYAGTFPEKVERMVLLESLGPFARADEEVPEIFAERLNGRDYVEIP
                     FPHESLEAAAKALQKTYPLIPDNAALHMARYGTSFKGGRYRWKYDPILRYRTTTALSE
                     GQIEAFIRRVKCPIFFVYGTESDFMKSVRGPRVKLFPNAEIVPIEGAGHHIPHERPQE
                     LAEIVVPFLLRD"
BASE COUNT          495 a          636 c          541 g          552 t
ORIGIN      
        1 gaccaactcc ggcggcgttg tcataccttc tttgtcccag cccatgccgg cctgaatgat
       61 gggaacttcg atacctaaca gatcacagag tggtgttctc agcttcatat ttccttccta
      121 aggcttaaga tcgtgcttgg aaaagtaccg tgtcgtgttc ggaataagca ttacccaacc
      181 agatcacgcc gtcgcgggta cggctttccg gcacacaaac acgatcacgg cactttccga
      241 tcacgaacgg ctcgttattt ccgatcttcc ggcttgacgt atttagtagt accgccgccc
      301 ttttgtgaat ggagatagcc ttcaacatca ttcaatggat tggtgtagta tcgcatgcgg
      361 tgcgaaacca gatcgtctgt tcttagcgcc gtgaggaagt ccatgtcttt ttgcatgtaa
      421 gccgcgatct ttgcgctctc cagcgttact gcgttgcgac taccgagctc tttggccaat
      481 tgttcaaccg cagcggttag ctgatcgtcc ggcacaactt ccgaaaccaa gccgacccgc
      541 tcggcctgtt ccgcggtaag atttctgccg gtcagttgga tgtaaaaagc tttcttgaat
      601 ggaatgccgc tatgaaacaa tgtggaggtg gcaactgcgc cataactgcc acggatcact
      661 tcgggcatgc cgaacttggc cgactcggcg gcaatgcaca gatcgtgacc attggtcaat
      721 gccagacctc cgcccaggca ccagccgcgc accgccgcaa ctgtgacctg cggcatgttg
      781 cgcagcagcc ggacgatctc atatgtcgaa ccggcttggt cccaaggccg tttgcgtttc
      841 cagttttcgc gcaaatcgta aagatccagg cccgatgaat acgagtcccc cgaaccggtg
      901 gttacgatgc atttgatttt gtcattgtcg ctgagctctt ttaagatctc gaccatctcg
      961 atcatcagcc gctggctcaa agcgtttttc ttctccggtc gattgaaggt cagccaggct
     1021 actccggcgt cctcgtctac tttttgcaga atcgttctta attcagccat catttacctc
     1081 cagtgataca ttcaaacgtg cgtcgagaac cttggttatc cgagttccct ttaggctaac
     1141 gcctaaaagc gtagcttaat cccgaagcag aaagggcacg acgatctcag ccaactcttg
     1201 gggcctttca tgcggaatgt gatggccggc tccttcgata ggcacaatct cagcattagg
     1261 aaagagctta acccgcggac cgcgcaccga cttcatgaaa tcactctctg tcccatagac
     1321 aaagaagatc ggacacttga cccgccggat aaacgcttcg atttgtcctt cggataaagc
     1381 ggtagtcgtg cgatagcgga gaatcggatc gtacttccaa cggtatctac cgcccttgaa
     1441 actggttccg taccgcgcca tatgcaacgc ggcattatcc gggatgagcg gataggtttt
     1501 ttgcagcgcc tttgcggccg cttccaaaga ctcgtgtgga aacgggatct caacatagtc
     1561 tcgcccgttg agtctctcgg caaagatctc aggcacttct tcatcagccc gcgcaaatgg
     1621 accgagcgat tcgagaagca ccatccgctc gactttttca gggaaggtcc cggcgtacag
     1681 caggcacata ctgccgccca gtgaatgccc gatgatcgtc agcggctcct tatccagatg
     1741 gcgcaggagg ccggcgagat ccgtaaggaa atcctcatgc tgatagcctc ggcttgtgct
     1801 tggccattcg ctgtcgccat ggccacgcag gtcgatggcg actgcgtgct tgatcggcaa
     1861 gccgcgcgcg atcatcgccg caataaaatc atcccagctt cgcccctgat cgcgcacgcc
     1921 gtgaacaaaa acaaaagtct cttccttggg atcaccccac tcccaatagt gaagtttcaa
     1981 cccatcataa gtgaagtaat gatcccggag ttctaaattc atcctggttt tttgccgcct
     2041 ttcaaataca gttatgcaat cagatcgagt tttagtgact ccatatgccc atttattctc
     2101 ttactacctc ctgcctaatt ctttaagcgc ttcatcgagg taactgtggt cgacgtactt
     2161 ggtcggactc ggcaatggcg gctttaactg tccccgctcg gccatgatct gaataacagc
     2221 ctgg
//
LOCUS       DASUTG010000333         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1090232, whole genome shotgun sequence.
ACCESSION   DASUTG010000333 DASUTG010000000
VERSION     DASUTG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090232"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /locus_tag="VF089_07605"
     CDS             complement(<1..1533)
                     /locus_tag="VF089_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173866.1"
                     /translation="MLAIIRRISVGLAFLLLILIIGVAAITHTDRFREFVREQMVVAI
                     NDSIAGSISLGRLEGSIWGNAILHDVVVSHEGTEILQLPRLTISYSLFPLIWGRLHIF
                     QLTGSRPSLHLRRAEDNELNIVQAFSPRQPQSEGDSGLVVLLNSIILREGHIDLSLAG
                     PKPQNYSLDDLSLEGRIHVRPKNVTIVLSRVASRFTTPGFPELSVNGAMVYHETAGTD
                     TFEVANLLVSTGESHARVSGKLTDLQAVNVDAKIAVEKLAPSDVRGLFADWPLRRDVA
                     GAITINGPLDALVVDVNLAAAGARLAASLQIETAQETPRYRGTINLTGFDVRTLLGRN
                     DVAGVLNANIEGQASGLELSAVDAKGVIKMQSAVVGGWSLGDISIDGRLRENVATVAG
                     RLKSKIGGASWNGEISFKDKPAYDLALSVDNLDIQKTVSGADGLEGNLNFKGTLKGAG
                     LTPSDIVASTELKILPSMIGPVEVQQGSLVANLAQQRLQIVKATARTQDAMLTVQGDV
                     GLD"
     gene            complement(1545..3377)
                     /gene="bamA"
                     /locus_tag="VF089_07610"
     CDS             complement(1545..3377)
                     /gene="bamA"
                     /locus_tag="VF089_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX6173867.1"
                     /translation="MKFTASLISAMAMWLLLACRASAVQVEALDPSREWRVDKIEISG
                     NDHFSDSQVMAEMITQSRPWYRFWEERPLFDPVTFATDLARLRRFYEAEGYYRSGITH
                     DLVVDETRSLVSPQVFVEENRPVVISQIKVEIETEPTEQDHPRLPEKLPVTRGEIFKE
                     QEYQDGELLLRNYLMDNGHAHAQTQRRAEIDLEQDQVRIRYSAQPGPKAVFGETEIKG
                     TEQVEPYLIVRELAYEPGEMFSLKKIRESRQQILALDLFSALRINPRESAGTSTVVPM
                     EVQVSEKPPRELSFGIGYSTEEEFRVSLDWRHQNWLGDGRRLSIRGKYSSINASGAID
                     FIQPHFLTRRTQGGLNLRHEYQEEETFHRTATRFSGRLEHRFSPRLLGFADYRIENNQ
                     LTEVSAATPQALGNIRTKGILTGPILGLVWNSTDDLFQPTRGDIVSISADQGGGIWGG
                     QYEFFKLRGEAKKYLAIGWETILAGRLKLGVADAIGSKKNYPLFERFFAGGQSSVRGF
                     ERHRLGPLNSDDEPLGGLSLIEGSVELRRPVWRELGGAVFVDFGQVSLKTFDIPVNDL
                     QFSTGFGLSYNTPVGPLRLDIGFPFNPPRGDRAWQIHFSVGAAF"
     gene            complement(3612..3983)
                     /locus_tag="VF089_07615"
     CDS             complement(3612..3983)
                     /locus_tag="VF089_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX6173868.1"
                     /translation="MKAIGARLWAIPEGYIPGWSNGPEPAFTSHETVCLLNTADQDAH
                     VLITIFFSDREPVGPYSVTVGARRTKHVRFNDLSEPEKIPTATDFASVIESDVPIVVQ
                     HTRLDSRQSENALLSTIAYRE"
     gene            complement(4072..4300)
                     /locus_tag="VF089_07620"
                     /pseudo
     CDS             complement(4072..4300)
                     /locus_tag="VF089_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4606..6216
                     /locus_tag="VF089_07625"
     CDS             4606..6216
                     /locus_tag="VF089_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6173869.1"
                     /translation="MSANKPAIQPRTCPPPLDESILVSLYQGIPEAIVVIEFKSRCIV
                     YWNEGATLLFGYSAQEVLQQTTERLYLDKNWFDRIYQTSIPDIEQNGCWRGEWQYRRR
                     DGSLFAAEATCTLLQTDKGAYVVKVIREVTERKEAEKIILELNQRLTQRVIEQTSELM
                     SKTEELQHEEAQRKQVEQILGNLLRNITNYAVLITDADGRIVDWNRTATRILGRESDD
                     MADQRLSVFFDEEEGRQALIKKALIEASDQGTFRDYQRLVRKDGSRFYARVIVFALRD
                     DADGLQGYLILLRDDSQERNLREKLREKEHLAAIGTAASMLAHEIGNPLNGISTTVQL
                     LERFMIREGIKVPPHMLATLKDLKSEIDRLTSLLNEFKTIARPQKLSIQPVHLASVIR
                     ETISGLEMEGKGDVQIVSDCSPDLPPVRGDAAKLKQAFLNLFKNAVEAMPYGGTLSIK
                     GYWSGGSICVDISDTGIGIPDDLKVFDLFSSTKPHGTGLGLFIVQQIVLAHDGVVSYA
                     STVGKGTTFHLTLPASAAPEAVADIRRN"
     gene            complement(6268..>6518)
                     /locus_tag="VF089_07630"
     CDS             complement(6268..>6518)
                     /locus_tag="VF089_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX6173870.1"
                     /translation="RGQTLLRQIDDALERIDAGTFGECERCGNDIGHGRLKAMPTAAL
                     CIDCATALEKRQRMLGAHGDVERLPFKDDELDRSLGSE"
BASE COUNT         1463 a         1786 c         1762 g         1507 t
ORIGIN      
        1 atcgagacca acatcgcctt gcaccgtaag cattgcatcc tgagtcctgg ccgttgcctt
       61 gacaatctga agtcgctgct gtgcgaggtt ggcaaccaaa gaaccttgtt gcacttccac
      121 cggcccgatc atcgatggca agatctttag ctccgtgctg gcgactatgt cgctgggggt
      181 gagtccggca cctttgaggg tgcctttaaa attgaggttt ccttcgaggc catctgcacc
      241 gctcacggtt ttttggatat caaggttatc taccgatagc gccagatcat acgccggttt
      301 atctttaaaa gagatctcgc cgttccatga ggcgccgccg atcttgctct taagtcttcc
      361 agcgacggtg gccacgtttt cgcgcaggcg gccgtcgatc gaaatatctc ccaagctcca
      421 tcctccaacc accgcagatt gcatcttgat cacgcctttg gcatcgacag cggaaagctc
      481 aagaccactg gcttggcctt cgatgttggc gttcagaacc cccgccacat cgttcctgcc
      541 gagtagcgtc cttacatcaa agccggtcaa gtttatcgtt ccacgatagc gcggcgtctc
      601 ctgcgcggtt tcgatctgga gactcgccgc caacctggcg cccgccgcgg ctagattgac
      661 atcgacgacc agcgcatcga gcggcccatt gatggtgatg gccccagcaa catctcgtct
      721 aagtggccag tcggcaaata aacctctgac atcagacgga gcaagctttt ccaccgcgat
      781 tttcgcgtcc acgttaacgg cctggaggtc agtgagtttg ccgctcactc tcgcgtgcga
      841 ctctcccgta cttaccagaa gattcgctac ttcaaacgtg tcggtgccag cggtctcatg
      901 gtagaccatc gctccattta cgctgagttc cgggaagccc ggcgtagtaa agcgcgatgc
      961 aacgcggctg aggacgatgg tgacgttttt gggtcgaaca tggatccggc cttcgagact
     1021 caggtcatcg agagaatagt tttgtggttt tggacccgca aggctcagat cgatatgtcc
     1081 ctctcgcaag ataattgaat tcaagagcac cacgagcccg gaatctccct cgctctgtgg
     1141 ctgtcttggg gaaaaagctt gcacgatgtt cagctcatta tcttccgccc gtcgcaaatg
     1201 aagtgacggc cggctgcccg tcaattggaa aatgtgcaag cggccccaaa tcagtggaaa
     1261 cagagaatac gaaattgtta gccgcggcaa ttggagaatt tcagtgcctt catgacttac
     1321 aacgacatcg tgcaaaatag cattgcccca tattgatcct tccaatcgtc ccaggctgat
     1381 ggagccggcg atcgagtcgt tgattgctac caccatctgt tcgcggacga attcacgaaa
     1441 acgatcagtg tgagtaatgg ctgccacccc aatgatgagg ataagcagca aaaacgcgag
     1501 cccaacactg atccgacgga tgatggccag cacccttctc agggttagaa ggcagcccct
     1561 acgctaaagt gaatttgcca agcgcgatcg ccgcgtggcg ggttgaaagg aaaacctata
     1621 tcaagccgca acggtcccac gggcgtgttg tagctcaggc cgaagccggt tgagaactgt
     1681 agatcattga cgggaatgtc aaaggttttc aaagacacct gcccaaagtc aacaaatacg
     1741 gccccgccta gctctcgcca gactggacga cgtagttcca ccgaaccttc aatcaggctg
     1801 agaccgccga gcggttcgtc gtcactgttg agcgggccga gccggtgtct ttcaaacccg
     1861 cggacactgc tttggccacc ggcgaaaaag cgttcgaaca aaggatagtt tttctttgaa
     1921 ccgatagcat cggcaactcc cagctttaaa cgtccggcca ggatcgtttc ccacccgatc
     1981 gcaagatact tttttgcctc gcctctcaac ttgaagaatt catattggcc accccagatg
     2041 ccgccccctt gatccgcact tatggacact atatcgcctc gggtcggctg gaacaggtca
     2101 tccgtgctgt tccaaaccaa gcccaagatg ggacccgtca aaatgccttt ggttctgatg
     2161 ttaccgagtg cctgaggcgt cgctgctgac acctcggtaa gttgattatt ctcgatgcga
     2221 tagtccgcaa aaccgagaag cctcggcgag aagcgatgct caagccgtcc ggaaaaacga
     2281 gtcgcggtgc gatggaaggt ttcttcttct tgatattcgt ggcgcaggtt gagcccgcct
     2341 tgggtgcgcc gtgtcagaaa gtgaggctga atgaaatcga tcgcgccgct ggcgttgatc
     2401 gaagagtatt tgcctcggat cgataaacgc cttccgtctc cgagccaatt ttggtggcgc
     2461 caatccagag agactcggaa ttcttcctcg gtgctgtagc cgattccgaa actgagttcg
     2521 cggggcggtt tttcactaac ctgaacttcc atgggaacca ctgtcgaggt tccggcactc
     2581 tcccggggat tgattctaag cgcgctgaat agatccaacg ccaggatttg ctggcgggat
     2641 tcccggatct tttttagcga gaacatttcg ccaggctcgt aggccagctc tctcacgatt
     2701 aaatagggct caacctgctc ggttcccttg atctcggttt caccaaacac cgccttgggt
     2761 ccgggctgcg ccgagtagcg gatacgaacc tgatcttgct cgaggtcaat ctcggctcgg
     2821 cgttgtgttt gggcgtgtgc gtggccgttg tccattagat aattgcgtag caacagttcc
     2881 ccatcctgat attcttgttc cttgaaaatt tcgccgcgcg tgacgggaag tttctccggc
     2941 aaacgcggat gatcttgctc cgttggctcg gtctcgatct ctaccttgat ctgcgagatg
     3001 accaccggac gattttcttc cacgaaaact tgggggctga cgagactccg ggtctcatcc
     3061 accacaagat cgtgagtaat cccgcttcgg taatatccct ctgcttcata gaaccggcgc
     3121 aatcgggcca ggtcggtcgc gaacgtgaca gggtcgaaga gtggccgctc ttcccagaac
     3181 cggtaccagg gacgtgattg cgtgatcatc tccgccataa cttggctatc ggaaaaatgg
     3241 tcattgcctg aaatctcgat tttatcgacc cgccactcgc ggctcgggtc gagcgcttcg
     3301 acttgaacgg cggaagcgcg gcacgccaac agtagccaca tcgccattgc agaaatcaga
     3361 gatgctgtga acttcattcc aaggttcgtc taagcttcca ttatttctgc ctcagctcaa
     3421 taggcgttcg cccagctgct gcccggaggt atcgggaact catctttata tctggagcat
     3481 gacgactcta gtgagttagg aacgaatcgt gccagggtca gcaataactt cggggtactg
     3541 taagcggatc gtattatttt accgcgactt gatgacgcgg ctggtgatcg ctcccggcac
     3601 agccgatgga gttattcgcg gtaggcaatc gtgctgagta aggcgttctc tgattgacgc
     3661 gaatcaagcc gggtatgctg gaccacgatg ggcacgtcgg actcgatgac gctggcaaaa
     3721 tcagtggccg ttggaatctt ttccggctcg ctcagatcgt tgaaccggac gtgcttggtg
     3781 cgccgtgcgc ccactgtcac cgaatacggc ccgacaggtt cccgatcaga aaagaagatg
     3841 gtgataagga cgtgagcatc ttgatccgca gtgttgagca aacacacggt ctcgtggctg
     3901 gtgaatgcgg gttcgggacc gttactccag ccgggaatgt agccctccgg tatcgcccag
     3961 agccttgctc cgattgcctt catgggttct cctcaaagcc ttgtgtaaag tgaagctttg
     4021 ttcggccgca cggattcaag gaagacttcg tcgagggaag ccgctcgatg gctagacgcc
     4081 atcttggatc tcgtgcattc ccgattcttc ggcgcagtga acgcggcaat agattgcccc
     4141 gtcggcttcc acaccgcggc ccaaatgcgg cagctgcaat gggcaggcac gcagtagctt
     4201 gtgaatgcgc attcaaagct gtcgaacgat gattccgccc gcatcgaacg atatcaaagg
     4261 ctttgtcgta atcattgccg caggcttcac attgagccat tccttcccaa tttgtgacgg
     4321 gcgttttctg ctgttcgcgc cgaccgaaga atagcgacca cgagaactgc tcaactgcat
     4381 ttccgatcag gctcacccag gctgcaaaac gcaacgggca tgccagcacc gttccgcgaa
     4441 ttcgtgcata tatgacacaa gtatgttgtc ccacagacgg aattgactta atcggttcgt
     4501 cctcggggtg agacgtcgct cgagctgtgc aaagttgaga gcggatcttg atttatcttt
     4561 gagatagtta aatgatgatc cagctatctg caaggaaagc gcccaatgtc tgccaacaaa
     4621 ccagcaattc aacctcgaac ttgtccgccg ccgctcgatg agagcatcct ggtgtcacta
     4681 taccagggga ttcctgaagc gatcgtcgtt atcgagttta aatcgcgctg tattgtgtac
     4741 tggaatgagg gtgcaacctt gttgtttggc tattcggctc aagaggtttt gcaacagacc
     4801 accgaacgat tgtacctgga caaaaactgg tttgatcgta tctatcaaac ctccattcct
     4861 gacattgagc aaaacggctg ctggcgcggt gaatggcaat atcggcgccg cgacggatcg
     4921 ctttttgccg cagaagcaac gtgcactctg ctgcaaacgg ataagggcgc ttacgttgtc
     4981 aaagtcattc gtgaggttac cgaacgcaag gaagcggaga aaattattct cgaattgaat
     5041 cagcgtttga cccagcgcgt gatcgaacaa acatccgagt tgatgtcgaa gaccgaggag
     5101 ctccagcacg aggaggcgca acgcaagcag gtcgagcaaa tcctcgggaa tttgctccgc
     5161 aacatcacta attacgcggt cctgataacc gatgccgatg gccgcatcgt tgactggaac
     5221 cgcaccgcca ctcgcattct gggccgagaa agtgacgaca tggcggatca gcggttatcc
     5281 gttttctttg acgaggaaga aggacgccag gcgttgataa aaaaggcgct tattgaggct
     5341 agtgatcaag gaacctttcg cgattaccaa cgcctggtgc gcaaagacgg ctcccggttt
     5401 tatgccagag tcattgtctt tgcgctgcgt gacgatgccg acggtttgca gggatattta
     5461 attctgttgc gcgatgactc gcaggagcga aatctgagag agaagttacg ggagaaggaa
     5521 catctggcgg cgatcggaac cgcggcatcc atgctggctc acgaaatcgg caatccgcta
     5581 aacggtattt ccacgacagt ccaactgctg gagcggttta tgattcggga gggcatcaaa
     5641 gttccgccgc acatgctagc gaccttgaag gatttgaaga gcgagatcga ccggctcact
     5701 tctctgctga acgaatttaa aaccattgcg cgacctcaaa aacttagcat tcagccggtg
     5761 cacctcgcca gcgtcatccg cgaaacgatt tcgggtctag aaatggaagg caaaggggac
     5821 gtgcagattg tctcggattg ctcgccggac ctgccgccgg tccgcggcga tgccgcaaaa
     5881 ctgaagcagg catttctgaa cctgttcaag aatgccgtcg aggccatgcc ctatggcggc
     5941 acgctgtcta tcaaaggata ttggagcggc gggtcgatct gcgtcgatat cagtgatacc
     6001 ggaatcggaa tccccgatga tctgaaagtg tttgatctgt tcagctcgac aaagcctcac
     6061 ggtactgggc taggattgtt tatcgtgcag caaattgttc tggcgcacga tggtgtggtt
     6121 tcttacgcca gtacggtagg taagggcacc accttccatt tgaccttacc tgcaagcgcg
     6181 gcaccggaag cggttgcgga catacgccgc aactagcttc cggcgacaac gttgacgcta
     6241 aaccgttctg tatcgatggg cgcagggtta ctcggagcct aggctacggt cgagctcatc
     6301 gtccttgaat ggcagccgct cgacatctcc atgtgcacct agcattcgct gtctcttctc
     6361 cagcgcggtt gcacaatcga tgcagagcgc cgctgtcggc atcgccttaa gccggccgtg
     6421 accgatatca ttgccgcatc gttcacactc gccgaaagtt cccgcatcga ttcgttcaag
     6481 agcgtcgtcg atctggcgca gcagggtctg gccgcgct
//
LOCUS       DASUTG010000334         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_646400, whole genome shotgun sequence.
ACCESSION   DASUTG010000334 DASUTG010000000
VERSION     DASUTG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646400"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..156
                     /locus_tag="VF089_07635"
     CDS             7..156
                     /locus_tag="VF089_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6173871.1"
                     /translation="MVEAPDNETVAHMSVDLGSRGTISITTLPAIPTAEFIAKLKGPK
                     QMGHK"
     gene            317..>2731
                     /locus_tag="VF089_07640"
     CDS             317..>2731
                     /locus_tag="VF089_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173872.1"
                     /translation="MNRIIRPDTWRDTEAIEWVKELRDTEAWFQSERFRQITRLHTAY
                     EVVALRGSAREDYTIARRAADKMYDYLQRLFKAKKQEITYGPFSPTGAVRAVIEGIKI
                     LYLGGWSTSARGSDSEDPGADLANYALDRVPKEGASWVRALMHQDEAQRSNRMRMSAA
                     QRKKTAAVEFSPPMIIPDGDTGHGGEHHVRNLVKKFVENKIGAIHIEDQRPGCKVCGH
                     QGQKVLVSTTEIIARLNTARLQFDVLGVPGVIVARTDSNDATAIDSLEDERDHPFVLG
                     ATNAEIVPFKNVSLAVIKKFYDRGFKEINGHLLYKISDKAYQDAQEWLRKEHLAGRVD
                     EAIAQIDKETSSLKKLRQHARRTGKGRRDAREQLAAFEVTAKRVVEETLDDVLAVIRE
                     AWADKAGLKTYGEAVAEAMQARLRSQNKLPMSVERWRKFANAMSYGEARERAKSMDVD
                     VFWDWDLPRTPEGFYPITGGRDMATARGLAMAPFCDLVWRETAKPDLEDDRIWAEAIH
                     AVFPDKLLAYNLSPSWNWDAWGFTDDQIRSFAAELGKMGYVFNFITYAGHQTEALMNG
                     RLARALKEEGVLGFVRLIQRSLRLANDPAQFPQTFVGGPWADRFRRAARGPSLTTSSM
                     GGKSTEAQHRKPVEVPTSVLERWLRMWSEHWSKQGLYKGGELSAEVKERWAGSEEMML
                     NVFDEARDKIAEIIYRVDKDRAGRKFLAVKDQNTVKKYRAHRLMTLMHFFLLHRYKID
                     LVHYVAPTDDNRLSVSRMIQNGVFREARTDDPNVIALGVDTTRAQKIFANDESLKRFI
                     TNEFRPA"
BASE COUNT          663 a          708 c          819 g          541 t
ORIGIN      
        1 gtcagtattg tcgaagcacc ggataatgag acggtcgctc atatgtcggt ggatttaggt
       61 tcacgaggga cgattagcat tactacgctg ccggcaattc ccactgcaga gtttatcgca
      121 aaactaaagg gacctaagca aatgggacac aagtgaagct gaccgaaatg ctgagtgact
      181 aagacttgat ccgttggctc tccagcgttc gccaaatgcc gcaacgttga cggcgcgtat
      241 tcgctgagtt ccagtcgtgc gcagagggct atcataccga tgccccgatc gtgaatagcc
      301 tacttgggag gttgcaatga ataggattat ccgaccggat acttggcgag ataccgaagc
      361 gatcgaatgg gtaaaggaat tgcgcgacac cgaggcttgg tttcaaagcg agcgattccg
      421 acaaattacc cgacttcaca ctgcctacga ggtggtcgcg ctgcgcggca gcgcgcgcga
      481 ggactacacg atcgctcggc gggcggccga taagatgtac gattacctgc aacgcttgtt
      541 caaagcgaaa aagcaggaaa ttacctacgg acctttctca ccgaccggag cggtccgcgc
      601 ggtgatcgag ggcatcaaga ttctctacct cggcggatgg tccacgtcgg cgcgcggttc
      661 ggactcggaa gatcctggcg ccgatctggc gaactacgcg ctggatcggg ttcccaagga
      721 aggagcctca tgggtgcgcg ccttaatgca ccaggacgaa gcacagaggt ccaatcggat
      781 gagaatgagc gcagcgcagc ggaaaaaaac cgcggctgtc gagttcagtc cgccgatgat
      841 cattcccgac ggcgacacgg gacacggagg cgagcatcat gtgcgcaacc tggtcaagaa
      901 gtttgtcgag aacaagatcg gcgccattca cattgaagat caaagaccgg gttgcaaggt
      961 ttgtggtcat caggggcaaa aggtcctggt ctccacgact gaaataattg cgcggctcaa
     1021 tacagcaagg ctgcagttcg atgtcctggg ggtacccggc gtgatcgttg cccggacaga
     1081 ctctaatgac gccaccgcga tagacagctt ggaagacgag cgcgatcatc cttttgtatt
     1141 gggcgcgacg aacgcagaga ttgttccctt taagaacgtc agtctggcgg tcatcaagaa
     1201 gttttacgat cggggcttta aggaaatcaa cggacattta ctctacaaga tctccgacaa
     1261 agcgtaccag gacgcgcagg aatggctgag aaaagaacat ctcgctggtc gagtggatga
     1321 agcgatagcg cagatcgata aagagacatc gtcgctaaaa aaacttaggc aacacgcccg
     1381 tcgcacaggc aaaggccgga gagatgcgcg tgagcagctc gcagcgtttg aagtgacagc
     1441 caagagggtc gttgaagaaa ctctcgacga tgtactggcc gttattcgcg aggcgtgggc
     1501 ggacaaggca ggcttaaaga cctatggtga agccgtcgcc gaggccatgc aagcgcggct
     1561 tcgcagccag aacaagcttc ccatgtccgt cgaacgctgg aggaaatttg ccaacgcgat
     1621 gtcctacggc gaagcccgtg aacgcgccaa gtccatggat gtcgatgttt tctgggattg
     1681 ggacttgccg aggactccgg aaggttttta tccgatcacg ggcgggcgtg acatggcaac
     1741 tgccaggggg ttggcgatgg cgccattctg cgatttggtt tggcgtgaaa cggccaagcc
     1801 ggatctggaa gacgacagga tctgggccga agccatccac gcggtctttc ccgacaagct
     1861 gctggcctac aacttgtcgc cgtcctggaa ctgggatgcc tggggtttta ccgacgatca
     1921 gatccggagc tttgccgccg agctaggcaa aatgggttac gtttttaact tcatcaccta
     1981 tgcgggccat caaaccgaag ccttgatgaa cgggcgtctg gcgcgcgcgc tgaaagaaga
     2041 aggggtgctc ggtttcgtcc ggctgatcca gcgctcgctt cgcctcgcca atgatccagc
     2101 gcaatttccg cagacgttcg tcggcgggcc gtgggcggac cgattccggc gcgcggcacg
     2161 cgggccgtcg ctgactactt catccatggg cggcaagtcc accgaggccc agcacagaaa
     2221 acccgtcgag gtgcccacca gcgttctgga aagatggctg cgcatgtgga gcgaacactg
     2281 gagcaagcag ggtctgtata aaggcggcga gctcagcgct gaagttaaag agcgctgggc
     2341 tggctccgag gaaatgatgc tgaacgtttt cgacgaggca cgcgacaaga tcgccgaaat
     2401 tatttatcga gtcgataagg acagagccgg ccgaaaattt ctcgcggtca aggatcagaa
     2461 taccgtaaag aaatatcgcg ctcaccgtct gatgacgtta atgcatttct ttctgctgca
     2521 tcgttacaaa atcgatctgg tgcattatgt ggcgccgacc gacgataacc ggttgtcggt
     2581 tagccgaatg atccagaacg gcgttttccg tgaagcgcgc accgacgatc cgaacgttat
     2641 cgctcttggc gtagacacga cgcgggcaca aaaaatcttc gccaacgatg agtctttgaa
     2701 acgttttatc accaacgagt ttaggccagc c
//
LOCUS       DASUTG010000335         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1238527, whole genome shotgun sequence.
ACCESSION   DASUTG010000335 DASUTG010000000
VERSION     DASUTG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238527"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF089_07645"
     CDS             <1..660
                     /locus_tag="VF089_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173873.1"
                     /translation="QDSQNLLSDASSTLKSLEQESAGGGPEEQNKEGGGVQSNASQQG
                     QGEGKVSEGGGGAKGELSAQLNPEMKDGKSAQGEAQGERQEKKLTDQKEQKADQSDSN
                     KPEKTTDDSTAKMKENSEGRSGKNRSDEIPKDGPPAERYRQPGEQGPDGIKGAKYVTV
                     QLPEEIAADSSGMTTSSKDGKVSKNRPKLPVSNVPMPAHVPEAPAEKQRMPLEYRDLI
                     R"
     gene            678..1658
                     /locus_tag="VF089_07650"
     CDS             678..1658
                     /locus_tag="VF089_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6173874.1"
                     /translation="MDPEKFQESFNGLEAEVRKVIVGHDDTIRKVLIAFFAGGHVLLE
                     GVPGLGKTLLVKTLSHALGVSFKRIQFTPDLMPSDIVGTQVLAEINGRREFQFKQGPI
                     FAHVVLADEINRATPKTQSAVLEAMEEKQVTVFGESHILESPFMVLATQNPIELEGTY
                     PLPEAQLDRFFFKLLVLSPTPAELREILKRTTGIETADMQDVLPRDGAKLINEMKQLL
                     RQVLIAPPIEDYVVRLVHATQPQPQNSANSPVRQYLRFGSSPRGAQSVILGAKGHALA
                     DGRVHVSYEDVENVLFPALRHRIILNFQAEAENVTADQIMAEVMKQVPRN"
     gene            complement(1730..2620)
                     /locus_tag="VF089_07655"
     CDS             complement(1730..2620)
                     /locus_tag="VF089_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6173875.1"
                     /translation="MRRTRRLLSLTILGFLLAAATVYAQANKELIAQGQYLFAIAGGC
                     ACHTVPEETYHTGGRAFPIPLGTVFSTNITQDKETGLGDWSDQQIHEAMNAGRRPDGS
                     RLLPVMPYEAYSGMAQQDLKALVAYMRTLKPIKKVTPDLKTWVPLVRSAGVPVYLRIF
                     GRWSNSPMQAPKSGVERGRYLVNHISICGDCHTPRNSIGVPNRSQYLAGASAKDGPLG
                     EEVPNITPDKETGIGDWKREDIAELLITGTKPDFDNVQGLMYEVIEGTPHGYKDMRRE
                     DALAIADYLKSVPPVKNKIR"
BASE COUNT          671 a          729 c          776 g          598 t
ORIGIN      
        1 caggatagtc aaaacctctt aagtgacgcc agcagtacgc taaagagtct tgaacaagag
       61 agcgccggcg gaggcccaga ggaacaaaac aaagaaggtg gcggtgtgca gagcaatgct
      121 tcgcagcagg gtcaaggcga aggaaaagtg agcgagggcg gcggtggtgc taagggcgag
      181 ctgtcggcgc agttaaaccc agagatgaaa gacgggaaat ccgcgcaggg agaggcgcaa
      241 ggcgagcgac aagaaaaaaa attaaccgat caaaaagagc agaaggccga tcaatcggac
      301 tccaacaagc cggaaaagac gaccgatgac tccacagcta aaatgaagga aaactcggaa
      361 gggaggtcag gtaagaacag atctgatgag atacccaagg atggaccacc ggccgagcgt
      421 tatcgtcaac ctggcgagca aggccccgat ggcatcaaag gcgccaaata cgtgaccgtg
      481 caattaccgg aagagattgc cgcggacagc agcggcatga cgacctctag caaggacggc
      541 aaagtaagca agaatcgacc aaaactaccg gtaagcaatg tgccaatgcc cgctcacgtg
      601 cctgaggctc cggcggagaa acagcgaatg ccactcgagt accgcgacct tatccgctag
      661 atcagaaagg cagttcgatg gatccagaaa agtttcagga gtcgttcaac ggtttggaag
      721 ccgaggtgcg gaaggtcatc gtcggtcatg acgatactat tcgcaaagtg ctcatcgcgt
      781 tctttgccgg cgggcacgtg ttattggagg gcgtccctgg tctggggaag actttactag
      841 tcaaaaccct cagccacgcg ttgggggttt cttttaaacg cattcaattc acgccggatc
      901 tgatgccgtc tgacatcgtc ggaacccagg tgttggcgga aattaacggc cgcagggagt
      961 tccagtttaa acaagggccg atcttcgcgc atgtggtact cgccgacgag atcaaccgcg
     1021 ccacgcccaa aacacaatcg gcggttttgg aagccatgga agaaaagcaa gtgaccgtct
     1081 tcggcgaatc ccatattctc gaatcgccct ttatggtttt ggcgacgcaa aatcccatcg
     1141 agcttgaagg tacctatcct ttaccggagg ctcagcttga ccggtttttt tttaaacttt
     1201 tggtactgtc gcccactcca gctgaattga gagagatact gaagcggact accgggatcg
     1261 aaacggcgga tatgcaagat gttttgcccc gagatggcgc caagctgatt aacgaaatga
     1321 agcagctgct gaggcaggtg ttgatcgctc cgcccatcga agattacgtc gtgcggctgg
     1381 tgcatgccac ccagccgcag ccgcaaaact cagcaaactc acctgtgagg caatacttgc
     1441 gcttcggttc gagcccgcgc ggagcgcagt cggtcatctt gggagcaaaa ggccacgctc
     1501 tcgcagacgg tcgagttcac gtcagctacg aagacgtcga gaatgttctc ttcccggcgc
     1561 tgcggcaccg gatcattcta aatttccagg ccgaagcgga gaacgttact gcagatcaaa
     1621 tcatggctga ggtcatgaag caggtgccgc gcaactagca tcctctgagt tcatctgcca
     1681 tctcatagag cgaagttgtt ctaccgactg cggagaagcg gttgctggct tatctgatct
     1741 tgttttttac gggcgggacc gatttcagat agtcggcaat agccaaggcg tcttcccggc
     1801 gcatgtcctt gtaaccgtgc ggggttccct cgatgacttc atacatgaga ccttgaacat
     1861 tgtcgaagtc cggctttgtt cctgtaatta gtagctcggc aatgtcttcg cgtttccaat
     1921 cgccgatacc tgtttctttg tcaggcgtga tgttcggcac ctcttctccg agcgggccat
     1981 ctttagcgct ggcaccggca aggtattgcg aacggttcgg aacgccgatc gagtttctcg
     2041 gcgtgtggca gtcgccgcaa atcgatatgt ggttaaccag gtaacgcccg cgttcgaccc
     2101 cgctttttgg cgcttgcatc ggcgaattcg accatcgacc gaagattcga agataaacag
     2161 gcacccccgc gctgcgcacc aaaggtaccc acgtcttcag atcgggagtc acttttttaa
     2221 tcggctttaa agtcctcata taggcgacta acgcttttag atcctgctga gccattcctg
     2281 aataggcttc gtagggcatc acgggtaaga gccggctgcc gtccggcctt ctcccggcgt
     2341 tcatggcctc atgaatttgc tggtcgctcc aatctcccag gccggtttct ttatcctggg
     2401 tgatgtttgt gctgaacacg gtaccgaggg gaatcggaaa ggcccggccg ccggtgtggt
     2461 acgtctcttc cggcacagta tgacaggcgc aaccgccggc aatggcaaat agatactggc
     2521 cttgcgcgat cagctcttta ttggcttgag catagaccgt cgctgctgcc agaagaaaac
     2581 cgagaattgt gagtgatagc agtcgcctcg tccttcgcat tcctagtcct cgtcgcatgt
     2641 gttaccgcgc tgcctcctgg cgaagcttct tatccaaaaa actcaacgta ctgtgaaaca
     2701 ctttgttgat tggcgcttgc tctccggctt gctctaataa gcgcatgagc aatccgttga
     2761 gggcatcgtg ttca
//
LOCUS       DASUTG010000336         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1979783, whole genome shotgun sequence.
ACCESSION   DASUTG010000336 DASUTG010000000
VERSION     DASUTG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979783"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VF089_07660"
     CDS             complement(<1..562)
                     /locus_tag="VF089_07660"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173876.1"
                     /translation="MVAWRARIGLIKPTHRGKSFAYWYNNAPEGVEIVPTFIGFRSEK
                     RESFLEGFRKAEEIAAQLKEVGCDIIAVSGTPPFLLKGLDFERQWAADLSAKIGLPVV
                     TPMEPHALALQALGVRSVAVATYYGQELNQAIVSYFSRFGIESELMPGLSATGESAGL
                     YTTSLRALDEVSHMDVYRHCRKALQNM"
     gene            complement(1107..2399)
                     /locus_tag="VF089_07665"
     CDS             complement(1107..2399)
                     /locus_tag="VF089_07665"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6173877.1"
                     /translation="MEFVKVWGLLALVVVAGFVVTYQYVGAPPPKVVRIATGSENGAY
                     YTFAQEYARLLASDGITLEVVPTAGSVENLELLKKGEVSLALIQGGSATKEDKERLQS
                     LGSLFLEPVWIFTRKQSGIKRFLELKGKRVSVGVAGSGTHLLATQLLLAAGITETDTQ
                     LIREETTQAIDSLSKGKIDAAFFVASPAAPIIHKLFAAPAVELLDFDRAAAYGRWFPF
                     LTPVTLSEGVIDLQQNIPSHDTPLVAASANLAARADLNASLIPAVLNAVTRVHQTGGV
                     LEHTRQFPSADFADLPLNEDARRYLINGPSFLFRWLPYGTAVLLDRLKILVLPLLALL
                     LPLFKVAPPLYTWRIRSKIYRWYAAVREVESSIQEEKVSSRAASLIERLSELDRQVAS
                     VSVPLSYIAELYHLRLHIRFLQEKLQSSASADRVRMSS"
     gene            2646..>3932
                     /locus_tag="VF089_07670"
     CDS             2646..>3932
                     /locus_tag="VF089_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173878.1"
                     /translation="MSIGYRACYSRHMAPEVKITLGRLPQFWDRNVLFFCNMHSIFYD
                     NQEEVNDLLSQIYGAHSYGSRVVSILNLLFRRGPNKIFLEAPPEPNLMDYLSGDLELS
                     LPSFAVLDHRDYQDLVTCLNNSEAKPPVEDLKAHAATWVDGFVTDRLLVEVAAGLNKR
                     TISTFEGSKNGNNKYLLYRHQLERGLPVFETLLAANHREVPAALDRLRRLGYSKAVVK
                     AQIGASGYGMIKLDTQNPQLDSMPNYLFFEGPCMVQGWLDEETPGVRRIGSPSIQMFL
                     DDEAVFLFDWTEQILSEASVHEGNMSPPPYIQEHPELKEELFRQGEIAGEWLHAQGYR
                     GTASTDFVVIEKGGQPQVIICEINARVTGATYPAVLARHFMPAGCWKMRNIQFRKSLD
                     GDQLLSLIDHAQVLYRPGKDKGIIPFNLNTDEQGKVI"
BASE COUNT          949 a         1088 c         1021 g          874 t
ORIGIN      
        1 gcatattctg taacgccttt cggcagtgac ggtagacatc catatgggat acctcatcca
       61 gagcccgcag cgaagtggtg tacagtccgg cactctcgcc ggtcgcactg agccctggca
      121 tcagctccga ctcgatgccg aaccgggaaa agtaactcac gatcgcctga ttgagctcct
      181 ggccatagta ggtggcaacc gccacactgc gcacgccgag agcctgtagc gccagcgcgt
      241 gcggttccat gggtgtgact acaggcagac ctatcttggc cgacagatcc gccgcccact
      301 gtctctcgaa atccaagcct ttgagcagaa acggcggcgt tccgctgacg gcgatgatat
      361 cgcaacccac ctctttcagt tgagccgcga tctcttcggc cttcctaaat ccttcaagaa
      421 aactctcgcg cttttcgctg cgaaagccga taaacgtcgg cacgatctct acgccttccg
      481 gcgcattgtt gtaccaatag gcgaaggact taccgcgatg cgtgggcttg atcagaccga
      541 tacgcgcgcg ccatgctacc atctaaatct cctcgtgttc ggcttacttc tagtcaagaa
      601 tcatttcaaa tgcggctatc accggcagcg gtcgatgtca aagcatcatt aagggcacac
      661 gacaacggcc atcatcgcat aaagctcgac gtaaatgtct tcttacgggc gccacgatca
      721 aaccaatcac gaatcaaaac gctgcctcac cgtatgacag cattcctcct catggcataa
      781 gagacagcta tcgcagagac tcacgtcgca gaaagagcag ttgaactcct caccatgaag
      841 aatctcgttc cggcattcgc cgcattcgat agttccattg atcagctgaa acatccgatg
      901 ttcgttgctc gagttctggt ggttagcggc gacaggtcag cagcgcttaa ttgatgagtt
      961 cgatgccggc tcactcaccc atgcggctta tcatggttca gccgattggg gcaatgaaaa
     1021 atcggcgcga tgtagagatt atcgacggtt aacagtctcg aagtatttgt ttcaaggcct
     1081 aacggatctg gaagagcacc cacaagctaa ctgctcatcc gcacccgatc ggccgatgcg
     1141 ctactctgca atttttcttg gagaaagcgg atgtgcagcc gcaggtggta taactccgca
     1201 atatacgaga gaggaactga aacagaagcc acttggcggt caagctcgct taatcgttcg
     1261 atcagtgatg ccgcacggct ggaaactttc tcttcttgaa ttgacgattc gacttcccgc
     1321 actgccgcat accatcgata aatcttggat cgaatgcgcc aggtataaag cggcggcgcg
     1381 accttaaaca agggcaggag caaagcgagc agcggtaaga caagtatttt gagacggtcc
     1441 aggagcacgg ccgtgccgta cggcagccat ctaaataaaa aagatgggcc gttgattaga
     1501 taacgacgag catcttcgtt tagagggagg tcggcgaaat ctgccgatgg aaactggcgc
     1561 gtgtgctcga gcacgccccc ggtttgatga actctggtga cggcatttaa gacggcggga
     1621 atgagactcg catttaggtc ggcgcgcgca gccaagttgg ccgaggcggc aaccaatgga
     1681 gtatcgtggg atggaatgtt ttgctgtaaa tctattactc cttcactcag agtcaccggg
     1741 gtcagaaagg gaaaccagcg gccgtaggcg gcggcgcgat caaagtcgag aagttccaca
     1801 gccggcgcgg caaagagctt atgaatgatc ggtgccgcag gcgaagcgac aaaaaaggcg
     1861 gcatcgatct tccccttcga caatgaatcg atcgcctgcg tggtctcctc gcggatcagt
     1921 tgcgtatcgg tttccgtaat gccggcagcc aataaaagct gtgttgccag caaatgagtt
     1981 ccacttccgg caacccccac cgagacacgc ttgcctttca attccagaaa tcttttgatc
     2041 ccgctctgct tgcgagtaaa gatccaaacc ggctcgagaa acaagctgcc tagagactgc
     2101 agacgttctt tatcttcttt agtcgcgctg cctccctgga taagcgcgag cgagacttca
     2161 cctttcttca agagttccag attctctacg gatcctgcgg tggggacgac ctcgagagta
     2221 atgccgtcgc ttgccagtaa acgcgcatac tcctgggcga aggtatagta ggccccgttc
     2281 tccgatcccg ttgcaatacg aacgactttc ggcggcggcg cgccgacgta ttggtaggtg
     2341 accacgaagc cagccacaac gaccaatgcc agtaggcccc aaaccttgac gaactccata
     2401 acagctgctg acacgcgtct actcataact acttggcggt cttggttgcg tacgcatcgc
     2461 gggtttcgcg cccccgcgtt ttgtccggcg atttcgattc taaagcgaga aagctaccaa
     2521 agtctagctt atgcactcaa tcctccgcgg gtatgtatcg gaatcaaggc tgtgcaacag
     2581 cctcgtcaat ctgtagcacg gcgctaacga gccacggcat gccgccgagc tgttgtgctg
     2641 gatccatgtc cattggctat cgagcgtgct actctaggca catggcgccg gaagttaaga
     2701 tcaccctagg tcggttgcca cagttctggg accgtaacgt tctgttcttc tgcaacatgc
     2761 actcgatctt ttacgataat caggaagaag ttaacgatct cttaagtcaa atttacggcg
     2821 cccactccta cggcagcaga gtcgtttcca tcctaaactt gctgtttaga cgaggaccta
     2881 acaagatttt tttggaagcg cctcccgaac ccaacctgat ggattacctt tccggcgatc
     2941 tggaattgtc tctgcctagt tttgccgtct tggatcatcg cgactatcaa gatctcgtca
     3001 catgcctaaa caattctgaa gccaagccgc cggtcgaaga tttgaaagcg catgcggcaa
     3061 cctgggtgga tggttttgtc acggaccggc tactggtcga agttgcggcg ggattgaaca
     3121 agcgaaccat tagtacgttt gaagggagca aaaacggcaa caataaatat cttctgtacc
     3181 gccatcagct ggaacgagga ttgccggtct tcgaaactct acttgcggcc aatcaccgag
     3241 aagtcccagc cgcgctcgat cgcttgagac ggttgggcta ctcaaaggca gttgtcaaag
     3301 cccagatcgg cgcttccggc tatgggatga tcaagctcga tacccagaac ccgcagctgg
     3361 atagcatgcc caactatctc ttcttcgaag gcccctgtat ggtgcaaggc tggctggatg
     3421 aagaaacgcc tggagtccgg cgcatcggct ctcccagcat tcagatgttt ttagatgatg
     3481 aggctgtttt tttattcgac tggaccgaac agattctcag cgaggccagc gttcatgaag
     3541 gcaacatgtc cccgcctccc tatattcaag aacatcctga gctcaaggaa gagcttttcc
     3601 gacagggcga aattgccgga gaatggctgc acgctcaggg ctatcgcggt acggccagta
     3661 ccgacttcgt ggtgatcgaa aaaggcggac aacctcaggt catcatctgc gagatcaatg
     3721 cacgggtgac gggggcaacc tatcccgcag tgctggcgcg acactttatg cctgccggat
     3781 gttggaagat gcgaaacatt cagttccgca aatcactcga tggcgatcag cttctctccc
     3841 tcatcgatca cgcacaggtg ctctaccggc ccggcaagga caaaggaatc attcctttca
     3901 atcttaacac tgacgaacaa ggcaaagtca tc
//
LOCUS       DASUTG010000337         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_53781, whole genome shotgun sequence.
ACCESSION   DASUTG010000337 DASUTG010000000
VERSION     DASUTG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53781"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..1375)
                     /locus_tag="VF089_07675"
     CDS             complement(464..1375)
                     /locus_tag="VF089_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6173879.1"
                     /translation="MIPMMNTYDTIRTSLHFNKFVVGELLFVEYKCPIEQEAAGVWTP
                     MDYFVHVLSGKKTWRTTDGTWTAEKGQTLFFKKGAAVVYQDFKDDFCVLVFFVSDNFI
                     RDVVREVSGQLNGQPHGYALQKAAILVNGDVTLAAYFESMLAYFTGTSKPAETLLTLK
                     LKELIVSILLGGKNPELAGYFQSLLKSDAPVLPQIMEANFSYNLALEDFARLSHRSLS
                     TFKRDFRKYYKQSPGKWLLQKRLDYSAVLLKNPALNVSQVALDCGFEDLSHFSRAFKE
                     KFGTSPAHYRKETPGRGDSQLREADLL"
     gene            1761..2636
                     /locus_tag="VF089_07680"
     CDS             1761..2636
                     /locus_tag="VF089_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6173880.1"
                     /translation="MRIAIGLPSRVSSASGAVMLEWITRADRGPFSSVVVTDRVVSQA
                     LEPLTVLAVAAGATRRVGLMTSVVIGPTRETTLLARQAATIDVLSGGRLTLGIGIGVR
                     GNDYLATGFDFHRRGRRAEEQLPILRRLWSGEALSGEVGAIGPRPSRADGPELLIGGY
                     VPAIVQRIAKWGDGYMAPGGGEPEALLRTWRQIESAWQQAGRTGKPRWVGASYYTLGP
                     KAQDHASRYINANYGYNPDLAARRLSTLPASDSAVQESIKRQADMGVDEFILRPCADE
                     LDQMDRLAEIAARTV"
     gene            2727..>3569
                     /locus_tag="VF089_07685"
     CDS             2727..>3569
                     /locus_tag="VF089_07685"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6173881.1"
                     /translation="MHLPVNPPVLPMLAKRVSELPRGSNWMFEPKWDGFRALVFRDGD
                     EVLLQSRDEKSLNRYFPELPAPLRTQLPARCVLDGEIVIAKNGALDFDALQLRIHPAA
                     SRVKLLSQEIPASIVLFDILAEGDRDLRGLAFRDRRRILESLLSSAAPPIHLTPVTGE
                     LVVAQDWFRRFEGAGLDGVIAKPDSGTYEANKRVMLKIKHERECDCVVAGFRWHKKGE
                     RNAVGSLLLGLFDDTGTLQHVGVCASFTDKKRRELVEFLAPYGKDALAAHPWKGWAEQ
                     EATGG"
BASE COUNT          782 a         1010 c          989 g          788 t
ORIGIN      
        1 gttcgaatag ttcataattg cctccttaaa ggtttattct gctgccgaag ataaagcggg
       61 gcagagaaag atgctttgtt taagaggcca gatgtttgac tgagcggctc tttctcatgc
      121 gtgttgctga aaaagtccac ctgcttcgtt gcgctccgat ggactcgctc cgaaatactg
      181 catgagtatg cctacgcgct cgcccattct cacgtgcctt ctagctcttt ctgagcaacc
      241 tacgagagcc tgctaaaaat caataggctt ccgcgctctc ggccgactgc gcatcaacgt
      301 gcaagagcac gcctccgctt atcgatcatg cgcgtcgctt gtatgcgcaa acttctgacc
      361 attttgaatg caatgattaa acatcgtacc gcctgggcag aactcaagct tcagacccct
      421 taatttgaac acagttgctc gcctctggat ctttttgaac agcctacaag aggtctgctt
      481 cacgaagctg cgagtcgccg cgaccgggag tttctttacg ataatgtgcc ggggaggtgc
      541 cgaatttttc tttgaaagcc cggctgaaat gcgacaggtc ttcgaagccg caatccaagg
      601 cgacttgcga aacgttgagc gcgggattct ttaacagcac ggctgaatag tccagtcgct
      661 tctgcaataa ccacttgccc ggcgactgct tgtaatactt gcggaagtcc cgtttgaacg
      721 tcgacaagct gcgatgggat aatctcgcga aatcttccag cgccaggttg taagaaaaat
      781 tcgcctccat gatttgcggc agcacaggcg cgtcgctttt gagcagcgat tgaaagtaac
      841 cggcgagctc cgggttcttg cctcccaaaa gtatgctcac aatcaattcc ttgagtttga
      901 gtgtcagcaa cgtttcggcc ggcttcgacg tcccagtgaa ataggccagc atcgattcaa
      961 aataggccgc caaagtcaca tcgccgttta ctaaaatggc ggctttttgg agcgcgtagc
     1021 catggggctg gccgttcaac tgaccggaaa cttctctgac aacatcgcgg atgaaattat
     1081 ccgaaacgaa aaagactaaa acgcagaaat cgtctttgaa atcctggtag acgacggcag
     1141 cgcctttttt gaaaaacaaa gtctgcccct tttctgccgt ccaggtccca tcggtagtgc
     1201 gccaggtttt tttgccgctc agcacatgaa caaaataatc cattggcgtc cacacgccgg
     1261 cggcttcctg ttcaatcggg cacttgtact cgacgaaaag aagctctccg accacgaact
     1321 tattgaagtg caaactcgtg cgaattgtgt cgtacgtatt catcatcggg atcatatagc
     1381 ctccgcctga aagttttgta tctcctccag ggcttccagc gcaccatctt gctattttgc
     1441 gaggcgcttt ccaagctcct cgcaggcagc ggcaaccagc caagcttgac gccaagcagg
     1501 ttaggattgt atccccattg tagaaaggat tttgaggtct gcttacgcag ggccgacttc
     1561 aaaatgggga attagcctag cctgagaatt cctcaacgcc tgctaaagac gccgaagtca
     1621 cgcagggacc attttaaaat attatgcgta tccgctggaa tgttgtggcc actccggtcg
     1681 aaaaaaatct ctcgcagtga gtgatcgaaa tagccgctgg cctcggacaa ttcgagcgac
     1741 attcaaatct cggaggaatc atgcgcatcg ccataggctt accaagtcga gtatcctccg
     1801 cctccggcgc agtgatgctc gaatggatca cccgcgcgga ccgcgggccc ttttcgagtg
     1861 tcgtggtcac cgatcgagta gtttcccagg cactcgagcc gcttacggtg ctggcggtgg
     1921 cggctggagc gacgcggcgc gtgggtttga tgaccagcgt cgtgattgga ccgacgcgtg
     1981 aaaccacgct cctggcccgt caggccgcga ccatcgatgt gctctcaggc ggccggctca
     2041 ctctgggtat cggcattggc gtgcgcggga acgattacct tgcaactggg tttgactttc
     2101 atcgccgcgg tcgccgcgct gaagagcagt tgccgatact gcgccgcttg tggtctggcg
     2161 aggcgctatc cggtgaagtc ggagctatcg ggccacggcc atctcgcgct gatggtcccg
     2221 agctgttgat cggcggttat gttcccgcca tcgtgcagcg catcgccaag tggggcgacg
     2281 gttacatggc gccaggcggt ggcgagccgg aagccctgct caggacatgg cggcagatcg
     2341 agagcgcatg gcaacaagcc ggacgcaccg gaaagccgcg atgggtgggc gcgagctatt
     2401 atacgctcgg tcctaaggct caggatcatg cgagccgcta tatcaatgcg aactacggct
     2461 acaatcccga ccttgcggcg cggcgcctga gtactttgcc tgcttcagat agcgccgtgc
     2521 aagagtcgat caagcgtcag gctgacatgg gagtcgatga attcattctg cggccatgcg
     2581 ccgacgagtt ggatcagatg gaccgcctcg ccgaaatcgc cgcccgcacg gtgtgagaca
     2641 gacggtatcc tggcgatcaa tcgccaaagt acccggtgat cttcgatctt aacgactgga
     2701 acatgaaacc cgcaagagac tgacgtatgc atcttcccgt aaacccgcca gttttgccga
     2761 tgcttgccaa acgagtcagc gagttacccc gaggctcaaa ctggatgttc gaaccgaaat
     2821 gggatggatt ccgcgccctg gtgtttcgcg atggagacga ggtcctgctc cagagccgcg
     2881 atgaaaaatc gctgaaccgc tacttcccgg aattgcccgc gccgctccga acgcaattgc
     2941 ccgctcgttg cgtgcttgac ggcgaaatcg tcatcgccaa gaacggcgcg ctcgacttcg
     3001 atgcgctgca gctgcgcatt catcccgcag cgtcgcgggt aaagcttctt tcacaagaaa
     3061 tcccggcatc catcgtgcta ttcgacatcc tcgccgaggg agaccgggat ctgcgcggct
     3121 tggcgttcag agatcgtcgc cggattctgg aatcgctgct ttcgtccgca gcgccgccaa
     3181 tacacctcac cccagtaacg ggggaattgg tcgtggcgca ggattggttt cgtcgcttcg
     3241 agggcgctgg tcttgacggc gtcattgcca agcccgattc gggaacctac gaagccaaca
     3301 agcgcgtgat gctcaaaata aagcacgagc gcgagtgcga ttgtgtcgtt gccggttttc
     3361 gctggcataa aaaaggcgag cgcaacgcag tcggctcact cctgctcggt cttttcgacg
     3421 acaccggcac gctgcagcac gtcggcgtct gcgccagctt caccgacaag aagcggcgcg
     3481 agctcgtgga atttctcgcc ccatacggca aggacgcgct agcggctcat ccctggaaag
     3541 gttgggccga acaagaggca accggcggt
//
LOCUS       DASUTG010000338         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1833173, whole genome shotgun sequence.
ACCESSION   DASUTG010000338 DASUTG010000000
VERSION     DASUTG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833173"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1139)
                     /locus_tag="VF089_07690"
     CDS             complement(231..1139)
                     /locus_tag="VF089_07690"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6173882.1"
                     /translation="MGYLPKEGVSFELIEFQSGARTVQGGLFGPDPKLFHKPDTAVVL
                     VHGVESYWYSGPTMFLGCYLAEAGYAALAYNGVHSGESFRTSEFEGAVEEVDAAVAFV
                     QARGFKNIYLAGHSLGTPIVEYYQGDTPSPLVKALGVYGPHSNIPSVTKESLLGPDLY
                     EKFTAECRSLVSSGRGEEIKLLPFREGKVIITSAKTFLSYRDVDTSKAAVEKMIANIK
                     VPLLIVYDGADNIQGKGSITRRETIAARIKENAVNCPRADIVVIPSRPGNSPFQAHLF
                     LNNERTVTHETVNWLTSVGLAPARLR"
     gene            1460..1897
                     /locus_tag="VF089_07695"
     CDS             1460..1897
                     /locus_tag="VF089_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173883.1"
                     /translation="MGLLKIEGITHWSIPVNDLDESERFYGDLLGLKPLGRLGNSGMS
                     CFNVADQHILLCQRAESVDKSHVEEENVHHSFTVSPETLEQACKLFAEKKVAIDRLYY
                     RAKGYFPGRELYFFDPSGNRLELRDPTWKAGMPEPTFEDLARS"
     gene            complement(2160..>2482)
                     /locus_tag="VF089_07700"
     CDS             complement(2160..>2482)
                     /locus_tag="VF089_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6173884.1"
                     /translation="AFRLGLVQKICNPDKLIDDCVELCTTINRNGRLIAAHTTEYIED
                     RLLYEIQGYEKGFEVHREYYAHLRQSKDYDEGSAAFTQRRKPEFSQEYYDEKTKLPGV
                     PETK"
BASE COUNT          582 a          651 c          658 g          591 t
ORIGIN      
        1 gggacaaatt tggcgggtgt aaaatgaggc tatgtgcctc acggtatctg agttccgttt
       61 cgattttcgg gctcaaaatg aattgcgacg tttggactcg acaaagctgg cgtcaccaca
      121 gttctcgaca cttcgacagt attcagctga cgaacaggta cactcgcttt cggaatgtgt
      181 cgccgaatac ggttcacaca gtcagctgac cgacgcctta cgatctctgc tcatcgtaac
      241 cgggccggcg cgagaccgac gctcgtcaac cagttcaccg tctcgtgtgt cactgttcgc
      301 tcattgttca aaaacagatg ggcctgaaaa ggggagttgc cggggcgaga gggaatcacg
      361 acaatatcgg ctctaggaca gttcacggca ttttctttga tccgggccgc gatagtttca
      421 cgtcgggtga tgctcccttt tccctggatg ttgtcggcac catcataaac gatcaacagc
      481 ggaaccttga tattcgcaat catcttttcc acggcggctt tgctggtatc tacgtcgcgg
      541 tagctgagaa aggtcttggc cgaggtgatg atcactttgc cttcacggaa tggcagaagt
      601 ttaatctctt cgcctctgcc gctcgaaacc aagctgcgac actctgcggt aaacttctca
      661 tagaggtcgg gaccgagcag agactccttt gtgacgcttg gaatattgct gtgaggaccg
      721 taaacgccca gcgctttaac taaaggactt ggtgtgtccc cttggtagta ttcgacgatc
      781 ggagtgccca ggctatggcc ggcgagatag atattcttga aaccgcgcgc ttgaacaaaa
      841 gcgacggcag catcaacttc ctctaccgcg ccctcgaatt ccgatgttcg aaagctctct
      901 ccggagtgga caccattgta ggcgagcgcc gcataaccag cctcggcgag atagcatccg
      961 agaaacatcg ttggtccgga ataccagtac gattcgactc catgcaccaa cacgacggca
     1021 gtgtcgggct tatgaaatag ctttgggtcc gggccgaata agcctccctg cacagtcctg
     1081 gcgccgcttt gaaattcaat cagctcaaag ctcacgccct cctttggcaa atagcccatt
     1141 cgtaaactgc cttcctttct aattattttt tccgaagcga agagcatcga tcagagcgcg
     1201 gttggattgt cgcgtggcag gaggcgcaaa cgctgacgcg ctaaggcgaa gcattctccc
     1261 acatcgtccg ttcgtaccat gatcacagca agagcgaaac tgcgagccag catcggtccc
     1321 ccagcgatct gttcgtgtgc gtttttccgg cagcctgttt gcctagattg acagaaagcc
     1381 gttgctggcg atagaagcgg actgagtgat cagcaaacgg gttgcgatct taaagcaatg
     1441 taaagggagg cagatctata tgggactact caaaatcgaa ggaattacgc actggtccat
     1501 tccagttaac gatctggacg agtcggaaag attttacggc gatctcctcg ggctaaaacc
     1561 gctggggcgg ctcggcaatt ccggcatgtc ctgcttcaat gtcgccgatc aacacattct
     1621 actgtgccag cgcgcagaga gcgtcgacaa atcccacgtc gaggaggaaa acgtgcacca
     1681 ttctttcacg gtgagcccgg aaaccctcga gcaggcgtgt aagttatttg ctgaaaagaa
     1741 ggttgccatc gatcgcctgt actatcgggc aaaagggtat ttccctggga gggaactcta
     1801 tttcttcgat cccagcggca atcgtctcga gctgcgtgat cccacctgga aggccggcat
     1861 gccggaacct actttcgagg acctcgcccg ctcgtaaaat cgcggattag ttcaagccac
     1921 tcgagcagtt cgagtcgttc aaaaaaaacg tcaagcacag agcctcttga tgagaagttt
     1981 attcggcttg agtagtagag agaggcagtt acggtcgagc ggcgaagctg agcagtgaag
     2041 aatcaaaatt gacaaagaat tgagcaaaaa attgggaacc gtttgaacgg cttgaacgat
     2101 ttggacctta gctacgggcc aaatcgctca agccggctcg gatgataatc gagccgcagc
     2161 tacttggtct ccggcactcc cggcagttta gtcttttcat cgtagtattc ctggctaaac
     2221 tcaggtttgc gtctttgggt gaacgcggcc gatccttcat cgtagtcttt gctctggcgc
     2281 aggtgggcgt aatattctcg gtgcacttcg aatccttttt cgtacccctg aatttcgtag
     2341 agcagtcgat cttcgatata ctccgtcgtg tgggcggcga tcagccggcc attgcggtta
     2401 atcgtcgtac acaactcgac gcagtcatcg atgagcttgt cgggattgca aattttctgc
     2461 accagtccta accggaacgc cg
//
LOCUS       DASUTG010000339         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_54273, whole genome shotgun sequence.
ACCESSION   DASUTG010000339 DASUTG010000000
VERSION     DASUTG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54273"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF089_07705"
     CDS             complement(<1..373)
                     /locus_tag="VF089_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173885.1"
                     /translation="MPRSDTLSQSNMEKPLWFPLSRIVRLRAFQALGHREYRLLWYSQ
                     VFASLGVWMDQVTRGWLIYELTDSALQLGMVRGIQAIPFLFLSPVAGSVADRYPRKFL
                     VVTAQFANGFIFTATAALILFG"
     gene            941..1969
                     /locus_tag="VF089_07710"
     CDS             941..1969
                     /locus_tag="VF089_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173886.1"
                     /translation="MKRFVCALLFLLTSVTNVQAQAPFYQGKTIRIIVGNLAGDAYDL
                     WARIFAQHMGKYIPGNPTFIVQNMTGAGGIIAANYVYSVAKPDGLTLGTFGPSMYFDQ
                     LAKRPEVQFDWARFSWIGTPEQTEFILIMRTDTPYQSIEDVRKAAQPPKCGATGTSTS
                     GYYMPKFLDEGLGIKFNIVSGYPGGGEIDLGIERGELQCRNLTISTYFAREPFLTWHK
                     KGFVRPILQTAKKRDRLVPDVPTIYELMDQLKTPEDTRRVATVLLAPAVFGRPMVATP
                     GVPPDRLKMLRDAYEQSLKDPALLEDLKKRRWSVDAVSGEELAKLAKEVVSQPPDVVE
                     RMMKLLAN"
     gene            complement(2100..>2412)
                     /locus_tag="VF089_07715"
     CDS             complement(2100..>2412)
                     /locus_tag="VF089_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HEX6173887.1"
                     /translation="AQAYLTEAGNVRHEHEYKVWRDIKLPDGKILIPGVVSHATPVIE
                     HPELVADRILAFANIVGRENIIAGTDCGLGGRLHPQLAWAKLRALSEGAALASKKLWG
                     S"
BASE COUNT          546 a          689 c          684 g          493 t
ORIGIN      
        1 gtccgaagag aatcagcgct gcggtggccg tgaaaatgaa gccattggca aactgcgccg
       61 tgaccaccag aaactttcgc ggatagcggt cggcgacgct gccggccacc ggcgataaga
      121 acagaaacgg tatcgcctga atgccgcgca ccattcccag ttgcagcgcc gaatcggtaa
      181 gctcgtagat caaccatccc cgggtcacct gatccatcca cacgccaaga gaggcaaaaa
      241 cctggctgta ccatagcagg cggtactccc gatggccgag cgcctggaag gcacgcaaac
      301 ggacaatccg gctaagcggg aaccaaagcg gcttctccat gttgctctgt gagagtgtat
      361 cggatcgcgg catcggcgcc gcgttcgggc ttgacggcga ttgcaaaatt tcgatcggcc
      421 gaaggagacg agcagtctcg ctgacttgaa gcccgtgtta aggatcccta ctcttctatc
      481 tcaccgcacg aaaaagttcc agccggagaa accgaacacc tgcaggatcg agtgctggtc
      541 agccgatcgc aaccgttctg gcagcgcgcc aaaactcgca tgatagacta cattgaaccg
      601 catgcaggta agttgtgaag cacagatcaa gagtgagtat gaaaagctcg caccgcagtt
      661 taaactcgcc aaaagcctca tcagagtgcg aatgcagaag gctggacaca ggcgcaacta
      721 gcgcggcggg tcgtcgcatc ccggcctgga tcatcggtct cggtatcccc cggaacatac
      781 aacgaggggg ttatccattc tgatttctga accctagtaa ggaatcatgc tgtttttgat
      841 cggagcattc ccttgacagc ttaactgtac ccgccttatg atccgcggca tttccgcatt
      901 taaagatagt tggttaacgc ccaggcggtc cggaggtgca atgaaacgct tcgtttgcgc
      961 gctgttattt ctattgacgt ccgtaactaa cgttcaagct caggcacctt tttaccaagg
     1021 aaaaactatc cgcatcatcg tcggtaatct cgccggcgat gcctacgatt tgtgggcgag
     1081 aatcttcgcc cagcacatgg gcaagtacat cccgggaaat cccacgttca tcgtgcagaa
     1141 catgaccgga gccggcggaa tcatcgctgc caattacgtt tactccgtgg ccaagccgga
     1201 cgggctcaca ctgggcacat tcggaccctc gatgtacttc gaccagctcg ccaagcgtcc
     1261 tgaggtgcag ttcgattggg cgagattctc gtggatcggc acgccggagc aaaccgaatt
     1321 catcctgatc atgaggactg acacgccgta ccaatcgatt gaggatgtgc gcaaggcggc
     1381 acagccgccc aagtgcggcg ccaccggtac cagcacttcg ggttattaca tgccgaaatt
     1441 tttggacgaa ggcttgggga tcaagttcaa tattgtcagc ggctatccag gcggcggcga
     1501 gatcgatctc ggcatcgagc gcggcgagct ccaatgccgc aatttgacga tcagcaccta
     1561 cttcgcgcgc gagccgtttc tgacgtggca caagaagggc tttgtccggc cgatcctgca
     1621 gaccgccaag aaaagagatc gtctggttcc tgatgttccg accatctatg aattgatgga
     1681 ccaactcaaa acaccggagg acactcggag agtggctacg gtactgctcg ccccagcggt
     1741 tttcgggcga cccatggtgg cgacgccggg cgtgccgccg gatcggttaa aaatgttgcg
     1801 cgacgcctat gaacagtcgc tcaaggatcc ggctctgctg gaagatttga agaagcggcg
     1861 ctggtcggtg gatgcggtca gcggtgagga gttggccaag ctcgccaaag aagtggtctc
     1921 acaaccgccg gatgtcgtcg aacggatgat gaagctcttg gcaaattagc ttttgatatc
     1981 aatgcagccg tcgcttcagg atacgcgggg attaattcta cggatcgaat tgcccccctc
     2041 ctgccccgcg cgaccacatc ctcagggtca ctcgatgaac tgcggatcgg ctagatcgat
     2101 taactgcccc acaacttctt gctcgctagc gcagcgcctt cggataacgc gcgcagcttt
     2161 gcccaggcga gctgcgggtg caggcgcccg ccgagcccgc agtcggttcc ggctatgata
     2221 ttctcccggc caacgatatt ggcaaaggcg agaatccgat ccgctaccag ctcgggatgt
     2281 tcgatgacgg gcgttgcgtg gctcaccacg ccgggaatga gaatctttcc gtccggcaat
     2341 ttgatgtccc gccagacttt gtattcgtgc tcgtggcgca cgttgccggc ttcggttaag
     2401 taggcctgcg ct
//
LOCUS       DASUTG010000340         5623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_54563, whole genome shotgun sequence.
ACCESSION   DASUTG010000340 DASUTG010000000
VERSION     DASUTG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54563"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1782)
                     /gene="murJ"
                     /locus_tag="VF089_07720"
     CDS             complement(121..1782)
                     /gene="murJ"
                     /locus_tag="VF089_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX6173888.1"
                     /translation="MNEEVGKLTRAAGVVGFFTLLSRVSGLFRDVVIGFLFGAQGAAD
                     AYFVAFRIPNLLRRLTAEGALTVAFIPVFTNYLAKEGKAEAVKVTQIVFTFLAIFLGM
                     ITFLGIVFARPLTHFFAPGFVQEPEKFEMAVLLTRWMFPYIFFVSLVALAMGVLNALR
                     HFMAPAMSPVLFNLCNIVCAILFYPVLDEPILGLAFGVLLGGAAQLLFQIPYLAKQGI
                     QLRFDFNLKHPGLVRLLYLMGPAAFGAAVYQINVLVSTMLASFLPSGSVSYLYYADRF
                     LEFPVGIFAIALGTAALPSFSTLVVAGNVKELRATLAYALRIVNFICLPATAGLAVLA
                     VPLFAIFFQRGAFDATATLNSAQALIFFSFGLWSISATKLVAPVFYAMEDTKTPVFVG
                     ILAFVVNLIASLALMGEINLQSDSAGMMTRMIASASLRIGLVSLGHGGLALATTISSL
                     CNFFVLLLILHRRLEGIPLREVAQSFLRNLISTLLMALPLFWIVGQVDWTGTGIATYL
                     KALILACLISLGISLYLALSYLLRSTEWQIVDQFRRALQSRLYRG"
     gene            complement(2160..2759)
                     /locus_tag="VF089_07725"
     CDS             complement(2160..2759)
                     /locus_tag="VF089_07725"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HEX6173889.1"
                     /translation="MRRASALTALGYLWAALPIAGCGYSFAGRSEAFPDDIRTVYIEP
                     FINQTRNVGLEKELTSALRSEFYRRGELRVVDTVDQADGIIAGVVRSFESHVASVNRR
                     DEVLQYEAAMIVDASLRRREPNEIIWRSQGTRLTDIYGGSRAAVVTTSSEFKTGTLNA
                     SDVRRLTDIQLTETENRAVRGQLVERFARELHQRLMEMF"
     gene            complement(2788..5379)
                     /gene="leuS"
                     /locus_tag="VF089_07730"
     CDS             complement(2788..5379)
                     /gene="leuS"
                     /locus_tag="VF089_07730"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX6173890.1"
                     /translation="MEPRYQPKDIEQKWQKFWEENKSFRASEEQGKPKYYVLEMFPYP
                     SGRIHMGHVRNYSIGDVVARYKRMRGYNVLHPMGWDAFGLPAENAAIEREIHPERWTQ
                     ENILYMKAQLKRMGLSYDWSREIATCQPEYYRWNQWIFLQFYKRGLAYKKSSLVNWCP
                     SCETVLANEQVIEGLCWRCESIVTQKELDQWFFRITEYAEELLRDLERLPGWPEKVLL
                     MQRNWIGKSVGAEIIFPLVGRAEPLKVFTTRPDTVFGATFISLAVEHPLALKVSQGLP
                     QEEAVREFIATIQQRKSTGQGVGEGEKDGVFTGAYATNPFTGENIPIYLANFVLMEYG
                     TGAVMAVPAHDQRDFEFARKYGLPIRVVIQPEGSILRAEDLTDAYVNEGVMVNCGAFS
                     GLGNIDGKEKIAEHLEKEGWGKKAIRYRLRDWGVSRQRYWGTPIPIIYCEHCGIMPVA
                     EEQLPVTLPIDVPFTGKGGSPLAGSKTFIELQCPQCSAAARRETDTMDTFVDSSWYYV
                     RYASPTLITAPFDSKQAEYWMAVDQYIGGVEHAVLHLLYARFFTKALRDIGLAKIDEP
                     FTNLLTQGMVCKETYRCPDHNWLLPSELVGSEKDGWRCPHCTRPVEKGRVEKMSKSKK
                     NIVDPEELVSTYGADTARLFTLFAAPPEKDLEWSDQGVEGAYRFLNRLWRFILHNREL
                     LAGESDSGQLNGSAELRELHRMIHRTIKKVTDDIDGRFHFNTAIAAIMELFNALSIAA
                     SQDGGRVAAPVIRSGMETIIQLLYPFVPHLMSELWQRLDKSQPLDQIPWPEYSPEALE
                     EEQLLIVLQVNGKVRGKMTVPADIQRDQVETDALAEPRVKAFIGGKKVQRIVYVPRRL
                     VNIVLEG"
     gene            complement(5384..>5623)
                     /gene="nusB"
                     /locus_tag="VF089_07735"
     CDS             complement(5384..>5623)
                     /gene="nusB"
                     /locus_tag="VF089_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX6173891.1"
                     /translation="IDRLIQECTENWKLARMAKVDLLILRMAAYELLFCPDIPLNVSL
                     DEAIEMGKRFGSGDSGSFINGVLDQLAHTEIAKPH"
BASE COUNT         1450 a         1560 c         1391 g         1222 t
ORIGIN      
        1 cataaactgt tgataaccgc atgacaaccg gcctgtgccg cagaaagatc gcggaatcta
       61 gcagaatgcc cactttgaca gcagtcttcc actggtcctt gtacccttaa gccgcgagag
      121 tcagcctcgg tacaggcgcg actgcagcgc gcgccggaat tgatctacaa tctgccattc
      181 agtactcctc agcagatagc tgagcgcgag atacaatgat atcccgagac taatcaggca
      241 agcgagaatc agcgctttca gataagtggc gattccggtg cctgtccagt cgacttgacc
      301 cacaatccag aaaagcggca gcgccataag caaggtactg atgagattcc gcaggaagga
      361 ctgggcgacc tctcgcagtg gaattccttc caggcgcctg tgcagaatta gcagcaagac
      421 aaagaaattg cataatgagg agatcgttgt cgccaacgca agtccgccgt gacccaatga
      481 aactagcccg atacgaagcg acgccgaagc gatcattcgg gtcatcattc cggcggaatc
      541 actttggaga ttgatctcac ccatcagcgc aagactggca atcaggttga ccacaaaggc
      601 taggattcca acaaacaccg gggttttagt gtcctccata gcgtagaata ctggcgcgac
      661 caacttcgtg gctgagatgc tccagagtcc aaatgagaag aaaataagcg cttgagccga
      721 gttcagcgta gcagtagcat cgaatgcgcc tcgttgaaaa aaaatcgcga atagcggaac
      781 cgccaggaca gctaatcctg ccgtggccgg caaacaaatg aaattgacga ttctgagggc
      841 atacgccagc gtcgcgcgaa gttctttgac attaccggca acaaccaatg ttgaaaaact
      901 gggcagggct gccgtgccca gagctatggc aaaaattccg accgggaact ctaaaaatcg
      961 atctgcataa tacaggtaag aaacgcttcc gctcggcaga aaggaagcca gcatagtgct
     1021 caccagaacg ttaatctgat acaccgccgc gccgaatgcc gccggcccca ttagatacaa
     1081 caaacgaacc aacccgggat gttttagatt aaaatcaaat cgcaactgaa taccttgttt
     1141 cgccagataa ggtatctgaa atagaagttg tgccgctcca ccgagtagaa ctccgaaggc
     1201 caagccaagg atcggttcgt ccagcactgg gtagaataga attgcgcaga cgatattgca
     1261 gagattgaaa agaacgggtg acatcgctgg cgccatgaaa tggcgcagcg cgttcagtac
     1321 tcccatggcc aaagcaacca ggctgacgaa aaaaatgtat ggaaacatcc aacgggtcag
     1381 caataccgcc atttcaaatt tctcaggctc ctggacaaaa ccgggggcga agaagtgggt
     1441 aagagggcgg gcaaaaacga ttcccaaaaa tgtaatcatc cctagaaata tagctaaaaa
     1501 agtaaataca atttgcgtga ctttaacggc ctcagccttt ccctctttcg ccaagtagtt
     1561 ggtaaacacc ggaataaatg caacggtcaa agcgccttcc gctgtcagcc gccgcaacag
     1621 attggggatt ctaaaggcga cgaagtaggc atcggcggca ccctgcgcgc cgaagagaaa
     1681 gccgataaca acatcgcgaa acaagcccga gacccggctg agcaaggtga agaagcctac
     1741 caccccggcg gcccgtgtta acttcccgac ttcctcgttc aaattgactc cgataacccg
     1801 aggtggtaag taaatcgaaa catttgtagg gaggctagct ttggcagatc atccgtcggt
     1861 tattaagaga cacaggcaaa gtctgaaacg aaaggcaaga aaccaggaga cgagatccaa
     1921 ggtcagaacc ttgatcaaaa aagcgcgcca agcagtctca agtcagagcg ccgattctcc
     1981 ggccgctaac atcaaagccg tcaataaagc attgggtaag gctgtgagca agggaatcat
     2041 caatcgaaat actgcatcgc gttggctatc ccgattggcg cgggcggccg ctcgcacctc
     2101 tccatctgta tagcaatctt atcccgcttt gtatcgaagc gccgacggac ccatcggcgt
     2161 taaaacattt ccatcaatct ttgatgcagc tctcgcgcaa aacgttcgac tagttgtccg
     2221 cgcacggcgc ggttttcagt ctcagttaat tggatatcgg tcagccgccg aacgtccgac
     2281 gcgttcaacg ttccggtttt gaattccgac gacgttgtca ctaccgccgc tcgggacccg
     2341 ccatagatat ccgtcaggcg cgtgccttgg cttcgccaga tgatttcatt tggctctcgc
     2401 cgacgcagcg acgcatcgac gatcatcgcg gcttcatatt ggagcacttc atccctgcgg
     2461 ttaacggaag cgacatggct ctcaaacgaa cgcactactc ccgcgatgat cccatcagct
     2521 tgatcaacag tatcaacgac acggagttcc ccgcgccggt aaaattcgct gcgcagcgcc
     2581 gaagttagtt ctttctcaag accgacgttg cgcgtttggt taatgaacgg ctcgatgtaa
     2641 accgtacgaa tatcgtccgg aaacgcctcg ctccgtccgg caaatgaata accgcagcca
     2701 gcgattggca gagcggccca aagatagccc agggcagtta acgcgcttgc ccgtctcatg
     2761 gctcgccgca gagcatattt caaaggttca tccctccaag acaatgttga ccagccgccg
     2821 cggtacgtaa acgatccgct ggaccttttt cccgccgatg aaagctttaa ccctcggctc
     2881 ggcgagagcg tcagtttcaa cttgatctct ctgaatatcg gcagggacgg tcattttgcc
     2941 gcgcactttg ccgttgactt gcagaacgat aagaagttgc tcttcttcga gcgcctccgg
     3001 tgaatactcc ggccagggga tttgatccag tggctggctt ttgtccaggc gttgccagag
     3061 ttcgctcatc aagtgcggca cgaacggata gagaagctgg ataatcgttt ccatgccgct
     3121 ccggataacc ggcgcagcca cgcggcctcc gtcttggctg gcagcgatac tgagtgcgtt
     3181 aaagagctcc ataatagccg caatagcggt attaaagtga aaccttccat cgatgtcatc
     3241 cgtgaccttc ttgatcgtcc gatggatcat gcgatgcagc tcgcgcagtt cagcggatcc
     3301 gtttagctgc cccgaatcgc tttcgccagc aagcagctct cggttgtgca aaataaaacg
     3361 ccacagccga ttcaagaatc gatacgcgcc ctctactccc tgatcgctcc attcaagatc
     3421 cttctcggga ggagccgcaa acaacgtgaa tagccgcgcc gtgtcggcgc catacgttga
     3481 gacgagttct tccggatcga cgatgttttt cttcgacttg gacatctttt caacgcggcc
     3541 cttctcaacc ggccgtgtgc aatgagggca gcgccatcca tccttttctg aaccgacaag
     3601 ttcgctcggc aggagccaat tatgatccgg gcagcgatag gtttctttgc aaaccattcc
     3661 ttgggtaaga agattggtaa aaggctcatc gattttggcc agcccaatat ccctcaaagc
     3721 tttggtaaaa aaacgagcat agagcaggtg aagcacggca tgctcgacac cgccgatgta
     3781 ttgatccacg gccatccagt actcggcctg cttagaatcg aagggagcag taatcaaagt
     3841 cggagaagcg taacgcacgt aataccagga cgaatcaacg aaagtatcca tcgtgtcggt
     3901 ttcccgacgc gcggcggcac tgcattgagg gcactgaagc tcgatgaaag tcttactccc
     3961 ggccagagga gagccgccct tgccggtaaa cggcacatca attggaagcg taacaggcaa
     4021 ctgctcttca gccacaggca tgatgccaca atgctcacaa taaataatcg gaatcggcgt
     4081 tccccaatag cgttgccgcg atacccccca gtctcgcagc cggtagcgaa tcgccttttt
     4141 accccatccc tctttctcca gatgctcggc aatcttttct tttccatcga tgttgcctaa
     4201 cccgctgaat gctccgcaat tcaccattac gccttcatta acatacgcat cggtcaaatc
     4261 ttcagcgcgc agaatgctgc cctcgggttg aatcaccact cggatcggca agccatactt
     4321 gcgggcaaat tcaaaatccc tttgatcgtg ggcaggcacc gccatcacgg cgccggtccc
     4381 gtactccatc agcacgaagt tggcgagata aatcggaatg ttttcgccgg tgaacggatt
     4441 ggtagcatac gctccagtga aaactccgtc tttttcacct tctccaacgc cctggccggt
     4501 tgatttccgt tgctgtattg tggcgataaa ttctcgaaca gcctcttctt gcggcagccc
     4561 ttgggaaacc tttaaagcca aaggatgctc aaccgcgagc gatataaagg tcgcgccaaa
     4621 tacggtatca ggacgggtgg tgaaaacttt cagcggttct gctcgcccga ccagcggaaa
     4681 gatgatttct gcgccgacgg atttgccgat ccaattgcgc tgcatgagca gcactttctc
     4741 aggccaacca ggtagtcgtt cgagatctcg caacagctcc tcggcgtact cggtgatgcg
     4801 aaaaaaccat tgatccagtt ctttctgagt cactatcgat tcacagcgcc aacaaaggcc
     4861 ttcaatcacc tgttcgttag cgagcaccgt ctcgcaggaa gggcaccagt tgactaaaga
     4921 actcttctta tatgctagcc cgcgtttata gaactgcaga aatatccatt gattccagcg
     4981 gtaatattca ggctggcaag tggcgatctc acgcgaccaa tcgtaggaca ggcccatacg
     5041 cttgagctgc gctttcatgt acaggatgtt ttcttgagtc cacctttcag gatgaatttc
     5101 ccgttcaatg gcagcattct cagccgggag gccgaaggca tcccatccca tgggatgcag
     5161 aacgttgtat ccgcgcatgc gtttataacg agcaaccacg tcaccgatgg aataattacg
     5221 cacatggccc atgtgaatac gccccgaagg ataaggaaac atctcaagca cgtaatactt
     5281 aggtttcccc tgctcttcgg aagcccggaa ggatttattt tcctcccaaa acttctgcca
     5341 cttctgttct atatctttgg gttggtaacg cggttccatg ctgtcagtga ggtttggcga
     5401 tctctgtatg cgccagttga tccaacacac cgttgataaa agagcctgaa tcgccgctcc
     5461 cgaaccgttt gcccatctcg atagcctcat ccagactgac attgagggga atgtccggac
     5521 aaaagagcaa ttcgtatgcg gccatccgga gaattagcag gtccactttg gccatccgcg
     5581 cgagcttcca gttttccgtg cactcctgga tcaaccgatc gat
//
LOCUS       DASUTG010000341         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_795210, whole genome shotgun sequence.
ACCESSION   DASUTG010000341 DASUTG010000000
VERSION     DASUTG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795210"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VF089_07740"
     CDS             complement(<1..1582)
                     /locus_tag="VF089_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6173892.1"
                     /translation="MSAAHAPSNGYDYIVVGSGAGGGTVAARLAEAGRTVLLLEAGGD
                     PLSLGDTRLPEDYQVPAFHAFASENPALRWDFFVRHYRDDERQKKDSKFTAERDGVLY
                     PRAGALGGCTSHNAMILVYPHNQDWDDIADLTGDPSWRAENMRRYFERLEDCRHRPPY
                     RWLQKILRLNPTRHGFSGWLTTERRDPRLALGDRDMMGMIKHSALKAFKELGDPLTQV
                     RWSIKSQHDPNDWRLVQSNSFGVRYTPLTTRNQARIGSRERILDVAKKYPRKLHVESH
                     ALVTRVLFDEDNRALGVEYLKGERLYRAHSKPSSGPGETRQAFASRETILCGGAFNTP
                     QLLMLSGVGAGEDLQSHGIKVRVDLPGVGKNLQDRYEVSVVNRMTQDWNVLAAAKFDT
                     SDPQYREWAADRKGVYATAGAALVVVKKSQPSRTVPDLFCLGFIGKFEGYFPGYSKLI
                     SAHHNYLSWVVLKAHTRNTAGAVSLRSADPRDTPEVNFRYFDEGSDGSGEDLDGVVEG
                     IKFVRKMTAALRTEKVISE"
     gene            complement(1609..>3286)
                     /locus_tag="VF089_07745"
     CDS             complement(1609..>3286)
                     /locus_tag="VF089_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173893.1"
                     /translation="VKPPADPENPKWENLNSNPGTQYFWVDRILFWEKDEANFIQQLL
                     HTSLPGTLDTSFISTDYINNPRTMNAVYSVAARLKAAERWGEETLAGGGVKNKQFQDF
                     KQTAALAQYHKPPDTVRTPRILKDGADSVGVLGALNRVFINIGLFSEEWLLHFNALVG
                     GKKITPIKIEDAEKNSSYWNATVAQTPDVALFFLKTAQPDLLKNAPGGEQYLTKDQAQ
                     LARGKTVFAETCARCHSSKIPAAPAGVDERDWEQYWKWTKTDDFKKKMTEMVMADDFL
                     VDNYLSTDRRIPVTLLQTNACSPLATNALGGNIWDNYSSQTYKDLPSVGKITVHDPID
                     GSPREYEMPGNGRGYTRVPSLISLWSTAPFLLNNSVGKFNPSPSVEARIESFNDSIEK
                     MLWPEKRDKDPILGDKVPGFVYRTTATSYVKLAPGFLPASLNHLLSWNDLGARWLPWL
                     FSEEGVQIGPIPKGTPVNLLTNIDLESSKIDLAKLLLRMKADLKAVEGKSEQEAAEVF
                     KTLVPDLLQVSKCPDFVVNRGHYFGTSLLKEEPPLSDDDKRALIEFLKTF"
BASE COUNT          681 a          946 c          891 g          768 t
ORIGIN      
        1 cctctgaaat aaccttctct gttctgaggg cggctgtcat tttgcgcaca aacttgatgc
       61 cttcgacaac gccgtcgaga tcctctccac tcccgtcact accctcgtcg aagtagcgaa
      121 aattaacttc cggagtatct ctgggatctg ccgagcgcaa actcaccgct cctgccgtat
      181 tgcgcgtatg tgctttcaac actacccaac tcaagtagtt gtgatgcgct gaaataagtt
      241 ttgagtatcc cgggaagtag ccttcaaatt tgccgataaa gccgaggcag aacagatccg
      301 gaacggtacg actcggctgg gattttttaa ccaccaccag cgccgcgccg gcagtggcgt
      361 acacaccttt ccgatctgcg gcccattctc gatattgcgg atcgctggta tcgaactttg
      421 ccgctgccaa tacattccaa tcctgcgtca tgcgattgac gacgctcacc tcataccggt
      481 cctgaaggtt tttgccgacg ccgggaaggt caactcgcac tttaataccg tgagactgca
      541 aatcctctcc cgcgcctacg ccggagagca tgagcaactg gggggtatta aaagctccgc
      601 cgcataagat tgtctctcgc gaagcaaacg cctggcgtgt ttctcctggt ccgctgctgg
      661 gtttgctatg ggcgcggtac agacgctcgc ctttgaggta ctcgactcct aatgcacgat
      721 tatcttcgtc gaacagcact cgagtcacga gagcgtgaga ctcgacatgc agcttacgag
      781 ggtatttctt ggccacatcc aagatgcgct cgcgactgcc gatgcgcgcc tgattgcgcg
      841 tcgtcagagg tgtgtagcgc accccgaacg agttggactg caccaagcgc cagtcattgg
      901 ggtcgtgctg cgatttgatt gaccatcgaa cttgtgtcag cggatctccc aattctttga
      961 atgccttcag cgccgaatgc ttgatcatgc ccatcatgtc tcgatcgccg agcgccaacc
     1021 tgggatctct tctctcggtg gtcagccagc cgctgaaacc gtggcgggtc ggattcaatc
     1081 tcaagatttt ctgcaaccag cgatagggag gccgatgacg gcagtcctcg agacgctcga
     1141 agtaccggcg catgttctca gctcgccagg aaggatcgcc cgtcaggtcg gcgatatcgt
     1201 cccaatcctg gttgtgcgga tagaccagaa tcatcgcatt atggctcgtg caaccgccca
     1261 acgcacccgc ccgcgggtaa aggacgccgt ctctctcggc agtaaattta gagtccttct
     1321 tttggcgctc gtcgtcacgg taatggcgca cgaagaaatc ccagcgcaac gcgggattct
     1381 cggaggcaaa ggcgtgaaag gcaggaacct ggtaatcttc cggtaaacgt gtgtcgccta
     1441 gactgagagg atcgccgccc gcttccagga gcaataccgt gcgtccagct tcggccaatc
     1501 gcgcggcgac cgtgccgccg ccggcgcccg atccgacgac gatgtaatca tacccgttcg
     1561 atggcgcatg tgcggcagac attcttattg gagttcgccg ggagaacgct agaacgtctt
     1621 caagaattcg atcaacgccc gtttgtcgtc gtcgctcagc ggcggctctt ccttcagcaa
     1681 actggtgccg aagtagtggc cccggttgac gacgaaatcc ggacacttgc tgacctgcaa
     1741 aagatcgggc acgagggttt taaacacctc ggccgcttcc tgttcgctct tgccctccac
     1801 cgccttgaga tcggctttca tccttaataa cagcttggcc agatcgattt tgctactctc
     1861 caaatctata ttggtaagca gattcaccgg agtacccttc ggaatgggac cgatctgcac
     1921 cccctcctcg ctaaacagcc acggcaacca gcgcgccccc aagtcattcc agctgagaag
     1981 atggttcaaa gaagcgggca agaacccggg cgcgagtttg acatagctgg tcgcggtcgt
     2041 gcggtaaacg aagcccggca ccttgtcgcc gagaatcggg tctttgtcac gcttctccgg
     2101 ccacagcatt ttttctatgg aatcgttaaa cgactcgatg cgcgcttcca ccgatggcga
     2161 gggattgaac ttgccgaccg aattgttgag cagaaaaggc gccgtggacc acaggctgat
     2221 cagcgacggg acgcgggtat agccgcgccc gttgccgggc atctcgtatt cccgcgggct
     2281 gccgtcgatc gggtcatgca cggtgatctt gcctacggat ggcaggtctt tataggtttg
     2341 cgatgagtaa ttgtcccaga tattgccgcc cagagcattg gtcgccagcg gactgcacgc
     2401 gttggtctgc agcaaggtaa cggggatgcg gcggtcggtg gacagataat tgtcgacgag
     2461 aaaatcatcc gccatcacca tctcagtcat ttttttcttg aagtcgtcgg tcttggtcca
     2521 tttccagtat tgctcccagt cccgttcatc cacaccggcc ggcgctgcgg gaattttgct
     2581 ggagtggcag cgcgcgcagg tctccgcgaa gaccgtctta ccgcgggcca actgagcttg
     2641 atccttagtt agatattgct cgccgcccgg agcattcttg agcagatccg gctgcgctgt
     2701 ttttagaaaa aataacgcaa cgtccggagt ctgtgcgacg gttgcgttcc agtagctgga
     2761 atttttctcg gcatcctcga tttttatcgg ggtaattttc ttgccgccca ccaacgcatt
     2821 aaaatgcagc agccactctt cgctgaacag tccaatattg atgaaaaccc ggttcagcgc
     2881 gccaagcacg ccgacggaat cggcgccgtc tttcaagata cgcggggtgc ggaccgtatc
     2941 gggtggttta tggtactgcg ccaaagccgc ggtttgcttg aagtcttgga actgcttgtt
     3001 cttgactcct cctccggcga gtgtttcctc gccccaacgt tcggccgctt tcaagcgtgc
     3061 cgccacactg taaaccgcat tcatcgtgcg cggattattg atgtagtcgg tggaaataaa
     3121 cgaggtatct aaagtacccg gcagggacgt atggagcagt tgctggatga aattggcttc
     3181 gtccttttcc cagaacagaa tccgatccac ccagaaatat tgcgtgccgg gatttgagtt
     3241 caggttttcc cacttggggt tttccggatc agcaggcggt ttgacc
//
LOCUS       DASUTG010000342         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_203072, whole genome shotgun sequence.
ACCESSION   DASUTG010000342 DASUTG010000000
VERSION     DASUTG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203072"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..1301
                     /locus_tag="VF089_07750"
     CDS             510..1301
                     /locus_tag="VF089_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6173894.1"
                     /translation="MAERVWDRFLTEQDKATLVGRAERKIGFGERPALLLIDLYRWVF
                     GDKPEPVVEAIKTWPGSCGLAGWNALPHIQKLLGKAREVGIPVIHITGMDGVAIEPWA
                     FRRDKTKRADMNQEQLDRQRRKFDIVDQVKPIPGETVLKKVSPSAFWGTPLIAHLNFL
                     GVDTVLTCGESTSGCVRASVVDGTTNRLRMIVAEECVFDRHEACHAINLFDMNQKYAD
                     VLPLEEILKYLDAWRAEKAGQVGYANDSIAYLKDLALGEDFKGLR"
     gene            complement(1317..2513)
                     /locus_tag="VF089_07755"
     CDS             complement(1317..2513)
                     /locus_tag="VF089_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173895.1"
                     /translation="MKLSPLAFFSLAAVCSIAGSSGAAMLAPFFMSARGYSVALAGIP
                     LVVNGAGRVCSDLSSGVLASYFSSGMLIIAASLFALLISTLSILFLDITAVFLVAFTL
                     LGITEAMFALSIRKIAFDTSEPGQQGRAQGQVASALGIGFAIGPLLGGFAGRIFGPDA
                     LFLVYAAPQLLSLLFLLLGGAHRSGKISREPSVRILREGQKLLSSPSFLASCLAIFQS
                     FLFLVGVTRVAFPFLAVNQRGLGLDWVGTMVSISRLADTAGRYSGGRLCDRIRPERVI
                     LLGILIGIPMFVLQVYGTGVTALLIPLCVMTMGFGYTNVGATTFALQSAGSAGKGLAL
                     GLSRASTSIGQMLGPLLAGFLVEKFGYDQGFETMAVISAVVLLVVWRGLNKQPAGAVI
                     DAGRSD"
     gene            2630..2944
                     /gene="glpE"
                     /locus_tag="VF089_07760"
     CDS             2630..2944
                     /gene="glpE"
                     /locus_tag="VF089_07760"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004718408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEX6173896.1"
                     /translation="MEIPQIEIHQAKERLDQQQAVFVDIRDPASYRAAHIPGALHLHD
                     GNVQEFVQNTDKDNPVVVYCYHGNSSLGAAAYFLENGFKNVASMSGGFEAWRQVYDHE
                     AE"
     gene            2985..>3323
                     /locus_tag="VF089_07765"
     CDS             2985..>3323
                     /locus_tag="VF089_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173897.1"
                     /translation="MGVILGSGEFRYEVIDCWGKLPDGWTFHEVASVGVDKNDNVYCF
                     TRGAHPVIVFDRHGDFLRSWGEGRFKRAHGVTMGPDDTIYLTDDGDHTVRKCTLDGEV
                     LLTLGIPGKPA"
BASE COUNT          851 a          850 c          972 g          650 t
ORIGIN      
        1 taaatgaggc gccgatatct ttaagctgtt cggcagcggt gaggtaacta gctcttgcag
       61 gagataccag tacgagccag aagagtattg aaatgacgat gagttgcact gtgactgccg
      121 cgcagtataa cgcaggtcac agcttaaggc aaaaaaaacc tcggccttcg ttgatatttt
      181 ttcaacgaaa gctaacgagg attcaagcgg gagtcagcta cgatatggcc gaggcgccac
      241 tgaggaaaga tggcgcaatc tttgtgccac tgaggtaatc tcctgactcc tagggattcg
      301 agaataggtg atgtatgaca ttgccttcaa aggacaacct cttcacggaa tcagaagaga
      361 aatacctaca gcgactcact ggagttgagc ctggcactcc acgtgatctg tcgaagataa
      421 ccggtttctt gacgctctca cgaagtgagt gatagtttgc caatcatacc tcacaatgac
      481 cgacacctaa ccagcaaagg agatcttcta tggctgaacg agtatgggat cgttttttaa
      541 ccgaacagga taaagccaca ttggtcggaa gagctgaacg caaaattggc ttcggcgaaa
      601 ggccggcttt gctgctgatt gatctctacc gctgggtttt cggcgataag cccgaaccgg
      661 tggtagaggc gattaaaacc tggccgggaa gttgcgggct ggcgggatgg aacgcgttgc
      721 cacatattca gaagctcctc gggaaggcgc gcgaagtcgg cattccggtt attcacatca
      781 ccgggatgga cggcgtcgcc atcgaacctt gggcatttcg ccgcgataag actaaacgag
      841 ctgacatgaa ccaggagcag ctcgatcgac agcggcgaaa attcgacatc gttgatcagg
      901 tgaaaccgat tccgggagag acggtgttaa aaaaagtctc accgagtgct ttctggggta
      961 cgccgctaat cgcccacctg aactttctcg gggtagacac ggtgctgaca tgcggcgaaa
     1021 gcaccagcgg ctgtgtgcgc gccagcgttg tcgacggaac gaccaatcgg ttgcgcatga
     1081 ttgttgcgga agaatgtgtt tttgaccggc acgaggcctg tcacgctatc aatcttttcg
     1141 acatgaacca gaagtatgcc gacgtgttgc cgttggagga aattctcaaa tacctcgatg
     1201 cctggcgcgc tgagaaggcc gggcaggtgg gctatgccaa tgacagcatc gcctatctga
     1261 aagatcttgc cctgggtgaa gatttcaagg gactgcgcta gcagatctgg aattgattaa
     1321 tcagaccggc cggcatcgat aacggcgccg gccggttgtt tgttcaggcc tcgccagacg
     1381 accaacagca ccacggcgga aatgactgcc atggtttcga atccctgatc gtaaccgaac
     1441 ttttccacca ggaacccggc taagagcggc ccgagcatct gtccgatcga agtggacgcg
     1501 cggctaagcc cgagagcgag ccctttgccg gcgctgccgg cggattgcaa ggcgaaagtg
     1561 gtggcgccga cattggtgta gccgaacccc atcgtcatta cgcacaatgg aatgagtagt
     1621 gccgtcactc cggtgccgta aacctgcaag acaaacattg gaatgccgat cagaataccc
     1681 agcagaatca cgcgctcggg cctgatacga tcgcaaagcc gaccgccgct gtaccgcccg
     1741 gctgtatcgg ccaagcggga gattgaaacc atcgttccga cccaatcaag ccccaacccg
     1801 cgttgattga cggccaagaa gggaaaggcc acgcgggtca ctcccaccag gaaaagaaac
     1861 gactgaaata tcgccaggca ggaggcaaga aaggaaggag agctgagcag cttctgacct
     1921 tcgcgcagga ttcggactga aggttcccgg ctgatcttgc ctgaacgatg cgcgccgcca
     1981 agaagaagaa acaacaagct taagagttgc ggcgccgcat agaccaggaa caaagcgtcc
     2041 ggtccaaaaa tccttccggc aaatccgccg agcagcggac ctatggcgaa gccgataccg
     2101 agagccgatg ccacctgccc ttgggcgcgg ccttgttgtc cgggttcgga ggtgtcgaag
     2161 gcgatcttgc gaatcgatag ggcaaacatt gcttcggtga tgcctaagag tgtaaaagca
     2221 acgagaaaga cggcggtgat atcgagaaag agaatgctga gcgtgctgat tagtagcgca
     2281 aacagcgagg cggcaatgat cagcataccg gagctgaaat aggatgccag aactcccgat
     2341 gacaaatctg agcagacgcg cccggcgcca ttgaccacca gtggaatccc ggcaagcgcg
     2401 acggaatagc cacgcgccga catgaaaaaa ggtgcgagca ttgccgcgcc cgacgaaccc
     2461 gcaatcgagc acaccgcagc gagggaaaaa aatgccagcg gggagagttt catggcgccg
     2521 gattatagca gctgagttgg cggcggcata cggaacggcg aaagattccg acattgtgga
     2581 cagtgccgat gcgccttgat aaaaggacag cgttaggcga ggaaatcaaa tggagattcc
     2641 acagatagaa attcaccagg cgaaagagcg gctcgatcag caacaggcgg tgttcgtcga
     2701 tatccgcgat ccggcctctt atcgagcggc gcacattccg ggagcgctgc atctacacga
     2761 cggcaacgtg caagagttcg ttcagaatac ggacaaagac aatccggtag tggtttactg
     2821 ctatcacggg aacagcagtc tcggcgccgc agcctacttt ctcgagaacg gttttaaaaa
     2881 tgtcgccagc atgagcggcg gtttcgaggc atggcgtcaa gtctacgatc atgaggcgga
     2941 atgatccgtc gcttgattat tgatggcgat caggaactag atctatgggt gtcattttag
     3001 gtagcggaga atttcgctac gaagtaatcg attgctgggg caagcttccg gacggctgga
     3061 cttttcatga agtcgcctcg gtcggcgtcg acaagaacga caatgtttac tgctttaccc
     3121 ggggcgcgca tccggtgatc gttttcgacc gccacggtga ttttttacgt tcctggggcg
     3181 agggcagatt caagcgtgcc cacggcgtaa ccatgggtcc ggatgacacg atctatctga
     3241 ccgatgacgg tgatcacacg gtgcgcaaat gcaccttgga cggcgaagtc cttcttacct
     3301 tgggtattcc gggcaagccc gct
//
LOCUS       DASUTG010000343         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_795371, whole genome shotgun sequence.
ACCESSION   DASUTG010000343 DASUTG010000000
VERSION     DASUTG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795371"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VF089_07770"
     CDS             <1..1139
                     /locus_tag="VF089_07770"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173898.1"
                     /translation="DQVTRGWLIYELTDSALQLGMVRGIQAIPFLFLSPVAGSVADRY
                     PRKFLVVTAQFANGFIFTATAALILFGLIRPWHVYLTALLMACVQVFLQPARAAMISD
                     TVPPENLTNAIGLNAVVFNMARSTGPALAGVLISVFSTGVAYGAQGLLFLLATLWTTQ
                     MRSGRRAAAARRSHGGGNESFARSIVSGWQFSWRKDEVRSSLLIVMVASLFMVPFSTL
                     LPIFARDILEVGAQGQGLLLASMGVGALGSALIIATFGDRMPRGIMMFAGVTLYGVLV
                     VLFSASPWFSLSMVLMAIIGLCHVSSHALVQTVIQSYSPPEFRGRAIAIFHMNQILLL
                     LGGLVIGALSAVMGAPWAAAALSIAGTLCMIALYIFDPRARKIR"
     gene            1470..2513
                     /locus_tag="VF089_07775"
     CDS             1470..2513
                     /locus_tag="VF089_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173899.1"
                     /translation="MKKTLCAILATICTLAVAAGFPPHSEAQSKKVVVQLAWVIGGLH
                     AGFFVAKEKGFYASKGLDVAINRGFGSGDTAKVVATGTAHFGEVNVPTAIISRGKELP
                     LTVVGVIIGKAPESILSFADKGIRKPKDVEGKTFAEATGAAIMVTWPAFAKLAGIDTS
                     KTTHIPVEAAAKPAVFFSGQVDWVPGFRPGFDEPLIIRARKEGKKLHFIRWEDFGWKV
                     YGNGLVTNNDLLKREPKTVANFVAGTMEGYAYAIAHPDEALDITLKANPELDRESARL
                     SLLFAIDAMMTKASQERGLGYMEEDRMAFQIKLMSDLMKFTLPKGVYTNQFIQKKSVT
                     VPPALAAELKKLP"
     gene            2515..>2993
                     /locus_tag="VF089_07780"
     CDS             2515..>2993
                     /locus_tag="VF089_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6173900.1"
                     /translation="MPESIRVERLAKAYRSRDGSEVEALRDVSFTVDRGEFVSIVGQS
                     GCGKSSLLKILAGLLSKTSGVISIEDREVDGPRRDIGLMFQKPLLLEWRRVLDNVLLP
                     VEIYRLDRKEYEEKARRLLGTAGLADFLTKYPFELSGGMQQRVALCRALVAEPSLLL"
BASE COUNT          591 a          843 c          874 g          685 t
ORIGIN      
        1 tggatcaggt gacccgggga tggttaatct acgagcttac tgattcggcg ctgcaattgg
       61 gaatggtgcg cggcattcag gcgataccgt ttctgttctt atcgccggtg gccggtagcg
      121 ttgccgaccg ctatccgcga aagtttctgg tggtcacggc gcagttcgcc aacggcttca
      181 ttttcacggc taccgcagcg ctgatcctct tcgggctcat tcgaccctgg catgtctatc
      241 tgaccgccct tctgatggcc tgcgtgcagg tgtttcttca gccggcccgc gccgccatga
      301 tctccgacac cgtgccaccg gaaaatctga ccaacgccat cggcctcaat gcggtcgttt
      361 tcaacatggc gcgcagcacc gggccggcgt tggcgggcgt gctgatatcg gttttcagta
      421 ccggcgtggc gtacggcgcc cagggattgt tatttcttct ggcgaccctc tggaccacac
      481 aaatgagatc gggacggcgc gccgctgccg ctcgaagaag ccatggcggc ggaaacgagt
      541 ccttcgcgcg cagcatcgtg agcggatggc aattttcctg gcgcaaagat gaagtccgca
      601 gcagtctcct gatcgtgatg gtcgcgtcgc tgttcatggt gccgttttcc accttgttgc
      661 cgatttttgc gcgcgacatt ctcgaagtcg gcgcgcaagg acaggggctg cttctggcgt
      721 ccatgggcgt cggagcgctc ggcagcgccc tgatcatcgc caccttcggc gaccgcatgc
      781 cgcgcggtat catgatgttc gccggcgtca cgctttatgg cgtgctcgtg gtgctgtttt
      841 cggcttcgcc ctggttctcg ctgtcgatgg tattgatggc gatcatcggc ctctgccatg
      901 tcagctccca tgcgctggtg caaaccgtca tccagagcta ctcaccgccc gagtttcgcg
      961 gccgggcaat cgccatcttc cacatgaatc aaatcctgct actgctcggc ggcctggtga
     1021 ttggcgcatt gtcggccgtc atgggcgcgc cttgggcagc ggcggcgctg agtatcgcgg
     1081 gcactctctg catgattgcg ctttatattt tcgacccgcg ggcgcgaaaa atacggtgat
     1141 accaaagcaa gagaattaag tattgaagtg atggaacgct gaaaatgaag ccgtcgaggg
     1201 cggtactcgc ggtggatcgc acatttctcg aaatcctcat agtttagctg cgaatcatct
     1261 gcttcttgtt tctctttgat cgacccgctg agatcttcgt cgcgatcttt gttttgactt
     1321 caccatagct gcgtcttcga actgcgcttt cagagtggcc ggcctgatca ggtgcgcaat
     1381 cttctttgac ttgactggaa tcagggtgat agcttacgcc caaagaaagt ttgtcagttc
     1441 ggagaataca aggcgaagag gaggatgtcg tgaaaaaaac tttatgcgca atcctggcta
     1501 cgatttgcac actcgccgta gcagcgggtt ttccgccaca ctccgaagcg cagagcaaga
     1561 aggtcgtggt tcagctggcc tgggtcatcg gcgggctgca tgcaggcttt ttcgtcgcca
     1621 aagaaaaagg cttctacgcc tccaaaggct tggacgtggc gatcaaccgg ggcttcggca
     1681 gcggcgacac cgccaaggta gtcgcaaccg ggaccgcaca ctttggtgaa gtgaatgtgc
     1741 cgacggcaat catctcgcgt ggcaaagaac tgcccttaac cgttgtcggt gtcatcatcg
     1801 gcaaggctcc ggagagcatc ctgtcatttg cagacaaagg aatccgcaag cccaaagatg
     1861 ttgaaggaaa gactttcgcg gaggcaaccg gagcggccat catggtgacc tggcccgcct
     1921 tcgccaagtt ggcgggaatc gacaccagca aaacgaccca tatcccagtg gaggccgctg
     1981 ccaagcccgc agtgtttttc agcggccagg tcgattgggt tcccgggttt cgccctggct
     2041 ttgatgaacc gttgatcatt cgcgcccgaa aggaagggaa gaagctccat tttattcgtt
     2101 gggaagactt tggctggaag gtttacggca acggtctggt taccaacaac gacctgctca
     2161 agcgcgagcc gaaaacggtg gctaattttg tcgccggcac tatggagggc tatgcttacg
     2221 ccattgcgca tcccgatgag gcgctcgata tcacgctcaa agcgaaccct gaactggacc
     2281 gggagtccgc ccgtctttcg cttcttttcg ctatcgacgc catgatgacg aaagcttcgc
     2341 aggaacgagg cttgggctac atggaagagg accggatggc ctttcagatc aagctcatgt
     2401 ccgatctcat gaagttcact ttgccaaagg gcgtatacac caatcaattc attcaaaaga
     2461 aatcggttac agtgcctccc gcgcttgcag cggaattgaa aaaactaccc tagcatgccg
     2521 gagtcaatcc gcgtcgaacg gcttgccaag gcctaccgat cgagggatgg gagtgaagtc
     2581 gaggcgcttc gggacgtttc cttcaccgtg gaccgcggag aatttgtttc gatcgtcggc
     2641 caaagcggct gcggcaagtc gagcttgctc aaaattcttg ccggccttct ttccaaaacc
     2701 agcggcgtga tctctatcga agatcgcgag gtggacggac ctcgccgcga catcggcctc
     2761 atgttccaga agccgctcct gttggaatgg cgccgggtgc tggataacgt cttgctgccg
     2821 gtggagattt accgcctcga tcggaaagaa tatgaggaaa aagcgcgccg gctcctcggc
     2881 accgccggtc tagcggattt cctcacgaag tatccgtttg agctttccgg cggcatgcag
     2941 caacgggttg ccttgtgccg cgcgctcgtg gccgagcctt cgctccttct gat
//
LOCUS       DASUTG010000344         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_55176, whole genome shotgun sequence.
ACCESSION   DASUTG010000344 DASUTG010000000
VERSION     DASUTG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55176"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..500)
                     /locus_tag="VF089_07785"
                     /pseudo
     CDS             complement(285..500)
                     /locus_tag="VF089_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264889.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(586..1095)
                     /locus_tag="VF089_07790"
     CDS             complement(586..1095)
                     /locus_tag="VF089_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6173901.1"
                     /translation="MEAPDKSENRVSDQAESGAAESEAEIARLSEELRREHDTLLRTL
                     ADFENYRRRTERDRASAAQSGKRDLILSLLEVLDDFDRALHHMGDAPVSVVQGMQAIQ
                     RNLLAVLERNGITRFNSVGETFAPRLHDAIGTVPSDGRNPGTITEELQSGYKWGDEVL
                     RPARVRVAQ"
     gene            complement(1137..1502)
                     /locus_tag="VF089_07795"
     CDS             complement(1137..1502)
                     /locus_tag="VF089_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX6173902.1"
                     /translation="MVADAQAHSDEDRKRREAIEARNSADSMAYQVERQVSELGDRVP
                     MNERARAEQLISQIRELVKANSSDVARLRQLTSDLQQVGYGLSSAASSQDNTGGQRPG
                     GRPDTSGGDDVIDAEFKES"
     gene            complement(1857..>2187)
                     /locus_tag="VF089_07800"
     CDS             complement(1857..>2187)
                     /locus_tag="VF089_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6173903.1"
                     /translation="PPVDIYDSPDGLVLLADLPGVAPEDLKVRLEENTLTIQASPKHS
                     VDGEATYKEFELVNFFRQFELSEEVDQEKISASLSHGVLKLQLPRAEKAKPREIPVKV
                     QGERSGA"
BASE COUNT          415 a          670 c          579 g          523 t
ORIGIN      
        1 cttaccaccc aggattgctg ctcgccggca gctaattgcc gatccaggct ctttgctttc
       61 agtcgttctc ttggtcattg gtcgcgtgaa tcagctgacc ctgttggact tcgaggttgc
      121 cctggcgctc actggtctga ggcaccgtcc aaaaactgtc aaaggttttc ttaatcaaag
      181 ccaccgatgc ccccgaagac atcctccata tcgccgaagt caaccccgag cttcgcgctg
      241 ccagccagga ggcggcctga actcgtcgcc gccttccaat taggcccgag ggcgtcgtaa
      301 cacttgcgct tttccggatc cgacaggacc tcgtaggctt cgctaagttc ctttgaactt
      361 ttcttctgct gatttgtctc cgggattgac gtcggggtgg tacttgcgcg ccggcttgcg
      421 gtacgaagac ttgatctcat cttcggacgc ggttttattg ccgccggcac ctgatagtag
      481 tctctgaatt gaactgccat ttaagtatca aagagcgtgc ttcgagccgg tttggacact
      541 ccgttgatcc aaatactcgt ctaagtcgac ggagagcgcc aactttcatt gcgcgacgcg
      601 cacgcgcgcg ggcctgagca cttcatcgcc ccacttgtaa ccgctctgga gttcttcggt
      661 gatcgtcccc gggttccttc cgtcgcttgg aaccgtgccg atggcatcgt gcaaacgcgg
      721 agcgaaagtc tcgccgacgc tgttaaaccg agtgatccca ttacgctcaa ggacggccag
      781 tagattgcgc tgaattgcct gcatcccttg cactacggac accggcgcgt cgcccatgtg
      841 atggagggcg cggtcgaaat cgtcaagcac ttcaagcaac gagagaatga gatcacgctt
      901 gccgctttgc gccgcgctgg cgcgatctcg ttctgtacgc cgacgataat tttcaaaatc
      961 ggccagggtg cgcagaagcg tgtcgtgctc gcggcgcaac tcttcgctga gccgggctat
     1021 ttccgcctca gactcggcgg cgcccgactc tgcttgatcg ctcacccggt tttcactttt
     1081 atcaggtgct tccatgagct accccgaggg acattgggac caggctccag ttccaatcaa
     1141 ctttctttga actcagcatc gatcacatca tcgccgcccg aggtgtctgg ccggcctccg
     1201 ggacgctggc cgccggtatt gtcttgactg gaagctgcgg aagagagtcc atagccgact
     1261 tgctgaagat cgctcgtcag ttgccgcagt cgcgccacat cactggaatt ggctttcacc
     1321 aactcgcgta tctgcgagat tagttgctcg gcgcgggctc tctcgttcat cggcacgcgg
     1381 tcacccaact cgctcacttg ccgctccact tgataagcca tcgaatcggc cgaattacgg
     1441 gcttcgatcg cttcgcggcg cttgcggtct tcgtccgagt gcgcctgcgc atccgcgacc
     1501 atgcggtcgc cgccttagca tcgcccccag tttcggacag agcacgatcg tattcacggt
     1561 gccgctcggg atctaacagc gtgtgataag cccggacaag ctcctggaaa aactgtaagc
     1621 gcccagaacc aaagcgttcg ggatgatata gcttcacctg ctgacggaag gcttgacgaa
     1681 ttcattcagc aggttcgccg ggccagaggt cgagcttgaa gtaataatcg ttcatcaacg
     1741 taatcacgca aattgattgc tactagtcat ctatgaaccc ggacagcggc ctttgtcaca
     1801 ggcggccagt tatgaggcgt ccacctacct cgatgggttc gcttgtgatg acccccctag
     1861 gctcctgatc tctcgccctg aaccttgacc gggatctcac gtggttttgc cttttcagct
     1921 cgcggcagct gcagtttcaa cacaccgtga ctcagcgacg ccgagatctt ttcctggtcg
     1981 acctcttcac tgagctcaaa ttgccggaaa aagtttacca gctcgaactc tttgtacgtg
     2041 gcttcgccat ccaccgaatg ttttgggctt gcctgaatag tcagggtgtt ttcttcaagc
     2101 cgcaccttta gatcttccgg cgctaccccg ggcaggtcgg cgagcaacac cagtccgtcc
     2161 gggctgtcat aaatgtccac cggcggc
//
LOCUS       DASUTG010000345         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1389528, whole genome shotgun sequence.
ACCESSION   DASUTG010000345 DASUTG010000000
VERSION     DASUTG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389528"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VF089_07805"
     CDS             <1..712
                     /locus_tag="VF089_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6173904.1"
                     /translation="FNHARHLLTLGAWLMALKAMSLAPTTRLKFEKTPAEIINNKEGE
                     IMKLYYNPKSRAVIAKWMLDECGAKYDVVPLEFEKGEHKSPEFLKINPAGKLPALVDG
                     ESKIFETAAICLYLGDKFTEANLAPGIGAPERGRYLSLMVYSTAQLEPALGDAMLGGQ
                     TSPQRGWADFETVRNVIEGELGNGPYLFGEWFTAADVMIGSMFMWRRMLGGKPDRPKL
                     EAYVDRLIARPAAPKLGG"
     gene            841..1215
                     /locus_tag="VF089_07810"
     CDS             841..1215
                     /locus_tag="VF089_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173905.1"
                     /translation="MSKVSVRYIVNDVDAAIPFYTSMLGFKLEMHPAPGFASLSRGDL
                     QLLLNRPGAGGAGQAMPDSQRPAPGGWNRIQLEVDDLEATVEKLRGARARFRNEIVTG
                     NGGKQILVEDPSGNPVELFQPF"
     gene            1304..1681
                     /locus_tag="VF089_07815"
     CDS             1304..1681
                     /locus_tag="VF089_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173906.1"
                     /translation="MAILLDHTIVPARDKLASAKFFAEIFGLSVKAGDGYFAQVQLND
                     SLTFDFADEPEPQSHHYAFHISEAEFDGVYDRVKSKGLPYGSGPYNHSDGKIYTRRGG
                     RGFYFEDPNGHLLEVMTVPETGS"
     gene            1724..1969
                     /locus_tag="VF089_07820"
     CDS             1724..1969
                     /locus_tag="VF089_07820"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6173907.1"
                     /translation="MATIQKAIAIERSKELVWDAIRDVGAIHKRLVPGFVVDCKLEGD
                     WRTVTFANGMVVRELIVAVDDKTCRYAWSARGASHSP"
     gene            complement(2437..3267)
                     /locus_tag="VF089_07825"
     CDS             complement(2437..3267)
                     /locus_tag="VF089_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6173908.1"
                     /translation="MIIHLIDGTYELFRHFYGLRRFNKGADRPFGAVVGVLNTVLQMI
                     ESGATHVGVATDHVIESFRNQLWPDYKTSDGIEPALLAQFHPLEEALAAMGVAVWPMV
                     ELEADDALASAAKIASVNDDVQKICIWTPDKDLSQCVRGDRIVQVDRRTQKMRNAEGV
                     REKFGVDPVLIPDLLALIGDAADGFPGIAGIGPITAARLLNRHGMIETFPSAVLGDNR
                     ELALLFKNLATLRTDAGLFRDIDELRWHGPTKEFAAYAQQMGEARLLQRCLKAESMIL
                     "
     gene            complement(3352..3621)
                     /locus_tag="VF089_07830"
     CDS             complement(3352..3621)
                     /locus_tag="VF089_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX6173909.1"
                     /translation="MCRNIKRLFNFDPPVSDSEIRAASLQFVRKLSGYNKPSKTNETA
                     FFSAVNEVTMISKHLLASLQSTTAPTTREEQAAKATARAARRFGR"
     gene            complement(3666..4079)
                     /locus_tag="VF089_07835"
     CDS             complement(3666..4079)
                     /locus_tag="VF089_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6173910.1"
                     /translation="MNVALWILQVLLAGLFLFAGGVKLVAPIEEMLKQMPLALPGWFL
                     RLTGVIEVLGAIGLIVPWLTGIRPGLTPLAAAGLVIVMIGAVIFTILAGDIAMALMPL
                     VVGLLCAFVAYGRWRLTPYPGSAQPSRLQPSAKRT"
     gene            complement(4149..4550)
                     /locus_tag="VF089_07840"
     CDS             complement(4149..4550)
                     /locus_tag="VF089_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6173911.1"
                     /translation="MNVLVNLDVDDLDKAVRFYSSVFNLRVGRRLGAVGLEMLGCSSP
                     IYFLERSATAAAETSSQARTYQRHWTPVHLDFVVDELEPVVERAVLAGALLEKPIQVF
                     GWGKLALMADPFGHGFCCVQFIGRGYDEIAE"
     gene            complement(4585..4932)
                     /locus_tag="VF089_07845"
     CDS             complement(4585..4932)
                     /locus_tag="VF089_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6173912.1"
                     /translation="MKYKGSCHCGKIVFEAEGEINVALACNCSICSRKGSLLWFVPRE
                     QLAVFTPEEEIGTYTFNKQVIKHRFCQTCGIHPYGEGTDPNGNKIAAINVRCIEGIDL
                     ASVPVQNFDGRSL"
BASE COUNT         1182 a         1400 c         1361 g         1051 t
ORIGIN      
        1 tttcaatcat gcgcgccatc ttcttaccct cggagcctgg ttgatggcgc tcaaagcgat
       61 gtcgttagca ccgaccacga ggttaaagtt cgaaaaaaca ccagctgaaa taataaataa
      121 taaggaaggt gaaattatga aactgtacta caaccctaag agccgcgccg ttatcgcgaa
      181 atggatgctt gacgagtgcg gcgctaaata tgacgtcgtg cccctcgagt ttgaaaaggg
      241 cgagcacaag agtccggagt tcctcaagat taatccggcg ggaaagttgc cggcgctggt
      301 cgatggtgag tccaagatct ttgagactgc ggcaatctgt ctgtatctcg gcgacaaatt
      361 taccgaagcc aacctggctc ccgggatcgg cgcgccggag cgtggccgat atctctccct
      421 catggtttat tcgaccgcgc aactcgagcc cgccttgggt gacgcaatgc tgggcggcca
      481 gacctcaccg cagcgcggtt gggcggattt cgagaccgtg cgcaatgtta tcgagggcga
      541 actcggcaac ggcccctatt tgttcggaga gtggtttacc gccgctgacg tgatgatcgg
      601 ttccatgttc atgtggaggc gaatgctggg tggcaaaccc gatcgtccga agcttgaagc
      661 ctatgtcgat cggctgatcg cgcgccccgc ggcgccgaag ttaggcggct aacatctcca
      721 aggtctcgag aaagaagcaa cgctcgtaat atgtctggcg accgcgagca ctggacacaa
      781 atcagcgcat gattgtgtct gcgagtcata taaggcacga aacagttagg gagagaaacg
      841 atgagtaaag ttagcgttcg atacatcgtc aacgacgtcg atgcggcaat tcccttctac
      901 acgagcatgc tcggtttcaa gctcgaaatg catcctgccc caggtttcgc gagcctgtcg
      961 cgaggagatt tgcagttgct gcttaatcga cccggtgcgg gaggagccgg ccaagcaatg
     1021 ccggatagcc aacgcccagc accaggtggg tggaaccgaa tacaactcga agtcgatgat
     1081 ctcgaagcta ctgttgagaa actgagagga gcgagagcgc gatttcgcaa tgagatcgtg
     1141 accggcaacg gaggcaaaca gatcctcgtg gaggatccct cggggaatcc ggtcgagctg
     1201 tttcagccct tctgacttcg atcaacgtct gaagattgac ggttataaag aaggttcgtt
     1261 tgataaatga ccggccgatt tatattcggg ataaggagat aacatggcca tcctactcga
     1321 tcacacgatt gtgccggcgc gcgacaagct cgcctcggcc aaattctttg ccgaaatttt
     1381 tggattgagt gtcaaagccg gcgacggcta ctttgcgcaa gtgcaactca acgacagtct
     1441 gacctttgac tttgccgacg agccggaacc tcagagtcat cactacgcct ttcatatcag
     1501 cgaggcggaa ttcgacgggg tttacgaccg agtgaagtcc aaaggcctgc cgtacggcag
     1561 cgggccgtac aaccacagcg atggcaagat ctacactcgt cgcggcgggc gaggatttta
     1621 cttcgaagat ccgaacggtc acctgctcga agtgatgacg gtgccggaga cgggcagttg
     1681 agttgaaccg agcggaacga attagccgct gagtgaagag attatggcga ctatccagaa
     1741 agcaatcgcg atcgagcgga gtaaagaact cgtctgggac gcgattcgag atgtcggcgc
     1801 gatccacaaa cgtttggtcc ctggcttcgt agtagactgt aaattagaag gcgactggcg
     1861 aacggtaaca tttgccaacg gcatggtcgt gcgcgaactc atcgtcgctg tcgatgataa
     1921 aacctgccgc tacgcttggt cggctcgcgg cgcctctcac tcaccataac gcatctgtgc
     1981 aggtattcgc tgacggcgac gacaagtgtc gcatcgtctg gatagcggat ttgatgccca
     2041 atgaggtcgc cgagactatc gcggagatga ttcaacaggg attaagcagg atgaagcgaa
     2101 cgctcgaagg catcccaact gctgactgac ttgctggaca acaccaatac ggtaagctag
     2161 tccggctata gtcctacaag aaagaatcgc agcggcccgc tttaactaac ggcggcccgt
     2221 tgctcgatga ttcgtcggct aaaacgttga tccgccgtag agcatgcgct tatacgttaa
     2281 gatggtcact ggtacattcc gatactcaag cgcgccgtgc tcgcgcccat gcggcgatat
     2341 ggctgcagat ttttcttggg caccggcaag agaaaaccat caacgtagcg catttatcgt
     2401 cctccgtcgt gactgaattt cattaagcca gatcagctat agaatcatgc tttctgcctt
     2461 aagacagcgc tgcagaagac gagcctctcc catctgctga gcgtatgcgg caaattcttt
     2521 ggtgggtcca tgccagcgca gctcgtcgat atcgcggaac aggcccgcgt cggtcctgag
     2581 cgtcgccaag ttcttgaaca gtagcgcaag ctcgcgatta tctcccagta cggcagatgg
     2641 aaaagtttct atcatgccgt gccggttaag cagccgtgca gccgtgatcg gcccgatgcc
     2701 cgcaatgccc ggaaatccat ccgccgcatc accgataaga gcgagcaggt cggggattaa
     2761 aaccggatca actccgaact tctcgcgaac tccttcggca ttgcgcatct tttgagttcg
     2821 acggtccacc tgcactatcc gatcgccgcg aacgcactgc gacaggtctt tgtcgggcgt
     2881 ccagatgcag attttttgca cgtcgtcgtt tacggatgcg atcttggccg ctgaagcaag
     2941 cgcatcatca gcctccaatt cgaccattgg ccacacagcc acacccatag cggcgagtgc
     3001 ttcttcgagc ggatggaact gggcaagcag tgcgggctcg atcccatcgc tcgtcttgta
     3061 atcaggccag agttgattac ggaaggactc gatcacgtga tcggtcgcca cgcccacgtg
     3121 agtcgctcca ctctcgatca tctgcagtac ggtgttaaga acgccgacaa cagcgccgaa
     3181 aggccggtcc gcgcccttgt tgaaccggcg caagccatag aagtgacgga acagctcgta
     3241 ggtaccgtct atgagatgaa tgatcatgct tcatcgaagg tgccgctcca ccggcgccta
     3301 ctcccggcta agtctttgcg gccatgcgct cggccataag ccgctccaaa ttcacctgcc
     3361 gaatctccga gcggcgcggg cagttgcttt agcagcctgt tcctcccgag ttgtcggggc
     3421 cgttgtcgat tgtaatgaag ccagaaggtg ctttgagatc atcgtgacct cattgacggc
     3481 cgagaaaaat gcggtctcat tggtcttcga gggcttgttg tagccgctga gtttccgtac
     3541 gaactgcagc gatgcagccc ggatttccga gtcgctaacc ggcggatcaa aattgaataa
     3601 ccttttgatg tttctacaca tggcgtttga cctcggaacg tgcgcggcgt tctgcttgct
     3661 gagatctagg tgcgctttgc gcttggctga agccgcgacg gctgagccga tccgggatac
     3721 ggcgtcagac gccagcgccc gtaggcgacg aaagcgcaaa gaagccccac aacgagcggc
     3781 atcagcgcca tggcaatatc gccagcgagg attgtgaaga tcacggctcc gatcatgacg
     3841 atcaccagcc cggcagcggc caagggtgtc agacccggcc gaatccccgt cagccaggga
     3901 acaattaggc cgatcgcacc cagcacctcg ataacaccgg tgagacgcaa gaaccagcct
     3961 ggcaacgcta gcggcatttg cttcagcatc tcctcgatcg gtgcgaccag cttcacgcca
     4021 ccagcgaaca gaaacaggcc ggcaagcaac acttgaagga tccacagtgc aacattcatg
     4081 attgatctcc ttcatctcga ttgtaacagc gcatagctcg ggacgcactg gcagggcatg
     4141 cccgaccgtt actctgcgat ttcgtcatac ccgcggccga taaactgcac acaacaaaat
     4201 ccatggccga acggatcagc cataagggcg agcttgcccc acccaaatac ctgaatgggc
     4261 ttctccagca acgctcccgc taacacagct cgctcaacca ccggttcgag ttcatctacg
     4321 acaaagtcga gatgcaccgg cgtccaatgc cgctgataag ttcgagcctg cgaacttgtc
     4381 tctgcagcgg cagtcgctga tctctcaagg aaatatatcg gagacgaaca gccaagcatt
     4441 tccaaaccga cggcgccaag gcggcggccg actcgtaaat tgaatacaga actgtaaaag
     4501 cgaaccgcct tatcgagatc atcgacatcc aaattcacca ggacattgat catgccgtcc
     4561 ctcggccacg tcccgcggga cccttcatag agaccggccg tcgaagttct gaacgggaac
     4621 gcttgccagg tcaatccctt cgatgcaccg aacattgatt gcagcgatct tgttgccgtt
     4681 gggatcggtg ccttcgccgt atggatggat accgcatgtc tgacaaaagc ggtgcttgat
     4741 cacctgcttg ttgaacgtgt aggttccgat ctcctcttcc ggcgtgaata cagccaactg
     4801 ttcgcggggc acaaaccaca agagcgatcc cttgcgcgaa cagatagaac aattgcatgc
     4861 cagtgccacg ttaatttcac cttcagcttc gaacacaatc ttcccgcagt gacagctgcc
     4921 cttatacttc acatgagctc tccttattcg gccttcgact tcaagtttat ccgtgcacgt
     4981 tcctggaata agcc
//
LOCUS       DASUTG010000346        10849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_351429, whole genome shotgun sequence.
ACCESSION   DASUTG010000346 DASUTG010000000
VERSION     DASUTG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351429"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VF089_07850"
     CDS             <1..1024
                     /locus_tag="VF089_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173913.1"
                     /translation="GERNGGVIETILGRNKIEHFQFYHELAKRTQRPVLWQSLQHRWA
                     EPNLWREQLDAVEPIFREGYQAYGLSHTVPLVRHFELKNCQVFDEFPTWKNLMFLPVE
                     VRKQAFADNETRQKLRADLANPAPTNFHRNWNIVRIEKVVKPENQKYNGKTVAELGAM
                     RNQDPLDAFLDLALEEDLGTVFWNANNGGDINAMGEILRSPYVLVGTSDAGAHVQFGA
                     DFGYCTILLGMWVRERQVMSLEQAVHKLTFHVASIFGLQDRGLLRPGYAADLAIFDPK
                     TVNSHEPEWAYDYPAGSKRLIQRSVGMHYTIVNGTVIHEDSRMSGDLPGQVLRGPLYR
                     EQKVAA"
     gene            1029..1352
                     /locus_tag="VF089_07855"
     CDS             1029..1352
                     /locus_tag="VF089_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6173914.1"
                     /translation="MGVSYIPVARIGELAAGGMKEVDLGGRQVLLTFVEGKYFAFSRQ
                     CPHEDADLQSAGQLLNGTKLRCNNHGYCFDLKTGECMLPQGGPPLTVLPAEEKGEDVC
                     IRLEW"
     gene            1544..2431
                     /locus_tag="VF089_07860"
     CDS             1544..2431
                     /locus_tag="VF089_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX6173915.1"
                     /translation="MTLHKKLRLLALLGLGLSVALAGQLSAQEKSFLWKVDSDQNSLY
                     ILGSIHLLNKEAYPLKQSIQNAFEQTNKLVLEIDLRSANSDRVQQLMLEKGASTDGTL
                     LHQKISKETYESVAMRAKELDIDIRLLNSFKPWIVATTMAAVKLQKLGFDSKLGIDRY
                     FADRAIRSNKPIGGLETAEFQLGLLDQFSAKEQELLLRQSMNEMDHLERNVAEIVRAW
                     KSGDAGSLEKHLLAGMRDYPEIHQKVIEDRNRRWLPQIEALLSQGENALIIVGAAHLV
                     GTNGVISLLKSRGYRVEQQ"
     gene            2431..2970
                     /locus_tag="VF089_07865"
     CDS             2431..2970
                     /locus_tag="VF089_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6173916.1"
                     /translation="MEDAKHSNEIKVADWMTETVLSVEIYDSISVARQLMAKHRVNQL
                     PVLDHERLMGIVTDRDIRDAYPTSMLIGRLKEIDKFAESYTVEEVMTHDVFTVQPETP
                     LATAVALLRHHRIGSLPVVKGKQLVGIITRSDILDFVLSGAGINVGAGRKKARKKLPA
                     SKKGKRSKTTGDRRSSKIF"
     gene            3230..3721
                     /locus_tag="VF089_07870"
     CDS             3230..3721
                     /locus_tag="VF089_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6173917.1"
                     /translation="MASFDVVNRIDLQEVDNAVNITKKTLQSRYDFRESKTEIVLDKK
                     DKKINVITEDEMKMRAVQETLVENLVKRKVDTRCLEFKTSEMAAQGMIQKTIAIKEGI
                     DSDTARAIVKMIKDQKMKVQAAIQEKQVRVTAKKIDDLQAVIKLLRSSNLPIPLQFIN
                     MQS"
     gene            3748..4152
                     /gene="msrB"
                     /locus_tag="VF089_07875"
     CDS             3748..4152
                     /gene="msrB"
                     /locus_tag="VF089_07875"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6173918.1"
                     /translation="MSEAKEKIEKTDEEWRKELTPEQYEVCRGKGTERAFTGKYWDCH
                     DQGVYHCACCGNELFSSETKFDSGTGWPSFYQPIAEEKVETETDNSFLMRRTEVLCSR
                     CNAHLGHLFDDGPAPTHLRYCINSASLKLEKK"
     gene            4167..4622
                     /locus_tag="VF089_07880"
     CDS             4167..4622
                     /locus_tag="VF089_07880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6173919.1"
                     /translation="MDGLAFQDQGTVRYCHGCGPDNEQGLRIKSYWDGDEAIANWRAQ
                     PHHCGGTKQNLNGGIIASLIDCHSLNLAIAHAYKQEQRPIGSKPRIGYVTANLNVSYL
                     KPTPILEPIQLRARIIKIDGRKAWVSCALTAAGELRATGEVLGVRVNWE"
     gene            4739..5683
                     /locus_tag="VF089_07885"
     CDS             4739..5683
                     /locus_tag="VF089_07885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173920.1"
                     /translation="MEYTILGRTGLKVSVAGLGCGGPSRLGLRNSADTEAGAVSLVRQ
                     AIDLGINFLDTAQSYGTEPVVGKAIAGVPRDQLIISTKKTLPPVDHSNPAEDVSDGLE
                     QSLRLIGTDYIDIYHLHGVEPKDYGFAKEKLMPAMRRLRDQGKIRFIGVTEGFVTDTS
                     HQMLQQSLREDMWDVVMVGFNLLNPSARRTVFPLAAAKNVGVLDMFAVRRALSQPDRL
                     TSLFRELIQKRVISASAVDANDPLGILLEEGVAATVPEAAYRFCRHEPGVHLVLTGTG
                     NPEHLKANVKAILKPPLPQPALQRLEELFGRLDSLTGN"
     gene            complement(6247..7362)
                     /locus_tag="VF089_07890"
     CDS             complement(6247..7362)
                     /locus_tag="VF089_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6173921.1"
                     /translation="MTTIPGAATLAGTDRYRERFGGRIHRDHFRQSQGLWMSSIGIGT
                     YLGDYDAETDRQYSQAISRAVQCGCNVIDSAINYRLQRSERAVGAALQDLAAKGFERD
                     EIIVSTKGGFIPYDSAPPADLRRYFEETFFEPGIATAADIVSNSHCMTPAYLLNQLDC
                     SLKNLSLDCLDVYYVHNPETQLGKIPREEFNSRLLKAFEAMEQAVSAGKIRMYGTATW
                     NGYRNDPQARDYLSLAEIIGIATKAGGKDHHFRVIQVPFNLGMNEALSLSNQIVEGKT
                     LTLLEAAQAFDITVMCSASILQGQLAANLPSMIGAALSGLETDSQRSIQFVRSTPGVT
                     TALVGMKQLKHVEENMRTARVPPAPWEQYSKLFESNP"
     gene            complement(7657..8424)
                     /gene="gloB"
                     /locus_tag="VF089_07895"
     CDS             complement(7657..8424)
                     /gene="gloB"
                     /locus_tag="VF089_07895"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX6173922.1"
                     /translation="MKIIQVPLLRDNYAYLLICEKSKQAAIIDPSEAEPVLRRVEQEK
                     INLTAILNTHHHRDHTGGNEGILAQHKVDVYGHKSDSARIFGLTRGVEEGDEIKIGDL
                     AGKVLFVPGHTSGHITYLFGSNLFSGDTLFTAGCGRLFEGTPEQMHGSLKKLAALPDE
                     TKVYCGHEYTESNLRFAVTLEPKNHKLVSRFERVQGLRTRGASTVPATLEEEKQTNPF
                     LRWDSKELQATLKSQDPDLRLDPVSVFAKVRQLKDSF"
     gene            8898..9254
                     /locus_tag="VF089_07900"
     CDS             8898..9254
                     /locus_tag="VF089_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6173923.1"
                     /translation="MAISAKGFSLIELLVVVAIIGLLTAIAIPQFLSYRAHAVDSQMK
                     SDLKNAALAMDSYFAENKVYPTSVAAIVGVGFNQTEGVALTIAVPTPSSFTITASKPS
                     GTQPSFTFSSVTGNIN"
     gene            complement(9261..10226)
                     /gene="corA"
                     /locus_tag="VF089_07905"
     CDS             complement(9261..10226)
                     /gene="corA"
                     /locus_tag="VF089_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX6173924.1"
                     /translation="MLINCAAYENGHKLADISVEEIPAYISRPACFVWIALKDPEPSE
                     LETMQQVFGLHELAVEDAQHGHQRPKIEEYGESLFVVLHMIEFAGDELVVGETEIFVG
                     RNYVLSVRRQAQRGFVEVRQRCEREPELMKHGTGYVLYALMDAVVDRYFPILDALETN
                     LERIEEQIFANQSSRANIEDLYELKRKLTVLKHATEPLIEAITRLFGGRVPPLCASLQ
                     DYFRDVYDHLIRLNQSIDSLRDMVVTAISVNLSLISIQENEITKRLGSYAALITVPTL
                     IAGIYGMNFHNMPELDSPFGYPLALISMVVIDAYLFIRFRKAGWL"
     gene            complement(10390..>10849)
                     /locus_tag="VF089_07910"
     CDS             complement(10390..>10849)
                     /locus_tag="VF089_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6173925.1"
                     /translation="RIAVVDMPEEEWDRVMRINLKGVFLCSQAAAKHMIPKKKGRILS
                     IASGRGVAGQARAAHYAASKAGVIAFTKSLAMELAPYDITVNAVCPGATDTPMSRIGF
                     TSEQFAKRQEVPPLLDGLTKKDEIVGLVRYLLSDATRFVTGQTFFLRTPR"
BASE COUNT         2570 a         2829 c         3010 g         2440 t
ORIGIN      
        1 gggcgagcgc aacggcggtg ttatcgagac gattctgggc cgcaacaaaa ttgaacattt
       61 tcaattttac catgagctgg cgaaacggac gcaacgaccg gttctctggc agagcctaca
      121 acaccgctgg gcagagccca atctctggcg cgaacagctc gatgcggttg agccgatttt
      181 ccgcgaaggc tatcaggcct atggcttatc ccataccgtg ccgctcgtgc gccacttcga
      241 gctcaagaac tgccaggtct tcgatgaatt cccgacctgg aaaaatctca tgttccttcc
      301 cgtagaggtg cgcaagcagg ctttcgccga taacgaaacc aggcagaagc tgcgagccga
      361 tctggccaat ccggcgccga ccaactttca ccgaaactgg aacatcgtcc ggatcgagaa
      421 agtggtgaag cccgagaacc agaagtacaa cggcaagacg gtggctgagc tgggagcgat
      481 gcgcaatcaa gatccgctcg acgccttcct ggatctcgcg ctcgaggaag atctcggcac
      541 ggttttctgg aacgccaata acggtggcga catcaacgcc atgggcgaga tcctgcgcag
      601 cccgtatgta ttagtcggaa cttccgatgc aggggctcat gtgcagtttg gcgccgattt
      661 tggctattgc accatccttc tgggtatgtg ggtgcgcgag cgtcaggtaa tgagcctaga
      721 acaggccgtt cataaactga ctttccatgt cgcatccatc ttcggcttgc aggatcgcgg
      781 tttgttacgg ccgggttatg ccgcagacct ggcgatcttc gatcctaaga ccgtcaactc
      841 tcatgaaccg gaatgggcct atgactatcc ggccggaagc aagcgtttaa ttcagcgttc
      901 ggttggaatg cactacacca tcgttaatgg cacggtaatt catgaagaca gccgcatgag
      961 cggtgatcta ccaggccagg tgctgcgcgg gccgctctac cgagagcaaa aggtggcggc
     1021 ataatcggat gggagtgagc tatataccgg ttgcccggat tggcgagttg gcagcgggcg
     1081 gcatgaaaga agtcgacctc ggcggacgtc aggtgctgtt gacgttcgtc gaggggaaat
     1141 attttgcgtt ctcgagacaa tgtcctcatg aagacgccga cttgcaatcc gccggacaac
     1201 tgctgaacgg aaccaagttg cggtgcaaca atcatggcta ttgttttgac ctgaaaacgg
     1261 gtgagtgcat gctgcctcaa ggcggtccgc cgctgacggt gcttccggcg gaggaaaaag
     1321 gcgaagatgt gtgcattcgc ctcgagtggt aagtaatccc atctttttgc ctcgggattg
     1381 cttctgaggc aatccgcggg ggccatcatt cacgttccaa tactgccgat ccgattcaca
     1441 acccatcgca gctctggtca actttcgcga gaaaaggtaa gtttctacat ggatgtgttt
     1501 ccaggcctga tcgttggacc aggctcgtga accgcaaaac cttatgactc ttcacaaaaa
     1561 attgcgtttg ctggctctgc tcgggcttgg attatctgtt gctctggccg gccagctaag
     1621 cgcacaggaa aaaagctttc tctggaaagt ggattcagac cagaacagtc tctatatcct
     1681 cggctcgatt catttgctga ataaggaggc ctacccactc aagcagagca tccagaacgc
     1741 cttcgagcag acgaataaac tcgtcctgga gatcgatctg cgcagcgcaa actctgatag
     1801 ggttcaacaa ttaatgcttg aaaaaggtgc cagcaccgac ggcacgctgt tgcatcagaa
     1861 aatctccaaa gagacctatg agagtgttgc gatgagggcc aaagagctgg acatcgatat
     1921 tcggctgttg aattctttta agccctggat cgtagcgact acgatggcgg cggtgaaact
     1981 gcaaaaactg gggtttgatt caaaattggg catcgatcga tactttgccg atcgcgccat
     2041 tcgatccaac aagcccattg gcggcttaga aacggctgaa tttcagctcg gcttgcttga
     2101 tcaattttct gcaaaggagc aagagctctt gctgcgccag agtatgaacg agatggatca
     2161 cctggaacga aatgtggcgg aaatcgtgcg agcctggaaa agtggcgatg ccggatcgtt
     2221 ggaaaaacat ctcctcgcgg gcatgcgcga ttatccggag atccaccaaa aggttatcga
     2281 ggatcgaaac cggcgctggc tgccgcagat cgaggccctg ttgtcacaag gcgagaacgc
     2341 gttgatcatt gtcggtgcgg ctcatctggt cggcaccaat ggcgtcatct cgttgttgaa
     2401 aagccgaggc tatagagtgg agcaacaata atggaagatg caaagcactc caacgagatc
     2461 aaggtggcag actggatgac cgagacggtc ttgtcggttg agatctatga tagtatttcc
     2521 gttgcgcggc agctcatggc caagcaccgc gtaaaccagt tgccggtgct cgatcatgaa
     2581 cgcttgatgg ggattgtcac ggatcgagac atccgggatg cgtatccgac gagcatgttg
     2641 atcggacgcc tcaaagagat cgacaagttc gccgagtcct ataccgtcga agaggtgatg
     2701 acgcatgatg ttttcacggt gcaacccgaa acgccgctag ctaccgcagt ggcgttgttg
     2761 cgccatcacc gaatcggttc gctgccggtg gtcaaaggaa agcagctcgt gggaataatc
     2821 acccgcagcg atattttgga ttttgttttg agcggggccg ggatcaacgt gggagccggg
     2881 cggaaaaagg cgcgcaagaa attgccagcg tcaaaaaagg gcaagcggtc caaaactacc
     2941 ggcgatcgca ggtcaagcaa aattttctga cagatcgttg aaaaccttgc aagctaccca
     3001 gaaacagccg cgaggcggcg atcgagccaa tgacccctag cagggtgctg aaaaaagcct
     3061 cttgcaggct gctcaaaaag atccaggtgc gaggcgcgcg aaaaatcgac gagcggaggc
     3121 gtacttacag tacgttgcag cgaggcgatt gagcggcaac gaagcatatg ggtctttttc
     3181 accagcctgc tagagtcttc agtaactccg ctatagcagg agaaaaatca tggcgtcttt
     3241 cgatgtggtg aatcgaatcg acctgcagga agtcgataac gcggtgaata tcaccaagaa
     3301 gacacttcaa tctcgctacg attttcgcga atcgaaaacc gagatcgtgc tcgacaagaa
     3361 agacaagaag atcaatgtca tcaccgaaga tgaaatgaag atgcgcgcgg tgcaggagac
     3421 tttggtagaa aacctggtca agcggaaagt cgacactcga tgtttggaat tcaagaccag
     3481 cgaaatggcg gcacagggga tgatccagaa gaccattgcc atcaaagaag ggatcgattc
     3541 cgatactgcc cgcgcgattg tcaagatgat caaagatcag aagatgaagg ttcaagcggc
     3601 catccaggaa aagcaggtaa gggtgacggc caaaaaaatc gacgatctgc aggctgtcat
     3661 caaactgcta aggtcgtcga atctaccgat tcccctgcag ttcatcaata tgcagagcta
     3721 gcgatcaggg tggttcagga ggaaaccatg agtgaagcaa aggaaaagat tgaaaaaacc
     3781 gatgaagagt ggcgcaaaga gctaacgcca gagcagtacg aggtgtgccg cggtaagggc
     3841 acggagcggg ctttcaccgg gaaatactgg gactgtcacg accagggagt ttatcattgt
     3901 gcctgttgcg gcaacgagct attcagctcc gagaccaaat tcgattccgg caccgggtgg
     3961 ccgagttttt accagccgat tgccgaggaa aaggtcgaga ccgaaaccga caacagtttt
     4021 ctaatgcgcc gcacggaagt gctttgcagc cgatgcaacg ctcatctggg ccatctcttc
     4081 gacgacggac ctgcgccgac tcacttgcgc tactgcatca attcggcctc attgaagctg
     4141 gaaaagaagt agacctctgc cgttcgatgg atgggctagc attccaagat caggggacgg
     4201 tgcgctactg ccacggttgc ggtccggaca acgaacaagg cttgcggatc aaaagttatt
     4261 gggacggcga tgaagcgata gcaaactggc gggctcagcc gcaccattgt ggcggcacga
     4321 aacaaaatct caatggcggc atcattgctt cgctcatcga ctgtcacagc ttgaatctgg
     4381 ccattgcgca cgcgtacaag caggagcaac gtccgatcgg cagtaagccc cgcatcggct
     4441 atgtcacggc aaatttgaac gtgtcctatt taaagcccac gccgattcta gagccgatcc
     4501 aattgcgcgc gcggatcatc aaaatagacg gacggaaggc ttgggtcagt tgcgcattga
     4561 ccgccgccgg cgagcttcga gcgacaggag aggtgctcgg agttcgggtg aattgggagt
     4621 aatccgatca aaatgttcaa gccgttcaaa tcgttcaagc cgatgaaatc cgcgccgctc
     4681 ttaacgtttt gaactatttg aacgtgttga acggcttgaa cagacgtttc tgggattgat
     4741 ggaatacact atcttagggc ggaccggttt gaaggtaagc gttgcgggtc tgggttgcgg
     4801 cggaccaagc cggctcgggc tgcgcaacag cgccgacacc gaagccggcg ccgtttcgct
     4861 ggttcgacag gcaatcgatc taggtatcaa ttttctcgat acggctcagt cgtatggaac
     4921 cgagcccgtc gtcggcaaag ccattgccgg agtaccgcgg gaccaattga ttatctcgac
     4981 caaaaagacc ttaccgccgg tcgatcacag caatcccgcc gaggatgtca gcgatggact
     5041 ggagcagagc ctgagattaa tcggcaccga ctatatcgat atttatcatc ttcatggggt
     5101 cgagccgaaa gattacgggt ttgccaaaga gaaattgatg ccggccatgc ggcgccttcg
     5161 tgatcaaggc aagattcgtt tcatcggtgt gaccgaaggg ttcgtgaccg atacgtcgca
     5221 tcagatgctg caacaaagcc tgagagaaga tatgtgggac gtggtgatgg tcggctttaa
     5281 tcttctcaat ccctcggcgc gcagaaccgt ctttccattg gctgccgcaa agaacgtcgg
     5341 tgttttggac atgttcgccg tccgcagggc cctcagccag ccggatcgtt taacaagctt
     5401 gttccgggag ttgattcaaa agagagtgat ttccgccagc gccgtcgatg cgaatgatcc
     5461 gcttggaatt cttctcgagg aaggggtggc tgcgacagtc ccggaagcgg cttatcgttt
     5521 ctgccggcat gaaccaggcg tccaccttgt cctcaccggg accggtaatc ccgaacatct
     5581 taaagccaat gtcaaagcca ttctgaagcc gcccttgccc caaccggctt tgcagcgcct
     5641 ggaggaactc ttcggaaggt tagattcgct gacgggaaac taactggcta ctaaccccgg
     5701 attccgcagt tcaaagttat acagcgatcg agcgccaagc cctccggagc gaagacaatg
     5761 ggcccgtggc ttggagcggc ctgcaagctc ctgtgctgag tggacgcctc gtaaaattgg
     5821 cccccccgaa ccacacagcg cagacgtatt gacactgttc gctctttgtc tacgctctcc
     5881 tccaggctcg cccctcgatc agtatacggc tgaccattct ctactggcgg aatccggcgt
     5941 gttttgaaga ctgttagaat cgtgctcaga gtgcgtgtgc gttcggaatt ttcgatcacg
     6001 tttttacgga cacgatcacg aggatcccgt ctcggcttca gaatcttttc aactcagcct
     6061 gccgtccggt accggctgat cagaaatcta gcaggctgct gaaaaagact cataggctgc
     6121 gttccgctca atcgcctcgc tgcaacgtac tgtaagtacg cctccgctcg ttgctttttc
     6181 gcgcgcctag cctctggatc ttttgagcag cctgcaacca gtgtatttca cagcttgcta
     6241 gtgattttac gggttcgatt caaatagctt ggaatactgc tcccatggcg cgggtgggac
     6301 tcgggcagta cgcatgttct cttcgacatg cttcagctgt ttcatgccta ccagcgcggt
     6361 ggtgactccc ggggtcgaac ggacgaactg aatcgatctt tggctgtcgg tttcaagccc
     6421 gcttaatgcg gcaccgatca tcgacggaag attggcagcg agctggccct gtagaatgga
     6481 ggcgctgcac ataaccgtaa tgtcgaaggc ttgcgccgct tccaaaagcg taagggtttt
     6541 gccttctact atttggtttg acagggacaa agcttcattc atgccaagat tgaacggcac
     6601 ttgtatcact ctaaagtgat gatctttgcc gccggctttt gtcgctatac cgatgatctc
     6661 ggccagcgac aggtaatccc tggcctgcgg atcattgcgg tatccgttcc atgtcgcggt
     6721 gccgtacatg cgaattttcc ccgccgagac cgcctgctcc atcgcctcga aagccttcag
     6781 caaccgactg ttgaattcct cgcggggtat tttgcctaac tgggtctccg gattatgcac
     6841 gtaatagaca tcgaggcaat caagagacag gtttttcaaa ctgcagtcga gctgattcaa
     6901 tagataagcc ggggtcatgc aatgagagtt gctgacgata tcggccgcgg tcgcgatgcc
     6961 cggctcaaaa aaggtctctt cgaaatagcg ccgcaagtcg gccggcggcg cgctgtcgta
     7021 aggaatgaac cctcctttgg tcgagacgat gatttcatcc cgttcaaatc ctttggcagc
     7081 aaggtcctgc aaggctgcgc ccaccgcgcg ctcgcttctt tgcaggcgat aatttattgc
     7141 gctgtcgatc acattacagc cacactgaac cgcgcggctg atagcttggg agtactgacg
     7201 gtcggtctcg gcgtcgtaat caccgagata agtgccgatc ccgatggagg acatccacaa
     7261 gccttgagac tgccgaaagt gatcgcggtg aattctgccg ccaaaacgct cgcggtaacg
     7321 atccgtgccc gcaagtgtcg ctgcgccggg gattgtcgtc atcgtgcacc ttacctttgc
     7381 cgctcaagaa tagatcgtct gaatcaaagc ttctgctgcc tggtgaaaat caaaaccagc
     7441 gcgacggtaa acatttgcaa gctgggaagc gacgccgcct ggaagaagag cagtgtaaca
     7501 gagacgggtt cctcgtggaa agttcattgg caagcagttg cgaccagcaa cactgcttat
     7561 ttgagtggag gcgcaggggc agctgtctta acaggttcag ggtggcgcga tgcctgagcg
     7621 ctccagaatg cgcagccttg cttcgagcca agccgtttaa aacgaatctt tcagctggcg
     7681 cactttcgca aatacggaaa caggatctaa gcggagatca ggatcctggg atttgagggt
     7741 tgcttgaagc tctttactat cccagcgcaa gaacgggttt gtttgctttt cttcttccag
     7801 ggtggcggga acggtggaag cgcctcgtgt tcttaatcct tgcacccgct caaatcgaga
     7861 gaccagcttg tggttcttgg gttccagtgt cacagcaaaa cggagattgc tttcggtata
     7921 ctcgtgaccg caataaactt tcgtctcatc cggaagcgcc gccagttttt tgagcgatcc
     7981 atgcatctgc tcgggcgtgc cctcgaacag gcggccgcag ccggcggtga atagggtgtc
     8041 gccggaaaag aggttgctgc cgaagaggta agtgatatga ccgctggtgt gccccggaac
     8101 gaaaagaact ttgccggcca aatcaccgat cttgatctca tcgccttctt cgacgcctcg
     8161 ggttaagccg aagattctcg cgctatcgct cttgtgaccg taaacatcga ccttatgctg
     8221 cgcaagaatg ccttcgttgc cgccggtatg atcgcgatga tgatgcgtgt tcagaatcgc
     8281 cgtaagattg atcttttcct gttctacgcg ccttagcacc ggctcggctt cagaagggtc
     8341 gatgatcgcc gcctgtttgc tcttctcgca gatcagtaga taagcgtaat tatcccgcag
     8401 tagcggaacc tgtatgatct tcatgtgtta ggaatgcgcc tttcgaatcg gagcgagccc
     8461 cgtccctgtt gtgatagata gctatcacag atcatcattt atctaaagcg aaagtccagt
     8521 agaaactgag tcgcgcgtcg cttacgggcg gagtagatga agttcgtacc aagctccggg
     8581 agcttcgcgt atgcttaaag atctgctgct tcctgtccgg acccggtcca tggatctact
     8641 cgaatgttga aagataaaac ctgagaattt taatcaggct ggttcttctt taatgaaagc
     8701 agtctttcga cctcacggag aatcgcattt cttcaaagtt gaagatgtcg aaatagcgcc
     8761 caccacgctc gcggtttggc cgtagaccct tagtcaaaaa attggtgttc cgggtacgtt
     8821 ctgtgttaaa ctgggcgcgt tttattcggc gagccgcgat ccgactgctt atgcacctcc
     8881 gagtttcgca acctaagatg gctatcagcg ccaaaggctt cagcctgatc gagctgctgg
     8941 tggtcgttgc catcattggc ctgttgacgg ccattgccat cccacagttt ttatcatatc
     9001 gggcgcacgc cgtggatagc caaatgaaaa gcgatctcaa gaatgccgct ctggcaatgg
     9061 actcctactt tgccgaaaac aaagtatacc ccacttctgt tgccgctatc gtaggcgtgg
     9121 gctttaatca aacagagggc gtagccctga cgattgccgt tcccacgccc tcttcattta
     9181 caatcacggc cagcaagcct agcggcaccc agcctagttt caccttcagt agtgtcaccg
     9241 gcaatatcaa ctaacgctgc ttacaaccaa ccggctttgc ggaaacggat gaaaaggtaa
     9301 gcgtcgatga ccaccatgct gatgagcgcc aggggataac cgaacgggga atccagttca
     9361 ggcatgttgt ggaaattcat cccgtagatt ccggctatca aggtcggtac ggtaatcagc
     9421 gccgcatagg agcccaagcg tttggtgatt tcattttcct gaattgagat gagcgacaga
     9481 ttgactgaaa tcgccgtgac caccatatcg cgcaggctat cgatggactg attcaagcgg
     9541 atcagatgat cgtaaacatc gcgaaaataa tcctgcaagc ttgcacagag tggcggcacc
     9601 cggccaccga agagccgggt gattgcttca atcagcggct cggtggcatg tttcaaaacg
     9661 gtgagtttgc gcttcagctc gtagaggtcc tcgatatttg cgcggctact ttgattagcg
     9721 aatatttgct cttcgatgcg ctcaagattg gtctccaacg catccaggat ggggaaatag
     9781 cggtctacca ccgcgtccat cagcgcatag agcacgtatc cggtgccgtg tttcattagc
     9841 tccggctcgc gctcgcagcg ctggcgcacc tcgacgaacc cgcgctgcgc ctggcggcga
     9901 accgataaga catagttccg gccgacgaag atctctgttt cgccgacgac gagctcatcg
     9961 ccggcaaact caatcatgtg cagaacgaca aaaagcgatt cgccgtactc ctcaatttta
    10021 ggccgctgat ggccatgttg cgcatcttcg acggcaagct cgtgcaggcc gaagacctgc
    10081 tgcatggttt cgagctcgct cggctccgga tctttcaggg caatccacac gaaacaggcc
    10141 ggccggctga tataggcagg tatttcctcg acagaaatgt cggcaagctt atgaccgttc
    10201 tcgtaggcgg cacagttgat gagcatagtg tctgagctca gtcgttcgac aggagttctg
    10261 atagttccgc ggttcgacgt tagaaatatt ttccggggct caacctatcg agctggttcg
    10321 gacgcccaaa ggtcagtcac aggttcatgg cagctaacaa ggttgttgca ggttccctgc
    10381 cggcggtttt cacctgggag tgcgcaaaaa aaacgtttgt cctgtgacga acctggtcgc
    10441 atcactcagc aaatacctaa ccagaccgac gatctcgtcc ttttttgtga gcccgtccag
    10501 gagcggcgga acttcttggc gctttgcgaa ctgctccgat gtgaaaccga tcctcgacat
    10561 gggcgtgtcg gttgctcccg gacagaccgc gttgacggtg atgtcgtatg gcgccaattc
    10621 catggccaat gatttggtaa aagcgatcac accggctttg gacgcagcat aatgagcggc
    10681 tcgagcctgt ccggcgacgc cgcgccccga agcgatgctc aagatgcgcc cctttttctt
    10741 ggggatcatg tgtttggcgg ccgcttgaga acaaagaaag actcctttga gattgattct
    10801 catgacacgg tcccattctt cctcgggcat gtcgacgacc gcaatgcga
//
LOCUS       DASUTG010000347         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_795829, whole genome shotgun sequence.
ACCESSION   DASUTG010000347 DASUTG010000000
VERSION     DASUTG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795829"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1780)
                     /gene="amt"
                     /locus_tag="VF089_07915"
     CDS             complement(176..1780)
                     /gene="amt"
                     /locus_tag="VF089_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX6173926.1"
                     /translation="MKKLRKLLAFASLSFVVGIFLSSSLSAQENPAPAEPTKIEAPAV
                     SSGAGVEVKKEDPGAEPVQFTNGLLEQIGGAKVAIDTMWVLIAAMLVFFMNLGFASVE
                     AGLCRAKNAVTILAKNFVVFAAASIAFLGLGWGLMFGDGNPFFGTQGLWFVGGADNSP
                     AMGDAYNGVYSSINWTGVPLWSKFFFQLVFAGTAATIVSGAVAERIKFGSFYIFSFLM
                     VGLIYPIVGHWIWGGGWLAKLGMFDFAGSTVVHSVGGWAALSGAMILGPRIGKYSKDG
                     KTLPLPGHSIALATIGVFVLWFGWFGFNPGSTMAADAGAIGRIAVATNTAAAAAAISA
                     SIAAWVLLGKPDLTMILNGALAGLVAITAPCAFVSIPSSLIIGLVAGVLVVLSVLFFD
                     KIKIDDPVGATSVHLVCGIFGTICVGLFAQAEFTPNTTGDGLLFGGGTGLLMAQLAGV
                     VGVGVFVFPLSLIVWKIIDATLGVRVSPEEEVEGLDISEHGNVAYPDFVTVTSRASTM
                     VAGTTGTLGDAPAGARAPALSKGTVS"
     gene            complement(1814..2152)
                     /locus_tag="VF089_07920"
     CDS             complement(1814..2152)
                     /locus_tag="VF089_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6173927.1"
                     /translation="MKLVEAIVKPFKLEEIKETLTKAGIQGMTVEEVKGFGRQKGHTE
                     LYRGAEYTVDFLPKVRLEVLVADDKAAKVVELITESARTGKIGDGKIFVTPVDEVIRI
                     RTGERGEDAI"
BASE COUNT          591 a          713 c          570 g          521 t
ORIGIN      
        1 gttgaaaaag caggatctct tccattggat ctagctcgat ggattggatg ccgcatagca
       61 gagtacggcc gagttctaag tattcgctgg tttagcgaat accattttca tagtaccttg
      121 ccggcccatt aagcctggtc cgaaaaaaca gacctgaggc gccatgtctc cggtctcacg
      181 aaaccgtccc cttggaaagc gcaggcgcgc gagcgcccgc cggagcgtcg cctaaggtgc
      241 cggttgtgcc cgcaaccatt gtgctagctc tcgatgtaac ggttacaaag tccgggtacg
      301 cgacgttacc gtgttcgctg atatctagac cttcaacttc ctcttccggc gacactcgta
      361 cgccaagagt cgcgtcgatg atcttccaga cgatcagcga gagtggaaaa acgaaaactc
      421 ctacgcccac cacgcccgca agctgcgcca taaggagccc ggtacctcca ccgaagagca
      481 aaccatcacc tgtggtgttg ggcgtgaact cggcctgggc aaataagcca acgcagatcg
      541 tgccgaaaat gccgcaaacc aaatgcaccg acgtcgctcc aaccggatcg tcgattttga
      601 ttttgtcgaa gaatagaact gataatacga ccaacactcc ggcaaccaag ccgattatta
      661 gtgagcttgg gatactgaca aaggcgcagg gcgcggtaat cgccacgagt cccgccaacg
      721 cgccgttgag gatcattgtt agatccggtt tacctagaag cacccacgct gcgatggatg
      781 ccgaaatagc cgctgcggct gccgccgtat tggttgccac cgcgattcgg ccaatggctc
      841 cggcatctgc cgccatggtg ctgccgggat tgaacccgaa ccagccaaac caaagaacga
      901 atacgccaat ggttgccaat gcgatactgt ggcccggcag tggcagcgtc tttccatctt
      961 tactgtattt accaatgcgc ggacccagaa tcatcgctcc cgaaagcgcc gcccatccgc
     1021 ccacggagtg aaccactgtc gatccggcga agtcgaacat acctaatttc gccagccagc
     1081 ctccgcccca tatccagtgg ccaacgatcg gatatataag acccaccatc aaaaaactga
     1141 aaatgtagaa agatccaaat ttgattctct ccgccacggc gccggaaaca atcgtagctg
     1201 cagtgccggc aaacacaagc tgaaagaaga acttgctcca caatgggacg ccggtccagt
     1261 tgattgatga atagacaccg ttgtaagcat cccccattgc cggactgttg tcggcgcctc
     1321 cgacaaacca taagccctga gtaccaaaaa acgggttgcc gtcgccgaac atgagtcccc
     1381 aacctaaacc taaaaacgct atcgacgccg cggcgaacac aacaaagttc ttagccagaa
     1441 tcgtgaccgc gttcttggcg cggcatagac cggcttcgac cgatgcaaac ccgaggttca
     1501 tgaaaaaaac cagcatggct gcaatcaaca cccacatcgt gtctatggcg actttagcgc
     1561 caccgatttg ctctaagagc ccgttcgtaa attgcaccgg ttcggcgccg ggatcttctt
     1621 tcttgacttc aacgcccgcc ccggacgata ccgccggggc ttctattttg gttggctcgg
     1681 ccggcgctgg gttttcttgc gccgaaagcg agcttgaaag aaagattccc acgacgaaac
     1741 tcagtgacgc aaacgctaac aattttctca gcttcttcat tttgtctcct cctcggagat
     1801 aaactcgatc gtcctaaatc gcatcttcgc ctcgttcgcc ggtgcgaatc cggatgactt
     1861 catccaccgg cgtaacaaat attttgccgt cgccgatctt gccggtgcga gcgctttcgg
     1921 taatcaactc gactaccttt gccgccttat catcagcaac cagtacctca agcctcacct
     1981 tcggcaagaa gtcgacagtg tattccgcac cccgataaag ctccgtatgt cctttttggc
     2041 gcccgaagcc tttgacctct tccacagtca ttccttgtat cccggccttg gttaatgtct
     2101 ccttgatttc ttccagctta aatggcttga cgatcgcctc gaccagcttc atgcaacact
     2161 ccttttcatg gccctgcaaa gatacgtagc aatctgaatc gaaaccgttt gagcaagcga
     2221 gatgccaccg ttctactatt acgagagact cctgcatggg ttaaaatcgc ttaggtaaat
     2281 ttcatggaca cgattagggc gatcccacaa agatgcgcaa tgtgcctgaa gattaggcgc
     2341 tcagcttagt ttctaagcag cgtgacatcg gcaactaata cgcgcggcga gctaa
//
LOCUS       DASUTG010000348         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1240257, whole genome shotgun sequence.
ACCESSION   DASUTG010000348 DASUTG010000000
VERSION     DASUTG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240257"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VF089_07925"
     CDS             <1..421
                     /locus_tag="VF089_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173928.1"
                     /translation="HNGIEKYKINWLTCYKLRPYVHDYWGSCLTCAAVCPFTKPNVWW
                     HSLATWSLRTCPIPARPALVHALKWIDDRFWGITRNNRVRWLGYDSGIKPGDQACTVA
                     GCNASHEGKAPAKEVAGDIGYYAPLKENTNRFVKRDA"
     gene            421..1053
                     /locus_tag="VF089_07930"
     CDS             421..1053
                     /locus_tag="VF089_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173929.1"
                     /translation="MTLRLGPFKLPGLKRDPHKNDPYWDFFINTPPADLANTVTELIR
                     NASPGNVFPTKADIHTPEITSSHLKGMAQYFGADLVGIVKLPANNPSPSPLPHTGDGN
                     IEEEYPFAVVCIVRAEYDPRTAPGIGGQVPVQNGLFITFVLSAWIRELGFHAKVAREI
                     SGEKLAAAAGLGDINRDSRLVTPQYGADVHVADIILTDLPLAPDGSASAP"
     gene            1176..1838
                     /locus_tag="VF089_07935"
     CDS             1176..1838
                     /locus_tag="VF089_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6173930.1"
                     /translation="MTYVITSLCTNDGACVEVCPVACIHTTPGAPQFYIDPEVCIDCE
                     QCEIVCPVDAIFKDIDIPAEHQAALEVNAAFFRKNKAAAGPVSFDTAWEMIRAAHDYA
                     QIMGMAVTAAVVDEAGGPIALGRMDSADPVTAELAFYKAYTAAAFHVATAELAPQARQ
                     PRFRSLTVSHRGRIMPVGGAIPIIDGVTIVGAVGVAGASRPEQDILCCRAALAVIEGH
                     GH"
     gene            1895..2203
                     /locus_tag="VF089_07940"
     CDS             1895..2203
                     /locus_tag="VF089_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173931.1"
                     /translation="MHLIQLLLPLYDNHNVRFPRETFDHVRQELTDRFGGVTAFLRSP
                     GQGFWRESEGAVNRDDVVIFEVMADELDRRWWAEYREELEQRFRQIELVVRASAIERL
                     "
     gene            2448..3911
                     /locus_tag="VF089_07945"
     CDS             2448..3911
                     /locus_tag="VF089_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173932.1"
                     /translation="MKDIRFVDTTLRDGQQSLWAYGMRTGMIVPIADHLDNAGFEAIE
                     LGGPVELPKCVRELREDPWERYRLVYARIKKTPLRVIHGTRSGFAIYPHAIHQLYDRC
                     MAEIGIKQVRISDSWNDPVDWQWRVKQARAAGLEPVINLIYTISPRHTDEYYAQKTRE
                     ALKLNVFRVCLKDPGGILTPERTRTLAPAVLQSAKETIAELHTHCTTGLGSLCCLEAI
                     EAGMTSVNTAIPPLADGSSNPSIFNVARNARAMGYAPVIDEEPLRAVAAHFTAIAKRE
                     NLTIGKPPEYDYAQYIHQIPGGMISNLRHQLRLVGMEDKLERTLDEAAQVRAEFGYPI
                     MVTPLSQFVGSQAAINVIVGERYKEVTDQTIEYALGVWGKEGGALMDPEVKDKILSRR
                     RADEIARRELPQMSLQQLRRKFGGSGISDEEILLRFFTSKEDVDNMRAAGPARLYANG
                     GNPLLHLLEELTRATNRSSISVRRGDVSVHLRRNSAL"
     gene            4155..4793
                     /locus_tag="VF089_07950"
     CDS             4155..4793
                     /locus_tag="VF089_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173933.1"
                     /translation="MENQGFYSLKIGLLLGFSLLLGCVAGPTLHETRRGESLPGPDTT
                     LQPGEIRAEVVQVTPNRNEIQIREADNRTRVLRYDPARTRVTYQGRDYSVENLEAGDV
                     IAFQTFGRDNTIDTIRVQQPVQARAASGSVRPGSGLARGEVLEGTVDRIDYDRGIFDV
                     RTRDRGTITVALPFNARPSDVDAFRRLRNGDSVRLEGEFINRDNFQVSSISR"
     gene            4831..5085
                     /locus_tag="VF089_07955"
     CDS             4831..5085
                     /locus_tag="VF089_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173934.1"
                     /translation="MEQRTGIAATLAIIAAIGGILLIFSGHPGWGLIVELLAIVLGII
                     GFFMSASPRVSGGILSIAAIIIAVFGMGFSLLGIIGGVLF"
     gene            complement(5186..>5641)
                     /locus_tag="VF089_07960"
     CDS             complement(5186..>5641)
                     /locus_tag="VF089_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6173935.1"
                     /translation="HQQRKGENVMAYDIKKVDVWAGEIADRPGGLGATLEALNNAGAN
                     LEFLVSRRAPDRPGTGVVFLTPIKGTKQKNAAQSAGLSTTDSLHSVRIEGPDKPGLGT
                     QLTRALSDAGINLRGVSAAALGRRMVTYVAFDNAADADKAIRALRKALK"
BASE COUNT         1251 a         1574 c         1570 g         1246 t
ORIGIN      
        1 tcacaacggt atcgagaaat acaaaatcaa ctggctcacc tgctacaagt tgcggccgta
       61 tgtccatgat tactggggca gctgcctgac ctgcgccgcg gtttgcccgt ttaccaaacc
      121 caacgtctgg tggcacagcc tggctacatg gtctttgcgc acctgcccga ttccggcgcg
      181 accggcgctg gtgcacgcgc tcaaatggat cgatgatcgg ttctggggaa tcacgcgtaa
      241 taatcgcgtg cgctggctcg gttacgattc gggcatcaag ccgggagatc aggcctgcac
      301 cgtcgccggt tgcaatgcat cgcacgaggg caaggcgccg gccaaagaag tggccggcga
      361 catcgggtac tacgcgccgc taaaagagaa cactaaccgg ttcgtcaagc gggacgccta
      421 gtgaccttgc gattgggtcc cttcaagctg cccgggctga aaagagatcc ccacaagaac
      481 gatccgtact gggatttctt cataaacacg ccgccggcgg atctcgccaa tacggtgacc
      541 gagttgatcc gcaatgcttc ccctggcaac gtttttccca ccaaggccga tatccacacg
      601 ccggagatta cctcaagcca tctcaaagga atggctcaat acttcggcgc ggatctcgtg
      661 ggcatcgtaa aattgccggc gaacaacccg tcaccctcgc ctctgccgca caccggcgac
      721 ggtaatatcg aagaagaata tccgttcgcc gtcgtctgca tcgtccgcgc tgagtacgat
      781 ccacgcaccg cacccgggat tggcgggcaa gtgcccgtgc agaacggact atttattacc
      841 ttcgtgctga gcgcgtggat cagggagctc gggtttcacg ccaaggtcgc gcgcgaaatc
      901 agcggcgaga agcttgctgc cgcagccgga ttgggcgaca tcaatcggga cagccggtta
      961 gtgactcctc aatacggcgc cgatgttcat gtggcagata ttatcctgac ggatctgccg
     1021 ctagcaccgg acggctcggc gagcgcgcca tgaagttagt tccaatggtt ccaaaggttc
     1081 caatcgttcc atcggttcag ataaaacaac tgtggataca aatggaacat ctggaacgat
     1141 tggaaccgtg gtggaggcgc aaggagggga agtaaatgac ctacgtgatc accagtctat
     1201 gcaccaatga cggagcgtgc gttgaagtct gtccggtcgc ctgcatccat acgacaccgg
     1261 gagcgccgca attctacatc gacccggaag tctgtatcga ctgcgagcag tgcgaaatcg
     1321 tctgtccagt ggacgcgatc tttaaagaca tcgacattcc ggcggagcat caagccgcgc
     1381 ttgaagtgaa cgccgccttt ttccgcaaga acaaggcggc ggctggtccg gtttcgttcg
     1441 atacggcgtg ggagatgatc cgcgcggcgc acgattacgc tcagattatg ggaatggccg
     1501 taacggcggc cgtggttgat gaagcgggcg ggccgattgc gctggggcga atggatagcg
     1561 ccgatccggt gacggcggag ctcgcttttt ataaagctta taccgccgcc gccttccacg
     1621 tcgccaccgc tgaactggcg ccacaagcgc gccaaccaag gtttcgcagc ctgacggtct
     1681 cgcatcgcgg tcgcatcatg ccagtgggtg gagctattcc gatcattgac ggcgtaacca
     1741 ttgtcggcgc cgtcggcgtg gcgggcgcaa gccggccgga acaggacatt ctctgttgtc
     1801 gcgccgctct cgcggtgatc gaaggccacg gtcactgacg agtaaccaag aatcgacgat
     1861 tcgtctaaat aactgatctg aaagccgccc gccgatgcat ctgattcaac tcttgttgcc
     1921 gctctatgac aatcacaatg tgcgatttcc gcgcgagact tttgaccacg tccggcaaga
     1981 gttgaccgat cgttttggcg gcgtaacggc attcctccgt tcgccgggac aagggttctg
     2041 gcgcgagtcc gagggtgcgg ttaataggga tgatgtggtg atttttgaag tgatggcaga
     2101 tgagctggac cggcgctggt gggctgaata cagagaagag ctcgaacaac gtttcaggca
     2161 gattgagctc gtcgttcgag ccagcgccat tgaaaggctc tagcctgccg gcgcatgact
     2221 gtttgccttc cacccgctct tccattaagt gtaagtgttt ttctctgctt ccgcttttta
     2281 ctttccttca gaatcatctc tcctccagcg tcccagaatc aaccgggcgc gccgcttcca
     2341 tctgctgtat gcatcgggtg agaaggtgca ggaagcatct ctcgattaaa acctgaactt
     2401 cagcgaacct cttggttcgg catcgaagac cgtttagtat cttttgcatg aaagatatcc
     2461 gttttgtcga tacaacacta cgcgatggcc agcaaagcct ttgggcttat ggcatgcgca
     2521 caggcatgat cgttccgatc gctgatcatt tggataatgc cggttttgag gcgatcgagc
     2581 tcggcggtcc tgtggagctg ccgaaatgcg tccgcgaatt gcgtgaagat ccttgggagc
     2641 ggtaccggct ggtatatgct cgcatcaaga aaacgccgct tagagtcatt cacggaactc
     2701 gcagcggttt cgccatttat ccacatgcga tccatcaact ctacgaccgc tgcatggcgg
     2761 agatcggaat taagcaggtg cgcatctcgg attcgtggaa tgatcccgtc gactggcaat
     2821 ggcgcgtgaa acaagcgcgg gctgcgggac tcgagccggt gatcaatctc atctacacga
     2881 tttcgccgcg gcataccgat gaatactacg cgcaaaaaac ccgcgaagca ctgaaactga
     2941 acgtattccg agtgtgcttg aaggatcccg gcggaatcct tacaccggag cgcactcgca
     3001 ccctggcccc ggcggttctt caaagcgcga aggagactat tgccgagtta cacacgcact
     3061 gtaccaccgg tctgggttct ctctgttgtt tggaagcgat cgaggcgggc atgacgtcag
     3121 ttaataccgc gattcctccg ctggcagacg gctcttcgaa tccctcaatc tttaacgttg
     3181 ccagaaatgc cagggccatg ggctatgcgc cggtgatcga cgaagaaccc ttacgcgcgg
     3241 tggcggcgca tttcacggcc atcgctaaac gagagaattt gaccatcggc aaaccgccgg
     3301 aatatgacta tgcccagtat atccaccaaa ttcccggcgg catgatttca aatttgcgtc
     3361 accagttgcg tctggtcggg atggaagaca aactcgagcg aaccctcgat gaagcagctc
     3421 aagtgcgggc ggaatttggc tacccgatta tggtcacgcc gctctcccag ttcgtcggca
     3481 gccaggccgc catcaatgta attgttggcg agcgttacaa agaagtcacc gatcagacca
     3541 tcgagtacgc cttgggcgtg tggggaaaag aaggcggcgc attgatggat cccgaggtaa
     3601 aggacaaaat cctcagccgg cgtcgcgccg atgaaatcgc cagacgtgaa ctgccgcaaa
     3661 tgtcgctgca acagttgcgg cgcaaatttg gcggatcagg gatatccgac gaggagattt
     3721 tgttgcgctt ttttaccagc aaggaagatg ttgacaatat gcgcgccgca ggtcctgccc
     3781 gactttacgc caacggtgga aatccactct tgcacctgct cgaagagctt acgagagcaa
     3841 ccaatcgctc cagcatatcg gttcggcgcg gcgacgtatc tgtgcatttg cgtagaaatt
     3901 cagccctgta gattcttgta cacacaaccc agacgtctac taggccgcgc gaccgctcgt
     3961 ttatcccaag actactgcct cccaaaatga tggggtgtaa gccgcgttgg gacaggctcg
     4021 tgtacgaatg tgccgccagc ggcacagcgt tttctcatcg cctatccctt ttcgcaaccc
     4081 cctcgaagta gttcattgct cgattccact gtggcacaaa gtttgatttg taagccactg
     4141 caaggaggta cgccatggaa aaccagggtt tttactcgct caaaatagga ttgttactgg
     4201 gattcagcct gctgctcgga tgtgtggcag gaccaacatt gcatgaaacg cgacgcggcg
     4261 agagtctgcc ggggccagac acgactctgc agcccggtga aattagagcc gaagtagttc
     4321 aggttacccc caacagaaac gaaatccaga ttcgagaggc tgataatcgc acccgagtat
     4381 tgaggtacga tcccgctcga acaagagtta cctatcaggg gcgcgattat tctgtcgaga
     4441 atctggaggc cggggatgtc atcgcgtttc aaactttcgg tcgcgacaac acgatcgaca
     4501 caatccgcgt ccagcaacca gtccaagccc gtgcggcttc gggctctgtg cgtcccggtt
     4561 ccggtctagc aaggggtgaa gtgctcgaag gcacggtcga tagaatcgac tacgatcgcg
     4621 gcatcttcga tgtaagaacc cgtgatcgcg gaaccatcac agtcgctttg cctttcaatg
     4681 cccgacccag tgacgtagat gctttccgcc gcctacgcaa tggcgacagc gttaggttgg
     4741 agggagagtt cattaatcgc gataacttcc aagtttcgtc gatctctcga taaatactta
     4801 ccagtagcca tcggggagga gatcatcgtc atggagcaaa ggaccgggat cgccgcaaca
     4861 ctggccatca tcgcagccat cggtggaatt ctactcatct tcagcggtca tcccggatgg
     4921 ggattaatag tcgaactgct ggcaattgtc ttgggcatta tcggtttttt catgtcggca
     4981 tcaccgcgcg tgagcggggg aatcctcagt atcgccgcaa tcataattgc tgtatttggg
     5041 atgggttttt cgttgctggg aattatcggt ggagtgctgt tttagagggt tcaataaaac
     5101 agcgccaccg cagcactgtt gcataatgtt tagccgttcg cggtaactta attacttaag
     5161 gagcgtattc gaagtcgcgg cagaattact tcagcgcttt ccttagggcg cggatcgcct
     5221 tgtccgcgtc cgcggcgtta tcgaacgcaa cgtaagtcac cattcgcctt ccaagcgctg
     5281 ccgccgatac gccacgcagg tttatacctg catcactcag cgcgcgcgtc aattgagttc
     5341 ccaggccggg cttgtccgga ccttcaatcc gaacggaatg caggctgtcg gtcgtgctga
     5401 gcccggcgct ctgagccgca ttcttctgct ttgttccttt tatcggagtt aagaaaacca
     5461 cccctgtgcc gggacggtcg ggagcgcgac gcgaaacaag aaactctaag ttggcacccg
     5521 cgttgttcaa cgcctccagc gtcgccccca acccacccgg acgatccgca atctcgccag
     5581 cccatacatc tacttttttg atgtcatacg ccataacatt ctcccctttt ctttgttggt
     5641 g
//
LOCUS       DASUTG010000349         5343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1092326, whole genome shotgun sequence.
ACCESSION   DASUTG010000349 DASUTG010000000
VERSION     DASUTG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092326"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VF089_07965"
     CDS             <1..449
                     /locus_tag="VF089_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6173936.1"
                     /translation="NNFIKGLKALNVPVHKSTSAMFRLVVAGEYAICMPALLHDVIHD
                     KEKGTPIDYVKSVPPVVFPRQAGIYAKAPHPNAAKLFAEWLISDEGQKTIDRLGRESS
                     RNDFESKTSIEVAFPKGTKAIPVTDKLYLEDPKKWLDTNVKPIWEG"
     gene            778..868
                     /locus_tag="VF089_07970"
     tRNA            778..868
                     /locus_tag="VF089_07970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:812..814,aa:Ser,seq:gct)
     gene            1242..1892
                     /locus_tag="VF089_07975"
     CDS             1242..1892
                     /locus_tag="VF089_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6173937.1"
                     /translation="MMVFRNRRHAGRLLARKLSVYKDKHPLVLGIPRGAVPMARVIAD
                     ELEGDLDVVLVHKLSHPDELELAIGAIDESGSAILTDYAAHVDPEYLELEKQRQLEAL
                     RQRRTRYTPLRSPRDPHGRIVIVVDDGVATGSTMIAALRTVRARTPKELMAAVAVTSP
                     EAARAIARECDAAFCLKVPRKFHAVGQFFTDFAQVEDEEVISALQHGQARSRSAAG"
     gene            complement(2016..3428)
                     /locus_tag="VF089_07980"
     CDS             complement(2016..3428)
                     /locus_tag="VF089_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6173938.1"
                     /translation="MISQNFSGACLLRMCGLVPCRKGLASCLNRTLLTMKRPHTTSIF
                     RNTLEERLTGSVETSTQTLPKRFRLQTFSSLAYLDYRYLWTGTLMMSAGQWVQQVALG
                     WLVYELTGSSVLLGLLNGLRALPFLVTGPIAGVAADRMDRRQLMLRTQYVLIVTAFLM
                     GALVASGWLQVWHIFVFTLITGIGWSFSEPVRQSLIPSVVPKAELANAIALNSAGFNL
                     MKVIGPALGGVMIAVFGAAGNFWVQGAAYVVVLYMIQLMHVPPTRADVLRASAMANLK
                     EGFAYVRSTPTVLALMILAYVPRVFAVPYQTLMPVFQKDVLQVGPEGLGLLMAAPGLG
                     AVLAVLTLASISSRIKRQGLTLIASTIMLGLSLILFSQIKTFHLALIVLVIAGAFQMV
                     FLASTSTMLQLIVPDELRGRVMSLYMLDRGLMPAGALFAGVVAHLVGAPLAVTSMGAI
                     VVVLTLLVAWRVPHIRKLQS"
     gene            3857..4456
                     /locus_tag="VF089_07985"
     CDS             3857..4456
                     /locus_tag="VF089_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173939.1"
                     /translation="MKKLSKIAALAGVLACVDIAAAAAQTLEETFYRQWVEYRDEHLS
                     VAFHQTPVVIALQEIRQKTGFQFSLPSSADGKLLNLRLDRSPLEPALYSLLSNIGFRN
                     FALIYDERGRPNRAVVLGSQIDEALGLMADSSQTTEAVVQPLKSEERDTLQKELERWS
                     ELKQEERGRIEDRLKTLPPSEEREQLVKEYGRKLLGIKN"
     gene            complement(4698..>5343)
                     /locus_tag="VF089_07990"
     CDS             complement(4698..>5343)
                     /locus_tag="VF089_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEX6173940.1"
                     /translation="DTLEPETNLDTVEKVVGEVVKQIDGDLSISPTQLNKAIGKYLAE
                     HYPKDRGILKSAYLHDVPVFVPAFVDSELGNDLYIHNVRRRRSGKQPILMDLELDSKL
                     LIELVTKNKRFGILSIGGGVPRNNVQNVAPLIEIINERLGKTYPERRFTYGIRVCPDR
                     PHFGHLSGCTYSENESWRKAVKNGIYAEVLADATQVWPFLVKYLMEKGKVRGTK"
BASE COUNT         1271 a         1372 c         1463 g         1237 t
ORIGIN      
        1 tcaataactt catcaaggga ctcaaggcgc tgaacgtgcc agtgcacaaa agcacgagcg
       61 ccatgttcag gcttgtcgtc gccggcgagt acgcgatctg tatgcccgca ttattgcacg
      121 acgtgattca cgacaaggag aagggaacgc cgatcgatta tgtaaagagc gttcctcctg
      181 tcgttttccc gcggcaggcg ggcatttatg ctaaagcgcc ccacccgaac gccgcgaaac
      241 tttttgccga gtggctgatt tcggacgagg ggcaaaagac gattgaccgt ttaggccgag
      301 agtcgtcccg gaatgatttt gaatccaaga cctcgattga ggtcgcgttc cctaaaggta
      361 ccaaggcaat cccggttacc gacaagctgt accttgagga tcccaagaag tggctcgata
      421 ccaatgtgaa accgatctgg gaaggctgaa gtctcaattt gacttagctt ggagaaaagc
      481 gggtgcaact gcggtggacc agctttgatc tatccgcgct gctctgacat atcggaagaa
      541 aaagacttcg acctaggcgc tgcgaaatac tgttagcttt acgtgagcga gatagtccgc
      601 attgcaaaca taaaaagttt ggtcgtgcgg gtgaaccacg ctgtcgggtc ggattgtgga
      661 atccggcaat gtcgtatgtt aatttggtat ttgctggggg gtgtgtctaa ccattgctga
      721 ggatcaaagc ttaattcgtt tctcggtgtt ctgtcgagaa acaacagccc gcattaagga
      781 gagatggccg agtggtctaa ggcgcacgcc tgctaagcgt gtgtacctca aaagggtacc
      841 gagggttcaa atccctctct ctccgccatt ttttttccac atcttaagcc aaaacttggg
      901 cgagtgggta tttccgatca taatccatgg tcggagcatg agcgcctaga gaagtaggcg
      961 tgtgctaagt accgcaatcg aattttcggc accatgccgg tgcgccgact gtggttatgc
     1021 gattgctgca cggcagaacg gatgcgttcg cgtgaatacg aggcttgact ttaagcaacg
     1081 ggtgagtacg gcgatcttga tattgcgatt cgtatccagt ttagatgaga gagcggacgc
     1141 ccgggcgcca cggctgaact tttctcgata agagtcgagt accgagattg cggttggcta
     1201 agttgaactt gctctaagca agcggatgta aacatcggca gatgatggta tttcgaaacc
     1261 ggcgccacgc gggccgctta ctggctcgca agctgagcgt ctataaagac aagcacccgt
     1321 tggtacttgg aatcccgcgc ggtgccgtgc cgatggccag ggtcattgct gacgagctcg
     1381 aaggcgattt ggatgttgtg ttggtgcata agcttagcca tcccgatgaa ctcgagctgg
     1441 cgattggcgc cattgacgaa agcggtagtg cgatcctaac cgactacgcc gctcacgtgg
     1501 atccggaata cctcgagttg gaaaaacagc ggcagcttga agctttgcgc cagcgtcgga
     1561 cccggtacac acctttgcgt tccccgcgcg atcctcatgg ccgcattgtg atcgtggttg
     1621 atgatggcgt tgctacgggc tcgacgatga ttgcggcgct gcgaacagtc cgtgccagga
     1681 caccgaagga gctcatggcg gccgtggcag tgacttcgcc cgaagcggcc cgggcgatag
     1741 cccgtgaatg tgacgctgcg ttctgtttga aggtgccacg caaatttcac gcggtggggc
     1801 agtttttcac cgactttgct caggttgagg acgaagaggt gatctcggca ttgcagcatg
     1861 ggcaagcgcg gtcgagatca gcggcaggtt aaatttcacg tcttcgttca aacaatcgcc
     1921 tgtgctggac ctagagcacg tccgcgtaca ttgcgactgt atgcccagct tcagtgcttg
     1981 cttccttgat agacaagatc agatcttgac tccggctagc tctgaagttt cctgatatgc
     2041 gggacgcgcc acgctactag caaggtcagg acaacaacaa tcgctcccat cgaagtgacg
     2101 gctaacgggg caccgacgag atgcgcgaca actccggcga acaatgcgcc tgcgggcatc
     2161 agcccgcggt caagcatgta caggctcatc actcgacccc gtagctcgtc gggaactatg
     2221 agctggagca ttgtgctggt acttgcaaga aacaccattt gaaatgcgcc ggcgatgact
     2281 aaaacaatga gcgccaaatg aaatgttttt atttgggaaa atagtataag tgacaacccg
     2341 agcataatcg tgcttgcgat tagcgttagt ccttgtcgtt tgatcctgct cgatattgat
     2401 gcgagcgtca gaaccgccag caccgctccc aggccggggg cagccatcaa caggccgaga
     2461 ccttcaggcc ctacttgcaa aacgtccttc tggaacaccg gcatcaaggt ctgataagga
     2521 accgcgaaca cgcgcgggac gtaggccagg atcatcagcg ccagcacggt cggtgtcgag
     2581 cgcacatatg caaacccttc tttgagatta gccatagcgg aagcgcgaag cacgtcggcg
     2641 cgggtcgggg gcacgtgcat taactgaatc atatacagaa caaccacata cgccgcgccc
     2701 tgcacccaaa aatttcctgc ggcgccgaac acggcgatca tcacgccgcc gagcgccggc
     2761 ccgataactt tcatgagatt aaacccggcg gagttgagcg caatggcgtt agcgagttcc
     2821 gctttaggaa cgacgctggg aatcaggctc tggcggacgg gttcgctaaa cgaccaaccg
     2881 attccagtga tcaaggtaaa gacaaaaata tgccacacct gtaaccatcc ggatgcgaca
     2941 agtgcgccca tgaggaatgc tgtcacaatc agcacgtatt gagtacgcag catcagctgc
     3001 cgccggtcca ttcgatcggc ggctacaccc gctatcggtc cagtcactaa aaacggcagg
     3061 gcgcgcagcc cgttcaataa tcccaacaaa accgaagagc cggttagttc gtagactagc
     3121 cagccgagtg ctacctgttg aacccactgc ccggcgctca tcatgagagt tccggtccaa
     3181 agatagcggt aatctaggta ggcgagggac gaaaaagttt gcaatcgaaa acgcttaggg
     3241 agtgtttgcg ttgaagtttc aacggagcct gtgagccgtt cttccaaggt attcctgaaa
     3301 atcgaggttg tatgagggcg cttcattgtt agcagagttc ggttaagaca gctagcaaga
     3361 cctttgcggc acggaactaa gccgcacatt cgtaacaaac aggccccgga aaaattttgg
     3421 ctgatcatag acgggctctg caacttcggc acgattttat cgaagctaac tgcgcagcag
     3481 agcgtcgact cttgctcata cgacctgaag gctcctgcaa gaaaacagaa gatcacttct
     3541 ttgaggccgt ttgccgcatt gctgtcgtag atcgaggcga cgagcgttac acgaagaaag
     3601 gacgttactc gttcgagcga attagtaaac cgcagcacga gttccttctt gaagatagcc
     3661 ggctcaaagc tacatcggat attcctccca gtcgtttggg tgcttcagtc tttgtgctac
     3721 aaaagtgttc ttgttttcgc ttcagggtta aagccttgag cgataaaagc tctcaagttg
     3781 attgatttta tgaccatttt agattgcacg agacggggca caagacttgc tgccttggcc
     3841 ataaatgagg tgatcgatga aaaagctttc caaaattgcg gccctcgcag gggtcctagc
     3901 ctgtgtcgat atcgcagcag ccgccgcgca aaccttggaa gaaaccttct atcggcagtg
     3961 ggttgagtat cgcgacgaac atctttccgt tgcgttccat cagactcctg tcgtcatcgc
     4021 gcttcaggaa atccggcaga agaccgggtt tcagtttagt ctgccgtcat cagctgatgg
     4081 taaattgttg aacctccggc tggaccgttc gccgctggag ccagctctgt attctcttct
     4141 ttctaatatt gggtttagga actttgctct catttatgat gagcgcgggc gccctaaccg
     4201 ggcggtcgtg ctgggatctc aaattgacga agcgctgggc ctcatggcgg attccagtca
     4261 gacgaccgaa gcagttgttc aacctctaaa gtcagaagag cgcgacacgc tgcagaagga
     4321 gttggagcgt tggagcgaac tcaaacagga agagcgcggg cggattgaag accgattgaa
     4381 aactttgccg ccttccgaag agcgcgagca actggttaaa gagtacggcc ggaagctttt
     4441 aggaataaaa aattagctga tttcggaacg cctttaacct gctgattctg gcaggttggt
     4501 tcaaccgcat cctcgcttgc taagcatccc ttaccgtgca ttatcctgcc tcgcactaac
     4561 tacggattat taagcgcgtt gcatccatcg gcccgtttat tcccgcttca gtaaatctct
     4621 aaggtccaga tgcacctcgc tcctgccaga gaaattttta tgtatcagat ttgattacag
     4681 cggcggcgac ccatcgatta cttcgtgccg cggacctttc ctttctccat aaggtacttt
     4741 accagaaatg gccaaacttg agtcgcgtcc gctaagactt cggcatagat gccgttcttg
     4801 actgctttac gccaggattc gttttctgaa taggtgcagc cggagaggtg accgaaatgg
     4861 gggcggtcgg gacatactct tattccatag gtaaaccggc gctccggata cgtcttgccc
     4921 aaccgctcgt tgatgatctc gattagtggc gcaacgtttt gcacattgtt tcgcggcacg
     4981 cccccgccaa tggataatat accgaaccgc ttattctttg tgaccagctc gattaacaat
     5041 ttgctgtcga gctctagatc cataaggatc ggctgctttc cgctgcgcct gcgccgaacg
     5101 ttgtgaatat aaagatcgtt gccgagttcc gagtcgacaa aagcggggac gaataccggc
     5161 acatcatgaa ggtaagccga tttaagaata ccgcgatctt tcggatagtg ttcggcgagg
     5221 tatttgccga tcgccttatt tagctgggtc gggctaatcg agagatcgcc atcgatctgc
     5281 ttgaccactt cgccgaccac cttctcgaca gtgtctaaat tggtttcagg ctcgagcgtg
     5341 tcc
//
LOCUS       DASUTG010000350         4085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1981666, whole genome shotgun sequence.
ACCESSION   DASUTG010000350 DASUTG010000000
VERSION     DASUTG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981666"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF089_07995"
     CDS             complement(<1..312)
                     /locus_tag="VF089_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173941.1"
                     /translation="MSSAPIHVLLIEDNPGDARLLRALLQETGSSQFQLVHAERFSDA
                     LTRLSERRFDVVLLDLSLPDAQGIETISRLGGQADGTPIVILTGLNDEEIAIRALQQG
                     AQ"
     gene            complement(309..761)
                     /locus_tag="VF089_08000"
     CDS             complement(309..761)
                     /locus_tag="VF089_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173942.1"
                     /translation="MSVYNGCIQVLLVEDNPGDVRLTKEALKEGKLLNQLTVVGDGVE
                     ALSFLRKEGIYADALQPELILLDLNLPKKDGREVLAEIKADPNLRRIPVVVLTTSSSE
                     EDILKIYDLHANCYITKPVDLEQFMGVVKSIEDFWVSVVKLPSHEAAK"
     gene            complement(758..>4085)
                     /locus_tag="VF089_08005"
     CDS             complement(758..>4085)
                     /locus_tag="VF089_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6173943.1"
                     /translation="LYQDLTSYALVLVYLVIYHAVVGALWPHYVYNHPAALAAPWSWA
                     AVHSFFLIAAAIGNMSAWRFNQKAFAQASLILNSAGEGIYGVDLQQRILFMNSSAAEL
                     LRCQADEVLGKPMQQILRHTRADGTEFSRQPSSIVAALRDGAKHQATDELFWRNDGTS
                     FPVEYKCAPIFERNALAGTIVTFTDISIRKQREAALRESEERFRQIAEGINEVFWVTD
                     SVNNQKLYISPAYREVWGRDPGKIGSLSQSWLGLLHEDDRLRVLAAVSTKQLTGQYSE
                     EYRIVREDGSIRWIRDRAFPVRSESGAVYRMVGLAEDITERKQAQQALQDRYTEMAVL
                     HDVSQTILSATDLEPVLQNIVDRALATLSLDIGNIRLFEPVGRMRIGIFRGYRDYENT
                     RVYAEMKNPRSDALSMRIIESGKSLVLENLTPADGLLAFTKEQVCSALVVPIILERET
                     LGLMEMGSRTASTFEPRDVRLLTAIGNQVGLAIQKARLLHETERRANEQAALNVIAKA
                     ISQSLRTDELLKIALEKVIEVTGRERLSIRLKDSASDGMRLVAHRGFAPDEIEELVRP
                     SSDSVIREVFASGQPRVINNSPAIGNSEALSPQKNPAGMIPIKAGTHVVGILSVSGIR
                     PVPFELRELELLEAIGNMIGVALENARLFSETEARYRELKTLQAITDTILETLDVRVM
                     MERILDKAFEIGRFDCGVIRLLDRDHETLKLVASRGHRDREGVLNHYETSEQKSTARI
                     IAEVMASKQTKVKDLSTSDGMRTFRREGVCTVVAIPLHTQHDVLGIIQLGSRIVREFQ
                     ESELRVFDAIGGQAGIAIQKARLYEESQQAQAALREKAAELTRSNADLQQSTQEVKSA
                     KEKLQRVNSVLTVQAAELARSNTELEQFAYVASHDLQEPLRMVASYVQLLARRYKGKL
                     DSEAEEFIGFAVDGSKRMQDLIQALLAYSRIGTKGREFAPTNCEVVLRNALKNLQIAI
                     EDSHARVTHDPLPTVKGDAIQLGQLFQNLIGNAIKFRGEKPPSVHVTAERQGSDWLFS
                     FRDDGIGIDAQYADRIFVIFQRLHTKEEYPGTGIGLALCKKIVERHSGRIWVESEPQK
                     GSTFRFTLPAEHSAEGDKQL"
BASE COUNT          894 a         1183 c         1027 g          981 t
ORIGIN      
        1 ttgcgctccc tgttgcagcg ctctgatggc aatctcctca tcattgagac cggtcaaaat
       61 cacaatcggc gtgccgtccg cctggccgcc caaacggctg atcgtctcga taccctgcgc
      121 gtcgggaagc gaaagatcca ataatactac gtcaaatcgg cgctcgctca gccgcgttag
      181 ggcgtcgcta aaacgttccg catgaaccag ttggaattgc gagcttcccg tctcttgcaa
      241 gagcgcgcgt agaagtcggg cgtcgcccgg attgtcttcg atcaacagta catggatagg
      301 cgcgctgctc acttagccgc ctcgtgcgac ggcaacttga cgacgctcac ccagaaatct
      361 tcgatggact tcaccactcc cataaattgc tcgagatcca ccggtttggt gatgtagcag
      421 ttggcatgca aatcgtagat cttgagtatg tcctcttccg aggaagaggt tgtaagcaca
      481 acgacaggaa ttcggcgaag attgggatcg gctttgattt cggcgagaac ctccctgccg
      541 tccttttttg gcaaattcaa gtcgagaaga atcagttcgg gttgaagcgc atcagcgtat
      601 attccctcct tacgaagaaa tgagagcgcc tccactccat cgcctacgac cgtcaattga
      661 ttcaacagct tgccttcctt gagcgcttct ttcgtcaatc gaacatcgcc gggattgtct
      721 tccaccagca gcacctggat gcagccgttg taaacactca taactgttta tctccttccg
      781 cggaatgttc cgccggcaac gtaaaacgaa aagtcgagcc cttttgcggc tctgattcca
      841 cccagatccg cccgctgtga cgttcaacga tttttttgca caacgctagg ccgattcccg
      901 tcccgggata ttcctccttg gtatgcaacc gctgaaagat gacgaagatc cgatccgcgt
      961 actgagcgtc gatcccgatt ccatcgtcgc ggaatgagaa cagccagtcg ctaccctgcc
     1021 gctcggccgt cacatgcacg gaagggggct tttcgccgcg aaatttgatt gcattaccga
     1081 tcaggttttg aaatagctgg ccaagttgaa tggcatcgcc cttaaccgtc ggcagcgggt
     1141 cgtgagttac cctcgcgtgg ctatcttcga tggcgatctg cagattcttg agagcgtttc
     1201 gaagaacgac ctcacagttt gtcggcgcaa actctcgacc cttggtccct atacgggagt
     1261 aggccagcaa agcctgtatc aggtcctgca ttcgcttgga cccgtccacg gcaaaaccga
     1321 tgaattcttc ggcctcgcta tcgagtttgc ctttgtagcg gcgggcaagc agctgcacgt
     1381 agcttgcgac cattcttaac ggctcttgta aatcgtgtga cgcgacatag gcaaattgct
     1441 ccagctcggt attggagcga gccagttccg ccgcctgaac cgttagtaca ctattcaccc
     1501 gttggagttt ctcttttgcc gatttaactt cctgtgttga ttgctgcaga tctgcattcg
     1561 aacgagtcaa ctccgcagcc ttctctctca atgccgcctg cgcctgttgg ctttcctcgt
     1621 aaagccgagc cttttgtatt gcaattccag cctggccgcc gatcgcgtcg aaaactcgta
     1681 actcactctc ctgaaactct ctgacgatcc ggctaccgag ctgaatgatg ccgagcacgt
     1741 cgtgctgggt gtggagcgga atagccacca ccgtgcacac tccttctctt ctgaacgtgc
     1801 gcatgccgtc tgatgtactc aggtctttta ccttggtctg cttgctagcc attacttccg
     1861 cgataatcct tgcggtggat ttctgctcac tggtttcata atggttgagg acgccttcac
     1921 ggtcgcgatg tccacggctg gccaccagtt tcaacgtttc gtgatcgcgg tctaacaggc
     1981 ggatcacacc gcaatcgaac ctgccgatct caaaagcctt gtcaaggatt cgctccatca
     2041 tgacccttac atcaagagtt tcgaggatgg tgtcagtaat cgcctgtagc gtcttgagtt
     2101 ctcggtagcg tgcctcggtt tcactgaaca gtcgcgcatt ttcgagcgct acaccaatca
     2161 tgtttccgat agcctcaagc aattccagct cgcgcagttc gaaggggacc ggtcggatac
     2221 ccgacacgct caaaatcccc accacatgag tgccagcttt tatcgggatc atgccggccg
     2281 gatttttttg cggggacaat gcttcagagt tgccgatggc gggagagttg ttgatcactc
     2341 gcggctgacc gcttgcaaac acctctcgga taacggagtc ggatgacgga cgaaccagtt
     2401 cttcaatttc atccggtgca aaaccccggt gcgccaccaa tctcatgccg tcactagcgg
     2461 aatctttgag ccttattgac aggcgctctc gtccggtcac ttcgataacc ttctctaacg
     2521 caatcttcag cagttcatcg gtacgaagcg actggcttat agctttggcg ataacattca
     2581 gagctgcttg ttcattggcg cgccgctcag tctcatggag aagccgcgcc ttctgaatgg
     2641 caaggcctac ctggttgccg atcgcagtca gcagacgaac gtctctgggc tcgaacgtcg
     2701 aggctgtgcg gcttcccatc tccatcagcc caagcgtctc tcgctccagg atgatcggca
     2761 ccacaagagc cgagcacacc tgttcctttg tgaaggcgag tagcccgtcc gctggggtaa
     2821 gattttcgag cactaaactc tttcccgact caatgatccg catggaaaga gcatcgcttc
     2881 tggggttttt catctcggcg taaacccggg tattctcata atcgcgatag ccgcggaaaa
     2941 ttcctattcg catacggcca acgggctcaa acagccggat attaccaatg tcgagcgaga
     3001 gagtggcgag cgctcgatcg acaatgtttt gcagaacagg ctcaaggtct gtggcgctca
     3061 aaatcgtttg gctcacatcg tgcagaaccg ccatctcagt gtaccggtcc tgcaaagctt
     3121 gctgtgcctg cttacgttcc gtgatatctt ccgctaagcc gaccatgcgg taaactgctc
     3181 cagattcgct tcgcactgga aaggcgcgat ccctaatcca gcgaatagat ccgtcctcgc
     3241 ggacgatgcg atactcctcg ctatactgcc ccgtaagctg tttggtgctc accgctgcaa
     3301 gtacccgaag gcgatcatct tcgtggagta atcccagcca cgattgagac aaagagccta
     3361 tcttgccagg atcgcgtccc caaacttctc gatatgccgg gctgatgtaa agcttctggt
     3421 tgttcaccga gtccgtaacc caaaaaacct cgttaatgcc ttcggcaatc tggcgaaacc
     3481 gttcttcgct ctcgcgcaac gccgcctctc tttgctttcg gatgctgatg tcagtaaaag
     3541 taacaatcgt tcccgccaat gcgtttcgct cgaagatagg agcgcacttg tactccaccg
     3601 ggaagctcgt accatcgttg cgccaaaata attcatcagt ggcctggtgc ttggcaccgt
     3661 cccgtaaggc ggccacaatc gacgacggct gtctcgaaaa ctcagtgccg tcagcgcgcg
     3721 tgtggcgcaa aatctgctgc atgggttttc caagcacttc atcggcttga caacgcagga
     3781 gttcagcggc cgacgagttc atgaagagaa tcctttgctg caagtcaact ccgtaaatcc
     3841 cttcgccggc agaattgagg attaaactgg cttgtgcgaa tgccttctga ttgaagcgcc
     3901 acgcggacat gttgccgata gcggccgcta tcaaaaagaa tgaatggaca gccgcccaac
     3961 tccacggcgc ggccaaagcc gccggatggt tataaacgta gtgcggccat agcgcaccga
     4021 cgacagcgtg gtaaatcacc agataaacaa gaaccagggc atacgaagtg agatcctggt
     4081 acagc
//
LOCUS       DASUTG010000351         9117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1981680, whole genome shotgun sequence.
ACCESSION   DASUTG010000351 DASUTG010000000
VERSION     DASUTG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981680"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1313
                     /locus_tag="VF089_08010"
     CDS             192..1313
                     /locus_tag="VF089_08010"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173944.1"
                     /translation="MNESRKETDTGKLGAGLRPSCGFVASVFLRHRLAGNAILMIGMA
                     VRLLFSAEAGAASLRSAASSMTQAQVTLAQASPRASRPLRVAYLSTSATMASLWMAKE
                     IGGFEKAGVEVEVLSMASSVAIPALIANEIDAVQVSAAPVITASLRGHDVVFVAGLLN
                     TMIWDFYARPEIKSPEQLKGKLIGTDRPATPVAYGTLVALKKMGLTPKDVQLFPLGSS
                     AQVVAALHSGQIIGGLGAPPASFVLERAGFRSMVNLLDVPYQNVGIVVRRSRMDELGG
                     RLVPLLRAVRAGIDRYYSDKAFAMKVIGKYTKEADADVLNKTYEFYRRAGFRRELVIS
                     EPGVQGILDFLSETIPEAKKATPGQFFDDRFVRQVNSGK"
     gene            1405..1995
                     /locus_tag="VF089_08015"
     CDS             1405..1995
                     /locus_tag="VF089_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173945.1"
                     /translation="MTTSKRLPAFEKFIQQLRAAWSELPDMQARMKKGASLLEELVKD
                     PTVKAAAKSWPSTEGRKNLLFYEDPDFNFAINGVVRIPGRKGSIHDHAHAWTAYGILD
                     GTEMLERFRRADDRSKQGYAKLELESVTEGKPGKVDLVPPFDIHAEQGGPTRSVAIIL
                     RSERVAGKTTQGSYDIEQQTIRQIDGPIQVPYEITG"
     gene            complement(2604..3311)
                     /locus_tag="VF089_08020"
     CDS             complement(2604..3311)
                     /locus_tag="VF089_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173946.1"
                     /translation="MNEITGYVVRVAEHFRSELGPSLVEVYKLGSLAHGGFSGIYSDI
                     DVGLLLNCKEPPSEFPTLVTQAKSLHPDLGKKLSVFWGNPEFSWGRLPVIDRLDLLDH
                     GVPLLGEHKADFPRPIKVEIHQSLRESIEKSWQPRIPELQRLSRLEAKDRKPYVRAIL
                     YAARLIFTWDKLAVDSNDRAVEYLHQVQPPGLDLEPIDRALACRRDQCTAEELFALRT
                     DLSRQVRSAVSYISKRT"
     gene            complement(3353..5431)
                     /locus_tag="VF089_08025"
     CDS             complement(3353..5431)
                     /locus_tag="VF089_08025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX6173947.1"
                     /translation="MQNAEFFNLYNHGLIRVAVGIPELQVADPAFNGSRTIELMEKAE
                     RDKAILALFPELGLSAYSCDDLFHQQALLDGSLDALRMILKASEALKLIAVVGMPLRI
                     DHLLFNCAVVLSRGKILGVIPKTFLPNYREYYEHRQFAPAAAACSTEVEIFDHTGIPF
                     GAHLLFQADRQREFTFCVEICEDLWVPIPPSSYAALAGATVILNLSASNITIAKDEYR
                     HNLVSNQSARCIAAYLYTAAGTGESTTDLAWDGHAMVYDSGVLVGESERFRYDSQLVL
                     AELDLERLALERMRQNTFGQSMQLHRDELRRFRKVTFSLETPKMGRLLCRRKIDRFPY
                     VPSDPAVRDLRCYEAYNIQVQGLVKRLKSSKISKIAIGISGGLDSTHALVVAARSMDV
                     LKLPRSNILAFTMPGFATSQHTLDNANALMAAIGCESHELDIRPSCEQMLRDIGHPYA
                     AGQPVFDVTFENVQAGERTSHLFRLANLHQALVLGTSDLSELALGWSTYGVGDHMSHY
                     AINASVPKTLMQFLIRWVAETNQLGEQSSRVLRAILETDFSPELIPAEDDGEPFAQKT
                     EDVIGPYELQDFTLYYTIRFGYAPSKIAFLSYCAWRDRVQGHWPDVPEPKRNQYTIGE
                     IKHWLSVFAWRFFQLSQFKRSCIPNAPKVGSGGSLSPRGDYRAPSDSEATVWLEEIKN
                     IPDQDSEAGS"
     gene            complement(5632..6720)
                     /locus_tag="VF089_08030"
     CDS             complement(5632..6720)
                     /locus_tag="VF089_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6173948.1"
                     /translation="MLLINNQEVEKLFDMKACLAALEDAYDDLLIGDAVYRPRLDLWM
                     PCDRPDGYWRWGTMEGASRKIGVFAIRMKSDVVYWPNGDTEEKYCMQPGTWCGLVMVF
                     SVRNGEPLAIINDGLMQHMRVGGCAGLGVKYLAREDASDVGIFGSGGMARSYLEAFYE
                     VRKLKTVRVYSPTKANRESFAREMTGKLDLEVVAVDAPEKVVRGADIVATCTDSTRTV
                     FDEPEWLKRGAHITCVRACEVGPKVIQKCDLSVKLGKNTIEAMDEGMIRLHGNVGYIA
                     GQPEERSRIPNPTVDNYKGDYFKYFMDVRAGKVPGRTSNEQVTFFINAGTQGLQFAAC
                     AGKVVELAKQKKVGRALPTEWFLQDIRD"
     gene            7020..7682
                     /locus_tag="VF089_08035"
     CDS             7020..7682
                     /locus_tag="VF089_08035"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX6173949.1"
                     /translation="MTLRSFREVFFGATRDSFNSVRTIPMVMVAAIMFAGIAGAQNSE
                     TDALEQQPFFSARLSGFNEVIFVAGQAGPPLVPPALRGAISTPANGRFTAILDSPDLI
                     QYELSYEGLVAPVTQAHIHFGQRHTVGGIIVWLCQTAGTQAPEAVRSSTPLCPSEGTV
                     SGTITPAQVLAQDAQGFAAGDFDELVRAIRNRAAYANVHTEKFTAGEIRGQIRSNRNR
                     DN"
     gene            7939..8331
                     /locus_tag="VF089_08040"
     CDS             7939..8331
                     /locus_tag="VF089_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173950.1"
                     /translation="MVIRIASAVLTMAGLLALISGILFWTGTAVNLIALHMLLGFLAV
                     GALWVIGLTQAFAQHGSWIIAACALIVGAAMIVLGMMQSSLLPGEYHWIIQAIHLALG
                     LLTIGIGHMAAARYRKASVGEQSTPQRH"
     gene            complement(8472..>9117)
                     /gene="tenA"
                     /locus_tag="VF089_08045"
     CDS             complement(8472..>9117)
                     /gene="tenA"
                     /locus_tag="VF089_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX6173951.1"
                     /translation="RRRSERIWQRIESHPFLRELHAGTLPMDRFTYFILQDYVYLLDF
                     AQVLCLGAAKSADLATLELFARHALGAVEVERSFHASFGKTLGLSREQLDAVQKGPIT
                     QAYSGHLQSVARSGSLSEIVAAVLPCYWIYGEAGRRLKKGAPGKPKIYREWIEIYASE
                     EFWKPVREQIQLMDILGAEAKPGEKKLLVEHFILSSRYELMFWEQAYRLEQWPI"
BASE COUNT         2123 a         2505 c         2455 g         2034 t
ORIGIN      
        1 caatcggatg gaatgggaga tcgacgcagc gcgaagagac gagcaagcta gccggaatag
       61 ggagtaatgc ggttaacttc ataaatcgct tcacccgctc atgcgaagta agctctattc
      121 ccgttcacaa ggtgatacgc ctattttgat ccaaagaaat tgcagattct gattcgtctg
      181 atgagcacac aatgaacgaa tccagaaaag aaacagacac gggaaaactt ggagccggct
      241 tacggccgtc ttgcggcttt gtcgcatcag tttttttgcg gcatcggctc gctggcaacg
      301 ctatattgat gatcggcatg gccgtacgat tattattcag cgctgaagcc ggcgccgcct
      361 ctttgcgatc ggcggcatct tcaatgactc aggcgcaagt caccctggcg caagcatctc
      421 ctcgggccag ccgacctctg agggtggctt atctcagcac gtcggcgact atggcgtcgc
      481 tgtggatggc taaagagatc ggcggcttcg agaaagcggg cgtcgaagtc gaggtcttgt
      541 caatggcatc gagtgtcgcc ataccggcac tcatcgccaa tgagatcgat gccgttcaag
      601 tctcggccgc gccggtgatt accgcttcct tgcgcggtca tgacgtcgtc tttgttgcgg
      661 gactgctcaa cacgatgatt tgggactttt acgcgcgccc ggaaataaaa agcccggaac
      721 aacttaaagg caagctaatc ggcaccgatc ggccggcaac cccggtggct tatggcaccc
      781 tggtagccct caaaaaaatg gggctcacgc cgaaagacgt tcaacttttt cctctcggca
      841 gcagtgccca ggtcgttgcc gctctccaca gcggtcagat tatcggcggc ttgggcgcgc
      901 ctcccgccag ctttgtcctg gaacgtgccg gctttcgatc gatggttaat ttgctggatg
      961 ttccctatca gaatgtcggc atcgtcgttc ggcgcagccg catggacgag ctcggcggac
     1021 gcttggtgcc gctcttgcgc gcggttcgcg cgggcatcga tcgctattac agcgacaagg
     1081 cattcgccat gaaagtcatc ggcaaataca ctaaagaagc cgatgcggac gtgctcaaca
     1141 agacctatga attttatcgc cgcgccggat ttagacggga gctggtaatt tcagagcccg
     1201 gtgttcaggg cattttggat tttctgtccg aaaccatccc cgaggcaaag aaagcgacac
     1261 cggggcagtt tttcgatgac cggttcgtgc gtcaagttaa cagcggcaaa tgatcttgtt
     1321 tgtagtcaga gccgatagca gatttggaat gggcaacaaa actccgggct ttaaacgata
     1381 gttaccagcg ggtatagagg tcctatgaca acttcaaaac gacttccagc cttcgaaaaa
     1441 tttattcagc aactgcgcgc tgcgtggtcc gaattgccgg acatgcaagc tcggatgaaa
     1501 aagggcgcga gcctgctgga agagctcgtc aaagatccta ccgtcaaggc ggccgcgaaa
     1561 tcctggcctt caaccgaagg acgcaaaaat ctactcttct acgaagatcc ggacttcaat
     1621 tttgcgatca atggtgtcgt ccgtatccca ggtcgcaaag ggagtatcca cgatcacgct
     1681 catgcctgga cggcttacgg tattttagac gggactgaaa tgctggaacg attccgccgc
     1741 gccgatgatc ggagcaaaca aggttatgcc aagttggagt tggaaagcgt caccgaagga
     1801 aaaccaggca aagtcgatct ggttccgccg ttcgacatcc atgcggaaca gggtggcccc
     1861 accagatcag tcgctatcat cctccgctcc gaacgggtgg ccggaaaaac aacgcagggc
     1921 agttatgaca ttgaacagca gacaatccgg cagatagacg gcccgatcca ggtgccctat
     1981 gagattaccg gctgagcgca agcgacgccg tcggcatcca ccatggatta gagatatttc
     2041 tccacactgc tgagcctttt aagcgattgc gcgttgatca ctactcagtc acttgtgtat
     2101 tcgcgccagt tgatcggccg gcgtggttga tcctcataaa agctggcgct gtcacgtaat
     2161 accgcacatc cggaagccaa gtgaacggcc aagagcaacc aagtagcttt tcttgtggca
     2221 tttacgaaat tggcagttgc agttttcatt aaacgtctcc tgccgcggtg atggatcgtc
     2281 caattaagat ggcaattcgg atgccagtcg agcaatgcca acccttgaat gaaacccatt
     2341 gccttctcgg tggattcaca gccctccaga tgcgagcacc ggatctggtg aaactttaga
     2401 tacagagtgg aaacacggtt gctagattta gttgttcggt gggaatgagc gcctggagat
     2461 gccggcgcga gcttaaccga ttggagcggc ttcttagtcg gcacggaaac tctggaccca
     2521 atgttggcgc gcgataacgg cgcgcgcttg agaacaccgc ggcacacaac tgtccgctgc
     2581 gcttttagca accgcttagg tctttaagtt ctcttggaaa tataactaac cgcgctccgg
     2641 acctggcggc ttagatcggt tcgcagggcg aacaactctt cggcagtaca ctggtctcgg
     2701 cggcaggcta acgcccgatc gatgggctcg agatcaagtc caggaggttg gacctggtgc
     2761 agatattcga cggcgcgatc gttggaatca accgccagct tgtcccaggt gaaaatcaag
     2821 cgggccgcgt agaggattgc gcgcacatag ggcttgcggt cttttgcctc gagccggctt
     2881 aacctctgga gctcaggaat acgcggctgc caactttttt cgatactttc acgcaatgat
     2941 tgatgaatct caaccttgat cggccgggga aagtccgctt tgtgctcgcc caataacggc
     3001 accccatgat ccagtagatc caaccgatct attaccggca aacggcccca gctgaactcg
     3061 ggattgcccc aaaacaccga gagctttttg cctaaatccg ggtgcaaact cttggcttga
     3121 gttaccagcg ttgggaattc ggatggcggc tctttgcaat tgagcaagag tcccacgtcg
     3181 atatcgctgt aaattccgct gaaaccgcca tgggcgaggc tgcccaactt gtatacttct
     3241 accaaggagg gcccgagttc agagcggaag tgctcggcga cgcgcacaac atatccggtg
     3301 atttcgttca tcgggcttgt gggatccaaa tccatacgac aaaaacacac ggctacgaac
     3361 cggcttcgct atcttgatct ggaatgttct ttatctcttc taaccaaacc gttgcttcgc
     3421 tatcgctggg cgcgcgataa tcgccgcgcg gcgacaacga gccacctgag ccgacctttg
     3481 gtgcattggg tatgcaactc cgcttgaact ggctgagctg aaagaagcgc caggcaaaaa
     3541 ccgaaagcca gtgcttaatt tcgccgatgg tgtactggtt gcgcttaggc tccggaacgt
     3601 cgggccagtg cccctgaacc cgatctctcc aggcgcaata cgacaaaaag gcgatcttcg
     3661 agggcgcgta gccaaagcga atggtgtaat agagagtgaa atcctgaagt tcatacggcc
     3721 ctatcacatc ttccgttttc tgggcaaacg gctctccatc atcttcggcc ggaataagct
     3781 ccgggctgaa gtcggtctcg agaattgccc gcagaacccg actcgattgt tcaccgagtt
     3841 ggtttgtctc agccacccag cgaatcagaa actgcatcag agttttcggg acgctggcgt
     3901 taatggcgta atgggacata tgatctccca ccccgtaggt ggaccaaccc agcgccagct
     3961 cactcaaatc actggtgcca agcaccaatg cttgatgaag attagcgagg cggaaaagat
     4021 ggctggtgcg ctctcccgcc tgaacgtttt cgaaggtaac atcgaaaacc ggttgcccgg
     4081 cggcataagg atggccgata tctcgcagca tctgctcaca gctcggccga atgtccaatt
     4141 cgtgggattc acaaccgata gcagccataa gtgcattggc attgtccaag gtatgctggc
     4201 tggtggcgaa gccgggcatg gtaaaggcca gaatgttaga acgaggcagt ttcagcacat
     4261 ccattgatcg cgccgccacc acgagcgcgt gagttgaatc aagccctccg gatatgccta
     4321 ttgcgatttt ggagattttg gaacttttga gccgcttgac caatccttgc acttgaatgt
     4381 tgtacgcctc atagcagcgc aggtcgcgca ccgctgggtc cgacggaacg taggggaatc
     4441 gatcaatctt tcgcctgcat aacagacggc ccattttcgg cgtctcaagg ctaaacgtaa
     4501 ctttgcgaaa gcggcgaagc tcatcgcggt gtagctgcat gctctggccg aaggtgttct
     4561 gacgcatgcg ctcgagcgcc agccgctcta gatcgagttc ggccagcacc agctgggaat
     4621 cgtaacggaa ccgttcggac tcgccgacaa gaacgccgct gtcatagacc attgcatggc
     4681 catcccatgc taggtcggtg gtcgattcgc cggtccctgc cgcagtgtag aggtaagcgg
     4741 cgatacaacg agccgactgg ttggagacca gattgtgccg atactcgtcc ttggcaatcg
     4801 ttatattcga ggctgagaga tttaaaatca ccgtggcgcc ggctaatgct gcataagacg
     4861 aaggtgggat cggcacccaa agatcttcac aaatttccac gcagaacgta aactcgcgct
     4921 gcctgtcggc ctggaatagc aagtgcgccc caaacggaat gccggtgtgg tcgaagatct
     4981 caacttcggt actgcaagct gcagctgccg gagcaaactg tcggtgctcg tagtactccc
     5041 ggtaattggg caggaacgtt ttgggaatca ctccaagaat cttgccgcgc gagaggacaa
     5101 ccgcgcagtt aaacaaaagg tggtcgattc gcagcggcat gccgactacg gctattagct
     5161 tcaaagcttc ggatgctttc aagatcattc gcagcgcatc caacgagcca tccagcaggg
     5221 cctgctggtg aaacaagtcg tcgcacgagt aggccgagag cccaagttcc ggaaacagcg
     5281 ccagaattgc cttgtcacgc tccgcctttt ccatgagctc aatggtgcgg ctaccgttaa
     5341 acgctgggtc cgcgacttgt agttccggaa taccgaccgc gacccgaatg agcccgtgat
     5401 tgtatagatt gaaaaattcg gcgttttgca tggctatcta gtctgggaat ggaagatgtc
     5461 tgatttttac ccaatgagca aacgaacgcc gtgatgcttt atttccgcaa gcttcaccgt
     5521 cttacgcttg gatcatagca gatgccgccg tgatcggatc ggcattgcat aaggcattct
     5581 gcactgggac agcagatcga tccgaggtcc aaatcggact taagtcgtca ctcagtcacg
     5641 gatatcctgc aaaaaccatt cggtcggtaa cgcacggccg accttctttt gtttggccaa
     5701 ttcgacgacc ttgccggcgc aggcggcgaa ttgaagtcct tgggtgccgg cgttgatgaa
     5761 aaaagtcact tgctcgttgc tggttcggcc gggcaccttt ccggcccgaa catccatgaa
     5821 gtacttgaag taatcgcctt tgtagttatc gacagttgga ttggggatac ggctgcgctc
     5881 ttcgggctgt ccggcaatgt agccgacgtt gccatgcagg cggatcatgc cttcatccat
     5941 agcttcgatc gtgttcttgc cgagcttaac tgacaaatca cacttctgga tcacctttgg
     6001 gccaacctcg caggctcgca cgcacgtgat atgcgctcct cgtttcagcc actccggctc
     6061 gtcgaacacc gttcgggtcg agtccgtgca ggttgcaacg atatcggcgc cgcgcacaac
     6121 cttttctggt gcgtctacgg caaccacttc caaatccaat ttgccggtca tttcccgagc
     6181 aaagctttct cgattggctt ttgtcgggct gtagactctg actgttttca gtttacgcac
     6241 ttcataaaac gcttccaggt agctgcgtgc catgccgccc gaaccgaaga tgccgacatc
     6301 ggaagcatcc tcgcgagcca aatacttgac ccccagtccg gcgcagccgc ccacccgcat
     6361 atgctgcatc aaaccgtcat tgatgatcgc cagcggctcg ccattgcgca cgctaaaaac
     6421 cataaccagc ccgcaccagg tacccggctg catgcaatat ttttcttcgg tgtcgccgtt
     6481 gggccagtaa accacatcgg acttcatccg tatggcgaac acaccgatct ttcggctggc
     6541 gccttccatc gttccccaac gccaatagcc gtccggccga tcacacggca tccaaagatc
     6601 caggcgcggg cgataaaccg cgtcacctat aagcaaatcg tcgtacgcgt cttccagagc
     6661 cgcaagacag gctttcatgt cgaagagctt ttccacttcc tggttgttga tgagtaacat
     6721 agcgtcctcc tgctgattgc ttatatcaat gtcagagatt tgaaaagaca aaccagaatt
     6781 gatccagcat aaagaacgca cagtgctggt gaacggtgaa gccgcaattg caccctgcaa
     6841 gaatatttcc aactactcct taaagtccca aatacttctt agggaggatt gacgagggtt
     6901 cagccggaag gttaaacatt agaggttaat tctggaaata tgggcggcgc tcgggttcag
     6961 caaaaccata ggtgagagca agggaaagct ctcctaacca tcttgaggag ggaagaacga
     7021 tgacgttaag aagcttcaga gaagtttttt tcggcgcaac tcgcgactcc ttcaactctg
     7081 tgagaacaat accgatggtc atggtcgccg ccatcatgtt tgccggaata gcgggggcac
     7141 aaaactccga gacggatgcg ctagaacagc aaccattttt ttcagccagg ctatccgggt
     7201 tcaacgaggt tatttttgtt gccgggcaag caggaccgcc gttggtacct cctgctctcc
     7261 ggggcgcgat ctcaaccccc gccaacgggc gattcaccgc gatcctcgac tccccggact
     7321 tgatccagta cgagcttagc tacgagggac tggtagcgcc agtgactcag gcgcacatcc
     7381 atttcggcca gcgccataca gttggcggca tcatcgtctg gctgtgccaa accgcgggaa
     7441 cccaggcgcc ggaggctgtg cgttcttcaa cgccgctctg tccctcagaa ggaaccgtga
     7501 gtggaacgat tactcctgca caggttcttg cccaagacgc ccagggattt gcagccggcg
     7561 actttgatga gctggttcgc gcgatccgca atcgagcggc ttacgccaac gtccacactg
     7621 aaaaatttac cgctggggaa atccgtggtc agattaggag caatcggaat agagacaatt
     7681 agccgtgaca tagattctaa aatcggagcg ccgagccaca tgaaaagccc cgcccaacgg
     7741 ggcttttcag tttcaaacac tcgcagacgc cgcagtcctg ttgcgaaatc cactacatgg
     7801 gtaggaaccc gctccatttt gttaacaggg attagacttc cgaaagaacg gtacatgtta
     7861 atgactggcg cctgttgctc aagaaaccga aatctgcagt gacttcttgc tgaatacgag
     7921 ccaaaggaaa atcttagcat ggtgattcgc atcgcatcgg ccgttctgac aatggctgga
     7981 ctactcgctt tgatctcggg aattctgttc tggaccggga ccgctgtgaa tctcatagcg
     8041 ttgcacatgc ttctcggatt cttggccgta ggcgcgcttt gggtcattgg attgacgcag
     8101 gcctttgctc agcatggcag ttggattatc gcggcgtgcg cgctgatcgt tggcgcggcg
     8161 atgatcgtcc tcggcatgat gcagtcatcg cttttgcccg gcgagtatca ctggatcata
     8221 caagccattc acttggcact cgggctcctg acaatcggca taggccacat ggccgctgcg
     8281 cgctatcgga aagcctccgt tggcgaacag tcaacgcccc aacgtcattg aagagaatgc
     8341 gttacaggct gcttaaaaag actcagaagc gaggcgcgcg acaaatcgac gagcggaggc
     8401 gtactcgctc agtacgttga cgcgaggcga ttgaagcgca acgaagctta tgaggctttt
     8461 tcagcagtct gctagatcgg ccactgctct aatcgatacg cctgttccca aaacatcaac
     8521 tcatatcggc tgctgaggat aaagtgttcg acgagaagct ttttttctcc aggttttgcc
     8581 tcggcgccca gtatgtccat taattggatt tgctcccgga ccggcttcca gaactcttcg
     8641 gaggcataaa tttcgatcca ttcccgatag atcttcggct tgcctggcgc tccctttttt
     8701 aggcgccggc cggcttcccc atagatccag tagcaaggaa ggacagccgc gacgatctcg
     8761 ctcagactgc cgctacgagc gacggactgg agatgaccgc tataggcctg cgtgatcggt
     8821 cccttctgca ccgcatctaa ctgttcgcgc gataatccca gagtcttacc gaaactcgcg
     8881 tgaaaactgc gctcgacttc gaccgccccc agtgcatggc gggcaaacag ttcgagcgtt
     8941 gccagatcgg ccgacttggc cgcgccgaga cagagaactt gggcgaagtc gagcaaatag
     9001 acgtaatcct gcaggatgaa ataggtgaac cgatccatcg gcaaggttcc ggcgtgcaat
     9061 tcacgcaaaa acgggtgact ctcaatccgt tgccagattc gctcgctgcg acgtctt
//
LOCUS       DASUTG010000352         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_796370, whole genome shotgun sequence.
ACCESSION   DASUTG010000352 DASUTG010000000
VERSION     DASUTG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796370"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1815)
                     /locus_tag="VF089_08050"
     CDS             complement(40..1815)
                     /locus_tag="VF089_08050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6173952.1"
                     /translation="MPLSFLAAAACASPSKSPEGAVQTAQPAKPASSARPADATAAYH
                     FMLGYQAELSQNSEQALKEYEAALKADPNALSVKARLASLHFSLGDMPNALRYAEEVA
                     DGRAEDGRMLTHMAGILAGGGKGDKALSVLDRAVELDPESGDAYFSKGLLLLNLKRPA
                     EAEQAMRAGIARSPDNAVGHYQLGRVLLEAGKVEEATTSFERAITANYSFEPAYLALA
                     AIYESRHEKERAVGVLKRYLQQVNSRNRDIRHQLVRLYIDAKDYSGARKELEAMIAED
                     PSDLDAQLRMALIHGEQKEYPQAIERLTEILKVRPAELKVRDYLGYVYEESKDTQKAL
                     ETYTFNVQLEPTFFEGHLHLGVLYYRLKKFPEATEHLGRAIALNPKQPESHIVQGLAY
                     LQQDQYDKSAEVFQEGIRHNPKSADLHFNLGTAYDKLDRFDDVIREMETAIQLDPHHA
                     DALNYLGYSYADRGIKIDQAISLTKQAVALKPENGYYVDSLGWAFFKSGLLTEALAEL
                     KRAAALVGDDPVIYEHLGDIYAKQQRLSDAREAWLHALELDPSNHKLMDRFREQGMGD
                     PAQEERIQQAKRRVAGQKPSLPIAQ"
     gene            1940..2077
                     /locus_tag="VF089_08055"
     CDS             1940..2077
                     /locus_tag="VF089_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173953.1"
                     /translation="MAGSNDFTIVEAFELCESVMEKEFLFPYRTIAMLADENVGYTFA
                     L"
BASE COUNT          409 a          578 c          586 g          512 t
ORIGIN      
        1 ggattgaaga tgatcgcaag aaaatgatcg gttcgttcgt tattgcgcga tcggtagaga
       61 gggtttctgg ccagcgactc tgcgcttggc ctgctggatt cgttcttctt gtgcaggatc
      121 acccatgcct tgctcacgga accggtccat caacttgtga ttgcttggat ccagttccag
      181 ggcgtggagc caggcttcgc gggcatcgga aaggcgttgc tgtttggcgt aaatgtcgcc
      241 gagatgctca tagatcaccg ggtcgtctcc caccagtgcg gccgctcgct tgagttctgc
      301 aagggcttcg gtcaataacc ccgatttgaa aaatgcccat ccgaggctgt ccacatagta
      361 gccgttctca ggtttgagcg cgaccgcctg tttcgtcagg gaaatcgcct ggtcgatttt
      421 gattcctcga tctgcgtagc tgtatccgag atagttgagc gcatcggcat gatgcggatc
      481 aagctgaatg gcggtttcca tctctcgtat gacatcatcg aagcggtcta gtttgtcata
      541 ggccgttcca agattaaaat gcaaatcggc actcttggga ttatgacgga tgccttcttg
      601 aaacacttcg gcagatttgt cgtattgatc ttgctgcagg taagctaatc cttgcacgat
      661 gtgggattcc ggctgtttgg ggttcagcgc gatcgcccga cccaggtgtt ccgtcgcttc
      721 agggaacttc ttgagccggt agtacaagac ccctaaatgc aaatgccctt cgaaaaacgt
      781 gggctcgagc tgcacattga atgtataggt ctcgagcgct ttttgcgtgt cttttgattc
      841 ctcgtagaca tagccgagat aatctctcac tttcagctct gccggacgga ccttgagaat
      901 ttctgtcaac cgctcgatcg cttgaggata ttccttctgt tcgccgtgga tgagggccat
      961 tcgaagctgc gcatccagat cggacggatc ttcggcaatc atcgcttcaa gttcttttct
     1021 ggcacccgaa tagtccttgg cgtcgatata gagccgcacc agctggtggc ggatatcccg
     1081 gttgcgggag ttgacttgtt ggaggtatct cttgagcact ccaaccgcgc gctctttttc
     1141 atggcgcgac tcgtagatgg cagccagtgc cagatatgcc ggctcgaacg agtaattggc
     1201 ggtgatggcc cgctcgaagc tggtggtcgc ctcttccacc ttccccgcct ccagcaggac
     1261 ccgtcccagc tgatagtgcc cgacggcgtt atcgggcgat ctggcgatcc cggcccgcat
     1321 cgcctgctcc gcttccgccg ggcgcttaag attgaggagc aagaggcctt tcgagaaata
     1381 cgcgtctccc gattcgggat cgagttccac tgcgcggtcc aatacgctca acgccttgtc
     1441 acctttaccg cctccggcaa gaatgcccgc catgtgcgtc aacatgcgcc cgtcttcggc
     1501 ccggccgtcg gccacctcct ccgcataacg caaggcattt ggcatgtcgc ccagcgaaaa
     1561 atgcaggctg gccagccgtg ctttcacgga cagagcattg gggtcggctt tcaatgccgc
     1621 ctcatactcc ttgagggctt gttcggaatt ttgcgacagc tcggcttgat agccgagcat
     1681 gaagtggtag gccgcagtcg cgtcagctgg acgcgctgac gatgccggct tggccggttg
     1741 ggcggtttgg accgcccctt cgggtgattt cgacggcgaa gcgcaggctg cagccgccag
     1801 gaaggagagc ggcatcaacg cgcacagaca ccgaagcgac caggctgccg aggtgctata
     1861 caattccatc cgtctcatcg gacgatcaac agcaatcctg agaggactcg caaacactca
     1921 tcgattatac gaagcggtca tggcagggtc aaacgacttc acgattgtcg aggctttcga
     1981 actttgcgaa tcggtcatgg aaaaagagtt cctttttccc tatcggacca ttgcgatgct
     2041 tgctgacgag aatgtcggct atacctttgc gctctgagtt ctgat
//
LOCUS       DASUTG010000353         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1092771, whole genome shotgun sequence.
ACCESSION   DASUTG010000353 DASUTG010000000
VERSION     DASUTG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092771"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1174
                     /locus_tag="VF089_08060"
     CDS             230..1174
                     /locus_tag="VF089_08060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6173954.1"
                     /translation="MQFGLHLPASTETVTSQDLVRFARRAEELGFYCITVADHIIVPK
                     NISVPYPYTADGKYPGAGYHLETLSTIGFLAGATSRIRFVTSVLIAPYRNPVITAKML
                     ASLDVLSGGRIIVGLGVGWMKEEFENLQAPPYQERGRVTDEYIRAFRELWTHDAPSFQ
                     GEYCSFADIIFLPKPVQKSIPIWIGGHSRQALRRAGQLGDGWHPIGGVPTIPLEPEDL
                     KRNLETLRGFAQAAGRDPGAIRVAFKGSLYDREKQIVPGKRRRFLGANDQIIGDIRDY
                     RDAGVDTLIFDVRRPSAAETLERMEWLAKEVLGKIENA"
     gene            complement(1395..1688)
                     /locus_tag="VF089_08065"
     CDS             complement(1395..1688)
                     /locus_tag="VF089_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173955.1"
                     /translation="MKKLCYWGVLIGWVLWIRTQSPTSDSWNALPGFGSRQLCAINAK
                     EKLAVWRQFKDAVISDNTVTFTENNTTMTYICLSDDDDPRRQQKPLAPEQPVS"
     gene            1836..2858
                     /locus_tag="VF089_08070"
     CDS             1836..2858
                     /locus_tag="VF089_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173956.1"
                     /translation="MAHNFEVIDADGHITEEDNQLKQYMDARYRDRAATLTPRDSWDR
                     SIGGTLGTRARDARSWLDAMDRGGVSTAVLYPTNGLSVGWIREPDVAVAYSKAWNDFV
                     SEEFQKVSPRLKAVALVPFQDVPEAVKELRRAVTQLKLPGLMLPAVGLRLPLGHESFW
                     PIYEEAERLDCMVGVHATVRGPHYFAADMFDQFIEVHTLSHSFAQMMQCTSMMFRGVF
                     DQFPKLRVAFMEAGCSWAPYWFGRMNEEWEKRGAVEAPKCKKKPTEYFKEGRIYFHAE
                     DYEPLIGATTEVISHNVIYYASDYPHWDTEFPENIDHLARRKDLNDEAKKWLLAESAK
                     KLYNLG"
BASE COUNT          676 a          766 c          843 g          675 t
ORIGIN      
        1 atctgcaatg atatggattt tcaaactatg agtttattta cccgagcttg ctgtctcgcg
       61 ggtggttgat agctccgcta aagtccgcca ttcccgcatg gttttagcgg gaatccaggg
      121 ggatttggac tggacccccg atttaaaatt cggggttgac gcttgggaag gaaatatcgc
      181 gagatcttct atccgcagct tgctgcatca ttgttcataa gagaaaccta tgcagtttgg
      241 actccatctg ccggcttcaa ccgagaccgt gacttcgcaa gacctggtaa ggtttgcccg
      301 gcgcgccgag gagctggggt tttactgtat tacggttgcc gatcacatta tcgtacccaa
      361 gaatatttca gtgccttatc cctacacagc agacggcaaa tacccgggcg ccgggtatca
      421 tctcgagaca ttaagcacta tcggctttct cgccggggca accagccgca tacgtttcgt
      481 taccagcgtc ctgatcgcgc cttatcgcaa cccggtgatt accgctaaga tgctcgcctc
      541 gctcgacgtt ctatccggcg gcaggatcat cgtcggactg ggtgtcggct ggatgaagga
      601 agaattcgaa aatctacagg cgccgccgta ccaggagcgt gggcgggtga ccgatgaata
      661 catccgcgct tttagagagc tatggacgca tgatgctcct tcgtttcaag gcgaatattg
      721 cagctttgcc gatatcattt ttctgcccaa accggtgcag aagtctatac cgatctggat
      781 aggcggtcac agcaggcaag ctttgagacg cgcaggacaa ctgggcgacg gctggcatcc
      841 catcggcggg gttccaacga ttcctctcga acccgaagac ctaaagcgga atctagagac
      901 tctgcgcggc tttgctcaag ccgccggtcg agatcccggt gccattcgcg tggctttcaa
      961 aggttcgttg tacgatcgtg aaaaacagat cgttccgggc aagaggcgaa gatttctcgg
     1021 cgctaatgat caaattatcg gcgacatccg agactatcgt gatgccggtg tcgatacctt
     1081 gatctttgac gtgcgtcgtc ccagcgccgc cgagaccctc gagcggatgg aatggctggc
     1141 caaggaagtg ctcggtaaga tagagaacgc gtgatcattt cggtgaacgt gacagattgc
     1201 ttctgagctc cgcagcgcgc tgctctcaaa gctctgaggg tagcccgagt cctcgtgatc
     1261 gtgtggcgca aacgtgattg agcaattccg aacgattacg agcacgcggg cacgaacacg
     1321 aatagggcca gtcggaatgt tgcgagcact gagtcaagtc gaagtgtcga aggacaaaac
     1381 ttctaagcag ccggctaact gaccggctgc tcgggtgcga gcggtttctg ttgacggcgc
     1441 ggatcgtcgt catcgctcag gcaaatatag gtcatggtag tgttgttctc cgtgaatgta
     1501 accgtattgt cgctgatcac ggcatctttg aactgccgcc acacagccaa cttttcctta
     1561 gcattaatgg cgcacagctg gcggcttcca aagcccggta aggcattcca actatcgctc
     1621 gtggggctct gagtgcgtat ccacaatacc caaccgatca gaactcccca gtaacagagt
     1681 ttcttcatag gctcttctgc tcctttaacc cgccggcgaa ggcgcgccga cagaatacct
     1741 cgaatcgctt atttggtaca ccgttctctt gacacacaag acggttgagg cgtagacggt
     1801 ttgaaggaac ccaaaaagaa gggggattag tcaatatggc gcacaatttt gaggtcattg
     1861 atgctgatgg ccacattact gaagaggata accagctcaa acagtatatg gacgcgcgtt
     1921 atcgcgatcg ggccgcgacc ctgacgccgc gcgacagctg ggatcgttcg atcggcggaa
     1981 cattagggac tcgcgcgaga gatgccagga gctggcttga tgcaatggat cggggcggtg
     2041 tttcgacggc ggtgctttac ccgaccaatg gtctcagcgt cggttggatc cgcgagcccg
     2101 atgtggcggt ggcttattcc aaggcgtgga acgacttcgt ttcggaagag tttcagaagg
     2161 taagcccaag actcaaggcg gttgcactgg tccctttcca ggacgtgccc gaagcggtca
     2221 aagagctgcg gcgtgccgta acgcaactca agctgcctgg tctgatgctg ccggccgtag
     2281 gcctcaggct gccgctgggg catgagagtt tctggcctat ctatgaagaa gcggagaggc
     2341 tcgattgcat ggtcggggtt cacgccaccg tacgcggacc gcattatttt gccgccgaca
     2401 tgttcgatca gtttatcgaa gttcacacat tgtcccattc cttcgcccag atgatgcagt
     2461 gcacgagtat gatgttccgc ggtgtctttg atcaattccc taaactccgg gtcgccttca
     2521 tggaggccgg ttgtagctgg gcgccctact ggtttgggcg aatgaatgag gagtgggaaa
     2581 agcggggtgc ggtggaagct cctaaatgca aaaagaaacc gacggaatat ttcaaggaag
     2641 gccggattta cttccatgcc gaggactatg agcctttgat cggcgccacc accgaagtga
     2701 tatcgcacaa cgttatctat tatgcttcgg actacccgca ctgggacact gagtttcctg
     2761 aaaacatcga tcaccttgcc agacgaaaag atctcaacga tgaggcgaag aaatggctgc
     2821 tcgctgagag cgcgaaaaaa ctctacaacc tcggctgaga tcaacctatg gcaacaagac
     2881 gtaaggtttg tcttaccggt ccgacgcggc tcaaagtggc cgaagaaatt ttgcagggcg
     2941 ccggttgtga actggtgctg
//
LOCUS       DASUTG010000354         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2131878, whole genome shotgun sequence.
ACCESSION   DASUTG010000354 DASUTG010000000
VERSION     DASUTG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2131878"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF089_08075"
     CDS             <1..216
                     /locus_tag="VF089_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6173957.1"
                     /translation="SSLVSQLLMEMCRKELPGRQLKSFDFKSARQIYDTGKFTIAGTP
                     AKDGREAELWALDSDGRVAMTATAKFE"
     gene            complement(316..1356)
                     /locus_tag="VF089_08080"
     CDS             complement(316..1356)
                     /locus_tag="VF089_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6173958.1"
                     /translation="MEPKNKPIADLGWQLDEWLRGQSDYVNADQFAGILRTLLKLAQD
                     DAERGDLKILNRTLQELRHAFRIFAPYRHLRKVSIFGSTRVQESDPYYDLARQIGEEL
                     ALADFMVITGAGPGIMQAGHQGAGREKSFGVNIRLPSIQDANPFIRNDPKLMNFHFFF
                     TRKLMFVKEADAVVIFPGGFGTHDELLESITLAQTGKTQLVPIILMDLPEGTYWARWV
                     EFLREDVMSRGYINEREMTFFRIHTSAVAAVDEINKFYRNFHSYRFVKHELVIRVNHP
                     PSLNLIDRLNRDFKDIVIGEVRQTEPLSAESNDANTLHLHRLLMRFNREDYARLRQMI
                     DIINDDKSTSQG"
     gene            1640..2659
                     /locus_tag="VF089_08085"
     CDS             1640..2659
                     /locus_tag="VF089_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6173959.1"
                     /translation="MNGRVWQCLIAMLLLLPGSLYADDFYKGKTIRVIVGGSAGGGFD
                     IYTRAMARHMGKHIPGNPSLVVENMTGAGTFIAAKYIHSSAKPDGLTFGMFNGALILG
                     RVLGMKGVDIDMRELEFLGVPVQDSTVCALRKESGVTNVDQWFASKTPMKLGGLSPGN
                     STSDVARMVGATLGLPIQLVEGYKGTNEIRLAADSGELHGACWAWETLKVAWQNAIPA
                     GDVNVVLQVTAKKLPDLPNVPMALDLAKSDEARLLLKAGAIDPAAIVRVYVTTPRIPK
                     DRLQILRKAFAATLTDPEFVAEAKKSNLDINPLTGEEVKKTVDGLFKLSPAVVSKLAD
                     ILAVK"
     gene            2698..3708
                     /locus_tag="VF089_08090"
     CDS             2698..3708
                     /locus_tag="VF089_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173960.1"
                     /translation="MIRRSISMLFMLLMALLLAGQIAYSAQTELKPEKNPLEVAIAAT
                     GPLYLPILLANEAGYFSKRGLTVNISTLSATASAQALLSGQVDIYQGGTATIHANVGG
                     ADLIYIAASVDRSTLMLFGQKGLTSFEDLRGKSVATTSVGAFGEIAMRKTAKEHGMEV
                     GKDLKLLYHKGPPDALSTFLLGNAEGVIITPPQTEMARSKGFPVIIDFYEKGLKIIGP
                     GTGVARTFVKKHPNTLKIFLMGYLDGVKRSLDDPAYARKILSQTSKITDPKLLEDSYK
                     EGVKVWNKDMTVDPQAIKVVLEQSTIPKVAELDPNRFFDNTLIREVNRDYASKLFPGE
                     VK"
BASE COUNT          921 a         1052 c         1076 g          843 t
ORIGIN      
        1 agctctctgg tctcacagct gctgatggag atgtgtcgca aagagctgcc ggggcgacaa
       61 cttaaatcct tcgacttcaa gagcgcccgg cagatttacg ataccgggaa gtttaccatt
      121 gccggcactc cggcaaagga cggccgtgag gcggaattgt gggcgttaga ctcggatggc
      181 cgagttgcta tgaccgccac ggcaaagttc gagtagtatc aaagcttcag ggcgaacatt
      241 ccgccgccgt gctaatgatg cgcggcggag ctgaatcggc gatgaggata aagatcctcg
      301 caagccttgt atcgatcatc cctgagacgt tgacttgtca tcattgatga tgtcgatcat
      361 ctggcgcaag cgggcgtagt cttcgcgatt gaagcgcatc aggagccggt gcaaatgaag
      421 cgtattggcg tcgttgcttt cggcggagag aggctcggtc tgccggactt cgccgatgac
      481 gatatctttg aagtcgcggt tgagccggtc tatcaagttg agtgaaggcg ggtgattgac
      541 gcgaatcacc agctcgtgtt taacgaagcg gtaagaatga aagttgcggt agaacttgtt
      601 gatttcgtca accgccgcca cagcgctggt gtgaattcgg aagaaggtca tctctcgctc
      661 gttgatatat ccacggctca tcacgtcctc acgcagaaat tctacccaac gggcccagta
      721 ggtgccctcc ggcaaatcca tcaggatgat cggcacgagc tgagttttgc cggtttgtgc
      781 cagggtgatc gattccaaaa gctcgtcatg cgtgccgaag ccgccgggaa agatcacaac
      841 cgcgtcggct tctttgacga acatgagctt gcgcgtaaag aagaagtgaa agttcatcag
      901 ctttggatcg tttcgaatga acgggttggc atcttgaatc gacggcagcc ggatattaac
      961 gccaaagctc ttttctcgcc ccgccccctg gtgtcccgct tgcataatgc ccgggccggc
     1021 cccggtgatc accatgaaat ccgctagcgc cagctcttcg ccgatctgac gcgccagatc
     1081 atagtaggga tcgctttcct gcacccgggt ggagccaaaa atgctgacct tgcgcaagtg
     1141 ccggtatggc gcgaaaattc tgaaggcgtg acgcagctct tgcagtgtcc ggtttaaaat
     1201 cttcagatct ccgcgttcag cgtcgtcctg cgccagcttg aggagggtac gcagtattcc
     1261 tgcgaactgg tccgcgttga catagtcgct ctgcccgcgc aaccactcat ccaattgcca
     1321 gcccagatcc gctataggtt tatttttcgg ctccaattat gccaccttca gctcgttcac
     1381 tttgttctcg tccatgcttc agccggacct gcgccagccg ctagagcacc cgcataaaga
     1441 cattataaac aacgaagcac gtatcggata gagaaaggaa tgttctacga caccgcgcaa
     1501 cgcacctaca cctcgtgaat cactgatcgc ggcaagcaca ggaagacaca ttacgtcgaa
     1561 aacgaccgag gcttgagggc agaaagagat tgacaagagc gctattttcc cgatacagcc
     1621 ggacgtggag gactacgtta tgaacggtag ggtttggcaa tgtttgattg cgatgcttct
     1681 cttgcttcct ggctcgcttt acgctgatga cttttataaa ggcaagacca ttcgggtcat
     1741 cgtcggcggc agcgccggcg gcggcttcga tatttacacc cgggcgatgg cgcggcacat
     1801 gggtaaacac attcccggga atccttcatt ggtcgttgag aatatgacgg gcgcgggaac
     1861 cttcatcgcg gccaaatata ttcacagcag cgcgaagccc gacgggctca ctttcggcat
     1921 gttcaacggc gccttgatct tgggacgcgt gctgggaatg aagggcgtcg atatcgacat
     1981 gagagaactc gaatttcttg gcgtgccggt gcaggacagc acggtttgcg ccttgcgtaa
     2041 ggagagcgga gttaccaatg tcgatcaatg gtttgcctcg aagacgccaa tgaagctggg
     2101 cggcttgagc ccgggcaata gcaccagcga tgtggcgcgg atggtcggcg cgactctcgg
     2161 gctgccgatt caactcgtcg agggttacaa aggaaccaac gaaattcgtt tagcggcaga
     2221 ctccggcgaa ttgcatggcg cttgttgggc ctgggagacg ctgaaggtcg cctggcaaaa
     2281 cgccattccg gcaggagacg tcaacgtcgt cctgcaggtc acggcgaaaa aactgccgga
     2341 cttgccaaat gtgccgatgg cgctcgatct ggcaaagagc gatgaagcaa ggctgttgct
     2401 taaagccggc gctatcgatc cagcggcgat cgtgcgtgtc tatgtcacca caccacgcat
     2461 cccgaaagat cgtctgcaga tacttcgcaa agcttttgct gcgacactga ccgatccgga
     2521 attcgtcgcc gaagctaaga aatccaatct cgacatcaac ccgttgaccg gcgaggaggt
     2581 caaaaaaacc gtcgacggct tgttcaagct tagtcccgcc gtagtctcca agcttgctga
     2641 tattcttgcc gtcaagtaac cgagaagaat tgtcgtagac ggagagccga gagaatcgtg
     2701 attcgaagat cgatatcgat gttgttcatg ctcctgatgg cgctgttatt agcaggtcaa
     2761 atcgcatata gcgcgcagac cgagctgaaa ccggaaaaga atccgcttga agtagccatt
     2821 gcggccacgg ggccgctcta tctgccgatt ctactagcca atgaggcggg atatttcagc
     2881 aaacgtggct tgaccgtcaa catcagcacg ttgagcgcca ctgcatcggc gcaagcactc
     2941 ctctccggcc aggtggatat ataccaaggc ggcacagcca cgatccacgc caacgtgggc
     3001 ggcgcggatt tgatctacat tgcggcgtcc gtcgatcgca gcacgctcat gttgttcggc
     3061 cagaagggtc tgacatcgtt tgaagatctc cgcggcaaat ccgtcgccac cacttcggtc
     3121 ggagctttcg gcgagattgc catgcgcaaa accgccaagg agcacggcat ggaagtcggc
     3181 aaagacctca agcttctcta ccataagggg ccgccggacg cgctgtcgac ttttctcctg
     3241 ggtaatgccg agggcgtcat catcaccccg ccgcaaaccg aaatggctcg cagcaaaggc
     3301 tttccagtga ttatcgattt ctatgaaaag ggtctcaaga tcatcggtcc cggcacggga
     3361 gtagcgcgaa ctttcgtaaa aaaacatccg aacactttga aaatatttct gatgggatac
     3421 ttggatggcg tgaagcgaag cctcgacgat ccggcgtacg cgcggaaaat tcttagccag
     3481 accagcaaaa tcaccgaccc caaactgctc gaggacagct acaaggaagg cgtcaaggtg
     3541 tggaacaaag atatgactgt cgatccgcag gcgatcaagg tcgtgctcga gcagtcgacc
     3601 atcccgaaag ttgcagagct cgacccaaat cgcttcttcg acaacacgct catccgcgag
     3661 gtcaaccgcg actacgcttc caaactattt cctggtgaag tgaagtaacc gttttcatcc
     3721 gggcatggcg ctcttactgc cagaatccat cgtcattctc tgacgacttc atccgtccat
     3781 cgatctcagc ggacagcgta tcccccacgc ggactgccaa gcagtttgct atagtgaatt
     3841 gatgaatcat ctaaatgaaa aaatcgtcac cttcttagat ccagaggcga tt
//
LOCUS       DASUTG010000355         6802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_499730, whole genome shotgun sequence.
ACCESSION   DASUTG010000355 DASUTG010000000
VERSION     DASUTG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499730"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF089_08095"
     CDS             <1..303
                     /locus_tag="VF089_08095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6173961.1"
                     /translation="FGNVVLEALSSGLPVLVSSEVGAAEVLSGALLQGIVDKPDDPVE
                     LEAKMVALLEHAAEPELIKEATTLAARFSWHNHFRQLEDLLFAVVSSRCDVRANVA"
     gene            293..1132
                     /locus_tag="VF089_08100"
     CDS             293..1132
                     /locus_tag="VF089_08100"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6173962.1"
                     /translation="MLPDFVLLRHRATAVWIDAEFNKRAVNELVWDPDRFFSDERCVV
                     VKDQKKIKVARVAVEVSGVLYRIFIKRYNTFSWRARLLSIFTRSGAFRSLEGAAILLN
                     AGFATARPLAALESRRCRVVTKSFYLSEEIAGGKTADAYWREDLTKAQPQRLRRRRQF
                     ISGLALTFQALHRQGVYHNDLKDANIIVVPEKNAERFYLLDLEGTRRYSDLNLRRKVK
                     NIVQLNHTLGRLLRRTEKMYFLKVYLHSSFASKRMKRRWVTAVLEQSRRRDLKSRRAR
                     KPR"
     gene            1304..2218
                     /locus_tag="VF089_08105"
     CDS             1304..2218
                     /locus_tag="VF089_08105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6173963.1"
                     /translation="MSRKVSILIPTHNRAASLKRLLESLEASACLEETQLEIIVVNNH
                     STDATSQVLAEVLAEDRRFPILVLEEPKHGKSWALNLGLGHVTGSLVLLLDDDVSVDK
                     FCVAEHVAAHKDGLFDAVQGKVLAGLDLEGQPANMTRLREYNIPTVDHGDAPCEVRGF
                     TGTNISIKHEVFEKAGLFDTRLGPGASGFSEDTEYSARIRENGFKIGYAPKAIIYHEL
                     NPNRYGSSYNRTVQYRKGVSRSIYRKDSLIFRVLPDLIGNCFRYLAYTAMGRRQKAYR
                     TQGRIAKNYGYIAGKVQKRMLQHKRSRS"
     gene            2428..3237
                     /locus_tag="VF089_08110"
     CDS             2428..3237
                     /locus_tag="VF089_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6173964.1"
                     /translation="MDDRQLNAAAKPSVSAIVVCYNEEDNIGRCLSSLRWCDEIVVVD
                     SFSTDRTVEICRQFTSIIIEREWAGYREQKAFAHSKATKDWVLMIDSDEEVTPELKDE
                     ILEQLAVAGTLCVGFLLPRLVFYLDRWWRRGGWYPDYDVRLFRRELATWGGTDPHDKI
                     IVDGPVRRLRNPLHHFSYRNIDDHIQRINRFTSISSRELRTQGECWRLSDALLRPAFR
                     FCRSYFLKRGFMEGFAGFHVAVTAAMYVFLKYAKLWELELKEKKEVGRKRS"
     gene            3218..4321
                     /locus_tag="VF089_08115"
     CDS             3218..4321
                     /locus_tag="VF089_08115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6173965.1"
                     /translation="MGGSGHSRLLKILHIDPEKNWGGGEAQVVGLLENLSRRGHRNDL
                     ATPADGQLFQRSIPRVRKIPLSVRNDLDVRGVPALRRLIRQERYDIVHLHTKRAHALS
                     LWLPRGEPRPKYIVTRRMDYPEPKNCYTRYLYNRRVDGVVAISRSVLDCLSEAGVNRR
                     SIRLIYSGIDPMPFTGLLSDDQRAADSAVVAMLAVLEERKGHRFLLDAARVLKDRGRK
                     IRYVLAGDGSLKTELQRLVQTLGLGEEVSFVGFVKDVPKFLSSIDVLVLPSLYEGLGV
                     AALEGMAAGKPVIATRVGGLAESVVDGETGFLVPPEDGAALANAIEKLVADPSMARAM
                     GRKGAARVLDNFTIEQMASQNEAYYYALLDCMP"
     gene            4346..5338
                     /gene="rfaE1"
                     /locus_tag="VF089_08120"
     CDS             4346..5338
                     /gene="rfaE1"
                     /locus_tag="VF089_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX6173966.1"
                     /translation="MFGSERLTEILNKFRKVNLLVVGDLMLDRFIWGEVERLSPEAPV
                     PVLRVCSESSRLGGAANVVHNVRSLGAQVTACGVVGADGAGKTLIDDLQRIGASTSGV
                     FADREVHSIQKTRIIARPSHQQIVRLDRENHTAIRAATLERICRFILNKASRFDAILV
                     SDYGKGVIHANLLNCLASLAANKELRLVIDPKKENYGRYQRASLITPNKDEASAASGI
                     TIRDDQTLATAGQRLLQMWRAKAVLITRGPEGMTLFRPRQAAQHFPTQPREVFDVTGA
                     GDTVVAVCALALAAGARYEEAAVLANYAAALVGDEVGTVAVPLGRLHRIIKDLP"
     gene            5335..6201
                     /locus_tag="VF089_08125"
     CDS             5335..6201
                     /locus_tag="VF089_08125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX6173967.1"
                     /translation="MRSMTGYGEASHQNKYAKITVQVRSLNHRHLDLQLRVPREYLGF
                     EEEIRKSLRETVSRGRIELFINRTSAPGHAREVELDEELLGKYLKAIQRAKKKFYLKG
                     ELGVSIFSSVPDLLRVRDTEVNLRMEKQALFTAVRSALQKLEESRLREGRHLRIDMQS
                     QLRHLRSTASDLEKHASQFAPSLMKVSAAGRSDEAATKAGNEAVDAMSAVLKGDINEE
                     VVRLKTHTAALGTILREPGAVGKKIDFILQEIQRELNTISAKVPVLAVVQLVLQGKER
                     VEKIREQTQNVE"
     gene            6198..>6802
                     /gene="gmk"
                     /locus_tag="VF089_08130"
     CDS             6198..>6802
                     /gene="gmk"
                     /locus_tag="VF089_08130"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX6173968.1"
                     /translation="MKTNRFINKRKGIVFILSAPSGTGKTTVISEVLERFPDISLSVS
                     YTTRAPRAGEARGRDYHFISHAKFLAMTAKGEFAESASVHDFFYGTPARTLDRAMASG
                     RDLFLDIDVQGARQIKRKYPEAVSIFLLPPSWSELKRRLGGRATDDRAIIRRRLANAR
                     REIQDVIQYDYYIVNSDIQEAVGTLDAVILAERQKISRVTA"
BASE COUNT         1590 a         1686 c         1959 g         1567 t
ORIGIN      
        1 tttggcaacg tagtcctgga ggcgctttct tcgggcctgc cggtcttagt gagtagcgaa
       61 gtcggagcag cggaggtttt gtcgggcgct ctgcttcagg gcattgtcga caagcctgac
      121 gacccagtag aattggaagc caaaatggtc gcgctcctgg agcatgccgc tgagccggag
      181 cttatcaaag aggccacgac cttagcggct cggttttcat ggcacaatca cttccggcaa
      241 ttagaagatc tcttgtttgc agtcgtttcc tccaggtgtg acgtgcgtgc aaatgttgcc
      301 tgatttcgtc ttgctccggc atcgagccac agcggtctgg atcgatgcag aatttaataa
      361 gcgggcggtc aatgagctag tctgggatcc cgatcggttt ttttctgatg aaagatgcgt
      421 cgttgtcaaa gaccagaaga aaatcaaggt tgcgcgcgtt gcagtcgaag tctctggagt
      481 gctttaccgg atattcatca aacgttacaa cacgttttcc tggcgcgctc ggttactatc
      541 gattttcacc cgatccggag ccttccgatc cctggaaggc gcggcgattt tactaaacgc
      601 cggttttgca accgccaggc cgcttgcggc gctggaatca cgtcgctgcc gggtggtaac
      661 caaaagcttc tatctcagcg aggaaatagc cggtggaaag accgccgacg catactggcg
      721 tgaagattta acgaaagctc aacctcaaag acttcggcgt cgtcggcagt ttataagcgg
      781 gttagctctg acgttccagg cgttgcatcg gcagggtgtt tatcacaatg atctaaagga
      841 cgctaatatc attgtggttc ctgagaagaa tgctgagcgt ttttacctcc ttgatctgga
      901 agggacgcgc cgctattccg atctcaatct ccggcgcaag gtcaagaata tcgtccagtt
      961 gaaccatact ctgggccgtc ttctccgtcg taccgagaaa atgtattttc ttaaagttta
     1021 tctacactct agttttgcca gcaagcgaat gaagcgccgc tgggttaccg cagtgcttga
     1081 gcagagcagg cggcgcgatc tgaagtcacg acgcgcgcgc aaacctcgct aggtcatgct
     1141 cggtcgagct ttgttagttg gcaccacccc agcggctatc gttaactggg agctatggcc
     1201 cgagtggaca ctcgcttgat gtcagttcat cttccatcca gcagttggca gtgatgggtg
     1261 tcgtgtagca agcacggtta tcttatgaat cggtgaggtg atcttgtccc gtaaagtcag
     1321 catcttgatt cctacgcata atcgcgcagc cagtttgaag cggctcttgg aaagcttgga
     1381 agcatcggca tgtctcgagg aaactcagct agagattatt gtggtcaaca atcactcgac
     1441 agatgccacg tcgcaggttc tagcggaggt tctagctgag gatagaagat ttccaatcct
     1501 ggtgttggag gagcctaaac acggtaaatc ttgggcattg aacctaggtt taggacatgt
     1561 aaccgggagt ctggtcttgc tacttgacga cgacgtttcg gtcgataagt tttgcgttgc
     1621 cgagcatgtt gctgctcata aggatgggct ttttgacgcg gttcaaggga aggttttagc
     1681 tgggcttgac ctcgaaggac aaccagccaa tatgacgcgg ctgagagagt ataatattcc
     1741 aacagtcgat catggtgacg cgccatgcga ggttcgcggc tttaccggga cgaatatttc
     1801 aatcaagcat gaggtatttg aaaaggccgg cttgtttgat actcggctgg ggcctggcgc
     1861 ttccggattt agcgaggaca ctgagtactc tgcgcggatt cgtgagaacg ggttcaaaat
     1921 aggttatgcg cctaaggcga taatttatca cgagctcaac ccgaaccgat acggcagttc
     1981 ctacaacagg actgttcaat atcgcaaagg ggtcagccga agcatctatc ggaaagattc
     2041 cctgatattt cgcgtcttgc ctgatctcat cggcaattgc tttcgttacc tggcttacac
     2101 cgccatggga cggcgacaaa aagcctacag gacccaagga cgaattgcga aaaattacgg
     2161 ttatatagcc gggaaggtcc agaagcggat gctgcaacat aaaaggagtc gatcataact
     2221 gcgcgtcaga aatctctcgc ctcgatgatg aaagctactt tggtctagtt tatccgtctc
     2281 atgaagcatc cggaaagtat tggatcacct aatccgatca ggcgctgttt cattagggca
     2341 ctagcgctat tataactagt ttgttgagga ttttagagcg ccgacttctc gcgccgttag
     2401 tcagcggtca ccttatcttc tgaagccatg gatgacagac agctcaacgc cgcggcgaaa
     2461 cccagcgtgt cggcaatcgt tgtttgctat aacgaagaag acaatattgg ccgttgtttg
     2521 agtagcttgc gatggtgcga tgaaattgtc gtagtagatt cgttcagtac cgatcgtacg
     2581 gtagagatct gccggcagtt cacgagtatc atcatcgagc gcgaatgggc gggatatcga
     2641 gagcagaagg ccttcgccca ttcgaaagcg accaaagatt gggtgcttat gatcgactcc
     2701 gacgaagagg tgacgccgga gctgaaagat gagattcttg agcaattagc agtcgccggc
     2761 acactttgcg taggattcct gctgccccgg ttggtgtttt atctagaccg ctggtggcgg
     2821 cgcggcgggt ggtatcctga ttatgacgtc cgattgtttc gccgggagct cgcgacgtgg
     2881 ggcgggaccg atccccatga caaaatcatc gtcgacggcc cggtgcgccg cctgcgaaac
     2941 ccgctgcatc atttctcgta ccgcaatatc gatgaccata tccaacgaat caatcggttt
     3001 acttccattt catcgagaga gctccgcacg caaggtgaat gctggcgcct gagcgatgcc
     3061 ttgttgcggc cggcgttcag attctgtcga tcttatttct taaaaagggg tttcatggaa
     3121 ggctttgcgg ggtttcatgt ggccgtcact gccgccatgt acgtcttctt gaagtatgct
     3181 aagctctggg aacttgagct aaaggagaag aaggaagttg ggcggaagcg gtcatagccg
     3241 cctgctaaaa atcctgcaca tcgatcctga aaaaaattgg ggaggtgggg aggcgcaggt
     3301 agtcggtctt ctggagaatc tatcccggcg gggccaccgc aatgatctcg cgacgcctgc
     3361 tgacggtcaa ttatttcagc gctctatccc acgcgtccga aagattcctc tcagcgttcg
     3421 caacgatctg gatgtaagag gagttcccgc gctccgacgc ctaattcggc aggaaagata
     3481 cgacatcgtt catctgcaca caaagagagc gcatgcgctc tctttgtggc tgcctcgagg
     3541 cgagcccagg ccgaagtaca ttgtgacccg acgcatggac taccccgagc cgaaaaattg
     3601 ttacactcgg tacctttata atcgccgggt ggatggggtt gtcgccatct ctcgttcagt
     3661 tctcgattgt ttgtcagagg cgggcgttaa tcgtcgttcc atccgtttga tttatagcgg
     3721 catcgaccca atgccgttca cagggctgct aagcgacgac cagcgcgccg ccgactcggc
     3781 agtcgtagca atgcttgcgg ttttggaaga gcgaaaagga catcgttttt tgttggatgc
     3841 ggcgcgagtg cttaaagatc gcggtcgaaa aattcgctat gtactcgccg gtgacgggtc
     3901 actcaaaact gaacttcaaa ggctggtgca gacattagga ttgggagagg aagtgtcctt
     3961 cgtgggtttc gttaaagatg ttccgaagtt tctctcctca atcgacgttc tggtgctgcc
     4021 ctctttgtat gaaggcttag gtgtggcggc gctcgaggga atggcggcgg gaaagccggt
     4081 gatagcaact cgggttggcg gcctggccga atcggttgtc gatggcgaga cggggtttct
     4141 agtgccgccc gaagatggcg ccgcgctggc gaatgcgatc gaaaagctag ttgccgatcc
     4201 gtccatggca cgagcaatgg gacggaaagg agcagcgcgg gttctggata attttaccat
     4261 cgagcagatg gcaagtcaga acgaagcgta ttactacgca ttgctcgatt gcatgccgta
     4321 aaggtcttcg gagagagact acttcatgtt tggcagcgag cgattaacgg agatattaaa
     4381 caagttccgc aaagtaaatt tgctcgtggt cggcgatctc atgctcgacc gcttcatttg
     4441 gggagaggtt gagaggcttt ctcccgaagc gccggtgccg gtgctgcggg tatgttcgga
     4501 aagttcgcgt ttgggtggcg ctgccaacgt ggtccacaac gtcagaagct tgggggcaca
     4561 ggtgaccgcg tgcggggtcg tcggcgcgga cggagcgggt aagacgttaa tcgatgacct
     4621 tcagcgcatc ggcgcatcaa cgagcggcgt attcgccgat cgagaagttc acagcattca
     4681 gaaaacgcgc atcatcgccc gtccaagcca tcagcaaatt gtccgccttg accgtgaaaa
     4741 tcacacggca atcagagctg cgactctcga gcggatctgc cggtttatcc tgaataaagc
     4801 gagccgcttc gacgcaatcc tggtgtcgga ttacggcaag ggagtcattc acgcaaactt
     4861 gttgaattgt cttgccagcc tcgccgcaaa taaagaactt cggctggtta tcgaccccaa
     4921 gaaggaaaac tatgggcgct atcaacgcgc gtctttgata acacccaaca aagacgaagc
     4981 cagcgctgcg tcagggataa ctattcgtga tgaccagacg ttggcgaccg cgggacagcg
     5041 cttgctacag atgtggcgcg caaaagcagt cctcatcacc cggggaccgg aaggaatgac
     5101 tctttttcgg ccgcgccaag ccgcgcaaca ttttccaact caaccacgcg aggttttcga
     5161 cgtcaccggc gccggcgaca cggtagtggc agtttgcgca ttggcgctgg ctgccggagc
     5221 ccgttacgag gaggcggcgg tgctggccaa ttacgccgcc gccttggtgg gtgatgaagt
     5281 cggcacagtc gcagtgcctc tgggcaggct ccatcgcatc ataaaggacc tgccatgaga
     5341 agcatgacgg gctatggcga agcgtcgcat cagaacaagt atgccaagat tacggtccag
     5401 gtcagaagct tgaatcacag acatctcgat cttcagctta gagtgcctcg cgaatatctc
     5461 ggcttcgaag aggagatacg taagagtttg cgggaaaccg tcagccgcgg acggatcgag
     5521 cttttcatca atcgaacatc cgcgcccggt catgcgcgag aggttgaact ggacgaagag
     5581 ctgcttggca aatatctgaa ggcgatccag cgcgctaaaa aaaagtttta cctgaaaggt
     5641 gaactcggag tttcgatctt ctccagcgtt cccgatctac tgcgcgtgcg cgataccgag
     5701 gtcaatctgc gaatggaaaa acaagctctg ttcaccgcag tccgatcggc gcttcaaaag
     5761 ctcgaagaat cgcggctccg agaaggacgt catctgagga tcgatatgca gtcccagctg
     5821 cggcacttga ggagcactgc cagcgacctg gaaaagcatg ccagtcaatt tgctccttcg
     5881 ctaatgaaag tttcggcggc aggaaggagt gacgaagcag cgacgaaggc cggcaatgaa
     5941 gccgtagatg ccatgagtgc ggtgcttaaa ggcgacatca acgaagaggt ggtgcggctt
     6001 aaaacgcata cagctgcatt aggcaccatt ctccgggaac cgggagccgt cggaaaaaag
     6061 atcgatttta ttctccagga gattcagcgg gagctcaata ccatcagcgc gaaagttcct
     6121 gtccttgccg tggtgcagtt ggtgctgcag ggaaaggaaa gagtcgaaaa aatccgcgag
     6181 cagacgcaga acgtcgaatg aaaacgaatc ggttcatcaa taaacgcaaa ggtattgtct
     6241 tcattctgtc ggcgccttca gggacgggta aaaccaccgt gatcagcgaa gtgttggagc
     6301 ggttcccgga tatctccctc tcggtctcct acaccactcg agcgccccgc gcgggagaag
     6361 cgcgtgggcg ggattatcat tttatcagcc acgcgaagtt tttggctatg acagcaaaag
     6421 gcgaattcgc cgagtccgcc agcgttcatg atttctttta tggcaccccg gcgcggacct
     6481 tagatcgggc aatggccagt ggcagggatc tgtttctcga cattgacgtg cagggcgccc
     6541 gacagatcaa aagaaaatac cctgaagcag tgtcgatatt cctgctgccg ccctcgtgga
     6601 gcgaattgaa gcgacgtttg ggcggccgtg cgacagatga cagggcgatc attcgccgcc
     6661 gcttggccaa tgcccggcgt gaaatccaag atgtaatcca gtacgattac tatatagtta
     6721 atagcgatat ccaagaagcc gtcgggactt tggacgcggt tattctggcc gaaaggcaaa
     6781 agatttcgcg ggttacagct tg
//
LOCUS       DASUTG010000356         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_352613, whole genome shotgun sequence.
ACCESSION   DASUTG010000356 DASUTG010000000
VERSION     DASUTG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352613"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..993)
                     /locus_tag="VF089_08135"
     CDS             complement(79..993)
                     /locus_tag="VF089_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6173969.1"
                     /translation="MGQLLQGRVALVAGATRGAGRGIAVELGAAGATVYCTGRTTRTQ
                     ASELNRPETIEETAELVQRAGGRGIAVQVDHLDPAQVQTLVARIEREQGRLDILVNDV
                     WGADFLIEWNVPVWKHSLENGLRMLRLAIDTHIITSHFALPLLIKNPGGLVVEMTDGT
                     AEYNNANYRLSLFYDLAKTSVIRMASAQAKELRPHQCTAVALTPGWLRSEQMLDNYGV
                     SEVNWRDAIAKQPHFVITETPRYVGRAVAHLAGDPEVERWSGQSLSSGQLAQIYGFTD
                     VDGSQPDAWRYVVEVQDAGKPADATGYR"
     gene            complement(1084..1482)
                     /locus_tag="VF089_08140"
     CDS             complement(1084..1482)
                     /locus_tag="VF089_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6173970.1"
                     /translation="MNEKVSQSEYLVISRGQWDRELSPPEIQKAIDQFYLWLGRLVDE
                     GKMKTGQRLASDGKTVRKNVVTDGPFGEAKEVIGGYWFIVAGSLDEAAEIAVGNPCLN
                     CGLFFEIRPIDPERASAFTVTTETPIGNRK"
     gene            complement(1534..2064)
                     /locus_tag="VF089_08145"
     CDS             complement(1534..2064)
                     /locus_tag="VF089_08145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6173971.1"
                     /translation="MEVIKLRSATPADLKLLQDWDEQAHVIASDPNSDWGWEVELARN
                     PDWREQLIAELNGRPIGFIQIIDPEREESHYWGDAAANLRAVDIWIGSETDLGKGYGT
                     KMMALALARCFDNPSVSAVLVDPLASNTRSHRFYERIGFRFLERRRFGDDESFVYRLD
                     RADYMQAADILQAARE"
     gene            complement(2346..>2916)
                     /locus_tag="VF089_08150"
     CDS             complement(2346..>2916)
                     /locus_tag="VF089_08150"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6173972.1"
                     /translation="SSFLGRVTDPEGAAGEPIREAGFRAIMFTDMANSTEITHGLGDA
                     KAQSVLAKHHEIIRQALLAHEGREVDRAGDGFLTCFASVSQAVACAVTVQRGFQTYNL
                     SGAGPVKIQVRIGLGAGEPLTDGDTLFGSTVNLTARICAAADPGQILAARVVRELCSG
                     KSFTFKPYGEVNLKGFPDPVELYEVDWAG"
BASE COUNT          633 a          883 c          752 g          648 t
ORIGIN      
        1 gcaggtttcg tggcgcagcc tctcgccgga ttactacatc atcaacgaaa aagttctctg
       61 accccatttc tgtttgcttt acctgtagcc ggtagcatcc gctggtttgc ccgcatcctg
      121 gacctcaacg acgtaacgcc aagcatccgg ctgcgaaccg tcgacatcgg tgaagccata
      181 gatctgggcg agctgcccgc tcgatagcga ttgaccgctc cagcgctcaa cctcaggatc
      241 tccggcgagg tgtgcgaccg cacgcccgac gtatcgcgga gtttcggtga tgacgaagtg
      301 cggttgcttg gcaattgcgt cacgccagtt tacttcgctg acgccgtagt tatcgagcat
      361 ctgctcggag cggagccaac cgggagtcag ggcgacggca gtgcactggt gcggccgaag
      421 ctctttcgct tgcgccgagg ccatgcgtat aacggaagtc ttggcgagat cgtaaaacag
      481 cgacaaccgg taattggcat tattgtattc ggcagtgcca tcggtcattt ccacaaccag
      541 tccgccggga ttcttgatga gcagcggcag cgcaaagtgg ctggtgatga tatgcgtgtc
      601 gatagcaagc cgcagcatgc gcaaaccgtt ttccaacgag tgcttccaca ccggcacgtt
      661 ccactcgatc aagaagtccg ctccccatac atcgttgacc agaatatcca ggcggccttg
      721 ctcacgctcg atccgcgcca ccagcgtttg cacttgcgcc gggtccaaat gatcgacttg
      781 cacggcgata ccgcggccgc cggcgcgctg caccagctcg gcggtctctt caatcgtttc
      841 cggtcggtta agctccgaag cttgcgtgcg cgtcgtacgc ccggtgcagt acacggtggc
      901 tccagccgct cccagttcga cagcaatgcc ccggcccgcg ccgcgcgttg cccctgcaac
      961 caatgccact ctaccttgga gtagttgacc catcctcaac ttttttcagt cgttgactca
     1021 aaggtttctc acaacatctt cacacaccga tgttgcacgt ctataattaa ggagatcttc
     1081 gactcacttt ctatttccaa tcggcgtctc ggtcgtgact gtaaaagcgc tggcccgctc
     1141 cggatcgatg ggccggatct cgaaaaacaa gccgcagttt aggcatgggt ttcccacggc
     1201 aatctcggcc gcttcgtcga ggctgccggc gacgatgaac cagtagccgc cgattacctc
     1261 cttggcttct ccaaaagggc catccgtcac aacatttttt cttactgttt ttccgtcact
     1321 cgcgagtcgt tgaccggttt tcatttttcc ttcatcaacg agcctgccca accacagata
     1381 gaattggtca attgccttct gtatctccgg cggcgagagt tctctgtccc attgtccgcg
     1441 cgaaatgacc aggtattcag attgtgaaac tttttcgttc atggcttttc ctccaggtgg
     1501 gtttcatgcg ggtgctgcag cgtaacgttg cctctactca cgcgccgctt gtaagatgtc
     1561 cgcggcttgc atgtagtcgg cacggtcgag gcgatagacg aaggattcat catcgccgaa
     1621 gcgacgccgt tcgagaaatc ggaatccgat gcgctcataa aaacgatgag atcgagtatt
     1681 gctcgcgagg ggatcaacaa gcactgctga aacggaggga ttgtcaaaac aacgagcaag
     1741 agccagtgcc atcattttgg ttccgtagcc tttacccaga tcggtttcgc ttccgatcca
     1801 gatgtcgacg gcgcgaaggt tcgcagcggc atcgccccag taatgacttt cctcgcgttc
     1861 cggatcgatt atctggataa atccaatcgg ccggccattc agctcagcaa tcaactgctc
     1921 ccgccagtcc gggttacgag caagctcaac ttcccagccc caatcactgt tcggatctga
     1981 ggctatgaca tgcgcctgtt catcccagtc ctgcagcaac ttcaggtcag cgggagtggc
     2041 ggaacgcaat ttgataactt ccataaactg ttgctcgctg acacatcagc tggaaccaaa
     2101 tgggctcacg cggaagtccg ctataggacg ctctattaag cagctccaac cttcccttac
     2161 tcataacgat aagctcaagc aaattggtcg cctccatatt gattcatcgc ccatctcaaa
     2221 tttatggatc cggcggcaag ccaagaaaaa atcacagccg tgagcacaag ccaatcacga
     2281 cctgcaatct gcacagctat gatgagaaga actattgtga gcggcaccgc tcgccgctga
     2341 gcacactatc cggcccaatc aacttcgtaa agctcaaccg gatccgggaa gcctttgagg
     2401 ttaacctcac cgtacggctt gaaagtaaaa gatttgccgc tacacagctc gcgaacaaca
     2461 cgcgctgcca gaatctgacc tgggtcagca gctgcacaga tcctggccgt gagattgacc
     2521 gtcgagccga acagcgtgtc accatcggtc aacggctcgc cagccccgag accgatgcgc
     2581 acttgaattt tcaccggtcc tgcgccgctg aggttgtagg tctggaagcc tcgctgaacc
     2641 gtcaccgcgc aggcgacggc ctgggacacg gatgcaaagc acgtcagaaa accatctccg
     2701 gcccggtcga cttcgcgccc ctcgtgagcc agcaaagcct ggcgaatgat ctcgtggtgc
     2761 ttagccaaga cactctgcgc cttggcatcc ccgaggccgt gcgtaatctc ggtggaattc
     2821 gccatatcgg taaacatgat ggccctgaaa cccgcttctc ggatcggttc acctgctgcc
     2881 ccttcaggat ctgtaactcg gccgagaaag ctggag
//
LOCUS       DASUTG010000357         8904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1538393, whole genome shotgun sequence.
ACCESSION   DASUTG010000357 DASUTG010000000
VERSION     DASUTG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538393"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF089_08155"
     CDS             <1..215
                     /locus_tag="VF089_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173973.1"
                     /translation="KDVTDQWRAGFPSSIHLFVNSMPSSLALCAVSKARPMQLNVRTR
                     LMPSLSQHTTALNFQRLPLMVGNSIR"
     gene            complement(303..2552)
                     /locus_tag="VF089_08160"
     CDS             complement(303..2552)
                     /locus_tag="VF089_08160"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6173974.1"
                     /translation="MTYRTVGKALPRIEGEGKVTGQTKYAADLPFEGLLWAKILRSSI
                     PHGRIKSIDTSKAKALTGVRAVLTGADVKDVYVGTRVKDQPVLVHDKVRMTGDAIAAV
                     AADTKEIADQAISLIDVEYEELPYVEDPVEALKPSAPLIHEDRGKYKNAPKLPEGVSP
                     HNLQSYVLWKNGDIEAGFQKAARVFEHTFRTPLSHHGYIEPCACTVQVHDDGRVEVWA
                     SNKGPWGLREQMAEDFGLEKEKIKVHIVHVGGDFGAKASLVDVPVAYYLSKATKQPVK
                     LVFDYTDELLAGGHRHPAVLALRTGVDQDGHFTAIKATIHFSGGAYGSQKANPQVTVL
                     GGRRLASMYRVPAISCETFCSYTNQVPCTQTRTPGSPQVVFAFESQVDIIAKEMGIDA
                     LELRRRNILHDGDANPMGEKWSDILMGEVLERAVKTSGWKKNGAKKNHGWGMALYDRG
                     TPEGKASSALTLEADGRVNILTGVPDVGPGYYTVSQQMVCETLGLPPEKVGVDFKDTD
                     SLPFDPGTGGSKQTNTSGHAVNQSAHEVREKLVALAARELGCQPEEIRQEGGKLIAPN
                     KKSTTTQNMIELAVKENGGPVFHLTNFVPKDMPKVTGFAAQVAEVEVDPTTGRVRVLN
                     LTTAHDTGTVLNNLTLTGQIEGGIITGLGFALMEENPMIDGKIATLTMGESKLPCIAD
                     VPPLKTVLVESPTGPTPFGGKAIAENPNVPTAAAIANAVADACGARIFDLPLSPEKVF
                     WALHGKINN"
     gene            complement(2704..3813)
                     /locus_tag="VF089_08165"
     CDS             complement(2704..3813)
                     /locus_tag="VF089_08165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173975.1"
                     /translation="MKSGFKIMDSDMHLREPADLWDKYMEPEFRDQAPKILSSTARSS
                     AMVLLDGSILRGYQPTYRGGIFDATRIDMEIAEARAKKFDSESQLQAMDREGLDVAAL
                     YPSIGLGIMMREQMSPKLAAAVARAYNNWLYDFCQADPKRLKGVAMLPLHDPAEAVKE
                     AERAVGKLGFVGVFARPEPLHDLPWHSRYFDTLWSALEDLGVPLGFHSAAAAGELPQI
                     GDRFGDNLLLRHVVSHPMENMLAMADIIGGGVCDRHPKLKIAFLECYCGWVSFLLHRL
                     DNAMEKGRFPTAGKLKPSEYFQRQCWISTEHEKELPMIIKLVGDDRIVFSTDYPHGDS
                     DFPRAVEEFLEMDGIAKESQKKILWDNCARLYNLA"
     gene            3995..5104
                     /locus_tag="VF089_08170"
     CDS             3995..5104
                     /locus_tag="VF089_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6173976.1"
                     /translation="MFDFLFPTEPFGGQTLRLVAQAQQGGGDLFDIARTCRDIKPGDK
                     QSWEKGWLDLAQRTEARAREALGAGRKITARDYFFHANQYYRMSDVFLTYNENDRKAE
                     RFRKAQECFRAGAELYTPPIEVISVQCGGEKYDGYFCHPDRPSAGKWPALLFLGGADA
                     YAEEIYFGAKLMTERGWAVLLVDTPGRGSSIYLKSIGTRADYEIPGKACVDYLISRPE
                     VDSDRLALTGISMAGYYAPRVAAFDKRIKALVAWSGCYSILDDLYDFCHHLQPTVQRL
                     LGGVSHEEARKRLKDFTMAGIAQNITCPTLITHGDKDTLMSVEGAKRLFAELGTKDKT
                     LRIYNEKDGGGRVHCSHDYWAHNVPYMLDWLEERI"
     gene            5315..6820
                     /locus_tag="VF089_08175"
     CDS             5315..6820
                     /locus_tag="VF089_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6173977.1"
                     /translation="MASNGKTTQWDKETDVIVVGYGAAGGISAITAHDSGASVLLIEK
                     MPHPGGISILSGGGVAFAHNAEGAFQYLKRTCNGTTPDDILRKMAEEMVGMIDWVTDL
                     AKVSGTEPTKTETRGHGTYPFPGTNDIDSIKLKDFQAYSDFPWAKGLRGGARLFKVVY
                     DNVKQRGIEVMLSTPAKELITGNDGEILGLVAEHDGKPLRIRAKKGVILACGGYENDD
                     AMKLQYFEAQPVYPVYLGNTGDGIKMAQKAGAGLWHMWHFHGGYGFKFPELPFAIRHV
                     WAGPRNENRKMIWIAVDKFGKRFMDEYPPAPQDTGTRSLEFYDADIQDYPRIPCYLIF
                     DEEGRKLGPIGIPIVNDQRYDASWSEDNLAEINKGWIKKGNSLEDIAGQIDVNAKVLK
                     ATVERWNSLCENGQDNDHKRPPKTMMPIKNPPFYVVEAWPIVNNTQGGPEHDVAQRVL
                     DPMKKPIPRLYVAGEISSIYGHLYLEAGNITECFVAGKIAGQNAASEQNWE"
     gene            complement(6831..7523)
                     /locus_tag="VF089_08180"
     CDS             complement(6831..7523)
                     /locus_tag="VF089_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173978.1"
                     /translation="MVRYIQGMPSGTKHKDAPETIPVKVTVNGSKMNIDTPRNEAPIL
                     LPFPIFEPPGYLMPGKLELKLTGAVTGSFGADPNEFGRRHGVQHVELKVSGYDAVAFA
                     RLVAKTAYANAYVHDQLKRLKNKPELVQAMMKEPNTIRRFVGTVPEPYQRHPGLQHRL
                     AIHIMPEHRLLYSTVQLFASAGAPAYIVVLGTLKDDDSMSDVRLGDEKTNRDRAHPML
                     AVDANTFRRPCD"
     gene            complement(7999..8541)
                     /locus_tag="VF089_08185"
     CDS             complement(7999..8541)
                     /locus_tag="VF089_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173979.1"
                     /translation="MIGGKAHGPNRQYQVECRDVLTFRNPELTPWASDGIDVPFELPD
                     TCWTFDVALRDPAGALIVAECRRTIGAVKQEDLAAFAYKVEVLRKALDIPIGGVFIAK
                     KDHQIGAIKVAQFNGIQVAILEEGATPPGFNVTFLRYDAERESRCRDIVMHVPPGSYA
                     LTGFPAMLVHTKASGEIETK"
BASE COUNT         1983 a         2547 c         2314 g         2060 t
ORIGIN      
        1 gcaaagacgt cactgatcaa tggcgtgccg gctttccttc gtcaatccat cttttcgtga
       61 attctatgcc ctcaagcctg gctttatgtg ccgtttcaaa ggcgcgaccg atgcagctca
      121 acgttcgtac gcgcctgatg ccttcactct cccagcacac tacagcgcta aatttccagc
      181 gtctgccact aatggtgggt aattctatca gatgaacgtg atgtccatca tgttcgaatt
      241 ccacaattgc ctcgccatcg gtcttgaatc accttcgtaa tctcctctac ctacggtata
      301 cctcagttgt taatctttcc atgcaaagcc cagaatacct tttccgggga caggggcaga
      361 tcaaagatcc gggcgccaca ggcgtccgcc accgcgttgg cgatggccgc cgcggtcggc
      421 acattggggt tttcggcgat ggctttaccg ccaaatggcg tcggacccgt ggggctttcg
      481 accagaacgg tttttagcgg cggcacatcg gcaatgcatg gcagcttgga ttcgcccatt
      541 gtcaacgtcg cgatcttgcc atcgatcatc gggttctctt ccatcagcgc aaagcccagg
      601 ccggtaataa ttccgccttc gatctgcccg gtgagagtga gattattgag caccgtgccg
      661 gtatcgtgcg ccgtagtcaa atttagaacc ctcaccctgc cggtagtggg atcgacttcc
      721 acctcagcca cttgcgcggc gaagccggtg accttaggca tgtccttggg gacaaaattg
      781 gtcaggtgaa agactgggcc gccgttttct tttacagcca gctctatcat gttttgcgtt
      841 gtcgtcgact ttttgttggg cgcgatgagc ttgccgccct cctgccgtat ctcctccggc
      901 tgacagccta attcccgcgc cgccaaggcg actaatttct cgcgcacctc atgcgcagac
      961 tgattgaccg cgtgaccgga agtattcgtt tgcttgctgc cgccggtacc ggggtcgaaa
     1021 ggcaggctat ctgtgtcttt gaaatccacg ccaactttct ccggcggcaa cccgagcgtt
     1081 tcacaaacca tttgctggct gacggtatag tagccgggac cgacgtcggg cacgccggta
     1141 aggatattaa ccctgccatc ggcctccagc gtcaatgccg agctggcttt cccttccggc
     1201 gtaccgcgat catagagcgc catcccccag ccgtggtttt tcttcgctcc gtttttcttc
     1261 caaccggaag ttttgaccgc gcgttccagc acttcgccca tgagaatatc gctccacttc
     1321 tcacccatgg gattggcatc gccatcgtgc agaatattcc ttcggcgcag ttcgagagca
     1381 tcgatgccca tctccttggc aatgatgtcc acctgcgact cgaacgcgaa cacgacctgc
     1441 ggactgccgg gcgtgcgcgt ttgagtgcac ggaacctgat tggtatacga gcaaaaagtc
     1501 tcgcagctga tcgccggcac tcgatacatg ctcgcgaggc gccgtccgcc aagcacggtc
     1561 acctgaggat tggctttctg cgagccgtaa gcaccgccgc tgaagtggat agtcgctttg
     1621 atcgcggtga aatgtccgtc ctgatccact cccgttcgga gcgctagcac cgcgggatgg
     1681 cggtgaccgc cggcgagaag ctcgtctgtg tagtcgaaca ccaattttac cggctgcttc
     1741 gtcgccttgg acaggtaata agccactggc acgtctacca acgatgcctt agcgccgaag
     1801 tcaccgccga cgtgaacgat atggactttg attttctcct tctccaggcc gaagtcttcg
     1861 gccatctgtt cgcgtaatcc ccagggtccc ttgttcgacg cccagacttc gacccggccg
     1921 tcgtcgtgaa cctgcaccgt gcaggcacag ggctcaatat aaccgtgatg cgaaagcggc
     1981 gtgcggaagg tatgctcgaa aactcgtgcc gccttctgga aaccggcttc gatatcgccg
     2041 tttttccaca acacatagga ctgcaggttg tgcggcgaga caccttccgg caattttggc
     2101 gcgtttttat atttgccgcg gtcctcgtga atcagggggg cgcttggttt caatgcttcg
     2161 accggatctt cgacgtaggg cagctcttca tattcgacat cgatcaggct gatcgcctga
     2221 tccgcgattt cctttgtgtc cgcggcgacg gcggctatgg catcaccggt catccgaact
     2281 ttgtcgtgca ccaggaccgg ctgatccttg acccgggtgc cgacataaac atccttgaca
     2341 tctgcgccgg tcaataccgc gcgcaccccg gtcaacgcct ttgctttcga agtgtcgatg
     2401 ctcttgatcc gcccatgggg aatcgagctg cgtaaaatct tcgcccacaa caatccctca
     2461 aacggtaaat ctgcggcata ctttgtttgc ccggttactt tgccttcgcc ctcgatgcgg
     2521 ggcagtgctt taccaaccgt cctatacgtc acttttacct ccacgaatct tcactgatta
     2581 cgttttgaaa ccgttccgtt tagatccttc actccgtaca ggatgacaac ggagtcattc
     2641 tgagcacggc aaagaatccg gttgattttg tctcgaacag ctctgaagtt atccgctcta
     2701 gctttaggcc aggttataga gccgcgcgca gttgtcccac aaaattttct tctggctctc
     2761 tttcgctatt ccatccattt ccagaaattc ttctaccgcg cgcgggaagt cggaatcacc
     2821 gtgcgggtag tcggtggaga atacgattct gtcatcgccc acgagcttaa tgatcatcgg
     2881 caactccttc tcgtgctcgg ttgagatcca gcattgtcgt tgaaaatatt cgctgggttt
     2941 taacttgccg gcggtgggga agcgcccctt ctccatcgca ttgtcgagac ggtgcagcag
     3001 gaaggaaacc caaccgcaat aacattccag gaaggcgatc ttgagcttgg gatgccggtc
     3061 gcacacgccg ccgccaatga tgtccgccat cgccagcatg ttttccatcg gatgagagac
     3121 cacgtgccga agcaagagat tatcaccgaa acggtcgcca atttgcggca gctcgcctgc
     3181 cgccgccgcc gaatgaaacc ccaaggggac tccaaggtct tcgagcgccg accatagcgt
     3241 atcaaaataa cgggaatgcc acggcagatc gtgtaaaggc tcgggccgag cgaaaacccc
     3301 gacaaaaccg agcttgccga ctgcgcgttc tgcttctttg acggcttcgg cgggatcgtg
     3361 aagcggcagc atcgccacgc ctttgagccg cttgggatca gcctggcaaa aatcgtaaag
     3421 ccaattgtta tacgcgcgcg ccacggctgc ggcgagcttc ggactcattt gctcgcgcat
     3481 cataatgccc aagccgatcg acggatacag cgccgccaca tccaatcctt cccgatccat
     3541 tgcctgaagc tggctctcgc tatcgaattt tttcgcccgc gcctcggcaa tctccatgtc
     3601 gatacgtgtc gcatcgaaga tgcccccgcg gtaggtcggc tgataacccc gcagaatact
     3661 gccgtcgagc aaaaccatcg ccgagctgcg cgccgttgaa cttaaaatct tcggcgcttg
     3721 gtcgcgaaat tccggttcca tgtatttgtc ccagagatcg gccggttcgc gcaaatgcat
     3781 atccgaatcc attatcttga aacccgattt catccgaatc gctccctatc atcgtcgcgg
     3841 atgtaaacgc actcgattgc tcaaagttat catgatcacc ggcgactctg tcaactgccc
     3901 ggcgcgtaga ctttagaaat agatttgcag taagatcgac tcttgaggtc caagccgata
     3961 agccaaggcg gatttgacaa cgactgaggt gaatatgttc gatttcttat ttcccaccga
     4021 accttttggc ggccagacat tgagattggt ggcgcaggcg cagcaaggcg gcggtgatct
     4081 gttcgatatc gcgcggactt gccgagacat caagccggga gataaacaga gctgggaaaa
     4141 aggctggctc gatctggcac aaaggactga agcccgcgcc agagaagccc tgggggctgg
     4201 aagaaagatc accgcgcgag attatttttt tcacgccaat cagtattacc gcatgtccga
     4261 tgtgtttctc acctacaacg aaaatgatcg taaagcagaa cgcttccgca aagctcaaga
     4321 atgctttcgt gccggcgcag agctgtatac gcctcccatc gaagtcatct ccgttcaatg
     4381 cggcggagaa aagtatgacg gttacttctg tcaccctgac cgcccctccg ccggtaagtg
     4441 gcccgccctt ctgtttttag ggggcgccga tgcttatgcc gaagagatct acttcggcgc
     4501 aaagctgatg accgagcggg gttgggccgt actgctggtc gacaccccgg gacggggctc
     4561 ttcgatttac cttaaaagca tcggcacccg ggccgattac gaaatcccgg gcaaggcttg
     4621 tgtcgactac ctgatctctc gtccggaagt ggattccgat cggctcgctt taacgggcat
     4681 cagcatggcc ggttattacg cgcctcgtgt agccgcattc gacaagcgca tcaaggctct
     4741 ggtcgcttgg agcggctgtt acagcattct cgacgacctc tacgatttct gtcatcactt
     4801 gcaaccaacc gtgcagcggt tgctcggcgg agttagtcat gaagaggccc gtaagcgatt
     4861 gaaagatttc acgatggcgg gcatcgccca aaacatcacc tgccctactt tgattactca
     4921 tggcgacaag gatactttga tgtcggttga aggcgcgaaa cgactgttcg ccgagcttgg
     4981 cacgaaggac aaaactctcc ggatctacaa cgagaaggat ggcggcggca gggttcactg
     5041 cagccacgat tattgggcgc acaacgttcc atatatgctt gactggttgg aagaaagaat
     5101 ctgaggaatg gagtggtaga gtaatggaaa ttgcgttact cccactacca ccatagaacg
     5161 cacactgtcc gatagtttct atccggtttc ttccccctcg acgggggaag attaagatgg
     5221 gggtgccggt cgaaaatcaa aatcacccct tccttttatc cttccccgtc aaggggaagg
     5281 atatccatcg atgattgaga aggagttttc tggaatggca agcaacggga aaacaactca
     5341 gtgggacaaa gaaacagacg tcatcgtcgt cgggtacggc gctgcgggag gaatttcggc
     5401 aatcacggca cacgattccg gcgcaagcgt tttgttgatt gaaaaaatgc cgcaccccgg
     5461 gggcatttcg attctgtccg gcggcggcgt tgccttcgcc cataacgccg aaggcgcttt
     5521 tcaatactta aaacgtacct gcaacggcac cacgccggac gatattctgc gtaagatggc
     5581 tgaggagatg gtaggcatga tcgactgggt tactgatctc gcgaaagtca gcggaaccga
     5641 gccgaccaaa accgagaccc gcggccacgg cacctatcca tttcccggca ccaacgacat
     5701 tgattcgatc aagctcaagg acttccaagc atattctgat tttccctggg ccaaaggact
     5761 gcgtggcggg gcacggctgt tcaaagtcgt ctacgacaat gtcaagcagc gcgggatcga
     5821 ggtgatgcta tcgacgccgg ctaaagaatt gatcactggg aatgatggag agatacttgg
     5881 gctggttgcc gaacatgacg gtaagccgct tcggatcaga gccaagaaag gcgtcattct
     5941 agcctgtggc ggttatgaga acgatgatgc catgaagctt caatatttcg aagcgcagcc
     6001 ggtctatccc gtctatctcg gcaataccgg tgacggcatc aagatggctc agaaagccgg
     6061 cgccgggctc tggcatatgt ggcactttca cggcggctac ggttttaaat ttcccgagct
     6121 gccgttcgca attcgccatg tctgggccgg cccgcgcaac gagaaccgca aaatgatctg
     6181 gattgccgtc gacaagttcg gcaagcgttt catggacgaa tatccgccgg ccccgcagga
     6241 taccggcacc cgctcgttgg aattctacga cgccgatatt caagattatc cgcgcatccc
     6301 ctgctatctc atcttcgatg aggaaggcag gaagctcggg ccaatcggca tcccgatcgt
     6361 caacgaccaa cgctatgacg ccagctggag cgaggataat ctcgccgaga taaacaaagg
     6421 ctggatcaaa aaaggaaact cactggaaga catcgcggga caaatcgatg tcaacgcgaa
     6481 agtgctgaaa gccacggtag agcgctggaa ttcactctgc gaaaacggcc aagacaacga
     6541 tcacaaacgc ccgccaaaaa cgatgatgcc gatcaaaaat ccgcccttct acgttgtcga
     6601 agcttggccg atcgtgaata acactcaggg cgggcccgag cacgacgtcg cgcagcgtgt
     6661 cctcgatccg atgaaaaaac cgatcccgcg gctctatgtc gccggcgaga tcagctcgat
     6721 ctacggacat ctttatctag aagcaggaaa tattacggaa tgttttgtcg ccggaaaaat
     6781 cgccgggcag aacgcggcga gtgaacagaa ttgggagtga ctaagtgttg tcagtcgcac
     6841 gggcgccgga aggtgtttgc atcgactgca agcattgggt gggctctgtc acgattggtt
     6901 ttttcatcgc ctaacctcac atcgctcatc gaatcgtcgt cttttaacgt gccaagaacc
     6961 acgatataag ccggcgctcc tgcacttgcg aatagctgta ctgtcgagta aagcaaccta
     7021 tgctcaggca tgatgtggat tgctaatcgg tgctgcaggc cagggtgcct ctggtagggt
     7081 tctggaacag tccccacaaa acgacggatt gtgttgggct ccttcatcat cgcctgtaca
     7141 agctcgggct tgttcttcag tcgtttcaac tgatcgtgta cgtaggcgtt cgcataagca
     7201 gtttttgcga cgagtctcgc aaaggctact gcgtcgtaac cactcacctt cagctctacg
     7261 tgttgcacac catgtcttct gccaaattcg ttggggtccg cgccaaagct acccgtgaca
     7321 gcgccagtca atttcagctc caatttgccc ggcattaaat atcctggcgg ttcgaagatg
     7381 ggaaatggca aaagaatagg agcttcgttt ctaggagtgt caatattcat tttgctgcca
     7441 tttacggtga cttttactgg aatggtttcc ggagcatcct tgtgctttgt tccagaaggc
     7501 ataccttgga tgtagcggac catccgcatc ggtccacgta gaactgccgt ttcaaacgca
     7561 tgtgtaatct tgcggcatcc ctcacaagac ccgcgctgta tggtcgtcgt tccgccaagg
     7621 gagtacgcga gaacgtgctc gcgacttaac tgcaccttag atccacagta aatacacgcg
     7681 tctgctgcct ttaaccgcag ctttgagaag tctgcaattt cggttggatc agcgacctca
     7741 acatttggga cctctgactt cgtcgcggtt ttccagtgaa ccctgccgtc gcggattagc
     7801 tcgaccttta tcctattact catcgaaaag ccggctcaac ggccgattcc ggagaggaat
     7861 tccggggaca caatacaaat ttatcaccat gcatctttca gtggacgcta cgcttttcct
     7921 cttccgtact cacggtccat caactcttcg atcatctcca cgaaattgcg cgctccctgc
     7981 taggcgacgc gggcagcact atttggtttc gatttcgccg gaggctttgg tgtgaactag
     8041 catagcagga aagcccgtta gtgcgtacga accgggtggc acgtgcatga cgatatcgcg
     8101 gcagcgactt tcgcgttccg catcatagcg caagaacgta acgttgaaac ccggtggggt
     8161 tgcaccctct tctaaaattg ccacctgaat accgttgaat tgggccacct taatcgcacc
     8221 aatttggtga tctttcttag caatgaacac gccgccgata ggaatatcga gagccttacg
     8281 caacacctcg actttgtatg cgaacgccgc taggtcctct tgctttaccg caccaatagt
     8341 acgtctgcat tcagcaacaa tcagtgcgcc agccgggtcg cgcagtgcaa cgtcgaatgt
     8401 ccaacacgtg tcagggagtt cgaacggcac gtcaataccg tccgaagccc agggggtcaa
     8461 ctctggattt ctaaatgtaa gcacatctcg gcattcaacc tgatattgcc gatttggtcc
     8521 gtgcgccttt cctccaatca tgccgttcct ctatctccgt ctaactataa cgatactagc
     8581 gctcgggtcg cgccgaccct aacggcccga gatcagctgg taatgcagcc atcatcactt
     8641 ccgccagaac cgcacttcat cacggtcgtc caagccgcac ctgctaaaca cttccttcag
     8701 caccttagcg tcatcggggt agatcgcagc catattgagc catgtgtagc cagacggcac
     8761 gtccagagat tgccacggag aagattatta tggatgcaca tcctgggtaa accaatcgag
     8821 cggcacttcg cgccccaacc ctttttcctt ggcggcttca aacaccttgg ccccgaccgc
     8881 ggcaaactgc acaccgagac cgat
//
LOCUS       DASUTG010000358        10773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1836545, whole genome shotgun sequence.
ACCESSION   DASUTG010000358 DASUTG010000000
VERSION     DASUTG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836545"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..809
                     /locus_tag="VF089_08190"
     CDS             447..809
                     /locus_tag="VF089_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6173980.1"
                     /translation="MKKVLIADDYPDMRELLGRQIESLGFLPILAENGKDVLEKAIRE
                     KPRLIMLDMIMPIMDGWQAVRSLRANPETKDIPILAATALFHQSDLNTCIEQGCNGYI
                     NKPFTMMELKKKVAELIR"
     gene            complement(873..1742)
                     /locus_tag="VF089_08195"
     CDS             complement(873..1742)
                     /locus_tag="VF089_08195"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173981.1"
                     /translation="MPLLAAPSRRYGIATEKGLFTKHGLNVDLRYIAPATATQALLAK
                     NLDIINPGGEIVEAGLNGEPVVFIAGILNRAVMSIYAKPEINSMSDLKGKVLGVTVPG
                     ATTDFAARVLLQQSGLAPGKDVKLLYLRGMPEILAGINQGNAVAGIFTAPTTLKARAA
                     GLKELMNVTEKNIPMIHAALATTRDYLKTHRDPTQRFLRAYLEGIKIAITDAEFTKQM
                     IGKYTKTTDPKDLESSYQAFLPAWERLPIVPAAAVQTMLNFASHPGAKAAKPESLIDN
                     SVLTEIDKVRVRR"
     gene            complement(1996..2883)
                     /locus_tag="VF089_08200"
     CDS             complement(1996..2883)
                     /locus_tag="VF089_08200"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173982.1"
                     /translation="MSPAYSPIWVVQEKRLLKKHGLNGEVISISASPIALQALLAGEV
                     DVIVTSVTTLALSRLAGADIIMILGMVPTFLDHVISHPTITTLEQIRGKTGGVNRLGS
                     TSDLGLRLALRRKGIDPEKDVKIITAGDNPARLAAISRGIIQFSIMPEPFVREAEKLG
                     FKDLFDIQSIKIPFWWNAVLAKESAVKANRAALTKFSRAMMEAIHYIKTDKEGTKAIY
                     SKNLKLTDPEGLERAYQAYVSVFPENLLPTPDGVKTMLDDLAPRNPKAAAVDPKAFVD
                     MSLVQEIEATGFIKQLYKQ"
     gene            complement(3048..3857)
                     /locus_tag="VF089_08205"
     CDS             complement(3048..3857)
                     /locus_tag="VF089_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6173983.1"
                     /translation="MALRINDSEYYAIDAHSHLGRRKTPLGHGVASFLGDDLVRNLDE
                     AGLDCAVAFPLGASYTDYSEANQIMAEEMAKHPQRIISFCRINPNFGPQASADSLEHC
                     LGNLKLKGIKLHPEIEFFDPNEAELMEPIYAAARRYHVPIIFHTGMSSKASPGVIAEL
                     AAQYRDVPVILGHMGVSEYVKLAVAVARQNENIFLETSVVGWMPLLLEAFRGVGTSKI
                     LFGSDHPYNPLAMEVEKISKHVTRAAKLNQDDLKKVFSTNFLSLLKWEHKI"
     gene            4220..4909
                     /locus_tag="VF089_08210"
     CDS             4220..4909
                     /locus_tag="VF089_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6173984.1"
                     /translation="MGNVARLSADQRKQAIVDAVRDVFAEKGFDGTTTRELAQAAGVS
                     EALIYKHFPSKESLYAAMLDACAKGPTFAQFKRILALEPSTSTLVLMVYFTISHYVLQ
                     RPGDPNKAALNCLLVRSLLEDGALVRLTHKRFASAWVAKFEACVKEAVKTGDIGATPI
                     RRDLRVWFVHHIAFSLMLHLRPKVPAIDYNVSRDALVEQATWFALRGVGFAEEAIKRH
                     YNPKALSLLGD"
     gene            4955..5401
                     /locus_tag="VF089_08215"
     CDS             4955..5401
                     /locus_tag="VF089_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173985.1"
                     /translation="MEPKKMASKKTHLEEEQQEKGLGAEDRLTPLFQPDTLMSEEYFA
                     NYRRRIPLEPEKALLLAVLEDGVRTFQDNIFAEIGKKRALLDEAREWLFMDALEHVFS
                     FNSVCSSLGLDPGYIRRGLKRWEEHARAATRQKQRPSQGAPERLVA"
     gene            5451..6581
                     /locus_tag="VF089_08220"
     CDS             5451..6581
                     /locus_tag="VF089_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6173986.1"
                     /translation="MIRTIGLLLLVLSAGCDRQAPASLERPPAPVSVAAAVTQDVPIY
                     IDAVGKIVAREVVSIQPQVSGRITKLHFADGADVKAGDVLFTIDPRPYQAHLNQAEAN
                     VAQAQAALNLAKTNFARVQSISDPRAISRQDFDAKKNAVEVAEAELRQNRAAVETARL
                     NLEYCTIRSPIDGRAGQRLVDVGNVVTPGNSPLLVIQRLDPIYADFTIPEADLTEVQR
                     NMARRTLRAEVRLAEESTKAREGDLTFLDNSVQEGTGTVKLRATVGNKDRLLWPGRFA
                     RIRLILDTHRSAVLVPAEAPQLSAKGSFVYVINQDDSAELRPVTIGQRHGDLVVIAQG
                     VNEGERVVINGQLGVTPGGKVQIAAPGAKKDPVAASQSGAKS"
     gene            6578..9724
                     /locus_tag="VF089_08225"
     CDS             6578..9724
                     /locus_tag="VF089_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6173987.1"
                     /translation="MSFSELFIRRPVMTTVLTASVVLFGILSYLRLPVNDLPAVDYPV
                     IQVQANYPGASPDTVANNIATPLERQFMQINGLELVTSKSTQGHTSLTLQFALDKSVD
                     AAATDVQTAISQATGSLPVDLPSPPTYSKTNPNDQPIMYIALTSDSVTVGQLYDYAST
                     QVGQRISILPGVSRVNVFGAKSAVRIKADPSAMWARGISVEDLAEAIRNGTSTIGAGQ
                     FDGAGGTLLLRPQGQLDDAKEYGDLIVSSKNGSPVYLRDVAQVKNSVQDERIKMRFWV
                     RGYDPPAATVIVAVNRKAGANAVEVAQSVRQLLPLISTELPGSVRVTPIYDRSQTIIH
                     SILEVELTLAIAFVLVVLVIFVFLGRATDTLIPAVALPLSLLITFVVMDLLGFSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMEHGQKALEATIASAREISFTIISMTISLAAV
                     FIPLVFMPGLVGRIFREFAVTIVVAIMASGLVSLTLTPLMCARMLSDRGQGSRQTWME
                     RKMSGWEKSFLDRYSRALWWFLRFRWVSALIWVVCLAGTVWLFMLVPKAFLPPGDSSF
                     IFGLMIGREGASPGQMHELQNQADEILRADPSVRATFTMTGNGQFLASNQGLLLAFLE
                     EPGNRAPIEDVAGGLMGKLARLPGVMPFMRPRPVLEISTGATNQNQGQYAFSLSGVNP
                     KQVYDVAGKLMERLRPYPGFASLSSDYFNNTPNLDIEIKREQARLNGVSEARILQLLR
                     SAYAQNYLYLIKKPEDQYQVILEAEDAARSEAQDLSLLHIKSDDGKNLVPLKTLVTWK
                     ESLGPQAVNHLNQFTSVTLFFNLKPGVAVGEATEFINKAAAEIVPATVRASLQGEAQT
                     FQDTVRHLTLLMALAVFVMYVILAILYESYLHPLTVLSTLPTALVGGLLTLWLFGEQA
                     SLYAFVGMFMLMGIVKKNGILIVDFAIHRVMTGERADQAIHDASLDRFRPILMTTLAA
                     VFGALPIALGIGADGASRRPLGLVIVGGLVVSQFITLFITPVIYLYLEQFQEKVLDRT
                     SFFRSHRTTAPTVLGTES"
     gene            9883..10521
                     /locus_tag="VF089_08230"
     CDS             9883..10521
                     /locus_tag="VF089_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6173988.1"
                     /translation="MAIPSPRTRKGASVQAHDDDPAARIIAVARQHFFAHGFRGVTMD
                     DLAGELGMSKKTLYASFPTKTDLLRAVLLDKFRRVEVDLQRIAAGFPNDMLDSLRQLL
                     ACLQRHTEEIQPPFVRDIRREAPQMFELVQTRRRQVIQTYFGSLLDECRKAGIVRKDV
                     STRLIIEILLGATEAIMNPARMAELGLTPRTGYSTIINVVFEGVLTEKGRRR"
     gene            10556..>10773
                     /locus_tag="VF089_08235"
     CDS             10556..>10773
                     /locus_tag="VF089_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173989.1"
                     /translation="MKRRFLIGGLIAASLIAAVVAWRLMQSESDPTLLTLYGTVDLRQ
                     VQLSFNNSERIAAVLVQEGDRVRQGQLLA"
BASE COUNT         2371 a         2860 c         2977 g         2565 t
ORIGIN      
        1 ggggtcaggg actgatggaa tataccttga tcgtatttct ggttgctttg gttttctggg
       61 gcggcatgag gagcactaac ataggcgatg aactggcagt gagttggagc aacgtcgtcg
      121 attgcatctc cgctccattc tcctgtggtc cgtaattacc tctgctgatc aggagcccgc
      181 atgttcgata tgagaacttt ttatagaaag ctaccacacg agaatgtctg gcacttctgc
      241 aggaaatgcg agaactggcc tcagtttgga tacggtgagc gaaaggatac ggattgccat
      301 tcggtgacct tctgccctag gtgtatccag caccatcgta caagccaatg cgaatggctt
      361 cacttggaat tgtcccccga tcgcttgtct ctgcgaaacg cgcgagggac gaacctggtt
      421 tccttgaagc tatgaagggt actccaatga agaaagtttt gatcgccgat gactatccag
      481 acatgcgtga attgctgggc aggcaaatag aatctttggg cttccttccg attctcgcag
      541 aaaacggtaa ggacgtttta gagaaagcta tccgagagaa accgcggttg atcatgctcg
      601 atatgatcat gccgatcatg gatggttggc aagccgtccg aagccttcgg gccaaccctg
      661 aaaccaagga catcccgatc ttggcagcga cggcgttatt tcaccaatcc gatctcaaca
      721 cctgcataga gcaaggttgc aacggctaca tcaacaagcc gttcaccatg atggagctga
      781 agaagaaggt cgccgaactt attcgctaaa ccaaggtggc gtcgggacgg cttcagatag
      841 atgacctatg ctgagaagat tatttgtaca gcttatcgac gaaccctgac tttgtcgatc
      901 tcagtgagta ctgagttgtc gataagagat tccggcttgg ccgctttggc gccgggatga
      961 gatgcgaagt taagcattgt ttgtacggct gcagccggga cgatggggag ccgttcccaa
     1021 gcgggtaaaa acgcttggta agagctttcc agatctttcg ggtctgtcgt cttggtatac
     1081 ttgccaatca tctgtttagt gaattccgcg tcagtgatag cgattttaat gccttcaagg
     1141 taggcgcgca aaaatcgctg tgtcggatca cgatgagtct tgagatagtc tctggtggtt
     1201 gctagggcgg cgtggatcat cggaatattc ttctccgtta cattcatcag ttctttcaat
     1261 cctgcggcgc gcgctttcaa tgttgtcggc gcggtaaata tccctgcgac tgcgttcccc
     1321 tggtttattc ccgccaaaat ttcaggcatt cccctgagat aaagaagttt gacatccttt
     1381 ccgggcgcca accccgattg ctggagcaaa acacgggcgg caaaatcggt ggttgccccg
     1441 ggcacagtga cacccagcac tttgcccttc agatcagaca tcgagtttat ctccggcttt
     1501 gcatagatcg acatgacggc tcgattgaga attcccgcga taaataccac tggctcacca
     1561 ttcaatcctg cttcgacgat ctcaccgcct gggttgatga tatcgaggtt cttggccagg
     1621 agtgcctgag tcgccgtggc cggggcgatg tagcgaagat caacattcaa gccgtgtttt
     1681 gtgaataatc ctttctccgt cgcaatccca taacggcgcg aaggcgccgc taatagcggc
     1741 ataattggcg cgtaccaggg gcagctcagc cgcgccagcc gacatcgcac cggaaagaat
     1801 gtagaacacc gataggagag cgatcaatcg atgcgcggat gcgcaagctc ggaccgagat
     1861 tttcaatagc ctcatttaac ctccgcgttc gcaatgatca tgttatctaa gctctatagt
     1921 agaacttagt ctttggtaag catgctatta aggaccaatc gcggctctct acgcgcccgc
     1981 cagcgcaata ccggactact gtttgtacaa ttgtttgatg aaccccgtgg cttcgatctc
     2041 ttggaccaga ctcatgtcca cgaatgcctt gggatcgacg gccgccgcct ttggattcct
     2101 tggtgcaagg tcgtccagca tggttttgac tccatccggg gtgggtagca aattttccgg
     2161 gaaaaccgag acataagcct ggtaggctct ttcgagacct tctggatcgg tgagcttgag
     2221 gttcttactg tagatcgcct tggttccttc tttgtcggtt ttgatgtagt gaatcgcttc
     2281 catcatggcg cgggagaact tcgtcagcgc cgctctattc gccttaacgg ccgactcttt
     2341 ggcgagtaca gcattccacc agaatggaat ttttatggat tgaatgtcga acagatcttt
     2401 gaaacccagc ttctcagctt cccggacaaa gggctccggc ataatgctga actgaatgat
     2461 tccgcgagaa atcgcagcga gtctcgctgg attgtctccg gcagtaataa tcttcacgtc
     2521 cttttccgga tcaatgccct tacgccgtaa cgccagccga agccccaaat ccgaagtgct
     2581 acccaggcgg tttactccgc ccgtcttacc ccttatttgc tcgagagtcg taatggtcgg
     2641 atgggagata acgtgatcca ggaaagtcgg caccattcct agaatcatga tgatatcggc
     2701 accggcgagg cgagaaagag caagcgtggt gaccgacgtt acgattacat ctacttcccc
     2761 cgcgagcaaa gcctggagcg caatggggct tgcggaaatg ctaatgacct cgccgttaag
     2821 tccgtgtttc ttgagcaacc ttttttcttg aaccacccaa atcgggctat atgccggact
     2881 cactccagac caggcgatcc tgattctctc ctgtgcgaag gattgatttc tttggactta
     2941 tgagcggtta gcgccatgag tacgattaga gcaatccgac taaaacatcc cagattctag
     3001 actccttcgt cgcgacgttc gatgcagcga cgacggcatg acatcgctca gattttatgt
     3061 tcccatttca atagcgataa aaagtttgtc gagaaaactt ttttcaagtc gtcttgattg
     3121 agtttggcgg ctcgagttac gtgtttggat attttttcga cttccatggc taatggatta
     3181 taagggtgat cggagccgaa gaggatcttg cttgtaccga cgccgcgaaa ggcctcgagc
     3241 agcagcggca tccaaccgac cactgacgtt tccaggaaga tgttctcgtt ctgccgagct
     3301 acggccactg ctagctttac atattccgaa acgcccatgt ggccgagaat gaccgggaca
     3361 tctctatact gcgcggcgag ctctgcgatg acccctggtg atgccttgct cgacataccg
     3421 gtgtggaaaa taatcggcac atgatagcgc cgggccgcag cataaatcgg ctccattaac
     3481 tccgcttcgt tgggatcgaa gaactcgatt tccggatgga gttttattcc cttcaatttg
     3541 agattaccca ggcaatgctc cagcgaatct gccgacgctt gcggaccaaa gttaggattg
     3601 atccggcaga aactgatgat tcgctgggga tgcttggcca tttcttccgc catgatttgg
     3661 tttgcctcgg aatagtccgt gtaggaagct cccagaggaa acgccaccgc acaatcgaga
     3721 ccggcttcgt cgaggttacg gactaaatca tcgcccagaa aactcgccac tccgtgcccc
     3781 aacggagtct tgcgccggcc cagatgcgaa tgagcgtcga tcgcatagta ctcggaatcg
     3841 ttgatgcgta aagccatcta tgcttctcct catcggccac cgcgcacgtg acagggttca
     3901 tagcctcgcg cgtgctgaaa ttccgtaatg gcctacctag agctgtttgg atcttcgatt
     3961 caacagaatc actcgatgat tgcgggcaac tcggcagcaa tctatcgaag ctctaatatg
     4021 cttcagaaga cctgtcaacc cagttggcgg ttctgtagtg cgcatatcat tgaaagtgat
     4081 cccttagaca gagtcgccgg gtgctccctc gaagccatgg ctgatgagtc tctcgctccg
     4141 ccataacagc gcactttttc gccgcaaggc gacagacaaa agcttgacat cagtggtgag
     4201 taaatgctaa ctaactggta tgggaaatgt cgcacggtta tcggcggacc agagaaagca
     4261 ggcgatcgtc gatgcggtgc gggacgtctt tgcggagaag gggtttgacg gcacgaccac
     4321 ccgggagctg gctcaggcgg cgggtgtttc ggaagcgctt atctacaaac attttcccag
     4381 caaagaatct ttatatgcgg cgatgttgga tgcttgcgct aaggggccga cttttgcgca
     4441 gttcaaacgg atcttggcgc ttgaaccgtc aacttccact ttggtcttga tggtttattt
     4501 cacaatctcg cactatgtgc tgcagcgtcc gggcgatccg aacaaggcgg cgctgaactg
     4561 ccttctggta cgcagcctcc tcgaggacgg agccttggtg cggctgacgc acaagagatt
     4621 tgccagcgcc tgggtagcga agttcgaggc ttgcgtgaag gaggcggtca agaccggaga
     4681 tatcggggcg acgccgatcc gccgcgactt gcgggtttgg tttgtgcacc acattgcgtt
     4741 ctcgttgatg ctgcacctcc gtcccaaggt gccggccatc gattacaacg tttcaaggga
     4801 tgcgctggtg gagcaggcga cgtggtttgc gctgcggggc gtcggtttcg cagaagaagc
     4861 aatcaaacgt cattacaacc ccaaagcatt atcgcttttg ggtgattaaa ttagctgtgg
     4921 tgagtagaag cttacctcag aatgtgagaa gaaaatggaa ccaaagaaaa tggcatcaaa
     4981 aaagacccat ttagaagaag agcaacaaga gaaagggctt ggcgcagagg accggttgac
     5041 tccgttgttt cagcccgaca ctctgatgtc ggaggagtac ttcgcgaact accgcagaag
     5101 gatcccgttg gaaccggaga aagccttgct gctggccgtg ttggaagacg gagtacgcac
     5161 gtttcaggac aatatctttg ctgagattgg caaaaagcga gcgctgctgg acgaagcccg
     5221 ggagtggctt ttcatggatg ccttggagca cgtgttttcg ttcaactcag tctgttcttc
     5281 gttgggactt gatccgggtt atattcgtcg cggtctcaag cgttgggagg agcacgcccg
     5341 agccgcaaca aggcagaagc agcggccttc ccaaggtgcc cccgaacgtc tcgtggcctg
     5401 aaccgatgag acgattggcg ttgacgtagg acggtgggat cgctctggaa atgattcgga
     5461 ctataggtct cctgctgctc gtcttgtcgg ccggatgtga caggcaagcg cccgcctctc
     5521 tcgagcgccc gcccgctccg gtctccgtcg ccgccgcagt tactcaagat gtgccgatct
     5581 atatcgatgc tgtcggtaag atcgtcgccc gagaggtggt ttcgatccag ccgcaggtat
     5641 cgggacggat cacgaagctc catttcgctg atggtgccga tgtcaaagct ggcgacgtgt
     5701 tgttcacaat tgacccgcgt ccttatcagg cgcacctcaa tcaggccgag gccaatgtcg
     5761 cccaggcgca ggcggccttg aatctggcca agaccaactt cgcccgggtt caaagcatca
     5821 gcgatccccg cgcgatttcg cggcaagact ttgacgcaaa gaaaaatgct gtagaggtag
     5881 ccgaagcgga gctgcggcag aatcgcgctg ctgtggaaac ggctcgcttg aatctggaat
     5941 actgcacgat acgctcgccc atcgacggcc gggccggaca gcggctggtc gatgttggca
     6001 atgtggtgac accgggtaat agccccctgc tggtcatcca aaggctggat cccatctatg
     6061 ccgattttac gatccccgag gccgatctta ccgaagtgca gcgaaatatg gcgcgccgga
     6121 ctttgcgtgc ggaggttcgg ctcgcggaag aaagcactaa agcgcgcgaa ggagatctga
     6181 cgtttctcga taattcggtg caagaaggaa ccggcactgt taagttgcgc gccactgtcg
     6241 gaaacaaaga ccggctatta tggccgggac gatttgccag aattcgtttg attctggaca
     6301 ctcatcgctc agcggttttg gttccggccg aggcgccgca actgtcggcc aagggatcct
     6361 ttgtttatgt catcaaccag gatgacagcg cggagctgcg gccagtgact atcggccagc
     6421 gccatggcga tctagtcgtc attgcgcaag gtgtgaacga aggtgagcgt gtcgtcatca
     6481 acggccagct tggtgttacc ccgggcggca aggttcagat tgctgctccc ggtgcaaaga
     6541 aagatccagt agccgcctct caatccggcg ccaagtcatg agcttctcag aattattcat
     6601 tcgccggccc gtcatgacga ccgtcttgac cgcgtcggtg gttctctttg gaattctcag
     6661 ctacctgcgc cttccggtca atgatttgcc ggcggtcgat tatcccgtca ttcaggttca
     6721 agcgaactat cccggtgcca gccccgatac cgtggcgaac aacatcgcta cgcctctcga
     6781 gcgccagttc atgcagatca acgggcttga gctggtgacc tcgaagagta cccagggaca
     6841 taccagcctg actctgcaat ttgcactgga taaaagcgtc gatgcagcgg ccaccgacgt
     6901 tcaaaccgca atttcacagg ccaccggcag tttgccggtg gatctgcctt cgccgccaac
     6961 atactccaag accaatccta acgatcaacc gattatgtat atcgccctga ccagcgattc
     7021 cgtgaccgtg ggtcagcttt acgactatgc cagcactcag gtcggccagc gtatcagcat
     7081 tctgccaggg gtcagccggg tcaatgtttt cggcgcgaag tccgcggtgc gcatcaaagc
     7141 cgatccttcg gccatgtggg cgcgtggcat ttccgttgaa gatctggctg aagcgattcg
     7201 caatggcacc agcaccatcg gcgccgggca attcgatggc gctggcggaa cgctactgct
     7261 ccgtccccag ggccaattgg atgatgccaa agagtacggc gacttgatcg ttagcagcaa
     7321 gaacggctcg ccggtttatc tgcgcgatgt cgcgcaggtt aagaactcgg tgcaggacga
     7381 gcgcatcaag atgcgcttct gggtgcgcgg ctatgacccg ccggcagcga ccgtgatcgt
     7441 cgcggttaat cggaaagccg gagccaatgc ggtggaagtc gctcaaagcg ttcgacagct
     7501 gctgccgctg atcagcaccg aattgccggg atcggtgcgg gtgactccga tctatgatcg
     7561 ctctcagacg atcattcact ccattctgga agtcgagttg accctggcta ttgcgttcgt
     7621 gctggtggtg ctcgtgatat ttgtgtttct cggccgcgct accgacactt tgataccggc
     7681 ggtggcattg ccgttgtcct tgctgatcac attcgtggtc atggatctac tagggttcag
     7741 cctcgataat ctatcgctca tggccctcac tctggcgatc ggctttctgg tcgacgatgc
     7801 cattgtcttt cttgaaaaca ccgttcgtcg gatggagcac ggccaaaaag ctcttgaagc
     7861 gacgatcgcc agcgcccgag agatcagctt cacaatcatc tcgatgacca tttcgcttgc
     7921 cgcggttttc atcccgctgg tattcatgcc cggattggtc ggcaggatct ttcgtgaatt
     7981 cgccgtcact attgtcgttg caatcatggc gtcgggattg gtgtcgctga ctttgacccc
     8041 gctgatgtgc gcccgcatgt taagcgaccg cggccaaggt tccagacaaa cgtggatgga
     8101 aagaaaaatg agtggttggg aaaaaagctt tctcgaccgc tatagccgcg ctctctggtg
     8161 gtttctgcgt tttcgctggg tctcagcgct catttgggtt gtgtgcttgg caggcacggt
     8221 ctggcttttc atgctcgttc ccaaggcttt tctgccgccc ggggacagca gcttcatatt
     8281 cgggttgatg atcggccgcg agggggcctc gccggggcag atgcatgagc ttcagaacca
     8341 ggccgatgaa attctccgcg ccgaccccag tgtcagagcc acttttacca tgacgggcaa
     8401 cggccagttt ctcgcctcga accagggcct gctgctggcg tttctcgaag aacccggcaa
     8461 tcgcgcgccg attgaagatg tagctggtgg gttgatgggc aaactagcga ggctgccggg
     8521 agtgatgccg tttatgcggc cacgtccggt gctcgagatc agtaccggcg cgaccaatca
     8581 aaatcagggg cagtacgcat tctcgctctc cggggtgaac cccaagcaag tttacgatgt
     8641 cgccggcaag ctgatggagc gtttgcggcc ctatccaggc tttgccagtt tatcttccga
     8701 ctatttcaac aatacgccga acctcgacat tgaaatcaag cgcgaacagg cacggctgaa
     8761 cggagtctca gaggcacgca ttcttcagtt gctgcgcagt gcctatgcac aaaactatct
     8821 ttatctgatc aagaagccgg aagatcagta tcaggtcatt ctggaagccg aggatgcggc
     8881 tcgctccgaa gcgcaagatt tatccttact gcacattaag tccgatgacg gtaagaatct
     8941 tgtgccgctg aagacgttgg ttacctggaa agaatcgctc ggcccgcagg cggtgaatca
     9001 tctcaatcaa ttcaccagtg tgaccctgtt ctttaatctc aaacccggag ttgctgtcgg
     9061 tgaggctacg gaattcatca acaaggcggc ggcagagatc gtgccagcga ctgtccgggc
     9121 cagcctgcaa ggtgaagcgc aaacctttca ggatacggtg cgacatctaa ccttgttgat
     9181 ggcgctggct gttttcgtca tgtacgtgat cctggccatt ctgtatgaga gttacttgca
     9241 tcccctaacc gttttatcga cattgcccac ggcactggtg ggaggtctgt taaccctgtg
     9301 gctttttggc gaacaggcat cgctctatgc gttcgtcggc atgttcatgt tgatgggcat
     9361 cgtcaaaaag aacggcatct tgatcgttga tttcgccatt catcgagtta tgaccggcga
     9421 acgggccgac caggcgattc acgatgccag cctggaccgt ttccgtccta tcctgatgac
     9481 gaccctggct gcggtattcg gcgcgctgcc gatcgcattg ggtatcggcg ccgacggcgc
     9541 gtcgcgccgg ccgctgggcc tggtaattgt tggcggactg gtcgtttctc aattcattac
     9601 cttgttcatt acaccagtga tttatctcta cctcgagcaa tttcaagaaa aggtgctcga
     9661 ccgcacttcg ttctttcgct cgcatcggac cacggcgccc acggtgcttg gcacggagtc
     9721 atgatcgatt agcagaggaa acaaaagata cagatccccg cacctgaccc caaaatcaag
     9781 aatcgttaac tgtcgtgttt tcactggctc tgctccagag cttcccctga gttgtggtac
     9841 aaaaggaaac tatatatact gagatagttt cctgggtact ttatggccat accttcgccc
     9901 agaacccgaa agggcgcctc cgttcaagcg cacgacgatg atcccgccgc ccgaatcatt
     9961 gccgtcgccc ggcaacattt ttttgcccac ggttttcgcg gcgtcaccat ggacgatcta
    10021 gccggcgaat tgggaatgag caagaaaacc ctgtatgcct cttttccgac caagaccgat
    10081 ctgctacgag ccgtgcttct ggacaaattt cgcagggtcg aagtcgatct ccaacgcata
    10141 gccgccggtt ttcccaacga catgctggat tcactgcgtc aactgctggc atgcctgcag
    10201 cgtcataccg aagaaataca accgccgttt gtccgcgata tcaggcgtga agctccacaa
    10261 atgttcgagt tggtgcaaac ccgacgacgt caggtaattc aaacttattt tggttcttta
    10321 ctggacgaat gccgtaaagc tggaatcgtt cgcaaggacg tttcgactcg ccttattatc
    10381 gaaattcttc tcggcgcaac cgaggcgata atgaatccgg cgagaatggc cgagctcggt
    10441 ctaaccccga ggacgggata ttcgactatc atcaatgtgg ttttcgaagg tgtattgaca
    10501 gaaaaggggc gcagacgatg acaggcgatg cggacagcgg gagcagcggt tgtacatgaa
    10561 acggcgtttt ttgatcggcg gactgatagc ggcgagcctt attgccgcag tcgtagcatg
    10621 gcggctgatg caaagcgaga gtgatcccac gcttctgacg ctttacggca ctgtcgatct
    10681 ccggcaagta cagctttctt tcaacaacag cgagcgtatc gcagctgttt tggttcagga
    10741 gggcgatcgt gtcaggcagg gtcagttgct ggc
//
LOCUS       DASUTG010000359         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1687982, whole genome shotgun sequence.
ACCESSION   DASUTG010000359 DASUTG010000000
VERSION     DASUTG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1687982"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF089_08240"
     CDS             <1..250
                     /locus_tag="VF089_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6173990.1"
                     /translation="TNTSVNPARSIGPAVFVGGWALSQLWLFIVAPIIGAPIAAGLYT
                     ASHKPVEVISAREAERALATEQVQDGRLRSVLEGRRAA"
     gene            419..601
                     /locus_tag="VF089_08245"
     CDS             419..601
                     /locus_tag="VF089_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6173991.1"
                     /translation="MKVMQDHEIGSLWKGDGLVGNERRVIKELIRKLVEERTFQVGFE
                     AALQAFGISKEEYDKL"
     gene            883..1239
                     /locus_tag="VF089_08250"
     CDS             883..1239
                     /locus_tag="VF089_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6173992.1"
                     /translation="MLATLVAAVIVAGGALAQELDGTLKKINTSGTLTIGYLTSAPPF
                     SFPGPDKRPVGYSVELCMRVASEIQKQLALENLKLNCVPVTTENRIDMMAQGKVEIDC
                     GTTTASLSRQDQVDFS"
     gene            1239..1646
                     /locus_tag="VF089_08255"
     CDS             1239..1646
                     /locus_tag="VF089_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6173993.1"
                     /translation="MIFVDAGSLLTTSDVNLRSTADLENKRIAVILGTTTERALIKLL
                     KDSFITTKLVPVKDHLEGLAAIDKGVAEAFASDRGILIGLAVTSKDPARFSLANVLFS
                     YEPYGAPQRWRIPVGGEPCACGDLSLRRHQFYL"
     gene            complement(1857..>2211)
                     /locus_tag="VF089_08260"
     CDS             complement(1857..>2211)
                     /locus_tag="VF089_08260"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6173994.1"
                     /translation="RQVTLIETESIEALRREYGVDIDAAQARRNIVTRGLALNQLIGR
                     DFAVGEVVLRGTRLCDPCAHLEKLTVKGVMLGLVHRGGLRADVLRGGIIRVGDVIGAA
                     SESAPPKIVSTVEDR"
BASE COUNT          513 a          583 c          604 g          511 t
ORIGIN      
        1 gaccaacact tccgtcaatc ccgcgcgaag catcgggcct gccgttttcg tcggtggatg
       61 ggcgttgagc caactctggc tttttattgt cgcgccgatt atcggtgcac cgatcgctgc
      121 agggctctat acagcatctc acaagccggt tgaggtgatt agcgcgagag aagcggaacg
      181 tgctttggcg acggagcagg tgcaagacgg ccgacttagg agcgttcttg aaggtcgtcg
      241 agccgcctag ccctgaccga cttccggttc ttcgcacatg aattagattt tcatcgtatt
      301 cctctttcta gccttgcgtt ctcgcgcatc gatccgcgat ttataacctg cgcccttgac
      361 aacactccaa atgttcagag catcggactg cgaaaactag tgagcttacg aggacacgat
      421 gaaagttatg caagatcatg aaataggctc actgtggaaa ggggacgggt tagtgggtaa
      481 tgagcggcgg gttatcaaag agctgatccg caagcttgtc gaggagagaa cttttcaggt
      541 cgggtttgag gctgccttac aagcgttcgg catttcgaag gaggagtacg ataagctcta
      601 aattctcgga tgccagcacc aggtcgtgcc cacaattttc atcttgcttg ccgggattac
      661 tgtatgacag gatgcgggaa ggatcgacag tttgaagaat tttcaagcaa gccggaactg
      721 taagtaagct cgaagttttt ctgcagttca ctggatgact agaaacgtaa gacaacttta
      781 cggataacaa gcgatgccaa ttgaagcgag cgagtctgct ttctagattg aatcagatct
      841 gagttcgcag ctaggagcga gaaatgaggg ggaatcaaaa tagtgcttgc cacgctagta
      901 gcagcggtga ttgttgccgg cggcgctttg gctcaggagc tcgacggaac gcttaaaaag
      961 atcaacacat cgggaactct aacgattggc taccttacgt ctgctccgcc cttttctttt
     1021 cctggcccgg acaagcgtcc cgtaggctac tcggttgaac tctgtatgcg tgtcgcaagc
     1081 gaaattcaga aacagctcgc tctggagaat ctaaagctca actgcgtgcc agtgacgaca
     1141 gaaaacagaa tcgacatgat ggcgcaaggc aaagtggaaa tcgattgcgg cacaaccacg
     1201 gccagccttt cgcgtcagga tcaggtagac ttcagttgat gattttcgtc gatgctggaa
     1261 gcctacttac cacttccgac gtcaatctcc gcagcacggc cgatctcgag aacaagcgta
     1321 tcgcagtcat cttaggcacc acgaccgagc gggcgctgat caagctcctc aaggacagtt
     1381 tcatcacgac taagttggtt cctgtgaaag accacctgga agggctcgca gcaattgaca
     1441 aaggcgtcgc cgaagcgttc gcttccgacc gcggtatctt gatcggtctg gcggtcacgt
     1501 caaaggatcc ggcccgcttc tccctagcga acgtgctctt ctcctatgag ccttatggtg
     1561 cgccgcaacg atggcgcatt ccggttggcg gtgaaccgtg cgcttgcggg gatttatcgc
     1621 tccggcgaca tcaattctat ctatgagaaa tggttcggcg ccttcggtaa accgactccg
     1681 gcgatccagg cgatgtacat gctgaacgga ctgcctgagt aaagctcgat ttcgggggcc
     1741 tgcggaaacc attatgacgt tcagaaagag ggccatgctg ggctcacagg cgcgccttat
     1801 taagtagtaa cccgagctga ttgcgctcgg cgctccatat gcgggaatct gtctatctac
     1861 cggtcttcaa ccgttgagac gattttagga ggcgctgact cgcttgcagc gccaatcaca
     1921 tctccgaccc ggataattcc gccgcgcaag acatcggcgc gcaggccgcc gcgatgaact
     1981 agccccagca taactccttt gaccgtgagt ttttccaaat gcgcgcaagg atcacacagc
     2041 ctggtgccgc gtaacaccac ctctccgacg gcaaaatctc gaccgatcaa ctgattgagt
     2101 gcgagtcctc gagtcacgat gttgcgtctg gcttgcgctg catcgatgtc gaccccatat
     2161 tctcgtctca gagcctcgat ggattcggtt tcgatcaacg tcacttggcg g
//
LOCUS       DASUTG010000360         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1242661, whole genome shotgun sequence.
ACCESSION   DASUTG010000360 DASUTG010000000
VERSION     DASUTG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242661"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF089_08265"
     CDS             complement(<1..399)
                     /locus_tag="VF089_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6173995.1"
                     /translation="MIDTSKTVGVVGLGVMGFDIAFLYAQKGYRTLVYDASEAAMQNL
                     VSRRDQTMERLTRRNRISEIEIEKVKKHLVPAARLSDMTNASLVTEAISESGNIKRSV
                     YRTLREIGFSGVLTTNTSSLTRKSLIESGDY"
     gene            complement(409..1278)
                     /locus_tag="VF089_08270"
     CDS             complement(409..1278)
                     /locus_tag="VF089_08270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6173996.1"
                     /translation="MDQIKIGIGVSSIGRFLPLIALAAGLFERKNIAVEVVNRQDEEK
                     IVDDIVAGVTPVGTPNAPSLIFSLLAGNDLVIVGGVINRPAFFLAANAAIKGIADLKG
                     KRIGINQPRRMAGMVMLALLRRWGFDIQRDLTLIDLGLNDRSLEALRDNQLDAALLPP
                     EKAFLAESEGSSIIADSSELDFHWVPLATTRRFLSDNRELVAKIAAVYVESIRVFKTQ
                     REDTLKEIGRWLPALAKKPEVLAKCYRLFADRFEESLAPSLTSISSIVKEAALQNPSA
                     HRIRAESMVEKVL"
     gene            complement(1283..1669)
                     /locus_tag="VF089_08275"
     CDS             complement(1283..1669)
                     /locus_tag="VF089_08275"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6173997.1"
                     /translation="MVEMKNPIKEADAHPDIRHLSLWRTKLAYVWVINCEPGIQDDMH
                     YHENDDHIFMVLEGECTVRTPHKEFVLKQHDTVMLESKQPYQLCNTGKGRLLLLGAGN
                     AGVNGVPRSRVPDVASHTPVQQPVVA"
     gene            complement(1734..2006)
                     /locus_tag="VF089_08280"
     CDS             complement(1734..2006)
                     /locus_tag="VF089_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6173998.1"
                     /translation="MALFYEDLIEPTIVKQVSKEVAYCRCTRSKNLPFCDGSHAGTNI
                     TPRVLEFEEPRTIAICRCWRSKDHPYCDGTHGRLVKPKERPKRGHE"
     gene            2371..2443
                     /locus_tag="VF089_08285"
     tRNA            2371..2443
                     /locus_tag="VF089_08285"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2404..2406,aa:Phe,seq:gaa)
BASE COUNT          536 a          694 c          627 g          658 t
ORIGIN      
        1 atagtcgccg ctctcgatca ggcttttccg ggtcagtgac gatgtattgg tggtgagaac
       61 tccactgaag ccgatttcgc gcagtgtccg atacacggat ctcttgatat tgccggattc
      121 cgatatggct tcagtcacca gagaggcgtt cgtcatgtcg gacagcctcg ccgccgggac
      181 tagatgtttc ttgacctttt cgatttcaat ctcggaaatc ctgttgcgtc tcgtcaaccg
      241 ctccattgtt tgatcgcgac gggacactaa gttttgcatc gccgcttccg aagcgtcgta
      301 aaccagagtt cgatagcctt tctgtgcgta aagaaacgcg atgtcgaaac ccattactcc
      361 caacccgaca acaccaacgg ttttgctggt gtctatcata ggattttgtt ataaaacctt
      421 ttcgaccatg gattcagctc tgatgcgatg cgcgctcggg ttctgtaagg ccgcttcttt
      481 aacgatggag ctgatagagg tcaaggaagg cgctaacgac tcttcgaacc ggtcggcgaa
      541 aagacgataa cacttggcta acacttccgg ctttttcgct agcgccggca gccaacgacc
      601 gatctccttc aatgtatcct ctctctgagt tttgaagacc cgaatgctct cgacgtaaac
      661 ggcggcaatt ttagcaacca gttctctgtt atcggacaga aatcgccggg tggtggcgag
      721 cggcacccaa tgaaagtcaa gctcagagct atcggcgatg atgctagaac cttccgactc
      781 cgctaagaac gccttctcgg gcggcagtag cgcagcatcc agctggttat ctctgagtgc
      841 ttcgaggctt cgatcattga ggccgaggtc gatcagcgtc agatcgcgtt gaatatcaaa
      901 gccccagcgc ctgagcaatg cgagcattac catacccgcc atgcgacgtg gttggttgat
      961 cccaattcgc ttgcccttaa gatcggcgat gcctttgatc gcggcattgg cggctaaaaa
     1021 gaatgccggc cggttgatta ctccgccgac aatcaccaga tcgttccccg cgagtaatga
     1081 aaagattaaa gacggagcat tgggtgttcc cacaggcgtc acgcctgcga caatatcgtc
     1141 gacgatcttc tcttcatcct gccggttgac cacctcgacg gcgatgttct tcctctcaaa
     1201 taatcctgcc gccagcgcga tcaaaggtag aaagcggccg atgctggaaa caccgatgcc
     1261 aatttttatt tggtccattc gcttaagcga cgacaggttg ctgcaccggc gtatgactcg
     1321 ctacgtccgg tactcggctg cgtggcacgc cgttcactcc tgcatttcca gctccaagta
     1381 gtagcagtcg tccctttccg gtgttgcaca gttgataggg ctgcttggat tccagcatca
     1441 ccgtgtcgtg ctgcttgagc acgaattcct tgtgtggtgt gcgtactgtg cactcaccct
     1501 cgagaaccat gaagatatga tcatcgttct cgtgataatg catgtcgtct tggattcccg
     1561 gctcgcagtt gattacccat acataagcaa gcttagttct ccacaaactc aaatgccgga
     1621 tatccggatg ggcgtcggcc tccttaatcg gattcttcat ctccaccata aatcctcctt
     1681 cttcgttcaa cttgtttcgc tcgttcggct tattcgatcg tataaagccg agctcactca
     1741 tggccccgtt ttggacgctc tttgggtttt accaagcggc cgtgcgtgcc gtcacaatag
     1801 ggatggtctt ttgacctcca acaccggcaa atagcaatgg ttcttggctc ctcgaattca
     1861 agcacgcggg gggtaatgtt ggtgccggca tgagagccat cgcagaaagg cagattcttg
     1921 ctgcgggtgc agcggcagta cgcaacctct ttgctcacct gcttgacgat cgtcggttcg
     1981 atcaaatctt cgtagaataa tgccactcct gtttcctttc gtttttggat cgcctgacct
     2041 gtctctggcg tgtctggtcg ctttagtaag ggatttctta aaatgcgcgc aaaggcaagc
     2101 tataagcccc gttgaccttc gtcaaggctt gatgggtgca tggaacttag gacctaagtc
     2161 agaactgcaa tgatctcgct cgttccacct tctgcttacc gagactggag ctggctcacg
     2221 cgcggataag acgactcacc aggaatatct ctcgctcacc ccagggtcgt tgcatctcca
     2281 acgctcgatc ggaggcgcag gacgtcggca tatcttccca tctcttgaat acccacagca
     2341 aacccgctag gtaattgttc ttacgcgtat ggccaggtag ctcagtcggt agagcagagg
     2401 actgaaaatc ctcgtgtcgg cggttcgatt ccgtctccgg gcacatcttt tatcagcttg
     2461 cccaatttac tttcagggtg ttagccctga agcttttttc tgtcttgcag accca
//
LOCUS       DASUTG010000361         7170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1094868, whole genome shotgun sequence.
ACCESSION   DASUTG010000361 DASUTG010000000
VERSION     DASUTG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094868"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1089
                     /gene="msrP"
                     /locus_tag="VF089_08290"
     CDS             121..1089
                     /gene="msrP"
                     /locus_tag="VF089_08290"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX6173999.1"
                     /translation="MAAIIVQPKWQTPEPSITPESTFCARRSFIKTLGLCGLGYIAGI
                     PLGCGPNENAPMIGAQENPPVADLYPGRWNPNFILDRPITNEAHAASYNNFYEFSVFK
                     GSVYKRAARLRTSPWQVEVAGLVEKPRIFDVDDLLRAMPLEERRYRFRCVEAWAMAVP
                     WTGVPIRAFLKLVKPLSAARYVRFASFLAAAEAPNQSPSYGPWPYTEALTLAEANNEL
                     SILACGIYGHPLPKQHGAPLRLVVPWKYGFKSIKSIVKIEFTAEQPRTFWNSNRPHEY
                     GFEANVDPTTPHPRWSQESEKLIDTGERRPTLPYNGYGEWVAALYT"
     gene            complement(1214..2323)
                     /locus_tag="VF089_08295"
     CDS             complement(1214..2323)
                     /locus_tag="VF089_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX6174000.1"
                     /translation="MSLPPKLDPPTRTLLGPGPSPVSSRVLQALSLPVVGHLDPEFLK
                     IMDQSMAMLREVFQTKNRLALPMSGTGSAGMETCFVNIIEPGDSVLIGVNGVFGTRMV
                     DVAERCGAQVDTVQAEWGSALDPQRFRAALAKKKYKLTALVHAETSTGVLQPLDEIAR
                     LVHENGSLLLVDTVTSLGGAPVRIDELGIDICYSGTQKCLGCPPGLSPVTFNERAIEV
                     IHNRKRKVQSWYLDLSMIEKYWGSERIYHHTAPISMNYALHESLRIVLEEGLEASWQR
                     HRQVHEIFIREMQKLELEPAVAESIRAPMINTVKIPERIDDAKVRQRLYEEFNIEIGA
                     GLGPLKGKVWRVGLMGHGSRVENVELLAKALKQLI"
     gene            complement(2349..3236)
                     /locus_tag="VF089_08300"
     CDS             complement(2349..3236)
                     /locus_tag="VF089_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6174001.1"
                     /translation="MKICHYENNQAGVIVEDKVYNFGDALIKAGLARHGYTMLEIIDA
                     LANDSKAMQIARDASQDGAGIPLKAVKLLAPITNPGSLWAAAANYRAHQKEMIERMGS
                     ADRITKTKDELMAEFFLKTTSSIIGPGDTIILPKISKLVDFECELCAVIGKRARKVTE
                     EQALDYVFGYTICWDISQRDPWGKGVQNTRNIRKGFDTFSALGPWIVTRDEIDEPQDL
                     TIKVLQNGKEAMTAHTSDMICGLRDHIRFLTSCLTLRPGDLITTGTPAGVSKLNHGDH
                     LHGAIEKIGSMELRVKAEE"
     gene            complement(3467..4507)
                     /locus_tag="VF089_08305"
     CDS             complement(3467..4507)
                     /locus_tag="VF089_08305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174002.1"
                     /translation="MDDYSMKFGAHYLPTYVPDLDGPLPEYYRTMFAQMDEMDQLGYD
                     HIWVTEHHFAYYGGTLPHPPTFMAAVARTTKQIRLGVAINVLPLHNPIDVAESYAMVD
                     VISNGRLDFGVGKGSESHEYRKFGIDQQEATGRMYEGTEVILQAWSDKPVSFNGEFFR
                     YDNVPVFPKPVQRPHPRFWVGCARSEDSFRWAGEKNFHLMTLPYIYREPDVLPKFVKS
                     YRKAQAKAGHDFTHTDVLGKFHIYVSGSLDQAIREAEPYLNNYFDVHAVADPDRKEQG
                     LLTQRDARTQLAEGFVLAGDPQRVVDTIRKWRDDVGLTTISGTFHFGGMPQELALKNI
                     RLFAEKVLPELK"
     gene            5040..5444
                     /locus_tag="VF089_08310"
     CDS             5040..5444
                     /locus_tag="VF089_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174003.1"
                     /translation="MILQHWKSLVAADPVDTGLAQSQFTRAASAEEKLMFAVLENAIE
                     QFQEYALAEDPKGKALLQEVEDWLWDKDSDWFFSFESICETLKFSPDYLRQGLVLWKK
                     AKIQGREITSRQHPIRRRRTKTVAAVSRLQGR"
     gene            complement(5527..6621)
                     /locus_tag="VF089_08315"
     CDS             complement(5527..6621)
                     /locus_tag="VF089_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX6174004.1"
                     /translation="MSNKKTVIGEFANFRERLPGILKAIFVGLPAGYVFDLLDTPIPW
                     MIGPMVAVATLNLIGVRMHSPPYARHLGQVILGSGVSLYFTPTVVAALAGNFPAIVAA
                     TVTVFLIGALGALMLSQVSGVERKSTFFASIPGGAMAMAVLAERYGAQIAPVAVAHSL
                     RVSLVVILIPFALTYGGIPLEASAYRPVLPLNFAILLPWLSFSFILGEVSERMRFHNG
                     CLLMPIFFGAALTVSGIELSAVPHRLMDFAQLMFGLVLGARYERAFFARYRMFIPFAL
                     LNSFFILIASVLAAGVLAWAFSLPIATMIIAMSPGGLAEMTITAQALQISVPLVVAFH
                     LFRVVMVNMGTQYLYSCAAWMFNHKRWKLG"
BASE COUNT         1753 a         1929 c         1830 g         1658 t
ORIGIN      
        1 aaatctcacc taatcccaac tcgccaagct cgcccacacc ttggttcacg agatggatct
       61 cggggccaaa ccgttgtagg ttgagcccgt cgaccgtaag ataggagcag tcaccctttt
      121 atggcggcga taattgttca acctaaatgg caaacgcctg agccgagtat cacgccggag
      181 tccacctttt gcgctcgtcg cagctttatc aaaacactcg gtttatgcgg tttagggtac
      241 atcgctggca ttccactcgg ctgcggtcct aacgagaacg cgccgatgat cggagcgcaa
      301 gaaaatccgc ccgttgcgga tttgtatccg ggtcgatgga atcctaattt cattctcgac
      361 agacccatta ctaacgaggc ccatgcggca tcgtacaata atttctatga gttcagcgtg
      421 ttcaaaggaa gcgtttacaa gagggccgcg cgactaagaa cttcgccatg gcaagttgaa
      481 gtcgcgggat tggtcgaaaa gccgcggatc ttcgatgttg atgacttact acgggctatg
      541 cctctggaag aaagacgcta ccggttccgc tgtgtggaag cttgggcaat ggccgtgcca
      601 tggaccgggg ttcctattcg cgctttcctg aaactggtta agcctttgtc agcagcgcgg
      661 tatgtccgtt tcgctagctt cttggcggcc gccgaggcgc caaatcaaag tccatcttac
      721 ggtccttggc cgtataccga agcattgacg ctggcggagg ctaacaatga actgagcatc
      781 ctcgcctgcg ggatctacgg acatccattg ccaaagcagc acggcgcacc actgcgactg
      841 gtggtgcctt ggaagtatgg tttcaagagc atcaagtcaa ttgtcaaaat tgagttcacc
      901 gccgagcagc cgcggacctt ctggaacagc aatcgaccgc acgagtatgg cttcgaagcc
      961 aacgtcgatc caacgacacc tcacccgcgc tggtcacaag agtctgagaa attaatcgat
     1021 actggggaac ggcgaccgac cctgccatat aatggctacg gcgaatgggt agcagctctt
     1081 tacacttaac caagtacagg gctgcgcttg tttgatgctc ctaggttgtg gttcgtagcc
     1141 aaatgatcgt gatgaataga aggtattgcg catttcgaac aacccagctc ctaatggatt
     1201 tctgcgcgca ggactagatt agctgtttca gcgccttcgc caataattcg acattttcca
     1261 cccgcgagcc atggcccatc aaacccacgc gccagacttt gcctttgagc ggaccaaggc
     1321 ccgcaccgat ctcaatattg aactcttcgt acaaccgctg acgcacttta gcatcatcga
     1381 tgcgctctgg aatctttacc gtattgatca tcggcgcccg aatcgactcg gccactgcgg
     1441 gttcaagttc cagcttttgc atttcacgaa taaaaatttc atgcacttgc ctgtggcgct
     1501 gccaacttgc ttcgaggccc tcttcaagga cgatacggag cgattcatgc aatgcataat
     1561 tcatcgagat cggcgcggta tgatggtaga tgcgctcgct gccccagtat ttctcaatca
     1621 tcgatagatc gagataccag ctctgaacct tacgcttgcg attatggatt acttcgatgg
     1681 ctcgctcatt gaaggtgacc ggtgacagcc cgggcgggca acccaaacat ttttgtgtgc
     1741 cgctgtaaca gatatctata cctaactcat cgatgcgcac cggagcgccg ccgagagaag
     1801 ttacggtgtc aacgagcagc aagctgccgt tctcatggac gagccttgcg atttcatcga
     1861 gcggctgcaa aactccagtc gaggtttcgg cgtgaaccag cgccgtcagc ttgtacttct
     1921 tcttagcgag ggcggcgcga aatcgttggg gatccagggc tgaaccccac tcggcttgca
     1981 ccgtatccac ctgcgcgccg caacgttcag caacatcgac catacgcgtg ccgaaaacac
     2041 cgttcacgcc gattaaaact gaatcgcccg gctcgatgat attgacgaag caagtttcca
     2101 tacccgcgct acctgtaccg gacatcggca acgcgagacg atttttggtc tgaaaaacct
     2161 cccggagcat cgccatgctt tgatccatga tctttaagaa ctccgggtcg aggtgaccca
     2221 cgaccggcag cgagagcgcc tgcagcaccc ttgaagaaac cggactgggg ccgggtccaa
     2281 gcaatgttcg agtaggggga tcaagtttgg gtggtaagga catagggatt taccttccaa
     2341 tctttcgatt actcttcggc ctttacgcgg agttccatag agccgatttt ctcgatcgcc
     2401 ccgtgaagat ggtcgccatg gttgagctta ctgacgccgg ccggtgtgcc ggtggtgatc
     2461 aaatcgcccg ggcgaagcgt gagacagctg gtgagaaaac ggatatgatc gcgcaatccg
     2521 caaatcatat cgctggtgtg cgccgtcatc gcttctttcc cgttttgcaa aacttttatt
     2581 gtcagatctt gcggttcatc gatctcatct cgggtcacga tccacggccc caaagcgctg
     2641 aacgtatcga atcccttgcg aatattgcgg gtgttctgta cgcccttgcc ccaaggatcg
     2701 cgctggctga tgtcccagca tattgtgtaa ccaaagacat aatcgagcgc ttgttcttcg
     2761 gtcactttac gggcacgctt accgatgacg gcgcaaagct cgcactcgaa atcaacgagc
     2821 ttggaaattt tcggtaaaat tatggtgtca cccggaccga taatggacga tgtcgtcttg
     2881 aggaagaact cggccatcaa ttcgtctttg gttttggtga ttcggtctgc gctgcccatg
     2941 cgctcgatca tctctttttg atgcgctctg taatttgccg ccgccgccca caatgatccc
     3001 ggattcgtaa taggcgccaa aagcttcact gctttgagcg ggatccccgc gccatcttgc
     3061 gaagcgtctc gtgcaatctg catcgctttg gaatcgttgg ctagtgcatc gattatctcg
     3121 agcatcgtgt aaccgtgacg ggccagccca gctttaatca aagcatcgcc aaaattgtaa
     3181 actttgtctt caacaatgac gcctgcctga ttgttttcgt agtggcagat tttcatacct
     3241 gattctccaa tccgtgacac gcggtaggtc tgttgcgagc ttcaacgtct catacctgat
     3301 ggttttagca aagtccaatg atgcggcgtt caataaccga gtctgccttt gaactggcgc
     3361 aggcgttctc agatggttca agttgattcc gatccgagct attcaaaaaa acgaaagccc
     3421 gagactgagc tcgagctttc gaattgcgat gatcgaggct gcttaactac ttcaactcgg
     3481 gcaacacctt ttcagcaaac agacggatgt ttttaagcgc cagttcttgc ggcatgccgc
     3541 caaaatggaa cgtgccggag atagtcgtca ggccaacatc gtcgcgccat ttgcggatcg
     3601 tgtcgactac gcgctgcgga tcccccgcca gcacaaagcc ttcggccagt tgagtcctcg
     3661 catcacgctg agttaagagg ccttgctctt tcctatctgg gtccgccact gcatgaacgt
     3721 caaaatagtt attgagatag ggctcagcct cacgaatggc ttgatccaga ctgcccgaga
     3781 cataaatgtg gaacttgccc aatacatcag tgtgggtgaa gtcatgtccg gcctttgctt
     3841 gtgccttccg ataactcttg acgaatttcg gcaacacatc gggctcgcga tagatatagg
     3901 gcagtgtcat caagtgaaag tttttctcac cggcccaacg aaacgaatct tcactgcgag
     3961 cgcatcccac ccagaatctt ggatgtggac gctgtaccgg ctttgggaaa accggcacat
     4021 tatcgtatct aaaaaattcg ccgttgaaac taacgggttt gtcggaccac gcctggagga
     4081 tgacctcagt tccttcatac atgcgcccag ttgcttcttg ttgatcgatg ccgaattttc
     4141 ggtattcatg cgattcactt cccttgccca ctccaaaatc caatcgcccg ttggagatga
     4201 catcgaccat ggcgtaggac tctgcgacgt cgatcggatt atgcagcggt aggacattaa
     4261 tcgccactcc caggcgaatc tgcttcgttg tgcgcgcaac ggccgccatg aacgtgggcg
     4321 gatgcggcag cgtgcctcca taatacgcaa aatgatgttc ggtaacccag atatgatcat
     4381 atcccagctg gtccatttca tccatctgcg cgaacattgt tcgatagtat tccggtagag
     4441 gaccatcgag atcgggaaca taggtaggta aatagtgcgc cccgaatttc attgagtagt
     4501 catccatgaa ttccacttcc tccgggtaac cgagcattaa agctaaatat ataggttggg
     4561 tgaacttcgg agtcaacgat ctgttggttc ggtcgcttac ttttacctct cggccgcgcc
     4621 cagggaacta ggttatagtt aagccacgca atgaaggtcg acaaaatcca atctcattca
     4681 tctggaatat ggcagtgagc acctccgcct gtcagagggc gccacagtcc gcgggattct
     4741 tcgataactt gcatcgggca tatgaggcag tgacgacggc tacttttgtc atgccaagtt
     4801 ctcttttttc ctcaatacga gagtaagctg cgcggcacac tggttgcgct aactccggac
     4861 gaaggcttgg ttccaggaaa tttcctgacc aggttttcac ccaaaggcat gggcaggcag
     4921 agcacccgta acatcacatc cggcgagctc tccttcgatc gagtctctcc tgcccgcctg
     4981 cggacactcg cgcaacagca atgtaaatat gcggcactgc cgcttgagga ccctagtaca
     5041 tgattcttca acattggaag tccctcgttg cggccgatcc ggtggatacc ggcctcgctc
     5101 agagccagtt taccagagct gcgagtgcag aagaaaaact tatgttcgcc gtgctggaga
     5161 atgcgattga acagtttcag gagtatgctc tggcggaaga tccgaaggga aaggcgctgc
     5221 ttcaggaggt ggaggactgg ttatgggata aggacagcga ctggttcttc tcgttcgaaa
     5281 gcatctgtga aacattgaag ttctcacctg attatcttcg gcaaggcttg gtcctttgga
     5341 agaaagctaa gatccaagga cgtgaaatca cctctcgtca gcacccaata cgacggcgga
     5401 gaaccaaaac tgttgccgca gtctcgagat tacagggaag gtaaggtccg cttcggccgc
     5461 tctggaacaa cgcacccgag taactgagca gtcaatccgc ctatccgaaa tctctcggtc
     5521 gtgccatcaa cccaatttcc aacgtttgtg attgaacatc catgcggcgc atgagtatag
     5581 atactgtgtt cccatattaa ccatcaccac gcgaaatagg tgaaaggcaa ccactaaagg
     5641 aacactgatc tgcagggctt gcgctgttat cgtcatctct gccaaacccc cgggagacat
     5701 ggcaatgatc atggttgcga tcggtaagct gaacgcccag gcgaggaccc cggcggcgag
     5761 gacagaggcg atgagaatga aaaaggagtt gagtaaagca aaaggtataa acatgcgata
     5821 gcgagcgaag aacgcgcgtt cgtaacgggc gcctagcacg aggccaaaca tgagttgggc
     5881 gaaatccatc aaccggtgcg gcaccgccga aagctcgata ccgcttaccg tgagcgcagc
     5941 accgaaaaaa atcggcatca acaagcagcc gttatgaaat cgcatgcgct ccgaaacttc
     6001 ccccaagata aagctaaatg acaaccacgg taataggatc gcgaaattaa gcggcagaac
     6061 cgggcggtac gcagacgctt ccaagggaat tcctccgtaa gtgagcgcaa acgggatcag
     6121 aataacaacg agcgaaacgc gcaggctatg cgcgaccgcg acgggcgcga tctgagcccc
     6181 gtagcgctcg gccaacaccg ccatggccat ggcgccgcct ggaattgaag cgaagaaggt
     6241 tgattttcgt tcaactccgg aaacctggct cagcatcaat gcgccgagcg cgcctatcaa
     6301 gaaaaccgtt actgtcgcgg caacgatcgc gggaaagtta cccgccagcg cagcgacgac
     6361 cgtcggcgta aagtagaggg acactcctga accgagaatt acctgaccca aatgacgcgc
     6421 atatggaggc gagtgcatac gcacaccgat caagttcaac gttgcaacag cgaccatcgg
     6481 tccaatcatc cacggaatag gtgtgtctag caggtcaaat acatatcctg ccggaagacc
     6541 gacaaagatt gccttcagaa tgccagggag tcgctcccga aagttggcaa actctccaat
     6601 tactgttttt ttattgctca cgtttgttgt tcctcgacag caagagaaca agccgacgca
     6661 aacttgaaag gcgattaaca tgccagccga gcctcgagac tgacaaggct gggaagtact
     6721 tctagcagtg ctcttgccga aaactatctc tcaatcaccg aatgaaacag tctgacgcag
     6781 gccgtcaaat gacggatcca attcgcgtct gagaaaactc gccatcgccc ttcttcgggc
     6841 gctctatcat tcttcttctg aagaatgcag aagagcaccc ccgctcgaaa taactcgctt
     6901 aacatgcgct gcagattcgc tatcgcactc accactgaaa tctcgttttg cacctcacgt
     6961 caacggggtt atcgattcat tgaacgtagc cccctgatga agcatttgcg gtttgctgtt
     7021 agctctggtt gtttcttgac tccatgtaaa tcaaagaata agggtgcatc taagtgatag
     7081 tcgtttggca atagaaagac taaggagcgg gcattcatta agatcctgcc gagttttctg
     7141 gcgactagag gttaaaggtc ctaatcagag
//
LOCUS       DASUTG010000362         3415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1837569, whole genome shotgun sequence.
ACCESSION   DASUTG010000362 DASUTG010000000
VERSION     DASUTG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837569"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VF089_08320"
     CDS             <1..1427
                     /locus_tag="VF089_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174005.1"
                     /translation="LKRQRKHRLNNLSLAEKGRGRIKRHLMGHLSILSRAEVESLGPP
                     TPEELSETGVPDAFLCDLALKHVAMLPEPTTTSVADRLHLPRALTEELLYHLYREKLI
                     EMRLQSAVGNTRYAMLDHGWEKVSRLQSQSGYLGPAPVSLDDYAHMMKLQAVPSRPAS
                     MEAVRNAFRDLILPDSLLQTLGCVINSRSSLFLTGLPGTGKTAVSERMNAALFGHIWI
                     PYAVEIDGQIIRIFDAHCHRAVPPRETALEHDRRWVEIQRPLVIVAGELTLDNTDLAW
                     SESAKFYEAPFQVKSNGGTLVIDDFGRQRVQPQELLNRWILPLERRVDFLTLHNGKKI
                     EVPFEQLVVFSTNLEERDLVDEAFLRRMGYRARVEPPTPAAYVEIFKRVANVRGLAVD
                     QESINHLLNKYVIEKRMLKGCEPRDLLNKVKDICVFEGQALHLTPELIDLAWGNYFGT
                     AHGFAPEVQQFQAAAAGAEPLPMQ"
     gene            1486..2622
                     /locus_tag="VF089_08325"
     CDS             1486..2622
                     /locus_tag="VF089_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6174006.1"
                     /translation="MIRIATHIVLLFILLLIFSSLKIGRTLRASPIFWDNPPTEYRTI
                     KARSGDTLKKIAQRLDIPVAELVRLNGLSEKTRLPKGTRIQAPIPAATPNTDAVIVGN
                     RITLSDGYSFEADEVWKNGDEIWFRKGKISQRLEKPVSSVKPVMKASEPPVDSPLHAS
                     RSSKPLIAEPTPNIWIHLVGGARFRVDEVQETSDGAWYTRANLSIFMDRNRIARIERE
                     LPQAAGGASRNRDWTSGNAWIDGLIKTNGDRYGLDPYLVFLVIEQESQFRTRAVSPKG
                     ARGLMQLMPGTARRLGVKDSFDPAQNIMGGARYLKELMTMFGGRVDLALASYNAGEGA
                     VMKYGRAVPPYRETRDYVKKITKRYGHQENAKKDGKPPDASPAP"
     gene            2644..>3415
                     /locus_tag="VF089_08330"
     CDS             2644..>3415
                     /locus_tag="VF089_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174007.1"
                     /translation="MKYPFLRTILSLLFLFCFSGDIALSQITRDAPLNNAAVVKLVKA
                     GFKEKTILSIISARPSNFDLSTERMIDLKRSGVSERIILAMIARQDGMIAIDDSWAED
                     PFFRDGVEPKANNPLKAPSSGDSSSTDIFGSSGGVKGETRSRGGNGSIVDDTITTGSA
                     TVRIIKPPAEANTPAKLERNPTLTNTSIIELVEAGFSEGTIIRRIDQSAVDFDLSAGQ
                     QKELRRRRVSDKVIAAMQAAMGLESDTAKPPSGNNAKPK"
BASE COUNT          842 a          903 c          924 g          746 t
ORIGIN      
        1 ccttaaagcg acagcgaaaa caccggctga ataacctatc actcgcagaa aagggaagag
       61 gaagaatcaa gaggcacctc atgggacatc tcagtatttt gagtcgcgcg gaagtagaaa
      121 gccttggtcc acctactcct gaagagttaa gcgagaccgg agtacccgac gcctttctat
      181 gtgacctcgc ccttaaacat gtcgccatgc tgccggagcc aaccacaacc tccgttgcgg
      241 accgcctgca tcttccgcgc gcgcttactg aagaactgct ctatcaccta taccgtgaaa
      301 agttgatcga gatgcgattg caaagcgcgg ttggcaacac tcgctatgca atgctcgatc
      361 acggttggga aaaagtaagt cgattgcagt ctcagagcgg ctatcttggc ccggcgccag
      421 tttctctcga cgactacgca cacatgatga agctgcaggc ggtgccgtcc cgtccggctt
      481 ctatggaggc agtgcggaac gcttttcgtg atttgatact cccggactcc ctactgcaaa
      541 cactgggctg tgtcatcaat tcacggtcat ccctgttttt gacgggactt cccggtacgg
      601 gaaagactgc cgtatctgag cgaatgaacg cagccttgtt tgggcatatc tggatcccct
      661 atgccgtgga gatcgacggc cagatcattc ggatctttga cgcccactgt cacagagcgg
      721 tccccccgcg agaaactgcc ctcgaacatg atcgtcgatg ggtcgaaatt caaaggccgc
      781 tggttattgt ggcgggtgaa ttgacactcg acaatacgga tctcgcatgg tcggagtcag
      841 cgaaatttta tgaagcgccc tttcaggtga agtccaacgg gggcactctg gtgattgatg
      901 actttgggcg gcaacgcgtg cagccccagg aactactcaa tcgctggatc ttgccgctgg
      961 aaagacgggt agattttctt acgcttcata atggaaagaa aatcgaggtc ccctttgagc
     1021 aactggtagt gttctcgacc aatctcgagg aacgtgacct ggttgacgag gccttccttc
     1081 gtcgcatggg ataccgggcg cgtgtggaac cgccaacacc tgcggcttat gtcgaaatct
     1141 tcaaacgggt ggcgaatgtt cgaggacttg cggtcgatca ggaaagcatc aatcacttac
     1201 tcaacaagta tgtaattgag aagcggatgc tgaagggatg cgagccgcgc gatctcctga
     1261 acaaggtcaa agacatctgt gtgtttgaag gacaagcgct gcatcttacg ccggaactaa
     1321 ttgatttggc ctggggtaac tatttcggca cggcccacgg tttcgctccg gaagtccagc
     1381 aattccaagc cgcagcggcg ggagccgaac cgctaccgat gcagtgagtc cggtgaaagc
     1441 tgttgccggc gagctctgga atgacaggcg taaaacatac gatcgatgat ccggattgcg
     1501 acacacattg ttctgctgtt catcctgctg ctgatttttt cttcattgaa gatcggccgc
     1561 acccttagag cctcgccaat attctgggac aaccccccta ccgaataccg cactatcaaa
     1621 gcccgctcgg gagatacgct caaaaagata gctcaacgac ttgatatccc cgtcgccgaa
     1681 ctggtacgtc tgaacggact aagcgaaaaa acgcgacttc ccaagggtac gcgcatccag
     1741 gcccccatcc cggcggcaac acctaatacg gacgctgtga ttgtgggcaa ccgcattact
     1801 ctgtccgatg gttactcgtt tgaagctgat gaagtctgga agaatggcga cgaaatctgg
     1861 tttcgcaaag gcaaaatttc tcagcgactg gagaaaccag ttagttccgt gaaaccggtc
     1921 atgaaagcga gtgagcctcc ggtagacagc ccgcttcacg catccagatc ctcaaagcct
     1981 ctaattgccg aacctacgcc caacatctgg attcatctgg tcggcggcgc gcgtttccga
     2041 gtggatgaag tccaggagac cagcgacggg gcttggtaca cgcgggcaaa tttgtccata
     2101 tttatggacc gcaatcgtat tgcccgcatt gagcgagagt tgccgcaggc tgccggcggc
     2161 gcgtccagaa atcgagattg gacctccggc aacgcatgga ttgatgggct aatcaaaacc
     2221 aatggcgacc gctatgggct tgatccgtat ctggtattct tggtaatcga gcaggagtcg
     2281 cagttccgga cacgcgcagt gagccctaaa ggcgcgcgtg gcttgatgca actgatgcca
     2341 ggaacggcac gtagattagg cgtcaaagat tccttcgatc cggcgcaaaa tatcatgggg
     2401 ggcgctcgat atcttaaaga gctgatgacc atgttcggcg ggcgtgtaga ccttgcgttg
     2461 gccagttata atgccggcga aggcgcagtt atgaagtacg ggcgcgcggt tcctccttat
     2521 agagaaactc gcgactatgt taagaagata actaagcgct acggtcacca ggaaaatgca
     2581 aaaaaggacg gcaagccccc cgatgcctct cccgcgccat aggacgcgca gaaagtgtca
     2641 ggcatgaagt acccatttct ccggacaatc ttatcgttgc tttttttgtt ctgctttagc
     2701 ggggacattg ctctgagtca gatcacccgt gatgcaccat taaacaatgc ggcggtcgtc
     2761 aagctcgtta aagcggggtt caaagaaaaa acaatactct cgatcatttc tgcccggcct
     2821 tccaattttg atctttcaac cgagcgcatg atcgacttga aacgcagtgg ggtaagcgag
     2881 cgaattattc tggcgatgat tgctcggcag gacggcatga ttgccatcga tgattcatgg
     2941 gccgaagatc cttttttccg ggacggcgtc gaacctaaag cgaacaatcc actgaaagcg
     3001 ccgtcgtctg gcgacagttc gagcacagac atttttggtt caagtggcgg cgtcaaaggt
     3061 gaaacgcgca gtcgtggagg aaacggttct atcgttgacg acaccattac cactggcagc
     3121 gccacagtgc ggattattaa gccgcctgcc gaagcgaaca ctccggcaaa gctggaaaga
     3181 aatcccactc tcacgaacac ttccattatc gagcttgtcg aagcagggtt ttcagaggga
     3241 acgattattc ggcgcataga ccaatccgct gtagattttg atctctccgc cggtcaacaa
     3301 aaagagctcc gtcggcggcg cgtcagcgac aaggtgattg ccgctatgca agctgccatg
     3361 ggacttgaat ccgatactgc caagccgcca tcgggaaaca atgccaaacc gaaga
//
LOCUS       DASUTG010000363         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_798580, whole genome shotgun sequence.
ACCESSION   DASUTG010000363 DASUTG010000000
VERSION     DASUTG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798580"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..1355)
                     /locus_tag="VF089_08335"
     CDS             complement(570..1355)
                     /locus_tag="VF089_08335"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011246674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX6174008.1"
                     /translation="MERLQKIIARAGLASRREAERWILEGRVSVNGTVVRKLGTQAEP
                     TRDSIRVDGKRLKLAAEPVYLALNKPAGFITTLNDPQQRPDITRFLEPLGEKQRVFPV
                     GRLDFNSSGLILLTNDGELAQRLMHPRFGVKKVYHVKLSACPTEIELGRLRKGIRLED
                     GISAPARVRVLEKLKKNAWVEIEIHEGRNREVRRMFEALGYFVEKLIRVRIGNVELGR
                     LALGEIRPLMRTEIIKLKRAVGLQVSRPEPERRERRRPAGSRR"
     gene            complement(1362..1973)
                     /gene="scpB"
                     /locus_tag="VF089_08340"
     CDS             complement(1362..1973)
                     /gene="scpB"
                     /locus_tag="VF089_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX6174009.1"
                     /translation="MEREDVKSILESLLFVADSPLTVQRLSEVIDGAGKDDIRSVLEE
                     LDNELQDTRRGIRLVEVAGGYQLRTAKANADWVKKFLGGRPARMGRATLETLAIVAYR
                     QPITKAEIEAIRGVDVDGVITTLLERNLVRAVARKDVPGRPFLYGTTPEFLELFNLKD
                     LAQLPTLKEMEEMTLPEIPGEQVAVEEIQEAEQPHEAEQEKQD"
     gene            complement(1963..2733)
                     /locus_tag="VF089_08345"
     CDS             complement(1963..2733)
                     /locus_tag="VF089_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX6174010.1"
                     /translation="MSTRVQLEIFEGPLDLLLHLIKKNEVSITDIPIASITEQYLATL
                     ELMETLSLDVAGEFLVMAATLIHIKSRMLLPAGADETEEDEGNDPRDELVRRLLDYQR
                     FKDAADQLEQREVLSRDVFVRSATPAEEAAAPTFRELSVFELLNALRRVIDRLPKDVF
                     HQVELDKITVREKMTLLLDKLRAQGRVFFEELFNEAKTRMDVVVTFLAMLELVKVRAV
                     RIFQDELAGPIIIEAAASMEQAAQLANAVEPEEEQHGT"
     gene            complement(2730..3707)
                     /gene="trpS"
                     /locus_tag="VF089_08350"
     CDS             complement(2730..3707)
                     /gene="trpS"
                     /locus_tag="VF089_08350"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX6174011.1"
                     /translation="MEIIVSGARPTGRLHLGHLHGALKNWIRLQEDYRCYFFVADWHA
                     LTTDYATPQGIRQSTTEMVIDWLSVGLDPARSVLFRQSQVKEHAELHLIYSMITPVPW
                     LERNPTYKEQIKELTGKDLSTYGFLGYPVLQAADITIYKANRVPVGVDQAPHVELTRE
                     IVRRFNQLYRSIFPEPEVLLTETQKLPGLDGRKMSKSYGNAVFLSDNAEEIDRKLSRM
                     MTDPARVKRTDPGEPEKCPAFQLHKIYCTPEEIEFVSTGCRTAGIGCLECKKVMIKHV
                     IEDLAPFREKRAQLEKTPGVVEEVLQAGHEAAQQKASETMREVRETLGL"
     gene            complement(3720..>4226)
                     /locus_tag="VF089_08355"
     CDS             complement(3720..>4226)
                     /locus_tag="VF089_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6174012.1"
                     /translation="GTILIPLLLLISNAPFLFGYAKPVPVNFQNLRNPKRDMIWVALA
                     GPVTNFLLALLCVLLLKFLVPMIGKSSPMGMSASLVDFFMPIIFMLERGILINIVLAV
                     FNLLPLPPLDGGRVLVGLLPEPFSTLLARIEPFGFLILLLLLMTRSLSWIIGPPLDFL
                     LKVFLELI"
BASE COUNT          942 a         1219 c         1116 g          949 t
ORIGIN      
        1 gctcaagagt agtcctaacg ctgaaaagag aagcactttt atttttggtt ttgtttccat
       61 aatttttctc tccaattttt gcagtacaac cgaccagtaa cctgctatcc tgggattgtc
      121 cttgccgtag gcgttcgtaa cacttgccac atcggggagg acctctggca ccgctactga
      181 gttgctgtat tgggagcgag tagattctga cgggaatctt agaaacacat cgagagggat
      241 cgagccggag agtgagcgat ggcttcatcg gcatgccttt catcgtgcga acagtcatca
      301 acgaccacga cagaaacagc acacacagca gccacgagta aaagtgattg ttctaacgcc
      361 gatttatcac agccttggat ctcgtcaact aaattttgtg gtgaagtttc aaccggatgc
      421 cttatgtgag ataacgaccc ttaataatct ccggagagag cggttcccgc gtctaattgg
      481 atgatgcgta ggttcggaac tgaagttgtc aattgcggga agaagaggaa agagccgcac
      541 agcgttgcag tgtacagtga cggcaaacct caacggcgag agccggcggg ccttcttctc
      601 tcacgcctct caggctctgg ccggctgact tgtagcccga cggcgcgctt gagcttgatg
      661 atctccgtgc gcatcaaggg gcggatctcg cccagcgcga gccggccgag ctcgacattg
      721 ccgatgcgca cgcgaattaa cttttcgaca aaatagccca gggcttcgaa catgcggcgg
      781 acttcgcggt tacggccttc gtggatttcg atttccaccc aggcattctt ctttaatttc
      841 tccaggaccc gcacccgcgc cggcgcgctg atgccatctt ccaggcgaat gcccttgcgc
      901 agccggccca gctctatttc agtcggacag gcgctgagct tgacgtgata aactttcttg
      961 acgccgaaac gcgggtgcat caggcgctgc gccagctcgc cgtcattggt caggaggatg
     1021 agtcccgaag aattgaaatc cagtcgtcca accggaaaga ctcgttgctt ttccccaagc
     1081 ggttccaaga accgggtgat atcgggacgc tgctgcggat cattgagggt agtgatgaat
     1141 ccggcgggtt tgttgagcgc cagataaacc ggttccgcgg cgagcttgag tcgtttgcca
     1201 tcgacgcgga tgctgtctct ggtgggctcg gcctgagtgc ctaatttgcg caccacggtg
     1261 ccattgacgc taacccggcc ttcgaggatc caacgctcag cctcacggcg cgaggccaga
     1321 cccgcgcgcg cgataatttt ctgcaagcgt tccatggccg cttaatcctg cttctcctgc
     1381 tcggcttcgt ggggctgctc ggcttcttgg atctcttcca ccgccacctg ctcaccggga
     1441 atttccggca acgtcatctc ttccatctct ttcaaggtcg gcagttgcgc cagatccttg
     1501 agattgaaca gctccaaaaa ctccggcgtt gtcccatata gaaacggcct gcccggcaca
     1561 tccttgcgcg ccactgcgcg gaccagattg cgctccagca gcgtggtgat caccccgtcg
     1621 acgtcgacac cgcgaatcgc ctcgatctcc gccttggtga tgggctggcg ataggcaacg
     1681 attgccaaag tctccaatgt tgcccgcccc attcgtgccg gccgtccgcc gagaaatttc
     1741 ttaacccagt ccgcattcgc cttggcggtg cgcaactggt acccgccggc gacctccacc
     1801 agacgaatgc cgcgccgggt gtcttgcaac tcgttgtcca actcttcgag aaccgagcga
     1861 atatcgtctt tgccggcacc gtcgataact tcgcttagac gttgcaccgt caagggggaa
     1921 tcggcgacaa acagcaggct ttcgagaatg gatttcacat cttcacgttc catgttgctc
     1981 ttcctcgggt tccacagcat ttgccagttg cgcggcttgt tccattgagg cggcggcttc
     2041 gataatgatc ggtcctgcca gttcgtcctg gaagattctc accgcgcgca ctttcaccag
     2101 ctccaacatc gccagaaacg tcaccaccac atccatgcgc gtcttggcct cattgaacag
     2161 ttcctcaaaa aacacccggc cctgggcacg cagcttatcc agcagcaaag tcattttctc
     2221 gcgcaccgtg attttatcga gttccacctg atggaacaca tccttcggta gacgatcgat
     2281 gactcggcgc agagcattta acagctcaaa gactgacaac tcgcggaaag tgggcgcagc
     2341 ggcttcttca gccggagtcg ccgaccggac aaacacatcg cggctcagta cctcccgctg
     2401 ctcgagctga tctgcggcat ccttaaagcg ctggtaatcc agtagccggc gtaccagttc
     2461 gtcgcgcggg tcattgccct cgtcctcctc cgtctcatcg gcgcctgccg ggagaagcat
     2521 tcgcgacttg atatgaatca aggtagcggc catcaccaga aattcaccgg caacatccag
     2581 actgagggtt tccatcagct ccagggtggc caaatattgc tcggtgatgc tggcgatggg
     2641 aatgtcggtg atgctcacct cattcttttt gatcaaatga agcagcagat ctaaaggacc
     2701 ttcgaatatc tctagttgaa ctcgggtgct cacagtccca gggtctcccg cacttccctc
     2761 atggtttcgc tcgccttctg ctgcgcggcc tcatggcccg cctgcaacac ttcttcgacc
     2821 acgccaggag ttttctccag ctgcgctcgc ttttctctga atggagctag atcttcgatg
     2881 acatgcttga tcatgacctt tttgcattcg agacatccga tgcccgcggt gcggcagccg
     2941 gtgctgacaa attcgatctc ttcaggcgtg cagtagattt tgtgcagctg aaatgccgga
     3001 catttctccg gctctcccgg atcggtgcgt ttaaccctcg ccgggtcggt catcatccgg
     3061 cttagcttac ggtcgatttc ctccgcattg tcggagagaa agaccgcgtt gccgtaactc
     3121 ttgctcattt tacggccatc caaacccggc agcttctgcg tctctgtgag caacacttcg
     3181 ggttcgggga atatcgaacg atagagttga ttgaaacgac ggacaatttc ccgagtcaac
     3241 tccacgtgcg gcgcctggtc caccccgacc ggaaccctgt tcgccttata aatggtgatg
     3301 tcggccgcct gcagcaccgg ataaccaaga aagccatagg tcgaaaggtc tttgccggtg
     3361 agttccttga tctgctcctt atacgtgggg ttgcgctcga gccagggcac gggagtaatc
     3421 atcgaataaa tcaagtgcag ttcagcgtgt tccttgacct gggattggcg aaacagcacg
     3481 gaccgcgccg gatcaagccc gacgctaagc cagtctatga ccatttcagt ggtgctctgc
     3541 cggatccctt gcggggtagc gtaatccgtg gtgagcgcat gccagtcagc gacaaaaaaa
     3601 tagcatcgat aatcttcttg cagacgaatc cagttcttaa gcgcgccgtg caagtggccg
     3661 aggtgcaagc gcccggtagg tctagcgccg ctaacgatga tttccatgat agtccaatcc
     3721 taaatcaatt cgaggaaaac ttttagtaaa aaatcaagcg gaggtccgat gatccaactc
     3781 aaagagcggg tcatcagcaa gagcagcaga ataagaaagc caaacggttc gatacgcgct
     3841 aggagcgtag aaaacggctc ggggagcaaa cccaccaaca cgcgcccgcc gtcgagcggc
     3901 ggcagcggca gcaggttaaa gaccgccaag acgatattga tcaaaattcc tctctcgagc
     3961 ataaaaatga tcggcataaa aaaatcgaca agcgacgctg acattcccat cggagagctt
     4021 ttgccgatca tgggaacgag aaatttgagc aacaagacgc acaacaacgc cagaagaaaa
     4081 ttggtcacgg ggccggccaa cgccacccag atcatgtctc gttttgggtt gcgcaggttc
     4141 tgaaaattca ccggcaccgg cttggcgtag ccgaacagaa acggcgcatt tgaaatgagc
     4201 agcagtaaag gaatcagaat agtgcc
//
LOCUS       DASUTG010000364         6042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1688398, whole genome shotgun sequence.
ACCESSION   DASUTG010000364 DASUTG010000000
VERSION     DASUTG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688398"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF089_08360"
     CDS             <1..690
                     /locus_tag="VF089_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174013.1"
                     /translation="DSSYSVTSVAFRQSIWTYLGRAQYSLGNFAQARESLEKALSQSN
                     EDHLARLYLGLTLLRQPVPPVPKNPFTLKDVSYALTQGVAPKRMLVLVAERGISFDLT
                     KEAENQLRSSGADATLLDELRKTAAESEKRKKAAENPAQGAKHIAAALTTLRSDLDTA
                     IYNSSQGKFWDPSGEIRKQMEVALMLLSSREPDTAKIISTSEWIGLRLEEEIDNARRD
                     ESQQRRGEQRR"
     gene            806..1441
                     /gene="pal"
                     /locus_tag="VF089_08365"
     CDS             806..1441
                     /gene="pal"
                     /locus_tag="VF089_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6174014.1"
                     /translation="MFYYKTAGYIVLSAAILTATACGSSGAKPDAGLAGTANPSSSSS
                     PPSGEGRADATRESNLSSGSFESEPKGEKSPATSPSSLEQLKDGKPTKTAASSPLKDV
                     LFEFDRHDLTAEARDTLRSNAEWLKRNASTRIEVEGHCDERGTSEYNLALAAKRAQAT
                     RDYLITLGITPDRISTISYGEEIPACIESSESCWRQNRRVRFVVAPPRAAS"
     gene            1453..2298
                     /gene="ybgF"
                     /locus_tag="VF089_08370"
     CDS             1453..2298
                     /gene="ybgF"
                     /locus_tag="VF089_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX6174015.1"
                     /translation="MYLKSPGLVKRFRVRVTAIASFALVGVSGCVQPERVEVLEREQR
                     RIQSDNVNQQKEIDATRASLADTRANLQQVEREISSLKELIEETRHQVGRQIDHTSRE
                     GDQRVKDLEARLGKLAEGFKAQGEQLKLREDELKEVRKGLQLPPEATEMASAENETIR
                     RDYEVAWRNLEKRDYRIAIARFKDFQKKHPKSSLAANAQYWIGESHYALREFDQAVIE
                     FDAVRTKHPEAANVPAALLKQAYAFAELGENTNARLLLQEVVEKYAQTPEATQAKQKL
                     KSLES"
     gene            complement(2423..3193)
                     /locus_tag="VF089_08375"
     CDS             complement(2423..3193)
                     /locus_tag="VF089_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174016.1"
                     /translation="MAQEDLHETLGLEQVVELREKTEKISRSLLGQLRDHLDTLRPLL
                     APARMFGKHVRSALREDVPDAETAIKKLCEKYNGICGKPFSLPPNLANDAVADLDGRL
                     ELYPWEYTHEAKDHDQTKTVTVSSPVRHVLTYKSSYSLAQIRLAVAGKGDRRQDDIRD
                     FLVAALAMQSALDKFPGLVRLLEELRYHVQVEKNPALGDLPLVVISSSIPSFRPSDQV
                     VLATTRLSGVPAFIELIDLDSIQRLQDPLKSRLEEALR"
     gene            3510..4304
                     /locus_tag="VF089_08380"
     CDS             3510..4304
                     /locus_tag="VF089_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174017.1"
                     /translation="MNVIALNIAFSSRAVFLLLGLLLTGRPSAAATPLKAGTVLESRG
                     IVLKQRLSEFPVTVHVILQIHEHPDPKRREIQDMGKLAACEESIVSTVLSIAENGVPA
                     VVVEGFYFAGTFDKPASIAVSEVPRSDRIQSKWILASQKNLFVYGFEVKFLNDLAGWV
                     VKELGKSADKAVQLGKEPGSTDGETNKIEVAKLVQEEVTRLNLWHAGIIPERSFLALQ
                     TALAVALARQETQVQLVIGKLHWADLVYAANRHPDVRLRLVPYECP"
     gene            complement(4588..5847)
                     /locus_tag="VF089_08385"
     CDS             complement(4588..5847)
                     /locus_tag="VF089_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX6174018.1"
                     /translation="MPKDRGDEKEGMQGRVKIKPELLDEALKEYRGPQDLEEIFKEFK
                     KAVLERALGAELSQHLGYGKGEDKPEHQTNHRNGASGKRILTDQGRLEIEVPRDRESS
                     FESQLIKKGERRFSGFDDKIIAMYARGMTVREIQGYLEEMYGVLVSPDLISQVTDAVM
                     EEVREWQNRPLERLYPVVFFDALRIKIRDEGAVKNKAVYLALGVLPDGTKDILGIWIE
                     QSEGAKFWLRVMTEIKNRGVSDILIAVVDGLKGFPEAITAAFPLTQVQTCVVHLIRNS
                     LQYAGWKDRKALVAELKTIYRADTAEKALAALEAFSQGPWGKKYPPIALIWKRQWEQV
                     TPFFSYPAEVRKIIYTTNAIESLHMQLRKIIKNRGHFPNDDAAVKLLYLALRNITKDW
                     KMSAREWKSAMNQFAILFADRFMPQLH"
BASE COUNT         1488 a         1571 c         1537 g         1446 t
ORIGIN      
        1 gattccagtt actcagtgac gtccgtcgct ttccgacaaa gtatctggac atatctcggg
       61 cgtgcccagt acagcttggg taactttgcc caggcccgag agagcctaga aaaagccctc
      121 tcgcaatcga acgaagacca tctggctagg ctttatctcg gtttgacttt gctgcgtcaa
      181 cccgtcccgc cagttccgaa gaacccattt acccttaaag acgtttcata tgctttgacc
      241 caaggcgttg caccgaaacg aatgcttgtt ctagtggcgg aacgaggaat ttcattcgat
      301 ttaacaaagg aagcagagaa tcagctaaga agcagtggag ctgatgcaac tttgctggat
      361 gagttacgaa agactgcggc cgagagtgaa aagaggaaaa aagctgctga aaacccagcg
      421 caaggcgcga agcatatcgc cgcggcgtta acgactttgc gcagtgatct agatactgcc
      481 atctacaaca gttcacaggg aaaattttgg gacccgagtg gcgaaatccg caagcaaatg
      541 gaggtggctc ttatgcttct ctccagccgt gaaccggata cagccaagat catttctaca
      601 agcgagtgga taggtctacg gcttgaggaa gagatcgaca acgctcgccg tgacgagagc
      661 caacaacgac gcggtgagca aaggcgttga tcactcgcgc gtgaaactac gtatttaaat
      721 tgacagtttt tagcctaaat ggtaaatccg cggctatctt ccagtagaat aacttctttg
      781 agttcgtgag aggagttaag ttaatatgtt ctactacaag accgccggct atatcgtctt
      841 gagtgcggct atcctcacag ctacggcttg tggttcatcc ggcgcgaagc ctgatgctgg
      901 gttggccggc accgctaatc cctcttcctc ttcttctcca ccttcaggtg agggccgggc
      961 tgatgcaacg cgcgaatcga acttatcctc cggttccttc gagagcgaac cgaagggcga
     1021 aaaatcgccg gcaacgtctc cctcgagctt ggaacaactg aaagacggaa aacccacgaa
     1081 gacggcggca tccagtcccc tcaaggatgt gctattcgag tttgacaggc acgacctcac
     1141 ggctgaagcg cgcgatactc ttaggagtaa tgctgaatgg ctgaagagga atgcgtcgac
     1201 taggatagag gtcgaaggcc actgtgacga acgtggaacc agtgaataca atttagccct
     1261 ggcggccaaa cgagcccaag ccacgcgaga ttaccttata acccttggaa taacacccga
     1321 tcgcatatcg acgatcagtt acggcgaaga gatcccggct tgcatagaaa gcagtgagag
     1381 ctgttggcgg caaaataggc gagttcgttt tgtcgtggct ccaccgcgcg ccgcttcttg
     1441 aagaagatca gtatgtattt gaaatctcct ggcttggtta agcgatttag agtccgcgtc
     1501 accgccattg cgagctttgc gcttgtaggt gtttctggct gcgtccaacc ggaacgagtg
     1561 gaggtgctcg agcgagaaca gcgtcgcatt cagagtgaca atgtcaatca gcaaaaagag
     1621 atcgatgcga cccgggccag ccttgcggat acccgcgcca atctgcagca ggtggaaaga
     1681 gaaatcagca gtttgaaaga attgattgaa gaaactcgcc atcaagtcgg ccggcaaatc
     1741 gaccatacca gccgggaggg cgatcagcgg gtaaaggatc tcgaagcgag acttggcaag
     1801 ctagcggaag gctttaaagc tcagggagaa cagctgaagc tgcgtgaaga cgagttaaaa
     1861 gaagtgcgta aaggcttaca gttgccgcca gaggcaactg agatggcctc cgcggaaaat
     1921 gaaacgatta ggagagacta cgaagttgcc tggcgaaatt tagaaaagcg ggattaccga
     1981 atcgccatcg cgcgtttcaa agactttcaa aaaaagcacc ctaaaagcag cctggcggcc
     2041 aacgctcaat actggatcgg cgaaagtcat tatgcgttaa gagagttcga ccaggcggtt
     2101 atcgaatttg acgcagtacg cactaaacat cccgaagcag caaatgttcc cgcagcgctg
     2161 cttaagcaag cctacgcctt tgccgagttg ggcgagaata caaatgcaag attactcctt
     2221 caagaagtcg tcgaaaagta cgcgcagaca ccggaagcaa cccaggcaaa gcagaaattg
     2281 aaatcactgg aatcatgagc gactcgcttt tgtgttgcct catcgactat cgcggctcac
     2341 ctcgcccagt ctaattgaca agcagtaacg tagcccacag ctaaagagtg tgccaatgcg
     2401 aagctcagga agcatctgaa gtttagcgta gcgcttcctc tagtcgagat ttcaacggat
     2461 cttgcagccg ttgaattgag tccagatcaa tcagttcaat aaacgccggc acaccggata
     2521 atctagtcgt agccaaaacc acttgatcgg agggacggaa ggaaggaata gacgaactaa
     2581 tcaccacgag cgggaggtcg ccgagagccg gatttttctc gacttgaaca tggtagcgca
     2641 attcttccaa aagccggacc agtcccggga acttatcaag agcggattgc atcgccaatg
     2701 cggccactaa aaaatcccga atatcatctt gccggcggtc ccctttgccg gcaactgcca
     2761 gtctgatctg cgcgagcgaa taggacgact tgtacgtcag tacgtgcctt accggtgagc
     2821 ttaccgtgac cgtcttggtc tgatcatggt ccttggcctc atgagtatat tcccaaggat
     2881 agagctccaa gcgaccgtcc aaatccgcta cggcatcatt ggccaaattc ggcggtaagg
     2941 aaaatggctt gccgcagatt ccgttatatt tctcgcacaa ctttttgata gcagtctccg
     3001 catcgggaac atcttcccgc agtgctgacc gcacgtgctt gccaaacatt cgcgcgggcg
     3061 ctagcagagg ccggagcgta tccaaatgat ctcttaactg acccaacaac gatcttgaaa
     3121 tcttctcagt tttttcgcga agctcgacaa cctgttcgag ccccaacgtt tcatgtaggt
     3181 cttcctgtgc cataacagct tggcatgtag cacagttcac cagtcaagaa aagcactatc
     3241 gctacgagtt tcaccccagc gaaatacgag aaccgaatca gatctggccg ccagattcat
     3301 cgtggaaccg gcgaggaaag atcatttgtg agctagagat caaacgtcca ccgcgatgtc
     3361 agatgccagc ccggccttgc ggagtttatg ttctgacgtt tctcggattt gtaagcacgg
     3421 tcttggcaag aggtctcttg aatgctgtac tccactctgc ggtgtctata caacacgcgt
     3481 ttaagcgggt gacgccttcg ggaggtttta tgaacgtgat cgccctcaat atagcgttca
     3541 gttccagagc cgtattccta cttttaggtt tgcttctgac cggacgtccc agcgccgccg
     3601 caacgcctct gaaagcgggg acggtgcttg agtccagagg tatagttttg aagcaacggt
     3661 tgagtgaatt tcctgtgacg gtccatgtaa ttcttcagat ccatgaacat cccgatccaa
     3721 aaaggcgcga aattcaagac atgggcaagc ttgctgcatg tgaggaaagc attgtttcca
     3781 ccgtccttag catcgccgaa aacggcgtcc ccgctgtggt cgttgaagga ttttattttg
     3841 ccggaacgtt tgataaaccc gcatctatcg cggtttcaga ggtgccccgc agcgatcgga
     3901 tccagtcgaa atggattttg gccagtcaaa aaaacctgtt cgtttatggt tttgaagtga
     3961 agtttttaaa tgatttggca ggctgggtcg taaaagagct gggcaaatct gccgacaaag
     4021 cggttcaact cggaaaagag cccgggagta cagatgggga gacaaataaa atagaagttg
     4081 ccaagctggt tcaggaagag gtaacgcgtt tgaacctctg gcatgcaggt attatcccgg
     4141 aacgaagctt tctggctctc caaacagcac tcgccgtcgc actcgcgcgg caggagacgc
     4201 aggttcagct cgttataggc aagttacatt gggccgattt ggtctatgca gccaatcgac
     4261 atccggacgt caggcttcgg cttgtgcctt acgaatgccc atagaaattg agtcaagtgt
     4321 tttttcgttt ggaccacttt gcgggattcg ggataactac aactgtctat tcatttcctg
     4381 aggttttggg ttctcggatc gtccttttcg tgctttatga gaacatggtg gagtagattg
     4441 tatctgtctc tcgctggtgg atcccgacgt gacgtcagcg gagattaatc ttcagaaatc
     4501 ttgaaacctt ttttctgaaa acaagtatta cagggtttaa tgtgcaaatt ggaacgtatc
     4561 cgaaactttg tgtgtgaggc aagttggtta atgtagttgg ggcatgaatc gatcggcgaa
     4621 aagaatcgca aactgattca tagccgattt ccactcccgt gcgctcattt tccaatcttt
     4681 ggtaatattt ctcaacgcca gatagagcag cttgacggca gcatcgtcgt tggggaaatg
     4741 gccgcgattt ttgatgattt ttctgagctg catgtgcagg ctctcgatgg cattggtcgt
     4801 gtaaataatc tttctcacct cggcggggta ggagaaaaac ggggtgacct gctcccactg
     4861 ccgtttccag atcagcgcga tgggcgggta tttcttgccc caagggccct gggaaaatgc
     4921 ctctaaggcg gccagggctt tttccgccgt gtcggcccgg tagatcgtct tgagctcggc
     4981 caccaatgct ttacgatcct tccagccagc gtattgcagg gaattacgaa ttaaatgaac
     5041 cacgcatgtc tgaacctggg tgaggggaaa cgctgccgtg atggcctcgg gaaatccttt
     5101 tagtccatcg actaccgcga tcagaatatc agacacaccc cgattcttaa tctcggtcat
     5161 tacccgcagc cagaacttcg ctccctcgct ctgttcgatc caaatcccga ggatatcctt
     5221 ggtcccgtcg gggagcaccc ccaaggccag ataaaccgct ttatttttga ccgctccttc
     5281 atcacggatt ttgattcgta gtgcgtcgaa gaacaccacc ggatacagcc gctccagcgg
     5341 ccggttctgc cattcccgaa cttcctccat caccgcatct gtgacctggg agatcagatc
     5401 gggcgagacc aacacgccgt acatctcttc gaggtaacct tgtatctccc tgaccgtcat
     5461 cccgcgcgcg tacatggcga tgatcttgtc gtcgaatccg ctaaatcgcc gctcgccctt
     5521 cttgatcagc tgtgactcaa agcttgattc ccggtcgcgt gggacttcaa tctcaagcct
     5581 cccttgatcg gtcaggatgc gcttcccgct tgccccgttt cggtggttgg tttgatgctc
     5641 cggtttgtct tctcctttgc cgtaccccaa gtgctggctt aactccgccc ccagcgcgcg
     5701 ctccagtacc gctttcttga actccttaaa aatctcctca aggtcttgcg ggcctctgta
     5761 ttctttcaac gcctcgtcta acagctccgg cttaattttt acccggcctt gcatcccttc
     5821 tttctcatcc cctctgtcct tcggcatatc tcctccttct ctagatatgc ctcaaacaca
     5881 gaattcctga taccttctgt tacggctgct ggtactcgta cgacgaaatc caaaagccgg
     5941 actctgcagg atgcccgact tatcctatgc ttcggtctac agcgttctta atatgaccag
     6001 cgtttctcct gcaccttatt tcagttggac aacccgtacc ct
//
LOCUS       DASUTG010000365         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_798721, whole genome shotgun sequence.
ACCESSION   DASUTG010000365 DASUTG010000000
VERSION     DASUTG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798721"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF089_08390"
     CDS             complement(<1..768)
                     /locus_tag="VF089_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174019.1"
                     /translation="MADSLRYYIAHNGGLKVMQPRGGSMETLGEFFQGKTLEYLTGSR
                     KQPEVVFAAVAFDGGYRTNDGGRSWKKIFDGDVRTFTIDPHEERLVYMGIGPIRLFRS
                     EDGGMTFEPMDSMLEFSEDVKEKWDVPPRLRGIEFPHVRHIFIHPEDRRLIFVLLEHG
                     GVLFSGDGGETWEDRTDGIVYPDMHYIDNYPGSKDRYYVSSARGFFRSDDRGKRWRRV
                     ENGMPWAYTELHCYSHEWNFVSGQPPRMVVCGGRGSPG"
     gene            complement(892..>2326)
                     /locus_tag="VF089_08395"
     CDS             complement(892..>2326)
                     /locus_tag="VF089_08395"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6174020.1"
                     /translation="LKSGQPVKMVMEYGEELTAGNPRHASIIKAKTGVKKDGTIVAHH
                     LDFIFDSGAYGSMKPAGYLIGASTCAGPYKIANCLIEERMVYTNKIPCGHMRAPGDPQ
                     GFFANESQMDMVARKLGIDPAEFKRRNLMHDGDETPIGGHVSHIRGVEALEKAIEIAG
                     YNRPRPKNVGRGLAFSEWSPSGGEGSVFVNIEEDGKVKVSSPVVDQGAGVLTVIVECV
                     GQELGVPAGEIELAQLDSTTVPSDGGVGGSRATRVYGNASYEAGVKARQEMIAAAAAK
                     LMLPAEELELADSHVIHQKSKRKMSFADVIKAKGLPITVKGYYKSAEKSHDASVSAQI
                     AEVHVDPETGQVTLKQMVSAHTTGKVINPLMHQGQIDGGVVFGLGYALQEEILFDNGK
                     IVTTNFGEFKIPNIMDIPPLKTSVIESVPHGPGPYNSLAIGEVANTPTAAAVANAVAD
                     ACGVRITDLPITAEKVYKVLQENHKGY"
BASE COUNT          458 a          732 c          593 g          543 t
ORIGIN      
        1 tccgggggat cccctgccgc cgcagacaac catacgcggc ggttgacccg atacaaaatt
       61 ccactcgtgc gagtaacagt gcagctcggt gtaggcccag ggcatgccgt tctcgacccg
      121 gcgccagcgc ttgccacgat cgtcgctgcg gaaaaatccc cgcgccgatg aaacatagta
      181 gcgatctttg ctgccgggat aattgtcaat gtaatgcatg tccggataaa cgatgccgtc
      241 ggtgcgatct tcccaggtct cgccgccgtc gccgctaaac aacaccccgc cgtgctcgag
      301 caggacgaag atcaatctgc gatcttcggg atgaatgaaa atatggcgca cgtgcgggaa
      361 ttcgatgccg cgcaggcgcg gcggcacgtc ccatttttct tttacgtctt cagaaaattc
      421 gagcatgctg tccatcggct cgaaggtcat accgccgtct tcgctgcgaa acagccggat
      481 cggcccgatg cccatgtaga caagtcgttc ctcatgcgga tcaattgtaa aggtacgcac
      541 gtctccgtca aaaatcttct tccaactacg gccgccgtcg ttggtgcgat aaccgccatc
      601 gaacgccacg gccgcaaaaa caacttcagg ctgcttacgg ctgccggtca gatattcaag
      661 cgttttgcct tggaagaatt caccgagcgt ctccatcgac ccaccccgag gttgcataac
      721 tttcagcccg ccattatgcg cgatgtaata acgcaatgaa tccgccataa gacacccccg
      781 gatcacatgt tcagcagttc aatagttcaa ccattcaatc cccacctctt tccgccctgg
      841 ctcgggtgcc gctcgcaatc gatgatcgca agaggtcacc ccggtctgcc atcaatatcc
      901 tttgtgattt tcctgcagta ctttgtacac cttctccgct gttataggca ggtcggtgat
      961 tctcactccg caggcgtcgg ccacggcgtt ggccacggcg gcagccgtcg gcgtattcgc
     1021 cacctcgccg attgccaagc tgttgtaggg gccgggtcca tgcggcacac tctcgatcac
     1081 cgaagttttt aacggcggga tgtccatgat gttgggaatc ttgaattctc cgaaattggt
     1141 cgtcacgatc ttaccgttgt cgaatagaat ttcttcttgc aacgcgtaac cgagaccgaa
     1201 cacaactccg ccgtcgattt gcccttgatg catcagcggg ttgatcacct tgcccgtggt
     1261 atgcgccgat accatctgct taagcgtcac ctgtcctgtt tccggatcga cgtgcacctc
     1321 ggcgatttgc gccgagaccg aagcgtcatg cgatttttcc gcactcttgt aatagccctt
     1381 gacggtgatc ggcaaccctt ttgccttgat cacgtcagca aagctcatct tgcgtttcga
     1441 tttctggtga atcacgtgcg aatcggcaag ctcgagctct tcggccggga gcattaactt
     1501 ggccgcggcg gccgctatca tttcttgccg cgccttcacc ccggcttcat aggatgcatt
     1561 accgtaaacc cttgtagccc ggctgccgcc gacaccgcca tcgctgggca ctgtcgtgct
     1621 gtcgagctgc gccagctcga tctcgccggc cggtacaccg agttcttggc cgacgcattc
     1681 gacgataacc gtcaaaacgc cggcgccctg atccaccacc ggcgaagaga cttttacttt
     1741 gccgtcttct tcgatgttga cgaagacact gccttccccg ccactcgggc tccattccga
     1801 aaacgccaga ccgcgcccga cattctttgg cctgggcctg ttatagcccg cgatttcgat
     1861 ggccttctcc agcgcttcca cacctcggat atgcgacacg tggccgccga tcggggtttc
     1921 gtcgccgtcg tgcatcaggt tcctgcgctt gaactcggcc ggatcgatgc cgagcttgcg
     1981 cgccaccatg tccatctggc tttcattggc aaaaaacccc tggggatcac ccggagcgcg
     2041 catgtgcccg cagggaatct tgttggtgta aaccatcctc tcctcgatca aacagttggc
     2101 gatcttgtaa ggaccagcgc aagtcgatgc gccgatcaaa taccccgccg gcttcatcga
     2161 tccataggcg ccgctgtcaa agataaaatc aagatgatga gcgacgatgg tgccgtcttt
     2221 tttcacaccg gtctttgctt tgatgatcga ggcgtgacgc gggtttccgg ccgtcagctc
     2281 ttcgccgtat tccatgacca tctttaccgg ctggccgctc ttgagc
//
LOCUS       DASUTG010000366         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_651378, whole genome shotgun sequence.
ACCESSION   DASUTG010000366 DASUTG010000000
VERSION     DASUTG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651378"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="VF089_08400"
     CDS             <1..2205
                     /locus_tag="VF089_08400"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX6174021.1"
                     /translation="RCADPAKVASVASFFVSRVDTMVDRELGNIGTPEAKSCLGKIAV
                     ANSKVVYQRFREIFHGEGFVALRQRGARVQRPLWASTGTKNPNYSDVLYVENLIGPET
                     VNTLPVETLNAFRDHGRVSDATVREGLDEAANALAQLKELGIDLNAVAEKLQDDGVAA
                     FATSFDSLMSTLDKKRSFLGGIELNRQDLHLGKLQSRVDRRLKDWQAQQFGSRLWKKD
                     STLWSADAVAELTDRLGWLEMPESMEKEVEALSQFAEKAKSDGMRHVVVLGMGGSSLA
                     PEVFQRSFGNQPNYPALMVLDSTHPAAIRSIEGKIDLAHTLFLVSSKSGTTTETNSFF
                     YYFWEKLKQLKPEPGEHFAAITDPGTPLEKMAGERKFRAVFTAPEDIGGRYSALTVFG
                     LVPAALIGLNVGDILKRARHMSQACGGSVPEPENPGLMLGAALAELTLAKRDKITFLC
                     SPSLTAFPAWIEQLIAESTGKDRKGIVPVAGEKLGAPEKYGLDRAFVYLRYESDENHE
                     LDRQVAGLEANSHPVLRIEILEKTDLAQEFFRWEIATAAAGAALGIHPFNQPDVQLAK
                     DLATKAMSEARSGNRRGAKLKEEVSASDGAATQQAASSWTSKAKPRDYVAIQAYISPT
                     PEHDAALQSISEALRNRVGTAVTVGYGPRFLHSTGQLHKGGPNTVLVLQIVDQPNEDV
                     PVPETDYTFGALIQAQALGDFTALKQRRRRAFRINLGNDTKAGLQRLAEALR"
     gene            2275..3186
                     /gene="gnd"
                     /locus_tag="VF089_08405"
     CDS             2275..3186
                     /gene="gnd"
                     /locus_tag="VF089_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX6174022.1"
                     /translation="MELGMIGLGRMGANMTERLLRGGHKLITYDHSPEAIQRCVDKGA
                     VGAHSLADFVKQLSLPRVIWLMVPSGEPVDLTIEQLLPSLLKNDIIIDGGNSHYKDSI
                     RRAGKLKQLGIHFVDAGTSGGIWGLQEGYCLMIGGEKATVDQLEPIFKTLAPENGFAH
                     VGASGAGHFAKMVHNGIEYGMLQAYGEGFEMLKASPFEFDLGKISHLWNQGSVVRSWL
                     LQLAENAFAKDPQLSSIRGYVGDSGEGRWTVLEAVEREIPASILTLSLFARFASRQDD
                     SFSAKVIAALRNEFGGHPVKTNEESSL"
     gene            3183..>4234
                     /gene="zwf"
                     /locus_tag="VF089_08410"
     CDS             3183..>4234
                     /gene="zwf"
                     /locus_tag="VF089_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6174023.1"
                     /translation="MNGDPNPHAAALAPGKRPHAAVVVIFGASGDLTKRKLIPALYNL
                     ALAKRLPERFAVVGYARSELSHEAFRDRMRDAVIEFSRTGLKGESAWRDFAANLYYVP
                     GEYEDPTGYRKLKEFVDQFDRGVRALPVRILYLAIPPDLYAPVVQQIAAVDLVRKESD
                     GEPRTRVVFEKPFGSDLESARELNRRIHEALDEKQVYRIDHYLGKETVQNIMVFRFAN
                     SVFEPIWNRRYVDHVQITAAETVGVENRGGYYDSAGAVRDMVQNHLLQLLCLTAMEPP
                     VGFSADAVRDEKMKLLRAVRPVAGEEVPQSAVRGQYGRGRINGKEVPAYRQEGGVAQD
                     SHTETYAAIKFRIDNWR"
BASE COUNT          922 a         1162 c         1268 g          882 t
ORIGIN      
        1 cgttgcgccg acccggcgaa agtggcttcg gtggcgtctt tcttcgtaag ccgggtcgat
       61 accatggtcg atcgcgagct cgggaatatc ggcacacccg aggccaagtc ttgcctcggc
      121 aagattgcgg tcgccaattc caaagtcgtc taccaacgtt tccgggagat ttttcatggt
      181 gaaggctttg tcgcgctgcg gcaacgcggc gcgcgggtac aacggccctt atgggcgagc
      241 acgggcacta agaatcccaa ctactcggac gtgctttatg tcgagaatct gatcggtccc
      301 gagactgtca ataccttgcc tgtagagacg ttgaacgcct ttagagacca cggccgggtg
      361 tcggatgcta ccgtccggga ggggctcgat gaagcggcta acgcgctggc gcagttaaaa
      421 gaactaggca tcgatctcaa tgcagttgcc gagaagctcc aggacgacgg cgttgccgcg
      481 ttcgcgactt cattcgattc gctgatgtct accttggaca aaaagcgcag cttcctaggc
      541 ggtatagaat tgaatcgtca ggatttgcat ctgggtaaac tgcagagccg cgtggatcgg
      601 cggctcaagg attggcaggc gcaacaattc ggcagcaggc tgtggaaaaa ggattcgacg
      661 ctttggtccg ccgacgccgt tgcggagctg acggaccgct tgggttggct cgagatgccc
      721 gagtcgatgg agaaggaagt tgaggcactg agccaattcg ccgaaaaagc caagagtgat
      781 ggcatgcgtc acgtagtcgt cctggggatg ggcggctcga gtctggcgcc cgaagtattt
      841 cagcgcagct tcggcaatca acccaattat ccggccttga tggtcctgga tagtacccat
      901 ccggcggcaa tcagatctat cgaaggaaag atcgatttag cgcacactct gttcttggtc
      961 tcgagcaagt ccggcacgac aaccgaaaca aattcttttt tctattattt ctgggagaaa
     1021 ttaaagcagc tgaagccgga accgggcgag catttcgcgg ccatcaccga tcccggcacg
     1081 ccgcttgaaa aaatggccgg cgagcgcaag ttccgcgcgg tttttaccgc gccggaagac
     1141 atcggcggcc gctactcggc gttgacagtt ttcggactcg tgccggcggc gttgatcggt
     1201 ttaaacgtcg gcgatatttt aaagcgcgcg cgccatatga gccaagcgtg cggcggcagc
     1261 gtgccggagc cggagaatcc cggcctgatg ctcggcgccg cactggcgga gttgacttta
     1321 gctaaacgcg acaaaataac ttttctctgc tcgccttctc tgaccgcatt ccccgcatgg
     1381 atcgagcaac tgattgccga gagcactggc aaagatcgca aaggtatcgt gcccgttgcc
     1441 ggagagaaac tcggcgcgcc cgagaagtac gggctcgacc gggcattcgt gtatctgaga
     1501 tatgagtctg acgaaaacca tgagctcgat cggcaagtgg cgggattgga agccaacagt
     1561 catccggttt tgagaatcga gatccttgaa aagaccgacc tcgcccagga atttttccgc
     1621 tgggagatcg ctaccgccgc cgccggagcg gcgcttggga ttcatccgtt taatcaacca
     1681 gacgttcagc tggccaagga tcttgccacc aaggcaatga gtgaagctcg ctcgggaaac
     1741 cgaagaggag ccaagctcaa agaggaagtt tccgcgtcgg atggtgcggc aacgcagcaa
     1801 gcggcttctt cgtggacgag caaggcgaag ccgcgcgatt acgtcgccat tcaggcctat
     1861 atcagcccga caccggaaca tgatgctgcg ctgcagagca tcagtgaagc gctgcgcaac
     1921 cgggtcggca cagccgttac ggtcggctat ggcccgcggt tcctgcactc gaccggacag
     1981 ctccacaaag gcgggccgaa caccgtgttg gttctgcaaa tcgtcgatca gcctaacgag
     2041 gatgtgcctg tgccggaaac ggactacaca ttcggcgcgc tgatccaggc ccaggctctg
     2101 ggcgatttta cagctttgaa acagcggcgc cgacgcgctt ttcgaatcaa cttgggcaat
     2161 gacacgaaag ccggactgca acgtttagcc gaagcgctgc ggtgacagcg tcggcccgtc
     2221 acttcgcaga tttctggctt tcacgcctca tcatctaaac cgggagagtg gtgaatggaa
     2281 ttaggcatga tcgggctggg gcgcatgggc gcaaacatga ccgagcgcct gttgcgcggc
     2341 ggccacaagc tgattactta cgaccatagc cctgaggcga tccagcgatg cgtcgacaaa
     2401 ggcgctgtcg gggcgcattc gttggccgac tttgtcaagc aactgagctt gccgcgagtt
     2461 atctggctga tggtgccatc cggcgagccg gttgatctca ccatcgagca gctgcttccc
     2521 agtctgctta agaacgacat catcatcgat ggcggcaatt cacattacaa agattccatc
     2581 cggcgcgctg gaaaattaaa acagctgggc attcacttcg ttgacgcggg gaccagcggc
     2641 ggcatctggg gtttgcaaga aggttactgt ctgatgatcg gtggcgaaaa agcgactgtc
     2701 gaccagctgg agccgatatt taagacattg gcgccggaga atggttttgc tcacgtcggc
     2761 gccagcggcg caggacattt tgctaaaatg gttcacaacg gcatcgagta cggcatgctg
     2821 caggcctatg gcgagggctt cgaaatgctc aaagcctccc cattcgagtt cgatcttgga
     2881 aagatttcgc atttatggaa tcaggggagc gtcgtgcgct cgtggttgct gcaactggcc
     2941 gagaacgcgt ttgccaaaga cccgcaactc tcttcgattc gtggttacgt cggagattcc
     3001 ggcgaggggc gttggacagt gctggaagcc gttgagcgag agattccggc ttcgattctt
     3061 acactctcgc tgttcgctcg tttcgcctcg cgccaggacg attccttttc cgccaaggtc
     3121 atcgccgctc tgcgcaacga gtttggcggc catccggtaa aaaccaatga ggagtcgtcc
     3181 ttatgaacgg cgaccctaat cctcacgcag ccgcactggc tccgggcaag cgtccgcacg
     3241 ccgcagttgt cgtcatcttc ggcgcttccg gagatctcac caaaagaaaa ctgatccccg
     3301 ccctctataa tcttgctttg gcgaaacgct taccagagcg attcgctgtc gttggctatg
     3361 cccgcagcga gctcagccat gaagcctttc gcgacaggat gcgcgatgcg gtaatcgagt
     3421 tttcccgcac cggactcaaa ggtgaatcgg cttggcgcga tttcgctgct aatctctact
     3481 acgtccctgg cgagtacgag gatccgactg gataccgaaa acttaaagag ttcgtagatc
     3541 agttcgatcg cggcgtcagg gccttgcccg ttcgaatttt gtacctggcg atcccgccgg
     3601 atctctatgc cccggtggtt cagcaaatcg cggcagtgga tttggtgcgc aaagagtctg
     3661 atggagaacc acggacccga gtggttttcg aaaaaccctt cggcagcgat ctcgaatcag
     3721 cgcgcgagct aaaccggcgc atccatgagg cgctggatga aaaacaggtt taccgcatcg
     3781 accattacct cggtaaagag acggttcaga acatcatggt ttttcgattt gccaattccg
     3841 tgttcgagcc gatctggaac cggcgctatg tcgaccacgt tcagattacc gccgcggaaa
     3901 ccgtcggcgt ggagaatcgc ggcggctact atgatagtgc cggggcggtg cgagacatgg
     3961 tgcaaaatca tctgctgcag ttgctttgcc tgacggccat ggagccgccc gtaggctttt
     4021 ccgcggatgc ggttcgcgat gaaaagatga aactactgcg cgcggtgcgc ccggtcgctg
     4081 gcgaagaggt gccgcagagc gcggttcgcg gtcagtacgg gcgtggcagg atcaacggca
     4141 aggaagttcc agcttaccgc caggagggag gcgtcgccca ggattctcac accgaaacct
     4201 atgcagcgat aaagtttcgc atcgacaatt ggcg
//
LOCUS       DASUTG010000367         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_799209, whole genome shotgun sequence.
ACCESSION   DASUTG010000367 DASUTG010000000
VERSION     DASUTG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799209"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF089_08415"
     CDS             complement(<1..213)
                     /locus_tag="VF089_08415"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX6174024.1"
                     /translation="MQQDLNRVIEQVSKEKGIDKSILIAALETAMVSAAKKTFGHQRK
                     IEAQFNPEIGEVELFEAKTVVESVTDA"
     gene            complement(214..720)
                     /gene="rimP"
                     /locus_tag="VF089_08420"
     CDS             complement(214..720)
                     /gene="rimP"
                     /locus_tag="VF089_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX6174025.1"
                     /translation="MGSARFFCFWAMVLDRTVSRIWEVATQIADGEGMEVVDVELRPE
                     GSRRGRVLRLYLDKHGGPNIDELGRVSRQLSELLDTQDIVEGSYTLEVSSPGINRPLK
                     KPEHFQRFIGKRVRVRTGDMIEGRRSFLGILNEVTGEKIKIEVEGKQYEIPFSMIEKS
                     NYEHDWSA"
     gene            773..1957
                     /locus_tag="VF089_08425"
     CDS             773..1957
                     /locus_tag="VF089_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174026.1"
                     /translation="MQAPWAPYCLPNGVYLLCFSRPPIHVMKTIKPSAAAVERQISWE
                     QILSNVEDGVITLDCSGKVSFFNEAAETLTDLAASRALRLSFLQIFRKETWLVELIRK
                     SFPPRQERARGEGDLVSRWGKKVPVGVTVSPLLEPDGSCLGSILLVRNLTHHKELEED
                     LKRADRLAMVGTLAAGLAHEIRNPLGGIKGAAQLLRRSLDHDSSLIEYTNIMNREVDR
                     VNLLIEQLLDLSRPAKLEIVPLNIHQVLEDVLSLELKTHAGPTVVKKLFDPSLPSIRG
                     DRNRLTQVFLNLVKNGYQAMNGSGTLTITTRLDTDRHVRGHGAERSRFIWVEIADQGV
                     GIKDEDLPHIFSPFFTTKNSGTGLGLAVCYWIIKEHGGIIRVESIEGKGSTFKVSLVV
                     AD"
     gene            1959..3404
                     /locus_tag="VF089_08430"
     CDS             1959..3404
                     /locus_tag="VF089_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174027.1"
                     /translation="MDQGKVLVAEDEESLRFILKKALEEEGYWVQTAVDGSGARQSLT
                     DCNFDVALVDIKLPDVDGLTVLKESKDDGVDTAMIIMTAQNTMRNAIGAMKSGAFDYI
                     TKPFDLDEILVLVKRAVDSRKLTRDFRELKEEVKKRFEPGVNIIGTSTSMQRVYKIIG
                     QVVDNPATILIQGESGTGKELVAKTIHYNSPRWNQPFIAINCAAIPRDLLESELFGHE
                     RGAFTGALDRRIGKFELAQGGTLFLDEVGDIPIELQTKLLRVLQDREYSRVGGREVLK
                     ADVRILAATNQDLEKSVHDKRFREDLYFRLKVIPIYLPALRERRGDIPLLIGYFVEKI
                     NREMGIQISGVSPEALRLLEEHPWPGNVRELENTLIRAAVLSAGPILFPKDFNLQNKQ
                     SAAIPEIDQLSLEEVFRHKLEDYFRRTEGVEVDNLYPLVIERIERPLIELTLKKTRGN
                     QIRAAQILGINRNTLRKKITDLRIELKKESS"
     gene            3406..4059
                     /gene="thiE"
                     /locus_tag="VF089_08435"
     CDS             3406..4059
                     /gene="thiE"
                     /locus_tag="VF089_08435"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX6174028.1"
                     /translation="MTLQKAQRTTLPALYAMLDPEQIGSRSPESVLRQLLHGGAKMIQ
                     LRAKAMSSRDFIHLAQTARELTTPFDCKLIVNDRIDIALASAADGVHLGQEDLPLQVG
                     RKLLGDRLIGISTHSLEQAKEAEENGADYIGFGPIFGTVTKNTGYTARGLEMLARIRA
                     AVALPIVAIGGITAANIEDVWQAGADSAAIISDILKSEEVAAKVRAILAAAEPQVRS"
BASE COUNT          987 a         1086 c         1090 g          914 t
ORIGIN      
        1 ggcatcggta acactctcca ccaccgtctt ggcttcgaaa agctccactt cgccaatctc
       61 cggattaaac tgcgcttcga ttttccgctg atgaccgaac gttttcttgg cggcggaaac
      121 catcgcagtt tccaaggcgg cgataaggat cgatttgtcg atcccttttt ctttgcttac
      181 ctgctcgatg acccgattta gatcctgctg catttacgcg ctccaatcgt gctcgtaatt
      241 cgatttttcg atcattgaaa acggaatttc gtactgtttg ccttcgactt caattttaat
      301 tttctcaccg gttacctcat tgagaatgcc taagaaagat cgccgtccct cgatcatatc
      361 accggttcga acccggacac gtttgccgat gaaccgttga aagtgctccg gcttcttgag
      421 cggacggttg atacccggcg aggatacttc cagagtgtaa gatccctcta caatgtcttg
      481 agtgtcgaga agctcactga gttgccggct gacccgcccc aactcatcga tgttgggacc
      541 accgtgctta tcgaggtaaa gccgtagaac tcttccgcgt cggctgcctt cgggccggag
      601 ttcaacgtcg acgacttcca tcccttcgcc gtccgcgatc tgggtcgcaa cttcccatat
      661 tcgcgagacg gttcgatcta gtaccatagc ccagaaacaa aaaaagcggg cagagcccac
      721 ttcgagtcaa actcctaaag tgtgtttttt tctaccacgg aggcgctgat taatgcaagc
      781 cccatgggcg ccctattgct tgcctaacgg ggtttatttg ctatgctttt cacgcccgcc
      841 aatccacgtc atgaaaacaa tcaagccgtc ggccgctgca gttgaacgac aaatctcctg
      901 ggagcagatc ctctccaatg tcgaagatgg ggtgatcacg ctcgattgca gcgggaaagt
      961 ttctttcttt aatgaagcag cggaaacgct aaccgaccta gcggcttcgc gcgctcttag
     1021 gctgtcgttc ctgcagatct ttcgcaaaga aacttggctc gtcgaactga tcagaaagag
     1081 ctttccacca cgacaagaac gcgccagagg cgaaggcgat ctggtgtcac ggtggggcaa
     1141 aaaggtccct gtgggtgtta ccgtttctcc gcttctggaa cccgatggca gctgtcttgg
     1201 gagtattttg ctggtgcgca atctcaccca ccacaaggaa ctggaagaag atctcaaacg
     1261 cgcagaccgg ttggcgatgg ttggtacttt ggccgcgggg ttggcgcatg aaattcgcaa
     1321 tccgctgggc ggtatcaaag gcgccgcgca gctgttacgc cgttctttgg accacgattc
     1381 ttcattgatc gaatacacca acatcatgaa tcgggaggtc gaccgggtta acctgctgat
     1441 tgagcaactg ctcgacctgt ctcgtccggc caaactggag atcgttccac tcaacattca
     1501 ccaggtgcta gaagatgttc tgtcgctgga actgaaaacg catgccggtc cgacggttgt
     1561 caaaaaactt ttcgacccca gtcttccgtc aatccggggt gatcggaatc ggctcacgca
     1621 ggtatttctg aacctggtaa agaatggcta tcaggccatg aacggttcgg gaaccttgac
     1681 gattacgaca cgtctggata cggataggca tgttcgcggc cacggcgccg agcgaagtag
     1741 atttatctgg gtggaaattg cagaccaggg agtcggcatc aaggatgaag acctgccgca
     1801 tattttctcg ccgttcttca caactaaaaa cagcggcact ggccttggac tcgccgtctg
     1861 ctattggatt ataaaagaac acggcggcat tatccgggtg gaaagcatcg aaggaaaagg
     1921 atccactttt aaagtctctc tggtggtagc ggattaacat ggatcaagga aaggttctgg
     1981 tcgcggaaga cgaagagtcg ctgcgcttta tcttaaagaa agcacttgag gaggaaggat
     2041 actgggtcca gaccgcggtg gatggatcgg gcgcgcggca atcgcttacc gactgcaatt
     2101 tcgatgtcgc gcttgtggac ataaagctgc ctgatgtcga cggattgacg gtgctcaagg
     2161 agagcaaaga cgacggcgtc gacacggcga tgattatcat gaccgcgcaa aacactatgc
     2221 gcaatgccat cggcgcgatg aaaagcggcg ccttcgatta catcaccaag ccttttgatc
     2281 tcgacgagat tctggtcctg gttaaacgag cggtagatag ccgcaagctc acccgtgatt
     2341 tccgcgagct caaggaagaa gtcaaaaagc gcttcgagcc gggtgtgaat atcatcggca
     2401 ccagcacgag catgcaacgg gtttacaaaa tcattggcca ggttgtcgat aatccggcaa
     2461 cgatcttgat ccaaggcgaa agcggcacgg gtaaagaact cgttgctaaa acgatccatt
     2521 acaattcgcc gcgttggaat cagccattta tcgcgatcaa ctgcgccgca atcccgcgtg
     2581 atctcttgga gagcgaactc ttcggccatg agcggggcgc atttaccggc gccctcgata
     2641 gacggatcgg caagttcgag ctggcccaag gcggaactct gtttctcgat gaggtcggcg
     2701 atattcccat tgaactccag accaagctcc tgcgcgttct gcaagatcgc gaatacagcc
     2761 gagtgggcgg gcgagaggtg ctaaaagccg acgtccgcat tcttgccgcg accaatcaag
     2821 acctggagaa atcggttcat gacaagcgct tccgagaaga tctttatttc cgcttgaagg
     2881 tgattcccat ttatctgccg gcgttgcggg agcgtcgcgg cgatattccg ctcttgatcg
     2941 gctattttgt cgaaaagata aatcgcgaaa tgggcattca aatttccggt gtttcgcctg
     3001 aagcgttacg gctgctggaa gaacacccat ggcccggcaa cgtccgcgag ttggaaaata
     3061 ccttgattcg cgctgccgta ctgtcggcgg gcccgatttt atttcccaag gacttcaatt
     3121 tacaaaacaa gcagtcggcg gcgattccgg aaatcgatca gctctcgctc gaggaagtct
     3181 ttcgacacaa acttgaggac tactttcgcc gcaccgaagg ggtcgaggta gacaaccttt
     3241 atccgctagt aatcgagcgc atcgagcgcc cactgatcga gctgaccctc aagaaaaccc
     3301 gcggtaatca aattcgcgcc gcccagattc tcggcatcaa tcgaaatacc ttgcgtaaga
     3361 agattactga tctgcgcatc gagttaaaaa aagagagctc ttgatatgac tttgcaaaaa
     3421 gcccagcgga cgacgctacc agcgctgtac gcgatgcttg accccgagca aattggtagt
     3481 cgctcacccg aaagcgttct gcgccagctg ctgcacggag gggccaagat gattcagttg
     3541 cgcgccaaag caatgtcatc cagagacttc atccacctgg cgcaaacggc gcgcgagctg
     3601 actaccccct ttgattgcaa attgatcgtc aatgaccgca tcgatattgc gctggctagc
     3661 gcggccgacg gagttcatct cgggcaggaa gacttgccgc tacaggtcgg cagaaaattg
     3721 ttaggcgaca gactcatcgg gatctccaca cacagcctcg agcaggcgaa agaagcagaa
     3781 gaaaacggcg cagattacat cggcttcgga ccgatatttg gcacggttac gaaaaacacc
     3841 ggctacacgg cgcgcggttt ggagatgctg gcgcggatca gagcggcagt tgctttgcca
     3901 attgtcgcca tcggcggcat tacggcagca aacatcgaag acgtctggca ggccggtgct
     3961 gattcagcgg cgattatcag cgatatctta aaatccgagg aagtggcagc gaaagtacgc
     4021 gcaatcctgg ccgctgctga gccgcaggtg cgctcgtaaa acaacatgag caagagc
//
LOCUS       DASUTG010000368         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1688978, whole genome shotgun sequence.
ACCESSION   DASUTG010000368 DASUTG010000000
VERSION     DASUTG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688978"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF089_08440"
     CDS             <1..530
                     /locus_tag="VF089_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174029.1"
                     /translation="ARRLLKEAAVPEGFSFVLKNRNVKEPYEVSGVFLVDQWRKIGLN
                     VTHTPQEGGPYFNDLRQGNFDAGVDFACDFMDEPDLYLLKYVSTEKSPINYSRYKDAT
                     LDELYERQSRMLDLKDRLPLLRQMERRAIAEQAYQFPLLWWQRIVPHSSRLKGWKIGP
                     SHYVNQDLRDVWLAP"
     gene            570..1523
                     /locus_tag="VF089_08445"
     CDS             570..1523
                     /locus_tag="VF089_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174030.1"
                     /translation="MGQYIVKRLLLMIPTLFGVAVLVFVLLRIAPGDIVELKYAGSGT
                     FAPKEAIERERVQLGLDKPVWQQFLNWSWGIVRLDFGLSMWTGRPIAEEIRIRLELSL
                     QLAIMATVVAILIALPCGTLAALKQDTWIDYLVRIFSIGGLAVPSFWLGIVIILMFLI
                     FFKWLPPLTFTSFWTDPLANMSQMIWPALAVGYRYSAMATRMTRSAVLEVLREDYVRT
                     ARAKGLRENAVVLKHALRNALLPIVTVIGLEFAFLIGGLVVTEQVFNLNGIGMLLVES
                     ITHRDYTMTQALVLLVSFAFIAMNFFLDLLYVWLDPRIHYQ"
     gene            1554..2417
                     /locus_tag="VF089_08450"
     CDS             1554..2417
                     /locus_tag="VF089_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174031.1"
                     /translation="MISFRARRQWQAVRRFAQRRPLGAVGAVIVVLMALAALLAEIIA
                     PFDPLQNNYGAMFHAPGATHWLGTDSFGRDVLSRLLYGSRTALWVGFSSSFFATTLGA
                     VLGVTCAYFGGKVDLVLQRLLDILISFPLIVLAIVVVALLGSGIWNVIVAIAIPMIPR
                     AALVVRSSSLALRQMLFVEAALTLGAGHSRVILRHMLPNVVAPYLIILTAFLGQAILL
                     EASLSFLGLGVAEPAAAWGLMLRGAAVDFAERAPWMALFPGLAISLAVFAFNVFGDSL
                     RDALDPKLRVP"
     gene            complement(2452..>2803)
                     /locus_tag="VF089_08455"
     CDS             complement(2452..>2803)
                     /locus_tag="VF089_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174032.1"
                     /translation="TKYVVPFMEERRSGNIVNLWGGGRAENHGAAPYVVSKDAIRTFT
                     RFIAEEEREWNVCVVAFSPKQAIATEDAPEEARKRLPGPEILGNGFVLAAQAGMELTG
                     KTVEHQNGKLVAVD"
BASE COUNT          505 a          738 c          817 g          743 t
ORIGIN      
        1 aagcgcggcg actgttgaag gaagcggcag tgccggaagg attctcgttt gtcttgaaga
       61 accgcaatgt caaggagccc tacgaagtga gcggtgtatt tctcgtcgat caatggcgca
      121 aaatcggcct caacgtgacg cacacgccgc aggagggtgg cccgtatttt aatgatctgc
      181 ggcaaggaaa cttcgatgcc ggcgtggatt tcgcctgtga cttcatggac gaacccgatc
      241 tttacttgct gaagtatgtg agcactgaga agagcccgat caattactcg cgttacaagg
      301 atgctacgct cgatgagctt tatgaacggc agagccgtat gctggacctg aaggaccgat
      361 tgccattgct acgccagatg gaaagacgcg caatcgccga gcaagcctat cagtttccgc
      421 ttctctggtg gcagcggatc gtgccgcata gcagccggct caaaggatgg aagatcggtc
      481 cgagccatta tgtcaatcag gacctgaggg acgtatggtt ggcgccgtaa gctcctgatg
      541 atcagcggtc atcgtcgatc tcaatttcaa tgggacagta cattgtcaag cgtctgctat
      601 tgatgattcc gacgctcttc ggcgttgcgg tgctggtctt cgtcttactg cgaatcgcgc
      661 cgggagacat cgtcgaactc aagtatgccg gcagcggcac gtttgcgccc aaggaagcga
      721 tcgagcgcga gcgggtgcag ctcggacttg ataaacccgt atggcagcag ttcctgaact
      781 ggtcgtgggg cattgtgcgc ctggattttg gactttccat gtggacgggt cggccgattg
      841 cggaggaaat cagaatacgg cttgagctga gtctccagct ggcgatcatg gcgactgtcg
      901 tagctattct gatcgctctt ccctgcggca ccttggccgc tctgaagcag gatacctgga
      961 tcgactatct ggtacggatc ttttccatcg gcggccttgc cgtgccgtcg ttctggctcg
     1021 gcatcgtcat tattttgatg tttctcatct tttttaaatg gctgccgccc ctgactttca
     1081 cttctttctg gaccgatccg ctggccaata tgtcgcaaat gatatggcct gccttggccg
     1141 tgggataccg ctactcggcc atggcaacgc gcatgacgcg gtcggcggtg ttggaagttt
     1201 tgcgtgaaga ctatgttcgc acagcccgcg ccaaaggact cagggaaaat gcggtcgtct
     1261 tgaaacatgc gctgcgaaac gcgttgttgc cgattgttac cgtaatcggt ttggaattcg
     1321 cttttctgat cggtgggctg gttgtcacgg agcaagtttt caatctcaac ggcatcggca
     1381 tgctgctcgt ggaatcgatc acccatcgag actacacgat gacgcaagct ttggtgctac
     1441 tggtctcgtt tgcgtttatc gcgatgaatt tttttctcga cttgctctat gtctggctcg
     1501 acccgcgaat ccattatcag tgaaacatca cagcgccaga ggaagacgct aggatgattt
     1561 cgttccgagc acgacgacag tggcaggcgg tgcgtcgctt tgctcagagg cggcctctcg
     1621 gagccgttgg cgcggtaatt gttgtcttaa tggctttagc ggccttgctg gcggagatca
     1681 tcgctccttt cgatcctttg cagaacaact atggcgccat gtttcatgct ccgggcgcaa
     1741 ctcattggct gggaaccgat tccttcgggc gcgacgtgct gtcgcggcta ctctatggct
     1801 cccgcaccgc gctatgggtc ggattctctt cttcgttttt tgctaccacg ctaggcgctg
     1861 tcctcggtgt gacatgcgcc tacttcggcg gcaaagttga tcttgttctg cagcggctgc
     1921 tcgacatcct gatatcgttt ccgctgattg ttctggctat tgttgtggtc gcgctgcttg
     1981 gctccgggat ttggaatgtc atagtggcga tcgccattcc gatgattccc cgtgccgctc
     2041 tggtggtgcg gagcagctca ctggcattgc gccagatgct ctttgtcgag gcggcgctta
     2101 ctctaggcgc gggtcacagc cgtgtgatat tgcgtcacat gctgccgaat gtcgtagcgc
     2161 cttatctgat tattctgacc gcctttctcg gccaggcgat ccttcttgaa gcttcgcttt
     2221 ctttcctcgg tttaggcgtg gcggaaccgg cggcggcctg gggattgatg cttcgtggag
     2281 ccgcggtgga tttcgcggag cgcgctccgt ggatggcgtt gtttccaggt ttggccatca
     2341 gcctggcggt gttcgctttt aatgtgttcg gagactcttt gcgtgacgct ctcgatccaa
     2401 aactgcgagt gccgtaagtt tcgcttcatc gttcttttga tttaatgcga cttagtccac
     2461 tgcgaccaat ttcccgttct gatgttcgac agttttgccg gtgagttcca ttccggcttg
     2521 tgcggccagc acaaagccgt ttcccaaaat ctcgggaccc ggtaaacgct tgcgcgcttc
     2581 ttcgggagca tcctcggtgg cgatggcttg tttggggctg aaggctacca cgcagacgtt
     2641 ccactcgcgc tcttcttcgg cgataaagcg tgtaaatgtg cggatcgcgt ctttgctaac
     2701 gacgtagggc gcggcgccgt ggttttcggc tctgccgccg ccccaaagat tgacgatatt
     2761 gccggaacgg cgctcttcca taaaagggac aacgtatttg gta
//
LOCUS       DASUTG010000369         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1540305, whole genome shotgun sequence.
ACCESSION   DASUTG010000369 DASUTG010000000
VERSION     DASUTG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540305"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF089_08460"
     CDS             complement(<1..612)
                     /locus_tag="VF089_08460"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6174033.1"
                     /translation="MSSRLEPATRHYDEKLQHILKTSAKIFAEKGFHRTSVRDISRAT
                     KMSLAGLYYYFTTKEELLYLIQERCFLTLLQRWEEAAGAELDVRARIGLFAANHLSFF
                     WHNTHEMKVMAHEDESLNGEFAGKILVLKRRYVKVLMDLIEEWQQREGGKGVDLRVAT
                     FSLFGMMNWTYTWYQAKRDLPLPQLIEQMLRIFFFGVLKAGQID"
     gene            844..1617
                     /locus_tag="VF089_08465"
     CDS             844..1617
                     /locus_tag="VF089_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007529481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6174034.1"
                     /translation="MDFQNVIVEIDGAIAVVTLNRPQQLNSLSYDLVKDLSLAMQALD
                     QDEAIRVIILTGGEKVFAAGADIKEMAELGPFDERVQGRLAYRDKINKISKPVIAAVS
                     GFALGGGCELAMSCDIIVASETARFGQPEVNLAIIPGSGGTQRLTHLIGKHRAMELVL
                     TGDIIGAAEAKRLGLVNRVMPVELMLEEAKNVARKIAGKPVLAIKAAKEAVLKAANTP
                     LDEGLDFERKSFYMLLASEDRKEGMKAFLEKRKPEYKGR"
     gene            1633..2412
                     /locus_tag="VF089_08470"
     CDS             1633..2412
                     /locus_tag="VF089_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6174035.1"
                     /translation="MAYESIIYEKESGVATITLNRPQALNAFVPQMNQEIMDALKGGE
                     RDPDVRCFMITGAGRAFCAGQDLKGRTPDQKGSLGASLREKYNPMIRQIRQMEKIVIA
                     AVNGVAAGAGCNFALACDLRVASEDAKFIQSFVRVGLAPDSGGSFILPRLVGLSKAME
                     LLLLGETVDAHEAQRIGLVAKVFPAAEFADSARKLAATVAGAPRGIGLIKRAVNHANL
                     PNLESDLEYEACLQEIAGRSSDYDEGVRAFLEKRTPTFTGK"
     gene            2465..>2603
                     /locus_tag="VF089_08475"
     CDS             2465..>2603
                     /locus_tag="VF089_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6174036.1"
                     /translation="MTNPTEKIAVLGAGTMGAGIAQVAAQAGFDTLVYDIKQEFIDTG
                     LG"
BASE COUNT          644 a          696 c          717 g          546 t
ORIGIN      
        1 gtcgatctgt cctgctttta gtaccccgaa gaagaagatc cgcagcatct gctcgattaa
       61 ctgaggcagc ggcaaatcgc gcttagcctg ataccaggta taggtccagt tcatcatgcc
      121 aaaaagagaa aaggtcgcga cccgcaggtc cactcctttg cccccttctc gctgctgcca
      181 ttcttcgatc agatccatga gaactttcac atagcggcgt ttgagcacca gaattttacc
      241 ggcgaactcg ccgttcaagg attcgtcttc gtgcgccatc actttcatct catgcgtgtt
      301 atgccagaaa aagctcaggt gattcgcggc gaaaagcccg atacgagcgc gcacatccaa
      361 ctccgcgccg gcagcttctt cccaccgctg taacagagtc aaaaagcagc gctcctggat
      421 gaggtaaagc agctcctcct tggtcgtaaa gtaataatag aggcccgcta aactcatctt
      481 tgtggcgcgc gagatgtcgc gcacggaggt gcgatgaaag cctttttccg caaagatttt
      541 cgccgacgtc ttgaggatat gctgcagctt ttcatcgtag tgacgggtag caggttccaa
      601 gcggctggac ataaggaacc gatacttaca aatcgccgga gacctgtcaa ttgagccggt
      661 tagcaaccac ccgctcgttc gaaccctgcc cggcccattc accatcattg aaaaagtggt
      721 caaaacttta tcaggagcgg aagaagctat acgcttccgg cacatcacga ttgtcactag
      781 cggcgcggat cgtttcttga atcgaccccg gccatggtgt atgtcttaaa atccaagcta
      841 atcatggatt tccagaacgt tatcgttgaa atcgacggcg ccattgctgt cgtaactttg
      901 aatcggcctc aacagctcaa ttcgctctcg tatgatctgg tcaaagactt gtccttggca
      961 atgcaagccc tggatcagga tgaagccatt cgagtcatca ttctcaccgg tggagaaaaa
     1021 gtattcgccg ccggcgcaga catcaaagag atggccgaac tcgggccgtt tgacgagcgg
     1081 gtgcaaggac gtctcgccta ccgggataag atcaataaga tttcaaagcc ggtgattgcc
     1141 gcggtcagcg gctttgccct aggcggcggt tgtgagttgg cgatgagctg cgacattatc
     1201 gtcgcttcag aaacggcgcg cttcggccaa ccggaagtaa atttggcgat catccctggt
     1261 agcgggggca cgcagcggct aacccattta atcggcaaac accgcgccat ggagttagta
     1321 ctcacgggag acatcatcgg tgccgccgaa gctaagcgtc tgggattggt caaccgggtg
     1381 atgccggtcg agctgatgct cgaagaagca aagaacgttg ccagaaaaat cgccggaaag
     1441 ccggtattgg cgattaaggc agcaaaggag gccgtgctca aagcggccaa caccccgctc
     1501 gacgagggtc tcgacttcga acgaaaatct ttttatatgc tgctggcctc cgaagaccgc
     1561 aaagaaggca tgaaggcgtt tctagaaaag cgcaaacctg aatacaaagg cagataaggc
     1621 gactcacctc aattggctta cgaatcgatc atctacgaaa aagaaagcgg cgttgcgacg
     1681 attacgctca atcgtcctca ggcgctcaat gcctttgtgc cgcaaatgaa tcaggaaata
     1741 atggacgcgc tcaaaggcgg cgaacgcgac ccggacgtgc gttgcttcat gatcaccgga
     1801 gctggtcgcg cgttctgcgc cggacaagat ctgaagggac gaacaccgga tcagaagggg
     1861 tcactgggcg cttcgttacg agaaaaatat aatccgatga tccggcagat ccgccagatg
     1921 gaaaaaatcg tcatcgccgc agtcaacggg gtcgcggccg gcgccggctg caactttgct
     1981 ttggcctgcg acctgcgggt tgcgtcggaa gatgccaagt ttattcaatc gttcgtgcgt
     2041 gtgggcctgg cgccggattc cggcggcagt ttcatcctgc cccggttggt cggcttgagc
     2101 aaagctatgg agctattgct tctgggcgag acagtcgatg ctcacgaggc gcaacggatc
     2161 gggctggtcg caaaagtatt tccggccgct gagtttgccg attcagccag gaaattagcc
     2221 gcgaccgttg ccggcgcgcc gcgcggcatc gggctcatca aaagagcggt gaatcacgcc
     2281 aatttgccaa atcttgaatc cgatctcgag tacgaagcct gcttgcagga aatcgccggg
     2341 cggagctctg actacgatga aggggtacgc gccttcttag aaaaacgcac tccgacattt
     2401 accggaaaat aaagtgattg tgccggtgcg cgcgatcaaa ccggcaactg aattatcaaa
     2461 gcatatgacc aacccaacag aaaaaatcgc agtgctcggc gccggcacca tgggtgcggg
     2521 aatcgcacag gttgcggctc aggcaggatt tgacactctt gtgtatgaca tcaaacagga
     2581 gttcatcgac accggactgg gtc
//
LOCUS       DASUTG010000370         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_651828, whole genome shotgun sequence.
ACCESSION   DASUTG010000370 DASUTG010000000
VERSION     DASUTG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651828"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VF089_08480"
     CDS             <1..296
                     /locus_tag="VF089_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6174037.1"
                     /translation="QVLSKCRRIEGGERVGAVVARGEAELGFQQISELLPIPGVDHVT
                     PLPSEVQKVSVFSAGVARSTADSEAAHALIKFLASPEAAVAITKSGLEPIENR"
     gene            complement(459..1358)
                     /gene="uvsE"
                     /locus_tag="VF089_08485"
     CDS             complement(459..1358)
                     /gene="uvsE"
                     /locus_tag="VF089_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX6174038.1"
                     /translation="MIRIGYACINTKLDPPNRTCRLKNATPEKILELASANVAALQPI
                     LEWNAAHDIEPFRITSDLIPFGSHPINTVPWWRILKSQFVRLGEFIASNHLRVSMHPG
                     QFTILNSPRREVIANSVKELEYHTAVLEALGVDDSHKIVIHLGGIYGDKAESLSRFIE
                     ICKTLASGIKARLVIENDERCYTVTDALIASKAIGVPVVFDVFHHIWNPPLDSVPVRS
                     IIGLAAKTWRKRDGRVKIHYSNQWPGGPAGAHSRSIHLGKFLRFYDSIHDLDIDIMLE
                     VKDKQESVLKIFRALRARQLRAA"
     gene            1735..>2595
                     /locus_tag="VF089_08490"
     CDS             1735..>2595
                     /locus_tag="VF089_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174039.1"
                     /translation="MTLPFFYGWIIVGIAMLAGFLGSGVSNVTMAVALKPITEDLGWS
                     RTLTSSAITIGSVTGGLLAPLIGPLADRLGPRILLPFGAALVGTFVMCLSLTYEPWQF
                     YATFVPARGLAETLLTGVVPMTAVTNWFYVKRPRAIGLVALSVPLGSFVLSLLYQFMI
                     TAYGWRSAFMMLGIALWLLVVLPGLFFLRRQPEDMGVLPDGALPGALKSTLADPESEH
                     ATAAAEHSWTLKDAMRTRTLWLLVAAASFYSISTGGIAFHMVAYFTDVNIAPTLAVAA
                     LSVMALTGAVG"
BASE COUNT          512 a          712 c          737 g          634 t
ORIGIN      
        1 atcaggtgtt gagcaagtgc cggcgcattg aaggcggtga gcgagtcggc gcggtggtcg
       61 cgcggggaga ggcggaactt ggcttccagc agatcagcga actgctgccc atacccggcg
      121 tcgatcatgt gacaccgttg ccgtcggaag tgcagaaagt ctccgtgttc tcggctgggg
      181 tcgcccggag taccgcagat tcggaggcgg ctcacgcgct gatcaagttt ctcgcctcac
      241 ctgaggcggc cgtcgccatc accaagagcg ggttggagcc aatcgaaaat cgataggcga
      301 tctgttcaaa ccgttgcgga cgttaaatcg cttcgctccg ttcaattcgt tcggagaggt
      361 ttgatgaggt cggttgaatt tcggccagcc aaggtctctc ggcacaaggt tgtttctatc
      421 ggattgctta ccgctccggt gtctacctgg ctcaacgatc aagcggcacg aagttggcgc
      481 gcgcgcaggg ctcggaagat cttcaaaacc gattcttgtt tgtctttgac ctcgagcatg
      541 atgtctatat cgagatcgtg gatcgaatca taaaaccgca gaaactttcc gagatggatt
      601 gacctcgagt gagcgccggc gggtcctccg ggccattggt tcgaataatg gattttgacc
      661 cgtccgtcac gcttacgcca ggtcttggcg gcaagtccga taattgatct aactggcacc
      721 gagtcgagcg gggggttcca aatatgatga aagacgtcga atactaccgg cacgccgatc
      781 gctttcgaag cgatgagcgc gtcggtgacg gtgtagcagc gttcatcgtt ctcgatcacc
      841 agccgcgcct taatcccgga agcgagggtt ttgcaaatct cgataaaacg actgagactt
      901 tccgctttgt ctccgtagat gccgcccagg tgtatgacga tcttatggct gtcatcgact
      961 ccaagcgcct cgaggaccgc agtatggtac tcgagctcct tcacgctgtt tgcgatcact
     1021 tcacgacggg gtgagttgag aatcgtaaac tggccggggt gcatcgagac gcgcagatgg
     1081 ttacttgcga tgaactcacc gaggcggacg aattgtgatt tgaggatccg ccaccatggg
     1141 accgtattaa ttggatgcga gccaaatgga atcaggtccg acgtgatgcg aaaaggctcg
     1201 atatcgtgtg ccgcattcca ttccaaaatg ggctggagcg ctgcgacatt ggccgatgcc
     1261 agctcgagta ttttctccgg ggttgcgttc tttaaccggc aggtgcggtt aggcggatcc
     1321 agcttagtgt tgatgcaagc gtagccgata cggatcaaag gacctccttc agtattttga
     1381 ctgcaatccc ttggcggatt agttaaatta acaaaccgat ataaatgatg taaggcggct
     1441 tcaagattcc acacgacgta gcgtacttgg attgtctctt tttttctttt ccgaaagatg
     1501 aaataagaga acaatttggt cgcgcggatc gtcgcaacaa cgatctctcg gaatccgcct
     1561 gtttctagaa cctgaagatg gagtcacaaa cattccttcg caacggagcc agagtgctga
     1621 tgactggcga ttcagtcgct cacatctttt tgtacctggg caaatgctca atgcgccatc
     1681 ggtaagctcc actccgctgg catgctcgcc gaacccattc actgaagccc aaccgtgaca
     1741 ctacccttct tttatggctg gatcattgtc ggcatcgcca tgctcgccgg ctttttggga
     1801 tcgggagtca gtaacgtcac catggcggtg gctctcaagc ccatcaccga ggatcttggc
     1861 tggagccgca cactcacatc ctccgcgatc accattggat cggttactgg cggccttttg
     1921 gcgcctttga tcggtcccct cgccgatcga ctgggtccgc gcatcctgct tcctttcggc
     1981 gccgctttgg tcggcacatt cgtcatgtgc ctgagcctga cctacgagcc ctggcagttc
     2041 tacgcgacgt ttgtccctgc ccggggattg gctgaaacat tgctcaccgg tgtcgtgccg
     2101 atgactgcgg tgaccaactg gttttacgta aagcgtcccc gcgccatcgg tttggtcgcg
     2161 ctttctgtgc cgttgggatc ttttgtctta tctctgctct atcaatttat gattacagcc
     2221 tacggctggc gtagcgcgtt tatgatgctg ggaattgcgc tctggctgct ggtggtattg
     2281 cccggcttgt tttttctacg tcgccagccg gaggacatgg gagtgctgcc cgacggagcc
     2341 ttgccgggcg ctttaaagtc cacgctagcc gatccagaat cggagcacgc gaccgcggcc
     2401 gctgagcata gctggacgtt gaaagatgcc atgcgcaccc gcacgctctg gctcctagtt
     2461 gccgccgcca gcttctattc aatttccacc ggcggcatcg cttttcacat ggtggcgtat
     2521 ttcaccgacg taaacatcgc gcccaccctg gccgtggccg cgcttagtgt gatggcgctg
     2581 accggcgccg tcggc
//
LOCUS       DASUTG010000371         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1838680, whole genome shotgun sequence.
ACCESSION   DASUTG010000371 DASUTG010000000
VERSION     DASUTG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838680"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /locus_tag="VF089_08495"
     CDS             complement(<1..74)
                     /locus_tag="VF089_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004873757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX6174040.1"
                     /translation="MSVVAIIPARYGSTRLPGKPLAWIG"
     gene            360..1298
                     /locus_tag="VF089_08500"
     CDS             360..1298
                     /locus_tag="VF089_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174041.1"
                     /translation="MAELIKIAAGFATIGQGAGPMMVTWKAGLFKKHGLDIDKPRIMG
                     GAKGVVRGLMTGEIQFGNMAAPAPLRSNVKGEADLIFLTGGINQQFVMTRPGITGREQ
                     LTGKKIGFVGDGGLNDALVSFIIEKLSEAGIEGVEKEPVPPGGDRQIAALVSGSCDAI
                     VITPPESIEARRRGCNYLIDFAEYGLNYALGGIAARREYVEKNPEITGRFIKAYVEGM
                     QRYRTDRDFTVNVQAEYSGIADRTVAEETYDLTRPGMPLIPYPVVASLQVLLDFMSKE
                     VLEAKNVDARRFVDSRFISELEQSGFIASLSGQRAQ"
     gene            1523..1759
                     /locus_tag="VF089_08505"
     CDS             1523..1759
                     /locus_tag="VF089_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174042.1"
                     /translation="MHVQSYRAGKIKLAQRMVTEARAGKHQFDVLLSSAFTTLEMKLQ
                     KLLAKYESPERAHYRAAAPRLFLLVWCCTPPTSY"
     gene            1818..2840
                     /locus_tag="VF089_08510"
     CDS             1818..2840
                     /locus_tag="VF089_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174043.1"
                     /translation="MNGFVHARLPLALLTAAFIYFGRYDFSLAAGTDALIEAAKRESE
                     VVVYASMNLAEASALIGEFEKRYPFIKVKLNRSGSEKLLTRLLTEARAKKTFADVIQT
                     VEFSMHIFSRQGVLGRYIPQANALYPKEFKEEGYWTTVYYNPYVVGYNTRLVPDRMVP
                     RTYDDLLDRRWKGKIMMEGTKADWFAGMLQIMGQERGLKYMRELAKQQPSLREGHELL
                     AQLVAAGEGALDINIPAASVERNKEKGAPIDWTGMEVAPAVMVGIGLSAQAPHPNAAK
                     LYIEFVLSREGQKLMQTPGRLIARSDLAVEQSAMFKEIKMVPVNPALAEKLGEYSKQL
                     RAIFGG"
     gene            2973..3743
                     /locus_tag="VF089_08515"
     CDS             2973..3743
                     /locus_tag="VF089_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX6174044.1"
                     /translation="MRMMFLNHSFKRHSAELETHIQRLLKSYASPETTFELAYPDDLG
                     GGEVLSLLEQGKALSGLHHILETPALVKKAIEAEQLQFDAVIQSNTFDPGVEASRLAV
                     RIPVIGLLRASLHFAATICDRFGLIVPLPAHIPHTMRLVQSYGMTRFVSGIKTVNLYD
                     TGDLSGYHDLIIERSLAVGQELVEQGAQAIIPLGGKIYPYVVTPEELAPSLGVPIINT
                     KAVGVSFAELMVRSRTTHSQLAYPWSPGLNPDKVSRRQ"
BASE COUNT          977 a         1029 c         1076 g          854 t
ORIGIN      
        1 ccgatccagg ctaacggctt tcccggaagc cgggtcgaac catagcgcgc cgggattatt
       61 gccacgactg acataaacgt gcgcttcaga ggtgcgatga ttgcgtaaga aaggtcaaag
      121 ttgcgttctc gtcgtcgaaa taaaaaaccg caatggcttt cggcgaaagc gcatcacgga
      181 attcaataat acgcccaggc caaaagggtg tcaatgtgat ttgctgcaac caaccgtgcc
      241 ctacaacata tccgcctcca ctcgcatgag tgtcaatcgc cgagaaggac aggcaattca
      301 tcttgactta tcgatgccat cctatgatag gagtggtgcg gtaagaggag acgagcggca
      361 tggcggagtt gataaaaatc gctgccggat ttgccacgat tggacagggc gccggcccga
      421 tgatggtaac ttggaaggcg ggcttattta agaagcacgg cctcgatatc gacaagcccc
      481 gtatcatggg cggcgccaaa ggagtcgtgc gcggcctaat gaccggtgaa attcagttcg
      541 gcaatatggc agcgccggcg ccgttgagat ccaacgtcaa aggcgaagcg gatttgatct
      601 ttttgaccgg cggcatcaat cagcaattcg tcatgaccag gccgggaata actggccgcg
      661 agcagctcac cggaaaaaag attggttttg tcggcgacgg cggtctcaat gacgcgctgg
      721 ttagcttcat cattgaaaaa ctgtcggagg cgggaatcga aggcgtcgag aaagaacctg
      781 ttccgcccgg cggcgatcgg caaatcgctg cgctggtcag cggaagctgc gatgctatcg
      841 tgattacccc gccggaatcg attgaggccc gccgaagagg ctgtaactac ctgatcgatt
      901 tcgccgaata tggtcttaat tatgcgctgg gtggcattgc cgcgcgccgt gaatatgtgg
      961 agaaaaatcc cgagatcaca ggcaggttta tcaaagcata cgttgagggg atgcagcgct
     1021 accggaccga ccgcgatttt acggttaacg ttcaggctga atatagcggc attgcggatc
     1081 gaacagtcgc ggaagaaacc tacgatctca cccgcccggg tatgccactg attccttatc
     1141 cggttgttgc ttcgctgcaa gttttgctcg atttcatgtc caaggaagtt ctcgaggcga
     1201 agaatgtcga tgcgcgccgc tttgtcgata gcaggtttat ttctgaactc gaacagagcg
     1261 ggtttatcgc gtcactgtca ggccaaagag cgcaatgata ggagtcgtaa aatgaataaa
     1321 aaacatttcc ggcgcccgaa tcatcgagtt ttggcattaa tgactttggc catgctctgg
     1381 ataaacaccg acaacctcca ggcagccggc cacatcaaca tggaagccgc gcgtaaggaa
     1441 gcccgggtca ccctctactc gtcaatctcg cttgaagata taaacattct gacgcgagcc
     1501 cttcgagaaa aaacatcctg gcatgcacgt gcaaagctat cgcgcgggca agatcaagct
     1561 ggcccaacgc atggtgaccg aggcgcgcgc cggcaaacat cagtttgacg tgctgctttc
     1621 cagtgccttt acgacgctcg aaatgaaatt gcagaaactg ctcgccaaat atgagtcgcc
     1681 ggaaagggcc cattatcgcg ctgcggcacc tcgtcttttc ttactagttt ggtgttgtac
     1741 gccgccgaca tcgtattgat ttgacccgcg gtccaaaaac cttctgggtc cttcaactcg
     1801 gcagaaagga aggcgtgatg aatggatttg tccacgctcg gttgccgttg gcgcttctta
     1861 cggcggcatt catttatttt ggcagatacg atttttcact cgcggcgggt acggatgctc
     1921 tcattgaggc ggccaagcgc gaaagcgagg tagttgttta cgcgtcgatg aatctggccg
     1981 aagctagcgc tttgatcggc gagttcgaga aacgctatcc attcatcaaa gttaagctca
     2041 accgcagcgg tagtgaaaaa ttactgaccc ggctccttac ggaagcgagg gcgaagaaga
     2101 cgtttgccga cgtgattcag acggtcgagt tcagcatgca tatttttagc cggcagggcg
     2161 tgctggggcg gtatatcccc caagctaatg cgctctatcc gaaagaattc aaagaagaag
     2221 gatactggac aacggtttac tacaatccgt atgtcgtcgg atataacacg agattagtgc
     2281 cagatcgtat ggttcctcga acatatgacg acctgcttga ccgacgatgg aagggaaaga
     2341 tcatgatgga ggggaccaag gcggactggt tcgccggcat gctgcaaatc atgggacagg
     2401 agcgcggcct aaaatacatg cgcgagctgg ctaaacagca acccagcctg cgcgagggac
     2461 atgaactatt ggcgcaactc gtcgccgccg gagaaggcgc tctcgacatc aatatccccg
     2521 ccgcatcggt ggaacggaac aaggaaaaag gcgcgcccat cgactggact ggcatggagg
     2581 tggctccggc cgtcatggtg ggcatcggtt tatcagcgca agcgccgcat cctaatgcag
     2641 ccaagctcta catcgagttt gtgctttctc gggaaggcca aaagctgatg caaacgccgg
     2701 ggcgactgat cgcccggagc gatcttgcag tggagcagtc cgccatgttc aaagaaatca
     2761 aaatggtgcc ggtcaatccg gccttggcgg aaaagctcgg cgaatactcc aaacaactca
     2821 gagcgatttt tggcggttga gcagttagca ttgccaatgg cggatttgag attttcaatt
     2881 ttcgggcttt gccgcagacc tccgacagtt tcattcgatt tgccgaatct aagaacgaga
     2941 gcgacaacgc tttctttgca aaggagtaag ccatgcgcat gatgtttctg aaccactcgt
     3001 tcaagcgtca ctctgccgag ctcgaaactc acatccagcg gcttctgaaa agctatgcct
     3061 cgccggagac caccttcgaa ctcgcctatc cggatgatct tggcggcggc gaagtactga
     3121 gcctgctcga gcaaggaaaa gcattatccg gcctgcacca cattcttgaa acacctgctc
     3181 tggtcaaaaa ggctatcgaa gccgagcaat tacaattcga tgcggttatc cagagcaaca
     3241 ctttcgatcc cggcgtagaa gccagccggc ttgcggtgcg catacctgtc atcggtctac
     3301 tgcgcgcgtc tttacatttt gcagcgacta tttgcgaccg cttcggcctg atcgtaccgc
     3361 tgccggcgca catccctcac actatgagat tggtgcaatc ctacggcatg actcgtttcg
     3421 taagcggcat caaaaccgtg aacctctatg acaccggtga cttgtccggg tatcatgatc
     3481 tcatcatcga acggagcctc gccgtcggcc aggagcttgt tgaacaagga gcccaagcca
     3541 ttattccact cgggggcaag atttacccgt acgttgtaac accagaagag ctagcgccca
     3601 gtcttggtgt gcccatcatc aataccaaag ccgtgggcgt aagctttgcc gaactgatgg
     3661 tcagatctcg aactacacac agccaacttg cctatccatg gtcgcctggt ttaaatcccg
     3721 ataaagtgtc taggcgccaa tgaatagccg cttcaagtga tcgataggcg aagctcgttg
     3781 ccgctgcagg tagttaacaa tccacacgaa cggagacgcc ttccgtgaaa tcccgacacc
     3841 aacatccgca aagcactcat cccatcacat agcggcgggc gagggaggac aaattgtgat
     3901 tatcgatgct gatgggcatt tttttgagac cgaaga
//
LOCUS       DASUTG010000372         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_355377, whole genome shotgun sequence.
ACCESSION   DASUTG010000372 DASUTG010000000
VERSION     DASUTG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355377"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VF089_08520"
     CDS             complement(<1..185)
                     /locus_tag="VF089_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174045.1"
                     /translation="MENRNIRLATSLILWAWFSLYLTAPLMGAKIDCSPVTNTSNELP
                     RTAAIGTNPAGTGAHSLG"
     gene            complement(390..1487)
                     /locus_tag="VF089_08525"
     CDS             complement(390..1487)
                     /locus_tag="VF089_08525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174046.1"
                     /translation="MEQNLIDADGHVLEDETLMDFLEEPYRSCKTQGGIIGPIRNVLG
                     NVFPTLDFLHLATFKRSERAFGGGKRVGPEQWLEFIDVAKFEYAFLYPTVGLAIGNIV
                     HPDWACVVARAYNNWIHDRYMKRSPRLKGIALLPMQDVSEAVNELRRAVKELGMLGAM
                     LPSRGLPFDLGHRTYWPIYAEAEKLGCALAVHGGCHHGMGLDTFETFVPIHGLGHPLS
                     LIIAFSGMVYHGVFDEYPRLRVGFLEGGAAWATFWMDRMDRSHHYFGELNIQGRYKGP
                     SMQQKPSEYLKKDNVFIGCEGGEEGLDYQIRRTGNSHFLFASDFPHEIGPEDIMHEIE
                     EVQEYPGLNDEDKAAILGANARRFYQIPQGQ"
     gene            complement(1788..>2126)
                     /locus_tag="VF089_08530"
     CDS             complement(1788..>2126)
                     /locus_tag="VF089_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174047.1"
                     /translation="YGSMETFLANAKRVLRQNGYFIYADFRNIDDVAVLHGLMEQSGM
                     KILKREDITANVLRSLDLDDRRRVLLIRALFRNWLLNPFLEFAGVKGSKIYSAFQSGK
                     MIYLHYVLQK"
BASE COUNT          519 a          603 c          517 g          487 t
ORIGIN      
        1 ccgagcgagt gcgctccagt cccggcaggg ttggttccta tagcggccgt gcgcggaagc
       61 tcgttactgg tattggtgac gggactacaa tcgatcttgg ctcccatcag cggggcagta
      121 agatacagcg aaaaccatgc ccacaaaatc aacgatgttg ccaagcgaat gtttcgattt
      181 tccatacgcc ctccctattc ctcaagtgcg gttttctcga tcgattgagt ctctttcgat
      241 ccgtacatgc gctgccataa atccgcatcc ctacagttct caagcagaca cagccttatc
      301 tcagcatgac ttcttgacag gatgaccggc agcaagcgcg cgagcggcca agaagcgaac
      361 tccaccgtga ccttttatgg atcgcacttt tattgccctt gaggaatctg gtaaaagcgt
      421 cttgcgttag ctccgagaat ggccgctttg tcctcgtcat tgagacccgg gtattcctgc
      481 acttcttcaa tttcgtgcat gatgtcttca ggcccgatct cgtgaggaaa gtcgctggca
      541 aaaaggaagt gggagtttcc cgtccgcctg atttggtaat ccagtccctc ctcaccaccc
      601 tcacagccga tgaaaacatt atctttcttg agatactcgc tgggtttttg ctgcatagac
      661 ggccccttgt atctcccctg gatattcagc tcgccaaagt agtgatgcga ccggtccatt
      721 cgatccatcc agaaagtcgc ccaggcggcg ccaccctcga gaaaaccgac ccgcagccgc
      781 ggatattcat cgaacacgcc gtgatagacc atacccgaga aggcgatgat caaggaaagc
      841 gggtggccca gcccatggat cggcacgaag gtctcaaagg tgtcaagccc catgccgtga
      901 tgacagccgc cgtggacggc aagcgcgcaa cccaacttct cggcttctgc atagatcggc
      961 caataggtcc tatgccctag atcgaaaggc aagccgcggg agggtagcat cgcccccagc
     1021 atgccaagct ctttgaccgc gcggcgcaac tcgttgaccg cctcgctcac gtcctgcata
     1081 gggaggagtg cgatcccttt gagacgcggg ctgcgcttca tgtagcggtc gtgaatccag
     1141 ttgttgtaag cccgcgcaac tacgcaagcc caatccggat ggacgatatt gccgatggcg
     1201 agtccgacgg tcgggtaaag aaacgcatat tcaaacttcg ccacatcgat aaattccagc
     1261 cactgttccg gaccgactcg cttaccgcca ccgaaagccc gctcgctgcg cttgaaggtg
     1321 gccaggtgaa ggaaatccag agtcggaaat acattgccta aaacatttcg tatcggtccg
     1381 atgattccac cctgggtttt gcagctgcga taaggttcct ccaggaaatc catgagagtc
     1441 tcgtcttcga gcacgtgacc atcagcatcg atgagattct gttccatgtg catctccaaa
     1501 cgttctgtcc ctgtgactgt acaagtcccg accgcggtag gccttaaatc tttgcctcaa
     1561 cggtacgacg aacagcaatt ccgtgcaaat tcaaaacgga aggggcgccg ggatcaatga
     1621 cgtacttact attccgctga aatactctca gaaggagacg aagctgctca acgcagactg
     1681 ctcaaagatc ataggcgaag cccgcggaaa atccactagc ggcgtgctcg aactacaact
     1741 tccccggaga gtgatatgcc acggtttctc gttttgtgcc ctgccggtta tttctgcaga
     1801 acataatgca aataaatcat cttgccactc tggaacgcgg aatagatctt cgatcctttc
     1861 acgccggcga attctagaaa tgggttaagt agccaatttc gaaacaaagc tcgaatcagt
     1921 agaactcttc gtcgatcgtc cagatccaac gaccggagaa cgttcgcagt aatatcttcc
     1981 cgtttgagaa ttttcatgcc tgactgctcc atcagaccgt gtaagaccgc aacatcatct
     2041 atgttgcgaa aatcagcgta gataaaatat ccattttgtc gaagcactct cttcgcattt
     2101 gcaagaaaag tttccatcga accata
//
LOCUS       DASUTG010000373         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1689702, whole genome shotgun sequence.
ACCESSION   DASUTG010000373 DASUTG010000000
VERSION     DASUTG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689702"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..887)
                     /locus_tag="VF089_08535"
     CDS             complement(81..887)
                     /locus_tag="VF089_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174048.1"
                     /translation="MRYLSRRLRLFLSIFLILCFSLLEHVFADDVVLMSYGGHNETIG
                     PYWVAVDKGLYQKHGIDARLLQVRNAQISLTALISGEVPIFLPSIANVLSGITAGAKL
                     GCIAFPIKGVSRELVVRRDIDSLPALRGKTIGVQSIGGGFWLQTMMLLDAVGVDPDKI
                     GMKMRVIGEEATIVQALVSGNVDAAVVTLPSAQVAKRGGARTLVSTTDLNIALQTVGL
                     CGRNERIANSPELMTRLARGMVDGIVYILDPRNKREVSAVLKKHLRLSAP"
     gene            complement(1181..2368)
                     /locus_tag="VF089_08540"
     CDS             complement(1181..2368)
                     /locus_tag="VF089_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX6174049.1"
                     /translation="MTEVLHSAENHNGSAPLASSNIRHLCRMELAGGGQIVIDGNYAY
                     VGHQHRPNGTTILDISDPRKPKILSTLTPNHPWSHSHKARIVGDLMVVNSEFERGDGR
                     LSEYPDGGFRLYDVKDRTKPKLVSFVKTYGKGVHRFDIDEKYAYISTEMEGYIGNILV
                     IYDIRNPSRPVEVSRWSMERQNVAGGDEPHPKRAQHRLHHALRCGTEMYAGCWMSGIS
                     IIDVTNVNAPRTLSRYEYDPPCPEPTHTFLKAPHPIGGKRIAVSTEEERSQRGADAGK
                     PHAPLRTWDVSDPTHPKLLCSYYLPESATPYPAGKVRFGTHQLREKVDEDNLLYVTWF
                     AGGLRIIDISDPANPLERGYFIPKPGEGVAAPLTNDIFKDDRGLLWVTDKERGLDVIE
                     YEA"
     gene            2796..3770
                     /locus_tag="VF089_08545"
     CDS             2796..3770
                     /locus_tag="VF089_08545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX6174050.1"
                     /translation="MLDNFLQILPEIIFDAAEKLGGRCTGRFYALNALENRVYDIEME
                     EGPHVIIKFYRPGRWSRATIQSEHDFLKALEESEIPVVCPLTDDQGQSIFEINGVMFT
                     IFPKRPGRLEPELTAEQLTRLGRFMARLHNVGATVKAAPRLRLDPQTYGREPLEFLKE
                     GNFVPMELASRFETIVAQVCDAITPRFEDVHYVLLHGDCHSGNILWNRDNPYFIDFDD
                     MLYAPPVQDIWMLTGADDEYGKEQRRILLDAYQQIRTFDMTTLQLIEPLRALRMIHFS
                     AWIARRWEDSAFKVAFPDFGTEHYWQEQIEALALQLEKVQYHTMDLYR"
BASE COUNT          910 a         1149 c         1115 g          898 t
ORIGIN      
        1 cgtttttaaa cgccgcaaga tccggcgcaa tgtcgagact gattatagtt ctgagcgaat
       61 gataggaggt ctccgcgact tcatggggcg ctcagtcgta ggtgcttctt caaaaccgcg
      121 ctaacctctc ttttgtttcg cggatcaaga atataaacaa taccatcaac catgcctctg
      181 gcgagccggg tcatcagctc cggcgagttg gcgatacgct cgttgcggcc gcatagcccg
      241 acggtttgca gtgcgatatt caaatccgta gtgctgacta aagtgcgtgc gccgccacgc
      301 ttggcgacct gcgcggaagg caaagtaacc accgccgcat cgacgttgcc gcttaccaaa
      361 gcttgcacaa tagtcgcctc ctcgccaatc acgcgcattt tcatgccgat cttatcgggg
      421 tcaacgccca ccgcgtcgag cagcatcatg gtttgcagcc aaaaaccgcc gccaatactt
      481 tgcacgccaa tagtcttgcc gcgcagcgcc ggcagcgaat cgatgtcccg gcgcaccacc
      541 aattcccgcg aaacgccttt gatcggaaaa gcgatgcagc cgagcttggc gccagcggtg
      601 ataccgctca gtacgttggc aatagaaggc agaaatatcg ggacctcgcc ggatatcagg
      661 gcggttaaac tgatctgggc attgcgcact tgcaacaagc gcgcgtcgat accatgtttc
      721 tgataaagcc ctttatcgac ggcaacccag tatgggccga tggtttcgtt gtgcccacca
      781 taagacataa ggacaacatc atcggcgaag acatgctcga gaagcgagaa acagagaatc
      841 aggaaaatcg aaagaaagag tctcaaacgt cgagaaagat aacgcatgca tcacctccga
      901 cggttcgccg atatgccaat accctatcgc acgatgatcg ttaatctctc gcaccgaaga
      961 ccgtgagccc ttacgggttt aaggcataca ccttgagcta taccgctcac tctactgcgc
     1021 caaccgctca aggtaatact gccgatccga atcactgcaa aaaaataccc gccgttgatt
     1081 gctccggcag ctcacatggt aaacgcctcg gcgaatttga agctgcctcg attcgcgggt
     1141 caagacgcga cagcatttac ttaatgtgcc gggctccagc tcatgcttca tattcgatga
     1201 cgtcgaggcc gcgctccttg tctgtcaccc acagaagacc gcgatcgtct ttgaagatgt
     1261 cgttggtcag cggcgccgcc actccctccc ctggtttcgg aatgaaatag ccccgctcca
     1321 agggattcgc cggatcgctg atatcgatga ttcggaggcc gccggcgaac caggtaacat
     1381 agagaagatt gtcctcgtcc accttctcgc gtagctgatg cgtaccgaag cggaccttgc
     1441 cggcgggata tggcgtcgcg gactccggaa ggtagtacga gcacagcagc ttcggatggg
     1501 tcggatcgct gacatcccat gtgcgcaacg gcgcgtgcgg cttgcctgca tcggcgcccc
     1561 gctgcgagcg ttcctcctct gtggatactg cgatgcgctt gccgccgatc gggtgcggcg
     1621 ctttgaggaa ggtgtgggtc ggctcgggac aaggcgggtc atactcatag cgcgacagcg
     1681 tgcgtggagc gttgacgttg gtcacgtcga tgatcgaaat gcccgacatc cagcagccgg
     1741 cgtacatctc ggtgccgcag cgcagcgcat ggtgcaggcg gtgctgcgcc cgcttcgggt
     1801 gcggctcgtc gccgcccgcc acgttctgtc gctccatcga ccagcgcgag acttccaccg
     1861 gcctcgatgg attgcggata tcgtagatga ccagaatgtt gccgatgtag ccttccatct
     1921 cggtcgagat gtaggcgtat ttctcgtcga tatcgaaacg atgcacaccc ttgccgtacg
     1981 tctttacgaa gctcacgagc ttgggcttgg ttcggtcttt gacgtcatag aggcgaaaac
     2041 cgccgtccgg atattccgag aggcggccgt cgcccctctc aaactcggaa ttgacaacca
     2101 tcaggtcgcc gacgatccgc gccttgtgcg agtgcgacca gggatgattg ggcgtgagag
     2161 tgctgagaat tttcggcttg cgcggatcgg aaatatccag gatcgtcgtt ccattcggcc
     2221 ggtgttgatg gccgacatag gcataatttc cgtcaatgac gatctgccct ccccccgcaa
     2281 gctccatccg gcagagatgg cgaatgttcg acgatgcgag cggcgcactg ccgttatgat
     2341 tttcagcgga atgcaaaact tcggtcatgt tgcacctttc tggcgaattt gccggcgatt
     2401 atcgccggtg ggataatact tgacttcgaa ccaatggcac actcggcatc ctctcattct
     2461 cactgttgac tctggaaccg gcggttgccg atcgtcgcct gttgcctcgt gcctgttcct
     2521 gttaaaccgc gcctattttg cacactttca ttggtgaatc aaaagcatgc gccgatccga
     2581 accggcactt tgttgagacc cagcatttct tggcgtcgaa aacccagtgc aggtaaccgc
     2641 tacggttgcg tttcgaaaca agtcgatttt tcgcccgttc ctttaatcaa gggctttaac
     2701 ctagcgcggc gattgaacgg cttgaacgtt ttgaacaact tttcgctgga taacgggagt
     2761 gtacaaggag aggacgctcg tctaggatga aggctatgct agataacttc ctgcagatcc
     2821 ttccagaaat catcttcgac gcggccgaga aattgggcgg gcgttgcacg gggcgcttct
     2881 atgcgctcaa tgcgctggaa aaccgtgtgt atgacatcga gatggaagaa ggcccgcacg
     2941 tcatcattaa attttatcgc cccggccgct ggagccgcgc cacgattcag tccgagcacg
     3001 attttttgaa agcccttgaa gaaagcgaaa ttcccgtcgt ctgcccgctc acggacgatc
     3061 aaggtcaaag tattttcgaa attaacggag tgatgttcac catcttcccg aagcgtccgg
     3121 gccggctcga gccggaactc accgccgaac agctcacgcg attgggaagg ttcatggctc
     3181 gccttcataa cgtcggggcc acggtaaagg ccgctccccg attacggctc gatccgcaaa
     3241 cttatgggcg cgagccgttg gagtttctaa aagaaggcaa cttcgttccg atggagctag
     3301 ccagtcgatt cgagaccatc gtagcgcagg tttgcgacgc cattactcct cgattcgagg
     3361 acgtccacta cgttctgctc catggcgatt gccattccgg gaatattttg tggaatagag
     3421 acaatcccta cttcatcgac ttcgatgaca tgctctacgc gcctccggtg caagatatct
     3481 ggatgctcac cggcgcggac gacgaatacg ggaaggagca aaggcgtatc ttactcgatg
     3541 cctaccaaca gatcagaact ttcgacatga cgacgttgca gctgatcgag ccgctgcggg
     3601 cgctgcgcat gattcacttt tccgcttgga tcgcgcgacg ctgggaagac agcgctttca
     3661 aagtagcgtt tcctgatttc ggcaccgagc actattggca ggagcaaatc gaagcgctag
     3721 cactgcaatt ggaaaaagtg caataccaca cgatggatct gtatcgttga aaattcttaa
     3781 accagtgttc gccaaacggc ttgaacggag cggagcggtc gaaggattgg aaaagtttgg
     3841 aatagtccga gcccgtgata aacggacctc ggactatcca gtagattgaa actctggagc
     3901 ttccggaacg tgtggcacca catggttcta acatcttgaa cggagcgcag cggctgcact
     3961 tggaacgcgc ctcgagtgct caggcctcgg tgtaccaggt cgtatggcgc tcaacaggca
     4021 ggcgcttggc catcgtcttc tgctcggagt atcccagaaa tatacatgcc ac
//
LOCUS       DASUTG010000374         7806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1394012, whole genome shotgun sequence.
ACCESSION   DASUTG010000374 DASUTG010000000
VERSION     DASUTG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394012"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..302
                     /locus_tag="VF089_08550"
     CDS             75..302
                     /locus_tag="VF089_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174051.1"
                     /translation="MNSKPYKNYFIDSESFQREKHGSWVPQYTIARRVTESNDFPSHQ
                     YQFSHTFPTKNEADCFAVEKAQEWIDKNQKR"
     gene            complement(557..1096)
                     /locus_tag="VF089_08555"
     CDS             complement(557..1096)
                     /locus_tag="VF089_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174052.1"
                     /translation="MNPLLIIAIPLALVILGMILNSTGLSYDQPASSPEQDPLKKLTA
                     EKESYRKFFDLQRSRSLKRQKRVSQYSWLVMAAFIGAFIGLYTDTVNKTSLSSRIAAI
                     QTLGTEEGKQMVLSVTLSDGNNVKYLVKLPQAEKLETAPKEGMAVEKVSSWELERLGT
                     ALSVGNSPLPLGMALKISH"
     gene            complement(1725..2642)
                     /locus_tag="VF089_08560"
     CDS             complement(1725..2642)
                     /locus_tag="VF089_08560"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6174053.1"
                     /translation="MPSDRKIEGILMPMPVAFKNDGAIDHSATDAIIDFYLSSGVHGF
                     FPLGTHGQGMVMEIDERKKVAEQIVKRVAGKVPVVMHIGTANTYSSIELAKHAASLGV
                     DAIGVVPPYYYPHDDYEVYAHYKAIAKSVPGVPLFIYDNTETTWVEITPAKANKIIEA
                     IAPNPLAGIKVSFIAYDKLLGYVYQLPKSTGVFPGGIFSLYTGYSVGVRGAIHPPSTP
                     FPEACVKMYEALKLGDLKGAQKEHDLLTRLMQVVGKYLKTHGRGVFCELMKLRGLQVE
                     RFPRWETMPFSEKDKAELKEGLNNAGFKL"
     gene            2779..3291
                     /locus_tag="VF089_08565"
     CDS             2779..3291
                     /locus_tag="VF089_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6174054.1"
                     /translation="MAEKNPTVTVSTSMGDIRIELESEKAPITTKNFLDYVSDGFYDG
                     LIFHRVIPGFMIQGGGMDPQMKQKSTKSPIKNEAANGLKNKNGTVAMARTNVVDSATA
                     QFFINVKDNDFLDHRSTSADAFGYAVFARVIDGMDVVQKIEKVKTRNSGMHQDVPVDP
                     VVINSVRVDG"
     gene            complement(3466..4221)
                     /locus_tag="VF089_08570"
     CDS             complement(3466..4221)
                     /locus_tag="VF089_08570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6174055.1"
                     /translation="MNYTLRELMVAAASREIRDGDKVFVGMRLPLLGYAVAKETHAPS
                     ALGIFENGVIRDWPALESILTMSDPPNVARALSCTGLNDVMHLLQSNRIDLGFIGGAE
                     VDRFGNLNTHWVEENGKRTRLPGSGGAADIAAMAKRCIIIMNHERRRFPARVRYITSP
                     GFGDGGDWRSRNGLIGGGPRRVITSMGIFSFDSNTHEMMLTANHPGVSIENIKSETGW
                     PLEISPDVKETAAPTDDELSAIRKYDPKGVWTS"
     gene            complement(4359..4796)
                     /locus_tag="VF089_08575"
     CDS             complement(4359..4796)
                     /locus_tag="VF089_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6174056.1"
                     /translation="MKAVPFFKLTQEGADIIVNAAIEKAKELKQIVSIAVVDSGGHLF
                     SFKRMTNGRTHNTMIAVAKARSAALTTAATGKTNREGGEIPDHQVLAQTLAVGPGWFI
                     SIEGGIPLTIEGQCIAAVGVSGAPAAVDKQICQAGVDAFNKAA"
     gene            complement(4827..5738)
                     /locus_tag="VF089_08580"
     CDS             complement(4827..5738)
                     /locus_tag="VF089_08580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6174057.1"
                     /translation="MDKVRSLHDAIAQDIDDGNSIAMGCALESLIPFAASYEIIRQRK
                     KNLTLIAPISDMQFDQLIGAGCVKKIIASWVGNVAAGLGHNYRRAAESAIPHPIEIEE
                     HSNFTIGLGLQAAAIGLPFLPTKSIRGSDISHSPQFGSVKCPFTGDELLAVKAITPDV
                     AILHVQRADRDGNAHAWGNFGVIKEAAFAAKKVILTCEEIIDHDVILSDPNRTLIPGF
                     LVASVVHQPFGSHPSPAQGYTRRDDSFYFEYHRETRSRDGFEKWLQTWVFGVADHGEF
                     LDRLGGDRLKQLVPQESLVAAPVNFGY"
     gene            5993..6529
                     /locus_tag="VF089_08585"
     CDS             5993..6529
                     /locus_tag="VF089_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX6174058.1"
                     /translation="MRTIQACVSRARDGGIAITYSLEGQISRLCLPARSAPMRVHGLW
                     RHTCFEAFLSVEAAPRYYEFNFAPSGQWAAYAFQSYREGGPLPQASDPQITVRTSIDR
                     FELDALICRESLSALPLNARLLLGLSAVIEENDGVVSYWALKHPPGPPDFHYASGFVL
                     ELEPHVAAALDPAYTGKR"
     gene            6526..7731
                     /locus_tag="VF089_08590"
     CDS             6526..7731
                     /locus_tag="VF089_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX6174059.1"
                     /translation="MKFGIDRLLKEPALRRALKGRRSALLAHPASVTRHLQHSLDALA
                     GCHDCQLTAAFGPQHGLRGDKQDNMIESPDFIDPIHGIPVFSLYGEVRRPTVAMMDTF
                     DVLLIDLQDLGCRIYTFVTTLRYMLEAATERKKSVWVLDRPNPIGRPVEGLRLRDGWE
                     SFVGAGPLPMRHGLTLGELAHWFVRMLRLDVDCEVLTMDNWQPSIEPGFGWPLGERSW
                     VNPSPNAPNLSMARCYAGTVMLEGTTLSEGRGTTRPLELLGAPDLKPRDLLAMMDSLA
                     PQWMRGCRLRDCWFEPTFHKHVGKLCGGIQIHVDDPAYDHNLFQPWRLLALALKSLRT
                     LRPDYPLWRDFSYEYETGRLAIDVINGSELLRQWVDDPNAAPADLDDLAKPDEASWLE
                     ERESLLLYR"
BASE COUNT         1696 a         2090 c         2176 g         1844 t
ORIGIN      
        1 gcaagaggct tttttcctag gaaatctagt tgcccggccg gtctcggttg gaagcatcct
       61 tattggaggt caacgtgaac agcaagccct ataagaacta tttcatcgac agtgagtcgt
      121 tccagcggga gaagcacggt agttgggttc cacaatacac gatagcgcgg cgagtaactg
      181 aatcgaatga tttcccttcg caccaatatc agttcagcca cacatttccg accaaaaatg
      241 aggccgattg tttcgcagtc gaaaaagcac aggaatggat cgacaagaac caaaagcgct
      301 gatcagccga tatcatcgag taaatacttc ggcgatgagg tcgcctcacc ggggtgcggt
      361 gccgtttgat cgtctgtcag agggctgaaa ctgatcggag cggcgagaca acagagcacc
      421 tgtcaggact gatgctctcg gattgtttga accgatattt ctctggggaa gatgaactca
      481 caacatcagc ttgaggcgcc gttcggcgcc tcaagctggt ttgctcgctg aaactctgct
      541 tggctgactt cgttcgttag tgtgaaattt tcaacgccat gcctagcggc aaagggctgt
      601 tgccgacgct cagagcggtg cctaatctct ctagctccca ggacgagacc ttttctactg
      661 ccataccttc tttaggagcg gtttccaatt tttcggcttg aggaagtttg accaggtatt
      721 tgacgttatt cccgtcgctc aacgtcaccg acaaaaccat ctgtttgcct tcttctgttc
      781 caagggtttg aatcgcggcg attcggctcg agaggctggt cttattgaca gtgtctgtat
      841 acagcccaat gaatgcgccg atgaaagcgg ccatcacaag ccagctatac tggcttaccc
      901 gtttctgccg tttcaaggat cggcttctct gaagatcgaa gaatttacgg taagattcct
      961 tttccgcggt caatttcttg agcggatctt gctccggcga gctcgccggt tgatcgtaag
     1021 aaagcccggt gctgttcaat atcatgccca ggatcaccag cgctaagggg atggcgatga
     1081 tcaatagagg attcataaaa ctcctttgag gcttttgata gcctttggat attggaagag
     1141 gaagggagtc agaaccgcta atatcctatc gatccggagc caaaggacat gccaccgatg
     1201 aggaggctgg ggccttttac ctcttacgcg ccagtacggc tgaaggaaat gtttacttgc
     1261 caactctatc cgaatggaag cactgtagca aggaggagcg ccgacagaag aatgtgagat
     1321 atgagtccgg gaaatagcgg cccgcctcgc tccgcttgat ttggatcaaa acaaactagt
     1381 tagcacaaac tgagatgttg tgcctgcgga tctcattggc atttcttcac tgagaatttt
     1441 tgcagtcttg aaccgacgcg gtaatttctc agccgagcca aaactctcgc aacttacgga
     1501 agtgttggcg gatcttcgca ccagaacaga ccaatggttt cgaacgcaat ctgccgggtt
     1561 cgagcttgcg catgtacgcg ccgccgtaaa tttgtccatc ccagcgcgcg tacatgcgat
     1621 gtgtgatctc atcggtggag gtcgtttgcc tgcgtggtga gaagtttcct gccgatgttt
     1681 aggtcgcttg gcgtatcttt ggtcctacgc ctgcggactc gatgttatag tttgaaacca
     1741 gcgttgttca acccttcttt gagttcggcc ttgtctttct cgctaaatgg catcgtttcc
     1801 caacgcggga agcgctccac ttgcaaaccg cggagcttca tcagctcgca gaacacgccg
     1861 cggccgtgag tcttcaaata cttgccaacc acctgcatga gccgggtgag cagatcgtgt
     1921 tccttttgtg cccctttgag gtcgcccagt ttcaaggctt cgtacatttt gacgcaggct
     1981 tccggaaaag gcgttgacgg cggatgaatc gcgccgcgca cgcccacgga gtaacccgta
     2041 tacagagaaa aaattccgcc tggaaaaact ccggtagatt tcggcagttg gtatacgtaa
     2101 cccagcagtt tgtcataggc gatgaaagaa actttgatac ccgcgagcgg gttgggcgcg
     2161 atcgcttcga tgatcttgtt agctttcgcc ggagtaatct cgacccatgt agtttcggtg
     2221 ttgtcgtaga taaagagcgg cacgcccggc accgacttgg ctatcgcctt gtaatgagcg
     2281 tacacctcgt aatcgtcatg gggataatag taggggggta caactccgat ggcatccacg
     2341 ccaaggctgg ccgcgtgctt tgccaattcg atggaggaat aggtattggc tgtgccgatg
     2401 tgcatgacca ccgggacttt accggcaact cgcttaacga tctgttcggc caccttcttg
     2461 cgctcgtcga tttccataac catgccttga ccgtgagtgc ctaaagggaa aaaaccgtgc
     2521 actcccgatg aaagatagaa atcgataatc gcatcagtcg cactgtgatc gatggcgcca
     2581 tcgttcttga acgccaccgg catcggcatc aaaatccctt cgatttttct gtcgctgggc
     2641 ataaagactc ctttcgggtt agatcacatt ttaagtttcc gtacccatat cgcaccgtcg
     2701 ccgacaattc cagacgatat ccgtttcgaa ctgaagccaa agatgataaa aatttatcat
     2761 cttacacagg aggcgttaat ggcagaaaag aatccgacgg taacggtatc gaccagcatg
     2821 ggagacatac gaatcgagct tgaatcggag aaggcgccga tcacgactaa gaacttcctt
     2881 gattatgtca gtgacggatt ttacgacggt ctgatttttc atcgggtgat tcccggtttt
     2941 atgattcagg gtggcggaat ggacccgcag atgaagcaga agagcaccaa gtcgccaatc
     3001 aagaatgagg cggcaaacgg gttgaaaaac aaaaacggca cggtggcgat ggcgcgaacc
     3061 aacgtcgtcg acagcgctac cgctcagttt ttcatcaacg ttaaagacaa cgacttcctg
     3121 gatcacagga gcacgtctgc ggatgcgttc ggatatgcag tcttcgctcg cgtcatcgac
     3181 ggcatggacg tggtgcagaa gattgaaaaa gtaaagacgc gtaacagcgg aatgcatcag
     3241 gacgttccgg tggatccggt tgtcatcaac tcggtgcgag tcgacggcta gaccggttca
     3301 ggcctatccc agcgggggca tgggatctcg cgatgcatcg gcggagtctc atgccccctc
     3361 taggcgacat acttcgtctg gcggtcgtca tgccgagttt gtgcgaccgc ttgaatcaac
     3421 actaccagtg aagccgaact tcagacgcgg acccggatag gacgcctagg acgtccagac
     3481 ccccttcgga tcgtattttc taatcgcaga cagttcatca tcggtcggag ccgcagtctc
     3541 cttgacgtcc ggcgatatct cgagcggcca acctgtttca ctttttatgt tctcgatgct
     3601 cactcccgga tgattggcgg tgagcatcat ttcatgggtg ttggaatcga atgagaatat
     3661 ccccatgctg gtaattaccc ggcgcggccc gccgccgatg agaccgttgc gtgaccgcca
     3721 atcgccgccg tcaccaaagc ctggagaggt gatgtaacga accctggctg gaaaacggcg
     3781 ccgttcgtga ttcatgatga tgatgcatcg cttagccatc gcggcgatgt ccgcagcgcc
     3841 accgctcccc ggcaaacgag tccgtttgcc gttctcttca acccagtgcg tgttcaggtt
     3901 gccaaaacgg tcgacttccg cgccgccgat aaaacctaag tcgatgcgat tactctgcaa
     3961 aagatgcatg acgtcgttga ggccggtgca actcaacgcg cgcgccacat tgggcggatc
     4021 gctcatcgtt agaatcgact cgagcgccgg ccagtctctg ataaccccgt tctcgaagat
     4081 accgagcgcg ctgggcgcat gagtctcctt ggctacggca tagccgagaa gcggcaggcg
     4141 catgccgaca aagactttat cgccgtcgcg tatttcccgg gaagcggcgg cgaccatcag
     4201 ttctcgtaac gtgtagttca tgtccagcag ggtggtaggt tggctattgc ttttaaattt
     4261 cagacaaaga ggggggatcc tggattccgg gttgcgctag ctacgccagc ttgcccgaaa
     4321 cgacgatcga attacgtgcg aatttcagtg acagcacatc aggctgcctt attgaacgca
     4381 tcaacgccgg cctggcaaat ctgtttgtcg acggcggcag gggcgccact gacaccgacg
     4441 gcggcaatgc actggccttc gatcgtgaga gggattccgc cttcgatgct gatgaaccaa
     4501 ccgggaccga cagccaaagt ctgcgccaaa acctggtgat cgggaatctc gccgccctcg
     4561 cggttggtct tcccggtcgc cgctgtggtc agcgcagccg accgggcctt ggcaaccgca
     4621 atcatcgtgt tgtgggtccg tccgttagtc attctcttga acgaaaacag gtgtccgcca
     4681 ctatcgacga ctgcgatact gacgatctgc ttgagctctt tggctttttc gatggccgca
     4741 ttgacaatga tgtcggcgcc ttcctgcgtt aacttgaaaa acggtactgc cttcataggt
     4801 cctcctacgt gatgatcctt tcctgtttaa tagccaaagt ttaccggcgc ggcgactagg
     4861 ctttcctgcg gcacgagttg tttcaaccgg tcgccgccga gccgatcgag gaattcacca
     4921 tgatcggcca caccgaagac ccacgtttgc agccatttct cgaatccatc ccgcgaacga
     4981 gtctccctgt ggtattcgaa atagaaagag tcatcccggc gcgtatagcc ttgagccggc
     5041 gaggggtgcg aaccaaatgg ctgatggacc acgctggcga ctaggaagcc gggaatcaat
     5101 gttcgatttg gatcgcttag gatcacatca tgatcgatga tttcttcgca ggtcagaata
     5161 acctttttag ccgcgaaagc agcttccttt atgacaccga agtttcccca ggcgtgcgcg
     5221 ttcccatcgc gatcggctct ttgcacatga agaattgcta cgtccggcgt gatcgctttg
     5281 acggctaaga gctcatcgcc ggtaaacggg catttcaccg agccgaactg cgggctatgg
     5341 ctgatgtcgc tgccgcgaat cgatttggtg ggcaaaaacg gcaacccaat ggctgcggcc
     5401 tgcaagccca ggccgatggt aaaattcgag tgctcttcaa tctcgatcgg gtgcggtatg
     5461 gcggattcgg ccgcccgccg gtagttgtga ccgaggccgg cggcgacatt gccgacccac
     5521 gaggcgatga tcttttttac gcaaccggcg ccgatcagtt ggtcgaattg catgtcggat
     5581 atcggcgcga tgagggtgag gtttttcttt cgctgacgta taatctcgta gctggcggcg
     5641 aatggaatca acgattcgag ggcgcacccc atggcgatgg aattgccgtc atcgatgtct
     5701 tgcgcaatag cgtcatggag acttcgaacc ttgtccatat tcgtcatctg tacccgctct
     5761 gtggatggag ttcaatgcaa ttggagcgcg cttgccacag ctgttctggg cttacgttgt
     5821 cagccaaacg tgtcggctat tgcggcaccg gcttggattc gcattcgctc gatcgcgaag
     5881 cagagacaga tccgttgatg gaggctttcg agtcgcgttc aaacctagca tgatgccttt
     5941 agtcgcacag gaacgggcct tggcttgtca tcccgagaag ccgagccgat cggtgagaac
     6001 gatccaagcg tgcgtgtcac gagctcgtga cggcgggatt gcgatcacgt actccctgga
     6061 gggacagatc agccggttat gtcttcccgc gcgatccgca ccgatgaggg tacacgggct
     6121 gtggcgacac acttgtttcg aggccttcct atccgttgaa gctgcgccga gatattatga
     6181 gtttaacttc gctccctccg gccagtgggc cgcctatgct tttcaaagtt atcgcgaggg
     6241 cggcccattg ccgcaggctt cggatccgca gattacggtt cgcacttcta tcgatcgttt
     6301 tgaactcgat gccctcatct gcagagaatc tttgtcggcg ctgccactga acgcccgctt
     6361 gttgttgggg ttgtcagcgg tgatcgaaga aaacgacggc gtggtgtctt actgggcact
     6421 caagcatccg cctggcccgc ccgactttca ctatgcgagc ggatttgttc tggaattgga
     6481 gccgcatgta gctgcggcgc tcgatccggc ttatactggc aagcgatgaa attcggcatc
     6541 gaccgcttgc taaaagagcc ggcgcttcga agagcgctga aaggtcgccg atcggcactg
     6601 ctcgcgcatc ccgcttcagt cacacgtcat cttcaacact ctctggacgc tctcgccgga
     6661 tgtcatgatt gccaactcac cgccgctttc ggtcctcaac acgggctgcg cggcgataag
     6721 caggacaaca tgatcgaatc gccagatttc attgatccga tccatggcat tcctgttttc
     6781 agtctctacg gtgaagttcg tcgtcctaca gtggcgatga tggacacttt tgatgttctg
     6841 ctgatcgatt tgcaggatct cggttgccgg atctacacgt ttgttacgac cttgcgttac
     6901 atgctcgagg cggcaaccga gcggaaaaag agcgtctggg ttcttgatcg tcccaatcct
     6961 attggacgcc cggtcgaagg gctgcgcttg cgcgatggat gggaaagttt cgtaggagcc
     7021 ggaccgttgc caatgcgtca cggcctgacc ttaggtgagc ttgcgcactg gttcgtgcgc
     7081 atgcttcggc tggacgtcga ctgcgaggtc ctgacgatgg ataattggca gccgtcgatt
     7141 gagcccgggt ttggttggcc tctcggtgaa cgaagttggg tgaatccgag cccgaatgcg
     7201 cccaatctct cgatggctcg ctgctatgcc ggaacggtca tgctcgaagg caccactctg
     7261 tccgaggggc gcggcacgac acgtccgttg gagctcttgg gcgcgccgga tctcaagccg
     7321 cgcgatctac tcgcaatgat ggactcgctt gcgcctcagt ggatgcgcgg atgcaggctg
     7381 cgtgattgct ggttcgagcc gacgtttcat aagcatgtcg ggaaattatg cggcggcatc
     7441 cagatccatg tcgacgatcc ggcatacgat cacaatttgt ttcaaccgtg gcggctcttg
     7501 gcgctggcgt tgaaaagttt gcgaacgctc cggccggatt atcctctgtg gcgcgatttc
     7561 tcctacgaat atgaaaccgg acgccttgcc atcgacgtta tcaacgggag tgaattgctg
     7621 cggcaatggg tggatgatcc caatgccgcg ccggccgatc tcgacgatct cgccaaaccg
     7681 gatgaggctt cgtggctaga agaaagagag tctctgttac tgtatcgatg actgatcaat
     7741 gtagcggcag taggcccccg acgctaaagc cccgtgcaga agttaacacc gaagggggcg
     7801 cacccg
//
LOCUS       DASUTG010000375         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_355741, whole genome shotgun sequence.
ACCESSION   DASUTG010000375 DASUTG010000000
VERSION     DASUTG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355741"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF089_08595"
     CDS             complement(<1..341)
                     /locus_tag="VF089_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174060.1"
                     /translation="MFTDKVDLEDFEKQDPEYQDLLKRVLAIQADCEIGGPNLYVKNI
                     LPSAPTKLDQLIVARTAAEEMDHYRKMARIAGDIGLDVSYVLSRPNQERYVEAFRGSI
                     DTWEDYAVFGF"
     gene            complement(880..1872)
                     /locus_tag="VF089_08600"
     CDS             complement(880..1872)
                     /locus_tag="VF089_08600"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6174061.1"
                     /translation="MSGGQRRSVILGTGSELPKKIITNHDLEKMVETSDEWITVRTGI
                     KERRILEEGKGNADMAFHAATRALEDASLDADDLDAIIMGTVTADYPFPSSACVLEDM
                     LGARGVFSFDVGAACSGFLNALSVADSFIRLGKIRNALVVGSDALSRLLNWQDRTTCI
                     LFGDGAGAVVLGASENGNGILSTKLRTDGSYAKTLYVPAGGSLKPANCKTVERNEHTI
                     TMNGKEVFKIAVRSMEDISREALEEAGVGIDEVSLVIPHQANRRIIVALADRLGVSMD
                     KVMVNLDKYGNTSAASIPVALDEAKRGGRIQKGDVVLLNAFGAGFAWGAAVIKF"
     gene            complement(1869..3122)
                     /gene="fabF"
                     /locus_tag="VF089_08605"
     CDS             complement(1869..3122)
                     /gene="fabF"
                     /locus_tag="VF089_08605"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6174062.1"
                     /translation="MNTAEQRRVVVTGMGLVTPLGTGVEKNWEALIAGRSGIRRVSRF
                     SNLEAYPSQIAGEVPDFSPTDFIELKEIKKMDLFIQYAVAAAAMAMTDSGLKIDADLA
                     EQVGVIIGVGLCGIDTIETTKEAILQGGPRKISPFFIPKVISNLAPGQIAIRHGAKGI
                     NLTPTSACASGTHAIGEAYHLIRRGLQDAVITGGAESAITPLAVGGFAAMKALSTRND
                     EPERASRPFDRDRDGFVIAEGSGVLILEERERALHRGAKIYAELVGYAANGDAHHMTA
                     PSPEGEGAARCMKLALKDAGLAPTDVDYINAHGTSTEYNDANETMAIKRVFGEQAAKL
                     AVSSTKSMTGHLLGAAGAVEGVFSALTLHHGLIPPTINYDNPDPQCDLDYVPNEARKA
                     DLKVVLSNSFGFGGTNACVIMRRAE"
     gene            complement(3199..>4109)
                     /locus_tag="VF089_08610"
     CDS             complement(3199..>4109)
                     /locus_tag="VF089_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX6174063.1"
                     /translation="PARCQLLPPRAASRSEITLCHDPEYVRLVESTASLSQYALDGDT
                     ITSPESFAVASLATGGFLRLLDSVAAGESANGFAIVRPPGHHALRNRAMGFCLFNTIA
                     VGAQYLKTAHGAKRIAIVDWDVHHGNGTQEAFYDDPCVLFVSAHQYPFYPGTGGIAEV
                     GVKEGTGFTINIPLPAGCGDAEYLRVFRDVVIPAIERFGPQWILVSAGFDPHRQDPLA
                     GMTVTEAGFSQMARAMLQLAEVYCGGKIAFLLEGGYDLTALRSSVAGVLETLQQSGGD
                     KFPSDVGGDKIDLLIRAVLRTHDQFH"
BASE COUNT         1003 a         1151 c         1006 g          949 t
ORIGIN      
        1 aaaaatccga acacagcata atcttcccag gtatcgatcg agccgcgaaa tgcttcaacg
       61 tatcgctcct ggttggggcg gctcagcacg tatgatacat cgaggccgat atccccggcg
      121 atccgagcca tttttctgta atgatccatc tcctcagcag ccgtgcgcgc tacgataagc
      181 tgatccagtt tagtcggcgc ggagggtaaa atattcttaa cataaagatt cgggccgcca
      241 atttcacagt cagcctgaat cgcgagaacc cgcttcagca agtcctggta ctccggatct
      301 tgtttctcaa aatcttcgag atcaacttta tctgtaaaca tgttatgggg gcctcctgtg
      361 gctcgctaat tatgcgcaat tattatggaa atttttagca aaacagttct gataaatcaa
      421 taagcggcgc cgctccagaa gcaaatccgg ctgttctagg agaacactta tgatggccca
      481 tgaatcaccg gcacttttga cggatctctg caaattgaca aacgtttatc atcaaccaaa
      541 tcaacctgca gtcgttgatt gccacagaag cggctccgtg tgttcatgcc gcaggcaacc
      601 ggccagtgtg gattttgcct tacgccgaac gcatggaatc gatgcccagt cgagttcagt
      661 gcagcactcg cgattgcgtg aagagacgga gcgacagcta atcaagcttt acgcgcgcag
      721 acccttctgc tagcctcgaa gaaggtcaga actcgttcag gggaaaatca gcttcaacta
      781 aataaggggg aggtgtaaaa tagcatatat aatactgggt gtagatgaaa cggatgcggt
      841 taatcactgg tggaaatcgt tagaagaggt ttaaacgaat caaaacttta tcaccgctgc
      901 gccccaggca aatcccgcac caaatgcatt gagcaaaacg acgtcgccct tctgaatacg
      961 acccccacgt ttggcttcat ccaaagccac gggaatggac gctgcagagg tattcccgta
     1021 tttgtcgaga ttgaccatca ctttatccat cgaaacgccc agtctgtcgg ctaaagctac
     1081 gatgatgcgc cgattggcct gatgtggaat caccagtgac acttcgtcga ttccgacacc
     1141 ggcttcttcc agtgcctcac ggctgatatc ctccatcgag cgcaccgcga ttttaaaaac
     1201 ctctttccca ttcatggtta tagtgtgctc gtttcgttcc accgtcttgc aattggctgg
     1261 ttttaaagag ccgccggctg gaacgtatag agtcttcgca taagagccat cggtgcgcag
     1321 tttggtgctt aagataccgt taccattctc tgatgcgccg agcaccacgg cacccgcccc
     1381 atcgccgaac agaatacagg ttgtgcgatc ctgccaattc agcagccggc tgagagcatc
     1441 agaaccgacg acgagagcgt tgcggatttt gccgagccgg ataaacgaat ccgctactga
     1501 taaagcattg agaaatcccg agcaggcggc gccgacatcg aacgagaaaa ctcctctcgc
     1561 tccaagcatg tcttcaagca cgcaagccga acttggaaag ggataatccg cagtcaccgt
     1621 tcccatgatg atcgcgtcca aatcatcggc atccaagctc gcgtcttcga gcgctctcgt
     1681 tgcagcatgg aacgccatat cggcgttacc cttaccctct tcaagtatgc ggcgttcttt
     1741 tatcccagtg cgtacggtga tccactcgtc actggtctcg accatcttct caagatcgtg
     1801 gtttgtgatg atcttttttg gtagttcaga gccggttcca aggatcacac tgcgacgctg
     1861 ccctccactc attccgccct cctcattatc acacatgcat tcgtcccgcc gaaaccgaag
     1921 gaattcgaga gtacaacctt tagatccgcc ttacgggctt cgttgggcac ataatccagg
     1981 tcgcattgcg gatcaggatt atcatagtta atcgtcggcg gaatcagacc atgatgcagg
     2041 gtaagcgccg agaagacccc ttcaacggcg cccgccgcgc cgagtaaatg gccggtcata
     2101 gatttagtgg aactcactgc tagtttggcc gcttgttcgc cgaacacccg tttgatcgcc
     2161 atcgtttcat tggcatcgtt atattctgtg gaggttccat gtgcgttaat gtaatcgacg
     2221 tcagtgggcg cgagccccgc atctttcaat gccagtttca tacagcgggc ggcgccttcg
     2281 ccctcgggcg acggcgcagt catatggtgg gcatcgccgt ttgctgcata cccaaccaat
     2341 tctgcgtaga ttttcgcgcc tcggtgaaga gctctctctc tttcttcgag aatgagaaca
     2401 ccggagcctt ccgcgatcac gaacccatcc cgatctcggt cgaagggacg gctcgcacgt
     2461 tcaggctcat cattgcgggt cgacagcgcc ttcatagccg caaagccgcc aacggcgagc
     2521 ggggtaatag ccgactcagc acccccggta atcactgcat cctgtaagcc ccgccggatc
     2581 aagtgataag cctcgccgat ggcatgtgta cccgaagcgc atgcagatgt cggtgttaaa
     2641 ttaatccctt tagcaccatg gcgaatcgcg atctgaccag gagccaagtt agatatcact
     2701 ttcggaatga aaaaaggaga tattttccgc gggccgccct gcagtatagc ctccttggta
     2761 gtctcgatgg tatcgattcc gcaaagtcca acaccaatga tgacacccac ctgctcagcc
     2821 aggtcggcat caatcttcaa gccgctatcc gtcatcgcca tcgctgccgc cgccaccgca
     2881 tactggataa aaaggtccat cttcttgatc tctttgagct cgatgaagtc tgtaggagaa
     2941 aagtccggca cctccccagc gatttgcgac ggataagctt caagattgga aaatcggctg
     3001 actcttctaa tcccggatcg cccggcgatc agagcctccc aattcttttc aacgccagtt
     3061 cccaatgggg tgaccaatcc catgcctgtt acgacgaccc tgcgttgttc agctgtattc
     3121 acgttctacc aaccttttaa aatttaaacc ttaactttaa tactaaaata gatgactcgg
     3181 agctgagggt caatacattc agtgaaactg atcatgcgtc cgcaagactg cacgaatgag
     3241 aaggtctatt ttgtcgccac cgacatcgga cggaaacttg tcaccgccgg attgttgaag
     3301 cgtttcgaga acgccggcga cagacgatct cagcgcggtg agatcatacc cgccttcgag
     3361 taagaacgcg attttgcccc cgcagtaaac ttccgctagc tgcagcatcg cgcgcgccat
     3421 ctgagagaat ccggcttccg taacagtcat tcccgccaac ggatcctggc gatgcggatc
     3481 aaatccggct gatactaaga tccactgcgg cccgaaacgt tcaatagccg gaatgacgac
     3541 gtctctgaag actcgcaagt attccgcgtc gccgcagccg gctggaagcg gaatgttgat
     3601 cgtgaaaccg gtgccctctt tgactcccac ttctgcgatt ccaccggtgc ccgggtagaa
     3661 aggatattga tgggctgaga caaacagtac gcaggggtca tcgtaaaacg cctcttgggt
     3721 accattaccg tggtgcacgt cccaatcaac gatggcgatc cgctttgcgc catgggcggt
     3781 ttttagatac tgcgcgccga cagcaatggt attaaaaagg cagaacccca tggctctgtt
     3841 tcttagcgca tgatgtcctg gtgggcgtac tatggcaaaa ccgttggccg actcaccggc
     3901 agcaacggag tccaacaatc tcaaaaagcc tccagtagca agcgaagcga ccgcaaaaga
     3961 ttccggactc gtaatagtgt ctccatccaa ggcatactgg ctcaggctcg cggtcgattc
     4021 tactaaacgg acgtattccg gatcatgaca aagcgtaatc tccgatctgg aagcagctct
     4081 gggaggcaac agctgacaac gagctggat
//
LOCUS       DASUTG010000376         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_800422, whole genome shotgun sequence.
ACCESSION   DASUTG010000376 DASUTG010000000
VERSION     DASUTG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800422"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF089_08615"
     CDS             complement(<1..437)
                     /locus_tag="VF089_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174064.1"
                     /translation="MDNVALITGATRGIGRAIAAAYLREGARVFICARNQQEVAAAVR
                     ELRDDTTDAQVDGCAGDVGKETDVCRIVDAAIERFQTVDTLVNNASLLGPRVALVHYP
                     FEQWEEVIRVNLHGVFLLSQQVVKRMMAQRHGSIINVSSGVGRV"
     gene            507..1433
                     /locus_tag="VF089_08620"
     CDS             507..1433
                     /locus_tag="VF089_08620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX6174065.1"
                     /translation="MSTHQRLLVFLVCVLALTSILSPWFAIAADWVSGQLPGVERFPF
                     SRIFNRTFMFSGIVLFFLCRRLLRIGNLAQLGFVSLRQGSGDLLLGWSLAVGSLAALL
                     LAMSLAQVFEPYFRLSLARSLSRLFSALLSGVFAGSLEEIFFRGILFKGLLQSGKLFR
                     AFVLSSLFYSALHFVKPGEAYFLDGVEPLAGFRHLFFTFTPFSEPLDILPGMFSLFLL
                     GIVLSYAFFRTGNLYLSIGLHAGWIFGLKSVRVFGDYTRGDLGWLFGSADPKIVSGVA
                     AWIGIISVFLLVHWLTRNRCGLANDRTARSEL"
     gene            1524..2306
                     /locus_tag="VF089_08625"
     CDS             1524..2306
                     /locus_tag="VF089_08625"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6174066.1"
                     /translation="MGEQQPKIVTGIGKAELHRILVRGYDLNKELVGKITFTDMTSLM
                     LRGRLPTPNEAKMLDALLVILVEHGMVSHVIAARLVYHCAPEAIQAAVAAALCGAGSV
                     HLGSSEWSAKMLTEALPSETKNPDLEEIATAILDDYSARKQRIPGIGHRTHAEGDPRA
                     ETLFGIAKENGIYGKYCELLQKLSEVASQRRKRLIPVNVTGAIAAIALDMGFPWQITK
                     AFALIGRTLGVMGHIAEEIRNPMATQIDAAIKQAIVYEPYRT"
     gene            2490..3254
                     /locus_tag="VF089_08630"
     CDS             2490..3254
                     /locus_tag="VF089_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6174067.1"
                     /translation="MTYQNILTGQIDKVALITLNRPEKLNAMSYELACELDAELAKLE
                     SDDTVNAVVLTGAGPRAFSAGGDIHQMAKSTDEELAARTEFRTEANWRIATFTKPIIG
                     AINGLAYGAGAQLTSMLDIRIGCEITEFRFLAARYGRVNSSWSLPLVVGIAKAKELLY
                     TGRVVNAEEAEKIGLLNQVVASGELLAKTIEMAQLISENDPRMVQGIKRLLHENIGRD
                     WRGRYDNEQNARKSRLKSNHPREGFKEFLSRKGIRN"
     gene            complement(3339..>4218)
                     /locus_tag="VF089_08635"
     CDS             complement(3339..>4218)
                     /locus_tag="VF089_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6174068.1"
                     /translation="IDEFKHLQKNGYGAVYFVDDHFLLQPKRIEAICRGINDAGVNIQ
                     WGCEGRVDSTAQHLFPAMAKANCRTLMFGIESGSQKVLDRLKKDQTLEQVESAVDNAK
                     EAGIEIVHGFFVVGSPDETVEDMRATFDFASRLKLDTFGFNRLCVYRGTPLWKEYVAR
                     DLVDDATDWYKYFKCSEIDPTCLSGAIINDERTAGLRRLFLYKLTHYPLQTFRLLRRF
                     LRHMPLRDVIYLITKPFMGKNQGPTKNEVLSRAVEHRTLKDNAADLTNVPDALLERAI
                     EGEKSADNRPKRVALG"
BASE COUNT          909 a         1145 c         1142 g         1022 t
ORIGIN      
        1 acacgcccaa cgccggatga gacattgata atggaaccat gacgttgcgc catcatacgt
       61 ttgaccacct gctgggatag aagaaacact ccgtgtaagt tgacccgaat cacctcttcc
      121 cattgctcaa atggataatg gacgagagcc acccgcggcc cgagcaagct tgcattgtta
      181 acgagcgtgt caacggtttg aaatctttcg atcgcggcat caacgattcg gcaaacatcg
      241 gtctctttgc cgacatcacc tgcgcaacca tcaacctgcg cgtccgtggt atcatcacgc
      301 aattcgcgca ccgcggccgc aacctcctgc tggtttcgcg cgcagataaa cacgcgggcg
      361 ccctcacgca ggtacgcagc ggcgatggct ctgccaatgc cgcgagtggc tccggtgatc
      421 agagcaacgt tatccatcag ttttttccga tccgtgcttg tcatacttca gatcgtatac
      481 catagttaac tttttccatg aaatccatga gcacgcatca gagactgctt gtttttcttg
      541 tttgtgtgtt ggcgctgacg agtattctca gcccttggtt tgccatcgcg gcggattggg
      601 tgtccggtca attgccggga gtggaacgct tccctttctc acgcattttc aaccgcacat
      661 tcatgttttc ggggatcgtg ctatttttcc tctgccgccg cctccttaga attggcaatc
      721 ttgcccagct gggttttgta agtctacgac aaggcagcgg cgatctgctt cttggctgga
      781 gcttagccgt gggatccctt gcggcgttac tgttagcgat gtcactcgct caggtctttg
      841 agccttattt caggttgtca ttagcgcgat cgctatcgcg tttgtttagc gcgctgctgt
      901 cgggagtttt tgccggatca ttagaagaaa tatttttccg cggaatctta ttcaagggac
      961 tgcttcagag cggcaagctt tttcgagcgt ttgtgctgtc aagcctattc tattccgctt
     1021 tgcatttcgt caagccggga gaagcctact tcctcgatgg cgttgagcca ctggcgggat
     1081 ttcgtcatct atttttcacc ttcacgccgt tttcagaacc gttggatatt cttcctggaa
     1141 tgttcagtct gtttctgctc ggaatagtgt tgagctacgc atttttcagg acaggaaatt
     1201 tatatctatc aattggcttg catgcgggat ggatttttgg cttgaagtcg gtgcgggttt
     1261 ttggtgacta cacgcgcggt gatctaggct ggcttttcgg ttctgcggat cccaagatcg
     1321 tcagcggagt cgccgcctgg atagggatca ttagcgtatt cctgctggta cactggttga
     1381 cgcgaaatcg ctgcggactg gcaaacgacc ggacagcgcg ttcggaactt tagggcctcc
     1441 aactcgcttg attccctggt tagccgttgc gacctgcgat cgcgtgtcct agaatccgcc
     1501 catctaaatc aatgggagga tccatgggag aacaacaacc gaaaattgtg accggtatcg
     1561 gcaaggccga gcttcaccgt attctcgttc gcggatacga tcttaataaa gagctggtag
     1621 gtaagatcac tttcaccgat atgacatcgc tcatgctgcg cggccggctg cccacgccta
     1681 atgaagcgaa aatgctcgat gcattgttgg tgattcttgt cgagcatggg atggtttcgc
     1741 acgtcattgc cgcccggctt gtttatcatt gcgctcccga ggcaattcag gctgcggtcg
     1801 ccgcggcgct ttgcggcgcc ggtagcgtgc accttggatc gtctgaatgg tcggccaaaa
     1861 tgcttaccga ggcgctgccc agcgaaacca agaatcctga cctcgaagag atcgcgactg
     1921 cgatcttgga tgactattcc gcgcggaaac agcgcatccc gggaatcggc caccgcactc
     1981 atgccgaggg cgatcctcgc gccgagaccc tcttcggaat cgcgaaggaa aatggaatct
     2041 acggcaagta ttgcgagctg ctgcaaaagc tttccgaagt tgccagtcaa cgaaggaaga
     2101 ggttgattcc agtcaacgtc accggtgcaa ttgcggcgat tgctctggac atgggctttc
     2161 cttggcaaat caccaaagcc ttcgcgctga tcggccgcac gcttggcgtc atgggtcaca
     2221 ttgccgagga gatccgtaac ccaatggcga cgcaaatcga tgcagcgata aaacaggcga
     2281 tagtctatga accttatcgg acctgagaat gcgcctgaac tacaggcggg tgattttcgt
     2341 agcttgatcc tgctgggtag acgagtgttg ctcatgcact cggaatccgc cggtaaggtc
     2401 agccgtcgtc gtatgccgcc gaagtttccg ctaccgcatt tctgctgtcc cggcgccgat
     2461 catgatccac tgtgcttgaa ggagatcaga tgacttacca gaacattttg accgggcaga
     2521 ttgacaaagt tgctttgatc actctcaatc gtccggaaaa gctaaatgcg atgagctacg
     2581 aattggcttg cgagctcgat gcggagctgg cgaagctcga gagcgacgac acagtcaacg
     2641 ccgtggtttt gaccggcgcc gggccacgcg cattctccgc gggcggcgat attcatcaga
     2701 tggccaaatc aaccgatgag gaacttgccg cccggaccga atttcgcacc gaggccaatt
     2761 ggcgcatcgc tactttcacg aagccgatca tcggtgcgat caatggtttg gcttacggcg
     2821 ccggagcgca actcacttcc atgctcgaca tccgcatcgg ttgcgagatc accgagtttc
     2881 gtttcctggc agcgcgctat ggacgggtca attcatcttg gtcgctgccg ctggtggtag
     2941 gtatcgcgaa ggccaaagag ctgctctata caggacgagt ggtcaatgcc gaagaagctg
     3001 agaagatcgg gctgcttaat caagtcgtgg cctccggcga gctactcgcc aagactattg
     3061 aaatggctca actcatcagt gagaacgatc cgcgaatggt gcaggggatc aaacggctac
     3121 tgcatgagaa tattggccgg gattggcgcg gccgctacga caatgagcaa aatgccagaa
     3181 agagccggct caaatctaat cacccgcgcg aaggcttcaa ggagtttctt agtcgcaagg
     3241 gcatccgcaa ttagcaacac ggctttttgg aacacctggc gtcggtaagc ctgccgagcg
     3301 gcggctggtt agatgctggc tcttagcccg ctgtggcctc accctagcgc gactcgctta
     3361 ggccggttat ccgccgactt ctcgccttca atggcccgtt cgagcagcgc gtcggggacg
     3421 ttagtgagat ccgcggcatt gtctttgagc gtgcggtgct cgaccgcgcg tgacaacacc
     3481 tcgttcttgg tcggaccctg gttcttaccc atgaacggct tggtgattag gtaaataacg
     3541 tcgcgcagcg gcatatgtcg caaaaagcgg cgcaacagac gaaaagtttg cagcgggtag
     3601 tgcgtcagct tatatagaaa cagccgccgc aggccggcgg tgcgttcgtc attgattatc
     3661 gctcccgaca ggcacgtggg atcgatctcg gaacatttga aatatttgta ccagtcggtc
     3721 gcatcatcaa ctaaatcgcg cgcgacgtac tccttccaaa gcggcgtgcc acgatagaca
     3781 cacaaacgat tgaagccaaa ggtatcgagc ttcaatcgcg aagcaaagtc gaaggtcgcg
     3841 cgcatatctt caaccgtctc gtcgggactg ccgacgacaa agaaaccatg cacgatctcg
     3901 atgcctgctt ctttggcatt gtcgaccgcg ctctctacct gttccagagt ctgatctttc
     3961 tttagccgat ccaaaacttt ctggctgccg ctttcaatgc cgaacatcaa cgtacggcaa
     4021 ttggccttgg ccattgccgg gaacaggtgt tgggcggtcg aatcgacacg gccttcgcag
     4081 ccccattgaa tgtttacccc cgcatcattg atgcctcggc agatcgcctc aatccgctta
     4141 ggctgcaaca aaaagtggtc gtcgacgaaa taaacggcgc catacccatt tttttgcaaa
     4201 tgcttgaact catcgatc
//
LOCUS       DASUTG010000377         6481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_800517, whole genome shotgun sequence.
ACCESSION   DASUTG010000377 DASUTG010000000
VERSION     DASUTG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800517"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF089_08640"
     CDS             complement(<1..403)
                     /locus_tag="VF089_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6174069.1"
                     /translation="MFYLLKLSVFMIITVPAAVLTIFLGLFDRHGKAAYRASQIWTAL
                     ILRIGGVVLRVSGREHIDSRQQYVFMVNHQSNLDIPVLVQSLVSFQLRWIAKKELLWI
                     PFFGWAMWASRHITIDRADPLTAIKSLNRAKE"
     gene            592..1017
                     /locus_tag="VF089_08645"
     CDS             592..1017
                     /locus_tag="VF089_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6174070.1"
                     /translation="MSVNKVILIGNLGKDPELRFTSTGSAVARFPIATSEVWNDRDGN
                     KQERTEWHNIIVWGKQGESCSQYLAKGRQVFVEGSIRSRSYDDKSGTKRYVTEIVAQR
                     IRFLGGGGGTRMAPEIEGGASDEIPSGGAQAPFDDDIPF"
     gene            complement(1028..1999)
                     /locus_tag="VF089_08650"
     CDS             complement(1028..1999)
                     /locus_tag="VF089_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6174071.1"
                     /translation="MTLPKIVFLDAATFGDVSLEPFGEIGDCKIYQVTKPNETIGRLA
                     GQEIVVTNKAAIDATALAAPEAHNLLLIAVAATGTDIIGLPAARRRGIKVCNVPGYAA
                     QSVAQFTMALILELATHAGRYAADVKAGMWQKSPVFSLLTYPSTELNGRTLAIIGYGN
                     IGREVARMARAFGMEVMLAARPGAAEPPTSDRVPLEQLLPRADIVSLHCALTPQTRNL
                     INEERLKLMKPSAFLINTARGGLIDEAALIQALSERRLAAAALDVISEEPPAADHPII
                     VAARALDNLLVTPHTAWSARQARKRLLSEVAENISAFLAGRDRNLVG"
     gene            complement(1996..2811)
                     /gene="mazG"
                     /locus_tag="VF089_08655"
     CDS             complement(1996..2811)
                     /gene="mazG"
                     /locus_tag="VF089_08655"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX6174072.1"
                     /translation="MSDHKKTAEKFSQVLELMAQMRGESGCPWVREQTHQSLKPFLLE
                     ESHELLDAFTGEDPATIVEELSDLLYQIVFHCQIGAENGTFTAAQVISNLAAKIVRRH
                     PHVFGGDALPDSAAVTKQWGQIKAQERLADTAGSALGSLPKSMPALARAQAISERAAR
                     VGFDWPTIEPVWQKVEEELSELKHAWASGDRPRAGEELGDVFFSLVNLARFLGVQAED
                     VASQTTDRFIERFTYVDNQLRAAGKTPEQSSLEEMDRLWDEAKALERRGRLRP"
     gene            2951..3799
                     /locus_tag="VF089_08660"
     CDS             2951..3799
                     /locus_tag="VF089_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6174073.1"
                     /translation="MSTATIDGLQVHYQVFGSGAPLLLFAPGGFDATIEKWRAASAWT
                     GIDALEALATRFQVIAYDRRESGKSGGRVERLSWHTYARQAKVLLDQLKIDSALLLGG
                     CMGCSVALAFAVDYPEATRALLLHWPVGGFRWKASGLDRFQRHYRFASTNGLAGVNER
                     ARIGKSFWTDPEAGPWASVIAREPTFAEDFIRQDLNRYLGLIAVSGRGLFDRDTATGA
                     EPEEIMGISAPAFIVPGDDASHATSAAHYLRELLPNASFWNVPPTEQTTREVCDRILE
                     FSRQLS"
     gene            3914..4633
                     /locus_tag="VF089_08665"
     CDS             3914..4633
                     /locus_tag="VF089_08665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6174074.1"
                     /translation="MDVTTSTLQLSTADGNMDAFEARPKDGGSYPGIVVLMEAFGLND
                     HIKKVTERIAQEGYVAIAPDLYHRESERIIPYSDMKKAIGTMNRLQDPKVMEDVGAAI
                     AHLKSQSNAKSGAIGVTGFCMGGRYTYLAAAHHNQDVKAAVCFYGGGIPMGDPSPLSR
                     TGEITCPIYLFFGAKDPLIPMDQVAQINTELTHKKVSFSMKVYPEATHGFFCDERSSY
                     HESAAKDSWEKMKTFFAQHLK"
     gene            4719..5615
                     /locus_tag="VF089_08670"
     CDS             4719..5615
                     /locus_tag="VF089_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX6174075.1"
                     /translation="MPGNKNKDIVTLGLVQMSASDDTEANLAKAEARIREAAKRGAQI
                     VCLQELFRSRYFCQSEDQRCFNLAEAIPGPSTDRLSALARELQVVIVASLFEKRSAGI
                     YHNTAVVLDADGSIAGKYRKMHIPDDPLYFEKFYFTPGDLGFPSFQTRYARVGALVCW
                     DQWFPEAARLASLSGAEILVYPTAIGWIPKEPRAVAQKQRDAWELIQRAHAIANGVFV
                     ASVNRVGREGSLKFWGHSFVAGPSGEILNVAAADREEILIAKCDLRQIDEIRESWPFF
                     RDRRIDAYSSLQSRFIDSPDGT"
     gene            5654..>6481
                     /locus_tag="VF089_08675"
     CDS             5654..>6481
                     /locus_tag="VF089_08675"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX6174076.1"
                     /translation="MPRDLGYSMPAEWVPHRATWLSWPHNLETWPAQLERVREAWVQM
                     ILALSPHEKVCLLINDDLSRQDVTLRLKTAGAVMENVALIKIPTVDVWMRDYGPTFLV
                     RNQVDGPLAINDWIFNGWGGKYKAYEHDDQVAKSIAELLGMPVFAHGLVLEGGAIEVN
                     GAGICLTTEQCLLNTNRNPDLSRGEIEQFLRDSLGVSQIIWLGEGVAGDDTDGHIDDI
                     ARFVNARTIVCAAEDDPKDANYAPLQENLERLEGARDALGSRFDLLTLPSPRPVICQG
                     "
BASE COUNT         1470 a         1735 c         1807 g         1469 t
ORIGIN      
        1 gttctttagc gcggtttaag ctcttaatag cggtgagggg atccgctcga tcgattgtaa
       61 tatgcctgga ggcccacatt gcccagccga aaaacggaat ccaaagaagc tcttttttgg
      121 cgatccagcg caactgaaaa ctcaccaagc tttgtaccag tactggaatg tcgaggttgc
      181 tttggtgatt gaccatgaaa acatattgtt gtcgcgaatc gatatgttcg cggccgctaa
      241 ctcgcaatac aaccccgccg atacgtaaga tcaaagcagt ccaaatctgg ctcgcgcggt
      301 aggcggcttt cccatgacga tcgaaaagac caaggaagat cgtcagcacc gccgccggta
      361 cggttatgat cataaaaacc gacagcttga gcaggtaaaa catcaagatc aaagtaagct
      421 tggcgagtgc aaaaccgcga aaaagcgtta gctaagtcta gtgtacgcat gaacaaaatg
      481 caattgagcg cgtgcccccc gatagctgcc aattctattg ttgactataa ataacctggg
      541 cgctaagttg gctcgactgt ttgctgccgc gacagcggct ggaggagttc aatgtctgtt
      601 aacaaagtca tactcatcgg taatcttggg aaagatcctg agcttcgttt cacttcgacc
      661 ggtagcgcgg tagcgcgttt tccgatcgct acttccgaag tttggaatga ccgcgatggc
      721 aacaagcagg aaagaaccga atggcataac atcatcgtgt ggggtaagca aggtgaatct
      781 tgcagccaat acctcgccaa gggcaggcaa gtttttgtcg agggcagtat ccgcagtcga
      841 agctacgacg ataagagcgg taccaaacga tatgtgaccg agatcgtcgc acaacgcatt
      901 aggtttctcg gcggcggcgg tggcacgcga atggcgccgg aaatcgaagg aggtgccagc
      961 gacgaaatac ccagtggggg tgcacaggcg ccttttgatg acgacatccc tttttgaagc
     1021 atcttctcta tccgaccaga ttcctatctc taccagctaa aaaggcggag atgttctcgg
     1081 ccacttcgct cagcagcctc tttcgtgctt ggcgcgcgct ccacgcggtg tgcggcgtta
     1141 ccaacagatt atccagtgca cgggcggcga cgataatcgg atgatcggcg gccggaggtt
     1201 cctcgctgat gacatccaac gcggccgcgg ctagtcggcg ctcgctcagt gcctgaatca
     1261 gtgctgcttc atcgattaat ccgccgcgcg cggtattgat caagaatgcc gatggtttca
     1321 tcaacttaag cctctcttcg ttgatcaggt ttcttgtctg cggcgtcagc gcgcagtgca
     1381 gcgatacgat atcagctcgc ggaagaagtt gctctaatgg aactcgatcc gacgtaggtg
     1441 gttccgctgc tcctggacga gcggccagca tcacttccat gccgaatgcc ctggccatcc
     1501 gggcaacctc gcggccgatg ttgccgtaac caatgatagc taacgtcctg ccgttgagct
     1561 cggtactcgg atacgtcagc agtgagaaca cgggactttt ctgccacatc ccagccttga
     1621 catcagcggc gtaccggccc gcgtgagtag ctagttctag aatcaacgcc atcgtgaact
     1681 gcgcgaccga ttgtgcggca tagccgggga cgttgcagac cttgattcca cgtctccgag
     1741 ctgccggcag gccgataatg tcggtaccgg tagcggccac tgcaatcaac aataaattgt
     1801 gcgcctccgg ggcggcgaga gccgtcgcat cgatggctgc tttgttggtg acgactatct
     1861 cctgaccggc caatcgacca atggtctcat tgggcttggt gacctgatag attttgcaat
     1921 cgccaatctc gccgaagggt tccagtgaaa catcgccgaa ggtggccgca tcgaggaaaa
     1981 caattttagg caaagtcatg gtcgaagtcg cccgcgccgc tcgagtgctt ttgcctcatc
     2041 ccacaagcga tccatttctt ccaggctgga ttgttcgggg gttttccccg ctgcccgcag
     2101 ctgattgtcg acgtaagtga accgttcaat gaacctgtcg gtggtttgac tagcgacatc
     2161 ctctgcctgg acacccagaa agcgcgcaag attcaccaat gagaaaaaca catcgcccaa
     2221 ttcctctccg gctcgtggac gatcgccgga agcccaggcg tgcttcagtt cgctcaattc
     2281 ctcctccact ttttgccaga ccggttctat ggtaggccaa tcgaacccca ctcgcgcggc
     2341 gcgctccgaa atcgcttgag cacgtgccaa cgccggcatc gatttcggca ggctccccaa
     2401 tgctgacccg gcagtgtccg caagtctttc ctgcgctttg atttgtcccc actgctttgt
     2461 cacggccgcg gaatccggca aggcgtcacc gccgaaaacg tgaggatgac gccggacaat
     2521 tttcgccgct aagttgctaa tcacttgagc ggcagtgaat gtgccgttct cggcgccgat
     2581 ctggcaatga aagacgattt gatacaacag atctgataat tcctcgacga tcgtagccgg
     2641 atcttcgccg gtaaacgcat cgagaagctc atgactctct tccaacaaaa acggcttcag
     2701 gctctggtgc gtctgctcgc gaacccatgg gcagccgctc tctccccgca tttgcgccat
     2761 caactccaag acttgggaaa acttttctgc agtttttttg tggtccgaca taatttaccc
     2821 gagaagcaca gcggcacgaa ttttcttgcg tgaagtagcg tgttagagct tacataatcc
     2881 aagcaaccct acccggtggc aatgcccggg ttcagtaaca aaatagagcc ttaatcgaag
     2941 gagtgaatca atgtcaactg caaccatcga tggactgcag gttcattatc aagttttcgg
     3001 gtccggtgct ccgcttttat tgtttgcacc tggcggattt gatgcgacca tagaaaagtg
     3061 gcgcgccgcc agcgcctgga ccggcatcga tgccctagaa gcgctggcga ctcggtttca
     3121 agtgattgcc tacgatcggc gcgaatcggg aaaatcggga ggaagagtgg agcgcctaag
     3181 ctggcacact tacgcgcggc aggcaaaagt tctactggat cagctcaaga tcgattctgc
     3241 gctactcctg ggggggtgca tggggtgttc ggtggcacta gcattcgcag ttgattatcc
     3301 ggaggcgacc cgggcgctgt tgctgcactg gccggtcgga ggttttcgtt ggaaagcgag
     3361 cggtctcgac cgatttcaac gccattaccg cttcgccagc accaacggtc tggcgggggt
     3421 taacgagcgc gcgagaatcg gcaaaagctt ctggacggat cccgaagccg gtccctgggc
     3481 ttcggtgatt gctcgcgagc caacatttgc tgaggacttt atccgccagg atttgaatcg
     3541 gtatctcggg ctcattgcag tcagcggcag gggcttgttc gatcgcgaca ctgccacagg
     3601 ggcagaaccg gaggagatca tgggcattag cgcgccggcg ttcatcgttc ccggtgacga
     3661 cgcttcccac gccacttctg cggcgcatta cttgcgcgag ttgttgccga atgcgagctt
     3721 ttggaatgta ccgccaacgg agcagaccac ccgtgaagtt tgcgatagga tactggaatt
     3781 ttctcgccaa ctctcatgag ccgttaggat cgtcgcggat aaattcgagt ttgacctcat
     3841 gcggatggac cgatatagaa aatcttgtag aagactccgt ttcatccgat aaaattgttc
     3901 caggaggctc gtcatggacg ttactacatc aactcttcaa ctcagtaccg ctgatggcaa
     3961 catggacgca ttcgaagcgc ggcccaagga tggcgggtcg tatcccggca ttgttgttct
     4021 gatggaagct ttcggtctta acgatcacat caagaaagtg accgagcgga ttgcccaaga
     4081 aggctacgtc gcgattgccc cggatctcta tcaccgtgag tccgagcgca tcattcccta
     4141 cagtgatatg aaaaaggcaa tcggtactat gaaccgcttg caggatccca aagtgatgga
     4201 ggatgtgggc gcggcgatcg cgcatttgaa gagccaaagc aacgccaaat ctggcgcgat
     4261 cggcgtcacc ggtttttgta tgggcggccg ttatacttat cttgcggcgg cgcatcacaa
     4321 tcaggacgtt aaagctgcgg tgtgcttcta cggtggtggc atccccatgg gggacccaag
     4381 cccgttgtcg cgcacgggtg aaatcacctg tccgatttat ctcttttttg gagccaaaga
     4441 tccgctgatc ccgatggatc aagtagcgca gatcaacact gagctgaccc ataagaaagt
     4501 gagcttctca atgaaggtgt atccggaggc aacgcacggg ttcttctgcg atgagcggtc
     4561 gagctaccat gagagcgccg ctaaagattc gtgggaaaaa atgaaaacat tttttgctca
     4621 acatttaaag tagcgaagag ccaagcgcgg ccgaagacgc ccgggtccgc ttcgctcata
     4681 gtaaaactct taatagagcc tttgtactta ttgaagcgat gcctggaaac aaaaacaaag
     4741 atatcgtaac tcttggcttg gtgcagatga gcgccagtga cgacaccgaa gccaatctcg
     4801 caaaggctga ggcgcgcata cgcgaggcgg caaaaagagg cgcccagatt gtctgcctgc
     4861 aggagttgtt tcgttcacgc tatttttgtc agagcgaaga tcaacgatgc ttcaacctgg
     4921 ctgaagccat tcccggaccg agcacggatc gtttaagcgc tttagcacgc gaactgcagg
     4981 ttgttatcgt cgcctcgctc tttgagaagc gctcggccgg catctatcac aacaccgcgg
     5041 tggttctcga tgccgatggc tcaattgccg gaaagtatcg caagatgcac attccggatg
     5101 atcccctcta ttttgaaaaa ttttacttca caccgggtga tctgggtttt ccaagctttc
     5161 agacccgtta tgcgagagtc ggcgctctgg tttgctggga tcagtggttt ccagaagccg
     5221 cccgcctggc gtcattgtcc ggagcggaga tactggttta cccaacagcg atcggctgga
     5281 tacccaagga gccgcgcgca gtggcgcaaa aacagcgtga cgcctgggag ctgatacagc
     5341 gcgcccacgc catcgccaat ggcgtctttg tggcttctgt caatcgcgtc ggcagggaag
     5401 gaagcttaaa gttctgggga cactcttttg tcgccggccc atctggagaa atcttgaacg
     5461 tcgctgctgc cgaccgcgaa gagattctca ttgctaaatg cgatctgcgc cagatcgacg
     5521 agatccgtga gagttggcct ttctttcggg accgacgcat tgacgcctac agttctttgc
     5581 agtcgcgatt catcgactcg cctgacggca cctagtcact catttcatgt ccgaccagag
     5641 caaaccctcg ccgatgccgc gcgacctggg gtattcgatg cccgcggaat gggtgccgca
     5701 ccgcgctacc tggctatcct ggccgcacaa tttggaaacc tggcctgctc agctcgagcg
     5761 cgtcagggag gcctgggttc agatgattct ggcgttgtcg ccgcacgaga aagtttgtct
     5821 tttgataaat gatgatttga gccgacagga tgtcaccctc cggctgaaaa cggccggtgc
     5881 ggtgatggaa aatgtcgccc ttatcaaaat ccccactgtc gacgtttgga tgcgcgacta
     5941 cggcccaacg ttccttgtgc gcaaccaagt ggatggtccg ttggcgatta acgactggat
     6001 cttcaatggc tggggcggca aatataaagc ttacgagcac gacgatcagg tcgccaagag
     6061 tatcgccgaa ttgctcggga tgccggtgtt tgcacatggg cttgtgcttg aaggtggcgc
     6121 catagaagtc aatggcgcgg gaatttgtct gaccaccgag cagtgtctgc tcaacactaa
     6181 tcgtaatccg gatctgagcc gaggggagat agagcaattt ctaagagact cgttgggggt
     6241 gagtcaaata atttggctag gcgagggagt tgcgggcgat gataccgacg gtcacatcga
     6301 tgatattgcc cggttcgtca atgccaggac catcgtctgt gctgccgaag acgacccgaa
     6361 agatgcgaat tacgctccgt tgcaagagaa tctcgaacgg ctcgagggag cacgcgatgc
     6421 tcttggtagc aggttcgatc tgttaacctt gccgtctccg cgccctgtga tttgccaggg
     6481 a
//
LOCUS       DASUTG010000378         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_653220, whole genome shotgun sequence.
ACCESSION   DASUTG010000378 DASUTG010000000
VERSION     DASUTG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653220"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..908
                     /locus_tag="VF089_08680"
     CDS             207..908
                     /locus_tag="VF089_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX6174077.1"
                     /translation="MLSKSRSGDPIIIPFADRRQAGKQLATRLAAYADRSDVIALALP
                     RGGVPVAFEVARALHAPLDVFLVRKLGVPGHEELAMGAIASGDVRVLNEEIINRIPIS
                     SSAIDEIAHREEEELKRRERLYRGNQAAPSVQGRTVILIDDGLATGSSMRAAATALRK
                     MQPSKIIVAVPVAAADTCADLRSEVDEVICGATPEPFMAVGAWYSDFEQTSDDEVREL
                     LNRIASHPPNSPLDS"
     gene            934..2266
                     /locus_tag="VF089_08685"
                     /pseudo
     CDS             934..2266
                     /locus_tag="VF089_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
     gene            complement(2684..2851)
                     /locus_tag="VF089_08690"
     CDS             complement(2684..2851)
                     /locus_tag="VF089_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174078.1"
                     /translation="MPKGKKRGKQPGPRKKTSKRKRQREISPQTHQPFEQDSKRRIGQ
                     YGGAGEPPIMK"
     gene            complement(2893..3459)
                     /locus_tag="VF089_08695"
     CDS             complement(2893..3459)
                     /locus_tag="VF089_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6174079.1"
                     /translation="MQLPLQVSFRKVRKTAAIEDLIRAQTAKLERVCDHIVGCKIAIE
                     KPQKHQRSGNPLRVRISVTVPPDHELVVTRESSEGDLHEPLPTVIRDAFSAMRRQLRK
                     LVEKQHGDVKHHPAQQVGGLIVRLFREQEYGFIKSPEGEEVYFHKNSLAGDDFYRLEV
                     GTGVQWHQEEGNNGPQASTVRIIDKPAK"
     gene            complement(3727..4104)
                     /locus_tag="VF089_08700"
     CDS             complement(3727..4104)
                     /locus_tag="VF089_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174080.1"
                     /translation="MRFQRHIDFHVIEHLKKVADELTSLAKTAPFALALGGTEETLEE
                     LLRNFPKPITKRIIGKFPVDYKHDTDQQTFERADLVWNHREQREEGHLVERVFDATKS
                     GMRGVLGTDSTLALWSKRRCAHW"
     gene            complement(4144..4521)
                     /locus_tag="VF089_08705"
     CDS             complement(4144..4521)
                     /locus_tag="VF089_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174081.1"
                     /translation="MTGKILIVEDDMITRRNLSVLLSDEGYDVDQAGDGVQALEILAR
                     RPFDLVLSDIVMPRMDGLKLLQQLQFMAPQIPVVIMTSYVSDSLSSVPSGAAEFIRKP
                     FVLDDLLFKVQRALDKAPQAPSV"
     gene            4864..>5619
                     /locus_tag="VF089_08710"
     CDS             4864..>5619
                     /locus_tag="VF089_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6174082.1"
                     /translation="MLRRIESLRKFSIGAKDGEIGRVVEFFFDDDKWTVRYLVADTGG
                     WLSGRKVLISPIFFGAVNWDSNVFNVNLTRQQLENSPSIDTEKPVSRQHESNFYDYYN
                     FPYYWLGTGLSSPATVSPAAAAPVPSGPEAAALGQQSDSEDIHLRSTKDIIGSYIETT
                     DGDLGHLEDFIIDDQNWMIRYLLIETTNWWPGKKVLISPEWVNDMVETDSRVYVDLTQ
                     EQIRTSPEYDETSVLDRDYESRLYQHYGRRRYWS"
BASE COUNT         1234 a         1581 c         1517 g         1287 t
ORIGIN      
        1 atcgttatcg aaaaagaaca tgtagatttt taagcaccgc gccgtctaaa ctcgccaggt
       61 cccttgttag aaattttcgc cagctcgttc ggcagcaagc atgccagtta ctcttggcat
      121 aggccttgcg ctatctttaa atcacaaccg ccgctacaaa acgactgtcc agattttttg
      181 ggaacagccg ttacggaggt gtcctcgtgc taagcaagtc tcgatcaggg gaccccatca
      241 ttattccttt tgccgatcgg cgtcaggcag gcaagcagct cgcgacccga ttagccgcat
      301 atgcggatcg ttcggacgtt atagcgctgg cgttgccacg cggtggagtg ccggtagcct
      361 ttgaagttgc ccgcgcgctt catgctcctt tggatgtttt cttggtgcgc aagcttggcg
      421 tcccagggca cgaagaatta gccatgggcg cgatcgccag cggtgatgtt cgcgtcctca
      481 atgaggagat cattaaccga attcctattt cgagttcagc aattgatgag atagcccatc
      541 gcgaggagga agagttgaag cgccgcgaac ggctgtaccg tggaaatcag gcggctccaa
      601 gcgtacaagg ccggacggtg attctcatcg acgatgggct ggcaaccgga tcaagcatgc
      661 gcgccgctgc gaccgcgctg cgaaagatgc agccgtccaa gatcatcgtt gcggtgcctg
      721 ttgccgctgc cgacacttgc gccgatttgc gcagcgaagt cgatgaggtg atttgcggcg
      781 ctacccccga gccgtttatg gctgtgggcg catggtattc ggatttcgag caaacaagcg
      841 atgacgaggt gcgcgagctg ctgaaccgaa tcgcctctca tccgccgaac tccccactcg
      901 attcgtgacc ggaactaaag ccaaggaagc tttatgcctc cccccgacga tgttattggc
      961 gcgctgaccg ctgccgctcg accgctggtc ggcgccagcg atgactacga ttacttcttc
     1021 gaactgatcg gcgatgcccg actggtcctg ttgggtgaag ccacgcatgg tactcatgag
     1081 ttttatcgtg agcgagcgca gatcacgaaa cggctgatcg ccgagaaagg cttcagcgct
     1141 gtggcggttg aagcggactg gccggatgcc taccgggtaa accgttacgt ccggggccaa
     1201 ggcactgcca ccgatgccga tgatgctcta gcaggtttca agcgtttccc gacctggatg
     1261 gcgcaacacc gatgttcttg agttcgtgaa ttggctgcgc gcctataacg acaggactcg
     1321 cgccgagatg aaagcgggtt tctatggtct tgatctatac agtttgtacg gctcgatggc
     1381 ggcggtgctg agttatttgg acaaggttga ttcggcagct gccaagagag cgcgttatcg
     1441 ttattcctgc ttcgatcagt tcggcgagga cacgcaggct tatggctatg ccgcaagctt
     1501 cggcctgacg gagtcctgcg aacgcgaagt ggtcaaccag ctccatgagc tacaacgtcg
     1561 cgcaaccgat tatgcaagcc gcgacgggcg tgttgccgca gatgatttct tctttgccga
     1621 acaaaacgcg cgcctcgtca aagatgccga acaatattat cgcgtgatgt ttcggggccg
     1681 cgagtcatct tggaatctcc gcgaccgtca catggctgat actttggagc atctgattgc
     1741 ctttcttact ctgcagggac cgcggccgaa ggtcgttgtc tgggaacaca actctcatat
     1801 cggcgacgct cgcgccactg agatgaccga ccgcggagaa gtcaacgtcg gacagcttgt
     1861 gcgcgagcgc tacaataatg aagcggtttt gatcggcttt accacgtatc agggcaccgt
     1921 gacggctgcg acagactggg atggccctgc tgaatgcaag cgcgtgaggc cggctcttga
     1981 aggcagctat gaggcgctgt ttcatagtgc cggtccaaag cggttcctgc ttgatctgcg
     2041 agaccagcgc gttcaatgct tgcgcgagcc ccgcctggag cgagccatag gcgtcgttta
     2101 ccgcccggag agcgagcgag ttagccacta tttcaaagcc aatttgccgg cgcaattcga
     2161 cttggttttg catttcgatg aaacacgcgc gctgcagcca ctggagcctt cgacagattg
     2221 ggaaagtggc aaagaagcgg ctgagaccta cccgtcgggt ttttgacctc aatcgcgaag
     2281 gaggaagtta tgctaccgga actcagtatc ttcccggtgg ggcgaagccg catttccagc
     2341 ggcgaactcg ccaagtgctc aaagtggtag agaagtccgg cttgccctga tcagctcaca
     2401 ccctcagcaa cttgcatcga aggtgaatgg gaccagattg tgccgctaat tgtgcaatat
     2461 cttgagcgcc ccgcaaagac tcagctcaga tggtcacatc tatcaagctc tgagaggatg
     2521 atggcgaagg gacagaacag cccctagacc gatcgtgctg attattttaa agcggttagt
     2581 gtccagatca tttttggtca tgatccacct cccgcaaaaa ctcccaatct gttacgcctt
     2641 tcaacttggc catccccgat acaaccgatc cccggaatcg cgatcacttc atgatcggcg
     2701 gctcgcctgc tccgccatat tgcccaatgc ggcgcttgga atcctgctca aacggctgat
     2761 gggtttgtgg actgatctca cgctgtcttt tacgtttcga cgttttcttc cgcggccctg
     2821 gctgctttcc tcttttcttt ccctttggca tacagtctcc tcttgcgatt tagagctgac
     2881 gacgtctgac attcatttag ccggcttatc gatgatccgc acggtactgg cctgcggacc
     2941 attgtttccc tcttcctggt gccactgcac tccggtaccg acttctaagc ggtagaaatc
     3001 atcacccgcc aaactgttct tgtgaaagta aacttcttcg ccttccgggc tcttgatgaa
     3061 tccgtattct tgttcgcgaa ataaccggac gatcagccct ccgacctgct gcgctggatg
     3121 atgtttcaca tctccgtgtt gcttctcgac caatttgcgc aactggcggc gcatcgcgct
     3181 aaaggcatcg cgtatgactg ttggcaatgg ttcgtgaaga tcgccttcgc tggactcgcg
     3241 cgtcacgacc agctcatgat ccggcggaac cgtcacgctt atgcggacgc ggaggggatt
     3301 gccgcttctt tgatgtttct gaggtttctc tatggcgatc ttgcagccaa caatgtgatc
     3361 gcaaacccgc tcgagcttag cggtttgagc acggataaga tcctcgatcg cggcggtctt
     3421 gcgcacctta cggaaactta cttgaagagg caactgcatc tcccacgtct ccttgcgatg
     3481 aagctcgggt gaatctaata cctcagcagc gcgccgagac cgccctcctc aagcagacga
     3541 ttgcgcgcct cgtgggcgga cacaacttcc gcatccgctc cgtgagaatt gcctttttaa
     3601 cggcacgatc ggtaacgtct cccagctcgg ccgttgcccc gcaaagcggg caactttcaa
     3661 actcctgggc agagaaatag tcgcatcgag tgcacaccga tccatctatt gccagtccgt
     3721 ctgcgatcac caatgtgcgc accttctctt cgaccagagc gccagggtcg aatcggtccc
     3781 cagaacgcct cgcattcccg atttggtcgc gtcaaagacc cgctcaacca aatgtccttc
     3841 ttcacgctgc tcccgatggt tccatacgag atcggcgcgt tcgaaagttt gttgatcggt
     3901 atcgtgctta taatcgacag ggaattttcc aataatccgt ttggtaatcg gcttaggaaa
     3961 atttctgagt aactcctcga gggtttcctc ggtgccgccc agcgccaagg caaatggagc
     4021 ggttttggcg aggctcgtga gctcgtcggc aactttcttt aggtgctcga tcacatggaa
     4081 atcgatgtgg cgctgaaagc gcatctaaca acgcccgccc tactatgcag ccacatgtta
     4141 gattcacaca ctcggagcct gcggtgcttt atccaacgcg cgctgcactt tgaacagaag
     4201 atcgtccaag acgaacggct ttcgtatgaa ctcggccgct cctgacggca cactcgaaag
     4261 cgaatccgaa acatagctcg tcatgatcac tacgggtatc tgtggcgcca taaactgaag
     4321 ctgctgcagc agcttcaggc cgtccatccg aggcatgacg atatccgaca acaccagatc
     4381 aaacggccgc cgggcgagga tttcaagcgc ctgaactccg tcacctgcct gatctacgtc
     4441 atagccctca tcgctcagca gcactgaaag atttcttcgg gtgatcatat cgtcttcgac
     4501 gatgagaatt ttgcccgtca tagcccaagt acccccgcaa aaacggttcc agacccacct
     4561 attcacgcgc tgagcatcgg agaccggaca agagcggcgg agaggttgtt gttgaaccaa
     4621 tcaatacgcc atcttttctg ttcaacagtc gacgatctac tctagcggtg gtaaagggct
     4681 atcaacctcc acctctggag gctgtgatcc gtcgcacttt cgacacacag gatattccat
     4741 gcgtgcccca cctgagggcc accttcggca gcggatgcag cgccggcccg ggtaaaaggc
     4801 ttcaggtggc cgtggcatct tcgttgcaca gaacgagtta cgccgcgtgc ggacaaggaa
     4861 agcatgctga gaaggataga gagtctgagg aaattttcga tcggcgctaa ggatggcgag
     4921 atcggcaggg tagtggaatt tttcttcgac gatgacaagt ggaccgtcag gtacctcgtt
     4981 gccgataccg gaggttggct aagcggcagg aaagtgctga tctcgccgat cttcttcggc
     5041 gcggtgaatt gggacagcaa cgttttcaac gtcaacctta cccgacaaca actcgaaaat
     5101 agcccgagca tcgacacgga aaagccagtg tcgcggcaac atgaaagcaa tttttacgat
     5161 tactacaact ttccctatta ctggctgggt acgggactct cgagtccggc gacagttagt
     5221 cccgctgcag ctgcaccagt cccaagcggc ccggaagctg ccgcattggg tcagcaatcc
     5281 gattccgaag acattcatct gcgtagcacc aaagacataa tcggttccta catagaaacc
     5341 accgatggtg atctcggcca tttggaggat ttcatcatcg atgatcaaaa ctggatgatt
     5401 cgttatctac tcattgagac gaccaattgg tggccgggca agaaagtgct gatctcaccg
     5461 gagtgggtca atgacatggt cgagaccgac tccagagttt acgtcgatct tacccaggaa
     5521 cagataagaa ccagccccga atacgacgag accagcgttc tcgatcgtga ctacgagtcg
     5581 cgtctctatc aacattacgg ccggcggcgc tactggagc
//
LOCUS       DASUTG010000379         6900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_60983, whole genome shotgun sequence.
ACCESSION   DASUTG010000379 DASUTG010000000
VERSION     DASUTG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60983"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF089_08715"
     CDS             <1..307
                     /locus_tag="VF089_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174083.1"
                     /translation="MLEATQINDYQEALIELYGDMDGSNKPAAPLEPATEESLERYVL
                     EKINIKEEDLHELGRVRAEIVKQQLVEGGVDPERLSALAPENITSDPKPAVEIQLVS"
     gene            506..1300
                     /locus_tag="VF089_08720"
     CDS             506..1300
                     /locus_tag="VF089_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX6174084.1"
                     /translation="MRKPTTPETQAVESERSELLQRIADWLELPMLVLALAWLALLIW
                     ELTWGLSPLLETAGIVIWIIFILNFAIEFSLAPRKLNYLKSNWLTAVSLIVPALRALR
                     ILRIVRVLSATRGLRLIKVIGSLNRGTRALGKSFGRRGFGYVVALTAVVTLLGGAGMY
                     AFENEEGGLESYGEALWWTAMIMTTMGSQYWPQTAEGRLLCVVLALYAFAVFGYVTAT
                     IATFFVERDAESAESEVASAASIAQIRTEITALRSEIAALLTQKDH"
     gene            1795..2826
                     /locus_tag="VF089_08725"
     CDS             1795..2826
                     /locus_tag="VF089_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174085.1"
                     /translation="MRKPFRGGFLLLSLITAFGFPAKALAQKLTVGWSAVSALNSPFW
                     VAKEAGFLEKEGLDANLVYIPSSSTMAQSQISGDVQISTANSQVIVDADLRGGDLVAM
                     GAVTNAVAFYVMANPAISSVADLKGKRVGVTRFGASTDFAIRTLLEKHGLKPITDVPI
                     VQIGGMPEIAAALSNGAIAAAPMSYPMAYVAEQNGVKLLANMAKEGIAFVHVGITTSR
                     GFISGRRSQAKAFLRAYSHAVHYMHTNPDGFKKIITRYSKITDPGMLQGTVQYAYDFI
                     EKIPLVERDAFQNTIDEIAARRPEAKQAKPQQFYDNSLVQELIKEGFFKSLWGADASA
                     AALRSGDRF"
     gene            3440..3949
                     /locus_tag="VF089_08730"
     CDS             3440..3949
                     /locus_tag="VF089_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008958220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX6174086.1"
                     /translation="MNLFFSGWPEIWRTVILGLLSYASLVLFLRISGKRTLSKMNAFD
                     LVVTVALGSTLATILLNKQVALAEGLVALLLLIGLQFCVTWGAVRSSRLESLVKNEPA
                     LLLHQGQFLRRPMLQERVTESEIRSAVHGRGFADIEPGLSVILETDGTFSVVVHRPED
                     QSLDGREAT"
     gene            4208..4426
                     /locus_tag="VF089_08735"
     CDS             4208..4426
                     /locus_tag="VF089_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174087.1"
                     /translation="MVALLAFAVTLVIAVYVSGIAQGTVLSIGIANAGRIFHWIARVL
                     TASSIVHSSTEVLLSRRLYDQQSEGARA"
     gene            4431..5405
                     /locus_tag="VF089_08740"
     CDS             4431..5405
                     /locus_tag="VF089_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6174088.1"
                     /translation="MAESKKAIFAAIAGNFAIAVTKFTAAAFTGSSAMISEGIHSLVD
                     TGNGGLLLLGIRMSKKPADTTHPFGYGKELYFWTLIVAMLIFGVGGGVSIYEGILHLL
                     HPSPLQDPTWNYVVLGLAMIFEAIVFVIALREFQASKGRQSIWQAVRTSKDPTTFTVL
                     FEDSAAMLGLIAAVVGIFLGHYFENPYLDGAASIVIGLILTAVAFFIGYESKGLLIGE
                     GADPAVLRSIKRLAESDPKITTVERPLTMYFGPHTVLLTMDVEFRDKLSGDERDAAVQ
                     RLEKAIHEKYHDINHIFIEAKSLSRKTPGEPPLTGDSARSTKPPPGGD"
     gene            5441..5752
                     /locus_tag="VF089_08745"
     CDS             5441..5752
                     /locus_tag="VF089_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174089.1"
                     /translation="MKPKVYYRLTNNWLERAVRFIVEDHGIRRVKDAMSRDILRALKE
                     ADIKVASTTFEITGLPPLRIRDAAQPLDKRMDNNRAGTKRAIARLQFGSIEIQNRNEA
                     R"
     gene            5749..6756
                     /locus_tag="VF089_08750"
     CDS             5749..6756
                     /locus_tag="VF089_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6174090.1"
                     /translation="MNKSEVVVITGASAGVGRATARAFAQRRAKIGLLARGRDGLEGV
                     RREIERAGGEALVLPTDVASSEQVEAAAQAVEDKFGAIDIWINNAMVSVFSPIKEMTP
                     EEFKRVTDVTYLGAVYGTLSALRRMLPRDRGVIVEVGSALAYRGIPLQAAYCGAKHAM
                     QGFVDSLRSELLHDQSRVRVTMVQLPALNTPQFSWVKSRLPRKPQPVPPIFQPEVAAD
                     AILWAALHHRPEVYVGWPTVKAIVGNKIAPRFADWYLSRQGYEAQQTDEPVEAGRVDN
                     LWEPVPGDHGAHGDFDARSLPSSWQFRLSKHKTSFALAGLTIAAALLALAKDTRRVRL
                     E"
BASE COUNT         1532 a         1803 c         2001 g         1564 t
ORIGIN      
        1 gatgctcgag gcgacacaga taaatgatta tcaggaagct ctcatcgagc tctacggcga
       61 tatggacggt tcaaacaaac cggcggcgcc gctcgagccg gcgactgaag agtcattgga
      121 gcgttacgtc ttggagaaga taaacatcaa agaggaggat ctgcatgagt tgggacgtgt
      181 gcgtgctgag atcgtgaagc aacagcttgt cgaaggcgga gtagaccccg aaaggctttc
      241 ggcgctcgcg cctgagaata tcacgagcga tccgaagccg gctgtagaaa tccagctcgt
      301 ttcctgagga aggcacaagt ttcggccgat gatcctgaaa cgcattcagc ccaagtgaaa
      361 aggaacgctg gcatagcacg aagattctga gaatcacttc tccgacgcgg tagccacgat
      421 ggtaggcgtg cacaggtgaa gcctgcatgg agattcgttt cgttcatgcg gaatgaggtg
      481 gatggcctga cttttctaaa cttcaatgag gaaaccaacc acacctgaaa ctcaggccgt
      541 cgagagtgag cgcagcgagc tgctgcaaag gattgcagac tggctcgaac tgcctatgct
      601 cgtgctcgct ttggcttggc tggcgttatt gatttgggaa ttgacctggg ggttgagtcc
      661 gctgcttgaa accgccggca tcgtcatttg gatcattttc attttgaatt ttgcgattga
      721 gtttagcctc gctccgcgca agcttaacta tctcaagagt aactggttga cggcggtttc
      781 cctgatcgtg ccggcgctgc gcgcgttacg cattcttcgg atcgtgcgcg tgctgtctgc
      841 gactcggggt cttaggttga tcaaggtcat aggatcactc aaccgaggca cgcgcgctct
      901 cggtaaaagc ttcggcaggc gcggcttcgg atacgtcgtg gcgctcacgg cggtggtcac
      961 gctactgggc ggagcaggga tgtacgcgtt tgaaaacgaa gaaggcgggc tggagagcta
     1021 tggagaagcg ctgtggtgga cggcgatgat catgacgacc atgggctcgc agtactggcc
     1081 gcagacggct gagggtcgcc tgctttgcgt cgtcctggcc ctctatgcgt ttgccgtatt
     1141 cggctatgta acggcgacga tcgcgacctt ctttgtcgag cgtgacgcgg aaagcgctga
     1201 gtcggaggtg gccagcgcag cgtcgatcgc acagataaga accgagatta ccgcgttgcg
     1261 ctcggaaatc gccgcgctat tgacgcagaa ggatcattga aaattttagg atccagaccg
     1321 aagagtgaaa atcgtcagct cgcgccggaa cgaaccacaa gctcgaagaa actgcctccc
     1381 atagttggat taaccgtgcc ttcatgattt cggtccaagc tatatgcttc tgggttcgtt
     1441 accggcttct ctgacgtgtg acatacaacc atgccgttta cctgcgccct agtgcaacga
     1501 ctgattcttc ctcatagatt aaagctgccg ggagcgccga tgcaaatcag caactgaaat
     1561 gctagccagg agatctcttg tttagcgccc gccatatgac tctcgcgcgc ctgtgcagat
     1621 cggttacaca acgtaaattg gcaggacgtt ttactgcaca ggaacaaggc tgagcttctt
     1681 cggcatagtt gctcgatcgc agactttgct ggggtcgcag ttctgaaact tcttgccgaa
     1741 atttggtatg aactttgccc acagacactg tagcgttgag ggagggatat cgctatgaga
     1801 aagccatttc gcggtggttt tttgttgtta tcactcatca cagccttcgg ctttcctgct
     1861 aaggcgctgg cgcaaaagct aacggttggt tggtcggcgg tgagcgctct caattcgccg
     1921 ttttgggtag cgaaagaagc aggtttcctc gaaaaagaag gactcgatgc aaacctggtt
     1981 tacattccaa gctcatcgac catggcgcag tcgcaaattt cgggtgacgt ccagatctca
     2041 acggccaata gccaggttat cgtggacgcg gatctgaggg gtggtgatct ggtcgcgatg
     2101 ggggcggtga ccaatgcagt ggccttttat gtcatggcga atccggcgat ctcaagcgtc
     2161 gcagacctca aaggaaaaag agttggtgtt acccgtttcg gcgcgtccac ggatttcgcg
     2221 atccgcaccc ttctggagaa gcacggtctc aagccgatta ccgatgtgcc gatcgtgcag
     2281 atcggcggca tgccggagat cgccgcagcg ctgtccaatg gcgctattgc agctgcgccc
     2341 atgtcctatc ctatggctta cgtcgcagag caaaatggcg tgaagcttct cgccaacatg
     2401 gccaaggagg ggatcgcctt tgttcatgtg ggcatcacca cttcgcgcgg tttcataagc
     2461 gggcgacgat cgcaggccaa agcgtttttg cgcgcgtaca gtcatgcggt gcactacatg
     2521 cataccaatc cagacggctt caagaagatt atcactcgct actcaaagat taccgatcct
     2581 ggaatgcttc aaggcaccgt gcagtatgct tacgatttca ttgaaaagat ccctttggtg
     2641 gaacgtgatg cttttcaaaa taccatcgac gagattgcag ccaggcgacc cgaggccaaa
     2701 caagcaaagc cccagcagtt ctatgacaat tccttggtgc aggagttaat taaggagggc
     2761 ttcttcaaat ctctttgggg cgccgacgct tcggcggcag cgcttcggtc cggtgatcga
     2821 ttctaagagc tctggctaac tgcgagaagc cgaaaggtgt tctgccctga caagttgtac
     2881 ccgggccgaa gacgctcggc ctggtttatc gtcgccgggc gccaagcgat accggcggtt
     2941 ctatggcgag gggtggcggc ggcgacacca tgatgcaatt ggctgggttg ttctcaaccg
     3001 cagagatgat ccggcatatc ccggcagtat ctgcgaggtg gttcgggctg gcgaagagga
     3061 agcgccgtgc tagttttcct actggtgcga tggcaggccc gacaggccgc agaacgagaa
     3121 cccctgacaa ctcgttaaag agactgcgct tgccatgcgc aagacccgcc gaaggatccg
     3181 accggaggcg ctctttattt ccactccgcc gagatgacgg taccgtggaa gaggcatcga
     3241 cgacgcacgg tgccaatcgg caatcaaatc ttctatcgct aatcattcgg caatgaagcc
     3301 ggcttgagcg tgctgaattc taagccgcgc aagcatgtgg tacatcgttt gcgctcatcc
     3361 tccggatcgc ttagccgttc agaggtgagc gcagaccgat cttgatgtga tccaaaaacg
     3421 aagaacgtga gacaattgaa tgaatctttt cttttccggc tggccagaaa tttggcgcac
     3481 cgtcatcctt gggttattat cgtacgcttc tcttgtactg ttcttgcgaa tttccggcaa
     3541 gcgcaccctg tcaaagatga acgcgttcga cttagtagtg accgtggcgc tcggatcgac
     3601 gctggcgacc attctgttaa acaagcaggt cgcgctggca gaagggctgg tggcattgct
     3661 gcttctgatt ggcttgcagt tttgcgtcac ctggggagct gtccggtctt cacgtttgga
     3721 atccctggta aaaaacgagc cggccctgct gctacatcag ggccagtttc tacgccggcc
     3781 aatgcttcag gaacgggtta cagaatctga aattcgctcc gcagtccatg gccgaggctt
     3841 cgccgatatc gaaccgggat tgtctgtcat tctcgaaacc gacggcacat tcagcgtcgt
     3901 tgttcatcga ccggaagatc agtcgctcga tggccgtgaa gcgacgtgat cgatggccgt
     3961 gaagcgatct gatcaagaag cggccttgct gtgaccgcgt gcttcagcga agtccggatg
     4021 cccgatcgcc tcattcttga gacacgacag cgcggctgca ttcggaattg tgggacgtag
     4081 ctcaaagcat ctgcgctacc cggttatctg agggagcagc gggaacatgc tcggcatgac
     4141 gcttgcgttt agcgcagtgt cgcgctcaca caacgacttt ccgcatctct gaggaattta
     4201 cttttacatg gtcgccctct tggccttcgc tgttactttg gtaatcgccg tctatgtttc
     4261 gggaatagcg cagggaactg ttttatcgat cggcattgcc aacgccggta gaatttttca
     4321 ctggatagcg cgcgtcctga ctgcttcctc tatcgttcat tcctcgaccg aggtcttgct
     4381 aagccgccgg ctctacgatc aacaatcgga gggggcacga gcctgaaatt atggcggagt
     4441 cgaaaaaagc cattttcgcg gcgatagccg gtaattttgc catagcggtc acgaagttta
     4501 ccgccgccgc attcaccggc agttcggcga tgatttcgga aggcattcac tcgctcgtcg
     4561 ataccggtaa cggcggatta ctgctgcttg ggatacgcat gagcaagaaa ccagccgata
     4621 cgacccatcc gttcggctac ggcaaggagc tgtacttctg gaccttgatc gtggccatgc
     4681 tgatctttgg tgttggcggc ggcgtaagca tttacgaggg gatcttgcat ttgttgcacc
     4741 caagcccgtt gcaagatccc acctggaatt acgtggtatt gggtcttgct atgatctttg
     4801 aagcgattgt tttcgttatc gctctcaggg agttccaggc tagcaaaggt cgacagagca
     4861 tttggcaggc cgtccgcacg agcaaagatc cgacgacgtt cacagtgctc ttcgaagact
     4921 ctgccgcaat gctggggctg atcgcggcag ttgttggaat ttttcttgga cattacttcg
     4981 agaatccgta tctcgatggc gccgcatcca tcgtcatcgg actgattctt acagccgtag
     5041 ctttcttcat cggctatgag agcaaaggtc tcctgatcgg cgaaggtgcc gatcccgctg
     5101 tcttacgaag cataaagaga ttggcggaat cggatccaaa aataacaacg gtcgagcgtc
     5161 cgctgacgat gtatttcggg ccgcatacgg tgctgctgac gatggatgtc gagtttcgtg
     5221 acaaattatc gggagacgaa agagatgcgg cggtgcaacg cctggaaaag gcgatccacg
     5281 aaaaatacca cgacatcaat catatcttca ttgaggcaaa atcgctgtcc aggaagacgc
     5341 ctggagagcc gccattgact ggcgattcgg cacggagcac taagcccccg ccaggcggag
     5401 actaagcgaa actctgatgc tatttcgtta aacccgccac atgaagccaa aggtttatta
     5461 ccgcctaacc aacaattggc tggagcgtgc cgtccgcttc atcgtcgaag atcatggcat
     5521 ccgccgtgtg aaagatgcaa tgagccgcga tattctgcgt gcattgaaag aggcggacat
     5581 caaggtcgca tcaacaacat ttgaaataac cggtttgccg ccgctgcgca ttcgcgatgc
     5641 agctcagcca ctcgataagc gtatggacaa caacagggcg ggcaccaagc gagcaatagc
     5701 tagactgcaa ttcggaagca tcgaaatcca aaacagaaac gaggcaagat gaataaatct
     5761 gaagtggtcg tcattaccgg ggcatcggcg ggagtgggtc gggcaacggc gcgcgccttc
     5821 gctcaacgaa gagcgaaaat tggattacta gcgcgcggcc gagatggctt ggaaggcgtg
     5881 cgccgggaaa tcgagagagc aggcggcgag gctttggtgt tgcccaccga cgttgccagt
     5941 tcggagcagg ttgaagctgc agcacaagcc gtggaagaca agttcggggc catagatatc
     6001 tggatcaaca acgccatggt ttcagttttt tcgccgatta aagagatgac tccagaggaa
     6061 tttaaacggg tcacggatgt cacttatctt ggcgctgtat atggaacgtt atcggcgctg
     6121 cggcgcatgc tgccgcgcga ccgcggggtt atcgtggaag tcggctccgc tttggcgtat
     6181 cgcggtatcc cactgcaggc tgcttactgt ggcgccaagc acgcgatgca gggattcgtc
     6241 gattccttgc gcagcgagct actgcacgat cagagccgcg tccgggtaac catggttcag
     6301 ttgccggcgc tcaacacgcc gcagttctcg tgggtcaaaa gccggctgcc gcgcaaaccg
     6361 caaccggtgc cgccgatctt tcagcctgaa gtggccgctg atgcgatcct ctgggcagcg
     6421 cttcatcatc ggcccgaggt ttacgttggc tggccgacgg tcaaggccat tgttggcaat
     6481 aagattgcgc cgcgctttgc cgactggtat ctgtcacggc agggctatga agcgcaacaa
     6541 accgacgagc ccgtcgaagc ggggcgcgtc gacaatttat gggagccggt tcccggcgat
     6601 catggtgcgc atggagattt cgacgcccgc tcgctgcctt caagttggca gttccggctc
     6661 tccaagcata agaccagctt cgctttagca gggctgacga ttgccgccgc tctactggcg
     6721 cttgctaaag acacgcgccg agtccgcctg gaatagccga gccgtgtctc ggactacttt
     6781 ggagagatct atgaaacgaa ttttactaat aagcctactt gcttccttct tgtcggcttg
     6841 cgaaaagcag gaagccaaag ctccggcgcc agaggctcct ccggcgccat cggccgctga
//
LOCUS       DASUTG010000380         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_950727, whole genome shotgun sequence.
ACCESSION   DASUTG010000380 DASUTG010000000
VERSION     DASUTG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950727"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..642
                     /locus_tag="VF089_08755"
                     /pseudo
     CDS             220..642
                     /locus_tag="VF089_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
     gene            684..1238
                     /locus_tag="VF089_08760"
     CDS             684..1238
                     /locus_tag="VF089_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX6174091.1"
                     /translation="MVYYISNLVTWLLESPEGRAEAQAENNHGTWYDVQTASFGLFTD
                     KRDFAQRVLSDVRRKRIARQIEPNGGQPLELARADSWSYSLFNLEALFDAAALGDHLA
                     MDLWNFETADNRSIRKALAWLIPFATGEKRWPLRQQSRWHPERLAPFLQRAALRYREP
                     SYVTALGKLAAPSIDSRERLLYPE"
     gene            complement(1381..2358)
                     /locus_tag="VF089_08765"
     CDS             complement(1381..2358)
                     /locus_tag="VF089_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174092.1"
                     /translation="MIRVLLLSLVLLLPASSRAQEKFIASYAGFAGFQAPLWAAKDFG
                     FLAKYGVDTDLVMIPGSARGAQALLGGSIHFGQIDGTALISAINQGADLVFVASSLNR
                     FPFSLVTQKNIRQPKDLVGKKVGIVSLGGAHEVSLILALREWNIPRASVTLLASGPAA
                     NRLVALASGALDATLLAPPETGEAARMGLLTLAHMTELKAAYFPMNAIATRRSLLEKN
                     REAVKRFLQAYSEGIHHFMTRKDKSLVLLTQRMKQKNPAVVEETYQYFASTFSSPPRM
                     SQEGMRAAIDMLAQRSPEIKFDTNLSRYLDERLLDELEREGFFTELAKK"
     gene            2564..>3175
                     /locus_tag="VF089_08770"
     CDS             2564..>3175
                     /locus_tag="VF089_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6174093.1"
                     /translation="MSTNRTDVPSLDSSELKELKEKLITACHILDHEGVTDGYGHISV
                     RVPGADAFITISNVSPGCVTEDRLIMQDLTGNYLGGAETPPNEWPIHACILRARPDVE
                     SIAHTHSKWSTIFSVLQAKLKPMHHYGKFLSADGPPVYGGIGLIRTMERGNDLAQALG
                     DGPVVLMRAHGDTVVGESVEQVIERTTRLAMLGEWNHLALLHGE"
BASE COUNT          724 a          904 c          859 g          688 t
ORIGIN      
        1 gtctcggtga atctcctcgc cgctctgctg acaatctctt ttggcatttt tttgagcgat
       61 ctgatggtcc cgggtccgag ctttgcgaca aatgctctac cccgcgtttt catcctcgat
      121 cgtgtgggtc tgacaatcgc aaagcaacgg acgcagcaaa acgacgaaag tctcaaaccc
      181 gcaatggcaa agcttaggcg cgatgccgag cgcgctctca tgagcgcgcc gctctgcgta
      241 acccgcaagg acagtgcacc gcccagcggc gataaacacg attacatgag tcttgcaccg
      301 tactggtggc ctaacccgaa cacccacaac ggccttccat acgtccgccg tgacggagaa
      361 atcaatccgg agcgtaacaa catccccgat cggcaaaatc tagagaagac ggtgactttg
      421 gtaaaaacac tggcgcttgc ctactatttc acggcaaacg aggactacgc caaacaagcc
      481 acgaccatgc tccacgtttg gtttttcgat ccgtcgacga gaatgaatcc gcatttgaaa
      541 tatgcacagg gcatcccggg gcgcaatgcc gggcgcgctg cgggtattat cgagagccac
      601 aacttcccag atctcatcga cgccgtgggt ctgttagctg gatcagcaac ctggacccag
      661 gcacatcagc ggcagctaca atcgtggttt actacatttc taacctggtt acctggttat
      721 tggagagccc cgaaggtcgc gctgaagcgc aggctgaaaa caatcacggc acatggtatg
      781 acgttcaaac tgcgtcattc gggcttttca cggacaagag agatttcgct caacgggtgc
      841 tcagtgatgt tcggcgcaag cgaatcgcca ggcaaatcga gcccaatggc ggacagccgc
      901 tcgaacttgc gcgcgctgac tcttggtctt attcgctgtt taacctcgag gcactatttg
      961 acgcggctgc tctgggcgac catttagcga tggatctatg gaacttcgag actgcggata
     1021 accggagtat ccgcaaagca ctggcttggt tgattccgtt tgccaccggt gaaaagcgct
     1081 ggccgttacg ccagcaatct cgctggcatc ccgagcgcct tgcgcctttt cttcaacggg
     1141 cggcgctgcg ctatcgggag cccagctatg taactgcgct cggaaagcta gcggcgccat
     1201 ctatcgatag tcgcgagcgc ctgctgtatc cggagtgaga gagcgcattc gaaggcaagg
     1261 tagaatgatt tgatgttgac cagttaagcc aggggagtgg acgcataaac acacatgcac
     1321 gcgggcagcc gagccagccg atcgaaaata atccttcaac cgcaaaatac aggcgcccgg
     1381 ttattttttt gctaattccg tgaaaaaacc ttctctctcc aattcgtcca gcaagcgctc
     1441 gtctagatat ctgctcaggt tggtatcaaa tttgatttcc ggagaacgtt gagccagcat
     1501 atcgattgcg gcgcgcatgc cttcctgtga cattcgcggc ggcgaggaaa acgtcgaagc
     1561 aaagtactga taagtttcct ccacgaccgc aggatttttt tgtttcatcc tttgcgtcaa
     1621 gagcaccaag gatttgtctt ttcgggtcat gaaatggtgt attccctcgc tatacgcctg
     1681 caggaatcgt ttcaccgctt cccggttttt ttcaagcaac gagcgccgcg tcgcgatggc
     1741 gttcatgggg aagtaagccg ctttgagctc ggtcatatga gccaaggtca acaagcccat
     1801 gcgcgcggcc tcgccggtct cgggcggcgc caacagagtg gcgtcgagag cgccgcttgc
     1861 cagggcgacg agacgattcg ccgccgggcc gctggccagc agggtcaccg aggcgcgcgg
     1921 gatattccat tctctaagcg ctagaatcag cgatacttcg tgcgcgccgc ctagactcac
     1981 gatacccact tttttcccga ccaggtcctt gggctggcgg atgtttttct gggttaccag
     2041 actgaatgga aatcgattca gggatgaggc gacgaacacc aggtcggcgc cttgattgat
     2101 tgcggagatc agcgccgtcc cgtcgatctg accaaagtgg atgctgccgc cgagcaacgc
     2161 ttgtgctccc cgcgccgaac cgggaatcat gacgagatcg gtatcaacgc catactttgc
     2221 gaggaagcca aaatcctttg cggcccaaag aggcgcctga aatccggcaa atcccgcata
     2281 gctggcgata aacttctcct gagcgcggct ggacgccggc agtaacagca ccagactcag
     2341 aagcaagaca cgaatcattg gcacctcctc gatttcaacc gacgagcctc attttgaatg
     2401 aactcccctt gccgaacact ccgggtcaag cgagctgtcg cgagttatcc aattctcggc
     2461 agttatttgt caagtgaagc ttgacaggtg gactggcggc gcaatagatt tcctccacaa
     2521 ccgctgatcg ccgctttgtt gatgattagc gtaaggagat ttcatgagca caaatcgcac
     2581 cgatgtaccc agcctcgatt cctccgagct caaagaattg aaggagaagc tcatcaccgc
     2641 ctgtcacatt ctcgatcacg agggcgtgac cgatggctac ggtcacatca gcgtgcgcgt
     2701 ccccggcgca gatgctttta ttaccatttc caacgttagc cctggctgcg ttactgagga
     2761 ccggctgatc atgcaagact tgaccggcaa ttatctcggc ggcgcagaaa cgccgcccaa
     2821 cgaatggccg attcatgcct gcattctaag agcgcgtccc gatgtcgaga gcatcgcgca
     2881 cacacactcg aagtggagca caatcttcag cgtgttgcag gccaagctta aacccatgca
     2941 ccactacggt aaatttttat ccgccgatgg gccgccggta tatggcggca ttggcttgat
     3001 tcgtactatg gagcgaggca atgatctagc ccaagccttg ggcgacggcc cggtggtgct
     3061 aatgcgcgcg cacggggata ccgtggttgg cgagtccgtc gaacaggtaa tcgaacgcac
     3121 cacccgcctt gcaatgcttg gcgaatggaa tcatctggcg ctgctccatg gtgaa
//
LOCUS       DASUTG010000381         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_209388, whole genome shotgun sequence.
ACCESSION   DASUTG010000381 DASUTG010000000
VERSION     DASUTG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209388"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF089_08775"
     CDS             <1..441
                     /locus_tag="VF089_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6174094.1"
                     /translation="KAEIQDSMKSAMRSGDKITLESLRLALAAIKNEEIKSRRELTTE
                     EIQRTIGTLCKQRLESIEMFQKGGRSDLVEKEQAELKVLQRFLPEPLSDDEIIAAIRI
                     TIRESGAQGLQDLGRVMKEVMPKVSGRSDGKRVNELAREILRGA"
     gene            631..1176
                     /locus_tag="VF089_08780"
     CDS             631..1176
                     /locus_tag="VF089_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX6174095.1"
                     /translation="MNIVDIILLSLLFLFGLRGYVKGFFREIFSIAGLIVGFMAAARY
                     NHTVATLTEEYWKISPLILKGASFVAIFFAIYLCFKLLGWFIHHSGKFLFLQTLNRAG
                     GIVIGIGKGAAVMALIVFFMSSASWIPASASEKIKTSVLVPSFSRLAEVIIRVGKERI
                     LTPEDNRVRLLSQTCVLEVAA"
     gene            1173..2975
                     /gene="dnaG"
                     /locus_tag="VF089_08785"
     CDS             1173..2975
                     /gene="dnaG"
                     /locus_tag="VF089_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX6174096.1"
                     /translation="MITQEKIAEIRARASVVEVISDYVTLKKVGRNHVGLCPFHGEKT
                     PSFTVNDEKGIFHCFSCKTGGSVFNFLMQYDHLSFPEAVESVGKRYGIAVERVTKNGN
                     SGADAEREALYRLNECAAANYHRVLFNDIVARRALEYLDKRGLEESIARRFMLGFAPP
                     YGSGLLEIIKKEKLSLKDALRLGLIGQREGNRFHEKFFDRLIFPIVNAGGKVIGFGGR
                     VLEQGTPKYLNSSDTPLFRKGSTLYGLYQAKEAIRKSDRAVVVEGYLDVIALHQYGVD
                     YAVATLGTALTVEHVRALSRYTKNVVAFFDGDHAGSRAAERSFEIFIEAGLLGRAAFL
                     PNGEDPDTFIRSRGKEAVEELLNRSVPLADYYFSSLEKRYGKSLEGKSRIAQEISRVL
                     QKVRNPFEVDLLIARAVDSLGIREEYFRKPPQNSVAPKAPLLANPRFSSSDLGNRANA
                     AERSLISLILRYPRIAGYVFGETDVRQWVGSAWQEIIDLIRADWQEHGNVDVARIVQV
                     FDSDVAAQIAGFAIEGENVPEAESQKMADDCIAHLRRKHMREQERNLRIAIRTAEEQK
                     DENATRERILEWQEIVRKERQLERRKLAPKTTAR"
     gene            3032..>4254
                     /locus_tag="VF089_08790"
     CDS             3032..>4254
                     /locus_tag="VF089_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6174097.1"
                     /translation="MKEVKKLIDLGKEKGYLTYDDVNDMLPAEVVSPDQIDDVMSIFG
                     EMDIEVVDSNQRVTLGGAGEDLADDEEDEKEIEGETDGDAVGKTGDPVRMYLREMGTV
                     SLLSREGEVEIAKKIEVGEKKVIEEVLSSPLALRYVLELGEKLAQHEIRVREIIKDGD
                     EDAEYLEDEEVHEKRLLDQIAKIRRAANERDKIKNELEGKRLSAQRRQALQQTLEKYQ
                     ERIVGGLKSLQLNRKQTEAIVDGLKRAMDRMQTLERRVKEFEQRTGKSASELLKLVAN
                     LNGRKKDWQRLTSSLRMGHDAVLHMSEEIKNARRDIRHIEKDLDIPAEEIKRRVWSIV
                     DGETKANLAKKELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYQRG
                     YKFSTYATWWIRQAIT"
BASE COUNT         1137 a          981 c         1181 g          955 t
ORIGIN      
        1 aaggccgaaa ttcaggattc tatgaagtcg gccatgagaa gcggcgataa gatcacgctc
       61 gaaagtttaa ggctggcatt ggcggctata aagaatgagg agatcaaatc tcggagagag
      121 ctcacaacgg aggagatcca acggacgatt gggacgctat gcaaacagcg gctggagtcc
      181 attgagatgt ttcaaaaggg tggccgcagc gatctcgtgg aaaaagaaca ggccgagctg
      241 aaagttcttc aacgtttcct acctgaacct ctgagtgacg acgagataat agcagcgatt
      301 cgaatcacga tccgggagtc gggcgcacaa ggcttgcaag atcttggcag agtaatgaaa
      361 gaagtgatgc caaaggtaag cggccgaagc gatggcaaaa gagtcaatga gctggcccga
      421 gaaatacttc gtggcgcctg aatgcttata gggcacgttc tctttacctt cccatcaaac
      481 gatcaccggc cgctcctttg ctgcctagcg gccatgagcg gcttttaacc aaagaatgtg
      541 acccctgctc gtaatgcatc ccgcaaccat gcttcacgag tgctggtatc taacactaac
      601 gaaagttaac aatacggatg gctgtcatcg gtgaacatcg tcgatatcat cctgctgtct
      661 cttctttttc tttttgggtt gcgaggttat gtcaaaggtt tttttcggga aatattctca
      721 atcgccggcc tgatagttgg cttcatggca gctgcgcggt ataaccacac tgtagcaacg
      781 ctcaccgaag agtactggaa aatctctcct ttaatcctca agggggcttc gtttgtcgcg
      841 atttttttcg ccatctatct ttgttttaag ttgttgggct ggtttattca tcactccggg
      901 aagtttcttt tcctgcagac attgaataga gctggcggta ttgttattgg aatcggtaag
      961 ggtgcggccg taatggcgtt gatcgttttt tttatgagct ccgcgtcttg gattccggct
     1021 tccgcaagtg aaaaaattaa aacttctgtt ttggtcccat cattctcacg tttagccgaa
     1081 gttatcattc gagtcggcaa agaaaggatc ttgacgccgg aggacaatcg ggtgcgactg
     1141 ttaagtcaaa cttgtgtcct agaagttgcc gcatgatcac tcaggagaag atcgctgaga
     1201 ttcgtgctcg ggcctcggtc gtcgaggtca tttccgatta cgtgacacta aagaaggttg
     1261 gccgtaatca tgtcgggctg tgccccttcc acggagagaa aaccccctct ttcacggtga
     1321 acgatgaaaa gggaattttt cactgtttta gctgcaagac cggcggcagc gtcttcaact
     1381 tcttaatgca gtatgaccat ctatcgtttc ctgaagcggt tgagtccgtc ggtaagcgct
     1441 acggcattgc ggtggagcgc gttacaaaga acggcaactc gggagcagat gcggagcgcg
     1501 aggccctcta tcgactcaat gaatgtgccg cagcgaatta tcatagagtt ctgttcaacg
     1561 acatcgtggc aagaagagct ctcgagtatc tagacaaacg tggcctagag gagtcgatcg
     1621 cgcggcgctt tatgttaggc ttcgcaccac cctacggttc agggcttctg gagatcatca
     1681 aaaaggaaaa gctttctctc aaggatgcgc ttcgactggg gctgattgga caaagggaag
     1741 gtaaccggtt tcacgaaaaa tttttcgacc gactcatctt tcccatcgtc aacgccggag
     1801 gcaaagtcat cggcttcggc ggacgcgtgc tggaacaagg aactccgaaa tacctcaact
     1861 caagcgatac cccacttttt cggaaaggat ccaccttgta tggcctctac caagccaaag
     1921 aagcgattcg caagagtgac cgagccgtag tcgtagaggg ttacctcgac gttatagcgc
     1981 tgcaccaata tggtgtggat tatgctgtcg cgactctcgg aacagcgctc actgtcgagc
     2041 atgtacgcgc tttgtcgcgc tacaccaaaa atgttgtggc gttctttgat ggagaccatg
     2101 ccggcagccg agctgctgag cgaagcttcg aaatttttat tgaagccgga ctgcttgggc
     2161 gggccgcttt tttgcctaat ggtgaagatc cagatacttt tatccgcagt cgcggcaaag
     2221 aggcagtgga agaactcctt aatagatcag tgccgttggc agactactac ttctcctcgc
     2281 tagaaaagcg ttacggtaaa agtttggaag gcaaaagccg gattgcccag gagatctcgc
     2341 gcgtgctgca aaaggtgcgt aacccgttcg aagtggatct tctaattgcg cgggccgtag
     2401 attccttagg catccgggag gagtactttc gcaagcctcc gcagaattcg gtcgctccca
     2461 aagcgccttt gttggcaaac ccacggtttt cttctagtga tctcggcaac cgcgctaatg
     2521 ctgcggaaag atccctgata tctctgattt tacgttaccc ccgaatcgcc ggatatgtat
     2581 tcggagagac agacgtgcga caatgggtcg gatcagcctg gcaggagatt atcgatctga
     2641 tcagggccga ctggcaagag catggaaacg tcgatgttgc caggatcgtg caggtcttcg
     2701 attcagatgt cgctgctcag attgccgggt ttgcaatcga aggagaaaac gttccagaag
     2761 ctgagtccca gaagatggcc gatgactgca tagcccacct gcgacgaaag catatgcgag
     2821 aacaagaaag aaatttgcga atcgcgatcc gaactgcaga agaacagaag gacgagaacg
     2881 caaccaggga gaggatacta gaatggcaag aaatcgtacg aaaagaacgc cagctagaac
     2941 gccgaaagct cgcgccgaag acaaccgctc gataggcaac gataaagtcg cctcacatca
     3001 tgctgccggg caaccggcgg agagaaagaa catgaaggag gtgaagaagc tcatcgatct
     3061 cggcaaagag aagggttacc tgacgtatga tgatgtcaac gatatgcttc ctgcggaagt
     3121 cgtttctccg gatcaaatcg acgatgtgat gtctatcttt ggcgagatgg acatcgaggt
     3181 tgttgattcc aatcaacgag tcactcttgg gggggccggt gaagatcttg ccgatgacga
     3241 agaggacgag aaggaaattg agggcgaaac tgacggggat gcggttggca agactgggga
     3301 tccggtccgt atgtacctaa gggagatggg aactgtttca ctgctgagcc gggaaggcga
     3361 agtcgagatc gcaaagaaaa tcgaggtagg tgaaaagaaa gttatcgagg aagtgctgtc
     3421 cagcccgctt gcattgcgtt acgttttgga actcggcgaa aaactagcgc aacatgagat
     3481 ccgcgtgcgt gaaatcatca aagacgggga tgaggatgcc gaatacttgg aagatgaaga
     3541 ggtgcacgaa aaacgcttgc tcgatcagat cgctaaaatc cgcagagccg ctaatgagcg
     3601 cgataagatc aaaaatgaac tcgagggcaa gcgtctatcg gcgcaacgtc gtcaggcgct
     3661 gcaacaaact ttagagaagt atcaggaacg aattgtcggc ggtctgaaaa gcctccagct
     3721 gaatcgcaag caaaccgaag cgattgttga tggtctcaaa agagccatgg atcgaatgca
     3781 aacgctagag cgtcgggtga aggaattcga gcaaaggacc ggtaagagcg catccgaact
     3841 cctgaagctt gttgcaaacc tcaacggacg taagaaggat tggcaacgcc tcaccagttc
     3901 gctacgaatg gggcacgacg ccgtgctgca catgtcagaa gagatcaaga acgcgcggcg
     3961 cgacatacgg catatcgaga aagacctcga catcccggcc gaagaaatca agcgtcgtgt
     4021 ttggtcgatc gttgatggtg agaccaaagc caatctggcg aagaaagaac tgatcgaagc
     4081 aaatctgcga ctggtcgtga gtattgcgaa gaagtacacc aatcgcgggc tgcagtttct
     4141 cgatcttatc caagagggaa atatcggctt aatgaaagcc gtcgataagt ttgaatatca
     4201 gcgagggtat aagttttcca cgtatgccac ctggtggatc cggcaagcaa tcac
//
LOCUS       DASUTG010000382         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1098114, whole genome shotgun sequence.
ACCESSION   DASUTG010000382 DASUTG010000000
VERSION     DASUTG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098114"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(492..740)
                     /locus_tag="VF089_08795"
     CDS             complement(492..740)
                     /locus_tag="VF089_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6174098.1"
                     /translation="MIDLKYSLVIEATDDPTFFGFYSPDLEGFTGVGHSIEDCVYQAR
                     WGMEEHLELLKKQSLPVPTENPNPKIVIQNQEPLAQAS"
     gene            complement(755..940)
                     /locus_tag="VF089_08800"
     CDS             complement(755..940)
                     /locus_tag="VF089_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX6174099.1"
                     /translation="MRFNELVRLLEQNGFRLIREKGSVRYAKAGVDRLIRVDYHGAKE
                     IPTGTCNAILKAAGLKR"
     gene            complement(1317..1607)
                     /locus_tag="VF089_08805"
     CDS             complement(1317..1607)
                     /locus_tag="VF089_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174100.1"
                     /translation="MGKLLAIDPAVIRRLRELPKNERVECLLALCDLVDGFGQPHVHS
                     GLGIRKLGGKLFECRGNRDLRFIFQDRGAELFVSFLGNHDEIRALLRRGSYR"
     gene            complement(1600..1878)
                     /locus_tag="VF089_08810"
     CDS             complement(1600..1878)
                     /locus_tag="VF089_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174101.1"
                     /translation="MKTLTITDAKKNLGRWLAAAARGEDIGIVCGSDIVALRRVEVES
                     TDYAQREYGALPQQVAALEKATEQRYRRLKRGKKLTSITAGELRKMFG"
     gene            2209..3327
                     /locus_tag="VF089_08815"
     CDS             2209..3327
                     /locus_tag="VF089_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174102.1"
                     /translation="MAIIDLDSHFRDGWLLDEIYQLPEPFARHSPRRIGEGKYFYSKF
                     EHDLSPMDDPVANANFKKPVTHQVFYNPEANQRGNEVMRWQQGGYDMEYRLKDNGREN
                     IDFQLIFPTGIDIPSQNAGALGAAVARAYNDWAHKLVAGYRDRLSPVAMMPAGCPEEM
                     ASELRRCVKDLDCKAAHLVSYTKERQMDDPAFFPFYDAAQELDIPLFCHPSTLGDVGT
                     LINRQSHFFPMHILGRPLNCVAPLVAMVLGGIFEKYPRLKIVFFEVTAEFLVFWMHRM
                     DDDYEVIKDAGMCPQLSMLPSEYVRRNCYVTCEADEKCLPLALAEVGDSHVLMATDYP
                     HFDSTFPKTVSGIRERPDISPKQKKLILEDNALNLIPM"
     gene            3421..4425
                     /locus_tag="VF089_08820"
     CDS             3421..4425
                     /locus_tag="VF089_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174103.1"
                     /translation="MIRQSIFALFFSLSISFSAAAQDAKLIEQAKKEGGKLIVYGSLE
                     SFTVEPIAQAFQKKTGIAVEYWRASATKVMDRALSETRAGKALFDVMLNNSGAMHVLK
                     KEGLFIPYSSPATAAFPKENTDPQLGPIYRNTPIGIVYNTGAIKPADAPKSLEDLLNP
                     KYKGKFVMPDPTQHTTTLQWIASLYKIMGKEKAEKFTRDLGATKPLLVESLAPAAERV
                     STAETPIGISLVRYVVTFSEKGAPVDYVRLGKMLSLGQYIALSSKASRPNSGKAFIDF
                     FLGDESMRMLAKQGEFVNRKGIYPSLPDADKIQAVEMDDFDAQGFKEKTQEYQKIFLK
                     "
     gene            4707..>4930
                     /locus_tag="VF089_08825"
     CDS             4707..>4930
                     /locus_tag="VF089_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174104.1"
                     /translation="MNAPLKVSGIDHVVFHVKDLARARKFYVDFLGLEVDHERDWQCF
                     LKCGKQGIALFEVDEGEEVHGGSEVNHMALR"
BASE COUNT         1225 a         1344 c         1248 g         1113 t
ORIGIN      
        1 ctctccttga agggctcctg atagccttcg gatattggaa gagggagggg gaaccgcgga
       61 ataccttcgc tagtcaaacc ggagagcgtg gcccagtctt cttacctctt gcgcgccatt
      121 acagctttcg aaaatccatt acacagctgc atttctagtt aaaggtaacc atcgtctgcc
      181 tgagaagcaa atgcgtcctg tctaatagtg aacaagtttg aaaagaatta ttcgcgataa
      241 gatcatgggg atcggtccgt tccagtaatt ccaccgcttc gtctacgtta aaaactcgca
      301 ccaacagccg ttcaacggtt caatagttca acagttcaaa ggttcaatcg ttcaacgacg
      361 aagaggaact tccacgtttc gagaattccc gaacgtcgaa atgaagcagc ggcaaagaaa
      421 aatgcgaaga atactgggac ggtttggaaa ccggaacgtc gaagtagata ggtaaagcta
      481 cggaggaaac tctaactcgc ttgtgctaat ggctcttggt tttgaataac gatcttcgga
      541 ttcgggtttt cggtaggaac cggtagcgat tgttttttta acagctcgag atgttcctcc
      601 ataccccacc tggcttgata aacgcagtct tcaatagagt gaccgacgcc ggtaaagcct
      661 tcgagatccg gcgagtaaaa cccgaaaaaa gttggatcat ccgtcgcttc gattaccaac
      721 gaatatttta gatcaatcac ggcacttctc ctaactatcg cttcaaaccg gcagccttca
      781 gaatggcgtt gcaagttccc gtcgggattt ccttggctcc atgatagtca acccgaatca
      841 gtctgtccac accggctttg gcatagcgaa ccgaaccttt ttctcggatg agtcgaaaac
      901 cattttgctc taacagccgt acaagctcat taaatctcaa cgtctcctcc aacccgatgc
      961 ggatagtaac aaagcatgga tgagaaagga aagacacaca agcggtcagc tttccaagca
     1021 aggctgttca gacgtagcaa tgtcagatcg caacggattt aagattcgaa agttcaatgg
     1081 ttcaacgaga gacgtgaact tccacgtttc gagaattccc gaacgtcgaa atgatagatc
     1141 aatccaatcg gctggaagcg tgaacgagcc ccatcggatc gagccgttcc atcggttcca
     1201 gtagttccat gactaccctc tgttccagtg gttaacaatc tcaaaatctg tgagagattt
     1261 tgagcgctca atgttctagc tctgcggcag gatctcagcg atttccgact aagtgatcaa
     1321 cgatagctgc ctcggcgaag gagggctctg atctcgtcat ggttgccgag aaaggaaaca
     1381 aaaagctccg cgcctcggtc ttgaaagata aatcgcagat cgcggttgcc gcggcattcg
     1441 aagagcttgc cccctaattt gcgaatcccc agcccgctgt gcacatgagg ttggccaaaa
     1501 ccgtccacca gatcgcaaag agcaagcagg cactcaactc tttcgttctt tggcaattcg
     1561 cgcaggcggc ggatgaccgc gggatctatg gcaagaagct tacccaaaca tcttcctaag
     1621 ctctccagcc gttatcgacg tgagcttttt gccccgtttc aaccgccgat aacgctgctc
     1681 ggtggctttt tccaaggcgg ccacctgctg gggcagagcg ccatactccc gctgcgcgta
     1741 atcggtggac tcgacctcga ccctcctgag cgcaacgata tcggaaccgc aaacaatgcc
     1801 gatatcttcg ccccgcgctg cagcggcaag ccagcgtccg agattcttct tcgcatcggt
     1861 aatcgttaaa gtcttcataa agtctttata aagccgataa aagaacttgt caacaatgcg
     1921 aaatcatggt tccaaatgca acggcagttc aacagttcaa agcgagacag ttagcgagcg
     1981 gggcgcagcc ataaaccttg ctgacttcta attgttcgaa tcgtcctcgg acgttccatc
     2041 gcttcgctct tccgcggatc ggagccgttc caatcgttcc agtagtttca gcgcttcgct
     2101 ccgttcttat tgacgggcga gcggagctgt actagcttcg gagcgtagcg cattctggcc
     2161 ggccacctgc ttgccggtcg cacattgatt gcgcaaaaag gagctctcat ggccattatc
     2221 gatttagatt cccactttcg cgacggctgg ttactcgacg agatttacca actacccgaa
     2281 ccgtttgccc gccactcccc gagacgcatc ggcgagggca aatatttcta ttccaagttc
     2341 gagcacgatc tctctcctat ggacgacccc gtagccaacg cgaatttcaa aaaaccggtg
     2401 acccaccagg ttttctataa cccggaagca aaccagcgcg gcaatgaggt catgcgctgg
     2461 cagcagggcg gctatgatat ggaatatcgc ctgaaggaca atggccgcga gaacatcgac
     2521 tttcaattga tcttccctac cggcatcgac attccgtcgc agaatgccgg cgcgctcggc
     2581 gccgccgtag cgcgagccta caacgattgg gcacataagc tcgtcgccgg ctatcgcgat
     2641 cggctctcgc ccgtcgccat gatgcccgcc gggtgtccgg aagaaatggc cagcgagtta
     2701 cgccgttgcg tcaaagattt ggactgcaaa gccgcccacc ttgtaagtta caccaaagag
     2761 cggcagatgg atgatccggc ttttttcccg ttctacgacg cggcgcaaga actcgatatc
     2821 ccgcttttct gccatcccag tacgctcggc gatgtcggca cactgatcaa tcgtcagagt
     2881 catttctttc caatgcatat tcttggccgg ccgctcaact gcgttgcgcc gctcgttgcc
     2941 atggttctcg gcgggatttt cgaaaagtat ccgaggttaa aaatcgtctt cttcgaagtg
     3001 accgccgaat tcctggtgtt ctggatgcac cgcatggatg acgattacga agtcatcaag
     3061 gatgccggca tgtgcccgca gctgagcatg ttgccgtcgg aatacgtcag gcgaaactgt
     3121 tacgttacct gtgaagccga tgagaaatgc ctgcccttgg cgttggccga ggtcggcgac
     3181 agccacgttc tgatggcgac ggactatccc cacttcgatt cgacgttccc caaaacggta
     3241 agcggtattc gcgagcggcc ggatatttca ccgaaacaaa agaaactgat tctcgaagat
     3301 aacgcgctca atctgatccc tatgtaggtc gaggaaacga tccaaacccc agaccatgaa
     3361 cgcttacgca catggctcga gcttcggatt cgtagtgaac ttatcatggg agagcttgtg
     3421 atgattcgtc aatcgatttt cgctttgttc tttagcctgt cgatttcgtt tagcgccgca
     3481 gcacaagacg ccaagctcat cgagcaggcc aaaaaggaag gcggtaaact gatcgtctac
     3541 ggctcgttgg agtcatttac cgtcgagccc attgcccaag cttttcaaaa gaagaccggc
     3601 atcgcggtgg aatactggcg cgcttcagcg accaaggtga tggatcgggc gctgagcgaa
     3661 acgcgcgccg gcaaggcgct ctttgacgtg atgttgaaca acagcggcgc gatgcacgtg
     3721 ctcaaaaaag aaggtttgtt tatcccgtat agttctccgg caaccgcggc cttccctaaa
     3781 gaaaacaccg atccgcaact tgggccgatc taccgcaata ctccgatcgg catcgtctac
     3841 aataccggcg cgatcaaacc cgccgatgcg cccaagtccc tcgaggatct gctcaatcct
     3901 aaatacaagg gcaaattcgt catgcccgac ccgacgcaac acacgactac cctgcagtgg
     3961 atcgccagcc tgtataaaat aatgggtaaa gagaaggcgg aaaagttcac gcgcgatctc
     4021 ggcgccacca agccgcttct ggtggagtct ctggcgcccg cggctgaacg ggtatccacg
     4081 gccgagactc ccatcggcat cagcttggta agatacgtcg tgacgttttc ggagaagggc
     4141 gcgccagtcg actacgtcag gctcggcaaa atgctcagcc tggggcaata catcgcgctt
     4201 agcagcaagg cgtcacgacc taacagcggc aaggcattca tcgatttttt tctcggcgat
     4261 gagagtatgc gcatgctcgc caaacaaggg gaatttgtaa atcgcaaagg catctatcct
     4321 tcgctgcccg acgcggacaa gattcaagcc gtggaaatgg atgacttcga tgctcaggga
     4381 ttcaaggaaa aaacccagga gtaccagaag atttttctga aatagtaaaa ggggctagcc
     4441 cggattattc atgttcagag caaaaactac gacactatgt cgatcgaagg gtgagcctga
     4501 aggagtgaag acatctagac caacagcttc aatgaagcct ttggtgcttg attccctgct
     4561 cgcacttttc ccaggcgtcc gctccacaac tgtgttatgc aggccgctcc aaggcacagg
     4621 cccatttgtc ttcgttccgg agggctcgcc ttcgatcacc gtcaccagtg aataatccgg
     4681 gctagattct tttagggagg ttgatcatga acgcacctct caaggtaagc ggtatcgacc
     4741 atgtggtctt tcacgtcaaa gatctcgcgc gcgcccggaa attttacgtc gatttcctcg
     4801 gactggaagt cgaccacgag cgcgattggc agtgcttcct caaatgcggc aaacagggca
     4861 tcgcactctt tgaagtcgat gagggcgaag aagtgcacgg cggcagcgag gtcaatcata
     4921 tggcgctgcg
//
LOCUS       DASUTG010000383         5938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1691620, whole genome shotgun sequence.
ACCESSION   DASUTG010000383 DASUTG010000000
VERSION     DASUTG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691620"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF089_08830"
     CDS             <1..339
                     /locus_tag="VF089_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6174105.1"
                     /translation="TGANLLVNLTNDAWYGKSVGPWQHARLAQSRAIETRRSLLRVTN
                     TGVTSVVNAKGELVESLPMFVPDVLRAQVDILDGQTYYVRYGDWFAWGMTITTAAILL
                     FGLNRASMVR"
     gene            complement(465..1748)
                     /locus_tag="VF089_08835"
     CDS             complement(465..1748)
                     /locus_tag="VF089_08835"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX6174106.1"
                     /translation="METRWIRSMVFIFVLGSFLSSAGADSDDKRQFPTSATFTTLITT
                     PRPIEGLTGDSHRNLYTGGSGMPPCPIWQINLNNPALTPVGFVSPVTGNCGFSGIALN
                     EIGDLFIADGGAGTIYTFTPNADTPPTAIVFASGAPGANGLAFDKKGNLWASDGTTGQ
                     GRVWKITGSGANCASAAAINCQEVFRIQPMANEVNLVNGVGGVGRDNRALPLGTITVT
                     PTSRNAANTAGSQPLVANGLAFNKKGDLFNIDTARGALWKVEFDQDGNLESPTGCDST
                     FTANTLCLSNVFVAHPILEGGDGIALDRQGNVWVAANERNAVAVVTKDQKVIEVFRNP
                     VNLPQPISFGAGLRNAGDQSVGNNHILEFPTSPFIIGKVLCVSQSDGDRRDNSPRSAG
                     EVNAGAPSDNVNRLGKISCMDQELKIPGLPLPIDD"
     gene            complement(1995..2663)
                     /locus_tag="VF089_08840"
     CDS             complement(1995..2663)
                     /locus_tag="VF089_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6174107.1"
                     /translation="MKIFMTLLALLAVPFSAAAQDALKRDPHEMHQLHRDSNAYISML
                     EDPKRDAYQKPHEVLTALAIKPGEVIADIGAGSGYFTFRLAHHVGDKGKVYAVDVSPD
                     MILHLNRRIRELKAMNVSSILADPDDPLLPEMWVNRFFFTNSWHHIENQAKYLSLIKK
                     ILKPGGEVIMIDFHKKELPVGPPMQMKIAREDVIKEMEANGFRLAKEHTFLPYQYFLM
                     FVAK"
     gene            2855..4246
                     /locus_tag="VF089_08845"
     CDS             2855..4246
                     /locus_tag="VF089_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6174108.1"
                     /translation="MAQQELKRQIGLFSAVMLIAGDMIGTGIFISTGVIAETLPSPGG
                     VLLVWLLGGLLALTGALTFAELSASLPFAGGDYIYIREAYGKLMGFLSGWSSFLVTFS
                     GAIAFLAVSLNGFMAFFFPVLRSETILFSLALPWLPITATAGTIFAIVVVLILSALHC
                     LGVRQGTMVQNILTILKIGALLGIIVFGILFGKGDTAHFVPLFDWQKVSFSVFAAAFI
                     PVIFAYSGWNAITYIAGEVKDPDRNLPRALLFGVLIVTGLYLAINAVYIYAVPVTEMK
                     GALRVSEVATTALFGYQTSALITAIITISILGALNVVTMIGPRIYYAMAQDGVLFRSL
                     SRIHPRFGTPSSAIILQAIWACLLILTNTWGTLFTYVSVVITLFSAFTVGSVIVLRYK
                     RPELKRPYKVWGYPLVPLLFVVIHLWIVWGSVKEKPFESLVGVVIVAIGIPIYFIWKT
                     LGPAQKPSFASRS"
     gene            4294..4902
                     /locus_tag="VF089_08850"
     CDS             4294..4902
                     /locus_tag="VF089_08850"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6174109.1"
                     /translation="MAIRITRVYTRTGDRGETSLVGGKRVPKDSPRIDAYGTIDELNS
                     VIGLARVFNEENLDAGEAHHFLDEVLCKIQDELFDLGSELATPPEFFQEGMFRVSQAE
                     VKNIEKLIDRCQQNLQPLESFILPGGGRVGAYLHQCRTVCRRAEREILRLSREETISE
                     WPLKYVNRLSDLFFVLSRWISKETGEREYLWQRGLQTKAKGR"
     gene            5039..5809
                     /locus_tag="VF089_08855"
     CDS             5039..5809
                     /locus_tag="VF089_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX6174110.1"
                     /translation="MGLLVGKRAAIFGVANDRSIAWAISEALHAEGAELAFTFAGEVL
                     EKRVRPLAEGIGSKIILPCDVTKDEEIEKVFSTLKQEWGALDIMIHAIAFARKEDLAN
                     PYVQTSRHGFHLAMDVSAYSMVALARHAAPLMEGGGGSMLTLTYMGSERVIPNYNVMG
                     VAKAALEASVRYLAHDLGPKGIRVNAISAGPIRTLAASGIADFKEMLHHVGERAPLKR
                     NIDAAEVGKTALFLCSEWGSAITGEVLHVDAGYSIMGK"
BASE COUNT         1318 a         1488 c         1661 g         1471 t
ORIGIN      
        1 accggcgcca acctcctggt caatctcacc aacgacgctt ggtacggcaa atcggttgga
       61 ccttggcaac atgcgcgctt ggcgcaatcc cgcgccattg aaacccgccg cagcttgctc
      121 cgtgtcacca acaccggggt gacctcagta gtcaatgcca aaggagaact ggttgaatct
      181 ttgccgatgt ttgtgccgga cgtgctaaga gcgcaggtcg atatcttgga cggtcagacc
      241 tactatgttc gttatggcga ctggttcgca tggggcatga cgatcaccac ggcggcgatt
      301 cttcttttcg gtctgaacag agcctcaatg gttagatagc gttagctaaa tctcggttca
      361 gaggaccggc aaacaaaaag caaaacgccc gaggacgaaa acagatgcca gaaagacgtt
      421 ctctatgccg cgttgctggt ctacgtttgc tgtcgtttaa accactagtc atcgatcggc
      481 agcggcaggc cagggatttt cagctcctgg tccatgcagg agatttttcc taatcgattt
      541 acgttatcag agggagcccc cgcattcact tcaccggcgc tccggggaga attgtcgcgc
      601 ctgtcaccat cggattgaga aacgcataag acctttccta ttataaaggg gctggttgga
      661 aattcgagga tgtggttgtt gccgacggac tggtcgcccg cgtttctcaa tccagctcca
      721 aatgatatag gctgaggtag gttaacgggg ttgcgaaaca cctcgatgac cttttggtct
      781 ttggtgacca cagcaactgc attccgttca ttcgcagcca cccagacatt tccctggcgg
      841 tcgagcgcga tgccgtcgcc gccctcgaga atcggatgag cgacgaagac attactaagg
      901 cacagcgtgt tggccgtgaa cgtgctatcg cacccggtcg ggctctcgag atttccgtct
      961 tggtcgaact ccaccttcca aagcgctcct cgagctgtat cgatgttgaa gaggtcgccc
     1021 ttcttgttga aggccaaacc gttggcgacg agcggctggg aacctgctgt gttggcggcg
     1081 ttacgactcg ttggtgtcac ggtaattgtg cctagcggca aggcacggtt atcacgtccg
     1141 actccaccga caccattaac taggttcact tcattggcca tgggctgaat ccgaaatacc
     1201 tcctggcagt taattgccgc tgcggaagcg cagtttgcgc cggagcccgt tattttccaa
     1261 acccttccct gacccgtcgt gccgtcgctg gcccagagat ttcctttctt gtcgaaggca
     1321 agtccattcg caccgggcgc gccggaggca aaaacgatcg cagtcggggg cgtgtccgcg
     1381 tttggtgtaa aagtgtaaat ggtacccgcg cctccgtcgg cgatgaacag gtcgccgatt
     1441 tcattcaaag cgataccaga gaagccgcag tttcccgtga ccggggaaac gaacccgacg
     1501 ggagttaggg cagggttgtt taaattgatc tgccaaatgg ggcaaggagg catccccgag
     1561 ccgcctgtgt ataagttgcg gtgactatcg ccggtcagcc cttcaatcgg gcgcggagtg
     1621 gtgatgagcg tcgtaaaggt ggcactggtt ggaaactgtc gtttgtcgtc gctatctgcc
     1681 ccagccgagg acaagaatga gcccaggaca aagataaaga ccatagatct gatccatctc
     1741 gtttccatga gagtcctcct ttgcttaaat gaccccaagc atttgtcccg gatggtagat
     1801 gagtgtgagc ttattcctca agttatgaga cgtgtcaagc gacttgaatg gataagcatt
     1861 catcgatatg aagctttggt taagccgaaa aatgccgctc tccgatacat aaacagattg
     1921 ccgcaagtga cacgaatcta actaaaaccg ttgcggctat tagctgagaa ttcccatcgc
     1981 cggcgacttc ctgcctactt cgctacaaac attaaaaagt attgataggg caaaaatgtg
     2041 tgctctttcg caagccgaaa cccgttggct tccatttctt tgatcacatc ctcgcgagca
     2101 atcttcattt gcatcggtgg accaacggga agttctttct tgtggaagtc gatcatgatc
     2161 acttcgccgc cgggtttgag tattttttta atcagcgaga ggtacttcgc ctgattctcg
     2221 atgtggtgcc atgaattcgt aaaaaagaaa cggttgaccc acatctcagg cagtaacgga
     2281 tcgtccggat cggccaggat agaactgacg ttcatcgctt tgagctcccg gatgcgccga
     2341 ttgagatgca gaatcatgtc cgggctgacg tcgacggcat acacctttcc tttatcgccg
     2401 acgtgatgtg ccaggcgaaa ggtgaaataa ccggagcctg cgccgatatc ggcgatcact
     2461 tcacctggct taatggccag cgcagtcagc acctcgtgcg gcttttgata ggcgtcgcgt
     2521 ttcggatctt cgagcatgct gatgtaggca ttcgaatcgc ggtgaagttg atgcatctcg
     2581 tgaggatcgc gcttgagtgc atcctgtgcc gctgctgaga acggcactgc cagcaatgcc
     2641 agaagagtca taaatatttt cattctcacc tcgttgaatg cgctcggagt tgacgagagc
     2701 ttaggttcac ggctacgcga gcgaagattg tcatgaccgc gcgctccatc gatcgtgtcg
     2761 gctgaacaat ggagtgaagc tagcacattt tcaacgacct ccaagggcta aatcagttgc
     2821 gagcttggct gcttccctgc tatgggtatt cgctatggcg caacaagagt tgaagcgaca
     2881 aattggtttg ttcagcgcgg tgatgttgat tgccggcgat atgatcggca ccgggatttt
     2941 catttcaact ggggttatcg ctgagacatt gccatcgccc ggcggcgtgt tgctggtgtg
     3001 gttgctcggc gggctgctag ctctgacggg ggcgcttact ttcgctgagc tttcggcgag
     3061 tttgccgttc gccggcggcg actacatcta catccgcgag gcctatggta aattgatggg
     3121 gtttctctcc gggtggtcct cgttcttggt aacgttctca ggtgcgattg catttcttgc
     3181 cgtgagctta aatggcttta tggctttctt ttttccggtg ctgcgttccg aaaccatttt
     3241 attctcattg gccttacctt ggttgccgat cactgccaca gcagggacga tattcgcgat
     3301 agttgtggtc ctgattttgt cggcactgca ttgtctgggg gttcgtcaag gcacgatggt
     3361 gcaaaacatt ctgacgattc taaaaatcgg cgcgttgttg ggaatcattg tcttcggcat
     3421 cctcttcggc aaaggcgata cggcgcattt tgttccactt ttcgattggc aaaaagttag
     3481 cttcagcgtc ttcgctgctg cctttatacc ggtaattttt gcctattcgg gctggaacgc
     3541 gattacctat attgcaggcg aggtgaaaga tccggacaga aatttgccgc gcgctttact
     3601 attcggggtg ctgattgtta ccgggctcta cttggcaatt aatgcggtgt acatctacgc
     3661 tgtgccggtt accgagatga aaggcgccct gcgcgtatcg gaagtggcga ccactgcatt
     3721 attcggttac cagacctcgg ctttaatcac cgcgatcatc acgatatcca ttctcggcgc
     3781 gctcaacgtc gtgacgatga tcggtccgcg aatttactat gcaatggcac aggacggagt
     3841 tctgtttagg agcctgagcc gcattcaccc gcggtttggc accccgagca gcgccattat
     3901 cctccaggca atttgggctt gtttgttaat actgaccaac acctggggca ccttatttac
     3961 ctatgtgagc gtggtcatca ctttattctc agcgttcacg gttggctctg tgatcgtgct
     4021 tcgctacaaa agaccggagc tcaagcgtcc ttacaaggtc tggggctatc cgctcgtgcc
     4081 gcttctgttt gtcgtgatac atctatggat cgtctggggc tcggtgaaag aaaaaccgtt
     4141 cgagtcgctc gtgggggtcg tcatcgtcgc catcggcatt ccgatctatt ttatttggaa
     4201 gaccctcggt ccggcgcaaa agccgagttt cgcttcgaga tcttaaatag aaatctcaag
     4261 acgatagttc tgcaaggacg agaaccatcc gcaatggcaa tccgtatcac cagagtttac
     4321 accaggaccg gcgatagagg agaaaccagc ctcgtcggtg gcaaacgcgt gcctaaggat
     4381 tcgccgcgta tcgatgctta cggcacgatt gatgaattga attccgttat cggactggcg
     4441 cgcgtgttca atgaggaaaa cctcgacgcg ggcgaagcgc accatttctt ggatgaggtg
     4501 ctctgcaaaa tccaggatga gctgttcgat cttggcagcg aactagccac gccgcctgaa
     4561 ttctttcagg aaggcatgtt tcgcgtttcc caagctgaag ttaagaacat cgaaaagctc
     4621 atcgacaggt gccagcaaaa tctccagccg ctcgaatctt tcattcttcc tggcggtggg
     4681 cgtgttggcg cttatctgca tcaatgccgg accgtatgcc ggcgcgcgga gcgagagatt
     4741 ctgcggttgt cgcgggagga gacaatcagc gaatggccgc tgaaatacgt caaccgcttg
     4801 agcgatctgt tctttgttct gtcgcgctgg atcagcaagg agaccgggga gagagaatat
     4861 ctatggcaga ggggacttca aaccaaggca aaaggcagat aattctagaa ttggattggc
     4921 gatttagatt gggctaacgg aaaaacggga tttatgaaag tcttggttcc gagatctagt
     4981 ttcaccattc aaaatctcaa acccaattta acatttaaaa tgggtcgggg gggatcatat
     5041 gggcttactg gtggggaaac gggcggcaat cttcggagtg gctaatgacc ggagtatcgc
     5101 gtgggcgatt tcggaagcgc tgcatgccga aggcgcggag ctggccttta ctttcgccgg
     5161 cgaagtcctg gagaagcgag tgcggccgct ggcggagggc atcggttcca agatcatttt
     5221 gccgtgcgac gtgaccaaag acgaagagat cgagaaggtt ttctcgaccc taaaacaaga
     5281 gtggggcgcg ctggatatta tgatccatgc catcgccttc gccagaaaag aagatttagc
     5341 caatccgtat gttcaaacca gccggcatgg gtttcacctg gcaatggatg tgagcgcgta
     5401 ctccatggtc gcgctggcac gccacgcggc gccgcttatg gagggagggg gcgggtcgat
     5461 gctgaccttg acctacatgg gttctgagcg agtcattccc aattataatg taatgggagt
     5521 cgcaaaggcg gcgctcgagg cgagcgtgag atatctagct catgatctcg ggcctaaagg
     5581 gattcgggtc aacgcgattt ccgctggacc gattagaact ctggcggctt caggtatcgc
     5641 cgattttaaa gaaatgctcc atcatgtcgg tgagcgagcg cctttgaaga gaaacatcga
     5701 tgccgccgaa gtcggcaaaa cagcgctctt tctctgcagc gagtggggga gtgctatcac
     5761 cggtgaagtg ctgcatgtag atgccggcta cagtatcatg gggaagtagt atctaaattg
     5821 agcgaacgga gtatcggtaa ggaactgtct gctgagactc gcttacgttc tccagtttcc
     5881 agtattgcag tcgtccagca ctctggagag tgcttcatgg cgatcaaaaa agttacgc
//
LOCUS       DASUTG010000384         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1841222, whole genome shotgun sequence.
ACCESSION   DASUTG010000384 DASUTG010000000
VERSION     DASUTG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841222"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF089_08860"
     CDS             <1..365
                     /locus_tag="VF089_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174111.1"
                     /translation="QLLVSKNCLVEVLSSAALAAEVIARVSEGNPALVCIATVAPDEL
                     VQVRYLSKRLHASFPDLKIVIGRWGLRELDEDGGSPTDDSGEMGSTLLQTRDQITNLR
                     QLISDVDAQNRSEMLPAN"
     gene            complement(517..1908)
                     /gene="asnS"
                     /locus_tag="VF089_08865"
     CDS             complement(517..1908)
                     /gene="asnS"
                     /locus_tag="VF089_08865"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX6174112.1"
                     /translation="MDQQSVSVKQAFTLPVGASITLRGWVRSRRDSKGITFIEINDGS
                     RFRSMQAVVDSGVVTDEILKQVTTGSSIAASGVLVQSPAKGQAVELKVSQIHVYGSAD
                     PATYPLQKKGHTLEFLREISHLRVRSNTFGAAFRVRNALTHAIHTFFQERDFIYVQTP
                     IITTSDCEGAGQMFNVTALNMQQPARTADGKIDWQHDFFGRPAYLTVSGQLEAETFAL
                     AFSRVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYELADNMLLAEEFLKYIIRYVLD
                     QCREDLEFFNERIEKTVLSTLEHVAESSFGHITYTEAIQELKKSGRAWEFPVDWGHDL
                     QTEHERFLSEEVFKKPVIVTDYPKDIKPFYMRVSEDDRTVRAMDVLVPRVGEIIGGSQ
                     REERYDMLLQRLRASGLDEKAYWWYLDLRKFGSAPHSGFGLGLERIMMYLTGLKNIRD
                     VIPFPRTPGNADF"
     gene            1996..>2497
                     /locus_tag="VF089_08870"
     CDS             1996..>2497
                     /locus_tag="VF089_08870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6174113.1"
                     /translation="MKIAQISSVYLSIPPKTHGGTERIVFDLCQHLTRRGHQVELFAS
                     GDSKVDCLLHAVLPVASEDDPQSTFYLEKEFETRNTHNLYRQADRFEIIHAHWPTVAP
                     YFSCFSKTPTLITYAYIEKELHEYYRTNFPRCFPVCISRAQARMLGDESLPVVYNGID
                     LERIPFN"
BASE COUNT          554 a          659 c          688 g          596 t
ORIGIN      
        1 gccagctact ggtttcaaaa aattgtttag tggaggtcct gtcgtcggct gctttggccg
       61 cggaagtaat agcacgggtg agcgaaggca atccagctct ggtttgtatt gcaaccgtcg
      121 cccccgatga gttggttcag gttcgctatc tatcgaagcg cctgcacgcg tccttccccg
      181 atctcaaaat tgtcattggt cgatggggtc tcagggagct tgatgaagat ggagggtcgc
      241 caacggacga ctcaggcgag atgggatcaa ctttgctgca gactcgcgat cagatcacca
      301 acttgcgcca gcttatctcg gatgtcgatg cccaaaaccg cagcgaaatg ttgcccgcca
      361 attaggattt ttcctcgaaa tcttgctctc tcatggatca agcgttcgtt tagagcaact
      421 ctagcctgat agttccagaa cgttaggcgt gttagcaacg tcgctatttt acccttgcaa
      481 taagctgtgc ggcgctttgc aggaccagac ctgcgatcaa aaatccgcat tgcccggcgt
      541 gcgcgggaag gggatgacgt cgcggatgtt tttcagtccg gtgagataca tcatgatgcg
      601 ttcgagtccc aggccgaagc cggagtgagg ggcggagccg aattttcgca gatcgagata
      661 ccaccagtaa gctttctcgt cgagaccgct ggcgcgaagc cgttgcaata gcatgtcgta
      721 acgctcctcg cgctggctgc cgccaatgat ctcgccgacg cgcggaacca gcacatccat
      781 ggcgcgcacg gtgcgatcgt cttcgctgac ccgcatgtag aacggtttga tgtctttggg
      841 ataatccgtc acgatcaccg gctttttaaa gacttcttcc gaaagaaagc gctcgtgttc
      901 ggtttgcaga tcatgacccc agtcaacggg aaattcccag gccctgccgg acttttttaa
      961 ctcctgaatc gcttcggtgt aggtgatatg gccaaagcta gattccgcca cgtgttcgag
     1021 cgtgctcagc acggttttct cgatgcgctc attgaagaac tccaagtctt cgcggcactg
     1081 atcgagaaca tagcggatga tgtattttaa gaattcctcg gccaaaagca tgttgtcggc
     1141 aagctcgtaa aaagccatct caggctcgat catccagaac tccgccagat ggcgtggcgt
     1201 gttgctgttc tcggcgcgaa aagtcgggcc gaaggtgtaa acgcgggaga aggcgagagc
     1261 gaatgtttcc gcttcgagtt gaccgctgac ggtaagatag gcgggacggc caaaaaaatc
     1321 gtgctgccaa tcgattttgc cgtcggcggt acgcgcaggt tgttgcatgt tgagggcagt
     1381 aacgttgaac atctgtcccg cgccttcaca gtcagacgtc gtaatgatcg gtgtctggac
     1441 ataaatgaaa tctctttcct gaaaaaaagt gtgaatcgcg tgagtgagcg cattgcgcac
     1501 gcgaaacgcg gcgccgaagg tgttgctgcg cacccgcaga tgggaaatct cgcggagaaa
     1561 ctccagcgtg tgaccctttt tttgcagtgg ataagtggcg ggatctgcgc tgccgtagac
     1621 atgaatctgt gagactttga gttccaccgc ctggcctttg gccggcgatt ggaccagcac
     1681 gccgctcgct gcgatgctgc tgccggtggt gacttgtttc aggatctcgt cagttactac
     1741 accgctgtcg accaccgctt gcatgcttct gaagcgagaa ccgtcgttga tttcgatgaa
     1801 tgtaatccct ttggaatcgc gccgggaacg cacccaaccg cgcaatgtaa tggaggcgcc
     1861 caccggcaga gtgaaagctt gcttgacgct gaccgattgt tggtccatac accggaagta
     1921 tcgaagttca gcttggaatg tcaaccgcgg agcaagtgct atgagaggcg aaacaatgga
     1981 tggttttcta gcgctgtgaa gatcgctcag atctccagtg tttatctttc aatcccaccg
     2041 aagactcacg gcggaacgga gcggattgtt tttgacctct gccagcacct taccaggcgc
     2101 gggcatcagg tcgagttgtt tgcttccgga gactccaagg tcgattgtct cctgcacgcc
     2161 gtgctgcccg tggcaagcga agacgatcct cagtcgacgt tctatttgga aaaggaattc
     2221 gagacgcgca acacccataa tctctaccgc caggctgatc gcttcgaaat cattcacgcc
     2281 cactggccta cggtagctcc ttatttttct tgcttttcaa agacgcccac cttgattacc
     2341 tacgcataca tcgagaaaga gctccacgaa tattatcgga ctaacttccc gcgctgcttt
     2401 cctgtttgca tcagccgggc gcaagccaga atgctcgggg acgaatcatt gccggtggtc
     2461 tataacggga tcgacctcga gagaattcct tttaaca
//
LOCUS       DASUTG010000385         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1098586, whole genome shotgun sequence.
ACCESSION   DASUTG010000385 DASUTG010000000
VERSION     DASUTG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098586"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF089_08875"
     CDS             <1..363
                     /locus_tag="VF089_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HEX6174114.1"
                     /translation="PYHLKSGTWAEKREQFGDTVIDTISEYAPNLKDIILHRQIVTPW
                     DLEDEFGLTEGNIFQGELTLDQLFFLRPVPGWSRYRTPIKNLYMCGACTHPGGGVMAA
                     SGRLAALEILKDVKRSFI"
     gene            369..1964
                     /locus_tag="VF089_08880"
     CDS             369..1964
                     /locus_tag="VF089_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6174115.1"
                     /translation="MQHYDAIIVGAGHNGLVTAAYLARSGKKVLVLERRSMIGGVAAT
                     EEVFPAFKCSTGAHLAGSFSREISAELELQKHGLKLVSVDPLVFAPQIDGTSLLLASD
                     PAKIGAEIGRFSRADAEKFSSFCSLIDKLARFLRVLNALPLPDGTSPRSLNLSELVKI
                     GVKFHRLGKKEMYEFLRILPMSIADLLGEWFENETLKAAIAAGAIVNSFVGPRAQGTA
                     YVLLHHLSQQANGPFRSAGFISGGINQLPQALARAARQHATEIRTGAEVVRIITKDGA
                     AKGVVLQNGDDFSASVVISNADPKRTLLHLVEPTYLDPHFLLQIKNIRARGTVAKVNF
                     ALDTLPQFKGGTANSSAAELSGIVHIGPSLEYLERAADDAKYGRFSKHPFLELTIPSL
                     VDPSLAPAGKHVMSVWMQSAPYHLKESNWDDQRQALGDSVTDIIETYAPGFKNSILHR
                     QVLTPLDIEQVFGVTGGHLDHAELALDQIFFMRPVPGWAGYSTPIENLYLCGSGTHPG
                     GGISGLPGYYAAKKILRSWPRDK"
     gene            complement(2024..2446)
                     /locus_tag="VF089_08885"
     CDS             complement(2024..2446)
                     /locus_tag="VF089_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6174116.1"
                     /translation="MQSLEKFFKSAVLVLGALLVFGCAQRVALRNAPDVPAAVGEVKI
                     TRDQNRNSIVDLEIQHLAPSQNLKPPKKFYVVWSQAPDGKNINLGQLTVGSDRRGTLK
                     SPTPMQVFRIVVTAEDVPTAAYPSSQIVFSSDYVRTGG"
     gene            complement(3139..4203)
                     /locus_tag="VF089_08890"
     CDS             complement(3139..4203)
                     /locus_tag="VF089_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6174117.1"
                     /translation="MMNKNNSTDRDSGFTGFRRRVLFVAGTVVFVVLILALLWLAGNV
                     FLLIFAGILLAVFLRSISDWISNHSFLSDGWALAVTVLGLLLLLGAGAWLIGSDVVTE
                     AGQLRQQLPQSLERLKQNIEQYEWGRQLLGQTSRADELIPEKRELVAKATGVFSTTFG
                     ALAMLVVFLFLGLFLAATPELYVSGVIRLIPINKRARAHQVLRAAGSALRWWLLGKIA
                     AMIVVGVLTALGLAWLGVEPALTLGLLAAVLTFIPNFGPILSALPAVLLALTDDPVKA
                     LYVIILYVAIQTVESYVLTPLVQQRTVSLPPALTITAQLFLGIFAGALGVIVATPLTA
                     ALLVSIRMLYIEDVLGDEEA"
BASE COUNT         1137 a         1283 c         1227 g         1098 t
ORIGIN      
        1 ccctatcatt tgaaaagtgg gacttgggcc gagaagcgcg agcaattcgg cgatacggtg
       61 atagacacaa tcagcgagta cgctcccaac ctaaaagata tcatcttgca tcgtcagatc
      121 gtcacgccgt gggatcttga agacgaattc ggtctcaccg agggaaatat ttttcagggc
      181 gagctaaccc tcgatcagct tttcttttta agaccggtgc caggttggag cagatatcgc
      241 acgccgataa agaatctcta catgtgcggc gcctgcacgc atccgggcgg aggagttatg
      301 gcggcatcgg gcagattggc agcgctcgag atcttaaagg atgtaaaaag gagcttcatc
      361 taaccgccgt gcagcattat gacgcgatca tcgtcggcgc cggccataat ggactggtta
      421 cggcggctta tctagccagg tcaggtaaaa aggtattggt tctagagcgg cgatcgatga
      481 tcggcggtgt cgccgccacc gaagaagtct ttccagcttt caagtgctca accggcgctc
      541 atcttgccgg atcattttcc cgggagatca gcgccgaact ggaactacag aaacatggct
      601 tgaagcttgt ctctgtcgat ccgctagttt tcgcgccaca aatagacggc acttcgctgc
      661 tgcttgcgtc agatcccgcc aaaatcggcg cagagatcgg ccggttttcg cgcgcggatg
      721 ctgagaaatt ttcttcgttt tgctcgctca tagataaact cgcccggttc ctgcgagtgc
      781 taaacgcgct gcccctaccc gatggcacaa gcccccgcag tttgaatctc tcggagctgg
      841 ttaaaatcgg ggtgaaattt catcgcctcg gcaagaaaga gatgtatgag ttcttacgca
      901 ttctgcccat gagcattgcc gacctgctgg gcgaatggtt tgaaaacgaa accttgaaag
      961 cggccatagc cgccggcgcc attgtgaact ctttcgtggg tccgcgcgcg cagggaacgg
     1021 cttatgtgct tctgcatcat ctgtcgcaac aagccaacgg cccttttcgc agcgctgggt
     1081 tcatttccgg cggcatcaat cagttgcccc aggcactggc gcgggcggca aggcagcatg
     1141 ccacggaaat ccgcaccggg gccgaagtcg ttcgtatcat caccaaagat ggtgcagcca
     1201 aaggcgtggt tctccagaac ggcgatgatt tctcagcgag cgtggtgatt tccaacgccg
     1261 atcccaagcg gacgctgctc cacttggtgg aaccgacata tctcgatccg catttcttgt
     1321 tgcaaattaa aaacattcgc gcgcgcggca ccgtcgccaa agtaaatttc gcattggata
     1381 cgttgcctca gttcaaaggc gggactgcta attcttcggc cgcagagcta agcggcatcg
     1441 ttcacatcgg tccgtcgctt gagtatctcg agcgcgccgc tgacgatgcg aaatatggca
     1501 gattttccaa gcaccccttt ttagagctaa cgattccgtc gctggtcgat ccatcgctag
     1561 cgccggctgg aaagcacgta atgtcggtgt ggatgcaatc ggcgccctat cacctcaaag
     1621 aaagcaattg ggatgatcag cgccaagcgc tgggtgacag tgtgaccgat ataatagaaa
     1681 cctatgcgcc cggatttaaa aactccatct tgcacaggca ggttctaaca ccgctggaca
     1741 tagaacaagt attcggcgtc accggcggtc accttgacca cgcggagctg gcgctggatc
     1801 agatcttttt tatgcgcccg gttccgggct gggcgggata cagcacgccg atcgaaaact
     1861 tgtatctctg cggctcgggg acccatcccg gcggcggaat ctcgggccta cccggctact
     1921 acgccgcaaa aaaaatcctg cgtagctggc cacgagacaa gtgatctcgc cccctcccac
     1981 tggcagaggt tcatgtgagt tgaacatgcg tgccgcgaca tgctcaacct ccggtcctta
     2041 cgtagtccga cgagaagaca atttgagaac ttggataggc tgcagttgga acatcttcgg
     2101 cggtcacgac aatccgaaac acctgcatgg gagtcggact ttttaatgtc cctcgtcggt
     2161 cggaaccaac cgtgagttgt ccgagattga tattcttgcc gtccggtgct tgggaccaga
     2221 ccacatagaa cttcttgggc ggctttaggt tctgagaagg agccaagtgt tggatctcca
     2281 gatcgacaat tgaatttcta ttttgatcgc gggtaatttt aacctcaccg acggccgctg
     2341 gaacgtccgg cgcatttcgc agcgccaccc tctgagcgca gccaaaaacg agtaaggcgc
     2401 ctaacaccag tactgcggat ttgaaaaatt tctctaagga ctgcattgtc attgtcttcc
     2461 cccgtccatg tagttatgtt tcaacttgtt ttgtttcttt gtgagaaatt cttgagcaat
     2521 aaccgtaccc ggtcctggca gtacctatcg aaagagttga agagcctggc gtggtgagaa
     2581 cttcgatagt ttttatccaa cttgtaaagg attttttttg acaagctctc gctcacccgt
     2641 atatccgcta tcccaaccat ctctcaacgt gtcccacttt gccaaacgca ggagcgcgcc
     2701 ccgtcctaat tttgagcggg ggctacgcaa ctcaagctgt gcgcttcagc atttgcgtgg
     2761 cagtacggtc gtcactgatt tcagttggct tatcgtagag ggattcatcg gagatctgtt
     2821 aagggacttc ggccaactag ttggctccac ctcaatatac ctcacgagtg ctgcaccgtg
     2881 atctctataa tgagaggagc tctggctgat actctcgaag ccattggtta tacgcatgta
     2941 atctcgtaac gacggatgcg gctctatcga agtgcgcctg tgaatcactc cagcagcaat
     3001 accaaaggct catgcaccag aaggtatgat gcaaaggttc aacgaatcga tcaattccgc
     3061 ttgtgcgagt ggctgaggct taaacgcgta cagctactaa gcattcggtt gtgggtgtgt
     3121 caggtgcgca tgcacggatt atgcttcctc gtctcctagg acatcctcga tgtacagcat
     3181 tctgatcgac actaagagcg ccgccgtaag cggcgttgcc actataacgc ccagtgcccc
     3241 agcgaaaatt cccaaaaata gttgcgcggt gatcgtcagc gccggcggca atgaaaccgt
     3301 acgttgctgg actagggggg ttagcacgta gctttcaacg gtttggatcg cgacatacaa
     3361 gataatgacg tacagagcct tcaccggatc gtcggtcagt gccaaaagaa ctgccggaag
     3421 tgccgacaaa atcggaccga agtttggaat aaacgtcaat acggcggcga gcaatccaag
     3481 agtcaaagcg ggttctaccc ccagccacgc caggcccaac gcagtgagaa ctccgacaac
     3541 aatcattgcc gcgatcttgc ccaacagcca ccaccggagg gcactgcccg ctgcccttag
     3601 aacctgatga gcgcgtgcac gtttattgat cggaatgagc cgtatcactc cactgacgta
     3661 aagttcagga gtagcggcta gaaacaatcc aaggaacaga aacactacca acatggcgag
     3721 tgcaccgaag gtggttgaaa aaactcccgt tgctttagcc acgagctctc ttttttcagg
     3781 aatcagttca tcagctctgg aggtttggcc caggagctgt ctcccccact cgtactgctc
     3841 aatattttgc ttcaagcgtt ccagtgattg cggaagttgt tgccgcagtt gaccagcttc
     3901 cgttacgaca tctgagccaa taagccatgc tcccgctccc aacagcagaa gtaatccgag
     3961 cactgtgacc gccagagccc aaccgtccga aaggaagctg tgattgctta tccagtcgct
     4021 aatggaacgt aaaaagactg ccagcaaaat cccggcaaag atgaggagaa aaacgttgcc
     4081 tgcgagccac aacagcgcca atatgagaac aacaaaaact acagttccag ctacaaacag
     4141 cacgcgcctt cgaaatcccg tgaatcccga gtctcggtcg gtcgaattgt tcttattcat
     4201 catttgactt tgccatatgt tttctccctc agctaacttc cgccgctgac ctgtgaagta
     4261 acgaaccgcg gttgaagcag tttgtgccga atctcagagt ctcatccgcc tctcctccat
     4321 gtctgattgc gcctcgcaca ggttcacagt gattcctagg ctagccgtag ctgatcttca
     4381 gtaagcgagg aaataactcg agcctctact aagtcggctt catctgagcg gtcagaaatc
     4441 agccggatag tgtggcaagt actagagtag ccataatcga cgtgggccaa tagttcagtt
     4501 cggtgcagtg acgcttttgc tgtaaattcc agtcactcat ttgttcctcg agttagtgtg
     4561 tgtgccgaca tgccatcacg gagcaatcga tacaccagtg aaggtcggca ggagcgactc
     4621 tttcactcgg tccgtccata ctcgattgcc ctgccggcgc aaccgtcgaa tcaagctggt
     4681 tatttgccga acgggagatg acactggatg agaactgata tataagcgtc gcgtacaaca
     4741 cggtt
//
LOCUS       DASUTG010000386         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1396416, whole genome shotgun sequence.
ACCESSION   DASUTG010000386 DASUTG010000000
VERSION     DASUTG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396416"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1209
                     /locus_tag="VF089_08895"
     CDS             109..1209
                     /locus_tag="VF089_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174118.1"
                     /translation="MSEQAIRVIDIDGHVREADDLWERYLEQPFRARAPKIERVANGQ
                     LLFLLEGKRHHRKPDETPFRQKDDGSPVNEHRDLATDPRARLKDMDRDGIERGLLFPS
                     AGLYLTSVEEESYAAALCRAYNNWLYDYCGADRARLMAVGAIPVQDVNAAVAETRRVV
                     NELGFKAIFVRPNPVKGRTLDDPYYDPLYDTLEELGVPLMVHEGSGAYLPTAGADRFA
                     GQWFFTHTVSHPFEQMLASLGLICRGTLERHPELQVVFLEAGAGWLPYWLWRMDEHYE
                     ILPFQVPWLKMKPSDYFRRQCFTSCEPDETRLGEVIESIGAGRVLFASDYPHWDAKLS
                     GSPRAVIDRADIAADTKRRIMGENAARLLRLD"
     gene            1302..>2045
                     /locus_tag="VF089_08900"
     CDS             1302..>2045
                     /locus_tag="VF089_08900"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX6174119.1"
                     /translation="MAETQKLVSSMGKAELDKIFVRGYDLNEQLLGKISFAQMVYLMI
                     AGRMPTAEEGRMIDTMLIVLVDHGMTTGAAAARMTFHSAPEAIQGAVAAAILGAGSVH
                     LGSSEHCAKMLHDALPPDSKATDLDAIARAAVEKRLANKQVVPGIGHGIHTGGDPRAE
                     RLFQVAQETKVYGRYCELLRKIGKVADERVGRHLPVNVTGAIAAISLDMGIHWQMSKS
                     FAILGRALGGLAHVGEEIRRPIARGISNLI"
BASE COUNT          438 a          568 c          606 g          433 t
ORIGIN      
        1 ggaagcaatc aaaaccgttc tcgaccagca gggcaaacct gacctgccgt tggatcgggt
       61 ggtggattcc agcattgtcg aagaggtcct gcgcgaacgg agataagtat gtcagagcaa
      121 gccatacgcg ttatcgatat cgatgggcat gtccgagaag cggacgattt gtgggagcgt
      181 tatctcgagc agccgtttcg cgcccgcgcg cccaagatcg agagagtcgc caatggccag
      241 ctgctttttc tcctggaagg caagcgccat catcgcaagc ccgatgaaac gccctttcgt
      301 caaaaggatg acggctcacc cgtcaacgag catcgcgatc tggcaaccga tccgcgcgcc
      361 aggctcaaag atatggatcg tgacggcatc gaacgcggtc tattgtttcc atcggccggc
      421 ttgtacctga catcggtgga agaggaaagt tatgcggcgg cattgtgtcg cgcctacaac
      481 aattggctct atgattattg cggcgctgat cgcgcgcggc taatggctgt gggcgccatt
      541 cccgtgcagg acgtcaacgc cgcggttgcc gaaacccggc gcgtggttaa cgaactcggt
      601 ttcaaggcga tttttgtccg gcccaatccg gttaaaggcc gcacccttga tgatccctac
      661 tacgatccgc tgtacgatac gctggaagaa ctgggggtgc cgttgatggt gcacgagggc
      721 tccggtgctt atctgcccac agccggtgcc gaccgtttcg ccggtcagtg gtttttcacg
      781 catacagttt cccatccgtt cgagcagatg cttgcgagtt taggtttgat ctgcagggga
      841 actttggagc gccatcccga actgcaagtt gtctttctcg aagccggcgc cggctggctg
      901 ccctactggc tatggcgcat ggatgagcat tacgagattc ttccgtttca agtgccatgg
      961 ctgaaaatga aaccgagcga ctatttccgc cgccagtgct tcacctcgtg cgagccggac
     1021 gagacccgct tgggcgaagt catcgaatcc attggcgcag gacgggttct gttcgcatcc
     1081 gattatccgc attgggatgc caagctgtcc ggttcgccac gagcagtcat cgatcgcgca
     1141 gatatcgccg ccgacaccaa aagaaggatt atgggtgaga acgccgcccg gctgttgcga
     1201 ttggattagc gcgacctgtt ggtatgtttg tctctatcgt tccgcttacg tgatccgggt
     1261 attctgaata agttcgtttc aacaagaaga atggaggatt aatggctgaa acacaaaaat
     1321 tggtctcatc gatgggcaag gccgagctcg ataagatttt cgtgcgcggc tacgatttga
     1381 atgaacaact gctcggcaaa atctctttcg cgcaaatggt ttatttaatg atcgccggcc
     1441 gcatgccgac agcggaagaa ggtcgcatga tcgataccat gctgatcgtg ctcgtggatc
     1501 acggcatgac cacgggcgca gcagcggcgc gcatgacctt tcactccgcc cccgaagcga
     1561 tccaaggcgc cgtcgcggcg gcgattctcg gcgccggcag cgtacatctg ggatcttcgg
     1621 agcactgcgc gaaaatgctt cacgatgcgt tgccgccgga ctcgaaagca accgatctcg
     1681 acgccattgc cagagcggca gtggagaaac ggctggctaa caagcaagtg gtgccaggca
     1741 tcggccatgg cattcacacc ggcggcgatc cgcgcgccga gagactcttt caagttgcgc
     1801 aagaaacaaa agtttacggc cgttactgcg agctgctgcg aaagatcggc aaagtcgcgg
     1861 acgaacgggt gggcagacat ctaccggtaa atgtcaccgg cgccatcgcg gcgatctcgc
     1921 tcgacatggg tatccattgg cagatgtcga agagcttcgc gatcctcggc cgcgcgctgg
     1981 gcggcctggc tcatgtcggc gaagaaatcc gccggccgat cgcccgcgga atctctaact
     2041 tgatc
//
LOCUS       DASUTG010000387         5587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_61891, whole genome shotgun sequence.
ACCESSION   DASUTG010000387 DASUTG010000000
VERSION     DASUTG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61891"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            715..1371
                     /locus_tag="VF089_08905"
     CDS             715..1371
                     /locus_tag="VF089_08905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6174120.1"
                     /translation="MAVRINDVAPDFTAETTEGKINFHEWIGDSWAILFSHPKDFTPV
                     CTTELGYMAKLKPEFDKRNCKIIGLSADPLTDHQGWVKDIQETQGHAPNYPMIADPEL
                     NVAKLYDMLPASETPGKRTAADNQTVRNVFLIGPDKKIKAMLIYPMASGRNFDEVLRL
                     LDSLQLSVKHTVATPVNWKPGDDVIIPTSVSDEEAKKKYPQGFKTHKPYLRTVSQPGK
                     "
     gene            complement(1486..2145)
                     /locus_tag="VF089_08910"
     CDS             complement(1486..2145)
                     /locus_tag="VF089_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6174121.1"
                     /translation="MALELYWGSGSPFAWRVMLTLEVKRLTYQSTLLEFSKGEHKSSG
                     FLKLNPRGKVPVLKDDDFVLNESLAIIAYLDKKYPDPPLFGTSPQETGLIWRAILETE
                     AYLLSAGDKVIRPVFFGKGLDETAQIQEAAQAVREELKRIDRELSNSPWLVGKQLSAA
                     DISLFPLVQTILRAAGKDAAKPLKLELLPLADTFPNLSAWVKRIEVLPGYERTYPPHW
                     R"
     gene            complement(2455..3471)
                     /locus_tag="VF089_08915"
     CDS             complement(2455..3471)
                     /locus_tag="VF089_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174122.1"
                     /translation="MKSLRNTFVNCIFWLVLMALIAASDGAGNAAESAKFARLRLAYS
                     AITVNQAIPWISLEAGHFKKHGVDVELIHASSITALQALLAGEVTIVQSATDGAVSSN
                     LSGADTVFMGAILDKPLYSFIVNSKIKTPQDLKGKRVGVTRFGATPDALARATLKMWG
                     LDPSTDVSMIQLNEMGLLVQGLVNGVIDAAPISIPNNIRAKNLGFVELFDMTKIGKTY
                     ITGTVVTRKRFIDGQRDLAKRFMRGFLEGMKTYQEDEEFTIKVIQKWTRAKNRDEVKE
                     AYALQAKNMLRVPRTSVDGVRTILEGLDKMPAAKTADPRRFIDFTILDELEKEGFLQS
                     LYKN"
     gene            3764..5545
                     /locus_tag="VF089_08920"
     CDS             3764..5545
                     /locus_tag="VF089_08920"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6174123.1"
                     /translation="MADAISHDARWSEYPGDEWSDAMVQAMKLGGIDHLFFVSGSEIA
                     FWQESIAKAQERDWPAPKLITVPHESVALNAALGSAMIRNQPAATAVHVDVGTLNYGG
                     AIHTAWRGNYPVLITAGTGPRAYAGSMTGARNSPVQWVQEPRDQGGIVRQYTKIDHRL
                     EHQDNPGFMISRLLQVAMSEPKGPVYLAAPRETAMMKLPGKTYFPTRDELGIARPVWP
                     DPTDARQAAQWLIEARNPLICCATGGRNPEAVAETVRLGELLALPVVDCDRVDRLNFP
                     STHSLYGTGPEIKDVDAVLMLEALVAYTTPEAAPRPGSKVIWADADPVMSAYKTMEFQ
                     ADLWLPVTAATAARAIYEAATALLSKSDMSRIAERRERLEASKREMIHNNEQAAIAAG
                     RRRPMHPRWVAYQLGKVLEPNAIVLDDSISNSDFVCAHHGRTQAGTYFASGGSSGGWG
                     AGAALGAKLAAPERDVILTTGDGYFMFGTPMPALWTAAHYKAPFLTLVFVNRSYSTGT
                     NGLRRTYPSGVAVRTENFEGGVFDPPPDFAKLAESANCYGETVREPDEVGPALKRGLE
                     QVRKGTPALVAAYLPTLVEEMSLAARK"
BASE COUNT         1312 a         1589 c         1459 g         1227 t
ORIGIN      
        1 cacagcgtaa attgcagttt gttacccgta ttcagttgtc aaagaacatc ccacagctca
       61 gatctctctg aaccgctaac ccctaaaaga gatcagtaaa cagtgatcct tacccagaac
      121 ctctctttag caacctctag cagcagttct tttatacgaa cgagcggcca ataaccttcg
      181 cccgctacct gagattcaat ctttcaaaac taaatagtcg ctgctggagt tggcctgatc
      241 tcagccccag cctgctgatg agactggaca cgtttccccg ctgaataatt tgggaatgac
      301 tgtcactgct ctctaattag ctttcaaaga tcgaactctc ccgggcggga gagcttcagc
      361 atccgctgga tgctattggt cagtacgatt aaaacccgaa acctggcgct gcgatttcgg
      421 accgggaaaa tcaatctaag atttggatgg atccatgtca agtagtcctg tccgtgtata
      481 gataattcgc tctctgtcga cagattttct atgcgcgtca tcctcggcaa tccagtctca
      541 tggctcggtt tcgcatatta cctgaccccg tcgcaagacc cgacccgagc gaatttccct
      601 ccgtctcaca aagccaccga atgactgcca atgtcttgaa aaaatattga ctgtcgttcg
      661 tattcgcgat agctctatcc cacccaaaaa actataacga agggagaaca aaccatggct
      721 gttcgaataa acgatgttgc accggatttt accgcggaga caaccgaagg caaaatcaat
      781 ttccatgaat ggatcggcga tagctgggcc atcctatttt cgcatccaaa ggatttcacg
      841 ccggtctgca ctaccgagct tggctatatg gccaaactaa aacccgaatt cgataagcgc
      901 aactgcaaga tcatcggact gagcgccgac cccttaaccg atcaccaggg ttgggtcaag
      961 gacattcagg agacgcaggg gcatgcgccg aattacccga tgatcgccga cccggagtta
     1021 aacgtcgcca aactttatga catgctcccg gccagcgaga cccccggcaa acgcaccgcg
     1081 gcggacaacc agactgtccg caacgtattt ctcatcgggc ccgacaagaa gattaaggcg
     1141 atgctgatct atccgatggc cagcggccgc aacttcgatg aagtgctgcg tctgctcgac
     1201 tcgctgcaac tcagcgtcaa gcacacggtc gcgacgccgg ttaattggaa gccgggcgac
     1261 gacgttatca ttccgacatc tgtttccgat gaggaagcca agaaaaaata cccgcagggc
     1321 ttcaagacgc acaaacctta tctgcgaacg gtatctcagc ctggcaagta accacttggc
     1381 tccttgacat ctgagaaggt cgcattgtaa tccctggtgg ctgctgctga ttaacacggc
     1441 agcagccaca gccaatttct atcgagtacc gaagaaaaga tctgcttatc gccaatgcgg
     1501 cggataggtg cgctcataac ccgggagcac ttcgatccgc tttacccatg ccgaaagatt
     1561 tgggaaagta tcggccagcg gcagaagttc cagcttcagc ggttttgccg cgtctttgcc
     1621 cgccgctcgc aggatagttt gaaccagagg gaacaagctt atgtccgcgg cagagagctg
     1681 tttgccaacg agccacggcg aattcgagag ctctcggtca atgcgcttca attcctcccg
     1741 gacagcttgc gccgcttcct gaatttgcgc tgtttcatcc aaccccttac caaagaaaac
     1801 cggccggata actttatcgc ctgcactgag caaataagct tccgtctcca agatggctcg
     1861 ccagattagt ccagtctctt gcggcgaggt accgaacagc ggcgggtcgg gatatttctt
     1921 atccagatac gctatgatcg ccagcgactc attcagaacg aagtcatcat ctttcagcac
     1981 aggcactttg ccgcgcgggt tgagtttaag aaaaccggaa gatttgtgtt caccctttga
     2041 gaattccaag agggttgatt gataggtgag gcgtttcact tccaacgtca gcatgacgcg
     2101 ccaggcaaac ggactgccgc tgccccagta gagttccaaa gccatctcgc acctccttta
     2161 aatcgtccaa tcatttaacc gaattcgatg ttccatttaa aagttggcag ttatatcgtt
     2221 tatggatcgc aaggcttccc agggatcggt ctcgcccaat caccgccaaa agcggcaccg
     2281 tcgttacagt taagtgatat cgctgatcaa agttcatctg gcagaaaata tcgccaagga
     2341 gcggaaagca ggctagcccc tgccgcttag atttgcttcg aatccgatgc ttggcggtct
     2401 tcggaagctc tgctccgcgg gcgcattccc tacatagaca aataactttc tcgatcaatt
     2461 cttgtagagc gattgcaaaa atccctcttt ttccagttcg tcgagaatcg tgaaatcgat
     2521 gaagcgtcgc gggtcggcgg tcttagccgc cggcattttg tcgagaccct caaggatggt
     2581 cctcacgccg tctacggatg tgcgcgggac tcgcagcatg ttctttgcct gtaaggcata
     2641 ggcctccttc acttcatctc tgttttttgc gcgcgtccat ttctgaatca ccttgatagt
     2701 gaactcttcg tcttcctgat aggtcttcat gccttcgagg aagccgcgca tgaaacgctt
     2761 ggcgagatcg cgctggccat cgatgaaacg ttttctcgta accaccgtgc cggtgatata
     2821 ggttttgccg atcttggtca tgtcgaataa ctcgacgaat ccgagatttt tcgcccggat
     2881 gttattgggg atcgatattg gcgcggcatc gatcacgccg ttgaccaatc cttgcaccaa
     2941 caggcccatc tcattgagtt gaatcatcga gacgtcggtg gacggatcca acccccacat
     3001 tttgagcgtg gcccgggcca gagcatccgg tgtcgcgccg aatcgggtga ctcctactct
     3061 tttgcccttt agatcttgcg gcgttttgat cttggaattg acgataaagc tgtagagcgg
     3121 cttgtccaga attgccccca taaaaacggt atcggcgccg cttagattgg aactcacggc
     3181 gccatccgtc gcggattgca cgatcgtcac ctccccggcc aacagcgcct gcagcgcagt
     3241 gatactgctg gcatgaatca gttccacgtc gacgccgtgc ttcttgaaat ggcccgcctc
     3301 taaactgatc cacggaatcg cttggttgac cgtgatagcg ctgtatgcaa ggcgcaatcg
     3361 ggcgaacttt gcggattcgg cagcgttgcc ggctccatcc gaagcggcaa tcaatgccat
     3421 gagaaccagc caaaatatgc aattcacgaa cgtgtttcga aggctcttca tacatcctcc
     3481 acctttcgct cttagaaata ttcgacttct ccgccttaga tcaagtggat tttcctcttg
     3541 gtcattacct aatctttgaa cactgcatcg cgctagatca ccgatgagag cattcaagaa
     3601 taacgcaccg agcctggaga aatgttttaa acaacgcgcg ggctccaatc aaaccggcaa
     3661 ttattgcacg gcgttgaaaa aatggttgcc aagcgactgc gctatggggg atagtgccgg
     3721 tggacctttc aaaacagata atcctccaag gagagctacg ttgatggcag acgcaatttc
     3781 ccacgatgcg cgctggagcg aatatcccgg cgacgaatgg tccgatgcca tggtccaggc
     3841 aatgaagctc ggtggaatcg accatctatt ctttgtctcc ggctccgaga tcgctttctg
     3901 gcaggaaagc atcgccaagg ctcaggagag agattggccc gcacccaagt taattacggt
     3961 gccgcacgag agcgtggctc tgaacgctgc tctcggcagc gccatgatca ggaatcagcc
     4021 ggctgccacc gccgtgcatg tcgacgttgg aacattgaat tacggcggcg cgatccatac
     4081 cgcctggcgc ggtaattatc ccgtgctgat caccgctgga acaggaccgc gggcttatgc
     4141 cggatcgatg accggcgcgc gcaacagccc cgtacaatgg gtccaagagc cgcgcgatca
     4201 gggcggtatc gtcaggcaat acactaaaat cgatcatcgc ctggagcatc aggacaatcc
     4261 aggattcatg atcagccggt tattacaagt cgccatgagc gagcccaaag ggccggtgta
     4321 tcttgccgcg ccgcgcgaga cggcaatgat gaaacttccc ggtaagacct acttcccgac
     4381 ccgcgacgag cttggcattg cccgaccggt gtggcccgat cctaccgatg ccaggcaggc
     4441 cgcgcagtgg ctcattgaag cgcgcaatcc gctcatctgc tgcgctaccg gcggacgcaa
     4501 tcctgaagca gtggcggaaa ctgttcggct gggcgagtta ctggcattac cggtagtcga
     4561 ttgcgaccgc gtcgaccggt taaacttccc aagcactcac tcgctttacg gtaccgggcc
     4621 ggagatcaag gacgtcgatg cggtgctgat gctcgaggcg ctggtggctt acaccacgcc
     4681 tgaagccgcg ccacgaccgg gcagtaaagt tatctgggcc gatgccgacc cggtgatgtc
     4741 ggcgtacaag accatggagt tccaagccga tctctggctt ccggtgaccg cggcgaccgc
     4801 ggcgcgcgcc atctatgaag cagcaaccgc cctgctttcg aagagcgaca tgagccgaat
     4861 cgccgaacga cgtgagcgat tagaggcaag caagcgcgaa atgatccaca acaacgagca
     4921 agccgccatc gcagccggac ggcgcaggcc catgcacccg cgatgggtag cctatcaatt
     4981 gggaaaggtc ctcgagccga atgccatcgt gctcgacgat tccatcagca actcggattt
     5041 cgtctgcgcg catcacggga gaacacaagc cggcacttac ttcgcgagcg gcggatcatc
     5101 cggtggatgg ggggccggcg cggccttggg agccaagctc gccgcaccgg aacgtgatgt
     5161 catcctgacc accggcgacg gatactttat gttcggcaca ccgatgccgg cactgtggac
     5221 ggccgctcat tacaaggcgc catttctgac attggtgttc gtcaaccgct cttacagcac
     5281 gggaactaat ggcctgcgca gaacctatcc gagcggtgtc gcggtgcgca cggagaattt
     5341 tgaaggcggc gtattcgatc caccacccga ctttgccaag ctcgccgagt ccgccaactg
     5401 ctacggcgag accgtacgtg aaccggatga agtcggcccg gcgctcaaac gaggattgga
     5461 acaggtgcgc aagggcactc ccgcgttggt cgcggcgtat ttaccgactt tggtcgagga
     5521 gatgtcattg gcggcgcgga agtaggaata cgttcaattc aagggttcaa agatggcggc
     5581 aaaactt
//
LOCUS       DASUTG010000388         3138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_210191, whole genome shotgun sequence.
ACCESSION   DASUTG010000388 DASUTG010000000
VERSION     DASUTG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210191"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="VF089_08925"
     CDS             complement(<1..1403)
                     /locus_tag="VF089_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174124.1"
                     /translation="MKTYRRLLPFLKPYIWPYFVLGIVCMLGYSATGGVIPFLVQSVF
                     DDLFQRKEVSMLYYLPLAIIILFTFRGLLNFGQHYFSDYVGLRIINNVRNELNRHLQY
                     MSLSFFYRHPTGTLLSRVGNDVGLLRSVLTDTLASLVRDSVSVAVLIGVAFYKDWVLA
                     SIAFFVFPASVLPVMRLSRKIKRFSKRGQISTGNLTALLQESIQGNRIVKAFGMEEYE
                     IRRFTAENFHLFKQSLRATRIKAVVTPAMELLASFGIAGVVWYGGLSVISGGRTQGEF
                     LAFMTAMMLMYQPFKSLAKTYATVQQGIAGADRVFEVLDVTPEVKERAGAREAHRFAE
                     SIEFHDVSFGYNEKLVLKNINLRICAGEMVALVGVSGVGKSTMADLIPRFYDVTSGAI
                     TMDNIDIRDLTFRSLRQQIGLVSQHTFLFNDTIGNNISYGDPDRGLDDIIAAAKAAYA
                     HDFIMALPKGYDTEIGE"
     gene            complement(1417..2568)
                     /gene="lpxB"
                     /locus_tag="VF089_08930"
     CDS             complement(1417..2568)
                     /gene="lpxB"
                     /locus_tag="VF089_08930"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX6174125.1"
                     /translation="MTRGKEIMLVVGEASGDIHGASLVKALLKRDPNLTFFGVAGDQL
                     QQTSFEPLFTVSELTGMGLLELAGNIKNIVAAYRLLRRTLRTRRPSLLILIDFPELNL
                     RLAKLAKSLGVPVLYYVSPQVWAWRRRRIRQIARVVDKMAVVFSFEVEFYKRHHMPVE
                     FVGHPLLDVVRVSQERDPTLREIGLHKEKPIIALLPGSRRKEIAYHLPVMVAAAAELK
                     RTEQAQFFCVRASTLDRAELQKELDRADFVIPIVEAGRYNAVNAADLVWAASGTATLE
                     TALLEKPMIVVYRVSWVTYWLARLLVKIKYIGIVNIIAGKAIVPELVQSDLSPERLVR
                     ESQAILNDHDARRTMIANLRDLREQLGVPGAANRVAELAISLMAPVRAC"
     gene            complement(2595..>3138)
                     /locus_tag="VF089_08935"
     CDS             complement(2595..>3138)
                     /locus_tag="VF089_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6174126.1"
                     /translation="IRRLEGVIKEPKFVECHRLAPFVERGTDVDVVLDLMIHDIDVIA
                     SLVRSPVTRVEAVGVPVLTETPDIANARLIFANGCIANVTASRVSVKRERKVRFFQPD
                     AYISIDYDQRRAQIIYKPEPGAGWLDVRSENIEFKEGDALADEIDSFLDCVRTRAAPL
                     VSGNDGLNALEIASLIRSQL"
BASE COUNT          768 a          888 c          801 g          681 t
ORIGIN      
        1 atttcgccaa tctcggtatc atagcccttg ggcaacgcca tgatgaagtc gtgggcgtag
       61 gccgccttgg ccgcggcgat gatatcatcc aggccgcgat cagggtcgcc atagctgatg
      121 ttgttgccta tcgtgtcgtt aaacaaaaaa gtatgctgac ttaccagacc gatttgctgc
      181 cgcaatgatc ggaacgtgag atcccggata tcgatgttgt ccatagtgat ggcgccggac
      241 gtgacatcgt agaagcgcgg aatcaggtcg gccatagtac ttttgccgac cccgctaacc
      301 cctaccagcg cgaccatttc accggcgcag atacgcagat tgatattctt taagacgagc
      361 ttttcattat agccgaagct cacgtcatga aactcgatgg attcggcaaa tctgtgcgcc
      421 tctcgggcac ctgctcgctc tttaacctcc ggggttacgt ccaaaacctc gaacactcga
      481 tcggccccag cgatcccttg ctgaacggtc gcgtaggtct tggccaggga cttaaaaggc
      541 tgatacatga gcatcatcgc cgtcataaat gccaggaact cgccctgagt tcgcccgccg
      601 ctaataacgc ttaagcctcc gtaccacacg actcctgcga tcccaaagga ggctaacaac
      661 tccatcgccg gagtgactac cgccttgatt cgagtcgcgc gcagagattg cttgaacagg
      721 tgaaagttct ccgcggtgaa acgtctgatc tcgtactctt ccatgccgaa tgctttgacg
      781 attcgattgc cttggatact ttcttgcaaa agcgccgtta aattcccggt gctgatctgc
      841 cctcgtttac taaaccgctt gatttttctg ctcaagcgca ttaccggcag aaccgacgcc
      901 ggaaatacaa agaatgctat cgaagccaaa acccagtctt tgtagaacgc aaccccgatc
      961 aatacggcta ctgagacgct gtctcgcacc agagaagcca acgtatcggt gagcactgac
     1021 cgcaacaagc caacgtcatt accgacccgc gacagcagag tcccggtagg gtgccgatag
     1081 aaaaaagaca acgacatata ctgcagatga cgattgagct cattgcgcac gttgttgatg
     1141 atgcgcaatc cgacataatc gctgaaataa tgttgcccaa aattaagcag gccgcgaaag
     1201 gtaaacagga tgatgatcgc taaaggaagg taataaagca tgctcacctc ttttctttga
     1261 aagagatcgt cgaacacgct ttgtaccagg aatgggatca ctccgccggt ggcgctatag
     1321 cccagcatgc agactatgcc gagcacaaaa tacggccaaa tatacggctt taaaaaaggc
     1381 aataatcgtc gataggtttt cacgcgaatc tcaatctcaa caagctctca ctggcgccat
     1441 caatgaaatg gcgagttcgg caacccgatt ggccgccccg ggaacaccga gttgctcccg
     1501 gaggtcacgg aggttcgcga tcatcgtccg gcgggcatcg tgatcattga gtatcgcctg
     1561 actctcgcgc acgagcctct cgggcgacag atccgactga accaattctg gaacaatcgc
     1621 ttttccggcg atgatgttga cgatgccaat gtatttgatc ttgaccaata gacgtgcaag
     1681 ccaataggtg acccaggaaa cccgataaac cacgatcatg ggtttctcga gcagcgctgt
     1741 ttccagggta gcggtgccgg aagccgccca gacgagatcc gcggcattca cagcgttgta
     1801 acgtccggct tcgactatag ggattacgaa atccgctcga tccagctctt tctgcaactc
     1861 cgctcgatcc agggtgctgg ctctgacaca aaaaaactgc gcctgctcag tgcgcttcaa
     1921 ctcggcagcc gcagcaacca tcaccggcaa atgataagcg atttcttttc gcctgctccc
     1981 cggaagcaat gcgataatcg gcttctcctt gtgcaatccg atttcgcgca acgtagggtc
     2041 acgttcctga ctcacccgga cgacatccag caatggatga ccgacaaatt caactggcat
     2101 gtggtgccgc ttatagaact cgacctcgaa cgaaaatact accgccatct tgtcgaccac
     2161 ccgggcgatt tggcgaatgc gtctgcggcg ccacgcccat acctggggac tgacatagta
     2221 aagcaccggc acaccgaggc ttttagcgag tttggccagc cgcaaattca gttccgggaa
     2281 atcgatgagg atgagaaggc tcggtcggcg tgtccgcaac gtccggcgca aaagacgata
     2341 ggccgcgaca atatttttga tgtttcctgc caactctagg agccccatcc cggtcagttc
     2401 ggacacggta aacaagggct caaaacttgt ttgttgcagc tggtcccctg cgacgccgaa
     2461 gaacgtgaga ttcggatctc gctttagtaa cgctttaacc aggctggccc catggatatc
     2521 gccggaagct tcgccgacca ccagcatgat ctctttgcca cgagtcataa gcgaattatc
     2581 gagcctcggg cagatcaaag ctgagatcga atcaacgacg cgatttccaa ggcgttgagg
     2641 ccgtcattac cgctgaccag aggagcggct cgcgtgcgca cacaatcgag aaacgagtcg
     2701 atctcgtcgg cgagagcgtc tccttccttg aattcgatat tttcactgcg cacgtctagc
     2761 catcccgcgc cgggctccgg cttgtagata atctgcgcgc ggcgttgatc gtagtcgatg
     2821 gagatataag cgtcgggttg aaagaaccga acctttcgtt cccgtttgac cgaaacccgg
     2881 ctggcggtaa cgttggcaat gcagccgttg gcaaagatta gccgcgcgtt ggcaatgtcc
     2941 ggtgtttcgg ttagaaccgg cacgccgact gcctccacac gagttaccgg agaacgcacc
     3001 aggctggcaa tgacatcgat gtcgtgaatc atgagatcca gcacaacgtc aacatccgta
     3061 cctcgttcga cgaaaggtgc caaccgatgg cattcgacga acttcggttc tttgatgact
     3121 ccttcgaggc gccggatc
//
LOCUS       DASUTG010000389         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_210221, whole genome shotgun sequence.
ACCESSION   DASUTG010000389 DASUTG010000000
VERSION     DASUTG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210221"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF089_08940"
     CDS             complement(<1..300)
                     /locus_tag="VF089_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174127.1"
                     /translation="MTPDHTQHTVQTPSAQQPTPTSGDKAGQTHDHKAMQMPDDKTAT
                     SGALMEHQTAQWAHFANMTLGLWLITGVFALGYRSTALQVSDAVSGALVIVLAILS"
     gene            complement(297..1352)
                     /locus_tag="VF089_08945"
     CDS             complement(297..1352)
                     /locus_tag="VF089_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6174128.1"
                     /translation="MRRFAGRFDDVVGFDRKAPNPPPPGCTAVAVDITSDESVREGLF
                     MIREHHGAHVASVIHLAAYYDFFGEPSEQYDEITVRGTGRLLRGLRELDFQVEQFVFS
                     STMLVHRPAEPGQFINEEWAIEPTWAYPESKVRTEQLIREERGGIPAVLLRISGVYDD
                     LCHSIPLAHQIQRIYERQLTSRLYAGSSSHGQSFMHLDDLVDAIAIVVERRAALPPEL
                     PLLLGEPEPLSYDEIQHTLARLIHGEELETLEVPGVLAPLAKAGAWVMQKLPGADRFI
                     KPWMIDRANDHYALDITRARTLLGWEPKRSLRGTIPTMVAALKADPLGFYRENNLELT
                     SELQKQAETPPEGGKSR"
     gene            complement(1722..>2015)
                     /locus_tag="VF089_08950"
     CDS             complement(1722..>2015)
                     /locus_tag="VF089_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174129.1"
                     /translation="AAAVLASSIGVFIIGSLTTLAEISGSLKAALAWSGAVGPLIGKT
                     GVGVIVWLIVWPIFHALWRGKVVNFSVVYTVSVILIALGWLLTFPPVFEAFSG"
BASE COUNT          431 a          613 c          547 g          424 t
ORIGIN      
        1 cgagaggatg gcgagaacga tgaccagtgc gccgctcacc gcatcgctca cctgaagcgc
       61 ggtgctgcgg tatccgagcg cgaagacacc cgtaatcaac cacaatccga gcgtcatatt
      121 ggcgaagtgc gcccactgcg ctgtctgatg ctccatcagc gcgccggatg tcgccgtttt
      181 gtcatcgggc atctgcatcg ctttgtgatc gtgcgtctgc cccgccttgt cgcccgatgt
      241 cggagtgggt tgttgcgccg acggcgtttg gactgtgtgc tgcgtgtggt caggcgtcat
      301 cgggacttac ccccttcggg cggtgtttca gcctgcttct gcaactcgga tgtcaattcc
      361 agattgttct cgcggtagaa accgagcggg tcggctttga gcgccgcgac catcgtcgga
      421 atcgtgccgc gcaaacttcg cttcggctcc cagccgagca aggtgcgggc gcgcgtgata
      481 tcgagcgcgt agtggtcgtt cgcgcgatca atcatccacg gcttgatgaa ccggtcggcg
      541 ccgggcagtt tctgcatcac ccacgcgccg gcctttgcca gcggcgcaag cacacccggc
      601 acttccaaag tctccaactc ctcgccatgg atcaggcgcg ccaacgtgtg ctgaatttcg
      661 tcgtagctga gcggctcggg ctcgcccagc aacaaaggca actcgggcgg cagtgcggcg
      721 cggcgctcga ccactatcgc aatggcgtcc accagatcgt ccagatgcat gaaagactgc
      781 ccgtgcgaag agctgccggc atagagccgg ctcgtcaact gccgctcgta gatgcgctga
      841 atctggtgtg caagcggaat cgagtgacag agatcgtcgt acaccccgga gatgcgcagc
      901 agcacggcgg gaataccgcc gcgctcctcc cggataagct gctcggtgcg aactttcgat
      961 tccgggtatg cccacgtcgg ctcaatcgcc cattcttcgt tgatgaattg tcccggttca
     1021 gccgggcgat ggacaagcat cgtgcttgag aagacaaact gctcgacctg aaaatcaagc
     1081 tcgcgcaagc cccgcagcag tcgtccggtg ccgcgcacgg ttatttcgtc atactgctcg
     1141 ctcggctcac cgaagaaatc gtagtaggcg gcaagatgaa tgactgaggc gacgtgcgcc
     1201 ccgtggtgtt cgcggatcat gaagagtcct tcccgcacgc tctcgtccga cgtgatgtca
     1261 acggctacgg cggtgcagcc gggcggcggc gggttgggcg ccttgcggtc gaagccgacg
     1321 acatcgtcaa atcgcccagc aaaccgccgc ataacggcat cgccgatcgt tccgttggag
     1381 cctgtaacaa tgacgatccc actttccgtt tggttatcac tttccttttt gataccagtt
     1441 tctatattca tagctgtgtt tcccctccga acattcgatt cggtcaaggt ttataaattt
     1501 tggcgagttc gcagatcggt tctgcaacgc tttcgcacgt cgttcaacaa atcatccctt
     1561 tattctttac accccgcaaa atcatccgac ctaaactgcg gccatcatta cgtcggattt
     1621 cattgtcttc ttatgccctt cctgaaatat ccggtaatac ggcgtcgcga cgcgtcccaa
     1681 tcgcttccac ccgataacca aagcgacaaa tactaaattg cttaaccgct gaaagcctca
     1741 aagactggag gaaaagtaag aagccaaccg agcgcaataa ggatcaccga aacagtatag
     1801 acgacgctaa aattcactac tttgccccgc cagagggcat ggaatatggg ccaaacaatc
     1861 agccagacga ttacaccaac gccggtttta ccaataagcg gtccgaccgc cccggaccag
     1921 gccaaagccg cttttaacga tccactgatc tcggccaatg tcgtcagaga accgataata
     1981 aaaacaccga tacttgaggc caacactgcg gctgc
//
LOCUS       DASUTG010000390         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1692168, whole genome shotgun sequence.
ACCESSION   DASUTG010000390 DASUTG010000000
VERSION     DASUTG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692168"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VF089_08955"
     CDS             <1..632
                     /locus_tag="VF089_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6174130.1"
                     /translation="GRVKTGIGQFIDVSLLQVATAFIESHVADYLNGGEAVSRENFPK
                     GRIYSLLDCDKKPFMVHLSGHNQAWEGLLQAAELDSLSGDPRFASRKQRTERHEEIVK
                     ILRQQFAGKPRDYWLPRLDANSIPNAPINTIQEVFEHPQIKHLGIPRQIPHPQMGVTN
                     LVGSPINLSGTPAQFYRPAPLLGEHTEEVLGQLGYDRAALKELRDDRVI"
     gene            788..2002
                     /locus_tag="VF089_08960"
     CDS             788..2002
                     /locus_tag="VF089_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6174131.1"
                     /translation="MDFNVSADPPHVVEFRKEVAAWMAEQFAGHEHLRWSASWSTRED
                     QDEYKFRRGLAEKLGAKGWLFPIYPQKYGGADLTIDHQMVLETELFKYGLNLSHVFYT
                     LARIVAPVLIRWGTEEQKSEFLEPITRAQICVWQVLTEPHGGSDIANCQTKAIRDGDD
                     YVVNGQKVMVGHHLPPDYLWTLVCTTPGGKRHENLAWLHIPANLPGITIQHMHMMMGI
                     KNSVFFDNVRVPAKYLIGGENNGWKVASTHMELEHGGDGRIAADPLIERVVEYCRSTH
                     IDGKPIMEDPRVRDLIAEMTIELNTLRRFNQLIYWRRFAKKPHPYGGPQQRYYQRMLR
                     LRNGERLQAIMGADALEQNLSVHEVVDFEYAVRTGPGQLHGGGTLDTDRLILARRLGL
                     GRPTTEKAPETD"
BASE COUNT          509 a          571 c          602 g          422 t
ORIGIN      
        1 tggggcgcgt taaaaccggg attggccagt tcatcgacgt ttcgcttcta caggtcgcca
       61 cggcattcat tgaatcgcac gtagcggatt atctaaacgg cggcgaagcg gtctcgcgcg
      121 agaatttccc caaaggccgg atttacagtc tgctcgattg tgacaagaaa ccgttcatgg
      181 ttcacctctc gggccacaat caagcgtggg aaggactgct ccaggcagcg gaactggatt
      241 cgttgagcgg tgatccgcgc tttgcttccc gcaagcagcg caccgagagg cacgaggaga
      301 tcgtcaagat tctccgccag cagtttgccg gtaaaccgcg tgactactgg ttgccgcgat
      361 tggatgccaa cagtattccc aatgcgccga tcaacaccat ccaggaggta ttcgaacatc
      421 cgcagatcaa gcatctgggt attcctcgac agattccgca tccgcaaatg ggcgtgacca
      481 atctggtcgg cagcccgatt aatctatccg gcaccccggc gcagttttac cgaccggcgc
      541 cgctgcttgg cgaacacacc gaagaagtgt tgggacaatt gggatatgac agagcagctc
      601 tcaaggagct gcgtgatgat cgagtaattt aattggttga aatatagtag ggcgttcaaa
      661 aacgtccaga tgcgcggcgc gcgacggacc gtgagcggaa gcgtactctt gaggtacgct
      721 ggaagcgaac gggacgagcg taacaaagca ggtgggcgtt ttgggacgcc ctaaaaagga
      781 gaaatagatg gatttcaatg tatctgccga cccgccccac gtagtcgaat tcagaaaaga
      841 agtcgcggcc tggatggccg aacaatttgc cggccacgag cacctgcgct ggtcggcctc
      901 gtggtcaacg cgcgaggatc aagacgagta taagtttcgc cgcggactgg ccgagaagct
      961 cggcgccaaa ggctggctgt ttccgattta tcctcaaaag tacggcggcg cagacttgac
     1021 catcgaccat cagatggtgc tcgagaccga gctgttcaag tacgggctca atctttcaca
     1081 cgtattctac accctggctc gtatcgtggc gccggtgcta attcgctggg gaaccgaaga
     1141 acagaaaagc gaatttctcg agccgattac gcgagcgcag atctgtgtgt ggcaggtgct
     1201 caccgagccg cacggcggca gcgacatcgc caattgccaa accaaggcga tccgcgatgg
     1261 cgatgattat gtcgtcaacg ggcagaaagt catggtcggc catcatctgc cgccggatta
     1321 tctctggacc ctggtctgca ccacaccggg cggcaagcgc catgaaaacc ttgcctggct
     1381 gcacatcccg gcaaatcttc ccggcatcac gatccagcac atgcatatga tgatggggat
     1441 caagaactct gtattcttcg acaatgtccg cgtgccggcg aaatatctga tcggtggcga
     1501 gaataatggt tggaaagtcg ccagcaccca catggagctt gaacatgggg gcgacggccg
     1561 tatcgctgcg gatccgctga tcgaaagagt ggtcgaatat tgccgcagta cgcacatcga
     1621 cggcaagccg atcatggaag atccacgagt gcgcgatctg atcgccgaaa tgaccatcga
     1681 gttgaatacg ctcaggcgct ttaaccaact catctattgg cgccgcttcg ctaaaaaacc
     1741 gcatccctac ggagggccgc agcaacgcta ttatcaacgc atgttgcgcc tgcgaaacgg
     1801 cgaacggttg caagcaatca tgggggccga tgccctggaa cagaacctgt cggttcatga
     1861 agtcgtcgat ttcgaatacg cggtgcgcac gggtccgggg caattgcacg gcggcgggac
     1921 tttagacacc gaccgcttga tcttggcccg ccgcctcggc ttgggccgtc cgacaaccga
     1981 gaaagcgccg gaaacggatt aaattgaaga taactgcctt aacgttttga acgacttgaa
     2041 cgatttgaac gttttgaacc aatgtcactc cagatcgttc aagcggttca agcagttcaa
     2101 aacg
//
LOCUS       DASUTG010000391         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_505586, whole genome shotgun sequence.
ACCESSION   DASUTG010000391 DASUTG010000000
VERSION     DASUTG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505586"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF089_08965"
     CDS             complement(<1..206)
                     /locus_tag="VF089_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174132.1"
                     /translation="MDNEEVIADKIGSFYGTNTAVSSIIALAGDASSRRYYRVVIKAP
                     GAPKSVVVMELPAGSALPLSSEELA"
     gene            complement(258..476)
                     /locus_tag="VF089_08970"
     CDS             complement(258..476)
                     /locus_tag="VF089_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(435..437),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX6174133.1"
                     /translation="MSSEPVLIFGQDLUPYTSGAREDFSRRKIPFEYINVLEDDAGLA
                     RMLKHSNGRRQIPVIVEAGKVTVGFGGS"
     gene            598..1431
                     /gene="rsmI"
                     /locus_tag="VF089_08975"
     CDS             598..1431
                     /gene="rsmI"
                     /locus_tag="VF089_08975"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6174134.1"
                     /translation="MPGTLYIIATPIGNLEDITLRAIRTLKDVDIIAAEDTRHTQTLL
                     RHFNINTPLTSYHEHNERAKTSQLVARLECGESVALVSDAGTPGISDPGYRLVSEATR
                     LGIRVVPIPGPSALIAALSASGLPTDGFKFSGFLPARKRERRSKLQELRLERYSIVAY
                     ETPHRLKESLDDIREIFGDRRMVMGREITKIHEEFLRGRISEVIAEISRREIRGEVTL
                     IIEGCSEVDPPSEEALRDEIAKLVAEGLRVKEIAEVLGEKYGYSKRHIYGLAMDQGRP
                     R"
     gene            complement(1428..2816)
                     /locus_tag="VF089_08980"
     CDS             complement(1428..2816)
                     /locus_tag="VF089_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174135.1"
                     /translation="MAPDSKLHAATRGRILIVDDDRQMCQLLIDVLAEEGYLVEAVHD
                     GRAALEKFRANGCDLMITDLMMPRMRGTELVKEVKGVDPDAVILLITAFGSIESAVEA
                     MKAGAFHYVTKPFRTDEMILHVSRGLQQRRLCEEVQRLRSEILERNSFENIIGKSEKM
                     REVFSIVRQTSDLAANVLILGESGTGKEMVARAIHHHSWRAQGPFIPLNCAAVPETLL
                     ESELFGYLRGTFTDARKDRRGLFQEASGGILFLDEISEIPINLQAKLLRVIEDKEVRP
                     LGANQSEKVDTRIVSACNVDLEQLIEQGRFRQDLYYRLNVIRIELPPLRERSEDIPLL
                     AEHFLRKFAPQAKREIDSIDQEALAALVNYRWPGNVRELEHTIERAVLLGKERSIGLK
                     DLPLQSQANNGTSVPLAAAVANGYTLRDLEREYILRVIESTYGNKAEAARILGVDRTT
                     LYRKLEEYKLKT"
     gene            complement(2800..>3014)
                     /locus_tag="VF089_08985"
     CDS             complement(2800..>3014)
                     /locus_tag="VF089_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174136.1"
                     /translation="HVFDPFFSTKDVGEGAGLGLAVTYGIIKEHGGEITVESDPGKFT
                     RFLIHLPQGQQKGALVQGNLENGTRL"
BASE COUNT          662 a          848 c          802 g          702 t
ORIGIN      
        1 gctagctctt cgctcgagag cggcagagca ctgcccgcag ggagctccat gacgacgact
       61 gattttggcg cgcctggggc ttttataaca acccggtagt aacgccgact cgaggcgtcc
      121 ccagccagtg cgatgataga agaaacagcc gtgttcgtgc cgtagaatga gccaatctta
      181 tctgcgatga cttcctcgtt atccataatt gaacatttac tcgattttcg tgccggcgcc
      241 gccggccatc aaacctctca gctgccgccg aaacccaccg tgaccttgcc ggcctcgacg
      301 atcacaggta tttggcggcg gccgttcgag tgcttcagca tacgcgccag gccggcatca
      361 tcttcgagca cgttgatgta ttcgaaaggg atttttcgcc gggagaaatc ctcacgggct
      421 cccgaagtat agggtcaaag atcttggccg aaaataagca cgggttcgct gctcatgaat
      481 ttgctccttt cttaggattg ttaaccggat attactatga aaggcggata aattctgcat
      541 gttttcaggg cagcaaattg aaataaactt ggcccgcgtg ttactgggtg cgtagtcatg
      601 ccaggcactc tgtacatcat cgcgacacct atcggtaatc tcgaagacat cacactacgg
      661 gcaatccgaa cactaaagga tgtcgatatc attgcggccg aagacacccg gcacactcaa
      721 actctactga ggcacttcaa catcaacacg cctctgacga gttatcacga gcacaacgaa
      781 cgagccaaaa ccagccaact ggtcgcgcgc ctcgagtgcg gcgaaagtgt tgccctggtc
      841 tcggacgcgg gcacaccggg aatctccgat ccgggctatc gcttggtcag cgaggcgact
      901 cgactgggaa ttcgggtcgt cccgattcct ggtccgtcag cattgatcgc cgctctcagc
      961 gccagcggtc tgcccaccga cggattcaag ttcagtggat tcctaccggc acgaaagcgc
     1021 gagaggcggt ccaagctgca agagcttcgc ctcgagcgct atagcatcgt agcttacgag
     1081 acaccgcatc ggctcaaaga atcgctcgac gacattcggg agatttttgg tgaccgccgg
     1141 atggtgatgg ggcgcgagat caccaagatt cacgaggagt ttcttcgcgg ccgcatcagc
     1201 gaagtgatcg cagaaattag ccggcgagaa attcgtggcg aagtgactct catcatagaa
     1261 ggctgctccg aggtggatcc tccttccgaa gaggcgctgc gcgatgagat tgctaagctc
     1321 gtagccgagg ggttgcgggt aaaagagatt gccgaagttc tcggagagaa gtacggctat
     1381 tctaaacgcc acatctatgg tttagctatg gatcaaggtc ggccacgcta agtcttgagt
     1441 ttgtattctt ctagttttct atagagggtt gttcgatcca cacccagtat ccgcgccgct
     1501 tcggccttat tcccataggt gctctcgatt acgcgtaaaa tatactctct ttccaaatct
     1561 ctgagggtgt atccatttgc cacagcggcg gcaagcggca cagaagttcc attattagcc
     1621 tgcgattgca acggtaaatc cttcaagccg atgctccgct ctttgccaag cagcaccgct
     1681 cgctcaatgg tgtgttccaa ttcgcgcaca tttcccggcc agcgataatt caccagcgca
     1741 gccaacgcct cctggtctat ggagtctatc tctcgtttcg cctgaggcgc aaatttgcgc
     1801 aagaagtgct ctgcgagaag cggaatatct tccgaccgct cgcgtagcgg cggcaattcg
     1861 atgcgaataa cattcagccg atagtagagg tcctgccgga accggccttg ttcgatcaac
     1921 tgttcgagat cgacgttgca ggctgaaaca atgcgtgtgt cgactttttc cgactgatta
     1981 gctcccaacg gcctcacttc cttatcctcg atgacgcgta acagcttcgc ttgcaggttg
     2041 atgggaattt cgctgatttc atcgagaaaa aggatgccgc cgctggcttc ctggaagagt
     2101 ccgcgccggt ccttgcgcgc atcggtgaac gtgccgcgca aatagccaaa gagctcgctc
     2161 tcgagcaggg tctccgggac ggcagcgcag ttgagcggta taaagggacc ttgggcgcgc
     2221 cagctatgat ggtgaatcgc gcgcgccacc atttccttgc cggtgccgct ttcaccgagg
     2281 atgagcacat tggcggcaag gtcgctggtt tgccggacga tcgagaaaac ttcccgcatc
     2341 ttctcgctct tgccgatgat gttctcgaaa ctattgcgct ccagtatctc actacgaagc
     2401 cgctgcactt cttcacagag ccggcgctgc tgtagtccgc ggctcacatg caatatcatt
     2461 tcgtcggtgc gaaatggttt agttacgtag tgaaaggcgc ccgctttcat tgcctcgact
     2521 gcgctttcaa tgctgccgaa agccgtgatc aaaagaatta ccgcgtcggg atcgacccct
     2581 ttgacctctt taaccagttc cgtgccgcgc attcgtggca tcatcaaatc ggtgatcatc
     2641 aagtcgcagc cgtttgcgcg aaacttctcc agcgcggctc ggccgtcatg cactgcttca
     2701 acgagatagc cttcttcggc cagcacatcg atcagcagct ggcacatctg cctgtcatca
     2761 tcgacaatca ggatccggcc gcgcgtagcg gcgtgtagtt tagagtcggg tgccattttc
     2821 cagattgccc tgaaccagcg ctcctttctg ttgtccttga gggagatgga tgaggaagcg
     2881 agtaaacttt ccggggtcgc tttctacggt aatctcgccg ccgtgctctt taatgatgcc
     2941 gtacgttacc gccagcccga gtccggctcc ttcgccaaca tctttggtag aaaaaaaagg
     3001 atcgaataca tgat
//
LOCUS       DASUTG010000392         4384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1543480, whole genome shotgun sequence.
ACCESSION   DASUTG010000392 DASUTG010000000
VERSION     DASUTG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543480"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..918
                     /locus_tag="VF089_08990"
     CDS             115..918
                     /locus_tag="VF089_08990"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX6174137.1"
                     /translation="MTADHDYREELATAEEAVREAGLIVMRLFKGKFDIREKSKNNPV
                     TTADIEANRRIREIITGRFANDGWLSEEDQDSPKRLNSSRIWIIDPIDGTKEFIEGVP
                     QFAVSIGFVVDGRPTVAAVYNPAEDSLYKAAAGKGAYLNDKPISVTERSSLDGALLLV
                     SRSEPQRKFQVFVEHCEIQKVGSIAFRLAKIAGGDGDGTLTFRSIHEWDICAGVLIVL
                     EAGGKVVDGPGAGLMFNQPQAKHRGVVAANASLADGLQRLWVEAMARNT"
     gene            1010..1261
                     /locus_tag="VF089_08995"
     CDS             1010..1261
                     /locus_tag="VF089_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174138.1"
                     /translation="MERVEELRGKVGFGEFLDGLEQTGVAKQKIMTFLKADPDGQGSV
                     QDQVTAEMTTELMKVMGISGQQTPENVKRIRHSVDKDSK"
     gene            1280..1759
                     /locus_tag="VF089_09000"
     CDS             1280..1759
                     /locus_tag="VF089_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6174139.1"
                     /translation="MEITVYLSIVFLVLYLVGLGLHRYSFLRDKRRMRDESRDGARVI
                     SQVEDLSPGSVKKFWIICQKYRVDGFLVNERGNFHAYVNRCRHMPTPLDFVRNQFVSN
                     DGKYLMCYTHGALYEFATGLCVDGPCKGQALYRLPVRVDRGEVLVGCPEGDLSYLKE"
     gene            1767..2129
                     /locus_tag="VF089_09005"
     CDS             1767..2129
                     /locus_tag="VF089_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6174140.1"
                     /translation="MTAAKEKKIGKRKLVGEVGELTHGASKKFTIRSGDRELEALLVN
                     YEGAFFAYRNRCPHVGITLDWVDNQFFSVDGRYLMCANHGAVFEPKTGECIWGPCAGL
                     GLQSVSLEIEGEKIFAKY"
     gene            2175..2519
                     /locus_tag="VF089_09010"
     CDS             2175..2519
                     /locus_tag="VF089_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX6174141.1"
                     /translation="MSDCLFCGIVDGSIKADIVYRDEAVIAFKDIVPRAPVHILIIPI
                     AHIPMLSNLTPDDANIVATVFAAARKLAEQFGIAKSGYRIVVNCGADAGQTVFHLHYH
                     LLGGRQMGWPPG"
     gene            2568..3827
                     /locus_tag="VF089_09015"
     CDS             2568..3827
                     /locus_tag="VF089_09015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6174142.1"
                     /translation="MAITEWRNETSDMAVHQFDVAAAKLGLDPNLAVRLRRPDRAMVI
                     SVPTRMDDGRVNVFTGYRVQHNDVLGPFKGGLRYHPSVNLGEVSALAMWMTWKCSLVG
                     LPLGGAKGGIACDPAELSRNELQSMTRRYTAEILNFIGPEIDVPAPDMGTNEQIMAWI
                     MDTYSQHKGHAVPEIVTGKPIAIGGTLGRREATGRGVVYTIIEAAKHLRIDLTKCTAA
                     VQGFGNVGSVVVKELNDLGVRIIGVADRTGGFYDVNGLPIARLLETAYQNHSLEGFPH
                     GEKITNAELLELKCDILVPAALEMQITQENAQRLQCRILAEGANGPTTPEADAILRQK
                     DIFVIPDILANAGGVVVSYFEWVQGLQNFFWTEEEITRKLRDILVKAFHEVLQMSQKQ
                     GVEMRLAALMIGIDRVARAMLWRGLYA"
     gene            3906..>4384
                     /locus_tag="VF089_09020"
     CDS             3906..>4384
                     /locus_tag="VF089_09020"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX6174143.1"
                     /translation="MIQATQLRPGMIIVHEKDLYRVTAVHHLTPGNKRGFMQTKMKSL
                     RTGVGTEYKFRSEDRVDQAILQTRQMQYLYAEGDMHTFMDTENYEQMTISADDIGHLL
                     SFLLPNHVVEVELYDGKPVGISPPSTVDLEVVDTEPSMKGATASASYKPAKLETGVT"
BASE COUNT         1080 a         1054 c         1255 g          995 t
ORIGIN      
        1 acagttttcg tctttgaatg aaattgttat cactctgagc aatcgatgct cggttcctat
       61 cggtttggca gggtgtcccg tcccgaaaac cgatttaggg ctaaaggtgg tttagtgaca
      121 gctgatcatg attatagaga agagttggca actgcagaag aagcggtccg cgaagcaggc
      181 ttgattgtga tgcggctgtt caagggaaaa tttgatattc gtgaaaagag taaaaataat
      241 cccgtcacca ccgcggatat cgaagcaaac cgcagaattc gcgaaattat cacgggcagg
      301 tttgcaaacg acggatggct ttccgaggaa gatcaggaca gcccgaaacg gttgaactcc
      361 tcgcgaatct ggatcattga tccgattgac ggaactaaag aatttattga aggcgtccca
      421 cagtttgcgg tgtcaatcgg cttcgtggtt gatgggcggc ccacggtcgc ggctgtttac
      481 aatccggccg aggattcgct ctacaaggcg gcggcaggca aaggagccta cctgaacgat
      541 aagccgatta gcgttactga acgcagttct ttggacggcg cgttgctcct ggtcagccgc
      601 tccgagccgc aacggaaatt tcaggtattc gtcgagcatt gcgagatcca gaaagttggt
      661 agcattgcct ttagattagc caaaatagcc gggggggacg gcgatggcac actaaccttc
      721 cgttcgattc acgagtggga catctgcgca ggcgtgttga tagtgctcga ggccggcggc
      781 aaggtggttg acggccccgg cgctggtttg atgtttaacc agccgcaagc caaacatcga
      841 ggcgtagttg ccgctaatgc atctttagcc gacggactgc agcgtttgtg ggtcgaagct
      901 atggcgcgaa acacgtaact tgggcttcga atcgtttatg cgtcgatgtg tttactgtgg
      961 cgaccgttcg agcttgtggt cgcggatgtg tccagattgc cagaagctca tggaacgagt
     1021 cgaagagttg cgtggcaaag tcggctttgg cgagtttctc gatgggcttg agcagactgg
     1081 agtggccaag cagaagatca tgaccttctt gaaagccgac ccggacggac aaggaagcgt
     1141 ccaagatcag gtcactgccg aaatgacaac tgagttgatg aaggtcatgg gaatatcagg
     1201 gcagcagacg cctgagaacg tcaagcggat ccgccattcg gtcgacaagg attcgaaata
     1261 gctctcctct tagcctgtcg tggaaattac cgtctacctg tcaattgttt ttctagttct
     1321 ttacctggtg gggttgggcc tacaccgata cagctttttg cgcgacaaac ggcgcatgcg
     1381 cgacgaaagc cgcgatggcg ccagagtcat cagccaggtc gaggatctga gtcccggttc
     1441 ggtcaagaag ttttggatca tctgccagaa gtaccgggtc gatgggtttt tagtgaacga
     1501 acgaggcaac tttcatgctt atgtcaaccg atgccggcat atgccgacgc ctcttgattt
     1561 tgttcgtaac cagtttgtca gcaatgacgg caaatacttg atgtgctata cccatggcgc
     1621 tctttatgaa tttgccaccg ggttgtgtgt tgatggaccc tgtaagggcc aagccctcta
     1681 ccgcttgcct gtcagagtag accggggcga ggttttggtc ggatgcccgg aaggagacct
     1741 gtcctacctc aaggagtgaa gccagcatga ctgcagccaa agaaaaaaag atcggtaagc
     1801 gaaaactcgt cggcgaagtg ggtgaactca cccatggcgc cagtaaaaaa tttaccatcc
     1861 gttccggaga tcgtgagttg gaagcattac tcgtcaacta cgagggggcc tttttcgcct
     1921 atagaaatcg ttgccctcat gtcggaatca cactggattg ggtggataac cagttcttct
     1981 cggtggatgg tcgttacttg atgtgcgcca atcatggtgc cgtgttcgag cccaagaccg
     2041 gcgaatgtat ttggggccca tgcgccggtc tggggctcca gagtgtctcg ctggaaatcg
     2101 aaggcgagaa aatttttgcc aagtactaga cgccggagat tgattcgcag cgggataaaa
     2161 atcggagaac cagtatgagt gattgcctgt tctgcgggat cgtcgatggc agcatcaaag
     2221 cggacatcgt ctaccgcgat gaagccgtga ttgccttcaa ggacatcgtg cccagagccc
     2281 cggttcatat tctgatcata ccgattgcgc atatcccgat gctttcgaat ttaacgccgg
     2341 atgacgctaa catagtcgct acagtctttg ccgctgccag gaaactggcg gagcagttcg
     2401 gaatcgcaaa aagcgggtat cgcatcgtcg tcaattgcgg cgccgacgcc ggtcagaccg
     2461 tttttcacct gcattaccac ctgctcggcg gccgtcagat gggttggcct ccgggctgac
     2521 gtgaatcgct gttaatacag aaaacattat ggagctaatg gagcactatg gcgattactg
     2581 aatggcgcaa cgaaaccagc gacatggcgg tgcaccaatt cgatgtcgcc gctgccaagc
     2641 tggggcttga tccgaatctt gctgttcgct tgcgcaggcc ggatcgggcc atggtcatca
     2701 gcgtgcccac ccgaatggat gatgggaggg tcaatgtttt caccggctat cgcgttcagc
     2761 acaatgatgt tctgggaccg ttcaaaggag gacttcgtta tcatcctagt gtaaacttgg
     2821 gcgaagtctc cgctctggcg atgtggatga cttggaaatg ctcgctggtg ggcctgcccc
     2881 tcggtggcgc aaaaggcggt atcgcgtgcg accccgccga gttgtcacgc aatgagcttc
     2941 agtccatgac caggcgttac actgctgaaa ttctaaactt catcggccct gagatcgacg
     3001 taccggcccc ggatatggga accaacgagc aaatcatggc gtggattatg gacacttact
     3061 cgcagcataa aggccacgca gttcccgaga tagtaacagg gaagccgatc gctatcgggg
     3121 gcaccttggg gcggagagaa gcaacgggtc ggggtgttgt ctacacgatc atcgaagccg
     3181 caaagcatct gagaatagat ctcacaaaat gtaccgcagc agtccaggga tttggcaatg
     3241 tcggatcggt cgttgttaag gaactcaatg acctcggggt gaggataatc ggcgtggcag
     3301 atcggacggg cggcttttac gacgtcaatg gtctgccgat agccagactg ctcgagactg
     3361 cctatcaaaa tcatagcctt gaaggttttc cccacggaga gaaaattacc aatgcggaac
     3421 tcctggagct taaatgcgat attctagtcc ccgctgcatt ggaaatgcag atcacccaag
     3481 aaaatgctca acgtttgcag tgccgcatcc tggcagaggg agccaatggc cccacgacgc
     3541 cggaagctga cgcaatactg cggcagaagg acatcttcgt tatcccagac atcttggcca
     3601 atgcgggcgg tgtggtagtt tcgtactttg aatgggtgca gggcttacaa aatttcttct
     3661 ggactgaaga ggagattacc agaaagttgc gcgatatttt ggtcaaagca ttccacgaag
     3721 tcctgcagat gagtcagaaa caaggcgtcg aaatgcggct agccgctttg atgatcggca
     3781 ttgatcgcgt tgcacgcgcc atgctgtggc gcggattata tgcatgatta gaggtcgaac
     3841 taaacaatcg atgaagcgca gcaccccggt aacaacagcg gcggtggcga aggaaggaag
     3901 ttaacatgat acaggcaacc caactaagac ccggcatgat tattgttcat gaaaaggatc
     3961 tatatcgtgt caccgcggtg caccatctaa cccccggaaa caagcgggga ttcatgcaga
     4021 ctaaaatgaa aagtctacgt acgggtgtcg gtaccgaata caagtttcgc tctgaagatc
     4081 gtgtcgatca ggcgatttta caaacgcggc agatgcaata cttatatgct gaaggagata
     4141 tgcacacctt catggacact gagaactatg agcagatgac aatatccgcc gacgacatcg
     4201 gtcatctact atcgttcttg ctgcccaatc atgtggtgga agtggaattg tacgacggaa
     4261 aaccggtggg tatcagcccg ccttcaacgg tggatctgga ggtcgtcgat accgaaccct
     4321 cgatgaaagg agctacggca agcgcttcct acaaaccggc gaaactcgaa actggagtta
     4381 cgat
//
LOCUS       DASUTG010000393         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1841748, whole genome shotgun sequence.
ACCESSION   DASUTG010000393 DASUTG010000000
VERSION     DASUTG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841748"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF089_09025"
     CDS             <1..495
                     /locus_tag="VF089_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6174144.1"
                     /translation="VGGLTLDPVTKTPIVILKDTDNKLNLPIWIGLLEATAMATEIEG
                     IKMARPMTHDLIKNILGEVGCAVDSVEITDLKENTYFALVYLNISGRQVVIDSRPSDA
                     IALALRTKSPIFVAKAVLEASSILQQNEEGKEPPVENVSNVSQEKWAEILEKMTPDDF
                     KYKQ"
     gene            551..1246
                     /locus_tag="VF089_09030"
     CDS             551..1246
                     /locus_tag="VF089_09030"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174145.1"
                     /translation="MATSAKHFSRKELRQPDNFVLFTQRVLGFVQQNRRVLILAGAVL
                     TAVIIGISIWQVYKSRQNEQAAQHFATALGLFRENKHNEAIPEFEKVEAYRWSHYAAL
                     AHLYEANSHLATNDPDQAAAAAQRFIAATDQNSVYRQIGLMTLGHIQELKNQCSEAIQ
                     RYTEAERIAGVLRESAVLGKARCYTTIGDNKSSIAAYQQYIKENPNSPIAARLMLQVA
                     ELQTKTETEPAVK"
     gene            complement(1718..>2074)
                     /locus_tag="VF089_09035"
     CDS             complement(1718..>2074)
                     /locus_tag="VF089_09035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6174146.1"
                     /translation="KIDIHYEFVVRSIDPVKKQIDGLYNYSLPYDLLVMVPPHRPAQV
                     LLDCGLAEAETGIRVDYDTLQTKWDNVFAIGDCADMPASKAGGVAHQEADVLAHNLAV
                     EITRKGKPLDLWLHTI"
BASE COUNT          535 a          538 c          500 g          501 t
ORIGIN      
        1 gtgggtgggc tgaccctcga cccagtcact aaaactccca ttgtcatctt aaaagacacc
       61 gacaacaaat tgaatttgcc catctggatc ggtttactag aggccacggc gatggccacg
      121 gaaatcgaag gaatcaaaat ggctcggcca atgacccatg atctaatcaa gaatattctc
      181 ggtgaagttg gttgcgctgt ggactctgtc gaaatcactg atctcaagga aaatacctat
      241 tttgcgctgg tgtacttaaa catttcaggt cgtcaggttg tgatcgactc ccgtcccagc
      301 gacgcgattg cgttggctct gcgcaccaag agcccaattt tcgtcgccaa ggcagttctc
      361 gaagcctcca gcattcttca acagaacgag gaagggaagg agcctcccgt cgaaaacgtc
      421 tccaacgtgt ctcaagaaaa gtgggcagag atcctagaga agatgacgcc cgatgatttc
      481 aaatacaagc agtagcggtt cttaaacttc ccttaaactc gacgtttcca cttttagcga
      541 attagaagct atggcgacta gcgctaagca cttctcccgt aaggaactcc gtcaacccga
      601 taactttgtc ttgttcaccc agcgagtgtt gggtttcgtt caacagaatc gtcgggttct
      661 tatcctcgct ggcgctgtgc tcacggctgt gatcataggg atttcgattt ggcaggttta
      721 taaaagccgg caaaacgagc aggcagcgca gcatttcgct acggcgctcg ggctttttcg
      781 cgagaacaag cacaacgaag ccattcctga gtttgaaaaa gttgaagcat accgctggtc
      841 tcattatgcg gctctggccc atctttatga ggccaacagc cacttagcaa caaacgatcc
      901 ggaccaagcg gccgcggcgg cccagcgctt catcgctgcg accgatcaga actccgtcta
      961 tcgacagatt ggtttgatga cgcttggaca catacaagag ctgaaaaacc aatgctccga
     1021 agcaatccag cgctataccg aagctgaacg gatcgcggga gttttaagag aatccgccgt
     1081 gctcggaaag gcacggtgtt atacgaccat cggtgataac aaatcatcta ttgccgcata
     1141 tcagcagtac atcaaagaga accctaactc accaattgcg gcacgtctca tgctgcaagt
     1201 tgccgaactg caaaccaaga cagagaccga gccagcggtt aaatagttgg tacggtcata
     1261 tcggcatacg gtcttaatac aagccttttc atccattcga atctgacgac ctgaactcgg
     1321 ttcttgatcc aaacgaacgg gtccaattct attgtgctgc tgttctgacg cttatagcta
     1381 acgtctgata agatatatta tgtcaacttt tgggtatgcg gcctgaacct atacacaacg
     1441 gatgttctaa ggcggtctag tccacccccg cttccctggt aaactccggc tgatgattgg
     1501 cagcagtcga ttgaccgacg ctcaatgctt tcgacaattc tagatgagat agactttaga
     1561 agtaccgcca taaccaccat ttttcaaagg caattttggc ccaatagatc agacgatttg
     1621 gtccccatgt ccgcggaaac gtgatgccgt aaggcggcca tccggtagga taaatcgttc
     1681 tgtttgcagc ggcgcgtcca gcgccgaatg cgagtagtca tattgtgtgg agccagaggt
     1741 ccagcggctt gccctttcga gtgatttcga cagctaaatt gtgcgccagc acgtcagctt
     1801 cctggtgggc aacacctccc gcttttgacg ctggcatatc ggcgcaatct ccgatcgcaa
     1861 aaacattgtc ccatttggtc tgcagcgtat cgtagtctac tcgtatccca gtttcggcct
     1921 ccgccaatcc gcaatcgagg agaacttgcg ctggacgatg cggcggaacc atcaccagaa
     1981 gatcgtaggg caaactatag ttatagagtc catcaatttg ttttttgacc ggatcgatag
     2041 agcgcacgac aaattcgtag tggatatcaa tttt
//
LOCUS       DASUTG010000394         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1396721, whole genome shotgun sequence.
ACCESSION   DASUTG010000394 DASUTG010000000
VERSION     DASUTG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396721"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VF089_09040"
     CDS             complement(<1..1120)
                     /locus_tag="VF089_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX6174147.1"
                     /translation="MATKPRQRSFTAGLLDTIERWGNKLPHPFNLFLLFSLIVLIFSS
                     LAHALGLAVVHPLTGKQIAAVNLLDRQGIQNIITKAVGNFTGFAPLGTVLVAMIGVGV
                     AERSGLFSAALRGFVRSVPSWLITAALVFAGVNASLTVDAGYVILVPLGAVLFAQVGR
                     HPIAGLAAAFAGVSGGFSANLFLTPLDPLLAGLTQEAARLYDPQYVVPPSANYYLMIG
                     STFLLTVLGTMVTNWVIEPRLGPYRIQGEAGAAALENKEKRGLRAAGLTLFAELIVLL
                     FLVAPNDALLRDAKGTLEPFFQGMVVLMALGFLLPALVYGLAAGTIASNHDVARMIEE
                     TLGTMGSYIALAFAASQFVAYFGWSNLGLILAVSGADLL"
     gene            1439..>2220
                     /locus_tag="VF089_09045"
     CDS             1439..>2220
                     /locus_tag="VF089_09045"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6174148.1"
                     /translation="MAKYRVTVDTGGTFSDFVFFNEETGEISITKLPSTPREPFQAVL
                     NGVKELLERGVAAGDVSFFCHGTTVGTNALLEEKGAKTGLLVSQGFRGIYEVMEQTRG
                     YGPATYDLFFEKPRLLAPPFYTEEIPERVDFRGNVLKPIDVEAARLAIRRLKKKGVQS
                     VAVCFLFSFLNPDHELKIKELFGEEFPEAHLSLSYEVLPQIREFYRMSTTIINAYIQP
                     VMANYLGSLENRMREMAITTPKLYIMQSNGGVSTFAGSAKKPV"
BASE COUNT          576 a          590 c          602 g          452 t
ORIGIN      
        1 gcaagagatc cgcgccgctg acggccagta tcaacccgag attggaccag ccaaagtaag
       61 cgacaaactg gctggccgcg aacgccagcg cgatatagct ccccatcgtt ccaagcgtct
      121 cttcgatcat gcgtgccaca tcgtgattgc tggcgatggt tcctgccgcc aagccgtaaa
      181 ccagcgccgg aagcaagaat ccaagcgcca tcaaaaccac catcccttga aagaatggct
      241 cgagagtacc cttggcatca cgcaagagtg cgtcattcgg cgctactagg aacagtagta
      301 cgataagctc ggcgaaaaga gtgagacccg cagctctcaa gccgcgcttt tccttgtttt
      361 ccagggctgc tgcgccggct tccccctgga tccgatatgg gcccaatctt ggttcaataa
      421 cccagttggt taccatcgtg ccgaggacag tcagtaagaa agtggagccg atcatcaaat
      481 agtaatttgc actgggcgga accacgtact gcggatcgta aagcctcgct gcctcctggg
      541 tcaggccagc taggagcgga tcgaggggcg tgagaaacag gttggcgctg aaacctcccg
      601 atactccggc gaaggcggcg gccaatccgg cgatgggatg acggccgact tgcgcaaaca
      661 ggaccgcgcc gaggggtact aagatgacat atcccgcgtc aacggtgagg ctggcgttga
      721 caccggcaaa gaccaacgct gcggtaatca gccaagacgg cacacttctg acaaatcctc
      781 tgagagccgc cgaaaaaagg ccgctacgct cagccacacc aacaccgatc atcgcgacca
      841 gaaccgtgcc gagcggagca aagccggtaa aattgcccac agccttggtg atgatgttct
      901 ggataccctg acgatcgagt aggttaacgg cggcaatttg tttcccggtc agcggatgga
      961 ccactgctaa tcccagagca tgggccaggg atgaaaaaat caaaactata agcgaaaaga
     1021 ggagaaacaa gttgaacgga tgtggcagct tgttacccca gcgctcgatc gtatcgagta
     1081 agcctgcagt aaagcttctc tgtcgcggtt tagtcgccat cgccttgaat aatactgtcg
     1141 gattaaatca gtcaagtaga cagatctgtg ccttacccag tagcacaacc cgaacggaag
     1201 cagaaaagct cagcgaaaga aactaacttg atgataagat cgtgtacacg gtacccgtta
     1261 aatcgaacac ttcatgcgtc attcaggttg ccgcgcgcac ttgttgggca ggtcgtaatc
     1321 acctggagct ttaaggcccg ttatgggtaa atcaacgctt cacgtcgagg aaaagagcgt
     1381 gttcagcatt gccaaagctg attgaagacc ccggggataa gtgttagacg aaacagctat
     1441 ggcaaagtac cgagtaaccg ttgacacggg aggaacattt tccgactttg tctttttcaa
     1501 tgaagagacc ggtgagatca gcatcacgaa attgccatca acgccgaggg aaccatttca
     1561 agcggtgtta aacggcgtca aggaattact cgagcgcggg gtggcggctg gagatgtttc
     1621 gtttttttgc cacggcacca cggtgggcac caatgcgctc ctggaagaaa aaggcgccaa
     1681 gacgggcttg ctcgtaagcc agggctttcg cggcatttat gaagtcatgg agcaaacccg
     1741 aggctacggc cctgctacct atgatttgtt ttttgaaaag ccgcgactcc tggcgccacc
     1801 cttttatacc gaagagattc ccgaacgggt cgactttcgc ggcaacgtgt tgaagcctat
     1861 cgacgtcgaa gccgcgcgcc tagcgatcag gcgcttgaag aaaaaaggcg tgcagtcagt
     1921 ggcggtgtgt tttttgtttt catttttaaa tccggaccac gagctcaaga tcaaagaact
     1981 cttcggcgaa gaatttcccg aggcgcacct gtcgctttcg tacgaagtct tgccgcagat
     2041 tcgcgagttc tatcgaatga gcacgaccat tatcaacgca tatatccagc cggtgatggc
     2101 caactatttg ggcagccttg agaaccgtat gcgcgaaatg gcgatcacca caccgaagct
     2161 ctatatcatg caatccaacg gtggcgtttc gacgtttgca ggctcagcca aaaagccggt
//
LOCUS       DASUTG010000395         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1988342, whole genome shotgun sequence.
ACCESSION   DASUTG010000395 DASUTG010000000
VERSION     DASUTG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988342"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VF089_09050"
     CDS             <1..890
                     /locus_tag="VF089_09050"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174149.1"
                     /translation="GWWFLSALLRGGTTSIANHQPGRIKMSLSSLASTLSLALGLCLI
                     TAIGFAQPAQQYEPQVGQEGKDVVWVPTPQALVDKMLDMAKVTPKDFVIDLGSGDGRT
                     VITAAKRGAKAHGIEYNPDMVELAKRNAAKEGVSDRATFVKADLFESDFSQAQVITMF
                     LLPSINLKLRSKILDLKPGTRVVSNSFDMEEWKPDETANAEGCSNWCTAYLWIVPAKV
                     NGTWKLPNGELTLKQTFQMISGTLKSGNVVTPVKGKLNGEQITFTAGEGQYSGRVNGN
                     SMEGSLKSGGKWSATRAGQ"
     gene            complement(1174..1560)
                     /locus_tag="VF089_09055"
     CDS             complement(1174..1560)
                     /locus_tag="VF089_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174150.1"
                     /translation="MSVNLEAAVMENINELKMTISEKSLEVAELEKELKRYESVLALL
                     RDDHNRNATRKGRPRGKTDLVEVLGGLPETFTSKEFIKAATRTKKPSVYLRLALSRWA
                     KEGRVKRLERGKYQKVRNGSSHRLAA"
     gene            complement(1840..2865)
                     /locus_tag="VF089_09060"
     CDS             complement(1840..2865)
                     /locus_tag="VF089_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX6174151.1"
                     /translation="MGKFHVVYFGSNDVPVDLIKPILSEIDADMTIRRPTNDAEVVEM
                     GKDADGIIMHGSVPLSREIISQLKRCKVVCRTGVGVDRMDLKAASDHGMIICNAAGCN
                     SIEVSEQTIGLLIAASRKLVRMNQYVHDGKWRRHTAELHAYRGRVHRIQGRTLGIVGL
                     GHVGRQVAPRAQGMRLNVQAVDPYLDSKIAQSLGVKLVSFDELVATSDFISLHAPLTR
                     QTRKMFSVPQFKAMKKTAYLINCARGGLVDTEGLYESLVAGELAGAALDVTEPEPLPA
                     DHKILTLPNVIVTCHTAANSDESYIDCQTHAAREVVNVLSGRGPTTEVKDPWLLAEAQ
                     DEGFGGV"
     gene            complement(3015..>3744)
                     /locus_tag="VF089_09065"
     CDS             complement(3015..>3744)
                     /locus_tag="VF089_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174152.1"
                     /translation="VYLPDVDEAVRELRRSVTELGFKGVKLMGGHRNCDLDAEVLWPF
                     YEEVCKLDVPILVHPGTRIFEAQDCHPWLVGSERYDGFKMLATALGFPLTYMVSAARL
                     IMSGTLDRFPNLRFAFFEGGIGWVPWLMKTLNHHTLAEAPETTSVNDFFKGRDRLKKE
                     PSAYFEQLYVAAIAWEDYLPKIVEIWPDHNIIIGSDFDHGDAISTWPRTVAPIRAMSG
                     LSDRDKDKILGGNAMKLFGMKAAH"
BASE COUNT          890 a         1050 c          955 g          849 t
ORIGIN      
        1 atggatggtg gtttttgtct gctctattga ggggcggtac gacaagtatc gccaatcatc
       61 agccggggag gattaaaatg agtttatcga gcttagctag cactttatca ttggcgctgg
      121 gcctgtgtct gattaccgca attggatttg cccagccggc acagcaatac gaaccccagg
      181 tcggccaaga aggcaaggac gtggtctggg tgccaacgcc tcaagctctg gtcgataaga
      241 tgctcgatat ggcaaaagtc acccccaaag acttcgtcat cgatctgggt tcgggtgatg
      301 gccgcactgt catcacggcg gctaaacgcg gcgccaaagc gcacggcatc gaatacaatc
      361 cggacatggt cgagctcgcc aagcgcaatg ccgcaaaaga aggcgtcagt gatcgagcta
      421 cgttcgttaa agccgactta ttcgaaagcg atttctcgca agctcaggtg atcaccatgt
      481 tcctcctgcc cagtatcaat cttaaacttc gctccaagat cctcgattta aaacccggga
      541 cgcgcgttgt atcgaattct tttgatatgg aagaatggaa gcccgacgaa acggccaacg
      601 cggaaggatg cagcaactgg tgcaccgcct acctgtggat cgtaccggcg aaagtaaacg
      661 gcacgtggaa gttgcccaac ggggagttga cgctgaaaca aacctttcaa atgatcagcg
      721 gcactctaaa atccgggaac gtcgtcacac cggtcaaagg caagctaaac ggcgagcaga
      781 ttactttcac tgccggtgag ggacaataca gcgggcgggt aaacggcaac tccatggagg
      841 gatcgctaaa atccggcggc aaatggagcg ccacccgcgc ggggcaataa agaacactaa
      901 tcagccgtaa atggttgata cgatcggctt gcttacgagt tgcggaagcc ttagacccgc
      961 ggccggtcag cagttaattc acacaatgcc ggttccgcca cactgcaatc caccccaccg
     1021 gcgagagcac ctcacaccca taggccgacg ggaaagcctc ccgctaaaat ttattctaag
     1081 ctcgcgttca gagagacggt gaggctccgg tcaatgaccg gagccttatg ctacaatgcg
     1141 tattggaaat gatcacacaa cgatgtgatg cgattaggct gctagccgat gcgagctacc
     1201 atttctaacc ttctggtact tacctctttc cagtcgcttg actcttcctt ccttcgccca
     1261 tctggagagc gccagccgga gataaaccga tggttttttg gtgcgcgtcg ccgccttgat
     1321 gaattccttg ctggtaaatg tttcgggcag cccacctagc acctcgacga gatctgtctt
     1381 tccgcgcggt cgaccttttc ttgtcgcatt cctgttgtga tcatcacgca gcagagccag
     1441 gacggactca tagcgcttca actctttctc caactcggct acctcaagac tcttttcaga
     1501 gatggtcatc ttgagttcat tgatattttc cataaccgcg gcttcgagat ttacggacat
     1561 aacacgatac ctcctcgctg aagagtatgt aatctggcag atcaactcaa gatggctcga
     1621 tataaatctc ttttgtttgt aagtattttc tgagatggat cggccgtagc atcgcatctc
     1681 acaaagctat agccggaagg tacaaacgga aagatggcgc acgccagatc acctcgcctg
     1741 aaaacaatac tagcacgcca gagacagcaa aggtgcggtt catgcgccgg ggccttcggt
     1801 gcatcaactc acatttgcaa ccggattacc tgctcgacgt tacacgccgc cgaatccttc
     1861 atcctgcgct tcagccaata accagggatc tttgacttcg gtagtggggc ctcggccgct
     1921 aagaacattg acaacttcgc gcgccgcatg tgtctgacaa tcgatgtagg attcatcgga
     1981 gttggcggcg gtgtgacatg ttacgattac atttggcaag gtaagaatct tgtggtccgc
     2041 cggcagcggt tccggctcgg taacgtcaag ggcggctccg gccaattctc cagccacaag
     2101 ggattcgtac aaaccctcag tgtcgaccag accgccgcgc gcgcaattga tcaggtaagc
     2161 ggtttttttc atggctttga actggggcac gctaaacatc ttgcgggtct gtctagtgag
     2221 aggggcgtga aggctgatga agtctgatgt tgcaaccagc tcgtcaaacg acaccagctt
     2281 gacaccgagt gactgcgcaa tcttcgaatc cagatacgga tcgaccgctt gtacattcag
     2341 cctcattcct tgggcgcggg gcgccacttg ccggccgaca tggccgagcc cgacgatgcc
     2401 cagcgtgcgt ccctgaatgc gatgaacccg gccgcgatag gcatgcagct ctgcagtatg
     2461 ccgcctccat ttaccgtcat gcacgtactg attcatgcgc acgagctttc tcgaagcagc
     2521 gatcagcagt ccgatggttt gttcagatac ctcaatcgaa ttgcaacccg ccgcattgca
     2581 gataatcatg ccgtgatcgg aagctgcttt aagatccatc cgatcgactc cgacaccggt
     2641 acggcagaca actttgcaac gcttcagttg cgagattatc tcacgcgata gcggcaccga
     2701 cccgtgcata atgatgccat cggcatcctt gcccatttca actacctcgg catcgttggt
     2761 cggccgtctg atcgtcatat cggcgtcgat ttccgacagg atcggcttga tcagatccac
     2821 cggcacatca ttgctgccaa aatacaccac atggaacttg cccatcttaa acctccattt
     2881 cactcagcaa atcggatgac cgatagtatt ccgccgcgct tcagcggtcg tgcgaagcca
     2941 gacgaattga ttacgggcga cgtactcgcg actcgagtga cgctacgatc gtctaagtct
     3001 ttttcgccgg gctgttagtg cgccgctttc atgccgaaca gcttcatggc gttgccgccg
     3061 agaattttat ctttgtctct atcgctcaaa ccgctcatgg ctctgatcgg cgcgaccgtc
     3121 ctaggccagg tcgagatcgc gtcgccgtga tcgaaatcgc tgccgatgat gatgttgtga
     3181 tcgggccaga tttcgacgat cttcggcagg taatcttccc aggctatggc ggcgacataa
     3241 agctgctcga agtacgcgct tggttctttt ttcaagcgat ctctgccttt gaagaaatcg
     3301 ttaaccgatg tcgtttcggg agcctcggcc aacgtgtgat gattcagagt cttcatcaac
     3361 cagggcaccc aaccaatgcc gccttcgaag aaagcaaacc gcaaatttgg aaatctatcc
     3421 agcgtaccgc tcatgataag ccgagccgcg ctcaccatat aagtgagcgg gaagccgagc
     3481 gcggtggcga gcattttgaa accgtcatag cgttcgctac cgaccaacca cgggtgacaa
     3541 tcctgagctt cgaagatgcg cgttccggga tgaaccagaa tcggcacatc cagcttgcaa
     3601 acctcttcat agaaaggcca aagcacctcg gcatcgagat cgcagtttcg atggccgccc
     3661 atcaatttta cgcctttgaa gccaagttct gtcaccgacc gcctcagttc acgaacagct
     3721 tcatcgacat caggcaaata cacc
//
LOCUS       DASUTG010000396         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1543701, whole genome shotgun sequence.
ACCESSION   DASUTG010000396 DASUTG010000000
VERSION     DASUTG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543701"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VF089_09070"
     CDS             complement(<1..652)
                     /locus_tag="VF089_09070"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6174153.1"
                     /translation="MKIRKAVIPVAGLGTRFLPATKTVPKELLPIIDIPSIQYVVQEA
                     VDAGIQEIIFVTGRGKDGIEDHFDEAPELEQILADRGQTATVDMLRRIAEMTEVVSVR
                     QKKPLGLGHAVLCARDLVGDEPFAVMLADDLIDAETPGIRQLVDIFTETEESVVALMK
                     VPLDEVHQYGVIKGKEVKPHLYAVEATVEKPVAKDAPSQLAIIGRYVLRPEIFPILQ"
     gene            complement(672..1034)
                     /gene="dksA"
                     /locus_tag="VF089_09075"
     CDS             complement(672..1034)
                     /gene="dksA"
                     /locus_tag="VF089_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX6174154.1"
                     /translation="MNKKEVEFFRDLLNQRVQELRSEAGKTVDDMDEDENFPDPSDRA
                     SMESNRNSILRIRDRERKLIFKIQEALQRLEAGEFGICEECGEKIGIERLKARPVTTL
                     CIDCKANQEIAERKAKRM"
     gene            complement(1116..1934)
                     /locus_tag="VF089_09080"
     CDS             complement(1116..1934)
                     /locus_tag="VF089_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6174155.1"
                     /translation="MKRRGFVALLIALIFDPLRSVALAICNPALVRLQQKPRRQLVQV
                     RPGKSPQLGHYFIQWYGHSSFLIHSGSQTKVVADPNFNVTPGIQADAVTVSNDHFTHN
                     NTGAVTGDPVILRGITFRQTWNPIRTSVKDITIINIPSQRGQSWGGAANSIFIYEMGS
                     LCLAHLGNIGHLLTAEQEQVLQRVDVMMVPIDAMTNLGFDDILKVIEQVKPPIVIPMH
                     FDVIRQGELFASFAQEHYPVKRFPGSQLVLSRAMLPKSTEVFVLAHPRAVSLGE"
     gene            complement(2082..2657)
                     /locus_tag="VF089_09085"
     CDS             complement(2082..2657)
                     /locus_tag="VF089_09085"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX6174156.1"
                     /translation="MRKVSIDDIVGLAGYEKIRQDFRRRIIELKQKRRISLGDRVSLV
                     FENRDTVIFQIQEMLRVERITDLDKVRQEIATYNHLIPDSRELSATLFLEIEDQTHLR
                     EELMKFQGIEEGISLRMAGHRLAACFEAGRSKEDKISAVQYVRFRFDDQALETFVSGA
                     KAELIIDHRNYQAAVVLQPETQKLLAEDLTA"
     gene            complement(2730..3155)
                     /locus_tag="VF089_09090"
     CDS             complement(2730..3155)
                     /locus_tag="VF089_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX6174157.1"
                     /translation="MGKSLAGTKSQQNLKEAFAGEAQANRRYLYFARIADIEGYPDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPCTGVPIGNTEKNLKSAIEGETYEYTEMYPGF
                     AKTARDEGLFELAEWFETLAKAEKSHAGRFNKGLDGLGS"
     gene            3396..3716
                     /gene="metG"
                     /locus_tag="VF089_09095"
     CDS             3396..3716
                     /gene="metG"
                     /locus_tag="VF089_09095"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX6174158.1"
                     /translation="MISIDAFRQIELKVATVKSAEPHPNADKLMVLQIDLGTEQRQIC
                     AGIRNQYTPEELVGRQIVVVANLETAKLRGLESQGMLLAASDGERVIFLTPEKSVQSG
                     SKVS"
BASE COUNT          856 a         1049 c          969 g          918 t
ORIGIN      
        1 tctgcagaat aggaaaaatc tccgggcgga gcacgtaacg gccgatgatc gccaactggg
       61 aaggcgcgtc cttggcgaca ggtttttcca ccgtcgcttc gaccgcgtac aggtgaggct
      121 tcacttcttt acccttgatg acgccgtatt gatgaacttc atcgagcgga actttcatca
      181 gggcaacaac cgactcctcg gtttcggtga aaatatccac cagttgccgg atgccggggg
      241 tctcggcatc gataaggtca tcggccagca tgacagcaaa cggctcgtct ccgaccaggt
      301 cgcgagcaca aagaaccgcg tgacctaagc ccagcggttt cttttgccga actgaaacga
      361 cctcggtcat ctcagcgata cgccgaagca tatccacggt tgccgtctgc ccgcgatcgg
      421 cgaggatttg ctccaactcc ggcgcctcat caaaatgatc ctcgatgcca tccttgccgc
      481 ggccggttac aaagatgatt tcttggatac cggcatcgac cgcttcctga accacatact
      541 ggatcgaagg aatatcgatg atcggcaaca gttctttggg aacggttttc gtcgccggta
      601 ggaatcgcgt gcccaagccg gctacgggga taactgcttt tctaattttc atgatttagc
      661 gaaaccacca cttacatccg tttggccttt ctttcggcga tttcctggtt ggccttgcaa
      721 tcgatacaga gagtcgtcac cgggcgggcc ttgaggcgtt caatgccgat cttttcgccg
      781 cactcttcac agatgccgaa ttcgccggct tccagtcgtt gcaaagcctc ctgaattttg
      841 aagatcagct ttcgctcgcg atcgcgtatt ctcaggatcg aatttcgatt ggattccatg
      901 gaagcccggt cgcttggatc gggaaagttc tcgtcctcat ccatgtcgtc taccgtcttg
      961 ccggcttcgg acctgagttc ctggactctc tggttcagca ggtcgcggaa aaactcaact
     1021 tcttttttgt tcaatggctt cagcctcgca aatcacccat gagtataggt aaccgccgtg
     1081 caaacagcaa acggcaaagt tgctacacag ggtgatcact cacccaagga caccgcacgc
     1141 gggtgcgcaa ggacaaacac ttcggtggat ttgggcagca tcgctcggct cagcaccaac
     1201 tgcgacccgg gaaatctttt caccggataa tgctcttgtg caaaactggc aaatagctct
     1261 ccctgcctta tgacatcgaa atgcatcgga atcacgatcg gcggcttcac ctgctcgatg
     1321 acctttagaa tgtcgtcgaa tcccaagttg gtcatcgcat cgataggcac catcatgaca
     1381 tcgacccgct ggagcacctg ctcctgctca gcagtgagga ggtgaccgat gttgcctaaa
     1441 tgagccaggc agagacttcc catctcgtaa ataaaaatcg agtttgccgc acctccccaa
     1501 ctctgccctc gctggcttgg aatattgatg atggtgatgt ctttgacgct ggtgcgaatc
     1561 gggttccagg tctgccgaaa cgtgatgccg cgcagaatca ccggatcacc cgtgacagca
     1621 ccggtgttgt tatgggtgaa atgatcgttg ctgacggtca ccgcatcagc ttggattccc
     1681 ggagtcacgt tgaagtttgg atccgcgacg actttggtct ggctgccgga atgaatcaaa
     1741 aagcttgaat ggccgtacca ctgaataaag tagtgaccca gctgagggct cttaccgggc
     1801 ctgacctgaa ccagttgccg ccgtggcttc tgctgcaatc gtacgagcgc cggattacag
     1861 attgccagcg cgacgctgcg aagcggatca aaaatcaacg cgatgagtag agcgacaaag
     1921 ccacgccgtt tcatggctcc tcccggataa aagcgccgtg cagatttttt gaggcggcac
     1981 actttaacgc tgcgcctagc gtcttacaag catattccca gagacgaggc cgggtcgagc
     2041 ttcgtgcagg ttcgattaag tggctgacgt gcgccttcgc tttacgcggt caaatcctcg
     2101 gccaacaatt tctgggtttc cggctgcagc acaacggccg cttggtagtt tcgatgatcg
     2161 ataataagct cggcttttgc gccggaaaca aaggtctcaa gcgcctgatc atcaaaacgg
     2221 aatcgaacat actgcaccgc gctaatctta tcttctttgc ttcgaccggc ttcgaaacac
     2281 gctgcaagcc gatgcccagc catgcgcaag gagattcctt cctcgatacc ctggaatttc
     2341 atcagttcct cgcgcaagtg agtttggtcc tcgatctcga gaaagagcgt tgcgctgagt
     2401 tctcgtgagt cagggatcag gtgattgtag gttgcaatct cctggcgaac tttgtccaaa
     2461 tccgtgattc tctcgactcg caacatttct tgtatctgaa agataaccgt atcacgattc
     2521 tcaaaaacta aagacacgcg gtcgcccaat gagatccgcc gcttttgttt gagttcgatg
     2581 atccgccggc gaaaatcctg ccgtatcttt tcataaccgg caagaccgac aatgtcatcg
     2641 attgagactt ttctcatccg tcttgccgta acgctaaccg gcggtggtaa gaaaatcagc
     2701 ggcctcgaaa ataataggcg gcgagagatt cagctgccga gtccgtccaa tcccttgtta
     2761 aatcgcccgg cgtgagactt ctccgctttc gccagagttt caaaccattc cgccagttcg
     2821 aagagacctt catcacgggc agtcttcgca aaacccggat acatctcggt gtattcgtag
     2881 gtttcacctt caatagccga tttcaggttt ttctcggtgt tgccgatagg cactcccgtg
     2941 cacgggtcac ctacttcctt cagaaaatcg agatggccga aggcatgtcc ggtttccgcc
     3001 tccgaggtgt cacggaacag tcctccgata tcagggtagc cttcgatatc ggctattcgc
     3061 gcaaaataga ggtagcgacg attggcttgg gcttcaccgg caaacgcttc cttcagattt
     3121 tgttgactct tggttccggc taaacttttg cccatcaaaa ttcctcctta tgtacgacga
     3181 ttagtgatag taaagccgat tttcgctgtc aagcaactgt caaacggggc tactcccatc
     3241 gtgctgaagc gcatttgccg tcggatcgcg gcatgaaagc ggcccgtctg gatgtctcaa
     3301 gataaatagt tgcgatgatt ccgccagctc atccgatgtt tctttttttg gctgcactgt
     3361 tctgttatcc tgttgtccgg ccctatggag gtgcgatgat ctcgatagac gcgtttagac
     3421 aaattgaact caaggtggct acagttaaaa gcgccgagcc gcatcccaac gcggacaaac
     3481 tcatggtgct ccagattgac ctggggacgg aacaacggca gatttgcgcc ggcattcgca
     3541 atcaatacac accggaagag ctcgtcggca ggcaaatcgt cgtggttgcg aacttagaaa
     3601 ccgcaaagct gcgtgggctg gagagtcaag ggatgctgct ggcggcctcc gacggagagc
     3661 gggtcatctt cctgaccccc gagaaatctg ttcaatcggg ctcgaaggtt tcttgacgat
     3721 gagcatgcac aatgtttagc acccaccttg gacgcccgac cgtttgctac atcgatcacg
     3781 aagcgctgcg ct
//
LOCUS       DASUTG010000397         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_210571, whole genome shotgun sequence.
ACCESSION   DASUTG010000397 DASUTG010000000
VERSION     DASUTG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210571"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..407
                     /locus_tag="VF089_09100"
     CDS             168..407
                     /locus_tag="VF089_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6174159.1"
                     /translation="MQLELYFYNECGFSQSVLNCMTNLGCEDKITKKNIRENPDYEKE
                     LIALCGAKTVPVLVIDGKPLRGSEEISQYLVGKFL"
     gene            complement(525..1322)
                     /locus_tag="VF089_09105"
     CDS             complement(525..1322)
                     /locus_tag="VF089_09105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6174160.1"
                     /translation="MLTRIKIDNRMDGFLGKPAGAGKRPVVIHLHERYGIVQHTTDLA
                     EKFVEAGYVTIAPDLFSRFTGDRAALAAGNERCDLDDEQVVGDIDAVVAYLRTVPEVD
                     TTRIALSGVCQTGRQPILMAAKRDYICAAAVLYGAVYDADWKSDPRRPEPIDTLIEQM
                     KCPMVGIFGELDNLIPLNNIIRMIEVLAKAKKSFDVRVYPDAPHGFLNDTMPGRFRPE
                     PAQAAWKQITNFLEAVFDGKFSKERAVWRFESDSSVNYDFTKMKRWE"
     gene            complement(1422..2492)
                     /locus_tag="VF089_09110"
     CDS             complement(1422..2492)
                     /locus_tag="VF089_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174161.1"
                     /translation="MSAVRYPEGNCMKIFHTLLSSWLLLAVLSSDAHPQSDPFFKGKQ
                     IKIMVGFTAGGIVDLWARLFTQYMGKYIPGNPDVIVQNVPGGGSMIAANQLYNIAKPD
                     GLTLGMVSTGLYFDQLMGRKEVQFDWGKFGWIGSPVRNFEVLTMRADTPYRTIEDIQK
                     ASQPPRCGTTGTGATGHYFPKFIDESLGANIQIVLGYPGNRDVEVAIERGEVHCYAIT
                     KEAFLRDPGRSWLKKGFVRALVQGGQKRDPLFPDTPTIYELMDKHKTPEELKRFASVL
                     LSPGAIGRPLMTPPNLPAERLKLLRDGYAKMIADPDFLADAKKREWDVEYISGEDLEA
                     MAKKAVIQSPDTIARLKKILAE"
     gene            complement(2515..2658)
                     /locus_tag="VF089_09115"
     CDS             complement(2515..2658)
                     /locus_tag="VF089_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174162.1"
                     /translation="MNPESAAMFQKHSYSVSCRITRLLRRNSLLGEEIIAIVAAVGKG
                     IHD"
     gene            complement(2655..4286)
                     /locus_tag="VF089_09120"
     CDS             complement(2655..4286)
                     /locus_tag="VF089_09120"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6174163.1"
                     /translation="MFPDNHSRIPVLIIGGGPVGLTLALELGWRGIRCLLVEPNEHFS
                     GNPKAKTVHQRTLELFRRWGRGIPDKLRAAAPLGADFPSTILYVTRLTGQLITALRNA
                     IPADSGNRFSPERTLRIPQSFLEPVLREEVRQMPAVILRLGWKLECFEQRPREVRAEI
                     VHVQSGARQEICAEYVAGCDGGRSTVRKQLGIRLDHWPGLQHALGGVFRAPTLWSTLT
                     FEKAFHYNVLNDDLPHLAVVGPLHPPDVWFFDLMGIDPDTLDPAEVIRNIVGQPIPFE
                     LQHVAPWTVHNALAERYRQGRVFLLGDAAHLQSPSGGYGMNGGVGDAVNFGWKLAAVL
                     QGWGGEDLLDTYEIERRQFHVRALEESIQNSEDNELLTPGLEDPIEGPTLRQTLAEKI
                     QQTKPKNFQSLGVSLGYRYAISPVVLPDGTPPTPYETSRYIPTAQPGHRAPHIWLSDG
                     TALFDHFGQGFTLLKLGSHEAGCSSLERAACNRGVPLTIVRRSEPELRELYQAGLVLI
                     RPDQHVGWRSDAEPENPAAVIDTVRGAFNEAEHNP"
BASE COUNT          973 a         1253 c         1266 g          997 t
ORIGIN      
        1 tttctcgagg ataagggaat caccgaccgc gccaccgtca tcatcgataa acaaggcgtg
       61 gttcgctacg caaaatccgt cactcctgcc ggcgagagga acccaaagga acttttgacg
      121 gaagcgcaaa aagttaataa cgaaagctcg aagcgttaag cagaaatatg cagttggaac
      181 tctacttcta caatgagtgc ggcttttccc agagcgtgct gaattgcatg accaacttgg
      241 gttgcgaaga caagatcacg aaaaagaata ttcgggagaa tcccgactat gagaaggagt
      301 tgattgcgct ctgtggcgca aaaacggttc ccgttctcgt gatcgatggt aaaccgctgc
      361 gcggttccga ggaaatcagc cagtatcttg taggaaaatt tctctaacca agagcaagta
      421 ctgaaaccaa aaggggagcg gcgaaaaccc gctccctttt ttatttggtt atcatgatca
      481 ttgtttgtct gagatccgca actgaggctc tttttcgatc tgattcactc ccaccgcttc
      541 atcttggtga aatcgtaatt caccgacgaa tcgctttcga aacgccatac cgcccgctct
      601 ttgctaaact ttccatcgaa gacggcttcc aggaagttag tgatctgttt ccaagccgct
      661 tgcgccggtt ccggacgaaa acggccgggc atggtatcgt tgagaaagcc atgcggcgca
      721 tcgggataca cgcgaacgtc aaaacttttc tttgccttgg ccaacacctc gatcattcga
      781 atgatgttat tcaatggaat cagattatcc agctcgccga aaattccgac cattggacat
      841 ttcatctgct cgatcagcgt atcgatgggt tcgggccggc gtggatcgga cttccagtca
      901 gcatcgtaga cagcgccgta aagcaccgcg gcggcgcaga tatagtcgcg cttggccgcc
      961 atcagaatcg gctgtcgtcc ggtctggcac acgccggaga gggcaattct cgttgtgtcg
     1021 acctcaggta ctgtacgtag ataggcgacc accgcatcga tgtctcccac aacttgttcg
     1081 tcgtccaggt cacatcgctc gttacccgct gccagcgcgg cgcgatcgcc ggtaaagcga
     1141 gagaaaagat ccggcgcgat cgtcacatat cccgcttcga cgaatttttc tgccagatcc
     1201 gtggtgtgtt gcacgatgcc gtagcgctcg tgcaggtgaa tgacgacggg gcgtttgccg
     1261 gccccggccg gcttgccgag aaaaccgtcc attcggttgt cgattttaat acgtgtaagc
     1321 atatatcttc tcctgctgtg gtaaggcacc aagatgcccc acagacatgt gtggtttttc
     1381 taaacgggcc gaaggtccaa gtcgatcctg cgcagaatgg ctcattccgc caatatcttc
     1441 ttcagtcgcg cgatcgtgtc cggcgactga attaccgctt tcttagccat tgcttccaga
     1501 tcttcgccac taatgtattc cacatcccac tcgcgttttt tagcgtcggc gagaaaatcc
     1561 gggtcggcga tcattttagc gtagccatcg cgcaggagtt tgagtctttc tgccggcaga
     1621 ttcggcggcg tcattagcgg ccggccgatg gcgccgggcg agagcagcac cgacgcaaaa
     1681 cgtttcagct cctcgggagt tttgtgtttg tccatgagct catagatggt cggtgtatcc
     1741 ggaaacagag gatcacgttt ttggccgcct tgcaccaggg cgcggacaaa tcctttcttc
     1801 agccacgaac ggccggggtc gcgcaggaac gcttccttgg tgatcgcgta acagtgaacc
     1861 tcgccgcgtt cgatggccac ttcaacgtcc cggttgccgg gatagcctaa cacgatttga
     1921 atgttcgccc ccagcgactc atcaatgaac ttgggaaaat agtggccggt ggcgcctgtt
     1981 cccgtggtgc cgcagcgcgg cggttgcgac gctttctgga tatcctcaat ggttcgatac
     2041 ggcgtatcag cgcgcatcgt caatacttcg aaattgcgca ccggcgaacc aatccagccg
     2101 aacttacccc aatcgaattg aacctccttg cgtcccatca attgatcgaa gtagagtccg
     2161 gtggaaacca ttcccagagt caacccgtcc ggttttgcga tattataaag ctgattagcc
     2221 gcaatcatcg agccgccgcc gggcacgttt tgaacgatga cgtccggatt gccgggaatg
     2281 tatttgccca tgtattgggt gaacaaccga gcccataaat cgacgatgcc accggcagta
     2341 aagccgacca taattttgat ttgcttacct ttgaagaatg ggtcggattg gggatgggcg
     2401 tcgctcgaca ggacagcgag taagagccaa ctacttaaca gcgtgtgaaa tattttcatg
     2461 cagtttccct cagggtagcg aacagctgac ataactcggc tgctggcccg ctatctaatc
     2521 gtgaatgcct ttacccacgg ctgcgacaat ggcaatgatc tcctcgccga gtagtgagtt
     2581 tctgcgtagc aagcgcgtaa tgcgacacga tacgctgtaa ctatgcttct gaaacatggc
     2641 tgcggattca gggttcatgg gttatgctcc gcctcattaa atgcgccgcg aaccgtatcg
     2701 atcacagcag cgggattctc cggttctgcg tcggaacgcc agccgacgtg ctggtccggg
     2761 cggatgagga ccaggccggc ctggtagagc tcgcgaagct caggctcgga acgtcgcacg
     2821 atggtgagcg gcactccacg gttgcaggcg gcacgctcga ggcttgagca gcccgcctca
     2881 tgggatccga gcttcagaag ggtgaaacct tggccaaaat ggtcgaataa cgcggtgccg
     2941 tcagagagcc agatgtgcgg ggcgcggtgg cccggctgag ctgttgggat gtagcgcgag
     3001 gtctcgtatg gcgtcggtgg cgtaccgtcc ggcaaaacga cgggcgagat cgcataacga
     3061 tagccgaggc ttacgcccaa actctgaaag ttcttaggtt tggtttgctg aatcttctcc
     3121 gccaaagttt ggcgtagagt tggaccttcg atagggtctt cgagacccgg tgtaaggagt
     3181 tcgttatcct ccgagttctg gatcgactcc tccagggctc ggacatggaa ttgacgtcgc
     3241 tcgatttcgt acgtgtccag caagtcctcg ccgccccagc cttggaggac ggctgccaat
     3301 ttccagccga agttgacggc atcgcccacg ccgccgttca tgccgtagcc gccgctcggc
     3361 gactgcagat gggcggcatc gcccaacagg aagacacggc cttgacggta gcgctcggca
     3421 agcgcgttgt ggacagtcca cggtgcgacg tgctgtaact caaaagggat cggctggcca
     3481 acgatgttac ggatgacctc agctggatcg agtgtgtcag ggtcgatgcc catcaagtcg
     3541 aagaaccaca cgtccggcgg gtgcagcgga ccgacgacgg caaggtgcgg caagtcgtcg
     3601 ttcagaacgt tgtagtgaaa cgccttctca aaagttagcg tagaccacaa cgtcggagcg
     3661 cggaaaacgc ctccgagcgc gtgttggagg cctggccaat gatccaggcg gatgcccaac
     3721 tgctttctca ccgtgctccg gccgccgtcg caaccggcca cgtactcggc gcaaatttcc
     3781 tggcgcgctc cgctctgcac gtgcacgatt tccgcccgga cttcgcgggg ccgctgctcg
     3841 aagcactcca gcttccaacc gagccggaga atgacagccg gcatctgccg tacttcctca
     3901 cgcaggaccg gttctaaaaa cgactgcgga atgcgcaatg ttcgttcggg actgaatcga
     3961 ttgccactat cggcgggaat ggcgttgcgc aacgccgtga tcagctggcc cgttagccgc
     4021 gtaacgtaca agatggtcga gggaaagtcc gcgccgagag gcgccgccgc acgcaatttg
     4081 tcgggaatgc cccggcccca acgacgaaac agctccagtg tccgctggtg caccgttttg
     4141 gctttgggat tgccgctgaa gtgctcatta ggttcaacga gcaggcagcg gatgccgcgc
     4201 caaccaagct cgagcgcgag tgtcaatcca actggtccgc cgccgattat caggaccggt
     4261 attctgctgt gattgtcagg gaacatttca gttctagatc cttcgatcgc ttggatcgcg
     4321 aaaacttccg ttcgaggacc ttttaatcac tcaatcttca ttatcccgta cgacgtccgg
     4381 ccggggcctg ttagttctcg ggttcgtact gcagattgtc gcggatcaag ttagagattc
     4441 cgcgggcgat cggccggcgg atttcttcgc cgacatgagc gagcccgcc
//
LOCUS       DASUTG010000398         4041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1692572, whole genome shotgun sequence.
ACCESSION   DASUTG010000398 DASUTG010000000
VERSION     DASUTG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692572"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1335
                     /locus_tag="VF089_09125"
     CDS             304..1335
                     /locus_tag="VF089_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174164.1"
                     /translation="MIVALMLFVGLFFCNISAEAQTPFYQGKTITVIIGTPPGNLYDL
                     WGRLIVEHMGKHIPGNPAFIVQNMPGAGHVIAANHLYKVAKPDGLTITGSVIPSLYLN
                     QLIGRPEIQFDWSKFSWIGSPARGASQMYMRSDTPYKTIEDVRTAKEPPKCGATGVTG
                     PDSYYPKLLQETVGAKFTIVSGYPGGTDIDLGVERGEIHCRAFTIEAFFGREPYHTWR
                     KRGFVHHLFQSGKTRDSRLPNTPTIFEIMDQYKTNESGRRLATVILGADGMGRPMFGP
                     PGMPADRLAILRDAFTKTMADEQFRAEVKRRNYEFDPIAGEELQDLSKEVTSQPPETV
                     ERLKKLLSN"
     gene            1608..2144
                     /locus_tag="VF089_09130"
     CDS             1608..2144
                     /locus_tag="VF089_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6174165.1"
                     /translation="MPNEKNSIGEVQEAIRRGAITREYKADTNRVIQALNGLRSTEIM
                     SYLQYMQHQYMAVSLLSPGLKTEFEAHATQELDHANRLAERIQQLGGVPVYDPKEIST
                     KAAGVGVVPEQGATLTEMVVEDLLLERQQIEVYSALIREIGDEDLVTRHVLLGILADT
                     EKHASELADYLKRTADTH"
     gene            2205..2429
                     /locus_tag="VF089_09135"
     CDS             2205..2429
                     /locus_tag="VF089_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HEX6174166.1"
                     /translation="MARKIEPGDRVEWNTPQGKTTGKVKKKLTSPKQIKGHVAKASKD
                     NPEYLVESEKSGKEAAHKPRELRKVRTRRK"
     gene            2479..3864
                     /locus_tag="VF089_09140"
     CDS             2479..3864
                     /locus_tag="VF089_09140"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX6174167.1"
                     /translation="MIQSQVASERIQLLNSKALARGVHVLYWMQQSQRAEYNHALEYV
                     IEQANELKLPVRVVFGLTDDYPEANLRHYTFMLEGLSETQAALTKRGIPMSVQKGDPA
                     KVALKAAREAALLVCDRGYLRHQKRWRRQVAEAAPCRVVQVESDVIVPVRAVSDKREV
                     AARTLRPKIHKQLGKFLVEMAETKVARRSAPGELAGIDLNDLEGTLKLLKIDRSVHPV
                     GRFFTGGLSQAKNRFAIFLQRKLAHYRDNRNQPQTDYVSHMSPYLHFGQISPLYLALR
                     VWRRRAADRANAETYFEELVVRRELSMNFVEFTDVYDSITCLPEWAKETLNNHRRDTR
                     EQLYSPAEMERAKTHDRYWNAAWLEAKRTGYMHSYMRMYWAKQILRWTRSPEEGFDTA
                     LALMNKYFLDGRDANSYAGVAWVFGNHDRPFYDREVFGTVRSMTASGLERKADMDAYI
                     AKVESFRNSAE"
BASE COUNT          989 a         1120 c         1094 g          838 t
ORIGIN      
        1 cgttttcttc aggcccgccc gcccgcggtc aatggcatcg acctcctgaa cccgcgtgtt
       61 gtcggctgcc ggtatttgag aaccgcgccg gctatctcga tgccatgttt tccaatccaa
      121 tcgggtcgcc actatccaac ctatgctcac gaattaggag tctgcgggcc acagatagac
      181 cccgtcacga ttatgcattg acccatttct cccttgacgc ttgagtgcag tttcgattag
      241 cctctggccc actggacctt ggtcgtaaaa ccaatcgccg cttccaacgt gatgaggtgc
      301 aacatgatcg tagctttgat gctgtttgtc ggtctgttct tttgcaacat tagcgcagag
      361 gcacagacgc ccttctatca ggggaaaacc attaccgtga ttatcggcac gccgccgggg
      421 aatctgtatg atctctgggg gcgtttgatc gtcgaacaca tgggcaaaca tattccgggc
      481 aacccggctt tcatcgtgca gaacatgccc ggcgctggcc atgtgattgc agccaaccat
      541 ctctataagg tcgcgaaacc ggacggcttg acgattaccg gctcggtgat tccctcgctc
      601 tatctgaacc agctgatcgg cagacccgag attcaattcg attggtcgaa gtttagctgg
      661 atcggctcgc cggcccgtgg cgccagccag atgtacatgc gcagcgatac gccgtacaaa
      721 actatcgaag atgtccgcac cgccaaagag ccgcccaaat gcggcgcgac cggcgtcacc
      781 gggcccgact cctactaccc gaagctgttg caagaaaccg tgggtgcaaa attcaccatt
      841 gtgtcaggtt accccggtgg caccgatatc gacctcggcg tcgagcgcgg tgaaattcac
      901 tgccgcgcct ttaccatcga ggcattcttt ggccgcgagc cgtaccatac gtggcgcaaa
      961 agaggcttcg ttcaccatct gtttcagagc ggtaaaacac gcgactctcg gttgccgaat
     1021 acgccgacaa ttttcgagat catggatcaa tacaaaacca acgaatcggg acggcggctt
     1081 gccaccgtaa tactcggcgc cgatggcatg ggacggccga tgtttggacc gcccggcatg
     1141 ccggctgatc gtttagcgat tctgcgcgac gccttcacaa agaccatggc cgacgagcag
     1201 ttccgtgccg aagtgaagag gcgcaactat gagttcgatc cgatcgcggg cgaggagttg
     1261 caagacctgt cgaaggaagt aaccagccaa ccgcccgaaa ctgtagagcg tttaaaaaaa
     1321 cttttgtcga actgacatat cggcgagaaa attcttgctg agagttcccc ggagcttatt
     1381 tggcacctgc gagttcggaa aacctgtacg aagcgaactg acggcctaca tcatagatca
     1441 cgtcccgagt tcgggactac tctgtatcga tttgcattat ctttgagacc cgcgctggag
     1501 atttctaaat ttttcgcgca ctagaaagca gctctcttgg tattgatctt gcgccttcta
     1561 cgacagcggc agcgtgaaag tgctgcttcg gaacgaaagg agtaattatg ccgaacgaga
     1621 aaaatagcat tggggaagtt caagaagcaa tcagacgagg agctataacg cgcgagtata
     1681 aagccgacac aaaccgggtc atccaagctt tgaacgggct ccgatcaacg gagatcatga
     1741 gttatctaca gtacatgcag catcaatata tggccgtctc cttgctgtcg cccggtctca
     1801 agaccgagtt tgaagcccat gcgacgcaag agctcgatca tgccaatcgg ctggctgaac
     1861 gaattcagca actgggcggc gtgcctgttt acgacccgaa ggagatttcg acgaaagctg
     1921 caggtgttgg tgtcgtaccg gagcagggcg cgaccctaac cgaaatggtt gtggaggatc
     1981 tcttgcttga acgacagcag attgaagttt acagcgcctt gattcgcgag attggtgatg
     2041 aagatctggt aacccggcat gtgctgctgg gcatactcgc cgacacggaa aagcacgcca
     2101 gcgaattggc cgattatctg aaaagaaccg cggacacaca ttgatcaacg gacgggatcc
     2161 aatagctttg ccgaaaccta ccgctcgcat acagctgcat ttccatggct aggaagatag
     2221 agcccggcga tcgcgtcgag tggaacactc cccagggaaa aaccaccggc aaagttaaga
     2281 agaagcttac ctcccctaaa cagatcaagg gccacgtcgc caaagcatca aaagacaatc
     2341 ccgaatatct ggtcgaaagc gagaaatccg gcaaggaagc cgcccacaag ccccgggagt
     2401 tgcgaaaggt gcgcacccgg cgtaagtgaa ccgttacccg gcggccgaca agtgcgccgg
     2461 ccggcgatct cgtcaacaat gatacaatcc caggttgcct ccgagcgtat tcaattgctc
     2521 aactccaagg ccctggctcg tggcgtgcac gtactttact ggatgcaaca gtcacaacgc
     2581 gccgagtaca accatgcatt ggaatatgtc attgagcaag ccaacgagct aaaactgccc
     2641 gtccgtgtgg tttttggtct gactgacgac tatccggaag ccaacctgcg acattatacg
     2701 ttcatgctgg aaggattgag tgagactcaa gctgcgctca cgaaacgcgg catcccgatg
     2761 agcgtacaga aaggagatcc ggccaaggtc gcactgaaag ccgcgcgcga ggccgcgctc
     2821 ctggtctgtg atcgcggcta tctgcgacac cagaagagat ggcgccggca ggtcgccgaa
     2881 gctgcgccgt gccgagtcgt gcaggtcgag agcgatgtga tcgtgcccgt cagggcagtt
     2941 tcggacaagc gggaggtggc cgcgcgcaca ctccgtccga agatccacaa acagctgggt
     3001 aagtttctcg tcgagatggc cgaaaccaaa gttgcgcggc gcagcgcacc gggcgaacta
     3061 gctggaatcg atcttaatga tctggaaggg actctaaaac tgctcaaaat agatcgcagc
     3121 gtacatccgg taggccgctt ctttaccggc gggctatcgc aggctaaaaa ccggttcgcc
     3181 atatttttgc aaagaaagct cgcgcactac agagacaacc gcaatcagcc gcaaaccgat
     3241 tacgtgtcgc acatgagccc atacttacac ttcggccaga tctcgcctct atatctggcg
     3301 ttgcgcgttt ggcggcggcg cgcagccgac cgcgccaatg ccgagacgta ctttgaggag
     3361 ctggttgtga ggcgcgagtt atcgatgaac ttcgtcgagt tcacggacgt ttacgattcg
     3421 atcacgtgct taccggagtg ggcaaaagaa actctcaaca accaccgccg cgacacccgg
     3481 gagcagcttt attcgccagc ggaaatggag cgggctaaga cgcatgatcg ctattggaat
     3541 gcggcatggc tcgaagccaa gcgaacggga tacatgcaca gctacatgcg tatgtattgg
     3601 gccaaacaaa ttttaagatg gacgcgctcg ccggaagaag gtttcgatac cgccctagct
     3661 ctgatgaaca agtattttct tgacggccgg gacgccaatt cgtacgccgg tgtcgcctgg
     3721 gttttcggca atcatgaccg gcccttttac gatcgggaag tttttggaac ggttcgctcc
     3781 atgaccgcat caggcctgga acgaaaagca gatatggacg cttatatcgc caaagtagaa
     3841 agctttagaa acagcgcgga ataaatgccg ccgcgcgtga acctagaagc acctcgttca
     3901 agcttctcga catccgcact taccaggcaa gtagtctccc tttagggctt attgtcatag
     3961 ccgcaatcgc ggttgatgaa gcactagctc tacaaactcg taatcctctt cgcgcactca
     4021 tttctccagc cgatccagga t
//
LOCUS       DASUTG010000399         5650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1842090, whole genome shotgun sequence.
ACCESSION   DASUTG010000399 DASUTG010000000
VERSION     DASUTG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842090"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF089_09145"
     CDS             <1..402
                     /locus_tag="VF089_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174168.1"
                     /translation="VADLGVEVQGNGFATSRSYIASNRDTVKNALKGFVEAIFFVYAN
                     KKEAQQVFAKYMRTNDPEILEDSYNGYIKWIPKKPYPTLKGIQYMLDIVAPQLPQAKN
                     AKPEQFVDLSFLQELEKEGFFNEMAKRYPSK"
     gene            448..837
                     /locus_tag="VF089_09150"
     CDS             448..837
                     /locus_tag="VF089_09150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6174169.1"
                     /translation="MATKTIQPSSLNDPRPRYSQGIQAEGGRLLFIAGQTASDKNGNV
                     VGKGDIKAQTRQVFENIKAVLEAAGGSLDNLVMTTTYITDRSYREGYNEVRRGLYKKD
                     PPTSTLLIISGLANEDYMIEIAGIAVL"
     gene            1000..1977
                     /locus_tag="VF089_09155"
     CDS             1000..1977
                     /locus_tag="VF089_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX6174170.1"
                     /translation="MRVTVLDDYQHALATTPAIKRLQQHAEVQILTEKLPSQDALVRA
                     LAGSRAVIPIRERTRFDASVLKKLPDVELISQTGNHVYHVDMEAATEAGIIVAMAPGG
                     NSTTEMAFALMLAAMRRIPQSDQAVRRGEWPLVLGYVLKGKTLGILGLGKIGTEVAAI
                     ARAFDMNVIAWGPTLTAERAGKSQATYMGLEEVLRIADVISIHLKLSEQSRNLLNEER
                     LRLMKKSAYLVNTARGAIVDETALVKVLSEGAIAGAALDVFVEEPLPPDHPLLQLSNV
                     VLTPHLGWPTDSGFAGFAENAVTNILDYMDGKLTRVINPEALEHRASRK"
     gene            complement(1987..3243)
                     /locus_tag="VF089_09160"
     CDS             complement(1987..3243)
                     /locus_tag="VF089_09160"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX6174171.1"
                     /translation="MLTGAGLVMPGLLSQASCAPTLIGTDRDTGLSLCYVAGDVTSDS
                     AIIWLRAEPGSEVTLHYSNDRTFTRFSSAGPFQVDEEADHTAKIELQKLDPGTTYFYY
                     AAVAGKKPGYLASFLTAPAASDAVKVSFCFSGDTRESYQPFTIMNAVRAQRPDFFLHL
                     GDTIYADIGGPARRLSDFWAKYRANRNDPATQLCFFETSTYVVWDDHEVVNDYFGSNP
                     LALPGRKAFFDYWPIRRHPGEADRIYRSFRWGRAMELFLLDTRQYRDPGAGTMLGKAQ
                     KDWLFKSLTASSAMFKFVATSVPMAGGGSDRWDGYPAERAALLRFIREKRITGVIFLS
                     ADLHYAAVTSVPKSHGLKDITAGPLAAPLNRVTNGYAPRFDFFLAENFNFAKITVDPA
                     LKPARALVEFIDQDNAVFHTTTIKAV"
     gene            complement(3275..3781)
                     /locus_tag="VF089_09165"
     CDS             complement(3275..3781)
                     /locus_tag="VF089_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6174172.1"
                     /translation="MDTSTIVMLAAAIVALIVIYWKSPQAAGDGLTATGALIIEIIPR
                     MIAAFTLAGLIQAVVPQEVIVRWMGHGSGAKGILIGMTLGSITPGGPMTHFPVVASLY
                     KVGIGVGPLVAYLTAWSLFGLQRVIMWEIPFLGPKIVAVRIAVSFFFPLLAGWLCELI
                     WNKLQLQT"
     gene            4129..5412
                     /locus_tag="VF089_09170"
     CDS             4129..5412
                     /locus_tag="VF089_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6174173.1"
                     /translation="MTIDQEAANKVLAEIDRDELAQLGCDLTSIPSPTGQEKAIGEFI
                     LNWFQANGLKAVRQDVEFDRPNAVGIVRGDGSGLSLGFNGHTDTSFTGTAEDARMVAN
                     MEPESELKGKIVGNKVQGLGISNMKGGVAAFMMAGKALKKSQVKLKGDVILAAVVGEI
                     SRTPIGPWQTKEYRGEGAGTRHLLTHGMHSDYAVVADGSDLNIVWTQTGVVQVKIQTF
                     GKAEAAWGSKRSTHPMEKMNAIVKMTKIIEAVERWGAEFEDKYVYNSPTGPLYPKVNI
                     GGIEGGAPYRPNYFPGVCTIYVDVRMPPQVRPVTIKYELETALNRLGLEYDMEIYKSL
                     LGHEGKNVEPLVKSAEEVYQHLFKEKIKPEAADRASIWTDTNVYNELGIPAIKIGPRG
                     RRIGPRNEEIEIDVMVKAAQIYALMALDICSRERP"
     gene            complement(5444..>5650)
                     /locus_tag="VF089_09175"
     CDS             complement(5444..>5650)
                     /locus_tag="VF089_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174174.1"
                     /translation="YTRDNDPVTLEETYEFFTKQAGFNKDLTFSDRGFEQILKFLGGT
                     VLPAAKDAPVNHFYDTRILEKLKK"
BASE COUNT         1411 a         1564 c         1544 g         1131 t
ORIGIN      
        1 gtcgccgatt tgggggtcga ggttcaggga aacggctttg ccacatcgcg ctcctacatc
       61 gccagcaatc gtgacacggt aaaaaacgcg cttaaggggt tcgtcgaagc gatttttttt
      121 gtttatgcca acaaaaaaga agcgcagcag gtgttcgcca aatacatgcg cacgaacgat
      181 cctgagattc tcgaagattc ttacaatggc tacatcaaat ggattcctaa aaagccctac
      241 cccacgctta aaggaattca gtacatgctc gacattgtcg cgccgcagct gccgcaggcg
      301 aaaaatgcca agcccgaaca gttcgtcgat ctgagttttt tacaggaact ggaaaaagaa
      361 ggcttcttca acgagatggc gaagcgctat ccgagcaagt agcaaagctt caaatctaaa
      421 caaatcgcgc acaaggagga gagatttatg gcaactaaaa ccattcaacc gtcgtctttg
      481 aatgatccgc ggccgcgcta cagccaggga atacaagcag agggaggaag gctgcttttt
      541 atcgccggcc aaaccgcttc ggataaaaac ggcaatgtcg tcggcaaggg agatatcaaa
      601 gctcaaactc gccaggtgtt cgagaacatc aaagcagttt tagaagccgc cggcggctcg
      661 cttgacaatc tggtgatgac caccacctac attacggatc gaagctaccg cgagggatat
      721 aacgaagtgc gccgcgggct ctacaaaaag gatccgccca ccagcacgct tctgatcata
      781 agcgggctcg ccaacgaaga ctacatgata gagatcgccg gaatcgccgt cctttagcag
      841 tttacagaaa aattctgaat gcaggctgct caaaaagatc tcagaggtga ggcgcgcgaa
      901 gttcgaagag ctgaggcgta cgcttcagta cgttgaagcg aatcgggcga gcgcaacgaa
      961 gcatatgagt ctttttcagc ggctcgctga tttaagttta tgagagttac agttctcgac
     1021 gattaccagc atgcgcttgc aaccacgcct gcgatcaaac gcctgcagca gcacgccgag
     1081 gtccagatat taaccgagaa attaccaagc caggacgccc tcgttcgcgc cctagccggc
     1141 tcccgcgcgg tgattccgat ccgcgagcgc actcgatttg atgcgtctgt attaaaaaaa
     1201 ttgccggatg tcgaattgat ctcgcagacc ggcaatcatg tttatcatgt cgacatggag
     1261 gcggccaccg aagccggcat catcgtcgcc atggcgcccg ggggcaacag cactaccgag
     1321 atggctttcg ctctgatgct ggccgcgatg cgccggattc cgcagagcga tcaagcagtg
     1381 agacgcggcg agtggccgct ggtgctgggc tatgttctca aaggcaagac cctggggatt
     1441 ctcggtttgg gaaaaatcgg caccgaggtt gccgctatcg cgcgcgcttt cgacatgaac
     1501 gtcatcgcct gggggccgac cttgacagcc gaacgcgccg gcaaatcaca ggcgacctac
     1561 atgggactgg aagaggtcct gagaatcgcc gacgtcatct cgatccacct gaaactctcc
     1621 gaacaaagca ggaatcttct gaatgaagag cgccttcgct tgatgaaaaa atccgcttat
     1681 ctggtcaaca ccgcgcgtgg cgccatcgtc gatgagacag cgctggtaaa ggtattaagc
     1741 gaaggcgcga ttgccggcgc cgctctcgac gtattcgtcg aagaaccgct gccgccggat
     1801 cacccgctgc tccaattgag caacgtcgtg ctcactccgc atctcggctg gccaacggat
     1861 tccggttttg caggcttcgc ggaaaatgcc gtcaccaaca ttctcgatta catggacgga
     1921 aaactgacac gagtcataaa ccccgaagcg ctcgagcatc gcgcctcacg caagtagcgc
     1981 cgccgatcat accgccttga tcgtggtggt atgaaaaacc gcattgtctt gatcgataaa
     2041 ttcaactagc gcccgggccg gcttaagcgc cggatccacg gttattttgg cgaagttgaa
     2101 attttctgca agaaaaaaat cgaaacgcgg cgcatacccg ttcgtcaccc gattcaaagg
     2161 cgccgccaga gggccggcgg taatatcctt caggccgtgg cttttgggca cgcttgtcac
     2221 cgctgcatag tgaagatcgg cgctcaagaa aatcacaccg gttatgcgct tctctcgtat
     2281 aaagcgcagc aacgccgctc tttccgccgg gtagccgtcc caacggtcac taccgcctcc
     2341 ggccatcggc acggaggtcg ctacgaattt aaacattgcg gaagaagccg tcagcgactt
     2401 gaaaagccag tccttctgcg ccttgccgag catagtcccc gctcccgggt cgcggtattg
     2461 ccgcgtgtcc agcagaaata attccatggc ccggccccag cgaaacgagc ggtagatccg
     2521 atcggcctca ccggggtgac gtcggatcgg ccaataatcg aaaaacgcct ttctccctgg
     2581 tagcgccagc ggattgctgc caaaataatc attcaccact tcgtgatcgt cccagacgac
     2641 atacgtgctg gtttcgaaga aacagagctg cgtagcagga tcgttgcggt tggcacggta
     2701 cttcgcccag aaatcactca accggcgcgc cggaccgccg atatcggcat agatggtgtc
     2761 gcctagatga agaaaaaaat ccggtcgctg cgcgcgcacc gcgttcatga tggtaaacgg
     2821 ctgatagctt tcccgagtat cgccactgaa acaaaatgac actttgacgg catccgatgc
     2881 agccggcgcg gtcaggaagc tggcgagata accgggtttt tttccggcta ctgcagcgta
     2941 gtaaaaatag gttgtgccgg gatcgagctt ctgcaattcg attttcgcag tatggtccgc
     3001 ttcctcatcg acctggaatg ggccggcgga tgagaagcga gtaaaggtgc gatcgttgct
     3061 gtagtgcagc gtcacctcgc tgcccggctc tgcgcgcagc cagatgatag cgctatcgga
     3121 tgtcacgtcg ccggccacat agcacagtga caaaccggta tcgcgatcgg tgccaatcag
     3181 agtgggagca caggatgcct ggctcaagag cccgggcatg accaaacctg cgccggtcag
     3241 cattcgaaga aattctctgc gcctcacgtt tgaatcaagt ttgaagttgg agtttgttcc
     3301 agatcaactc gcacagccaa ccggcgagga gtggaaagaa gaagctaacg gcgatgcgca
     3361 ccgcgacgat cttaggtcca agaaaaggaa tctcccacat gatcactcgc tgcagtccga
     3421 agagagacca ggcggtcaga taagccacca gaggtccgac accgatgcct accttgtaga
     3481 gcgacgcaac caccggaaag tgcgtcatcg gcccgccggg cgtgatgctt cccagagtca
     3541 tgccgatgag aattcctttg gcgcccgaac catggcccat ccagcgaacg atgacttcct
     3601 gcggaaccac cgcctggatc aagccggcca gtgtgaacgc ggcgatcatg cgcggaatta
     3661 tctcgatgat caatgcccca gtcgcggtta agccgtcgcc tgccgcttgc ggcgacttcc
     3721 agtagataac gatcagtgcc acgatcgcag ccgccagcat gacaatggtg gacgtgtcca
     3781 tagatctcct taaactcagg cggaatttct ctgggataag cattgatgaa gtttcgatcg
     3841 gacgtaaagc gtagcctgca actgtaaaca gcaagggacg atttttcaag cagacggttt
     3901 tccaacgact atttgacttg ccggcggcac tatagtttct atagttttct aataggctgt
     3961 tgaaaaagct tcatgctccg ttgcgttggt tcgcttggct tggaagtact gtcgtcgtac
     4021 gcctcagctc atcgaacttc gcgcgccttg ccttcgagta ttttgagcgg cctatgaagc
     4081 agagttgtga gcaaactggc acttaagctg aagcggaagg agaacgaagt gacaattgat
     4141 caagaagcgg cgaacaaagt gttggcggaa atcgatcgcg atgagctggc acagctcggc
     4201 tgcgatctca ctagcattcc cagccccacc ggacaggaaa aagccatcgg cgaattcatt
     4261 ttgaattggt ttcaggccaa cggcctcaaa gccgtgcgcc aagacgtcga gttcgaccgc
     4321 cccaatgccg tgggcatcgt cagaggcgat ggcagcggat taagcctggg ctttaacggc
     4381 cacaccgaca ccagctttac gggcacggct gaagacgcgc gcatggtcgc caacatggag
     4441 ccggagtcag agctaaaagg caaaatcgtc ggcaacaaag tccagggctt gggcatctcc
     4501 aacatgaaag gtggtgtcgc agccttcatg atggccggca aagcgctcaa gaaaagccag
     4561 gtcaagctca aaggcgacgt catccttgcg gcagtggtag gcgagatctc gcgaactccg
     4621 atcgggccct ggcaaactaa ggagtaccgc ggcgaaggcg ccggcacccg gcatctgctg
     4681 acccacggca tgcactcgga ctatgccgtt gtcgccgatg ggtcggattt aaacatcgtc
     4741 tggacgcaaa ccggcgtcgt gcaagtgaag attcaaacct ttggcaaagc cgaagccgca
     4801 tgggggtcca agcgttcgac ccaccccatg gaaaaaatga acgccatcgt caagatgacc
     4861 aagatcatcg aagcggtaga acgctggggc gccgagttcg aagataaata cgtctacaac
     4921 tcccccacag gcccgctgta tcccaaggtc aacatcggcg gcatcgaggg tggcgcgccc
     4981 taccggccca attactttcc cggcgtctgc acgatctacg tcgatgtgcg catgccgccg
     5041 caagtcagac cggtgaccat caagtatgaa ttagagacgg cgttaaaccg tctgggcttg
     5101 gagtacgaca tggagatcta taagtcgctg ctcggtcatg aggggaaaaa tgttgaaccc
     5161 ctggtgaaat cagccgaaga agtttatcaa catctgttca aggaaaagat caaacccgag
     5221 gccgccgacc gcgctagcat ctggaccgat accaacgtct ataacgagct cggcattccc
     5281 gccatcaaga tcggtccgcg cggccgccgc atcggcccaa ggaatgaaga aatcgaaatc
     5341 gatgtgatgg tgaaggcagc gcaaatctac gcactgatgg cactggacat ctgcagccgc
     5401 gagcggccgt aggacgcttt ataccgctga cttttgccga agatcatttt ttcagcttct
     5461 caaggattct tgtatcgtaa aaatgattta cgggcgcatc ctttgcggcc ggcagcaccg
     5521 taccgcccaa gaatttaaga atctgctcga aaccgcggtc actaaacgtc agatccttat
     5581 tgaagccggc ctgcttggta aaaaactcgt aagtctcttc caacgtcacg ggatcgttgt
     5641 ctctcgtgta
//
LOCUS       DASUTG010000400         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1692690, whole genome shotgun sequence.
ACCESSION   DASUTG010000400 DASUTG010000000
VERSION     DASUTG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692690"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF089_09180"
     CDS             complement(<1..658)
                     /locus_tag="VF089_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174175.1"
                     /translation="MKSLSAALKQYGVVVLAFIHIGFGRGLHGTFGVFFVAFLDAFGW
                     TRAVTAGALSLSIIVEGIFHPVVGTLTDRLGGRKTLVLGGVVLAVGLACSATISSVWQ
                     FYFWVGIVSAAGISLIGMVPHVAIISRHYASRKATALGIAWAGGGVGIFLLVPATAAM
                     ISRWGWPSAYVGLAVVVVILVIPAVLFLLPANHVQSNTQTSGAHEIKSDKHEAQEGDW
                     T"
     gene            complement(825..2255)
                     /locus_tag="VF089_09185"
     CDS             complement(825..2255)
                     /locus_tag="VF089_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174176.1"
                     /translation="MDSRINWLLLINAALGNFMAGTASRIFTVSLPTVANSLETSIIA
                     ISWAVIAYQISQISLSLVFGRVGDVYGRHTLFGVGLVISSLGALLCGLSQNVTQLIVF
                     RLFQGVGASMTQSQGRALAMESVPKESSGRAQGYMTTAFHSGVLIGPSVGGLIIDYIH
                     WRAVFFFLIPIGLSGVLLTLFNRVKGNISARVTTPSSQRAIDYLGAILLIAATVALIG
                     SIDHRIMEAAGAAMRFVFVGGFVLLFGGFLYREITCASPILDLSLFRIRMFSLSSLTL
                     LLVGVAQVMIGFMLPFYLQDILHLTPSFIGLLFISAPIFTVTLSPFVGWAVDKVGPRL
                     PATIGISFLVCAAFLGSFLRTDSHWAWAVAVLALWGLATALFFTSNHTAMITSVPAEH
                     RGVATGAIYVMFGLGSTFGVSLGTLLLTAAFRYYSGDPTATPAPANATAFVQAMNFSF
                     FTGGIMALIAMLCSAMRGQRPAAQQG"
     gene            complement(2398..2799)
                     /locus_tag="VF089_09190"
     CDS             complement(2398..2799)
                     /locus_tag="VF089_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174177.1"
                     /translation="MSFDAQAIFADLAEKERIKGHHSAEGRAIRTLSRALNGHSSGNL
                     SGRDVVVLCDQAIEDWLKAKCKVSPWSVRGLPELLARAVQARWISRLEAVRLQKLRNA
                     RQSIDHQGADVPALEVESTLQFCLELIERHW"
     gene            3184..>3540
                     /locus_tag="VF089_09195"
     CDS             3184..>3540
                     /locus_tag="VF089_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174178.1"
                     /translation="MEPLPRGANFQRAKMLWEIGLHIAGDPHTPYYGPRDMCITVGSG
                     SNENFRPWLRMATGSPILAHAVCRNELEMAMINPSGLLTQAYRGTGLFPTPLPIRIIA
                     VYPSLDHFVYLVHPRTG"
BASE COUNT          903 a         1038 c          916 g          683 t
ORIGIN      
        1 ctgtccagtc tccttcttgt gcctcatgct tatcactttt gatttcgtga gccccgctcg
       61 tttgggtatt ggattgcacg tggtttgccg ggagcaaaaa caacaccgcc ggaatgacca
      121 gaatcacgac cacaactgcg agcccaacat aggccgaggg ccaaccccat ctgctgatca
      181 tggccgcggt cgcggggacc aacaagaaaa taccgacccc gccgcccgcc catgcgattc
      241 ccaacgcggt ggcctttcga ctggcgtagt gccgcgagat gatcgcgaca tgagggacca
      301 tgccgatgag actaatgcct gcggcagaga caatgcccac ccagaagtag aattgccaca
      361 ccgaggagat cgtggcactg caagccaggc cgacggcaag gaccacacca ccgagcacaa
      421 gcgttttgcg gccgccgagt ctgtcggtca acgtgcccac caccggatga aaaatgcctt
      481 cgacgataat cgaaagcgac aatgcacccg ccgttaccgc ccgggtccag ccaaatgcgt
      541 cgagaaaggc gacaaaaaaa acgccaaacg tgccgtgtaa accccggccg aagccgatgt
      601 gaatgaaagc cagcacaacc acgccgtact gtttcaacgc tgcggataac gatttcatcg
      661 cgtgaggatt tttcctttaa atgtcagaca tactctgcgc tccagtcaaa gtccatttga
      721 tgcacccaaa gagtaggggg gcaggagcca gatagatggg gtagtgaact atccttgcgt
      781 ggtttcgaat ccttttggga tacttcatcc ttcccgattg atcctcatcc ttgctgagcc
      841 gccgggcgct gcccacgcat cgctgagcaa agcatcgcga ttagggccat aatgccgccg
      901 gtgaaaaaac tgaaattcat agcctgcaca aacgccgtgg cgtttgccgg cgccggtgtc
      961 gccgtcggat cgccgctgta ataacgaaac gccgcggtaa gcagcaaggt gcccaacgag
     1021 acaccgaatg tcgatcccag accgaacatg acatagatcg cgccggtggc aacgcctcga
     1081 tgttctgccg gcaccgacgt gatcatcgcg gtgtgattcg atgtgaagaa cagcgccgtg
     1141 gccaatcccc acagggcgag cacggcaacc gcccaggccc agtgcgaatc agttcgcaag
     1201 aaggagccca ggaacgcggc acagactaga aaagagatgc caatggtcgc cggcaagcgc
     1261 ggccccacct tgtccaccgc ccaaccgaca aatggcgaca gcgtcacggt aaaaatcggc
     1321 gcgctgatga acaataggcc gataaaggat ggcgttaggt gcagaatgtc ttgcaggtag
     1381 aaaggcaaca tgaacccgat catcacttgc gcaacgccga ccagaagcaa agttaaactg
     1441 ctcagagaaa acatccggat gcgaaacagt gacagatcga gaatcggact agcacaggtt
     1501 atctcccgat aaagaaagcc gccaaaaagc aagacaaagc cgccgacaaa tacaaatcgc
     1561 atcgccgctc cggcagcttc catgatccga tgatcgatgc tccctatcag cgccacagtg
     1621 gccgcgatga gaaggatcgc gccgagataa tcaatcgccc gctgggatga aggagttgtc
     1681 acacgcgccg agatgtttcc tttcacgcga ttgaacagtg ttaacagaac accactaagc
     1741 ccgattggaa tcagaaagaa gaacaccgcg cgccaatgaa tatagtcgat gatcaggccg
     1801 ccgacactgg gaccgatgag cacgcctgag tgaaaggccg tcgtcatata cccctgcgct
     1861 ctgccggagg actccttggg cacggattcc atggctaatg ctcgcccctg ggactgggtc
     1921 atggaggcgc cgacaccttg aaagagccga aatacaatca actgggttac gttctgggac
     1981 aagccgcaca gcaacgcacc caacgacgaa atcaccaggc caacgccaaa aagagtgtgc
     2041 cggccgtaga catcgccaac ccggccaaag accagcgaca aactaatctg cgatatttgg
     2101 taagcaatga cggcccaaga gatcgcgatg atgctcgttt caagactatt ggccacggtc
     2161 ggtaacgaaa cggtgaaaat ccggcttgcg gtgccggcca taaagttgcc gagcgccgca
     2221 ttgatcagca gtaaccagtt gatacgtgaa tccatgttct gtcagtgaag cgcgaagcaa
     2281 aaactaattt gaaggcggca acggcaggcg gttggaataa aggagtgctc gagtatcggg
     2341 ctttaaatcc atcgatccat cactccaata ctccatttct ctgatacaac cctatcttca
     2401 ccaatgtctc tcaatcagct ctaggcaaaa ttgcaatgtc gattccactt ccagcgccgg
     2461 cacatccgcg ccctgatgat caattgactg ccgcgcattg cgtagctttt gcagacgcac
     2521 ggcttctaag cggctaatcc agcgcgcctg caccgccctg gcgagtaatt cgggcaggcc
     2581 gcgcaccgac catggcgaga ctttacactt ggccttgagc caatcttcaa tcgcctgatc
     2641 acacaacacg acgacgtcgc ggccggagag attcccggat gaatggccat tcaaggctcg
     2701 actcaacgtc cgaatcgcgc gtccttcggc cgaatggtgc cccttgattc tttccttctc
     2761 ggccagatcg gcgaagatcg cttgtgcatc gaaactcatg atcactttac gaagctactg
     2821 ctgaagcgaa cgattccaaa ccttgactgg aggtcgagtt ttaaccccct ccgtcattcc
     2881 cgcatgtcgt tagcgggaat ccatcttgct gtatggcctc tagcacccgc ttggatgccc
     2941 gccttcgcgg gcatgacgga ctttcactac gcacggcgag gtatttcaaa catccggcat
     3001 taactaccga tgaagaatac tttagcacga actttacaaa gcataccaag gttagagcga
     3061 gccatgatct aaacaacaat tcgcagtgga gtaattttca gtctcaagag cttcgcgctg
     3121 ttgacaccga cgagccgaaa tcaataaggt agcgcgggta caattacttt tctggaggta
     3181 gtgatggaac cccttccccg cggcgcaaat tttcagcgag ccaagatgct ttgggagatc
     3241 ggcttgcata tcgccggtga tccgcacaca ccctattacg gcccgcgcga catgtgcatt
     3301 acagtcggca gcgggtccaa cgaaaacttt cgcccatggc tgcgcatggc gaccggctcg
     3361 ccgattctgg cgcacgcggt ctgccgcaac gagttggaaa tggcgatgat caatccctcc
     3421 ggtctcctta cccaggcgta tcgtggaaca ggtttatttc ccacgccgct gcccatcagg
     3481 atcatcgcgg tttatccttc gctcgatcat ttcgtctatc tcgttcatcc aagaaccggc
//
LOCUS       DASUTG010000401         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2138649, whole genome shotgun sequence.
ACCESSION   DASUTG010000401 DASUTG010000000
VERSION     DASUTG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138649"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /locus_tag="VF089_09200"
     CDS             <1..1797
                     /locus_tag="VF089_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6174179.1"
                     /translation="QALRINRARRRGAKLIVIDPRKTTLAAKADCWLRVRPGTDGELA
                     MAMIHVLIEEKLFDEEFSRRWTNAAWLVRCDNHQLLTERDRQINGNPSTYLVWDGYDD
                     APVAVGRNTAAALFGSFSVVLGDGTEVNCQTALQMLKDRAAGYAPDLSEGITTVPADD
                     VRRAVRLFATEKPSSYCTWVGLEQDCDAMQTNRAVCCFYALTGQFDERGSNVLFAATP
                     TTPITGRELLAKDKADRRLGIAQHPLGPPTDPGLVQAAAVYQAILTERPYPIKALVLF
                     GSDPLLGHGDPLHGKAALEAVDFYIHVDTTLNPSATFADLVLPATTCWEHEALMPSFE
                     MAEDTLNWAQLRPAVAKPVGESRSDMAIIFDLAQRLGLAEQFFNGDIEEAFKHQLAPA
                     GIFIEELRENPIGLRANVSTRHRKYAEIDPRTGEAKGFETPTRKLEIYSTVFAQAGYT
                     PLPEFHTRADDTDPRYPLTLTFYRLVQFCDEQHRNIPRLRRSFPEPFAELHPQTAKGE
                     QIKDGEWISLETANGKVKLKAKFNGSLHPAVVAMVYGWWQACQLSNLSGYDPLSQNGA
                     NANLLIANSDVDPISASVAHRSQRCRIGKLSG"
     gene            complement(1876..2844)
                     /locus_tag="VF089_09205"
     CDS             complement(1876..2844)
                     /locus_tag="VF089_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-dihydroxyphthalate decarboxylase"
                     /protein_id="HEX6174180.1"
                     /translation="MPKPKLTIALSHYDRHVPFFDGTARPDGIDLNILIVGQSDRQRD
                     GEDRHERMLQRSEFDVAELSLSSYLMAKSRAMPFTAIPVFPRRLFSLSQMWVNVDAAV
                     HSPRDLIGKRVGVHSFQNTLATLSKGDLQSEFNVPWREIHWVLSKKDNIEFEPEKSVR
                     IDQLPDSADIGALLESGEISAMMTPHPPKPVLRGSKKIRRLFADPKAEDLRYFQKNGF
                     FPIMHVVAFKDEVLKKYPEAARSLFDTFQEAKKICREFYADPNWSWLAWGRQAFEEEQ
                     KLLGDDPWPYGLEKNRPNLERFVGYSLDQGLMARTMALEELFVPLN"
     gene            complement(2944..3285)
                     /locus_tag="VF089_09210"
     CDS             complement(2944..3285)
                     /locus_tag="VF089_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6174181.1"
                     /translation="MSSEIYVGGLPYSTTDDQLQEIFSAHGAVESARVITDKFSGRSR
                     GFGFVEMSSSDEAQKAIQALNGSDLGGRTLTVNEARPQERRSGGGFGGGGGGGGGGGE
                     RRGGGGGRNRW"
     gene            3604..>4162
                     /locus_tag="VF089_09215"
     CDS             3604..>4162
                     /locus_tag="VF089_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6174182.1"
                     /translation="MYRTDQYEGMIAETMSMQGHKGEAINAYVARPLGAGPFPGMVLI
                     HHAPGWDEWYRECTRKFAHHGYVAISPNLYFREGHGSPEDVGAKVRAAGGVPDEQVLG
                     DLDGAKNLLRSLPYVSKVGVFGTCSGGRHGFLAACKLKGFDALVECWGGGVVMKQEEL
                     TPKRPVAPIDFTKDLSCPMIGIFGND"
BASE COUNT          978 a         1182 c         1100 g          902 t
ORIGIN      
        1 caagccttac gcatcaaccg ggcgcggcgg cgtggcgcga aactgatcgt catcgatccg
       61 cgcaaaacca cgctcgcggc aaaagccgat tgctggttgc gggttcgtcc tggaaccgac
      121 ggcgagctgg cgatggcgat gatccacgtt ctcatcgaag aaaagctttt cgacgaggag
      181 ttttcccgcc gttggaccaa cgcagcatgg ctggttcgct gcgataatca tcaacttctt
      241 accgaacgtg atcggcagat caatggaaac cccagcacat acctcgtctg ggacggatac
      301 gatgatgcac cggtcgcagt gggacgaaat actgctgcgg cgctgtttgg cagcttcagc
      361 gtggtcctcg gtgatggaac agaagttaac tgtcagaccg cgcttcaaat gctcaaagat
      421 agagccgccg gctacgcacc ggacctatcc gagggcatca cgacggttcc tgctgacgat
      481 gttagaaggg cggtgcgcct gttcgcgacc gaaaaaccat ccagctactg tacatgggtc
      541 gggctcgaac aagactgcga cgctatgcaa accaatcgcg ctgtctgctg cttttacgct
      601 ctgacggggc aattcgacga gcgcggcagt aacgttctct tcgccgccac accaaccact
      661 ccgatcaccg gccgcgagct actggcaaaa gataaagcgg atcgccggct cggaatcgca
      721 cagcatccgc tcggcccgcc cactgacccg ggacttgtcc aggctgcggc agtctatcag
      781 gccattctta ctgagcgtcc ctatccgatt aaggctctgg tattgttcgg cagtgatcca
      841 ctgctcggac acggcgatcc gctccacggc aaagcggcac tcgaagcggt tgatttctac
      901 atccacgttg acaccaccct taaccccagc gcaacattcg ctgatttagt gctgccggca
      961 accacgtgtt gggagcacga agccttgatg ccctctttcg aaatggccga ggacacactc
     1021 aattgggcgc agctccggcc ggcggtggca aagcctgtgg gcgagtcacg ctccgacatg
     1081 gcaattatct tcgacctggc tcagaggctc gggttagcgg aacagttttt caacggcgat
     1141 attgaagaag ccttcaagca tcaactggcg ccggcgggaa ttttcataga ggaactgcgc
     1201 gagaatccga ttggacttcg agccaacgtc agcaccaggc accgcaagta tgctgaaatt
     1261 gatccgcgca cgggcgaagc gaaaggtttc gaaacaccga cacggaagct tgaaatctat
     1321 tcaaccgtct tcgcgcaagc tggctatacg ccgctacccg aattccacac ccgagccgac
     1381 gatacagatc cgcgctatcc gctcactttg acgttttacc gcctggtaca attctgcgac
     1441 gaacaacacc gcaacattcc gcgcctgcgg cgatcgtttc ccgagccttt tgccgagctt
     1501 catccacaga cggctaaagg cgaacaaatc aaagatggtg aatggatttc actggaaact
     1561 gcaaacggaa aggtaaaact gaaggcgaag ttcaacggct ccttgcatcc tgcggtggta
     1621 gcgatggtgt acgggtggtg gcaagcctgc caactatcca atctcagcgg ttatgacccg
     1681 cttagtcaaa acggcgcgaa cgccaacctg ctcattgcaa actccgatgt cgatccgatc
     1741 agcgcatcgg ttgcgcaccg ctcgcagaga tgtcgcatcg ggaaactatc gggttagccg
     1801 ctgtttcaaa agtcgcagaa tacgcaatca atagtgcggt acttatttaa gtcagggcaa
     1861 tccttcattg atccgtcaat tcagcggcac aaacagttct tccagcgcca ttgttctcgc
     1921 catcaatcct tgatcgagcg aatagccaac gaagcgttcc aagttagggc ggttcttttc
     1981 gaggccgtac ggccagggat cgtctcccaa gagtttttgc tcttcttcaa acgcctgacg
     2041 tccccaagcc agccaggacc agttcgggtc ggcatagaac tcacggcaga tcttctttgc
     2101 ttcttggaac gtatcgaaaa ggctgcgcgc cgcttcagga tattttttta acacctcgtc
     2161 tttgaacgcg accacatgca tgatcgggaa aaagccgttt ttctgaaaat atctgagatc
     2221 ctccgccttc ggatcggcga ataggcggcg gatctttttc gagccgcgca gcaccggctt
     2281 gggcggatgg ggtgtcatca tggcgctgat ttcgcctgat tcgagcaggg cgccgatgtc
     2341 cgcgctatcc ggcagttgat cgatcctaac gctcttttcc ggctcgaatt ctatattgtc
     2401 cttcttgctg agcacccagt gaatttcccg ccaggggaca ttgaattcgc tttgcaagtc
     2461 gcctttggaa agtgtcgcca gagtattctg aaagctatgc acgccaactc tcttgccgat
     2521 cagatcacgc ggcgagtgaa ccgccgcatc cacgttgacc cacatttgtg aaagactaaa
     2581 caagcggcgc ggaaacacgg gaatggccgt gaacggcata gctcgactct tggccatgag
     2641 ataagaggat agagatagct cggctacgtc gaattcagaa cgctgcaaca tccgttcgtg
     2701 gcgatcttcg ccgtcccgtt gtcgatcgga ttgaccaacg attaagatgt tgagatcgat
     2761 cccgtccggc cgggccgtgc cgtcgaagaa aggcacatga cggtcgtaat gagacagcgc
     2821 gatggttagt tttggcttag gcataaggtt ctcaggagga gctaaaggca aaggaaatta
     2881 aaatggccaa aatagaaaga gcgccccggc ctggcgccct ttcctgatgt gatgatttac
     2941 aaattaccag cgattccgac caccgccgcc accgcgtctc tcgccacccc caccaccacc
     3001 gccgccaccg ccaccaaagc caccgccgga gcgtctttcc tggggacgcg cttcattgac
     3061 cgttaacgtc cgcccaccga gatcgctgcc gttaagcgct tggattgcct tctgagcctc
     3121 gtctgaggaa ctcatctcaa cgaatccgaa accccgcgaa cgaccgctga acttgtcggt
     3181 gatcacgcga gccgattcaa cagctccatg ggcggaaaaa atttcctgca gctgatcgtc
     3241 ggtagtagag tatggcaatc ccccgacata gatctcacta ctcatgttct cctcctctga
     3301 aaacgttgtt gttgtctttg accctgagaa cctgatcaac gcaggaatca gaacatgaaa
     3361 tggaaaacac gctctggccg gattgtccgc gaggtaagaa tcgcaaggaa agactgacca
     3421 agccttcgac tctcttattt gtcactacag aaggctccat actgaagtgt gcttttagcc
     3481 tactgggttt ccaaatccaa aacaagagga attttttacc gctttagttg cgtcaaagag
     3541 actttttgta ttaagtggac ggctgagcaa ttttcaagaa tattcgactt gaaggaggac
     3601 actatgtacc gaacggatca atatgaaggc atgatcgccg agaccatgag catgcagggc
     3661 cacaaaggcg aagccatcaa cgcttatgtc gcccgcccct taggcgcggg tccttttcca
     3721 ggcatggttc tgatccatca tgcgccggga tgggacgagt ggtatcgcga atgcactcgc
     3781 aaattcgccc accatggcta cgtcgcgatc tcaccgaatc tctacttccg cgaaggtcat
     3841 ggcagcccgg aagatgtagg cgcgaaagta cgcgccgcgg gcggagttcc cgacgagcag
     3901 gtcctgggag acttagatgg cgcgaaaaat ctgcttcgtt cacttcccta tgtgagcaag
     3961 gtaggcgtat tcggcacctg ctccggcggc cggcacggct ttctcgcggc gtgcaaactc
     4021 aaaggtttcg atgctctggt ggaatgctgg ggtggcgggg tggttatgaa acaagaagaa
     4081 ctcactccta aacgtcctgt agcgcccatc gattttacca aagatctttc ctgcccaatg
     4141 atcggcatct tcggcaacga cg
//
LOCUS       DASUTG010000402         5500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1397337, whole genome shotgun sequence.
ACCESSION   DASUTG010000402 DASUTG010000000
VERSION     DASUTG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397337"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VF089_09220"
     CDS             <1..1009
                     /locus_tag="VF089_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6174183.1"
                     /translation="PEGLDTIEVYPNGISTSLPLDIQVDLVHSIEGLEKAAIMRPGYA
                     IEYDYVEPTQLKPSLETKLIRGLYHAGQINGTTGYEEAAAQGLMAGINASLSNRGEAP
                     LILGRDQAYIGVLVDDLVTKGTDAEPYRMFTSRAEYRLMLREDNADFRLTEIGYRLGA
                     VSQVAHERLEQKKRDMATLLNLLNSFQLTPCRETNSVLAALGSAPIINKSSLAQLLRR
                     PELTLGDLIPLSPTIGDYPAHVATQLEAEIKYAGYLNRQSEMVNRSKQMETTRVPHDI
                     DYAAIGGLSREVREKLTRIRPLSLGQAARIPGITPAAISLLSVYLRRSFSRHELKASS
                     S"
     gene            1110..1913
                     /locus_tag="VF089_09225"
     CDS             1110..1913
                     /locus_tag="VF089_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174184.1"
                     /translation="MGKVIAVANQKGGVGKTTTSINLAASLSIEGKRTLLVDVDPQGN
                     STTGIGMNRSELVASLYDVLTGSHVLTQVTCETDLPLLFLVPASKDLVGAEIELVAAD
                     RREYRLKYSLAPVTDEYDYIVLDCPPSLGLLTLNSLTAADSLLIPIQSEYYALEGLSA
                     LLETMDLVKKGLNPHLQLQGIIITMFDSRNRLSHQVLEEIQKHFPDKLFKSIIRRNVR
                     LSESPSYGKPVCLYDPSSSGAQDYLDLARELIAHGDTHVTTENGIGQRS"
     gene            1876..2760
                     /locus_tag="VF089_09230"
     CDS             1876..2760
                     /locus_tag="VF089_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6174185.1"
                     /translation="MSLQKTGLGKGLSALIPVAPSQLVQSTPSDDPKTDVAIDKISPS
                     PFQPRRAFDEAKLQELAMSIRNQGIIQPLVVRPKGERFELIAGERRWRAAMKAGLIRV
                     PVVVREASDHDALQLALIENLQREDLNPIEEATGYRRLQEEFTWSQEEVAEKVGKSRP
                     AVANALRLLSLPSEVQQEVAAGNLPAGQARALLGLQSDAVIMTAYREVIARALSTRET
                     EKLVRNLKLGRRRRRDSPAVDPDLRSLVEDLQRALGTKIRVLPKARSDKGKIEIEYYS
                     LAELERIIGAIKRRSSSM"
     gene            complement(2802..4859)
                     /locus_tag="VF089_09235"
     CDS             complement(2802..4859)
                     /locus_tag="VF089_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6174186.1"
                     /translation="MDAKYTNRLSHETSPYLLQHAHNPVDWYPWSDEAFAKAKQENKP
                     VLLSIGYSACHWCHVMERESFENEQIAQLMNDLFINIKVDREERPDLDEIYMNAVQML
                     TGRGGWPMTMFLTPDRQPFYGGTYFPPEDRHGMPGFPRILQGVAQAYRDKPDDVQKSV
                     QQILSALKQMAHSDDHEHALGPEAIGKAAEQIASAYDPENGGFGQAPKFPNPGVYELF
                     LRQHHHSGTKRFLDMVTHTLTRMALGGIYDHLGGGFHRYSVDEKWLVPHFEKMLYDNA
                     QLVRTLALLYRATGNEFFRGVMEETLDYLIREMLHSEGGFYSTQDADSEGEEGKFFVW
                     SRTEVDQVLGEESGEIFCRIYDVTEAGNFEGKNILHPILTVPQAAGLFKKAPAEIEAL
                     VRQAKQKLFAVRDTRERPFRDEKIITSWNGLMLSGLAEAIKISAKPRFLAAGNRAIQL
                     IFTKMFSDGLLRHTYKDGKAKQLGFLDDYAFLAIGLVDFYEATLDRSALERAVNLTEV
                     MLREFWDSSQGGFFYTGNSHEQLISRTKPLFDASVPSGNAKTTELLLRLYHLTGKEDF
                     LRKAEIVLRSYIRLMESQPFGFAHMLCALDFYLRTPKDVVVLGEPGDSRTTELLKVIN
                     SLYLPSVTVQLVGPDEPPEEVSPLLAGKTQINGSATAYVCQNYTCSAPVTRPEELRLL
                     LVS"
     gene            complement(5048..5251)
                     /locus_tag="VF089_09240"
     CDS             complement(5048..5251)
                     /locus_tag="VF089_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX6174187.1"
                     /translation="MTGTIKKIIRPKGFGFITPDDGSDEVFFHRGSLAPRNQIEDFNE
                     GDTVQFQTRKGEKGPVAFDLKIR"
BASE COUNT         1393 a         1407 c         1391 g         1309 t
ORIGIN      
        1 acccgaaggc ttggatacga tagaggtcta tccgaatgga atatccacaa gcttaccctt
       61 agacattcaa gttgacctgg tacactcgat tgaggggctc gaaaaggcgg caattatgag
      121 gcctggctat gcgatcgagt atgactatgt tgaaccaact cagttaaagc cgtcattgga
      181 gacgaagctg attaggggac tatatcatgc cggacaaatc aatggaacca cgggctacga
      241 agaagcggcc gcccaggggc ttatggctgg aatcaatgca tctttaagca accgcggaga
      301 agctcctctg attttgggcc gagatcaagc atacatagga gtgctagtag acgatttggt
      361 gacaaaaggg acggacgccg agccgtacag gatgtttacc tcccgagctg aatatagact
      421 tatgttgcgt gaagacaatg cagatttcag attaacggag attggataca ggcttggtgc
      481 ggtcagccaa gtagcccacg agcggctaga gcaaaaaaag cgcgatatgg cgaccctttt
      541 gaacctcctc aacagctttc aactgactcc gtgccgtgaa acgaactcag tcttagcagc
      601 ccttgggtct gcaccgatta tcaacaaaag ctcgctagcg cagctgctcc gccgtccgga
      661 gctcacgctt ggtgatttga ttccgctctc tcctacgatt ggagactatc ccgctcatgt
      721 cgcaactcag ttggaagccg agatcaaata tgcgggatac ctgaatcggc aaagtgagat
      781 ggtgaaccgg tcaaaacaga tggaaacgac gcgggtgcct catgatatag actatgctgc
      841 tataggtgga ctctcacgag aagttcgaga gaagttaact cgcatccgcc cgctctcgct
      901 aggtcaagct gctagaattc ccggtatcac cccagcggcc atctcccttc tgtcagttta
      961 tttgaggaga agttttagcc gccacgagct gaaggcgtca tcgtcttaac cagagtttca
     1021 cgtgaaacaa gttctgcctt ttgttccgat ctgctttgtg gtacaactaa gccctactat
     1081 ctaagtagct aaatttcata ggatttcgct tgggaaaagt catcgcagta gccaatcaga
     1141 agggcggcgt gggtaagacc accacgtcga ttaacctggc cgcttcattg tcgatcgaag
     1201 gaaagagaac attgctggtc gatgtagacc ctcagggtaa cagcacaacc ggcataggta
     1261 tgaatcgctc tgagcttgtg gcgagccttt atgacgttct taccggaagc cacgtgttaa
     1321 cccaggtaac ttgtgaaaca gatttaccct tactgttttt agttccagca tcgaaagacc
     1381 tggttggtgc cgagatcgag ttagttgcag ccgaccgacg tgaatatcgt ctaaaatact
     1441 ccttagctcc cgtaaccgac gagtatgact acatagtgct tgactgccca ccgtctttag
     1501 gtcttttgac cctcaacagt ttgacggctg cggattcctt attgattcca atacaaagcg
     1561 aatattatgc ccttgaagga ctgagcgctc tccttgagac gatggacctg gtaaagaaag
     1621 gtttaaaccc tcatctgcaa ttgcaaggaa ttatcataac gatgtttgat agccgcaacc
     1681 ggctatccca tcaagttctc gaagagatcc agaagcattt tcccgacaag ctatttaagt
     1741 ctattatccg cagaaacgtg cgtttgagtg aaagcccgag ctatggtaag ccggtttgtt
     1801 tatacgatcc aagctctagc ggcgcccagg attaccttga tctagctaga gaactaatcg
     1861 ctcacggaga cacacatgtc actacagaaa acgggattgg gcaaaggtct tagcgccttg
     1921 atcccagtcg caccatccca gctggtgcag agcacgccaa gcgatgatcc caagacggac
     1981 gtagccattg ataaaatcag tccaagccca ttccagcccc gccgcgcgtt cgatgaagcc
     2041 aaacttcaag agttggcaat gtcgatccgt aatcaaggga tcattcaacc gctggtcgtc
     2101 cgaccaaagg gtgagcgatt cgagttgata gctggcgaac gccgctggcg tgcggcaatg
     2161 aaggcagggc tgattagggt gccggtagtt gtgagagagg caagcgacca tgatgcactt
     2221 cagcttgcac ttatagaaaa ccttcagcgt gaggacctaa atccgattga ggaagccact
     2281 ggttatcgcc gcctccagga agaatttacc tggagtcagg aagaagtggc ggagaaagtc
     2341 ggtaagagcc ggcctgccgt tgcgaatgcc ttgcggcttt tatcgcttcc ctctgaagtg
     2401 cagcaagaag tggccgctgg caacctgccc gcgggacaag cgagagcctt gctggggctg
     2461 cagagcgacg cagttatcat gaccgcctac cgtgaagtca tcgcgagagc gctttccaca
     2521 cgcgaaactg aaaaactggt acgcaactta aagcttggca ggcggcgccg gcgagactcg
     2581 ccagctgtgg atccggatct ccgttcccta gttgaagact tacaacgggc gctcggaacc
     2641 aagataagag tgttgccgaa ggcgcgaagc gataagggta aaatcgaaat agagtactac
     2701 agccttgccg agttggagcg aatcataggt gccatcaaaa gacgttccag ctccatgtaa
     2761 ccgactcgca ggtcagtgaa catcgtcacc gaaccaaatc attaacttac cagcaaaagc
     2821 ctaagttctt cgggccgagt tacgggcgct gagcacgtat aattctgaca tacataagca
     2881 gtggcgctac cattgatttg cgtttttcct gcgagcagtg gtgatacctc ctcgggaggc
     2941 tcgtcgggtc cgacgagttg cacggtcaca ctgggaaggt agagcgaatt gatcaccttg
     3001 agcaactcgg tggttcttga atcacccggt tcgcctagca caacaacgtc tttcggcgtt
     3061 cgtaagtaaa agtccaaagc acataacata tgggcaaaac caaagggctg actctccatt
     3121 aagcgtatgt acgagcgcag gactatttca gctttgcgga gaaaatcctc ctttcccgtt
     3181 aaatgataca gccgcagcaa cagttcggtc gttttggcat tgccggaagg aaccgatgca
     3241 tcgaagagcg gcttcgtgcg gctaattagt tgttcatggg agttcccggt ataaaaaaaa
     3301 cctccttggg acgagtccca aaactcccgc aacatgactt ccgtaaggtt aacggcccgt
     3361 tccagtgcag aacggtctag agtggcttca taaaaatcta caaggccaat ggctaagaag
     3421 gcataatcgt ccaggaaacc taattgtttt gccttcccgt ctttatacgt atggcgtagc
     3481 aagccatcgg aaaacatctt cgtgaagatc aattggatag cgcggtttcc tgccgccaag
     3541 aaccttggct tcgctgaaat cttgatagct tcagccagcc ctgagagcat taggccgttc
     3601 cacgatgtaa tgatcttctc gtcgcggaat ggtctttctc gtgtgtcgcg cactgcgaag
     3661 agcttctgct tcgcctgacg tactagcgct tcgatttcag ccggagcttt tttgaacaac
     3721 ccggccgcct gggggacagt caggatcgga tgaagaatgt ttttgccctc aaaattacct
     3781 gcttccgtta cgtcgtaaat gcggcagaaa atttcgccgc tctcttcgcc gagaacctgg
     3841 tcgacttctg tccgactcca gacgaaaaac ttaccttctt cgccttcgct atccgcatct
     3901 tgggtggagt agaatccccc ctcactatgc agcatctcgc ggatcaaata atccaaagtt
     3961 tcttccatca ctcccctgaa gaactcgttt ccggtagctc ggtagagcag cgccaaagtt
     4021 cgaaccagct gtgcgttatc gtaaagcatc ttctcgaaat gagggacgag ccacttttcg
     4081 tcgactgagt agcggtgaaa tccaccgccc aggtgatcgt agattccacc tagcgccatt
     4141 ctagttagag tatgtgtaac catgtcaaga aaacgctttg ttcctgaatg atgatgctgc
     4201 cgcaggaaca gttcgtaaac accagggttg ggaaactttg gtgcttgtcc gaagccgcca
     4261 ttctcgggat cgtaagcact cgcaatctgt tcggcagcct tgccaattgc ctccgggcca
     4321 agcgcatgtt catgatcgtc ggagtgagcc atctgtttga gagccgatag aatttgctga
     4381 acgctcttct gaacatcgtc gggtttgtct cggtaagcct gcgccacccc ttggagaata
     4441 cggggaaagc ccggcatgcc atgacgatct tccggcggaa agtatgtacc gccatagaat
     4501 ggctgccggt cgggagtaag aaacatggtc attggccaac ctcccctacc agtcagcatc
     4561 tgcacagcat tcatgtagat ctcatctagg tccgggcgtt cctcacgatc cactttgatg
     4621 ttgatgaaga gatcgttcat cagctgagcg atctgctcat tttcaaaaga ttcgcgctcc
     4681 atgacatgac accagtggca ggcggagtaa ccaatgctca gtagcactgg tttattctct
     4741 tgtttagcct tagcgaaggc ttcgtcactc cacgggtacc aatcgacggg attatgggcg
     4801 tgctggagca gataggggct ggtctcatga ctcaaacgat ttgtgtattt cgcatccact
     4861 tggactatct cccttccaaa tcaaacgcta acacatagta cgcggtctca aaaactaact
     4921 tcttgagccg cgaaaattca gtcgcagccg tccgtcagcg ctttagcctc gtccaggggt
     4981 ccaggtgatg atcagcacca tcagcaaaat agcaagaatg gttgaaggca gccgcgggaa
     5041 atctagctca ccggatcttt aggtcgaaag cgacgggccc tttctcgccc tttctggttt
     5101 ggaactgaac cgtgtcgcct tcgttgaagt cttcgatttg attacgaggt gccaggctgc
     5161 cgcggtgaaa gaaaacctca tcgctgccat catccggagt aatgaaccca aagccctttg
     5221 gtcggattat tttcttgata gtgccggtca tagcaatttc tcctcaagca cggtcaaagc
     5281 ggaacgcatt tcgctgcgat gtattgacaa tcaaatcaca tccaacttag cctacaaatt
     5341 aaaattcaca tggatcaagc tatcttgagt tcacttctct actatatcac cggccatggt
     5401 tacggccacg cagtccgttc ttgccaggtc atttggaatc tgaaagagcg ctgtccggaa
     5461 ctgacggtcc acgttcgcag taccgtcccg gaatggttgt
//
LOCUS       DASUTG010000403         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1989395, whole genome shotgun sequence.
ACCESSION   DASUTG010000403 DASUTG010000000
VERSION     DASUTG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989395"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF089_09245"
     CDS             <1..437
                     /locus_tag="VF089_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174188.1"
                     /translation="IESLQDFPGALLIVTHNERILRALATKLVVFHRGRVDVFNSGYD
                     EFLDKIGWEDETGGSSAQKKPAARINYNEKKEREKAERREKARIAKLEAQIVKSENAL
                     KEYHEQLMIEANRNNLAQMNDLSKKISQRTQEVDNLYREYAG"
     gene            complement(462..1613)
                     /locus_tag="VF089_09250"
     CDS             complement(462..1613)
                     /locus_tag="VF089_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174189.1"
                     /translation="MTAPTLDEIDRIARLSNPILRNLQITQCYYEISQAVAGFTGNSA
                     NWCTFATWASKQAGQTIRQEDLIRSFEERFYLSDKISAALEGIVSNLRRIGVRLHTEV
                     PRKAMLRAINPGAAFARASNAVARGNKKVFEEIGREFARFLGIFGEQTHLEPEKTARF
                     WAGLRAGEPPEGQRLLSQALTSYCEARFQSGQDKAELILLANLSAGFHEQIRLQPEIA
                     DALNAALDGVVESKRNLMTVLLPDFYVRAREKVLRDQTLYLDAAVGRLIEEVNRLIRQ
                     VITDHLMTLHLAGTEVLRLGRDLQGTFPQQLGQIRNPRLRELLERVDITPNSLDQTGA
                     KDWADLIDRMHFIADFFRVYQDRQELFEAPFTTDQVDRLKSGVKSSGRL"
     gene            complement(1665..2636)
                     /locus_tag="VF089_09255"
     CDS             complement(1665..2636)
                     /locus_tag="VF089_09255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174190.1"
                     /translation="MLAPVCVCFSVTVYLSETWAQPVKLTVSYTGVGPVNLPVVLAKE
                     TGIFARNGLDVTVVRAQAAVSTMALISGEVEFIQAAAPAVLQSNLGGSGAVYVAGGYT
                     GLDYWLVGHPSITSAEQLKGVIIGTAGLSGGSYTATHLAVKKLGLNPAKDVSIIAVGE
                     TPERLAALRTGRVQATSLNPPTIFAAEKEGFRILADVSALPFQNVAPVTTRRFIKENP
                     DVVRRYVKSQVEAVHVMKRDRKSGMRIFVQLMGGFKDTAVLEKSYDVSVTDDKLPRNQ
                     YPSLEGIKAVLNSLGDRSKDARPTDFVDMRFIKELEESGFIQALYKE"
     gene            3103..3939
                     /locus_tag="VF089_09260"
     CDS             3103..3939
                     /locus_tag="VF089_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6174191.1"
                     /translation="MINTVEYEVVLRDVEYQRQAGKPWLARIYQPKGAGPFPTIVDVH
                     GGAWHNGDRTNNAGIDRSLAAKGILVAAVDFRQPPEAGYPASICDVNFAVRWLKAHAD
                     EFNGTTAVGAFGNSSGGHQVVLSALRPRHPAYSVLPLPNYPELDARLTYVIAGWPVID
                     PLYRFRFAKESNREEHIKAHIAYWITEDAMAEGSPQTIVEQNEQAELPPILMLLKNND
                     RNHPLEMQERFIASYRKRGGEIEVHTFDGLPEHRVVPSPSQPDTMRLIDTITEFISRK
                     AR"
BASE COUNT          948 a         1133 c         1092 g          898 t
ORIGIN      
        1 taatcgagag cttacaagat tttccaggcg cattattgat cgtcacccat aacgaacgga
       61 ttcttcgcgc cctggccacg aaattggtcg tctttcaccg cggcagggtt gatgtcttca
      121 actccggtta tgacgaattc ctagacaaga tcggctggga agacgaaacc ggcggcagca
      181 gcgcacagaa gaagccagcc gcgcgaatca attacaacga gaaaaaagaa cgcgaaaaag
      241 ccgaacgcag ggaaaaggca agaatcgcca aactagaagc ccagatcgtg aaaagcgaaa
      301 acgcattaaa agagtatcac gagcaactga tgatagaagc caatcgtaac aatctggctc
      361 agatgaacga cctgtcgaag aaaatcagcc agagaacgca ggaagtcgat aacttgtatc
      421 gcgagtatgc gggataggtt cgtagcgtaa gcgattgacc gtcacagccg cccggaggat
      481 ttcacgcctg attttaaccg atcgacctgg tcagtcgtga atggcgcttc aaacagttcc
      541 tgccgatcct ggtaaacgcg gaagaaatcg gcgatgaagt gcatgcgatc gatcaagtca
      601 gcccaatctt tcgcaccggt ttggtccagg ctattcgggg tgatgtcgac ccgctcgagc
      661 agttctcgta gacggggatt gcgaatctga cccagctgct gaggaaatgt tccttgcaga
      721 tcgcgcccca gtcgaagcac ctccgtacca gctaaatgaa gcgtcatcag atgatcggta
      781 atgacttgtc gaatcagacg gttcacctct tcgattagcc ggccaactgc agcatcgaga
      841 tagagcgtct gatcccgcag caccttctca cgggcgcgaa cataaaaatc cggtagcagg
      901 acggtcatga gattgcgttt cgattcgacg acaccgtcga gcgctgcatt gagtgcgtca
      961 gcaatttccg gctgaagccg aatctgctcg tgaaagccgg ccgaaagatt ggccagcagt
     1021 atcagctcgg ccttgtcttg cccgctctga aaccgcgcct cgcagtagct cgtgagggcc
     1081 tggctcagga gtcgttgtcc ctccggcggc tctccggcac gcaacccggc ccagaaccgc
     1141 gccgtcttct ccggctcaag atgggtctgt tcgccaaaga ttccgagaaa ccgagcgaac
     1201 tcgcggccaa tttcctcgaa gactttcttg ttgccgcggg ctacggcgtt gctggcgcgc
     1261 gcgaatgctg cgccaggatt gatcgcccga agcatcgctt tgcgcggcac ttcagtgtgc
     1321 aacctcacgc cgatgcgcct gagattgctc acaatcccct ccagagcggc tgaaattttg
     1381 tcggagagat aaaagcgttc ctcaaagctt cggatcaaat cttcctggcg aatggtttgt
     1441 ccggcttgct tggaggccca tgtcgcaaac gtgcaccagt tggcggagtt gccggtgaat
     1501 ccggcgacag cctgagagat ttcgtagtag cactgggtaa tctgtagatt gcgcaggata
     1561 ggattgctga gccgggcgat tcgatctatt tcgtcaaggg tcggcgcggt cataggaaca
     1621 gcttcgagct taaggcgaaa ggtattcccc gaaatcgtta ggtatcactc tttatacaag
     1681 gcttggatga atccgctttc ttcgagctcc ttgatgaacc gcatatctac aaagtccgtc
     1741 gggcgcgcgt ctttgcttct gtcgccgagc gaattcaaga ccgccttgat gccctccaaa
     1801 gaaggatatt ggtttcgtgg cagtttatcg tccgtaaccg acacgtcgta acttttctcg
     1861 agcacagcag tgtccttaaa gccgcccatc agctgcacga aaattctcat gcccgatttt
     1921 ctgtcgcgct tcatgacatg caccgcttct acttgcgact taacgtagcg gcgcacgaca
     1981 tccgggttct ccttgatgaa tcgtctagtt gtcacgggcg ctacgttttg aaagggcaaa
     2041 gcgctcacgt ccgctaaaat tctgaagcct tctttttccg cggcaaaaat cgtcggtggg
     2101 ttgagagagg tggcttgaac tcgccctgtg cgcaaagccg ccaagcgctc cggggtctcg
     2161 ccgaccgcga tgatcgaaac gtctttggcg ggattgagtc cgagcttttt aacggccaag
     2221 tgggttgcag tgtacgaacc ccctgaaagc cccgcagttc cgataatcac ccctttaagc
     2281 tgttcggctg acgttatact tggatgaccc actagccagt aatcgagacc agtgtaaccg
     2341 ccggcgacat aaacagctcc tgagccgccg agattgctct gcagaacggc gggcgctgca
     2401 gcttgaataa attccacttc accggaaatg agggccatcg ttgacacagc ggcttgagct
     2461 cgaacaactg taacatcgag accgttcctg gcaaaaattc cggtctcctt cgccaaaaca
     2521 accggcagat tgaccggtcc aacgccggta taactcacgg tcagtttcac cggttgcgcc
     2581 caggtttccg aaagatacac cgtgacgctg aagcatacgc aaaccggtgc caacatcaga
     2641 cgtatcattt tcaaaatgct ctgtgaggta tcagtcacct ttcgttgcca tccctctctc
     2701 tcggatcttt cgcttgaccg gaggtacctg caaggttatt ccagaatacc ggaaactagc
     2761 gcaaacaaat tataacacca atagcgactg ttagccgcgc acaatggtgc tcttccacgc
     2821 actgcttatg atccaatccc cggagttatg agacagcctg tcggggctag ctcgctccac
     2881 agcagtgtta cgaaaaagcc atttgtataa cagagacgcc gttcgccact aactcgcctt
     2941 gatcatttcc tgcgccaatt tcattgatga aagagtgaga gggagtcctt ttcccggcgg
     3001 tttctcccga gctttctcgg atcatcacaa tgggtcgatt gtactagcct gtcgaatgtg
     3061 cctgacttgc agcgccggcc catacggctg tagattgcca ccatgattaa cacagttgaa
     3121 tacgaagtag tcctgcggga tgtcgagtat cagcgccagg ccggcaagcc gtggctggct
     3181 cggatctatc agcccaaagg ggctggacca tttccgacga tagtcgatgt gcacggcggc
     3241 gcatggcata acggcgaccg gaccaacaat gctggcatcg acaggtcgct ggcggcgaag
     3301 ggcatcttgg tggccgccgt cgatttccgc caaccgccgg aagcgggcta tcccgcttct
     3361 atctgcgacg tgaactttgc tgttcgctgg ctcaaagcgc atgcggatga gttcaatgga
     3421 accaccgccg tgggagcgtt tggcaattcc agcggcggtc accaagtcgt gctcagcgcg
     3481 cttcggccgc ggcacccggc gtattcggtg ctgccgctgc ccaactaccc tgaactcgat
     3541 gccaggctga cctatgtgat cgccggctgg ccggtgatag atccactcta tcgcttccgc
     3601 tttgccaaag agtccaaccg cgaagaacac atcaaggctc atatcgcata ttggatcaca
     3661 gaagatgcga tggccgaagg cagtccgcag acgattgtcg agcagaatga gcaggccgag
     3721 cttccgccga tactcatgtt gctgaagaac aatgaccgca atcatcccct tgaaatgcag
     3781 gagcgcttca tcgcgtccta tcgcaaacga ggcggagaga tcgaggtgca cacgttcgat
     3841 ggtcttccgg agcaccgtgt cgttccatcg ccgagtcagc cggacacgat gcgtttgatc
     3901 gataccatca cggagtttat ctctcgcaag gcgcgatgat gctgtctgag gtagtgaccc
     3961 gtctagtagg caactgttgc tgatcgcagc agaagctacg catcagctac ggcaaagctt
     4021 cggtgatctt ttcctttctg gcagtggccg acaggcattg tatcctatcg g
//
LOCUS       DASUTG010000404         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_63366, whole genome shotgun sequence.
ACCESSION   DASUTG010000404 DASUTG010000000
VERSION     DASUTG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63366"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1272)
                     /locus_tag="VF089_09265"
     CDS             complement(157..1272)
                     /locus_tag="VF089_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6174192.1"
                     /translation="MTSRKQIAALQSCISNPIDPSLIGYKQIAGIVGDGPSFYSKSPA
                     LWNAAFHSLGINAIYLPFDVAEPRLGELVATLKGLDSFLGINVTVPHKIRIMDFLDEI
                     EAGAKRVQAVNTVVRTSSGKLVGYNTDGEGFIESLLTPLPGETGSLVTSLAKSDVLLL
                     GAGGSARSVAFHVSDRLDEGQLVIANRSLDHAVSLASDIQKNGRKVKAIDECEVGDWA
                     RRVGLIVNSTTKGQGGIRKLPNGKLTTLEPYSALACAHPKSISQSDFDDPQTRQNWLC
                     AAQADIDANNQTSMMMAQTIPQKVRFYDLIYHPEETVFLRHGRLSGHLTKNGKAMIVY
                     QAAIALCRHICKQHLVGAGKDEAATYRAVCQVMTANW"
     gene            complement(1340..>2476)
                     /locus_tag="VF089_09270"
     CDS             complement(1340..>2476)
                     /locus_tag="VF089_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX6174193.1"
                     /translation="LQVLTIAHVYGHNDFFKNNFTFGATRAQYTIETFKSHANRVRHY
                     VEDPSIGLERIERILDAAHALSLQCRRNLSIRKLSSEEQIESKVQEAQPPADPFGAIH
                     RRQDYVSPDLNKVPLYPEEDLLIFVRDHNPHLADWEKDLLTIVHEQSQYFIPQIETKI
                     MNEGWASFWHKRILETLELPQNLHLEFLVRHAQVLRPTPGGLNPYHVGMKIWENIEKR
                     WDHPSAEDEKEFGRRTKSGAEKLFEVREVERDASFIRRYLTEDLVRDLNLFEYRTRGN
                     EQVVSRVADDLNWRQIKETLIQNVGTSTIPVIKIEDADYNKSRTLFLRHHHDGRDLQL
                     EYAEKTLQYLNQLWGHEVALETILDSRKSLLCYSDNRLAIKNGI"
BASE COUNT          567 a          637 c          613 g          659 t
ORIGIN      
        1 tggattgatc atgttggtat cccgccgggt gcggcttgcg tattgaaagt tgtcggagca
       61 ggtagttttc aacagcgcag ctagagagaa actccccggt ctcgttctag ctcatgcatg
      121 tttgtcggcg taattggatt cacgatcctt tttcttctac caattagcgg tcatcacctg
      181 gcaaaccgct cgataagtag cggcctcgtc cttgccagcg ccaaccaagt gctgtttgca
      241 gatatgtctg cacaacgcaa tcgcagcctg ataaacgatc atcgccttac catttttggt
      301 caggtggccg ctcaatctgc cgtgacggag aaataccgtc tcttccggat gatagattaa
      361 atcgtaaaat cgaacctttt gcggtatcgt ttgagccatc atcatggacg tttggttatt
      421 tgcatcgata tcggcctgcg ctgcgcaaag ccaattctgc cgggtttgtg ggtcatcaaa
      481 atcggattga gagatcgatt tcgggtgagc gcatgccaaa gcggagtatg gttccaatgt
      541 ggtaagcttg ccgttcggca acttacgaat tcctccttgt cccttggtgg tcgagttaac
      601 gatcaagccg actctacgcg cccaatctcc gacttcacat tcgtcgatag ctttaacttt
      661 ccggccattt ttttgtatat ctgatgccag ggacacggca tgatccaggc tacggttggc
      721 gattactagc tggccttcat cgagtctatc cgacacgtga aaggcgacgg accgtgccga
      781 gccgccggcc cccaagagca agacatcgct tttcgccagc gaagtcacga gtgatccagt
      841 ctccccaggc agcggcgtca acaaactttc aatgaacccc tccccgtcgg tattatatcc
      901 gaccagcttg ccggaggatg ttctgacaac ggtgttgacc gcttgaaccc gtttggcacc
      961 ggcttcaatc tcgtccagaa aatccatgat gcggatcttg tgcggcacgg tgacattgat
     1021 ccctaaaaag ctatccaacc ctttcaatgt cgcaactagt tctcctaggc gaggctcagc
     1081 gacatcaaaa ggtagataga tggcgttaat gccgagacta tggaatgccg cattccatag
     1141 ggcagggctc tttgaataga acgatggccc atcgccgacg attccggcaa tctgtttata
     1201 gccaattagg ctgggatcga tcgggttgct aatgcaactt tgaagggcag cgatctgttt
     1261 ccgggatgtc atggcaagta gctcgttgcc ccgtggtcac actcagcagt gattccgcag
     1321 gtaaggtttg tcggatcagc tatatgccgt ttttgatcgc cagtctatta tcggaatagc
     1381 acagcagcga cttgcggcta tcgagaatcg tctccaaagc gacttcgtgt ccccacaact
     1441 ggtttaaata ctgaagcgtt ttttctgcat attccagttg cagatctcgt ccgtcatgat
     1501 ggtgcctgag gaacagagtc cgactcttgt tataatcggc atcttcaatc ttgatcactg
     1561 gaatggtgct ggtgccgaca ttttggatca aagtctcctt gatctgacgc cagttaagat
     1621 catccgccac tcggctgacc acctgttcgt tgccccgtgt tcgatactca aacaagttca
     1681 ggtctcgtac gagatcttca gttaagtagc ggcgtataaa ggaggcatca cgctcaactt
     1741 ctcgcacctc gaagagtttt tccgcgccgc ttttagtccg gcggccgaac tctttctcat
     1801 cctcggcaga ggggtggtcc cagcgttttt cgatattctc ccatatcttc attcccacgt
     1861 gatacggatt taagccaccg ggggttggcc gcaacacctg cgcatgtcgc accaaaaact
     1921 cgagatgtag gttctggggc agctccaaag tttcaagaat tcgtttgtgc caaaagctcg
     1981 cccaaccttc attcatgatt ttagtttcga tctgcgggat aaaatactga gattgctcat
     2041 gcacgatcgt taaaagatcc ttttcccaat cagccaaatg cggattatgg tcgcgaacaa
     2101 aaatcaaaag atcttcctcc gggtaaagag gaactttgtt gagatccgga gaaacataat
     2161 cttgccgccg atggatagcg ccaaatgggt ccgcgggcgg ttgcgcctcc tgcactttgc
     2221 tttctatttg ttcctcggag gacagcttac gaattgatag attgcggcgg cactgaagcg
     2281 acaatgcgtg tgccgcgtca agaatacgtt caattcgttc caatccgatg ctcggatcct
     2341 ccacatagtg gcgcacccga ttcgcatgac ttttgaaggt ttcaatcgta tattgtgcgc
     2401 gcgtggcgcc aaacgtaaaa ttgtttttaa aaaaatcgtt gtgtccgtat acgtgggcga
     2461 tggtgaggac ctgcaa
//
LOCUS       DASUTG010000405         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1248206, whole genome shotgun sequence.
ACCESSION   DASUTG010000405 DASUTG010000000
VERSION     DASUTG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248206"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF089_09275"
     CDS             <1..884
                     /locus_tag="VF089_09275"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX6174194.1"
                     /translation="ALLTDERTGYYRDYGEFRQLVKAYTEGYVYSGEYSKYRDRRHGS
                     STRDIPAERFVVFSQNHDQVGNRALGRRLTELVSFEKLKLAAGAVLLSPFIPLVFMGE
                     EYGEEARFQYFISHSDSELIEAVRKGRREEFAAFEWQFEPPDAQDEATFRRAKLNHQL
                     RQDGRHRLLREFYGELIRLRKTLRPLARLSKAHCEISAYEKQHVMLMRRWKGDQANLI
                     FYNFSNAEVSLAAAIPAGDWQKIFDSAAPEWDGPGARLPEAFQGGKEWSLSMSAWGFA
                     LFHAGPIEYNGKEANKL"
     gene            881..3040
                     /gene="glgX"
                     /locus_tag="VF089_09280"
     CDS             881..3040
                     /gene="glgX"
                     /locus_tag="VF089_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006788783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6174195.1"
                     /translation="MKLWPGKPYPLGALWDGAGVNFSLFSENAERVELCLFDSSEGKE
                     SARIPLREKTAHVWHIYLPEVRLGQLYGYRVHGPYEPEQGHRFNPAKLLLDPYAMAIA
                     GTIKWGDELFGYPIGHSDADLAANERDSSDRLPKCVVVDPAFSWGNDVAPRTPWHKTL
                     IYELHVKGFTARHPGVPERLRGTYAGLACPAVLEYFHSLGITAVELMPVHQFVADRQL
                     VDRGLTNFWGYNSIGFFAPDARYSSSGVLGQQVAEFKTLVKTLHREGIEVILDVVYNH
                     TAEGNELGPTLSFRGIDNASYYRLVENDRRYYMDYTGCGNTLNATHPAVLRLIMDSLR
                     YWIQEMHVDGFRFDLASALARELHAVDRLGAFFDIIHQDPVISQVKLIAEPWDLGEGG
                     YQVGNFPALWAEWNGIYRDTVRRFWKGDSGQVGSMAYRLTGSSDLYGTSDRQPHASIN
                     FITAHDGFTLHDLVSYNEKHNEANGEDNRDGHNDNLSWNCGAEGPTDDIAIIELRERQ
                     KRNFLATLLLSQGVPMLLAGDEIGRSQSGNNNSYCQDNEISWVDWNLDPRRQSLLQFT
                     RGLIQLFHDHPVLRRRHFFQGRSIRGTAVKDLSWFRCDGKEMTEEDWNNAENRCVGLI
                     LSGDAVDELDARGNRITDDTFLFLLNAYHQRVAFTLPGPSAKAQWQVVLDTREGMARR
                     TRRVSKGANYNLEGRSLALFRLFDSQEALPMVLARRR"
     gene            3053..>5397
                     /gene="treY"
                     /locus_tag="VF089_09285"
     CDS             3053..>5397
                     /gene="treY"
                     /locus_tag="VF089_09285"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX6174196.1"
                     /translation="MRIPNATYRLQFHRSFTFADAKSIVSYLGDLGISDIYASPIFRA
                     RAGSLHGYDVVDPNQINPELGGAQEFDGLIGEIQRHCMGWLQDIVPNHMAYDSENWML
                     MDVLENGLNSAYANYFDIEWDHPMENMHHKVLAPCLGKFYGQCLEDGEIVLKYDAQGL
                     AVHYFQLRLPINIECYSMVLTPSLGALRRKLGRHNPDLIKLLGVLYTLKNLPSSEEAR
                     ERADQIIFVKNMLWELYTTNDEIRAFLDENIARFNGRPGAPETFNPLDQLLAQQYYRL
                     SFWKVATEEINYRRFFNINELISLRVEDPNVFELCHRLIFQLIREGKITGLRIDHIDG
                     LYDPAVYLRRIRQSLGEVYVVVEKILDMREDLPNDWLVCGTTGYEFANFVNQLFCDSD
                     HEQKFTELYRRFTGHNAAFADLVSEKKRLIIGRYMAGDVDRLAHLLKRVSSRDRFGGD
                     ITLYGLKRALVEVLTFFPVYRSYISHEKFGAEDRGRMQETLGKAKEMNPGLILELSFI
                     GKFLMLENADQLSEQEKSQWIDFIMRLQQLTGPLMAKGFEDTTLYIYNRFLSLNEVGG
                     DPSQFGLGPNAFHEFNLRRARSWPHSLNATSTHDTKRGEDVRARLNVLSELPDEWERH
                     VFEWGEMNRPHKRLVRGREVPDRNDEYFLYQSLIGALPLAAEEHAGFVDRIKNYTIKA
                     VREAKVHTGWLKPDTDYEDTFLGFIERILEPSKDNAFLAQLRQWTGKIARFGALNSLS
                     QTLLKLTCPGAPDLYQGSELWDLHFVDPDNRQAVDFECRQKL"
BASE COUNT         1231 a         1457 c         1520 g         1189 t
ORIGIN      
        1 gcgcgctctt gactgacgag agaaccggtt actatcgcga ctacggagag tttcgccagc
       61 tggtcaaagc ctataccgaa ggttacgttt actccggcga gtattcaaaa taccgtgacc
      121 gcaggcacgg cagctcaacc cgggatatac cggcagagcg cttcgtggtc ttttcgcaaa
      181 accacgatca ggttggcaac cgcgcgctag gcaggcgcct gactgagctg gtctcgttcg
      241 agaagctcaa gttggcagcc ggagcggtat tactgtcgcc tttcattccg ctcgttttca
      301 tgggtgaaga atatggtgaa gaagcgcgat tccaatattt tataagccat tcggattctg
      361 agctcatcga agcggtgcgg aagggtcgga gggaagaatt cgccgctttt gaatggcaat
      421 ttgagccgcc ggatgcgcaa gacgaagcga ctttccgccg cgccaagtta aatcatcaac
      481 tgcgccaaga tggccggcac cgactcttgc gagaattcta tggcgagctg atacgcttgc
      541 gtaaaaccct tcggccactg gcgcgtttga gcaaagcgca ctgtgaaatc agcgcgtacg
      601 agaagcagca cgtgatgttg atgcgccgct ggaagggcga ccaagccaat ctgatctttt
      661 ataatttcag taatgccgaa gtttcgctcg cagcggcaat tcctgcagga gattggcaaa
      721 agattttcga ttccgcggcg ccggagtggg acggcccggg cgctcgtcta ccggaagctt
      781 ttcaaggcgg gaaagaatgg tcgctgtcga tgagcgcatg gggattcgcg ctctttcacg
      841 ccggcccgat cgagtacaac gggaaggaag caaacaaatt atgaaactct ggccgggcaa
      901 accttacccg ctcggggcgc tctgggatgg cgccggcgta aatttttctc tcttctccga
      961 gaacgccgag agggtcgagc tttgtttgtt cgattcttcc gagggtaaag agagcgcgcg
     1021 gattccgcta agagaaaaaa ccgcgcacgt gtggcacatc tatcttcctg aagttcgcct
     1081 tgggcagctt tatggttatc gcgtgcacgg tccctacgaa cccgaacaag ggcaccgctt
     1141 caatccggcg aagctgcttt tggaccccta tgccatggcg attgccggaa cgattaagtg
     1201 gggcgatgag ctgtttggct atcccatcgg tcattctgac gccgatctgg cggcgaacga
     1261 gcgcgacagc agcgaccgcc tgcccaagtg tgttgtcgta gatccggcgt ttagttgggg
     1321 caacgacgtg gcgccgcgta caccatggca caagacattg atttacgagc ttcacgtcaa
     1381 aggctttacc gcgcgccatc ccggagtgcc ggaacggcta cgcggcacct atgcggggct
     1441 ggcctgcccg gcagtgctcg aatattttca ttctttgggg attactgccg tagagttgat
     1501 gccggtgcat caatttgtcg ccgaccggca gctggtcgat cgcggtctca ccaacttctg
     1561 gggctacaat tccatcggct tctttgcccc tgatgcgcgc tattcgagca gcggcgtgct
     1621 cggacagcag gtggcggaat tcaaaactct ggtaaagacg cttcaccgcg aagggatcga
     1681 ggtcatactc gacgtcgtct acaatcatac cgccgaaggc aatgagctgg ggccaaccct
     1741 gtcgtttcgc ggcatcgaca atgcgtcgta ctatcgactg gtggaaaatg atcggcgtta
     1801 ctacatggac tacactggct gcggcaacac gctcaatgcc acccatcccg cggtcctgcg
     1861 cttgatcatg gacagtttgc gttattggat tcaggagatg cacgtcgatg gtttccgttt
     1921 cgatttggcc tccgctctgg cacgcgagct ccatgccgtg gaccggctcg gcgccttctt
     1981 cgatatcatt catcaggacc cggtgatctc acaggtcaaa ctcatcgccg aaccatggga
     2041 tctcggcgaa ggcggatacc aggtgggcaa ctttccggct ctctgggccg aatggaacgg
     2101 catctaccgc gataccgtgc gccgcttctg gaagggcgac agcgggcaag tcggcagcat
     2161 ggcgtatcgc ctgaccggca gcagcgactt gtacggcacc agcgatcgtc agccgcacgc
     2221 cagcatcaat ttcattaccg cgcacgatgg gtttactttg cacgacctgg tcagttacaa
     2281 cgaaaaacac aacgaagcca acggcgaaga caatcgcgat ggccacaacg acaacttaag
     2341 ctggaattgc ggcgcggaag gccccaccga cgacattgca atcatcgagc taagagagcg
     2401 gcagaaaaga aactttttgg ccactctgct gttgtcccaa ggcgtgccga tgcttcttgc
     2461 tggcgatgaa ataggccgct cgcaaagtgg caataacaat agctactgcc aggacaatga
     2521 gatctcctgg gtcgattgga atctcgaccc gcgacggcaa tcgttgctgc aatttacccg
     2581 tgggttgatt cagttgtttc acgatcatcc ggtcttgcgg cggcgccatt tctttcaggg
     2641 gcgcagtatc cgtggtacag cagtcaagga tctgagctgg tttcgctgcg atggcaaaga
     2701 aatgacggaa gaagattgga acaacgcgga aaaccgctgt gtcggtttaa tcctttcagg
     2761 cgacgctgtc gacgaactcg acgctcgtgg caatcgaatc accgacgaca catttttgtt
     2821 cctgctgaac gcgtaccacc agcgggtcgc gttcacgctg ccgggaccca gcgccaaagc
     2881 acaatggcag gtagttctcg atacccggga ggggatggcg cggcggacgc ggcgcgtgag
     2941 caagggcgcc aactacaatc tggagggacg ttcgttggct ttgtttagat tattcgacag
     3001 ccaagaggcc ttacccatgg tgttggcgcg ccgacgctag taaaaagatt tcatgcgaat
     3061 tcccaacgcc acataccggt tacagtttca ccggagtttc actttcgccg acgcaaaatc
     3121 aattgtctcc tatctaggag atctcggtat ctcggatatc tacgcttcgc cgatttttcg
     3181 cgcccgggcc ggcagccttc atggctacga tgtcgtcgat ccgaaccaga tcaatcccga
     3241 gctgggtggc gcgcaggagt tcgacggatt aatcggcgaa atacagcgcc actgcatggg
     3301 ctggctccaa gatatcgttc ccaatcacat ggcgtacgac agcgagaact ggatgttgat
     3361 ggacgtgttg gagaatggac tgaattcggc ttacgccaat tatttcgaca tcgagtggga
     3421 tcacccgatg gagaacatgc atcacaaggt tctggctcct tgcctcggca aattctatgg
     3481 acaatgtctg gaagacggag agattgtcct gaagtatgac gcccagggac tggcggtgca
     3541 ttactttcag ttgcgcctgc cgatcaacat agaatgctac agcatggtcc tgacgccgag
     3601 cctcggcgct ttacgacgaa agttagggcg tcataacccc gatctgatca agcttctggg
     3661 tgttctttac acgctcaaaa atctgccgtc cagtgaagaa gcaagagaac gggcggatca
     3721 gatcattttc gtcaagaaca tgctctggga gctctacacg accaacgatg agataagggc
     3781 ctttctcgat gagaatatcg cgcgcttcaa cggacggccc ggcgctcccg agaccttcaa
     3841 ccctttggat cagctgctcg cgcaacagta ttatcgactc tcgttctgga aagtcgccac
     3901 ggaagagatt aattatcgcc gcttctttaa catcaatgag ctgatctcat tgagggtaga
     3961 ggatccgaac gtattcgagc tttgtcatcg gctcatcttc cagcttattc gcgagggcaa
     4021 gatcaccggg ctgcggatcg accacattga tggactctac gatccggccg tgtatctcag
     4081 gcgcatccgg caaagtctcg gagaagttta tgttgtcgtc gagaagattc tggatatgcg
     4141 cgaggacctg ccgaatgact ggctggtgtg cgggacgacc ggttatgaat tcgccaattt
     4201 cgtcaaccaa ctgttctgcg acagcgatca cgagcagaaa ttcaccgagt tataccggcg
     4261 cttcaccggt cacaacgcgg cgttcgctga tctcgtcagc gagaaaaaac gtctgatcat
     4321 cggccgctac atggccggag atgttgaccg cctggcgcat ttgctgaagc gggtttcgag
     4381 ccgggaccgt ttcggcgggg atatcaccct ttatggattg aagcgcgcgc tggtcgaggt
     4441 gttaacattc tttcccgttt atcgaagcta catcagccat gaaaagttcg gagctgagga
     4501 ccgaggccgc atgcaggaaa ctctgggcaa ggccaaggag atgaaccccg gattgattct
     4561 tgagctctcg tttatcggca aatttctcat gctcgaaaac gccgaccagt taagcgaaca
     4621 ggaaaagagc caatggatcg atttcatcat gcgcctgcag caattaaccg gaccgctgat
     4681 ggcgaaaggc ttcgaagata ccaccttgta catttacaac cgtttcctgt cgctcaacga
     4741 ggtcggcggc gatccgagcc agttcggcct gggcccgaat gcctttcatg aatttaatct
     4801 acgacgagcc cggagctggc cgcatagtct caacgcgact tcgacccacg ataccaagag
     4861 aggagaagac gtgcgcgccc gcctgaacgt gctgtccgaa ttgcctgatg agtgggagcg
     4921 ccatgttttc gaatggggcg agatgaatcg gccgcacaag cggctggtaa ggggacgtga
     4981 agttcctgat cgcaacgacg aatattttct ttaccagtct ctgatcggtg cgttgccgct
     5041 ggccgccgaa gaacatgccg ggttcgtcga tagaatcaaa aattacacga tcaaagcggt
     5101 gcgtgaagcc aaagtacaca ccggttggct caagcccgac accgactatg aagacacttt
     5161 cctcggcttc atcgaaagga ttctcgagcc gtcaaaagac aatgcctttc ttgcccaact
     5221 gcgccagtgg accggcaaga tcgcgcgatt tggcgcgctt aactctttat cgcagactct
     5281 tctgaagctt acttgccccg gcgcacccga tctctatcag ggaagcgagc tgtgggatct
     5341 tcacttcgtt gatccggata accgccaagc ggtggacttc gagtgccgac aaaagct
//
LOCUS       DASUTG010000406         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1843225, whole genome shotgun sequence.
ACCESSION   DASUTG010000406 DASUTG010000000
VERSION     DASUTG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843225"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..650)
                     /locus_tag="VF089_09290"
     CDS             complement(405..650)
                     /locus_tag="VF089_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174197.1"
                     /translation="MSLSKDSSVESAIKTILKNRCMSARLALVHCKFVYDYDAIANAI
                     SREKIASHDKNLWRYRKLLPSDGEHQVRMYSVITARI"
     gene            complement(678..1472)
                     /locus_tag="VF089_09295"
     CDS             complement(678..1472)
                     /locus_tag="VF089_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX6174198.1"
                     /translation="MSFSLTTEQIERYSRQILVPDFGGKSQIRLRQGRVLIIGAGGLG
                     SPTAFYLTAAGVGTLGLVDPDNVELSNLQRQILHATTDLGKAKVESSKETLTGLNPDV
                     EVITYPMRLDSDNVDGLFSEYQFIIDGSDNFETKFLVNDAAVRLRKAYSHAGIVRWQG
                     QTMTVISGKTACYRCLFKEPPPPAEILNCQQSGILGAVAGTIGSIQATEAIKYLIGFE
                     EGLLTDRLLTYDARAMKFHQVEVTKDPECKACRQLAMTEAAVSKTI"
     regulatory      complement(1531..1640)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1911..>2738
                     /locus_tag="VF089_09300"
     CDS             1911..>2738
                     /locus_tag="VF089_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6174199.1"
                     /translation="MGLTQEVANYVSKVSYKDIPSEVIRLACGFILDGLGVALAGSTE
                     NCSRIVQAHVREIGGKRECAVLGTALLAPAPQAALANGVAGHALDYDDTQLSTSGEAV
                     YGLLTHPTTPVLAAVLAVGESQKITGAEFLLAYIVGVEVECRIADSINPRHYQSGFHS
                     TATIGGLGAVMAVGKILRLEENVLVQALGICASMAAGLRENFGTMTKPLHAGRAAENG
                     VTAALLAQSGFTAATNILEAQRGFYQAMAGGYDESKIAGRLGRPYFMQEPGISIKPYP
                     "
BASE COUNT          592 a          725 c          740 g          681 t
ORIGIN      
        1 gaaatttagc cgccggccgc ctgtgagaac ggcggattca gatcttgatg gagctgatcg
       61 aaatgtttca acgcggcttt aatcgtgtag ggccgctcga ccttgctcac tcccgcctcg
      121 agagttttgt aaccgttacc ggtgatgcta ataacgatcg gttcatcgtg gggaattcgt
      181 cctttttgga tgagttcctg gttaccgaaa cttcggttcc tcccgccggc tcggtaagaa
      241 ttccttcagt acttgcgaga agcttgatcc cttcatcact tctttatctg tgacactttc
      301 accccaacgc ccgattcttt gatcgcgtcg cacgacatag aagctatctg ccggattgcc
      361 aatagcgatc gagctcgcaa tcgtgttggg ctttaccggg cttatcagat ccgtgccgtt
      421 ataaccgagt acatccgaac ctgatgttct ccatcgctcg gcagcagctt gcggtagcgc
      481 caaagatttt tgtcgtgtga cgcgatcttt tcgcggctga tcgcgttggc gattgcgtca
      541 taatcgtaaa caaatttgca gtggaccaaa gcaagtctcg cagacatgca gcggttcttg
      601 aggatagtct tgatcgcact ctcgacactg gagtcctttg acaaactcat gaagtttcct
      661 ttttctagcc cttactctta gatggttttt gaaaccgccg cctcagtcat agccaattgc
      721 cggcacgctt tgcactcggg gtcttttgtt acctctacct ggtgaaactt catggctctg
      781 gcgtcgtagg taagcagacg gtcggtcagc agtccttctt caaagccgat tagatacttg
      841 atggcctcgg ttgcttgaat cgagccaatg gttccggcga cggcaccaag gataccggat
      901 tgctggcaat tcaagatttc tgcgggtggc ggcggctctt tgaagaggca gcggtaacaa
      961 gcggttttgc cggaaattac agtcatggtt tggccctgcc atctgacgat gccggcgtgt
     1021 gaataagctt ttctcaggcg caccgcagca tcgttgacca ggaacttggt ctcaaaattg
     1081 tcgctgccat cgataataaa ttgatactct gaaaagagac cgtctacatt gtcggagtca
     1141 agcctcatag gataagtaat cacctcgaca tcgggattga gtccggtaag tgtttctttg
     1201 ctggattcta cttttgcctt gcccagatcc gtggtggcat gaagaatctg acgctgcagg
     1261 ttcgatagtt cgacattatc gggatcgacc aaccctaagg tgcctacccc ggctgcggtc
     1321 aggtaaaatg cagtaggcga gcccaagccg ccggcgccga tgatcagaac ccggccctga
     1381 cgcaggcgta tctggctttt gccgccgaag tccggcacaa gtatctgacg gctatagcgt
     1441 tcaatttgtt ctgttgtcag gctgaagctc ataaactctg tccaaaaaaa aacctcttct
     1501 gtcgataaga agaggttttc aaatcacctc accttatctc tcccccacgc cgcgggggct
     1561 ggaattagca ccagcgttcc tttcggaacc ggttgctgtg gcttcgaagg gcccgatccc
     1621 tccaccactc tgaataaggt tcagaataaa tttacacgtg aacgtatcgt aggcgattca
     1681 cggtgtcaag caatcaagcg agctgggccg ccggaatgtc caattcggta aacaaggatt
     1741 tcggcattgt atcccgatat gtaatcagcg aagcggttca gtgagtgatc gttatcgcac
     1801 ccaacctttg agtcaggctg ctgctttgaa aaagaaggtg aaaaaccgct tcagttctga
     1861 tatcagtctt ctccatttta caggtgaatc cgaccattaa aggagcttcg gtgggtttga
     1921 cacaagaagt agcgaattac gtctcgaagg tgagctacaa agatattcct tcggaagtga
     1981 ttcgcttggc gtgtggcttc atcctcgacg gactcggtgt ggcactggcg ggctcgaccg
     2041 agaactgctc gcgcattgtg caagcgcacg tccgtgagat aggtggtaag cgggagtgcg
     2101 ccgtgcttgg aacagccttg ttggcacccg cgccccaagc tgcgttagcc aacggcgttg
     2161 ccggtcatgc gctggactac gatgacacgc agctttccac gtccggtgag gcggtgtatg
     2221 gcttgctgac acatccgacg acaccggttc tggccgccgt tttggccgtc ggcgagagtc
     2281 aaaagatcac tggcgcagag ttcctgctgg catacatcgt cggagtcgaa gttgagtgtc
     2341 gcatcgccga ctcgatcaat ccgcgccatt atcagtcggg gtttcattcg accgccacta
     2401 tcggcgggct cggcgcggtg atggcggtgg ggaaaatttt gcgactggag gaaaatgttt
     2461 tagttcaggc tctcggtatc tgtgccagca tggccgccgg actgcgcgag aacttcggca
     2521 ccatgaccaa gccgcttcac gccggccgcg ctgcagagaa cggtgtaacg gcggccctgc
     2581 ttgctcagag cggtttcacc gccgccacca acattctcga ggcgcagcga gggttttatc
     2641 aagccatggc cggcggctac gatgagagca aaatcgccgg acggcttggc cgaccctatt
     2701 tcatgcaaga gccaggaatt tcgattaagc cctacccc
//
LOCUS       DASUTG010000407         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1248804, whole genome shotgun sequence.
ACCESSION   DASUTG010000407 DASUTG010000000
VERSION     DASUTG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248804"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF089_09305"
     CDS             complement(<1..206)
                     /locus_tag="VF089_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6174200.1"
                     /translation="MAEKTVARSGNPMANEMGQSKERTRGMERFVPPPVDIYDSPEGL
                     VLLADLPGVAPEDLKLRLEENTLT"
     gene            complement(244..705)
                     /locus_tag="VF089_09310"
     CDS             complement(244..705)
                     /locus_tag="VF089_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6174201.1"
                     /translation="MATWQSLDPFQALQREIENAFERVGPHFSPTLRSAFLPGRAARE
                     YPLINLYEEQNQVRVEALAPGAEPNSFNISMVGRTLTIAGEKPRASGEVKPEAFHREE
                     RAAGRFTRNIEIPFDVEQGEGLTGQYRHGLLVLTLPKSEKAKPRQITVQVG"
     gene            complement(1933..2400)
                     /locus_tag="VF089_09315"
     CDS             complement(1933..2400)
                     /locus_tag="VF089_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6174202.1"
                     /translation="MFGELMRFNPSEELSNWHRDIDDLFGRFFGHPETTSLGNWVPRM
                     ETYRKDNEYVLRFDLPGVNPNDVQVQAEGNVLSISGERKSEEKREGYQETSYGKFERT
                     VTLPQGIEADKIAARYEHGVLEIHVPLPAQLAGRKIPVQIEQKENKKLESKAA"
     gene            3320..>3647
                     /locus_tag="VF089_09320"
     CDS             3320..>3647
                     /locus_tag="VF089_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174203.1"
                     /translation="MIQSGAVQILSLGIDDIPRMRELMRKYRDLPMDLADAGLVRVAE
                     RERLRRTFTLDRRDFQVYRRHESVASPSFHLDRNFHTFNIKQNSVVSQDAGRAYEDRD
                     SLSISQA"
BASE COUNT          800 a         1000 c          963 g          884 t
ORIGIN      
        1 atagtcaggg tgttttcttc aagccgcagc tttagatctt ccggcgctac cccaggcaag
       61 tcggcgagca acaccagtcc ttcggggctg tcataaatgt ccaccggcgg cggcacgaat
      121 cgctccatgc cgcgcgtccg ctctttggat tggcccatct cgttggccat tggattaccg
      181 ctcctcgcga cagttttctc tgccatggta tctctcctcc ttagctaatt ccggcgactc
      241 gattcatccc acttggactg taatctgccg tggcttggct ttctcagatt tcggtaaggt
      301 cagcaccaac agtccatgtc ggtactggcc ggtgagacct tcgccttgtt cgacgtcgaa
      361 tggaatctca atgttcctag tgaatctgcc cgccgcccgc tcctcgcgat gaaacgcttc
      421 cggttttacc tcaccagagg cccgcggttt ttctccagcg atcgtaagcg ttctcccgac
      481 catgctgatg ttaaatgaat ttggttcggc ccctggggcc aaagcttcta cacgcacctg
      541 attttgttct tcgtacaaat taatcagcgg gtattcacga gctgcccgcc ccggtaaaaa
      601 cgcgctacgg agcgtcggtg agaagtgcgg cccgactcgc tcaaatgcat tttcgatttc
      661 tctttgcaga gcctggaatg gatcgagtga ttgccacgtc gccatttgcg tttcctccct
      721 ttcttttttg ttaggcctga tacagctcgc gcctgcggct agcgagcgca agcgaagtaa
      781 tgaatctctc cctaccggct aacgagatca gttttcctgg ccagcggttg tcaatccgac
      841 caggttagtg gaaccgaaat tggatgttgt cgctgtactg aaacagaatc gaatgcacta
      901 gcaggctgct gaaaagaccc atatgcttcg ttgccgctca attgcctcgc tgcgacgtac
      961 cgtaagtacg cctccgctcg tcgatttttc gcgcgcctcg catctggatc tttttgagca
     1021 gcctgcaata ggcttttttc agcaccctgc tggcgatcat ccgataattg cgcaactgtc
     1081 gtgccagagg gaccgcgtgt agaggagcag cacagaactg cgttggagta aaaagtacga
     1141 cccggaacaa gggtcgacgt tttccgtgat tgttttagtt gttaccccgg ccgaaaaata
     1201 ctcgttgcta tttgaaccgt tggcgcggtc tgcaccgttg ggtgttccag cggccgccga
     1261 ttcctgacga aacggcatct cgatcagcga caagttcgtg gctggccttc cgcggtcctc
     1321 gatggcagcg caccattctt aggaatgctc ctcccatcgt gtcgcgaggc ggggacgcta
     1381 tacgtgccaa agatgcggaa ctcagtgatc ggtggctagg aatatgtccg ggtgttccga
     1441 tgagggtgtt gggggatgcg taaacccgac ctgatattga ataaagtggg catagatcat
     1501 cgaagagagc ccgcaggcga tctcggtgcc gtgaaatctt gctctgaagt gatcctagca
     1561 gctcgcccat ccccagcagc gacatgatga ttcgtccggt cacagggacc accgaagcga
     1621 ccagcgctac ggctagtgta aacgctagaa ctccgaggct aatgcctgcc aacacacagg
     1681 gaaaaagttt catcataaaa ttcctaagca gtgcataacc ggctgtcaag caccggaacc
     1741 gtcgaccgaa ggtgaaccga agccgtgtat tcaccgtcgt tcagcgtcta ctaggcaaca
     1801 tctcaaaaga ttcagccggc acatttctgc tacgattcca gcgaatcaca ccctccggcg
     1861 acataaaaaa aggtcccctt gtgggggacc tttgtagccg ccgggagagg agggttaaaa
     1921 gccggtgcta aattacgcgg ctttggattc gagcttctta ttctcttttt gctcgatctg
     1981 aaccgggatc ttgcgtccag caagctgcgc cggaagcggc acatggatct caagcacccc
     2041 gtgttcatag cgggcggcga tcttgtcagc ttcgatgcct tgcggcagag ttaccgtccg
     2101 ttcgaatttg ccgtaagaag tctcctgata accctctctc ttctcttctg attttcgttc
     2161 cccggaaatc gacagaacgt ttccttccgc ctggacctgc acgtcattgg ggtttactcc
     2221 cggaagatcg aatcgcaaga cgtactcgtt gtccttgcgg taggtctcca tccgaggcac
     2281 ccagttgccc aaagaagtag tctcagggtg tccgaagaac cgaccaaaca agtcgtcaat
     2341 atcacggtgc cagttagaca gttcttccga aggattaaat ctcatcagtt caccgaacat
     2401 acctaatacc tcctttcctc atcgagaatt tctaactgaa atttaatccc gatgagagac
     2461 ctgtcaaccc cacttcggat aaccgaagcc tgcttttgcc cgggtaatct aaattcaaat
     2521 gctccagtta cgcagaaaca catccgtgct tcgtaccgac catggcacgc gccggattcg
     2581 gccggagcgg tagcgctgcg gtatgttcat gataggttat ttgcaccgtc ggaagcgctt
     2641 gcttgattgt gcgttcgtcc aagcactgtt ccccatcggc aactgggatg gttatatata
     2701 taaggacgtg tggtggagga ttgctcttct tggtggcgct gtctggggga ccccatgaag
     2761 agcgaccgaa tggacggctg gaaaccagcc gaatagaaca tcgaattcag ctgcaacact
     2821 gcctgcagta cactggtgta atacaaatga gcgtggccta tgcctgtgag cgttcgtcta
     2881 gatgccaaga caaaatcgtt gatcggccgg ttggctcgga aacgacgaca gacaaagtcc
     2941 gaagttatac gcgacgccat cgatgttctt gccaagcagg aagagaaaag cacggaaaag
     3001 aagcgtcctt atgatctggt ggctcacttg atcggctgtg tggacagcgg cggcgcgaat
     3061 ctatcgcaga ataccggtga aaagtttgcg aagctcttgc gggagagggc tcgtgcccgc
     3121 cgttctcgtt ggacacgggg gccactggtg gccttgctgg accgaagtga ttcccatcac
     3181 ctcagctgtc aagagacttt cagttcgctg gacgattcgc tggtcaccgt atggccggtc
     3241 gtgacggagg tcctatacct atatcctata cctatatatg ttgagagcct actggcaggc
     3301 gccagatgcg ctttgggaaa tgatccagtc cggggccgtt cagatacttt cgctaggcat
     3361 cgatgatatc ccgcgcatga gagagctgat gcgcaaatac cgagatctgc cgatggacct
     3421 tgcggatgcc gggttggtgc gggtcgccga gcgcgaacgg ctgcgccgca ccttcacatt
     3481 ggaccgccgc gactttcaag tttatcgccg tcacgaatcg gtcgcttcac catccttcca
     3541 cctcgataga aactttcata cctttaatat taaacaaaat tcggttgttt cgcaggatgc
     3601 tggcagagcg tacgaagatc gagactcgct gtcgatttca caggcgg
//
LOCUS       DASUTG010000408         5129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_64012, whole genome shotgun sequence.
ACCESSION   DASUTG010000408 DASUTG010000000
VERSION     DASUTG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64012"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..922
                     /locus_tag="VF089_09325"
     CDS             53..922
                     /locus_tag="VF089_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX6174204.1"
                     /translation="MTETPRKALIANIADALLPPEAALACETAGAAKAGRDPLALIIL
                     GLLAGAFIALGANFMTVVLTGTAELPWGVARLLAGLVFSLGLILVIAGGAELFTGDSL
                     MIVACASRRITLGALLRAWSLVYVGNIAGAVATAGLMYLAGQHGFAGGAVGKTALDVA
                     SAKAALPTTQLFFLAVLCNVLVCLAVWMSFGARSTTDKILVIVPPITAFVAAGFEHSI
                     ANLYFLPYGLMIKAWATPEYWAAIGQDATAYSELNARSALHNIVVATIGNLVGGSLMV
                     GAVYWFVYLRRRT"
     gene            949..2556
                     /locus_tag="VF089_09330"
     CDS             949..2556
                     /locus_tag="VF089_09330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6174205.1"
                     /translation="MTVTLTFHGAAGTVTGSHYLLETPTARVAVDCGMFQGPKTLKEL
                     NYKPLPSDPKHIDAVVQTHAHTDHSGMLPKLVKSGFRGPIFATQGTRDLLEWMLADSA
                     SIQEAEVERLNRENRRRGRPEVQPVYTIEDALAALHRIRMVEYESWQPIAKGIRGRWW
                     NAGHILGSASIELDVDTGNDDLPRLSILFSGDLGPQDGALQQPPRGPQGIDYVLCEST
                     YGNRDRPQLHDEQRRSALAREVTQALGNGGNLIVPAFAIERSQELIGDLTYLMVRGQL
                     PKAPIYLDSPLATRATEVFERHLHLLDDVDPKAGSPFRAPNVHYVQTVEQSMALNRIS
                     GGAIIIASSGMCDAGRIRHHLRHHLWHPRTTVLLIGYQAPGTLGRLLQDGATAVRIMG
                     EEIQVKARIKKIEIYSGHADRHDLLAWVKARLPVHRGLFLIHGETAALAGMRENALSL
                     GIAPDKVFTPDLDQCYRLDRVAGALAIGGTPRLEPVRSEEAKQGWDWHNELSAFSLEL
                     RRILDHKHSDEARMTVLKDMRRVLEDR"
     gene            3598..>5129
                     /locus_tag="VF089_09335"
     CDS             3598..>5129
                     /locus_tag="VF089_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6174206.1"
                     /translation="MTIRNLDSLFKPRSIAVIGASPNLGTVGSVVVKNLHTGGFSGTI
                     YLVNPKYASFEGQPVYADVAALPGVPDLAVIATPPASVPGLISQLGRRGTKGAVVITA
                     GFTSDSGSALKQAMLDAAQPHLLRIVGPNCLGLLVPPLGLNASFAHLQPHRGGLAFIA
                     QSGAILTSVIDWARPRGIGFSRMVSLGDMADVDFGDMLDYLSTDPETSAILLYIEMVT
                     HARKFMSAARIAARTKPVVVIKAGRHAESARAVASHTGAMAGSDLVYDAAFRRAGLLR
                     VLDLEELFSAVETLAMSATPPGDRIMILTNGGGAGILATDALVDAGGQLAELSANTRK
                     QLDEVLPPTWSHANPIDIIGDAPPARYAAAMTALLADPGSDAILVLSCPTAISAGADA
                     ARAVIDTLKNGRRPVFTCWLGGETASTARRLFADARLPSYDTPEQAVRAVMHMVNYRR
                     NQEQLMQTPPSISRELGCDIARARTVIEHVLAEKRDLLTLPESMTVLAAYGVPVNDVM
                     VAT"
BASE COUNT         1019 a         1565 c         1480 g         1065 t
ORIGIN      
        1 tgacgttgat cctttggagc gaatcttccc tcgcatcgct gtgtgcacat aaatgactga
       61 aacgcccagg aaggcgctta tcgccaatat cgccgacgca ttgctaccgc ctgaggcggc
      121 gctcgcctgc gagaccgccg gtgccgccaa ggcgggacgc gatccactgg cgctcatcat
      181 cttgggattg ctcgcgggcg cattcattgc ccttggcgcg aacttcatga ctgtcgtact
      241 gaccggaacc gccgagctcc cttggggcgt tgcgcgcctc ctcgctggtc ttgtgttttc
      301 gctcggactt atcttggtga ttgcgggtgg cgccgagcta ttcaccggcg actcgctgat
      361 gatcgtagcc tgtgccagcc ggcgcatcac gctcggggct ctattgcgcg cctggtcgtt
      421 ggtctatgtc ggcaatattg ccggcgcggt ggccacagcc gggttgatgt acctcgcagg
      481 gcagcacgga tttgccggcg gagctgtcgg caagacggca ctcgacgtag cttctgcaaa
      541 agcggcacta ccgacaaccc agctgttttt cctggccgtg ctctgcaatg tattggtctg
      601 tctcgccgtg tggatgtcgt tcggggcgcg tagcacgacc gataagattt tggtaattgt
      661 tccgcccatc acggccttcg tcgccgccgg cttcgaacac tcgatcgcca atctctattt
      721 tctaccctat ggcctaatga tcaaggcctg ggctacgccg gagtactggg ccgccatcgg
      781 gcaggatgcg acagcctatt cggagctgaa cgcgaggagc gcgctccata acattgtcgt
      841 cgcgaccata ggcaatcttg tcggtggtag cctgatggtt ggtgctgtct actggttcgt
      901 gtatctgcgc cgacggacat aaccaccggt ctttcaagag gatgcacaat gaccgttacc
      961 ttgaccttcc acggtgctgc cggaactgtc acgggctcgc actatctcct cgagacgccg
     1021 acggcgcgcg ttgccgtcga ttgcggcatg tttcaggggc cgaagaccct caaggaactc
     1081 aattacaagc cgttgcccag cgatcccaag cacattgacg cagtcgtaca aactcacgcc
     1141 cataccgatc attccggcat gttgccgaag ctcgtgaaat ccggctttag aggcccgatc
     1201 ttcgcgaccc agggaacgcg cgacttgttg gaatggatgt tggcggattc cgcctcgatc
     1261 caggaggcag aggtcgagcg gctgaaccgc gagaaccgcc ggcgtggccg gccagaagtg
     1321 caacctgtgt acaccatcga ggatgcgctc gcggccctgc accgaatccg tatggtggaa
     1381 tatgagtcat ggcagccgat cgccaaaggc attcgcggac gctggtggaa tgccggacac
     1441 attctcggct ccgcctcgat cgagctcgat gtggacaccg gcaacgatga cctgccacgt
     1501 ctcagtattt tgttctccgg cgatctcggg ccgcaagacg gtgccttgca gcaaccgccg
     1561 cgcggaccac aaggcataga ttacgtactg tgcgaatcga cctatggcaa tcgcgaccgg
     1621 ccgcaactgc atgacgaaca acgccgctcg gcgctcgccc gtgaagtcac gcaggctctg
     1681 ggcaacggcg gcaatctgat cgtacccgct ttcgccatcg agcgcagtca ggaactgatc
     1741 ggcgatctta cctacctgat ggtgcgtggc cagttgccca aagcaccaat ctatctcgat
     1801 tcaccgctcg ccacgcgtgc gactgaggtg ttcgagcgcc accttcatct actggacgat
     1861 gtcgatccga aggccggcag cccattccgt gcgccgaatg tgcactacgt ccaaaccgtg
     1921 gaacagagca tggcactgaa ccggatcagc ggcggagcca tcatcatcgc ctccagcggc
     1981 atgtgcgatg ccgggcgaat ccggcatcat ctgcgacatc atctctggca tccgcgtacg
     2041 acagtcctgc tgatcggcta ccaagccccg gggacgctcg gacgcctgct gcaggatggc
     2101 gcgacggcgg tcaggatcat gggcgaagag atccaggtca aagcaagaat taagaagatc
     2161 gagatctatt cgggccatgc cgatcgccac gatctactcg cctgggtaaa agcccgcctg
     2221 ccggtccacc gcggattgtt cctgattcac ggcgagacgg ccgcactggc tggcatgcgc
     2281 gaaaatgcgc tcagtctcgg catcgcccca gacaaagttt tcacgcccga tctcgatcag
     2341 tgttatcgac ttgaccgtgt tgcgggcgcg cttgctattg gcggaacgcc gcgcctggag
     2401 ccagtacgca gtgaggaggc caagcaaggc tgggactggc acaacgagct ttccgccttt
     2461 tcactggagc tacgccgcat cctcgaccac aagcatagcg acgaggcgcg catgacagtg
     2521 cttaaggata tgcggcgagt tctggaagat cgctgaagta tcataactaa tgctccggga
     2581 tgtggcgttg gccgcgcatg aagcctcagt tttctgggag cacctgacga atcaacggcg
     2641 cacaacacat taatctgatg gtcgttgcta tggtctttgc cttctgcctt caaagcctgg
     2701 cgatgcgtct cgaggaaact gcggtaggct tgagcatcac cataatcctc gaactttaca
     2761 tggcgtttgt gtgcaccgat gacacgacgg gcatgttcaa ttttgggcaa ctaatccatt
     2821 cgcatagtaa gcgcaattca gcttctcaat cagattgaga tgcggcagat gcgctctgtc
     2881 aaaaacgaag aaagtcgcta cgcggaacct tagctatccc ataaactcta aaacagatgc
     2941 tttggtagac agtaacaaac aagcctagca agagtacgag taaaacaacc gcgtaaatta
     3001 tgggagccgt gataatcacc agcggccgcg caccgaggat atacttcata agtcttatct
     3061 gccgttcgcg atggcgcgcc gaagcagctc ttgctcaaag cggacctttc catgctcaag
     3121 cccaatgcgc ggcatcgccc gctgcttcga gtcctccagt gtttcaatct tacgaatcag
     3181 tttcgctttt ttgaggactc attggtcgcc cctgtcgcca ctaacgcccg cacattccat
     3241 cggcttgcac attgatggat gcgagttgtc tcagccatct actattccga ctaggcgcac
     3301 cacgctgggg gccgattggg gtggacctag gcatgagata ccacgcggtc gtggtgcgac
     3361 cggctgggaa agctcgagcc tcctagcccc gcaggcggac aaccgtcgtc ctgcgatcca
     3421 aacgaagatc gacagcacgg cgtcatcttc cggcccgaac caagctagag agcgatcatt
     3481 gctcttgcca aagggcgagg cgaatggtcg acatagagcg ccatatccga tgatgtcaga
     3541 aatggcccag attgctggac ccagccccaa tcgtcctttc gatggagcgc cgacggtatg
     3601 acgatccgca acctcgacag cctgtttaag cccaggtcca tcgcagttat tggcgccagc
     3661 ccaaatctgg ggacggttgg ctcagtcgtc gtgaagaatc ttcacaccgg cggcttctcc
     3721 ggcacgatct atctcgtgaa ccccaagtat gcgtccttcg agggccagcc tgtctatgcg
     3781 gacgtcgccg ccctgccggg ggtccccgat ctggcagtga tcgctacgcc cccggcaagt
     3841 gtgcctggct tgatttcgca gctaggcaga cgcggcacca agggcgcggt cgtcattacg
     3901 gctggattta ccagcgatag cggttccgcg ctgaaacagg cgatgctcga tgccgcgcaa
     3961 ccccaccttc tccgaatagt tgggcccaac tgcctcggac ttcttgtgcc gccgctcggg
     4021 ctgaacgcca gtttcgccca tcttcaacca catcgcggcg ggcttgcctt catcgcgcaa
     4081 tccggcgcca tcctcacctc cgtcattgat tgggcgcggc cacgcggcat aggtttctcg
     4141 cgcatggtgt cgctaggcga catggccgat gtcgatttcg gtgacatgct ggactatctc
     4201 tcgaccgatc ccgagacaag cgcaatcctt ctctatatcg agatggttac ccatgcccgc
     4261 aagttcatgt cggcggcgcg tatcgccgcg aggacgaaac cagtcgttgt gatcaaggca
     4321 ggacgccatg ccgagtcggc gcgcgctgtc gcctcacata cgggagcgat ggctgggtcg
     4381 gatctggtct acgacgccgc cttccggcgc gccggcttgc tccgtgtgct ggaccttgaa
     4441 gagttgttct ccgcagtcga aacgctggct atgagtgcta cgccgccagg cgatcggatc
     4501 atgatcctga ccaatggcgg cggtgctggc attcttgcta ctgacgccct ggtagatgct
     4561 ggcggccagc tcgcggagtt gtccgcgaac actcgcaagc aacttgatga ggtcctgccg
     4621 ccgacgtggt cacacgccaa tcccatcgac attatcggtg acgcgccgcc agcccggtac
     4681 gcggcagcga tgacagcgct gctggccgat ccggggagtg atgccattct tgtattgagt
     4741 tgtccgaccg cgatttcggc cggcgccgac gctgcccgcg ctgtcattga tacgctcaag
     4801 aatggccgca ggccggtctt cacctgttgg ctcggcggcg aaaccgcgag cacagcacgt
     4861 cgtttgttcg ccgatgctag actgccgagc tatgacacgc cggagcaggc ggtgcgcgct
     4921 gtcatgcaca tggtgaacta ccgccgaaac caagagcagc tgatgcagac cccgccgtcc
     4981 atcagccgag agctcggctg cgatattgct agagctcgca ccgtgatcga gcatgtcctc
     5041 gccgaaaagc gagaccttct gacactaccc gagtcaatga ctgttcttgc cgcctatggt
     5101 gtgccggtca atgacgtgat ggtagcaac
//
LOCUS       DASUTG010000409         4588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_657214, whole genome shotgun sequence.
ACCESSION   DASUTG010000409 DASUTG010000000
VERSION     DASUTG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657214"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VF089_09340"
     CDS             <1..682
                     /locus_tag="VF089_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174207.1"
                     /translation="LAACMGYPASQHPMAAKELEVMGIGSQERPARLEEMIQALRVLW
                     SDEHASFQGKFYSFQDVNLLPKPAQRPCPIYIAGTPRSNQIGDRGVERSLRRIARYAD
                     GWMTNQIELERFKDYHGRLRHMLLEEGRDPNAFKTVLYYGISVNADRDQAFREAKTFL
                     DTYYQKDFTRQGVEIWNACGPVEHCVESVEEFIEAGVDHVTIRPIGNDLAEQLGIYLN
                     EVLPVLRS"
     gene            complement(731..2290)
                     /locus_tag="VF089_09345"
     CDS             complement(731..2290)
                     /locus_tag="VF089_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6174208.1"
                     /translation="MFETLSNFFTSEGFEPHGHCFLWQRPLLWLYVASDSLIAISYYI
                     IPIALVIFVRKRQDLAFNWMFLMFSAFIFACGTTHLMGIWKLWEPVYWLDGSVKAITA
                     GLSVATAATLWPLIPQALALPSPRSLELVNQELQQQIAEREFAAQKLRETETLFRSLL
                     ESAPDAMVIVDAQGMITLVNSQTERLFGYERKELLGKPVEHLVPARFRERHQDHRGLF
                     AKNPHVRPMGTGMELFAVRRDGSEFPVEISLSPIATGRELLMSAAIRDITERKRAERL
                     LQEKERLATLGTTAAVFAHEIGNPLNGISTSLQIVKSILETDKIDPVLHETLEIAFQE
                     VQRLATLLSDYRTFARPQRLNLRPADVTQIIEEVLSAQLPMYRGTKIALQTELENLPA
                     MVVDPEKIKQVILNLCKNAVEAMPNGGSLTVKATGSEGGVILEVTDTGEGIAHGVDVF
                     QLFRTTKPDGTGLGLPIVQQIISDHRGTITYRSEIGKGTTFTIGLPAGVLIAAGANAE
                     PFDDAALKKNL"
     gene            complement(2357..2713)
                     /locus_tag="VF089_09350"
     CDS             complement(2357..2713)
                     /locus_tag="VF089_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174209.1"
                     /translation="MSCNLLLVEDNPLSRRNLAMFLQQAEFTVHQTDNGEAALELISR
                     VDFDIVISDFRLPGKVNGLDVLKYQSFRGSGKRLILITAFGSEQAQAEAAAVGALYFE
                     KPISLRDLLDTIQFTH"
     gene            complement(3024..3350)
                     /locus_tag="VF089_09355"
     CDS             complement(3024..3350)
                     /locus_tag="VF089_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6174210.1"
                     /translation="MPKEPLARARIRIWIDYCNTRVQRAAGYIAHNYKVEESGTELRT
                     YLENLDRYMRGREYLTDEYSLADITYIPFFVRRERYQVNIGDDLPHLKSWMERLLARP
                     VVRATP"
     gene            complement(3372..3545)
                     /locus_tag="VF089_09360"
                     /pseudo
     CDS             complement(3372..3545)
                     /locus_tag="VF089_09360"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            3871..>4588
                     /locus_tag="VF089_09365"
     CDS             3871..>4588
                     /locus_tag="VF089_09365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174211.1"
                     /translation="MIIDTHFHAFPKKFLELVPEQSQSDVRGIGFHGFDHQEYLNVMD
                     QHGIDTGVLSNTGGRIEQGGDRRKALELCTIINDAFAEAHAKHPQRFKAFARLPMVNL
                     DDCVRELERCYGDRNMHGVILSTNLAGRYLDEPEFKPFWDALEASGKPLFLHPANAPC
                     QANWNKYSLHQKLLWPTDSSLAISRIVYSGIFDRYPSIKLIASHLGGMILLYLDRLNW
                     REGNVECRQEPEEYFKEIYYD"
BASE COUNT         1073 a         1244 c         1209 g         1062 t
ORIGIN      
        1 gctagccgcg tgcatgggat acccggccag tcagcatccg atggcggcca aggaacttga
       61 ggtgatgggc atcggcagcc aggagcggcc ggcgcgattg gaagaaatga tccaagctct
      121 gcgagttctc tggtccgacg aacatgccag cttccagggc aagttctact cgttccaaga
      181 tgtgaatctc ctgcccaaac cggcgcaacg gccttgcccg atctacatcg ccggcactcc
      241 gcgatccaac cagatcggcg atcgtggagt ggagcgttcg ttacggagaa tcgcccgtta
      301 cgccgacggc tggatgacca atcagattga gcttgaaagg tttaaagatt accacgggcg
      361 attgcgtcac atgttgttgg aagaggggcg ggacccgaat gccttcaaga ccgttctgta
      421 ttacggcatc tcggtcaatg ccgatcgcga tcaggcgttt cgcgaagcaa aaacttttct
      481 cgatacgtat tatcaaaaag acttcacgcg ccagggtgtt gaaatctgga acgcctgcgg
      541 gcctgtggaa cactgcgttg aatctgtaga ggaatttatc gaggcaggag tcgaccacgt
      601 caccattcgg ccgatcggca acgatttagc tgagcagctt ggcatctatt tgaacgaggt
      661 tcttcctgtt ttacgctcat aagcttgccg taaatcgcag cccaacttca ccgggaaaga
      721 atagttggag ctacaaattc ttctttagtg cagcatcatc gaaaggctcc gcattggcac
      781 cagccgcaat tagaaccccg gcaggcaacc caatggtgaa cgttgtgcct ttgccgattt
      841 cacttcggta tgtgatcgtt cccctatgat cgctaatgat ctgctgcaca atcggaaggc
      901 ccaggcccgt gccgtcgggt ttggtagttc tgaacaactg gaagacatcg acgccatgcg
      961 ctattccttc gcccgtatcg gtaacttcca gaataacccc tccttctgaa ccagtggcct
     1021 tgaccgtcaa gcttccgccg ttcggcatgg cttcaaccgc gttcttgcaa agattcaaaa
     1081 tcacctgctt tattttttca ggatcaacga ccatggcggg caggttttcc aattcagttt
     1141 ggagggcgat tttcgtcccc cggtacatcg gcaactgagc gctaagcacc tcttcaataa
     1201 tctgcgtcac atcagctggg cgcaggttaa gcctctgagg tcgcgcaaat gtgcgatagt
     1261 cggaaagcaa cgttgccagc cgctggactt cttgaaatgc gatttcgagc gtttcgtgta
     1321 agaccggatc gatcttgtca gtttctaaga tcgatttcac gatttgtaac gacgtggaaa
     1381 tgccattgag cggattaccg atctcatggg caaataccgc ggcagtggtg cccaatgtgg
     1441 ctaatcgctc tttttcctga agtaatctct ctgcccgctt gcgttccgtg atgtcgcgaa
     1501 tcgccgcgct catcagaagt tctcgccccg ttgcaatcgg gcttaagctg atctcgacgg
     1561 ggaactcgct gccgtcgcgc cgaacggcaa atagctccat gcccgttccc atcggccgaa
     1621 cgtgcggatt tttcgcaaac aagccgcggt gatcctgatg tcgctcgcga aaccgcgcgg
     1681 gaacaaggtg ttcgaccggc tttccgagaa gttcttttcg ctcataaccg aacagtcttt
     1741 cggtttgtga atttaccaag gtgatcattc cttgtgcatc gacgatgacc atggcgtcag
     1801 gcgccgactc gagcaatgag cggaaaagcg tctccgtttc tctcaatttc tgcgcggcga
     1861 attcgcgctc ggcaatttgt tgttgcagtt cttggttgac cagttcgagc gagcgcgggc
     1921 tcggaagcgc cagcgcctgc ggaatcagag gccatagggt cgcggcagtt gccaccgaaa
     1981 gaccggcggt tatcgcctta acacttccat ccaaccagta gaccggttcc cagagcttcc
     2041 atattcccat caaatgtgtg gtgccgcacg caaagatgaa tgcgctgaac atcaggaaca
     2101 tccagttaaa cgcaagatct tgtcgcttac gaacgaagat aaccagagcg ataggtatga
     2161 tgtagtaaga aatggctatc agcgaatccg acgctacata caaccagagg agcggtctct
     2221 gccagagaaa acagtggccg tgcggttcga agccctcgga cgtaaagaag ttgctcaacg
     2281 tttcgaacat agtaattccc tcgcaggcag ccaaccggcg cgaagtgact cggctatgaa
     2341 acgggcggtg gcgttgttag tgcgtaaact gaattgtatc aagcagatcg cgcaaagaga
     2401 tcggcttttc gaagtaaagc gcgcctaccg ctgcggcctc tgcttgagct tgctcggaac
     2461 cgaacgcagt aatgagaatg agacgttttc ccgaacccct aaaactttga tacttaagga
     2521 catcgaggcc attcaccttt cccggcagac gaaagtcaga aatgacgatg tcaaaatcga
     2581 cgcgggaaat aagctccaac gcagcctcgc cattgtcggt ctgatgaacc gtgaattcgg
     2641 cttgctgaag aaacatcgcc aagttccggc gggacaaagg gttgtcttca accaggagca
     2701 gattacacga catgccgggc caaattaaac caagattgca ttataagcaa caaaaacctc
     2761 accttgcgcg ggcaaattgg gctttcaaaa cagcttctgt gaagctccag tcgctgccaa
     2821 gcagcgccgc ccgcgctagc tattacaacg tattttggtg gattaagccg ctgcagaaaa
     2881 cagcagcttt gcagttttgc tacttcgatg gacgcactat caagccatca agggcgcgtt
     2941 tgtgcgcttc ttgtcactta gtaacaaaac tccgcttgac gtgagctctg atgtttgtcg
     3001 ctcagagcct atccggcagc cggttaaggc gtcgcgcgca ccacgggccg cgcaagcaaa
     3061 cgctccatcc aagatttgag gtgcggcaag tcatcaccaa tgtttacctg ataacgctcg
     3121 cgtctgacaa agaaaggaat gtaggtgatg tccgccagag agtattcatc ggttaggtac
     3181 tctcggcctc gcatgtagcg gtcgagattt tcgaggtacg ttcgaagctc ggtgccagat
     3241 tcctcgactt tgtagttgtg cgcgatataa ccggcggcgc gctgcacgcg ggtgttgcag
     3301 tagtcgatcc aaattcttat gcgcgcccgg gccagcggct ctttcggcat caagggcacc
     3361 tcgggatacc gttcttccag gtactcatcg ataatcgcgg actcgtaaac aaccgcatca
     3421 tcatcgacca gcaccggaac cttgccataa ggatttagct cgagaagatc ttttggcttg
     3481 ttgcctagat cgcattcgat caaatcatag gggatctttt tttccgccag gacaagcctt
     3541 gtcctgtgag cgtagggtca ggggccgtaa gaaaagagtt tcatgaggtc acctccgtct
     3601 gatcgcgttg taccacctga tagccgcggc ggcaacaatt ctgattgatc cgaaatcgtt
     3661 tgcagtcaac acacagatcc atcgcttctg gctttttttc tgatgcgcca tgttcgcgct
     3721 catatgcagg cgcagatgtg ttgacacccg cctacgctat gcgataagcg gccctatagc
     3781 cacttcactg gaatgattgt ttcgccctgc gtcacgccgg ggttggttat ctatttaggt
     3841 atgacgtgag cgttagctag agggggccgc atgatcatcg acacgcactt tcatgccttt
     3901 cccaagaaat ttctcgagct cgtgcccgaa caatcacaga gcgatgttcg cggaatcgga
     3961 ttccacggct ttgaccatca ggaatatctc aatgtgatgg atcaacacgg catcgatacc
     4021 ggcgtgctct ctaacaccgg tgggcggatc gaacagggcg gcgaccggcg caaggcgctg
     4081 gagctgtgca cgatcattaa cgacgcattt gccgaagctc acgccaagca cccgcaacga
     4141 ttcaaagcgt tcgcacggct gccgatggtc aaccttgatg attgcgtccg cgagctcgaa
     4201 cgttgttatg gtgatcgcaa catgcatggc gtgatcctgt cgaccaatct cgccggacga
     4261 tacctcgatg agccggaatt caagcctttc tgggacgcgc tcgaggcaag tgggaaaccg
     4321 ctatttcttc acccagccaa tgcgccatgc caagccaatt ggaacaagta ctcgctgcac
     4381 caaaaactcc tctggcccac cgacagctcc ctggcgatat cgcggatcgt ttatagcggg
     4441 atattcgatc gctatccgtc tatcaaactc attgcctcgc atctgggcgg catgattctc
     4501 ctttacctcg accggctcaa ctggcgcgag gggaatgtag agtgccggca ggagccggag
     4561 gaatacttca aggaaatcta ctatgaca
//
LOCUS       DASUTG010000410         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_954145, whole genome shotgun sequence.
ACCESSION   DASUTG010000410 DASUTG010000000
VERSION     DASUTG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954145"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1510)
                     /locus_tag="VF089_09370"
     CDS             complement(191..1510)
                     /locus_tag="VF089_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174212.1"
                     /translation="METTIEQARPASQQKFFYGWYIVGVGFLANVASAFSLASTMSVF
                     LKPLTADLGVSRGVFSLLRSGEGLIAAFLAPVIGTLMDRYGGRWLMVFGAIIASLGYV
                     VLSHVDNFIQFSIVRLSLITVGDSFMGYIVINLAIAHWFSRKRGRALALTSMGIGFAK
                     VGMPVLAASLIVWLGWRQTWLVFGIVTLVLVVGPALLIVRRSPEDMGLHPDGALSAAP
                     KDEPATAIAPTSSRTQQRIRDDVEWTRNEALHTRAFWLLVVTFGISSIGVTGLNLHVF
                     PYVNDLGHPPVVAATVMSVIASMQLASPILWGILAERIDTRFAATLRFVVQGIGLGLA
                     VITDNLLCLYAGFLLYGIGLGGNMVLPDILWAEYFGRRSLGKVRGLGLLISQAVAALG
                     PPFFGFLFDLTGAYDLSFILFGAALITSAFLSLMLRPPVKRRAERSN"
     gene            1739..>2241
                     /locus_tag="VF089_09375"
     CDS             1739..>2241
                     /locus_tag="VF089_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6174213.1"
                     /translation="MKLSIIVMSLLLPASSLYAQENVSVRPPSVTTQGEAVISAEPDQ
                     AQIDIGVLTQARTAPDAAKENAEKLSRVLAQIKKLLGKGDEVKTTGYSLNPNYRYPQG
                     GKPEITGYTAANVLQIRTGNLTGVGKLIDASMQAGANQINRLVFTLKDEHSAQVQALR
                     MAAQEAQS"
BASE COUNT          601 a          644 c          613 g          383 t
ORIGIN      
        1 cgcgctccag taaagagata tactgccgca caggcagatc atgcacgcgc gcccgcacta
       61 aagcgccttt accgcgcagc aatacgtgtt gctctttcaa gcgagctatc tgctgatctg
      121 cggttaaggt ggcgaaatca agatgaaccg tttgcaaagc tattggcatg ctccctcact
      181 cagcacttcg tcagtttgag cgctcggccc ttctcttcac cggcggccgc aacatcaggc
      241 tgaggaacgc cgatgtaatc agtgctgcgc cgaaaagtat aaaagaaagg tcgtaggctc
      301 cggttaaatc gaacaaaaac ccgaagaatg gcggtcccaa agcggcaacg gcttgggaaa
      361 tgagcagacc caggccgcga actttgccca gtgagcggcg gccgaagtat tctgcccaga
      421 gaatatccgg aagcaccatg ttgcctccca agccgatgcc gtaaagaaga aatccggcat
      481 agagacagag gagattatcg gtaataaccg ccagtccgag accgatcccc tggacgacaa
      541 accgcaaagt tgcagcaaag cgggtatcga ttcgttcggc gagaataccc cacagtatcg
      601 gcgaggcaag ctgcatggat gcaatcacgc tcatcaccgt ggcggcgaca accggcggat
      661 gacccaaatc attgacgtac ggaaacacgt gaagattcaa gcccgtcact ccgatgctgg
      721 aaatgccgaa ggtgactacg agcagccaga acgcacgagt gtggagcgct tcgtttcgtg
      781 tccattcgac atcgtcgcga atcctctgct gcgttctcga agacgtcggg gcgatagcgg
      841 tggctggctc atccttcggc gcagctgaca aagcgccatc cggatggagt cccatgtctt
      901 cgggcgagcg gcgcacgatc aacagggccg gcccgactac caaaaccaac gtgacgatac
      961 cgaacaccag ccatgtttgg cgccatccaa gccagacgat caacgatgcc gccagcacag
     1021 gcatgcccac cttggcaaaa ccgatgccca tgctggtaag cgccagcgcc cggccgcgct
     1081 tgcgcgaaaa ccaatgggca atggccagat tgattacgat atagcccatg aacgaatcgc
     1141 caaccgtgat cagactcaag cgcacgatgc taaactggat aaaattatcc acatggctga
     1201 gtacgacata gccgaggctg gcaatgatcg caccgaaaac catcaaccag cggccgccat
     1261 aacgatccat caacgtgccg atcaccggcg ccagaaatgc cgcgatcagc ccctcgcccg
     1321 agcggagcaa agagaaaacg ccgcgcgaga cgcccagatc ggcagtcagc ggtttcagaa
     1381 acacgctcat ggtgctggcg agagaaaaag ccgaggcaac attggcgaga aaaccgacgc
     1441 cgacgatgta ccagccgtag aaaaacttct gttgggatgc cggacgcgcc tgttcgatgg
     1501 tcgtttccac atctagcctt taacaggaat caaactggac atccaattta atacgtgatg
     1561 gcaagctagc gcggtgtaaa atgcgcggta ggttgctcaa cggaaaagag atcagcagat
     1621 cacgacggca acggcatcgg ctataaggtt cgcaggactg tggcagcaga gcgggtgacg
     1681 actggcggtg ctattgttta tgcgatagca ccgtctaata tctagggacc catctcacat
     1741 gaaactatcg ataattgtaa tgagtctcct tctgccggca tcgagcctct acgcccaaga
     1801 gaatgtcagc gtcagaccgc cttccgtcac tacgcaaggg gaggcggtca tcagcgcgga
     1861 gccggaccag gcgcaaatcg acatcggggt tctcacgcaa gcgcgcactg ccccggatgc
     1921 agccaaagaa aacgccgaga agcttagccg tgtgctggcc cagatcaaga agctcctcgg
     1981 caaaggcgac gaggtcaaaa ccaccggcta ctccttgaat cccaactacc gctatcccca
     2041 aggcggcaag ccggaaataa ccggttacac agcagccaat gttttgcaga tcagaacggg
     2101 aaacttgaca ggagtcggca agctaatcga cgccagcatg caagccgggg ctaatcagat
     2161 caaccggctt gttttcacat tgaaagatga gcattcagcg caagttcagg cgctccgcat
     2221 ggcggcgcaa gaggcgcagt c
//
LOCUS       DASUTG010000411         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_805015, whole genome shotgun sequence.
ACCESSION   DASUTG010000411 DASUTG010000000
VERSION     DASUTG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805015"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1767
                     /locus_tag="VF089_09380"
     CDS             334..1767
                     /locus_tag="VF089_09380"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6174214.1"
                     /translation="MTDVTDMRGYVELLEEMGELRRLEGVDLNLEVGALTERAAEKEG
                     PALLFSNFKEYPAGFRVISNVFRTCRRTGPAMGIDPELKGVDYLNAWRKKLAAYKPVP
                     VQQVEGGPVFENQMVDDQVDLYKFPVPKWHDLDGGPYIGTGCGVITKDPETGKINIGT
                     YRVMIQNKNTLSVKMNMGKHGRLAFERARDAGKPLPIAITFCQGPAIFLAAQMPLPPD
                     VNEFEFAGYLQGAPVPVARGAATGLPIPANAEIVVEGELPPMKDQDMPKEGPFGEWPG
                     YFTEENVGETPIMNVKRIYYRNDPIILGAPPLKPPASYLPIPLGAATLWEQLDKAGIP
                     EVKGVWGFVYGGQPGPFTIISIKQRYAGHSKQALLVAAGARAGAYGGKFVVVVDDDID
                     IANPADVIWAIATRCHVREGLDVVKGVWASVCEPALPPEERSPRGYTSDRVLIDACRP
                     FKWMDQFPPVNAFTSDFKDQIESKFKI"
     gene            1842..2477
                     /locus_tag="VF089_09385"
     CDS             1842..2477
                     /locus_tag="VF089_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6174215.1"
                     /translation="MGSIEALSLAIGTAWTSGINLYATVAALGLAGHFGMIQLPQSLE
                     VLTNPLVIAVACIMYAIEFFADKIPYVDNGWDVLHTFIRVPAGAILAARSLGDMSPAL
                     ETAALLAGGSIALAAHGTKATSRLAINSSPEPFSNWIASFVEDIAVLGGIWIIFNHPW
                     LMLALVVVFLAVMIWIAPKIFRLSKRGFTMLCKRLGGSKQEQATTSTPQSP"
     gene            2534..3391
                     /gene="hemQ"
                     /locus_tag="VF089_09390"
     CDS             2534..3391
                     /gene="hemQ"
                     /locus_tag="VF089_09390"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX6174216.1"
                     /translation="MEPHAEKRAGDLPPTPLTLDGSYLLHQMFRVRWATWRALGAAEQ
                     EAVLDQATAVLHGMENNKQEATGLFAMLGHKGDLMLVHFRKALDALNLAELELAQLNL
                     LDFLEPTTSYLSVVELGLYEASVKLYAELTARGLKPGTAEWNREVEAELSKQRQAAAP
                     RLWPEIPQRRYLCFYPMNKFRGEARNWYNEPIRERQRMMREHGMIGRHYAGRVNQIIS
                     GSIGFDDWEWGVDLFADDPLVFKKLVYEMRFDEASAVYGLFGTFYVGLRFSADQLKNF
                     FHGKTPTFP"
     gene            3446..4207
                     /locus_tag="VF089_09395"
     CDS             3446..4207
                     /locus_tag="VF089_09395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6174217.1"
                     /translation="MRLHNKVVVITGAAHGIGKAYARRFAEEGAQVVIADIDSQGGHT
                     VAQALADSGFPAWARATDVRNYSDVQGLMRATADKFGRVDVLLNNAAIYVTQNLWKGP
                     VEDLELAEWDRVIEVNLKGVFLCCKAAIPFMKRQRSGKIINIASGTFFSGSGNMPHYT
                     TAKGGIVALTRVMARQLGEWGINVNCMTPGSTMSEDSVTDEVLKRREGSVDKRAFRRI
                     ETPADIVGTAVFLASSDSDFITGQLLVVEGGGIMH"
     gene            complement(4254..4763)
                     /locus_tag="VF089_09400"
     CDS             complement(4254..4763)
                     /locus_tag="VF089_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX6174218.1"
                     /translation="MENLVDFYSLLGIKRNASEAEIKQAYRKMAFRYHPDRNPDDKQA
                     AETFTQVLQAYGILSDSSKRSIYDRATRPPGEEEQQEEKPQAEQFGDHIHQGFNHSYD
                     FKAKGESQAKTEPQPKCPQCSTLGTDYIVSRKGGAGSSRGKQFVLAPFTVVFCSECGH
                     VYGIIGQSA"
     gene            complement(4817..5236)
                     /locus_tag="VF089_09405"
     CDS             complement(4817..5236)
                     /locus_tag="VF089_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174219.1"
                     /translation="MRTLDIISDTSGYIVLAGYQPPPAPMPVSLQYTPADILELDRAG
                     HKQRGTSSATKEFVSLVQILRAIGGYLDKNQARLIRISNNEAPGTSPYFSVEYETKEG
                     ERVVDDRDGAAIYDMCVSMYKQRRKVDHSAKLPSHGR"
     gene            complement(5472..>5885)
                     /locus_tag="VF089_09410"
     CDS             complement(5472..>5885)
                     /locus_tag="VF089_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174220.1"
                     /translation="RAIGQDLQGHGALVYEINVSDAEYRVQCDCDLPPPSNLLSLCYT
                     SRILEQIDFMGKLKRTGVSSVNSPESLADTLRSVGAYVDRENGRILRIANYEFLGPEI
                     AYRVQFALPDGSRVENTLSKPALDEITKSLRQKRK"
BASE COUNT         1370 a         1553 c         1644 g         1318 t
ORIGIN      
        1 attcttggac agcggcaaaa gaattttgtg aggacgcggc gagcaggaac agagaaaaaa
       61 ccgagctata gagccaacgc tgcatcctgt taccgtaaag gaagtcagct ttaacttcaa
      121 ggaatcgcgg cagcacagca atgtccgcgt catgggtgcg aacatggatg cgcggtgcgg
      181 atcaaagtca aagcagaaag atgattctgt cggtcgaaaa cagcgagcga ggatttcgtc
      241 tcgtaagtac ttcagtatgc caaagtcgaa cttgagactc atttgactag ctctgatcac
      301 cacaatagta tgaccgcaga cgaggaggac cccatgaccg atgtgaccga catgagagga
      361 tacgtggagt tgctggagga gatgggcgaa ctgcgaagac tcgagggcgt cgatcttaac
      421 ttggaagtcg gcgcgctcac ggagcgggcg gcggagaaag aaggcccggc gctgctgttt
      481 agcaatttca aagaatatcc agcgggtttt cgcgtcatat ccaatgtttt ccgaacctgc
      541 aggcgcaccg gaccggcgat gggcatcgat cccgagctta agggcgtcga ttacctgaac
      601 gcgtggcgga aaaaattagc tgcgtacaaa ccggtgccgg tccaacaagt cgaaggcggt
      661 ccggtgtttg aaaaccaaat ggttgatgat caggtcgatc tatataaatt tcccgtgccc
      721 aaatggcacg atctcgatgg cggcccttat atcggtaccg gctgtggagt gatcaccaaa
      781 gacccggaaa ccggcaagat caatatcggc acgtaccgcg tgatgatcca gaataagaat
      841 actctttcgg tgaagatgaa catgggcaag cacggccgat tggcgtttga acgcgcccgc
      901 gatgctggca agcccttacc gatcgccatt acattctgcc agggaccggc gatctttctg
      961 gcagctcaga tgccgctgcc gcccgacgtc aatgaattcg aatttgccgg ttatcttcag
     1021 ggcgcaccgg taccggtggc gcgcggcgcg gcaaccgggc tgccgattcc ggccaacgcg
     1081 gaaatcgtcg ttgaaggcga actcccgcca atgaaggacc aagacatgcc caaggaaggg
     1141 cccttcggcg aatggccggg ttacttcacc gaagagaatg tcggtgaaac gccgatcatg
     1201 aacgttaaac ggatctatta tcgaaatgat cctatcatcc tcggcgcgcc gccgctcaag
     1261 ccgccggcaa gttacttgcc cattccattg ggcgcggcga cgctctggga acaattggac
     1321 aaagcgggta ttccggaggt caaaggcgtt tggggttttg tttatggcgg ccaacctggc
     1381 ccttttacca tcatatccat caagcagcgt tatgccggcc actcgaaaca ggctctgttg
     1441 gttgccgccg gcgcccgcgc cggagcctac ggcggaaaat tcgtcgtcgt ggtcgacgat
     1501 gacatcgata tcgccaatcc cgccgatgtg atttgggcaa tcgctacccg ttgtcacgtt
     1561 cgcgaaggcc ttgatgtcgt caagggagtt tgggcctccg tatgtgagcc ggccttgcct
     1621 cccgaggaaa gatcaccgcg cggttacacc tctgaccggg tgctgatcga tgcctgccgg
     1681 ccgtttaaat ggatggatca atttccgccg gtcaacgcgt ttaccagcga cttcaaagat
     1741 caaatcgaga gtaaatttaa aatctagaat tcaaaaacct cttgcgacga tcttaggtcc
     1801 acccgatcaa ccggcctcct gccaagccgt aggattgcgt tatgggcagt atcgaagcgt
     1861 tgagcctggc aataggaaca gcttggactt ccggcatcaa cctgtatgcc acggtggcgg
     1921 ctttggggct ggccggccac ttcggaatga tccaattgcc acaaagccta gaggtgctaa
     1981 ctaatcccct cgtcatcgca gtggcgtgca tcatgtatgc gatcgaattt tttgccgata
     2041 aaattcccta tgtcgacaac ggttgggacg tattacacac gtttattcga gtgcctgccg
     2101 gcgccatcct agcggcgcgt tcactgggtg acatgagtcc ggctttggaa acggccgccc
     2161 tgctggctgg aggcagcatc gctttggcag cgcatggcac caaagcgacc agccgtttag
     2221 cgattaactc cagccctgaa ccgtttagca actggattgc cagcttcgtt gaggacatcg
     2281 cggttttggg cggcatttgg ataatcttca atcatccatg gctgatgctt gcgttggttg
     2341 ttgttttcct ggcggtgatg atttggattg cgccaaagat ctttcgcctt tctaagcgtg
     2401 ggtttaccat gctgtgcaag cggctgggcg gtagcaaaca ggagcaagcg accacgagca
     2461 cgcctcagag cccttaacac gagagcacaa ttcattatca ctgcagccga cgagcacatg
     2521 taaactcgag gatatggaac cacacgctga gaaacgagca ggcgatctgc ccccaacacc
     2581 gctgactctc gacggctctt atctactaca tcaaatgttt cgcgtgcgct gggccacttg
     2641 gcgcgcgctc ggcgctgccg agcaagaggc ggttctcgat caagccacgg cggtacttca
     2701 tggcatggag aacaacaagc aggaagccac cggcttgttc gccatgctcg gacacaaagg
     2761 cgacctgatg ttggtgcatt ttcgcaaagc tctcgatgcc ttgaacctcg ccgaactcga
     2821 gctcgcgcaa ttaaacctgc tcgattttct cgagccgaca acatcctatc tttcggtcgt
     2881 cgagcttgga ctttatgaag cttcggtgaa gctgtatgcc gagctgaccg cccgaggctt
     2941 aaagcccggc acggcggagt ggaatcggga agtggaagct gaactatcca agcagcggca
     3001 ggctgcggcg ccgcggctat ggccggaaat tccgcagcgc cgctatttgt gcttttaccc
     3061 gatgaacaaa tttcgcggcg aagccagaaa ttggtacaac gagccgatcc gcgagcgtca
     3121 acgaatgatg cgcgagcacg gcatgatcgg acgtcattat gccggccgcg tcaatcaaat
     3181 catttcgggc tccatcggct ttgatgattg ggaatgggga gtcgatctgt tcgccgatga
     3241 tccgctggtt tttaaaaaac ttgtatacga gatgcgtttc gatgaagcca gcgcggtgta
     3301 cggattgttc ggcacgttct acgtcggcct gcgtttttct gccgatcaat tgaaaaactt
     3361 cttccacggc aaaacgccta catttcccta agccagttcg cgtttgcgac atcgaatggt
     3421 ttgatacgtg accgcctgag gcgacatgag attacacaac aaagtcgtgg ttattaccgg
     3481 cgccgcgcac ggcattggca aagcctatgc gcgcaggttt gccgaagaag gcgcccaggt
     3541 ggtaattgcc gacatcgata gccaaggcgg ccatacagtc gcccaagcct tggcggattc
     3601 agggttcccc gcatgggcgc gcgcaaccga cgtcagaaac tattcagatg tgcaaggttt
     3661 aatgcgcgcg acggccgata agttcggccg agtcgatgtg ctcctcaaca acgccgcgat
     3721 ctacgtcacg cagaaccttt ggaagggtcc ggtggaagat ctcgaattgg ctgagtggga
     3781 ccgggtcatt gaggtaaact tgaaaggcgt tttcctatgc tgcaaagcgg cgatcccatt
     3841 catgaagcgt cagcgcagcg gcaagattat caatattgct tccggcacgt ttttcagcgg
     3901 ttccggcaac atgccgcatt atacaactgc gaaaggcggc atcgtcgcgc taacgcgagt
     3961 catggcacga cagctcggag aatggggtat caatgtcaat tgcatgacac ctggctctac
     4021 catgagcgag gattcggtca cggacgaagt gctcaagcgg cgtgagggca gtgtggataa
     4081 acgcgctttc cgtcgcatcg aaacaccggc cgacatcgtc gggaccgcgg tatttttggc
     4141 tagttcggac agtgatttca taaccggaca gttactggta gtggaaggtg gcggaattat
     4201 gcattgaacc aaagcgttcg atcgccaatc gatagcgcgc ggcccaacca atctcaagcc
     4261 gactggccaa taataccgta gacgtgtccg cactcgctac agaaaacaac ggtgaacggc
     4321 gcaagcacga attgtttgcc gcgcgacgac ccggcgccgc ctttacgcga gacgatgtaa
     4381 tccgtgccga gcgtggaaca ttgcggacat ttaggttgcg gttcagtctt ggcctgtgac
     4441 tcaccctttg ccttgaagtc gtacgagtgg ttgaatcctt gatggatgtg atcgccaaac
     4501 tgctcggcct gtggcttttc ttcctgctgc tcttcttcac caggcggacg tgtcgcccgg
     4561 tcgtagatac tacgcttgct gctgtcggat agaatgccat atgcctgtag cacttgggtg
     4621 aatgtctccg ccgcctgttt atcatcggga ttgcgatcag ggtgatagcg aaatgccatc
     4681 ttgcggtatg cttgtttgat ttcggcttcg ctggcgtttc gtttaatccc cagcaaggag
     4741 taaaaatcaa ctagattctc caaattcgcc tcacaaaaaa ttcttgcccg ccgggccatc
     4801 actgagtgat ggaatcttag cgtccgtggc tggggagctt cgccgaatga tcaactttac
     4861 gacgctgttt gtacatgctg acgcacatgt cataaatggc agcaccatct cgatcgtcaa
     4921 ctactcgctc gccctccttg gtctcatatt cgactgaaaa ataagggctg gtgccaggcg
     4981 cttcgttgtt tgaaatgcgg atgagccggg cttggttttt atcgaggtat ccgccaatgg
     5041 ctcggagaat ctggacaaga ctaacgaact cttttgtcgc tgaagatgtg ccgcgctgct
     5101 tatggccggc gcgatcgagc tcgaggatat cagccggcgt gtattgaagg cttaccggca
     5161 taggtgccgg cggaggttgg taaccagcca gcacgatgta cccggaagta tccgaaatga
     5221 tatcaagcgt ccgcaaacct cggccttcga gatcctgacc aatacatcga agagcggttg
     5281 aataattaac gggatggagc cgtgccagcc ttctcatacc ttgccagaaa atcgatataa
     5341 cgaactcatc atcgagaacg gaaatggtac gaatagtgtc cgaaaaaatc aaccgaattc
     5401 ttggatgtca ggtggacctc tcctgccaag gaatcatcac ccgctgagaa acatattcat
     5461 ttagctcgcg ctcactttct cttttggcgc agggacttag tgatttcatc gagggctggt
     5521 tttgacagag tgttctccac ccggcttcca tcgggcaagg caaattgaac ccggtaagcg
     5581 atctcgggtc ctagaaattc gtaattagca atacgcagga tacggccgtt ctcccgatct
     5641 acataggctc ccacgctgcg gagagtgtca gcaagcgact ccgggctgtt cacagaggaa
     5701 acgccggtgc gtttcaattt gcccatgaag tctatttgct cgagaattct gctcgtataa
     5761 caaagagaca gaagattact tggcggcggc agatcgcagt cacactggac gcggtactcg
     5821 gcgtcgctga cgttgatctc atagaccagc gcgccatggc cctgcaaatc ctgaccgatg
     5881 gcgcg
//
LOCUS       DASUTG010000412         9303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2140487, whole genome shotgun sequence.
ACCESSION   DASUTG010000412 DASUTG010000000
VERSION     DASUTG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140487"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1009
                     /locus_tag="VF089_09415"
     CDS             35..1009
                     /locus_tag="VF089_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174221.1"
                     /translation="MSRLWIAFLLLLSTSDVTAQPLTRVMSGYAATSGPHAVLWLARE
                     AGLFEKNGLRTDIAYIRSGSTMGQALVAGEIQLAQMGGPAALAAGVAGFDVTMVAVAL
                     NTTPIVIMGKVSKMEELKGKAIGVTRYGSNTDISARFAIRKAGMQPEKDVALIQLEDY
                     AGIMGGIQSGRIAAGALADPFTDHAKKLGYKEIADIAALGLEFPFVGIVTKKTFIKEQ
                     PDLVQRFVRAYTESIALYKNNRELAKQVTSKYTGIKDPDILTSTVDFYAPKLASVPYP
                     TIGGIRFVLDQVATRDPRAKNFAPETFMDLRFVKQLEDSGFIQGLNRK"
     gene            complement(1198..1695)
                     /locus_tag="VF089_09420"
     CDS             complement(1198..1695)
                     /locus_tag="VF089_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX6174222.1"
                     /translation="MTRLHSVSLLLHLIALAMWLGGIAFFLIVFGPAVHGLKAGLGLT
                     LLNRGRMTFELLSWTAIGLLFVTGILNLVLRSQMTGVHLGQYYLTILTVKLLLVVAML
                     VHHALQVFKYGPKIAANTNQAAITADSWPEALRADWQKWFMLLKINATIGPIATLLGL
                     ALVRR"
     gene            1981..3009
                     /locus_tag="VF089_09425"
     CDS             1981..3009
                     /locus_tag="VF089_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174223.1"
                     /translation="MRLIGQAAVRKFLNCIMLSVVVAFDSSPGNAQTIEETYQRALKE
                     GGTLNVYGTLTPATAAKVLPLFEKRFAGLKTVNTGASSDKIVARAISEARGGRTIGDV
                     FHMNLENIMQLHEQGLVLEKLPAEADVFPNHLKGSYWVATRFNTLVAAWNTQLVSKSD
                     EPRSFEEFADPKWKGKLLAEPRDAEFLIAFAEQRYRSSDKAVELFKKIAANNVEFHSG
                     HPQLAELLTAGQGAVCLTCYTHHLLPRMQKGAPLNYMRSFGVGLISATAVFKNAPHPN
                     TAWLFHRWAASDEGQKAFGIEGRVPANPKVEPAEAIGLKTVYGLGEKEYKEFSRYEQL
                     WKQIFSLR"
     gene            complement(3041..4366)
                     /locus_tag="VF089_09430"
     CDS             complement(3041..4366)
                     /locus_tag="VF089_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX6174224.1"
                     /translation="MHCITLESQYGVPTVGVHAKPFASVVRSVAYTKGMPHQRFAFVP
                     MPVMGKTASELKAYIDGNDPVTGKPVMEEIVEALTKPLSDQEKQKITIDRSTPRLIHP
                     NSESNLQRLFLDNGWTDHLPIVVPTEERVAAMLSGTRRGADEVVGTMRPTRTREGWQF
                     TVEKVAVNAVMAGAKPEYLPVVLALAASEVTARPSTTTSGATMAVVNGPIRHQIGMNW
                     GIGAMGPYNHANATIGRAYGLLSQNLQGGSVPGETYMGSQGNNYAYNSITYAENEERS
                     PWTPLHVQHGFTTSDSVVSLFRHCRTTSFQMGLREKHWRDHLQYLLRGLNPHTPPTLV
                     LDPIVARQFIERCGFDTKEKLIHWIYEHATMPAGIYWDDQLIQNYIHPRALKGEEPYA
                     TKLKASADELVPMFRLDEIEVVVVGGETNGYWRVMGCNYDKSVSIDRWR"
     gene            complement(4391..4720)
                     /locus_tag="VF089_09435"
     CDS             complement(4391..4720)
                     /locus_tag="VF089_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174225.1"
                     /translation="MNQGETVRPVASAKEKVTALNPMGYPPTIEQLGMAPRFESLDGK
                     TIYLVDVHFVDGDKLLQQMQAWFAEHLPKARIVLASKAGVYTEDDPKLFSEIRENDAA
                     AIMAVGH"
     gene            5104..5769
                     /gene="coxB"
                     /locus_tag="VF089_09440"
     CDS             5104..5769
                     /gene="coxB"
                     /locus_tag="VF089_09440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6174226.1"
                     /translation="MMRWLPEDVSTYGHEIDSLFFLIYYITAATFILVTVLMVLFLVM
                     YRERPGRRATYSHGNTTLEIIWTIIPAAILIVLSFMSVNTWAKLKNTVPPSDFAIQVT
                     AKQFNWGIAYPGADGKLGTDDDVKFDNDVHVPVNKVVHVHLGSEDVIHSFFIPNLRFK
                     QDMVPGRTVTGWFEATKPGRYELPCAELCGFGHSGMKGWLHVHSAEDYAKWAAENLTA
                     KQQ"
     gene            5830..7581
                     /locus_tag="VF089_09445"
     CDS             5830..7581
                     /locus_tag="VF089_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6174227.1"
                     /translation="MSQTVAAGAGHVAHAHHEELGFWRTYIFSMDHKMIGRQFLFMGL
                     LMLIIGGLLAMMMRWQLAWPETAVPGLSAIPEPYMYEGIIPPQTYNALFTMHATIMIF
                     FVVMPIMVGCYGNFLIPLMIGTRDMAFPLLNMLSFWVGAVAGVIMLASFLVPGGPAAA
                     GWTGYATLSARAAYTGVDLGQDLWIISLLVLGISSLMGSINYITTIVNMRAPGMTYFR
                     MPLTVWSLFITAILLLLALPVLTAALAMLLFDRTLGTSFFLPDGGGQPLLWQHMFWFF
                     GHPEVYILVLPAMGVTSDLLSTFSRKPIFGYHAMAFSMIAIAFLSWIVWGHHMFVSGM
                     NPLLGTAFMMTTMVIAVPSAIKVFNWLGTLWGGSIRLTSPMLFALGFVSNFLIGGLSG
                     IFMASTPVDIFIHDTYYIVAHFHYVVAGIIFALFAAVYYWFPKMFSRMMSEPLGKIHF
                     VLTYIFFNLTFFPMHFLGVGGHMRRIYNPMQYEFIQPMQDWNVFITVSALLLGLSQIP
                     FLINFFWSLFAGKKAERNPWQANTLEWTAPTPPPHGNFEVQPTVYRGPYEYSSPEVKE
                     DWLPQDKPLAKTAARAH"
     gene            7597..8541
                     /locus_tag="VF089_09450"
     CDS             7597..8541
                     /locus_tag="VF089_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX6174228.1"
                     /translation="MRAEGAGNGAEVSVWPHRLAVITACATFPLLFIGGLVTSKGAGL
                     AVPDWPTTFGYNMFLYPWSKMVGNIFYEHSHRLVASAVGFLTIALALSLWLQERRKWL
                     RWLGAIALVLVVVQGVLGGLRVTLLEHSLAIVHASLAQAFFALTVSLTLFTSPEWREK
                     IAEPPLADYGRLRRLAAVTTGLIYLQVLFGAVLRHTGLRLDAHLLFAALVTLHVVLLT
                     ARIFKERRNHRRLTRPALFLSVMLLLQLVLGTVSYLAKFTTILRISSDVTVYLTTTHL
                     AVGALMLVTSLIVTLRCYRYPTVRRAAGQEVFIEQYSQ"
     gene            8538..>9303
                     /gene="cyoE"
                     /locus_tag="VF089_09455"
     CDS             8538..>9303
                     /gene="cyoE"
                     /locus_tag="VF089_09455"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6174229.1"
                     /translation="MTSNTQTFDLPTARVIRRVADFCELAKPRVVLMVLITAFVGFYV
                     GSADVPDYSQLMKMLVGTALAAGGTLALNQLIERRSDALMTRTRHRPIPDGRVTATEA
                     FWFGLAVTLAGLAYLAIAVNLISAGITALITVTYLLFYTPMKRKSPLCLIVGAVPGAL
                     PPVIGWVAARGTLDVDAWVLFAILFLWQVPHTLAIAWLYREDYAKAGIQFLPVIEPGG
                     SSANRQIISHCAALLAVSLLPTLLGLAGAVYFMVAFV"
BASE COUNT         1982 a         2501 c         2505 g         2315 t
ORIGIN      
        1 tagagacctg agaatcaagt aagggagggg caaaatgagc cgactctgga tagcattttt
       61 gttgctgcta tcgaccagtg atgtcacagc gcaaccatta actcgggtga tgtcgggtta
      121 cgctgccaca tcgggaccgc atgcagtcct ttggctggct cgtgaagccg gtctgttcga
      181 aaaaaatggt ttgcgaacgg acatcgctta catacgcagt ggctcgacaa tgggccaggc
      241 cttagtcgcc ggagaaatcc aactagcgca aatgggcggt ccggccgctc tcgcggcagg
      301 tgtcgccggc tttgacgtca cgatggtcgc cgttgcactg aatacaacgc ccatcgtgat
      361 catgggcaaa gtttccaaaa tggaagagct caagggcaaa gcaatcgggg tgacgcgcta
      421 cggttccaac accgatatct ctgctcggtt cgcgatcaga aaagccggaa tgcaaccgga
      481 aaaagatgtg gctctaatcc agttggaaga ctacgcgggg attatgggcg ggattcagtc
      541 cgggcggatt gccgccggcg cgctggctga tccgtttacg gatcacgcca agaagttggg
      601 ctataaggaa atcgccgaca ttgcagcgct gggcctggag tttccgtttg tcgggattgt
      661 caccaagaaa actttcatca aggaacagcc ggatcttgtc cagagattcg tgcgcgccta
      721 taccgagtct atcgccctct ataaaaacaa tcgcgagctg gcgaagcagg taaccagcaa
      781 atacaccggc atcaaagatc cagatattct gacctccacg gtggattttt atgcgcccaa
      841 gctagccagc gtgccctatc cgacaatcgg cggaatacga tttgtcttgg atcaggtcgc
      901 gactcgcgat ccccgcgcga agaactttgc tcctgagact ttcatggatc tgcggtttgt
      961 aaagcaactc gaggacagcg ggttcatcca gggattgaac cggaagtagc gaaccgattt
     1021 gacgaaagat gtgacagttg aagatatcag ccggagtaaa taggatagaa ctttcaccac
     1081 ggaagtcacg aagagcacga agcttaagaa tatccgctac ccgtcaaacc cttcgtgacc
     1141 tgcggttcgt ggtgagttcg taatttgtag agcgcccgtg atactcgcat accgcgtcta
     1201 acgcctgacg agtgccaggc ccagcaaggt tgcgatcggg ccgatcgtag cgttgatctt
     1261 tagcagcata aaccattttt gccagtccgc gcgtaacgct tccggccacg aatctgcggt
     1321 gattgcggcc tgattggtat tggcggcgat ctttgggccg tatttaaaaa cttgcagcgc
     1381 atgatgcacc agcatcgcca caacgagtag tagcttgact gtaagaattg tgaggtagta
     1441 ctgccccaga tgaacgccgg tcatctggct gcgcaggacc aggttcagta tcccggtaac
     1501 gaataacagc ccgatcgctg tccaggacag caattcaaag gtcatcctcc cgcggttcaa
     1561 aagtgttaaa cccagtcccg cctttaggcc gtgtaccgcc ggtccaaaaa cgatcagaaa
     1621 gaaagctatt ccgccgagcc acatcgccag cgcaatcaga tgcagcagga ggcttacaga
     1681 gtgtaatcgg gtcatggatt gaactagcga tcagcagtcg cactgtgatt aacatcgctc
     1741 cctaggcttt gcaaacctta tggcgccgag tggtggcgcc tgcaatctgg cggttatctt
     1801 cgaaatcacg cagccagtct ttgcttccat atttaagttc aaaattcacc tggtgttaga
     1861 ttacgatcat aaaccacgat gtcctcttaa cgagggggcc atatgatgtt ctttatcttg
     1921 acaatttgaa acaccctctg ttacgaagtc tcattcttta aatttaaata ggagacgcca
     1981 atgcggctaa tcggccaagc agctgtccgg aaattcttga attgcatcat gctttctgtg
     2041 gtggtggcgt tcgattcgtc gccgggcaat gctcagacta tcgaagagac ctatcagcgg
     2101 gcgttgaagg agggcggcac actcaacgtc tacggaactt taacgccagc tactgcggct
     2161 aaagttctgc cgctattcga gaaacgtttt gccggactca aaacggtcaa caccggtgct
     2221 agttccgaca aaatcgtcgc ccgggcgatt tcggaggcgc gcggtggcag aaccattggc
     2281 gatgtttttc acatgaacct ggaaaacatc atgcagctcc atgaacaagg tttggtgctc
     2341 gagaagctgc cggcggaagc cgacgtgttt ccgaatcatc tcaaaggttc ctattgggtc
     2401 gccacccgtt tcaacactct ggtggcggcc tggaatacgc aactcgtcag taaatcggac
     2461 gagcctagga gttttgaaga atttgctgac ccgaaatgga aaggcaagct gctagcggag
     2521 ccgcgtgatg ccgaattttt gattgccttt gccgaacaga gatatagaag tagcgacaaa
     2581 gccgtcgagt tgttcaaaaa gatcgccgcc aacaatgtcg agtttcatag cggtcacccg
     2641 caattggcgg aactgctgac ggccgggcag ggcgcggtct gcttgacctg ctacacccat
     2701 catctgttgc cgagaatgca gaagggagcg ccattaaatt acatgcgcag ttttggcgtc
     2761 ggtttgatca gcgccaccgc cgtcttcaag aatgcgccgc acccaaacac cgcttggtta
     2821 tttcaccgct gggccgcctc tgatgaaggg caaaaggcat tcggcatcga agggcgggtg
     2881 ccggcgaatc cgaaagtgga gcccgccgag gctatcggac tcaaaaccgt ctatggattg
     2941 ggcgagaaag aatacaaaga gttttccagg tacgaacagc tttggaagca gatcttcagc
     3001 ttgcgttagc gttttcccat cggacatgtg atccgccccg ctatctccat cgatcgatgg
     3061 agacgctctt gtcgtagttg caacccatca ctcgccagta gccgttggtt tcgccgccga
     3121 ccaccaccac ctcgatctca tccaagcgaa acatcggcac gagttcatcg gcagaggctt
     3181 tgagcttcgt cgcatagggc tcttcgccct tgagcgctct cggatgaata tagttttgga
     3241 tgagttgatc gtcccaataa atgcccgccg gcatagtggc atgctcgtag atccagtgaa
     3301 ttaacttttc cttagtatcg aagccgcacc gttcgatgaa ttgccgcgct acgatgggat
     3361 ccagtacgag ggtgggcggt gtatgtgggt ttaaacctcg cagcagatac tgaagatgat
     3421 cacgccaatg tttttctcga agccccatct ggaacgacgt cgtgcggcaa tgcctgaata
     3481 agctgacaac gctgtcgctg gtcgtaaagc catgctggac gtggagcggt gtccagggac
     3541 ttctttcctc attttccgcg tacgtgatgc tgttataagc gtagttgtta ccttgagagc
     3601 ccatgtaggt ttcgcccggc accgagccgc cttgcagatt ctgcgataac agtccgtaag
     3661 ccctgccgat ggtggcatta gcgtggttgt aaggtcccat tgcgcctatg ccccagttca
     3721 taccgatctg atgtcggatc ggtccattca ctaccgccat ggtggcgccg gaagtcgtgg
     3781 tgctcggccg cgccgtgact tctgaggccg caagcgccag gacgaccggg aggtactcgg
     3841 gtttggcgcc ggccatcacc gcgttcaccg caaccttctc caccgtgaac tgccacccct
     3901 cacgggtgcg tgtcgggcgc atcgtgccca ccacctcatc ggcgccgcgg cgggtgccgg
     3961 acagcatcgc cgcaactctt tcttcagtcg gaacgacgat cggcaaatgg tcggtccaac
     4021 cgttatccaa aaacaaacgc tgcagattac tctcgctatt ggggtggatc agtctcggcg
     4081 ttgaccgatc gatcgtgatc ttctgcttct cctgatcgct taacggcttt gtcagcgcct
     4141 caacgatttc ctccatcaca ggtttgccgg ttaccggatc attgccatcg atgtaggctt
     4201 tcagttctga ggcggttttg cccatcacag gcatcggcac aaaggcgaac ctctgatgcg
     4261 gcattccttt ggtataagca acggatctta cgaccgaagc gaaaggtttg gcgtggacgc
     4321 ccacggttgg cacgccatat tggctttcca atgtaataca gtgcacggcg accgccggcg
     4381 cgcaggtact tcaatgccct actgccataa tggcggcggc atcgttctcg cggatttcgc
     4441 taaagagctt tggatcatct tccgtgtaaa caccggcttt gctcgccaag actattctcg
     4501 ctttgggcaa atgttcggcg aaccacgcct gcatctgttg caacaatttg tcgccatcga
     4561 cgaaatggac atcgaccaaa taaatcgttt tgccgtctag actctcgaac cgtggcgcca
     4621 tacccagctg ttcgatggtc ggcgggtatc ccatcgggtt taaagcggta actttttcct
     4681 tggcagatgc aaccggccga actgtttcac cttgattcat ttgcaatctc tcctgctatc
     4741 gatgaactat ttcgccgatg agacaatgaa ctgctcgtgt cgaagtccct ctcactcacg
     4801 cttttatgcc agcgtcggat cgatggcaat accgaattgg gtcaaccatg ggcgcgatat
     4861 agggttccag cggttgtggt gctttaaccc gtgtttagac aatccgtttg acgtgttcca
     4921 tctttggttg cttatcctcg gatgattcgc atgaactgac atggatccga gaaatttgct
     4981 gcgccgaagt ggcattgacg ccgcgatatg gggcggtttt ccccttgaaa tggccaaggg
     5041 gctatgttag ctaaattgaa gtttttctgc ccattcccca aacacaagga tggatcgcta
     5101 gagatgatgc gctggttgcc agaggatgta tcgacctatg gtcacgaaat cgactcgtta
     5161 ttttttctta tctattacat tacggccgcc acttttatct tggtcacggt gctgatggtt
     5221 ctgttcttgg tgatgtaccg ggagcggccc ggccggcgcg caacctatag tcacggcaac
     5281 acgacgcttg agatcatttg gactattatt ccagccgcta tcttaatcgt tctctcgttc
     5341 atgagcgtga acacctgggc gaagttgaaa aataccgtgc cgcccagcga ttttgccatc
     5401 caagttacgg ccaaacagtt taactggggc atcgcttacc cgggagccga cggcaaacta
     5461 ggcaccgatg atgacgtcaa attcgacaac gatgttcatg tgccggtcaa taaggtcgtc
     5521 cacgtccatc tcggatcgga agatgtgatc cacagctttt tcattcccaa tctcagattc
     5581 aagcaagata tggtgccagg acggacagtg acgggttggt ttgaagcgac gaaacccggc
     5641 aggtatgaac tcccgtgcgc ggaattgtgc ggcttcggcc actccggtat gaagggctgg
     5701 ctgcacgtcc acagcgccga agattacgcc aagtgggccg cggaaaattt aaccgccaag
     5761 cagcagtgac acttcccatt ctaaaaactt aggttcatcc gaagcagatt cacggaggat
     5821 tataacacga tgagtcagac agtagcggcc ggggccggcc acgtagcgca cgcgcatcac
     5881 gaagagcttg gtttttggcg cacctacatt ttttccatgg atcacaagat gatcggccgg
     5941 cagtttttat ttatgggcct gttgatgttg atcataggtg gattgctggc gatgatgatg
     6001 cgctggcagt tggcctggcc ggaaaccgcc gttcccggat tgagcgctat tcctgagccc
     6061 tacatgtacg aaggcatcat tccgccgcag acctacaatg cgctctttac catgcacgcc
     6121 accatcatga tcttcttcgt cgtcatgccg attatggtcg gctgctacgg caattttctt
     6181 atcccgttga tgatcggcac gcgcgacatg gcttttccgc tcttgaacat gctttctttc
     6241 tgggtcggcg ccgtcgccgg tgtcatcatg ttagcaagct tcttagtacc gggagggccg
     6301 gccgcggccg gatggaccgg ctacgcaacc ctctctgcga gggccgcata cacaggcgtg
     6361 gatttaggtc aggatctctg gatcatcagt ttgctcgtgc tcggcatctc ttctttgatg
     6421 ggctcgatca attacatcac caccatcgtc aacatgcggg cgccgggaat gacctacttc
     6481 cgcatgccat tgacggtgtg gtcgcttttc atcaccgcca tcctgctttt gttggctttg
     6541 cccgtcctga ccgccgcgct ggccatgctg ctcttcgatc ggacgcttgg cacgagcttc
     6601 ttccttcccg acggcggtgg gcagccgctt ctctggcagc atatgttttg gtttttcggc
     6661 catccagagg tctacatcct ggttcttcct gccatgggag tgacttccga tttgctttcg
     6721 actttctcac gcaagcctat ctttggctat catgcgatgg cgttctcaat gatcgcgatc
     6781 gctttcctct cctggatcgt ttggggccat catatgttcg tcagcggcat gaacccgctg
     6841 ctcggcaccg ccttcatgat gacgacaatg gtgatcgcgg ttccatcagc gatcaaagta
     6901 tttaactggc tcggaactct ctggggtgga agtattcggc taacttcccc gatgctgttt
     6961 gccctcggtt tcgtgtctaa cttcctgatc ggcggtttga gcggcatctt catggcttca
     7021 accccggtcg atattttcat ccacgacacc tattatatcg ttgctcactt tcactatgtc
     7081 gttgcgggaa ttatcttcgc gctgtttgcc gccgtctact actggtttcc caaaatgttt
     7141 agccggatga tgagcgagcc tctggggaag attcattttg tcctgaccta catattcttt
     7201 aacttaacct tcttcccgat gcattttctc ggcgtcggcg ggcatatgcg gcgcatctat
     7261 aatccgatgc aatacgagtt tatccagccg atgcaggact ggaatgtctt cattaccgtc
     7321 agcgcgctgc tgctgggcct ttctcagata ccgttcctga tcaatttctt ctggagtctt
     7381 tttgccggaa agaaagcaga gcgcaatcct tggcaagcca acaccttgga gtggaccgca
     7441 ccgacaccac cgccgcacgg taactttgaa gttcagccga cggtttaccg cggcccctac
     7501 gagtatagct cgcccgaggt caaagaagat tggctgccgc aggacaaacc gcttgccaaa
     7561 acggcggctc gagcgcatta gaaaaaaggg gaaaggatga gagctgaagg cgcaggcaac
     7621 ggcgcagagg tttcggtgtg gccccatcgg ctggccgtga taacggcttg cgcgacattt
     7681 ccgttgttgt ttatcggcgg cctagttacc agcaagggcg caggtcttgc ggtccctgat
     7741 tggcccacca ccttcggcta caacatgttt ctctatccct ggtcgaagat ggtcggcaat
     7801 attttctatg agcacagtca ccgtctggtc gcctcagcag tagggttctt gaccattgcg
     7861 ctagccttat cgttatggtt acaggagcgc cgcaaatggt tgcgttggct gggagcaatc
     7921 gcgctcgtgc tggttgtcgt acaaggcgtc ctcggcggct taagggttac cttgttagaa
     7981 cactccctgg cgattgttca cgcctcattg gctcaagctt tctttgcgct cacggtgagc
     8041 ctaactctat ttacctctcc agaatggcgg gagaagatcg cggaaccgcc gctagcggat
     8101 tatggacgct tgcgccgact cgcggccgtc acaaccggac tcatctacct gcaagtgttg
     8161 ttcggggcgg tgttgcgcca tactggcctg cgactcgatg ctcatttgtt gtttgccgcg
     8221 ctggtaacgt tgcacgtggt gcttctcacc gcgagaattt tcaaagagcg caggaatcat
     8281 cgacggctta cccgtccggc gctgtttcta agcgtaatgt tgttgctgca actagttttg
     8341 gggacggtct cgtatctcgc taaatttact accatactaa gaatatccag cgatgtgacg
     8401 gtgtatctta ccacgacgca tttggccgtc ggcgctctta tgcttgtaac aagcctgata
     8461 gtcacgttac ggtgttatcg gtacccaacc gtacggaggg ccgcaggaca agaagttttt
     8521 atcgagcaat attcacaatg acctcgaaca cgcaaacttt cgacttgcca accgcgcggg
     8581 ttattcgccg ggtagcggat ttttgcgagc tcgccaagcc gcgggtcgtg ctgatggtgt
     8641 tgattaccgc gtttgtcggt ttttacgtcg gctcggcgga tgtgccggat tattcacagc
     8701 tgatgaaaat gctcgtgggc actgctttgg ctgccggcgg cacattggcg ctgaatcagt
     8761 taatcgagcg tcgcagcgac gctctgatga cgcggacgcg ccaccggcca attccggacg
     8821 gccgagtcac ggcaacggaa gctttttggt ttggtttggc tgtgactcta gccggtcttg
     8881 cgtatctggc tatagccgtc aatctcatca gtgcagggat taccgcgctg atcacagtga
     8941 cttacttgct cttttatacg ccgatgaagc gaaagagccc attgtgcttg atcgtcggcg
     9001 cagtccccgg cgctctaccc ccggtaattg gctgggtagc cgcccgaggc accttggatg
     9061 tcgacgcttg ggtcttgttt gcaattctgt ttctctggca ggtgccgcac acacttgcga
     9121 ttgcctggtt gtaccgtgaa gattatgcca aggcgggaat ccaattcctg ccggtcattg
     9181 aaccgggagg ctccagcgcg aatcgccaga tcatttctca ctgcgccgct ttgctggccg
     9241 tcagtttgtt gcccaccctt ttaggtttag cgggtgcagt ttattttatg gtcgcgtttg
     9301 ttc
//
LOCUS       DASUTG010000413         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_64589, whole genome shotgun sequence.
ACCESSION   DASUTG010000413 DASUTG010000000
VERSION     DASUTG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64589"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VF089_09460"
     CDS             complement(<1..857)
                     /locus_tag="VF089_09460"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX6174230.1"
                     /translation="MRIAVMGTGAVGGYFGAKLTAAGNDVVFITRPRHLPALRRNGLR
                     IESSTGNVYVRNALFTDSPADAGMVDLVLFCVKSYDTTEAAAALAPLITKGTAILSLQ
                     NGIDNPEKIAGFWGTERTLAGVVYIGAEVSAPGVIQHSSGGKIVLGPMSGHPAETAQS
                     VERTLARAAIPCAISSAIEQVQWAKLLWNAPFCAISCLTRSTVHEIIESEALTSLVRD
                     CMKEVRAAARTRGIDLKTELFDQTLAFSEGLGDFKPSMLQDLEAGKPLECDALNGFVV
                     RLLEQAGERA"
     gene            963..1175
                     /locus_tag="VF089_09465"
     CDS             963..1175
                     /locus_tag="VF089_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174231.1"
                     /translation="MKTSAMEKIDPQYDHVKLEQYCPGQQVIFRGKPFTIQRRTTLAS
                     GEPAVVLQGDKEQFVISANQFFAGVK"
     gene            1221..2213
                     /locus_tag="VF089_09470"
     CDS             1221..2213
                     /locus_tag="VF089_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6174232.1"
                     /translation="MGKIRLTLACWDYDRTRALQDGRVEVEGIDLTYLPLRVEETFWR
                     MLRYGEFDVAEMSTGSYLMSRDKGAPKLIAIPVFPSKTFRHSCIYINTDSGIRKPADM
                     AGKRVGVPEYQITMATWARGILQHEYGVLPEKMKWFTGGEEHPGREDKVRHNLPANID
                     IRPIPAGNTLSGMLATGEIDAMISAHMPSPFVRGHPKVQRLIPNFREVEQDYFRRTKI
                     FPIMHTVVIREEVYEKNPWIAQSLFKAFNESKKLCQESMYEFSALKYMLAWSIDEMEK
                     EREVLGDDPWAYGLEANRHVLETLVQYTYEQGLISKPLDVDSLFAKSTLEEFKI"
BASE COUNT          570 a          620 c          641 g          502 t
ORIGIN      
        1 gctcgctctc cggcttgctc taataagcgc acgacaaatc cgttgagggc atcgcattca
       61 agcggcttgc ctgcttccag atcctgcagc atcgacggtt tgaaatcgcc caatccctcg
      121 gaaaacgcca atgtctgatc aaacagttcc gtcttcaggt cgatgccgcg tgtacgcgcc
      181 gcagctcgga cctctttcat gcagtcccgg acgagactgg tgagagcttc agattcgatg
      241 atctcatgaa ccgtggagcg ggttaagcac gaaatagcgc aaaacggcgc gttccacagc
      301 agctttgccc actgaacttg ctcgatggcg gagctgattg cgcatgggat cgccgcgcgt
      361 gccaacgttc gctcgacgct ttgagcggtc tcggcgggat gaccactcat gggccccaga
      421 acgatctttc ccccggacga atgctggatc acaccagggg ccgagacctc agcaccaata
      481 taaacgacgc ctgccaaagt tctttcggtg ccccaaaatc cagctatttt ttcgggatta
      541 tcgatgccat tctgaagcga gagaatggcc gtgcctttgg tgatcagcgg cgccaacgcc
      601 gctgccgcct cagttgtgtc gtaggacttg acgcagaaga gcaccaaatc aaccatgccg
      661 gcatcagcag gcgagtcggt gaacaacgca ttgcgcacgt atacattccc agttgagctt
      721 tcgatgcgca atccattgcg acgcagtgct ggcagatgtc tcggtcgggt gatgaatacg
      781 acatcattgc cggctgccgt aagtttcgca ccgaaatatc cgcccaccgc gcctgtgccc
      841 atgaccgcaa ttcgcatgtt tgtctctggc gatgtccgac tgtattctcg gctgggactg
      901 atgtcaattt cacacacgca gtaactaaat cgacgcgatt acgctttgat cgtggatgtg
      961 ttatgaaaac ctctgcgatg gaaaagattg atccgcagta cgaccatgtg aaacttgagc
     1021 agtattgccc cgggcagcaa gttatcttca gaggaaaacc ctttactatc cagcggcgga
     1081 cgacccttgc ttcgggcgaa ccggcagtcg tgctgcaggg cgacaaagag cagttcgtca
     1141 tcagtgcgaa tcaattcttt gccggagtga agtaactcgt cgcgcaaagt caatcatgat
     1201 ctagtgaata ggagaacaga atgggaaaaa tccgcttgac actggcctgt tgggactatg
     1261 atcgcacccg cgcgctacaa gacggcagag tcgaagtcga aggcatagat ttgacctatt
     1321 tgccgttgcg agttgaagag accttttggc gaatgctgcg ttacggggaa ttcgatgtgg
     1381 cggaaatgtc gacgggttct taccttatgt cgcgggacaa aggcgcgcct aaattgattg
     1441 ctattccggt ctttccgtcc aagaccttcc gccactcctg tatctatatc aacactgatt
     1501 ccggcatcag gaaaccggcc gatatggcgg ggaaaagagt cggggtaccc gagtatcaga
     1561 tcaccatggc tacctgggcg cggggtatcc tgcaacatga atacggagtt cttccggaaa
     1621 agatgaaatg gtttaccgga ggcgaagaac accccggccg agaggataag gtcaggcata
     1681 acttgccagc caatatcgat atccgaccga ttccggcggg gaacacgctc tcaggcatgt
     1741 tggcaacagg cgagatcgat gcgatgatct cagcccatat gccctcgccg tttgtacgag
     1801 gccatcccaa agtgcagcgg ctgataccga attttcgcga ggtcgagcaa gactacttcc
     1861 gccgcaccaa gatctttccg atcatgcaca ccgtggtgat tcgcgaggaa gtttacgaaa
     1921 aaaatccttg gatcgcgcaa agcctgttca aggcattcaa tgaatccaag aagctatgcc
     1981 aggagagcat gtacgaattc tccgcgctta aatatatgct ggcatggtcc atcgatgaga
     2041 tggaaaagga gcgcgaggtt ctcggagatg atccctgggc gtacggcttg gaagccaatc
     2101 gccatgtcct ggagacgctt gtccaataca cgtacgagca gggtttgatc agcaagcctc
     2161 tggatgttga ctcgcttttt gccaaaagta cgctggagga attcaaaatc taagagccgc
     2221 agtaggacgg cgacccctat atggccgacg aaaagaacga gacccttgaa gaaagcaaat
     2281 ggtggatcga tcgcggcccg gtgaaggagc gcgaaaagcc gctggcgccg gtc
//
LOCUS       DASUTG010000414         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_213010, whole genome shotgun sequence.
ACCESSION   DASUTG010000414 DASUTG010000000
VERSION     DASUTG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213010"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VF089_09475"
     CDS             complement(<1..900)
                     /locus_tag="VF089_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEX6174233.1"
                     /translation="MIISRTPFRISFFGGGTDYPAWYRDHGGTVLGATIDKYCYLTCR
                     YLPPFFKHRIRVVYSQIENCQMVDEIAHPAVREVLRYLQIDRGVEIHHDGDLPARSGM
                     GSSSAFTVGLLHALYALKGQMPTKRQLAAESIQIEQEMIKETVGSQDQVLAAFGGFNH
                     VVFSQDGEITVKPITLSGDRLRELSSHLMLFYTGIERTASDVADSYVADVDGKRSQLR
                     IMKGLVEESLAVLNNGRDIAAFGALLDEGWKAKRSLSTRVSNSYVDEHYAHAISNGAL
                     GGKLLGAGGGGFMLLFVPPNKQNQ"
     gene            complement(897..1454)
                     /gene="gmhB"
                     /locus_tag="VF089_09480"
     CDS             complement(897..1454)
                     /gene="gmhB"
                     /locus_tag="VF089_09480"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX6174234.1"
                     /translation="MRRRFVILDRDGTIIEEVSYLSDPKQIALIPGAGKALRDLSNMG
                     FGLVVITNQSGVGRGYFDEAQLTRIHDRLLEMLRAEEITLDGLYFCPHTPQDLCSCRK
                     PHTGLIQKASEDLGFDLSGSIVIGDKASDIEMGSRVGALTVLVRTGYGAQVASERKVA
                     PDYIVDDLPAAVEVIGRSFRKVRDQ"
     gene            complement(1426..2178)
                     /locus_tag="VF089_09485"
     CDS             complement(1426..2178)
                     /locus_tag="VF089_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6174235.1"
                     /translation="MVQDVSAFSDLNVAVLAGGLGTRLLPVVSDRPKALAEIHGRPFL
                     QYLLDQLSTAGCSRVVLCTGHLGEQIERAFGKRYRNLQISYSREARPLGTGGALRLAL
                     PGLVSDPVLVMNGDSFCATDWRSLWDWHCRHGSQATMLLVEVPNTERYGSVKINADGA
                     VTEFVEKKPGGSGLINAGLYLLSRQVIDSIPEGTAVSLEHDIFPALMSHGLYGYQERG
                     RFLDIGTPEDFAAAEEFFAAINQRETAVCNTR"
     gene            complement(2209..3147)
                     /locus_tag="VF089_09490"
     CDS             complement(2209..3147)
                     /locus_tag="VF089_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174236.1"
                     /translation="MPTHVVVTGAAGYLGSVLCEHLLAAGYRVTAADGLLYRQRSLFH
                     LCANPQFDFVVGDARDEQLMRRLVKDADVLIPLAAIVGAPACDRDPWLARSVNLEAIR
                     LLARLRSNDQLIVYPTTNSGYGTKSGDFFCTEDTPLEPISLYGQTKTEAEAEILERTN
                     TVTLRLATVFGMSPRMRLDLLVNHFVYAAITDGYIVIFEKDFKRNYIHIRDVADCFIH
                     CIENGKKMAGRPYNAGLDSANLSKEALALKIKEYVPNFYIHFGQVGTDPDKRNYIVSN
                     QRLRDAGFEAQRSLDSGIQELLKGYRMMGRGEFANI"
BASE COUNT          849 a          924 c          823 g          772 t
ORIGIN      
        1 ttgattctgt ttgtttggag gcacaaacag gagcataaac cccccgccgc cggctccgag
       61 caactttccc ccgagagcgc cgtttgagat tgcgtgcgca taatgctcat cgacgtagct
      121 gtttgaaact ctcgtgctta gactgcgctt ggctttccat ccctcgtcga ggagggcgcc
      181 aaatgcagct atatcgcgtc cattgttcaa tactgcgagg ctttcttcga ccagaccttt
      241 catgatgcgc aactggcttc tttttccgtc gacatcggca acgtaactgt ccgcgacgtc
      301 ggaggcggtg cgctctatgc cggtatagaa aagcattaga tgagagctta gctcgcgtag
      361 gcgatcccct gatagggtaa tcggtttgac ggtaatttca ccatcttggg aaaaaaccac
      421 atgattgaat ccgccaaatg ccgccaaaac ctgatcctgg gagccgacgg tttccttgat
      481 catctcctgt tcgatctgaa tactttccgc cgccagttgt cgcttagtcg gcatctgtcc
      541 tttgagagcg taaagggcgt gcaacaaacc caccgtaaag gcagagctcg agcccatgcc
      601 gctgcgggcc ggaaggtcac catcgtggtg aatctccaca cctcgatcga tttgtaagta
      661 acgcagcact tcgcgcacgg cgggatgagc gatttcatcg accatctggc agttttcaat
      721 ctgggaataa acaacacgaa tcctatgttt aaaaaacggc ggaagataac ggcaggtgag
      781 atagcagtat ttgtcgatgg ttgcccccag cacggtgccg ccgtggtcgc ggtaccatgc
      841 aggataatcg gtgccaccac caaaaaaaga aatacgaaac ggcgtcctgc tgatgatcat
      901 tgatcacgta cctttctgaa actcctgccg atgacttcca cggctgcggg caaatcatca
      961 acaatgtagt ccggcgcaac cttccgctca gaagctactt gagccccgta gcccgtccgt
     1021 accagcacgg ttaaggctcc aacccgggaa cccatttcga tatcgcttgc cttgtcacca
     1081 ataacaatgc tgcctgacaa atcaaagccc aaatcttccg atgccttttg gatcaatcca
     1141 gtatgcggtt tgcggcacga acataggtct tgaggagtat gcgggcagaa gtaaagacca
     1201 tcgagagtta tctcttcagc acgcagcatc tccaataacc tgtcatgaat ccgcgtcaac
     1261 tgcgcttcgt cgaaatatcc ccgtccaacc cccgattgat tggtgatgac aaccagaccg
     1321 aagcccatat tgctgagatc cctgagcgcc ttgccggccc cagggatcag cgcaatctgt
     1381 ttcggatccg aaaggtaaga cacctcttcg atgatcgtac cgtccctatc gagtattaca
     1441 aaccgccgtc tcacgttggt taatcgctgc aaaaaattct tcggcggcag caaaatcctc
     1501 cggcgtgccg atatcgagaa aacgccctcg ctcctgataa ccataaagcc catggctcat
     1561 caaggctgga aaaatgtcat gttccaatga aacagccgta ccttcgggga tcgagtcgat
     1621 aacctgacgg ctaagcaaat atagtccggc attgatcaag cctgaaccgc ctggcttttt
     1681 ttcgacaaat tcggtcacag cgccgtcagc gttgatcttt accgagccgt aacgttccgt
     1741 gttcggcacc tccactaaca acatcgtcgc ttgagaaccg tgacggcagt gccaatccca
     1801 caaactcctc caatccgtgg cgcaaaacga atctccgttc atgaccagaa ccggatcgga
     1861 cactagaccc ggtaaagcca atcgcagagc gccccccgtg cctaaaggcc gcgcttcccg
     1921 cgaatatgag atttgcaagt ttcgatatct tttgccgaat gctcgctcga tctgttctcc
     1981 gagatgtccg gtacagagca caactcgaga acatcctgcg gttgataact gatccagcaa
     2041 atattgcaaa aaagggcgtc catgaatctc cgccagtgct ttgggccggt ctgagacgac
     2101 tggaagcaat cttgttccta atccacccgc gagcactgcg acattgagat ctgaaaaagc
     2161 actgacgtcc tgtaccacaa tggtttctcg cagctgttaa aacaaaatct aaatattagc
     2221 gaactcaccg cggcccatca tgcgatagcc ttttagtaac tcctggatac ctgagtccag
     2281 tgagcgctga gcctcgaagc cagcatctcg caggcgttgg ttcgagacga tgtaattgcg
     2341 cttatcggga tcggttccga cctggccgaa gtggatgtaa aaattaggca cgtactcttt
     2401 gatcttcaaa gccaaggctt ctttggacag attggcgcta tcgagtcccg cattgtaagg
     2461 gcggcccgcc attttcttgc cgttttctat acaatgaata aagcagtcgg cgacgtcgcg
     2521 tatatggatg tagtttcgtt tgaaatcttt ttcaaaaata acaatgtagc catcggtaat
     2581 ggcagcatag acaaaatgat tgacaagcag atcaaggcgc atgcgcggcg acatgccgaa
     2641 aaccgtcgca aggcgcaagg tgaccgtatt ggtcctttcc aagatttccg cctcggcctc
     2701 ggttttggtt tggccataga gcgaaatagg ctcgagcgga gtgtcctcgg tacaaaagaa
     2761 atctcccgat ttggtgccat agccgctgtt ggtggtagga taaacgatca actgatcgtt
     2821 gctgcgcaaa cgcgcgagca gtctaattgc ttcaagattg accgaccggg ccaaccaggg
     2881 atcacgatcg cacgccggtg ccccgacgat cgccgcaaga ggaatgagca cgtccgcatc
     2941 tttgaccagg cggcgcatca actgctcatc gcgcgcatct ccgacgacga agtcgaattg
     3001 agggttggcg cagagatgaa acaagctcct ttggcgataa aggagcccat ccgctgcggt
     3061 cactcgatat cccgccgcga gcaaatgctc gcacaaaacg gatcccaaat agccggcagc
     3121 gccggtgacg acgacatgag ttggcattat tgattgaaga gtcctttgat taacgaggtg
     3181 ctaactcgct ttcatagcac attccaataa gacattggaa ccgatggaaa agttttttat
     3241 ctgcggaaag gacggtccaa ccaatttaga acgcttctta gcgatcacct tttttgagat
     3301 caatcagatt ccgctccaaa ccatagccga attaccggag aggaggcgag tcgataccca
     3361 tcagtttc
//
LOCUS       DASUTG010000415         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_508733, whole genome shotgun sequence.
ACCESSION   DASUTG010000415 DASUTG010000000
VERSION     DASUTG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508733"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF089_09495"
     CDS             <1..538
                     /locus_tag="VF089_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEX6174237.1"
                     /translation="AAITFADTGHLCMGTLHSTNVNQSFERILNFFPAERHSQILMHL
                     SLSLRAVISQRLIPSVDGARVAAIEILIDTPRIKDLIKQGDIDSLKEAMEQGIQEGCQ
                     TFDQALFTLYKENKITLQQALMNSDSANNLRLKIKLEGLTGDDALDKLLDKGSAYSNK
                     AGLQIQGNQRIGSTFRRI"
     gene            782..1306
                     /locus_tag="VF089_09500"
     CDS             782..1306
                     /locus_tag="VF089_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174238.1"
                     /translation="MADQTVYALLLRPVGQLLEALRIESFSLSVENDDLVVRDKTPGR
                     KQITPREKALLAEWQVTHTRPQDKEGALKLASGILEWRMTPADIDRLEREGQSRRRNP
                     NLTPDIHSVSQMLRVVGALVDHKNGRLLQLTKEDQKLTFEYELLSGKKVAEELGVPAL
                     YDLWVRIYRRQTPR"
     gene            1477..1932
                     /locus_tag="VF089_09505"
     CDS             1477..1932
                     /locus_tag="VF089_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174239.1"
                     /translation="MESFSLAMEGDTVIVRGRKRRDQAVDNLSRRGFWQLLRSNRTAE
                     VDEWESVELRYTPEQLSHMDDEGRSKRGNLGSPDAHSASQIVRAVGALVDQRQARLMS
                     VRKDEEKIEIEYESPLKGRVTEDFTVATLYEYWVRMYLKRSNRQEQSSS"
     gene            complement(1984..2739)
                     /locus_tag="VF089_09510"
     CDS             complement(1984..2739)
                     /locus_tag="VF089_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX6174240.1"
                     /translation="MAAQEVHMQVWRGDASGGEFRDYRVETGEGMVVLDVIHAIQAQH
                     ANDLAVRWNCKAGKCGSCSAEINGKPRLMCMTRMNTFQPGETITVSPIKTFPIIRDLV
                     TDVSYNYEKARQVPAFKPKPKEADGTRRMFQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HEENKQQFAGPRLFVRLAALEMHPLDTNDRIELIKQKAGIGFCNITKCCTEVCPEHIH
                     ITDNAIIPLKERIVDRYFDPIAKLLGLFSEKTR"
     gene            complement(2740..4542)
                     /locus_tag="VF089_09515"
     CDS             complement(2740..4542)
                     /locus_tag="VF089_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX6174241.1"
                     /translation="MVEEKYETHDYDVVIIGAGGAGLRAAVEASAQGARTALVCKSLL
                     GKAHTVMAEGGIAAAMGNVYPEDNWKVHFRDTMRGGKLLNNWRMAQIHAQESPDRVLE
                     LEEWGALFDRTHDGLILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHKGIDVYME
                     CNLQRLLTDGGKISGAFGYWRETGKFIVFKCKAVVLATGGGGKAWKVTSNSWEYTGDG
                     VTMAMDVGADLMDMEFMQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNTKNERFMFNY
                     IPEFFRAETAETEKEADAWYDDKKNNRRTPDLLPRDEVARAINSEVKAGRGTPHGGVF
                     LDIASRRPADYIQRRLPSMYHQFKELADVDITKEPMEVGPTCHYSMGGVRVDADSTAT
                     TVPGLFAAGEVAAGLHGANRLGGNSLSDLLVFGRRAGLYAAEYAKANKGTVNVDVGQI
                     DLYAKELLSPFESKGKENPYSVHADLQECMQNLVGIIRTESELKQALDIIAQLKERAR
                     NLKVEGNRQYNTGWHLALDLHSLLSFAEAATIAAIERKESRGGHTRDDYPNTDAKFAK
                     TNIVIRRKNGELTASQEPLPEMPDELKVLLEEKK"
     gene            complement(4542..5306)
                     /locus_tag="VF089_09520"
     CDS             complement(4542..5306)
                     /locus_tag="VF089_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174242.1"
                     /translation="MSDKTLMLDQFRTLRKDTWWKEPLMVVLVLGGFAIYATWAALQN
                     AHYYVAPYLSPFYSPCIAENCAHVSVPLIGSWWNLSPAFLILWIPGGFRATCYYYRKA
                     YYRSFFLSPPACAVRDASKSYSGETRFPFLLQNLHRYFFYLSILILAFLWWDTVLAFQ
                     FADGIGMGVGTVVILINAFLLTLFSLSCNSCRHVCGGYVDSFHSAPTRYKVWSFISRL
                     NGRHMEYAWVSLIWVALTDLYVRLLSMGVIQDLRFF"
     gene            complement(5674..>6423)
                     /locus_tag="VF089_09525"
     CDS             complement(5674..>6423)
                     /locus_tag="VF089_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX6174243.1"
                     /translation="AKGLQELALGGTAVGTGINTHPQFAARAIEIISAETGLPFRETE
                     NHFEAQAARDASVHVSGALKTLAVSLIKIANDLRWLASGPRCAIGEIGLPDTQPGSSI
                     MPGKVNPVLCESVIQVAAHVIGCDATITLCGQTGNFELNVTMPIVTLKMLESIEFTSN
                     VINAFTEKCIIGIEANEARCRELVEKSLAMVTALAPLIGYDAAAAIAHEAVASGKSVR
                     EVAQSKDLLTPEQLDKALEPWSMTMPGREKE"
BASE COUNT         1497 a         1693 c         1695 g         1538 t
ORIGIN      
        1 ggcggcgatc actttcgccg ataccggtca tctttgtatg ggcacgcttc attcaaccaa
       61 cgtcaatcaa tcgtttgaac gtatccttaa tttcttcccg gcggaacggc actcacagat
      121 tctcatgcat ctttctttga gtttacgtgc cgtcatttcg cagcgtttga taccttcggt
      181 ggatggagcg cgggtggcgg caatcgagat tttgatcgat accccgcgga ttaaagatct
      241 gattaagcaa ggagacattg acagccttaa agaggccatg gaacagggaa ttcaggaggg
      301 ttgccaaacg ttcgaccaag cgctgttcac tctatacaag gaaaacaaaa tcactctcca
      361 gcaagcgttg atgaattcag atagcgcgaa taacctgcgc cttaagatca aattggaggg
      421 gttaaccggc gacgatgctc tcgacaagtt gctagacaag ggaagtgcct actccaacaa
      481 ggcgggattg cagattcaag gtaatcaacg aatcggcagt actttccgta ggatttagtg
      541 cccggccatg caatggctcc gaagtagatc tgccttgact taggaaaagg cgacacgtta
      601 gataagctgt ttttgcttct tagagttgta tagcaggact gtagagggag acatcatagc
      661 tgttgtgagg acgcgagctt cagagctcga gtttcgctgt cgacgattaa gcggccgtcg
      721 actcgaaaca aacgcaattc tctctagcat caatgacaag gagaaatgat acttagtacc
      781 catggctgat caaacagttt acgctctttt acttcgccct gtggggcaac tgctcgaggc
      841 gctgcggatc gaatcatttt ctttgtccgt cgagaacgac gatttagtgg tgcgtgacaa
      901 aacccccggg cgcaagcaga tcacgcctcg cgaaaaggct cttctcgcgg aatggcaggt
      961 aacgcatact cgtccacaag acaaggaagg cgcgttaaaa ttggccagtg gcattctgga
     1021 gtggcgcatg accccggccg acattgaccg gctcgagcga gaggggcaat cacggcggcg
     1081 caatcctaat ctgactccgg acattcactc agtctcgcag atgctgcggg tggtcggcgc
     1141 gctcgtcgat cataaaaacg gaaggctttt gcagcttacc aaggaagacc aaaagctgac
     1201 gttcgaatat gaactcttaa gcggtaagaa agtcgcggaa gaattgggag tgccggcgct
     1261 ttacgatctc tgggtccgta tctatcgaag acagactcct agatagctaa gcactgatta
     1321 gacgggttct ttattgagga ttggagcggg gcgactcagc gccgagccaa aatttcactt
     1381 caggtaatac atggagacag gagttccgca ctgaatacca gccatagtta tacacagtta
     1441 cttcgtccgg taggtcaagc gctgcagtca ctccaaatgg aatctttttc attggcgatg
     1501 gaaggcgaca ccgttatcgt tcggggacgc aagcgcaggg atcaagcggt tgataatttg
     1561 tcgcgccggg ggttctggca actacttcga tccaatcgaa ctgccgaggt tgacgaatgg
     1621 gagtcggttg agttgcgcta tacgcccgag cagttgagtc atatggacga tgaggggcga
     1681 tcgaagcgcg gtaatctcgg cagcccagat gctcattcgg cctcgcagat cgttcgcgct
     1741 gtgggagccc ttgtcgatca aagacaagcg cggctcatgt ccgtccggaa ggacgaagaa
     1801 aaaatcgaga tcgaatacga gtcaccgttg aagggcaggg taaccgagga tttcacggtc
     1861 gcgaccctat acgagtattg ggttaggatg tatttgaaac gtagtaatcg tcaagagcag
     1921 agcagcagct gatcgaggat tccaagtcat cgcaagcaga gtagggaatc tatgacgccc
     1981 acgtcatctc gtcttttcag aaaacagccc caatagtttc gcaatcggat caaaataccg
     2041 gtcgacgatg cgttctttca aaggaatgat ggcattgtcc gtgatatgta tgtgttcagg
     2101 acacacctca gtacagcatt tggtgatatt acagaaaccg atccccgcct tctgcttgat
     2161 caactcgatg cgatcattgg tgtcgagcgg atgcatctcc aacgccgcca ggcgcacaaa
     2221 aagtcgtggt ccggcaaact gttgcttatt ctcctcgtga tcgcgaatga cgtgacagac
     2281 gttttgacat agaaaacact cgatgcattt atggaattct tgtacgcggt cgatgtcttc
     2341 ttgaaacatg cgtcgggtgc catcggcttc cttcggtttc ggcttgaagg ccggcacctg
     2401 acgcgccttt tcataattgt acgagacgtc ggtgaccagg tcacggatga tcggaaacgt
     2461 tttgatcggt gagaccgtga tcgtttcgcc gggctgaaac gtattcattc gagtcatgca
     2521 catgagccgc ggcttgccat tgatctcggc gctgcaggat ccgcatttcc cagctttgca
     2581 attccagcga accgccaaat catttgcatg ttgcgcctga atggcgtgga tgacgtctag
     2641 cacgaccatg ccttccccgg tctccactcg ataatctctg aattcgccgc cgctggcgtc
     2701 gccgcgccat acttgcatat gcacttcttg tgcagccatt tactttttct cctcaagcaa
     2761 tactttcagt tcatcgggca tctccggaag cggctcttgc gatgctgtca gctcgccgtt
     2821 ctttcgtcgg atgacgatat tagttttggc gaacttcgca tcggtattcg gataatcgtc
     2881 tcgggtatgt cccccgcggc tttctttacg ctcgattgcg gcaatcgttg ccgcttctgc
     2941 aaacgagagc agagaatgaa gatcgagcgc gagatgccag ccggtattgt attgccgatt
     3001 gccttccact ttaagatttc gcgcgcgttc tttcagttgg gcgataatat cgagagcttg
     3061 cttaagctcc gactcggtac ggatgattcc taccaaattc tgcatgcact cctgtaagtc
     3121 tgcatgtacc gaatacgggt tttccttgcc cttactttca aagggggaca atagctcttt
     3181 ggcatataga tcaatttggc cgacatcgac attaaccgtg cctttattgg ccttggcgta
     3241 ttccgcggcg tacagaccgg cgcgccggcc gaacaccaac agatccgaaa gggaattgcc
     3301 gcctagacgg tttgcgccgt gtaatccggc ggcgacttca ccggcagcga acaggcccgg
     3361 cacagtagtc gcggtactgt ctgcatctac tctcaccccg cccatactgt agtggcaagt
     3421 cgggcccact tccatgggct ccttggtgat gtcgacatcg gcgagttcct tgaactgatg
     3481 atacatagac ggcagccggc gctggatgta gtccgctggt cggcgcgagg cgatgtctaa
     3541 aaacacaccg ccatgaggtg tgccgcggcc tgctttgacc tcagagttga tcgcgcgtgc
     3601 gacctcgtct cgaggtagta agtccggagt tcgccggttg ttcttcttgt cgtcatacca
     3661 agcatccgct tccttttccg tctccgccgt ctcggcgcga aaaaactctg ggatgtagtt
     3721 aaacatgaac cgctcatttt tggtgttctt gagcgttccg ccatcgcccc gcacgccctc
     3781 ggtcactagg atcccgcgga cgctgggcgg ccaaaccatg cccgtaggat gaaattgcat
     3841 aaactccatg tccatcaagt cagcgcctac gtccatggcc atggtaaccc cgtcgccggt
     3901 atactcccag gagttcgacg tgaccttcca ggctttgcca ccgccgccgg ttgcgagcac
     3961 gacggcctta catttaaaaa cgatgaattt gccggtttcc ctccagtagc cgaaggcgcc
     4021 gctgatcttt ccgccgtctg tcagtaatcg ctggaggttg cactccatgt agacgtcgat
     4081 gcccttgtga accgcgtgct gttgaagcgt gcggatcatt tcgaggccgg tgcggtcgcc
     4141 gacatgcgcc agccgagcgt atcggtgtcc gccgaagtcg cgctgcaaaa ttagaccatc
     4201 atgggtccgg tcgaacagcg caccccattc ttcgagttcg agtacgcggt ccggcgattc
     4261 ttgcgcgtgg atctgcgcca tccgccaatt gttgagaagc ttgccgccgc gcattgtatc
     4321 gcgaaaatga accttccagt tgtcttccgg ataaacgttg cccatcgccg cggcgatgcc
     4381 tccttcggcc ataacggtgt gagccttgcc caatagtgat ttgcagacca gggcggttct
     4441 ggctccttgt gccgaagcct ctactgcggc gcgaaggccg gcgcctcccg cgccgatgat
     4501 gactacatcg tagtcgtggg tttcgtactt ttcttcaacc attagaagaa cctcaaatcc
     4561 tgaatgacgc ccatcgacag cagccggacg taaagatcgg tcaacgcgac ccaaatcaag
     4621 ctcacccagg cgtactccat atggcgcccg ttaagccggc ttataaaact ccaaacctta
     4681 tatcgcgtgg gcgcactgtg aaatgaatca acgtacccgc cgcagacatg ccgacatgag
     4741 ttgcaagaaa gactgaacag ggtcagcaga aaagcgttga taaggatgac gacggtcccc
     4801 acacccatgc cgataccatc agcaaactga aatgccaaaa ccgtgtccca ccagagaaaa
     4861 gctaagatca gaatcgatag gtagaagaag tatcggtgaa gattttgcag caggaacgga
     4921 aagcgggttt ctccgctata agattttgag gcatcccgca cggcgcaagc cggaggcgac
     4981 aagaaaaaag acctgtaata agctttgcgg tagtagtagc aggtggcgcg aaatccgccc
     5041 ggaatccata ggattaagaa tgccggcgag agattccacc acgatcctat aagcggaacc
     5101 gaaacgtgag cgcagttttc cgcgatgcag ggcgagtaga acggcgacaa atagggcgcg
     5161 acatagtaat gtgcattctg caaagccgcc caggtggcgt agatggcgaa accgccgagc
     5221 actagaacca ccatcagcgg ctctttccac caggtatctt tacgtaacgt ccggaattgg
     5281 tctagcatta aagttttatc gctcacgttt gggtcctttg actttactgc tcattattta
     5341 tttggctgag cggttgccgg aaaatcgcgc aagctgaaga ttttcaaacg atgcattcga
     5401 ttacgccaat acggttcccg cttaaatcga aacaccggaa actgtggctc ttgcttccgc
     5461 ggaaatctag ggcaggcgat tgctgatgca caatggcact tacttggtcg cggcaattcc
     5521 ccttttgatt aatccgtttt ttctagtttg atgtagtttc aaacgatctg cggaacatcc
     5581 tctttgtgct ccttgaatcg ggaaactcct ttgcgatgac gaattattct cggcttataa
     5641 cagaaaaaaa aaagctttgc acgcgctgac ttgctactcc ttttctcttc ccggcatggt
     5701 catgctccag ggttccagag ctttgtcgag ttgctctgga gtaagcaggt cttttgactg
     5761 cgccacttct cgaaccgact ttccgcttgc aactgcttca tgcgcaatag ccgcggccgc
     5821 atcataaccg atcaacggag ccaatgctgt taccatggcg aggctttttt caaccagctc
     5881 cctacagcga gcttcattcg cttcgatgcc aataatgcat ttttcagtaa atgcattgat
     5941 cacgttactg gtaaattcaa tggactcgag cattttgagc gtaacaattg gcattgtcac
     6001 attcaattcg aaattcccgg tttggccgca gagcgtaatt gtagcgtcgc aacctatgac
     6061 gtgagcagcg acttggataa cggactcgca gagaaccgga ttgactttgc ccggcatgat
     6121 cgacgaaccc ggctgggtgt ccggtaggcc gatctcgccg atagcacagc gcggtcccga
     6181 agcaagccaa cgtagatcat tagctatttt gatcagactc accgccaaag tcttgagagc
     6241 gccgctgacg tgtactgagg catcgcgtgc cgcctgtgcc tcaaagtgat tctccgtctc
     6301 gcggaagggg aggccggttt ctgctgagat gatttcaatt gctcgggcgg caaattgcgg
     6361 gtgggtattg atgccggtcc ccacagccgt gcccccaagc gcaagttctt gcaggccttt
     6421 cgc
//
LOCUS       DASUTG010000416         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_213651, whole genome shotgun sequence.
ACCESSION   DASUTG010000416 DASUTG010000000
VERSION     DASUTG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213651"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF089_09530"
     CDS             <1..195
                     /locus_tag="VF089_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174244.1"
                     /translation="VILYAPLWVVERSLSVYWAQYCRVAHGGYPFGEQLLSKGTGRAW
                     RAARAFMRKPHPLTNPSRPG"
     gene            230..1720
                     /locus_tag="VF089_09535"
     CDS             230..1720
                     /locus_tag="VF089_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6174245.1"
                     /translation="MLDSNDARASNDLGDMPPAEFREALHRVADWVADYRERIEELRV
                     SPDIAPGEINARLPQAAPELAVSFSDIFRDFEELITPGIVHWGHPAFFGYFGSTTTSA
                     GILGEMLMGALNVSAMTWRTSPSATELETLVLDWLRQMLGLPTDYTGVVYDTASVAVM
                     HALAAAREDLGFAARARGLCGRPEIPVLRIYASDQAHSSVEKAAITLGLGEDNVRRVP
                     CEASYRMDVSALRSAIDTDLRGGLLPMAVVATVGTTSTTSVDPVDEIAVLCNEYRLWL
                     HVDAAYGGAMALLPEGRYVMKGVEAADSIVVNPHKWLFVPLDFSALYIRRPELLRATF
                     SLVPEYLRGDAEQAERNYMDYGIQLGRRFRALKAWMVIRSFGREGLATRIREHIRLAR
                     LFASWLDADSEFQVLAPVTMAVVCFRIAPHVPGTTPARDEEIDSLNRRLVEAVNRTGK
                     AYLTYTTLNGQVAMRLAVGNILTTERHLARVLDLIRRESAALRVAP"
     gene            2076..3071
                     /locus_tag="VF089_09540"
                     /pseudo
     CDS             2076..3071
                     /locus_tag="VF089_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            3114..3833
                     /locus_tag="VF089_09545"
     CDS             3114..3833
                     /locus_tag="VF089_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX6174246.1"
                     /translation="MSDEPVIEPSINYYQRLLAMDQDEAAEIVEEHLKSHPKEQIFDD
                     LLIPALNYAKRDFASGRMAENEQQFVLQATRQIIEDLDGFKTENESSPAPHAEMTTAV
                     TTQSLEGTPKVLIIGCPVRDEADELSLLMLRQSLDPLRYELEIIADELLAAEIIDQIA
                     EKKVDLVCLASLPPGGLAQTRYLCKRLRARFPESKLIVGRWGSRTDNSNALTDAGADR
                     TASKITETRDHIIQLSQILPP"
     gene            4155..>4405
                     /locus_tag="VF089_09550"
     CDS             4155..>4405
                     /locus_tag="VF089_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6174247.1"
                     /translation="MQSVYVAGIGMTKFGKDPRSLAEICHDAAQTALTSSEIQEVEAI
                     YIGVMNPEEFTGDSNIASHIADALGLTGIPAVRIETASSA"
BASE COUNT          908 a         1217 c         1244 g         1036 t
ORIGIN      
        1 gtgattctct atgcgcccct gtgggtagtg gagcgttcgc tgagcgtcta ttgggcccag
       61 tactgccgcg tggctcacgg cggttatccg ttcggggagc aattgctgtc taaggggacg
      121 gggcgagcat ggcgtgccgc gcgcgcgttt atgcgaaagc cgcatccttt gacgaatccg
      181 tcgcgcccgg gctaacccaa gcccgtgact aagatcaacg gagagaacga tgctcgacag
      241 caatgatgcg cgcgcttcaa acgatctcgg agatatgccg ccggcggaat tccgtgaagc
      301 tctgcatcgg gtcgcagatt gggtggcaga ctatcgcgag aggattgaag agctgagagt
      361 ttcgccagac attgcgcctg gtgagatcaa cgctcggctg ccgcaagcgg caccggagct
      421 tgccgtctcg ttcagcgata tctttcgaga ctttgaagaa ttaatcactc ccggcatcgt
      481 ccactggggg catcccgcgt tcttcggcta ttttggttcg accacaacct ctgccggaat
      541 tttgggtgaa atgctgatgg gagcactcaa cgtcagtgcg atgacctggc gcacctcgcc
      601 atcggcaacc gaactcgaaa cattggtgct cgactggctg cgtcagatgc ttgggcttcc
      661 cacagattac accggtgtgg tttacgacac cgcttcagta gctgtgatgc atgcgctggc
      721 ggcggcgcgc gaggatctcg gtttcgctgc ccgcgcgcgc ggtctgtgcg ggcggcctga
      781 gattcctgtg ttgcggattt acgcctcgga ccaggcgcat agctcagtag agaaagccgc
      841 tatcaccctg ggcctcggcg aggacaacgt gcgccgagtg ccatgcgagg cgagctatcg
      901 catggacgtc tctgcgctcc gcagcgccat cgacacggac ttacgcggcg gcctgctgcc
      961 gatggcagtc gttgccaccg tcggaaccac gtcgacaacc agcgtcgatc cagtggatga
     1021 aattgctgtt ctctgtaacg agtaccgttt atggcttcat gttgatgcgg cctacggggg
     1081 cgcgatggct ctcttgcccg aaggtcgtta tgtgatgaag ggcgtcgagg ccgccgactc
     1141 cattgtagtc aatccacata aatggttgtt tgtcccgctc gacttcagcg cgctttacat
     1201 aaggcggcca gagctcctac gcgccacctt ttcgctcgtc cccgaatact tgcggggcga
     1261 tgccgaacag gccgagcgca actacatgga ttacggcata caactcgggc gtcgctttcg
     1321 cgctctcaag gcgtggatgg tcatccgcag cttcggccgg gagggccttg ccactcggat
     1381 ccgggagcac attcgcctgg cgcgattgtt cgcatcctgg ctcgatgcgg attcggagtt
     1441 ccaggtgcta gcacccgtga caatggcggt tgtctgcttc cgcatagcgc cgcatgtgcc
     1501 tggaaccacg ccggcgcggg acgaagagat tgatagtctt aatcgaaggc tcgtagaagc
     1561 ggtcaatcgg accggcaaag cctatctgac ctacaccacc ctaaatggcc aggttgcaat
     1621 gcggctagcc gtcggcaaca tactaaccac tgagcgccac ctagcgcggg ttctcgacct
     1681 catcaggcgt gagtcagctg cgcttcgtgt tgccccttaa cgccacttgc ccttcgtaga
     1741 aaacgacgct cccactttgc atgccgctta cgtagtcgga gactttggcg catctgtcga
     1801 aataggcgct gtcatcgttt ttaccggggc aacgccctac ccgcggctca atccgcgatg
     1861 gattgatccg tcttgtgcgc acgaggtgcg tcctgcataa atcaatgaga ctcgacgagc
     1921 ctcgccgaaa atgtatgact gcttttaagg gcctctagga aggatgacgg ttgagcacgt
     1981 tcaataaaac gtcggatgca aagcctccga agccgcgttc tactcttgcg cctggtagcg
     2041 ccggccgcgg cggcaaatta ccatcggtac tgattttgcc aaccctcgtt ttgattatcg
     2101 ctacgctcta ttgggggcaa gcgtttttta tccccgttgc gctgtcaatc ttactgacgt
     2161 ttctactaac gccggttgcc gatgctctag agcgagtggg cttggggcgg gtggcttcgg
     2221 taatgattat cgtcgtcctt gcattctcac tgctggcggc aatcggctgg gtggttacac
     2281 ttcaactgac cagcgtcgct aacgagttgc ccacctatag aagtaatatc agacagaaaa
     2341 tcatcgacat acgcggcgcc ggtaaaggag gcgctctcga gaagatccgt gagactgccg
     2401 aagaggtaaa agaggagttt agcaaagacg agccggcatc ggcttcgtca aagccccgcg
     2461 aagttgtcgt tcaagccgag gagtcatcga cgttctggcc gctgccctta gcgaccgcgc
     2521 cgatgttgga acgattggct tcaggcggct tggtgatcgt gctggtgatt ttcatgctta
     2581 tccaacgcga aaacctgcgt aaccgtctga tccgtctggt cggctatggt cgtctgacaa
     2641 ttaccaccaa ggctctcgaa gaggcggcgc agtgaatcag tcgctatctg ctgatgcaat
     2701 ccatcatcaa tgcttcgttt ggaattacag tcggcatcgc gctctacctg ttcggccttc
     2761 cctatgcgct gctgtgggga tttttggctg cggtactgcg atttattcct tatgtcggtc
     2821 cggttgcggc agcgctcctg ccaagcgtcc ttagcatggc ggtctttccc ggttggactt
     2881 ggccgctctt ggtggtggca accatcattg ccttagaact catcaacaat atgattttgg
     2941 agccgctcct ctacggtgaa agcgcgggcg tttccgaggt cgggctcgtg atcgcggtcg
     3001 cgttctggac ttggctttgg ggacctatcg ggcttgttct ctcaactccg cttactgtct
     3061 gcgtcgtcgt tgtcagcaaa tatatgcctc agctggattt tatcggagtt ctgatgagcg
     3121 acgagccggt aatcgagcca agcatcaatt actaccagag gctgttggcc atggatcagg
     3181 acgaagcggc ggaaatcgtc gaagagcatc taaagtcgca tcctaaagag cagatctttg
     3241 acgacctgct gatccccgca ttgaattatg ccaagagaga ctttgcttcc ggtagaatgg
     3301 cggaaaacga gcaacaattt gttctgcaag cgacccgaca aataatcgaa gatctcgacg
     3361 gcttcaaaac ggaaaacgag tcctcgccgg caccgcacgc agaaatgaca acggcggtta
     3421 cgacgcaatc tctagagggc acgcccaagg tcctaatcat cggctgtccg gttcgcgacg
     3481 aagccgacga gttgtctttg ctgatgcttc gacagtcact cgatcctctc cgttatgaac
     3541 ttgagattat tgccgacgaa ctattggcgg ccgagatcat cgaccagatt gccgagaaaa
     3601 aggtggattt agtttgtctt gcgtcgttgc cgcctggcgg cttagctcag acgcgatacc
     3661 tttgcaaaag gttacgcgcg cgatttcccg agagcaaact catcgtcggc cgatggggta
     3721 gccgaaccga caatagcaac gcgctgaccg atgccggtgc tgacaggacc gcgagtaaga
     3781 ttactgaaac ccgggatcac atcatccaat taagccaaat tcttcctccc tgatggttct
     3841 caatcagttt attcgcggac tgctgatcac accggaaaaa gccatcgcta gccggtgagt
     3901 tccgcgacgt ccgagctggc ggggctgtct cactaggtgt gcgaaactta ctttgagaat
     3961 tccgcgacca ttgcattggc atttacgtca cgtcaatcgc gggacgcatc gtactctttg
     4021 gctgggtcac ccggcataac aaaatcttca tttcatattg atggattgtt tttcctccca
     4081 acacgtcagt ctttaaccgt tcgccctaac cactcacttg gaattagcgc cttggtcagt
     4141 ataattgtgt ttgtatgcaa tcggtttatg ttgccggaat cggcatgaca aagttcggca
     4201 aggaccctag gtctttggcc gagatttgcc atgatgcggc tcaaacagcg ctaacctctt
     4261 ccgaaataca ggaagtggaa gccatttata ttggcgtcat gaacccggag gaattcaccg
     4321 gcgacagcaa tatcgcttcg catattgccg atgccctggg gttgaccggc attccggccg
     4381 tacgtataga gactgcgtct tcggc
//
LOCUS       DASUTG010000417         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1399904, whole genome shotgun sequence.
ACCESSION   DASUTG010000417 DASUTG010000000
VERSION     DASUTG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399904"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /gene="ggt"
                     /locus_tag="VF089_09555"
     CDS             complement(<1..1566)
                     /gene="ggt"
                     /locus_tag="VF089_09555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6174248.1"
                     /translation="MKFTKAARPVVMGQNGVVSAGHPLGALSGVKILQEGGNAVDAAL
                     ASAFVMSVVKPEASGPGGDLFALVYMKKAGRVVALNSSGPAPAKATISYFQEHGLKSI
                     PINGPLSIAVPGAVDGWLELHKKYGTIELKRLVSDAVRIAREGFPISQEFAEAIEEHS
                     SESSWVDRFYRKSIGVSAPGKLLAQKGLAAIFEKIADHGRAGFYSGEIADQIVRTVRG
                     EGGILAQEDLQNIVAQWMEPLISTYRDTLVYEQPPVSQGFMVLEMLNIAEAWPFDSGS
                     MSRAEMIHLQVAAKKLAFEDRIRYLEDPSFGDPKIAMLVSKDYAAARRALAEDALKRS
                     PATGPNQASDTTYLCATDRDGNAISLIQSVFAPFGSRVIAGETGVVMNNRLCSFGLDP
                     AKANSLEPGKRPAHTLNTYMVFKGADVFAVGGSPGADEQPQTNFQIIHDLVDLKMDPQ
                     SAVEAPRWSHQPGTPPRDQLPEALRMEEGFDTQTLDDLRARGHKVSMVDRWSFGSAKI
                     IVRDHENGCWLGGA"
     gene            complement(1563..>2286)
                     /locus_tag="VF089_09560"
     CDS             complement(1563..>2286)
                     /locus_tag="VF089_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174249.1"
                     /translation="AAICRKHPTRFIGFFTLPTSSISASLDELERSVHELGLKGFGSF
                     SNLNGQALDREELFPIYERLAKYELPVYIHPTAPLASEATGIDIMPTLIFGWAFDSTV
                     AMTRLVYGRVLERFPEINFVVADVGGVLAFFAQRAKNIYSGRTEEIRQRYGLNENPLD
                     SFRQFYVDTADHPASTLKCVLDFFGPERLVLGTNYPYGPEEGCVLIKNSLTAIDGLNL
                     SSKDKEKVLGGNAAEILGLRAA"
BASE COUNT          497 a          663 c          607 g          519 t
ORIGIN      
        1 cgcgccgccc agccagcaac cattctcgtg atcacgaaca atgattttcg cgctgccgaa
       61 cgaccagcga tcgaccatag acactttgtg cccgcgcgct cgcaaatcgt ctaaagtttg
      121 cgtatcgaaa ccttcttcca ttcgcagcgc ctcggggagc tgatcccgcg gcggcgtgcc
      181 tggttggtgg ctccatcgcg gcgcttcgac ggcgctttgt ggatccatct tcagatcgac
      241 caagtcatgg attatctgga aattagtttg cggctgttcg tcagccccgg gactaccgcc
      301 caccgcgaag acatcggcgc ccttgaaaac catgtaggta ttgagggtat gcgctggccg
      361 tttccctggc tcgagtgagt ttgcttttgc cggatccagc ccgaagctgc acaaccgatt
      421 gttcatgacc acgccggtct ccccggcaat gacccgggag ccaaacggcg cgaacacgct
      481 ttgtatcaag gagatcgcat tgccatcgcg gtcggttgcg cagagatagg tcgtgtcgct
      541 agcttgattc ggccccgtcg ccgggcttcg cttcagagcg tcttctgcga gcgcacgacg
      601 agcggcggcg taatcttttg atacaagcat ggcgattttc ggatcaccaa aacttgggtc
      661 ttcgagatac cgaatgcgat cttcaaaagc gagtttcttc gccgcaacct ggaggtgaat
      721 catttccgcc cggctcatgc tgccgctatc aaagggccag gcttcggcaa tgttgagcat
      781 ctcgagaacc atgaagcctt gcgataccgg cggttgttca tacactagcg tgtcgcgata
      841 ggtggagatc agcggctcca tccactgtgc gacgatattc tgcaggtctt cttgcgccaa
      901 tataccgcct tcgcctctga cggttcggac gatttgatcg gcgatttcac cgctgtagaa
      961 tccagcgcgt ccgtgatcgg ctatcttttc aaatatcgcc gccaggcctt tctgagctaa
     1021 taattttccc ggcgcggaaa caccaataga cttccggtaa aagcgatcga cccaggaaga
     1081 ttcgctgcta tgctcctcga tggcttcggc aaactcctgg gatataggaa acccttcacg
     1141 ggcgatgcgc accgcatcgg atactaatct cttgagttct atcgtgccgt actttttgtg
     1201 aagctccagc cagccgtcca ccgcgcccgg gacggcaatg ctcaatggtc cattgattgg
     1261 aatgctcttc aatccgtgtt cctggaagta gcttatggtt gccttggccg gcgcgggacc
     1321 gctggaattt agcgccacaa ctcttccagc tttcttcatg taaaccaacg cgaacagatc
     1381 accgccaggc ccgctggcct cgggcttgac gactgacatg acgaaggcag acgccaaagc
     1441 ggcatcgacc gcgttgccgc cctcttgaag aattttcact cccgagaggg cgccgagcgg
     1501 atgccccgcg gagaccaccc cgttctgccc catgaccaca ggtcttgctg ccttggtaaa
     1561 tttcatgccg ctctcaaacc gagaatttcc gcggcattgc cgccaagaac tttctcttta
     1621 tctttgctgc ttaggtttaa accatcgatg gcagtcaaac tatttttaat gagcacgcaa
     1681 ccctcttccg gaccgtacgg ataattggtt ccaagcacga gccgctccgg accaaaaaaa
     1741 tccagaacac atttcaatgt cgaggccggg tggtccgcgg tgtccacata gaattgccga
     1801 aaggagtcca gggggttctc gttgagcccg taccgttgcc ggatctcttc agtgcgtccc
     1861 gagtaaatat tcttggcgcg ctgcgcgaag aacgccagaa cgccaccaac gtcggcgacg
     1921 acgaagttta tttccggaaa tctctcaagc acgcggccat aaacgagtcg ggtcatcgcc
     1981 accgtgctgt cgaaggccca accgaatatc aaagtgggca tgatgtcaat tccggtagct
     2041 tcactggcca acggcgcagt cggatggatg taaaccggca gctcatattt ggcgagccgt
     2101 tcgtagatcg gaaataattc ttcccgatcc aaagcctggc cgttaaggtt ggagaaagag
     2161 ccaaagcctt tgagccccaa ttcatgcacc gatcgctcaa gctcatcaag agatgctgaa
     2221 atggaactgg tcggcaaggt aaaaaagccg ataaagcggg ttgggtgttt ccggcatatg
     2281 gcggcg
//
LOCUS       DASUTG010000418         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_361678, whole genome shotgun sequence.
ACCESSION   DASUTG010000418 DASUTG010000000
VERSION     DASUTG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361678"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="moaA"
                     /locus_tag="VF089_09565"
     CDS             complement(<1..935)
                     /gene="moaA"
                     /locus_tag="VF089_09565"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX6174250.1"
                     /translation="MLVDALKRPIKDLRISVTDRCNFRCTYCMPLDEYEWINKKEILT
                     FEEISRLATLFIGLGVEKIRLTGGEPLVRQNLEKLVAKLSSIAGLQDLCLTTNGALLA
                     EKVAGLKASGLKRVNISVDSLNPEKFRQMTKRGDLTKVLEGIFAAKQHGLHPVKLNAV
                     IERGLNEDDILSLVEFSRDNGFALRFIEYMDVGNSNDWTSGKLVSKKEIIEKINARYP
                     LKEVGRAGGSAPSVDYEFIDGCGDVGVIASVTEPFCSSCTRVRITADGKIVACLFSGM
                     GHDVKGLLRGGASDEEISRFIVSIWEKRTDRYSAER"
     gene            1001..2560
                     /locus_tag="VF089_09570"
     CDS             1001..2560
                     /locus_tag="VF089_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174251.1"
                     /translation="MSDLLSCVRMSLRQQQIAVFLGTILIISGLFAAGVWLIAQRLTT
                     EATLQTALLMARQVEIALADSLKQRPVIVQTGHSRTQSSSFWSFLGNIFPGKSSPNKK
                     QIRVRRESRSEDVKGLMRAYVDRSGSIEAMWVLNAEGKVLYSSTGQENTQSDRVAQFH
                     EALRRGVTLINSRAQGKATYYDVMVPLQMPEGVRGPGGLRLWINPADWSELLSGMWRQ
                     LTLLFGLGGGIALLSAFLTTALYTRRFSIISETLRQAEAGTYAARPEYAREDEVGTSL
                     DLIDRLVMKQRNKVAAPAPAQRLALAARTLAHEVRTPLNALAIHLELLRSPVQCTEDS
                     DGAMDQPQRSIAVLDSSLRHVNQLIRDFTDYSAPVTMERKPVDVAEVLKMSLEAIGSA
                     CVEQKISLTQENFQGPWQVQGDATRLRQAFDNLLRNAMEAQPDGGEIRIMAEEDESRL
                     VVDINDAGPGVPPEQQPQLFEFGKTTKSSGSGIGLPLSQLIVEAHGGSLTYQGNGHGA
                     TFRLILPLEPS"
     gene            2563..3918
                     /locus_tag="VF089_09575"
     CDS             2563..3918
                     /locus_tag="VF089_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174252.1"
                     /translation="MARARSSVLVVDDDSATREGLTALLQSWGYTTVDAADGAAALKS
                     CDKELPHAIVTDLMMPAMSGLQFVECLGERVRQTAVIFVTGQAAVDTAVQAIKLGAYD
                     YLPKPLEPERLRITIEKALKQVALARETAALRRKLESPLGSYGALIGKSAPMRQLYRQ
                     ISQIAGTDAAVLITGESGTGKELVAQTLHDLSPRREATFFALNCAAISATLMESELFG
                     HEKGAFTNAIERHLGCFEQTDGGTLFLDEITEMPIEVQAKFLRVLEERQVRRIGGNAN
                     IPVSVRVVAATNREPATAVKEGKLRQDLFYRLSVFNLELPPLRQRGEDIPLLAQYFLE
                     VFAAKYHRPIPDWAGDYTAALQSHSWPGNVRELRNAMERLSVLSTGPTLSAKDFEQFC
                     LKPVVEEKSETAPISLAEAERQAIEKALAASQGNKTQAARLLGITPKTLSAKLALYGK
                     A"
BASE COUNT          967 a         1132 c         1116 g          927 t
ORIGIN      
        1 cgttcggccg aatagcggtc ggtccgcttc tcccagatcg agacaataaa tctgctaatc
       61 tcctcgtctg atgctccgcc gcgtaacagt cccttaacgt cgtgacccat gccagaaaat
      121 aggcaagcta caattttccc gtccgccgta attcgcacac gcgtgcagct cgagcagaac
      181 ggttcggtga ccgaagcaat cacgccgaca tccccacacc catcgataaa ctcatagtcc
      241 accgaaggag cgcttcctcc agcgcgtcca acttccttaa ggggatatcg ggcgtttatc
      301 ttctcaatga tctctttttt tgaaacgagt tttccggatg tccagtcgtt ggaattgccg
      361 acgtccatat attcgataaa acgcagggca aagccgttgt cacgtgagaa ctcgaccagg
      421 ctcaagatat catcttcgtt taaacctctt tcgatgaccg cgttcagctt taccggatga
      481 aggccatgct gcttagccgc aaagatgcct tctaggacct tggtcaagtc gccgcgctta
      541 gtcatctgtc tgaatttctc ggggttcagc gagtcgacgc tgatgttgac gcgcttgaga
      601 ccggatgctt tgagccctgc cactttttcg gcaagcaggg cgccgttggt ggtcaggcac
      661 aagtcttgta gtccggcgat ggaagaaagc ttggcgacaa gcttctcgag attctgccga
      721 accagaggtt cgccaccggt gaggcgaatc ttctcaaccc cgagtccgat aaatagggtg
      781 gccaaccgtg agatttcttc gaaagtgaga atctcttttt tattgatcca ttcgtattca
      841 tccaagggca tgcaataagt gcaacgaaag ttgcatcgat cagtcacgga tattctgaga
      901 tctttgatcg gccgttttag agcatccacc aacatagact taactatgca ggaaaggtgc
      961 tagaggcgca agttcggcca gcagttgaat tttgctggat gtgagcgatc tgttatcctg
     1021 cgtccgcatg agtttgcggc agcagcaaat agcggttttc ctgggtacca tacttattat
     1081 atcgggtctt ttcgccgccg gtgtctggct catcgcgcaa cggcttacta ccgaggccac
     1141 acttcaaacg gcactgttga tggcgcgcca ggtggaaatc gccttagcgg atagcctcaa
     1201 acagcgcccg gttatcgtcc aaaccgggca ctcgcgaacg caatcatcat cgttttggag
     1261 ttttttagga aatatatttc ccggtaaaag cagccccaac aagaaacaaa taagggtccg
     1321 cagggaatca cgcagtgagg acgtgaaggg tctcatgcgc gcttacgtgg atcgtagcgg
     1381 cagcatcgaa gcgatgtggg tcctgaatgc cgagggcaag gtcctttata gctcgacggg
     1441 acaggaaaac acgcaatctg atagggtcgc gcagttccat gaagcgttgc gccgtggggt
     1501 cacgctgatc aattcacgag ctcaaggcaa agcaacctat tacgatgtaa tggtaccgtt
     1561 gcagatgccg gagggggtcc gcggcccagg cgggttgcgt ttatggatca acccggcgga
     1621 ttggagtgag cttctgagcg ggatgtggcg acagctaact ctgctatttg ggttgggcgg
     1681 cggcatcgcc ctactcagcg catttcttac caccgcgctt tatacgcgcc ggtttagcat
     1741 tatcagcgaa acactgcgcc aagcggaagc gggaacttac gccgctcggc ccgagtatgc
     1801 cagagaagac gaagtcggca ctagcttgga tctgattgac cgtttggtaa tgaaacagcg
     1861 caacaaggtt gccgcacctg cacctgcgca acggctggcg ctcgcggcac gcactttggc
     1921 ccacgaggtc cgaacccctc tgaacgcttt ggcaatccat cttgagcttt tgcgcagtcc
     1981 ggttcaatgc accgaagact cagacggtgc aatggatcag ccgcagcgtt ccattgcagt
     2041 gcttgattca agcctgcgcc acgtgaatca gttgatacgc gatttcaccg attactccgc
     2101 tccggttacg atggaacgta aaccagtgga cgtcgcggaa gttctaaaga tgagcttgga
     2161 ggcgatcggg tcagcttgcg tagagcaaaa gatcagcctc acccaagaaa attttcaagg
     2221 tccttggcaa gtccaaggag atgcaactcg attgcgccaa gcctttgata acctcctgcg
     2281 gaacgccatg gaagcgcagc cggatggggg cgagatccgc atcatggcag aggaggatga
     2341 atctcgacta gtcgtggata tcaacgatgc cggtccggga gtcccacctg agcaacaacc
     2401 gcagcttttc gagtttggca aaacgacaaa aagttcgggc agtggtatcg gcttgccgct
     2461 cagccaattg attgtcgaag cacacggcgg atcgttgact tatcagggca acggtcatgg
     2521 cgctaccttt cgcttgatcc tgcccctgga gcctagttga taatggctcg ggctcgctct
     2581 agcgttcttg tagtcgatga tgactctgcg acccgtgaag ggttaacggc gctactgcag
     2641 agctggggct acactactgt cgacgccgcg gacggagcgg ccgcactgaa aagctgcgac
     2701 aaagaactac cgcacgccat tgtcaccgat ctcatgatgc ccgccatgag tggacttcaa
     2761 tttgtcgaat gcttgggaga acgggtgcgg caaacggcgg ttatctttgt taccggtcag
     2821 gcggcggtcg ataccgcagt gcaagccatc aagctcggtg cctatgacta tttacccaaa
     2881 ccgctcgagc ccgagcggct gcgaatcacg attgaaaagg ctctgaagca ggttgcgctt
     2941 gctcgcgaaa ctgctgcatt acgccgcaag ctcgagtcgc cgctgggttc ttacggggcg
     3001 ctcatcggca aatccgcgcc catgcgccag ctctaccggc aaatcagcca gattgccggc
     3061 actgatgcgg cggttttgat caccggcgag agcggcaccg gcaaagaact cgttgctcaa
     3121 accttacacg acctttcacc gaggcgagag gctacctttt tcgcgctcaa ctgcgcagcg
     3181 atctcggcaa cactgatgga aagcgagctt ttcggccacg agaagggcgc ctttaccaac
     3241 gccattgaac gacacctcgg ttgtttcgaa caaactgacg gcggtacact atttctcgat
     3301 gagattactg aaatgcccat cgaggtccag gcgaaatttc ttcgagtttt ggaagaacgg
     3361 caggtgcggc gtatcggcgg taatgcaaac atccctgtat cggtcagagt agtggcggcg
     3421 actaatcgcg agccggcaac cgcggttaag gagggcaaac tgcggcagga tctcttttat
     3481 cggctgagcg tgttcaatct cgagctgccg ccgctgcgcc agcgtggcga ggatatcccg
     3541 ctgctcgcac aatattttct ggaagtcttc gccgcaaagt accaccgccc gataccggac
     3601 tgggcagggg actacacagc tgcactgcaa tctcactcct ggcccgggaa cgtgcgagag
     3661 ttacgcaatg ctatggaacg tctctcggtt ttgtctacag gcccgaccct atcggccaaa
     3721 gattttgagc agttctgcct caagccggta gtcgaggaaa aatccgaaac ggcacccatc
     3781 tctctggcag aagccgaacg tcaagcgatt gagaaagcat tagctgcaag tcaaggtaac
     3841 aagacccaag ctgcgcgctt actcgggatc actccgaaaa ccttgagtgc gaaactcgcg
     3901 ctctacggca aagcataaca ttcttctcat acgtcgaacc agcactcgat ttctttgacg
     3961 ttctcgacca aagaaaaaat attcacccgc ggcgcgcgct aaagatctga cagaatagca
     4021 gcccatctat gttaatgatt cgactggcgg ttattatgaa ttcacgtttt aagacccagc
     4081 cttgcttgat cacgtatttc ttccctcacc atcacctttt taccgtcccg aaggccatgg
     4141 cc
//
LOCUS       DASUTG010000419         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1845607, whole genome shotgun sequence.
ACCESSION   DASUTG010000419 DASUTG010000000
VERSION     DASUTG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845607"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1195
                     /locus_tag="VF089_09580"
     CDS             101..1195
                     /locus_tag="VF089_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6174253.1"
                     /translation="MATVRGATLLGPDKLELREYPMPDIPADGGLVKVEMGGVCGTDV
                     KYLHGKLKVDYPIILGHEILGRVEKLGKNAAAIHHVKEGDRIILKGALGCGRCSDCRR
                     NNQRFCRQRTNYGGRTKCDQPPHLFGGFADYIYLAPDVLATKVSDALPAEAAALIGSV
                     MANGFQWAIRRGGVKMGDYVLIQGPGQQGLSCTFASRHAGAAKVFISGIGRDQPRLEL
                     AKKFGATRTINVEEEDVVDVIRKETNGEMCDVVVDVSGNPNAIVKSVDCLHRQATMVL
                     GGLTGDTTVTPMLMDKLVWNEIKLQGCFTADNDATEAALRLIEETKFPVQEMVSHTFP
                     LKDIEKCIRAVGGEIPEMFPTKALVNPALG"
     gene            1224..1991
                     /locus_tag="VF089_09585"
     CDS             1224..1991
                     /locus_tag="VF089_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174254.1"
                     /translation="MIEGLKDKVVIVTGGGHGIGKAYCLGFGQSGSRVVVADIDKPAA
                     DQVSAEIDQQGAQALALHTDVADERSTKDMAAAVLQGFGRIDVLINNAAMFATIPMNR
                     GRIDTIDPAEWDRLMAVNLRGLFFCCRAVLPSMRAQKSGKIINIASGTVFAGSPGRIH
                     YVTSKMATIGFTRTLAREVGDDNINVNVLAPGNTLSEENPSQEVIRLRESSAGLRALK
                     RIQMPQDVVGAMLFLASPLSDFITGQTVNVDGGISFV"
     gene            2201..3241
                     /locus_tag="VF089_09590"
     CDS             2201..3241
                     /locus_tag="VF089_09590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174255.1"
                     /translation="MNVNYPIIDADGHVMETDGELREFLEGRYRQGPHFKTYSYFPSL
                     DGWNRGFGVPGKVSETPAPKWLEFLDELGVHTTVLYPTGGLSLGLIQNPEWSCVIARA
                     YNSWIAERFSKQSQRLKAVALLPVHEPLEAAKELERAKGVGLVGGLLPAVTILNKGYG
                     HSDFDPIYQAAEKLDMPLTVHGAPSRGMGFDFFNKFLHVHTLEHPFAILIQFTHMVFE
                     GVFNRFPNLRVAFLEAGSGWLPYMMDRMDEEMEKPYRFQAPSLKQKPSEIIRSGQVWA
                     TCEVEEHALPQVIKQFNPACLMWPSDFPHERLPDMFSHDLPEFLGRTDISEENKKAIL
                     HDNPARFYRLEI"
     gene            3266..4024
                     /locus_tag="VF089_09595"
     CDS             3266..4024
                     /locus_tag="VF089_09595"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6174256.1"
                     /translation="MRLKDKVAIVTGGGVGIGKAYSHGLAKEGAKVVVADIQEAEAEK
                     VAADVKQSGGEAMAVPVDVTSFDKAQAMAQAAISAYGRIDILVNNAGLYSALKKKNFM
                     EIDSDEWDRVMAVNVKGLFHCVKAVYPAMKRQGKGKIINISSGTTLNGSPFFLHYVSS
                     KAGVIGFTRALAREVGGDNICVNSIMPGLTISGANQEGVMTAEQLADRRKRRSIQRDQ
                     YPQDLVGTVIFLASDDSDFMTGQSISVDGGMNMH"
     gene            complement(4082..>4315)
                     /locus_tag="VF089_09600"
     CDS             complement(4082..>4315)
                     /locus_tag="VF089_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX6174257.1"
                     /translation="WVEAEDYRLVERLAARDAPGFFHEIAKDQDKRRICGFSPLYSLI
                     HLLKNGNGQRLKYSQAFTPETASAVTFTSMVFD"
BASE COUNT         1002 a         1132 c         1251 g          930 t
ORIGIN      
        1 gccagcggtt tcatcgacaa gttgaccaag gaatatagag tcaaataaca acccatcggg
       61 agcgggttgg atcacagcac tgcaaattcg aggaggttct atggcgacag tacgaggcgc
      121 aacgcttctc ggtccggata agctggagct tcgcgagtat cccatgcccg acatccctgc
      181 cgatggcggg ttggtaaaag tggagatggg cggcgtctgc ggcaccgatg tgaagtatct
      241 gcatggtaag ctcaaagtcg attatccaat cattctcgga cacgagattc tcgggcgggt
      301 ggagaagctc ggcaagaacg ccgcggcgat tcaccacgtc aaagaaggcg accgcatcat
      361 tctaaagggg gcgctcggct gcggccgctg ttcggattgc cggcgcaaca accaacgctt
      421 ttgccgtcag cggaccaact acggcgggcg aaccaaatgc gatcagccgc cgcacctgtt
      481 cggcggcttt gcagattaca tttacctggc tcctgatgtt cttgcgacca aggtaagcga
      541 tgcgctaccc gccgaagcgg cggcgctcat cggctcggtg atggccaacg gctttcaatg
      601 ggcgatccgc cgcggcggtg tgaaaatggg cgactatgtc ttgatccaag ggcctggaca
      661 gcaagggctc tcctgcacat ttgcctctcg ccatgccggc gccgctaaag ttttcatcag
      721 cggcatcgga cgcgaccaac cgcggctcga gttggccaag aagttcggcg ccacccgtac
      781 catcaatgtc gaagaagaag acgtggtcga tgtcatccga aaggaaacca atggtgagat
      841 gtgcgatgtg gtcgtggatg tgtcgggcaa tccgaacgcg atcgtaaaat cggtcgattg
      901 tctgcatcgg caagctacca tggtactggg tggcttgaca ggtgacacca cggtaacacc
      961 catgctgatg gataaactgg tttggaatga gattaagctg cagggctgtt tcaccgcgga
     1021 caatgacgcc accgaggcgg cgcttcggct gatcgaggag actaagtttc cggtgcaaga
     1081 aatggttagc catacatttc cgctgaagga cattgaaaaa tgcattcgcg ccgtcggcgg
     1141 cgagattccg gaaatgttcc cgaccaaggc gctggtcaac cctgcgttgg ggtgatcaag
     1201 gaaaggcaac cctaagtcat acgatgatcg aaggactcaa ggacaaagtc gtcatcgtca
     1261 ccggcggcgg ccacggcatc ggcaaagcat actgcctcgg cttcggccag tcgggctcgc
     1321 gcgtggtcgt ggccgacatc gataagccgg ccgccgatca agtcagcgca gaaatcgacc
     1381 agcaaggcgc acaggcgctg gcgcttcaca cggatgttgc cgacgagaga tcaaccaagg
     1441 acatggccgc cgcggtgttg cagggtttcg gcagaatcga tgttctcatt aacaacgctg
     1501 cgatgttcgc gactatcccc atgaaccggg gacgcataga caccatcgac cccgccgagt
     1561 gggatcggct catggcggtg aacctaaggg gtttgttttt ctgctgccgc gccgtgctac
     1621 cgagcatgcg ggcgcagaag tccggcaaga tcatcaatat cgcctcggga actgtgttcg
     1681 cgggaagtcc cggaagaatt cactacgtga cgtcgaaaat ggcaaccatc ggttttaccc
     1741 ggaccttggc tcgcgaggtc ggcgatgaca atatcaatgt taatgttttg gcgccgggta
     1801 ataccctctc tgaagaaaac cccagccaag aggtcattcg tttacgcgaa tcctctgccg
     1861 ggttgcgcgc attgaagcgg attcagatgc cgcaggatgt ggtcggcgct atgttgtttt
     1921 tggcctcgcc gctcagtgac ttcatcaccg gccagacggt gaatgtcgac ggcggcattt
     1981 cctttgtcta agtccaatta acgagcttat ttgaaggacg aagcacacga ggaactgatg
     2041 aagcgacttc ctagtttaaa gtcgcaaatt gatgcttctt cgtactactt ttgtcgaaag
     2101 ttttcgcgct tgcggaatcc tctgcgcctg ggcggaagtt gctttgctaa ccagcgcgcg
     2161 cagcacgcgt tggggtaatg agttggcagg agattagcct atgaacgtga actatccgat
     2221 catcgatgct gatggccatg tgatggaaac agacggcgag ctgcgcgagt ttcttgaagg
     2281 ccgttatcga caaggtccac actttaaaac ctatagttat tttccttcgc ttgatggctg
     2341 gaaccgcggt ttcggcgttc cgggcaaagt ttcggagaca cccgcgccta aatggctcga
     2401 atttctcgac gagcttgggg tacacaccac cgtgctttac cccactggcg gcttgtcgct
     2461 cggattgatt cagaatcctg agtggtcctg cgtgatcgcg cgcgcttaca attcatggat
     2521 agccgagcgc ttttcaaagc aaagtcaacg gctcaaagcg gtcgcgctcc tgccggtgca
     2581 cgagccgctc gaagcggcaa aggagctaga acgagccaaa ggggtcggac tcgtcggcgg
     2641 gcttctgccc gctgtcacga tcctgaacaa aggctacggt catagcgatt tcgatccgat
     2701 ctaccaggcc gctgaaaagc tcgatatgcc gctgacggtc catggcgcgc ctagccgcgg
     2761 catgggcttt gacttcttca acaagtttct ccacgtccat acgctggagc atccgttcgc
     2821 gatcttgatc cagtttacgc acatggtgtt cgagggtgtg ttcaaccgct ttccgaatct
     2881 gagagtcgca ttcctcgaag ccggttcagg ctggttaccc tacatgatgg accgcatgga
     2941 tgaagagatg gagaagccgt atcggtttca ggcgccttcg ttgaagcaga agcccagtga
     3001 gatcattcgg tcgggccagg tttgggcgac ttgtgaagtt gaagaacatg cgttgccgca
     3061 ggtcatcaag caattcaatc ctgcttgcct gatgtggccg tcggattttc cccatgagcg
     3121 tctgcctgat atgtttagtc acgacctgcc ggagttctta ggacgcacgg acatcagcga
     3181 ggaaaacaaa aaggcaatcc tgcacgacaa cccggcacga ttttaccggc tggagatctg
     3241 atcagctcga aatggaggaa ctcacatgcg cctaaaagat aaagttgcga tcgtcaccgg
     3301 cggcggcgtc ggcatcggca aagcatactc tcacggcttg gccaaagaag gcgctaaggt
     3361 tgtggtcgcc gatattcaag aagctgaggc agaaaaagtt gccgccgacg tcaaacagtc
     3421 cggcggtgaa gccatggcag tgccggttga tgtgacttca ttcgacaaag ctcaagcgat
     3481 ggcccaagcc gcgattagcg cctatgggcg gatcgacatc ttagtcaata acgctggact
     3541 gtattcagca ctgaaaaaga aaaatttcat ggaaattgac agcgacgaat gggaccgggt
     3601 catggccgtg aacgtgaaag gcctgtttca ctgcgtcaaa gctgtttatc cagctatgaa
     3661 acgacagggc aaagggaaga taatcaatat ctcatccgga acaacgttga acggctcgcc
     3721 attttttctt cattacgtct cgtccaaggc tggcgtgatt ggcttcaccc gggcgctggc
     3781 acgggaggtt ggcggcgata acatctgcgt taattcaatc atgccgggtc tgaccatttc
     3841 cggcgcgaat caggaaggcg tcatgaccgc cgagcaattg gcagaccgaa gaaagcgtcg
     3901 ctcgattcag cgcgatcagt atcctcagga tctggtgggc acggtgatct ttctcgcgtc
     3961 cgatgacagc gacttcatga ccggccagtc gattagcgtc gatggcggca tgaatatgca
     4021 ttgaacgcgt aagacaggcc agagagacca tcaagacaaa agcaaactca agcgtttcca
     4081 ttcagtcaaa gaccatgctg gtaaaggtga ccgccgaggc tgtttcggga gtaaaggctt
     4141 ggctatattt taatcgttgt ccgttgccgt tcttgagcag gtgaatcagc gagtaaagcg
     4201 gtgagaaccc gcagatccgt ctcttgtctt gatcttttgc gatctcgtga aaaaatcccg
     4261 gggcatcgcg ggcggctaga cgctccacca gacgataatc ctcggcctct accca
//
LOCUS       DASUTG010000420         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2141675, whole genome shotgun sequence.
ACCESSION   DASUTG010000420 DASUTG010000000
VERSION     DASUTG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141675"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VF089_09605"
     CDS             complement(<1..681)
                     /locus_tag="VF089_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174258.1"
                     /translation="MSTVQATLWLYTTALRRSWESLLKNWVVSFAPWVYAVLLSTLAL
                     VVAPLGIIGGLLLGLATQACISSGLYLIKNIVESGRADLKDFLHGFTVYLWDLLGIAF
                     ILWIPMRVLAMALASTPNGGLIFALIQLVIYILLNPVPELIYQSRSSGLGLISDSYNF
                     IVENWLEWLLPNVVMTIAGYLFLNILSTITTGLPSVVQIFIVAVAIGLCLSYFMIFRG
                     FLFAELHGS"
     gene            2225..2821
                     /locus_tag="VF089_09610"
     CDS             2225..2821
                     /locus_tag="VF089_09610"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174259.1"
                     /translation="MFTRIKLTFFVSLVALFCVALPLQAGAPTEEIRTAIDKGVQILK
                     SAKLDSSKERAPVIAQLREVVYPRFDFEEMAKRSLGSHWRRLGPQQQKEFVTAFTDLL
                     ETTYADKIDLYEGQQVEFVGETIDKNYAEVNTRVVGKNGESYSVDYRLHQVGGKWRIY
                     DVIAENISLVNNYRSQFNRVVVNSSVEELIKRIKQKSN"
     gene            3826..4500
                     /locus_tag="VF089_09615"
     CDS             3826..4500
                     /locus_tag="VF089_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174260.1"
                     /translation="MTPTAVWRNTMHSGEAPIRDSFTNARPELAWLPYPNFNQDNLHG
                     TIDETSPEGEPGVGVLDNKNVGGFAALSYAATNSLSDFYLKTWIYAQVTPGEKGPLNG
                     IAFRIDTVSGNFYRVATQFMGPDPALSLAYVGKETNHYPKYLARWKGAEIPAGAPQSS
                     GWQKIAISVQDGKAEIYWNETKLPDGPFPIDRVQSGYIGVYANFVGGLGHAETKVDGL
                     RVWPRK"
     gene            complement(4526..5020)
                     /locus_tag="VF089_09620"
     CDS             complement(4526..5020)
                     /locus_tag="VF089_09620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6174261.1"
                     /translation="MIIRVARIEDAAVLTKAEQDTAQIPGLLVSRPHELTTESFAAKI
                     TELATLGRYIVAENNERIIGHALLDPMRLEATAHIFRLTIVVHSGFQNRGVGEALMRD
                     LMNWAKQAPNLRKIELLVRATNERAIRLYFRMGFIEEGRFRERVGLVNGGFVDDLAMA
                     WFPR"
     gene            complement(5073..>6029)
                     /locus_tag="VF089_09625"
     CDS             complement(5073..>6029)
                     /locus_tag="VF089_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174262.1"
                     /translation="QPAFAQDSQLIAGAKKEGGKVLVYSSMETSIAEKVGNAFKKKTG
                     IEMEIWRASSTKVMDRALNEHRAGRPLADVIVTIADPMLIMQRAGVFAKYDSPAAADY
                     PKELIDPNLGPSYRNLLIGIVYNKNVIKPGDAPMSLEDLAKPQYKGKLVMPDPTQHTT
                     TTQWLASLHKLMGKEKAEKYIRDLAATKPTLVESLLPPVERAATGEIPIAITLVHYTY
                     VFGQTGAPLDYVRLPKMLGDGNYIALNNKAPHPNAGKSFIDYFLDDESMKIMANMGEF
                     VNRKGIFPPLPGADKIQFVPMDKFDTKTYAEKKKEFQRIFLR"
BASE COUNT         1502 a         1568 c         1511 g         1448 t
ORIGIN      
        1 actgccgtga agttcggcaa ataaaaaccc tcgaaagatc atgaaatagc tgagacagag
       61 accgatggcg actgcgacaa tgaagatttg caccacgctc ggtagtccgg tggtgattgt
      121 gcttaaaata ttaaggaaca aatatccggc tattgtcata actacgttcg gcaaaagcca
      181 ctccagccaa ttctcgacga taaaattata actatcgcta atgagcccaa gtccggaaga
      241 acggctctgg tagataagct caggaaccgg attcagtaga atataaatta caagttgaat
      301 caacgcgaag atcaggccgc cattcggcgt cgatgcaagg gccatcgcca aaactcgcat
      361 cggaatccat aatataaagg cgattccaag taaatcccag agataaacgg taaagccatg
      421 gaggaaatcc ttgagatcgg cgcgaccgct ctcgacgatg ttcttgatca agtatagccc
      481 ggagctaata cacgcctgag tcgccaagcc caaaagtaaa ccgccgataa tacccaacgg
      541 cgcaactacc aacgcgagag tggatagcag gaccgcatag acccacggag caaaactcac
      601 aacccaattc ttgagcagac tttcccagga gcggcgcaac gccgtcgtgt aaagccatag
      661 agtcgcttgt accgtactca ttgacattct tataacagaa aaaagttctg ccgtgcagca
      721 aacggtctca cgacatgatc tgcctgtctt gattcacctc gtttgttttt tattctaagc
      781 cggagctaac ctagtgagtc ttggcaccga gaatcgaaga atgaaaaaga tttactgcct
      841 catcttacgg acctgtagtc atttcctagc aaccagagca gcctgatcct acagccgtac
      901 agaattgcac tctgacagta gccgccaaat acaggatatt cccgctttcc tgatccgcta
      961 agtgtttatc ccatttgccg tacttcagca ttttcagcgg tttagccagt gatcggccga
     1021 tctgatcaat gatggctaat tttttgcacg cgagcttcga tcactaccac agctgtttcg
     1081 ttggactggc agaggagtcc ggagttggat gagactggaa gcttgtcctt cagacactct
     1141 cgacttgtct tcagtaccaa tggaagctgc gacgtatgct gcgcattctt caatccggtg
     1201 cattgatagc gataaagcga caaccttctc aagatactgg tgttgaaaac tttaaaggcg
     1261 acaatgctat tttctctgag aacctctggc caacatacga atgtttctgg ttgatccgat
     1321 aaactctgtt catccgttcg caagatcagt tcgctacagc aaatgattta tatagtcact
     1381 tacgacgaac tgcgatatca cagctctaac gcgaagcggc tgatcttagt cgaatcgaat
     1441 gtggctttgg aactccaacg tgtcacccgc ctagtgaaaa tgctaacgga cggcaagttc
     1501 gcggcggcga gcgtggagat cgccggacag agagactggg acacgaaaca tgtgagacat
     1561 cccgccatcc gtacttacca gctcgattag ctttagagga gcgcccgccg actagttgga
     1621 tatcgatccg atgatgaagg cgtcttgatt cctcgacgca aacattttac tccagagcga
     1681 gaacaggaac ttaaaacgac cagttcaaaa gaatcaccgt cctcccgtct cccaaactag
     1741 caggttgctg aaaaaagcct cttgcaggct gctcaaaaag atccagatgc gaggcgattg
     1801 agcggcaacg aagcatatgg gtttttttca gcaagcctgc tagggcatag ccgcggaagc
     1861 acaacataaa gggcgcggaa gacacaccga ttctgtggcg caacgagatt ccggccgcgg
     1921 ttgttctctt caaagctact ccatcgaagc tcggacctta agacgaactc tctttgcgca
     1981 cgacgttgat cggagcggcg gtatattcat ttgcgggatt tagtgctatt ttcaaatcac
     2041 atcaccggat gacattctga gatgggcgcg gtggttttgc tttatgacga ggctcgccct
     2101 aagtttcccc aaagtctcgc ggtgaaactc accgatgttc cgctcttctc cctgtcgttt
     2161 cgcgtaatct agatgtacta gctccctgtg gtacgtagta aaactggaaa gcgatcagca
     2221 caatatgttt acacgcatca aactaacctt ttttgtttca ttagtagcgc tattttgcgt
     2281 agctctgcca ctgcaggctg gagcccccac cgaggaaata cgtacagcta tcgacaaagg
     2341 cgttcaaatc ttgaagtccg ccaaactcga cagtagcaag gagcgcgctc ccgtcatcgc
     2401 tcaactgcgc gaggtcgttt acccccgatt cgatttcgaa gaaatggcca agcgatctct
     2461 aggctctcac tggcggcggc tcggtcctca acagcaaaaa gaattcgtga ctgcattcac
     2521 ggaccttctg gaaacgacct atgcggacaa gatcgacttg tacgaggggc agcaggtcga
     2581 gtttgtcggt gagaccatcg acaagaacta tgcggaagtg aacacacgag tggtcggcaa
     2641 gaacggcgag agctattcgg ttgactaccg gcttcaccaa gtcggcggca agtggaggat
     2701 atacgacgtg atcgcggaga acatcagcct ggtaaataac tatcggtcgc aattcaatcg
     2761 tgtcgtggtt aactcatcag ttgaagagct cataaagaga atcaaacaaa aatccaatta
     2821 gccgagcgcc tctgacgaag ttcacaggtg tccaaaattg ccggacgcaa cctcatcgct
     2881 ctccgggtgc cgctgtttct tccggaactc agaacatcct gctccaactt tgaattccta
     2941 tcgggtggtc agtcaactgg cagctaagcg ttaatgtaat ctacagcgct tagctccgat
     3001 gatcgatcat gctcttctgc atagttaccg gagtacgtcg gagtaccggt aattcaaaga
     3061 caagctccag ttaggtcggt tgccaaaaag gatggcgagt ttgcgaagat gggggaagac
     3121 tggccaaaac agtgagactg atcgtgtgga taactccccc cgaccggctt aggggattat
     3181 tgtccaatca tgccaaagat tgccaaaaat ggtagaaatg gcgccttcat gaggatgttt
     3241 cggcgactcc ttactactaa taagagctcg gatttgcgag gttttttttg cccaatcgga
     3301 aggcaattcg ctcagcggca gtactgcacg acgggccgct gccagcaagc atcggttatc
     3361 cacaaagtta tccactgctt ctgtgaacat ccgattctta ccactcgttc tatccggtcc
     3421 atgacgatgg aaagctcggc gccatcgatg atgccacgag ttgatatgcc gccaatgcag
     3481 gcctatcggc caccgccctg tgagttcgta gtgcaaaaca cggtgcgtat gcgagctcgc
     3541 tcgcggaggt tcgaaccgta agagaatgtc cgtaaccgag atccatcagc cgacatgcag
     3601 cgcacggcaa ttttcaccgg ccgccatgca gcaggagaac actcttcacg ttcaacgctg
     3661 tcaaagtatc cgagttgtca aatttctcta gaccagctaa tcgtgcagta gtcattttga
     3721 ttgttcaacg cattcgccgt gcttttaaaa tattcattaa ccggtccttg gcaggttcgg
     3781 agcatcatct ggatcgtgct accaatgctt ttaagctgca gtccgttgac ccctactgcg
     3841 gtatggcgca atacgatgca ctccggtgaa gcgccgatca gggacagttt cacgaacgct
     3901 cggcctgaac tcgcctggtt gccctatcca aatttcaatc aggacaatct gcacgggacg
     3961 atcgatgaaa cgtcacccga aggcgagcct ggggtcggcg tgttggataa caaaaatgtc
     4021 ggcggcttcg ctgcactctc ttatgccgcg acaaattctt tatccgattt ctacctgaaa
     4081 acctggatct acgcgcaggt cactcctggc gagaaggggc ctctaaacgg catcgctttt
     4141 cgtatcgata ccgtttccgg caacttctac cgagtggcta ctcagttcat gggaccggac
     4201 ccggcgctgt cgctggcgta tgtcggaaag gaaaccaatc actatcccaa atatctcgcc
     4261 cgctggaagg gagccgagat cccagccggc gccccgcaga gcagcggctg gcagaaaatc
     4321 gctatttcag tccaggacgg aaaagccgag atctactgga atgaaacgaa actgccggac
     4381 gggccttttc ccatagaccg ggtgcaatcg ggttacatcg gcgtctatgc aaacttcgtc
     4441 ggcggtctcg ggcatgccga aacaaaagta gatgggctgc gtgtgtggcc gcggaagtaa
     4501 ctagttcgca gaattagatc gttggttatc gtggaaacca cgccatggct aggtcatcga
     4561 caaagccgcc gtttaccaat cccactcgtt ctctgaagcg cccctcttcg ataaatccca
     4621 tcctgaaata gagacggatc gctcgttcat ttgtcgctct tacaagtaat tctatttttc
     4681 gtagattggg cgcctgcttc gcccagttca tcagatctct catcagtgcc tctccaacac
     4741 cccgattctg aaatcccgaa tggaccacga tagttagcct gaagatatgt gcagtcgctt
     4801 ccagccgcat gggatccaac aacgcgtggc cgatgatcct ttcattattt tccgccacga
     4861 tatagcgacc cagtgtagcg agctcggtaa ttttcgcagc gaatgattcg gtagtgagtt
     4921 cgtgcggacg cgacacgaga agccccggaa tttgtgcggt gtcctgctct gccttcgtaa
     4981 gaaccgcggc atcttcgatt cgcgccacac ggataatcat acagggctgc ctgcttccac
     5041 cagccagatt aggacatgtg tccgcttcct gtttaccgaa gaaaaattct ttggaactcc
     5101 ttcttctttt cggcgtacgt cttcgtgtcg aacttgtcca tgggaacgaa ttgaattttg
     5161 tcggcgccgg gtagcggtgg aaagatgcct ttacgattaa cgaactcgcc catattggcc
     5221 atgattttca tgctttcgtc atccagaaaa tagtcgatga acgattttcc tgcgttggga
     5281 tgaggcgcct tgttgttgag cgcaatgtaa ttgccgtcgc cgagcatttt cggcagcctt
     5341 acatagtcta agggagcgcc ggtctggccg aagacatagg tgtaatggac cagggtaatc
     5401 gcgatcggaa tctcgccggt ggcggcacgc tcgaccggag gcaggagaga ttccacaagc
     5461 gtgggtttcg tcgccgccag atcgcgaata tacttttcgg ctttctcttt gcccatgagc
     5521 ttgtgcagac tggcaagcca ctgcgtcgtg gttgtgtgtt gggtgggatc gggcatgacc
     5581 aatttaccct tgtactgggg cttggcgaga tcctctaacg acattggagc atctccgggt
     5641 ttgattacgt tcttgttgta gacgatgccg ataagtagat tgcggtagct cggacccaag
     5701 ttcggatcga tcaactcttt aggataatcc gccgcggcgg gcgagtcata tttggcaaag
     5761 acccccgcgc gttgcatgat cagcatcgga tcggcaatgg tgacaatgac gtcggctaat
     5821 ggccgaccgg cgcgatgctc gtttagcgct cggtccatga ctttagttga agacgcgcgc
     5881 caaatttcca tttcgattcc ggtctttttc ttaaaagcgt ttcccacttt ctcggcgata
     5941 ctcgtctcca tagagctgta cacaaggacc ttaccgccct cttttttcgc tcccgctatg
     6001 agctgggaat cttgagcgaa ggccggttg
//
LOCUS       DASUTG010000421         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1102623, whole genome shotgun sequence.
ACCESSION   DASUTG010000421 DASUTG010000000
VERSION     DASUTG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102623"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF089_09630"
     CDS             complement(<1..187)
                     /locus_tag="VF089_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6174263.1"
                     /translation="MDARGAVVSLHIAPAGAARVISVGEIQALAGKGLEGDRYYLKTG
                     TFSKTPGSGREVTLIEIE"
     gene            complement(190..1050)
                     /locus_tag="VF089_09635"
     CDS             complement(190..1050)
                     /locus_tag="VF089_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6174264.1"
                     /translation="MEPIPASILTTANEKSRRLRELVRAPEILIMPGAYDVLSALLFE
                     QMGFKAIQGTSGGIAAAVGYPDGEAMSRDQFIQLSGNFAAAVSVPFNADGERGYGDEN
                     GVRDTVRALVARGVAGMNLEDGAAKGKGGLVEVSEQLRKIKAVMDTKRELGSEFFLNA
                     RVDSFHVITDNSKKAVDEAIERGNAYAEAGADCIFYLFLHSAEIIGRLAKEVKAPISI
                     LAGPQSPGVQELQDLGVARVSYGSGFTKAAITATRRLAQEILEKGTCNLLKEGMQTPD
                     INALLARKQK"
     gene            1434..1910
                     /locus_tag="VF089_09640"
     CDS             1434..1910
                     /locus_tag="VF089_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174265.1"
                     /translation="MSKIRGISHVVLYVNDLEKMVAFYRDVLGLVEYRENPGRMVFLT
                     PNPDIDDHQIALAKGREGDAKIIGHIAWSVDTPADVKAFYEKFKALGVPIDHCVSHAY
                     QEMGNTVSCYFLDPEGNRLEIYALLPERDHARINRPLDLDKSVDEIVAQASGLGAV"
     gene            2102..2422
                     /locus_tag="VF089_09645"
     CDS             2102..2422
                     /locus_tag="VF089_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX6174266.1"
                     /translation="MKQTCFVCQNVDCLSRGSEELMKELTNEVAAKSIDAEVKSYICF
                     GGCDIGPNIVVHPQKVWYAGVKKEDVPEIVAALASGGTVSRLDSADPALKEIVFSLLD
                     AGVY"
     gene            complement(2571..>3244)
                     /locus_tag="VF089_09650"
     CDS             complement(2571..>3244)
                     /locus_tag="VF089_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6174267.1"
                     /translation="NVRIGVVSFAGTASIVQMPTQNREDIIAAIDRFQLQRATAIGSG
                     IIVSLAAIFPDAGIDVSSFIYGRDGPRGVPLDQSRKTDKPAPKPVPPGSYTSAAIILL
                     TDGQRTTGPSSIEAAQMAADRGVRVFTVGIGTTSGETLGFEGWSMRVRLDEASLKAIA
                     NITRGEYFYAGTANDLKKVYQSLNSRLVLEKKETEITALFSAAAAVAALISGLLSLLW
                     FNRIL"
BASE COUNT          714 a          888 c          892 g          750 t
ORIGIN      
        1 cttcgatttc gatcaaggtc acttcgcggc cgctcccggg agttttgctg aaggtgccgg
       61 ttttaagata atagcgatcc ccttccagcc ccttcccggc gagggcctgg atttctccga
      121 cggaaatgac ccgtgcggcg ccggccggag cgatatgaag ggacacaacg gcgcctctcg
      181 cgtccatgct cacttctgtt tccgtgctag tagcgcgttg atatcgggtg tctgcattcc
      241 ttccttcagc agattgcatg tgcccttttc gagaatctct tgcgccaatc gtcgggtggc
      301 ggtgatcgca gccttggtga aacccgatcc atagctgacc cgcgcgacac ccagatcttg
      361 cagctcttga acgcccggcg actgcggccc ggcgagaatg ctgatgggcg ctttaacctc
      421 cttggctagc cgcccgataa tctcggctga atgaaggaac aggtaaaaaa tgcaatcggc
      481 gcctgcttcg gcgtaggcat tgcctcgttc aatcgcttca tcaaccgctt tctttgaatt
      541 gtcggtgatc acgtgaaagg aatcgacacg cgcattgaga aaaaactcgc tgcccagctc
      601 gcgtttggta tccatgaccg ctttgatctt gcgtagttgt tccgagactt cgaccagccc
      661 gcctttgcct ttcgccgcgc cgtcttcgag attcataccg gcgacaccgc gcgccaccag
      721 agcgcgcacc gtatcgcgaa cgccgttctc atcgccgtag cctctctcac cgtcggcatt
      781 aaacggaacg cttaccgccg cggcaaagtt gccgctgagt tggataaact gatcgcgcga
      841 catcgcttca ccatccggat aaccgaccgc tgcggcaatg ccgccgctgg ttccctgaat
      901 cgccttgaag cccatctgct caaacaacag ggcgctgaga acgtcatagg ccccgggcat
      961 gataagaatc tccggcgcgc gcaccagttc cctgagcctg cggctcttct cgttggctgt
     1021 ggtcaatatc gaagctggaa tcggttccat gaatctctct ccttcttatg gattttacag
     1081 ttcaaatagt tcaagtcgtg aaagcagttc aagcggtcga acatgacaga ccttgaacca
     1141 ttgaaccatt gaaccgttga acgacttcgt tgtgaactcg catcctaagt cggaacaaca
     1201 cactctagca agaggcagag atcgagagcc ggtcgcttgc agaattattc gatctagcgc
     1261 gagttcggcg cgcattttgc ggcgagctac tgctcttgac gctcttgttt cctccctaaa
     1321 cacaaacatg gtttacgata cgagtttgtt tcgagcgttg acatacgatg aagctatcgg
     1381 gtaggttttc accctaggat cgcttgactc tatcaacata aaggaggctt ccgatgtcga
     1441 agatcagagg gataagccac gttgtgcttt acgtcaatga cttggaaaag atggtagcgt
     1501 tttaccgcga cgtgctcggg ctggtcgagt atcgggagaa ccccggtcga atggttttcc
     1561 taaccccgaa tccggacatc gacgaccacc aaatcgcact ggcgaaggga cgagaaggcg
     1621 acgccaagat catcgggcac atcgcctgga gcgtcgacac gccggccgac gtcaaggcat
     1681 tctatgaaaa gttcaaagct ctcggagtgc ccatcgatca ctgtgtcagt catgcatacc
     1741 aggaaatggg aaataccgtc tcctgttatt tccttgatcc ggaaggcaac cgtctggaaa
     1801 tctatgctct cttgccggag cgcgaccatg cgcgcatcaa tcggcctctc gacttagaca
     1861 aaagtgtgga tgagattgtc gcacaagcca gtgggctcgg tgcggtctaa ggatcagcat
     1921 tgctgttaaa attcggaaac ctttgttatg caatcggctc tcaatccgat gttgaatagc
     1981 gagctgggtg agcggttgaa cctttgaacg ttgaacaaat tcgtcgagcc cttgaacgag
     2041 gctgatcttg gatccagagg ttgtttaaga taactacaga tcaggaagga acttgagctc
     2101 aatgaaacag acttgctttg tctgccaaaa cgttgattgc ctgagccggg gctcggaaga
     2161 gctcatgaag gagctcacca acgaggtggc ggcgaaatcg attgatgccg aggtgaagtc
     2221 gtacatctgc tttggcggct gtgatatcgg tccgaacatt gtcgttcatc cgcaaaaagt
     2281 ctggtacgcc ggcgtaaaaa aggaagacgt gccggagata gtcgccgcgt tggccagcgg
     2341 cggcacggtc agccggctcg atagcgcaga tcctgcgttg aaggagattg tattttcgct
     2401 tttggatgcg ggagtgtatt aattacttac cagccgtcga atatagtagg ttctgttctc
     2461 ttccccagtt gacagccgga ggattcgtcc cggcgccttt agcggtttgt ctcaaggcag
     2521 tggaatctag acaaaacaaa tccatagcct ttcagacgct gggtgagcca ttacaggatt
     2581 cggttgaacc acagcagcga aagcaaaccg gaaatcagcg cggcgaccgc tgccgctgcc
     2641 gaaaatagcg cggtgatctc ggtctctttc ttctccagaa ccaaccgtga gttgagcgat
     2701 tggtagacct tctttagatc atttgccgtg ccggcgtaaa aatactcgcc gcgggttatg
     2761 ttcgcgatcg ctttgagcga ggcctcgtct aggcgcaccc tcatggacca gccttcaaag
     2821 cccagcgtct cgccacttgt ggttcctatt cccaccgtga agactcgaac gccgcggtcg
     2881 gcggccatct gagccgcctc gatagaactc ggacccgtcg tcctttggcc gtcggtgagc
     2941 aaaatgatcg cggcggaagt gtaagatccc ggtggcaccg gcttcggcgc cggcttatcc
     3001 gttttgcgcg attgatcgag cgggacgccg cgcgggccgt cgcgaccgta aatgaatgag
     3061 ctcacatcga tgccggcatc cgggaagatc gccgccaacg atacgatgat gccgctgccg
     3121 atggcagtag cgcgttgaag ttggaaccga tcgattgcgg caatgatgtc ttcgcgattt
     3181 tgtgtcggca tctgcacgat ggatgctgtt ccagcaaagg acacgacgcc gatgcgcacg
     3241 ttgg
//
LOCUS       DASUTG010000422         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_806462, whole genome shotgun sequence.
ACCESSION   DASUTG010000422 DASUTG010000000
VERSION     DASUTG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806462"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VF089_09655"
     CDS             <1..913
                     /locus_tag="VF089_09655"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX6174268.1"
                     /translation="ETAGRLSNSVKLNLPFKTPYDWQAIIQYFQSHSIPGVERVTGDS
                     FARVFRVGNEVGFVEVRPLLAKSQLKVWVVSEDPETTSETARRVRKMFDLDCDPVLIA
                     KSFERIPVLADLCRRFPGLRVARGWDCFETAICTILGQLVSAGHRERLIGQLVSNYGE
                     EVVDPSTSKRAYLFPAAGVLASSDLSAIGTTTARREAIRDFSRRVLSRAISLAEDQDP
                     AAFREALLETKGLGPWSADYIRLRTGDNDAFPKTDLILKRVLDLHPDLDLERIKPWRS
                     YAAVYLWKEFAHTLSRSRNRKRHDAIL"
     gene            900..1412
                     /locus_tag="VF089_09660"
     CDS             900..1412
                     /locus_tag="VF089_09660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6174269.1"
                     /translation="MLSYKVMESPVGKLKLVANANALVAILWERERPNRIKLAILKSD
                     PQQPILLEAERQLKEYFAGTRNEFNLPLEPAGTEFQKKVWQALREIPFGQTRSYLDLA
                     KSIGSAKAVRAVGAANGKNPLSIVVPCHRVLGGDGALTGFAGGLEVKARLLAHEGCGR
                     FSARGEEKLT"
     gene            1409..2125
                     /locus_tag="VF089_09665"
     CDS             1409..2125
                     /locus_tag="VF089_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX6174270.1"
                     /translation="MSSPATIREQTIDWERACAALDERGFALLPRVLVRKTCQDIARY
                     YNDDHRFRSRIEMSRYSFGRGEYKYFGYPLPAIVQQLRSSIYPHLAPLANRWADRLGS
                     KLPRYPETLADFLKQCHRVGQNRPTPLLLKYGPGDFNCLHQDLYGDSVFPFQLTFFLS
                     QRQSDFGGGEFVLAEQQPRRQSRVEVLTPDQGDAVIFSVQHRPVRGARGYYRANLRHG
                     VSTIHFGERYTLGIIFHDAQ"
     gene            complement(2333..2929)
                     /locus_tag="VF089_09670"
     CDS             complement(2333..2929)
                     /locus_tag="VF089_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX6174271.1"
                     /translation="MNNPERRICVFTGSRDGSHPGYAEAARQLGRELVVRNYGLVYGG
                     GNVGLMKVIADTVLELGGYVTGVIPDSLVSREVAHLGLSDLRIVESMHERKAMMAELS
                     DGFIAMPGGIGTMEEFFEVLSWAQLGIHRKPCGLLNTGGYYDPLIEFLDHAVEHDFLK
                     QKHRSLLILAREPPEILSRFEAFITISGDDQHFDSSKI"
     gene            complement(2995..3243)
                     /locus_tag="VF089_09675"
     CDS             complement(2995..3243)
                     /locus_tag="VF089_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019341409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174272.1"
                     /translation="MDWLDALQWPAMVVTVSAAWLIGSQRKIRRMIGFWCFLLSNALW
                     IIWGWHARAWAMVILQIILAGMNIRGVNKNETDNPAEK"
BASE COUNT          756 a          919 c          884 g          728 t
ORIGIN      
        1 agaaacggcg ggccgtttga gcaacagcgt caagttgaac ttgccattca aaacgcccta
       61 cgattggcag gcgattatcc agtactttca gtcacactcc atcccgggag tcgaacgggt
      121 aacgggcgat tccttcgcgc gcgtgtttcg ggtaggaaat gaagtcggct tcgtcgaggt
      181 tcgcccgctt ctcgccaaat cgcagctaaa agtctgggtc gtgtctgaag atcccgaaac
      241 aacatccgaa acggcgcgcc gggttcgaaa aatgttcgac ttggattgcg atcctgtgct
      301 gattgcgaag agcttcgagc gcattcccgt gctggcggac ctatgccgcc gctttccggg
      361 tctcagagtg gctcgcggct gggactgctt cgaaaccgcc atatgtacga tcttggggca
      421 actcgtttct gccgggcaca gagaacgtct gatcggtcaa cttgtaagca actacggtga
      481 ggaggtagtc gatccctcga catcaaaaag agcatactta tttcccgccg ccggagtcct
      541 ggctagctca gatctaagcg cgattggaac aaccaccgcg aggcgagagg cgatccggga
      601 cttcagccgg cgcgtgctga gccgggccat ttctctcgcc gaagaccagg atcccgccgc
      661 gtttcgcgag gcgcttctcg aaacaaaagg gctgggtccg tggtcggcgg attatatcag
      721 acttagaact ggcgataacg atgcgttccc caaaaccgat ttgattctca agagagttct
      781 cgatcttcac cccgatttgg atttagaacg gattaagcct tggagatcgt atgcggcggt
      841 ttatctatgg aaggagtttg cccatacact gtcgaggagc agaaacagga aacggcacga
      901 tgctatccta taaagtgatg gaatcaccgg tgggcaaact aaagctggtg gccaacgcca
      961 atgctttggt cgcaatactc tgggaacggg aacgaccaaa ccggatcaag ctcgcgatac
     1021 tcaaatccga tccgcaacag cctatcctgc tagaggctga gcggcagcta aaggaatatt
     1081 tcgccggcac aagaaacgag ttcaatcttc cgcttgagcc ggctgggacc gaattccaga
     1141 aaaaagtctg gcaagccttg cgagaaatac cgttcggcca aaccaggagc tatctggatt
     1201 tggccaagag catcggctcg gccaaagccg tgcgcgcagt cggcgccgcc aacggcaaaa
     1261 atccgttgtc gatagtcgtg ccgtgtcatc gtgtcctcgg cggggatgga gcgctgacgg
     1321 gctttgccgg cggtctcgaa gtgaaggcaa ggctattggc gcacgaaggg tgcggtcgct
     1381 tttccgcgcg cggagaggaa aaacttacat gagcagccca gcgacgattc gagagcagac
     1441 aatcgattgg gagcgggcat gcgctgctct cgacgaacgc ggctttgcgc tgcttccccg
     1501 cgtgctcgta agaaaaacct gtcaggacat tgcccggtat tacaacgacg atcatcggtt
     1561 ccgctcgcga atcgagatgt cccgttattc atttgggcgg ggagagtata agtatttcgg
     1621 ttacccgcta cccgcaatcg ttcaacaact caggtcttcc atttatccgc atctcgcccc
     1681 gcttgccaat cgatgggccg accggcttgg aagcaaactg ccgcgctatc ctgaaacact
     1741 tgcggacttt ctcaaacagt gtcatcgcgt tggccaaaac cggccaacgc cattgctgct
     1801 aaaatacggc cccggtgatt tcaactgcct gcaccaggat ctttacggtg acagtgtgtt
     1861 tccctttcaa ctcacctttt tcttgagcca gcgccaatct gatttcggag gcggtgaatt
     1921 cgtccttgcc gaacagcaac cacgccggca gtctcgggtc gaagtgctca cacccgacca
     1981 gggcgacgcg gtaatctttt cagttcaaca tcgacccgtt cgaggcgcgc gcggctacta
     2041 tcgggccaac cttcgtcatg gcgtcagcac cattcatttc ggcgagcgct ataccctggg
     2101 aatcattttc cacgacgcgc agtgagactg accgggtgct cgctcgactt gataagagaa
     2161 gcgatctaaa agcgtgcacg gccggtcatg gcgttgtcgg aagatttctt acttgttgca
     2221 gctgatccct aacacctata cgccgactta ttctctggaa gcacgttgga ttggctcgtg
     2281 aagtttatga ttcccgtgga caatggcctg agcccgagac attcgttgtg tgtcaaatct
     2341 tacttgaatc gaaatgttgg tcgtcgccgc tgattgtaat gaatgcttcg aatcggctca
     2401 ggatctccgg aggttcgcgc gccaggatca gcaatgagcg gtgcttttgt ttgagaaagt
     2461 catgctccac tgcatgatcg agaaactcga tcagaggatc gtagtagccg ccggtgttca
     2521 aaaggccgca aggtttgcgg tgaatgccaa gctgagccca gctaagcact tcgaagaact
     2581 cctccatcgt gccaatgccg ccgggcatag cgatgaagcc gtcggataac tccgccatca
     2641 tggctttgcg ttcgtgcatc gactctacga tgcgcagatc cgagagaccc agatgcgcga
     2701 cctctctact gaccaacgag tcggggatca cgccggtgac atagccgcca agctccagga
     2761 cagtatcggc gatgaccttc atcagcccaa cgttaccacc accataaacc aggccgtagt
     2821 tgcgcacgac taactcacga cccagttgtc gcgctgcctc cgcatatccc ggatggctac
     2881 cgtccctgga accggtgaat acgcaaattc tccgctccgg gttgttcatt tgcttagatg
     2941 tgggcgactg ctcgccagag cttccaccac ttagttccac aaccgatagg ttaattactt
     3001 ctcagccgga ttatcggttt cattcttgtt aacacctctg atattcatgc cggcaaggat
     3061 tatctggagt attaccattg cccaagctcg agcgtgccag ccccagatta tccaaagcgc
     3121 attgctcaac aggaagcacc aaaagccgat catgcgccga atctttcgct gcgagccaat
     3181 gagccaggcg gcactaaccg tcacgaccat tgccggccac tgaagtgcat ctagccaatc
     3241 catcgatagt gtctattgct ggagaaaacg acgaagtctt agttcat
//
LOCUS       DASUTG010000423         7023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_658995, whole genome shotgun sequence.
ACCESSION   DASUTG010000423 DASUTG010000000
VERSION     DASUTG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658995"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /gene="smc"
                     /locus_tag="VF089_09680"
     CDS             complement(<1..2095)
                     /gene="smc"
                     /locus_tag="VF089_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX6174273.1"
                     /translation="MRLKRLEIVGFKSFAEKTVVELNPGITAIVGPNGCGKSNIVDAL
                     RWSMGEQSARHLRGHQMEDVVFAGSDSLPATGMAEVSLTFDNEDGRGPVEYSSFSEIM
                     VTRRLFRSGESEYAINKIPCRLKDVIELFLGTGVGSKAYSIVEQGRVDELVNSKPEER
                     RVLIEEAAGTSKYKSRKLVAERKLERTQQNLFRVTDIVREIERQIRTMELQAKKAERY
                     KALKAELREKDLLCASLHRQSLNQEIATQEGYLADVENRLAEYLNSLHGKESESEAVR
                     FSLMELEKEISLQQEKVYQAKMQLQSEEQRIDFFKKDLTQLSENEAETLLALSQLDDK
                     LKTLAREIEELNKAKESFIQLSLFEESFLRENETELEKLQAEIQALQSDLETEKAALI
                     DITNHLVYLKNDLAAKERRRDETSRALERNCDEHATAVASLELIESKRNEAERALEVS
                     LQQAGERNLDFAQATALIQSLSRSKEEQEKTIEALKGRIQESRSRLLSLEDLQRNYEG
                     YQEGVRAIMLKRQEEAATDGICGLVTEVIEAPVLYEKALTAVLGDRLQYMIVKGHDEG
                     IEAIEFLKQKASGRGSFIPVQMTRKSNRPLPLGEAEVVAPLLELISVKEGYRDVAEYL
                     LSDVVVVQDLKSGLALWHRNGYYSTLVTPEGEVIDPMGVVTGGSEESLEGSFLSQRRR
                     MKELRILLTELESEMQ"
     gene            complement(2243..2875)
                     /locus_tag="VF089_09685"
     CDS             complement(2243..2875)
                     /locus_tag="VF089_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6174274.1"
                     /translation="MNYSATIPVYSVLNTKVLILNRSYLPIHVTSVRRAFTLLYQGIA
                     KAVNEQYQTFDFESWSDLSVTVHDESVGMVNRVIRVPRVILLVSYDRMPKRQVRFSRY
                     NIYARDKCTCQYCGQRRPRHELNLDHVVPRTQGGTSTWENVVCSCQECNRLKGGRTPQ
                     QAGMTLARRPVRPQWTPFMQETFSLSRYKEWLPFLNTVDASYWNTELLER"
     gene            3111..4622
                     /locus_tag="VF089_09690"
     CDS             3111..4622
                     /locus_tag="VF089_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX6174275.1"
                     /translation="MKQQIFINSSPQESRIAIMEDGVLAEFLIERKEERGIAGNIYKG
                     KVERVLPGMQAAFVDIGMDKAAFLHASDFFSVPEDVQLISQSGDDVEFDDVPKPAPSR
                     RLPLEKQVSRGEDILVQVAKDPLGTKGARITSHVSLPGRYMVFMPSTKHIGISRRIES
                     EEERKRLKEIAQSLGTTEGGFILRTASEGQSKREIQRDLRFLTKLWKRIQEKAEKAPA
                     PALIHQDLDLITRTIRDFFTTDTEQVVIDSAKDYRRTVDFVGHFMPRLKSKLVLYSEK
                     ELLFDRHGIEEKIEKALDRRVWLRSGGYIIIERTEALTAIDVNTGRFVGKRDQEETIL
                     KTNMEATSEVVRQLRLRNLGGIIIIDFIDMEKEGNRKKVYEALRTALKKDKARTNILK
                     ISELGLVEMTRQRTRESLENQLLSPCPHCDGRGRIKSSVTIAYDILRAIKKQQTSLEN
                     GKNIVLRLHPTVANFLYDEKANSLEALEREINHKIIIKVSEDLRHQQYEISTT"
     gene            complement(4624..5271)
                     /locus_tag="VF089_09695"
     CDS             complement(4624..5271)
                     /locus_tag="VF089_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6174276.1"
                     /translation="MDPQNLLKVIQTRRSVRVYKKGKVTDEQLAFILEAARWAPSGAN
                     TQPWELVVTRDREKMRRIRQIYDNEWRQRKREDPINYKGLKKDYVGDVSIIVLVCGDA
                     RTKQVYLTTRQPADREKLFQASIANAVQQMMLAAASMNLGTVWVSVREEVEPELRELF
                     KVPTSLRLLWVVPIGHARSWPAAKPRRNPAAFTHLEVYDSSKLRKESEIRPWPKS"
     gene            complement(5388..>7023)
                     /locus_tag="VF089_09700"
     CDS             complement(5388..>7023)
                     /locus_tag="VF089_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6174277.1"
                     /translation="VLAMFWVSVQKGAVGTPSAIYTIGNYIEVFSDPFVLRVAANTIV
                     FTITATAVALLFGLPIAWLAERSDLPGKTLIYAIMTLGLLIPGIYTAMGWTLIAHPRI
                     GILNRWLVDIFSLNDAPINIATPIGMGFVQGLSLSALAFILTAQMFRAMNPTLEEAAR
                     VHGMGYGKILWRVTLPLARPGILAAVIYILTIGIATFDIPAILGLGNRVYMLSTFIYL
                     KVHPQGAGLPEYGITGVVGAFMIIVAVLLTLWYSQVLRQGHRFEVVTGKGYKPALLPL
                     GKWSIMAWCFIGAYTFISKLLPLLLITYAALTPYFVPPSLAMLGNLSFSHFYGMDWEL
                     VTRGLANTALLVAVVPLAVLLLAFSISWLIVRSRSRARYALEFGAFLPHALPEVILAI
                     GALLLSLFVFGNSIPLYGSVWLIAIVYVIARLAFATRALNGSLLQIHRELEEAAFVAG
                     LSTLRTAWHVLFPLVRPTLFSVWIWSALLVYRELTVAVFLAVHDNITLPAVIWSYWYS
                     GSINKASAVTLLMTVVLAPLILAAWWYGRRSQISAQ"
BASE COUNT         1678 a         1867 c         1850 g         1628 t
ORIGIN      
        1 tttgcatctc actttcgagc tcggtgagca gaatccgaag ctctttcatg cgccggcgct
       61 gacttaagaa gctgccttcc agggattctt cgctgcctcc ggtgacaact cccatggggt
      121 cgatgacctc tccttcggga gttaccagcg tgctgtaata accgttgcgg tgccacaagg
      181 ccaatccgga tttcagatcc tgcacgacaa cgacatccga caggagatat tcggccacat
      241 cacgatagcc ttctttgact gaaattaatt ccaacagcgg agcgaccacc tccgcctcgc
      301 ccaacggtaa agggcgatta gatttccggg tcatctggac cgggatgaag ctgcctcttc
      361 cggaggcttt ttgcttcaga aattcaatcg cctcgatgcc ttcgtcatgc cctttaacaa
      421 tcatgtactg caggcggtct cccaacactg cggtcagcgc tttttcatag aggaccggag
      481 cctcaatcac ctcagtcacc aaaccacaga tgccatcggt ggcggcttcc tcttgccgct
      541 ttaacataat cgctcggacg ccttcttggt aaccttcata gtttctttgt agatcttcga
      601 gagacaataa tcgggagcga ctttcttgaa tccgtccctt gagagcttcg atcgtttttt
      661 cctgctcctc tttgctccgg cttagacttt ggatcagggc ggtggcttgt gcaaagtcaa
      721 ggtttcgctc tccggcttgt tgtagagaaa cctctagagc tcgttcggct tcattcctct
      781 tgctttcgat gagctccaaa gaagcgacgg cggttgcgtg ctcgtcgcaa tttcgttcca
      841 gagcgcgact ggtttcatcg cgccgccgtt ccttggcagc cagatcattc tttaaataga
      901 ccaggtgatt ggtgatatcg atcagcgccg ctttttcggt ctcgaggtca gactgcagcg
      961 cctggatctc ggcttgaagt ttttcgagct cggtctcatt ttcgcggaga aagctctcct
     1021 caaaaaggga gagctggatg aagctttctt tggctttgtt gagctcttcg atctctcggg
     1081 cgagagtttt gagcttgtca tcgagttgcg atagggcgag cagggtctca gcttcgtttt
     1141 cgctaagttg cgtcaaatcc tttttgaaga agtcgatgcg ctgctcttcc gattgaagct
     1201 gcatctttgc ttgataaacc ttctcctgtt gtagactgat ttctttctcc aactccatca
     1261 atgaaaagcg gaccgcctcg ctctcggatt ccttgccgtg caaactgttc aaatattcag
     1321 ccaggcgatt ttccacatca gcgaggtagc cttcctgggt ggcgatttcc tggttcagcg
     1381 attgccgatg caggcttgcg catagcagat ccttttcccg gagttccgct ttaagggcct
     1441 tgtagcgctc tgccttcttt gcctgcagct ccattgtgcg gatttgtcgt tcaatctccc
     1501 gtacgatgtc ggtaacccga aacaggttct gctgcgtgcg ctcgagcttc cgctcggcta
     1561 ctagcttgcg gctcttgtac ttactcgtgc cggctgcttc ttcgatcaac actcggcgtt
     1621 cttcaggttt tgaattgacc agctcatcga cccggccttg ctcgactatc gaatatgcct
     1681 tgctgccgac gccggtacct aaaaacaatt cgatgacatc tttcaggcgg catggaatct
     1741 tattgatggc gtattcagat tcccccgatc ggaagagccg tcgggtcacc atgatttcgc
     1801 tgaagctact gtattcaacc gggccccgcc catcttcatt gtcgaaggtt agcgaaactt
     1861 ccgccatacc ggtggcggga aggctatcac tgcctgcgaa gaccacgtct tccatttgat
     1921 gaccgcgcag gtgacgcgcg ctttgttcgc ccatggacca gcgcaaagca tcgacgatgt
     1981 ttgatttgcc acagccattc gggccgacga tcgcggttat tccggggttt agttccacca
     2041 cggttttttc ggcgaacgac ttaaagccca cgatctcaag gcgtttcagg cgcataatcg
     2101 tttggtgtca gctacttacg gaacggcgga gtgaatcaat ggattgcttg gcactatagt
     2161 ccatcaggcg gataactgtc aagaaaattg accatataaa gtggctgaag acgccattcg
     2221 ctactatata taggagacgg ggttatcttt cgagcagttc ggtgttccag tatgaggcgt
     2281 cgacggtgtt caagaagggc agccattcct tataacgaga aagactgaag gtttcctgca
     2341 taaaaggggt ccactggggt ctgaccggtc gccgggccaa tgtcataccc gcctgttgcg
     2401 gagtacggcc tcccttcaac cgattacact cttgacatga gcagaccaca ttttcccatg
     2461 tggatgtgcc cccttgtgtt cggggtacga cgtggtcaag attgagttcg tgccgcggcc
     2521 gtctttggcc gcaatactgg caggtgcact tatcgcgggc ataaatgtta tatcgactaa
     2581 agcgaacctg acgcttgggc atacgatcat aactgacgag cagaatcacc cggggaaccc
     2641 ggattacccg gttgaccatg cccaccgatt cgtcgtgcac agtgaccgac agatcgctcc
     2701 agctctcaaa atcgaaagtt tggtattgct catttactgc tttagcaatg ccctgataga
     2761 ggagggtgaa ggcgcggcgc accgaagtga catgaatcgg caaatacgag cgattgagaa
     2821 tcagtacctt ggtgttgaga acggaataaa ccggaatagt agcggaatag ttcatttaag
     2881 ctttggcatg gttcgaggat tacgtcccca tcatagctaa aatacgaggc aggtcacaca
     2941 atgtcaaacg agcgcaaaat tgtcgccacg ctgcttgaac tcgctattga attactctat
     3001 aaagacacca gccgccatcg atgacttttg ggacttccga tgccgaacgg caccccgttc
     3061 ctgcattccc gcctcttctg cacatcgcgt cgaattctag taggctatct atgaagcaac
     3121 aaatttttat caactcgtct cctcaggaga gccgcatcgc aataatggaa gacggtgttc
     3181 tcgctgagtt tttgattgaa cgcaaagagg agcgaggcat tgcaggtaac atttacaagg
     3241 gcaaggttga acgggttttg cccggcatgc aggcggcgtt cgtcgatatc ggcatggata
     3301 aagccgcctt tctacatgca tcggatttct ttagcgttcc cgaggatgtg caattaatct
     3361 cacagtccgg tgacgacgtc gaatttgatg acgttcctaa gccggcgcct agccgccgac
     3421 tgccgcttga gaagcaggtt tcacggggtg aagacattct agtccaagtt gccaaggatc
     3481 cgctggggac aaagggggcc cggataacat ctcatgtttc gctcccgggc cgttatatgg
     3541 tctttatgcc gagcaccaag catatcggca tctccaggcg aatcgagagc gaagaggaac
     3601 gcaagcgact caaagagatt gctcaatctt tggggacgac cgaaggcggt ttcatccttc
     3661 gaaccgcctc cgaagggcaa agcaaacggg aaatccaacg agatctcagg tttctgacca
     3721 agctgtggaa acgaattcag gagaaagctg agaaggctcc ggctccggca ttgattcacc
     3781 aggacctcga cttgatcacg cgcacgatac gggatttttt tactaccgat accgagcagg
     3841 tggtcatcga ttcggccaaa gattaccggc gcactgtcga cttcgtcggc cattttatgc
     3901 ccagactcaa atcgaagctc gtgttatatt ctgaaaaaga gctgcttttt gatcggcacg
     3961 gtatcgagga gaaaatcgag aaggctctgg accggcgcgt ctggctgcgt tcgggcggtt
     4021 acattatcat cgagcgtact gaggcgctta ccgctatcga cgtcaatacc ggacgcttcg
     4081 tcggcaaaag agatcaagaa gaaaccattc ttaaaaccaa tatggaagcc acttcggagg
     4141 tggtgcgcca acttaggcta agaaacctcg gcgggatcat cattatcgat tttatcgata
     4201 tggagaaaga aggaaaccgc aagaaggtct atgaggcgct caggactgcg ctcaagaaag
     4261 ataaagcccg taccaacatc ctgaagattt cggagctcgg tttggtcgaa atgacccggc
     4321 aaagaacccg cgagagtttg gaaaaccaac tgctaagccc atgccctcat tgtgatggcc
     4381 ggggccgtat caagtcatcc gtgacgatcg cgtacgatat tttacgagcg atcaagaagc
     4441 agcagacgag cttggagaat ggcaagaaca tcgtgctgcg gcttcatcca accgtcgcca
     4501 attttcttta tgatgaaaag gccaacagcc tagaggcgct ggagcgggaa atcaatcaca
     4561 aaatcatcat caaagtgagc gaagacttac gccaccagca atacgaaatc tccacaacct
     4621 gatttagctc ttgggccaag gtcggatttc ggactccttc cttaatttgc tcgaatcgta
     4681 aacttccaga tgcgtgaatg cggcgggatt acgcctgggc tttgcggcgg gccaactgcg
     4741 cgcatgccct atgggcacga cccagagaag gcgcagactc gttggaactt taaacaactc
     4801 cctgagctcg ggctccacct cctcgcgcac ggaaacccat acggtaccca aattcatact
     4861 cgcggcggca agcatcatct gctgcaccgc gttggcgatg ctggcttgga aaagcttctc
     4921 gcggtctgcc ggctgtcgcg ttgtcagata aacctgtttg gtccgggcgt cgccgcagac
     4981 cagaacaatg atactgacat caccaacgta atctttcttc aaacctttgt agttgatcgg
     5041 atcttcgcgt tttctctggc gccattcatt gtcatagatc tgccgaatac gtctcatttt
     5101 ctcgcggtcg cgagtcacca ctaattccca gggttgagtg ttggcgccgg aaggcgccca
     5161 acgagctgct tccaggataa acgcgagctg ctcgtcagtg actttgccct ttttgtaaac
     5221 gcggacgcta cgccgtgtct ggatgacttt aagtaaattc tggggatcca tacgtgaaac
     5281 ctttatttta acctcgaaaa aaatcgcatc aaataccgaa agtagaaaga gatagctggc
     5341 aaaacactat tcagctttcc tcttttattc tttagttctt gcgggtgtta ctgcgcggaa
     5401 atctgactgc gccggccgta ccaccatgcg gcgagaatca gcggggcaag aaccactgtc
     5461 atgagcaacg tgaccgccga cgccttgttg atactgccgc tgtaccagta gctccaaata
     5521 actgcgggca aggtgatgtt gtcatgcact gcgagaaaaa ctgccaccgt caactcgcga
     5581 tacactaaca gcgcggacca gatccaaacc gaaaacaagg tagggcgaac cagtgggaat
     5641 agcacgtgcc aggcggttcg taaggtggaa agacctgcca cgaaagcggc ctcttcgagc
     5701 tcccgatgaa tttgcaagag cgaaccgttg agggcgcgag tggcaaacgc gagccgggca
     5761 atcacgtaaa cgatggcgat gagccagacg gagccgtaga gcgggatact gttgccgaat
     5821 acgaacaagg acaggagcaa cgcgccgatc gcaaggatta cttcaggcaa ggcatgaggc
     5881 aggaacgcgc caaactcgag tgcgtaacgc gcgcggctgc gcgagcgcac gataagccag
     5941 gagatactga aggccagcaa caacaccgcc agaggaacca ctgccaccag gagggccgtg
     6001 ttggccaagc cgcgcgtcac cagttcccaa tccatgccgt aaaaatggct gaacgaaaga
     6061 tttcccagca tcgccagaga gggcggaacg aaatacggtg ttaaagcggc gtatgtaatc
     6121 aaaagcaatg gcaggagctt cgatatgaac gtataagcgc cgataaagca ccaggccatg
     6181 atggaccact tgccaagcgg caagagcgcc ggcttgtaac ctttgccggt gacgacttcg
     6241 aaccgatgac cctgccgtag cacctggctg taccagagag tgagcagaac ggcgacgata
     6301 atcatgaacg cgcccacgac tccggtaatg ccgtattccg gcaggccggc cccctgggga
     6361 tggactttta agtagataaa ggtgctgagc atataaacgc ggttgcccaa accgaggatc
     6421 gccgggatgt cgaacgtggc gatgccgatt gtcagtatgt agatgaccgc cgctaatata
     6481 ccgggccgcg ccaacggcag ggtcacgcgc caaaggattt taccgtaacc catgccatgc
     6541 acgcgcgccg cttcctcgag agttggattc atggcccgaa acatctgtgc cgtgagaatg
     6601 aacgccaatg cagagagact caaaccttgc acgaatccca taccgatggg tgtggcgata
     6661 ttgattggcg cgtcattcag gctgaagata tcgacgagcc agcggtttaa aattccgatg
     6721 cgcgggtgag caatcagtgt ccatcccatc gccgtgtaga tgcccggaat cagcaggccg
     6781 agtgtcataa tcgcgtagat cagcgtcttg cctggaagat cgcttcgttc tgcgagccag
     6841 gcgatcggca atccgaatag cagcgccacc gccgtcgcgg tgattgtgaa aacaatcgta
     6901 ttggcagcga cgcgcagcac aaaaggatcg ctgaaaacct cgatataatt gccgatcgtg
     6961 tagatcgcac tgggcgtgcc gacggcgcct ttctgaacac tgacccagaa catcgccaac
     7021 acc
//
LOCUS       DASUTG010000424         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_214407, whole genome shotgun sequence.
ACCESSION   DASUTG010000424 DASUTG010000000
VERSION     DASUTG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214407"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF089_09705"
     CDS             complement(<1..521)
                     /locus_tag="VF089_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174278.1"
                     /translation="MRNYKNYLHTLFKRSFARIGFAVLFVVLGLSSTAFADIVTEWNA
                     IATTVIVTNEKRAAGAAIVDAAYMHAAMYDAINGINPRFSQFAVVPSNVVAGSSEAAA
                     GAAAAYTVLVHLFPNQKPFLDEKYAQSLAQISDDQSKTDGIAIGTEVAEKLIKLRAGD
                     GWNAPVSITPGNGP"
     gene            1284..2498
                     /locus_tag="VF089_09710"
     CDS             1284..2498
                     /locus_tag="VF089_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174279.1"
                     /translation="MVAIVTGRLFQLREKLIVRVALVDAISGNQLWGEQYDRNSADLI
                     EIQVEISAEVAKQLRLRLTGEDERRLAKRSTDSHEAYRAYLKGRYFWSKRPQAGFMKG
                     LDYFRQAIDLDPSYALAYAGLADAYGVLGSWEAGFLPPREAATKARAAAVRALEIDED
                     LAEGHTSLAYVALHYDWDWTSAEQGFKRALELNPSYVHAHHWYSHLCVVTGRIKKAYE
                     VSVRALELDPLDLIVNVHLAWHYWITRDPSRAIEQSLRTRELDPDSLWCHFFCGLAYE
                     EQGRYVEAVAQLKRASELSTVTTFALAGQAHASALAADKPSAEAALRKMEQPTDDRYI
                     PAYDKAIICVGLGDRERAMRYLLEAFEEHSSWMAYLNVEPRLDPLRDDVRFIELLKRV
                     GVKTPSETGHLA"
     gene            complement(2613..>3457)
                     /locus_tag="VF089_09715"
     CDS             complement(2613..>3457)
                     /locus_tag="VF089_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174280.1"
                     /translation="SRSLVNTFPYFTATDRSMADNFFRPGFTQGVWANGELGKGLNYI
                     AFVGNALNSINISAAKIDTNLLVSGSVWWEPLGRYAEPGKSVNMYDDYFAKKKTRIRI
                     GTSFTRSREDRFSNLDQSSPDNTALFNSDGVLTFSTGAFAPGVTVEKALYQMWAMDGG
                     IKYNGLAINGQYFMRWLSDFEADGPIPVTSTFDHGYELSAGHFVIPKKLMVYVRGSQV
                     FGQFRNSHEYAGGVKWHFLPTERLWLNAELMRVNRSPYNGAFTPYTAGMNGWAPMIQT
                     IIAF"
BASE COUNT          829 a          879 c          935 g          814 t
ORIGIN      
        1 ggaccgttgc ccggtgtgat agaaactggc gcattccagc cgtcaccagc acggagtttg
       61 ataagcttct ccgctacttc tgtaccaatt gctatcccat ccgtctttga ttggtcatca
      121 gagatttgtg cgagcgactg cgcgtacttt tcatcaagaa aaggtttttg atttgggaat
      181 aagtggacga gcacagtgta agcggctgcc gcaccggccg ccgcttctga gcttccggcg
      241 acgacattcg atggaacgac ggcaaactga ctaaaacgag gattgatgcc attaattgca
      301 tcgtacattg ccgcgtgcat gtatgccgca tcgacgatcg ctgcacccgc ggctcttttt
      361 tcgttagtga cgatgaccgt ggtcgcgatc gcattccatt cggtaacaat gtctgcaaag
      421 gcagtactac tcaggcccaa cacgacaaac aagaccgcaa agcctattcg cgcgaagctg
      481 cgtttgaaca aggtgtgtag ataattttta taatttcgca tgatcattgg ctcctttagt
      541 tattctcggg accatcccgg cccgtgggtt ttgctgatga agggcctcgc aactagaatg
      601 gcgcctccac cggctggcat gctcaagtta agccagatgg cgagaagtag tcctttgcag
      661 cttcttattt gtttcttatc tggtcgttaa cggggtttga ggtcaaagtg gattacaggt
      721 ttgagttcgg ccacttttac atcgacacaa ttgaacgcct gctaattcgt gacggcgagc
      781 gcatcacgct gaaaaacaaa gcgattgagg tgttgctgct cctgctcaaa aagagtcccg
      841 ccgtggtcga aagggagtcg ttcatgaatg agatctggcc ggataccttt gttgaggaag
      901 gcagtctaac tgtcagcatc tctgagatca ggaaggctgt gggcaggggc tcggatggtc
      961 ggcactacat cgaaaccctt cctggtcgcg gctaccgctt cactccagaa ccaaaccgag
     1021 tgataattaa cccttccgtc cagcctcctc acaaattcga gactagtcag tcaccgcaat
     1081 ctacgactga acgcgctatc gctgttcttc ctttcatcaa caccagctca gatccttcgc
     1141 tggattatct tgcggacggc cttgctgaaa cgattatcaa tcggctctca catctcaata
     1201 agttgcgagt aaaagccagc gggagcagtt ttcggtttag aggtcgagag gcagcctggt
     1261 ccgaaatcag tcgggaactc ggagtggttg caatcgttac tggtcgactg tttcaactcc
     1321 gcgagaaact gatcgtacgc gtcgcacttg tggatgctat tagtggaaat caattgtggg
     1381 gcgagcagta cgatcgtaac tccgcagatt tgattgagat ccaggtagag atatcagccg
     1441 aagttgcgaa gcagctgcga ctacgtttga cgggtgaaga cgagcgacgt ttggcaaaac
     1501 ggtcaacgga ctctcatgaa gcttatcgcg cctatttgaa aggacgttat ttctggtcca
     1561 agcgcccgca agcaggattc atgaaagggc tcgattactt tcgtcaggct attgacctcg
     1621 atcccagtta tgctcttgct tatgcgggtc tggcagatgc gtatggcgtg ctcggctctt
     1681 gggaagccgg cttcctaccg cctcgggagg cggcaacaaa agcgagggcg gcggcagtca
     1741 gagctctgga aatagacgag gatctcgcag agggtcatac atctcttgcc tatgtggcgc
     1801 tgcattacga ttgggattgg accagcgcgg agcagggttt caagcgcgcg ctggaactca
     1861 accccagtta tgtccacgct catcactggt actcccatct atgcgtggtc actggccgga
     1921 tcaagaaagc atatgaagtc agtgtacgag cactcgaact ggacccgctt gatctaatag
     1981 tcaacgtaca tttggcatgg cattactgga ttacgcgcga tccatccagg gcaattgagc
     2041 aaagcctgcg gacgcgggag ttggatcctg actcactgtg gtgccacttc ttctgtgggc
     2101 ttgcttacga agagcaaggt cggtacgtgg aagcagttgc gcagttgaaa cgcgcgagtg
     2161 aactgtctac tgtcacaacg tttgcactgg ctggacaggc tcacgcttca gcactggccg
     2221 cggataaacc gtccgcagaa gctgcgttgc gaaaaatgga acagccgacc gatgatcgtt
     2281 acattcctgc gtatgacaag gcgattattt gtgtagggct tggtgatcga gagcgagcaa
     2341 tgaggtattt gctggaggca tttgaagagc attcaagctg gatggcttac ttgaatgtcg
     2401 agccccgact agatccatta cgagacgacg tacggttcat cgagctgctg aaacgagtgg
     2461 gggttaaaac gccctccgaa acaggacatc tcgcctgaaa catacacgtc cacacagaaa
     2521 tcccactcgt caccacacga caatcaattg gaatccaggg aactcattgc gagcatggaa
     2581 aaagaaaaga aggccgcaga aactggcgat cgtcaaaaag cgatgatcgt ctgaatcatc
     2641 ggcgcccagc cgttcattcc cgcagtatac ggagtaaagg ccccgttata aggtgatctg
     2701 ttaacccgca tcagctcggc gttgagccat aaacgctcgg tcggtaggaa atgccatttg
     2761 acgccccccg cgtactcgtg cgagttgcga aactgtccaa acacctgcga gccgcggaca
     2821 tacaccatca gcttcttagg aatgacaaaa tggcccgccg acaattcata gccgtggtcg
     2881 aacgtcgatg tgaccggaat gggcccgtcc gcttcgaaat cgcttagcca acgcatgaag
     2941 tattgcccgt tgatcgcaag tccgttgtat ttaatgccgc cgtccatcgc ccacatttgg
     3001 tagagagctt tctcgacggt cacacccggc gcaaacgctc cggttgagaa cgtaaggacg
     3061 ccgtcggaat tgaacagcgc agtgttatca ggacttgatt gatcgagatt tgaaaaacgg
     3121 tcctcgcggg atctcgtaaa cgaggtacca atgcgaatgc gagtcttttt cttggcaaaa
     3181 tagtcgtcgt acatgttgac tgatttgccg ggttccgcgt atctccccag cggttcccac
     3241 caaacactac cggaaacgag cagattggta tcgatcttgg cggccgagat atttatgctg
     3301 ttgagagcgt tgccgacgaa cgcaatataa ttcagcccct tcccgagttc tccgttggcc
     3361 catacgccct gggtgaagcc ggggcggaag aagttgtccg ccatgctcct atcagttgct
     3421 gtgaaatagg ggaacgtgtt gacaagcgat cggctgc
//
LOCUS       DASUTG010000425         4355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1400447, whole genome shotgun sequence.
ACCESSION   DASUTG010000425 DASUTG010000000
VERSION     DASUTG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400447"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..384
                     /locus_tag="VF089_09720"
     CDS             202..384
                     /locus_tag="VF089_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174281.1"
                     /translation="MWFSLLWCILAVMLFITILGVPFALMVLLAAGVWAIYRIARGSL
                     SLRDRRPMYTSGGVRI"
     gene            complement(445..930)
                     /locus_tag="VF089_09725"
     CDS             complement(445..930)
                     /locus_tag="VF089_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174282.1"
                     /translation="MIGEWTAALEATQIATALRNSIWSYPLVNTAHIFGVALLIGSVV
                     PLDLRLLGLWGSVPLAPLWTVLTRTAGIGLVLAIIFGAFLFITRATEYAASNLFIAKM
                     FLVFLGTANTLMLRMPTQAPESHIASIRVKPSARLRVAAGISLAAWLSALTLGRLIGY
                     F"
     gene            complement(948..1328)
                     /locus_tag="VF089_09730"
     CDS             complement(948..1328)
                     /locus_tag="VF089_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009818638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX6174283.1"
                     /translation="MKRAFKSSLALVAMLTAVFLTQPVWAHHGWGWATEEEFQLTGEI
                     TAVRLGNPHGEVTVDVKGVGWIVEVGQPWRNEKAGLTQKLLSNGQVITAHGHRSAKKG
                     QRLMKAERVVINGRSYNLYPDRES"
     gene            complement(1521..2276)
                     /locus_tag="VF089_09735"
     CDS             complement(1521..2276)
                     /locus_tag="VF089_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6174284.1"
                     /translation="MRYELYYQPSIQGRGEFIRLALEEAGADYLDVARDPNFGRPGIM
                     KFLEDPALEHPPFAPPFLKAGELLISQTANILQFLAPRLGLVPEKEESRLWAHQLQLT
                     VADWLYETGQTHHPIANVLYYEEQKDEAKKRAAHFTAIRIPKFAGYFERILKLNANGG
                     DFIFGEEVSYVDLSLFQMIEGLRYAFPKTMAQVEPQHPKLVTLHDRVMARARIAAYLA
                     SPRRLPFNEQGIFRHYPELEEAQAQSDKANKPD"
     gene            2774..3832
                     /locus_tag="VF089_09740"
     CDS             2774..3832
                     /locus_tag="VF089_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6174285.1"
                     /translation="MRYKTFGRRAGLRVSELALGTANFGTRWGHGAARGDAKEIFDAY
                     VEAGGNFIDTADSYQFGESEELVGEFIRADRDYFVVATKYTLGTNASDGISRTGNSRK
                     NMTRSVEASLKRLKTDRIDLYWAHFADGMTPMEEIIRAFDDLMRGGKIHYAGLSNFPA
                     WRISRADLLAEIRGWGPIAGIQVEYSLAQRTAERELLPMAEALGLGAALWSPLGGGFL
                     TGKYRIRGEGRINSFLARLVHTEKTARETAILDGVMAIAKEIDRSPTEVALAWILHKA
                     RLSTTAWIPILGPRSHEQLAGTLTALEVNLSQEQIGRLDEASQIAFGTPHEQTYGSAA
                     AIAGGKPELLQPRIIPVA"
     gene            3987..>4355
                     /locus_tag="VF089_09745"
     CDS             3987..>4355
                     /locus_tag="VF089_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6174286.1"
                     /translation="MKLISVNVGLPRVVTLNGDPVSTGIFKEPVAGRVMLRTLNLDGD
                     RQADLSVHGGPSKAVYVYPSEHYDYWKQELPGVKLPWGMFGENFTSVGLFESEVNIGD
                     RFSVGSAVVMVTEPRMPCYKL"
BASE COUNT          975 a         1172 c         1241 g          967 t
ORIGIN      
        1 gcaagacgac tcaggagcgc attgtcgcgg gacagtaacc gatacgaact tcttaggtca
       61 acttggacac gccggcaaag gcagagctgt cggtgccttc gtctttggcg tgccctcgat
      121 ggttgccgtc gtgattaatt atctgaaagg gtagaaaaca gaggggcctt cccaagtcac
      181 acttcgctgg cagattcgac gttgtggttt tcgctgctct ggtgcattct cgccgtgatg
      241 ctgttcatca ccatactggg cgttccgttc gctctgatgg tcttgttagc ggctggcgtg
      301 tgggccatct accgcatcgc tcgcggctcg ctctccctgc gagaccgaag accgatgtat
      361 accagcggtg gagtacggat ctgaccagca agaacaattg actccatcct ggatcaaaga
      421 gttccgttct gttcggtgaa gttttcagaa gtagccgatt aggcgtccca aagtcagtgc
      481 cgataaccac gcggcgagcg atattccagc ggcaactcgt aaccgagccg acggtttcac
      541 tcggattgat gcgatgtgcg actctggcgc ttgggtcggc atgcgcagca tcaaagtatt
      601 cgccgtgcct aagaaaacaa gaaacatttt ggcaataaat aagttagaag ccgcgtactc
      661 cgtggcgcga gtgatgaaca ggaacgcccc aaagattatc gccagaacca ggccaatgcc
      721 ggcggtgcgc gttagcactg tccagagcgg tgcaagcggc accgaacccc ataggccgag
      781 aagccgaaga tccaatggca cgactgatcc gatcagaagc gccaccccaa agatgtgcgc
      841 cgtattgact aggggatagc tccagatcga gttgcgcagc gctgtcgcga tctgggtcgc
      901 ttccagcgcg gcggtccatt caccgatcat ccttgccggc gggctaatta tgattcacga
      961 tcaggataca gattgtagct cctgccattg atcaccacgc gctcggcctt catcagccgc
     1021 tgccccttct tggcggagcg atggccatgc gcggtgataa cctgtccatt actaaggagc
     1081 ttctgcgtta gtccggcctt ttcgttacgc cacggttgcc ccacttcgac gatccacccc
     1141 acacccttaa cgtccacagt cacttcacca tgagggttgc ctagccgtac ggcggtgatc
     1201 tcgccggtta gctggaactc ctcctcggtc gcccaccccc agccatgatg cgcccaaacc
     1261 ggttgcgtta gaaaaactgc agtgagcatc gcgaccagcg ccagagatga tttaaatgct
     1321 cgtttcatcg tatcctccac tagcttttgc tttatattaa agcttcgacg caccggacgc
     1381 tacgccggtt gaccagaacc acaaaccttc ggtcttcaaa agacaaaaac gaaatggacg
     1441 agcatagctg cacgcgaatt gttgcgccgc gggtgagcag atcactctgc aagatctgtt
     1501 tcaccgacga ttcgctccga ttaatccggc ttattcgctt tatccgactg cgcctgcgcc
     1561 tcttctaatt cggggtaatg gcggaagatg ccttgctcgt tgaacggcag acgccgcggt
     1621 gaggctaggt atgcggcgat ccgcgcccgc gccatgacac gatcgtgtag agttacgagc
     1681 ttcggatgtt gcggttcgac ctgcgccatt gtcttcggaa aggcatagcg caatccctcg
     1741 atcatctgaa acagcgagag atcgacgtag ctgacctcct caccaaaaat gaagtcgccg
     1801 ccgttggcgt tgagtttaag aattcgttcg aaatagccgg cgaatttggg tatccggatc
     1861 gcggtgaaat gcgcggcgcg cttttttgcc tcatccttct gctcctcata gtaaaggacg
     1921 ttggcgatcg gatggtgggt ctgtccggtt tcatacagcc aatcggctac ggttagctgc
     1981 agttggtgcg cccaaagccg gctctcttcc ttttcgggta cgagccctaa tcgaggagcc
     2041 aggaactgga gaatgttggc ggtttgcgaa atcagcagtt caccggcttt caaaaatggc
     2101 ggggcgaacg gtggatgctc caaagcgggg tcctcgagaa acttcataat ccccggacgg
     2161 ccgaagtttg gatcgcgcgc gacgtcgaga taatccgcgc cggcttcttc aagtgcaagg
     2221 cggatgaact cgccgcggcc ttggattgac ggttgatagt acagttcata acgcatgagg
     2281 agtcctccta tgtggatcaa aatattagtc gctcagtaaa cgtacccata aaatgtattg
     2341 atggcagcat tttcagaggt cgacgtatct tctagctatc ctgtcgggct aaacactgac
     2401 cgagaataat acacgccggg ctagatttga aacgtgaagc gtcctcaatc tgatcacggg
     2461 agaatcggcc tccgccttta tcgttggcat agatgactaa cgagatactg tgcaggcttg
     2521 agtgaattat cgcgatgtcc gccggaactt ccataggcca ggcgtttatt ggatggatcc
     2581 gttgaagcgc ctgggtggat cgacgtatgt ttgaaacgac tggcttggaa tggttgttcg
     2641 agcgactcaa ccaagaagct ggcaaggcgg agattttctt gtcgaacgaa gaagtgcgtt
     2701 attcgatctg gtgctaaagt gaaaaattgc ttgcgattca ttcggcgaat gaggtcagct
     2761 gaggagtgaa atgatgcgat acaaaacttt cgggcggcgc gcaggattgc gtgtatctga
     2821 actggctctt ggcaccgcaa acttcggtac gcgttggggt catggcgcag cgcgcggcga
     2881 tgcaaaagaa atcttcgatg cctatgtcga ggctggcggc aactttatcg acactgccga
     2941 cagctatcag ttcggcgaat cagaagagtt ggtcggggaa ttcattaggg cggaccgaga
     3001 ctacttcgtt gtcgccacca aatacacttt gggaacaaat gctagtgacg gcatctcgcg
     3061 aaccggcaac agccgcaaaa atatgactcg ctccgtcgag gcgagtctca agcggctgaa
     3121 gaccgatcgc atcgatctct attgggcgca tttcgccgac ggcatgacgc cgatggaaga
     3181 gattattcgc gcctttgacg atctcatgcg tggcggcaaa atccattatg cgggattgtc
     3241 caactttccg gcctggcgga tttctcgtgc cgatctgctg gccgagatcc gcggctgggg
     3301 acccatcgcc ggcatccagg tcgaatatag cctggcgcag cgcaccgccg agcgggaact
     3361 cttgccgatg gctgaggcgc tcgggttggg agctgcgctg tggtcgccgc tcggcggcgg
     3421 atttcttacc ggcaagtatc gcatcagagg tgaaggacgg atcaattcgt tccttgccag
     3481 gctggttcac accgagaaga cggcgcgtga gacggcgatc ctcgatgggg taatggccat
     3541 tgccaaggaa attgaccggt caccgacgga agtggccctc gcctggatat tgcataaagc
     3601 gcgtctatcc acgaccgcgt ggatcccgat cctcggtccg cgaagccacg agcaactcgc
     3661 cggcacgctc actgcactgg aagtaaactt atcccaggaa cagatcggcc gtctcgatga
     3721 agccagtcag atcgccttcg gcacgcccca cgagcaaacc tatggctccg cggctgcgat
     3781 tgccggcggc aaaccggagc tgctgcaacc acggatcata cctgtcgcgt gaacaagacg
     3841 aggagacaca tgattccagc acccgcgaat ccgcgggact taggttgcac gaacgatcga
     3901 tgctccaata cagcgaatgc ggatggacac ctgccagtcc gtagttttgg tggcgctcga
     3961 gaggaggtgt cgttatgaaa gctgagatga aactcatatc tgtcaatgtt ggattgccgc
     4021 gggtcgtcac actcaacggc gatccggtct cgactggaat tttcaaagag ccggtcgcag
     4081 gccgggttat gcttcgaacg ctgaacctcg acggtgaccg tcaggctgat ctttcggttc
     4141 atggcggacc cagcaaagcc gtctacgttt atccctccga acactacgat tattggaagc
     4201 aggaattacc cggagtgaaa ctaccctggg gaatgttcgg tgaaaacttc acgagcgtgg
     4261 gactttttga atccgaggta aacataggtg acaggttcag cgtaggttcc gcggtcgtca
     4321 tggtgaccga gccgcggatg ccgtgttaca agctc
//
LOCUS       DASUTG010000426        10702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1992108, whole genome shotgun sequence.
ACCESSION   DASUTG010000426 DASUTG010000000
VERSION     DASUTG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992108"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="sucC"
                     /locus_tag="VF089_09750"
     CDS             <1..603
                     /gene="sucC"
                     /locus_tag="VF089_09750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757829.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEX6174287.1"
                     /translation="DDADCSIAEINPLIITKEGQVMALDAKMNFDSNALYRHKDIVAL
                     RDLNEEDPREIEASKFDLSYISLDGNIACMVNGAGLAMATMDIIKLCGGEPANFLDVG
                     GGASKEKVAQAFKILLADPRVRGVLINIFGGIMRCDVLAQGIVDAAREMNIQVPLVVR
                     MEGTNVTEGKKILADSGLAIIPAETMAQAAEKIVAAVDKR"
     gene            620..1498
                     /gene="sucD"
                     /locus_tag="VF089_09755"
     CDS             620..1498
                     /gene="sucD"
                     /locus_tag="VF089_09755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX6174288.1"
                     /translation="MAILVNRHTRVLTQGITGATGQFHTTACKEYGTQMVGGVTPGKG
                     GTSYDGIPIFDTVAQAVAETAANATVIYVPPPFAADAILEAADADIPLIICITEGVPV
                     RDMVRVKRYLSGKSSQLLGPNCPGVITPGECKIGIMPGHIHRQGSIGVVSRSGTLTYE
                     AVDQLTRLGLGQSTCVGIGGDPVHGFEFVDVLDLFNRDPATEAVVMIGEIGGSAEEEA
                     AGWIKANMKKPVIGFIAGQTAPPGKRMGHAGAIISGGKGTAREKIEAMKTAGIKMSDS
                     PATIGQTMQEALKGHE"
     gene            1491..1919
                     /gene="ndk"
                     /locus_tag="VF089_09760"
     CDS             1491..1919
                     /gene="ndk"
                     /locus_tag="VF089_09760"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6174289.1"
                     /translation="MSEVTLSIIKPDAVQRNLIGEILRRFESAGLKVVAGKLLRLSPE
                     QAKAFYAVHKERPFFASLCAYMSSGPIFVSVLEGENAIAKNREIMGATDPAKAVPGTI
                     RKDCGTDVEANAVHGSDGSETASAEIAFFFKPEEVFRSSN"
     gene            1950..3008
                     /gene="rlmN"
                     /locus_tag="VF089_09765"
     CDS             1950..3008
                     /gene="rlmN"
                     /locus_tag="VF089_09765"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX6174290.1"
                     /translation="MKPNIKDLSFNEFEAYLLERKQPAYRARQVWQWLYQKRAASFAE
                     MTNLSAVFREQLAADFRISRLAVERRADSADGTVKFLFRLADDRNIESVLIPEAKRLT
                     LCISTQVGCGFGCGFCATALLGLKRNLQASEIVDQILEASRTLTEDRRITNVVLMGMG
                     EPLANYAQTIKALGVMTNNSWGIGISPRRITLSTVGLVPQIRKLMDETNVSLAISLHA
                     ATDELRSQLMPVNRKYSLRQLMDCCRALPIPRRKRITFEYVLLRGVNDSAADAHSLVQ
                     LLRGVRCKINLIPFNPHQGSQYQRPENESVEGFQHILSFHGLQVNVRRPRGDDIAAAC
                     GQLQGETIKERERAVGHC"
     gene            3020..3880
                     /gene="mtnP"
                     /locus_tag="VF089_09770"
     CDS             3020..3880
                     /gene="mtnP"
                     /locus_tag="VF089_09770"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX6174291.1"
                     /translation="MSKQRVGVIGGSGLYQIEGLHHVKEIDLSTPFGKPSDKFIQGSL
                     GETELLFLPRHGRGHRWLPTEINFRANIFGMKKLGVERIISVSAVGSLQEKIAPGHVV
                     IPDQFIDRTTQRASTFFGKGIVAHVSLADPFCKELSAELAKAATTAGATIHPSGTYLC
                     MEGPQFSTRAESHLYRGWGADVIGMTNLQEAKLAREAEICFATLALATDYDCWNESAG
                     DVEIDHVLAVLQQNVDLAKKTIRQVIENQSRARSCSCASALKTAIITDKSKIPKKVRR
                     DLRPIIGKYL"
     gene            3877..4782
                     /locus_tag="VF089_09775"
     CDS             3877..4782
                     /locus_tag="VF089_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6174292.1"
                     /translation="MSILVVGTVAFDSIETPFGSAERILGGSASYFALGASFFAPVRI
                     VGVIGQDFPQEYLDLFSQRQIDLSGIKKASGDTFHWRGRYHEDINVRDTLELHLNVLS
                     GFVPDLPEQYRDAEYVFLGNIDPLMQLEVLNQIRRMRLVVCDTMDHWIRESLDGLRQV
                     LKRIEMLVINDSEARLLSGQGNIVRAAREILKMGPEVVLVKRGEYGVLQFSDSSIFAT
                     PAYPLEEVFDPTGAGDSFAGGFLGQLARSGDLTQQGVRRAIVYGSVVASFTVEDFGVK
                     RLASIALQDIEERYRSFAQLTDFHT"
     gene            4787..5740
                     /locus_tag="VF089_09780"
     CDS             4787..5740
                     /locus_tag="VF089_09780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6174293.1"
                     /translation="MDLSVVIPVFNEEDNLKPLISEIEAALNSLGKRYEIVVIDDGSQ
                     DNTFAVLAEIYRVNPHLRVVKLKRNFGQTAALAAGLAHATGEIVVMLDGDGQNDPADI
                     PALMSKLEQGNDIVCGWRYHRRDPFLSRRLPSMIANSVISWTTQVKLHDYGCTLKAMR
                     KEIAKNLKLYGEMHRFIPAIAYERGAKIAELKVNHRPRLRGKSKYGITRTVRVILDLM
                     TVKFLMSYSTRPSHIFGIIGIGSGILGFILGIYLSVQKLMYNISIGTRPLLLLAVMLI
                     FVGFQFVTMGLLGEMLSRTYHESQDKAIFVVAEVLGERSCV"
     gene            5737..7041
                     /locus_tag="VF089_09785"
     CDS             5737..7041
                     /locus_tag="VF089_09785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6174294.1"
                     /translation="MKLAVIGTGYVGLVAGTCFSETGNEVVCVDIDEAKIATLQQGRI
                     PIYEPGLEEMVRRNAEEQRLMFTTDLNSAVRNSSIIFIAVGTPQGLNGHANLEYVRNT
                     SKSIAKAMDGFKIIVTKSTVPVGTADQIRQWISEETSQPFAVLSNPEFMKEGAAVEDF
                     MKPDRVVLGGDDAEALDILKELYEPFLRTGNQILVMDSRSAEMSKYAANAMLATKISF
                     INEVSRLCEYMGADINDVRRAIALDRRIGQHFIFPGVGYGGSCFPKDIRAIISMGGDQ
                     EEMALMKAVEQVNERQKRVLVEKVKRRFGAKLAGKTFAVWGLAFKPRTDDMRDAPAIV
                     VIEALLQSGARIQAFDPEATAEARKIFGDRIVYAARNYDALDGASALLVLTEWNEFRR
                     PDFERIKELLKEPIIFDGRNIYDPGELKRLGFDYYSIGRKNG"
     gene            7034..7981
                     /locus_tag="VF089_09790"
     CDS             7034..7981
                     /locus_tag="VF089_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX6174295.1"
                     /translation="MARYLITGGAGFIGSHLCETFLGQGHDVICMDNYSTGAQQNIAG
                     FAANRRFTFMDHNVSRYIEVQQPLDFVLHFASPASPVDYLELPIPTLKVGSLGTHNAL
                     GLAKAKNAVFLLASTSEVYGDPLVRPQHEEYWGNVNPVGPRGVYDEAKRFAEAMTMAY
                     HRYHGLDTRIVRIFNTYGPRMRMQDGRVVPNFILQALKGEPLTVYGQGEQTRSFQYVD
                     DLVAGIGRLLESGEHYPVNIGNPHEMTVLQFAKKILELTGSKSEIAYRPLPQDDPQVR
                     QPDITKARKLLNWEPKVDLDEGLIKTIDYFRGRLEGQQS"
     gene            7978..8910
                     /locus_tag="VF089_09795"
     CDS             7978..8910
                     /locus_tag="VF089_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6174296.1"
                     /translation="MKIVVTGGAGFIGSHIVDAYLQQGHEVHVIDDFSTGRKLNLNPS
                     AVLHQSNVADHEAAKLILEIKPDVLNHHAAQMDVRHSVEDPLFDAGVNILGFLNLLEA
                     CKKAGTARVIFASSGGAIYGDEEPIPADEDHPKKPMSPYGVSKMTGELYLAYYYMAFG
                     MRYIALRYANVYGPRQSTKGEAGVVAIFISQLLAEKRPTINGDGKQTRDYVFVGDVVR
                     ANVAALETGYIGGINIGTGRETDVVTLCRMLQQRAHSKVDAVHGPAKTGEQVRSCLDI
                     ALAGRVLDWRPEIRLEEGLTQTLAYYREKEPHES"
     gene            8900..10693
                     /gene="lepA"
                     /locus_tag="VF089_09800"
     CDS             8900..10693
                     /gene="lepA"
                     /locus_tag="VF089_09800"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX6174297.1"
                     /translation="MSLEHIRNFSIIAHIDHGKSTLADRLLEFTGAVSAREKTDQILD
                     SMELERERGITIKASPVCLRYRAKDGKDYKLNLIDTPGHVDFTYEVSRSLAACEGALL
                     IVDAAQGVEAQTVANVHMALDHNLEILPVINKIDLPSADPERVRKDIEDVIGLDASNA
                     ILASAKEGVGTEEILEGIVQRFPHPKGSVDDPLRALIIDSWFDPYHGAVVMMRVFDGT
                     VAKGSRIRMMFSGRAFEVSRLGVFAPAPIEVEELAAGEVGFLMAAIKEVAETRVGDTI
                     TLDNRPAREPLPGFKPMKPMVFSGLYPTDANQYQGLREALDKLRLNDSSFTFEPETSQ
                     ALGFGFRCGFLGLLHMDIIRERLEREFSLSLITTAATVVYRVIKTSGEALQIDNPVRL
                     PPEGDIEQIEEPIIQATIHLPQEFLGGVLGLCEEKRGTQKEIRYLGPKRVMIVYELPL
                     NEIVVDFYDRLKSVSRGYASMEYELAGYRVADLVKLDVRINGDVVDAMSMIVHRDKAY
                     YRGRDLVQKMRELIPRQMFEVVIQAAIGSRIISREAVKALRKNVTAKCYGGDITRKRK
                     LLERQKEGKKRMKQVGKVEIPQEAFLAALKV"
BASE COUNT         2497 a         2786 c         3067 g         2352 t
ORIGIN      
        1 gacgatgccg attgttcgat cgcggagatc aaccctctga tcatcaccaa agagggtcaa
       61 gtcatggctc tggacgctaa gatgaacttc gacagcaatg cgctgtatcg ccataaagat
      121 atcgtcgccc tgcgtgacct gaacgaagaa gatccgcgtg aaatcgaagc aagcaagttt
      181 gacctgtcct atatctcttt ggacggtaat atcgcttgca tggttaacgg cgccggtttg
      241 gcgatggcga ccatggatat catcaagctg tgcggcggtg agccggcaaa ttttttggat
      301 gtgggcggag gcgcttccaa agaaaaagtt gcacaggcgt tcaaaattct cctcgcggat
      361 ccgcgagtgc gcggtgttct gatcaatatt ttcggcggca tcatgcgctg cgacgtgttg
      421 gcccagggta tcgtcgatgc ggctcgcgag atgaacattc aagtgccact ggtggtgcgg
      481 atggagggca ccaacgtgac tgagggcaag aagattctcg ccgattcggg tttggcgatc
      541 atcccggcgg aaacgatggc gcaagcggca gaaaagatcg tcgccgcggt cgacaagaga
      601 taatcttagg gaatcttaaa tggctattct cgtgaacagg cacacccgag tactgaccca
      661 gggaatcacc ggcgccaccg gacaatttca taccacagca tgcaaagaat acggcactca
      721 aatggtcggc ggcgtaaccc ccggcaaagg gggcaccagc tacgacggca taccgatctt
      781 cgacacagtt gcccaagccg tagccgagac agcagcgaat gccacggtca tatatgtgcc
      841 gccaccgttt gccgccgatg cgattctaga agccgcagac gccgacattc cgctgatcat
      901 ctgtatcacc gaaggcgtgc cggtgcgcga catggtaaga gttaagcggt atctgagcgg
      961 taagagctcg cagctattag gacccaattg tcccggggtg attacacccg gtgagtgtaa
     1021 gatcggcatc atgcccgggc atatccatcg tcagggaagc atcggtgtcg tttcgcgcag
     1081 cggcaccctg acttatgaag ccgtcgatca gttgactcgt ctcggtctgg gacaatcgac
     1141 ctgcgtggga atcggcggtg accccgtgca cggctttgaa ttcgtcgatg tgctcgatct
     1201 gttcaatcgc gatccggcca ccgaagccgt cgtcatgatc ggcgagatcg gcggcagtgc
     1261 ggaagaggaa gcggccgggt ggatcaaagc caacatgaaa aaaccagtga tcggctttat
     1321 cgccggtcag actgcgccgc cgggtaagag aatgggacac gccggcgcga ttatttcggg
     1381 aggtaaaggt acagcgcgtg aaaagatcga ggcgatgaaa acggcaggga tcaaaatgtc
     1441 ggactcgccg gcaaccatcg gccaaacaat gcaggaggct ctgaaaggtc atgagtgaag
     1501 taacgctgtc gattatcaaa ccggatgcgg tgcagcgaaa tctcatcggc gagattctcc
     1561 ggcgctttga atcggccggg ttgaaagtgg ttgccgggaa gttgctccgg ctctcgccag
     1621 agcaagcgaa ggccttctac gcggtgcaca aggaacgccc cttttttgca agcctgtgcg
     1681 cttacatgtc ttccggtccc atttttgtct cggtgctgga aggcgagaat gcaatcgcca
     1741 aaaaccggga gatcatgggc gccaccgatc ctgctaaagc cgtccccgga acgattcgga
     1801 aagattgtgg taccgacgta gaagccaacg cagtgcacgg ttcagacggt tcggaaacag
     1861 cctccgctga gatcgctttc tttttcaaac ccgaagaggt ctttcgatct tctaactaag
     1921 ctttctgccc attcacaagc tgcccggtga tgaagccaaa catcaaagat ttgagcttca
     1981 acgagtttga agcctatctg ctcgagcgca agcagccggc ttatcgcgcg cgccaggttt
     2041 ggcagtggtt gtaccagaaa cgcgctgcat cgttcgccga gatgaccaat ctgtccgcag
     2101 tcttccgcga gcagctagcc gcagatttta gaattagccg gctcgccgtt gagcgcaggg
     2161 cggattccgc agatggcacg gtgaagtttc tgttccggtt ggccgatgac cgcaatattg
     2221 agagtgtctt aattccggaa gcgaagcgac tgaccctttg tatttcaacc caggtcggct
     2281 gcggctttgg ttgcgggttt tgtgccactg ctctcctggg cctgaagcga aatcttcagg
     2341 ccagcgaaat cgtcgatcag atcctcgaag cgagccggac tctaacggaa gaccggcgga
     2401 ttactaatgt agtattgatg ggcatgggag aaccgctggc gaattacgcg caaacgatca
     2461 aggctctggg tgtgatgacc aacaacagct ggggtatcgg catttcgccg cgacgcatca
     2521 ccttatcgac ggtcggactg gtgccgcaga tacgcaagtt gatggatgaa accaatgtaa
     2581 gcttggccat ttcgctgcac gccgccaccg atgagttgcg cagccagctg atgccggtca
     2641 atcgtaaata ctcactgcgt cagttgatgg attgctgccg ggctctgccg atcccgcggc
     2701 gtaaacgtat cactttcgag tacgtgctct tgcgcggcgt caacgactcg gccgccgacg
     2761 cccatagctt ggtgcaactg ttgcgcggtg tccgctgtaa gataaacttg atcccgttca
     2821 atccgcatca gggcagccag tatcaacgac cggaaaacga gagtgtcgag ggttttcagc
     2881 acatcctttc tttccatggg ctgcaggtca atgttcgccg tcccagaggc gatgacattg
     2941 ccgctgcctg cggccagctg caaggtgaaa caatcaagga gagagagcgc gcggtcggtc
     3001 actgctgaca tcgaacaaca tgagcaaaca acgagtcggc gtgatcggtg gcagcggcct
     3061 ttaccaaatc gaaggcttgc accacgtcaa agaaattgat ctcagcactc ctttcggaaa
     3121 accttcggat aaattcatcc aaggatcact gggagagaca gagttgttgt tcctgccgcg
     3181 tcatggcaga ggacaccgct ggttgccgac ggagataaac ttccgtgcca acatcttcgg
     3241 tatgaagaag ctcggtgtcg agcgaatcat ttcggtgagc gcagtcggta gcctgcagga
     3301 aaagatcgca ccgggtcacg tggttatacc cgatcaattc atcgatcgca cgacacaacg
     3361 cgcgagtacc ttttttggca agggtatagt cgctcacgtc agcttggccg atccgttctg
     3421 taaagaactc tcggccgagt tggccaaggc ggcgaccacg gcgggggcaa cgattcaccc
     3481 aagcggcacc tatctgtgta tggaagggcc gcagttctca actcgcgcgg aatcgcacct
     3541 ttatcgcggt tggggcgctg acgttatagg catgaccaac ctgcaagaag cgaagctggc
     3601 tcgggaagcg gagatctgtt tcgccacctt ggcgctggcc accgattatg attgctggaa
     3661 tgaatccgct ggcgatgtgg aaatcgatca tgtgctggcg gtgttgcagc agaacgtcga
     3721 tttggccaag aagaccatcc gtcaggtgat tgaaaatcaa agccgtgctc gcagctgtag
     3781 ctgcgcctcg gccttgaaaa ccgcgattat taccgacaaa tccaaaatcc ccaagaaagt
     3841 acggcgtgat ctgcgcccga tcattggcaa atatctatga gcatcttagt tgttggtacc
     3901 gtcgctttcg attcaatcga gacgccgttc ggatccgctg agcggatcct cggcggttcc
     3961 gcctcctact ttgcgctggg cgctagcttt ttcgcaccgg tgcggatcgt cggcgtaatt
     4021 ggtcaagatt ttccgcagga atatttggat ttgtttagcc aacgacaaat cgatctaagc
     4081 ggcatcaaga aggcgagcgg cgatactttc cattggcgcg gcagatatca cgaagatatc
     4141 aacgtgcgcg atactctgga gttgcatctc aatgtcctgt ccggtttcgt ccccgacctg
     4201 ccggagcaat accgtgatgc cgagtatgtc ttcctcggca atatcgatcc gttgatgcaa
     4261 ctcgaagtgc tgaatcagat tcgtcgcatg cgcctagtgg tctgtgacac tatggatcac
     4321 tggatacgcg aaagcctcga tggtttgcgc caagtgctaa aacgtattga aatgctggtc
     4381 atcaacgatt ccgaagcacg gctgctaagc ggtcagggca atatcgtccg cgcggcgcgc
     4441 gaaatactta aaatgggacc cgaggtcgta ctcgtcaagc gtggcgaata cggtgtactg
     4501 cagttctccg actcatcgat ttttgccacc ccggcttatc cgttggaaga ggtgttcgat
     4561 cctacgggcg ccggcgattc atttgccgga ggctttctcg gacaactggc tcgttccggg
     4621 gacctgaccc agcagggagt tcgccgcgcg attgtctatg gcagcgtggt ggcatctttt
     4681 acagtggaag atttcggggt gaaacggctg gcgagcattg ctctacaaga tattgaggaa
     4741 cgctatcgaa gctttgcaca gctcaccgat tttcacacct gatctgatgg atctgtcggt
     4801 cgtcattccg gttttcaatg aagaggacaa tctcaagccg ctcatctccg aaatcgaagc
     4861 cgcgctaaac tccctcggca agcgttacga aatagtcgtt atcgacgacg gcagccagga
     4921 caatactttc gcagttcttg cagagatata ccgggtcaat ccgcatcttc gggttgtcaa
     4981 actcaagcga aacttcggac aaaccgcggc attggcggcc ggccttgcgc atgccacggg
     5041 cgagatagtg gtaatgttgg acggggatgg gcaaaatgat cccgcagaca ttccggccct
     5101 gatgtcaaaa ctggagcagg gcaatgatat tgtctgcggc tggaggtatc atcgccgcga
     5161 cccgtttctc agccgccgcc tgccttcgat gatagccaat tcggtgattt cctggacgac
     5221 tcaggtcaaa ctccatgatt acggttgcac gttgaaggcg atgcgcaaag agatcgccaa
     5281 gaacctaaag ctttatggcg aaatgcatcg ctttattccc gccatcgcgt atgaacgcgg
     5341 cgcaaaaatc gccgagctga aagtcaatca ccggccgcga ttgcgcggca agtcgaagta
     5401 cggcattacc cggaccgtgc gcgtcatttt agatctcatg acagtcaaat ttctcatgag
     5461 ctactcgacc cggccgtcgc atatcttcgg aatcatcggc atcggcagcg gtattttggg
     5521 atttattcta ggaatttatc tcagcgttca aaagttgatg tataatattt cgatcggcac
     5581 caggccactt ttgctgctag cggtgatgct gatctttgtc ggttttcaat tcgtcacgat
     5641 gggtctgcta ggggagatgt tgtcccgcac ctatcatgag tcgcaagaca aagcgatatt
     5701 tgttgttgct gaagttcttg gcgagaggag ctgcgtatga aacttgctgt catcggaact
     5761 ggctatgttg gattggtagc gggaacttgc ttttcagaaa ccggcaatga ggttgtttgc
     5821 gtcgacatag acgaagccaa aatcgccacc cttcagcagg ggagaatacc tatttacgaa
     5881 ccggggctgg aagagatggt gcggcgcaac gccgaagagc aacgtctgat gtttaccaca
     5941 gacttaaatt cagcggtgcg aaactcgtcg atcattttca tcgccgtggg cacgccgcaa
     6001 ggtctcaacg gtcacgctaa tttggaatat gtccgcaaca cctccaaaag catcgctaaa
     6061 gcgatggacg gcttcaagat catcgttacc aagagcactg tcccggtcgg caccgcggat
     6121 cagattcggc aatggatatc tgaagaaaca tcccagccat ttgccgtgct gtccaatccc
     6181 gaattcatga aggaaggcgc tgcggtagaa gacttcatga aaccggaccg agtagttctg
     6241 ggcggcgatg acgccgaggc cttggatatt ctcaaagaac tctacgaacc gtttctgcgc
     6301 acgggcaatc agatactggt gatggatagc cgtagtgcgg agatgagcaa gtatgccgcc
     6361 aatgccatgc tcgcgaccaa aatatcgttc atcaacgaag tctcgcgatt atgcgagtac
     6421 atgggcgcgg acatcaatga tgtgcgccgg gccatcgcat tggaccggcg tatcggacag
     6481 catttcatct tccccggagt gggctatggc ggctcctgct ttcccaaaga tatccgggct
     6541 atcatcagca tgggcggcga ccaagaagag atggcgctga tgaaggcagt ggaacaagtc
     6601 aacgagcggc aaaagagagt tctggtggaa aaggtcaagc gccgtttcgg cgcgaagcta
     6661 gcgggtaaga catttgcggt ctgggggctt gcattcaaac cccgcaccga cgacatgcgc
     6721 gacgctccgg ccatcgtcgt catcgaagcg cttttgcaat cgggcgctcg gattcaggcg
     6781 tttgatcctg aggcaaccgc agaggcgaga aagatcttcg gtgatcggat cgtctatgcg
     6841 gcgcgaaatt acgatgctct ggacggcgcc agcgcgctgc tggttctcac cgaatggaac
     6901 gagtttcgcc ggccggattt cgagcgcatc aaggagttgc ttaaagagcc gatcatcttt
     6961 gatggacgca acatctatga tcctggggaa ctaaaaagac tcggctttga ttactattcg
     7021 atagggcgga aaaatggcta gatacctgat caccggtggc gccgggttta ttggctctca
     7081 tctctgtgag acgtttctcg gtcaaggaca cgacgtgatc tgcatggaca actattcgac
     7141 gggagcgcag cagaatatcg ccggcttcgc cgccaaccgg cgtttcacct tcatggatca
     7201 taacgtaagc cggtacatcg aagttcagca gccgttggat tttgttcttc attttgcgtc
     7261 gcccgcaagt ccggtggatt atctggaact gcctataccc actctgaagg tgggctctct
     7321 cggcactcat aatgccttgg gcttagctaa ggcaaaaaat gccgtgtttt tactggcttc
     7381 gacttcggag gtttatggtg atccgctggt tcggccgcag catgaagaat actggggcaa
     7441 cgttaatccg gtgggtcctc gcggggtgta cgatgaagcg aaacgttttg ccgaagccat
     7501 gaccatggcg taccaccgct accatggact cgacacgcgc atcgtgcgga tctttaacac
     7561 ctacggaccg cgcatgcgca tgcaggacgg ccgggtcgtg ccgaatttta ttctccaagc
     7621 cctcaaagga gagccgctta ccgtttacgg ccagggcgag caaacccgaa gctttcaata
     7681 tgtcgatgat ttggtcgccg ggatcggccg cttgcttgaa tccggcgagc actacccggt
     7741 gaatatcggc aatccgcatg agatgacggt cttacagttc gccaagaaaa ttctcgaact
     7801 cacgggatct aaaagcgaga tagcttaccg gccattgccg caagatgatc cgcaggtgcg
     7861 gcagcctgat attaccaaag ccagaaaact gctgaactgg gagcccaagg tggacttgga
     7921 cgagggattg ataaaaacca tcgactactt tcgcgggcga ctcgaagggc aacaatcatg
     7981 aagatcgttg taaccggcgg cgcgggtttt atcggatccc acatagtcga tgcctacctg
     8041 cagcaaggtc acgaggttca tgtcattgat gatttttcca cggggcgtaa gctcaatcta
     8101 aacccaagcg cggtgctgca ccaatccaat gtcgctgatc acgaggccgc caagctaatc
     8161 ctagagatca aaccggatgt gctaaatcat catgcagcac agatggatgt gcgtcattcg
     8221 gtggaagatc ctctgtttga tgccggcgtc aatattctcg gctttctcaa tttactcgag
     8281 gcgtgcaaaa aagccggtac cgctcgggtc atttttgctt cctccggcgg cgccatttac
     8341 ggcgatgagg agccgatccc ggctgatgaa gatcatccga agaagccgat gagcccttat
     8401 ggtgtgagca agatgaccgg cgaattatat ctggcatact actatatggc ttttgggatg
     8461 cgttacatcg cgttgcgtta tgcgaatgtt tacgggccgc gtcagtcaac caaaggcgaa
     8521 gccggcgtgg tggcaatttt tatttcacag ttgctcgcag aaaagcgtcc caccatcaat
     8581 ggcgatggta agcagacgcg ggattatgtt tttgtcggtg acgtcgttag ggccaatgtc
     8641 gccgctctgg aaaccggcta tatcgggggc atcaacattg gcaccggacg tgagaccgat
     8701 gtcgtaaccc tgtgccggat gttgcagcag cgggcccaca gcaaggttga cgcggtccat
     8761 gggccggcca aaaccggcga gcaggtgcgc agctgcctcg acattgcctt ggccggccgt
     8821 gtcctggatt ggcggcccga gatccgcctt gaggaagggc ttacgcagac cctagcttat
     8881 taccgagaaa aagagccgca tgagtcttga gcatatacga aatttctcaa tcatcgccca
     8941 tatcgatcat ggtaaatcga ctttggccga tcgcctgctt gaattcaccg gcgcggtgag
     9001 cgccagggag aagaccgatc agatactaga cagtatggag ttggagcggg agcgcgggat
     9061 aacgatcaag gcgagtcccg tttgcctgcg ttaccgcgct aaggatggca aggactataa
     9121 gcttaacctc atcgacaccc cgggccacgt cgatttcact tacgaggtat cgcgcagctt
     9181 ggcggcctgt gaaggggcgt tactgatcgt cgatgccgcg cagggggtcg aggctcaaac
     9241 cgtcgccaac gttcacatgg cgctggacca caatctcgaa attcttcccg tcataaacaa
     9301 gatcgatctt cctagcgctg atcccgaacg ggtgcgcaag gacatcgaag atgtgatcgg
     9361 cctcgacgcc tccaatgcta tcctggccag tgccaaagag ggtgtcggca cggaagaaat
     9421 tctcgaaggc atcgttcagc gttttccgca tcccaaaggg agcgtcgatg atccactgcg
     9481 ggcgttgatt atcgattcat ggttcgatcc ttatcatggg gcggtggtga tgatgcgggt
     9541 cttcgatggc acggtcgcca aaggctcgcg catccgcatg atgtttagtg gccgcgcctt
     9601 tgaagtgagc cgcctcgggg tttttgcccc ggcgcccatc gaagtcgagg aattggccgc
     9661 cggagaagtc ggtttcctga tggcggcgat caaagaagta gccgagaccc gagtcggcga
     9721 cacaatcacg ttggacaatc gcccggcacg agagcccttg cctggattca aaccgatgaa
     9781 gccaatggtg ttcagcggcc tctatccgac cgatgccaat cagtaccagg gtttgcggga
     9841 agcattggat aagttgcggc taaacgattc atcgttcacc ttcgagccgg aaacatctca
     9901 ggccctgggt tttggattcc gctgtggctt tctcggcttg ctccacatgg acattatccg
     9961 ggagcggctc gaacgcgaat tcagtttgag cctaatcacc acggctgcca cggtggtata
    10021 ccgagtgatc aaaacgagcg gcgaagccct gcagatcgat aaccccgtgc gactgccccc
    10081 ggaaggcgac atcgaacaga tcgaagaacc gatcatccaa gcgaccattc atttgcccca
    10141 ggagttcctc ggaggagtgc tcgggctctg tgaagaaaaa cgcggtacgc agaaggaaat
    10201 ccgctatctc ggacccaagc gagtgatgat cgtttacgag ctgcccttga acgagatcgt
    10261 cgttgatttc tacgatcgtt tgaagtcggt cagccggggc tacgcttcca tggagtacga
    10321 gcttgctggc tatcgagtcg cggatctggt taaactagac gtgcgaatta acggagacgt
    10381 ggtggacgcc atgtcgatga tcgtgcatcg ggacaaagcg tactaccgcg gccgcgacct
    10441 tgtgcaaaag atgcgcgagc tcatcccgcg acagatgttc gaagtcgtga tccaagccgc
    10501 cattggcagc cgaatcatat ctcgggaggc ggttaaagca ctgcggaaaa atgtcacggc
    10561 caaatgctac ggcggtgata tcacccggaa aagaaaattg ctggagcggc aaaaggaagg
    10621 aaaaaagcgc atgaaacagg tgggcaaagt agagattccc caggaagcgt tcctcgctgc
    10681 tctgaaggtt tagatcggag tt
//
LOCUS       DASUTG010000427         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1992226, whole genome shotgun sequence.
ACCESSION   DASUTG010000427 DASUTG010000000
VERSION     DASUTG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992226"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..786)
                     /locus_tag="VF089_09805"
     CDS             complement(343..786)
                     /locus_tag="VF089_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6174298.1"
                     /translation="MELVTWTPFESLNKAQSRINGLFDEKFGRSWPNTEAGRAWYPAV
                     DILESKDAYVLRAELPGMNKEDFNLELKEGTLTLTGERKFTKPAEGVQYRKVERVNGK
                     FVRSFTLPETVKHDGIQATYKDGILEIHIPKAEEAKPRQIQISVH"
     gene            complement(906..>2097)
                     /locus_tag="VF089_09810"
     CDS             complement(906..>2097)
                     /locus_tag="VF089_09810"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX6174299.1"
                     /translation="NGKSHHKSAGDLIYDLHFVSQTWQGKNWQHQLQIYRPQSVAPAG
                     MMFLWVTGGSATPGQIAFGMELARKSKAPVAFLYHIPNQPLLEDQLVEDDLIAETFVR
                     YLKTKDENWPLLFPMVKSVVKAMDVLQAFGKGEWGEPASKFIVGGASKRGWTTWLAAA
                     VDPRIGAIAPVVIDTLNMRAQMPRQLKAFGAYSARLQPYTSRGLVPIPESPEGQRLLS
                     MVDPWAYRDRLTLPKLIVNGTNDFYWATDALNLYWNELPANKWVIYVPNAGHDLRRQD
                     RTNSDQRDDLINGLAAFSRHQINGRPMPELSWKHEDRDGKLRLSIAANPQPLAARLWV
                     AQTASKDFRQAKWIEQAADISDGMVVGEVTPTGKGHVAFFGELDYQIDGLKYQLSTQL
                     RVTE"
BASE COUNT          486 a          563 c          540 g          508 t
ORIGIN      
        1 aagcagtggt gagagaatga ttctggcggt gaagttaaga aggaaaatcc ctaccagaaa
       61 aaataccggc cgtaagatcg tcaggaaatt tttcgactcg ccgctcaacg tcgcgcctgg
      121 gtgatttgat tgaactcggg ttcacctgga cttgtaacac taattcattc gtcaacacac
      181 atagaaagga ctaattgagg cggtttgttc gtgatacggg cagttgctta aattgcggag
      241 agatggcgtc gatcaatcct atgaaagaca agtaagccca aaaaagagga ggaagcacgc
      301 tgcttccccc tcctgttaac tactgctgaa cgtgctagaa gattagtgca cgctgatttg
      361 tatttgccgc ggtttggctt cttccgcttt aggaatatga atctcgagta taccgtcctt
      421 ataggtcgcc tgaatcccgt catgctttac ggtttccggc aaggtaaagg atcttacgaa
      481 ctttccatta acccgctcca ctttgcggta ctgaacgcct tcagcgggct tggtaaactt
      541 ccgctcgcct gtgagcgtca atgtgccttc cttaagctcg aggttgaaat cctccttgtt
      601 cattcctggc agctcggctc ggagtacata ggcatctttg ctttccagaa tatcgacagc
      661 gggataccag gctctgccgg cttcggtgtt aggccacgag cggccaaatt tttcatcaaa
      721 caagccattg atccgcgact gcgccttgtt gaggctttca aacggtgtcc atgttaccaa
      781 ttccatatga tgactcctct ctaagcagtg tgatgaggag aaagtaagaa cggcaacctg
      841 gactgtcaac gccttgacaa ggcctggact caaccttaaa ttcttggttc acaaagcaag
      901 atcctttact ccgtcacccg aagttgggtg gagagttggt attttaaccc atcgatctgg
      961 taatcgagct cgccaaaaaa agccacgtgc cctttccccg taggagtgac ttcgccgaca
     1021 accatcccgt ccgagatatc ggccgcttgc tcaatccact tagcttgccg aaaatctttt
     1081 gatgcggttt gcgcaaccca gagccgcgcg gctaacggct gcggattcgc agcgatgctt
     1141 aaccgaagtt tgccgtcgcg atcttcatgc ttccagctta gctcgggcat cggacgaccg
     1201 ttaatttgat ggcgagaaaa agcagctaag ccgttaatta gatcatccct ttgatccgaa
     1261 ttagttcggt cttgtcttct caagtcgtga ccggcgttcg gaacatagat cacccacttg
     1321 ttggcaggca gctcgttcca atagagattg agcgcgtccg tagcccagta gaaatcgttg
     1381 gtgccattga caatcaactt gggcaacgtc agccggtcac ggtaggccca cggatcgacc
     1441 atgctcagca ggcgttgccc ctccggtgac tccggaatgg gcaccaagcc acggctggta
     1501 tacggttgca ggcgggcgct ataggcgccg aaggctttta gttgccgcgg catctgcgca
     1561 cgcatgttga gcgtatcgat gactaccgga gcaatggcgc cgattcgtgg atcgaccgcc
     1621 gcggcaagcc aggtggtcca gcctcgcttc gatgcgccgc cgacaatgaa cttgctcgct
     1681 ggctcgcccc attcgccttt gccgaacgcc tgcaagacat ccatagcttt gacaacactc
     1741 ttcaccatcg gaaacagtag cggccaattt tcatctttgg tcttgagata acgcacgaag
     1801 gtctcggcga tgagatcatc ctcgacgagc tgatcttcca aaagcggctg attgggaatg
     1861 tgatacagga aggccacagg agctttgctc ttgcgcgcca gctccatacc aaacgcaatc
     1921 tggcccggtg ttgccgaacc accggtcacc cagaggaaca tcatcccggc gggggcgacc
     1981 gactgaggcc ggtagatttg caactgatgc tgccagttct tgccctgcca ggtctgggat
     2041 acaaaatgga gatcatagat tagatctccc gccgacttat ggtgcgattt tccgttc
//
LOCUS       DASUTG010000428         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1696493, whole genome shotgun sequence.
ACCESSION   DASUTG010000428 DASUTG010000000
VERSION     DASUTG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696493"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..673
                     /locus_tag="VF089_09815"
     CDS             80..673
                     /locus_tag="VF089_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174300.1"
                     /translation="MTSNSQSAVQRFITSARDLFAQEKDPAELWKKMTPLLESLIGDP
                     SIKEQSRDWPACRQGDERAQNLLFYEDPDYKFVINGLIKSPKSRTQIHDHAHNWTLYG
                     VLDGHESIERYERVDDGAKSDYADIRQTEKVNVGPGKVDLVGPFQIHAEESGEERTVA
                     IIVRAQKAGSFLQGRYDPATKRYWQGYGPEQIPYELK"
     gene            776..2494
                     /locus_tag="VF089_09820"
     CDS             776..2494
                     /locus_tag="VF089_09820"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014537808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX6174301.1"
                     /translation="MADEIKPGRKLRIAVFSGPTATIQNSEPLVTSNKARAKYGLPLR
                     TEPEGTSLRCDVLRPQRLAAPVTVYVEQFSAHPLERDSAELYGPPDGYIDAAGAFHQQ
                     RTSAADKPVYEITLNPDDGLYPLPYMARQANGQPWEMDGTEKDAPAELCRVPFFPDGS
                     RLFEEIDRLGISDEGVGCMLSAKAEFDFYRALPSGGYAKGRSAGERTDIGDGAIAPEI
                     RGTDFFPYRPPYLRQEPPMSALARVTNIVQGALNSGTYSGAIWLEGSPFVEETIYWLN
                     LVIDTTVPIVGNSSQRPHGAIGNDGDRNIVDSVDYITSRIWADESGKDTVGAVAILDE
                     QIFTSRDVQKADARPGGYIATGGHGGIIGRIGHPGPPQFSFKTVKRHTHRSLVNTRHL
                     PDRVQGARIQGSQIGTIAVPIKDGTGNLLPTAIPKVTLIKHARYLPEDTTGTAEQEVD
                     ILARIDKNLRDAPLAGFVAEGSAPFGAMSNSVDAALKRATFSGMPVVKVGRGNAGGFV
                     DAKRDPLSIAGNNLTATKARLLLMACLLKVGCLPPAADAAKPSVTEIEATKDKLKQYQ
                     EIFDTH"
     gene            2599..3672
                     /locus_tag="VF089_09825"
     CDS             2599..3672
                     /locus_tag="VF089_09825"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6174302.1"
                     /translation="MSGKHGHKPFEGHMHDYEAHITAVEEDLEYYRAKRLEPRIIYLI
                     RRGIVTFYDLLKARAALKRANQFFETTKPQGKNLEARVRYLEEWLDEYVKGIALVSSR
                     SVEGMDMVIEDNRKERGDYDDFFRIKKKEHHGTLAERMTNLEQDLKDYQELLEVFVQA
                     LIARKWATRQELEQRWKQLHEERPWAGAVIVAKAWTDPEFKQALLATGREALREMGVH
                     QGKVGKLLVVENTEAVHNVVVCTLCSCYPYDILGDTPWWYKHDIYKERIVQNPRAVLK
                     EMFSLDLPAEQEVRVWDSTSDVRYFVLPKRPAGTDGMSAEELAKLVTIDSLIGAGLAK
                     EASELSEARQAGAVPEIPRVRPD"
     gene            3681..3971
                     /locus_tag="VF089_09830"
     CDS             3681..3971
                     /locus_tag="VF089_09830"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX6174303.1"
                     /translation="MSVSNCKFKIGDVVVVATEMAHRNNPRTPDYIRGKRGVIGLVHG
                     VLENPKDHRGLYDPLYTVRFDLSALSSCSDRDSIWVDVHEEWLSSLRPEDKN"
BASE COUNT          978 a         1030 c         1156 g          849 t
ORIGIN      
        1 taaacgcgcc attctccttg acctggagcg agcggtctga ctaataattg cttgaggtta
       61 ccaggacaca ggagggctta tgacttccaa ttcgcaatct gcagttcaac gtttcatcac
      121 atcggcacgc gaccttttcg ctcaggagaa agatcctgcc gagctctgga aaaagatgac
      181 gccgctgctc gaaagtctga tcggcgatcc ctcgataaaa gaacagtcaa gggattggcc
      241 tgcttgccgg caaggcgatg aacgcgcgca aaatctgctt ttttacgaag atccggacta
      301 caagtttgtc atcaacggct tgatcaagag cccaaagtcg cggacgcaga tccatgatca
      361 tgcgcacaac tggaccttgt acggcgtact cgacggtcat gagtcgattg aacggtacga
      421 gcgggttgat gatggcgcca aatccgacta cgccgatatc cgtcagacgg agaaagtgaa
      481 cgtaggtccg gggaaggtag acctcgtcgg gccttttcag attcatgccg aagagagcgg
      541 cgaagagcgc acggttgcga ttatcgtccg ggcgcaaaaa gccggcagtt tcttacaagg
      601 ccgctatgat ccggcgacga aaagatactg gcaaggttac gggcccgagc agattcccta
      661 cgagctcaag taatttcctt atcggttcgg tattgcggcg atcggcttta aaccgattgc
      721 ggacaagtga ccaaactcct ccaatccaca acgttcacca gcagcggaga cttgcgtggc
      781 agacgaaatc aagcctggta gaaagcttcg tatcgcagtt ttttcaggac ctacggcgac
      841 gatccaaaac agcgagccat tggtaacgag caacaaggca cgcgccaaat acggtctacc
      901 gctgcggacc gaacccgagg gtacttccct tcgttgcgat gttctaaggc cgcaacggct
      961 ggctgctcca gtaactgttt atgtcgaaca attcagtgct cacccgctag aacgggattc
     1021 ggcggaactg tacgggccgc cggacggcta tatagatgcc gccggagctt ttcaccagca
     1081 gcgcacgagt gcagcggaca aaccggttta cgagatcacg ctcaatcccg acgatgggct
     1141 ttatcctttg ccctatatgg cgcgacaagc caacggccag ccctgggaaa tggacggaac
     1201 ggagaaagat gcgccggcag aactctgccg ggtgccgttt ttcccggacg gctcccggtt
     1261 gtttgaagaa atagatcgtc tgggcatatc cgatgagggc gtcggttgta tgcttagcgc
     1321 caaagccgaa ttcgatttct atcgcgcgct cccttccggc ggttacgcca aagggcgaag
     1381 cgcgggcgag cgcacggaca taggagatgg cgcgattgcc ccagaaatcc gcggcactga
     1441 tttttttcct tatcggccgc cgtacttgcg gcaagagccg ccgatgtcgg cactagcgcg
     1501 ggtcaccaac atcgttcaag gcgcgctgaa cagcggaaca tattccggtg ccatttggct
     1561 ggagggaagc ccgtttgtcg aggagacgat ttattggctc aatctagtga tcgataccac
     1621 agtgccgatt gtcggaaatt cctcgcagcg gccgcatggc gctatcggca acgacggaga
     1681 tcgcaacatc gttgattcgg tagattacat cacatcgaga atctgggctg atgagtccgg
     1741 aaaagacacg gttggcgccg tagccattct cgatgagcag attttcacct cgcgcgatgt
     1801 gcaaaaagcc gacgcgcgcc ctggtggtta cattgccacc ggaggtcatg gtggcattat
     1861 cgggaggatc ggtcatcccg ggccgccgca gttctcgttt aaaacggtca agcggcatac
     1921 gcacaggtcg ctggtgaata cccggcacct gccggaccgg gttcaaggtg ccagaattca
     1981 gggatcacaa atcggcacca ttgccgtgcc aatcaaggat ggtacaggaa atctgttgcc
     2041 gacagcaatt ccaaaggtca ctctaatcaa gcatgctcgc tatcttcccg aagacactac
     2101 cggcactgcc gagcaagaag tcgatattct cgcgcgaatc gacaagaatc tccgcgatgc
     2161 gccgctggct gggttcgtcg cagagggttc agcgcctttt ggcgcgatga gcaattccgt
     2221 cgatgcggcg ctcaagcgag ctacttttag cggcatgccg gtggttaaag ttgggagagg
     2281 caatgccggc ggctttgtcg atgcgaagcg agacccgtta tccattgcag gtaataatct
     2341 taccgctact aaagcgcggc tgttgttgat ggcctgtctg ctgaaagtgg gatgtttgcc
     2401 gccggcggcg gatgcggcaa agccgagcgt cacggagatc gaggctacaa aagacaagct
     2461 gaaacagtac caggagattt tcgacactca ctgacggctg caatggtcat caggtgcgca
     2521 gccggcgata aaaagccgcc atccttatga atagcacgga gcaagctcgt cgctcgtgag
     2581 ccgtaatgga gaatcaatat gtcaggaaaa catggacaca agcctttcga agggcatatg
     2641 cacgactacg aagctcacat aaccgctgtc gaagaggact tggaatacta tcgggcgaag
     2701 cgtctggaac cgcgcattat ttatctcatc cgtcgtggaa tcgttacctt ttacgatttg
     2761 ctcaaggcgc gcgccgcgct caaacgcgct aaccaattct tcgagacaac aaagcctcaa
     2821 ggaaagaatt tggaagctcg ggtgcgttat ctggaagaat ggctcgacga gtacgttaag
     2881 gggatcgcgc tcgtttcttc acgcagcgtc gaaggcatgg acatggtgat cgaagataac
     2941 cgcaaggagc gcggtgatta cgatgatttt ttcagaatca aaaagaaaga gcatcacggc
     3001 acgttggcag agcgtatgac caatttggag caggatctaa aagactacca ggaactgctc
     3061 gaggttttcg tgcaagccct gatcgcccgg aagtgggcga cgcgacaaga gttggagcag
     3121 cgctggaagc agcttcatga ggagcgaccc tgggccgggg cggtgatcgt cgctaaagcc
     3181 tggacggatc cggagtttaa acaggctttg ctggcgaccg gaagggaagc gctcagggaa
     3241 atgggtgtgc accagggtaa ggtcggcaag ttgctcgttg tggaaaatac cgaggctgtt
     3301 cacaatgtcg tcgtttgcac cttatgctcc tgttatccgt atgacattct tggcgatacc
     3361 ccctggtggt ataagcatga tatctacaaa gaacgaattg tgcagaaccc acgcgcggtg
     3421 ctcaaagaga tgttttcgct ggatcttcct gctgaacaag aggttcgggt gtgggacagc
     3481 acttcggacg ttcgctattt tgttttacca aagcgacctg ccggcaccga cggcatgtcg
     3541 gcagaggagc tggccaagct cgtcaccatc gacagcttga ttggcgctgg gttagcgaag
     3601 gaagcttccg agctaagcga agcccgccag gccggagcag tgccggagat tcccagagtt
     3661 cgacccgatt gatgagacct atgagcgtaa gcaattgtaa atttaagatc ggcgacgtgg
     3721 ttgtcgtggc tacggaaatg gcccatcgga ataatccacg aacgcccgac tatatccgcg
     3781 gcaaacgcgg cgtgatcggc ttagttcacg gtgttttaga aaatcccaag gatcatcgcg
     3841 gtctatacga tccgctttac accgtcaggt tcgatttgag cgcgctctct tcgtgctccg
     3901 atcgtgattc tatctgggtc gatgtgcatg aagaatggtt gagttcgctt cgaccggaag
     3961 acaaaaatta gctgccataa ccaagtcgat tcaaacagga ggtcacgtga ata
//
LOCUS       DASUTG010000429         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1696515, whole genome shotgun sequence.
ACCESSION   DASUTG010000429 DASUTG010000000
VERSION     DASUTG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696515"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VF089_09835"
     CDS             <1..580
                     /locus_tag="VF089_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX6174304.1"
                     /translation="KFLISSSAVGYYGSRGDETVTEETAPGHDYLSRVCVDWEKEASN
                     AETYDVRVVLVRTGIVLDKGKGALTKMVVPFKFFVGGPLGSGNQWMPWIHIDDQIALL
                     LFLLENQNARGPFNATAPNPVTMTEFCKTLGAVLNRPSWASVPGSMLTLLVGEMAEML
                     LNGQRAVPQAAINLGYKFKYPHLLPALESLHL"
     gene            663..1832
                     /locus_tag="VF089_09840"
     CDS             663..1832
                     /locus_tag="VF089_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6174305.1"
                     /translation="MSNPQRVPARLSGVKEEFLKNSSFNLSDEVSAARIILDGPNQDY
                     CETGRQELGTVLPASERMAELLGLDDPSIVQDVLRDALLDPLAESTSTRGKRIRGQLV
                     TLVYRMVSDTPASLLAAKQCGWCADLVELIHTGSLVIDDIEDGSTVRRGRPALHVQFG
                     LPIALNTGNWLYFWPFELLRETELPANKIFYVYERCHRTLLRAHFGQAVDLGARVESL
                     PQHRVAAVCLASLKLKTGALMGFASILGGVIAGASEPVLSILDDFGRELGVSLQMFDD
                     LGNLIGKCEPAKRYEDLRLSRPSWVWACAADSSASKAYEDFRTAVGSLPDAHELELWS
                     EAHNLVERVRACARGYLDGAFRQLKERLDSANVRCSRRAFEELRELGEEIAIAYG"
     gene            1825..3360
                     /locus_tag="VF089_09845"
     CDS             1825..3360
                     /locus_tag="VF089_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013791490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX6174306.1"
                     /translation="MARVSAAVIGSGFGGLALAIRLQAAGCRTVIFEKRDQAGGRAYV
                     YRDRGFTFDAGPTVITAPDCLTELFTLAGRSIADYVTLLPVHPFYRLFWEDGFCFDYS
                     ADLADVERQIASKSPRDLRGYKSFLKYSERVFEEGYVKLAHVPFLDWSSMVKVAPQLF
                     RLKAYRSVHSIVAKFIQDPHLRQLFSFHPLLVGGNPFTTTSIYTLIHCLERKWGVFFP
                     KGGTGALVQGMLKLFEQLGGRIRLQSEVSEILTRGNRVLGIATQTGERHEFDLVASNA
                     DVVHTYDRLLGREPKVRRMRKKLAGMCYSMSLFLIYFGTRRQYPNLQHHNIIFGPRYR
                     ELLKDIFGRGRLADDFSLYLHAPTRSDPSLAPPGCEAFYVLSPVPHLGKLPVDWSVEG
                     PRYAERILDYLERHYIPNLRNEIVTQRIFTPQDFQSELNAHLGSAFSLEPRLMQSAYF
                     RVQNRDAHLTGLYLVGAGTHPGAGIPGVVNSAKATAGLILDDIRKAVVMPSTDQVQRL
                     SYA"
     gene            3353..4306
                     /locus_tag="VF089_09850"
     CDS             3353..4306
                     /locus_tag="VF089_09850"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX6174307.1"
                     /translation="MPEAMSPRVKAALDECRWMITQGSKSFSLAARLFDPDTRDAAFF
                     LYGWCRYCDDQVDRTGLEESREQLEQRLAKLSEATRSVFSGAAQDQAVFIAMQYIVQR
                     YAIPAHYALELIEGMAMDARGTRYQTFNDLSLYCYRVAGTVGLMMSHVMGLRSERALK
                     HAADLGIAMQLTNIARDITEDASMGRIYLPLEWLEEAGIAPEEIAAVKNRSKLALITK
                     WLLQEAQRYYLSGDAGLWHLSFRSACAVAAARHVYAEIGSLLLHRGPSAWDRRSYVTG
                     PRKLWVILGGVLHVIASMPARLIRPWSAAPIHRVWRHSELR"
     gene            4346..5191
                     /locus_tag="VF089_09855"
     CDS             4346..5191
                     /locus_tag="VF089_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX6174308.1"
                     /translation="MHFIPWLCLSFQGAIVAASLLGYGIFTSRPELLTQLDPGARFFT
                     WAFYGFAVGNMLFGGLAVVAEAVLRDRRRALWAFALVYLVSLVGELLGTTYGIPFGAY
                     SYTALLGVKWFDRVPLLIPLSWFTVSWACWVIARQRSGGVSAILLGTALLVAWDLLLD
                     PAMSKVTSYWIWGEKGSYYGMPWMNLLGWCVTGLVLFIILSKIAPRPHSQVRFAISVY
                     IVNFALPLGFCALNQYWLAVCAGLGAVATAFFVFGRTGSALAARDLRDHRPSGRAGAL
                     SSQRE"
BASE COUNT         1076 a         1327 c         1541 g         1375 t
ORIGIN      
        1 gaaatttctc atcagctcct ctgcggtagg ttattacggg tctcgtggtg atgagaccgt
       61 cacagaagaa actgctccgg gccatgatta cttgtcgcgg gtttgtgttg attgggaaaa
      121 agaagccagc aacgctgaaa cgtatgatgt acgggtggtg ttagtaagaa ccggcatcgt
      181 tttggacaaa ggtaagggag cgttgactaa aatggtcgtt ccatttaagt tttttgtcgg
      241 cggtcccctc ggatcaggta atcaatggat gccgtggatt catatcgacg accagatcgc
      301 cttgctgttg tttctcctgg aaaaccagaa tgcccgcggc cctttcaacg ccacggctcc
      361 gaatccggta accatgacgg aattttgcaa aacactgggg gcagtgttga accggccatc
      421 atgggcgagc gtcccgggga gtatgttgac tctgctcgtg ggcgagatgg ccgagatgct
      481 gttgaacggc cagcgtgctg tgccgcaagc cgccataaat ctgggttata agtttaagta
      541 tccccatctg ttgccggctt tggagtcgtt gcatctttaa taagcgatga gcccagccaa
      601 ccattcaggg gacagaagct tctgcactca ggctttgtta agtggcgatg agcacgctgc
      661 ttatgagcaa cccgcaacgg gtgcctgcgc ggctctccgg cgttaaggag gaattcttga
      721 agaactcaag cttcaacttg tcggacgagg tatcggctgc ccggattatt ttggatgggc
      781 cgaatcagga ttattgtgaa acaggtcgac aagagctcgg aaccgtgctt cccgcgagtg
      841 aacgcatggc tgaactactt ggattggatg acccatctat cgttcaggat gttctgcgtg
      901 atgctttgct tgaccccctt gccgaatcga cttctacccg tggtaaacgc atacgtggac
      961 agctggtgac cttagtctac cgtatggtca gtgacactcc ggcgtcgctt cttgccgcca
     1021 agcaatgcgg ctggtgtgcc gatctagtcg aattgattca cacaggatcc ctggtgatcg
     1081 acgatatcga ggacggcagc accgtgcgtc gggggcgccc cgcgttgcat gttcaatttg
     1141 gtctccccat cgcgctcaac accggcaact ggctctactt ctggcctttc gagttgcttc
     1201 gagagacgga actgccggcc aataagattt tctacgttta tgagcgttgt catcgaacgc
     1261 tgttgcgtgc tcactttggg caagcggttg atttgggagc ccgggtcgag tcactgcccc
     1321 agcaccgcgt tgccgcagtt tgcttagcct cattgaaact caagaccggc gcattgatgg
     1381 gtttcgcgag cattttgggt ggggtcatcg caggagcatc ggaaccggta ttgtcaattc
     1441 tcgacgattt cggccgggag ctcggcgttt cacttcagat gttcgatgat ttgggtaatc
     1501 tcatcggcaa atgcgaacct gccaagcggt atgaagatct tagattatcc cgaccatcat
     1561 gggtctgggc atgcgccgca gacagtagcg cctccaaagc gtatgaagac ttccgtactg
     1621 ctgtcggcag tttaccggat gcgcatgaac tggagttatg gtctgaggcg cacaaccttg
     1681 tagaacgggt gcgtgcctgt gctcggggct atttggatgg cgcgtttcga cagctgaaag
     1741 agcggctaga cagcgcgaac gtacgctgtt cgaggcgggc ttttgaggag ctacgagaat
     1801 tgggcgagga gattgcaatc gcgtatggct agagtctccg ccgccgttat cggaagtggt
     1861 ttcggcgggc tcgccttagc catccggtta caagcagccg gttgtcgcac ggtaattttc
     1921 gaaaagcgcg accaggcggg aggacgggcg tatgtttatc gcgaccgcgg ctttacgttc
     1981 gatgccgggc cgacggtgat tacagctcct gattgtttga cagagctttt tacattggca
     2041 ggaagatcaa tcgccgatta tgtcacactg ctgccggttc atccttttta tcgtctattt
     2101 tgggaggacg gtttttgctt cgattactcg gccgatctgg ccgacgtcga gcggcagatc
     2161 gccagtaaat caccgaggga tctacgcggc tacaagagct ttctaaaata ctccgaaaga
     2221 gttttcgaag aaggctacgt gaagctggcg catgtaccct ttttggattg gtcgagtatg
     2281 gtgaaggtag cgccgcaact ctttcggctc aaagcttatc gatcggtgca tagtatcgtc
     2341 gctaagttta ttcaagatcc gcatcttcga cagcttttta gcttccatcc attactcgtc
     2401 ggcggcaatc cttttaccac cacctcgatt tatacgttaa ttcattgttt ggagcggaaa
     2461 tggggcgtgt ttttcccaaa aggcggcacc ggcgcactgg tgcagggaat gctgaagctg
     2521 tttgagcaat taggtgggag aattcgattg cagtcggaag tgtccgaaat tctaacccgc
     2581 ggcaatcgtg ttctggggat cgcgacccaa accggtgaga gacacgagtt tgatttggtg
     2641 gcgagtaatg ccgatgtcgt tcatacctac gatcggcttc taggtcggga gccgaaagtc
     2701 cggcgcatgc gcaaaaaatt ggccgggatg tgttacagca tgtcgttgtt tctgatttat
     2761 ttcggtacgc gccgccagta tccaaacctg caacaccaca atatcatctt tggaccgcgt
     2821 tatcgtgagc ttctgaaaga cattttcggc cgcggtcggt tggcagatga cttctcgctt
     2881 tatctgcatg cgccgacacg atccgatcct tcattggcgc caccaggttg tgaagccttc
     2941 tatgtgttat cacccgtgcc tcatctgggt aagctgcctg tggactggag cgtggaaggg
     3001 ccgcgttatg cggagcgaat ccttgattat ctcgagcggc attacattcc aaacctgcgc
     3061 aacgagattg ttacgcagcg gattttcacg ccgcaggatt ttcaatcgga gctcaacgcc
     3121 catctgggat cggcgttttc gttggaaccc cgcttaatgc agagtgcgta ttttcgggtg
     3181 cagaatcgtg acgcacatct caccggcttg tatcttgtcg gggccggaac ccaccccggt
     3241 gcaggaattc ccggcgtcgt aaactcggcc aaggcaaccg cggggcttat tctcgatgac
     3301 attagaaaag ccgtggtgat gcccagtact gaccaggtgc agagattatc ctatgcctga
     3361 agcgatgtcg ccgagagtta aagcggcctt ggacgaatgc cgctggatga tcacccaagg
     3421 ttcgaaaagt ttttcgctcg cggcgcggct ttttgatccc gacactaggg atgcggcatt
     3481 ttttctttat ggctggtgcc gttactgtga tgatcaggtg gaccggacgg gattggaaga
     3541 gagccgtgaa caacttgaac agcggctagc caagctctcg gaggcgactc gctcggtctt
     3601 ttccggtgct gcacaggatc aagcggtatt tatcgccatg cagtacatcg tccaacgtta
     3661 tgcgatcccg gcgcattatg cgctggaact aatcgaagga atggctatgg atgctcgtgg
     3721 cacccgttac cagaccttta acgatctttc gctttattgt taccgggtgg cgggaactgt
     3781 gggattaatg atgtctcacg tcatgggact ccgcagcgag cgcgcgctga agcacgcagc
     3841 agacctcggc atcgcgatgc agcttactaa catcgcccgg gatatcaccg aagacgcatc
     3901 gatgggtaga atatatctgc ctttggagtg gttggaagaa gcaggtatag caccggaaga
     3961 gatcgccgcg gtaaagaatc gttcaaagct ggcgctaatc acaaaatggc tccttcagga
     4021 agcgcaacgg tactatcttt ccggagatgc cggcttgtgg cacttgtcat ttcgttccgc
     4081 gtgcgctgta gcggcggcgc ggcatgttta tgccgaaatc ggctcgctgc tcttgcatag
     4141 agggccttcg gcttgggacc ggcggagcta tgttacaggg ccccgtaaac tttgggtcat
     4201 acttggcggg gtgctccacg tcatcgcttc tatgccggcg cgattgatcc ggccatggtc
     4261 tgccgcgcca attcatagag tgtggcggca ttcagagctc aggtgatctg gaaccgaatt
     4321 taatcgtagc agggagattg tcccgatgca ctttatcccg tggctgtgtc tgtcattcca
     4381 gggcgccatc gttgctgcta gtttgttggg ctacggtatc ttcacttctc gccctgagct
     4441 tttgacacaa ctggacccag gcgctcggtt ttttacttgg gctttttacg gattcgcagt
     4501 gggcaatatg ctgttcggcg gccttgctgt cgtcgcagaa gcggtgttgc gcgatcgaag
     4561 gcgcgctctg tgggcctttg ccttggtcta tctagtcagt cttgtcggcg aattgcttgg
     4621 gacgacctat ggaatcccgt tcggcgccta ttcctatacc gcgctgctcg gcgttaagtg
     4681 gttcgatcgc gtaccgctct tgatcccatt gagctggttt accgtgagtt gggcctgttg
     4741 ggtcattgcg cggcagcgta gcggcggagt ttcggccatc cttctcggca cggctctcct
     4801 cgtggcctgg gatcttttgc tcgacccggc gatgagtaaa gttaccagtt actggatttg
     4861 gggcgagaag ggcagctact atgggatgcc ctggatgaat ctgctaggtt ggtgtgtcac
     4921 cggtttggtg ctattcatta tactttccaa gatcgccccg agaccgcata gccaagtgag
     4981 gtttgcaatt tcggtataca tcgtcaattt cgctttgccg ctcggatttt gtgcgttgaa
     5041 tcagtattgg ttggcggttt gcgccggttt gggcgctgtt gcgactgctt tctttgtatt
     5101 cggcagaaca ggaagcgctt tggcagctcg cgatctgaga gatcaccgtc cgtccggcag
     5161 agccggcgca ttgtcttcgc aacgggaatg accgcaagtt tgcaattgaa gaagcaccgt
     5221 tgtcagggta aattgcagga taattgtttt ccatacattt acccagctgc cgactcaccg
     5281 cagtttatgt ccatgaggat tcaacccggt gactccatc
//
LOCUS       DASUTG010000430         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1400756, whole genome shotgun sequence.
ACCESSION   DASUTG010000430 DASUTG010000000
VERSION     DASUTG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400756"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1076)
                     /locus_tag="VF089_09860"
     CDS             complement(120..1076)
                     /locus_tag="VF089_09860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174309.1"
                     /translation="MEGKPWPIPSGSGVGIGGPYSRPHPRRDGMKDPNSRLADMDNEG
                     IDLAVLFGGGIAGTIPALENAGFATALARARNDWLAEYCAANPSRLKGVAVLPQQDIA
                     ASVAELKRTVSELGFIGVSLLPNLRGRHLGDPCFFPIYEEAQRLNVPICVHMFLGRYG
                     SEATGTTRFDKFFYVHLFGHPFEQMIALSVLAGEGVLDRFPKLRFVFLESGCGWVPYW
                     LDRLNEHYEVLGVQLPALKTEPGKLLQRGQLYFSCEPDETELPHVVDTIGDDWIVFAS
                     DYSHFDSRFPGASEPIVENQKLNDLSKRKILNDNARRLYPLG"
     gene            complement(1076..1213)
                     /locus_tag="VF089_09865"
     CDS             complement(1076..1213)
                     /locus_tag="VF089_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174310.1"
                     /translation="MSKFRTVDADGHVEEAHVNWVDRIAEPYETWRPNRVRRPIAIGA
                     L"
     gene            complement(1418..2401)
                     /locus_tag="VF089_09870"
     CDS             complement(1418..2401)
                     /locus_tag="VF089_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174311.1"
                     /translation="MMLPALLIILVVAVIPSELRGQERYSVSYGGFAGYHAPLWAAKD
                     LGLFAKHGLNADPVMIAGSARGMQALLSGSTHFALADATGPVSALYQGADVVLVASAM
                     NKFPFSMYAQKEITRHADLKGKRIGIASFGGATEWGLNLGLREWNVPRDSVTVTAHGP
                     GANRLLALSTKGVDAALLAPPESAQAEREGMRFLGHLSELKANFPMAVVVVRRAFLEK
                     NRDPVKRFIQAYSEAIHEFKTDKDKMIAVYNQRLKQRNPQVIEQTYSYFAPIFFLPPR
                     VPLDGARYTVELIAQRSPPDKLPPVVEQFVDNSIVDELEREGFFKKLGARR"
BASE COUNT          535 a          703 c          726 g          522 t
ORIGIN      
        1 agcgagggaa tgccgacctt cacgttttca gcgtttgctt gggcgcagag ttgcggcacg
       61 gcaaggatca gcgacgctaa ataggcgatg acaatcgatc ggcgagtcat agccgctcct
      121 taaccaagcg gataaagccg gcgcgcgttg tcgttgagaa tcttgcgctt ggagaggtcg
      181 ttgagcttct ggttttctac aatcggctcg gaggcgccgg gaaaccggct gtcgaaatgg
      241 ctgtaatccg aggcgaaaac aatccagtcg tcaccgatcg tgtctaccac gtggggcagc
      301 tcggtctcat ccggctcgca tgaaaaatag agttgcccgc gctgtaacag cttgccgggt
      361 tcggtcttga gcgccggcag ctggacgcca agcacctcgt agtgttcatt gagacgatcc
      421 aaccagtaag gcacccagcc gcagccggat tccagaaaaa caaatcgaag cttcggaaag
      481 cggtccaata cgccttcgcc ggcgagcacc gataatgcga tcatctgttc gaacggatgg
      541 ccgaaaagat ggacataaaa aaacttgtcg aatcgtgtcg tgccggtcgc ctcggagccg
      601 taacggccga gaaacatgtg tacgcatatc ggcacgttca aacgttgggc ctcttcgtag
      661 atcggaaaaa aacaaggatc gcccaagtgc cggccgcgca ggttgggcag cagagacacg
      721 ccaataaatc ccaactcgct gaccgtgcgc ttcagttcgg caacggatgc ggcgatatcc
      781 tgttggggaa ggacggcaac gccttttaat cgggacggat tcgccgcgca atactcggcc
      841 agccaatcat tgcgagctcg cgccaaggca gttgcgaagc ctgcgttttc aagagccgga
      901 atggttcccg caatgccgcc accgaaaagc acggcgagat caatcccctc attgtccatg
      961 tcggcaagcc gggagtttgg atctttcatt ccgtcccgtc gcggatgtgg acggctgtaa
     1021 ggtccaccga tgccaacgcc tgatccgctc ggtatcggcc acggtttccc ttccatcaca
     1081 aggcgccaat ggctatcggt cgccggacgc ggttggggcg ccatgtttcg tacggctcgg
     1141 ctattcgatc tacccaattg acgtgagctt cttcgacatg gccgtcagca tcgacagtgc
     1201 ggaacttgct catagagcct cctcgaggac agcttgggtg aatttgcctt cgctatttac
     1261 cgccatcaaa tacgtgaaat caatgcgagg tccttgccgc actagcgcgc tttgtgcaac
     1321 cgagtgcagc aagaatccgg tggcaggcac gattgacgaa ctctcacgtg gagcaaaaaa
     1381 tacgttttac tgcggcctag caaatcgaat ggatttttta gcgccgcgcg ccaagtttct
     1441 tgaagaaacc ttcccgctcc agctcatcaa cgatgctgtt atcgacgaac tgctcgacca
     1501 ccgggggcaa cttgtccggc ggcgagcgtt gcgcaatcaa ctccaccgta tagcgggcgc
     1561 cgtcgagcgg cacgcgcggc ggtaaaaaga aaatcggcgc gaagtaactg taggtctgtt
     1621 cgatgacttg gggatttctc tgtttgagcc gctggttgta cacggcgatc atcttgtctt
     1681 tgtcggtttt gaactcgtga atcgcctccg aataggcttg gatgaaacgt ttgaccgggt
     1741 cgcggttttt ttccagaaag gcgcggcgca cgaccaccac cgccatcggg aagttcgcct
     1801 tgagctcgct caaatggccg agaaaccgca ttccttcgcg ctctgcctgg gccgattccg
     1861 gaggcgctaa cagtgccgca tcgacgcctt tagtcgacag cgccagaagc cggttggcgc
     1921 cgggaccgtg agcggtgacc gtgactgaat cgcgggggac gttccattcc cttagaccaa
     1981 ggttaagccc ccattccgtg gcgccgccga aactggcgat gccgattcgt ttgcccttga
     2041 ggtcggcgtg ccgggtgatc tctttctggg cgtacatgct gaaggggaat ttgttcatcg
     2101 cgctggctac gagaacgaca tcggcgccct gatagagcgc gcttaccgga ccagtggcat
     2161 cggcaagggc aaagtgggtg ctgcccgaaa gcagcgcctg catgccccgc gccgaaccgg
     2221 cgatcatgac tggatcggcg ttgaggccgt gcttggcaaa gagacccagg tccttggcag
     2281 cccataaagg cgcgtgatag ccggcgaagc cgccatagct gaccgaatag cgctcctgcc
     2341 cgcggagctc ggatggaatc accgcgacga caaggattat taataaagcc gggagcatca
     2401 ttgttttctc cttcctgcgg cttaatcgcc ggcattagat aatcgcgtga tgttgcttgt
     2461 caagcgccgg atgttttcca tcttcc
//
LOCUS       DASUTG010000431         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1846152, whole genome shotgun sequence.
ACCESSION   DASUTG010000431 DASUTG010000000
VERSION     DASUTG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846152"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1583)
                     /locus_tag="VF089_09875"
     CDS             complement(54..1583)
                     /locus_tag="VF089_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6174312.1"
                     /translation="MDSNGDGSGDLRGIINRLDYCRWLGVAAVWLSPIYPSPMIDFGY
                     DIADFTAIHPLFGTLTDFDVLISEARRLSLKVLLDYVPNHSSDQHPWFLQSRSSRNNP
                     YRDWYIWRDSAHGGAPPNNWLGAFGGSAWEWDENTGQYYLHSYLKEQPDLNWRNPDVQ
                     QAMLEVLRFWLDRGLSGFRVDSMAHLMKDTQFRDNPVNPDWDPATGPYFRYLPVHSRD
                     HPDSAAVIRRIRQVIDEYEDCVLIGEAYLSLERTVAYYGAGVHLPFNFALITSPWSAT
                     EVHRIIKTYESLLGFDQWPNWVLGNHDRHRIATRIGRAQARVAAMLLLTLRGTPTLYY
                     GDEIAMQDVPIPAELVRDPWESNVPALGLGRDPERSPMQWDSTDHAGFSTATPWLPVA
                     NDFRRFNVAIEAQEADSVLTLYRRLIALRAEKSALHSGAYGELSCDDHVLSYGRQSGA
                     GGFLIVLNFTGESRTFAYPGLRGRILLTTALDRNSETVAQNLLLRPDEGVIIEKEHGG
                     V"
     gene            complement(1695..>2672)
                     /locus_tag="VF089_09880"
     CDS             complement(1695..>2672)
                     /locus_tag="VF089_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6174313.1"
                     /translation="EELQRIKSLAIPPAWREVWICPLRNGHLQATGRDAKGRKQHRYH
                     PRWRQVRDENKYGRILAFAKALPLIRQRVDGDLRLPGLPRDKILATVVRLLETSHIRV
                     GDEEYARQNKSYGLATLRNRHVNVSGSTIRFEFRGKSGVEHALDINDRRLAKIIKRCQ
                     DLPGYELFQYIDDDGERRTIDSTDVNDYIRQIAGEDFSTKDFRTWAGTVLAARALREF
                     ENAASASESKSNIVHAIESVAKKLGNTKAVCRKCYIHPAVIQSYTDGSLLRALRRRLK
                     EEANDSLGLDPEEAAVLKLLKAGISQESKAAPKLHAIEREASARLAHAK"
BASE COUNT          598 a          717 c          749 g          608 t
ORIGIN      
        1 gagcctgcga gatgctaagc aacggacctg gtgagtttta gacggcgcgc tgcttaaact
       61 cctccatgtt ctttttcgat tatgacaccc tcgtcaggtc gcagtagaag gttttgggca
      121 acagtttcac tattgcgatc gagagctgtg gttaagagta ttcggccacg aagaccgggg
      181 tacgcaaatg ttctagactc tccggtgaaa tttagtacga tcaaaaaacc tcccgccccg
      241 gattggcggc cataactcag aacatgatcg tcgcaggaaa gttcgccata ggcgccgcta
      301 tgcagtgccg atttttcagc ccggagcgcg atcaaacggc gatagagtgt gagaactgag
      361 tcggcttcct gagcctcgat agcaacattg aagcgtctaa aatcattggc caccggcaac
      421 catggagtgg ctgtgctgaa gcccgcgtga tcggtcgagt cccattgcat cggcgagcgc
      481 tccggatctc gacctaagcc gagggcaggg acgtttgact cccagggatc tctgaccagt
      541 tcagcaggga tcggaacatc ctgcatcgca atttcatcac cgtagtagag cgtcggcgtg
      601 ccgcgcaaag tgagcagaag cattgccgcg acgcgcgcct gggccctgcc gatgcgagtg
      661 gcaatgcggt ggcggtcgtg atttcccagc acccaattgg gccactggtc aaatccgagc
      721 agtgactcgt aagttttgat tatgcgatgc acctcggtgg cgctccaggg actggtgatc
      781 aaggcgaagt taaaagggag atgaactccg gccccgtaat aggcaacggt gcgctccaaa
      841 gaaaggtagg cttcgccgat aaggacgcaa tcctcgtatt catcgataac ctgccgtatg
      901 cggcggatga cggctgcgct gtcaggatga tctcgggagt ggaccggcag ataacgaaaa
      961 tacggaccgg tggccggatc ccagtcggga ttgaccggat tgtctcgaaa ctgagtgtct
     1021 ttcatcaagt gagccatcga gtccactcgg aaaccgctga ggccccggtc aagccagaat
     1081 cgcagcacct ccagcattgc ctgctgaacg tcaggattgc gccagtttaa atcgggctgc
     1141 tctttcaggt aggagtgcaa gtaatattgt ccggtgttct catcccactc ccaagcgctt
     1201 ccgccgaatg cgcccagcca gttgttgggc ggcgcgccgc catgcgctga atcgcgccag
     1261 atgtaccagt ctcggtaggg attgttccgg gacgatctgg actgaagaaa ccagggatgt
     1321 tggtccgagc tgtgattggg cacataatcc aggagaactt ttaggcttag acggcgcgcc
     1381 tcgctgatca gcacgtcgaa atcagtgagg gtgccaaaca gcggatggat agctgtgaaa
     1441 tcggctatgt cataaccaaa atcaatcatc ggcgaaggat agatcggcga tagccaaacc
     1501 gcagcgaccc cgagccaccg gcagtaatca agccgattga taattccgcg caggtccccg
     1561 ctgccgtcac cattgctgtc cataaatgac agcggataga tttgataaat cagacctttt
     1621 ttccaccaac gagtttcgct aggccctgct gaagcaaccg gggcggaaga aatcatctca
     1681 tcagttatgt cggattactt tgcgtgcgcc agtcgagcgc tagcctctcg ttcgattgcg
     1741 tgcagctttg gcgctgcttt actttcctgg ctgatgccgg cttttagaag cttcagcaca
     1801 gcggcctctt ccgggtcgag accaagtgag tcgttggctt cttccttgag ccgccggcgc
     1861 agcgcgcgca acagagatcc gtccgtatag gactgaatca ccgccggatg aatatagcat
     1921 ttgcggcaca cagctttggt attgcccaat ttcttggcaa cggattcgat cgcgtgaact
     1981 atattgcttt tgctttctga agcagacgca gcattctcga attctcgcaa tgctcgcgcg
     2041 gccaagaccg tgcccgccca cgtgcggaaa tctttagtgc tgaaatcttc accggcaatc
     2101 tggcggatgt aatcgttaac atcggtggag tcgatggtgc gacgctcgcc atcatcgtcg
     2161 atgtattgaa acagctcata gccgggaaga tcctggcagc gcttgatgat ctttgctaag
     2221 cgtcgatcat tgatatcgag cgcatgttcg acgccgctct tgccgcgaaa ctcgaaacga
     2281 atcgtcgaac ccgagacatt gacatgacga tttctcaggg ttgccagccc ataagattta
     2341 ttttgccggg catactcctc atcgccgacc cgaatatggc tggtttcgag cagtcttacc
     2401 accgttgcca ggattttatc gcgtggtaag ccgggcagcc gaagatcgcc gtcgactctt
     2461 tggcgtatga gcggaagagc tttggcaaaa gccaaaatac gaccgtattt gttttcatcc
     2521 ctgacctggc gccatcgcgg gtgataacga tgttgttttc tgcctttggc atcgcgcccg
     2581 gtcgcttgca ggtggccgtt gcggagcgga cagatccaaa cctcacgcca agccggtggt
     2641 atcgccaagg atttgatgcg ttgcaattct tc
//
LOCUS       DASUTG010000432         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1846367, whole genome shotgun sequence.
ACCESSION   DASUTG010000432 DASUTG010000000
VERSION     DASUTG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846367"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF089_09885"
     CDS             complement(<1..401)
                     /locus_tag="VF089_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174314.1"
                     /translation="MKISFFGSSLLSAYWNGAATYYRGILSALFERGHQIVFYEPDAW
                     DRQRHRDLTETPYAQSVVYPVGDDSAVLEIVKKAARESDLLVKASGVGVFDALLEEAV
                     LDLRRPDSIVAFWDVDAAATLERIAQNTADP"
     gene            complement(398..1537)
                     /locus_tag="VF089_09890"
     CDS             complement(398..1537)
                     /locus_tag="VF089_09890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6174315.1"
                     /translation="MNIALFYHSLVSDWNHGNAHFLRGIAGELQRRGHTLRIFEPAGG
                     WSLTNLLREYGQAPLEAFAKIYPGLHSSFYDPATFDLDRALDRMDLVIVHEWNHPALV
                     RRIGAHHKTSCDYRLLFHDTHHRSVTEPEKMAAYDLADYDGVLVFGKAIAEIYRTRNW
                     ARRVWVWHEAADTTIFIPLPGLQKEGDLVWIGNWGDEERSAELHEFLLEPVKALGLKA
                     RVYGVRFPADALGALSAAGIDYGGWLPNFQAPATFAGFRLTVHVPRRPYVRALPGVPT
                     IRPFEALACGIPLVCAPWEDSEHLFSPGKDYLVARDGAEMKQHLQSVLADPQLAGALS
                     FHGRKTILQRHTCAHRVDELLEIYHSIDSPLSSAEAMERAPEKHL"
     gene            complement(1537..2667)
                     /locus_tag="VF089_09895"
     CDS             complement(1537..2667)
                     /locus_tag="VF089_09895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6174316.1"
                     /translation="MLLPTRPILMTADPIGGVWQYSFELCRELAKRDVSVVLAVMGRR
                     LERGERVLMGGLHNVELCESTYKLEWMSEPWSDVEAAGQWLLNLESRFQPSLIHLNQY
                     SHGALAWKAPALVVGHSCVYSWFEAVKGAPPGPEWQSYRAAVRLGLQGADLVTAPSRS
                     MLASLEKHYGRFAAAEPIYNGRSGARFAPGRKEDFIFTAGRLWDEAKNIASLNSIVGK
                     IPWSIYAAGESRAPDGRPPLVDGPVLLGRLDSEMVAHWFSRAAIFILPARYEPFGLSA
                     LEAALSGCALVLGDTESLREIWHDAALFVRPEDPEEICATLLELIAKPSLRDDFSRRA
                     RKRAGSFTAERMARAYMGVYAKLLRSQRGLLSEEVIQGEHPL"
     gene            complement(2686..>3655)
                     /locus_tag="VF089_09900"
     CDS             complement(2686..>3655)
                     /locus_tag="VF089_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6174317.1"
                     /translation="VGWIGRHRLQSIIESGAVEIAAVADLDSAAASAVAAQSPGAVAA
                     PSLDALLELQLDGLVIATPTALHAEQAIAALEKETAVFCQKPLGRSADETARVIRAAR
                     LANRLLAVDLSYRFVRGVQKIKQMISEGEIGTIYVADLVFHNAYGPDKAWYYDREFSG
                     GGCLIDLGIHLIDLALWMLDFPGVENVSSRLFSQGRPVEHVPEALEDYALARLDLASG
                     ATVNLACSWRLPAGRDAVIRASFFGTAGGLSIQNVGGSFYDFRAERFQGTSRVILDQP
                     PDAWGGRAAVAWAEQLSADATFDAQVERAYSVAQVIDEIYRQNVSG"
BASE COUNT          798 a         1146 c         1027 g          684 t
ORIGIN      
        1 aatggatcgg cggtattctg agcgattcgc tccagtgtcg ccgcggcatc gacatcccag
       61 aaggcaacga tgctgtcagg acggcgcaag tccagaacag cctcttcgag gagcgcatcg
      121 aagacaccca cgccgctggc cttaaccaac agatccgact cgcgcgcggc tttcttgacg
      181 atctcgagta cggccgagtc atcgccgacc ggatacacca ccgattgcgc gtagggggtt
      241 tcggtgagat cccgatgacg ctgccgatcc caagcatcag gctcataaaa aacgatctga
      301 tgtccccgct cgaaaagcgc gctcaaaatg ccgcggtaat aggtcgccgc gccgttccag
      361 tatgccgata gcagactcga gccaaagaaa gaaattttca tagatgcttc tcgggtgctc
      421 gttccatggc ctcagccgac gacagcggcg agtcaatgct gtgatagatc tccaagagtt
      481 cgtcgaccct gtgggcgcag gtatgacgct gcagaatggt cttacgccca tggaaggaca
      541 atgcgccggc gagctgagga tcagcaagca cgctttgcaa gtgctgcttc atctctgcgc
      601 cgtcgcgtgc gaccaggtaa tccttgccgg ggctgaataa atgttcgctg tcttcccatg
      661 gcgcgcaaac tagaggaatg ccgcacgcca gcgcttcaaa cggccggatt gtcggaacgc
      721 cgggcaaagc ccgcacgtag ggccggcgcg ggacgtgaac cgtcaagcgg aagccagcga
      781 atgtggccgg cgcttggaag ttaggcaacc agccgccgta gtcgataccg gcggccgaaa
      841 gcgctcccaa tgcatctgcc ggaaaacgca cgccgtaaac acgcgccttc agacccagcg
      901 cttttaccgg ctcaagcaga aattcatgca gttccgctga tcgttcttca tcgccccagt
      961 taccgatcca gaccagatct ccctctttct gcagaccggg gagcggaatg aaaatggtcg
     1021 tatccgccgc ttcatgccac acccaaacgc gcctcgccca attgcgcgtg cgataaatct
     1081 ccgcaatcgc tttgccgaaa acgagaacgc catcataatc ggccaagtcg taggccgcca
     1141 ttttctcggg ttcggtcacc gaccggtgat gcgtgtcatg aaacagcagc cggtagtcac
     1201 agctggtttt atggtgcgcg ccgattcgcc gcaccaaggc tgggtgattc cattcgtgaa
     1261 caatgacgag atccatgcga tcgagcgcgc ggtccaggtc gaaagttgcc ggatcgtaga
     1321 agctgctgtg caagcctgga taaatctttg cgaatgcctc gagcggcgct tggccgtact
     1381 ctcgcaacaa gttggtcagg ctccatccgc ccgccggttc aaagatccgc agtgtgtggc
     1441 cgcgccgctg aagctctccg gcgatgccgc gcaaaaagtg cgcgttgcca tgattccagt
     1501 ccgaaaccag cgaatgatag aaaagagcaa tattcattat aatgggtgtt cgccttgaat
     1561 aacttcttcc gagagcaggc ctcgttggct tcgcagtagt ttggcataga cccccatata
     1621 cgcccgcgcc atgcgttccg ctgtgaaaga acccgcccgt tttctcgcgc gccgggaaaa
     1681 atcatcgcgc aaagacggct tggcaatcaa ctcgagaagc gtcgcgcaaa tttcctcggg
     1741 atcttccggc cggacaaaaa gcgctgcgtc atgccatatt tcgcgcaggc tttcagtgtc
     1801 tccaagaacc agggcacagc cggagagcgc cgcctcgagc gcggataagc caaatggctc
     1861 gtagcgtgcc ggcaaaataa aaatcgccgc gcggctaaac cagtgcgcga ccatttccga
     1921 atcaaggcgg cccaataaaa ccggcccgtc caccagcggc ggtcttccgt cgggcgcgcg
     1981 gctctctcca gcggcgtaga tcgaccaggg aatctttccg acgatggagt ttaggctcgc
     2041 aatgttcttg gcctcgtccc acaggcgacc ggcggtaaag atgaagtcct ccttcctccc
     2101 cggagcgaag cgcgcgccgc tgcggccgtt gtagataggc tctgctgcgg caaaacggcc
     2161 ataatgcttt tccaaggagg ccagcatcga gcggctcggc gctgtcacca aatcggcgcc
     2221 ttgcagaccc agtcgcacgg cggcccggta ggattgccat tccggaccgg gcggcgcgcc
     2281 tttaacggct tcaaaccatg agtaaacaca cgagtggccc accaccagag ccggggcctt
     2341 ccaggcgaga gcgccgtggc tgtattgatt tagatgaatc aggcttggct gaaagcgtga
     2401 ttcgagattg agcaaccatt ggcccgcggc ttcgacatcg ctccacggct cgctcatcca
     2461 ctcgagcttg tacgtgcttt cgcacagctc gacgttgtgc aaaccgccca tcaagactcg
     2521 ctcgccccgc tcgagccgcc ggcccatgac tgcgagcacc acgctgacgt ctcttttggc
     2581 caattcccgg cacagctcga aggagtattg ccagactccg ccaatgggat cggcggtcat
     2641 gagtatcggc cgcgtcggta acagcactcg cagtatccct ctccttcaac cggaaacatt
     2701 ctgccggtag atttcatcga tcacttgagc cactgagtaa gcccgctcaa cttgcgcatc
     2761 aaaagtggca tcggcgctca gctgctcggc ccatgcaact gcggcccgcc cgccccatgc
     2821 atcgggcggc tggtcgagga tcactcgact cgtgccttga aagcgttcgg cgcgaaaatc
     2881 gtagaaagaa ccgccgacgt tctgtatgga caaaccgcct gcggtgccga agaaagatgc
     2941 acgaatcacc gcgtcccgtc ccgccggcag ccgccaggag caggccagat tgacggtggc
     3001 gccgctcgcc agatccagac gcgccagcgc gtaatcttcg agagcctctg gcacgtgctc
     3061 gaccggtctg ccctggctga acaggcggct cgagacattc tcgactcccg gaaaatcaag
     3121 catccataac gccagatcaa tgagatgaat gcccagatcg atcaggcaac cgccaccgga
     3181 aaactcccga tcgtaatacc aggccttgtc tggcccatag gcgttatgaa agaccaaatc
     3241 ggcgacatag attgtgccga tctccccctc gctgatcatt tgtttgatct tttgaacccc
     3301 gcggacaaag cgataagaca ggtcgacagc taagagccgg ttggccagcc gcgcagcacg
     3361 aatgacccgc gccgtctcat cggcgcttcg tcccagcggc ttctggcaga aaacggccgt
     3421 ttccttctcc aatgcggcaa tagcttgttc ggcgtgtaag gccgtgggcg tggcgatcac
     3481 cagaccatcg agctgcaatt cgagcaaagc atcgagcgac ggcgccgcca ctgcacctgg
     3541 agattgcgcc gcaaccgcgc tggcggcagc gctgtcgaga tcggccactg cggcgatctc
     3601 caccgcgccg ctttcgataa tcgattgcag ccgatgacga ccgatccagc cgacg
//
LOCUS       DASUTG010000433         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1697102, whole genome shotgun sequence.
ACCESSION   DASUTG010000433 DASUTG010000000
VERSION     DASUTG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697102"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VF089_09905"
     CDS             <1..370
                     /locus_tag="VF089_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174318.1"
                     /translation="VVEEAKKFACDLRRFYLTGFSFGGNGVFDLSFAQTAMWAALWPV
                     DPTRAPTKPLTIPVWLSLGEISRLHARSFIRTLGLDAAETGPAGDWLWFDAGEDHVGT
                     ATQAYDDERVYRWLLSKHLQ"
     gene            complement(458..817)
                     /locus_tag="VF089_09910"
     CDS             complement(458..817)
                     /locus_tag="VF089_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174319.1"
                     /translation="MAVILFVEDEAVSRRSIAHFLRLSGYEVHEAENGEAALRLLATM
                     QFDVVISDLNLPGKLNGIDILAALKKIPGKIRAILMTGRGSEEIKSKANSLGAAYLEK
                     PVQLQELETALQRRAIR"
     gene            1338..1607
                     /locus_tag="VF089_09915"
     CDS             1338..1607
                     /locus_tag="VF089_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174320.1"
                     /translation="MVVVIGFARADYERLLQNVPAEAPAFAVLRRCPELSRWGREEPL
                     FKCVIIECNPEEAAVLLDSAKQYCPRAVADIEYGLKLSRAFDLLS"
     gene            complement(1600..1986)
                     /locus_tag="VF089_09920"
     CDS             complement(1600..1986)
                     /locus_tag="VF089_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174321.1"
                     /translation="MKILIIEDNSDMRDLVCLILERLFYVPLRASHGREGLEKAITEK
                     PQLILMDMRMPVMDGWEAARALRTHPDTKNIPILATTALFRPHELRTCLEAGCSDYIV
                     KPFSCVDLQTKIGEMLRPSTEQLRFN"
     gene            complement(2355..3668)
                     /locus_tag="VF089_09925"
     CDS             complement(2355..3668)
                     /locus_tag="VF089_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEX6174322.1"
                     /translation="MQTLSRGNWTGAAGFILASMGSAVGLGNVWRYPYITGQYGGAAF
                     ILLYIGCVLSVGIPLMIAEFLIGRKTQRNPVGAFRKLRPQSPWVLTGWLGIVSAFTIL
                     SFYAVVGGWVLHYVHLSLVNSFTGKSAEQVTDIFTSMNVSPGLQILWLAIFMLVTIVI
                     VSKGVSSGLEAGNKIMMPLLFALLIVLAIYATQTSGAAPGLEFLLRPRWQRIGPTAVL
                     EALGQALFSLSLGMGTMITYGSYLSRQSNLVRPALLIATGDTLVAVLAGFVVFPLAFS
                     FGLEPGAGPGLIFRTLPIAFAQLSGGYWVSLAFFILLSFAAITSSISLLEVASAYFID
                     EQHWSRNRAAWTLGIVIFLLGIPSAMGEHFLGVMDALATNYLLPIGALLIAVFTGWVL
                     RDNERNDQFSGSNITAARLAVWSFLIRFVTPVAVILIFLHQLRVF"
     gene            complement(3966..4397)
                     /locus_tag="VF089_09930"
     CDS             complement(3966..4397)
                     /locus_tag="VF089_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174323.1"
                     /translation="MSGAIKKTQLFKRRRSEQRIAAEDWSYPTGMVWCPMKMSGMALM
                     KCAEMQKQLGCGSIKQFKIVTITKPAHVLLYWPWLRRGRECPERADEKEVRELRLALS
                     PLKLVEKSRKNPRGYCCPACGGRKAFGARQCRSCWRLSLGQ"
     gene            complement(4650..>5255)
                     /locus_tag="VF089_09935"
     CDS             complement(4650..>5255)
                     /locus_tag="VF089_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174324.1"
                     /translation="WTLKDAMRTRTLWLLVAAASFYSLSTGGIAFHMVAYFTDVTIKP
                     AVAAGALSVMALTGAFGSGLWGALAERFHPRTLGVTTMLLAAAAVVLLTQVRSAPAAF
                     VFSILFGLSARGGFVLMHVLLARYFGRRSFGAITSVLEPFHKGGLGVGALLAGMAFDV
                     SGSYHIVFLVFIANYLIAAMLTCLAREPSYAPRDRHTAASS"
BASE COUNT         1283 a         1418 c         1406 g         1148 t
ORIGIN      
        1 agttgtggag gaggctaaaa aatttgcttg tgatctgcgc cggttttatc taaccggctt
       61 cagctttgga ggaaacgggg ttttcgatct atcattcgca caaaccgcaa tgtgggccgc
      121 cttatggccg gtggacccta cccgggcgcc aactaagccg ctcaccatac cagtctggct
      181 ctcgctcgga gagatttccc gcttgcacgc gcgcagcttc attcgcaccc tgggcctcga
      241 tgcagcggag accggcccag caggcgattg gctgtggttc gatgcaggtg aggatcatgt
      301 cggcaccgcc actcaggcgt atgacgatga acgggtttac cgttggctcc tgagcaaaca
      361 cttacagtag agacttatgt cgatgtgcat gagtttcaag ctcgtggatc tcgtgtcggg
      421 aattgtccaa tatcgcttat gatcctcgat gcgctggcta acgtatcgct ctgcgctgga
      481 gggcggtttc gagttcttgc agctgaactg gtttttccaa gtaagcagcg cctaaggaat
      541 tagctttact tttaatctct tcggagccgc ggcccgtcat caggatggct cttattttac
      601 cgggaatttt cttcagagca gccaatatgt cgataccgtt tagctttcca ggcagattca
      661 ggtcacttat cactacatcg aactgcatcg ttgccaacag cctgagggct gcctcgccgt
      721 tttctgcctc gtgaacttcg tacccggata gcctaaggaa atgagcaata ctgcggcgtg
      781 atacagcctc gtcttccacg aagagaatga cagccatgga cgcacccccg ctagagaggt
      841 agcaatttcc acgcccacgg acacgtggtc acgttctctg ggttttcaca gttcgcagca
      901 aagtcgagcc tgccagcggc cgcatgacgg tagcagagct gaaattgaac aaagtcaact
      961 tagtttcagc agcggtacag tttggctgcg cgtcacagcc ctgactttat acgccgaccg
     1021 agctgggccg ccagtctgcg acgactagcc ttactcgcag gctggtgcag catctatgat
     1081 cgatttagct catcttaatc gtgaaccgct caacaatatc cgacgatcac atgaacagca
     1141 ttttgtgtgc ggtattcatt tggtaacgtc aaagttgacg tcctttcaag catcatttcg
     1201 ttgtatttcc taaaaattac ccggcctgtt tgttgctggc cctgtgcctt ccataggagg
     1261 acaccgctga gaacgtttac tgaccagatt gaggcttacg cagttctgca gacgtgtgtc
     1321 aaagattcta aagccatatg gtagttgtga ttggattcgc gcgcgctgac tacgagcggt
     1381 tgctgcagaa cgttcctgcc gaagctcctg cctttgctgt tctccgtcgc tgcccggagc
     1441 tctcccgttg gggccgtgaa gaaccgcttt tcaaatgcgt gattatcgaa tgcaatccgg
     1501 aggaggcagc agtgctctta gactccgcca agcagtattg tccgagggcg gtagccgaca
     1561 ttgaatacgg actcaagttg tcccgcgcct tcgatctgct cagttgaatc gcagttgttc
     1621 agtactcggt cttaacattt ctccgatttt ggtttggaga tcgacacagc taaacggttt
     1681 gacgatgtag tcgctgcagc ctgcttccaa gcaggtcctt agttcatgag ggcgaaacag
     1741 cgcagttgtt gccaagatcg gaatattctt cgtatcagga tgagtccgca gggctctggc
     1801 cgcttcccag ccgtccatta ccggcatcct catgtccatc aggatcaact gcggcttctc
     1861 tgtgatagcc ttttccaacc cttctctacc atgagacgcg cgaagcggaa catagaagag
     1921 ccgttccaga ataagacata ctagatcacg catatccgaa ttatcctcga tgatcaaaat
     1981 cttcattttc tcacctttgt taggattgat cggcgtttat cttcaacacg ccaaaaattg
     2041 tggcgcggta aatatcacaa atattttttc tttcaaggga agcctaagag caccatcaag
     2101 acgcctctat ggcgtaaatc tctcgacgcc gcgcgaagcc gttgcagatt taccgaccat
     2161 cgtgcaggtg acttctagat ctgacaccga acgggcgcac cacttacacg ggagggccat
     2221 gcaagtcaca gccgagtgag tttactcctt gcacaggaga cactgcgggc cgcgccggcc
     2281 gtgcttcgct aactgctccg tcagagagtt agaactcgat gcagccctcg ctgacgcacg
     2341 gctatgtgga gaggctaaaa tacgcggagt tggtgaagaa aaatcaggat aaccgcaacc
     2401 ggagttacga accggatcaa gaatgaccag accgccaggc gggcagcggt gatgttgctg
     2461 ccgctgaact gatcgttgcg ctcattgtct ctcaacaccc agccggtgaa aactgcgatc
     2521 agcaaggcgc cgataggcaa gagatagttt gtcgcgagcg cgtccatgac acctaagaaa
     2581 tgttcgccca ttgcggaggg aatccccagc aagaagataa cgattcccaa ggtccatgcg
     2641 gctctgtttc gcgaccaatg ttgttcatcg atgaaatacg ctgatgcgac ctccaatagc
     2701 gaaatcgagg atgtaatggc ggcgaagcta agaagaatga aaaacgccag agacacccaa
     2761 taacctccgg acagttgagc aaaggcgatg ggcaaggtgc gaaagatcaa ccccgggcct
     2821 gcaccgggtt cgagaccgaa actaaacgcc agcggaaata cgacaaaacc ggcaaggacc
     2881 gcaacgagcg tatcgccggt cgctatcaga agcgccggcc tgaccagatt gctttgacgg
     2941 gaaaggtaac tcccataagt aatcatcgtc cccattccta aactcaaaga gaaaagggcc
     3001 tgccccaggg cttccagtac ggcggtggga cctatccttt gccaccgcgg gcgcagcagg
     3061 aactcaagtc ccggtgccgc gccgctggtt tgcgtcgcat aaatcgccag aacgatgagc
     3121 agcgcaaaca gtaacggcat catgatctta ttaccggctt cgagaccgct cgaaactcct
     3181 ttagatacga tgacaatcgt gacgagcata aaaatggcga gccagaggat ttgcaggcct
     3241 ggactgacat tcatactggt gaaaatatcc gtgacctgct cggcagattt gccggtgaag
     3301 gagttcacca gcgaaagatg aacataatgc aaaacccagc cgccgactac ggcataaaag
     3361 gagaggatcg tgaaagcgct aacgatgccc aaccagccag tgagcaccca tggcgattgt
     3421 ggccggagct ttctaaacgc gccaacagga ttgcgttgag tttttctgcc gatgaggaat
     3481 tccgcgatca ttaacgggat accgacagac aggacgcatc cgatataaag aagaataaaa
     3541 gcggcgccgc cgtactgccc cgtgatatag ggatagcgcc atacgttgcc caggccgacg
     3601 gcggatccca tcgacgccag gatgaaacct gcggccccgg tccaatttcc gcgcgataaa
     3661 gtttgcacca gcctctccta gaaggatgtc atggaagtct ttagccggcg ctatggcaat
     3721 gctccgcgcc agcggttgtt atattatatc gagatttata gccggggctc aggagcggca
     3781 caataacttg cttcggcgaa gcaagagcgc ccaggaaaca ttctcaatcg aggcgaggtg
     3841 cttgacaaga aggtgcttga caagaagata gtggactatt gatccgcgcg gcgtaactga
     3901 aggcgcaaaa ccgagagcga ccttcgaaag gactacgtaa gcggggaaac tcaaccagtc
     3961 tctgctcatt gtccgaggga aaggcgccag cagctgcgac attggcgcgc gccgaaagct
     4021 ttgcggccgc cgcaagcggg gcaacagtag ccgcggggat ttttcctgct cttctccact
     4081 aacttgagcg ggctcagagc caagcgcaat tcacgcactt ctttttcatc ggcgcgttcc
     4141 gggcactcgc gaccccgccg cagccagggc caataaagca atacgtgggc gggtttggtg
     4201 atggttacga ttttgaactg cttgatgctg ccgcagccga gttgcttttg catctcggcg
     4261 catttcatca gcgccatgcc gctcattttc atagggcacc agaccattcc ggtgggataa
     4321 ctccagtcct cggcggcaat tcgctgctca gagcgccgcc ttttaaacag ttgtgtcttt
     4381 tttattgcgc cagacattct ttcacggaac tttctgcgtc tcagtatgcg taaaatcctt
     4441 gagatcgcaa gcgagaagca gggcaaaaaa caacccgaac atgttttctt tgggtaataa
     4501 acttacagtc gaaaaatcac aagagaatag gaacattttt tggtgcccgt gaagcggcgc
     4561 cgtattgagt cacgctcctg gttcagtcgt gggctgtcag ttgcggaatg tcacagcgtg
     4621 atttctagcc gtgaatctgc cgcaaacgct caactgctgg ctgccgtatg ccggtcacgc
     4681 ggagcataag acggctcgcg agccaggcag gtgagcatgg cggcgatcag ataatttgcg
     4741 ataaaaacta gaaaaacaat gtggtagcta ccgctcacat cgaaagccat tcctgccagc
     4801 aaagctccga cgccgagccc gcctttatga aagggctcaa ggacgctggt gatggcgccg
     4861 aaagagcgcc gtccgaagta acgggcaagg agaacgtgca ttagaacaaa accgccccgc
     4921 gcgctcaatc cgaaaaggat cgagaaaaca aacgctgccg gtgcacttcg cacttgcgtt
     4981 agcaacacca cggcagcagc cgcaagcagc atcgtcgtta cgcctagggt gcggggatgg
     5041 aatctttctg ccagagctcc ccacaagccg ctgccgaatg ctccagttaa ggccatgacg
     5101 ctcagcgctc cagcggctac cgcgggctta atagtcacgt ccgtgaaata cgccaccatg
     5161 tgaaaagcaa tgccgccggt ggaaagcgaa tagaagctgg cagcggcgac gaggagccaa
     5221 agcgtccggg tgcgcatggc atctttcaac gtcca
//
LOCUS       DASUTG010000434         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_659979, whole genome shotgun sequence.
ACCESSION   DASUTG010000434 DASUTG010000000
VERSION     DASUTG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659979"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VF089_09940"
     CDS             complement(<1..1346)
                     /locus_tag="VF089_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6174325.1"
                     /translation="MTLLHPDDASRYVDAYYEALRTRKAFHAEAQVKGRDRKWRWVDT
                     YGTPRFSEDREFLGHVGSCHDVTDMKNAQQALRESEEQCRASFDISTVGMAQGDPITG
                     RLLRVNRRYCEMTGYSEAELLQLCIRDITHPEDRTADFEQFQRLGRGEVSEYWAEKRY
                     LRKDGKVIWVEATANLVRDASGKPTRTVAVIIDITARKQAEAALRESEAQFRTSFELA
                     AVGQAQVNPATGRFERVNKKFCEIVGYSEEELLTMTPADTTHPQDRQAYLETYPRFLR
                     GEIEEYVTEKRDLRKDQTVAWVSIAAKLIRDSDGRPLRTIGVLQDITERKRAEEELKK
                     ANRRKDEFIATLAHELRNPLAPIRNAVEILGLCQPSDADFEWSRNVINQQVKHLASLV
                     DDLIDVSRITRNRLVIKKERVSLTDVINSALDAARLLIEQRGVQLTVSLPIEPVYL"
     gene            complement(1327..1959)
                     /locus_tag="VF089_09945"
     CDS             complement(1327..1959)
                     /locus_tag="VF089_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6174326.1"
                     /translation="MSATQETSLSNAQQLTTTRESDDALRESKLRLQLAVESANIGIY
                     EMNLKNGELSCDERVRAHWGLSAGAAVDYNTFLKAVHPDDREGTQGAIDRALDPNGDG
                     RCRVEFRVIGIHDEIERWIVGTGQLFFDNGQAERLVGTTFDITPRKHAEQRLRESEER
                     FRTLADSSPVPIWVTDAAGGIEFLNAAYCEVLWCHDRGCAETEMGDAASS"
BASE COUNT          579 a          712 c          661 g          661 t
ORIGIN      
        1 ttcaggtaga ctggttctat gggcaacgaa acagtcagct gaactccgcg ctgctcgatc
       61 aaaagtcgcg ctgcatccag agcgctgtta atcacgtcgg tgagagagac gcgctctttt
      121 ttgatcacca gtctatttcg agttatgcgg ctgacgtcga tcagatcatc tacgagtgac
      181 gccaaatgct tcacctgttg attgatcacg ttgcggctcc actcgaagtc ggcatctgat
      241 ggttgacata ggccaagtat ttccacggcg ttgcgaattg gtgcgagcgg atttcttagt
      301 tcatgcgcca gggttgcgat aaattcatcc ttgcgccggt tcgctttttt gagctcctcc
      361 tcggctcgct tacgttccgt gatatcctga agtacgccta tggtccgcag tggcctgcca
      421 tctgaatcgc ggattagttt tgcagcaata ctgacccatg caaccgtttg atcctttcgg
      481 agatcgcgct tttcggttac gtattcttca atttcaccgc gcaaaaaccg cggataagtc
      541 tctaagtatg cctgtcgatc ctgggggtgc gtggtatccg ccggcgtcat ggttaagagt
      601 tcttcttcgc tgtagcctac gatttcacag aattttttat ttaccctctc aaatcggccg
      661 gtcgcaggat tcacttgtgc ctgcccaact gcagccaact cgaagctggt gcggaattga
      721 gcctcgcttt cacgcaaggc cgcttcggct tgcttgcgag ccgtaatgtc gataatcact
      781 gccacggtgc gcgtcggttt accgctagcg tcgcgcacga ggttcgcggt cgcctcaacc
      841 caaataactt ttccgtcttt tcgcaagtag cgcttttcgg cccaatattc cgatacctcg
      901 ccgcgcccta ggcgctggaa ttgttcaaag tcggccgttc gatcttcggg atgggtaatg
      961 tcccgtatgc agagttgcag caactctgcc tcactgtaac ctgtcatttc gcaataacgt
     1021 ctatttaccc gaagcagacg accggttatt ggatcaccct gggccattcc aaccgtggag
     1081 atatcgaacg acgcgcggca ctgttcctcg ctttcacgca gtgcttgttg cgcgtttttc
     1141 atgtcggtta catcatgaca actgcctaca tggccgagaa attctcgatc ttcagagaac
     1201 cgcggagtcc cgtatgtatc cacccaacgc cacttccggt ctctgccctt gacttgcgcc
     1261 tctgcatgga aagctttgcg ggtgcgcagt gcttcataat aggcatcgac atatcgagac
     1321 gcgtcatcag gatgaagcag cgtcacccat ttcggtctcc gcacatcctc gatcgtgaca
     1381 ccaaagaacc tcacaatagg cggcattgag aaactcgata ccaccagcag cgtcagtcac
     1441 ccagatcgga acaggtgaac tgtcggccaa cgtgcggaat cgctcttcgc tctcacgcag
     1501 gcgttgctct gcgtgtttac gaggcgtgat gtcaaacgtt gtgccgacca gccgctccgc
     1561 ctggccattg tcgaagaaga gctgcccagt cccgacaatc caccgctcga tttcgtcatg
     1621 aatgccgata acacgaaatt cgacgcggca gcgtccgtcc ccattgggat ctaacgcccg
     1681 gtcaatggcg ccttgtgtac cctcgcgatc atcaggatgg acagctttta ggaaagtgtt
     1741 gtaatcaacc gccgcgccgg ctgaaagtcc ccagtgcgcc cgtacccttt catcgcacga
     1801 tagctcacca ttcttgaggt tcatttcgta tatgccaata tttgccgatt cgaccgccag
     1861 ctggagccgt aatttgcttt cacgtaaggc atcgtcagac tccctagtcg tagtgagctg
     1921 ctgcgcattg ctgagactcg tctcttgggt cgcagacatg gcggtcctcc ttaataccga
     1981 acaactatac tgtcaccaaa aaatgggacc gtgataccct cgttagagcg gagcgtctgg
     2041 gagtggacga atcgagcagt tgctgcagaa tttatgagtc gcccggagtg agttctacgg
     2101 tgaaaagccg aagcgccgtc gctcaaatca tccgctctgc actttggcga taccacacgg
     2161 gttatgaaga agcgttaggt taaattatgc taatctcttt tgtcaagata atacgggttg
     2221 cgaacataat gaagatatcg ggcaccgact ttcaagatat tgacggcaag ccgcggtctg
     2281 tgtttgcaat ctcaccgcct agagatgagt caactggatc gtgcgattcg cagagcccag
     2341 tcgctgaaat ttgatccttg taaaccgaaa aagctattag taaggggcgg aatccgtgcc
     2401 catcaggttc aacagcaaac gtgctcgatc gggacggcgg ctgttggcag gaaaagcatc
     2461 agcttgaccc acggactgaa actagtttgc cacacttggc cgactatcta ttgcaatgtg
     2521 ttctgaacgg agaatggagc agagaacagc ggtcccgggt ggatacagtg atcaaataaa
     2581 attgttctta tcgcgacgat ttttcagatc agt
//
LOCUS       DASUTG010000435         7400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1697223, whole genome shotgun sequence.
ACCESSION   DASUTG010000435 DASUTG010000000
VERSION     DASUTG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697223"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1512
                     /locus_tag="VF089_09950"
     CDS             265..1512
                     /locus_tag="VF089_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6174327.1"
                     /translation="MNFPSEHDFGLLMFQLFIILTAALSLGELFRRFGQAQVIGEVTA
                     GILLGPSVLGALFPGLFASLFPTSQTHLLGTLAWLGSVFLLLVAGIEVDLAAIRRERR
                     TIFSTSFFSILVPFALGFGFAMGLPDGYLRDPSQRVTFALFVATALSISAIPLIAKIL
                     MDLNLLKTPLGQTVVGCAVINDLVGWMFFAIILTMITGSVGGEMSVMGIALLTLGFSF
                     ITLTAGKHLAQRLFAAFHRRGFPYEGILGLAVLLAFFCAAVTQWIGIHAIFGAFLAGV
                     MIGETGEIINRTRETLRNVSFYVFSPVFFASMGLQANFIANFDWFLVGGTIVLACTGK
                     VSGATLGAVIAGKPRSQALTIGFGLLPQGAMGMILAFLALQYGLISDPIFVALLLAAI
                     ATSVLAGPLIQAMAPTLKRTLKT"
     gene            complement(1790..2194)
                     /locus_tag="VF089_09955"
     CDS             complement(1790..2194)
                     /locus_tag="VF089_09955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6174328.1"
                     /translation="MIKREVLIALAGGPSHGKGMVQGIKAGGFIFFSAIRGNHPVTNY
                     ESSDTKEQARQAFENLKVLVGAAGAGLEHVVQVTLYLHDLRYRLPFHEIRMEYFPKDP
                     PARIAIQVADANAQPGRNAYFALDVIALDPNA"
     gene            2887..3771
                     /locus_tag="VF089_09960"
     CDS             2887..3771
                     /locus_tag="VF089_09960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174329.1"
                     /translation="MIPLWPAEDVDPARVQLRLEIIQYGGLGLALIAACFFIWKTPSK
                     PRKIRIAILTICILGLGISSAVRGNPEIIRRSGDSQIAQSPKYRFSEDWVTDNSANWE
                     RVLSAFKGKANIHALEIGSFEGRSALWFLENVLTDPTASITCVDIWAGGYEKLFDENI
                     KAYGQPNKLVKIKSRSDEALRELNRDSFDFIYIDGSHLAKDVLVDAVLAWDLLKPGGY
                     IIFDDYNWYGPRSWLVPNFTPKIAVDAFVDIFDPYLEVIHKDYQLVVRKTMNPDNVAL
                     DTYKQLRPVLIEVQQALR"
     gene            complement(4123..5028)
                     /locus_tag="VF089_09965"
     CDS             complement(4123..5028)
                     /locus_tag="VF089_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174330.1"
                     /translation="MKTLRLETTAPFQGLAELVAYDEGLFEKEGIQIEWMDREKGLDK
                     SPQLHITHHKDASRYTSHGRMMESGQADLYNACEWGNFSRVETTNVRSRQVGRRSMVN
                     FAALVVRADSPVQVPQQFANVQIGVPFYSGTHYLCLQMLEGFVPRELIKLGRVPNGSN
                     YRFKMLMDKTIEATTLTEPYISLAEKMGCRLVISAFHHGTDVASERVDAETYAAFNRA
                     ICEAVRRIMANKRAYMHYFIDYYRDKDPMISQLTIDDLRESRLYLVDPAPIPADELQR
                     TYDWMKSWDFLETAPCATDLVDMSV"
     gene            5547..6074
                     /locus_tag="VF089_09970"
     CDS             5547..6074
                     /locus_tag="VF089_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX6174331.1"
                     /translation="MGAEETKHDLWLKRINTWIAILGGSVASVAGTYSYLPSFFPPAP
                     GHIAAVVREQGGKPVPRAHVELLSPDNVLLAASETDRNGRFIKKDLEAGSYIVKISRA
                     AFEPQTAKVSIASKKTTDLDLVLRSRPRAQVPNSPQLNSPQTRAISQPEGNAIRSALE
                     ETGAAWIKNLSNPGR"
     gene            6546..7301
                     /locus_tag="VF089_09975"
     CDS             6546..7301
                     /locus_tag="VF089_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6174332.1"
                     /translation="MRQAQQISRRTWLARVALGSFAVWSELHFGFGRRGWGVAIGGSD
                     LALGVAHAQNLEPAKALRVNLGIVNAYVLIRGKEAAVVDTGTPNNGSKIAEVVRSAGL
                     DWNGVHHVILTHYHPDHIGSIGEVLTAAEKATAYAGSADIPQIKSPRPLKAVNDNDEV
                     FGLRVIATPGHTPGHVCVFDPAGSVLILGDAMNNIGNKLAGPNPQYTADMAQAHQSVK
                     KLANLKFQRAVFGHGEPIENNASQAIAKLAGTL"
BASE COUNT         1692 a         2001 c         1932 g         1775 t
ORIGIN      
        1 cgttttacga acgcgatgag aagtaatccc gcacaagcga gctgccaaag tacgctaaca
       61 atcgcgttcc ggtcttgtag actaaaagga tgctgaaaaa aacctcttgc aggctgctca
      121 aaaagatcca gaggcgaggc gcgcgaaaaa tcgacgagcg gaggcgtact tacagtacgt
      181 tgcagcgagg tgattgagcg gcaacgaagc atatgggttt tttcagcagc ctgctagaag
      241 aatcgaatcc actgaaaggt tgtcatgaac ttcccatcgg agcacgactt tggtctgctg
      301 atgtttcagc ttttcatcat cttgacggcc gcgctaagcc tgggcgaact gtttcggcga
      361 ttcggtcaag cgcaggtcat cggtgaagtc accgccggca tcctgctcgg cccttcagtt
      421 ttgggcgcgc ttttccccgg gctattcgcc tctttgttcc caacttctca aactcacctg
      481 ttgggcacac tggcgtggct cggcagtgtt tttctgctct tggttgccgg tatcgaagtt
      541 gatctggctg cgattcgccg cgaaaggcga actattttct cgacaagttt cttcagcata
      601 ttggtgccgt tcgcactggg attcggcttc gccatggggc ttccggacgg ctacctgaga
      661 gatccttcgc aacgggttac tttcgctctt tttgttgcca cggcgctctc gatctcggca
      721 attcctctga ttgcgaagat tctgatggac ttaaatctcc tgaagacacc gttgggacaa
      781 accgtggtgg gctgcgcggt aattaatgat ctcgtcggat ggatgttttt cgcgatcatc
      841 ctgacaatga tcaccggaag cgtcggcggt gagatgtcgg ttatgggaat tgcgctcctc
      901 actttgggtt tcagctttat caccctgaca gccggaaaac acctggccca gcgtcttttt
      961 gcagcttttc atcgccgcgg ctttccttac gagggtattt tgggattggc tgtcctgttg
     1021 gcattttttt gcgccgcggt gacgcagtgg atcggcattc acgctatctt cggcgccttt
     1081 ctggccggcg ttatgattgg cgagaccggc gagatcatca atcggacgcg agagacgttg
     1141 cgcaatgttt ccttctacgt cttctcgccc gtgttcttcg cttcgatggg tttacaggct
     1201 aactttatcg ccaatttcga ctggttcctg gtcggcggca cgattgtcct ggcctgtacc
     1261 ggcaaagtga gcggcgccac ccttggcgcg gttatcgccg gaaagccgcg tagtcaggcg
     1321 ctgaccatcg gcttcgggct tctgccacag ggagccatgg ggatgattct ggcctttttg
     1381 gccttacaat acggcttaat cagtgacccg atatttgttg cgctgctgct tgcggccatc
     1441 gctacttccg tacttgcagg accgctcatt caggctatgg caccgacgct caagcggacg
     1501 ctgaaaacat gaggaaaaca atactcgcat ggttcacagc cgctaacctg tggaagtttt
     1561 tcccattact agtgatctgg aacaatcacg tcagacaagt cttccggata tcgagactcc
     1621 gctttatctg atcaatcatc acctggttaa gctttactag acagttcagt gcctgaccaa
     1681 gaattcgcat aatccggatt acaactgtgc ggcgacctct cgcggggtgg agtctttcgt
     1741 gccccggcgg aaacgtcttg gagcgattcc acattatctt tggttcggat tatgcgttgg
     1801 gatcgagcgc aatcacatcc agagcaaaat acgcattgcg acccggctgg gcgttggcgt
     1861 cggccacttg aattgcgata cgtgccggcg ggtccttcgg aaagtattcc atccgaatct
     1921 cgtgaaaggg taggcgatat ctgagatcgt gcagatagag cgtcacttga acgacatgct
     1981 ccaggccagc acccgccgct ccgaccagaa ctttcaagtt ttcaaaagcc tggcgcgctt
     2041 gttccttggt gtcgctgctt tcataattgg ttacgggatg gttgccgcgg attgcagaaa
     2101 aaaagataaa gccgccggct ttgatccctt gcaccatccc ttttccatgc gacggtccgc
     2161 ctgcgagggc gatgagaact tctcgcttga tcatcgattg atgttctcct cgcatcgaaa
     2221 tttccgtgaa cttctaccat ggctttgcca atacgaacaa tcgccagatt tctcactagg
     2281 tatacacctg gtctggtgac atcttcggac atcgtggaat acgatcttga ttcgaacgcc
     2341 ctagacggcc agggccaact ccgttctcga atctttcaat acacggccaa acctacaaag
     2401 cagagtccta cgaaacgctc gcacatcgaa cagaagactg cgcggacgga ctctagccgt
     2461 aatcagcgta cccgtgacac cagcacccca cggcggccgc atctgccgcc tgctctgatg
     2521 aggcccgaca catgcttgag cagcggcacg ctttggatca cttgagtagc gccatcgaca
     2581 cggctttgat ctgcgcattg cttgccgtta cccgggaaag agccgcgacg tttggattcc
     2641 actaatcacc tgaaagaagc agcccgcgcc gatgatgttt acatatctca acagtcgagg
     2701 ttgagattac aattttcaat tgacaactcg gtgtcaatgc caggaaaata tgcagctact
     2761 ggacccacgc cctaaaacta ttattcggta cacttaagga agaggtgact tattaatctg
     2821 cttatcgcta gctgcgcaac cgtcggcggt gcttccgtcg cccttcttgc aactttggcg
     2881 cgctacttga ttcctctgtg gcccgctgaa gatgttgatc cagcaagggt tcaacttcgc
     2941 ctcgaaatca ttcagtatgg cggccttggc ctggctttaa tcgcagcttg cttctttatc
     3001 tggaagacgc cttccaaacc acgaaaaatt cggatcgcta tactcacaat ttgtattctg
     3061 gggttgggca tttcttcagc ggttcggggg aatcccgaga tcatccgcag aagcggggat
     3121 tcacagattg ctcaatctcc caaatatcga ttttccgaag actgggttac ggataattcc
     3181 gccaattggg agcgggtact tagcgctttc aaaggaaaag cgaatattca cgcgttggag
     3241 ataggcagtt ttgaaggccg ttctgcgctc tggtttttgg aaaacgtact aacggatcct
     3301 accgcttcca ttacctgtgt tgatatttgg gcgggtgggt atgaaaaact ttttgatgag
     3361 aatatcaaag catacggaca gcccaataag cttgtcaaaa tcaaaagtcg gtcggacgaa
     3421 gcattacgcg agttaaatcg tgacagcttc gatttcattt acatagatgg gtctcatctg
     3481 gcaaaggacg tgctcgtcga tgccgtcttg gcgtgggacc ttttgaaacc gggcggatac
     3541 attatcttcg acgattacaa ttggtatggg cctcggtcat ggctcgtccc gaatttcacg
     3601 cctaagatcg cggtcgacgc attcgtggac atcttcgacc catatctgga agtgatacac
     3661 aaagactatc agctagtagt tcgaaagacg atgaaccccg acaacgtcgc actcgacacc
     3721 tacaaacagc ttcgccccgt tctcattgag gtccagcaag cgcttagatg agatagggag
     3781 tccggcgatt cgcggatgag tagtaagatc tccgcctcta gttcctggga aaacgatcca
     3841 agagggcggt tatatcaata tggcaagcat catcagttca tggtgccgcc cattagccgc
     3901 tctcccagag ctagtaaact gagggaatcc tgaggctaat cagaatccaa aagacctaaa
     3961 gcgcactatg gtcctgccac tcattcgaga aacgtatgaa gacgctcagt cccagcgtgc
     4021 aatacgcgaa cggcgcgtgc tctccaacca cgatggcgac aactatccga tttcccttct
     4081 caaatgtgcg aaacctggat ctccggcccg gccctttgcc ggctatacgc tcatatcaac
     4141 aagatcggtc gcgcaggggg cggtctcgag aaaatcccag ctcttcatcc agtcgtaggt
     4201 gcgttggagt tcatcggccg ggatcggtgc gggatcgact agataaaggc ggctctcgcg
     4261 cagatcgtcg atcgtgagct ggctgatcat cggatccttg tcccgatagt agtcgataaa
     4321 gtaatgcata taggcgcgct tgttcgccat gatacgccgg acggcttcgc aaatcgcgcg
     4381 attgaaggcg gcgtaagttt cggcatcgac cctttcggac gctacgtcgg tgccatggtg
     4441 gaaagccgag atgacgaggc gacagcccat tttttcagcc aacgaaatat acggttcggt
     4501 caaggtggtc gcttcgatgg tcttgtccat gagcatcttg aaacggtaat tggacccgtt
     4561 cggcacgcgg cccagcttaa taagctcccg cggcacgaag ccttcgagca tctgcaagca
     4621 cagatagtgg gtgccgctat aaaacggcac accgatctgc acgttggcga attgctgagg
     4681 cacctggacc ggcgaatctg ctcgaaccac aagcgcggca aaattcacca tggaacgacg
     4741 gccaacttgc cggctgcgga cgttagtggt ttcaacccga gagaaattgc cccattcgca
     4801 agcgttgtag aggtcggcct gacctgactc catcatgcgg ccatggctgg tatagcgcga
     4861 ggcgtcttta tgatgggtga tgtgaagctg cgggcttttg tccaggccct tttcacgatc
     4921 catccactcg atctggatgc cttccttttc gaacagaccc tcgtcatagg ccaccagctc
     4981 ggccaatcct tggaatggcg cagtggtttc caatcgcaat gtcttcatag attcctctcc
     5041 atcggcgtta ggtaagttat cctctataca atggctggcg atctaacttc tgtcaaacgc
     5101 ccatcctgag aaagctcatc gcgatccggc tgggcgcata acatattgcc aatccagtgg
     5161 ccgccgcgaa actagcaatt cctcgccaag ctgtcgaatc gaaaacaaac agaccgaaat
     5221 tgacgagcat cgcttagaag gtcagcaagc gcaccgatcg agcagtgaag cgctagtgtt
     5281 gctagccagc ggttttgatc gccaagcacc ttcggttgaa cgccgattgc catgcgccga
     5341 taatgtgctc gaaaattgac cagcagtttg ctgttacaaa cttctatttg cgcgtaactt
     5401 cttccgctgc gaagctgacg tttttgtctg gattcttctt tgtcatactc tcgtttccgt
     5461 gcaggcaacg tctcctgccc tggcatatcg cttgctcaaa gcagttcccc gcaaggctat
     5521 caaagatcaa agacaaagag gttgccatgg gagctgaaga aaccaaacac gatttatggc
     5581 taaaaagaat taatacgtgg atagccattc ttggcggctc agtcgcttcg gttgcgggga
     5641 cctatagtta tcttccctcg ttttttccgc ctgctccggg ccacatcgcg gctgtcgtcc
     5701 gggagcaagg tgggaaacct gtcccgcgcg cccatgtgga gcttttaagc ccggataatg
     5761 ttttgctcgc tgcttctgag acggatcgca acggacgctt tatcaaaaaa gaccttgaag
     5821 ccggcagtta cattgtaaag ataagcaggg cggcgtttga gccgcagact gccaaagtga
     5881 gcatcgcttc caaaaagacc acggatttgg atttggtttt aagatcgcga ccccgcgctc
     5941 aagttcccaa cagtccccaa ctgaacagtc ctcaaaccag agccatttcg cagcccgagg
     6001 gcaatgccat tcgatcggcg ctcgaagaga ccggcgcagc ctggatcaag aacctcagca
     6061 atcccggaag ataaacacaa actccgaagt acggtttgca ttgttgtcat tgctgagctt
     6121 ctcacaagtt tagaactgtt cctcctcatt gcactctttt tttcttcagg gaagatccaa
     6181 cacagacaag tttgacgatt gaaagtttgc cagcggcaac gattatctgc cttcccggtt
     6241 gaatcgaatc gaacatcttg cgagataatc ctcctgtact agagaacgat tcctcgcggt
     6301 tcccccgaga tttgcagccc ggatacaaga tctaagaacc aataacggtt catggttgag
     6361 cgaacgctca gcctgatggt tcggacgcag aatgatcgac cactgcattt acgaaaacta
     6421 tgccatcgca gcggcaacaa tcagtgcgca ttaacgaggc tggattgtag gctctatttc
     6481 ctgtaacgat ttgaactaag cgcaggggac gagttgaaca aattccttta acgctggagg
     6541 tggcaatgcg gcaagctcag caaatcagtc gtcgaacgtg gttggcccgg gtcgcgttgg
     6601 gcagtttcgc agtatggagt gagcttcatt tcggcttcgg ccgcagaggt tggggcgtcg
     6661 ctatcggtgg tagtgatctc gcgcttggcg tcgctcacgc tcagaacctg gagccggcaa
     6721 aagcccttcg ggtcaacctg ggcattgtca acgcctacgt ccttatccgc ggcaaggagg
     6781 cggcggtcgt cgacaccggc acacccaata atggatcaaa gatcgccgag gtcgttcgca
     6841 gcgccggact ggattggaat ggggttcacc acgttatcct gacacactac catcccgatc
     6901 atatcggcag cattggcgag gtactaacgg cggctgagaa agcgacggcg tacgctggct
     6961 ccgccgacat cccgcagatc aagtcaccgc gcccgctgaa ggcggtcaat gataacgatg
     7021 aagtgttcgg cctgcgcgta atcgcgacac ccggtcacac accaggacat gtgtgcgtat
     7081 tcgatcccgc aggatcggtg ctcatcctgg gcgatgccat gaacaacatc ggcaataagc
     7141 tcgctggtcc caatccccaa tacaccgccg acatggccca ggcgcaccag agtgtgaaga
     7201 aactggcgaa cctcaaattt cagcgcgcgg tcttcggcca cggggagccg atcgagaaca
     7261 acgcgtcaca ggcgatcgcc aagctcgctg gcactctcta aggcatccgc cgagaccgct
     7321 gcgattagtc cttgagctat cagtaccact cagccactca atgaaaactg agccggatgc
     7381 cgacaggcga gttcgcctct
//
LOCUS       DASUTG010000436         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1401448, whole genome shotgun sequence.
ACCESSION   DASUTG010000436 DASUTG010000000
VERSION     DASUTG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401448"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..423)
                     /locus_tag="VF089_09980"
     CDS             complement(82..423)
                     /locus_tag="VF089_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174333.1"
                     /translation="MTRVNFDPELGEHRFYFLSCIIILIIRYMPTGQASSAKELSAPL
                     AIAPRENPKTAIAAKRSSGAAQQIAMPIGPLTQLFPCIGQAADPCSGLTAGAVDFPQS
                     PFAPPQRFDQT"
     gene            721..1134
                     /locus_tag="VF089_09985"
     CDS             721..1134
                     /locus_tag="VF089_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6174334.1"
                     /translation="MDISRNMLNVARQRAAKLSMHVSFSLADAEVLPFADHSFDTVVS
                     SLSTCTLPNPVGVLKEMARVCRTGGKILLLEHGRSDHEWLGHWQDRHADKFAEPLGCH
                     WNREPLELVEQGGLKVVRAHRTFLGIFHQIEAQTI"
     gene            1799..2374
                     /locus_tag="VF089_09990"
     CDS             1799..2374
                     /locus_tag="VF089_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX6174335.1"
                     /translation="MLVWIGEIWNFFEAVVALWSGIGAGSVALIGFGFDSILELAAGG
                     ILIWRLQTKWENSEEESSGERKAHKLVGITFFVLAVYILIHSVATLFGVFREPEQTLV
                     GIMLIIASAFVMTALYFQKITIADKIGSRALKAEAKETLVCDLQDLTVLVGLAANFLL
                     GWWWADPVAALGLIPFLIKEGLEGMAGEDDE"
     gene            complement(2772..3329)
                     /locus_tag="VF089_09995"
     CDS             complement(2772..3329)
                     /locus_tag="VF089_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6174336.1"
                     /translation="MKKVFLSLVSWALISSLAGVATVAAAPRQGASEVQGSAGFFHAE
                     DSDFGNLNLDVSYGVYLSPGWQLGFRQALNYLFVDDASDSWIATTAPFINYHFRVTDI
                     VYPYLGAFIGAAWNDDDVTGTLGPQLGVKLFVHEQTFVNLGYRYEWFWDKFERINNES
                     DNGNHIFNIGIGFTWGGSGAGTRRP"
     gene            3595..4380
                     /gene="lgt"
                     /locus_tag="VF089_10000"
     CDS             3595..4380
                     /gene="lgt"
                     /locus_tag="VF089_10000"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX6174337.1"
                     /translation="MDYLIWNVDPEIFRLGRIAPHWYGLFFALGFFAGYFILLKIYRN
                     ERRREENLSDLFLYIFLGTLIGARLGHVLFYQPDYYLVRPWEILMIWQGGLASHGGFT
                     GVLIALYFYCKKYTDMSFLELSDRLAIAALPAASLIRVGNFFNSEVLGVPTNLPWAVI
                     FIRVDPIPRHPAMLYESLSYLFIFSAVYLAYWKTGLIKTSGRVLGLVLALSFTARFLV
                     EFLKEAQVPFEQGMLLNMGQLLSIPFIVAGLVLLYLPRKSGRR"
BASE COUNT         1105 a         1041 c         1186 g         1106 t
ORIGIN      
        1 aggatgaacg aaatgatggg ccgataagtc tgaaatatag ggagcaatag ccaaaaattg
       61 ggttcaagca aaaggagcat tctatgtctg gtcaaatcgt tgcggaggag caaaaggtga
      121 ttgcggaaaa tcaactgcac ctgcggtcaa acctgaacac ggatcagccg cttgcccgat
      181 gcagggaaaa agctgggtta agggaccgat tggcatggcg atctgctgcg cggccccgct
      241 gcttcttttc gccgcgattg cggttttcgg attttctcta ggagcgatcg cgagcggggc
      301 gctgagttct ttggcgctac tggcttgtcc ggtcggcatg tatcttatga tgaggatgat
      361 gatgcaagat aaaaagtaga atcgatgttc tccaagctct ggatcaaaat taactcttgt
      421 cattgcttgt ggagtggcaa gttacaacag catacaagct gacaacaacg cagaaagaca
      481 aaacgacgct ctgaaaacgt ttaggatttt aatggctgta gaatttgcgt cacgagagat
      541 atctgaaaaa tacgatagat tcgctccatg gtacgactgg cttgaaggca ttccagatct
      601 gctgggaatc agaaggcttc gccgcagctt attctcgcgt gcttcaggaa gcgtcctaga
      661 agttgccgta ggtaccggga aaaattttcc ttatcatcct catgatgccg gactacggcc
      721 gtggatatca gcaggaatat gctaaatgtt gcgcgtcaac gagccgcaaa actatccatg
      781 catgtttcct tctctttagc ggatgcggaa gtgctgcctt ttgccgacca ctcttttgat
      841 actgtcgttt cttccctcag cacttgcaca cttccaaatc cggttggcgt cttaaaagag
      901 atggcgcgcg tttgtcggac gggcggtaag atcttgttac tggagcacgg ccgaagcgat
      961 cacgagtggc tgggacattg gcaggaccgg catgctgaca aatttgccga gccgcttggc
     1021 tgccactgga acagagagcc attagagcta gtcgaacagg ggggcttgaa agttgttaga
     1081 gcgcaccgaa ccttcttggg gatttttcac cagattgagg cgcaaacgat ctgattctcg
     1141 atcatcccag aactgcctta gcattcgttg aatgagatct catttaacga gagtaacgat
     1201 ggagagttca catgaatcga cgcaatttcc tcatcagagg cttgctcgca gtaggttgta
     1261 tgacagttgt cggctgccaa tggttccgcg cgacggagca ccgctataaa ttagcgccag
     1321 agaatatttt gccgccgaac atcgcgaagg ctcctgaaga agtccgcgaa gcatatcggt
     1381 tcgcgattat taatcgcgac acgctccgct acatcccgtg ttactgcgct gcggatcaga
     1441 gggccacacg agcaatgcct cgtgttacat caaagacagc tcaaccggcg acaaattaga
     1501 attcggtcga atgagccttg cttgaggcat ctgcataggc atcacgcgtg acgtgatgaa
     1561 gatgatggat gaaggcaaaa gtctaaaaga gatgcgaacg cagattgaga aaacttacag
     1621 tcactatggg ccgccgaccc cgacgccgcc cgttccgtag tgttattgca ggtgagcggc
     1681 ctagatcagg tcccttggtg atgcccctcc atcgcgaccg tacgattcag tagtagcgta
     1741 tcgagacaaa aatatgcacg cgagcacagg cttagatggt ctcaagaaga aggctttgtt
     1801 gctggtttgg atcggcgaga tctggaattt cttcgaggcc gtagtagcct tgtggtctgg
     1861 aatcggagcc ggtagcgttg ctctcattgg ttttggtttt gacagcatcc tggaattggc
     1921 tgcgggtggt attctaattt ggcgtctcca gacaaagtgg gaaaatagcg aagaggaaag
     1981 ctcgggggaa aggaaagctc acaaactggt cggaataacg tttttcgtat tggccgtcta
     2041 tatcttgatt cattccgtcg cgacactctt cggtgttttt cgtgagcctg aacagacctt
     2101 agtggggatc atgctgatta tcgccagtgc gttcgtcatg acagcccttt attttcagaa
     2161 aatcactatt gcagacaaaa tcggttctcg tgctttgaaa gcggaggcaa aggaaacact
     2221 ggtctgtgat ttacaggatc tcacggtgtt agtcggcctt gcagctaatt tccttcttgg
     2281 gtggtggtgg gcggatccgg tagctgcttt gggattgata cctttcttga ttaaagaagg
     2341 tctcgaggga atggcgggag aggacgacga gtagtaaatg gaccttcgct gccgcgattg
     2401 cggtgtgcca taaagcaacc cctgttctgg aacaattatg tactggtcgt aaaacttcta
     2461 aggagatcga gacaagccga agggttagct gtctacggat tcatacacaa accaaagaat
     2521 ctgcgatcct tccttggcac aagcatcctg gccgaaacag ccgctatcat agcgccagac
     2581 ctggacgccg aacagataaa aaactcgttc ggtgcagtct cggcgatgcc ggaggaactc
     2641 acaaaactcg ggaagggcac tcacttagga cagcaatttg aggtgacaaa cttctcaccc
     2701 aactcgccgt ggatgacgat cagggtgaga agttgataac gtgacgctgg attattgact
     2761 agcagccagg tttacggccg tcgagtgccg gctccggaac cgccccacgt aaagccaata
     2821 ccgatattga atatgtggtt gccgttgtcg gattcattat tgatacgctc gaatttatcc
     2881 caaaaccact cgtagcgata tccgagattc acgaaggtct gttcatgaac gaaaagtttg
     2941 acaccgagct gagggccaag ggttccagtc acgtcatcat cgttccacgc tgcgccaatg
     3001 aaagcgccga gataaggata aacgatgtcg gtcactcgaa aatggtaatt aatgaaaggt
     3061 gcggtagtgg cgatccagct atcactggca tcgtctacga agagataatt gagcgcctgt
     3121 cgaaagccta gttgccatcc tgggctcagg taaaccccat agctcacatc gaggtttaaa
     3181 ttgccgaagt cagaatcttc ggcatgaaaa aatccagccg atccttgaac ctcacttgcg
     3241 ccctgtcgag gtgcggcagc gacagtcgca actccagcga gcgaactgat cagcgcccaa
     3301 ctcactaaag acaggaaaac tttcttcata gaatacctcc actcgtaaaa ttgaaacctg
     3361 taatgtcaga aacatgccat agtcggtcga aggacgcaac aaaaaaacaa gttccgccag
     3421 gatgccggag cagacccgca cacggaaaag cctattccgt gctgaggctc tcgaggatgt
     3481 gtggcacggc gggagcaaaa ccgccgctcg agacggttgg ctgtcgatga atgagacagg
     3541 acgcgttcat caatgccaca gcgggataat cagagaacaa gtccagggaa gagagtggat
     3601 tatctaatct ggaacgtaga tccggaaatc tttcggttgg gtcgcatcgc gccccattgg
     3661 tacgggcttt ttttcgcgct cgggtttttc gcgggctatt tcatccttct taaaatttat
     3721 cggaatgaga gaaggcggga agaaaatctc tcggatcttt tcttgtacat tttcttgggc
     3781 acgttaatcg gcgccaggct cggacacgtg ctcttttacc aacctgacta ttacctggtt
     3841 cgcccgtggg aaatcttgat gatctggcaa ggcggtttgg cgagccacgg cggttttacc
     3901 ggagtgttga ttgcgctgta tttctattgc aagaagtaca cggatatgag ctttctcgag
     3961 ctcagcgatc gtttggcgat cgctgcgctg ccggcggcga gcctgattcg ggttggaaac
     4021 tttttcaact cggaagttct tggtgttccg acaaaccttc cgtgggcggt tatattcatc
     4081 agggtcgatc ctattccacg gcatccggcg atgctctatg aatcgttatc atacctgttt
     4141 atcttttccg cagtttacct tgcgtattgg aagacaggcc tcataaaaac ttctggtcgt
     4201 gttcttggtt tagtccttgc gctctctttt accgcacgat tcctcgtcga atttctcaaa
     4261 gaggcgcagg ttccctttga acaaggcatg ctcctcaaca tgggacaact actgagcatc
     4321 ccctttatcg tagcagggct ggttttactt tacctaccgc ggaaatctgg tcgtcgctga
     4381 ccgctctatc ttgaatgcga agagttttgt aggatggctt ctctttacgg ttatgttg
//
LOCUS       DASUTG010000437         6457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_67506, whole genome shotgun sequence.
ACCESSION   DASUTG010000437 DASUTG010000000
VERSION     DASUTG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67506"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="frc"
                     /locus_tag="VF089_10005"
     CDS             complement(<1..1146)
                     /gene="frc"
                     /locus_tag="VF089_10005"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX6174338.1"
                     /translation="MSKALDGIRILDMTHVQSGPTCTQILAWFGADVIKVELPGRGDI
                     TRGQLRDKPNVDSLYFTMLNCNKRSITLNTKNEKGKEIFRQLIKACDVMVENFAPGAL
                     DRQGFTWEVIRELNPRMIYASVKGFGPGPYEDCKVYENVAQCTGGAASTTGFDDGPPL
                     VTGAQIGDSGTGIHLVAGILAALFQREKTGQGQRVTCAMQDTVLNLCRVKLRDQQRLA
                     AGPLMEYPQYPNGVFGDAVPRAGNASGGGQPGWAVRTKGGGPNDYIYVIIQPPGWEHL
                     MKLCGRPELIADPEWATPDKRLPKLGKCFELIEQWTMTLDKLEVMRKLNEFDVPCGPI
                     LDMREIAEEKSLRDTGTVVEVEHPKRGKFLTVGNPIKLSDSPTEVRTS"
     gene            complement(1274..2986)
                     /locus_tag="VF089_10010"
     CDS             complement(1274..2986)
                     /locus_tag="VF089_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6174339.1"
                     /translation="MAENSESKKEATGPVISGGHLVAKALKNEGVEVIWTLCGGHIID
                     IYDGCIDEGIKIIDVRHEQVAAHAADGYSRQSGKIGCVVTTAGPGTTNAMTGIANAFR
                     AESPLLMIGGQGALSQHKMGSLQDLPHVDFMSPITKFAASVPHTERIADMVSMAFRES
                     VNGAPGPSYLEIPRDVLDAKVPLERARVPKPGAYRASTKSVGDPADIERLADILVKAE
                     RPAALFGTQVWTCRGHDVAVEFCRTLNIPGYMNGSARGIFAPGDPHGFNRTRRLAFDK
                     ADVILIVGSPFDFRLGYGRRLRADATVVQIDLDYRNVGKNRDISLGLVGDCGAILNAV
                     AQAASGRASNGNAKRAEWMKELRSAEGKAYEKLLPSFKSESTPINPYRVAYEINEFLT
                     DNTVYIGDGGDVVTITATAVEPRKAGNWMDPGPLGTLGVGTSFAMATKFVHPKKEVLC
                     YYGDGSFGMTSWDMETAQRFGLPYLAVIGNNSAMNQIRCGQIGKYGPERGCVGNKLGD
                     VDFEKFAECFGGWGIAVREPAQIRPALEKARERVAGGQCAIVNIWVDINEYAPGTKAQ
                     TMYK"
     gene            complement(3088..3852)
                     /locus_tag="VF089_10015"
     CDS             complement(3088..3852)
                     /locus_tag="VF089_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX6174340.1"
                     /translation="MLRAIKRIRIHEEVFNQIRQLIEEGRFRARDQLPSERELAETFK
                     VSRTSVREALRALETQGLIVSRTGTGNFVADLPVESLIGPLARLLIDEKKALVDMFEM
                     RKLIEPHIAALAAERARRDDVAQLKRIVAKQSEAVGRGETGVEADAELHFFISRTTRN
                     QALQKLVSGLMKLLSRSREESLQTHNRRASSIAAHRRIVAAIEKHDKAKARTEMLRHI
                     EQVEESVLESGRLSKSYGQNSLRDREVLRDQARRIG"
     gene            4080..5795
                     /locus_tag="VF089_10020"
     CDS             4080..5795
                     /locus_tag="VF089_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6174341.1"
                     /translation="MPDADLTRLDSILTRLAPQGRAALLPALHLAQQLHGWIPEAVAA
                     EVGRILGVPLADVHGVIDFYEMLSRQPIGRTVVRVCNAPVCGLAGADGLADALCGYLK
                     VKPGQVSADGEFTIEHAPCLGLCDHAPALLTGDSAIGRAAPEQADTICARKVEKPISF
                     VGGDIRILTSSCGQGHSTTFAEYSSGGGYAALKKALTITPQDVIAEVKAAGLVGRGGA
                     AFPTGIKWEGAAKAVGQPKYIVCNADESEPGTFKDRILMEEDPHRTIEGMIIAAHAVG
                     ARYGYIYIRGEYPCAFKAMNDAVDEARRSGIIGENILGSGLDFDIEMRLGAGAYICGE
                     ETALLESIEGKRGFPRIKPPFPTTHGLFGKPTVINNVETFCNIPLIIEGGAAEYRKIG
                     TQRSPGPKLFCVSGQIQEPGLYEVPFGVTLRHLLFELAGGLKQGRKLQAVLMGGAAGA
                     FASEKDLDVILSFENLSAAGLPLGSGAVMVFDNTADLRDVLKRLARFFADESCGKCYP
                     CQLGTQRQHEILDRAAAGVAVAGDRERLSDVGGTMIDASLCGLGQTAPTAVLSAMKLW
                     PDLFK"
     gene            5892..>6457
                     /locus_tag="VF089_10025"
     CDS             5892..>6457
                     /locus_tag="VF089_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6174342.1"
                     /translation="MADTTLTIDGESVTVPDGTTILKAAEKTGRQIPTICYHDHCTAN
                     GLCRICVVEVDGVKPLVPACLSRVSKGMKVVTSSARAERSRRTILEMLASAVDLSEAP
                     EIQKFIDSYGADTTRFPDAERREYPVLDDNPMYLRDYSKCILCWRCVQVCAEDAQYTY
                     ALNFKGRGFDTGIATFFDKPMPETTCVF"
BASE COUNT         1270 a         1916 c         1848 g         1423 t
ORIGIN      
        1 ggacgttctc acctcggtcg gcgagtcgga cagttttatc ggatttccca ccgtcagaaa
       61 tttgccgcgc ttgggatgct ccacttcgac gacagtgccg gtgtcacgca gcgatttttc
      121 ctccgcgatc tcgcgcatat cgagaatcgg cccacatggc acgtcgaatt cattgagctt
      181 gcgcatgact tccaatttat cgagagtcat cgtccactgc tcgatgagct caaagcattt
      241 accgagcttg ggcaggcgct tgtccggtgt cgcccattca ggatcggcga ttagctcggg
      301 ccggccgcag agcttcatca ggtgttccca tccaggcggt tgaatgatga cgtaaatata
      361 gtcgttcggt ccgccccctt tggtgcgcac cgcccagccc ggttgtccgc cgcccgatgc
      421 gttacctgcg cgcggcacgg catcgccgaa gactccattg ggatactgcg ggtattccat
      481 caggggtccg gctgcgagcc gttgctggtc acgcagtttg acccggcaaa gattgagcac
      541 ggtgtcctgc atggcgcatg tcacccgctg cccttgaccg gttttttcgc gctggaaaag
      601 cgccgcgagg atcccggcaa ccagatggat cccggtgccc gagtcgccga tctgcgcgcc
      661 cgtgaccagc ggcgggccat cgtcgaaccc ggtcgtgctc gctgcgccgc cggtgcactg
      721 ggcgacgttt tcatagacct tgcaatcttc ataaggccct ggaccgaagc ccttcacgga
      781 agcatagatc atgcgcggat tgagctcgcg aatgacttcc caggtaaatc cttgacggtc
      841 gagcgcaccg ggggcgaagt tctccaccat cacgtcgcac gcctttatga gttggcggaa
      901 aatctctttg cccttctcat ttttggtgtt gagcgtgata cttcgcttgt tgcagttgag
      961 catggtgaaa tagaggctgt cgacgttcgg cttgtcgcgc aattggccac gggtgatatc
     1021 gccgcgtccg ggcagttcca ctttgatcac atcggcgccg aaccaggcca aaatttgtgt
     1081 gcaggtcggc cccgactgca catgcgtcat atcgagaata cgaattccgt cgagcgcttt
     1141 gctcatcgct tacctttctg cgaagatttt gctttcacca cgaaagacat gaagatcacg
     1201 aagttcggag atctaataac tttaaccctt cgtgatcctc gtggtgagat ctctctttac
     1261 gttcggctgt gtcttactta tacatcgtct gcgctttggt ccccggcgca tattcattga
     1321 tatcgaccca gatgttgacg atggcgcatt ggccgccggc gacccgctcg cgcgcttttt
     1381 cgagtgccgg gcgaatctga gcgggctcgc gcacggcaat gccccagccg ccgaagcatt
     1441 cggcgaactt ctcaaaatcg acgtcgccaa gtttgttgcc gacacagccg cgctcgggtc
     1501 cgtacttgcc gatctggccg cagcggattt gattcatcgc cgagttgttg ccgataaccg
     1561 cgagatacgg taggccgaag cgctgcgctg tttccatatc ccacgatgtc atgccgaaag
     1621 agccgtcgcc gtaatagcac agcacttctt tcttgggatg gacgaacttg gtcgccatcg
     1681 cgaacgaagt gcccaccccg agcgtgccga gcggcccggg atccatccaa ttgccggctt
     1741 tgcgcggttc caccgcggtc gcggtgatcg tgacgacatc gccaccgtcg ccgatgtaga
     1801 cggtattgtc ggttagaaac tcgttgatct cataggcaac ccggtacgga ttgatcggcg
     1861 tggattcgga tttgaatgac ggcagcaatt tctcgtaagc tttaccttcc gcgctgcgca
     1921 attccttcat ccattcagcg cgtttcgcgt ttccattgga agcgcgcccg cttgccgcct
     1981 gcgctactgc gttcagaatc gcgccacaat cgcccaccag acccagcgag atgtcgcggt
     2041 tcttgccgac attgcggtaa tccaaatcga tttgcacgac cgtcgcatcg gcacgcaacc
     2101 ggcggccgta accgagacga aaatcgaacg ggctgccgac gatcaggatc acgtcggcct
     2161 tgtcgaacgc aagccgccgc gtccggttaa agccatgcgg atcgccggga gcgaaaatgc
     2221 cgcgcgccga accgttcata tagccgggga tattgagcgt gcggcaaaac tccaccgcca
     2281 cgtcatgccc gcggcaggtc caaacttgtg tgccgaatag cgcggcggga cgttcggctt
     2341 tgactagaat atcggcgagc cgctcgatat cggcgggatc gcccaccgat ttagtcgatg
     2401 cccggtaggc gcccggtttg ggaacccgcg cccgctccag cggcaccttg gcatcgagca
     2461 cgtcgcgggg gatttccaga tagctcggtc ccggggcgcc gttgaccgat tcgcggaacg
     2521 ccatcgagac catgtcggcg attcgttcgg tatgcggcac gctcgcagca aacttggtta
     2581 tcggtgacat gaaatcgacg tgaggcaaat cttgcagcga tcccatcttg tgctggctca
     2641 gcgccccttg cccgccaatc atgagaagcg ggctttcggc gcgaaaggca ttagcgatgc
     2701 cggtcatcgc gttggtggtg ccgggccccg cggtcgtaac aacgcaaccg attttaccgc
     2761 tctggcgcga ataaccatcg gcggcgtgtg ccgccacctg ttcatggcgc acgtcgataa
     2821 ttttgatccc ttcgtcgatg cagccgtcat aaatatcgat gatatgaccg ccgcacagcg
     2881 tccagatcac ctccacccct tcgttcttaa gcgctttggc gacaagatgc ccgccggaaa
     2941 tgaccggtcc agtcgcttct tttttggatt cagaattctc tgccatcgtt acctcgcctg
     3001 atctgctttg gaatacaatc tttccgctcc gaaactgtca ttgagcgtgc aaccgagtgg
     3061 agcaaagagc cagtccagtg cgcgcagcta tccgattctg cgcgcctgat cacgcagcac
     3121 ctctctatct cgcagactat tctgcccata gctttttgac agccggccgg actccagcac
     3181 actttcctcc acctgttcga tgtggcgcaa catttcggtg cgagcctttg ccttgtcatg
     3241 tttttcaatc gcggcgacga ttcttcgatg cgccgcgatc gacgatgctc gacggttgtg
     3301 ggtctgcaat gactcctcac ggctgcggct cagcagtttc atcaaccccg agactaattt
     3361 ctgcagcgcc tgatttcggg tggtacggct aatgaagaaa tgcagctcag catccgcctc
     3421 gacaccggtc tcgccgcggc ctaccgcctc gctctgcttg gcaacgattc tttttagttg
     3481 cgcaacgtca tcccgccggg cccgctccgc ggccaaagcg gcaatgtgcg gctcgatcaa
     3541 cttccgcatt tcgaacatgt ccaccagagc ctttttctca tcgatcaata gccgcgcgag
     3601 cggcccgatt agagattcta ccggcaagtc ggcaacgaaa ttgcccgtgc cggttcggct
     3661 gacgataagt ccttgggttt ccaacgctcg aagcgcctca cggaccgaag ttcggcttac
     3721 tttgaatgtt tcagccagtt cgcgctccga cggcagctga tcacgggccc tgaacctacc
     3781 ctcctcgatc agttgacgga tctggttgaa gacttcctcg tgaattcgaa ttcttttgat
     3841 tgcgcgaagc atcaaggacc gccggactgc tggtctgacc agttatcgaa tacccaagtg
     3901 cctgtcaagt tatataactt tgtgcgagag ccggaatcaa tagattgctt ttcggcagcg
     3961 ccggagcgtg tgtataggcg aactcttgac cttgccagtc tggactacta tgcataccca
     4021 gaacggaaga ttaaagtcag atgttagcag tgaaactaca atccgcgaga taagagccga
     4081 tgcctgatgc cgatctcact cgactcgatt cgatcctcac ccggttagcg ccgcaaggcc
     4141 gcgctgcgct cctgccggcg ctccacctcg cgcagcaact ccacggctgg attcccgaag
     4201 ccgtagcggc ggaagtgggc cgcattttgg gcgtgccgct cgccgatgtt cacggcgtta
     4261 tcgattttta tgagatgctt tcgcggcagc cgatcggccg aacggtggtg cgtgtatgca
     4321 acgcgccggt gtgcgggctc gccggagccg atgggctggc tgacgcgctg tgcggctatt
     4381 tgaaagttaa accagggcag gtcagcgccg acggagagtt tacgatcgag catgctccgt
     4441 gtttgggttt gtgcgatcac gctccggcac tgctgacagg cgattcagca atcggacgtg
     4501 ccgctcccga gcaggcagac acaatctgtg cgcggaaagt tgaaaagccg atcagctttg
     4561 tcggcggcga catccggatc ctgaccagca gttgcgggca gggccattcc actactttcg
     4621 ccgagtacag cagtggcggc ggttatgcgg cattgaaaaa agctttgacg atcacgcccc
     4681 aagacgtgat tgcggaggtg aaggccgccg ggttggtggg ccgcggtggc gcggcatttc
     4741 caaccggaat caaatgggaa ggagcggcca aggcggtcgg gcaacccaag tacatcgtgt
     4801 gcaatgcaga cgaatccgag ccaggaacat tcaaagaccg catcctcatg gaggaggacc
     4861 cgcatcgtac catcgaaggc atgatcatcg cggcccacgc cgtcggtgcg cgttacggct
     4921 acatctacat tcgtggagaa tatccctgcg cattcaaggc tatgaacgat gctgttgacg
     4981 aggcgcggcg gtctggcatc attggcgaaa atattctcgg ctcgggattg gacttcgata
     5041 ttgaaatgcg gctgggcgcc ggcgcctaca tttgtggcga agaaaccgcg ctgctggagt
     5101 ccatcgaagg caaacgcggt tttccgcgta tcaagccacc gtttccgacc acccacgggt
     5161 tgtttggtaa accgaccgtg atcaacaatg tggagacctt ctgcaacatt ccactgatta
     5221 tcgaaggcgg cgccgcagag taccgcaaaa taggcacaca acggtcgccc ggtcccaagc
     5281 tgttttgcgt atcggggcaa atacaggaac ccggactcta cgaggtgccg tttggcgtca
     5341 ctttgcgcca tttgctgttc gagctggcag gcgggttgaa gcaagggcgc aaacttcaag
     5401 ccgtgctgat gggcggcgct gcgggcgcgt tcgcctccga gaaagacttg gatgtgatct
     5461 tatcgtttga gaatctgagc gccgcgggtc tgccgctcgg ctcgggcgcg gtgatggtct
     5521 tcgacaatac tgcggatctg cgcgacgtgc tcaagcgact ggcgcgcttc tttgccgacg
     5581 aaagctgcgg caaatgctat ccctgccagc tcggcacgca gcgacaacat gaaattctcg
     5641 atcgtgccgc tgctggagtt gccgttgccg gcgaccggga acgcttatcc gatgtaggcg
     5701 gcacgatgat cgacgcttct ctgtgcggcc tgggacagac ggcgccgacg gcggtgttga
     5761 gcgcaatgaa gctatggccg gacttgttta aatgacccgc gccccacata ttggcggggt
     5821 gaagtactgg agtgatgtct ccgagccaat atttcattac tccaccactc caatgctcca
     5881 gaggggtttt tatggctgat acgacactaa ccatcgacgg cgagtccgtc acagttcctg
     5941 acggcacgac catcctcaag gcggcggaga aaaccggtcg gcaaattcct acaatctgct
     6001 accacgatca ttgcaccgcc aacgggctct gccgcatctg cgtggtcgaa gtcgacggcg
     6061 taaagccgct tgtcccggcc tgcctcagcc gggtgagcaa aggtatgaaa gtcgtcacct
     6121 cgagcgcgcg ggccgagcgc agccgccgga cgatcctgga gatgctcgcc tcggccgtgg
     6181 atctgtctga ggcgccggag attcaaaaat ttatcgattc gtatggcgcc gacacaacgc
     6241 gctttcccga cgccgagcgt cgcgagtatc cggtgctcga tgataacccg atgtacctcc
     6301 gcgattactc caaatgcatt ttgtgctggc gctgcgttca ggtgtgcgcc gaggatgcgc
     6361 agtacaccta cgcgctaaac ttcaaaggcc gcggctttga caccggcatc gcaactttct
     6421 ttgataaacc gatgcctgag acgacctgcg tattttg
//
LOCUS       DASUTG010000438         7103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_363422, whole genome shotgun sequence.
ACCESSION   DASUTG010000438 DASUTG010000000
VERSION     DASUTG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363422"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VF089_10030"
     CDS             <1..840
                     /locus_tag="VF089_10030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174343.1"
                     /translation="GGYSLLALTAPESRRGEASGLYSGVQGAASIIFPAVALWLLAAS
                     FGGFNVVFVTTALLSLLAAGVGAVMMRYSPRASRGAQVEQARPWWREVFHFVEPEIFL
                     PSVLLLWLNLALPSMTNFIVLYARELAVENFAIYFIICGITNLLARPGLGRLSDKIGR
                     GRSVAAGFILQVLALSLITLVSSLPGILIAGVLYMLGNAIGSATILALAVERADPRRR
                     GKAMATFSVAYPLSYGVGSLMIGSIVEIAGYVGMFLILATIEAGGLIFVHLNRRALKS
                     GAI"
     gene            1318..2124
                     /locus_tag="VF089_10035"
     CDS             1318..2124
                     /locus_tag="VF089_10035"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX6174344.1"
                     /translation="MVEAVTVDIPYTPETGEKLVNETFGPNNIRRRKTGADELKSMSV
                     HNGRLVVERLSLDEQGFVFVEHNTKVADFFDSEQLKSVYYPEVEELIKSASGASRVVI
                     FDHTLRSGDQTEREAKLIREPVLSAHNDYTEWSGPNRLREILPDEAESLLQHRFAIIQ
                     VWRAINQPIQSNPLAVADAKSVALDDFLIAERRYPHRVGQTYRLKYNPNHRWFYFPEM
                     RRDEALVFKVYDSETDGRARFTPHTSFKDPASPPNAPARQSIEVRAFAFF"
     gene            2291..3322
                     /locus_tag="VF089_10040"
     CDS             2291..3322
                     /locus_tag="VF089_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174345.1"
                     /translation="MKKIICFLSVFALMGGTAHASSHEFFKGKTIRLVIGNSAGGAMD
                     DWGRFVAQHLGRHIPGNPEVLVQNMPGAGAIVAANYIYNIAKPDGLTLGLINPANYTD
                     QLLGANEVKFDWPKFSWIGSPERIDQVLFIRTDVPYKTLDEMRNAKDPPRCAALARAG
                     LGYLLPRLFEEALGIKLNMVLGYGGGGEMNLAIEKGEVHCRAGTVSAYVGREPTRTWI
                     KNGVVRAITQSGTKRYPKLPDVPTLYELMDSHKTPEATRRVARVLLSSGEHGRPFIAP
                     PALPADRVKILRDAFNKAMTDPALLADAQKRKWDLDPLTGEELEATAKEIMVQPPEVI
                     DRMKKLMEN"
     gene            complement(3363..3653)
                     /locus_tag="VF089_10045"
     CDS             complement(3363..3653)
                     /locus_tag="VF089_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174346.1"
                     /translation="MHRLKTDRAFGLKIAAKYLRLEDKELLQRTYESSIDEKKLPAKQ
                     YPSVAGLKTILDQLADTNPKAKTAKPEDFIEARFVEEFDKSGYIDSLYGRKK"
     gene            complement(3658..4371)
                     /locus_tag="VF089_10050"
     CDS             complement(3658..4371)
                     /locus_tag="VF089_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174347.1"
                     /translation="MKNHRGLMWVVVVVSKMHWSSAVSAQPSKVAIGYSGISADQLVI
                     WVAKDAGIFAKNNIDAQTVYFSGGTTSVTAMISGDTPLIQASGPGIVSAGLAGADPIY
                     VVGGITTLDYWLMTQPEIKTPEQLKGGTIAVARFGGAADFVARYALVRLGLTPGKDVT
                     IVQTGSTPERLAAMESRRVSGSTLVPPAMFTAQKKGFHLLADVASLGLAYQHQGGATI
                     KKIPSRTSGRRPQLRQIVY"
     gene            complement(4683..6302)
                     /locus_tag="VF089_10055"
     CDS             complement(4683..6302)
                     /locus_tag="VF089_10055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6174348.1"
                     /translation="MDKVLDQILGLTDKGTVEQRCASLLVLGALKLQSSEIIKTVGSM
                     LDHANPVLKDYAVRYFEQVQAKAATPLFVKLLDDPDRDIQERAVRLLSQAGAAAVDPL
                     VRNSAGGSRSWQINAARILCAVQGSSARQALLEMLRAGSDDFNKSVCDLMTPAIRAMD
                     VKEQALFYDELEEYGKKLDEKQQRPTLVSVIRLLGQLGRADARRWLFKFIGPEHLPVL
                     RSHALVALLRCLREQDLRKDEYAKLFPLLEEVEMTEVTRLALELLDKHELPDDSRSLL
                     ARLLESRHPEVQKFALRKMAGFGTPGTVRTLIEQLGDTDYRRRDVAASSLRKMTEART
                     ALIKELLACEDPSKAWSIAELLTSFEGKWRQDTLEALWNRLQAAISADDRIQTAFLHI
                     LKKADVDQTYDQLARQGARLIKAKRYKEGLAFLNPLKDFPGFKPEQKFQIALAQLKLH
                     SHSVASHRQHPVLDLFTDLYRNSAFPLYEALKKEKSLAPEEYFALGFSLVERSGQERN
                     LGKDLLDHVAAKSPRTKIGKSAKNKLKLLNW"
     gene            complement(6471..7037)
                     /gene="pal"
                     /locus_tag="VF089_10060"
     CDS             complement(6471..7037)
                     /gene="pal"
                     /locus_tag="VF089_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX6174349.1"
                     /translation="MSVRSWSLLAMIVLLGCGNPVNPPEPKWTTENPNASSEKSATAA
                     AQKPVADTKTEKSGGGSLDDLQKGGTAASGPLKDVFFAFDRSDLSTDARSILKANSDW
                     LKKNPAAQVQIEGHCDERGTVEYNLALGSKRAQASKDYLETLGIPANRLSMISYGEEI
                     PVCRENNDACWEKNRRARFIVTSGRPAS"
BASE COUNT         1512 a         1932 c         1961 g         1698 t
ORIGIN      
        1 ggcggctatt cactcttggc gcttaccgct cccgagtcca ggcgcggcga ggcatccggt
       61 ctttatagcg gcgtacaggg cgccgcatcg ataatatttc ccgccgtcgc gctctggctg
      121 ctggcggcat ccttcggcgg attcaatgtg gtctttgtca cgacagcact gctttcactg
      181 ttggcggcgg gcgttggagc agtgatgatg cgctattccc cgcgggcaag ccgtggggcg
      241 caagtcgaac aagcacggcc gtggtggcgc gaagttttcc acttcgtcga accggaaatt
      301 tttctgccat cggttttgct gctgtggcta aacctcgcat tgccgagcat gactaacttc
      361 atcgtcctat acgcgcgaga actggccgta gaaaattttg ccatctactt catcatctgc
      421 ggcataacca atctgcttgc acgtccaggc ttaggccggc tttccgacaa aatcggccgc
      481 ggccggtctg ttgcagcagg atttatcctg caagtgctcg cgttatcgtt gatcactctg
      541 gtctcgagtt tacccgggat actgatcgct ggggtgttat atatgctcgg caacgcgatt
      601 ggcagcgcaa cgattttagc gctcgccgtt gaacgcgcag acccgcgccg ccgcggcaaa
      661 gcgatggcga cattttcggt ggcctatcca ctgagttacg gagtcggttc gctgatgatc
      721 ggcagcatcg tcgaaatcgc cggttatgtt gggatgttct tgattttggc tacgatcgaa
      781 gccggcggtt tgatttttgt ccatctgaac cggcgcgccc tgaagtccgg cgcaatctag
      841 gatactgcga cgcaaagctg cgcttgcgtg ttctagtccc gttgatcgac ggttctgttg
      901 attgatcaac gcgcgcctcc cgccttcgac attcgttcct taatggggaa tagcaattga
      961 caccgaatgg aaagcggaga tataggctgc ttacgggctt gcatttgagc gtcggcaaag
     1021 gggtcatata cggggaaaag ctcctgaagg aaatggggcg gaaatagtcg gacgaggagc
     1081 aaaaccgtga tcgtgtttgt gacgggagaa aaaccgtgtt cttgtccgga gaagtgacgt
     1141 gagcgtgatc ctgtgatcct cctccagata atttatacga tcaccaaggt tttcaggtca
     1201 cactatcgct ttatcctctc gggtgataac cgaacacgag aggcccagat cggctagggt
     1261 gtatttcgct accagttccc gtgtgataac ggtgacaata atcaaggagg gaattttatg
     1321 gttgaagccg tgactgttga cattccctat acgcccgaaa ccggcgagaa gctggtcaac
     1381 gaaaccttcg gcccgaacaa tatccgtcgg cgtaaaaccg gcgcagacga gttaaaatcg
     1441 atgagcgtcc ataacggccg gttggtcgtt gagaggctat cgctggatga gcaaggtttt
     1501 gttttcgtcg aacacaatac caaggttgcg gatttcttcg actctgagca gttgaagtcg
     1561 gtttactacc ctgaggtcga agagctaatc aaaagcgcct cgggcgcctc gcgcgtggtg
     1621 atcttcgatc atactttgcg ctcgggcgat cagaccgaac gcgaagcaaa gctcatccgc
     1681 gagccggtgc tctcggcgca taacgactac acggagtggt ccggcccgaa tcggctgcgc
     1741 gagattctcc ccgacgaagc cgagtccttg cttcagcatc ggttcgcgat cattcaggtg
     1801 tggcgcgcca tcaaccagcc tatccagtca aatccgctgg ccgttgccga tgccaagagt
     1861 gtcgcgcttg acgattttct tatcgccgaa cgccgttatc cgcatcgagt agggcagact
     1921 taccggttga agtacaatcc aaatcatcgg tggttctact tccctgagat gaggcgagac
     1981 gaagccctcg tttttaaggt ttacgattcc gaaaccgatg gccgggcacg gttcacaccg
     2041 cacacgtcgt tcaaagatcc cgcctcgccg ccaaacgctc cggcgcgcca gagcattgag
     2101 gtgcgcgcgt ttgcgttttt ctagcggtga gagccctaag ttacgaaagt tatagctgct
     2161 cacgctcgtg ttcgtgctaa cgtgctcgtg atcgggtcga tcgtttggat gtaggtttga
     2221 caattacgtc gtcacggttt gttcggcttt acgctttcag ctctcatgct tgattcagcc
     2281 ggaggccctt atgaaaaaaa ttatctgttt cttatccgtc tttgctctaa tgggcggcac
     2341 cgctcatgcg agctcccacg aatttttcaa aggcaagacc atcaggctgg tcattggcaa
     2401 ctcggctggc ggcgccatgg atgattgggg ccggtttgtg gcgcagcatt tgggccggca
     2461 cattcctgga aaccctgaag tcctcgtgca aaacatgccg ggcgcgggag cgatcgtggc
     2521 ggccaactat atttacaaca ttgccaaacc cgacgggctg acacttgggc tgatcaatcc
     2581 cgccaactac acggatcagc ttctcggtgc caacgaagtg aagttcgatt ggcccaaatt
     2641 ttcctggatc ggctctccgg aacgcataga ccaggttctc tttatccgca cggatgttcc
     2701 gtataaaacc ctggatgaga tgcgcaatgc taaagatccg ccgagatgcg cggcgttggc
     2761 gcgcgccgga ttgggttacc tcttaccccg gctattcgag gaggcgctcg gcatcaagct
     2821 caacatggtg ctcggttacg gcggcggcgg cgagatgaat ctggccattg aaaaagggga
     2881 agtgcactgc cgtgccggaa cggtgtcggc ttacgtcggc cgcgaaccaa cccggacatg
     2941 gattaagaac ggagttgttc gcgctatcac ccaaagcggc acaaagcgct atccgaaatt
     3001 accggatgtt ccgacgctct acgaattgat ggacagccat aaaactccgg aggcaacaag
     3061 gcgggttgcg cgtgtgttgc tttcatccgg cgagcacggc cgtcccttta tcgcgccacc
     3121 ggcgctgccg gccgatcggg tcaagatttt acgcgatgct tttaacaaag ccatgactga
     3181 cccggcgctc ctagccgacg cgcagaaaag aaaatgggat ctagatcctc tgaccggtga
     3241 agagctggaa gcaaccgcca aagaaatcat ggtgcagcca ccggaagtca tcgaccggat
     3301 gaagaagctg atggagaatt gacacagcga cagagcgctc ctcgatcact gattattgat
     3361 cgctacttct tgcggccgta aagactgtcg atatatccgc tcttatcgaa ttcttcgaca
     3421 aaacgggctt cgataaagtc ttccggcttg gcggtcttag ccttaggatt ggtatcggcg
     3481 agctgatcta aaatggtctt tagcccggca accgatgggt actgcttggc cggcagcttc
     3541 ttctcgtcaa tcgagctctc ataggttctt tgcagcagct ctttgtcttc gagccgcaga
     3601 tatttggccg caatcttcaa tccaaatgca cgatcggttt tgagtcggtg cacggactca
     3661 atatacgatt tgacgaagtt gcggacgacg tccggatgtt cgcgaaggta tctttttaat
     3721 cgtagcgccg ccttgatgct ggtatgccag gcccaaagac gcgacatcgg ccagcagatg
     3781 aaagcccttc ttttgcgcgg taaacattgc cggcggcacc agggtgctac cgctgacccg
     3841 gcgcgactcc atcgccgcca aacgttccgg agtgctgccg gtttgcacga tcgttacgtc
     3901 tttgccggga gtaagcccaa gccggacaag cgcatagcgc gccacgaaat cggccgcgcc
     3961 gccgaagcgc gccactgcga tggtgccgcc tttgagctgt tccggcgttt tgatttccgg
     4021 ttgagtcatt agccagtagt ccaaggtggt gatgccgcca acgacataga ttggatcggc
     4081 gccggcgaga ccggcgctga cgattcccgg tccagacgct tgaatgagcg gagtgtcgcc
     4141 ggaaatcatc gcggtaaccg aggttgtgcc accggagaaa tagacggttt gcgcatcgat
     4201 attgtttttg gcaaagatgc cagcgtcttt cgctacccag ataaccagct ggtccgcgct
     4261 gatgccgctg taaccgatgg cgactttgct cggctgcgcc gagaccgctg agctccagtg
     4321 cattttggac acgacgacta ccacccacat cagccctcga tgatttttca tgttgtttcc
     4381 tcgcgcttta cagattgcag cccggattat tcatgctcag taaaaactac gacactgtct
     4441 ccatcgaagg gtgagcctgg aggagtaaag acacctagac gaacagttca atgacgtctt
     4501 tggtgcttga tgcccgctcg cattttttca gatggtcgct ccagaaactg tgcttacagg
     4561 ccgctccaag gcaccggccc atttgtcttt gctccggagg gctcaccctt cgatcaccgt
     4621 cacgataatc tggtttacgt gatgctgcgc cgttggcata aagatcggcg tcaggcaagc
     4681 cctcaccaat tgaggagctt caatttattc tttgcgctct taccgatttt ggtgcgcggc
     4741 gatttggcgg cgacatggtc taacagatcc tttccaaggt tgcgctcttg gcccgatctt
     4801 tccaccagac tgaagcccag cgcgaaatat tcctccggcg cgagactctt ttctttcttt
     4861 aacgcttcgt acagcgggaa cgccgagtta cggtacaaat cggtgaacag atcgagtacc
     4921 gggtgctgtc ggtgactggc cactgaatga gaatgtaatt tgagctgcgc cagggcgatt
     4981 tgaaattttt gctccggctt gaacccggga aagtctttca gcgggtttag aaaggctaag
     5041 ccttccttgt acctttttgc cttgatcagc cgcgccccct gccgggcaag ctgatcgtaa
     5101 gtttgatcaa cgtcggcttt cttcaggatg tgcaagaaag cggtttgaat tcgatcgtcc
     5161 gcgctaatgg ctgcttgcag tctgttccag agcgcctcca gggtgtcctg gcgccacttg
     5221 ccttcgaacg aagttaacag ctcggcgatg ctccatgctt tgctcgggtc ctcacatgct
     5281 agaagctcct tgatcagagc ggtgcgagcc tctgtcatct tgcgcaagga acttgctgcc
     5341 acatcgcgac gccggtagtc ggtgtcaccc agctgttcaa tcaaggtccg caccgttccg
     5401 ggtgtgccga agccggccat ttttcgcaaa gcaaattttt gcacttctgg atggcggctt
     5461 tccaacaagc gcgccaacag cgatctggaa tcgtccggca gctcgtgttt gtcgagcagc
     5521 tccagggcca aacgggttac ttccgtcatc tcgacttctt cgagtagcgg aaacagtttg
     5581 gcatattcgt ctttgcgcag gtcctgctcg cgcagacaac gcagcaaagc tacgagcgcg
     5641 tgagagcgga gcaccggaag gtgttcggga ccgatgaact tgaacagcca gcgacgcgca
     5701 tcggcgcggc cgagttggcc caataagcga atgaccgata ccaaggtggg tcgctgctgt
     5761 ttctcgtcaa gcttcttgcc atactcttcg agctcatcgt agaagagcgc ctgctcttta
     5821 acatccatcg cgcggatcgc cggcgtcatc aaatcgcaaa ctgatttgtt gaagtcatcg
     5881 cttccggccc ggagcatttc caacaaggct tgacgcgcgc tcgatccttg aaccgcgcag
     5941 agaatgcgcg cggcattgat ctgccaggat cgtgatccgc ccgcagaatt tctaaccaac
     6001 ggatcgaccg cggcggcgcc tgcctgactc agcaggcgca cggcccgctc ctggatatca
     6061 cgatccggat cgtcgagaag ttttacaaac agcggggttg cagccttagc ttggacctgc
     6121 tcgaagtaac gcacggcata atctttgaga acgggattgg catgatcgag catcgagccc
     6181 acggtcttga tgatttccga gctctgtaac ttaagagctc ccagcaccag caatgaagca
     6241 caacgttgct ctacagtacc cttgtcggtc aacccgagta tttggtccag tactttgtcc
     6301 atgtgagggt tgaacgtcat ccatggccaa aagtcaagaa atcggtttga ttgcgcgaga
     6361 ccataaaaaa ggcccaatct cggatgagat tgggcctccg gaagtcaagt tgaagcctat
     6421 ttgatcggat gaattcttga cagccagcct ttaagcgaag ctatcggcgg ttacgatgcg
     6481 gggcgcccgg atgtcacaat gaaccgagca cggcggttct tttcccaaca cgcatcattg
     6541 ttttctctgc aaaccgggat ttcttcgccg taactaatca tcgacagcct gtttgccgga
     6601 atgccgaggg tctcgagata gtccttgctt gcctgcgcgc gtttggaacc caaagctagg
     6661 ttatactcga ccgttccgcg ctcatcgcaa tggccctcga tctgaacttg ggccgccgga
     6721 tttttcttga gccaatccga atttgccttc aggatcgaac gggcatctgt cgataaatcg
     6781 gatcggtcga aagcgaagaa aacgtctttc agcgggcctg aagcggctgt gcctcctttt
     6841 tgtaaatcat ctaggcttcc cccaccggat ttttcggtct tggtgtcagc gacgggtttc
     6901 tgagccgccg cggtcgctga tttttcagag gacgcgtttg gattttcggt cgtccacttt
     6961 ggctctggag gattgaccgg atttccgcaa cctagcaaaa caatcattgc taataagctc
     7021 caactgcgaa cagacattta gactctcctt tcgaactggt gattacttgg gttgagaggc
     7081 cggacaagaa gttgaaacgg ctc
//
LOCUS       DASUTG010000439         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_660736, whole genome shotgun sequence.
ACCESSION   DASUTG010000439 DASUTG010000000
VERSION     DASUTG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660736"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF089_10065"
     CDS             complement(<1..466)
                     /locus_tag="VF089_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007894067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174350.1"
                     /translation="MSEKNFEPFTFNRAVERPALRNGQMPPSNGSAHLSSANGALHGD
                     NDSSLNGRQLPKYRENFPIVVHCHLRWDFVWQRPQQIFSRMAAHHPITFIEEPMWQGS
                     ERRLHISEPYPNVVRIIPVLRAEEKNDEMDNQYAAVLALLRSALSENPLLSAR"
     gene            825..971
                     /locus_tag="VF089_10070"
     CDS             825..971
                     /locus_tag="VF089_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174351.1"
                     /translation="MKNSTVTLLLDQLRRESQKQKEDGDLKDGEYQEITELIGQIERA
                     FTNG"
     gene            complement(983..2068)
                     /locus_tag="VF089_10075"
     CDS             complement(983..2068)
                     /locus_tag="VF089_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174352.1"
                     /translation="MKRLLQAINVLPEAMLLIHGNGIILGSNSAARKLFGRDLEEGIS
                     LNSLVSDSSEKVLRSLRLWSSSRQFLPASLQINRGGRPVVIRCDGAAVEPATPHQSSL
                     LLLRCVLRGESETTKFFIRLSAKIDDLWKQNAEQKARDHDRLSSLATAAAVFAHEVAN
                     PLNGIGMSVQLLEKEFLQRHDVSPVLRESIVEANKEIARLSSLLRDFRSFARTQFVEF
                     QPTDLSTLVQEVLAPEMVLFSNSGVRVRSYLSGLPPIRLDPDKMKQVILNLCKNAVEA
                     MPEGGCLTLKGYLSSADTIVLEVSDTGIGIPEGMDIFEIFRTTKPDGTGLGLPLVNQI
                     MSAHRGTVDYVSEPGKGTTFRLSLPVR"
     gene            complement(2079..2591)
                     /locus_tag="VF089_10080"
     CDS             complement(2079..2591)
                     /locus_tag="VF089_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="HEX6174353.1"
                     /translation="MRLDEMDIPLERDVFLRSLIRELSGVLEDVVGVKEASGFVSVVG
                     RSIGEWMNGEYRKALSVSNLSRDQVAQVCVDLKRRIQGKFYIVEEDEDKIIFGNTACP
                     FGDKVIGRESMCMMTSNVFGHIAAQNVGYAKVELQETIARGDQGCRVVIYLQPASASG
                     EGREYFRTDS"
     gene            complement(2604..2969)
                     /locus_tag="VF089_10085"
     CDS             complement(2604..2969)
                     /locus_tag="VF089_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174354.1"
                     /translation="MPPTILLVEDNPLARRNMMLFFKTHGYDVLEAQRGEEALKLIRD
                     VDNFDVVITDLRMPGMVDGLEVLRVQNEVSPGTKTILVTAYGSNQIKYQAAELGAVYL
                     DKPVQLDDLLVHVRQLTSP"
     gene            4017..4277
                     /locus_tag="VF089_10090"
     CDS             4017..4277
                     /locus_tag="VF089_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174355.1"
                     /translation="MSELLETVRQYTREYIRLRKETNQLLRQGKIDKQEASHRNGNAK
                     QFLMFARYQLETAKKAGDLKRPASPSHNWKRESEDNWHSKER"
     gene            complement(4293..>6056)
                     /locus_tag="VF089_10095"
     CDS             complement(4293..>6056)
                     /locus_tag="VF089_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174356.1"
                     /translation="FAVCEAIKADPATAHIMVLQISAMYLSPEDRATGLERGADTYLT
                     EPVEPAELLGATKALLRLYERDQEKRFLLDQLREANRQNEAQLAELKSIYETAPVGLA
                     VLDTELRYLRVNDLLAVKNGIPADQHIGRTVREIIPSIAGAVETACRELIATGKPRLN
                     VEMTGETAAQVGLKRTWLNSYFPLKDLNGDCIGINVVVQDLTDQKRLQERLRDADKRK
                     DEFLAVLGHEWRNPLGVISNAFELLPASETPESQEIRAIIKRQITRMTHLTNDLLDVS
                     RISAGQIGIDKQSCDFAAIVREAAEDYRGILNSVGIRLELQLPRLPIWVLGDCRRLAQ
                     SVGNLLHNASKFTPPGGTVTVKLELVAHQPTAVLKVRDTGIGMDQSTLTRLFEPFSQA
                     FRTFDRSRGGLGLGLTLVKGFIDMHGGEVSASSKGPGNGSEFTVQLPVQGDPSGTSDA
                     HPESIACRSNDPISCRILIIDDNQVGARAMQLFLQDAGHRVEIAHSGVEGLVIARNLI
                     PDVVLCDIALPWMDGYTISRTIRQDAELKDVYLIAVSGYAQDEDQELARHAGFDEYCV
                     KPVELKSLDNLIKNRLSARAR"
BASE COUNT         1469 a         1635 c         1554 g         1398 t
ORIGIN      
        1 accgcgcact gagcagtgga ttttcgctga gcgcgctgcg cagcaacgct aatacggctg
       61 cgtattgatt atccatctcg tcattttttt cctcggcgcg cagaacaggt attatgcgca
      121 cgacattagg atacggttcg ctgatatgga gccggcgctc gctaccctgc cacatcggct
      181 cttcgataaa ggtaatcgga tgatgcgcag ccatgcggga gaatatctgc tgcgggcgct
      241 gccaaacgaa atcccaacgt aagtgacaat gcacgacgat cgggaaattc tcgcggtatt
      301 taggcaactg tcgaccgttt agactcgaat cattgtcccc gtgtagcgct ccattcgcac
      361 tcgagagatg ggcggaccca ttgctcggcg gcatctggcc gttgcgcaga gcgggccgtt
      421 caacggctct gttgaaagta aaaggttcga agttcttttc agacatagtt ctcctattcc
      481 tgtgcgttgg gtttcgcgcg ctcatcagac agcggtgcgt gcgcatccaa ccaggaccga
      541 tcgcttcgcg gcccgcgtga ctatgaaggc aaacgtgtat gccgctcagc gacgagagct
      601 acctgtgtga caggttatcg caggagggcg tacagcctta cagcaaaggt cgttccaaaa
      661 ttaattccag acaatccgat tcaggcgtgt cgaatgagtg acatacgtcg attcaatgtc
      721 ctacaactgg aatcgcgtgt gacttcgggt ggatggttgg agagctcctc aaaaaaacct
      781 gctctccatg gtagaaacat ctgtgaaaga gaggatttta gtaaatgaaa aattctacgg
      841 tcaccctgct gctggatcag ctccggcgag aatcgcagaa acagaaggaa gacggagatc
      901 tcaaggacgg cgagtatcag gaaataactg aattaatcgg ccagattgaa cgagctttta
      961 ccaacggctg agttaggcgg aattatctta cgggtaacga cagcctgaat gttgttcctt
     1021 ttcccggctc gctgacgtaa tcaaccgtgc cgcggtgagc tgacattatt tggttgacca
     1081 acggcagacc taaaccggtg ccgtcgggct tggtggttcg gaagatttca aagatgtcca
     1141 tgccctcggg aatgccgatc ccggtgtcgc ttacttctag aacgatcgtg tccgcgctgg
     1201 acagataacc ttttagagta aggcatcccc cttccggcat ggcctctact gcgttcttac
     1261 ataggttcag aataacttgt ttcatcttgt cggggtcaag cctaatcggc ggcaaaccgc
     1321 taaggtaaga tctaacccgc actcccgagt tactgaacaa taccatctcg ggcgctaaca
     1381 cttcctgcac caatgtggaa agatcggttg gctgaaactc aacgaattgt gttcgcgcga
     1441 agctccgaaa atcccgcagc aacgatgata acctagcgat ctctttattg gcctcaacga
     1501 tgctctcgcg cagcaccgga gagacatcgt gtctttgcag aaattccttc tccagtagct
     1561 gcacggacat gccgatccca tttagcggat tagccacttc atgtgcaaaa accgcggcgg
     1621 cagtggcgag cgagctcaaa cggtcgtggt cccttgcctt ttgctcggca ttttgtttcc
     1681 aaagatcgtc gatcttagcg cttagccgga tgaaaaattt cgtcgtctcc gattcacctc
     1741 tgagaacgca tcgcaacagc agcaacgagg attgatgcgg agtcgcaggc tctacggctg
     1801 cgccgtcgca gcgaatcacc actggccggc cgccgcggtt tatctgcagc gatgcaggca
     1861 ggaattggcg gctactcgac cacagccgta aagaacgaag taccttctcg gaagaatcgc
     1921 tgaccagcga gtttagcgaa ataccttctt caagatcacg accgaacaat ttgcgcgcgg
     1981 ctgagtttga gccgaggatg atgccgttcc catgaattag aagcatcgcc tccggcagta
     2041 cattgatggc ttgcagtaat cgtttcatct agcatggatc agctgtcggt tcggaaatac
     2101 tccctgccct cgccggatgc cgaagcgggc tgaagataga tcacgacacg gcagccttga
     2161 tcgccgcgtg caatggtctc ctgaagctcg accttggcat aaccgacgtt ctgggcggcg
     2221 atgtggccaa aaacgttgga agtcatcatg cacatggatt cccgtccaat aactttatca
     2281 ccgaacgggc acgccgtgtt tccgaaaatg atcttatcct catcctcttc tacaatgtaa
     2341 aactttcctt ggatacgcct tttaagatca acacacactt gcgccacttg gtcgcggctg
     2401 agattcgaaa cgctcagggc tttgcgatac tcgccattca tccattcgcc aatcgatctt
     2461 ccgactacgc tgacaaatcc cgaggcttct tttacaccga cgacatcttc gagaacgccg
     2521 gacagctccc gaataagaga gcgcaggaag acgtcgcgtt caagaggaat atccatttca
     2581 tcgagtctca tgttatccct ccatcaggga gacgtcagct gtcggacatg aaccagcagg
     2641 tcatcgagct gaaccggctt gtcaagatac accgcgccga gctcggcggc ttgatacttt
     2701 atctggttgg agccatatgc cgttaccaga attgtttttg tgccgggcga tacttcgttc
     2761 tgaacccgaa gaacctctag cccatcgacc attcccggca tccgcaaatc agtgatcacg
     2821 acatcgaagt tgtcaacgtc ccgaatcaat ttaagagctt cttctccgcg ttgcgcttcc
     2881 aaaacgtcat aaccatgggt cttgaaaaaa agcatcatgt tacggcgggc caaagggttg
     2941 tcttcgacga ggagaatggt cggtggcata acttcttcga ttatacaatt aaacccgccg
     3001 tagcaagtag ggcctctgca gccctcgtcg ccgggcaaca cggtcgacct acagacaggc
     3061 aaaccctctg tacgtttgtg aaactaacga caatgtattg agccgacgag tcgatcaggc
     3121 acccgcgaaa tacattgcgg aaacgactga taatcgccga gccgtttcaa atgcataata
     3181 ggggagcatg ttccagcgat gcgacgcgcg gaccactcac aactacaaag cggtccgcgc
     3241 gtctccctcg ttcgaactgc ttagcgatgc cgttcgggtc tggcttgttc gcggctccaa
     3301 ctgttgaacg gcggactcag cctcccgcct gttaccaaac aaaccaatga tattcgccat
     3361 agttttctcc tacttgttta aatagcggac aataatcact gaatgacgag aagcgttgct
     3421 ttacttacga cgaggaccgc tctcgttctc agagttctcg attcaatgaa aatggatgcg
     3481 gtagtaactc aggaatgcga gccgccactc taatcagcaa tcagagtgcc agtatgatta
     3541 ggcgagcccg agcagcgaaa acaccctaag ccgcgcgtga cattatctcg ggaggagacg
     3601 aagcgacctt cgattgttcc ggcggtgcta cgactgcgat cagccggatc tcacctataa
     3661 gaatgtggaa gcttaatttt ttttggctgt tgttgatgca gttgactagc tctcgcgttg
     3721 atcccacttc gatgattgtt aaagagccgg cctgagctgg aattgccagg tcggatcacg
     3781 caggtgctaa ttactgaatc ctccacgaat atcacctcta attactactt gacttgcgca
     3841 accgattaga agtatcaatc taacactcag ccagttacga atcacacgaa tctttaaagc
     3901 gacgaaccgg atgaacggaa tgcaagcttt tttactgcat ccttcagttc accgtggaat
     3961 aggatgtcgg ttagaccctg tgaccgatgg tctcagagca catgaaagga tgggaaatga
     4021 gcgagttgct agaaaccgtc aggcaataca cgagagaata catcaggctt agaaaggaaa
     4081 cgaaccagct tcttagacaa ggaaaaatcg acaagcaaga ggccagtcac cgaaatggca
     4141 acgccaagca gtttttgatg ttcgcgcgct atcaactcga aaccgcgaaa aaagcgggcg
     4201 acctaaaaag acccgcaagc ccgtcgcata actggaagcg agagtcagaa gacaactggc
     4261 attcaaagga gcgataactg cacgacgtgc gcctagcgcg ctcgagcact taggcgattc
     4321 tttatcaggt tgtcgaggga tttaagctca accggcttga cgcaatattc gtcaaaaccg
     4381 gcgtgtctgg ccaactcctg gtcctcatcc tgtgcatacc cggagaccgc aatcaaataa
     4441 acatctttga gctcggcatc ctgccggatc gtccgtgaaa tcgtgtagcc atccatccag
     4501 ggtagggcga tgtcacagag cacgacatcg ggtatcaggt ttctcgcgat cactaaccct
     4561 tcgactccgc tatgggctat ctcaacacgg tggccggcat cttgaagaaa caattgcatg
     4621 gctcgggcgc caacctggtt gtcgtcgatg atgaggatac ggcatgagat cggatcgttc
     4681 gatcgacaag cgatcgattc agggtgggcg tcgctcgtgc ccgaaggatc tccttggacc
     4741 ggcaactgaa cggtaaactc tgaaccgttt ccgggtccct tactgctcgc ggacacctcg
     4801 ccaccgtgca tatcaatgaa tcccttcact aaagtcagac ccaagccgag accgccgcgg
     4861 ctacgatcga aagttcggaa cgcctggctg aatggctcga acaaacgggt caacgtgctc
     4921 tgatccatgc cgatcccggt gtcacgaacc ttgagaacag ctgttggttg atgcgcaacg
     4981 agctcgagct tcaccgtcac cgtaccgccc ggtggcgtga acttactggc attgtgcaat
     5041 aaattgccga ctgattgggc caaacgcctg caatcgccta gcacccagat aggaagcctc
     5101 ggcaactgca gctccaggcg gattccgacg gaatttagga tccctcgata atcctccgct
     5161 gcttcgcgca caattgcggc gaaatcacag gactgtttat cgattccaat ctggccggcg
     5221 gagatccgcg aaacatcgag caggtcgttg gtgagatgtg tcatacgagt gatctgccgc
     5281 ttgatgatag ccctgatttc ttgcgattcc ggtgtctctg aagcgggcag cagctcgaag
     5341 gcgttgctga tgacgcccag ggggttgcgc cactcatgcc cgagcacggc taagaactca
     5401 tctttcctct tgtcagcgtc gcgtaatctc tcttgcagtc gtttttggtc ggtgagatcc
     5461 tgaaccacca cattgatgcc gatacagtcg ccattcagat cttttaacgg gaaatagctg
     5521 tttagccatg ttctctttaa acccacctgc gcggcggttt cgcctgtcat ctcaacattg
     5581 agacgaggct tccccgtagc gatcaattcc cggcacgccg tttccaccgc gccggcgatg
     5641 cttgggatga tttcgcggac ggttctaccg atgtgttggt cggccggtat gccattcttt
     5701 actgcgagca aatcgtttac ccgaagataa cgcaactcag tatctagtac cgcgagaccc
     5761 acaggagctg tttcataaat cgacttgagt tcggcgagct gtgcctcatt ttgacggttg
     5821 gcctcacgaa gttgatcgag gagaaagcgt ttctcttgat cgcgttcgta gagacgcaac
     5881 aacgcctttg tcgcccctag aagttccgcg ggttcaaccg gctcggtcaa atacgtgtcg
     5941 gcgccgcgct ctagtccagt agcgcgatcc tcaggactca ggtacatagc cgaaatctga
     6001 agaaccataa tgtgcgcggt ggccgggtct gctttgatcg cttcgcagac tgcaaa
//
LOCUS       DASUTG010000440         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1104406, whole genome shotgun sequence.
ACCESSION   DASUTG010000440 DASUTG010000000
VERSION     DASUTG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104406"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(663..2348)
                     /locus_tag="VF089_10100"
     CDS             complement(663..2348)
                     /locus_tag="VF089_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174357.1"
                     /translation="MDYDLLIRGGRVVDGSGLPSYIADVGIKNGKVAEMGRLKGSAAR
                     TIEADGLIVAPGFIDHHTHMDGQILWDPYGTCEPQHGVTSIIMGNCGLTLAPVKAGGE
                     DALIKSFVRVEAIPRIALEKGVPWGWESYGDYLDRLEGNVGINVGGIVGHIPVRQYVM
                     GEESVERAATADEIEKMRQLVSESIFGGALGLSTNRNERHMREDGLPVPSRLATEEEL
                     LALCDVTRELNSGVIQTNHGRHCVEQIPEYDRIVRRTYRPLVWQSVQYKESEPELWKN
                     MLDGIANTFRDGYQAYGLTHTVPLMRHFSMKDAQVFDEFPVWKNLMFLPEQARKQAFA
                     DSETRKKMRDDMAEPRPVSFHRNWGRVLVEKVAKTENQKYVGQSVAEVAALRNQDALD
                     AFLDLSLEENLETTFETTNRGFNPEVMSTIMKSPYVMIGTSDAGAHVQFGADFGYCTT
                     LLGMWVRDRQLISLEQAIHKLTFHVASIYGIEGRGLLRPGFAADVTIFDPKTIKASAP
                     EWAEDYPAATKRLIQRSEGICQTIVNGKVIYEEGKLSGEMAGTVLRGTAHRAQ"
BASE COUNT          474 a          715 c          634 g          559 t
ORIGIN      
        1 atattgccta acgccacgct ctgcggcgcg cgcgttcaaa actctcctca ctagcgcgcc
       61 cgaagaagcg cgatgttatg cggaggagtt ctccttttcg acaatctctg aaagtccatt
      121 gaggttctcg gtcagctgcc aggccgctca caataggtgc ttacatcaga ctcgagaaga
      181 tctgatctct ccaaaatgat tttctttttc cagtctgaaa gcagcgcgtg aatgctgtgc
      241 gcaagaagtt tctctggctc ttcaacacct cgagcttggc tgcatctacg aggagagtgt
      301 gtttttgaag tagccggaga agcgcgccta gatttgcgtg ctcgtacttg agttcggggc
      361 gcgtggtctt tagatatcgc tttagtgctg tctcaagcct gcctgcagcc aacatcgcac
      421 gcccaagttg tgcacagaaa tcgtcatcag cgcataagag cgcaagaatt ccgtgccttc
      481 gacgggtcgc ccctcaaatt tccggattac cgacccgaaa caccgccgct ctgcgagatt
      541 catgtcgttg atcaagagtg gtcaatcagg tttcgttagc gttcatgcca atacacctgc
      601 agacgatcga ccccctcgca gcattgaact gtacaacgcg tcagtcccga gtgtggaagt
      661 ttttactgag cccgatgcgc cgtcccgcgc aaaactgttc ccgccatctc gccgcttaat
      721 tttccctcct cgtaaatgac cttgccattg acgatggtct ggcagatacc ttcggaacgc
      781 tggattagcc gcttcgttgc cgcaggataa tcctccgccc attccggcgc gctggctttg
      841 atcgtcttcg gatcgaagat cgtcacatcg gcggcaaagc cgggccgcag caatccgcgg
      901 ccttcgatgc cgtatatcga agctacgtgg aaggtgagct tatgaattgc ctgctccagc
      961 gaaatcaact ggcgatcgcg cacccacata cccaataacg tcgtgcagta gccgaagtcg
     1021 gcgccgaatt gaacatgggc gccggcatcc gaagtgccga tcatcacgta gggacttttc
     1081 atgatcgtgc tcatgacctc gggattgaag ccgcgattgg tagtctcaaa cgtggtctcc
     1141 aggttttcct cgagcgatag atcaagaaaa gcatcgagcg cgtcttgatt gcgcagcgcc
     1201 gcaacctcgg cgacgctttg gccgacgtat ttctgatttt cggttttggc aactttctcg
     1261 acgagtactc tcccccaatt gcgatgaaac gacactggcc gcggctcggc catgtcgtcg
     1321 cgcatcttct ttcgggtctc ggagtcggcg aaggcttgtt tgcgcgcttg ttccggcaag
     1381 aacattaaat tcttccacac cgggaattca tcgaagacct gcgcatcttt catggagaaa
     1441 tggcgcatca acggcaccgt atgggtgaga ccgtaggctt ggtagccgtc gcgaaacgta
     1501 ttcgcgatgc cgtcgagcat atttttccag agctccggct cgctttcctt gtactgcacg
     1561 ctttgccaga cgagcggccg gtacgtacgc cgcacgattc tgtcgtattc cgggatctgc
     1621 tcgacgcagt gacgcccatg gttggtttga atcacgccgc tgtttaattc tcgtgtcaca
     1681 tcgcagagcg ccagcagctc ttcttcagtg gcgagccggc tgggaacagg gaggccatct
     1741 tcgcgcatgt gccgttcgtt gcgattggtc gataaaccca acgcgccgcc gaaaatcgac
     1801 tcgctcacga gctggcgcat cttttcgatt tcatcagcgg tggccgcgcg ttcgaccgac
     1861 tcctcgccca tgacatactg gcgcaccggg atgtgcccga cgatgccgcc gacgttgatg
     1921 ccgacgttgc cttcgagccg gtcgagatag tcgccgtagc tctcccagcc ccacggcacg
     1981 cccttctcca gcgcgatgcg cggaatcgct tcgacacgga caaagctctt tataagggcg
     2041 tcttcgcctc ccgccttcac cggcgccagg gtcaatccgc aattgcccat gatgatcgag
     2101 gtcacgccgt gctgcggctc gcaggtgccg taggggtccc agaggatctg cccatccatg
     2161 tgcgtgtggt gatcgatgaa gcccggcgcg acgattaaac cgtccgcctc gatggtgcgc
     2221 gcagcgctgc ccttgagccg ccccatttcg gcgaccttgc catttttgat tccgacgtcg
     2281 gctatataag aaggcaaacc ggagccgtcg acaacgcgac cgccgcggat cagcaaatca
     2341 tagtccattt ctccgtcccc caatctttga cgttattcta at
//
LOCUS       DASUTG010000441         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1402300, whole genome shotgun sequence.
ACCESSION   DASUTG010000441 DASUTG010000000
VERSION     DASUTG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402300"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VF089_10105"
     CDS             complement(<1..1585)
                     /locus_tag="VF089_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX6174358.1"
                     /translation="MNLNERFLRQPCLAVASIILIVLSGIVGSAKTKVSRAMGQSSPA
                     GETILSLSDFDPVGDGVTDDGPALQRALDALADAGGGTLLIPAGQYLIATPVERDFSS
                     INSAQVKIQGVPSDTMPASPHANGQFLAAGLDLVSEFIPATGDKDDAIRLSNLAEFTI
                     EHVGFTGRPTAFTDAFVTLYLSDIDKATIRHCEFYGISTFGLRPGFGGGNLVRAVRSE
                     LSIELTVFLGCTANSGAYAPVVENVEWKKFTISNSIFLDYGLRPFFGKTGLGAPLSWI
                     NIANAAAPTPDSQRREVVMRDIFLDEGGWVGISAFPRNWGPATAPIDLIYISGLKMNV
                     SNLGTAGHLFYDVKNLLIENSHYSWSHNTVAAIYVNRTENVILDRLACIDDADRIRTD
                     ENTGRITIINSEFGGLDSLAFTTTVLETEPEDDPVQYVRQQFILMLSREPDPAAHFYW
                     SDLLIKCGDNNDCLNQKRSELSDYLKNDPQRDFSIAGTVSNEEGAPVSGAIINLTGSQ
                     SSSAVTDSQGKFRFTGLPTS"
     gene            2064..2426
                     /locus_tag="VF089_10110"
     CDS             2064..2426
                     /locus_tag="VF089_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6174359.1"
                     /translation="MSTKLYVGNLAFQTTSEDLQQLFAQAGTVQSANVVEDRETGRSR
                     GFGFVEMATNAEAVAAIDQFNGKEMAGRALKVNEAKPRENRNASTGRNFGGGNRGGFR
                     GGNHGGNNGGGNRREPRW"
     gene            2551..>2874
                     /locus_tag="VF089_10115"
     CDS             2551..>2874
                     /locus_tag="VF089_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015464560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6174360.1"
                     /translation="MNFSQLGLAPAQVSACESLGYKTPTPIQRQAIPVILSGRDVIGC
                     AETGTGKTAAFLLPIIQRLMESARPGLRVLVLAPTRELALQIQENYTALNNVKTNRSV
                     LAIGGA"
BASE COUNT          709 a          744 c          762 g          659 t
ORIGIN      
        1 cgctggtggg caaacctgta aaccggaatt taccctgtga atctgtcact gcggaagatg
       61 attgtgagcc ggttaagtta atgatggcgc cgctaactgg agcgccctct tcatttgata
      121 ccgtgccggc tattgaaaaa tcccgttgcg gatcattttt taagtagtcg ctcagctcgg
      181 aacgcttttg attaagacag tcattgttat cgccacactt tatcagcaaa tctgaccagt
      241 agaagtgcgc cgcgggatct ggctcccgac ttaacatcaa aatgaattgc tggcgaacgt
      301 actgcacggg atcgtcttca ggctcggtct ccagcacagt cgttgtaaat gccagagaat
      361 cgagtccgcc aaattccgaa ttgatgattg tgattcgccc ggtattctca tcagtgcgta
      421 ttcgatcggc gtcgtctatg cacgccaacc ggtcgagtat cacgttctcg gttctattga
      481 cataaattgc ggccacagtg ttgtggctcc aactgtaatg tgaattctca atcagcagat
      541 ttttcacgtc gtaaaaaaga tgaccggcag tacccagatt tgaaacattc atcttcagac
      601 cggaaatgta aatcagatcg atcggcgcag ttgccgggcc ccagtttcgg ggaaaggcac
      661 tgattcccac ccagccgcct tcgtcaagaa aaatatcgcg catgacgact tcgcggcgtt
      721 gagagtcggg agtcggagcg gcggcattgg cgatattgat ccaggacagg ggcgctccca
      781 atccggtttt tccgaagaac gggcgcaacc cgtaatcaag aaagatcgaa ttcgagatgg
      841 tgaacttttt ccattcaacg ttttcaacta caggagcgta tgcaccgctg ttcgcagtac
      901 atccgagaaa caccgtgagc tcaatggaca attcactgcg gactgctctg acgaggttgc
      961 ctcctccgaa gccgggtcgt aatccgaacg tgctgatgcc gtaaaactcg cagtgccgga
     1021 tcgtcgcttt atcaatgtcg ctcaaataaa gcgtgacgaa agcatcggta aaagcggtgg
     1081 gccgaccggt gaagcccaca tgttcgatcg tgaattccgc gaggttgctg agcctgattg
     1141 catcgtcttt gtcgcccgtc gcggggatga attccgagac gagatccaaa ccggcagcca
     1201 gaaactgacc attggcatgc ggcgaagccg gcatggtatc ggagggcaca ccctgaattt
     1261 tcacctgtgc ggagttgata ctggagaagt ctctttcgac tggtgtcgca ataagatatt
     1321 gaccggcagg aatgagcaac gtgccgcctc ccgcatcggc gagcgcgtcg agtgctcgct
     1381 gtaaggctgg accatcgtca gtaacgccgt cgccgactgg atcaaaatcc gacagactca
     1441 agatcgtttc gccggctggg gaactttgcc ccatcgctcg agaaactttt gtcttagcac
     1501 tgcctacaat cccgctcaag acaatcagaa tgatgcttgc aactgccagg cacggctgtc
     1561 gcagaaaccg ttcattcaag ttcatattta gagtgaatct ctacctgggc gagcaaagtc
     1621 ggggagaaaa tggataaaag ttggaccaag ctcagtcagc ctggtccatt acaagataca
     1681 gggcggaaaa actatgcgca acaacttttt acgcattggg aatgagtcga agagtgacgc
     1741 tgaggagaga tatcgacacc acgacaatat atgactagct aagaagtgcg gacgcgcgct
     1801 tttttgttgg agggttgcaa cttacagcca aataaggcat cgtagtaccc gttaatcctt
     1861 ctctcgcgca tcgacggtga tgcgccctgc gccgcaaata tcagcgccgg acgtttggat
     1921 ggttcgagag caggcttgtt tcaaactctg gaagccgctc attaagagca tccgaacctg
     1981 gtccttcgac cggcaaccgt ttcgccgaag cccatccgat tcgcagcttc tttttcttcc
     2041 caaaggggaa aggtatatct gtaatgtcaa cgaaacttta tgttggaaat ctcgctttcc
     2101 aaaccaccag tgaggatctg caacagctct ttgctcaggc gggcacagtt caatcagcaa
     2161 acgtggtcga agaccgcgag actgggcgct cccgtggatt cgggtttgtc gagatggcca
     2221 ccaacgccga ggctgtcgca gccattgacc agttcaatgg taaggagatg gccggccgcg
     2281 cgttgaaagt caatgaagcc aagcctcgcg aaaatcgtaa tgcctcgacc ggacgcaact
     2341 ttggtggtgg gaaccgcggc ggttttcgtg gtggtaacca cggtggtaat aacggcggtg
     2401 gcaaccgtcg cgagcctcgc tggtaaatcc ctcatcactc tcgggcagtt tgtcttctat
     2461 tacttgcgac cagaagatgc gcaaggatag acaagactgc ctgcacaaca atcgttacgt
     2521 tcggagaacg taaataaatt aaggaacact atgaattttt ctcaactggg actcgcgccc
     2581 gcacaagtga gcgcgtgcga gtctctcgga tacaaaactc ctacaccaat ccaacgtcag
     2641 gcgataccgg taattcttag cggtcgcgac gtgatcggct gtgccgaaac cggtaccggt
     2701 aagaccgccg catttttact tccgatcatt cagaggctaa tggaaagtgc tcgacccggc
     2761 ttgcgcgttc tcgtgcttgc gccgacgcgt gagctggctc ttcagattca ggaaaactac
     2821 actgcgctaa acaacgtaaa gaccaaccgt agcgtgcttg cgattggcgg cgcg
//
LOCUS       DASUTG010000442         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_68101, whole genome shotgun sequence.
ACCESSION   DASUTG010000442 DASUTG010000000
VERSION     DASUTG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68101"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VF089_10120"
     CDS             complement(<1..163)
                     /locus_tag="VF089_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX6174361.1"
                     /translation="MADKYDIIIVGGGSAGLVAANRLTEDPNRKVLLLEAGPDPQPLP
                     ELVAEASNQT"
     gene            complement(188..646)
                     /locus_tag="VF089_10125"
     CDS             complement(188..646)
                     /locus_tag="VF089_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6174362.1"
                     /translation="MIFEGKIDLDVPAAKTWDFLIDINKFSSCLPGLQEVKQIDDKTF
                     DGVIAANVGPISGKFSFRAMIVESKPPEQMVVKTEGTDSVTKSTVNADMLVDLQQPAA
                     NETMMNYRADIKIKGRLGILGDMVLRATATLILQEFTRRLRQGLGATARA"
     gene            complement(941..>3327)
                     /locus_tag="VF089_10130"
     CDS             complement(941..>3327)
                     /locus_tag="VF089_10130"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6174363.1"
                     /translation="VADRSVLPYILFNNPRLRLVAFLITCLLKFISGGRAVAYLYARI
                     NSLKYGGSLMAASKQVIGNPTPRVEGELKVSGKAKYAVDVTLPGMLWGKLYRSPNASG
                     RIKRIDISKAEALPGVHAVVTGKDCTGLRIGRRLYDMPILADGEVRFIGEKVAAVAAD
                     NESIAEEALNLIEVEFEETEPLLDPREAMEAGAKLLHPDVMNYKGLPKALEAPSNEFV
                     YVTWKKGEIETGFRQADIIVENTFTTPVVHHAYIEPHSCVANVAPDGSAEIWACSKVP
                     YAIREQVATALQIEQEKLVINPCYIGGDFGGKGDFMDVAVAYLLSKKAGRPVKLVMDY
                     DEEFVAGNPRHASIIRVKTGVKKDGTIVAHHMDFVFDSGAYGAFKPNAFLNGPHGCAG
                     PYNIPHCLIEEHMVYTNKIPCGHMRSPGDPQGFFANESQMDLVAKRLGMDPLKFRQKN
                     LMHDGDVDPIGEEVAYIKTEETLAKALEDAGFNRPKPKNVGRGMAFVQWLANGGFGTV
                     ALTLEENGTVTISSAMLDQGAGTYTVLAEIVAEELKIPVSQIRTQSLDTDQGTKDSGV
                     GGSRATRVYGNAGYEAALKAVEAIKQAAAEQLGTSPDQITLAKGAALHPRMERRLTYA
                     DLVKAKGAPIKVEATYNNNKKVHEASMCVQIAEVEVDPETGQVQLKKLTSTHNTGTVL
                     NPLMHQGQIEGGAMTGIGYALMEHVIINDGKVATANFGEYKIPTIADVPVFKSSVSER
                     PKGPGPYDSMPIGETANVPTAAAIANAVEDACGVRIKSLPITAEKIYEALHGGK"
BASE COUNT          693 a          959 c          874 g          801 t
ORIGIN      
        1 gagtttgatt ggaagcctcg gcgacaagct ccggcaacgg ctgcgggtca ggcccggctt
       61 cgagcaacaa gactttgcga ttgggatctt ctgtaagccg gttcgcggct accaggccgg
      121 cggagcctcc gccgacgata ataatgtcat acttatctgc catcgaaact cttcctccgt
      181 cgcgcgctca cgctctggct gtcgcgccca gaccctggcg taggcgccta gtaaactctt
      241 gcaaaattaa tgttgctgtg gcgcgcagca ccatatcgcc gagaattccg agccgtcctt
      301 tgattttgat gtcggcacgg tagttcatca tcgtctcatt cgcggcaggc tgctgcaaat
      361 ccacgagcat atccgcattc accgtgctct tggtcacgga atcggtgccc tcggtcttca
      421 ccaccatctg ttcgggtggc ttactttcaa caatcattgc acgaaaagaa aacttgcccg
      481 agatcggccc aacattggcg gcgatcaccc cgtcaaaggt tttgtcatcg atttgtttga
      541 cctcctgtag cccgggcagg cacgacgaga atttattgat atcgatcaag aagtcccacg
      601 ttttcgccgc aggcacatcc agatcgatct ttccttcaaa aatcatcctt gctcctcaat
      661 caaatcagcg ccgatcttat gtgactaaaa aactcctgac aaggggatga agcgcaagga
      721 tgaaagcgga aggaaaaatc cgagacctgt gcgaaaacag ccgtacccgg gacttaggac
      781 atcctcgttg atttgaagct cgtcctagtc cggagcacga ttacggagca cgaacacgcc
      841 aaaggtggat taaggcgtcc ctagataaga ctacttaagc acccgcgata tcggcggggc
      901 gctgacgcgg aaatgagcgt tgcctgaagc cgtaccagaa ctacttaccg ccgtgcagcg
      961 cctcgtaaat cttctctgca gtaatcggca ggctcttgat gcgcacgccg caagcgtctt
     1021 ccacggcatt ggcgatggcc gcggcagtgg gaacgttggc ggtttcgccg atgggcatgc
     1081 tgtcgtacgg cccggggccc ttcggtcgtt cactcaccga gcttttaaac accggtacat
     1141 ccgcgatggt gggaattttg tactcgccaa aatttgcggt cgctacctta ccgtcattga
     1201 tgatgacgtg ttccattagc gcgtaaccga ttccggtcat ggcgccgcct tcgatttgcc
     1261 cttgatgcat caacggattg agcacagtgc cggtgttatg ggtagaagta agttttttca
     1321 gctgcacttg cccggtttcc ggatccacct ctacctcggc gatctgcacg cacatggaag
     1381 cttcatgaac ttttttgttg ttgttgtaag ttgcttcgac cttaatcggc gcccccttgg
     1441 ctttgactaa atcggcgtat gtcagccggc gttccatacg ggggtgcaag gcagcgcctt
     1501 tggcgagcgt gatctgatcg ggcgatgttc ccaattgctc ggccgcagcc tgcttgatgg
     1561 cttccactgc cttaagagcg gcctcataac cggcgttgcc atagacccgc gtggctcggc
     1621 taccgccgac gccgctgtcc ttagttccct ggtcggtgtc caacgattgg gtcctgatct
     1681 gactgacagg aattttgagc tcttccgcga caatctcagc caggaccgtg taggtgccgg
     1741 cgccttggtc gagcatggcg gaagaaattg tcaccgtacc gttctcttcg agagtcaaag
     1801 cgaccgttcc gaaaccgcca ttggcgagcc attgcacaaa cgccatgccg cggccaacgt
     1861 tctttggctt cggtcgatta aaacccgcat cctccaacgc cttggcgagc gtttcttcgg
     1921 tcttgatata ggcgacttcc tcgcctatgg gatcgacatc gccatcgtgc atcagatttt
     1981 tctgcctgaa cttaagcggg tccatgccga gccgtttggc aacgagatcc atctggcttt
     2041 cattagcgaa gaatccctgc ggatcgccgg gagagcgcat gtggccgcac ggaatcttgt
     2101 tggtgtagac catgtgctcc tcgattaaac agtgcgggat gttgtaaggg cccgcgcaac
     2161 cgtgcggtcc attgagaaag gcgtttggtt taaaagcgcc ataggcgccg ctgtcgaaaa
     2221 cgaagtccat atggtgcgca acgatggttc cgtctttctt gacaccggtc ttcacccgga
     2281 tgattgaggc gtgtcgagga tttccggcaa cgaactcctc gtcatagtcc atcactaact
     2341 tcaccggccg tccggctttt ttcgacagca gataggctac ggcgacatcc ataaagtcgc
     2401 cttttccgcc gaaatccccg ccgatgtaac acggattgat caccagtttt tcctgctcga
     2461 tctgcagcgc agtggcgacc tgctcgcgga tggcgtacgg aaccttgctg cacgcccaga
     2521 tttctgcaga gccatccggc gcgacattgg cgacacatga atgaggttct atataggcgt
     2581 gatgcaccac cggcgtggtg aatgtgttct cgacgatgat atcggcctgg cggaaaccag
     2641 tttcaatttc gcccttcttc caggtcacat aaacgaactc attggacggg gcctctagcg
     2701 ccttaggcaa tcctttgtag ttcatgacat ccgggtgcaa taactttgcg ccggcctcca
     2761 tggcctcgcg cggatccaaa agcggttcgg tctcttcgaa ttcgacttcg ataaggttga
     2821 gcgcctcctc ggcgatgctc tcattgtctg ccgccacggc ggcaactttc tcaccgatga
     2881 accgcacttc gccgtctgcc aggattggca tatcgtagag ccgtcgtccg atccgcagcc
     2941 cagtgcagtc ttttccggtc accaccgcgt gcacgccggg gagcgcttct gccttgctga
     3001 tatcaatccg ttttatcctc ccggatgcgt tcgggcttct atagagcttg ccccacaaca
     3061 ttcccggcag ggttacatcc accgcatact tggccttgcc gctgactttt agctcgccct
     3121 caaccctcgg agttggattg cctatgacct gcttcgacgc tgccatcaag ctacctccat
     3181 atttcaatga attgatccta gcataaagat aagctaccgc acggccgccg ctgatgaact
     3241 tgagtaaaca ggtgattaaa aaagctacca ggcggaggcg tgggttattg aacagaatgt
     3301 agggtaaaac agaacgatcc gcaacta
//
LOCUS       DASUTG010000443         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1402610, whole genome shotgun sequence.
ACCESSION   DASUTG010000443 DASUTG010000000
VERSION     DASUTG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402610"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF089_10135"
     CDS             complement(<1..447)
                     /locus_tag="VF089_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6174364.1"
                     /translation="MKALKRIIVGHDLRAGGEIAVQSAAALARRSDAALRLVHVIEPL
                     DPYQRISHPLTSPYPVEEIAQKVGVSLQAVLAGAELAGLEAEYEVRTGSPFVELILAQ
                     RAWFADMITVGGASQPEEPSASTSERLMRKAGVPIMVAKSRLSAEAK"
     gene            787..>2391
                     /gene="treZ"
                     /locus_tag="VF089_10140"
     CDS             787..>2391
                     /gene="treZ"
                     /locus_tag="VF089_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX6174365.1"
                     /translation="MKSSGGSLHPVSFGSQYYTNAATGARCLAGNRCSFAVWAKNAST
                     VDVRLLDSSERIVPLVNVGDGYFRAEVENVGPGALYFYRLNQATERPDPASRFQPEGV
                     HGPSQIIDQSFTWNDDHWFGLPLHKYLIYELHVGAYTEEGTFDAIIPHLAELSELGVT
                     AIELMPVAQFPGERNWGYDGAYPFAVQNSYGGPAGLKRLVDACHQQGLAVILDVVYNH
                     LGPEGNYLADFAPYFTARYKTPWGLALNLDGAGSDHVRRFFLDNAHYWITEFHVDALR
                     LDAADQIFDYSPTHFLAELSMEIHELRKELNRQIFLFPETAANDVRYIKSRELGGYNL
                     DAQWNDEFHHALRALLTEERTGYYRDYGEFHQLVKAYTEGFVYSGEYSKYRDRRHGSS
                     TRDIPAERFVVFSQNHDQVGNRMLGERLTQLVSFEKLKLAAGSVLLSPFIPLIFMGEE
                     YGEEASFQYFISHSDPELIEAVRKGRREEFAAFEWNLEPPDPQDQATFHRAKLNHQLR
                     HDGRHRVLREFYRELIRLRKALAPLAR"
BASE COUNT          531 a          679 c          621 g          560 t
ORIGIN      
        1 tttagcttca gcgctgaggc ggctcttcgc caccattata ggaacgccgg ctttgcgcat
       61 aagccgctca ctggtactgg cagaaggctc ttcaggctgg gaggcgccgc caaccgtaat
      121 catgtcagcg aaccaggcgc gctgcgcaag gatgagctcg acaaaggggc tccccgtgcg
      181 aacctcatat tccgcttcca atccggcaag ctccgcgccc gccaatacgg cttgcaggct
      241 tactccaacc ttctgcgcga tttcctcaac cgggtaagga gaagtcaacg gatgcgagat
      301 cctctgatag ggatccagtg gttcgataac atgcaccagt cgcagcgcag catcagatcg
      361 tctggcaagt gccgctgccg actgtacggc tatctctcca ccagccctga ggtcatgacc
      421 cacaatgatt cttttgagcg ctttcataca aaccctcttt tctgaacgac agttgtccgt
      481 tggaaatttc tgcaatcacg atcttattat cagtgaagca acccacatgc cctttggatt
      541 ctttagtata ccgattattt aagcttccag tcaggcgtcc ttaaagttat cactaaaggt
      601 cacaacgata agggcagcaa cgactcacga actagcgaat atgtttaccg tactcagtgt
      661 tgtttgatgc ccctttgagt tagcaaggct taccaagtac attcaacaga tgttcggtaa
      721 acggaagctc gcgatccgtg gtgattcaca ctgcggctgc gagattaaaa tctatcggta
      781 tgaatcatga aaagctcggg cggatcactt catcccgttt cttttggctc tcagtattac
      841 actaatgctg caaccggtgc gcgctgctta gccggtaacc gctgctcgtt tgcggtttgg
      901 gccaagaatg catccacagt cgacgtgcgg cttctcgatt cgtccgaaag gattgtccct
      961 cttgtgaacg ttggggacgg ctactttcgc gccgaggtgg aaaatgtcgg gccgggcgca
     1021 ctgtacttct accggctaaa tcaagcaacc gaacggcctg accctgcctc gcggttccaa
     1081 cccgagggcg tacatggccc atcgcaaata atcgatcagt cttttacctg gaacgacgac
     1141 cactggttcg gcttgccgtt acacaaatat ctcatctacg agcttcatgt cggcgcctac
     1201 accgaagagg gaactttcga tgcgatcatt cctcatctag cggagctcag cgagttgggg
     1261 gtcaccgcca ttgagctgat gccggtagcg caatttcctg gcgagcgaaa ctggggctac
     1321 gatggcgcct atccatttgc ggtgcagaat tcatatggcg gcccggccgg cctaaaacgc
     1381 ctggtcgatg cctgtcatca gcaggggttg gccgttatcc ttgacgtcgt atacaaccat
     1441 ctcgggccgg aaggaaatta cctggcggat ttcgctccct atttcaccgc gcgctataaa
     1501 accccgtggg gtttggcgct caatctagac ggcgccggga gcgatcacgt tcgtcgcttc
     1561 tttcttgaca acgcgcacta ctggattacg gaatttcacg tcgacgcgct gcgcctggac
     1621 gcggccgatc agatatttga ttactcgccg acgcattttc tcgcagagct cagcatggaa
     1681 attcacgagc tgcgcaagga gctcaaccgg caaatcttcc tatttcccga gaccgcggcc
     1741 aacgacgtcc gctacatcaa atctcgcgag ctcggcggct acaacctcga cgctcaatgg
     1801 aatgatgagt ttcatcatgc gctgcgcgct ctcttgaccg aggagaggac cggttactat
     1861 cgcgactacg gggagtttca tcagctggtc aaagcctata ccgagggttt tgtttactcc
     1921 ggcgaatatt cgaaataccg tgatcgcagg cacggcagct cgactcggga catacccgcc
     1981 gagcgcttcg tggtcttttc acaaaatcat gatcaagtcg gtaaccgcat gttaggagag
     2041 cgactgaccc aactggtctc cttcgaaaag cttaaactgg ctgccgggtc ggtattgctg
     2101 tcgcccttta tcccgctgat tttcatgggc gaagaatacg gtgaagaggc gtcgtttcaa
     2161 tattttatca gccattcgga tcccgagctc attgaagccg tgcgcaaagg ccggcgcgaa
     2221 gagttcgccg ctttcgaatg gaatctcgaa ccgccggatc cgcaagacca agcgaccttc
     2281 caccgcgcca agttgaatca tcaattgcgc cacgatgggc gccaccgagt cctgcgagaa
     2341 ttctatcgtg agttgattcg actgcgtaaa gcgctggcgc cgctggcgcg a
//
LOCUS       DASUTG010000444         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1402658, whole genome shotgun sequence.
ACCESSION   DASUTG010000444 DASUTG010000000
VERSION     DASUTG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402658"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF089_10145"
     CDS             <1..250
                     /locus_tag="VF089_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174366.1"
                     /translation="TTDALFQETPELIAQLTQQNVSSIDMVTSVFLTIAQLRKKKAGA
                     ILAVSDECLHGKFGFRDPLFFAAEERIIAVAREALKYL"
     gene            274..1785
                     /locus_tag="VF089_10150"
     CDS             274..1785
                     /locus_tag="VF089_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174367.1"
                     /translation="MTSVQSPLFEPVLWEGDGFRILDETSLPEQIRYISVRDVSQAIN
                     AVREMKTRAFGQVLTFLYSGALLAQHYRRKEISPLRESIDQMSDEFCRARPTFDFRGL
                     GQYFHEWLEKLPGTMSAGEWISGRACDFAAQIIRARGARAKRAAELLPDRARVLTHCN
                     MSGELLATAQHCAAMGKEFAVIATETRPYLQGARLTAWELARGGVQVSLIPDCAVAQV
                     LARGEVDAVIVGSDRAAQNGDIINKVGTYPLAVMAKRYGVPFHVLVQDPRSLVQGIDV
                     PIEERPGAELLSFQGQPLMAEDSIAVRYPAFDVTPAALISRLVGFDEIYTPESFRRKY
                     QQTVAVPQSSKDESKYLLIYGVPPLDQCDYLISALKAEQASGLLIPEMRPEMWGVHAV
                     APALLQQNAPTTLISDNMMGILFSRREIRKLYLYYSRLDQQGVSGICGSLLALGLARA
                     HDVPVELLASDSVKRATVDSDVSTFMGKSICPRGASIFPVQSEVMPWELFEKS"
     gene            1818..2798
                     /locus_tag="VF089_10155"
     CDS             1818..2798
                     /locus_tag="VF089_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6174368.1"
                     /translation="MIKCGKLLFKRHLVSGWGGNLSCRLGENELLITGRHAPLGFLTT
                     ADLVRIDGAGRSLNRDQLASSETPLHLAVYAATGAQAIVHVHPPMVLAFSLSHDHFVP
                     ISFEEKYTIGEVPIVAQDTPTVTKPENLADQLKYRPVVILKGHGTVAIGKDFQEAFLF
                     TDLLEEAVRCRFFTQLRSESNVSIADQATQQKVLAEAQTGKSYVLFSKEHMSALVECA
                     NHDSQFRSQGKETGLSTSLTLLLEEKDTAWTVRFECGQITQLDSDDRGEFMISGPEEW
                     WSAVFQARIDPFLATQQGKLKLKRGELAQLARWYKPFERAFSLWRTVLIQ"
     gene            2774..>4189
                     /locus_tag="VF089_10160"
     CDS             2774..>4189
                     /locus_tag="VF089_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6174369.1"
                     /translation="MANRIDSVGFFAAELLDQAAREHPNKTAIIDKEEELSFSVLNGR
                     VHALAGHLHSEGIKQSERVGVLLPNCAAIPLIYFASQKIGAVSVLLDARLRGKELQDV
                     LRDADLKLLVIHQQLISEVAPVLKELTPLPVWIVQGRGEEAFEYRYVAPPQSALLPQL
                     NAEDDALILYTSGTTGEPKGVVLSYRNLAQYPRVMTQMGITDPSTIRGCILPMSHIVG
                     PLVCNEVADKGFTLVIFDQINPVTLLDGIQRHRISVFEGVPIVFQLLLGVRDLSSYDT
                     RSVKIAAMMGTSVPLPLLRAFHSAQPHIAVIQGYGLTETSPMITLVEPEKAEAKMGSI
                     GRAVPGVEVKIIDKSGREMPLDEPGEIITKGPHVMKGYFRKPDATAARIRDGWLYTGD
                     VGKRDADGYFVHLGRLDDMIITSGLNVYPAEVENLIYSFPNVQETIVFAIPDSKRGQV
                     IGAAVVPRPGKTVLPKELLSFL"
BASE COUNT          972 a         1065 c         1171 g          981 t
ORIGIN      
        1 taccactgac gctttgtttc aagaaacgcc ggagttgatc gcccaactca cacaacaaaa
       61 tgtctcgagc atcgacatgg tgacctcggt ttttttgacg atcgctcaac ttcgaaagaa
      121 aaaagcgggt gccattcttg ctgtctcgga tgaatgttta cacggaaaat tcggctttcg
      181 tgacccgttg tttttcgccg ctgaagagag aatcatcgcg gttgcccgcg aagccctcaa
      241 gtacctttag aaccgatgca actgtagata tttatgacct cggtacaaag cccgcttttt
      301 gagccggttc tatgggaagg ggacggtttt cgcattctcg atgagacttc gcttcccgag
      361 cagatccgat atatcagcgt cagagatgtc tcgcaggcga tcaatgcggt acgtgaaatg
      421 aaaacgcgtg cgtttggcca agttctcacg ttcctttata gcggtgcact cctggcgcaa
      481 cactatcgcc gaaaggaaat atccccgctg cgagagtcga tcgatcaaat gagcgatgag
      541 ttctgccggg cacggccgac gttcgatttc cgcggcctag gtcaatattt tcacgagtgg
      601 ctcgaaaagc tgccgggaac catgagtgcc ggtgaatgga ttagcgggcg ggcttgtgat
      661 ttcgccgcgc agatcattcg tgcccggggc gcaagagcaa aacgcgctgc cgagcttttg
      721 ccggatcggg cgcgagttct gacccactgc aacatgagtg gggaactctt ggcaacagcg
      781 cagcattgcg cggcgatggg caaagagttc gccgtcatcg ccaccgaaac ccgaccctac
      841 ctgcaaggcg cgagactgac cgcgtgggag ctcgcccgag gaggcgttca ggtgtcattg
      901 attcccgatt gcgctgtcgc ccaggtgctg gcgagaggcg aagtcgatgc agttatagtc
      961 ggttccgatc gtgcggcaca gaatggcgat atcatcaata aagtcggcac ttatccactg
     1021 gccgtaatgg ccaagcgata cggagttcca tttcacgttc tggttcagga tccgcgttcc
     1081 ttagttcagg ggattgatgt gccgatcgaa gaaaggccgg gcgcagagct gctaagcttt
     1141 caaggacaac cattgatggc ggaggatagt attgcggtgc gctaccctgc ttttgatgtc
     1201 accccggcgg cgctgattag tcgtcttgtt ggatttgacg agatctatac gcctgaaagt
     1261 ttccgccgaa agtaccagca gactgtcgcg gtaccgcagt cgagtaagga tgaaagcaag
     1321 tacttgctta tctacggggt gcccccttta gaccaatgcg actacttaat aagcgctttg
     1381 aaagcggagc aagccagcgg tttgttaata ccggagatgc ggccggagat gtggggtgtt
     1441 catgccgtcg cgcccgctct cctacagcag aatgcgccaa cgactctgat ttctgacaac
     1501 atgatgggaa ttcttttttc gcgcagggaa atccgtaagc tctacctcta ttatagccgg
     1561 ctcgaccagc agggagtcag tggcatctgt ggctcactgt tggcgcttgg tttggcgcgc
     1621 gcccatgatg taccggttga actgctggcc tctgattcag ttaaacgagc gaccgtcgat
     1681 agcgacgtct cgaccttcat gggaaaaagc atttgtcctc gtggagcctc tatatttccg
     1741 gtccaaagcg aagtcatgcc ctgggagttg ttcgagaagt cttaagatca ggaaaaatga
     1801 aaaagctcga agaagagatg atcaaatgcg gcaagctgct gttcaagcgg catctagtca
     1861 gcggctgggg cggaaatcta agttgccggc ttggagagaa cgaacttttg atcacggggc
     1921 ggcatgcgcc gctaggtttt ttgacaacgg cggatttggt gcggattgat ggcgccggca
     1981 ggtcgcttaa ccgcgaccag ctcgcatcga gcgaaacgcc cctgcatctg gccgtgtatg
     2041 cagccaccgg ggcacaagcc atcgttcatg ttcatccacc gatggtactg gcattctcat
     2101 tgagccatga tcattttgtt ccaatcagtt tcgaggagaa gtatacaatt ggcgaagttc
     2161 caatagtcgc ccaggataca cccacggtaa caaagcccga gaatttggca gaccaattaa
     2221 agtaccgtcc ggtcgtcatt ctcaaggggc acggcacggt ggctatcggc aaggattttc
     2281 aagaggcttt tttgtttacc gatctactgg aagaagccgt gcgctgccgg ttcttcacgc
     2341 agcttcgaag tgaatcgaac gtttcgattg cggatcaagc aacacaacaa aaagtcctgg
     2401 ccgaagcaca aactggtaaa tcttatgtgt tgttctcgaa agagcacatg tccgccttgg
     2461 tcgagtgcgc caaccatgat tctcaattcc gttcgcaagg gaaagagacg ggcctctcta
     2521 catccctgac acttttactt gaagaaaagg acaccgcctg gaccgtccgt tttgaatgtg
     2581 gccagatcac gcagcttgat tcggatgatc gcggtgagtt tatgatcagc ggtccagaag
     2641 agtggtggag cgcagtgttt caagcccgga tcgatccttt tcttgcaact caacagggca
     2701 aattaaaact caagcgcggc gagctggcgc agctcgcgcg ctggtacaaa ccatttgaga
     2761 gggccttttc gctgtggcga accgtattga ttcagtaggt ttttttgctg cggagctgct
     2821 cgatcaagca gcacgagagc atccaaacaa aaccgccatc attgacaagg aagaagagct
     2881 gagtttttcg gttctcaacg ggcgggtgca tgctctagcc ggtcacttgc atagcgaagg
     2941 catcaaacag agcgagcggg tgggagtgct attaccaaac tgcgcggcaa ttccgttgat
     3001 ttattttgct agtcagaaaa tcggcgcggt cagcgtgctc cttgatgccc gtttaagggg
     3061 gaaggagtta caggacgtct tgcgcgatgc ggatctcaag ctcctggtta tccatcagca
     3121 gctgatctct gaagtggccc ctgtactaaa agagctcacc ccgttgccag tatggatcgt
     3181 tcaaggccgg ggtgaagagg catttgaata tcgctacgtg gcaccgcctc aatcggcgct
     3241 cctgccgcag ctgaacgctg aagacgacgc attgattctt tatacctcgg gaaccacggg
     3301 cgaacccaaa ggcgtagtcc tcagttaccg taacctggcg cagtatccac gtgtcatgac
     3361 tcagatggga atcaccgatc cgagcacgat ccgaggctgc atcctaccga tgtcgcatat
     3421 cgtagggcct ttggtttgca acgaagtagc cgataaaggg tttactctgg ttattttcga
     3481 tcaaatcaat ccagtcacct tgttggacgg cattcagagg catcgcatta gtgttttcga
     3541 aggcgtgccg attgtctttc agcttctact aggagtgcgc gacctttcaa gctatgacac
     3601 ccgcagcgtg aagatcgccg caatgatggg caccagcgtt ccgttgccgc tcctgcgggc
     3661 atttcattca gctcagccgc acatcgcagt gatccagggt tacggcttga ctgagacttc
     3721 gccgatgatc acgttggttg aaccggaaaa ggccgaagcc aagatgggaa gcattggccg
     3781 tgcagtaccc ggagtcgaag ttaaaatcat cgataagtcg ggcagggaaa tgccgctgga
     3841 tgaaccggga gaaatcatta ctaaaggtcc tcacgtgatg aaagggtatt ttcgcaagcc
     3901 agatgccacg gcagcgcgca ttcgtgatgg ctggctttac accggtgacg tcggtaaacg
     3961 cgatgcggac ggttacttcg tccatctcgg tcgacttgac gatatgatca tcactagcgg
     4021 tcttaacgtc taccccgcgg aggtggagaa cctgatctac agctttccca acgtgcaaga
     4081 aaccatcgtt tttgccatcc ctgattcgaa gcgcgggcag gtgatcggtg ctgctgtagt
     4141 gcctcggcca ggcaaaacgg ttcttccaaa ggagttgctc tctttcctg
//
LOCUS       DASUTG010000445         4702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1698872, whole genome shotgun sequence.
ACCESSION   DASUTG010000445 DASUTG010000000
VERSION     DASUTG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698872"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VF089_10165"
     CDS             complement(<1..298)
                     /locus_tag="VF089_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174370.1"
                     /translation="MEHSRRSFLKSIGLGFVALGLPPTFLVRAAGAEQKSQGKVLVVV
                     FQRGGMDGLNVVVPFKDPAYYQLRSNIAVSPPAASDDSAIDLDGFYGLHPALAPL"
     gene            complement(300..1709)
                     /locus_tag="VF089_10170"
     CDS             complement(300..1709)
                     /locus_tag="VF089_10170"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX6174371.1"
                     /translation="MAPSLTQQQKFVHFLNRTSFGPTREELEKLEQLGITAYLDQQLS
                     PEQIPDSVVADTVAELKTMRLSSRELLELYPQASVAQAKGMDMAQMQGPRQVILELQL
                     ARLIRAVYSRRQLYEVMVDFWSNHFNIFAGKGADRWLSTAYDRDTIRPHALGAFKNLL
                     TATAQSPAMLFYLDNWMSVSPESPAAQRGPNNRRRGINENYAREVLELHTLGVDGGYT
                     QKDIQEVARCFTGWTIERPRGQGEFRFEQRFHDNGAKTVLGTYIAAGGGMEDGVKVID
                     ILADHPATAKFIATKLARRFVADDPPVAVVSKASAVFQQSGGNIVSVVRAIIDSPEFF
                     SGQFYHGKVKKPLEYVASALRVTGAETRITHSLLRYLNRMGEPLFLAQPPTGHPDLGT
                     SWISPDMLLTRMNFVSDLVGNRLAGSRVSKDSVGDPEAIIRLIAPDSISSSTRAALNE
                     TKGAESVALLLAAPEFQRR"
     gene            complement(1713..2327)
                     /locus_tag="VF089_10175"
     CDS             complement(1713..2327)
                     /locus_tag="VF089_10175"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEX6174372.1"
                     /translation="MKSATICLVLLLSVLLAPYPVWSQSRDGGESSAQTNREADRWQR
                     MTPQQKQELRDRWKSLPPEEKAEMRKKFERWRSMSAEEKASARKNFERWQQLSPEDRE
                     RLRERWQRWRELPPESREALKRRLERFRELPPETRQELREKARERFEKMSPEEKQQRR
                     ERFRQRMERLSPEEKRAMREKFRERKQREAEEPGTLRNESREKD"
     gene            complement(2467..2967)
                     /locus_tag="VF089_10180"
     CDS             complement(2467..2967)
                     /locus_tag="VF089_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6174373.1"
                     /translation="MSTVCNEFEQDLVLYHYGDLSAEDRDRLATHLRACPGCATYLEE
                     LAALLPLTMNTDEPGEEFWQNYSREMRHKLADLQEKKSWWHALQTALRLWTLPALAGT
                     AVVALAITLNTANKTTPINDVPAADPALMEVLPMAENLELFNNMDVLENIEVLEIMEG
                     SANEAA"
     gene            complement(2976..3611)
                     /locus_tag="VF089_10185"
     CDS             complement(2976..3611)
                     /locus_tag="VF089_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6174374.1"
                     /translation="MAFALQGCALSLDGAFEQCMETAPDKPRDEALVILAQKGDSKAF
                     EELIERYKQKAFRIAFDFSRDRDEAKDLSQEAFLRAYMHLKNFDGRSGFYTWFYRILV
                     NICLDYRRKTKRASAEEFNETVEGQVEPSHPAAHSVSPDQHAIAAQMSRQVDAALDAL
                     PPRQRMAFVLKNHQGLSIREIAEMMETAEGTVKVHLHRAVTALRVRLAEFA"
     gene            3776..3916
                     /locus_tag="VF089_10190"
     CDS             3776..3916
                     /locus_tag="VF089_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174375.1"
                     /translation="MTRVRAAYNRDYGQGGDCLNRRTLNLAVRYYGSVKQVRRACAKH
                     VY"
     gene            4345..>4702
                     /locus_tag="VF089_10195"
     CDS             4345..>4702
                     /locus_tag="VF089_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174376.1"
                     /translation="MVYPSSALRTVAGLALVGAVFFLPTVCMAQGSELEALRAAVKDM
                     QQDLQKALSRIEQLEKEKTADSARLGQVETSIKTVQSAPSILNPAIGLVIDATAEHRD
                     KAGGDFNFRAAELGISA"
BASE COUNT          995 a         1301 c         1261 g         1145 t
ORIGIN      
        1 tgagcggcgc aagtgccgga tgtaacccat agaagccatc gagatcgatg gcactgtcgt
       61 cgcttgcggc tggcggagac acggcgatgt tggaacgcaa ttgataatag gccggatctt
      121 taaacggcac gaccacattt aaaccatcca tgccgccgcg ctggaacacc acgacgagga
      181 ctttgccctg gctcttttgc tccgcacccg ccgcgcgaac caggaaagtc ggtggcaatc
      241 ccaaggcaac aaaaccgagc ccgatcgatt tgagaaatga tcgtcgtgaa tgttccatat
      301 catctcctct gaaattccgg tgcagcaagc agaagagcaa ccgattcggc acctttggtc
      361 tcgttcaacg ctgcgcgagt cgaggaagaa atcgagtcgg gtgcgatcaa acgaatgatc
      421 gcctccggat cgccaacgga gtctttcgag acgcgcgagc cggccagacg attgcctacc
      481 aaatcgctga cgaaattcat ccgtgtcagc aacatgtccg gactgatcca ggaagtaccc
      541 agatcggggt ggccggtagg tggctgcgcc aggaacagcg gttcgcccat gcggtttaag
      601 tagcgcaaca aagagtgagt aatcctggtc tcggcaccgg taacacgcag tgcgctggcg
      661 acatattcca gcggtttctt taccttgccg tggtaaaact ggccggaaaa gaattccggc
      721 gaatcgatga tcgcacgcac taccgatacg atattgccgc cgctctgttg gaagacggcg
      781 gaggctttgc tgacgaccgc taccggcgga tcgtcagcga caaaacgacg cgctaatttt
      841 gtcgcgatga atttagccgt tgccggatga tctgccagaa tatcgatcac tttcacgccg
      901 tcttccattc cgccgccggc tgcgatgtag gtgccgagga cagtcttggc gccattatca
      961 tgaaagcgct gctcgaagcg aaattcgcct tggccgcgcg gtcgctcgat ggtccagccg
     1021 gtaaaacagc gggcgacctc ctggatatct ttctgcgtgt aaccgccgtc aactccaagc
     1081 gtgtgcagtt ccagcacctc gcgggcatag ttttcgttga tccctcgccg gcgattattc
     1141 ggtccgcgtt gagccgcagg gctctccgga ctgacgctca tccaattgtc caggtaaaaa
     1201 agcatcgccg ggctttgcgc cgttgccgtt agaagatttt taaatgctcc cagagcatgg
     1261 ggtcggatgg tgtcgcggtc atacgcggtg gatagccagc gatccgcgcc cttgccggca
     1321 aatatattga aatgattgct ccagaagtcg accatgactt cataaagttg ccgccggctg
     1381 taaaccgcgc ggatcaatct ggcaagctgc agctccaaaa tcacttgacg cggcccctgc
     1441 atctgcgcca tgtccatgcc tttggcttgc gcgactgaag cttgcggata gagctcaaga
     1501 agctcacggc tgcttaaacg catagttttg agctcagcga ctgtatcggc aacgaccgaa
     1561 tcagggattt gctcggggct gagttgttgg tcgagatatg cggtgatccc caactgctca
     1621 agtttttcga gttcttcacg tgtcgggccg aagcttgtgc gattgagaaa gtgcacaaac
     1681 ttttgctgtt gggtaagcga tggtgccatg atctaatcct tttcccgcga ttcgttgcga
     1741 agagttcctg gttcttctgc ttcacgttgt ttacgctccc gaaacttttc tcgcatggcc
     1801 cgtttttctt ccggcgataa tcgctccatt cgttggcgaa agcgctcgcg ccgctgctgt
     1861 ttttcttccg gagacatttt ttcgaatcgc tcgcgcgcct tctccctgag ctcttgtctg
     1921 gtctcgggcg gtagctcgcg aaagcgctca agccgacgtt tcagagcttc gcggctctcg
     1981 ggcggcagtt cacgccatcg ttgccaccgt tcgcgcaagc gctcgcgatc ctccggggag
     2041 agttgctgcc aacgctcaaa gttcttgcga gccgaggcct tctcctctgc tgacatgctg
     2101 cgccatcgtt cgaacttttt ccgcatttcc gccttctcct cgggcggtag ggatttccag
     2161 cggtcgcgca gttcctgttt ttgctgcggc gtcattcgct gccagcggtc ggcttcacga
     2221 ttggtttgcg ccgaagactc gccgccatct ctcgattgac tccaaaccgg gtagggcgct
     2281 aataggacgc taagcaaaag aacaaggcaa atcgttgctg atttcatttt atcgctttta
     2341 attgtcgcaa tggtttcatc gtgcgcagca tcgctcgatc aggctgtgct acttgttccg
     2401 atggcggctc ggccgtgccc acaaggcgag atcaagcaaa gtcccgtagc ggatcaattt
     2461 tcagatttaa gctgcttcgt tggccgaacc ttccataatt tcgagcacct cgatgttttc
     2521 aaggacgtcc atattgttga ataactccag gttctccgcc atcggcaaaa cttccatgag
     2581 agctggatcc gccgcgggca cgtcgttgat gggggtcgtc ttgttcgcag tatttagcgt
     2641 aatggctaga gcgacaactg cggttcctgc cagagcgggc agtgtccaaa ggcggagggc
     2701 cgtctgcaac gcgtgccacc aggatttttt ttcttgcaga tctgcaagtt tgtgacgcat
     2761 ttcacggctg tagttctgcc aaaactcttc gcccggttca tctgtgttca tcgttagggg
     2821 gagtagagcc gccaattcct ccaggtaagt tgcacaaccc gggcaggcgc gcaggtgagt
     2881 tgcgagccga tcacgatctt cagcgctcag atcaccataa tggtagagca ccagatcttg
     2941 ttcaaactca ttgcataccg tagacataaa tcctcctagg cgaactcagc caatcttact
     3001 ctgagcgccg ttaccgcccg gtgtaaatgg accttaactg tgccctcagc ggtctccatc
     3061 atttcagcaa tttcacgaat cgatagcccc tgatgatttt taagaacgaa tgccatccgt
     3121 tgtctcggcg gcaaagcatc cagagccgcg tccacctggc gtgacatctg ggctgcgatc
     3181 gcgtgctgat ccggtgaaac ggaatgagcg gccggatggc tcggttccac ttgaccctcg
     3241 accgtttcgt tgaactcttc agccgacgcg cgtttggttt tgcgccggta atccaggcag
     3301 atgttgacca gaattcgata gaaccatgta tagaaacctg atcggccatc aaaatttttc
     3361 agatgcatgt aggcgcgaag aaacgcctct tgagacagat ctttggcttc atctcgatcg
     3421 cgagaaaagt caaaagcgat acgaaaagcc ttttgtttgt aacgctcgat cagctcttcg
     3481 aatgctttgg aatcgccctt ctgggctaag atcaccagcg cttcgtcgcg cggcttgtcc
     3541 ggtgcggttt ccatgcactg ttcaaatgca ccatcaagac tcaatgcgca accctgaaga
     3601 gcaaatgcca tacgtacctt cgtattcgga tcgagtgatt ttcttttcgg gttacggtca
     3661 tgccacggag cgatttacct gcgtaagaac gatcggccac gattaagata atttcgtccc
     3721 cgcaactact tagacgcgcc gccgagcgga aggtttacca cggcgagatt ccaaaatgac
     3781 cagagttcga gccgcttaca accgggatta cggtcagggc ggcgattgcc taaaccgcag
     3841 aactctcaat ctcgcggtgc ggtattatgg gagcgtcaag caggtccgca gggcctgcgc
     3901 caagcatgtt tattgacatc ggcaaatcct ttgcgctaag ttcctttcgt gttcgtttct
     3961 ttaacctcca agcctgacat actcccccgt gtcatcagcg tcgcagcgtt gttgtttgtc
     4021 tttttcctgc ctctccattt tcatttctcc gccagcgcag cgatcagtaa agaatgcagc
     4081 tgcattcaag gcactcgaac gcaattagcg ccgacggctg acgcttggca tggtgcgccg
     4141 acataccaga caacttatgt catcacccaa gatgacttac tctgggtcga tgaacgcact
     4201 aagctccaac ccgttcgtgg gccgcctgct tcgctctccc ttagttaaat tttcatccta
     4261 cagtatcgag tttcagtgcc ctccgacttt cgaattccaa gtcagtggga gggctattgg
     4321 tttgtcttgg aagggaggct ggttatggtc tatccgtcta gcgcattgcg aacggttgcc
     4381 ggattggctt tggtgggcgc ggtgtttttt cttccgaccg tatgcatggc ccaaggctcg
     4441 gaattggagg ctttgagagc tgcggtcaag gacatgcaac aggatttgca aaaagccttg
     4501 agccggatcg agcagttgga aaaagagaag accgccgatt cggcgaggct cggtcaggtc
     4561 gaaacatcga taaagacggt gcaaagcgcg ccgtccattc ttaaccccgc cattggcttg
     4621 gttatcgatg ccaccgcgga gcatagggac aaggccggcg gagactttaa cttccgcgcc
     4681 gccgagctgg gaatttccgc tt
//
LOCUS       DASUTG010000446         3922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_217092, whole genome shotgun sequence.
ACCESSION   DASUTG010000446 DASUTG010000000
VERSION     DASUTG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217092"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..512
                     /locus_tag="VF089_10200"
     CDS             105..512
                     /locus_tag="VF089_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174377.1"
                     /translation="MQGLTLAYELIFKLANQIEANAAGAPYPHIRQILQGIAIQKFDS
                     AKKLQTLIEKLGEKPRLTVGEPKTGKNHWERLNFDLQDQIALDDFLFKVELKLGETPE
                     IAKMIEELRSSQRSHRQILSDLVAIADPQATQT"
     gene            complement(569..790)
                     /locus_tag="VF089_10205"
     CDS             complement(569..790)
                     /locus_tag="VF089_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174378.1"
                     /translation="MSYRLTRYNPKPSIEQEREQDLLESVTEEEVIILWRSIKQKGLS
                     EDEAWHAIVTGLALDAAFSSNKRSFQYEH"
     gene            complement(980..1381)
                     /locus_tag="VF089_10210"
     CDS             complement(980..1381)
                     /locus_tag="VF089_10210"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6174379.1"
                     /translation="MPFELLDPTGLESPIGYAHVAKITGGTVVHVAGQAPFDSQGQVV
                     GKGDFVAQFAQVIRNLKTAIEAAGGEPRNYAVLTIYITDLQAYLRNKKPLGSVYKEVF
                     GRHFPAITLVEVKSLYNPDCMVEISGVAVLS"
     gene            complement(1381..2406)
                     /locus_tag="VF089_10215"
     CDS             complement(1381..2406)
                     /locus_tag="VF089_10215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174380.1"
                     /translation="MKIDIEFNSGAQLPMDAIPELARLAEANGFDCAWGGEANNKDPT
                     VMLSAIAAVTTRLKIGSAIYHILGRTPATLALQAVGLDELSRGRFLLGIGSSNPTIAK
                     WHGQVLDQPLGRVREYIEIVQSAIRGEKLDFEGRFFSAHNFKMAFKPSGRAIPIYLAA
                     FGPKMTRLAGRIADGVLINMANPAEIRRIADEVRKGAEEVGKDPAGMQIICKIRCSIA
                     KTSAAARDALSHALTYYALADYYRDLLGRMGFAAEVEAMRNAWKSGGFHSARKLVSDR
                     MFADLPLIAATSAHEIVEHIRPYAAAGATRIILPYVAATDDVKGEMKSFIEQWSSVTT
                     RLTEERS"
     gene            complement(2433..3038)
                     /locus_tag="VF089_10220"
     CDS             complement(2433..3038)
                     /locus_tag="VF089_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6174381.1"
                     /translation="MKRFDCLKLVAAEAKDAVVVSSAGAMTLEWNALRPGDGNFRVRT
                     LGLCSSIALGMALGLPQRKIIALDGDGSLLMNLCSLPTIARMHPSNLLHIVFDNEVYE
                     ASGSKKTATAFGTDLVGVAHAAGIKNSIWANTLSEFGRAISAGITMNALSFIGAKVTT
                     ERTDVSPYPLDEVENKYRFIRYVERTEQVQILKTNLPASYV"
     gene            complement(3074..3598)
                     /locus_tag="VF089_10225"
     CDS             complement(3074..3598)
                     /locus_tag="VF089_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase"
                     /protein_id="HEX6174382.1"
                     /translation="MSAEQAAATKILSGIKQAGINFIATLPDVNLAQLLQAVDEDPDL
                     LHVPVCREEEGIGICAGAYLVGKKCAAIMQNGGFFNSNNAIVTTLLQYHIPLLLMIYY
                     AGDVGDRTFSTSGSMTEPVLQALGIRYYIMRETADAPELIKRAQILAEDSKRPIALLL
                     TKEVLGRRAALKAL"
     gene            3739..>3922
                     /locus_tag="VF089_10230"
     CDS             3739..>3922
                     /locus_tag="VF089_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6174383.1"
                     /translation="MIKLYDFKSSPNCQRVRVVLAEKNLPYETVPVDLRAHAQKTPEF
                     LKLNPYGKVPVITDGDT"
BASE COUNT          974 a         1055 c          959 g          934 t
ORIGIN      
        1 cgtcgagcga agaccggtca atcccaagca cagtgttcga atctataaag aactttctct
       61 ttcccgaagg aaccgataat aactattctc ggcgtgacac acctgtgcaa gggctaactc
      121 ttgcctacga attgattttt aagttggcca atcaaatcga ggcaaacgcc gccggcgcgc
      181 cttacccgca catcagacag atactacagg gaattgcaat ccaaaaattt gacagtgcta
      241 aaaagctaca aaccctgatc gagaaacttg gagaaaagcc ccgactaact gtaggagagc
      301 cgaagacagg aaaaaaccat tgggagcggt tgaattttga tttgcaagat cagattgcgc
      361 tggatgattt cctattcaaa gtcgaattga agttaggaga aacacctgag atagcgaaga
      421 tgatagaaga gctcaggagc agccaaagat cgcatcgcca gattctttcg gatttagttg
      481 caatcgcaga tccgcaggca actcaaacct aaacggttgc cgacggctag cttttgtgta
      541 ccccgccgga actatagcgt ttcgccattt agtgttcata ctgaaacgat cgtttattgc
      601 ttgagaaggc tgcatccaaa gcaagcccgg taacgattgc atgccaagcc tcatcttccg
      661 agaggccctt ctgttttatt gaacgccaca ggattatcac ctcttcctcg gtgacgcttt
      721 ctagaagatc ctgctcccgt tcttgctcaa tgctaggttt gggattgtat ctagttagcc
      781 gataactcat tggtatttga cccttccttg tagtccttca aatgacaagg aacactagca
      841 aagtaggtgc cagctatgcg agcaccgaaa tgcatcgaat catggtttag gttaggcttg
      901 cgagggtcac ttggtaggcg caaagttgtc acaattgcgc aattttaaga caagagctgt
      961 aggaaaagcc ttcccgcact taactgagaa cggccacacc tgagatctca accatgcaat
     1021 ccggattgta aaggcttttc acttccacca gcgttatagc agggaagtgc cggccaaaca
     1081 cttctttgta cactgatcca agcggctttt tgtttctcag gtaggcttgc aaatccgtga
     1141 tatagatggt gaggacagcg taattccgtg gctctccgcc ggctgcctcg attgcggttt
     1201 ttaggtttcg gatcacctgg gcgaactggg ctacgaaatc tcccttgcca acgacttgcc
     1261 cctgagagtc gaacggcgcc tgtccggcta cgtggacaac ggtaccccca gtaatcttgg
     1321 caacgtgagc gtaaccgatt ggtgactcca aaccggtcgg atccaataat tcaaatggca
     1381 ttaacttctc tcctcggtga gccgggtcgt aacggaactc cattgctcga taaagctctt
     1441 catctcccct ttaacatcgt ccgtagccgc aacataaggc agaatgatcc gtgtggcgcc
     1501 cgccgccgca taaggccgaa tgtgttcgac aatttcgtgc gcagaagtgg cggcgatcag
     1561 cggcaaatcc gcgaacattc gatcactcac caatttacgc gccgaatgaa agcctccgct
     1621 tttccacgca ttgcgcatcg cctcaacttc cgctgcaaac cccatgcgtc cgagaagatc
     1681 gcgatagtaa tcggctagcg cataataggt tagcgcatgg ctcaaagcat ctcgcgccgc
     1741 ggcagaagtt ttagcgatag aacaacgaat cttgcagatg atctgcattc cggctggatc
     1801 tttcccgacc tcttcggctc ctttgcggac ctcatcggcg attcgccgaa tttcagcggg
     1861 attggccata ttgatcaaaa ctccatcagc aatcctgccc gccaggcgtg tcatcttggg
     1921 accgaaggcg gccagataga tcggtatggc gcggccggat ggcttgaatg ccatcttaaa
     1981 attgtgtgcc gagaaaaatc ttccctcgaa gtcaagcttc tccccccgta ttgcggattg
     2041 gacaatttcg atgtattcgc gaacccggcc gagcggttga tccaatacct gcccgtgcca
     2101 cttcgcaata gtcggattgg atgaaccgat tcctaagaga aatcttccgc gagataactc
     2161 gtccaagcct accgcttgca gcgctaaggt cgccggcgtc cggccgagaa tgtggtagat
     2221 cgcggaacct attttaagcc gggtagttac agcggcgata gctgacagca ttaccgtggg
     2281 atctttgtta ttggcctctc caccccaggc acaatcgaac ccatttgcct cggcgaggcg
     2341 cgccagctcc ggaattgcgt ccatcggcag ctgggcgccg gagttaaact cgatatcgat
     2401 tttcatagtg ctgcaaagat cttggaagga gactaaacat agctggctgg caaattggtc
     2461 ttcagaatct gaacctgctc agtacgctcg acataacgaa tgaagcgata cttgttttcc
     2521 acttcgtcga gcggatatgg gctaacatca gtgcgctcgg ttgtcacctt tgcgccaata
     2581 aagctcagcg cattcatggt tattcccgcc gaaatcgctc taccaaattc gctgagggta
     2641 tttgcccaaa tggagttctt gattcccgcc gcgtgcgcca ctcctactaa gtccgtccca
     2701 aaggcagttg ctgttttctt gctaccggag gcttcataca cttcattgtc gaaaacaata
     2761 tggagtaagt tgcttgggtg catgcgagcg atcgtcggta atgaacaaag attcattaac
     2821 aaagagccgt ctccatctaa ggctatgatc ttgcgttgag gcaagcccaa cgccatgcca
     2881 agtgcgatgg acgaacacag accgagggtg cgaacccgaa agttgccgtc tccagggcgc
     2941 agggcattcc actccagagt catcgctccg gccgaactga cgactacagc gtccttcgcc
     3001 tccgcagcaa cgagtttcaa gcaatcaaat cttttcatat gtccttcgac tccgcttggc
     3061 gaaaacaact acgctacagc gccttgagcg cggcgcgacg ccccaacact tcctttgtaa
     3121 ggagcagtgc gattggcctt ttcgaatcct cggccaagat ctgggcccgc ttgatcagct
     3181 ccggcgcgtc ggcggtctcg cgcatgatgt aatatcgaat cccgagcgcc tggagcaccg
     3241 gttccgtcat actgcccgat gtgctgaagg ttcgatcacc aacatcgccc gcatagtaga
     3301 tcatcagcag cagcgggata tgatactgca agagcgtggt aacgatggca ttattactgt
     3361 tgaagaatcc accgttctgc ataatcgccg cacacttctt tccgacgagg taggcccctg
     3421 cgcagatacc aattccttcc tcctcgcggc acacgggtac atgtagaagg tctggatcct
     3481 catcgacagc ctgaagcagc tgcgcgagat tgacgtcggg cagagtggcg atgaaattga
     3541 tcccggcttg ttttatcccg ctaaggatct ttgtagcagc tgcttgttcc gcgctcatcg
     3601 acgaaaactc cccgccaatc gctattcgct taacatcaaa gactagcctt atcaagcgag
     3661 ttgctacggt ttggctttgc gtttaaacgg aatctgagta taagtgcggt atcaatcacg
     3721 agccagaaag gacaactaat gatcaagcta tatgatttca agtcgtcgcc taattgccaa
     3781 cgggtgcgag ttgttctggc agaaaaaaat ctgccctacg aaaccgttcc agttgacctg
     3841 cgagcgcatg cgcaaaaaac tcctgaattc ctcaaactta atccgtatgg gaaagtccct
     3901 gtcatcactg atggcgacac gg
//
LOCUS       DASUTG010000447         4832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1699183, whole genome shotgun sequence.
ACCESSION   DASUTG010000447 DASUTG010000000
VERSION     DASUTG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699183"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VF089_10235"
     CDS             complement(<1..157)
                     /locus_tag="VF089_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011190398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6174384.1"
                     /translation="MNAEKVKTTYREAMRAAIRDALLRDERVFLMGEDVGRYGGCFAV
                     SKGLLDEF"
     gene            complement(231..1226)
                     /gene="pdhA"
                     /locus_tag="VF089_10240"
     CDS             complement(231..1226)
                     /gene="pdhA"
                     /locus_tag="VF089_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX6174385.1"
                     /translation="MPRHAKQKSQAIDREHALHLLKEMLRIRRLEEKCVELYTAAKIR
                     GFVHLYIGEEAVAVGAMPALESEDNIVATYREHGHALARGMSANVIMAEMYGKVEGCS
                     RGRGGSMHLFDAATRFYGGNAIVGGGIPLAVGLALADKIAGRQQITACFFGEGAVAEG
                     EFHESLNLAALWKLPILFCCENNLYAMGTALSRSESEIDICLKAAAFEIPAWPVDGMD
                     VIAVEDATRRAAIAARAGGGPHFLEYRTYRFRAHSMYDPELYRDKQEVDEWKKRDPIP
                     NLIERLKQQSLWQEEEWSRMEEEVAREIQQSVEFAEAGHWEPVEELTRFVYSEAL"
     gene            complement(1485..3245)
                     /gene="acsA"
                     /locus_tag="VF089_10245"
     CDS             complement(1485..3245)
                     /gene="acsA"
                     /locus_tag="VF089_10245"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6174386.1"
                     /translation="MGLTAIKKYPDRFHVAPNLIDYESTCATFSWEAAERELERSPEH
                     RGLNIAYHCVDRHANSAQRDHLALRWLSKSGEAREVTYDGLRRLTNRFANVLRELGIG
                     KGARVFALAGRIPELYTAALGTLKNGSVFCPLFSAFGPEPIHQRLSIGEGNVLLTTEA
                     LYSRRKIAELRASLPHLKYVLVIGESRPTQLSATHDFHTLMKNASENFEIARTDPEDM
                     ALIHFTSGTTGKPKGAVHVHKALIAHYITGNYALDLHPDDVFWCTADPGWVTGTSYGI
                     ISPLSHGIASIVDEADFDAERWYRIIQEQKVTVWYTAPTAVRMMMKAGTDIIQKYDLS
                     SLRFISSVGEPLNPEAVVWGQEAFGLPIHDNWWQTETGGIMIANYQSMEIRPGSMGRP
                     LPGIEAAIVQRHKDASIEQLKAEQVGELALRPGWPSMFRAYLHEEERYRKCFAGGWYL
                     TGDLAKQDADGYFWFIGRADDVIKSAGHLIGPFEVESALMEHRAVAEAGVIGIPDPII
                     GEVVKAFVSLKKDFEPSEALRRELLGHARSRLGPAVAPREIDFRDNLPKTRSSKIMRR
                     LLKARELGLPEGDLSSLEND"
     gene            3596..4111
                     /locus_tag="VF089_10250"
     CDS             3596..4111
                     /locus_tag="VF089_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX6174387.1"
                     /translation="MKTGPAYRYKLVGVKKRRKSGYDVFDGTLQKTNVWLNDIMNELD
                     WQEHPYKAYLALRTVLHALRDRLTIEEAVQLGAQLPMLVRGFYYEGWTLKGKPGKERH
                     KEDFLAPIKEAFKEDFSSRPESIARAVFKTLARHTSPGEIEDVKHSLPKALIELWPSK
                     RVTPSPGARPR"
     gene            4412..>4832
                     /locus_tag="VF089_10255"
     CDS             4412..>4832
                     /locus_tag="VF089_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX6174388.1"
                     /translation="MVATADKRDYYEVLGVDRTATQDQIKQAYRELAMTSHPDRNPAP
                     DATDRFREIAEAYAVLCDPAKREQYDVAGHAGVSERWSTEDILRGFDFGDFFGGRFAD
                     VGGIFGDLFGGRSHRVPVKPHGADLHHELSLSVEEAAK"
BASE COUNT         1077 a         1364 c         1296 g         1095 t
ORIGIN      
        1 caaactcatc gagcaagcct ttgctcactg cgaagcagcc gccgtagcga ccgacatctt
       61 cgcccataag gaaaacccgc tcgtcgcgca acagcgcatc gcgaatcgcc gcgcgcatcg
      121 cttctctata cgtggttttt actttttccg cgttcatatc tcgtctcggt tcaaggttcc
      181 aagttcaagg ttggggcggg gacgttgaac cttgaacatt gaacttttat ttaaagcgcc
      241 tccgagtaaa caaagcgggt taactcttcg acgggttccc aatggcccgc ctcggcgaac
      301 tccacagact gctggatttc gcgtgcgacc tcctcctcca tgcggctcca ctcttcctcc
      361 tgccagaggc tttgttgttt caaccgctcg atcaaattcg gaatcggatc gcgcttcttc
      421 cactcgtcca cttcctgttt gtcgcggtaa agctcgggat cgtacatcga gtgagcgcga
      481 aagcggtatg tacggtattc aagaaaatgc ggcccgccgc ctgcgcgcgc ggcgatggca
      541 gcgcgccggg tggcgtcttc gacggcgatg acatccatgc catccaccgg ccaggcggga
      601 atttcgaaag ccgcggcctt gagacaaata tcgatctccg attccgatcg agagagcgcg
      661 gtgcccattg cgtaaagatt attctcgcag caaaacagga tcggcagttt ccagagcgcg
      721 gcaagattca gcgattcatg aaactcgcct tcggcaacgg ctccttcgcc gaagaaacag
      781 gcggtaattt gttgccgccc ggcgatttta tctgcaaggg ccaagcccac agccagcggg
      841 ataccgccgc cgacaattgc gttaccgccg taaaaccgcg tggcggcatc gaacaggtgc
      901 atggacccgc cgcgaccgcg gctgcaacct tcaactttac cgtacatttc cgccattatc
      961 acattggccg acatgccgcg cgccagtgca tggccgtgct ctcggtaggt tgcaacgata
     1021 ttatcttcag actcgagggc cggcatcgcg ccgacggcaa cggcctcctc gccgatatag
     1081 aggtggacaa acccgcgaat tttagcggcc gtgtagagct ccacacattt ttcctcgagg
     1141 cggcgaatgc gcagcatctc tttaagcaga tgcagcgcat gctcgcgatc gatcgcttgc
     1201 gatttttgtt ttgcatgtcg aggcattgag aaagtctccc atgtcaccac gtagcacacg
     1261 aagagcatga aggtgagagg agaatgagaa aactcctccc gcatcttcgt gtccttagtg
     1321 gtaagtcact ccctatccgg gttgatatcc tcgatgataa aatccaattt caccacgatg
     1381 agcacaaagt tcggagtttt gttctcctaa acccttcgtg tccttcgtgc ctccgtggtg
     1441 aaagtatcct ttcttctcga ttgcggccat gccgcgctgt gccatcagtc gttctccaaa
     1501 ctcgacaaat ctccctcggg caaccccagc tccctggcct tgagcagacg gcgcatgatc
     1561 ttgctgctgc gggtcttcgg caaattgtcg cgaaaatcga tttctctcgg cgccacggcg
     1621 ggacccagac gcgagcgcgc gtggccgagc agctctctgc gcagcgcttc gctgggttcg
     1681 aaatcctttt tcaaagagac gaaggctttg accacttcgc cgatgatcgg atcgggaata
     1741 ccgatgacgc cggcctcggc aacggcgcga tgttccatca acgcgctctc gacttcgaac
     1801 ggcccgatga gatggccggc ggatttaatc acatcgtcgg cacggccgat gaaccagaag
     1861 taaccatcgg catcttgctt ggcgagatcg ccagtcaaat accaaccgcc ggcaaaacat
     1921 ttacgatagc gttcttcttc gtgcagataa gcgcgaaaca tcgaaggcca gccgggccgg
     1981 agcgctaact cgccgacctg ttcggcctta agttgctcaa tgctggcgtc tttgtgccgc
     2041 tggacgatcg ccgcttcgat gcctggcaaa ggccggccca tcgaaccggg acggatttcc
     2101 atggattgat aattagcgat catgatgcca ccggtctcgg tctgccacca attgtcgtgg
     2161 atcggcaagc caaacgcctc ctgtccccac accacggctt cgggattgag cggctcgccg
     2221 acactcgaga taaaacgcag gctgctcaga tcatatttct gaatgatgtc cgtacccgcc
     2281 ttcatcatca tgcgcaccgc cgtcggcgcg gtgtaccaga ccgttacttt ttgttcttga
     2341 atgatgcggt accaacgctc ggcatcgaaa tcggcttcgt cgacgatact ggctatgcca
     2401 tgtgaaagag gcgaaatgat gccgtaggag gtgcccgtca cccagccggg atcggcggta
     2461 caccaaaaaa catcgtcagg atggaggtcc agggcgtagt tgccggttat gtaatgagca
     2521 atcagcgctt tatggacatg gaccgcgcct ttcggcttac cggtcgtacc ggaagtgaag
     2581 tgaatcagcg ccatgtcctc cgggtcggtg cgagcgattt cgaaattctc cgaagcattt
     2641 ttcatcagcg tgtgaaaatc atgagttgcg ctcaactgtg taggtcggga ttcgccgatg
     2701 accagaacgt atttcaggtg cggcagcgat gctcgcagtt cggcgatttt acgcctggaa
     2761 tagagcgctt cggtcgtgag gagcacattc ccttcgccaa ttgacaaacg ttgatgaatc
     2821 ggttcgggcc cgaacgcgga aaatagcggg cagaacacgc tgccattttt gagagtaccc
     2881 aacgccgccg tgtaaagttc gggaatgcga ccggccaatg caaaaacccg cgcccctttg
     2941 ccaataccga gttcacgcag cacgttggca aagcggttcg tgaggcggcg caatccgtca
     3001 taggtaactt cacgggcttc gcccgatttg ctcaaccaac gcaacgccaa atgatcccgt
     3061 tgcgccgaat tcgcatgacg atcgacgcag tggtaagcga tattgaggcc gcgatgctcc
     3121 ggcgatcttt ccagctctct ttccgccgcc tcccaagaaa acgttgcgca ggtgctctcg
     3181 tagtcgatca agttgggtgc aacgtgaaaa cggtcgggat atttttttat tgctgttaat
     3241 cccacggcgt cctttcgggt cgagacacat agctacggcg atctgttcgg ccgacgcgct
     3301 tctctgccga atctgctcaa tccaacgctg caaaaccgtc taaaccgcaa gaaacaagcc
     3361 acaataaatc cggaactttg cacggtcagg gcgcactcgc gaagcggttt tttccagtcc
     3421 tgcgaagaga tgcgccggtt tttttcagaa gtgaaaattg agttgggctg aattaccgct
     3481 ttaattcgga ttcaaccgaa tatttttgct tttcaaccat tccttgcggc actggaattg
     3541 ctcaattcat ttacctgaga tcgcctcttc ggaaaattgt tagggaggaa ttgttatgaa
     3601 aaccggtccg gcataccgct acaaattggt aggagtcaaa aaaagacgca agagcggcta
     3661 tgatgttttt gacggcacct tgcaaaagac taacgtctgg ttgaacgaca ttatgaacga
     3721 gctggattgg caggaacatc catacaaagc ctatctcgcc ctgcgcaccg tgcttcacgc
     3781 cctgcgcgac cgcttgacca tcgaagaagc cgtccaactc ggcgcgcagt tgccgatgct
     3841 cgtgcgtggc ttctactacg aaggttggac gctcaaaggg aaaccgggca aggaacgaca
     3901 caaagaagat tttctagcgc ctataaagga agcgtttaaa gaggatttca gctcgcgacc
     3961 cgagagcatc gcgcgcgcgg tgttcaaaac tctcgcgcgc cacactagtc ccggcgagat
     4021 cgaggacgtc aaacatagct tacccaaagc gttgattgaa ttgtggccgt cgaaacgcgt
     4081 tacgccgtcc ccgggtgccc ggccgcgctg atgcagtacc aagctgtgta ttatgaaatt
     4141 cttttcttgg cgtactccac gtctccgcgg tgaattatct tctgaagcga ggcccctcag
     4201 gctagtcact ggaatatttg cgcgagcagc gacaacattc aatcacacta ttgcggaaat
     4261 cacgaagttt acgaattcag tcattcaaat ccttcgtgtc tttgacgggc ttcgtggtga
     4321 aacttttatt tcacttgatt gcggttgtcc tccactggta cacctttaag ccggggttgc
     4381 tgcggtttca gagcgtaggg aaaagatggc catggtcgca acggcagata aacgcgacta
     4441 ctacgaggtt cttggtgtag accgcacggc gacccaagat caaatcaaac aagcctaccg
     4501 cgagttggcg atgacctcac atccggaccg caatccagcg ccggatgcca cggatcgatt
     4561 tcgagagatc gctgaggcgt atgctgtgct gtgcgatccg gccaaacgcg agcagtacga
     4621 tgtcgcgggt cacgccgggg tgagtgaacg ctggtccacc gaagatatct tacgcggttt
     4681 tgatttcggc gactttttcg gcggtcgctt tgccgatgtc ggcggcatct tcggcgatct
     4741 ttttggtggg cggtcgcatc gagttcccgt caagccgcac ggcgcggatc tacatcatga
     4801 actcagcctt agcgtggagg aagcggcaaa gg
//
LOCUS       DASUTG010000448         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1253543, whole genome shotgun sequence.
ACCESSION   DASUTG010000448 DASUTG010000000
VERSION     DASUTG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253543"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..709
                     /locus_tag="VF089_10260"
     CDS             203..709
                     /locus_tag="VF089_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002203032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX6174389.1"
                     /translation="MSKRNLIIGIASVALAIAWYAFRPELLFINKTVNEELPVAQAGS
                     VSMAKETEAMVLAKGDFRGLAHETKGEATVHRLADGKRILRLSDFETSNGPDVHVYLV
                     AAEVAKDNATVKQAGFIDLGSLKGNKGDQNYDVPADADLNKYKSVSIWCARFGVNFGA
                     ATLGSQSS"
     gene            785..2098
                     /locus_tag="VF089_10265"
     CDS             785..2098
                     /locus_tag="VF089_10265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6174390.1"
                     /translation="MARGRKQIVILGGGFGGLYTARELEKKLGADPDVEITLVNRENF
                     FLFTPMLHEVAASDLDPADILNPIRKLLKQSRFFVGNVEAIDLDSKKVKVSHGEGQHP
                     HELAYDQLVIALGSVSNFFNLPGVEERALTMKSLNDAVTLRSRLIEQLDEADFECCPA
                     MRESLMTIVVAGGGFAGVETVAGVNDFLREAVKYYPNLSEEMIRVVLVEHGPAVLPEL
                     DGSVGVYAAEKLSRRGVEIRLQNAVTSMSERAVTLSDGRSINTRTLIWTAGTAPNPLL
                     AALACSKEKGKLIVDENLEIPGRPGVWALGDCAAIRDHQSGKLYPPTAQHALRQGKVV
                     ARNVIAAVRGTAKRPFVFSTIGQLAAIGRRTGVAHILGVNFSGYIAWWMWRTIYLSKL
                     PRLEKKVRVVLGWSLDLFFSKDLVQLKTNDATVCRGGAVKQQSAA"
     gene            2114..>3653
                     /locus_tag="VF089_10270"
     CDS             2114..>3653
                     /locus_tag="VF089_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6174391.1"
                     /translation="MSSQDHYDVVIIGTGAGGGTLAHKLAPSGKRILLLERGGYVPRE
                     KDNWNPRAVNLEGKYQTKEIWHDRDGHELHPHTNYYVGGNTKFYGAALFRLRKEDFGE
                     IRHHGGLSPAWPIAYDDMEPYYTEAEQLYHVHGNRGEDPAEPPASAPYPHPAVSHESR
                     IQQLSDDFAQLGLRPFHTPLGVMLDEKDSHASRCIRCNSCDGFPCLVDAKSDAQVCAV
                     DPALEHSNVTLRTGAYVERLQTSPSGREISKVMIKSHGVREEISTDIVVSSCGAINSA
                     ALLLRSANERHPNGLANSSDVVGRHYMGHVNSVLMALSKCPNPTIFQKSLSVNDFYFG
                     TAGWNYPMGHISFVGKLDGETLKGGAPALTPGWTLDMMARHSLDFWLTSEDLPDPNNR
                     VTLDSAGGIVLSYKANNEEGHKRLIVKLKELMQQQTACKIHGRECHQGLFGRNLYVGQ
                     RIPLAGVAHQVGTVRFGNDPRTSALDANCKAHELDNFYVVDGSFFCSSGAVNPALTIM
                     ANALR"
BASE COUNT          807 a         1033 c         1043 g          770 t
ORIGIN      
        1 cggtaaagat actcggcctg atccttttgc attcccgcta aagaggttga gcttcagcga
       61 accaagctgt tctcaccgag agagtatccc gctctaaaca cgattctggt tttgcgaagt
      121 ctgcatcgtt tcactccttc ccgacatcta accaacagga tttccccagt catagtcata
      181 aacaattaac ggaggataag tcatgagtaa acgtaatctt attatcggga tcgctagcgt
      241 cgcgctggca atcgcttggt atgcgttccg gcccgagttg ctcttcatca acaaaaccgt
      301 gaacgaggag ctgccggttg ctcaggcagg ctcagtatcg atggccaaag aaacagaggc
      361 catggttttg gcgaagggcg attttcgcgg gcttgcgcac gagaccaaag gcgaagcgac
      421 ggttcatcga ttggcggacg gcaagcgcat actgcggctg agcgattttg aaacttccaa
      481 tggtccggat gtccatgtct atctggtcgc cgccgaagtg gccaaagaca atgcgactgt
      541 caagcaagcc ggcttcatcg atttgggttc actcaaaggc aacaagggcg atcagaacta
      601 tgatgtgccg gcggatgccg atctcaacaa gtacaagtcg gtgagcatct ggtgcgcgcg
      661 tttcggggtt aactttggcg ccgctactct tggttcacag agcagctaga ggaccgctat
      721 tccttgtcgg ctgctttccc tgcgcgggaa gcagccggtt tgagaaagta cgaaaggatt
      781 cgagatggcc aggggcagaa aacagattgt gatcctcggc ggcggcttcg gcggtctcta
      841 caccgcccga gaactggaaa aaaagctcgg cgcggatccg gatgtcgaga taacgctggt
      901 gaaccgcgaa aatttctttc tctttactcc gatgttgcat gaagtagccg ccagcgacct
      961 cgaccctgcc gacatcctga atccgatccg caagcttttg aagcaatctc gtttcttcgt
     1021 cggcaatgtc gaggcgatcg acctcgactc caaaaaagtc aaagtctctc atggagaggg
     1081 ccagcatccg catgagctgg cgtacgatca actggtgatc gcgctgggct cggtcagcaa
     1141 tttcttcaat ctccctggag ttgaagagcg cgcgctgacc atgaagtcac tcaacgatgc
     1201 ggtgaccttg cgcagccggc ttatcgagca gttggacgaa gccgattttg aatgttgtcc
     1261 ggcgatgcgt gaatcgttga tgaccatcgt tgtagccggc ggcggttttg ccggcgtcga
     1321 gacggttgcg ggagtaaatg atttcttacg ggaagccgtg aagtattacc ccaacctcag
     1381 cgaagaaatg atccgcgtcg tgctggttga acacgggcct gcggtcttgc cggagctcga
     1441 cggcagcgtg ggtgtttatg ctgcggagaa gctttcccgg cgcggagttg agatacggct
     1501 ccaaaacgcc gtgacttcca tgtcggagcg cgccgtaacg ctgagcgacg gcaggtctat
     1561 caacacgcga actctcatct ggacggcggg caccgcgccg aatcctttgc tggcggcgct
     1621 tgcctgctca aaggagaagg gcaagctgat cgtcgacgag aatcttgaaa ttcccggccg
     1681 gccgggcgtc tgggcgctgg gcgactgcgc cgcaattcgc gatcaccagt ccggcaagct
     1741 ttatccgccg acagcgcagc acgcgttgcg tcagggaaaa gttgtcgcgc gcaacgtcat
     1801 cgccgcagtt cgaggaacgg caaaacgacc gtttgttttt tccaccatcg gccaactcgc
     1861 cgccatcggc aggcgaaccg gagtggcgca catcttgggt gtcaacttct ccggctacat
     1921 cgcgtggtgg atgtggcgaa ctatttacct gagcaagctg ccgcgcctgg agaaaaaagt
     1981 acgagtggtt ctgggctggt ctcttgatct gtttttttcc aaggatctcg tacagctcaa
     2041 aaccaacgat gcgacggttt gccgcggcgg tgcagttaaa caacaatccg ctgcttgagt
     2101 aaggaatagg aacatgtcca gccaagacca ttacgatgtt gtcattatcg gcactggcgc
     2161 aggcggcggc accttggcgc ataaactcgc gcccagcggc aagcgcatcc tcctcctgga
     2221 acggggcggg tatgtgccgc gcgagaaaga caactggaat ccccgcgccg tcaacctcga
     2281 aggaaagtat cagaccaaag agatttggca tgatagggac ggccacgaac ttcatccgca
     2341 caccaactac tacgtcggcg gcaataccaa attctacggc gcggcgctgt tccgtctgcg
     2401 caaagaagat tttggtgaaa tccgtcatca cggcggcctc tctccggcat ggccgattgc
     2461 ttacgatgac atggagccct actacaccga ggcggaacag ctttatcacg tgcacggcaa
     2521 ccgcggcgaa gatccggccg agccgccggc gagcgcgcct tatccgcatc cggcggtcag
     2581 ccatgaatcg cgcattcaac aattatccga tgatttcgcg cagctcggcc ttcgcccatt
     2641 tcacacgcca cttggcgtga tgctcgacga aaaggattcg catgcgagcc gctgcattcg
     2701 ctgcaatagc tgtgatggat tcccttgtct ggttgacgcc aaatccgacg cgcaggtctg
     2761 cgccgtcgat ccggcgctcg aacatagcaa cgtcacgctc agaaccgggg cttacgtcga
     2821 acggctccaa accagcccat cgggccgtga gatcagcaag gtaatgatca agagccatgg
     2881 cgttcgcgaa gaaatttcga cagatatcgt ggtctcgtct tgcggcgcga tcaactcggc
     2941 ggctttgctg ctccgctctg ccaatgagcg gcatccgaac ggcctggcca atagttccga
     3001 tgttgtcggc cgacattata tgggccacgt caactcggtc ctgatggctc tatcgaaatg
     3061 ccccaacccg acgatctttc agaaatcgct ttcggtaaac gatttctatt tcggcacggc
     3121 cggctggaat tatccgatgg gccatatctc gttcgtcggc aagctcgacg gggagacatt
     3181 gaagggtggc gcgccggcgc tcacgcccgg ctggactctc gacatgatgg cgcgccattc
     3241 gctcgatttc tggctcacgt cggaggatct gcccgatccg aataaccggg ttacgctcga
     3301 ttccgccggc ggtatcgttc tcagttacaa agccaacaac gaagaaggcc acaagcggct
     3361 catcgtcaag ctgaaagaat tgatgcaaca gcagaccgcg tgcaagatcc acggccgcga
     3421 atgccatcag ggactgttcg gccgcaacct ctacgtcggt cagcgcattc cgctggctgg
     3481 tgtggcgcat caggtcggaa cggttcgttt cggcaacgat cccagaacca gcgccctgga
     3541 cgccaattgc aaagcgcacg aactggacaa tttctacgtc gtcgatggca gctttttctg
     3601 ctcgagcggc gcggtcaatc cggctctcac catcatggcc aacgcactga gag
//
LOCUS       DASUTG010000449         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1550595, whole genome shotgun sequence.
ACCESSION   DASUTG010000449 DASUTG010000000
VERSION     DASUTG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550595"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..525
                     /locus_tag="VF089_10275"
     CDS             82..525
                     /locus_tag="VF089_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174392.1"
                     /translation="MAELKVDGVVHWSIPVNNLEEAEKFYGELLGLEPKGRLGGSGMS
                     CFKAGDHNILLCVRNDATVRTPQQDNRLHHAFMVSPEMWERGCRVFHENGVKIAEPIV
                     YREKGHFTGRELYFLDPSGNMLELCDPTWKPGMPAPTYEEIVGAN"
     gene            complement(612..1874)
                     /locus_tag="VF089_10280"
     CDS             complement(612..1874)
                     /locus_tag="VF089_10280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174393.1"
                     /translation="MTTDSETNGANDPPAPIRPWSSLLIRDYRLIFIAILCANTSMHM
                     RNVASLYQVYQLSGSSVQLGLAGFFQAVPFIFFGLFGGVLADTYNRKKLILFTAAFNL
                     LPGLALGLLTISGVVQVWHINTLNVVTSSLQVLGGPARQAIIPSLVPQSYLLNAVTLS
                     TLMMQGTQLTAPVFAGFLIDFFGLDASYIADALLLVPAICCVLLVRSSGQPQGARRQV
                     SWRSLIEGFEFLYHTRIILSLFLLDFFAVLFGFYRPILPIFADDVYQVGARGLGALYA
                     APAIGALIGSGMLLALGDIKRKGALSIAATLLFALSLGFLGLSKWFWLGLIAVGVLGL
                     ADAISVTMRRTVVQILAPDDMRGRATSFLTVFAQTTNALGAVIAGAGAALLGAPNATL
                     VGCILCTLTVLAVCWAIPQLWWYRSDAP"
     gene            2027..3049
                     /locus_tag="VF089_10285"
     CDS             2027..3049
                     /locus_tag="VF089_10285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174394.1"
                     /translation="MKKLITSTAMIVCIFFFTGAASAQNNFYEGKTIRIIVGFSPGGG
                     YDALARMLSRHMPKYIPGNPTIVVENMTGAGSLLAANHIFNVAKPDGLTFGHFSGGFA
                     FNQVMGQPGIEFDARKFVFVGAVARDESAIALTKASGISNMEQWMAAKTPVKMGTTGP
                     GAFGTDNVIKVIKAALNLPIQIISGYKGTAEMRLAAESGEIDGTTWGWDTMRGAWQKA
                     IESGTVLIVLQTVPKPFPDLPRVPLAIDLAKTPEAKQLIEVGIHYPSKITKTLALPPG
                     TPNDRAQILQKALQDTVKDKEFIAEAEKAKLGLAPVTAEEMKTTVDGIFKLDPALMGK
                     LKTILF"
     gene            3142..4287
                     /locus_tag="VF089_10290"
     CDS             3142..4287
                     /locus_tag="VF089_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174395.1"
                     /translation="MRVIDSDGHFHEPHYLFEEYIEKEYWGKRPRVVKIQGHSMEEGR
                     WAVEGKVVPRIPLSKGVGAGGFLYMSPRHKQMHMKDSSLDDVDGRVKDLSLLGVDIQV
                     IYPTAFAFISDVEDKGLAAAICRAHNNYVAERCKQAPDKLKGIALVPIQDPPAAVEEM
                     RRAVKNLGLVAVTIPGMVGNRPLHSPEFYTFFKAANDLDCPIGFHAVTGMHYTPWADC
                     FTDFFSTHITCMPFSMMVALMSLTQMGVMAEFPKLRCAFLEIDGTWMHYWAGWVNKHV
                     SDAKRVSAGHHLEPGWGESPYLLPATTRDPMEYIKSGRFLSGYDEHEDLRYLLDRLGA
                     KTFMYASDYPHSDTEWERVATTKALETISAEEKNAILGNNAARFYGI"
BASE COUNT         1074 a         1127 c         1248 g          926 t
ORIGIN      
        1 ttgctcgaga aaagttaacc atacttgcaa aatctcagtt cgttgtgatt ctatcgctca
       61 attgattgtc gggaggtgct tatggctgaa ttaaaagttg acggtgtggt ccactggtcg
      121 attccggtaa acaatctgga ggaagccgag aaattttatg gcgagcttct tgggttggaa
      181 cccaagggac gtttgggcgg gtcggggatg tcctgcttta aagccggcga tcacaacatt
      241 cttctctgcg tccgcaacga cgcaaccgtc cgaactccgc aacaagacaa ccggcttcat
      301 cacgcattta tggtgagtcc ggaaatgtgg gaaagaggat gccgggtgtt tcacgaaaat
      361 ggcgtgaaga tcgccgagcc gatcgtgtac cgggagaaag gtcatttcac cggtcgcgaa
      421 ttatacttcc tcgatccgag cggcaatatg ttggagctgt gtgatcccac ttggaagcct
      481 ggtatgccgg cgccgaccta tgaggaaata gtcggcgcga actaaatatc tgaagtttct
      541 acccggccga ggagaaacgt attttgttca aaactggtca agttcaactt tgaacgtttg
      601 aaccctcttc ctcaaggcgc gtccgagcga taccaccaca gttgcggaat cgcccagcaa
      661 acagccagaa ccgttaacgt gcaaagtatg cagcccacca gcgtggcgtt gggcgcgcca
      721 agcagcgccg ctccggcgcc ggcaatgacc gctccaaggg cgttggtagt ttgggcaaag
      781 accgtcaaaa aacttgtggc gcggccgcgc atatcgtcgg gtgcgagtat ctgcaccaca
      841 gtacggcgca tggttacgct gatggcgtca gctaatccca aaacaccgac cgctatcaac
      901 ccaagccaaa accatttcga caaccccaaa aaccccaagc ttaaagcaaa gagcaatgtc
      961 gcggcaatcg acagcgctcc cttgcgcttg atgtcgccga gagcaagcaa catgccggag
     1021 ccgataagag ctccgattgc cggcgcggcg tagagcgcgc cgagtcctcg ggcgccgact
     1081 tggtagacgt catcggcgaa tatcggcagt atcggacgat aaaaaccaaa cagcacggcg
     1141 aagaagtcca agaggaatag ggacagaatg atacgcgtat ggtagaggaa ctcgaatccc
     1201 tcaatcagac tgcgccagct tacctgtcgc cgcgcccctt gcggctgccc tgaactgcgt
     1261 accagcaaaa cacagcagat cgccggcacc agcaataatg catcggcgat atacgaagca
     1321 tccaagccga agaaatcgat caggaacccg gcaaagacgg gcgcggttag ctgcgtccct
     1381 tgcatcatca aggtcgacag agtcactgcg tttagaagat aggattgcgg cactaagcta
     1441 gggataatcg cctggcgcgc cggaccgccg agcacctgta gcgacgaggt gacgacgttg
     1501 agagtattga tgtgccaaac ctgaaccacg ccgctgatcg tcaacagccc tagcgccaag
     1561 ccgggcagca aattgaatgc ggcggtgaag aggatcaatt ttttacgatt gtatgtgtcg
     1621 gcaagcacgc cgccgaacag cccaaaaaaa ataaacggca ccgcctgaaa aaatcccgcc
     1681 aaaccaagct gcaccgacga gcctgataat tgataaacct gatataaact tgcaacgttg
     1741 cgcatgtgca tcgaggtgtt ggcacacagg attgcgatga agatcaggcg atagtctcga
     1801 atcaggagag acgaccaggg tcggatgggt gccggggggt cgttggcgcc gtttgtttct
     1861 gaatctgttg tcactgggaa tgggagacga gtgatggaat gtgagtggca atgtagctgt
     1921 tgcccgagca ggcgtcaact ttatcaatga ttaaaagttt tccgcttgac gttttaccgg
     1981 catctccatt agctttgcac gtcaagattg tagggagggc cggttaatga agaagctgat
     2041 aacgagcaca gcaatgattg tctgcatatt cttttttacc ggggctgcat cggcccagaa
     2101 taacttctat gaggggaaaa ccatccgcat cattgtcggg ttctcgcccg gcggcggtta
     2161 cgatgctctg gcgcggatgc tttcacggca catgcccaag tatattcccg gcaatccgac
     2221 cattgtcgtg gaaaacatga ccggcgctgg aagcctgctc gccgccaatc acattttcaa
     2281 tgttgccaaa cccgatggtc taacgttcgg tcacttcagc ggcggttttg ccttcaatca
     2341 ggtcatgggg cagccgggta tcgaattcga cgcgcgcaag tttgtttttg tcggagccgt
     2401 ggcgcgcgat gaaagcgcta tcgctttaac caaagccagc ggcatcagca atatggaaca
     2461 gtggatggcg gccaagacgc cggtgaagat ggggaccaca gggccgggcg cgttcggcac
     2521 cgataatgtg atcaaagtca tcaaggccgc gttgaacctg ccgatccaga tcatctccgg
     2581 atacaagggc acggcggaga tgcggctcgc tgccgaaagc ggtgagatcg atggcacgac
     2641 gtggggttgg gataccatgc gaggggcttg gcagaaggcc atcgaatcag gcacggtgct
     2701 gatcgtgctg cagaccgtac ccaaaccatt tccggatcta ccgcgcgtgc cattggcgat
     2761 cgatctggct aaaacgccgg aggcaaaaca actgattgaa gtcggcatcc attatccgag
     2821 caagatcacc aaaactctcg cactgcctcc gggtacgccc aatgaccgcg cgcagattct
     2881 gcaaaaagcc ttgcaggaca cggttaaaga caaggagttt atcgccgagg ccgagaaagc
     2941 gaagctcggt ctggcgccgg tgacggccga ggagatgaaa actacggtgg acggaatctt
     3001 caagctggac cccgctctca tgggaaagct gaagaccatt cttttttaag ttccgtcgcc
     3061 gcagtctacc gaccggatgg ttgaaatcgc ccgccatagt cccgtcgatt aaattaactt
     3121 ggcagaatgg gagaagcact tatgcgcgtt atcgattcgg atggtcattt ccatgagccg
     3181 cattacttgt tcgaggaata catcgagaag gaatattggg ggaaacgacc acgcgtcgtg
     3241 aaaatccaag gtcattcgat ggaagagggg cgttgggcgg tggagggaaa ggtcgtgccg
     3301 cgaattccgc tttccaaagg cgtcggcgcc ggcggctttt tgtacatgtc gccgcggcac
     3361 aaacagatgc acatgaagga cagctcgctc gacgatgtcg acggccgtgt caaagatcta
     3421 tcgctcctgg gtgtcgatat tcaggtaatt tatccgacgg ctttcgcgtt tatctcggac
     3481 gtggaggata aaggcttggc cgcggcgatt tgtcgcgctc ataacaacta tgtcgccgag
     3541 cggtgcaagc aagcgcccga caagctcaag ggcattgcgc tggtgccgat tcaagatccc
     3601 cccgccgcgg tcgaggagat gcgtcgggcg gttaagaatt taggtctggt ggccgtgacc
     3661 attcccggca tggttggcaa ccgcccactt cattcgcccg agttctatac gttcttcaag
     3721 gctgccaatg atctggattg tcctatcgga tttcacgcgg tgacggggat gcactatacg
     3781 ccgtgggcgg attgcttcac cgatttcttt tcgacacaca tcacctgtat gcccttctcc
     3841 atgatggtgg ctctcatgtc cttgacgcag atgggagtta tggccgagtt tccaaaactt
     3901 cgctgcgcct tcctggagat tgacggcacc tggatgcact actgggcagg atgggtgaac
     3961 aagcacgtct cggatgcaaa acgtgttagc gctggacatc acctggagcc aggctggggc
     4021 gaatcgccgt atcttcttcc ggcaaccacg agagatccaa tggaatacat caagtccggc
     4081 cgctttctca gcggctatga tgaacatgaa gacttgaggt acctgctcga tcgtttaggc
     4141 gccaagactt tcatgtatgc cagtgattat ccgcacagcg ataccgagtg ggaacgtgtt
     4201 gcgaccacca aagcgctgga aacaataagc gctgaggaga agaacgccat tttaggcaac
     4261 aatgcggcgc gattttatgg aatatgaatt tatgttccag tttcacggct tgtcgccgat
     4321 caggaggaga aaatgagaaa gagagtcagt ggcgccctct tgatttctat ttgct
//
LOCUS       DASUTG010000450         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1403538, whole genome shotgun sequence.
ACCESSION   DASUTG010000450 DASUTG010000000
VERSION     DASUTG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403538"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VF089_10295"
     CDS             complement(<1..595)
                     /locus_tag="VF089_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX6174396.1"
                     /translation="MNRSQTFQPITASLWSARNAAQIFDGKIFGLMIALTMGWLVMQL
                     PTPALLDASGLYFLGSLTAGTLLLMFNVYDEYLVGILMLLFWVVSDSVPSEVALAGFS
                     RTSWFFVVAVLGNAVADKKSGLFHSIAVGTLRRIPPSYRMYTWLLTISGLIVTPVMPT
                     SKARMAIAAPLSRSISETLGFERRSIGSAGIGLSAYVG"
     gene            complement(702..1109)
                     /locus_tag="VF089_10300"
     CDS             complement(702..1109)
                     /locus_tag="VF089_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174397.1"
                     /translation="MIGDKREPAHLSIHVILELGGFLSLSRLPSSKKTDDAFVQRQSL
                     LAFIVTPMMPPFTGNGLKLRSVNGIGFALSVLVGSSRLIRNKMQAIGERFYVARVSSA
                     ELEDIAVCEGKGLVTEEMYGLNSYSSLRKSETI"
     gene            2155..2517
                     /locus_tag="VF089_10305"
     CDS             2155..2517
                     /locus_tag="VF089_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6174398.1"
                     /translation="MKTSKPTLGPRSSLFVIGLLVLIGLNGCSGGSLTTREKGAGIGA
                     VGGAGAGALIGNAVGRPGAGAAIGGVLGLGAGALVGDQLQGHENTNQQQQQQIQANQA
                     EVERQRLELQRMKQQREY"
     gene            complement(2717..2793)
                     /locus_tag="VF089_10310"
     tRNA            complement(2717..2793)
                     /locus_tag="VF089_10310"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2757..2759),aa:Val,seq:cac)
     gene            complement(3045..3713)
                     /gene="pyrE"
                     /locus_tag="VF089_10315"
     CDS             complement(3045..3713)
                     /gene="pyrE"
                     /locus_tag="VF089_10315"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX6174399.1"
                     /translation="MSEVSKKVAEMLLSTESIQVYKEAPFVFVSGRISPVYIDCRKLL
                     SFPTEREYIVAALARLVEAEIGFDKIDVVAGGETAGIPYASFVAHIIKKPMIYIRKQP
                     KGYGGTKQIEGILDPGKKVLLVEDLVTDGLSKLRFNIGIRGAGAKMTHCLCVFDYASD
                     RLNQHEGKDNLAKNDISLHVLANWDDVLDTGLAKSYFTESASKHITDFLQDPDNWGRK
                     MGFV"
     gene            3823..5127
                     /locus_tag="VF089_10320"
     CDS             3823..5127
                     /locus_tag="VF089_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX6174400.1"
                     /translation="MGMKPHTRDAILRNRPDITINKRRLLRDLNAISRIGLGDHGSVT
                     RLVFSIKELRSRQFLIHQMRQIGLQINIDRIGNIFGKLEGSDPKAPAVVAGSHLDTVI
                     QGGKFDGTMGVIAALEAARTLKENNVPLRSPVEVVCFVGEESSRFGFSTLGSSLVAGE
                     VRPEDFVNASDLQGAKLEDILAGLGIHRNNLLSLRRDPSTLKAYLELHIEQGPILEAR
                     KKPIGVVTSIAAPTRFKVIITGQADHSGTTPMEMRKDALVSAAELIVAIEGICREHSE
                     MAKGRVVGTVGALKIEPGVINAVPGKAELAVDVRSITASAKNRVVRLIEAKIREVASR
                     RKIRIKILPIRKEDPVPLNKRVIHLLQDCCEAREVPYEIMPSGAGHDAMQMAKITSAG
                     MLFIPSRRGISHNPLEWSAPEDISLGAQLLLDAIVRAANEEI"
     gene            5114..5653
                     /gene="pyrR"
                     /locus_tag="VF089_10325"
     CDS             5114..5653
                     /gene="pyrR"
                     /locus_tag="VF089_10325"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX6174401.1"
                     /translation="MKKSKNVRLLTAPEIKKNLERMGREILRQNKASDSLAFIGIRTR
                     GLFLAQRVRAIIQKFQHKEIPLGEMDISLYRDDLNALLPEGKVDHTSIPFDINNKTII
                     LFDDVLHTGRTVRAAVDHLIDLGRPKAIHLAVLIDRGGREFPIAANFIARKITLPQGG
                     EVRLKLKEVDGRDEVVVGR"
     gene            complement(5779..>6078)
                     /locus_tag="VF089_10330"
     CDS             complement(5779..>6078)
                     /locus_tag="VF089_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174402.1"
                     /translation="EFEDRKIDLFLQLFRDAVTGVEPESIEVAGFCSEDDTVTFGLLP
                     TEIIESLMTKCTSYFQPRELEDLRRFIDSHREPCDVLALVMRRHLKVEQSASCTH"
BASE COUNT         1569 a         1531 c         1559 g         1419 t
ORIGIN      
        1 aaccgacata cgctgagagg cctattccag ccgagccgat agagcgcctt tcaaacccca
       61 atgtttccga gatcgatcta ctgagaggtg cggctatggc catacgagcc ttactcgttg
      121 gcatcaccgg ggtgactatg agaccagata tggtgagtaa ccaggtgtac atccggtaac
      181 taggcggtat ccggcgcaga gttcctacag ctatactatg aaacagccca gattttttgt
      241 cggctacagc gtttccaaga actgcaacga caaagaacca tgacgtcctg gagaacccgg
      301 ccagcgctac ttccgagggc acggaatccg aaactaccca aaaaagcaac atcaatatgc
      361 cgacaaggta ctcgtcgtat acgttgaaca tgaggagcag cgttccggcg gtaagactac
      421 cgagaaaata gagtccgctc gcatccagga gggcgggagt cggaagctgc atgactagcc
      481 aacccattgt caaagcgatc atcaggccaa aaatttttcc atcaaatatc tgcgccgcgt
      541 tccttgctga ccacaacgac gccgttatcg gctggaaagt ctgactgcga ttcatcgaac
      601 cacctcagat gctaagccgc gctccgaagg tgcttcttcg gcatttgaca ggggttcgaa
      661 gagaatttta gtcatcgctt caataacaaa ttctccgccc atcatatcgt ttccgacttt
      721 cttaaacttg agtagctatt caggccatac atctcttcag tgaccaaacc ctttccttca
      781 caaacagcta tgtcttcaag ttcggcggat gacacccgcg caacgtagaa gcgttctcct
      841 atagcttgca tcttgtttcg gatcaggcgg gaactgccaa ccagaacaga taaggcaaag
      901 ccgatgccgt ttactgatcg gagttttagc ccgtttccgg tgaacggtgg catcattggg
      961 gtgacgataa atgccaagag tgactgtcgt tgaacaaaag cgtcgtcagt ttttttacta
     1021 ctcggcaatc ttgagaggct gagaaaccca ccgagttcaa gaatcacgtg gatggagagg
     1081 tgggccggtt ctcgcttatc gccgatcatt tcaagctgag caccaatctg caggaacggc
     1141 gtaagcgttg ctcgctatga cactgcagtt acgccacgat tactgtgccg tggaattcag
     1201 gatcgctgaa acaccgcacg cttcgcaagc agctctctgg cagaactcgg ttggctgatg
     1261 tctcaccgaa attgaaatca tgcgtcataa ccctgagaaa ctggagtttg aggaaagctc
     1321 atttgcgcag ttttttgccg gctggcattg atgttgtttg cgaatccgat gacggcgctg
     1381 ctgagatgcg cgtctgttga agcgcaacgg caaagtagct gtgatgtttc cgtcgacagt
     1441 cgacaaaatc catttcatgg actgcctctt gagattgtcg gcgcatcggc aacatccctg
     1501 cgcccggcgc atagtcaggt atgttcgcac ccggtgatat cggactccta cagacatgta
     1561 ccagaaggtc ggttttcacg atctcgcgat tccatcctgc ggatcttcgt agacgttatc
     1621 cctctgatgc ggcagggagg tttaattgga ctcaatttca ttcgctcacg gaagcgcaag
     1681 gagacgcgat gaagaagtca ggcgaaattg taaagagatc cgtgacgcaa cgagtgaaaa
     1741 cttcgtgggg agaagtggtc cgtttttcca tcacgatcca agccagccga tagccgcact
     1801 caagacaatg aggtgaggca ctagccatcg tcatcttcac cccataacac tttccccaac
     1861 cctcatttgt taataatggt atcaatccca gcgcaacgac ggggtccggc caccaccatt
     1921 ctaagatgta attagcgcca agaccgatca gaacagttga atctgtagat cacaaacgag
     1981 agtttccttg gctccgccct taacgacgtg aggctctatg accgggagtg ggcgcaggtc
     2041 gttacgacga tgctgcgaga gctgtggtgc atttattgca cgaaccagaa acctagataa
     2101 aagattctac ctcgtctatc gccgaggctg taaaaaggag ccgtagtaag aagtatgaag
     2161 acatctaagc cgactctagg tcccagatca tcgctgttcg taattggact actcgtgttg
     2221 attgggctaa acggttgttc tggcggatcg ctgaccacgc gggaaaaagg tgcggggatt
     2281 ggagccgtgg gaggtgccgg tgctggagcg cttattggaa atgccgttgg acgtccgggt
     2341 gctggcgctg ctatcggcgg agtcctgggg ctaggtgccg gcgcgttagt cggcgatcaa
     2401 ctccaaggtc atgaaaatac caatcagcag caacagcaac agatccaggc taaccaggct
     2461 gaagtcgagc gtcaacgact agagctgcag cgcatgaaac aacaacgaga gtactaacga
     2521 ctaggctgct aagccgactc gcgatggtta gtgtgtcaaa tctcgcttca ctcttgctgg
     2581 atttcccatg cacgtctatc tatcacgacc gatgtcacta ttatggatat caatagtagc
     2641 ggttatgatt acagaactgt ctctgtctgc tacaccggaa aaagcgaaaa ggcaaagttc
     2701 tttagactgt tggaaatggt ggtcgcagag ggatttgaac ccccgacctc tcgcatgtga
     2761 ggcgagcgct ctaaccaact gagctatgcg accataggat ggtttatcta acaccttgaa
     2821 caatgagctt caacaatcgc aaagccagtt cagcaatcaa agaacaaacc gctcgatata
     2881 tctcttacat acagaggata aactgcggta gtcgtagccg attctgaaac agcgactccg
     2941 atcgaatacc gccatgctgt gaatgcccga ttgattccgg gcatttggaa ctcacttccc
     3001 tggcgataac tcgtccgctt tgaacgacgt tgctagaaat agatttacac gaaacccatt
     3061 tttcgacccc aattatcagg gtcctgtaag aaatccgtga tgtgcttact ggcgctttca
     3121 gtgaaatagc ttttggccaa tccggtatcc agcacgtcat cccagtttgc gaggacgtgc
     3181 agcgaaatat catttttcgc taaattgtcc tttccctcat gctggttaag ccggtcggaa
     3241 gcataatcga atacacacaa acagtgggtc atctttgctc cagcgcctcg tattccgata
     3301 ttgaaacgaa gcttgctcaa tccgtcggtg accaggtctt ctaccagcaa cactttcttt
     3361 cccggatcga ggatgccttc tatctgttta gtcccgccgt accctttcgg ctgcttgcga
     3421 atatagatca tgggtttttt gataatatgg gcaacaaaag aagcgtaggg aatgcccgcc
     3481 gtctcgcctc cggcgacgac atcgattttg tcgaatccta tttctgcttc aaccaatcta
     3541 gctaatgccg cgacgatgta ttcacgttcc gtcgggaagg ataggagctt gcgacaatca
     3601 atatacaccg gcgagatgcg tccggatacg aagacaaagg gagcttcctt gtagacttga
     3661 atggactcag tcgagagcag catctcggca actttcttcg aaacttcgct cataagccga
     3721 cctttcaaaa ttggatatgc agctatagca cacgaattcg gctcttccaa cttggtctaa
     3781 ccatcgtgta gtttttcagt atcggaggaa gctgctaaga tcatgggcat gaagccgcac
     3841 acccgggacg cgatccttcg gaaccgaccc gatatcacga tcaacaaacg gcggctgttg
     3901 cgtgatttga atgctatttc gagaattggt ttaggcgatc acggctcggt cactcgactg
     3961 gtcttttcga tcaaagaatt gcgcagccgt cagtttctga ttcaccaaat gcggcaaatc
     4021 ggtttgcaga tcaacatcga ccgcatcggc aacattttcg gaaaactcga gggttctgat
     4081 ccaaaagctc ctgctgtggt tgctggctcg caccttgaca ccgtcatcca gggtgggaag
     4141 ttcgatggta cgatgggagt cattgccgca cttgaagcag ctcggacgtt gaaagaaaac
     4201 aacgttccgc tgcgctcgcc cgttgaagtg gtttgttttg tcggcgaaga gtcgagccgc
     4261 tttggttttt caaccctggg gagcagcttg gttgcaggtg aagtgcggcc cgaggatttt
     4321 gttaacgcca gcgacctaca gggagctaaa ctagaggata ttttggctgg actgggcatt
     4381 catcggaaca acctcctctc gctgcgccgt gatccctcaa ctttgaaggc ctaccttgaa
     4441 ctgcacatag agcaaggccc gattctagaa gcaaggaaga aaccgatcgg cgtggtgaca
     4501 tcgattgcgg cgccgacacg gtttaaggta atcatcaccg gtcaggccga tcactcaggg
     4561 acaactccaa tggagatgcg aaaggacgct ctggtttcgg cagcggaatt aatcgtagcc
     4621 atagaaggaa tttgtcgcga gcattcggaa atggccaagg gccgggtggt cggtactgtg
     4681 ggcgcactca aaattgaacc cggagtgatc aatgcggtcc caggaaaggc ggaattagcc
     4741 gtcgacgtac ggagcatcac cgcgtcagcc aagaaccggg tcgttcgttt gatcgaagcc
     4801 aagattcgcg aggtcgcttc acgccgcaaa ataagaataa agattctacc tatacgaaaa
     4861 gaggaccccg tccctctgaa taaacgtgta atccatctgc tccaagactg ctgcgaggcg
     4921 cgggaggttc cttacgagat tatgccttca ggcgccggtc atgacgcaat gcagatggca
     4981 aaaattactt cggcggggat gttgtttatt ccaagccgtc gcggtattag ccataatccg
     5041 ttggaatggt ctgctcccga agacatctct ttgggcgcac aattactctt agacgctatc
     5101 gtacgagccg cgaatgaaga aatctaagaa cgtcaggctt ttgacggcgc ctgaaataaa
     5161 aaaaaacctt gagcggatgg ggcgggaaat tctccgacaa aataaagctt cggattcttt
     5221 ggccttcatc gggatccgca cccgtgggct ttttttagcc caacgggtaa gagccataat
     5281 ccaaaagttc cagcacaaag aaattcctct aggggagatg gacattagct tataccgaga
     5341 cgacctcaat gcgctgcttc cggaaggaaa agtcgaccat acgagcatcc ccttcgacat
     5401 taataacaaa acgatcattc tattcgatga cgtgctccac acgggtcgga cggtacgggc
     5461 cgcggtggat cacttgatcg acttaggcag acccaaagcc attcatctcg ccgtgctcat
     5521 agaccgcggc ggacgcgagt ttccgattgc cgccaatttc attgcaagaa agatcacttt
     5581 accccaaggc ggcgaggttc ggctaaaact caaggaagtg gacggcagag atgaagtggt
     5641 tgttggaaga tgacgaagta agaagctgta gggctcgtac ccgacggaac tagaatcgca
     5701 gttgagcaat cagagatggt ctgcgctcat catacgagat gctataagat gtctgttgca
     5761 atgctcggat atcagaaact agtgcgtgca ggaagcagac tgttcaacct tcaaatggcg
     5821 acgcatcacc aaagctagaa catcgcaggg ttcacggtga gaatcgataa atcgccgcag
     5881 gtcttccaat tctctgggtt ggaaatacga cgtgcatttg gtcataagcg attcgatgat
     5941 ctcagtcggt agtaatccaa acgtgacggt gtcgtcttcg gaacaaaagc cggccacctc
     6001 aatcgactct ggttctacgc ccgtgaccgc atccctgaac agctgaagga acagatcaat
     6061 tttccggtct tcgaattc
//
LOCUS       DASUTG010000451         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1699683, whole genome shotgun sequence.
ACCESSION   DASUTG010000451 DASUTG010000000
VERSION     DASUTG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699683"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF089_10335"
     CDS             complement(<1..661)
                     /locus_tag="VF089_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6174403.1"
                     /translation="MRRPQTIETIGVIGAGTMGHGIAQVAAGAGFRVVLRDVDRESLA
                     RGVKAIERNLAKGIELGKLTEADCDSTLQHIHGTTHLEELAGADLVIEAAPEILEIKQ
                     NLLREVEGIARKSFIFATNTSSLSISEIARASQYPANVVGLHFFNPVHLMRLVEIVVG
                     KATSDDTVATVREVSRKLRKEPIVVNDVPGFASSRLGVALGLEAMRMLEQGVASARDI
                     DT"
     gene            868..1989
                     /gene="trxB"
                     /locus_tag="VF089_10340"
     CDS             868..1989
                     /gene="trxB"
                     /locus_tag="VF089_10340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6174404.1"
                     /translation="MDKTVNPKQKIEREVVIIGSGPAGLTAAIYSARANLRPLLIDAP
                     PDAEKQTTPGGQLMITTDVENYPGFADGIQGPDLMVEFRKQAERFGTEFLEAWINKVD
                     LSERPFTLETDDHQISTQTLIIATGASAKWLGIPGEAKVPDGFGGNGVSACATCDGPL
                     PSFRNKPLVVVGGGDTAMEEATFLTRYASKVYVVHRRDKLRASKIMQDKATQNEKIEF
                     VWDTGVEEILGKPEEGVTGVRLRNLKTGDESVLPCSGVFIAIGHKPNTDLFKGQIDMD
                     EIGYILTSGKSTATNVPGVFAAGDVQDNCYRQAVTAAGTGCMSALDAERFLDHLPVAM
                     PSGEELTMEGEHVTADRTAIIEPDGRMIPNQPQMAAQED"
BASE COUNT          495 a          576 c          591 g          434 t
ORIGIN      
        1 cggtatcaat gtctcgcgca ctggcgacgc cttgttcaag catccgcatc gcctcgaggc
       61 caagtgcgac tcccaaacga ctggaagcaa agccgggaac gtcgttaacg acgatcggtt
      121 ccttccgcag cttgcgactt acttcgcgaa cagtggccac tgtatcatca ctcgtagcct
      181 tgcccaccac aatttccacg agacgcatca ggtgaacggg gttgaagaag tgcaggccaa
      241 ccacgtttgc cgggtattga gacgcccgtg caatctccga tatggaaagc gacgaagtgt
      301 tcgtcgcgaa gatgaaactc ttcctggcaa tcccttccac ttcccgaagc aggttttgct
      361 ttatctctag aatctcgggc gccgcttcga taaccagatc cgcgccagca agttcctcga
      421 gatgggtagt gccgtgaata tgttggagcg ttgaatcgca gtcagcttcg gtgagtttgc
      481 cgagttcgat acccttagcc agattgcgtt cgatcgcttt gactcctcgc gccaggctct
      541 cccggtcgac gtcccgaagc acaacccgaa acccagctcc cgccgctacc tgggcaatac
      601 catgacccat cgtgccggcg ccgatcacac cgatggtttc gatcgtttgc ggacgtctca
      661 agcttgtgtt caaaattcta tcagtggagt cgatctaagt aaggcttccg actctcgcgt
      721 tcgactacat tagtatcgct agttatacta tgacccaacg gaaaaggcta tacattcggc
      781 cattttgtag gcctatatta caccaatgca acccgcttga ctgcattccg gaagttgact
      841 caacgagcgg gctagctacc acaatgaatg gataagacgg tgaaccccaa gcaaaaaatc
      901 gaaagagaag tcgtcatcat cggttccggc ccggcaggac taacggcagc gatttattca
      961 gcgcgcgcaa atctgcgacc cctgctgatt gatgcgccgc ctgacgcgga gaaacagacc
     1021 actcccggcg ggcaattaat gattaccact gacgtggaaa actaccccgg cttcgccgat
     1081 ggcattcagg gcccggactt gatggtggaa tttcgaaaac aggccgaacg cttcggcacc
     1141 gaattcctcg aggcatggat caacaaggta gatttaagcg aacgcccttt tacattggaa
     1201 acagacgacc atcagatcag cacccaaacg ctgatcatcg cgaccggcgc gtccgccaag
     1261 tggctgggca ttcctggcga agcaaaagtt ccggacggct ttggtggaaa cggcgtttcg
     1321 gcctgtgcta catgcgatgg tccattacca agtttccgca ataaaccgct ggtggttgtt
     1381 ggcggcggcg acacggcgat ggaagaggcg acgtttttga cgcgttacgc gtcgaaagtt
     1441 tatgtggttc accgccggga caaactgcgt gcttcgaaga tcatgcagga caaggcgacc
     1501 cagaacgaga agatcgagtt tgtctgggac accggggtgg aggaaattct cgggaagcct
     1561 gaggagggcg taactggagt ccgcctgcgc aacttaaaaa cgggcgacga atcggtgctg
     1621 ccgtgctccg gtgtcttcat tgccattgga cacaaaccaa atactgatct gttcaagggc
     1681 cagatcgaca tggatgagat cggatacata ctcacgtccg gtaagtcaac agcgacgaat
     1741 gtaccgggcg tgtttgcggc cggcgacgtg caggacaact gttaccggca ggcagttacc
     1801 gcggctggta ccgggtgtat gtcagcgctc gacgccgaaa ggttcctcga tcatttaccg
     1861 gtggcgatgc ccagtggtga ggaactaacg atggaaggtg aacacgttac ggccgaccgt
     1921 acagcgatca ttgagcccga tggccgcatg attccgaacc aaccgcagat ggctgcgcag
     1981 gaagactgac cgttacaaca acacgattga atcgtcgcgg cgccggcgat cgatggcggg
     2041 aactggacgc gaggctggct ctaacggcgt gacggagggg tttcgcgggc aggctt
//
LOCUS       DASUTG010000452         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1254320, whole genome shotgun sequence.
ACCESSION   DASUTG010000452 DASUTG010000000
VERSION     DASUTG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254320"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="proS"
                     /locus_tag="VF089_10345"
     CDS             complement(<1..836)
                     /gene="proS"
                     /locus_tag="VF089_10345"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX6174405.1"
                     /translation="MRWSQILIPTLKEDPSDAEVISHKLLVRGGFIRQVSRGIYDYFP
                     LALKVIRKIENIVREEMDRAGAQELLMPIASPAELWQESGRWDVYGKELIRFKDRNDR
                     DFCLGPTHEEIVTDLVRRAVRSYRELPLNLYQIQTKFRDEVRPRFGLMRGREFIMKDA
                     YSFHADIDDCKREYDNMVLTYRRIFDRCGLKYRPVEADTGAIGGSLSHEFQVLAESGE
                     DAIVSCDSCDYAANLEKAAVKAHRPPARDLTAEAPAFTKISTPGKKSVTEVAEFLELA
                     PE"
     gene            complement(875..1969)
                     /gene="ispG"
                     /locus_tag="VF089_10350"
                     /gene_synonym="gcpE"
     CDS             complement(875..1969)
                     /gene="ispG"
                     /locus_tag="VF089_10350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX6174406.1"
                     /translation="MKRRTTRQIKLGSVKVGGDAPITVQSMTKTDTCDVRATVHQIWS
                     LEAAGCDIVRVAVPVRAAAEQLAEIKKKIRIPLVADIHFNYKLALIALEQGVDGLRLN
                     PGNIGARRYVEEVVRLAAERETPIRIGVNAGSLEKDLLEKAGGPTVEGMVESGLRHIR
                     ILEDLNFSDIKISLKASDPLMMIEAYRMLADKVDYPFHLGVTEAGTPTVGTIKSAVGL
                     GTLLAEGIGDTIRVSLAADPVEEVRVGAEILKSLGLKKEGLTFVACPSCGRADVDLVG
                     LAKAVEERMVPYNNLNIHVAVMGCEVNGPGEARAADLGVAGGKGIGLLFKKGQVIRKV
                     PEARIVDALMEEVQKLADEKKAEQPAAADD"
     gene            complement(2063..2875)
                     /gene="jag"
                     /locus_tag="VF089_10355"
     CDS             complement(2063..2875)
                     /gene="jag"
                     /locus_tag="VF089_10355"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6174407.1"
                     /translation="MDYVETEGDTIDQAIDNALVRLGVERDRISIEILSEGKRGILGF
                     GTQKARVRASLRRSALEPTPLERAPDPVVVASAAPKPLTEAEIAAMGEKAKGVLSEIL
                     KRMGIEASVQAKIDHEADEIVLEVVCDDSGLLIGRKGQTLEALQYLVTRIAGDRQGSE
                     GPHILVDVENYRQRRKKSLEDMALRLGEKAKRQRKTVTVDALSAADRRIVHAALQDDP
                     WITTKSLGQGSYRRLLIIPEGDRRRKEESKPLDKQAPKFQSNTDRPGPTRTS"
     gene            complement(2899..>3795)
                     /gene="yidC"
                     /locus_tag="VF089_10360"
     CDS             complement(2899..>3795)
                     /gene="yidC"
                     /locus_tag="VF089_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX6174408.1"
                     /translation="KEFSGQLSWAGFGFTYFMMALVPENGAGYRASVQQSGPALMLAI
                     AEPTGNPNPSRYTFFVGPKELSVLESIGNGMEKAIDFGYFGFISIPFLYILHFFHQFT
                     RSYGIDIILLTVLLKILTAPLTHKSYASMKQMQKLQPQMERLKEKFKDDREKLNKEIM
                     EMYRRNGVNPLGGCLPMVVQFPVFIGLYNALYIPIELRHAPFLWINDLSKPDWQSLPF
                     EFAGWHLGVPVLTILMGASMFIQQWMTPTAGDPNQRKLMLMMPLIFTFMFVTFPAGLT
                     IYWLINNVLSIAQQWWINRSVK"
BASE COUNT          814 a         1048 c         1016 g          917 t
ORIGIN      
        1 ttctcaggcg caagctcgag aaattcggcg acttcggtca cgctcttttt tccgggagtg
       61 gagatcttcg tgaaagcggg tgcttctgcc gtgagatcgc gcgctggcgg tcgatgtgcc
      121 ttgactgcgg ccttttcaag attagctgca tagtcgcagc tatcgcagct aacgatggca
      181 tcttccccgg attctgccag gacttggaac tcatgcgaca agctgccgcc gattgcgcca
      241 gtgtcggctt ccacaggccg gtacttcagg ccgcagcgat cgaaaatgcg ccgataagtc
      301 aggaccatgt tatcgtattc gcgcttgcag tcatcgatgt cagcatgaaa gctgtaggca
      361 tccttcatta taaactctcg tccgcgcatg aggccgaagc gcggacgaac ttcgtcgcga
      421 aatttggttt gaatctgata aagattaagc ggcaactcgc gatacgatcg cacggctcgg
      481 cgcaccaggt cggtaacaat ttcctcatgg gttgggccta agcaaaagtc tctgtcgtta
      541 cgatctttga agcgaatcaa ctccttgccg taaacatccc aacgcccgct ttcttgccag
      601 agttcggcgg gcgaagctat cggcatcagg agctcttgcg cgccagcgcg gtccatttct
      661 tctctgacga tgttttctat tttgcgaata acttttagag cgagcgggaa atagtcgtag
      721 ataccccggc tgacctggcg gatgaagccg ccgcgcacga gcagtttgtg actgatgact
      781 tcggcgtcgg atggatcttc cttgagtgtt ggaatgagta tttgactcca gcgcatatag
      841 tccttgggag agaacgagat tgttgcaggg acgactaatc gtcggctgcg gcaggctgct
      901 cggctttttt ctcatcggcg agcttctgca cttcttccat gagggcgtcg acaatgcgag
      961 cttcgggcac cttgcgaatc acctgacctt tcttgaacag taggccgatg cctttgccgc
     1021 cggcaacacc caaatcggca gcccgagctt cacccgggcc gttgacctcg cagcccatga
     1081 ctgcgacgtg gatattcagg ttgttgtaag gaaccatccg ttcttcgacc gctttggcga
     1141 ggcccacgag atcgacatcg gctcgaccac aggaaggaca agcaacgaaa gtcagacctt
     1201 ccttttttag acccaacgat tttagaatct cggcgcccac ccgcacttct tcgaccggat
     1261 cggccgccaa agaaacacgt atcgtgtcgc caatgccctc agcaagtaga gttcctaggc
     1321 ctactgcgga tttgatggtg ccgacggtcg gcgtgcctgc ttcagtgacg cccaagtgaa
     1381 acgggtagtc gactttgtcg gcgagcatgc ggtaggcctc gatcatcatc agcggatcgg
     1441 acgctttaag tgaaattttt atatcggaaa aattcaaatc ctctaagatg cggatatggc
     1501 gcaacccgct ctcgaccatg ccctcgaccg ttggaccgcc ggctttttcc aataagtcct
     1561 tttccaagga accagcattg acgccgatac gaatcggtgt ctcgcgctcg gcggcgagcc
     1621 tgacgacctc ctcgacataa cggcgcgcgc cgatatttcc ggggttgagg cgtaaaccat
     1681 ccacaccctg ctccaaagcg attagcgcga gtttatagtt aaaatgaata tcggccacca
     1741 gaggaatgcg gattttcttt ttgatctcgg cgagttgttc ggctgccgcg cggactggca
     1801 cggcaactcg gacgatatcg caacccgctg cctccagtga ccatatctga tgaacggtgg
     1861 cgcggacatc acaagtatcc gtcttggtca ttgactgaac ggtaataggt gcgtctccgc
     1921 ccactttgac gctaccgagc ttgatctgtc ttgttgtccg gcgtttgatc accgattcac
     1981 tataggaaac tggcttggag cggtcaaaca aagattgaag gttgccgctc gtgcagacgg
     2041 aaacgctgag tcagcagccg gcttaggagg ttcgggtcgg gccgggcctg tcggtgttgc
     2101 tctgaaattt cggagcttgt ttgtctaggg gtttcgactc ttctttcctc ctacgatccc
     2161 cttcaggaat gatgagcagc cggcggtagg atccttggcc aagacttttg gttgtgatcc
     2221 atggatcatc ctgtaaggcg gcgtgaacaa tgcgccgatc agccgcgctc agagcatcga
     2281 cggtgacggt ctttcgctgt cgtttggcct tttcaccgag gcgcaacgcc atgtcttcga
     2341 gcgatttttt ccgccgttgg cggtaattct cgacgtcgac gaggatgtgc ggcccttcgc
     2401 ttccttgccg gtcgccggcg atgcgtgtca cgaggtactg caaagcttcc agggtttgac
     2461 ctttgcgacc gatcagcagg ccgctatcgt cgcagacgac ttctaacacg atctcgtcgg
     2521 cttcgtggtc gatttttgcc tgcacggaag cttcgatccc cattcgtttc aggatctcac
     2581 tcaggacacc tttggccttt tcgcccatag cggcgatttc cgcctcggtt aaaggtttgg
     2641 gagccgcgct tgcgacaacc acgggatccg gtgcccgctc gagtggggtc ggttcaaggg
     2701 cagaccttct tagtgaggca cgaacccgcg ctttttgggt gccaaagccg agaatgccgc
     2761 gctttccctc cgacaatatc tctatgctaa ttctgtcacg ctcaacgccg agacgtacca
     2821 gcgcgttgtc gattgcctga tcgatggtgt ccccttcggt ttccacgtaa tccatagact
     2881 atacacctcg ctaacagcct acttcacgct gcgattaatc caccactgtt gggcgatcga
     2941 aagaacgtta ttgatcaacc agtagatagt tagccccgcc ggaaaggtta cgaacataaa
     3001 ggtaaatatc aacggcatca tcagcattag cttccgttga ttcgggtcgc ccgctgtggg
     3061 ggtcatccat tgctgaatga acatgctcgc ccccatcaat atcgtaagca cgggaacacc
     3121 cagatgccaa ccggcaaatt cgaacggcag cgattgccaa tcaggcttgg aaaggtcatt
     3181 tatccacaaa aatggcgcat ggcgcagttc gatgggtatg tacagcgcgt tataaaggcc
     3241 gataaaaact ggaaactgca cgaccatcgg caaacaaccg ccgagcgggt tgaccccgtt
     3301 gcgccggtac atttccatga tctccttgtt cagtttttcc cgatcatcct taaatttctc
     3361 cttcaaccgt tccatttgtg gctgaagctt ctgcatctgc ttcatggaag cataactctt
     3421 gtgtgtcagc ggcgccgtca atatcttgag cagcaccgtc agaaggatga tgtcgatccc
     3481 atagctccga gtgaattggt gaaagaaatg gagaatgtat aggaacggga ttgagatgaa
     3541 tccgaagtag ccaaagtcga tggccttctc catcccattg ccaatagact ccaggacgct
     3601 caactctttc ggcccgacaa agaatgtata acgactaggg ttgggattgc cggtcggttc
     3661 ggcgatggcg agcatcagtg ccggtccgga ctgttgcacg ctcgctctgt accccgcgcc
     3721 attctctggc acgagcgcca tcataaagta ggtaaatccg aatcccgccc aggaaagctg
     3781 gccggagaac tcttt
//
LOCUS       DASUTG010000453         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1996434, whole genome shotgun sequence.
ACCESSION   DASUTG010000453 DASUTG010000000
VERSION     DASUTG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996434"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VF089_10365"
     CDS             <1..645
                     /locus_tag="VF089_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX6174409.1"
                     /translation="QQVYPDMAEFFADLARVYREEIAALAEAGCTYLQIDDTNLAYLC
                     DPKMRDNARKIGEDPEKLPALYAGLINDCIKDRPPSMTVCTHLCRGNFASAWVAEGAY
                     DPVAEVLFNELKVDGYFLEFDSPRAGNFAPLRYLPPGKRLNLGLVTTKTGALEKADEL
                     KRRIDDASKIVPLEQLGVSPQCGFSSTVLGNKLTIRDQVAKLNLIVEVAKDIWN"
     gene            766..1077
                     /locus_tag="VF089_10370"
     CDS             766..1077
                     /locus_tag="VF089_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6174410.1"
                     /translation="MVRKLKSIGLVIVLLGLLGGCQAMTGRTAGENVDDATITTTVKA
                     RLAQDKLGSLTRVDVDTTNGVVALNGVVATAQDRSRAEEVARGVGGVKKVTNNLQVQK
                     K"
     gene            complement(1201..2196)
                     /locus_tag="VF089_10375"
     CDS             complement(1201..2196)
                     /locus_tag="VF089_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174411.1"
                     /translation="MVAKVAFIIGLLASFLVMPGRQVFAASTPITSMRVLYPSFGGTW
                     ATPWIAKEAGYFSAEGLDVELIRVGGSTRMVAAMLGGSAPIIQAGAAAAIAATAAGSD
                     VVIIAATGNVSPFRLMARPEIKQPLDLKGKRAGITTFGSTSDQVVRIALQKFNLEPVK
                     DVAILSMGAQPEAFAALQSGAVQVAALSYPLYAKAAQLGMRELINFGDLGVEDVNGGV
                     ITTRSFIAQHRDTATRFIRAYIRGAHRYRTDKEFSKRVLGKYGKITEDDLLEGTWQDY
                     APTIQKVPRPSPKAVQFLIEFQFKGKTPLPKPENFVDTSIVDELEKNGFIDSVYK"
     gene            2666..3808
                     /locus_tag="VF089_10380"
     CDS             2666..3808
                     /locus_tag="VF089_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6174412.1"
                     /translation="MSEHDTARIDHLLSLLAQQSKEHAFVLLDANARVLWWSPGAGYI
                     FDRSSSEMVGQPLGILFTPEDRERGIPDQEVQLAVNFGKAEDDRWQIRRDGSRFWATG
                     ILIGLRDHNRNLVGFGKILRNRTDLREQLEALRNQVQALTAANQRKDNFLATFSHELR
                     NPLASICTTVELISRTASGNANLQRSVKLIEEQLDFVRRMLDDLLDVTRISTGKVRLN
                     VRPVVLQEIIEKAVEAARPRVEERYQRIQVIGPRSAIVLQGDADRLHQVFSNLLSNGV
                     KYTPERGAICVEVTIEADEAVVRVSDTGVGIPHDMLPGIFDLFTQVETQLSHGGLGIG
                     LALVKDFVALHGGSVQVRSEGVGKGSEFTVRLPLNIQRTDAELRIS"
     gene            3863..>4294
                     /locus_tag="VF089_10385"
     CDS             3863..>4294
                     /locus_tag="VF089_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174413.1"
                     /translation="MKLGLQIPSFTWPAGTSNLASTLARIGQAAEEAGFASLWVMDHF
                     FQIDMIGKPEEPMLEGYSALNFLAAVTKRIRLGTLVSGVIYRHPGILIKTITTLDILS
                     GGRAYFGIGAGWYEREAIGLGVSFPPLKERFERLEETLQIAL"
BASE COUNT          963 a         1137 c         1212 g          982 t
ORIGIN      
        1 cagcaggtct atcctgatat ggctgagttc tttgccgatc tggcccgtgt ttaccgcgag
       61 gaaattgccg cgctcgctga agccggctgt acttatctgc aaatcgacga caccaattta
      121 gcttatctgt gcgatccaaa aatgcgcgac aatgccagaa agatcggcga agatcccgaa
      181 aaattgcccg ccctttatgc tggcctgatc aacgactgta tcaaagatcg accgccatcg
      241 atgaccgttt gcacccacct gtgtcggggt aattttgcca gcgcctgggt tgccgaaggg
      301 gcctatgatc cggtcgccga ggtgctgttt aatgagctta aagttgacgg ttattttctg
      361 gagttcgatt cgccacgggc cggaaacttt gcgccgctgc gatatctacc gcctggcaag
      421 cgactgaatc ttggtctcgt gaccactaag acaggggcgc tggagaaagc cgatgagctc
      481 aagcgccgca tcgatgatgc gagcaagatc gtgccgttgg agcaactcgg cgtcagtccc
      541 cagtgcggtt tctcgagcac ggtcctgggt aacaagctga caattcgaga tcaggtcgcg
      601 aaattgaatt tgatcgtcga agtagccaaa gatatctgga attagagagc tttcgaggca
      661 cctagtctag ttcccctcga agcgaacgac catgtccatc gcacgcatgc accctacttg
      721 cttcaatcat ccgaggttgt tagcgtacga aaggaggact caactatggt acgaaagcta
      781 aaatctatcg gccttgtgat cgtgctgctc gggcttctcg gtggatgtca ggcgatgacc
      841 ggccggacag ccggcgagaa tgtcgatgac gctaccatca ccacgacggt gaaggctcga
      901 ctggcccaag ataagttagg cagcttgacc cgtgtcgatg tcgataccac caacggtgtg
      961 gttgccctaa acggagtcgt tgctacggcg caggacagat cgcgtgcgga agaggtagcg
     1021 cgcggcgttg gtggcgtaaa aaaagttacc aacaatctcc aggttcagaa gaagtagcag
     1081 ccgtaggatg ccagtgactg ctaggcggcc tcgcgtgagt tggtgcgcgg ggccgcatct
     1141 tcggcgaatg acctttcttc gcccacgcct attaccggcc gctatccgcc gagctgcggg
     1201 ctacttgtac accgaatcga taaagccgtt cttttcgagt tcgtccacga tagacgtatc
     1261 gacaaagttt tccggtttcg gcagcggtgt cttgcccttg aactgaaact caatcaaaaa
     1321 ctgaacggcc ttgggcgagg ggcgtgggac tttttgaatc gtcggcgcat aatcctgcca
     1381 agtgccttcc aaaagatcat cttcagtaat cttgccgtac tttcctagaa cgcgtttgct
     1441 aaactccttg tcggtacgat agcggtgcgc gccgcgtatg taagcccgga taaaacgggt
     1501 ggcagtatcg cgatgctgcg caataaaaga ccgggtggtg atgaccccgc cgttgacgtc
     1561 ttccactcca aggtcaccga agttaatcaa ctcgcgcatc cctagctggg cggccttcgc
     1621 gtagagtgga taacttaacg ccgccacttg cacggcaccg ctctgtaatg cggcaaacgc
     1681 ctccggctgg gcgcccatcg acaaaatcgc cacatcttta actggctcca aattgaattt
     1741 ctgcagcgct atgcgaacca cctgatccga agtcgatccg aacgtggtga tgccggccct
     1801 tttccctttt aggtctaaag gctgcttgat ctcgggtcgc gccatcaaac ggaagggcga
     1861 tacgttgccc gtggcggcga tgataacaac atcgctgccg gcggccgtgg cggcgatcgc
     1921 cgcagcagcg cctgcttgaa tgatcggcgc gcttcctccc aacatcgcag ccaccatccg
     1981 ggtgctgccg ccgacccgaa tgagttcgac atcgagtcct tcggcggaaa agtagccggc
     2041 ctctttggcg atccagggcg tcgcccaggt gccgccaaag ctagggtata gaacccgcat
     2101 cgacgttatt ggcgttgatg cggcaaaaac ttgtctgccg ggcatcacaa gaaaagaagc
     2161 cagcaatccg ataataaaag cgactttcgc taccatggta atcttatctc ctgagtgatg
     2221 attcgttgcg atcgagtttg aagaataaag tttagatttc tatgtcggtc tcgatacgcg
     2281 acgatttaac agctcgtcgg gaactaatgc aaactcccgt gtgtttgttc tgatggcggg
     2341 tagttgcctc gcgggcaaat accagttcgc tagaccttga gcatgttcta gcaatggtcg
     2401 cttaccttgc ggatttttgg ctgctccgaa gagctagatc aagccgatac agcaccttag
     2461 ccggcgaagc ccgttcgctt aggatagata gctcaatgag actcagaggc gatttttcgc
     2521 ccggagagct tacaaatttg tttgcaactg aatctggagt tgcgtgagat cttcggtata
     2581 cttggcagtg gtatcttaca tcgcggattc tttgacgacg gcgccttaac ccagcacagc
     2641 ttccttcaac aaagggacct actgaatgtc ggaacatgat acagcgcgca tcgatcacct
     2701 tctaagtctt ctggctcagc agtccaaaga gcacgctttt gtgcttctgg acgcgaatgc
     2761 ccgcgtgctg tggtggagcc ctggggccgg gtatatcttc gaccgcagct caagcgagat
     2821 ggtaggccag cctcttggaa ttctgtttac tccggaagat agagagaggg gaattcccga
     2881 tcaggaagtt cagctagcgg tcaatttcgg caaggcggaa gatgatcgat ggcagatccg
     2941 tcgagacggc tctcggtttt gggcgaccgg catactgata gggctgcgtg atcacaaccg
     3001 gaatctcgtc ggctttggca agatccttcg taaccgaacg gatctgcgcg agcaactcga
     3061 agcattgcgc aaccaagtac aggcattgac tgcagcgaat cagcgcaagg ataacttttt
     3121 agccactttc tcccatgaac ttcgcaatcc gttggcgtcg atatgcacta cggtggaact
     3181 catcagtaga acagcctctg gaaacgccaa tttacaacgt tcggtcaaac tcatcgagga
     3241 gcagctggat tttgtccgca ggatgctcga tgacttactg gacgtgacgc gaatcagcac
     3301 gggcaaggtt cgcttaaatg tccgacctgt cgtgcttcag gaaattatcg aaaaggctgt
     3361 ggaggcggcg cggccgaggg tcgaagagcg ttatcaaaga atccaggtca tcgggccgcg
     3421 gtccgcgatt gtgctgcaag gcgatgctga tcgattgcat caggtctttt cgaatctact
     3481 gagtaatggg gtcaagtaca ccccggagcg cggcgctatc tgcgtcgagg tgaccatcga
     3541 ggctgacgag gccgtggtcc gggtcagcga caccggagtg ggaattccgc acgatatgct
     3601 gccggggata ttcgacctat ttactcaagt ggaaacccag ctttcccatg gcgggctcgg
     3661 catcggcctg gccttggtca aagactttgt agcccttcac ggcggttcgg ttcaggttcg
     3721 cagcgagggc gttggtaaag gcagcgagtt tacggtgcga ttaccgctca acattcagcg
     3781 cacagacgcc gagctgcgaa tctcctaaac gttctggcga aatcgccacg aaggtcggtt
     3841 cagcgttcgc ccggagggca tgatgaaatt gggactacag ataccgtctt ttacttggcc
     3901 ggcggggaca tcgaatctag cgtcaactct ggctcgcatc ggtcaagccg cggaggaagc
     3961 cggatttgca agtctctggg tgatggatca tttttttcag atcgacatga tcggtaagcc
     4021 cgaagagcca atgttggaag gctatagcgc gcttaacttt ctggcggccg tgaccaagcg
     4081 aatccgcctt gggacactcg ttagcggggt gatttaccgt caccctggta ttctcatcaa
     4141 aaccataacg actctcgata tcctatccgg aggccgcgct tacttcggca tcggggcggg
     4201 ctggtacgaa cgcgaggcta tcgggctggg cgtttccttt ccgccgttga aggaacgctt
     4261 tgaacgttta gaggaaaccc tccagatcgc tctg
//
LOCUS       DASUTG010000454         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1700543, whole genome shotgun sequence.
ACCESSION   DASUTG010000454 DASUTG010000000
VERSION     DASUTG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700543"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VF089_10390"
     CDS             <1..1160
                     /locus_tag="VF089_10390"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX6174414.1"
                     /translation="FKVKFCYAQQTRIHFIGVWDTVDAVGLPFHVSDLINAVIYRFKF
                     PNRRLSEQVDHAYHALAVDDDRHSFHPLLWDQGAAKPGKLQKIEQVWFAGAHANVGGG
                     YPKQGLSLTAMHWLMTKAQNAGLRFIPGEYEYYRDHANVDDKLYDPRAGMGVFYRWKP
                     RDIAQLCRANGVTPAIHLSVMERIAHGTEDYSPGNLPTDARVVITPNGDQEKDVTAMT
                     RALAAETILKSAHANTQSSLLNCVRGTISVGRLSYYVYLLSCIAALLAASGALDDPSR
                     TGIWAITKALALFLVDLVASPFATLYAVGQNFSGNLIRVAWLASGFLLAYLLMKITDK
                     RMTGVFSKFWFEQQKDLRLALKQARQKADEIISTGVYSSQPATDCNKKASG"
     gene            complement(1237..2280)
                     /locus_tag="VF089_10395"
     CDS             complement(1237..2280)
                     /locus_tag="VF089_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174415.1"
                     /translation="MKQRIVLGLLLFSCFYFGSVSTALAQESFYKGKSIRLIVGSTPG
                     GFYDRWARFFARYMPKYIPGNPEIAVQNMPAAGSLVAANYVYNVAKPDGLTLGMVQYN
                     IYMDQLVGRKEVQFDVRKFNWIGSPASESVLLYMRKDAPYQSIRDIIKAKEPPKCGSS
                     GTVSTDYLLARLLEDAIGAKFNTVIGYPGGSEIDLAVEKNEVVCRAHNISAHFGREPF
                     NSWHKKDFDRHIIQTGRKRDARLPDTPTIYELFDEYKSPDASRRVAQVILTGGDLGRP
                     MLITPGTPPDRVKIFREAYIKALKDPELLLETEKAKMDVDPTPGEELEELIKKVMDQP
                     KEVVDRVKKMLGN"
     gene            complement(2320..3447)
                     /locus_tag="VF089_10400"
     CDS             complement(2320..3447)
                     /locus_tag="VF089_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6174416.1"
                     /translation="MKIADIEIIELQNIPVTPPLFKQPVRTAVRLLKVKTDDGRIGIS
                     QLGGFMHSATAAFIQQDLLPFLKGKDPLENERLMHQMLWKFNTRAHAGVWNFAASAID
                     VALWDLKGKFYSAPVWRLLGGAQKAIPSYITFGLRAYNRDELAEAAKHWVANGQSRLK
                     IQVGRSNIRGEMDPSGASGEHREDNPAEDESRIRAVREAVGDDVELMADANCLMKYDA
                     ALRWCKRFEPYNLMWFEEPIIHNDPHLLAELRKQTSIPIAAGQWDNFFKLADLVKQNS
                     VDFLNIHVLSVGGFTMGMKAAGLAHAFNLPVGNGDHFDIHLHAAVPNGWRAEIHVNNW
                     LTANVVYKDLPAPVKGWITLTEKPGLGLELNDDAIKEFQVR"
     gene            complement(3557..>4646)
                     /locus_tag="VF089_10405"
     CDS             complement(3557..>4646)
                     /locus_tag="VF089_10405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174417.1"
                     /translation="ARNFDLVCGAQANFEVLGGHVKKLLLSLSILIASVTELQAQAPF
                     YQGKTITFLVGSGSGTAYDIYSRLLAAYIGKQLPGNPTVVVQNMPAAGGLVAANYVYG
                     VAKPDGLTLASINPAHYFNQLQGNKEVKFDWTKFAWIASMDKSEHMLYMRSDTPFKTM
                     QDVRKAAEPPKCGATGTGTSGHYIPRMLEETLGTKFTIVTGYAGGNEIDLATERNEVV
                     CRSFTTQAYFQREPYHTWRKKNFVRILMQTGRTRDPRLPETPLLSELMDEVKTPDLGR
                     RVATVMLGSGELGRPVVTSPATPPDRIQLLRDAFMKSMNDPALLEEAKQKKLDITPVS
                     GEELAKIAREVIDQPQDVVERIRKILAQ"
BASE COUNT         1032 a         1312 c         1200 g         1102 t
ORIGIN      
        1 aattcaaagt caaattttgt tacgcccagc aaaccaggat tcacttcatc ggggtctggg
       61 acactgtcga cgcagttgga ttgcctttcc acgtcagcga cctcatcaac gcggtaatct
      121 accgattcaa gtttccgaat cggcggttga gtgagcaggt agaccacgcc tatcatgccc
      181 tggctgtaga cgacgaccgg cattcgtttc atcccctgct ctgggatcag ggggctgcca
      241 aaccaggcaa attgcaaaag attgagcagg tgtggtttgc cggcgcccat gccaatgttg
      301 gcggcggcta tccgaagcaa ggcttatcgc tcacagcaat gcactggttg atgaccaagg
      361 ctcaaaacgc agggctgaga ttcatccctg gcgagtacga gtattatcga gatcacgcca
      421 acgttgatga taaactttat gacccgcgtg ccggcatggg tgttttctac cgctggaaac
      481 cgcgcgatat cgcgcagctc tgtcgtgcta acggcgtgac tccggcaatt cacctgagcg
      541 tgatggaacg gatcgcgcac ggaacggagg actacagtcc gggaaatcta ccgacggacg
      601 cgagggttgt tatcacgccc aacggcgacc aagaaaaaga tgtgacggcg atgacccggg
      661 cacttgctgc agaaactatc ttgaaatctg ctcacgcaaa cacccaaagc tctctgttga
      721 attgtgtccg cggcaccatc agcgtcggcc ggctctccta ttatgtttac ctactgtcct
      781 gcatcgcagc cttgctcgcc gcctcggggg cgctcgacga cccctcgagg accggcatat
      841 gggcaattac caaggcacta gctctcttcc tggtcgatct agtagcctca ccgttcgcga
      901 cgctctatgc ggtgggacag aacttttcag gtaacctcat ccgggtcgcc tggctagcga
      961 gcggctttct cctggcttac ctgctgatga agatcactga taaacgaatg accggagtat
     1021 tttcgaagtt ctggttcgaa caacagaagg atttacggtt agccctgaag caggcgcgtc
     1081 aaaaggcaga cgagatcatt tcaaccggtg tctattcatc gcagccggca acggattgca
     1141 acaaaaaagc cagcggttaa gttcttcatg gatcatcgta catacgctct gcttacctct
     1201 ttaccctgag cgagatctct cagccatccg catgcttcag ttgccgagca tctttttgac
     1261 tcgatcgacg acttcttttg gctggtccat cactttcttg atcagctctt ccaactcttc
     1321 gccgggtgtc ggatcgacat ccatcttggc cttttctgtt tcaagcagaa gctccggatc
     1381 cttcaacgcc ttgatataag cttcgcggaa tattttcaca cggtccggcg gggtgcccgg
     1441 agttatcagc atcggccggc ccagatcccc gccggttagt atcacctgag cgacgcgccg
     1501 gctggcgtcc gggctcttgt attcatcaaa cagttcatag atcgtcggcg tatccgggag
     1561 gcgtgcatcc cgcttgcggc cggtctgaat tatgtgacga tcaaaatctt tcttgtgcca
     1621 gctattgaac ggttcgcgac caaaatgtgc tgatatgttg tgggcgcgac agacaacttc
     1681 attcttttca accgcaagat ctatctccga gccgcccgga taacctatta cggtattgaa
     1741 cttcgctcca atcgcatcct cgagcaatct cgctagcagg tagtcggtgc ttacggttcc
     1801 ggatgagccg catttcggcg gctccttggc cttgatgata tctcggatcg attgataggg
     1861 ggcatccttg cgcatataga gcagcacgct ttcgcttgcc ggagaaccga tccagttaaa
     1921 cttccggacg tcaaactgca cttccttacg ccctaccagc tggtccatgt agatgttgta
     1981 ctgcaccatt cccaatgtga gcccgtcggg cttggcgaca ttgtagacgt agtttgctgc
     2041 aaccagagac cccgccgcag gcatgttctg cacggcgatc tcaggattgc cgggaatata
     2101 tttgggcatg tatcgggcga agaatcgcgc ccagcggtca tagaaacctc cgggggttga
     2161 gccgacaatc aaccggatag acttgccttt gtagaaagat tcttgcgcca gggcggtcga
     2221 gacagaaccg aagtaaaaac agctgaacaa aagcaaacca agcactatgc gctgcttcat
     2281 gactcctcca caaatttgtg cttcacgcga agacccaatt tatcgtactt gaaactcttt
     2341 aattgcatca tcgttaagct ccagaccgag tcctggcttt tccgttaaag ttatccatcc
     2401 tttaaccggt gccggcaagt ccttatagac gacgtttgcc gtcagccagt tgtttacatg
     2461 aatctcggcg cgccagccgt tgggaacagc ggcgtgcaag tgaatatcga aatgatcgcc
     2521 atttcctacc ggcagattga acgcgtgcgc cagtcccgcc gccttcatgc ccatggtaaa
     2581 cccacccaca gaaagcacgt ggatgtttaa gaagtccaca gaattctgct ttaccaggtc
     2641 ggcgagcttg aaaaaattat cccactggcc ggcggcgatc gggatgctcg tttgtttacg
     2701 gagctcggct aataagtgcg gatcgttgtg gataattggt tcttcgaacc acatgagatt
     2761 ataaggctcg aagcgcttgc accatcgcag cgcggcatca tacttcatca aacagttggc
     2821 atcggccatc aattctacat cgtcgcccac cgcttcgcgc accgctctga tgcgagactc
     2881 atcttccgcc gggttatctt cgcgatgctc gccgctggcg ccggaaggat ccatctcgcc
     2941 acgaatattc gagcggccga cttgaatctt cagccggctc tgtccgttgg cgacccaatg
     3001 cttggccgct tcggcgagct cgtcacgatt ataagcacgc aacccgaagg tgatgtagga
     3061 cgggatcgct ttctgcgcgc cgcccaacag acgccacaca ggggcgctgt agaacttccc
     3121 ttttagatcc cacagcgcaa catcgatcgc gctcgcggcg aaattccaca cgccggcatg
     3181 ggcgcgggtg ttgaatttcc agagcatctg atgcatcaag cgctcgttct ccagcggatc
     3241 cttgcccttt agaaatggca atagatcctg ttggataaag gcagccgtcg ccgaatgcat
     3301 gaaaccaccg agttgggaaa taccgatccg cccgtcgtcg gttttgacct tcaacaaccg
     3361 cacggccgtg cgcaccggct gcttgaacaa cggcggcgtc accggaatat tttgcagctc
     3421 aatgatttcg atatcagcta ttttcaaagt ggttctcctt tagaattttt tcgcaaattg
     3481 ttaagaacag cagtatgata gggggcacgc gaagttgatc ctggcgaatc aacagagtac
     3541 ccaactccgc aatatcctac tgcgccagta tcttccggat acgctcgacc acatcctgcg
     3601 gttggtcgat cacctcgcgg gcgatcttcg ccagctcttc gcccgacacg ggagtaatat
     3661 ccagcttctt ttgtttcgct tcttccagca gcgccggatc attcatgctt ttcataaagg
     3721 catcgcgaag taactggatt cgatccggcg gcgtcgccgg cgatgtcacc accggtcgtc
     3781 cgagctcgcc ggaaccgagc atgaccgtcg ctactcgtcg tccgagatcc ggcgttttga
     3841 cctcgtccat gagttcgctt aacagcggcg tttccggcaa ccgcggatcg cgcgtccggc
     3901 cggtttgcat aagtattcgg acaaagttct tcttgcgcca ggtatgataa ggctcgcgct
     3961 ggaaataggc ctgagtcgta aacgaacggc aaacgacttc gttgcgctca gtggcgagat
     4021 cgatttcatt gccgccggca taaccagtga cgatggtgaa tttagtgcca agagtttctt
     4081 cgagcatccg agggatgtag tgcccgctgg ttccggttcc cgtggcgccg catttgggcg
     4141 gttcggccgc cttgcggaca tcttgcatgg tcttgaacgg cgtgtcgctg cgcatgtaga
     4201 gcatgtgctc cgacttgtcc atgctggcga tccaggcaaa cttcgtccag tcaaatttta
     4261 cttccttatt gccctgaagc tgattgaaat agtgcgctgg attgatagaa gccagtgtca
     4321 gcccatcggg cttggcaact ccgtaaacat aattagcggc aaccaatccc cccgccgcag
     4381 gcatattctg cacgacaaca gtggggttgc ccggcagttg cttcccgata tacgccgcca
     4441 acaaccgcga atagatatca taggcggtgc ctgagccgga gccgacgaga aatgtaatcg
     4501 tcttaccttg atagaacggc gcttgggctt gcagttcggt aaccgaagcg atcagaatag
     4561 aaagcgatag cagcaacttc ttcacatgac ctcctagcac ttcaaagttg gcctgagcgc
     4621 cgcatactaa atcgaagttt ctcgca
//
LOCUS       DASUTG010000455         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1700643, whole genome shotgun sequence.
ACCESSION   DASUTG010000455 DASUTG010000000
VERSION     DASUTG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700643"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF089_10410"
     CDS             complement(<1..505)
                     /locus_tag="VF089_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174418.1"
                     /translation="MKPLMSESKDNSSLEALGRASVQIVHDLKNQINGLKLYATFLRK
                     RSERSERPADEIETINKLITGLDRTAADLSMIVELGQPLQLNRHAGVNLTNLLQKVTA
                     NVNDSPRVTGALKGPVLIEVDQQPLAGEFDAPKLEQAFKAISASALKLLGSKTPDASL
                     NVVLRRVT"
     gene            682..1131
                     /locus_tag="VF089_10415"
     CDS             682..1131
                     /locus_tag="VF089_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174419.1"
                     /translation="MAKLNQTGHTGHSGRDVGGSGETPTILLVEDTEDNRHMMKKLLE
                     MSGYRVLEATNGEEAVQVTSKESPELILMDLSLPIIDGLAATRLIRNLPEGNHLPIIA
                     VSAHDTADFHADALAAGCDAYITKPINYPELEEVVARLMSAPKTKTQ"
     gene            1225..1731
                     /locus_tag="VF089_10420"
     CDS             1225..1731
                     /locus_tag="VF089_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174420.1"
                     /translation="MPFSDQQVSEPDGSRSKIIIIVAVVAAILLAILFYFLMRASTTR
                     PPDPLLPGAIRAGSPEWAASASKIHLDAPEATESKRALGDIVMTLQTTARNFTGRTIT
                     GLEIKGSVVNRQGQPVRERTIVVIPTQQQPELEPNRTMLVVIGLEGMTDADERANIRM
                     EVTGFTIR"
     gene            complement(1745..2665)
                     /locus_tag="VF089_10425"
     CDS             complement(1745..2665)
                     /locus_tag="VF089_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174421.1"
                     /translation="MSHVVEIENLTKDYEVGFVRKRKVRALDGLSLKVNQGEIFGFLG
                     ANGAGKTTTLKLLMTLIFPTAGSARILNQDIADVHMHRKIGYLPENPYFYDYLTAFEF
                     LTFCGQIFGYSKTERTSRAKQLLARVGLDESKWNTQLRKFSKGMLQRVGLAQAIINDP
                     EVVFLDEPMSGLDPIGRREVRDLISELRGEGKTVFMCSHILSDIEVLCDRVAIMKRGR
                     LSHLGALEDLGQQGSGVEVIVTGVSAESLSKFLPQSLQFQLTPTPAGLRIEVPGEKNV
                     DEVIGALRKADGMLVAVQPLKQSLEELFLN"
     gene            complement(2674..3582)
                     /locus_tag="VF089_10430"
     CDS             complement(2674..3582)
                     /locus_tag="VF089_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174422.1"
                     /translation="MISSKRYTNWIPASVLVISVIGYVLIARWLDDHRPKLHPEIQQE
                     SLYLTGNALKRMSLGFNGLAADWYWMRALQYVGGKILSAQKPIQLDDLSQLDMKLLAP
                     MLDVATTLDPAFMEPYLYAAVVLPAIDEDEAIRITRKGIAANPNQWRLYQHLGYIYWQ
                     RGDYVSAGQAYTEGAKIAGAPIWMQAMKARMAAEGGSRDVAREIYGRMYEEAGDPKVK
                     DMARLRLLQLQSMDQRDVIRKLLEIFKTRTGRCPSSWQEMAPLLRAARLPIADGGPID
                     PGGTPYTLVTNACDVDLDPTSTIPHK"
BASE COUNT          890 a          988 c          944 g          935 t
ORIGIN      
        1 ttgtcacgcg ccgcaaaacc acgttaagcg atgcgtccgg agtcttgctc cccaataact
       61 tcaacgcgct agccgagatt gccttgaatg cttgctccag tttcggcgca tcaaactctc
      121 ctgctagtgg ttgctgatcc acctcgatca aaaccggtcc tttcaaagcg cccgtgaccc
      181 gtggactatc attaacgtta gccgtcacct tttgaagtaa gtttgtaaga ttcacgccgg
      241 cgtgcctgtt taattgcagc ggctgtccta attcgacgat catcgaaagg tcggcggcgg
      301 ttcggtccaa tcccgtaatc aacttgttga tggtttcgat ttcatcggct ggccgttcgc
      361 tgcgctccga tcgcttccgg aggaacgtgg catacagttt gagaccgttt atttgattct
      421 tgagatcgtg aacgatctgt accgaggctc ggcccagcgc ctccaaagag gagttgtcct
      481 tgctttccga cattaatggc ttcatctagc gttgtaacct tccttgaacc ggttgaccgt
      541 gctgttgggg gaggattgca gaccagcgca gtgtattgaa tagactcgga cagggggaat
      601 ccaatatgtc gtgtttcggt gcggtatatt agcaaaaata ggcggcgtat tctagtgata
      661 caggaacttt atgccacgga ggtggccaaa ttgaatcaaa ccggacatac gggacattcc
      721 ggacgcgacg tgggcggctc gggagaaacc ccgacgatcc ttttggttga ggataccgag
      781 gataatcgtc acatgatgaa aaaactcctg gaaatgagtg gatatcgggt actcgaggct
      841 acgaacggtg aagaagcggt ccaggtgacc tcgaaagaat cgccggagct aattctgatg
      901 gatttgagtc tgcccatcat cgatggcctt gccgccacta ggctgatccg taatcttccg
      961 gaaggtaatc accttcccat catcgctgtt tcggcgcacg acaccgcaga cttccatgct
     1021 gatgctctgg cggcggggtg cgatgcgtac atcactaaac ccattaacta tcctgagctt
     1081 gaggaagttg tagcgaggtt aatgtcggcg ccgaaaacaa agacacaata gttgagaaac
     1141 tttttgctgc ggtcgtggtc taagtatccg gcagactacg ttgcaaacta gcttcgacca
     1201 cactagaatt cagcccacgt atcgatgccc ttctcagatc aacaggtctc tgaaccagat
     1261 ggctcgcgaa gcaagataat tattatcgtc gctgtcgtag ccgccatcct actcgcgatt
     1321 cttttctatt ttctcatgcg tgcgagcacc acgcggccgc ccgacccgct gttacctggt
     1381 gcaatccgcg caggctcacc tgaatgggct gcatctgcat cgaagattca tctcgacgct
     1441 ccggaagcca ctgagtctaa acgggcgctt ggcgatattg taatgaccct gcaaacaacc
     1501 gcgcgtaatt tcactgggcg cacaattacg ggacttgaaa taaaagggtc tgtcgtcaac
     1561 cggcagggac agccagtgcg cgaacgaact atagttgtca ttccaactca acaacagccg
     1621 gagctcgaac caaatcgcac catgctggtg gtgatcgggc tcgaaggtat gaccgatgcc
     1681 gacgagcggg ccaacatcag gatggaagtg acgggcttta cgatcaggta ggtgattgct
     1741 tgatctagtt cagaaacagt tcttccaaag attgttttaa aggctgcaca gctaccaaca
     1801 taccgtccgc ttttctcaaa gcaccaatta cttcatcaac gttcttttcc ccaggaactt
     1861 ctatgcgcag cccagcaggc gttggggtga gttgaaactg aagactttga ggcagaaatt
     1921 ttgataaaga ttcagcggag acgccggtaa cgatcacttc gactccggac ccttgttgac
     1981 cgagatcttc caacgctccc aaatgcgata acctaccgcg tttcataatg gctaccctgt
     2041 cacacagtac ttcgatgtca gagagaatgt gggagcacat gaagaccgtt tttccttcgc
     2101 ccctaagttc ggatatcaga tcacgtacct ctcgcctccc aatcgggtcg aggccgctca
     2161 taggctcatc cagaaataca acttcggggt cattaatgat ggcctgggcc agcccaacgc
     2221 gttgtaacat tccctttgaa aactttctga gctgtgtatt ccatttgctt tcatcaagtc
     2281 caacccgtgc taacaattgt tttgcccggc tcgtgcgctc agttttcgag tatccaaaaa
     2341 tttggccgca gaaggtcagg aattcaaaag cagtgaggta atcgtagaag tatggattct
     2401 caggcaggta acctattttc cggtgcatgt gtacgtccgc gatatcctgg tttaatatgc
     2461 gagcgcttcc cgccgttggg aaaatgagtg tcatcagaag cttcagggta gttgttttac
     2521 cggcgccgtt tgctccgaga aagccaaaga tctctccctg attaaccttc agcgacagtc
     2581 catccagggc gcgcaccttc cgcttgcgga cgaaccccac ttcatagtcc ttagtcaggt
     2641 tctctatttc gactacgtga gacatctaat cagttacttg tgcggaatgg ttgaagttgg
     2701 atctaaatcg acatcgcagg cgttggttac taacgtatag ggtgtgccac ctggatcgat
     2761 tggcccacca tcagcgatcg gcagccgtgc agctcgcagc aacggcgcca tttcctgcca
     2821 tgaactcgga cacctgccgg ttcttgtttt aaagatctcg agaagcttac gaatcacgtc
     2881 tcgttggtcc atagactgca gttgaagcaa acgcagccga gccatgtctt tcactttggg
     2941 gtcgccagcc tcttcataca tcctaccgta gatttctcga gccacgtcgc ggctgccacc
     3001 ctcggctgcc atccttgctt tcattgcctg catccatatg ggtgctcccg cgatctttgc
     3061 gccctcggta tatgcctgac cagccgatac gtaatcgccg cgttgccagt atatgtaacc
     3121 gagatgctga tagagacgcc attgattggg attagcggct atgccttttc gcgtaattct
     3181 aatggcttcg tcttcatcga tagcaggtaa aaccaccgcg gcgtaaaggt acggctccat
     3241 aaatgcagga tccaacgtcg tcgctacatc caacattggg gcaagtagct tcatatcgag
     3301 ctggctgagg tcgtccaact gaatcggttt ttgagcgctc aagattttgc cgccaacgta
     3361 ttgaagtgct cgcatccaat accagtccgc agccaggccg ttgaagccca ggctcatgcg
     3421 cttcagcgca ttccctgtga ggtataaaga ctcctgttga atttcgggat gtagctttgg
     3481 acggtgatca tcaagccatc gagcaatcag aacatatcca atgacgctga ttacgagtac
     3541 tgacgccggg atccagttcg tatatctctt gctcgaaatc acgttatggg ctccgctact
     3601 taaagtttcg acgactgaaa atcaccatgg tcgctgcaag gaggactgca acatataaca
     3661 gtgcgtaaag cagcgatgcc gcgaagtgtg gcatccccgg gacgtgtcca tgcgctgcag
     3721 gggttacgaa actgtaattg gcaaaattag ggagcaa
//
LOCUS       DASUTG010000456         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_960655, whole genome shotgun sequence.
ACCESSION   DASUTG010000456 DASUTG010000000
VERSION     DASUTG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960655"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF089_10435"
     CDS             <1..612
                     /locus_tag="VF089_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174423.1"
                     /translation="ALFGAVYGSLKALGETRVPQVVAYAGVAFFSALWWSLGVTGNFT
                     LQPTIYGAAMILISAGLLLAWRSIRERYGNLTLNGMHGLAGPMPRFATALSLLVMAAL
                     GLLPFGLFSAYLGMLLQASAPVSWGLMVILFTWFLASWYLFRMMQRLLFGAHRSDIRY
                     NDFQASEVAVFALLLLSLIALGAMPLDWFETVLLARGSGIPWR"
     gene            615..3779
                     /locus_tag="VF089_10440"
     CDS             615..3779
                     /locus_tag="VF089_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEX6174424.1"
                     /translation="MGQLAATQYSETERMELRALVLLASEVIADYWPMRTFVHHNQLH
                     GLEYLHFEEAVSRARRLLDAKGYLSVEVFRDYFRSGRILPDQLDGVLKARAENRHVQL
                     AAHDVTLGAVLRACMLQDLSPPPADTFGTLLERHPNRTAIETLASHLLPAMKLPQSGP
                     GAAEQPYLGRTLMLASWCDRTLGTEIGEQINRELVKWCEAFLDEGHATWPMPRREKGF
                     YGAWRFLTQQEWSSGAITNSREKLARLPAQPEDALLESLGILGIPAEARQDYLSLHLV
                     ALSGWAGFIKWRADQSEYEWQLAYPIDLAQYLAVRLWYERELVQKACRESLGIAGNID
                     AISTEMQRRENSSAKAPGHAQADRPAALVAAWRLYALAQALGHDPGILTQSEPGNLLT
                     LLEWINGFPESDHGPIWLQAFEGGYQEELIGKLPVDFGQVSEVGSGQGPARRAGAQSE
                     LQDEIKVRHQAQAVFCIDVRSEPIRRHLEAIGDYETFGFAGFFAVFIRYQSLGSDHET
                     DQFPVIMKSKNLVREIPRTYQGQYLFRRRHREKLLDTGHTLLYDLKENVVTPYVMVES
                     LGWFYSLPLIGKTLSPTWYRNVVSWLRRKLVPPVATTLTVDKLSREEVEEMLAAEQRA
                     ITRRALQENFGERHLNLSLERLEFLRRRALYGESAGEPSPQARSTALSREEEAAFVED
                     LRRNYGINPGASFARMERITRTGFTVSEQVFTVETALRMMGLTRNFARLVLFCGHGST
                     SENNPFEAALDCGACGGNPGKANARVLAIMANKPPVREQLAKNGIAIPQDTYFIAGQH
                     NTTTDDIDLFDLEDLPPTHRKDLLQLVRDLELAGTLNSQERCARFPEIKVRLSAAKAR
                     RAVHRRSADWSQVRPEWGLSGNAAFIIGRRALTRGINLEGRAFLHSYDYREDFDGRLL
                     EVVMTGPQIVGQWINMEHYFSAVDPEHYGSGSKIYHNVVGRFGVMSGPQSDLRTGLAW
                     QTVMNGVRPYHEPMRLVTLVEAPRERIDKIILNQRHLKRLYDNQWVYLIAIEPEEQLV
                     YRYVPREGWQPVKPNCSSLAQRFA"
BASE COUNT          788 a         1051 c         1100 g          845 t
ORIGIN      
        1 gccctcttcg gtgcggtgta tggatcactc aaagctttgg gtgaaacgag agttccgcaa
       61 gtagtggcct acgccggcgt cgcctttttt tcagctttgt ggtggagtct tggagtaaca
      121 ggaaacttta ctcttcaacc gaccatctac ggcgccgcga tgatactgat ttcggctggg
      181 ctgctcctcg cctggcggag catcagggag cggtacggca atctgacctt gaatgggatg
      241 catggtttgg cagggccgat gccgcgattt gctacggcgc tttctttgtt agttatggcg
      301 gctcttggct tgctcccgtt cggccttttt tctgcgtacc ttgggatgtt gctgcaagct
      361 tctgccccgg tctcatgggg attgatggtg attctattca cctggttttt ggcatcgtgg
      421 tatttgttca gaatgatgca gcggcttctg tttggtgcgc accgctccga tattcgctat
      481 aacgacttcc aagcaagtga ggtcgcggtt ttcgcgcttc tgctacttag tctaatcgcc
      541 ctcggcgcga tgccgctcga ttggttcgaa accgttctac tggcgcgcgg cagtggaatc
      601 ccatggagat gactttgggt caattggcag caacccagta cagcgaaacg gagcggatgg
      661 agctccgagc attggtcctg ctggcgagcg aggtcattgc ggattactgg cccatgcgga
      721 ctttcgttca ccacaatcag ctgcatggcc tggagtatct ccattttgaa gaggccgttt
      781 ctcgggctcg gcgattgctc gacgccaagg gttatttgtc ggttgaagtt tttcgcgact
      841 atttccgctc cggccgaatt ctgcccgatc aactcgatgg cgtgctgaaa gcgcgcgcag
      901 aaaacaggca cgtccaatta gccgctcatg acgtgactct cggggctgtg cttcgcgcct
      961 gcatgctgca agatctctct cctcctcctg ctgacacgtt cggtactctg cttgagcgcc
     1021 atccgaaccg caccgcaatc gaaacactgg caagtcatct gctaccggct atgaagctgc
     1081 cgcagagcgg gccaggcgcg gcagagcagc catatctcgg tcgaactcta atgctggcca
     1141 gctggtgcga tcgcaccttg ggcacagaaa tcggcgagca aatcaatcgt gagttggtca
     1201 agtggtgcga agcctttttg gatgaaggtc atgccacctg gcccatgccg cgccgtgaga
     1261 agggtttcta cggcgcttgg agatttctga ctcaacagga atggtcttcc ggagcaatca
     1321 cgaacagccg ggagaagctg gcccgactgc cggcgcagcc ggaagacgcg ttgctcgaaa
     1381 gtctcggaat tctcggcatc ccggccgaag cccggcagga ttatctctcg cttcacctcg
     1441 tggctctttc gggatgggcg ggcttcatca aatggcgcgc cgatcaaagt gaatatgaat
     1501 ggcagctcgc ctatccgatc gatctggcgc agtatcttgc ggtgcgcctt tggtatgaac
     1561 gtgagctggt gcaaaaagcc tgtcgtgaat cgctcggcat cgccggcaac atcgatgcaa
     1621 tttcgactga gatgcagcgt cgtgaaaact catctgcgaa agctcccggc cacgcgcagg
     1681 cggataggcc ggcagctctc gtcgccgcct ggcgattgta cgcactcgcg caagctctgg
     1741 gacatgatcc cggtattttg acgcaaagcg agcccggcaa cctgctgaca ttgctcgagt
     1801 ggatcaatgg gttccctgaa tcggaccatg ggcccatatg gcttcaggcg ttcgaggggg
     1861 gataccagga agaactaata ggcaaactcc ccgttgattt cggccaagtc agcgaagtcg
     1921 gttcgggtca gggtccggct cggcgagccg gggcacaaag cgagttgcag gacgaaatca
     1981 aagtccgcca tcaggcgcag gcggtgtttt gcatcgacgt gcgttccgag ccaatccggc
     2041 gtcacttgga agcaattggt gattacgaga ccttcggctt cgcaggtttt tttgctgtct
     2101 tcattcgcta ccagagcctc ggcagcgatc atgaaaccga tcagtttccg gtaatcatga
     2161 agagcaagaa tttggtgcgc gagattcccc ggacctatca aggtcagtat ttattcaggc
     2221 gccggcaccg tgaaaaactt ctcgataccg gacataccct gctctacgat cttaaggaaa
     2281 atgtcgtcac cccctatgtc atggtggagt cgctgggttg gttttacagc ttgcccctca
     2341 tcggcaagac cctttccccc acttggtacc gaaacgtcgt gagctggctt cggcgcaagc
     2401 tggtgccgcc ggtagcgaca acgttgaccg tggacaaact gtcacgcgaa gaagtagagg
     2461 aaatgctggc ggcagaacaa cgcgctatta cgcgccgcgc gttgcaggaa aacttcggtg
     2521 agcgccatct caatctatcg cttgagcgct tggagtttct gcgccggcgg gcgctctacg
     2581 gagagagcgc gggtgaacca tcgccgcagg cgcgctccac cgcgctttcc cgcgaggaag
     2641 aagcggcctt tgttgaagat ctcaggcgaa actacggcat caaccccggc gcgtcctttg
     2701 ctcgtatgga gcgtatcacc cggacggggt tcactgtgag cgaacaagtc ttcacggtgg
     2761 aaaccgcctt acggatgatg ggcctaacgc ggaatttcgc ccgtctggtg cttttttgcg
     2821 gccatggaag cacctctgag aacaacccct tcgaggcggc tctcgactgc ggcgcctgtg
     2881 gtggcaaccc gggtaaggcc aacgctaggg tgctggcgat catggcgaac aaaccgccgg
     2941 tgcgagaaca gctggccaag aacggcattg ccattccgca ggacacttac ttcatcgccg
     3001 gacagcacaa taccaccacc gatgacatcg atctgttcga tctcgaagac cttcccccaa
     3061 ctcaccgtaa ggatctgctc caactagtcc gcgatcttga actggcggga acgctaaaca
     3121 gccaagaaag gtgcgctcga tttccggaga tcaaagtgag actgagcgcc gcgaaagccc
     3181 gacgagcggt gcatcgccga agcgccgact ggagccaggt gcggccggaa tgggggctgt
     3241 ccggtaatgc cgcgtttatc attgggcgcc gcgctttgac ccgcggcatc aatctcgagg
     3301 ggagggcttt tctgcattcc tatgattacc gtgaagattt cgacggccgt ttgctcgaag
     3361 tcgtaatgac cggacctcaa atcgtcggcc aatggatcaa tatggagcat tacttttccg
     3421 cagtcgaccc ggagcactac ggcagcggca gcaaaatcta tcacaatgta gtcggccgct
     3481 ttggcgtcat gtccggtcca cagagcgatc tccgcaccgg gctggcctgg caaacggtca
     3541 tgaatggagt gcggccctat catgaaccta tgcggctcgt gaccctggtg gaagcaccca
     3601 gggaaaggat cgataaaatc atcctcaatc aacgccattt gaaacgactg tacgataacc
     3661 aatgggtata tctgatcgcc atcgagcctg aagagcagct cgtttatcgt tacgttccga
     3721 gagaaggatg gcagccggtg aaaccaaact gttcatctct tgcccagaga ttcgcctgaa
     3781 agga
//
LOCUS       DASUTG010000457         4252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_71186, whole genome shotgun sequence.
ACCESSION   DASUTG010000457 DASUTG010000000
VERSION     DASUTG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71186"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VF089_10445"
     CDS             <1..978
                     /locus_tag="VF089_10445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174425.1"
                     /translation="EFRLGDGTVLRPRTERIQSGEEKVAKHHTEGPERGYDPVSQVEA
                     MEREGLDLAVLFRTSPLYADDSFEPEYAAALCRAWNSWIADFCKHDPVKLKPSAVIPM
                     HDVDLAVKETRRAVKELGAIGLSIGPEPVRGRQIHDRYFDPLWAEAQDLDVAVCFHPP
                     ASPGSEQASKRFEGHPNDKILVNAFRNPVEQMFAVGSMCGGGVLERFPRLRTSFLEGN
                     CSWLPWTLYRLDERFELAGELADSPLKMKPSEYFQRQCFISVDVDEELIVDTVRLLGA
                     DCFVISTDYPHIDSKWPHALDSFLAIDGLDKDSQEKILWDNCARLYGLS"
     gene            complement(1077..1376)
                     /locus_tag="VF089_10450"
     CDS             complement(1077..1376)
                     /locus_tag="VF089_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174426.1"
                     /translation="MPFKFMPLYVTVDYHDVSDIVLVTQKHHQVAACGNGIGNMGRAD
                     ETDVNLTGEHRLGSPASDNEHRFDLDAVFAKKASFARHPGCRHVGIDGAVGEYGF"
     gene            1360..1983
                     /locus_tag="VF089_10455"
     CDS             1360..1983
                     /locus_tag="VF089_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174427.1"
                     /translation="MNLKGKRIAIGASQAGLASLATHAALDHMGLSARRDNIALLLIG
                     EEPLRLAALQAGNVQATLIAPELANTATSQGFSILLDMAKLNIPFQASGMVTTRKLMR
                     SDPLMLERVGRATADAIKFIVAPPNKKTVLQIMSKNLKLAKPDRLEASYNDLVEELPR
                     SICPTVAGVRSVMKFMAELGLNPKAAEFKVDDVVDLTLCKRLGGDGL"
     gene            2097..3137
                     /locus_tag="VF089_10460"
     CDS             2097..3137
                     /locus_tag="VF089_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174428.1"
                     /translation="MNTKPNVLAGVLALLVSLSYCATSLAAAVDGQAAAIQVESEVMW
                     YTTMALDDSSVLVEKFKAKYPKVNVKLFRANNSKILERILAESRAKSAIVDVVSAGGL
                     PIGVLKERGMLAKYISREAVAFPTGARDGDGFWTDIYVNTFNLVYNPTMVSAQDVPKS
                     YTALLDDKWQGNIGLDDSDYEWFGNILQIMGEERGLNFMRRLAALKPAMRAGHSLIAT
                     LVAAGEFAIYPDGYPRRVEVLRERGARSIDWLWLEPAIAELHPTAIMANAPHPNAARL
                     FTDYLLSAEGQELIGVQFGRVPSRKGVKLKYPRMTLEGRNIHWSNPTIMAKQGVRYAE
                     LFQSIFGKSSGQ"
     gene            3330..>4252
                     /locus_tag="VF089_10465"
     CDS             3330..>4252
                     /locus_tag="VF089_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174429.1"
                     /translation="MKSNFILMTILLTFMIARPAAGEIINFSTASDSGNTAPLWVAAD
                     LGFFEKYGNTVRLVFIQGATVSISALLNNDVHMAQFSPMLAIANNIKGIDFTITMSFN
                     QFMDNNVFGRKGIADVKEIKSLAIGRYGSSSDFMGRFLLQREGLKPETEVALLQFGNQ
                     TGRLLAIEAGRADAAIVTPPITLMARKKGFPLLIDASRLKIPYTSAVVVTRKSFIQAN
                     RPVVVNFMKGLIEAICYYKTHKEQSFKVMSKYMRIQDREVLEENFRAYDHSLRPYATD
                     AELEIPIQEVSKSEPKAVKANPAQFVDHSII"
BASE COUNT          995 a         1077 c         1209 g          971 t
ORIGIN      
        1 gagtttcgtc tgggcgacgg caccgttctg cgtcccagga cggaacggat ccaaagcggc
       61 gaagaaaagg tcgcaaagca tcacactgaa gggcctgagc gcggctatga cccggtctcg
      121 caggttgaag ccatggaacg tgaggggctg gatctcgcgg tgttgtttcg gacctcgccg
      181 ctctacgccg atgatagttt cgaaccggag tacgctgcgg cgttatgccg ggcttggaat
      241 agctggattg cggacttttg caaacacgat cccgtgaagc tgaaaccttc tgccgttatc
      301 ccgatgcacg atgtcgattt agcagtcaag gaaacccggc gcgctgtgaa ggagttgggc
      361 gctatcggtc tttcgatagg cccggagccc gttcgtggcc ggcagataca cgatcggtac
      421 ttcgatccat tgtgggcgga ggcccaggat ctcgatgtcg cagtttgctt tcatccgccg
      481 gcgagccctg gtagcgagca ggcgtccaag aggttcgaag ggcatcccaa tgacaagata
      541 ttagtcaacg catttcgcaa tccggtcgag cagatgttcg ctgtcggaag tatgtgtggc
      601 ggcggtgtct tggaacggtt tccccgcctt cgaacgtcat ttttggaggg caactgtagc
      661 tggctgccgt ggacgcttta tcgtctcgat gagcgtttcg aattggccgg ggaactagcc
      721 gatagcccgc tgaagatgaa gcccagtgag tatttccaac gccagtgctt catctcggtc
      781 gatgttgacg aggaactgat cgtagatacg gttcggctac tcggagcaga ctgtttcgtg
      841 atctccactg actatccgca tattgattcc aaatggcccc acgcgctgga cagcttcttg
      901 gctatcgatg ggttggacaa ggattctcaa gagaaaattt tgtgggacaa ttgtgcgcgt
      961 ttgtatggat tgtcctgagc agtggagggt gatcagtgac taaagtcgcg cgaatttccg
     1021 cattggcttt aactctttgg gcatcggcag cgagtgcttg ggcggccgac ggatcgtcaa
     1081 aagccgtatt cgcctacggc gccatcaatg cctacatgac gacaacctgg gtggcgcgcg
     1141 aacgaggctt ttttcgcaaa cacagcgtcg aggtcgaacc ggtgttcatt atcgctagcc
     1201 gggctgccca agcgatgctc gccggtgagg ttcacgtcgg tctcatcggc ccgacccatg
     1261 ttaccaatgc cgttgccgca ggcggcgacc tggtgatgtt tctgggtaac cagaacaatg
     1321 tccgatacat catggtagtc caccgtgaca taaagcggca tgaacttaaa gggcaagaga
     1381 atcgccatcg gagccagcca ggcgggctta gcatcattgg cgactcatgc ggcgctcgat
     1441 cacatgggac taagcgcgcg ccgggacaac atcgcgctcc tgctgatcgg cgaagaacct
     1501 ctacgattgg cggcgttgca ggctggcaac gtgcaggcga ctctgattgc accggaactg
     1561 gcgaacacag ccacaagcca gggctttagc atcctgctcg atatggccaa gctcaacatt
     1621 ccatttcaag ctagcggtat ggtgaccacg cgcaagctta tgcgttccga tccgctcatg
     1681 ctcgagcggg tcggcagggc gaccgcggat gccatcaagt tcatcgtagc tccgcccaat
     1741 aagaaaacag ttctgcagat catgagcaaa aatcttaagc tcgccaagcc ggatcgactc
     1801 gaagcgtcct ataacgatct tgtcgaagag ttgcctcgta gtatctgccc aactgtggca
     1861 ggcgtgcgtt cggtgatgaa gttcatggcc gaactcggcc ttaatccaaa ggcagccgaa
     1921 ttcaaggtcg atgacgtcgt cgatctaacg ctatgtaagc ggttgggagg agacggcctg
     1981 tgagaaccca gcgtctatca cgtgttgcgt atcaggtggg aactctagct acgaactttg
     2041 ctagaaacca tcgacgggcc tgtctctagt ccttttgact aaaagcggga gtgacgatga
     2101 atactaaacc aaacgtgctg gcaggagttc ttgccctgtt agtttctcta agctactgcg
     2161 cgacgtcttt ggcggctgcc gtggacggtc aggcagcagc cattcaagtc gaatccgaag
     2221 tcatgtggta caccaccatg gcgttggatg atagctcggt cttggttgag aagtttaaag
     2281 ctaaatatcc caaagtaaac gtcaagcttt tccgagctaa caactctaaa attttagaaa
     2341 ggatccttgc cgagagccgt gcgaagagcg cgattgtcga tgtcgtgagt gccggcgggt
     2401 taccaatcgg agtgctgaaa gagcgcggta tgcttgcaaa gtatatctct cgagaggcgg
     2461 tcgcgtttcc aaccggcgcg cgtgatggag acggcttttg gaccgacatt tacgtcaata
     2521 ctttcaatct cgtttacaat ccaacgatgg tatccgcgca ggatgtgccg aagagctata
     2581 cagcactcct agacgacaaa tggcaaggca acatcggtct cgatgattcc gactatgaat
     2641 ggttcggtaa tattctgcag atcatgggcg aagagagggg cctcaacttt atgcgcaggc
     2701 ttgccgccct gaagccggcg atgcgggccg gccactcgct gattgcaact ctagtagcag
     2761 caggtgaatt cgcaatttac ccagacggtt accctcggag agtagaagtg ctgcgagaac
     2821 ggggtgcgcg aagcatcgat tggctctggt tggagcccgc gatcgcggag cttcatccaa
     2881 ccgccatcat ggccaatgcg ccgcacccaa acgccgcgcg cctatttacc gattacctgt
     2941 tatcggctga agggcaagag ttgatcggag ttcagttcgg acgtgttccc agccgcaaag
     3001 gcgtaaagct aaaatatcct cggatgacgc ttgaagggcg gaacatccat tggagtaatc
     3061 cgaccatcat ggccaagcag ggtgttcgat atgcggaact ctttcaatcg atatttggca
     3121 agtcgagcgg ccaatagacg ctcgacatca gcgcatactc gctggacggc gacttacaat
     3181 caatcaaaat aagattcttg gtttatcggt ggtgttcaaa acctgacttt ccagcgtgat
     3241 cggcggccgg ggcagcaaat cagaagatcg gcggtgtgtc agaaatcggg gacatagcgg
     3301 gctaacagca atctggagta gagttctcta tgaaaagcaa ctttattctc atgacgatac
     3361 ttctaacgtt catgattgcg aggccggctg ccggtgagat tatcaacttc agtactgcga
     3421 gcgactccgg caataccgcg ccgttatggg tagcggcgga tctgggcttt tttgaaaaat
     3481 acggcaatac ggttcgcctt gtgttcatcc agggcgccac ggtttctatc tccgctcttc
     3541 tcaacaacga tgtgcacatg gcgcagtttt cgccgatgct ggcgattgcc aacaacataa
     3601 agggaatcga tttcacgatc accatgagct tcaatcagtt catggacaac aacgtctttg
     3661 gtagaaaagg aatcgctgac gtcaaagaga tcaaaagtct ggcaatcggt cgctatggca
     3721 gctcgtcgga cttcatgggg cgcttcctgc tgcagaggga gggcttgaag ccggaaactg
     3781 aggtcgcctt actccagttc ggaaatcaaa ccggccggtt gcttgccatc gaagctgggc
     3841 gcgccgatgc tgctatcgtc acgccgccga ttacgttaat ggcgagaaaa aagggatttc
     3901 ctcttctgat cgatgcctcg cgtttaaaga ttccgtacac cagcgccgtg gtggtcacga
     3961 gaaagtcttt cattcaagcc aaccggccgg ttgtggtcaa tttcatgaaa ggcttgatcg
     4021 aagccatttg ctattacaag acgcacaagg agcaaagctt caaggtgatg agcaaataca
     4081 tgcgcatcca ggatcgagag gttttggagg aaaactttcg cgcttacgat catagtcttc
     4141 gtccgtatgc tacggatgcg gaattggaaa ttcccattca agaggtcagt aagtctgagc
     4201 ccaaggcggt taaagcgaac cccgctcaat ttgtcgatca ctcgattatc aa
//
LOCUS       DASUTG010000458         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_514872, whole genome shotgun sequence.
ACCESSION   DASUTG010000458 DASUTG010000000
VERSION     DASUTG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514872"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VF089_10470"
     CDS             complement(<1..134)
                     /locus_tag="VF089_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX6174430.1"
                     /translation="MADQEKVQEQDAKKISRRNVLKAAVAAGSAAALGFPMIVKAQGP
                     T"
     gene            404..1840
                     /locus_tag="VF089_10475"
     CDS             404..1840
                     /locus_tag="VF089_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6174431.1"
                     /translation="MDKKNLVRQLCAIVGERYVLVEKEDVIVYEQDGSIFQVVPEIVV
                     LPGKVEQVAAIVKAAKQASVPIIPRGSGTGLAGGAVPAEGGIVLSLARLDRILRIDLE
                     NGLAIVEPGVINLDLTKAVTNDGFFYAPDPSSQAACSIGGNVANNSGGPHTLAYGVTT
                     NHVLGLEIVLDDGQIVWLGGEVPDTPGYDLPGVFVGSEGTMGVVTKVIVKLMHHRESV
                     RTLLAIYDRMDDATRAVVEITGAGIIPAALEMMDRTTIEAVESGAPVGFPRDAEAVLL
                     IEIEGLREQTDRSLQLAHAICEQSGAREVRVARDETERQRLWKGRKGAFGAMGSLAPN
                     YYVNDGVVPRSQLPEMMRRVAAISKQFKLRIANVFHAGDGNLHPNILFDMRAPGELDR
                     VIEAGAAIMSACVELGGSITGEHGVGIEKKAYIGMLYSAADLEAMTRVRTALDPDGRF
                     NPAKLFPSPAGCGEVRRQPAQIPAGLWI"
     gene            1859..3169
                     /locus_tag="VF089_10480"
     CDS             1859..3169
                     /locus_tag="VF089_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX6174432.1"
                     /translation="MEGILHNLQHGDQPKIATLDIPELYQCVHCGLCLNQCPTYRVLR
                     LEPESPRGRIHLVKAAADGRIELNDRFKDHLYLCLLCRACETACPSGVQYGHIAETAR
                     AVIGPPGTKLGRAILNLAFTQLLPFPGRLKILFGLLRLYQRTGLQRLVQPLLPRKIRQ
                     MDAMLPAIPRHRFQTTADALSPLGLRRARVAVLNGCVMPLLFGDINEATVRVLRRNGC
                     EVVFPKGQVCCGALNTHNGETSSAKDMARRNIDAFLTAGVEAIIVNAAGCGAAMKEYG
                     HLLEDDPVYADKARSFSSLVKDAGEFLAQIGLVGKLGELPMTVTYQDPCHLAHGQRVR
                     SQPRQLLQAIPGLKLVEMNGSDRCCGSAGIYNITHAEMSQQLLKEKMPDIADTGSQAV
                     VAPNPGCMLQLRYGAQKYGPDVKVYHLMDLLDRAYASGESSPRQ"
     gene            3166..>3435
                     /locus_tag="VF089_10485"
     CDS             3166..>3435
                     /locus_tag="VF089_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6174433.1"
                     /translation="MKLNPDRLARRLETEVGGATVTRHPDLLAARAVDEVYPCVICSP
                     ADAEEVSATLRVCSETDAAVTPWGGGTAIKLGNLPERVEVIIDLSK"
BASE COUNT          723 a          969 c          998 g          745 t
ORIGIN      
        1 gtcggcccct gggctttgac gatcatcggg aatccgagcg ccgccgcact gcctgcggca
       61 acggccgctt tgagaacgtt tcgccgtgaa atcttcttgg cgtcttgctc ctggactttt
      121 tcttggtcag ccatggtcag cccctttcag ttaagcacga ctactcgcgt tgacggctta
      181 tattacatat tacggattta cgtcaatggt acttccgggc tccactactc gcaggaattg
      241 agaagttaca attcgtatgt ctctctcctt cagcctcgtg ccactcgaaa attgaccccg
      301 agtaaagggc ctccttggcg cgagcgagtt ctttgatgtc gctgattgac actgtattcc
      361 actctccgct acgcttccaa cgtgtcccgg gagatagata caaatggata agaagaatct
      421 agtccgccaa ctatgtgcca tagttggcga gcgttatgtg ctggtcgaga aggaagatgt
      481 catcgtttac gaacaagacg gctcgatctt ccaggtcgtg cccgaaatag ttgttctgcc
      541 cggcaaggtc gaacaggttg ccgctattgt aaaagcggcc aaacaggcca gtgtgccaat
      601 cataccgcgc ggctcaggca cgggcttggc tggcggtgcc gtaccggccg agggcgggat
      661 tgtgctatcg ttggcgcgct tggatcgcat tttaagaatc gacctcgaga acggccttgc
      721 tatcgttgaa cccggagtta tcaatctcga cctcaccaag gccgtgacca atgacggctt
      781 cttctatgcg cccgatccta gcagccaagc cgcttgctcc atcggcggca atgtcgccaa
      841 caattccgga gggccgcata ctttagccta cggggtgaca accaatcacg ttcttggtct
      901 cgagatcgtt ctggacgatg gtcagatcgt ttggctcggc ggcgaagtgc cggacacgcc
      961 cggctatgat cttcccggtg tatttgttgg ctcggaagga accatgggtg tggtgaccaa
     1021 ggtgattgta aaactcatgc accaccgcga gtcggtgcgc acacttctcg ccatttatga
     1081 ccgaatggat gacgccactc gagcggtggt tgaaatcacc ggcgctggaa ttattcccgc
     1141 cgcattagag atgatggacc gcacgaccat tgaagccgtc gaatccgggg cgccggtagg
     1201 ctttccccgc gatgccgaag ccgtgctgct gatcgaaatc gaaggcttgc gcgagcaaac
     1261 cgaccgttct ttacaattgg cgcacgcaat ctgcgagcag agcggcgcgc gagaagttag
     1321 agtggcgcga gacgaaaccg aacgacaacg gctttggaaa ggcagaaaag gcgctttcgg
     1381 cgcgatgggt tcgttagcgc ccaattacta tgtcaacgac ggcgttgtcc ctcgcagtca
     1441 attgcccgag atgatgaggc gagtggctgc catcagcaaa caattcaaat tgcgcattgc
     1501 taatgtattt catgccggcg acggcaatct gcatccgaat attctgttcg acatgcgcgc
     1561 tccgggggag ctcgatcggg tcattgaagc gggcgcggca ataatgagcg catgcgtcga
     1621 gctcggtggc agcatcaccg gcgagcatgg cgtgggcatt gaaaagaagg cctatatcgg
     1681 catgctatat agcgctgcgg atctggaggc gatgacccgg gtccgcacgg cgctggaccc
     1741 ggacggccgg tttaaccctg caaagttatt tccatcgccc gcaggttgcg gcgaagtacg
     1801 gcggcagccg gcgcaaatcc ccgcggggct gtggatttag aaactgttgg agcttgtctt
     1861 ggaaggcatt ctgcacaatc tacaacatgg cgatcaaccg aagatcgcta cgcttgatat
     1921 ccccgaactc taccaatgcg tccactgcgg tctctgtcta aaccagtgcc cgacgtaccg
     1981 ggtgctgcgc ctcgaaccgg aatcgccacg cggcaggatt cacctggtga aagcggcggc
     2041 ggacggccgt atcgagctca acgaccgttt caaggatcat ctgtatctct gcttgttgtg
     2101 ccgtgcttgt gaaaccgcgt gtccgtccgg cgttcaatac ggccatatcg ccgaaacggc
     2161 gcgggcggta atcggcccgc cgggaacgaa gctcggacgg gctatcctga atctagcttt
     2221 cacacagcta cttccttttc ctggacgtct aaaaatccta tttggcctgc tgcgactgta
     2281 ccaacgaacg ggcttgcaaa ggctggttca gccgttgttg ccgcgcaaga ttcggcagat
     2341 ggatgccatg ctgccggcca ttccgcgaca tcgttttcaa acaacagctg acgctctttc
     2401 tccgctgggg ctaaggcgcg cccgtgtcgc ggttctaaac ggctgcgtga tgccgttgct
     2461 gtttggggac atcaatgagg ccaccgtgcg cgtgttgcgc cgcaacggct gcgaggttgt
     2521 tttcccgaaa gggcaagtct gctgcggcgc gctaaacacc cacaatggcg agaccagcag
     2581 cgccaaggac atggcacggc gcaacatcga cgcgttttta actgccggcg tcgaagccat
     2641 cattgttaac gcagccgggt gcggcgccgc gatgaaggaa tatggccatt tgctcgaaga
     2701 tgatcctgtt tatgcggaca aggcacgaag cttcagttct ttggtcaagg atgccggaga
     2761 attcctcgcg cagattggct tagtcggcaa actaggcgaa ttgccgatga ccgttaccta
     2821 tcaggatccc tgccatctcg cgcatggcca gcgcgtgcgc agtcagccgc ggcaattatt
     2881 acaggcgatt ccaggtttga aactggtcga gatgaatggc tctgaccgct gttgcggcag
     2941 tgccgggatc tacaatatta ctcacgccga aatgtcacag cagcttctca aagaaaaaat
     3001 gcccgacatc gccgacaccg gttcccaggc ggtcgtggca cccaatccag gctgtatgct
     3061 gcagctccgc tatggcgcgc agaagtacgg gccggatgtg aaagtctacc atctgatgga
     3121 tctcctcgat cgcgcctatg cgagtggcga gtcttcgccc cgccaatgaa gcttaacccg
     3181 gaccggctcg ccagacggtt agaaacagaa gttggcggcg ccacggtaac gaggcaccca
     3241 gacttgcttg ccgcccgcgc cgtcgacgaa gtttatccgt gcgtcatctg ttcgccggct
     3301 gatgccgagg aagtcagcgc gactttacgg gtttgcagcg agacggatgc cgccgtgact
     3361 ccgtggggcg gcggcacggc catcaagctc ggcaacttac ctgagcgtgt cgaagtcata
     3421 atcgacctgt ccaag
//
LOCUS       DASUTG010000459         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_960833, whole genome shotgun sequence.
ACCESSION   DASUTG010000459 DASUTG010000000
VERSION     DASUTG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960833"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF089_10490"
     CDS             <1..139
                     /locus_tag="VF089_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174434.1"
                     /translation="AGQIVVSGKPLGIIGEVHPEVLERWQIAVPVAAFDVNLSRLVEI
                     H"
     gene            complement(192..560)
                     /gene="rplT"
                     /locus_tag="VF089_10495"
     CDS             complement(192..560)
                     /gene="rplT"
                     /locus_tag="VF089_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6174435.1"
                     /translation="MPRAKGGPKTRARRKKRLKLAKGQYGAKSRLFRSATESVDKGQQ
                     YAYTGRKNRKRDFRQLWIARISAATRAHGLTYGRFINALKKANVLLDRKVLSDMAIKD
                     VAGFEQLVGLAKQHLTPATA"
     gene            complement(596..790)
                     /gene="rpmI"
                     /locus_tag="VF089_10500"
     CDS             complement(596..790)
                     /gene="rpmI"
                     /locus_tag="VF089_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6174436.1"
                     /translation="MKMKSHSGASKRFHRTGTGKFMRKRAGKRHILTSKNRERKRRLS
                     GSVVVDETKAAALKRLLPYT"
     gene            complement(823..1320)
                     /gene="infC"
                     /locus_tag="VF089_10505"
     CDS             complement(823..1320)
                     /gene="infC"
                     /locus_tag="VF089_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6174437.1"
                     /translation="MRVNREIRVREVRVIGPEGEQLGILPTPEAFKQAQEGGYDLVEV
                     APTSVPPVCRIMDYGKYKYELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIKIRQI
                     KGFLEEGNKTKVVLTYRGREMANQEMGRVVMNSVISELSESGTIEYAPRMEGRSLIMI
                     VAPKN"
     gene            complement(1343..3277)
                     /gene="thrS"
                     /locus_tag="VF089_10510"
     CDS             complement(1343..3277)
                     /gene="thrS"
                     /locus_tag="VF089_10510"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6174438.1"
                     /translation="MPKVTLKNGKSFDVPPGYTIAQALAAGGIPVGPEVLAASVNGRP
                     TDLSAPLTADSTIDPLGFDTAEGREIYRHSSTHIMAQAVKELFPSAQLTIGPALEDSF
                     YYDFAYDRPFTPEDLENIEQRAREIAKRKLAVTRRDLTKQEALELFRSRGEHYKVELI
                     EGFPDQEPISIYTQGEFVDLCRGPHVPSTGHVGALKLLTTAGAYWRGDERNPMLQRIY
                     GTSFPTKAELDAHLAKLEEIKRRDHRKVGKELDLISIQDEIGPGLVLWHPKGAVIRLL
                     IENFWREQHLRDGYELVYSPHVARLDLWKTRGHVDYYRDNMFAPMKLEGSEYQLKPMN
                     CPYHIMIYKSHLRSYRELPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLFCRPD
                     QIEAEVSRVLDFTFFILRTFGFMEFEVFLSTRPNESVGAEEHWTLATSALEAALKGRN
                     ISFHLDPGGGAFYGPKIDIKIKDALGRSWQCSTIQIDFNNPERFELSYIGEDGKSHQP
                     IMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVMAITDNQREFVTEVTAHMKAS
                     GFRVESDIRNEKIGFKIREAEKAKIPFMFVVGDREVQSRSLSVRGRSGANLGTMTVQG
                     ALDLLKTEVERAGQQLSPTS"
BASE COUNT          695 a          992 c          942 g          838 t
ORIGIN      
        1 ggcagggcag attgtggtat cgggaaagcc gctgggtatc atcggagaag ttcaccccga
       61 agtgcttgaa cggtggcaaa tcgccgtacc ggtcgccgcc ttcgacgtca acctctctcg
      121 actcgttgag atccattgag cggaggagga cgctcatccc cctccaacgg tggagtttcg
      181 aaaaggtgga gttatgcggt cgccggtgtc aaatgttgtt tggccaggcc gacaagctgc
      241 tcgaatcctg cgacatcctt gatcgccata tcggacagca ccttgcgatc caacaatacg
      301 ttggcttttt taagcgcatt gatgaagcgg ccgtacgtga ggccgtgcgc ccgcgtggcc
      361 gcgctgatgc gagcgatcca caactgccgg aagtcccgct tccgattctt gcgcccggtg
      421 taggcatact gctgtccctt atctaccgat tctgtcgctg agcgaaacag ccggctcttt
      481 gcgccatatt gccctttcgc cagcttgagt cgttttttcc gtcttgctcg tgtttttggc
      541 ccaccttttg cgcgtggcat gactcatact ccttgttgag ctcaggttaa catctctatg
      601 tatagggcag taggcgcttg agtgccgcgg ccttggtttc gtcaacgacc accgatccgc
      661 tcagccggcg tttccgctca cgattcttgc tggtcagaat atggcgcttg ccggcgcgtt
      721 tgcgcatgaa tttgcccgtg cctgttctgt gaaaccgttt gcttgctccg ctatgtgact
      781 tcatcttcat gttcgcatcc tttggttaag cgttcggcct ctctaattct taggagcgac
      841 gatcatgatc aagctgcgcc cttccatccg cggcgcatac tcgatcgtac cggattcgga
      901 tagctcagag atcactgagt tcatgacgac ccgtcccatt tcctgattgg ccatttctcg
      961 cccccgatac gtcaggacga ccttcgtctt gttgccctcc tccaggaagc ctttgatctg
     1021 tcggatctta atctccaggt catgcttatc ggtgcgagga cgcaacttga tttccttcac
     1081 ctgagttgat ttttggtggc gacggctttg gtgatctttt ttgctcagct cgtacttata
     1141 cttcccgtaa tccatgatgc ggcagaccgg tgggaccgac gtcggggcca cttccaccag
     1201 gtcatatccg ccttcttgtg cctgcttgaa agcttcggga gtcggaagaa tgccgagttg
     1261 ctcaccttcc ggcccaatca ctctgacttc acggactcga atttcgcggt tcacccgcag
     1321 tttggggacg ataagccacc tctcatgatg taggggaaag ttgctgcccg gcccgctcga
     1381 cttcagtctt gagcagatcc aacgcgcctt ggacggtcat tgtgccgagg ttagcgccgc
     1441 tacgacctct cacggacagt gagcgactct gcacttcgcg gtcgccgacg acgaacataa
     1501 acggtatctt cgccttttcc gcttcacgaa tcttgaatcc gatcttttcg ttccggatgt
     1561 cggactcgac gcgaaagcct gaggctttca tgtgtgcggt cacctccgtg acgaattcac
     1621 gctgattgtc tgtaatcgcc atgaccaccg cttgcaccgg tgccagccat gttggaaagg
     1681 cgccggcata gtgctcgatg agaatgccga aaaatcgctc gatggacccc atgagcgcgc
     1741 ggtgaatcat gataggttga tgactctttc cgtcttcgcc gatgtaactg agttcgaacc
     1801 gttccggatt gttgaagtca atctggatgg ttgagcattg ccaggatcgg ccgagcgcat
     1861 ccttgatctt gatgtcgatt ttcgggccgt agaatgcgcc tccgccgggg tcaagatgaa
     1921 acgagatgtt gcggcctttc aacgcggctt cgagtgcgct ggtcgcgagc gtccaatgct
     1981 cctctgcgcc gactgattcg ttcggacgag ttgaaagaaa cacttcgaat tccatgaacc
     2041 caaaggtgcg caaaatgaag aaggtgaagt ccagcacgcg gctgacttca gcttctattt
     2101 gatcaggacg gcaaaacaaa tgcgcgtcat cctgagtgaa gccacgcacg cgaagcaggc
     2161 catgaagcac gccggtgcgc tcgtatcggt acaccgtccc caattcgcca tagcggattg
     2221 ggagctctcg gtagctccgt aaatgtgatt tgtaaatcat gatgtgatag gggcagttca
     2281 tcggcttgag ttgatactcg ctgccctcga gcttcatcgg cgcaaacatg ttgtcgcgat
     2341 agtagtcgac atgtccgcgg gtcttccaca gatccaaccg cgccacgtgg ggcgaataca
     2401 ccagctcata gccgtcccgg agatgctgct cgcgccaaaa attctcgatc agcagtcgaa
     2461 tgacggcacc cttcggatgc cacagcacca acccgggtcc gatctcgtcc tggatcgaaa
     2521 ttaggtctaa ttctttcccc actttgcgat gatcgcggcg tttgatttct tccagcttgg
     2581 ccaggtgagc atccagctcg gccttcgtag gaaaagaggt gccgtaaatt ctctggagca
     2641 ttgggttgcg ctcatcgccc cgccagtagg cgcctgccgt cgtcaaaagc ttcagggcgc
     2701 ccacgtgacc ggtcgacggc acatgtgggc cacggcaaag gtccacgaac tcaccctgcg
     2761 tataaatgga aatcggctct tgatccggaa agccttcgat caactccacc ttgtaatgct
     2821 ccccgcgaga tctaaagagc tccaatgcct cttgcttcgt gaggtcgcgg cgggtgacgg
     2881 caagcttgcg tttcgcgatt tcgcgcgccc gttgctcgat gttctcgaga tcttcaggcg
     2941 tgaagggccg atcgtaggca aaatcataat aaaaactgtc ttccaaagcc ggcccgatcg
     3001 tcagttgagc ggagggaaaa agttctttca cggcttgcgc catgatgtgt gtgctgctat
     3061 gccggtagat ttcccggcct tcggcagtgt cgaatccaag tggatcgatg gtcgaatctg
     3121 cggtcaaagg cgcagacaaa tcggtcggcc tcccgttgac cgaggcggcc agcacctcgg
     3181 gcccgacagg aattccgcca gcggccaggg cttgggcaat agtataacca ggagggacgt
     3241 caaaactctt cccattcttc agggtgactt tcggcacatt cctctctcta tggctcgtag
     3301 gcgcagacca ccttcccttg agaatccggt tgaacgccga aatctcacga ggcgggaaac
     3361 atcctgtttc tcagcaggat acaaaaatac aaaaggcacc ccaaccgcag caggcggcca
     3421 gatgccctcg gcgagatggt aggcgcggac ggtcttgaac cgtcgac
//
LOCUS       DASUTG010000460         6592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1552765, whole genome shotgun sequence.
ACCESSION   DASUTG010000460 DASUTG010000000
VERSION     DASUTG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552765"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF089_10515"
     CDS             <1..474
                     /locus_tag="VF089_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX6174439.1"
                     /translation="GKGDELKTAGYSLNPNYRYPQGGKPEITGYTAANVLQIRTGHLA
                     GVGKLIDASMQAGANQINRLVFTLKDEHSAQVQALRMAAQKAQSKAEEMAGSLGLKVV
                     RVLSLTENERGVRPIIAQTRSVQAEALSSAAPTPVEAGTIEVRSSVTLTAEIAPR"
     gene            complement(607..2052)
                     /locus_tag="VF089_10520"
     CDS             complement(607..2052)
                     /locus_tag="VF089_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6174440.1"
                     /translation="MTDGHAKKFAMEDIERNSESIALLCDNIFYFAELGMQEFETSGL
                     MMQVLETAGFTIERNPSGMPTGFIASYGSGKPVIALHTEFDATPGCSQASGVAEPTPI
                     VEGAPGHTEGHNVNGAVMIGAALAIKKTIDEFGLKGTLKVFGAPAEEQLVSRPYFVRD
                     GYFKDVDVALHDHIGKELGTVYGLRQYALISAEFTFKGQSAHAATAPWNARDALDAAV
                     LMDIGWDKLREHLEPTQRSHRVITNGGDQPNVIPNRSTIWWFFREATAEKTRELFEKA
                     KRVAQGAAMMTETSYDVNVLSAVWPTRANRTLAEVIQRNIEEIGMPQWTEGEQKLVHE
                     VQIKVKAKPTGMPTKVAPLKECVQSVSANDSGEVSWVVPTGLINFPSNIPGVSYHHWS
                     AGVSLATSIAHKGAVAGAKAMAASAIDLLLDPQIVAQAKETFKQEIGDVEYRPLLPPD
                     QKPPLELNRDLMERYRPKMREHYLKDRPEFR"
     gene            2505..3059
                     /locus_tag="VF089_10525"
     CDS             2505..3059
                     /locus_tag="VF089_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6174441.1"
                     /translation="MNKDITRESGDMADVVERNIQALLSRRNQEEKTKGVQERIADAI
                     SRFAGSMIFVYLHLIGFGVWIIVNLGWTPLPTFDPTFVVLAMFASVEAIFLSTFVLIT
                     QNRMAAQADRRADLDLHISLLAEHEVTRLISLVNAIAERMGIEPASDHPELAELEKDV
                     DPEKVLEKIEESESNMTKKAPDQA"
     gene            complement(3121..3999)
                     /locus_tag="VF089_10530"
     CDS             complement(3121..3999)
                     /locus_tag="VF089_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6174442.1"
                     /translation="MKNPRYDYSPIIRREKIAWPDNGRIALWVAPNIEYFHFDMPIRG
                     SGSNHKPDVPGYTLRDYGSRVGVFRIMSVLDKFGIRASVLLNAEVCEHHPEIIAEGNK
                     RNWEWLGHGLTNSVSMLDYSADEERGIIHKVKEIITAATGKEPKGWLGPGLGETFDTP
                     DHLAAEGFEYVCDWGNDEQPTPMRVRTGRMIVVPYELGVNDIRVFNRENHTAEQYYRM
                     VCDHFDTLYRDSISGGRVLCLPLHPFVIGLPFRISYLEKALEYICSHEGVWRTTSGEI
                     AQFYYQNYYKDPGRLT"
     gene            4305..4580
                     /locus_tag="VF089_10535"
     CDS             4305..4580
                     /locus_tag="VF089_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX6174443.1"
                     /translation="MADLTEEQRRSYWRYNIRLTTILLIIWFVVTYLVSGLWAGWLNQ
                     FSFLGFPLGYYMAAQGSLAIFVIEIAVYAYLMNKLDREYGIDEGGAA"
     gene            4577..6505
                     /locus_tag="VF089_10540"
     CDS             4577..6505
                     /locus_tag="VF089_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX6174444.1"
                     /translation="MSLGKIFGLYTLSFLFVTILIGIAEQIFGLPPRWIGWIFMGLSI
                     GIYIVIGIITRTSNPDQYYVAGRGVPAVFNGMATGSDWMSAASFISMAGALSAQGFAG
                     LAYVMGWTGGYLLLAVFLGPYLRQFGAYTIPDFLGARYGGNFARVIGVVAAIACSFTY
                     LIAQVTGVGLIVSRFIGLDFNIGVFVGLIGVLFCSVLGGMRSVTWTQVAQYIILIISY
                     LVPVVYLSYQLFAIPIPELTYGRLLQTNNAKAIEITRDAREKETRALWAKEAEQANAK
                     LKAGDVDEATAEKLRAAAKIATQQATAPAASDDATLGRYLTVPSGVGMWNFLALTFCL
                     MVGTAGLPHILTRYYTTPSVRQARTSVAWSLFFIFLLYFTAPAYAAFARFAIYARLVG
                     TQIAELPRWITLWQPAGLFEVIDKNGDGVVQWADFVVKSTDFVVLSMPEVAGLPFVIT
                     GLVMAGGLAAALSTADGLLLTIANAFSHDLYYNVIDPKASLTKRLTITKILLIVTALV
                     AAWVATFRVAIIVELVAWAFSLAGASFFPALVMGIWWRRANKAGACAGMIAGLAVTAY
                     YMIGSRFYGVSWFGTQTIASAVFGLPVGFLTIWIVSLLTEAPPKPVQDLVASVRYPKS
                     GQAITSDPLGRGVPIGAR"
BASE COUNT         1391 a         1886 c         1735 g         1580 t
ORIGIN      
        1 ggcaaaggcg acgagctcaa aaccgccggc tactcgttga atcccaacta ccgctatccc
       61 caaggcggca agccggaaat aacgggctat acagcggcca atgttttgca gatcagaacc
      121 ggacacttgg caggggtcgg caagctaatc gacgccagca tgcaagcggg cgctaatcag
      181 atcaaccggc ttgttttcac attgaaagat gagcattcgg cgcaagttca agcgctgcgc
      241 atggcggcgc aaaaggcgca gtccaaggcc gaagaaatgg ccggctcctt agggttgaaa
      301 gtcgtcaggg tcctatctct aactgagaat gaacgaggag ttcgcccgat catagcgcag
      361 acccgcagcg tgcaagcaga agcactttca tcggccgcac cgacaccggt cgaggccggc
      421 accatcgagg tgcgctcctc cgtgacactg accgccgaaa tcgctccgcg ctaagcgctg
      481 gcatatttcg gaacagcagt acgcagtctt tccctgaaac gatttgaact ttttgaacga
      541 cttgaacggc ttaaaccgct tggccttcag ctctgacctt gaaccgtgga acggttgaac
      601 aattctctat cgaaactccg gcctgtcttt tagataatgc tcacgcatct tcggccggta
      661 cctttccatc aaatcacgat tcagttccag cggcggcttc tggtcaggcg gcagtaaagg
      721 ccggtactcg acgtcaccga tttcctgctt gaaagtttcc ttggcctgcg caacgatctg
      781 cggatccaac aacaaatcaa ttgccgatgc cgccatcgct tttgctccgg ccaccgcacc
      841 tttgtgcgca atcgacgtgg ccagagacac gccggcggac cagtgatgat acgatacgcc
      901 gggaatattc gagggaaagt tgatgagccc ggtcggcacc acccacgaga cctcgcccga
      961 atcattggcg gagactgatt gaacacattc tttgagcggc gcgaccttcg tgggcatgcc
     1021 cgtcggctta gccttcactt tgatctgaac ctcgtgaacc aacttctgct cgccttccgt
     1081 ccactgcggc atgccgatct cttcgatgtt tcgttgaatg acctccgcca gcgttcggtt
     1141 ggcccgggtc ggccagactg cgctgagaac attcacatcg tacgaggtct cggtcatcat
     1201 cgccgcgcct tgggcaacgc gcttagcctt ttcaaacagc tcccgcgttt tctcggccgt
     1261 ggcttcacgg aaaaaccacc agatcgtcga ccgatttgga atgacattgg gctggtcgcc
     1321 cccgttggtg atcacccgat ggctgcgctg ggtcggctcc agatgctccc gcagcttatc
     1381 ccaaccgata tccatcagca ccgccgcatc caaagcatcg cgggcgttcc acggggccgt
     1441 ggcggcatga gcgctctgtc ctttgaaagt gaactccgcg gaaatgagcg catactgacg
     1501 cagaccgtag acggtgccaa gctctttgcc gatatgatca tgcaatgcca catcgacatc
     1561 tttgaaatag ccgtcacgga caaaatacgg tcggctgact agctgctcct ccgccggagc
     1621 gccgaaaacc ttcagtgtgc ccttgaggcc gaactcatcg atggtctttt tgatcgccaa
     1681 tgccgcgccg atcataacgg cgccattaac gttgtgtccc tcggtatgcc ccggagcgcc
     1741 ctcgacaatc ggcgtcggct ccgccacacc ggaggcctgc gagcatcccg gtgtcgcatc
     1801 aaattccgtg tgcagcgcga tcaccggctt acccgacccg tagctcgcta tgaaccccgt
     1861 cggcattccg gatggattgc gttcaatggt aaatcccgct gtttccagaa cctgcatcat
     1921 caagccggag gtctcgaact cctgcatgcc gagctcggcg aagtaaaaga tgttgtcaca
     1981 gagcagcgca atcgattcac tgttgcgctc gatgtcttcc atggcaaatt tctttgcgtg
     2041 accatcagtc atattcggtc tcctgttcaa aaaagatgct ttgttttcga tatcacgaag
     2101 cgatgaacaa cttacagccg actcttactc tactttagta tctcgacgat tttcacgttc
     2161 gcaagctcag gtcgttgctg ttatcggact aacgtagagt atcctcctct ccagccgctc
     2221 tccccgccaa gatgtcccgg ctacgaggca attccaagtt atatttctta accctgaaac
     2281 aaacgactat ccattcatac gtgattgtga gcggtgtctc agctcatagg atcaacccct
     2341 gtatatgatt ccggcgagcg gcacacggct tgctcaacca actgcgactc cagccaatct
     2401 ctgagagaac ccgaatcgct gctccccata gtaaatgaca ggagttagaa ttcgcaataa
     2461 cactagattc aaagcgcagt aaacgaacca acaaagttaa aatcatgaac aaggatataa
     2521 cccgcgaatc tggcgatatg gcagatgtcg ttgagagaaa cattcaagcg ttactatccc
     2581 ggcgcaacca ggaggaaaaa acaaaaggcg ttcaagagcg aatagcggat gcaataagcc
     2641 gcttcgccgg cagcatgatt tttgtctacc tacacttaat cggcttcggc gtttggatca
     2701 tcgtgaatct tggatggaca ccgctgccaa cctttgaccc cacctttgtg gttttggcaa
     2761 tgttcgcatc cgtcgaagcg atttttcttt cgaccttcgt actgattacg caaaatcgaa
     2821 tggctgcaca ggccgaccgt cgcgccgatc tcgatttgca catcagtctt ttggctgaac
     2881 acgaagtcac ccgcttgatc agcctcgtca acgccatagc cgagcgtatg ggaatagaac
     2941 cggcgtccga ccacccggag ttggcggaat tggagaaaga tgttgatcca gaaaaggtcc
     3001 tggagaaaat cgaagagtca gagtcaaaca tgaccaagaa agctcctgat caagcttgag
     3061 catggttgcg gatccatccg aaaagtccgc ccatcaggtc gcatgccggg gctgcaaata
     3121 ctaagtcaat cttcccggat ctttgtaata gttctgataa taaaactggg cgatttctcc
     3181 actggttgtg cgccagactc cctcatgaga gcaaatgtat tcgagcgcct tttccagata
     3241 tgagatccga aacggcaggc cgatgacaaa cggatgcagc ggcaggcaaa gtacccggcc
     3301 accggaaata ctgtcgcggt aaagcgtgtc gaagtgatcg cacaccatgc ggtagtattg
     3361 ttcggccgtg tggttctcgc ggttaaagac ccggatgtca ttcaccccga gttcgtaagg
     3421 cacgacgatc attcggcctg tcctcacccg catcggcgtg ggctgctcgt cgttacccca
     3481 atcgcaaacg tactcgaatc cttcagcggc gagatgatcc ggcgtgtcga atgtctcgcc
     3541 cagtccggga cctaaccagc ctttcggctc tttgccggtg gctgcagtga taatctcctt
     3601 aaccttatgg atgattccac gctcttcatc agccgagtaa tccaacatcg acacgctgtt
     3661 ggtcaaaccg tgtccgagcc attcccaatt acgtttattg ccttctgcaa taatctccgg
     3721 atggtgttcg cagacttcag cattgagcag aacgctggcg cgaattccga atttatcgag
     3781 cacactcatg atccggaata cgcccacccg tgatccatag tcgcggagag tatatcccgg
     3841 cacgtccggc ttgtgattgc tgcccgatcc acggatcggc atatcgaaat gaaagtattc
     3901 gatattaggc gccacccaga gggcgatgcg gccgttgtcg ggccaggcga ttttttctcg
     3961 acgaataatg ggactgtaat cgtaacgcgg atttttcatt tggatctctt acctccttgg
     4021 tgaagaactg attagtctgg ttagaaatta agctgcatca ttcatggata cttgtaggtt
     4081 cttttataaa tattcatttg acacatcgtt gcagcctttg ctacaagccg tgcagagccc
     4141 gttcactgcg tttcgcatgg ccatctccaa gggctttccg cagtgaagat ttccctccca
     4201 aagtgtgata cccggtgcgc gggcgcactt tgacgtgctt acatcgaccg gtatgacgag
     4261 tttagcttca agggatacga acgattagca tcgaggaggt gctcatggct gatctcacag
     4321 aggagcaacg aaggtcgtat tggcgctaca acatcagact caccaccatt ctcctgatca
     4381 tttggtttgt cgtaacctac ctcgtttccg gactgtgggc ggggtggctc aatcaattct
     4441 cgttcttggg atttccactc ggctactaca tggcggcaca gggctcgttg gcgatcttcg
     4501 tcatcgagat cgccgtgtat gcctatctga tgaacaaact cgatagggaa tacggcattg
     4561 acgaaggagg tgcggcatga gccttggcaa aatcttcggt ctatatacat tgtcgtttct
     4621 cttcgttacg attttgatcg gcatcgccga gcaaatcttt ggcctgccgc ctcgatggat
     4681 cggctggatc ttcatgggtc tatcgatcgg catttatatc gtcattggta tcatcacccg
     4741 cacctctaat cccgatcagt attacgtcgc cggacggggc gttccagccg tgttcaacgg
     4801 tatggccacg ggttccgatt ggatgtcggc cgcctcgttt atctcgatgg ccggggcgct
     4861 ttcggcgcaa ggcttcgctg gtctcgccta cgtcatgggt tggaccggcg gttatctgtt
     4921 gctggccgtc tttctcggtc cttacctgcg ccagttcggc gcctatacca ttccggattt
     4981 tcttggcgcg cgctacggcg gcaatttcgc cagagtcatc ggcgtggtcg ccgccatcgc
     5041 ctgctcgttc acctacctga ttgcccaggt gactggcgtc ggcctcattg tctcgcggtt
     5101 tatcggcttg gatttcaata tcggcgtctt tgtcggactg atcggcgtct tgttctgttc
     5161 ggttttaggc ggcatgaggt cggttacttg gacacaggtc gcgcagtaca tcattctgat
     5221 catttcctat ctggtaccgg tcgtctatct gtcgtaccag ctttttgcca ttccgatacc
     5281 tgagctgact tacggccgct tgctccagac caataatgcc aaggccatcg agatcacccg
     5341 cgatgccagg gagaaggaga cacgcgccct gtgggcaaaa gaggcggagc aggccaatgc
     5401 caagctcaag gcgggcgacg ttgatgaggc aactgcggag aaactcaggg cagctgcaaa
     5461 aattgctacg cagcaagcga cggcccctgc cgccagtgac gacgccacac tcggacgtta
     5521 tctcacggta ccgagcggcg tcggcatgtg gaattttctg gcgttgacct tctgtctaat
     5581 ggtaggcacc gccggcctgc cccatattct cacccggtat tacaccaccc catcggtgcg
     5641 gcaggcgcgt acatcggtgg cctggtcgtt gttttttatt ttcctgctct atttcaccgc
     5701 gccggcctat gcggctttcg cccgatttgc tatttacgcg cgcctggtcg gaacgcagat
     5761 tgccgaactg ccgcgctgga ttacgctttg gcaacccgcc ggtctcttcg aggtcatcga
     5821 caaaaacggc gacggcgtcg ttcagtgggc agacttcgtg gttaaatcca ccgacttcgt
     5881 cgtcttgtcg atgccggagg tcgccgggtt gccgtttgtc atcaccggct tggtgatggc
     5941 cggaggcttg gcggcggcgc tgtctaccgc ggacggcttg ctgctgacca tagccaacgc
     6001 cttttcacac gatctctact ataacgtcat cgatcccaaa gcctcgctga ccaaacgact
     6061 cacaattaca aagatcttgc tgattgtaac cgcgctggtc gccgcgtggg tggcgacctt
     6121 ccgtgtggcg atcatcgtcg agctggtcgc ctgggctttc agcttggctg gcgcctcctt
     6181 ctttcccgct ctggtcatgg gcatctggtg gcggcgcgcc aataaggccg gggcttgcgc
     6241 cggcatgatc gccggtcttg cggtgaccgc gtattatatg atcggcagcc gcttctacgg
     6301 cgtttcatgg tttggcaccc agactattgc ctcggcggtt ttcggactgc cggtgggttt
     6361 cctgacgatc tggatcgtgt cgctgttaac cgaggcgccg cccaagcctg ttcaggatct
     6421 ggtagcgagc gttcgttacc ccaagtccgg ccaggctata acctccgatc cattgggtcg
     6481 cggggtaccg atcggcgcgc gctagaacga tgtccacgcg agggacgagg agacctcgcg
     6541 tggacttttt atgaacagat tcgtcttgca gctggtcgtc cttctgcgct tt
//
LOCUS       DASUTG010000461         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_515144, whole genome shotgun sequence.
ACCESSION   DASUTG010000461 DASUTG010000000
VERSION     DASUTG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515144"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF089_10545"
     CDS             complement(<1..442)
                     /locus_tag="VF089_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174445.1"
                     /translation="MFVGAPARRSRRVKVILLKKDSTIQRRQKLAPPKPLSGKERRRI
                     NPNHGLVKLFERNMDRLISRFLYPHLSAIWHPYGWVIRRRFRVAETTLSPAGWPQGLP
                     SLRVLLLSDIHCGIFLKPETLADVVKFLMQLQPDLVVVTGDIVTG"
     gene            670..>2158
                     /locus_tag="VF089_10550"
     CDS             670..>2158
                     /locus_tag="VF089_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174446.1"
                     /translation="MTIVKQPIVFLIALMLFRWPALGQAAESASAQQPRSGGELVFAV
                     GELPPSFDGHRETTFGMLHPVAPHYSTLLRFDPQNYPKIVGDIAERWSFSRDGLTATF
                     TLRKGIKFHDGTPLTARDVQASYDKIIFPPEGVASARKASFAMVDKIEAPDDATVIFR
                     LKHIAASFLANLASPWNFIYSAERLKKDPRWYEKNILGSGPFIFGEHVAGSHWTGKRN
                     PNYFMAGRPYLDGFRAVFIRDTAPRVAAVRSGQVLAEFRGFNPAARDDIVKALGNKAV
                     VQESPWACNILVAVNNEKKPFNDVRVRRALNLAIDRNEGSKALSQISVMKYVGGVFRP
                     GSEFATPPAELTKLAGFGKDIESSRKEARRLLKEAAVPEGFSFVLKNRNVKEPYEVSG
                     VFLVDQWRKIGLNVTHSPQEGGPYFNDLRQGNFDAGVDFACDFMDEPDLYLLKYVSTE
                     KSPINYSRFKDPTFDELYERQSRMLDLKDRLPLLRQMERRAIAEQA"
BASE COUNT          496 a          582 c          604 g          476 t
ORIGIN      
        1 ggccggtgac gatatcaccc gtcaccacca ccagatctgg ttggagttgc atgaggaact
       61 tgacgacatc ggccaaggtc tcgggcttta gaaaaatgcc gcagtgaata tccgatagca
      121 agagcacgcg caacgatgga agcccctggg gccagccagc gggcgacagg gtcgtctccg
      181 ctactcggaa acgccgccgg atcacccagc cgtacgggtg ccaaatggcg gagagatgag
      241 ggtacagaaa acgcgatatg agccgatcca tattgcgttc gaaaagcttg accagcccat
      301 gattcggatt gatccggcgg cgttccttgc cgctgagcgg cttgggtggc gcaagttttt
      361 gtcgtctctg tatggtggaa tctttcttca ggaggatgac tttgactcgt cgggagcgtc
      421 tcgctggcgc gcccacaaac aaaaactatt ttgtcctaaa ttattgcagg agacaatgga
      481 tatactgctc cgctctgttt cgtgcgacca accgattaga cgagccatca aagactttcg
      541 attgcgcagg aacgcacgct ctctacagct tgacatcacg aagtcgaatc acctattttc
      601 gcgcagccac atgtttgtac tctgaaacct aaggtgatcg attgtccagc aactgaagag
      661 agggccttta tgacgatagt taagcaaccc atcgtgttcc tgattgctct aatgttattc
      721 cgatggcctg cgttaggcca agcggccgag agtgcctccg cgcaacagcc gcgctcggga
      781 ggagaactcg tcttcgccgt cggcgaactc cccccgtcgt tcgacgggca tcgtgaaaca
      841 actttcggca tgctacaccc ggtagccccg cattacagca cgctgctccg gttcgatccg
      901 cagaactacc cgaagatcgt tggcgatatt gccgaacgct ggtcgttctc cagagacggc
      961 ctgacggcca cattcactct acgcaaaggc atcaaatttc acgacgggac tccactgacg
     1021 gcgcgcgacg tccaggccag ttatgacaag atcatttttc ctcccgaagg agttgccagc
     1081 gctcgcaaag cttcctttgc catggtcgat aagatcgaag cgccggatga tgcgaccgtg
     1141 atttttcgtc tcaaacacat cgccgcatct tttttggcaa atctagcgtc cccgtggaat
     1201 tttatttata gcgcggagcg actcaagaaa gacccgcgct ggtacgagaa aaacattttg
     1261 ggctcggggc cgtttatctt cggcgagcac gtcgccggtt cgcattggac cggcaaacgg
     1321 aatcccaatt actttatggc cggccggccc tatctggatg gattccgggc cgttttcatc
     1381 cgcgataccg ctcctcgtgt ggcggcggta aggagcggtc aagtgctcgc cgaatttcgt
     1441 gggttcaatc ccgccgcccg ggatgacatt gtgaaagccc tgggaaataa ggcggtggtt
     1501 caggagagcc cctgggcctg caatatctta gttgctgtta acaacgagaa aaagccgttt
     1561 aacgatgtgc gagtcagacg ggcgttaaac ctcgccatcg atcgcaacga ggggtcgaag
     1621 gcgttgtcac aaatttcggt gatgaagtat gtaggcggcg tctttcgacc gggctccgaa
     1681 ttcgcgacac ccccagcgga actgacgaag cttgccggat tcggtaagga catcgaatca
     1741 tcgcgcaaag aagcgcggcg actgttgaag gaagcggcag tgccggaagg attctcgttt
     1801 gtcttgaaga accgcaatgt caaggagccc tacgaagtga gcggtgtgtt tctcgtcgat
     1861 caatggcgaa aaatcggcct caacgtgacg cactcgccgc aagagggtgg cccctacttt
     1921 aatgacctgc gccaaggaaa cttcgatgcc ggcgtggatt tcgcttgtga cttcatggac
     1981 gaacccgatc tttacttgct gaagtatgtg agcactgaga agagcccgat caattactcg
     2041 cgttttaagg accctacgtt tgacgagctt tatgaacggc agagccggat gctcgatttg
     2101 aaagatcggt tgccgttgct acgccagatg gagagacgcg caatcgccga acaggcct
//
LOCUS       DASUTG010000462         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1997906, whole genome shotgun sequence.
ACCESSION   DASUTG010000462 DASUTG010000000
VERSION     DASUTG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997906"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..1507
                     /locus_tag="VF089_10555"
     CDS             497..1507
                     /locus_tag="VF089_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174447.1"
                     /translation="MSKHRAKLHMLIAVCVCLFLLCTNALTSDAAAPPNPLMITFGSL
                     SERETALFVARDYGIFAKHGLDVKLVHVRNGAVALSALASGDAQFYLGAATGSTLGAI
                     AGGLDAVFVTSLIHKLEGTFVVAPAIKSPTDLRGKTVGVTSIGGGVWMRSMLTFEHWG
                     LEPQRDKINLRVVGDESLLAQAMPNGIIDGSYFNYTFASVLERQGFRVLADLAKLNIP
                     YQNTALLAQRRYLNTAPETTERLLRAVSDAVIFVLEPKNKELVLKSLSKGLRLPRPED
                     ALGGYDRLANLYERRIFPNVDGIRSTIRFLGPGNEKIRALKAENLIDDRFVKKLEQEG
                     RF"
     gene            1868..>2734
                     /locus_tag="VF089_10560"
     CDS             1868..>2734
                     /locus_tag="VF089_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6174448.1"
                     /translation="MKNPRIIIAGGGPVGVVSALACAHYGYAVTLLEAEDKIDDNPRA
                     ATTHPSTLELIARVGLIDQFIREGLVARFFQFWDKPSRTKIVEFDHDILRDETPYPFV
                     VQTEQHKLARMGIERLRTSPDAQVRFSSRATTVSQDADGATVTIEGAEGTETLRGDYV
                     IGADGGRSTIRKALEIEFEGFTWPERFLVLTTIDDFQKLLTGCCYRNYLADPEEWTNL
                     FKVAGDDGKGRWRAVFPTRVEESDEEALGDESTYSRLQRVFPMDREYNVVHRNLYKVH
                     QRVAATFRKGRVF"
BASE COUNT          613 a          710 c          774 g          637 t
ORIGIN      
        1 ggatattcgc caggtttatt ccttcgcgta aaaacaccca gtgcgctttt acgcgggcgt
       61 ctgtgaacct tacactgcga agttcacaga agaattgagg tacatcggcg gcagaatcgc
      121 cgtcgccgcg gaactcgggc aatcaaaggt gtgaaaggcg tcccgggctg cgtctggccg
      181 tgtccctcgt tgtcgttgaa aatcgattct tcggtattga tgccttaaga tatttcttcc
      241 agagtgcctt ataacgaacg atctccaatc agccagcgtg agcatcccct caggcggcat
      301 ttagcaatta aacttcttta aggctttgcc atccatcgat acggcgcatc gtggtatttc
      361 actcggcgaa acattcgtca ggaagtacga agtgaccgac cgtgagagcg aactccacta
      421 tagaagcccc gctcagatcg ggatataacc gccgctgagg aaaagatatc aaaagtaagg
      481 gtttcgtgga ggatttatgt cgaagcaccg tgcgaaactg catatgctaa tcgccgtttg
      541 cgtttgttta tttctactgt gtacaaatgc gctcacttcg gatgccgcgg cgcccccgaa
      601 tccgctgatg attacgtttg gcagcctcag cgaaagggaa actgcgctct tcgttgctcg
      661 agattatgga atctttgcca agcatggtct ggacgtgaaa ttggttcacg tgcgcaacgg
      721 cgccgtcgcg ctttcggctt tggctagcgg agatgctcaa ttctatcttg gagctgccac
      781 cggttctact ctgggtgcca ttgccggcgg tttggatgcg gtgttcgtca ccagcttgat
      841 acacaagttg gaaggcacgt tcgtcgtcgc tccggctatc aaatccccga cggacttaag
      901 aggcaaaacc gtcggtgtca caagcatcgg cggcggcgtc tggatgcgct cgatgctgac
      961 tttcgagcat tggggcttgg agccgcagag ggataaaatc aatttgcggg tggtggggga
     1021 cgaatccctg ctggctcagg cgatgccgaa cggcatcatt gacggctctt atttcaacta
     1081 cacctttgct tccgtcctcg agcgccaggg gtttcgtgtc ctcgccgatc tcgccaagct
     1141 caatatccct tatcagaaca cagcgctgtt ggcgcagcgg cgatatctta acacggctcc
     1201 tgagacgacc gagaggttat tgagagctgt gtccgatgcc gtgatcttcg tattggagcc
     1261 caaaaacaaa gagttggttc tcaagagcct gtccaaggga ctgcgtttgc cgcgtccgga
     1321 ggacgcgctc ggcggttacg accgcctcgc aaatttgtac gagcgccgca tctttcccaa
     1381 cgtggatgga attcgttcga cgatccggtt cctcggccct ggcaatgaaa agatccgcgc
     1441 tctgaaagcg gaaaacttaa tcgacgatcg attcgtcaag aagttggaac aggaaggccg
     1501 tttttgagtt ggtctaccga aattgacgga cttgctaatg ccacaatgag cgatggcgac
     1561 gcgttgaacg actcggcagg caccgagaat cgtgctaacg tagctgccaa atgcgtatag
     1621 cagacttgta gtgcttatcg aagcgctgaa tgaatcctgg aattacgccg gaatgtctag
     1681 caaataaaag atcgagctac cgcgagacaa gattgcggag ttctgcaaaa gtaaccgaat
     1741 acgaaaattg tcgctctttg gttcggcgtt gagagatgag cttcggaacg atagcgacgt
     1801 tgatctttgg ttgagttccg tcctgtatcg ggatcgatta gcactgagct tcggagattt
     1861 ctttacaatg aaaaaccccc gtatcatcat cgccggcggt ggtccggtcg gcgtcgtgtc
     1921 ggcgctggcc tgcgcacatt atggctatgc ggtaacgctc ctcgaagccg aggacaagat
     1981 cgacgataac ccgcgagcgg caaccaccca tccctcgact ctcgagctga tcgcccgcgt
     2041 tggcctgatc gatcaattca ttcgagaagg cttggtggcg cggttcttcc agttttggga
     2101 taagccgtcg cgcactaaaa tcgtcgagtt cgatcacgac atcctgcgtg atgagacgcc
     2161 ctatccgttt gtcgttcaga ccgagcaaca caagcttgcc aggatgggca tcgaacggct
     2221 ccggacttct cctgatgccc aggttcgctt ctccagccgc gccacaactg tcagtcaaga
     2281 tgccgatggc gcgacggtga ccattgaggg agcagaggga acggaaaccc tgcgcggcga
     2341 ttatgtcatc ggcgccgatg gcggccgcag caccattcgt aaggctctgg agatcgaatt
     2401 cgaagggttt acctggcccg agcgctttct cgtgctgacc accatcgacg actttcaaaa
     2461 attgttaacg ggctgctgct atcgtaatta tctggcggat cccgaagagt ggaccaattt
     2521 gttcaaagtg gcgggcgacg acggcaaggg ccgttggcgc gcggtgtttc ccacacgggt
     2581 ggaagagagt gacgaagagg cgctgggaga cgagtcgacg tattcgcggc tgcaacgcgt
     2641 attcccgatg gaccgagaat ataatgtcgt tcatcgcaat ctgtataaag ttcaccaacg
     2701 agttgccgcc acattccgca aggggcgggt gttt
//
LOCUS       DASUTG010000463         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1553345, whole genome shotgun sequence.
ACCESSION   DASUTG010000463 DASUTG010000000
VERSION     DASUTG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553345"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..896)
                     /locus_tag="VF089_10565"
     CDS             complement(75..896)
                     /locus_tag="VF089_10565"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174449.1"
                     /translation="MKLFTTGEVAKILNLPGSRIRSFVRAGFLKPGRGKKKSLQFTFQ
                     DVLFLKTAKGLLDSRVPPKNIIRMLLSLKRQLPGDRHLSSLKIYADNRRVIAWDGKAR
                     WQPDSGQFLFNFDARAVVQKLNAPITPAKPQASELSAQHWFDLALELEESSIKEAQRA
                     YHMALERDSKMADAHLNLGKLYHDQGELKKAEAHYRAAADSAPMDPAPRFNLGVLLED
                     ARQIQAARRAYEQAVELDPDFADAHYNLALLCESLNEKVEALAHFRAARKLYLKK"
     gene            complement(893..1750)
                     /locus_tag="VF089_10570"
     CDS             complement(893..1750)
                     /locus_tag="VF089_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6174450.1"
                     /translation="MPPHSLGSGTISFGLVSIPVRLYTAASSQSVSFNLLHARCGNRI
                     RQQRFCPVCNEVVERDDLVKGYEFAKDQYVKVEDEELKALEDEASNAIEIAEFVPLSK
                     VDPVYFEKSYYLGPDKGGEKPYRLLSDAMAQAGKVALAKFVMRGKENLVLVRSAQNGL
                     MLHAMYFADEVRNFDEIGKGESAKVTDAETNLALRLIEELSSEQFSPEKYEDEYRDRV
                     LELINSKAQGKEITIAAPQAPRAQVIDLVAALKESLGKKVPQEKKPAVRAKPAQPEKA
                     KKRAQSGKK"
     gene            1926..2615
                     /locus_tag="VF089_10575"
     CDS             1926..2615
                     /locus_tag="VF089_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174451.1"
                     /translation="MRRPLAIIFNLVICFSISALPPLAWSLPLPSESPLQELIPLASW
                     QRQFKITEGKDRGKVVPLTAEPDLANGKRWKLVFGNYAAVHVVRQADGALTMKRLDLP
                     NSRSFIIYDSALPVMPSDVKSSDFIHRETGYKMYSLATGKLKRAGRVIHQVKPASMSQ
                     FDTPAGRLDGYYVEIDHRMDMEYYSQLHLTLGLGCQLDQGPVYGSGHYTLTKLGVFTE
                     SKTAGAALVAH"
     gene            2971..3516
                     /locus_tag="VF089_10580"
     CDS             2971..3516
                     /locus_tag="VF089_10580"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174452.1"
                     /translation="MTAMTEVHKGRQALMAGKAEVALAHFQRATGLDPKFLYFSTLPQ
                     SAITYTGRALYQLGRFSEARQAFEQATLQFADDSMARLYLGITFVRQGHQEPGFQETT
                     AGLKAIYQWLDYIEASLPQGIYWDSNREIRSEIERVLKQMTDRPLSAEQLIESAEWVG
                     NRMEWEIDAARRDEQASRNRD"
BASE COUNT          834 a         1023 c          984 g          885 t
ORIGIN      
        1 acaaaatctt cacagccgtg aagttgtggg agatgtcttc tgccctcgta ctaccccgac
       61 cgctgggagc gatcttattt tttcagataa agctttcgcg cagcgcggaa atgagccagt
      121 gcctcgacct tctcatttag agattcgcac aacaaagcta gattatagtg ggcatcggcg
      181 aagtccggat cgagctcgac tgcttgttcg taagcgcgac gggctgcttg gatttgccgt
      241 gcgtcttcaa gcaaaactcc taggttgaat cttggcgccg ggtccatcgg ggcgctatcg
      301 gcggcggcgc ggtagtgggc ttcggctttc ttgagctcac cctggtcatg atagagcttt
      361 cccaaattta ggtgcgcgtc cgccattttc gagtctcgct ctagcgccat gtggtaagct
      421 cgttgagctt ccttgattga actctcctcc aactccagcg ccagatcgaa ccaatgctga
      481 gcgcttagtt cactggcttg cggctttgcc ggagttatcg gcgcgttcag cttttgcacc
      541 acggcccggg catcgaagtt aaacagaaac tgtcctgaat cgggctgcca gcgtgccttg
      601 ccgtcccacg caatcactcg tcggttgtcg gcatagattt tcaggctcga gagatgccga
      661 tctcctggaa gctggcgctt caaggacaac agcatgcgga tgatgttttt gggtgggaca
      721 cgggagtcga ggagaccttt ggcggttttg agaaagagaa catcttgaaa tgtaaattgc
      781 agcgatttct tcttgccgcg gccaggcttg agaaatcccg cgcggacaaa cgagcggatg
      841 cgactacctg gaaggttgag gatcttcgcc acctcgccgg tggtgaacag tttcatttct
      901 taccggactg agctctcttt ttggccttct cgggttgggc gggtttcgcc cgaactgccg
      961 gcttcttttc ctgcggaacc tttttgccga gactttcctt gagcgcggcg accaggtcga
     1021 tgacttgggc gcgcggcgcc tggggcgcgg cgatggtgat ttctttgcct tgcgccttgc
     1081 tattgatcaa ctccagaaca cggtctcggt attcgtcttc atatttttcc gggctgaact
     1141 gctcgctcga aagctcctcg ataagcctga gcgccaggtt ggtttccgca tcggtgacct
     1201 tggccgactc gcctttgcct atctcatcga aatttcgcac ctcgtcggcg aaatacatgg
     1261 cgtgaagcat cagaccattc tgggccgatc ggaccagaac taaattttcc ttgccgcgca
     1321 tgacgaactt agccagggca accttgccgg cttgtgccat cgcatcggac aacaaacgat
     1381 aaggtttttc gccccccttg tcggggccga gataataact tttttcgaaa taaacaggat
     1441 cgacctttga aagcggtacg aattcggcaa tttcgatggc attggaggcc tcatcctcga
     1501 gcgcttttaa ctcctcgtcc tccaccttga cgtactgatc cttagcaaac tcgtatcctt
     1561 taaccagatc atcgcgctcg acaacctcat tacagaccgg gcaaaagcgc tgttgccgga
     1621 ttcgattgcc gcatctggcg tgaagcagat tgaagctcac cgactgcgag gaggcggccg
     1681 tgtagagtct aacgggaatt gatactaagc cgaaggaaat cgtaccggat ccaagtgaat
     1741 gcgggggcat gatgcttcct ccttcgagtc taaccagtca cggccacaac aaagacgcag
     1801 acagggcctg tctcttaata taacaggccg gggaaatttg aaatagtgcc tgggttgtga
     1861 tctagtggct ggaatctatt gtcggctaat gcgctgaagc gcagaacgag gagagaaaca
     1921 acccgatgag acgtcctctc gctatcatct tcaacctagt gatctgcttc tcaatctcag
     1981 cactgcctcc ccttgcatgg tctttgccgc tcccttccga atctccactc caggaactta
     2041 ttccgctggc cagctggcag agacagttta agatcaccga aggcaaggac cgcggcaaag
     2101 tggtcccgct cacggccgaa cccgatcttg cgaacggaaa acgatggaaa ctcgttttcg
     2161 gcaactacgc tgcggtccat gtggtgcgac aagcggacgg cgcattgacg atgaaacgcc
     2221 tcgacttgcc aaacagccgg agctttatca tctatgattc ggccctgccg gtaatgccca
     2281 gcgacgttaa atcctccgac tttattcatc gagagactgg ttacaaaatg tacagcctcg
     2341 ccaccggcaa gctcaagcgg gccggccgcg tgatccatca ggtaaagccc gcctcaatgt
     2401 cgcagttcga tactcccgcc ggtcgcctcg acggctacta tgtggaaatc gatcaccgca
     2461 tggatatgga atattacagc cagctgcatt tgacactcgg gctagggtgc cagttggacc
     2521 agggaccggt ttacggttcg gggcattaca cattgactaa actgggggtc tttactgaaa
     2581 gtaaaacagc tggagccgcg ctggttgcgc actgatcgac ccagtcgttt tctgatcaaa
     2641 catcggggat caccctacac gacaatcgcg attttaccgg actcaatcat aacgcggatt
     2701 gtggcttaac ggtcgtgagt cacttgcgcc ggacactgtt gagcacgata ccgtgcaccc
     2761 gtgttagtca ctcgtagccg cggtcatcgc ggcctctcgg ctgctctgtc tctggtcggg
     2821 acccaaaccg ataatcgagc gccattgacc ctcttcttgc accattaggc agcacatact
     2881 attcagatag tgccaaacct ggatatgagg gctgcctttc tcctagtgat ggttcttttc
     2941 gtcctcagcc atgctgcttg caaaaaaccg atgaccgcaa tgactgaagt gcacaaaggc
     3001 aggcaagcgc tcatggctgg aaaagccgag gtcgccctag cgcatttcca gcgcgccacc
     3061 ggactcgacc ccaagttctt gtatttcagc acccttccgc aatctgcgat cacctatacc
     3121 ggtcgcgctc tttaccagct gggaaggttt tcggaagcgc gccaggcctt cgagcaggca
     3181 acgttacaat tcgccgacga ttcgatggcg cgactttatc ttggtattac attcgtgcgt
     3241 cagggccatc aagaacccgg ttttcaggag actacggccg gattaaaagc gatttaccaa
     3301 tggctcgact atattgaagc cagtttgccc cagggaatat actgggacag caatcgagag
     3361 attcgatctg agatcgagcg cgttctgaag cagatgaccg atcgtccatt gagcgcggag
     3421 caactgatcg aaagcgcgga atgggtgggc aatcggatgg aatgggagat cgacgcagcg
     3481 cgacgggacg agcaagctag ccggaatagg gactaaggcg gttaacttca taaatcgctt
     3541 cacccgctca tgcgaagtaa gctctatttc cgttcacaaa gtgatacgcc ttattttgat
     3601 ccaaagaaat tgcgattctg actcgactga tgagcacaca atgaatcaat ccctaaaaga
     3661 aacccacaca ggaaaacttc gagccggcgt acggctgtct tgcggcttcc catcagtttc
     3721 tttgcc
//
LOCUS       DASUTG010000464         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2147811, whole genome shotgun sequence.
ACCESSION   DASUTG010000464 DASUTG010000000
VERSION     DASUTG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147811"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF089_10585"
     CDS             <1..196
                     /locus_tag="VF089_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174453.1"
                     /translation="IDEQQMIPDTKKRIALLQQAGKILMEDAPFVPLYNLASIYGAAK
                     NLAWKMRPDEKVLGWDMKIV"
     gene            614..793
                     /locus_tag="VF089_10590"
     CDS             614..793
                     /locus_tag="VF089_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174454.1"
                     /translation="MPIFSFLLKRRLLQSLFGGAGRSAQAGRAGGFLGGSFRKAAFAG
                     ITAMLVKRMLRRKSF"
     gene            919..1638
                     /locus_tag="VF089_10595"
     CDS             919..1638
                     /locus_tag="VF089_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEX6174455.1"
                     /translation="MAVEEEKTAAARTARQTKSLLLALMVLTFNTGLIDAVSYIGLGR
                     VFVANMTGNVVLLAFAAAGVSGLSVTRALSSLGGFLIGAAIGGKLGTSLAAGSRRRLI
                     LSAGILEALLIFAASVSAIGFDIRSSAPAHQLYTMIVLTALSMGLRNATVRQLRVADV
                     TTTVLTLTLTGLAADSPLAGGSNPRPARRIFSIVALFVGALSGAYLLRYGVALPLFVT
                     GACVLFTTAAYWVIAATVVKE"
     gene            1898..2356
                     /locus_tag="VF089_10600"
     CDS             1898..2356
                     /locus_tag="VF089_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174456.1"
                     /translation="MEITWNTSQHSKQTNVTPGETDYSAYSLQELFKLRYWFDAGREV
                     RGESQLEAEIRKRCAHIQERTSQKHPAINGSHHYRPYGLIFGVVFVVLSSGPFVAVKF
                     LEAVGIITDVAGDYALLTGLWAVLTLPFASMIYLIGGMMDAERVVKWFNL"
     gene            2742..3248
                     /locus_tag="VF089_10605"
     CDS             2742..3248
                     /locus_tag="VF089_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6174457.1"
                     /translation="MAQPKLPLPFEEIMQRHKREIMRYLLRLSSNPEDAADLFQETWL
                     RAYRAYPRLEPEDSVRPWLYTIASNLWRNRARDSARRIRVLVPDEKQLPAADAKDHRL
                     DCENEGYAAVHLRELIAALPAKQQQALHLRYFAGLDYTEIANAIDCSEESARANVSQA
                     MRKLKKRW"
     gene            3253..4056
                     /locus_tag="VF089_10610"
     CDS             3253..4056
                     /locus_tag="VF089_10610"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6174458.1"
                     /translation="MKLKTLMSSAAVDNPEEIFEELLRRSSAKLDKALQRIRRPQAAV
                     GIVKSKLGDLLVALSASGIVLNHYVGEISDIAAAVAKLRLQFDPVEDQRAVVTVAEEV
                     DRYLTGGGNTLRHHVDLTLVGNPFQKKVLDKLQAVPRGAVISYQTLGAAVGSASSARA
                     VGNALHNNPVPIYVPCHRVISSDGRIGGYGGGTGRKLQLLRSEGFALGDGAVRVPDNV
                     VWGHRRTQIYCRTTCRAAARVGRGRMFFFADRKAARQAGLRPCKICRPD"
BASE COUNT          886 a         1123 c         1120 g          963 t
ORIGIN      
        1 tatcgatgaa cagcaaatga tcccggacac aaagaagcgg atcgcgttgc tgcagcaggc
       61 gggaaagatt ctgatggaag acgcgccgtt cgtgccgctc tacaatctcg catccatcta
      121 tggcgcggcg aagaatctgg cgtggaagat gagacccgat gagaaggtcc tcggctggga
      181 tatgaagatt gtatagccgg gctggtgtcg cgatagcgca ggggaactgt tcgctcgttt
      241 gccctgttct caccccgcgt cggtttggct ataagcttct gcaccgcagc ttgagccatt
      301 cggtccacag atatcaacat ccaagtattg gtatgtatta acatcggcga ttagactttt
      361 tcatcgaagt tgacagctaa agatcgccgg ctcacccgga aaggcagcga actttaagcg
      421 gccatcctaa gttcaactcc ctagttctaa caggctatac aacgctggtg tcccgccgac
      481 aggggccaag gattagcaat gtcccttttg caatacactg atccatctca tcccgctatg
      541 cagacgggga gttctatggc atctcccttg cggatcaatg taggtcacaa tctctaaacc
      601 gaaggagcga acaatgccaa tcttttcttt tttgctgaag cgccgacttc ttcagagtct
      661 attcggtgga gcgggccgca gcgctcaagc cggacgcgcc ggcggcttct tgggcggctc
      721 gtttcgtaaa gccgccttcg ccggcatcac cgctatgctt gtcaaacgaa tgctgcggcg
      781 taaatcgttt tagccttctg atgtgcagcc tcgttgcgcc aggaccgatc aaatggacaa
      841 gcccgtcgtt catcgaaacc caactctagc gcacgccgga acaaagcgac cgtcggtgat
      901 tctttggtgt gaagagctat ggccgtagag gaggagaaga cggcggccgc tcggacagca
      961 cgtcagacta aatcgttact cttggcgctg atggtgctaa ccttcaacac cggactgatc
     1021 gacgccgtca gctatattgg ccttggacgt gtattcgtcg ccaacatgac aggcaacgtg
     1081 gttctcttgg cattcgccgc agccggagtt tccggcctat cggtcacgcg cgctttatcg
     1141 tctcttggcg gctttcttat cggcgccgca atcggaggaa agcttgggac aagcctggca
     1201 gctggcagta ggcggcggtt aattctgagc gccggaatct tagaagcgct cttgatcttt
     1261 gccgcctcgg tgagcgcgat tggattcgac atccgatcat cggcgccggc gcatcaactg
     1321 tacacaatga tcgttttgac ggcgttgtcg atgggattgc ggaatgctac ggtccggcaa
     1381 ctccgtgttg cggatgtcac gactacggtt cttacgctca cgttgaccgg cttggcggcg
     1441 gactctccgc tcgcaggagg cagtaatccg cggcccgcac gacgcatctt ctccatcgta
     1501 gccctcttcg tgggcgcact ctctggtgct tacctgttgc gctacggagt cgccttgccg
     1561 ctcttcgtca ccggcgcctg cgtgctcttc accactgcgg cgtactgggt gatcgccgcg
     1621 acagtggtga aagagtagcg ggctgctaaa aaccggcagt ccagcgccac tcgcgcaagt
     1681 gcctcatgga acctgctcgt gcaagccagt tcaatgaatc gtcaagaatc gaccacgatc
     1741 tccccgacgc gtcccttttt gctcgctcga tcagcatcgg cgcctttcta ctcggtgcgt
     1801 tgggcaaatt ttctttatag actggcggtt tataaccgta ctagcgagat cgacttaagc
     1861 aactctttgg tatgttcctt gctgatccgc accggccatg gaaatcacat ggaacaccag
     1921 ccaacactcc aagcaaacca acgtcacccc cggtgagaca gattactctg cctactcgct
     1981 tcaagaattg tttaaattac gttactggtt tgatgccggt cgcgaggtcc gcggagaaag
     2041 tcagctcgaa gccgaaattc ggaagcgctg cgcgcatatt caagaacgaa ccagccagaa
     2101 acatcctgcc atcaatggct ctcatcatta cagaccttac ggtctcatct tcggcgtagt
     2161 tttcgttgtg ctttctagcg gcccgttcgt tgcggtcaaa ttccttgagg cggtgggcat
     2221 cataactgac gttgccggag attatgcgct tttaaccggc ctttgggcgg tgcttacgct
     2281 tccatttgcg tcaatgatct atctgatcgg cggcatgatg gatgcagagc gggtcgtaaa
     2341 atggttcaat ttgtagttca tgcccgctgc cgttgaacta cgcttaagta ttcaatacat
     2401 acgcggcgaa atactgctgc gcgaatccga ataaacagat tgatcgcttc tcggttcatc
     2461 taagttttcc gttccccaca tcccgcaact tcggctccgg gctgcaatct aagtctcttc
     2521 tgcatatttt ttctcctgtt tcagccggcg atgctcctag cttaagactg tctagaaaag
     2581 agtgaccgca tagcaaccat cccgctcgcc cgctcgtgaa ttcgctgaac aagttcagtc
     2641 ccgtctactc ccgacttcga tgagagaacg acggagacct ggcagcgctt cataacgccg
     2701 ggacttttca cattcattct tctgtggtgt tttagatata tatggcgcaa cccaagctac
     2761 cgcttccgtt tgaagagatc atgcagcgcc acaaacggga aatcatgcgc tatctgctgc
     2821 gcctgtcgag taatcctgaa gatgcggcag acctgttcca ggaaacctgg ttacgcgcct
     2881 atcgcgccta tcccaggctt gaacccgagg atagcgtgcg tccgtggtta tacacgattg
     2941 cgagcaacct ttggcgcaac cgcgcgcgtg attccgcgcg ccgcatccgg gtgctcgtac
     3001 cggatgaaaa gcaactaccc gcagcggacg caaaggatca tcgattagat tgtgaaaatg
     3061 aaggctacgc cgcggttcat ctgcgcgagc ttatcgccgc tctgccggcc aagcagcaac
     3121 aggcgctgca tttgcgctac tttgccggcc ttgattatac ggaaatcgcc aatgcgatcg
     3181 actgttcgga agaaagcgcg cgggcaaatg tctcacaggc tatgagaaag ctcaagaaaa
     3241 gatggtgaag ctatgaaatt gaaaactctc atgagttcag ccgccgtcga caatccggaa
     3301 gaaatcttcg aggaattatt gcggcgctct tctgctaagc tcgacaaggc gctgcagcgg
     3361 attcgcagac cgcaagcggc tgtcggtatt gtaaaatcaa aactgggcga tctgctggta
     3421 gccctgagcg ccagcggtat tgtgcttaat cattatgtcg gtgaaattag cgatatcgcg
     3481 gcagccgtcg caaaattgcg gctgcagttc gatccggttg aggatcaacg ggctgtcgtt
     3541 acagtcgccg aggaggttga tcgttatctc actggcggag gaaatacgct acgtcatcat
     3601 gttgatctaa cgcttgtagg caatccgttc cagaagaagg tcctggacaa gctccaagcg
     3661 gtcccgcgcg gagcggtgat cagttatcaa acactggggg ccgctgtcgg ctccgcaagt
     3721 agcgcgcggg ctgtcggcaa tgcgctgcac aacaatccgg tgcctattta cgttccatgt
     3781 caccgggtaa tatcatccga cggacgaatc ggcggatacg gtggcggcac cggacgtaag
     3841 ctgcaattac ttcgcagcga aggttttgcc cttggggatg gcgctgtgag agttcccgac
     3901 aatgtggttt ggggacatcg gagaactcag atttactgtc ggaccacttg ccgcgccgcg
     3961 gcgcgggtgg gtcgtggacg gatgtttttc ttcgccgatc gtaaagctgc ccggcaggcg
     4021 ggcttgcgtc cgtgtaaaat ctgccggccg gactagcagt ccggtagata atcttgcagc
     4081 cgagaaagcg gc
//
LOCUS       DASUTG010000465         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1406504, whole genome shotgun sequence.
ACCESSION   DASUTG010000465 DASUTG010000000
VERSION     DASUTG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406504"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VF089_10615"
     CDS             <1..1189
                     /locus_tag="VF089_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX6174459.1"
                     /translation="TEWGEAINFDGPNSAPVREFFLANARYWISEFHFDGLRLDATQA
                     LFDASEKHILLELVETVRQAAAPKSTIVIGENEPQDSKLMRPAEDQGYGLDGLWNDDF
                     HHTAMVALTGHNEAYYADYLGSPQEFISSAKRGYLYQGQYYRWQKKRRGTPARGIKPM
                     AFINYLQNHDQIANSGRGWRVQRITDPALYRAITALFLLSPQTPMLFQGQEFAASAPF
                     TYFADHEGELGEKVKQGRREFLQQFPSLATEAMQSQLPDPTDPQVFASCKLDFSERSR
                     HHEIYALHQDLLHLRRADAAFNSQQSGAVDGAVLGDCAFVLRFFAADGKDRLLLINLG
                     ADTMLGPVPEPLIASAPGTTWQVLWSSEDPRYGGTGALPVESESGWLLIGKSAVVLAS
                     FPE"
     gene            1186..>2245
                     /locus_tag="VF089_10620"
     CDS             1186..>2245
                     /locus_tag="VF089_10620"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEX6174460.1"
                     /translation="MNPLLVRTLTDIPPIKLDLPELTHHPEWLVTNGLGGYASGTVSG
                     AMTRRYHGLLIAAFPAPLGRMVLLSDLNEEIYFPDGRFVQLSTRYGDSAEPTARLIEF
                     RLEMGLPVWRYQIDDWMIEKWLHLPYRQNTVYVTYKPFACPNDIRLKLRPLIQYRGHS
                     DDVDAQLEDPYVLTVLEDQYQISTGNLPPLRLLLYGKGEFVIDRKKIEKVRYLREEQR
                     GYHMIGELWTPGTFVLEAAAGEESTLLASAESWDVMRAMRPAEALAAERDRRTRLIAL
                     ADSRAHSGFAAELVLAADQFITVPTGRLPDQTRTHAAGDEMRTVIAGYHWFTDWGRDT
                     MISLEGLTLTTRRFLEAGW"
BASE COUNT          466 a          626 c          646 g          507 t
ORIGIN      
        1 aaccgaatgg ggtgaagcga tcaacttcga cggtcccaac tcggctccgg tgcgggagtt
       61 ctttttggct aatgcccgtt actggatttc tgagtttcac ttcgacggtc tgcgcttaga
      121 tgcgacccag gcgctgttcg atgcttctga aaagcacatc ctgctggagc tggtcgaaac
      181 cgtgcgccag gcagcggcgc caaaatcaac aatcgtgatt ggcgaaaacg aacctcagga
      241 tagcaagctc atgcgtccgg ccgaggacca gggttacggc ctcgacggat tgtggaacga
      301 tgattttcat cataccgcga tggtggcttt gaccgggcac aatgaagcgt attacgcgga
      361 ttaccttggt agtccgcagg agttcatttc cagcgccaag cgcggctacc tttatcaggg
      421 acaatactat cgatggcaaa agaaacgtcg tggcacacca gccaggggga tcaagcccat
      481 ggcgttcatc aattatcttc agaatcacga tcaaattgct aacagcggtc ggggctggcg
      541 ggtccagcgc atcaccgacc ccgcgcttta ccgcgccatc acggctctgt ttcttctctc
      601 accgcaaacg ccgatgttgt ttcaggggca ggagttcgcc gcttcggctc catttactta
      661 tttcgccgat cacgaaggcg agctcggtga aaaggtaaaa cagggtaggc gggagttctt
      721 gcaacagttt cccagcctcg ctacggaggc gatgcaatcg caacttcccg atccgactga
      781 cccgcaggtt tttgcctcgt gcaagctcga tttttcagaa cgaagtcgcc atcacgagat
      841 ctatgcgctg catcaggatc ttcttcattt gcggcgcgca gatgctgcct ttaactcgca
      901 acagtctgga gcagtggatg gggccgtact cggcgattgc gcgttcgtct tgagattctt
      961 tgcggcggat ggcaaagatc ggctgctgct gattaactta ggtgccgata cgatgctcgg
     1021 gccggttccg gagccgctga tcgcctcggc gcccggcact acctggcagg ttctttggtc
     1081 tagtgaggac ccgcgctacg gcggcaccgg cgcgctgcca gtagaaagtg aaagcggctg
     1141 gctgctgatt ggtaagagcg ccgttgtcct cgcgagtttc cctgaatgaa tcctcttctt
     1201 gtgcgcacgc tgaccgatat cccgccgata aagctcgatc taccggagct tacgcatcat
     1261 cctgaatggc tagtgaccaa tgggcttggc ggttatgcgt cgggaacggt atccggagcg
     1321 atgacccgtc gctatcacgg cctgttgatc gccgcgtttc cggcgccatt gggaagaatg
     1381 gtcctgctaa gcgatctcaa tgaagagatt tactttcccg atggccgctt tgtccagtta
     1441 agtacccgtt atggtgattc ggctgagccg actgcaagat tgattgagtt ccgcctcgag
     1501 atgggcttgc cggtttggcg ttatcaaatc gatgattgga tgattgagaa gtggcttcat
     1561 ctcccctacc ggcagaacac ggtttatgtc acctataaac cgtttgcttg cccgaacgat
     1621 atccgactca agttgcggcc gctgatccag tatcgcggcc atagtgacga cgtcgatgcg
     1681 cagctcgagg atccgtacgt tctgaccgtg ttggaggatc aatatcaaat ctcgaccgga
     1741 aatttgccgc cattgcggct gctgctctat ggcaaaggcg aatttgtcat cgatcgaaaa
     1801 aaaattgaaa aggtacgtta cctgcgcgaa gaacagcggg gctatcatat gattggcgag
     1861 ctctggaccc ccggcacctt tgtcctcgag gcagccgccg gcgaggagag cacgttactc
     1921 gcatcagcgg agtcctggga cgttatgcgc gccatgcgcc cggccgaagc gctcgcagcg
     1981 gaacgagatc gccgcacccg cctgattgca ttagcggaca gcagagcgca ctcaggcttc
     2041 gccgccgagt tagtcttggc ggcggatcaa ttcattaccg tgcccacggg tcgcctacca
     2101 gatcaaacgc ggacccacgc tgcgggcgac gaaatgcgca ccgttatcgc cggctatcat
     2161 tggtttaccg actggggccg cgacaccatg atcagtctgg aggggctgac cctgaccacc
     2221 cggcgttttc tcgaagccgg ctgga
//
LOCUS       DASUTG010000466         3656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_812656, whole genome shotgun sequence.
ACCESSION   DASUTG010000466 DASUTG010000000
VERSION     DASUTG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812656"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF089_10625"
     CDS             <1..634
                     /locus_tag="VF089_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEX6174461.1"
                     /translation="VCASGKNQSPIDLKGFIEADMKPLKLDYKAGIADIAHHGHGIAD
                     MVNNGHTVQVNHAPGSTLSVDGITFELKQFHFHSPSENKINGKQFPLEGHLVHADKDG
                     NLAVLAVMFQEGAANTLLAKLWEKMPNKAGEKSAPATRLSVSQLLPNERDYYRFNGSL
                     TTPPCSEGVRWFVIKKPGTASRAQIQQFSKAIGFANNRPIQSTNARSVLQ"
     gene            725..1738
                     /locus_tag="VF089_10630"
     CDS             725..1738
                     /locus_tag="VF089_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006917816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX6174462.1"
                     /translation="MIKTASKIFVRILLGIIIAGMTAWATGAIYYSPLPGESLRAVLA
                     ASFALATALAFLLLHNRRRTLVGFLIAFALLVFLFFQIPASNDRDWQPEVAVTPYADI
                     NGDLVTIHGVRNFDYRSETDFTPRWETRTYDLRKLDSANIIAVYWAGKAIAHIMVSFG
                     FEGKDYLAVSIETRKEKGESYSTLAGFFRQYELYYVVADERDVIGVRTTYRQPQEDVY
                     VYGVNGKLENIRRFFLDYVKSINQLHRNPTYYNTLTTNCTTSILMHARINPESPPMSW
                     KILLSGYVPDYLYELGRLDTTKTFLELEKLSHVNARARAADKDPAFSQRIREGLPKPA
                     LLR"
     gene            1735..3639
                     /locus_tag="VF089_10635"
     CDS             1735..3639
                     /locus_tag="VF089_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6174463.1"
                     /translation="MKSSRLLFRDRVLNGSRSRRPKLVYVSALVGLLLLTSGCATPIG
                     VVRGTTQEMHYALTANVLSAGEPSTWSKQVLHGTNLSERFNTDPAATLGILHKTLGRL
                     SEDRLPDRLFALAELSFLYAENSAERSHYLAAAVYAYALLVPDNGQRLDPLDPRVRLA
                     ADIYNRGLTRGLSTPIEAEVVLEAGSQPLPFGELVLTTDPADFFWSGYRFKRFIPVGE
                     FVVRGFSNRYRQAGVGAPLAAELEAVDSGPAAEAARKRIPPRTRVPVTAFLRIATPRR
                     GIVEGTVQGKLELYAADQASTVAVGGRDVPLELEPTAALAYQLEGAPIWDFEIAGFRF
                     ADKFPGVGDGLMMMRPYRRGRIPIVLVHGTASSPVRWAELFNEISHDPVVSGRYQFWL
                     FQYNTGQPILYSAMQLRHALRNVFSEIDPLGEDEALRQMVMIGHSQGGILTKLMAVNS
                     GTRFWNNLSTEPFADLEMAPETRELLQEAMFFEPVPTLRRVVFIATPHRGSYQATGWA
                     LDLIRRFITLPGTLVTQFQGLLKSQAFASLGVREMPTSVDNMSPGHPFLRALNELPID
                     PAITAHSIIAVLGDGPITGKTDGVVAYESAHIEGVASEVVVRSGHSTQGHPETVEEVR
                     RILREHVGSK"
BASE COUNT          779 a         1047 c         1078 g          752 t
ORIGIN      
        1 agtatgcgcc agcggcaaga accagtcgcc aatcgatctc aagggattca tcgaagcgga
       61 catgaagccg ctcaagctcg attacaaagc gggaatcgcg gacattgccc accatggaca
      121 tggcattgcg gacatggtca acaacgggca cacggtgcag gtcaaccacg ctcctggaag
      181 cacgctatcg gtggacggca tcacgttcga attgaagcag ttccacttcc attccccgag
      241 tgaaaacaag atcaacggca agcagtttcc actcgaagga catctggtgc acgccgacaa
      301 ggacggcaat ctcgccgtgt tagcggtgat gtttcaggaa ggcgccgcaa acacactgct
      361 cgccaaactt tgggaaaaga tgcccaacaa ggccggcgag aagagcgcgc ctgcgactag
      421 gctgagtgtt tcgcaactgc tacctaacga acgcgattat tatcgcttca acggatcgct
      481 cactacgccg ccgtgcagcg aaggcgtacg ttggtttgtc ataaagaagc caggtacggc
      541 ctccagggcg cagatccaac agttttccaa ggcgatcggg ttcgccaaca atcggccgat
      601 tcagtcgaca aacgcgcgct ccgtcctgca ataacggtaa agttcggtcg aagggaaaag
      661 gctcagaaga ggagcgcgcg atgcctcaaa ccgatgggat tgggcagaga agaaagaaaa
      721 aactgtgatc aagacggcgt ctaaaatttt cgttcgcatc ttgcttggga tcatcatagc
      781 cgggatgacc gcttgggcca cgggtgcaat ctattattcg ccactgccgg gtgaatctct
      841 tcgggccgtt cttgcagcat cattcgcact cgcaacggcg ctggcgtttc tgctgttaca
      901 taatcgccgc cggactttgg taggtttcct catcgcattc gcgctgctcg tgtttttgtt
      961 ttttcaaatt ccggcttcca atgatcgcga ctggcagccg gaagtggctg taacgcctta
     1021 tgctgacatc aacggtgatc tcgttacgat tcacggcgtg agaaatttcg actaccgcag
     1081 tgagaccgat tttacgccgc gctgggaaac gcgaacgtac gatcttcgca aactcgattc
     1141 ggcgaatatc atcgcggttt actgggcagg taaggcaatt gcgcacatca tggtcagctt
     1201 cggatttgaa ggtaaggact acctcgcggt gtccatcgag acgcgcaaag agaaaggcga
     1261 gagttattcg accctcgccg gtttttttcg acagtacgag ctgtactatg tggttgccga
     1321 tgaacgcgac gtgattggcg tgcgcaccac ttatcgccag ccgcaagagg acgtgtatgt
     1381 ttacggagtc aacggcaagc ttgaaaatat ccgacggttc tttctcgact acgtaaagtc
     1441 gattaaccaa ctgcaccgaa atccgaccta ctacaatacc ttgacaacca actgcacgac
     1501 cagcattctc atgcacgcgc ggattaaccc ggagtcgccg cccatgtctt ggaagatcct
     1561 gctcagcggc tacgttcccg actatcttta cgagctgggg cggctcgaca cgacaaaaac
     1621 gtttctagag ttagaaaagc tgtcgcacgt caacgcgcgc gcccgcgcgg ctgacaaaga
     1681 cccggcgttt tcgcagcgca tcagggaggg gctgccgaag ccggcgcttc tgcgatgaaa
     1741 agttcacgac tgttattcag agatcgcgtt ctgaacggca gccgatcgcg ccggcctaaa
     1801 cttgtctacg tcagcgcgct agtcggtctc ctcctgctca ccagcggctg tgccacgccc
     1861 atcggcgttg tccgcggaac cacgcaggag atgcactacg cgcttacggc gaacgtatta
     1921 tcggcggggg aaccaagcac ctggtccaag caggtgctgc atgggaccaa cctaagcgaa
     1981 cggttcaaca ccgatccggc agccacgctc ggtatactgc acaagacgct gggccggtta
     2041 agtgaagatc gtttgccgga tcggctcttt gcgctcgccg agctttcatt tctgtacgcc
     2101 gagaacagcg ccgagcggtc gcactatctg gccgccgccg tgtatgcgta cgctttgctg
     2161 gtgcctgata acggccagcg gttggatccc ctcgatccac gcgtgcgcct ggccgccgac
     2221 atttataatc ggggactcac caggggttta tccacgccca ttgaagccga agttgtcttg
     2281 gaagcgggtt cgcagccgtt gcccttcggc gagctcgtgc tgaccactga cccggcagac
     2341 ttcttttgga gcggctatcg gttcaaacgt tttatccccg taggcgagtt tgtcgtgcgc
     2401 gggtttagca atcgttatcg acaagcggga gtcggagcgc cgctcgccgc cgagctcgaa
     2461 gcggtcgact caggcccggc cgccgaagcg gcgcgcaaac ggataccgcc gcggacgcgg
     2521 gtgcccgtga cggctttttt gcggatcgcg acgccgcgcc gcggtatcgt cgagggaact
     2581 gtgcaaggca agctagagct ctacgcggcc gatcaggcgt ctaccgtggc tgtcggcggc
     2641 cgcgatgtgc cgctcgagct ggagccgacg gcggcgctcg cctatcagtt ggaaggcgcg
     2701 ccgatctggg acttcgagat tgccgggttt cgcttcgccg acaagttccc gggcgtcggc
     2761 gacggcctga tgatgatgcg cccgtaccgt cgtggccgta ttccgatagt gctcgtgcac
     2821 ggcaccgcat cgagtccggt acgctgggcc gagctgttca acgagatctc gcacgacccg
     2881 gtggtcagcg gccgctacca gttctggctt tttcagtaca acaccggcca gccgattctg
     2941 tactccgcca tgcagctgcg ccatgcgttg cgcaacgtgt tcagcgagat cgatccgttg
     3001 ggagaagacg aagcattgcg gcagatggtc atgatcggcc acagccaggg cggcatactg
     3061 accaagctca tggcggtcaa cagcggcacg cgcttttgga ataatctgag cacagagccg
     3121 ttcgccgacc ttgagatggc gcccgagacc cgggaactgc tccaagaagc gatgtttttc
     3181 gagcccgtgc caacgttacg acgagtggta ttcatcgcca cgccgcaccg gggcagttac
     3241 caggcaacgg gctgggcact ggatctcatc cggcgattta tcaccctgcc ggggaccttg
     3301 gtgacgcagt ttcagggttt attgaaatcg caagcctttg ccagtctggg ggttcgcgaa
     3361 atgccgacgt cggtggacaa catgagtccg gggcatccgt ttcttagggc gctcaacgaa
     3421 ctgccgatcg accccgccat cacggcgcac tcgatcatcg cggtacttgg ggatggcccg
     3481 attaccggca aaaccgacgg ggtggtcgcc tacgagagtg cgcatattga aggcgtggct
     3541 tcagaggttg tggttcgttc gggccattcg actcaggggc atcccgaaac ggtcgaagaa
     3601 gtgcgccgaa ttcttcgtga gcatgttggc tcaaaataaa gagtgccgga gcattg
//
LOCUS       DASUTG010000467         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_665314, whole genome shotgun sequence.
ACCESSION   DASUTG010000467 DASUTG010000000
VERSION     DASUTG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665314"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1216
                     /locus_tag="VF089_10640"
     CDS             122..1216
                     /locus_tag="VF089_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174464.1"
                     /translation="MRTSNLRLLSWLIGCSFFIAGWTQGLAAEKAPYYQGKTLTIVIN
                     FAAGGPTDIEGRVFGKHLGKHIPGQPSVVVQNMGGGGGITGVNYIGEVAKPDGLTAGY
                     FTGSLFHHQTKDPGLRVDLGKFGFITGVHGVTISYVRADTPPGIKKPADFVKAERFRA
                     AGLSASSSKDVRFRLSFDLLGLKYDYVTGYSSSSTARLAVQRNEAQYHDETLPAYRAA
                     VEPQMVKTGEVTALYYTDLVSPDGDIIVSPDVPELLPFTHYYRQVFGKMPSGIKYEAL
                     KAANMSSTNMTRLIMLPPNSPAAAIATLRQAIVSLSKDSDFNAEAMKAMRFQPRFEVG
                     EQGERMFQRVSQTPPEIVSFIRQYIDQVNK"
     gene            1514..2560
                     /locus_tag="VF089_10645"
     CDS             1514..2560
                     /locus_tag="VF089_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174465.1"
                     /translation="MKHFGVLLLAFLLCPAGLQAQARKPATISELVTYSGADREQVLY
                     AGAKNEGKLTWYTSLAGDSYKAMVKAFETKYPGVKIEAYRVSGSDINTRMFEESKAKR
                     YIADTVETTEGNLMFLRDALLLRPYHSPHFASYPEEAKEKSERGLYFWAIARESYIGF
                     AYNKNLLSKSAVPKNFEGLLHPELKGRMGVSLSDPIYKVIGAMLNTKGQEFVKKLKVQ
                     EIALHTIIPPALLDLIASGEVLASPGIFRNHVLVAIEKGAPVEWLPLDLVPTNVGGAA
                     IAAQPPHPHAALLLADYLLGAEGQALLGKYHYGSATKELGFKRWRPDHGISTDKYEKD
                     LERWEKLLKEISRR"
BASE COUNT          676 a          731 c          756 g          602 t
ORIGIN      
        1 gccaagaaag atcaagaggt aaccgagccg ataagcctta gcagcagcta gagccgataa
       61 cattgtctaa acttttcaat cagaggtcct gtcgatcaac ggatcaacga aaggaatcat
      121 aatgagaacg agtaacctgc gattgttgtc atggctcatc ggctgctcgt ttttcattgc
      181 gggatggaca caaggattgg cggctgaaaa ggcgccctat tatcagggca agactttaac
      241 catcgtcatc aactttgccg ccggcggccc caccgatatt gaaggacgag tcttcggtaa
      301 gcatctcggc aaacatatcc cgggacagcc ttcggtcgta gttcagaaca tgggcggtgg
      361 cggaggcatc acaggagtca actatatcgg agaagtcgcc aagccggatg ggctcacggc
      421 cggctatttc accggcagtc tttttcacca tcagaccaag gacccaggtc ttcgggtgga
      481 cctcgggaag ttcgggttta tcaccggtgt gcacggcgtg acgatttcgt atgtccgagc
      541 agacacaccg ccagggatta aaaaaccggc agacttcgtg aaagcggagc ggtttagggc
      601 cgcagggtta agcgccagca gttccaaaga tgtgcgcttt cggttgtcgt tcgacttgct
      661 gggcctgaag tatgattacg tcaccggtta cagctccagc tcaaccgctc ggctggcggt
      721 tcaacgaaac gaggcgcaat accacgacga aactctccca gcttaccgcg ctgccgtcga
      781 accgcagatg gttaaaaccg gtgaagtgac cgcgctttat tatactgatc tggtctcccc
      841 ggatggagat atcatcgtca gcccggacgt gccggagttg ttgcccttca cacattatta
      901 caggcaggtg tttggcaaga tgccatccgg aataaagtat gaagcgctca aggcggccaa
      961 catgtcgagc accaacatga cgcgcctgat catgctgccg ccaaactcgc ccgccgcagc
     1021 catcgcaacg ctccggcagg ctattgtctc cttatctaaa gactccgact tcaatgccga
     1081 ggcgatgaag gcgatgcgtt ttcaacctcg cttcgaggtc ggtgagcagg gcgagcgcat
     1141 gttccagcgg gtttcacaaa cgccgccgga gatcgtaagt ttcatccgcc agtacatcga
     1201 ccaggtaaac aagtaatctc caatttcctc gccagcaggc gcaatcggcc gatgcccgac
     1261 gaggattctt ccttcgtcgg gctgggttgg agagcgacac tttgcccctt catcgatctg
     1321 tcttgattca ccggtgaacg gcgcagattg gctcgggatt gcgcagttcc tttggagccg
     1381 ctgacacaag ctaatcgtcg aacgaaatga cttcttggaa agcatacggc acaattcgct
     1441 ggaaacgcgg caggcaccgc ttgtcagttc acgcaggcag aggtaagaac ggtacaggag
     1501 ggaagtaggt tcgatgaagc actttggtgt tttgctttta gctttcttgc tatgccctgc
     1561 gggcctgcag gcacaagccc gcaaaccggc aacgatctcg gagctggtga cttacagtgg
     1621 cgccgatcgt gaacaggttc tgtacgccgg cgctaaaaac gaaggcaagc taacctggta
     1681 tacctcactc gccggtgact cttataaagc tatggtcaaa gcattcgaga ccaagtaccc
     1741 gggcgtaaaa atcgaggcct atcgagtatc gggttcggac atcaatacca gaatgttcga
     1801 agaatcgaag gcgaaaagat atatagccga cacggtggaa accaccgaag gcaatcttat
     1861 gttcctgcgc gatgctcttc tcctgcgtcc ctatcattcg ccacattttg cttcctatcc
     1921 agaagaagcc aaagaaaaaa gcgagagggg cctctatttc tgggccatcg cgcgtgaatc
     1981 ctatatcggc tttgcctata acaagaacct gctctccaag agcgcagtgc cgaaaaattt
     2041 cgaagggctg ctccatcctg agctcaaggg gcgcatgggc gtatcgctca gcgatccgat
     2101 ctacaaagtg atcggcgcta tgttgaacac caaggggcag gagtttgtca aaaagctaaa
     2161 ggtccaggag atcgcgctgc atacgattat tccgccggcc ctgctcgatt tgatcgcctc
     2221 gggtgaagtc ttggcctcgc cgggaatttt tcgtaatcac gtattagttg cgatcgagaa
     2281 aggcgcgccg gtggagtggt tgccattgga tctcgtaccg acgaatgtgg gcggagccgc
     2341 aattgccgcc caaccgccgc atcctcacgc cgcgctgcta ctggcggact atcttttggg
     2401 agccgaaggg caagcgctat tgggaaaata ccattacggc agcgccacca aggaactcgg
     2461 ttttaagcgc tggcggccgg accatggaat cagcaccgac aaatacgaaa aggacctgga
     2521 acgttgggag aagctactca aagaaatcag ccgcaggtga attattgcgt tgcctcggcg
     2581 attcgacttc agctaggaag ttggtttaaa ccaattgttc ttctcaacca ttgacttagg
     2641 aacaaggaaa aggaaattgt aaaagcctaa gagggcctgc ctatgcttgc cgtaatgatg
     2701 cgcaatccag cccagtggct gtttctctta accagcgtga ctttggcgta tctggtgctg
     2761 ccgcc
//
LOCUS       DASUTG010000468         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2148123, whole genome shotgun sequence.
ACCESSION   DASUTG010000468 DASUTG010000000
VERSION     DASUTG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2148123"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF089_10650"
     CDS             <1..404
                     /locus_tag="VF089_10650"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6174466.1"
                     /translation="IGIIEEVNAMEPIRFIDLPDTEAANKILAPYGASIVESQPTTGA
                     KGGTHVIGYALHLVSSNKVSDNTIYTLTKTWWNNLAELEKLHPLFKRWSKEHQALTNF
                     TVPYHPGAIKFYKEAGVWTAKHEARLKEICR"
     gene            458..2431
                     /locus_tag="VF089_10655"
     CDS             458..2431
                     /locus_tag="VF089_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX6174467.1"
                     /translation="MVDIEESVRSRELRGPLAWAAKAALLAIPITGIIFLLSVPQMFG
                     WLFFNEQYMGLFLGLALFATFLLVPHKRGASQHQVPWFDLIFALAGLSVGLYILIEYP
                     QLVNTLGDIHQTGVILGSITILLLAEATRRVVGWPLVIIAAAFVFYALFAYLFPGDFY
                     GRGWSIGRLATYLYLDANGIFGLALQVGSSIVLIFILFGEVLNAVGGARFLSDFSQAL
                     MGRFRGGQAKIAIVSSSLFGNVSGSAVANVVVDGAFTIPMMKRAGYPAPQAAAVEAVA
                     STGGQIMPPVMGAAAFLIAEYLGIPYAEVALAALVPAVLYYVTLFIQVDLLAARNGIY
                     GLPRAELPRLAPVMRRSAGFVVPLAVLISAMFFMNRRAEEAGLWATLSALIIGFLTPS
                     IRIGLRGLLNVMISMGRSMLEIAAITGLAGVVIGVLQLTGLGFTLTLTLLDVGQSSAI
                     LLLVLTAIVSIILGMGMPTTAVYVLLAVLVAPGLSKLGILPIAAHLFIFYFGMLSMVT
                     PPVCLASYAAASIGKTDPIKTGWEGMRLCSIAYLIPFLFVFSPTLLLIGPWYAVVISV
                     ATAVVGAVLLGVGLVGYLFSPIGPIKRLLFVLAAAGLLIPVVETGTYANLTWTVNTGG
                     LLLAAVLIAIEWIARSRRRESKIAAAAKASVRG"
     gene            complement(2477..>2764)
                     /locus_tag="VF089_10660"
     CDS             complement(2477..>2764)
                     /locus_tag="VF089_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174468.1"
                     /translation="RRQSRSVIDPLNYLSGQVPVNARGLLRTREGRGQLYLRSADIGG
                     VPLPKPLLQELVGYFSRTPQNPNGFDIDAPFDLPSKIREISVGAGESVVIQ"
BASE COUNT          483 a          804 c          804 g          673 t
ORIGIN      
        1 gcatcggcat catcgaagag gtcaacgcca tggagccgat tcgcttcatc gatcttcccg
       61 acaccgaagc ggccaacaag atcctggctc cttacggagc ctctatcgtc gagtcccaac
      121 cgactaccgg tgcaaagggc ggaacccacg ttattggcta tgcgctccat ctggtcagtt
      181 ccaacaaggt aagcgacaac accatttata ctttgaccaa gacctggtgg aataatctgg
      241 ccgaactgga gaagctgcac ccgcttttca agcgttggtc caaggagcac caagcgctca
      301 cgaactttac cgtcccctat catccgggag cgatcaaatt ctacaaggag gcaggcgttt
      361 ggacagccaa gcacgaggcc cgtttgaaag aaatctgtag atagccgccc tgtggccgag
      421 ccgagcattt cggctcggct tcgcgaaagg ttcttcattg gtcgatatcg aagaaagcgt
      481 tcgctcccgg gaactgcgcg ggccgctagc ctgggccgct aaagccgctc tgttggctat
      541 tcccattacg ggaataattt ttctcttgag cgtgccgcaa atgttcggct ggcttttttt
      601 caacgagcaa tacatggggc ttttcctcgg cctcgcgctg tttgcgacct ttttgttagt
      661 tccccataaa cgcggcgcca gtcagcatca ggttccctgg ttcgatctga tctttgcatt
      721 ggccggtctc agcgtcgggc tctatatatt gatcgagtat ccgcagctgg ttaacaccct
      781 cggcgacatc caccaaaccg gcgtcattct cggctccatc acgattttgc ttttggccga
      841 agcgacccgc agagtggtgg gttggccgct cgtcatcatt gccgcggcct ttgtcttcta
      901 tgcgcttttt gcctatctgt ttcccggcga tttctacggc cgcggctggt cgatcggccg
      961 gcttgccacc tatctgtatc tcgatgccaa cgggattttt ggactcgcac tgcaagtagg
     1021 atcgagcatc gtcctgatct ttatcctttt cggcgaggtg ctgaacgcag tcggcggcgc
     1081 caggttcctc agcgactttt cccaggctct gatgggccgt ttccgcggcg gccaggccaa
     1141 gatcgccatc gtttcctcca gcctgttcgg caacgtcagc ggcagcgccg tggccaatgt
     1201 ggtggtggac ggcgctttca ccattcccat gatgaagcga gccggctatc cggcgcctca
     1261 agccgcggcg gtcgaagcgg tggcctccac cggcgggcag atcatgccgc cggtcatggg
     1321 cgccgccgcc tttctgatcg ccgaatatct cggcattccg tatgcggagg tggcgttggc
     1381 ggcgctggtg cccgcggtgc tctattacgt cactcttttt attcaggtgg atttgctggc
     1441 ggcgcgcaac ggcatctacg gcctgcctcg cgcggaattg ccgcgtttgg caccggtgat
     1501 gcggcgctct gccggctttg tcgtgccgct ggcggtgttg atcagcgcga tgttttttat
     1561 gaaccggcgc gccgaagagg cggggctgtg ggcgacgttg tccgccctga tcatcggatt
     1621 tcttacaccg agtatccgca tcggcctgcg cggcctgtta aatgtgatga tcagcatggg
     1681 gcggagcatg ctggaaatcg ccgccatcac cggccttgcc ggcgtcgtta tcggcgtgct
     1741 gcagttgacc gggctgggtt tcacgctgac actgactttg cttgatgtcg gtcagagcag
     1801 cgcgattctg ctgctggtgc taaccgcgat cgtcagcatc attctcggca tgggcatgcc
     1861 gaccaccgcg gtctatgtat tgctcgcggt gctggtcgcg ccgggactgt caaagctcgg
     1921 catcctgccg atcgccgccc atctgttcat tttctatttc ggcatgctga gcatggtcac
     1981 gccgccggta tgtctggcga gttatgccgc ggcatctatc ggcaaaaccg atccgatcaa
     2041 gaccggctgg gaagggatgc ggctgtgctc gatcgcctat ctaattccct ttctgttcgt
     2101 gttctcgccc acgttgctgt tgatcggccc gtggtacgca gtggtgatat ctgttgcaac
     2161 cgctgttgtt ggcgcagttc ttttgggcgt cggtttggtc ggttatttgt tcagccccat
     2221 cggccctatc aaacggctgc ttttcgttct tgccgcggcc ggtttgctta ttccggtagt
     2281 cgagaccgga acctatgcga atcttacctg gaccgtaaat acgggtggtt tgttactggc
     2341 cgccgttctg attgcaatcg aatggatagc acgatcgcga cggcgggagt cgaaaatagc
     2401 ggcggcagcg aaggcttcgg tccgtggctg ataggctcaa ggatacggta gtccggcttc
     2461 tacttcgccc ggagcgctac tgaattacga cactctcacc ggcgcccacc gaaatctcac
     2521 gaatttttga cggaagatcg aacggcgcgt cgatatcgaa gccgttaggg ttttgcggcg
     2581 tacgcgagaa gtaaccgacg agttcctgca gtaacggttt aggcaacggc actccaccga
     2641 tgtcagccga gcgcaaatac aattgccccc tgccttcccg ggtccgcaag aggccccgcg
     2701 catttaccgg gacttgacct gataagtagt tgagcggatc gatcacgctg cgcgattggc
     2761 ggcg
//
LOCUS       DASUTG010000469         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1702599, whole genome shotgun sequence.
ACCESSION   DASUTG010000469 DASUTG010000000
VERSION     DASUTG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702599"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF089_10665"
     CDS             <1..241
                     /locus_tag="VF089_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174469.1"
                     /translation="WPIHLPPGTPPEKVAILREALNKTWKDPEFTQTFKKLMGREPTP
                     LTGEEVEKAVQELPRDPAVVELYKKLADAGPLPPR"
     gene            608..1963
                     /locus_tag="VF089_10670"
     CDS             608..1963
                     /locus_tag="VF089_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174470.1"
                     /translation="MHRILFIEKEDRTRALLQCRLKAEAKIKFAPSMEAAWEKLSSRK
                     FDLILWNTVNDAAAETSLRETLNRLSTNVCSSRIVIFADSEALPAAGLPAGNVCFDNL
                     PTDDEDLVALIQSNLPIKPRPDRSNSSPDNFALPIEFEGLIAVSLPMGAVLRRAIEAA
                     NVDMPVLITGETGTGKDLIAAAIHKRSRRKDNPYVIVNTGAMSPQLIASEIFGHEKGA
                     FTGAEEAHQGIFEQAQGGTVFLDEIATMDEKTQVSLLRVLEEKTFQRVGGIKKYSADV
                     RIIAATNEDLEKNVTEKRFREDLFYRLNVLHISVPPLRDRPGAITVLTDHFLSHFAPM
                     FGKEVERVAPETYRLLRRYRWPGNVRELKNVIQAALLMLDGNELTPDCIPERIRDAAL
                     GDEGRGEPSCSFRVGASLHSVEKEFIRVTLTFAGGNKKLAASILGISRRALYNKLKNH
                     D"
     gene            2359..2652
                     /locus_tag="VF089_10675"
     CDS             2359..2652
                     /locus_tag="VF089_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174471.1"
                     /translation="MFAVLTDAIECFQKYYGANAHRGRRLFTEAEAWISRRDYAWPYS
                     FENICETLNIDPNYLRLGLTRWRIHHEAQKNTGLCVRKTLRYQNTVKQNRVCI"
     gene            2776..>2984
                     /locus_tag="VF089_10680"
     CDS             2776..>2984
                     /locus_tag="VF089_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174472.1"
                     /translation="MEMSSETHGSGIGNKVMLVGIDNNHVIEKRLQAAGCEVIKVTDE
                     EAALDHVRHEIFDKAVMVSTGSLIDT"
BASE COUNT          779 a          783 c          780 g          642 t
ORIGIN      
        1 ctggccgatt catttgccgc cgggaacccc acctgagaag gtcgccattc tgcgcgaagc
       61 tctgaacaag acttggaaag atccggagtt cacacaaact tttaaaaaat tgatgggcag
      121 agaacccact cctctgactg gagaagaagt ggaaaaggcg gtccaagagc tgcctcgtga
      181 cccagcggta gttgagctat acaagaaatt agccgatgcg ggtccactac caccgaggta
      241 aggagaagcg acggcagcaa ttcagcgtcc taacaaaatg acagcacccg atcgagtgca
      301 cgccccacga atattgcgct ttcaataagt agttcaaccg acaacagttc cggggaagtg
      361 gtggtcgcac tcgaatttac cacaagtgaa ccggagagca tcaggtagac catggcgcac
      421 acaggccgcg ccggacgcgc agtcgttaga cggttgagcg gctcatcaag cctaactgat
      481 acgtcgcggt tgccaggcac atgcccaaca tcattcgcca tcaattctag taggagctta
      541 tttgacttgc taggacttta gtattaaacc cctgacacag tcgatccttg cagcatagga
      601 ttcatcaatg catcgcattc tgtttatcga gaaggaagac cgcacccgag ctctactgca
      661 atgccgcctc aaggcagaag caaaaatcaa gttcgctcct tccatggaag ccgcctggga
      721 aaagctgagc agtcgcaagt tcgatttaat attatggaac acagttaatg atgcagcggc
      781 agagacaagt ctgagagaga cgctcaaccg gctctcaacc aacgtttgca gcagcaggat
      841 tgttattttc gctgattctg aggccttacc ggccgcagga ttacccgccg gcaatgtttg
      901 ctttgataat ctaccgacgg acgacgaaga tttagttgcg ttaattcaga gtaatcttcc
      961 gattaagcca cggccggatc gaagcaactc tagccctgat aacttcgcac tgccgattga
     1021 gtttgaggga cttatagcgg tgagcctgcc gatgggcgcc gtgctcaggc gcgcgattga
     1081 ggccgcgaat gtcgacatgc cggttctcat aactggcgag accggaaccg gcaaagatct
     1141 cattgccgcc gccattcaca agcgcagtcg gcgcaaggac aatccttacg tgattgtaaa
     1201 taccggagcg atgtcgccgc agctgattgc cagcgagatc ttcgggcatg aaaaaggcgc
     1261 atttaccggg gcggaggaag cgcatcaggg gattttcgaa caagctcagg gaggcactgt
     1321 ttttctcgat gaaatcgcca ccatggacga aaagactcag gtgagtttgc tccgcgtcct
     1381 ggaagaaaaa acatttcagc gcgtcggcgg tatcaagaaa tatagcgccg acgttcggat
     1441 catagctgca acgaacgaag atctggaaaa gaacgtcact gagaaacgat ttcgcgagga
     1501 tctcttctat cgactcaacg ttctgcacat aagcgttccg ccgttgcgcg accgaccggg
     1561 cgccatcaca gtgctgacgg atcattttct ttcccacttt gcgccgatgt tcggaaaaga
     1621 ggttgagcga gtcgcgccgg aaacctaccg attgctgcga cgctaccgct ggcccgggaa
     1681 cgtccgtgaa ctcaagaacg ttatacaagc ggcgctcttg atgctcgatg gcaatgaact
     1741 aacaccggat tgcatcccgg aacggatccg ggatgccgcc ttaggggacg aaggccgcgg
     1801 cgaaccgagt tgctcgtttc gagtcggtgc ctcgctgcat tcggtggaga aggaattcat
     1861 tcgggtaaca ctcacctttg ccggcggaaa caagaagcta gcggcaagca tcctcgggat
     1921 cagccgacgt gctctttaca acaagctcaa gaaccacgat tgacgtcgat gtgcggatac
     1981 tgcgcacgcg attaaccctc aaaaatctct aacacgttgt ttttaaaaac attgtgttcc
     2041 ttgggaattc gtcaaaggcg ctcgcggtgt aaagaatgaa cacactttct acctcctaag
     2101 tgtagaaact accgcttttc gcaaccagca gcttaccgcg cgcgaagttg tccccgtaag
     2161 ttaccgcata tccacggatt gcctcactgg ttctattctt gctctcggag ccttgaaccc
     2221 ggagggttaa tgaatagagc ggcggcaact atacggtcca acccgatcaa ctcgttcttc
     2281 gagcctgaca tccttacttc acatcagttt ttccaacact atcgccacaa ggcacatttc
     2341 gatccggaag agaaacttat gttcgcggtt ctgactgacg cgatcgagtg ctttcaaaaa
     2401 tattatggcg ccaacgccca ccgaggccgc aggctcttta cggaagctga ggcctggatc
     2461 tccaggcggg attacgcctg gccctattcc tttgagaata tctgcgaaac attgaacatc
     2521 gatccaaact acctgcgcct cggtttgacc cggtggcgga tccatcatga ggcgcagaaa
     2581 aacaccggct tatgcgttcg taagacgctg cgctaccaaa atacagtgaa acagaatcga
     2641 gtgtgcatct aacaaagatg agagatccag cgccccgtca gggactcacc gtacggtctg
     2701 cgcatcctca actccggctg gcaagctcaa ccgactgggc gtgatacggc gcccttcgaa
     2761 atgttgattg ggtaaatgga aatgtcgtcg gaaactcatg gcagcggcat aggcaataaa
     2821 gttatgttag tgggaatcga caacaaccac gtcatcgaga aacggttaca agcggcaggc
     2881 tgtgaagtaa tcaaagtgac tgatgaagag gcggcgctcg atcatgtgcg ccatgaaatc
     2941 ttcgataaag cggtgatggt ttcgacaggt tctttgattg acac
//
LOCUS       DASUTG010000470         8035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_812904, whole genome shotgun sequence.
ACCESSION   DASUTG010000470 DASUTG010000000
VERSION     DASUTG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812904"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VF089_10685"
     CDS             <1..270
                     /locus_tag="VF089_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131981.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX6174473.1"
                     /translation="LTIDQIDLVEINEAFASQVLACARELGIDLNKLNVNGGAIALGH
                     PLGCSGARIMTTLVHEMKRRGTRYGLATMCIGVGQGIATIVERIN"
     gene            374..3037
                     /locus_tag="VF089_10690"
     CDS             374..3037
                     /locus_tag="VF089_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX6174474.1"
                     /translation="MRLSKKSISLAVGTVILAAIALVYCVNLLVASRRDLVYEELQRV
                     SGKDVAFDELEVTLLTGLGFSAKQFRIADSPVFAATPLIRAKELRMGVSLLSLMLGRI
                     VINKLTFIEPEFQIITNEEGVMNLSSLEDRRKQLVDLPKARSSPSKREHSSVNFVVTN
                     FRIRNGRIDFIDRSVRAPAEMQIKRVDMNLRGINPNGRAKMTLAASLSEGLDQDVRVA
                     GYVGPFVRGKGWKEQPVEVNVEFDSLFVPLLTRAVPFFRDKIPPELDIAGPLSVRAKL
                     AGVLARPKILDFSVKAPILGSSDYNAVLDGSADLSTTGVWSEAQLSGNLHLSSIDITR
                     LRGTHMFQDLLPIALSGEGEISIESVIEGSWNRLRLGTIVRARNSELRYGTWLRKPRG
                     TAAEWSSRISRQENRFVIHESLLTLGSSRINLSGVYEHGSRARLQLRARSDRSDLSEV
                     IELFPAVQGFRATGNLHGEVLASKLLIGNSAWNMNGKLEISEAELSHETSSRKLERLS
                     ASLLLLGKQARIEDAAFRVRSSDIEMKGAVADLAIPALNLELSSANLHLVDLADFPVA
                     KSDRLQNVRLSGKVTLENGTPWLRGMVSSPDGMLQDLPYLDLRSEIIWSAADISLRDF
                     SFRALNGTWRSRDVSITETGKSQRLELYSQLKAVDLKTLLASKLPAFKDRIEGQLNFQ
                     GEFSAEAHNGAMKPGSLEGSGETRVPGGTLKDFNLVALLMSRIGSSGSPISLQLSPSL
                     AELAKSKDTSFENLEALVILEQGVIRTENLQLSTANYKITAAGWIMTDQVTRWNGMLI
                     MSLRISQELLRENKSLRYLADRSGQINLPFRVEGTLGNLRVRPDTRTIAQIVRRGSLP
                     QTIEPPAIDKRQDAQEQKESLPAELEQFLSR"
     gene            3042..3314
                     /locus_tag="VF089_10695"
     CDS             3042..3314
                     /locus_tag="VF089_10695"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HEX6174475.1"
                     /translation="MTLIPIYLEICPPDIAYIKFVLESYEEVGIIRTIDRKRAVIVLL
                     AVLDFVEVARTIIKSLQQEITLVEIPPPPDLSDDWLMTELAIDPAV"
     gene            3373..5523
                     /gene="rnr"
                     /locus_tag="VF089_10700"
     CDS             3373..5523
                     /gene="rnr"
                     /locus_tag="VF089_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX6174476.1"
                     /translation="MGRKSAIAATESSQVREKISDLFKGKPRLRLTPVEISRRAGFKQ
                     DDLQAVIDSLRRLVREGRMVRLKKNHYGLPDSDNLMTGRVHAHPDGYGFLILDEKSAV
                     DLYLNRREMRRVMHGDRVMVRVDRNKRGALETHIVQVLERGHKRLIGTYDEIEGHAYL
                     VPMDPRIAPVIPLMRGHERPEKGKIIAAEISRYGTATSPPQAELLKVIGNPDDPEVQV
                     QSIVFRYGLSTSFPPEVHRETSSASFVVSADEIASRTDLRDLPIVTIDGERARDFDDA
                     VYVRKTDAGYELYVCIADVAYYVRPATALDQEGYSRGTSVYFPDRAIPMLPEALSNGI
                     CSLNPHEDRLTKTACIEYNSRGEVIASRFFNSVIRSRERMTYTNVRRILVDRDPATIG
                     RYADLAPQFALMEELTLLIFEKRKARGNLDFDLPEAEIILDIQGLPEDIIRAERNIAH
                     RIIEEFMIAANEAVARHLTAKELPALYRVHEGPEKESLEALAPFLLSLGYRLPLKKDK
                     IAPLEIQNLLEVARGKPEERVLNRVLLRAMKQALYQPENIGHFGLASRCYTHFTSPIR
                     RYPDLIVHRMLDKALRGEKLTGAAREDLSSYLVEAGKHTSDRERNAMDAEREMVALKK
                     TQFMMDKIGEEYSGFITSLTNFGFFVELDSYFVEGLVKIGSLTDDEYYYYEKEYLIKG
                     LRHGRKFRLGDNVRVKVARINAFRSEIDFELIHS"
     gene            complement(5514..6728)
                     /locus_tag="VF089_10705"
     CDS             complement(5514..6728)
                     /locus_tag="VF089_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174477.1"
                     /translation="MLFMTLRIALRALTRNKLRAFLTMLGIIIGVGAVIAMVAIGEGA
                     KATIRSQIASLGTNVLIILPGTSVQGGVRTGSGGVNTLVDGDAKAMMRELPAVAFASP
                     ALRRNEQIVARNLNWFTLTQGVGPEFQQIRDWQVAEGRFIHEGDIESAAKVAVVGETV
                     VNQLFGNDDPIDAVIRIRNIPFRVVGVLAPKGQTSQGTDQDDTIIIPYTTMQKRLMRI
                     TFVQSIVASAISAERLPEAQAQITALLRQRHRIGPDRDDDFYIRNLSDIAEAASSSAR
                     VMAVLLGTVASISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARRKDIMLQFLVEAV
                     VMAATGGCIGILLGIGSSEILNDWAQWPTDISAPIIAIAFFFSGAVGVFFGFYPARKA
                     ASLDPIDALRYE"
     gene            complement(6732..7445)
                     /locus_tag="VF089_10710"
     CDS             complement(6732..7445)
                     /locus_tag="VF089_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174478.1"
                     /translation="MTELLRVKDLVKNYRMGDVEVPALRGVTLAVGRGEFVAVMGSSG
                     SGKSTFMNILGCLDRPTSGKYFLDGDEVGGLTADQWAEIRNQRIGFVFQGFNLLARTS
                     ALENVELPMMYSGCSAKQRHNRALDVLGLVGLGERLHHTPSQLSGGQQQRVAIARALV
                     NRPSLILADEPTGNLDSATSGEIMGLFQRLNRDQGITIVLVTHETDIAEYSKRQIHFK
                     DGLVVLDHLAGSLLSSTEH"
     gene            complement(7442..>8035)
                     /locus_tag="VF089_10715"
     CDS             complement(7442..>8035)
                     /locus_tag="VF089_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6174479.1"
                     /translation="NVKEGQEVTFTVDAFPSRRFRGRVHQVRNAPIVVQNVVTYVAVV
                     RIDNKELLLKPGMTANVQFLVSRKEDVLTIPNMAMRFKPPEEKEEAQELLRREQSRTA
                     PKLGARRTSRQSTAQGSGASGGGTRRARIYLLKDAKAQPVEIQVGITDGSRTEIRSGD
                     VQENDPVIIGMSSNADASRPALPNPFQPQRSRGVGLR"
BASE COUNT         1897 a         2238 c         2149 g         1751 t
ORIGIN      
        1 ctcactatag atcagatcga tttagtcgag atcaacgaag ccttcgcttc acaggtgctc
       61 gcctgcgcgc gcgagctcgg cattgactta aacaagctaa acgtcaacgg cggcgctatc
      121 gctctcggac atcctctcgg atgtagcggc gcgcgcatca tgaccacgct ggttcacgag
      181 atgaagagac gcggcacccg gtatggcctc gcgaccatgt gcatcggcgt cggtcaaggt
      241 atcgcgacga ttgtggagcg gatcaactga ttgcctgcca agcttgcagg caatcaggtc
      301 gggcgcggcc agcctagaaa gctcctgaac accactgctg catttgagag ttccggttca
      361 tctctcgcgg cgcatgagat tatcaaaaaa atccatttcg ctcgccgtcg gcacggtgat
      421 tctagccgcc attgcgctgg tgtactgtgt caatcttctc gtcgctagca gacgcgatct
      481 cgtctatgag gaactccaaa gggtttccgg caaagacgtc gccttcgacg aattggaagt
      541 taccttactg acggggctcg gtttttccgc taaacagttc cgaattgccg atagtcccgt
      601 gtttgccgca actccattga tccgagccaa ggaactcagg atgggtgtca gcctgctctc
      661 gctgatgctg ggcaggattg tcatcaacaa gttaaccttc atcgaaccgg aatttcagat
      721 catcaccaac gaagaaggtg taatgaacct atcatccctg gaagaccggc ggaaacagct
      781 ggtggacttg cccaaagcac gctcttctcc gtcgaaaaga gagcacagtt cagtcaattt
      841 cgtcgtcaca aacttccgaa taagaaatgg tcgcatcgac ttcatcgatc gttcagtgcg
      901 cgcgccggcg gaaatgcaga tcaagcgggt cgacatgaat ctccgcggaa tcaaccccaa
      961 tggtcgagcg aaaatgactc tggccgcttc gttaagcgaa ggtttggatc aagatgtgcg
     1021 agtagccgga tacgtaggac cattcgttcg gggcaaagga tggaaagaac agccggtcga
     1081 agtcaatgtg gagttcgact cattattcgt gccgctactc acccgcgccg ttccgttctt
     1141 tagagacaaa atcccgccgg agctcgatat cgccgggccg ctatcggtac gcgcaaagct
     1201 agccggggtt ctcgctcgcc cgaaaatttt agactttagc gtcaaagcgc cgattttggg
     1261 ttcttccgac tataacgcag tcctggacgg ctcggcggat ctttcgacaa ccggggtctg
     1321 gtccgaagct caactgagcg gcaatctcca tctgagttcc atcgatatca cccgccttcg
     1381 aggtacgcac atgtttcagg acctcctgcc tattgctcta agcggcgaag gtgagatcag
     1441 catcgagagt gtcatcgaag gatcatggaa taggttgcgt ttgggaacga tagtgcgagc
     1501 ccggaacagt gagcttcgct atggcacttg gctgcgcaag ccacggggga ccgccgctga
     1561 atggtcatca aggatttcac ggcaggagaa ccgatttgtt atccatgaat cgcttctaac
     1621 gctgggcagc agcagaataa acctttcagg cgtttatgag catggatccc gcgctcgcct
     1681 acagctcaga gcccgcagcg accggtccga tttgtccgag gtaatcgagc tgtttccagc
     1741 cgtgcaaggg tttcgagcca ccggaaactt acatggcgag gttctcgctt cgaaattgtt
     1801 gatcggcaac tccgcctgga atatgaacgg caagctcgaa atcagtgaag cggagctaag
     1861 ccatgaaacc agcagccgta aactcgagcg gctcagtgcc agcctcttgt tacttggcaa
     1921 gcaagctcgc attgaagatg ccgcgtttcg ggtcaggtca tcggacatcg agatgaaagg
     1981 agcagtggct gacctggcga ttcccgcgct aaacctcgag cttagctcag cgaatttaca
     2041 tctggtcgat cttgccgatt ttccagtggc gaaatcggat cggctgcaaa acgtcagatt
     2101 gagcggcaag gtcacattag agaacggcac gccatggttg cgaggcatgg tttcatcgcc
     2161 ggatggcatg ctgcaagatc tcccttatct cgacctgcgg agcgagataa tctggtccgc
     2221 agcagatata agccttagag atttttcttt tcgggcttta aacgggacct ggcgcagccg
     2281 ggatgtgtcg atcaccgaaa ccggcaaatc tcagcggctg gaactttact ctcaattgaa
     2341 ggcggtggat ttgaagacct tactcgcaag caaactcccc gcgtttaaag accgcataga
     2401 gggtcagctg aatttccaag gagagttcag tgccgaggcg cacaacggcg ctatgaagcc
     2461 cggctcttta gaaggctccg gcgagacccg agttcccggc ggaactctca aggattttaa
     2521 ccttgtcgcg ttgctgatgt ctcgaatcgg cagttctggg tcgccgatat cgcttcagct
     2581 ctcgcccagt ttagccgagt tggcgaaatc caaagacacc tcattcgaaa atctcgaggc
     2641 gctcgtcata ctcgagcaag gagtgattcg caccgagaac ttacagcttt caaccgcaaa
     2701 ctataaaatc acggcagcgg gatggattat gaccgaccag gtaacccgat ggaatgggat
     2761 gttgatcatg tcactgcgaa tcagtcagga gctcttacgc gagaataaaa gcctgcgtta
     2821 cctagcagac cggagcggcc aaatcaattt gccattccgc gtcgaaggca ctctgggaaa
     2881 tttgcgagtt cggccggaca cccgcactat cgcgcagatc gtccggcgcg gatctctgcc
     2941 gcaaacaatc gaaccaccgg caatcgacaa acgacaggac gcgcaagaac aaaaagaatc
     3001 gctaccggca gaactcgagc aatttttgag tcgctaagtc catgaccttg atccccatct
     3061 acttagaaat ttgccctccc gatatcgcat acatcaaatt tgttttggag tcctatgaag
     3121 aagtcggcat cattcgcacg atcgatcgca agagggcggt cattgtgctt ctagccgttt
     3181 tagattttgt cgaggttgcc cggacgataa taaaatcact gcagcaggaa atcactttgg
     3241 tcgaaatccc accaccgcca gacctgagcg atgattggct aatgacggag cttgcgattg
     3301 atccagcagt atgatcatca cggccgccag aactgattgc aagccatcgg gttccatcag
     3361 atcgtaactt gtatggggcg taaatccgca atcgcagcga cagaatcgtc gcaggtacga
     3421 gaaaaaatct ccgacctttt caagggaaag ccgcgccttc ggttgacccc ggtggaaatc
     3481 agccgaaggg ccggcttcaa acaggacgac ctccaagctg tcatcgattc cctgcgccgg
     3541 ctggtgcgcg aaggaaggat ggtccggcta aaaaagaatc actacggttt gcccgatagc
     3601 gacaacctga tgacgggcag agtccacgcc catccggacg gttacggatt tcttatcctc
     3661 gacgaaaaga gcgctgtgga tctctactta aatcggcgcg agatgcgtcg tgtgatgcat
     3721 ggcgatcgcg ttatggtacg agttgatcgg aacaaacgcg gcgccttgga aactcacatc
     3781 gtgcaggtac tggaaagggg tcacaagcgt ctcatcggaa cttacgatga aatcgaaggt
     3841 cacgcctatt tagtcccgat ggatccgaga atcgccccgg tgatcccgtt aatgcgcggt
     3901 catgaacggc cagagaaggg caaaatcatt gctgctgaga tttcccgcta tgggaccgcg
     3961 acctcgccgc cacaagccga gctcctgaaa gtaatcggca acccggatga tcccgaggtg
     4021 caggttcaat cgatcgtttt ccgttatggg ctgagcacca gctttccgcc cgaggttcac
     4081 cgagagacaa gcagcgcctc cttcgttgtc agtgccgatg aaatcgcctc ccgtaccgac
     4141 ctgcgcgatt tgccgatcgt cacaatcgat ggcgagcgag cacgcgactt tgatgatgcc
     4201 gtttacgttc gcaagaccga cgcagggtac gagctctacg tttgcattgc tgatgtcgcg
     4261 tattatgtca gaccggccac ggctctggat caggaaggtt acagccgggg caccagcgtt
     4321 tattttcccg atcgtgccat ccccatgctc cctgaggcgc tctccaacgg catctgcagt
     4381 ctcaatcccc atgaggaccg gctgacgaaa accgcgtgca ttgaatacaa ttccaggggc
     4441 gaagtcatcg ccagccgctt ctttaactcg gtgatccgca gccgagagcg catgacctat
     4501 accaacgtgc gccgcatcct ggtagatcgc gatcccgcga caatcggacg ctatgcggat
     4561 ctggcgcctc aattcgcatt gatggaagag ctcacgttgc tgattttcga aaaacgcaaa
     4621 gctcgtggca atctagattt cgatctaccg gaagcggaga tcattctcga cattcaagga
     4681 ttgccggaag acatcatccg cgccgagcgc aacattgcgc atcgcatcat tgaggaattc
     4741 atgatcgccg ccaacgaggc agtggcgcgt catttgactg caaaggaatt accagcgctt
     4801 tatcgcgtgc acgagggacc ggagaaagaa tctcttgaag ctctagcccc attcttactg
     4861 agcctgggat accggctgcc gctgaagaaa gacaagatcg cgcctctaga aatacaaaac
     4921 ctgctcgagg ttgcccgcgg taagcccgag gagagagtgc tcaatcgtgt tcttttacga
     4981 gcgatgaaac aagcgctata ccaacccgaa aacatcggcc acttcggact ggcctcccgg
     5041 tgctacactc attttacttc gccgatccgg cgctatcccg atttgatcgt gcaccgaatg
     5101 ctcgacaaag cgcttcgagg ggagaaattg acaggcgccg cgcgtgaaga tctctcgtcc
     5161 tatctcgtag aagccggcaa gcacacatcc gacagggagc gcaatgccat ggacgccgaa
     5221 cgagagatgg tcgccctgaa gaaaacccaa ttcatgatgg ataagatcgg tgaggagtac
     5281 agcggcttta tcaccagctt gacgaacttc ggattctttg tcgagctcga cagctatttc
     5341 gttgaagggc tggtgaaaat cggctccctg accgatgacg agtactacta ttacgaaaaa
     5401 gaatacctga ttaaaggcct gcgtcacgga cggaagttcc gcttgggcga caatgtccgg
     5461 gtcaaagtcg cgcgcattaa tgcgtttcgc agcgagattg attttgaatt aattcactcg
     5521 tagcgcagcg cgtcgatggg atctagactg gcggcttttc tcgcgggata gaaaccgaag
     5581 aacactccga cagcgccgga aaagaaaaag gcgatggcaa tgatcggtgc gctgatgtca
     5641 gtgggccact gcgcccagtc attgaggatt tcggagctac cgatacccaa caatatgccg
     5701 atacagcctc cggttgcggc cataaccacg gcctcgacca agaactgcag catgatgtct
     5761 ttgcgccggg ctcccaccgc catgcgaatg ccgatttcac gagtgcgctc ggtcacggaa
     5821 accagcatga tattcatgat accgatccct ccgaccaata gagagatcga agccacagtg
     5881 ccgagcagca cagccatgac tcgcgcgctg cttgaggcgg cctcggctat gtccgagaga
     5941 ttacgaatgt aaaaatcgtc gtcgcgatcg ggccctatgc ggtgtcgctg gcgcaagagc
     6001 gcggtgatct gtgcttgcgc ttccgggagg cgttcggcac tgatggccga agcaacgatg
     6061 ctttgcacga aggtgatacg catcaagcgt ttttgcatcg tggtataggg aatgatgatg
     6121 gtgtcatctt gatcagtccc ttggctggtt tgtcctttcg gcgcaagcac gccgacaacc
     6181 cgaaacggaa tatttctaat gcgaatgact gcatcgatgg gatcgtcatt accgaacaac
     6241 tgattaacga cggtttcgcc gacaaccgcg actttggcgg cactctctat atcgccctcg
     6301 tgaataaatc gtccttcggc aacctgccaa tcgcgtatct gctggaattc cggcccgacg
     6361 ccttgtgtta gggtaaacca gttaagattg cgtgcgacga tctgctcgtt gcggcgtaac
     6421 gcaggcgagg cgaacgcaac agcaggcagc tctcgcatca tcgccttggc atcgccatca
     6481 acaagagtat tgacacctcc agaacccgtt cgtaccccgc cctgcacaga cgtgccgggc
     6541 aatataatca aaacgttggt tcccaaactg gcgatttggg atcgaatcgt cgccttggct
     6601 ccttcgccga tcgccaccat tgcgatgact gcgcctacgc caatgatgat gccaagcatc
     6661 gtcagaaaag cgcgcaattt gtttctcgtt agagcacgga gcgcgatccg cagcgtcatg
     6721 aacaacattg ctcagtgctc cgttgatgac agaagcgatc cggctaggtg gtccaaaacc
     6781 accaagccat ctttaaaatg aatctgacgc ttggagtatt cggcaatgtc ggtttcatga
     6841 gttactaaaa cgatggtaat cccctggtca cgattaagtc tctgaaataa ccccatgatc
     6901 tccccgctgg tggcggaatc gagattccca gtgggttcgt cggcgagaat caacgatggc
     6961 cggttcacga gtgctctggc aatcgccacc cgctgctgct ggccgccgga aagttggcta
     7021 ggcgtatgat gaagacgctc accaagaccc accagaccga gcacatccag cgcccggtta
     7081 tgcctttgtt tggcactaca gccactgtac atcatcggta gttcaacgtt ctccaatgcg
     7141 ctggtgcgcg caagcaaatt aaagccctga aacacgaaac cgatacgctg gttgcgaatt
     7201 tccgcccatt gatccgcagt caatccccca acctcatcgc catcaagaaa gtacttcccg
     7261 ctggtcggcc gatcgagaca gcccagtata ttcataaagg tcgacttgcc cgagccggaa
     7321 gaacccatta ccgctacgaa ctctccacgc ccgacggcca gagtgacgcc gcgcagcgcc
     7381 ggcacctcca catcgcccat ccggtagttc ttaacgagat ctttgacccg gaggagctcg
     7441 gtcatcgcaa tccgacacca cgcgaccgct gcggctgaaa tgggttcggc agcgccggac
     7501 ggctagcatc cgcattgctg ctcatgccga tgatcaccgg atcgttttct tgcacatcac
     7561 cgcttctgat ttcggttctc gatccatcgg tgatgcctac ctgaatttcg accggttgag
     7621 ctttagcatc cttaaggaga tagatacgcg cgcgcctcgt tcccccgccc gaagcgccgc
     7681 tcccttgcgc cgtgctttga cggctggtcc gcctcgctcc taattttggc gcggttcggc
     7741 tctgttcgcg gcgcagaagt tcctgcgctt cttctttctc ttcaggcggt ttgaagcgca
     7801 tagccatatt cgggatggtc aggacatctt cttttcggct gaccagaaat tgcacattgg
     7861 cggtcatacc cggcttgagc agcaactctt tgttgtcgat gcggacaacg gccacatacg
     7921 tcacgacatt ttgcacgacg ataggtgcat tgcgcacttg atgcacgcgc ccccgaaagc
     7981 ggcgcgaggg gaaggcgtcg acggtaaacg tgacttcctg cccttctttg acgtt
//
LOCUS       DASUTG010000471         3575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1554187, whole genome shotgun sequence.
ACCESSION   DASUTG010000471 DASUTG010000000
VERSION     DASUTG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554187"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /gene="rpsC"
                     /locus_tag="VF089_10720"
     CDS             complement(<1..307)
                     /gene="rpsC"
                     /locus_tag="VF089_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6174480.1"
                     /translation="MGQKTHPKMFRLGVIESWDSKWYSRHDYAKLLHEDFRVKKFLKS
                     RLYHAGISKIEIERAANKAKINIHTARPGIVIGKKGAEIEKLKEEIAKLTNRETYINI
                     "
     gene            complement(309..653)
                     /gene="rplV"
                     /locus_tag="VF089_10725"
     CDS             complement(309..653)
                     /gene="rplV"
                     /locus_tag="VF089_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6174481.1"
                     /translation="MEARAITKFIRLSPRKVRLVVDQIRGKGVEEALNILKFTPKRSA
                     LVVSKTLRTAVANAESTQSVDVDQLYVKQVKVDEAGMWKRFTPRAQGRATRIRKRLSH
                     VTIVLDEVSERK"
     gene            complement(718..1026)
                     /gene="rpsS"
                     /locus_tag="VF089_10730"
     CDS             complement(718..1026)
                     /gene="rpsS"
                     /locus_tag="VF089_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6174482.1"
                     /translation="MPRSIKKGPFVDEHLTKKISAMNESRQKQVIRTWSRRSTVTPEM
                     VGHTIGVHNGRKFIPVFISENMVGHKLGEFSPTRTFFAHSGDKKGESGQGGPRPAGGR
                     "
     gene            complement(1029..1856)
                     /gene="rplB"
                     /locus_tag="VF089_10735"
     CDS             complement(1029..1856)
                     /gene="rplB"
                     /locus_tag="VF089_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6174483.1"
                     /translation="MAIRTYRPTSAGIRFRTGLTFDEIAKKRPEKALRTPLGHSGGRN
                     NNGRITADHRGGGHKRLYRVIDFKRNKKGVPAKVEAIEYDPNRSANIALLCYADGERR
                     YILAPERLKVGDSVIAGDDNIDIQPGNSLPLKFVPVGTVVHNVELKIGKGGQLARSAG
                     SGIQLMAKEGSYALLKLPSGELRNVLADCRATIGQVGNADQENVSWGKAGRMRWLGFR
                     STTRGIAKNPVDHPHGGGEGRSKGNHPMTPWGKPTKGYKTRKNHSTDKYIIKGRKAK"
     gene            complement(1870..2160)
                     /gene="rplW"
                     /locus_tag="VF089_10740"
     CDS             complement(1870..2160)
                     /gene="rplW"
                     /locus_tag="VF089_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6174484.1"
                     /translation="MRGPQEIIKAPVISEKGSLLAETVQQVLFKVRPDANKVEVKKAV
                     ESLFKVKVVKVRMARYLGKVRRVGRSTGRRPEWKKAYVTLREGDKIDFFGAA"
     gene            complement(2157..2753)
                     /gene="rplD"
                     /locus_tag="VF089_10745"
     CDS             complement(2157..2753)
                     /gene="rplD"
                     /locus_tag="VF089_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6174485.1"
                     /translation="MEVHVAQPKLDERIFGVNARPHLLHQAVVMQLRNRRAGTAATKS
                     KGFVRGGGKKPWRQKGTGRARAGSIRSPIWVGGGTIFGPQPRDYSFRMPRKARREALL
                     SALSLKNQEGKLIVLDKLELEEAKTKLMASALAELEVQSALLVVSQGDVKIERASRNL
                     PKIKVLRVEGLNVYDLLRYDHLILMEGALKLLEERLAS"
     gene            complement(2760..3392)
                     /gene="rplC"
                     /locus_tag="VF089_10750"
     CDS             complement(2760..3392)
                     /gene="rplC"
                     /locus_tag="VF089_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6174486.1"
                     /translation="MTGLIGKKLGMTQFYSADGNVVPVTVIETGPCVVVQKKETAIDG
                     YNALQVGFGGKKSQRVNKAIQGHMAKAGKGAFSILREFRSDDVTGYQVGQEIKVADLF
                     KAGDLIDVSGVSKGHGFWGVIKRWSFAGFPGSHGTHEYFRHGGSIGNRSYPGRVRKGK
                     KMAGHWGNENISIQNLKVIDVRAEENLLLVKGAVPGAKQGIVILRRAVKG"
     gene            complement(3436..>3575)
                     /locus_tag="VF089_10755"
     CDS             complement(3436..>3575)
                     /locus_tag="VF089_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6174487.1"
                     /translation="KISRDQFEIRTHKRLLDILEPTQQTIDALGKLDIAAGVDVEIKL
                     Q"
BASE COUNT          776 a         1036 c          813 g          950 t
ORIGIN      
        1 gaatgttgat gtaggtttcg cggttggtga gtttggcaat ttcttctttt aacttttcga
       61 tctctgcgcc ttttttaccg attacaatcc cgggccgcgc ggtatgaata ttgattttcg
      121 ccttattagc ggcccgttca atttcaattt tcgagatgcc ggcatgatag agtcggcttt
      181 ttagaaactt cttcacgcgg aaatcttcgt gaagcaattt ggcataatcg tggcgggagt
      241 accacttcga atcccatgac tcaattacac ctaaacgaaa catctttgga tgtgttttct
      301 gacccatgct atttcctctc actcacctca tccagcacga tggtgacatg actcaagcgc
      361 ttacggattc gcgtggctct tccctgcgct ctcggcgtaa atcgcttcca cattccggcc
      421 tcgtcgactt taacctgttt gacatagagc tgatccacat cgacgctctg cgtgctttcg
      481 gcgttggcga cagccgtgcg caaagtcttg gacacaacca gtgcggaacg cttcggtgta
      541 aattttaaaa tgttcagcgc ctcttcaacc cctttgccgc gaatctgatc gactaccagc
      601 cgaactttgc ggggagataa cctgataaat ttcgtaatcg cgcgagcttc cataactcag
      661 ttaaagacct ttgattcgat gcatgttgaa ttcttggcca ctatttgcca gccggtacta
      721 tctacccccg gcgggcctcg gtccgccctg acccgattca ccctttttgt ctcccgaatg
      781 agcaaagaag gttcgcgtag gagagaactc accgagttta tgtccgacca tgttttcgga
      841 gataaacacc ggaatgaact ttcgaccgtt gtgcacgccg atggtatgac cgaccatctc
      901 cggggtgacc gtagatcggc gcgaccaggt tcggatgact tgcttctgac gtgactcgtt
      961 catggctgaa atcttcttgg tcaggtgctc atccacgaac ggtccctttt ttatcgaacg
     1021 cggcatgtct atttagcctt tctccccttg atgatgtact tatccgtgga atgatttttt
     1081 cgtgtcttgt agcctttggt cggcttcccc cacggtgtca tcggatgatt tcctttggaa
     1141 cgtccttcgc cgccgccatg cggatgatcg accggatttt tggcgatgcc tcgggtggtg
     1201 gacctgaaac cgagccagcg catccgtccg gccttacccc atgaaacatt ctcctggtcg
     1261 gcattgccga cctgaccaat ggttgcgcgg caatcggcga gaacgttacg gagctcacct
     1321 gaaggaagct tcagcaaagc ataagaaccc tctttggcca ttaactgaat gcctgaaccc
     1381 gcgcttcgcg ccaactgacc gcctttgcct attttaagtt cgacgttatg aaccacagta
     1441 cccactggaa cgaactttaa aggtagcgaa ttgccgggct gaatgtctat gttatcgtcg
     1501 ccagcaatca cggaatcgcc tacttttaga cgttcgggag cgagaatgta ccgccgttcg
     1561 ccatcggcgt aacaaagtag cgcaatattg gctgagcgat tcggatcata ctcgattgct
     1621 tcaaccttcg ccggaacacc cttcttatta cgtttgaaat cgattacgcg atagagccgc
     1681 ttgtgtcctc cgcctcgatg atcagcggtg atacgtccat tattgttacg gccgccgcta
     1741 tgacccagag gcgttctcaa cgctttctcc ggtcgcttct tggcgatctc atcgaaggtc
     1801 aagccggttc gaaaacgtat cccagccgat gtcggtcgat aggttctaat agccattgtg
     1861 ttcttctttc taggccgcgc cgaaaaagtc gatcttatcg ccctcgcgca atgttacgta
     1921 cgccttcttc cactccggcc gccgcccggt acttctgcca acccgccgca ctttccccaa
     1981 ataacgggcc atgcgcactt taacaacctt gaccttgaag agagactcga ccgctttttt
     2041 aacttcaact ttgttggcat cagggcgcac cttgaacaac acctgctgca ccgtttcggc
     2101 aagaagcgaa cctttctccg aaatgaccgg cgccttgatt atttcctgcg gtcctctcat
     2161 gaagccaacc tctcttccaa gagtttcaat gcgccttcca tgagaatcag atgatcgtat
     2221 cggagaagat cgtagacgtt caaaccttcc actcggagca cttttatctt tggcaagtta
     2281 cgagaggcgc gctcgatttt cacatcaccc tgtgaaacca ccagcaaggc gctttgcacc
     2341 tctaattccg caagcgcgct cgccataagc ttggtcttgg cttcctcaag ctcaagctta
     2401 tcaagaacga tgagcttacc ttcctgattc ttaaggctta acgccgatag caaagcctcg
     2461 cggcgcgctt tacgcggcat acggaaagaa taatcgcgcg gctgaggacc gaaaatcgta
     2521 ccgcctccaa cccatatcgg cgatcggata ctgcccgcgc gggcccgtcc ggtgcccttt
     2581 tgacgccacg gcttcttgcc gccgccacgg acaaaccctt tcgacttggt tgcagctgta
     2641 cccgcgcgtc ggttccgcaa ctgcatgact acagcctgat gcagcaaatg ggggcgcgca
     2701 ttgacgccaa agatccgttc atcaagcttg ggctgtgcca catgaacttc catcgcaact
     2761 tatcccttta ccgcccggcg gagaatcacg atgccttgct tggcgcctgg aacagctccc
     2821 ttgacgagga gcagattctc ttccgcgcgg acgtctataa ctttcaaatt ttggatcgat
     2881 atattctcgt tgccccaatg gccagccatt ttcttacctt tgcgcactcg ccctggataa
     2941 gatcggttac cgatcgatcc accgtgcctg aaatactcgt gagtaccatg cgatcctgga
     3001 aagccggcaa aggaccatcg cttaataacc ccccagaagc catgtccctt tgaaaccccg
     3061 gagacgtcga tcagatcccc ggccttgaac agatcagcca ctttgatctc ttgccctacc
     3121 tgatatcctg tcacatcgtc agatcggaat tctcgcaaga tcgaaaatgc acctttgccg
     3181 gctttggcca tatggccttg aatggcctta ttaacccgct ggctcttttt gccgccgaag
     3241 cctacctgca gcgcattgta accgtctatc gctgtctctt tcttctgcac tacaacacag
     3301 ggcccggtct caatgaccgt aacgggaacc acattcccgt cagcagaata aaattgcgtc
     3361 atcccaagtt tttttcctat aaggccagtc atatgattat ctcaggcgaa gcttttctag
     3421 tagaactctc tccccttatt gaagcttgat ctcaacatcc acacccgcag cgatgtcgag
     3481 ctttcccaat gcgtcaatgg tttgctgagt cggctccaaa atatcaagca atctcttatg
     3541 ggttcgaatc tcaaattggt cgcgggatat tttgt
//
LOCUS       DASUTG010000472         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_665999, whole genome shotgun sequence.
ACCESSION   DASUTG010000472 DASUTG010000000
VERSION     DASUTG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665999"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VF089_10760"
     CDS             <1..185
                     /locus_tag="VF089_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEX6174488.1"
                     /translation="IGSSLQVQPAASFPVIAKHNGALLAIVNRDVTPLDDIADYVYHG
                     GIGEWFEQLSPLLSDA"
     gene            931..1338
                     /locus_tag="VF089_10765"
     CDS             931..1338
                     /locus_tag="VF089_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6174489.1"
                     /translation="MQSLSTIEGGCLCGTVRYRASGKAYGITHCHCRTCRRASGAPLV
                     TWAAFDSDKFNFTQGQPVSYASSKNVVRTFCGRCGTALTYRRLDLPGSVDLTLGSMDD
                     PEQLQPQDHTWTESRISWLHLGDDLPVYPQDRR"
     gene            1571..2053
                     /locus_tag="VF089_10770"
     CDS             1571..2053
                     /locus_tag="VF089_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015221205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174490.1"
                     /translation="MADAKRFILTSSEIRQREQPFSHPWNPKSRLMGVQLSRALGLKR
                     TGVSIARIPPGKESFEYHSHQSEEEWLYILSGRGIAEIDGEEIEIAAGAFMAFPTPSV
                     PHHLKNPFDQDLIYLMGGENHEIEIADFPRLGKRMIRSREKIDIYEVSDAKPFGPLEP
                     "
     gene            2228..2431
                     /locus_tag="VF089_10775"
     CDS             2228..2431
                     /locus_tag="VF089_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174491.1"
                     /translation="MKSILLWLFVINLGIAFGAGLYEARVVIPQWANLSPKDWPNTGL
                     LFWVYVTTVPLTLLALANSSLLG"
BASE COUNT          591 a          668 c          678 g          633 t
ORIGIN      
        1 tcatcggttc gtcgctccaa gtccagcccg ccgcctcttt tcctgttatc gccaaacaca
       61 acggcgccct gctggcgatc gtcaatcgcg atgtaacacc tttggatgat attgccgact
      121 acgtttatca tggcggcatc ggcgaatggt tcgaacaatt gagcccattg ctaagcgacg
      181 cctgaagccg atatattcgg ttctaattta tgcgatcaat atttcttctt gccctcacct
      241 tgaccttggc cggttgcgca accgagcgaa ccgttctggt gaacagccaa ggtgatctac
      301 ttacctgcga aaccagtggt tcggggttct ttggtgctat ctcggtccgc aatcagcaga
      361 aacagtgcat ctccgatgcc gagacgcgcg gctatcgggt aaagaattaa cggctcgcaa
      421 tcttgcggcc tgacccgaag cctccggaga aaaagattct ccccagcgag agactgaatg
      481 cgagcaccgc ctgaagtttg cggtgcagtt gcggatccgg cagtacaatc ttaattttta
      541 aactggcagc gagaagcatg tccctcgaga cgaggggagc acgcgtcttg aaccgcctcg
      601 gaacctagat atatccttgg ttagtgaacc ggcaagtgtc gtcgcgccga tcattgagcg
      661 cttgagaatc acgagaaatg cccgtcgtga tctcgaaatc aacgagtgcc aggtcgcatt
      721 gaagtactat ttggctatgt acttactcgg gcgcaaatat gtcggcagca ggttctcccg
      781 catgccatcg atctcacgtc ttagttgcac aggctacaac cttactgtaa tcggatgaga
      841 agattcgcag gaagatcgtt atattcgatt tcattttctt gaaacctcgt ctcagccgta
      901 cacactcaag cccgttgtcg aggaattgtc atgcaatcgt tgagcacaat agagggaggt
      961 tgcctctgtg gcacggttcg gtatcgcgcg tcgggcaagg cctacgggat aacccattgc
     1021 cactgtcgga cttgccggcg cgccagcggt gcgcctttgg tgacatgggc ggcatttgat
     1081 tctgacaagt tcaacttcac acaaggccaa cctgtttctt atgcgtcgtc gaaaaacgta
     1141 gttcggactt tttgcggccg ctgcggcacc gcgctcacgt accggcgtct cgatctgccg
     1201 ggttccgtcg atttgactct aggaagcatg gacgatccag agcaactaca gccgcaggat
     1261 catacctgga ccgagagtag aatttcatgg cttcatcttg gtgatgacct gccggtttat
     1321 ccccaggaca gaaggtaatt cagagaaatt ttcgcttacc tctaaaccag gaaggacaag
     1381 catgccgaag attctgcatc aacttacgtc agcagtccgg tctcgagctc gatgaaacat
     1441 tccaattctg tgagccatgc gctagccgcc gtgcagggtc cgctggaaag gttggtttat
     1501 gaactgtaag catctgcgcc ggcacggatg acacttctct atctgctggc agaagatctg
     1561 aggtaagatt atggccgatg caaagagatt catactcacg tcttctgaga tcaggcaacg
     1621 cgagcagccg ttctcgcacc catggaaccc gaaatctcgc ttgatgggcg tccaactcag
     1681 tcgcgcgctt ggcttgaagc ggaccggcgt gagcatcgcg cgcataccac ccgggaaaga
     1741 atcgtttgaa taccattcgc accaatccga agaagagtgg ctttatatcc tttcgggacg
     1801 cggcattgct gaaatcgacg gggaggagat agaaatcgct gccggcgcat ttatggcgtt
     1861 ccccacacct tcggtcccgc atcatttgaa gaatcctttc gatcaggatc tgatttacct
     1921 gatgggtggt gaaaaccatg agatagagat tgccgatttc ccgcggttgg gcaagcgaat
     1981 gattaggagt cgtgagaaga tcgacatcta tgaggtgtcc gatgcaaagc catttggtcc
     2041 gctcgaaccc tgattccagt tcgaggtgtc taggcatccg ttcaaccgcc ttcattcgcg
     2101 ataagtaaac cagacggtgc gacacaagaa acttgactgt cctgcaagcc gaggctcatt
     2161 cgtcgtattg atgaaacgaa aataacggtt gaaagaattt tgatcttgcg ctcatggtca
     2221 gggagttttg aaatcaatcc ttctttggct cttcgttatt aacctgggga tcgcgttcgg
     2281 cgccgggctt tatgaggcgc gggtcgttat tccgcagtgg gcgaacctgt cgcctaaaga
     2341 ttggccgaat accggcctct tgttttgggt ctacgtaacg actgttccgc ttacgctgct
     2401 ggcgttggcg aactcatcac tgcttggtta gacgagggcg gaagacgcaa atggtggctc
     2461 ggtgcagtat tcattatcct gatagagcgg attacaactt cctcctattt cataccggct
     2521 atgctcacac tcaccagttc ggatttgccc catgcagaga ttgctgcaag
//
LOCUS       DASUTG010000473         3136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1554322, whole genome shotgun sequence.
ACCESSION   DASUTG010000473 DASUTG010000000
VERSION     DASUTG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554322"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF089_10780"
     CDS             <1..215
                     /locus_tag="VF089_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174492.1"
                     /translation="LIAKIGQQVYLEAHRDVYKKDSDAPRLFEELLQAFNLESVVDRQ
                     LARSIIRKEDGIARPITRGVGAMDSQ"
     gene            212..814
                     /locus_tag="VF089_10785"
     CDS             212..814
                     /locus_tag="VF089_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HEX6174493.1"
                     /translation="MTKFHQSLASSYQTADAKPMRMTRRTFVKLGTMAAITGLLPSAV
                     RGSSKNASMGEKSLAFYNTHTGERLKAVYWAEGGYVESSLREISYILRDPRNNEIHEI
                     DNRLLDLLFSVRKEIETNEPFHVISGYRSARTNAFLRAHSTGVAENSLHLVGQAIDIR
                     LPGRETRILQKAAIALKGGGVGYYPKSDFVHVDIGRVRYW"
     gene            complement(994..1422)
                     /locus_tag="VF089_10790"
     CDS             complement(994..1422)
                     /locus_tag="VF089_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6174494.1"
                     /translation="MAIFKTILAPTDFSDVSAAGVRYACQLAKDVGAEIIIFNVDVLD
                     ESNRIDKREIEQHKKRLGKFVAEKVADACVGLKMRQVVDAGQPFGAIVDCAEKEGVDL
                     IVMSSHGRSGLSRMLIGSVTDKVLRGSPCPVLVVPLKKEN"
     gene            complement(1505..1990)
                     /locus_tag="VF089_10795"
     CDS             complement(1505..1990)
                     /locus_tag="VF089_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEX6174495.1"
                     /translation="MQFSTVRIQKPEDVNIILGQSHFIKTVEDIHEALVQTVPGIKFG
                     LAFCEASGAMQVRWSGTDEAMLELAKCTALALSAGHSFIIFLGPGFFPVNVLNAIKNV
                     PEVCGIFCATANPVEIVIAETEQGRGIMGVIDGGKCQAVEDEEGIARRKQLLRTIGYK
                     L"
     gene            complement(2028..2891)
                     /locus_tag="VF089_10800"
     CDS             complement(2028..2891)
                     /locus_tag="VF089_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6174496.1"
                     /translation="MYSKILVPLDGSHVSEKILPYARFFADAYGIPVELLRIADPDAR
                     PLFWPSETSGQYLKKIADENFSGSATTAAEVGAPATVIVDRAKTDPSCLIAMATHGIS
                     GARRWLLGSVTSKVVQTATNPLLLIRPTDTPDALRPVSMDQIFVPLDGSGLAEKTLPH
                     ACALTKRLNLEIQLLRVYWLPMNAYVVADGVIAQGPGQYREELARQAQDYLDGKIAGL
                     RADGFKRVVAVALEGDAASEIIDLAGKAPNSLIAMSTHGRSGIGRWVLGSVAEKIIQH
                     AHNPVLLIRAN"
     gene            complement(2898..>3136)
                     /locus_tag="VF089_10805"
     CDS             complement(2898..>3136)
                     /locus_tag="VF089_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6174497.1"
                     /translation="MKQQGLHRISFAAKKGSEADEILAIARQIPDTMIAMCTHGRSGV
                     QRWALGSVTERVVRHSDSPVLVVRAGLDKGSAGK"
BASE COUNT          734 a          903 c          808 g          691 t
ORIGIN      
        1 tcctgatcgc caaaatcggg cagcaagttt acttagaagc ccatcgagat gtttacaaaa
       61 aagattcaga tgccccacga cttttcgaag aattgctgca agccttcaat cttgaatctg
      121 tcgttgaccg tcagctggca cgcagtataa tccgcaaaga agacggcatc gctagaccga
      181 ttacacgcgg agttggagct atggattccc aatgacaaaa ttccaccagt cgctggcaag
      241 cagctatcag accgccgacg ctaagccgat gcgaatgacc cggcgaacgt tcgtcaagct
      301 cggcaccatg gcggcaatca ccggattatt gccgtccgcg gtccgcggct caagtaaaaa
      361 cgcgtcaatg ggagaaaaat ctctggcgtt ttacaacacg catacgggcg agagactcaa
      421 ggccgtttac tgggcggaag ggggttacgt agaaagctcc cttcgcgaga tcagctacat
      481 cttacgcgat ccgcgcaaca acgaaattca tgagatcgat aaccggctat tggatctcct
      541 cttcagcgtc cggaaagaga ttgaaaccaa cgagccgttt cacgtcatct ccggctaccg
      601 ttccgcacgt acgaacgcgt tcctgcgcgc acacagcacg ggagtggcgg aaaacagctt
      661 gcatctagtc ggccaagcga tcgatatccg attacccggc cgagaaacgc gcatcttgca
      721 aaaggcagcg atagctctaa agggcggcgg cgtcgggtat tatcccaagt ctgactttgt
      781 ccacgtcgat attggacgtg tgcgatactg gtgaagggga ttaagagtaa gaaaagacgt
      841 tcggcatcga ttcgatccgc gccgctgtcg aacttcatac ggcattctcg cctaccaatg
      901 tgcaactgga gcaatactag cgcaaccctc cagcgcaggt tcgaatctcc attgaccatt
      961 tgaatggaac agcagattgc cgcgccggag ttgctaattt tcttttttta acggcacgac
     1021 caggaccgga cagggcgatc cgcgtagaac cttgtccgtg acgctgccga tcaacatgcg
     1081 cgataagccg ctgcgtccgt gcgaggacat gacgatcaag tcgacgcctt ccttctcggc
     1141 gcagtcgacg atggcgccga agggctggcc agcatcgacc acttggcgca ttttcaatcc
     1201 gacacaagca tcggcaactt tctcagcgac aaacttcccg agcctttttt tgtgttgttc
     1261 gatctcgcgc ttatcgatgc ggttgctctc gtccaataca tccacattga agatgataat
     1321 ctcagcccct acatctttag ccaattggca ggcgtagcga acgccggcgg ctgagacgtc
     1381 ggagaagtcc gttggtgcaa gtatcgtttt aaagattgcc atacgagctc cttgtcgggc
     1441 cgcgagaaca gtcgatcaaa tgtatcttcc cgttggcgag aaccattatt acgtcttctc
     1501 gcaatcacaa cttgtaacca attgtgcgca gcagttgttt gcgccgcgca ataccctctt
     1561 catcttcgac cgcctggcat ttgccgccgt cgatgacgcc cataataccg cggccttgtt
     1621 ctgtctctgc gatcacgatt tcgaccggat tcgccgtggc gcagaaaatt ccacaaactt
     1681 cgggaacatt cttgatcgca ttgagcacgt ttaccggaaa gaagcctggc ccgagaaata
     1741 taatgaagct gtgtccggcg gaaagcgcta atgcggtaca tttcgcaagc tcgagcatgg
     1801 cctcatctgt gccactccaa cgaacctgca tcgcgcccga cgcttcacag aacgctaagc
     1861 cgaatttgat tccaggtacg gtttgcacca gagcctcgtg aatgtcttca accgtcttga
     1921 tgaaatggct ctgacccaga ataatattga cgtcctccgg tttctgtatt ctaacggttg
     1981 agaattgcat gggattgcct tagcagccgc aagtcgctca ctgactttca attcgcgcga
     2041 atcagcagta ccggattgtg cgcatgctga atgatcttct ccgcaacact ccccagaacc
     2101 caccggccaa tcccggaccg cccgtgcgtc gacattgcaa ttaaactatt tggtgctttg
     2161 ccggccaaat cgatgatttc gctcgcggcg tccccttcaa gcgcgacagc aacgacccgc
     2221 ttgaacccgt cggctctgag gcccgcaatt ttgccgtcga gataatcctg cgcctgtctg
     2281 gccaattcct cgcgatactg ccccggaccc tgagcgatca cgccgtcggc aacgacatag
     2341 gcgttcatgg gtaaccagta aacacgcagc aattgaatct caagattcag tctcttcgtg
     2401 agtgcacatg catgcggcag ggttttttcc gccaaaccgg atccatcgag cgggacaaag
     2461 atctgatcca tgctgaccgg cctcaaagca tcgggagtat cggtcgggcg aatgagcagc
     2521 agaggattcg tcgccgtctg aacgactttg ctcgtcacgc taccgaggag ccaacgtcgc
     2581 gctccagaga tgccgtgtgt cgccatggcg atcaggcagg aaggatcggt tttggcacga
     2641 tcgacaataa cggttgccgg cgcgcccacc tcggcggcgg ttgtagccga gccggagaaa
     2701 ttttcgtcgg caattttctt tagatactga ccgctagttt cggagggcca aaaaagtggg
     2761 cgcgcgtctg gatccgcgat gcgcaacaac tccacgggga tgccataggc atcggcaaag
     2821 aaacgggcat agggcagaat cttctcggaa acatgcgatc catccaaagg cacgagaatt
     2881 ttgctgtaca tggctcatca ctttcccgcg gatcctttat caagccccgc gcgcacgacc
     2941 aaaaccgggc tatcggaatg gcggacgacc ctctcggtaa cgctgcctag cgcccagcgc
     3001 tgcacgccgg aacggccatg cgtgcacata gcgatcatcg tatccgggat ctgccgcgct
     3061 atcgctagta tttcgtctgc ctccgatccc ttcttcgctg cgaaggagat cctatggaga
     3121 ccctgctgct tcatct
//
LOCUS       DASUTG010000474         5159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1407345, whole genome shotgun sequence.
ACCESSION   DASUTG010000474 DASUTG010000000
VERSION     DASUTG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407345"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF089_10810"
     CDS             complement(<1..268)
                     /locus_tag="VF089_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174498.1"
                     /translation="MENQFVDSLIQEIVQPGVDQLLNNRFFTELRQGKLSQKRIKGFA
                     LQHYHCNHAINKGLAFCMVRNANNPPLYNQFQELFTEEQSHPGLM"
     gene            complement(371..1087)
                     /locus_tag="VF089_10815"
     CDS             complement(371..1087)
                     /locus_tag="VF089_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174499.1"
                     /translation="MTACPIIEAEGLQTYYGASHILHGIDFLVRRGEAIGLMGRNGMG
                     KTTLIRTLVGHIAPRHGVIKINGHVMTQVAPYVIARQGIAYVPEGRGIFPNLSVRENL
                     IMMARPGPDGQCDWTYDRVLQTFPRLAERLSHGGQQLSGGEQQMLCIGRALMTNPTLL
                     VLDEATEGLAPLIAQEIWRIIRTIRESGIAAIIVDKNFAAVSALTDRNVILVKGRVVF
                     EGAGSELRTRPDLLQQHLGI"
     gene            complement(1084..1842)
                     /locus_tag="VF089_10820"
     CDS             complement(1084..1842)
                     /locus_tag="VF089_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174500.1"
                     /translation="MTEPILQVRQLAKTFGGLAAVDDVSLDVSVGELHAVIGPNGAGK
                     TTLINMLSGDLSPSAGSIRFGGNEVAHLSPERRSRLGIGRSYQKVNIFAAFSALENCR
                     LAAQSRDPRPWNWLRDAGSYDEIMTNARSALAQVGLSNRADSIALTLSHGEQRQLEIA
                     MSLATQPQLLLLDEPLAGLGIEEASRMVELIHRMGQTHAILLVEHDMDAVFQLARMLT
                     VMVDGRVLASGKPDEVRADEQVQCAYLGKFDEHS"
     gene            complement(1835..2842)
                     /locus_tag="VF089_10825"
     CDS             complement(1835..2842)
                     /locus_tag="VF089_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6174501.1"
                     /translation="MSPITLPRSVLLIAAAVIAIFPLVGSTFYLQLGAKIMIMAIFAL
                     SLDFLVGHTGLVSLGHAAFFGLAAYVLALISPQYQAANLWLTLPVVVVITALVALVIG
                     FLVVRTTGVYFIMVTLAFAQMLYSIFHDTNLGGGSDGIYIYVRPELAVAGIRFFNLES
                     PVQFYYFVLLIMVGVYALLSKVLHAPFGRALAGIKANEHRMRSIGYPVSSYKLGAFVL
                     GGAIAGVAGYLAACQFGFVNPEILAWHHSAGVLMMVILGGMGKLHGAVLGAFAYTLLQ
                     EILSTPFLLGSYAKHWQLAMGSLIVFIVLAFPHGLAGILDLSLRVRSTKPARLDEAAD
                     D"
     gene            complement(2847..3704)
                     /locus_tag="VF089_10830"
     CDS             complement(2847..3704)
                     /locus_tag="VF089_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6174502.1"
                     /translation="MFYLIQLLNGIQYGLLLFLLASGLTLIFGIMGVINLAHGSFYMI
                     GAYLAYWLARTTGSLWLAIPLSIIIAFTIGLLLETTIFSRLYKRDHLYQVLLTFGLIL
                     VFEELRSILFGDDVHGVPVPPLLDFSIPLTETLSYPVYRLFVTAVCISIALTMYFVIH
                     RTRLGMMIRAATSNREMAASLGVNIPWLFAAVFAFGVALAAFAGTISAPIASVSPRMG
                     NHIVIICFVVVVIGGIGSINGAMLAALAIGLADTLGKVMAPEYSAVAVYLLMAAILLW
                     RPQGIAGRA"
     gene            complement(3901..5085)
                     /locus_tag="VF089_10835"
     CDS             complement(3901..5085)
                     /locus_tag="VF089_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174503.1"
                     /translation="MDLNSSRRQFLVKAGLLTASALLPNPPVLAQSQKIRIGLMLPYT
                     GTYAQLGTAITNGFKLAVAERGGKLAGRELEYFSVDDESEPAKAPENANRLVQRDKVD
                     VLVGTVHSGVQMGMVKVAKESNTLMIIPNAGLDAATGALCAPNIFRTSFSNWQPSYPM
                     GKVLADRGMNNILTMAWKYGAGQEAVAGFNEGFIKAGGKSVRGLWIPFPNVEFQALLT
                     EIASIKPDAVFVFFAGAGAAKFIKDYNAAGLRRQIPLFGSGFLTDGVLDAVDKAAEGL
                     ETTLHYADGLNTKRDREFRAAYNKAYKSEPDVYAVQGYDAGLLLNIGLEAVKGDVTKK
                     KEMIAAMERAKIDSPRGLWTMSKAHNPIQDIYLRRVVGRENKMVSIAHKALADPARGC
                     KM"
BASE COUNT         1227 a         1428 c         1443 g         1061 t
ORIGIN      
        1 tcatcaagcc ggggtgggac tgctcttcgg taaacagctc ctgaaattgg ttgtacagcg
       61 gcgggttatt ggcatttctc accatgcaga atgccaggcc cttattgatc gcgtgattgc
      121 aatgataatg ctgtagcgca aagcctttga tgcgcttctg cgataacttg ccctgcctta
      181 attcggtgaa aaaacgatta ttgagtaact gatcgacgcc tggttgaacg atttcttgaa
      241 taagggaatc gacaaattgg ttttccatga tcaccatcct gaaggaaaaa aatcatcggc
      301 agccgaacac tctcaaagca atcataaagt gtcaagcggc tcaacggggt gcgctaacag
      361 gcgattgcag tcaaattcct agatgctgct gcagcagatc cggccgcgtg cgtaattcac
      421 tgccagcgcc ttcgaatacc acgcggcctt tgaccaggat aacgttccga tcggtgagag
      481 ccgaaacggc agcgaagttt ttgtcaacga taatggcagc gataccactt tcgcgaatag
      541 tgcggatgat gcgccaaatt tcctgtgcga tcagaggcgc gagaccttca gtggcctcat
      601 cgagcaccag caacgttgga ttggtcatca gagcgcggcc gatgcaaagc atttgttgct
      661 ctccgccgga aagctgttgt ccaccatgtg acaagcgctc ggctagccgg ggaaaggtct
      721 gcagcacgcg atcgtaggtc cagtcgcact gcccatccgg tcccggtcgc gccatcatga
      781 tcagattttc gcgaacagag agattcggaa aaatgccgcg tccctcggga acgtaagcga
      841 tgccttggcg cgcaatcacg tagggcgcca cctgagtcat gacatggccg ttgattttga
      901 tcacgccgtg acggggcgcg atgtggccca ccagggtgcg aatcagcgtg gtcttcccca
      961 tgccattgcg gcccatgagc ccaatggctt cgccgcggcg cacaagaaag tcaatgccgt
     1021 gaagtatatg gctggcgcca tagtacgtct gcagaccttc ggcctcgatg atcgggcaag
     1081 ccgtcatgag tgctcgtcga acttacccag gtacgcgcat tggacttgct catctgcgcg
     1141 aacttcatcc ggcttgccgg aagcgagcac tctgccgtcg accatgactg tgagcatccg
     1201 agccagttgg aagaccgcat ccatgtcatg ttccaccaag agaatagcgt gagtttgtcc
     1261 catgcgatgg atcagctcga ccatcctcga agcttcctcg ataccaaggc ccgccagcgg
     1321 ctcatccagc aacaatagtt ggggttgggt cgccaacgac atggcgattt cgagctgtcg
     1381 ctgctcgcca tgactgaggg tcaacgctat gctgtcagcg cgatttgaca gaccaacctg
     1441 tgcaagcgcg gatcgcgcgt tagtcatgat ctcatcataa gagcctgcgt cgcgcagcca
     1501 attccatgga cgtggatcgc gcgattgcgc tgccaagcgg cagttctcta atgcgctgaa
     1561 tgcggcgaat atgttaacct tctgatagct cctgccgatc cctaaacggg atcggcgctc
     1621 cggtgaaaga tgcgcgactt cgttgccgcc gaagcgaatg ctgccagcgc tgggcgatag
     1681 atcgcccgaa agcatgttaa tgagcgtggt tttaccagcg ccattggggc cgatgaccgc
     1741 atgcagttcg ccgacactga cgtccagcga aacatcgtcg acggctgcaa ggccgccgaa
     1801 tgttttggct aattggcgga cctgcaggat cggctcagtc atcggccgcc tcgtccagcc
     1861 gggccggttt ggttgagcgg acgcgcaacg agagatcgag aatgccggcc agcccgtgcg
     1921 ggaaggcgag cacgatgaac acgatcaagc tccccatcgc cagctgccag tgtttggcat
     1981 acgaaccaag cagaaacggc gtcgacagaa tctcttgcag caaggtataa gcaaatgcgc
     2041 cgagcaccgc gccgtggagc ttgcccatgc cgcctaaaat caccatcatt aacacgccgg
     2101 ccgaatggtg ccacgcaaga atctcgggat tgacgaaacc gaactggcac gccgcgagat
     2161 aaccggcgac tcccgcgatc gcgccgccga gcacgaaagc gccaagctta tagctcgaga
     2221 ccggatagcc gatggaacgc attcggtgtt cgttggcttt gatgcctgcc aatgcgcgcc
     2281 cgaacggcgc atgaagcacc ttactcaaca gagcataaac acccaccatg atcagaagca
     2341 cgaagtagta aaactgcacc ggactttcca gattgaaaaa tcggatgccg gcgactgcca
     2401 gttccggccg cacgtatatg taaatcccgt ccgagccgcc gcccagattg gtatcgtgaa
     2461 aaatagaata gagcatctgc gcgaaggcaa gcgtcaccat tatgaaatag acgccggtgg
     2521 ttcgcaccac aagaaaacca atcaccagcg ccaccagagc ggtgattaca accacgacag
     2581 gcaaggtcaa ccagagattg gctgcctgat attgcggtga aatcaacgcc agcacgtagg
     2641 ccgccaaacc aaaaaaagct gcgtgtccaa ggctcaccag accggtatgg ccgaccagaa
     2701 aatccaggct caaggcaaag atcgccatga tcatgatctt ggcgccgagt tgaaggtaaa
     2761 atgtcgagcc aactagcggg aaaatagcga tcaccgcggc ggcgatgagc agcaccgatc
     2821 gcgggagcgt gatggggctc acggcgtcaa gctctaccgg cgattccttg gggccgccat
     2881 aagaggattg ccgccatcag caaataaact gcgactgcgg agtattccgg cgccattact
     2941 ttgccgaggg tgtcggccag gccgatcgcc aaagccgcga gcattgcacc gttgatcgaa
     3001 ccgatgccgc cgatgaccac gaccacgaaa caaatgatga cgatgtgatt gcccattctg
     3061 ggagacaccg aggcaatcgg cgccgagatg gtcccggcaa acgctgcgag cgcgacgccg
     3121 aatgcaaaga ccgccgcaaa cagccacgga atgttgaccc cgagagaagc ggccatttcg
     3181 cgattgctgg tggcggcccg gatcatcatc ccaagtcggg tcctatggat gacgaagtac
     3241 atggtaagag caatgctgat gcaaacggcg gtgacaaaca ggcggtaaac cgggtacgag
     3301 agcgtctccg tcagtggaat cgaaaaatcc agcagcggcg gcaccggaac gccatgcacg
     3361 tcgtcgccga atagaatgct gcgcagttct tcgaagacca ggatcaagcc gaacgtgagc
     3421 aatacctggt aaagatggtc gcgcttgtaa agccggctga agatcgttgt ctcaagcagc
     3481 agaccgatcg taaaagcgat gatgatactc agcggaattg ccagccagag actgcccgtt
     3541 gtccttgcca gccaataagc cagataggcg ccgatcatat agaagctgcc gtgagcaaga
     3601 ttgatgacgc ccatgatccc aaagatcaag gtcaaaccgc tcgctagtaa aaacaacagc
     3661 agaccgtatt ggatgccgtt cagtaattgg atgagataaa acatcaacaa tgaaaactac
     3721 ggttgcagcc gttcgaaagg ttctaatggt tccagacgtt taagatgccc ttcgagctcg
     3781 atctgaaatc cgtcgataca tcgaagcatg agatagatct accattgggg tttaacgact
     3841 ggaacttttg gaacgcttgg aacattggaa ctccgccaca gccataggtt gtggcggttt
     3901 ttacatcttg catccccgcg ccggatcggc aagcgctttg tgcgcgatac tgaccatttt
     3961 gttttcgcgg cccactactc tgcgcaagta aatgtcttga atgggattat gtgctttcga
     4021 catcgtccag aggccccgcg ggctgtcgat cttggctctt tccatagccg cgatcatttc
     4081 tttctttttg gtgacgtcgc ccttcactgc ttccagtccg atgttgagga gaaggccggc
     4141 gtcatagcct tgcacggcgt agacatcagg ctcgctcttg tatgctttgt tatatgccgc
     4201 gcggaactcg cgatcgcgct tggtgttcag gccatcggca tagtggagcg tggtttccaa
     4261 gccttcggcg gctttgtcga cggcgtcgag cacgccgtcc gtcagaaagc ccgaaccgaa
     4321 gagcgggatc tgcctgcgca gcccggccgc gttataatct ttgatgaact ttgccgcgcc
     4381 ggcgccagca aaaaatacga acaccgcgtc cggtttgatc gaagcgattt cggtgagcaa
     4441 tgcttgaaat tccacattgg gaaacggaat ccaaagtcca cgaaccgatt tgccgccggc
     4501 tttgataaag ccttcattaa atccggccac ggcttcctgt cccgcgccgt atttccaagc
     4561 catagtcaaa atattgttca tgccgcggtc ggcaagcact ttgcccatcg gataggacgg
     4621 ctgccaattg gaaaatgaag tgcgaaagat atttggcgcg cacagcgctc cggtagcggc
     4681 atcgaggccg gcattgggaa tgatcatcaa ggtattgctt tctttcgcaa ctttgaccat
     4741 gcccatctga acgccgctat gaactgtgcc gaccagcacg tcgaccttgt cgcgctgcac
     4801 cagtctattg gcgttctccg gcgctttagc cggttcggat tcatcgtcga cggaaaagta
     4861 ttcgagttcg cgccccgcga gtttgccgcc gcgttccgcc accgccagtt taaaaccgtt
     4921 ggtgatggcg gtgcccagtt gggcatacgt gccggtgtaa gggagcatga gaccgatgcg
     4981 aatcttctgg ctttgtgcga ggaccggcgg attcggcagc aatgcgctcg ctgtgagcag
     5041 gccagctttg acaagaaatt gccggcggct cgagtttaga tcgatgttca tgactgcctc
     5101 cgtggagtcc taaaaggttc agcgcgggtc atggccctag tggttctcgc agtttgaac
//
LOCUS       DASUTG010000475         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_666093, whole genome shotgun sequence.
ACCESSION   DASUTG010000475 DASUTG010000000
VERSION     DASUTG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666093"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VF089_10840"
     CDS             <1..1661
                     /locus_tag="VF089_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174504.1"
                     /translation="RLPPVFFLQLLLLMLLILALAEPVFSVRVSNIAIVMDNSASMQA
                     QEDGTARVNLARDQARRVLGDLSANGKVDIFTIVPQLQKVNNEPVSPSAAIGLLAGLE
                     PYDLADAQTNHAELLNQLAADHKYERVYLITDRPLRGQSGAVRVVTVGKPRDNLAMTA
                     FTVSPSSLVSKRLEAFIEIANYSTQDQRVAVRIRADSSGALANRELRVPAGQTASASF
                     QGLPEKRFYIAEIEQSDALTLDNRRFAIPANARSLRILGISPRPQALNSLRAISGVSL
                     TIVAPEDYQKTDRSDFGLEIFHYSTPALLPLNPTLLVLPPDSNPVVKLGNPITRPVAS
                     GWRESHPLNRYLNLALFRPAYARPLIPQTAGEIIIESVQGPLAFTIEHQAKRLLVIGF
                     DPFPYLGRENLPMSIFTLNLLDWFVSAGGGRGRTTGEAIAVGAAQQQSVLTTPRGEKQ
                     VVKPGSTSFSNTFYQGIYQLNRGGEGQLLAINYQSASESDLRDSKPIELKPTENALNS
                     GSTLFSFWPYLIMASLLLFIVEWFFVPPAHPRRLPAAAPRPKLS"
     gene            1658..>3366
                     /locus_tag="VF089_10845"
     CDS             1658..>3366
                     /locus_tag="VF089_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6174505.1"
                     /translation="MTTLLADVQFAKPLLLWLLIGLPVLWFWFADRRWFVLIWRTLIL
                     AILMIVLADPQFVTQETTRESEERIFAFDVSKSISTSMRQWMDTSAQGALAPNGADRT
                     LIFAGEAKESAKWRESIKAGVAVQDPVKPENTNLQQLFESLLSLPPAPRSLYLFTDGW
                     ENHGKLELMLPAIAASGLKVYPIVPAQRMSLANVTLARLIAPTHGDAGEAVNIKVVLE
                     NHNTREVDGRLLLTRNGVTFKTDPVKLKPGSQILSYQSTLADGPLNSYRASFTSREED
                     SDQYEPDNHALAWTSVRSKGKILIVSGRTEGGRYIEEILRRQGFEVTSRTADSAPAPT
                     GFNVVIFNNVEREKLTSSYLSAIERHVNGGNGFVMLGSEWSFGPGGYRRTPIETILPV
                     ELKDPPKQEEKNRAIILVIDKSGSMREDNRILYAQEAAKAVARQLKDTDLLGVIGFDV
                     DPFVVVPLARIGSLRASFNTQIERLKPAGRTVMLPAINEAKRQLEKQDAGRKHVIILS
                     DGETGGSGGDFIDLVNVMKTEQQITVSAVAIGAEANIPLLKRITQYGGGFFHHIYDPT
                     TLP"
BASE COUNT          782 a          940 c          892 g          752 t
ORIGIN      
        1 tgcgtcttcc gcccgttttc tttcttcagt tgctcctatt gatgttactg attctggcgc
       61 ttgccgaacc ggtattctcc gtccgagtat ccaacatcgc catcgtaatg gacaactcgg
      121 ctagcatgca ggcccaggag gatggcacag ctcgtgtcaa cctcgcccgc gatcaggcca
      181 ggagagttct gggggattta agtgcaaacg gcaaagtaga tatcttcacc atcgtgccgc
      241 agcttcaaaa agttaacaac gaacctgttt cgccctctgc tgccatagga ttgctggctg
      301 gcctagaacc gtacgacctc gctgatgcac agacgaatca tgccgaactg ttaaatcagc
      361 tagccgccga tcacaagtat gagcgcgtct atctcatcac cgatcggccg ttacgaggcc
      421 aatcgggcgc cgttcgggtt gttactgtgg gaaaaccgcg agacaatctg gcgatgacgg
      481 catttaccgt ctcaccgtct tctctggtga gcaaacgttt agaggcgttc atcgaaattg
      541 ccaactattc tacccaagat caacgcgtcg cagtaaggat cagggctgat agcagtggcg
      601 ccctagcgaa tcgggaactc agggttccgg cgggacaaac cgcttccgcg tcctttcagg
      661 gtctgccgga gaaacgattc tatattgctg agattgaaca gagcgatgct ttgacgctcg
      721 ataatcggcg atttgccatt ccggcaaacg cccgtagctt gagaatcctc gggatttcgc
      781 cgcgtcctca agcgctgaac agtttgcgcg ctatctccgg cgttagcctg accatcgttg
      841 ctccggaaga ctaccagaag accgatcgtt cagatttcgg cttggagatt tttcactact
      901 caacgccggc cttactgccg ctcaatccaa cactattggt tttgcctcct gacagcaatc
      961 ccgttgtcaa gttgggcaac ccgattacac gcccggtagc gtcgggctgg cgcgaatctc
     1021 acccattgaa ccgatacctc aacttggcct tgttccgccc agcctacgcc cggccgctaa
     1081 ttccgcaaac agccggggag ataatcatcg aaagcgttca aggaccactt gccttcacga
     1141 tcgagcacca ggccaaaagg cttctggtaa tcggctttga tccttttccc tatctaggtc
     1201 gggaaaatct gccaatgtcg atcttcaccc tcaatcttct tgactggttt gtatcggctg
     1261 gtggaggtag aggtcgaacg acaggggagg caattgccgt gggggccgct caacagcaat
     1321 cggtcttaac caccccgcga ggcgagaaac aggtcgtcaa accgggctca actagctttt
     1381 ccaacacttt ctatcaggga atttaccagc tcaatcgagg cggcgaaggt cagctcctgg
     1441 cgatcaacta tcaatccgcc agtgagtcgg atctgcggga cagcaaacct atcgagttaa
     1501 aaccaaccga gaacgccctc aacagcggtt cgacactctt ctctttctgg ccctatctga
     1561 ttatggcgtc attactcctt ttcattgtcg agtggttctt tgtgccgccc gcccatccta
     1621 gacgattgcc ggcggcagcg ccccggccaa agctttcatg acgacgctgt tagccgatgt
     1681 acagttcgcc aagccattgc ttttatggct attgatcgga ctgccggtgc tttggttctg
     1741 gttcgccgat cgcagatggt tcgtcctcat ttggcgcacg ctgattttgg ccattctaat
     1801 gatcgttttg gccgatccgc agttcgtcac ccaagaaacc actcgtgaaa gcgaagagcg
     1861 catcttcgcg ttcgatgttt cgaagagcat ttccacgagt atgcgccaat ggatggacac
     1921 ctcggcgcaa ggtgcgcttg ctccaaatgg cgctgatcgc acgttaatct tcgctggcga
     1981 ggcaaaggag agcgcaaagt ggcgcgaatc gataaaagcc ggagtcgccg tacaggaccc
     2041 ggtgaagcct gagaatacaa accttcagca gctttttgaa agtttgcttt cactcccgcc
     2101 cgctccccgc agtctctact tattcaccga tgggtgggaa aaccatggca agctagagtt
     2161 gatgttgccg gccatcgctg cctccggctt aaaggtttat ccgattgtgc cggcgcagcg
     2221 gatgagcctt gccaacgtaa ccctagccag actcatagcc ccgactcatg gggatgcggg
     2281 cgaagcggtg aacatcaaag tggtgctgga gaaccacaat acccgggagg tcgatggaag
     2341 gttgcttctg actcgcaatg gcgtgacatt caaaaccgat ccggtcaagc tgaaacccgg
     2401 aagtcaaatt ttatcctatc agagcacgct tgcggacgga cccctgaatt cctatcgcgc
     2461 cagcttcacg agtcgtgagg aggactccga ccaatatgaa cccgacaacc acgcgctggc
     2521 ctggaccagc gtgcgtagta aggggaaaat cctgatcgtt agcggccgaa ctgaaggcgg
     2581 gagatacatt gaagagatcc tgcgccgcca ggggttcgaa gtgacctcac gaacagccga
     2641 ctcggcgccg gcgccaaccg gcttcaacgt agtgattttc aataatgttg aacgcgaaaa
     2701 actcaccagc agctatctca gcgcgatcga aaggcacgtc aatggtggaa atggcttcgt
     2761 gatgctgggc agcgagtgga gtttcggacc gggaggttat cgccgcacac ctatcgagac
     2821 gattttgccg gttgagctca aagatccccc caagcaggaa gagaaaaatc gagccattat
     2881 actggtcata gacaaatccg gcagcatgcg ggaagataat cggatcttgt acgctcaaga
     2941 agcggctaaa gctgtcgcgc ggcagcttaa ggacactgat ctgctcggag tcattggctt
     3001 tgacgttgat ccattcgtgg tcgtgccgct tgccaggatc ggcagcctgc gagcgtcatt
     3061 caatacacag atcgagcgcc tgaaacctgc cggccgaact gtaatgctac ccgctatcaa
     3121 cgaagctaag cgacagcttg aaaagcaaga tgccggccgg aagcacgtga tcatcctcag
     3181 cgacggtgaa accggcggca gcggcgggga tttcattgat ctggtcaatg taatgaagac
     3241 cgaacagcag attaccgtgt cggcggtcgc tatcggcgca gaggcaaaca ttccgcttct
     3301 gaaaaggatc acccaatacg gcggtggctt ttttcatcat atttacgatc cgacgactct
     3361 gccgca
//
LOCUS       DASUTG010000476         5821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1703344, whole genome shotgun sequence.
ACCESSION   DASUTG010000476 DASUTG010000000
VERSION     DASUTG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703344"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..579)
                     /locus_tag="VF089_10850"
     CDS             complement(25..579)
                     /locus_tag="VF089_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174506.1"
                     /translation="MAHSILETSLNSAATDARNTGNPMLFLNRLEPIHDNWHRRTFNA
                     RGLGFLSFHWFVIEAFKRANCPSLWSGGVRPFRPADFTNFGWSYNVTVRARVNDINSL
                     ANFSVGIESWHNDAHMAVGQAFGIEDDMMNPRVNIYYREFWRLHYFINDRFLAELRRY
                     DSSGTVTQKMNRLEQNQHTNLYRI"
     gene            complement(864..3257)
                     /locus_tag="VF089_10855"
     CDS             complement(864..3257)
                     /locus_tag="VF089_10855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6174507.1"
                     /translation="MSDIKNGALDHLRVIELGDIPASYATRWLADLGADVIKVEPPGG
                     DPSRLLPPFAGKIQDRERSLAFIHANTNKRSIVVDLEADPEREILARLLASANLLVEA
                     TPLGYLEKFGFTDECFASDFSDLVVVSITPFGRTGLYRHYKGSDAIANATGGFLFGQG
                     DNTKGQCTAPSHLAYQVASAMAAMLGLAGIRHARETGAGQRIDVSLQEALTFTNSSSI
                     ARYSRENRLERRPGGKAYGGAGTNIYRCKDGRYVHFTTNMPHMWKEFAQNWMTDKALA
                     GPEWENPRYRDQHAEEVASAFANFIGQFDADDFANQAQARHLAAAPLNTIGEFVDCEQ
                     NRVRGWLQEIDHPVIGKYKAPGAPMRLSLTPMRVRRAAPLLGQHRREILDELEQHSFN
                     SRSGSVADKRVRQPMLAGLRMADLTQQYAGPLGTELLAYYGMEVVKIESAVVPSKERE
                     AAVHACMNRGKLGCTINLRSPEGKELFKQLVKKSDVVVDNFSSGVLERLGFSYETLQQ
                     INPGIVQAVMPGWGLTGPLKSWVAWGWQLLAYTGIMRLWGYPDSPMETRCKIAWPDRV
                     GSVTMALGVLAALEYQACTGKGQFIEAGMLEAQGSMMGPAILDYTINGNEWDALGYRE
                     ILGEPYAPYGCYPCYGQDNWIIIACAGDEQWRSMVHLIGDSSWAADPKFAAQAGRKQD
                     RDELDRKLSEWTRKYTAKQLFRVLQETGVAAGIPSSGEDLFHDIHLRARGHIVETEAR
                     PWGKITHHGLPGIPSVSAASAARPAPWMGVNNDQVFGEILSLSNEKVAELKEKGAIK"
     gene            complement(3556..4566)
                     /locus_tag="VF089_10860"
     CDS             complement(3556..4566)
                     /locus_tag="VF089_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HEX6174508.1"
                     /translation="MSMPLPQTFKAMVVSETGDKKFPREIKQVALSDLPAGELVIEVK
                     YSSLNYKDALSATGNKGVTRKYPHTPGIDAAGVVVNSSSRDIAIGSEVIVMGYDLGMN
                     TAGGFGQYISVPAGWAVKLPAGLTLRDSMAHGTAGFTAALCVMRLLAGGLNKEAGEVL
                     VTGATGGVGSVAVGILAKLGFDVVAATGKTAEKDFLTRLGAKAIISREEANDTSGRPL
                     QKGRWAGAVDTVGGNILATALKSAKYGGLVTACGNAMSAELSVSVFPFILRGVSLLGV
                     DSVEIPMTTRTRVWQKLATEWKLDMSGDLITECSLEELNPKIDEILKGGIRGRVLVNL
                     SK"
     gene            4790..>5821
                     /gene="prpC"
                     /locus_tag="VF089_10865"
     CDS             4790..>5821
                     /gene="prpC"
                     /locus_tag="VF089_10865"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX6174509.1"
                     /translation="MATDKTGGLAGIIAGNTSIATVGKEGKGLTYRGYSIYDLAEHST
                     FEEVAYLLIYGKLPSASQLREYKENLRKQRGLPSPLKTVLEQIPGSTHPMDVLRTACS
                     MLGTLEPENKEHDQIAIANRLTACFSSMLLYWHHYVNSGKRIDTKTDDDSSAGHFLHL
                     LHGKKPDELCRRAVDVSLILYAEHEFNASTFTARTIASTLPDFYSAVTGAIGALRGPL
                     HGGANEAAMELLEKFSTPEEAESGILEMIGAKKLIMGFGHRVYKISDPRSDVIKAWAK
                     KLADAQGDKKLYAVSERVEQVMRREKNMFPNLDFYSASAYHFCGIPTAMFTPLFVVSR
                     ITGWAAHIIE"
BASE COUNT         1301 a         1686 c         1549 g         1285 t
ORIGIN      
        1 ttgtcaacgc taaatgcggt aaagctaaat acggtataga ttggtatgct gattctgctc
       61 aagacgattc atcttctgag tgacggttcc gctggagtcg tagcgacgca gctcggcgag
      121 gaacctgtcg ttgataaagt agtggagacg ccaaaattct ctgtagtaga tatttactcg
      181 cggattcatc atgtcatcct cgatgccaaa cgcttgtccg accgccatat gcgcgtcatt
      241 atgccatgac tcgatgccca ctgagaagtt agccaatgag tttatatcgt ttacacgcgc
      301 acgtaccgtc acgttatagc tccagccgaa atttgtaaaa tctgcgggac ggaatgggcg
      361 aacgcctccg gaccaaagag atgggcagtt cgctcgcttg aacgcttcaa ttacgaacca
      421 atgaaaactc aggaacccaa gcccgcgagc attgaaggtt cgtcggtgcc agttgtcatg
      481 gattggttct aaacgattga ggaacaacat tggattaccc gtattgcggg cgtccgttgc
      541 cgcagaatta agagaagtct cgagaatcga gtgcgccatt cgaacctcct gcattttatt
      601 tggcgagctg acgtactgga acttcttagc tattcatcaa atattcatcg aaaaaaagac
      661 acagcgacgt taggctttcg ttatagactt aatcgctgaa cgcgatccag gtgccgatgt
      721 gttttctgag cttaatgaac cgcaaggagc aatgaatcta aacgcgtaca gccaagagcc
      781 tgctccggtc gatgtcgacc atgggctcaa tggtctggga gattcgaaaa cttgcgatta
      841 tttgcgcgag ccttttacgg caattacttg atggcgccct tttctttaag ctctgctact
      901 ttctcgttac tcaaactcag aatctcacca aacacttgat cattattcac gcccatccag
      961 ggcgcaggac gcgcggcact ggcagccgaa acagaaggaa ttcccggcag cccatggtgt
     1021 gtgattttgc cccagggtcg cgcttcggtc tcgacgatat ggccgcgcgc gcgcaagtga
     1081 atgtcatgaa atagatcttc gcccgaagat ggaattcccg ccgccacgcc ggtctcttgc
     1141 agcacccgaa ataactgttt tgccgtgtac ttacgtgtcc attccgaaag tttccgatca
     1201 agctcatcac gatcttgttt acggccggcc tgagccgcga acttcggatc agcagcccag
     1261 gaggaatcgc cgatcaaatg caccatactg cgccactgct cgtcgccggc gcaggcgatg
     1321 atgatccaat tatcctggcc ataacaggga tagcagccgt aaggggcgta cggttcgccg
     1381 agaatttccc gatagcctag cgcatcccat tcgttgccgt tgatcgtgta gtcgagaatc
     1441 gccgggccca tcatcgagcc ctgagcctcg agcatgccgg cttcgatgaa ttggcctttg
     1501 ccggtgcacg cttgatattc cagcgccgcg agcacaccca gcgccatagt gaccgacccc
     1561 acccgatcgg gccaggcaat cttgcagcgg gtttccatcg gtgaatcggg gtatccccaa
     1621 agccgcatga tcccggtata ggccaggagc tgccagcccc aggcgaccca cgattttagc
     1681 ggcccggtca atccccaacc cggcatgacc gcttgaacga ttcccggatt gatctgctgc
     1741 aacgtctcgt agctgaagcc caggcgctcc agaacgccgg agctaaaatt atcgacgact
     1801 acatcggact tcttgaccaa ttgcttgaac aattccttgc cctcagggct gcgcagattg
     1861 atcgtgcaac cgagcttgcc gcgattcata caagcgtgca cggcggcttc gcgttctttg
     1921 ctaggcacga ccgcggactc gattttcacc acctccatgc cgtagtaggc cagcagttca
     1981 gtaccgagcg gaccggcata ttgctgcgtt aggtcagcca tacgcagccc ggcgagcatg
     2041 ggctggcgaa ctcgcttgtc agcgactgaa cccgatcgtg agttgaaaga gtgttgttcc
     2101 agctcgtcaa gaatttctct tcggtgctga cccagcaatg gcgccgctcg ccggacgcgc
     2161 atcggcgtca gcgatagacg catgggcgcg ccgggcgcct tgtacttgcc gattacggga
     2221 tgatcgatct cctgcagcca tccgcgcacg cggttttgtt cacaatcgac aaactcgccg
     2281 atcgtattga gcggcgcggc agcgaggtgg cgcgcctgcg cctggttggc aaaatcatct
     2341 gcatcgaact gaccgatgaa attggcaaac gcgctggcga cttcctcggc atgctgatcg
     2401 cggtaacgcg ggttctccca ctccggcccg gccaacgcct tatcggtcat ccaattttgc
     2461 gcgaattcct tccacatatg cggcatgttg gtggtgaaat gaacgtagcg gccgtccttg
     2521 cagcggtaga tgttcgtccc agcgccgcca taagctttgc cgccgggccg gcgttccaat
     2581 cgattttccc ggctgtaacg ggcaatcgaa ctgctgttgg tgaacgtcaa ggcttcttgc
     2641 agcgaaacat cgatgcgctg accggcaccc gtttctctgg cgtgacggat tccggccaat
     2701 cccaacatcg ccgccatcgc gctggctact tggtaggcga gatggcttgg cgcggtgcac
     2761 tgccctttgg tgttgtcgcc ctgaccgaaa agaaatccac cggtggcatt ggcgatggcg
     2821 tcactgcctt tgtaatgccg atagagtcca gtcctgccaa atggtgtgat cgaaacgaca
     2881 accagatcgg agaaatcgct ggcgaagcac tcatcggtaa agccaaactt ctccaggtaa
     2941 ccaagcggcg tcgcttcgac caggagattc gcggatgcga gcaaccgggc gaggatctca
     3001 cgctcgggat cggcttccaa atcgacaacg atgctgcgct tgtttgtatt cgcatgaatg
     3061 aacgcgaggc tgcgctcgcg atcctgaatc ttgccggcaa acggcggtag caggcggctg
     3121 ggatcgccgc cgggtggttc gactttaatc acatcggcgc cgagatccgc cagccaacgt
     3181 gtcgcataag acgcgggaat gtcaccgagc tcgatgacgc gcaggtgatc taaagcgccg
     3241 tttttgatat cagacaatgc gtctcctccc tcagtcaatc aagttctctc gtttttccgt
     3301 tccaggataa ttcccgcagc ctgttgcgta gaattttccc ctccgaagtc cgtcattccc
     3361 gcatgctact agcgggaatc caggcgaaac gatagcttgg acccccggta aaaacattcg
     3421 ggggtgacga gattcagata acgatcatca agctctggtg ataccccgct gtttgcggcg
     3481 gattcttcat tctcacctct gccttgcttc cgggagagcc aacgcagacg agatttctac
     3541 gaaaaagctg ttgtattact tgctgaggtt gaccagcact ctgccgcgaa ttccaccttt
     3601 gagaatttcg tcgatcttag gattcaactc ttccaaggaa cattcagtta taagatcgcc
     3661 tgacatatcg agtttccact cggtggcaag cttctgccac acgcgcgtgc gggtggtcat
     3721 cggaatctcg accgaatcca ccccaagcaa actgacgccg cgcaagataa acggaaacac
     3781 gctgactgat agttcagcgg acatggcatt gccgcacgct gtgacaaggc cgccgtactt
     3841 cgctgacttg agcgcggtgg ccaggatgtt gccgccgaca gtatcgaccg cgccggccca
     3901 gcggcctttc tgaagcggcc ttcctgaagt gtcattagcc tcttcccgag agattatggc
     3961 tttggcgccg agccgggtga ggaaatcctt ttcggcggtc ttgccggtcg cggcaacgac
     4021 gtcgaagcct aacttggcga gaatgcctac ggccacgctg ccgactccgc cggtggcgcc
     4081 ggtcaccagc acttcgccgg cttccttgtt gagacctccg gccagtaatc tcatcacgca
     4141 caacgccgca gtaaatccgg cggtgccgtg ggccatgctg tctctcaagg ttagcccggc
     4201 cggcaacttc accgcccaac ctgcgggcac gctaatgtac tggccaaaac ctccggcggt
     4261 attcatccca agatcgtaac ccatgacgat gacctcgctc ccaatggcga tgtctctgct
     4321 gctggagttg acgaccacac cggcagcatc gattcccggc gtatgcggat atttgcgagt
     4381 cactccttta ttgccggtgg ccgacagcgc atccttgtag ttcaacgagg agtatttaac
     4441 ctcgatgacc aattcacccg caggcaaatc gctcagggcc acctgcttta tctctcgggg
     4501 aaattttttg tcgccggtct cggaaacaac catcgcttta aaagtctgtg gtaaaggcat
     4561 gctcattact cctcgtccgt aataaaaata gtcacagcaa gcgagtacat aacctgcagc
     4621 gcgaactgtc aaggggcggc ctgaaacgga tctgcgcccg cctgccaaac cacgcgaaga
     4681 tcatcttcgc agcgaaagac tttgagcgcg gagattgacg gaagaagtcc gacaacttgc
     4741 ttgcaatagg tattcggttc agggcaaagt ggagcctgga ggaattctca tggcaaccga
     4801 caaaacgggt ggcttagctg gaatcattgc cggtaacaca tcgattgcga ccgtcggcaa
     4861 agaaggaaag ggactgacct atcgcggcta ctccatctat gacctggccg agcattcgac
     4921 cttcgaagaa gtcgcctatc tgctgatcta cggcaaattg cctagcgcat cacagctccg
     4981 cgaatacaag gaaaatttgc gcaagcagcg cggattgccg tcgccgctca aaaccgtgct
     5041 cgagcagata ccggggagca cccacccgat ggatgtcctg cgcactgcct gttccatgct
     5101 cggcacgctg gagccggaga ataaagagca tgatcagatc gccatcgcca accggctgac
     5161 cgcatgcttc agctcgatgc ttttgtattg gcatcattac gtcaatagcg gcaaacgcat
     5221 tgataccaag accgacgacg acagcagcgc cggccatttt ctgcatctcc tgcatggcaa
     5281 aaagccggac gaactctgcc gtcgcgcggt tgacgtctcg ctgatcctgt atgccgagca
     5341 cgaatttaat gcttcgacct ttactgcgcg caccatcgcc tccaccttgc cggactttta
     5401 ttcggcggta accggcgcca tcggcgcttt gcgcggcccc ctgcatggcg gcgccaatga
     5461 ggctgcgatg gagttgctgg aaaagttttc tactcccgaa gaagctgaga gcggaattct
     5521 cgaaatgatc ggcgccaaaa agctgatcat gggttttggt catcgcgtat ataaaatctc
     5581 cgatccgcgc tccgacgtga tcaaagcctg ggccaaaaag ctcgccgacg ctcagggaga
     5641 caaaaagctc tacgcagtat ccgaacgggt cgagcaggtc atgcgacgag aaaaaaacat
     5701 gtttcccaac ttggatttct acagcgccag cgcctatcac ttctgcggca tcccgacggc
     5761 gatgttcacc ccgttgttcg tggtctcgcg catcaccggt tgggccgcgc acatcatcga
     5821 a
//
LOCUS       DASUTG010000477         6933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1703674, whole genome shotgun sequence.
ACCESSION   DASUTG010000477 DASUTG010000000
VERSION     DASUTG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703674"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VF089_10870"
     CDS             <1..1072
                     /locus_tag="VF089_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX6174510.1"
                     /translation="IVTRGIPLTQVGRLLALILPTFLELTLPMAFLLAILLGLGRLSG
                     DQELLGLKSSGVSPVQILLPLTFLAASVALITLLLTTWVRPTAQVALKNELYDIAKTR
                     IGTALREKVFNDDFPNILIYVEEIIPPGNVAQGILIIDKRETAKDNIILGKVGLINTE
                     EQTHTLSLRLFDGSIYERDRTRSGFSQTNFHIYDFKLDLDEFIAPAKMKDAGPKEMSL
                     SRLLKTIEDKQAAGIIARPELMEFHQRIAFSFAPIVFCLLGVSLSLLPRTSRANRSWG
                     VALCIVWLVIYYALLSLGKAFGERGALHPFVAMWLPNIIVGVISIHLFRKALYESPLL
                     LQTKFDAASLWLGQKLTRFENR"
     gene            1082..2194
                     /gene="lptG"
                     /locus_tag="VF089_10875"
     CDS             1082..2194
                     /gene="lptG"
                     /locus_tag="VF089_10875"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX6174511.1"
                     /translation="MQQKPRFAHLPFGSIVDRYIASGFVRVFVASLLCFTAFFAIIEF
                     FDRIGTLVESGISITTALRYVLYKIPLSISRVIGFATLFSTLFCLGMLYKAQEITAMQ
                     AGGLSTRRLSFPLLCLSIFICVTNFIWNEGLVPVFAHRAETIYKTEIKKKQQQSLFGT
                     KDIWIRGEGGFVNIDSFDPDNNVLEGITFFVLSRDFTLQSTIGVPKAQWVGQRWDSSS
                     GIEWQFLSDGKLRQRIVNTPPTISETPDDLVLLARDPEEFTFFDLQKQIAEMKTKGMD
                     ATAYEVDLQVKLAMPFISPLMVLIAIPLALKRRIGSGLALSFGTAMLIAFGYWVLSAF
                     CFSLGHSGALPPILAAWLPNSVSALLGIYLFTAEEY"
     gene            complement(2316..2708)
                     /gene="rpsI"
                     /locus_tag="VF089_10880"
     CDS             complement(2316..2708)
                     /gene="rpsI"
                     /locus_tag="VF089_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6174512.1"
                     /translation="MAEKVITATGRRKTSVARVFLRLGKGAIAINNRTLNEYFGRETA
                     RMIVMQPFELTQTAGSYDIDVNVNGGGSSGQAGAIRHGITRALMLLNPDLRSSLKKAG
                     FVTRDPRGVERKKYGRHKARKRPQYSKR"
     gene            complement(2722..3147)
                     /gene="rplM"
                     /locus_tag="VF089_10885"
     CDS             complement(2722..3147)
                     /gene="rplM"
                     /locus_tag="VF089_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX6174513.1"
                     /translation="MNTKEALAQREWYIVDADGKILGRMASEIARVLRGKNKVIFTPN
                     ADTGDFVIVINAKRVRLSGHKLDDKIYYRHTNYPGGIRSRTAGNLLENKPEDLIHLAV
                     KGMLPKNRLGRKLAAKLKVYAGAEHPHEAQKPKPLAIQA"
     gene            complement(3189..3265)
                     /locus_tag="VF089_10890"
     tRNA            complement(3189..3265)
                     /locus_tag="VF089_10890"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3229..3231),aa:Asp,seq:gtc)
     gene            complement(3283..3357)
                     /locus_tag="VF089_10895"
     tRNA            complement(3283..3357)
                     /locus_tag="VF089_10895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3323..3325),aa:Val,seq:tac)
     gene            complement(3413..3685)
                     /locus_tag="VF089_10900"
     CDS             complement(3413..3685)
                     /locus_tag="VF089_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003659331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6174514.1"
                     /translation="MTKADLIDSLARRAEMPRQKAEEIVNGFLDDIVAALKNGDKINV
                     SGFGTFSISERKGRTGRNPKTGEAIEIAPSRVAKFKAGKLLKEALS"
     gene            complement(3791..6259)
                     /gene="lon"
                     /locus_tag="VF089_10905"
     CDS             complement(3791..6259)
                     /gene="lon"
                     /locus_tag="VF089_10905"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX6174515.1"
                     /translation="MLFRSDKKDEKEKDSGGSTRVPLLPLRDIIVFPHMVVPLFVGRQ
                     RSIRALEEATQKQGPIFLSSQKDAKTNEPAEDDIYKIGTLGTVVQMLKLPDGTVKVLI
                     EGKRRAQIARFVNNPEFFLVDVEEAPEVNDRNTEVEALTREVHTTFENYVKLKKKIPP
                     EMVMSVSSIDDPGRLADTIVAHLGIKLEDRQNLLETFNAAERLEKVLGHMRAEIEILE
                     VERRIRSRVKKQMERSQKEYYLNEQMRAIQKELGEKDEFKNEIQEIEEKIKQKKLSSE
                     AREKIDKELKKLKMMSPMSAEATVVRNYIDWILSLPWNEFTDDKLDITEAERVLEEDH
                     YGLEKVKERILEYLAVQSLVGKIKGPILCLVGPPGVGKTSLGRSIARATGRKFVRVSL
                     GGVRDEAEIRGHRRTYIGALPGKIVQSMKKAGSSNPVFLMDEIDKMSTDFRGDPSSAL
                     LEVLDPEQNTAFNDHYLDLDYDLSKVMFITTANTLDRIPRPLQDRMEIIRIAGYTELE
                     KLSIAKRYLLEKQKEANGLTPENVTFTDNALLGVIRHYTKEAGVRSLEREIAAICRKV
                     AVEVVKKDRNTHMQVGTKSLHKYLGPVKYRYGKAEDEQKVGVTTGLAWTELGGELLAT
                     EVTVMPGKGQLIITGKLGDVMQESAQAAMSYVRSRATELGLDKDFYQKIDVHIHVPEG
                     AIPKDGPSAGITMATSLVSALTTVAVRNDLAMTGEITLRGTILPIGGLKEKVLAAHRA
                     GIKKVLIPAENEKDIEEIPAAVLKTVEIELVAHMDDVLKKALAIDNPENLFKRPPASL
                     ESKERPNPFGEKIEDNAPAEILPQ"
     gene            complement(6273..>6933)
                     /locus_tag="VF089_10910"
     CDS             complement(6273..>6933)
                     /locus_tag="VF089_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174516.1"
                     /translation="RDVSGEGVQQALLKIIEGTMASVPPKGGRKHPQQEFLQVDTTNI
                     LFICGGAFVGLEDIIRKRTGKKRMGFGGHLDEQDGKRPTELLHETRPEDLLKFGLIPE
                     FIGRLPVIATLDELDEFALVRILKEPKNALTKQYSKLFEMESVHLKFTEEALRSIARD
                     AMKRKSGARGLRAIMENIMLDIMYDMPSQPNIKEVVISEEVVNKNEPPIVVYTKAAES
                     A"
BASE COUNT         1558 a         1856 c         1595 g         1924 t
ORIGIN      
        1 tatcgtaact cgtggcatac ccctgaccca agtcggaaga ctacttgctc tgattctgcc
       61 aacgttctta gaattaacgc ttccaatggc gtttcttttg gctattctct tggggctcgg
      121 cagactatca ggcgatcagg aacttctcgg ccttaaatcg tccggagtca gtccggtgca
      181 gattctgtta cccctcactt ttctggcggc gagtgtcgcg cttataacct tgctcctgac
      241 gacctgggtt agaccgacgg cgcaagtggc tcttaaaaac gaactttacg atatcgctaa
      301 gactcgcatt ggcactgccc taagggagaa ggtattcaac gatgactttc caaatatatt
      361 aatctatgtc gaagagataa tacctcctgg gaatgttgca caaggcattc taatcataga
      421 caaacgcgag accgcgaagg acaatattat tctcggaaag gtggggctaa tcaacaccga
      481 ggaacagaca catacgctgt cgttgagatt gtttgatggc tccatttatg aacgagatag
      541 aacacgctca ggtttcagcc aaactaactt ccacatctat gatttcaagc tggatttgga
      601 cgagttcatc gcacccgcaa agatgaaaga tgccggacca aaggaaatgt ctctaagtcg
      661 cctactcaaa accatagagg acaagcaagc tgccggcata atcgccagac ctgaactcat
      721 ggagtttcac caacgtattg cgttctcctt tgcgcctatc gtgttctgcc ttctcggtgt
      781 ttctctgtcg cttctaccgc gcacatcacg ggccaataga tcctggggag tggctttgtg
      841 catcgtatgg ctcgtgatct actacgctct cctttcgctt ggaaaggcat ttggagagag
      901 gggtgctctt cacccgttcg ttgccatgtg gcttccaaac atcatcgtcg gtgtcatttc
      961 gatccatcta ttccggaaag cgctttacga atcgccatta ctgttgcaga ccaagttcga
     1021 tgcggcatct ctgtggctcg ggcagaaact caccagattc gaaaaccgct aatcgaagat
     1081 catgcaacag aagccgagat ttgcccattt accgtttggc agcatcgtcg atcgctacat
     1141 cgccagcggc tttgtcagag ttttcgtcgc cagcttgctt tgttttactg cgttttttgc
     1201 gatcatcgag ttttttgacc ggatcggcac cttggtggaa tctggaattt cgattactac
     1261 ggcactacgc tacgtgttgt acaaaattcc gttatcgata tctcgggtga tcggctttgc
     1321 cactctcttt tctactctct tttgcctggg aatgctttat aaagctcagg agatcacagc
     1381 aatgcaggct ggcggcctga gcacgcgaag attgtcattc cctctacttt gtctctcaat
     1441 atttatttgc gtgaccaatt tcatctggaa tgaaggacta gtaccggttt ttgctcatcg
     1501 cgccgagacg atctataaga ctgagatcaa aaagaaacag caacaaagtt tattcggaac
     1561 aaaagatatc tggatccggg gggaaggagg ctttgttaat atcgatagct ttgatccaga
     1621 caacaatgtt ttggagggaa tcacgttctt tgttttaagc cgcgacttta cattgcagtc
     1681 tacgattggc gttcccaaag cacaatgggt gggacagcga tgggactcaa gcagtggtat
     1741 cgaatggcag tttttatcgg acggaaagct aaggcaacgg atcgtgaaca cgccgccaac
     1801 gatctccgaa acacccgacg acctcgtgtt acttgctcgc gatcccgaag aattcacgtt
     1861 tttcgatctg caaaaacaaa tagctgaaat gaagaccaaa ggcatggatg ccacagctta
     1921 tgaggtcgat ttgcaagtaa agctggccat gccatttatt tcgcctctga tggtcctaat
     1981 tgctattcct cttgccttga agaggcgcat tggcagtggc ctagcattaa gttttggcac
     2041 cgccatgctg atcgccttcg ggtattgggt gctttcagcc ttctgctttt ccctgggaca
     2101 tagcggcgcc ttgcctccca tcctcgcagc atggttgcca aattcggtct ccgcgctgtt
     2161 gggaatttat ctttttacag cagaagagta ctagtgaaga aaatatggcc gtgattgctg
     2221 gccgacgatg cggcagaatc taaacgataa ctaagagccg cccaacttgc gccgactgaa
     2281 tcgcgcaata gatctcgaac gtagtttcgc ctcacctaac gctttgagta ctggggtcgt
     2341 tttcgcgcct tgtgtcgacc gtattttttg cgctcaaccc cgcgcggatc acgcgtgacg
     2401 aaaccggcct ttttcaagga cgatcgaaga tccggattga gcaacatgag cgctcgcgtg
     2461 atgccatgac gaatggcacc tgcttgccct gaactacccc cgccgttaac atttacgtcg
     2521 atgtcataac tccccgcggt ctgggttaat tcgaaaggct gcatgacaat catgcgagcg
     2581 gtctcccgac cgaaatactc attgagagtt cggttattaa tcgcgatggc gccctttcca
     2641 agacgtaaga agaccctagc tacagaagtc tttcttctcc cggttgcggt aatcactttt
     2701 tctgccattt gatactctct cctaggcctg gatcgccagc ggttttggct tttgtgcttc
     2761 gtggggatgc tccgcgcctg catacacttt aagcttcgcg gctagtttcc tacctaaccg
     2821 gttcttgggc aacattcctt taacagctaa gtgaatcagg tcttccggtt tgttctcgag
     2881 aaggtttccg gccgtgcgcg atcgaattcc acctggataa ttagtgtgac gataatagat
     2941 tttgtcatcg agtttgtgtc ccgaaagcct cacacgtttc gcattaataa caatgacaaa
     3001 gtcgccggta tccgcattgg gagtgaaaat caccttgttc ttaccgcgca gaacccttgc
     3061 gatctcgcta gccatgcggc ctaagatctt tccatccgca tcgacgatgt accattctcg
     3121 ttgtgcaagc gcttctttgg tattcattac tgctgtcccc acgatatgtc tgtgccactc
     3181 ggacgaaatg gcggggccga cgagactcga actcgcgacc tccggcgtga caggccggcg
     3241 ttctaaccaa ctgaactacg accccatgtg attaactacg attggtgggc ggtacaggat
     3301 ttgaacctgt ggcccccggc ttgtaaggcc gatgctctcc cgctgagcta accgccctct
     3361 ccggaacttt atctggtgtg tacgaccagc ttcgttttgg cacggcagcg cgttagctga
     3421 gggcctcttt taggagctta ccggccttga acttagcgac tctagaagga gcgatttcga
     3481 ttgcctcccc agtcttcgga tttctcccgg tccgcccttt acgttccgag atagaaaaag
     3541 tcccgaatcc ggaaacattg attttatccc cgttctttaa agcggcaaca atgtcatcaa
     3601 gaaatccatt gacaatttct tctgcttttt ggcggggcat ttcagcacgt ctcgctaatg
     3661 aatcgattaa gtctgccttg gtcattactc ttccattcaa tcaaatctgt ctaagtcaaa
     3721 cgttgcgggt ttataacagt cggccagccg cgtgtcaaat cacaaggctg gccgactatc
     3781 tgaacagcag ttattgagga agaatctcag cgggagcgtt atcctcgatc ttttcgccaa
     3841 acggatttgg cctctctttc gattctagtg atgccggcgg gcgcttgaaa agattctctg
     3901 gattgtcaat tgccaacgcc ttcttcaaca cgtcatccat atgagcgacc aattcaatct
     3961 ccactgtctt caaaaccgca gcaggaatct cctctatgtc tttctcattc tcggcaggta
     4021 ttaacacctt cttaattcct gcgcgatgcg cagccaatac cttttccttg agccctccaa
     4081 tcggaaggat ggtgccacgc agtgtgattt cccccgtcat agcaagatca tttcgcactg
     4141 caaccgtcgt caatgccgaa actaatgatg tcgccatggt tattccagcc gaaggtccat
     4201 ctttagggat agctccttcc ggaacgtgga tgtgaacatc aattttttga tagaagtctt
     4261 tgtccaaccc aagctccgtt gcccgagacc gcacgtaact catggcagcc tgcgcggact
     4321 cttgcatgac atcgcccagt tttccggtaa tgatcagctg gcctttccct ggcataacgg
     4381 tcacttcagt cgcaagcaat tcaccgccga gttccgtcca cgccagaccg gtcgttactc
     4441 caaccttttg ctcatcttca gccttgccgt agcggtactt cactggtcct aaatatttgt
     4501 ggagactctt ggttcccacc tgcatatgag tgttacgatc tttcttgacc acctcaacgg
     4561 caacctttcg gcagattgct gcgatctctc gttcgaggct tcttacaccg gcttctttgg
     4621 tatagtgccg aatgacccca agcagtgcat tgtcggtaaa tgtcacgttt tcgggagtga
     4681 gcccattggc ttccttctgc ttctccagaa ggtaccgttt agcaatgctg agcttctcca
     4741 gttcggtata gccagcgatt cgaataatct ccattcggtc ctgcagtggc cgcggaatac
     4801 gatcaagagt atttgctgtc gtgataaaca tcactttgga caagtcataa tccaagtcca
     4861 ggtagtgatc gttgaacgcc gtattctgtt caggatctaa gacctcgagc aatgcagagg
     4921 aaggatcgcc tcgaaaatca gtcgacattt tgtcaatctc gtccatcaaa aatacaggat
     4981 tgctcgaccc cgcttttttc atcgactgga cgatttttcc gggcaaggcg ccaatgtacg
     5041 tgcggcgatg cccgcggatt tccgcctcgt cccgtacgcc gcctaaagac actcgaacaa
     5101 attttctccc ggttgcgcgt gcgatcgatc gccccaatga agtttttccg acccccggag
     5161 gcccgacgag gcacaggatt ggacctttga ttttacccac gaggctttgc accgcgagat
     5221 attcgaggat cctctctttt accttctcaa ggccgtagtg atcttcttct agaacccgtt
     5281 cggcctcagt gatatcaagc ttgtcatcgg tgaattcgtt ccagggcaag cttaggatcc
     5341 agtcgatgta gttcctgact actgtagctt ccgctgacat cggcgacatc atctttagct
     5401 tcttgagctc cttgtcgatc ttttcgcgag cttctgacga tagcttcttc tgcttaattt
     5461 tctcctcgat ttcctggatc tcgtttttga actcgtcttt ttcgccaagt tctttttgga
     5521 tcgcgcgcat ttgctcgttc agatagtatt ctttttgcga acgctccatc tgtttcttga
     5581 cacgggaacg aatacggcgc tcgacttcga gtatttcaat ttccgcacgc atatgcccta
     5641 agaccttttc cagccgttcg gccgcgttga aggtttcgag cagattttgc cgatcttcta
     5701 acttgattcc caagtgagcc acgattgtgt cagctagccg tcccggatca tcgatggagg
     5761 acaccgacat caccatctca ggagggattt tcttcttcag cttgacgtaa ttctcaaacg
     5821 tggtatgaac ctcgcgagtt aaagcttcta cctcagtatt acggtcatta acctccggag
     5881 cttcctctac gtctaccaag aaaaactcag gattgtttac aaaccgcgcg atctgcgcgc
     5941 gccttttccc ttcgatcaaa acctttacgg tgccgtccgg aagtttgagc atttggacga
     6001 ccgtccccaa tgtgccgatt ttataaatat cgtcctctgc cggctcgttc gtcttcgcat
     6061 ccttctgtga cgacaagaag atcggtcctt gtttttgggt agcttcttcc aaggctcgaa
     6121 tagaacgttg acggccgacg aaaagcggca cgaccatatg gggaaagaca attatgtccc
     6181 tcaaaggtaa caacgggaca cgggtggagc cacccgaatc tttttccttc tcatcctttt
     6241 tatcactgcg aaacaacata ccgtttccct tcctaagcgc tctccgccgc tttggtgtac
     6301 acaacaatag gaggttcgtt tttgttgacc acttcctcgg aaatcacgac ctccttgatg
     6361 ttgggctgcg agggcatgtc atacataata tcgagcatga tattctccat gattgctctt
     6421 agccctcgcg cgccggattt acgtttcatc gcgtcacgcg caatcgatcg aagcgcctct
     6481 tccgtgaact taaggtgcac gctttccatc tcgaaaagct tggagtactg tttagttagc
     6541 gcattcttcg gttcttttag tatacgcact agagcgaatt catccagttc gtctaacgta
     6601 gcaatcaccg gcaatctccc gataaactcc gggatcaaac cgaacttaag cagatcctcg
     6661 ggccgcgttt catgcaaaag ctcggtcggc cgtttgccat cttgctcatc gagatgaccg
     6721 ccgaagccca tccgtttttt cccggtccgc ttgcggataa tatcttcaag accgacgaaa
     6781 gctccgccac aaataaataa aatattggtc gtgtcgacct gtaaaaactc ctgctgcggg
     6841 tgctttctac cgcctttggg tggaacgctc gccatggttc cttcgataat ctttagcagg
     6901 gcctgttgca cgccttcacc agagacatcg cgg
//
LOCUS       DASUTG010000478         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1703859, whole genome shotgun sequence.
ACCESSION   DASUTG010000478 DASUTG010000000
VERSION     DASUTG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703859"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF089_10915"
     CDS             <1..216
                     /locus_tag="VF089_10915"
                     /inference="COORDINATES: protein motif:HMM:NF025097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX6174517.1"
                     /translation="VRGVNVVGLKRAGFDDRRIRAIRNAFRVLFRKGRNLSIALTEVE
                     ESMRADDDVMTLVEFIRSSKRGVCFGG"
     gene            complement(213..614)
                     /locus_tag="VF089_10920"
     CDS             complement(213..614)
                     /locus_tag="VF089_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174518.1"
                     /translation="MKKHISKGIALASFVTVMSAPIHAQTTAPVDQPPEQPVSQELGY
                     GFASALASVFYIPAKVTYAGLGLVTGGLGYVLTGGRTDVASNIIYPAVRGHYVVTPNH
                     IKGIEPIYFVGAPPESPEPESYAAGTPTPTR"
     gene            complement(774..>3223)
                     /gene="glnE"
                     /locus_tag="VF089_10925"
     CDS             complement(774..>3223)
                     /gene="glnE"
                     /locus_tag="VF089_10925"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX6174519.1"
                     /translation="FENDEGESSGGTKGKAEPRAFFADIGKKIIQAMGKVTEDGFVFR
                     IDLRLRPLGVNGPLVQSVDSAMLYYESWGQCWERAALIKARAVAGDKKLGVRFLKEVE
                     PFVYRRYLDYTTVEELRHMKTRIENELLNSQDKERNIKLGFGGIREIEFFAQALQLVN
                     GGYEPALRQQNTLRALSELAAHKFISAEEMNQLAEAYRFLRQVEHKVQMVQEAHAHSI
                     PEGEEEEALARRLGYLPKGERSEREQFWSEKNIHSENVRRIFDRLFYGAQKEITHEGQ
                     SHAGTIWHDLDQRELIIRELEKLGFADASKAYENLLAVRDGESFAPPSAKRLKIMRTL
                     GPALIAEIAKSGAPDQALLNLSRFSHRIGGRSGFLRLLAENPETMRLLITLFADSQFL
                     TELFLTRPELIDTLIRVDLTRIDKSKESMMAGLREAIAEQDDVEDKLNAIRRSKTEEF
                     IRIGLHDLGGTIDIEPVLEQLSDLADAVVECALQLTLEELQQNFGIISQGRFAVLGMG
                     KMGGRELDYNSDLDLVFVYDADDDAHSSGGVRGKLPAHDFYVRVGQKLITYLSAPTEE
                     GIAYKIDMQLRPSGKAGPLVSSLEAFRDYHRTSSQLWERQSLIKARYVAGNSSLGEVL
                     EKLAADFAYGSGLSKEGVGEIHHLRMRMERELARESDSQFNLKKGRGGIVDIEFLTQM
                     LQLAHGVHHPSLRCQATLKALKALHQQGILKKKEYATLADGYLFLRRLDHRLRLERDQ
                     SIDAFERDPGRLDGIARALGYESAVGRSGKNVARAGRKLLADYESRREKIRACYERFF
                     LGATDGLVQSALSNIEH"
BASE COUNT          693 a          922 c          854 g          754 t
ORIGIN      
        1 gtgcgcggtg tcaatgtcgt gggattaaag cgtgccggct ttgacgatcg ccggattcga
       61 gccatccgca acgctttccg cgtgctattc cgaaaaggac gcaacctttc gattgcgctc
      121 acagaggtag aggaaagcat gagggccgat gacgatgtca tgaccctcgt ggagtttatc
      181 aggtcgtcga aacgaggcgt ctgcttcggt ggttagcgcg taggagtagg agtaccggca
      241 gcgtagctct cgggctctgg agattccggc ggcgcaccga cgaagtaaat cggctcgatg
      301 ccttttatgt ggttgggcgt tactacatag tgcccgcgca cggccggata gattatgttg
      361 ctcgccacat cggttcttcc accagtcagc acatacccaa gtccgccggt caccagaccc
      421 aaacccgcat aggtgacctt ggcaggtatg tagaagacgc tagccagtgc cgacgcgaat
      481 ccgtacccga gttcctgact gacaggctgt tcggggggct gatccactgg agctgtcgtc
      541 tgcgcatgta tcggcgctga catgacggtc acaaaagaag ctagggcaat gcccttcgaa
      601 atgtgttttt tcatgaacgt gacgatatcg cacctcccgt gctactgtca ataatgaaat
      661 tctatagtgc ccatgaaaaa aatgaattcc gggcgcctgt aatcggacgg tcgggcattt
      721 accagatgtt cgcccgtgta acttcgcggg tgggaagttg atgatcctgt aaattaatgt
      781 tctatattgg acaaagcaga ttgaactaac ccgtcagttg ctcctaggaa gaaccgttcg
      841 tagcacgccc ggatcttctc gcgtcgtgac tcataatccg cgagcaactt gcggccggcc
      901 ctggcgacgt tcttaccact ccgacctact gcgctctcgt agcccaaagc gcgagcgatg
      961 ccgtcgagac ggccggggtc acgttcaaat gcatcaatcg attgatcgcg ctcgagcctg
     1021 agacggtgat ccaagcgcct caaaaacaag taaccatccg ccagcgttgc gtattccttt
     1081 ttcttgagga tgccctgctg atgtaatgcc tttaaagctt tcaaggttgc ctggcaccgc
     1141 agactgggat ggtgaacgcc atgagccagc tggagcattt gcgtcaaaaa ctcgatatcg
     1201 acgatgccgc cacggccctt cttcaagttg aactggctgt cgctttcgcg ggcgagctct
     1261 cgttccatgc gcattctcag gtgatggatc tcaccgacac cttccttcga gagacctgag
     1321 ccatacgcga aatccgcagc gagtttttcc aggacttcgc ctaagctcga gttaccggca
     1381 acataacgcg ccttgatcaa cgattgccgc tcccacagct gcgagctggt gcggtgataa
     1441 tcgcgaaaag cttccagaga gctgaccaga ggaccggctt tgcccgatgg acgtagctgc
     1501 atatcaattt tgtaagcgat gccctcttcg gttggcgccg aaagataagt gatcagtttt
     1561 tgccctacac gaacgtagaa atcatgagcc ggcagtttac cccgcacgcc gccactgctg
     1621 tgagcgtcgt cgtccgcgtc gtaaacgaaa accaggtcca agtcggaatt gtagtctagc
     1681 tcacggccgc ccatcttgcc catacccaaa acggcaaatc gaccttggga aatgatgccg
     1741 aaattttgtt gcagctcctc gagagtcagt tgcaacgcgc actccaccac ggcatcggcc
     1801 aaatctgaaa gctgctcaag gaccggttcg atgtcgatgg taccgccgag gtcgtggagc
     1861 ccaatcctga tgaactcttc ggttttggag cgccggattg cgttcagttt atcctcgaca
     1921 tcgtcctgct ccgctatggc ttcgcgcagc cctgccatca ttgactcttt gctcttatcg
     1981 atgcgcgtca gatccacgcg aatcagagtg tcgatgagct ctggacgagt taggaaaagc
     2041 tcggtgagga attggctgtc ggcaaacaga gtaatcagca gccgcatggt ttccgggttc
     2101 tccgccagca acctcagaaa cccgctgcgg ccgccgattc gatggctgaa tctcgacaaa
     2161 ttcaatagag cttggtcggg cgcgccggat ttggcgattt ccgcgatcaa cgccgggccg
     2221 agggtgcgca taatcttgag ccgtttggcg cttggcggag cgaagctctc gccgtcgcgg
     2281 accgccaaaa gattctcgta cgctttgctg gcatcggcga atccaagctt ctcgagttcg
     2341 cgaataatta gttcgcgctg atcgagatcg tgccaaatgg tgccggcgtg ggattggccc
     2401 tcatgagtaa tctctttttg cgccccataa aagagccggt cgaatatacg ccgcacattt
     2461 tcgctgtgaa tatttttctc cgaccagaac tgctcgcgct cgctgcgctc gcctttcggt
     2521 aagtacccga ggcgacgggc gagtgcttct tcttcttcgc cctcgggaat cgaatgagca
     2581 tgcgcttctt gcaccatctg cactttgtgc tcaacctggc gcagaaaacg ataagcttcg
     2641 gccaactggt tcatctcttc ggcggatatg aacttatgcg cggcaagctc ggacaaggcc
     2701 cgcagcgtgt tctgttgtcg cagagccggt tcatagcctc cgttaaccaa ttgcagcgcc
     2761 tgggcgaaaa attcgatctc acgtatgccg ccaaaaccga gcttgatatt tctctccttg
     2821 tcctgtgaat tcagcagctc gttttcgatc cgcgtcttca tatgccgaag ctcctcgacg
     2881 gtggtgtagt cgaggtagcg gcgatagacg aacggctcca cttccttcag aaaacgaacg
     2941 cctaattttt tgtctcccgc caccgcccgc gccttgatca aggcagcccg ctcccagcac
     3001 tgcccccagg attcgtaata cagcatggcc gagtccaccg attgcaccaa gggtccattc
     3061 acacccagag gtcgaagcct caagtcgatg cgaaagacaa acccgtcttc ggttactttt
     3121 cccatcgcct gaatgatttt cttgccgatg tcagcgaaga acgctctcgg ctcagccttg
     3181 ccctttgttc caccggaact ctcgccttcg tcattttcaa aga
//
LOCUS       DASUTG010000479         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_666830, whole genome shotgun sequence.
ACCESSION   DASUTG010000479 DASUTG010000000
VERSION     DASUTG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666830"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1971)
                     /gene="pheT"
                     /locus_tag="VF089_10930"
     CDS             complement(<1..1971)
                     /gene="pheT"
                     /locus_tag="VF089_10930"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6174520.1"
                     /translation="MKFTFTWLTELVELDAAPNELSDLLTMAGLEVESLTSLGGRDAD
                     TKDWVFEISVTPNRGDCLSVLGLAREVAALTGKPLKDSPGDSPVRVVQCLGTVGIEIV
                     NPELCPRYSAMVVENVRIGQSPKWMQQRLEACGFRPLNNVVDVTNYVMVEIGQPLHAF
                     DLDRLTSGQIVIRQSSGDKEFVALDNVKRELADGDLLICDGDTPIALAGIMGGKNSEV
                     TAATRRVLLESAHFDPTTIRRTAKRLGLHSEASHRFERGVDPAGTVSAANRAAQLISK
                     HADGSVSAGVLDSYPRPPQLPSILLRQERIERLLGVEIAPVEAERILTGLGLETKKGE
                     VEGTWRVVPPTSRCDLTREADLIEELARLYGYQKIPTTLPVLRPTTGKADYQLMWERR
                     LRSFLAGEGLFEVINLPFTNETLNRTFTGLWNRPASPVAVLNPLVKESAEMRLSLLPG
                     LIENLRLNLAQKADGFFAYHLGKVFRLRTNGEIEEKQCLGGLLYGSRMRKGLRSLDTP
                     GAVTFLDCKGIIEGILDLFHLRESIVWSRGNVAVLHPGRSAELLRCDKKVGYMGESHP
                     DVNDQFGLSPLMAFELDFGKLLQYSPRQIRASSLPRYPAVERDFAVVVDQTVPSQQII
                     TCINALGQNLIKTVELFDEYRGDSIPPGKKSLA"
     gene            complement(2054..>2862)
                     /gene="pheS"
                     /locus_tag="VF089_10935"
     CDS             complement(2054..>2862)
                     /gene="pheS"
                     /locus_tag="VF089_10935"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6174521.1"
                     /translation="VKARQKEKALREDSIDVTLPGNRWEPGHIHPLTIVIDEIIDVFW
                     GMGFEIARGPDIEDDYHNFEALNIPQDHPARDMQDTFFVAEGRLLRTHTSPVQIRTME
                     SRQPPLQVIVPGAVYRHDDDATHSPMFHQVEGFMVDRSIAFSDLKGVLTHFLHRMFSE
                     KTGVRFRPSFFPFTEPSAEIDIQCVICGHDREVAQQRHCRVCKGSGWLEILGAGMIDP
                     EVFKFVGYDPDRVSGFAFGMGVERIAMLKYSIDDIRLFFQNDFRFLRQFG"
BASE COUNT          702 a          827 c          713 g          620 t
ORIGIN      
        1 cgccaagctc ttcttgccag gaggaatcga atcgccgcga tactcgtcaa ataattctac
       61 tgtcttaatt agattctggc cgagtgcgtt gatacacgtg ataatctgct gagacggaac
      121 agtctgatcc acaacaaccg caaaatcacg ctcaaccgcc ggataacgcg gcaaggaact
      181 ggcccttatt tgccgcggag aatactgaag caactttcca aagtcaagtt cgaatgccat
      241 cagtggcgag aggccgaatt gatcattaac atccggatga ctttcgccca tgtagcctac
      301 cttcttatcg caacgtagca gttctgcgct gcgtccggga tgaagaacgg caacgttccc
      361 gcgcgaccaa acaatcgatt ctcgtaaatg gaacagatcg agaatccctt caatgattcc
      421 tttacagtca agaaaggtaa ccgccccggg cgtatccaac gagcgcaatc ctttgcgcat
      481 acgagaaccg tatagcagcc cccccagaca ttgcttctct tcaatctccc cgttggtccg
      541 caaacgaaag accttgccga gatgataggc aaaaaaccca tccgcctttt gcgcgagatt
      601 gagacgcaag ttttcgatca accccggcag caaactgagg cgcatctccg cactctcttt
      661 gacgagcgga ttcaagactg ccacaggaga ggcaggtcgg ttccataggc ctgtaaacgt
      721 gcgattcagt gtctcgtttg taaacggaag atttatcact tcgaacaagc cttcacccgc
      781 caaaaacgag cggagcctcc gttcccacat tagctgataa tcagccttgc ctgtagttgg
      841 acgcaagaca ggcaaggtcg tgggaatttt ctggtagcca taaaggcgcg ctaactcctc
      901 tatcagatcg gcttcccggg tcaaatcgca gcggctcgtc ggcggcacaa ctcgccaggt
      961 gccctcgact tcgcccttct ttgtttcgag accgagaccc gtcagaattc tctcagcctc
     1021 aaccggtgcg atttcaactc cgagtaatct ttcgatgcgt tcctgccgaa gtaaaatgga
     1081 cggaagttgc ggcgggcgcg ggtagctgtc gagaaccccg gcggaaacgg aaccgtcggc
     1141 atgcttagag atcagttgag cggcccggtt agcagccgat accgtcccag ctggatctac
     1201 accccgctcg aatcgatgcg atgcttcact gtgcaatccc aaacgttttg cggtgcgccg
     1261 aatcgtggtc ggatcaaaat gcgcgctctc caaaagcacc cgacgggttg cggcagtaac
     1321 ttcggaattc ttgccgccca tgataccggc taaggcgatc ggcgtgtcgc cgtcacagat
     1381 cagaagatcg ccgtccgcca gttctctctt cacgttatcg agtgcgacga attctttgtc
     1441 gccgctggac tgccggatca ctatctgacc gcttgtcaat cgatcgagat cgaaagcatg
     1501 cagcggttga ccaatctcca ccatcacgta atttgtcaca tcgacgacgt tattaagcgg
     1561 gcggaaaccg caggcttcga ggcgctgctg catccacttc gggctttgcc cgatacgaac
     1621 gttctcaacc accatggcag agtagcgtgg gcagagttcc ggattaacga tttcaatccc
     1681 aaccgttccc aggcactgga ccactcttac tggcgagtcg cccgggctat cctttagcgg
     1741 tttgcccgtg agcgcggcaa cttcgcgcgc caaaccgagg acgctcaggc aatcgccgcg
     1801 attgggcgtg acgctgattt cgaataccca gtctttggtg tcggcatccc gcccacccaa
     1861 cgaggtgagg gattcgactt caaggccagc catcgtcaac aaatcggaaa gttcgttggg
     1921 agcggcatct agctcgacca actccgtcaa ccatgtaaaa gtaaatttca tgaaaaccac
     1981 ataaaaggtc agggaggcga gcccaggatc tcgagcccaa gtcggtcaat gcgcagccat
     2041 cacttaagag gttttagccg aactgcctaa ggaatctaaa atcgttctgg aaaaataacc
     2101 ggatatcatc gatgctgtat ttgagcattg cgatgcgctc gacccccatg ccgaaagcga
     2161 accccgatac tcggtcggga tcgtaaccga caaacttgaa aacctcggga tcgatcatac
     2221 cggcgccaag gatctccaac cagccgctgc ctttgcaaac tctgcagtgc cgctgctgag
     2281 cgacttctcg gtcatggccg caaatcacgc attgaatatc gatctcggcg ctgggctctg
     2341 taaacgggaa gaagctcggg cgaaaccgca caccggtttt ctcgctaaac atacggtgca
     2401 aaaaatgtgt cagcacccct ttcaaatccg agaacgcgat gctgcgatcc accataaaac
     2461 cctcgacctg gtgaaacatc ggcgaatgag tagcgtcatc gtcgtggcga taaaccgcac
     2521 ctgggacgat cacctgcaat ggcggctggc ggctctccat ggtgcgaatc tgcaccggtg
     2581 aagtatgcgt cctcagcaat cgtccctcag caacaaaaaa agtatcctgc atatctcgtg
     2641 caggatggtc ctgcgggata ttcagcgctt cgaaattatg atagtcgtct tcgatatcag
     2701 gaccacgggc gatctcaaag cccattcccc agaaaacatc tataatttca tcgatgacaa
     2761 tcgtaagcgg atggatgtgg ccgggttccc agcgatttcc cggcagcgtc acatcaattg
     2821 aatcttcccg tagcgctttc tctttctggc gcgccttaac ga
//
LOCUS       DASUTG010000480         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_667260, whole genome shotgun sequence.
ACCESSION   DASUTG010000480 DASUTG010000000
VERSION     DASUTG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667260"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VF089_10940"
     CDS             complement(<1..527)
                     /locus_tag="VF089_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6174522.1"
                     /translation="MSRLKLTLACGDYDFLRPLINGEIQPQGIDLNVLTMPSPERHGR
                     MLRHEEFDVCELSLIAYLVSHDRGCNYTAIPVFPHRRFRHSYMVKRTGCGIDRPSDLN
                     GKRVALDTLQNSAGLWMRGILQDHYGVDLKSIEWWCQEEEDIAFEPAKWMNVKRVPPG
                     KNVDQMLLDGELEAAL"
     gene            complement(528..1118)
                     /locus_tag="VF089_10945"
     CDS             complement(528..1118)
                     /locus_tag="VF089_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6174523.1"
                     /translation="MIIAVVLSAGESSRMGRPKALLPIEGQRFIERIIRILGQSKVSR
                     TIVVLGHDADQLRGQLEHLPVEFVINPDYHSGQLSSLQAAIRHIERDDRCDGMLVHLV
                     DHPFIDVALVDKLIEGFFETRKLIVVPRYKEKRGHPVIFSRELFRELLDAPIDQGAKA
                     VVNAHRQETLEIEWQDEGITLDIDTPELYRQHVRGE"
     gene            complement(1136..2122)
                     /locus_tag="VF089_10950"
     CDS             complement(1136..2122)
                     /locus_tag="VF089_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174524.1"
                     /translation="MRILVKIALAVVFLLVLAAQSFSQERIKIAYSSADASNVVWFTA
                     LDAGLYRKHGLDVELVFIQSSTMSVSTLVSGDIQVANSSGGAVASAVVAGANLIMTAC
                     YINTLPYELVVQESVKSMEDLRGKSVGISRVGSASDVAARVLIKGLGLEPVKDVPIMQ
                     VGGPSERAAAFRTGKIVGFPSPPGTIHLAKGMPHRIMISTADFQKRFDFPYICSTTTK
                     SYLASNRETMRKLTMALIEATHFLKTRKQETMKFIAKYTRQDNPQFLESSWAANVKLH
                     DRVPLVTREGTEVQIKEALARKPGATLRVEDIVDDSIVRELEKSGFIDKVYK"
     gene            complement(2270..3370)
                     /locus_tag="VF089_10955"
     CDS             complement(2270..3370)
                     /locus_tag="VF089_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174525.1"
                     /translation="MIEVKSLVKIFADGKNAGVRAVDRVSFTVEEGRFYTLLGPSGCG
                     KTTTLRCIAGLEKADEGEIIVAGEKVFSASSRTFVPAYRRPIGMVFQSYAIWPHMTVF
                     ENVAFPLRVGKQKVSGDEIRRKVMTAVEQVELGGYEDRMATQLSGGQQQRLALARALV
                     REPKVLLLDEPLSNLDAKLRERMRVELRELQRRLGITTLYVTHDQIEALSMSNVIAVM
                     SAGVTVQEGTPREIYLQPRSKFVAQFIGSTNQISGQVTNRGSDGIGNVKTEEGEISCG
                     ILTELKAGDKVVVVVRPESIVLHQKKPAHGDNILEGKIGAAMFLGEYVDCTIELGKNV
                     LETHQPHSLRVRRGDTVWVELPVGDCMALPAE"
     gene            complement(3383..>3964)
                     /locus_tag="VF089_10960"
     CDS             complement(3383..>3964)
                     /locus_tag="VF089_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6174526.1"
                     /translation="MTATVAMVLTSLIAWIVYKSRLPGSWLLDFLAFIPITVPGIVMG
                     MALILLYVAFPLPIYGTIWLLMIAYVTRYMPYGMRSASGAIMQIHSELEEAASASGAS
                     WWETFKRVTLPLLRPGIAAGWIYICIVSFREFSTSVLLATGESRVLSILLFTMFEQGQ
                     VTIVAAIGILMIVVLLAIVAVFYKISGRFGIQT"
BASE COUNT          932 a         1127 c         1003 g          902 t
ORIGIN      
        1 agcgccgctt ccagctcgcc atcgagcagc atctgatcga cgtttttgcc gggcgggacg
       61 cgcttcacgt tcatccactt tgccggttcg aaagcaatgt cctcttcttc ctggcaccac
      121 cattcgatgg acttcagatc gacgccgtaa tgatcctgaa ggatgccgcg catccaaagc
      181 ccggcggagt tctgcaaggt atcgagagcc actctcttgc cgtttagatc tgaaggtctg
      241 tcaatgccgc agccggttct tttgaccatg taggagtgcc gaaagcgccg gtgcgggaaa
      301 accggaatgg cagtgtagtt gcagccgcga tcatgagaca ctaaataggc gatcagtgat
      361 agctcgcaga cgtcgaattc ttcatgccgg agcattcggc catgccgctc tggagagggc
      421 atggtgagta catttaaatc aataccctgc ggctggattt cgccattgat caatggccgc
      481 aagaaatcgt aatcgccgca agccagggta agtttcaatc tggacattta ttctcctcta
      541 acatgctgtc gatatagttc aggggtatcg atgtctagag tgatgccctc gtcctgccat
      601 tcaatttcca gggtctcttg gcgatgcgcg ttaaccaccg ccttggcgcc ctgatcaatg
      661 ggagcatcga gcaactccct gaatagttcg cgcgaaaaaa tgaccgggtg accgcgcttc
      721 tctttatagc gcggcaccac gattaacttt cttgtttcga agaagccctc gattagtttg
      781 tccaccagcg cgacgtctat gaacggatga tccactaaat gcaccagcat cccgtcgcat
      841 cgatcatccc tttcaatatg acgaatggcg gcttgcaagg acgataattg gccgctgtgg
      901 taatcgggat tgatcacgaa ttcgactggc aaatgctcga gttggccgcg caattgatcg
      961 gcatcatggc ccaggacaac gatggttcga cttaccttgc tttgccccag tatccggatg
     1021 atgcgttcga taaatctctg cccttcgatg ggcaacagtg ccttcgggcg gcccatgcgg
     1081 ctggactcgc ccgcagaaag aacaacggcg atgatcatgc gttgtttcgg tagaactact
     1141 tgtagacctt gtcgataaac ccgctctttt ccaactcgcg tacgatgcta tcgtcgacga
     1201 tatcttcgac acgcaaagta gccccgggct ttcgcgccag cgcttccttg atttgcacct
     1261 cggttccttc ccgggtgacc aatggaactc gatcgtgcaa cttcacgttg gctgcccagg
     1321 atgactctaa aaattgcgga ttatcctgac gcgtgtactt agcaatgaat ttcatggtct
     1381 cttgcttacg ggttttaaga aaatgggtcg cttcaatgag cgccatggtg agcttcctca
     1441 tggtttcccg gttgctcgcg agataactct ttgtggtggt agagcaaata taaggaaagt
     1501 cgaaacgctt ttgaaagtct gcggtactga tcataattcg atgcggcatg cctttggcga
     1561 ggtgaattgt tcctgggggt gatggaaagc cgacgatttt tcctgttcta aacgccgccg
     1621 ctcgctccga cggtccgcct acttgcatga ttggcacgtc cttcaccggc tccaaaccga
     1681 gtcctttgat cagtacccgg gccgccacat ccgaagcact ccccacgcga ctgatgccga
     1741 cgctcttccc ccgcaaatct tccatcgatt tgaccgactc ctgaaccacc agttcgtagg
     1801 gcaacgtatt gatgtagcat gcggtcatga tcaggttggc gccggctacg actgcactcg
     1861 ccaccgcgcc cccggagctg ttagcaactt gaatatcgcc tgagaccagg gtagagacgc
     1921 tcatggtcga gctttggatg aaaaccagtt cgacatcgag tccatgtttc ctgtacaggc
     1981 ccgcatcaag tgcggtgaac cacaccacat ttgatgcgtc ggctgaactg taggcgattt
     2041 tgatccgttc ttggctaaag gattgtgccg ccagaaccaa caagaaaaca actgccaacg
     2101 ctatcttaac gaggatcctc atcgtacctc cgatgcgcta aaaagttctc taccacacaa
     2161 gctttgcatg ggaaacaata acgaaagagt gggaagattg aatgatgagc aatgtagtag
     2221 gcgaaaccgg ggctgtcgaa ggaatgaggc cgtacgagca ccgaaacaat tactcggcag
     2281 gcagggccat acagtcgccc accggcaatt ccacccaaac agtatcgccg cggcgtactc
     2341 ggagcgaatg gggttggtga gtctctagga cattcttgcc gagctcgatg gtgcagtcga
     2401 catactcgcc gagaaacatc gcagcaccga tcttgccctc caggatattg tctccgtgag
     2461 cgggcttctt ttgatgcagg acaatgcttt ctgggcgcac cacgacgact accttgtcgc
     2521 cggctttcaa ttcagtcaaa atcccgcagg agatctcccc ctcctcggtt ttcacgttgc
     2581 cgattccatc gcttcctcgg tttgtgactt gcccgctgat ttgattggtc gagccgatga
     2641 actgcgcgac aaatttactc ctgggctgaa gatagatctc acgcggggtc ccttcctgaa
     2701 cagtcacccc cgcgctcatc actgcgatga cattggacat cgatagtgct tcgatttgat
     2761 catgcgtcac atacaaggtg gtgatgccga gccggcgctg caactcgcgc agctcgacgc
     2821 gcatgcgctc gcgcagcttc gcatccaggt tacttagcgg ctcatctagg agcaggacct
     2881 taggctcccg gaccaaggcg cgcgccaacg caagacgttg ctgctggcct ccggaaagct
     2941 gcgttgccat ccgatcttcg tagccgccca attcaacctg ctcaacggca gtcataacct
     3001 ttcggcggat ctcgtcgcca ctgaccttct gtttgcctac cctaagtgga aaggcgacat
     3061 tttcgaaaac cgtcatgtgc ggccaaatgg cgtaactctg aaaaaccatg ccgataggcc
     3121 ttcggtaagc cggaacaaag gtgcggctgg aggctgaaaa aaccttctct cccgccacta
     3181 tgatttcgcc ttcatcggcc ttttccaaac cggcgataca tctaagcgtc gtcgtcttgc
     3241 cgcaacccga gggtcctaac aaagtataaa accgtccctc ttcaacagtg aaagaaaccc
     3301 ggtcaaccgc cctcactccg gcgttcttac cgtcggcaaa gatcttaacc aatgacttta
     3361 cttcgatcac aactgctttc ccttatgtct gaatgccgaa acgtccagag attttgtaaa
     3421 aaaccgccac tatcgccagt aagacaacaa tcatcaagat gccgatggcc gcgacaatcg
     3481 tcacctgccc ttgctcgaac atggtaaaca gaagaatcga cagcacgcgg ctttccccag
     3541 ttgccagcaa cactgaagtc gagaactcgc gaaacgacac gatgcagatg taaatccaac
     3601 cggcggcaat gccgggccgg agaagcggca aagtcacgcg cttgaaggtc tcccaccagg
     3661 atgcgccgga tgccgacgcc gcttcttcca gttcgctatg gatctgcatg atcgctcccg
     3721 atgccgagcg catgccgtag ggcatatagc gggtcacata cgcgatcatc aacaaccaga
     3781 tcgttccgta gatcggcaag ggaaacgcga cataaagaag gatcaaggcc atacccatga
     3841 cgattccggg aacggtaatc ggtatgaaag ccaaaaaatc cagcaaccac gatccgggaa
     3901 gccggctctt gtagacgatc catgcaatga gagaggtcag aaccatcgcg accgtagcgg
     3961 tcat
//
LOCUS       DASUTG010000481         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1555482, whole genome shotgun sequence.
ACCESSION   DASUTG010000481 DASUTG010000000
VERSION     DASUTG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555482"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF089_10965"
     CDS             <1..234
                     /locus_tag="VF089_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEX6174527.1"
                     /translation="EFFDKFKDEFDYRYKIGADNPTMMNVISHAYLSARIPVIDTFEQ
                     IIAYAKRHKDVWFARRGDIAAWARKYFEENESR"
     gene            complement(346..2061)
                     /locus_tag="VF089_10970"
     CDS             complement(346..2061)
                     /locus_tag="VF089_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6174528.1"
                     /translation="MVRRFSLDGGKLALALIIAILVYQVVIPLLMVVWTSLKVERPGE
                     PGFFELSFSLANYARAFGSRDFWQATWNTLYFALASTAASFGFGTFLAWIVHRTNTPL
                     ARLIGVITLGRIIIPGILITVSWILLASPSIGVLNHALEGITGIKAILNVYSFWGMVW
                     VHSLEMTPLAYLLLSAALQSMDPRLEEASGAAGAGHWSTFRRVSLPLMLPAVGAAVML
                     LLIYTIETFEVPLLLGGRAQVRVYTTEIYFNTARTPTDWGLSGAYSIAVLFLCAVFLA
                     VYFRLVRHGERYQTVTGKDFRPRRIDLGRWRYLTCGLSLLLVFLITGLPFIVMLYASL
                     LRRYQPPSLAVLQSLSLENYHEILSDPIYSLDPMWNSTLVGLASATAVMLLVSIMSYF
                     VHKTRLRGRKLIDFLGFAPVAIPSVVLGTAFLWFYLLVPVPVLGTLTIICLAYITRYM
                     AVALRFVSTSMLQIHSELEEAAEVAGGRWWSNFRRIYLPLLRPGLMAGWFWVMVHAYR
                     ELTIALMLARSTNRTAAVVIYDLWENGSFPQLSAFGVLIFGLLIVLVLLGQTVGKRFG
                     VQEQV"
     gene            complement(2103..3206)
                     /locus_tag="VF089_10975"
     CDS             complement(2103..3206)
                     /locus_tag="VF089_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174529.1"
                     /translation="MKHFKMKDFKRCLRIALIVCAVATMSSADFAYAAASQSPEQLFA
                     ELAKLKPEQRGKRLEEGSRKEGKLNFIHTFRGKLAREHVRLFEKRYPFIKVEMADMGS
                     QDAAERFIAEETAGRHLTDTISLAVPDLPVILKQDLLAEYPTPATSRIPKQYEGFLDK
                     QNRWVPYYWSEHGISYNTNLIPPDKAPKSWQDLCNPAYKGQVSFDPPETRFMVGLYVL
                     MGEEKFQEWLKCMGQNQPIIQRGHTQRMNLMLSGDHAVQGDNFLYVGAEEKKKDPKTP
                     YAIVYSAPILAYAGVMIINKNTQSPHAAALFSDWTLSEESQKYIAAEFRGPLIGKHPY
                     LPDNIKVVTFSYVSDDIVDRLHGYWNQYVGKKK"
     gene            complement(3386..4852)
                     /gene="tcuA"
                     /locus_tag="VF089_10980"
     CDS             complement(3386..4852)
                     /gene="tcuA"
                     /locus_tag="VF089_10980"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6174530.1"
                     /translation="MRTETIPVVVVGGGSAAFEAAVAAKQAGAPRVVMLEKAPEPEFG
                     GNARFSHTGFRWVFSGAEEVRRFLPELDDQTFQKLHLPAYSAEQFTADLQRVTRGRID
                     KELANVMVQQSNAAMHWMRELGIKWEIDSHVMIDGRYYFEPGLVIHPVGGVGGGLGQL
                     MQWREIAMRMGIEIRYESRVKQLLGNDRRIEGVAVSDANEEYDIRTGSLILCAGGFQA
                     NPEMRARYLGANADLMKVRGSRHDTGEVLMMALALGCKASGHWQGAHATPIDSTFPDV
                     EIGSKANRYGYPYSITVNSLGQRFFDEGEARHSYTYAKTGWAVLGQPGAVAYQIYDQK
                     TIPLLGTRYEFSTPIVANTIHELAGKIGVEPAVLEHTVKEFNNAVRGDIPFDPTKLDG
                     KCTEGVMPKKSNWASPLEQPPYWAYSITGGITFTFGGLQINESAQALNTSGNPIHGLY
                     ASGDIVGLFFHNYPSCTGQTRNVVFSRLAGRHAAAQGL"
     gene            complement(5052..5450)
                     /locus_tag="VF089_10985"
     CDS             complement(5052..5450)
                     /locus_tag="VF089_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174531.1"
                     /translation="MPKLKHLAIRTEDTGKLATFYMDVFEMQVLHKDKNEGGAIFLTD
                     GYFNLAILPNHEQQAPNGLYHFGFEVESGENIVERMKKVNPNKLPKPRPNGRPYAETR
                     GSDPDGNYFDISEHGFSNVVPLGENKDKAN"
     gene            complement(5507..>5695)
                     /locus_tag="VF089_10990"
     CDS             complement(5507..>5695)
                     /locus_tag="VF089_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6174532.1"
                     /translation="FGGLEVNRQAQVLNTGGKPIRGLFASGDILGLFFHNYPSCTGQT
                     RNVVFSRLAGRHAAAQGL"
BASE COUNT         1346 a         1581 c         1561 g         1207 t
ORIGIN      
        1 gaattctttg ataaattcaa ggacgaattc gactatcgct acaaaatcgg cgcggacaat
       61 ccgacgatga tgaacgtaat ctctcacgct tatttgagcg cgcgcattcc cgtcatcgat
      121 accttcgaac agatcatcgc gtatgccaag aggcataaag atgtctggtt tgcccggcgc
      181 ggcgatatcg ccgcatgggc gagaaagtat tttgaagaga atgaatctcg ctagcaggtt
      241 gctgatgctt aaaacgactc agagcgcagc cggtcctcgt gctcgtgtcc gtgaaaacgt
      301 attcgagact tccgacaaaa acgaacaccg cttaagaagc tcgccttata cctgttcctg
      361 aacgccgaac cgtttgccga cggtctgtcc caacaagacc agaacgatca ataagccgaa
      421 gatcagcaca ccgaaagcgc tgagctgtgg gaacgaaccg ttttcccaga gatcgtagat
      481 gaccacggcc gctgttcggt tggtcgaacg cgccagcatc agtgcgatcg tgagctcgcg
      541 gtaggcgtgc accatcaccc aaaaccagcc ggccatgagt cccgggcgaa gcagcggcag
      601 atagatccgg cgaaaattgc tccaccagcg ccctccggcg acttccgcgg cctcttccag
      661 ctcgctatgg atctgcagca tcgatgtgga gacgaagcgc agcgccaccg ccatgtaccg
      721 ggtgatgtag gccagacaga tgattgtgag cgtaccaagg acgggcacgg gaaccagcaa
      781 gtaaaaccac aaaaaagccg tgcccagtac gacgctcgga atggcaaccg gtgcgaaacc
      841 gagaaagtcg atcagcttgc ggccgcgtaa gcgcgtcttg tggacaaaat aactcatgat
      901 cgaaaccaaa agcatgacag cggtcgccga ggccagtccc acgagagtgc tgttccacat
      961 tgggtccagg gaataaatcg gatcgctaag gatctcgtga tagttttcta gactcaaact
     1021 ctgcaacaca gcgagagaag gaggctgata acggcgcaag agcgaggcgt agagcatcac
     1081 gataaacggc aggccggtga tcaaaaaaac cagcaacaaa cttagcccac aggtgagata
     1141 gcgccagcgc cccaaatcga tgcgacgcgg gcggaagtct ttgccggtca ccgtctggta
     1201 gcgttcgcca tgtcgcacga gacgaaaata aacagccagg aaaaccgcgc acaggaacag
     1261 gaccgcgata gaataagcgc ccgagagccc ccaatccgtc ggcgttctgg cggtgttgaa
     1321 ataaatttca gtggtgtaga ccctgacctg ggcgcgccca ccgagtaaca gcggcacttc
     1381 gaaagtttca atggtataga tcagcaacag catgaccgcc gcacccaccg cgggcagcat
     1441 caacggcaga gagactcttc tgaaagttga ccagtgccct gcgccggccg cccctgacgc
     1501 ctcttcgaga cgcgggtcca tggattgaag cgccgcggag agaagcagat atgcaagcgg
     1561 ggtcatctcc agcgaatgga cccaaaccat cccccagaaa gagtaaacgt tcagtatcgc
     1621 tttaatcccg gtgattccct ctaaagcgtg gttcagaacg ccgatgctcg ggctcgccag
     1681 gaggatccac gatacggtga ttaagatccc gggaatgatg attcgcccga gggtaatgac
     1741 gccgatcagc cgggccagag gagtgttggt tcgatgcacg atccaggcaa gaaaagttcc
     1801 gaagccgaat gaagcagccg tggaagcgag agcaaagtag agggtgttcc aggtcgcctg
     1861 ccaaaaatct cgtgaaccga atgctcgagc ataattggcc agtgagaaac tcagctcaaa
     1921 gaaccccggt tccccggggc gttcgacctt gagactggtc caaacgacca tcagcagcgg
     1981 aatgacgacc tgataaacca ggattgcaat aatgagggcc agcgcgagtt tgccgccgtc
     2041 taacgaaaac cgccgcacca tcactgacga gccggttcca tatcaagcga tgtcaatcag
     2101 gattattttt tcttaccgac gtactgattc caataaccgt gtaagcggtc gacgatatcg
     2161 tcgctgacat agctgaaagt gacgaccttg atgttatccg gtagataggg atgcttgccg
     2221 attagcgggc cgcgaaattc agcggcaata tatttttggc tttcttcgga aagcgtccag
     2281 tcgctgaata gcgctgccgc atgcgggctc tgagtgttct tgttgatgat catcacgccg
     2341 gcatacgcca gaatcggcgc ggaataaacg atcgcgtagg gggtctttgg atctttcttc
     2401 ttttcctctg cgccaacata aaggaagtta tcgccctgaa ctgcatggtc gcccgatagc
     2461 atcagattca ttcgctgcgt gtgaccgcgc tgaataattg gttgattttg ccccatgcac
     2521 ttaagccact cctggaattt ctcctctccc atcaaaacat aaagtccgac catgaagcgg
     2581 gtctcgggag gatcgaagga gacctgacct ttgtacgcag gattacaaag gtcctgccag
     2641 ctcttgggcg ctttgtccgg cggtatgaga ttggtgttgt aggagatgcc gtgctcgctc
     2701 caataatacg gcacccagcg gttttgcttg tccagaaaac cttcatactg cttggggatt
     2761 ctgctggtgg ccggcgttgg atattcggcg agcaggtcct gtttcagaat gaccggcagg
     2821 tcgggaacgg caaggctaat cgtgtcggtg agatggcgtc ctgcggtctc ctcggcgatg
     2881 aagcgctccg ctgcatcctg agaacccata tcagccatct cgactttgat aaacggatag
     2941 cgcttctcga atagacggac atgctcgcgc gcgagcttgc ctctgaaggt atgaatgaaa
     3001 ttgagcttcc cctccttgcg agatccttct tcgaggcgct ttcctcgctg ctccggcttt
     3061 aacttggcga gctctgcaaa tagctgttcc ggagattggg aagcggcagc gtatgcaaaa
     3121 tcagccgaac tcatcgtcgc cacggcgcag acgataaggg caatcctcag acaacgtttg
     3181 aagtctttca ttttgaagtg tttcataatg tgggatcctt ctctcgagaa tcagcgttcc
     3241 taacccgtcg cgccatagga ggcctttgta tatgccaggc gatgcgcgaa tttcaacctg
     3301 gtgaacgtct ttggccaagg tattagacgg aatgtcgaga aagacctctg agcgaaacac
     3361 cggatcagag tggtaaccga aatcgttaca gcccctgggc tgcggcatgg cgtcccgcca
     3421 accgggagaa aacgacgttg cgtgtttgtc cggtgcaaga cggatagttg tgaaagaaca
     3481 gaccaacgat gtcgccggag gcatagagac cgtggatcgg gtttcctgaa gtattgagcg
     3541 cctgggcgct ctcgtttatc tgcaggccgc caaacgtgaa ggtgataccg ccggtgattg
     3601 aataggccca gtagggaggc tgctcgagag gactcgccca attggatttc ttcggcatga
     3661 cgccttcggt gcacttgccg tccaatttcg tcggatcgaa tggaatgtcc ccgcgcacgg
     3721 cgttgttgaa ctctttaact gtgtgttcga gcaccgctgg ctcaacaccg atcttgccgg
     3781 ccagttcatg aatcgtgttt gccacaatcg gcgtggaaaa ttcgtatctc gtcccgagca
     3841 acggaatcgt tttctgatcg tagatttgat acgccaccgc tccaggctgg ccgagcaccg
     3901 cccatcccgt cttggcatag gtataggaat ggcgcgcctc gccctcatcg aagaaacgct
     3961 gtcccaatga attaacggtg atgctgtagg ggtaaccgta gcgattggcc ttgctgccga
     4021 tctccacgtc cggaaaagtt gagtcgatcg gcgtggcatg ggcgccctgc cagtgccccg
     4081 aggctttgca ccccaaggct aacgccatca tcagcacctc gccggtatca tggcggctgc
     4141 cgcgcacctt catcagatcg gcgttggcgc cgagatagcg cgcgcgcatt tcagggttgg
     4201 cttgaaaccc gccggcgcaa agtatcaggg accccgtgcg gatgtcgtac tcttcgttgg
     4261 cgtcggagac cgcgacacct tctatgcgcc ggtcgttgcc aagcaactgc ttgacccgtg
     4321 attcatagcg gatttcgatg cccatccgca tcgcgatctc acgccattgc atcagttggc
     4381 ccaagccgcc gccgacacca ccgaccggat ggatcaccaa gccgggctca aaatagtagc
     4441 ggccatcaat cattacatgg ctgtcgatct cccacttgat cccgagctcg cgcatccaat
     4501 gcatggcagc gttggattgc tgcaccatca catttgctag ctccttatcg atccgtccgc
     4561 gggttacccg ctgcaaatcg gcggtgaact gctcggcgga ataggcaggc agatggagtt
     4621 tctgaaaagt ctgatcatcg agttccggca ggaatcgccg cacctcctcc gcgccggaga
     4681 agacccagcg aaagccggta tgtgaaaaac gcgcgttgcc gccaaactcg ggctccggcg
     4741 ctttttccaa catgacgact ctgggcgcgc ccgcctgttt cgccgccacc gccgcttcga
     4801 aagcggcgct accgccgccg acaacaacca ccggtatggt ttcagttctc atgataggaa
     4861 gctccgagca atgactcgaa tggatcgatt agcaattacg gcacctggcg aaacgccgta
     4921 tcacccccac taaatctcgc cttagcaaag gggcacaaag aggctgtcgc gattccgatt
     4981 cgcgctacct cggtctgtga tccgggttgc ccgagtaacg tcacgcaaaa tcagcgaact
     5041 tcgcgagcag cttaattagc tttgtcctta ttctcgccca gagggacgac attggaaaaa
     5101 ccgtgttcgg agatgtcgaa gtagttgccg tccggatccg aaccgcgggt ttctgcgtag
     5161 ggacggccat tggggcgcgg ctttggcagt ttgttaggat ttaccttctt cattctctcg
     5221 acgatattct caccgctctc aacttcgaaa ccaaaatgat agaggccgtt aggcgcctgc
     5281 tgttcatgat tcggtaagat cgccaaattg aaatagccgt cggtgagaaa gatcgcgccg
     5341 ccttcgttct tgtccttgtg cagcacctgc atttcgaaaa cgtccatgta aaaagtcgcg
     5401 agcttgccag tgtcttccgt gcgaatcgca agatgtttaa gtttcggcat ggttccaagc
     5461 ctccttatgt aaaatggcga atgactagct tcttgcccct gctcgatcac aagccttgcg
     5521 ccgccgcatg cctgccggcc aggcgcgaaa aaaccacgtt ccgtgtctga cctgtgcagg
     5581 atggataatt gtggaaaaac agtccgagaa tatctccgga agcaaatagc ccgcgaatag
     5641 gcttgccccc ggtgttaagc acttgcgcct gccgatttac ttcaaggccg ccgaa
//
LOCUS       DASUTG010000482         7709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_814859, whole genome shotgun sequence.
ACCESSION   DASUTG010000482 DASUTG010000000
VERSION     DASUTG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814859"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VF089_10995"
     CDS             complement(<1..705)
                     /locus_tag="VF089_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174533.1"
                     /translation="MTLGKLTRRAAALALVLAAVPFVPGELFAQKIKVGYWTSGFSLG
                     FGAVLEQMKLAEKEGLEVEWVKFAEVNGPTRAIVSNAIDLAFAAPSTNSIGIAADGVP
                     VKIVLATQIAEAQVVVLDGGPIKTVADLKGKKIGMSPPGSATHAISTAILDQNYGIKP
                     KEYSVAPGNEARLAQFLTQKEIDAAIIRSVTIAQMEETKLRRLASIVDEWKKLTKSSA
                     APILGVTIVYNDYLTKN"
     gene            complement(859..1653)
                     /locus_tag="VF089_11000"
     CDS             complement(859..1653)
                     /locus_tag="VF089_11000"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174534.1"
                     /translation="MDTPSFAQQECIEPGQPAAAIRLSGISKSFSAGETRLEVLKDVN
                     LTVADQAIVGLLGASGSGKSTLLNIISGIVKPDRGQVCLCGAPSAEFNDWRTISYMFQ
                     DDRLLPWRNALHNVEFALEAGSMPKPERIRRATEALELVDLKGFEASFPYQLSGGMRS
                     RVALARSLVTEPRILLMDEPFSRLDAQTRSLMHGELLRIHEIKRMTVVFVTHDVEEAV
                     ILADQVAVMSPRPGTIREIVSITLARPRDTIQAEVANYIQKLRALI"
     gene            complement(1728..2552)
                     /locus_tag="VF089_11005"
     CDS             complement(1728..2552)
                     /locus_tag="VF089_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174535.1"
                     /translation="MKDEMTEAVADSAWTRSNSRRLRFDLLLQRVVLVASLIGIWWLF
                     SLSVPHYILPGPARVWQALMLIAANGDLWSNLGITLWRVSVGFIIAALVSLPFGIFLG
                     ANKRAGEFFEPVIPVMNTVSSAIWAIFAIIWFGISNATTIFVVFMTAMPLIITNVWQG
                     TRTVNAEFIELAQVLRMPKWKVMTKIYLPTILPYFFSGARLAFGFGWRVSLVAETIGA
                     SSGVGYRLRQAADLIQTDQVFAWTLTLVVMMATLEMGLLRPLENHLFRWKKEAEAQ"
     gene            3374..4435
                     /locus_tag="VF089_11010"
     CDS             3374..4435
                     /locus_tag="VF089_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174536.1"
                     /translation="MRHNKLIAWLANSVLLALLLIGAPLYAQETFYRGKTIRFIVAYE
                     PGGGFDVYTRTIARHFGKHVPGNPTTIVENMTGAVGMIAANYIYNQAKPDGLTIGHFV
                     GTLILQHVLGNEAAKFDGRKFGWLGVPIADHGVCALTKASGISTLDGWLAAKEPVKMG
                     GHGPGGVTSDTARILRDALGLPLRVIDGYKCTGPIRLAAESGEVSGGCWAWESMKPTW
                     AQGFETGQVKVVLQTALKTHPELKDVPVAMNYAKTKEARQLLEVLDHAYGTSFRSYSV
                     PPGTPKDRLAILQKAFLDTLKDPEFLIDAKKAKLDIAPIDGAATAKTLAGFYDLEPPL
                     IAKLKKVLLSDVSGRETPQ"
     gene            4432..5271
                     /locus_tag="VF089_11015"
     CDS             4432..5271
                     /locus_tag="VF089_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6174537.1"
                     /translation="MTTIQTQLLSFKTADNETLHGLLFTAAAKSADLALLFVHGVAMN
                     FYLPPLAIFGQALAERGYHGFVINTRGHDWVNRAGDLTAFGGATYETFEDSTMDLDGA
                     LECLTRQGYKRFILIGHSLGCIKSLMYQGSRRRRDVVGVVSCSCPKQFYSARAMEQPE
                     FKQLMAQAEEMVAQGKGEEIIWAPASGAMGMFCARTYVNKYGKHENNDVRPHAGRLGC
                     PHLTIAGGAEHPFFPEYAKELAEASGANGSYKIVPGSNHFYDKHEPEVIEMITRWLEQ
                     FAA"
     gene            5384..6520
                     /locus_tag="VF089_11020"
     CDS             5384..6520
                     /locus_tag="VF089_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174538.1"
                     /translation="MNHFIVANENHRKHRGAPDYQELKFRNHWKGIKQMNCRSIKFCV
                     IVCSFLGLQLSLHIPVHSAAAPVKTVIGYATVTARLMPLWIAHEQGIFAKYGIDAQPI
                     LIRGAPTLVAGLASGDIHIGRTGGSAMLAAVAAGHDFKFVASFSSRNTYDLVVRPNIK
                     RAEDLRGKRFAVTSIGGTTWMGILLWLEHLGLDQQRDQIQMQVVGDQGIQAQAVENGL
                     CDAAVLDGAFSRKLQQKGMTILHEYTEMTKLLAGQSMVVPGALLKQKSDLAENYLKAE
                     IEALAYSLAPKNKPAVIKSLMRHLRTDAASAEDGYLDLTRGVDRKPIPSVEGLRNVQR
                     LLKTRTPKVGDLKVEDVIDGRIARRLDESGFIERTFASYGASLK"
     gene            6552..7583
                     /locus_tag="VF089_11025"
     CDS             6552..7583
                     /locus_tag="VF089_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174539.1"
                     /translation="MRRKIRRATFLTMLLVPLIAAASVYSQDTFFKGKTMRIIVGGPP
                     GGGFDTYARMIARQMSKHIPGEPTIIVENMPGAGMMIAANHIYKAAKPDGLTIGHFTG
                     SQTISQILGQPGVEFDMRKFTYLGAPTADHFSCAFTKASGIVSTDKWLASPRPVKMGG
                     IGPGNLTDNTLRVLSTATNFPIQLVSGYKGTAPVRLAMEAGELDGSCWGWDSIKSTWN
                     RAMETGDGIVVIQVAPKPHPDLPKVPLAINFGKNEEGKQLIDVVIHGGNIVERSFMLP
                     PGVPKERIGTIKSAFDKTLRDPEFLAEAKKARLGVAPISGDELTRRVNAFFKLPEDMK
                     TKIKRVLYG"
BASE COUNT         1842 a         2145 c         2062 g         1660 t
ORIGIN      
        1 attcttcgtc aggtaatcgt tatacacaat cgtgacgcca agaatcggcg cggcgctgct
       61 cttggtgagc tttttccatt cgtcgacaat actcgcgagc cggcgcaact tggtttcctc
      121 catctgcgca atcgtcaccg agcggatgat cgccgcatcg atttcttttt gcgtcaggaa
      181 ctgcgccagc cgcgcttcat tgcccggcgc gacgctgtat tctttcggtt tgatgccgta
      241 gttctgatcg agaatagcgg tgctgatggc gtgagtcgcg ctgccgggcg gcgacatacc
      301 gattttcttg cccttgagat cggcaaccgt tttaatcggg ccgccgtcga gcacaaccac
      361 ctgtgcctcg gcgatctgag tcgccaaaac gatcttcacc ggcacgccgt cagcggcgat
      421 gccgatggag ttggtcgaag gcgcggcaaa agcaaggtcg atggcattgg agacgatcgc
      481 gcgagtgggt ccgttaactt cggcaaactt cacccattcg acctcgagcc cctctttctc
      541 ggccaatttc atttgctcca aaactgcgcc aaatcccaag ctgaacccgc tggtccagta
      601 accaacttta attttttgag cgaacagctc gcctggcacg aaaggcacgg ccgcgagtac
      661 gagtgcaaga gcagcagcgc gcctagttag ttttcctaat gtcacgttac ctccgttttc
      721 tggggagcgc gttcaaccgc tcgcaccgct cgctattcaa cggttgcgga actctgacgt
      781 tgaacgtttg aataggccgc aggccggttg aaccgttgaa caatttttta ccagagaaac
      841 accccccgcc gatcgttttt agataagcgc cctcaatttt tgtatatagt ttgcgacttc
      901 cgcttggatc gtgtcgcgcg ggcgcgccag ggttatcgag acaatttccc ggatagttcc
      961 aggccgggga gacatcaccg ccacttgatc ggcgagaatc acagcttcct ccacatcgtg
     1021 cgtaacgaat accaccgtca tgcgtttgat ctcatgtatg cgcagcagct cgccatgcat
     1081 gagcgagcgg gtctgcgcgt caagccggga aaagggttcg tccataagca agatgcgtgg
     1141 ctcggtgacc aggcttcgcg ctaaggcgac acggctacgc atgccgccgg agagctgata
     1201 cggaaacgat gcttcaaaac ctttcaaatc gaccagctcc aaggcctcgg tggctcgccg
     1261 gattctttca ggtttcggca tcgatccggc ttctagagcg aattcaacat tgtgcagcgc
     1321 attgcgccat ggcaacaatc gatcgtcctg aaacatataa ctgattgtcc gccagtcgtt
     1381 gaattccgcg ctcggggcgc cgcagagaca tacctggcca cggtccggtt ttacgatgcc
     1441 ggagatgata ttgagcaagg tgctcttgcc tgagcccgag gcgccaagca atccgacaat
     1501 cgcctgatcg gctaccgtaa gatttacatc cttaagcact tccaaccggg tctccccagc
     1561 agaaaaactt ttgctgatgc cgctcaggcg gatggccgcg gcgggctgcc ctggttcgat
     1621 gcactcttgc tgagcgaaac tcggagtatc catatgacct caattagggg ctgatcgcgg
     1681 tcctcgggca gcagggtgcg tcctcctgga acacaatcgg ctgttcctca ttgcgcttcc
     1741 gcttctttct tccagcgaaa caggtgattc tccaacggcc ttagcaatcc catctccagg
     1801 gtggccatca tcacgaccag agttagagtc caggcaaata cctgatcggt ctggatcaga
     1861 tctgccgcct ggcgcaaccg atagccgacc ccgctcgacg cgccgatggt ttcggcaacc
     1921 aatgagacgc gccagccgaa cccaaacgcc aggcgcgcgc cggagaaaaa ataaggaaga
     1981 atcgtcggca ggtagatttt ggtcatcacc ttccatttcg gcatgcgtag cacctgagcc
     2041 aattcgatga actccgcgtt caccgtgcgc gtaccctgcc agacgttggt aatgatcaat
     2101 ggcatcgcag tcatgaagac aacgaaaata gtcgtcgcgt tggagatgcc gaaccagata
     2161 atggcaaaga tggcccagat cgcagaagac accgtgttca tgaccgggat taccggctca
     2221 aagaattcgc ccgcccgttt gttcgccccc aggaagatgc cgaagggcaa acttacaagg
     2281 gcggcaatga taaatcccac agagacgcgc cagagggtga taccgagatt gctccacaga
     2341 tcgccgttgg cggcgataag catgagcgcc tgccatactc gagccgggcc aggcaaaatg
     2401 taatgcggca cggacaaaga gaacagccac cagatgccaa tcaacgatgc gacgagaacg
     2461 acacgctgaa gaagaaggtc aaagcgcagc ctgcgggagt tggaccgcgt ccaagcgcta
     2521 tctgcaactg cctcggtcat ttcgtctttc atccgttctc ttaaagcagg cacgctctac
     2581 gatctgccta tcacagtttc tcgtggcgcc aaatctcgct atgcttccta ttgagaacaa
     2641 tgacgagcac cgtcaactaa gatacctgca tagaatacta ttgagagaaa ttcgtctctt
     2701 ggagaacaaa gttggctgtt aagagaagta gacctttcat cgccaaaaca ggcgcacacc
     2761 gcggatccat ccaattggct gttcaacggg agtttcttta ggaagccata gcagtcacag
     2821 acggcgcggc agcggcaaat cggcgcagcc tattcgcacg agttccccct aacgtgtgtc
     2881 ttacctgtga agcttccgac acatatttac agatgaactc gcagtcaagt ttttaatcag
     2941 gcttgtagtt gagaaactat tgtagacact ctaccgctcg caatcccgcc gcgcgcaagg
     3001 tcggtgcaac cgatggtgcg aggtcgcgca tataggctcg gtcatctcga atcacaccgg
     3061 ctatgcgcaa agttttcgtt tgctcgcggc agatgattcc atgtataaat cgggccaagt
     3121 cgcggcatcc aaagcactga cccgttctaa ctctggcaga tatggagaat accttagacg
     3181 tattagatat ctcgatctat ctctttatat ggacgggtca atcaacttac aacgccgctg
     3241 agagagctcc cggagagtcg gcgagctcaa gatggaggat tacgtcgatc aagtatcgtg
     3301 cagagtgatt ggcgctaaag aagaaggaca taacgaaact tgacttctta tcttttgcag
     3361 tagaggagcc cccatgaggc ataataaatt aatcgcatgg ctagccaatt cggtgctttt
     3421 ggcgctgctc ctgatcggcg cgccacttta cgctcaagaa acgttttaca gagggaagac
     3481 catccgtttt attgtcgcat acgagcccgg gggcggcttc gacgtttata cccgcacaat
     3541 tgcgcgccac tttggcaaac atgtgccagg aaatcccacc actatcgtgg agaatatgac
     3601 tggcgcggtc ggcatgatcg cagccaatta tatttacaac caagccaagc ccgacgggct
     3661 gactatcggt catttcgtcg gcacgttgat cttgcagcat gtgctgggca atgaagcagc
     3721 aaaattcgat ggacgcaaat tcggttggct aggcgtgcct atagctgatc acggcgtttg
     3781 cgctctgaca aaagcgagcg gcatatcgac tctggatggc tggcttgctg ccaaggaacc
     3841 ggtgaagatg ggcggccacg gacccggcgg ggtaacatcc gacacagcgc gcattttgcg
     3901 cgacgctctt ggcttaccgc ttcgggtaat tgacggctac aaatgcaccg gcccgatccg
     3961 tcttgctgcc gaatcaggcg aagtctcggg cggttgctgg gcttgggagt ctatgaaacc
     4021 gacctgggct caggggtttg agaccggcca ggtaaaagta gtcctgcaaa cagctctcaa
     4081 gactcatccc gaactgaagg atgttcctgt cgctatgaac tatgcaaaga ctaaggaagc
     4141 gcgtcagttg ttggaagtcc tcgatcatgc ttacggcacc agcttccgca gctactcagt
     4201 gccaccgggg accccgaaag accggctggc gatactacaa aaagcttttc tcgatacctt
     4261 gaaggacccg gaatttctta tagatgcgaa aaaagctaaa ctcgacattg ctcccatcga
     4321 cggtgccgca accgccaaga ccctcgccgg tttctatgat ctcgaacctc cgctaatcgc
     4381 taaactcaag aaagtcctac tatctgatgt gagcggcaga gagacaccgc aatgacgacg
     4441 atccagaccc agctgcttag ttttaaaacc gccgacaacg aaactctgca cggtctgctc
     4501 ttcacagccg ccgctaaatc ggcggatctc gcattgctct tcgtccacgg cgtagcgatg
     4561 aatttttatc tgccgcctct cgctattttc gggcaagcac tggccgagcg cggctatcac
     4621 ggcttcgtca tcaacacccg gggccacgac tgggtaaacc gcgcgggaga cttgaccgcc
     4681 ttcggcggtg cgacctatga aactttcgag gacagcacga tggatctcga tggtgcgttg
     4741 gagtgtctaa cccggcaagg ctacaagcga ttcattctaa tcggtcacag cttgggttgc
     4801 atcaaatcgc ttatgtatca gggcagccgc cggcggcgcg atgtcgtggg cgtcgtgtct
     4861 tgctcttgcc ctaaacaatt ctattcagcg cgagcaatgg aacagccgga attcaagcag
     4921 ctgatggcgc aggcggaaga aatggtggcg caaggcaaag gagaagaaat catttgggcg
     4981 ccggcgagtg gagccatggg aatgttttgc gcacgcacct acgtcaataa atacggcaag
     5041 cacgagaaca acgacgtgcg gcctcacgcc ggccgcctgg gctgtcctca tctcactatc
     5101 gccggcggcg cggaacatcc attttttccg gaatacgcta aagagcttgc cgaggcgagc
     5161 ggcgcaaacg gtagttacaa aatcgtccct ggatcaaatc acttctacga caaacacgaa
     5221 ccggaggtga tcgagatgat cacgcggtgg ctggaacaat tcgcagcttg atgcggctat
     5281 ctcgccttca gtaataaatc gcagggtcta tagcctctct tcttgcgttc atttacaacg
     5341 caagatgaca tgacgacata aaaatccgca aactcgtcct cgaatgaatc atttcatagt
     5401 cgccaacgag aatcatcgga aacatcgcgg ggctcccgac tatcaagaat tgaagtttcg
     5461 caatcattgg aaaggaataa aacagatgaa ctgtcgctcc atcaagttct gtgtaattgt
     5521 atgttctttt ctcgggctgc aactctcgtt gcatattccc gtgcacagcg ctgccgcacc
     5581 ggtgaaaacc gtcatcggct atgcaactgt caccgcgcgc ttgatgccgc tctggattgc
     5641 tcacgagcag ggaatctttg ccaagtacgg catcgatgcg cagccgatcc tgatccgcgg
     5701 cgcgcccacc ctggtagccg ggctcgcctc cggtgatata catatcggcc gaaccggtgg
     5761 cagcgccatg ctggcggccg tcgccgcggg acacgatttt aaattcgtcg ccagtttttc
     5821 cagccgcaac acctatgatc tggtcgtccg gcccaacatc aagcgagctg aagatttgcg
     5881 cggcaaacgt ttcgcggtga ccagcatcgg cggcaccaca tggatgggta ttctcctttg
     5941 gcttgaacat ctcggcctgg accagcagcg cgatcaaatc caaatgcagg tcgttggcga
     6001 ccagggcatc caagcgcaag cggttgagaa tggcctctgc gacgccgcgg ttctcgacgg
     6061 cgcgttcagc cgcaaactcc agcaaaaagg catgacgatt ttgcacgaat ataccgaaat
     6121 gacgaaactt ctcgccggcc aatcgatggt ggtccctggc gctctgctta agcagaaaag
     6181 cgatctcgcg gagaattact tgaaagccga aattgaagcg ttggcctatt ctctcgcgcc
     6241 gaagaacaaa ccggcagtta taaaatcatt gatgcgccac ctgaggactg acgccgcaag
     6301 cgcggaagat ggatatctcg atctaacgcg cggcgtcgat cgcaagccga tcccttctgt
     6361 ggaagggctg cgcaatgtgc agcggcttct aaaaacccgc acccccaaag taggcgatct
     6421 taaagtcgag gacgttatcg acggtcgcat cgcacggaga ctcgatgaaa gcggcttcat
     6481 cgaacgcact ttcgcctcct acggcgccag cttgaaatga atcgagtagc aatttaacgg
     6541 aggtaaactt gatgagacga aagattcgac gagcaacttt tttgacgatg cttttggtgc
     6601 cgctcattgc cgcagcaagc gtctattcgc aagatacatt cttcaaaggc aagaccatgc
     6661 gtatcatcgt cggcggccct ccgggcggcg gttttgatac ttatgcgcgc atgatcgcgc
     6721 ggcaaatgtc caagcatatt cccggtgaac ccaccatcat cgtcgaaaac atgccgggcg
     6781 ccggcatgat gatcgcagcc aatcacattt acaaagcagc caagccggat gggctcacca
     6841 tcggccactt caccggcagc cagaccatta gccaaatact gggccagccg ggcgtcgaat
     6901 tcgacatgcg caaatttacc tacctgggcg cgcctaccgc ggaccatttt tcctgcgctt
     6961 ttaccaaggc aagcggcatt gtcagcacgg ataaatggct cgcctcgcca cgaccggtga
     7021 agatgggcgg gattggacct ggaaacctga ccgataatac tttgcgagtt ctcagcactg
     7081 cgaccaactt tccgatccaa ctggtgtccg gatacaaagg tacggcgccg gtgcgactag
     7141 caatggaagc cggtgagctc gatggttctt gctggggctg ggattcgatc aagtcgactt
     7201 ggaatagagc catggaaacg ggtgatggca tcgtggtcat tcaagtggcg ccgaaacctc
     7261 accccgatct gcccaaagtt ccgctggcga tcaatttcgg caagaacgaa gaaggcaaac
     7321 agctaatcga tgtcgttatc catggcggca atatcgtcga gcgctcgttc atgcttcctc
     7381 cgggagtgcc caaagagagg atcggcacca tcaaatcggc atttgataag acgttgcgcg
     7441 atccggagtt tctcgccgaa gccaagaaag ccaggctcgg ggtggcgccg atatccggcg
     7501 atgaattgac ccggcgcgtg aacgcattct tcaagctgcc tgaagatatg aagacgaaaa
     7561 tcaagcgagt gctgtacggc tgatcagccg ggaggctaag gttctacagt tgaaagtcac
     7621 ttggagtctc ggtaggcccg gactaattca aggtagaagg tacaatacac ccagcttcga
     7681 ggtttagtat gttggcctaa ggccaaccc
//
LOCUS       DASUTG010000483         5273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1408887, whole genome shotgun sequence.
ACCESSION   DASUTG010000483 DASUTG010000000
VERSION     DASUTG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408887"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VF089_11030"
     CDS             complement(<1..725)
                     /locus_tag="VF089_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6174540.1"
                     /translation="MQQEHREIIQVEDLTVTYNGHLILDHVSFTVAAGEVFVILGGSG
                     SGKSTLLKHLIGLYKPAAGRIFIDGEDIIAAEGEDRLAILRKIGVMYQSSALFGSMTV
                     LENVCLPLEEFTALPSEAIRVIALIKLKQVGLESFGSHMPSELSGGMQKRAAIARAMA
                     LDPRVLFLDEPSAGLDPITSAELDELILSLARTLKITFVIVSHELSSIFTIADRVIML
                     DKQSKGIVATGNPHELRDQSDNP"
     gene            complement(734..1867)
                     /locus_tag="VF089_11035"
     CDS             complement(734..1867)
                     /locus_tag="VF089_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX6174541.1"
                     /translation="MDSPSHFEFKTRKTEADALEIIIKGRLDSFTTSRVWRRARDTVA
                     AAKVSTVSLDASGVDYCDGSGVALLVQMRDQQIRSGGYFEIRGLQPELQALVEDWQPV
                     DRAVAGRQDEQATSVVEEIGRSIVGIWQDIRSLVIFVGELASALARAGLKPRAARWRD
                     TLRIAESVGVNAVPIVALIGFLMGLIMAFQAAIPLGQFGAQIFVANLIGLAILRELGP
                     LMTAIVLAGRSGSAFAAELGTMKVREELDALKTMGLEPVPFLVVPRVIAAVLMTPLLT
                     IFADFVGLIGGAVVLISLGFPLITFWNQVQLAVGYGDLLGGLAKSFVFGILVAAIGCL
                     RGLQTQTGASAVGESTTRAVVSGIILIVIADGVFSVIYYYLGI"
     gene            complement(2201..3256)
                     /locus_tag="VF089_11040"
     CDS             complement(2201..3256)
                     /locus_tag="VF089_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX6174542.1"
                     /translation="MKSPSPVRGRGSITNPKNRFESIERIPELSDYCDEISSAQTQFF
                     ADDSRSLVAYNDSPDVGFKASVNPYRGCEHGCVYCYARPTHEYLGFSSGLDFETKIMV
                     KEDAPELLRSEFSSHKWKPQIIALSGNTDCYQPVEKKKQLTRRCLEVFLEFRNPVVII
                     TKNHLVTRDIDLLSELAKYQCIGVTISITTLDNKLASLMEPRASTPERRLAAIRTLSE
                     AGVPVGYLQAPMIPALTDSEAPAIAQAAAKAGAKFCGYVALRLPFAVKLLFEQWLEQH
                     YPGKKEKVLNRIRSMRAGKLNDPNFKSRMRGEGIFADQMAELFQLAQKKAGIRDRWPQ
                     FSVEHFRRTSVDQLSLF"
     gene            complement(3261..3494)
                     /locus_tag="VF089_11045"
     CDS             complement(3261..3494)
                     /locus_tag="VF089_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174543.1"
                     /translation="MDVSALPNIKLSIRQLKKEKAEILTSSGDSSKVKRIRRKIKLLK
                     RQTRDLAKQKKKLAAESANKGSAEKTGAAAAGE"
     gene            complement(4045..4160)
                     /gene="rrf"
                     /locus_tag="VF089_11050"
     rRNA            complement(4045..4160)
                     /gene="rrf"
                     /locus_tag="VF089_11050"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4255..>5273)
                     /locus_tag="VF089_11055"
     rRNA            complement(4255..>5273)
                     /locus_tag="VF089_11055"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1272 a         1464 c         1289 g         1248 t
ORIGIN      
        1 catggattat cgctctgatc gcgcagctcg tgtggatttc cggtcgccac gattcctttt
       61 gactgtttat ccaacataat aactcgatcc gcaatcgtaa agatactaga tagctcatga
      121 ctgacgatca caaaggttat cttcagagtt cttgccagac tcaaaatcag ctcatccagt
      181 tcagccgaag tgatcgggtc tagaccggct gagggctcgt cgagaaacag cacccttggg
      241 tcaagtgcca tcgcgcgggc gatcgccgcc cgtttctgca tgccaccgct taattctgac
      301 ggcatatggc tgccgaaact ttcgagaccg acctgtttca gttttatcaa agcgataact
      361 ctgatcgcct cactcggcag tgccgtgaat tcttccagcg gcagacaaac gttttctaga
      421 accgtcatcg aaccgaacaa tgcgctgctc tgatacatga ccccgatttt tcttaggata
      481 gcgagtcgat cttcaccttc agcggcaatg atatcttctc catcgatgaa gatgcggccg
      541 gcggcaggtt tgtagagacc tatcaaatgt ttgagcagcg tgcttttccc cgatccggag
      601 ccgcccagaa tcacaaacac ctcaccggca gcaacggtaa aacttacatg atcgagaatc
      661 aaatggccgt tgtatgtcac tgtaagatct tcaacctgaa taatctccct gtgctcttgt
      721 tgcatgttga gtgctagatg ccgagataat agtagatcac cgaaaaaaca ccgtcggcga
      781 tgacgatcaa gataattccg ctcaccacag cacgcgtcgt cgattccccg actgcactgg
      841 cacctgtttg ggtctgcagg ccgcgcaggc agccgattgc agccacgaga atgccgaaca
      901 cgaatgattt cgccaggccg ccgagcagat caccataacc aactgccagt tggacctgat
      961 tccagaaagt gatcaaagga aatcccagcg aaattaacac caccgcgcct cctatcaacc
     1021 cgacgaaatc ggcgaagatc gtgagcagcg gcgtcatgag aactgcagcg atcactcgcg
     1081 gcacgaccag aaaagggact ggctccaatc ccatcgtttt caaggcgtcg agttcctcgc
     1141 gaaccttcat ggttcccaat tccgctgcaa acgcggagcc cgaacgtccc gccaagacga
     1201 tggcggtcat cagtggacca agctcgcgca ggattgcgag accaattagg ttcgcaacaa
     1261 atatctgtgc gccaaactgc cccagcggta tcgccgcctg aaacgccata atcaaaccca
     1321 tgagaaatcc tatcaacgcg acaatcggca cagcgttgac tccaaccgac tcggcgatac
     1381 gcaaggtgtc tcgccatcga gcagctcgcg gcttgagacc ggcacgggcc agagccgatg
     1441 ccagctcacc gacaaatatg accaaactgc gaatgtcctg ccagattcca acgatcgagc
     1501 ggccaatctc ttcgaccacc gaggttgctt gttcatcctg ccgccctgca acagctcgat
     1561 ccaccggttg ccaatcttct acgagcgctt ggagctcagg ctgcagccct cggatctcaa
     1621 agtatcctcc cgacctgatt tgctgatcac gcatttgaac taacagagct actccggaac
     1681 cgtcgcagta atcgactccc gaagcatcca acgagactgt agaaactttg gcggcggcaa
     1741 cggtgtccct tgctctgcgc cagaccctgc tagtggtaaa ggaatccaac cggcccttga
     1801 tgattatttc aagcgcatca gcttcggttt tcctcgtctt gaactcgaag tggctaggcg
     1861 aatccatgat gggttaaagc agattcctat tatgctgcgg ttgatgcaac ggtcaacggg
     1921 cagcattaga gtgtaggatt ctctagctat gccattgaaa accgatttcg ttgtctgatt
     1981 ggggtttaag accttgtgtg tactgtcaaa aaacgtactt cccaggttca cattgaagca
     2041 agcgacaacg gcaagcatgc cgggctgaaa acggcgtaaa cggaagcgcg gctagtgaag
     2101 aaacaagatg gacaaatcct attaccctct cggttcggtc catcttcttt gattcgggtt
     2161 agttcaagtt tgctgctgga cttgtgaaga aacgcacgca ttagaacaag ctgagctgat
     2221 caacgctggt cctgcgaaaa tgttccacag aaaattgcgg ccaacgatct cggatgcctg
     2281 cttttttctg tgcaagctga aacagctcgg ccatctgatc ggcgaaaatg ccctcgccgc
     2341 gcatgcgact tttaaaattt ggatcattga gcttgccggc tcgcattgag cgaatccgat
     2401 ttaaaacctt ttctttcttg ccaggatagt gctgctccag ccattgctcg aacagtaact
     2461 tgaccgcaaa aggcagacgc agcgccacat aaccgcaaaa tttcgcgccg gctttggccg
     2521 cggcctgagc gatcgcgggt gcctctgaat cggtgagagc tggaatcatc ggcgcttgaa
     2581 gataacccac cggcacacca gcttcggata acgtccggat tgcggcaagc cggcgctcgg
     2641 gagttgatgc ccggggctcc atcaaagatg ccagcttgtt gtccaacgtc gtaatggaaa
     2701 tagtgacgcc aatgcattgg tatttcgcca attctgatag aagatcgata tcgcgcgtga
     2761 ccaggtggtt ctttgtaata atgactaccg gattgcgaaa ttccaggaat acttccaagc
     2821 agcgcctcgt tagctgtttc ttcttctcga ccggctgata acagtccgta ttgccgctta
     2881 atgcaatgat ctgaggcttc catttatggc ttgaaaattc actgcgcaat aactccggcg
     2941 cgtcttcttt caccatgatt ttggtttcga aatccagccc ggaagaaaaa ccaaggtact
     3001 cgtgtgtggg acgagcatag caataaacgc atccatgctc gcaaccccga tacggattga
     3061 cgctggcctt gaagccaacg tccgggctgt cgttgtaggc aactagtgat ctggaatcat
     3121 ccgcgaaaaa ttgtgtttga gctgaggaga tttcatcgca gtagtccgac aattctggaa
     3181 tacgctcaat cgattcgaag cggttctttg ggttggtgat cgatcccctg ccgcggaccg
     3241 gggacggcga cttcatgccc ttattccccc gctgcggctg cgccggtctt ctcagccgag
     3301 cccttatttg ctgattctgc cgccaatttt ttcttctgct tcgccaaatc gcgcgtttgg
     3361 cgcttgagca gcttgatttt cctgcgaatg cgcttcacct ttgagctatc gccggaagaa
     3421 gttaggattt cagccttctc tttcttcagt tgccgaatac ttagcttgat attgggcaat
     3481 gcgctcacat ccattttatt atttcctcca ttgcagttac tcctgctaac atatttctct
     3541 gggaaaccaa agcgcgacta cggtatcaag tcacggccga tgacacggca agataagagt
     3601 cgaacggatc attgagcggc tggagaagat ccatgccgat gctaaactca attttggaat
     3661 tgctgatgcc ttaattttga cagcgcaagc aagagatgag ttagttcgac aaatcactcc
     3721 tgatctgttg gcaaaatacc gaagcgccga ggattacgcg aaaacgcagg acatcggtaa
     3781 gaatcactcg aaacaactcc cgatctattc gtaactgctg tactgaattc atcaaagggt
     3841 tcgctgaggc gagtaccaga caaattggat ggtctacttt cgctccgcgg ttgggacgga
     3901 aaacggccaa tatgttactg cgtaatgcct tcggtaaaga caatcggcgg cgttcgacac
     3961 tgcccgtgcc tcatcgacgc atccatttga aatatccaag aaaaaaaagc cccaacttcg
     4021 aacgaagttg aggctttgaa attaatccgg cagcgtccta ctcttccata gagcgactct
     4081 atagtaccat cggcgctgga gggcttaacg gccgagttcg ggatgggatc gggtgtgaca
     4141 cctccgctat cgccaccgga aacttataaa cggtgaacct ttgagggtta aacggatgtt
     4201 ctttcacgac tgaatacggg caacggaaac ttacgcggag tcatattggt aaatatggtc
     4261 aagccgcacg accaattagt accggttagc ttcaatcatt actgatcttc cacatccggc
     4321 ctatcaacct cctagtctag aaggggtctt tagaggccta atggcctggg ataccttatc
     4381 tcggaggggg cttcccgctt atatgctttc agcggttatc ccgtccacac atagctaccc
     4441 ggcaatgcgg ctggcgccac aaccggaaca ccagcggtgt gttcttcccg gtcctctcgt
     4501 actagggaaa actcccctca agtatcctgc gcccacggcg gatagggacc aaactgtctc
     4561 acgacgtttt gaacccagct cgcgtgccac tttaatcggc gaacagccga acccttggga
     4621 cctacttcag ccccaggatg tgacgagccg acatcgaggt gccaaacacc cccgtcgatg
     4681 tgaactcttg gggggtatca gcctgttatc cccggcgtac cttttatccg ttgagcgatg
     4741 gcccttccat gcgggaccac cggatcacta tgacctgctt tcgcacctgc tcgagatgtc
     4801 tctctcgcag tcaagccggc ttatgccatt gcactcgatg gctggtttcc actcagcctg
     4861 agccgacctt cgcgcgcctc cgttacaatt taggaggcga ccgccccagt caaactaccc
     4921 accatgcaat gtccctcagc tggatgacag ctgtaggtta gaatccaagg ccagtaaggg
     4981 tggtatttca aggttggctc cacgagagct agcgcccccg cttcacagcc tcccacctat
     5041 cctacacata ctaaccctag atccattgca aagttatagt aaaggtgcac agggtctttc
     5101 cgtcctgccg cgggtacccg gtatcttcac cgagagtgca atttcgctga gtccctggtc
     5161 gagacagtgg ggaaattgtt acgccattcg tgcaggtcgg aacttacccg acaaggaatt
     5221 tcgctacctt aggaccgtta tagttacggc cgccgtttac cggggcttcg gtt
//
LOCUS       DASUTG010000484         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_223332, whole genome shotgun sequence.
ACCESSION   DASUTG010000484 DASUTG010000000
VERSION     DASUTG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223332"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VF089_11060"
     CDS             complement(<1..463)
                     /locus_tag="VF089_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174544.1"
                     /translation="MNYRTIDDMLEPGTLVQLTDLWTPALARYRDRPAVVIDIEVLGY
                     PRSISRQENGTSVSEKYDCIYTVALMPAERTLTLAREQVPVIRVISLDVVGILAADPE
                     SANFAVWCAREFCGAEPASIPQPIVTGNGRVIGGAVLPHTIPMYEARHRPED"
     gene            766..1605
                     /locus_tag="VF089_11065"
     CDS             766..1605
                     /locus_tag="VF089_11065"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174545.1"
                     /translation="MPDLAQAPSRSARSQASSVIKVEDVSKFFQTPSQENLGALLNID
                     LEIRHGEFVTIVGPSGCGKSTLLKLIAGFSPVSGGRIMFQDDEVRGLNTRVGYVPQES
                     KLFPWLTVEENVGFGLSPKKYSRADKERQVSYFIKLAGLSGFEKYYPAQLSGGMGKRA
                     SIIRALAYEPPVILMDEPFGPLDAQTRMVLQDELLKIWEQKRQTIVFVTHDLVEAVAL
                     ADRVVVMTHRPGKIKDVIKINMARPRNIFEIHRQEGFDDAYGRLWNIFRHELNVGRPE
                     QPT"
     gene            1602..2459
                     /locus_tag="VF089_11070"
     CDS             1602..2459
                     /locus_tag="VF089_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174546.1"
                     /translation="MTEQIGQDRSRLAPSELRTTPAKPIFSKRVLTVTLIRTAIVLGF
                     LILWELASGRWIEPFLISSPSRILTSLLTGFSSGELLQHTWVTFLEVAIGFPIGAITG
                     IALGYGFGRSRLLAEVFEPIIIALNGIPRTALAPLFIVWLGIGLWSKVGVVFLLTFFL
                     NFFNTYTGMRQMDQEYVDLAGLMGVRGWKLTLKVIFPAISPYVFTGIRTSIPFAVIGA
                     IVGEFVAATEGVGFFIRMSAGIFRTADVFVGIIVLMIMVIIMDKIAEAVERRALRWQT
                     QKEHVQIQG"
BASE COUNT          564 a          690 c          700 g          623 t
ORIGIN      
        1 gatcttcggg gcggtggcgc gcctcgtaca tgggaattgt gtgcggcagc acggccccgc
       61 cgatgactcg gccgttgccg gtaacgatcg gctggggaat cgatgctggc tccgcgccgc
      121 agaattctct cgcgcaccag acggcaaagt ttgccgactc cggatccgct gcgagtatcc
      181 caaccacgtc caaagatatc acccgaatca cgggaacctg ttcccgcgca agggttagcg
      241 tgcgttcggc cggcatcaat gcgacagtgt agatacaatc gtacttttca ctgacggacg
      301 tgccgttttc ctggcgactg atcgaacgcg ggtagcccag cacttcgata tcgatgacga
      361 cggcgggtcg atcgcggtaa cgggccagcg ccggcgtcca taaatcggtc agttgtacca
      421 aagtgcctgg ctccaacatg tcatcgatgg ttcggtaatt cattattcac cccgcggaat
      481 gttgacgctg atagcttcgc caacttagca gaatattaca gcggtaagct acaatctaaa
      541 gatggcggga tcgtctggca tcttattaag ttttttgagc gtcagcctgc aacgttcaaa
      601 catccgccgc gctcgccgtg taatcttcaa ccgctcgatt tcgtgtcact gcgtttggac
      661 ggcccggcga atcgctgttc tttaatgggc cgtaccaaag tcctaatacg gttgacacac
      721 taagggttag atggataaat accgcgcaag gtaagcacga agctaatgcc agacctcgca
      781 caggctcctt cccgatcggc gcgcagccaa gccagcagcg taatcaaggt tgaggacgtc
      841 tcaaaatttt ttcaaacacc gagccaagaa aacctaggcg cgttgctaaa cattgatttg
      901 gagatccggc acggcgagtt tgttactatc gtcggcccca gcggctgcgg caagtccact
      961 ttgctcaagc taatcgctgg cttttcaccg gtatctggcg gccgcattat gtttcaagac
     1021 gatgaggtgc gcggtctcaa caccagagtc ggttacgtcc ctcaggagag taagctcttc
     1081 ccctggctca cagtcgagga gaacgttggg ttcggattga gcccaaagaa gtactcccgg
     1141 gccgataaag agcggcaagt gagctatttt atcaagctcg cgggcctttc cggttttgaa
     1201 aaatattatc cggcgcagct atcgggcggc atgggtaaac gggcttcgat tatacgcgcg
     1261 cttgcttatg aaccgccggt gatcttgatg gacgagccct tcggtccatt agacgcgcag
     1321 actcgtatgg tgctgcagga tgaattgttg aaaatctggg agcagaagcg acaaaccata
     1381 gtttttgtca ctcacgattt ggttgaagcc gttgctctag cggaccgcgt cgtcgtcatg
     1441 acgcatcgtc ccggaaaaat caaagacgtc atcaaaatca atatggcgag gccgcgcaac
     1501 atttttgaaa ttcatcgcca ggaaggtttt gacgatgcct acggccgttt atggaacatc
     1561 ttccgccacg agctgaatgt cggccgccct gagcaaccaa catgacagaa cagatcggtc
     1621 aagatcgctc tcggctagcg ccttcggagc tccgcaccac accggcaaag ccgatattct
     1681 ctaaacgggt tctcaccgta acgttgattc gcaccgctat cgtcctcggc tttcttatat
     1741 tgtgggaact cgcctctggc agatggattg aaccgtttct catcagcagc cctagccgta
     1801 ttcttacgtc gctcttaacc ggtttctcct cgggtgaatt gttgcagcac acctgggtga
     1861 cttttctaga agtcgccatc ggctttccca tcggcgctat cacgggtatc gcgctcggct
     1921 acggtttcgg ccgtagccgc ctactcgccg aggtttttga accgattatc atcgcgctca
     1981 acggcatccc acgcaccgcg ctggcgccgc tttttatagt ctggctgggc atcggccttt
     2041 ggtcgaaagt cggcgtggta tttctcctta cgttcttttt gaattttttt aacacctaca
     2101 ccggcatgcg gcagatggat caggagtatg tcgatttggc tggcctgatg ggagtccgcg
     2161 gctggaagtt gacgttaaaa gtaatcttcc cggctatttc tccgtatgtt tttaccggta
     2221 ttcgcacaag cattcctttc gcagtgatcg gcgccatcgt cggggaattc gtcgctgcta
     2281 ctgaaggggt gggctttttc atccgcatgt cggccggcat tttcagaacc gccgacgtgt
     2341 tcgtcggcat tatcgtgctg atgatcatgg tgatcataat ggataagata gccgaggctg
     2401 tggagcgccg ggcattacgt tggcagacgc aaaaagagca tgttcagatc caggggtgag
     2461 aatgaaagga gaacgagcaa tgaagaggct catgaaaatc gcgcgcttga tgatcgcgct
     2521 ctctgcttgc gtgcttgttg gcacaaccgc aaagattcac gcgcagtcca aggaaat
//
LOCUS       DASUTG010000485         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_518787, whole genome shotgun sequence.
ACCESSION   DASUTG010000485 DASUTG010000000
VERSION     DASUTG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518787"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VF089_11075"
     CDS             complement(<1..954)
                     /locus_tag="VF089_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6174547.1"
                     /translation="MSNKISRRHMLGLGAATIAGVALSQSPAQAINHGGSGADYKPFR
                     GYDPRRGGDRPYWEKSYSGGAVDVKPLPPVLPGKGYKPVVVPDGAALPFKIVDGVKVF
                     HLVAEEVDHYFDSGLRAKCWGYNGRVNSTVIEAVEGERVRIYVTNRLTVATSVHWHGF
                     NLPNGMDGVGGLTQPYIKAGETVKYEFTLRQYGTQMFHSHHDEMTQMGMGLIGMFIVH
                     PRNPSSEYHVDRDFSLMISEWAIPAGTSRPNTLEMTDFNVLTINGKVFPSTSPLVCKT
                     GDKVRIRLGNLGATDHHPMHIHGHHFRVTATDGEEIPLSAQW"
     gene            complement(973..2394)
                     /locus_tag="VF089_11080"
     CDS             complement(973..2394)
                     /locus_tag="VF089_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6174548.1"
                     /translation="MGWRENIGWLSIGLLFFSGCTSMTLDAGFDDIKATVERRSSLQI
                     SWNNGTELDQEASEKLNSLLKRKLTADDAVQIALLNNRDLQAVYSDLGVAQADLVQAG
                     LLGNPIFDAAVMFPVSGGGRPDLEFGAAMNFLNIFYIPLRKRVAAARFEEVKTRVAGS
                     VVDFAGRVRTAFYTHQSDQQMLELRQTIVQALSASLEIARRLSEAGNITDLDFTRERA
                     QLETAKLAVRSAETALRQSREELNIVMGLWGEQTQWQSDGRLSDIPEQPVPMKDIERL
                     ALEQSLDLANARQRIAATSEQLGLTRWTTLLPELSTGPQAERNDGEWEIGPRLEFWIP
                     LFDQGQARVGRGVAELRRARQEYYGAAVRIRATARALRDRVDGARERALYHRDILLPL
                     QERIVNEAQLQYNAMQLGPFQLLRAREQQIQTAVAYIESLRDYWLARADVEQILSGRL
                     PDSRRGSNRTASGQSETIDSEGH"
     gene            complement(2426..2881)
                     /locus_tag="VF089_11085"
     CDS             complement(2426..2881)
                     /locus_tag="VF089_11085"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX6174549.1"
                     /translation="MKSVALSLATVFLALSACAAYESQPVGINHPANPRAMSAPEGAP
                     SKTLAYTRNDMQAIAAAAQQERQEGQDAAGAANGQKTVVGEGKVIATVPNANQLVVEH
                     GEIKGFMEPMTMGYRIEPASLLEGLQSGDQIRFTIDVSKKTIVKVEKMK"
     gene            complement(2962..3504)
                     /locus_tag="VF089_11090"
     CDS             complement(2962..3504)
                     /locus_tag="VF089_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6174550.1"
                     /translation="MEKLTSLLKNVDEFTGFARKRLADPELAADAVQESLLKALKAAD
                     QIRDEENAKAWFYRILRRTIIDLYRRRDARDRAVAALERELNSPADQEEARVVCACME
                     RLLPIMTPQYSVLLQRIDLNEESPDSVAASLGISRSNLNVRLHRARQQLRRRLEENCR
                     VCAKHGCFDCHCGTDAKRPA"
     gene            complement(3509..>3954)
                     /locus_tag="VF089_11095"
     CDS             complement(3509..>3954)
                     /locus_tag="VF089_11095"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX6174551.1"
                     /translation="TEHAPELSQMGAMHPVEFFAESIMDPNAVVPKIYREANGKSPMT
                     NFAEKMTVQELIDVSAYVASLRPKNAPKSVTAQGQVVALVPESSEIVLTHGEITGFME
                     AMTMGYKVSSPSLLKSVKPGDTVQFTVDTDKRVITKIAKAQGQKR"
BASE COUNT          821 a         1170 c         1023 g          940 t
ORIGIN      
        1 ccactgcgcc gacagcggaa tctcctcgcc gtccgttgcg gtcacccgaa aatggtgtcc
       61 gtgaatgtgc atcgggtggt ggtcggtcgc gcccagattg cctagacgaa ttctaacctt
      121 gtcgccggtc ttgcacacca gaggcgatgt cgatggaaac actttgccgt tgatcgtaag
      181 aacgttgaag tcggtcattt cgagcgtatt cggacgagag gtgccggccg ggatggccca
      241 ttcactgatc atcagcgaga agtcgcgatc gacgtggtat tcagacgacg gatttctcgg
      301 atgtacgata aacatcccga tcaaacccat acccatctgc gtcatctcat catggtgcga
      361 gtggaacatt tgcgttccat actgccgcaa agtgaattcg tacttcaccg tttcaccggc
      421 tttgatatac ggctgagtga gcccgccaac accatccatg ccgtttggga gattgaagcc
      481 atgccagtgc accgaagtgg cgacggtcaa acggttcgta acataaatac gcacgcgctc
      541 gccttcgacg gcttcgatga cagtgctgtt tacccggcca ttgtatcccc agcacttggc
      601 gcgcaatccg gagtcgaagt aatggtcaac ttcttccgcg accagatgaa aaactttcac
      661 tccatcgacg atcttgaaag gcagcgctgc gccgtcggga acgacgacgg gtttgtatcc
      721 tttgcccgga agaaccggcg gcaacggttt aacgtcgacg gctccaccgc tatagctctt
      781 ttcccagtag ggccgatcac ctccccggcg ggggtcgtat cctctgaatg gcttgtaatc
      841 tgctccagag ccaccgtgat tgatcgcctg cgccgggctt tgcgataaag ccacgccagc
      901 gattgtggcg gcgccgagac caagcatgtg acgccgggat attttgttac tcatctcgta
      961 ctcctgaaaa tattaatgtc cttcactatc gatagtctcg ctttgcccgc tcgcagttct
     1021 gttcgatccg cgtctcgagt cgggtagccg accgctcagg atttgctcca catcagcgcg
     1081 ggccaaccag tagtcacgaa gcgactcgat gtaagcaact gcggtttgta tttgctgctc
     1141 gcgcgcgcgc aagagctgaa acggacccag ctgcatggcg ttgtattgaa gctgcgcctc
     1201 gttgacaatg cgctcttgca gcggcaacag aatatcgcga tggtacagtg cgcgttcacg
     1261 tgccccgtct actcgatcgc gtagggctcg cgcggtcgca cggatccgga ctgccgcgcc
     1321 gtagtattct tgccgggctc gacgtagttc cgcgacaccg cggccgaccc gcgcctgacc
     1381 ctgatcgaac aagggaatcc aaaactcgag tctggggccg atctcccatt ccccatcatt
     1441 acgctccgcc tgaggaccgg tgctcaattc cggaagcaag gttgtccacc tggtcaatcc
     1501 aagctgttcg cttgtggctg caatccgctg cctggcattt gcaaggtcca ggctctgttc
     1561 cagcgccagc cgttcaatat ctttcatcgg cactggctgc tccggaatat cagacaaacg
     1621 tccgtcgctt tgccattgtg tttgttctcc ccacaatccc atcactatgt tcagttcttc
     1681 tcggctttgc cgcagcgcag tttcggccga tctaacagcc aacttggcgg tctccaactg
     1741 tgcccgttcc cttgtaaaat cgagatccgt aatgtttccg gcttcagaaa ggcgacgtgc
     1801 gatttccagc gaagcactca gcgcctgaac aatcgtctgg cgcagctcga gcatttgctg
     1861 atccgactgg tgtgtgtaaa acgcggtccg cacacgcccg gcaaaatcaa caacggaacc
     1921 ggctacccgg gttttgacct cctcgaatcg tgccgccgca actcgcttcc gtagcggtat
     1981 ataaaaaata tttaaaaagt tcatcgcagc gccgaattcg agatcgggcc gaccgccgcc
     2041 ggaaaccggg aacataaccg cggcatcgaa gattggattg cctaaaagtc ccgcttgcac
     2101 cagatccgct tgcgcgacgc cgaggtcgga gtataccgct tgcaggtcgc gattgttgag
     2161 caaggcaatc tgaacggcat catcggcagt caacttgcgt ttcagcaaag agtttagttt
     2221 ctctgatgct tcttgatcga gctcggtccc attgttccaa gaaatctgga gggagctccg
     2281 tcgttcgact gtggccttga tatcatcgaa accagcgtcg agcgtcatcg atgtgcagcc
     2341 gctgaagaat aaaagaccga tgctcaacca tccgatattt tcccgccatc ccatcaggcg
     2401 atctcccttt tggacttggg taatctcact tcatcttttc aaccttgacg atcgtcttct
     2461 ttgaaacatc aatcgtgaag cgaatctggt ctccggattg aagtccttcg aggagtgagg
     2521 ccggctcgat ccgatatccc atcgtcatcg gctccatgaa gcctttgatt tcgccatgct
     2581 caactactag ttgattcgca ttaggaacgg ttgcgataac cttgccctct ccaacgacag
     2641 tcttctgccc gttagctgca ccagccgcat cttgaccctc ctgcctctct tgttgggccg
     2701 cggcagctat cgcctgcatg tcgtttcggg tgtaggcaag tgttttggaa ggcgcacctt
     2761 ccggcgcgct catcgcccgc gggttggccg gatggtttat gcctaccggc tgtgactcat
     2821 aagccgcgca agctgacaac gccagaaaaa ccgttgcaag cgaaagagct actgatttca
     2881 tctgtttttc tccttagggg tttggctaac caagctgacg catgagccgg gctttcatta
     2941 cacgctgacg ccgagaaact tttaggcagg cctttttgcg tcggtgccgc aatggcaatc
     3001 gaaacaaccg tgctttgcgc aaacccgaca gttttcttcg agacggcgcc tcagctgctg
     3061 ccgcgcccga tgaagccgca cgttaagatt gctccggcta attccgaggc tcgcggccac
     3121 agaatcgggc gattcttcat taagatctat gcgctgcagc aagacagaat actgcggcgt
     3181 cattatcggc agcagtcgct ccatgcacgc gcaaacaacc cgcgcttcct cttgatctgc
     3241 cggtgaattt agctcgcgct cgagtgctgc aacggctcga tcccgcgcgt cacggcggcg
     3301 gtaaagatcg ataatcgtcc gccgcaggat ccggtagaac cacgccttgg cattttcctc
     3361 atcccgaatc tgatcggccg ctttcagcgc cttgagcaag ctctcttgaa ccgcatcggc
     3421 ggcaagttcc ggatctgcaa gacgcttgcg cgcaaagccc gtgaattcat caacgttctt
     3481 cagcaaggaa gttagcttct ccataagctc accgcttttg accttgagct ttggcgatct
     3541 tcgtaattac ccgtttgtcc gtgtcgacag tgaattgaac ggtatccccg ggcttcacgg
     3601 atttgagcag cgaaggcgag ctgactttgt aacccatggt catcgcctcc atgaatcctg
     3661 tgatttcacc gtgcgtcagg acgatctcac tgctctccgg caccagagcg acgacttggc
     3721 cttgcgcggt cacgcttttc ggcgcgtttt ttggcctgag cgaagccaca taagccgaga
     3781 catcgatcaa ttcttgcact gtcatcttct cggcaaagtt ggtcatcgga gacttaccgt
     3841 tggcctcgcg atagatcttc ggaacaaccg cattcggatc catgatggac tccgcaaaga
     3901 actccacggg atgcattgcc cccatctggc tcaactcggg cgcatgttca gtag
//
LOCUS       DASUTG010000486         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1705650, whole genome shotgun sequence.
ACCESSION   DASUTG010000486 DASUTG010000000
VERSION     DASUTG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705650"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF089_11100"
     CDS             complement(<1..717)
                     /locus_tag="VF089_11100"
                     /inference="COORDINATES: protein motif:HMM:NF042862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-like protease helical domain-containing
                     protein"
                     /protein_id="HEX6174552.1"
                     /translation="MARKFEVPPDKLSWRCDLSYLPFTCTAEMTPLVDFIGQDRAIRA
                     IQFGLGVNKPGFNIFVAGLTGTGKTSIIKAFLKKATTERAPVEADAESPADWCYVYNF
                     TDPDRPQIIKMRRGWGKVLKFDMEQLIQYVQREGRRLFEGDEFARDRQSLVEELQRKQ
                     QGLMEPLIAEAGQNGFTLRMTPSGVALLPIKEGKPMQEADYLALPPEQKKALEDKRSE
                     LGKRIEEILREGKKVEREINR"
     gene            886..2253
                     /locus_tag="VF089_11105"
     CDS             886..2253
                     /locus_tag="VF089_11105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6174553.1"
                     /translation="MLLTCLFITACASYRSPLPSVSGPSEDEETRISREFRREAKKHF
                     TYVNNPEIERYIERIGQRILSAMGPQPFDYRFFVVADPQLNAFAVPGGSVFFYSGLIE
                     RAKSTAEIAGVMGHEVVHIKGRHMARHSGPDAISLLSLVGMLLLARSGSGAQAAGVVG
                     QAVSATRQIAYSRQLEMEADTLGVRYMTAAGYDPAGSLGFLKTLDQERSLNPIDLPAY
                     MMTHPVTQERVANVQLVIRSLGDVQTKSERLDQLKKIQVLIRLARQETDKVIDEFQKL
                     VAQAPSDSVALHLLALAQHHKGQLPQAKANYEKALKLSSDDPGLYRDLGRLYTDLGDF
                     SAGRAMLDQALTMEPKEPLNYLYLGELLEKQNDLRAAAGAYLNAHQLSPLWDKPPARL
                     GIIYGNLDRLGDAYYYRARSLLLQDEDERAIADLEKAIKILGENSARGQTVKDELNNL
                     RARRR"
     gene            complement(2233..2631)
                     /locus_tag="VF089_11110"
     CDS             complement(2233..2631)
                     /locus_tag="VF089_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174554.1"
                     /translation="MYDDFLARIVEPDVFLPSQFYGSGGLSRQLDGEKRLMIAILKDA
                     VECLNKYRGARSSLGSSQYQNALEWIEDEGTEWLFSFNNICDLLGFDPDYLRKVLIKK
                     EGRYASSARGKIYSFPVANRAGRGSAPASS"
     gene            complement(2920..3630)
                     /locus_tag="VF089_11115"
     CDS             complement(2920..3630)
                     /locus_tag="VF089_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6174555.1"
                     /translation="MSDLSEIKKKLAMSSRMLFNAGLVDYSGHISARIPGSDHLVILP
                     HPVSRATVKPDDMVITDFDGKLIEGKYKAPSEVNMHARAYKARSDIQSVAHLHNHMVA
                     TLSMVDKPFYPASSNPGAFFGPGPLPVYMDPALIHTIEQGDAVAKALGTADAVMLRGH
                     GSMVVAHSIEWVFAGCVDLEEAAARFYKASLLGPVTIYNEDETQRVMKQRRKDSVVQK
                     IWDHNAAKTELAGLMKGI"
     gene            complement(3929..5383)
                     /locus_tag="VF089_11120"
     CDS             complement(3929..5383)
                     /locus_tag="VF089_11120"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HEX6174556.1"
                     /translation="MKTLIQGGFVIGFDGTQHEIIKDGSVVYDGNRIEFVGKDYSQAI
                     DTKIDAKGCLVSPGFIDTHFHSGINASDYILNDSTKLDFFAANYLAHGGPTREGAHSA
                     HLKDVDVGQRFSLIHLLKSGVTTTFEIGGPGAEPERFVDMVDEVGIRTATGPSYKNVS
                     FFHDHAGRLEYDWDEARGSEGLRKAVQFAKQFDGACNGRMSAMLFPGHVDSCTPDLLK
                     QTKKAAHDAGVRIQIHATINLFEFHTVMQRERCTPIELLQRIGFLDPQVSLSHCIFIS
                     GHSWLAYPHGNDLKIIADSGASVAYSPLKYLKLGITMESFDRYLNAGINMGIGTDTFP
                     KDILSDMRYAALASRVADKSFMSGHPRDVFNAATLGGAQMLGRNDLGRLEKGAKADIA
                     IVNLRDLTFGAVRDPIRSLVENAVSRDVRTVIVDGETLVDKYKYLRLDEEELLDKVQA
                     KGEEVWDSVPKWHWTGKTIDEVVPPSFKTKQSLS"
BASE COUNT         1344 a         1549 c         1461 g         1374 t
ORIGIN      
        1 tcggttgatt tcgcgctcta ctttcttccc ttcacgcaaa atctcttcga tcctcttgcc
       61 gagttcgctg cgcttatcct ccagcgcctt cttctgttca ggaggcaatg cgaggtaatc
      121 ggcctcctgc atcggtttgc cttctttgat cggcagcagc gcgactccgg atggagtcat
      181 ccgtaacgtg aagccgttct ggccggcctc ggcaattaac ggctccatca ggccttgttg
      241 ctttctctgc agctcctcca cgagtgactg gcgatcgcgt gcgaattcgt ccccttcaaa
      301 caatcttctg ccttcccgtt gcacatattg aatcagctgt tccatgtcga atttcaggac
      361 cttaccccag cctcgacgca tcttgataat ctgcgggcga tccggatcgg taaagttgta
      421 aacatagcac cagtccgcag gcgattcggc atccgcctca accggagcgc gctccgtcgt
      481 tgccttcttg agaaaagctt tgatgatgct ggttttgccg gttccggtta gcccggcaac
      541 aaaaatattg aagcccggtt tattgacgcc gaggccgaac tggatcgcgc gaatcgcccg
      601 atcctgaccg ataaaatcca cgagcggagt catctccgcc gtgcacgtga agggtagata
      661 cgataggtcg caacgccagg acagcttgtc tggcggaact tcgaatttac gcgccatgaa
      721 aatacctcga gctgtgggtc gagtcagaat atggcgtcca tgagaaagcg tcaactgctc
      781 accacttggt ggagaaacgc ggttgaaccg gctccgacgc ctcactataa tctttgagtg
      841 attcaaacct acaggtcccg tgcttgccag aagctgtggt cgtggttgtt gctgacttgc
      901 ctcttcatca cagcttgcgc ttcttaccgg tcgccgctgc cgagtgtcag cggccccagt
      961 gaagacgagg aaacacggat cagccgcgaa tttcgccgcg aggcaaaaaa acactttacc
     1021 tacgtaaaca atccggagat cgaacgatat atcgaacgca tcggccaacg gattctgtcg
     1081 gcgatgggtc cgcaaccttt tgactatcgc ttctttgtcg tcgctgaccc gcagctaaac
     1141 gcgtttgcag tgcccggagg ttcggtcttc ttttactccg gccttatcga acgggcgaaa
     1201 agcactgctg aaatcgccgg agttatggga cacgaggtag ttcacatcaa agggcgtcat
     1261 atggcgcgcc actccggtcc ggatgcgatt tcgctgttaa gtttggtggg gatgcttctg
     1321 ttggctcgta gcggttcggg cgcgcaggca gcaggcgttg ttggccaagc ggtttctgct
     1381 actcgtcaga tagcttatag ccggcaattg gagatggaag ccgatactct aggggtccgt
     1441 tacatgacag cggcgggcta tgaccctgcc ggttcgcttg gatttctcaa gaccttggat
     1501 caggaacgat ctttaaatcc gatagacctg cccgcctata tgatgactca tccggtcacc
     1561 caggaacgag tcgccaacgt tcaactagtc attcgctccc tcggcgatgt tcaaaccaag
     1621 tcggaaaggc ttgatcagct taagaaaatt caagtactca ttcgtttggc gcggcaggag
     1681 accgacaagg tgatcgacga gtttcaaaaa ctggtcgcgc aagctccgtc ggatagcgtc
     1741 gcgcttcatc ttttagcgtt ggcgcaacat cacaaaggcc agttgccaca agcaaaagcc
     1801 aactatgaaa aggcgctgaa acttagctcg gacgatcccg gtttgtaccg tgacctcggc
     1861 cggctataca ccgacctcgg cgatttcagc gccggccggg ctatgcttga tcaggcctta
     1921 accatggaac ccaaggaacc gctcaactat ttatatctcg gagaattact ggagaaacag
     1981 aacgaccttc gcgccgcggc cggcgcctat ctcaatgccc accagctgtc gccactatgg
     2041 gataaaccgc ctgcccggct tgggatcatc tatggaaatc tcgaccggct tggagacgcc
     2101 tactactaca gggcccggtc tttattgttg caggacgaag acgagcgcgc tatagccgat
     2161 cttgaaaagg cgatcaaaat actcggcgaa aactctgcac gcgggcaaac agtaaaagac
     2221 gaactgaata atttacgagc tcgcaggcgc tgagcctcta cccgctctat tagcgaccgg
     2281 aaaagaatag atcttgcctc gggcagaact cgcgtatctg ccttcttttt tgatcagcac
     2341 cttgcgtaga tagtcgggat caaatcctaa aagatcgcag atattattaa acgaaaacag
     2401 ccactcggtg ccttcatcct cgatccattc cagggcattc tgatactgac tacttcctaa
     2461 actacttcgc gcgccgcgat acttatttag acactctacc gcatccttca aaatagcgat
     2521 catcaatcgt ttctcaccat cgagctgacg cgacagccct ccgctgccgt agaactgaga
     2581 cggcaaaaaa acatcaggct caacgatcct tgctagaaaa tcatcgtaca tatgcggaac
     2641 actcccgggt gaaattgaat cgcaagctat atcacgggag tttcttccac gcaacaaatt
     2701 tcatcatacc agcaatgaaa ccgcacgagc tgcaatgcgc gcgcggggca cattagcggc
     2761 aaaacaggag atgttctggg caaaaacttg agcttcattc gcccggaatg agcgcagtaa
     2821 acaatgccga atgaacccta tacgaattga acaggcgcgt ccgggctccg gcgctcccat
     2881 gttcacctct gcgcttcgat ccgcgacggg tgctgtcgtt tagatccctt tcatcagacc
     2941 ggcaagttcc gtcttagcag cattgtgatc ccagatcttc tgaaccaccg aatctttgcg
     3001 gcgctgtttc atgacccttt gtgtctcgtc ctcattgtaa atcgtgacgg gccccagcaa
     3061 tgaagcttta tagaaccggg ccgcggcttc ttccaaatct acacatccgg cgaataccca
     3121 ttcgatagag tgggcgacca ccatcgagcc atggccgcgc agcatgacgg catccgccgt
     3181 gccaagcgct tttgccactg cgtcgccctg ctcgatcgtg tgaatcagcg cggggtccat
     3241 gtaaacaggt aatggtcccg ggccaaaaaa agcgccggga ttggaggaag ctggatagaa
     3301 cggtttgtcg accatgctca acgtggcaac catatgattg tgcaaatgag cgacactttg
     3361 gatatcacta cgggctttat aggcccgagc gtgcatatta acctctgaag gcgccttgta
     3421 cttgccctcg atcaatttgc catcgaagtc ggtgatcacc atatcgtccg gctttaccgt
     3481 agcgcgactc accggatgtg gcagaataac aaggtgatca gacccaggaa tccgcgcact
     3541 gatatgaccg ctgtagtcga ctaaaccggc gttgaacaac atgcgggagg acatagccag
     3601 tttttttttg atctctgata agtccgacac gatttactcc tctcagttga acttgttgtc
     3661 tgtgctatat ctttcctcgg atgatagttc aaccgaaaga cgaccctgga gcgcaaaacc
     3721 cggagcaaaa cgagcagcaa ctgcgatgat ttaaaatcca ctaagttgca tcggctcaaa
     3781 gaagccgatg cagaggaaac gattgaggag attgcggttt gccgctaaac tgcggcgcgt
     3841 agatgtctaa ccgaaggtct agtgctttaa gccgtcagct cctgcggaat gccaggctag
     3901 caatctggtt tgaccctagg ccgggcgttt agcttaatga ttgcttcgtt ttaaagcttg
     3961 gcggcactac ttcgtcgatg gtctttccgg tccaatgcca tttggggaca ctgtcccaga
     4021 cctcttcccc ttttgcctga actttgtcga gcagctcttc ctcatccaga cgaaggtatt
     4081 tgtatttatc gacaagtgtt tcgccgtcaa cgatgacggt tctcacgtcg cggctgacag
     4141 cgttctccac caatgatcga atcggatcgc gcactgcgcc aaaggtgagg tcgcggagat
     4201 tgacaatagc gatgtccgct tttgcccctt tttccagtct tccgagatcg ttgcgcccga
     4261 gcatctgggc cccgcctaaa gtcgctgcat tgaagacgtc tcgcgggtgg ccggacatga
     4321 agctcttatc cgcaacacgc gaggcgagcg ccgcgtaacg catgtccgac agaatatctt
     4381 tggggaaagt gtcggtacca attcccatgt tgattccagc attgagatac cggtcgaagg
     4441 attccatggt gatacctagc ttgaggtatt tgagcgggct gtaggcgacc gaagcgcccg
     4501 aatcggcgat aattttaaga tcgttgccgt gtggataggc aagccagctg tgaccgctga
     4561 tgaaaatgca atgggaaagc gaaacctgcg gatccaggaa gccgatcctt tgcagcaact
     4621 cgataggcgt gcagcgttca cgctgcatga ccgtatgaaa ttcaaacaga ttgatcgtcg
     4681 catggatctg aattcgaaca ccggcatcgt gtgccgcttt cttggtttgt ttgagcagat
     4741 cgggtgtgca ggagtcgacg tgcccgggaa acaacatagc gctcatgcgc ccgttgcaag
     4801 cgccatcgaa ttgctttgcg aactggaccg cttttcttaa gccttcgctg ccccgagcct
     4861 catcccagtc gtattctagg cggcccgcat ggtcgtgaaa aaaactgacg tttttatagc
     4921 tcgggcctgt cgccgttcgg atgccgacct cgtcaaccat gtcgacgaac cgctccggct
     4981 ctgcgcccgg accgccgatc tcgaaagtgg tcgtcactcc gctttttaat agatggatca
     5041 gagaaaaccg ttggccaaca tcgacatctt ttaagtgagc gctatgagcg ccttcacggg
     5101 tcggcccgcc atgggctaga taattggccg cgaagaaatc caatttcgtg gaatcattca
     5161 ggatgtagtc gctggcattg atccctgaat ggaaatgagt gtcgatgaag ccgggtgaaa
     5221 ccaggcagcc tttggcatcg atctttgtgt ctatggcttg agagtagtct ttgccgacga
     5281 attcgatccg attgccatcg tagacgaccg aaccgtcctt gatgatctca tgttgtgttc
     5341 catcaaatcc gatgacaaag ccgccttgaa ttaaagtctt catgccgaac ctttcactca
     5401 aatttcgaca cgggcaggac taacaaaaga atttgtctaa gacaagctat ggccctaagt
     5461 taatcctcga agagaatcgc tcttgggctc atttgcatgg agatgtttta acctaacgag
     5521 cttctctgat tctaagtaac ctacttgtcg ggtaagtatc tttaatttgc attattatgg
     5581 cgctttgccc agggagggtc tttcagaaga gatggtccga gaatgactcg taggtcaatg
     5641 gcgcaggaga taacgactcg atcgctaatt aaacggcaaa gcggaagtga acgatgtcac
     5701 cgtccttgac gacataatct ttgccttc
//
LOCUS       DASUTG010000487         3998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1705785, whole genome shotgun sequence.
ACCESSION   DASUTG010000487 DASUTG010000000
VERSION     DASUTG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705785"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="VF089_11125"
     CDS             <1..2025
                     /locus_tag="VF089_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6174557.1"
                     /translation="LYASGGAAFGAGYSDVYVSLPALRVLTFAALIAAALSAAQIMRP
                     GFGYVLVGIAGLVLVHVIGLSAAPAMVQRFHVVPNEVDSERPFIEHNIKFTRTAYGLD
                     KIENEDFPAEENLNAADLQRNDATIKNIRLWEHQPLLATYAQLQEIRTYYKFVDVDND
                     RYNIDGTYRQVMLSARELSHQHLPSRIWINEHLTYTHGYGVVFGPVNQVTPEGLPEFF
                     IKDIPPVSDGSIKVTRPEIYYGELANDYVFVKSGARELDYPAGDQNIYTQYSGSGGVS
                     IGSLWRKLIFSAHFATLRIALSEDVVQESRILYQRQIQERVKKVAPFVTFDRDAYMVI
                     AQGGRLFWMIDGYTTSDRLPYSAPIRRQGTNYIRNSVKAVIDAYNGTVDFYMSDSEDP
                     IVQSYQKIFPELFKPLEQMPDDLRAHIRYPEDLFAIQAQMYATYHMRDPQVFYNKEDL
                     LSIPRQTVSGTEREMAPYYTIMRLPGETKEEFVLLLPFTPNNRDNMRAWLAARSDPPH
                     YGKLIALDFPKAKLVYGPKQIDARIEQDAFISQQLSLWGQRGQVIRGSLLAIPIEKSL
                     LYVQPLFLAAEKGSLPELKRVIVAFGNQIAMEETLDQSLQRVFGGKASSEPTPMPASL
                     KEAMIESSLAQRALEHFSRSQESLRQGNWAGYGEEQKKLEALLREMQQRQ"
     gene            2250..2978
                     /locus_tag="VF089_11130"
     CDS             2250..2978
                     /locus_tag="VF089_11130"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6174558.1"
                     /translation="MTGQHRQLAIRGLIFVVLVGLLFLFSRYIPQPFTALVPPGDEVD
                     DFEGIYSSSDKLGKFLRSLGPYSPAVFVLFQVSQVIAAPFPGELTGVAGGYVYGETLG
                     FILSTIGLTLGSWVAFELASILGRPFVERFVKQDVLHRFDFLTTNTGAAICFLLFLFP
                     GFPKDYLCYVLGLSRMKLATFLFVTFVGRIPGTYFLTMQGASIRNQEYYTAIIFAVVS
                     AVILLMAYLYRGRIFNWMKHRHEI"
     gene            complement(3025..3849)
                     /locus_tag="VF089_11135"
     CDS             complement(3025..3849)
                     /locus_tag="VF089_11135"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX6174559.1"
                     /translation="MDGWGIAVILGVVEGLTEFIPVSSTGHLIIASHLLGFFGEKAAS
                     FQVAIQFGAILAVLLLYWRRFADLLPLVKNDSRCKNSNLAGWAGIWRITAATLPALVV
                     GYLTKDFIKTYLFTPWAVTVALAGGGVAILIAERFIRRRASKPLETVTIVQAIGVGLF
                     QVLALWPGTSRSAATIVGGMLLGLDRKGAAEFSFLIAVPVMLAIECYELIKMGSLFSV
                     QDATNFLIGFVVSFIVAVLAVKTFILFLSRWTLVPFAWYRIIVAPIFFLLTNETRL"
BASE COUNT          955 a         1050 c         1016 g          977 t
ORIGIN      
        1 ctgtatgctt ccggcggtgc ggcttttggc gctggttact ccgatgtcta tgtaagcctg
       61 ccggccttga gagtcttgac gttcgctgcg ttgatagcgg ctgcgctttc cgcagctcag
      121 atcatgcgcc cggggtttgg ctatgtgctt gttggcatag ctggattggt tctggtccac
      181 gtgattggac tcagcgccgc gcctgcgatg gttcagcgct ttcatgtagt tccgaatgag
      241 gtggattcgg aacgtccctt catcgaacac aacattaaat ttacccgaac agcctatggc
      301 ctggataaga ttgaaaacga ggattttccg gctgaagaaa acctaaatgc cgccgacctc
      361 caacgtaatg atgcgaccat caaaaacata cgcttatggg aacatcagcc gctactggcg
      421 acctatgcac aattgcagga aatacgcacc tactacaagt tcgtcgatgt cgacaacgat
      481 cgctataata tcgatggcac ctatcgccag gtcatgcttt ctgctcgtga actttcgcat
      541 caacacctac caagccgcat ttggatcaac gaacatttaa cctacacgca tggttacggc
      601 gtcgtctttg ggcccgtgaa tcaggtcacc ccggaagggc tccctgagtt tttcatcaag
      661 gatataccac cggtctcaga tgggagtatc aaggtgacgc ggccggagat ttattacggc
      721 gaactcgcca acgattacgt gttcgtcaag agcggcgcac gagagctgga ttatcctgca
      781 ggggatcaaa atatctacac tcaatacagc ggcagcggtg gcgtgtccat cggctccttg
      841 tggcgcaagc ttatcttttc ggcacatttt gcaactttga ggatcgcgct ctcagaggat
      901 gttgttcaag agagccgaat tctttatcag cggcagattc aagaacgggt gaagaaggtc
      961 gctccgttcg tcaccttcga tcgggacgcg tacatggtca ttgcccaggg cggcaggcta
     1021 ttctggatga tcgacggcta cactacttcg gatcgattgc cgtactcggc gccgatacgc
     1081 cgtcaaggca caaactatat ccgcaattcg gtgaaggcag tgatcgacgc gtacaatggt
     1141 accgttgatt tttacatgag tgattccgag gacccgatcg ttcaatccta tcagaagatc
     1201 ttcccggagc tttttaagcc gctcgaacaa atgcccgacg acctgcgcgc gcacatacga
     1261 tatcctgagg atcttttcgc gattcaagct cagatgtacg caacgtatca catgcgggat
     1321 ccgcaggttt tctataacaa agaagattta ttaagcatcc cgcgacagac cgtaagcggc
     1381 acggagcggg aaatggcccc ctactacacg atcatgcgtc taccgggcga aacgaaagag
     1441 gagttcgttc tgcttctgcc gttcacgcca aacaatcgag acaatatgcg agcatggctg
     1501 gcggcgcgca gcgatccacc ccactatggc aaactcatag ccctggattt tcccaaggcg
     1561 aaactggttt atggtccgaa acagattgac gcgcgtatcg aacaagacgc ttttatctcg
     1621 caacaactga gtctgtgggg acagcgggga caggttatcc ggggaagctt actggccatc
     1681 ccaatagaga agtccctgct ctatgtccaa cctctatttc tggcggcaga aaagggttct
     1741 ttaccggagt taaaacgagt catcgtggcc tttggtaacc aaatcgctat ggaagagacg
     1801 ctcgatcaat ccctgcaacg cgtgtttggg ggaaaagcaa gtagcgagcc gacgccgatg
     1861 ccggcgagcc ttaaagaagc gatgatcgag agtagcctcg cccaacgcgc cctggagcat
     1921 ttttcccgtt cgcaagaatc tttacggcag ggaaattggg ccgggtatgg tgaggagcaa
     1981 aaaaagcttg aggctctgtt acgtgagatg cagcaacgcc agtaatcagc gcactgctgt
     2041 tcttgcaact tcgcaaaggg ttgactgagt gggagcgttt cccaagcgca tcaccatcat
     2101 agataatcct tcatcaggca tcgctcagtc tagattggct gcttcgaaat actcgggacg
     2161 cgtggctgtc acaactcacc tgtcgtcggc ttttgatagc cggtgtcatc gacctcgttt
     2221 cttatgaatt ttgttgttat aaagccgcga tgacgggtca acaccgacag ctcgcgatca
     2281 gaggtttgat tttcgttgtt ctggtcggcc tgttgtttct ctttagccgc tatataccgc
     2341 agccgtttac cgctctggtt ccgccgggtg acgaggttga cgacttcgaa ggaatctatt
     2401 ccagcagcga caagttaggc aaatttcttc gttcactcgg cccttattcg ccggcggtat
     2461 ttgttctctt tcaggtctcg caagtcattg ccgcgccgtt tccaggcgag ctgaccggag
     2521 tggcaggtgg ttacgtttat ggcgagactc tgggtttcat tctctcgact ataggcctta
     2581 cattgggttc atgggtcgct tttgagttgg caagcattct aggcagaccg tttgtcgaac
     2641 gctttgtcaa acaagatgtt ttgcaccggt tcgacttttt aaccaccaat accggagcgg
     2701 cgatctgctt cctgttattt ctgtttccgg gttttccgaa agattatctc tgctatgtgc
     2761 ttggcctgag ccgaatgaag ctggcgacgt ttttgtttgt gacgtttgtt ggcagaattc
     2821 cgggcactta ctttctcacc atgcaaggcg ccagcattcg aaatcaggag tattatacgg
     2881 cgatcatttt cgccgtcgtc tcggcggtta ttttgctaat ggcatatctc tatcgcggtc
     2941 gcattttcaa ctggatgaag caccgccacg aaatctgaac ctgcaaattg cctctgccaa
     3001 gcttttccca aacgccgcaa taacctagag cctcgtttca ttggtgagta gaaagaaaat
     3061 cggagcaacg ataatgcggt accacgcaaa cggcacaagt gtccacctac tcaggaagag
     3121 aataaatgtc ttcacagcta aaaccgccac gataaaagaa acgacgaatc caatcaggaa
     3181 attcgtcgca tcttggacgc taaacaagct gcccatttta atcaattcat aacattcaat
     3241 ggcaagcatt accggcacgg cgatcaaaaa ggaaaattcc gcggctccct ttcggtcaag
     3301 acccaaaagc atgccgccta cgattgtggc agcggatcgt gaagtgccgg gccacagggc
     3361 aagaacctga aatagcccca ctccgatggc ctgaactatc gtcacagttt ccagaggctt
     3421 cgatgctctt cgcctgatga aacgttcggc gatcaaaatc gctaccccac cgcccgccaa
     3481 agcgacagtt accgcccacg gtgtgaaaag ataggttttg atgaaatctt ttgttaagta
     3541 gccgaccacc agggcgggca acgttgcggc ggttatccgc caaatcccag cccatccggc
     3601 gaggtttgaa tttttgcatc ggctgtcgtt cttcaccaga ggcagcaaat cggcaaagcg
     3661 acgccagtag agcagcagca cggctagaat agcgccaaat tgaatcgcaa cttgaaaact
     3721 tgcggctttc tcaccgaaga agcctagaag atggctggcg atgataagat gcccggtcga
     3781 tgatactggg atgaattcgg ttagcccctc aaccaccccc aagattacgg caattcccca
     3841 cccgtccatg tattcccctt gttagtttac ttgccgttcc taagcccgaa ctacagcgct
     3901 tgctattcat gcgcatgcgc gggattgtcg gccaggtgac gattcgacat caaaacgtac
     3961 tcgttaacgc ttgtcagcct gcgcggcgaa gctccgat
//
LOCUS       DASUTG010000488         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_223899, whole genome shotgun sequence.
ACCESSION   DASUTG010000488 DASUTG010000000
VERSION     DASUTG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223899"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VF089_11140"
     CDS             complement(<1..807)
                     /locus_tag="VF089_11140"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6174560.1"
                     /translation="MSIAYNPSDNFEVKVSDVEFRRAPTRTLMARIYQPQGNGPFPAL
                     LDAHGGAWNNQDRTANAPMDETLAASGLLVVAIDLTVAPEAPYPASVQDANYGVRWLK
                     ANAAKWNGDPATVGALGSSSGGHVMELCAMRPHDPRYRAHSLPEAADIDARLAYLATR
                     SPVSDPFTRFNHAEKMGREEMVRYTKNYFNPWETIHEGNPQEILDRGETVTLPPLLIM
                     QGELDDNVLPSAQEKFAASYRAAGGDCELEIFAGCEHRWVAEPGPQTDRAA"
     gene            complement(971..1165)
                     /locus_tag="VF089_11145"
     CDS             complement(971..1165)
                     /locus_tag="VF089_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX6174561.1"
                     /translation="MADRYKVDCRKFPSDQGCTVTISGSIDEVVELGWLHAKTHHAHK
                     DDEETGIKEWIRGNAEAAND"
     gene            1369..>2154
                     /locus_tag="VF089_11150"
     CDS             1369..>2154
                     /locus_tag="VF089_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174562.1"
                     /translation="MRKKSLYVGLLALSVVLVFSGSASAQSSKLSQLKLAVSTATPHN
                     TPLWVAKDKKIFEKYGLDVQMLFVMGGSLVSQMLAAGEIQVAANAPAALVSLIAGGAD
                     IALFLGISNTSPFALVTQPAIKTAADLRGKRLGTARFGGSSHISALIALDHLDLDPKR
                     DRIILMQTGLDPERMAALEQKGLDAAMLQRLATKTMISKGYNELLNLTRAKIPYQNTV
                     LASRRDHMAANPKLYDSFIRGVVEGYAYVFNKDNKQAVKEVLAR"
BASE COUNT          462 a          574 c          609 g          509 t
ORIGIN      
        1 agccgctcga tcggtctgcg gtccaggttc agcgacccac ctgtgttcgc agcctgcgaa
       61 aatctccaat tcacaatcac cgcccgccgc gcgataggat gcggcgaact tttcctgagc
      121 ggacggcaga acattatcgt ccagttcacc ctgcatgatc agcagtggcg gaagggtgac
      181 cgtttcgcca cgatcgagaa tttcttgtgg gttgccttcg tggatggtct cccatggatt
      241 gaaataattt ttagtgtatc gcaccatctc ctcgcggccc attttctcgg cgtggttaaa
      301 ccgagtaaaa ggatcactta ccggcgatcg cgtcgccaag taggccaacc tggcgtcgat
      361 atctgcggct tccggcaatg aatgcgcgcg gtagcgcgga tcatgcgggc gcatggcgca
      421 aagctccatc acatggccgc cgctggagct gccgagcgcg ccgaccgtag ccggatcgcc
      481 gttccacttg gcagcgttcg ctttcagcca gcgcacccca tagttggcgt cttgcactga
      541 agccgggtat ggcgcttctg gcgcaacggt gaggtcaatg gcgacgacga gaaggccgct
      601 cgccgccagc gtctcgtcca ttggagcgtt ggctgtgcgg tcctgattgt tccacgcgcc
      661 gccgtgtgca tctagcaatg ccggaaatgg cccattgcct tgcggctgat agattcttgc
      721 catcaaggtt cgcgtcggag cgcgacgaaa ttccacatcc gaaaccttga cctcaaagtt
      781 atccgaggga ttgtaagcaa tgctcatagg gcctcctggc acgcgctatg cgcgtgttca
      841 atagttcaac agttcaaaag tttaacgatg actctgttgg agctgtcaaa acggtcgaat
      901 cgttgcggca gctgtgagcg cgcgccgcag ctgcctgctg agctatcatt ccgaaaggga
      961 tactcgaaat ctaatcgttg gctgcttcag cgttaccgcg gatccattcc ttgatgccag
     1021 tttcttcgtc gtccttgtgc gcatggtgtg ttttggcgtg gagccatccg agctcgacga
     1081 cttcgtcgat cgatcccgag atggtcaccg tgcagccctg atcgctggga aattttctgc
     1141 agtcgacttt gtatcgatcg gccattggat caatctcctt tcgccaagcg tggtttgcat
     1201 gcttctgcca tagtaccggt tgccagcaat tgcaaccgga atttcgttgt gaggctgaga
     1261 caaagccggc cgatcggctt tgcggggaag gtgaggacca ctgttcgggc gccgaaatat
     1321 atgattgatt aaatcaaggc aatatttaaa tctggagagg aacaggtaat gagaaaaaaa
     1381 tcgctatacg ttggattgtt ggcgctttcc gtagttctcg tgttcagcgg atctgcgtcg
     1441 gcgcaatctt cgaagctgtc ccagctaaaa cttgccgttt ctactgccac gccgcataac
     1501 accccattat gggtggccaa ggacaaaaaa atattcgaaa agtatgggct cgatgttcaa
     1561 atgttatttg tcatgggcgg atcgctcgtc agccagatgt tggctgcagg tgaaatccag
     1621 gtggcggcaa atgcgccggc cgctctggtg agtttaattg ctggaggcgc cgacatcgct
     1681 ctgttcctgg gcatcagcaa cacgtcacct tttgccttgg tcacccagcc tgcgatcaag
     1741 accgcggcag atctcagggg aaagcggctc ggcacggcac gcttcggcgg ctcttcgcac
     1801 atttcagctt tgattgctct ggaccatttg gacctggatc cgaagcggga caggatcatt
     1861 ctgatgcaaa ctggcctgga tccggaacgg atggcggcgc tcgagcaaaa gggtcttgat
     1921 gccgcgatgc tgcagcggct tgccactaag acaatgatca gcaaggggta taatgaactc
     1981 ttgaacctaa ctcgcgcgaa gattccgtac cagaatactg tcctggcctc acgcagagat
     2041 catatggcgg cgaatccgaa actctatgac agcttcattc gtggcgtagt cgaaggatac
     2101 gcttatgtct ttaataaaga taacaagcag gcggtgaagg aagttctggc gaga
//
LOCUS       DASUTG010000489         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1410127, whole genome shotgun sequence.
ACCESSION   DASUTG010000489 DASUTG010000000
VERSION     DASUTG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410127"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF089_11155"
     CDS             complement(<1..458)
                     /locus_tag="VF089_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174563.1"
                     /translation="MAVESAASQTIQSKGPLVWLNMDQKELDDAYNQSVYAPNQEQVA
                     KRRRLASEAAIKRLAPALRLSYGPTEIEKLDIYRTSRPNAPIMVFTHGGAWRNGSAKD
                     FAFPAEVFINAGAHYVVLDFAAVQDVGGNLMVMADQVRRAVAWVYKNAES"
     gene            complement(743..1099)
                     /locus_tag="VF089_11160"
     CDS             complement(743..1099)
                     /locus_tag="VF089_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174564.1"
                     /translation="MPEQITKYPDVTLQVLSEAGAVCGKGAPQKILKQCPAERFCSLP
                     TGEMCIYGIKEIPRMTQIAPAELAQVVCPLTPKGASFAAGFSGLEAAMIGAVFLGGLA
                     LGKIWRRPRDGAQRPR"
     gene            complement(1509..2855)
                     /locus_tag="VF089_11165"
     CDS             complement(1509..2855)
                     /locus_tag="VF089_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX6174565.1"
                     /translation="MRNLKLKSTIALSLALSACSLAPQYERPQMVMPVGWTNVAGVGA
                     SDQPATVPFWQELGSDELNNVMKIALAQNLDLEAALHRIDQARAQTRIAGSSLFPQIE
                     ASGGASRSFEDPRNVSTARGVGSISYEADLWGRNRSNRTAANYRAEATAFDRDALELV
                     VTSDTADFYSQVLSLNERIRIAESNLKNAEEVLRIIEARYKAGSVSGLEVSQQRVAVN
                     NLRASLASLNQQRSTTLNALAILLGQAPQNLQAPRDTLASLKIPDVNLTPPATLLTAR
                     PDIERAEASLRAANADIGAARAAFFPSLNFGLDASIAAGFGGPAATAVSLASSALAPI
                     FTGGRLRGNLEDVTARQKELAAQYQKTVLVAFQEVEDALASYKNVNEQAVIARESVKE
                     SQAAYDIAKARFDAGAIDYLNLLVTQRSLYEEQDNQISVYQDQIAAFVQLRKALGS"
     gene            complement(2861..>4417)
                     /locus_tag="VF089_11170"
     CDS             complement(2861..>4417)
                     /locus_tag="VF089_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174566.1"
                     /translation="LLQRLGLGDRTDHRPAELSGGQQQRVAIARALVNDPPVILADEP
                     TGALDSKSGDEVLSLLKQLHAEGRTIILITHAENVAQHASRIVRIQDGRILDDSGVVS
                     KPSPMPVEAADLNLKSAVTLAASMQEALVTAWRSLRVNLFRTVLTLLGIIIGVAAVVA
                     MLAVGEGSRQKVLDRISSFGTNLMLIRPGAAGIRNAGDIVTLIPEDAAAIKALPNIET
                     ALPERSGRITVRFGNRDYQTSVQGTGEDFPQARDWKVAEGQFFNADDMKQYAPVVVLG
                     RTVAKTLFPDGGDAVGKYVLLKNVPFLVIGIMTEKGASPNGSDQDDVIFTPITTALVR
                     LFGKSYLSSITVKVSDTADIAATQERVENLLRERHRTEDFSVRNMASYLQAAIETQDT
                     FTLLLGTVAAISLLVGGIGVMNIMLVSVVERTREIGIRMATGARMRDILLQFNIEAAV
                     VCAAGGLLGILVGVAAGMILRYSGMSVIFSITPALLAFACASATGLIFGYLPARKAAR
                     LDPVVALASE"
BASE COUNT          938 a         1273 c         1233 g          973 t
ORIGIN      
        1 aaactctccg cgttcttata gacccaggcg acagcgcgcc gcacctgatc cgccatgacc
       61 atgagattgc cgccgacgtc ctgtaccgcg gcaaagtcga ggacgacgta atgggctccc
      121 gcgttgataa atacctccgc cgggaaagca aagtctttcg ccgatccgtt gcgccaggcg
      181 ccgccgtgag tgaagaccat aatgggcgcg ttcggccgcg aggtcctgta gatgtcgagt
      241 ttttcgattt cagtcggacc gtaggagaga cgtagcgccg gcgcaagccg cttgattgcc
      301 gcttcgcttg ccaaccgccg ccgcttggca acttgctcct gattaggcgc atagacggat
      361 tggttgtacg cgtcatcgag ttctttctgg tccatgttaa gccagaccag cggccctttg
      421 ctttgaattg tttgagaagc ggcagattca acagccatgc ttaacccccc aactgagtta
      481 gtctcccgac gataggtcaa cttaaacctt tgttcaaggt tcaacagttc aaaagttcaa
      541 cgtaagaaaa caaaacggat cttacgccac cccggtccgt cggggtccta ggcaacggag
      601 taagttgata acgacgactt tcgcatctct catgctagga cgaatcgaag cacgtcacca
      661 tgaaaccttt tgaaacgttg gaactgattt ccgaacttcc ccgttgaacg attgaacctt
      721 tgaatcgttg aacaaagcgc gttcagcgag gtcgttgcgc cccgtcgcgc ggtctccgcc
      781 aaatttttcc aagcgcgagg ccgccgagaa aaacggcgcc aatcatcgcc gcctccaacc
      841 ctgagaatcc agctgcaaag cttgctcctt tgggtgtcaa cggacagacc acttgcgcca
      901 gttcagccgg cgcgatctgg gtcattcgcg gaatttcctt gatgccgtag atgcacatct
      961 cgcctgtggg caacgaacag aatcgttccg cgggacattg ttttagaatt ttctgtggcg
     1021 cccctttgcc gcaaacggcg ccggcttcac tcaatacctg caaggtcacg tcgggatatt
     1081 tggtgatttg ctcgggcatg caaacctcct gctcgaatgg ttccaaacgt tccagggttc
     1141 cacttgttcc agccgtccaa acgttccagt catttgaagt gcattgatca ccagccaagg
     1201 taaacatacc actcggaatc aggatggtta aacatagacg agatcgaact tcacgttgaa
     1261 gttcaacacc ggtttcaaaa ttcagttgat tcttgctgaa cggccgaggc tacgactctt
     1321 cgttcactgg aacaggcctt cggcagcgca gaaatcctcg gcagaaagaa acctatgtac
     1381 ggttatccgc ccgatcgggc ttaacggctt gacggctgga acacttggaa cggttgacac
     1441 aattcggacc ggttcgcctg cggcgctctg atcttgaacg attgaactct ataagtcgcg
     1501 attgcgactt acgagccgag cgcttttctg agttgcacaa acgctgcgat ttgatcttga
     1561 tagacggaga tctgattgtc ctgctcttcg tagaggctgc gctgggttac cagcaaattc
     1621 agataatcga tggcgcccgc atcgaaacgg gctttggcga tgtcgtaggc ggcttgtgat
     1681 tccttgacgg attcgcgtgc gatcacggct tgctcattaa cgttcttata ggaggcgagg
     1741 gcgtcttcga cttcttgaaa tgccaccagc acggtttttt gatattgagc cgccaattct
     1801 ttttgccgcg cggtgacgtc ttcgagattc cccctaagac ggccaccggt gaagatcggc
     1861 gcaagcgcgc tgctggccag cgatactgcg gtagccgcgg gtccgccgaa tcccgccgct
     1921 atgctggcat ccaggccaaa gttcaaagag ggaaagaaag ccgcgcgggc cgcgccgatg
     1981 tcggcattag ccgctcgcag gcttgcttca gctctctcaa tatccgggcg cgcagtaagg
     2041 agcgttgccg gcggggtcaa atttacgtca gggattttga gcgaagcgag cgtatcccga
     2101 ggtgcttgga gattttgcgg cgcctggccc agcagtatgg ccaaagcatt taaggtcgtg
     2161 ctgcgctgtt gatttagtga ggccagcgag gcgcgaagat tgtttacggc aacccgctgc
     2221 tgtgagacct ccaagccgga gacgctgccg gccttatagc gggcttcgat aatccgcagc
     2281 acttcttcgg catttttcaa attgctttcg gcgatgcgga tgcgctcgtt caagctaagc
     2341 acctggctgt aaaagtcggc ggtgtccgaa gtgacgacaa gctcgagagc gtcgcggtcg
     2401 aatgccgtgg cctcagcccg ataattagcg gcggttctgt tgctgcgatt tctgccccag
     2461 agatcggctt catagctgat actgccaaca ccacgggccg tgctcacgtt tctcggatct
     2521 tcaaaactgc gcgacgcacc tccgctggct tcgatctgag gaaacagcga ggagcccgca
     2581 atcctggttt gagcgcgcgc ctgatcgata cgatgcagcg ccgcctcgag gtccagattc
     2641 tgtgctagcg caattttcat tacattattc aactcatcac tgccaagctc ctgccagaac
     2701 ggcactgtcg ccggctgatc actggcgccg accccggcaa cgttggtcca acccaccggc
     2761 atgaccattt gcggacgctc gtattgcggc gccagactgc acgccgacag agccaatgaa
     2821 agagcgatcg tgcttttaag tttaagattt ctcatattac tcactctgaa gctaaagcca
     2881 ccacggggtc cagccgcgcc gccttgcgcg ccggcagata accgaagatc agtccggtcg
     2941 ccgaagcgca ggcgaaggcc agcagcgcgg gtgtaatcga gaagatcacg ctcatgccgc
     3001 tgtaacgcag aatcatgccg gcagccacgc cgacgagaat ccccaataga ccgccggcgg
     3061 cgcaaaccac tgccgcttcg atgttgaact gtagcagaat gtcgcgcatg cgcgctccgg
     3121 tagccatccg aatgccgatt tcgcgggtgc gctcgaccac actcaccagc atgatattca
     3181 taacgccgat gccgccgacc agcagtgaaa tggccgcgac ggttccgagg agcaacgtga
     3241 aggtatcctg agtctctata gccgcctgta gatacgatgc catgttgcgc acgctgaagt
     3301 cttcggtgcg gtggcgctcg cgcaataaat tttcaacccg ctcttgcgtc gccgcaatgt
     3361 cagcggtgtc agagactttg acggtaatgc tgctcaaata acttttgccg aataatcgca
     3421 ccagcgcggt ggtgatcggc gtgaagataa cgtcatcctg atcgctgccg ttgggagatg
     3481 cgcctttctc ggtcataatg ccgatcacga gaaagggtac gttcttcagc agaacgtatt
     3541 ttcccacggc gtcaccgcca tccgggaaga gggttttcgc cactgtgcgg ccgagcacga
     3601 ccacaggcgc atactgcttc atatcgtcgg cgttgaagaa ctgtccctcg gcgaccttcc
     3661 agtcgcgcgc ttgtggaaaa tcctcgccgg ttccttgcac agatgtttga taatcgcgat
     3721 tgccgaaacg gacggttatg cggccgctgc gctcgggcag agcggtctcg atattcggta
     3781 atgctttgat cgcggctgcg tcctcgggaa tcagagtgac gatatcgcca gcattacgaa
     3841 tccccgcggc gcccggacga atcagcatca gattggtccc gaaagagctg atgcggtcga
     3901 gcaccttttg ccgactgcct tcgccaacgg cgagcatcgc tacgactgcg gcgacgccga
     3961 taatgatgcc gagcaacgtc aggacggtgc ggaaaagatt cacccgcagc gagcgccaag
     4021 cggtgaccaa agcctcctgc atgctcgccg ccaaagtgac cgcgctcttg aggttgagat
     4081 cggccgcttc gaccggcatc gggcttggtt tgctgacaac gccgctgtcg tcgaggattc
     4141 ggccatcttg aatgcgcacg atgcggctgg cgtgctgcgc cacgttttca gcgtgggtga
     4201 tgagaatgat cgtccggcct tcggcatgca attgtttgag cagggacaaa acttcatcgc
     4261 cgctcttgct gtcgagagct ccggtgggtt catcggcgag aatcaccggt gggtcgttaa
     4321 ccagcgctct cgcaatggca accctttgct gctgcccgcc cgatagctcc gccggccgat
     4381 gatcggtgcg atcgccgagt cccaaccgct gcagtaa
//
LOCUS       DASUTG010000490         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1706278, whole genome shotgun sequence.
ACCESSION   DASUTG010000490 DASUTG010000000
VERSION     DASUTG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706278"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(505..921)
                     /locus_tag="VF089_11175"
     CDS             complement(505..921)
                     /locus_tag="VF089_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174567.1"
                     /translation="MSPQDSGSLGKIMLVSSEPSTEVEKCLRSAGREFVTVNDGAAAI
                     VQAQHATFRMAVLVSTGKTMDLAETVFNLRDISSSMPILIIRGRSADQDSEADIIAHS
                     SPNTRTLTVHDLEAYLGISISSTRRSFGKRHHQYKI"
     gene            complement(1219..1644)
                     /locus_tag="VF089_11180"
     CDS             complement(1219..1644)
                     /locus_tag="VF089_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174568.1"
                     /translation="MKLRTLAIFATVALILPPLDWAGAQTVLSSQEASRALSFEKLDV
                     NPSKVSGVIANKTPHTIKDVQLMVQYHWLWKDERNPGKDSPGRTVIIPLKEQLEPGES
                     QWFSYTPEFSPPSRNDGYFMPEVDVAAFTTVVRQQARAR"
     gene            complement(1871..2344)
                     /locus_tag="VF089_11185"
     CDS             complement(1871..2344)
                     /locus_tag="VF089_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6174569.1"
                     /translation="MKRKNLSTAAAIFAAASIASGTAVAQTSGTKQQNPETIRPDISA
                     PGTSTGGTQSQRTQSGAPLPKGSPFSGTVELGSPNSMQDVKAAQQALQEKGHNPGPID
                     GMMGPRTREALKSFQSASGLETTGTLNQATAEKLGVDLGPSKGSKDMNTGKSSSK"
     gene            2939..4351
                     /locus_tag="VF089_11190"
     CDS             2939..4351
                     /locus_tag="VF089_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174570.1"
                     /translation="MKPAPSKFTDLKPIEPHNKAIPRFLLIENNAEIEALLKSRFPAQ
                     ICIDTLPVPDASTGETVYDVVIWNADNSPSNKSKGFEMIQWVSRHSPETQIIVISHDE
                     GNPAGCSIRSESYHHITRPIDWNNLLVLVTQALNKCPPGQKTLSYPDVRVPVEFEGML
                     GISLPMREVFQRIIEATAVDIPVLITGETGTGKDLIAAAIHKRSERKDQPYVAVNTGA
                     MTRELMATELFGHERGAYTGAAAAHRGFFEQAHRGTIFLDEISTMEEKAQISLLRVLE
                     TKTFRRVGGEKDVLVDVRVLAATNESLEDAVKQGRFREDLYYRLDVFHIHVPPLRNRP
                     GAITLLTNQFVTVFDAIYKKNVSEVSPETYRYLRRYPWPGNVRELKNVVQRAILMSQG
                     KDFTPDLLPARIREATDSQAGPDMQKMPIHVGMTLDAVEKEFIRMTLESTGGNKKEAA
                     AVLSISRRALYNKLQKYGLM"
     gene            complement(4355..>4618)
                     /locus_tag="VF089_11195"
     CDS             complement(4355..>4618)
                     /locus_tag="VF089_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174571.1"
                     /translation="FEGLVGSALYWFFTQPWAGWLVDAFHRTPFDIAGSTDQRTIILQ
                     LFVYISLVFTLLTIPVVVLLKRTLARNGIEITNEAEEKQQQER"
BASE COUNT         1100 a         1258 c         1191 g         1069 t
ORIGIN      
        1 cagcgctgat tcagtcgata aatccagtgg ttgggaaagg aagaaccagg gagagtaaac
       61 ctgtcgggga gaacgtgtgc atgaagttta catcgcagtt atgcactgtg gcactaatcg
      121 tccgatccac agtcagagag aaactcaccg cggcatgttc tcggcctctt atgtgggacg
      181 cggtaagcaa cacgcaattt tttgtgcgca gtttgtgcac gcggagttgt cgagaagatg
      241 attcactagc gcaactacaa aagtgaatca ggatgcgcgc tcactcaagg atcgcagcgc
      301 ctgcaagagg tcacctgcga tccatttgcc aagtattgaa ggtttgatga cgactcagtc
      361 cgggcaggtc gctttccagg atgaagccgc ttgccaaaac gttccggaat gctgaacggc
      421 aaccaacatt aacaattgtc cgacgatcag cccaccgact agcacttcta ggcttcttgg
      481 gttcatcgct ccggcttgcc caatctaaat cttgtattgg tgatgcctct tgccgaagct
      541 tcgtcgagtc gaacttatgc tgatgcccag ataggcttcc aggtcatgga cggtcagggt
      601 ccgtgtattt ggtgaagaat gagcaatgat gtcggcttca gaatcttggt cagcagatcg
      661 ccctctgatg ataaggatgg gcatcgatga acttatgtct ctaagattaa aaaccgtttc
      721 agccaggtcc atcgtttttc cggtcgagac caggactgcc atcctgaagg tagcgtgctg
      781 cgcctgaacg attgcggccg cgccatcgtt caccgtaacg aactcacgtc cggcgctgcg
      841 caggcatttc tccacctccg ttgatggctc cgaagaaacc agcatgatct tgcccaagct
      901 gccggaatcc tgtgggctca tcgattacaa ctctgattgc gcatcgtatg aagcacactc
      961 actcggagag caatcggaat accaaacgtg ctggtcgatg aggggacgag aacaacggaa
     1021 gttgaggttg cccacaagaa cgaaactatt tcctgggcgt gtggcatcga cttactgcta
     1081 tccgagttga gatcatgagc cgggagaaag tcttgcaccg gcacgccaat ctcgtggggc
     1141 gcccttgaga tctctttcga acctcgccag tctagcagca gagaatcaaa cagattggat
     1201 gcttctccat tctcttcctt aacgggcgcg agcttgttgt cggacaaccg tcgtgaaagc
     1261 tgcaacatct acttcgggca tgaaatatcc gtcgttcctt gacggcggtg aaaattccgg
     1321 tgtgtagctg aaccactgtg attcacccgg ctcaagttgc tccttcaatg ggatgataac
     1381 cgtcctgcca ggcgaatctt tcccggggtt tcgctcatct ttccagagcc aatggtattg
     1441 aaccatgagc tggacgtctt tgatcgtgtg cggcgtctta ttggcgatca ctcccgaaac
     1501 tttggatgga ttcacgtcca gcttctcgaa gctcagtgcc ctggaagctt cttgactgct
     1561 caacacggtc tgagcgcctg cccaatccaa gggcggcaag atcaaagcca ctgtagcgaa
     1621 aatagctaac gtgcggagct tcatgatctg tcctcccttc ctttaggtct tcgtttaatt
     1681 tccgttttcc gaactatcgg agagtcccgc gaggccaaga tgtctggtta gcgcaacggt
     1741 agatcccgaa ctcggtagga cgcaacgcca caccgcttca gagatacagc cagataaccg
     1801 gctctcgcaa gactccctcg gcgagcggcg ctattcagcg ccgctcgatc ggttaaacta
     1861 tctcacaaac ttattttgac gaactctttc cggtgttcat gtctttggag cccttcgaag
     1921 ggccgagatc aacgccaagc ttttctgccg ttgcctgatt gagcgtaccc gtcgtctcca
     1981 gtccggacgc ggattgaaat gacttcagcg cttctctcgt gcgcgggccc atcattccgt
     2041 cgatcgggcc cgggttatgc cctttctcct gcagggcctg ttgagctgct ttgacgtcct
     2101 gcatagagtt tggactcccc aactcgaccg tgccgctaaa gggggagccc ttcggcaggg
     2161 gcgctcccga ctgggtgcgc tgcgattgcg tcccgcctgt tgaagtcccc ggcgctgaga
     2221 tgtcgggccg tatcgtctcg gggttctgtt gtttggtgcc cgatgtttga gccacggccg
     2281 tgccggaagc aattgaagct gcggcaaaga tcgcggctgc tgttgaaaga ttttttcgtt
     2341 tcatagcttt cctccgtact agtttcaccc tgcaaggcgc aatggttgtg ccagccacgc
     2401 gcttcgaaaa taccgcgggc aatccccatg tactgcgggc gcttctccca tcgtgaactg
     2461 agcatgtgca aaacaatcac actgggccat cacatgcacc gatttctaca cagagcttcg
     2521 ctgccgaacc gacaatggtg ctttaagttc ccaatgcggg ttgactgata gcgccgcgcg
     2581 cacaatttcc cgagtcattc acttaagttg gttgctaaga gtaacgcgat agggccacgc
     2641 ccggaccggt tagcttgcaa gaccggcatc ccgagcattc gagcactggc aatcaatcac
     2701 catattcctc acgagatctt cctccgccga ccaaacagcg gcttactcaa ataaattttg
     2761 cctggtagct tgccttcgtc cggatattcg ttagttttaa aggagaatga gtttattcct
     2821 gccgggacaa tacgatggaa agagcgtggg ctgctggaaa atgcttcggg tttttagctg
     2881 cgtcgttaga gcggcgaaag tgcttggcta aaggtcactg caataaacct ttgatcgcat
     2941 gaaacccgct ccatcaaagt ttactgattt aaagcctatc gagccacata acaaagcaat
     3001 cccaagattt ctgcttatcg aaaataacgc cgagatcgag gcgctgctca agagccgctt
     3061 ccccgcgcag atctgtatcg atactctgcc ggtcccggac gccagtacgg gagaaactgt
     3121 ttacgacgtt gttatttgga atgcggacaa ttccccatca aacaaatcta agggatttga
     3181 gatgattcaa tgggtttcga ggcactcccc ggaaactcag atcatcgtca tctctcatga
     3241 tgaaggcaac ccggcgggct gcagcataag atcggaaagt taccaccata ttacccgccc
     3301 aatcgattgg aataatcttt tggttttagt gacacaggca ctgaacaagt gtcctcccgg
     3361 ccaaaagact ctctcgtacc ccgatgtccg cgtgccggtg gaattcgaag gcatgctggg
     3421 catcagtttg cccatgcgcg aggtattcca gcgcatcatc gaagccacag cagtggatat
     3481 cccggtgctg ataaccggag aaaccggaac aggaaaagat ctcatcgctg ctgccatcca
     3541 caagcgcagc gaaagaaaag atcagcccta tgtcgccgtc aacaccggtg ccatgactcg
     3601 cgagctaatg gccaccgaac tttttggcca tgaaagaggc gcttacacag gagcggcagc
     3661 ggcgcaccga ggcttttttg aacaggcgca tcgggggacc atctttctcg acgagatcag
     3721 cacaatggag gagaaagccc agataagtct tctccgggtt ttggaaacga aaacgtttcg
     3781 tcgagtagga ggagaaaaag acgtcctcgt cgatgttcgg gtgctcgccg ccaccaatga
     3841 aagcctcgag gatgcggtca agcaggggcg cttccgcgag gacctttact atcgcctcga
     3901 tgtttttcac attcacgtgc caccgcttcg aaatcgtccc ggagcgatca ctttgcttac
     3961 gaatcaattc gtaaccgtgt tcgacgctat ctacaagaaa aacgtcagcg aggtttcgcc
     4021 ggaaacttat cggtacttga ggcgctatcc gtggcccggc aatgtccgcg aactcaagaa
     4081 cgtggtccag cgggcaattc tgatgtccca ggggaaagac ttcaccccag accttctccc
     4141 cgcaagaatc cgggaggcca ctgactccca agccggacct gatatgcaaa aaatgccgat
     4201 ccatgtcggc atgactttgg atgcggtcga aaaggaattc atacggatga ctctcgagtc
     4261 caccggcggt aacaagaaag aagccgccgc agtcctcagc atcagcaggc gggcgcttta
     4321 caacaaattg cagaaatacg gcttgatgta attctcaccg ctcttgttgc tgcttctctt
     4381 ccgcctcatt agtaatctca atgccattgc gcgcaagagt gcgttttagc aaaaccacca
     4441 ccggtattgt cagcagcgta aaaaccaggc tgatatagac aaacagctga agaatgatcg
     4501 ttcgctgatc ggttgaaccc gcaatatcga acggcgtgcg atgaaacgca tcaaccagcc
     4561 aacctgccca gggttgcgta aagaaccaat aaagcgcgct tccaactaag ccctcgaa
//
LOCUS       DASUTG010000491         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1557201, whole genome shotgun sequence.
ACCESSION   DASUTG010000491 DASUTG010000000
VERSION     DASUTG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557201"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..486)
                     /locus_tag="VF089_11200"
                     /pseudo
     CDS             complement(269..486)
                     /locus_tag="VF089_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020862330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            617..970
                     /locus_tag="VF089_11205"
     CDS             617..970
                     /locus_tag="VF089_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174572.1"
                     /translation="MIRFQIIERTGVNLHKTLIDAMRAGELRTFEATKRGRRIQHKNI
                     LYPGWMNWSVHDGMINCEVISPRKPGREWQLFSAFLGRLADRYADQIIGINIQFPTEP
                     SKSPKSTVKKRRRKS"
     gene            complement(1027..1905)
                     /locus_tag="VF089_11210"
     CDS             complement(1027..1905)
                     /locus_tag="VF089_11210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6174573.1"
                     /translation="MTNDVLVHSVKLRPGLPSDIEPCGRIVFDAFATIAAQHNFPPDI
                     PSIEVGTELMTLLLTHPHFFSVVAEVNGKIVGSNFLDERSAIAGVGPRTIEPTIQNRG
                     LGRRLMEAVLNRASEKAHTGVRLLQSAYHGRSLALYSTLGFQVREGIACMQGSPIGAS
                     LAGYPVRAAREEDLEACDRICRLVHGHDRHRELLDAVQLGTAKIVEHGGRITGYATDF
                     GFFGHAAAETTRDLIALISSASAFTGPGILVPMRNAPLFQWCLSQRLKVVQVLTLMTR
                     GLYNEPAGAYLPTVLF"
BASE COUNT          476 a          565 c          595 g          498 t
ORIGIN      
        1 ctaactatta aggagccgtt tagggcgcgc cgttgctggc cgcctagtcc aaacagctct
       61 acacgagttg caagattgct tgttgaatta tatcggcacc tgacatgaaa gctgagttgc
      121 gtgactgtgg gagcgttaat ctcaagccgt tggttaaccc ttcgtcgtta cgaggtggtt
      181 gttgaagtca ttcaacggag tgaatgttta gatatccccg cactttcagc tgcataccat
      241 gcagcgggtt gttagccggg tccttccgct attcggggta ctcgtacaca ggggtctgaa
      301 tcactcggtt cggtttaccc ggcccgagaa ccgttctctg cagtcgctcg atggtgtagg
      361 ggagaaaaat tggtcgccta tctcgtcgac tcgggccggc acgttctcta caatgtagaa
      421 cttcccctca tgctccagcg tgtacgtcac gcgccgctca actagtttct ctgttctatc
      481 acttatgatg ttctccgtac atcgccttgc tcgtccagaa ataatcccgc cgctttcggt
      541 ctgaccaagt ataatggcgt tcgttattgg cgtcagacct gtaccggaac taagtattgc
      601 agaggaggtg tcgagtatga tccgctttca aatcatcgaa cgcacgggag taaatcttca
      661 taagacactc atcgatgcca tgcgggcggg tgagctgcgc actttcgaag caaccaagcg
      721 cgggcggcgc atccagcaca agaacatact ttacccggga tggatgaact ggtctgtcca
      781 cgatggcatg atcaattgcg aagtgataag tccgcgcaag cccggtcgcg agtggcagtt
      841 gttcagtgcc ttccttggtc gcctggccga tcgatacgct gaccaaatca tcggcatcaa
      901 tattcaattt cccaccgaac cttcgaagtc ccccaaatca acggttaaga agcgccgccg
      961 caaaagctga cggcgcgcgc cgtaaaccat tgatggcatc tgcggattgc ttatagcgcg
     1021 gtgtggttaa aacagcaccg tcggcaaata ggctcctgcc ggttcattgt aaagcccgcg
     1081 cgtcattagc gtcagcacct gtacgacctt caagcgctga ctcaaacacc attgaaatag
     1141 tggtgcgttg cgcattggaa caagaatgcc tggaccggta aaagcagaag ccgaactaat
     1201 cagcgcgatc aggtcgcggg ttgtttcggc cgcagcatgt ccaaagaaac caaagtcagt
     1261 ggcgtaaccg gtgattcgcc caccgtgttc gacgatcttc gctgtgccaa gctgaacagc
     1321 atcaagcagc tcgcgatgac ggtcgtgtcc atggaccaaa cggcaaatgc gatcgcaagc
     1381 ttcgagatct tcctctcgcg ccgcgcgtac agggtagccg gccagcgagg cgcctattgg
     1441 ggagccttgc atgcaagcaa ttccttcgcg cacttgaaag cccagcgtac tgtatagcgc
     1501 gagcgaacgg ccgtgatagg cggattggag cagtcgcacg cctgtatggg ctttttcgct
     1561 ggcgcgattg agaacagcct ccatcagccg gcgcccgagc ccgcggttct ggatagtggg
     1621 ctctatggtc ctggggccca cgccggcgat tgcagagcgt tcgtcgagaa aattgctacc
     1681 aacgattttg ccgttgacct ctgccacgac actgaagaaa tgcggatgcg taagcaacag
     1741 ggtcatgagt tcagtaccga cctcgatgga gggaatatca ggtgggaagt tgtgctgggc
     1801 ggcaatcgtg gcaaacgcat cgaaaacgat ccggccgcat ggctcgatgt cggaagggag
     1861 tcccgggcga agtttgactg agtggacgag cacgtcgttg gtcatgtgtc tatcctctat
     1921 ggtctgagct gaaagcccaa tcgattgggc gtgctctacg tattctgctg aagtttctgc
     1981 caaagattga tccgacaaat tcacgacgtg tcaacggcgt ttgcgtttaa cagcgcctgc
     2041 tgttcaggtt ttgctcagtc gaatggacag gtgggtactg gtttggctct cgctaacgcg
     2101 agaaagtttc aatcgggatc cttcgttcta acac
//
LOCUS       DASUTG010000492         5607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2002375, whole genome shotgun sequence.
ACCESSION   DASUTG010000492 DASUTG010000000
VERSION     DASUTG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002375"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1134)
                     /locus_tag="VF089_11215"
     CDS             complement(25..1134)
                     /locus_tag="VF089_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEX6174574.1"
                     /translation="MPAIEQNKLKRAGTGLRAVDHDRACPGFTLFAPMSGAGNIYLID
                     LDGKLIHRWQMPYPPGNYGYLTERGTLFYNGKTAEDSERFISRQPWKGGAVLEADWNG
                     RVLWEVHHPDHHHDGIRLRNGNVLLLCLDRLPQNLVAKVKGGMPGTEHHGKMYADYLV
                     EMTTEGDVVWEWHTWEHLDPEADCITAIQERREEWAHGNGVAEIPNGDIVVSFRTIST
                     VIIIDRKTGKIVWKLGAPPLCGQHAPTPLPNGNLLIFDNGPHRLDHVMPFSRVIEVET
                     ATKQIVWEYREKRESFFFSPRISNAQRLTNGNTLICEGDFGRLFEVTEGGEVVWEYVN
                     PYFGGPPNAQHNRVFRAYRYSAEEIAKARATAGAL"
     gene            complement(1294..1839)
                     /locus_tag="VF089_11220"
     CDS             complement(1294..1839)
                     /locus_tag="VF089_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6174575.1"
                     /translation="MARVPDVALEEMTAEQRRVHQEIGGARGGVVRGPFAIWLRNPQL
                     ADQANQFGNAFRVTGKLDKRLFELMVLVVARHWSAQYEWFVHERNGLAAGLTQEIVDA
                     IRTQSTPEFAREDERVVYDTITELNETRTLSQPTYDRALATLGLDLLIELIAAAGFYT
                     MVAMTLNAFDAPVPGGKRPLS"
     gene            complement(2203..3762)
                     /locus_tag="VF089_11225"
     CDS             complement(2203..3762)
                     /locus_tag="VF089_11225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6174576.1"
                     /translation="MSKSGNTSDHKSILDTNAEEALRRTNESLSLAQRAASAGVWDWD
                     LASDIVYVSPEYRELYGLAPDVPLTYDLWLNHLVHPEDRNRVDQYGREVFNSESDYRI
                     EYRILHPDRGERWVMGVGTLLRNPTGQPMRFTGINLDITDRKKAETALREANRHKDEF
                     LAMLGHELRNPLGIITTALELMRYRDVPDASLELRDMIDGQVQHMRRLIDDLLDVTRI
                     TTGKIHLEKKRCELSGIISKTVTAYHENLKASGLSLEIRLPDQPFFFTGDRTRIEQVI
                     GNLLHNASKFTESRGKVTVVLGASSDSKSAIVTVRDTGIGIDAATLPRIFETFTQADR
                     ALDRSRGGLGLGLALVKGLVELHGGEVKAESEGVGKGSTFTLRLPLDAPSISPVSREH
                     GHLAKVRPFRMVIVEDNFLAARSTQMLLNELGHQTEIAHNGTQAIALVEQFRPEVVLC
                     DLGLPGIDGYEVARRLRHCSDLSEIHLISTSGYGQEEDKQRALEAGFDAHLVKPIDFR
                     ELQRLLSELTG"
     gene            complement(5053..5541)
                     /locus_tag="VF089_11230"
     CDS             complement(5053..5541)
                     /locus_tag="VF089_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6174577.1"
                     /translation="MGIIKQWSEWWSRFLDLPDDSFRQAINILQQRYVEETQQRDRFN
                     QHADKMHYPQFREKLLQISDEKTKYADRIAEAIVALGGKLPRVPELRSTDENSWQQLM
                     IALDDENRSADHLAEQLRGIESDIPDILKLLQQISEQQKQHRGEVREMLMRSDPFAFS
                     LA"
BASE COUNT         1215 a         1558 c         1393 g         1441 t
ORIGIN      
        1 ggcttaactc gtttctcttt ttgttcacag agcacctgcc gtcgctcgcg ctttggcgat
       61 ctcctctgca ctgtatcggt aggcgcgaaa tactcgattg tgttgcgcgt ttggcggtcc
      121 gccaaaatag ggattcacat attcccaaac cacttctcca ccttccgtaa cctcaaatag
      181 tcgaccgaaa tcgccttcgc agatcagcgt gttgccgttg gttagccgct gcgcattgga
      241 aatcctcgga ctaaagaaaa agctttctcg tttttcccga tattcccaga cgatttgctt
      301 ggttgctgtc tcgacctcaa tgacccgtga aaaaggcatc acatgatcca gccgatgtgg
      361 tccgttgtcg aagatgagca ggttaccgtt cggtagcggc gtgggcgcgt gttgaccgca
      421 tagcggaggc gcgccgagct tccagacgat cttcccggtt tttcggtcga taatgatgac
      481 ggtggagatc gtgcgaaagc tcacgacgat gtcgccattc gggatctcgg ccacgccatt
      541 gccatgcgcc cattcctccc gccgttcttg aatcgccgta atgcaatcgg cttccggatc
      601 cagatgttcc caggtatgcc attcccaaac aacatcgcct tcagtcgtca tctcgacgag
      661 gtaatcggcg tacatcttac cgtgatgctc ggtcccgggc atgccacctt taacttttgc
      721 aaccagattc tgcggaagcc gatctaggca gagcagcaga acgttgccgt tgcgcaatcg
      781 tataccatca tgatgatgat ctgggtgatg tacctcccaa agaactcgcc cgttccaatc
      841 agcttcgaga acggcgccgc ccttccacgg ctgccggcta atgaagcgct ctgaatcctc
      901 cgcggtcttt ccattgtaga agagcgtgcc tcgctcggtc agataaccgt agttgcctgg
      961 aggataaggc atctgccaac gatggattag cttgccatcg agatcgatca ggtagatgtt
     1021 ccccgcgccg ctcatcggcg cgaacaaagt gaagcccgga caggcccggt cgtgatctac
     1081 cgcgcgtaaa ccggtacccg cgcgcttgag cttgttctgt tcgatggccg gcatagtcca
     1141 tctcctttcg tttgtcgatc cgatcacgtt gcgctctacg aattaaacgt ctgctggttg
     1201 gcttaactaa actcagcgtt gcatcggatg accgcgtgaa tttgggtccg gtgttgaagc
     1261 tttcgattgt ttccagaact ttcaccaatg attttacgat aacgggcgct tgccgccggg
     1321 caccggcgcg tcaaaggcat tcaaggtcat ggctaccatc gtatagaacc cggctgcggc
     1381 aatcagctcg atcagcagat ccaatccgag cgtcgcgagc gcacggtcgt aggttggctg
     1441 gctcaatgtg cgtgtctcgt tcagctcggt aatcgtgtcg tagaccactc gttcatcttc
     1501 gcgggcaaac tccggtgtcg attgcgtgcg aatcgcatcc acgatctcct gcgtgagccc
     1561 cgccgctaat ccattccttt cgtgcacgaa ccactcgtac tgcgccgacc agtgtcgcgc
     1621 gacaactagc accatgagct caaacaaccg cttgtcgagc ttgccggtta ccctgaatgc
     1681 gttgccgaac tgattggcct ggtctgcgag ttgaggattt ctgagccata tagcgaacgg
     1741 accgcgtacg acaccgccgc gagcgccgcc gatttcctgg tggacacgcc tttgttctgc
     1801 cgtcatttct tctaaagcca catcaggtac acgcgccatc gaatctcctt atgccgttct
     1861 ccgttttcga attaactatg ttcattttcc tgcggaactt ctgatttatc catccctggt
     1921 tagatttcgc tacatcggct cacgccccac cgcgctggtc acagagtctg tcggaacaat
     1981 ctgatcactt gaaccctcaa gctgctagaa gatccctatg ttcagcctcg ctgcgttcgc
     2041 gccctcccgt gttcaccatc attgccgggt ccatcgtaat cgattttcat cgtaaggacc
     2101 tccaacaatc agaaaaacta gccagttgat ttccagccgg cgcggctgaa agtgatcaag
     2161 aggtatggca tgaagcagcg gcgagtatct actttaactc gtttatccag tgagttctga
     2221 aagcaaccgc tgcaactcac gaaaatcgat tggcttgact aaatgagcat caaacccagc
     2281 ctctaatgcg cgttgtttgt cttcctcctg gccataccca cttgtgctaa ttagatgaat
     2341 ttccgacaag tcggaacaat ggcgtagccg tctagccacc tcgtagccgt cgattcctgg
     2401 caaacccaga tcgcagagca ccacttccgg ccgaaactgc tcgacaagag caatggcttg
     2461 agtgccattg tgggcaattt cagtctgatg cccaagttcg ttaagtaaca tttgggtgct
     2521 ccgggccgct aagaaattgt cttcgacaat gaccattctg aaaggacgaa ctttagctaa
     2581 atgcccatgc tcccgagaaa ccggtgagat actcggagca tcgagcggca gccgcaatgt
     2641 aaacgttgag cccttgccaa ctccctcgct ttcggccttc acttccccac cgtgcagctc
     2701 aaccaatcct tttaccagcg caaggccgag accgagtccg cctcggctac ggtcaagtgc
     2761 gcggtcggct tgagtgaacg tctcaaatat ccggggcagc gtggcggcat ctatgccgat
     2821 gccggtatcc cgtaccgtca caatcgcgct cttgctgtcg gaacttgcac cgaggactac
     2881 cgtcaccttg cccctgctct ccgtaaattt gctcgcgtta tgcagcagat tgccgatgac
     2941 ctgttcgatc cgcgttcgat caccggtaaa aaagaatggc tgatccggaa gtctgatctc
     3001 cagggacagc ccgctcgctt ttaaattttc gtggtaggca gtcacagtct ttgaaatgat
     3061 gcccgacagc tcgcaacgct ttttctccag gtgaatcttg cccgtcgtta tacgcgttac
     3121 atcgagcaag tcatcgatta aacgccgcat atgctgcacc tgcccgtcga tcatgtcacg
     3181 caattcgagc gacgcgtcag gcacgtctct gtagcgcata agctcaagcg cagttgtgat
     3241 gattcctagt ggatttctaa gttcatggcc tagcatcgcc aggaattcgt ctttgtgccg
     3301 gttagcttcc cgtaacgctg tttctgcttt cttgcggtcc gtgatgtcga ggttgatgcc
     3361 cgtgaaccgc atcggctgcc ctgtcgggtt ccgtaaaagt gtgccgactc ccatcaccca
     3421 cctttcgccg cgatccggat gtaaaatgcg gtactcgatt cgataatctg attccgagtt
     3481 aaaaacctca cgaccatatt gatcgactcg attacggtct tcgggatgta ccaaatggtt
     3541 tagccataag tcataagtga gcggaacatc cggggcaagt ccatacagct cccgatactc
     3601 cggcgacacg tagacaatgt ccgacgccag gtcccagtcc caaacgccgg cactcgctgc
     3661 gcgttgggcg agcgatagac tctcatttgt gcgcctcagc gcttcctcgg cattcgtgtc
     3721 aaggattgat ttatgatctg aagtatttcc gctcttacta atgtcgcgac cccgggaaga
     3781 taatcatcag gcatcttcat ttgctgaatt caacataaga tgcgcctctg caatataaaa
     3841 gaagggtgac ggatttctgg ggtccgacgg gaaccgatca accttgatgg tcgttggcaa
     3901 attcatcgca gaggggggtg aagtcggtgc atccaattcg ccaatgaact agcggacatc
     3961 ctcaagccgg cgccttgtcg taacgcatca gcccgttcat gacgacattc cagtccactg
     4021 agttagcctg tttccaattt cgttgtccgc cactccgaga cggatagttc gcggctggca
     4081 ccgaagttca tgcagatcaa gttctcagga gagtgtgatc ccctcttgcg cgcaggaccg
     4141 ggggcaccga tctttacctg agagactttt atgattctgc attaagggag cttttgagtg
     4201 atgcttgtcg ctgagttcgg ctacttccca tcaatccact ttttggcgag atccagagcg
     4261 aagttttcag cttcttcttt ggttcgaaag gcattattta gttttcagct tgaaagagct
     4321 tgggagcctt cttagtaata cggaactccg gcgtgtagcc gtttcggtcg aggctggccc
     4381 atgtggtaat cacaatactg tgagacttat aattgtgctc cattgaagta tttctcctgg
     4441 acgcgcttgc cggcgggccc tgcgccgtcc tgggttcgta ttacaactgt tctatcgtca
     4501 ttaaaaggct cggctaaaag ggaaatcgtg aattcctgcc ctgacggatg cgtcgtctga
     4561 tggtaagatc tttcggtgag cctaactcga tgctcttgtt gtggagatag attcaaccaa
     4621 cgaactatgg ttgccgtgac tctcggagca tctcaacata accatgttgt cgccgatgtc
     4681 aatgctggct ggcggccaac tctgcgatct tttttgattg gctgactcgc cacagcgtca
     4741 gtcttagctg ctccttgccg ttcgcttcac cacttcttta cacgccagag gagcacgata
     4801 gtaagtccaa agatcgcgaa ggcaagagct gcaatagcca attcagatag taaactcatc
     4861 gtgtaccgta ttgcctccta aatcgaataa aacgacttgc gtcgtttacg cgcataactc
     4921 atttcaaact gtttcgccga gcggcgaaat gggagggttt attatcacaa ccgaccttgg
     4981 tgtcaatcga gagggtgttt agaaccgcaa agaggcctcg atactgcgca gcccccacgg
     5041 tcctatgaaa cgtcaggcaa gcgagaacgc gaatggatcg ctccgcatca gcatttctct
     5101 aacctcgccg cgatgctgtt tctgctgttc agaaatctgt tgcagcaatt tgagtatgtc
     5161 gggaatatcg gattcgatac ctcgcagttg ttctgctaga tgatcggcgc ttcgattttc
     5221 atcatctaga gcgatcatca actgttgcca actgttttcg tcggttgaac gaagctcggg
     5281 tacgcgcggc agcttacctc caagtgcaac gatcgcctcg gcaatccggt ccgcatattt
     5341 ggttttttca tccgatatct ggaggagttt ttctcgaaac tggggataat gcatcttgtc
     5401 ggcgtgttgg ttaaatcggt cgcgctgctg cgtttcttcg acgtagcgct gctgcaagat
     5461 gttgatggct tgccggaagc tatcgtcagg caaatctaaa aatcggctcc accactccga
     5521 ccactgcttg attatgccca taaagttctc cctatttgga cactactgcg cccataaatg
     5581 agaaatcggt ctactattga acaatgg
//
LOCUS       DASUTG010000493         5528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_224373, whole genome shotgun sequence.
ACCESSION   DASUTG010000493 DASUTG010000000
VERSION     DASUTG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224373"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2604)
                     /locus_tag="VF089_11235"
     CDS             complement(<1..2604)
                     /locus_tag="VF089_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174578.1"
                     /translation="MLNLIKKIVGTKNDREVKRIRPYVEKINSFEAPLQSLSDDDLLA
                     KTDEFKSRIREATARLRTDLEEARAQAVNSEIEQREELKSRVEELDKELRQAEAEVLE
                     EILAEAFAAVREASRRTIGLRHFDVQMIGGTVLHEGKIAEMKTGEGKTLVATSALYLN
                     ALTGRGVHLITVNDYLARRDVQWMGPIFHKLGISVASIVHDASFRYDPTFLTKDYRFL
                     NLRPITRQEAYLADITYGTNNEFGFDYLRDNMKFSLDEYVQRELNYAVVDEVDNILID
                     EARTPLIISGPAEESTDKYYIIDRIIPKLQRGAVIQGDPTQEKRAAIEAQGDYTVDEK
                     SRSVTLTETGVAKVERLLGVHNLYDPRQIDTLHHVQQALKAHAIFKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKERVHIREENQTLATITIQNYFRMYKKLAGMT
                     GTADTEAPEFKKIYKLDVVVIPTHRNMVRKDLPDVVYRSDQEKYDAVVQEIEERYKKG
                     QPNLVGTTSVAKSERLSRMLKKKGIKHNVLNAVNHEAEATIIAQAGRYAAVTIATNMA
                     GRGTDILLGGNPEFLARSDMENEWINRAAKLPIQGAGRYEDALRELREKYEDEVRKAE
                     AQYLKQGELFQQQRSDALKRTTELQREIRETSPFRETRLRFEDLSATELIDVLHGGRA
                     IPNSYFQSKEELETALLQNFNGNKSEAYDAFEDARQEFSEYLQRWQQTAGERPALAEA
                     LDEKRRNYEQRVADFEFEVALAVLSGGEHGEIVNEYRTTYEDFETAELRCEEVRQPYE
                     EAIQQAQNDYELKRQEYLRMVEDIREQLDKAPDEYRQRFDEVLESYKKTCAEEREKVI
                     EAGGLHILGTER"
     gene            complement(2709..3509)
                     /gene="murI"
                     /locus_tag="VF089_11240"
     CDS             complement(2709..3509)
                     /gene="murI"
                     /locus_tag="VF089_11240"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX6174579.1"
                     /translation="MTNRDDAIGVFDSGIGGLTVLQKLIEILPSENTVYLGDTARAPY
                     GTKSVETVVRYSFENTEFLIEKGVKLVVVACNTSTALALEALRGAVNVPLIGVIDPGV
                     RKALLITKNRKVGVIGTEATIQSGAYTKALKTHARDLEVYSRACPLFVPLVEEGWIDN
                     DVASMTVEAYLGSLKQSGIDTLILGCTHYPLLKNAIRNFMGNAVQLVDSAEEIAREVG
                     TALENQSLARRDGTGNHSFFVTDAPDRFIKVGGRFLGEKVESAVRIER"
     gene            complement(3519..4586)
                     /locus_tag="VF089_11245"
     CDS             complement(3519..4586)
                     /locus_tag="VF089_11245"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6174580.1"
                     /translation="MIERDCQSTAMVNRLKTTLPHVPLKLVDHGRNEITFGTSASVDF
                     GAAKKRLYVAKHKGEFLKKCPGSDGQVCCNYFVINFASNCPMDCSYCYLQEYLARNPA
                     LKVFANIDDLVAEADELLTKHRRYFFRIGTGEITDSLILDPYIGFTRAVVPFFAQQSN
                     VLLELKTKSNCVEDLLTLDPRGRIVVSWSMNPQAVIDAEEHGTASLLERLQAARLCQD
                     AGYRLGFHFDPLIEYPDWQKDYEELVEQLFAMVDWKGISWLSMGVLRNTPGLKRAMRE
                     RFPGTRLLAAEQLPNPDGKLRYFQPLRIEMYRTMLGWIRRRAPTVKVYLCMESKHVWE
                     QVFGYAPSCEKELGAQLDPHV"
     gene            complement(4607..5182)
                     /locus_tag="VF089_11250"
     CDS             complement(4607..5182)
                     /locus_tag="VF089_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174581.1"
                     /translation="MTARAIQDYALSKGFHPQIRERWLSWNANDGAALLEVALSLKAG
                     ENHVRDLMDWLEEIALRDGIAVGEILRSKNISDIRTDPRLGRADRLKRIKDQIRRIRF
                     PRVSQIEDSIQSKIRQLKLPPTVRVSVPAGLEGGDLQITFRAGSPGEFKNILAKLGAA
                     AESDSLAEIYVLLNGAANAESDSALRSSSAC"
     gene            complement(5109..>5528)
                     /locus_tag="VF089_11255"
     CDS             complement(5109..>5528)
                     /locus_tag="VF089_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174582.1"
                     /translation="SAGGFDESFPVCEDYDLWLRIAVGHPVFLIDNPLVTKRGGHADQ
                     LSRSVWGMDRFRVTAIAKLLRAGLVGDKRQWALNSLRQKVSILAAGAAKRGRVEEARN
                     FETLLAEFLEGAGDDRTSHSGLCFEQRISSTDKGALA"
BASE COUNT         1197 a         1585 c         1348 g         1398 t
ORIGIN      
        1 acgctcggtc ccgagaatgt ggagcccgcc tgcctcgatc accttctcac gttcctctgc
       61 gcaagttttc ttgtagctct caagcacctc atcaaaccgt tgccgatatt catcgggagc
      121 cttatccagc tgctcgcgga tgtcctccac catgcgcaag tactcttgcc gctttaactc
      181 atagtcgttc tgcgcttgct ggatggcctc ttcatacggt tgccgaacct cttcgcagcg
      241 caattcggcg gtttcgaaat cctcataggt cgttctgtac tcgttaacga tttcgccatg
      301 ttcgcctcca gataacacag caagagcaac ttcgaattca aagtcggcaa cgcgctgttc
      361 ataatttcga cgtttctcgt ccagagcttc cgccaaagcc ggccgctccc cggctgtttg
      421 ctgccagcgt tggagatact cgctgaactc ctgtcgcgca tcttcaaaag catcgtaggc
      481 ttcggacttg tttccgttga agttttgcag aagcgcggtt tccagctctt ctttagactg
      541 gaagtagctg tttgggattg ctcttccacc gtggagcaca tcgataagct cggtagcgga
      601 aagatcttcg aagcgaaggc gcgtctcacg aaatggcgag gtttctcgga tctctctctg
      661 aagctcggtt gtacgcttca gcgcatcgct gcgctgttgc tgaaaaagtt caccctgttt
      721 taagtattgt gcttcggctt tccgaacctc atcttcatac ttctcgcgca actctcttaa
      781 cgcatcttcg taccgccctg ctccctgaat cggcaacttt gccgcacggt taatccactc
      841 attctccata tcggaacggg ccagaaactc cggattaccg ccaagcaata tgtcggtacc
      901 gcgaccagcc atgttagtcg caatcgtcac cgccgcatat cttcccgcct gagcaatgat
      961 cgttgcttcg gcttcgtgat tgacggcatt caggacattg tgcttgattc ccttcttctt
     1021 cagcatgcga gatagacgct cggacttcgc caccgatgtc gtcccgacga ggtttggttg
     1081 ccccttctta tatcgttctt ctatttcttg aacgaccgcg tcgtattttt cctggtctga
     1141 gcgatagacg acatccggaa gatctttgcg aaccatgttt cggtgggtcg gaatcaccac
     1201 cacatccaac ttgtaaatct tcttgaactc aggggcttcg gtatcggccg ttccagtcat
     1261 accggccaac ttcttgtaca tgcggaaata gttctgaatc gtgatcgtcg ccagcgtttg
     1321 gttttcttcc cgtatatgga cccgctcttt agcttccacg gcctggtgca aaccatcgga
     1381 ccagcgccgg ccaggcatta agcgtccagt gaactcgtca acaatgatga cttcgccgtc
     1441 tttgacaaca taatcaacat cgcgcttgaa aatcgcatgc gccttgagcg cctgttgcac
     1501 atggtgcaaa gtgtcgatct gccgcggatc ataaagatta tggactccca atagccgttc
     1561 gactttggcg accccggttt cggtcaaggt cacagaccga cttttctcgt cgaccgtgta
     1621 gtcgccttga gcttcgatag ccgcacgctt ttcctgggtt ggatcccctt gaatcacggc
     1681 gccgcgctgc aacttaggga taatccggtc gatgatgtag tatttgtccg tcgattcctc
     1741 tgctggaccg gagatgatca agggcgttct cgcctcgtcg attaaaatgt tgtcgacttc
     1801 atccacgacc gcgtagttta gctcgcgctg gacatattca tcgagggaaa acttcatatt
     1861 gtcgcgcagg tagtcgaaac cgaactcgtt attcgtgcca taggtaatat cggccaggta
     1921 agcttcctgc cgagtgatcg gacgtaaatt gagaaaacgg tagtctttgg ttaagaaggt
     1981 cggatcgtag cggaagctgg catcatgaac gatcgaagcc accgagatac caagcttgtg
     2041 gaagatcggc cccatccact gcacgtcgcg ccgcgccaaa taatcgttga ccgtgatgag
     2101 atgaacgccc cttccggtaa gagcgttcag atataacgcc gaagtcgcca ccagcgtctt
     2161 tccctcaccg gttttcattt cggctatttt gccttcgtga agcacggtgc cgccgatcat
     2221 ttgaacatcg aagtgccgca gcccgatcgt ccgccgcgac gcttcacgca ccgcggcaaa
     2281 cgcttcggct agaatttctt cgagaacctc cgcctcggcc tggcgcaact ccttgtccag
     2341 ctcttcaacg cgactcttga gctcttcgcg ctgttcgatc tcggaattga ctgcttgcgc
     2401 gcgagcttct tccaaatcgg tccttagccg cgctgtcgcc tcgcgtatac ggcttttgaa
     2461 ctcatcagtc ttagccagta gatcgtcatc gctaaggctt tggagtggcg cctcaaaact
     2521 attaatcttc tcgacataag gacgaatccg tttgacttcc cggtcgttct tagtgccgac
     2581 tatctttttg attaggttca acatgttaag actttgcccc caaagataac gcggagcggt
     2641 gcggcgcttg cgcaggaatt agttggctaa tagcttacaa gattcgggct aaaaacaaaa
     2701 tgaactcgtc agcgctcgat gcgcaccgcc gactccactt tctcgccgag aaatcgtccg
     2761 cccactttga tgaaccgatc gggtgcatct gtgacgaaga agctgtggtt accggtgccg
     2821 tctcttcgtg caagagactg gttttcgagt gccgtcccta cttccctggc gatttcttcc
     2881 gcggagtcga ccagctgaac cgcattcccc atgaagttcc ggatagcatt tttcagtaac
     2941 ggatagtgcg tgcatcccaa tataagagta tcgatgccgc tttgttttaa gctacccaga
     3001 taagcttcaa ccgtcatgga tgcgacatcg ttatcgatcc aaccctcttc gaccagagga
     3061 acgaacaagg gacaggcacg actatagact tcgagatctc tagcgtgcgt tttgagtgcc
     3121 ttggtgtagg caccgctttg aatcgtggct tccgttccaa tgacaccgac ctttctattc
     3181 ttggtgatca gaagcgcttt acgaacgccc gggtcgatca cgccgatcag aggaacgttt
     3241 actgctcccc gcaaggcttc caaagccaga gccgtcgaag tgttgcacgc aactactaca
     3301 agcttcacgc ccttttcaat caggaactcg gtgttttcga acgaataccg aaccaccgtt
     3361 tcaaccgact tcgtgccata gggagctctt gcggtgtcac cgagatatac ggtgttttca
     3421 gagggtagga tctcgattaa tttttgcaga actgtaagcc cgccaattcc tgagtcgaac
     3481 actcctatgg catcgtcgcg gttggtcatg acacagaatt atacatgcgg atcgagttgc
     3541 gcgcccagct ctttctcaca gctcggcgcg taaccgaaaa cctgttccca gacgtgtttc
     3601 gattccatgc acaggtagac tttgaccgtc ggcgcgcggc ggcggatcca gccgagcatc
     3661 gtgcggtaca tctcaatgcg cagcggttgg aagtagcgca gcttgccgtc aggattgggc
     3721 aactgctccg cggcaagaag acgagtcccc ggaaatctct ctctcatagc ccgctttagc
     3781 ccgggtgtat tgcgcaatac tcccatactc agccacgaaa tgcccttcca atcgaccatc
     3841 gcgaacagtt gctcgaccag ttcttcataa tccttttgcc aatccggata ctcgatcagc
     3901 ggatcgaaat gaaacccgag gcgataacca gcatcctggc agaggcgcgc cgcctgcaag
     3961 cgctcgagca aggatgcggt gccgtgctcc tcggcatcaa tcactgcttg tggattcatc
     4021 gaccacgaaa ccacgatccg ccccctggga tcgagtgtca atagatcctc aacgcagttg
     4081 ctcttggttt tgagctcgag caaaacgttc gattgttgag cgaaaaatgg tacaacagcg
     4141 cgcgtgaacc cgatatacgg atctagtatc aggctatcgg taatctcacc ggttccgata
     4201 cgaaaaaagt agcgccggtg cttggtcaat agctcgtccg cctctgccac taaatcatca
     4261 atgttggcaa atactttcag cgccggattg cgcgccagat actcttgcaa atagcagtag
     4321 ctgcaatcca taggacagtt actggcgaaa ttaatgacga agtagttgca gcagacctga
     4381 ccatcgctgc ctgggcattt ctttaaaaac tctcctttgt gtttggcaac gtaaaggcgc
     4441 ttctttgccg cgccgaagtc gactgaggcc gatgtgccga atgtgatttc gttgcggcca
     4501 tgatcaacga gtttcagtgg aacatgcggg agcgtagttt ttaaacggtt gaccatcgcc
     4561 gtgctttggc aatctcgctc gatcaaaatc tcctgtggct cgaatttcag catgctgatg
     4621 atgaccggag cgctgaatcc gattcggcat ttgccgcccc gtttaaaaga acgtagattt
     4681 cagccaagct gtcgctttct gccgccgcgc cgagctttgc cagaatattc ttaaattcac
     4741 caggactccc cgcacgaaat gtaatctgca gatccccacc ctcgagtccc gccggaaccg
     4801 ataccctgac agtaggcggc agctttagct gccgaatctt cgattgaatt gaatcctcga
     4861 tttggctcac acgcgggaag cgaattcgtc tgatctgatc cttgatgcgc ttcagacgat
     4921 cggctcggcc caagcgcggg tccgttctta tgtcgctgat gttcttactc cgcaaaattt
     4981 cgccaacggc gatgccgtct cgaagcgcga tctcttccaa ccagtccatc aggtcgcgta
     5041 catggttctc ccctgctttt aagctgagcg cgacctcaag aagcgcggcg ccatcgttcg
     5101 cgttccagct aagccaacgc tcccttatct gtggatgaaa tcctttgctc aaagcatagt
     5161 cctgaatggc tcgtgcggtc atcgccggct ccttctagaa attctgctag taacgtttcg
     5221 aaattgcggg cctcctcgac tctgcccctt tttgccgcac cggcagcgag gatcgatacc
     5281 ttctgccgaa gtgaattcaa ggcccattgt cttttatcgc caacaaggcc ggcgcgcagc
     5341 aacttggcga tggccgtcac tcggaatcgg tccatgcccc aaacagaacg ggataattga
     5401 tcagcatgtc cgcctcgctt ggtgaccaga gggttgtcga tcagaaaaac cggatggcca
     5461 acggcgatac ggagccacag atcataatcc tcacacaccg gaaatgattc gtcgaacccg
     5521 cctgccga
//
LOCUS       DASUTG010000494         7510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_76341, whole genome shotgun sequence.
ACCESSION   DASUTG010000494 DASUTG010000000
VERSION     DASUTG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76341"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VF089_11260"
     CDS             complement(<1..841)
                     /locus_tag="VF089_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174583.1"
                     /translation="MKNLLSDLYAGQTPSPITTKRVNWNKPIKLLTRYGLFFLAAAAI
                     LTISSPVRAASSPAKLVIAYAAMSPRVAPLWAAQERGFFNKNGINAELVFVRGAPTLL
                     AALSSGDMHFGLTGGTAVLGAAVAGFDLKILAVLTNRITFDLVARPGIRSAEELRGKR
                     FGTAAIGGTLWMSAILALERLGLDVNRDDIRFLAIGDAAVLTQALEDGRVDAALLDMV
                     FSRRLHDKGFPVLAELGKYDIPIASLGIVARSSYIQKNSQVTENLMRALLEGIAFVRA
                     PANK"
     gene            complement(1310..2452)
                     /locus_tag="VF089_11265"
     CDS             complement(1310..2452)
                     /locus_tag="VF089_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174584.1"
                     /translation="MARNLVIDADGHCYEPDSELAKWMPKELAHLAPNRVTDSSGYSH
                     LMIEGRLAARRRWGGGADRGQVFASHIERSRPGMTDPLKRLPDMDEEGIDVAIIFGTS
                     IALMVNGMADKPLAAALCHAVNRWLVEEYLAPDPKRLKGVGLIPLQDPAAAVKELEYL
                     AQQRSIVTAMLPTNVYGINLGHRMFDPVYAAAQEIGMPLSVHPQTEHDGQYGVWGVMG
                     AGSERMEKYSYVHMTAFPFELMIALMHLIGEGVFDRYPKLKIAFMEGACGWVPFWAER
                     MDEHFHKLRPQWPLLKREPSEIVKSSQVAFTCEPEETILPYVLDTIGITQVMYASDYR
                     HWDSEFPNSVHEVRKIPAMTDDKLRHVLGENARTWFNLKEADLPAR"
     gene            2780..3742
                     /locus_tag="VF089_11270"
     CDS             2780..3742
                     /locus_tag="VF089_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEX6174585.1"
                     /translation="METSFNTKPKLGISACLLGQKVRFDGGHKREQFLTDLFGRFVEW
                     VPICPEVEVGMGVPRETVRLVGTLANPRMIAEKSGRDWTSAMNHFAAKRLREIAELNL
                     SGYIFKKNSPTCGMERVRVYSSKGMPERQGRGLFAAAVMNQWPLLPVEEEGRLNDLKL
                     RENFIERVFAYHRWQRSSAERKSLGALVRFHTSHKYLLLAHSQRHYRQLGRLVATAKQ
                     KSLAALYDDYGRVFMEALAVHATEKTHANVLDHMMGYFSKQLTGSERQELVQLISDFR
                     HQLIPLIAPITLMRHYTRKYQVEYLQDQVYLEPNPKELMLRNHV"
     gene            4109..5338
                     /locus_tag="VF089_11275"
     CDS             4109..5338
                     /locus_tag="VF089_11275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174586.1"
                     /translation="MANTIFSAAYFQGFGVLILPIERTFGWDRWVISAAMSLRQLESG
                     IVSPAVGFLLDRFSARKLIFWSAVISGTGFIGLGFTNGIAMFFLCFVIISLGASGVSH
                     AVTWPVIISRWFRRNRGLATGLAVTGPIFGSPIVILNTQIEEAYGWRIVLFGYGILIL
                     VGITLLSMLVRDRPEPYGLRPDGDPPEDSAITEQHSSLRRRMDAGLTLHAVFRTKEFW
                     LFTSYLSGTFAVNSAVQGHMIPYFQQDIGLTAAWAAVVMSMVFIISGIGRIGGGYLLD
                     RMDYRLVLAVVAAMMGLGLLYLQVMPVKTVWATLPFAVMFGINFGCLVPMRGAVGSIM
                     FGTRTIGGILGLLQGGPIAAGVIGPLVMGLIFDLNGNYSTAIWGLIVISAFMIPLSLA
                     MGSPADLAKRIGASDQR"
     gene            complement(5495..6445)
                     /locus_tag="VF089_11280"
     CDS             complement(5495..6445)
                     /locus_tag="VF089_11280"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6174587.1"
                     /translation="MLSLHERFSGLFIPHVTPFDQSGAIDFESLNRLTAYFASLPGVA
                     GLVSCARIGESPVLSIEEKRQVYEVSGKVAREAGKVHIATIAPQSTDEAVALVSDLED
                     IPVDGVMIFPPLLFAWGKVDPDLKVRFFEDLARETKLPIVLFQIPVKSYYYDPATICR
                     IARLENIVAFKEASFDINLFRDTINALERDNAQMRVLTGNDRFVAESYKMGADGALIG
                     VANVATEKWGALDLAGRAGNFDQATAIQSELEAVKELVFSEPIVEAVARIKVVLQHEG
                     LIKATFVRRPQLGISQAEKKQLIDNYELLKNNKLRAAANQ"
     gene            6617..>7510
                     /locus_tag="VF089_11285"
     CDS             6617..>7510
                     /locus_tag="VF089_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6174588.1"
                     /translation="MNSAISCDALGRLMASEQRFAVFDVRERGEYNKCQIPNTTSLPR
                     SQIEFRIHELVPDREVQLIIYDEGGARAPLAATTLARLGYQNVALLNGGLSAWQSQGR
                     PTVSGVNVPSKAFGERIHHERNVPDLAPEELKSLQDRSTDLIILDVRTPEEYGRFCIP
                     GGINVPGGDLILWAGELKQRNATHVVVNCAGRTRSIIGAAALRRLGVSNVRALRNGTM
                     GWVLAGFELDKKPQRSSPPAPPRGPESAMALARHLAEEEAIAWISASELSRKLEAQSI
                     DYLIDVRSEGEFASGHVAGSIN"
BASE COUNT         1609 a         2057 c         2120 g         1724 t
ORIGIN      
        1 atttgttggc aggcgcgcgc acgaaagcga ttccctctag cagagccctc attagatttt
       61 cagtgacttg ggaatttttt tgaatgtaag aactgcgcgc aacgatgcct agactcgcga
      121 tcggaatgtc gtatttgcct aattccgcta agaccggaaa acctttgtcg tgcagccgcc
      181 ggctaaaaac catgtcgagg agggcggcgt ccacccttcc gtcctcgagc gcctgagtga
      241 gaacagccgc atcgccaatc gccagaaagc gaatgtcatc ccgattgaca tcgagtccca
      301 gccgttccaa agccaaaatg gcgctcatcc acaaagtccc accgatggcc gcggtgccga
      361 aacgtttacc gcgcagttcc tcggcgctcc taatgccggg gcgtgctacc agatcgaacg
      421 ttattcggtt cgtcaagaca gcaaggatct tcaaatcgaa cccggcaaca gctgccccca
      481 gaacagccgt tccacccgta aggccgaagt gcatatcgcc ggaactcaag gcggcgagaa
      541 gagtcggtgc tccgcggacg aaaaccagtt cggcattgat gccgttcttg ttgaaaaatc
      601 cgcgttcctg ggccgcccaa agcggtgcca cccttgggct catcgccgca taagcgatga
      661 cgagttttgc gggtgacgaa gcagcccgga cgggagagct aatcgtaaga atcgctgccg
      721 ccgccaagaa gaacaatccg tagcgagtca gcagcttaat cggtttgttc cagttcaccc
      781 gtttcgtagt tatgggtgat ggcgtctgac cggcgtacaa atccgataga agattcttca
      841 aagcgtacgc ctcgccgaca acattgctag cagttccttc ataagttctc cgctctcgta
      901 actcgttgga gtcgtccaag tcaaccttta atgttgttgc aaactgttca aagatgatcg
      961 agtacagtcg cgagcgtgat gatggtagag tggagtgccg gcggccgtaa gaaggttgta
     1021 acaattagat cactttttga tggttccata aatatccgca tgcatattga tcgcattcga
     1081 taattccgac gtctgcggcc gagaactgca aatatctgaa ggtggattac cgccagccag
     1141 gtgctagaag tgcttacgga caggatgaca gcgaaagtga tcctgaggaa ttatcgtatg
     1201 tcgaacagcg atatcgttgc ctagagatac ggatattcgg cagcccggca aagtaggatg
     1261 cctagatgaa gaaggtctac tctggcgtgg cggtttgatt ctggatcgat tatctcgccg
     1321 gtaaatccgc ttccttcagg ttgaaccaag tgcgtgcgtt ctcgccgagt acgtggcgca
     1381 gcttgtcatc ggtcatcgcg gggatttttc tgacttcatg caccgagttg ggaaattccg
     1441 aatcccagtg gcggtagtcg gacgcgtaca tcacttgggt gatgccgatg gtatcgagaa
     1501 cgtaagggag aatggtttct tcgggctcac aggtaaaagc aacctgggag ctttttacta
     1561 tttcggaagg ctcgcgtttc aaaagcggcc attgcgggcg cagcttgtga aagtgctcat
     1621 ccatgcgctc cgcccagaac ggcacccagc cgcaggcgcc ttccatgaag gcaattttaa
     1681 gtttgggata acgatcgaaa acgccttcgc cgatcaagtg catgagcgcg atcatcagct
     1741 cgaatggaaa cgcggtcatg tgcacgtaac tgtatttctc catgcgctcg ctgccggcgc
     1801 ccatcactcc ccagacgccg tattgtccgt cgtgctcggt ttgcggatgg accgacagcg
     1861 gcatgccaat ctcttgtgcg gcggcgtaaa cgggatcgaa catgcggtgc cccaaattga
     1921 tgccgtaaac gttcgttggc agcatggccg tgacgatcga gcgctgctgc gcgagatatt
     1981 ccaattcttt cacggccgcg gccggatctt gcaacggaat cagtccgacg cccttcaagc
     2041 gttttggatc gggcgcgagg tattcctcga ctaaccatcg attgaccgcg tgacacagcg
     2101 cggccgccag gggtttgtcg gccatgccat tgaccatgag cgcgatcgag gtgccgaaga
     2161 tgatcgcgac atcgatccct tcttcgtcca tgtcgggcag gcgcttcaac ggatcggtca
     2221 tgccgggacg gctgcgctcg atatgcgatg cgaacacttg accgcgatcc gctccgccgc
     2281 cccagcggcg gcgcgcggcg aggcggcctt cgatcatcaa gtgtgaatag cctgagctgt
     2341 cggtcacacg attgggcgcc agatgagcaa gctctttggg catccatttc gcaagctcgc
     2401 tatccggctc atagcagtgg ccatcggcat cgatgactaa atttctcgcc ataattcctc
     2461 cacgccgtct tcgagatttg ctatctttcc tagtcggctt ctcccgcgaa tgcaactgtt
     2521 tccggccact cggcctcggc gtgacgatcg gccaataata cgtgcaacgc atgatgctgg
     2581 agcaaaaagt gtattgaaag tacgaaggat atacagaacc gacatcgaag ctagaacgcg
     2641 agcccgcttt aaacaggttc tcttccctcg tgcgcttcgt gtcctccctg gtgtactccg
     2701 acttagagtt tctgatctgc gttgaattgg cgaaaagttg actcccgtcg ccgtaatgct
     2761 atcaacagtc aacacgggaa tggaaacaag tttcaatacc aagccaaaac tcggtatcag
     2821 cgcctgcctg ctcgggcaga aggtccgctt cgacggcggc cacaagcgcg agcagtttct
     2881 gaccgatctg tttggcagat ttgtcgagtg ggttccgatc tgtccggagg tcgaagtcgg
     2941 catgggtgtg ccgcgcgaga ccgttcgcct ggtgggcacg ctggcgaatc ccagaatgat
     3001 cgcggaaaaa tcaggcagag actggacctc ggcgatgaat cacttcgcgg cgaagcggct
     3061 gcgggaaatc gctgagctaa atttgtcggg ctacatcttc aagaagaatt cacctacctg
     3121 tggcatggag cgtgtaagag tttatagctc caaaggtatg ccggagcggc aaggccgtgg
     3181 tcttttcgcc gcggcggtca tgaaccaatg gccgcttttg ccggtcgaag aagaaggccg
     3241 gctgaacgat ctcaagctca gagaaaattt catcgaaaga gttttcgcct atcaccgctg
     3301 gcaacgatca tcggccgagc gaaaatcgct cggggcgctg gtgcgctttc acacgagcca
     3361 caaatatttg ttactcgccc acagccaacg tcactatcgc caactgggca ggctggtggc
     3421 gacggcgaaa caaaaatctc ttgccgcgct ttacgacgac tatgggcggg ttttcatgga
     3481 ggctttagcg gttcacgcta ctgaaaagac ccatgccaat gtcttggacc atatgatggg
     3541 ctatttctcc aagcaactga ccggatcgga gagacaagaa ctggtgcagt taatcagcga
     3601 tttccgccac cagctcatcc ctttgattgc gccgatcacg ttgatgcggc actacaccag
     3661 gaaatatcag gtcgagtact tgcaggatca agtttattta gaaccaaacc cgaaagagct
     3721 gatgctgcga aatcatgtct agcattagac ttcccgtgcc atgaatgatt tggcttgacg
     3781 aggatcattg aatacgaacg cctgacttct ctacttgtgg ctcaaagaag cctagcctgc
     3841 attctacaag tcgtgccgag cctttaactc cgtagcgtgt tatatatcgg tggatgtgtt
     3901 gcgcccgacc agccgggcgg tgtcatgttt aatccacttt gattgaaacc cttttcagtg
     3961 gactgtctca acagagccgc gatgagaacg attatgatat tcccgcgtgc ctaaactcga
     4021 gcccatttga tcgacatcca tgccacttga aaagtctgga aagcggcagc cgatcttttt
     4081 cggctggtat atggttgccg caagtgtcgt ggccaacacg atcttctctg cggcgtattt
     4141 ccagggcttc ggcgttctca ttctccccat agagcgcacc ttcggctggg accgctgggt
     4201 tatttccgcc gccatgtcgc tgcgccagtt ggagtccggc attgtcagtc ccgctgtcgg
     4261 cttcctcctg gatcgcttta gcgcgcgcaa gttgattttc tggagcgcgg tcatatcagg
     4321 cacgggcttt atcggcctcg gcttcaccaa cggcatcgcc atgttttttc tctgcttcgt
     4381 gatcatttcc ctgggagcat ccggcgtcag ccacgcggtg acctggccgg tgatcatctc
     4441 gcggtggttc cgtcgcaacc gcggcctggc caccggcctt gcggtaaccg gtccgatctt
     4501 cggctcgccc atcgttatac ttaacacgca gatcgaggag gcctatggct ggcgcatcgt
     4561 gctgttcggc tacggaatct tgatcttggt cggcatcact ttgctcagca tgttggtgcg
     4621 cgatcggccc gagccctatg gcttgcgccc ggacggcgat ccgccggagg atagtgccat
     4681 cacagaacag cattccagct tgcgccgccg aatggatgcg gggctgacgc tgcacgccgt
     4741 gttccgcacc aaagagttct ggttatttac gagctacttg tcaggtacgt ttgcggtcaa
     4801 ttccgccgtt caggggcaca tgattccgta cttccagcaa gacatcggac ttaccgccgc
     4861 ctgggcggcc gtggtcatgt ccatggtctt catcatcagc ggtatcgggc ggatcggcgg
     4921 cggttacctg ctggacagga tggactatcg actcgttctc gcagtcgtcg ctgcgatgat
     4981 gggtctcggc ctgttgtatc ttcaggtgat gccggtcaaa acggtttggg ccacattgcc
     5041 attcgcggtg atgttcggca tcaacttcgg ctgcctcgtt cccatgcggg gtgccgtcgg
     5101 cagcatcatg ttcggcaccc gaaccatcgg cggaattttg ggcctgctgc aaggcggacc
     5161 gattgcggcg ggggtcatcg gccccctagt catggggctg attttcgatc tgaacgggaa
     5221 ctactccacc gccatctggg gactcatcgt cataagcgct ttcatgatac cgttatccct
     5281 ggcgatgggc tcgcccgccg atttggccaa gcgcattggc gcatctgatc aacgttaggc
     5341 gcgctgacct gagtcttatc caggtcaaaa cctatgatgg tcgtgacagg tggtcgctgc
     5401 ttctaacggc acgtcaattc aattctgtcg tccgctagca gcgcagaagc gggatccctt
     5461 gcttcttctc gggaagacac ggagcacggg tgtgctactg gttggcggcg gcgcggagtt
     5521 tgttgttctt tagcaactcg tagttgtcga tgagctgttt cttctcggcc tgtgaaatgc
     5581 cgagctgggg gcggcgcacg aaggttgctt taatcagccc ttcatgttgt aacacgacct
     5641 tgatccgcgc cacggcttcg acgattggtt cgctaaagac gagctctttc acggcttcta
     5701 attcactttg gatggcggtg gcttgatcga agtttccggc gcgtccggcc aaatccaacg
     5761 cgccccattt ttccgtcgca acattggcca cgccgatgag cgctccgtca gctcccattt
     5821 tataagactc cgctacgaag cggtcgttgc cggtcaagac tcgcatttgg gcgttgtcgc
     5881 gttccagcgc gtttattgtg tcgcggaaaa gattgatgtc gaaagacgct tctttgaagg
     5941 cgacgatgtt ttcgagccgg gcgatgcgac agatggttgc cggatcgtag tagtagctct
     6001 taaccggtat ctgaaagagc acaatgggca gcttggtttc tcgggccaga tcctcgaaga
     6061 agcggacttt gagatccggg tcgaccttgc cccaggcaaa cagcaacggc ggaaaaatca
     6121 tcacgccgtc gaccgggata tcctccagat cgctcacaag cgcgactgct tcatcggtag
     6181 actgtggcgc gattgtagca atatgcacct tgccggcttc acgagcgacc ttgccggaaa
     6241 cttcataaac ctggcgcttc tcctcgatgc tcagcaccgg gctttcaccg atacgcgcgc
     6301 acgaaaccag tccggcaacg ccgggaagcg aagcgaaata ggcagtcagc cgattcaagc
     6361 tctcaaaatc gatggctccg gattgatcga acggcgttac atgggggatg aagaggccgc
     6421 tgaaacgttc atgaagggat agcataagtc ctccgggctg gttgtgctaa gaactcgata
     6481 gacaatatcg tgcttgaaaa agactcacgc ataagtagaa cagattggcc ggataggtca
     6541 aggcaaacga tctttcagtt catgccaaac taagactgct gtccggctcc ttcataggga
     6601 tcgagggagt agaaaaatga actcggctat ttcgtgcgat gcccttggcc ggctgatggc
     6661 atcggagcag cgtttcgccg tttttgacgt gcgcgaacgc ggtgaataca acaaatgtca
     6721 gattcccaac acaacatcac tgccgcggag ccagatcgaa tttcgtatcc acgagttggt
     6781 gcccgatcgt gaggtgcagc tgattattta cgacgagggc ggtgcgcgcg caccattggc
     6841 cgcgacgacg ttggcgcgcc tcggctatca aaatgtcgcg ctactgaatg gcggcttgag
     6901 cgcatggcaa agccaaggac ggcccacagt gagcggcgtc aatgtcccaa gcaaagcttt
     6961 tggcgagcga atccatcacg agcgcaacgt tccggattta gctccggaag agcttaagtc
     7021 gctgcaggat cgttcgacag atctgatcat tctcgatgtt cgcactcctg aggagtacgg
     7081 ccggttttgc attccgggcg gcatcaacgt tcccggcggt gatctgattc tctgggccgg
     7141 agaattgaag cagcgtaatg cgacacacgt cgtggtgaat tgcgccgggc gcacgcgcag
     7201 tatcattggc gccgcagcgc tgcgccgcct cggggtaagc aacgtccggg cgctgagaaa
     7261 tggaaccatg ggatgggtgc tcgctggttt cgagttggac aaaaagcctc aacggtcctc
     7321 gccccctgca cctcccagag gtcctgaatc ggcaatggcg ttggcacgcc acttagccga
     7381 ggaagaagcg atcgcgtgga tctctgcttc agagctgtcg cgtaagctcg aagctcaaag
     7441 catcgattac ctgattgacg ttcgctccga aggcgagttc gcatccggcc atgtggccgg
     7501 ctcaatcaat
//
LOCUS       DASUTG010000495         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2002833, whole genome shotgun sequence.
ACCESSION   DASUTG010000495 DASUTG010000000
VERSION     DASUTG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002833"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..893)
                     /locus_tag="VF089_11290"
     CDS             complement(105..893)
                     /locus_tag="VF089_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6174589.1"
                     /translation="MIRSASELKGYTIEANDGDIGEVVQFYFDDEKWTIRYLVADTGG
                     WLMGRRVLISPAALGRVDWNSRRLRVNMTKERVENSPSIDTDRPVSRQHETDYYNYYG
                     YPYYWSGPYVWGPMPYPAYPPVAPAGTASPAGAPGTVEREAAQAQLQDEDVHLRSTQE
                     VTNYYIEASDGDIGHVEDFLLDDESWTIRYVVVDTRNWWPGKKVLVSPEWIRSVSWSD
                     SRVYVELTRDKIKNAPEYDPSLPLSRDYETRLHKHYGRSEYWNA"
     gene            1243..1956
                     /locus_tag="VF089_11295"
     CDS             1243..1956
                     /locus_tag="VF089_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174590.1"
                     /translation="MMITLEIYQHVLLARVVAAITVLWFSVFVAGCASNLKPAAQAQI
                     VRNNEPAAFSEVAGIRVAVVPNQWSGSPADLGDLVTIFRVEIENGSDQPLRIGYREFK
                     LVGPGSFTNTAIPPYQVEGTVVKPVFLPQTYVYPHGFQLAPHYRTRIEDTSRPRHPWD
                     YDDYTPRYNLWASPLPTEDMLAKGIPEGILEPKGRISGFLYFAKLPENLDRVTFEADL
                     VSVRTGVTIGTVRIPFVIE"
     gene            2105..3004
                     /locus_tag="VF089_11300"
     CDS             2105..3004
                     /locus_tag="VF089_11300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6174591.1"
                     /translation="MLHAFALIPASTKAGSSFMINRHTVNELVHRIWRNVVWHDCFGK
                     AAELGFYFLLALFPLLIFLLSLISFMRGAEEIIISWLRTLMPQDSMGMINKWIKNFLS
                     RRSGGLLSFSLIFSLWSASQGVRALLVALNRAYEVEEGRPFWKSELLALAITLALCLL
                     VIGGVVLITFGDPLLRSFRNLLGLEIGAGSLWLILNYTTGMAMLILGMGFVYFFGPNV
                     QQDTRQIAPGTLFAVTAFIIVSYLFSLYLRYAPSYQAVYGSLGAVIILMLWLYLMAFI
                     MYLGGEINAEVQKLAGKPVEPKQ"
BASE COUNT          758 a          905 c          876 g          828 t
ORIGIN      
        1 cagatcgcct gcacagcagg gatcgactgg acgctgagtt gcctcatctc gcttgcgccg
       61 cctccaccgc cttaaaggtc cctcgttcga ttagctcacg gtcatcatgc attccagtat
      121 tcagatcggc cgtaatgctt gtgcagccgt gtttcataat cgcggctcag cggcaatgac
      181 ggatcatact caggagcgtt cttaatctta tctcgtgtca gctcgacata aactcgcgaa
      241 tcgctccagc tcaccgagcg aatccattcc ggagagacca ggaccttctt tcccggccac
      301 cagttacgtg tatcgacgac aacataccga atcgtccagc tttcatcgtc aagcaagaag
      361 tcttcaacat ggccgatgtc accatcgctg gcttcgatgt agtagttggt tacctcctgc
      421 gtgctgcgca agtgaacatc ctcgtcctgc agttgggctt gtgccgcctc gcgctcgacg
      481 gtgccaggcg ctccagcggg actagcggta cccgcaggag caaccggtgg gtaggcgggg
      541 tatggcatcg ggccccagac gtagggtccg ctccagtagt aggggtatcc atagtagttg
      601 tagtaatccg tttcatgctg tcgggaaacc ggccgatccg tatcgatgct gggactgttc
      661 tcgacccgct ccttggtcat gttcacgcgc agcctacgtg agttccaatc cactctgccg
      721 agagccgccg gcgagattaa aactcttctc cccatcagcc acccgcctgt gtcggccacc
      781 aggtacctga tcgtccattt ctcatcatcg aaatagaact gcaccacttc gcctatgtct
      841 ccatcgtttg cctcaatcgt atagcccttg agctcactgg cgcttctaat catgagatgg
      901 ccctcctttt tggttaaacc tctttcactt atccacagca agcgcgatgc cattttagcg
      961 tcgattactc tcgaattatt ggcgctcagg gtgagaacac tgcgtcggat tcgcgacact
     1021 ttaactggcg tgatgattat gcctcgccgc cgaaggctcg tactcgtttg tagtcgtctt
     1081 aagaaacgtt tgatgtcggc ccggtagaac gggggaaact ggttgatgct ggcacgctga
     1141 ttgcgctttt ttctctcgat agccccttca caatggcggt ccttggcacc ggtaagtaga
     1201 agagtagatc agcggctcct gcgtgaagca gattcaggaa ggatgatgat cacattagag
     1261 atttaccagc acgtgttgct cgccagagtc gttgccgcaa tcaccgttct ctggttttct
     1321 gttttcgtgg ccggatgtgc gtcgaatctc aaaccagcag cgcaagcgca aatagttcga
     1381 aacaacgagc ctgccgcatt cagcgaagta gccggtatcc gggtagcggt tgtgccgaat
     1441 caatggtccg gcagtccggc agatctgggt gacctcgtga cgatcttccg ggtggagata
     1501 gaaaacggca gcgatcagcc attgcgcatt ggttatcgcg agttcaagct cgttggtccg
     1561 ggatctttta caaatacggc catcccgccg taccaggtag aaggcacagt ggtaaaaccg
     1621 gttttcttgc cacagacgta tgtttaccct catggttttc aattggcgcc gcattatcga
     1681 actcggattg aggacacaag ccggccgcgg catccctggg attatgatga ctatactccc
     1741 cgctacaatc tgtgggcatc cccgctgcca acagaagaca tgctggcgaa agggattccg
     1801 gaaggcatac ttgaaccgaa ggggcgcata tcaggatttt tatattttgc gaagctcccg
     1861 gaaaatctcg accgggtaac tttcgaggcc gaccttgtaa gcgtccggac cggggttaca
     1921 atcggaacgg tgcgcattcc gttcgtgatc gagtgatcac tatgcactcc cacgacctaa
     1981 tcaagtcgca gcaacacaga atctccgcag ctgcgccaaa cggctccgtg aaaacatgct
     2041 ggattgaagg cgggttcttt cctgagaaag acagaataat tcttgaacca gaggagctaa
     2101 acgtttgctt catgccttcg cgctgattcc cgcttcaact aaggccggca gcagcttcat
     2161 gatcaatcgc catacggtca acgaacttgt tcacaggatc tggcgcaacg tggtttggca
     2221 tgactgcttc ggcaaagcag ccgaacttgg cttctatttc ctactggcac tatttccgct
     2281 gctcatattc ctgctcagtc taatcagttt catgcgcggc gccgaggaaa ttatcatttc
     2341 ctggctaaga actctgatgc cgcaagattc tatgggtatg atcaacaaat ggataaagaa
     2401 ttttttgagc aggcggagcg gaggactgct gtcattcagc ttgatcttct ctctttggtc
     2461 ggcatctcag ggtgtcagag cgctgctggt cgcgctgaac cgagcctatg aagtggagga
     2521 agggcggccg ttttggaagt ctgagcttct ggcgcttgca atcaccttag ctctttgcct
     2581 tctggtgata ggcggcgtgg ttctcatcac ctttggtgac ccgctgctga gatcgtttcg
     2641 taatttgctg ggtctggaaa taggcgcagg ctcgctatgg cttatcctca actacacaac
     2701 cggtatggcc atgctcatcc ttggcatggg tttcgtctac tttttcggac ccaacgttca
     2761 acaagacacc agacaaatcg cgccgggtac gctatttgcc gtaacggcgt ttataatcgt
     2821 ctcgtatctt ttctccctgt atttgagata tgcgccaagt taccaggcgg tctatggcag
     2881 ccttggagcc gttatcatcc tgatgctgtg gctatacctg atggcgttta ttatgtatct
     2941 cggcggagag attaatgcgg aagttcaaaa actcgccgga aagccggtcg agccaaagca
     3001 gtgaatccgt gattttcttc gaaagccgtg accacactta caccggtcca gtaaccgatt
     3061 gtgctgcaac acgagtcaca cggcttcctg attgccacgt aatcgtatcg gaggcgcaac
     3121 cttcaaagaa ataaagcgca acccaaatcg aaattgctat cgctcggtct aagaaactca
     3181 gcacagagtg aacctaagaa ggaggtaaag agatggagcc atcaagaatc agagcgattg
     3241 acctgctgga tcggatggag gctggagaag aaaccgtatt gatcgatacc aggaacccca
     3301 aggcctgggc ccaatccgat cagaagctgc cgggagcttg cgaattcctg cgaacgaaat
     3361 cgaggct
//
LOCUS       DASUTG010000496         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1856743, whole genome shotgun sequence.
ACCESSION   DASUTG010000496 DASUTG010000000
VERSION     DASUTG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856743"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF089_11305"
     CDS             complement(<1..217)
                     /locus_tag="VF089_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174592.1"
                     /translation="MALSESLNDHERNSRIEWRLYYNMLVTRRFEEALLRWEHQGKIA
                     AQTFPSKGQEAVAIGCCLALERGDVVIP"
     gene            complement(299..1411)
                     /locus_tag="VF089_11310"
     CDS             complement(299..1411)
                     /locus_tag="VF089_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174593.1"
                     /translation="MIIVKDLQKSFAGARGEVRALRGVSFEVGKGELFTLLGPSGCGK
                     TTTLRCIAGLEQPLAGEIVIDDHPVFSDGGIFIPPERRNIGMVFQSYAIWPHMTVFQN
                     VAYPLARDVSRAEIGERVGRILDRLSLGQLSDRLAPNLSGGQQQRVALARALVAQPQV
                     LLLDEPLSNLDAKLREQMRFELKALQESFGITTVYVTHDQEEALALSDRIGLMHEGAL
                     IEIGSPADLYLRPAHRVTADFLGTANFIPAEVQRDGGGPWDGMLLATPLGSFGAQRSV
                     EWHQGMTAQLFFRPESVEFLDEAVAAADNARNHCTGFIERVTFLGSSADVIIRCSDLK
                     VRARTHPARAPAVGQAVRFRVAQSSCIVFPSRTMNS"
     gene            complement(1519..3297)
                     /locus_tag="VF089_11315"
     CDS             complement(1519..3297)
                     /locus_tag="VF089_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6174594.1"
                     /translation="MSVAQPTLDSGVRKQALSWTRKFSVRAVTLGFTIIVVSYLVLVP
                     LIMLLYASVKSTEDKLPFETTGMTLANYITVLTSSSTLPIFLNTFYFTAGSLAIGLPL
                     AVLMAWLLERTAIPARRWIAALILVPMTIPSLLSAIAWIQLLDPRIGLVNVVLRNLLG
                     LTGDSGPFDIYTLSGMCFVQGLRLVPSAYLMIAASFRAMDPSLEEQSAMAGRGVGHTT
                     LRITLPIMRPALLAMLIYYIIVVIESFDIPGLLGFTARIRVLSTAIYWATHSEVGLPD
                     YGLASALGALVLAMALLLMWAYQRLTAHQERFTTITGKGYRPRQINLGGWTAPATALC
                     VVYVALAVVLPFLMLIWTSLQPFYAVPSADSLARVTLDGYVSIWQESTITRALWNTTV
                     LALVTSAATILLALLVSWFVVRRTRVADALSHYLATVSFLPQCVPSIVIGLAFIFVYV
                     RFPIPIYGTLWIIALAMTTRYLAYSSRAMTSALMQVHGELEEASQMSRAPWARTMRKI
                     TMPLLAPAMINVFFWVAVHAMQELSMALMLYNPDTVVVSTMIWSMWQNGRTTDAAALG
                     VILILLSAVLLLGGQLFARFRQGANP"
     gene            complement(3465..4526)
                     /locus_tag="VF089_11320"
     CDS             complement(3465..4526)
                     /locus_tag="VF089_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174595.1"
                     /translation="MKSRAIVAAVMICLMSVFFSDMGCAQEKGKGDSDGIRELYHAAR
                     KEGRVVIWGPTDAIVYQRMQEALDKQYPGIKIESFESIPEPLVQRIIAESQAGKPAAV
                     DVIQSGSLRALRPLIDRDMLAPYSGWEKIFGLDAVYADQRLVGGYNLTLPIAYNTKTV
                     SAQEAPKRWEDLADPKWKGRKIIIEARLVPFAMLGTEWGKDKAIELTKKIVSQQQPLI
                     VQGGTTVANALAGGQVSIAVGTYAFTIEGLKKQGAAVDWVPVSPLPVLTSAEGVLKTA
                     PRPNAGRFFAGWMGTPEGQKIRYATRGQAMEVGKNAIGNVAERIRNEKPPVILETDKT
                     FTSILEIQREMGKLRGALR"
     gene            complement(4566..>4824)
                     /locus_tag="VF089_11325"
     CDS             complement(4566..>4824)
                     /locus_tag="VF089_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174596.1"
                     /translation="KMPTANLNDVQIFYEDSGESEGAEDDANRGGSTPVATARRLAEL
                     VPGSELALVPNVAYDILGRWRRADCVKKFFAAPSDSLAIER"
BASE COUNT         1083 a         1461 c         1305 g          975 t
ORIGIN      
        1 acggtatcac cacgtctcct ctctccaacg ccaggcagca accgatggca accgcttcct
       61 gccccttgct cggaaaggtt tgcgccgcaa tctttccttg atgctcccag cggagcaaag
      121 cttcttcgaa acgccgcgtc acgagcatgt tatagtagag gcgccactcg atgcggctat
      181 ttcgctcatg atcgttgagc gattccgaca atgccaccac tccctcctaa atatcgattg
      241 atgatggaac ggcttgacat cctcaccttg cttctcccac tcagtgggcg aagtaattct
      301 agctattcat agttcgactc ggaaaaacga tacacgagct ctgcgctacg cgaaaacgca
      361 cggcttgccc cactgccgga gcgcgcgcag gatgagttct cgcgcggact ttgaggtcgc
      421 tacatcgaat gatcacgtct gctgaactgc cgagaaatgt gacacgctcg atgaagcccg
      481 tgcagtgatt ccgcgcatta tccgcagcag caactgcttc atcgagaaac tcgacggatt
      541 ccggacgaaa gaacaactgc gccgtcattc cctggtgcca ttccacgctg cgctgcgcgc
      601 cgaatgagcc aagcggcgtc gctaaaagca ttccatccca cggtccgccg ccatcgcgct
      661 ggacttctgc tgggataaaa ttcgctgtgc ccagaaaatc cgccgtaact cgatgcgcgg
      721 ggcgcaaata aagatcggcc ggcgaaccga tctcgatcag cgcgccttca tgcatgaggc
      781 cgatgcgatc cgatagcgcg agcgcctcct cctgatcatg ggttacatac accgttgtga
      841 tgccgaacga ctcctgcagt gctttaagct cgaaccgcat ctgctcgcgc agtttggcgt
      901 cgagattgct gagcggctcg tccaggagca gcacctgcgg ctgcgccacc agcgcgcgcg
      961 ccagcgccac ccgctgctgc tgaccgccac tcaagttggg cgcgagccga tccgacagct
     1021 gaccaaggct caaccggtcg agtatcctgc cgactcgttc gccaatctca gcgcggctca
     1081 cgtctctggc cagcggatag gcgacattct gaaagacggt catgtgcggc cagatcgcat
     1141 aggattgaaa aaccatgccg atattgcgcc gctccggcgg aataaagata ccgccgtcgc
     1201 tgaaaaccgg atgatcgtcg atcacaattt cgcccgcgag gggctgctcc aatccggcaa
     1261 tacagcgaag cgtcgtcgtc ttgccgcagc cgctagggcc gagcaaggta aacaactcgc
     1321 ctttgcccac ttcaaaggag acgccgcgga gcgcgcggac ttcgcctctg gcgccggcga
     1381 aagatttttg cagatcttta actatgatca tttcgaaacc gacttattcc gaagctttgc
     1441 gctcttggcg gcttggcgcg agtgaaattc cggattctcc cgcaatgccg gcaagctcgc
     1501 caagaatcaa gggctggcct agggatttgc accctgccgg aagcgcgcaa acagctgtcc
     1561 gccgagcagt aacacggcgg ataagaggat caaaattacg cctagagccg cggcatcggt
     1621 ggtccgcccg ttttgccaca tgctccagat catcgtcgaa accactacgg tatccggatt
     1681 gtagagcatg agcgccatcg acagctcctg catggcatgg accgccaccc aaaaaaagac
     1741 gttgatcatg gccggtgcca agagcggcat ggtgattttg cgcatggtcc gcgcccacgg
     1801 cgcgcgtgac atctgtgatg cttcttccag ctcgccatgc acctgcatga gcgcggaagt
     1861 catcgcgcgc gagctatacg cgagatatcg cgtcgtcatc gccagcgcga tgatccacaa
     1921 ggtgccgtag attggaattg gaaatcggac gtagacaaag ataaatgcca gtccgatgac
     1981 gatgctcggc acgcactgcg gcaagaatga caccgtcgcc agataatgcg aaagggcgtc
     2041 ggcaactctc gtgcgccgca cgacgaacca gcttaccaga agggcgagca gaatcgtcgc
     2101 ggcggaggtc accagcgcca gtaccgtcgt attccacagc gcgcgagtga tggtgctctc
     2161 ctgccaaatg ctcacgtatc cgtcgagagt gacgcgcgcg agggaatccg ccgaagggac
     2221 cgcatagaac ggctggaggc tcgtccagat gagcatcaga aacggcagca ctacggccag
     2281 cgcgacatag acgacacaaa gcgcggttgc cggcgccgtc catccgccaa gattaatctg
     2341 ccgcgggcga tagcccttgc cggtgatcgt ggtgaatctt tcctggtgcg ccgtcaaccg
     2401 ctggtaggcc cacatgagca gtagcgccat ggctaacacc agcgccccca aagccgaagc
     2461 caaaccataa tccggcaagc cgacttccga gtgggtcgcc caatagatcg ccgtgctgag
     2521 aacgcgaatg cgcgcggtga aaccgagcag cccgggaatg tcaaaggatt cgatgaccac
     2581 gataatgtag tagatcaaca tcgccagcag cgcgggcctc atgatcggca gagtgatacg
     2641 caatgtagtg tggccgacac cgcgacccgc catggcgctc tgttcctcca gtgatggatc
     2701 catggcgcga aacgaagccg cgatcatgag atacgccgac ggcactaggc gcaagccttg
     2761 aacgaagcac atgcccgaga gcgtgtagat atcgaacggt ccggagtctc ccgttaagcc
     2821 gagcaggttg cgcaagacaa cgtttaccag accgatgcgc ggatcgagca gctgaatcca
     2881 ggcgatcgcc gagagcagcg acggaatcgt catcggcacg agaatcagag cggcgatcca
     2941 ccggcgggcg gggattgccg tgcgctctaa aagccacgcc atgagaacgg ccagcggcag
     3001 gccaatcgcc aatgatccgg ccgtgaagta aaatgtattc agaaaaatcg gcaacgtcga
     3061 agagctggtt agaaccgtta tgtaattggc cagtgtcatt ccggtcgttt caaaaggcag
     3121 cttatcttcc gtgcttttga cgctggcgta gagcagcatg atgagcggca caagaaccag
     3181 ataagagaca acgatgatgg tgaaacccaa ggtaacggct cgaacggaaa atttccgtgt
     3241 ccacgacaac gcctgttttc ggacgccgct atcaagagtg ggttgcgcta cgctcatcga
     3301 tgaattccct tatcggacca tgacgcaaag tcactttgac accaagaacg aacgcacgaa
     3361 gcacctgaac gggtttcatc gcgcccagag actccggctc cgcgatctcg atcgcctctc
     3421 ttataggccc cgtgcttcgc tgcacgcgcg ttgccatcga atcgttagcg cagcgctcca
     3481 cgcagctttc ccatctcgcg ctgaatttcg agaatcgatg tgaaggtctt atcggtttcc
     3541 aagataactg gcggcttctc gttcctaatt ctttcggcaa cgttcccgat tgcgttcttg
     3601 cccacttcca tggcttggcc ccgggtggcg tatcttatct tctgcccttc aggggttccc
     3661 atccagccgg caaaaaacct acccgcatta ggacgcggtg cggttttgag aactccctcc
     3721 gcagacgtca gaaccggcaa tggcgaaacc ggaacccaat ccaccgccgc tccctgtttt
     3781 ttcaaacctt caatcgtaaa tgcgtacgtg cccacggcaa ttgaaacctg cccgccagct
     3841 aacgcattgg caacggtggt gccgccttga acaatcagtg gttgctgctg tgaaactatt
     3901 ttcttggtga gctcgatcgc cttgtccttg ccccattcgg tgccgagcat ggcgaacgga
     3961 accagacgtg cctcgatgat gattttcctg cccttccatt tcggatccgc gagatcctcc
     4021 cagcgcttgg gcgcttcctg ggcactcacg gtcttggtgt tataggcaat gggcaaggtg
     4081 agattatagc cgccgaccaa ccgctggtcg gcatagacag catcgaggcc aaaaattttt
     4141 tcccatcccg aatagggcgc caacatatcg cgatcaatca gagggcgcag cgcccgtagc
     4201 gaccccgatt gtatgacatc gacggcggcc ggcttgccgg cttggctttc agcgattatt
     4261 cgctggacga gcggctccgg gatgctttcg aaggattcga tcttgatccc gggatactgt
     4321 ttatccaacg cctcctgcat gcgttgataa acgatggcat cggttggccc ccagatgact
     4381 actcgccctt ccttcctggc ggcgtggtaa agttccctga tgccgtcgct gtcacccttg
     4441 cctttttctt gggcgcagcc catgtcgcta aagaaaacgc tcatcaaaca gatcataact
     4501 gctgcgacga ttgctctaga tttcataggc caatacctcc cgtctttgcg cctaaaattc
     4561 catcctcaac gttcaatggc tagtgaatcg gatggcgctg caaaaaattt tttaacgcaa
     4621 tcagcgcgcc gccaccgtcc caaaatgtca tatgctacgt tcggaacaag cgccagctcg
     4681 ctgccgggaa caagctctgc aagtcgccgt gcggttgcca ccggagtgct gccgccgcga
     4741 ttagcgtcgt cctccgcgcc ctcgctctct ccagaatcct cgtaaaaaat ctgcacatcg
     4801 tttaaattgg ccgtcggcat tttt
//
LOCUS       DASUTG010000497         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_372812, whole genome shotgun sequence.
ACCESSION   DASUTG010000497 DASUTG010000000
VERSION     DASUTG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372812"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VF089_11330"
     CDS             <1..505
                     /locus_tag="VF089_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6174597.1"
                     /translation="GVFGFLINLPVVSYYEIGTALTANHGHTAMMGVYGMLAVGLLLF
                     CLRYLTRPDRWSDRAAKISFWSLNIGLAWMAFANLFPLGIVQLYYAVDIGYWYARSLA
                     FLNLRWVNVLEWARLPGDALFIIGGALPLLWLCWQALRYPNPRRIEAEAELPKTLYVY
                     DSEPGKS"
     gene            complement(688..1155)
                     /locus_tag="VF089_11335"
     CDS             complement(688..1155)
                     /locus_tag="VF089_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX6174598.1"
                     /translation="MFVWNLLLALGWAAISGDFTLVNVSIGFVVGYGILYLAQRITGR
                     SRYSTKVAELIGFIIYYVWLFLLANLRIAYDIITPKFQIRPGVIAIPLEAKTDLEIVM
                     LANLLTLTPGTISLDVSTDRRFLYIHALYIDDVEKVRRDIKQGLERRLLQVMR"
     gene            complement(1252..1668)
                     /gene="mnhG"
                     /locus_tag="VF089_11340"
     CDS             complement(1252..1668)
                     /gene="mnhG"
                     /locus_tag="VF089_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019569744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX6174599.1"
                     /translation="MKETITSLLMLSGAIFAFLSALGILRFPDLFSRMQAATKGSAFG
                     VTCLLLAVAVHFAELGIATRAIVTIFFVLLTAPVAAHMIGRAAYFVGVPLWQGTITDE
                     LKGHYDMRTHALSSSDRPPSANAAVDQQESAPKQTD"
     gene            complement(1668..1946)
                     /locus_tag="VF089_11345"
     CDS             complement(1668..1946)
                     /locus_tag="VF089_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6174600.1"
                     /translation="MIVSPFESWAGSIALVLLSSALCLGFYRLARGPSLPDRVVALDL
                     IGTISVGFIGVYSIMTKQPVFLDVAIVLALIAFLGTVAFARYLEKGVS"
BASE COUNT          554 a          631 c          637 g          484 t
ORIGIN      
        1 cggagtgttt ggattcctaa tcaatcttcc ggtcgtaagc tattacgaaa tcggcaccgc
       61 gcttacagcc aaccacggcc acaccgccat gatgggagtt tacggcatgc ttgcggtcgg
      121 actgttgctc ttctgcttgc gttacttgac ccgcccggac agatggagcg atcgcgcggc
      181 taagatatct ttctggtctc tgaatatcgg cttggcatgg atggccttcg ccaatctctt
      241 cccgctcggc attgtccagc tctattatgc cgtggatata ggctactggt acgcgcgaag
      301 tctcgctttt ctcaatctgc gctgggttaa tgttctcgaa tgggcgcgcc tgccgggcga
      361 cgctcttttc ataatcggcg gagctttacc gctgctctgg ctctgctggc aggcgttgcg
      421 ctatcccaac ccgcgccgca tcgaagccga agccgagctg ccaaagacgc tttatgttta
      481 tgattcggaa cccgggaaga gctgatggat taaaggatgg ctcggcaatc tgcaggacaa
      541 tcctgtcaac ggcgacgttt ggcgctgcag ccgtaaaaga ctctggtgcg taacgggctc
      601 tgtgggtgtg ctgccagcgg aaatgcacaa ggtgtgcatc gctgtctggt ggagcatccc
      661 ttcggttggt gctgcgttct ggtattgcta tcgcatgact tggagcaggc gccgttccaa
      721 tccttgttta atgtcgcggc gaaccttctc gacgtcgtca atgtagagag catgaatata
      781 aagaaaccgc cggtcggttg agacatcgag gctgatagtt cccggtgtaa gggttaacaa
      841 attagccagc atgacgatct ccaggtcggt ctttgcctcc aagggaatgg caattacgcc
      901 aggcctgatc tggaacttcg gagtgatgat atcgtaagcg atccgtaaat tggctaagag
      961 aaacagccac acgtaataaa taatgaagcc gatcagctca gcgactttgg tactgtatct
     1021 ggaacgaccg gtaatgcgtt gcgccaggta aagtatgccg tagccaacga cgaacccgat
     1081 cgaaacattc accagggtaa aatcgccgct aatggccgcc caccccagtg ccagcaagag
     1141 attccagacg aacatggtga gttgtccttc atatcagtgg tcatgtattc acaagcatac
     1201 ggcatcagct gattatgttg taatcacgta cgcatgtttc aaacgtttat cttaatcggt
     1261 ctgttttggc gcggattcct gctggtcaac ggccgcgttg gcgctaggcg gccgatcact
     1321 actgcttaaa gcatgtgtgc gcatgtcata gtggcctttc agctcgtcgg tgatcgtgcc
     1381 ctgccaaagc ggaacgccga caaaataggc cgcgcggccg atcatatgcg ccgccaccgg
     1441 cgcggtgagg aggacaaaaa agatcgtcac gattgcgcgc gtggcgatgc ccagttcagc
     1501 gaagtgcacc gcaacggcca gcaggaggca agtcacgccg aacgccgaac ccttagtggc
     1561 cgcttgcatg cgcgagaaca aatccggaaa gcgcagaata cccagcgcgg agagaaaggc
     1621 aaaaatggcg ccgctcagca tcaaaagaga agtgatggtt tctttcatca cgagactcct
     1681 ttctccaagt agcgggcaaa ggccacagtg ccaagaaaag cgatcaacgc caggacaatg
     1741 gcgacatcca gaaacaccgg ttgcttagtc atgatgctgt acaccccgat aaagccgacc
     1801 gagatcgtcc cgatcaggtc gagcgcgacc acccggtcgg gaaggctcgg accccgcgcc
     1861 agccgataga atccgagaca aagcgcgctg ctcaataaca caagcgcaat gctgccggcc
     1921 caagactcga acggagaaac aatcatcgca agaacctcca gtacggccga tccagccctt
     1981 gtaaaagcgg cggcgagatt gggcgatgcc gccggcaaaa taacgcatga gtatacgggt
     2041 tccccgatcg ccggacgtcg ttaggctcaa cgtgcctggc ctttccgaat gaacacagcc
     2101 gggccggcga aattacgata cagacgaaca tacttatatt cttttgccaa gcacggcttg
     2161 aatgtactgg cttgggtcca tcaattgctc ggcggcccgt agcgacagga ccagcatggc
     2221 ttccgctccc aatccaaaaa gaacagtcat cagcgccagc aaaactaccg gagccagcag
     2281 caggtagtaa gtcgtcggcg atctga
//
LOCUS       DASUTG010000498         9335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2152704, whole genome shotgun sequence.
ACCESSION   DASUTG010000498 DASUTG010000000
VERSION     DASUTG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152704"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VF089_11350"
     CDS             complement(<1..1949)
                     /locus_tag="VF089_11350"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6174601.1"
                     /translation="MQLDKAYSHKLVEAKWYQHWIESGAFKPSKTDGPYFSMVIPPPN
                     VTGSLHMGHALNNTLQDILCRYKRMDGFRVLWIPGTDHAGIATQNVVERRLAVEGLTR
                     NQLGREKFIERVWSWKQEAGDAILHQLRALGVSCDWDHERFTMDEGLSRAVREAFVRL
                     WDDKLLYRAQRLINWCPRCKTALSDIEVVHQELDGNLWHIGYPLAEDSSRELVVATTR
                     PETMLGDTAVAVHPDDERYQNLTGKSIRLPLSGRIVPLIADPFVDREFGTGALKITPG
                     HDFNDFEIGERFALERISIFDAEGRIDAEAFLQRGETGEWIERYRGKDRLAARRLVVA
                     DLKEKGFLHKIEPHKLSVGHCYRCQTVVEPYLTPQWFVNVTPLAGPAIQAVRHGNIRI
                     IPEGWSNSYFAWMENIKDWCVSRQIWWGHQIPAWYCRSCDSKNIVEGQGGEYGLTKEA
                     QPIVSRAAPQTCPRCGGRDLVQDPDVLDTWFSSGLWPFSTLGWPESTDDLKSFYPTST
                     LVTAFDILFFWVARMVMMGLKFMGDVPFHDVYIHALVRDEQGQKMSKSKGNVIDPLDI
                     MAEYGTDAFRFTLAAMAAMGRDIKLAEERISGYQNFVNKLWNASRFVLMNSAQSPAFK
                     QADEPRALDSAELGFAERWIRSRLAS"
     gene            complement(1954..2472)
                     /locus_tag="VF089_11355"
     CDS             complement(1954..2472)
                     /locus_tag="VF089_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174602.1"
                     /translation="MSKILVVEPYKILQRAIALSFFPEHQVEVTEEIPDMSAIEQRSY
                     DVAIIDAGALREKITAGDLNRRLQGWQIPMIWLEDSTSPVAPVRDNLMVLRKPIARND
                     LLAALSCCQAKVSTIALTDVGEKLRNKTVLVASELKPDPEHTAQIIELVDVVEEKTTD
                     PKRDEPLERKKQ"
     gene            2559..2645
                     /locus_tag="VF089_11360"
     tRNA            2559..2645
                     /locus_tag="VF089_11360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2593..2595,aa:Leu,seq:cag)
     gene            2858..3187
                     /locus_tag="VF089_11365"
     CDS             2858..3187
                     /locus_tag="VF089_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6174603.1"
                     /translation="MISLVIFLMAVGLIACSKDSDQAAEAGKDPDWERGRAVYIANCV
                     ACHNSDPSKDGPIGPALKGSDRELLEARVLTSKYPPNYKPKRPTQVMPQFPFLKSEIP
                     YLAAYLR"
     gene            complement(3197..3892)
                     /locus_tag="VF089_11370"
     CDS             complement(3197..3892)
                     /locus_tag="VF089_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX6174604.1"
                     /translation="MEAAHFFFRWFHFLAGITWIGILYYFNFVQTPFFAETEASVRTG
                     AIQKLVPRALWWFRWGAMITFLAGLLMYLMRLGEMGAGLFFTSSYGVTITLGGLMGTI
                     MFLNVWLIIWPNQQIVIASTNQVASGGQALPAAAGAARRGALASRTNVVFSIPMLFFM
                     GAASHYPSLVGPSAAFGLFWILSLIIIAAVEINPLVGTQGPTKKFLDSVSGALWAGFI
                     LTAIFYVLLLALM"
     gene            4170..5213
                     /locus_tag="VF089_11375"
     CDS             4170..5213
                     /locus_tag="VF089_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6174605.1"
                     /translation="MIHETKSKTLKPSADTFTSLGLRIPSLQGSSDPFRYISEELQLV
                     EETLAHAIRSREQSLTDIAAHIIIGGGKRVRPAVTILAFLAFGGKKASDIVDIAAAME
                     LIHTATLLHDDIIDGAETRRGKDSAFKKFGLKSTLVAGDFLFIKAFEFAGKFDERVVQ
                     WTADACSLLTEGEMLQGHFNRNRDVTLDDYIEIVKRKTASLFHTGGKVGAYLAGADSK
                     QIEATERYGLNMGIAFQVVDDILDIVGHVELLGKPTGMDLRDGNPSLPIILALQARES
                     AVCEAFEAEQPSEQQIAAALQALRTGSAIEAAKTISKRYAEDALKSIKKFPPSLYRNG
                     LKTLVQLIIDRDF"
     gene            5265..5987
                     /gene="ubiE"
                     /locus_tag="VF089_11380"
     CDS             5265..5987
                     /gene="ubiE"
                     /locus_tag="VF089_11380"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX6174606.1"
                     /translation="MAFIENPGNENVRRMFDRIAGRYDLLNGIISFRLDTWWRKKAVS
                     AVVKTGHEKILDLGAGTGDLAFGASRSLRAQGRVVGLDFSFNMLQLARVKQQSRKPNK
                     TDFLQANALMAPFRDDVFDGVMTGFVLRNVPDLRVFFVEAFRVLKPGGRMASLDMFPP
                     SKGLFARLYAFYFYRVMPLFGGFLAKDRQAYQYLSDSVRRFVPPEKVSELLQQCGFEQ
                     VTLQRFLFGAVCLHIANKPHQK"
     gene            6121..6819
                     /locus_tag="VF089_11385"
     CDS             6121..6819
                     /locus_tag="VF089_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174607.1"
                     /translation="MIAALGLWGKTNLYLQTILESFSWIVALEALAHLFFVGFLACYI
                     ASLWGERQAFRAKTFAACILSGLAGLFLSAQSFYEAPIGSVETLVYPLSFFMSALLLG
                     SVTIGMLIGHWYLIDTGQTLDPFIRIYKFFVISLALQSGFSLVSFLLLHFFGTDQSAV
                     ELEKLWTYHSTLLFTRTLIGYLGPLVLSWMIWRTLLIPHTMAATGLFYIALLGVFVGE
                     ILSRQILALTSLPL"
     gene            complement(6799..7107)
                     /locus_tag="VF089_11390"
     CDS             complement(6799..7107)
                     /locus_tag="VF089_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174608.1"
                     /translation="MTDEEEATFPNTDDLFHHGVITRLFPSNNTGIVRIESGREIFFS
                     YDFVILTGEIKSPFHLKVGQPVGYDLGWTPSGLRVTKIKTYLPKRVVQEPDTSKRQGS
                     "
     gene            complement(7104..7532)
                     /locus_tag="VF089_11395"
     CDS             complement(7104..7532)
                     /locus_tag="VF089_11395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX6174609.1"
                     /translation="MDKAIGWARKYSLFPYPFATACCAMEYMSLSMSPYDIDRFGALL
                     PRFSPRQADLLMVIGTVNHKLSPVLKRVYEQMSEPKWVMAFGACAASGGFYDNYATVQ
                     GIDRIIPVDVYVPGCPPRPEAVLDGLMELQKRIANAKQPV"
     gene            complement(7758..7958)
                     /locus_tag="VF089_11400"
     CDS             complement(7758..7958)
                     /locus_tag="VF089_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033015550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX6174610.1"
                     /translation="MATGKVKWFNNSKGYGFIEKEGGGDVFVHYTAIQGDGFKTLNEG
                     QLVEFELAQGDKGPQAINVTKV"
     gene            8699..>9335
                     /locus_tag="VF089_11405"
     CDS             8699..>9335
                     /locus_tag="VF089_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6174611.1"
                     /translation="MAIYTPLAKKDAVKIVDEFALGELLSVTGIKDGSVNTHYLLDTK
                     RGRFFAKIDEIKGELEVKQELDLLFYLKKQGFPCLQPIKSKTGRHYVEINSKILTISR
                     YLEGAELGVEALNATHLTALGNALANLHLIGRGYKKGIDNRFGFNRIVMIYREARRQL
                     PPHLKHIVRVLDDEFSYLENYLDNNLPKGIIHGDLFPDNVKFKGTRLVGVLD"
BASE COUNT         2279 a         2416 c         2296 g         2344 t
ORIGIN      
        1 gaagccaaac gcgaacgaat ccatcgttcg gcaaagccaa gctccgcgct gtcgagagcg
       61 cgcggttcat ccgcctgttt gaaggcaggg ctttgcgccg aattcatcaa aacgaatcgg
      121 gatgcgttcc acaatttgtt gacgaaattc tggtaccccg agattcgttc ttcggccaat
      181 ttgatgtctc gccccattgc cgccatggca gccaaagtga agcgaaacgc atccgtgcca
      241 tattcggcca tgatatccaa aggatcgatg acgttgcctt tggacttcga cattttctga
      301 ccctgctcat cacgcaccag ggcgtgtatg taaacgtcat gaaacggtac atcgcccata
      361 aacttaaggc ccatcatgac catgcgcgca acccagaaaa atagaatatc gaaggcggtg
      421 accaaagtag aggttggata gaagcttttc agatcatccg tggattccgg ccatcccagg
      481 gtcgaaaacg gccacaagcc cgaagagaac caggtatcga gaacgtctgg gtcctgaact
      541 agatcacgac caccgcagcg cgggcaggtc tgtggtgccg cgcgggagac gatgggttga
      601 gcttcttttg tcagcccgta ctcgccgccc tgaccctcga caatattttt actatcacac
      661 gaacggcaat accacgcggg aatctgatgt ccccaccaga tttgtcgcga gacacaccaa
      721 tctttgatgt tttccatcca ggcaaagtaa gaattgctcc atccttcagg aataatgcgg
      781 atattgccat ggcgcactgc ttggatcgcc gggcctgcta aaggtgttac attcacgaac
      841 cactgagggg tgagataggg ctcgacgacg gtttggcagc gatagcagtg gccgacggag
      901 agtttgtgag gttcgatttt atggagaaat cccttctcct ttagatctgc aactaccagc
      961 cggcgcgctg ctaaacgatc cttaccacga taacgctcga tccattcgcc ggtttcccca
     1021 cgttggagaa aggcctcggc atcgatgcgg ccttcggcat cgaagatact gattctttcg
     1081 agagcgaatc gctcgccgat ttcaaaatca ttaaaatcat ggcccggtgt gatcttcaaa
     1141 gctcccgttc cgaattcacg atcgacaaat ggatcggcga ttaacgggac gatgcgtccg
     1201 ctcagcggca gcctgatact tttaccggtc agattttgat agcgctcatc gtcgggatga
     1261 accgccactg cggtgtctcc caacatggtt tccggccggg tcgttgctac cacgagctcc
     1321 ctagaggagt cctccgccaa cgggtatccg atatgccaaa gattaccatc cagttcctga
     1381 tggacaacct caatgtctga gagcgcggtt ttacatcgag gacaccagtt gatcagtctt
     1441 tgcgcgcggt agagtagctt atcatcccac agccggacga aagcttcacg tacagcccgc
     1501 gagagacctt catccatagt gaatcgttca tgatcccagt cgcaggaaac accgagggct
     1561 ctgagctggt gcaaaatggc gtcgccggct tcctgcttcc aagaccaaac tctctcgatg
     1621 aatttttcgc ggcccagctg gttacgagtc aagccctcaa ccgcgaggcg tcgctcgacg
     1681 acattctgtg tcgcaatgcc ggcatggtcg gtgcccggaa tccagaggac gcgaaacccg
     1741 tccatccgtt tgtatctaca cagtatgtct tgcagtgtgt tgttaagggc atgccccata
     1801 tggagcgacc cggtcacgtt ggggggcggg atcaccattg aaaaataagg cccgtccgtt
     1861 tttgacggtt taaacgctcc cgattcgatc caatgttggt accacttcgc ctcgactaat
     1921 ttatgcgaat aagctttgtc cagttgcata gacctattgt ttctttctct ctagcggctc
     1981 gtctcgctta gggtcggttg ttttttcctc gacgacgtct acgagttcga tgatttgcgc
     2041 tgtgtgctca ggatcgggtt tgagttcact ggcaaccaaa actgtcttat ttcggagctt
     2101 ttcaccgacg tcggtcagcg caatggtcga tactttcgcc tggcagcaag acagcgcagc
     2161 gaggagatca tttctcgcaa tcggcttgcg caaaaccatc aggttgtctc gtacgggggc
     2221 gaccggtgac gtactatctt caagccaaat cataggtatt tgccatccct gcagacggcg
     2281 gttcagatcg cctgccgtaa tcttttcgcg cagcgctccc gcatcaatga tggcaacatc
     2341 ataagagcgc tgctcaatcg cgctcatatc tggaatctcc tcagttacct caacttgatg
     2401 ctcgggaaaa aaactcaggg caattgctct ttgcagaatt ttgtagggct caacaactag
     2461 aatcttactc attcgctttc ccggtaagtg gaatcacatc gttgacaagc cattggcccc
     2521 aagctaataa atgaagagcg tgccgcgttc taagcttcgc gaaagtggcg gaactggtag
     2581 acgcgcagga ttcaggatcc tgtgaggtaa cactcgtagg ggttcgactc ccctctttcg
     2641 caccaacaat cattaacata cacgccagga tttctctaat atcactaaat catcttttcc
     2701 gaacaaggta tgctgttaac ccggtgcctc attctcgcca atcaatgaga tagtcgattg
     2761 gttagaaaag gtgtcacctt acgatgccgt cgggtgcgac tgagcggccg agttagcaag
     2821 caggtgcaaa tggacaaaac ctcagatggg aaatgggttg ataagtttgg ttatcttctt
     2881 gatggcggtg gggctgattg cctgttcaaa agattcggat caagcggcgg aggcgggcaa
     2941 agatcccgac tgggagagag gaagagctgt ttacatcgcc aattgcgtcg catgtcacaa
     3001 cagcgatcca tcgaaagatg gtccgatcgg gccagccctg aaaggatccg atcgggagtt
     3061 gctggaagcc cgggtgctaa ccagtaagta cccgccgaac tataaaccga aacgcccgac
     3121 tcaggtaatg ccgcagtttc cgtttttgaa gtcagaaatt ccctatcttg cagcctacct
     3181 ccggtgacgc ttgcccttac atcagggcaa gcagtaagac gtaaaatatc gcggtaagaa
     3241 taaagccagc ccacagcgcg ccggaaacag aatccaaaaa tttcttggtt ggtccctgcg
     3301 tcccgaccag cgggttaatc tccaccgcgg cgataatgat cagcgacaaa atccagaaga
     3361 ggccgaatgc ggccgatggt ccgaccaatg atgggtaatg actcgcagca cccataaaga
     3421 acagcatcgg aatagaaaaa acgacgttgg ttctcgacgc cagagcgccg cgtcgtgccg
     3481 cccccgcagc tgcaggcaat gcttgccccc cgcttgccac ttgattcgtc gatgcaatta
     3541 caatctgctg attgggccaa atgatcaacc agacattaag aaacatgata gtccccatta
     3601 ggccgcctaa agtgatggtg actccgtagg acgaggtgaa gaatagtccg gcacccatct
     3661 cgccaagacg catcaggtac atcagcaaac cggctagaaa cgtgatcatc gccccccagc
     3721 gaaaccacca tagggcgcga ggaacaagct tctgaatggc gcctgtacga acgcttgctt
     3781 cagtttctgc aaaaaacggt gtctggacga aattaaagta atacaagata ccgatccagg
     3841 tgatcccagc caagaaatga aaccatcgaa agaaaaaatg cgctgcttcc atagagctcc
     3901 cctcctccta accgttaact ggatttacca gaagtaattg ctttttatat attagggcgt
     3961 cggcgaattt gtcaaggcaa acgtcgacct gtcataactc gacgagttga tcaacgcttt
     4021 agaactccgg gcgcatagcg gacaacgcat cgatttctgg agtaaacctg atgatctgca
     4081 ccgatcaaga tgagtcgaca tctgcttgac ctgcttaccg atctatgaca tttttctgct
     4141 aactctctcg cctccgcaac ggagttcttt tgatccacga gacaaaaagc aagacgttaa
     4201 aaccttccgc ggataccttt acgagtttag gcctcagaat tccttctcta cagggcagca
     4261 gcgacccgtt tcgctatatc agcgaagaac ttcaactcgt cgaagagact ctggcgcacg
     4321 cgattcgttc ccgagagcaa tcacttactg atattgcagc tcacattatt attggcggtg
     4381 gcaagcgcgt gcgtcccgcg gtaacgattc tggctttttt ggcgttcggg ggtaaaaaag
     4441 ccagcgatat cgtcgatatt gctgccgcta tggaacttat tcacacggca accctgcttc
     4501 acgatgatat cattgatggt gctgagaccc ggcgcggcaa agactccgct tttaaaaaat
     4561 tcggtcttaa atctacgcta gtcgcaggtg attttctttt tatcaaagcc ttcgagtttg
     4621 ccgggaaatt tgatgagagg gttgtgcaat ggacagcgga tgcctgctca ctgctcaccg
     4681 aaggagagat gctgcagggc catttcaatc gcaaccgaga cgttaccctt gatgactata
     4741 tagaaatcgt caaaagaaaa accgcatcgc tctttcatac cggaggcaaa gtcggcgctt
     4801 atctcgccgg cgcagactca aagcaaattg aggcgactga gcgctatggc ctcaatatgg
     4861 ggatcgcctt tcaggtcgtc gacgatattc ttgacatcgt tggacacgtc gagctgttag
     4921 gaaaacctac cgggatggat ttgcgcgatg gcaatccttc cttgccgatc attctcgcgt
     4981 tgcaagccag ggaaagcgcg gtttgtgaag cgttcgaagc ggaacagccg agcgaacagc
     5041 aaattgccgc tgccctccag gcgctgagaa ccggaagcgc tatagaagca gccaaaacca
     5101 tttctaagcg ctatgctgaa gatgccttga agtccatcaa gaagttccct ccttctcttt
     5161 atcgcaatgg attgaaaacc ctcgtccaac tcatcatcga ccgagatttt tgaccgacgc
     5221 ggccgcccta acgttcggga ttccataccg ctgagcatcc atagatggca ttcatcgaaa
     5281 acccgggcaa tgagaacgta cggcgcatgt tcgatcgaat tgcaggtcgc tatgatctac
     5341 tcaatggaat catcagtttc cgactggata cctggtggag aaagaaggcc gtttccgcag
     5401 ttgtcaaaac cggccatgag aaaatcctcg atctcggcgc tggaactggg gatctggcgt
     5461 ttggcgcgag cagatcgctc cgtgcgcaag gcagagtcgt tgggctcgat ttttccttta
     5521 acatgctaca gctggcgcgc gtcaagcagc aaagccgaaa gcccaacaaa acagattttc
     5581 ttcaggcgaa cgcactcatg gcgccgttta gagacgatgt attcgatggg gttatgactg
     5641 gattcgtatt gcgtaatgtt cctgatttgc gggtattctt tgttgaggct ttcagagtcc
     5701 taaaaccggg cgggcggatg gcttctttgg atatgtttcc gccttcgaag ggcctgttcg
     5761 cgaggctata tgccttctat ttttatcgcg tcatgccatt attcggcggt tttctcgcca
     5821 aggaccgcca agcatatcag tatctgtcgg actccgtgcg ccggtttgtg cctccggaaa
     5881 aagtatccga actacttcaa caatgcggct tcgagcaggt cacgctgcaa cggtttctat
     5941 tcggcgccgt gtgccttcat attgcgaata aaccgcatca aaaataagat gcgaggtttc
     6001 tccagcagct ttctactttt tttctatcaa attgctctcg gcggcctctt cggattggca
     6061 tttaccccat tccacgaact cgatcgagcc ttttacaaat caaccggcgg cgtgctgttt
     6121 gtgattgcgg ccctgggcct ctgggggaaa actaaccttt atttgcaaac catattggag
     6181 agctttagct ggatcgtggc tttggaagca cttgcccacc tcttcttcgt cggcttcttg
     6241 gcctgctata tcgcctcgct ctggggtgag agacaagcct ttagagctaa gacgtttgca
     6301 gcttgtattc tttcaggtct cgctggcctt ttcttgagtg ctcagagctt ttatgaggcg
     6361 ccaattggct cggtcgaaac cttggtctac ccgctgtcgt tctttatgtc agcgctctta
     6421 cttggcagcg tgacgatcgg aatgctcatc gggcactggt acttaatcga taccggtcag
     6481 acacttgacc catttatccg tatctataag ttttttgtca tctctcttgc gctccagagt
     6541 gggttctcat tagtgtcttt cttgttgctg cacttttttg gcaccgatca aagtgctgtg
     6601 gaacttgaga aactctggac gtatcactca accttgttgt tcactcgcac attaatcggg
     6661 tacctggggc cattggttct ctcctggatg atttggcgaa ccttgctgat tccgcacacg
     6721 atggcggcaa cagggttgtt ctatatcgca ctgctcggtg tatttgtggg agagatttta
     6781 agtaggcaga ttttagcgct aacttccctg cctctttgaa gtgtccggct cttgaacgac
     6841 tcttttcggc agataagtct ttattttggt gactcgaagc ccgctaggag tccaccctaa
     6901 gtcgtaaccc accggctgtc cgacctttag atggaatggc gatttgattt cgccggttag
     6961 aattacgaaa tcgtacgaaa agaagatttc tcggccgctc tcgattcgca cgataccggt
     7021 attattcgac gggaacagtc tagtgatcac gccgtggtgg aacaagtcat ccgtgttcgg
     7081 gaaagtggcc tcttcctcat ctgtcatact ggctgctttg catttgcaat ccgcttctgc
     7141 aactccatta ggccatcaag aactgcttca ggccgcggcg gacagccggg gacataaacg
     7201 tccaccggaa tgatacgatc aattccttgg acagtggcgt aattgtcata gaaacctccg
     7261 ctggccgcgc atgcgccaaa agccataacc cacttgggtt cagacatttg ctcatagaca
     7321 cgctttaaaa ccggcgatag cttgtgattg acggtgccga tcaccatcaa cagatctgcc
     7381 tgacgcgggc taaagcgtgg caaaagagcg ccaaaacgat cgatatcgta gggggacatc
     7441 gataaagaca tgtactccat cgcacagcat gctgtagcaa acggatatgg gaataaggaa
     7501 tatttccgcg cccacccaat cgccttatcc aaacgggtta ggactactgt ctcatttagg
     7561 agccggtcct gcgcgaactt ggattcatct accgtattca ccctatccac ttgcatgaac
     7621 tagtatccct ttcttcaatt cccttctcac cgaaagcgat tgagttcaac tatcagtcag
     7681 tactattcaa tggctacaag catggccgac aatcgccgat tagcgattat cttgttcatc
     7741 ctgggagttg ttgtcggcta caccttagtg acgttgatag cttgcgggcc tttatctccc
     7801 tgggccaatt cgaattcaac taactggcct tcgttaagag tcttgaatcc atcgccttga
     7861 atggccgtgt agtgaacaaa cacatcaccg cctccttcct tttcaataaa accatatccc
     7921 ttggaattat tgaaccactt taccttcccg gttgccattc ccttagaacc tcctggtcca
     7981 cgttgccggc tacagcatgt ggaatccaac tcatacggta tttaacgatc cgttggcgtg
     8041 tcccaatact gacccgccag cacattagaa aaacatatgt agaattgtca agagaaaagt
     8101 ttctccacat ggttgtctaa caacggcttt cttaacactt gactaagaac ccggtaaatg
     8161 acagctgatc cgccgcatca accaagtccc acaattggat caagttgcag tgtggacatc
     8221 caagctaaag tagtccttag gaatcgctcc tggatctggt gttgtcgatt ttctcctccg
     8281 cggaactttg gtcctgagag caaggggaca gctccttccg tgaccgctat tgaacgacat
     8341 agttggtaat tgccgtaaca tcccgaacac ctatgtgcca caatcccccg aacagtttgc
     8401 cgcaggagag cccatggttc gctgccacac aaggaccgtc gccgccaact ggagatgctg
     8461 gtttccctcc gaagattcat tgattcatct agacgcacag tagacaagga tcaatatgac
     8521 taccccgttc gggaggaagt gactttgggg ttcctcaggc attaatgttc ggaaggtagt
     8581 gggaactgcc catttagtgg ttgacataat gatcattttt tgattatatt tagcggggtt
     8641 agcggatctg gtgaggaact ttcgttaaaa agctgaaatc ggtcgataac ttaacagaat
     8701 ggctatctat actccactgg cgaaaaaaga cgctgtcaag atcgtcgatg agtttgccct
     8761 tggcgagctt ctcagtgtca ccggaatcaa agacgggtct gtgaatacgc attatcttct
     8821 tgacaccaag cgcggacgat ttttcgcgaa aatagacgaa atcaagggag aactagaagt
     8881 caaacaggag cttgatttgc ttttctactt gaaaaagcag ggctttcctt gtttgcagcc
     8941 gatcaagagc aagaccggcc gccattatgt tgaaattaat agcaagatac tgaccattag
     9001 ccgttacttg gaaggggccg aacttggggt cgaggcgctg aatgcaaccc atttgacggc
     9061 gttgggcaat gctttagcca atctccatct catcggccgg ggctataaaa aagggataga
     9121 taatcgcttt gggtttaacc ggattgttat gatctatcga gaggcgcggc gtcaactccc
     9181 gcctcatcta aagcatattg tccgggttct agacgatgaa ttctcctacc tcgagaatta
     9241 tctagacaac aatttgccca aaggcattat tcatggagat ctctttccgg acaatgttaa
     9301 atttaagggt actcggttgg ttggtgtttt ggatt
//
LOCUS       DASUTG010000499         7294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1113212, whole genome shotgun sequence.
ACCESSION   DASUTG010000499 DASUTG010000000
VERSION     DASUTG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113212"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1106)
                     /locus_tag="VF089_11410"
     CDS             complement(144..1106)
                     /locus_tag="VF089_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174612.1"
                     /translation="MTVFVLAICGDVARAQQTKKVPRIGYLSPLSASSGRAGLQAFRQ
                     GLRESGYVEGKNILIEYRWSEGGKLDRLPELATELVSLGVDVIVTSGTPPVLAAKQAT
                     NTIPIVAANADNLVELGVVASLARPGGNVTGSTRVDADFSAKRLELLKETLPKLSRLA
                     VLSHGALGGDQEELKEIQAAAEPLGVQIHSVTAQEPDQFSGAYAEMIRRRADALVFFT
                     SAFTAFHRSQLVELAIKNRLPTMCGGANWTDVGCFMSYGPNVIELYRRAAVFVDKILK
                     GTKPADIPIEQPTKFEFIVNLRTAKQIGLTIPANVLARADRVIK"
     gene            complement(2023..3477)
                     /locus_tag="VF089_11415"
     CDS             complement(2023..3477)
                     /locus_tag="VF089_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX6174613.1"
                     /translation="MRLRYRRALEFVALGLMMFLAPGVDWGRSGYAAGPADPANVATS
                     LADQEAVSITVYNSNIGLVKDARRLSLPSGITQLNFGEVAAKILPHTVHIKSLSDPQR
                     LQILEQNYEYDLLTPQKLLEKFVGKEITILKDGVEVPITILSMNQGMVYKLGGRILTG
                     QPHNLIFPNIPNNLISHPTLVWLLDNDSTAPHRIEATYLTQGLGWRADYVAVLDSKDK
                     LLDLSGWVTLDNQSGATYQNARLKLVAGDVNRVIERRGAADAVRELSELSAKPATAPF
                     TEQSFFEYHLYSLQRATTIKENQTKQVSLLSADRVPITKQYIYRGSQQYFRSRYNAPL
                     TNQKVGVFVEIANKKEQNLGMPLPKGVVRVYKADNDGSLQFIGEDRIDHTPKDETIKI
                     KMGDAFDVVGQRKQTDWRKLADNLYEAGFEISVRNHKEDAVTVSVIEPMLADWEILSS
                     SHAHKKTDAHTAQFDLPVAKNGETKLQYRVRYKF"
     gene            complement(3713..4888)
                     /locus_tag="VF089_11420"
     CDS             complement(3713..4888)
                     /locus_tag="VF089_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174614.1"
                     /translation="MNCVEAQKHFADLLDHRRDERLRKVNDHLTACDRCTEELAALAA
                     CQQLVSGLPPVEPPVGFTTRVMAEIRDTVHRPNTWQRLFLGTQKLPIQAAAVILISVL
                     AVFIYQKESRQPEWTTTVPPASPLQKHDEADKLPAAAGGAPAPELKVKESSEAGAQER
                     RVKRSPQTEQLRSRAKPEEQSNIIGGVQPGVGKVAPPAASVNPAKVAPSEPKREASPA
                     DETGSVRQEQSLRSGEAQAEGAPSSAPLRDSPSSAGPEEKRARSSFDALSSGTAGFSD
                     RKLILRVKQPARDDSSTERLSELERPDAEASPSQIFKDLERGRQRAIQTGQPQTIAAI
                     IDASQYDEFKKELARLGKIESEAPAPANEIDVFSKSSGQLRLTITVLPPNSSLQPTQ"
     gene            complement(4885..5463)
                     /locus_tag="VF089_11425"
     CDS             complement(4885..5463)
                     /locus_tag="VF089_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6174615.1"
                     /translation="MLGEDTGDAECVRRVQRGDIEFFEILVRRHQNTTFNLIYRFLGD
                     YDEASETAQEVFLSAYKSIQQFRGDANFATWLYRIAFNHASTRRKNLSSKRQRQVALE
                     DDAALVDSGANPETCAEQKEIQQYVQQALNSLDGDDAQIILLRDLQDVSYDDIAQTLD
                     VPVGTVKSRLHRARQALRISLAPYFTRDRKVS"
     gene            complement(5478..5714)
                     /locus_tag="VF089_11430"
     CDS             complement(5478..5714)
                     /locus_tag="VF089_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174616.1"
                     /translation="MIRRLALGAYGVKSYSQPVKTVIPAWFYGNLGETGTGPPIKEFG
                     GDDFRVSCDVLITREKLRLIADLGQRELFPNRAV"
     gene            complement(5711..6643)
                     /locus_tag="VF089_11435"
     CDS             complement(5711..6643)
                     /locus_tag="VF089_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174617.1"
                     /translation="MIRIAVPDLVSNSYFPAIAAVELGFFKSEGLDASIDLLFPVPKT
                     FAALRDGQLDFVVGSAHATLLAFPDWNGAKLLAAIAQHTYWFLVIRKDLNPQRGDLSI
                     VKGLRIGAAPGVDLSLQRMLVEAGIDPAKNNVQIMPIPGAAGPNVSFGLSAAKALEDG
                     KLDGFWANGMGCEVALRRGVGTMVIDVRRGDGPPPARHYTFPALVTTEKRIEQEPETA
                     AAAIRAIIKAHKALKQDINLATDVGRKRFPPAEAELIAELIRRDLPYYDPTITEEKIA
                     SMNRFASDIGLLSGPVTYDKVVATQFNHLWKETV"
     gene            complement(6798..7148)
                     /locus_tag="VF089_11440"
     CDS             complement(6798..7148)
                     /locus_tag="VF089_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174618.1"
                     /translation="MNIVRLYTGKDQKSHFEEIELNFGGNQPMLTTESRPATSAVFRS
                     APAGLFLDRHPAPRRQFLVTLSGSWEIEVSNGVKRVFKTGDVMLADDTTGEGHTSRVL
                     GNEPHVFMTVPLAD"
     gene            complement(7180..>7294)
                     /locus_tag="VF089_11445"
     CDS             complement(7180..>7294)
                     /locus_tag="VF089_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6174619.1"
                     /translation="MDGEVILGAAGASGATADEDEACVIAGIQAAGLQFKV"
BASE COUNT         1569 a         1984 c         1950 g         1791 t
ORIGIN      
        1 atattcgatt ttggatgctt cgacgtagct cggtacaggt tttggacttt agattaccgg
       61 aacaagaatt cggaagtcgg agtaaagtct tttcgctcat tcgtttgctc cttgatcgaa
      121 aatccaaaat cgcaaatcca aaatcatttg atcaccctgt ccgcacgcgc caacacgttc
      181 gctggaatgg taaggccgat ctgcttcgcc gttcttaagt tcacaatgaa ctcgaacttt
      241 gtcggttgtt cgattggaat gtcggctggc ttcgttcctt tcaagatttt gtccacaaag
      301 acggcggcac gccggtatag ttctattacg tttggcccgt aggacatgaa gcaaccaacg
      361 tcggtccagt tagctccgcc gcacatcgtc ggcagccggt tcttgatggc aagctcaaca
      421 agctgacttc gatggaatgc ggtaaaggcg ctggtaaaga aaacaagagc atcggcacgt
      481 cgccttatca tctcagcgta ggcacctgaa aactggtcgg gctcttgcgc cgtaactgaa
      541 tgaatctgga cccctaaagg ctcggctgcc gcttggatct ctttcaattc ctcctgatct
      601 ccgccgagag ctccatggga aagaacagct agacgagaaa gcttgggaag ggtctccttg
      661 aggagctcga gccgctttgc actgaaatca gcgtcaactc tcgtcgatcc cgtgacgttc
      721 ccaccggggc gagcgagact cgcaacaaca cccaactcca caagattatc tgcattggcc
      781 gcgacaatgg gaatcgtgtt ggtcgcttgc ttggcagcaa gaactggcgg cgtgccggat
      841 gtgacgatga catctacacc caggctaaca agctcggttg caagctccgg aagccggtca
      901 agcttccctc cctcggacca tctgtattcg atgagaatat tctttccttc aacatagccg
      961 gactctcgta ggccctgccg gaaagcttgg agcccggctc tgccgctgga agcggagagc
     1021 ggcgagagat accctatacg agggaccttc tttgtctgct gcgcgcgagc tacatcccca
     1081 cagatcgcca gcacgaatac ggtcaccaaa agccccagcc gttttagatc gtcgctactt
     1141 cgtcggagtt tatcctgatc ggaactgaag ggctcagtac aggtcttcga tctctgattg
     1201 tcggatcgaa accgaaggat tccgatccga aaggttttcc tcatgtgtca gttcgcttaa
     1261 tccaaagtcg gcaatgtaaa tcagaattct tgccctgcct aatccagcgg atcatcagag
     1321 ttagttttcc gctagttccc aagaaaatct catagagctc aaactaaaaa gcccgaccgg
     1381 ttagaaacca atcgggccca attaagaggg aagggtaagg gttgcagcct gaacagcgtc
     1441 agccggaatg ttttggttac tgataatttg cgaccgcgtt aacagagcgc ttcactcaag
     1501 atcgtcagcc gccttataga tcacctccga atcgcttatt ctgctttctg tcaaagcgaa
     1561 ttcatccgca acaccctgat gaaaacattc ctagagcaat tgccaggcca caggcactaa
     1621 acaactcggc atctgaactg atggagaatt tgtttcattc ccagttactc gatcggccgc
     1681 gaagcaaacc actgtatcgt tttgactacc gggcagagca aatttgattc gctttctctg
     1741 gtctgccaag agggcgccac tgaagcatcg ctcagagaac ccccagggtg aggagatgat
     1801 accgatgaaa aatgcggaac gggtcgctta actctaccta acaaaggtta tcaagctgaa
     1861 gagcgtccat cggattgtgt cgctacggcc ttaacgcacc gttggcgaac cactgtcaat
     1921 ggctaatgac aaggatgaaa acggaacttt aaaagcggaa ttcaaactcc agctttcata
     1981 agtcgtccct ttcttacgcg tatgggtcgc tttcagagcc tgctagaatt tatatcgcac
     2041 tcgatactgc agtttagtct caccgttttt cgccacgggc agatcgaact gagccgtgtg
     2101 ggcgtcagtt ttcttgtgag catgagaact gctcagaatc tcccaatccg ccagcatcgg
     2161 ctcgatgacg cttaccgtga cggcgtcttc cttgtgatta cgcacgctga tttcgaaccc
     2221 ggcttcgtaa agattgtcgg cgagcttgcg ccagtcggtc tgcttgcgct ggccgacaac
     2281 gtcaaaggcg tcgcccatct tgatcttgat ggtttcgtct ttgggcgtgt gatcgatgcg
     2341 gtcctcgccg ataaattgca agctaccgtc gttatcggct ttgtaaaccc gcacgacccc
     2401 tttcggtagc ggcatgccca ggttttgttc cttcttattg gcgatttcaa cgaacacccc
     2461 aaccttttga tttgtcagcg gcgcgttata cctgctccgg aagtattgct gtgagccacg
     2521 atagatatac tgtttggtta tgggaacccg atccgctgat agtaggctca cctgttttgt
     2581 ctggttctcc tttatcgtcg tagctctctg caggctgtag aggtgatatt cgaagaacga
     2641 ttgttcagta aaaggagccg tggccggctt tgccgagagt tccgacagct cgcgcacggc
     2701 atctgcggct ccccgtcgtt cgatgacccg attgacgtca ccagccacaa gcttgaggcg
     2761 tgcgttttga taggttgctc cgctttgatt gtccagggtc acccatccgg aaaggtccag
     2821 cagcttatct ttactgtcga gaaccgcgac gtaatcggcg cgccaaccga ggccttgggt
     2881 aagataggtt gcctcgattc ggtgcggcgc ggtgctgtcg ttgtccaata accaaactaa
     2941 agtggggtgg gagatgaggt tgttgggaat gttcggaaag atcagattat gcggctggcc
     3001 agttaaaata cgtccgccca acttataaac catgccctga ttcatgctga gaatggtgat
     3061 cggaacctcg accccgtctt tgaggatcgt gatttctttg ccgacgaatt tctcgagcag
     3121 cttctgcggt gtgaggagat cgtactcgta attctgttcg agaatctgca aacgctgggg
     3181 atcgctaagc gattttatgt gcacggtgtg cggcaagatt ttcgccgcca cttcgccgaa
     3241 gtttagctgg gtgatcccgc tgggcaaact cagccgtcgc gcgtctttga ccaagccgat
     3301 attgctgttg tagacggtaa tcgaaaccgc ttcctgatcg gccaggctag ttgctacgtt
     3361 tgcagggtcg gcgggcccgg ctgcatagcc cgatctgccc cagtccactc ctggcgcaag
     3421 aaacatcatt aaaccgagcg ccacgaattc taatgccctt ctgtagcgta atctcactgt
     3481 tcctcctgag gcggatgaaa cccgccgttg cgatttcttg gattagactt cgcacggagg
     3541 aaaattgttc ccgagcatgc cgctgaaaaa gcctcatatg cttcgttccg cttcggccga
     3601 ctcgcttcaa cgtaccgaag cgtacgcctc agctcatcga acttcgcgcg cgttgcctat
     3661 aagtcttgtt gagcagcctg cataaacttt ggcagcagac tgcgcgagat tgctactgtg
     3721 ttggctgaag ggaagagttc ggcgggagaa cggttatcgt gagcctgagt tggcccgatg
     3781 attttgagaa aacgtcgatc tcgtttgccg gcgcgggcgc ttcgctttcg atcttcccca
     3841 aacgggcgag ctctttcttg aattcatcat actgactggc atcgatgatc gcggctattg
     3901 tttgtggctg acccgtttga attgcgcgtt gtcgcccgcg ctcgaggtct ttaaatattt
     3961 gtgacggtga tgcttccgca tcagggcgct ccaactccga taggcgttct gtgctgctgt
     4021 cgtcgcgcgc cggttgcttc acacgcagaa tcaacttgcg atccgagaac cctgcagtac
     4081 cggaactaag agcatcaaac gatgagcggg cccttttttc ttccggcccg gcagaagaag
     4141 gcgagtcgcg aagcggagcg gatgaagggg ctccttccgc ctgtgcctct cccgatcgca
     4201 atgactgctc ctgtcgaact gaaccggttt catctgccgg cgacgcttcc ctcttcggct
     4261 ctgaaggcgc gactttagcc gggttaacag aggcggcagg cggagcaact ttgccaacgc
     4321 caggctggac accgccgatg atgttgctct gttcttctgg tttggcacgt gatcgaagct
     4381 gttctgtttg aggtgaacgt ttcactcttc gttcctgcgc tcctgcctcg ctggattctt
     4441 tcaccttcaa ttccggtgca ggagctcctc ccgccgccgc cggcaatttg tccgcttcat
     4501 cgtgtttttg aagtgggctc gcaggcggaa cggtcgtagt ccactccggc tggcgagact
     4561 ctttctgata gatgaaaacg gccagaacgc tgatcagaat taccgccgca gcctggatcg
     4621 gaagcttttg cgttcctaag aatagccgct gccatgtgtt gggccgatgg accgtgtcac
     4681 gaatttctgc catgacccgg gttgtaaagc ccaccggcgg ttccaccggc ggcaagcccg
     4741 aaactaattg ctggcaggca gcaagagcgg ccaactcctc ggtgcaccga tcgcatgcgg
     4801 tgagatgatc gtttactttg cgcagccgtt cgtcgcggcg gtgatccaac aaatctgcaa
     4861 agtgtttctg tgcctcaacg caattcatga aactttccga tcgcgggtaa agtatggcgc
     4921 aaggctgatt ctcaacgctt gtcgcgcacg gtgcagccgc gatttcaccg tgcctacggg
     4981 aacatcaagc gtttgggcga tgtcatcata agaaacatct tgtaaatccc gcagcagaat
     5041 tatttgcgcg tcgtcaccgt ccaggctgtt taaagcttgt tggacgtatt gctggatttc
     5101 cttttgctcc gcgcaggtct cgggattagc gccgctgtca accagcgccg catcgtcttc
     5161 cagtgcaacc tgtcgctgtc ttttgctgct caagtttttg cggcgggtgc tggcatggtt
     5221 gaaagcgatg cgatagagcc aggtggcaaa gttggcgtcg ccgcgaaact gctgaatcga
     5281 tttgtaagcc gagaggaaca cttcttgcgc cgtctcgctt gcttcatcat agtcgcccaa
     5341 aaatcgatag atcaggttga aagtcgtatt ctggtgccgc cgcaccaaaa tttcgaagaa
     5401 ttcgatgtcg ccccgttgaa cgcgcctgac gcattccgcg tcacctgtat cctcaccgag
     5461 catggacttg ttttgtatta gacggcccgg ttgggaaaaa gttcccgttg gcctaaatcg
     5521 gcaattagac gtagcttttc gcgcgttatc agaacatcac acgatacccg aaagtcgtca
     5581 cccccgaatt ccttaatcgg gggtccggtt ccggtttcgc ctagattgcc atagaaccat
     5641 gcgggaatga cggttttaac cggctgggaa taactcttta cgccataggc gccaagagct
     5701 aaccgcctga tcaaaccgtt tctttccaca ggtggttgaa ctgagtcgcg accactttgt
     5761 cgtaagtcac cggacctgac agcagtccaa tatcgctagc gaagcgattc atgctggcaa
     5821 ttttttcttc cgtgatggtc ggatcgtagt agggaagatc gcgccggatc aactcggcga
     5881 taagttcggc ttccgcagga ggaaagcgtt ttctgcccac gtcggtagcc aaattgatat
     5941 cttgtttgag cgctttgtgc gccttgatga tcgcgcggat cgccgcggca gccgtttcgg
     6001 gttcctgttc gattcttttt tccgtagtca ccaatgccgg aaacgtatag tggcgcgccg
     6061 gaggagggcc gtcaccgcgt cgcacatcga taaccatcgt tccgacgccg cgccgcaggg
     6121 cgacttcgca tcccatgcca ttggcccaga agccgtcgag cttgccatct tccagagcct
     6181 ttgccgccga caggccgaaa gaaacgttgg gcccggcggc gcccggaatc ggcatgatct
     6241 gcacattgtt ttttgccgga tcgatgcccg cttcaacgag cattcgctgc aagctcaaat
     6301 caaccccggg ggcggcgcca atccgcagtc ctttgacgat gctcaagtca ccgcgctgcg
     6361 gattgagatc cttgcgaatc accaaaaacc agtaggtatg ctgcgcgatt gcggcgagta
     6421 atttggcgcc gttccaatct ggaaacgcca atagcgtggc gtgggccgag ccgacgacga
     6481 aatcgagctg gccgtcgcgc agtgcggcga aagttttcgg caccgggaac aataaatcga
     6541 tgctggcgtc gagtccttca gatttgaaaa agcccagctc gacggcggca atggccggga
     6601 aatacgagtt cgatactaaa tcaggtacgg cgattcggat catgactgac ctctataaaa
     6661 tttttcgttg ggtcgtaatc tgatgttgaa cgttgaacaa ttgaacggcg ctcacacgta
     6721 ggttcaaatc gttccagtcg tttcaatcgt tccaatggtt caaacattca gcttgcgcac
     6781 tgagtgggcg tcgcgcgtca gtcggccagc ggcacggtca taaaaacatg cggctcattg
     6841 cccagcacgc gggacgtgtg accctcgccg gtggtgtcat cggcaagcat gacatcgcct
     6901 gtcttgaaga ctcgcttgac gccgttgctc acttcgattt cccacgagcc ggatagggtc
     6961 accagaaatt gccgccgcgg cgccggatgg cgatcgagaa aaagcccggc cggcgcggag
     7021 cgaaagaccg cgctggttgc cggtcgagat tcggtcgtaa gcatcggttg attgccgccg
     7081 aaattgagct caatttcttc gaaatgcgac ttctgatcct taccggtata cagcctgacg
     7141 atgttcattt gtgctcctat cgattaagtt agctgcgcat taaactttga attgaagccc
     7201 ggctgcttga attccggcaa tgacgcacgc ctcgtcttca tcggcggtcg cgccgctggc
     7261 gcctgcggcg ccaaggatta cttcgccgtc catg
//
LOCUS       DASUTG010000500         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2003253, whole genome shotgun sequence.
ACCESSION   DASUTG010000500 DASUTG010000000
VERSION     DASUTG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003253"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF089_11450"
     CDS             complement(<1..187)
                     /locus_tag="VF089_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6174620.1"
                     /translation="MERIPEPELMDEDEQARAYALADFAEPHNHFVELFRECFPDEAI
                     TGHVLDLGCGPADVTLRF"
     gene            complement(200..1450)
                     /gene="lysA"
                     /locus_tag="VF089_11455"
     CDS             complement(200..1450)
                     /gene="lysA"
                     /locus_tag="VF089_11455"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6174621.1"
                     /translation="MDHFHYRDGRLFAEEVDLATVAAEYGTPCYVYSRATIEHHWRAF
                     DNALASRRHLVCYAVKANSNLAVLNTLTRLGAGFDIVSVGELERVLRAGGDPAKVVFS
                     GVGKRTDEIRRALEVGIRCFNVESEAELHRLNAVAGELGRRAPIALRVNPDVDAGTHP
                     YISTGLKENKFGIDIEAAPAIYTQAARLLHIEVVGVDCHIGSQLTSIAPFVDALDRVL
                     LLLDQLAEQGIAIEHLDLGGGLGIRYRDENPPLPTEHADAVLARLADRPQEILIEPGR
                     AIVGNAGVLLTRVEHLKCTPHRNFAIVDAAMNDLMRPALYGAWQRILPLVPRASGTTR
                     TYDVVGPVCETGDFLGKERELVLEPDDLLAVRSAGAYGFAMSSNYNTRPRAAEVMVDG
                     DRVYLVRQRETLDDLMAGESLLPE"
     gene            complement(1523..1669)
                     /locus_tag="VF089_11460"
     CDS             complement(1523..1669)
                     /locus_tag="VF089_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018867637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX6174622.1"
                     /translation="MKPCWAQYLLWFVIAVLGTGAMLSGCGQKGDLYLPDQPPKKQQH
                     SSQG"
     gene            1863..2063
                     /locus_tag="VF089_11465"
     CDS             1863..2063
                     /locus_tag="VF089_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174623.1"
                     /translation="MAVALLLVALAVNLQTYQRLMSEQEVAELAFQAWGSQHYQVHLQ
                     YPSGQMLLSICAATSGNWTRTF"
     gene            2402..>2743
                     /locus_tag="VF089_11470"
     CDS             2402..>2743
                     /locus_tag="VF089_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6174624.1"
                     /translation="MRLKTNIFLWVSLATVLPLTFLVLGATAYSERIYREKVVGEVNA
                     SLNNVIAEIDRRLRVEREMVLALTEAPAMRRFFPVLERAAVGEVHAEFSRRSDEFNAF
                     LEAFQNIVPSLS"
BASE COUNT          479 a          827 c          910 g          527 t
ORIGIN      
        1 caaagcgcag ggtgacgtct gccggcccgc agcccaaatc caggacgtga cccgtgatcg
       61 cctcatcggg gaagcattcg cggaagagtt cgacaaagtg gttgtgcggc tctgcgaaat
      121 cggccagcgc ataggcgcgg gcctgttcat cctcatccat cagctcaggt tcgggtatgc
      181 gctccatgtg gactccgtac tattcgggta gcagggactc gcccgccatc aggtcatcga
      241 gagtttcgcg ctgccgcacc agatagacgc ggtcgccatc caccatgacc tcggcggcac
      301 gcgggcgggt gttgtagttg gagctcatgg caaagccgta ggcgcccgcc gagcgcaccg
      361 ccagcagatc gtccggctcg aggacaagct cgcgctcctt acccagaaag tcgcccgttt
      421 cgcacaccgg gcccaccaca tcataggtgc gcgtcgtgcc cgatgcgcgc ggtaccagcg
      481 gcagaatccg ctgccatgcc ccgtagagcg ccgggcgcat gaggtcgttc atggcggcat
      541 ccacaatggc gaaattgcgg tgaggcgtgc acttcaggtg ctccacgcgg gtgagcagca
      601 cgcccgcgtt gcccacgatg gcccggcccg gctcgatcag gatctcctgc ggccggtcgg
      661 cgagccgtgc gagaacggcg tctgcatgct cggtgggcag tggcgggttt tcatcccggt
      721 agcggatacc gaggccgccg cccagatcaa gatgctcgat cgcgatgccc tgctcggcaa
      781 gctgatcgag gagcagcagc acccggtcga gggcgtccac gaagggcgcg atgctggtga
      841 gctgcgagcc gatgtggcag tccacgccca ccacctcgat gtgcagcagc cgcgcggcct
      901 gcgtgtagat cgccggggcg gcctcgatat cgataccgaa cttgttttcc ttaaggccag
      961 tagagatgta cggatgggtg cccgcatcga catcggggtt gacgcgcaat gcgatcggcg
     1021 cgcgcctgcc gagttcgccc gcaacggcgt tgagccggtg cagttccgcc tcggattcca
     1081 cgttgaagca gcggatgccc acctccagcg cccggcgtat ctcatcggtc cgtttgccga
     1141 cgccggagaa gaccactttc gcgggatcgc cacccgcccg cagcacccgc tccaactcgc
     1201 ctactgagac gatatcgaag cctgcgccca agcgtgtcag cgtgttgagc accgcgaggt
     1261 tgctgttggc cttcaccgca tagcagacca agtgcctgcg gctggcgagc gcattgtcga
     1321 acgcgcgcca gtgatgctcg atggtggcgc gggaatagac gtagcatggc gtgccgtact
     1381 cggctgcaac ggtggcgaga tcgacctctt cggcaaacag gcgtccgtca cggtaatgga
     1441 aatgatccat ggagaaatat tccgctcgaa acgagtagct ggataaatga ccggcgtgtg
     1501 gctcgggtca ggccaagccc cattagcctt gggaggagtg ctgttgtttc ttcggcggtt
     1561 ggtccgggag gtaaaggtcg cccttctgcc cgcagccgga cagcatcgcg ccggtaccga
     1621 gtaccgcgat cacgaaccac aacagatatt gtgcccagca gggtttcacg cgcgtcccgc
     1681 tcctctaaaa attgcagtat aaaccgctgg gctgcatgct gcctatcagg tggaaggcgc
     1741 ggctggcagc tgcgagcggg tcgcgttctg gtcgagatcc tgtccctgct ggcaggcctg
     1801 cggcgtctct ggcggcgccg cgtgttgttg ggcagcacgg ggggctctcg ggtgttgtgc
     1861 tgttggccgt tgcgctgctg ctggtcgcgt tggcggtcaa tctgcagacc tatcaacgcc
     1921 tgatgtccga gcaggaggtg gccgagttgg cgtttcaggc gtggggctca caacattatc
     1981 aagtccatct gcaatatccg tcgggccaga tgctgttgtc gatctgcgcg gcgacgagtg
     2041 gcaactggac acgcacattt tgaagtggca tgggggtcgc gtgctgcccg gacttgatat
     2101 catgtaccgg ctggagcggt tgagcgggcg ctatcgcaat gtggaacagg aacagcgtat
     2161 ggatcgcagc gtttaggcat tggcgcgtga tccgggcctg gacctgtgga ccgtagcgca
     2221 gcgcccgggc cgctggattc cctggattga tacaatctat ggtggtgcag ctacatatcc
     2281 atggccgacc gcgcacagtt acactgtgag catgatcgtc tcgggccttg tagcgcgccc
     2341 tggcaacccc gtggcctggg cggcggtagg cgagtggcgt tgattgcagc gaaggtgagc
     2401 cttgcggctt aagaccaata tctttctgtg ggtctctctg gcgaccgtcc tgccgttgac
     2461 gttcctggtg ctcggcgcga ccgcctacag cgagcgcatc taccgcgaaa aagtggtcgg
     2521 cgaggtcaac gccagcctca acaacgtcat tgccgagatc gaccggcggt tgcgggtcga
     2581 gcgggagatg gtgctggcgc tcaccgaggc gccggcgatg cgccgcttct ttcctgtgct
     2641 ggaaagggcg gcagtcggtg aggtccatgc agaattttcc cggcgtagcg acgagttcaa
     2701 cgcctttctg gaggcctttc agaacatcgt gccgagccta tcc
//
LOCUS       DASUTG010000501         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2003270, whole genome shotgun sequence.
ACCESSION   DASUTG010000501 DASUTG010000000
VERSION     DASUTG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003270"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF089_11475"
     CDS             <1..404
                     /locus_tag="VF089_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174625.1"
                     /translation="GIYQVAEGYPTLLTEAQANLEMRNWDEVSVKAIAAALPALAESV
                     DFIVVGNNAGQGLPLAQSLPPNVIAEHAAVVYAEGLPQMGRYRKIGFRKFFRRSEAAG
                     RLVELATKTQRPLELLFINTIQHNETNYHDP"
     gene            571..2475
                     /locus_tag="VF089_11480"
     CDS             571..2475
                     /locus_tag="VF089_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6174626.1"
                     /translation="MPLLRLENISLSYGYLPLLAQVQFQIEAGERVCLVGRNGTGKTT
                     LFRVISGEAVPETGEVWAKDGLRVAHLEQDVPADEEQTVFQVVASGLGELGRLLFEYH
                     QVTRQVGEAEPTSLESLATLNARIESLGGWNLNQKVDTVLTRLGLIADKPLAACSGGV
                     RRQVLLAKALVCEPDLLLLDEPTNHLDISTITWLEKFLLAFRGALMFITHDRSFLRHL
                     ATRIVELDRGKLSSYPGDFDAYLRKKDELLVIEERAAAKFDKKLAEEEAWVRRGIKAR
                     RTRNEGRVQALYELRRERSRRTDGLGKANFEIDAGALSGKLVVDVRRVSFGYGDELVV
                     RDMSTRILRGDRVGIIGPNGSGKSTLLKLILGEIKPTSGEVVLGTRLQLAYFDQHRRI
                     LDPDKTVRENLSDSDHVSVQGRSRHVIGYLKDFLFPPQRIDSPVKALSGGERNRLLLA
                     KIFTQPANMMVLDEPTNDLDVDTLELLEDLLADYRGTLLLVSHDRTFLDNIVTSTLVY
                     EGSGKFVEYAGGYSDWVRCQELSQAKYAKQPNRSSMAVIARADRPIKLNSKLSYKEKR
                     ELEALPARIEALETEQSELQLRMAQADFYRQPGATITSALQRLEALGNELEECYARWQ
                     SLESQLSAGG"
     gene            complement(2509..>3154)
                     /locus_tag="VF089_11485"
     CDS             complement(2509..>3154)
                     /locus_tag="VF089_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX6174627.1"
                     /translation="VVTIQADCEIGGPHLYVKDILPSAPTKTDQLLVARTATEELDHY
                     RKIARVAGDFGVDVSFVLSWPNQKRYLEAFRGTINTWEDFAVFGFLIDRVGRYQLEEF
                     FGCTYQPLNEILPTIIKEEIGHVGYGESKTAELAARGEESREKVQAALDRWYVKALDM
                     FGRSDSTRDQRYIYWGLKRRTNTQARQEYIREVDPLIEKIGLRVPDPIDGRQYL"
BASE COUNT          729 a          823 c          860 g          742 t
ORIGIN      
        1 gcgggatcta tcaagttgcc gaaggctatc caactctact gaccgaggca caagccaacc
       61 tggagatgag aaattgggat gaggtgtccg taaaagcaat tgccgctgca ttgccggcgc
      121 ttgctgaaag cgtcgatttc atcgtcgttg gcaataatgc agggcagggc ttgccgctgg
      181 cgcagagttt accccctaac gtgatcgccg aacatgccgc cgtggtttat gctgaaggcc
      241 tgccgcagat gggccggtac cggaagattg gctttcgcaa attttttcgc aggagcgagg
      301 ctgccgggcg gcttgtcgag ctggcaacga agactcagcg tcctctagag ctcttattca
      361 tcaacaccat tcaacataat gagacgaact accacgatcc gtgatgccga accaagctat
      421 cgtaaggcct tacctgcaaa taacttaaat agcgctgcac cagaaacaga gacaagcctt
      481 cgatctagcg atgctggttc gttagagacc gagcgctttt gactccctcg cagaactgtt
      541 actttggtaa attgcgctga gtcaacgacc atgcctttat tacgcctaga aaatatctcg
      601 ctgtcttatg gctatttgcc attgctcgct caagtacagt ttcagattga ggccggagag
      661 cgggtttgtc ttgtcggtcg aaacggaacc gggaagacaa cgttgttccg cgtcatcagc
      721 ggtgaggctg tacccgaaac aggcgaggtc tgggccaagg atggtttacg ggttgcgcat
      781 ctagagcaag acgtgccggc ggacgaggaa cagacggttt ttcaggtcgt tgcttcaggt
      841 ttgggcgaac tcgggagact cctattcgaa tatcatcagg tgacgcggca ggttggtgag
      901 gccgaaccaa catcgctgga gtcgttagca acactaaatg cgcggatcga atcgttgggc
      961 ggctggaacc tgaaccagaa ggttgacacg gtattgacgc ggctcggtct gattgctgac
     1021 aagccgctgg cggcttgttc cggcggcgtc cgtcgccaag tattgctggc gaaggcattg
     1081 gtttgtgagc ccgatctgct tttgctcgat gaacccacta accacctcga tatcagcacg
     1141 attacctggc tcgagaagtt tctcttagct tttcgcggcg cattaatgtt tatcactcac
     1201 gaccgcagct ttcttcgtca cctggctacg cgcatcgtcg agcttgaccg cggcaagttg
     1261 agttcgtatc ccggcgactt cgacgcctac ctgcggaaaa aggacgagct tctcgtgatt
     1321 gaggagcgag cagccgcaaa atttgacaaa aaactcgccg aggaagaagc ctgggttaga
     1381 cgcggtatca aagcacgccg gacgcgcaat gaaggacgcg tgcaggctct atacgagctg
     1441 agacgggagc gtagccggcg tacggacggc ctgggtaagg ccaattttga aatcgatgcc
     1501 ggcgccctat ccggaaagct ggtggtggat gtgcggcgtg tgagtttcgg ctacggcgat
     1561 gagctggtag ttcgggacat gtcaacacgt attttacgtg gtgatcgagt cggaatcatc
     1621 gggcccaacg gttccggcaa aagtacgctc ctcaagctga ttctgggtga gatcaagccg
     1681 accagcggag aagtagtctt gggcacgcgc ctccaactgg cttacttcga tcagcatcgg
     1741 cgtattctcg atcccgacaa gaccgtgcgc gaaaatctca gcgactccga tcacgtttcg
     1801 gttcaaggcc gttcacgtca tgttatcgga tatctgaaag attttctctt cccgccgcaa
     1861 cgcatcgact cgccggtcaa agctctctcc ggtggtgaac gcaaccggct actgctggcc
     1921 aaaatcttta cgcagcccgc caacatgatg gtgctcgatg agccgacgaa cgatctcgat
     1981 gtcgatacgc tggaattgtt ggaggatctg ctagcggatt acagaggaac actgttgctt
     2041 gtaagccatg atcgaacatt tctcgataat atcgtcacca gcacattggt ctatgaagga
     2101 tctggaaagt tcgtcgaata tgccggcggt tattcggact gggttcgttg tcaggaactg
     2161 agtcaggcaa agtatgctaa acagccgaac cgaagttcta tggccgtaat tgctcgagcc
     2221 gatcggccga tcaaactcaa cagtaaatta tcgtacaagg aaaagcggga actcgaagcc
     2281 ctgccggcca gaattgaagc gctggagaca gagcagtcag aattacagct tcgtatggca
     2341 caagctgatt tttaccgcca gcccggcgcg acaatcactt ccgctctcca gcgactcgag
     2401 gcgctcggga atgaattaga ggaatgctac gctcgatggc agtccttaga atcgcagctg
     2461 agcgccggcg gatgactgaa cccctccgtg tgatcatact ctgcttcatc agagatactg
     2521 tcgaccgtcg ataggatcag ggactctcaa tcctattttc tcaatcagcg gatcaacctc
     2581 gcggatgtat tcttgccgtg cttgagtgtt ggttctgcgt ttcaagcccc aatagatata
     2641 tcgctggtcg cgtgtggagt ccgatcggcc gaacatatcc agcgctttga cgtaccatct
     2701 atccagtgcc gcctggactt tctctctcga ttcttctcct ctggccgcga gttcggctgt
     2761 tttgctttcg ccataaccaa catggccaat ctcttctttg atgatggtcg gcagaatctc
     2821 gtttagcggc tgataggtgc agccaaagaa ctcttcgagt tggtaacgtc caacccgatc
     2881 gattagaaat ccgaagaccg cgaaatcctc ccaggtgttg atggttccgc ggaatgcctc
     2941 cagataacgc ttctgatttg gccagcttaa tacgaatgaa acatcgacgc cgaaatctcc
     3001 ggctacccgc gcaatcttgc gataatggtc tagctcttca gttgcagttc gcgctactaa
     3061 taattgatcc gtcttggtcg gcgctgacgg cagaatatcc tttacataaa gatgcgggcc
     3121 gccaatctca caatccgctt ggattgtcac cacg
//
LOCUS       DASUTG010000502        10418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1261703, whole genome shotgun sequence.
ACCESSION   DASUTG010000502 DASUTG010000000
VERSION     DASUTG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261703"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VF089_11490"
     CDS             <1..1050
                     /locus_tag="VF089_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6174628.1"
                     /translation="KTKEAVRPLIPSELAVENPLECGVAGFGDEQGFINIVKLHAEDD
                     GVDLLAIHGELPRYPEKREATLFKNLAAATDKPILAFARSTYSCTDESRTFQEQAGIP
                     FLQAIKPTLRALAGLGLYGDRKRLGVPTLPDANGKPEDLTGDKLNALLQDRGIGLPKQ
                     SLVGTAAEAGLKAKEIGFPVALKLVAAEIVHKTESGAVVLGLKTAEEVVAEGQKLLSK
                     TLGKGHLLIQELVQGTEVLLGARTDPQYGPFLIVGLGGILVEVLKDVAIRLLPVDEGE
                     ARRMLQELRGFKVLQGVRGQGPRDIDALVKAMVGLSDIFAAQRNYLTDLEINPLMVRP
                     QGRGVAAVDVRLVRK"
     gene            complement(1056..1682)
                     /locus_tag="VF089_11495"
     CDS             complement(1056..1682)
                     /locus_tag="VF089_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6174629.1"
                     /translation="MRPEKFLTEAEQERVHQAVIAAESKTSGEIVPMVVTSSARYTEI
                     ELFGIVGGLFLGMLLSWLWQDPWGSYRLNLWPVIGATLGFLIARIPAVKRRLASRSRV
                     EEAVNDRCMAAFTEHGLHYTRDHTGILILVSLLEHRVEVLADRGINEKVPPGTWNEIV
                     EILTAGLKSRHAGDAFCKAVERCGDILATHFPRQADDRDELPNKLVND"
     gene            complement(1686..2504)
                     /locus_tag="VF089_11500"
     CDS             complement(1686..2504)
                     /locus_tag="VF089_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX6174630.1"
                     /translation="MREYPHRRIPPRSHFSAPHGLTLGIFLLCLLSSQAYPLDVPPLR
                     GRVNDYAKVITADQTRKLEDQLAQFERDTGHQVAILTVPSLEGDSLEDFSIRVAENWK
                     IGKKGFDNGVILIVVPKDRRLRLEVGYGLEGVLPDAIANQIISQYIIPHFRAGDFSSG
                     IIAGIDAVLRVIKKEPLPEAARQKPSNPSGSGFGSLTGLVLLLGLIAVMSIVSNSNRR
                     RQGIWGTRGRRHPPIFWGGGGFGRGGFGGGGGFGGGGFGGGGGGFGGGGASGSW"
     gene            complement(2525..3121)
                     /locus_tag="VF089_11505"
     CDS             complement(2525..3121)
                     /locus_tag="VF089_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6174631.1"
                     /translation="MRIQTSRWYFLRPYMVVLMLTATGCGYNTLQSLDEEVKGAWGEV
                     QNQYQRRADLVPNLVATVKGAAKFEQDTLTQVIEARSNATATKIDPESLNNPEAFKKF
                     EQAQSQLSSALSRLLVVVERYPELKANQNFQNLQAQLEGTENRIAVARKRYVERVAQY
                     NTSVRSFPTNLTAKFLGLEVRPSFSAEEAAQKPPQVKF"
     gene            complement(3280..4128)
                     /locus_tag="VF089_11510"
     CDS             complement(3280..4128)
                     /locus_tag="VF089_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174632.1"
                     /translation="MADQSAEELLLDVRVAEASKLYKFYLNNEQKILGTASVVTFLVA
                     WELVGNVLQLINPMFMSAPSLIFKAAVDMFSSGEIYNDLYVSGLELFWGYLLSAIVAV
                     PFGIMVGWYKRMSYIFDPFINAMNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFPI
                     LINTRDGVKTTPYNLLTAARSFGASEWMLFKTVVLPSTIPFILTGMRLGLGRAIVGVM
                     VGELYAATAGIGFMITVAGATFQTDKVFVGVLIFALTGMIGMEALTRIERRFDKWRPK
                     VGAQEI"
     gene            complement(4121..4582)
                     /locus_tag="VF089_11515"
     CDS             complement(4121..4582)
                     /locus_tag="VF089_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6174633.1"
                     /translation="MAELRYQIRKFLRFSENAARQAGIEPQQHQLLLAIRGLPDGASP
                     TIGVLAERMQLQHHSTVELVDRLVDRNFLCRLRSTNDRRQVLVKLTHDGEEFLQKLSL
                     HHLEELQSAGPTFVKVLNSLLGDNLTDKSDSASKSVKENVTHKPRKDRDHG"
     gene            4793..5107
                     /gene="rplU"
                     /locus_tag="VF089_11520"
     CDS             4793..5107
                     /gene="rplU"
                     /locus_tag="VF089_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6174634.1"
                     /translation="MKYAVIKTGGKQYRVSEGDLVKIEKLTGEVGEKITLADVLFIGG
                     NGEVKIGAPLVENASVTGEIISQAKAKKVLVFKKKRRKSYSRQRGHRQHQTTLKITGI
                     EG"
     gene            5123..5380
                     /gene="rpmA"
                     /locus_tag="VF089_11525"
     CDS             5123..5380
                     /gene="rpmA"
                     /locus_tag="VF089_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6174635.1"
                     /translation="MAHKKAGGSSRNGRDSKGQRRGVKVFGGETVRAGNIIIRQLGTR
                     IHPGRNVGMGRDFTLFALVDGVVNFDRLDRSRKRVNVLPAQ"
     gene            5553..6596
                     /gene="obgE"
                     /locus_tag="VF089_11530"
     CDS             5553..6596
                     /gene="obgE"
                     /locus_tag="VF089_11530"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6174636.1"
                     /translation="MKFIDETKIRVIGGDGGRGCVSFHREKFISRGGPDGGNGGAGGD
                     VIAVADPQLTTLLDLRYHKECKAGRGQHGMGSNCHGRRGEDFIVKVPVGTIVRDARTG
                     ETIGDLDKAGDRLVLAAGGRGGKGNAHFVSSTNRSPRFAQPGEPGEDRELEIELRLLA
                     DVGIIGVPNAGKSTLISVISAVRPKIADYPFTTLVPNLGVVSYDEGKSFVVADIPGLI
                     EGAHRGHGLGDKFLRHVTRTSVLIHLLDASRTDPKNPLADWSTINRELALFDEELGTK
                     PQIVVANKIDIPEARENAKVLEERLPESFRPLHLISAATGEGVKALVQKVGIHLERAK
                     HVKEEEAGDAARS"
     gene            6577..7713
                     /gene="proB"
                     /locus_tag="VF089_11535"
     CDS             6577..7713
                     /gene="proB"
                     /locus_tag="VF089_11535"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX6174637.1"
                     /translation="MTQRDHKKSILRRARRVVVKIGSQILSSSTGIEEARLEGLVSEL
                     AALHDQGKEIVVVSSGAVAAGMARLGRRERPQSIPEKQALAAVGQIRLMALYERAFGK
                     FSKNVAQVLLTHEDLANRQRYLNAKHTFQMLLASSIISIVNENDTVAVDEMKFGDNDH
                     LSALVATLLEADLLLVLSDVEGVYDRDPRLDSDAVIVPLIQDIKDIKSKVIGPSRSVF
                     GTGGMATKLAAAEEAAVAGIPTVITSGFMAGSIPRVFDPKEEIGTLILPDSNRLTNRK
                     HWIAFNLKPAGEVVVDSGAHEALVRKKTSLLPSGVREVRGSFGVGECVRCLDLDGREF
                     ARGLINYSALELNQIKGLHTSRIEQALGYKPFDEVIHRDDLVLL"
     gene            7725..8990
                     /locus_tag="VF089_11540"
     CDS             7725..8990
                     /locus_tag="VF089_11540"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX6174638.1"
                     /translation="MANASYAEQAIQLGKQAKAAARRLAVLSSDDKNRALQLMADRLE
                     DQSAFLIEENQNDLDSAKKAGIAGAVLDRIALTRSRVQAMAKGLREIAILPDPVREIL
                     KISRRPNGLQVGKMRIPLGVIGIIYEARPNVTADAAALCLKSGNAVILRGGSEAHHSN
                     QAIGKVLQAACSETAVPQHAIQVVESTDRGLVHELLQLESYIDLIIPRGGEELIRAVV
                     ANSKVPVIKHYKGVCHIYVDVDAELEMAERICLNAKVQRPSVCNAMETLLVHEAVASQ
                     FLPPLIAKYQAAGVEIRGCHRTCAVVPGIKKANEEDWTTEYLDLILAVRVVKDIDQAI
                     AHIEQYGSEHTESIITTDYRRSREFIDRVNSSAVMVNASTRFNDGGELGLGAEIGIST
                     SKIHAFGPMGLEELTTTKFIVFGDGQIRE"
     gene            9155..9805
                     /gene="nadD"
                     /locus_tag="VF089_11545"
     CDS             9155..9805
                     /gene="nadD"
                     /locus_tag="VF089_11545"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6174639.1"
                     /translation="MRIGLFGGTFDPIHWGHLRSAEEVKEAFDLDRVFFIPASVPPHK
                     RGQTRTPARDRLEMVRLAVAKNPRFTVSTVEIARPGVSYSIDTIRHFSENECKGASLY
                     FIIGLDAFAEIGTWKDFEKIFPLCNFIVTSRPGNGYASPLRGTSIAVRRLFCYDFKRK
                     IYTHRCGTSLHFLKLTDFAVSASDIRERVRHGKSIRYLLPFSVEAYIKNHGLYKKK"
     gene            9802..10215
                     /gene="rsfS"
                     /locus_tag="VF089_11550"
     CDS             9802..10215
                     /gene="rsfS"
                     /locus_tag="VF089_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6174640.1"
                     /translation="MNKPTEKSAIERALTLVGAALDKKASQLVLLEVRDFTSIADYFI
                     ICSGRSDRQVQSIAQGIQEAAREAEIAAISIEGTSRGHWVLMDFADVIVHVFYQPVRE
                     FYDLDGLWADAPKVQLPEPYSTLVHQFQIAAEQHP"
     gene            10274..>10418
                     /locus_tag="VF089_11555"
     CDS             10274..>10418
                     /locus_tag="VF089_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174641.1"
                     /translation="MKGTIVATNQPVILVVLDGFGINPKKEGNAIANASMPNMDSLLR
                     KYPT"
BASE COUNT         2424 a         2727 c         2943 g         2324 t
ORIGIN      
        1 aagaccaaag aggccgtgcg cccgctgatc ccgtcggagc tggctgtcga aaatcctctc
       61 gaatgcggcg ttgccggttt tggcgatgag cagggcttca tcaatatcgt taagctgcat
      121 gccgaggacg acggtgtcga tttgcttgcg attcacggcg agttgccgcg gtatcctgag
      181 aaaagagagg ccaccttgtt caaaaatctt gcggccgcaa ctgacaagcc gatcctcgcc
      241 tttgcgcgct ctacctacag ttgtaccgac gagagccgaa cttttcagga gcaagctggg
      301 ataccctttc tgcaggcgat caagccgaca ttgcgcgccc tcgcgggctt gggtttatac
      361 ggcgaccgca aacggctcgg tgtgccgact ctgccggatg ccaacggcaa accagaagac
      421 ctgacgggcg acaaactcaa tgccctgttg caggaccgcg gcatcggctt gccaaaacaa
      481 tcccttgtag gaaccgcggc cgaagctgga ttgaaggcaa aagagattgg ctttccggtg
      541 gcgttgaagt tggtcgcggc ggaaatcgtc cataagaccg aatccggtgc ggtggtactg
      601 ggtcttaaga ccgccgagga agtggtagct gaaggacaga agcttctatc taagacgctg
      661 ggcaaaggcc atctgcttat tcaggaattg gtgcagggca cggaggtgct gttgggagcg
      721 cgcacggatc cgcaatacgg tccgtttttg atcgtcggac tcggtggcat tctcgtcgaa
      781 gtgctgaaag atgttgccat ccgcctgctg ccggtcgatg agggagaagc gcgccggatg
      841 ttgcaagagc tgcgcggctt caaagtgctt cagggagttc gagggcaagg cccgcgcgat
      901 atcgacgctc tggtgaaagc gatggttggc ttgtcggata tttttgccgc ccagcgcaat
      961 tatctaacgg atttagagat caatcctctg atggtccggc cgcagggtcg gggcgtagcc
     1021 gcggtggacg tgcgcttggt gcggaagtaa gattcttaat cattaaccag tttgttgggc
     1081 agttcgtcgc ggtcgtccgc ctggcgcgga aagtgagttg ctagaatatc gccgcaccgc
     1141 tccacagctt tgcagaaagc atcgccagca tggcgtgact tcaaacccgc ggtgaggatc
     1201 tcgacgattt cattccatgt gccgggtgga actttttcgt tgattccgcg gtccgcgaga
     1261 acttcgactc gatgctccaa taaagacacc agtattaata tgccggtatg gtcgcgtgtg
     1321 taatgcaggc cgtgctcggt gaatgcagcc atacaacgat cgtttacagc ttcctcgact
     1381 cgactcctcg aagctagccg gcgtttgact gcagggatgc gggcgatcaa aaagcccaga
     1441 gttgcgccga ttaccggcca taaattaaga cgataggatc cccagggatc ctgccagagc
     1501 cagctcaaaa gcattccaag aaaaagaccg ccgacgatcc cgaacagttc gatttcagtg
     1561 taacgcgcgc tggacgtgac aaccatcggc acaatttcgc cggaagtctt ggactccgcc
     1621 gcgatcaccg cttggtgaac acgttcttgc tccgcttcag taagaaattt ctctgggcgc
     1681 atcgatcacc aactgccgga agctccgcca ccgccaaagc caccgccacc accaccgaaa
     1741 ccgccgccac caaaccctcc gccgccaccg aatccacctc tgccgaaacc accgccaccc
     1801 cagaagatcg gcggatggcg cctgccgcga gtcccccata tgccttgtct tcggcggttt
     1861 gaattagaga caatcgacat aacggctata agccccaaga gcagcaccag gcccgtgagc
     1921 gagccgaacc ctgatccgga cgggttggaa ggtttctgcc gggccgcttc cggaagcggt
     1981 tccttcttga tcactctcaa cactgcgtcg atacctgcga tgatgccgct cgagaaatca
     2041 ccggcgcgga aatgtgggat gatgtattga ctgatgatct ggttcgctat ggcatcgggc
     2101 aaaacgcctt ccaagccgta tccgacctcc aggcgcaatc ggcgatcctt cggcacgact
     2161 atcagaatga caccgttgtc gaaacctttc ttaccgattt tccaattctc ggcaacccga
     2221 atactgaagt cctcgagcga gtcaccctcc agcgaaggga ctgtcaagat cgccacctgg
     2281 tgacccgtgt cgcgctcaaa ctgcgcaagt tgatcctcca acttgcgagt ttgatccgct
     2341 gtaataactt tggcgtaatc gttcacgcgc ccgcgcagcg gcggcacatc gagcgggtag
     2401 gcctgagacg aaagtagaca aaggaggaag atgcctagcg taagcccatg cggcgcggaa
     2461 aaatgactgc gcggcggaat ccgtcgatga ggatattcgc gcaaggtatt gatcgccgaa
     2521 ccgcctagaa ttttacctgt ggcggttttt gcgcggcttc ctcggcgctg aacgacggcc
     2581 gtacttctaa gcccaaaaac tttgccgtga gattagttgg aaaagagcgc acggaagtat
     2641 tgtactgagc cactcgttcg acatagcgct tgcgtgctac cgcaatccgg ttttcggtgc
     2701 cttcaagctg tgcttgcaag ttttggaaat tttgatttgc cttaagctcc ggatatcgtt
     2761 ccaccacaac cagcagacga gataaagcgc tggagagctg gctttgcgcc tgctcgaact
     2821 ttttaaatgc ttccggattg ttgagcgatt cgggatcgat tttggttgct gtcgcgttgc
     2881 tgcgggcttc gatcacttgc gtcagagtgt cctgttcaaa cttcgctgct ccttttactg
     2941 tggcgactaa gttcggcacc agatcggcgc gacgctgata ttgattctga acttctcccc
     3001 aggcgccttt cacttcttca tccaggctct ggagggtgtt atagccgcac ccggttgcgg
     3061 ttagcattaa aacgaccata tacggacgaa gaaagtacca acgtgatgtc tgtatgcgca
     3121 tgcaaggtcc tttcgtgagt cagaagtcca gattccacaa tggcaaacgg caggagggtc
     3181 atcaagaggc tggcgaaggc aatcgaacga atgccaaaaa aaaggcccct cgtgagaggg
     3241 gccctttgac tgcgcgatcc gagaaactta cgttcctgtc tatatttcct gcgctcccac
     3301 tttcgggcgc catttgtcga agcgccgctc gatccgggta agtgcctcca taccgatcat
     3361 acctgtcaag gcgaagatca gcacaccgac aaaaacctta tcggtctgga aagtggcgcc
     3421 ggcaacggtg atcatgaaac cgatgcccgc ggtagcggcg tacagctcgc cgaccatgac
     3481 tccgacgatg gcccggccca gcccaaggcg cataccggtc agtatgaatg ggattgtcga
     3541 aggcaacacc accgtcttga acagcatcca ctccgaagcg ccgaagctcc gtgccgcggt
     3601 gagaaggtta tacggtgtgg ttttgactcc atcgcgggtg ttgatcaaga tcgggaaaac
     3661 ggctcccaaa aagatgatac cgaccttgga gagaatgccg attcccaacc agataatcac
     3721 cagaggcaaa agcgccactc gcggagtcgc gttcatggcg ttaatgaaag ggtcaaagat
     3781 gtagctcatc ctcttgtacc agccgaccat gatgccaaat gggacggcga cgatagcgga
     3841 cagaagatat ccccagaaga gctcgagccc gctgacatag agatcgttgt atatctcccc
     3901 ggaggaaaac atgtcgacag cggccttgaa gatcagggaa ggcgcgctca taaacatggg
     3961 attgatcagc tgaaggacat ttccaacgag ctcccacgcc actaagaacg tgacaacact
     4021 tgctgtgccg agtattttct gttcgttatt tagatagaac ttgtaaagtt tcgacgcttc
     4081 ggcaaccctt acgtcaagca atagttcttc agccgattga tcagccatgg tctctatcct
     4141 ttcgcggttt atgggtcacg ttttccttca ctgatttaga ggcggagtcg gatttgtccg
     4201 tgaggttatc gccgagcagg ctgttaagga ccttgacgaa agtcggtccg gctgactgaa
     4261 gttcttccaa gtggtgcagt gaaagcttct gcagaaactc ttccccatcg tgcgttagct
     4321 tgactaacac ctgccgccgg tcgttggtcg accgcaagcg gcacaagaaa tttcgatcta
     4381 ccagacggtc tacaagctct accgtgctat ggtgctggag ctgcattctt tcggctagca
     4441 ctccgattgt cggacttgcc ccatccggta atcccctgat ggccaagagc aattggtgtt
     4501 gttgcggctc tatacccgct tgccgcgcgg cattttcgct gaacctgagg aacttacgta
     4561 tctggtaacg tagttccgcc attgctttaa agttgggcac cggtcaccat ctctaatatc
     4621 gcgtcacgat actatcgtat tacgatgtat atttaacggt cgagcctttg tcaactaggt
     4681 cttggaaagt tttttgcctt cgttgtctca ggagttgcca gaggtatctt gtggccggat
     4741 attgttgact ttggccaaaa tctccgttag aaaaaaccgt tcgggaaaaa acatgaaata
     4801 tgcagtaatc aaaactggtg ggaagcagta ccgggtatct gaaggtgatc tcgtaaagat
     4861 cgagaagctt accggcgagg tcggcgaaaa gataactctg gctgatgttt tgtttattgg
     4921 cggcaatggc gaggtcaaaa tcggtgcgcc gctggtagag aacgcctctg tgacgggtga
     4981 gattatcagt caggcaaaag cgaagaaggt tctggttttc aaaaagaaaa gacgcaagag
     5041 ttacagtcgc caacgcggtc atcggcaaca tcagaccact ctcaaaatca ccggaattga
     5101 aggttaaaag gtcggaacgg atatggcaca taaaaaagca ggcggtagct ctcgcaatgg
     5161 ccgcgacagt aagggacaac ggcgcggtgt caaagtgttt ggcggcgaga cggtgcgtgc
     5221 gggtaatatt atcatccgcc aactcggcac acgcattcac cccggccgaa acgtgggcat
     5281 gggccgggac tttactttgt ttgcgttagt cgatggggtg gtgaacttcg acaggttgga
     5341 ccggtcgcgt aagcgagtaa acgtgctgcc agcgcaatag gaattcgcct gtttttactt
     5401 gaaagccccg caaggggctt tttttttgcc caagcgataa gcaatggtgc agtgagtcga
     5461 gaatgggtaa agtagatgag aagtcgtagc taaccgtgat ccgctaatct ggccgaactg
     5521 gtccggacct gcgggtcacg tataatcagg tcatgaaatt catcgatgag acaaaaatac
     5581 gggtcattgg cggcgatggc ggacgtggtt gcgtcagctt tcatcgtgag aagttcatta
     5641 gccgcggcgg acctgacggt ggcaatggtg gggccggtgg cgatgtcatc gcggttgcgg
     5701 atccacaact cacgacgttg ctggatcttc gctatcataa ggaatgcaag gccggtcgcg
     5761 gccagcacgg aatgggcagc aattgtcatg ggcgtcgcgg tgaggatttc atcgttaaag
     5821 tgccggtggg aacgatcgtt cgcgacgcca ggacgggcga gactatcggc gatctggata
     5881 aggccgggga ccgtcttgtg ctcgccgccg gcgggcgagg cggcaaaggc aatgcgcatt
     5941 tcgtttcatc gaccaaccgt agcccacggt ttgctcagcc gggtgaaccc ggtgaagatc
     6001 gcgagttgga gatcgaattg cggctcttgg ccgacgttgg aattatcggc gtccccaacg
     6061 ccggtaagtc gactttgatc tcggtcattt ccgcagtgcg gccaaagatt gccgactacc
     6121 cgtttacgac cctggtcccc aaccttggag tggtcagcta cgatgaggga aagagtttcg
     6181 tggtggccga tattcccggg ttgattgaag gcgcgcaccg gggacatgga ttgggagaca
     6241 agtttttaag gcatgtgaca cgcacgagtg tgttaattca cctgctcgat gcatcgagaa
     6301 ccgatccgaa aaatccgctg gccgactggt ccaccatcaa tcgcgaactg gcacttttcg
     6361 atgaggagtt gggcacaaag ccgcaaatcg tcgttgccaa caagatcgat attcccgagg
     6421 cacgagagaa cgcaaaggtt ctcgaagaaa gattgccgga gtcttttcgg ccactgcatc
     6481 taatctccgc cgcgaccggt gagggggtta aggcgctggt gcaaaaggta ggcatacatc
     6541 tggagcgagc caagcacgtt aaggaagaag aagctggtga cgcagcgcga tcataagaaa
     6601 tcgattttgc gccgggcgcg ccgggttgtg gtcaagatcg gcagccagat cctttcttcc
     6661 tcgaccggca tagaggaagc ccggctcgag ggtctagtct ctgagctggc agcgctgcat
     6721 gatcaaggca aagaaatcgt cgtggtcagt tccggggccg tggcagcggg catggctcga
     6781 ctcgggcgca gagagcggcc gcagagcatt cccgagaaac aggcgcttgc ggctgtcggc
     6841 caaatcagat tgatggcgct atacgagcgc gcgtttggca agttcagcaa gaacgtcgcg
     6901 caggttctgc tcacccacga agatctcgcc aaccgccaac ggtatctcaa tgccaaacat
     6961 acctttcaaa tgttgctggc atcgtcgatc atctccatcg tcaatgaaaa cgataccgtt
     7021 gccgtcgatg aaatgaaatt cggcgacaat gaccatctct cggcgctggt ggcgactctg
     7081 cttgaagccg atctgttgct tgtcttaagc gatgtcgaag gcgtctatga ccgcgatcca
     7141 cgtttagact ccgatgctgt tatcgttccg ctgattcaag acatcaaaga tatcaaatcc
     7201 aaggtgatcg gccctagccg cagtgtcttt ggcaccggcg gcatggcaac caaactcgct
     7261 gctgccgaag aagcggcggt cgccggcatt cccaccgtga tcacgagcgg gttcatggcg
     7321 ggttcgattc cgcgcgtgtt tgaccctaaa gaagaaatcg gaacgctgat acttccggat
     7381 agcaaccgct tgaccaatcg caagcattgg atcgctttca atttaaagcc cgcaggtgag
     7441 gtggtcgtcg attcgggagc tcacgaggct ctggtgcgaa agaaaaccag tctgctgcct
     7501 tcaggagtga gagaggtgcg cggctctttt ggcgtaggtg agtgcgtgcg ctgcttggat
     7561 cttgacgggc gggagtttgc ccgtggtttg atcaactaca gcgcgttgga gttgaatcaa
     7621 atcaaaggat tgcatacaag caggattgaa caagctctcg gctacaagcc atttgacgag
     7681 gttatccatc gcgacgatct ggtgttgctg tgaagaaaaa cacattggcg aatgcctcat
     7741 atgccgagca agcaatccag ctgggcaagc aagccaaggc tgcggctcgc cgcctggcgg
     7801 tgctgtcttc cgacgataag aatagggcgc tgcaactcat ggccgaccgg ctcgaggatc
     7861 aaagcgcatt tctgatcgag gagaaccaga atgatctgga ttctgccaag aaagcaggga
     7921 tcgccggcgc ggttctcgat agaattgcgc taactcgtag ccgggtacag gcgatggcca
     7981 aaggcctgcg tgaaatcgcg atcttaccgg acccggtgcg cgagatactt aagatctcgc
     8041 gccgccccaa cggcttgcaa gtcggaaaaa tgcgcattcc tttgggtgtc attgggatca
     8101 tctatgaggc ccgccccaac gtgaccgccg atgctgcggc gctctgtttg aagtctggca
     8161 acgcggttat cctaagaggt ggaagcgagg cgcatcactc gaaccaggca atcgggaaag
     8221 tactgcaagc cgcctgctcc gaaacggcgg tgccgcagca tgccatccag gtcgtggaat
     8281 ccacagatcg cggattggtt cacgagttgc tgcaattgga gtcgtacatc gatctgatca
     8341 ttccgcgggg tggcgaagaa ctgatccgcg cggttgtcgc gaactctaaa gtaccagtca
     8401 ttaaacatta caaaggcgtt tgtcatatct atgttgatgt cgacgccgag ctcgaaatgg
     8461 ccgaacgtat ttgcctgaac gccaaggttc aacggccctc agtttgtaac gccatggaaa
     8521 ctctgctggt gcacgaagcc gtcgcctccc aatttctgcc gccgcttatc gcaaaatacc
     8581 aggcggccgg cgtagagatt cgcggctgtc atagaacctg cgcggtagtc cccggcatca
     8641 aaaaggctaa cgaggaggat tggacgactg agtatctcga tctgattctt gccgtccgag
     8701 tggtcaaaga cattgaccag gcgatcgctc atatcgaaca gtatggatcc gagcacaccg
     8761 aatccatcat tacgacggat tatcgaagat ccagagagtt tattgaccgg gtcaactctt
     8821 cggccgtgat ggtcaatgct tcgactcgct ttaatgacgg cggagagctc ggacttggcg
     8881 ccgagatcgg tatcagcacg agcaagatcc atgctttcgg cccgatgggg ttagaggaac
     8941 tgacaacaac gaagtttatc gtctttgggg acgggcagat acgggaatga cagatcatga
     9001 aagtcgccgg tttacctggt tctgcatcaa ctagttccgc accaaacgct tccctggaag
     9061 tcacacagcg gtccgtccct gatcgccaaa tccttgcccg gcttgcaaag aggatttctt
     9121 cggtcggttc cgcgaaaggc gatgccgaat ttccatgagg attggcctct tcggcggcac
     9181 cttcgatccg atccattggg gccatctgcg tagcgcggag gaggtaaaag aagcctttga
     9241 tctcgatcgc gtttttttca ttcccgcttc ggttccaccg cacaagagag ggcagacccg
     9301 gactccggcg cgagatcggc tcgagatggt gcgcttggcc gtggctaaaa acccccgctt
     9361 cacggtttcc accgtggaga ttgcaaggcc aggcgtctcc tactccatcg acacgatacg
     9421 tcattttagc gaaaatgaat gcaagggtgc ctcgctttat tttattatcg gtctcgatgc
     9481 atttgccgag atcggcacct ggaaggattt tgaaaagatc ttcccccttt gtaatttcat
     9541 tgtaacgtcg cgcccaggga atggttatgc cagtccgctg cggggaactt ccattgccgt
     9601 gcgtaggctg ttttgttatg attttaagag aaaaatctac actcataggt gtggtacgtc
     9661 gctccacttt ttaaaactca ctgacttcgc cgtttccgcg tccgatattc gcgaacgggt
     9721 acggcacgga aaatcgatcc gctatctgtt gcctttcagt gtcgaggcct acatcaaaaa
     9781 tcacgggctg tacaaaaaga aatgaataag ccgacagaga aaagtgcgat tgaaagagct
     9841 ctgaccttgg ttggcgcggc attggataag aaggcatcgc aacttgtcct gttagaggtc
     9901 cgagacttca cttcgatcgc agattatttc attatttgtt caggccgatc cgacaggcag
     9961 gtccaaagca ttgcccaagg aattcaagag gccgcgcgcg aagcggagat cgcggctatt
    10021 tcgatcgagg gtacaagccg aggccattgg gtgttgatgg atttcgcgga cgtgatcgtt
    10081 catgttttct atcagcctgt gcgcgagttc tatgacctag acggactctg ggccgatgcg
    10141 ccgaaagttc agttgcccga gccttactcg actcttgttc accaatttca aatcgccgct
    10201 gagcagcatc cataactctt ccaggctcgc ccctgcctga agtcgccgcg gttggcgacc
    10261 aacagcagga gacatgaaag gaactattgt ggcgaccaat cagcccgtta ttctcgtcgt
    10321 gttggacggt tttggtatca acccaaaaaa agaaggtaac gcgatcgcca acgcatccat
    10381 gccaaacatg gactcgttgc tacggaaata tcccacct
//
LOCUS       DASUTG010000503         2817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1707680, whole genome shotgun sequence.
ACCESSION   DASUTG010000503 DASUTG010000000
VERSION     DASUTG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707680"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF089_11560"
     CDS             complement(<1..239)
                     /locus_tag="VF089_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174642.1"
                     /translation="MRVKATAISFLFLVVLFASSYALLLPGAAGTANPDLFVFPKYQY
                     GPADKVWDEIMVGIAKAEADYVRAVLRRGGVAPGY"
     gene            complement(981..2045)
                     /locus_tag="VF089_11565"
     CDS             complement(981..2045)
                     /locus_tag="VF089_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174643.1"
                     /translation="MPMIDADTHVDESEDTWNKLEATTYARYIPVTISMPPDEAKRAG
                     YNPTNSRCWVVEGRLQNRAIRDEVNHPPRVLRELQDVPGRLAHMDKMGVDVQVIFPTF
                     FIRYNTSNAEAEWALTTTYNRWLADKCAQTKGRLRWAAVLPLLQPDKAVEELRWAKAH
                     GACGIFKRGFDLDRNVTDPHFFPVYEEASALDLPLCIHTGHPLPASEWDRGFPIMYSF
                     TQLVSSGLAEKFPKLRFGLIESGASWIPYAISQLGAVKRQALRGQQTRLPRLFELEPQ
                     LFRANRLFVTIDPIDDIESLLKFGTEDNLMIGTDYSHTDISANLSALDEVRSWAEEGR
                     ISNAQASKILESNSKAFYGL"
     gene            2271..>2817
                     /locus_tag="VF089_11570"
     CDS             2271..>2817
                     /locus_tag="VF089_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6174644.1"
                     /translation="MNITNSTTDELRLMTSGAFTAAYLELLPRLEVLTKKKLLTAATS
                     IGTGETSIPNRLRRGEPVDVVIVADAVLVGFIKEGLIIAESYTPVARSAIGMAVRAGA
                     RRPDISTVDALTLTLLQAQSIAYSASVSGEYFSNELLQRLGIADQVLSKCRRIEGGER
                     VGAVVARGEAELGFQQISELLP"
BASE COUNT          619 a          800 c          743 g          655 t
ORIGIN      
        1 ttgtatcccg gtgcgacgcc gccacgacgc agaaccgcgc gcacgtagtc agcctctgcc
       61 ttagcgatcc cgaccataat ctcgtcccaa actttgtccg cggggccgta ctggtacttc
      121 gggaagacga aaagatcagg attggccgtg ccagcagcgc cgggaagcag taaggcgtag
      181 ctggaggcaa aaagaaccac caagaagaga aaggatatag cagtcgcttt aactctcatc
      241 gttaagtctc ctcctcattt tctgataccc gtgtttccct tctcctctcg cctccatccc
      301 tttccagaat gtcgtatttt cgatagtggt gctacggggc cgtttaccac ttggagtcgt
      361 tcaaagctct tcggtcgttg atcgtcttct gatgtgcccc gtgcgtcaag tgaaaatctc
      421 tcctagggag atagattacc tgatggccga cataatcatg gctgaagatc aaccgccgct
      481 ttgaagctgc catccggtcc cgtccgtcct cttttcagtg atccggttgg ggctatggct
      541 aaaggcgaca tcatcccggg cagtgactac caactcgcgc tgctcgcagc ccaacggcct
      601 tcgaacgtgg ataaccactc ctaccagtta ttgcgacaag catcggcgca tttgtattgc
      661 aacggcggag caacatcgaa caacagtaca tccagcgcat agttcgccct cagcccttcg
      721 acatttttgg cgcgcaacgc ctttgaccaa ctagcaacga gtttccgaat ctgcgcttca
      781 tcgtagtttt tagttcaatc gtcatatttt tctgctctga gaatcttcca tttataggag
      841 cttcatccat acagaggacg agccctttct cgcccaatca aagcgcgctt cggcgcctaa
      901 accatttcaa ccgatggtat gcggcagcaa ccccgagcta accagatcgg ctgagaatgc
      961 tgagcggggg ccatggtatt ctacaagcca tagaacgcct tggagttgct ctctagaatt
     1021 ttgcttgctt gggcgttgct gatcctgccc tcttccgccc aactgcggac ttcgtccaag
     1081 gcgctcaggt tggcggagat gtcagtgtga ctgtaatcgg tgccaatcat caaattatcc
     1141 tcagtgccaa atttcagcaa agattcgata tcgtcaatcg gatcgatcgt aacgaacagg
     1201 cggttggcgc gaaacaattg gggttccagt tcaaacaggc gcggcaatcg agtctgttgg
     1261 ccgcgcagcg cttgccgttt taccgctccc aactgcgaga tggcataggg aatccatgag
     1321 gcgcccgact cgattaggcc aaatcggagt ttcgggaact tctccgcaag accggagctc
     1381 accagctggg taaaggaata catgatcgga aaacctctat cccactcgct cgccggaagc
     1441 ggatggccgg tgtggatgca cagtggcagg tcgagcgcgc tggcttcttc gtaaactgga
     1501 aaaaaatgcg ggtcggtgac attcctgtcg agatcgaagc cgcgcttgaa gatgccgcag
     1561 gcgccgtgcg ctttcgccca acgcagctcc tcaaccgcct tgtccggctg aagcagcggt
     1621 agcacagccg cccatcgtag ccgccccttt gtttgcgcgc atttatcggc gagccagcga
     1681 ttgtaggtcg tggttagcgc ccattcagct tccgcgttgc tggtgttgta gcggataaag
     1741 aacgtcggaa agataacttg cacgtcgaca cccatcttat ccatgtgcgc gagccggcca
     1801 gggacatcct ggagctctcg caagactcgc ggcggatgat tgacttcatc gcggatcgcg
     1861 cgattctgca gcctgccttc cactacccaa caacggctgt tggtcggatt atagccggcg
     1921 cgtttggctt catccggcgg catcgaaatc gtcacgggaa tgtacctggc gtaggtcgta
     1981 gcctccaact tgttccaggt gtcttcagac tcgtcgacat gcgtatcggc atcaatcatt
     2041 ggcatcgggt tagctcctgt tcgtcattct aagcagtttg ttctttcggc attccataca
     2101 acgtttagtt ccggtttagg agtccaatat aattcgatcg cctgtcattt caacaactcc
     2161 atcagaatcc tttttacatc cctcccaatg gcttgatcgc tcttaagctt ggagatactg
     2221 atagggagta tgcgaggcga atacaacgct agtcgaagat tgagactgcc atgaacataa
     2281 ccaacagcac gacggatgag ctacggctca tgacctcagg cgcttttact gcggcctatc
     2341 ttgaactcct tccccgactc gaggtcctca ccaagaagaa actcctaacc gccgcaacct
     2401 ccataggaac gggggagacc tcgatcccga atcggctacg gcgcggtgaa cccgtggacg
     2461 ttgtcattgt cgcggatgcg gtactagtcg gattcataaa ggaagggctg atcattgccg
     2521 aaagttatac tccggttgca cgctcagcca tcggcatggc ggtgcgggcc ggagctcgta
     2581 ggccggacat cagcaccgtg gacgccctga cactcacact attgcaagcc caatccatcg
     2641 cctactcggc cagtgtaagc ggcgaatatt tttccaacga actgcttcag cgcttgggaa
     2701 tagccgatca ggtgttgagc aagtgccggc gcattgaagg cggtgagcga gtcggcgcgg
     2761 tggtcgcgcg gggagaggcg gaacttggct tccagcagat cagcgaactg ctgccca
//
LOCUS       DASUTG010000504         5935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1558824, whole genome shotgun sequence.
ACCESSION   DASUTG010000504 DASUTG010000000
VERSION     DASUTG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558824"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF089_11575"
     CDS             <1..227
                     /locus_tag="VF089_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6174645.1"
                     /translation="IDLKDGRKIEGWADERYRGGPENPLSDTDLETKVRSCCEGVLDE
                     KSQATLIDAGWSIGKMQDVGRLMEIVNKLP"
     gene            290..673
                     /locus_tag="VF089_11580"
     CDS             290..673
                     /locus_tag="VF089_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6174646.1"
                     /translation="MLDPIEAIKEKMIMVCKILQKQRVIDGYGHVTARLSNGRILSTP
                     HMPPGKVAVRDLIIIDAEGKKLEGAGEPNGETSMHTSIYKARPDVQSILHYHADELIA
                     VAASGQGIKVLSNAGGTLLSGHADL"
     gene            633..971
                     /locus_tag="VF089_11585"
     CDS             633..971
                     /locus_tag="VF089_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6174647.1"
                     /translation="MPGVHFYRGTPIYDSPLLIRNHKLGDKVAATLGDRSAVLLRGHG
                     GTVVAKNLDQLVRRGLDLVRSAKLQIMASALGQLKTHSDEECEQMIKGHEGGDAQRRF
                     LDYYISEVVD"
     gene            1234..3021
                     /locus_tag="VF089_11590"
     CDS             1234..3021
                     /locus_tag="VF089_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6174648.1"
                     /translation="MSESSKSDKSAIKKSTQRWQSDVIVDLIKQYDFPYIALNPGASY
                     RGLHDSLVNYGENDPPMLLCNHEKIAVQIAHGYAKATGKPMMAIVHNVVGLLHAPMGI
                     YYAYIDRCPVFIVGATGPMDEKLRRPFIDWIHTAFAQGDVVRGFTKWDYQPGSIHGVP
                     DSFARAYSIMMSEPQGPVYMVYDSAMQESPMKEEMALPKFKVKVPSRVAPEPAIIEEA
                     ADMLMAAENPLLLTEYAARMPIGFDSTVELAETLGASVYDINKRLGFPNRHPLSVSFH
                     GDAFKGVDVVAALDVIDWTRGSHKLNTQTREISVVTAPDCKWIDVGFADIEISKWATD
                     YNKHHNWDLRVLGDSALSLPALTTACRKRIDGNKSLTAKIDERKKAIGERHARQWRKW
                     QEQVKEEWNERPMTESRLAHEVWQVIQNEDWVLTANTLKEWVHKTWNFDRSYRHVGRE
                     LGTGTQIGMSIGVALAHKGSGKLVIDLQPDGDLMFDLGALWMPIKYQIPMLIVMYNNR
                     AYYNDWAHQIHMAHQRGTDPARAYIGMDLEGPPPDFAHIAKGIGWHAEGPIEDPAEVG
                     PAVKRAIAQVKSGKPALVDTIVWRRGENA"
     gene            3146..4933
                     /locus_tag="VF089_11595"
     CDS             3146..4933
                     /locus_tag="VF089_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6174649.1"
                     /translation="MAKPAPKAEIIKKSDKRWQSDVIVDMIKRYDFEYIALNPGASYR
                     GLHDSLVNYGENDPPMLLCQHEKIAVQIAHGYARVKGKPMIAIAHNLVGLLHTPMGVY
                     YAYLDRAPVFLIGATGPLAEPKRRPFIDWIHTANVQGEAIRNFVKWDYQPSTVDGVPG
                     AFARAYSVMMSARQGPVYMCYDAGLQEAELDHDVEMPPPGAIDVPTAPSADPAALEKA
                     ADTLAAANRVAIIADFAARPPHGWNHIVELAETLGASVWDVGSRLNFPTNHPLNLTMD
                     PEGCYKDVDVVLTLDIADFEKPTHIRDISTRTVVSKVQEKATWIDIGFTDIEISKWSM
                     DYARPFYAQQRMIADPVTTVPLLTKLLKARIGNTKGLSEKIARRNEEIGKRHAENRAK
                     WSKQAQEHWDAKPMTVPRLALEVWEAIKGDDWVLTTGDLGSWGKKLWDFDRPYCHGGR
                     ELGTGTQIGLSLGVALANKGTGRLVVDLQPDGDLMFDAGALWIAAKYKIPMLIVMFNN
                     RAYYNDWNHQIVMAKNRGTDPSRAHIGMDLYDPDPDFAGLARSMGWYAEGPFETGDDL
                     KPALKRAIEQVKQGKPALIDAVCDRRNHG"
     gene            5137..5787
                     /locus_tag="VF089_11600"
     CDS             5137..5787
                     /locus_tag="VF089_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX6174650.1"
                     /translation="MKAFSAGCALMMLVALLASPAIGAERSTAYNWTGFYAGVNGGGG
                     WGQSRHDFEAAGTTTGDYRISGATAGGTLGANLQAGGLLLGVEGDMNWSDIGGSDSCP
                     NANFTCRTRSKWLATTRGRVGYAIDRVLPYITAGAAFGRIRAQIPDFGSERETEVGWT
                     AGGGLEAGIFGGLSAKLEYLYVDFGTFNCGFACTDTLVDKVKFNAHMFRVGLNYRF"
BASE COUNT         1415 a         1567 c         1739 g         1214 t
ORIGIN      
        1 ggatcgatct caaagatgga cgtaagatcg aaggctgggc cgatgagcgc taccgcggcg
       61 gaccggaaaa cccactgagc gataccgatc tggaaacaaa agtgcgctcc tgctgtgaag
      121 gtgtcctgga tgagaagagt caggcgactt tgatcgacgc gggctggtcg atcggcaaga
      181 tgcaggatgt tgggcgattg atggagattg tcaataaact gccgtgaaca acaaacgtcg
      241 tcgttatcaa gttggaaggc tgctggtcca agacgatgaa ggagaaacta tgctcgaccc
      301 gattgaagca ataaaagaaa aaatgattat ggtttgcaag attctgcaga aacaaagagt
      361 catcgatggt tacggtcatg ttacggcgcg tctgtccaat ggacgtatac tgagcacgcc
      421 gcatatgccg ccgggaaaag tcgcggtgcg cgacctgatc atcatcgacg ctgaaggcaa
      481 aaaactcgaa ggcgccggcg aacccaacgg tgaaacgtcg atgcatactt cgatctataa
      541 ggctcgaccc gatgtccaat cgattctcca ttatcatgcc gatgagctta tcgcggtggc
      601 ggccagcgga cagggaatca aggtcttgtc caatgccggg ggtacacttt tatcggggca
      661 cgccgatcta tgattcgcca ctgttaattc gcaatcataa attaggcgac aaagtggcgg
      721 cgactctcgg cgaccgcagc gccgtgttat tgcgcggcca tggcggcacg gtggtggcga
      781 aaaatctcga tcagctggtt cgccgcgggc tggacctggt gcgctccgca aaactccaga
      841 tcatggcgtc ggccctcggc cagctcaaaa cccacagcga cgaagaatgc gagcagatga
      901 tcaaggggca cgagggaggc gatgcccagc ggcgctttct cgactactac atttccgagg
      961 ttgtcgattg aggcgaccgg caccaggctc tagcttgtca aagaaagata tgttctggca
     1021 tccgccgcaa gcatcgaagc gcggaggaaa aacccgtttg cggacgctat tggacaggtc
     1081 atgtgggctt gcaggttcat cgtttcccag ataacaaaga tgttttccgg cccactatga
     1141 ttgcggatgt aagtccatct gcaatcaaga gaccttggga cctgacaaac tgcgttagta
     1201 gaatcgttac gttgagcaga aggagaatca tgcatgagtg aatcatcgaa atcagacaag
     1261 agcgctatca aaaaatctac ccagcgctgg caatccgacg ttatcgtcga tctgatcaaa
     1321 caatacgatt tcccctatat cgcgctcaat ccgggggcga gctaccgcgg tttacatgat
     1381 tcgctggtca attatggcga gaacgatccg ccgatgctgc tctgcaatca cgaaaaaatt
     1441 gccgtgcaga tcgcccatgg ctatgccaag gcaaccggca agccgatgat ggcgatcgtt
     1501 cacaacgtcg tcggtctttt gcatgcgccg atgggaattt attacgccta catcgatcgc
     1561 tgtccggtct tcatcgtcgg cgccaccgga ccgatggatg agaaactgcg ccggccgttt
     1621 atcgactgga ttcacaccgc gttcgcgcaa ggcgatgtcg tgcgtggatt taccaagtgg
     1681 gactaccagc ccggcagcat ccatggcgtg ccggattcgt ttgcgcgcgc ctattcgatc
     1741 atgatgagcg agccgcaggg acccgtctac atggtctatg attcggcgat gcaggaaagc
     1801 ccgatgaaag aggagatggc gctgccgaaa ttcaaagtga aagtgccatc gcgagtcgct
     1861 cccgaaccgg caatcatcga agaagccgcg gacatgctga tggcggcgga gaatccatta
     1921 ttgctcacag aatacgccgc gcgcatgccc atcggatttg attcaacagt cgaactggcg
     1981 gaaacactgg gcgcttcggt gtacgatatc aataaacgcc tgggttttcc gaaccgccat
     2041 cccttgagcg tgagctttca tggcgacgcc ttcaaaggtg tcgatgtggt cgccgcgctc
     2101 gatgtcatcg actggacgcg cggctcgcac aagctcaaca ctcagacacg cgagatcagc
     2161 gtagtgacgg cgccggattg caaatggatc gacgtcggct tcgccgatat cgagatcagc
     2221 aagtgggcga ctgactacaa caagcaccac aactgggatc tacgagtgct cggcgacagc
     2281 gcgctctcgc tgccggcatt gacaaccgct tgccgaaaac ggatcgacgg caataagtca
     2341 ctgacggcaa aaatcgatga gcgcaaaaag gccatcggcg agagacacgc gcgtcagtgg
     2401 cgaaagtggc aggaacaggt gaaggaggag tggaacgagc ggcccatgac cgaatcgcgc
     2461 ttggcgcatg aggtatggca agtgatccag aatgaggact gggtgctcac cgccaatacg
     2521 ctcaaagagt gggtccacaa gacatggaac ttcgaccggt cgtaccgcca cgtcggccgc
     2581 gagcttggca ccggtactca gatcggcatg tcgatcggtg ttgctctggc gcacaagggc
     2641 tcaggcaaac tcgtcatcga cctgcaacct gacggcgact tgatgttcga tctgggcgcg
     2701 ctttggatgc cgatcaagta tcagattccg atgctgatcg ttatgtataa taaccgcgct
     2761 tactacaacg attgggcgca tcagattcat atggcgcatc agcgtggcac cgatcccgcg
     2821 cgcgcatata tcggcatgga tctcgaaggc ccgccgccgg attttgcgca catagccaag
     2881 ggcattggct ggcatgccga ggggcccatc gaagatccgg ccgaagtcgg cccggcagtg
     2941 aagcgggcaa tcgcacaggt caagagcggt aagccggcct tggtagacac catcgtctgg
     3001 cgccgcggcg agaacgcgta agaaagaaac ctcgccacga ggacacgaag tgcacgaagg
     3061 gaagaactca taaataaaaa ttcttcgtga ctttcgtgct cttcgtggtg caagagactt
     3121 aaaccgatcc aaggaggaaa attccatggc aaagcctgcg ccgaaagcag agatcataaa
     3181 aaaatcagac aagcgctggc agtccgacgt tatcgtcgac atgatcaagc gctacgattt
     3241 cgaatacatc gcactcaatc ccggcgccag ctaccgcggg ttgcacgact cattggtcaa
     3301 ctacggcgag aacgacccgc cgatgctgct ttgccagcac gaaaaaatcg ccgtgcaaat
     3361 cgcccacggc tacgcccgcg tcaagggcaa gccgatgatc gccatcgctc acaacttagt
     3421 cgggttgctg cacacaccca tgggtgtcta ctatgcctac ctcgatcgcg ctccggtgtt
     3481 cctcataggc gccaccggcc cgttggccga gccgaaacgc cgcccattca tcgattggat
     3541 ccacaccgcc aatgttcaag gtgaagcgat tcgtaatttc gtcaagtggg attatcaacc
     3601 gagcaccgtc gatggtgtgc cgggcgcgtt cgcgcgagct tactcggtca tgatgagcgc
     3661 gcgacaaggt ccggtttata tgtgctacga tgccggtctc caggaggcgg aactggatca
     3721 tgacgtggag atgccgccgc ccggggcaat cgacgttccg accgcgccct cggccgatcc
     3781 ggccgcgctc gaaaaagccg ccgacacttt agccgccgcc aatcgagtgg cgattatcgc
     3841 cgattttgct gcccgcccgc cgcacggatg gaatcatatt gtcgagctgg cagaaacatt
     3901 aggggcttcg gtatgggatg tgggttccag gcttaatttc ccgaccaatc atccgctgaa
     3961 tctgaccatg gatcctgaag gttgctataa agatgtcgac gtcgtgctaa ccctcgatat
     4021 cgccgacttc gaaaagccga ctcatatacg tgacatctcg actcgcacgg tcgtcagcaa
     4081 ggtccaggag aaggcgacct ggatcgacat cggctttact gacatcgaaa tcagcaagtg
     4141 gtcgatggat tatgcgcggc cgttctatgc gcaacagcgc atgatcgccg acccggttac
     4201 cacggtgccg cttctgacca agcttctcaa ggcgcgcatc ggcaatacca aagggcttag
     4261 tgaaaagatc gccaggcgaa atgaagaaat cggcaaacgt catgctgaga atcgcgccaa
     4321 gtggagcaag caggcgcaag agcactggga tgccaagccg atgactgtgc cgcgcttggc
     4381 gctcgaagtc tgggaagcga tcaaaggcga cgattgggtt ttgactaccg gggatttggg
     4441 cagctgggga aagaagcttt gggatttcga ccggccgtat tgccatggag gccgtgagct
     4501 cggcaccggc acgcaaatcg gcctgtcact cggggtggcg ctggcgaaca agggtacagg
     4561 tcggctggtg gtcgatttgc agcctgacgg cgatctgatg ttcgatgccg gagcgctgtg
     4621 gatcgcggca aaatacaaaa ttccgatgct catcgtcatg ttcaataatc gcgcctacta
     4681 caacgactgg aaccatcaaa ttgtcatggc gaaaaaccgc ggcaccgatc catcgcgggc
     4741 gcatatcggc atggaccttt atgatccgga tcctgatttt gctggtctcg cccgttcgat
     4801 gggttggtat gccgaaggac cgttcgaaac cggcgatgat ctaaagcctg cgcttaaaag
     4861 ggccatcgag caagtcaagc aaggtaaacc cgcgctgatt gatgcggtat gcgaccggcg
     4921 caatcacggc taggctttat actccaatgt ggtgcgggcg gcttatttag atcaatgcga
     4981 gcagcagtgc atcgacttaa cgttttacat tagaaaaacg ttgacaaatg catcgttgat
     5041 cagataacta tgtgtgcgag ataaagccgc ttgcctgctg atcgctcatc ctagcaatag
     5101 gcaaacggca ttatgtcgtc ggtacggggg taacacatga aagctttctc ggcgggatgc
     5161 gcattgatga tgctcgtggc gctactagcg tctcctgcaa tcggggctga gcgctcgaca
     5221 gcgtacaact ggacgggatt ttatgctggc gtcaatggcg gcggcggctg gggtcagtcg
     5281 cgtcatgatt tcgaggcggc cggcacgaca acgggagact atcgtatctc aggcgcaacc
     5341 gcaggcggca cactcggggc caatctacag gccggcggac ttcttttggg tgttgaagga
     5401 gatatgaatt ggtccgacat tggcggcagt gattcctgcc cgaacgcgaa ttttacctgt
     5461 cggacgcgaa gtaagtggct tgccaccact cgtgggcgcg taggttatgc gatcgaccgt
     5521 gttctcccat acatcacggc cggcgccgcc tttggcagga ttcgcgcaca aattcctgat
     5581 tttggcagtg aaagggaaac cgaagtgggt tggacggcag gcggcggact ggaagctgga
     5641 atattcggtg ggcttagcgc caagctggaa tacctctacg ttgatttcgg cacgtttaat
     5701 tgcggcttcg cttgcaccga tactctggtc gacaaagtta aattcaacgc gcatatgttt
     5761 cgtgtcgggc tgaattatcg gttctgagcc cgcgatgagt gggttgtcga ttacgggttg
     5821 acttaccccg gcagtgcggg cccgtgcata ggggagaacc cggctttaag acttgttgtt
     5881 tgcgcgggat accgatcagt gtactggctg ggagagatcc tcaattactt aacat
//
LOCUS       DASUTG010000505         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2004134, whole genome shotgun sequence.
ACCESSION   DASUTG010000505 DASUTG010000000
VERSION     DASUTG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2004134"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VF089_11605"
     CDS             complement(<1..1425)
                     /locus_tag="VF089_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX6174651.1"
                     /translation="MDEPAPPASETSVPRLEKLRTVLNTCKKMSSVGDIISLLDHITT
                     QAAELLEADRASIFLLDREKCELWSIITHEEKKRMVMFDARLGIAGAAAMSGQTINVA
                     DAYEHPLFFKEVDQKIGYRTKTLLAVPIMNAKGEIIGVGEAINKKVGMFTDEDAEILR
                     SLAAHICDATKTTHIDTVLTNRQQREGNDEIGGEFSTRNIIGMSYRIQSVVRLIDQIR
                     DSSVDVMIQGESGTGKELVAKALHYNSPRSKQPFVAVNCAALPDSIVEAELFGIERGI
                     ATGVDRRIGKFEEAQGGTLFLDEIGDLSLPSQAKILRVLQERSVTRVGARTATPLDLR
                     IIAATNKDLEREIKDGHFRVDLYYRLKVIHIETPALREVPEDIPLLATHFLEKYSKLM
                     KTEPKQFTPAALECMASYGWPGNERQLENEVRRLVASVRGKIIAEEHIDPSIRSSEKT
                     LASSTIPSALKTPQTETAPETSRPL"
     gene            complement(1559..2572)
                     /locus_tag="VF089_11610"
     CDS             complement(1559..2572)
                     /locus_tag="VF089_11610"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX6174652.1"
                     /translation="MRMIVIGTGPIGGIIGGRLARAGHDITFVDVDQEHIAAIRANGL
                     QVDVPDGAFNVRVNIVLPEEIQGKFDIGFIAVRSNYTPDALATVLPALNHDGLLVSMQ
                     NGINPPLLEEKVGTDRTVGMAIRMGCRRVAPGHVQTSTRGHLYIGHSHGRTTPQLNRL
                     HELLNAVIDTEISDNILGVLWSKLTYTCLGFFGSLADVALATSCAEDSGRRLLADFFA
                     EVIAVGTAAGARFIRLAEYNPPDLHPSIPIDKRLAVINEMAKSWKEDDRKGPLRQIQR
                     GIPTEVDYTLAYVVREGEKQNIETPLCRKLLTMMHELEAGTRQLDMKNYAELQAVQTD
                     RHL"
BASE COUNT          568 a          749 c          725 g          702 t
ORIGIN      
        1 aagaggcctg gatgtctccg gagcggtttc cgtctgcggt gttttcaatg cgcttgggat
       61 cgttgagctt gcaagcgtct tttctgaact gcgaatcgac ggatcaatat gttcctcggc
      121 aataattttt cctcgaacag aagcgacgag gcgcctcact tcgttttcta gctgccgctc
      181 gttccccggc cacccatagc tggccatgca ctccaaagcc gccggtgtaa actgcttcgg
      241 ctcggtcttc atcaatttgc tgtatttctc gaggaaatga gttgccagga gcggaatatc
      301 ttccggtact tcccgcaaag ccggcgtttc gatgtggatc actttcaaac ggtagtaaag
      361 atcaacgcga aaatgcccgt ccttgatttc acgttccaga tctttgttgg tggcggcgat
      421 aatccgcagg tccaggggcg ttgccgttcg ggcaccgacc cgggtgacag acctttcctg
      481 gagaacgcgc agaatcttgg cttgcgaggg caggctgagg tcgccgattt cgtccaaaaa
      541 tagcgtgccg ccttgggcct cttcgaattt gccgatgcgc cggtctacgc cggtggcaat
      601 gcctctttcg ataccgaaca attccgcttc gactatgctg tcaggcagag ctgcacagtt
      661 aaccgctaca aacggctgtt ttgaccgcgg actattgtag tgcaaggcct tagccaccaa
      721 ctccttgccg gtgccactct cgccctggat catcacgtcg acggagctgt cgcgaatttg
      781 atcgatcaag cggactactg attggatccg ataactcatt ccgatgatgt ttcgggtcga
      841 aaactcgccg ccgatttcat cgttgccttc tctttgttgt cggtttgtta gtaccgtgtc
      901 aatgtgagtg gttttagtgg catcgcaaat gtgcgccgcc agagatcgaa gaatctccgc
      961 atcctcgtcc gtgaacattc caaccttttt gttgatcgct tcgccaactc caatgatttc
     1021 acctttggca ttcatgatcg gcactgccag caacgttttg gtacgatagc caatcttttg
     1081 atcgacctcc ttaaagaaca atggatgttc atacgcatcg gcgacgttga tcgtctgacc
     1141 gctcatcgcg gctgctccgg ctatccccaa gcgcgcgtcg aacatcacca ttcttttttt
     1201 ttcttcgtgg gtgatgatcg accagagctc gcatttctcc cgatcgagta gaaaaatgct
     1261 cgcccgatct gcttcgagga gttctgcggc ttgtgtcgtt atatgatcca gaagtgagat
     1321 gatgtcgcct acagaggaca tctttttgca ggtattgaga actgtccgca acttctccag
     1381 gcggggcacc gaggtttcag aagcgggtgg ggcaggttcg tccatccgcc tctgagtaac
     1441 acatccgcaa aaacgctgtc aatgtgaggt cacgcggcgc cgatggggtc agtgcgcggt
     1501 tgccgcgctg ctaaggatga acgttaagtc gaggtctctg tgacccgtag gaatatcgtc
     1561 agagatgtcg atcagtttgc accgcttgga gttctgcgta atttttcata tcgagttgcc
     1621 tagtccctgc ttccaactcg tgcatcatcg tcaataattt gcgacagagc ggcgtctcaa
     1681 tattctgctt ctcgccttcg cgaacgacgt acgccaaggt gtaatcgact tcggtgggta
     1741 ttcctctttg aatctgccgc agcgggcctt tgcgatcatc ctctttccac gatttcgcca
     1801 tttcgttgat aacggcgagt cttttgtcaa tgggaatgct tgggtgaaga tccggggggt
     1861 tgtattctgc gagtctaata aagcgtgccc ccgccgcagt tcctactgcg atgacttcgg
     1921 caaaaaaatc cgccagcaac cgccggcccg aatcttcggc gcaactggtc gccagcgcca
     1981 catctgcaag agacccaaag aagccaaggc atgtataggt gagcttgctc caaagcacgc
     2041 cgaggatgtt gtcggaaatc tcagtatcga tcactgcgtt cagcaactcg tgcagccggt
     2101 tcagctgcgg cgtggtcctg ccgtgcgaat gaccgatgta aaggtgcccg cgggtggagg
     2161 tttggacatg accgggagca accctgcggc aacccatgcg gatggccata cccaccgtgc
     2221 ggtcggtgcc gaccttttct tcgagcaggg gaggattgat gccgttttgc atcgagacca
     2281 gcagtccatc atggttcagc gccggcagga ccgttgcaag cgcatcagga gtatagttcg
     2341 aacgcacggc gataaaaccg atgtcgaact tgccctggat ctcttccgga agcacaatat
     2401 taactctgac gttaaaggcg ccgtcaggca cgtctacctg caagccattg gcgcgaattg
     2461 ctgcgatatg ttcttgatca acatcgacga aagtgatgtc gtggccggcg cgcgctaacc
     2521 ggccgccgat aattccgccg atgggtccag ttccgattac gatcatgcgc atggagaaga
     2581 tcctttccta gtatttataa cgagtccgat ctactcttat gcgaaatcaa gcgaggcggt
     2641 caaatcgttc cgtcgggccc catccaacgg agccgcgttc ccagctacct gtggttcttg
     2701 tcttgaccct gctttcacaa gatctctata cttagttatg attc
//
LOCUS       DASUTG010000506         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1262413, whole genome shotgun sequence.
ACCESSION   DASUTG010000506 DASUTG010000000
VERSION     DASUTG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262413"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..543
                     /locus_tag="VF089_11615"
     CDS             169..543
                     /locus_tag="VF089_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174653.1"
                     /translation="MTPLLLIVDDEFGVRESLKVVFCKDFRLVEADSVDTAIPLVIGS
                     RPDVVLLDVLMPKTDGIEALRRIKELQPACEVIMLTGVNSQQLSSKALDFGAFDFVAK
                     PFDVVDLRQKVVRALQKIQQQK"
     gene            complement(593..1252)
                     /locus_tag="VF089_11620"
     CDS             complement(593..1252)
                     /locus_tag="VF089_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174654.1"
                     /translation="MIKQCAVLFGLFFMAPLVSVLGAQEQPTVAEYRDPEYAYSFQYP
                     AHWKLKKLPEGAANDEVRVSLQTPSGDSFMVIIEKRGQNPSKAEFQSDPKRSARVDEL
                     IDETTQEIYRTVARNLGAVETKFGEKTDLSNDAAIKFYVSALNKMKAGEPVIVAGIHS
                     YPFSKPYSISFIMTAFFNPDAEKENALMRAVFNSFQLLEPPESSSGAGSTGSSKGNQP
                     K"
     gene            1434..3434
                     /locus_tag="VF089_11625"
     CDS             1434..3434
                     /locus_tag="VF089_11625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6174655.1"
                     /translation="MTTQYILKNSTEPIRNSSGLDYRKELNEAQYRAASTVEGPLLII
                     AGAGTGKTRTLVYRVAHLIDSGVDPRSILLLTFTRRAAEEMIRRAGLLIDSRCHQVAG
                     GTFHSFANLVLREYGRRIDLLPSFTIMDRTDSEAVIQEIRAEMGLNTKDRRFPRKQTC
                     AEIFSMALNKQTPLGELIELEYPHLIEANEELIDLNRRYINYKQTKSLVDYDDLLTKL
                     KDLLASHEPVRNRLSDRFRFIMVDEYQDTNHLQSKIVRLLAATHDNVAVVGDDAQSIY
                     SFRGADFRNIMDFPKHFPGARIIKLEENYRSTQPILNLTNEIIQRAAEGYDKRLFTRK
                     SVGPRPRLVQAGSEQTQSQFVCQKILELREQGVPLWDMAVLFRSSFHSFDLEIELARQ
                     NIPFVKRGGFQFMEASHVKDLLAHLRILANPRDAISWSRVLLLLEGIGPGANQKIINW
                     LLDGGNPIERLRTYPARGKVAQGLKTLAQVLEVTSEAELPAEQAQYLMQYYTPILKQN
                     YPDDHPKRLRDMEHFQGMTERYRNLERLLSDMALEPPNDSIAGVLAVNPDEGPLILST
                     IHSAKGLEWNSVFIIWALEGRFPSIYNTNTTEELEEERRLLYVAATRAKQNLFISYPT
                     RVFDKSLRMVLSRPSQFVDGISHRLLEPVSLVPEGSLWSWES"
     gene            3439..>4195
                     /locus_tag="VF089_11630"
     CDS             3439..>4195
                     /locus_tag="VF089_11630"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX6174656.1"
                     /translation="MVQLLLGFVVFVSGAVLMSLEIAGSRILAPHFGSSIFVWGSLIS
                     VVMASLSIGYYWGGWLSARDPSYGRLLILLLIPGIVIFFLPFIYPAANEWVARNDFGM
                     RLNPLIACTILFLLPGVFLGTISPYAIRLAATTLSTVGSTAGTLYAVSTCGSIFGTLL
                     TAFYLIPAAGVRNIIHGLGITLVCLSLFVVPLVRLRGITLARAVTAVTVILGSFMMPW
                     TPGALARIKTLVEKETFYHRIRVEEDDEARYMYF"
BASE COUNT          949 a         1143 c         1111 g          992 t
ORIGIN      
        1 gaaagcatcg gctatctaaa aacgactttc caaaaatcaa ttgaggagat taccagggac
       61 tgcgcacaat atctgggcca gccgaaacgc tgcgtggatc tggcatcgcg ttaaccagcc
      121 cgctaagctg tgtgacgaac aggagttatg gttgtggttc tctaaccgat gacaccgctt
      181 ttactgattg ttgacgatga atttggagtc cgtgaatcac tcaaggtggt tttctgcaaa
      241 gactttcgcc tggtggaagc ggattcggta gacacggcaa ttccgctagt aataggttcc
      301 agaccggatg ttgtcctgct cgacgtgttg atgccgaaaa ccgatggcat tgaagctcta
      361 cgacgcatca aggaactcca gcctgcctgt gaagtcatca tgctcactgg ggtgaactca
      421 cagcaactat ccagcaaagc tttggacttc ggagccttcg atttcgtggc taagccattc
      481 gatgttgtcg atctgcgcca aaaagtcgta cgagccttgc agaaaatcca acagcaaaag
      541 taagagctga ttgatggacc aaatgagaag agatatcgtt cgatgccgcg gcttatttcg
      601 gctgattgcc ttttgaagaa ccggtggaac cggcgccgct cgaagactct ggcggttcca
      661 gcaattggaa cgaattgaag actgccctca tcagggcgtt ttctttttca gcgtccggat
      721 tgaaaaaggc ggtcatgatg aagctgatcg aatacggctt ggaaaagggg tacgaatgaa
      781 tacccgcgac aattaccggt tcccctgcct tcatcttgtt cagggccgag acgtagaact
      841 tgatagcggc atcgtttgag agatcggtct tctcgccgaa ttttgtctcc accgcaccga
      901 gattacgagc aacggtccgg tagatttctt gcgtagtctc atcgatcagc tcatcgactc
      961 gggcgctgcg cttggggtcg gactgaaatt ccgccttgct cggattttga ccgcgcttct
     1021 caatgatcac cataaaggaa tcgccgctcg gtgtttgcag cgagactcga acttcgtcgt
     1081 tagccgctcc ttccggcaat ttttttagct tccagtgggc tggatactga aaactatagg
     1141 catactcggg atcgcggtac tcggcgaccg tgggttgttc ctgggcgcct aggacgctca
     1201 cgagcggcgc cataaaaaac aacccgaaca agactgcgca ttgcttgatc aaaatttcgc
     1261 tccccatccc tggttttaag gactgagact agcacacgag accgatgatg aaagcccagg
     1321 caagaaacag gctgttgctg tgacgcgatt aaccgcgggc gaagcaaaac attgtccgtc
     1381 atcgcttgct gatattattt aaaataggta atttacgtcc gattggagtt tgcttgacca
     1441 cacaatacat tctcaaaaac tcaactgaac cgatccgcaa tagctcaggg ctcgactacc
     1501 gcaaagagtt aaacgaggcg caatatcggg cggcaagcac ggtcgagggg ccgctgctga
     1561 tcatcgccgg cgccggcacg ggaaagactc gcaccttggt ctatcgggtc gctcacttga
     1621 tcgacagcgg agtcgatcct cgttcgattt tactgctgac ctttacccgc cgcgccgcgg
     1681 aagaaatgat tcgtcgcgcc gggctgctaa tcgacagccg ttgccatcag gtggccggcg
     1741 gaaccttcca ttcgtttgcc aatctcgtgc tgcgtgaata cggccggcgc atcgatctat
     1801 tgccttcttt tacgatcatg gatcggaccg acagcgaagc tgtgatccaa gagatccgag
     1861 cggaaatggg tttaaacacc aaagaccgcc gctttccacg caagcagacc tgcgccgaaa
     1921 tcttcagcat ggcgcttaac aaacagacac cgttgggtga gctgatcgag ctcgaatacc
     1981 ctcatctcat cgaagccaac gaagaactga tcgatttgaa tcgacgttac atcaattaca
     2041 agcaaaccaa gtcgctggtg gactatgacg atctcctgac taaactcaaa gacctgttgg
     2101 ccagccatga accggtgcgc aaccgcctgt cggaccggtt ccgcttcatc atggtcgatg
     2161 aatatcaaga caccaatcac ctgcaatcaa agatcgtccg gctgctggcg gcaacccacg
     2221 acaacgttgc cgtagtcggc gacgacgctc agagcatcta ttcctttcgc ggcgccgact
     2281 ttcgcaacat catggatttc ccgaagcact tcccgggcgc gcggatcatc aagcttgagg
     2341 aaaactaccg cagcacccag ccgattttga atcttaccaa cgaaatcatt cagcgcgccg
     2401 ccgaaggcta tgacaagcgt ttgttcacgc gcaagagcgt cggtccacgg ccgcggctgg
     2461 tgcaagccgg cagcgaacaa acacaatcgc agtttgtttg ccagaagatc ttggagctgc
     2521 gcgaacaagg ggtgccgctg tgggacatgg ctgtgctatt tcgctccagc ttccattcct
     2581 tcgatctcga aatcgaactc gcgcggcaaa atattccatt cgttaaacgc ggcggtttcc
     2641 agtttatgga agcttcccac gtcaaggatc tgctcgcgca tttgcggatt ttggctaatc
     2701 cgcgggacgc gatttcctgg agccgggtgc tgctgctact cgagggcatt ggaccgggcg
     2761 caaatcagaa aatcatcaac tggcttctag acggcggtaa tccaatcgag cggttgcgca
     2821 cctatccggc cagaggcaag gttgctcagg gtttgaaaac attggcgcaa gtactggaag
     2881 taacttccga ggccgagctg cccgcggagc aggcgcaata tctgatgcaa tattacaccc
     2941 cgatactcaa gcagaactat cccgacgatc atccgaaacg tctacgggac atggaacact
     3001 ttcaaggcat gaccgaacgt tatcgcaatc tcgaacgtct gctgagcgac atggcgctgg
     3061 aacctccgaa tgacagcatc gccggggttc tcgcggttaa tccagacgaa ggtccgctca
     3121 tcctgtcgac gatccattcc gctaaagggc tcgagtggaa ctcggtgttt atcatctggg
     3181 cgttagaagg aaggttcccg tccatctaca acaccaacac gaccgaggag ttggaagaag
     3241 aaagacgttt gctgtacgtc gcggcaaccc gggccaagca gaaccttttt atctcttatc
     3301 caaccagagt tttcgacaag agcttgcgca tggtgctgtc ccgcccctcg cagttcgtgg
     3361 acggcatctc gcatcggctg ctcgaaccgg tctcactggt gcccgaggga agtctctgga
     3421 gttgggagag ttagattgat ggtgcaattg ttgctcggct tcgtagtgtt cgttagcggt
     3481 gcggtcttga tgtcgctgga gatcgccggc agccgcattc tcgctcctca tttcggcagc
     3541 tcgatctttg tctggggcag cctgatatcg gtggtgatgg cttccctgag catcggctac
     3601 tactggggcg gctggctttc ggcccgggat ccctcttatg gtcggctgct gatcctgctt
     3661 ctgattccgg gtattgtgat cttctttctt ccgttcattt atcccgcggc aaacgaatgg
     3721 gtggcgcgca atgatttcgg catgcgctta aacccgctga ttgcgtgtac catcctcttt
     3781 ttattgcccg gcgtgtttct cggaaccatt tcgccctatg cgattcggct ggcagcgacg
     3841 acactttcca ccgtcggcag caccgccggc actctctacg cagtgtcgac ctgcggcagt
     3901 attttcggca ccctgttgac cgccttttat ctgatccctg cggctggtgt ccgcaatatc
     3961 attcacggac tcgggattac cctggtttgt ctgtcgctat ttgtagtacc tttggtccgc
     4021 ctgcggggca ttacccttgc ccgggcggtt accgcggtca ctgttatact cggatccttc
     4081 atgatgcctt ggacacccgg cgctctggca cggataaaaa cactcgtcga gaaggaaact
     4141 ttctatcacc gcattcgcgt cgaagaagac gatgaagcgc gatatatgta cttcg
//
LOCUS       DASUTG010000507         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_818338, whole genome shotgun sequence.
ACCESSION   DASUTG010000507 DASUTG010000000
VERSION     DASUTG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818338"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1296)
                     /locus_tag="VF089_11635"
     CDS             complement(235..1296)
                     /locus_tag="VF089_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174657.1"
                     /translation="MADLHAVPETPEVQALHRRLAEYSLGGHWQPREKNPDLVPHLWP
                     WSAIYSCLMESGEVIKLGGIDDAAKRRTVQLVNPALTNKKATTRSIQMSIQLVKPGER
                     AECHRHTADALRFIVEGDGTGYTNVEGEQMLMEPGDLVLTPNWTWHDHYNAGSRNLVW
                     LDVLSVPLVSHLDANFHENYGEGPAQPIVKPDGYGRQKYGAIRPRMTNVSNAALPYNY
                     KWRDTLAALNEIAAASERDPHDGILLEYAHPLTGGPTMATIGCWVQMVPPGKSTTPHR
                     HTSSTIYHVVEGEGLATVGMRKGAGKELGWGPHDCFFVPSWNWHHFENKSKKAPAIIF
                     SVTDRPVLESLGLFREEAA"
     gene            1742..2680
                     /locus_tag="VF089_11640"
     CDS             1742..2680
                     /locus_tag="VF089_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174658.1"
                     /translation="MWFLLLVISIVVVTPAAHLYAQTQKIRIAHSSRSNTVTPFYVAA
                     TRGFFREEELDVELIQVNPRLGAMAVMNGDVAFTTSFTSTFRGILQGLPLKVVLIALK
                     KGVYFLVARPEVRDIQDLKGKKIGVSTVRGTDQLVAEEMLRAKGFDPSLLQVVALGDT
                     AVRAQALAAGVVQAVTLSPPHDLIVQQMGHKVLAGPPEIGLPASGLFTSDRLLKENPQ
                     AVKRTLRALLKANRFIAENQQETIRIMLKWVPQSPEIAARSYDLELKGITQDGQMTDA
                     ELESLIERLGDKKRPLDEVRDFSLVRQALKEFTLGK"
BASE COUNT          599 a          761 c          802 g          650 t
ORIGIN      
        1 atgaacgcct ggtagtcact ttgttcgagg aaaatctttt gcccgtcatt gcaacgggcc
       61 atcacgtgat aaagcagtcc ttcggcatgg actcgcggat gacgtcacat cttctttcac
      121 tatcaggcat ttgtaagttt aggcacgtct gaccgggctt tcttgtcgcg aggttcttca
      181 tgccacatgg gctccgacgc aatggtagac ggctaagcta agtcgcctca taccttacgc
      241 ggcttcttct cggaatagac ccaaactttc caacaccggg cggtcggtta ctgagaaaat
      301 gatggccggt gcctttttgg acttattttc gaagtgatgc cagttccacg acggcacgaa
      361 gaagcagtcg tgggggcccc agcctagctc tttcccggcg ccttttctca tccccacagt
      421 agcaagcccc tcgccctcga cgacgtggta tatcgtgctg ctggtgtggc ggtgcggagt
      481 cgtgctttta cctggcggca ccatctgcac ccagcagccg atggtcgcca tggtcggtcc
      541 gccggtaagc gggtgagcgt actcgagcaa aatgccatcg tgcggatcgc gctcgcttgc
      601 ggcggcgatt tcattcaacg ctgccagcgt gtcgcgccac ttatagttgt aaggcagcgc
      661 ggcattgctg acattcgtca tccgcgggcg aatggcgccg tatttttgcc gaccgtagcc
      721 gtcgggcttg acgatcggct gggctggccc ttcgccgtag ttctcgtgga agttggcgtc
      781 caaatggctc accagaggga cgctgaggac gtcgagccag accaagtttc tactgccggc
      841 gttataatgg tcgtgccagg tccagttggg tgtcagcacc aggtcgccgg gctccatcag
      901 catttgctcg ccttcgacgt tggtataacc ggtgccgtct ccctcgacga taaagcgtag
      961 agcgtccgcc gtgtgacgat ggcactcggc gcgctcgccg ggtttgacca actgaataga
     1021 catttgaatc gaccgcgtgg tggccttttt gttcgtcaac gcgggattga cgagctgcac
     1081 ggttcgtcgt ttggcggcat cgtcgatgcc gcctagcttg atcacttcgc cggactccat
     1141 caggcaggaa tagatcgccg accagggcca gagatgaggc accagatcag ggttcttttc
     1201 gcgcggctgc cagtggccgc ccagcgagta ttccgcgaga cgccgatgaa gagcttgtac
     1261 ttcaggggtt tccgggacgg cgtgtagatc ggccatggcg actctcctca taatgttcgt
     1321 ttcctagacg ccactttatc gccgggccgc agagactgtc aacatatgtc cggtcttttt
     1381 gccattcgcc gataccttga gcccaacaaa ccattcttgt ttggactaaa ccgctgatga
     1441 aatcctcgcc aacgtgccac gatgttgtca gcgcatctct gactcacgac gctggatgtg
     1501 gcgttgacgt ttgtttcagt acacatgcac gatccgccgt tgacagctct gacctatttg
     1561 ctgtactggc ttaaaagcac atggggagcg aaacaatgag aagagctttc gggggtcgtg
     1621 gcaatgcacg ccaagctgct tcgtcaaagc gccttgagcg acgcaactga ttctcccatg
     1681 gtcaatgata tccggtcgca atagcgagat tgtcatgcga gcattgctca tacgatggtt
     1741 catgtggttt ttattactgg taatttcgat tgttgttgtg accccagcag cgcatctgta
     1801 cgctcaaaca cagaaaatca gaatcgctca ttcgagtcgc agcaacacgg tcacgccctt
     1861 ctatgttgcg gcaacacggg gcttcttccg tgaagaagaa ctggatgtag aactcatcca
     1921 agtcaatccg cggctcggcg cgatggcggt gatgaacgga gacgttgcgt ttacgacctc
     1981 ctttacgagt acgtttcgcg gcatcctcca gggactaccg ttgaaagtcg tcctgattgc
     2041 tctcaaaaaa ggcgtctatt ttttggttgc ccgtcccgaa gtaagagata tccaggatct
     2101 caaggggaaa aagatcggcg tctcgacagt caggggcacc gatcaactcg tcgccgaaga
     2161 gatgctccgg gccaaaggat tcgatccctc attgctgcaa gtagttgctt tgggggacac
     2221 cgccgtaagg gcgcaggctt tggcagccgg agtggttcag gcagttacac tgtcgccgcc
     2281 gcacgacctg atcgtgcagc agatgggaca taaggtattg gccggcccgc cggaaatcgg
     2341 tctgccggca tccggattat tcacctccga tcgtcttctc aaagagaacc cgcaggcggt
     2401 caagcggacc ctacgggcgc tgctgaaagc taaccggttt atcgctgaaa accagcagga
     2461 gacgatccgc atcatgctta aatgggtacc gcaatcgccg gagatcgccg cccgctctta
     2521 tgatctcgag ctgaagggca tcactcagga cggccaaatg acggatgccg aattggagag
     2581 tctcatcgaa agattgggtg ataaaaaaag gcccttggac gaggttagag atttttcgct
     2641 tgtgcgccag gctctgaaag aatttacgct gggcaaatga tccctctcga ggaaaagcgc
     2701 gggaaaacgc cttggaaaaa gccaaggaga ccaagggagg cgcacgggga atttgagagc
     2761 gagtgtcctg gttactgtgg agcgcaggat accttctatg taggaacact aa
//
LOCUS       DASUTG010000508         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_818497, whole genome shotgun sequence.
ACCESSION   DASUTG010000508 DASUTG010000000
VERSION     DASUTG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818497"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF089_11645"
     CDS             complement(<1..563)
                     /locus_tag="VF089_11645"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174659.1"
                     /translation="MMLPALLIILIVAVIPSELPAQERYSVSYGGFAGYHAPLWAAKD
                     LGLFAKHGLNADPVMIAGSARGMQALLSGSTHFALADATGPVSALYQGADVVLVASAM
                     NKFPFSMYAQKEITRHADLKGKRIGIASFGGATEWGLNLGLREWNIPRDSVTVTAHGP
                     GANRLLALSTKGVDAALLAPPDRPRQSV"
     gene            1016..1990
                     /locus_tag="VF089_11650"
     CDS             1016..1990
                     /locus_tag="VF089_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174660.1"
                     /translation="MKTLLIALGFFIASFCNSSAHAQLKMTVGQAGINPGTSLYFIAQ
                     KENLFAKHGVEVNIVATTTTAAVQGMLGGSLHLTLGAGPAFIAATLEGSPPFAVISSW
                     VNVFPYYIVARKEIAKIADLKGKTGQVGATFGSAPDVALRFGLSKLGLNPEKDVKLVQ
                     MPRPDWINVIGQLEQGDVQFSVLPPPYDRMGAPKGIHALYALPDLGIHWQQNGEWVLK
                     SYAGKNREALLRFERALADAMTVYFNQKEKTIGYLMEFLGSNRDDTEYAYQAYAKWAD
                     RNPKPTTESIKNTLTAIAATTPAAANANPTAFIDTSFVDQLLKEGYFK"
     gene            2247..2870
                     /locus_tag="VF089_11655"
     CDS             2247..2870
                     /locus_tag="VF089_11655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX6174661.1"
                     /translation="MEISKKIEFHPDSTALIIVDMQVEGCERHGPGMKPVIANIRRLL
                     DRFRAINGKIIHVQSVRTKDHPEFTVFGRPYGLLLGSPGADFVEELKPLPGEDVVQKT
                     SHDCFYKTSMEPTLEKLDLQPCRDKIVVTGIGSSNCVYHAVIGFHIRNYYSIVPEDCI
                     HGVHPQSQPFALHQFRSNAYNFNVTVSRSEDLRLISDGVGLERAVSR"
     gene            2878..>3686
                     /locus_tag="VF089_11660"
     CDS             2878..>3686
                     /locus_tag="VF089_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174662.1"
                     /translation="MMDTLRSKLKIVFIVFGLFNIPVSAMAQRLTVPFAALSPTYAPL
                     WVAQEAGFFKKYGLDVQVIYISAGSVIIPAILSGQVDIANMSSAPALTAWSRGAELAT
                     VGVTSNRLLHVVMARGSIKKPEDLKGKKIGSDRYGSLSDLVLREALRHYNIAPDRDVA
                     VIQTGGLPERLGALKIGAVDGAIVTGDTAFEAEKLGFHKVIDLSQLPIRYPGSTIVVT
                     RSFLSGKREIVKRFLRGWIEGIKTAKTDKEFTIKVMQKFLKTGDRSILDKT"
BASE COUNT          840 a          999 c         1021 g          826 t
ORIGIN      
        1 acgctctgcc tgggccgatc cggaggcgct aacagtgccg catcgacgcc tttagtcgac
       61 agcgccagaa gccggttggc gccgggaccg tgagcggtga ccgtgactga atcgcgcggg
      121 atgttccatt cccttagacc aaggttaagc ccccattccg tggcgccgcc gaaactggcg
      181 attccgattc gtttgccctt gaggtcggcg tgccgggtga tctctttctg ggcgtacatg
      241 ctgaagggga atttgttcat cgcgctggct acgagaacga catcggcgcc ttgatagagc
      301 gcgcttaccg gaccagtggc atcggcaagg gcaaagtggg tgctgcccga aagcagcgcc
      361 tgcatgcccc gcgccgaacc ggcgatcatc actggatcgg cgttgaggcc gtgcttggcg
      421 aagagaccca ggtccttggc agcccataaa ggcgcgtgat agccggcgaa gccgccatag
      481 ctgaccgaat agcgctcctg cgcgggtagc tcggacggaa taaccgcaac gataaggatt
      541 attaataaag ccgggagcat cattgttttc tccttcccgc ggcttaatcg cgggcattgg
      601 ataatcgcgt ggtgttgctt gtcaagcgcc ggatgttttc cgtcttcccg cgagcgcgtc
      661 cttggcggtg acgtcttgtt tggatctacg aaaactttgc cattgtatat gccgagcgct
      721 gttgggttag acgatcgata gtaaacaggc tgtaaagagg cggcgcggta gcaaatgcat
      781 tggctgcgac ccagttcaat gcgttgacct gcaggctcta agctggggcg ccaagatgca
      841 ttcgatcgcg tctgttgaag aaataagagc actattccgc cttagcagcg gatctgtggc
      901 gatcattaaa agactcctgt aaattcgccc cagggcaagc tgttggcggt ctccgaagcc
      961 attgacatcg atctcgcctc ggtggaaaga tcgccccacg agaggagcta tccaaatgaa
     1021 gaccctgctc atcgcccttg ggttttttat agcttcgttt tgcaacagct ctgcccacgc
     1081 gcagctcaaa atgaccgtcg gccaagcggg catcaatccc ggcaccagcc tctatttcat
     1141 cgctcagaaa gagaatctct tcgccaagca tggggtggaa gtaaacattg tcgccaccac
     1201 gaccaccgcc gcggttcaag ggatgctcgg cggcagcctg catttgaccc tcggcgccgg
     1261 cccggcgttt attgccgcaa cattggaagg gtcgccgccg tttgcagtga tttcttcgtg
     1321 ggtgaatgtt tttccctatt acatcgtggc gcgcaaggaa atcgccaaga tcgccgatct
     1381 caagggcaag accggtcaag tgggcgccac cttcggatcg gcgcccgacg tggccctgcg
     1441 ctttggcctg agcaagctgg gtttgaatcc ggaaaaagac gttaagctcg ttcagatgcc
     1501 gagacccgat tggatcaacg tcatcggcca actcgagcaa ggcgacgtgc agttttccgt
     1561 gctgccgcct ccctacgacc gcatgggcgc gccaaaaggc atccacgcgc tctacgcgct
     1621 gccggacctc ggcattcact ggcaacaaaa cggcgagtgg gttttgaaat cctacgcggg
     1681 gaaaaaccgg gaagccctgc tgcgttttga acgcgctctg gccgatgcca tgactgtgta
     1741 tttcaatcag aaagaaaaaa ccatcggcta cctcatggaa tttctcggga gcaatcgcga
     1801 tgacaccgag tatgcctacc aggcttacgc caaatgggcc gataggaatc ccaagccgac
     1861 aacggaatcg atcaagaata cgctaacagc cattgccgcc acgacgccgg ctgccgccaa
     1921 cgccaatccg acggcattta tcgacaccag cttcgtcgat cagttgctga aagaaggata
     1981 tttcaaataa taatcctggc gggaaaacct ggcgcaaata ctgtcagaaa gtcacggcac
     2041 tcactcacct cactgcacga ctccactact ccatgacacc atcattttct caccgtatcg
     2101 tccgcgcttt gggcgtcgcc gcatgtacgt cccagacaga gttcgactcg atgaacgctc
     2161 acgctcaagg taaacctcgc tcgtgctttg acacgacgaa agagcctgtg aaatactcgc
     2221 aggaaccaat gggaaaggat tagcaaatgg aaatttcaaa gaaaatcgaa tttcatcccg
     2281 attctacggc tttgattatt gttgatatgc aggtggaagg ttgcgagcgg catggacccg
     2341 gtatgaaacc cgtcatcgcc aacatccgcc gcctcctcga tcgctttcgt gcgatcaacg
     2401 gaaagatcat tcatgtgcaa tcggtccgca ccaaggatca tccggagttc accgtatttg
     2461 ggcggccgta cggcttgttg ctgggcagcc cgggggccga tttcgtcgag gaattgaaac
     2521 cgctccccgg cgaggatgtc gtccagaaga cctcgcacga ttgtttctat aagacgtcaa
     2581 tggagccgac tttggagaaa ctggacctcc agccttgccg agacaagatt gtcgtgaccg
     2641 gaatcgggtc gagcaactgc gtttatcatg cggtaatcgg atttcacatc agaaactatt
     2701 acagcatcgt gccggaagac tgcattcacg gcgttcaccc tcagtcccaa ccctttgccc
     2761 tgcatcagtt ccgttccaac gcgtacaatt tcaatgtgac ggtgtcccgg tccgaagatc
     2821 tgcgattgat ttcggatggc gttggtttgg aacgagccgt tagcagatga gcataggatg
     2881 atggacacat tacgctctaa gttgaagatc gtattcatcg ttttcggatt gtttaacata
     2941 cccgtttccg ctatggcgca gagattgaca gtgccgtttg cggcgctgtc tcccacctat
     3001 gcgcctctgt gggtcgccca agaggcggga ttttttaaga agtacggact tgacgtgcag
     3061 gtgatttaca tttcggcagg ttccgtgatt attccagcaa ttttgtctgg tcaggtcgat
     3121 atcgccaata tgagctcggc gccggcgcta accgcgtggt ctcgcggcgc agaactcgcc
     3181 acggtcggcg tgacatcgaa tcgactacta catgtcgtga tggctcgcgg gtcgataaaa
     3241 aagcccgaag atctcaaagg caaaaagatc ggtagcgacc gctatggttc gctctcggat
     3301 ttggtcttgc gtgaagcatt gcggcattac aatatcgcgc cggaccgtga tgtggcggta
     3361 attcaaacgg gtggattgcc ggagcggctt ggtgcgctca agattggagc cgtggatggc
     3421 gcgatcgtta ccggggatac cgctttcgaa gccgagaagc tcggttttca taaagtgatt
     3481 gatctcagcc aactaccgat tcgttatccg ggaagcacta tcgtagtcac gaggtctttt
     3541 ctttccggca agagagagat cgtcaaaaga tttcttcgag gctggattga gggcataaaa
     3601 acagcgaaga ccgacaagga gtttaccatt aaggttatgc aaaagtttct caagaccggc
     3661 gatcgatcca ttctcgacaa gacttt
//
LOCUS       DASUTG010000509         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_818999, whole genome shotgun sequence.
ACCESSION   DASUTG010000509 DASUTG010000000
VERSION     DASUTG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818999"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF089_11665"
     CDS             <1..355
                     /locus_tag="VF089_11665"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6174663.1"
                     /translation="GGRLRKEHFYARCLTVKIRYSDFQTVTRSRTLGSPTCFDREIFE
                     TVKQLLLHNLARDRAVRLLGVSASALQSSGWQESLLDRDKRSSWDKLYQGIDQLRHKY
                     GDEAIGAAGPRKPRS"
     gene            397..1440
                     /gene="hemE"
                     /locus_tag="VF089_11670"
     CDS             397..1440
                     /gene="hemE"
                     /locus_tag="VF089_11670"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6174664.1"
                     /translation="MPNQSIQNHPFLAACRREAVPHTPVWLMRQAGRYMEEYRTLRAQ
                     YGFLELCKKPDLAAEITVTPVERLKVDAAILFADILLILEPMGVGLEYSKGDGPMIHR
                     PVRTGKDVDGLKDFDPEKELSFVYEAVKKTRTALDDRVPLIGFAGAPFTLASYLIEGA
                     GSRNYIHTKKLYYTAPEDWHRLMQRLCQLITKYLNCQIVAGAQAVQLFDSWAGCLTPG
                     DYEEFVLPYTRSVIAGITKGVPVINFSTGTAGLLKQIRATGGDVIGFDWRINLDEGWA
                     MVGHDIAVQGNLDPVALFASPKEIRRRVADVLDRAGNRPGHIFNLGHGVLPETPVDHV
                     IAMVEAVHELSAR"
     gene            1449..2381
                     /gene="hemH"
                     /locus_tag="VF089_11675"
     CDS             1449..2381
                     /gene="hemH"
                     /locus_tag="VF089_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6174665.1"
                     /translation="MVLYQAILLVAFGGPTSSEEIRPFLARVTQGTAIPPQRLEEVAH
                     HYEAVGGKSPLNDITARQAHALQKLLREQGMDLPVYVGMRNAAPFLVDTLRQMAVDGV
                     ERALGFILSSHHTEASWERYQKDVADARLAIGRDAPEIDYCAGWHDHPLFIQTWCELI
                     GESFAGIAREQRSATPLVFTAHSVPVPMANRSPYVEQLEITARAIAEKIGHKNWSIAY
                     QSRTGKPGDAWLEPDINAVLRDLSTHGAAEVVVAPIGFVCDHVEVLYDLDIEARKTAA
                     QAGIKLHRAGCPNDHITFIQMIADVIARSVPAGQ"
     gene            2429..3886
                     /gene="hemG"
                     /locus_tag="VF089_11680"
     CDS             2429..3886
                     /gene="hemG"
                     /locus_tag="VF089_11680"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6174666.1"
                     /translation="MNRRVVVVGGGIAGLAAAHRLIELRKERALSVDVTLLEASHRLG
                     GCIATERFGDFLIEAGPDSFISEKPWALQLCERLGLGPRLVATQSAYQKIYIVHNGKL
                     EPLPEGFFLLAPTQLAPFVRTALFSWRGKLRMVAELLIPRGNANIDESLGAFVRRRLG
                     SEALERVAQPLLGGIYAADPDKLSLISTMPRFKEMENSHRSVIWAMWSEQRRRARKRE
                     GGSGARWSLFVTLAGGMQEMVDAIARRLPQGSIRLNSTVTSIFPSGDKQGWQIATRSN
                     EIFETDGIILATPAFHTAQILASAVPGAATELSSIPYASSATVSLAYRNRDFPRMPDS
                     FGFVVPAIESRKIMACTFSSLKYPGRAPEGHVLLRAFVGGSLQPDLSEDNDPTLERNV
                     RAELASLLGVDAEPQLVRIQRHSNSMPQYHVGHQARIQRIESALDSHGNLALAGSAYH
                     GIGIADCVRSGEEGAEKLFNQIVTTGRDKLTRAKR"
     gene            4094..4942
                     /locus_tag="VF089_11685"
     CDS             4094..4942
                     /locus_tag="VF089_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174667.1"
                     /translation="MTIIDAQVHIWAPETLEKPYAKGEASEPHRPIPLGYEELLREMD
                     GAGVHRCVLVPPTWEADRNDTSLEAARLHPDRFAVMGRLKINAPESRGLVATWNDQPH
                     MLGIRMVFNRGRSREWLDDGTADWFWDAAERYDVPVMTFAPHDVPKLGAIAESHPGLR
                     MIIDHMGLGTALRGKPLDPAVDDVTKMAHVPNVAVKVSALPCYVDEAYPFPTLHPLIR
                     RVVDVFGAQRCFWGTDLSHLPCPYRQAVTLFTEELSFLSETDKEWIMGRGIAEWLHWP
                     LEIGTT"
     gene            complement(4970..5530)
                     /locus_tag="VF089_11690"
     CDS             complement(4970..5530)
                     /locus_tag="VF089_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6174668.1"
                     /translation="MPSLKRAVFTWVILYAVLPAFSSLNAYTTRQYVKLGSFEEASRA
                     IAAAGNDEQSQDLSELVESIHQQVNDFRRAQGRQPLRLNPIISAQARQHSAQMARKGS
                     AVTHVGFEDRLKRIGEQLPYRAGAENVAASIGYENPARTAVVGWKTSPGHRKNMLGDF
                     TLTGIGIAQSGQGSYFITQIFLKASQ"
     gene            complement(5609..6115)
                     /locus_tag="VF089_11695"
     CDS             complement(5609..6115)
                     /locus_tag="VF089_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6174669.1"
                     /translation="MKRKNHILTAAFLAGNMGIGANALFAQALPGPSVPKPVPEIPQQ
                     TQPTIPGQPAPGLPPTEPLPGQRGTIPERMEAPDAGSQREMVISSDDIKKAQEALKAK
                     GKDPGAVSGRMHAKTQEALREFQKENNLPATGVLDKKTADKLGVTLKGDEGSKPQQRQ
                     QGMKPEAK"
     gene            complement(6346..>7317)
                     /locus_tag="VF089_11700"
     CDS             complement(6346..>7317)
                     /locus_tag="VF089_11700"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6174670.1"
                     /translation="AEGFDIRDKPMAPEAVQRCVLLGFCDHVAKRLSPGSQRCEMTHG
                     RRGVLVRESVVKAPLFVVSEAREVESGAAKDRTLNVMLNLATAIKEGWLRELFPRDFS
                     ESRVVVYDPARRRVIARDEVRFRDLLLREKLSDIPPVDEAAAILAREVDTGRCQLENW
                     NQAVEQWILRVNRLRQWMPELELPAIQETDRLSMLEHICHGALSYNEIKGRPVLPLVK
                     SWLSRAQQKLVEEYAPERIQLPGGRTVKVIYSADRPPTIAARIQDLYGIKNALWIASG
                     RVVLRIEVLAPSNRPVQVTENLSTFWNETYPKLKHELRRRYPKHEWR"
BASE COUNT         1486 a         2124 c         2080 g         1627 t
ORIGIN      
        1 tggcggcagg ttgcgcaaag aacactttta cgcccgatgt ctcacggtga agatccgtta
       61 tagcgatttc cagaccgtca cccgctctcg gactttgggt tcgccgacct gcttcgaccg
      121 agaaatcttc gagactgtca agcaactgtt gctccacaat cttgcccggg atcgggccgt
      181 tcggctcctg ggcgtcagcg ccagcgcgct acaatcttcc ggctggcagg aatctctcct
      241 ggatcgggac aagcgcagct cgtgggacaa gctctaccaa ggtatcgatc agctgcgcca
      301 taagtacggc gatgaggcca tcggcgcggc aggcccgcgt aaaccccgta gttgatttca
      361 actgcgaagc tccgtaatct cgtgtagtct tcctcaatgc ccaatcagtc aattcaaaat
      421 catccgttcc ttgccgcatg ccgccgcgaa gcagtgcctc atacgcctgt ctggcttatg
      481 cgtcaggcgg gccggtacat ggaagagtat cgcactctac gcgcgcagta cggctttctt
      541 gagctatgca aaaaacccga tctcgcggcc gaaatcaccg tcacacccgt cgagcggctc
      601 aaggtagacg cggcgatcct tttcgccgac attttgttga tccttgagcc tatgggtgtg
      661 ggattggagt attccaaagg cgatggtccg atgattcacc ggccggtgcg taccggcaaa
      721 gatgtcgatg gcctcaaaga cttcgatcct gagaaggagc tttcgttcgt ctacgaagcc
      781 gtgaagaaaa cccggacggc gctggacgat cgggtgccgc tgatcgggtt tgccggggcg
      841 ccttttacgc tcgcttcgta tctaatcgag ggggccggct cgcgtaacta tatacacaca
      901 aaaaagttgt actacacggc tccggaggac tggcatcggc tgatgcagcg gctttgccag
      961 ctgatcacga aatatctcaa ttgtcagatt gtcgccgggg cgcaggcggt gcaacttttc
     1021 gacagttggg caggttgcct gacgcccggc gattacgaag aattcgttct gccgtataca
     1081 cgctcagtca ttgccgggat taccaagggt gtgccggtga ttaatttcag cacgggcacg
     1141 gctggactgc tgaagcaaat tcgcgccacg ggcggcgacg tgatcggttt cgattggcgg
     1201 attaacctgg atgaaggctg ggcgatggtg gggcatgata tcgcggtgca gggcaacctc
     1261 gatcccgtcg ctttgtttgc atcacccaaa gaaatcaggc gtcgtgtcgc cgacgtgctc
     1321 gaccgcgccg gtaatcggcc cggccatatc ttcaacttgg ggcacggagt gctacctgaa
     1381 acaccggtcg atcacgtgat cgcgatggtc gaagccgtcc acgagttgag cgcgcggtaa
     1441 gctcgtcaat ggttctctat caagctattc tcctggtcgc tttcggcggc ccaacttctt
     1501 ctgaggagat tcggccgttc ctggcgcggg tgacgcaagg gactgcgatt ccgccgcagc
     1561 gtctcgaaga ggtcgcgcat cattatgaag cggtcggcgg caaatcgcct ttgaatgaca
     1621 tcaccgcgcg ccaagcacat gctttgcaaa agttactgcg cgagcaaggc atggatttac
     1681 ccgtgtacgt cggtatgcgc aacgccgccc cgtttctggt cgacaccctc agacaaatgg
     1741 ccgtcgatgg ggttgagcgg gcgttgggat tcatcctctc ctcgcatcac accgaggcga
     1801 gctgggagag gtatcagaag gatgtcgccg atgcgcgttt ggcaatcggc cgtgacgcgc
     1861 ctgagatcga ctactgcgcc ggatggcacg accatccgct gttcatccag acctggtgcg
     1921 agttgattgg agaaagcttt gccgggattg cccgcgaaca acgttcggca actccgctcg
     1981 ttttcaccgc gcacagtgtg ccggtgccga tggctaaccg atcgccgtat gttgagcagc
     2041 ttgagatcac cgcgcgcgcg atcgccgaaa agatcggtca caagaactgg tccatcgctt
     2101 atcagagtcg caccgggaaa cccggcgatg cttggctcga gcccgatatc aacgccgtcc
     2161 tacgtgatct ctcaacccac ggcgctgccg aagtggtcgt cgctcccatc ggctttgttt
     2221 gcgatcatgt cgaagttctt tacgatctcg acattgaagc gaggaaaaca gcggcacagg
     2281 ccggcatcaa attacaccgc gccggttgcc cgaatgatca cataactttt attcaaatga
     2341 ttgcggacgt gattgccaga tccgtcccgg ccggtcaata aatgcgaact tcaagacgat
     2401 tgactggctt ctccgagaga cgccgcgtat gaaccgtcgc gtcgtcgtcg tcggcggcgg
     2461 catcgccgga ttggcggcgg cgcatcgcct gatcgagtta cgaaaagagc gggccttaag
     2521 cgtcgatgtc actctgcttg aggcgtccca tcgtctcggt ggctgcattg cgacggagcg
     2581 attcggcgat tttcttatcg aagccggacc ggattcgttc atctctgaaa agccttgggc
     2641 gctgcagctc tgtgaacggc tcggattggg accgcgtttg gtggcgaccc agtctgccta
     2701 tcagaagatc tacatcgtgc ataacggcaa gctcgagccg ctgcccgaag gttttttctt
     2761 actggcgccg acccaactgg ctcccttcgt tcgaacggcg ctgttttctt ggcgcggaaa
     2821 actgcgcatg gtcgctgaac tgctcattcc gcgcggcaac gccaacatcg atgaaagtct
     2881 cggcgccttt gtccgccgcc gccttggcag cgaggccttg gagcgagtcg cccagccgct
     2941 gcttggcgga atttacgccg ccgatcccga caaactcagt ctcatctcca ccatgccgcg
     3001 cttcaaagag atggaaaatt ctcaccgcag cgtcatttgg gcaatgtggt cggagcagcg
     3061 gcgtcgtgca cgcaagcgcg aaggcggcag cggcgcgcgt tggagcctat tcgttactct
     3121 tgccggcggc atgcaggaaa tggtcgatgc gattgcccga cgtttgccgc aaggaagcat
     3181 tcgcttaaac agcactgtga caagtatatt tcccagcggc gataaacagg gctggcagat
     3241 cgcgaccagg agcaatgaaa ttttcgaaac cgacggcatc attttggcca ctccggcgtt
     3301 tcacactgca cagattctgg cgtcagccgt ccctggagca gccacggagt tgtcgagcat
     3361 cccttacgcc tccagcgcaa ccgtaagtct ggcttatcgt aatcgggact ttccacgtat
     3421 gcccgacagc ttcggcttcg tcgtgccggc gattgaatcg cgcaaaatca tggcgtgcac
     3481 tttcagcagt ttgaaatatc ccggcagagc cccggaaggc catgtgctat tgcgcgcctt
     3541 cgtcggcggc agcctgcaac cggatctttc ggaggacaac gatccgactc tggaacgaaa
     3601 cgtccgagcc gagctagcca gtctgctcgg cgtcgacgcc gaaccgcagt tggttcgaat
     3661 ccagcgccat agcaactcta tgccgcaata tcacgtcggc catcaggcca ggattcaacg
     3721 gatcgaatca gctctcgaca gtcacggcaa tctcgcgctc gccggcagcg cgtaccacgg
     3781 catcggtatt gccgattgcg tgcgcagcgg cgaagagggc gcggaaaagt tattcaacca
     3841 gatagttacc accggtcgag acaaactcac ccgtgcaaag cgctgatctg aaacaatcgc
     3901 atgaacctac cgataatcct gtgacgattg ccataggagc gcagcaggtt ccctcgccct
     3961 ccggcagaca cggaaaagga tttgtgattg ccagcggcgg gagaaatctt ttccttgacg
     4021 gttgccgatc gccagcctat gaattgcgta acgaagctgt cgttcccctg caagatggag
     4081 tagcagatcg cccatgacaa tcattgatgc tcaggtccat atctgggcgc ccgaaacgct
     4141 tgagaagccc tatgcaaaag gtgaggcatc cgagccacat cgccctatac ccctcggcta
     4201 cgaggaacta ttacgggaga tggacggcgc cggagtgcac cggtgtgttc tcgtgccgcc
     4261 gacgtgggaa gcggatcgca acgacacctc gttggaagcg gcgcgtttac atcccgaccg
     4321 ttttgcggtc atgggacggc ttaaaatcaa cgcgccggaa agccgcgggt tggtggcgac
     4381 atggaatgac cagccccaca tgctgggcat taggatggtc ttcaatcgcg gacgatctag
     4441 agaatggttg gacgatggca ccgcggattg gttttgggat gctgcggaac gctatgatgt
     4501 acctgtcatg accttcgcgc cccatgacgt gccgaaactg ggtgcgatcg cagaaagcca
     4561 tccgggattg cgcatgatca tcgatcatat gggacttggt acggctttgc gcggtaaacc
     4621 gttggatcca gccgtggacg acgtgaccaa gatggcgcac gtgcccaatg tcgcggtaaa
     4681 agtttctgcg ctgccctgct atgtggacga agcttaccca ttcccgactc ttcacccgct
     4741 gattcgccgg gtcgttgacg tcttcggcgc gcagcgctgc ttttggggca cagacctatc
     4801 tcatctgcct tgcccttatc gacaagcggt gaccttgttc actgaagagc tgagttttct
     4861 ctcagagaca gacaaggaat ggatcatggg ccgcggcatc gccgagtggc tccactggcc
     4921 gcttgaaatc ggcacaacgt gaatgcgcct tcgctactcc gccgtacctt tactgtgatg
     4981 ctttcaagaa gatttgtgtg atgaaataac tgccttgccc gctttgcgcg ataccgatgc
     5041 cggttagagt aaagtcaccg agcatatttt tgcggtgccc cgggctagtc ttccatccaa
     5101 ccactgccgt tcgcgccggg ttttcataac cgatactggc cgcgacattt tccgcgcccg
     5161 ctcggtaagg aagctgctcg ccgatccttt ttagccgatc ctcaaaccct acatgagtca
     5221 cggcgcttcc cttacgggcc atttgcgcgc tgtgctgtct tgcttgcgcg ctgatgatcg
     5281 gattaagccg tagcggttgc cggccctggg cacggcgaaa gtcgttcact tgttgatgga
     5341 tgctctcgac gagctccgac aagtcttgcg actgctcatc gttccctgct gcagctatcg
     5401 cccgtgatgc ttcttcgaag gatccgagtt ttacgtattg gcgggttgtg tatgcgttga
     5461 gactcgagaa cgccgggaga acagcataca ggatcaccca ggtaaagact gcccgcttaa
     5521 gtgaaggcac gaatataccc cagattgtga gatcgggaag acgcggtttg cgccctcccg
     5581 atctggatgg gtggaaacta aacgctactt acttggcctc tggcttcata ccctgctgcc
     5641 gctgctgcgg cttcgaacct tcgtcgccct tcagggtcac accgagttta tcggcggttt
     5701 tcttgtcaag cactccggta gctggaagat tgttttcttt ttgaaactca cggagcgctt
     5761 cttgagtctt cgcatgcata cggccgctga ccgcccccgg gtctttcccc ttcgccttta
     5821 atgcttcctg tgccttcttg atgtcatcag aggagatcac catctcgcgc tggctgccgg
     5881 catccggcgc ctccatccgt tccggtatag ttccacgctg tcctgggagg ggttccgttg
     5941 gtggaagccc tggcgccggt tgccccggta tggtcggctg tgtttgctgc ggaatttccg
     6001 gtaccggttt cggaacgctc ggtccaggaa gggcctgggc gaacagagca ttcgcgccta
     6061 tgcccatatt accagctaaa aaagccgctg tcagaatatg gttttttcgt ttcatggaga
     6121 gcctcctttt acgatcttgc tctattaggg gcgcaagccg catgccacga aaagagcgcc
     6181 gccaaactag tggaagagtc ggaagaaggt atctagaagg aagaatctca tagttcggtg
     6241 tatcgtattt gagaagacga ctttgtctac gtccgaatag agcgactttc tgatcacgcg
     6301 ccggaccgcg gtatgttgct actgatatac attccatcga ggtcttcagc gccattcgtg
     6361 ctttggatac ctgcgccgca actcgtgttt aagtttcggg taggtttcat tccagaaagt
     6421 cgacaggttc tcagtgacct gcaccggacg attgctgggc gcgagcacct cgatccgcag
     6481 gacaacacgc ccgctggcaa tccaaagcgc gtttttaatg ccatacagat cctgaatgcg
     6541 cgcggcaatc gtcggcggcc ggtcggcgga gtagatcact ttcaccgtcc ggcctccggg
     6601 taactgaatg cgctccggcg catactcctc gaccaacttc tgttgtgcgc gtgacagcca
     6661 tgatttgacc agcggcaaaa caggtcggcc ttttatttcg ttgtagctga gcgcgccgtg
     6721 gcagatgtgc tcgagcatgg agaggcggtc cgtctcttga attgccggca gttcgagttc
     6781 cggcatccat tgccgcaatc ggttgacgcg caggatccat tgctcaaccg cttgattcca
     6841 gttctcgagc tggcagcggc cggtgtcaac ttcccgcgct agaatcgccg ccgcctcatc
     6901 gacgggtggt atatcggaga gcttctctcg tagaagcaga tcacggaatc ggacctcgtc
     6961 ccgcgcgatc acgcgccgcc gcgccggatc gtaaacaaca acgcggcttt cgctaaagtc
     7021 ccgaggaaac agctcgcgca accacccctc cttgatggcg gtagccagat tcagcataac
     7081 gttcaacgtc cgatccttag cagcgccgct ttccacctcg cgcgcttccg atacgacaaa
     7141 aagtggagct ttgacaacac tctctcggac caatacacca cgccggccgt gcgtcatttc
     7201 gcaccgctgc gagccgggac taagacgttt ggcgacatgg tcacaaaatc caagcagcac
     7261 gcaacgctgc accgcctccg gcgccatcgg cttgtcgcgg atgtcgaaac cttcggc
//
LOCUS       DASUTG010000510         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1263469, whole genome shotgun sequence.
ACCESSION   DASUTG010000510 DASUTG010000000
VERSION     DASUTG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263469"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="argJ"
                     /locus_tag="VF089_11705"
     CDS             <1..948
                     /gene="argJ"
                     /locus_tag="VF089_11705"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX6174671.1"
                     /translation="KNANVAMGKQGIANAIETCRIVGKELGISPYDVLPFSTGVIGRP
                     LPMDKIRAGLKGIRNELKPDNLKLAAEAIMTTDMYPKYIFCQVGSAVIAGIAKGAGMI
                     EPNMATMLVYLMTDAELPRSTMRPMLRRVVDRTFNCMSIDTDTSTSDTVVLMANGLAG
                     KVNLGHFEDALSHVCEFLTREIARNGEGATKLLIVDVSGSKSGAQANLVAKSIVNSPL
                     VKTAVYGCDPNWGRVIMAVGKTFDPVIDPAKVTIRFGDTAVFKKGSPVDCDLEGLRDY
                     LGQAEVTISVDLGVGKTSARVWGCDLTEGYIKENAYYTT"
     gene            complement(1296..3326)
                     /locus_tag="VF089_11710"
     CDS             complement(1296..3326)
                     /locus_tag="VF089_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6174672.1"
                     /translation="MVKKIPAELEKHGHVRVDDYYWLKDRNNPEVIAYLKAENEYSDR
                     IMVHTKPLEEKIFEEIKSRIAQTDMSVPYRREGYLYYTRYEQGKEYPIFARRKGSMDE
                     PEQIMLDANLLAQGHDFFSIGGWALSADQNLMAYAVDTQGRRIFTVYIKDLITGELLP
                     DVIPNVTENLTWADDNHTLFYAKQDLKTLRAHQIYRHRLGTSAESDQLVFEESDETYA
                     AYVYKTKSKKYLIIVSAQTLSHEYRYLEADDPFGEFKIFLPRQREHEYSIDHYADRFV
                     IRTNDEARNFRLMVTPVKKIAKEHWQEIVPHRQDVYLGDFEIFRDYLVLEERNRGLTQ
                     IRVIPWSGGQGYYLPFDEPAYRANIGVNPEFDTPVLRYEYTSMKTPLTIYDYDMVTHE
                     RTLLKQEQVLGGFDSSNYVSERLHTTAPDGVEVPISLVYRKGLERDGDSPLLLYGYGS
                     YGASIDPAFSSPRLSLIDRGFIFAIAHVRGGQELERQWYDDGRLLKKKNTFTDFIACA
                     EYLIREKYTRPEKLFAMGRSAGGLLMGAVTNMRPDLFKGIVAEVPFVDVITTMLDPNI
                     PLTTGEYDEWGDPNQKDVYEYMLSYSPYDNVEAKQYPNLLATGGLHDSQVQYWEPAKW
                     VAKLRDLKTDNNRVLLKTNMEAGHGGSSGRFRRHRETAFSYVFLLDLAGIKE"
     gene            3524..>4020
                     /locus_tag="VF089_11715"
     CDS             3524..>4020
                     /locus_tag="VF089_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6174673.1"
                     /translation="MALACAILASAVPHATHAQTAKAIVLAWDGTVSTFVDDLVREGK
                     LPNLAKLIAGGAFADDVIPGFPSKTAPGFASLMTGAPPRITGISGNRVPREPRADYTI
                     LDSIAGFSAAPLLADPLWAAAQRAGKNVVISHIPAFAAENAENTVRFYGYNMIAGRDG
                     VVTPR"
BASE COUNT          924 a         1097 c         1056 g          943 t
ORIGIN      
        1 aagaacgcca acgtcgccat gggaaaacag ggcatcgcca atgccatcga aacctgccgg
       61 attgttggca aagagctcgg cataagtccc tatgacgtcc tgcctttttc taccggcgtc
      121 attggccggc ccctgccgat ggacaaaatc cgcgccggat tgaaaggcat tcgcaacgaa
      181 ctcaagcctg acaatctgaa gttggccgcc gaagccatca tgactacgga catgtatcca
      241 aagtacatct tctgccaggt cggttcggca gtcatcgccg gaatcgccaa aggcgccggt
      301 atgatcgagc cgaatatggc gaccatgctg gtctatctta tgaccgacgc ggaactgccg
      361 agatcgacga tgcgccccat gctgcgccgc gttgtcgacc gaactttcaa ttgcatgagc
      421 atcgataccg ataccagcac cagcgataca gtcgtgctga tggcaaacgg tttggccggc
      481 aaagttaact tggggcattt tgaagatgcc ctatcgcatg tttgcgaatt tctcactcgc
      541 gagatcgccc ggaacggcga gggcgcgacc aaacttctca ttgtcgatgt cagcgggtcc
      601 aaaagcggcg ctcaagcaaa tttggtcgcc aagtccatcg taaactcgcc cttggtcaaa
      661 acggctgttt atggatgcga cccgaactgg ggccgtgtga tcatggcagt gggcaaaact
      721 ttcgatcccg tcatcgatcc tgccaaagtc accatccgct tcggcgacac ggcggttttc
      781 aagaaaggct cccccgtcga ttgcgatctc gaaggtttgc gcgactatct tggtcaagcg
      841 gaggtcacga tctcggtgga tcttggcgtc ggcaaaactt cagctcgcgt atggggttgc
      901 gatttgaccg agggatacat caaagaaaat gcctactaca cgacctgatt ttttttacaa
      961 ttcgtgtggc gatcgaattt gatcatttgg gctagtgccc tccaacgatt caatgagacg
     1021 aggcaatcaa gggacggcat tccgtttcgt tcatagagac aacacccctg acttcacgag
     1081 agcgaccaac cgacttccgc tcacagagct gacgcttggg aattctccta gtagcgcgct
     1141 gaaaaaagcc tcttgcgggc tgctcaaaaa gatccaggtg cgaggcgcgc gaaaaatcga
     1201 cgagcggagc cgtacttaca gtacgtcgca gcgaggcgat tgagcgcaac gaagtatatg
     1261 agtctttttt caacagcctg ctagaacgaa aacggctact cctttatgcc ggcgaggtcg
     1321 aggagaaaga cataggaaaa agcggtctcg cgatggcgcc ggaatcgccc actggacccg
     1381 ccgtgaccgg cttccatatt ggttttaagc agcacccggt tgttgtcggt ctttagatcg
     1441 cgcagcttgg ccacccattt cgccggctcc caatactgca cttgggaatc gtgcaagccg
     1501 ccggtggcta acaggttggg atactgctta gcctccacgt tgtcgtaagg ggagtaggag
     1561 agcatgtact cataaacatc cttctgattg ggatcacccc attcatcgta ttctccggtg
     1621 gtcagcggaa tattaggatc gagcatcgtt gtgatcacat cgacaaacgg cacttcggca
     1681 acaattccct tgaacagatc cggccgcatg ttggtgacag cgcccatcaa caggccaccg
     1741 gcgctgcggc ccattgcgaa cagcttttcc ggacgggtgt atttctcgcg gattaaatat
     1801 tcggcgcaag cgatgaagtc ggtaaaggta tttttctttt tcaaaagtct gccgtcgtca
     1861 taccactgcc gctccagctc ttgaccgccg cggacgtggg cgatggcaaa aataaatccg
     1921 cgatcgatca ggctcaaacg tggcgaagaa aatgcaggat cgatgctggc gccatacgag
     1981 ccgtaaccgt agaggagcag cggactgtca ccgtctcgct ccaatccctt gcgatagacc
     2041 agagaaatcg gcacctcaac gccatccggc gccgttgtat gcagccgctc tgaaacgtaa
     2101 ttacttgagt cgaaaccgcc caatacctgt tcttgtttca gcaacgtccg ttcgtgcgtg
     2161 accatgtcgt aatcataaat cgttaacggc gtcttcatcg aagtgtactc gtaacgcagg
     2221 acgggtgtgt caaactccgg attgactcca atattggcgc gatacgccgg ctcatcgaac
     2281 ggaagatagt agccttgtcc gccggaccag ggtatcacgc ggatctgggt cagcccccgg
     2341 ttacgctcct ccaacaccag ataatcccta aaaatctcga agtcgccgag atagacgtct
     2401 tgccgatgcg gaacgatctc ttgccaatgc tctttggcga tttttttcac cggcgtgacc
     2461 atgagacgaa aattcctggc ctcatcattg gtgcgtatca cgaaacgatc ggcgtagtga
     2521 tcgattgaat attcgtgttc gcgctgccgc ggcaaaaaga ttttgaactc accaaacgga
     2581 tcgtcagcct caagataacg gtattcgtgg ctgagggttt gtgccgaaac aatgatgaga
     2641 tactttttcg attttgtctt gtagacataa gccgcataag tttcatcgga ttcctcaaac
     2701 accagctgat cgctttccgc ggaggttccg agtctgtggc ggtaaatctg atgggcgcgc
     2761 aaggtcttta ggtcctgctt ggcataaaaa agcgtgtggt tatcgtcggc ccaagtgaga
     2821 ttttccgtaa cgttgggaat gacgtccggc aacagttccc cggttataag atctttgatg
     2881 taaaccgtga agatccttcg gccctgcgta tccaccgcat acgccatcag attttgatcg
     2941 gcactgaggg cccagccgcc gatggaaaaa aaatcgtgac cctgtgccag caggttggca
     3001 tcgagcatga tctgttcggg ctcatccatc gaaccttttc tgcgtgcgaa aatcgggtat
     3061 tcttttccct gttcgtagcg ggtgtaatac aaataacctt cccgtctgta gggaaccgac
     3121 atgtccgttt gggcaattcg ggatttgatt tcttcgaaga tcttctcctc caatggcttg
     3181 gtatgaacca tgatccgatc ggaatactcg ttctcggctt tcaggtaagc gatgacttcg
     3241 ggattgttcc gatcttttag ccagtagtaa tcgtcgacac gcacgtggcc gtgtttttcc
     3301 agctcagcgg gaattttttt caccatcgac tgcctttccg gactccccca ggcccgcaaa
     3361 agaatggagt atccccgggg aggtaccagc attgattatg aaataatcac cgcagccgca
     3421 accggctccc aggttctcca agcaatctga tgagcgcatc acattgacag ctggttttca
     3481 catcgctact ctcatcattt cgatgcatgg tcgttttcgt atagtggcat tagcatgtgc
     3541 gatcctcgca tccgccgttc cccatgcgac ccacgcccag acagctaagg ctatcgtgct
     3601 tgcatgggac ggcacggtat caacctttgt cgatgattta gttcgggaag gaaagctgcc
     3661 caatttggcc aaactcattg ccggcggtgc ttttgcggac gatgtaatac ctggatttcc
     3721 gtcaaagacc gcgccgggtt ttgcctccct gatgaccggc gcgccgccgc ggatcactgg
     3781 gattagcggc aatcgagtgc cgcgcgaacc ccgcgccgat tacacgatcc ttgacagcat
     3841 cgccgggttt tcggcggcgc cgttgctagc ggacccctta tgggctgcgg cccaacgcgc
     3901 cggcaaaaat gtggtgatct cgcacattcc tgcctttgca gcggaaaatg cagagaacac
     3961 cgtacgattc tacggctaca atatgatcgc cggacgggat ggcgtcgtca caccgcggtt
//
LOCUS       DASUTG010000511         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_375236, whole genome shotgun sequence.
ACCESSION   DASUTG010000511 DASUTG010000000
VERSION     DASUTG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375236"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF089_11720"
     CDS             complement(<1..423)
                     /locus_tag="VF089_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6174674.1"
                     /translation="MTREITLRLNGRRERFTIDDADTLLEVLRDRFKLWSVREGCGVG
                     ACGTCTVLLDDKPVSSCFLLAARAAEHDIVTLEGLGDGGTLHYIQEAFVSQRALQCAY
                     CTPGFVLSVKAMLDENPDPSDEDVREYLSGNLCRCAGYA"
     gene            complement(429..2756)
                     /locus_tag="VF089_11725"
     CDS             complement(429..2756)
                     /locus_tag="VF089_11725"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6174675.1"
                     /translation="MANFAVVGQSVFRKDASQKVTGQVLHVGNIELAGMLHVAVLRSP
                     YAHARITRIDKTKAEAVNGVAVVLTGADIAKMPGVDPHFGPAFRDQPILAVEKVCYTG
                     DPVVAVAAQDRRTAEDALELVEVEYEPLSPVLDVLEAVKPGSPLVHEQHRPAKAFADL
                     AHVKSGQQSNICYHFKLRTGDVDKAFAEADRVFEDTFSSPPAQHMPMEPHVTLVYLDE
                     GQRINIWTASQTPSYVRTEIANTFGVPMNRIRVRIPYLGGGYGAKLYAKLEPLVTALA
                     LVTKKPVRYALTREEEFLTITKHKVITRIKTAIKDGVITARKCEVFWDTGAYAEIGPR
                     VVHKSGYTSAGPYRIPNVWIDSYCVYTNHVPAGAFRGFGVPQVIWAYDSQTDTIARAI
                     GADPVEFRLQHALNEGEAFATGTPVKSFGIKQAVREAADAIEWSKPRQKDSGTKLRGK
                     GIAAGVKAVLTPSISGAIVIMNADGTVNVLSSTVEMGQGSETMMGQIVAEELGVSFDQ
                     VHVVQPDTDITPYDTITAGSRSTYHMGNAVRMAAGKVKAELFQVVSKKLEVNPDDLAA
                     GGGRIFVRGTEERGMTISEAFLAKFGSLGTTLTGEAICQPEAIPMDPETGQSEKCTEY
                     WFPSATSAEVEVDTETGHVKILQFYSVGDTGTAINPKHCEQQLLGSAITHLGLTLFEE
                     MIFDEGQLVNGSLLDYQIASFKDLPESFRPIIVEVPHETGPFGAKGVGETGTLTVSAA
                     VANAVEDAVGVRIRDLPITPEKVLRALSQRQADRG"
     gene            complement(2761..3633)
                     /locus_tag="VF089_11730"
     CDS             complement(2761..3633)
                     /locus_tag="VF089_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6174676.1"
                     /translation="MTIRSFELLQPRSVDEAIELLAKHGDEARPIGGGTTLIILMKQR
                     ALHYPYLVDLQSIAGLSGMSSESDGVRIGALATHRSVELSPLIRQAFPLVAEAFSKIG
                     NVRVRQTASVGGNLAHADYRLDPPPPLMVLGTVVNAVGPNGSRTIPIKDFFRGLYETA
                     LEPGELIVDVKIPFMPPDSEAVYLRYSSLSGNDWPCLGVAALAIRQNGRCQELRLALG
                     GVAATPLLIEGLDLVKDQSLDESVIEKLLGVVDEQISPSSDMRGSEWYKREMVRVFVK
                     KAILQLDDPVAASR"
     gene            3848..4660
                     /locus_tag="VF089_11735"
     CDS             3848..4660
                     /locus_tag="VF089_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174677.1"
                     /translation="MFATIRHIAVMTENWDRMAKFYQTIFDMKKITNGMTDETGTYNK
                     DRGHLSDGVIGLALLQRQPGFRSGLDHFGFAVDDVPLVRDRLKQHYPDIYVAQSQSHV
                     PFAGLRTHDPDGNQFDLSQKGMDNVREGYVEFGWEQPRWINHICIRSGRPAYIAEYYQ
                     KVFDLRPVEGLSGDNSYYLSDGKVSLAIRPWDMVAYRGLMAGLDHFGFKVDDLDKTKK
                     DLEALAATESKSGARKIAIGRDGEVRQKNLEACKLCKYAISDPDGVLLDLTD"
     gene            4795..>5479
                     /locus_tag="VF089_11740"
     CDS             4795..>5479
                     /locus_tag="VF089_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6174678.1"
                     /translation="MSRLSGIISAHVATSLKQLIDSYDSKVLLSEASTPPCSWYSDPR
                     ILDLERQTVFSRSWQMAGRAEQLREPGQYVTSDIAGEPILLLMGHDGVVRGFYNVCRH
                     HAAAVITEPQGRAEHLRCPYHGWTYNLDGTLVVAPEFGGVSNFDKSANGLVPVETAIW
                     QGWVFVKLISNGTSLEAFLGKDLTERIQSSKLEQLSWFDRRFYSVNCNWKVFIDNYLD
                     GGYHVPHIHG"
BASE COUNT         1193 a         1566 c         1510 g         1210 t
ORIGIN      
        1 ggcgtacccg gcgcagcggc acaggttacc agagagatat tcacgaacat cttcatcgga
       61 agggtccgga ttctcatcga gcatcgcttt gacggataga acgaaaccgg gagtgcagta
      121 ggcgcactgt agtgctctct gactgacgaa tgcttcctga atgtaatgaa gcgtcccgcc
      181 atcgccgaga ccttccaggg tcacgatgtc atgctcggct gcgcgggcgg ccaatagaaa
      241 gcacgagctt accggtttat catcgagcaa cacggtgcag gtgccgcacg cgccaacgcc
      301 gcagccttcg cgcacactcc ataatttgaa gcgatcgcgc agtacctcga gcaaagtatc
      361 ggcgtcatcg atagtaaacc tttcccggcg gccgttgaga cgaagagtta tttcacgtgt
      421 cattcgatct aacctcgatc agcttgcctt tgagaaagcg cgcgcaaaac cttttccggc
      481 gtgattggca gatcgcgaat gcgaacgccg accgcatcct cgacggcgtt ggcgacagcc
      541 gccgagactg tgagcgtgcc ggtttcgcca acgcccttgg cgccgaacgg accggtttcg
      601 tgcggcacct cgacgataat cggccgaaaa gactccggca gatccttgaa agaagcgatt
      661 tggtaatcga gcaaagaacc gttgaccagc tgtccttcat cgaaaatcat ttcttcgaaa
      721 agcgtcaagc ctaaatgagt gatcgccgag ccgagcagtt gctgctcgca gtgcttcgga
      781 ttgatcgccg tgccggtatc gccgacgcta tagaattgaa gaattttgac gtggccggtc
      841 tcggtatcga cctcgacctc agccgacgtc gccgagggaa accaatactc ggtgcatttc
      901 tccgactgcc ccgtttccgg gtccatggga atggcctcgg gctggcagat cgcctcaccg
      961 gtaagtgtcg tgccgaggct gccgaacttc gcaagaaacg cctcgctaat cgtcatgccc
     1021 cgctcttccg tgccgcgcac gaaaatacgc ccgccaccgg cggctaaatc atcggggttc
     1081 acttccagtt tcttcgagac gacttgaaac agttcggctt tgaccttgcc ggcggccatg
     1141 cggacggcat tgcccatgtg ataagtcgag cgactgcccg cggtgatggt atcgtatgga
     1201 gtgatatcgg tgtcgggttg cacgacatgc acctgatcga atgaaactcc aagctcttcg
     1261 gcgacgatct gccccatcat cgtctccgat ccctggccca tctccaccgt gctgctgagc
     1321 acgttcacgg tgccgtcggc attcatgatc acgatggcgc cggagattga aggtgtgagc
     1381 accgctttta cccccgccgc aatcccttta ccgcgcagtt tggtgcccga atctttctga
     1441 cgcggcttgg accattcgat ggcatctgcg gcttcgcgca ccgcctgctt gatgccgaac
     1501 gatttcaccg gcgtcccagt ggcgaaggcc tcgccttcgt tcaacgcgtg ttgcaggcga
     1561 aattccaccg gatcggcgcc gatggcgcgt gcgattgtgt cggtttgcga atcgtaagcc
     1621 cagatcacct gcggcacacc aaagccacga aacgcgccgg cgggcacgtg attggtgtac
     1681 acgcagtacg aatcgatcca gacattggga atccgatagg ggccggccga ggtatagccc
     1741 gatttgtgca caacccgagg gccgatctcg gcgtacgcgc cggtgtccca gaagacttcg
     1801 catttgcgcg ccgtgatcac accgtcctta atcgcggtct taattctggt gatcactttg
     1861 tgtttggtga tcgtgagaaa ttcttcttcg cgggtcaaag catagcgaac gggcttcttg
     1921 gtgaccaatg ccagggcggt caccagcggc tccaatttag cgtagagctt ggcgccgtaa
     1981 ccgccgccga ggtaaggaat tcgcacgcga atgcgattca tcggcacgcc gaaggtgttg
     2041 gcgatttctg tgcgcacata cgacggtgtc tggctggcgg tccagatatt tatgcgctgg
     2101 ccctcgtcca gataaaccag ggtgacgtgc ggttccatcg gcatgtgctg cgccggcggc
     2161 gagctaaacg tgtcttcgaa tacccggtcg gcttcggcaa acgctttgtc gacatctccg
     2221 gttctaagct tgaagtgata gcagatattc gattgctggc cggattttac atgggcaaga
     2281 tcggcaaaag ccttggccgg tctatgttgc tcgtgaacta ggggcgagcc gggtttgacc
     2341 gcctctagaa catcgagcac gggagacaat ggttcatact cgacttcaac cagctcgagg
     2401 gcgtcttccg ccgtgcgccg gtcctgcgcc gcaaccgcca ccactggatc gccggtataa
     2461 cagacctttt cgacggcgag aatcggttga tcccgaaacg ccgggccgaa atgcggatcc
     2521 acgcccggca tcttggcgat atcagcgccg gtcagaacca ccgccactcc gttgactgcc
     2581 tcggctttag tcttgtcgat gcgcgtgata cgcgcatgcg cataggggct gcgcaaaacc
     2641 gccacatgca gcatacccgc cagttcgatg ttgccgacat gcaatacctg tccggtcacc
     2701 ttttgacttg catctttgcg gaagactgat tggcctacga ctgcgaaatt cgccattcga
     2761 tcaccttgac gccgcgaccg gatcgtccaa ctgtaagatc gcttttttga caaacacacg
     2821 aaccatctcg cgcttgtacc actccgagcc gcgcatgtcg gaggaaggcg atatttgctc
     2881 gtcgacaacg ccgagcaatt tctcgatgac gctctcatcg agtgactgat cctttaccag
     2941 atccaatcct tcgatgagca gcggtgtcgc agcaacgccg cctaaggcca atctgagctc
     3001 ctgacaacga ccgttttgtc ggattgccag cgccgcaact cccaaacacg gccaatcatt
     3061 gccggacaac gagctgtacc tgagataaac cgcttcgcta tccggcggca taaacggaat
     3121 tttcacatcg acgataagct cgccgggttc caatgcggtt tcgtagagtc cacgaaagaa
     3181 atctttgata ggtatggttc tcgaaccgtt agggcccacg gcgttgacca ccgtgccgag
     3241 aaccattagc ggcggaggcg ggtcgagacg gtaatcggca tgagcgagat tgccgcccac
     3301 cgaggcggtt tgtctgaccc ggacattgcc tattttgctg aacgcctccg cgaccagcgg
     3361 aaatgcctgg cgaatcagcg gcgagagctc gacactccga tgtgtcgcca gcgcgccgat
     3421 tctcacgccg tcgctttcac tggacattcc acttagccct gcgattgatt gcagatccac
     3481 caggtaggga tagtgcagcg cccgctgctt catcaaaatg atcaaagttg taccgccccc
     3541 aatgggccgg gcctcatcgc cgtgcttggc gagcaattcg atggcttcat caaccgaacg
     3601 cggttgtaac aattcaaatg accggatcgt cattcttgtt cctctagccc agtgctgttt
     3661 ctagcctcgg cgatccgaat aagtcaaggc gactgggttg ttcaccgcgg aaatgcggag
     3721 gacgtggagc cggaaagttt aggaattaaa ctctgcgcac tctgtgcctc tgcggtgaaa
     3781 gcaatccgaa tggttgacag gcacagccag gtttgagaga actcggtcaa ctaggagggc
     3841 catgatcatg tttgcaacca tcagacacat tgccgtcatg actgagaatt gggatcgcat
     3901 ggcgaaattc taccagacga ttttcgacat gaagaaaatt accaatggca tgaccgacga
     3961 aaccggcact tacaacaaag accggggtca tctaagcgac ggcgtcatcg gcttggcttt
     4021 gttgcagcgc cagcccgggt ttagatcggg cctcgatcat tttggcttcg ccgtagacga
     4081 tgtgccgtta gtgcgtgacc gtttgaagca gcactatccc gatatctatg tcgcgcaaag
     4141 ccagagccac gttcccttcg ccggtttgcg aacccatgat cccgatggca accaattcga
     4201 tctgtcgcaa aaaggcatgg acaacgttcg cgaaggctat gtcgaatttg gctgggaaca
     4261 accgcgctgg attaaccata tatgcatccg cagcggaagg cccgcttata ttgccgagta
     4321 ttaccaaaag gttttcgact tgcggccggt ggaaggcctg tccggcgata acagctacta
     4381 cttgagcgac ggcaaggtga gtttggcaat tcggccctgg gacatggtgg cttatcgggg
     4441 gttgatggcc ggtttggatc attttggttt caaggttgac gacttggaca aaactaaaaa
     4501 agatctcgaa gcgctcgccg ccaccgagtc caagtcgggc gcccggaaaa tcgccattgg
     4561 ccgcgatggc gaggtgcggc agaaaaattt ggaagcctgc aagctctgca agtacgccat
     4621 ctccgatccc gatggagtat tgctggatct gacggactaa attcatccaa gccgggacat
     4681 acccatcgga attccggtcc aggccttgtt ctgaagtgag ttcgctatgc gaacgcgggc
     4741 tgacggaaca acacaccgct cacggcatat cggcatgaaa aatttcactc aacgatgagc
     4801 cgcctttcgg gcataatctc tgctcacgtg gcaacttctt taaagcagct aattgacagc
     4861 tatgattcca aggtactctt gagcgaagct tccacgcccc cttgttcctg gtattccgat
     4921 ccgcgcattc tcgatttaga gcgacagacg gtcttttccc gctcatggca aatggccgga
     4981 cgcgcggagc aacttcgcga accgggccag tacgtcacgt ccgatattgc cggggaaccg
     5041 attttgctcc tcatgggcca tgacggcgtt gtacggggct tctacaatgt ttgtcgccac
     5101 catgccgcgg ctgtgatcac ggagcctcaa ggccgcgccg agcatcttcg ctgtccctat
     5161 catggttgga cctataacct ggacggcacg ctggtcgtgg caccggagtt cggcggcgta
     5221 agtaatttcg acaaatctgc caacgggctg gtgccggttg aaactgcaat ctggcaaggc
     5281 tgggtattcg taaaactcat ctcaaacggc acgtcacttg aagcattcct cggcaaagat
     5341 cttactgaac gaattcaaag ttctaagctt gagcaacttt cttggttcga tcggcgcttc
     5401 tattccgtca actgcaattg gaaagttttc atcgacaact atctcgatgg cggctatcac
     5461 gtgccgcaca ttcatggcg
//
LOCUS       DASUTG010000512        12559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1709653, whole genome shotgun sequence.
ACCESSION   DASUTG010000512 DASUTG010000000
VERSION     DASUTG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709653"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VF089_11745"
     CDS             <1..435
                     /locus_tag="VF089_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6174679.1"
                     /translation="NYVNAPILAKERGIQVVESKSSKAGDFSSSITVKIATADHEVEV
                     EGAIFGDKHPRIVRVDNFYLEAAPEGYILILRNKDVPGVVGNVGTILGKNGINIAGME
                     LGRGAKGGNAISFIHVDDLIPKETMKELRSLPQTVSAELVKL"
     gene            453..1748
                     /locus_tag="VF089_11750"
     CDS             453..1748
                     /locus_tag="VF089_11750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX6174680.1"
                     /translation="MSNVAIIGAQWGDEGKGKVVDLFTRDADIIVRFQGGNNAGHTLV
                     VDGKKTILHLIPSGALHRNKLCVIGNGVVVDPQILLEEIDALKAQGHLLDDSLLRISE
                     EGHLIMPYHKAIDLARERLRGKGKIGTTGRGIGPAYEDKVARVGIRFVDLLEEDTFRE
                     KLRHNIEEKNIYLKAILKEKTLDFDAIHDTYAGYRERLRPLITNTSLLLDRQIRAGKK
                     VMFEGAQGTLLDVDHGTYPYVTSSNTSTGGACTGAGVGPQHIHHVIGISKAYTTRVGS
                     GPFVTEQFGPDGETLKREGVEFGATTGRPRRCGWFDAVGVRHAVRVNGITGIALTKLD
                     VLTGFKKVPICTAYRFDGRLYDEFPASSKVLDKAEPVLEEMAGWNEALTAVRKFSELP
                     AAAQKYVQRIEKLLETEIILVSVGPGREQTILLKNPFDS"
     gene            1897..2202
                     /locus_tag="VF089_11755"
     CDS             1897..2202
                     /locus_tag="VF089_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX6174681.1"
                     /translation="MNGETSREKLISDLKTLVSDAEELLRATTSQAGEKVAAARQKIE
                     QSLIEGKKALADAEQALVETSKEYADIADDYIRENPWNAVAIAAGVGLLVGLTIRRS"
     gene            complement(2333..4048)
                     /gene="ggt"
                     /locus_tag="VF089_11760"
     CDS             complement(2333..4048)
                     /gene="ggt"
                     /locus_tag="VF089_11760"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6174682.1"
                     /translation="MQKQRQRTKDDEFMRCLFSLRFVCSILLINIFLAPTLVLAQRAN
                     GLVVSDSPLATKAGMEMLGRGGNAIDAAIATAYALAVVDQASSGIGGGGFMVIYETKD
                     RRAHALDFRETAPEGTRKELYTKDGRPAPSLSLTGGLAVAVPGEVAGLAQAHRRFGSM
                     PMQVLMAPAIRLAANGFPLDATLRYAIDRQQGAIRKFSDLGRIYMPTDEVPEEGELIR
                     QPELGETLKAIASQGPDAFYQGWIADAIVETVKREGGVLTNEDLKSYRPVWREPLIGN
                     YRQRTVISMPPPSSGGVAILQMLKVLESYQLSALPHNSATYLHLITETMKHAFADRAQ
                     YLGDPDFVKAPVSVLTSKDYARWIRGRISAAKTHPTRYYGLVNFKPELGGTTHFSVMD
                     RFGNAVACTVSINTRFGSKLLVPSTGIVLNNEMDDFAIHSSGNVYGLVGNEANSLQPR
                     KRPLSSMSPTIILQGERPEVVVGGAGGPRIISATLQTILNVLDYRMPVAKAVEAPRVH
                     HQWLPDQLNVEREIPIETKRSLERRGHAVRERGVLGVVQAITAGQDGIKGAADPRKDE
                     RARSE"
     gene            4138..7473
                     /locus_tag="VF089_11765"
     CDS             4138..7473
                     /locus_tag="VF089_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX6174683.1"
                     /translation="MPYRTPYSILALRHSNNPKIMQAILGPFHPHLENSLVEEILKYK
                     SNDPLCPLLILVPSDALRRHLKVLLAKDRNLSFVNLQLLTFYQLSAKLFAEAKGVNPP
                     VLRDDLFLEEALRQIIRARHPGTAAFSGIDERAGGCAALWQSLRDLRDGLVQPDVALE
                     ALRENQFDPRTKERTANLLVMLQTLVRFCREHGILDHSTLDRFAIAQAPASGFLRQFA
                     RIFYYGFYDLIQTQVDLVQAVAQAFPTTVFFPLLKTQPAHEGWQFAERFYSGYIQGRA
                     ASEEAKNLLLDCDHPAELPQSFAVFDHDPDRQYQTLPANWHCRIINTFGIDDEISAVA
                     KEILLLSEQDGIAFDQVGVVARTLDGYGAKIKELFAQHQIPINGSIEEPLVQYPLTKA
                     AILLMNLAAKDYLRSDVIDLLSSPYFRSNGSTQKPRELRPDLWDLATRELAICKGMKE
                     WQRIDRYAFEGMKLLQISDDEEWRFIEIPAAQLRALATILQSLDKVLGQLPAHASWTD
                     YADSWKQLFGAYLGIQPEPSRAFNATEILISNQIIETLDQISGLDAIGPNVSLHEFSK
                     TFQHWLERSTIVETDKKLAGVAVSSASAARGLKFRALFIVGMNEGVFPRTIREDAFLR
                     DRDREVLERDLGYKVSQKLAAFDEEKLVFTLLVNSVTDRLYCSYQRADESGRVLAPSW
                     YLAELKRALSGAQQGQFVEQTISRCTLDKVSCRPFVGEDSLLPVELAIRLNLSGTDSR
                     SLVEAAHLSSNLYEQGLKTIVALDLSTDPLDVFDGIIQTPARHWRRFSKRGLSPTALE
                     LYGRCPFQYFAGQVLGLQSLETPETAMGPGAAELGELGHLILKLTYQELIKSGYFANN
                     GSRVAVDAIISAAAQKAFTEYEAENPIGYPLMWETSRETLTQLIRDVVRRDLEELACS
                     GYVPVDLEIGIDDRLPPDWPDPLNTLPIHGRMDRIDIDPRANRMRVIDYKFKFGAKPS
                     PEDNDLSRSALRGQRLQPPLYSLLAQAKRRRDGLPDGEIQTEASFYYIASRWSEGPLL
                     IKSFNSGELSGRIGEEIRNSIAQLAKGIRSGHFFMQPGEHCPHCEVAEICRKNHPPSL
                     WRTENDPITAPHRQLRQKDLKKC"
     gene            7467..10910
                     /locus_tag="VF089_11770"
     CDS             7467..10910
                     /locus_tag="VF089_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6174684.1"
                     /translation="MLTANAMATDLADALDRRTAVETFDRNLVVTAGAGTGKTTLLVD
                     RIVHLLLRSPDPLAITDIVALTFTNKAANEMKQRLRDRLQAYLEVDLRTEPVEEEQKR
                     LQPQIESLMDTYRLSRDELDNRVQEALRNLERAEIGTIHSFAATLLRLFPLEAGVDPQ
                     FHEDDGKQFRRLFDQQWHLWLDQELALESPRAGEWRKALKQLTLEQIKDLARSLCSES
                     VQLEQPRTKQPENAVLRAWLRSLLDSAARLSERHPEDRNNEKLVRAARSMIQAFIDGG
                     DSTGTALAAEKACLLEHAINRETKGWTKADLKQAHSLVSAAKGLCRVDAALTHLMWKL
                     LAPYAARFREIFVHEGFISFDGLLIRARNLVRDHLRVREELKRQYRTFLIDEFQDTDP
                     IQYEILLYLAEQPGTPATNWRKVKLTPGKVFVVGDPKQSIYAFRRADIEAYLEVVDRI
                     IKAQNGIECHLLTNFRSDRAIIDVVNGVFSSLMQPQSGMQPPYIGIKPAPHRDSPALI
                     DVGKPLPRTLVRKIIGPDGDVSAETARRLESESLADWLQDQVLDNASFVNTRGERVLA
                     QPKDVAILFRKLTDIHDYLEPLRRRGIRYVVEGERHFYAAKEIIDAVNLLRAIENPND
                     RLALVGVLRSPIGGLTDQNIYDLHCDNLLDYRLTEQLCGKNFPPPLTELYCALAKLHA
                     DCRRLPVGAAVACIFDSLPLRILVACYFHGEQAVANIDKLRRQAELLGREDSTMTFKA
                     AIRQLQQRVLDVEEEGESVLAEEDVDAVRIMSVHKSKGLEFPIVVLAGCHAGINGQQR
                     RPAEALFDWSSGLTGIRVGSFTDLAGVYIAEKNRLRAEEETKRILYVAMTRAREHLII
                     SSGPSARKFSGNFAGMLDEAAQDQIGLAERSTTVEIGPGRLAVEPVQANLTAPGRVSG
                     NKKPRQKKQDWQAYMEVWRRREQNHRAALQIPTFVTPTSLKVHEQEITEAAVTTQTFA
                     RQRTPAMLLGELAHRFLEDWNFCGDRHLLRDRLGAFLSASLPTENSQHASSIDEELKM
                     IFERFVKSDIYAELRKARILGREVPLLMPWNGQIMEGVIDLIYEHNGLLYLADYKTDR
                     IEANDLHLRAERYRQQAEIYSEAARRSLKREVTAFKLIFLRLGETVQIEFNPDKELWL
                     F"
     gene            10911..11549
                     /locus_tag="VF089_11775"
     CDS             10911..11549
                     /locus_tag="VF089_11775"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX6174685.1"
                     /translation="MSSMKIFFFDIETIPTDQSVLDNNLLAAQMRLDEPEIIKKLSLS
                     AATARILCLAYAIEPQTDSPVEILNGTESEIISSFWQLAADCHLFVGHNILDFDLRFI
                     YQRSIINRIRPSREIPLARFRNDPVYDTMHEWSKWGREHVSLDLLARALGIPSPKESL
                     DGSKVYPYFCAGKLPDICDYCKRDVETVRQVYRRLTFTESSQPMIPQVDVPE"
     gene            complement(11777..12232)
                     /gene="msrB"
                     /locus_tag="VF089_11780"
     CDS             complement(11777..12232)
                     /gene="msrB"
                     /locus_tag="VF089_11780"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6174686.1"
                     /translation="MQKNWRALLAKDFKAPAPSEPLKLSNDEWRRRLDAAQFQVLREE
                     GTEAPGTSVLNGEKRPGVFICAACTLPLFTSAMKYESGTGWPSFFTTIPGVFGTRTDY
                     KLILPRTEYHCIRCGGHHGHLFSDGPRPTGQRWCNNGVALKFIPQSGSV"
BASE COUNT         2892 a         3547 c         3404 g         2716 t
ORIGIN      
        1 aattacgtca acgcgccgat actggccaaa gagcgcggta tccaagtggt tgaatccaag
       61 agcagcaagg ccggcgattt ttccagttcc atcaccgtca agatcgccac ggctgaccat
      121 gaagtcgaag tcgagggagc cattttcggc gacaaacacc cgcgcattgt ccgtgtcgat
      181 aatttttatc tggaagcggc gcccgaagga tatattctta ttttgcgcaa caaggacgtg
      241 cccggcgtgg tcggtaacgt cggcaccata ctcggtaaaa acggcatcaa catcgccggg
      301 atggaactcg gacgcggcgc aaaaggcggc aatgccattt cattcattca cgtcgatgat
      361 ctgattccca aagagacaat gaaagagctg cggagtctgc cgcaaaccgt ttcagctgaa
      421 ttagtcaaac tatgagccgg gtgcctgatt agatgtccaa cgtagcaatc atcggagccc
      481 agtggggcga cgaaggtaaa ggcaaagttg tcgatctttt tacccgcgac gccgatatca
      541 tcgtccgctt ccaaggaggc aacaacgccg gccacaccct ggtggtcgat ggcaaaaaga
      601 ccattctgca tctgattccg tccggggcgc tgcatcgaaa taaattatgc gtcatcggca
      661 acggcgtcgt ggtcgatccg cagattttgc ttgaggagat agacgctctc aaggcccaag
      721 ggcatttgct cgatgattca ctgctgcgta tcagcgaaga aggtcatctc atcatgccct
      781 accacaaggc catcgatctg gcgcgggaac ggctcagagg caagggaaaa atcggcacca
      841 ccggccgggg catcggcccg gcgtacgagg acaaagtagc gcgggtaggt atccgctttg
      901 tcgatctgct cgaagaggac acctttcgag agaaactccg ccataacatt gaagagaaaa
      961 atatttatct caaggcgatt ctcaaagaga agactctcga tttcgatgcc atccacgaca
     1021 cttacgccgg ctaccgtgaa cggctgaggc cactcattac caacaccagt ttattgcttg
     1081 atcgtcaaat ccgcgcgggc aaaaaagtga tgttcgaagg cgcccaggga acgttgctcg
     1141 atgtcgacca cggcacctac ccttacgtaa cctcttccaa cacgtcgacc ggcggcgcat
     1201 gcaccggcgc tggagtcggc ccccaacaca ttcatcatgt gatcggcatc tcgaaggcct
     1261 atacgacgcg ggtcggcagc ggcccttttg tcactgaaca gtttggaccc gacggtgaaa
     1321 ccttgaagcg ggaaggcgtt gaattcggcg caaccacagg ccgtccgcgg cgctgcggtt
     1381 ggtttgatgc agtcggcgtg cgtcacgcgg tgcgcgtaaa cggcataacc gggatcgctc
     1441 tgaccaagct tgatgtcctc accgggttca agaaagttcc gatctgcacc gcctataggt
     1501 tcgatggccg gctttacgat gaatttcctg ccagttcgaa agtgctggac aaggcggaac
     1561 cggttctgga ggaaatggca ggatggaacg aagcgttaac cgcagtgagg aagttctccg
     1621 agctgccggc ggccgcgcaa aaatacgttc agcgtatcga gaagcttttg gagactgaaa
     1681 tcattttggt ttccgtcgga cccggccggg aacaaaccat cctgctgaaa aaccctttcg
     1741 acagttaaca agagtcgttt gctaaacagt cctatgctat ggcttggaat catccaaatc
     1801 cacctggctg aactttcatg agaatcgagc ttgaatacct gctggcccca tgtttaacat
     1861 gggcttacga tcgaagcagg aacccgagga ggacagatga acggcgaaac ttcaagagaa
     1921 aaactcatca gcgatctcaa gactctcgtc agtgacgcgg aagaactgct acgagccacc
     1981 accagccagg ccggcgagaa agtcgcggca gcgcgacaga aaatcgagca gagcctgatc
     2041 gagggtaaga aggcgctggc cgacgccgaa caagctctag ttgaaacttc caaagaatac
     2101 gccgacattg ctgacgacta cattcgcgaa aatccgtgga acgcggtcgc tattgccgcc
     2161 ggcgtaggtc tactagttgg cctgacgatc cgccgtagct aaactgtcga gaagttttta
     2221 ccggaagcgg cacggcgttt cgtaaccttc tggcactcga gttcagattg ccaagcgcaa
     2281 ttgcaccatc ttccctaccc gacattgcga cgttcgtgcc gatgtgagga actcactcgg
     2341 atcgtgccct ttcgtctttt cgaggatcag ccgctccttt gatcccatcc tgcccggcag
     2401 tgatcgcctg cacaacgccg agcacgcctc gctcgcgcac tgcatgccct cgacgctcga
     2461 gtgatctctt ggtttcgatg ggaatttcgc gctcgacgtt taactgatcg ggcaaccact
     2521 gatgatgcac gcgaggggct tcaaccgcct tagccacggg catccgatag tccagcacat
     2581 taagaatcgt ctgaagcgtg gcgctgatga tgcgcggtcc gcccgcgccg cccaccacaa
     2641 cctctggccg ttcgccttgc agaattatgg ttggagacat gctgctcagc ggccgcttcc
     2701 tgggttgcag ggaattagcc tcattcccga ccagaccgta aacattgccg ctcgaatgta
     2761 tcgcgaagtc gtccatttcg ttgttcagca cgataccggt actgggcacg aggagtttgg
     2821 aaccaaagcg ggtgttgatc gaaacggtgc aggccacggc gttgccgaaa cggtccatca
     2881 cgctgaaatg cgtggttcca cccagctccg gtttgaaatt aaccaggcca taatatctgg
     2941 tcggatgagt tttcgccgca gagatgcgac cgcggatcca cctggcgtag tctttggaag
     3001 taagcactga aaccggcgcc ttgacaaaat ccggatcgcc aagatactga gcgcgatcgg
     3061 cgaaggcatg tttcatggtt tcagtaatca ggtggagata cgttgccgag ttatgcggca
     3121 aagcgctcag ctgatagctt tcgagcactt tgagcatctg caaaatcgcc acacccccgg
     3181 aactgggcgg cggcatgctg atgaccgttc gctggcgata attgccgatg agaggttctc
     3241 gccacaccgg ccggtaactt ttcagatcct cgttggttaa aaccccgcct tcccttttga
     3301 cggtttcgac aatcgcgtcg gcaatccaac cttgatagaa agcgtcgggg ccttgagagg
     3361 caatcgcttt caaagtctca ccgagttccg gctggcgaat caattcgcct tcttccggca
     3421 cctcatcagt tggcatgtag attcgcccga gatcggagaa cttcctaatc gcgccctgct
     3481 gccgatcgat ggcgtagcgc aaagtcgcgt ccagcggaaa tccgttggca gcgagcctga
     3541 tcgccggcgc catcagcacc tgcatcggca tgcttccgaa ccgcctgtgc gcctgcgcca
     3601 aaccggcaac ctcaccgggc accgcgacgg ccaacccgcc ggtaaggctg agcgagggcg
     3661 ccggcctgcc gtccttggta tagagttctt tgcgcgtccc ttccggcgcg gtttcgcgaa
     3721 aatccagcgc atgcgcgcgc cggtcctttg tttcgtaaat gaccataaag ccaccgccgc
     3781 cgattcccga tgaagcttga tccaccaccg ctaaagcgta tgcggtggcg atggcggcat
     3841 cgatcgcatt cccgccgcgt ccgagcattt ccattcccgc tttggtcgcc aacgggctat
     3901 ccgatacgac caagccgtta gcgcgttgcg ccagcactaa cgtcggggca agaaaaatgt
     3961 taattaggag aatagagcag acaaaacgta gactaaacag gcagcgcata aactcatcat
     4021 ctttagttct ttgcctttgt ttttgcaact tatttacctg cataagagag cgtcgataga
     4081 cattgcgctg ataggcttcg gcgactcacc gagagagcga agccgcgcag tttacgcttg
     4141 ccataccgta ccccttattc catattagct ctgcgccact cgaacaatcc aaagatcatg
     4201 caagccattc tcggcccctt ccatccgcat ctggaaaact cgctcgtcga agagattctc
     4261 aaatataaga gcaacgaccc gctctgtccc ctactcattt tagttccgtc cgatgctcta
     4321 cggcgccatc tcaaagttct gctagctaag gaccgaaact tatcctttgt aaatctgcaa
     4381 ctgctgacgt tttatcagct gtcggcgaag ctgtttgcag aagccaaggg cgtgaatccg
     4441 ccggtcttgc gcgatgatct ttttctcgaa gaggcattgc gccaaattat ccgcgcgcgc
     4501 cacccgggca cggcagcttt cagcggcatc gatgaacgcg ccggcggatg cgccgcgctc
     4561 tggcaaagcc tcagagatct acgcgatggc ttggtacaac cggatgttgc gttggaagca
     4621 ttgcgggaaa accaattcga tccgcgaacc aaggaaagga cagccaatct cttggtgatg
     4681 cttcaaacgc ttgttcgctt ctgccgagag cacggcatac tggatcattc caccttagat
     4741 agatttgcca tcgcgcaggc gcccgcatcg ggttttttac gccagttcgc gcggattttc
     4801 tattatggct tttacgatct catccagact caggtggatc tcgttcaggc ggtcgcgcaa
     4861 gcttttccga cgactgtgtt ctttcctctc ctgaagacac agccagcgca tgaaggttgg
     4921 caattcgccg agcgctttta ctcagggtac attcaggggc gggccgctag cgaggaagca
     4981 aagaatcttc tgttggattg cgatcaccct gctgagttgc cgcaaagttt cgcggttttc
     5041 gatcatgacc ctgatcgaca ataccaaact ttgccggcca attggcattg ccggatcatc
     5101 aatacctttg gcattgacga cgagatcagc gcggtggcca aggagatcct gctcctgagc
     5161 gagcaggacg gcatcgcgtt cgatcaggtc ggcgtggtcg cgcgaactct ggatggctat
     5221 ggcgcaaaga tcaaagagct ctttgctcaa catcaaattc caatcaatgg ttccatcgag
     5281 gaaccgctgg tgcagtatcc tttgactaag gctgccatcc tgctgatgaa tcttgcggcc
     5341 aaagattacc tgcgctcgga tgtgatcgac ctcttgtcct cgccctattt ccggtcaaat
     5401 ggctcgacac aaaagccacg cgagctgcgc cccgatcttt gggatctagc cacccgagag
     5461 ctggcgatct gcaaaggaat gaaggaatgg cagaggatcg atcgttatgc cttcgaaggc
     5521 atgaaactgt tgcagatatc cgatgacgag gagtggcgct ttatcgaaat tcctgcagcc
     5581 cagttgcgcg ccctagcgac tattctgcaa agcctagaca aagttttagg ccagctgccg
     5641 gcgcacgctt cctggactga ttacgccgat tcatggaagc aactgttcgg cgcctatcta
     5701 ggaatccagc ctgaaccttc gagagctttt aacgccaccg agatcttgat ctctaaccaa
     5761 atcatcgaga ctctggatca aatctccggc ctcgacgcta tcgggcccaa tgtttcgcta
     5821 cacgagttca gcaagacctt tcagcactgg ctcgagcgca gcaccatcgt cgagactgat
     5881 aaaaagctag caggcgtcgc cgtatcgagc gccagcgccg ctcgcggttt aaaatttcgt
     5941 gcgctgttca tcgtcggcat gaacgaaggc gtttttccgc gcaccattcg cgaagacgcg
     6001 tttctgcgcg accgcgatcg cgaggtgctg gagcgcgacc tcggctataa agtcagccag
     6061 aagctggccg cgttcgatga agagaagctg gtgtttacgc tacttgtcaa ttcggtcacc
     6121 gaccggctgt attgttcgta ccagcgcgct gacgaaagcg gcagagtgct ggcgccatca
     6181 tggtatttgg cggagctcaa gcgcgcgctc tcaggcgcgc agcagggaca attcgtagaa
     6241 cagacgatct cgcgctgcac cctagacaaa gtgagttgcc gtccgttcgt tggcgaagac
     6301 tcgctgctgc cggtggagct tgcgattcgt ctcaatcttt ccgggaccga ttcgcgatca
     6361 ttggttgaag cagcccatct ttcctccaat ttgtacgagc aaggcctcaa aaccatcgtc
     6421 gccctagatc tcagcactga cccgctcgat gttttcgacg ggatcattca aactccggca
     6481 agacactggc ggcgcttttc aaagcgtggc ctgtcgccca ccgcgttgga gctttacggg
     6541 cgctgcccgt ttcagtattt cgcggggcag gtgcttggac tgcaaagttt ggagacgccg
     6601 gaaactgcca tgggaccagg cgcggcggaa ctgggagaac tcggtcattt gattttaaaa
     6661 ctgacttacc aagaacttat caagagcggt tatttcgcaa acaatgggtc tcgtgtcgct
     6721 gtcgatgcga ttatcagcgc cgccgcacaa aaagccttca ccgagtacga ggctgagaac
     6781 ccgatcggtt atcctctgat gtgggaaacg tcgcgtgaga cgctgactca gctcatccga
     6841 gatgtcgtcc gccgcgatct agaggagctt gcatgttcgg gctatgtccc ggtggatctg
     6901 gagattggta tagacgatcg gttgccgccg gattggcccg atccattgaa cacgctgccc
     6961 atccacggcc gcatggatcg aatcgacatc gatcctaggg ctaatcgcat gcgggtcatc
     7021 gattacaagt ttaaattcgg cgccaaacct tcgcccgagg acaacgacct ctcccgatcc
     7081 gccctgcgcg gacaacgact tcagccaccg ctttattctt tgctggctca agcaaagcgg
     7141 cgccgcgacg gtttgcccga tggcgaaatt caaacggaag caagttttta ctacatcgcc
     7201 tcgcgctgga gcgagggtcc attgctcatc aaatccttca atagcggcga actatccggc
     7261 aggattggcg aggagattag aaacagcatc gctcaattag ccaagggcat tcgttcgggt
     7321 catttcttca tgcaaccggg agagcattgt ccacactgcg aggtcgcgga gatctgccgg
     7381 aaaaatcacc cgccgagtct ctggcgcacg gaaaacgacc cgatcaccgc tccgcaccgg
     7441 cagttgcgcc agaaagatct caaaaaatgc tgacggccaa cgcaatggct accgatctcg
     7501 ccgatgcgct cgatcgccgc acagccgttg agactttcga tcgcaatctg gtggtcaccg
     7561 ccggggccgg cacgggcaag acaactcttc tggtggaccg gatcgtccat ctgttgttgc
     7621 gcagcccgga cccgctcgcc atcacggaca tcgtcgcgct gacgtttacc aataaggccg
     7681 ccaatgagat gaagcagcgg ttacgcgatc ggcttcaagc atacttggag gtagatctgc
     7741 gaaccgaacc tgtggaggaa gagcagaaga ggctacaacc acagatcgaa agcttgatgg
     7801 atacttatag gctgtctagg gacgagctcg ataaccgcgt gcaagaggca ctgcgcaatc
     7861 tggagcgcgc cgagatcggc acgattcaca gctttgcagc gaccttgctc cgcctttttc
     7921 cactggaggc tggagtggat ccgcagtttc acgaagacga cggcaaacag ttccgtcgtc
     7981 ttttcgacca gcagtggcat ctctggctgg atcaggagct cgcgcttgag agccctcgcg
     8041 ccggcgagtg gcgcaaggcg ctcaaacagc tgacgcttga gcagattaaa gacctcgctc
     8101 gctctttatg ctcggaatct gttcagctcg aacagccccg aacgaagcaa ccggaaaatg
     8161 ccgtgttacg cgcatggctg cgctcactac tcgatagcgc cgcccgcttg agcgaacgcc
     8221 atcccgaaga tcgcaacaac gagaagctcg ttcgcgccgc ccgctcgatg attcaggcgt
     8281 ttatcgacgg cggtgattca accggaacgg ctttagccgc agagaaagcc tgtttattgg
     8341 agcacgccat caaccgggag actaaaggct ggaccaaagc tgatctcaaa caagcgcata
     8401 gcctggtcag cgccgccaaa ggcttgtgca gagtcgatgc ggctctgact catctgatgt
     8461 ggaaactcct cgcaccctac gccgctcgtt ttcgtgagat tttcgtccat gagggtttta
     8521 tctcattcga tggcctgctg attcgcgcgc gcaatttggt tcgcgatcat ctacgcgtgc
     8581 gcgaagaatt aaaacggcaa taccgaacgt ttctcatcga tgaattccaa gacaccgatc
     8641 cgattcagta cgagatcctg ctttacctgg ctgaacagcc gggcactcca gcgacgaatt
     8701 ggcgcaaggt gaaacttact cccgggaagg ttttcgtggt cggcgatccc aagcaatcga
     8761 tctacgcatt ccggcgcgcc gatatcgaag cctatctgga agtcgtcgac agaatcatca
     8821 aagcgcaaaa cggcatagaa tgtcatttgc ttacaaattt tcggagcgat agggcgatta
     8881 tcgatgtcgt caacggcgtg ttttcatcgc tgatgcagcc gcaatccggt atgcagccgc
     8941 cctacatcgg aattaaaccg gcaccccatc gcgattcgcc tgccctgatt gatgtcggta
     9001 agcctttgcc gagaacactg gtgcgaaaga ttattgggcc ggacggagac gtcagcgccg
     9061 agaccgctcg tcgtctcgaa agcgaaagcc tggccgattg gctgcaggat caggtgttgg
     9121 acaacgccag ctttgtgaat accagaggcg agcgggtttt ggcgcagcct aaagatgtgg
     9181 cgatcctctt ccgcaagctc accgacatcc atgactatct cgaaccgctg cggcgccgcg
     9241 gcattcgtta cgtcgtcgaa ggcgagcgcc atttctatgc tgccaaggaa attatcgatg
     9301 ctgtcaattt gctccgtgcc atcgaaaatc ccaatgatcg tcttgcattg gtcggcgtgc
     9361 tgcgttcccc catcggtggc ttgaccgatc aaaacatcta cgatctacac tgcgacaatc
     9421 tgctcgacta ccggcttaca gaacagcttt gcggcaagaa ttttccaccg ccgctaacag
     9481 aactttactg cgcattagcc aaactgcacg cagactgccg caggctgccg gttggcgctg
     9541 ccgtagcctg tatctttgac tcgctgccgc tgaggatcct cgtcgcctgc tattttcatg
     9601 gcgagcaggc cgtggccaat atcgacaagc tgcgacgcca ggccgaactg ctcgggcgcg
     9661 aagatagcac catgacattc aaggcagcta ttcgccagct ccaacagcgc gtgctcgacg
     9721 tcgaagaaga aggggaaagt gtgctcgccg aagaagatgt cgacgccgtg cgcatcatga
     9781 gtgtccacaa gtccaaagga ctcgagtttc ccattgtggt tctagccggc tgtcatgccg
     9841 gcatcaacgg ccagcagagg cggccggcag aagcgctgtt cgactggtcc agcgggctaa
     9901 ccggaattcg tgtcggctcg ttcacagacc tcgccggggt ttacattgcg gaaaaaaatc
     9961 gcttgagagc cgaagaagaa accaagcgaa tactctatgt cgcaatgacc cgggctcgag
    10021 agcatttgat tatttcaagc ggccccagcg ccaggaaatt ttccggcaat tttgccggca
    10081 tgttggacga ggcggcacaa gatcaaatcg ggctcgccga acggtcaacc accgtcgaga
    10141 tcggccccgg cagactggca gtcgaaccgg tccaggcgaa ccttaccgcg ccggggcgag
    10201 tcagcggcaa caagaagccg cgccaaaaaa aacaggattg gcaggcttac atggaggtct
    10261 ggcgccggcg tgagcaaaat caccgagcag ctttacagat accgaccttt gtaacgccca
    10321 cgtcgctcaa ggtccacgag caagagatca ccgaagcggc cgtgacaact cagactttcg
    10381 cccggcagcg cacgccggca atgctcttgg gcgagctggc gcaccgattt ctcgaggact
    10441 ggaatttttg cggcgacaga cacttactgc gcgatcgact cggtgctttt ctcagcgcat
    10501 cgctgccaac cgaaaacagc caacacgcaa gcagcataga cgaagagctg aaaatgatct
    10561 tcgagcggtt cgttaagtcg gatatctatg ctgaactgcg caaggctcgg atcctcggcc
    10621 gtgaagtacc gctgttgatg ccctggaacg gccagatcat ggaaggggtc atcgacctca
    10681 tctatgagca taacggcctg ctttatctgg ccgattataa aacagaccgg atcgaagcga
    10741 acgatttgca tctccgcgcg gagcgctacc gccaacaagc cgagatctat tccgaagcgg
    10801 cgcggcggag cctgaaacga gaagtcacgg ctttcaagtt gatatttttg cgcttgggcg
    10861 agaccgtcca aatcgaattt aatccagata aggagctgtg gcttttttag atgtcatcca
    10921 tgaaaatttt cttctttgat atcgagacca tccccacgga tcagtcggtg ctcgataata
    10981 acctgctggc tgcgcaaatg cgtctcgatg aacccgagat tatcaaaaag ttgagtcttt
    11041 cggccgcgac agcgcgcatt ctatgcctgg cttacgcgat cgaaccgcaa acggattctc
    11101 ctgtcgagat actgaacggt accgagtctg aaatcatcag cagcttctgg caactggccg
    11161 cggactgtca cttgttcgtg ggccacaata ttcttgattt cgacctgcgg tttatctacc
    11221 agcgttcgat catcaatcgg atcagacctt cgcgcgagat ccccttggct cgcttccgga
    11281 acgatccggt ttacgacaca atgcatgaat ggagtaaatg ggggcgcgaa cacgtgagcc
    11341 tggaccttct agcgcgcgcg ctcggaattc cgtcgccgaa agaaagcttg gacggctcca
    11401 aggtgtatcc ctatttttgc gcgggaaaac tgccggacat ctgcgactac tgcaaacgcg
    11461 atgtggaaac cgtgcgccag gtatatcgcc gtttgacgtt tacggaaagc agtcaaccta
    11521 tgatcccgca ggtagatgtt cccgaataac ggtgttccgc agtaagctgt ccagcttgct
    11581 tacctgcccg cgtttgtgag ttcgaccagg ggaaactcct tacctcgtag attgctgaaa
    11641 aactctggtt ccaggctgct caaaaagatc cataggcgag gcgcgcgaaa aatcgacgag
    11701 ctaaggcgta cttacagtac gttgcagcga ggcggtttga gcgcaacgaa gcatatgggt
    11761 ctttttcagc agcctgttag acgctgccgc tctgtggaat gaactttaac gctacgccat
    11821 tattgcacca gcgttggccg gtaggcctgg ggccatcgct gaatagatga ccatgatgtc
    11881 cgccacagcg gatgcagtga tactcggtgc gcggcaggat cagcttataa tcggtgcggg
    11941 tgccgaatac gcctggaatt gtcgtgaaaa aactcggcca gccggtgccg ctttcgtact
    12001 tcatcgccga ggtaaagagc ggcagagtgc aagcggcgca aatgaaaaca cctggacgtt
    12061 tttcaccgtt cagcacgctg gtgccgggag cttcggtccc ctcttcgcgc aagacctgaa
    12121 attgcgcggc atccaaacgc ctgcgccact cgtcattgga gagcttgagc ggctcggatg
    12181 gggcaggagc tttgaaatcc ttggcaagca acgcgcgcca attcttttgc atgttctcga
    12241 tatctttcat agacaatgcc tgtccggcgc gtccagctcc tccaagcaga tgccaaatac
    12301 ctgcagccag cgcagcttgt aacagttttc gtcttgtcag catggcgagt ccctttccca
    12361 ttttccatat tccccttaaa cgaatctcaa atcgatcata acacgaaccg cgcgcgtaag
    12421 tatggcgccg gccatttccc aggacccgcg gcatgttaaa ctatccatga tgaggttttc
    12481 cgaatttgcc gcgctttgtt acgagctgga gcagacctcg agccggttgg caaaagtcgt
    12541 tttggctgcg gaatatctt
//
LOCUS       DASUTG010000513         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1859685, whole genome shotgun sequence.
ACCESSION   DASUTG010000513 DASUTG010000000
VERSION     DASUTG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859685"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF089_11785"
     CDS             <1..213
                     /locus_tag="VF089_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174687.1"
                     /translation="DILYFTPAITSIMNVTDGSLTVIPELAYSPMTNLELRFRTPVLL
                     GKNGTEYGEKQNDYRIELRMRYHFQF"
     gene            472..936
                     /locus_tag="VF089_11790"
     CDS             472..936
                     /locus_tag="VF089_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX6174688.1"
                     /translation="MQKIHILILATVIGVVFVAGDSYAQKGPGTMWRGSGGWGQGSQY
                     NKMYDPKAVETITGEVTNVDRITPAKGMSGGLHMSVKTDKETISVHLGPSWYLENQDV
                     KIEAKDKVEVKGARTTFAGKPAIIAAEVKKGDEVLKLRDDNGFPVWSGWRRR"
     gene            1033..1437
                     /locus_tag="VF089_11795"
     CDS             1033..1437
                     /locus_tag="VF089_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX6174689.1"
                     /translation="MDWLRRGEWPWWQAGLLLGLLNMAAFFTADYYLSVSTTFSRAAG
                     MVVGLVAPEHVAANAYWQKVKPIVDWQFMLVLGIPLGAYLAARLTQKSVEFTGSLPDM
                     WVSRFGTSNAQRWVVGILGGIFVGFGARLADG"
     gene            1557..1859
                     /locus_tag="VF089_11800"
     CDS             1557..1859
                     /locus_tag="VF089_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX6174690.1"
                     /translation="MGPLEITGPARLALGLATGILFGFILQKAQVTQYQKIVSFFRWS
                     DLTVQKVMFAAILVGMVGVYALHDLGLVNLHIKPALLGANILGGLIFGAGMLLLGF"
     gene            1920..2138
                     /locus_tag="VF089_11805"
     CDS             1920..2138
                     /locus_tag="VF089_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174691.1"
                     /translation="MGILIGAGIYSEVYPLIENNLLQLGDYGKLTFPTVLGIDPWFVI
                     VPFAIIVIGVLLGMDRIDKQRSKATQGN"
     gene            2178..>2528
                     /locus_tag="VF089_11810"
     CDS             2178..>2528
                     /locus_tag="VF089_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174692.1"
                     /translation="MEHKKIIVTMITGAVVISANPLWAATATQDRTKEKIQTQTKDQE
                     QIYGSGLMTDKERSEYRERMRSAKTEQEREKLRNEHHNRMLARAKERGVKIPDEPPVK
                     GGGMGPGGGMGTGAG"
BASE COUNT          577 a          610 c          744 g          597 t
ORIGIN      
        1 gatattctct atttcacgcc cgccatcaca tccatcatga atgtcaccga tggaagcctc
       61 accgtcattc ccgagctcgc ctacagcccg atgaccaatc tcgagttgcg cttccggacg
      121 cccgtcctgc tcggcaaaaa cggcacggaa tatggagaga agcaaaacga ctatcgaatc
      181 gaactgcgaa tgcgctacca cttccagttt tgaatctgtc tttcccggtg gcggtcttat
      241 ttttctctca tcacaacaat cacgcgggcc aacggatggg gtttttttga ttgcacgcgg
      301 tccgttggtt ttgagtacag ctagcgccga cgattattac tcgcgcgaat tcatccatcg
      361 tattttcgca attgtgaaag aggcggtgtc gccggctgct atttaaatac gacttctgta
      421 tggcataggc gttgctggtt ctctcccaca aacaacggag gtgaaaagga aatgcaaaaa
      481 atacacatac ttattttggc tacggtaatc ggcgtagtct tcgtggcggg ggattcctat
      541 gctcagaaag gacccggaac gatgtggcgc ggcagcggcg gatggggaca aggaagccag
      601 tacaacaaga tgtacgatcc taaggcggtg gaaacgatta ccggggaagt aaccaacgtg
      661 gatcgtataa cgccggccaa agggatgagc ggtggacttc acatgagtgt gaagaccgac
      721 aaggaaacga tctccgttca tctgggtccg agctggtatc ttgaaaacca ggacgtcaag
      781 atcgaagcga aagacaaagt cgaagtgaag ggcgcgagga ccacttttgc cggcaaaccg
      841 gcgatcatcg cggcggaagt gaagaaaggc gatgaagttt tgaaattgcg tgacgacaac
      901 ggcttccctg tgtggagcgg ttggcgcagg cgttagtttc gaacgaaccc ttgtttgcac
      961 ggttttagtc ggcggctgtt tggcacggaa agctctcccg ccgctggtcg ctgacgttgg
     1021 aaggaattag ctatggattg gctacgtaga ggagagtggc cctggtggca ggcgggttta
     1081 ttactcggcc tgctaaacat ggccgcattt ttcacggcgg actattacct gtcggtttcg
     1141 acgactttct cgcgcgcggc gggaatggtg gttggactcg tcgcaccgga gcatgtggcg
     1201 gcaaacgctt actggcagaa ggttaagccg atagtcgatt ggcaattcat gcttgttctg
     1261 ggaattccac tcggcgccta tctggccgcg cgactgactc aaaaatcggt cgagttcact
     1321 ggatcgcttc ccgatatgtg ggtcagccga ttcggtacga gcaatgcgca gcgttgggtt
     1381 gtcggcatat tgggcggcat tttcgtcggt ttcggtgcgc ggctggctga tggctgaacc
     1441 agcggccacg ctctcagtgg agggcttcaa atggccgtga gcagttggat ctttcttatc
     1501 gcgatgatga tctcaggcac gatcatggcg cggctaattt tccggagggt gtagctatgg
     1561 gtccattaga aattaccggt ccagcgcgtc tcgcgctcgg actcgcaacc ggaattctct
     1621 tcggtttcat tttacaaaag gcacaggtca cccagtatca gaagatcgtg tctttctttc
     1681 gttggagcga tctgactgtc cagaaagtca tgtttgcggc gatactcgtc ggtatggtcg
     1741 gagtatatgc gctgcatgat cttggtttgg tcaatcttca catcaagccg gccctgcttg
     1801 gcgccaatat tcttggcggc ctgatttttg gcgccggcat gttgcttttg ggtttttgac
     1861 ccggcacctc tgctgcggcc ttcggcgaag gccgtttaga tggcttgttt cgcggtgttt
     1921 tgggcatttt gatcggcgcg ggtatttact cggaagttta tcctttgatt gaaaataacc
     1981 ttctccagct cggcgattac ggcaaactga cctttccgac cgtcctcggt atcgatccct
     2041 ggtttgtgat cgtgccgttc gcgatcatcg tcattggtgt tctcttgggg atggaccgaa
     2101 tcgacaagca aagaagtaaa gcgacgcaag gaaactgaat ccgcaatatt aacatgatca
     2161 atttgaggag gagaaaaatg gagcacaaga aaataatcgt gaccatgata accggcgcgg
     2221 tcgttatttc ggcgaatccg ctatgggcgg cgaccgctac ccaagatcgg acgaaagaga
     2281 aaatccagac gcagaccaaa gatcaggaac agatctatgg cagcgggctt atgaccgata
     2341 aagagcgcag cgaatatcgc gagcgaatgc gcagcgcaaa aaccgaacag gagcgtgaga
     2401 agcttcgcaa cgagcaccac aatcgcatgc ttgcgcgcgc caaggagcgt ggcgtgaaga
     2461 ttcccgacga gccgccggtg aaaggcggcg gtatgggtcc tggtggcggg atggggacgg
     2521 gcgctgga
//
LOCUS       DASUTG010000514         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_969810, whole genome shotgun sequence.
ACCESSION   DASUTG010000514 DASUTG010000000
VERSION     DASUTG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969810"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VF089_11815"
     CDS             complement(<1..1340)
                     /locus_tag="VF089_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6174693.1"
                     /translation="MVKQSLKFVGQSVPRVDGIEKVTGQAKFLGDLAVPGMLHGKVVR
                     SSYPHARIVSIDASKAEALEGVAAVLTNADISDLNAIYSGRPVIAVNKVRYVGEPVAA
                     VAAIDQATAEQAAALIEVHCEELPAVVGIDIARAPGAALIHEGRNDNICSHESVVRGD
                     VEEAFAQSHLVLEDQFTFPMVYHYAMEPHAVIAHWNEDGVTVWSSAQHPFQVRSDIAK
                     VFRLPTSRVRMVISYLGGGYGSKSYTKFEPLVVALARKAKAPVRVCNSVGESMITVRR
                     HAAQVRVKTGFKNDGTITAREAEIYLDTGAYDDNGPAVTVRAATRVIGPYRIPNILTH
                     AYSVYTNSGSAGSFRAIGAPQVIFAGESQIDIAAARLGIDPAELRQKNLLKRGEELRP
                     GLKGIDADLNYGLKKLVGASQWKSITKKMAAPVGLACAVTNAGATPVSVAMARL"
     gene            1512..2207
                     /gene="sfsA"
                     /locus_tag="VF089_11820"
     CDS             1512..2207
                     /gene="sfsA"
                     /locus_tag="VF089_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX6174694.1"
                     /translation="MRFPDRLIRGILIRRYKRFLADIRLADGQVVIAHCTNTGSMTGC
                     AEPGSTVYISPSTKEGRRLLYTWEIVKVATTWVGINTLHPNRLVAESIEKGVISELQG
                     YGTIHREVATRQGTRLDLRLDGVNGSCFVEVKNVTLAIDGAAAFPDAVSERGTKHLKE
                     LIWLRRKGHRAAVVFVIQRGDCNYFRPADEIDSEYGRWLRRAVKAGVEALPYRARVTA
                     RELVLTERVEARF"
     gene            complement(2308..>2908)
                     /locus_tag="VF089_11825"
     CDS             complement(2308..>2908)
                     /locus_tag="VF089_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174695.1"
                     /translation="ADATDDPEVNNMVNEVLERHGKIDVLVNSAGLRGPLVPVQDITE
                     EEWDLVLSVNLKAVFLCCRAVLKVMIKQKSGSIVSISGTAGKEGMALRGSLCAAKWGL
                     LGLTQTIAKEAGPYGIRANIICPGGMDEPDLRVMYAERAKGLGMEFAQLEKSILELTP
                     LRKYAKHEEVAKAALFLASSDSSHTTGESLNVSGGRLMS"
BASE COUNT          661 a          834 c          762 g          651 t
ORIGIN      
        1 tgcaggcgtg ccatcgcaac cgataccggc gtcgctcccg cattggtcac ggcgcaggct
       61 aatccaactg gcgcagccat cttcttggtt atggatttcc attggctcgc gccgacgagc
      121 ttcttaaggc cgtaattcaa atcggcatcg atgcctttaa ggcctggacg gagttcctca
      181 cctcttttca aaagattttt ctggcgcaat tctgccgggt ctatgcctaa acgggccgcc
      241 gcaatgtcga tttgcgattc gccggcaaaa atgacttgcg gtgcaccgat cgcacgaaac
      301 gaccccgcgg aaccggagtt ggtataaact gaatacgcgt gcgtcagaat attaggaatt
      361 cgataaggac caatgacgcg ggttgccgcg cggacggtca cggccgggcc attgtcgtca
      421 taggcgccgg tgtcgagata gatttcagcc tcccgcgcag tgatcgtacc atcgttctta
      481 aaaccggttt tcaccctaac ctgggccgcg tggcgccgca ctgtgatcat ggattcaccg
      541 accgagttac acacccggac aggcgccttc gctttgcgcg ccaaggcgac gaccagcggc
      601 tcgaacttgg tgtaggactt actgccatag cctcctccca aataggagat caccatccgt
      661 actctcgagg tcggcagacg aaatactttg gcaatatcgc tccgtacctg gaagggatgc
      721 tgcgccgaag accacacagt aacgccatcc tcattccaat gggcaatgac ggcgtgcggc
      781 tccatcgcat agtgataaac cattggaaac gtaaactggt cttcgaggac caaatgagat
      841 tgtgcgaatg cctcttccac atcgccgcgg acgacgctct cgtggctaca gatgttatcg
      901 tttcgccctt cgtgtattag agcagcgccc ggggcgcgcg cgatatcgat gccgacaacg
      961 gccggaagct cttcacaatg cacctcgatc agcgcggcag cctgctcagc cgtggcctga
     1021 tcgattgccg ccaccgctgc tactggttct ccgacgtatc gaaccttatt caccgcaatc
     1081 acggggcggc cggagtaaat cgcattgagg tccgatatat cggcattggt cagtaccgcc
     1141 gcgacgcctt caagcgcttc agctttggag gcgtcgattg agacaatcct cgcgtgagga
     1201 taggaactgc gaaccacctt cccgtgcagc atcccgggca cagccaaatc gccgagaaat
     1261 ttcgcctggc ctgtaacttt ttcaatgcca tctactcgcg gcacactctg accgacaaac
     1321 ttgagcgatt gtttgaccat tgctctccat ccacagctaa ttttcgttga acctaacaaa
     1381 gcgatgtttt ccgcgcaagg aagctgcagc gcgtagcgcg aagtcgacaa cagcatcgat
     1441 tgatgatgtg gctcggaatc agcaaacctc cgatgttagg aatcgattga cacatcggct
     1501 ataactttgc catgcgtttt cctgatcgac tgatccgcgg cattctcatt cgccgctata
     1561 aacgattcct cgccgacata cgcctggctg acggtcaagt cgtaatagcg cattgtacca
     1621 ataccggcag catgacaggc tgcgccgaac ccggtagcac cgtttatatt tcaccaagca
     1681 caaaggaagg ccgccgtttg ctgtatactt gggaaatcgt caaggtcgca acaacttggg
     1741 ttggcatcaa taccctgcat ccgaaccgat tggtcgccga atctatagaa aagggcgtca
     1801 tcagcgaact gcagggatat gggacaatcc atcgcgaggt ggctacgcgc cagggtacac
     1861 gattagatct gcggctcgat ggcgttaacg gctcttgttt cgtcgaggtc aaaaatgtca
     1921 cactcgcgat cgatggtgcg gcggcatttc ccgatgcagt tagcgagcgc gggacaaaac
     1981 atttaaaaga attgatctgg cttcgcagaa aagggcatcg agccgcggta gttttcgtga
     2041 tccaacgcgg cgactgcaat tattttcgac cggcggatga aatcgactcc gagtatggtc
     2101 gctggttgcg ccgagccgtc aaagcgggag tcgaagcgct cccttaccga gcacgggtta
     2161 cggccaggga attggtcctg accgagcggg ttgaagcgag attctgatca gcttctgcat
     2221 gatacggccc cgctaccgct ccaaacgttg ttgccacaag cgtgtcagct ctgctaacac
     2281 gaatgcccgt aaagaatggt tcaaatgcta gctcatcaag cgcccgccgg aaacgttgag
     2341 agattctccg gtggtatgcg aagaatcgct cgaagccaga aacaaagcgg cttttgcgac
     2401 ttcctcatgt ttggcatact tgcgcagagg cgtcagctct aagatgcttt tctccaactg
     2461 cgcgaattcc attcccaatc ccttggcccg ttctgcatac atgacccgca ggtcgggctc
     2521 atccatacca cccggacaaa tgatattggc tctgatgccg tagggtccgg cctctttggc
     2581 gatggtctga gtcaacccca ataatcccca cttggcggcg caaagagaac cgcgcagggc
     2641 cataccttct tttccggcgg tgccggagat gctgacgatg ctgccgcttt tctgtttgat
     2701 catcactttt aacactgccc tgcagcacag aaacacagcc ttcagattaa ctgaaagaac
     2761 aagatcccat tcctcctcag tgatgtcttg aaccgggacg agcggcccgc gtaatcccgc
     2821 gctgttgacg aggacatcga ttttgccatg acgctcgaga acttcgttca ccatgttgtt
     2881 gacctcagga tcatcagtgg catcggca
//
LOCUS       DASUTG010000515         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_80312, whole genome shotgun sequence.
ACCESSION   DASUTG010000515 DASUTG010000000
VERSION     DASUTG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80312"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..712
                     /locus_tag="VF089_11830"
     CDS             254..712
                     /locus_tag="VF089_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174696.1"
                     /translation="MATAKDTAQPLANLLNIAPQVIPTADPPHEYFQATANAVRRLKD
                     ETVLVVGHITIPNIIAEFGGPQLPTICEGVFSDLFLLIPALGRKASQDCDLALRRRFR
                     RAAEICLDCDRIQTTYDEITTDFARHVNSSQRFDIVRLLLLELKEHGNAV"
     gene            810..1559
                     /locus_tag="VF089_11835"
     CDS             810..1559
                     /locus_tag="VF089_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174697.1"
                     /translation="MLPPILAIASVLAVWWGLYLVFPRLLPGPVSTFNEAIRLLSDGT
                     FFFHMLQSLRRVFVGAAVAMIFSIAIGIYMGTVLMGERFFQPLVVLGLTIPGLMWALI
                     AVMLFGINEIASYFAVAVTTFPMLVINIWAGVKSLDKELIDMSHVFRFTPWMKISQVI
                     IPQLVPNIFAATRYGLGLAWKVVVVVEMFGTSNGVGYQVMKSYQVFNMEGVIAWTLTF
                     VAAMIIIEYGIINLAERRLTAWRPKVDVWRR"
     gene            1560..2324
                     /locus_tag="VF089_11840"
     CDS             1560..2324
                     /locus_tag="VF089_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6174698.1"
                     /translation="MARLIVRDVEKWFRREDGQPLKILDRLSFEALEGCVTVVVGPSG
                     CGKSTLLNAIVGLERLDRGELAFATEDVHLQRRPLIGYVFQSPRLLPWKTVGDNLRLA
                     LQGAGVARGEWQERVERYLDLVGLGEYAAQFPLYLSGGQRQRVGLARALAIESDLVLM
                     DEPFASVDELTARQLRQTTRRLCEQLHRTVLFVTHNLPEAAYMSDYVVTLTTRPARLA
                     EYVTNPLPAPRNWTPEIYQFAAELEAKVTFKEESEL"
     gene            2321..3289
                     /locus_tag="VF089_11845"
     CDS             2321..3289
                     /locus_tag="VF089_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174699.1"
                     /translation="MNLSIVISSIAIMITLSSFCRAPVQAQSLQTVNIATAAAGQSGY
                     IAALIDAKGIAAKHGLKINNLMMDFTEAANALKLGRAIASAMQPSTAVNLLKSGTDIR
                     LIAPQIWSGNSWLVKKDSPYKSLADLKGKKIGNFARVTGAYFFSAVIAKEKKLDIEKD
                     FQSIPAETGALIALLERGEVEAINMFEPHTTRLVLSDKYRVLLDFDVELEKLFGAPPL
                     KSSVGMLRETVEKQPALVKAIRAAYVEAVKMIKAGQDKDFFQAKAKEFFNLSTPEQVA
                     AGMKQNRQNFADLWGESFFQSQNKILQSGISLGLLPKVNVTELWIK"
     gene            complement(3336..4004)
                     /locus_tag="VF089_11850"
     CDS             complement(3336..4004)
                     /locus_tag="VF089_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174700.1"
                     /translation="MKTASSLINPGESLHDEAEAPWQMDGLDQTPASSYARDLRVVGQ
                     LLERRQITRADLVCVADAYIVRGIQKSSGDSRFLSYLPRRFMPRLRAILHGDFRPSLR
                     SRIINLRYDLEEIVKADYLARCKRSSISPLPDPYSLSHILRSAGAYLDGRKNSSLIGI
                     SLQDRWVTIRFETAAGYPEETKQNLQYFYDYWVKMYLRRASHGQMQTAGSNLHHNSFM
                     ANRR"
     gene            complement(4339..>5089)
                     /locus_tag="VF089_11855"
     CDS             complement(4339..>5089)
                     /locus_tag="VF089_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6174701.1"
                     /translation="GATKARDLPIPGRELDGIHFAMDFLHANTKSLLDSNHKDNRYIS
                     AKDKHVIVVGGGDTGTDCVGTSMRHGCKSLTQLEILPRPPDTRQPDNPWPEWPKVYKL
                     DYGQEEAKARFGADPRIYLTTGEKFVGDSDGRVKELHVYDVEWARNEQGAFIPKRVPG
                     TDKILRADLVLLAMGFVGPEETVLAQLGVERDPRSNAKAEYGEFSTNVPGVFAAGDCR
                     RGQSLVVWAINEGRGAARECDRFLMGTTELA"
BASE COUNT         1152 a         1345 c         1373 g         1219 t
ORIGIN      
        1 tcatccttaa gtcgcctcag ggcgttcgcc gtcgcttgga aatattcgtg aggtggatcg
       61 gccggaggaa tcacttgggg agcaatattc agcaggtcgg ctaagagttg tgcggtgtct
      121 ttggcccgta gccacgctga acgattaatg attcacgaga aggtccacca ttgacggttc
      181 aaggcaaaca aagagcaaag gccttggccg atacgctgcg cgatgccggg gttaccgcaa
      241 tcttaacttc acagtggcta cggccaaaga caccgcgcag ccactagcca acctgctgaa
      301 tattgctccc caagtgattc ctacggccga tccacctcac gagtatttcc aagcgacggc
      361 gaacgccgta aggcgactta aggatgagac ggttctagtc gtcggacaca tcacgatccc
      421 caacatcatc gccgagtttg gcggtccgca actcccgaca atctgtgagg gcgtgttctc
      481 ggatttgttc ctgctgattc cggctctggg acggaaggcc tcacaagact gcgatttggc
      541 ccttaggagg agatttcgcc gcgctgcaga gatctgcctt gattgcgacc gcatccagac
      601 gacctatgat gagataacga cagattttgc tcgtcatgtt aattcgtcgc aacggtttga
      661 catcgtccga ctcttattgc ttgaattgaa agagcatgga aacgcggtct gacaccagct
      721 cagcgctatc aacccctcaa aggaaccaat cgcaatccca ggggattcct acaagtggtc
      781 aatcctatgg tcgggagctc gccgggagaa tgctgccgcc gattctggcg attgcgagcg
      841 tgctcgctgt gtggtgggga ttgtatctcg tttttccccg gttactgcct ggtccggtga
      901 gcacgtttaa cgaagctatt cgcttgttgt ccgatggcac tttctttttt cacatgctgc
      961 agagcctgcg acgggttttt gtcggcgccg cagtcgccat gatcttcagc atcgctatcg
     1021 gtatttatat gggtaccgtt ctcatgggcg agcggttttt ccaaccgctg gtggtcttag
     1081 gactcacgat tccgggtttg atgtgggccc tgattgcggt gatgttgttc gggattaacg
     1141 agatcgcttc ctactttgca gtggcggtca ccacgtttcc gatgctggtg atcaacatct
     1201 gggccggcgt aaagtcgctc gataaagagc tcatcgacat gagccacgtc tttcgtttca
     1261 caccctggat gaagatctct caggtgatca ttcctcagct cgtgcccaac atttttgccg
     1321 ccacccgcta cggtctgggt ctggcgtgga aagttgttgt ggttgtcgaa atgttcggca
     1381 ccagcaacgg cgtcggctat caagtgatga aatcgtacca agttttcaat atggaggggg
     1441 taatcgcctg gacgcttact tttgtcgccg ccatgatcat cattgaatat ggcatcatca
     1501 atctggccga gcgccgcctc acggcctggc gaccaaaggt cgacgtttgg aggcgttgag
     1561 tggctcggtt gatcgttcgc gatgtggaaa agtggtttcg gcgcgaggac gggcagccat
     1621 taaagattct cgatcgtctt tcgtttgagg cgctagaagg ttgcgtcacg gtggtggtgg
     1681 ggccttccgg ctgcggcaaa tcgacgctat tgaacgccat cgtcgggttg gaacggctgg
     1741 accgaggtga actggcgttt gcaaccgagg acgttcattt gcaacgccgt ccgctcattg
     1801 gttacgtttt tcaaagtcct cgactactac cctggaaaac cgtcggcgat aatcttaggc
     1861 ttgcgctgca aggcgccggt gtcgctcgag gtgagtggca ggagcgggtc gagcgctatc
     1921 tggatctggt tgggcttgga gaatacgcag cccagtttcc gctctatctt tcagggggac
     1981 agcgccagcg cgtagggctt gcccgagctt tggcgatcga atccgatctc gtgttgatgg
     2041 atgaaccttt cgcaagcgtc gatgagctga ccgcgcggca gttgcggcaa accacacgac
     2101 ggctttgcga acaattgcat cgtacagttc tttttgtcac tcataacttg cctgaagccg
     2161 cttatatgag cgattacgta gtgacgctca caacccgtcc agcacgactg gcggaatacg
     2221 tcactaatcc cctgcccgcc ccgcgaaatt ggactccgga gatctaccag tttgccgctg
     2281 agttggaagc caaagttact ttcaaagagg agagcgagtt atgaatctgt caattgtcat
     2341 ttcgtccatt gcaataatga tcacgctaag ctcattttgt cgcgcgccgg tacaagccca
     2401 gtcgcttcaa accgtcaaca tcgcaaccgc ggccgccggt caatctgggt atatcgccgc
     2461 gctgatcgac gccaaaggca tcgccgccaa acacggactg aagatcaaca atctgatgat
     2521 ggacttcacg gaagcagcca acgcattgaa gttgggacgg gcaatcgcta gcgccatgca
     2581 accgagcacc gcggtcaatc ttctcaagag cggcaccgat atacggctga ttgcgccaca
     2641 gatttggtcg ggcaattcct ggctggttaa aaaggattcg ccctataagt ccctggccga
     2701 tctcaaagga aaaaaaatcg gaaactttgc gcgggttacc ggagcctatt tcttctcggc
     2761 tgtaattgcc aaggagaaaa aactcgatat cgaaaaggac ttccagagta ttccagccga
     2821 gactggcgcc ctgatcgcgt tattggaacg aggcgaagtg gaagcgatca acatgttcga
     2881 gccccatacg acccggctgg tactctccga caaatatcgg gtactgctcg atttcgatgt
     2941 ggagctggaa aaactgttcg gcgcgccacc gctgaaatca tccgtcggca tgctcagaga
     3001 aacggtcgaa aagcagccag ctctggtgaa agcgattcgc gccgcatatg tcgaagcggt
     3061 caaaatgatc aaggccggcc aagacaagga ttttttccaa gccaaggcca aagaattttt
     3121 caatctcagc acgcctgaac aagtcgctgc agggatgaaa caaaatcgac aaaacttcgc
     3181 cgatctttgg ggtgagtcgt tctttcaatc acagaacaaa atcctacaat cgggaatttc
     3241 tttgggttta ctgccgaagg tgaacgtaac cgagctgtgg ataaaataac catgagcgta
     3301 aattaagtcg caacttgagg gctctttatc gggttctagc gtcggtttgc cataaaactg
     3361 ttgtgatgta agttgcttcc cgctgtttgc atttggccgt ggctggcgcg gcgcagatac
     3421 atcttgaccc agtagtcata aaaatattgg agattctgct tcgtttcttc aggatatcct
     3481 gcagcagtct cgaaacgaat cgttacccat cggtcttgga gagagattcc gattaaagag
     3541 ctattcttcc ggccgtcgag atacgctccg gcgcttcgca gtatatgtga caaactatag
     3601 ggatcgggca gcgggctgat gctggagcgc ttgcaacgag cgagatagtc ggccttgacg
     3661 atctcctcaa gatcatagcg tagattgata atgcgcgaac gcaaagacgg gcgaaaatcg
     3721 ccatgcagga tggcgcgtaa tcgtggcatg aaccggcgtg gaaggtaact aaggaagcgc
     3781 gaatcgccac tcgatttctg aatgccccgg acgatatagg catccgcaac gcagactaag
     3841 tcagctctag tgatttgccg ccgctcgagg agctggccca ccactctcag atctcgagcg
     3901 taagaagacg cgggcgtttg atctaaaccg tccatctgcc agggtgcttc ggcttcatca
     3961 tgcaagcttt cgccgggatt gatcagacta cttgctgttt tcattatcct agccctcaaa
     4021 tcttccccct tacgtttttc ggcgctggag attgcattgg atccagcagg cccaaattgc
     4081 cattcgctgg aactctgaac gcaagttgaa ggccatcagc cactagcgta gcgcttggaa
     4141 attcagtgac attattggtc gatcaagcga acagttgtgc cactggttca acttgtctga
     4201 catttccgac aattcacata ctgaggaact ctgtaattcg acgctcggca aaagggatgt
     4261 gaaataaacc gagtttgaga aggggggcga ttgcgctcta gagcatgaat ccaattttct
     4321 gatttctgcg ggtagtcttc acgctagttc cgtggtgccc atcaagaagc gatcgcattc
     4381 tcgtgcagca ccacgaccct cattgatggc ccacacgacc aggctctgac cacggcggca
     4441 atcgcccgcg gcaaagacgc cgggaacatt cgtagaaaac tccccatact cggccttagc
     4501 attcgaacgt ggatctcgct ccacgcccaa ttgggccaac accgtctcct caggaccgac
     4561 aaagcccatc gccagcaaga ccaaatcagc gcgtaatatt ttatcggtcc cgggtactcg
     4621 cttcggaatg aaggcgccct gctcgtttct ggcccattcc acatcgtata cgtgcagctc
     4681 ttttacccgc ccatcggaat cgccgacaaa tttctcgccg gttgttagat agatccgcgg
     4741 gtcggcgcca aaacgtgcct tcgcctcttc ctgaccgtaa tccaatttat acaccttggg
     4801 ccattcaggc catggattat ccggctggcg tgtatcgggt ggccgcggca gaatttctaa
     4861 ttgagtcaag ctcttgcatc cgtgtcgcat ggacgtgccg acgcaatctg tgccggtatc
     4921 accgccgccg acgacgatca catgtttgtc ttttgcggaa atgtatctat tgtccttgtg
     4981 attggaatcg agcaggctct tggtattggc gtggagaaaa tccatcgcga agtggatgcc
     5041 atcgagctcg cgccccggta tcggcaagtc acgcgctttg gtcgcgccg
//
LOCUS       DASUTG010000516         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1116396, whole genome shotgun sequence.
ACCESSION   DASUTG010000516 DASUTG010000000
VERSION     DASUTG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116396"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VF089_11860"
     CDS             <1..920
                     /locus_tag="VF089_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEX6174702.1"
                     /translation="IVAVLATLEVSLSFDNAVVNAAVLRKMSPLWRRRFITWGIAIAV
                     FGMRIVFPLVIVAIIARIGPVSALVLAATDPDNYSRVLTSANVSVSAFGGAFLAMVGL
                     RHFFNREKSVHWISIIERPLTRLGRIEAVELGLVLLLMYFVSTQLGGYDQFQCLVAGI
                     FGLVTYIAVDGISALLKVESTLTGEVVQSGAASFLYLEVLDASFSFDGVIGAFALSNN
                     LFIIAIGLGVGAMFVRSLTIMLVERETLTSYRYLEHGAFYAIIALAVMMFISAVQHIP
                     EAVTGLVGAGFIIAAFLDSIRFNRRLRGA"
     gene            complement(989..1285)
                     /locus_tag="VF089_11865"
     CDS             complement(989..1285)
                     /locus_tag="VF089_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174703.1"
                     /translation="MSENSKNPEEPFDHLVRTLQEGEAWRISQCIQDIDHRLLDCRKS
                     LEEYRRLRSTLRTINNKLSRLGAEPLKVAVELAGQDLSEIIKSRLDHFKSCGKI"
     gene            complement(1401..>2130)
                     /locus_tag="VF089_11870"
     CDS             complement(1401..>2130)
                     /locus_tag="VF089_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174704.1"
                     /translation="SLRRIKQRITLHHRLYSLQTPTEVDHYIRHRLDIVGYSGPEIFT
                     REAVEAIWQYSGGTPRLINILCDNTIGIACLKPKKKVSAYMIMKAAGGLLLEPGVEAP
                     KVSADSGFLRMKTAAPRTSPRRSETNVGESTVKDRALTPPTPIPLRESDRLKLSRIAT
                     KGPAVSLQFFDRMTRAATEAMGPMANRIVVDQISALGESRQAFPQGKLAELILRISVE
                     ILNESMRDNFQRTMVREIATLKTM"
BASE COUNT          407 a          573 c          587 g          563 t
ORIGIN      
        1 tcattgttgc cgtgctcgcc accctcgagg tatcgctgtc gttcgataac gcggtggtta
       61 atgccgctgt cttaaggaaa atgtctccgc tatggcgacg ccgtttcatc acctggggca
      121 ttgccattgc tgttttcggc atgcgcatcg tctttccgtt ggtcattgtt gccatcattg
      181 cgcgaatcgg tccagtttcg gccctggtgc tggctgccac cgatccggac aactattcgc
      241 gggtgctaac cagcgcaaat gtctcggttt cagctttcgg cggcgctttt ctggcgatgg
      301 tcggactccg gcactttttc aaccgggaaa aaagcgtgca ctggatctct atcatcgagc
      361 gcccgctaac gcggctcggg cgcatcgagg cggtggaact ggggcttgtc ttgctgctta
      421 tgtacttcgt ttccacccag ctcggcggtt acgatcaatt ccaatgtctg gtcgcgggga
      481 tcttcgggct cgtcacttac attgcagtag atggtatttc ggcgctgttg aaggttgaat
      541 cgacgctgac gggagaagtg gtgcagtccg gcgcagcttc gttcctttac ctcgaggtgc
      601 tcgatgccag tttcagcttt gacggcgtga tcggcgcctt tgcgctatcg aacaatcttt
      661 ttatcattgc catcggcttg ggcgtcggcg cgatgtttgt ccgcagcctg acgatcatgc
      721 tcgtggagcg cgaaacgctc acctcttacc ggtacctgga gcacggcgca ttctatgcca
      781 tcatcgcatt ggcagtgatg atgtttatca gcgcggtgca acatattcct gaagctgtca
      841 ccggactggt cggagcagga tttattatcg ccgcctttct cgattccatc cgctttaacc
      901 gccgcctccg aggcgcttag ccgtcgccta aatcaatatc ggcttaatcc aaggccgagt
      961 aaaaagcgtc ggcaattcct gaggcggcct aaatcttacc gcaggacttg aaatgatcga
     1021 gccggctctt gatgatctcg cttaaatctt ggccggctag ctcgacagca accttcaacg
     1081 gctcggcgcc cagtcgagaa agcttgttgt tgatggtgcg aagagttgag cgcagacggc
     1141 gatattcttc aagcgacttc cgacaatcca gaagcctgtg gtcgatgtct tgaatgcatt
     1201 gactgatcct ccaggcttcg ccttcctgta gtgtgcgcac cagatgatcg aacggctctt
     1261 ctgggttttt agagttctct gacatttaat ctcccttccc ttagaactga tcattatagg
     1321 ttggcaactt gcggcagtta atctggtagc gactgcgata cgtccccatt gccgatagat
     1381 gcctacctcg agacaatttt ttacatggtt ttgagagtgg cgatttcccg caccatggtt
     1441 ctctggaaat tgtctcgcat gctctcgttc aggatttcta cgctaatccg caatatgagt
     1501 tctgcgagct ttccttgggg aaatgcttgg cgggattcgc cgagtgcgga aatctgatcc
     1561 acgacgatgc gattagccat cggccccatc gcttctgtcg ctgcgcgagt catgcggtca
     1621 aaaaactgca gcgaaacggc aggtcccttt gttgctatcc gggaaagttt gagccggtcc
     1681 gactcacgca gcgggattgg cgttggcggg gttagagctc gatccttcac ggtgctctca
     1741 ccaacgtttg tttcagagcg ccgtgggctt gtccgcggcg ccgcggtttt catcctgaga
     1801 aatccgctat cggcggaaac ctttggcgct tccacgccgg gttccaacag cagcccgccg
     1861 gcggctttca taatcatgta agcagaaact ttcttcttag gcttcaggca agcgattccg
     1921 attgtgttat cgcaaaggat attgatcaac cgcggcgttc cgccggaata ctgccagatc
     1981 gcctcgaccg cttccctggt gaaaatctca gggccggaat agccgacgat atcgaggcga
     2041 tggcgaatgt aatgatcgac ctcggtgggc gtctgcaatg agtagagacg gtggtgcaag
     2101 gtaattctct gtttgatgcg gcgcagagat
//
LOCUS       DASUTG010000517         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_228335, whole genome shotgun sequence.
ACCESSION   DASUTG010000517 DASUTG010000000
VERSION     DASUTG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228335"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF089_11875"
     CDS             <1..206
                     /locus_tag="VF089_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174705.1"
                     /translation="HRELAETRQKVAAALPALNRVQRVVLPRAEQDREPELAEALLDR
                     EAVDQLEAAPKAVEAALGKRILD"
     gene            complement(351..656)
                     /locus_tag="VF089_11880"
     CDS             complement(351..656)
                     /locus_tag="VF089_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174706.1"
                     /translation="MKRSEPGLLQGFFLCLRDSDAIRLQSSKWKRNLLTFSSGICLEV
                     AAVPYLLGWLGYEATWQAWLLTWLFFPLGVTGAYASIFGGDRLVEWLLIVPKLDLKI"
     gene            complement(843..1232)
                     /locus_tag="VF089_11885"
     CDS             complement(843..1232)
                     /locus_tag="VF089_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX6174707.1"
                     /translation="MAIRIVRLGTTRNQDEGLRIGTVRRPPRGVRKEDYSKLGFYDVW
                     MPDLAPSTGLVSWANSEPFTPKRWSLFERRYRHEMRQPAPQRIITLLAALSSQTSFSV
                     GCYCEEEARCHRSILRQLLLEHGAKLA"
     gene            complement(1314..1886)
                     /locus_tag="VF089_11890"
     CDS             complement(1314..1886)
                     /locus_tag="VF089_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX6174708.1"
                     /translation="MSFADYRCEYQLMCTVITHPAVPIALSALLPQETASSSLLIAAA
                     LCSVIPDLDVVGFEFGIKYDDMLGHRGFTHSIFFAALLAALITFTWFQNSQGSGLIVF
                     MFLFLSTLSHPLLDALTNGGLGVGLFAPFSNERYFFPYRPIEVSPIAVGAFFSHRGVT
                     VILSEVKWVWLPSTIFFGLGHLLKRLHDLS"
     gene            complement(1883..2617)
                     /locus_tag="VF089_11895"
     CDS             complement(1883..2617)
                     /locus_tag="VF089_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6174709.1"
                     /translation="MRLWVGTSGYSYKEWRGKFYPPRLPAKEMLRFYASRFAAVEINN
                     SFYRLPKETVLQSWSEQVPADFRFVLKAPRRITHTKRLKDAGAGVEDLFSVAAALGSR
                     QGAILFQLPPNFKKDVERLRTFLSLLPADRHVAFEFRHPSWLVEEVFAMLRARNCALC
                     VADTDESESCELINTASWGYLRLRRRDYRAADLLHWKERISSQAWDHAYVFFKHEDEA
                     TGPKFANKLLQIGGDVPHNRLVSMKG"
     gene            3202..3528
                     /locus_tag="VF089_11900"
     CDS             3202..3528
                     /locus_tag="VF089_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174710.1"
                     /translation="MAAPVPDLSRSSAYPLSLSLAFALIVLSLWRVDTGLVEQQSATS
                     DGDIAMTSVAPAQDDSRYYRRAASLEDLAFGSWGTIPIESENEEVRRKATVALMVYAT
                     GNGIRR"
     gene            3812..4184
                     /locus_tag="VF089_11905"
                     /pseudo
     CDS             3812..4184
                     /locus_tag="VF089_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061346.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
BASE COUNT         1042 a         1102 c         1111 g          929 t
ORIGIN      
        1 tacatcggga actggcggag acgcggcaaa aagtagcggc ggcgctaccg gctctaaatc
       61 gagtccagag agtggttcta cctcgagcgg aacaggatcg ggaaccggaa ctggcggaag
      121 cgctactgga ccgggaagca gtggatcagc tggaagcagc tccgaaggca gtggaagcgg
      181 ctctggggaa gcggatccta gattagctct gcaaaataat aggtcgcacc gttgcgcgac
      241 tattcctttt taggcgtttc catttcatat gtgggactgt atccgagctg atccggcttg
      301 gtgaagtgtc ggccctgcaa cagaggtcct gtgcgcacgg cgaaagccgt tcaaatcttc
      361 aaatcaagtt tgggaactat caacagccac tcgacaaggc gatctccacc aaagatgctt
      421 gcgtaggctc cagtaactcc caatgggaag aatagccatg taagaagcca tgcctgccaa
      481 gttgcttcat agccaagcca accaagcaga tacggcaccg cggcgacctc gaggcagatg
      541 ccgctgctga aagttaaaag attccttttc cacttactcg attggagacg tatcgcatct
      601 gagtcgcgca ggcacagaaa aaacccttgt agcaaacctg gttcggaacg cttcatcttt
      661 gtggaacctt tcaatgatat ttgtgattgg atgccgtcgg ccggtggatc gcacgaggcg
      721 caatgttaag gcgctagcga cgagaatgca aattcctcgg ctgaaataac agagattttg
      781 cggagacgtg aagtgcgctt tacgtcaagg tgggatgttc gacggcgggg aggccggtgt
      841 gctcaagcta acttggcgcc atgctcaaga aggagttgtc gtaaaatcga acgatgacag
      901 cgcgcctcct cttcgcagta gcagcctact gaaaaactcg tttgtgatga tagtgctgcg
      961 agcaacgtga taatgcgctg cggcgccggt tggcgcatct catgccggta gcggcgctcg
     1021 aacagagacc agcgcttcgg ggtgaacggc tcggaattag cccatgacac cagcccggtg
     1081 ctgggcgcca aatccggcat ccagacatca tagaagccca gcttggaata atcttccttg
     1141 cgaacgcctc gcggcgggcg cctgacagtg ccgatgcgta agccctcatc ctgattacgg
     1201 gtagtcccca gccggacgat gcgaattgcc atagatcgag tgttcttgcg ggaaatccct
     1261 ctcgtttcga ggcaagaaag tcacatcagt gacggcttgc acacaaccat tgctcaactc
     1321 aggtcatgca aacgcttgag tagatgcccg agcccaaaga agattgttga cggcaaccaa
     1381 acccatttca cctcgctcag gatcactgtt actcctctgt gcgaaaagaa agcgccgact
     1441 gcaatcggcg aaacctctat ggggcggtaa ggaaaaaagt atcgttcatt gctgaatggg
     1501 gcaaataaac cgactcccaa gccgccgttt gtcagcgcat ccagcagcgg gtgcgacaat
     1561 gttgataaaa agagaaacat gaaaacaatc aagccgctgc cctggctgtt ctggaaccac
     1621 gtaaacgtaa tcaacgctgc aagtagcgca gcaaagaata ttgagtgagt gaagccccgg
     1681 tgccccagca tatcgtcgta tttgattccg aactcaaaac cgacaacgtc cagatccgga
     1741 atcaccgagc aaagcgcggc agcaatcagc aaggaagacg aggcggtttc ttgtggaaga
     1801 agtgcggata aagcgatggg tacagcagga tgagttatga ctgtacacat gagctgatat
     1861 tcgcaccgat aatccgcaaa gctcaacctt tcatcgatac cagacgatta tgcgggacat
     1921 ctccgccgat ttgcaggagt ttgttcgcga atttgggtcc ggtggcctcg tcttcatgtt
     1981 tgaagaaaac ataggcatga tcccaagcct gcgaagaaat tcgctctttc cagtgcagca
     2041 ggtcggcggc tctgtaatcg cgacggcgca gccgcagata accccaactc gccgtattga
     2101 tcagctcaca gctttcgctc tcgtcggtat cggccacaca caacgcgcag ttgcgcgctc
     2161 gcagcatcgc aaagacctcc tcgaccagcc aggatggatg ccggaattca aaggccacat
     2221 gccgatcggc gggcagaagt gataggaacg ttcgcagccg ctcgacgtcc tttttgaaat
     2281 tgggcggaag ctgaaacaga atggcgcctt gccttgatcc tagagctgcc gctacgctaa
     2341 acagatcctc aactccggct ccggcatctt taagcctctt cgtatgggtg attcttcgtg
     2401 gtgccttgag cacaaaccgg aagtcggcag gcacctgctc tgaccacgac tgaagaaccg
     2461 tttccttggg cagccggtaa aaactattgt tgatttcgac agcggcaaat cgcgaggcgt
     2521 aaaagcgcag catctccttg gcggggagcc gcggaggata aaacttgccg cgccattctt
     2581 tgtagctgta gccgctggtc ccgacccaga gcctcacgtg cggtcgagcc ttctactata
     2641 atctttcaat gctatggcgt gcggcatcaa gttattttta tcatagatcg gggctagagc
     2701 gagcgctgtt aattcgtata tgggtgggag gccgctaaaa cgagcgcgag ccatcgcgca
     2761 aggtgcccgt gtgctccgta gaaggatggg cgcaatcgtc ccacagtcct tcttcgaata
     2821 ccaagacata actctttaaa ctgtgatctg tcagtatcat tctcattagc ggctaagaga
     2881 aacgctgacg gtagacatcg aagtgagagc acagcccgga attcggtaag cacccccgac
     2941 ctgctcagcc ttagcagcac aaacgcgagt cagaggattc acctgaatgt caaatccctg
     3001 tcagctgcac gattgcgtcg tggtctttgg ccggcagaac acacctttag catcactctt
     3061 ctcagtaacc gttcaaaccc gcctggaacc tacgtcaact ttattttgct gattccgtct
     3121 aaatggattg tgtttctctg gtttggtgct agcaagagac tagagtgcaa aaatcctccc
     3181 ctaagtgaca aggagatatt tatggcagct cctgtcccgg atctgtcccg cagttcggct
     3241 tatcctttat ctctatctct ggcctttgcg ctaatcgtgc tatcgctctg gagagtggat
     3301 acgggtttgg tcgaacagca atcggctacc tcggacggcg acatcgccat gaccagcgta
     3361 gcgcctgccc aagacgactc acgctattat cggcgcgcag cttctcttga ggaccttgct
     3421 ttcggttcat ggggaacgat tccgattgaa tcagagaatg aagaggtccg acgcaaagcg
     3481 accgttgcat tgatggttta cgctactggc aatggcataa ggagatagcc aagcgcatgt
     3541 tctctgcttg gaccaaactc gaaggttcct gaaggttcac ggcagcttat tctctcggac
     3601 agcagaacct ggcgcgtgtt aaatccctga actcgatcgc gggcctaagc tcacatcgag
     3661 cataccccag tccctcatct cttctcgaca atacgtcact cgagtgactc gccacggtaa
     3721 ttctcagttc ttcgctcacg tgctttgaca accacttcga cctggtgcgg ccagtcattg
     3781 ttgatgtacc attggccgta aggagatcaa catgaagaat ctgctcgcgt ggtttacgga
     3841 atttttgaag tgcatagccg aagtggcagg ccacccggtc acttttagtt tcgcgctgct
     3901 caccatcatt atctggagtc acagggccgt tgtttgggta cagcgacact tggcagcttc
     3961 tgatcaacac cggaactaca atcgtcactt ttctgatggt atttctcatc cagaaaacgc
     4021 agaaccgcga cactcaggca ctacacttaa agcttgacga gttaatcaga cacccaagga
     4081 gcgcgtaaca tgatgctcga tctagaacat atggatgatg aaagtttaaa ccggctacgt
     4141 gagatttacg agaggttggg cactcaagcg agaggcgagg aaaa
//
LOCUS       DASUTG010000518         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1265189, whole genome shotgun sequence.
ACCESSION   DASUTG010000518 DASUTG010000000
VERSION     DASUTG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265189"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VF089_11910"
     CDS             <1..305
                     /locus_tag="VF089_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6174711.1"
                     /translation="AAVAEAAGLPCNVNGSVETGVGNAANLHLAASTGVVTYGCVVPV
                     SSPTEKAKKGIAGIYYQDDIISEPFAFDNGDIIVSPKPGLGIELDEDKLEHYRIKS"
     gene            390..1523
                     /locus_tag="VF089_11915"
     CDS             390..1523
                     /locus_tag="VF089_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX6174712.1"
                     /translation="MAIRKIAVLGAGNGGCAAAADLTLRGYEVRLFSRSDATLLPIIK
                     QGGITLLEAGEEKFAAPYFHSPHIVPVIDGVDLIIVAAPAVAHEYLAQSMAAHLRDGQ
                     RILLNPGHTGGSLHFANILRLAGSKANVRLCETVTLTYICRMIKPAHVEIYRRTTNLR
                     CAAFPARDNDDLIGEIQSIYPNVTVAQNVLETGLSNINAIMHPAGMLGNAGWIENTAG
                     NFLYYRQGVTPAIAAWIEEIDKERLQIVKSLGLKLIPFIDIFYQAGLTSAEAQASGSV
                     YRAIHESEPNFKIRSPACLDHRYLHEDVGYGLVPMAEIGRLFGIKTPVMDSLITLASV
                     ANGVDYSREGLTLEKLGLAHIAAENLLAILQKGFGVPNGRYPD"
     gene            1593..2576
                     /locus_tag="VF089_11920"
     CDS             1593..2576
                     /locus_tag="VF089_11920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174713.1"
                     /translation="MRLGLCFDGFYSVQEMIELARLADEAGMESLWMSDHLCFRDSLT
                     TSMALLASTQRIKVAPAPMSPYSRAPIISAMSLATMEEFAPGRVIASPGTGNAAALKE
                     AGIESPRPLKTMREYVDILRRLLKGDSVDYQGEMFQLNGPKMGFAPSTPIPMYVTAVR
                     PRMLQLAGEIGDGVLLSGGCSPAYIARCVTEIQNGAAKAGKVVSNVDVAGFITAAVSE
                     KPREAIDANKLFLAYIFRNTHHAENIRLGGGKVDQEGLAAAVGKRDWDAAKKYISDEV
                     VFAHSLAGTADDCRRQLQAFISGGLNLPVLLPMGTQEMRKSVIRMAAGLVA"
     gene            2863..3885
                     /locus_tag="VF089_11925"
     CDS             2863..3885
                     /locus_tag="VF089_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174714.1"
                     /translation="MGSLMQDWKISLSVVCAIAWLSAGNALAQQPNIEAAKKEGRVVV
                     YGAQVPQAMKPLHAAFEKKYGISVEYWRGSSTGVSERALTEWRAGKPGFDVVEGNRGV
                     QLIMRDDGLFQKYVPPSSEKFPAQFKEKDGMITPWRVLPISILYNTDMVKPGELPKTF
                     DDLATAKWAGKITMPDPTRHTTTAQFLWNLKEFKGDKWLDLVKALASQRPLLVESLAP
                     VTTTIIKGEAPVGITYIKYVKQYKGPVGYVLMDKYLSDPNYMSLSAKAANPNAAKLYI
                     EFVTSAEGQKLVAEEGEFVLYPGVLPPIKDAEKVAPNMIFMDNPSEEEFKKLMTGTFR
                     EIFFNR"
     gene            4016..>4286
                     /locus_tag="VF089_11930"
     CDS             4016..>4286
                     /locus_tag="VF089_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174715.1"
                     /translation="MDRPLRAFRKNPELAVVLVVGIVIAYLSLSPTLMLFYGSFRSTP
                     LGVPGDFTLGHYLGAYTDAQTYQLLLNSFVFASGSAILSTVLAATL"
BASE COUNT          971 a         1112 c         1220 g          983 t
ORIGIN      
        1 ttgccgccgt tgccgaagcc gcgggattgc cctgcaatgt aaacggatca gtagaaacag
       61 gagtcggcaa cgcagccaat ctgcacctgg ccgcatcgac cggggttgtt acatacggct
      121 gtgtggtgcc ggtttcttcg cccacggaga aagcgaaaaa agggatcgca ggaatttatt
      181 accaggacga catcatcagc gaaccgttcg ctttcgacaa tggcgatatc attgtttcac
      241 ctaaaccggg gctgggcatc gaactggatg aagacaaact ggaacactat cgaatcaagt
      301 cctaattagc agattccaac cagctcgagc gtttcgggca tttcctagcg taagttctta
      361 gaattttgcg tcaagcatat tcgtgacgta tggcaattcg taaaatcgcc gtcttgggcg
      421 cgggcaatgg cgggtgcgca gcggcagctg atttgactct gcgcggctac gaggttcggc
      481 tgttttcccg ttcggatgcg actctgctgc cgatcatcaa gcagggtggt atcacgcttt
      541 tggaggccgg cgaggaaaag tttgctgctc cgtactttca cagcccacat atcgtcccgg
      601 ttatcgatgg agtcgatctg atcatcgttg cggcgcccgc agttgcccat gaatatctgg
      661 cgcaaagcat ggcggcacat ctccgggacg gccaacgtat tctcttgaac cccggccata
      721 ctggaggctc gctgcacttt gccaacattc tgcgtcttgc cggcagtaag gcgaatgtca
      781 ggctgtgcga gaccgtgacc ctgacctata tttgccgcat gataaaaccg gcccacgtgg
      841 aaatctatcg acgcacgact aatctgcgct gcgccgcttt ccccgcgcgg gacaacgacg
      901 atttgatcgg cgaaattcag agtatttacc ccaatgttac agttgctcag aacgtgcttg
      961 aaacgggttt gtcgaacatc aacgccatca tgcatccggc gggaatgctc ggcaacgcgg
     1021 gttggatcga aaacaccgct ggtaattttc tgtattaccg ccaaggagta accccggcga
     1081 tcgcggcgtg gattgaagag atcgataagg agcgcttgca gattgttaag agcctcgggc
     1141 ttaagctcat ccctttcatc gatatctttt atcaagcagg gctgacttcg gctgaagcac
     1201 aggccagcgg ttctgtttat cgcgcgatcc atgaaagcga gccaaatttc aaaattaggt
     1261 ctccggcctg ccttgatcac cgttaccttc atgaagacgt cgggtacggt ttggtaccga
     1321 tggccgaaat tggccggctc tttgggatca aaacgccggt aatggactcg ttgattacct
     1381 tggcgtcagt ggctaacgga gtcgactact cgagggaagg tttaactctg gaaaaactcg
     1441 ggcttgcgca tatcgcggcg gaaaacttgc tggctatttt gcagaaaggt tttggcgttc
     1501 ctaatggtcg gtacccagac tgatcgcacg aaacacgatg gcatacgagg ccacgaaggc
     1561 tgcgaaggcg taaaggtagg ggaggtgccc ttatgcgatt gggcttatgc tttgacggat
     1621 tctattcagt tcaggagatg atcgagctcg cccgtcttgc cgatgaggct ggcatggagt
     1681 cgctttggat gtccgaccat ctctgcttca gggattcgct gacaacctcc atggctctgc
     1741 tcgcttccac ccaacggatc aaggtcgctc ccgcgcccat gagtccttat tcgcgcgcgc
     1801 cgatcatctc cgccatgtcg ctggcgacca tggaagaatt tgcgccagga agggtgattg
     1861 ccagcccggg aactggaaac gctgccgcgc tgaaagaagc cggcatcgag tcgcctcgcc
     1921 cgttgaaaac gatgcgtgaa tacgtggaca ttcttcgtcg attgttgaag ggtgactcgg
     1981 ttgattatca gggcgaaatg ttccagctga acggtccgaa gatggggttc gcgccatcaa
     2041 cgccaattcc aatgtatgtc actgcggtgc gtccgcggat gctccagcta gcgggtgaaa
     2101 tcggcgatgg cgtcttgctg tcgggcggct gttcgccggc atatatcgcg cgttgcgtta
     2161 ccgaaattca gaacggcgcc gcgaaagcgg gcaaggtggt tagtaatgta gatgtggcgg
     2221 gttttattac tgcggcggtt tcagagaaac cacgtgaggc aatcgatgcg aataaacttt
     2281 ttctcgcgta tatctttcgc aacactcacc acgccgaaaa cattcggttg ggcggcggca
     2341 aagtcgatca ggagggattg gcagcagcgg tgggcaagcg agattgggat gcggcgaaga
     2401 aatatatcag cgatgaggtg gtttttgccc actcactcgc cggcactgcc gatgattgcc
     2461 gacggcagct gcaggcattt atcagcggcg ggctgaactt gcccgtgctc ttaccgatgg
     2521 gcactcaaga gatgagaaaa agtgtcatcc gcatggccgc tgggctcgtc gcctgaaagc
     2581 ggcggttggt ggtgaatcgg cctaaaaaat ctacgaattg agacccatcc ccaatccggc
     2641 gatacgaatt cactgcagca gccgtgagac tgacaccttc tactccggct ttgtgtcgat
     2701 tctctaggtg cccgggatca ccgttgttac gtgaggtagg gcatgaggtg acctaagtgg
     2761 cggcgaaata aggaaatgag aaatcatctc gtttcttgac ttaggggctt tcgatatata
     2821 cttattacct gcctaattcc tctcataaat aaaggaggct ccatgggttc actgatgcag
     2881 gattggaaaa taagcctgag tgtcgtgtgc gcgattgctt ggctgtcggc cggcaatgcg
     2941 ctggcacagc aaccaaacat cgaggcggcc aagaaggaag gcagagtcgt tgtgtatgga
     3001 gctcaggttc ctcaggcgat gaaaccgctc catgcggcgt ttgaaaagaa atacgggatc
     3061 tcggtcgaat attggcgcgg ttcgtccact ggggtttcgg aacgggcgct cacagaatgg
     3121 cgcgccggca aacccggctt cgatgtcgtc gaaggcaatc gcggtgttca gctgatcatg
     3181 cgcgacgacg gactgttcca gaagtacgtc ccgccgtcgt cggaaaagtt tcccgcgcag
     3241 ttcaaagaga aagatggcat gatcactccc tggcgggtcc ttcccattag cattctgtac
     3301 aataccgaca tggtcaaacc gggcgagttg ccgaaaacat tcgacgatct tgcaactgcc
     3361 aaatgggccg gcaagattac catgcccgat cctacgcgcc acacgacaac ggcgcaattt
     3421 ttgtggaatc ttaaagagtt caagggagac aaatggttgg acctggtcaa agcgctggcc
     3481 agtcaacgac ctctgctggt cgaatccctg gcgccggtga cgaccacgat catcaagggc
     3541 gaggccccgg tgggcattac ctacatcaag tatgtcaagc agtataaagg tccggttggt
     3601 tacgtgttga tggacaagta tttgagcgat ccaaactata tgagcctgag tgcgaaggcg
     3661 gccaatccaa acgccgctaa gctctacatc gaattcgtca cttcggctga aggtcaaaag
     3721 ctggtcgccg aagaagggga gttcgtgctc tatcccggag tgttgccacc catcaaggac
     3781 gcggaaaagg tcgcgcccaa tatgatcttt atggataatc cgtccgagga ggagttcaag
     3841 aagctgatga ccggtacctt ccgggagatc tttttcaaca gataaccagg catcgcttgt
     3901 acgatgaggt agagggctgg ccctgcgtga agcgtttgcg tggccagccc tcttgctatt
     3961 tatgactcca gtcaaaggta acgccttaac agattcctca actccgagcc ggctgttgga
     4021 tcgaccccta cgggcttttc ggaaaaatcc ggagctcgcg gtggttctgg tagtcgggat
     4081 cgtcatcgct tacctcagct tgtcacccac cctgatgcta ttctacggca gcttccgcag
     4141 cacaccattg ggcgtgccgg gcgacttcac cctcggccac tatcttggcg cctacacaga
     4201 tgcgcagacc tatcagctgc ttttaaactc atttgtattt gccagcggtt cggcgatcct
     4261 ttcgaccgtg ttggcagcca ccctag
//
LOCUS       DASUTG010000519         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_228906, whole genome shotgun sequence.
ACCESSION   DASUTG010000519 DASUTG010000000
VERSION     DASUTG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228906"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF089_11935"
     CDS             complement(<1..801)
                     /locus_tag="VF089_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6174716.1"
                     /translation="MELANEQTGAARLSEEVARRRTFAIISHPDAGKTTLTEKLLLYG
                     GAVETAGAVRAGRNQRHATSDWMELEQERGISITASALEIEYQGFRLNLLDTPGHQDF
                     SEDTYRTLMVADSAVMVLDSAKGIEAQTEKLFQVCRMRGIPILTFINKLDHFGRDPLE
                     LMDEIERVLGIATAPMNWPIGMGSTFQGVYDLQHRRVLQFERTERGQWRAPVAVRSLD
                     DPALPALLSETLSGELREHIALVTGAGNAFDRRRFLAGDLTPVFFGSAL"
     gene            complement(1025..1861)
                     /locus_tag="VF089_11940"
     CDS             complement(1025..1861)
                     /locus_tag="VF089_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6174717.1"
                     /translation="MRTLVHLSDLHFGTVRQEIVLPLLSLLERIQPDLIAVSGDLTQR
                     ARKEQFAAARDFLDAMPFPKIVVPGNHDVPLYNLFARFALKLTRYRRYISDDLEPFYS
                     DPEIAVLGINTARSATFKGGRVNSRQVARMQERLGSFNSVTKIVVTHHPFDLPEAYPD
                     KALVGRARRAMQQLAGSGIDLFLAGHLHIGLSEPTALRFQIGGRSPLMIQAGTALSSR
                     VRGEANSFNVIRIEHPNISVSRLVWRADLKMFVSSHADYFRHTEAGWARGNKLDFAEP
                     ED"
     gene            complement(1883..2767)
                     /locus_tag="VF089_11945"
     CDS             complement(1883..2767)
                     /locus_tag="VF089_11945"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6174718.1"
                     /translation="MIVIVNVGARKADDTEVHQRLVEIFTARGLRPEIQLARSGAEIL
                     DLARRATKAESSVVVAAGGDGTVNAVGSELVGSNKILGVLPLGTLNHLAKDLGMPLDL
                     ESAVDVIAQQRLAQIDIGEVNGRYFLNNSGLGLYPGIVHEREQQQAQGRSKWLAFFSA
                     LLIIIHKYPLMTVRLSTEGRELRRRTPMVFVGNNEYELQGLNMGSRRFLNRGKLFLYV
                     TREMSRWRLLKIGLAALFGRLSDSRDFDAMSVREVSVQARRRRLRVALDGEVVVMRLP
                     LRYRIRPAALRVIVPAEE"
     gene            complement(2963..>3351)
                     /locus_tag="VF089_11950"
     CDS             complement(2963..>3351)
                     /locus_tag="VF089_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174719.1"
                     /translation="LLQKGSYVPLAVSGPAAGRVCVFARRDGAVWSLVAVPRLPTGVF
                     SADHVPLGRQGAWEDTALELPGDSPAIWNHVFTGETLPARKTADGERYLYLKDLFCDF
                     PVALLEAGSTSSDAMPAFLSLEERLQ"
BASE COUNT          748 a          988 c          906 g          709 t
ORIGIN      
        1 gagtgcgcta ccgaagaata caggcgtgag atctcctgcc agaaaccggc gccggtcaaa
       61 cgcgttgcct gctcctgtca cgagcgcaat gtgctcacgc aattcaccac tgagagtttc
      121 gctcaacagc gcaggaagcg ctgggtcgtc caggcttctg acggcgaccg gggctcgcca
      181 ctgcccgcgc tcggtccttt cgaactggag aacgcggcga tgttgcaggt cgtatactcc
      241 ctggaatgtc gatcccatac caatgggcca gttcattggc gcggtcgcga ttcccaaaac
      301 ccgctcgatc tcgtccatca gttccaaagg atccctgccg aagtgatcta gtttgttgat
      361 gaaggtcaag atcggaatgc cacgcatccg gcagacctgg aaaagcttct ctgtttgagc
      421 ttcgatgcct ttggcgctat cgagcaccat gactgcgcta tccgcaacca tgagggtgcg
      481 gtaggtatct tcgctgaaat cctggtgccc cggcgtgtct agtaggttga gtcggaaccc
      541 ttggtactcg atctccaggg cgctggcggt aatcgaaatc ccccgctcct gctccaactc
      601 catccaatcc gaagtggcat ggcgctgatt tctaccggcg cgcaccgcgc ccgccgtctc
      661 cacagcaccg ccgtatagta aaagtttctc tgttagggtc gtcttgccgg catcggggtg
      721 cgagatgatg gcaaaagttc ggcggcgcgc cacctcttct gacaagcgcg ccgcgccggt
      781 ttgttcattt gcaagttcca tggttccttc ttcgggttaa taaacgctgg ccaggatggt
      841 ttcgacggga ccaatttcgg tccgaacacg cggccaaaag gacctagcaa tttcttaact
      901 gtgacgggtt catatcagtc attacattca aacagagcag tatacagtag tctcgtgcga
      961 tacgcgattt gaggcaggca atgtagttcc ttgcttcggt ctacggcaga aacgtttcgt
     1021 cccatcagtc ttccggctcg gcgaaatcca atttgttgcc acgcgcccaa cctgcctcgg
     1081 tgtggcggaa atagtccgca tgactcgaaa caaacatctt cagatcggcc ctccacacca
     1141 gtcggctcac cgagatattg ggatgctcaa tacgaatcac gttgaatgag ttcgcctctc
     1201 cacgcacccg cgatgataga gctgtgcctg cttgaatcat cagcggcgag cgtccgccaa
     1261 tctgaaagcg taaagccgtc ggttcgctca ggccgatatg cagatggccg gcgagaaaca
     1321 gatcgatccc tgagccggca agctgctgca tcgctcggcg ggcgcgaccg accaatgctt
     1381 tatccggata agcctcaggc aaatcaaaag gatgatgcgt cacgacgatt ttggtcacgc
     1441 tgttgaaaga tccgagccgc tcttgcatcc gcgcgacctg tcgagagttg acccgcccgc
     1501 ctttgaaagt tgccgaacgc gcggtattga tccccaaaac cgcaatctca ggatcgctat
     1561 agaatggttc aagatcatcg ctgatgtaac gcctgtatcg ggtgagcttc agggcgaagc
     1621 gggcaaacag gttatacagc gggacgtcat gattgccggg aactacgatc ttcggaaacg
     1681 gcatggcatc caagaaatct cgcgcagcgg caaattgctc tttgcgtgct cgctgggtca
     1741 gatcgcctga caccgcgatt aggtccggct ggatccgttc gagcaagctt agcagaggca
     1801 agacaatctc ttgacgcacg gtgccgaagt gaagatcaga gaggtgaacc agagtacgca
     1861 tggatttcag cggccgattt ctttattcct ccgcaggcac aattacgcgc agagcagccg
     1921 gacgtatgcg ataacgcagc ggcagtctca tcaccaccac ttccccatcc aacgccactc
     1981 gcaagcgtct ccggcgcgcc tgtacggaaa cttctctgac cgacatcgca tcgaagtcac
     2041 ggctgtcgct caatctgccg aacagcgcag ccaagcctat cttgagaagc cgccagcggc
     2101 tcatttcgcg cgtgacatac aaaaagagtt ttccacggtt gagaaatctt cggctgccca
     2161 tgttcaagcc ctgcaactcg tactcgttat taccgacaaa gaccatgggc gtgcgacgtc
     2221 tcagttcgcg accttcggtg ctgaggcgca ccgtcatgag cgggtactta tgaatgataa
     2281 tcagcagggc ggaaaagaaa gccagccatt tactccggcc ctgcgcctgc tgttgttcgc
     2341 gctcatgaac gatacccgga taaagtccca ggccggaatt attcaggaag tagcggccgt
     2401 tcacctcgcc gatatcgatc tgtgccagcc tctgttgcgc tatgacgtct acggctgatt
     2461 cgagatcgag aggcataccg agatcctttg ccagatggtt gagcgttcct aaaggtaaaa
     2521 cgcccagtat cttgttgctg ccgacaagtt ccgaacccac ggcattaacg gtgccgtcgc
     2581 cgcctgcggc aacgacaaca gagctttcgg ccttggttgc acgccgagcc agatcaagga
     2641 tttcggcgcc gcttcgcgcc aactggatct ccggtcgcaa acccctcgcg gtaaatattt
     2701 cgaccagccg ctgatggact tcagtgtcgt cggcttttcg cgcgccgacg ttgacgataa
     2761 caatcacgtc cgacgccata gtgctaagat aaccaaacag ttttaatcga cggtaagagc
     2821 gacgtggtgc gaatcttttc gcacgctctc agcccttcat tgtttgggag ttgaggttca
     2881 gtattgacgg gtcgcgggtg tgagaacgtc gggagaaatc ttccccagat cgcatccccg
     2941 actcgctcgc ggcgcccgcg gctcactgca aacgctcttc gagcgacaag aaggccggca
     3001 tggcgtccga cgacgtggaa ccggcctcaa gcagcgcaac cgggaaatcg caaaaaagat
     3061 ccttcaaata taagtatctt tctccatcgg ctgttttacg cgccggcagc gtttctccgg
     3121 tgaacacatg attccatatg gcggggctat cgcccggcag ttcaagcgcc gtgtcttccc
     3181 aggcaccttg ccggcccaaa ggcacatggt cggcagaaaa gacaccggta ggcaagcgcg
     3241 ggaccgcgac caaactccaa accgcgccat cgcgcctggc aaaaacacac acgcggcccg
     3301 cggcggggcc ggatacagcc agcggaacat agctgccttt ttgcaataac t
//
LOCUS       DASUTG010000520         5800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_377045, whole genome shotgun sequence.
ACCESSION   DASUTG010000520 DASUTG010000000
VERSION     DASUTG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377045"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VF089_11955"
     CDS             complement(<1..882)
                     /locus_tag="VF089_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174720.1"
                     /translation="MIGNSLEIWKLKTYLRKVAMTDSHVLITGETGTGKELAAQYIHQ
                     HSTRRAKPLVTINCAALPDGLLESELFGYERGAFTGAVSAYAGKLKHADGGTVLFDEI
                     GEMSPYAQAKILRVIESREVYPLGGRRSMPVNIRIMAATNRDLDREMAANAFRQDLYF
                     RLNVARIKLPPLRERKEDIPLLVDYFAQKFSEQFGRQIQGFTDEALDLLKRNDWPGNI
                     RELMNLIERIFIDPPMGKINVGDLPEPMRSPGGSRQSAIPEERELLLSALSQTNWNKS
                     QAAERLHWSRMTLYRKMA"
     gene            complement(1418..2596)
                     /locus_tag="VF089_11960"
     CDS             complement(1418..2596)
                     /locus_tag="VF089_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174721.1"
                     /translation="MLKFIKHEGGCIGAILFSSLHGVLRHLRCINNHANGGMMGPIRS
                     KLFIISVFMFLFSALNAAPAITLPELTTRVEKLSRAEREALLIKGAKEEKEVMFYATT
                     PVNQVAVLKKAFSARYPFVDLKHYYSPRQGILNKTVSEYRGKANITDVVMTDVSYGTI
                     LIKEGVSHPYTTPDVQRYVRGSYDPKGYWYTMYMLTTALVYNRNLVKPGEAPKTYQDL
                     LDPKWKGRMMFDPEAAFIMAAMEEVWGKEKARDYLARLSKQDLIFRRGTTLTSQLIAA
                     GEHPIAIAVNAETTAEMRDKGAPLGFSVLPPRIIKPNGFFVAKNAPHPHAALLFTDWS
                     LSEEGQKVLAVDLGKGVAMKGIPAKYKEFQGDPDYVVGPEFGNQLKQHMTEFQKIFGM
                     "
     gene            complement(2813..4156)
                     /locus_tag="VF089_11965"
     CDS             complement(2813..4156)
                     /locus_tag="VF089_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174722.1"
                     /translation="MGDSSTDVNPPDAGGPIYRLLGRFLDVRPGETRALGWSWLYIFS
                     VLSSYYILRPVRDDMGVAGGIENLPWLFTGTLAGMIAVNPPFAALVARLPRVRFIAIA
                     YRFFIANLLIFAVLLYAASAEQNIWLGRIFFIWTSIFNLFVVSVFWALMVDVYDSEQS
                     KRLFGFIAAGATLGGIAGSSITASLAKQISPVYLLLISAALLEVAVFGVRRLSRLSQA
                     LSRQPAMRSDEAPIGGSVFSGVTHALKSPYLLNVSVYILLFTITSTFLYFQQAEIARQ
                     SFADRGARTAFFASIDLWVNILTLGAQLFLTHRLLRRVGVAATLAILPALSVLGFAAL
                     ASMPTIAVLVAYQVLRRAGNFAFARPTREVLFTVVPREDKYKAKSFIDTVVYRLGDQV
                     GAWSYAGLGLLGLGMTGIALVAVPISCAWLMNSWWLGRKQEKMVKEPAKVSLETL"
     gene            complement(4198..5124)
                     /locus_tag="VF089_11970"
     CDS             complement(4198..5124)
                     /locus_tag="VF089_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6174723.1"
                     /translation="MGISRRDFLKAGIVAGTALALPAKSPFAQPTTLLQKKIPSTGES
                     IPIIGIGTARRYEEIQTATEKVPLKATVQRFKELGGKVIDSSPTYGTAEAVVGELVDE
                     LKARDALFLATKVSLRDSGREAGIKQIEQSFKRLRTDKIDLLAVHNLRDTETQLRTIR
                     EMKQAGRVRYVGITTSFEKQYPEFEQTVKKETLDFIQVDYALDNRNAGERIIPLAGDR
                     GMAVMINLPFGRGRLFNAVQGKKLPEWAAEIDCSSWAQFFLKYIVSHPAVTCAVPGMA
                     KPEYVSDNLGAARGRLPDAAMRRRMEMFIDAA"
     gene            complement(5379..>5800)
                     /locus_tag="VF089_11975"
     CDS             complement(5379..>5800)
                     /locus_tag="VF089_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX6174724.1"
                     /translation="EVLVMEKVLASFAPYAYAFMRIVVGLLFVCHGGQKVFGWFGGQP
                     VPLMSLFGVAGIVELILGVLITIGFLTSYAAFIASGEMAVAYFMAHYPKSFWPLENEG
                     EPAVLFCFVFLYIATQGAGVWSIDGESAVRRRRQTFR"
BASE COUNT         1375 a         1646 c         1509 g         1270 t
ORIGIN      
        1 cgccatcttg cggtagagtg tcatgcgcga ccaatgtaat cgctccgccg cctggctctt
       61 gttccaattg gtttgggaga gcgcagacaa caacagctca cgctcttccg ggatagcgct
      121 ttggcgagag cccccgggag atcgcatcgg ttccggcaaa tcgccaacgt tgatcttgcc
      181 catcggcggg tcgataaaaa tgcgctcgat caaattcatc agctcacgaa tattgcctgg
      241 ccaatcgttc ctttttaaca aatccagcgc ttcatccgtg aagccttgga tttgccggcc
      301 gaattgttca ctaaatttct gcgcaaaata atctaccagc agcggaatat cttcctttcg
      361 ctcccgcagt ggcggcagct tgatcctggc gacgttgaga cgaaaataga gatcctggcg
      421 aaatgcgttg gctgccatct cacgatccag gtcgcggttg gtcgcagcca tgattcgtat
      481 gtttaccggc atgctgcggc ggccgccgag cggatagacc tccctgctct ctatcacccg
      541 aagaatcttg gcttgcgcgt aggggctcat ctcgccgatt tcgtcgaaca gcactgtgcc
      601 gccatcggca tgtttcaact ttcccgcata ggcggaaacg gcgccggtaa aagcgcctct
      661 ctcatagcca aacaactcgc tttccaatag tccatccggg agagccgcgc agttgatcgt
      721 aaccagcggc ttggcgcgcc gcgtactgtg ctggtggata tactgagcgg caagctcctt
      781 acccgtcccg gtctcgccgg taatcagcac gtgactgtcg gtcatcgcca cttttcgcag
      841 gtatgttttg agcttccaaa tttccaggct attcccgatc attgatcttc cgagcatggg
      901 tgccgtcata gcttggactc cgggcgacct ttgaacattt cccgacgctg agtgagcgcg
      961 tcggttgtcg agactgcggc agttttacgc cggatttatt ttttgactta gtacagtctt
     1021 tatcattcgg tcaagcagtt tttgtcgatc gttaataacg cttggtgatc ttaacgttcg
     1081 ttaaagcaaa tgcgagaccg aatgcgaaga gtcgcgggac gatccgaagc gcgaactttt
     1141 cggaattttt ctaccgaaga tcaattttca aaaaagcccg caactttaga gacacactgc
     1201 gcctcacaat agtctatgga tgaaggagca ggctccatct gagggaaatt atgaaatctc
     1261 cagaattgga gcgtgaagca ggtgtggtta gcggctgatc aaaaaggatc gcaggtgcaa
     1321 ggcgcgcgca gatcgctgag cggagacgta ctccggtagt acgtcgaaac gaaacgagca
     1381 agcgcaacga cgcagatgtg cctttttcat cagccgctca catgccgaaa atcttctgaa
     1441 actccgtcat gtgctgcttc aattgattgc cgaattcggg gccgacgacg taatcggggt
     1501 cgccctggaa ctctttatat tttgccggta tgcctttcat cgcgacccct ttacccaaat
     1561 ccacagcgag aaccttttgc ccttcctccg acagtgacca gtccgtaaag agcagcgcag
     1621 cgtgcggatg gggcgcattc ttggcgacga aaaatccgtt cggtttgatg atccgtggcg
     1681 gcagcacgct gaagccaagc ggcgcgcctt tgtcgcgcat ctcggcggtg gtttcggcgt
     1741 ttaccgcgat ggcaatcgga tgctcgccgg cggcgatcag ttgcgaggtt aaagtcgtgc
     1801 cgcgcctgaa tatcaaatcc tgctttgata gcctggcgag atagtcgcgg gctttttctt
     1861 ttccccagac ttcttccatc gccgccatga tgaaagccgc ctcggggtca aacatcattc
     1921 tccctttcca tttgggatcg agaagatcct ggtaggtctt cggcgcttca cccggcttga
     1981 ccaggttgcg gttataaacc agcgcggtgg tcagcatgta catcgtgtac cagtatcctt
     2041 tgggatcata ggagccgcgc acgtaacgct gcacatcagg cgtggtatac ggatgcgaaa
     2101 cgccttcctt gatcaggatt gtgccgtagc tgacatcggt catgaccaca tcagtgatat
     2161 tggctttgcc gcggtattcg ctgaccgtct tgttgagaat tccctggcgc ggcgaatagt
     2221 aatgtttcaa atcgacgaat ggatagcgcg cgctgaaagc cttctttaac accgccacct
     2281 gattgaccgg ggtggtggcg taaaacatga cttctttttc ttccttggcg cctttgatga
     2341 gcagcgcttc acgctcggcg cgactcagct tctccacccg agtggtcagc tccggcaggg
     2401 tgatcgccgg cgcggcgtta agcgcgctaa acagaaacat aaaaacacta atgatgaaca
     2461 attttgatct gataggaccc atcatccctc cgttggcatg attgttaatg cagcgcaggt
     2521 ggcgcaacac tccatgcagg ctgctaaaca aaatagctcc gatgcaaccg ccctcgtgtt
     2581 taataaactt aagcatcagt caggctgcat cgacgatcta tgaaaatact ggtatggagc
     2641 accgctttgg cagcaaaaat cattctgcaa cttacataga tcaccgaccc gcttgaagca
     2701 agttatgcct cgacagcgat cacagatctg actgagccaa attgatgccc gaatgcgcct
     2761 agcttgcgac acgcgaaaga attacaggcg actatcctgc cgcagtattg atctagagag
     2821 tctctaatga aaccttcgca ggttccttga ccatcttctc ttgcttgcgg ccaagccacc
     2881 agctgttcat caaccaggca cacgagatcg gtaccgcgac cagagctatg ccggtcattc
     2941 ccaatcccag cagtcccaaa ccggcgtagg accacgcgcc gacctggtcg ccaagccgat
     3001 acaccacggt atcgatgaaa cttttcgctt tgtacttatc ttcacgagga accacggtaa
     3061 acagcacttc ccgggtcggg cgcgcaaacg cgaaatttcc ggctcggcgc agcacctgat
     3121 aagcgaccag caccgcgatg gtcggcattg acgccagagc ggcgaaaccc aaaacgctta
     3181 acgccggcag gatcgccaat gtagccgcaa ctcccacgcg acgcaaaaga cgatgagtca
     3241 aaaataattg cgctcccagt gtcaaaatat ttacccacaa gtcgatacta gcgaaaaaag
     3301 ctgtgcgcgc tcctcgatca gcaaagcttt gacgggcgat ttccgcttgc tgaaagtaca
     3361 gaaacgtcga agtgatggta aacagcagga tgtaaacact cacgttgagc aagtacggcg
     3421 acttgagcgc gtgagtaaca ccggaaaaga ccgagccgcc gataggcgct tcatcggaac
     3481 gcattgccgg ttgccggctc aacgcctgcg acagccttga taagcgccgc actccgaaca
     3541 ccgcgacctc aagcagtgcg gctgaaatga gcagcagata aacgggcgat atttgtttgg
     3601 ctaacgaagc ggtgatgctc gacccggcga taccgccgag agttgcgccg gcggcgataa
     3661 aaccgaagag ccgtttgctc tgctcggaat cgtatacatc gaccatcaac gcccagaaaa
     3721 ccgacacgac gaaaagattg aaaatcgatg tccagataaa gaagatccgg cccagccaaa
     3781 tgttctgttc agcgctggcg gcataaagca gaaccgcgaa aatcaacaaa ttggcgataa
     3841 agaatctgta agcgatggca atgaagcgaa cccgcggcag ccgagccacg agcgctgcaa
     3901 agggcggatt caccgcgatc atgccggcca gagttccggt aaaaagccac ggcaggtttt
     3961 caatgccgcc ggcaacgccc atgtcgtcgc gcactggccg gagaatataa taagaggaga
     4021 gtaccgaaaa tatgtacaac catgaccacc cgagcgcccg ggtttcaccc ggccggacat
     4081 cgagaaaccg cccgaggagc cggtaaatcg gaccgcccgc atccggcgga ttgacatccg
     4141 tcgacgaatc acccatcacc ggctccccca gggacctacc gatcgccggg ctagcgatta
     4201 tgccgcatcg ataaacattt ccatgcgacg gcgcatcgcc gcatcgggca gacgacctct
     4261 cgcagcaccc aaattgtcgc tgacgtactc gggcttagcc atgccgggga cggcgcaagt
     4321 gacggcggga tgtgaaacga tgtatttgag aaaaaactgc gcccatgagc tgcaatcaat
     4381 ttccgctgcc cattcaggta atttcttccc ctgcaccgca ttaaacagcc ggccgcggcc
     4441 gaatggcaga ttgatcatga ccgccatgcc tcggtcgccg gctaacggta taatcctttc
     4501 cccggcattg cggttgtcga gagcgtagtc gacttggata aaatcgaggg tttccttctt
     4561 taccgtctgc tcgaactccg ggtactgctt ttcgaaagaa gtagtaatgc cgacataacg
     4621 aactcgcccg gcctgtttca tctcacggat ggtacgcagc tgggtttcgg tgtcacgtaa
     4681 gttatgtacc gcgagcaagt cgatcttgtc ggtgcgcagg cgcttgaagg attgctcgat
     4741 ctgtttgata ccggcctccc ttccgctatc gcgcaagctt actttggtgg ccaagaacaa
     4801 ggcatcccgg gccttgagct cgtcgacgag ttcacccact accgcttcag ccgtgccgta
     4861 ggtaggcgag ctgtcgatta ctttgccgcc cagctcctta aaccgctgaa ccgtggcctt
     4921 gagaggcact ttctcggtgg ccgtctgaat ctcctcgtaa cgccgggcgg tgccgatgcc
     4981 gattatggga atgctctcgc cagtggaggg aattttcttc tgcaacaggg tggtaggctg
     5041 ggcgaacggg ctttttgccg ggagcgccag cgctgttccc gcaacgatcc cggccttaag
     5101 aaaatcacga cggctgattc ccattagaca atctcctaat ctgcctttag gcaggttgct
     5161 gaagaagacc catatgcttc gttgtgctca aatcgcctcg cctctggatc tttttgagca
     5221 gcctgcaacc agaatttttc acctgttagt tcgccctgtg gcaacctgcg atcgattgct
     5281 aagcgagtcg caagcgagtt tataactatt taactagacg gcaacacgcg gcgcggcgga
     5341 cttcacttat taaaccgttc gatttcgatc cgcgaccgct atctaaaggt ctggcggcgg
     5401 cgccggacgg ccgattcgcc atcgatgctc cagactccgg ctccctgggt tgcgatgtac
     5461 agaaatacaa agcagaagag cacggccggc tcaccttcat tctctaaggg ccaaaaactt
     5521 ttggggtagt gagccatgaa ataagcaacg gccatttcgc cgctcgcgat aaacgcagca
     5581 tagctggtga gaaatcctat agtgattaga acaccgagaa tcagctcgac gattcctgcc
     5641 actccaaaca gcgacatcag cggcaccggc tgcccgccga accagccgaa aaccttttgc
     5701 cccccatgac aaacaaacaa cagtccaacg acgatccgca taaacgcata ggcatacggc
     5761 gcaaacgaag caagaacctt ctccatgacc aagacctcct
//
LOCUS       DASUTG010000521         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1561926, whole genome shotgun sequence.
ACCESSION   DASUTG010000521 DASUTG010000000
VERSION     DASUTG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561926"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF089_11980"
     CDS             complement(<1..459)
                     /locus_tag="VF089_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6174725.1"
                     /translation="MAETNDERVPVTVLTGFLGAGKTTLLNRILTAEHGRRVAVIVNE
                     FGEVGIDHHLLISSEQEIVEMSNGCICCTVRGDLLRSLFQLLEHKSKFDTLMIETTGL
                     ADPAPVVQTFFVDERIKSQFQLNGVVTVVDSKHIALQIDHSTEAREQIAFA"
     gene            593..1228
                     /locus_tag="VF089_11985"
     CDS             593..1228
                     /locus_tag="VF089_11985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174726.1"
                     /translation="MIEFRPIGIEQYVERHSKPMSLFHDKIWLETYRQTGNPTTMVGP
                     LAAQLLKMLVLMTGARRILEIGMYTGYSTLAFAEALPKDGRVVTCEINPETTAIARQC
                     FAESQHGRKIEVRLGPAMNSLKVISGPFDLCFIDADKDRHGEYFDASMELVRSKGVIV
                     LDNMLRSGKVMHPSDPGSQAIDALNKRIRNDTRVENVLLPVRDGIMLVVKL"
     gene            complement(1262..2158)
                     /locus_tag="VF089_11990"
     CDS             complement(1262..2158)
                     /locus_tag="VF089_11990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6174727.1"
                     /translation="MNQELVVPAAETDKKLQTFLKSRFPIGYVRKLFRKNGLRVNGKR
                     SKPDDLIRAGDRIRLYIPFEKTIRHRDTAPLGPPKFSVIYEDPKLLVINKPAGIAVHE
                     GKRVLKRHSVIGALESTYRKQGLTPRLVHRLDKDTSGVLLIAKDELTTEEMQAILADG
                     RDVEKEYLCLVAGRLQDNQGRIDLPLPGREGAPVRALTDFKVLDRFSQTTLARVNIET
                     GRMHQIRLHFAAIGYPVVMDDQHGDFNFNKQFSKKYGLKRQFLHASRLEIKHGGKKRV
                     WSAPTPPDLQKVIEALRVDKAD"
     gene            2301..>3253
                     /gene="pheA"
                     /locus_tag="VF089_11995"
     CDS             2301..>3253
                     /gene="pheA"
                     /locus_tag="VF089_11995"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX6174728.1"
                     /translation="MSRNESVDVLRKKIDQIDEKVVALLNDRAFLAQKIGRSKSQNNQ
                     EVYVPHREKQILQRLSRLNQGPLPEHSIRAIFREVVSACRSIEAPLKVAFFGAEGTYS
                     HLAAREKFGSTLTLLPTTSISEVFQEVSQERVSFGVVPIENSTEGVVAHTLDVLVDSE
                     LKICAEIYLEIHHNLLSRTGREQDIKRIVSHPQALAQCRQWLIRHFPAAKLDEVASTA
                     HAAMIAAGDKSVGAISSALAKEAYDLRVIAANIEDHSNNITRFLVIGGQETLPSGDDK
                     TSLVFSVKDKPGVLHRMLQPFARTRINLTKIESRPIKNKPW"
BASE COUNT          755 a          813 c          885 g          800 t
ORIGIN      
        1 ggcaaaagcg atttgttctc gggcctcagt tgagtgatcg atttgtagcg cgatatgctt
       61 ggaatcgacc accgttacga ctccattgag ttgaaattgg ctttttattc gctcatcgac
      121 gaagaacgtt tgtacgaccg gcgccggatc ggctagtccg gtggtttcga tcatcagtgt
      181 gtcgaactta gacttatgtt cgagaagctg gaataagctg cgcagcaggt cgccgcgaac
      241 ggtgcagcag atgcagccgt tgctcatttc gacaatttct tgttccgagg aaatcagtag
      301 gtgatggtcg atgccaactt cgccgaattc attgacgatg acggcaacgc gccggccgtg
      361 ctcagcggtc aatatacggt tcaacagtgt tgttttcccg gcgccgagaa atccggtcaa
      421 gacagttact ggaactcttt catcatttgt ttcggccatc gacttgaaat cctttcacaa
      481 ttgttttgta aaggtaacac acacttaggc cgagtcaact ttgatgcgct aacggtatcg
      541 tctctgcctg ttacgagcga cggagcgcgt gagtgcgtaa ggaggaaaga ctttgattga
      601 gtttagacct ataggcatcg aacaatacgt cgaacgccac tctaaaccga tgtcgttgtt
      661 tcatgacaag atctggttgg aaacctatag gcaaacagga aatccaacca ccatggtcgg
      721 ccctctggcg gctcaactct tgaagatgct cgtgctcatg accggcgcgc gccgcatctt
      781 agaaatcggc atgtacacgg ggtacagcac cttggctttt gccgaagctt taccgaaaga
      841 cgggagggtc gtcacctgcg aaatcaatcc tgaaacgacg gcaatcgcaa ggcagtgttt
      901 tgccgagagt cagcacgggc gaaaaatcga agtgagacta ggacccgcta tgaattcgtt
      961 gaaggtgatt tcaggcccat tcgatctttg ctttatcgat gccgacaagg accgtcatgg
     1021 tgaatacttc gacgccagta tggagttagt tcgatcaaag ggtgtgatcg tcttggacaa
     1081 catgctgcga agcggtaagg tcatgcatcc cagtgatccc ggcagtcagg ccatcgatgc
     1141 gttgaataaa cgcattcgca acgacacgcg ggtggagaac gtcctgttgc cggtgcgtga
     1201 cggcatcatg ctggtggtaa agctctgagg tgacgttaag attataaaac aagagcccca
     1261 tctaatcggc tttgtcaacg cgtaaagcct cgatcacttt ctgcaaatcc ggcggcgtcg
     1321 gcgcgctcca gacgcgcttc ttgccaccat gtttgatctc aaggcgcgag gcgtgcagaa
     1381 actgccgttt taggccatac tttttgctaa attgtttgtt aaagttgaag tcgccgtgtt
     1441 gatcgtccat cactacggga taaccgatcg ctgcgaagtg aagccggatc tgatgcatgc
     1501 gcccggtttc gatgttcacg cgcgccagcg tggtttgtga aaatcgatcc aatactttga
     1561 aatctgtaag cgccctcact ggcgcgcctt cccgaccggg taacggtaaa tcgattcgac
     1621 cttggttatc ttgaaggcgt ccggcgacca gacagagata ttccttctcc acgtctctgc
     1681 catcggcaag gattgcttgc atttcctccg tcgttaattc gtctttggcg atcagcaaga
     1741 ctcccgaggt gtctttatct agacgatgca ctaatcgggg ggtgagtccc tgttttcgat
     1801 atgtgctttc gagcgcaccg atgaccgaat ggcgcttcag gacgcgctta ccttcgtgaa
     1861 cggctatgcc ggcgggcttg ttgatcacca gcagcttggg atcttcgtaa atcacggaaa
     1921 acttcggtgg tccgagtggt gcggtatcgc ggtgccggat ggttttctca aacgggatgt
     1981 acaggcggat gcgatcgccg gcgcgaatga gatcatcggg cttggaacgt ttgccgttga
     2041 ctcgcaaacc gtttttccga aaaagtttgc gcacgtagcc aattggaaac cggctcttca
     2101 ggaaagtttg tagtttttta tcggtttcgg ccgcaggcac gacgagttct tggttcaccc
     2161 aggtcaatct gcggcacgct tttgagttcg tcaagcgaat atttgagcaa tatttgcaat
     2221 tctttaggaa ttaagatagg ttacgccggt atttcttaaa agagtctgcg cgccgggtgc
     2281 gcgccccagc gaggttgtct ttgagtcgta atgagtcggt cgatgttctc cgcaaaaaaa
     2341 tcgatcaaat tgatgaaaaa gtcgttgccc tgctaaacga tagggccttc cttgcgcaaa
     2401 aaatcggccg gagcaagagc caaaacaatc aagaagttta tgttccgcat cgtgagaagc
     2461 aaatcctgca gcgactctct aggttgaacc aaggtccgct tcccgaacac tccatccgcg
     2521 ccattttcag agaagttgtc tccgcttgtc ggtccattga agcgcccctt aaggtcgcct
     2581 tcttcggcgc cgaaggaacc tattcgcatc ttgctgcccg ggaaaaattc ggttccacgt
     2641 taacgctgct gccgacgacg agtatcagcg aagtgtttca ggaggtgagc caggagagag
     2701 tgtcgtttgg ggttgtgccg attgagaact ccacggaggg tgtggtggcg cacactctcg
     2761 acgttttagt cgattccgag ctaaagatat gcgccgagat ttatctcgag attcatcaca
     2821 atctgttgtc ccgcaccggg cgtgaacagg atatcaagcg catcgtgtct catcctcaag
     2881 ctctcgccca gtgccggcag tggttgatcc gtcatttccc ggccgcaaag ctggacgagg
     2941 tggcaagtac cgcgcacgcg gcgatgatag ctgcaggcga taaatcggtt ggcgcaattt
     3001 ccagcgcgct ggcgaaggaa gcatacgatc tgcgagtcat tgcggccaat atcgaggatc
     3061 actcaaacaa tatcacacgg ttcctggtca tcggtggtca agagaccctt cccagcggcg
     3121 atgacaagac gtctctggta ttctcggtga aagacaagcc aggggttttg caccgaatgc
     3181 ttcagccgtt cgcgcgaacg cgcatcaacc tgaccaaaat cgaatcgcgg ccgattaaaa
     3241 acaagccatg gga
//
LOCUS       DASUTG010000522         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_674315, whole genome shotgun sequence.
ACCESSION   DASUTG010000522 DASUTG010000000
VERSION     DASUTG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674315"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VF089_12000"
     CDS             <1..1038
                     /locus_tag="VF089_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174729.1"
                     /translation="AQSVFGKTYEEVLDRAKREAELRLWWEVPGERGVGEQFEAAFKK
                     RFGFVPRVHLTPVTNPDSLLRFLTEARVGRIDADLLYAGAADIAGRPDRLALFEDIET
                     PLVKIFSPKFKGLEGVLRNVAAWKRPFSVDVTTLASGIIYNTKKTNANDLPKTYEEFA
                     EGKGFADPKWKGNFAINTIGPASPLTDMGVGGHWDIEKQKKVLQMLLANKPLIKRSSG
                     DVRMAVALGEVAAATGNIGGTEGLRKEGYPIQNKLFEDVMALGSIGLTIPKGAKSPNL
                     ALLYLAFILEDGLPIVERVNGEGTFVDPRSQLAKSLKAMPKAKVLEWSPEEVLAGQRD
                     KIRKSLQPLMP"
     gene            1112..2212
                     /locus_tag="VF089_12005"
     CDS             1112..2212
                     /locus_tag="VF089_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174730.1"
                     /translation="MLTVEGLTRTFVTGDGQVQAVHDVSFEVKTGEFFSLLGPSGCGK
                     TTTLRCIAGLETPDSGEIWIDDKPVFSSKKGIMVPVHEREIGMVFQSYAVWPHMTVAQ
                     NVSYPLVHGRRKLPRNETKKRVKEILHLVQLDDLEARPSSLLSGGQQQRVAVARALVA
                     EPQLLLMDEPLSNLDAKLRVEMRHELRDLFERLKLTALYVTHDQEEALVLSHRMAVMH
                     GGRVVQLGPPMDIYKEPANLFVAGFVGTTNLLEGRVVGADGAGERGLVETAVGKLRCA
                     LPQQVVQGQRVSLMFRPEAVSIHTAACGSPVNVLPGHVRAVVFTGNRIEYDIDVGQYH
                     VRADTNPYVAPLEKGQSVWLEMPADRIRVLAA"
     gene            2253..>3015
                     /locus_tag="VF089_12010"
     CDS             2253..>3015
                     /locus_tag="VF089_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6174731.1"
                     /translation="MNFVDLTMPLNHRWMPDEVLPTAIKFFLGPKNHQEKGMVVGSDS
                     GTSLALPALFADFRKTTRLDQVPIEKLFLRPAAIANINKGAGEEINKADAQQAFDAAS
                     PAKGNALLVVTGWGDKPHHHAEGNEYVLSSPHFSLEAAHYLARAMHDNDNDLLLVDTA
                     VLGLPQSHIIPEWFSMLPAPAAESPEARMYLHLYDGEKAKKDFAVDMEFARMGIMTVR
                     KLIGCGQIRSRRIKMIVSPLNVVRGVGSTCRVVAVE"
BASE COUNT          680 a          773 c          957 g          605 t
ORIGIN      
        1 gctcaatcag tgttcggcaa gacctatgaa gaggttctcg acagagccaa gcgcgaggct
       61 gagttgcggc tgtggtggga agtgccgggc gagaggggag tcggagagca gttcgaggca
      121 gcgttcaaaa aacggttcgg tttcgtgccg cgggttcatc ttacgcccgt gaccaatcct
      181 gattctttgc tcagattctt gaccgaggcg agggtcggcc gcatcgatgc cgatttgctg
      241 tacgccggcg cggcggatat cgccggccgg cccgatcggc tggcgctgtt cgaagatatc
      301 gagacgccgc tggttaaaat attcagcccg aaattcaaag ggctcgaagg agttcttcgc
      361 aatgtggccg catggaagcg gccgttttcg gttgacgtga ccacgttggc atcgggcatc
      421 atctacaaca cgaaaaagac caatgccaat gacctgccga agacgtatga ggagtttgcc
      481 gaaggcaaag gttttgccga tccgaaatgg aagggaaatt ttgccattaa cacgatcggt
      541 cctgccagcc cgctcacgga tatgggcgtc ggcggtcact gggacataga aaagcaaaag
      601 aaggtactgc aaatgctgct cgccaacaag ccgctgatca agagaagcag cggtgatgtg
      661 cgaatggcgg tagcgttggg tgaagttgcc gcggctaccg gaaacattgg cggcaccgaa
      721 ggtctgcgca aggagggcta tccgatacaa aacaagctct tcgaagacgt gatggcgctc
      781 ggctcgatcg gcttgacgat tccgaaaggc gccaagagcc ccaatctggc gctactctat
      841 ctggctttca ttctcgagga tggcttgccg attgtcgaac gggtcaacgg cgagggaaca
      901 tttgtcgacc caagatcgca actcgcgaaa tcgttaaagg ccatgcccaa agccaaagtg
      961 ctggaatgga gtcccgagga ggtgctcgcg ggacaacgcg ataagatcag aaagtcactg
     1021 caaccgttga tgccctaaca ggctgctgaa aactctggtt acaggcgcat atgggtcttg
     1081 ttcagcaggc tgccaaggga agtcgtcgtc gatgttaacg gtagaaggac tgacacgaac
     1141 tttcgtcacg ggtgatggcc aggtgcaggc cgtgcatgat gtcagcttcg aggtcaaaac
     1201 cggagaattc ttttcgctgc taggaccctc cggctgcggt aagacaacca ctctcaggtg
     1261 tatcgccgga ctggaaacgc cggattcggg agaaatctgg atcgacgaca agccggtgtt
     1321 ttccagtaag aagggcatca tggtgccggt tcacgagcgc gaaatcggca tggtgtttca
     1381 gtcttatgcc gtgtggccgc atatgactgt ggcgcagaat gtcagctatc cgctggttca
     1441 cggccggcgc aagctgccga ggaatgagac gaaaaagaga gtcaaagaga ttctgcatct
     1501 ggttcaactc gatgatttgg aagcccggcc ttcctcgttg ctgagcggcg gacagcagca
     1561 gcgtgtggcg gtagcgcgcg ccctggtggc cgaaccgcaa ctgctcctga tggatgagcc
     1621 gttaagtaat ctggacgcca agcttcgggt ggagatgcgt cacgagctgc gcgatctatt
     1681 cgagagattg aagctgacgg ccttgtacgt gactcatgat caggaggaag ctttggtgtt
     1741 atcgcaccgc atggcggtga tgcatggtgg ccgggttgta caactcggtc cgcccatgga
     1801 catctacaag gagccggcca atctattcgt cgccggcttt gtcggcacga ccaacctgct
     1861 cgagggccgc gtcgtaggcg ctgacggcgc cggcgagcgg gggctcgtgg aaaccgcggt
     1921 gggaaagctg cgctgcgcat tgccgcaaca ggtcgttcaa ggtcaacggg tctcgttgat
     1981 gtttcgtccc gaggcggtat cgatccatac agcagcgtgc ggatcgccgg tcaatgtgtt
     2041 gccgggtcac gtgcgggcag ttgtgtttac cggtaatcgt atcgaatacg acatcgatgt
     2101 cggtcaatat cacgtccgcg ccgacaccaa cccatacgtt gcgccgctgg aaaagggaca
     2161 aagcgtttgg ctggagatgc cggccgatag aatcagagtg ctggcggcgt gacggatttg
     2221 ccggagcagc gccgcgagaa agtggaggaa atatgaattt tgtcgatctg acaatgccgt
     2281 taaaccatcg gtggatgccc gacgaggttt tgccaacggc gatcaagttt tttcttgggc
     2341 ccaagaacca tcaggaaaaa ggcatggtcg tggggagcga ttcgggaaca agcctggcgt
     2401 tgcctgcgct gttcgccgat ttccgcaaga ctactcggct cgatcaggtc ccgatcgaga
     2461 agttgttctt gcggccggcg gcgattgcca acatcaacaa gggcgctggc gaggaaataa
     2521 acaaggcgga cgcgcagcag gcgtttgatg ccgccagtcc ggcaaagggg aacgcgctac
     2581 tggtcgtcac cggctggggc gacaagccgc accatcatgc cgagggaaac gaatacgtcc
     2641 tgagcagccc gcacttctct ctcgaggccg cgcactatct ggcgcgagcg atgcatgaca
     2701 acgacaatga cttgctattg gtggatacgg cggtgctcgg actgccgcaa agccatatca
     2761 ttccggaatg gttctcgatg ctgccggcgc cggccgccga atcgcccgag gcgcgcatgt
     2821 atttgcatct ctacgacggc gaaaaggcca aaaaggattt cgccgtagac atggaatttg
     2881 cccgaatggg catcatgacc gtgcgcaagc tcatcggatg cggccaaatc agaagccggc
     2941 gcatcaaaat gattgtgtcg ccattgaacg ttgtccgggg cgtaggctcg acttgtagag
     3001 tcgtagccgt ggagg
//
LOCUS       DASUTG010000523         6590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1711356, whole genome shotgun sequence.
ACCESSION   DASUTG010000523 DASUTG010000000
VERSION     DASUTG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711356"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VF089_12015"
     CDS             complement(<1..348)
                     /locus_tag="VF089_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174732.1"
                     /translation="MERLKDRVAIVTGAAQGIGKALALGLSREGAKLVIADIHQDNAI
                     AVKDEICAAGAMAVAIATDVSDEMSVEAMVEHCIKEFGKIDILINNAGIFPVSSVEDM
                     QEEEWDRVIGTNLV"
     gene            complement(495..845)
                     /locus_tag="VF089_12020"
     CDS             complement(495..845)
                     /locus_tag="VF089_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174733.1"
                     /translation="MPRIRHLALVSPDRERLVNFYTTAFDMRVIYGRPKSTHLSDGDF
                     NLAIVDQGADLKAGLYVLGFDIDEMEDLEGCLRKAGASSDPQPMPKDHDAEYRVLDPD
                     GNPLDLSMHGWCVV"
     gene            complement(897..1244)
                     /locus_tag="VF089_12025"
     CDS             complement(897..1244)
                     /locus_tag="VF089_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174734.1"
                     /translation="MPKIRHIAIASSNRENLAAFYKQAFGMKEVKGEGGAIYLSDGSI
                     NLALNEQYAGREAGLYHFGFEVEDLDALKKKLKDAGAGSEVQRRPRNREAEYRVLDPD
                     GNPIDLSTHGWPT"
     gene            complement(1336..2385)
                     /locus_tag="VF089_12030"
     CDS             complement(1336..2385)
                     /locus_tag="VF089_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174735.1"
                     /translation="MKRMAVAWLAMVFGWTPNLLWAQPDFYRGKLITLVQDSTPGGVG
                     QLRTQALIPVLQKHIPGNPKIVVQFMPGAGGRKAANHLYSMAASDGLTIGRVGSGLVT
                     GAILGLPGVQYDLQKFIYLGSGQSEGSHVFFTRKEAGLDKLDKLKNAKGLRVGGQSVG
                     HSNYVVARLFAWLLDLKDPRFVVGFSGAEMDAALLQGELDARANSAETVVQRTPDFIK
                     QGMMHFHGVAEFPRGHRFSHPAFKDLPALHTLAKGDKEKSVVSMFIAFMQFSQAFVLP
                     PDSPNDRVKILKDAFRKSLNDPEFHDNWKKMTRAEASPLMPEELEELIKTIPRDPEVI
                     KLYNLIAGAEPLPRR"
     gene            complement(2419..2847)
                     /locus_tag="VF089_12035"
     CDS             complement(2419..2847)
                     /locus_tag="VF089_12035"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174736.1"
                     /translation="MGVEIFDLVELAHQEKRRKVVFNTQRCHAWVHYYPNPGERDDMH
                     CHNADQTFYVIDGECTMHFPDGGKAVMKPGMAATITGGSFYQLENSGTGPMIMMGNRS
                     GPSEAIQHINYELRKDIKKLSREELERAHIPHISMQTRGK"
     gene            complement(3020..4585)
                     /gene="ggt"
                     /locus_tag="VF089_12040"
     CDS             complement(3020..4585)
                     /gene="ggt"
                     /locus_tag="VF089_12040"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX6174737.1"
                     /translation="MGTRGMVATGHPLASDAALDVLKAGGNAIDAALCASAVLSVVKS
                     YHCGLGGDLFGIFYSANESRALVINGSGRSPRQIRRELFSKNIPHRGILAASTPGTVD
                     AWVEAAAKLGSRSMANLLEPAIHYAKTGFPVFPHLANVIRASQNSFGSDPAWSSIFLP
                     QGKAPEVGDLLVQKDLAITLAAIASGGRDEFYEGEIARSIVRASQYHGGCFSLQDFVG
                     HRSRWEEPLSTTYRGHEICVPPPNSFGLLLLLQLKRLSQYNPSNYGYNTPEFVALQVR
                     IKEEAWRAGQLWIGDPDEYDYEATLEFLKRFPIDYTATGTIPSADHGRSTTYVSVADE
                     LGNWASLIQSVHQSFGCGIVVDGTGIVLNNRMSGFNLIPGHRNELAPGKLPAQTLSPA
                     LVLRERRPILAIGTPGGLGQTQFLTQTLCNLFDFNMNIQEAIEAPRWQSEQPGQVELE
                     SRFSDDVGKLLLRQGYDVKLRGPWEFAFGGVESVFFASESGVHGSCGSKTRRLCDRIL
                     IDEYGIDYKICQS"
     gene            complement(4821..5168)
                     /locus_tag="VF089_12045"
     CDS             complement(4821..5168)
                     /locus_tag="VF089_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174738.1"
                     /translation="MARIRHLAIVTENRERLVKFYTTAFGMQVVDGVGPAIYLSDGYL
                     NVAIIQKRPHYKEGLYHFGIEVEDIEALKPLCKELGAATEVQKRPPNREAEYRVHDPD
                     GNPIDLSQHGWPV"
     gene            complement(5203..6177)
                     /locus_tag="VF089_12050"
     CDS             complement(5203..6177)
                     /locus_tag="VF089_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174739.1"
                     /translation="MKLRFVSFLWSMFLSLTGLGVVAAQELHMSYPGLTGESAPLWVA
                     REAGLFKAQGIDAQLVYMEGGRLSIQSLLSNTTQFMSGDAVSALSAIAGGADIVLLAS
                     AKNVLPYVFAVSKDVRGSQDLKGKIIATSQVGGRAGEIARMVIHNMGLDPDKDVNYLA
                     VGGTMSRLAALSTGKVHAAPISHTVVPIAEERGLQILKIDPIPLIVDALWTSRRFAEE
                     NPRIVQNVLRAYTQGIATLVKDRGRSIEIMRKYMRTSDPRVVQTAYDQYVQGLDRVPV
                     PSDKAIKNTLEISHRVAPKLASMDIKRHLYFAPVQKLAAEGFIDKLYK"
     gene            complement(6202..>6590)
                     /locus_tag="VF089_12055"
     CDS             complement(6202..>6590)
                     /locus_tag="VF089_12055"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174740.1"
                     /translation="ALKALSVRRVGLINPYPGELNDAVVAFMTAHGFEVQAVVSLGAD
                     FTRIGDVSEGDVYAAAKRAVQQSPRLEGLYLPCPQFPALDVIEAIESDFRIPAVGHLT
                     SEIWMALRTLKISSPVAGFGKLMRMV"
BASE COUNT         1549 a         1848 c         1565 g         1628 t
ORIGIN      
        1 gactaagttg gttccgataa cacgatccca ttcttcttct tgcatgtcct cgaccgaact
       61 taccggaaag attcccgcat tgttgataag aatatcgatc ttgccgaact ctttgatgca
      121 atgttcgacc atagcttcta ccgacatctc atcagagaca tccgttgcta tcgccacagc
      181 cattgcgccc gctgcacaga tttcatcttt gacggctatg gcgttgtcct gatggatatc
      241 agcgatgaca agctttgcgc cctcacgaga caagcccagc gccagcgcct taccgatacc
      301 ctgcgctgcc ccggtgacaa tagcgacgcg atcttttagc cgttccatct actgaactta
      361 attatgaagg atctttttga ggttcgagct cgactaatca gtccggcaat tctgccagga
      421 tgctctgttt ttttgatgcc aatatttaac ttgccgacag ctatgcgcct cgcgagcaac
      481 gcttagctta atagctagac aacacaccac ccatgcatcg aaagatcaag cgggtttccg
      541 tcaggatcta aaactcgata ttcagcatca tgatccttcg gcatcggttg aggatctgaa
      601 gaggcaccag cttttctaag gcatccttcc aaatcttcca tctcatcgat atcgaatccc
      661 aacacataaa gtcccgcctt cagatcagct ccttggtcga caatggcgag attaaaatct
      721 ccatccgaga gatgagtact ctttgggcgc ccatagataa ccctcatatc gaacgccgtt
      781 gtgtagaagt ttaccagacg ctcccgatcc ggacttacaa gtgctaaatg gcggatcctc
      841 ggcatgtgaa ttttcccttc cttcaattct gttcagtagc gaacttgatg tccgaactaa
      901 gttggccatc cgtgagttga aagatcaatg ggattgccgt cgggatcaag gaccctgtat
      961 tcggcctcac ggttccgcgg ccgcctctgg acttccgagc ctgcccccgc atcttttaac
     1021 ttctttttta gagcatcgag gtcttcgacc tcaaaaccaa aatgataaag gcccgcttct
     1081 cgtccggcat actgctcgtt taacgcgagg ttgattgaac cgtccgacaa gtaaatggcc
     1141 cctccttctc ccttcacctc cttcatccca aaagcttgct tataaaaagc cgctagattc
     1201 tcccggtttg agctcgcaat cgctatatga cggatcttcg gcatcgctca tctccttgca
     1261 cgtgattttt cgtccagatt aaaatcgtca ctctcaattg aaccagttgt caagtacgga
     1321 atcccatgct gattttcagc gcctaggcaa aggctctgct ccagcaataa gattgtaaag
     1381 cttaatgact tccggatccc tagggatagt cttgatcaat tcctccaact cctccggcat
     1441 gagaggggaa gcttctgcac gagtcatctt cttccagttg tcgtggaatt ccgggtcgtt
     1501 caatgatttc ctgaaggcat ccttcaaaat cttcacccga tcattcgggc tgtctggcgg
     1561 tagaacaaaa gcctggctga actgcatgaa ggcgataaac atgctaacga cgcttttttc
     1621 cttatctccc ttggccaaag tgtgcagtgc gggtaggtcc ttaaacgcgg ggtgactgaa
     1681 tcgatgacca cggggaaatt ccgccactcc atgaaagtgc atcatccctt gcttaatgaa
     1741 atcaggcgtg cgctgaacaa ctgtttccgc gctattggcg cgggcatcca attcaccctg
     1801 caagagtgcc gcgtccatct ctgctccaga gaaaccaact acaaatctgg ggtccttgag
     1861 atcaagaagc catgcaaaaa gccgggcaac gacatagttt gaatggccaa cggattgccc
     1921 tccgaccctc agcccttttg cgtttttcaa tttgtcgagc ttgtccaagc cggcttcttt
     1981 tctggtaaaa aaaacgtggg acccctcgct ttggccggaa cccaggtaaa tgaacttctg
     2041 aagatcgtac tgaactccgg gaagcccaag gatcgcaccc gtgacgagcc cgctaccaac
     2101 cctacctatg gtcaaaccgt cactggccgc catgctgtaa agatggttcg cggcttttct
     2161 accgccggcg cccggcataa actggactac tatcttcgga ttacccggga tgtgcttctg
     2221 cagaacagga atcaacgcct gggtgcgcag ctggccgacg ccacctggtg tgctgtcctg
     2281 aactaaagta atgagcttac cccgataaaa gtccggctga gcccacaaca ggttcggcgt
     2341 ccatccgaaa accatcgcaa gccaagccac agccatgcgt ttcattcggt acctccggct
     2401 atttgaaatc gagcgcgatc acttacctct tgtctgcatg gaaatatgag gaatatgggc
     2461 gcgctctaat tcctctctgc tgagtttttt aatgtccttt cggagctcgt aatttatgtg
     2521 ctggattgct tcggacggcc cggagcgatt gcccatcatt atcatcggcc cagtaccgct
     2581 gttttccagt tgatagaacg agcccccggt aatggtggca gccattcccg gcttcattac
     2641 ggccttaccg ccatcaggga aatgcatcgt gcattcaccg tcaatgacat aaaacgtttg
     2701 atccgcgttg tgacaatgca tgtcgtccct ctctccggga ttcggatagt agtggaccca
     2761 tgcgtggcaa cgctgcgtat taaacaccac cttacgacgc ttttcctgat gcgctagttc
     2821 aaccaagtca aagatttcaa cccccatgct aatcctcctt cctgctactg ataattgtcg
     2881 ctacttatct gtttaccgct tgtgcggctg agcttcacct gcgaaattca aaaccaccgc
     2941 ggccatgaat caagcttcgg gaattggctc cgaacgcgag ttctatcatc ctgtcttcga
     3001 aatgcgcgat cccccaaatt cacgattggc aaattttata atctattccg tactcatcga
     3061 tcagtatccg atcgcatagc cgtcgcgtct tggatccgca gctcccatga acacctgatt
     3121 ccgatgcaaa aaaaaccgat tccacaccgc cgaacgcgaa ttcccacggt ccacggagtt
     3181 tcacgtcata gccctgtcga agtaaaagct tgccgacgtc atctgaaaac cggctttcta
     3241 gctcaacttg acccggctgc tcgctttgcc aacgcggcgc ctcgatcgct tcttggatgt
     3301 tcatattgaa gtcgaaaaga ttgcatagtg tttgtgtgag gaattgagtt tggcccaaac
     3361 ctccaggcgt tcctatggcg agaatcggtc tacgttctct gagaaccaag gcagggctaa
     3421 gggtttgcgc gggaagcttt cccggcgcca gctcattgcg atgaccgggt attaggttaa
     3481 atccggacat gcggttgttc aaaacaattc cagtcccgtc cacgacaatg ccgcacccaa
     3541 agctttgatg cacgctttgt atgagcgagg cccagttgcc taactcatcc gccactgaaa
     3601 cgtacgtcgt gctacgtccg tgatcggcgc tcggaattgt gcccgttgcc gtgtaatcga
     3661 tggggaatct cttgagaaac tcgagagtcg cctcgtagtc atactcatcc ggatctccaa
     3721 tccacaactg gccggctctc catgcttcct ctttgatcct cacctgcaac gcaacgaact
     3781 cgggggtatt gtagccatag ttggatggat tgtactgcga cagacgtttg agctggagaa
     3841 gcaaaagcaa tccgaaggaa ttcggcggcg ggacgcatat ctcgtgtcct cgataggtcg
     3901 tggagagcgg ctcttcccat cgcgagcgat gtccgacaaa atcctgcagg ctgaaacacc
     3961 caccgtggta ttgtgaggct cggacgatag atctcgcgat ctcgccttcg taaaactcgt
     4021 ctcgtccgcc actggcgatg gcggctaaag tgatagcaag atccttttgc accaggaggt
     4081 ctcctacttc cggtgctttc ccctgcggga ggaagatgct cgaccaggcc ggatcggagc
     4141 caaatgagtt ttgagaggca cgaatcacat tagctaagtg aggaaagaca ggaaaaccgg
     4201 ttttcgcgta atgaatagcc ggctccagca aattggccat actgcggcta ccgagttttg
     4261 cagccgcttc cacccaagca tcgacagtcc ctggcgtgct tgcggccaaa attcctcgat
     4321 gtggaatgtt cttcgaaaaa agctcccgtc ggatttgtct tggagatcgt ccgctaccgt
     4381 ttatgacaag cgccctgctt tcattggctg aataaaagat tccgaacagg tcgccgccca
     4441 acccgcagtg atatgatttg acaacggaaa gcacggcaga tgcgcaaagc gccgcatcga
     4501 tcgcattgcc gccggccttc aacacgtcca gtgctgcgtc cgaagcgagc ggatgtcccg
     4561 tcgcaaccat tccgcgtgta cccattacca ccggcctatg actgttcata cgaagagatt
     4621 gcctagatca tcgtactggc gggtgatcga tgacagaact aaccaccatt ttgatgggat
     4681 atctaatcca gcttccgaac tatttacctc gaggccacca ggtttttctc tgatcgtcgg
     4741 tatcgagaaa tcaacggccg aaccatctaa atatgcagaa atgccccccg tgtggatact
     4801 cgggatctaa attcatccag ttacacaggc cacccgtgtt gcgagagatc aatcgggttt
     4861 ccatccgggt catgtactcg atactcggct tcacgattgg gcggtctctt ctggacttca
     4921 gttgccgctc caagctcttt gcacagcggt ttcaatgctt cgatgtcctc gacctcaatg
     4981 ccaaaatggt atagcccttc cttgtagtga ggccgttttt gaatgatcgc tacattgagg
     5041 tatccgtctg atagataaat agctggcccg acgccgtcga cgacctgcat tccaaacgca
     5101 gtcgtgtaga actttaccaa tctttcgcgg ttctccgtca caattgcgag atgtcgaata
     5161 cgtgccatag ttctcaagcc tctctttcct ctaatttcag ttttacttgt acaacttatc
     5221 gatgaaacct tcggcggcga gtttctgaac aggagcaaaa tacaggtggc gcttgatatc
     5281 catactggcc agctttggag caacgcgatg gcttatttca agagtgtttt tgatggcctt
     5341 gtccgatggt acgggcacac gatccaatcc ttgaacatac tgatcatagg ccgtttggac
     5401 cacccgcgga tcggaggttc tcatgtactt gcgcatgatc tcgattgatc tcccgcggtc
     5461 cttcacgagc gttgcaattc cctgtgtata ggcacgcaaa acattctgca cgatccgagg
     5521 attttcctcg gcaaatcttc ttgaagtcca aagcgcatca actatcagag gaataggatc
     5581 gattttcagg atctgtagac ctcgctcttc ggcgatcggt acgacggtat gagagatcgg
     5641 cgccgcatga accttgccgg tgctgagagc ggccaatcgc gacatcgtcc ctccaaccgc
     5701 cagatagttc acatccttgt cagggtcaag acccatgttg tgaatgacca tacgggcaat
     5761 ttcaccggct ctgccgccaa cttgcgatgt ggcgataatt ttacctttca gatcctggga
     5821 gccgcgcaca tctttggaga cagcgaaaac atacggcaac acgtttttgg ccgaagccag
     5881 taaaacgata tcggctccac cggcgatggc gctcaacgct gaaacggcgt cccctgacat
     5941 gaattgcgtc gtgttcgaaa gaaggctctg aatggacaag cggccacctt ccatataaac
     6001 gagctgagca tcgataccct gcgctttgaa gagtcccgct tctctagcca cccacaatgg
     6061 cgcagactct cccgttagcc ccggatagga catgtggagt tcctgcgcgg caacgacgcc
     6121 cagtcccgtt aaggacagaa acatcgacca aagaaaactg acaaatcgta gtttcatgat
     6181 cattctccgg gtacatagcg cttagaccat cctcatgagc tttccgaagc cggcaactgg
     6241 gctgcttatc ttcaaagttc gtagcgccat ccagatctct gaggtcaggt gtccaacggc
     6301 gggaattcgg aagtccgatt ctatggcctc gataacgtca agtgccggaa actgcgggca
     6361 cggaaggtaa agaccttcta gccggggtga ctgttgaacg gcgcgtttgg cggccgcgta
     6421 cacgtcgcct tccgaaacgt cgccgatacg ggtgaaatcc gctccgaggc tgaccaccgc
     6481 ctgaacttca aagccgtgag cggtcatgaa tgcgacgaca gcgtcattca attcaccggg
     6541 atagggattg ataagcccga cccgacgaac cgaaagcgcc ttgagcgctt
//
LOCUS       DASUTG010000524         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1562127, whole genome shotgun sequence.
ACCESSION   DASUTG010000524 DASUTG010000000
VERSION     DASUTG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562127"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VF089_12060"
     CDS             complement(<1..997)
                     /locus_tag="VF089_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6174741.1"
                     /translation="MTLILSNEDIQKALTVKESLAVMEESYREQAASRAVNRPTCHSY
                     LPHSLPNSTYSFKSVDGGVGKYGVLALRVTSDIVQEQTVNNTVRLEKIPLAGKGLFLG
                     LVQLFSTETGELLAIMPDGFIQQTRVGVTSALGMKFMARRDSKTLGLIGSGGQARAHY
                     RYMTAILPIEKVKVFSPNAEHCRAFAQQMSKETGIAGEPVNSPEEAAKGCDVICSATN
                     SSRPVVKVEWLEPGMHYNSIREFETDIGALEKCDVVAIHTQFGGIYHYQPPGIVEDMP
                     GVRREKQRDWSRYPEICDIIAGKVTGRNNDQQITFFLNNVGTGVQFAAMGYCAFKA"
     gene            1666..2751
                     /locus_tag="VF089_12065"
     CDS             1666..2751
                     /locus_tag="VF089_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6174742.1"
                     /translation="MTLILNNDEIETVFTLEECFDVLEPALRDLGNGSAVSMPRHDLL
                     VPGPLDGSYHGLKTSCASLPRSGVTTMRLTSDILTWPIVSGRQRRVKVPLAVGSKYVG
                     LVFVFSTATGELLAILPDGFIQAIRVGVTNALSAKYMARPDSSVLAVYGSGWQARPAV
                     LAMCKVCPIGEIRVFSPTKSNCERFAAEMRQLVHAKIVAISSAEEAAKNADIVSLATN
                     ALDPFFPATWLQPGMHLTTVRPSEMMLEALLRCDRIAVSTREAAQLFTLPGQESAVPE
                     FGKGDYGRKELEKTAADWRDKAELSEIIAGKVKGRDKDSDVTCMLNHLGLGLQFSAAA
                     ARVYELAREKGLGRELPTEWFSQDEHS"
     gene            complement(2934..3653)
                     /locus_tag="VF089_12070"
     CDS             complement(2934..3653)
                     /locus_tag="VF089_12070"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174743.1"
                     /translation="MYGWRAKIGRISPSPETVGAEEWRRSLPCGVCLVETRTLLHDVT
                     VDGLSETAKQVERAALELASAEVDVILQAGTAIAFFRGFGHDHELSRRIHAVTGIQAT
                     TSLTAVVEALRSLEIQRPAIATSYLAEIDARLVGVLEKSGFEVAAIRGMGLKKSIEMG
                     KVTPEETYRLAREVARSAPNADGILISCGNLRTFEAIEPLEIDTGLPVVTSNQAGLWQ
                     VLRMTGIKDRLTSLGRLLREN"
     gene            complement(3661..>4064)
                     /locus_tag="VF089_12075"
     CDS             complement(3661..>4064)
                     /locus_tag="VF089_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6174744.1"
                     /translation="AEQDFAVEVRKLTDGKGVDAVYDSVGKATFEGSLDCLAPLGSLI
                     IFGQASGPVPPFDTSILNAKGSLTLARPSLTHNVTSHADVLWRAGDLFNWLAEGNLTV
                     KIGEAYRLHQAAEAHRQLESRKSSGKILLVP"
BASE COUNT          875 a         1109 c         1112 g          968 t
ORIGIN      
        1 ccgccttgaa tgcgcaatag cccatggcgg caaattgtac cccggtgccg acgttattca
       61 aaaagaatgt gatctgctgg tcgttgtttc ggcctgtgac tttcccggcg atgatgtcgc
      121 agatttcagg gtagcgcgac cagtcgcgct gcttctcccg ccgcacccct ggcatgtctt
      181 caacgatgcc gggcggctgg taatgataaa tgccgccgaa ttgtgtgtgg atggcgacca
      241 cgtcgcattt ttccaacgcg ccaatgtctg tctcaaattc acgaatcgag ttgtagtgca
      301 tgccgggctc cagccattcg acttttacca ctggtcgaga ggaattggtc gcactgcata
      361 tgacgtcgca gcctttcgcc gcttcttcag ggctgttaac cggttcgccc gcaatcccgg
      421 tttctttgga catttgctga gcgaacgctc tacagtgctc ggcgttcggg ctaaaaactt
      481 tcaccttttc aattggcagt atggccgtca tataacggta atgcgcgcgc gcctgcccgc
      541 cggatccgat taacccgaga gttttagaat ctcggcgtgc catgaatttc attcccagtg
      601 cgctggtgac gccgacgcgg gtctgttgaa tgaatccgtc cggcatgatc gctaaaagct
      661 cacccgtttc ggtgctgaag agttgtacca ggccgagaaa caaaccttta ccggcgagtg
      721 gaattttttc caatcgaacc gtgttgttga cggtttgttc ctggacgatg tcggaggtta
      781 ctcgtagagc tagaacacca tacttaccga caccgccgtc gacggattta aagctgtaag
      841 tcgaattggg cagcgagtgg ggcaaatagg aatggcaagt ggggcgattg acggcgcggc
      901 tggcagcctg ttcacgataa gactcttcca tcacggcaag gctttccttg acggtcaagg
      961 ctttctgaat gtcttcattg ctgagaatca gggtcacgct ttttcctccg cgcctcgtta
     1021 ttttaacgtt atgacttaac cgatccgact cgggttgtcc aatcattgcc ttttgcgatg
     1081 ctgacttcgc tgccagcgag tggtttgatc ctgcgcaagt ccgcgcgtat ggtactggca
     1141 tgtcctttag gatttccgtt caggtaaaaa cgcaagctcg gaaagaaaac atcgtcaaaa
     1201 tctctcagca ggaatatcaa atctctgtac aggctgcacc cgttgcagga aaggccaacc
     1261 aagcactggt cgacctgctg gcgaaatatt tctcggtccc aaaatcctct gtaaagctgc
     1321 ttcacggtca atcttcgcca agaaagctat tcgagatcgg ttaaccttga cttgcgtcgc
     1381 ggcgcgaaat cggcttattc ggagtgttga aagtattaag gcaaggtggc gaaagcgaaa
     1441 ctgtggcatg gtaaacagcc ccaaaaagaa ttgcgagcag acaagctcgt gtacgaagga
     1501 tttggcgttg gtcacaagcg cgagcgctcc aaccagcagt cggaatggag actgtttgcg
     1561 aaaattccga actttcacag caatcttcgc cgcggtcctg cctcatgtgg atgctgctca
     1621 ggccagggtc tgttaagtca ctcgtcaaaa ttgtcgggag tttgaatgac tctcattttg
     1681 aacaatgatg agatcgaaac cgtgttcacc ctcgaggagt gcttcgatgt cttggaaccg
     1741 gcgctccgcg atcttggtaa tggcagtgcg gtcagtatgc cccggcacga tctcttggta
     1801 ccgggaccgc tcgacggtag ctaccacggc ctgaagacat cctgcgcttc gttgccgcga
     1861 tcaggggtga ccaccatgcg gctcacctcc gatattctta cctggccgat tgttagcggc
     1921 cggcagcgta gggtcaaggt tcctttagcg gtgggctcca agtatgtcgg cctggtgttt
     1981 gtctttagca ccgctactgg agaacttttg gcgatccttc cggacggctt cattcaggcg
     2041 attcgcgtcg gcgttaccaa tgcgctctct gcgaaataca tggcacgccc cgactcttcg
     2101 gtgctggcgg tgtatggctc gggttggcag gcacgcccgg cagtgctcgc tatgtgcaag
     2161 gtttgcccta tcggcgagat ccgggtgttt agtccaacca aaagcaactg cgagaggttc
     2221 gccgccgaga tgcggcaact ggttcacgca aaaatcgtcg cgatttcgtc agcggaagaa
     2281 gccgccaaaa atgccgatat cgtttccctt gcaaccaatg cgctcgatcc ttttttcccg
     2341 gctacatggc ttcagccggg tatgcatcta accacggtac gacccagcga gatgatgctt
     2401 gaagccttat tgcgctgcga tcgtatcgct gtttcgaccc gcgaagctgc tcagctcttc
     2461 acgttgccag gtcaggagag cgcagttccg gaattcggca aaggtgatta cggccgcaag
     2521 gagctggaga aaaccgctgc ggactggcgc gacaaagcag agctctcaga aataattgcg
     2581 ggcaaagtca aaggccggga taaggactcc gatgtcactt gcatgctcaa tcatttggga
     2641 ttggggcttc agttttccgc cgcggctgcg agagtgtacg agcttgcgag agagaagggc
     2701 ttgggaagag agttgccaac ggagtggttc tcccaggatg agcactcgtg agcgtccaga
     2761 ctgatgaaaa aggtctatct gcttcgttgc cctcgcccga ctcgctacaa cgtcctggta
     2821 cgtacgtctt cgctcgtcga tcttcgcgcg cctcgcatct aaaccttttt gatcagtctg
     2881 tatgagccct actacactgc tgaagaaacc tcttccaaca acgaagaccc ccatcagttt
     2941 tctctaagaa gccggcccag agaggttaac cgatctttta ttcccgtcat tcgtagcact
     3001 tgccatagtc ctgcctgatt acttgttacc accggcaagc cggtatcgat ttccagcggc
     3061 tcaattgctt caaacgtccg gagattaccg cacgagatta gaattccatc ggcgttcggt
     3121 gctgaacgtg cgacctctcg agccaggcgg taggtttcct cgggtgtcac cttgcccatc
     3181 tcgatactct tcttgaggcc catgccgcga attgctgcca cttcaaagcc ggacttctcc
     3241 aaaactccaa cgagtcgagc atcgatttcc gcaagatacg acgttgcgat cgccgggcgc
     3301 tgaatctcca gggaccgcag cgcctcgacc accgcggtaa gcgatgttgt ggcttggatg
     3361 ccggttacgg catggattcg ccggcttaat tcatgatcgt ggccgaagcc gcgaaagaac
     3421 gcgatggcgg tgccggcttg gagaataacg tctacttcgg cggaagctag ctccaaggcg
     3481 gcgcgctcca cctgcttggc ggtttcgctg aggccatcca cggtaacatc gtgaagaagt
     3541 gtgcgtgttt caacaaggca gacaccgcat ggcagagagc ggcgccattc ctcggcgccg
     3601 accgtttcag gagaagggga gatacgcccg atctttgcgc gccaaccgta cataaatgat
     3661 ctaaggaact aggagaatct tgccgctgga tttgcgtgac tctaactgcc gatgcgcctc
     3721 tgcggcttga tgaagcctgt aggcttctcc aatcttaacc gtaaggtttc cttccgcaag
     3781 ccagttaaat agatcgccgg cgcgccagag cacgtcggca tggctggtga cgttgtgtgt
     3841 cagacttggg cgggccaagg tcagcgaccc cttcgcgttt agaatcgaag tatcaaacgg
     3901 tggcaccggg ccgctggctt ggccgaaaat gatcagactg cccaaggggg cgaggcagtc
     3961 aagacttcct tcaaatgtcg ccttaccaac tgagtcatag accgcatcaa cgcctttgcc
     4021 gtcggtcaac tttctaacct cgacggcaaa atcctgttcc gcat
//
LOCUS       DASUTG010000525         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_971476, whole genome shotgun sequence.
ACCESSION   DASUTG010000525 DASUTG010000000
VERSION     DASUTG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971476"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..643
                     /locus_tag="VF089_12080"
     CDS             17..643
                     /locus_tag="VF089_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174745.1"
                     /translation="MAIGINSPGRLIDRLAKDFVKRFNLANDVRIRPVGGQGQLEPYN
                     AMKADIVQAALVTPPLEVRGQRDGFNVIFDFRTLDASSIYSALQASTKVVRERPGAVQ
                     RFVAGMAEAVHFVENNPDRAKQSVSKILRLDDEVALGSAYQAYARSIVNRSLLIPGNA
                     VAEGLENARTIGVNVKKKPEELFDNTFAHDLEKSGFLKELWGTGVKAK"
     gene            complement(798..1097)
                     /locus_tag="VF089_12085"
     CDS             complement(798..1097)
                     /locus_tag="VF089_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX6174746.1"
                     /translation="MTAHGEALRAESQDDQLVDGVKQDYTRLNLSPGERAMLDYAVKL
                     TVAPSSVGEEDIQTLRAQGFDDRDILDIVYVICLYNFNDRMADAMGIKGHDFLHG"
     gene            complement(1098..1352)
                     /locus_tag="VF089_12090"
     CDS             complement(1098..1352)
                     /locus_tag="VF089_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6174747.1"
                     /translation="MPYIKTIPPAEANGKLKEIYQSGAGPSAARGKVSMIRQVQSLNP
                     DVLAAWRALDVGIMQGESRITRRQREMIATVVSATNRCSY"
     gene            complement(1355..1564)
                     /locus_tag="VF089_12095"
     CDS             complement(1355..1564)
                     /locus_tag="VF089_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174748.1"
                     /translation="MEKLYVNEIRPDGEVSTVGLFYSRAEAEKIVAMLRDIPERAACR
                     YEIVQAVRHLLAEKSTARAQTAPEE"
     gene            complement(1570..>2228)
                     /locus_tag="VF089_12100"
     CDS             complement(1570..>2228)
                     /locus_tag="VF089_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6174749.1"
                     /translation="DMVGVCRLQPHMIDLGSEVAHEFVIACCVAEDYEKVNQGPDAVE
                     EEAMRTYARCTEIATALASYVRDMGYPAIAHHNGASEVQAIPIFYQVGFGELGRHGSL
                     INQEYGASFRPGFVTTDLPLVEDQPREFGVQDFCMNCNVCQRNCPGDAIPQDHVMTHG
                     IKRWLIDLEKCYPYSRLRDEYCHLCVDVCPYIVKSHPETYKAFMKERKQVGYKTPKTY
                     "
BASE COUNT          480 a          645 c          623 g          480 t
ORIGIN      
        1 cgaggacttg aaaggcttgg cgatcggcat caactctccg ggccggctca tcgaccgact
       61 agccaaggat ttcgtcaaac ggttcaacct cgccaacgac gtgagaatcc gcccggtcgg
      121 tggtcagggc cagttggagc cctacaatgc aatgaaggcc gacatcgttc aggcggcgct
      181 ggtcacgccg cctttggaag ttcgcggcca gcgcgacggc ttcaacgtga ttttcgattt
      241 tcgcacactc gatgcatcgt caatctattc cgctctgcaa gcttcgacga aggtcgtgcg
      301 cgagcgaccg ggcgcggtgc agcgtttcgt cgccggcatg gcggaggcgg tgcacttcgt
      361 cgagaacaat cccgaccggg caaagcagtc ggtcagcaaa attttgcggc tagacgacga
      421 ggtcgcgctt gggtccgcct accaggccta cgcccgctcg atcgtcaatc gctcgctctt
      481 gattcccggc aatgccgttg cggagggctt ggaaaacgcc cggaccatcg gcgttaacgt
      541 caagaagaag ccggaagaac tattcgacaa cacatttgcc cacgatttag aaaaaagcgg
      601 gtttttgaag gagctctggg gcaccggcgt caaggccaaa taaagcatta gcctggtcgg
      661 cgctcgatac gtccaaacta aactcgagaa ctgacgattc tagaacaagg atcgcaccgc
      721 agggcaggcg agagatttca tatatccacg agaattctct ctagaatcgc gaagatctcg
      781 tcgaagcagg aacggtatca gccatgcaga aaatcatgtc ctttgatgcc catggcatcg
      841 gccatccgat cgttgaaatt gtaaagacag atcacgtaga cgatatccaa aatatcgcga
      901 tcgtcgaaac cctgcgcgcg cagggtctga atatcctcct cgccgacgct cgagggcgcg
      961 acggtcagct tcactgcata gtccaacatc gcgcgttcgc ccggcgataa attcaggcgg
     1021 gtatagtcct gcttcacccc gtccaccagc tgatcatctt gggactcggc acggagagcc
     1081 tctccgtggg cggtcattca atagctacat cgattcgtcg ccgatacgac ggttgcaatc
     1141 atttcccgct ggcgccgggt gatgcgcgat tcgccttgca tgatgccgac atcgagcgcg
     1201 cgccaggcag cgagaacgtc cggattcagg ctttgcacct ggcgaatcat ggaaaccttg
     1261 ccgcgcgctg cggaagggcc ggcgccggat tgataaatct ctttgagctt gccgttcgcc
     1321 tcggcaggcg gaattgtttt gatatagggc atcgtcactc ctctggcgca gtttgagcgc
     1381 gtgccgtgct tttctcagcc aacaaatggc gcacggcttg aacgatttcg taacggcagg
     1441 cggcacgctc gggaatgtcg cgcagcatgg cgacgatctt ctcagcttcc gctcgcgaat
     1501 agaacagccc gacggtgctg acttcaccat ccggacggat ctcgttgacg tacagttttt
     1561 ccataagctc tagtaagtct taggcgtttt gtagccgact tgcttgcgct ctttcatgaa
     1621 tgctttatag gtctccggat gcgatttcac gatgtatgga cagacatcga cgcatagatg
     1681 acagtattcg tcgcgcaggc gcgagtatgg atagcatttc tccagatcga tcagccatcg
     1741 tttgatcccg tgtgtcatca cgtgatcctg cgggatcgca tcgcccggac aattgcgctg
     1801 gcacacgttg cagttcatgc agaaatcttg aactccgaac tcgcgcggtt gatcttccac
     1861 caatggcaaa tctgttgtga cgaacccagg ccgaaagctt gcgccgtact cctggttgat
     1921 caaactgccg tggcgcccga gctcgccaaa accgacctgg tagaaaatcg gaatcgcctg
     1981 cacctcgctg gcgccgttgt ggtgagcgat ggctggatat cccatgtcgc gtacatagga
     2041 agccagcgcc gtcgcgatct cggtgcacct ggcgtacgtg cgcatcgctt cttcctccac
     2101 ggcgtcaggt ccctggttaa ctttttcata atcctcggcg acgcagcaag cgatcacgaa
     2161 ctcatgcgcc acctcggagc cgagatcgat catatggggc tgcaaccggc agacgccgac
     2221 catgtcgg
//
LOCUS       DASUTG010000526         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_525391, whole genome shotgun sequence.
ACCESSION   DASUTG010000526 DASUTG010000000
VERSION     DASUTG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525391"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..614
                     /locus_tag="VF089_12105"
     CDS             390..614
                     /locus_tag="VF089_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174750.1"
                     /translation="MKALILLLLLGNLGLAGCEFVAGAATGALATGAGYEINAKRQMD
                     RLDDDYRRERIGRREYERRRSQIERGSIVY"
     gene            1059..2561
                     /locus_tag="VF089_12110"
     CDS             1059..2561
                     /locus_tag="VF089_12110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6174751.1"
                     /translation="MCYRHLDIFRTMFKAVSLLLVGVLFFGCTSDIIPATGERRYLGY
                     TWQQETELGKEASKQVAALFGVYRDPKLERYVTEVGNRVLATSHLRRPGAEEQFQKTP
                     VTFTVLDSPIINAMALPGGYIYVTRGMLSHLNNEDELAAVLGHEIGHVTARHAARQAW
                     QQQLGQGLLLGGAILGQGLGLPAQEILNVGGMAAQLLFLRYSREDELESDQLSVEYSS
                     LAGYDPAEVISFFQTLNRIQEKEGQGMPNFLSTHPNPGDRVQRIKELTDRRRIEPPPQ
                     PTGERYLNAIDGLVIGEDPRQGFVEGNIFYHPDLRFRFPVPRGFKVVNQPTQVVMVES
                     QNRAILGFTSGPEKTVESAAAKFINQPGLRVIQRTPMRSNGLPAMLVIADGQMEKGQV
                     VRLMVYFIEYRGSVYHFLGYSAPQAFSMFQKEFMQTMQGFGELQDSRIANKEAVRLRL
                     ERVSRQARFQDLIPRNLPAPFTPEDVAIMNQVQLNDEIRPGRTIKLPRMR"
     gene            complement(2717..2881)
                     /locus_tag="VF089_12115"
     CDS             complement(2717..2881)
                     /locus_tag="VF089_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEX6174752.1"
                     /translation="MQSLKKRVSLLAITISLVGILGSIGCNTVKGMGRDIERGGEKTQ
                     DAAESVRRRL"
BASE COUNT          693 a          794 c          814 g          683 t
ORIGIN      
        1 gcgcctgagc atagcggtga ctcttgcctt aggagtgagc tgtcggcggg ttaataatcg
       61 atagatgatg agtcccgcga ttagagaaat gagccagaac atcggattgt ccattctgac
      121 ctcctcgctg atgaaaaaca cgcaatgccc cgccgcgtca agtcgtagaa aaataatcaa
      181 agtggctatc actggctatc aagcacgcct ctggcaatag aggtaaaatt ccggcgagtc
      241 gtagtacgtc catcctgcct tgccggttta accctctcct gctctgtgta atcgtttaat
      301 cctctctatg tgctgcccca gccggagctc gttcaccggt ttattaacag aagccgagaa
      361 aatgagtcta agcagatcag gagggaatca tgaaagcctt gatcttactc ttgcttctag
      421 gaaatctagg cctggcgggc tgcgagttcg tcgccggagc cgctaccggt gctctggcca
      481 cgggagcggg atatgaaatc aatgccaagc gacaaatgga ccggctcgat gatgactacc
      541 gccgcgagcg cataggccgc cgcgaatatg aacgccgcag aagtcagatc gaaagaggct
      601 ccattgtata ctgagtgcgg cgatcaattg tctacggccg gtaatcgccc cggccgggag
      661 actagattgc gattgcaccg gagtacacgg tcatagctgt ttgatcatcg tctaggagcg
      721 aacccgaatt ctgctcctgt gatggaagcc gcacccggga gtgggcttga agttacagag
      781 gacaattccg actttgatag gttctcgatt catcgacatg agcggcgatc aacttttcgc
      841 tcctgccaac tgctttgatt aggtctgcgg ttactgcggc attgtcaaag ctgccagttc
      901 tcccgcctcg ttctctctaa aaatgtgccg ttctattcca tcgccgcgcc gacgtgtata
      961 ttaaaagggg gccaccctgc gtgcgcccgc aaattcacag gcgcggcgat ctctgctgct
     1021 tgagctggga accagcgcaa gcgaaaagga ggaggaggat gtgctacaga catttggata
     1081 tttttcggac catgttcaaa gcagtgtccc ttctgctggt tggtgttctt tttttcggct
     1141 gcacatcgga cattattccc gcaaccggtg aaaggcgcta cctgggttat acttggcaac
     1201 aggaaaccga gcttggcaag gaagccagca agcaagtcgc cgcgttgttt ggtgtgtatc
     1261 gggatcctaa actcgagcgc tacgtcaccg aggtcggcaa ccgggttctc gcaaccagcc
     1321 atctgcgccg gccgggagcc gaggagcagt ttcaaaaaac accggttact ttcaccgtgc
     1381 tcgacagccc gatcatcaat gccatggcgc tgcccggtgg atatatctat gtcactcggg
     1441 ggatgctcag tcatttgaat aatgaggatg agctcgcggc cgtgttggga cacgagatcg
     1501 gccatgtcac cgcgcgccac gcagcgcgcc aggcatggca acaacagttg gggcaaggac
     1561 tgctgctcgg tggcgccatc ttaggccagg ggcttggcct tccggcgcag gaaattctca
     1621 atgtcggcgg catggccgcg cagctactgt ttcttcgcta cagccgtgag gacgagctgg
     1681 agtcggatca gttgagtgtc gagtacagct cacttgccgg ctatgacccg gcggaagtga
     1741 tctcgttttt tcaaacgcta aatcgcatcc aggaaaagga aggacaaggg atgcccaact
     1801 tcctttcgac gcatcccaac ccaggcgatc gggtgcaacg catcaaagaa ttgaccgatc
     1861 gccggcgtat cgaaccgccg ccgcagccga caggcgagcg ttacttaaat gccattgacg
     1921 gcttggtaat tggcgaagac ccgcggcagg gtttcgtaga aggcaatatc ttttaccatc
     1981 cagatctgcg cttccgcttt ccggtgccgc gcggctttaa agtggtcaac caaccgactc
     2041 aggtggtgat ggtcgagagt cagaaccgag cgatcctagg ttttacttcg ggccccgaga
     2101 aaactgtaga atctgccgct gcaaaattca ttaatcagcc cggcttgcgg gtcatccaac
     2161 gcaccccgat gcgctccaac ggcttgcccg caatgttggt catcgccgac ggacagatgg
     2221 aaaaaggtca ggtcgttagg ctgatggttt acttcattga atatcgtggc tcggtttacc
     2281 actttctcgg ctacagcgcg ccccaggcct tcagcatgtt tcagaaggaa tttatgcaaa
     2341 ccatgcaggg attcggtgaa cttcaagatt cgcgaattgc gaacaaagaa gccgtccgcc
     2401 tccggctcga gcgtgtttcg cgacaggcgc gctttcagga tctgattcca aggaacttgc
     2461 ccgcgccgtt tacgcctgaa gatgtcgcaa tcatgaacca ggttcagttg aatgacgaaa
     2521 tccgaccggg aagaacgatt aagctaccgc ggatgcgtta atagcaggta gagtttatgc
     2581 ttcttcgtac gatgcggatt cgaaatgtag catctatgag acagaggagc gaaagagagc
     2641 cccaaaagta aagcggagga gctacataat ctagctcctc cgctcttcta ctcggtgaaa
     2701 tgttatttgg cttcgcttag agcctgcgtc ttactgattc cgccgcatct tgtgtctttt
     2761 cgccgccacg ctcaatatcg cgtcccatgc cctttacggt gttgcaaccg atagagccca
     2821 agataccaac cagcgaaatc gtgatcgcta gcagggaaac acgttttttc agtgattgca
     2881 tattaacctc ctatgttgtg tagtccgcta actgacttga gcaaagctcg taccaaaagg
     2941 agtcgagtct cgccgtctca ttgaggcagc gctcgtacag caaa
//
LOCUS       DASUTG010000527         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1861756, whole genome shotgun sequence.
ACCESSION   DASUTG010000527 DASUTG010000000
VERSION     DASUTG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861756"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF089_12120"
     CDS             complement(<1..501)
                     /locus_tag="VF089_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX6174753.1"
                     /translation="MNRASQDFEVIVASLKRRHTGVTSTVAALIPEQAKRLDIAALGP
                     KVPAQWPRITWSDLFRRGFNPPPGKKFRIWHARRNLEALTGLILRSVLGMPLKLVLTS
                     AAQRRHSRWTHWLMRQMDAVVATSAETANYIPVPVKIISHGVDTGRYFPADDRSREWS
                     GTGLPGR"
     gene            complement(579..1574)
                     /locus_tag="VF089_12125"
     CDS             complement(579..1574)
                     /locus_tag="VF089_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174754.1"
                     /translation="MKLRIFSTLVVLLILTFSPAPAIAQKIRAASGGLSVIHSLLWVA
                     YDQKLLKKYGMDMEYIAIENGTVGMQTLVANESQFLFSTGSLAVNANLRGADITIVAG
                     GINFIPDKLIVRPEIKTAEDFKGKRIAISRFGSSSEVNLRIALEKIGVKADQVQILQL
                     GGVSTRQAALLSGQVQATVLSDPQGTSAIKAGMNLMVDLSDKKWGLPRYCHNCFMTTR
                     KFLETHREQAVGFIKAMIEGLYMMKRDKNLAVRLIKKYLRLDDESASIGYDFYIAQHG
                     DGILSLPEPRGLEFVIAEVAKQNAKAAAATPDSLRLLDPTILDEIKKSGFIERVK"
     gene            complement(1677..>2414)
                     /locus_tag="VF089_12130"
     CDS             complement(1677..>2414)
                     /locus_tag="VF089_12130"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6174755.1"
                     /translation="TSMLLNAQESILILPSQTRYEQRGGGTSTSTERRIRFSPEIPGH
                     RIGEALPEWEIPMRIGRKAMPNGELLFPFDDTQSIREEMARVMPIYHGIEKLTKEGDH
                     IQWGGPFLFKHGFTSMPENRALFTPMKPPDRRPAPGKFYLSTRRGKQFNSMTFGHTHL
                     SMGTTSRHVIFMSPDDAERLELEDGYEVILRSDTGRMKGIVQLAPLKSGTFQAYWPEA
                     NVLIPRRIDPVSGGPDYNVEVWIEKDQ"
BASE COUNT          506 a          643 c          676 g          589 t
ORIGIN      
        1 acgcccggga agtccggtcc cgctccattc ccggctgcgg tcatcagctg gaaagtagcg
       61 tcccgtgtcg acgccgtggg agatgatctt gaccggtacc gggatataat tcgctgtttc
      121 agctgaagta gcgacgacgg catccatttg ccgcatcagc caatgcgtcc aacgactgtg
      181 acggcgctgc gccgccgatg tgagaacgag tttaaggggc atgcccagta cggaacggag
      241 aatcagccct gtgagcgcct caaggttgcg gcgcgcatgc caaatgcgga attttttccc
      301 aggtggtgga ttaaatcccc gcctgaagag atcgctccag gtgatgcgcg gccattgcgc
      361 cgggaccttg ggcccgagag cggcgatatc taatcgcttg gcctgttctg gaatcagagc
      421 ggcgaccgtt gatgtcaccc cggtgtgccg ccgctttaga ctcgcgacga tgacttcgaa
      481 atcctgcgat gcgcgattca tagataaatc ttgggacacg aagagggctc agagaagaga
      541 tcgcttgttc ggttcgtttc tcctctaagt tcgagccttc atttcacccg ttctataaat
      601 ccgctctttt tgatctcatc aaggattgtc ggatcgagca gtcgcaggct gtcaggggtc
      661 gctgccgcag ctttagcgtt ttgtttggca acctcggcga tgacaaactc gagacctctt
      721 ggttccggta agctcagtat tccgtcgcca tgctgggcga tgtagaaatc atatcctatg
      781 gatgcgctct catcgtctag gcgcagatat tttttgatta gccggaccgc cagattctta
      841 tcccgcttca tcatgtagag cccttcgatc attgctttaa tgaaaccgac cgcttgctct
      901 cggtgcgttt cgagaaactt cctagttgtc ataaaacaat tatggcagta gcgcggcagt
      961 ccccattttt tgtccgacag atcgaccatc agattcatgc ctgctttgat ggccgaggtc
     1021 ccctgaggat cgctcagcac cgtcgcctgg acctgaccgg agagcaaagc ggcctgccgt
     1081 gtggatacgc cgcctagctg gaggatttga acctggtcgg ccttcacgcc gattttttcc
     1141 aaggcgattc tgaggttgac ctctgaggag gagccgaacc ggctgatggc gatacgtttg
     1201 cctttaaaat cctcagcggt tttgatttcg ggtcggacga tcaatttatc gggaatgaag
     1261 ttaatccctc cggcaacgat cgtgatgtcg gccccccgta agtttgcatt aactgccaag
     1321 gatccggtgg aaaagaggaa ctgagattcg ttggcaacta gagtctgcat gcccaccgtg
     1381 ccgttttcga tcgcgatgta ctccatatcc atgccatatt tcttcaacag tttttgatca
     1441 taagccaccc agagcagcga atgaattaca gataggccgc cgctggccgc gcgaattttt
     1501 tgcgcgatag cgggcgcagg gctaaacgtg aggattagga gaacgacaag tgttgaaaag
     1561 attcggagct tcacattttt ctcattcctt gtgatcaact ggcgatggcc aacacaaatg
     1621 ccgacaaacg caaccttctg gatgcaaccg gcgatagggt gaatcatcgg gcgccactac
     1681 tggtccttct caatccaaac ctcgacgttg taatccggcc cgccagatac aggatcaatc
     1741 cggcgcggga tcaagacatt ggcttcgggc caatatgctt gaaaggttcc tgatttaagc
     1801 ggcgcgagct gaacgatccc tttcattctt ccggtgtccg agcggaggat cacttcatag
     1861 ccgtcttcca gctctagcct ctcggcatcg tcgggcgaca taaagatgac gtggcgcgat
     1921 gttgttccca tagaaagatg ggtgtggccg aaggtcatgc tgttgaactg tttgccgcgc
     1981 cgcgtcgaga gatagaattt acccggggcg gggcgtcgat ccggtggctt catcggggtg
     2041 aatagggcgc ggttctcagg catgctggtg aagccatgtt tgaaaagaaa cgggccgccc
     2101 cattggatgt gatcgccctc cttggtgagc ttctcaatgc cgtgataaat cggcatcacc
     2161 cgggccattt cttcgcggat actttgcgtg tcgtcgaagg ggaaaagaag ctcgccattg
     2221 ggcatcgcct tgcgcccgat ccgcatcggt atttcccact caggcagcgc ttcgccgatg
     2281 cgatgcccgg gaatctccgg gctgaaacgg attcgtcttt cggtgctggt cgatgttccg
     2341 ccgccacgtt gttcatagcg cgtttgactc ggcaggatca gtatcgattc ctgagcgttg
     2401 agcaacatgg aagt
//
LOCUS       DASUTG010000528         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1711932, whole genome shotgun sequence.
ACCESSION   DASUTG010000528 DASUTG010000000
VERSION     DASUTG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711932"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF089_12135"
     CDS             complement(<1..394)
                     /locus_tag="VF089_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HEX6174756.1"
                     /translation="MPTRTIEIAGRPITLDARPDRLDLRDLPYRPSVVCLAPIYPGEK
                     DISKLLPRYLAAGLVLDQESEGACTGFGLAAVINYLLWRRSGLKLQSKDKVSPRMLYH
                     LARFYDEWPGEGYEGSSCRGALKGWHRHG"
     gene            complement(621..1436)
                     /locus_tag="VF089_12140"
     CDS             complement(621..1436)
                     /locus_tag="VF089_12140"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX6174757.1"
                     /translation="MAKYAVCIGINDYPGTGSDLAGCVNDVRDWSALLQKKGFAVLQI
                     VDRKATGKNIRSAIESTIGGAKSGDTVIIQYSGHGSYVPDKNGDEPDGTDECICPYDI
                     KSKGPITDDELFELYSSKKAGVKLLVISDSCHSGTVAKFAPITTPPTIKGKGAPIRKV
                     RFLPPATFLPKRDIGKLGIGRGVRRSNPPGRYAALLMAGCQDTEYSYDTSFEGRPNGA
                     FTFVAIRALSGLSPEATYKQWYQTIRKALPSQQYPQSPNLYGTSRMKAWKALA"
     gene            1698..2753
                     /locus_tag="VF089_12145"
     CDS             1698..2753
                     /locus_tag="VF089_12145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174758.1"
                     /translation="MNGLRSSVLTSLAGVLLAGLLSAAPALAQSDFYKGKTITVYIGT
                     TAGALYDQWGRILATHMVKHIPGNPNVIVQNMPGAGHMIAANYVYKTTKPDGLSLIGS
                     IVPTLYFDQLVGRKEAQYDWAKFVWIGSPVKGELQMYMRADTPYKTMDDVRNAKEPPR
                     CGAQGTSDTAYYVPKLFEEVIGTKFNLVQGYPGGPEIDLAVERGEIHCRAFTIEAFFS
                     REPYHTWRKKGFVRNIIQTGKKRDSKLPDTETIWELMDKYKTPEADRRLATVMLASGA
                     LGRPMLATPGVPADRVKILREAFNKTMEDKEFLAELEKRKFELDPTPGEELEKLVKDV
                     MSQPPEIVARIKKLLGQ"
     gene            complement(2823..3818)
                     /locus_tag="VF089_12150"
     CDS             complement(2823..3818)
                     /locus_tag="VF089_12150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174759.1"
                     /translation="MISRTGKANKLFLAAAFLCLYFPAQSIFGADKIRIAYTSPGPQH
                     GVLWVGDVAGVFKRNNLDLEIIYMPGNISMGSLISGELNFGQMTGALMSPARLQGSDV
                     VMLVSIQELLDDRLVVRPNIKTPEELKGKRIAVSRFGAASHMRVLNMLPRFGLTDKDI
                     TFLQIGDTPARIIALLGNSADASSFSPPDHLAAVHAGMRVLLNMAELNIYYQGTGLVA
                     SQRYIAKNRDVTRRMVKSYVDAIQIVRTNPELAKRAFVKYRKTKDEKQLEDAYQTLLE
                     IVKPKPYPNLESFKTIFKDVSERLPAARTANAKEFVDMSFLEELDKSGYIDGLYR"
     gene            complement(4128..4613)
                     /locus_tag="VF089_12155"
     CDS             complement(4128..4613)
                     /locus_tag="VF089_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174760.1"
                     /translation="MADRILVALDETQRIHRFMAHLRMTAKPGDTIIFLIRTATSTWG
                     NVETHLPMIETGSITSREYRGSAWRFNVDLQKVKAEQNLASMCEALRRNGINPEVKMY
                     AGGLSKAVRESTKAGATRLIVMPPNHTILYILKALLAALRLGHHVPPVALLPPHLCKV
                     A"
BASE COUNT         1055 a         1249 c         1332 g         1091 t
ORIGIN      
        1 cgccgtggcg gtgccagcct ttgagtgctc cccggcagct cgacccttcg tagccttcgc
       61 caggccattc atcatagaaa cgggccaggt gataaagcat ccgcgggctg accttgtcct
      121 tactttgaag cttcaagccg gatctgcgcc ataacaaata attgatcact gctgccaaac
      181 caaagccggt acaggcgcct tctgattctt gatcgagaac gagaccggcg gcgagatagc
      241 gcggtagcag cttcgaaata tccttttcgc ccgggtaaat cggcgccaag cagacgaccg
      301 atggacgata gggtaggtcg cgcaggtcaa gacgatccgg ccgcgcatcc agcgtaattg
      361 gccggcccgc aatctcgatg gttctcgtcg gcatctccac tccttccgtc gaccaacatt
      421 gactaacggt ttattagact ctgattaggg actgctcaag atctcgaagg cgacgccgaa
      481 ccgcgtgctc atgtccgtga gaacggatcg aaatttccga acgaacacgc acacgaaaac
      541 acaaaaagta aatatgtcga tacgatgagc tcaccgctcg acgatctcag gacaatcggc
      601 atccggatgt gtcagcagct ctacgccaat gccttccatg ccttcatgcg cgaagtgcca
      661 taaagattgg gagactgggg atattgctgt gaaggaagcg ctttgcgaat ggtttgatac
      721 cattgcttgt aggtcgcctc gggcgacaga ccggaaagcg cgcggatcgc gacaaatgta
      781 aaggcgccgt tggggcggcc ctcgaaggac gtgtcgtagc tatattcggt gtcctggcag
      841 ccggccatca gcagcgctgc gtaacgtccc ggaggattgg aacgccgcac gccgcggccg
      901 atgccgagtt tgccgatatc gcgcttgggt aaaaaagttg ccggtggcaa gaagcgtact
      961 tttcggatcg gcgcgccctt gcctttaata gtcggcggag tggtgatggg cgcgaacttc
     1021 gcgacggttc ccgaatggca ggaatctgaa atgaccaaga gcttaacgcc agccttctta
     1081 ctgctataga gttcgaagag ctcgtcatcg gtaatcggac cttttgattt gatgtcatag
     1141 gggcagatac attcgtcggt gccatccggc tcgtcgccgt ttttatccgg cacatacgac
     1201 ccatgtccgg agtattggat gatgacggta tcgccgcttt ttgctccgcc gatggtcgat
     1261 tcgatggcgc ttcgaatatt tttgccggtt gccttcctat caacgatctg caaaacggca
     1321 aatcccttct tctgaagcag cgcgctccag tctcgaacat cgttgacaca gccggccaga
     1381 tcgcttccgg ttcctggata atcgttaatg ccaatgcaca ctgcgtattt cgccatgaac
     1441 tcctccgttg aaaatgtagg tgtatgttaa tcttcgaagc aaatttcgtc tataaaaaag
     1501 tctcacaggc ttccttgcgc caagttgcct tgctcagatc gtgttcgtgc tacgtgttgg
     1561 tgggggaaat tctcgaccac cttttcatgg cacgatcacg aggcttccga tcacacggta
     1621 tgtgtatcct tgacagcttt gtcgtccatg ttaacttaca ccgtcatcaa tttagttccg
     1681 cttcaggagg atctgcgatg aatggactga ggagttctgt tttaacttcc ttggccggcg
     1741 tcttgctcgc cggtttgtta tcggctgctc cagcgttggc gcagagcgat ttctataaag
     1801 gcaagacgat cactgtgtac atcggcacca ccgcgggagc gctctacgat cagtgggggc
     1861 gcattctcgc tacgcatatg gttaagcaca ttccgggtaa cccgaacgtg atcgtgcaaa
     1921 atatgccggg tgccggccat atgatcgcgg cgaactatgt ctacaaaaca accaagccgg
     1981 acggcttgag cttgatcggt tcgatcgtgc cgacgttgta cttcgaccaa ctggtcgggc
     2041 gcaaggaagc gcaatacgat tgggccaaat tcgtctggat cggttccccg gtgaagggag
     2101 agttgcagat gtatatgcgc gccgacactc cctacaaaac aatggatgac gtgcgcaacg
     2161 ccaaggaacc gccacgctgt ggcgcgcagg ggacatcgga tacggcttac tatgtgccga
     2221 agctgtttga ggaagtaatc ggcacgaagt ttaacctggt gcagggttat cccggcgggc
     2281 cggagattga cttggcggtg gagagaggcg agatccactg ccgcgctttt accattgagg
     2341 catttttctc gcgtgagcca tatcatacct ggcgcaagaa gggctttgtt cgaaacatca
     2401 tccaaaccgg caagaagcgc gacagcaagc tgcccgatac cgaaaccatc tgggagctga
     2461 tggacaagta caaaacgccg gaagcggacc ggcgtctcgc caccgtgatg ttggcatcgg
     2521 gcgcgctcgg ccggccgatg ctggctaccc cgggcgttcc ggcagaccgc gtgaaaattt
     2581 tgcgcgaggc tttcaacaag accatggagg ataaagaatt tttagccgag ctggaaaaac
     2641 gaaagttcga gctcgatccc acgccgggcg aagagctgga aaagttggtg aaagacgtga
     2701 tgagccagcc gcccgaaatc gtcgcccgaa tcaaaaagtt gctgggccaa tagacaaacg
     2761 acaggcgcga aaaagccgcc gtgcgtgaga acgccggcgg cttttttgtg gccgcgcgaa
     2821 gctcagcggt acagcccgtc gatgtatccg gatttatcca gctcttcgag aaagctcatg
     2881 tcgacgaact ccttcgcatt tgccgttcgg gcggccggca gccgttcgct gacatctttg
     2941 aagatggtct tgaaggattc caggttgggg taaggtttgg gtttaacgat ttccagcagc
     3001 gtctgatagg catcttccag ctgcttttcg tctttggtct tgcggtattt gacgaaagcc
     3061 cgcttggcca gctccgggtt agtcctcacg atctgaatcg cgtccacata acttttcacc
     3121 atccgcctgg tcacatcgcg attcttggcg atgtaacgct gtgatgccac caacccggtg
     3181 ccttgataat aaatgttcag ctcggccata tttagcagga ctcgcattcc cgcgtgcact
     3241 gcggcgagat gatcgggagg cgagaagctc gacgcatccg ctgaatttcc cagcaaggcg
     3301 ataatccgcg ccggcgtatc accgatctgt aaaaaggtaa tatctttatc ggtcaagccg
     3361 aagcgcggca acatgttgag cacgcgcata tgtgaagcgg ccccgaaacg ggaaaccgcg
     3421 atgcgtttac ctttgagttc ttccggcgtt ttgatgttgg gccgaaccac caagcgatca
     3481 tcgagcaatt cttgaatgct gaccagcatc acgacatcgc ttccttgcag ccgcgccggt
     3541 gacatgaggg cgccggtcat ctgcccgaaa ttgagctcgc cggatatcaa tgatcccatc
     3601 gagatgtttc ccggcatata gatgatctcg aggtccagat tgtttctctt aaacactccg
     3661 gcgacatcgc cgacccagag aacaccatgc tggggaccgg gcgaagtgta ggcgatgcgt
     3721 atcttgtctg cgccaaagat tgattgggcg gggaaatata gacagagaaa cgccgccgca
     3781 aggaaaagtt tattcgcttt gccggttcgg gagatcatag tggcctccgc ttgtcgctgc
     3841 gatacggtga agaactatcg tgaagagagc agccgcaagg tatcgcgggc ggatgcggcg
     3901 gtcaacaaag atattgtgaa ctggttttat ctgaggcgac ttagccaaca tggttcatag
     3961 agtcataatc gcgctaaacg gtctctggcg gatacaaacc gcgccgcaga tcgcgttcaa
     4021 gcaattcgcg cttgcgttct cggttgaccc gtagccgccg ccgatctccc gtgtttatcg
     4081 acgtatagag catatcgccc tagcagggtg ctgagaaaag cagcctgcta ggcgactttg
     4141 cataagtggg gcggcagcaa ggctacaggc ggcacatggt ggccaagtct cagagcagca
     4201 agaagagctt tcagtatgta taggatggta tggttcggcg gcatcacaat taagcgagta
     4261 gcgccggctt ttgtggattc tcgcacggct ttgctcaacc cgcctgcata catcttaact
     4321 tccgggttaa tcccgttccg ccgaagcgct tcgcacatcg acgctaaatt ttgttctgcc
     4381 ttcacttttt gtaggtcgac gttgaagcgc catgcgcttc cccgatattc tcgcgaggtt
     4441 atgctgccgg tttcgatcat gggcagatga gtttccacgt taccccaggt tgatgtcgct
     4501 gtgcgtatca gaaagatgat tgtgtcaccc ggcttagcgg tcattcttag atgggccatg
     4561 aagcgatgga tgcgctgagt ttcgtcaagg gcgactagaa tacggtccgc cattttgccc
     4621 tccctgagtt caggtcgtat cgaggagtgc gtgtcgggtt tacacggtct tgatgaccga
     4681 actctagcgc aagctacgtg ccgttttggc gagcgggtgc gaaccgt
//
LOCUS       DASUTG010000529         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_378051, whole genome shotgun sequence.
ACCESSION   DASUTG010000529 DASUTG010000000
VERSION     DASUTG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378051"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(364..2184)
                     /locus_tag="VF089_12160"
     CDS             complement(364..2184)
                     /locus_tag="VF089_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174761.1"
                     /translation="MSSLLEKWRQLRQSFTHTPQAVRFVWRTNRWATVGLGLLTLGGA
                     LLPASQAWVGKLIVDGVVASIQTGQDAEQVRRVFIYLLLELGLFLLSAAFNHGRRLIQ
                     QLIQLQLANRIRAEIIGKALTLDLAYFEDSDFYDRLQNARREGGYKPVELINDTFLIV
                     QNMITLISFALLMLRFSPWLVVVLVATGIPAFIAETRYSERGFRLLTLRAPETRQVNY
                     LSRLLTEDTAAKEIKLFNLGGTLLNRYTTLFDKFFEEDKSLALRRATAGFSLGLIATL
                     GFYGSYAWIVWYTVQGKISLGDMTLYLAIFRQGQSTFQSILSALGNIYENNLFMANFF
                     EFLCLEPRMQMPGQGRKLSLPLRDGIEFREVGFRYPDNEEWALRGVNLVIRPGEKIAL
                     VGHNGAGKTTLIKLLSRLYDPTEGSVLIDGIDIRDLDPLELQQRIGVIFQDFVRYHLP
                     VRENIGFGQIDALDDIERIATAARKSGAHAVIEELPEGYETMLGRWFHDGHELSLGQW
                     QKIALARAFMRDAEILVLDEPTASVDARTEYEIFQNFKALTEGKMAILISHRFSTVRM
                     ADRIAVIQDGRIAELGTHDELLRREGTYAELFSMQAEGYR"
BASE COUNT          603 a          684 c          701 g          550 t
ORIGIN      
        1 ctcgctcgac caggagtagg tcaagaccgc attctttacc atcgaccagc gcgggacatt
       61 ttctctcttc catctttgat tcctcctcgt gatcaaacgc gattcactat tcgccttgta
      121 aaaaaagttg ttccagcctg ctcaaagaaa tcagaggtaa ggcgcgcgaa aaaccgataa
      181 atacgttgca gtgatccggt tgagcgcaag aaagcatagg ggtctttcag cggcctgcta
      241 gttcaccttt gcccgctcgt gtatggggtg tcaagtagga ggagccatca cgagcgacaa
      301 gcggtgatgg tctaaatcgg agataggttc aaggctcgca atcttgaatt ttgaaaggat
      361 aactcagcga tatccttccg cttgcatgct gaagagctcg gcataggtgc cttcgcggcg
      421 caagagttcg tcgtgtgtgc cgagctcggc gatgcggccg tcttgaatga cggcaatccg
      481 gtcggccatg cgaacggtgg aaaaacggtg tgagataagg attgccattt tcccttcggt
      541 gagcgctttg aagttctgga agatttcgta ttccgtacgc gcgtccactg atgcggtggg
      601 ttcatcgagc accagaatct cggcgtcgcg cataaaggcg cgagccaggg cgatcttttg
      661 ccactggccc agggagagct catggccatc gtgaaaccag cgcccgagca tggtttcata
      721 accctccgga agttcctcga tcactgcgtg cgcgccgctt tttcgagcag ccgtagcgat
      781 acgttcgatg tcgtccagcg catcgatctg gccgaacccg atgttttctc taacgggcag
      841 gtgatagcgg acgaagtctt gaaagattac gccgattctt tgctgcagtt ctaaagggtc
      901 cagatcacgg atgtcgatgc catcgatcag aacactgcct tcggtgggat cgtaaagccg
      961 gctcaagagc tttataagcg tggtcttgcc cgcgccattg tggcccacca gcgcaatctt
     1021 ctcacccggt cgtatgacga gattgacgcc ccgcagagcc cattcctcat tgtccggata
     1081 gcgaaagccg acttcgcgaa actcgatgcc gtcgcgcaat gggagcgaca gcttgcgccc
     1141 ttgtccgggc atttgcatgc gcggctcaag gcaaagaaat tcgaaaaaat tcgccatgaa
     1201 taaattattt tcataaatat tgccgagggc cgagagaatc gattggaacg tcgattgccc
     1261 ctgccggaaa atcgccaggt aaagcgtcat atcgcccagc gagattttac cctgcacggt
     1321 gtaccagacg atccaggcgt aggagccgta aaatcccaag gtggcaatga gtcccaggct
     1381 gaatcccgca gtggccctgc gtagagccag tgatttatct tcctcgaaga acttgtcgaa
     1441 cagtgtcgtg taacggttga gcaaggtacc gccgaggttg aacagcttga tctctttagc
     1501 tgccgtgtct tctgtcagca ggcgcgaaag ataattgacc tggcgcgttt ccggagcgcg
     1561 cagggtgagc aagcgaaagc cgcgctccga gtagcgggtt tcggcgataa atgccgggat
     1621 accggtcgcc acaagaacga ccaccagcca cgggctgaaa cgcagcatca agagagcgaa
     1681 agagatcaag gtgatcatgt tctgtacgat gagaaaagta tcgttgatca attccaccgg
     1741 cttgtacccg ccctcgcgcc gggcattttg cagccgatcg tagaaatcag aatcttcaaa
     1801 gtaagccaga tccagagtta atgctttgcc gataatctcg gcgcgaatgc gattggcaag
     1861 ctggagctgg atcagttgtt gaatcagacg ccggccgtga ttgaaggctg cgctcagcag
     1921 gaaaagaccg agctcgagca gcaaatatat aaaaactctt ctcacctgct cggcgtcctg
     1981 gccggtttgg atggacgcga ccacgccatc gacgatgagc ttccccaccc acgcttggct
     2041 cgccggcaat aaggcgccac ccaatgtcaa caaccccaat cccacagtag cccaacggtt
     2101 ggtgcgccag acgaagcgta cggcctgggg cgtatgggtg aacgactggc gcagctggcg
     2161 ccacttctct aacaaagaac tcatgtcgtt aggtcgtttg agcagagatt cacgttgccg
     2221 gctgaaacta taaacccgga gcgaggaact taaaactcga agcggcgtaa gcgccgttag
     2281 cgatcactgc gcttcctgaa agcgagggct gcccgtgagg cttcagaaaa aatccagtga
     2341 ccctagggct cataagcatt atgtgtctgc tgtgaggaag aacaatcccc catcgctatt
     2401 cagcggtcct tcacttcgcg gtgatttcac ggagcaaact gattcgctcg aagcccgagc
     2461 tgcgacatcc gcttaaaaag cggcctgaca gccgcgtcgc agcaactcca gtgcgttctt
     2521 tgcttccggg ctgatcac
//
LOCUS       DASUTG010000530         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_525856, whole genome shotgun sequence.
ACCESSION   DASUTG010000530 DASUTG010000000
VERSION     DASUTG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525856"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VF089_12165"
     CDS             complement(<1..508)
                     /locus_tag="VF089_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174762.1"
                     /translation="MNDSQAYKVIGRPEPRSDGAEKVTGKAVYTVDVDLPGMAHAKVL
                     RSPYPHARIGRVDSSKADRLPGVYAVITKEDQRELGMFGAAYKDQTIVAVDKVRYVGD
                     PVAAVAAVDEATAEEALGLIDVEYQEVPAVSSLEEALAPGATLVHDSASLGGELMGQR
                     YEKPEEFAG"
     gene            complement(505..1824)
                     /locus_tag="VF089_12170"
     CDS             complement(505..1824)
                     /locus_tag="VF089_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6174763.1"
                     /translation="MISPDHHAVAIVKRLREEGYESYLAGGCVRDFLLNKNPQDYDIA
                     TSARPEDVQRIFPRTIPVGAQFGVVLVILDGQSFEVASFRFDGPYLDGRRPSEVRYGT
                     LEEDILRRDFTINGMVYDPIENRVIDLVEGKRDLARRCIRAIGDPRQRFEEDRLRMVR
                     AIRFAASLNFDIDSLTLEAIQQSAAMITQISSERIGEEITRILTEGGARRGLEILDET
                     GLLQILLPEIERMKGVEQSPEHHPEGDVFKHTLLTLSHLENPTETLAYGCLLHDVGKP
                     VCFRKEADRITFYGHTDCGAEMAEAILKRLKRGRAVWERVAYLVKNHLRHTQAPKMRL
                     STLKRFLREEGIHELLELARIDALSSSGDLQYYQFCQERLSQMQEEEIRPEPLLRGKD
                     LIEMGYIPGPIFAEILRQVEDAQLGGELTSRQAAMDWVKQNYPPRFQ"
     gene            complement(1880..2266)
                     /locus_tag="VF089_12175"
     CDS             complement(1880..2266)
                     /locus_tag="VF089_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174764.1"
                     /translation="MQKVLIIEDHADMRELLSWQIELMGFMPILAKHGKEGIEKAIAE
                     KPELILMDIMMPGMDGWEAARAVRASPEIKDIPILAATAMFRDSDLKTCMEAGCNSYI
                     VKPFTFLELQSKIRELLPQTKDAVPS"
     gene            complement(2392..3714)
                     /locus_tag="VF089_12180"
     CDS             complement(2392..3714)
                     /locus_tag="VF089_12180"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6174765.1"
                     /translation="MNKILVVVLLVGSLLVSTGFGQAAMTPHRSVLNNGMVLLTSEQR
                     TLPMVSIEMLIHAGARYDPPKQDGLANLTAELITEGTKRRTALQISESLDFLGASLAA
                     RSGEDLASVSLTVLKKDLPAGLELLAEVLTSAVFPQEEIERQKQAVIATIRAKQEQPG
                     EIAEMHFAAAVFPQTPYGRPVEGNEASVKAITQASLREFYERYYRPNRSIIAVVGDIS
                     HQEAIEALNQAFSSWKKGDPPPKPLLPSAAPNVGTIRINKDLTQANIVLGQRGVGRRN
                     PDYYALQVMNYILGGGGFSSRAMDSIRNERGLAYSVYSYFSAEKGRGTFQFVMQTKNE
                     TALQAIDIAKEEIRRIRQTPVSEAELNDAKDYLTGSFPLRFDTMGKVANFLAQVEYFE
                     LGLDYTERYPDLIRKITIDDVRRVAQKYLEPERMITVIVGNQTIIAGK"
     gene            complement(3711..5033)
                     /locus_tag="VF089_12185"
     CDS             complement(3711..5033)
                     /locus_tag="VF089_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6174766.1"
                     /translation="MRGYFYGLIFLLFLLAASPQNSFADVQEFLLENGLKILLLEDHK
                     SPAVTFQVWYRVGSRNELDGKSGLAHFLEHMLFKGTPTTGPEQYSQIIAKNGGQANAF
                     TTADVTVYFATMSRDKIHIQLELEADRMVNALLGEEYFEAEKRVIQEERRLRVDNNPA
                     AALGELTGAVTYIVHPYRRPVIGWMHDIVKLTRQDLVDFYKLYYSPNNAFIVVTGDFS
                     TEEILPKIRAAYGKIPRGPEPPKLIVEEPPQHGERRTVLKKEAELPIVTAYYHAPTLQ
                     NPDNFALDLLTVILAGGRSSRLHQELVYQKRLARGVDADYSGASIDPPVLSVTAQLLP
                     GKDPAAVEREIDGLIEKVKTELVSERELQKAKNQVEAAFTFSRDSIFGQAMKIGFYEI
                     AGDWRLMDRYLEGIRKVSREDIRKVAKHYLDRDRRTVGTLIPIKGKTP"
BASE COUNT         1156 a         1472 c         1301 g         1227 t
ORIGIN      
        1 tgccagcaaa ttcttccggc ttctcatagc gctgtcccat caactcaccg cccagcgaag
       61 ccgaatcgtg caccaacgtc gcgccgggcg ccaatgcttc ttctaaggaa gagaccgccg
      121 gcacttcctg gtattcgaca tctattagac ctaacgcttc ttcggccgtg gcttcatcga
      181 ctgccgccac tgcggctacc ggatcgccga catagcgaac cttatcgacc gcgacaatgg
      241 tttgatcctt ataagcggcg ccaaacatgc cgagttcccg ctgatcttcc ttggtgatga
      301 ccgcataaac gccgggaaga cggtccgcct tgctggaatc aaccctcccg atacgcgcgt
      361 gcgggtaggg gctgcgcaat accttagcat gcgccatccc gggcaaatcg acatcgaccg
      421 tgtaaacagc tttaccggta actttctctg cgccgtcaga tctaggctct ggccggccga
      481 taactttgta ggcctgcgaa tcgttcattg aaacctcgga gggtagtttt gtttgaccca
      541 atccatcgct gcctggcggc tggtcaattc accgccaagc tgtgcatctt ctacctgccg
      601 caagatttcg gcgaagattg gtccaggaat gtatcccatt tcgatcagat ccttgccgcg
      661 taatagcggc tcaggacgga tctcttcctc ttgcatctgg gaaagacgct cctggcagaa
      721 ttggtagtat tgcaaatcac cgcttgagga caaggcatcg atgcgcgcca actccaagag
      781 ctcatggatg ccttcctcac gcagaaaacg cttaagtgtg ctcagtcgca tcttgggcgc
      841 ctgcgtatga cgcaggtgat ttttgaccaa gtacgcgacc cgctcccaaa cggctcgtcc
      901 acgtttgagc cgtttcaata tcgcttcggc catttccgcg ccacaatcgg tatggccata
      961 aaacgtgata cggtcggctt ccttgcgaaa gcagaccggc ttcccgacat cgtgcagaag
     1021 gcaaccgtat gccagggttt ccgtcgggtt ttcgagatgg ctcaaggtca agagtgtgtg
     1081 cttgaagaca tcgccctccg gatggtgctc ggggctctgc tcaacgcctt tcatcctttc
     1141 gatctccggc agcaggatct gcaacaaacc ggtttcgtcc aaaatctcca atccccttcg
     1201 cgcgcctcct tcagtgagaa tccgcgtgat ctcctcgccg attcgttcgg acgagatctg
     1261 cgtgatcatt gccgccgact gctggatggc ctccaaggtt aagctgtcga tatcgaagtt
     1321 caagctggcg gcaaaacgga tcgcccgcac cattctcaat cggtcttcct caaaacgttg
     1381 ccgtggatcg ccgatggcgc ggatgcagcg cctcgcaaga tctctcttgc cttcgaccag
     1441 atcgataact cgattctcga tcgggtcgta taccattccg ttaatggtga aatcacgccg
     1501 taagatatct tcctctagag ttccgtagcg cacctcgctc ggtctgcgac cgtccaggta
     1561 gggaccgtcg aatcggaaag acgcgacttc aaaagactgc ccatccaaga tcactaaaac
     1621 gaccccaaat tgcgcgccca ccggaatagt tcgagggaaa atccgctgaa cgtcctctgg
     1681 ccgggcactg gttgcgatat cgtaatcttg cgggttcttg ttgagcaaaa agtcgcggac
     1741 gcatccgccg gctagataac tctcgtaacc ttcctcacgg agccgcttaa caatagcaac
     1801 agcatggtga tccggtgaaa tcatccggcc tctccagttc caatgactca atgaaaccag
     1861 aagtggagcc aaaaaacgac taagagggaa cggcgtcctt ggtctggggg agcaactcgc
     1921 gaatcttgct ctgaagctcg agaaaggtga agggcttgac aatgtagctg ttgcagcctg
     1981 cttccatgca ggtcttgaga tcggagtctc gaaacatcgc ggtcgcagcc agaattggaa
     2041 tgtccttgat ttcgggggaa gctcgcaccg ctcgtgccgc ttcccacccg tccattccag
     2101 gcatcataat atccatcaag atcaactcgg gcttttccgc gatagccttt tctataccct
     2161 ctttgccgtg cttcgccaag atcggcataa atcccatcaa ttcgatttgc cagctgagca
     2221 gttcacgcat atccgcgtga tcttcaataa tcaaaacttt ctgcatgaac aaaccacttt
     2281 ctagactggg gcagtgctga tcgcgttcag tttagccagg tagagacacc gcacaaatat
     2341 gccgtgccag ctaaccgccg tcaagcattg tgattgggat tcacaaggaa ttcatttacc
     2401 agcgatgatc gtttgatttc cgacgatgac ggtgatcatt ctttccggct ccaggtattt
     2461 ctgtgcgacc cgtctgacat cgtcaatggt aattttgcgg atcagatccg gataacgctc
     2521 ggtataatcc aggccaagtt cgaagtattc cacctgggcc aggaaattgg cgaccttccc
     2581 catggtgtcg aagcgcagcg ggaagctgcc agtcaaataa tccttggcat cgttaagctc
     2641 ggcctcgcta accggcgtct gccggatgcg acggatttcc tcttttgcaa tatcgattgc
     2701 ctgcaaagcc gtctcatttt tcgtctgcat gacgaattgg aacgtaccgc ggcccttctc
     2761 ggcgctgaaa tagctataaa cggaatatgc caggccgcgc tcgttacgga tggagtccat
     2821 tgcgcgggaa gaaaatccgc cgccgccgag gatatagttc atcacttgaa gcgcatagta
     2881 gtcggggttt ctccgaccga caccgcgctg gccgagaact atattggctt gagtgagatc
     2941 cttgttgatc cgaatggttc ccacattcgg cgccgccgaa ggtagcaacg gcttgggcgg
     3001 cggatcgccc ttcttccagg agctaaaggc ctgattgagc gcctctatcg cttcttgatg
     3061 ggagatatcg ccgacaactg ctataattga gcgattcgga cgatagtacc gctcatagaa
     3121 ctctcgcaag ctcgcttgcg tgatcgcctt gaccgaggcc tcgttgccct ccacgggacg
     3181 accatagggg gtctgcggaa ataccgccgc cgcaaaatgc atctcggcaa tttcaccagg
     3241 ctgctcttgc ttcgctcgta tcgttgctat gaccgcttgt ttttgccgct caatctcctc
     3301 ttgcgggaac accgcactag tcaacacttc cgccagcagt tccaaacccg caggaagatc
     3361 ttttttgagc acagtcaagc ttacactcgc gagatcttcg ccgctgcgcg ctgctagact
     3421 ggcgccaaga aaatccaagc tttcgctgat ttgcagagca gtgcgtcgct tggtgccttc
     3481 ggtgatcaac tccgcggtta aattggcaag gccatcctgt ttcggtgggt cataacgcgc
     3541 gccggcatgg attaacatct ctatcgagac catcggcagc gttctctgct ccgacgtcag
     3601 cagaaccatg ccattgttca acaccgatcg gtgcggcgtc atcgccgctt gtccgaagcc
     3661 agttgaaaca agcagtgacc ctacaagcag cacgacaact aaaatcttgt tcatggcgtc
     3721 ttgcctttga tcggaatcaa cgtaccgaca gtgcggcgat cacgatcgag atagtgtttg
     3781 gcgactttac gaatgtcctc ccggctcact tttctaatac cttcgaggta gcggtccatc
     3841 aaccgccaat cgcccgcgat ctcgtagaat ccgattttca tcgcttggcc gaagattgag
     3901 tcgcgactga aagtgaacgc ggcttcaacc tgattcttgg ccttctgcag ctctcgctcg
     3961 ctaaccagct ccgtcttgac cttctcgatt aagccatcaa tctcccgctc gaccgctgcc
     4021 ggatctttac ccggcaacag ttgcgctgtc acggaaagga ccggtggatc aatggaagcg
     4081 ccgctgtagt cggcatcgac gccacgcgcc aaccgcttct ggtaaaccag ctcctggtgc
     4141 agccgggagc ttcggccacc agcgaggatg accgtcaaaa gatccaatgc gaaattgtcc
     4201 ggattttgca aggtcggcgc gtgataatac gccgtcacaa tcggcagttc cgcttccttc
     4261 tttaataccg ttctgcgctc tccgtgctgc ggcggttctt cgacgatcag tttaggaggt
     4321 tccggaccac gcggtatctt gccgtaagct gcccttatct tcggcaagat ttcttcagtg
     4381 gaaaaatctc cggtaacgac gatgaaggcg ttgttaggcg agtagtaaag cttgtagaaa
     4441 tcgaccaggt cctgccgagt gagttttaca atatcgtgca tccaaccgat caccggacga
     4501 cggtaaggat gaacgatgta tgtaacagcg ccggtcagct cgcctagcgc ggcagctgga
     4561 ttattgtcga ctcgcagtcg tcgttcttct tggataactc tcttttcagc ttcgaaatac
     4621 tcctccccca aaagcgcatt caccatccga tcggcttcaa gctcgagctg aatatggatt
     4681 ttgtctcggc tcatggtggc gaagtaaact gtcacgtcgg cagttgtaaa tgcatttgct
     4741 tggccaccgt ttttggcgat gatttgggaa tactgttccg gccccgtcgt tggggtgccc
     4801 ttgaacaaca tgtgctcgag aaaatgcgct aaaccgctct tgccatctag ttcatttcgc
     4861 gaaccgaccc ggtaccacac ctgaaaggtt accgccggac tcttgtgatc ctccagaaga
     4921 agaatcttga gaccattttc gagcaaaaac tcttgaacat cggcaaaaga gttttgtggg
     4981 gacgcggcga gcaggaacag aagaaaaatc aagccataga agtaaccccg catgctgtta
     5041 ccgtaaagga agtcagctac aacttcaaga aatcctggag acaattagag tcgaaggcgc
     5101 aaagatgatt ctgatccagc gaagtgaccg ttggacgcac tctcgaatcc tgggtt
//
LOCUS       DASUTG010000531         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_972441, whole genome shotgun sequence.
ACCESSION   DASUTG010000531 DASUTG010000000
VERSION     DASUTG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972441"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF089_12190"
     CDS             complement(<1..717)
                     /locus_tag="VF089_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174767.1"
                     /translation="MYEQHFGFQESPFSITPDPRFFYANSVYLEAYATLSYGIEAKKG
                     FIAITGEVGTGKTTLLRKLMLSLDKTIQTVLVFNTDVTFNELLRVILRDLGLPTAGRD
                     RLGLVEELNDYLIEQLEHGRTVCLLIDEVQHLSDESLEGLRLLSNLETDRQKLLQIVL
                     MGQPELQAKLDQPQLRQLKQRIALRAELAPLRDEEVGSYINFRLRVAGCDNPDLFDSG
                     AIEKIAFYSKGIPRLVNVICD"
     gene            complement(774..1049)
                     /locus_tag="VF089_12195"
     CDS             complement(774..1049)
                     /locus_tag="VF089_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174768.1"
                     /translation="MKPSPHGEQARLPLAAGRIAAAAARNSDDKAGTKVAPPGLFSVF
                     DRDKDSSQVNISGVLRWSALADWLALYSGLAAVLGAIRAKSEVSSFS"
     gene            1267..1530
                     /locus_tag="VF089_12200"
     CDS             1267..1530
                     /locus_tag="VF089_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174769.1"
                     /translation="MAARYRNHLILVKADFDAESRFWNVRAHIQFNQHITFRDVLITG
                     PKDTFKTQKRAETHIIQEAKKWVDNRLREMANKTKIADTSVGT"
BASE COUNT          602 a          587 c          585 g          599 t
ORIGIN      
        1 atcacagatt acattgacta gccgcgggat gccttttgag taaaatgcaa ttttttcaat
       61 cgcgccacta tcgaataggt caggattgtc acagccggcc actcggagcc gaaaattgat
      121 gtaagagcct acctcttcat ctctcaacgg ggcgagttca gcacgaaggg cgattcgctg
      181 tttgagctgt cttaattgtg gctgatcgag tttcgcctgg agttcaggtt gacccattag
      241 cacgatctga agtagctttt ggcgatctgt ttcgagattt gacagaagcc gcaatccctc
      301 caacgattcg tcgctcaaat gctgaacttc atctatgagc aagcaaacgg ttcggccatg
      361 ctcaagttgt tcgatcaaat agtcgttgag ttcctctacc aagccaagcc ggtctcttcc
      421 tgctgttggt aagccgaggt cccgcaaaat aacccgcagc agctcattga aggtaacatc
      481 agtattgaag acgagcaccg tctggatagt cttgtctaag ctgagcatca gctttcgcaa
      541 gagagtcgtt tttccggttc ctacctcgcc ggtaatcgcg atgaagccct tctttgcttc
      601 gataccatag ctaagagttg cgtacgcctc caagtaaacg gaattggcat aaaaaaaacg
      661 tggatcgggc gtgatgctga agggcgattc ttgaaaacca aagtgctgtt cgtacatgct
      721 gttcgctagc tataggtgat tagcggacga cccaataatt tgtttgaaaa cttttaactg
      781 aaagaactca cctcgctctt tgctcggatc gcgccaagca cggccgctaa accagaatac
      841 aaagcaagcc aatcggctag cgctgaccat ctaagaactc ccgaaatatt aacctgactg
      901 gagtctttat cgcggtcgaa gacactgaac aaccctggtg gagcaacctt tgtgcctgct
      961 ttatcgtcac tgtttcgagc agcagccgca gcgattctcc cggcggcaag cggcaatctc
     1021 gcctgctcac cgtggggaga cggcttcatc gggtttatcc gtcggtaggt tcgaacgagg
     1081 aatgaaaacg gcgatagtgg cttccggcgg ccgtcaaagt actaaccgaa tccgccagat
     1141 cgtagtgcaa gtcgaggcgc ttgagttttc ctattattta gtgtttgtac gatctgtttt
     1201 ctatagccgg gaagtaacta atagtgtaaa tagaatctat tctacccagg aagcgaaagg
     1261 ataagcatgg ccgcacgtta ccgcaaccat ctgattcttg tgaaggctga ctttgacgct
     1321 gagtcgcgct tctggaatgt gagagcgcat attcaattta accaacacat caccttccgt
     1381 gacgttctca tcaccggccc aaaggatact ttcaagaccc aaaagcgggc cgaaacccat
     1441 atcatccaag aagccaaaaa gtgggtcgat aatagacttc gtgagatggc taataagact
     1501 aaaattgcgg atacaagcgt tgggacgtaa gtgcttctag ctcgtctaat gcggtgattt
     1561 agattcaatt tagatgagat ctcctcgtac cgaaggcgct acttcaaaga tcaactaagg
     1621 tgcgtttata attaatgaaa tgaacatgtg tagcggaggg cacgaaggtg gttcctctac
     1681 attcatgctt gccctccgat tatggcatat tgcttgcgag cgatttacct gtgaacgacc
     1741 tactctgtga ccactgtgaa gggcctctga ccggcaatgc atatcgtgta ctgagtgaag
     1801 aagatggcgt cagactgctc gatatggtgg tatgccatgc ttgttattta gaagcgcgac
     1861 ggatcggact caacactgcc gaactcggct cgatcttaca gcagctgccc ggacgtagct
     1921 agaacgatcc ccccgtggga ttctccataa gagctctctt gccgagccaa atgtccgatc
     1981 ggttgtcagt ccgacggtgc atctaatcta tgaacacgaa gctcccgcaa atctaacgtt
     2041 gagattagat acggcaaatc tcggtgatgt cgcttgccct tacgtgtcgt gtacgtggat
     2101 caaccactaa gtttcgttta aacagtcgat cgtttgggtc tcgaaccatc gcactgcctc
     2161 ccggatagga cctccaggtc agcaatcgca cgacagccgt caagggggat gtttaagaga
     2221 gcgatttcaa aaaggtgacg acggcttcag cgtcgcctcg ggttaaccga aacttaggca
     2281 taggagccct tgggatcctc ccatctggtg tcactccttc cattaacaat ttgataccct
     2341 cttgtatcgt atagccgggc agtccggcga tgg
//
LOCUS       DASUTG010000532         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1563737, whole genome shotgun sequence.
ACCESSION   DASUTG010000532 DASUTG010000000
VERSION     DASUTG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563737"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1055)
                     /locus_tag="VF089_12205"
     CDS             complement(87..1055)
                     /locus_tag="VF089_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174770.1"
                     /translation="MKLGMCKITGFLIAFVILGFQSIEAKTLYIGVSNPDMSFLSGGV
                     AYFQGYFKDEGLDVEVLQMNANVSVAALAARNIDYNLILQSVVTANLRGLPLKVAGIL
                     IERPNHAVVVHPSIQKFADLKGKKIAISSFGSLVDILARLTAAHFNLDPKSDVQYVAA
                     GSSSARLTQLQTGVVQAAFLTPPGNLRAEAAGFKTLLNVRDLFAFPVNGIGLHEQKLK
                     NDRDEVKKITRALLRANRFILDNPKGAIKILAAWGRSKLEVAEEAYQYNAKNYSRNLL
                     VSRPTLENVIESTRWNVETKRNVAVDDVFDFSVVREILKEMGETPK"
     gene            complement(1229..1735)
                     /locus_tag="VF089_12210"
     CDS             complement(1229..1735)
                     /locus_tag="VF089_12210"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6174771.1"
                     /translation="MNLELARRTVAALKEAGIDFVSYIPETRLSEILPLMKADKAFTL
                     AAVASEAEAVGIAAGAALGGKGAAVYMEGTGLFVSAYNLLTVGERYGVPILLVVAFVG
                     SFDDQRNSFLFSHYGIKTIGLLKTIGIEHVVVDSGEQLEARVKDAVRMMNALKLPVAL
                     LFTGEFTE"
     gene            complement(1742..2341)
                     /locus_tag="VF089_12215"
     CDS             complement(1742..2341)
                     /locus_tag="VF089_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6174772.1"
                     /translation="MIRYECFEWLAPLMTDQLVVTSLSGQRVEWGHLSKREADLNLGS
                     MGNALAVGIGLALALPHRKVFVFESDGSVLLSLFNLPTLANLNPPNLAVFVFDNQAYS
                     GTKISYPSATAGKTDLAGVAKASGIDHAVTLSKLDDFKKEASAALKESGLRFVVCKIE
                     ESVGHRKITRSNVDLLETKYRFVRYLERTEGKPIFHGRG"
     gene            complement(2338..2844)
                     /locus_tag="VF089_12220"
     CDS             complement(2338..2844)
                     /locus_tag="VF089_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6174773.1"
                     /translation="MEETLGQMLVHSLRAAGIDFITYLPETRLSQIIPLLQNDSHFQL
                     APVGSEAEGVSIAAGAALGGKVVASYMEGTGVYVACYNILTVGVRYGVPMLLLVSYVG
                     SLEDHRNSFLYVQRGIKLIPQLEALDVQYQVIGTGEALERKVKNGVRMMNALKQPVAL
                     LFTEDFTL"
     gene            complement(2846..>3285)
                     /locus_tag="VF089_12225"
     CDS             complement(2846..>3285)
                     /locus_tag="VF089_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174774.1"
                     /translation="EREGFNVLADVGALGLPFLHNGIAASRRFVRDNPDTARRYVKAQ
                     VEAVHLMKTDRKTSVAVLGKYLRQTANKEVLERSYELSSTDDKYPRKQYPSLPGIQTV
                     LDAIADENPKAKSARPEQFVDTRFIKELDDSGYIDSLYKGKAK"
BASE COUNT          743 a          927 c          851 g          764 t
ORIGIN      
        1 aatttttcgg ctaaggataa tcagcatgaa agccctcggg agtgtgatgt tgtcgcctcg
       61 atgatcgcgc gcagtgccag ccgcagctac ttaggggtct cacccatttc cttcaagatc
      121 tcgcgcacga cactgaagtc aaatacgtcg tcgaccgcga cgttcctctt ggtttcaacg
      181 ttccaacgcg tgctctcgat cacattctca agcgtgggcc gggacaccag gagattgcgg
      241 ctatagttct tagcgttgta ttgataagcc tcctcggcga cttccaattt ggatctgccc
      301 caagcggcaa gaatcttgat cgcccccttg gggttatcca agataaaccg gttggcccgc
      361 agcagagcgc gggttatttt ttttacttcg tctcgatcgt ttttgagctt ctgctcgtgc
      421 agcccgatgc cgttcaccgg aaaagcaaac agatcgcgca cgttcaacaa cgtcttgaag
      481 ccggcggctt ccgcgcgcag atttcccggc ggagttagaa atgccgcctg aacgacgccg
      541 gtttgcaatt gcgtcaggcg cgccgaactg ctgccggcgg cgacgtattg aacatcgctt
      601 ttcgggtcga gattgaagtg cgctgccgtc agccgggcga ggatatcgac cagggagcca
      661 aacgagctaa tcgcgatctt cttacctttg agatcagcaa acttttgaat gctcgggtga
      721 accaccaccg catggtttgg ccgctcgatc aaaatgccag caacctttag aggaagccca
      781 cgcagattcg cggtgacgac ggattgcaaa attagattgt agtcgatatt gcgggccgcc
      841 agcgctgcaa ccgagacgtt ggcgttcatc tgcagcactt cgacatccag cccttcgtct
      901 ttgaaatagc cttggaagta agccacaccg ccgctcaaaa acgacatgtc cggattggac
      961 acaccgatgt aaagcgtttt cgcttcaatg ctctgaaatc ctaaaataac gaaagcaatc
     1021 aaaaagccgg ttatcttaca cattccaagt ttcatctttc attctcccgt tgcaagttga
     1081 ttgtaataag caacaagttc ctgcaggctg ctcaaaagat ctcataggcg aggcgcgcga
     1141 aaaatcgacg agcgaaggcg tattcttcag tacgtcgatg cgggcgattt gagcccaacg
     1201 aaggatatga ggctttttca tcagcctgct actcggtaaa ttcgccggtg aataaaagag
     1261 ccaccggcaa cttcaaagca ttcatcatgc gcacggcgtc tttgactcgc gcctcgagct
     1321 gttcgccgga gtcgaccacg acgtgttcaa tgccgatggt ctttagcagg ccgatcgtct
     1381 tgatgccata gtgagaaaac aaaaaagagt ttcgctgatc atcgaagctg ccgacgaaag
     1441 ctactaccaa aaggatcggc acaccatagc gctcgccgac ggtaaggagg ttataggcgg
     1501 agacaaacag gcccgtgcct tccatgtaga ctgcggcgcc cttgccgcct aacgccgcgc
     1561 cggcggcaat gccgaccgcc tccgcttcac tcgccaccgc tgccagggta aaagctttgt
     1621 ccgctttcat cagcggcagg atttcactca accgggtctc gggaatgtaa ctgacgaaat
     1681 cgattccggc ttctttcagt gcggcgactg ttctacgggc gagctcgagg ttcatgatcg
     1741 gttaaccgcg accgtgaaaa atcggtttgc cttcggtgcg ttcgagataa cgcacgaaac
     1801 gatacttggt ctccaacagg tcgacgttgc ttctggtaat cttgcggtgt cccacgctct
     1861 cttcgatttt acagaccacg aagcgtaagc cgctctcctt gagagcggcg cttgcttctt
     1921 ttttgaagtc gtccaatttc gatagggtga ccgcatggtc gatgcccgaa gctttcgcta
     1981 ctcccgccag atccgtctta cccgctgtgg cgctggggta gctgatcttc gtgccgctgt
     2041 aagcctgatt gtcgaaaacg aaaaccgcca aattcggcgg atttagattc gccagagtcg
     2101 gcagattgaa cagagaaagc agcacgctgc cgtcggattc gaagacaaaa acttttcggt
     2161 gcggcagcgc cagcgccaga ccgatgccca cggcaagggc attgcccatg gaaccgaggt
     2221 tgagatcggc ctcccgcttc gacagatgtc cccattcgac cctttgaccg ctaagcgacg
     2281 tgactaccag ctggtcggtc atgagcggcg ccagccattc gaagcattcg taccgaatca
     2341 tagggtaaag tcctcggtga acaacaaagc caccggctgt ttgagcgcat tcatcatacg
     2401 cacgccgttt ttcactttgc gctcgagcgc ttcgccggtg ccgatcactt gatactgcac
     2461 atcgagagct tccaattgcg ggatcagctt gatgccgcgc tgaacataaa gaaaactgtt
     2521 tctgtgatct tcgaggctgc cgacatagct aaccagtagc aacatcggca cgccgtagcg
     2581 aactccgact gtcaagatgt tgtaacaagc aacatagacc ccggttccct ccatgtaact
     2641 cgcgacgact ttgcccccga gagcagcgcc cgcggcaatc gacacccctt ccgcttcgct
     2701 gcccaccggc gccaactgaa aatggctatc gttttgcagc aacggtatga tctggctgag
     2761 ccgggtctcg ggcaggtaag tgatgaagtc aatgcccgcc gcgcgcaaag aatgcaccag
     2821 catttgcccc agggtttctt ccatcctatt ttgcctttcc tttataaagg ctatcgatgt
     2881 aaccgctgtc gtcgagttcc ttaataaagc gcgtatcaac gaattgctcg ggccgcgccg
     2941 acttagcctt ggggttttcg tcggcgatgg cgtcaagaac ggtctgaatt cccggcaacg
     3001 acggatattg cttgcgcgga tatttatcgt ctgtggacga gagctcgtaa cttctttcga
     3061 gaacttcttt atttgcggtc tggcgcagat acttgccgag tacggcaacg ctggtttttc
     3121 gatcggtctt catcaggtga accgcttcga cctgcgcttt gacgtagcgc cttgccgtgt
     3181 caggattatc tcgaacaaag cggcgcgatg ccgcgatacc attgtgcaaa aacggcaatc
     3241 ccagagcgcc gacatcggca agcacgttaa atccttctct ctccg
//
LOCUS       DASUTG010000533         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_972728, whole genome shotgun sequence.
ACCESSION   DASUTG010000533 DASUTG010000000
VERSION     DASUTG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972728"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF089_12230"
     CDS             complement(<1..567)
                     /locus_tag="VF089_12230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174775.1"
                     /translation="MKYNIKICALGAALLFAMSSATIAFGAELSKVRFAQSGHTAASW
                     PIYIAQEKKILEKNGIQFENIIIQGATNTTRAVLSETIPLGRINPDYVIGAIEKGAKV
                     RIIGGNMEKIPYDLMARSEIKSGAELKGKTIGVSTLTGCTTNMVEEVLLKGFKLKEGD
                     YRYLVVGTSPERYAALKAGSVQATFMGPP"
     gene            complement(593..1393)
                     /locus_tag="VF089_12235"
     CDS             complement(593..1393)
                     /locus_tag="VF089_12235"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6174776.1"
                     /translation="MSGPALLLRNLNKAITRPDRDPFVIFRDINLSVSDGEFVSIVGP
                     SGCGKTTLLRVINGLMPHSGGEILIDGRSAHELDNELVMGFVFQGASLLPWRTSLKNV
                     VLGLEGRNNNAKESERTAQKFLDLVGLSGFETHYPHELSGGMQQRVNLARALAVNPRI
                     LLMDEPFAALDAQTRNFMQLELLRIWRETKKTVIFVTHMIAEAILLSDRVIVFSHRPG
                     SIRSEVNVPLPRPRTMEMRSDPRFLELEQVLWKQIEEEVNASGGFSQM"
     gene            complement(1396..2178)
                     /locus_tag="VF089_12240"
     CDS             complement(1396..2178)
                     /locus_tag="VF089_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174777.1"
                     /translation="MNQRNRKLTSTFLKAGSVIGGLVLWHVLATVVVKDTTLLVSPVV
                     VAVTAYQMLFVTKDLYPHIFASSWIFLYGFILAILAGVPFGFLMALNTAVRDYVNPWM
                     ATLYTTPRIAFAPVLLLWLGIGAGSKIAIVFLGCFFPILINSYYGMRVVNRDYVELAR
                     SFRLSRSALFFKILLPASVPFILAGIRLSVGRGLTGVAIAEWFGATEGLGYLIFFAGQ
                     TLNIPILFVGVAVFAALGILGFEVIRIIEVYALPWRKQPQGS"
     gene            complement(2200..2982)
                     /locus_tag="VF089_12245"
     CDS             complement(2200..2982)
                     /locus_tag="VF089_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6174778.1"
                     /translation="MSRLLPKKEIVRGSLSIIIGLAIWEISARLLLDNELLIPPPSSV
                     LVTFWNLASSGELNKHLVATLIEFICGFITACLIGIIIGYLMGMYKWFDEIMDPWIAT
                     LYSIPIITVVPLIIIWFGIGIFSKVVVVFKITAVAIILNTAAGIKSLDPVWLELAKSL
                     RLSDWQTTYKIRLPGALPFIITGMRLGVGRALLGVIVAELMAANAGLGFLLRDSSETW
                     DSPKLFVTVILLATMGLLSFNLIKKLEQRMAPWRQSAEWSSE"
     gene            complement(2983..5499)
                     /locus_tag="VF089_12250"
     CDS             complement(2983..5499)
                     /locus_tag="VF089_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174779.1"
                     /translation="MNRSWISALALSLVLSFIDGYLDAAQAQDPQAIIERILNPLPEF
                     DPFEKPPAAAPQFFPDEVDKRARELLIDSLTNRDAAIHDHVTFFHAEDRRLQKQHGSS
                     TGLAEHAQDLLNNTIDERERYLAAQRQALKNTSSPERKKYLQAIIDEDDLTRADHLTR
                     QSSVNELGSAFNRLLSSVDLIGVASGNYIGAAAETAIGQAYSLLNTEMSVEERRALAR
                     HLDHLKRHPNDPRNGKIVKEVEELEKKKTAALIRKQLDHSKQAAQKGDVDKALFYAEI
                     ASFLDGRSKIAQTELQTVSKSHLQQIEQRSLGLSAQAEKAASVEQQEDVEELLQALSL
                     RDQDKIAEQAAHLEKKYAGKPLAESARDASSVALEMQGRHEEAKKIIERLASSAKTPG
                     VQKRAAALLQSPEYNLLASLHEARSERRAESVKYVLLGEDLLKKNLIYAAGAVAAAGP
                     AGAVTLGAANAMLLGNNLINVLSNNPVSAQSVIDAGVAYVRNHPGSVNATEVYKILAD
                     TYEEKGMIEKAISYHELAGTPKEKLSSLKQKAATALLNAATKSKGRGTQEYYLTTLLD
                     QYPESPAAAEATKKLAQLATTDSRGLRMSKQFLKENPELFGPAGLGLKASLFDGDPRN
                     MELADRGVNVTGDNELLVYYQTPSGVRSQSYPLSRQTSERFFVALRQKNQAVALADAG
                     QRAKGSIGGIKNLPMPIIQGARPNTGESEGERDDTTFSFVREASAHAPAFPKVLDHEM
                     LSENERNPGGKFSLPPIQGSVSPNRFSMSGSLPAGLWGSQIGIGGDHKGSFAGVQLPI
                     PLLQGFIPVDFMVQGRPGGVSVYPRIHMRDDNGEDRELYK"
BASE COUNT         1189 a         1531 c         1546 g         1263 t
ORIGIN      
        1 cggcgggccc atgaaggttg cctgcaccga accggccttt aacgcggcat agcgctccgg
       61 tgaagtgccg acgaccagat atcggtagtc cccttctttc agtttgaagc ctttgagcaa
      121 gacctcttcg accatgttgg tggtgcagcc ggtcaaggtg ctgacgccaa tggttttgcc
      181 tttgagctcg gcgccgcttt tgatctcgga tcgggccatc agatcgtagg gaatcttttc
      241 catattgccg ccgatgatcc tgaccttggc tcccttttcg atggctccga tcacgtaatc
      301 cggattgatc cggccgagcg gaatggtttc gctgagtacg gcgcgggtag tattggtggc
      361 gccttgaata atgatgtttt cgaattggat accgtttttc tccagaattt tcttttcctg
      421 ggcgatgtaa attggccaac tggccgcggt gtgcccgctc tgggcaaaac ggaccttgct
      481 caactcggcc ccgaaagcga ttgtcgctga cgacatcgcg aatagcagcg cagcgccgag
      541 cgcgcagatc ttaatattgt atttcatgaa tcctccttga gtttgcgggt gtttacatct
      601 gtgagaaacc gccggaagcg tttacctctt cctcgatctg cttccacagc acctgctcca
      661 gttcgagaaa tcgaggatct gatctcatct ccatggttct tggacgaggc agcggcacat
      721 ttacttcaga acggatgctg cccggacgat ggctaaagac aatcactctg tctgaaagaa
      781 gaattgcttc ggcaatcata tgggtgacaa agatgacggt ttttttagtc tcccgccaga
      841 tgcgcagcag ttcgagctgc ataaagttgc gcgtctgtgc atccaatgca gcgaaaggct
      901 catccatcag caaaattctc gggttgaccg ccagcgctct ggcgagattg acccgttgct
      961 gcatgccccc ggaaagctcg tgcgggtagt gagtctcgaa tcccgaaagg ccgaccagat
     1021 cgagaaattt ttgggccgtc cgttctgatt ccttggcgtt gttattccgc ccttccaaac
     1081 cgagaaccac atttttgaga ctggtgcgcc aaggcaggag agatgcgcct tgaaagacaa
     1141 agcccatcac caattcgttg tcgagttcat gcgcggacct gccgtcgatg agaatttctc
     1201 cacccgaatg cggcatgaga ccgttgatga cgcgcagtag ggtggtttta ccgcagccac
     1261 tggggccgac gatgctgaca aattctccat cgctaaccga cagattgatg tccctaaaga
     1321 tgacaaacgg atcgcgatcc ggtcgggtga tcgccttgtt caagttgcgc aataacagtg
     1381 ctggtccgct catagtcaac taccttgggg ctgctttctc caggggagcg catagacttc
     1441 gataatccgg atcacttcga atcccagtat cccgagtgcc gcaaataccg ccactccgac
     1501 gaataaaatg ggtatgttga gagtttggcc ggcaaaaaag atcagatagc ccaaaccctc
     1561 cgttgcgcca aaccactctg cgatggccac tccagtgagg ccgcggccga ccgacagacg
     1621 tatgccagcc agtatgaaag gaacggacgc tggcaagagg attttgaaaa acaatgccga
     1681 gcggctcagg cgaaacgatc gggccagctc gacatagtca cgattgacta cgcgcatacc
     1741 gtagtaggag ttgatcagaa tgggaaagaa acaaccgaga aagacgatgg cgattttgga
     1801 gccggcgccg atgcctagcc agagcaacag caccggcgcg aacgcaattc gcggggtcgt
     1861 ataaagcgtc gccatccatg ggttgacgta atcccgcacc gcggtattga gcgccatcag
     1921 aaaaccgaag ggcacgccgg cgagaattgc cagtatgaag ccatagagaa agatccatga
     1981 gctggcgaat atatgaggat agagatcttt agtgacgaag agcatctggt aggccgtgac
     2041 ggctacgacc accggggata ccagcagggt tgtgtctttt acgaccacgg tcgccagaac
     2101 atgccaaaga accagtccgc cgatgacgga gccggccttt aggaaagtgg atgtcaattt
     2161 gcggttgcgt tggttcataa gccgtgcgcc gggtaccgat cactcagaag accattcggc
     2221 gctctgacgc caaggcgcca tgcgctgttc gagtttcttg atcaggttaa aacttagaag
     2281 tcccatggtg gccagcaaaa tcacggtgac gaagagcttc gggctgtccc atgtctcaga
     2341 ggaatcgcgg agcagaaatc ccaagccggc gttggccgcc atgagctccg cgacgatgac
     2401 gccgagcagc gctcggccga ccccaagcct catgccggtg attatgaagg gcagtgctcc
     2461 gggtagccga atcttataag tcgtctgcca gtcgctgagc cgcagcgact tggctaactc
     2521 gagccacacc ggatccaggg atttgattcc ggcggcggtg ttcaggataa ttgccaccgc
     2581 ggtgatctta aaaaccacga cgactttgga aaagatgccg atgccgaacc agatgatgat
     2641 cagaggcacg acggtaataa tcgggatcga gtagagcgtg gcaatccacg ggtccatgat
     2701 ctcatcgaac catttataca tccccatcaa gtagccgatg ataattccga tcagacatgc
     2761 cgtgataaag ccgcagataa attcgatgag agttgcgacg agatgcttgt tgagctcgcc
     2821 gctgctggcg agattccaaa acgtgacgag cacactgctc ggcggcggga tgagcagctc
     2881 attgtcgagc agcaggcgag ccgagatctc ccagatcgcc aggccgatga tgatggacaa
     2941 agatccgcgg acgatttctt tcttgggcaa gagccggctc atctatttat aaagctcccg
     3001 atcttcgccg ttgtcgtcgc gcatgtggat tcgaggataa accgacacgc cgcccggcct
     3061 gccttgcacc atgaaatcca ccggtatgaa tccctgcaac aacggtatcg gtaattgcac
     3121 gccggcaaac gaacccttgt gatcgccgcc gatgccgatt tgacttcccc agagcccggc
     3181 aggcaaagag ccgctcatgc tgaaccggtt gggcgaaaca cttccctgaa tcggcggcaa
     3241 ggaaaacttg ccgccggggt tgcgctcatt ttcgctgagc atttcatgat ccagaacttt
     3301 ggggaatgcg ggtgcatggg cgcttgcctc gcgcacgaaa gaaaaggtcg tgtcgtcccg
     3361 ttcgccttcg gactcgcccg tgttcgggcg cgcgccttgg atgatcggca tcggcagatt
     3421 cttgatgccg ccgatgctgc ctttggcccg ctggccggca tcggccaaag cgaccgcttg
     3481 gtttttttgc cgcagcgcaa caaaaaagcg ctccgaggtc tgacgtgaca acgggtagct
     3541 ctgactgcga acaccggaag gcgtttgata atagaccaga agctcattat cgccggtgac
     3601 attaacgccg cgatcagcca gttccatgtt gcgcggatca ccgtcaaaca acgatgcctt
     3661 aagcccgagc ccggcaggcc caaagagctc cgggttttct tttaaaaatt gtttgctcat
     3721 acgcaagccg cggctgtcgg tcgtggccag ctgggctagc ttcttggtgg cctctgccgc
     3781 cgccgggctt tcaggatact ggtcaagcag ggtggtcaga taatattcct gcgtccctct
     3841 gcctttgctc ttggtggccg cgttcagcag cgctgttgcc gccttctgct ttaaggatga
     3901 aagcttttcc ttgggcgttc cggcaagctc atggtagctg atcgcctttt cgatcatgcc
     3961 tttttcctcg taagtgtcgg caagaatttt ataaacctcg gtggcgttga ccgaacccgg
     4021 gtgattgcgc acgtaggcga caccggcatc gatcaccgat tgcgccgaga ccggattatt
     4081 gctcaacacg ttgatcagat tgttgccgag caacatggca ttggccgcgc cgagggtgac
     4141 cgccccggcg ggaccggcgg ccgcaacggc gcccgcggca taaatcaggt tcttcttcag
     4201 cagatcttcg cctaaaagga catacttcac cgactcggcg cggcgttcgc tgcgcgcctc
     4261 atgcaagcta gccagaagat tgtactccgg actttgtaaa agcgccgcgg cgcgtttttg
     4321 cacgcccgga gtctttgccg aagaagcgag acgctcgatg attttctttg cctcttcatg
     4381 ccggccttgc atctcgagag ctaccgatga cgcatcgcgc gcggactcgg ccagcggctt
     4441 gccggcatat tttttttcca gatgagcggc ttgctctgcg atcttgtctt gatctctcaa
     4501 gctgagcgcc tgcagcagct cttcgacatc ctcctgctgc tcaaccgaag cggctttctc
     4561 ggcttgtgcc gagagtccga gactccgttg ctcgatctgt tgcaggtggg acttggacac
     4621 cgtttgcagc tcggtttgcg ctatctttga tcgaccatcc aaaaaggatg cgatctcggc
     4681 ataaaacagc gccttgtcga catctccctt ttgcgcagct tgctttgaat ggtccagttg
     4741 tttgcggatc agggcggcgg tctttttctt ttcgagctct tcgacctcct tgacgatttt
     4801 gccgttgcgc ggatcgttgg gatggcgctt gagatgatcc aggtggcggg ccaacgcgcg
     4861 ccgctcttcc accgacattt cggtattgag aagcgagtaa gcctgtccaa tggccgtctc
     4921 tgccgccgcg ccaatgtaat ttcccgaagc cacgccgatc aggtccaccg agctcaacaa
     4981 ccggttgaat gcgctgccca actcattgac cgagctttgc cgggtcaaat gatccgctcg
     5041 tgtcagatcg tcctcatcga taatcgcctg cagatacttt tttcgttccg gcgacgaggt
     5101 attcttcaaa gcttgccgct gcgccgcgag ataacgttcg cgttcatcga tcgtattgtt
     5161 caggagatcc tgggcgtgct cggccaatcc agtgcttgat ccatgttgtt tctggagccg
     5221 gcggtcttcg gcatgaaaga atgtcacatg atcgtggatc gctgcatcgc ggttggtcag
     5281 tgagtcgatc agcagttcac gcgcccgctt gtccacttcg tcggggaaaa actgcggcgc
     5341 cgccgccggc ggcttttcga aaggatcgaa ttcaggcaac ggattcaaaa tgcgttcgat
     5401 gatcgcttgc gggtcttgcg cctgcgccgc atcgaggtaa ccatcgatga agctgagaac
     5461 cagcgacagc gctagagcgc tgatccatga tctattcatc ggcttagtct agtgactttc
     5521 tcaattagg
//
LOCUS       DASUTG010000534         6910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2009423, whole genome shotgun sequence.
ACCESSION   DASUTG010000534 DASUTG010000000
VERSION     DASUTG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009423"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VF089_12255"
     CDS             <1..285
                     /locus_tag="VF089_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174780.1"
                     /translation="IADMRNDYVDWLTRRGDELPVRLRSASGASASFEDICARHAVVA
                     DSDSALQTLKTLTAGTGATHLLAWFNIGNVPHALVQESMQQFAAEVMPHL"
     gene            308..661
                     /locus_tag="VF089_12260"
     CDS             308..661
                     /locus_tag="VF089_12260"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6174781.1"
                     /translation="MEDLTEQKCEACRVGAPPVTADEIRELHPRVPGWQIITEDSISK
                     LDRQFKFKNFKDALAFADAVGAAAEEEGHHPRITTEWGKVGVTWWTHKIRNLHKNDFI
                     MAAKTDAIYKNYQQS"
     gene            complement(1016..2629)
                     /locus_tag="VF089_12265"
     CDS             complement(1016..2629)
                     /locus_tag="VF089_12265"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEX6174782.1"
                     /translation="MAFRIILILLLGLLSGGVQAAPAPDIEEQTRAISAELRCVVCQN
                     LSVADSPSEMAQQMRGSVREQLQAGKSPQEIKDFFVSKYGEWVLLAPTNTGFNRLLWW
                     LPLTVLIIGVIAALLLARRWAARKAHRVAPEIDPSIADRLRKEANAANVQAPDVDDST
                     ATAQLVRESLRLADELKELEFDFQSGKLSQADYSALRSEVEGKQALVLQQLNSLPPEP
                     GVKAPAKVASRRRQERPGRTRFRRWQLATGGAFLLLFGLALGVLLTQSLRPRTSEQDS
                     MTGDFLTGTTPGNAATRSALEEGKRAFAKQDFPKAIEAFKKVLETDPNQPEAHSYMGM
                     ILIQAGHADGAMMAFDRALAQAPNYPMALWGKGMILYREKKDYDGARVVLQQLLQLIP
                     PGEDKTEIEKIVASFPPPGSDAGQVSGAPSPDPRQTVSGKITLGPKVKGMNGKATLFI
                     IARAAGTGGPPLAVKKIDQPVFPLSYVIGAENVMMQGTPFTGKINLSARLDTDGNPTT
                     RTPGDLMGNHHNPVEVGARNIDIVIDQAL"
     gene            complement(2634..3200)
                     /locus_tag="VF089_12270"
     CDS             complement(2634..3200)
                     /locus_tag="VF089_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6174783.1"
                     /translation="MPWRRVAFTLLIIAPILALLAFGFSRDARYITSPLLAKPATPFS
                     IELFNGSKLRLEDLRGKAIFLNFWASWCPPCRAEARDLEAAWQKLKDKNVIFIGVALQ
                     DTDQASLEFLKEFGVTYPNGKDESGRIAIDYGVWGIPESFFIDPQGRITYKHVGGIRA
                     AVVTEKLVEAEQGLVSAKEGRGEFQSVR"
     gene            complement(3407..5368)
                     /locus_tag="VF089_12275"
     CDS             complement(3407..5368)
                     /locus_tag="VF089_12275"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX6174784.1"
                     /translation="MTATIGYISILIALLAAGAGIVAPVLSVRRGHARYSSVIGRAIA
                     GQFIFVTLAASALIFALVSADFSVRYVAFNTTLATPIYYRVTGLWGALEGSLLLWEWI
                     LIIFSGLIAWIYRDRHKETLPWVLMVFSIVSFFFLAVIAFVSNPFETLSPVPADGRGL
                     NPLLEDANMMTHPPLLYTGFVGLTVPFAFAIAALIVGKLDEAWITTTRRWTIIAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSIQVQERRRMLKVWNL
                     SLIIIAFSLTIFGTFLTRSGILSSIHAFSSGPVGIFFLGFLAFVLLASFGLLAYRSDM
                     LKEQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLLSEAVAGVQVSVGAPYFNS
                     VTVPLFLFMVFLMGVGPLIAWRRASWDNLRRNFLWPAIGSLIFGLFLFVWKVKDFLPL
                     LGFTLLGFVVMTIVYDTALALRARRRLANEGIISGLITLTRRNQRRYGGLVVHLGVVL
                     IIMGIAGSMTYSTEREATLGLNESLSIGNYQIRFEGLHGSQQPTHYRVEGAFRVLKQG
                     SDIGLLSPALKFFPTQQSPIGRAVHRSSPSEDIYLILSGFSDLDKNQATLKVLVRPLV
                     VWMWIGGFVIAFGTLVTICPMCKPSASET"
     gene            complement(5365..5808)
                     /gene="ccmE"
                     /locus_tag="VF089_12280"
     CDS             complement(5365..5808)
                     /gene="ccmE"
                     /locus_tag="VF089_12280"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX6174785.1"
                     /translation="MARNRRFLIGGIIILGALSYVIYGGMQQALVYFVTPSELKAKET
                     SSMDKFLRMGGMVVKGSLQKDVKNLTYEFELTDGSSTFPVFFRGVPPDLFREGTGAVV
                     EGRIGKDGVFQASTIMAKHAEEYSPHEDSQATSNSFVPAKEYTSK"
     gene            complement(5951..6655)
                     /gene="ccsA"
                     /locus_tag="VF089_12285"
     CDS             complement(5951..6655)
                     /gene="ccsA"
                     /locus_tag="VF089_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX6174786.1"
                     /translation="MKEQPKKRSSGVLGSLALIIIALGLYWALVQAPRDALQGDVQRI
                     MYLHLPSILTAYLSFFLVFVGSCLYLWKREKRDDNLAHSAAELGVLFTALTIIEGSIW
                     GKPTWGVWWTWDARLTLTAILLLIYSGYLMLRSLIDDEDRAALSAAILGIIGFLDIPL
                     IHMSVYWWRTLHQPPSILRPDKLPWENVHPAMLTALAINFVGFLLLYFYLLSLRLRLG
                     EVRSEIKARRLNQGAF"
     gene            complement(6655..>6910)
                     /locus_tag="VF089_12290"
     CDS             complement(6655..>6910)
                     /locus_tag="VF089_12290"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX6174787.1"
                     /translation="TVGFCVLGTVMSALTLRARARELLLPLVLFPLMIPVILATIQCL
                     GTVLRDGEIGDALPWLRLLIGFDVIFLTLGVLVFDWVIEI"
BASE COUNT         1728 a         1899 c         1892 g         1391 t
ORIGIN      
        1 atcgccgata tgcgcaatga ttatgtcgat tggctgactc ggcgcggtga tgaattgccc
       61 gtccggctga gaagcgctag cggcgcctcg gcgagtttcg aggatatctg cgcgcgccat
      121 gcggtagtcg cggattccga ctcggccctg cagacactta aaacattaac cgccggtacc
      181 ggtgccaccc atcttctcgc ctggttcaac atcggcaacg tgccgcacgc gctggttcaa
      241 gaatcgatgc agcagttcgc ggctgaagtc atgccgcatc tgtaatattt ttcaccggag
      301 gatcgatatg gaagatctaa ccgagcagaa atgcgaggca tgccgggtgg gcgcaccgcc
      361 cgtcacagcc gatgagatac gagagctcca ccccagagtg cctggctggc aaatcattac
      421 tgaagacagc atttccaagt tggaccggca gttcaaattc aagaacttta aagatgcgtt
      481 ggccttcgcc gatgcggtag gagcagcagc ggaagaagag ggccaccatc cgcgtatcac
      541 cacggaatgg ggcaaagttg gggtgacctg gtggacgcac aagattcgca atctacacaa
      601 aaacgacttc atcatggcgg caaagaccga cgcgatctac aagaattatc agcagagtta
      661 gacaatcaag caggggaagc tgaaattctc tccactcccc gcagatgttg tacggcctgc
      721 cagcctcagt aattcactca gcgattaaag agttggagat ccaatgtttc gctaattctt
      781 gcctggtgtt aaatgaagcg atccttcagg gcgtgtataa atcgagccac cattcatcgc
      841 gcgcatgaag caggtgccgg tgccataact cgaacccttc cagctgtttc agtcgttcat
      901 tagttccact ggtttttgaa cttacaagta attaaccgga ccttggtgat gtcaatgatc
      961 tcgccgagta gtgcgtgtga agaagctgga acggacagag gattggaacc ctgatctaaa
     1021 gggcctgatc gataacgatg tcgatgttcc tagcgccgac ctcgaccggg ttgtggtgat
     1081 tgcccatcag atctcccggc gtgcgcgttg tcggattgcc atctgtgtcc aaccgcgcgc
     1141 ttaagttgat cttgccggta aacggggttc cctgcatcat gacattctct gcgccgatga
     1201 cataggataa cggaaaaacc ggctgatcga tttttttgac cgcgagcgga ggtcctcccg
     1261 taccggcagc cctggcgatg atgaataggg tcgccttgcc gttcatgcct ttgactttgg
     1321 ggccgagagt aattttgccg ctgacggttt gtctcggatc cggcgaaggc gcgccagaga
     1381 cttgcccggc atcgctcccc ggcgggggaa acgatgcgac gattttctct atctccgttt
     1441 tgtcttcgcc gggtggaatg agctgcaata actgctgtaa caccacgcgc gccccgtcat
     1501 aatctttctt ttcgcgatac agaatcattc ctttacccca gagcgccata ggataattag
     1561 gcgcttgcgc caaagctcga tcgaaagcca tcatcgcgcc atcggcatgc cctgcctgaa
     1621 tcagtatcat gcccatatag ctgtgcgcct cgggctgatt gggatcggtc tccaaaactt
     1681 tcttgaatgc ttcgatagct ttgggaaaat cctgtttcgc aaacgctctc ttgccctctt
     1741 ctaaagcgga acgggtcgcg gcattgccgg gcgtcgtgcc cgtgagaaag tctccggtca
     1801 tcgaatcctg ctccgaggtg cgtggacgca gggactgagt caacaaaacg ccgagcgcca
     1861 gaccgaagag aagaaggaag gctccgcctg ttgccagttg ccagcgccgg aagcgagtgc
     1921 gacctgggcg ctcttgccgg cgacgcgatg caacctttgc cggtgctttg accccgggtt
     1981 ccggcggcag tgagtttagc tgttgtagaa ccagtgcttg cttgccttcc acctcgcttc
     2041 ttaaggcgct gtaatcggct tgtgacagtt tgccggattg aaaatcgaat tccagttcct
     2101 ttaactcatc ggccagccga aggctttcac gtaccagttg cgccgttgcg gtggaatcgt
     2161 cgacatcggg cgcttgaaca tttgcagcat tcgcttcctt gcgtaatcgg tcggcaatgc
     2221 tcgggtcaat ctcaggcgcc acacggtgcg cctttcttgc cgcccagcgg cgcgcaagca
     2281 atagagcggc gatgactccg ataatcagga cagtcagcgg aagccaccag agaagtcgat
     2341 taaaaccggt gttagtgggc gccagcagca cccactcgcc gtattttgag acaaagaaat
     2401 ccttgatctc ctgcggagac ttgccggctt gcagttgctc gcgaacactg ccccgcatct
     2461 gttgggccat ttcggatggg gagtcagcca ccgaaaggtt ttggcagacc acgcagcgca
     2521 gttccgccga aatggcgcga gtctgctcct cgatgtcagg cgctggtgcg gcttggacgc
     2581 cgccgcttaa aagcccaagt aataaaatga gaataatgcg aaaagccatt gttctaacgt
     2641 acggattgaa attcgcctct tccttcttta gcgctaacga gaccctgctc cgcttcaacc
     2701 aacttctcag tcactacggc ggcccggata ccgccaacgt gtttataggt gatgcgtcct
     2761 tgaggatcga taaaaaagct ttccggaatg ccccaaaccc cgtaatcaat ggcgatcctg
     2821 ccggactcgt ccttgccgtt tggatacgtc acgccgaact ctttgagaaa ttcaagtgac
     2881 gcctgatcgg tgtcctggag cgcaactccg ataaaaatca cgttcttgtc tttaagcttt
     2941 tgccaagctg cttccagatc tcgcgcctcg gcgcggcaag gcgggcacca cgacgcccag
     3001 aaattaagaa atatcgcttt gccacgcaaa tcttcaaggc ggagtttact gccgttgaaa
     3061 agctcgatgg aaaacggcgt ggctggtttg gccagcaacg gcgacgtaat gtaacgcgca
     3121 tcacggctaa agccgaacgc cagtaacgcc aaaatgggcg caatgatcag taaagtaaac
     3181 gcgactcggc gccagggcat gctaaccggc ctcgttgcac gtgcaaaggc aaagcgcttg
     3241 cagaaaggtt ttcactgttc gaaagtgctc ggacatggca ttcgttcggt tcaggtgtcg
     3301 ttcacggact cttgtctctt atgggccgct cgctccggag tgcgagtctt ggcctggaaa
     3361 aaatagctcc taacaattaa gcaaacttca gaacatgacg ccagggtcat gtctcactcg
     3421 cggatggttt gcacatcggg cagatcgtaa ccagcgtccc gaacgcgatc acgaaaccgc
     3481 cgatccacat ccagacgacc aacggccgca ccagcacctt caaggtcgcc tgatttttat
     3541 ccagatcgct aaaaccggat aggatcaaat agatatcctc gctagggcta ctgcgatgta
     3601 ccgcccgtcc aatgggcgat tgctgggtcg ggaaaaactt gagagcgggg gataacagtc
     3661 cgatatcgga gccctgcttc agcacgcgaa aggcgccttc aacacgatag tgcgtcggtt
     3721 gctgcgagcc gtgcagccct tcaaagcgaa tctgataatt gccgatcgaa aggctttcat
     3781 taagcccgag cgtcgcctcg cgttcggtgc tgtaggtcat ggaaccggca atgcccataa
     3841 tgatcaacac gacacccaaa tgtacgacta agcctccata gcgtcgttga ttgcgccgtg
     3901 taagggtaat caggccgctg atgatgcctt cgttggcaag acggcgccgc gcccgcagcg
     3961 cgagagcggt gtcataaaca atggtcatga caacgaagcc gagcaaggta aagcccagca
     4021 gcggaaggaa atccttgacc ttccagacga acaaaaacaa accgaaaatc agcgatccaa
     4081 tggccggcca taggaaattc cgccttaaat tgtcccatga agctcgccgc caggcgatga
     4141 gcggaccgac gcccatgaga aacaccataa agagaaagag agggaccgta actgaattga
     4201 aataaggcgc gccgacgctg acctgcaccc cggcgactgc ttcggatagg agcggaaaga
     4261 tggtcccaag aaaaatcgta aagagagccg ataccagcac gatgttgttt agcaggaacg
     4321 ccgattctcg gctgaccatc gagtccaatt caggctgctc tttcaacata tcggagcggt
     4381 aggcgagcaa cccgaacgaa gctagtaaaa cgaaggcgag aaaaccaaga aaaaaaattc
     4441 caaccggtcc ggaagagaac gcgtgaatcg acgagagaat tccggagcgg gtgagaaatg
     4501 ttccgaagat tgtcaggcta aaggcgatga tgatcagcga aagattccac actttcagca
     4561 tgcgacggcg ctcctgcacc tggatcgagt gtaagaatgc ggtggccggc agccagggca
     4621 tgaacgcggc attttcgact gggtcccagg cccaatagcc gccccacccc aacacatgat
     4681 aggaccacca tgcgccgacc aggtttccgg cggtcagaaa gaaccacgcg atgatggtcc
     4741 agcggcgggt tgtagtgatc catgcttcgt ccaatttacc gacaatgagg gccgctatag
     4801 caaacgcgaa aggcaccgtc aggccgacga agccggtata gagcagtggc gggtgagtca
     4861 tcatgttggc gtcttcaagg agcggattca aacctcgccc atccgccggc acgggagaca
     4921 gagtttcgaa cggattcgaa acaaaggcga tgacggcaag aaagaaaaac gacacgatcg
     4981 agaagaccat gagcacccat gggagggttt ccttgtgacg gtcgcgatag atccacgcaa
     5041 tcagcccgga aaatataatc aagatccatt cccacaggag cagagaacct tccagagcgc
     5101 cccatagacc cgtcacgcgg taatagatcg gcgtggcgag agtggtatta aacgcgacgt
     5161 aacggaccga gaaatccgcc gacaccagcg cgaagatcaa tgccgatgcc gccagggtca
     5221 cgaaaatgaa ttgcccggca atggcgcggc caatgacaga cgaatacctc gcgtgaccgc
     5281 gacgcacgga aagcaccgga gcgacgattc cagccccggc ggcaagcaga gcgatcaaga
     5341 tgctgatgta gccgatggtt gcggtcactt cgaggtgtat tccttggcgg ggacgaagct
     5401 gtttgacgtg gcttgactgt cctcgtgcgg gctgtactct tcggcatgct tggccatgat
     5461 ggttgaggct tgaaacactc cgtccttgcc gatacggcct tcgaccactg cgcctgttcc
     5521 ctctctgaag aggtccggcg gcacgccacg aaaaaagacc gggaaggtac tcgagccgtc
     5581 ggtgagttcg aactcgtagg tgaggttctt cacgtctttc tgcagtgagc cctttacgac
     5641 catgccgccc atccgcaaaa acttatccat ggaggatgtt tccttcgcct taagttcgga
     5701 gggagtgacg aaataaacca gtgcctgctg catgccgccg tatatcacat atgagagcgc
     5761 tcccagtata atgatcccgc cgatcagaaa acgtctattt cttgccatga tcagaagact
     5821 cctcaattgc tcgcagttgg atcagctcct cttcggcgca ccggcaccgg cgcttcagcg
     5881 caaacaggta agtcacaatg cagccccaga caatgccgta agctagaaag acgaatcccc
     5941 aggaccccac ctaaaaggct ccttggttaa gccggcgagc cttgatttcg cttcgtactt
     6001 cgccgagtcg caggcgaagc gaaagaagat aaaaataaag cagaagaaat ccgacaaaat
     6061 tgatcgctag cgcggtcagc attgccggat gaacattctc ccatggaagc ttatcaggac
     6121 ggaggatcga cggcggctga tgcagggtgc gccaccagta caccgacatg tgaatcaatg
     6181 gaatgtcgag aaacccgatg attccaagta tggcggcgga caatgcggca cgatcttcat
     6241 cgtcgatcag cgagcggagc atcagatatc cgctatagat caagagcaga atggccgtta
     6301 acgtcagtcg ggcatcccag gtccaccata cgccccatgt cggcttgccc cagatcgatc
     6361 cttcgatgat ggtcaatgcg gtgaaaagga cgcccagctc ggcggcggaa tgagctaagt
     6421 tgtcgtcacg cttttcacgt ttccataagt agaggcagct accgacaaaa accagaaaga
     6481 aggaaagata agccgtgaga atactcggca aatgaagata catgatgcgc tggacatcgc
     6541 cctgaagagc atcccggggc gcctggacaa gcgcccagta aagcccgagc gcaatgatga
     6601 tcagcgcaag ggagccgaga acccccgagc tccttttttt gggctgctct ttcattagat
     6661 ttcgataacc cagtcgaaca ccaacactcc caaggtcaga aatatcacat caaatccgat
     6721 aagaagacgc aaccacggaa gcgcatcgcc gatctcgccg tcccgaagca cggttcccaa
     6781 gcactggatg gttgccaaaa tgaccgggat catcagcgga aagagcacca agggaagcaa
     6841 cagttcgcgc gcgcgggcgc gcaaggttaa cgccgacatg acggtgccga gcacgcaaaa
     6901 accgacagtt
//
LOCUS       DASUTG010000535         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_379439, whole genome shotgun sequence.
ACCESSION   DASUTG010000535 DASUTG010000000
VERSION     DASUTG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF089_12295"
     CDS             <1..195
                     /locus_tag="VF089_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6174788.1"
                     /translation="RGVIKWEQLIEIGAILAGQDDGRTRADQITLYKNNAGQGVADVA
                     LGALVLEKAKKKLAGQKLEV"
     gene            293..1111
                     /locus_tag="VF089_12300"
     CDS             293..1111
                     /locus_tag="VF089_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6174789.1"
                     /translation="MSRVAIVQIQSSTEKQKNMRRALELVKDSKSAGADLVVFPEFLM
                     AFSPLEQTAKELAELAEPLGGPFITSLKEAASATHTAIVATIYETCSIADRVYDTAVW
                     IDTEGNIAAAYRKLHLYDAFGFRESDKFAAGVDLTSPVTFEDGRYGMMICYDLRFPEM
                     ARMLTLSGANILLAPSGWVQGDLKLDHWQTMIHARALENGCYVIAPDQVGNIYIGHSM
                     IVDPLGRTVVDLGDREGMEIVEIDSNLVQDTRAKLPLLQNRRIDIYRNYLSSSG"
     gene            complement(1146..2087)
                     /locus_tag="VF089_12305"
     CDS             complement(1146..2087)
                     /locus_tag="VF089_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6174790.1"
                     /translation="MADRYLITGGAGFIGSHLVKHVLGTRGIVRVVDNLSTGSAEQLK
                     QVGDSAQLLTGDLAEFSVAAEAVKDIDYVLHQAAVPSVQRSIVDPAGTNRSNITATLN
                     LLENSRKAGVRRFVYAASSSAYGDTEVLPKNEEMPANPLSPYALQKWVGERYCKLYHE
                     LYGLETVSLRYFNVFGPGQDPYSEYSAVIPKFTTKLLAKEPITVYGDGEQSRDFTYID
                     NVIEANLLALRAPNAPGEVCNIGCGRRVTLNQLIRILEELLGVRAQITYAPAKPGDVR
                     HSLADISKATRVLGYVPKIDIEEGLRRTVEAFVKTND"
BASE COUNT          537 a          564 c          610 g          518 t
ORIGIN      
        1 cgcggcgtga ttaaatggga acaattgatt gaaattggcg ccatcctcgc cggccaggat
       61 gatggtcgca cccgtgccga tcagatcact ttgtacaaga acaatgccgg ccaaggcgta
      121 gccgatgtgg ctttaggagc gctggtttta gagaaagcaa agaaaaaact tgcagggcaa
      181 aagcttgagg tgtagaggcg gagcgagggg atgagtagct ggggtatttc gaacgagcga
      241 tgaccgctag atcctgatta tctcagaaag attggcgcta acgagtttct atatgagcag
      301 agtggcgatc gtccagattc aatcttcaac ggaaaagcag aaaaatatgc ggcgagctct
      361 cgagctggtc aaagatagta agagcgccgg cgccgatctg gtggtctttc ccgaattttt
      421 gatggcgttc tcgccgctcg agcaaacagc taaggagctc gccgaacttg ccgagccgct
      481 tggaggcccg ttcataacgt cgctgaagga agcggctagc gcgacgcata cagcgattgt
      541 ggcgacaatc tacgagacct gctccattgc cgatcgcgtg tacgacaccg cagtgtggat
      601 cgataccgaa ggcaatatcg cggctgctta ccgcaagctc cacttgtacg acgccttcgg
      661 ttttcgcgaa tccgacaaat ttgctgccgg ggtggatttg acatcgccgg tgacgttcga
      721 agatggccgc tacggtatga tgatttgcta tgatcttcgt ttccctgaga tggcacgcat
      781 gctgactcta tcaggagcca acatactgct ggcgccttct ggttgggtcc aaggcgattt
      841 gaaacttgat cattggcaaa cgatgataca tgcgcgtgct ctcgagaacg gctgctacgt
      901 cattgcgccg gaccaggttg gtaatattta cattggtcat agtatgatcg tcgatccact
      961 agggcgcact gtagttgatc tcggcgaccg cgaaggaatg gaaatcgttg agatcgattc
     1021 taatttagtc caagacaccc gcgccaagtt gccgctcctg cagaatcgca gaatcgacat
     1081 ctaccggaat tatctgtcct cgtccgggtg agaagatctc cgagtgatgt cgatgctcgt
     1141 ccgcatcaat cattcgtttt tacgaacgct tcgaccgttc tccttagtcc ttcctctatg
     1201 tcgattttcg gaacgtatcc gagaacccgc gtggccttgg aaatatcggc caacgagtga
     1261 cgcacatcgc ccggcttagc gggagcgtaa gtgatctgtg cccgaactcc gagaagttct
     1321 tccaaaatcc ggatcagctg attaagggtc acgcgccggc cgcaaccgat gttgcaaact
     1381 tcccccggtg cgttcggcgc acgcaatgca agcaggttcg cttcgataac attatcgatg
     1441 taggtgaaat ctcgcgactg ctcaccgtct ccatagacgg tgataggctc tttagctaaa
     1501 agcttggtag taaatttagg aatcacagcc gagtattctg agtaagggtc ttgcccggga
     1561 ccgaaaacgt tgaagtacct gaggctgaca gtctcgaggc cataaagctc atgatagagt
     1621 ttgcagtagc gctcccctac ccatttctgt aacgcgtagg gagaaagtgg attcgccggc
     1681 atttcttcgt ttttcggcag aacctcagta tcgccgtaag ctgaagacga agcggcgtag
     1741 acgaagcggc gaactcccgc cttccgcgaa ttttccagga gatttagagt tgctgtgata
     1801 ttggaccggt tggtaccggc tggatcaaca atcgatcgct gtacggatgg aacggcggcc
     1861 tgatgtagaa catagtcgat atctttgaca gcttctgcag caacggaaaa ttccgccaag
     1921 tcgccagtca agagttgcgc cgagtcaccg acctgtttga gttgctcagc ggagccagta
     1981 gacagattat ccacgacccg aacgatgccc cgggtaccga gaacatgctt tactagatga
     2041 gacccaataa accccgcacc gccagtgatg agataacgat ctgccatgcc ttacacgccc
     2101 cttttctctg tctccgccga atcgcagagg tattgtacga cgggaattat agcaggcata
     2161 actgtgcatg catatgagat gatgcgcctt gcctgatcgc accgatacta agtcggcgag
     2221 ctgcggaat
//
LOCUS       DASUTG010000536         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_973313, whole genome shotgun sequence.
ACCESSION   DASUTG010000536 DASUTG010000000
VERSION     DASUTG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973313"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="holB"
                     /locus_tag="VF089_12310"
     CDS             complement(<1..659)
                     /gene="holB"
                     /locus_tag="VF089_12310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX6174791.1"
                     /translation="MGFSEIVGHEKQLETLRQSLLNGRLHHAYLFIGPDGIGKRTLAL
                     ALAQAIHCAELEGDFCGECGACRAIQTGNHADVRVLEPLSNKKDISIQQVRELEKTLS
                     FRSFSGRRKMAIIDPATSMNWPAQNALLKTLEEPPQGCILILLASNAGGLLPTVRSRV
                     FALSFTPLPRQLMISFLVSRRGRTREQAEILAAVTMGSLGAVSRFDKEKMIEKRLDWM
                     KT"
     gene            complement(678..1325)
                     /gene="tmk"
                     /locus_tag="VF089_12315"
     CDS             complement(678..1325)
                     /gene="tmk"
                     /locus_tag="VF089_12315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX6174792.1"
                     /translation="MVGFITFEGGDGTGKSTQIRLLERHLIAQGKSCLTTREPGGTSL
                     GQLLRQILLQSGNRAISSSTELFLYLADRAQHVHEVIAPAIAQGKIVLSDRHTDSTVA
                     YQGYGRGFDLQLLLSLNRIASEGLTPDLTFLLDCPVELGLSRTVHRRSGSATVKSNDR
                     FENENLEFHEKVRQGFLDLARAEPARFYIIDASHSEQEVFAAVREIVDRRLDLSS"
     gene            complement(1413..1796)
                     /locus_tag="VF089_12320"
     CDS             complement(1413..1796)
                     /locus_tag="VF089_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6174793.1"
                     /translation="MDTQRTIMVVDDNPDIITIVTTILEGKGYQVLSASSGQELLNLL
                     TDRKPDLIILDIMMPEMDGLEVLGRLKAVTETASIPVILLTAKVQYEDVLGGYKLGAD
                     YYITKPFTSTQLVNGINLLLGESKN"
     gene            complement(2014..3198)
                     /locus_tag="VF089_12325"
     CDS             complement(2014..3198)
                     /locus_tag="VF089_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEX6174794.1"
                     /translation="MQVITYSRTLIADERGIVLYSSLMLLALIMAVGVQAIVSTQSNF
                     QISSNLRSENVAFYISEAGIEWSKNELARTIAHPPPALSYSSAISSGMFYVATISTVA
                     ESPLISNTVIRSTGKFASSSQIIQAELIKRSLLADAALGLRGNASHPMFVGNDFSISG
                     LDHDPATGKPIPNVRPYAGISVSNAAIKEQIETALEPDQLSRISGTDRQDATIAETEN
                     LPTEAIVRLADDLCSAENSQFTEVAADTTLSLADQQWGNRSIPELHCLSGTLGAGDSV
                     AALSNSSGAGILVARNADLVLEGGFRWEGLIIITGSRASLKISDAGIKEIIGGLIINE
                     TGPHSESNPPTLDVRGASKILFSRAALQSAASVVPASALNTAYTSLPAEIVQSYWRLI
                     TP"
     gene            complement(3198..3845)
                     /locus_tag="VF089_12330"
     CDS             complement(3198..3845)
                     /locus_tag="VF089_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174795.1"
                     /translation="MQITQNDCGATLIEQLAALLLGTIIITALFSFYRTELFHLLAQE
                     TRMATLEDARGAMDIISRDLKLAGSWGTGSAPPETGLGDDPDGDEDSACNRIYRATER
                     VIQIQMDLNGNGNCADLEPRENIRYELGAPTSTCPGATVIRRNGDCLVAHAIAPNSGK
                     LFRYYDENGSQLSEAPPLSAIKRIGISFSVGMKQTDSKTGASLTSALSNSVDLRN"
     gene            complement(3854..4264)
                     /locus_tag="VF089_12335"
     CDS             complement(3854..4264)
                     /locus_tag="VF089_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174796.1"
                     /translation="MLPGFVAPKQSQGGFTFNEVLLAMNVVVIGILGYSMGTGNLIRW
                     NSASRDYTVAVNLAQDKLEEIKAQKNWTDVNNCPTAGDLNIAANGEAGGQYNRCWIIA
                     DSALGAELKEIMVTVSWPGRESREVTLTTLIYSG"
     gene            complement(4267..4713)
                     /locus_tag="VF089_12340"
     CDS             complement(4267..4713)
                     /locus_tag="VF089_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6174797.1"
                     /translation="MDCQSRVGFSVIELIVVLSLMGILLAIGIPASNKLLLSYQLNGS
                     ARRVQTELQNIKMRAAAENLGFQFAYGAGSGSYTIRKDGSVFVSKPLPEGIVIMQAGT
                     VSFSPRGTAGSNRIRLQSREGGCKQVVVSSTGRIRVCNPAGCTEDC"
     gene            complement(4883..5680)
                     /locus_tag="VF089_12345"
     CDS             complement(4883..5680)
                     /locus_tag="VF089_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX6174798.1"
                     /translation="MPNFYIGFIVFIFGAVVGSFLNVCISRIPKGESVIHPPSHCPKC
                     QTPIPFFYNVPLLSYLLLRGRCCSCGERIAFRYFAVELLMASLAVLLLYQFGLGLAFV
                     VGFVFVAALIVISFIDLDVRIVPDVISLPGIVIGLVFSVIGHYFIRDSLDVVPTPLGA
                     LAGVLAGGGFLLAVAWIYEKFTGIEGMGGGDIKLLAMIGAFLGWPSIPLTLFFSSLGG
                     SIIGLSIMLVKGVDRRYALPFAPFLCLGALVYLFFGREIIDFYVFSR"
BASE COUNT         1521 a         1666 c         1575 g         1431 t
ORIGIN      
        1 gtcttcatcc agtcgagcct cttttcgatc atcttttctt tatcgaacct gcttaccgct
       61 cccaaacttc ccatcgtcac agccgcgagg atttcggcct gctctctcgt cctacctctg
      121 cgggagacta agaaactgat catcagctgc cgtggcaatg gcgtgaaaga aagggcgaaa
      181 acgcgcgagc gcacagtggg gagcaggccc ccagcgttag acgcgagcag aattaaaatg
      241 cagccctggg gcggctcttc cagcgtcttc agcaatgcat tctgtgcagg ccagttcatc
      301 gaagtcgcgg gatcgatgat cgccattttc cggcgaccgg aaaaagatcg gaaactcagc
      361 gttttctcta gctcgcgcac ctgctgaatg ctgatatctt tcttgttgga tagaggttcc
      421 aagacgcgga catcggcatg attgcctgtt tgaatggccc gacaggcacc gcactcgccg
      481 caaaagtcac cctccaactc cgcgcagtga atcgcctgag ccaacgccag cgcaagagtt
      541 ctcttgccaa taccatcggg cccgataaat agataggcgt gatgcaatcg tccgttcagg
      601 agggattgac gcaaggtttc taattgtttt tcatgaccaa cgatttcaga aaagcccatg
      661 attggttcta ctagttttta gctcgacaaa tccagccttc ggtcaacaat ctctctaacc
      721 gctgcgaata cttcttgctc ggaatgcgaa gcgtcgatga tataaaaccg cgcgggttcc
      781 gctcgcgcta aatcgagaaa cccctggcgc actttttcgt gaaattctaa gttttcattt
      841 tcaaaccgat cgttactttt aaccgtcgcc gaccccgacc gacgatgcac cgtacgcgac
      901 aaccctagct ctacgggaca gtcgagcaaa aacgtgagat cgggagtcag accttcgctg
      961 gcgatccgat tcaggctcag cagcaattga agatcaaaac cccgtccgta gccctggtac
     1021 gccacagtcg agtcggtgtg tcggtcagac aaaacaatct taccctgcgc gatcgcgggc
     1081 gcgattactt catggacatg ctgggcgcgg tcggccaaat acaaaaagag ctcggtcgat
     1141 gagctgatcg cccgattgcc gctttgtagg agaatctggc gcaggagttg acctagcgaa
     1201 gtgcccccgg gttctcgcgt cgtcaggcag gatttacctt gcgcgatcag gtgccgttcc
     1261 agcaatctaa tctgcgtaga tttaccggtg ccgtcaccgc cttcaaaagt gatgaaacca
     1321 accatgaatt tgatcaacag aggctgtttg cgtgtacctt tgtaccacgc cggtgcggcc
     1381 gtagaaatgg ctgaggtgag attgagccgg ggctagttct tgctttcgcc taggagcaga
     1441 ttgatcccat tgactaactg tgtgctcgtg aaaggcttcg taatgtagta atcggcgcct
     1501 agcttatacc cgcctagaac atcttcgtac tgcaccttgg cggttaacag aattacagga
     1561 atggaagcgg tctctgtgac ggcctttagc cgacccagaa cttccaatcc gtccatttca
     1621 ggcatcatga tgtctaggat gatcaggtcc ggctttcgat ccgtcaaaag attcaataac
     1681 tcttggccgc ttgaagcact cagcacttgg tagccctttc cttccagaat ggttgtaacg
     1741 atagttatga tatctgggtt atcgtcaact accattatgg ttcgctgtgt gtccatgcgt
     1801 tctactccta gagctgcaag gttcgaggat cgagattgag caatttacca ccttatcccg
     1861 gtaatgtaaa ttattgtttt ggttatctat tacggccgct ttcgcagatt tgcggctaca
     1921 tgcgaagact tcagttggcg acactcacag cgcgggccaa gatgatcatg agaggttgga
     1981 tttaagcagc ggagaatcgg tcggaattta cttctaaggc gttatcagtc tccaataact
     2041 ctgaacgatt tctgcaggca atgatgtgta agcggtgttc aaggcgctgg cgggcacgac
     2101 gcttgcggca ctttgtagag cggcacggct aaaaagtatt ttggaagcgc cacgaacatc
     2161 aagtgttggc ggattactct cggaatgagg accagtctca ttgatgatca agccaccgat
     2221 aatctctttg ataccagcgt cggatatttt taagcttgcc ctgctgcccg tgatgataat
     2281 tagcccctcc catcggaacc caccttccaa tactaaatca gcatttctag cgaccaggat
     2341 cccggcacct gacgaattgc ttagagccgc aactgaatcg ccggcaccga gggtgccgct
     2401 taggcagtga agctcaggga tagatcggtt gccccactgt tgatcggcca acgatagggt
     2461 agtatcggcg gcaacctccg tgaattggct gttttctgct gaacacagat catccgcaag
     2521 tcgcacaatc gcttccgttg gcagattctc ggtttcagca attgttgcgt cctggcgatc
     2581 agtgccggaa attctgctga gttgatcggg ttccagcgcc gtctcgattt gttccttgat
     2641 cgcggcattt gagacgctga ttccggcata cggccggaca tttgggatcg gcttacctgt
     2701 tgctggatca tgatccagcc cagagatcga gaaatcgttg ccgacgaaca tcgggtgact
     2761 tgcgttgccg cgcaatccga gggcggcgtc ggccagtaga gaacgcttga tcagttcggc
     2821 ttggatgatc tgcgaggatg aggcaaactt gccggttgat cgtatgacgg tgttggatat
     2881 taacggcgac tcggcgaccg tagaaatggt cgccacataa aacatcccgg aggagattgc
     2941 cgaggaatag cttagtgccg gcggcggatg agcaatcgtt cttgcgagtt catttttgct
     3001 ccactcgatc cctgcttccg atatatagaa ggcaacgttt tccgaacgca aattcgatga
     3061 tatctgaaag ttgctttgcg tcgacacgat cgcctgcacg cccacagcca tgatcagggc
     3121 caacaacatc aatgaagagt aaaggacaat cccacgctcg tccgcaatca gagttctgct
     3181 gtaggtaatg acttgcatta gttccgcagg tcgacgctgt tagaaagcgc cgaggtgagg
     3241 cttgcgcccg ttttggaatc cgtttgcttc ataccgacag agaacgaaat cccaattcgc
     3301 ttgatggctg atagcggcgg tgcttcgcta agctgtgaac cgttctcatc gtagtaccta
     3361 aacagcttgc ctgagtttgg cgcgattgca tgagcgacca ggcagtcacc attgcgtcga
     3421 atgaccgttg ctccagggca ggtcgaagtt ggcgcgccaa gctcgtagcg gatgttttcg
     3481 cgcggctcaa gatcggcgca attgccgttt ccattgagat ccatttgaat ctggatcacc
     3541 cgctcggtcg cgcgataaat gcgattacac gcggagtcct catcaccgtc cggatcgtct
     3601 cccaggccgg tttccggagg cgcgcttccc gtgccccatg aacctgcgag tttcaaatcg
     3661 cggctaatga tatccatcgc ccctctggca tcctccaggg ttgccatccg cgtttcttgt
     3721 gcaagaagat ggaacagttc ggtccgataa aatgaaaata gagctgtaat tattatcgtc
     3781 cccagcaata gcgcagccaa ttgctcgatc aaagtggcgc cgcagtcgtt ctgagtaatt
     3841 tgcataggca atctcaacct gaataaatga gcgtggtgag agtcacttct cggctttcac
     3901 gaccgggcca cgaaaccgtc accatgatct ccttcaactc cgcccccaat gccgaatccg
     3961 caattatcca acagcgatta tactgaccgc ccgcttcacc gttagcagcg atattgaggt
     4021 cgcccgctgt ggggcagttg ttcacgtcgg tccaattctt ctgcgctttg atctcctcga
     4081 gcttgtcctg cgccaggtta accgcaacgg tgtaatcgcg gctggcggaa ttccaccgga
     4141 tcaggttacc cgtgcccatc gaatatccga gaatgccgat gacaacaacg ttcatcgcca
     4201 gcagtacttc gttgaacgtg aatccgcctt ggctttgctt tggcgcaacg aatcctggca
     4261 acattctcag caatcctcgg tgcaaccggc aggattgcag actcgaatcc tcccggtgga
     4321 actgaccaca acttgtttgc atccgccttc tctactttgc agtcgaattc gattgctgcc
     4381 tgcggtgcct ctcggggaaa aggaaacggt gccggcttgc atgattacta tgccttcggg
     4441 taatggcttg ctgacaaaca cgctgccgtc cttgcggata gtgtagctgc cgctgcctgc
     4501 gccgtatgcg aactgaaaac ccaggttctc cgctgccgcg cgcatcttga tgttctgtag
     4561 ctcggtctga acgcgccgcg cagaaccgtt caattggtag gaaagaagta acttgtttga
     4621 ggctggaatc ccgatcgcaa gcaagatccc cattagactt agcaccacga taagttcgat
     4681 aacagagaat ccgaccctgc tttggcaatc catggctcct ccagaagttt gcggaccata
     4741 taggtgcgcg tgcggggctg tcaagagccg caagccaggt tgatcgaagc taaatcgctc
     4801 agcttatggc gaaactaggc aggagggcgg caactacgac gcccgcaggc ttactttaga
     4861 attgggataa gagaagtgag gctcaacgcg agaagacgta gaaatcaatg atctctctgc
     4921 cgaaaaacaa gtacacaagt gcacccaaac agaggaaagg agcaaacgga agagcgtatc
     4981 ggcgatctac tcctttgacc aacatgatac ttaggccaat gatagaacct cctaaggatg
     5041 aaaagaacaa ggtcaacgga atcgaaggcc acccgagaaa agcgccgatc atggcaagga
     5101 gcttaatatc gccgccaccc attccctcta tgccggtaaa cttctcataa atccaagcta
     5161 cagccaaaag aaatccgccg cccgccaaca cgcccgccaa cgcgccgaga ggagtaggca
     5221 caacatccag cgaatcacgg atgaaataat ggccgatcac cgagaacaca agcccaatga
     5281 caatgcccgg caaactgatc acgtcgggaa caatcctgac atccaaatca ataaaagaga
     5341 taacaatcag cgccgcaacg aaaacgaacc ccacaacaaa agcgagccct aagccgaact
     5401 gataaaggag aagcaccgcc agagatgcca tcagtaattc gacggcgaaa taacgaaacg
     5461 caatccgctc cccgcaggaa cagcagcggc cgcgtaacag caaatagctg agtaaaggaa
     5521 cattgtaaaa gaatggtatc ggtgtttgac acttggggca gtgcgatggc ggatgaatga
     5581 cggattcgcc tttgggtatt cgggaaatgc agacgttcaa gaagctgccg acgacggcgc
     5641 cgaagatgaa aacgataaag ccgatataga aatttggcat cagatgattg gtccttcttt
     5701 tgcctgagag atcaacatcc tccactgcgg cggaagatct caggcgtgct gctactaaag
     5761 atcatcacgg aggttctttt cgaatagtca gtcgatagtt ctctaagcac tacctccatg
     5821 tcagccccaa ccagacgcct gaaccggatt ccatttgcac cgagtacaga tcgtttaact
     5881 tttccgcgtc ttccatatgc gtcagatcga attctcagct ctatcggcgc aaggtcctgt
     5941 gacggcgcga actgcgagtc accactgagc ggtcctccaa cgtcaattga gggtcttact
     6001 caccttcgtt catacgagga cgctgaattc aacaaagtcg cagtttagga agctcggata
     6061 accttattca ggatcttcta acaaccatgc tgcatccggg ccctacatcg acgaaccctc
     6121 ccgaacaccc gagcagcgtg tttcttccaa aacagaggct gagcaaccga gtcgcttttg
     6181 tagatgtgcc ttt
//
LOCUS       DASUTG010000537         5569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_379791, whole genome shotgun sequence.
ACCESSION   DASUTG010000537 DASUTG010000000
VERSION     DASUTG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379791"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1299
                     /locus_tag="VF089_12350"
     CDS             163..1299
                     /locus_tag="VF089_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174799.1"
                     /translation="MSKAAQADRKMWSKIYTYDEWMEAQGIPIYRGYYIEDLRTLELG
                     WWEARECKAAFIQLVGQEGVTSARVTEISPGKTLSPLKFSLDEIVYVVEGRGLTTIWA
                     GENRPKKTFEWQKHSMFLLPHNYTHQFSNMQGDKPARLLQYNYMPLTLSGVRDVNFIF
                     GNPYEGPDIQNELDFYSEAKMVQQNLLDDSWGRRAYWYGNFFPDMRAWDKLDSNARRG
                     AGGRSVYIQFPDSEMSCHMSVFDARLYKKAHRHGPGRVIVIPVGEGYSIMWEEGKEKV
                     VVPWHEASCFVPPNRWFHQHFNAGGEPARYLALHPPMQFHGHAEKVEDRAKDQIEYVN
                     EDRWVREKFEAELTKRGITSLMPPEAYVDPDFEWNWSPFLRKSS"
     gene            complement(1317..2219)
                     /locus_tag="VF089_12355"
     CDS             complement(1317..2219)
                     /locus_tag="VF089_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6174800.1"
                     /translation="MGKIGLAVVSPAPLITPGYVLNFAKKCEAMGADSMWTIDRVAYD
                     NLEPLTMLATAAGATQRLRLGTSVLLGNLRHPTHLAKIVATLDFISNGRVTLGLGFGS
                     RESDYKAVEVPFDKRGSRAVEQVDLIKRLWTEENVTYNGRFFNVENLTLGPRPIQKSI
                     PIWTGGSAEVALKRAGTWADGYICGSSAIPEFPQTWEKIAGYARSAGRDPEVIKKAGL
                     TFMAIDDDQGRAVKTIENYMTRYYGKVRGEVASTSLVGSPSAITERIDSFLSRGLDTL
                     IVGLTDPDPRQLDLFGDKILPKLN"
     gene            2342..2776
                     /locus_tag="VF089_12360"
     CDS             2342..2776
                     /locus_tag="VF089_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174801.1"
                     /translation="MEAATFKLKTPHITGGRSHTPLAQTEHMTIGLNYYVPGRKNKLH
                     THPGEDHTFVVMDGQATFYNKDHQPTVLKKGEGIILPENHYYYFQSTGDRPLALFRVS
                     AKKGTKPKVLRVDSEGNKRTDEEIDFVVVDGETVEGKFWELT"
     gene            2784..3938
                     /locus_tag="VF089_12365"
     CDS             2784..3938
                     /locus_tag="VF089_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6174802.1"
                     /translation="MKITDIETLRLRAPIPQDGQVFSRSGVRSYRSTTLVRIDTDEGI
                     SGWGSCSGNGELIEFVVARILRPLIIGMDPTRIDDVWDQAYVRGGHKEFGTRGIGVVA
                     LSGIDVALWDIVGKLQGQPLYQLLGGKCRDKVPVYATALYPEKPSTVAKRARVFANQG
                     FHGVKIKVGFDLDQDIEIVRAVRQELGRDFIVMTDANQGYTMGVGLKAAEAFADCGVF
                     WLEEPLFADDIAGHARLREATGVPIAVGENLQMCSAFENFVVREAVDFLQPDVARAGG
                     ITEINKIAALAARHGRPISFHTWGDAVALAASVHLSAALPDCTVMEIDYTYNPLRAEL
                     LRQPLEVESGFMVPPDKPGLGIQIDADALERFAFSGVEELVVRQKALVAR"
     gene            4402..>5569
                     /gene="mnmE"
                     /locus_tag="VF089_12370"
     CDS             4402..>5569
                     /gene="mnmE"
                     /locus_tag="VF089_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX6174803.1"
                     /translation="MYQEDTIAAIATPIGEGGVAIVRVSGPDAERAVRALFVRPRQQD
                     TKLRSHMLYHGSIRDPHTRVTVDKVLITMMRRPHSYTGEDVAEIHCHGGAFLVQRILG
                     LVLAQGVRHAQRGEFTKRAFLNGRMDLSQAEAVLDLIRARTDKGLQLAVQNAEGVLSK
                     CVGDLREEVIGILVQVEAAIDFPEEDIELLKRAALTARIAALREKIESILSTYEWGRL
                     LREGARVCICGRPNVGKSSLLNALVGDSRVIVSAIPGTTRDVIEDSINLDGLPVVLWD
                     TAGIHETENEVEKTGVGLSLEHIEKADGVIAVLDGSESLTQEDVSVLLNIDQKKSLIA
                     INKSDLQQRLDLARAKNFVANACIVPVCAKDGTGVDALKANLRQVLLERSIESPV"
BASE COUNT         1318 a         1370 c         1571 g         1310 t
ORIGIN      
        1 gttgtccgct gcagccgcct gatagaaaca atccgcccgc aacacattct ccttcgtagg
       61 gtgtagcgta atcgccgtaa gtttctgcgc tcagcctggt gcaattttgt ttcgatttcc
      121 aaggatgata tgactcaatg agtcacctgc ggaggaaaag ccatgtctaa agcagcgcaa
      181 gcagatcgca aaatgtggag taagatctat acctacgacg agtggatgga ggcacaaggt
      241 attcctattt accgcggtta ctacatcgag gatttgcgca ctttggaatt ggggtggtgg
      301 gaagcgcggg agtgcaaagc ggcgttcatc cagctggtgg gacaggaagg cgtgacttca
      361 gctcgggtca ccgaaatttc accaggcaag acgctatcgc cgcttaaatt cagcctggat
      421 gaaatcgtct acgtggtcga aggccggggt ctgacaacga tttgggccgg ggagaatcgg
      481 ccaaagaaaa cctttgaatg gcaaaagcac agcatgttct tgttgccgca caattacacg
      541 catcagttca gcaatatgca gggagacaag ccggcgcgct tgctgcaata taattacatg
      601 ccactgacgc tatccggggt ccgcgacgtc aacttcattt tcggcaatcc ttacgaagga
      661 cctgatattc aaaatgagct ggatttttat tccgaggcca agatggtcca gcaaaacctt
      721 ctggatgact cttggggacg gcgcgcctat tggtatggca acttcttccc cgatatgcgc
      781 gcttgggaca agctcgatag caacgcccgg cgcggcgcag gcggccgaag cgtctacatc
      841 cagttccccg attctgagat gtcctgccat atgtcagtat tcgatgcgcg gctctacaag
      901 aaagcgcacc gccacggccc gggccgcgtg atcgtcatcc ccgtaggcga gggttattca
      961 attatgtggg aggaagggaa agagaaagtc gttgtgccct ggcatgaggc tagctgcttc
     1021 gtgccaccca acagatggtt tcatcagcat ttcaatgccg gcggcgagcc ggcgcgatat
     1081 cttgcgctgc atccacccat gcagtttcat ggccatgcgg aaaaggtcga agaccgcgcc
     1141 aaggatcaaa tcgagtatgt caatgaggac agatgggttc gcgagaagtt cgaagccgag
     1201 ttgaccaagc gcggcataac ttctttgatg ccgcccgaag catatgtcga tccagatttc
     1261 gagtggaatt ggtcgccttt cctgcgcaag tccagctaat cgagaactga attgcgctaa
     1321 ttgagcttcg gcaggatttt gtcgccaaac aaatccagtt gtctcgggtc tggatcggtc
     1381 aagccgacga tcagtgtgtc gagcccgcga gatagaaaag aatcgatgcg ttcagtaatc
     1441 gcgctcggag agcccactaa ggatgtgctt gcaacctctc ccctaacttt gccgtagtac
     1501 cgggtcatgt aattctcgat ggttttaacc gccctaccct ggtcgtcatc aatggccatg
     1561 aaagtgagcc ctgctttttt gataacttcc ggatcgcgcc cagccgagcg cgcgtaacca
     1621 gcgattttct cccaggtctg aggaaattcc ggaattgccg agctaccaca gatatagccg
     1681 tcggcccagg tgccggcgcg tttgagcgcg acctcagcgc tgccgccggt ccagattgga
     1741 attgacttct gaatcggccg tggaccgagg gtgagattct cgacattgaa aaaccggcca
     1801 ttgtaggtga cgttctcttc tgtccagaga cgtttgataa gatcgacttg ttctactgcc
     1861 ctactaccgc gcttgtcgaa aggcacttcc acagctttgt aatcgctctc ccgactgccg
     1921 aagccgagtc caagtgtcac ccgtccattc gaaataaaat ccaatgtggc aacgatcttt
     1981 gccagatgag tggggtgtcg caggttgccg agtagcactg aggtacccag acgaagtctt
     2041 tgtgtcgcgc cggctgcggt cgctaacatc gtcaacggct cgagattatc ataggcaact
     2101 cggtcgatgg tccacatcga atcagcaccc atggcctcgc actttttggc aaagttgaga
     2161 acataacctg gcgtgatcaa tggcgccgga ctgacaaccg caagaccgat cttacccatt
     2221 ctgttttctc cttttatgga tagaacaaca ctttgatgat gtcctactaa attagactag
     2281 gcagaacgcc cttttcaagc ttaattgtct gggatatacg agtcataagg aggagaccta
     2341 aatggaagct gctacattca agctcaagac cccgcacatc actggaggcc gtagtcatac
     2401 accgctggcg caaacagagc atatgaccat tggcctgaac tactatgtac cgggaaggaa
     2461 aaacaagctg catacgcatc cgggagagga ccatactttt gtcgtgatgg acgggcaggc
     2521 cactttctat aacaaggatc atcagccgac ggttctcaaa aagggtgaag gaatcattct
     2581 gccggagaac cattactact attttcaaag caccggtgac cggcctttgg cgttatttcg
     2641 ggtgagcgca aagaagggca ccaaacccaa ggttttgcgg gtggattccg agggaaataa
     2701 acgaaccgac gaagagattg actttgttgt tgtcgacggc gagaccgtag agggcaaatt
     2761 ttgggagcta acctaagcgc taaatgaaga tcactgacat cgaaaccctt cgtttgcgtg
     2821 cccctatccc gcaagacggg caggtcttca gtcgctccgg agtaagaagc tatcgttcga
     2881 cgactctggt ccggattgac actgacgaag ggatttccgg atggggttcg tgctcgggca
     2941 acggcgaact catcgaattc gtcgtggcga gaatattacg tccgctcata attggtatgg
     3001 acccgacccg gatcgatgat gtttgggacc aggcttatgt tcggggcggc cacaaagagt
     3061 tcggcacacg tggcattggg gtggtcgcgc tgagcggaat cgacgttgct ctgtgggata
     3121 ttgtgggaaa gcttcagggt cagccgctct accaactctt aggcggtaag tgccgggaca
     3181 aggttcctgt atacgccacc gctctttatc ctgaaaaacc gtcaacggtc gcgaaaagag
     3241 ctcgcgtttt tgctaaccag ggctttcacg gtgtcaagat taaggtcggt ttcgacctcg
     3301 atcaagacat cgaaattgtt cgcgcagtgc gccaagagtt gggcagagac ttcatcgtga
     3361 tgactgatgc gaatcaaggt tataccatgg gtgttggctt gaaagcggcc gaagcctttg
     3421 ccgactgtgg agtgttctgg ctagaggagc cgctatttgc ggatgacatc gccgggcatg
     3481 cgcggttgcg ggaagcaact ggggtgccga ttgccgtagg tgagaacttg caaatgtgct
     3541 cagccttcga gaatttcgtc gtgagagaag ctgtcgattt ccttcagcca gacgtggcgc
     3601 gcgctggcgg cataactgag ataaacaaaa tcgccgcgct cgctgcgcgc catggccggc
     3661 caatatcgtt tcatacctgg ggagatgcgg tggcgctagc ggctagcgtc catctttcag
     3721 cggccctgcc ggactgcacc gtgatggaga ttgactacac ttacaatcct cttcgggcgg
     3781 aactattgcg gcagccttta gaggtggaaa gcggcttcat ggttccccct gataaaccgg
     3841 gattgggaat ccagattgat gccgatgctt tggagcgttt cgcgttctct ggcgttgaag
     3901 agcttgtggt acggcagaaa gcgttagtcg cgagataatg cgttgtgggt acccggcgag
     3961 cagcgcgacc ttaacgcgaa cgcgagggct gggctaacat tgccgctctg ccgaggcaac
     4021 gtgttgctag aaacaggttt gcactcagca caatcgaaaa tcgcctgatc tgatcttccg
     4081 ctgagttcga tgcttttaag tggcttcttc cgaatgctgg cttcccatcc tccatcgata
     4141 tttaaagatt tgtgctatga agtccgcgct cgttggttag ttggttaaca gaacggcgct
     4201 ttctgaacgg gtttcctgca acgagttcaa agatcgtctt ctgtggtatc cgcgccaagg
     4261 gacccgccaa gaggctttgg ggcatctatt tagcggcgat ttagctgatc cgcgaacgaa
     4321 agtcactgtc cataaacctg cctcgtaatt ggcgctgttg ggttgccttg cgagtcgaag
     4381 ttgctacgat tggtaggctt tatgtaccag gaagatacga ttgcggcgat cgcgacaccg
     4441 atcggagaag gcggagtggc gattgtccgg gttagtggac ccgatgctga aagagctgtt
     4501 cgtgcgctgt ttgtgcgtcc acgtcagcag gacacgaaac tacgttcgca catgctctac
     4561 cacggctcaa ttcgagatcc gcatacccgt gtcacagtcg ataaggttct gataacaatg
     4621 atgaggcggc cccatagtta tacaggagaa gacgtcgccg aaattcattg tcatgggggt
     4681 gcctttttgg ttcaacggat tctcggcttg gtgctcgcgc aaggggtccg tcacgcgcaa
     4741 cgcggtgagt ttaccaagcg ggcgttcttg aatggtagaa tggatctttc acaggccgaa
     4801 gcagtgcttg atctgattcg cgcacgcacg gataagggcc tccaattagc ggtccaaaac
     4861 gccgaaggcg tactatccaa atgtgtgggc gatcttaggg aagaagtgat tggtattctt
     4921 gtccaggttg aagccgcgat tgactttcca gaggaagata ttgagctttt gaaacgtgct
     4981 gcattgacgg caagaatcgc tgccctgagg gaaaagattg agagcatttt aagcacttac
     5041 gaatggggaa gattgctcag agagggcgca cgagtatgta tttgcggacg tcctaatgtg
     5101 ggtaagtcga gccttctcaa tgccctcgtg ggcgatagcc gggtcatagt cagcgcgatt
     5161 cccggcacca ccagagatgt tattgaagac tctatcaatc tggacggctt acccgttgtg
     5221 ctatgggata ccgcagggat tcatgagacc gagaatgaag tggagaaaac cggcgtcggc
     5281 ttgtctctgg aacatattga aaaagcagat ggtgttattg ccgtattgga tggttccgag
     5341 tcgctgaccc aagaggacgt ttcagtcctg ctcaacatcg accaaaagaa gagtttgatc
     5401 gctataaaca aaagcgacct tcaacagcga ctcgaccttg ctagagcaaa gaattttgtt
     5461 gccaatgcat gcattgtccc tgtttgtgcc aaggatggca ctggagtgga cgcattgaaa
     5521 gctaacctcc ggcaggttct tctcgaaaga agtattgaat cgccggtga
//
LOCUS       DASUTG010000538         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2159676, whole genome shotgun sequence.
ACCESSION   DASUTG010000538 DASUTG010000000
VERSION     DASUTG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159676"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VF089_12375"
     CDS             complement(<1..958)
                     /locus_tag="VF089_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174804.1"
                     /translation="MPESPVRQSNLSPRPLMRILGDFANSQILDASIEYDFFTLIGKG
                     FHTADEIAGQAGTDPRATRIVLDSLLALALLEKQNGHYQLTPISEAFLVRDKPSYVGD
                     FRHVALALWDGMAHLKESLKTGKPFSRLDTGEALEVWEKLVLGIIVIAEPAARALCDL
                     LKIGTERKAINVLDIAGGSSIFGMTILSRDPSAKVTQLDWPNVNAVAQKLNKERGLEG
                     KIRFIDGEYLTAPIENSHYDIVLVSNFCRFESPKRNQKLFANAYSALKPGGYFIVNDF
                     VPNEERTEPAFALRFSVYTLTHTPEGECWTLSQYTEWMHAAGF"
     gene            complement(961..1764)
                     /locus_tag="VF089_12380"
     CDS             complement(961..1764)
                     /locus_tag="VF089_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174805.1"
                     /translation="MSELRSVKKDYSAKRLLPEEADAAIAELRKYLLELADRWLFKGR
                     FHTDMAAGVLPMEAIKVFWQNWYGFVAEINNFHGVAYQRHLPFFKRHPELQKHFANHV
                     ADEMVHPTPPGHIQIVLEQGRNFGLTNDDMIEYEMLAECRAFLEYYRGVLYEGTMAEW
                     WASFCVEEAVGHWARFFGGALRKNYNLPEEKIVYFRVHAQADLEEHQGVMAHAELDWT
                     VLKKLLEEGRAETRPGFNLAYCVRMGTAYFAWFFEGVYQHIKKEGLYKI"
     gene            complement(1821..2252)
                     /locus_tag="VF089_12385"
     CDS             complement(1821..2252)
                     /locus_tag="VF089_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6174806.1"
                     /translation="MLILDEIVVRLKEIVGARLVSTSAIDKINYQHTFAYGAYRALPD
                     VIVRIESDPQSNHALAKILSFANEHKIPIVAEGGFGMGISSPLKGGILLDMLGMDKIL
                     EVNPRMQYIIAEGGASVYAIHYALCQHGLMLPNFGTHAPRS"
     gene            complement(2369..2572)
                     /locus_tag="VF089_12390"
     CDS             complement(2369..2572)
                     /locus_tag="VF089_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6174807.1"
                     /translation="MAEQQKQLQLREKIVLITGASRGIGAALKKHLYPEDVAGTIAFL
                     ACDEASMLTGSHILITGGSHLQL"
     gene            complement(2584..>3247)
                     /locus_tag="VF089_12395"
     CDS             complement(2584..>3247)
                     /locus_tag="VF089_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174808.1"
                     /translation="KMVDMLEELEKKKLDMSWFMLSRAQNLIRDADLIPRLRKVGLYQ
                     VLIGIDGGTDEEIAEARKGPMKVGVQELKDLIKFLRQNDISTIATYLNGFWEDDEAKI
                     RQRAEAVDEIDPDIVMIQLLNPIPGSPIYKKAVKENLIETDNLSLYDLEHCVMPTKYL
                     SRQQLGELTGWAFQSFYSKPGRIDRIVNGYTSPYVKMKFLSFKNNAAKYEKGAAEDAV
                     AI"
BASE COUNT          733 a          880 c          832 g          802 t
ORIGIN      
        1 taaaccctgc cgcatgcatc cactcggtat actgggataa cgtccagcat tccccttcgg
       61 gagtgtgtgt cagggtgtaa accgaaaaac gcaaggcgaa ggccggttcg gttctctctt
      121 cattgggcac gaagtcgtta acaatgaagt acccgcccgg ttttaacgcg ctgtaagcat
      181 tggcgaacag cttttggttc cgctttggcg attcaaaccg gcaaaaatta ctgacgagaa
      241 cgatatcata gtggctattt tcaattggcg cggtgaggta ctcgccatcg atgaagcgaa
      301 ttttgccttc cagcccgcgc tctttgttga gcttctgcgc taccgcgttg acgttcggcc
      361 aatccagctg cgtcactttt gccgatggat cccgagagag aattgtcatg ccgaagatgc
      421 ttgaaccccc ggcgatatcc agcacgttga tggctttccg ctcggtgccg atcttaagaa
      481 gatcgcacag cgctctggcg gccggctcgg cgataacaat gatccccaaa acgagtttct
      541 cccagacctc aagcgcctcg ccggtgtcca agcggctgaa gggttttccc gttttaagcg
      601 attctttgag atgagccatt ccatcccaca gcgccaatgc aacatgccgg aaatcgccga
      661 cgtaagacgg cttatcgcgc accaaaaaag cctcgctgat cggcgttaac tgatagtggc
      721 cattttgctt ctcaagcaac gccagagcca gcaggctatc gagcactata cgtgttgccc
      781 gcggatcggt tccggcctgg ccagctatct cgtctgccgt atgaaaacct ttgccgatga
      841 gagtaaaaaa atcgtactca attgatgcgt cgagaatttg gctattggca aaatccccca
      901 gtatccgcat caagggacgc ggtgaaagat tggattgcct tacgggtgat tcaggcatga
      961 ttagattttg tacagccctt cctttttaat gtgctggtag acaccttcga aaaaccaggc
     1021 gaagtaagcg gtacccatgc gcacgcaata agccagattg aatccgggcc tggtctcggc
     1081 gcgtccctcc tcgagcagct ttttcaacac ggtccaatcg agctccgcat gcgccatgac
     1141 tccttggtgt tcctccaggt cagcttgagc gtgaacgcga aaatagacga ttttttcttc
     1201 cggtaggtta tagttcttgc gtaacgcacc gccaaaaaac ctcgcccagt gtccaacggc
     1261 ttcttcgacg cagaaggaag cccaccactc ggccatggtg ccctcgtaaa gcacgcctcg
     1321 atagtattca aggaaggcgc ggcactcggc gagcatctcg tactcgatca tgtcgtcgtt
     1381 agtcagaccg aagtttcttc cctgttcgag gacgatctgg atgtgacccg gcggcgtcgg
     1441 atggaccatt tcgtcggcga cgtgattggc gaaatgcttt tgcagttccg ggtggcgttt
     1501 gaagaacggc aaatggcgtt ggtatgccac tccatgaaaa ttattgatct cagcgacaaa
     1561 gccgtaccag ttttgccaaa agactttaat cgcctccatc ggcaagacgc cggcagccat
     1621 atcggtgtgg aaacggccct taaacagcca gcggtccgcc agctccaaca ggtattttct
     1681 cagttccgcg atggctgcat cggcttcttc aggcagcagt cgctttgccg aatagtcctt
     1741 tttcacactg cgcagttccg acatgtcgtt ctccgtatca gtaaagagcg ccggtcttca
     1801 actgttaatc attgcgccga tcacgaccgc ggggcgtgag tgccgaagtt ggggagcatt
     1861 aggccatgct ggcacaatgc atagtgaatc gcataaacgc tcgcgccgcc ttcggcaatg
     1921 atgtactgca ttctcgggtt cacttcgagg atcttgtcca tgcccagcat gtcgagcagg
     1981 atgccaccct taagcggtga actgattccc attccgaatc cgccttcggc aacgatgggg
     2041 attttatgtt cattggcaaa gctgaggatc ttggccagcg cgtgattgct ttgcggatcc
     2101 gattcaatcc ggacgatcac atcaggcaac gctcggtaag cgccataagc gaatgtgtgc
     2161 tggtaattga ttttgtcgat ggcgcttgtt gaaaccaagc gcgcgccgac gatctccttg
     2221 agcctgacaa cgatctcatc gaggatgagc atgtgattag gtcatcatgt aattggcaac
     2281 gacggggttg cggagagaat catcacggag gcttgagatg agaaccagta gctgttccgg
     2341 agcgaaccct tgcctgccca gtgaccgttt agagctggag gtggctgccc ccggtaatca
     2401 ggatatggct cccggtgagc atactggctt cgtcgcatgc caggaaggca atggttcccg
     2461 ccacatcttc cggatagagg tgctttttaa gcgccgcacc tattccccgg ctggcgccgg
     2521 taatgagaac aattttttcg cggagctgca gctgtttttg ttgctcggcc atggagctaa
     2581 aaactagatg gccacggcat cttcggccgc gcccttttcg tatttggccg cattgttctt
     2641 gaaggacaga aacttcatct tcacatatgg agaagtatag ccatttacaa tgcgatcaat
     2701 gcgaccgggt ttactgtaga acgattgaaa tgcccagccg gtcagctcgc cgagttgctg
     2761 acgcgacaga tacttggtcg gcatgacgca atgctctaaa tcgtacaagc tcagattatc
     2821 ggtctcgatc agattttctt tcacagcttt cttgtagatc ggtgatccgg gaatcgggtt
     2881 aagcagttgg atcatgacga tatccggatc aatttcatcc acagcctcgg cgcgctggcg
     2941 aatctttgcc tcatcgtcct cccaaaaacc attcaaatag gtggcgatcg tcgagatatc
     3001 gttctgacgc aagaacttaa taaggtcttt caattcctga accccaacct tcatcggccc
     3061 cttgcgggct tcggcgattt cctcgtcggt gccgccatct ataccgatca gcacctgata
     3121 gagaccgact tttctcaaac gcggaatcag gtcggcatca cgaatcaaat tctgcgctct
     3181 gctgagcata aaccagctca tgtccaactt tttcttttcc agctcttcga gcatatcgac
     3241 catcttt
//
LOCUS       DASUTG010000539         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_379894, whole genome shotgun sequence.
ACCESSION   DASUTG010000539 DASUTG010000000
VERSION     DASUTG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379894"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VF089_12400"
     CDS             complement(<1..815)
                     /locus_tag="VF089_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174809.1"
                     /translation="MRHCFSYIILLLLVFSVHVSGHAADKIRIGFPDLAAPFVPLAIG
                     DKRGFFQEEGIVAESIRMNPAVALQALVRGEIDYYTVLGPGVAAAIRGVPVKLVAAYV
                     PVSPTALIARPEIRSVAELKGKTIGINAYGGALEAMGRLIVKHFGIDPDKEVKFLATG
                     PLDSRFGAMKQGLTLATLGGPPIDFLGEKLGFVVLTRAHELFSFPVSGLIASEKKLKE
                     RPQEIGRVIKAGIKANRYIRQNRDGTLPIMAQWLKIDKEMATRTYDSSIKTFS"
     gene            874..1077
                     /locus_tag="VF089_12405"
     CDS             874..1077
                     /locus_tag="VF089_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174810.1"
                     /translation="MKPRIAQISLIPRHTVALNQNSWLWRGNTPSDLFDLFYKSAVRI
                     PMVLERQTTEACERIHQAITKGC"
     gene            complement(1285..4419)
                     /locus_tag="VF089_12410"
     CDS             complement(1285..4419)
                     /locus_tag="VF089_12410"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174811.1"
                     /translation="MPEEKEYTFGPFRLNATREQLWRENQEIRLAPKVFAVLRYLLEH
                     PGELITKNTFFESIWPGLYVTDTVLAVHIGKAREALGDDPQKPVYIETVHRRGYRFIA
                     ASRSHDPRALPEAALEDLASTKPISSKPATTIGSQPLIGRNAELILLREKLDAAANGR
                     GGLVLISGPPGIGKTRLARELRDWTLKSGGLWLEGRYDKATSQPYQAWVEIIRDYLQQ
                     KPGRSLESLQERYREQLTKLVPEIAAAPSESIRNDPETERLMLFEGVTQFFLDISRQT
                     TLVLFIDDLQWSASLELLHHLARNIGHQSILIVATYRDDELKNNLTLWRTILAMNRER
                     LFHSLPLEPLSKNEIENLISSRSDEAVASQLAALVYRKTAGNPFFVEEVLHLLQQRKI
                     LFVSNSGWHVRDAEAVETPESVKAVINERLERLGKEAEELLRLASTIGREFPLRLVRE
                     LKAEAEDALVETLDRCEGAGLIFSQQLRGEEMYSFTHDVIQESLYESIGPARRRRHHL
                     RVGHAIEKLYTPRLEEWFDALSHHFLKGNQLAKALEYSLVAGDKATAICSWGRASVHY
                     QTAIELLGELEAVPRHRAEVLEKLALATMWLGKGKESLVHWEQALSEYERLGDAKKAG
                     AVHLRLYQQYYTAVGSRDREKGYRHSVEALQLLEPEGDSIALAQAYTRLGLISAHRQN
                     VPLSAGIAPLEKGLALAQRLGDGATLAEAEMSLAHVLVYHLGDIHRGLELAHEAYEAA
                     KKSDDTVLLAETVLRVAEGYMMLMDADRAWGWAKEAVDISTRSGAPRHQILSSLLIGG
                     AYILGGDGPQALQSLETARQMAKRTGVEFNQIPRPPVRLALIMVPFFLGDWDQCEREL
                     LKWQDWPSAAVATLTAWMSGWLSLEKGDLAGAKAHLREAVTLCDARGEKTLALAPLAL
                     LSEVSSKAGELAEAAAYLRRAQEITSQPEDWCGLMGKVFLAEGILAAAQKRWQEANTA
                     FEKATEIHRRYRLPYYEARALFEWAEMHLARQRTGDRKRASELLEQSLNIFQNIQAKK
                     MVEKTQALKHRLRM"
BASE COUNT         1079 a         1346 c         1269 g         1089 t
ORIGIN      
        1 tcactgaatg tctttatact ggaatcatac gtccgagtcg ccatctcctt atcgatcttc
       61 aaccactggg ccataattgg cagcgttccg tcgcgattct gacgaatata gcggttggcc
      121 ttgatacccg ctttgatgac ccgcccgatc tcctgcggac gctctttgag cttcttttca
      181 ctagcaatca aaccgctcac cggaaaacta aatagctcat gggcgcgcgt caggacgaca
      241 aagccgagct tctcaccgag aaaatcgatg ggcggcccgc ctaaagtggc aagggtcaat
      301 ccttgcttca tcgcgccgaa gcgcgaatcg agaggcccgg tggcaagaaa cttaacttcc
      361 ttgtcgggat cgatgccaaa atgcttcacg atcagtcgcc ccatcgcttc aagagcaccg
      421 ccgtaagcat tgatgcctat cgttttaccc ttcagttcgg caacagacct tatttctggc
      481 cgggcgatta gcgcggtcgg agaaacgggc acgtaagcag ccaccagctt cactggaact
      541 ccacggatcg ccgcggctac cccagggcca agcaccgtat agtaatcgat ttcgccgcgg
      601 accaatgcct ggagcgcgac ggcaggattc atcctgattg actcagcaac aattccttct
      661 tcttgaaaaa agcctctctt atcgccaata gcgagaggca caaatggggc agcgagatct
      721 ggaaatccga tccttatttt atctgcggcg tgacccgaga cgtggacaga aaaaaccaac
      781 agcaagagaa tgatgtaaga aaaacaatgt cgcataatca gctcccttca cttatctatt
      841 cacttacgcg cctagatagg aggactgtca tcaatgaaac cacggattgc gcagatcagc
      901 cttataccgc ggcacacggt agcgctcaat caaaattctt ggttgtggcg cggcaacact
      961 ccgagcgatc tctttgacct cttctacaag agcgcggtgc ggattcctat ggtcctggag
     1021 cgacaaacaa ctgaggcgtg tgagcggatc caccaggcga ttactaaagg gtgctgaacg
     1081 ctttggccaa ggtgaacgga tcgtaatcgc tattcctgcg tcatggttac ggctctaagc
     1141 cataaacgaa ttgatttgga gttccatttt tctgcctcgt cagagagccg gcccgatggg
     1201 aacttttggt ggatgggaca gaacggcgca gtcgatcgag tagttacgta aaatgaaggc
     1261 gactcctatt gtagttcgaa ccttctacat gcgtagcctg tgcttgaggg cttgcgtttt
     1321 ctccaccatt tttttggctt ggatattctg gaagatattg agggattgct caaggagttc
     1381 actcgctcgt tttcgatcgc cggttcgctg ccgcgctaga tgcatttcgg cccattcaaa
     1441 cagcgcccgc gcctcgtaat agggcaggcg atagcggcgg tggatctcgg tcgctttctc
     1501 gaacgccgtg ttcgcttcct gccagcgctt ctgggcggcg gcgaggatac cctcggccag
     1561 gaacaccttc cccataagac cgcaccagtc ctcgggctga gatgtaatct cctgcgcacg
     1621 gcgcaaataa gccgctgctt cggccaattc accagcctta gaagagactt ccgagagtaa
     1681 agcaaggggg gctagcgcta aggttttctc gccccgcgcg tcacacagtg ttacagcttc
     1741 acggaggtgt gccttcgctc ctgcgagatc gcccttttct aaggaaagcc atcctgacat
     1801 ccaagcggta agcgtcgcca cggcggcgga aggccagtct tgccacttca agagctctct
     1861 ctcgcactga tcccaatcac caaggaagaa cggcaccatg atgagagcaa gacggaccgg
     1921 cgggcgcggt atctgattaa attcaacgcc agtccttttt gccatctgtc gtgccgtctc
     1981 gagactctgg agcgcttgtg gaccatcacc ccccaatatg tatgccccac caatcaataa
     2041 agaggagagg atctgatgcc gcggtgcacc ggagcgggtt gaaatgtcta cagcttcttt
     2101 cgcccacccc caggcgcggt ccgcatccat caacatcata tatccctccg caacgcgtag
     2161 cactgtctcg gctagcaaaa ccgtatcatc gctcttcttc gctgcttcgt aggcttcgtg
     2221 cgctaactcc aaaccacggt ggatgtcgcc gagatggtag accagcacat gggccaagga
     2281 catttccgct tcagccagcg tcgcaccgtc tccaagccgc tgcgccagag cgagcccttt
     2341 ctccagagga gcgattccag cggacaacgg cacgttctgc cgatgggcgg agatcaagcc
     2401 taatcgcgta tatgcctgtg ctaatgcgat gctgtctcct tccggttcta ggagctgcag
     2461 agcctcaaca gagtgtcggt aacccttctc ccggtcacgg gaaccaaccg cggtataata
     2521 ctgttgatat aaccgaaggt ggacagcacc ggcttttttg gcgtcgccga gtctctcgta
     2581 ctcggacagc gcttgttccc agtgcaccag agactccttg cccttgccaa gccacatggt
     2641 agccaacgct agcttctcca gcacctccgc tcgatgccgg ggcaccgctt caagctctcc
     2701 caaaagctca atggcagttt gatagtgcac actggcgcgt ccccaactac agatggcggt
     2761 cgctttgtcg cccgcaacga gcgaatactc gagggccttt gccagctgat tgcctttaag
     2821 aaagtgatgg ctcagagcgt cgaaccactc ctccagacgc ggagtgtaaa gcttctcgat
     2881 cgcgtgcccc actcgaagat ggtgccgccg ccggcgcgcc gggccgatgc tctcataaag
     2941 cgactcctgg attacgtcat gcgtgaaact gtacatttct tctccgcgga gctgctgtga
     3001 aaagatgaga ccggctccct cacatcgatc gagggtttca actaacgcgt cctctgcttc
     3061 agcctttaac tctcgtacga ggcgcaaagg gaactcccgg ccgatggtcg aggccaagcg
     3121 cagtaactct tcagcctcct ttcccagtcg ctcgagccgt tcgttgatca cggccttgac
     3181 cgactccggt gtttccaccg cctcggcgtc tctcacgtgc cagccggaat tcgagacgaa
     3241 aagaattttc cgttgctgaa gcaagtgcag tacttcctcg acaaaaaaag gattgccagc
     3301 ggttttccgg tacaccaacg cggcgagttg tgatgcaacc gcctcgtccg acctgctgga
     3361 aatgagattc tcgatctcgt ttttgctcag aggctccaaa ggcagagaat ggaagaggcg
     3421 ctcccgattc atggccagga tcgtgcgcca cagggtaaga ttgttcttca gttcatcgtc
     3481 gcgataggtt gcaacgatca aaatggattg gtgtccgata tttcgtgcca gatgatgcaa
     3541 cagttccagg gacgcggacc actgcagatc gtcgataaat agcaccagag tcgtctggcg
     3601 tgagatatca aggaaaaact gcgttacacc ttcgaatagc atcagccgct cagtttcggg
     3661 atcgttgcgg atcgattcgg aaggtgccgc tgcaatctcc ggcacgagct tcgttagctg
     3721 ttctcggtat ctctcttgga ggctttccaa tgacctgccg ggcttttgct gcagataatc
     3781 tcgtatgatt tcgacccaag cttgataggg ttgactggtt gctttgtcat accggccttc
     3841 gagccatagc ccgcctgatt ttagcgtcca atctcgaagc tctcgcgcta agcgcgtctt
     3901 gccgatcccc gggggaccgg aaatcaagac tagaccgcct ctgccattgg cggcagcgtc
     3961 aagtttttct ctcagaagga tgagctctgc attccgcccg atcagaggct gtgagccgat
     4021 cgttgttgcc ggcttgctgc tgatgggctt tgtcgatgct aaatcctcta aagcggcttc
     4081 cggcagagcc cgggggtcgt gtgaacgaga ggcggcgatg aagcgatagc ctcgccggtg
     4141 cacggtttcg atataaaccg gcttttgagg gtcatcaccc aaagcctcgc gagctttgcc
     4201 gatgtggacc gccagcaccg tgtcggtcac gtaaaggcca ggccagatag actcaaagaa
     4261 ggtatttttg gttatcagct caccggggtg ctccagcaga taccgcagca cagcgaagac
     4321 cttcggggcc aggcgtattt cctggttctc tcgccacagc tgctctcgag tggcgttcag
     4381 gcgaaaagga ccaaaggtat attccttttc ttcaggcatg tctgctctcg gagcgagctt
     4441 atatcgaacg ttaagaaaaa ataaagcggg cttatagaaa gtttaaagta agcaaaaagt
     4501 tcgtttaaga ctttactgtg cctgctgact agcttgccct caaaagaact cacaagttca
     4561 aggaggacaa aggcatgaag gctcgcatgg agagaatgtt cgctggaatt cgtggatgtc
     4621 tgtgcatgat acgcaagcgc cggatgcgag acgactttcg atcaagcggc tgcaataaga
     4681 acttgggatg cgaagagctt gagagattca tctactggtg atcgcgtcga ccggagggtt
     4741 tggcctgacg attccgccca acgtgctgac gagagcggac aag
//
LOCUS       DASUTG010000540         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_823855, whole genome shotgun sequence.
ACCESSION   DASUTG010000540 DASUTG010000000
VERSION     DASUTG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823855"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF089_12415"
     CDS             complement(<1..474)
                     /locus_tag="VF089_12415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174812.1"
                     /translation="MKAARHMLASVRALGTERMQLLGVLTGGHFVIHWFQQFFPVILP
                     SIKSGLGLTNVEVGALTSAQQMIMGLGQLPVGLLADSMVRQRAKMLGLSLAAMGAAYF
                     LLGSPVFLWALLGSGLIGLGTALWHPTAAASLSNRFPEHRATALSIHGTGATISDT"
     gene            791..994
                     /locus_tag="VF089_12420"
     CDS             791..994
                     /locus_tag="VF089_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX6174813.1"
                     /translation="MAKYGTKAKEKVKKAMRERKKGTLKSGRSGKKVTSRKQAIAIGL
                     SEARRAGGKVPRKKSSTRKAKKK"
     gene            complement(1031..2623)
                     /locus_tag="VF089_12425"
     CDS             complement(1031..2623)
                     /locus_tag="VF089_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEX6174814.1"
                     /translation="MRRRDFLKSALVLAGSPLSVSAVRPSAGSAQPPRPQRFNYDWLK
                     NHARGLACSDHQAPDRLLPNTLTTLDYDAYQAIRFGADKALWLREGLAFRIAFFHRGP
                     MFKEPVRIHEIIDGQASAIVYDPAMFDFRRARVNAGDLPRDLGFAGFRVHFHTDWRAD
                     VAAFLGASYFRAVGSDYRQYGISARGLAVDTALDKGEEFPRFTDFWLQRPIKDSKQLI
                     VYALLDSPSVTGAYRFDIRPGATLIMDVDAAIYPRKTIERIGIAPLTSMYQHGENDRR
                     MASDWRPEIHDSDGLQLLTGNGEWIWRPLMNPSGIRVNSYLDANPRGFGLLQRDRIFD
                     HYQDDSVFYERRPSLWVEPKNRLGQGWGAGAVQLVELPAPDETFDNIVAYWNPAAKPQ
                     AGDELLFSYRLYWGSTMPYAPALAQVVATRTGIGGVIGQPRKYFSYRFAVDFTGGEFA
                     RLTKGAQLEPVISASRGEIEIASARPQVEIGGYRAMFDLKPTDGSTEPIDLRLYLRHG
                     EQPLTETWLYQWTPPPAAERKL"
BASE COUNT          713 a          815 c          834 g          553 t
ORIGIN      
        1 ggtatcgctg atcgttgctc cggtgccgtg aatcgaaagc gccgtagcac gatgttccgg
       61 gaaacgattc gacaacgatg cagcggcagt cggatgccac agcgccgtgc ccaacccaat
      121 caatcccgag ccaagcagcg cccagagaaa aacgggagaa cccaggagaa aataggcagc
      181 gcccatggct gccagtgata agcccagcat cttggcgcgc tggcgcacca ttgaatcagc
      241 gagcaatccc acggggagct ggcctaaacc catgatcatc tgctgcgccg acgtcaacgc
      301 gccgacttcc acattggtca atccgagacc cgatttgatc gagggcagga tgaccggaaa
      361 aaactgttga aaccaatgga tcacaaagtg gccgccggta agaaccccga ggagctgcat
      421 gcgctcggtg ccgagcgcgc gcactgaggc gagcatatgt cgggctgctt tcataactaa
      481 ttgcaggctg ctgtttttag gtcatagacc acgttaacgt gagaaccacg cgcgcgaatt
      541 ctctacgatt agctcatgca gcaaccggtt ggaaggggac gggatcgatg ctcgttcggc
      601 agaatcaggc cactgcacga tgtgtcgaaa ccgacaacct ttgcccgacc tacttgtcag
      661 gaaaacaaaa cccaataagc tggagtgaaa tggatattaa attctttctg cgcctgcaat
      721 cgaatttcaa ttgaatggta tcggtcacgc gccgcaaagt taccggcact aactgacagg
      781 aggagcaaat atggcgaagt atggaacgaa ggcaaaggaa aaagtgaaaa aggcgatgcg
      841 cgagcgcaag aaaggcacac taaagagcgg acggtccgga aaaaaagtga ctagccgcaa
      901 acaggctatt gccatcggcc tttccgaggc acgtcgcgcc ggcggcaagg taccgcgcaa
      961 gaaatcatcg acccggaagg cgaaaaagaa atagattggt tccgctcgag cttcgcaggc
     1021 agcttgatca tcatagcttt cgctcagccg ccggaggcgg tgtccactga tacagccacg
     1081 tttcggtcaa cggttgttcg ccgtggcgca ggtacaaccg gagatcaatg ggctcggtgc
     1141 tgccgtcagt gggtttcaag tcgaacatgg cgcggtaacc gccgatctcc acttgcggcc
     1201 gcgccgatgc aatctcgatc tcaccgcgcg aagcgctgat caccggctcc agttgcgcac
     1261 ctttggtaag cctggcgaac tcgcctccgg tgaaatcgac agcgaagcga tacgagaaat
     1321 attttcgcgg ttgaccgatc accccgccga taccggtccg agtggcgaca acttgcgcaa
     1381 gcgcaggcgc atacggcatt gtcgaccccc agtagaggcg atagctgaac agcagctcgt
     1441 cgccggcctg gggttttgcg gcaggattcc aataagcaac gatgttgtcg aaggtttcgt
     1501 cgggcgctgg taactccacc agctgcacgg cgccggcgcc ccaaccctga cccagacggt
     1561 ttttcggttc cacccagaga ctcgggcggc gctcatagaa aactgaatcg tcctgatagt
     1621 ggtcgaagat gcgatcgcgc tgcaacagtc cgaagccccg cggattagcg tcaagataag
     1681 aattgacccg aatccccgag ggattcatca acggccgcca gatccattcg ccgttaccgg
     1741 tgagcagttg tagcccatcg gagtcgtgaa tttccggacg ccagtcgctg gccatgcgtc
     1801 gatcgttctc accgtgttga tacatgctgg ttagcggcgc gatgccaatc ctctcaattg
     1861 ttttgcgcgg ataaatggcg gcatcgacat ccatgatcaa ggttgccccg ggcctgatgt
     1921 caaagcggta ggcgcccgtg acgctgggag aatccaggag cgcgtagaca attaactgtt
     1981 ttgaatcttt tatgggtcgt tgcagccaga aatcggtgaa ccgaggaaac tcctcgccct
     2041 tatccagagc cgtgtcaacg gcaagcccgc gggcagaaat cccgtactgt cggtagtcgc
     2101 tgcccaccgc gcgaaagtag ctcgcgccga gaaaagctgc gacatcggcg cgccaatcgg
     2161 tatgaaaatg cacccggaaa ccggcaaacc cgagatctcg cggcaagtcc ccggcgttca
     2221 cccgagccct gcgaaaatcg aacatcgccg gatcgtaaac tatcgcgcta gcctggccat
     2281 cgatgatttc atgtatgcgc accggctctt taaacatggg gccacgatga aagaacgcta
     2341 tacgaaacgc gagaccctct ctgagccaca gcgctttatc agcgccaaac cgaatcgcct
     2401 ggtaggcatc gtagtcgaga gtcgtgaggg tattcggcag cagccggtcc ggtgcctggt
     2461 gatcactgca ggccagtccg cgcgcgtgat tcttaagcca gtcataattg aacctttgtg
     2521 gtcgcggcgg ttgcgcgctt ccagccgagg gccgcacagc cgataccgat aggggtgatc
     2581 ctgccaggac caaggcagat ttaagaaaat cgcgccgtct cattttcatt gttgtcgcgc
     2641 aaggcgaccg agaaggtaat ttcggcgaga ctaacaattc aagcccaagt tgcaagagga
     2701 gcggccaaaa tctcgccgcc gaaaccactt gcgtcggcgg gaaactacac cgctcagctt
     2761 gtggcatgtg gggcaaccgg ccgcggcaag accgctatta cttattacta ttgaagaagt
     2821 tgctcgtgaa gccaagaaga caggataatg gggttttctg gaaagtgctg ccagaggttg
     2881 ctggactaga agatcctatt cctgttccga gcgat
//
LOCUS       DASUTG010000541         9225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2010458, whole genome shotgun sequence.
ACCESSION   DASUTG010000541 DASUTG010000000
VERSION     DASUTG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010458"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF089_12430"
     CDS             complement(<1..516)
                     /locus_tag="VF089_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX6174815.1"
                     /translation="MHFLRLLSIPALTAAFVAVPVLWAWPEGLTFWRTLAIVLGWAGC
                     GLLLASLLLILRETALAHSLGGLERMYRWHHFVGLGAYLLLLMHPLAFAVDAWRESPH
                     LAWQVLSPASQGWPVWLGWISLLFIMTGLAATFSAKVSYATWRWLHGLLSVGILFGML
                     HLVLLGISEAVI"
     gene            complement(547..849)
                     /locus_tag="VF089_12435"
     CDS             complement(547..849)
                     /locus_tag="VF089_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174816.1"
                     /translation="MRIKFIRLVILSMFLLAGPAHPRGPWRASETNTRGWQFMTPEER
                     IEHQAKVRGFKTYDECRAYQVEHHQLMEERARQRRMALPSGGLDFCAHLKPGTESR"
     gene            complement(877..1860)
                     /locus_tag="VF089_12440"
     CDS             complement(877..1860)
                     /locus_tag="VF089_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX6174817.1"
                     /translation="MPVKYKNTEMSQGSTIDQMHEPANGRKLMLATVLTLGFAGVEAA
                     TGWVSGSLALLSDAGHMVTDAAALMIAALAGAIARRGPSPKHSYGFGRAQLVAVLVNG
                     LFMLAVVTAIIIQAVSRFANPIAVQGEAVTVVALLGLLLNILVARMLSHGGRDLNMRA
                     ALLHVMGDLLGSLAALLSGIIVWATGWFAVDPALSLFIALLIAYSSLKLVREAFHGLM
                     EGVPLHLSLEEIGKSMANVEGVTSVHDLHVWSLTAERVALSAHVVVEPLDDWPATLER
                     LRLLLAQRYGIEHVTLQPEIGEQRPAAVSAPRVISLTENFTAQRPAQRRDR"
     gene            complement(1885..2724)
                     /locus_tag="VF089_12445"
     CDS             complement(1885..2724)
                     /locus_tag="VF089_12445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HEX6174818.1"
                     /translation="MSGRGEIEQRFQRLREISGIMTAMKSLSLVETRKLARFIGHQQR
                     MLANIEATAADFLHFFSAEIAAKTEGTILLLVGSERGFCGNFDERILTALRELPLSKS
                     APRLLVVGRRLGAKLEAHPDVIAFLDGPTVTEDVPAVLDRLMDAVYVARQRLAGDGVA
                     LSALAHDAEGEPRLKCLLPLSPPGPPRFGHPPRLQLAPAEFLHQLMDHYLLAAMYGLL
                     YGSLAAENRRRLEHMEHALRRLDETIARLAIRRNALRQEEIVEEIEVILSSEQAFAER
                     RKS"
     gene            complement(2721..4190)
                     /locus_tag="VF089_12450"
     CDS             complement(2721..4190)
                     /locus_tag="VF089_12450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6174819.1"
                     /translation="MASEAGVLERLDNRVARYRFGLRLGEQGRVASVGDGIAWVYGLP
                     SAAAEEILRFEDGSRGLVFDLQAERLGVILLDPAAKVTSGSLVSHTGERLEIGVGDGL
                     LGRVIDPLGKPLDGLAPPKPDGVCALETPSPPIVVRDSVIHPLYTGIKVIDILIPIGR
                     GQRQLIVGDSGTGKTSLALDSIISQAGQQVNCVYVSIGQKRSETASVVDILRRRGALA
                     FTTVVVAEATATPGMKYLGPFAGCAVAEGWMRRGKDTLVVYDDLTTHARAYRELSLLM
                     HRPPGREAYPGDIFYLHARLLERSTRLAANHGGGSLTALPIVETEEGQIAAYIPTNLI
                     SITDGQIYLDGRLFAAGILPAVDVPLSVSRIGGKAQHPRIKEEAGRMKLDYLQFLELE
                     IFTRFGTKLETGTATKIKRGRLLREILKQERLSPLPAEFQLAWMIAYNEGLLDSLEPK
                     ELTVRLRVLQEQIKGQLLPLDAGRDEWLEKLRSCLGTTT"
     gene            complement(4180..4929)
                     /locus_tag="VF089_12455"
     CDS             complement(4180..4929)
                     /locus_tag="VF089_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174820.1"
                     /translation="MDFDWTTFALEAVNFLILVWILKHFFYRPVLAVIETRRAESEKN
                     LAQADTLRSEAQSLKDEYANRLDAWERERVAAKARLDEEISAARAARLAALETEIAQE
                     RKRREALESRSRSDRQHILERQAVTIAARFAARLLDRLAGPELEAKLVDLALSELVTE
                     PPDKLDALRAALQEPGLRVKVVTAYPLDETRRSAFVQAFSQLAGRPVAPSFAEDSIVR
                     AGTCVMAGSWVLMANLRDELSFFSEMFDHGE"
     gene            complement(4937..5203)
                     /gene="atpE"
                     /locus_tag="VF089_12460"
     CDS             complement(4937..5203)
                     /gene="atpE"
                     /locus_tag="VF089_12460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014234581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX6174821.1"
                     /translation="MNDMTWFTLLSTVVGLAAITLGVIFPALAMGRAISQALDSLARQ
                     PEAEKSITRTLFIGLAMIESLAIYVLVVVLIVLFRNPLLQYLLK"
     gene            complement(5203..5889)
                     /locus_tag="VF089_12465"
     CDS             complement(5203..5889)
                     /locus_tag="VF089_12465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6174822.1"
                     /translation="MGKPELLEALAFDVGPFHVGTTVLTTWLLMALLAALAYFGTRRL
                     RVHAPTRLQTGLEATALALRGAIEAVAPEHATRLLPFIGTLWIFIVAANLIGLVPGLR
                     SPTSDLSLTVALAVLVFLSVHWFGVSIDGITGYLRHYLAPNPILLPFHVLGEITRTLA
                     LAVRLFGNMMSLEMAALLVLLVAGLFAPVPLLMLHIVEALVQAYIFGMLALVYVAGTL
                     QSRELEKRSN"
     gene            complement(5890..6162)
                     /locus_tag="VF089_12470"
     CDS             complement(5890..6162)
                     /locus_tag="VF089_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6174823.1"
                     /translation="MQDSERLRKNIEKQARRTEQAAKDRSTILAQTVFLGTLALLFVL
                     PVVGGAYLGSWLDGLREGYSYRWTMSLLVTGVFIGAMNVYLYIQRH"
     gene            complement(6168..6563)
                     /locus_tag="VF089_12475"
     CDS             complement(6168..6563)
                     /locus_tag="VF089_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6174824.1"
                     /translation="MNTFTLHLYAADSYERIDEVASFVGEDESGSFGLLPRHDRFMTV
                     LTFGLARLRRGDGATEYLGLPGGVLYFVDNALYLSTRRYLRDTDARRIAEALAGELLR
                     EEQALVETRRKLHLLEAEMLKRLVELGRE"
     gene            complement(6560..7978)
                     /gene="atpD"
                     /locus_tag="VF089_12480"
     CDS             complement(6560..7978)
                     /gene="atpD"
                     /locus_tag="VF089_12480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6174825.1"
                     /translation="MSLKIISHPQDIVAVEPARVPIGRVAEVHGPVVDIACETLPPLH
                     RALQLSIDGGSAVLEVLHHLDSHHVRAIALHRTAGLQRGTPVFDSGGPLRVPVSGKCL
                     GRLLDVFGAPLDGGPPLAPGEFRDLLSPPPSLGETMPSSGILETGIKVIDLLCPFIRG
                     GKTGLFGGAGVGKTVLIMEFMQAVASLHGGVSVFAGVGERIREGHELWHEMGENGALA
                     HALLIFGQMDESPGVRFRVGLTALAYAEFLRDTLNKEVLFLMDNAFRFVQAGSEVSGL
                     LGRMPATVGYQPTLPSEVAELEDRIVSCRRGDITSVQAVYVPADDMTDPAVGAILAHL
                     DTRVILSRAQAGKGIYPAVDPLVSGSKLVDRYFLGERHYTVGQDVREHLARYRELEDI
                     ITMLGIEELSEEDRRVVRRARKLQRYLTQPFHSLAAHMGVPGATVPLAQTLADCEAFL
                     RGDYDNLSEERCYMRGAMEVAP"
     gene            complement(7969..>9225)
                     /locus_tag="VF089_12485"
     CDS             complement(7969..>9225)
                     /locus_tag="VF089_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174826.1"
                     /translation="LDPDRLRRELEAEPSLAGLDENIARTRPHLFSATAAFMSREHFS
                     GIQALIDAVESVVSLPGYQESALARAPRVAQLDPGPLGVFLGFDFHLTSGGAQLIEIN
                     TNAGGALLNTVLARSQQACCREMFTEFEPTADLENLEKIFFTMFYEEWRRQRGAALLG
                     PVAIVDDNPSGQYLYPEMRLFERMFARFGVEAVIADACELIWRDGRLWHGERTVELVY
                     NRLTDFYLEDPAHLALRAAYEAGAVVVTPHPRAHAMYADKRNLVTLSDDRTLAALGVP
                     AEARAILVAGVPRTESVTPGRAEALWGERRKLFFKPAAGYGSKAAYRGDKLTRRVWQE
                     VLHGNYVAQALVTPSERRLEVDSAETDLKLDVRAYVYGGAIQLIAARLYEGQTTNFRT
                     PGGGFAPVFVVNAESSDVCDPGQRCL"
BASE COUNT         1857 a         2958 c         2793 g         1617 t
ORIGIN      
        1 gatcacagcc tcactgatcc ccagcagaac aagatgcaac atcccgaaca gaatccctac
       61 gctgagcaag ccatgaagcc agcgccaggt tgcataactg actttggccg agaaggttgc
      121 cgcaagtccg gtcatgatga atagcaagga aatccaaccc agccaaacgg gccagccctg
      181 gcttgccgga gaaagcactt gccaggccag gtgcggtgat tcccgccatg cgtcaacggc
      241 gaacgccagc ggatgcatca gaagcaacaa gtaagcgccc aagccgacga aatgatgcca
      301 gcggtacatc cgttccaagc cgccgagcga gtgggccagc gcggtttctc tgagaatcag
      361 tagcaagctg gccaggagca ggccacaacc ggcccaaccc aagacgatgg ccagcgtccg
      421 ccagaacgtg agtccctccg gccacgccca gagaacggga accgccacga atgctgcggt
      481 gagcgccggg atcgacagga gacgaaggaa gtgcattgcg gtacttttct tttgataacg
      541 tcgccgctat cgggattcag ttccgggttt caagtgcgcg caaaaatcca gcccaccgga
      601 aggcagggcc atgcgacgct gcctggcgcg ctcctccatg agctggtggt gctccacctg
      661 atatgctctg cattcgtcgt aggtcttgaa gccacgaacc ttggcctggt gttcgatccg
      721 ttcttcaggc gtcatgaact gccagccacg ggtattcgtt tcgctagcac gccaggggcc
      781 ccttgggtgg gccgggcccg ccagcagaaa catggacaga atgaccaatc tgatgaattt
      841 tattctcatc ccgtgtgttc cttggcggtg ttctcgtcaa cgatcacgtc gttgcgcggg
      901 gcgttgcgct gtgaagtttt cagtcaaaga tatcactcgc ggcgccgata cagccgccgg
      961 cctctgctcg ccgatctccg gctgcagggt cacatgttcg atgccatagc gttgtgccag
     1021 cagcaacctc aggcgctcca gagttgccgg ccagtcatcc agcggttcca ccacgacatg
     1081 cgccgagagc gcgacgcgtt cggcggtgag agaccagacg tgtagatcgt gaaccgatgt
     1141 cactccctct acattggcca tgctctttcc gatttcttcc agcgataaat gcagtggcac
     1201 accttccatc aacccgtgga acgcctcgcg cacgagtttc aggctggaat aggcgatgag
     1261 cagagcaatg aaaagggaga gcgccgggtc gacggcgaac caaccggtcg cccatacgat
     1321 gatgcccgag agcaacgccg ccagcgagcc cagcagatcg cccattacgt gtaaaagggc
     1381 ggcgcgcatg ttgagatcgc gccctccgtg cgagagcatc cgagctacca agatgttgag
     1441 cagcaggccc agtaacgcca ccactgtgac cgcctcaccc tggacagcga ttggatttgc
     1501 gaaccgtgac acagcctgga tgatgatggc cgtcactacc gcgagcatga atagcccatt
     1561 taccagcacc gcgactaatt gcgcgcgtcc aaatccgtaa gaatgcttcg gcgaaggccc
     1621 gcgccgcgcg atcgcgccgg cgagcgccgc gatcatcaat gctgccgcgt cggtgaccat
     1681 gtgtcctgcg tcgctcagca gagcgaggga gcctgacacc caacccgtcg ccgcctcgac
     1741 ccccgcaaaa ccaagcgtca acactgtggc gagcataagc ttgcgaccat tggcgggctc
     1801 atgcatctga tcgatggtag aaccttgcga catctcggta tttttatatt taaccggcac
     1861 tggagcggct cacgtggttt ccgatcagct cttgcgcctt tcagcgaacg cctgctcgct
     1921 ggatagaatg acctcgatct cctcaacgat ctcttcctgg cgcagggcgt tgcgcctgat
     1981 ggcgaggcgc gcgatggtct catcgaggcg acgcaaagca tgctccatat gctccaagcg
     2041 gcggcggttc tccgccgcca gcgaaccgta cagcagaccg tacatggcgg cgaggagata
     2101 atgatccatg agctggtgga gaaactctgc aggcgccagt tgaaggcgcg gtggatggcc
     2161 gaagcgcggc ggccccggcg gcgacaaagg cagcaggcac ttcagccgcg gctcgccttc
     2221 tgcatcgtgg gccagcgcgc tgagcgccac cccatcgccc gcgagtcgtt ggcgtgccac
     2281 gtacacggca tccatcaagc ggtcgagcac ggcgggcaca tcctccgtca cggtagggcc
     2341 atcgaggaaa gcaataacgt cgggatgcgc ctcgagcttt gcccccaggc ggcgacccac
     2401 caccagcagc cggggcgcgg atttcgacag cggcagttcc cgtagcgcgg taaggatgcg
     2461 ctcgtcgaaa ttgccgcaga agccgcgctc cgagccgacc agcagcagaa tcgtaccctc
     2521 tgttttcgcc gcgatctcgg ccgaaaagaa atgcaggaaa tcagcggccg tggcctcgat
     2581 gttcgcgagc atacgctgct ggtgaccgat gaagcgcgcg agcttgcgcg tctctacgag
     2641 agagaggctt ttcatcgcgg tcataatgcc gctgatttcc cgcaggcgtt ggaagcgctg
     2701 ctcgatttcc ccgcgaccgc tcatgtcgtt gtgccgaggc agctgcgaag cttttccagc
     2761 cactcgtctc gaccggcatc gaggggcaat agttgaccct taatctgctc ctgcagcacg
     2821 cgcagccgta cagtcagctc cttcggctcg agagaatcga gcaggccctc gttataggcg
     2881 atcatccacg cgagttggaa ctccgcgggt aatgggctca gtcgttcctg cttgagaatc
     2941 tcgcgcagta gccgcccgcg cttgatcttg gtcgctgtac cagtctccag cttggtgccg
     3001 aagcgggtga agatttcgag ctcgagaaac tgtagatagt cgagcttcat ccgtccggcc
     3061 tcctctttga tgcgcgggtg ctgcgccttg ccgccgatgc gcgacaccga gagcggcaca
     3121 tccaccgcgg gcagaatgcc ggcggcaaac aaccgcccgt ccagataaat ttgcccatcg
     3181 gtgatcgaga tgagattggt tgggatatag gcggcgatct ggccttcctc ggtttcaaca
     3241 atcggcaaag cggtaaggct accgccgccg tgattcgctg ctaagcgtgt ggagcgttcg
     3301 agcaggcgcg cgtgcaggta gaaaatgtcg ccagggtaag cctcgcgtcc cggtgggcgg
     3361 tgcatcaaca atgacaactc ccggtaggct cgggcatgcg tggtgaggtc atcgtaaacc
     3421 accaaggtgt ctttgccgcg gcgcatccag ccctcggcga cggcgcagcc cgcgaagggc
     3481 cccagatact tcatgcctgg cgtagcggtg gcctcggcca ccaccaccgt tgtaaaggcg
     3541 agcgcgccgc ggcggcgcag gatatcgact accgacgccg tctcggaacg cttctgtccg
     3601 atcgagacat agacgcagtt cacctgctgc ccggcctggc tgatgatgct gtcgagcgcg
     3661 agcgaggtct taccagtgcc gctatcaccg acgatgagtt ggcgctgccc gcggccgata
     3721 gggatcagga tgtcgataac cttgatgccg gtgtaaagcg ggtgaatcac ggagtcccgc
     3781 acgacgatcg gcggcgaagg cgtctccagc gcacagacgc cgtccggttt gggcggtgcc
     3841 aagccatcaa gtggcttgcc aagcggatcg atgacccgcc cgagcaggcc atcgccgaca
     3901 ccaatctcaa gccgctcgcc ggtatgcgaa acgagcgagc cggaggtcac cttcgccgcc
     3961 gggtccagga ggataacccc caggcgctct gcttggagat cgaacaccag tccccggctg
     4021 ccgtcctcga agcgcaagat ttcttccgcg gcggcggaag gcaggccgta gacccaggcg
     4081 ataccgtctc ccactgacgc gacccgcccc tgctcgccca gccgcaggcc gaagcggtag
     4141 cgcgccaccc ggttgtccaa gcgctccaag actccggctt cactcgccat gatcaaacat
     4201 ctccgagaag aaactcaatt cgtcgcgcag gttggccatt agtacccacg agcccgccat
     4261 cacgcatgtg cccgctcgca caatcgaatc ttcggcgaaa gaaggggcga cgggacggcc
     4321 ggcgagttgg ctgaaggcct gcacaaaggc gcttcgccgc gtctcgtcga gcgggtaggc
     4381 ggtgaccacc ttgacgcgca agcccggttc ctgtagcgcc gcgcgcaggg catctaactt
     4441 gtccggtggt tcggtgacga gttcgctcaa ggccaggtcc accaacttcg cttccagctc
     4501 ggggccagcc agtcggtcca gcagtcgcgc ggcaaagcgg gcggcaatcg tgacggcctg
     4561 ccgctccagg atgtgctgcc gatcgctgcg cgatctgctt tcaagagcct cccgccgctt
     4621 gcgctcctgc gcgatttccg tctccagagc ggccaggcgc gccgcgcgcg cggccgagat
     4681 ttcctcgtcc agccgcgctt ttgccgccac tcgttctcgt tcccaggcat ccagccggtt
     4741 tgcgtactcg tctttcaacg attgtgcttc gctgcgcaag gtatcggcct gagcgagatt
     4801 tttctcgctc tccgcccgac gggtttcgat caccgccaac accgggcgat agaaaaaatg
     4861 cttcagtatc cataccagga tcaggaagtt caccgcctcc agcgcaaagg ttgtccagtc
     4921 gaagtccatc gtgcgcctat ttcaaaaggt actgcagcag tggattgcgg aacagcacga
     4981 tcagcaccac caccagcacg tagattgcga gcgactcgat catcgccagg ccaatgaaca
     5041 gcgtgcgcgt gatggatttt tctgcctcgg gttggcgcgc cagcgagtcc agcgcctgac
     5101 tgatggcgcg tcccatggca agtgcgggga aaatcacgcc gagggtgatc gcggcaagcc
     5161 cgacgacggt ggacagtagc gtaaaccagg tcatgtcatt catcagttgc tcctcttctc
     5221 caactcgcgg gattgcaagg taccggcgac gtagacaagc gccagcatgc cgaagatgta
     5281 ggcctgtacc agcgcctcga caatgtgcag catgagcagc ggcaccggcg cgaaaaggcc
     5341 cgccacgagc agcaccagca gcgcggccat ctccaggctc atcatgttac cgaacaggcg
     5401 aacggcgagg gcgagcgttc gggtgatctc accgagcacg tgaaagggca gcagaatcgg
     5461 gttcggcgcg agatagtggc gcaggtagcc agtgatgccg tcgatactga cgccgaacca
     5521 gtgcaccgaa agaaacacta ggacggccag cgctacggtg agggagagat cgctcgtggg
     5581 ggagcgcagt cccggcacca gcccgatcag gttggccgct acgatgaaga tccacagagt
     5641 gccgatgaac ggcagcagtc gggtggcatg ctccggcgca acagcctcaa tggcgccgcg
     5701 cagcgcaagc gccgtcgctt ctaatcctgt ctgcaggcgc gttggcgcgt ggaccctcaa
     5761 gcggcgggtg ccgaagtagg caagggcggc cagcaatgcc atcaggagcc aggtggtgag
     5821 caccgtggtg ccgacatgaa atggccccac atcaaaggcc agcgcctcga gcagttccgg
     5881 cttacccatt caatgcctct ggatgtagag ataaacgttc atggcgccga taaaaactcc
     5941 cgtcaccagc aggctcatcg tccagcggta ggaatagccc tcccgcaggc catccagcca
     6001 actcccgaga taggcgccgc ctaccacggg cagcacgaaa agcagcgcca acgtgccgag
     6061 gaataccgtc tgcgctagga tagtggaacg atccttcgcc gcctgctccg tgcgccgcgc
     6121 ttgtttctca atatttttac gcagccgctc gctatcctgc atggacgcta ctcccgtccc
     6181 agttcgacga ggcgcttgag catctcggcc tcgagtagat gcagcttgcg ccgcgtctcc
     6241 accagggcct gttcttctcg cagcagttcc cccgccagag cctcagcgat gcgccgcgca
     6301 tcggtgtcgc gcaggtagcg ccgggtgctc aaatagagcg cgttgtcgac gaaatagagc
     6361 acgccgcccg gcaggcccag gtattccgtg gcgccgtcgc cgcggcgcag ccgcgcgagg
     6421 ccgaaggtca gaacggtcat gaagcggtca tgccggggta ggagaccaaa gctgccgctc
     6481 tcgtcctcgc cgacaaagct cgctacctcg tcgatacgct cgtaactgtc ggccgcataa
     6541 agatgcagcg tgaaggtatt catggcgcca cttccatggc gccgcgcata tagcagcgct
     6601 cctctgatag attgtcgtaa tcgccgcgca ggaaggcctc gcaatccgcc agcgtctgcg
     6661 ccagcggcac cgttgcgcct ggcacaccca tgtgggcggc cagcgaatgg aacggttgcg
     6721 tcaggtaacg ctgcagcttg cgcgcgcgtc gcacaacgcg gcgatcttcc tcagacagct
     6781 cctcgatgcc gagcatggtg atgatgtctt ccagctcacg atagcgggcg agatgctcgc
     6841 gcacgtcttg tcccacagta tagtgccgct cgcccaaaaa ataccggtcc acaagcttgc
     6901 tccccgagac gagcggatcc accgccgggt agatgccctt gcccgcctgg gcccgcgaca
     6961 ggatcacacg tgtgtccagg tgagcaagga tggctcccac cgcggggtcg gtcatgtcgt
     7021 cggcgggcac gtagacggct tgcacagagg tgatatcgcc gcgccggcat gatacgattc
     7081 ggtcctccag ctccgcaacc tcggagggca aggtgggctg atagccgacg gttgcaggca
     7141 tgcggcccag cagcccggac acttcggacc cggcctgcac gaagcgaaag gcattatcca
     7201 ttagaaacag cacttccttg ttgagcgtgt cgcgcagaaa ctcggcgtag gcgagggcgg
     7261 tcaagcctac gcgaaaacgc acgcccggtg attcatccat ttgtccgaaa atcagcagcg
     7321 cgtgggccag tgcgccgttt tcccccatct catgccacag ctcgtgccct tcgcggatgc
     7381 gctccccgac ccccgcgaac accgacactc cgccgtgcag ggaagcgacc gcctgcatga
     7441 actccatgat aagcaccgtc ttgcccaccc ccgcgccgcc gaagagtccg gtcttgccgc
     7501 cacggatgaa cgggcaaagc agatcgatca ctttgatccc ggtctcaagg atgccgcttg
     7561 acggcatcgt ctcgcccagc gacggcggtg gggaaagcag gtcgcgaaac tcgccgggag
     7621 cgagcggtgg cccgccgtcc agaggcgcgc cgaacacgtc gagcaatcgg ccgaggcatt
     7681 tgccggacac cggcacccgc aatgggccgc cgctgtcgaa gaccggcgtg ccgcgctgga
     7741 gccccgccgt gcgatgcaga gcgatggcgc gcacgtggtg tgaatcgagg tggtggagca
     7801 cttcgagcac cgcgctcccg ccgtcgatgg acaactgcag cgctcggtgc agcggcggca
     7861 gtgtctcgca ggcgatgtcc accaccgggc cgtgcacctc cgcgacgcgg ccgataggga
     7921 ctctagctgg ttctacagca acgatatcct gcggatgaga tatgatcttc agagacatct
     7981 ctgacccgga tcacagacat cactgctctc tgcgttaaca acaaataccg gtgcaaaacc
     8041 tccgccggga gttcggaaat ttgttgtctg tccctcgtaa agacgggctg caattaattg
     8101 gatcgcgcca ccatagacgt aggcgcgcac atcgagcttc aggtcggtct cagcgctatc
     8161 gacttccagg cgacgctcgc tcggggtcac gagcgcctga gccacataat taccgtggag
     8221 tacttcctgc cacaccctgc gcgtgagttt gtcgccgcgg taggcagcct tgctgccgta
     8281 gcctgccgcg ggcttgaaaa aaagcttccg gcgctccccc cataatgctt ccgcccgccc
     8341 cggcgtcacg gattcggtgc ggggaacacc cgccacaaga atggcgcgag cttctgcggg
     8401 aacacccagc gctgcaagag tgcggtcatc gctcagcgta acaagattgc gcttatcggc
     8461 gtacatagca tgggcgcgag gatgaggcgt caccaccacc gcaccggcct cgtaggctgc
     8521 gcgtagcgca aggtgagcag ggtcttccag atagaaatcc gtcagtcggt tgtaaaccaa
     8581 ctccaccgtt cgctccccat gccacagccg cccatcccgc cagatcagct cacaagcatc
     8641 cgcgatcact gcctccactc cgaagcgcgc gaacatgcgc tcgaagagcc gcatctccgg
     8701 atagagatac tggccgctgg ggttatcatc cacgatagcc actgggccca gcagagccgc
     8761 gccgcgctgg cggcgccact cttcgtagaa catcgtgaag aagatttttt ccaggttttc
     8821 caggtcggct gtcggttcga actcagtgaa catttctcga cagcacgcct gctgcgagcg
     8881 ggccaacacc gtattgagca gcgccccacc cgcgttggta ttgatctcaa taagctgcgc
     8941 gcctccggat gtcaggtgga aatcgaaacc caggaacacg cccagagggc ctgggtcaag
     9001 ctgggcgacg cggggcgccc tggccagcgc cgattcctga tagccgggta ggctcacgac
     9061 gctttccact gcgtctatca gcgcctgtat gccactgaag tgctcccggg acatgaaagc
     9121 ggccgtggca gagaacagat gcggccgcgt gcgcgcaatg ttctcgtcca ggccggcgag
     9181 ggagggttcc gcttccagct ctcggcgcag ccgatcgggg tccag
//
LOCUS       DASUTG010000542         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1418491, whole genome shotgun sequence.
ACCESSION   DASUTG010000542 DASUTG010000000
VERSION     DASUTG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418491"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1120)
                     /locus_tag="VF089_12490"
     CDS             complement(167..1120)
                     /locus_tag="VF089_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6174827.1"
                     /translation="MAVIPLKPVDRVEVLSVMDNSIDVLMGNTPVARRLKRTRDSHTR
                     PQLRAEHGVSMLVSTYEGGNRDRFLFDTGVTLDGVLHNMDVLEIKGNELHAVVLSHGH
                     TDHTRGLMGFIKRYGRPKIPIVLHPDAYLKRKNVFPDGHESEHIPPSKRDLEAEDVQI
                     VEDRGPSMLIGGRALVTGQIPRSTGYEKGSPNQVAWIDGKWQPDPWIHDDQAIVLNVK
                     EKGLVVLTGCGHAGVINTLRCARELTGVDQVHAVIGGFHLTGPVFEPIIKPTIEALKD
                     FAPGIIVPQHCTGWRATHMIARDFPDAFVPNSVGTTLVIGG"
     gene            2161..3573
                     /gene="pyk"
                     /locus_tag="VF089_12495"
     CDS             2161..3573
                     /gene="pyk"
                     /locus_tag="VF089_12495"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6174828.1"
                     /translation="MRRTKIICTIGPATESAEMLRKLVEAGTDVFRLNFSHGTVSGHE
                     SIIRRIRDVAEETGKPVAILQDLPGPKIRLGVFKDGSVELHAGDRFRLTTDEVPGDHE
                     CASVSYSKLPDEVRPGQSILLADGTVELEVESAAPRKIVCRVVLGGVLTNHKGVNVVG
                     GSSSLPAFTERDQKLLLSGLEASVDLVALSFVRSPVDIQSARTFLAAHQANLPLMAKI
                     EKLESLDIIDEILAVADSIMVARGDLGLEVPIAQVPHVQKELISKAIQAAKPVVTATQ
                     MLRSMVENPRPTRAEIADIANAVLDGSDALMLSEESAVGAYPVEAVKVLAETAEVAET
                     RLFARRPFLCLSPTDRVSAAEAIAHAASLVAHQAKATAIICCTRTGQTARLVAKYRPV
                     QPIIAMTHEPQTVRPLMIVWGVQPFLLDECRSIDATIKAAIDLASKKKIVKPGKVVIV
                     AAAPNSPRGETDFLRVANLK"
     gene            complement(3727..>4032)
                     /locus_tag="VF089_12500"
     CDS             complement(3727..>4032)
                     /locus_tag="VF089_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174829.1"
                     /translation="QICCRNIAVGKGHASRALALEQMAILLRPCLYVIKVGTNAARLV
                     LSDPEVRKAVSLSRHSEAIRFLPLAVTYAIKNILSLSWRGKEEVKPEDWVLTAPKPA"
BASE COUNT          915 a         1098 c         1076 g          943 t
ORIGIN      
        1 tctttcgcca aatgaaaccc attctgcggc aaagagagcg ccgggacagg agactcggtg
       61 gtacgatgcc acatcttaat tcgctgtttg agtcttgctg tgtgaatggg taaaggcccc
      121 aaagatgtga ctgtatgtaa aaccttgagc ttatgcgctc cgcgccttag ccaccgatca
      181 ccaaggtggt gcccacacta ttcggcacga aagcatcggg gaaatctcgc gcgatcatgt
      241 gggttgcgcg ccagccagtg cagtgctgcg gaacaatgat gccgggagcg aaatctttta
      301 acgcctcaat cgtcggctta atgatcggct cgaacaccgg gccggtcaga tgaaatccgc
      361 caatcacggc atggacttga tctacaccgg tcaattcgcg cgcgcatctg agcgtattga
      421 tgacgccggc gtggccgcag ccggttaaaa ccaccagccc cttctctttc acattcagga
      481 caatcgcctg atcgtcatgg atccaggggt cgggctgcca ctttccatcg atccaggcca
      541 cctggttcgg gctgcctttt tcataacctg tgctgcgagg gatctgccct gtgaccagcg
      601 cccgaccgcc gatcagcatt gacggcccgc ggtcttcgac gatctgcaca tcttcagctt
      661 cgaggtcgcg tttgctcggc gggatatgtt cggactcatg gccatcggga aaaacgttct
      721 ttcgtttcag ataggcgtcg ggatgaagca cgatcgggat tttaggtcga ccatagcgtt
      781 tgataaagcc catcaggccg cgtgtatgat cggtatggcc gtgactcaaa actacggcat
      841 gcagctcgtt gcccttgatt tccaggacat ccatgttgtg caacacgccg tcgagcgtca
      901 cgccggtgtc gaagagaaag cgatctctgt tgccgccttc gtatgtcgat accagcattg
      961 aaacgccgtg ctcggcacga agttgcggcc gggtgtgcga gtcacgcgtg cgtttgagcc
     1021 ggcgggccac cggcgtgttt cccatgagca cgtctatgga attatccatg acgcttagca
     1081 cctcgacccg atccacaggt ttgagtggaa tgacagccat cagaacctcc gatcttgtga
     1141 ggacgctaac ggaaaccggt gatacccgtc aaggccaaac aaagcgaaat ggcgcagatt
     1201 gttcgaaggc agcacgggat atgcgtagac tctgtgcgcc agcaattttt cagccgcgcc
     1261 tatattgcat tttctgtttc tacattgaga gattggcctt cgctgtctca atcagcagat
     1321 gggcgtaggc gccaagccgg ccgtagactg actatcgcag cgggtattca gtgatagcta
     1381 gtgccaaaca cctttttacc ttgcgctgtc catccttgtc gacatcgcca atcggtttta
     1441 cggttgacca gggaaacaat tactaggcca gagtgagatt ctcggagcat tgtcacgagc
     1501 acaagaattc gacaagatgg agcacgcgcc tgaaagggga tcttgcaatg cagttgttgc
     1561 tgatcgtggc gttagggcta gccattttaa ctgtcatttt tgcgctgaaa aatcctatcc
     1621 gcgttggtgt aacgtttctt ctttgaaatt tgaaggctcg ttggcgttgg ttctgatatc
     1681 aacatttgca ctaacagggt gctgaaaaaa gctcctgcag gctgctcaaa aaagatccag
     1741 gtgcgaggcg cgcgaaaaat cgacgagcgg aggcgtactt acagtacgtt gcagcgagac
     1801 gattgagcgg caacgaagca tatgggtctt tttcagcaac ctgctaggag ttttagtgag
     1861 tttgttgcta tccattcctg ctatagtaaa aagaagatcg gcaattgcga atcagagcaa
     1921 gagaatagcg cagctcgaga atcgctcacg ggaacggatc ccaccgtctt gatgaaagct
     1981 gcgctacggg agttgtattt tggtctgttc acctcctgag acaacttccg gctgatccgt
     2041 cacgccgtac gaccgtcgat tcactctcat acgaatgacg attgttctag gggcggaggt
     2101 atctcagcgc aggcacagcc gtgtcagtac tgatcgctga tctaggatag gataaggcaa
     2161 atgcggcgca caaaaattat ttgtaccatc ggtcctgcta cagagtcggc tgagatgctt
     2221 cgcaagttgg tggaggccgg taccgatgtc tttcgtctca acttctccca cggtacggta
     2281 agcggacacg agtccatcat acgccgcatc cgggacgtcg cggaggagac cggaaagccc
     2341 gttgccatcc ttcaggacct acccggtccc aaaatccggc ttggcgtttt caaagatgga
     2401 tcggtcgagc ttcatgccgg agatcgattc cgcctcacaa ccgatgaagt tcccggagat
     2461 catgaatgcg catcggtcag ctattccaaa cttcctgacg aagtgaggcc aggtcaaagc
     2521 attctacttg ccgacgggac tgtggagctc gaggtcgaat cagccgcccc acggaagatt
     2581 gtttgccgag tcgttctcgg tggagtcctt acgaatcaca aaggcgtcaa tgtggtaggg
     2641 ggaagctcaa gcttacccgc cttcaccgag cgtgaccaga aacttcttct gtccggcctt
     2701 gaagcaagtg tggatctggt tgcactctcc tttgtccgct ctccggttga tattcagtct
     2761 gcccgaacct tccttgcagc ccaccaagca aaccttcccc taatggccaa gatcgagaag
     2821 ctcgagtcgt tggacattat agacgaaatt cttgctgtcg ctgattccat catggtagcg
     2881 cgcggcgatc ttggtcttga agttccgatt gctcaggtgc ctcacgtcca gaaggagcta
     2941 atctccaaag cgattcaggc cgccaaaccc gtggtgacag ccacgcagat gctccgctcg
     3001 atggtcgaaa accctcgacc cacccgtgcg gagatagccg acatcgctaa cgcggtactc
     3061 gacggttccg atgctttgat gctttcagag gaaagcgccg taggcgccta tccggtggag
     3121 gcagtcaaag ttctggcaga aactgccgag gtagccgaga cccggctttt tgcccggcga
     3181 cccttcctat gcctttctcc taccgaccgc gtttccgccg ctgaagctat tgcacatgcc
     3241 gcaagccttg tggcccatca ggctaaagca acagccatca tatgctgcac ccggacaggc
     3301 cagacggctc ggctcgttgc caaataccga ccggtccaac ccattatagc catgacccat
     3361 gaaccccaaa ccgtgcggcc gctcatgatt gtctggggtg tccagccatt cctgctagat
     3421 gaatgcagat cgattgacgc aacgataaag gccgcaatcg atcttgcttc caaaaaaaag
     3481 attgtgaagc ccggaaaagt agtgatcgtg gcggccgccc caaactctcc ccgcggagaa
     3541 accgatttcc tccgtgttgc taatctgaaa tgatcttgcg tgacccgagt caccgctgaa
     3601 atctctccca agcttattgt aagggaaatc cgtcgaatac gccggagatc ccttcctctt
     3661 aagatgtcgt ctctattcgc tagcggcgtt gatgttgcta ctgcgccgtc tcgaagccag
     3721 aagatatcaa gctggctttg gcgcggtcaa aacccagtct tccggtttca cctcctcttt
     3781 cccccgccat gaaagactca gaatattctt tatggcgtag gtgaccgcca gcggaaggaa
     3841 tctaattgcc tcggaatggc gcgagagtga aacggctttt cttacttctg ggtcggaaag
     3901 aacaaggcgg gctgcgttcg tgcctacttt aatgacgtat aagcatggcc gtaagagaat
     3961 agccatttgt tccagcgcga gtgctctaga ggcatgccct ttgcctactg ctatgttcct
     4021 gcaacagatt tg
//
LOCUS       DASUTG010000543         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1268615, whole genome shotgun sequence.
ACCESSION   DASUTG010000543 DASUTG010000000
VERSION     DASUTG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268615"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1427)
                     /locus_tag="VF089_12505"
     CDS             complement(210..1427)
                     /locus_tag="VF089_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6174830.1"
                     /translation="MPIFLWEGKTAQGRLMKGDLEAPNLEAVLSALRERRIRPIPNRI
                     RQKGQGLDKEISIPGFGEKVKPKEVSLFTRQFATMIDAGLPIVQCLDILSEQSASKLL
                     RNTIRGIRQDVEGGLTLAASLKKYPKVFDDLYVNMVEAGEAGGVLNTILNRIALFIEK
                     ATKLKKKVKGAMIYPCSILAVAIIVVAVLLIFVIPVFAELFTSMGKALPVPTQIAINL
                     SNWFIAYWLYMIFGMAAVIGTLRFYYQTPHGRMHIDGILLRMPIFGDLLRKVAVARFS
                     QNMSILLSSGVPILDGLAITARTAGNKVVEKAIMDSRLSISQGKTVAEPLRDSKIFPP
                     MVCQMVAVGESTGGLDGMLKKVGELYEEEVDDAVANLTALMEPLIMVVLGVILGALVI
                     SMYLPIFQMGSLV"
     gene            complement(1498..3225)
                     /gene="pilB"
                     /locus_tag="VF089_12510"
     CDS             complement(1498..3225)
                     /gene="pilB"
                     /locus_tag="VF089_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX6174831.1"
                     /translation="MEPRVAELLVRGGVVSRDQLNKAQQNGRDSGSSVMKELVQLGFT
                     TEQTLTEFLAKQFGIEKIELAPEEIEESVFTLVPPQLTQKHQLVPTKLTGSILTVAMA
                     DPTDLIAINEIKFITGYGVRVVLASPSAIKKTLDHRFGGISYDDVLKKFGDSDMELIL
                     EQDDVNLQELQQATMEAPVVTLVNAILADAAKRRASDIHIEPYEKLFRVRFRIDGVLQ
                     EIMSPPLRLKNPLVSRLKVMAGLDIAERRLTQDGRIKLKMGIGGELDIRVSILPTLFG
                     EKVVMRLLDKSNLQLDMAKLGFDPQTLQDFQEAIHKPFGMILITGPTGSGKSTTLYSA
                     LSELNKADVNISTAEDPVEYNLVGINQVQVREQIGLNFASCLRSFLRQDPDIIMVGEI
                     RDLETAQIAVKAALTGHLVLSTLHTNDAPSTIDRLINMGVEPFLLTSSINLIAAQRLV
                     RRICDKCKEPIEVTPEALINLGVDASEAAGGFPTFRGRGCNSCNDTGYRGRLAIYEIL
                     VMHEGLKELILKSASAADLKREAVKLGMSTLRMSALQKVRDGFTTIEETIRVTDTDKG
                     FGSVFSLGF"
     gene            complement(3362..>5223)
                     /gene="ppdK"
                     /locus_tag="VF089_12515"
     CDS             complement(3362..>5223)
                     /gene="ppdK"
                     /locus_tag="VF089_12515"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX6174832.1"
                     /translation="NAQGEDVVAGVRTPKAIAELKDLMPKPYAQLKRVCRTLEKHYGD
                     VQDIEFTIENNNLWMLQTRSAKRTGFAAVRVAVDMVDEKIITKKEALLRIEADHLNQL
                     LRPVFDRHDKHKAALEQHVLVKGLPAGPGAATGRIVFHAEDAVEWSRSGERVILCRIE
                     TSPDDIRGMEVAEGILTARGGMTSHAALVARQMGKVCVAGCEALHIDYITRVMRVNGT
                     TLNEGDWISVDGSTGEVIKGEVQTRQSEVLEALLENNVQASKSPLYRRYSKLMKWADA
                     FRRLGVRTNTDQPDQAKVARAFGAEGIGLCRTEHMFFQGDRIQAVREMILADDEAGRR
                     KALAKLLPMQKEDFKAILEIMGDLPVAIRTLDPPLHEFLPSREEEIVELAHVMSVPLQ
                     SLQDKVKILQEFNPMLGHRGCRLGISYPEITEMQARAIFEAACELRQKGKRPFPEVMI
                     PLVGSREEFTAQKNIVQTVAEETMKVYGVKVRFLIGTMIELPRACLVADEIAQEAEFF
                     SFGTNDLTQTCLGLSRDDAGKFLPTYVLDGLLPEDPFVSVDQDGVGTLMKIAVEKGRR
                     ARKNLEIGICGEHGGDPKSIHFCHRIGLDYVSCSPYRIAIAKLAAAQASLRKT"
BASE COUNT         1263 a         1399 c         1224 g         1337 t
ORIGIN      
        1 tcttagtata atgaagaaaa accgcggatt cccttccgct tgaaaaaagc tgaacagggc
       61 cgtcgccgct aaaaaaaacg ttagaatggc tagtcgaagg agaagcagcc atctgatctt
      121 ttcagttaaa cttttattat cgtcagtcaa aagaagttca gatgggtctc aggtgtgcag
      181 atcttctgag aggtttcgct tattcacgac taaaccaagg atcccatctg gaagataggt
      241 agatacatgg aaatgaccaa tgcgccgaga atcacaccca acacaaccat gatcaatggc
      301 tccataagtg cggtcaaatt ggcgacggcg tcatcaacct cttcctcata cagttcgccg
      361 acctttttca gcataccgtc gagaccgccg gtgctctcgc ccacagccac catctggcag
      421 accatgggag ggaagatttt gctatctcgc aaaggttccg cgacggtttt accttgactg
      481 atacttagac gactatccat aatcgccttc tcaaccactt tgttaccagc tgttctagcg
      541 gtgatcgcca agccatcgag aataggtaca ccggaactta gaaggataga catgttttgc
      601 gaaaatcttg ctacggcaac tttccgcaat agatcgccga aaatcggcat ccggagcagt
      661 attccatcaa tgtgcattct gccgtgtggt gtctgataat agaaccgaag cgtgccgatt
      721 acggccgcca ttccgaaaat catgtagagc cagtatgcaa tgaaccaatt actaagattg
      781 atggcgatct gtgtcgggac cggcagcgcc tttcccatac tagtgaacag ttcagcaaaa
      841 accgggatga cgaagatcag taataccgcc acgacgatga tcgccaccgc cagaatcgaa
      901 catggataaa tcatcgcgcc tttaactttt ttctttagct tggtggcctt ttcgatgaac
      961 agagcgattc tgttcaggat cgtgtttaga acaccaccgg cttcgccagc ttccaccatg
     1021 ttgacatata ggtcgtcaaa aactttggga tacttcttaa gagaagctgc caaagtcaaa
     1081 cctccctcaa catcttgcct aattccgcga atcgtattgc ggagcaattt actagctgat
     1141 tgctcgctaa gaatgtccaa gcactgaaca atcggcaaac ccgcgtcgat catcgtggca
     1201 aattgacgcg tgaataaaga cacctcctta ggttttactt tctcgccaaa acccgggatg
     1261 ctaatttctt tgtcgagacc ctgccccttt tgccttatcc gattcggaat cggccgaatc
     1321 cgccgctcgc gtaaagccga aagtacggcc tcaagattgg gagcctcaag atcccctttc
     1381 atgagccttc cctgggcagt ttttccttcc cacagaaaaa tcggcatggg ttgttatcct
     1441 tcgctacttt cggctgtggc tgaacggcag catgtgacgg acgcaggctg cgtgctgtta
     1501 aaatcctaat gaaaaaactg acccgaagcc tttgtctgta tcggtaacgc ggatggtctc
     1561 ctctattgtt gtgaaaccgt cccgaacctt ttgcagcgca ctcatacgga gcgtactcat
     1621 gcccaactta accgcctctc ttttcagatc ggcagccgaa gcagacttca atatcaattc
     1681 tttgaggccc tcatgcatga cgaggatttc gtaaattgcc agtcggcctc gataaccggt
     1741 atcgttgcag ctattacagc ctcgcccgcg aaaagttgga aaccctcctg cagcctcgga
     1801 cgcatctaca cccaaattga tcaatgcctc cggcgtgacc tcgatcggtt ctttgcattt
     1861 atcgcaaatg cgccgcacca agcgctgcgc tgcgattaag ttgattgacg aagtcagtag
     1921 aaacggctcc acgcccatat tgataagcct atcaatggtc gatggagcat cgttagtatg
     1981 cagggtgctc aggacaagat gacccgtcaa agcggcttta acggcaatct gcgcagtctc
     2041 aagatcgcga atttcaccga ccatgatgat atccgggtcc tgccgaagaa aagagcggag
     2101 acatgaagcg aagttaagtc ctatctgctc tcggacctga acttggttga tacctacaag
     2161 attgtattcg accgggtcct cagcggttga tatattcacg tcggctttgt tcaattctga
     2221 aagtgcggag tagagtgtgg tcgacttacc gctgcctgta gggcccgtta taaggatcat
     2281 gccgaacggt ttatgaatcg cttcctggaa atcttgcagc gtctgaggat caaagccaag
     2341 cttggccata tcgagctgga gattggactt gtcaagcaag cgcatcacga ctttttcgcc
     2401 gaagagggtg ggcaaaatag atacccggat gtccagttcg ccgccaatac ccatctttag
     2461 cttgatgcga ccatcttgtg taagtcgcct ttccgcaatg tcgagcccgg ccatcacctt
     2521 gaggcgagaa actaatggat ttttgagccg gagcggcgga ctcattatct cttgaagcac
     2581 cccatcgatg cggaatctca cacggaaaag cttttcgtag ggctcgatgt gaatgtcact
     2641 cgctctgcgc tttgccgcat cggctaagat ggcgttgacc agagtgacta ctggtgcttc
     2701 catcgtagcc tgttgcagct cttgaaggtt gacatcatcc tgctctagga tgagttccat
     2761 gtcactatcg ccgaatttct tcagaacatc gtcgtaactg ataccgccaa atcggtgatc
     2821 tagcgttttc ttgatggcgg acggtgatgc tagtacgact ctcactccat agccggtgat
     2881 gaatttaatc tcatttatcg ctatcagatc cgttgggtca gccatcgcga cggtgagaat
     2941 cgagccggtg agcttggttg gaactagctg gtgtttctgc gtaagctgcg gcggtaccag
     3001 agtaaaaact gactcctcta tttcctcggg agccagctcg atcttttcga tgccaaactg
     3061 tttcgccagg aattcggtaa gtgtttgttc tgtcgtaaat ccgagttgga caagctcctt
     3121 catgacggat gaaccgctgt cacgtccgtt ttgctgggcc ttgttcagtt ggtcgcgtga
     3181 aacaaccccc ccgcggacca acaattctgc taccctaggt tccatagatc acttgaagaa
     3241 agattctgaa cgaaattcgg ctcgtgaaat tatggtgaat aaatacagac ctgtcaatca
     3301 aacttctccc gaggagagca gcgtattcag gagtgaatta ccggctaagc atctaaaata
     3361 actaggtttt tcgcagggac gcctgagcgg ccgctagttt tgcgatagcg atacggtagg
     3421 gcgaacagct gacatagtcc agaccgattc gatgacaaaa atggattgat ttgggatcgc
     3481 caccatgttc accgcagatt cctatttcaa ggtttttgcg tgcccttcgc cccttttcca
     3541 cggcaatctt catcagagtc ccgacaccgt cctgatccac gctaacaaac ggatcctccg
     3601 gcagaagccc atcgagcaca taagtaggca aaaatttccc ggcatcgtca cgtgaaaggc
     3661 ctaggcacgt ctgtgtaaga tcattggtgc caaaagaaaa aaattccgct tcctgtgcga
     3721 tctcatccgc gaccaagcat gcccgcggca actcaatcat agtgcctatg agaaagcgga
     3781 ctttcacgcc gtagaccttc atcgtttcct cagcgacagt ttgaacgatg ttcttttgcg
     3841 ccgtgaattc ttcacgcgac ccgacaagcg gaatcatcac ctcaggaaaa ggtctctttc
     3901 ctttttgacg caattcacac gctgcctcga atattgcgcg ggcttgcatt tccgtgatct
     3961 ccggatatga aattcccaag cggcagcctc gatggcccag catgggatta aactcttgaa
     4021 gaattttgac tttgtcttgt agagattgca gcggcacgct catgacatgc gccaactcca
     4081 caatttcttc ctctcggctg ggcaggaact catgtagcgg cggatcgagt gtccgaatag
     4141 cgacaggcaa atctcccatg atttccaaaa tcgctttaaa atcctccttt tgcatcggca
     4201 aaagcttagc caatgccttt cggcgtcccg cttcatcatc cgccaggatc atctcgcgca
     4261 cggcttgaat ccgatcgcct tgaaaaaaca tgtgctcggt tcgacataag ccgatacctt
     4321 cagccccgaa ggcacgcgca accttggcct gatccggctg atcggtattc gttcgcaccc
     4381 ctaacctgcg aaatgcgtct gcccacttca taagctttga gtaccgacgg tacaagggag
     4441 atttgctcgc ctgcacgttg ttttctagga gcgcttcaag gacttccgat tgccgagtct
     4501 gaacctcacc tttgataact tcgcctgtgc ttccgtctac ggagatccaa tctccttcat
     4561 tgagagtcgt tccattcact cgcatcactc gcgtgatata atctatatgc aacgcttcgc
     4621 agccggcgac gcatactttg cccatctgcc gtgccactag tgccgcgtgc gaagtcatgc
     4681 cgccgcgggc ggttagaatt ccttccgcca cctccatccc gcgaatgtca tcggggctcg
     4741 tttctatgcg acagagtatt acgcgttcgc cacttctcga ccattccact gcatcctctg
     4801 catgaaagac gatgcgacca gtagccgcgc ccggccccgc cggcaaaccc ttgaccagaa
     4861 cgtgctgttc tagcgctgcc ttgtgcttgt catggcgatc aaagactggg cgaagaagct
     4921 gatttaaatg atccgcttcg attcgaagca aagcttcctt tttggtaata atcttttcat
     4981 caaccatgtc cactgcaact cggacagcgg cgaatccagt gcgtttggcg gagcgggttt
     5041 gaagcatcca caggttatta ttttcgattg taaattcgat gtcctgcacg tccccgtaat
     5101 gtttttctaa tgttctgcag actcttttca gttgcgcata gggctttggc attaggtctt
     5161 tcaattcggc gatggcttta ggtgttcgaa cacctgcaac gacatcttca ccctgtgcat
     5221 tga
//
LOCUS       DASUTG010000544         9586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_974199, whole genome shotgun sequence.
ACCESSION   DASUTG010000544 DASUTG010000000
VERSION     DASUTG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974199"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VF089_12520"
     CDS             complement(<1..839)
                     /locus_tag="VF089_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174833.1"
                     /translation="MGHLSILSRAEVETLGPPTPEELGETGIHDAFLCDLTLKHVAML
                     PEPTTASVADRLHLPRALTEELLYHLYREKLIEMRLQSAVGNTRYAMLDHGWEKVSRL
                     QSQSGYLGPAPVSLDDYAHMMKLQAVPSRPAYMEAVRNAFRDLILPDSLLQTLGCVIN
                     SRSSLFLTGLPGTGKTAVSERMNAALFGHIWIPYAVEIDGQIIRIFDAHCHRAVPPRE
                     TALEHDRRWVEIQRPLVIVAGELTLDNTDLAWSQSAKFYEAPFQVKSNGGTLVIDDFG
                     RQRV"
     gene            complement(886..1803)
                     /locus_tag="VF089_12525"
     CDS             complement(886..1803)
                     /locus_tag="VF089_12525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174834.1"
                     /translation="MTEAEMAMMNAPIGNDKEKRGNAFVRVLKAPFKAIGRLFRRGRK
                     DDNKLHRLSEKDVKKFETAPVTRVLNAESIPTTTNVAPSAPPAETLSQAVDDRKSLAL
                     EHLERGRQLLHTGKVNDAITALSTATSFDPKLKEAYNLLGVAYGTKGMRNLALGSFER
                     ALEGDDDNPEHLNNLGYFLYQIGELEDATKYLKKAVKKTPTNERFWNNLGLVQAQRGK
                     FDDASKSFVKAMGEFDGRLNLAVRLERLGHEKEAIKNLEKARKLRPNSQETLSRLITL
                     YERVGKDEEAMEMRISLVSLKATAKTPAE"
     gene            complement(1901..2845)
                     /locus_tag="VF089_12530"
     CDS             complement(1901..2845)
                     /locus_tag="VF089_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6174835.1"
                     /translation="MLIISLSVFVCIAFGVMAIYWLMFRPASATADRLRELDDSRVAG
                     PTQTIEHNPVTTLAERLAEPLGRLVPPSAANAKKLQKDLMQAGFRSHYATSVYRVMQL
                     LSMVVCPGFILLVWMYLAKPMSEAMMPAIFALGAGFILPRFMLNRMMASRKLRITWGL
                     ADALDLMVITMEAGLGLNAAMLKVCEELKDVHPDISKEFEMANLEIRVGRERSEALRN
                     LADRSGVEDLNSLVGMLIQADKFGTSIARAVRVYSESLRTKRRQRAEQAAQKAAFKLL
                     LPLGGLLFPTMFIIILGPALLNISDMLGGGVSNSLNLK"
     gene            complement(2880..3848)
                     /locus_tag="VF089_12535"
     CDS             complement(2880..3848)
                     /locus_tag="VF089_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6174836.1"
                     /translation="MLLVPFFVFLSCLFIVYALYVLSSRGSDARRARLDERLAEAIRS
                     SAHSNDVDVQLAREELLSEIPWMNRVLIKLEFTSRMKHIIDQADSQITVMRLVLLSLT
                     AGVLAVLAVSMVSASYLLMVLCGIIATAVPFLHIAMKRKKRLKKFLQLLPDALDLMSR
                     GLSAGHAFTESLQMVASEMPEPIATEFRKTYDEQNLGLSLKLALENLVQRMPLLDLRM
                     CVTAVLIQRETGGNLSELLEKVAYTIRERFRIMEDLKTLTLSSRWSAWLLCALPIFMA
                     VYITAMNPNYMEVMWRDPRGHKLLAIAVIMQILGMLMVQKIMKIKI"
     gene            complement(3853..5187)
                     /locus_tag="VF089_12540"
     CDS             complement(3853..5187)
                     /locus_tag="VF089_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6174837.1"
                     /translation="MALRERLVRDHATGSLISDDLGSNQQGFQDMKSRLHRMLINRMD
                     LTKLGMMEPEQLHEEVARLAASLLAQEDTPLSTVERERLIHEVRHELFGLGPLEQLLA
                     DTTISDILVNSPYNIYIERRGKLERTNIEFKDNEHLMRVIERIVSSVGRRIDESSPMV
                     DARLKDGSRVNAIIPPLSLDGPVLSIRRFGAEPLRMHKLIENDALTPDMAQMFEMCVH
                     ARLNILISGGTGAGKTTLLNALSAYIPEDERIVTIEDSAELQMQQPHVVRLETRPANI
                     EGKGEVSQRDLVKNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTVHANTSRD
                     ALARLETMIQMTGMRLSDRAMRQQIASALNLVIQVARLSDGTRRIIAISEITGMEGET
                     ITMQDVFVYERKGIDKDGKVVGRFRPTGVRPRFAERLKVYGMQMPRSFFEEV"
     gene            complement(5231..6319)
                     /locus_tag="VF089_12545"
     CDS             complement(5231..6319)
                     /locus_tag="VF089_12545"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174838.1"
                     /translation="MIINLSHMGEPALKLVQRITAECPTTAVICASRDSSPDLILRSM
                     RSGARDFLRLPIIEEELTTVIDRTAAFTAEHIDDEPQKRGQAIAVFSSKGGCGCSLIA
                     TNLAIVQTAPTVLVDLNLQSGDLELLLGVRPKFSLADVIENRERLDDALLRSYLTEHS
                     KHVSLLAAPVKAESAEDIEPKHIYEVMEMLRQRFDSVIIDTPHTFDALTISALDHADQ
                     ILVVLTLEIHAIRSTRRALEIFDRLGYPRKKIRLVVNRWSKNIDLNQKQVEDFLGERV
                     VGFIQSDYRAAVNSINMGQPLVTSAPTSRVAADLREMAAKLFEGKVDGVAPVAESKRS
                     FNSMFRRQPVTSVEDFGLSAALNKASIS"
     gene            complement(6522..7040)
                     /locus_tag="VF089_12550"
     CDS             complement(6522..7040)
                     /locus_tag="VF089_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6174839.1"
                     /translation="MRNYHTRDNERGSTMAEFAVVAGLFFMMIISVIEFSRLLYTHNA
                     LTDAARRGARFAALHKEADETCARNVVIYGENHVDMNSGCAPTGPALVNGIEAADINI
                     IYQGADTDNDVSDPTPIDTSYGTNLGTVTVTIENYQFNLQVPYFQVGIDLPDYTTTIT
                     AESAGEEPEALP"
     gene            complement(7068..7526)
                     /locus_tag="VF089_12555"
     CDS             complement(7068..7526)
                     /locus_tag="VF089_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6174840.1"
                     /translation="MAELAIVLPFLIVMVATTAEVGRFFQSYTTLSKSTRSAARYLSN
                     HQFNNLEKDRAKNLVVCGKLSCATGEELVKGLSTSNVCIQTTLTPSNTVETITVSLPR
                     TTSMTCEDGGTATQLLFDPIFNIGALLNNDTFDLAYPLAPSTTMRYIPAD"
     gene            complement(7600..9060)
                     /locus_tag="VF089_12560"
     CDS             complement(7600..9060)
                     /locus_tag="VF089_12560"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6174841.1"
                     /translation="MENKKVACNDKWKNQRGGVLAYTVLSALALFLAVGLGVDLAHLY
                     TVKTELQNAADSAALAGASALDLPADAKIPESADRAVQILNNNKYNFNNKTFVSGTPD
                     LATLRTQLRPYLRYAVNLSEFDNGGMGKTYEEAEADPDDIRFIQVITPNAPVDIYFAL
                     PILGGQRNLTARAVAGYSVPGNTEFCIFPLSAVSCDPGDANCKLECRKTDDPSTPEID
                     ESEPGCKCDPSAADYPQPECTKYYGECPNNANYAPIPVADGGLDPDGDGFCDPKREFC
                     KGCTYTVRSEPAKGPAAGSYNILKCGDGGKCETRMALAAYGSCDCKASAGDPLSTDNE
                     PGQAAGPVRQGINVRFDTYNGCDPATDPTCDPGCQPNPTDHPPDSNIAEPINYGQYSA
                     NPPNPFLAPPDHPPGVAQRRVVVMPIVPLGEYTDGNGRQVVHPSGFAGFFIQKRVPNG
                     SGDIKIEYMAEDIVDVIGFDPNSTNVTNVVTPVLYR"
     gene            complement(9181..>9586)
                     /locus_tag="VF089_12565"
     CDS             complement(9181..>9586)
                     /locus_tag="VF089_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174842.1"
                     /translation="NNETRSLSKIPVLGDIPILGNLFKSSQFQKQETELMFIITADLV
                     KPVNRDDIPQMKGVDGLKNGSPLGIEPKGDGINGKTGFSTGAADSKEAEAKPVTTSAA
                     QPDAQTKPVASDEKTVKAAALVVPPASKATPE"
BASE COUNT         2134 a         2565 c         2559 g         2328 t
ORIGIN      
        1 acgcgttgcc gcccgaaatc atcaatcacc agcgtgccgc cgtttgactt cacctggaaa
       61 ggcgcctcgt aaaactttgc agactgcgac catgcgagat ccgtattgtc gagtgtcaac
      121 tcacccgcca cgatcaccag cggcctttga atctcgaccc atcgacgatc atgttcgagg
      181 gcagtttctc gcggggggac cgctctgtga cagtgggcgt caaagatccg aatgatctgg
      241 ccgtcgattt ccacggcata ggggatccag atatgcccaa acaacgctgc gttcattcgc
      301 tcagatacgg cagtctttcc cgtacccgga agtcccgtca aaaacaggga tgaccgtgaa
      361 ttgatgacac agcccagtgt ttgcagtagg gagtccggga gtatcaaatc acgaaaagcg
      421 ttccgtactg cctccatata agccggacgg gacggcaccg cctgcagctt catcatgtgt
      481 gcgtagtcgt cgagagaaac tggcgccgga ccaagatagc cgctctgaga ctgcaatcga
      541 cttacctttt cccaaccgtg atcgagcatt gcatagcgag tgttgccaac cgcgctttgc
      601 agtcgcatct cgatcaactt ttcacggtag aggtgataga gcaattcttc agtgagcgcg
      661 cgcggaagat gtagcctgtc agcaacggaa gccgtggtcg gctcgggaag catcgcaaca
      721 tgctttagag tcagatcaca cagaaacgca tcgtgaattc cagtctcgcc taactcttcc
      781 ggagtgggcg gaccaagcgt ttctacttcc gcgcgactca aaatactgag atgtcccatg
      841 aggtgcctct tgattcttcc tcttcccttt tctgcgagtg ataggttatt cagccggtgt
      901 tttcgctgtc gctttaaggc ttactagcga aatgcgcatt tccattgctt cctcatcctt
      961 gccgacacgc tcgtaaagtg tgattaaacg gctgagcgtc tcctgtgaat tcgggcgaag
     1021 cttgcgcgct ttctccaaat tcttgatggc ttctttttcg tggcccaaac gctcaagtcg
     1081 gacggccaaa ttaagtcggc catcgaattc tcccatcgcc ttcacgaagc ttttggaggc
     1141 atcatcaaac ttaccccgct gggcttggac cagaccgaga ttattccaga agcgttcgtt
     1201 agtcggagtt ttcttcacgg cctttttcaa atacttagta gcgtcttcca attcgccgat
     1261 ttgataaagg aagtagccga gattgttgag atgttcagga ttatcgtcat cgccttccag
     1321 cgctctctca aatgaaccaa gcgcaagatt gcgcatgccc ttggttccgt atgcgacccc
     1381 gagcaggttg taagcctctt tcaattttgg atcgaaagaa gttgccgtcg agagggcggt
     1441 gatggcgtcg tttaccttgc cggtgtgcaa caactgcctc ccgcgttcca gatgctccag
     1501 cgccaaagac tttcggtcgt ccaccgcctg cgagagcgtc tcggcaggcg gggcggacgg
     1561 ggccacgttt gtggtcgttg gtattgactc tgcgttgaga acgcgagtaa caggcgccgt
     1621 ctcaaacttt ttcacatctt tttccgaaag gcggtgaagc ttgttgtcgt ccttacgacc
     1681 acgacggaag aggcggccga tggctttgaa cggggccttc aagacgcgca caaaagcgtt
     1741 cccgcgtttt tccttatcgt tgccgatggg agcgttcatc attgccatct ccgcttccgt
     1801 catcagcata ctgcggacgg actgtgaagg gggaagggca acgaggagcg tgaaactcag
     1861 cgacagaaca attattgtgc gctttagcat gatgctactc ctatttaaga ttaaggctgt
     1921 tacttacgcc gcctcccaac atgtctgaaa tgttgagcag cgctggaccc aggatgatga
     1981 tgaacatggt tgggaagagt aagccgccca acggcagcag gagtttaaag gccgctttct
     2041 gggccgcttg ttcagcgcgc tgacgccgtt tcgtccgtaa actctcggaa tagacgcgga
     2101 cggcacgagc tatcgaagtg ccaaatttat ccgcctgaat gagcatgccg accaggctgt
     2161 tcagatcctc aacaccggaa cggtctgcaa ggttacgcag tgcttcgctg cgttcccgcc
     2221 ctacgcggat ttccagattg gccatctcaa actctttact aatgtccggg tgaacatcct
     2281 tcaactcttc acagaccttc aacattgcgg cgttcagtcc caaacccgct tccatggtga
     2341 tgaccatcaa gtcgagtgcg tccgcgaggc cccaggtgat gcgaagttta cggctcgcca
     2401 tcatccgatt gagcatgaat cgcggaagaa tgaatccggc gcccagcgcg aagatggctg
     2461 gcatcatcgc ctcgctcatc ggcttggcca gatacatcca aaccaacaga atgaaccctg
     2521 gacaaaccac catcgacagc agttgcatta cccgataaac cgaggtcgca tagtgggaac
     2581 ggaagccggc ctgcatcaag tccttctgca atttcttagc gttcgcggca gatggcggga
     2641 ccaaacgccc caaaggttcg gccaggcgct cagccaatgt cgttaccgga ttgtgttcaa
     2701 tcgtttgtgt agggccagcc actcgcgagt cgtccagttc tcggagtcga tctgccgtgg
     2761 cgctagccgg ccgaaacatg agccaataaa tggccatcac gccaaacgct atgcaaacaa
     2821 aaacggacaa ggaaataatt aacatgttgt cacctaccag aggatgctat accgaacagt
     2881 tagatcttga ttttcatgat tttctggacc atcaacatgc cgagaatctg catgatcact
     2941 gctatggcca gcagcttatg tccacgcggg tcgcgccaca ttacttccat gtaattcgga
     3001 ttcatcgccg taatgtatac ggccataaag attgggagag cgcacagaag ccaggcggac
     3061 caccgcgagc taagcgtcaa agttttgagg tcctccatga ttcgaaaacg ctcgcgaatg
     3121 gtgtacgcca ccttctcaag caattcagaa agattaccgc cggtttctcg ttggatcagc
     3181 acggcggtga cacacatacg caaatcgagc agcggcattc gctggaccag gttttcaagg
     3241 gccaatttca atgagagccc cagattctgt tcgtcataag ttttgcgaaa ctctgtggcg
     3301 atcggctcgg gcatttcgct ggcgaccatc tgaagcgact cggtaaacgc gtggcccgca
     3361 gagagcccgc ggctcataag atccagtgcg tcgggcaaca gttggagaaa cttcttcaac
     3421 cgtttcttac gcttcatagc aatgtgtaag aacggcaccg cggtcgcgat aattccgcaa
     3481 agaaccatta ataaatagga agcgctaacc atcgaaacag ccaacactgc aagtacgccg
     3541 gccgtgagcg agaggagaac cagacgcata acagttattt gcgaatctgc ctggtcgata
     3601 atgtgtttca tccgtgaagt gaattcgagc ttgatcaaga cccgattcat ccatgggatt
     3661 tcacttaaca gctcttcccg cgccaattgg acgtcgacgt cgttcgagtg cgccgatgat
     3721 cgaattgcct cggcgagacg ttcatccagg cgcgcccgtc gcgcatcaga cccgcgcgag
     3781 ctcaggacat agagcgcata aacgatgaac aggcaggaca aaaatacaaa aaacggaact
     3841 aaaagcatta tgctagacct cctcgaaaaa cgatcgcggc atctgcattc catagacctt
     3901 gaggcgttcg gcgaaacgcg gtcggacacc ggtaggacga aagcgcccga ccaccttccc
     3961 gtctttgtcg atacccttgc gctcgtagac aaaaacatcc tgcatagtga tggtctcgcc
     4021 ttccatgccc gtgatctctg aaatggcgat gattcggcgc gtgccatctg aaagccgggc
     4081 cacttggatt accagattca acgcggaggc aatctgctgg cgcatggccc gatctgatag
     4141 cctcatgcca gtcatctgga tcatcgtctc cagtctggcg agagcatccc tggaagtatt
     4201 ggcgtggacc gtggtgagcg aaccatcgtg accggtgttc atagcctgga gcatgtcgat
     4261 agcttcgcca cctctcacct caccgattac gattcggtcg ggccgcatgc gcagcgcatt
     4321 tttgacgagg tcccgctgac tgacttcgcc cttgccttca atatttgctg ggcgagtttc
     4381 cagccggacc acgtggggct gctgcatctg aagctccgca gaatcttcga tagtgacgat
     4441 acgttcgtct tccggaatat atgcagacag cgcattaaga agggtggttt tgccggcgcc
     4501 ggtgcctcct gaaataagaa tgttcagacg cgcatggacg cacatttcga acatctgggc
     4561 catatccggt gtcagagcgt cattttcaat caacttatgc attctcagtg gctcagcgcc
     4621 aaaccggcgg atagaaagca caggaccatc aagggagagc ggaggaatta ttgcgttaac
     4681 acgggagccg tccttcagcc gcgcatcgac catgggcgag gattcgtcga tgcggcggcc
     4741 gacggacgaa acgatgcgct cgataacacg catcaagtgt tcgttgtctt taaattcgat
     4801 gttggttcgt tcaagcttgc cgcgacgctc aatgtagatg ttgtagggag agttaaccag
     4861 aatatctgag atagttgtgt cagccagtaa ctgctcaagt ggccccagtc cgaatagctc
     4921 atgccttact tcatgaatta agcgttcccg ttcaacagtt gagaggggag tatcttcctg
     4981 ggccaacagg ctcgccgcca gccgagccac ttcctcgtgt agctgctcgg gctccatcat
     5041 cccgagcttc gtcaaatcca tccggttaat aagcatccga tggaggcggg atttcatgtc
     5101 ctggaaaccc tgctggttgc ttcccaggtc gtcgctaata agcgagccgg tagcgtggtc
     5161 tctgactagt cgttcgcgta aggccataag ttttacaacc cctctgcact actttcttct
     5221 ttatttacta ctagctgatg cttgctttgt tcagcgccgc gctaagtcca aaatcttcca
     5281 cggacgtgac gggttgacgt cgaaacatcg agttaaacga ccgtttgctt tcagcgaccg
     5341 gagcaacccc gtcgaccttg ccttcaaaaa gtttggcagc catctccctc agatcagcgg
     5401 caaccctgga agttggagca gatgtaacca aaggctggcc catatttata gaattgacgg
     5461 cggcccggta gtcactctgg atgaagccaa ctactcgctc gccgagaaag tcctcgacct
     5521 gtttctggtt gaggtcgatg ttcttgctcc aacggttaac cacgaggcgg attttctttc
     5581 gtgggtaccc gagccggtcg aatatctcga gtgcccgacg ggtactcctg atcgcgtgaa
     5641 tctcgagtgt caggacaacg agaatctggt ctgcatgatc cagcgccgag atagtcagtg
     5701 catcgaaagt atgaggtgta tctataatta cggagtcaaa acgttgccgc agcatctcca
     5761 taacttcata gatatgtttt ggttcaatat cctccgcgct ttctgccttg actggagcgg
     5821 ccaggagcga gacatgtttt gaatgctcgg tcagataact cctgagcagc gcatcatcaa
     5881 gtcgctcgcg gttctcaatg acatccgcga gagaaaactt gggccggact cccagcaaca
     5941 actccagatc gcccgattgc agattaaggt caaccagcac agttggcgcc gtttgcacga
     6001 tcgccagatt ggtggcgatc aacgagcaac cgcagccacc tttactggag aataccgcga
     6061 tggcctgtcc tcttttttgg ggttcgtcat caatatgttc ggccgtgaac gctgctgtac
     6121 gatcgatgac agtagttaat tcttcttcga taattgggag acgcaagaag tcccgcgcgc
     6181 cggaccgcat gctacgcaga atgaggtcag gggaagagtc tctggacgca caaatgaccg
     6241 cagtcgtcgg acactccgca gtaattcgtt ggaccaattt cagtgcgggc tcgcccatgt
     6301 gtgagaggtt gataatcacg gccgaaggac ggagtcgcac tgtttcggta aaaacctgct
     6361 cggcgtcatc gctcagcatc aacacgctgg cgccggcgtg attgcccaaa gctcggccta
     6421 attctttcga ttcggctgcg tccttacaaa ggattacgaa ggttaaggtt cttttcattg
     6481 cgaattcatt tccctgaata tgttccggat atcgcgagtt tttatggcag cgcctcgggc
     6541 tcttcgccgg cgctttcggc cgttatcgta gtggtgtaat cgggtaaatc aatccccact
     6601 tggaagtatg ggacctgcag attgaactgg taattttcaa tcgtcactgt gaccgtcccc
     6661 agatttgttc cgtaacttgt gtcgattggc gttggatcac taacgtcgtt atccgtatca
     6721 gcgccttggt atatgatgtt gatgtccgcc gcttcgatcc catttaccag ggctggccca
     6781 gttggagcgc atccgctgtt catatcgacg tgattttcac cgtagatgac cacattcctg
     6841 gcgcatgtct catctgcctc tttatgaagt gctgcgaacc gcgcaccgcg cctggccgcg
     6901 tccgtcagcg cattatgcgt atacagcaat cgtgagaatt cgatgacact gatgatcatc
     6961 atgaagaaca gcccagcaac tacggcgaat tccgccatag ttgaaccgcg ttcattgtca
     7021 cgagtgtggt agttgcgcat aaccttctca gtttctattc ccctcattca atctgccggg
     7081 atgtatcgca tcgtggttga aggggcgagg ggataggcaa gatcgaaagt atcgttgttt
     7141 aaaagcgcgc cgatgttgaa aattggatcg aaaagtagct gcgtggccgt gcctccgtct
     7201 tcgcaggtca tcgatgtcgt ccggggcaaa cttacggtaa tggtttctac ggtattgctt
     7261 ggggttaacg tcgtttgaat gcagacgttg ctagtcgaca aacccttgac gagttcctct
     7321 ccggtcgcgc agctcaactt gccgcagacg accagattct tggcccgatc tttttccaag
     7381 ttgttgaact gatgattgga aaggtagcgg gcggcggacc gcgtcgactt tgagagcgtg
     7441 gtgtagcttt gaaaaaaacg cccgacttct gcagttgtgg ccaccatcac gattaaaaat
     7501 ggcaggacaa tggcgagctc ggccaacgtg ccgccctgtt cattacgagc taggtggccc
     7561 aaatgcgaga ttattcttac tggctttttc atttcgtatc taccggtaga gaacaggtgt
     7621 cacgacgtta gtaacgtttg tggaattggg atcgaaaccg atcacgtcaa caatgtcctc
     7681 agccatgtat tcgatcttga tgtcgccgct cccgtttgga acccgctttt gaatgaagaa
     7741 gccggcgaag ccactggggt gaactacctg ccgcccattc ccatcggtat attcacccag
     7801 gggcacgatt ggcattacga ctactcgccg ctgcgcaacg cccggtggat gatctggtgg
     7861 cgcaaggaac gggttcggtg ggttggctga gtactgcccg tagttaatgg gttccgcgat
     7921 atttgaatcg ggtggatggt ccgtcggatt gggttgacat ccaggatcgc atgtcggatc
     7981 tgtcgcgggg tcacagccgt tgtaagtgtc aaagcggaca ttgatacctt gccggacggg
     8041 accggccgcc tgtcccggtt cgttatctgt ggacagagga tcgcccgcac tagctttgca
     8101 gtcgcaactg ccgtaggcgg cgagggccat tcgcgtctcg cactttccgc cgtcgccgca
     8161 cttgagaatg ttatagctgc cggccgccgg ccctttggct ggctccgagc gaacggtata
     8221 ggtgcagccc ttgcaaaact cgcgctttgg atcgcagaag ccgtcgccgt ccggatcaag
     8281 cccgccgtcc gcaaccggaa tgggtgcgta atttgcgttg ttagggcact caccgtagta
     8341 cttagtgcac tccggttggg ggtagtccgc agcgcttggg tcgcatttgc accccggctc
     8401 ggattcatct atctcaggcg ttgaggggtc atccgttttt ctgcattcca acttgcagtt
     8461 tgcatcgccc gggtcgcacg atacagccga cagcggaaag atgcagaatt cggtgttgcc
     8521 gggaacggaa taaccggcaa ctgcccgggc ggttaggttc cgctggccac ctagtattgg
     8581 cagggcgaag taaatgtcta caggcgcatt gggcgtaata acttgaataa agcggatgtc
     8641 gtctgggtct gcttcagcct cttcgtatgt cttgcccatt ccaccgttat caaattcaga
     8701 caggttaact gcatagcgaa ggtatggacg taattgtgta cgaagcgtcg cgaggtccgg
     8761 tgtgcctgat acgaaagttt tattgttgaa gttatattta ttgttgttaa gtatctgcac
     8821 cgcacggtcg gcggactcag gaattttggc gtcggctggg aggtccagcg cggacgcccc
     8881 ggctagcgcc gccgaatctg cagcgttttg cagttcggtt ttaacggtgt agaggtgcgc
     8941 aagatcaaca cccagtccta cggccaggaa tagagctaac gcagagagga ctgtgtaagc
     9001 gagtacgccg ccacgttggt tcttccactt atcattgcaa gcgaccttct tattttccat
     9061 cactggtact cctggaagat gtaaagtgca gctttttagc catatcgcta atgccgttga
     9121 gtggtagtca actcgtaaat gactaccacc cttaacagct gcttctcgaa ccaatacctt
     9181 ctattccggc gtggccttac tggctggcgg aaccaccagg gcagcggctt ttacggtctt
     9241 ctcatcgctg gccacgggct tcgtctgtgc gtcgggttgg gccgcggagg ttgttactgg
     9301 tttagcttcg gcttccttgc tgtctgctgc gccggttgaa aagcccgtct tgccattgat
     9361 cccgtctccc tttggttcaa tgccgagggg cgatccattc ttcaaaccat cgacaccctt
     9421 catttgtgga atgtcatccc gattcaccgg cttcacaaga tcggcggtaa tgataaacat
     9481 cagctccgtt tcttgttttt gaaactgtga ggatttgaag aggttcccga gaatgggaat
     9541 gtcgccgagc accggtatct tggaaaggga gcgcgtttcg ttgttg
//
LOCUS       DASUTG010000545         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_677631, whole genome shotgun sequence.
ACCESSION   DASUTG010000545 DASUTG010000000
VERSION     DASUTG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677631"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF089_12570"
     CDS             complement(<1..323)
                     /locus_tag="VF089_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX6174843.1"
                     /translation="MAKSPNDLVSFPIHLSREVERLFDEMIHRPWGFCREMRGWNPSV
                     DLYETDGAFMLEADLPGVKLDDVKVEIENSDLLLQGSRALEETHRHGHFHTMERSSGH
                     FIRRL"
     gene            580..936
                     /locus_tag="VF089_12575"
     CDS             580..936
                     /locus_tag="VF089_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174844.1"
                     /translation="MTESLQNMMGGDTSVTEQFFATLRRSEHLEPEKNLLIAILQDAI
                     HDYRKYCGARDPEGKEHFREAQKWIMAGGNNWIFSFNNICEFLGLDPDYIRRGLHDPD
                     ADRTKPRENRKIRDLH"
     gene            complement(1008..1814)
                     /locus_tag="VF089_12580"
     CDS             complement(1008..1814)
                     /locus_tag="VF089_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6174845.1"
                     /translation="MFDQVIVCLDGSSLAEKMIPLARALTRAAAGKLIFLRVVEDAAE
                     MSAEESYLKDCARHYDAELRFAVSPDPAVAIKTELEREPHAIAALTTHGRTAWTEAII
                     GSVAFRVLREGMRPVLLFRPHDRDSEPPTKITRITAALDGSAFSEKIIPYAARAARSL
                     SAHLLLVQALPVRRSPLTSDIHESDILESSYLHRLSAEIEVTHGIQAQWDVLHGETAD
                     ALCRYLKDMPDTLLAMTTHAHPAAKRVVLGSTAASCVRHAGVPLLLYWPQ"
     gene            2091..2576
                     /locus_tag="VF089_12585"
     CDS             2091..2576
                     /locus_tag="VF089_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6174846.1"
                     /translation="MAIINKLKELFEATKVSYEVYNHPLAYTAQEIAQRQHFSGNEMA
                     KVVMLKVDGKVVMAVVTGNQKVHLPTVRASLAAYEVSLATEDEFYSKFPGCEIGAMPP
                     FGNLYGLPVYVDPAVTHDESIYFNAGNHVQTVRMRYKDFESLVNPIVVRLTEEKRMHA
                     A"
     gene            2659..3048
                     /locus_tag="VF089_12590"
     CDS             2659..3048
                     /locus_tag="VF089_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174847.1"
                     /translation="MKWFLALIFLVASLAITGYLASDRSGIIETAQEQAVMLPPPDRS
                     GMMLVQTLGFFDEEVGAGLLLFYDDTQTNWQIDYIELYDSQDNLLLIAWLDRFGTCRA
                     VMDRGFLDTDNPNVDGVMVPIAVGTII"
     gene            3150..3563
                     /locus_tag="VF089_12595"
     CDS             3150..3563
                     /locus_tag="VF089_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174848.1"
                     /translation="MLLTISFLALPGNSCANFAGRVREYTYPPAFHYITDQQLRSTMW
                     QLAYHSRELRVLVPMKQETVQRDQVLHHLQAMEQVMIELNRTGWPTNHPMIDANLSTF
                     LRDIRSAGDAVSRDPPNFFLAGAISGACAYCHSNQ"
BASE COUNT          900 a          953 c          967 g          837 t
ORIGIN      
        1 ttcaggcgcc gtatgaaatg gcccgatgag cgctccatcg tatgaaagtg gccgtgacga
       61 tgggtttctt ccaacgcacg ggagccctgt aagagcaaat cgctgttttc gatctccacc
      121 tttacatcat cgagcttgac cccaggaaga tcagcttcca acatgaaagc accgtccgtt
      181 tcatagagat ctaccgatgg attccagccg cgcatctcac ggcaaaatcc ccaaggccgg
      241 tgaatcatct cgtcgaaaag gcgctccact tcgcgcgaga gatgaatcgg aaacgaaact
      301 aggtcgtttg ggcttttcgc catggccgcg atttctcctc attggtgcgg ctgcctactt
      361 caacgtcact aaaacgcaac ggccgtgcca cgtacacgct tgatttccct gccgctctgc
      421 agtcacaaga gagcggcgtt tcctaaacgt gggaagggtc gcgctgggtt tcttgcaacc
      481 ttgcgaagcg ggtcgcgccg ccactcaatc aattactggc acatcggttg cgttttcatc
      541 catctgaaac gggctcagcg cagaaaggac gctacgtcca tgacagagag tctccaaaat
      601 atgatgggcg gtgacacttc ggttacggaa caattcttcg ctactctgcg ccggagtgaa
      661 catctggagc cggaaaagaa cttgttgatc gcgattctgc aggatgcgat tcatgattat
      721 cgcaagtatt gcggggctcg agatccagag ggaaaggaac acttccgaga agcgcagaag
      781 tggatcatgg caggcggtaa caactggatt ttttctttca ataatatctg cgaattttta
      841 ggtctcgatc cggattacat tcgtcgtggg cttcacgacc cagatgcaga tcggacgaag
      901 ccgcgggaaa atcgtaaaat ccgagacctg cattaatcgt tgctgctcac ttcgactcat
      961 aaaaaccaag cctaccaatc actgctagca gtgcgcatca atcgagttca ctgcggccaa
     1021 tagagcaaca gaggcacccc tgcgtgccgc acgcaagatg ctgcggtgct acctaatacg
     1081 actcgcttcg ccgcgggatg ggcatgagtc gtcattgcca gcaacgtatc gggcatgtct
     1141 tttagatacc ggcagagtgc atcggccgtc tctccatgca gcacatccca ttgcgcttgg
     1201 attccatgcg tgacttcgat ctcggcggag agtcgatgca gataggacga ttcgaggata
     1261 tccgactcgt ggatgtctga tgttaggggt gaacggcgca ccggcagcgc ctgcaccaag
     1321 agcaggtgcg ctgataacga ccgagcggct ctggcggcgt acggaataat cttctcggaa
     1381 aaggcgctgc cgtcgagagc ggctgtaatc ctggtaattt tggtcggcgg ctcagagtct
     1441 ctatcgtgcg gccgaaacag gagcacgggt ctcatacctt cccgcaaaac ccggaaggca
     1501 acgctgccga tgatcgcttc ggtccatgca gtgcggccgt gagtcgtcag agccgcaatc
     1561 gcgtgcggct ctctttcgag ctccgtcttt atcgcaacgg ccggatctgg tgagacggca
     1621 aagcgcagtt cggcgtcgta gtggcgagca caatccttta aataactttc ttcggccgac
     1681 atttctgccg catcttcaac aacgcgcaag aatatgagct tgcccgccgc cgctcgagtt
     1741 aaggcgcgcg ccaaaggaat catcttctcc gcgagggatg aaccatcgag gcaaactatt
     1801 acttggtcga acatggtcgc cttcctcttt ttcgtgccgc agatatcaaa acggcaagag
     1861 ccgtaccaag tcagagtatt cgttggggag aaaaagaatt ggagtttgtt aaacaagtct
     1921 cgggcctctg acggtgtttc gccgaagcta cttttgcagt gataggaaac gaggcagtca
     1981 acttttcgcg cttgtgcgga acataagcgc tcttccccaa acaaaaacga ccacgaacgg
     2041 ggcacgattc ttgccctctt tgataatcac ggagttaaag gagaaacaaa atggcaatta
     2101 tcaacaaact taaagaactg ttcgaagcga cgaaggtcag ctacgaagtc tacaaccatc
     2161 cgctggcgta taccgcccag gagatcgctc agagacaaca cttctctggt aatgagatgg
     2221 ccaaggtcgt gatgctcaag gtggacggca aagtggtgat ggcggtggtt accggaaatc
     2281 aaaaagtgca cctgcctacc gtacgtgcaa gcctcgccgc ctatgaggtg agtttggcga
     2341 cggaagatga gttttattct aaattcccgg gctgtgaaat cggcgctatg ccgccgtttg
     2401 gaaatttgta tggtctccct gtctatgtcg atccagccgt cactcacgat gagtcgattt
     2461 atttcaacgc agggaatcac gtgcaaacgg ttcgaatgcg ctacaaggac ttcgagagcc
     2521 tggtaaatcc aatagtcgtt cggttaaccg aagagaaaag aatgcacgca gcctaaagtc
     2581 ggccggcagt tcgcatcgaa ttaccacaaa gagcggtttg tggctcccaa gcgccggcca
     2641 acaagcggaa ttccaaaggt gaaatggttt ctggcgttaa tcttcttagt agcgtcattg
     2701 gcgatcacgg gctacttggc cagtgatcgt tcggggatca ttgaaactgc gcaagaacaa
     2761 gctgtaatgc tgccgcctcc ggaccgcagc ggcatgatgc tcgtacagac gctaggcttt
     2821 tttgacgaag aggtcggcgc aggattgctc ctgttctatg atgacactca gaccaactgg
     2881 caaatcgatt acatcgagct atacgattcc caggataact tgctactcat agcctggctc
     2941 gatcgattcg gcacatgccg ggcggtgatg gaccgcggtt tccttgatac ggataaccca
     3001 aacgtcgatg gcgttatggt tcctatcgcc gtgggaacga ttatctgaac cataagacgg
     3061 atgcttgcgc tggctggcat gcatcacagc gcatcgcggc agggacaggc acaagaaagg
     3121 gaaataatga ggcagtgaaa aaatcattga tgttacttac tatctccttt ctcgcactgc
     3181 cagggaattc gtgcgccaac ttcgctggcc gggtgcgcga atacacgtat ccaccggcct
     3241 ttcactatat taccgaccaa caacttcggt ccaccatgtg gcaattagct tatcattcac
     3301 gagagcttcg ggtgctcgtt ccaatgaagc aggagacggt gcaacgtgac caggtgttac
     3361 atcatcttca agcgatggag caagtgatga ttgagttaaa cagaaccgga tggccgacta
     3421 atcacccgat gattgacgcc aatctgtcga ccttcttacg cgatattaga tccgcggggg
     3481 acgcggtcag ccgcgatcca cccaactttt ttctcgccgg agcaatatct ggcgcttgtg
     3541 catactgcca cagtaaccag tgagcataac ctgtgatcta ccaattatca ttgctaggag
     3601 actttcatcg gccaaattct cgattatgag aaagcatctg catgatcttg cattttt
//
LOCUS       DASUTG010000546         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1565331, whole genome shotgun sequence.
ACCESSION   DASUTG010000546 DASUTG010000000
VERSION     DASUTG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565331"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2067
                     /locus_tag="VF089_12600"
     CDS             <1..>2067
                     /locus_tag="VF089_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX6174849.1"
                     /translation="YKVGSASENYGETGMAHLLEHLVFKGTPRHPNIPQELTAHGARP
                     NGTTWTDRTNYFETFAATDENLNWALDLEADRMVNSFIRKEDLDSEMTVVRNEFELGE
                     NSPFGVLLNRVLSTAYLWHNYGKTTIGARSDLENVPIDRLQAFYKTYYQPDNAVLLIA
                     GKFDEQKTLGLVDKYFSPIPRPSRVLPKSYTAEPTQDGERSVTLRRVGDVQIVQAVYH
                     VPSGSHPDYAAIDILSQVFGHNPSGRLYKALVLEKKASAVFAFDFQWREPTLAILGAQ
                     VRQADSLDAAKDALLQTIESIGTNPPTKEEVERARAQLLKNIELALNNSDQIGLTLSE
                     FIGAGDWRLFFLHRDRLRKITTEEVAQAAAKYFKPSNRTLGLFIPTSKPDRAEIPPTP
                     DLAVALKDYKGDETVTAGEAFDPSPENIESRTIRTGPAGLKMALLPKKTRGGKVVAQM
                     TLRYGDEKSLMNRSTAAGLAGSMLIRGTTKRTRQQIQDELDRLKARLNVNGGATQANV
                     SIETTRENLPAVMRLAAEVLREPAFPAEEFDQLKRQNLAFIEESKSEPVQIAFTALSR
                     HLAPYPKEDVRYTRTVEENIAALNAATLEEAKQFYKDFYGASNATLTVIGDFDDKEIA
                     KLAIDLFADWKSPKTYVRIPTPFQDIAAVNQSFPAPDKANAFFVAGFNLKLRDDHPDY
                     AALLLG"
BASE COUNT          505 a          557 c          563 g          442 t
ORIGIN      
        1 ttacaaggtc ggctccgcct cggagaacta cggcgaaacc ggcatggccc acctgctgga
       61 acacctggtt ttcaaaggca ccccgcgtca cccgaatatt ccccaggaac ttaccgctca
      121 cggtgcgcgg ccaaacggca ccacctggac tgatcgaaca aactatttcg aaactttcgc
      181 cgctaccgat gaaaacctga actgggcttt ggacctcgaa gcggatcgca tggtgaattc
      241 ttttatcagg aaagaagatc tcgatagtga gatgacggtg gtccgaaatg agtttgaatt
      301 aggcgagaat agtccgttcg gagttttgct taaccgggtt ctgtcgaccg cctatctctg
      361 gcacaactac ggcaaaacga ctatcggagc gcggagcgac ctagagaatg tgcctatcga
      421 taggttgcaa gcgttttaca agacttacta ccagcccgac aacgctgtcc tgcttattgc
      481 cggcaagttt gacgaacaga aaaccctcgg actcgtagat aagtactttt ctcccattcc
      541 ccgtccgtca cgagttttgc cgaagagcta caccgctgaa ccgactcagg atggagagcg
      601 gtccgtcaca ctgcgacggg tgggagacgt gcagatagtc caggcggttt atcacgttcc
      661 ttcaggctca caccctgatt acgcggccat cgatatcttg tcccaggtgt ttggtcataa
      721 cccatctggt cgactgtaca aagcgctggt gctggagaaa aaagcgagcg cggtgttcgc
      781 gtttgatttt caatggcgcg agccgacgct ggcaattctc ggcgcgcaag taagacaagc
      841 cgattccctg gatgccgcta aggatgcgct gttgcagacg attgaatcga tcggcactaa
      901 ccctccgacc aaggaagaag tggaacgcgc ccgcgcacaa ctgctgaaga atattgaact
      961 cgccctcaat aattctgatc agataggact gacgttaagt gaattcatcg gcgcgggaga
     1021 ttggcgttta ttcttcctgc atcgcgaccg gctgcgcaaa atcacaacgg aagaagtagc
     1081 gcaggcggct gcaaagtatt tcaaaccatc gaaccgcacc ctcggtctct ttattccaac
     1141 ttccaagcct gatcgcgcgg agatcccgcc taccccggac ctggctgtcg ccttgaagga
     1201 ttacaaaggt gacgagactg taacggccgg tgaggcgttt gatccgtcgc cggagaacat
     1261 cgagtcgcgg acgattcgta ctggtccggc cggtttgaag atggcgttgc tgccgaagaa
     1321 gacacgcggc ggcaaggtcg tcgcccagat gacgctgcga tatggtgacg agaagagctt
     1381 gatgaaccgc tcgacggctg ccgggttggc tggttcaatg ctgattcgcg gcactacaaa
     1441 acgcacacgt caacagattc aggacgagct ggatcgcttg aaggcgcgct tgaacgtgaa
     1501 tggcggcgcc actcaggcaa atgtctctat cgagacaact cgagaaaatc taccggctgt
     1561 aatgcggctc gcggccgagg tgttgcgcga accggcattt ccggcagaag aattcgatca
     1621 gttgaagcgg cagaatcttg ctttcattga agaatcgaaa agtgagccgg tgcagattgc
     1681 ttttaccgcg ctgagccgcc atctcgcgcc ttatccgaaa gaagacgtac gctacaccag
     1741 gactgttgag gaaaatattg ccgccttaaa tgcggcgacg ctggaagaag ccaaacagtt
     1801 ttacaaagac ttctatggag cttccaacgc aaccctgacc gttattggcg acttcgacga
     1861 taaagagatt gcgaaactgg cgatcgattt gttcgccgat tggaagagcc cgaaaacgta
     1921 cgttcgcatt cccacgccct tccaggacat cgccgccgtg aaccaatcgt ttccggcgcc
     1981 cgacaaagcc aatgcgtttt ttgtagcggg attcaacctg aagctacgcg atgaccatcc
     2041 ggattacgcc gcgttgttgc tgggtaa
//
LOCUS       DASUTG010000547         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1565418, whole genome shotgun sequence.
ACCESSION   DASUTG010000547 DASUTG010000000
VERSION     DASUTG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565418"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..160
                     /locus_tag="VF089_12605"
     CDS             62..160
                     /locus_tag="VF089_12605"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174850.1"
                     /translation="MAWPNGADIAPETLYKAATVKADSNSKFRRAG"
     gene            2128..>2994
                     /locus_tag="VF089_12610"
     CDS             2128..>2994
                     /locus_tag="VF089_12610"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6174851.1"
                     /translation="MIRALLITANLVLILSSAAFAQDAKLIEAAKKEGGKVVVYGSLD
                     SDTGDAIGNAFLKKTGIKMDYWRASSTKVMDRATSEYRAGKPLFDVLINNASPMQILF
                     KEGIVGKYNSPSAKGFSKDVIDPDLGPRYRNVVIGIIYNKDTVKPADAPKSLEDLVKP
                     QYKGKVVMADPRQHTTTTQWLASLHKVMGQEKADKFIRDLAATKPALVESYIPAAERV
                     ITAETPIAIAFVRYVFLYGQKGAPLDYVRLGKMLGDGHHIAPSSKASNPNAAKAFIDF
                     FLGDESMELMTKI"
BASE COUNT          762 a          725 c          771 g          736 t
ORIGIN      
        1 cgattttcaa acctctgaaa aacaaaaact acttcaaaag ttttttgtcg atggtccaac
       61 catagcatgg cctaatggag ctgatattgc gccggaaacg ctatataagg cggcgactgt
      121 taaagcagat tcgaattcga aattcaggcg tgcaggttga gtggatgtcg agattcaaaa
      181 cgcgaccgca aggcgcgacc cccaatgcgc gtgatgggtt tccttgcgca tatgaacccg
      241 gtgcaggcgc gcagagttga gtctcgaagg gttacgagaa ggaatcagtc acaacaaagt
      301 aggcaatgtc ccgcgtgatt ttgtaacggg tcagaattca agcgcatatt tggtaatggt
      361 cgcgaaagca cttaacaccc tcggactaat gctcgacatt tcagagtgat tattctgttc
      421 tttttttcat ttccgcagcc ttcacaccaa gcatatgtcg gcatagctgt tgagtcaggc
      481 acggtgttat cggacggccg taccgccgca gatcatgcta acgatgcgcg tctgacgagg
      541 acagatacgt cttttactca ggttgcggca tgatgctgat catcgtcggc ttcgtattcc
      601 aacttattgg aacatgatct gatgagaaat ccgagtggct atagaactgc aagcggatgg
      661 ccattggcaa ctttttgacc cactcatcga tatcgtcaga ccgatttacc tagacatttg
      721 cccccagcga tgtgacaatc gattttaagt gttattcaga gaggaactac tcaatgtcga
      781 aatgcgccgt ctgtggaatg atagtctggt tggggtttga gatgttacgg ggatcgtgcg
      841 tcgattctag caggatgtcc tcagggatgc cgatacatcc tttttcgcca cacgaatgtc
      901 acgccctcga gcctcacacg gaaattgcaa tggagaacga aactccacat aaggttctct
      961 caaccgtggg cgggccaagc tcaactgaca ctcctttaac tccgtccacc gggtcgctcg
     1021 ttattaccga ggtatctcct gtcgtcactt gctaactccg gtttacgcgc gaccttgctt
     1081 cacttatcgg gctgcagttt ctgaatccgc tggttgattt ctttcattca gcgacgcttg
     1141 tgtctgaagg cttctgcggc ggggggtacg taccgctcct ggatccaagt ataaacagcc
     1201 cttctcgaac agccgaaata ctcaccgacc tcgtccatcc cgttgccgta atgctgagaa
     1261 gacgggttgc ggcgctaaat gatcggatac cctaagatga tgtatctgat gcacttacaa
     1321 atgatcattt ccgatagcaa ctcgcgaacc cgtagccttt tttcagaatc gagtagagtg
     1381 ggattctgaa aaagtggatg ctcgatcccg ggagagatcg tgtttaaacg ggccttttct
     1441 tcgggatctg tttcagcgct gcgaggccca ttaatcgaac cgtttgttag ctgtcgacgt
     1501 tagctgcaac cggtgaatca tgtttacgca ccgatgaatt aggaatgggc gataccgata
     1561 atcctaaaag cgatgcggat tatacaatgg ccgcagtgat cgagcacacc cgtgcttttg
     1621 tacgaactct cggaataaca aacgttacgt tggcgggcca ttccagcgga cggtcaacgg
     1681 ggtcgggctt tctattctta cgtctcctcc gaagccgttg ccagtaatgt tttgatccgc
     1741 aggccgcaga gggctgcagc gtcaggcttg ccatattcac aatttaccaa ccgataataa
     1801 attcgtttgt tcataccgcg catagcggct taccacgttc cacaagaatg cacggtttcg
     1861 gtgccgaaag gcattggtgt aggagcaatc acttgcgccg tgagcgagat cggaatcagt
     1921 ccgcctctga ttgcttggga cgaaatcttt gcatgaatgg ggaagcctag gcgtgagaac
     1981 tttctgttct ctcggcaatt tgtatgctaa cgagcacatt ggtagaaccg gatcatggaa
     2041 accagatcgt gagatgtagt gttatcaagt cagagcattc tgaatcgtcg taataccgaa
     2101 caatttgact acccaccgga gggagaaatg attagagcgc tgctcattac agcaaacctc
     2161 gtcctgatac tgtccagcgc agcctttgcc caagatgcaa aactgatcga ggccgccaaa
     2221 aaagagggcg gcaaagtcgt ggtctacggc tctctcgatt cggatacggg agatgccatc
     2281 ggcaacgcct ttctcaagaa aaccggaatc aagatggact attggcgagc ctcgtcgacc
     2341 aaggtcatgg accgggctac gagcgaatat cgcgccggca agccgctttt cgacgtgctc
     2401 atcaacaacg ccagtccaat gcagattctt tttaaggaag gaattgtagg caagtacaac
     2461 tctccttcgg ccaaaggttt ttcaaaggat gtgattgatc ctgacttggg acctagatat
     2521 cgcaacgtgg tgattggaat tatctacaat aaagataccg tcaaaccagc ggacgctccg
     2581 aaatctctgg aagatctggt gaagcctcag tacaaaggta aagtagttat ggccgatccg
     2641 cggcaacaca cgacgactac gcagtggctc gccagcctgc ataaagtgat gggacaggaa
     2701 aaagcagaca agtttatccg cgatttggca gcaaccaagc cggcgctagt ggaatcttac
     2761 attccggccg cagagcgggt gataactgca gagacgccca tcgcgatagc tttcgtcagg
     2821 tatgtttttc tttacgggca aaaaggagcg ccgcttgatt acgtgagatt ggggaagatg
     2881 ttaggcgatg gacatcatat tgcgcctagc agtaaagcat cgaatccgaa cgcggcaaag
     2941 gcctttatcg atttctttct gggcgacgag agtatggagc tcatgaccaa aatc
//
LOCUS       DASUTG010000548         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_380924, whole genome shotgun sequence.
ACCESSION   DASUTG010000548 DASUTG010000000
VERSION     DASUTG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380924"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VF089_12615"
     CDS             <1..573
                     /locus_tag="VF089_12615"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6174852.1"
                     /translation="PTLIALGGTVKATSADGSRVIPLDEFFTDYYETVLKPDEIVTEV
                     FIPKMAPNSAGAYLKFLPRTADDYATVSAAAVLTLDKTGKTINDVRIALGSVGVTPIR
                     ATAAEALLRGQPLKAEAFAEAGEKAKEAVDPVSDFRGSAAYKKEMAGVFVRRALEKAL
                     ATLRQQARPRPGLKAIKKAIKKAVKKGKKR"
     gene            570..1022
                     /locus_tag="VF089_12620"
     CDS             570..1022
                     /locus_tag="VF089_12620"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6174853.1"
                     /translation="MKFTQSAQIPVAREPLWEFLMDVPKVAKSLPGVESVNKIDDTTY
                     EGALTVRVGPIKLNLQGKIIVEEQSKENWRAALRAEAKDRMAAGAVNGKTNMVLKELG
                     ANQTELLVETDVNILGKIGEFGQPIIRKKADSMLKEFVENIKKQLTAK"
     gene            complement(1159..1920)
                     /locus_tag="VF089_12625"
     CDS             complement(1159..1920)
                     /locus_tag="VF089_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6174854.1"
                     /translation="MGLNKEKSPRVTYLTIEHPPVNVLGIELLRELEQELTACAADST
                     TDVVILRGAGTRAFSSGVDIRDHTREKVPEMLEIVHGVIRKLLGLPQVTIAAVRGVCF
                     GGGCELATSCDLIVAAEDSSFATPEIHVGCYPPVALARFSTLIGYHRAAEMILTGRRY
                     SAREALDLGLINRVFAKDQFNDGLTLLLDELLSKSGAVMRIALKGLRELSLRDFSGAL
                     SRSEEIYCRELLRTEDVDEGVRAFLEKRMPNWKHR"
     gene            2065..>2609
                     /locus_tag="VF089_12630"
     CDS             2065..>2609
                     /locus_tag="VF089_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174855.1"
                     /translation="MKHPRLIAMTLAAWFALGMRPGFAAPDEYDDSQSHPLRIVAYLL
                     HPAAWLVEWAVFRPFHSLVSANEPMEAIFGHEPHPPVLAEPQPNRDYGMPRRVPVKPA
                     PPPAPVAAAPEPVREVVKVVEVPKETIVYRDVEKVVEVEKFVFPGIAFQFDSAELTDW
                     GKGNAYLAAEKLKAKGDISVI"
BASE COUNT          640 a          707 c          708 g          554 t
ORIGIN      
        1 ccgacgttga ttgccctcgg gggtacggtg aaggcaacat cggccgacgg gagccgggtc
       61 attcccctgg atgagttttt tactgactac tatgaaaccg ttctcaagcc ggatgagatc
      121 gtcaccgagg tgttcattcc caaaatggcg cccaacagcg ccggcgctta cttgaaattt
      181 ctgccccgga ccgcggatga ttatgccacc gtttcggccg ctgcggtgct cacgctggac
      241 aaaactggaa aaacgatcaa tgacgtgcgt attgccctag gcagtgtcgg cgtgactccg
      301 atcagagcca cggcggcgga agccctcttg cgtggccaac ctctgaaagc ggaggctttt
      361 gccgaggccg gagaaaaggc gaaagaggcg gttgatccag tgagcgattt ccgtggctct
      421 gctgcttaca agaaggaaat ggcaggggta ttcgttcgcc gcgcgctgga gaaagctctc
      481 gcaaccttgc gtcagcaagc caggccgagg ccgggtctga aggcaatcaa gaaggctatc
      541 aagaaagccg taaagaaagg gaagaaacgt tgaaattcac tcagagcgcg cagatcccgg
      601 ttgcccgtga accattgtgg gagtttctaa tggacgtgcc gaaagtagcc aagtcgctgc
      661 ccggtgtcga atccgtcaat aaaatcgatg acaccaccta tgaaggagcc ctcaccgtcc
      721 gagtagggcc gatcaaactc aaccttcaag gcaagatcat cgttgaagag caaagcaagg
      781 aaaactggcg cgccgcgctg cgggctgaag ccaaagatag aatggccgca ggcgccgtta
      841 acggaaaaac caacatggta ttaaaggaac tcggcgcgaa tcaaactgag ttgttggtcg
      901 agaccgacgt taacatactc ggcaaaatcg gtgagtttgg ccagccgatc attcgaaaga
      961 aagccgactc gatgctcaag gagtttgttg aaaacatcaa gaagcaactc actgccaagt
     1021 agccgtcggc tttttgatga cggcgccgat ttccccagac ttgaattcct gcctgcgggt
     1081 tacctaggct aaattaccgc cgcaagagcg cggcctcatt gatcttctcg ctggtgaagc
     1141 tgaacgccag catgccaact atcgatgctt ccaattgggc atccgttttt ctaggaatgc
     1201 ccgaacgccc tcatcgacgt cctcagtgcg aagcaattca cggcagtaga tttcttcgga
     1261 tcggctaagc gcaccggaga aatctcgcaa agacaactct cgtaacccct tcagcgcaat
     1321 ccgcattaca gctccgcttt ttgagagaag ttcgtcgagc aacaatgtca gaccgtcatt
     1381 gaattgatct ttcgcaaaga ctcggttgat caatccaaga tcaagcgctt ctcgagcgct
     1441 atagcggcgt cccgttagaa tcatttcggc agcccggtga tacccgatta aggtcgagaa
     1501 acgcgccagc gcaacgggcg gataacagcc cacatgtatt tcgggggtgg caaatgagct
     1561 atcttccgcc gcgacgatca agtcgcacga agttgcaagc tcgcaaccac caccgaaaca
     1621 caccccgcga accgccgcga tggtcacttg tggcagaccc aacagcttgc gaatcacccc
     1681 atgaacgatc tcgagcatct cgggaacctt ttcccgtgtg tgatcgcgaa tatccacccc
     1741 agaagaaaag gcgcgcgtgc ctgcgcctcg taggatgacg acatcggttg tgctatcggc
     1801 agcgcatgct gttagttctt gctccagctc gcgcaacaat tcgataccta gaacgtttac
     1861 cggcggatgc tcaatggtca ggtaggtcac ccgtggactc ttttccttat ttagtcccat
     1921 gtcatcccta taccttcgaa tctggcaaaa ggcgagtcgg tacacgtgta aggaacaggg
     1981 tcgagggatg tctgacaaaa ctggtttgac aaagaaaaca ccgcaaccta gacttacggt
     2041 ttcgagctcc taaggagggc tgccatgaaa catccacgat tgatagccat gactcttgct
     2101 gcctggtttg cgttaggaat gagacccggc tttgctgctc ccgatgaata cgatgatagc
     2161 cagtcccatc ctttgaggat cgtcgcctat ttacttcatc ccgcagcctg gttagtggaa
     2221 tgggcagtct ttcgaccttt tcattctctc gtcagcgcca atgagccgat ggaggcaatc
     2281 tttggtcatg aaccgcatcc gccggtcttg gctgagccac aaccgaaccg agattacggg
     2341 atgccgagac gggtaccggt gaagccggcg ccgcctccag cccctgtagc ggccgcaccg
     2401 gaacccgtac gtgaggtcgt gaaagtcgtc gaggtgccaa aggagacgat cgtttatagg
     2461 gatgttgaga aagtcgttga agtcgagaag ttcgtttttc ctgggattgc ttttcagttc
     2521 gattcggccg agctcaccga ctggggcaag ggcaacgcct atcttgccgc ggaaaaactc
     2581 aaggctaaag gcgatatctc cgtgatcat
//
LOCUS       DASUTG010000549         6030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_678284, whole genome shotgun sequence.
ACCESSION   DASUTG010000549 DASUTG010000000
VERSION     DASUTG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678284"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF089_12635"
     CDS             <1..415
                     /locus_tag="VF089_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6174856.1"
                     /translation="SGIGSLDFATSVIGALVSTAFGFLSSFLGIGGGILYVPALVYLL
                     RVPVHIATATSLFVLMITSFTASVTHFLTELFHAGTGRAVALSIGAVVGAQAGAVLSQ
                     KIRGEWIICSLALALSLCGYSTAEPIVVVTVRFGS"
     gene            complement(495..887)
                     /locus_tag="VF089_12640"
     CDS             complement(495..887)
                     /locus_tag="VF089_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174857.1"
                     /translation="MAQIKHIAIRTRNVDQTAAFYKDAFGLTQVGMGKNGIYLTDGHL
                     NIAILKFQEGEDGEPLRLGIDHVGFQVEDLDSALARIESLRGTALSERTEVRPKDAAE
                     RQSYYEIKCVGPDEQVIDISTVGWVGAK"
     gene            complement(1031..2365)
                     /locus_tag="VF089_12645"
     CDS             complement(1031..2365)
                     /locus_tag="VF089_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174858.1"
                     /translation="MHNLNRSSQLLGVIGVIVSLFFPAGAYSADTPERPLEKLTIAYS
                     SVSGNMAPLWITHERGFFRKNGLDVQIVFIESGSTAVQSLSNKDVAFAQMAGAGVLQS
                     RLRGSDVVMIAGFLNTLDYQLMVEKSITRPEQLRGKSMAVSRFGSSSDFATRYALEKL
                     GLVPEKDVTILEIGSQPARFAALESGKIQAAMIAVPLTVRAKALGFRVLADLQMLGLE
                     YQHTGLVTTQALIKSRPDLVRNVMKSYVEGIHYYKTNRPESIAILAKYLKTNDSELLN
                     EVYTDLGLTLTPQKPYPTLRGIDIMLRELAARDAKAKAARPEDFVDLTFIKELDSSGY
                     IDRLYKTQPVVATRQEPPAAAPPAPAPKTPAQPKAAPPPAKVATAKPAPAAAAPSDGA
                     QEYTVVSGDTLSHLALRFYGDHYQWNKIYEANKSTMKNPNFLFIGQRLFIPS"
     gene            complement(2395..3141)
                     /locus_tag="VF089_12650"
     CDS             complement(2395..3141)
                     /locus_tag="VF089_12650"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6174859.1"
                     /translation="MGAESGVSLKWKIGGVYTVVMFILGLLLIAALYQLARSAMRDQL
                     DKRALAIATNFSDAAASHIVSQNPLALHALAAKYTLFEGVAYVYINDTRGEVIAHTLS
                     SFPQHLREGATGGAQREPRRRTFSLQDKTVYETSVPVLDGQAGTAHVGFWSDAVDQEV
                     QSFIWPMIGVIALVPLVGAALSFVIANWIVRPIIGLTEVADKVTKGDLEMSIGNCVVS
                     RDEIGELARSLERMRSSLRAAMLRLNRETT"
     gene            complement(3182..4450)
                     /locus_tag="VF089_12655"
     CDS             complement(3182..4450)
                     /locus_tag="VF089_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174860.1"
                     /translation="MVEPLSLDENKTLVETHFGFRDMPFGVAPDPSFFFPSQHYAEGL
                     DILAEGIKAKKGLLLVTGEVGTGKTILLRKLMRHLDASTRFVFLSTSHLTSDGLIEFI
                     VEDLGLPTQGKTGFEIAQALKDYLLQQSRAAKTVALLIDEAQKLSDGTLESLCDLSNL
                     ETDQEKLLQIVLVGQPELISKLRKPALQRIKQRVAIHYQTRALSFASDMEHYIRHRLR
                     AAGYQGPDIFNQKALETIWSYSSGTPRVINTLCDNALAAASRAGRKRVSAYVIVKAAA
                     KLMLDRPTDDRTSGASRNGVARATAGDFGAHNSQASQQAAETVRTFINQRSNQVAAPV
                     SPPKDRTVASQFFDFMARAATEGIGPMAKLIIDDQIADLGESRDSFPQNKLAELIERV
                     SREIFNGTMRARFLDKMAQAVSALQTMRTF"
     gene            complement(4638..5882)
                     /locus_tag="VF089_12660"
     CDS             complement(4638..5882)
                     /locus_tag="VF089_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174861.1"
                     /translation="MALRTKLTDQIHQIRQTDSLLWLVALGHTFTHWCPATFYLLLPY
                     LVQELGLTYSQAGFLVTIRAAANLLVNVPAGILVDIIGRKALLMGLALLATGVPYFLV
                     GLSHSFFWVAVFMAFVGVGNYLWHPAAISTISERYPSKRGFAIAIHAIGPNIGESIAP
                     LFVGVLLLTLSWRSVLFVNLIPGIVIALILWKFLFGQLQTRAQAKKTLPLTEYLAGMK
                     KMAQNPSILLLVLVAGMRSMTQQGLHTFLPIYLTHDLGLSSAMAGLYLSITQTAGMVG
                     TPIAGSISDRSGRKRVLTAGLFSTSVVLFVLAYFQMTWLFITGLAILGFFLYAVRPVI
                     WAWVLDLSPKELGGSMVSFFSGSQSLLSSLSPLICGFIADRWGILTAFYFLAGTVLLA
                     NLVVLAIREPQPKAPALVEELA"
BASE COUNT         1357 a         1586 c         1636 g         1451 t
ORIGIN      
        1 gtcaggcatt ggcagtttag atttcgctac ttcagtcatc ggtgcgctgg tgagcacggc
       61 cttcggattt ttatcgagtt tcttggggat cggcggcggc attctctatg tccccgctct
      121 ggtatacctg ttgagagttc cggttcatat cgccacggcc acttcattgt tcgttctcat
      181 gattacatcg tttacggcta gcgtcaccca tttcctcacc gagctttttc atgccggcac
      241 aggtcgtgcg gttgctctgt caatcggcgc ggtggttggg gcgcaggcgg gcgccgtgct
      301 atcgcagaag attcgcggcg agtggatcat ctgcagcctc gcgttagcct tgagcttatg
      361 tgggtattcg actgctgagc caatcgttgt ggtgaccgtc aggttcggtt cttgagcggg
      421 tgacctgatt gcgctctgtg attttgtagc ggaactaacg acgtgaggcg atgccgcctc
      481 gcaaagctcg ctcgctattt cgcccctacc cagccgaccg tggagatgtc gatcacctgt
      541 tcatccggac cgacgcattt gatttcataa taggactgcc gctctgcggc atctttcggg
      601 cgaacttcgg ttcgttcact caaggcagtt ccgcgcaacg attcgatccg ggctaaagcg
      661 ctatcaagat cttcaacttg gaaaccgaca tgatcgatgc ccaatcttaa aggctcgcca
      721 tcttcgccct cctggaattt caggattgcg atattaagat ggccgtcggt taaatagatg
      781 ccattttttc ccatacctac ctgagtaaga ccgaatgcat ctttgtaaaa cgcggcggtt
      841 tgatcgacat tgcgggtccg tattgcaata tgcttgatct gagccatcgc tttcactcct
      901 cgtaccagac catgctgcct atgctcgttt ggtatttagc agaactactt atcgcaatga
      961 tcgaaagtat tgctacatta tcgagcaatc gaaccccatc gtagagtagt cggctcgagc
     1021 tcatgcgggc tcacgaggga ataaacagcc tttgtccgat aaataagaaa ttgggatttt
     1081 tcatggtcga cttatttgcc tcatagattt tgttccactg gtagtgatct ccgtaaaaac
     1141 gcagtgccag gtggctgaga gtgtctccgg ataccacggt gtattcttgc gcaccgtcac
     1201 tgggcgcagc tgcagcaggc gctggcttgg ctgtggcgac tttcgctggc ggcggcgcgg
     1261 ctttcggctg ggccggcgtt ttgggggcgg gagcgggcgg tgctgcggca gggggctcct
     1321 ggcgcgtggc cacaaccggc tgggttttat aaagtcgatc gatatagccg gagctgtcga
     1381 gctccttgat gaacgtcaga tcgacaaaat cctccggccg cgccgctttg gcttttgcat
     1441 ctctcgccgc cagctcgcgc agcataatgt cgatgccccg gagcgttgga taaggtttct
     1501 gcggcgtgag tgtcaggccc aagtcggtat agacttcgtt caaaagttcc gagtcgttgg
     1561 tctttaggta ttttgctaaa atcgcgatcg attcagggcg attggtttta tagtagtgaa
     1621 tcccttcgac gtaagatttc atcacattac ggacaagatc aggccgtgat ttgatcagag
     1681 cctgagtcgt caccaagccg gtatgttgat actccaatcc gagcatttgc aggtcggcca
     1741 gaacgcgaaa cccaagtgct ttggctctga cggtcaacgg cacagcgatc atcgccgcct
     1801 ggattttacc ggattcaagc gctgcgaatc gtgctggctg actgccaatc tccagtatcg
     1861 tgacatcctt ttccggcacc aggcccaatt tctcaagtgc atagcgggtg gcgaaatccg
     1921 aagaagaacc gaaccggctt accgccatag atttccctct gagttgttcg ggtctcgtga
     1981 tgcttttttc aaccatcaat tgataatcca aagtgttcag gaaaccggcg atcatgacca
     2041 cgtcggatcc cctgaggcgg ctctgtagca caccagctcc ggccatctga gcgaatgcca
     2101 cgtctttatt gctcaagctt tgtactgcgg tgctgccgct ttcaatgaag acgatttgca
     2161 cgtcgagccc gttcttgcga aagaagccgc gctcatgagt tatccataaa ggcgccatgt
     2221 tgcctgaaac agagctgtac gcgatggtaa gcttttcgag cggtcgctcc ggcgtgtcag
     2281 cgctgtaagc gccggcaggg aagaagagcg aaacgataac accgatcacc cctagtaatt
     2341 gactagatct gttcaagtta tgcataaccg ttctccctct ggagtttgac aaaatcatgt
     2401 ggtttctcga ttcaagcgca gcatcgcggc tctgagactc gaacgcatgc gttcaagaga
     2461 acgcgccaat tcaccgattt catcgcgcga gacgacacag ttaccgatcg acatttcaag
     2521 atcacctttc gtcaccttat cggccacttc agttagaccg atgatgggtc gaacaatcca
     2581 gttggcgatg acaaaagaga gtgccgcacc gaccagcgga acgagcgcaa tgacgccgat
     2641 cataggccag ataaagcttt gcacttcctg gtccactgcg tcgctccaga aacccacgtg
     2701 cgcggttccc gcttgtccgt cgagaaccgg gacgctagtc tcatacacag ttttatcctg
     2761 tagcgagaac gtccggcgcc ggggttcacg ttgcgctccg ccagtagctc cttcacggag
     2821 atgctgcgga aaagaactca gcgtgtgagc aatgacctcg ccccgtgtat cattgatgta
     2881 aacgtaagcg actccttcga aaagcgtgta tttggccgcc agcgcgtgga gcgcgagggg
     2941 attctgactg acgatatgac tggcagcggc atcgctgaag ttggtcgcga tggcgagggc
     3001 gcgcttgtcg agttgatctc gcatcgccga tctcgctaac tgatagagtg ccgctatcaa
     3061 caggagtccc aatataaaca tgacgacggt atagactccg ccgattttcc atttcaaact
     3121 aacacccgat tctgcaccca taattcatct tctcctttaa gtaggaaccg ataccgccgg
     3181 gctaaaaagt tcgcatcgtt tgaagcgcgc tgaccgcctg cgccatctta tccaggaaac
     3241 gagctctcat cgtgccgttg aaaatctctc ggctcactcg ctcaatgagc tcagcgagct
     3301 tgttttgcgg aaagctatcg cgagactcgc cgagatcggc aatctgatca tcgatgatca
     3361 gctttgccat cggtcctatg ccctcggtag ctgctcgagc catgaagtcg aaaaattgcg
     3421 atgctacggt gcggtccttg ggagggctca ccggcgctgc cacctggttt gatcgttgat
     3481 tgatgaaggt gcggactgtt tcagcagctt gctggcttgc ttgagaattg tgcgcgccga
     3541 aatctccggc cgtagctcgg gccacgccat tacgggatgc gccgctggtt cgatcatccg
     3601 tcggccgatc caacattagc tttgccgccg ctttgacaat gacgtaagcg gaaactcttt
     3661 ttcggcctgc tcttgaagcc gccgccagcg cgttgtcgca tagagtgttg atcacccgcg
     3721 gcgtccctga ggaatacgac cagatcgtct ccagcgcttt ctggttgaag atatccgggc
     3781 cctggtagcc tgccgcccgg agacgatggc gaatgtaatg ctccatgtca ctggcgaacg
     3841 ataatgcgcg ggtttggtaa tgaattgcaa ctctttgttt aattctttgc agcgccggtt
     3901 tgcgcaattt gcttatcagt tcaggttgtc ccaccagcac gatttgaagc agtttctcct
     3961 gatctgtttc tagatttgaa agatcgcaca ggctttccag tgttccatca cttagctttt
     4021 gcgcttcatc gattagtaga gccacggttt ttgctgctct actctgctgc agtaaataat
     4081 ccttgagcgc ctgcgcgatc tcgaatcccg tcttgccttg agtggggaga cctagatctt
     4141 cgacgataaa ttcgatcaag ccatcgctgg taagatggct ggtcgaaagg aagacaaacc
     4201 gcgtgctcgc gtcaaggtgg cgcatgagtt tgcgcaacag gatggtcttg ccggttccaa
     4261 cttcaccggt tacgagcaat agaccctttt tcgctttgat gccttcggcg agaatgtcta
     4321 agccttcggc ataatgttga ctgggaaaaa agaagctggg gtctggagcg acgccaaaag
     4381 gcatgtcgcg aaaaccgaag tgagtttcta ccaacgtttt gttctcgtcc aagctaagag
     4441 gttcgaccat tattctcccg tctgctcaac ctgccatgaa tcagatgaaa tcaaaccggc
     4501 cggtgctcga aaaccggtac ccgtgattaa ctttgatagt cgtacgagct gaagcttgtg
     4561 atcggtggcg cgagcagcaa ttcatagtcg attttcgatt tccttgtcaa gaacaagcgc
     4621 ctcagcgctt ctccggttca ggcaagctct tcaaccaggg ccggcgcttt gggctgcggt
     4681 tctcttatag caagaacgac aaggttcgct aagaggacgg ttcctgcgag aaagtagaac
     4741 gcggtgagaa tgccccagcg gtcggcgata aagccgcaga tgagcgggga cagcgagctt
     4801 agcaacgact gtgaaccgga aaagaaactc accatgctcc cgcccagctc cttggggctg
     4861 agatcgagta cccaggccca gatgacaggg cgcaccgcat ataaaaagaa accaagaatc
     4921 gccagaccgg tgatgaatag ccaggtcatt tgaaagtaag ccagaacgaa aagcacgacg
     4981 ctggttgaaa acaacccggc ggtgaggact cgttttctgc cacttcgatc cgagatactc
     5041 ccggcgatag gcgttcctac catgccggca gtttgggtga tcgaaagata tagtcccgcc
     5101 atggcactcg acaagccgag gtcgtgcgta agatagatgg gcaagaaagt atgtaatccc
     5161 tgttgcgtca tggaacgcat cccggcaacg agcaccagca gcaggatgct cgggttttgc
     5221 gccatttttt tcatgccggc caaatactct gttaacggca gagttttctt cgcttgggcg
     5281 cgggtctgaa gctggccgaa gagaaatttc caaagaataa gagcgatgac aatgcctgga
     5341 atgagattga cgaacagaac cgagcgccag gacaaggtca ggagcagcac gccgacgaat
     5401 aacggcgcga tactttcacc gatgttaggg ccgatggcgt ggatcgcgat ggcaaagccc
     5461 cgtttgctcg gatacctttc ggaaattgtg gatatcgccg ccgggtgcca gagatagttg
     5521 cccacgccga caaaggccat gaacaccgct acccagaaaa agctgtgact taagccgact
     5581 aagaaatagg gaacgccggt ggcaagcagc gccaatccca tcagcaaagc ttttcgaccg
     5641 atgatatcga cgagtatgcc tgctggaaca ttaacgagca ggttggccgc tgcgcggatg
     5701 gtgacaagaa aacctgcttg gctataagtc aatcccagct cttgaacgag ataggggagg
     5761 agcagataaa aggtcgctgg acaccagtgg gtgaaggtgt gacctaaggc gaccagccag
     5821 agcagcgaat cggtctgtct tatttgatgt atctggtcag tgagtttggt ccgtagcgcc
     5881 atcgtcgtcc gtcaatcgcc cagcagcaac cctacttctt agaccgccat cgcgatattt
     5941 acaaacaatt ctcaagaggc cctggatcga gtctgtgaaa attaagcaat cctgttgctt
     6001 ctagggccgg gtccaggttg cgtgaagcgc
//
LOCUS       DASUTG010000550         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_529299, whole genome shotgun sequence.
ACCESSION   DASUTG010000550 DASUTG010000000
VERSION     DASUTG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529299"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1466
                     /locus_tag="VF089_12665"
     CDS             210..1466
                     /locus_tag="VF089_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6174862.1"
                     /translation="MRSFLRLLKPLRIRSSQFFHLLFLKYYENMLGLRAASLTYATLL
                     SLVPLLAVTFSVLKAFGVQQLIEPFLTQALAPLGPDRVEITRRLIGFVDNTRVDVLGA
                     VGVAGLFYSVIYLVSTVEESLNQIWRTQPSRTWTRRYGEYMGLLLVGPVLIFAAFAVI
                     ASAQSYWVVQRLVDTTVFGLRFALLTRFLPFFFLWAAFAFLYKVMPSTHVPLSSSIIG
                     AAVAAILWQLLSIVFTAFIVNSVSYAAIYSGFAILVVFLVWLYAAWLVILIGAQVGYL
                     HQHSSVYLPGAAGKQGHLFEEQVALSLMREIGQRFITGKPPATEKNLSRALGVPPDQL
                     DSLLEKYIGRGLVLRSAEPAGISLARPPEQITATEILDVLHGAADSSGDNNDPVAEFL
                     RRREAVVHETLAAVTLKDLANPDRSV"
     gene            1548..1697
                     /locus_tag="VF089_12670"
     CDS             1548..1697
                     /locus_tag="VF089_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX6174863.1"
                     /translation="MLWTIFVVLLVLWALGFVSGYAIGGFIHLLLVIAVVVLLIQLIQ
                     GRSSI"
     gene            2046..2603
                     /locus_tag="VF089_12675"
     CDS             2046..2603
                     /locus_tag="VF089_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX6174864.1"
                     /translation="MKKVFLSLVSWALISSLAGVATVAAAPRQGASEVQGSVGFFHAE
                     DSDFGNLNLDVSYGYYLSPGWQLGFRQALNYLFVDDASDSWIATTAPFINYHFRVTDI
                     VVPYLGAFIGAAWNDDDVTGTLGPQLGVKLFVHEQTFVNLGYRYEWFWDKFERINNES
                     DNGNHIFNIGIGFTWGGSGAGTRRP"
     gene            3016..3240
                     /locus_tag="VF089_12680"
     CDS             3016..3240
                     /locus_tag="VF089_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174865.1"
                     /translation="MKNYMLLLALLALTQSSCAFVGGAATGAVATGAGYEIQAKRQMD
                     RLDEDFKNRRISREEYEDRKKQIEKGSIIY"
     gene            3253..>3692
                     /locus_tag="VF089_12685"
     CDS             3253..>3692
                     /locus_tag="VF089_12685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX6174866.1"
                     /translation="MVLKSIKQFHFISAALLATLLFGCVIADGAAPRADRRNDDRGPP
                     AEARQLDPRQAERFYRIMTPLLKSMNDPKSPRDVKIGIIDEPEINAANAGGGQFLVTT
                     GLLEKASDEQLRGIMAHEIAHDDLGHVAKAQGLGVGLNLGVILL"
BASE COUNT          795 a          938 c          997 g          962 t
ORIGIN      
        1 ccgacgggca agaagcagtt gcggaagcgc taaatcaggt ttgggacatt catctcgttt
       61 gatccaattg tcatggtgaa agagctcgcc tcagccactt aagtgacttc cggcatattt
      121 tttgccgctt ccccgtagtc atgtgctagt tgaggctcgg ctgggcagcg ggtggattgc
      181 ctaattttgg acccgggaga atcttgaaag tgcgttcgtt tttgcggctg cttaaacccc
      241 tccggatacg ttcctcccaa tttttccacc tgctctttct aaagtactac gaaaacatgc
      301 tgggcttgcg ggcggcaagc ttaacgtatg cgaccttgct gtcgctggtg ccgcttcttg
      361 cggtcacatt ttccgtcctc aaagctttcg gcgtccagca gttgatcgaa ccatttttga
      421 ctcaagctct ggctccgctt ggccccgatc gggttgaaat cactcgccgt ttgattggat
      481 tcgtcgacaa tacccgggtc gatgttctgg gcgcggttgg tgtcgcaggg ttattttact
      541 cagtgatcta tctcgtaagc accgttgagg aatcgttgaa ccagatctgg cgaacccagc
      601 cctcgcgcac ctggacccgg cgctacggtg agtacatggg attgctgttg gtcggtccgg
      661 ttctcatctt cgccgccttt gctgtcattg cgtcggcgca gagttactgg gttgtgcaac
      721 gactagtcga tactactgtg tttggcctta ggttcgcgct gctaacgcgc tttctgccgt
      781 tcttctttct ttgggcggcg ttcgcctttc tctacaaggt tatgcccagc acgcacgtgc
      841 ctttgagttc atcgatcatc ggtgccgcag tggcggcaat tctgtggcag ttgctgagca
      901 tcgttttcac cgcgttcatc gttaactcgg tgagctatgc agcgatctat tcgggcttcg
      961 cgattttagt cgtctttctt gtatggttgt atgcggcctg gctggtaata ctgatcggcg
     1021 cccaggtcgg ctacctgcat caacactcct cggtttacct gccgggcgcg gctggtaagc
     1081 aaggtcacct cttcgaagaa caagtagcct tatcgctcat gcgtgagatt ggccagcgat
     1141 ttattaccgg caagccgccg gcaaccgaaa aaaatctaag ccgcgcactt ggagtgccgc
     1201 ccgaccagct cgacagcttg ctggagaaat acatcggccg cggactggtc ctacgatccg
     1261 ccgagcccgc cgggatcagt ctagccagac cgccagaaca aataaccgca acggaaattc
     1321 ttgacgttct gcatggggcg gctgactcca gcggcgataa caacgatccg gtagcagaat
     1381 ttctgcgccg gcgcgaggcc gtcgttcacg aaaccctggc agcggtcact cttaaagatt
     1441 tagcgaatcc agatcgctcg gtataactgt tctagtcagt tggtgattgg catgtcctgt
     1501 gcgatgttca tgcaacgaca actgagacta caaccaagga ggcgaccatg ctttggacta
     1561 tattcgtagt gcttctcgtt ttgtgggctt taggttttgt cagcggctac gctattggcg
     1621 gattcattca cctgctttta gtcatcgccg tcgtcgtatt actgatccaa ttgattcaag
     1681 gacgaagttc tatctgagcc gttcgcccgg cgtagggatt actcgtcatc ctaccgttgg
     1741 cctcacgtgc ccagtaggtt agaagatcgg aagcgaattc caagaccaca ggcgtagcga
     1801 aacgagctgc tcttcttcgg cggcagcgcg gttttaaaat acggactagg aaagtctctg
     1861 cgggtgtgag ctggtggggt gtagcgttgt cgctacaagc gaaggctcga ccagtagcgt
     1921 ttgccggaca gggccgacga atctatgcag gaattattct ttaaatacga ggcgcttgtg
     1981 ttttcgggca gttggcatct tcattgctca caaaagcttc aaattttatc agtggaggta
     2041 tttctatgaa gaaggttttc ctgtctctag tgagttgggc gttgatcagt tcgctcgctg
     2101 gagttgcgac tgtcgctgcc gcacctcgac agggcgcgag tgaggttcag ggatcggttg
     2161 gattttttca tgctgaagat tctgacttcg gcaatctaaa ccttgatgtg agctatgggt
     2221 attacctcag cccaggatgg caattaggct ttcgacaggc gctcaattat ctattcgtag
     2281 acgatgccag tgatagctgg atcgcgacaa ccgcaccttt tatcaattac cattttcgag
     2341 tgaccgacat cgtcgttccc tatctcggcg ctttcatcgg cgcagcgtgg aacgatgatg
     2401 acgtgactgg aaccctcggc cctcagctcg gtgtcaaact tttcgttcat gagcagactt
     2461 tcgtgaatct cggttatcgc tacgagtggt tttgggataa gttcgagcgc atcaataatg
     2521 aatccgacaa tggcaaccat atattcaata tcggcattgg ttttacgtgg ggcggttccg
     2581 gcgccggtac gaggagacca taaccgtttt cttgacattt aaataaaccg cgttaaatgt
     2641 aaaaggcttc tcaccctcgc ccggggagaa gccttttgcg ttgtacacga ggaactcgct
     2701 aactctactg tcacagtaga agtcaatccc gctgcgcgga accttcctcg gcaagtttca
     2761 aagctaagta gggcctcaga attcgtatcg gtttgattca gaattgcgcc ttatcagcac
     2821 tgatatttag aaccgtccca caactgcgct aattggcttt ccatttgttc tactttcaag
     2881 acgagcggca atcggccgtt tagttttcac tcttccgcac gaagcttaat tggactttcg
     2941 agtagaattc ccagccgtgg cacatccatt gctctttgta ccttttgaca tcgggtgcaa
     3001 acgaaaggaa gactaatgaa aaattacatg ctcttgctgg cgctgttggc gttgacacaa
     3061 tcgagctgcg catttgtcgg tggagcagcg accggagcag tcgccacggg cgcaggttac
     3121 gagatacagg cgaaacggca aatggaccgt ttggacgaag atttcaaaaa tagaagaata
     3181 tcccgcgaag agtatgaaga tcgcaagaag caaatagaaa aaggatcgat catttactag
     3241 agcgatcctc ccatggtgtt gaaatccatc aagcagttcc actttatttc ggcggcgctg
     3301 ttagcgacct tgctcttcgg ttgcgtcatc gcggatggcg cggcgccgcg ggccgatagg
     3361 aggaacgatg atcgcggtcc gccggcggag gcgcgccagc ttgatcctcg gcaagctgag
     3421 cgattctacc ggatcatgac gccgctgctg aaaagcatga acgatccgaa aagtcctaga
     3481 gatgtaaaga tcggcataat cgatgaacct gagatcaatg cggccaacgc cgggggcggc
     3541 caattcctcg tgaccaccgg tcttctagag aaggccagcg acgagcagtt gcgtggcatt
     3601 atggcccatg aaatcgctca cgatgactta ggccatgtcg ccaaggccca gggtcttggc
     3661 gtcggcttga atttaggagt gatacttctc ga
//
LOCUS       DASUTG010000551         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_975592, whole genome shotgun sequence.
ACCESSION   DASUTG010000551 DASUTG010000000
VERSION     DASUTG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975592"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF089_12690"
     CDS             complement(<1..231)
                     /locus_tag="VF089_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174867.1"
                     /translation="MTTSFPAHRTVIYRITALFLLINFILHAQGRGLEPGSPAGSSES
                     SANQQSQSLPVYDASPSSGVPGRAYHVNIISRT"
     gene            complement(358..1041)
                     /locus_tag="VF089_12695"
     CDS             complement(358..1041)
                     /locus_tag="VF089_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174868.1"
                     /translation="MRSSILVIHICGGVVGLLSGIAAVAFRKGSPRHRLAGNIFFASM
                     LTMAATAAYLGNFFGGAFTFYLVTTGWLTGRKREGETTLFDWGAFLFALAIGLIIVGH
                     SVRVISGAVAPKPGVPVGMPLFLGCLVLLAAAGDLRMLMRGGVFGRQRIARHLWRMFV
                     GLFIATGSFLAQRRVVAFLGGPEVFLLFAPLPLILMIYWLVRVRSKNVYQAKPGKPDA
                     SPAAAFRFP"
     gene            complement(1054..1458)
                     /locus_tag="VF089_12700"
     CDS             complement(1054..1458)
                     /locus_tag="VF089_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174869.1"
                     /translation="MQHQVKLTITSLLSILFFTFHVSDDIVRGFEPGGFKHISTILIL
                     VVWLYGTLALSGRRSGYIIILVASLLSSLMPLAHMRGRGLAGGSIATSSGALFFVWTL
                     VSIGVTAAVSVILSVRGLWSLPWRRTRKSTNN"
     gene            complement(1596..>2190)
                     /locus_tag="VF089_12705"
     CDS             complement(1596..>2190)
                     /locus_tag="VF089_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6174870.1"
                     /translation="VARDGKEGLEKASTEKPDIILLDVMLPLMSGIDVCRTLRTRGID
                     TPILMLTARSQEMDKVVGLEVGADDYVTKPFSIKELLARIRAHLRRAAKQVAEIESFS
                     FGDVELNFKKYVARKGGQVLDLSAREFEILHYLIRRRGEIVTRDQLLDEVWGYDSTPV
                     TRTVDNHIARLRQKIEPDPSDPQHIITVHRIGYRFIE"
BASE COUNT          527 a          602 c          612 g          449 t
ORIGIN      
        1 agtacggctg atgatgttca cgtggtaggc tcggccgggt actccgcttg atggactggc
       61 gtcgtaaacc ggaagcgact gactttgttg gttggcgctg gattcgctgg agcccgccgg
      121 cgatcccggt tcgagaccac ggccttgagc gtgaagtata aagttgatca gcaggaatag
      181 cgccgtgatc ctgtaaatca cagtgcgatg cgcgggaaaa ctggtcgtca tagtctgttt
      241 cgcctcggtg gtgagagtaa aaaaagcgtg tacttgtctc ttatccgctt atgacgaaac
      301 gctcaaattc atttcgcggt gcccgcttgg ttcaacaata gttggagtct tggaacctta
      361 tggaaatctg aaagccgcag ccggcgaagc atcgggtttg cctggcttcg cctgatacac
      421 attcttcgaa cgaacgcgga ccagccagta gatcatcaag atcaacggta gcggagcgaa
      481 gagtaaaaat acttctggtc caccgaggaa tgcgactacg cgtcgctggg ccaggaacga
      541 tccggtggcg atgaataggc caacgaacat gcgccaaaga tgtcgtgcga ttcgctgcct
      601 gccgaaaacc ccgccacgca tcagcatgcg aagatcaccc gcggcagcaa gtaggaccaa
      661 acaaccaaga aacaacggca tcccgaccgg aactccgggc ttgggagcaa ccgctccact
      721 aatcactctc acgctatggc cgacgatgat cagaccaatc gccagggcaa acagaaaagc
      781 accccagtca aagagggtcg tttcgccttc gcgtttcctg ccggtcagcc atcccgtggt
      841 caccagataa aacgtaaaag cgccgccgaa aaaattgccc aggtacgccg cagttgcggc
      901 catcgtcagc atggaagcga aaaagatgtt tccagccagg cgatgccgag gcgagccttt
      961 gcggaacgca actgccgcga tgccggacag taaacctacg acaccgccgc aaatgtgaat
     1021 aactaatatc gacgatcgca ttggtccacc tccttagttg ttggtggact tccgggtccg
     1081 gcgccagggc aggctccaga gcccgcgcac cgagagaatg acggagacag ccgcggtcac
     1141 gccgatcgaa accagcgtcc agacaaagaa gagcgctccg ctggacgtcg cgatgctccc
     1201 gccggcaagg cctcgccctc gcatgtgggc cagaggcatg agcgaagaca ggagcgacgc
     1261 gaccaggatg atgatgtaac ccgagcgacg tccggagagc gccagcgtgc cgtacagcca
     1321 gacgacgagg atcagaatgg tgctgatgtg cttgaaccct cccggttcaa atccgcgcac
     1381 aatgtcgtcc gacacgtgaa aggtaaagaa gaggatcgag agtagagacg tgatggttaa
     1441 cttgacctga tgttgcaact gttattctcc ctgtagagat tgttgagtct gagcacaact
     1501 tttctatcca tgtgggagcc tagagtcttt gcacttcaaa gtctccacac gctgtaacta
     1561 gtttgtaaaa agttgtaccg gcagggagcg gaggtttact cgatgaagcg atagccgatc
     1621 cggtgaacgg taatgatgtg ctgagggtcg gacgggtctg gttcgatctt ttgccgcagc
     1681 cgggcgatat ggttgtcaac cgtacgcgta accggggtgc tgtcatatcc ccaaacttcg
     1741 tcgagcagtt gatcgcgggt gacgatttca ccgcgacgcc ggatcaggta gtgcaggatc
     1801 tcaaactcac gcgctgaaag atctaaaact tgaccgcctt tgcgcgccac gtattttttg
     1861 aagttcaact ctacatcgcc aaagctaaaa ctctcgattt cggcgacctg ttttgccgcc
     1921 cttcgcaaat gcgcccggat gcgcgcgagc agttccttta tgctgaatgg tttggtgacg
     1981 taatcatctg cgccaacttc gagaccaacc accttgtcca tttcctgact ccgcgcggtc
     2041 agcataagaa tcggagtgtc gatgccacga gtgcgtagcg tgcgacacac atctatgccg
     2101 ctcatcaaag gcagcatcac gtcgagcagg atgatgtcgg gcttctcagt cgatgctttc
     2161 tccaaacctt ctttcccatc gcgggccacc
//
LOCUS       DASUTG010000552         5771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1716094, whole genome shotgun sequence.
ACCESSION   DASUTG010000552 DASUTG010000000
VERSION     DASUTG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716094"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /gene="clpB"
                     /locus_tag="VF089_12710"
     CDS             <1..2355
                     /gene="clpB"
                     /locus_tag="VF089_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX6174871.1"
                     /translation="AESEAEALKDEYVSTEHLLLAMIQDSGEVGKILKDLGATRENIL
                     NGLVSIRGGQRITDQNPEEKYQALEKYSRDLTDLARKGKLDPVIGRDDEIRRVVQVLS
                     RRTKNNPVLIGEPGVGKTAIVEGLALRIVNGDVPEGLKDKRLVALDLGALIAGAKYRG
                     EFEDRLKALLKEVTDAAGRIILFIDELHTLVGAGAAEGAMDASNMLKPALARGELRCV
                     GATTLDEYRKRIEKDPALERRFQPVFVGEPSLEHTIAILRGLKERYEVHHGVRITDGA
                     IIAAATLSHRYISDRFLPDKAIDLIDESASRLRIEIDSMPIEIDEVERKILQLEIEKQ
                     ALKREEDKASKERLTQLEREIQNLKETSNGLKAHWKNEKESIQRIRSLKERLEATKVE
                     EQKAQREGDLNRAAELRYGTLTQLQKELEQANLQLAELQKDQKMLKEEVDAEDVAEVV
                     AKWTGIPVSKMLEGDIQKLLKMEDRLKARVVGQPDAIHAVANAVRRARAGLQDENRPI
                     GSFIFLGPTGVGKTELCRALAEFLFDDEQAMVRLDMSEFMEKHSVSRLIGAPPGYVGY
                     EEGGYLTEAVRRRPYSVVLFDEIEKAHPEVFNALLQILEDGRMTDGQGRTVDFKNTVI
                     IMTSNLGSQFIQDLADGNRKEMEARVTAALRDAFKPEFLNRIDEIIIFNSLGREEIKS
                     IVEIQLKRLRQNLANRKMALEITDDAKELLAEKGYDPVYGARPLKRTIQRLIQDPLAV
                     KILEGEFQEGDRVRIDTDGDALAFEHGGSAATEETLAETGGTLH"
     gene            2724..4160
                     /locus_tag="VF089_12715"
     CDS             2724..4160
                     /locus_tag="VF089_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174872.1"
                     /translation="MADSNSPNQTRRDPRLVLLLVCTGLFMVAINARAMIVSLPTLTE
                     VFQTNLSLIQWALLVYDLAVIGLVLTLSRLGDLFGRKWMYIIGLLLFTFGSTLCGLVQ
                     SPAQLIACRIVQGIGGAMIMAHGRAIASIVVPVSQRGKALGLTSMAYHVGYMTGPTLG
                     GFIIDTIGWRWIFFVNIPTGMVAAYFAWKILADRAPSEERVKVDYLGAALLLITNTSF
                     VYAMNQFPHLGFSHPLVISFAILSAAALFFFIRTELRVKTPILDLNLFRNRLFSAANL
                     SLLFITGTQSAIAVLMPFYLQNLMGFTPTQMGWIIIANSVVIIMVAPIAGGLSDRFGS
                     RLLCSIGATLIVIGQFLIASWDQQSTIFEMIFPQALTGLGWAIFNSPNQSAILGTVPR
                     DKVGAASGMTVTTARIGAVIGIALSGLLLTNGLYGGGMPAGQIESPQSWGASPEIFLD
                     AFSHAIHVINSFAILAIVFSAIRGGNKD"
     gene            4305..5738
                     /locus_tag="VF089_12720"
     CDS             4305..5738
                     /locus_tag="VF089_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174873.1"
                     /translation="MMQPDDAKKTRWTLRLILLNVCIGQTVVGLDQRAITVALPTLTD
                     TFHSAFTTIQWVVLVYDLILIGLIITMGRLGDLFGRRRLYSIGFLIFVAGSALCGISQ
                     TAGQMIFFRAIQAIGGSMITANGRAIVSVALPREERGRALGLTSTAFHVGFLTGPSLG
                     GFLIDTIGWRWIFYINLPIALWGAYLAWKIIPETKAEGKVSVDIPGAILLLLTNGLFI
                     YAIDLLPRLGWRHPTFALILLLSLVAMTLLLFVESKATTPILILSMFRSRLFSAGILS
                     LFLITLSLSAINFLLPFYLQNLLGYSPSQMGWIIIADSLIIMFMAPIAGTWSDRLGSR
                     LLCTIGCVVIVIAQFFVGSLDLESSLPRIMLPLALWGVGWALFNAPNQSATFGAVSHD
                     KLGAAAGMIATTARTGSAIGVAIAATLFSTMLSAAGLSPEQTQSPQSWSAMPESVIGS
                     FNYTIHVINGLTLLAVFFSAVRGNRRD"
BASE COUNT         1240 a         1602 c         1590 g         1339 t
ORIGIN      
        1 gctgagagcg aagccgaggc gctgaaagac gagtatgttt cgaccgagca tttattgctg
       61 gcgatgattc aagattccgg tgaagtcgga aaaatcctta aagacctcgg cgccacgcgt
      121 gagaatattc taaatggcct ggtcagtatt cgcggcgggc agagaattac cgatcaaaat
      181 ccggaggaaa aataccaggc tttggagaag tacagccgcg accttaccga cctcgctcgc
      241 aaaggcaaat tggatccggt tatcggccgg gacgatgaaa ttcgccgggt cgttcaagtg
      301 ctttcacggc gcacgaaaaa caatcctgtg ttgatcggcg agcccggggt cggcaagact
      361 gccattgtcg aaggcctcgc cttgcgcatt gtcaacggcg atgtccccga aggactcaaa
      421 gacaagcgcc tggtcgcgct ggacctaggc gcgctgatcg ctggagcgaa gtaccgcggc
      481 gaattcgagg accgtctcaa agcgcttctc aaagaagtga ccgacgctgc cggtcgaatc
      541 attctgttca tcgacgagct tcacacttta gtgggcgcgg gcgcggctga aggcgccatg
      601 gacgcgtcga acatgcttaa acccgccctg gcacgcggcg aactccgctg cgtcggcgcg
      661 acaaccttgg atgagtaccg caagcggatc gagaaggatc ccgctttgga gcggcgtttc
      721 cagccggtgt tcgtcggcga gccttccctt gaacatacca ttgcgattct gcgcgggctc
      781 aaggagcggt acgaagtgca ccatggcgtg cgcatcaccg atggcgcaat catcgctgcc
      841 gcaacgttat cgcaccgata catcagcgac cgctttttgc ctgacaaagc gatcgatttg
      901 atcgacgaat cggcctctcg actacgcatc gaaattgaca gcatgcccat tgaaatcgat
      961 gaagtcgagc gcaagattct ccagcttgag atcgaaaagc aggcgctaaa acgcgaagaa
     1021 gacaaggctt cgaaagagcg gctgacgcag cttgagcggg agatccaaaa tcttaaggaa
     1081 acttccaacg gcctcaaagc gcattggaaa aacgagaagg aatcgatcca acggatccga
     1141 tcgctcaagg aaaggctcga agcgaccaag gtcgaggagc agaaagcgca gcgcgaaggt
     1201 gatttgaatc gcgccgccga acttcgctac ggcacgctca cccagcttca gaaagaattg
     1261 gaacaagcca atctgcaact ggctgagctg caaaaagatc agaagatgct caaggaggaa
     1321 gtcgacgccg aagacgttgc cgaggttgtc gccaagtgga ccggtatccc ggtttcaaag
     1381 atgctcgagg gcgatatcca aaaacttctg aagatggaag atcgcctcaa ggccagagta
     1441 gttggtcagc ccgatgcaat tcatgcggta gctaatgcag tgcgccgtgc tcgcgccggt
     1501 ctgcaggacg agaaccggcc gatcggttcg ttcatcttct tgggacctac cggcgtcggc
     1561 aaaaccgagc tgtgccgggc tttggctgaa ttcttgttcg atgacgaaca ggcgatggtt
     1621 cgtttggata tgtccgagtt catggagaag cactcggtct cacgtctgat cggcgctcct
     1681 ccgggctacg tcgggtatga agagggcgga tatctcaccg aggcggtacg ccgccggccc
     1741 tactcggtcg tgctcttcga tgagatcgaa aaggcgcatc ctgaagtctt caatgctttg
     1801 ctgcagattc tcgaggacgg ccgtatgacc gacgggcaag gaagaacggt tgatttcaag
     1861 aacaccgtga tcatcatgac gtccaacttg ggcagccagt ttatccaaga tctggcggat
     1921 ggaaaccgga aggaaatgga agcgcgagtc accgcggcct tgcgtgatgc gttcaaaccg
     1981 gaatttctca accgcatcga cgagatcatt atcttcaaca gcctggggcg cgaggagatc
     2041 aagtcgatcg tcgagatcca gctcaaacga ttgcgtcaaa acttggccaa ccggaagatg
     2101 gcgctggaga ttacggatga tgcgaaggaa ctgctcgctg aaaagggata cgatccagtc
     2161 tatggcgcgc ggccgctgaa acggacgatt cagcgtttga ttcaagatcc cttggcggtc
     2221 aagattttgg aaggagaatt tcaggaaggc gacagggtca ggatcgatac tgacggagat
     2281 gccctggcct tcgaacatgg cggctcggcc gcgacggagg agactctggc ggagactggg
     2341 gggacactgc attgaagagc gccacgaaaa aagcggcaaa gaagatccct ccggggctag
     2401 cacgggttcg caaagagatt cctcctccaa ccaaagtttt tcggagcaag aagagcgatc
     2461 agcggcaaca tcgcaaagcg gaactccggc aggaattgaa agcaaaccca ccgggtagat
     2521 cttcttaacg cattcgtcca ttttctcctg aggggcagtc caaccgggct gcctctcttt
     2581 ttgtgtcccg tgctgctgca aagctttgtg cttcgtcgcg ccggtacatt gtttccggat
     2641 actgtgtgcc agtctcagtc atctaattgc gtgccttatt gagtttgagc agcctgtaat
     2701 cagatttcag taattggtaa tgcatggccg attcaaacag cccgaaccaa acccgacgag
     2761 atccacgtct cgtgctactt ttggtctgta ccggactttt tatggtcgcg atcaacgcgc
     2821 gcgccatgat cgtctcgctg cccaccttga ctgaagtttt ccaaacgaat ttatccctca
     2881 tccagtgggc tctcttggta tacgacctgg cggtcatcgg cctggtgctc accctgagcc
     2941 gcctcggaga tctcttcggc cgcaaatgga tgtacatcat cggtttgctg ctctttactt
     3001 tcggctcgac tctgtgcggc ttggtacaaa gtccggcgca attgatcgcc tgccgcatcg
     3061 tccagggaat cggcggcgct atgatcatgg ctcacggccg cgccatcgcc tccatcgttg
     3121 tgccggtttc gcaaagaggc aaagcactgg gattaacctc catggcctat cacgtgggct
     3181 acatgacagg tccaacttta ggcggcttta tcattgatac cattggctgg cgttggatct
     3241 ttttcgtcaa tattccaacc ggcatggtag ccgcctactt cgcctggaag atccttgccg
     3301 accgagcgcc gagcgaagaa cgggtgaaag tcgattatct tggcgcggct ctcctgttga
     3361 tcaccaatac tagttttgtt tatgcgatga atcagtttcc gcatctgggg tttagccatc
     3421 ccctggtgat atccttcgct atcctctcgg ccgcagccct cttcttcttt attcgaactg
     3481 agctgcgggt gaaaactccc attctagatt tgaacctttt ccgcaatcga ctcttcagcg
     3541 cggctaactt aagcctgctt tttattaccg gaacccaatc cgctatcgct gttctgatgc
     3601 ccttctattt acaaaatctg atgggcttca cgccgactca aatgggctgg atcatcatcg
     3661 ctaattcggt tgtcatcatc atggttgcgc ccattgccgg cgggctttcg gatcgctttg
     3721 gttcccggct gctttgcagc atcggcgcaa ccctcatcgt tatcggtcaa ttcttgatcg
     3781 cttcttggga tcagcagtcc acgatatttg agatgatctt tccgcaagcg ttgaccggtt
     3841 tgggttgggc gattttcaac tcgccgaatc aaagcgccat cttgggcacc gtgcctcggg
     3901 ataaggtcgg cgccgcctcc ggcatgaccg tgactacggc gcgtatcggc gcagtgatcg
     3961 gcatcgcgct ctccgggctg ttgctcacca acggtctgta cggcggcggc atgccagccg
     4021 gacagatcga atcgccgcaa agctggggcg catcgcctga aatttttctt gacgcattta
     4081 gtcacgccat tcacgtcatc aatagttttg ccattctggc gatcgtcttc tccgctattc
     4141 gcggcggcaa caaagactga tacattcgtg attctgccga agctcgaccg tgatttgcgt
     4201 tggggtcgga cggggtggag tcgaaaatgg tttcttgccc tcgaccgaag acacctgaga
     4261 gtcacgcggt gcgcctgctg aagccggctt aggtacctga ctgaatgatg caaccggacg
     4321 atgccaagaa aacccgatgg accctgcggc tgatactttt gaatgtttgc atcgggcaaa
     4381 ccgttgtcgg tctcgatcag cgcgcgatca cagtcgcgct gccaaccctg accgacactt
     4441 ttcacagcgc gttcaccaca atccaatggg tagtgttggt ctatgatctg attctcatcg
     4501 gactgatcat caccatgggc cgcttgggtg atctgttcgg ccggcgccgg ctctacagca
     4561 tcggttttct gatatttgtc gcaggttccg cgctctgcgg catctctcaa accgcaggtc
     4621 agatgatctt ctttagagcc attcaggcga ttggcggatc gatgatcacc gccaacggca
     4681 gagccattgt gtcagtcgcg ctcccgcgcg aagaacgggg ccgggctctg ggcctgacat
     4741 cgaccgcctt tcatgtcggt tttcttaccg ggccgtcgct cggcggattt ctcatcgata
     4801 cgatcggctg gcgctggatc ttttatatca acctgcccat cgctctgtgg ggggcctacc
     4861 ttgcctggaa gattatccct gagaccaaag ccgaaggaaa agtatcggtg gacattccgg
     4921 gcgcaatact gctcctcttg accaacggcc tgtttattta cgccatcgat ctgctgccgc
     4981 ggctcggctg gcgccatccg acgttcgcgc tgatattgct gctctcgctg gttgcaatga
     5041 cgcttctcct tttcgtcgaa agcaaggcca caacacctat cctaatccta tcgatgttcc
     5101 gcagccgtct gtttagcgca ggcatcttga gtctcttttt gattacctta agtttgtccg
     5161 ccatcaactt tctcttgccc ttctatttgc aaaacctatt gggctattcg ccctcgcaaa
     5221 tgggctggat catcatcgct gattcgctga tcatcatgtt catggcgccg attgccggca
     5281 cctggtcgga ccggctgggc tcccgcctgc tctgcactat cggttgcgtc gtcatcgtca
     5341 tcgcgcaatt tttcgtcggc tcactcgacc tggaatcatc gttaccccga atcatgcttc
     5401 ccctggcgct ctggggagtc ggctgggcgt tatttaacgc gcccaatcag agcgcgacct
     5461 tcggagcggt gagccacgac aagcttggcg ccgcggcggg catgatcgcc accacggcac
     5521 gcaccggcag tgccatcgga gtcgctatag ccgctactct tttcagcacg atgctatcgg
     5581 cggcaggttt gagcccggaa caaacccagt cgccgcaaag ctggagcgcg atgcccgagt
     5641 cggtcatcgg ctctttcaat tacacgattc atgttatcaa cggactgact ttgctggcgg
     5701 tgttcttctc cgcagttaga ggcaaccgca gggactgatc gattttagtc ggattttttc
     5761 tccctgaaag a
//
LOCUS       DASUTG010000553         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1866242, whole genome shotgun sequence.
ACCESSION   DASUTG010000553 DASUTG010000000
VERSION     DASUTG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866242"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..599
                     /locus_tag="VF089_12725"
     CDS             153..599
                     /locus_tag="VF089_12725"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX6174874.1"
                     /translation="MEPSKSEIRREIEVTRAAMAEKIETLEARVGDVIQEAKRSVDPK
                     YQTQKRPWLMVGLAVATGYLFEGVIFGKAPPRAKGRKFSIDPERRAYLEGKQKAAGGG
                     ILRSIATAAGVALARDYATKIVSGRGLSSRSAANEDRTKVKSPYEL"
     gene            822..1106
                     /locus_tag="VF089_12730"
     CDS             822..1106
                     /locus_tag="VF089_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174875.1"
                     /translation="MKSQRVRWEEQSFWQLVFGQSKVNGAKYDGENLTTSPWRWPTNT
                     YLQRRRQLLKNSQAFDQSGQSEAGVFYDSVYDTGNHAIAEFRDSGSLRNN"
     gene            1332..2891
                     /locus_tag="VF089_12735"
     CDS             1332..2891
                     /locus_tag="VF089_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6174876.1"
                     /translation="MSVKKILQPDKNCWTISEVTESGLLIDGRDYYRAFYQAARSAED
                     YILISGWQFDSDVALLRGSDADYAPASVQFLTFLNDLCEQNQRLHIYILAWDFTVLYS
                     LDREWFQDWYFNWTTNERFTFCFDCCECFGAAHHQKFVVIDGKLAFVGGLDICSGRWD
                     DRDHPVDSPARVNSDQNGYAPFHDIQSYHVGPAAKKLAELFVARWRCVTTGDLELAQS
                     SSNYQIPFRPTVAIPAESIAISRTQSGTVGQQRPIQEIRRLFLDAIDAAEDLIYVENQ
                     YFSSNALYKALVDRMMNSGRSHLEIVLIIAKDAEAFVEQLSIGVVQEKLLRELQSVAA
                     ETGHSFGIYYPASVCGDGRESATYIHSKLFLVDDRFLSVGSANMNNRSLGLDTELNVA
                     WEAPAADSELGRAIRAARIDLLAEHVGAREAQVLENLTRSRGLVKYLNTLADSGSYRL
                     RHHPLDIVSEDYLWVTSVLPEGLPFDPEGPFYQEDRYEGISATEDSFFSRGVSSLKNW
                     LLGLGQTLSPR"
     gene            complement(3002..>4042)
                     /locus_tag="VF089_12740"
     CDS             complement(3002..>4042)
                     /locus_tag="VF089_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX6174877.1"
                     /translation="LALTATAADEVAVDILKQLDLPDAEVISIGIERKNLAFEVIRTV
                     NEDVKQARLKEVLQEQSGGAGIIYVPTVKQVDALCKWLIDEGFRAGQYHGRMKMPARE
                     ANQQAFMANEIDVMVATKAFGLGIDKQDIRFVIHYAVPDSVETYVQETGRAGRDGKPA
                     RAILFYRLEDRRVQSYFLGGKYPRPDESLALYRTLDQMPRREDLGDRTPEQWLAEVTD
                     LAAKRVKVILALLESTGIIQRRPHVSKIRDFNTDQEFATFLEEYEQRHNGDRQRLDAM
                     MLFGQITTCRMRYLLDYFGEEVEADCSRCDNCRAGQEAAESNFRNAALSGEAITPATS
                     SCEIGAAVAANS"
BASE COUNT          910 a         1059 c         1124 g          949 t
ORIGIN      
        1 ccggtaacat cgctggtcgt cggagtcgtc agcggcttat tgctggctcg caaagtacgg
       61 cggtagtaat caacgatcgc agctacaaac tgttagttct tgcttggaac agctagaaac
      121 aacaatgaga gcaaagcgca aaaggatgtg agatggaacc atcgaagagc gaaattaggc
      181 gagaaattga agtaacccgc gccgctatgg cagagaaaat cgagacgctt gaagcacgcg
      241 tgggcgatgt catacaggag gctaaacggt cagttgatcc caaataccag acgcagaagc
      301 gtccgtggct gatggtcggg ctggcagtgg caaccggtta cttgtttgaa ggcgtcatct
      361 tcggcaaagc tccgccgcga gccaaaggtc gcaagttttc catcgatccg gaacggagag
      421 cgtacctaga gggcaaacag aaggcggctg gcggcggcat ccttcggagc atagcgacag
      481 cggcaggcgt ggcgctggcg cgggattatg caaccaagat agtttcagga cgtggtctat
      541 cctcgcggtc cgcagcgaac gaggaccgaa caaaggtaaa gtcgccctac gagctgtaat
      601 cctactgtaa ttctcggcag cagtgtatcg gatctggcac ttgctttctg gcaaatagct
      661 gaaaaagtgt catcagtgat acaggtcagt ccgtcccagc ctaattggct aatgcatttg
      721 gccgttcttg aagtgtcttg gtatctggaa tatgttttgc ctttcatcgg ccaagttgca
      781 gccgggcagc tttaatctcg tcggtgaaca ggaggttagt catgaagtcg caacgcgtca
      841 ggtgggaaga acagagtttt tggcaactag tatttggcca aagtaaagtt aacggagcca
      901 aatatgatgg cgagaatcta accacatctc cctggcgttg gcctactaac acttatctgc
      961 aacggcgccg gcagctgttg aaaaacagcc aagcgttcga tcagtccggc caaagtgaag
     1021 ccggcgtttt ttacgatagc gtctacgata ctggcaacca tgccattgcc gagtttcgcg
     1081 actcgggttc gttgcggaat aattgagaat ttgatcaaag ttctattctg ccattcgctg
     1141 caatcttgcg ccggataaga tccggaggcc gattcgaaac gagtcggccc acttctgctt
     1201 ggcgccaaaa cgtgagctgc cgtcatagca cagcgcggcg ctgaatgcag cgaacatctc
     1261 tcgtttagat catttactta gggatctgcc cggcgctgtg tcgattggat gcaaagcagg
     1321 gtgcacgttc tatgtcggtc aaaaaaatac tgcagccgga taagaactgc tggacaatca
     1381 gcgaagtcac cgagtcgggc ctgctcatcg atggcaggga ctattaccgg gccttttatc
     1441 aggccgcccg aagtgcggag gattatattc tcatttcggg ctggcagttc gacagcgatg
     1501 tcgcgctgct tcgcggcagc gatgcagact atgctccagc ctccgtacag ttcctcacgt
     1561 tcttgaatga tctctgcgag caaaaccaac gattacacat ttacattctc gcttgggatt
     1621 ttacagttct ctatagcctc gaccgtgagt ggttccagga ttggtatttc aactggacta
     1681 ccaacgagag gttcacgttt tgctttgact gctgcgagtg ctttggcgcc gcgcatcacc
     1741 agaaatttgt cgtgattgac ggcaagctcg cctttgtagg ggggctcgat atttgttcag
     1801 gccgctggga cgatcgcgac catccggtcg acagtccggc tcgtgtgaat tccgaccaaa
     1861 acggctacgc gccgttccat gacatccagt cgtaccacgt cggcccggcg gcgaagaagc
     1921 tggccgagct gtttgtcgcc cgctggagat gcgtcaccac cggggatctc gagctggcgc
     1981 aatcatcttc gaattaccag atcccatttc gacccaccgt ggcgatcccc gcagagtcga
     2041 ttgcgattag ccgaacccag agtggaactg tggggcaaca aagaccgatt caggaaatcc
     2101 gccgtttatt tctcgatgca atcgatgccg cagaggattt gatctatgtc gagaatcagt
     2161 atttcagctc gaacgcgctg tacaaagctc tggtggatcg aatgatgaat tccgggcgct
     2221 ctcatttgga gatcgttctg atcatcgcca aagatgccga agcttttgtc gagcagcttt
     2281 cgatcggagt cgtacaggaa aaactccttc gcgaactcca gagtgttgcc gcggagacag
     2341 ggcactcatt cggcatctat tatccggctt cggtttgcgg cgatggcagg gaaagcgcca
     2401 cgtatattca ctcgaagctt ttcctggtgg atgaccgttt cctgagcgta ggctcggcca
     2461 atatgaacaa tcgcagcctg ggactcgata ccgaattaaa cgttgcgtgg gaggcgcctg
     2521 cggcggatag tgagcttggt cgcgccattc gcgctgctcg gattgacctt ctggccgaac
     2581 atgtaggagc gcgggaagct caggtcctgg aaaatctaac ccggagccgc ggcttggtaa
     2641 agtacttaaa caccctggca gacagcggtt cttatcgtct ccgccaccat ccgctcgata
     2701 ttgtctctga agattatctg tgggtcacct ctgtgctccc ggagggattg ccgtttgatc
     2761 ccgagggtcc cttctatcag gaagaccgct acgaagggat ttccgccact gaggattcgt
     2821 tttttagtcg cggcgtgagc agtttgaaaa actggttgct gggcctgggt cagacgttat
     2881 ctccacgttg aatcgcagac gattgagtcc ggttaacctg accaccctgc cgtataggcg
     2941 gaattagcca cgcagtcgtg cagctgaaag gggattgaag ctgtgcagcc caggaaaccg
     3001 tttacgaatt cgctgcaacg gcggcgccaa tttcgcacga ggatgtcgca ggtgtaattg
     3061 cctccccgga caaagcggcg tttctaaagt tgctttcggc ggcttcttgt ccagcgcggc
     3121 aattatcaca gcgactgcaa tcggcttcga cctcctcgcc aaaatagtcc agtagataac
     3181 gcatgcgaca ggtggtgatc tgaccaaaaa gcatcattgc gtctaaacgt tgccggtcgc
     3241 cgttgtgacg ctgctcgtat tcttcgagaa acgtggcgaa ctcttgatcg gtattgaagt
     3301 cgcggatttt gctcacgtgc ggtctgcgct gaattattcc cgtgctttcc aacagcgcga
     3361 gaataacctt gactcgtttc gccgcgaggt cggtaacctc agccagccat tgctcgggag
     3421 tgcggtctcc caaatcttct cgtcggggca tctggtccaa tgtccgatac agcgccaagg
     3481 actcatcggg gcgcggatat ttgccgccca gaaaatagga ttggacgcgg cgatcctcaa
     3541 gccgataaaa caggatcgcc cgggccggct tgccgtcgcg gcccgcccgg ccggtctcct
     3601 ggacataggt ttcgactgag tcgggaacgg cataatgaat aacgaaacga atgtcctgct
     3661 tatcgatgcc cagaccgaac gctttggttg cgaccatcac atcgatctcg ttggccataa
     3721 aggcttgttg gttggcttcg cgcgccggca tcttcatccg accgtgatac tggccggcgc
     3781 gaaagccttc gtcgatcaac catttgcata aagcatcgac ctgtttcacc gtcggcacat
     3841 aaataatccc cgcgccgccc gattgttcct gtagaacttc ttttagccgg gcttgtttga
     3901 catcctcgtt gacggtgcga atgacctcaa atgcgaggtt ttttcgttcg ataccgatgc
     3961 taatcacctc ggcgtccggc agatcaagct gctttaatat atcgactgcc acttcgtcag
     4021 cagcggttgc tgtcagggcc ag
//
LOCUS       DASUTG010000554         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_86374, whole genome shotgun sequence.
ACCESSION   DASUTG010000554 DASUTG010000000
VERSION     DASUTG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86374"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF089_12745"
     CDS             complement(<1..288)
                     /locus_tag="VF089_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174878.1"
                     /translation="MLTYGQALRAIGQDLEGHGALVYEIKVSDAEYRVQCDCDLPPPD
                     NLLSLCYTSRILEQIDFMGKLKRTGVFSVNSPESLPGTLRSLGGYVDREKGR"
     gene            complement(307..936)
                     /gene="nthA"
                     /locus_tag="VF089_12750"
     CDS             complement(307..936)
                     /gene="nthA"
                     /locus_tag="VF089_12750"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX6174879.1"
                     /translation="MSTSGDEHGHEHPRHELSPQAKRIYAIRDLLLEKGVITKEDIQS
                     QVEYQEARSPANGAKIVARAWVDPSFKARLIADPKAACAELGIDASALNEFVVLENTD
                     KVRHMVVCTLCSCYPRPILGRPPDWYKSFNYRQRSVNDPRGVMREFGLVLPDDVEVRV
                     HDSTADIRYLVLPLRPKGTEGLTEAELAKLVTRDSMIGVMDALPKLPEP"
     gene            complement(920..1249)
                     /locus_tag="VF089_12755"
     CDS             complement(920..1249)
                     /locus_tag="VF089_12755"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX6174880.1"
                     /translation="MIHPATGRFRPGDAVRVLNEHRPGHIRTPYYIRGKTGWVERVYG
                     DFLNPESLAFGGDGLPRRTLYLVAFNQADVWGPAQAPGDKLFVDIYDHWLEPAAGYGG
                     HKDEHVR"
     gene            complement(1246..1557)
                     /locus_tag="VF089_12760"
     CDS             complement(1246..1557)
                     /locus_tag="VF089_12760"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174881.1"
                     /translation="MQRGHHDIGGLPGAGPIDKSQHELADWEILADAVNQALGAKGVK
                     RTDEMRRAREEMDSEAYRKMNYYERWIASIETILIEKGILKKDEIDKKVVEFEKKWGE
                     P"
     gene            complement(1571..>2309)
                     /locus_tag="VF089_12765"
     CDS             complement(1571..>2309)
                     /locus_tag="VF089_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX6174882.1"
                     /translation="DPVPANAIDWRGKAWDPKSGHPAAHPNSRFTVSLKQCPTYSPEW
                     EKPEGVPISAILFGARRNKVVPLVYQSFDWQHGTFLGATLASETTAAATGAVGVVRRD
                     PMAMLPFCGYNMGDYWGHWLSIAKRATNPPKIFRVNWFQRNDQGKFIWPGFGENLRVL
                     RWVIERSKGGGQADETAIGYVPKASSLNGDGLDVSKSDLDQLLTVERDAWKANLKSQR
                     EYFDKFGDHLPAGIKEQHRALAERLKG"
BASE COUNT          480 a          690 c          597 g          542 t
ORIGIN      
        1 ccggcccttc tcccgatcta cataccctcc caggcttcgg agagtgccag ggagcgactc
       61 agggctgttc acagaaaaaa cgccggtgcg tttcaacttg cccatgaaat ctatttgctc
      121 gagaattctg ctggtataac aaagagacag aaggttatct ggcggcggca gatcgcagtc
      181 acattgaacg cggtactcgg cgtcgctgac cttgatctca tagaccagcg cgccgtggcc
      241 ctccaaatcc tgaccgatgg cgcgcagagc ctgcccatat gtcaacatta aaacaagaag
      301 aagggattaa ggttccggca atttcgggag tgcatccatg acgccgatca tgctgtctcg
      361 cgtgaccagc ttcgctaatt ccgcctcggt cagtccttcg gtcccttttg gacgaagtgg
      421 caataccaaa tagcggatat ccgctgtgct atcgtgcacg cgcacttcaa catcgtcggg
      481 aagcaccaga ccgaattcac gcatgacgcc gcggggatcg tttaccgacc gttggcgata
      541 attgaagctc ttgtaccaat cgggcggacg acccaaaatc ggccgcgggt aacaggagca
      601 cagcgtgcac accaccatgt ggcgaacttt gtcggtattc tctagaacga caaattcgtt
      661 aagcgcgcta gcgtcgattc ccagctcggc gcaagcggct ttaggatcgg caatcagacg
      721 cgccttgaat gacgggtcga cccaggcgcg cgccacaatc ttggcgccgt tggccggaga
      781 acgcgcttcc tgatactcga cttgactttg aatatcctcc ttggtgataa cgcctttctc
      841 cagcagtaag tcgcggatcg catagattcg cttagcctgc gggctaagtt catgccttgg
      901 atgttcgtga ccgtgctcgt cacctgacgt gctcatcttt atgtcctccg taccctgccg
      961 cgggttccaa ccaatgatcg taaatgtcca cgaacagttt atcccccggc gcttgcgccg
     1021 gtccccagac atcggcttga ttgaacgcaa cgagatagag cgtccgcctc ggcaacccgt
     1081 ccccgccaaa ggcaagcgac tcggggttta agaagtcgcc ataaactcgc tcgacccaac
     1141 ccgtcttgcc gcgaatgtaa tagggtgtgc ggatgtgtcc cgggcgatgc tcgtttagca
     1201 ctcgcacggc atcgcccggc cggaatctgc cggtggccgg gtgaatcatg gctcgcccca
     1261 ctttttttcg aattcaacga ccttcttgtc gatctcatct tttttcagga tgcctttttc
     1321 aatcaggata gtttcaatgc tggcgatcca acgctcataa taattcatct tgcggtacgc
     1381 ctcggaatcc atctcctcgc gcgcgcgccg catttcatcg gttcgcttga ctccctttgc
     1441 gcccagagct tgattaaccg cgtctgccaa aatttcccaa tctgcaagct cgtgctgcga
     1501 tttgtcaatc ggcccggccc cgggcagccc gccgatgtcg tgatgtcctc tttgcatgac
     1561 tttactccaa tcaacctttc aggcgctcgg ccaaggctcg gtgttgttcc ttgatgcccg
     1621 ccggtaggtg gtcaccgaat ttgtcgaagt attcgcgttg gctttttaga ttcgctttcc
     1681 atgcgtcccg ctccacagtc aataactgat cgaggtccga tttagacaca tcgagtccat
     1741 caccgttgag gctcgaagcc ttgggaacgt agccgatcgc cgtctcatcg gcttggccgc
     1801 cgccctttga gcgctcgata acccagcgca gtacacgcaa gttttcaccg aagcccggcc
     1861 agataaactt tccttgatca ttcctttgaa accaattgac gcgaaaaatt ttcggcggat
     1921 tggtcgctcg cttggctatg gatagccaat ggccccaata gtcgcccatg ttatacccac
     1981 aaaagggtag catcgccatc ggatcgcgcc gcacaactcc gaccgcaccc gtggccgcag
     2041 cggtagtttc cgaggctaga gtcgcaccta agaacgttcc gtgttgccag tcaaaagact
     2101 ggtaaaccag tgggaccacc ttgttgcggc gggcgccgaa taaaattgcg ctgatcggca
     2161 ctccttctgg tttttcccat tcgggagagt acgtaggaca ctgcttaagt gacactgtga
     2221 atcgggagtt cgggtgcgcc gctggatgac cgctcttcgg atcccaggcc ttgccgcgcc
     2281 agtcaattgc attggccggt acgggatca
//
LOCUS       DASUTG010000555        13595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1122173, whole genome shotgun sequence.
ACCESSION   DASUTG010000555 DASUTG010000000
VERSION     DASUTG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122173"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF089_12770"
     CDS             <1..779
                     /locus_tag="VF089_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174883.1"
                     /translation="TARRCMGLCFVALLLSSLIFVTGVSAAQEDNPLPPGMYRLEMIL
                     ASIARLPFFGLSKSASRSVSLIEVRSDPTGLLQRHEVCDFHVLQGTAMIKMVFPDKFI
                     AALAKHSYPIQVEKDAEGWRYRADLGIERIGYKPTLTESSLPTKLDDASVYDWDGDGH
                     PGATLKISVPLLPDGELYVVQRGQSILNGRITRPGRIEGSIEVRSFEHRVLGAWPGFL
                     NRSPEIVPDPDASRFTMIAIPQDSVCETLRGASRQPKQKD"
     gene            848..1945
                     /locus_tag="VF089_12775"
     CDS             848..1945
                     /locus_tag="VF089_12775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6174884.1"
                     /translation="MAKTSQCRRVETSDRPSSARITVLLPVLNEATRIANSLETLIAQ
                     PEEVSEILVIDGGSTDATRAVTENYRRRDDRVRWIDASPVGPRWIGKAWNLNLGLARS
                     NPASQWILCVDADVRVSPKLSRSLISHAARTGVSTFSVATRQHLSGRLEALIHPPLLT
                     TLVYRFGQPGRSYGTIAQVQANGQCFFSRREILLRAQAFAAARSSLCEDITIVRRLVE
                     RGEAVGFYETYGLVEVSMYRDWRETWTNWPRSLPMRDQYFGWRQAAGLLEVLFIQGLP
                     LPMLILSWGVSAPKLFRTLNALLLALRVGVLFGTTRAYDRRPWTYWLSPVCDFAVAVR
                     LIQSALSRQHSWRGRSYVRRSAGKFEPKESC"
     gene            2026..2703
                     /locus_tag="VF089_12780"
     CDS             2026..2703
                     /locus_tag="VF089_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX6174885.1"
                     /translation="MARAVAYGRLRTTCSGIGHIPSTGPVLIIARHYHHLYDGLALFA
                     ALPRQFHILVTLDWVQSAAMRTLMTQLTRLARWPVVLRADALLHKNDCGAPPFRHAFS
                     VADVERYQRRALKDSVQLLVENRLLVVFPEGYPNIDPAYTPKADNHGFLPFRRGFLAI
                     ARAAERRLGASLPIIPTGLQYSSGNTWQAHVRFGEPTKRESFASDNELIRYCEEKVRQ
                     LSSQVPL"
     gene            complement(2753..3007)
                     /locus_tag="VF089_12785"
     CDS             complement(2753..3007)
                     /locus_tag="VF089_12785"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003290524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX6174886.1"
                     /translation="MANKNSQELKFEEAIANLQQVVEQLESGDLSLEDSLAAFEKGVG
                     LVRYCNQKLSEVEQKVELLVKDKEGQLQLKAFGNGPTPDS"
     gene            complement(3024..3920)
                     /locus_tag="VF089_12790"
     CDS             complement(3024..3920)
                     /locus_tag="VF089_12790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6174887.1"
                     /translation="MIVRAVVLLVCFSASAVHATEPFALSWPTRLKIPQGEIVEIKVD
                     GEGLSSVEARWAERNLPFYPVAPGSFAAIIGVDLEAKPRVSKITITATNDAGRQRRAQ
                     TIVQMLSKSFREESFKVAPGFDQLSPEALELIRIERADFALAFSTSAAERIWEGPFIR
                     PVPQESSSSFGYRRIINGKPRAPHTGADLRAPVGTEVLAANHGRVVLTGNYFFAGRSI
                     VLDHGGGLYTMYFHLSEVKVDAGAMVRRGDVIALSGMSGRVTGPHLHWAARLADARVD
                     PMELIHKISIGQSQKSGTAMAD"
     gene            complement(3922..5142)
                     /gene="xseA"
                     /locus_tag="VF089_12795"
     CDS             complement(3922..5142)
                     /gene="xseA"
                     /locus_tag="VF089_12795"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX6174888.1"
                     /translation="MRKEAGLPLFTATPKYLTVSQLNQLVRVTLETRLSAVWVLGEIS
                     NFRIPPSGHCYFTLKDDKSQISAVMFRRQGQNLAFVPENGMEVLCFARVSLYTARGDL
                     QIYVESLEPRGQGALYLAFEQLKKKLAQEGLFATERKRPLPFLPDKIGIVTSDTGAAL
                     HDMLRIIRDRYPDRRVVVRPVKVQGLGSAKEVAAAIADLCTTGVDVMIVGRGGGSLED
                     LWAFNEEVVARAIFACSVPVISAVGHEVDFTIADFVADQRAPTPTAAAEMVVPCKKDL
                     LAELQVIENQLLRCMQSKIDNAQEICRGLSKRLSDPGRKLREKQQHLDDLSVDLQRRF
                     LGRLNHFKERLRRESSRLDALSPLAVLDRGYSITHKLPDEVIVKTTDELDLGDLVRVT
                     LAQGKATCRVEGKD"
     gene            complement(5212..6570)
                     /locus_tag="VF089_12800"
     CDS             complement(5212..6570)
                     /locus_tag="VF089_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX6174889.1"
                     /translation="MRSMTKHGVSLTILLVGIALGFVLAGQWVPNVSAVPRQDYETLE
                     TFTNILSIVKKNYVEDVETKNLVNGAINGMLTSLDPHSAYLTPDLYKELQMDTQGRFG
                     GLGIEITVKNGVLTVVSPIEDTPAFKAGVKPGDMIFKIEEDFTKDMTLVDAVKRMRGP
                     KGSKINLSIKREGNPELINLSLIRDTIRVQSVRNRTLEPGYGYIRLAQFQERSDRDLQ
                     KALEKMAAEKGGLKGLVLDLRNNPGGLLTQAVRISDIFLDSGLIVYTDGRIESQKQKF
                     FAQKDSSSMDFPLVVLVNGGSASASEIVAGALQDHKRAVVLGTKTFGKGSVQTILPLD
                     DNSALRLTTARYFTPNGRSIQATGIVPDITVESTPQQDGKLDDRKRPSLREENLPGHL
                     QNQQQNLSPQEQLDREREKQIPSSAPTGDETIDNDPQLKRALDLLKSWDVFKQVVQKK
                     AA"
     gene            complement(6642..7790)
                     /locus_tag="VF089_12805"
     CDS             complement(6642..7790)
                     /locus_tag="VF089_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6174890.1"
                     /translation="MARLATVAVCLMAGLPAALAYSASADRDLEGIKKKIESEKRVLS
                     QVNKKEGSVIQSLSQVESDLERKNKQLKQSTARLEVVVSELQKKEAEALRMRESLEQR
                     KAYLARRAEALYRWQRSGSPLVIFNGDLSLSSLLKRKYYLQRTVAFDREMVQSLNEQA
                     IHQERLAQELARKRQDVDDQRRDLAQVKEAVQKEAQKKRELLASIREEKESRQRALKD
                     LEQAAGRLQRMIEEISRRSAARSKEASAGAGLESMRGKLDWPVKGEVIGGLGKAKHHE
                     FSTEVYRKGLEIEAPVGEEIRAVEKGKVVFADRFSGYGKMLIIDHGDRYYTVYAHISD
                     FLKKNGDSVRRGEAVALVGDSDSLAGSKLYFEMRKDGRSIDPAPWFRR"
     gene            complement(7778..8686)
                     /gene="ftsX"
                     /locus_tag="VF089_12810"
     CDS             complement(7778..8686)
                     /gene="ftsX"
                     /locus_tag="VF089_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX6174891.1"
                     /translation="MSWSHLAFVARRVRQSLWELAWSHVLTSGTMATSLFVFGVFILI
                     QENLQHLLNGWGDQIQINTYLDNDVDLDRLEALMSRVRAMPEVEAFRHISKEQAWMDF
                     QTALGAQSAVLEGLPPDVLPASFEITVKPAYRDPLQIEDLAGRLGKINGIAAVEYPQQ
                     WVDRLNLFVLAVQWAKWALGGILFIATFFIVGSTVRLALLARKDEIEIMQFVGASEGL
                     IRAPFVVEGMLQGSVGAGISIFCLWLCYLLLQQYMVDQMGLFGSNMQLRFLDPASIAM
                     IVAIGLLLGGFGSLFSLRRFLKTWRG"
     gene            complement(8683..9366)
                     /gene="ftsE"
                     /locus_tag="VF089_12815"
     CDS             complement(8683..9366)
                     /gene="ftsE"
                     /locus_tag="VF089_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX6174892.1"
                     /translation="MIELSHVTKTYPPGSPALVNITLSIETGQFVFLTGPSGAGKTTL
                     LKLLFREEEPTEGQILIDHQNVTRLHTRGVARLRRRIGLVFQEFRLLPRLTVLENVAL
                     AAQVVGVSRREAQIKAVRILKELGLEKPQAKPLMLSAGEQQRVAIARALINEPKLILA
                     DEPTGNLDPGLAEEIMRIFLKLREAGTTLIVASHNLDLIKRYGSRILSLRQGRLVDDL
                     ERVTEAHAS"
     gene            9519..11735
                     /locus_tag="VF089_12820"
     CDS             9519..11735
                     /locus_tag="VF089_12820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6174893.1"
                     /translation="MTHQQKSIEKFRIVLIKPSKYDDDGYVIRFWKGVLPSNTLNVLH
                     GLTEDVIQRGVFGDLPIEVTTFDETAEKVPLHKIIRWSGEPNTRLLVCMVGVQTNQFP
                     RALDMARVLRAEGITVIIGGFHTSGTVNMLGEQEPDMQELFREGICAVSGEVEGKWDM
                     ILADFLEGKLKPLYSFAQDLQSLVDIEDSPLPKTSSKTMEHFAYPSFGTADTSRGCPF
                     ACSFCTIINVQGRKMRERSPQSIAAMMRRNYRDHGVSFYFFTDDNFARKKLWRETFEE
                     IIKLREEGLDVTFMMQVDLARKPKDFVSLAAKAGCTQVFIGMESVNPQNLESEGKKQN
                     KVEEYREIIQEWHQVGVVVHTGYIIGLPWDSKEQVPNDIRFLIDEVGPDQASFFMLTP
                     LPGSHDHREMKRRGDWMDPDFNKRDSFHATIKHPLMTEEEWTDCYEKAWRTFYSKENM
                     IRVLSRWTDNPRNYWNLMSIFLWYKNAALIEKQHPMVAGFFRLKDRKARRPGYAIDSI
                     PVHAWKRSKEITHLLVNWAKFLKEIEEVWLQTRKKSDTEERWVEQIQKIQTDIWQALR
                     IGELQKLYSNARETLSGQARTLLDPLEELSSRILYNRSDLNRFLKQWEHLRNRIQEIR
                     SIETDAARSCLDEMHNLQSSVRLTDRVSEWQEAYTRLRGSLPSGASLRLIKFDAINYR
                     VVYSRQELQRIWSHTIHNARQMKLWQIRPWRVTRAMIQDFSLTTSFAANFKAFARR"
     gene            complement(11761..12594)
                     /locus_tag="VF089_12825"
     CDS             complement(11761..12594)
                     /locus_tag="VF089_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174894.1"
                     /translation="MSNDLYVAFREAVDRPEDEIDLGHAAVAIATQEYPDLKIDDCLS
                     QLDRLGQAVELRMGDEKNPYRIIAALNTVLFKELGFQGNRSEYYDPKNSFLNDVITRK
                     KGIPISLSVIYMEVARRVGLSLVGVGFPGHFLVKYEDDAVEILIDVFNGGEIRAREDL
                     ERMLQQLYRGQVSYQAGFVAALGKRDILRRMLNNLKWIYLERGEPLKTLSVLDQLVIL
                     DPRSASEIRDRGLLYITLERYAQALEDLEGYLRLAPGADDVEMVKAQIESVRQRYVQI
                     H"
     gene            complement(12607..>13595)
                     /locus_tag="VF089_12830"
     CDS             complement(12607..>13595)
                     /locus_tag="VF089_12830"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6174895.1"
                     /translation="THPLDATDGAKDILPKADVILALDVADLYGSLTTVSKQTRDCVY
                     VTSPSVKIITISMHDMLVRSWAGDYQALQPTDIPLAADTSVAVPELTRLCRELLGNDS
                     KRKSVIEARQKELAEKSKSRRAKWLADAQAKASLQNISTAFLAYDLGEVIKREDWVLV
                     NGTSNGWARRLWEFTKPNQYLGASGGAGVGYGASAAMGAALALKGSGKVAVDIQSDGD
                     LLMTSSCLWTAAKHRIPLLMVLHNNQSFYNSEEHGIEVAKFRNRPVENAGIGTHVDDP
                     EVDYAKMAQSFGVHGEGPIRRTSELRPALERALKYVKEKQLPALVDVISEPR"
BASE COUNT         3186 a         3769 c         3434 g         3206 t
ORIGIN      
        1 aaacagcccg acgctgcatg ggcctatgct tcgtagcgct cctgctaagc agcctgattt
       61 tcgtgactgg cgtctccgcg gcacaagaag acaatcccct gccgcctggg atgtatcgtc
      121 ttgagatgat tttggcatcg atagcacgct tgccattttt tggtctgtcc aaatccgctt
      181 ccagatcggt atctttgatt gaggtcagaa gcgatcccac tggtttactg cagcgccacg
      241 aggtatgcga tttccatgtc ctgcaaggta ccgcgatgat caagatggtc tttcccgaca
      301 agttcattgc tgcgttggcc aaacactcct atcccatcca agttgagaaa gacgctgaag
      361 gatggcgcta tcgcgcggat ctagggattg aacgaatcgg ctataaaccg actttgaccg
      421 aatccagtct gccgacaaag ttagacgatg cttcggttta cgattgggat ggggatgggc
      481 acccgggcgc gaccctgaag atatccgtgc cgttactgcc cgacggagag ttgtacgtcg
      541 tacagcgagg ccaatcgatt ctcaacggcc gaatcactcg gcccggaagg attgagggca
      601 gtatcgaggt gcgcagcttt gaacatcggg tcctgggcgc ttggccaggg tttctaaatc
      661 gctctccgga aattgtgccc gatccggacg cgagccggtt tacgatgatc gctattccgc
      721 aggacagcgt atgcgaaacc ttgcggggcg cctcccgcca gcctaagcag aaagactaaa
      781 aggcattcgt ttgtttttct tcgtgggact attgcaaacg atacttgcag cgcgagtatt
      841 ttttcggatg gcaaaaactt cgcaatgccg gcgggttgag acatccgatc ggccctcatc
      901 ggcgcgcatc actgtcctcc taccagtcct caatgaagca acccggattg caaattctct
      961 ggaaaccctc atcgctcaac cggaggaagt ctccgagatt ttagtaatcg atggcggatc
     1021 cacggatgcg acccgagcag ttacagaaaa ctaccgccgg cgcgatgatc gcgtgcgctg
     1081 gatcgatgcc agcccggtcg gcccacgctg gataggcaaa gcctggaatc ttaacctcgg
     1141 tctggctcgc tccaatccgg caagccagtg gattctttgt gtcgatgccg acgtgcgagt
     1201 ctctcctaag ctctctcgct ccttaataag ccatgcggcg cgcaccggag tatctacatt
     1261 ttcggtagcc acgcgccagc atctatccgg aaggctggaa gcgttgatcc accctccttt
     1321 gttgaccact ttagtttacc gcttcgggca acctggtcgc tcttatggaa cgatcgctca
     1381 agtacaagcc aacggacagt gtttttttag ccggagagag atcctactcc gggcgcaagc
     1441 ctttgccgcc gctcgatcgt ctctttgtga ggatattacg atcgtgcggc gattggtcga
     1501 acggggcgaa gcggtaggct tctacgaaac ctacgggctc gtggaagtat caatgtatcg
     1561 cgattggcgt gaaacctgga caaactggcc aaggtcgctg ccgatgcgtg atcagtactt
     1621 cggttggcgg caggcggcgg gcctgctgga agtgcttttt atccaagggc tcccactgcc
     1681 gatgctcatt ctgtcttggg gtgtaagcgc tccgaaactg tttcgcaccc tgaatgcgct
     1741 tcttttagcg ctacgagtgg gagttctgtt cggcaccacc agagcgtatg atcggcggcc
     1801 ctggacttat tggttatccc cggtctgtga ctttgccgtg gccgttcggc tcatccaaag
     1861 cgctctgtca cgacaacaca gctggcgcgg ccgttcttat gttcgccgaa gcgctgggaa
     1921 attcgagcca aaagaaagtt gctaaacggt atcctacggc aggacaacgc catgacgtct
     1981 ccgggatccc ttactgcact tgcaacggca aagtcgatgc ggctcatggc tcgggccgtc
     2041 gcgtacggcc ggctgcggac aacctgttcc ggcatagggc acatcccttc gactggacct
     2101 gttcttatta tcgcccggca ttatcaccat ttgtacgatg gtctggcgct ctttgccgcc
     2161 ctgccgcggc aattccatat tctcgttacc ctggattggg ttcaaagcgc ggccatgcga
     2221 acgctaatga cacagctaac ccgcctggcg cgatggcccg tggttctgcg agccgacgcc
     2281 ttgctccaca aaaatgattg cggcgcgccg ccttttcgcc acgcgttttc agttgcggat
     2341 gtggagcgat accagcgccg ggcgctcaaa gactcggtgc agttgctcgt ggagaatcgc
     2401 ttgctcgtgg tttttcccga gggataccca aacatagatc cagcatatac gccaaaggca
     2461 gataaccacg gcttcctgcc gttcaggcga ggattcctcg cgatcgcccg tgccgccgag
     2521 cgccgcctcg gcgcatcact gccgattatt ccgacaggtc tacagtacag ctcgggaaac
     2581 acttggcagg ctcatgtgcg gtttggcgag ccgaccaaac gtgaaagctt cgcgtcagac
     2641 aatgagttga tccgctactg tgaagagaaa gtaaggcagt tgtcgtctca ggttccccta
     2701 tgaatctacc ggcctaagga tgggttacag ccgttcgcaa tcactggcct gactaggaat
     2761 ccggcgtcgg gccgttgccg aatgctttta gctgcaactg cccttccttg tcctttacca
     2821 gtagctccac tttctgctcg acctcgctca gcttttgatt gcagtagcgc accagtccca
     2881 cacctttttc aaaagccgcc agtgaatctt ccagggaaag atcccctgat tccaactgct
     2941 cgaccacttg ctgtagattc gcgatggcct cttcgaattt aagttcctgc gaatttttat
     3001 tcgccatacc tgctccgtgt gaattaatcg gccatagcgg ttcctgattt ttgcgattgg
     3061 ccgatggaaa ttttatggat caattccatc ggatcgaccc gggcatcagc caagcgcgcg
     3121 gcccaatgta agtgcggacc agtcactcgt ccgctcatcc ccgacaaagc aatcacatca
     3181 ccacgacgca ccatcgcccc ggcatcaact ttgacttccg acaaatggaa atacatcgtg
     3241 tagagtccgc caccatgatc cagaacgatg cttcgaccag cgaaaaaata gttgccggtt
     3301 agcacaactc ggccgtgatt ggccgccagc acttcagtac cgacgggagc ccgcagatcg
     3361 gcgccggtat gaggcgctcg cggcttcccg ttgatgatcc gacgatagcc aaaagacgaa
     3421 gaggattcct gcggcaccgg ccggatgaag ggaccctccc agatacgttc ggccgccgat
     3481 gtcgagaatg ccagcgcaaa atctgcccgt tcgatacgaa ttagctcgag cgcctccgga
     3541 cttaactgat cgaaacctgg ggccacttta aacgactcct ctcgaaagga cttcgacaac
     3601 atctgtacga tcgtttgcgc tcgccgctgt ctgccggcgt cgttggtggc ggtaatcgtg
     3661 attttagata ccctcggttt ggcttccaaa tcgacaccaa tgatcgctgc aaacgagcct
     3721 ggtgcgaccg gatagaacgg gagatttctt tcggcccatc gcgcttcgac cgaggatagg
     3781 ccttcgccat caacttttat ctcgacgatt tcaccttggg ggattttgag gcgcgtcggc
     3841 cagctcagag caaaaggctc tgtcgcatgg acagccgaag ctgagaaaca gaccaggaga
     3901 acaactgccc gcacaatcat gtcagtcttt gccttcgact cgacaagtgg ctttcccctg
     3961 agccagggta acccgcacca agtcaccgag gtccaactcg tcagtggtct ttacgattac
     4021 ctcatccggc aatttgtgcg tgatgctgta gccgcgatcg agaacagcga gcggactcag
     4081 cgcatccaag cggctactct cgcggcgcaa gcgttcctta aagtggttca gtcgacccaa
     4141 gaaacggcgc tgcagatcca cagaaaggtc atcgagatgt tgttgttttt cgcgcaactt
     4201 acgtcccggg tcggataatc ttttgctaag cccacggcag atttcctggg cgttatcgat
     4261 tttgctttgc atacatcgaa gcaactgatt ctcgatcact tgcaattccg caagcaaatc
     4321 ctttttacaa gggacaacca tttccgcggc cgcggtaggt gtcggcgcgc gctggtcggc
     4381 aacaaagtcc gcgatcgtga aatcaacttc atgaccaacg gcggagatca ccggcaccga
     4441 acaggcgaag atcgcccggg caactacttc ttcgttgaaa gcccagagat cttccaatga
     4501 gccgccaccc cgcccgacaa tcatgacgtc gacaccggtc gtacatagat ccgcaatcgc
     4561 tgcggcaacc tcttttgcag acccgagacc ttgaactttg accggccgaa caacgacacg
     4621 gcgatcggga taacgatccc ggataattct cagcatgtcg tgtaacgccg caccggtatc
     4681 cgaggtcacg attccgatct tatccggtaa gaaaggcaag ggccgtttgc gttcggtcgc
     4741 aaacaagcct tcctgagcca gctttttttt gagttgctca aatgcaaggt agagcgcgcc
     4801 ttgacctcgg ggttcgagcg attcgacata gatctgcaaa tctccgcgcg cagtgtaaag
     4861 actgactcgt gcaaaacata aaacctccat gccgttctcc ggcacgaagg ctaggttctg
     4921 accctgcctt cgaaacatca ccgctgagat ctggctcttg tcatccttga gggtgaagta
     4981 acaatgcccg gaaggaggga tacgaaagtt agaaatttcc ccaagcaccc aaaccgcact
     5041 caagcgagtt tccaatgtca cccggaccag ctggttgagc tggctgacag tgagatattt
     5101 aggggtggcg gtaaatagcg gtaaacccgc ttctttcctg atcacgatct acttccacaa
     5161 aaaaattagc cctccggggg agggctaatt aacatcacag taagtgaaaa tttatgcggc
     5221 cttcttctgt acgacttgct tgaagacatc ccagctcttt aacagatcca gagcacgctt
     5281 cagttgagga tcgttgtcaa tcgtttcatc tccagtcgga gccgaagagg gaatctgctt
     5341 ttcccgctct ctgtctagtt gctcttgtgg cgacagattc tgctgctggt tttgcaggtg
     5401 gccgggcaag ttttcctcgc gcaagctcgg cctcttgcga tcgtcaagct tgccgtcctg
     5461 ctgtggcgtg ctctcgacgg tgatgtcggg aacgattccg gtggcctgaa tagatcggcc
     5521 attaggagtg aagtaacgcg ccgtagttaa tcgtagcgcc gagttgtcgt ccagaggcag
     5581 aatcgtttgc actgaaccct tgccaaaggt tttggtgcct aagacgacgg cgcgcttgtg
     5641 atcctgtaag gcaccggcaa cgatttcgga cgcgctggcg ctgccgccgt ttacgaggac
     5701 gaccagcggg aaatccatgg aggaggaatc tttctgagcg aaaaacttct gcttttgaga
     5761 ttcgatgcgc ccatcggtgt aaacaatcaa gccggaatcc aaaaagatgt cggagatcct
     5821 aaccgcttgc gttagtagac cgccgggatt gtttcgaagg tctagcacga gccccttcaa
     5881 accgcctttt tccgcggcca ttttctccaa tgccttctgt aaatcacggt cactgcgctc
     5941 ctggaactgt gccagacgaa tatagccata cccgggctcc aaagtccggt tacgaacgct
     6001 ttgaacccgg atggtatcgc ggatcaagct gagattgatc agttccgggt ttccttctcg
     6061 tttgatcgaa agattaattt tgctgccttt cggaccacgc attcttttta cggcatcgac
     6121 cagtgtcatg tctttggtaa aatcttcttc tattttgaag atcatgtcgc ctggcttgac
     6181 tccggctttg aatgccggcg tatcttcgat tggtgaaacc accgtgagca caccgttttt
     6241 aacagtaatc tcgattccaa gaccgccgaa tctcccctgg gtatccattt gtaattcttt
     6301 atagaggtcc ggcgtcagat aagcactatg aggatcgagc gaggtcagca tgccgttgat
     6361 cgcgccgttg acgagatttt tcgtttcgac gtcctcgaca tagtttttct tgactataga
     6421 gagtatgttg gtaaaggttt ctagggtttc atagtcctgc cggggcaccg ccgacacatt
     6481 cggcacccac tgtccagcta agacaaaccc cagagcaatt ccaacaagta gaattgtaag
     6541 actcacacca tgcttggtca tagaacgcat aggatcccac ctcgccagtt ctggccatct
     6601 ttttgtgaaa caatactata tcaacggttt tgaaatagct attatcttcg aaaccagggg
     6661 gccggatcga tcgatctgcc gtctttgcgc atctcgaaat aaagcttgga gcccgccagt
     6721 gagtcgctat ccccaaccaa ggctaccgct tcgccgcggc gcacggaatc tccatttttc
     6781 ttgagaaaat ccgatatatg agcatatacc gtgtaatagc ggtcgccgtg gtcgataatc
     6841 aacattttgc catatccgga gaaacggtca gcaaagacaa ctttaccctt ttcaacagcc
     6901 cgaatctctt ccccaaccgg agcttcgatt tccaaacctt tacgatagac ctcggtcgaa
     6961 aattcatggt gtttggcttt acctaagccg ccgatcactt cgcctttgac tggccaatcg
     7021 agctttccac gcatagattc caaaccagca ccggccgaag cctccttgga tctggccgct
     7081 gagcgccgag agatttcctc gatcattctc tgcaatcgtc cggcagcttg ctctaaatcc
     7141 ttcaacgcgc gctggcgaga ttccttttcc tctctaatgc tggcgagcaa ctcacgtttt
     7201 ttttgcgcct ctttttgaac cgcttccttg acctgtgcta agtcgcgtct ctgatcgtca
     7261 acatcttgcc ttttccgggc cagttcttgc gcgagccttt cctgatgtat cgcctgttcg
     7321 tttaacgatt gtaccatctc ccggtcaaag gccaccgtgc gttgcaaata atactttcgc
     7381 ttgagtaaag agcttaacga tagatcgcca ttgaaaatga ctagtggact gccactacgc
     7441 tgccaacgat aaagcgcttc agcacgtcgc gccaaatagg ctttgcgctg ctccagcgat
     7501 tcgcgcattc gcaacgcctc cgcctctttt ttctgcagtt ccgacacgac gacttctagt
     7561 ctggcggtcg attgtttcag ttgcttattt tttcgttcaa gatcgctctc cacctgactc
     7621 agggattgga tcaccgaacc ttcctttttg ttcacctgag aaagaaccct tttttccgat
     7681 tcaattttct tcttaattcc ttccaaatct cggtcggcac tcgccgaata agctagcgcc
     7741 gctggcagac cggccataag acatacagcc acagtcgcta accgcgccac gttttaagaa
     7801 acctcctaag agagaagaga ctgccaaagc cgccgagcag caagccgata gcgactatca
     7861 ttgcgatgct agcaggatca agaaaacgca attgcatgtt ggacccgaat agacccattt
     7921 ggtcgaccat gtattgctgc aacaacaaat aacacaacca taaacagaat atagaaatgc
     7981 cggcaccaac gctcccttga agcatcccct caaccacaaa cggcgcccga atcaatcctt
     8041 ccgatgcacc cacgaactgc atgatttcaa tctcatcctt gcgtgccaac aaggccaaac
     8101 gaacggtact gccgacgata aaaaacgtgg cgatgaaaag aatgccacct agcgcccatt
     8161 tggcccattg gaccgctaac acgaacaggt tgagccggtc aacccattgt tgcggatact
     8221 ccacggccgc tattccgttg atcttgccga ggcgacccgc gagatcctcg atttgcagcg
     8281 ggtcacgata ggcaggcttc acggtgattt caaaggatgc gggcaacaca tcggggggca
     8341 ggccttctaa aacagcggac tgggcaccta aagcggtttg gaaatccatc caggcttgct
     8401 ccttggagat atgccgaaac gcttcaactt caggcatggc gcgaacgcga ctcataagcg
     8461 cttccaggcg atcgaggtcg acatcattat ccagataggt gtttatctga atctgatctc
     8521 cccagccgtt tagcaaatgc tgaagattct cttgaatgag gatgaaaaca ccgaaaacga
     8581 atagcgatgt tgccatcgtt cccgaagtaa gcacatggct ccacgccagc tcccataagc
     8641 tttgccgcac acgacgagcg acaaaggcca gatgcgacca actcaagacg cgtgcgcctc
     8701 cgtcactctc tcgagatcat caacaagtcg accctgacgc agggaaagaa ttcggctgcc
     8761 gtagcgtttg atcaaatcca gattgtggct agcgacaatg agggtggttc cagcttcgcg
     8821 cagtttgaga aatatccgca tgatttcctc ggcaaggccg ggatcgagat ttccagtcgg
     8881 ttcgtcagcc aagatcaatt tgggttcatt gatcaatgct cgtgcaatgg ccacgcgttg
     8941 ctgctcgccg gctgagagca tcagcggttt agcttgcggt ttttccagcc cgagttcctt
     9001 caggatgcga acagctttga tttgagcctc tcgtcgtgac acccccacca cttgagccgc
     9061 tagcgcgaca ttctccaaca ccgtgagccg cggcaacaat ctgaattctt gaaagacgag
     9121 tccgatccgc cgccttaggc gagcgactcc gcgggtgtgt aaacgcgtga cgttctgatg
     9181 atcaatcaga atctgcccct cagtcggttc ctcctccctg aacagcagct tgagtagcgt
     9241 cgtttttccg gcgccgctcg ggccggtgag aaaaacgaac tggcccgtct cgatacttag
     9301 ggtgatgttg accagagccg gtgaacctgg cggatacgtt ttggttacgt gagacaattc
     9361 gatcatcgag ggtagctgcg ggaggcaact agaagtttgc caaattccgc aaaaagcttg
     9421 acttggtata gggtggttgt tacagttccc aaaggcttga atttattaat atatctataa
     9481 gagatgtcgc tcatacttac aagcgcacgg agggcagcgt gacgcatcaa caaaaatcaa
     9541 ttgaaaaatt tcgcatagtt ctgatcaaac cctccaaata tgacgacgac ggttacgtca
     9601 ttcgcttttg gaaaggcgtc ctaccaagta ataccttgaa tgttcttcat ggcttgactg
     9661 aggatgtcat acagcgcggc gtgtttggag atcttccgat cgaggttacc acattcgacg
     9721 aaaccgcgga aaaagttccc ttgcacaaaa tcattcgctg gagcggcgaa ccgaacacgc
     9781 ggctgctagt ctgcatggtt ggcgtgcaaa ccaatcaatt tcctcgcgca cttgacatgg
     9841 caagggttct gcgcgccgag ggaatcacag tgatcatcgg cggctttcac accagtggaa
     9901 ccgtcaacat gttgggggaa caggaacccg acatgcaaga gctttttcgc gaaggcatct
     9961 gtgcggtgtc cggtgaagtt gaaggcaagt gggacatgat tctagcggat ttcctcgaag
    10021 gcaaactcaa accgctttac agcttcgcgc aagacttaca aagtttggtc gatatcgagg
    10081 attcgccgtt gccgaaaacg agctccaaaa caatggagca ttttgcctac ccgagctttg
    10141 gcaccgctga cacctcacgc ggttgtccat ttgcctgcag cttctgcacg attatcaatg
    10201 ttcaaggccg caagatgcgt gagcgaagcc cacaaagcat tgcggcgatg atgcgccgga
    10261 attatcgcga tcatggtgtc tctttttatt ttttcaccga tgataacttt gcccgaaaaa
    10321 aactctggcg cgaaacgttt gaagagatca ttaaacttcg agaagaaggc ctcgacgtga
    10381 cctttatgat gcaggttgac cttgcgagaa agccgaaaga tttcgtctca ctggcggcga
    10441 aagcgggctg cacccaagtc tttatcggca tggaaagcgt caatccgcag aatttggagt
    10501 ctgaaggcaa aaagcaaaac aaagttgaag agtatcgcga aatcatccaa gaatggcatc
    10561 aggtcggagt agtggttcac accggctaca tcatcggcct cccctgggat tcaaaagagc
    10621 aggtgccgaa tgacatccgc tttctcatcg acgaagtcgg ccccgatcaa gcttcgttct
    10681 tcatgctcac tcccctgccc ggatctcacg atcatcgaga aatgaaaaga cggggcgatt
    10741 ggatggaccc cgatttcaat aagcgcgact catttcatgc gaccatcaag cacccgctga
    10801 tgacagaaga agagtggacc gactgttatg agaaggcttg gagaacattt tacagcaaag
    10861 aaaatatgat cagggtcctt tcaaggtgga ccgataatcc gagaaactac tggaacctga
    10921 tgtcgatttt cttgtggtac aagaatgccg cactgattga gaaacaacac cccatggtcg
    10981 ccggtttttt ccggctcaag gatcgcaaag cccgccggcc cggttatgct atcgattcga
    11041 taccggttca tgcctggaag cgcagtaaag aaattacgca cctgcttgtc aattgggcca
    11101 agttcttgaa ggaaatcgaa gaagtctggc tgcaaacccg gaagaagagc gataccgagg
    11161 agcgctgggt ggagcagatc cagaaaatac aaaccgatat ttggcaagct ttacggattg
    11221 gtgaactgca aaaactttac agtaacgcaa gagaaacgct ttcgggccag gctagaacgc
    11281 tgctcgatcc cctggaagag ctttcgtcga gaattcttta caaccgcagc gatttgaatc
    11341 ggtttctcaa gcagtgggaa cacctacgaa acaggataca ggagatccgt tctatcgaaa
    11401 ctgatgcggc acgatcttgc ctggatgaaa tgcacaatct acagtccagc gtccgcctca
    11461 ctgatcgcgt gagcgagtgg caggaagctt acacacgatt acggggttca ttaccatcgg
    11521 gggctagctt acggctgatc aagttcgacg cgattaatta tcgagtggtg tattctcgtc
    11581 aggaactgca gcgaatttgg agccatacaa ttcacaatgc gcgccagatg aaactatggc
    11641 agatccggcc gtggcgcgtt acgcgggcca tgatccagga tttctcatta acgacttcct
    11701 tcgcggcaaa ctttaaagca ttcgccagaa gataaagaag ccgttccaga gacttgggtg
    11761 ttagtggatt tggacgtatc gctgcctgac cgattcgatc tgcgccttga ccatctccac
    11821 atcgtcggcg ccgggagcca atcgtaagta gccttccaga tcctcaagcg cctgcgcata
    11881 ccgctcaagg gttatgtaca aaagcccgcg gtcgcgaatt tcgctggcgg accgaggatc
    11941 tagtatcacc aactgatcga gcaccgacag agttttcaac ggttcgcctc tctccaggta
    12001 aatccatttt aagttattga gcatccggcg caaaatgtct cgtttcccta gagcggcgac
    12061 aaaaccggct tgatatgaca cctgacctcg atacagctgc tgcagcattc gctcaagatc
    12121 ttcccgagca cggatctcgc ccccgttgaa aacatcgatc aagatctcga cagcgtcgtc
    12181 ctcgtacttt acgagaaaat gacccgggaa gcctaccccc acgagcgata acccaacccg
    12241 tcgagccact tccatgtaga tcactgaaag gctaataggg atgcctttct tgcgcgtgat
    12301 aacatcgttc aagaaactgt ttttcggatc gtagtattca gaacggtttc cttgaaaacc
    12361 tagctctttg aagagaacgg tattaagtgc ggcgataatg cggtagggat ttttctcgtc
    12421 acccattcta agctcgactg cctgtcccaa gcgatcgagc tgagacaggc agtcgtcaat
    12481 tttaagatcc ggatattcct gcgtagcgat agctacagcc gcgtggccga gatcgatctc
    12541 atcttcgggg cgatcaacgg cctcgcggaa ggcaacgtac agatcgttgc tcacagccga
    12601 agactcctag cgcggctctg aaatcacgtc caccaaagca ggcaattgct tctccttgac
    12661 gtattttagc gcccgctcga gcgctggacg caattccgag gtccggcgga tcggcccttc
    12721 cccatgaacg ccaaaagact gggccatctt cgcataatcg acctcagggt cgtccacgtg
    12781 agttcctata cccgcgttct cgaccggtcg gttgcgaaac ttagccactt caatcccatg
    12841 ctcttcggag ttgtagaaag actgattatt gtgcagcacc atcagcaatg ggatcctgtg
    12901 tttggccgcg gtccagagac aactcgaagt catcagcaaa tcgccatccg actggatatc
    12961 gactgcgacc ttgccgcttc ctttcaaagc caatgctgcg cccatggctg cgctcgcccc
    13021 gtaaccaact ccggcacccc cgctggctcc aagatactgg ttcggtttgg tgaactccca
    13081 taaacggcgg gcccaaccat ttgaggttcc gttcaccagc acccaatcct ctcttttgat
    13141 cacctcaccc agatcatacg cgagaaacgc cgttgagatg ttttgcagcg aagccttggc
    13201 ttgcgcatcg gccaaccact tcgcgcgtcg actcttactt ttctcggcca gttctttctg
    13261 tctcgcctcg attaccgatt ttctcttcga atcgttgcct aaaagttccc gacagaggcg
    13321 agtcagttcc ggcacagcca ccgaagtatc cgcggcgagt ggaatatcgg tgggctgcaa
    13381 ggcctgataa tctccggccc atgagcgcac cagcatgtcg tgcatcgaaa tagtgatgat
    13441 ctttactgag ggcgaggtca catacacgca gtcgcgagtt tgcttgctga ccgtcgtaag
    13501 cgatccatac aagtcggcca cgtccagagc caaaatcaca tcggccttgg gcaggatatc
    13561 ttttgcacca tcggtcgcat ctaacgggtg ggtgg
//
LOCUS       DASUTG010000556         5028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1866624, whole genome shotgun sequence.
ACCESSION   DASUTG010000556 DASUTG010000000
VERSION     DASUTG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866624"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1074)
                     /locus_tag="VF089_12835"
     CDS             complement(97..1074)
                     /locus_tag="VF089_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174896.1"
                     /translation="MANKLLLLLAIVAMSLTASAAFGQLKKVRLAIPTVDIVTLPLKM
                     AQAKGFYQKEGLEVEIILIAGALGVRAVLGNSVEFSTASGSIVAAAARGVPVKLVSVI
                     TAKPTFDLISEPQIQTFQQLKGKQIGISTRGGTFEHITRLILERNGLDPDKDVTILAL
                     GRQDDLHLALKSGRISAAIYSPPRSLMLYRDGFYKLAISGDYLPHYPIGGIGVTDDLI
                     RKRRADIHAFVTATLSGIDYYRQNRSEAIKFIASELKMSDLSLAGQMFDWHRTVLADE
                     SRPSQAWINGVLDFTRKSLELSAPISADQVFDSASWIKLAEEFGKTGLD"
     gene            complement(1085..2695)
                     /locus_tag="VF089_12840"
     CDS             complement(1085..2695)
                     /locus_tag="VF089_12840"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6174897.1"
                     /translation="MSHYPILIAGGGPVGMTLAIELGWRGIRCLLIEQTDGTVEFPTT
                     NLANTRTCEHLRRWGIADHMRFDSGYPADYPRNYVFVTRMDGYEIARFDHPANGAPES
                     RSPHSPEGRLWISKPYFDPVLHAHANSLPQVEIRYQTSFESFEQDADAVLAEVLDKKT
                     GRKETISADYLVGCDGGRSNIRRALEIKYQGIFSQGMNVAVLFRSPLLQHIEFGPAVQ
                     YQIINSQINGAIAAVDGNELWRLNVRNIKQEDLEFFNAPEKLRYALGESIPFELLAVR
                     PWTGHCVVAETYQDRRVFLAGDAAHLNWPAGGFGMNTGIGDAVDIGWKLAAVLKGWGG
                     PRLLDSYTAERKPIAMINVNEAAEMRASFDSQTPFSPKMSDDSEEGKQLRERARAAIL
                     RTRAKEFQHDSAGIELGYRYENSPICVSDGTQPPALDHGLYVPSTWPGARAPHVWLRD
                     GRSILDLFGKGFTLLNLSGRERTSDLTEAARRFDLPLATITIEEPKVRDIYQRNLVLV
                     RPDGHVAWRDDSVPDNASTLLDQVRGAL"
     gene            complement(2722..3681)
                     /locus_tag="VF089_12845"
     CDS             complement(2722..3681)
                     /locus_tag="VF089_12845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174898.1"
                     /translation="MGIKPTVLLASILFISIASPVWAQLKKVRFSVSAASIAEVPFRI
                     AHVKGFYRDEGLDLEVIMIRGAVGMQALLGGSVDYTSSSGSTIAAAVRGIPVKLVFIA
                     SAKPQFDLVVQPQIRTIQDLKGKVVGISSRGGAVDLLMQLLLQKNGLVPNKDVTSIVV
                     GAQEETVLALRAGRIAAGLFTPPRPLILQREGFTRLAYTGDYMPTYPSGGIGATQEKI
                     KTNPAEVLAFVKGSVRGLHFARHNRGEAMKILSDYFHIKDQALAEAFFELYLSRLPAD
                     GGADEAWMKGAIDFTQKSLGDSGKDIPTDQVFDFSFVTKAARQ"
     gene            complement(3878..4873)
                     /locus_tag="VF089_12850"
     CDS             complement(3878..4873)
                     /locus_tag="VF089_12850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174899.1"
                     /translation="MPNQAIDIHHHYVPKQLIEETKRHGKALGVDITEDKDSHALSFA
                     GSKPHRLQPALMDVERRIEIMNEGQVALATLEANTNSLGYRLRGEQGEEWCKLYNDCM
                     NELVKQRPERFAGMATVPLQDPARAAKLLEHSIVDMKFRGAFIGTNVNGQYYNTKDFD
                     PFWAKAQELGVVIVMHPEHIAGAERMGAFGLNAVCGNPADSTLSLGYMLYSGLFDRFP
                     DLKLCALHGGGFLPYHLGRFDKEFVTGQNVRPAQSAKPPSAYLRNLYFDTLVYDLDTL
                     EYLRSKVGNDRLMVGTDYPYTLGDWLAVEKVQALPCSQSDKDAILQGNARRLLKL"
BASE COUNT         1106 a         1375 c         1378 g         1169 t
ORIGIN      
        1 ggctctgaga tcttttgagc agcctgctga ttgtttgaag attttctaat cgtcggaaaa
       61 tacaatcctg atcttcaatc attgaacggg ctgacgttaa tctagtccgg tcttgccaaa
      121 ttcttcagcg agctttatcc atgaagctga atcaaacacc tggtcggccg agatgggggc
      181 gcttagctcc aggctttttc tggtgaaatc gagtacgcca ttgatccacg cttggctagg
      241 acggctttca tcggcaagca cggtgcgatg ccaatcgaac atctgtccgg ctagggatag
      301 atcgctcatc ttgagttcgg aggcgataaa tttaatcgct tcacttcgat tctggcgata
      361 gtagtcgatg ccactaagag tggctgttac gaaagcatga atgtcggcgc gccgctttct
      421 tatcagatcg tcagtgacgc caatcccacc gatgggatag tgcggcaagt aatcgcccga
      481 aatcgcaagt ttgtaaaaac catcgcggta aagcatgagg ctacgcggcg gactgtagat
      541 cgctgccgaa attctaccgc ttttgagggc gagatgaaga tcatcctgcc tgccgagcgc
      601 cagaatagtg acgtccttgt ccgggtctaa tccattgcgt tcgaggatga gacgggtgat
      661 gtgctcgaac gtgccgccgc gggtggaaat gccgatttgc tttcctttaa gctgctgaaa
      721 cgtctgaatc tgcggctccg agatcagatc gaaggtcggt ttggccgtga tcacagaaac
      781 cagttttact ggaacacctc gtgccgctgc cgccacaatc gatccagagg cagtggagaa
      841 ttccaccgaa tttcccagca ctgcgcggac acccaaggcg ccggctatca ggatgatctc
      901 tacctccaat ccttcctttt gatagaagcc cttggcctgc gccatcttca agggcaaggt
      961 gacgatatcc acggttggga tcgccaggcg aacctttttg agctggccaa aggctgcgct
     1021 tgcagttaaa gacattgcaa caattgccag aagcaacaaa agtttgttcg ccattctctt
     1081 ctttctaaag cgcgccgcgc acctgatcga ggagcgtcga ggcgttatcc ggcacggaat
     1141 catcacgcca cgcaacgtga ccatcgggac gaaccaaaac caaattgcgc tggtaaatat
     1201 cgcggacctt cggttcctcg atcgtgatcg ttgcaagcgg caggtcaaac cgtctcgccg
     1261 cctctgtcag gtcgctcgtg cgctctctac cggacagatt cagtaatgtg aaacctttgc
     1321 cgaacaaatc gaggatcgag cggccgtccc gcagccatac atgcggcgcg cgcgctcccg
     1381 gccaggtcga tggcacatag agaccgtgat cgagcgccgg cggttgtgtg ccgtcagaaa
     1441 cgcaaattgg cgagttctcg taacggtagc ccagttcgat gccggccgaa tcatgctgaa
     1501 attccttggc ccgggttcgt agaattgccg cccgcgctct ttcacgaagt tgttttcctt
     1561 cttcgctgtc atcgctcatc ttgggcgaga agggtgtttg actatcgaag ctggcgcgca
     1621 tctcggcagc ttcgttgacg ttaatcatcg cgatgggctt gcgctccgct gtatagctgt
     1681 cgagcaaacg agggccgccc caacctttga gcactgccgc caatttccag ccgatatcga
     1741 cggcatcgcc gatgccggtg ttcatgccga agccgcccgc cggccagttg agatgggcgg
     1801 catcgccggc cagaaaaacc cgcctgtctt gatatgtttc agcgaccacg caatgaccgg
     1861 tccaggggcg cactgcaagc aattcgaaag gaatactctc gcctaaggca tagcggagtt
     1921 tttccggcgc attgaagaat tccaggtctt cctgcttgat gttgcgcacg ttcaatcgcc
     1981 acaactcgtt gccgtccacc gcagcgatgg cgccgttgat ttgagaattg ataatttgat
     2041 attgcacggc cggaccaaat tcgatgtgct gcaacagcgg cgaccgaaac aacacagcga
     2101 cgttcatccc ctgagagaaa atcccttggt attttatctc cagcgcccgc cggatattgc
     2161 tgcgcccgcc gtcacaaccg accagataat ccgcgctgat ggtttccttg cgtccggttt
     2221 tcttgtccaa gacttcggct aacaccgcat cggcatcttg ttcaaacgat tcgaaagaag
     2281 tttggtaacg aatctcgact tgcggcagcg agttcgcgtg cgcatgtaat accgggtcga
     2341 agtaaggttt tgaaatccac agtcttccct cgggactatg cggcgagcgc gactccgggg
     2401 cgccgttggc ggggtgatcg aagcgggcga tttcataacc gtccatgcgc gtgacaaaca
     2461 cataattgcg cggatagtcg gcgggatagc cggagtcaaa gcgcatgtga tcggcgatgc
     2521 cccagcgccg caagtgttcg cacgtgcgcg tgttggcgag attcgtggtg ggaaactcga
     2581 ccgttccgtc ggtttgctcg atgaggaggc agcggatacc gcgccagccc aattcgatcg
     2641 ctagcgtcat gccgacgggt ccgccaccgg cgataagtat gggataatgg ctcatcgcat
     2701 tgcgcctgct catcatgtct ttcactgccg cgccgctttg gtgacgaagc tgaaatcgaa
     2761 aacctgatcg gtcggaatat cttttccgct gtcgcccagg ctcttctgag taaaatcgat
     2821 cgcgcctttc atccaggctt cgtcggcgcc gccatcggct ggaagccggc tcaagtacag
     2881 ttcaaaaaaa gcttccgcca gcgcctggtc tttaatgtga aaataatcgc ttaaaatctt
     2941 catcgcttcg ccgcggttat gcctggcaaa gtgcagcccg cgcacgcttc ctttgacgaa
     3001 cgcaagcacc tcagccggat ttgttttgat tttctcctgg gtagcgccga ttccgccgct
     3061 cggataggtc ggcatgtaat ccccggtata agcgaggcga gtgaagccct cgcgctgcaa
     3121 gatcaatggc cgaggtggtg tgaacaggcc agcggcgata cggccggccc tgagggcgag
     3181 aaccgtttct tcctgggcgc cgacgacgat actggtgacg tctttgttgg gtacgagacc
     3241 gttcttctgc aacagcagtt gcatcaatag atcgaccgcc ccgccgcgtg aagagatgcc
     3301 gacgactttg ccttttagat cctgaatcgt tcgaatttgc ggttgtacca cgagatcgaa
     3361 ttgaggcttg gcggatgcaa tgaagacgag cttgacagga atcccgcgca cagcggcggc
     3421 aatggtcgag cccgatgaag acgtataatc cactgagccg ccgagcagcg cctgcatgcc
     3481 cacagcgccg cgaatcatga tcacctcgag gtcgagcccc tcgtcacgat agaagccttt
     3541 tacatgagcg atcctaaatg gcacctcggc gatggaggcg gcgctgacgg aaaagcgaac
     3601 ttttttgagc tgcgcccaaa ccggcgaagc gatggagatg aacagaatag aggccaacaa
     3661 aaccgtaggt ttgatcccca tggtggaacc tccccggcaa gtaaacacgc gctaaacaga
     3721 cctgtcaata ttgacaaccg tggcttgacc tagatttgaa ggttttaaaa ccactcggac
     3781 gaaatccttc tgggctcaat gtcattttgc tgcgaaaatg gacgttcgac ttccgaatca
     3841 ttgagccttt gaacttttca accgttgaac tactaattta gagtttcagc agccgccgcg
     3901 cgttgccctg caagatcgcg tctttgtcgg attgggaaca gggcaaggct tgcaccttct
     3961 ccaccgcgag ccagtcgcca agtgtatacg gataatccgt cccgaccatg aggcgatcgt
     4021 tgccgacttt ggatctcagg tattccaaag tatcgaggtc gtataccagc gtatcgaaat
     4081 agaggtttcg caaataagcg ctgggaggct tcgccgattg cgccgggcga acattttggc
     4141 cggtcacgaa ttccttatcg aagcgcccca gatggtaagg caagaagccg ccgccgtgga
     4201 gagcgcataa tttcaaatca ggaaaacgat cgaacaagcc gctgtacagc atgtagccaa
     4261 gcgagagcgt cgaatcggct ggattgccgc acacggcatt cagcccgaaa gcgcccatcc
     4321 gttcggcgcc ggcgatatgc tccggatgca tgacgatcac gacgccgagt tcctgcgctt
     4381 tggcccaaaa cgggtcgaag tctttggtgt tgtaatattg gccgttgacg ttggtgccga
     4441 tgaaggcgcc gcgaaatttc atatcaacga tggaatgctc cagcaacttt gccgcccggg
     4501 caggatcctg gagtggcact gtggccatgc cggcaaagcg ctcgggtcgt tgtttgacca
     4561 attcgttcat gcagtcgtta taaagcttgc accactcttc tccttgctcg ccccttagcc
     4621 ggtagccaag gctgttggta ttggcttcca gggttgctaa cgcgacctgg ccttcattca
     4681 tgatttcaat ccggcgttcg acgtccatca acgcgggctg caatctatgt ggtttgctgc
     4741 cggcaaaaga aagggcgtgg ctatccttgt cttcagttat gtcgacgccg agcgccttgc
     4801 cgtgtcgttt ggtttcctcg atcagctgtt tcggcacgta atgatgatga atatcgatag
     4861 cttggtttgg cattgtcttc gctttctcgt ctaacctgtc actttgatag gctcctggag
     4921 ttattcattt tcaaatacgc cgcgcttccc gattagacta aatatgcggg ccgatcagtg
     4981 gcagcgacaa tagatgggct cgatcgaaat tgtcaacggg tttaccgg
//
LOCUS       DASUTG010000557         4345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1420714, whole genome shotgun sequence.
ACCESSION   DASUTG010000557 DASUTG010000000
VERSION     DASUTG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420714"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF089_12855"
     CDS             complement(<1..297)
                     /locus_tag="VF089_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174900.1"
                     /translation="MRRMFSRWISAIAVLFMAVLAEQPAGAQEFYKDKTIRFIVGYSA
                     GGSFDLYTRVIARHFSKHVPGAPTTLVENMTGASGIIAANHLYKIAKPDGLTVGA"
     gene            complement(316..918)
                     /locus_tag="VF089_12860"
     CDS             complement(316..918)
                     /locus_tag="VF089_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6174901.1"
                     /translation="MIRYQCLELLAAKITDQLLVTSQSGQRIEWSHLSKHEGNLLVGM
                     MGCAIGVGMGLALALPHRKVIVLDSDGSVLLSLFNLATLGNLQPKNLIVYVFDNGVYS
                     GSRISYATATSGNTDLEAMAQGAGIKSARTIRDIEQFKKQGIDALAGNELCFFVCKVE
                     ESLMHREIPRPNTDLAENKYTFVRYLERTEKRTIPFIGRG"
     gene            complement(915..1421)
                     /locus_tag="VF089_12865"
     CDS             complement(915..1421)
                     /locus_tag="VF089_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6174902.1"
                     /translation="MTEELAQRIVQGLKDAGVNFVTYLPETRLSQIVPLIRDDPDMKL
                     IAAASEQEAVTIAAGATLGGKQAAVYMENTGIYVSSYSLLAVGKQLGVPILLVVGFLG
                     GIADQRNSFLYATIGNHTIPVLQGLGIEHMLLEDGDRLEVKIKDAVRGMNALRAPFAL
                     LFAGEFSL"
     gene            complement(1685..3937)
                     /locus_tag="VF089_12870"
     CDS             complement(1685..3937)
                     /locus_tag="VF089_12870"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6174903.1"
                     /translation="MKAVNSNNKIGTLIGTPTQRLEGPEKVSGQAIYATDVVLPGMLW
                     GKVLRSPISYGRITKIDTSRALALPGVRAIATGEDVKGLLIGRKIYDMPILADGLVRF
                     IGQKVAAVAADSEAIAEHAIELIDVDYEEMEALLDPLQAIEPSAPLLHPQVGGYRGLL
                     HPIQTPSNVFVDMNWKKGDVEAGFREADIVVEDVFTTKPVHQAYIEPHACVVQAQERD
                     ADIWACSKVPFALREQVATAFGKGLEQFTVHPCYIGGCFGGKGDFMDVPVCYVLSLKS
                     GRPVKMVMDYSEEFIAGNPRHAAIVRVKTGVNKDGRLIAHQMEYVFDSGAYGAFKPQG
                     YLVGPKEAAGPYRIPNVFIREQIVYTNKIPCGHMRAPGDPQGFFANESQMDLIARQLG
                     MDPVAFRQKNLLHDGELSPIGHVIPHIKSAELVESVVEKSGYRRPKRKRTGRGIGLAQ
                     WLPLGGECHALVTIDGEGAATVATAMLDQGAGTHTAMRLVAAEELKLPVEKIRVATLD
                     TSEVGPDTGIGASRGTRIFGHATRLAAAQAKEQLIEVASYKLGVPKEELFLSDGTVRS
                     AAGKSISYGEIAQASGEGLRGHGFYKNFESGPEAALCAQIAEVEVDGETGQVTVKQFT
                     TAHSTGTVLNPLMHQAQIDGGVVMGIGYALMEEVMIEDGKVMTTNFGDSKIPSIMDIP
                     PLKTIIQENPVGNGPYGAMSIGEPPVIPTAAAIANAVHDAVGVRIYDLPITAEKVLHA
                     LQAKKNKGES"
     gene            complement(4037..>4345)
                     /locus_tag="VF089_12875"
     CDS             complement(4037..>4345)
                     /locus_tag="VF089_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174904.1"
                     /translation="RDEVKKVTRALLRANRFILDNPKGAIKILAAWGRSKLDVAEEAY
                     QYNAKNYSRNLLVSRATLENVIESTRWNVETKGTVAVDDVFDFSIVREILKEMGETAK
                     "
BASE COUNT          890 a         1309 c         1163 g          983 t
ORIGIN      
        1 cgcgccgacg gtaagaccat ccggcttggc aatcttgtag agatggtttg ccgcaatgat
       61 gccgctggcg cccgtcatgt tctcgaccag agtggtcgga gcgccgggaa catgcttgga
      121 gaaatggcgc gcaatcaccc gcgtatagag atcgaaggaa ccgcccgccg aatagccgac
      181 gatgaagcgg atggtcttgt ccttatagaa ttcctgtgct ccagccggtt gctccgcaag
      241 cactgccata aatagcacag cgatcgcgct gatccatctt gaaaacatcc gtcgcatgcg
      301 tcctcctgcc agttgctagc cgcgcccgat gaaaggaatg gttctctttt ccgtacgttc
      361 cagatagcgc acaaaggtgt acttattttc ggcaagatcg gtgttcggtc tcgggatctc
      421 gcggtgcatc aagctttctt ccactttaca aacaaagaaa cagagctcat tgccggccag
      481 agcatcgatg ccttgctttt tgaactgttc gatatcgcga atcgtccgcg cgctcttgat
      541 gccggcgcct tgggccatcg cctccagatc ggtgtttccc gaagtggccg tggcatagct
      601 gatgcggctg ccgctgtaaa cgccgttatc aaagacataa acgatcaggt tcttaggctg
      661 gagattgccg agagtggcga gattgaacaa cgacagcaaa acgctgccgt cggagtcgag
      721 cacgattacc ttgcggtgcg gcagggccag agccaagccc atgccgacgc cgatcgcaca
      781 gcccatcatg cccaccaata aattgccttc atgtttcgac aggtgcgacc actcgatacg
      841 ctggccgctc tgggaggtca ccagcaattg atcggttatt ttcgccgcga gcagttccag
      901 gcattgatag cggatcataa cgaaaactcc ccggcaaaca gcagcgcaaa gggcgctcgc
      961 aaagcattca tgccgcgcac cgcgtctttg attttgacct ccagcctgtc gccgtcttcg
     1021 agcagcatgt gctcgatgcc aagtccctgc aggaccggaa tcgtatgatt gccgatcgtg
     1081 gcatagagaa aactgttgcg ctgatcggcg atgccaccca agaatcctac gaccagcagt
     1141 atcggcactc ccaattgctt gcccaccgca agcaggctgt agctggaaac gtagatgccg
     1201 gtattttcca tgtacacggc ggcttgtttg ccgccaagag tcgcgccggc ggcaatcgtc
     1261 accgcttcct gctcgcttgc cgctgcaatc aacttcatat ccggatcgtc tcgaatcagc
     1321 ggaacgatct ggctaagccg ggtttccggc aggtaggtga cgaagttgac tccagcgtct
     1381 ttgagaccct ggacgattcg ttgcgctagt tcttctgtca tagatggtgt gatgaattac
     1441 caaatgaaca tccccgtagc aaaccgtgga ggtatgaaga ggcactcaga ttttcccacc
     1501 gagttgtcac ccccgactgt tttaatcggg ggtccagtcc gattcgcctg gattcccgct
     1561 gaaagcatgc gggaatgacg gaccctagca gatccaccaa ctaccattgc gcatcaagct
     1621 gcgtggaatt gagatttaag ttacttcgtt cacagcggcc gactaaagtt tcacctttcc
     1681 actttcagct ttcacccttg tttttcttcg cctgcagcgc atgcagcacc ttctccgccg
     1741 tgatcggcaa atcatagatg cgcacgccga cagcgtcatg cacggcgttg gcaatggccg
     1801 ccgcggtcgg gataaccggc ggctcgccga tgctcatcgc gccatacggc ccattaccta
     1861 ccgggttctc ctgaataatt gtcttcagcg gcggtatatc catgatcgat ggaattttgc
     1921 tgtcaccgaa gttggtcgtc atcaccttac cgtcctcgat catgacctct tccataaggg
     1981 catagccgat gcccatgacc accccgccgt cgatctgggc ttgatgcatg agcgggttga
     2041 gcacggttcc ggtgctgtgc gcggtggtaa actgcttgac agttacctgg ccggtttcgc
     2101 catcgacctc gacctcggcg atctgagcgc aaagcgccgc ctcggggccg gattcgaagt
     2161 tcttataaaa gccgtgtccg cggagtccct cgccgctcgc ttgagcgatc tcgccatagc
     2221 tgatgctctt acccgccgcg ctccgcaccg tcccatcaga cagaaaaagc tcttccttgg
     2281 gaacgcccag cttgtagctc gcaacctcga tcaattgttc tttggcctgc gccgcagcaa
     2341 gtcgggttgc atgtccgaag attcgcgtgc cccggctggc gccaattccg gtatccgggc
     2401 cgacctcgct ggtatccaac gttgccactc ggatcttctc taccggcagc ttgagttctt
     2461 cggccgctac cagccgcatt gctgtgtggg ttcccgcccc ttgatcgagc atggcggtgg
     2521 caaccgtagc cgccccctcg ccatcgatcg tgaccagcgc gtgacactca ccgcccagcg
     2581 gcagccactg agctaagccg attccacgcc cggttcgttt tcgtttgggc cggcggtagc
     2641 ccgatttttc aacaacgctt tcgacgagct cggcgctttt gatatgcggg atgacgtggc
     2701 cgatcggcga aagctcaccg tcgtgcagca aattcttttg cctgaaagcc accggatcca
     2761 tgccgagctg ccgggcgata agatccatct gcgattcgtt ggcaaaaaac ccctggggat
     2821 ctcccggggc gcgcatatgt ccgcaaggaa ttttgttggt gtaaacgatc tgctcgcgga
     2881 taaaaacatt ggggatgcga taaggtccgg cggcctcttt ggggccaacc agataaccct
     2941 gcggcttgaa ggcgccgtag gcgccgctgt cgaatacata ctccatttga tgagcgatca
     3001 accggccgtc tttattgacc ccggtcttaa cccgcacgat tgccgcgtga cgcggatttc
     3061 cggcgatgaa ttcttccgaa taatccatca ccatcttcac cggccggccg ctcttcaggg
     3121 acaagacata gcagaccggc acgtccatga aatcgccctt gccgccgaaa cagccgccga
     3181 tataacaagg atgaaccgta aactgctcaa ggcctttgcc aaatgccgtg gcgacctgtt
     3241 cgcgcagcgc gaacggcact ttcgagcagg cccaaatatc ggcatcacgc tcctgtgctt
     3301 ggacaacaca ggcgtgcggc tcgatataag cctgatggac tggtttggtg gtaaaaacat
     3361 cttcaaccac gatgtcggct tcgcggaagc ccgcttcgac atcgcccttc ttccagttca
     3421 tgtcgacgaa gacgttactc ggcgtttgga tcggatgaag caggccgcga tagccgccca
     3481 cctgcggatg aagcagcggc gcgctcggct cgatcgcttg cagcgggtcg aggagcgctt
     3541 ccatctcttc ataatccaca tcgatcaatt ctatcgcgtg ttcggcaatc gcctcgctgt
     3601 cggcggccac tgccgccact ttctgaccga taaaccggac aaggccgtcg gcaaggatcg
     3661 gcatgtcgta gattttccgg ccgatcaaga gacctttaac gtcttcaccg gtggcgatag
     3721 cgcgcacccc gggtaatgcc aacgcccggc tcgtatcgat cttggtgatg cggccgtagg
     3781 aaatcgggct gcgcagaact ttaccccaga gcattcccgg caacaccacg tcggtggcat
     3841 aaatcgcctg gcccgacact ttttccgggc cttccagccg ctgtgtcgga gttccgatca
     3901 gcgtgccgat tttgttattc gaattgactg ctttcacggt aatcctgtgc cggcgcaatc
     3961 cagagcggct tgcacgcaga cgccgcgcag attcacggcc tcattgcctg accgattgag
     4021 tggaggagcc aggcagctat ttagctgtct cacccatttc ctttaagatc tcgcgcacga
     4081 tactaaagtc aaaaacgtcg tcgaccgcga cggtcccctt ggtttcaaca ttccagcgcg
     4141 tgctctctat cacattctca agcgttgccc gggacactag gagattacgg ctatagttct
     4201 tggcattgta ctgataggcc tcctcggcga catccagttt ggatctaccc caagcggcca
     4261 aaatcttgat cgcgcctttg gggttatcca gaatgaaacg gttggcccgc agcagggcgc
     4321 gggttacttt tttgacttcg tctcg
//
LOCUS       DASUTG010000558         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1567474, whole genome shotgun sequence.
ACCESSION   DASUTG010000558 DASUTG010000000
VERSION     DASUTG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567474"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF089_12880"
     CDS             complement(<1..560)
                     /locus_tag="VF089_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX6174905.1"
                     /translation="MPSDQEISAKTINVNVELAGPEWRLQTTMSVPTVPIGLKEMLPM
                     FFSFADGVIGTAANGIEQTGEKISCQKGCGACCRQLVPISETEARSIQDLVNRLPVLK
                     QSQIRDRFAEARRRLTESGLLEKLLHPETWAKDEGWSVAMSYFRLGIPCPFLEDEACS
                     IHAERPIKCREYLVISPAANCRQPSV"
     gene            complement(601..2163)
                     /locus_tag="VF089_12885"
     CDS             complement(601..2163)
                     /locus_tag="VF089_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174906.1"
                     /translation="MSSQVTRYDIVPYTGHALAQAHPDRLATIATLFGMKPEAVTRAR
                     VLELGCGDGLNLTSVALSLPEAECIGIDLAAAGIRKGQERVRALGLKNIELRQLDIMD
                     VGESLGRFDYIIAHGIYSWVPEAVREKLLAVCKQNLADHGVAYVSYNTYPGGHMRDMA
                     REMMRFHVSEFNNPQQRVTQARSLIKFIIDAASEPDLYRMVLQKELDRMTEFSDSSLF
                     HDDLSEYNRPVYFYEFAEHAGRHGLKYLCEAHFFEIQTGIFPARVVEVLNRISDSPIA
                     KEQYLDFLKCRRFRQTLLCHHERQLDDNPRPEKVNSFYIASPIRPVDADARLDSREQL
                     TFAGPKNSSLQTDNPLIKAAMSALGKAWPNAIHFHELFAQARGSCGIASAEDDARILG
                     ELLLRAYAGGIVEFSLVPPRLVTAPSEKPMVSALARLQSLEGTEVVNLRHFNLRIEDS
                     LTRWLLQLLDGSRDRNALVDELVALVKSGAAAVETENGTIPKGAEEIRCFLAAQVETK
                     LAELAEMGLLVA"
     gene            complement(3047..>3462)
                     /locus_tag="VF089_12890"
     CDS             complement(3047..>3462)
                     /locus_tag="VF089_12890"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HEX6174907.1"
                     /translation="EDPQLGKDREGQLNRLGAKSLRLMMYGMSNGVAQVISEITRRAP
                     IGAVNVLRIWSHGRGGGQTFTGGHGGEAYRVAHWTGISLGNIDALAPTLSELAPYFSP
                     HSRVELRGCEVASGADGEQLLLRLSAIWQVPVLAW"
BASE COUNT          794 a          976 c          889 g          803 t
ORIGIN      
        1 tccacactgg gctgccggca atttgccgcc ggcgagatca ccaaatactc ccggcatttt
       61 atcggacgtt ctgcatgaat cgagcaggct tcatcttcaa ggaagggaca tggaatcccc
      121 aaacgaaaat aggacatcgc aacggaccat ccctcatctt tggcccaagt ttcgggatga
      181 agcagcttct ccagtaaacc gctttcggtg agtcgccgac gtgcctcggc aaagcggtcg
      241 cgaatctgac tctgttttag aacaggtaaa cgatttacca gatcctgaat cgagcgcgct
      301 tccgtctcgg agatcggcac cagttgccgg cagcatgcgc cgcatccttt ctggcaagaa
      361 attttttctc cagtttgctc tatgccgttg gccgctgtac ctatgacacc atcggcaaag
      421 gaaaaaaaca tcggcagcat ttctttcagc ccgataggaa ccgtcggaac agacatagtt
      481 gtctggagcc gccattcggg tccggcaagc tcgacattga cgtttatcgt ctttgccgag
      541 atttcttgat cagagggcat ctaattattc cgtcatactg gtcagcgcca aactgaaact
      601 ctaagcaacc agcagaccca tttcggcgag ctccgcaagt tttgtttcca cttgggcagc
      661 aaggaagcac cggatttctt cggcaccttt ggggatcgtg ccgttctctg tttcaacggc
      721 tgcggctccg gattttacca gggccactaa ctcatctacc aatgcattgc gatcccggct
      781 gccgtctaac aactgcagca gccaccgggt taacgaatct tcgatccgga gattgaaatg
      841 acgcagattt acaacctcgg ttccctctag actctgcagg cgcgccagcg cgcttaccat
      901 tggtttttca cttggagcgg ttacgagtcg aggcggaacc agactgaatt cgacaatgcc
      961 gccggcgtac gctctcaata ataattcgcc aaggattcgt gcatcgtcct cagcgctggc
     1021 aatcccgcat gatcctcgcg cttgggcaaa cagctcgtga aaatggatcg cgttcggcca
     1081 ggctttgccc aaagccgaca tggctgcttt gatcaatgga ttatcggttt ggagcgatga
     1141 gttcttaggt ccggcaaacg ttaactgctc gcgcgagtcg agccgagcgt ccgcgtcaac
     1201 gggccggatg ggcgaggcaa tgtaaaatga gttcaccttc tccggccgcg gattatcgtc
     1261 caactgccgc tcatgatgac acaacaacgt ctggcgaaac cggcgacact tgaggaaatc
     1321 caagtattgc tctttggcga ttggactatc cgatatccgg tttagcactt ctactactct
     1381 ggcgggaaaa atacctgtct gtatctcgaa gaagtgcgcc tcacatagat actttaagcc
     1441 gtgccggccg gcgtgttcag caaactcata aaaataaacc ggccggttgt actcggataa
     1501 atcgtcatgg aaaagcgagc tgtcggaaaa ttctgtcatg cgatccagct ctttttgcag
     1561 aaccatgcga tagagatcgg gctcgctggc ggcatctatg atgaatttga tcagagagcg
     1621 agcctgagtg acgcgctgct ggggattgtt aaactcgctt acgtgaaagc gcatcatctc
     1681 gcgcgccata tccctcatgt ggccgcccgg ataggtattg tagctcacgt aagccacgcc
     1741 atgatcagcc aggttttgct tgcaaactgc gaggagcttt tcacgcaccg cctccggaac
     1801 ccaagaataa atgccgtggg caatgatata gtcgaaacgg cccagactct cgcccacgtc
     1861 catgatgtcg agttgccgaa gctcgatatt tttaagacca agagcccgca cgcgctcctg
     1921 ccctttgcgg attcctgccg cagcgagatc gatgccgata cattccgctt cgggcaagct
     1981 caatgccacc gaagtcagat tgagtccgtc accgcagccg agctcaagca cgcgcgcgcg
     2041 agtaactgcc tccggtttca ttccgaagag ggtcgctatc gttgcgagtc gatcagggtg
     2101 cgcttgtgcc agcgcatgcc ctgtatacgg cacgatgtca taccttgtta cttgactgct
     2161 catcgcttga tcggttgaca aactcgtagt ccaatcgtga ccggatgcga cggttcgccc
     2221 ctcgcggcgg gccaagtttc aggattccag caggaggcgc gtgccggaat aagatggcac
     2281 tgggaccatc gttgatgact gcatacgctt caaaggcggg aaacaagcaa atcattatat
     2341 cgatctcgac cctgcccgcc tccggagcta tttcgattgt tcctctgtat tcaatctcgc
     2401 cgattccatg accggcgcgc gctatcgaat taccatatct acagtccatc tggatcagga
     2461 tcgtcggcgt agtcgacgct aaagagaatt tcatttttga cgtgccaacg cgctgctttc
     2521 ttaatacgtc tccgctggca gggtcacatt gaatgaggtc gtcgcatcgg tgatggtggg
     2581 ttaaaaccgg cggcgaagcc gtgagatcaa ctttgaccca ggactgcatt cgtgacggcg
     2641 cgtgtagatc gttgctgaaa ttacgttggt cggtcagata cgaaggcgag gcgctcaccg
     2701 ccgtcaatcc tcgatactcg ccatcagaca acgttgtcgt agccccagct acgtcgcgcg
     2761 gaatgaaggc attcagccaa aatgtgatcg tgtgaacagg tctagctacc cgacacttta
     2821 cttgctgcaa ggtagatgac acggcctaag aaaagttcgt attgtactga tgagatgatt
     2881 atctacacac gttcagtagg attgtcatca aaagaagtcg atgcgtattt tcaagaatca
     2941 cgtatcgttt cagttgttaa ttccagtagg actcatgcac atcaaagcgc cactcggagt
     3001 cgccttaacg acagtcccat cccattccgc acggtactgc gtcgcactac caggccagca
     3061 cagggacctg ccagatcgct gatagccgca gtagcaactg ctcaccatcg gcgccgctgg
     3121 caacctcaca cccacgcaat tcaacccgac tgtggggcga aaagtatggt gccaattcgg
     3181 acaaagttgg tgcgagagca tcgatattcc caaggctaat accagtccag tgagcaacgc
     3241 ggtacgcttc gcctccgtgt ccgcccgtga acgtctggcc tccgccgcgg ccgtgggacc
     3301 atatcctcag aacattcaca gctccgatcg gtgcgcggcg ggtgatttcc gagatgactt
     3361 gggcaacccc gttgctcatt ccgtacatca tcaggcgcag cgacttggcg cctaaccgat
     3421 ttagctgccc ttcccgatct tttccaagct gtgggtcctc ca
//
LOCUS       DASUTG010000559         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2162894, whole genome shotgun sequence.
ACCESSION   DASUTG010000559 DASUTG010000000
VERSION     DASUTG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162894"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF089_12895"
     CDS             complement(<1..262)
                     /locus_tag="VF089_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6174908.1"
                     /translation="MFTGLIEDVGRVASLVLNKQSAVLTVESSLASGAIPLGASIAVN
                     GACLTVIEKDKRTVAFDVSPETLERTSLATIQPGTLLNLERSM"
     gene            complement(282..1412)
                     /gene="ribD"
                     /locus_tag="VF089_12900"
     CDS             complement(282..1412)
                     /gene="ribD"
                     /locus_tag="VF089_12900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6174909.1"
                     /translation="MLLLRQDQIDRRYMRMACRLAEKAAGRTSPNPMVGAVLVRKGRI
                     IGSGFHPFAGGDHAEIVALKEAGNKAEGATLYINLEPCNHYGRTPPCTRSLIAAGINE
                     VVVGMEDPNPLVSGKGLRALVRAGIKVRTGVLHEQCRVLNEAFTKLITRGVPFVTLKL
                     AASLDGKISTATGDARWISCEESRATVHLLRNRYDAVLVGAGTVIADNPQLTCRIAGG
                     RDPWRIILDARLRIPLDAEFLHQRGKDKNIIVTSDTAPAAKIGALETLGATVWRLPAR
                     EGAVRWQPLLRKLAKIGIASVLIEGGATTAAGALKEKVVDKMLLFYAPKLLGGDGRYM
                     IDSLGIEHIKQSVMLQNLKVQKSGADLLVSGYLQTSPVGRLR"
     gene            complement(1416..1919)
                     /gene="nrdR"
                     /locus_tag="VF089_12905"
     CDS             complement(1416..1919)
                     /gene="nrdR"
                     /locus_tag="VF089_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6174910.1"
                     /translation="MKCPFCHETENRVIDSRLSKDSNMIRRRRECSKCSRRFTTYERV
                     EEMMPLVVKKDGRRETYERIKIINGLKRACEKRPVSINTIEAIADRIERNLQERGEKE
                     ILSSVIGEATMRELHDTDEVAYVRFASVYRSFKDINEFMVELEELLKERKEISAARVS
                     ASRQKRN"
     gene            complement(2035..3300)
                     /gene="glyA"
                     /locus_tag="VF089_12910"
     CDS             complement(2035..3300)
                     /gene="glyA"
                     /locus_tag="VF089_12910"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6174911.1"
                     /translation="MSFLQQTDSEVFAAIQKETKRLEQNLELIASENVVSEAVMEAQG
                     CVMTNKYAEGYPGKRYYGGCEFVDEVESMAIARARELFGADHANVQPHSGSQANMTVY
                     LSVLKPGDCFLGMNLAHGGHLSMGSPVNFSGILYRVIPYGVSEKTETIDYDEVAKLAR
                     EHRPKLIIAGASAYPRIIDFAKFRQIADEVGALLMVDMAHIAGLIAAGLHPSPVPHAD
                     FVTTTTHKTLRGPRGGMVLCRSAYAKAIDSKVFPGMQGGPLMHVIAAKAVALKEALDP
                     TFKIYQQQIVNNAQALAKSLSSKGYRLTSGGTDNHLMLVDLRKSELTGKAAQETLDRA
                     HITVNRNAVPFDTRSPFITSGIRIGTPAVTSRGMKEEEMGLIGDLIARALDHVGDENV
                     LGEVANQVAALCKKFPVYPHRLPSNRPNQ"
     gene            complement(3300..>3794)
                     /gene="rpiB"
                     /locus_tag="VF089_12915"
     CDS             complement(3300..>3794)
                     /gene="rpiB"
                     /locus_tag="VF089_12915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6174912.1"
                     /translation="SLSITLMSAIGSDHGGVELKVFLAGVLRSKKIDVQDLGTHGSES
                     VDYPDFGQLVSQQVSQGDADRGILICTTGIGMSILANKYPNVRAALVQDRETARTSRE
                     HNDANVLVLAGATTDPVLAREILEIWINTPFAGGRHQRRLDKISEIERALGTLSSKSA
                     KDSE"
BASE COUNT          816 a         1069 c          966 g          943 t
ORIGIN      
        1 gcatcgaacg ttcgagattc agcagcgtgc cgggttggat agtagccaaa ctggtgcgct
       61 ccaatgtctc cggtgaaacg tcaaatgcca ctgtcctttt gtccttttca ataaccgtta
      121 agcaggcgcc gttgactgcg atgctggcac ccaatgggat tgcgccggac gccaaactgg
      181 attcgacagt caaaacggcc gattgcttgt ttagcactaa gctcgcgacg cgtccgacat
      241 cttcgataag accagtaaac atgcaaggcg aacctttgcc cttagcgcag tcgaccaacc
      301 gggctcgttt ggagatatcc tgaaactagc aagtcagcgc ccgatttctg aacctttaag
      361 ttttgcagca tgacagattg cttgatgtgt tcgattccca ggctatcaat catgtaacgc
      421 ccgtcaccgc ccagtagttt tggggcatag aaaagcagca ttttgtccac caccttctcc
      481 ttcaaagcgc ccgcggcggt agttgctcca ccttcaataa gcacgctggc aatgccgatt
      541 ttggccagtt ttctcagcag cggctgccac cgcaccgcgc cttcgcgtgc gggcaatcgc
      601 caaaccgttg cgcctaaagt ttccagagcg ccgattttag ctgcgggcgc ggtatcgctt
      661 gtcacaatga tgtttttatc cttgccgcgc tggtgtaaaa attcggcatc caacggaatt
      721 cgcaaacgcg catcaaggat gattctccaa ggatctcgcc ctcccgcgat tcggcaagtc
      781 agttgcggat tatcggcaat tacagtgccc gctcccacta acaccgcatc atatctattg
      841 cgcaacagat gcacggtggc acgagattct tcacagctaa tccaacgagc atctccggtc
      901 gccgttgaaa tctttccatc gagagaagcc gccagcttga gcgttacgaa cggcactccg
      961 cgagtaatca acttggtaaa tgcctcgttc agaactcgac attgttcgtg gagaacaccg
     1021 gtgcgaacct tgatgcccgc ccggacgagc gcgcgcagcc ctttgccgga caccagagga
     1081 ttagggtctt ccatgccgac caccacctcg ttgatacctg cagcgatcaa ggagcgggtg
     1141 cacggcggtg ttctaccgta atgattgcat ggttcgagat tgatatagag agtggctccc
     1201 tcggccttgt tgcccgcttc tttgagcgca acaatttcgg cgtggtcgcc gcccgcgaat
     1261 ggatgaaatc ccgatccgat tattctccct ttgcgtacca gcaccgcacc gaccatggga
     1321 ttggggctag tccttccggc tgctttctca gcaaggcggc aggccatccg catatagcgc
     1381 cggtcgatct ggtcttggcg caacagcagc accggctaat tccttttttg ccgcgacgcg
     1441 cttactcgag ctgcggatat ttcttttcgt tcctttagaa gctcttccag ctccaccatg
     1501 aactcattga tatctttgaa tgagcggtag actgaggcga accgcacgta agcaacctcg
     1561 tcggtgtcgt gaagttctcg catagtcgcc tcgccaatca cggagctcaa tatttctttt
     1621 tccccgcgtt cctgaaggtt tcgctcgata cggtcggcaa tggcttcaat cgtattgata
     1681 ctgaccggcc gcttctcaca ggctcgctta agcccattga ttattttgat cctctcgtac
     1741 gtctcgcgtc gaccatcttt cttgactact aacggcatca tctcttcgac ccgttcgtaa
     1801 gtggtaaatc gccgcgaaca cttggagcac tcgcgacgac gccggatcat attgctgtcc
     1861 ttgcttagac gtgaatcgat gacgcggttt tcggtctcat gacaaaaggg gcatttcatc
     1921 cgatacttcc ttcgagtaca gccaactcct tcactttacg gtaaaccagg caataggttg
     1981 tcgcaggacc gcagagtaaa tcctgtgagt tggcgggatt ggcgtaggtt ggatctactg
     2041 attaggtcga tttgatggga gccggtgcgg ataaaccgga aactttttgc atagcgcggc
     2101 cacctggtta gcaacctcgc ctaaaacgtt ttcatcgccc acgtgatcga gcgctctggc
     2161 gatcaaatca ccgatcagac ccatttcctc ctccttcatg ccacgactcg taacggcggg
     2221 cgtaccgatg cgaattccgc tggtgataaa tggagaccgg gtatcgaacg gcacggcgtt
     2281 gcggttaacc gtgatgtgcg ctcgatcaag ggtctcctgc gctgccttgc ctgtcagctc
     2341 ggatttccgc agatccacca gcatcagatg attgtcggtc ccccccgagg taaggcggta
     2401 gcccttggag ctgagagatt tcgccagtgc ttgagcgtta tttacaattt gttgttgata
     2461 aatcttgaac gttggatcca gcgcctcttt caacgccacg gccttggcgg cgattacgtg
     2521 catcaagggg ccgccttgca tgcccggaaa gactttgctg tcgattgctt tagcataagc
     2581 agagcggcat aagaccattc ctccgcgcgg tcctcgcagg gttttgtggg tcgtggtggt
     2641 aacaaaatcg gcatgcggga ccgggctcgg atgcagcccc gctgcaatca aaccggctat
     2701 atgcgccata tcgaccatta aaagcgcacc cacttcatct gcaatctgac gaaatttagc
     2761 aaagtctatg attcgcggat acgcgctagc gccggcgatg atcagttttg gccgatgttc
     2821 gcgggcgagt ttggccacct cgtcataatc gatggtttca gttttttctg aaaccccata
     2881 agggatgaca cggtacagga tgccggaaaa attgactggg ctgcccatgg acaaatggcc
     2941 tccatgcgcc agattcatcc ctaaaaagca atcgcccggt ttgagcacgg aaagatacac
     3001 ggtcatgtta gcttggctgc cggaatgagg ctggacgttt gcatggtctg cgccgaagag
     3061 ttctctggcc ctggctatgg ccatagattc aacctcatcg acaaattcac agccgccgta
     3121 gtaccgcttg cctggatagc cttccgcgta cttgttagtc atcacgcagc cctgagcttc
     3181 cattaccgct tcgctgacga cgttctcaga ggcgatcagc tccaaattct gttcgagcct
     3241 ttttgtttct ttttgaattg cggcaaaaac ttcgctgtca gtttgctgca gaaaagacat
     3301 tactcagaat ccttcgcgct cttgctactc agtgttccta aagctcgctc gatttcactt
     3361 atcttatcga gacggcgttg atgccggccg ccggcgaaag gcgtgttaat ccagatctcc
     3421 aaaatctcgc gcgcgaggac aggatcggtg gtggctcccg cgagaaccaa cacgtttgcg
     3481 tcattgtgct cgcgactagt gcgcgctgtt tcacgatcct gcaccaaggc cgcccgaacg
     3541 ttgggatact tgttggctaa gatggacatg ccgattcccg tggtgcagat tagtatcccc
     3601 cgatccgcat ctccttgtga gacttgctgt gacaccagct ggccaaagtc gggatagtcg
     3661 accgattcac tgccgtgtgt accgagatcc tgcacatcga tcttttttga gcgaagcacg
     3721 cccgctaaaa acactttgag ttctactccg ccgtggtcac tacctatagc actcatgaga
     3781 gttatcgaga gaga
//
LOCUS       DASUTG010000560         7845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2163589, whole genome shotgun sequence.
ACCESSION   DASUTG010000560 DASUTG010000000
VERSION     DASUTG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163589"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..571)
                     /locus_tag="VF089_12920"
     CDS             complement(221..571)
                     /locus_tag="VF089_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174913.1"
                     /translation="MKRTSALWVIIIALFLTASCDQIFDRDRGIFNWPGSARDLEGSW
                     YVNGDRDRRAEIVSAPGGLEARNERGQSSRLDITSGGSVRALDWEGGLRGTVRRDQIE
                     WQNGTTWTREARSR"
     gene            903..1898
                     /locus_tag="VF089_12925"
     CDS             903..1898
                     /locus_tag="VF089_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174914.1"
                     /translation="MNIRWLIMCTMIFSAISSNLFAQEKKLDNLRTGGGSASAAQMSI
                     WFAKEGNYYAKHGLNVELISIPGSSLALQAMLSGELPIIQAGGAGPIQVALSGTDTVI
                     VATIAKKFNWWIFSQPNISRMEDLKGKVFGTTRFGTQSDLASRIALRLHGLDPERDVT
                     MVQTGGPAETVTAMMTGKVHAAAISPPATLQAKRVKLKELMDLSKLDVEYHVNGLVTT
                     RRYLKSNEDVVRRFLKAYIEGAVRGQKDKAFAMKTMGKYFRTDDREVLEETYEMSIKN
                     GFSIPPYPAGISSLIQELEKQFPKARGAKPEDFTDSRLVRELDQDGFIRSVMAGR"
     gene            complement(2409..3392)
                     /gene="selD"
                     /locus_tag="VF089_12930"
                     /pseudo
     CDS             complement(2409..3392)
                     /gene="selD"
                     /locus_tag="VF089_12930"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
     gene            complement(3499..5430)
                     /gene="selB"
                     /locus_tag="VF089_12935"
     CDS             complement(3499..5430)
                     /gene="selB"
                     /locus_tag="VF089_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX6174915.1"
                     /translation="MPYILGTAGHIDHGKTSLIKALTGEDTDRLKEEKERGISIDLGF
                     AQLDLGDGNLAGVVDVPGHERFIKNMLAGAHGIDLVLFTVAADDGVMPQTEEHLDIVH
                     LLGIKRAIFVITKADLVSQARIAEVEEEIDILTLGTVLEDSPVIAVSSATGHGLSELK
                     QLISKSLQSIDKPAPSGYFRLPVDRAFVLQGHGVVVTGTARSGEIRTGDRVRCLPGDQ
                     IFRVRSLQVHGESVDLAGWGQRVALNLTGPDTAEVARGHVICHEKISLTSSRFDSYLE
                     VRPAARKGIKNHQSVRVHLGTAERLGKVIILGERDHIEPKQSAYCQITLAEPILALRG
                     DHFILRDETAQRTLAGGMVINPWARRHKRSEDLRNKLEALHRGDLTQLTEAFLEESDE
                     FALPLESIYQFLDCREELIRSSIDKMKNLRTVSAEGEKVYTTEPKWQRLKEQISKILK
                     DFHATHPLVPGMDMEELRGKLAYDLSPKVFRLVVDGLIGERLIAKDENLLRLAQHKLQ
                     LGGQDKLLMERIKKILAEHEMAPPELKQIEKDAAVPRAKLAEVLRLLERERSVVKVAT
                     DLYFLSSCVDKVRNLLYKHLTEQGEISAATFRDLLGSSRKYTIALLEYFDREGVTLRI
                     GDVRRLKSASATEKRGSAH"
     gene            complement(5665..7095)
                     /gene="selA"
                     /locus_tag="VF089_12940"
     CDS             complement(5665..7095)
                     /gene="selA"
                     /locus_tag="VF089_12940"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX6174916.1"
                     /translation="MLNSTMAYANLNSLMAEQATNLLRELPSVDRLLKHPRCEALLAR
                     YNRDYLIQKCRDALDQARIEIRQDKSIAVGAIKDEAIAARVEARIAAESQPALVRVVN
                     ATGTILHTNLGRALLSQAATDAVVAVAGSPVNLEYDLAAGKRGRREEAIEALLIELTG
                     AEAATVVNNNAAAVLLALNSLAEGREVIVSRGELIEIGGAFRIPEIMAKSNAILKEVG
                     STNRTHPQDYQRAINDKTALLLKVHTSNYKVVGFTAEVDLAQLVAIGREHNIPVMEDL
                     GSGALIDLSHYGLPKEPIVAERIGAGADVVTFSGDKILGGPQAGLLVGKKSLIGKMNK
                     NHLQRALRCGKLTLAALEGTLRRYRQSPDITQEIPTLRSFTRAVDEIGELGQELLPRL
                     QSALGDGYRLTLEESTSQIGSGALPTEELPTMVIAITHETLNANRIAEEFRRANPPII
                     GRIQDDRFMLDLRTIFDPEDLLPNFN"
     gene            7355..>7845
                     /locus_tag="VF089_12945"
     CDS             7355..>7845
                     /locus_tag="VF089_12945"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX6174917.1"
                     /translation="MRFAFRFRQGMKAAIPIWIAFVPSSLAWGIAAQAHGMSFAEIVL
                     MSAWVYSGPAQFAVLVPLAEGKSSLQVLVAGFLMNLRFLPMSAALAPYFHGVNRLTLL
                     LSSHVVSASSFIVPYLQFQKERQDVGDSGSTETTAGYGNLAFFLGVGSSSFSVWVIGS
                     AFG"
BASE COUNT         1642 a         2165 c         2186 g         1852 t
ORIGIN      
        1 gggcttgcct atccgatcag cggctatggc cggctgatgc gcgagtggcc ggcgtcgccg
       61 gatgagcagc ccgcgttggc tgctgatatc gcagctaata ctcggcttga gcgataactg
      121 gggcgcggca aagagtggcg cgaactctgc cgcaggaacg cctactgcat ccgtcactga
      181 ctaaacagct gagaaactcc ggcgtacggc cgttcggccg ttaccggctg cgcgcctccc
      241 gcgtccaggt ggtgccattc tgccactcga tctgatcgcg ccgaacagtg cctcggagac
      301 caccttccca gtctaaagca cggacactgc caccgctggt aatatccaac cggctgctct
      361 ggccacgctc gttgcgcgcc tcgagcccgc cgggcgctga gactatttcg gcgcgtcgat
      421 cccggtcacc gttaacgtac cagctgccct ccagatcccg cgccgatcct ggccagttaa
      481 aaatcccacg gtcccgatcg aaaatctggt cacaagaagc ggttaaaaat aaggcaatga
      541 tgattaccca aagcgcgctt gttctcttca cgataacctc ctggtctctg gccggtgcct
      601 cgtctatgac ctcttgagca gccggcaaat aaggttactt acgaacctgc aaggctcgca
      661 tgtctaagct gcaatgaagc tagtatatag gatttcagca aacgaaaaag tatagtgccg
      721 aaaagttaag tgattgccga ttttcaaacc agtgcaatcg gcccatcaag attttctcga
      781 aaccagccta gtaaatagaa cgggactgtg tgactggggc gctttctcga caagagcgac
      841 taaagctgtc ttatatagac tatagacgca tcatcgggcg ttttatgatg aggagaaaag
      901 gtatgaacat ccgttggctg ataatgtgca ctatgatttt ctctgcaatc tcatcgaact
      961 tgtttgctca ggagaagaag ctcgacaact tacgaaccgg cggcggctcg gcatccgcgg
     1021 cacagatgtc catttggttt gccaaagaag gaaactacta cgcaaagcat gggctcaatg
     1081 tcgaactcat tagtatcccc ggaagttcgc tggcgctgca ggcgatgctg tccggagagc
     1141 tgccgatcat ccaggccggc ggtgcgggcc caatccaggt cgccctctcc ggcacggata
     1201 cggtaattgt cgcgactata gcaaagaaat ttaactggtg gattttcagc cagcctaaca
     1261 tcagccgtat ggaggactta aaaggcaaag tatttggcac gacgcggttc ggcactcaat
     1321 cagacctggc ctcacgcatc gcattacgcc tccatggcct cgatccagag cgcgacgtca
     1381 caatggtgca aacgggaggg ccggctgaaa cggtgactgc catgatgacc ggcaaagtac
     1441 atgcggccgc catttccccg cccgcgacgt tgcaggccaa aagggtgaag ctcaaggaat
     1501 taatggacct gtcaaaactc gatgtcgagt atcacgtcaa cggtttggtg acaactcgga
     1561 ggtatttaaa aagcaacgag gatgtcgtgc gccgttttct caaagcttat atcgaaggcg
     1621 cggttcgcgg gcagaaggac aaggcctttg ccatgaagac catgggaaaa tatttccgca
     1681 ccgatgaccg cgaagtgctg gaagaaactt acgagatgag tatcaaaaac ggtttcagta
     1741 tcccgccgta cccggcgggc atctccagct tgatacaaga attggagaaa cagttcccaa
     1801 aggccagggg agcgaaaccc gaggatttca ccgacagccg cttagtgcgc gagttggatc
     1861 aagacggctt tatcagatcc gtgatggctg gacgctgatg acatctagtt cgttgtcagt
     1921 ggaatcgagc cgacgaccaa taaacccgaa ttctgcagta gagattgaca cagcgatcaa
     1981 gcggtaagcc ctccagagcg aatgcaatgg gccagcggct tgtagcggtc ctgcgcttcc
     2041 agtgctgcag cgagcttccg tgaaaattgc cagcccgtta ccaagcacca tgcacggtat
     2101 tgaaatcgtt cgctcctgcc ttcgctccta caggctcacc tcttgatcgg ggtataatat
     2161 caactaggaa ctttcgagtc cgggataaaa tgacgaatcc gaagatcagt tgccgagcga
     2221 ccaaacgata ttggacagtg tagaagtcta gcaggttcct gacttcggtg gcgggctgct
     2281 caaaaagttc cagaggtgag gcgctcgaag gatcgataag cggaggcgtg ctcttgcacg
     2341 ttgacgcgca atcggtcgag agcaacgaag cctatgggct ttttcagcag cccgcctaac
     2401 ggaatggttc agacgagccg tatccaggcc ttttgcagag acgtggcata gccgacttgc
     2461 gttgccgcgt cgacaccggc gctcttaagc tcttcaagaa gctgtgatgc atgccggttg
     2521 gcgacggcta tcagtagccc gcccgaggtt tgcgggtcca aggccacttc gatgaggccc
     2581 tcacggatcg attgttcgat gaatatttta tcctgcaaaa attcacggtt ccgtttgcag
     2641 ccgccggtga tgtagccttt ttcagctaac tgcgtggcat cttgcagaag cggcagcttg
     2701 gccgattcga tgaccaatgt cacaccactg ccgcaggcca tccccaaacc atggccaaga
     2761 atgccaaagc cggttacatc cgagcaggcg tgcaccgcgt atttgcgcat cacgctagac
     2821 gccgtggcgt tgagtgtggt cattgaccga accgcttgct cgacgctttc ttcggcggct
     2881 ttaccttttt ttagagccgt ggtgatgatg ccggtgccga gaggtttggt cagaatcagc
     2941 gcatcgcctt cttggacccc tacgttgcgg tagatcttat cgggatggat ggtcccggta
     3001 acggccaggc cgtatttgat ttcttcgtcg ataatgctgt gaccgccgat gattactgca
     3061 ccgctttcct tgaccttatc ggcgccgccc ttgagaatct ctcccaggat ctcgatatcc
     3121 attttacctt ttggaaaaca gacgaggttc aggcatgtct tgggttcgcc gcccatggca
     3181 tagacgtcgc tgagcgaatt ggtcgccgag atctgcccga aggtatacgg gtcatcgaca
     3241 atcggcgtga agaaatcgac tgtgttgacg atggcgaggt ccggtcgcag gcgaaatact
     3301 ccggcatcgt cggacgtttc cgtcccgacc agcatgtccg gatgattgaa ctttggcaga
     3361 tcgtgcaaaa cttgcacgag gtcggctggg ccgagcttag cggctcaacc cgcggctttg
     3421 accgactgcg tcaagcggac ggcttttttc tttccgatta tcatagatct gtgagtgtag
     3481 ttcgatcgca aactcaattc aatgcgccga gccacgcttt tccgttgccg aagcggattt
     3541 caggcgtcga acgtcgccga tcctcagagt cacaccttcc cgatcgaagt attccaatag
     3601 cgcaatggta tattttcggc ttgatcccag caggtcacgg aacgtggcag ccgatatttc
     3661 accctgctcg gtgaggtgct tgtaaagcag gtttctaacc ttatctacgc agctagacag
     3721 gaagtagaga tcggtagcca ctttgaccac cgaccgctcc ctttcgagca gccgcagcac
     3781 ctcggctaat ttcgcccgcg ggactgcggc gtccttttcg atttgtttca gctcgggcgg
     3841 agccatctcg tgttcggcaa ggattttctt gatgcgctcc atgagcaact tgtcttggcc
     3901 gccaagctga agcttgtgct gggccaaccg cagcaggttt tcgtctttgg cgatcagccg
     3961 ttcaccgatc agtccgtcga ccaccagccg gaagaccttg ggcgagagat cgtaggcgag
     4021 cttgccgcgc agctcttcca tatccatgcc cggaaccagc ggatgggttg cgtggaaatc
     4081 tttcaagatc ttggatatct gctctttgag acgctgccat tttggctcgg tggtgtagac
     4141 tttctcgcct tcggcgctga ccgtgcggag gtttttcatt ttatcgatcg agctgcgaat
     4201 cagttcttcg cggcaatcga gaaactggta aatggactcg agcggtaagg caaactcgtc
     4261 gctttcttcc aggaatgctt ccgtcaattg tgtgagatca cctcggtgta gcgcttcgag
     4321 cttgttccgc agatcttcgg aacgtttatg tcttctcgcc catgggttga tcaccatacc
     4381 gccggcgagc gtccgttgcg cggtttcatc acggagaatg aaatgatcgc cgcgcaaagc
     4441 caggatcggc tcggctaacg tgatctggca ataagccgat tgtttgggct cgatgtggtc
     4501 cctctcgcct aggataatga ctttcccaag ccgttccgcg gtccccagat gaaccctcac
     4561 gctctgatgg ttcttgatgc ccttgcgcgc cgccggccgg acctccagat acgaatcgaa
     4621 gcgactcgat gtcagcgaaa tcttttcgtg acagatcaca tgaccgcgcg cgacttcagc
     4681 ggtatcgggc cccgtgagat tcagcgcgac ccgttggccc cagccggcga gatcaacaga
     4741 ttcaccatgc acttgcaggc tgcgtacgcg aaaaatttga tctcccggta ggcaacgcac
     4801 ccggtcgccc gtcctgatct cgccgctgcg cgccgtgccg gttactacca cgccgtgacc
     4861 ctgcaacacg aaagcacggt ccaccgggag ccggaaatag ccgctcggcg ccggtttatc
     4921 gatagactgc aacgatttag aaatgagctg cttgagctcg ctgaggccgt ggccggtggc
     4981 tgaggaaacc gcgatgaccg gcgaatcttc caggacggtt ccaagagtca agatgtcgat
     5041 ctcctcttcg acttcggcaa tgcgcgcctg cgaaaccaga tccgctttgg tgatcacgaa
     5101 gatggctcgc ttgattccga gcaaatgaac gatgtcgagg tgttcttcgg tctgcggcat
     5161 gacgccatcg tcggcggcaa cagtaaagag caccagatcg atgccgtgcg cgcccgccag
     5221 catgttcttg atgaagcgct cgtggccggg gacgtcgacg acgccggcga gattgccgtc
     5281 gccgagatcg agttgggcaa aacccaagtc gatcgaaatg ccgcgttctt tttcttcctt
     5341 gagccggtcg gtgtcctcgc cggtcaaagc cttgatcagg ctggttttac cgtggtcgat
     5401 atgtccggcg gtgccgagta tgtagggcat cttctacaag cttttcttag gctgttgaaa
     5461 aaggctcatc tgcttcgttg cgcttgagcg atttcgcgtc aacgtacatg agtacgcttc
     5521 cgctcatcgc tctacgcgcg cctcgcattt gaaccttttt gaacagccta aaagacattg
     5581 ggttactgct tgaatacaat cgtggggaat attttcggct ttgactttcg tgcctatgtc
     5641 tttgcgtctt cagggcgatt tgtctcagtt aaaattcgga agcaaatcct cgggatcaaa
     5701 gatcgttcgc agatccagca taaagcgatc gtcttggatc ctgccgataa tcggcgggtt
     5761 agcgcggcgg aattcttcgg cgatccggtt agcgttaagc gtctcatggg tgatggcgat
     5821 gaccatggtg ggaagctctt cggtgggtag agcgccgcta ccgatctggg aagtcgattc
     5881 ttccaacgtt aagcggtagc catcgcccag cgcggactgc agcctgggca gtagctcttg
     5941 gcccaattcg ccgatttcgt cgacagcgcg ggtgaagctt cttaaggtgg ggatctcctg
     6001 ggtaatatcg ggcgattgcc ggtatcggcg caaggttcct tccaaagccg ccaatgtcaa
     6061 tttgccgcag cgcagagcgc gctgcagatg atttttgttc atcttgccga taagactttt
     6121 cttgccgacc aacagccctg cctgggggcc gccgagaatc ttatcgccgc tgaaagtgac
     6181 gacatcggcg ccggcgccga tgcgctcggc gacgatcggc tctttgggca atccgtagtg
     6241 gctgagatcg atcagcgcgc cgctgccgag atcttccatc accgggatat tgtgttctct
     6301 gccgatggcg acgagctgcg ccaaatcgac ttcggcggta aatccgacga ctttatagtt
     6361 gctggtatgt actttcagca gcagcgctgt tttgtcgttg atcgctcttt gatagtcttg
     6421 cggatgcgtg cgattggtgc tgccaacctc tttcaatatc gcgttgcttt tagccatgat
     6481 ctccggaatt cgaaacgcgc cgccaatttc aatcagctcg ccgcgcgaaa cgatgacttc
     6541 ccggccttcg gccaggctgt tcaaagcgag cagcaccgcg gcggcattgt tattgaccac
     6601 cgtcgctgct tcggctccag tcagctcaat caataacgcc tcgatggctt cttctcgccg
     6661 accgcgcttt cccgcggcta aatcgtattc cagattcacc ggagaaccgg ccacggcgac
     6721 aactgcatct gttgccgcct gggacaagag cgcccgccca agattggtat gaaggatggt
     6781 gcccgtggca ttaactactc gaaccagagc cggttggctc tccgcggcaa tcctcgcttc
     6841 aactcgcgca gcaatcgctt cgtctttgat tgcgcctacg gcaatgcttt tgtcttgcct
     6901 gatttcaatc ctggcttgat cgagcgcatc gcggcatttc tgaatgagat aatcgcggtt
     6961 gtatcgggcc agcagcgctt cgcaacgggg atgcttcagg agccggtcca ctgaggggag
     7021 ctcgcggagc aggttcgtcg cttgttcggc catgagggaa ttcaagttcg cataagccat
     7081 ggtcgaattc aacatgacta gcaggctgct gaaagagctc ataactcgtt gcgctcaaat
     7141 cgcctcgccg cgacgtattg taagtacgcc ttagctcgcg attttttgcg cgcctggcct
     7201 ctggatcttt ttgagtagcc tgcaaccaga gtttttcagc gacccggcgc ggtggaatga
     7261 atttcgcggc gcaaaagagt ttgaacgaga gtctggaagc gatcgatgtc tgggcttggt
     7321 tgatctttga accgcggtca gctaagcggt ttcaatgcgc ttcgcgttcc gttttcgtca
     7381 gggaatgaaa gccgcgattc ctatctggat cgccttcgtg ccgtcgtctc ttgcctgggg
     7441 cattgccgcg caagcacacg gaatgagctt cgcagagatc gtcctgatgt ccgcctgggt
     7501 ctactccggt ccggcgcaat tcgccgtttt agtaccgctc gcggaaggca agtcgtcact
     7561 gcaggttctg gtggcggggt ttctcatgaa cttgcgcttt ctgccgatga gcgcggctct
     7621 ggcgccatac tttcatggcg tcaaccgcct tactctgctg ctttcgtccc atgttgttag
     7681 cgccagcagt ttcatcgtgc cttacctgca gtttcaaaag gaacgacagg acgtcggcga
     7741 ttcgggatca accgagacca cagccgggta tggaaattta gcttttttcc tcggcgttgg
     7801 ctcgagcagt ttttcggttt gggttatcgg cagcgcattc ggata
//
LOCUS       DASUTG010000561         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1718319, whole genome shotgun sequence.
ACCESSION   DASUTG010000561 DASUTG010000000
VERSION     DASUTG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718319"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /gene="lysA"
                     /locus_tag="VF089_12950"
     CDS             <1..1101
                     /gene="lysA"
                     /locus_tag="VF089_12950"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6174918.1"
                     /translation="SVKANSNLTLLRTFAKEGSGFDIVSGGELFRALKAGGDPKKIVF
                     SGVGKKKDEIEYALNSEILMFNVESEDELIALNEIARAIGKKAPISIRVNPDVDPQTH
                     PYISTGMKKAKFGVEIKKSLETYKKAATLPNVEVVGVDCHIGSQLTSLSPFVDALARV
                     REYLDRVLVGSLEKEGAQIRYLDLGGGLGIHYNDETPPQPEEYARAIMEGLEGLDLTL
                     ILEPGRVIVGNAGILVTEVQYVKENEAKKFVIVDGGMNDLIRPALYGSYQAIRPVVET
                     KAEKIVADVVGPICESGDFLAKDREITRPQRGDFLAVMSAGAYGFTMASNYNSHPRAP
                     EILVDGDKYYVVRARESFEDLINGEAIPAALQ"
     gene            1332..2147
                     /gene="dapF"
                     /locus_tag="VF089_12955"
     CDS             1332..2147
                     /gene="dapF"
                     /locus_tag="VF089_12955"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX6174919.1"
                     /translation="MPFTKMHGCGNDFIFIDCLKNDIPDLGAIAGRLCDRRFGIGADQ
                     LLTVHPSKIADFKMEIYNADGSQVEMCGNGIRCFAKYVYEHGLTQNKELEVETLAGII
                     RPRIVGNLVEVDMGEPILDGRKIPVDADGQIVNRPLVVDGRTYTVTCVSMGNPHCVLY
                     LDDIESLDLEKIGPDFEHHPFFPKRVNTEFIKVLSPSEVNMRVWERGAGETWACGTGA
                     SAVAVAGVLGGKSERKLRVHLKGGDLFIEWRDNNRVYMTGAAEEVFQGTVKVN"
     gene            2159..3034
                     /gene="dapA"
                     /locus_tag="VF089_12960"
     CDS             2159..3034
                     /gene="dapA"
                     /locus_tag="VF089_12960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6174920.1"
                     /translation="MFTGSMVALITPFEGGKVDWQSLERLIEFHIKSGTHGIVPCGTT
                     GESATLSHEEHDEVIKAVIRAVNRRLPVIAGTGSNSTAEAVRLTRAAEKSGADGALMI
                     SPYYNRPTQEGIYQHYKTVASEVGIPIIVYNIPGRTGSKIEPETLARLAEIKNIAGVK
                     EATGSVDQAIDVIRLCGDNLAVYSGEDTLTFSLMALGGKGVISTVANVAPREMSQLTE
                     ACLNGEWEKGRDLQFKLTPLIRSLFIETNPIPVKTAAALLGKCTAELRLPLTPMADGN
                     VQKLKQAMIEFGLIH"
     gene            3072..3881
                     /gene="dapB"
                     /locus_tag="VF089_12965"
     CDS             3072..3881
                     /gene="dapB"
                     /locus_tag="VF089_12965"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6174921.1"
                     /translation="MAKNLNLVVCGAGGRMGTAVVRAVAQAAGMALVAAIDKPGSARL
                     GKDAGEISGAGSLGISLSDKIEPYLSPGTVIIDFTRPEASLDYLRAAAKKAVPIVIAT
                     TGFDPRQTTEIKRLSRRTPTLLSANTSLGVNVLVSLLGKAAKMLGDDYDVEIVEAHHR
                     FKKDAPSGTALALGRAVAGALNRNLDHVGIHGRRGIIGERSKKEIALLSVRAGDIVGE
                     HTVIFGGIGERLEFIHRAHNRDTFAQGAIRAAQWLADQKPGLYGMQDVLGL"
BASE COUNT         1025 a         1170 c         1208 g          945 t
ORIGIN      
        1 tcggttaagg ccaattcaaa tctgaccctg ctcaggactt tcgccaagga aggcagcggc
       61 ttcgatattg tttccggcgg cgagctgttc cgagcattaa aggctggcgg cgatccgaaa
      121 aaaatcgtct tctccggtgt cggcaagaaa aaagatgaaa tcgagtacgc tctgaattcg
      181 gaaattttga tgttcaacgt cgaatccgaa gacgaactta tcgctttgaa cgaaatcgcc
      241 cgcgccatcg gcaagaaagc gccgatcagt atacgcgtca acccggacgt cgatccgcaa
      301 actcatccgt acatctcgac gggtatgaag aaagcgaagt tcggcgtcga aatcaaaaaa
      361 tccttggaga cctacaagaa agccgcaaca ttgcccaacg tcgaggttgt cggggtcgat
      421 tgtcacattg gctcgcaact aacgtcgctt tcgccatttg tggatgcttt ggccagggtc
      481 cgggagtatc tcgatcgcgt gctggtggga tcgctggaaa aagaaggcgc gcagattcgt
      541 tatctggact tgggcggggg gcttggaatc cattataatg atgaaactcc gccgcagccc
      601 gaggaatatg ccagggcgat catggaaggc ctggagggac tcgaccttac attgatactg
      661 gagcccgggc gcgtgatcgt cggcaatgcc gggattctcg tcacggaagt gcaatacgtc
      721 aaagagaatg aagctaagaa gttcgtcatc gtcgacggtg gcatgaacga tcttatccgt
      781 ccggcgcttt atggctccta tcaggcgatc cgtccagtag tggaaacgaa agccgagaag
      841 atcgtcgctg atgtcgtcgg tccgatttgc gagagcggcg atttcctcgc caaggatcgc
      901 gagatcacgc gtccgcaacg cggcgatttc ttggccgtca tgagcgccgg cgcctatggc
      961 tttactatgg catcgaacta caattctcat cctagagctc ctgagattct cgtggacggc
     1021 gacaaatact acgttgtccg tgcacgcgag tcttttgaag atttgatcaa tggcgaggca
     1081 ataccggcgg cgctacaatg agaacccgtt caaacgttcc atagttcaca ggatcaacgt
     1141 tgggaatatg gacgctttaa tccagcggct tgcaaaaatt tcttccaggc tgctcaaaag
     1201 gatctcagag gcgagacgcg cgaagttcga tgagctcagg cgtacgcttc cgtacgttga
     1261 agcgaatcga gcgagtgcaa cgaagcatat gagtcttttt cagcagcctg caagcttcga
     1321 gtgagatgga aattccgttc accaagatgc atggctgcgg caatgatttt atttttatcg
     1381 attgcctaaa aaacgatatt cctgatctgg gcgccatcgc cggcaggctc tgcgatcgcc
     1441 gtttcggcat cggcgctgat cagttgttga ccgtgcatcc atctaagatc gccgacttca
     1501 agatggagat ttacaacgcc gacggcagtc aggtggaaat gtgcggcaac ggcattcggt
     1561 gtttcgccaa gtacgtttat gagcatggtt tgacacagaa caaggaattg gaagtcgaaa
     1621 cattggccgg tatcattcgc cctcggatcg tcggcaactt ggtcgaggtc gatatgggcg
     1681 agccaattct cgacggacgc aagattcccg ttgatgccga tggtcagatt gtaaatcgac
     1741 cgctggtcgt cgatggccgg acttacacag tgacgtgcgt ctcgatgggc aatccgcact
     1801 gcgtgctcta cctcgacgac atcgaatctc ttgatttgga aaaaatcggg cctgacttcg
     1861 agcatcatcc tttctttccc aaacgagtaa acacggaatt catcaaggtg ctaagcccta
     1921 gcgaggtgaa catgcgagtc tgggagcgcg gcgcaggcga gacctgggct tgtggcaccg
     1981 gcgcctcagc agtcgcagtg gccggtgtgc tcggcggtaa gagcgagcga aagctaagag
     2041 tgcatcttaa gggcggcgac ctgtttatcg agtggcgtga caataaccgt gtttatatga
     2101 ccggcgccgc cgaagaggtt ttccaaggga ccgtcaaagt taattaggag cagttttgat
     2161 gttcaccggt tctatggtcg ccttgatcac acccttcgaa ggcggcaagg tcgactggca
     2221 aagccttgaa cggctgatcg aatttcacat caagagcggc acccatggca tcgtgccgtg
     2281 cggcaccacc ggcgagtcgg cgaccttgag ccatgaagaa cacgacgagg tcatcaaagc
     2341 cgtcatcaga gccgttaacc ggcgactgcc ggtgatcgcg ggaaccggtt ctaattcaac
     2401 ggccgaagcc gtgcgcttaa cacgcgcagc cgaaaagtca ggcgccgacg gcgcgctgat
     2461 gatttctccc tattacaatc gcccgaccca ggaaggaatc taccaacact ataagacagt
     2521 tgcctccgaa gtcggcattc cgatcatcgt atataatatt cccgggcgga cggggtcgaa
     2581 aattgagccg gagacgctgg cccgcttggc cgaaatcaag aacatcgccg gggttaaaga
     2641 agcgactggc tcagtggatc aagccatcga cgttatccgg ctttgcggcg acaatctcgc
     2701 cgtttattcc ggcgaagata cgctgacgtt ttctctcatg gcgttgggcg gcaaaggtgt
     2761 gatttcgacg gttgccaatg tcgcacccag agaaatgtca cagctgaccg aagcttgcct
     2821 taacggtgag tgggagaaag ggcgcgattt gcagttcaag ctgactccgt tgatccgcag
     2881 cctttttatc gaaaccaacc ccataccggt gaaaaccgcc gccgcgcttt tgggtaaatg
     2941 cactgccgaa ctgcgcctgc cgttgacccc gatggccgac ggcaatgtcc agaaactcaa
     3001 acaggcgatg atcgaattcg gcttgatcca ttaaatcgcc ggccgcgcct cttcgacttc
     3061 gttcgacttc aatggcaaaa aatctcaacc tcgtcgtctg cggcgcgggc ggccgtatgg
     3121 gcacagcggt ggtgcgcgca gtcgctcaag ccgcgggaat ggcattggtt gctgccatcg
     3181 acaaacccgg cagcgcgagg ctcggaaaag acgccggcga aatttccggc gcgggaagtc
     3241 ttggtatcag tctaagcgat aagatcgagc cttacctcag ccccggaacc gtcatcatcg
     3301 atttcactcg cccggaagct tcattggatt atctcagggc agcagcgaaa aaagccgtgc
     3361 ccatcgtcat cgccacgaca ggatttgatc cgcggcaaac caccgagatc aaacgcttgt
     3421 cgcgccggac gcctactctc ttgtcggcca ataccagttt gggcgttaac gtgcttgtga
     3481 gccttctagg caaggccgcg aaaatgctcg gcgacgatta cgacgtcgaa atcgtcgagg
     3541 cgcaccaccg gttcaagaaa gacgcgccca gcggtaccgc tctggcgctg ggccgcgccg
     3601 tcgctggagc gctgaaccgc aatttggacc acgttggcat tcatgggcgc agaggcatca
     3661 tcggagaacg atcaaaaaaa gagatcgctc ttctctcagt ccgcgccggc gacatcgtcg
     3721 gcgagcatac ggtgattttc ggtggcattg gcgagcgtct cgagttcatc caccgcgccc
     3781 acaaccgtga taccttcgcc cagggcgcta ttcgagcggc ccaatggctt gccgaccaga
     3841 aaccgggtct ctacggtatg caggacgtgc tgggattgta gctacaagtt ctcacctctc
     3901 attcgcattg ctgagccgat cagcatcaat cctgaaacag cggcttcgta tctcctagac
     3961 aaaagacaaa accgccgaac acgcgcttcc gtcacgatgc tatagggctc cgagcagtga
     4021 cgaagaatgc agtaggtggt ggagaacttg tcagtttcgg cgcaagcttg cgcaaattac
     4081 attgaagagt accgcgatga tacattaagt actggttaaa atcaggtcag tgcgtttaaa
     4141 atgatcttta cgtacccggc actccaattc gcatgcccaa tcataccttc gatcgtcctc
     4201 cgccacccat tacaatagac acgaaggagt tggccgctcc gtctaatagc ggcgctgtca
     4261 tagcgcgatc acagacttta ccaagcgcat tgcccctggt tgtttttctt gcgctagctc
     4321 tgtcctgctt ggcgtttttc tcagcccc
//
LOCUS       DASUTG010000562         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_88808, whole genome shotgun sequence.
ACCESSION   DASUTG010000562 DASUTG010000000
VERSION     DASUTG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88808"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..858
                     /locus_tag="VF089_12970"
     CDS             256..858
                     /locus_tag="VF089_12970"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174922.1"
                     /translation="MQTVYVDMDDVLCQTAQHFLTILERDFGKRFTFEQLTDFDVGEA
                     CEITAVEREELYRIVHQGEELLNIPAIPGAIDVLEQWSAAGYEVAIVTGRPPDTYEPS
                     VQWLKKHRVAHDSILIVDKYGRFVPDGSRCISLEALMNHSFCWAVEDSPTMAMYLASQ
                     MKIPVALLDRPWNRKGTNHDLVSRHSDWIEIARALPGKNR"
     gene            complement(926..2368)
                     /locus_tag="VF089_12975"
     CDS             complement(926..2368)
                     /locus_tag="VF089_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX6174923.1"
                     /translation="MSFIKFASKSTATWTVMILAGCSVGPNYKSPEIAVPASWNEAQQ
                     SGFHTGPGPTLTDWWTEFNDPLLDTLVERAVKSNLDIRLAEARIRESRALRAATAADA
                     WPGLEAAGTYRRSRSSENAFVSGGGGDAFLGGNGAGDLFRTGFDASWEIDIFGGVRRR
                     VEAADANLAASVEERRNALVTLLGDVARNYIEVRGFQRRLTVARSNLRAQQETLDLTR
                     VRFEAGLSSDFEVAQAEGQVNTTAAQIPALQSALKGSVHRLDVLLGQQPGALWSELSN
                     EAPVPALPPQAHVGLPAELLRRRPDIRRAERILAAATAQVGAATADLFPRFSLTGAFG
                     FQSINAGDLISSPSRFWSIGPTVLWPVFDAGRIRANIAVRDAQQEQALTLYEKTVLTA
                     FEDVENSLVNYAQEQTRYRALIDAVAANRRAVQMANELYTRGLIDFLNVLETQRSLYA
                     SESELAQSETIMASNLVALYKALGGGWETD"
BASE COUNT          547 a          718 c          659 g          528 t
ORIGIN      
        1 gcgcgaatgc cattgacgtt ccaagtcgca attttcaacg aaggcataat tttctccagt
       61 gcggaagtat agaaacaggc cgctggaact tcaatcacca aacaaaccag atcttaggac
      121 gttaacattg gcggcaagaa gttgggcaac ctagcggatg accgatacaa ggttgattgt
      181 atcagaagat tacttgattc tagcaagata ccgggtcgtg ccgatcgcaa ttggacgcgg
      241 ggaaggatgg actgcatgca aaccgtttac gtcgacatgg atgatgtatt gtgccagacg
      301 gcgcagcact ttctgacgat tctcgaacgg gatttcggga aacgatttac ctttgagcaa
      361 ctcaccgact tcgatgtcgg cgaggcttgc gagatcacgg cagtggaaag agaggagttg
      421 taccggatcg tgcatcaggg tgaggagctg ttaaacatcc cagcaatccc tggagccatc
      481 gacgttttgg agcaatggag cgctgccggc tacgaggttg ccattgttac aggccgccca
      541 ccggacacct acgagccttc cgtgcagtgg ctgaagaaac accgggtggc ccatgacagc
      601 atattgatcg ttgataaata tggacggttc gtgccggacg gcagccgctg catctctctc
      661 gaagcgctga tgaatcacag cttctgttgg gccgtggaag actcgccgac catggccatg
      721 tacctggcat ctcagatgaa gattcccgtt gctctgctgg atcgcccgtg gaaccgaaaa
      781 ggtacaaatc atgatctggt aagcaggcac agtgattgga tcgaaattgc gcgggcactg
      841 ccgggaaaga accggtgatg gtgcgctcaa cccgggcgat acaaaaatct tccgttcatc
      901 ttccgttctc cctgatcatt ctctcctaat ccgtctccca tccgcctccc agcgctttat
      961 acaacgccac cagattcgaa gccatgatgg tttcgctttg ggcgagctcg ctttccgacg
     1021 catacagcga gcgttgtgtt tccaagacgt ttagaaaatc gatcagacct ctggtgtaaa
     1081 gctcgttggc catctgcaca gcacggcggt tggcggccac cgcgtcaatc aaagcgcgat
     1141 aacgggtttg ttcctgggcg tagttcacca aagagttctc cacatcttcg aaggcggtca
     1201 atactgtttt ctcatacaag gttagcgctt gctcctgctg cgcatcgcgc accgcaatat
     1261 tggcgcgaat ccgcccggca tcaaagaccg gccacaaaac agttggtcca atcgaccaaa
     1321 agcggctcgg gctgctgatc aaatcgccgg cattgatgct ctggaacccg aaagccccgg
     1381 tcaatgaaaa tcgcggaaac agatccgccg tggcagcgcc cacttgcgcg gtcgccgcag
     1441 ctaagattcg ctcggcgcgc cgtatatctg gccgccgcct gaggagctct gcaggcaagc
     1501 cgacatgcgc ctgcggcggc aatgccggga cgggcgcttc gttgctgagt tccgaccaga
     1561 gtgcgcccgg ttgttggccg agaagcacat cgagacgatg cacgctcccc ttaagcgcgc
     1621 tttggagcgc tgggatctgc gccgccgtcg tgttcacctg gccttcggcc tgagccacct
     1681 caaaatcgct cgacagtccc gcttcaaacc gcactcgagt gagatccaag gtttcttgtt
     1741 gtgcgcgaag atttgagcgc gccaccgtta gccggcgttg aaagccacgc acctcgatgt
     1801 aatttctggc gacatcaccg agcaaggtca ccaacgcatt gcgccgctct tcgaccgagg
     1861 ccgcaagatt ggcatcggcc gcttccaccc ttcgccgcac cccaccaaaa atgtcgatct
     1921 cccaactagc gtcgaagccg gttctaaaga gatcgcctgc gccattgccg cccaaaaagg
     1981 cgtcgccgcc tccgccggaa acaaacgcgt tctcacttga tcggcttcgc ctgtaagtac
     2041 ccgcagcttc caaccccggc cacgcatccg ctgccgtggc tgccctgagc gcccgcgact
     2101 ctcgaatgcg tgcctcagct aagcggatat ccaggttcga tttcacggct cgctccacca
     2161 gcgtatcgag caatggatcg ttaaactccg tccaccaatc cgtcagcgtc gggccaggcc
     2221 cagtatggaa gccggattgc tgtgcttcat tccagctcgc cgggactgcg atctccggcg
     2281 atttataatt tgggccgacg gagcatcccg ccagaatcat cactgtccaa gtggcagtag
     2341 atttacttgc gaatttaatg aagctcatag ggcggtcctt cttttgagtc attattcatc
     2401 tctctgttac gataaccgat ggcgaaacat ccatgagtct gccgacagcg tg
//
LOCUS       DASUTG010000563         8787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2014915, whole genome shotgun sequence.
ACCESSION   DASUTG010000563 DASUTG010000000
VERSION     DASUTG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014915"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VF089_12980"
     CDS             <1..733
                     /locus_tag="VF089_12980"
                     /inference="COORDINATES: protein motif:HMM:NF014954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX6174924.1"
                     /translation="DTALWDRVQGLPEEEILTWYEKAVDELLMLQIRGTAARDEACVA
                     FQQRFDFRLYMWEFDHFLEYGLEKRPGTHVHATAIETLRRIFSNIAHRLDRYPSCLNH
                     RDYHSWNLMIHDDAVAVIDFQDALLAPPQYDVASLLNDRVTDSVIRPHMEDQLVSYYL
                     RQRGDIEKRAINNDEFREIYLLSAIQRDLKVVGRFYYLDLVKGKPVYRKFIAPTIRRL
                     KRNLARLPQLEPIIPILAEHYEEMR"
     gene            730..1476
                     /locus_tag="VF089_12985"
     CDS             730..1476
                     /locus_tag="VF089_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX6174925.1"
                     /translation="MKAMVLAAGQGTRLRPLTDTQPKALVPVGGRPMIEYCLLLLRHY
                     GIRDIVINLHHRGEQIENRLADGSGLGLQISYSVEPELLDTGGGLLKAKPFLEDDTFI
                     VINTDVLIDLHLDQLLEFHQKQQAAATLVLRPDKDADQYGSMDIDERGRICRFLDTRM
                     PAQSPGPTRKLMFTGVQVLEPKVFDYMDMGLAAHKFSTTRQTYPRMVAEGQPLYGFCF
                     DGFWQDLGTAERIRQVEHTLGAGEIKLHYL"
     gene            complement(1491..2024)
                     /locus_tag="VF089_12990"
     CDS             complement(1491..2024)
                     /locus_tag="VF089_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX6174926.1"
                     /translation="MFRKLCAIMLMNLAAACSMSTQPASQATAELKDKDGKAVGMATF
                     RESGRGVTVNLDVKGLTPGLHAVHVHAVGKCEPPAFTSAGGHFNPAQKKHGHKSPEGA
                     HAGDLPNMLVAKDGTGRFEVFTDGMTLKLGATSVFDKDGSALVIHVGIDDYTTDPTGN
                     AGDRAACGVITLSQSRM"
     gene            complement(2063..2803)
                     /locus_tag="VF089_12995"
     CDS             complement(2063..2803)
                     /locus_tag="VF089_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX6174927.1"
                     /translation="MLSVALVLGLLTALANILGSALAVLQREPSRRFTAAALGLSGGF
                     ILAAALLEMLPESLSRGPSMPGYVAVGYLSLFLIEQFTNVHLHSLPDDEHVVPVPVAA
                     GFASLVAFNVHDFIDGLAIGAGMIAKTDLGILIFLSVLVHEIPAGFVIAAIMRAAGWS
                     RWSAMVAGGSLGVVTIIGIVLPFWAWELEPFFADVLLAFAAGNFIYVGATLLVPLSES
                     GKSRWITLLVVFGFAIFYISRKLVESFF"
     gene            3300..3479
                     /locus_tag="VF089_13000"
     CDS             3300..3479
                     /locus_tag="VF089_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174928.1"
                     /translation="MIAKLNPLEVSTDGAKEVILKYHWLDRLVSSPPATINPVKSDDD
                     PIPFIKVIDAPRSLF"
     gene            complement(3546..5612)
                     /locus_tag="VF089_13005"
     CDS             complement(3546..5612)
                     /locus_tag="VF089_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6174929.1"
                     /translation="MKKASRQQERKVKPAKASGGAAASPDRIHPEGTRLWFLTIFTVA
                     FTLRLVYLFEIDTIPLFEHLAGDARTYYEWGRRIAAGDWLGAGVFYQAPLYPYFLGVL
                     QFFFGETLWLVRLIQITLGALSCSLIFLIGQHLFSRPAGIISGFILAGYGPALFFDGL
                     IEKSILDLFLLTVILFLIFSPGAEANPAKWFAVGAVLGLLALSRENALILVPVVPCWI
                     ALSSAPVLREARLRRIGLFFAGLILVVLPVGLRNLGAGGEFKLTTSQLGPNFFIGNNP
                     AADGTYNSVTDIIGEPQLEGPDAKRLAERALQRSLSPGEVSSYWLGQSFEYIKTQPLD
                     WLRLLVKKWMLLWNGREIEDSDDFYIYSQWSTLLGALGRLNHFGVLAPLAAVGLFVSL
                     GEWRRLWLLYLMILALAGSVAVFYVFGRYRFPLIPLLVLFAGAGSMQIISFYKHRRWN
                     PLFGAMAVFCACAIFTNWPLSEYSGPGAAGYNNLSNAYYKQGRVEEAIQTALKAIEVE
                     PNYGVAHYNIGNIYAGQGKFALAQHHFEEALRIYPNYAEVHSNLGQLLAERGNLPESI
                     EHFRKAIALDSALFRAHLNLGVALAKQGQIDEADAPLREAARLNPQSAETRFYLGSVY
                     AAQNRFDFAAQSLKEALQIEANFAAAHESLARVLSAQGKREEAMAHYQEAIRLIGGRR
                     TGPKAP"
     gene            5932..7374
                     /locus_tag="VF089_13010"
     CDS             5932..7374
                     /locus_tag="VF089_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX6174930.1"
                     /translation="MAYNSLQEFVQVLERHGELRRITYPVKAELEITEIADRVMKKNG
                     PALLFENVPGKQIPVLINAFGSQKRMALALGVDDIEEVAGEVQKLVQTKPPKSFSDKL
                     HLLSELLKLAGIPPKIVSNGECQQIIHRQPDLSILPVLTCWPGDAGPFITLPMVFSKD
                     PAKGTRNVGLYRMQVYDSRTTGMHWHLHKVGARHYQHQKEQSRRMELAVCLGGDPAMI
                     YAATAPLPDQIDEVLFTGFLRKKGVRLVKGVTVDVEVPANSDIVIEGYVDPTEPLRRE
                     GPFGDHTGFYSLADDYPVFHVTCMTHKEHPIYPTTIVGRPPMEDAYMGKATERIFLPL
                     LRLTFPEIVDMNLPVHGVFHNLAIISIKKEYPAHARKIMHALWGMGQMMFTKILVVVD
                     HDINVHDLSEVTWVAGNHIDPQRDTVIVEGPVDVLDHAAPVLGYGSKIGIDATRKWRS
                     EGFEREWPAVIVMDEKTKKHIDSIWQKLGI"
     gene            7463..8353
                     /locus_tag="VF089_13015"
     CDS             7463..8353
                     /locus_tag="VF089_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX6174931.1"
                     /translation="MLVEKIRSYAGLVRFSHTVFALPFAFASLVLAWPSHPITLRTVF
                     WILVAMVGARTAAMGFNRLADRKFDALNPRTQGWELPKGTVKTWEAGLLTIVASTIFV
                     FGAYQLNWTCFMLSPVALAVIFFYSLTKRFTWTSHLFLGAALSLAPVGAWLAVAGWPI
                     DLQELVIPLCLGLAVIFWLAGFDIIYSLQDREFDRAHGLHSIPVRFGVAWALRLSTFF
                     HVCTIVFLLLVGIAADLGTIYWVGCLAVSLILMWEHRIVRPNDLSRINRAFFDFNAYV
                     SIGYFLITLADVLVSSTSVG"
     gene            8350..>8787
                     /locus_tag="VF089_13020"
     CDS             8350..>8787
                     /locus_tag="VF089_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6174932.1"
                     /translation="MISLTLISTLAALGILGGFFAGLLGFGGGVMMFPLLYYIPPLLG
                     LSRLEANTVAAVVISQVFFSTIIGGMAHFRQRRVHRRIVLGAGPASAMGSFAGAVSSA
                     NISEDLLLVLFGLITVLVLGMMFLPAPDQIHEDLSQEQVVMALL"
BASE COUNT         2079 a         2386 c         2325 g         1997 t
ORIGIN      
        1 cgatacagcc ttatgggacc gggtccaagg actgccagaa gaagagatcc ttacatggta
       61 tgaaaaagcc gtcgatgagc ttttgatgct gcaaatccga ggcacagcgg cccgggacga
      121 agcctgcgtt gcatttcagc agcgatttga tttccggctt tacatgtggg agttcgatca
      181 tttcctcgaa tacggcttgg agaagcgacc gggcactcat gtgcatgcaa cggcgatcga
      241 gacgctgcga agaatatttt cgaacattgc ccatcgcctg gatcgctatc ccagctgttt
      301 gaatcaccgc gactaccaca gctggaactt gatgattcac gacgatgcgg tggcagtgat
      361 agatttccaa gacgccctgc tggcgccgcc tcagtacgat gtggcctcgc tgctgaacga
      421 ccgggtcacg gacagcgtca ttcgcccgca catggaagat cagcttgtca gctattactt
      481 acgccagaga ggcgatattg agaagcgtgc gatcaacaat gatgagttcc gagagatcta
      541 tctgctttcg gcgattcagc gcgatctcaa agtagtcggc cgattttact atctcgatct
      601 tgtgaagggc aagcccgtat atcgcaaatt catcgcgccc acgatccgtc gcttgaagcg
      661 gaatttggcg cgcttgccgc agctcgaacc gatcattccg atcttggctg agcattatga
      721 ggagatgcga tgaaggcgat ggttctagcc gcagggcagg gaacacgtct gcggccgctg
      781 accgataccc agccgaaagc tctcgtgccg gtcggcggcc ggcccatgat tgagtattgc
      841 ctgctgctct taaggcatta tggcattcgc gacatcgtca tcaacctgca tcatcgtggc
      901 gagcagatcg agaaccggct ggccgatggc agcgggctcg gattacagat cagttattcc
      961 gtcgagccgg agctgttgga taccggcggc ggtctgctca aggccaagcc attcttagaa
     1021 gacgacactt tcatcgtcat caataccgat gtattgatcg atttgcatct cgaccaattg
     1081 ctagaatttc accagaagca gcaagccgcg gcaacgctgg ttctgcggcc ggacaaggac
     1141 gcggaccaat acggctcgat ggacatcgac gagcgaggtc ggatctgccg atttcttgat
     1201 acaagaatgc cggcgcagtc gcccggcccc acccgcaagt taatgttcac cggcgtacag
     1261 gtcctcgagc cgaaggtctt cgattacatg gacatgggac tggcagcgca taaattcagc
     1321 accacccggc aaacctaccc gcgaatggtg gctgaagggc aacctctata tggtttctgt
     1381 tttgacggat tttggcagga ccttggaact gccgaacgga tccggcaagt agagcacacc
     1441 ctcggcgcgg gcgagatcaa gttacattac ctctagtcgt tcggagcttg ctacattcgg
     1501 gattgactca gcgtaattac gccgcatgcg gctctgtcgc ccgcattgcc ggtaggatct
     1561 gtggtgtagt cgtcaattcc gacatgaatc actaacgcgc tgccgtcttt gtcgaaaacc
     1621 gatgtcgcgc cgagtttaag cgtcatcccg tcggtgaaaa cttcaaaacg gcctgtccca
     1681 tccttggcaa caagcatatt gggcagatcc cctgcgtgag cgccttcggg acttttgtga
     1741 ccgtgtttct tttgcgccgg attgaaatgg ccgccggcgc tggtaaaggc cggcggctcg
     1801 catttaccga ctgcgtgaac atgaaccgcg tgcaagcccg gagtgagtcc cttgacatca
     1861 aggttgaccg tgactcccct cccgctctca cgaaacgtag ccattccgac cgccttgccg
     1921 tccttatcct tgagttcggc agtggcttga gaagcgggct gggtggacat cgaacaagcc
     1981 gccgccaaat tcattaacat gattgcgcac aattttcgaa acatagcact cccccttttg
     2041 attcgtcaaa gaatctacaa gcttaaaaaa aggactcaac gagcttcctc gaaatgtaga
     2101 aaatggcaaa cccgaacacc accaggagtg tgatccaacg cgatttaccc gattcggaga
     2161 gcgggacgag cagagtcgca cccacataga taaagtttcc ggcagcgaat gccaagagga
     2221 catcggcaaa aaatggttcc agttcccagg cccaaaatgg caggacaata ccaatgatcg
     2281 tgacaacccc taagctgccg ccggcaacca tagcagacca ccggctccag cccgctgcac
     2341 gcatgatcgc ggcgatcaca aagccggcgg gaatttcgtg aactaacacc gagagaaata
     2401 tcagtattcc caaatcggtt ttggcgatca tgccggcgcc gatagcaagt ccgtcaataa
     2461 agtcgtgaac gttgaacgca acaagggatg cgaatccggc ggcgactgga acaggcacaa
     2521 cgtgttcgtc atcaggcaga ctatgcaggt gaacgttggt gaactgttcg atcaagaaca
     2581 gcgagaggta tccgaccgcc acatagcccg gcatcgatgg tccacgggat aagctttccg
     2641 gcagcatctc aagcagcgcg gctgcgagaa taaatccgcc actcaaacca agtgccgccg
     2701 cggtaaagcg ccgcgacggc tcgcgctgaa gcaccgcaag agcgctgccc aagatattcg
     2761 ccaaggcggt caacaatccg agaaccagag cgacactaag catccgtctc ttctgacaaa
     2821 cttgatcttg ggatgcaaat gattgcttga gaatggctcg gcagagggcc acacttcgca
     2881 aaccgtctac tcggaactga gcgcgaaagc gcacggccag catcgcaagg aggaactcgg
     2941 ccgcaagcaa cggtactgct gtgacggacg tatggcgaaa taactggcgt gcgttaggtt
     3001 aacggaagtg ttccgatgtt tacacactcc cagtattcta aagattgtac atgggctttt
     3061 gttttctcca gccaaatacc gctagattat tggggcgaga tatacaggct ttcacggatg
     3121 aagaaatcga aaaatacttt cggctctaca atatcgccgc ggtggttgcc tttcttccgg
     3181 cctcaatgag acggctaacg tcggtccctg gattaatcac gctcgatcgg cgcatcggtc
     3241 cggtgcatct gatgagggtg aactagcggc cagattggtt tgtacaagga caagggcgag
     3301 tgatagccaa actcaacccc ctggaagtct cgactgacgg agctaaggaa gtgattctaa
     3361 aataccattg gctcgaccgc ctggtcagca gcccaccggc aaccatcaac ccggttaaga
     3421 gtgatgacga tccgattccc ttcatcaagg tgatcgatgc gccacgatcc ttattctgaa
     3481 agtcctacgc tgaacgtgtg tcaaagtcgg atgtttcccc gagcaacgcc gttaaaacgc
     3541 attggctacg gagccttggg tccggttctt ctgccaccga tcagcctgat ggcttcctga
     3601 tagtgcgcca tcgcctcttc cctcttacct tgagctgata gcactcgtgc gagactctcg
     3661 tgtgctgcgg caaaattagc ctcaatctgt aacgcctctt tgagtgactg cgccgcaaaa
     3721 tcgaagcgat tctgagccgc gtatacgctg ccaagataga accgagtctc cgccgattgc
     3781 ggattcagcc gggcggcttc tcgcagtgga gcatccgctt cgtcaatctg gccttgcttc
     3841 gccagggcga ccccaagatt cagatgggcc ctaaagagcg ccgagtcaag cgcgatggcc
     3901 ttgcgaaagt gctcgatact ctcgggaaga ttacctcgtt ctgcgagaag ctgtccaaga
     3961 ttgctgtgga cctcagcgta atttggatag atccgcaacg cctcttcaaa gtgatgttgt
     4021 gctagcgcaa acttaccctg tccagcatag atattgccga tattgtaatg ggcgactccg
     4081 tagttcggtt ctacctcgat ggccttaaga gctgtttgaa tagcctcctc gaccctgccc
     4141 tgtttgtaat aggcattaga taggttgttg taacccgccg cacccggccc gctatattcg
     4201 gacaggggcc agttagtgaa aattgcgcag gcgcaaaata cagccatcgc tccgaacagc
     4261 gggttccagc gtcgatgttt atagaaagag attatttgca tcgatccggc gccggcaaaa
     4321 agaaccagca aggggatcag tggaaagcga taacggccaa atacgtaaaa tacggccaca
     4381 cttcctgcaa gcgccaaaat catgaggtag agcagccata gtcgacgcca ctcaccgagc
     4441 gaaacaaaga gacccacagc ggccagagga gccagaacgc caaaatgatt cagccgaccg
     4501 agcgcgccaa gcaacgttga ccattggcta taaatgtaga aatcatccga gtcttcaatc
     4561 tcccggccat tccataacag catccacttc ttgaccagca gacgcagcca atccaacggt
     4621 tgtgtcttga tgtactcgaa cgattggcct aaccagtagc tcgatacctc gcctggagat
     4681 aagctacgtt gcagcgctct ttccgctaat cgtttggcgt cgggcccttc gagttgcggc
     4741 tcgccgataa tgtccgttac cgagttgtag gttccgtccg cggccgggtt gttgccgatg
     4801 aagaagttcg gccccagttg tgacgtcgtt agtttaaatt ctcctcccgc accgagattg
     4861 cgcagcccga ccggcaggac gactaaaatc aaaccggcga agaacaagcc gatccggcgc
     4921 aggcgtgctt ctcgcaacac cggcgcggaa gagagcgcga tccagcacgg caccacgggt
     4981 accaagataa gcgcattttc gcgcgacaag gctaatagac caagcacggc gccgacagca
     5041 aaccacttag ctgggttcgc ttcggcaccg ggggagaaaa ttagaaacaa gataaccgta
     5101 agcaaaaata aatcgagaat acttttttca atcaggccgt cgaagaacag cgccgggccg
     5161 taacctgcca gaataaaccc cgaaatgatt cctgccggac gtgagaacaa gtgttggccg
     5221 atcaagaaga tcaacgagca cgaaagcgcc ccaagcgtaa tctggatgag ccgcactagc
     5281 caaagagttt cgccgaaaaa gaattgtagt actccgagaa aatagggata taagggagcc
     5341 tgatagaaaa ctcccgcgcc cagccagtcc cccgccgcga tccgtcggcc ccactcgtag
     5401 taagtcctgg cgtctcccgc gagatgctca aacagcggaa tcgtatcgat ctcgaacagg
     5461 taaacgagcc ggagagtaaa cgcgacggta aaaatagtca ggaaccaaag ccgcgtcccc
     5521 tccggatgga tccggtcagg cgatgccgct gcgccgccgg atgcttttgc cggtttgact
     5581 ttcctttcct gctgtcgcga cgcctttttc atttagatct tcttacgact gaagaaatct
     5641 cgaccatact cactgcctgc caattattgg gggggggaga agttgtgact attcaacgat
     5701 aatgctgaaa tgtgttggtt caatgcaagg aggcggatta tcgattccga tcaaagtatc
     5761 cgacgatccc aagtcaacaa taatagttct taagaccagg ttaaatccaa ctaagtccaa
     5821 cagcgagcca catccatata gcggactgcg aagtccgccg cgcgcagggt tacttgactt
     5881 gcgccttcct gtcggcttat tgcttttcat tcgaaagata tcagggagca catggcatat
     5941 aacagtctgc aggaattcgt acaagtttta gaacgccacg gcgaactcag gcgcattacc
     6001 tatccggtca aagccgagtt ggagatcaca gagattgccg atcgcgtgat gaaaaaaaac
     6061 ggtccggcgt tgttgttcga gaacgttccc ggcaaacaga tcccggttct aatcaatgcc
     6121 ttcggttcgc aaaaacgcat ggctctggcc ttgggcgtcg atgatataga ggaagttgcc
     6181 ggcgaagttc aaaagctggt gcaaaccaaa ccgccgaaat cattcagtga caagctgcat
     6241 ctcctctcag aattgctaaa actcgcgggc atccccccca aaattgtcag taacggcgaa
     6301 tgccagcaga tcattcaccg gcaaccggac ttaagcatat tgcccgtgct cacctgctgg
     6361 ccgggagatg ccggaccatt tataactttg ccgatggttt tttcaaaaga cccggcgaaa
     6421 ggaactcgaa acgtaggtct ttaccggatg caagtttacg attcgcgcac aaccggcatg
     6481 cactggcact tgcacaaagt aggggcgcga cactatcagc atcagaaaga gcaaagccgg
     6541 cgcatggagt tggctgtctg tctgggcggg gaccctgcaa tgatctacgc cgcgacagct
     6601 ccgctgccgg atcaaatcga tgaggtactc tttaccggtt ttttgcgcaa gaaaggtgtg
     6661 cgcctggtta agggtgtcac tgtggatgtg gaagttccgg caaactcgga tatcgtgatt
     6721 gagggctacg tcgatcctac cgagccactg cgccgcgaag ggccctttgg agatcatacc
     6781 ggtttttatt cgctggccga cgactatccc gtattccacg tcacctgcat gacccacaaa
     6841 gagcatccga tctacccaac gaccattgtc ggtcgacctc cgatggaaga tgcttacatg
     6901 ggtaaggcga ccgaaagaat ctttctgccg cttttgcgtc tgacgtttcc cgagattgtc
     6961 gatatgaatt tgccggtgca tggagtgttc cataacctgg cgatcatctc gatcaagaaa
     7021 gaataccccg cccatgcgcg caagatcatg cacgctctct ggggcatggg tcagatgatg
     7081 ttcactaaaa ttttggtagt cgttgatcac gacatcaacg tgcacgatct ttccgaagta
     7141 acctgggtag cgggtaacca tatcgatccg caacgcgaca cagtcatcgt cgaaggtcca
     7201 gtggacgtct tggaccatgc ggcgccagtt ctgggctatg gatcgaagat cggcatcgat
     7261 gcaacgcgca aatggcgcag tgaaggattc gagcgcgaat ggcccgcggt cattgtcatg
     7321 gacgaaaaga ctaaaaaaca tatcgactca atttggcaaa agctgggcat ttgaaatgct
     7381 catcagaaac tcctccgctc gtccaggcca tgaatgcgac tcgcacttgg cttaccagcg
     7441 ctagcttcca ggcttgaacc cgatgctcgt cgaaaaaatt cgcagctacg caggtctcgt
     7501 ccgtttttct catacagttt ttgcgctgcc gttcgcattt gcttcattgg ttcttgcctg
     7561 gccaagccat ccgataactc tcagaacagt cttttggata ttagttgcca tggtcggcgc
     7621 ccgtaccgcc gccatgggtt ttaatcgttt ggccgacagg aaattcgacg cattgaatcc
     7681 gcgcacccag ggttgggaac tacctaaggg aacggtaaag acgtgggaag cgggacttct
     7741 gacgatcgtc gcatcgacca tctttgtttt cggcgcttac caactcaatt ggacttgttt
     7801 catgctgtcg cctgtggctt tggcggtcat tttcttctat tcgctcacta aacgtttcac
     7861 ctggacatcg caccttttcc tcggagccgc tctttcgctt gcgcccgtcg gcgcttggct
     7921 ggcggtcgct ggttggccaa ttgatttgca ggagttggtg attccgcttt gtctcggatt
     7981 agcagtgatc ttttggctgg cggggttcga tatcatttat tcactgcagg atcgcgaatt
     8041 cgatcgcgcg catggattac actcgattcc ggttaggttc ggcgtcgcct gggcattgcg
     8101 cctctcaacc ttctttcatg tctgcacgat agttttcctc ctcctcgtag gtattgccgc
     8161 tgatctgggg accatatact gggtcggctg cctggcagtt tctctcatct tgatgtggga
     8221 gcaccgtatt gttcggccta atgatctttc gcgaatcaat cgcgctttct tcgatttcaa
     8281 tgcttacgtc agcatcggtt atttcttaat tacactggcc gacgttttgg tctccagcac
     8341 cagcgtcgga tgatcagcct gacactcatc tccaccctag cggcactggg aatcctcggc
     8401 ggattctttg cgggcttgtt gggcttcggc ggcggcgtca tgatgttccc tcttctgtat
     8461 tacattccgc cgctgttggg tctgtcccga ctggaggcga atacggtggc cgctgtggtg
     8521 atcagccagg tatttttctc cactatcatc ggcggtatgg ctcatttccg gcagcggcgg
     8581 gtgcaccggc gaattgtttt aggagcgggt cccgcttcgg cgatgggttc atttgccgga
     8641 gcagtttctt cggcaaatat ttccgaagat ctcctgcttg ttttgtttgg actcatcacg
     8701 gttctcgtgt tgggcatgat gttcctgcct gcacccgatc agatccacga ggatctttcg
     8761 caagagcagg tagttatggc gttgcta
//
LOCUS       DASUTG010000564         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1422913, whole genome shotgun sequence.
ACCESSION   DASUTG010000564 DASUTG010000000
VERSION     DASUTG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422913"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VF089_13025"
     CDS             complement(<1..573)
                     /locus_tag="VF089_13025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX6174933.1"
                     /translation="MRLILASTSPRRREILALLGLPFQVIKPAFEEVTSWERSIEEEV
                     IEFAVGKAESVARQNPQAIVIGSDTMISLDGAKIGKPHDLSDARAMLCKLAGRTHLIF
                     TSVAIIDGLGVAGLKVIEKVAVKMRDFSEAEVEDYLTCGESLDKAGAYSIQGEGRQLI
                     ASIDGDYLAAVGLPLKPIAEYLKDRGIALPL"
     gene            complement(601..1731)
                     /locus_tag="VF089_13030"
     CDS             complement(601..1731)
                     /locus_tag="VF089_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX6174934.1"
                     /translation="MDGVTDASFRHITAKYGRPDVTFTEFVNIQAASHAAHTLIKDLT
                     YSEIERPVVAQIYGRSPEMFYRVSHIVCELGFDGLDINMGCPAKKVAAAGCGAALIRE
                     PQLARSIIRAAKLGIKDWCAGQTLRDIQVEPELIERIIMANRLRTGTDGPVERLPIPV
                     SVKTRLGYDRIVVEDWVSTLLDENPAAISLHGRTLQQGYKGNADWGAIARAVKVAGES
                     ETLILGNGDLQDMTQVRRRVSETQADGVLLGRAAQGNPWIFRAKHDVKRALAENRESS
                     IATTPVSLEERFRVILDHSIHHQQASGETFVGIRKHLTWYCRGFRGAAEMRSQMVRVK
                     TAADVKQCLVNFTVQSLEAERRLNDWHEDPPPILTPGRYATV"
     gene            2174..4633
                     /locus_tag="VF089_13035"
     CDS             2174..4633
                     /locus_tag="VF089_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX6174935.1"
                     /translation="MRKWVFIAGGILLIILIAAVVAVLNVNSYIQRNREYLIQQAEKA
                     VGRQIEVQNIEVNLWNGIGIRFEDFRLADDPAFSSDPFVQAKSLQANVKLLPLLRKDV
                     QVKRLILHEPGINIIRNRRSVYNFASLGEPDKKKDKETEDAKDKAARERAAVLISLVE
                     ISGGVARYRDLGDGTDLAVQQLDVRAQDLNFDRPVSINLAAAVFASKQNLNLKLMIGP
                     LSAESDYRNVAFDSHLDIDPLDVSQLKKAVPKLLTALPKELEVGGIFRMTGLKIKGTL
                     RDFAFNGGIEGTQGSIRYADSFHKPAGIPLVLESAAQYSNDKIAIQNAALKLHTLPLQ
                     VKGDIALRDRPVLNLSFDSEPTSLEGWGKLIPAVVNYDLNGKMGVHAAVRGPVGAGRL
                     PQVDGTLSLQSAGIKIPELPKAIENLNAQVKFSEGRAEARETSFNLGKSLIRFTAVIE
                     RFSPLTLAYKLNTAELALADIQPSLPPERSSDVLRDLGSDGQVTTTNGQTLVAGRILS
                     GDGTIYNMAFKNLDANLSYAKNNLVLRSFRANLLNGAVQLNGEYGFDESPRFSMASEI
                     KGIDLVQMYQYFTQDQPDMRGSLNGNLKLSGQGDQWAAIKPTLRGDGQTEVLKGTLLN
                     FNIADAVLSGATGIPGLTNLINPAVRKKYPETFEAKDTVFKDLQTGLDIGNSRVNLKN
                     LRIAAAQFRVAGDGWVDFERRVDFRGVIRFSPALSSDIAGAVREIRLLFNKNNELEIP
                     FTLSGQMPNVRPRPDASYLTKSLQRGLFQRGAEELQEQLFGPKERRAPDRQADQEQPS
                     PKSPEDLIRRGLEGLFGRQKR"
     gene            complement(4651..>5253)
                     /locus_tag="VF089_13040"
     CDS             complement(4651..>5253)
                     /locus_tag="VF089_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6174936.1"
                     /translation="VLRGARGLLSEIRPDLRSTYLALVLATVASQSQRILVQSILPIY
                     AGFYLQLSPTQIGMLFSISGIIVFAMIIPAGLVMDRIGRKWCTVPSTGIPALVFVLIP
                     LTSSFTQLSILVGLSGLAQGLSLGSIATSTYDVVPSHVRGRLQAARRTVAELGSGLAP
                     MIGGYLANAYNPGVPFLVYAPLLLFSAVLLGLVGKETLDR"
BASE COUNT         1286 a         1393 c         1378 g         1196 t
ORIGIN      
        1 tagcgggaga gcgatgccgc ggtcctttag atactcggct atcggtttca acgggaggcc
       61 caccgccgcg agatagtcgc cgtcgatgga ggctatcaac tgacgcccct caccctgaat
      121 cgaataggcg ccggccttat cgagggattc gccgcacgta agatagtctt caacttcagc
      181 ctcggagaag tcgcgcatct ttaccgcgac tttctcgatg accttcaggc cagcgacacc
      241 caaaccatcg atgatcgcta cactggtgaa gataagatgg gttcgtcctg caagcttgca
      301 cagcattgct cgagcgtcgg acaggtcatg cggtttgccg attttcgccc catcaagtga
      361 gatcatcgtg tcactgccta tcacgatagc ttgcgggttc tgcctggcca ccgattcagc
      421 tttgccgacg gcaaattcta taacctcttc ttcgatgctg cgctcccacg aggtaacttc
      481 ttcaaaagcc ggcttgatta cctgaaatgg caggccgaga agcgcgagga tttcccggcg
      541 gcggggtgag gttgaagcga gaatcagcct catggtgtgg aatgggttcg acggcttggt
      601 ttagacggta gcgtagcgcc ccggtgttaa aattggtggc ggatcttcat gccagtcgtt
      661 taatctgcgc tctgcctcga gagattgaac ggtaaaattg actaagcatt gtttgacgtc
      721 ggcggcggtt tttactcgaa ccatttgcga gcgcatttcc gccgcgccgc gaaaaccgcg
      781 acaataccag gttagatgct ttcgaattcc gacaaaggtt tcacccgatg cctgctggtg
      841 atggatgcta tggtctagaa tgacgcgaaa gcgctcttcc agactaacgg gcgtggtggc
      901 aatggatgac tctcggtttt ctgcgagcgc gcgttttacg tcatgcttgg ctctaaagat
      961 ccatgggttg ccttgcgcgg cgcgcccgag aagaacgccg tccgcctggg tttcagagac
     1021 acgccgccgc acctgcgtca tatcctgcag atcgccgtta cctagaataa gcgtttccga
     1081 ttctccggcg actttcacgg cgcgcgcaat cgcgccccaa tccgcgttgc ctttgtatcc
     1141 ctgctgcaaa gtgcggccgt gcagagagat agcagccggg ttttcatcca acaaggtgga
     1201 aacccaatcc tcgactacga tacggtcata gcccaagcgg gttttgaccg aaactgggat
     1261 tggcagacgc tcaaccggac catccgttcc tgtgcgtagc cggttggcca ttatgatcct
     1321 ttcgatgagc tcaggttcta cttgaatatc acgcagcgtc tgtccggcgc accagtcttt
     1381 gatacctagc tttgccgctc tgatgatcga ccgagccaat tgcggctccc gaatcagcgc
     1441 cgcaccgcaa cccgctgccg caaccttctt cgccgggcac cccatgttga tgtctaaacc
     1501 gtcgaaacca agctcacaga cgatgtgtga gactctatag aacatttccg gcgaacgccc
     1561 atagatctgc gcaactaccg gccgttcgat ttcactatag gtcagatctt taatcagcgt
     1621 gtgcgcggcg tgagaagcgg cttggatgtt aacgaactcg gtaaatgtca catcgggcct
     1681 tccgtatttg gctgtgatgt ggcgaaaaga cgcgtcagtg actccgtcca taggcgagag
     1741 gccaatgatg ggccagctaa tccgttgcca aaaattcgtg tcgttacgag tggattctaa
     1801 agatcgtgaa cgtgaaatca ctattgttcc cttatccgag gcggatcaca aagcttaatc
     1861 catgtgagcg cctaaaccaa gctaccccca tagaggcgat gacaacattc cacgatttcc
     1921 tctgatcggc aatttacagc gacagtttgc acacatccat gtggcaatta ataattgcga
     1981 ccggctgcag atcgccgggg agcttagact gaaactggat ccagtcgaca agatgccacg
     2041 gtttcatctg cgcgtttttc agttacacta tattacttgt gcatacggat cgcatttgcc
     2101 gtcggcagcc acagctcgat tgcagttact cccgccgctt gtatcgatcc tgcgcagaaa
     2161 ggagatggga actatgcgta agtgggtatt catcgctggc ggtatcctgc tgataattct
     2221 gatcgccgct gtcgttgcgg ttttgaacgt caattcatac atccaacgca atcgcgagta
     2281 tctcattcaa caggcggaga aagctgttgg acgccaaatc gaggtgcaaa atatcgaagt
     2341 aaatctttgg aacggtatcg gcatccgttt tgaagatttc agattggccg atgatccggc
     2401 cttttcctcc gatcctttcg tacaggcgaa aagtcttcag gccaatgtca agcttctacc
     2461 tctcttgcgc aaagatgttc aggtcaaaag actcattctc cacgagcccg ggataaatat
     2521 cattcgaaat cgacggagtg tttacaactt cgcgagtctt ggcgaacccg acaagaaaaa
     2581 ggacaaggaa acagaagatg ctaaggacaa agcggcgcgc gaacgtgccg ctgttttgat
     2641 ttctctagtt gagatatccg gcggagttgc acgttaccgt gacctgggag acgggacgga
     2701 tttagcggtt cagcaactcg atgtacgggc gcaagatctt aattttgatc ggcctgtttc
     2761 aatcaatctc gctgcggcag tttttgcttc aaagcagaat ctaaatctca agctaatgat
     2821 tggaccgctc tccgctgaga gcgactaccg caacgttgca ttcgatagcc atctcgacat
     2881 cgatcctctc gatgtgagcc agctaaaaaa agccgtgccg aagctcctca ccgcgttgcc
     2941 gaaagagctg gaagtcggcg gcatttttag gatgacgggg ctgaaaatca aaggaacgct
     3001 gcgtgatttc gcgtttaacg gcggcattga aggcacccaa gggtccattc gatatgctga
     3061 ctcttttcat aaacctgcgg gcattcctct agtgctggag agcgccgccc agtacagcaa
     3121 tgataagatt gccatccaaa acgctgcgct aaagcttcac acgctgcctt tacaggtcaa
     3181 aggtgacatt gctctgcgcg atcggccggt gctcaaccta tcgtttgatt ccgaaccaac
     3241 ctccctagag ggttggggga aactgattcc ggcggtagtt aattacgacc tcaacggcaa
     3301 aatgggtgta catgcagcgg ttcgtgggcc cgtcggagca ggaagacttc cgcaggtgga
     3361 tggaacccta tccctgcaga gcgccggcat taagattcca gagttaccta aagctatcga
     3421 gaatctaaat gctcaggtga agttctctga aggacgcgcc gaagctagag aaacgagctt
     3481 caatctcggt aaatcgctca tccgtttcac tgcagtcatc gagagattct cgccacttac
     3541 tctagcgtac aagctgaaca ctgccgagtt agcgctggcg gatatccaac cttctctgcc
     3601 gccggagcgt agcagcgatg tcctgcgcga tctcggtagt gatggacagg taaccaccac
     3661 caacgggcaa accctggtgg cgggcaggat tctttctggt gatggaacaa tctataacat
     3721 ggcttttaaa aatcttgacg ctaatctctc ctacgcgaaa aacaacctgg ttttgcgtag
     3781 ttttcgcgct aacctgctaa acggagccgt acagctaaac ggcgaatacg ggtttgatga
     3841 gagtccacgg ttctccatgg cgtcggaaat taagggcatc gatctagtgc aaatgtatca
     3901 atacttcact caagaccagc cggacatgcg cgggagcttg aacggcaatc tcaaactatc
     3961 gggacagggt gaccagtggg cggcaatcaa acctactctg cgtggggacg ggcaaaccga
     4021 agtcctaaag ggtaccttgc taaattttaa tatcgccgat gccgtgctct ccggagccac
     4081 cggaataccg ggtctgacaa atctcatcaa tccggctgtc cggaaaaaat accctgaaac
     4141 gtttgaggcc aaggacacag ttttcaagga cttgcagaca gggctcgata ttggcaacag
     4201 ccgggtaaat ttaaaaaatc tgcgaatcgc cgcagcccag ttcagggtcg cgggagacgg
     4261 ttgggtcgac ttcgaacggc gggtcgattt ccgcggcgta atccgatttt ctcctgcatt
     4321 gtctagtgat atagccggcg cggtccgaga aataagatta cttttcaaca agaacaacga
     4381 gctggaaatt ccatttacgt tgtcggggca aatgcccaat gtcaggccac ggcccgacgc
     4441 aagttatctg acaaagtcgc ttcagcgcgg tctgtttcaa agaggcgccg aagaacttca
     4501 agaacaactc ttcggcccta aagaacgccg tgctccagat cgccaggcgg accaagaaca
     4561 gcccagtcca aagtcaccgg aagacttgat tcgcagagga ctcgaagggt tgtttggccg
     4621 ccagaagcga taatgaaatt cgcttgtatc tcatcgatcg agcgtctcct tgccgactaa
     4681 tccgagaagt acggcagaaa aaagtagtag aggggcatat accagaaacg gcacaccggg
     4741 attgtaagcg tttgccagat aaccgccaat catcggagca aggccactac ccaactcggc
     4801 cacggttcgc cgtgctgctt gcaatctccc acgcacgtgc gaaggcacca catcgtaggt
     4861 ggaagtcgcg atcgacccca atgacagtcc ttgagccaaa ccggacaatc cgacgagaat
     4921 cgataactgg gtgaagctgc tcgtcagcgg gatcagaacg aacacgagcg ccggaattcc
     4981 ggtgctcggt accgtgcacc actttctccc tattcgatcc ataaccaagc ccgcgggaat
     5041 aatcatggcg aaaacgatta ttccggaaat agagaagagc ataccgattt gagtgggcga
     5101 tagttgaaga taaaatccgg catagatcgg caatatgctc tgcaccagta ttctttgcga
     5161 ctggctggca accgtggcga gcacaagagc cagatacgtg gatcttagat ctgggcgtat
     5221 ctcgctcaac aatcctcttg cgccacggag tac
//
LOCUS       DASUTG010000565         5145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1273013, whole genome shotgun sequence.
ACCESSION   DASUTG010000565 DASUTG010000000
VERSION     DASUTG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273013"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1234
                     /locus_tag="VF089_13045"
     CDS             233..1234
                     /locus_tag="VF089_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174937.1"
                     /translation="MQGPNNRLRVGILSGISFLTVFAVPVHSQTEKLRVAYTVIAPTQ
                     ATLWTANELGLFAKHGLDVELVLLVGAPLAVTALVSGETPIVHAGASAVVTSNLQGSG
                     TVLVAGGANRFPYVLFVTDEIKRVEELRGKKFGVSRIGSADNAAAITVLNRFGIKDTE
                     ITYVQAGSIPSRLAAMQTNALQATLLQAPETLKAKEIGMRPLLDFTKLDVEWQQNGVA
                     TTRDYIKKKPDTVRRFLRAFVEGSAYNLTNPKGAQKILQKYLAIKDDKSVQESYNEIV
                     AKLTLKVPYPTEPGLQLYLDQLKIKNPRAAQAKPSDFTDISFLKELEASGFIEKLYK"
     gene            complement(1369..2004)
                     /locus_tag="VF089_13050"
     CDS             complement(1369..2004)
                     /locus_tag="VF089_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX6174938.1"
                     /translation="MKIISETIVKSNSGKTFEVKKGQIIRVVGESTADYVVFNLRNVK
                     ERFDQARTKVDQGKIFVTTGDVLISKFNNVMQTIVEDTYRGTHDMEKGMCSTSFYKKW
                     GDEIFKIYGDVWKKLGRKKVVAPKHGCWENLARALKPHGVPMEDVPSPLNIFQTMVIN
                     AKTGSMRYSMTRPRPGGDMMDLRSEMDCLVGISACPEGGRGKDLRIVIYKN"
     gene            2149..3300
                     /locus_tag="VF089_13055"
     CDS             2149..3300
                     /locus_tag="VF089_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174939.1"
                     /translation="MINGLRVYDADAHAIMSPAMWHDLPEDFIRRRPRAVRIIDDNDL
                     GRWNTGWLIEGRMEPHVFGPGSHAANTPGIVMEELGTRTNVDKAHGAIPIGCLDLSDP
                     TARLVLLDKLGIDVQFLMPSTLYANITEEPAYEAALYRAYNRYMGRQCTTQSLRLRWA
                     GLLPMRDNRQACEAIEEMQKLGAAAAIVYGTVGNRMLSHSSFAPIWDEFARSGLPLCV
                     HMGSSYAALAELCENILDSNMISKALPANLAFVAIVGHGMLDRYPDLKVGFLEFGAEW
                     IFYMAGRLKHYAVLNKRRMPSSKGLLARDVDDYFKSGRIFVAAEADDAMMTQEMELLG
                     EDQILFSSDFPHDEGRGDAMKEILERHDLSEAQKRKILCDNTVRFFDGK"
     gene            3331..4302
                     /locus_tag="VF089_13060"
     CDS             3331..4302
                     /locus_tag="VF089_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174940.1"
                     /translation="MKPRLAAAWFCTLVLLSVASAHATNVRIAIPGYNITQIAFFVAK
                     ERGYYREENLDVDLIQMTGTLANLALMTGEVPFTSVPAAAMNANLRGANLRVLFSSYE
                     RPLFWLMTRAPIRDIQELKGKKMGVPGFNSAGYFLLREYPSKHGMEPGKDYTLLQAGD
                     SGPRLLALQNNFVDATILPLPWNIMAQDIGMHEAGSMAKSDLIAPSGAIVVREELLRN
                     APAMAEQFTRATLKGLHFALQRRDATIPIITRSLKIKHELAAKGYDSALPALTHDGTF
                     SEESQKKAVEAVLRIAGAKESLSVDRFFNFAITKKVAAELQAKGWKP"
     gene            complement(4387..>5145)
                     /locus_tag="VF089_13065"
     CDS             complement(4387..>5145)
                     /locus_tag="VF089_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174941.1"
                     /translation="IPMYEARHRPEDHRNKSLAAIDQVRRHLDECQPDVIVLASTHWM
                     PRDGFCIDDGAQHKDGCDSSYQGVEPQLFSFPGDPELAALIGGLARAAGLPVRRIHRV
                     AQEHAVWVPAHLLCQERHIQLLPCSIWWRGPREAHRRFGEIIAAAVRQLGRRAFFVAS
                     GGLSHTFDFSKPPEYVVPQGERFDRLAQEWLARGQHGMLLDMSEDDFETWNPEGRAGH
                     LYMMRGALGRDVRGQSLCYQGSNGTGYLTMLFQV"
BASE COUNT         1184 a         1458 c         1389 g         1114 t
ORIGIN      
        1 caagtgctca acaagatcga tttagctcgt gacacccgtc cgtatgagcc gggtaaagac
       61 gacctcgtga tgaccggcag gaattagcag gtcctcaccg actgggccaa gccccgacgg
      121 cgtggcctaa aatgtccgct gcggaatatt ctcaggaagc gaagatttag aagacttcag
      181 cgtggagaac ttattctctg acaaagggtt attcgagggc aggaggagaa acatgcaagg
      241 tccaaacaat cgactgcggg tcggaattct aagtggcata agcttcttga ccgtcttcgc
      301 ggtcccggtt cactcgcaaa cggaaaagct ccgcgttgcc tatacggtca tcgcgccgac
      361 tcaggcgacc ttatggactg ctaacgaact cggcttattc gccaagcatg ggctcgacgt
      421 cgaactggtg ctcctggtcg gcgcgccgct cgcggtcacc gctctggtct ccggggaaac
      481 tccaattgtt cacgctggcg cctccgccgt ggtcaccagt aatctgcaag gatccgggac
      541 cgtcttggtt gcaggcgggg ccaatcgctt tccctacgtg cttttcgtca ccgatgagat
      601 caaacgagtg gaagagctaa ggggcaagaa attcggcgtg agccgcatcg gcagcgcgga
      661 taatgccgct gcgatcacgg tgctcaacag attcggcata aaagatacgg agataacgta
      721 cgtgcaagcc ggctcgatcc cttcccgttt ggcggctatg cagaccaatg cgctgcaagc
      781 gacgctgttg caggcgccgg aaactctgaa agccaaagag atcggcatgc gacctttact
      841 ggatttcacc aagctcgacg tcgaatggca acaaaacggc gtggcgacta ctcgggatta
      901 catcaagaaa aagcccgaca cggtgcgccg ttttcttagg gccttcgtcg agggcagcgc
      961 ttacaacttg accaatccca agggcgcgca aaaaatcttg cagaaatatc tcgcgatcaa
     1021 agatgataag tcggttcagg aatcttataa cgagatcgtc gccaagctaa ccctcaaagt
     1081 tccgtatccc accgagcccg gcctccagct ctacctcgat caattaaaga tcaagaatcc
     1141 cagagcggcg caggccaagc ccagcgattt taccgacatc agttttttga aagaactgga
     1201 agcgagcggg tttatcgaaa agctttataa ataaaaaccg cgttcaatca gttcaagccg
     1261 ttcaaccgct gcgttccgtt taatccccca ctctttcctc ccccgcgacg cggggaggat
     1321 gaaggagggg gcttgaacga tttgaacgtc ttgaacggtt tgaaccggtc agtttttata
     1381 aatcacgatc cggagatcct tcccccgtcc gccttcggga catgcgctga tgccgaccag
     1441 gcaatccatt tcggaacgta gatccatcat gtcgccgccg ggccgcggtc gggtcatgga
     1501 ataacgcatg ctgccggttt tggcgttgat caccatcgtc tgaaaaatat tgagcgggct
     1561 tggcacgtcc tccataggta cgccgtgcgg cttcaaagcc ctggcaagat tctcccagca
     1621 gccgtgcttc ggcgcgacga ctttctttct tcccaatttt ttccaaacgt cgccatatat
     1681 cttgaaaatc tcatcgcccc atttcttgta gaaagacgtg ctgcacatgc ctttctccat
     1741 gtcatgagtg ccgcgataag tatcttcgac gatggtttgc atgacgttgt tgaatttcga
     1801 aatcaacacg tccccggtcg tcacaaaaat tttcccctga tcgaccttgg tccgcgcttg
     1861 atcgaagcgc tctttgacgt tacggagatt gaacaccacg taatcggcgg tggattcccc
     1921 gacgacccga atgatctggc ctttcttcac ttcaaaagtc ttgccgctgt tgctcttgac
     1981 gatggtttcg ctgatgattt tcatcgcttc ctcccagatg attcaaattc tcgccacgtt
     2041 agctgatcga atgccgcaaa agcaagaatc acaggtgcaa acgccagaga ttgacccagc
     2101 cgacggtgag acatataagc gaagagtcaa ttatctaagg agctcccaat gatcaacggt
     2161 ttgagagtct acgatgccga tgcgcatgcg atcatgagtc ccgcaatgtg gcatgacttg
     2221 ccggaagatt ttatccggcg acggcctcga gccgtgcgaa tcatcgacga taacgactta
     2281 gggcgctgga acaccggttg gctcatcgag ggcagaatgg agccgcatgt tttcggtccg
     2341 ggttcccatg ctgccaatac acccggcatc gtcatggaag agttgggtac tcgaaccaac
     2401 gtcgacaagg cgcatggcgc cattcccatc ggctgcctcg acctttccga tccaacggcg
     2461 cggcttgtcc tactcgacaa attgggcatc gacgtgcaat ttttgatgcc aagcacgctc
     2521 tacgccaata ttaccgaaga accggcttac gaggcggcgc tctatcgcgc ctataaccgc
     2581 tacatgggcc ggcagtgcac gacgcaatcc ctgagactca ggtgggctgg gcttctgccg
     2641 atgcgcgata accgccaggc ctgcgaagcc atcgaagaga tgcaaaagct cggcgcggcg
     2701 gcagcaatcg tctacggcac agtcggtaac cgcatgctca gccattcgag ctttgcaccg
     2761 atctgggacg aatttgcgcg gtcgggcttg ccgctgtgcg tgcacatggg ctcgagctat
     2821 gccgctctcg ccgagctgtg cgagaatatt ttggattcga acatgatcag caaagccttg
     2881 ccggcaaatc tcgctttcgt cgccatcgtc ggtcacggca tgctcgatcg atacccagat
     2941 ctcaaggtcg gctttctcga attcggcgcc gaatggatct tctatatggc cgggcgcttg
     3001 aagcactatg ccgtgctcaa caagcgacgc atgccgtcga gcaagggact gctggcgcgc
     3061 gatgtcgatg attacttcaa atcgggacga atcttcgtcg ccgccgaagc cgacgatgcc
     3121 atgatgacgc aggaaatgga gctgctcggc gaagatcaga ttctcttttc gtcggacttt
     3181 ccgcacgacg aaggtcgcgg cgacgccatg aaagagattc ttgaacgaca tgatctttca
     3241 gaggcgcaga agcgcaaaat tctttgcgat aacaccgtgc gcttctttga cgggaaataa
     3301 actcagcggg aaataaaggg ggcatcatcc gtgaaaccgc gtttagccgc tgcttggttc
     3361 tgtaccttag tgcttttatc agtcgcttcc gctcatgcga ccaatgtccg gatcgccata
     3421 cctggttaca atattaccca gattgcgttc ttcgtcgcca aagaaagggg ctactacaga
     3481 gaagaaaatt tggatgtcga tctcattcaa atgaccggca ctctggctaa tttagcgctc
     3541 atgaccggcg aggtgccgtt caccagtgtt cctgcggcgg cgatgaacgc caacttgcgc
     3601 ggcgccaacc tccgcgtgct tttttccagc tacgagcgtc ctttgttttg gctcatgacg
     3661 cgcgctccta ttcgagacat ccaggagctc aagggcaaga aaatgggcgt gccgggcttc
     3721 aactcggcgg gctatttcct gcttcgagag tatccctcga agcatggcat ggagcccgga
     3781 aaggattata ccctgcttca agccggcgat agcgggccgc ggcttctggc gctgcaaaac
     3841 aatttcgttg atgcgacgat tttgccgtta ccgtggaata tcatggccca ggatatcggc
     3901 atgcatgaag ccggctccat ggcgaaaagc gatcttatcg ctcccagcgg cgccatcgtc
     3961 gtgcgcgagg aattgctacg caacgcgccg gcaatggccg agcagtttac ccgcgcaact
     4021 ttgaaaggcc tgcacttcgc attgcaacgg cgcgacgcga cgattccaat cattacgcgg
     4081 agtctgaaga taaagcacga gctcgccgct aaaggttatg actctgctct tcccgcgctt
     4141 acccacgacg gcacattcag cgaagagtcg cagaagaaag cggtggaggc ggtgttacgg
     4201 atcgccggcg cgaaagaatc tctgtcggtc gacagattct tcaattttgc gatcacaaaa
     4261 aaagttgcgg cggagttgca agcaaaaggc tggaagccat aagccttatc gcgcgaaaca
     4321 atcggttatt tgcctatatc gcagtcgcac gttcgcgaac gaatggaaca gttggaacaa
     4381 cgttcatcaa acttgaaata gcatcgtcag ataccccgtc ccattgctcc cctgataaca
     4441 cagcgattga cctctaacgt cccggcctag agcgccacgc atcatataga gatgaccggc
     4501 gcggccttcc gggttccagg tctcaaaatc atcttcgctc atgtctagga gcatgccatg
     4561 ctggcctctc gcgagccact cttgcgcgag ccgatcgaac cgttctccct gcggtaccac
     4621 gtattccggc ggcttggaaa aatcaaaggt gtgagataat ccgccactgg caacgaaaaa
     4681 ggctctgcga cccagttgcc gcaccgccgc cgcaatgatc tcgccgaacc ggcgatgggc
     4741 ttcgcggggc ccgcgccacc agatcgagca cggcaagagc tggatgtgcc gctcctgaca
     4801 gagtaaatgg gccggcaccc agactgcgtg ctcctgcgcg acccgatgaa tacgcctgac
     4861 cggtagtccc gccgcgcgcg cgaggccgcc aatcagcgcg gcgagctctg ggtcgccggg
     4921 gaaactgaag agctgcggtt ccacgccttg ataagaggaa tcgcagccat ctttgtgttg
     4981 cgcgccgtcg tcgatgcaaa aaccatcccg cggcatccag tgagtcgacg ccaagacgat
     5041 tacgtcaggt tggcactcgt ctaaatgccg gcgcacctga tcgatggcag ccaatgattt
     5101 attgcgatga tcttcggggc ggtggcgcgc ctcgtacatg ggaat
//
LOCUS       DASUTG010000566         5597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_236958, whole genome shotgun sequence.
ACCESSION   DASUTG010000566 DASUTG010000000
VERSION     DASUTG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236958"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /gene="purE"
                     /locus_tag="VF089_13070"
     CDS             <1..398
                     /gene="purE"
                     /locus_tag="VF089_13070"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6174942.1"
                     /translation="VPHENLIVSAHRTPAWLAEFAGTAEARGIEVIIAGAGGAAHLPG
                     MAAAQTLVPVLGVPVESAALKGMDSLLSIVQMPGGIPVGTLAIGKAGAINAALLAIAI
                     LGNSRAELREKLRQSRARQTDHVRQQKLP"
     gene            407..1543
                     /locus_tag="VF089_13075"
     CDS             407..1543
                     /locus_tag="VF089_13075"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX6174943.1"
                     /translation="MAPILPGAVVGVLGSGQLGRMFAIAARQLGYRVHTFSPETDTPT
                     GQVADVEITAPYHDLDAIRDFAQNVHVLTFEFENVPALAVDAAAEFAPVRPSGSVLHI
                     AQHRLREKSFLEEKGFPVAPFRAVNSLDELRAAIEQIGCPAILKTAVSGYDGKGQVKL
                     TNPADAETAFTATGSQEKILEAFIDFELEFSIVAARGYDGTFSHWGLIENTHRNHILD
                     LSFAPARVDRETSVQAVEIARTLLETLDVIGVLCIEFFLSRDRRVFINELAPRPHNSG
                     HLTINAALTSQFEQQLRAVCGLPLGATDFLSAAAMANLLGDLWQDGEPNWAAAAGCPD
                     VKLHLYGKDQVRPGRKMGHLTALAPSANAAVERVLDARKRLSKR"
     gene            complement(1677..2402)
                     /locus_tag="VF089_13080"
     CDS             complement(1677..2402)
                     /locus_tag="VF089_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174944.1"
                     /translation="MIEIPKAKQAATVILLRPGKSLGMEVLLTRRPVEMAFLGGMYCF
                     PGGTVQKEDFSSAMLRRCHGLSPAAARKIIGAHFSPQEALGLWVAAIRELFEEVGILL
                     ATTADGTVATMDRELSRSLAETHSSLVSQHSSFRALLEAENLQCDTTRLAYFSHWRTP
                     SQFKVRFDTRFFLASVPAEHSPLTTSPEVADSLWLTPDRALQRFAHGTLPMIFPTFAS
                     LRILADFDSLESLFAEFRPAKSD"
     gene            complement(2399..3163)
                     /locus_tag="VF089_13085"
     CDS             complement(2399..3163)
                     /locus_tag="VF089_13085"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX6174945.1"
                     /translation="MCTLALYFKVFPGYPLAVAANRDEHYDRPSAPPSIIGRSPTIVA
                     GRDLRAGGTWLGINEYGLVVGILNRRQQPGLLTTRAYRSRGLLCLELLRLRQVAELEQ
                     VFLNLAPHDYQPFTVVCADTDRACVAYNTEQTTERIGLFDGLHVFSNSFEFDAPSEKV
                     DRIYQRFRNLSQAGDRPLNIGDYLEGAAKALGEHALGDQSNDSGDAVCVHGDISGTVS
                     SSIILFSDAEREFQMHYCAGTPCRNPFSLSATLNVR"
     gene            3270..4229
                     /locus_tag="VF089_13090"
     CDS             3270..4229
                     /locus_tag="VF089_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174946.1"
                     /translation="MFQSVDQVIAGFGAQNYICNKNLATVVYLGICLQKPILVEGPAG
                     VGKTELGKVLAGSLGLELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKEKIGEVLQE
                     AKTLQQAVERIARQDGVFFSDRFLLPRPLLRAILSHKRSVLLIDEVDKSDAEFEAFLL
                     EVLSDFQISVPEIGTLKAKHVPLVVLTSNSSREMSDALKRRCLHLHIDYPDAQRETEI
                     VNLKVPGVDQKLTENVIQLIQRIRKLDLKKTPSISETLDWVKALTLLNVKVLDDELVN
                     ETLSVLVKYEADIRKAQQELQSYLAERRTMQGARPQDDKDHLH"
     gene            4243..>5597
                     /locus_tag="VF089_13095"
     CDS             4243..>5597
                     /locus_tag="VF089_13095"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6174947.1"
                     /translation="MQDQIIEFANVLRRNGVRVSLSENIDAFRAIDLLGIENAPQFRA
                     ALRTTLVKRAVDLRAFEELFDLFFLGIGQSLNQAEQQLMEQLGLSPDEFQKLLERIER
                     FLKKMEGELSDLTRALLRGNKGELERLLRQAAAAEAERGSKQGFRFTPFTKIASNLEL
                     DKLQDEIERLKGHLQMLAQSGEDSEKIIRYLDQRLKDLYAMLREIIHQEQRKRGIDGS
                     DRNRLVTLTEKSFSFYSEDDIQRMNEAVTRLAQRFKNRLSVRRKNAARGRFDLNETLR
                     KNLQFGGVPFNIQLDRRRKTKPQVVVLCDISDSVLNASRFMLQFVYSIQDLYSKVRSF
                     VFVSDIGEVTQLFEEHDIHKAIEMALTGNIIDVYSHSNFGRAFELFYKEYFSVVTNKT
                     TVLIIGDGRNNYNRPSEWVLREIQQKAKQLIWLNPESRMTWGVGDSEMPRYAPYCKVA
                     E"
BASE COUNT         1368 a         1447 c         1515 g         1267 t
ORIGIN      
        1 cagtgcctca cgagaatttg attgtgtcgg cgcaccgcac tccggcttgg ctagcggagt
       61 ttgccggcac cgctgaagcg cgaggcatcg aagtgatcat tgccggcgcc ggtggcgcgg
      121 cgcaccttcc cgggatggcc gccgctcaaa ccctcgttcc cgttctcggt gtcccggtgg
      181 agagcgcggc cctcaaagga atggattcac tactgtcgat tgtgcagatg ccgggaggta
      241 ttccagtcgg gacattggcc atcggaaaag ccggcgccat aaatgcggcc ttactggcca
      301 tcgctatcct cggcaattca cgggctgagt tgcgcgagaa gctgcggcaa tctcgcgcaa
      361 ggcaaaccga ccacgttcgc cagcaaaaac tgccgtgaga gattctttgg ctccgatctt
      421 gccaggtgcc gtcgtcggcg ttctcggtag cggtcaatta ggaagaatgt ttgccattgc
      481 tgcccggcag ctaggttacc gcgtccacac gttttctccg gaaacggata cccccaccgg
      541 tcaggtagcc gacgttgaaa tcaccgcgcc ttatcacgat ctcgatgcaa tcagggactt
      601 tgctcaaaac gttcacgtgc tgacattcga gtttgaaaat gtccccgcgc ttgcagtcga
      661 cgctgcggca gaattcgccc cggtacgacc gtccggctca gtactgcaca tcgcacaaca
      721 tcggctccgc gagaaatctt ttcttgaaga aaagggcttc ccggtggctc ctttcagagc
      781 ggtgaattcc ctcgatgagc tgcgcgccgc catcgagcaa atcggctgtc cggcaatttt
      841 aaaaacggca gtttccggat acgacggcaa ggggcaggta aaactgacca atcccgccga
      901 tgccgaaacg gcttttaccg caacgggcag tcaggagaag atccttgaag cctttatcga
      961 tttcgaattg gagttttcaa ttgtcgctgc acgcggttac gacgggactt tttcacactg
     1021 gggcctgatc gagaacaccc atcggaatca tattttggac ttatcatttg caccggcacg
     1081 tgtggatcga gagaccagcg ttcaagctgt ggagattgct agaactctgc ttgagacact
     1141 agatgtcata ggcgtcctat gtatcgaatt ctttcttagt cgcgaccgaa gagttttcat
     1201 caacgagctc gcgccacgcc ctcataattc agggcatcta acgatcaacg cggccctgac
     1261 cagccagttt gagcagcagc tgcgcgcggt gtgcggcttg cctctaggag caacggattt
     1321 tctgtcggcg gcggcgatgg ccaatctctt gggcgatctc tggcaagacg gagagcccaa
     1381 ttgggccgcc gctgcgggtt gcccggatgt aaaattgcat ctctatggca aagaccaagt
     1441 tcgtccaggc cggaaaatgg gtcacttaac tgctctagcg ccgagtgcaa atgcagccgt
     1501 ggagcgcgtt ctggacgctc gcaaacggct cagcaaaaga tgacgtcttc cacaatcgag
     1561 ttcctgtgag gctttcacag gcggctgaga taccgtgacg attttcctgc cgcgactact
     1621 tcgcctagtc catcatacgg attgctctag ttgctgtttg actgatctgc tgtgagctag
     1681 tcactctttg ccggtctgaa ttctgcaaac aggctctcca aagaatcgaa gtcggctagt
     1741 attcttaacg atgcaaatgt cggaaagatc atcggcagag tgccgtgggc aaatcgctgc
     1801 agagcacgat cgggggtaag ccaaagacta tcggcaactt caggagacgt tgtcagaggc
     1861 gaatgttccg ctggaacgga ggcgaggaaa aaacgtgtgt cgaagcgcac cttgaactgt
     1921 gatggtgtcc gccagtgtga gaaataagcc aatcgagtag tgtcacactg taagttctcc
     1981 gcttcaagca acgccctgaa actcgagtgt tgagaaacca acgagctgtg tgtttccgca
     2041 agcgagcgac ttagctcgcg atccatcgtc gccactgtcc catctgccgt agtagctagc
     2101 aaaattccaa cctcttcgaa tagctcccgg atggccgcga cccatagccc cagcgcttct
     2161 tgaggactaa agtgagcacc gatgattttc cgggcggcag cgggagagag gccgtgacac
     2221 cggcgcagca tcgcgctcga aaagtcttcc ttttgcactg tgccaccggg gaagcagtac
     2281 attccaccaa gaaaggccat ttcgacaggt cgacgggtca gcagcacttc cataccgagg
     2341 gactttcctg gccgaagtag gatgaccgtg gcagcttgct tcgctttggg gatttcgatc
     2401 atcgcacgtt caaagtcgcg gataggctga aaggatttcg acaaggtgtg ccggcgcagt
     2461 aatgcatttg gaactctcgc tcggcatcgg agaacaaaat gatgctggaa gaaacggttc
     2521 cggagatatc gccgtgaaca caaactgcat ccccggaatc atttgactga tcgccaagcg
     2581 cgtgctcgcc gagcgcttta gctgcgcctt cgagataatc tccgatgttg agtgggcgat
     2641 ctccggcctg ggacagattg cggaaacgtt ggtagatccg atcaaccttt tcagagggcg
     2701 catcgaattc gaagctgttg ctgaacacgt gaagcccatc gaaaaggccg atccgttcag
     2761 tagtttgctc ggtgttgtaa gcgacgcacg cgcgatcggt atcggcacag accaccgtga
     2821 acggctgata gtcgtgtggc gccagattca gaaacacctg ctcgagctca gcgacttgcc
     2881 ttagacgaag aagctccaga cacaacaagc cgcgcgagcg gtatgcacgg gttgtgagta
     2941 aacccggctg ttgccggcga ttgagaatgc ccacgaccaa accatattca ttgattccta
     3001 accaggtgcc gccggcacgc aaatcccggc cggcaacaat cgttggcgac cgccctatga
     3061 tcgaaggcgg cgccgaaggc cggtcatagt gctcgtcccg gttggccgcg acagccaagg
     3121 ggtaacccgg aaaaactttg aaatagagcg ctaaagtaca catgctgagt gagttacaga
     3181 agcctaggaa atataacatg aaacttcaaa cagcgagatt tgttgtcata tcgcaattgt
     3241 gttagcgttc tgaggattga gaggtcttca tgtttcaatc tgtggatcag gttatcgctg
     3301 gttttggcgc tcaaaactat atttgtaaca aaaatttagc caccgtcgtc tatcttggca
     3361 tttgcctgca gaagccgatt ctggtcgaag gaccagccgg tgtgggtaag accgaacttg
     3421 gcaaagtatt ggcaggttca ttgggattgg agctcatccg attgcaatgc tacgaaggct
     3481 tagatgaagc gaaggcgctc tacgaatggg aatacgcaaa gcagttactt tacacgcaaa
     3541 ttcttaagga aaagatcggc gaagtcttgc aggaagctaa aactctgcaa caggccgtgg
     3601 agcgcattgc gcgccaggac ggtgtcttct tttctgatcg tttcttgctg ccacgcccgt
     3661 tgctgcgcgc cattttatcg cataagcgga gcgttctact gatcgacgaa gtggataagt
     3721 ctgacgctga atttgaggcc ttcttgctcg aggttttgag cgatttccag atcagtgtgc
     3781 ccgagatcgg cactctgaag gccaaacacg ttccgctcgt ggtgctcacc agtaacagca
     3841 gccgtgagat gtccgacgct cttaaacgcc ggtgtctgca ccttcatatc gattaccctg
     3901 atgcccagcg cgaaacggag atcgtcaacc tcaaagttcc tggagtcgat caaaaactga
     3961 cggagaatgt cattcagctg atccaacgta ttcgcaagct cgacctgaaa aaaactccaa
     4021 gtatcagcga gactctcgat tgggtaaaag ctttaacttt gctaaatgtc aaagtgctcg
     4081 acgacgaact ggtgaatgaa actctatcag ttcttgttaa atatgaagcc gatatccgaa
     4141 aggcgcaaca agaactacaa agctatctgg cggagcgccg gactatgcaa ggcgccagac
     4201 cccaagatga caaggatcat ctgcattaaa taggtttttc cattgcagga ccagatcatc
     4261 gaattcgcca acgtcctcag gcgcaatggc gtgcgggtgt cattgtcgga gaatatcgac
     4321 gcatttcgcg ccatcgattt gctgggaatc gaaaatgcgc cacagtttcg cgccgccctc
     4381 cgaacgacgc tggtaaaacg cgcggttgat ctgcgggcgt tcgaagaact attcgatctt
     4441 ttctttttgg gtatcggaca atctctcaac caagcggaac aacagctgat ggagcagctt
     4501 ggtctctcac ccgacgagtt tcaaaaactg ctcgaacgga tcgagaggtt cctcaagaaa
     4561 atggaaggcg agctatccga tctcacccgg gctctgcttc gcggcaacaa aggagagctg
     4621 gagcgcttgt tgcgccaggc agctgccgcc gaagcggaac gaggctcaaa acaaggtttt
     4681 cgcttcacac ctttcactaa aatcgcgtcc aacctcgaac tggataagct tcaggatgaa
     4741 atcgaacggc tcaagggcca tttacaaatg ctggcgcaaa gcggcgaaga ctcggaaaag
     4801 attattcgct atcttgacca gcgtcttaag gatttgtacg caatgctccg tgagatcatt
     4861 caccaggagc agcgaaagcg cggaatagac gggtcggatc gaaatcggtt ggtgacactg
     4921 actgaaaaga gcttcagctt ctatagcgaa gatgacattc aacggatgaa cgaggcggtg
     4981 acccggctgg cgcagcgatt caaaaataga ctctctgtca ggcgcaagaa cgctgctcgc
     5041 gggcgcttcg atttaaacga gacactgcgc aaaaatcttc aatttggcgg agttccattt
     5101 aatattcaac tggaccgccg cagaaaaacc aaacctcagg tcgtggtgtt gtgcgacatt
     5161 tccgattctg tcttgaacgc atcgcgcttc atgcttcaat ttgtttattc cattcaggac
     5221 ctctattcga aggtgcgaag ttttgtcttt gtcagcgaca ttggcgaggt gactcagctt
     5281 tttgaagaac acgatatcca taaggccatt gaaatggctc taacgggcaa tatcatcgat
     5341 gtgtactcgc actcaaactt tggccgcgcc tttgagctgt tctacaagga atatttttct
     5401 gtcgttacca ataagaccac ggttttgatc atcggtgacg gccgtaacaa ttataaccgt
     5461 cccagcgaat gggttttgcg agaaattcag cagaaggcca agcaactgat ttggctcaat
     5521 cctgaaagta ggatgacctg gggagtagga gatagtgaaa tgccgcgtta cgcgccttac
     5581 tgcaaggttg cggagga
//
LOCUS       DASUTG010000567         7439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1273156, whole genome shotgun sequence.
ACCESSION   DASUTG010000567 DASUTG010000000
VERSION     DASUTG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273156"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VF089_13100"
     CDS             complement(<1..1340)
                     /locus_tag="VF089_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174948.1"
                     /translation="MKVNRRLVILICLWLLAAFGFFLFRSSAPLLVWSDSPELSANLY
                     GSFGLGPRQTLKQKGLLEQIIRESFQSSTTTGYRPVNFIIHKLGVAFFSTPEASPYLW
                     FAAVSLVCGTLAVCFFLVARRYTRTDFGAIVAVTLLLCSPSVVGSGWLIFSGIHALVL
                     LLICLGLLLYWKILERPHNAPWYLAGLCAVLLLGPWYREFIGGLSVLIMFLECQRARR
                     PTPLVFLAGLFLLHAVFPTAIIRLIAFPDLPLRPVFALGQLGVQVQLASAPADQSVIS
                     HLINSIRWRALFSFLALFPPTLVGLSLIAYVLPSSWRQHSISFPATRYGGGYLFLGFW
                     LLVFFLPFLRIFTLHAHLAYPLMPFSIIVAVGAERLWQMTSHRTRLSRILRNVLAVVL
                     GVSVCDHLLNVYGSYRTVDAINSGSLAVADWFQKQVPAGSIVISNALHIEDIRL"
     gene            complement(1500..2279)
                     /locus_tag="VF089_13105"
     CDS             complement(1500..2279)
                     /locus_tag="VF089_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174949.1"
                     /translation="MISFLLAHPLTRGLDLDDPNTTELRRQIIREKSFLRQIYQEWYT
                     AIASKISSREGPVLELGSGAGFLKDFVPGLITSEVFHCRGIDIVLNGIELPFADGALQ
                     AIVMIDVFHHIPQPRRFFTEAARCVRPGGVVLMIEPWVSVWSQLVYTRLHHEPFQPEA
                     KQWEFPNSGPLSGANGALPYIVFARDRIQFEREFPMWQIQRITPMMPFCYLVSGGVSM
                     RSLMPGWTFKSWRAVENSLSPFINKLAMFACIVLQKQECEP"
     gene            complement(2294..3796)
                     /locus_tag="VF089_13110"
     CDS             complement(2294..3796)
                     /locus_tag="VF089_13110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009149464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6174950.1"
                     /translation="MMPELVHVNEEALDLSSAVEHADVSARGYQKARMAHWDQVARSI
                     AAGWNGSGAFYQKQLAHYYSLLISPGRRVLEIGCGTGDLLAAVNPSVGVGVDFSREMI
                     KQAREKYPQLSFIEADAHEVAFNGKFDVIVLSDLVNDLWDVQSVFKQLRSVSHKRTRL
                     ILNTYSYVWQPLIAFSQLLQLSKPTLQQNWLTVEDLANLLSLADFDVIKRMREILCPV
                     PLPLAANFANRFLVKLWPFSMLAMTNFVIARPQNSIAIRTADEQPRVSIIVPARNESG
                     NVDRIFVRTPALGATTELVFIEGHSRDDTYAAIERAMVKYSNRACRLMKQLGEGKGDA
                     VRLGFDQAKGDILMILDADLTVAPEDLVRFYDLLVSGKADFVNGVRLVYPMEDQAMRF
                     VNLIGNKFFSLAFSWLLSQSIKDTLCGTKVLWKKDYELIKANRAYFGDFDPFGDFDLL
                     FGAAKLNLKIADMPIRYRERTYGTTNIQRWKHAWLLFKMVRFAAGRIKFV"
     gene            complement(4019..4723)
                     /locus_tag="VF089_13115"
     CDS             complement(4019..4723)
                     /locus_tag="VF089_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6174951.1"
                     /translation="MKVTLLVLALNEIDGMKAIMPRVKKEWYDQIIILDGGSTDGTIE
                     YAKEQGYFVYVQKRRGFRHAYTEVLPYVEGDVIVTFSPDGNSVPERIPALVEKMSEGY
                     DMVIGSRYLDGAKSEDDDVITAFGNWLFTKTINLLHGGCYTDAMVIFRAYKTQMIYDL
                     ELHTDEPYAAAEKIFNTTISWEPLLSVRAAKRKLKIAEIPADEPPRIGGERKLRVIQW
                     GGAYYFQFLREKLFWP"
     gene            complement(4720..5313)
                     /locus_tag="VF089_13120"
     CDS             complement(4720..5313)
                     /locus_tag="VF089_13120"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6174952.1"
                     /translation="MGAKPQQPELTNRKLVCENSQFHIFFDELSLQGRSSIRDYLVVA
                     PKRVAADLVTGVAILPIVDGKIGLIRIYRHPIGRYSWEIPRGFIDQQEVSSSAAGREL
                     KEETGLDCKSAAIRSLGFITPDGGILAARVQLFAAMKCAQEREYAPAEIGHRELRFFN
                     MQQVEEMIFRSEVQDPSTVIAFFMYRRFENQVLDGPV"
     gene            complement(5367..6881)
                     /locus_tag="VF089_13125"
     CDS             complement(5367..6881)
                     /locus_tag="VF089_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX6174953.1"
                     /translation="MSHNGQLDLVLVHPGTRMQVYQSLGSKLAAIEPPVWASLLATFV
                     RRHGFSVQIVDAEAENLTPEETAMRIEEMNPKLAVIVVYGHQPSASTQNMTVSGLICA
                     ALKELNPDRKLALVGGHVAALPERTLREESADFVCTGEGPYTVVDLLQAANSISPDYS
                     KVRGICYWKDGIVTSTPAAPLVKNLDDEIPELAWDLLPMGKYRAHNWHCFGDLQREPY
                     ASFYTTFGCPFHCSFCCIQAPFKTGEKEAGWKDSVNSYRFWSAQAVIAQIDKLVNQYG
                     IRNIKIADEMFVLNSRHILGICDAIIERGYDLNIWAYARVDTVKDGMLEKLKRAGFNW
                     LAFGIEAASDRVRDDVDKGFDQEEIFRTLEKVRGTDINVIANYIFGLPEDDLESMQST
                     LNLALELNCEFANFYSAMAYPGSQLYNLAIKEDWPLPEKWSGYSQHAVDTLPLPTKYL
                     SGAEVLRFRDEAFHRYFSSPQYLRMIEAKYGSLTAEHIREMASQRLVRKILAAA"
     gene            complement(6871..>7439)
                     /locus_tag="VF089_13130"
     CDS             complement(6871..>7439)
                     /locus_tag="VF089_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6174954.1"
                     /translation="SHGMVRESSSSDLKETFYRNYPDLNPDFIFAFPAYNVRSTEINA
                     VIGRSQLKRLDANNRIRSENLKLFLEHLDPEKYQTDFVTTGSCNYAFTLVLNRADPVL
                     CENVMQTMRRYNVEFRRGTSGGGNQLRQPYLRKIMGEREYENYPNVDHVHFYGFYIGN
                     YPDLEREKIQQLCSLLNSISSEEARYVS"
BASE COUNT         1942 a         1959 c         1793 g         1745 t
ORIGIN      
        1 aagagccgta tgtcttcgat gtgcaatgca ttacttatta cgatcgaacc cgcgggtacc
       61 tgtttctgaa accaatctgc cacagccaaa ctacccgaat taatggcatc gacagtgcga
      121 tagcttccat aaacattcag aagatgatca cagacagaaa ctcccaatac cactgctagg
      181 acattgcgga gaatgcgact taagcgagtc cgatgtgatg tcatctgcca gagccgttca
      241 gctccgacgg ctacaatgat tgaaaatggc atgagcgggt aggctagatg cgcatgaagt
      301 gtgaatatcc gcagaaacgg tagaaagaat accaaaagcc aaaaacccaa aaagagatag
      361 cctccaccat atctcgtcgc aggaaaactg atactatgct gacgccagct gcttggaagc
      421 acataggcaa tcagagacag tccaaccaaa gtcggcggaa acaacgcaag aaaactaaag
      481 agcgcgcgcc accttataga attgattaaa tgacttatca ctgactggtc tgcaggagct
      541 gaggcaagtt ggacttgtac acccaattgc cccaaggcga atactggccg caatgggaga
      601 tcaggaaaag cgattagcct gataattgct gtcgggaaga cagcatgcaa taagaataac
      661 cccgccaaaa acacgagcgg cgtaggacgg cgtgcccgtt ggcattccag aaacattatc
      721 agaacgctca aaccaccgat aaactcgcga taccaggggc caagcaaaag caccgcacat
      781 aggccagcca aataccaagg agcattgtgg ggcctctcga gtattttcca ataaagtaac
      841 agtcctaaac atatcagcaa caaaacaagg gcatgaatcc cggaaaagat cagccagccg
      901 ctgccaacga ctgagggaga acagagtaaa agagtgaccg cgacaatagc gccaaaatcc
      961 gtccgtgtgt aacgacgcgc gaccaaaaag aagcaaaccg ccaacgtacc acacactaga
     1021 cttaccgccg caaaccagag atatggcgaa gcttcgggtg tactaaaaaa cgctactccg
     1081 agcttgtgaa tgatgaaatt gactggccga tagccagtcg ttgtgctgct ctgaaaagac
     1141 tcgcgaatga tctgttcaag cagtcctttt tgcttcaatg tctgacgtgg accaagacca
     1201 aaagagccat acagattcgc gctcaactcg ggggaatcgg accatacgag caacggtgca
     1261 gacgatcgaa agaggaagaa gccgaaggca gccagtagcc agaggcaaat gaggattact
     1321 aaacgccgat taactttcat aaactctatg gattctctag tcttgccctt ggtaggctat
     1381 tgtagttgct tcattcgtcc gtgcacgagc gccataaggg cgctagactt gcgcttggaa
     1441 gttgtattgt ttggcgcgag tagcggcgtg gattccggat gctcaggtat ggcttgaagt
     1501 tatggttcgc attcttgttt ttgcagcaca atgcatgcaa acatcgcgag tttgttgatg
     1561 aacggcgaca acgagttttc taccgcgcgc caggacttga aagtccaacc tggcatcagg
     1621 ctgcgcatcg agacgccacc ggaaaccaaa taacaaaacg gcatcatcgg cgtgattctc
     1681 tggatttgcc acatgggaaa ctcacgctcg aactgtattc gatctctggc aaagacaata
     1741 tacggcaacg ctccattagc gccggatagt gggccactgt ttggaaattc ccactgcttt
     1801 gcttctggct ggaatggttc atggtgcaac cgcgtgtaaa caagctgaga ccaaactgaa
     1861 acccacggct caatcatcaa aacgacacca cccggccgaa cacaccgcgc agcttcagtg
     1921 aaaaagcggc gcggttgcgg tatatgatgg aacacgtcga tcatgacaat tgcctgcaaa
     1981 gcaccgtcgg caaagggtaa ctcaatccca tttaagacta tatcgattcc acgacagtga
     2041 aaaacctcgg acgtaattag gccaggtacg aaatccttca gaaagccggc ccctgatcct
     2101 aactccaaca ccggtccctc tctgcttgag atcttactag ctattgctgt gtaccattct
     2161 tgataaatct gcctgagaaa gcttttttct cggatgatct gccgacgtaa ttccgtcgta
     2221 ttgggatcgt caaggtccaa ccctctcgtc aaaggatgcg cgagcaagaa actgatcatc
     2281 agctcactcg gaatcagaca aatttgattc ggccagcagc aaaccgtacc atcttaaaaa
     2341 gtagccaggc atgcttccac cgttggatgt tagttgtgcc gtaggtacgc tctcgatagc
     2401 gaattggcat atcggcgatt ttgagattga gtttggccgc gccaaacaga aggtcgaaat
     2461 cgccaaatgg gtcaaaatcg ccgaaataag cacggttggc tttaatcaat tcgtaatcct
     2521 ttttccataa cactttcgta ccgcacagag tgtcttttat ggattggctg agcagccagg
     2581 agaaggcaag gctaaaaaat ttgttcccga taaggtttac gaaccgcatc gcttggtctt
     2641 ccattggata caccaatcga acgccgttaa cgaaatctgc tttgccggat acgagcaggt
     2701 cgtagaaacg aaccaaatct tcaggagcca ccgtcaggtc agcgtcaaga atcatcaaga
     2761 tgtctccctt cgcctgatca aagcctaatc taacagcatc cccctttcct tctcctaact
     2821 gtttcatcaa tctgcacgcc cgattggaat atttcaccat tgcccgttct atcgctgcgt
     2881 aagtatcatc tctggaatgt ccctcgataa agacaagctc cgtggtggca ccgagcgccg
     2941 gtgtccgaac aaaaatgcgg tcaacgttac cgctctcgtt tcgggctgga acaattatgg
     3001 agacgcgtgg ctgttcatca gcggttctga ttgcgattga attttggggc cgagcaataa
     3061 caaaattagt catcgctaac atagaaaatg gccatagttt gaccaaaaac cggttggcga
     3121 aattagcggc aagcggcaaa ggaacggggc acaatatctc ccgcatacgt ttgatgacat
     3181 cgaaatccgc caagctgagc aaattggcaa gatcttccac ggttagccaa ttttgttgga
     3241 gggtgggctt gctcaactgc agtagttgcg aaaatgcgat cagcggctgc caaacatagc
     3301 tgtatgtatt caggattaag cgcgtgcgtt tgtgactgac cgagcgcaat tgtttaaaga
     3361 cgctttgaac gtcccacaaa tcatttacta aatccgaaag cacaatgaca tcgaatttgc
     3421 cattaaaggc gacttcgtgt gcgtcagctt ctataaagct tagctgcgga tatttctccc
     3481 ttgcctgttt aatcatttct ctcgaaaaat ccaccccgac accgacagat gggttcactg
     3541 cggccagtag gtcgcctgtg ccacagccga tttctagcac tcgccttccg ggtgagatca
     3601 aaagtgaata gtagtgagcg agttgtttct ggtaaaaggc tcccgagccg ttccaaccgg
     3661 cggcaatgct tctagccacc tgatcccagt gagccattcg agctttctgg taaccacgcg
     3721 cacttacgtc cgcgtgctca acagccgaag atagatccag cgcttcttcg ttcacatgga
     3781 ctaattctgg catcatctag agcgctcctc agatcgcggt gataaggtta gctagcccgt
     3841 gcagcatctt gcccaccaaa ataaagtggg gcatggcgca agcgcgaacg agactctgaa
     3901 aacgactttt ttcaaatgta gcaacaggat ccaagtaggt catcgtaaac cgagtcaact
     3961 caattcgtca cgagttgcaa agtccgccga tacagcatca aactaaggcc aaaacaaact
     4021 aaggccaaaa caacttctcc cgtaggaatt gaaaatagta cgctccaccc cattgtatga
     4081 ctcgaagctt tctttcgccg cctatccgcg gtggttcgtc ggccggaatc tcggctatct
     4141 tgagttttcg cttagccgcc ctgaccgaca gcagcggctc ccagctgatc gtcgtattga
     4201 aaatcttttc agcggcggcg tagggctcat cggtatggag ttcgagatcg tagatcattt
     4261 gagttttgta agcgcggaag atcaccattg catctgtata acatccgcca tggaggagat
     4321 tgatcgtttt agtgaacagc cagttgccga aggccgtaat cacgtcatca tcctcgctct
     4381 tggccccgtc caggtaacgg gagccaatca ccatgtcgta tccttcgctc attttctcga
     4441 ctaatgcagg aatgcgctcc ggaaccgagt ttccgtcggg gctgaaggtc acgatcacgt
     4501 ccccttcaac gtacggcaat acttcggtat aggcgtggcg aaatccgcgc cttttctgga
     4561 cgtagacgaa gtatccctgc tctttagcat actcgatcgt tccatcggtc gagccgccat
     4621 ctaaaatgat gatctggtca taccattctt tcttcacccg cggcatgatc gccttcatac
     4681 cgtcgatttc attgagagca agaaccaaca gagtcacttt cacactgggc cgtccaaaac
     4741 ctggttctca aatcgcctgt acatgaagaa tgctatgact gttgacggat cctgcacctc
     4801 agaacgaaaa atcatttctt cgacctgctg catattgaag aaccggagct ctcgatgacc
     4861 gatctccgcg ggtgcatact cccgttcctg tgcgcatttc atcgccgcaa acagctgcac
     4921 tctggccgcc aaaattcctc cgtcgggagt gataaagccc aacgaccgaa tcgccgcgga
     4981 cttgcaatcg agtccagtct cttcttttag ttctcgaccc gctgcgctcg aagacacctc
     5041 ctgctgatcg atgaaacccc gcggaatttc ccatgaatag cgcccgatgg gatgacgata
     5101 aatccggatc aggccgatct tgccgtcaac gatcggcaga atcgccactc cagtgacgag
     5161 atcagcggcg acgcgttttg gcgcaaccac cagataatct ctgatgctgc tgcgcccttg
     5221 gagactcagt tcgtcaaaga agatatgaaa ctggctgttc tcgcagacaa gctttcggtt
     5281 tgttagttcc ggctgctgag gtttcgcgcc catcggctca gcagatcttc gctttcgcac
     5341 agaaacgcgt cgcacgcagt gatcgttcag gctgcagcca agatttttct gactagccgt
     5401 tgtgaagcca tttcgcgaat gtgctcagcg gtgagcgaac cgtactttgc ctctatcatc
     5461 cgcaaatact gtggactgct gaaataacgg tgaaaagcct catcgcgaaa cctcagcacc
     5521 tccgcccctg ataaatactt cgtgggcagc ggcaaggtgt cgacggcgtg ctgcgaatac
     5581 ccggaccatt tctctggaag gggccaatct tccttgatcg cgaggttgta taattgggat
     5641 ccaggatagg ccatggcaga atagaagttg gcaaattcac agttgagttc gagcgctaaa
     5701 ttgagcgtcg actgcatgct ttccagatca tcttccggaa gaccaaaaat atagttggcg
     5761 atcacgttga tgtctgttcc tctgaccttt tccaaagtgc gaaagatctc ctcctggtcg
     5821 aaccctttat ccacgtcatc gcgcacccga tcactggcgg cctcgatgcc aaacgccagc
     5881 cagttaaagc ctgcgcgttt gagcttttcc agcattccgt ccttgaccgt gtcgacgcgg
     5941 gcataagccc agatattcaa gtcgtaaccg cgttcgatga tcgcgtcgca gatccccagt
     6001 atgtgacgtg aattcagaac gaacatctcg tccgcgattt tgatattacg aatgccatac
     6061 tgatttacaa gcttatcgat ctgagcgatc accgcttgcg cgctccagaa gcgatagcta
     6121 ttgacactgt ctttccatcc cgcttccttt tctccggtct taaacggggc ttggatacag
     6181 cagaaactgc aatgaaaagg acaaccgaac gtagtgtaga acgacgcata gggctctcgt
     6241 tgcagatcgc cgaaacagtg ccagttgtgg gctcggtact tgcccatggg caaaaggtcc
     6301 catgcgagtt ccgggatttc gtcatccaaa tttttgacca gcggagctgc gggtgtggac
     6361 gtgacaatgc cgtccttcca gtaacagatt ccccgcacct tcgaatagtc cggcgatata
     6421 gagtttgcgg cttgcaacaa gtcgacgacc gtgtacggtc cttctccggt gcacacgaaa
     6481 tccgccgatt cctctcgtaa cgtacgttcc ggaagggccg ccacatgtcc gcccacaaga
     6541 gctaatttgc gatcggggtt gagttcttta agcgcagcgc agatcaagcc tgagaccgtc
     6601 atgttttggg tggaggcaga tggttgatgt ccgtaaacga caatcaccgc cagtttagga
     6661 ttcatctcct cgatccgcat cgcggtctct tcgggtgtta gattctccgc ttcggcatcg
     6721 acgatttgaa ccgagaaacc gtgtctacga acaaaggttg ccagcagact tgcccatacc
     6781 ggaggctcga tcgctgccag cttcgatccc agtgattgat aaacctgcat ccgggtaccg
     6841 ggatgaacca gcaccaggtc caattggcca ttatgagaca tatcgtgctt cctcactgga
     6901 gatgctgttt agcagagaac ataactgctg gatcttttct cgctcaagat cgggataatt
     6961 tcctatgtag aagccgtaga agtgaacatg atcgacattt ggataatttt catactcgcg
     7021 ctcgcccata atttttctca gatacggctg cctcaattga tttccgccgc ccgaggtgcc
     7081 gcggcggaat tctacgttgt agcgccgcat cgtttgcatc acgttttcgc acaacaccgg
     7141 atcagcgcga ttgagcacca acgtgaacgc ataattgcag cttcccgtcg tgacaaaatc
     7201 ggtctggtac ttttcaggat ccaggtgttc aaggaacagt tttaagttct ccgatctgat
     7261 tctgttgttg gcatcgagcc gcttgagttg agaacggccg atcacagcat ttatttccgt
     7321 gcttcgaaca ttgtacgccg ggaaagcaaa tatgaagtcg gggttcagat ccggatagtt
     7381 tcgataaaag gtttccttga gatcggacga actcgactcc cgcaccattc catgggaac
//
LOCUS       DASUTG010000568         5725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1125101, whole genome shotgun sequence.
ACCESSION   DASUTG010000568 DASUTG010000000
VERSION     DASUTG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125101"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF089_13135"
     CDS             complement(<1..238)
                     /locus_tag="VF089_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX6174955.1"
                     /translation="MSPSEDPGLEIDSADLHQNAKVNEPDGHSPFPIVGIGASAGGLE
                     AFQQLLGQLPADINMAFLLVQHLDATHESHLTHIL"
     gene            complement(513..1448)
                     /locus_tag="VF089_13140"
     CDS             complement(513..1448)
                     /locus_tag="VF089_13140"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX6174956.1"
                     /translation="MHNLGSYFSKWIDSERNVLAPAIHASDVDRINWFRCLPFLLLHV
                     ACIAVWWVGWSGFAVCTAVGLYVVRMFAITGIYHRYFSHRTYRTSRWIQFVFALLGAS
                     SAQRGPLWWAAHHRSHHRFSDMQPDPHSPIRHGFWRSHMGWFMTNRHYLTDYRRIHDF
                     ARFPELRWLNRFDLIVPIILAVTLYFVGRVLSYAAPALGVTGWQLVVWGFVVSTIMLF
                     HATASINSLAHLIGRRRYDTGDDSRNNFILALITLGEGWHNNHHKFRSCTRQGFYWWE
                     IDLTYYGLKFLAAVGLIWDLRPVPGKAYDHNQQLS"
     gene            2225..3349
                     /locus_tag="VF089_13145"
     CDS             2225..3349
                     /locus_tag="VF089_13145"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX6174957.1"
                     /translation="MPNVVGLFDSRSEAHATVQQLVNSGFDREDISLVSQDTDRQPND
                     GKTHPGISGSDLSIPKDDTSGALKGAGIGAALGGIGGLLAGIAGLAIPVIGPIVAAGP
                     IAAALTAALGGAGVGAATGGLIGALTDMGVPEDEAKHYQDQVGRGKTLVIVRVSDDAS
                     ADRAADILESSGAEEIDGRETDTDYILDRPSAVGGETQSRDVEVSERQALRDDRSEAN
                     PGERDINYRTARIDESGVRAGQESFSPEDRVIPVTEEQLVVGKRAVQSGRVRIYGRVT
                     EKPVEQSVQLQNERVTVDRRAVDRPVRAGDKEDDVVIEITETKEEPVIGKQERVVEEI
                     VIGKTTEEHTETVRDTVRRKDVEVERTAHEERLREEDNRR"
     gene            3740..4267
                     /locus_tag="VF089_13150"
     CDS             3740..4267
                     /locus_tag="VF089_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6174958.1"
                     /translation="MISLDRLDVFDGDELTVIIETPKGSQNKYAYEPRFGTFVLDGVL
                     PAGAVFPFDFGFVPSTIGEDGDPLDVLVLMDAAAFTGCIVASRLIGVIEAEQTENSKT
                     FRNDRLIAVAVKSITHRSLRDISDVSGDLVGQIEHFFISYNMAKGKTFEPKGRSGRER
                     ALALLTTGMARADRA"
     gene            complement(4413..4670)
                     /locus_tag="VF089_13155"
     CDS             complement(4413..4670)
                     /locus_tag="VF089_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174959.1"
                     /translation="MDRTWSVEIEAKKHLIEVEYGRNDSRTGKLMVDGNEAQTWNNSQ
                     WLDLPKEITFEVGGKTAVLRAKGFFKPRIDLFFEEKLIRQA"
     gene            4991..5431
                     /locus_tag="VF089_13160"
     CDS             4991..5431
                     /locus_tag="VF089_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6174960.1"
                     /translation="MALQHPNMTVAHRQLTANERQILEKQVLVHVGIVLPDGTPHVSA
                     MWVDLDGNDIILNTAEGRTKANVLRLGSPVALSVLDPENPYRNLGLRGRVVEVISEER
                     GADAGINRLAKKYLGVEEYPYRAPGERRVQFRVRPEVVSGWGKD"
BASE COUNT         1411 a         1424 c         1572 g         1318 t
ORIGIN      
        1 aaagaatatg cgtgagatgg ctctcgtggg tagcatcgag gtgctggacg agtaaaaaag
       61 ccatgttgat gtcggcgggg agctgaccca acaactgctg aaatgcttca aggccaccgg
      121 ccgatgcacc gatgccgacg atcggaaaag ggctgtggcc gtccggctcg ttgacctttg
      181 cattctggtg gaggtccgca gaatctattt ccagtcctgg gtcttcgctg gggctcatga
      241 ataacttcaa tcctaattgg ctctaacact cagcggtatt ttgtacaacg gaagtaatgg
      301 gtccgtgttc acctacgtga gtcctgccca tatgctgatg caatgcaagt ctcgacttgc
      361 tgtttttcgg ttcccctgct ggtttgatgt tcgctgttta gacttgcgct gggagggaat
      421 ctggcataac gagtcatgac cgtgctctga ttactcgacg tgatcctagt cacttgcccg
      481 cgcctggcaa tcgtttctat ccttggtatt tctcacgaca gctgctggtt gtggtcatag
      541 gctttgccgg gaacgggtct gaggtcccag atgagtccaa cggcggcgag aaacttaaga
      601 ccgtagtaag tcaaatcgat ttcccaccaa taaaatcctt gtcgggtaca actcctaaac
      661 ttatgatggt tgttgtgcca accttcgcct agagtaatca aagcgaggat aaaattattg
      721 cggctatcgt caccggtgtc atagcgacgg cggccgatta gatgcgccag cgaattgatg
      781 ctcgccgtgg catgaaacag cattatcgta ctaaccacga agccccagac aactagctgc
      841 catccggtaa cgcccagggc tggagcggcg taactaagca cacgaccgac gaagtaaagc
      901 gtcactgcaa gaatgatcgg aacgatcaag tcaaaacgat tcaaccagcg caactcgggg
      961 aagcgggcga agtcgtgtat gcggcgatag tctgttaagt aatgccgatt ggtcatgaac
     1021 catcccatgt gtgatcgcca gaaaccatgt cgaatcggcg agtgtggatc gggttgcata
     1081 tcggaaaatc tatgatgact ccgatgatgc gcggcccacc acagcggccc acgttgcgct
     1141 gacgatgcac ctaataacgc aaacacaaac tggatccatc gagacgtccg ataggtgcgg
     1201 tgcgaaaaat aacgatgata gatacctgtg atagcaaaca tgcgtactac ataaaggccc
     1261 acggcggtgc atacggcgaa gccactccat cccacccacc acacggcgat acaagccaca
     1321 tgaagcagca gaaacggtaa gcagcggaac caattgatgc gatctacatc agaggcgtga
     1381 atcgcgggag ccaaaacatt tcgttcagaa tcgatccatt tggaaaaata gctgcctaaa
     1441 ttatgcataa acctttctta aaacgcacgt cctaaaaccc acacacagca acactgccgt
     1501 gatcgttcac ctagttttga tagttcacgt acttaataac ttatgaaaga gcatacggac
     1561 gaccgcaatt gctgtgccag tatcatctca cgtgattctg ttgcgtgcgc tgtgccggta
     1621 acgcaacggt ttgatgtgtt gtgtgtcaga ctaaaccgcg gtcacaaacg agcaaagaat
     1681 tttattcgaa gtcttgcacc acttgattgg gcggtgcgtt cagcacgcct cacttcggct
     1741 tagcatctga cgttcatcgg ttgagccgct accagtatag agcttaagaa caaaacacgc
     1801 tcattgatgg gatgatcagt gaatctcccg tcgcgtcgta ctgcgagagc gattcgagta
     1861 cgggatggtt aagcatccaa cgaattgacg gctgagtttc atttcgtgga gctggcgctt
     1921 caggttgtcc tgaaatcact cgattgtttc gaagtgattc ttaaccctga tacgtttttg
     1981 aggcacttgc gtggcgaaga gtaatcttta aaggcaaacg ctggccacgt tcattggcac
     2041 gtagcttgcg ctaccttctt gatggttagt cctctactcg ataatcactc ccaaatagca
     2101 cccacgttgt tctaagccgg tgcgatcgcc gggcgctcgt ttgtcgcttg ataagtagcg
     2161 gaggtaagaa ggtgaggagt aaaattgttt catcaatcta tgcatcgtaa gaaggagaat
     2221 cagaatgcca aatgttgttg gtttgttcga tagccgatcc gaagcgcacg ccacagtgca
     2281 gcagctggta aattccggtt tcgaccgcga agatatcagc ctcgtttccc aagatactga
     2341 tagacaacca aacgacggta aaactcatcc gggaattagt gggtcggatc tctcgatccc
     2401 gaaggatgat acgagcggag ctctcaaggg cgcgggcatc ggtgccgctc tcggtggaat
     2461 tggtggatta ctggcgggaa ttgctgggct cgctattccg gttatcggac cgatcgtcgc
     2521 ggccggaccg attgcagctg cgctgaccgc cgccttgggc ggcgcgggag tcggcgccgc
     2581 cacaggcggc ttgatcggtg ccttaactga tatgggtgtt cctgaggacg aagccaagca
     2641 ctatcaagat caggtcggac gtggaaaaac actcgtgata gtcagagtaa gcgacgatgc
     2701 ttcggcggat cgtgcggctg atattctgga atcgagcggc gctgaagaga ttgacgggcg
     2761 tgagaccgac actgattaca tccttgatcg tccaagcgca gtcggtggcg agacccagtc
     2821 ccgtgatgtt gaggttagcg aacgccaggc actccgtgat gacaggtctg aagctaatcc
     2881 tggcgagcgc gacatcaact atcgaacggc gcgaatagat gaatcaggag taagagccgg
     2941 tcaagagagc ttcagccctg aggatcgagt aattccagtg actgaagaac agttggttgt
     3001 tggaaaacgg gctgtgcaaa gcggacgcgt acgtatttac ggacgtgtca ctgagaagcc
     3061 ggtcgaacaa tcagtgcaac tacaaaatga acgggtaacg gttgatcgac gtgcggtaga
     3121 ccggcctgtc cgcgccggcg ataaagagga tgacgttgtc atagagatta ctgagacgaa
     3181 agaggagccg gttatcggca agcaagaacg cgtcgttgag gaaatcgtta ttggcaaaac
     3241 gacagaggaa cacaccgaga cggtgcgcga cacagtacgt cgtaaagacg ttgaagtaga
     3301 aagaaccgct catgaggaaa gactgagaga agaagacaat cgacgctaat tcacttcact
     3361 ggattgttgg tcgtcctctc gcatccaaga gggcgaccaa caattcttct ttcccgccgt
     3421 acaggtttcc catagctaag taccgaggag atgtcatggc agccccagta accgccactc
     3481 attgcatctg caggcatcgc gatcatcaag gcagaccgtg tccgaatacc gatttcgatg
     3541 cccaaacatt actctgttca acgtgcaaaa caggccattg aaataagtta catttatttt
     3601 catgcacata cccaaagact tgcttgatgt aagttcgcat tgggtcgcca ttataccggc
     3661 cgccgcatcg agatcggcag cgagccgctc gaaggctcag gtctacctcg agattgagcc
     3721 atcggaagta acctaactga tgatatcgct ggataggttg gatgtgttcg atggtgatga
     3781 gctgactgtc atcatcgaga cgcccaaagg ctcccagaac aagtacgcct acgagccgcg
     3841 gtttggaacc ttcgtgcttg acggcgtact gcccgctggc gccgtcttcc ctttcgactt
     3901 cggctttgtg ccgtcaacga ttggggagga cggcgatccc ctcgacgtgc tggttctcat
     3961 ggatgccgcc gcgtttactg gctgtatcgt tgcgtcacgg ctgatcggcg tcatcgaggc
     4021 cgagcagacg gagaacagca agacctttcg caacgaccgg ctgatcgcag ttgcagtgaa
     4081 atctatcacg catcgatcgc ttcgcgacat cagtgatgtg agcggagact tggtcgggca
     4141 aatcgagcac ttcttcatct cctacaacat ggcaaagggc aagacgttcg agcccaaggg
     4201 gcgctccgga cgggagcggg cgttggccct cctaacgact ggcatggcgc gagccgacag
     4261 agcttgatcg aggcgcgtag gcattcgatc tcaaaaaccg ttcagagaac ctgtgccgca
     4321 cctgattgac gccgctctta gatagttgtg tgtctaaaga cggcggtcga gggattttga
     4381 actccccatc atcgcgtatg cctaatttta ggctaagctt gtcttattag tttttcttca
     4441 aaaaagagat ctatcctggg tttgaagaag ccctttgcgc gtaagacggc agttttgcct
     4501 cccacctcga aagtaatctc tttggggagg tccagccact gagaattatt ccaagtctgc
     4561 gcttcatttc cgtcgaccat tagtttgccg gttcggctgt cattcctgcc atactcaact
     4621 tcaatgaggt gtttcttcgc ttcgatttct actgaccatg tcctgtccat tatgatcctc
     4681 caaaatacgt gagccttccg aatctggtag gccaccgtcc accggttgca ttacgttatt
     4741 cgattcctag acttaatata atccaatggc tggggattgc tgtccaatat aagacaatct
     4801 acgaataatt atcttgttga gcgctgccgg acgaagaaac cgcgagggtt gaactgattt
     4861 ctccttcagt tcaatcgtgc ggggaatgtc ccagcaggta cattggcgat ataatgacca
     4921 ggtatattga attcaaatca aggctgacgg aaattgattc gcaactcgtt gacaggacag
     4981 aggaggagag atggctctgc agcatccaaa catgacagta gcgcatcggc agttgaccgc
     5041 gaatgagcgt cagattctcg agaagcaggt gctcgttcac gttggcatcg ttttgccaga
     5101 cgggacacct cacgtatcgg cgatgtgggt ggatctggac ggtaacgata tcattctcaa
     5161 cacagcggag gggcgaacca aggccaacgt cctgcgcctc gggagccccg tcgcgctcag
     5221 tgtccttgat cctgaaaacc cctaccgcaa cctcggcctg cgcggccgcg tggtcgaggt
     5281 cattagtgaa gagcgggggg ctgatgcagg gatcaaccgg cttgccaaga agtatctcgg
     5341 agtcgaggaa tacccttatc gagccccggg agagcgacgg gtgcagttcc gggtgcggcc
     5401 cgaggtcgtt agcggttggg gcaaagatta agctgagcgg ttttgcgggc aattccgggg
     5461 aacaatatca accgcgtcga tggtcattac agcaacagaa aggaaacgga attgaagcgt
     5521 cgttagttca atgttgcgag gttcggctgt gcactataaa ttcagcgatg tggtggggcg
     5581 cctcacccgg agaaggatca ctcggctccc aatgtctcat agaacttatg ggtggttccg
     5641 cattcaccct gaactcaggt tttggcttag ggtgaacggt atcgcctgga cttcagtccg
     5701 gctggcaatc gcctgcagaa cataa
//
LOCUS       DASUTG010000569         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_682520, whole genome shotgun sequence.
ACCESSION   DASUTG010000569 DASUTG010000000
VERSION     DASUTG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682520"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VF089_13165"
     CDS             <1..595
                     /locus_tag="VF089_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6174961.1"
                     /translation="RISRRRWVGADAAIGVVTTSSFALGVAIISTYRSFTRNIEAALF
                     GNILGITTRDLWIILAISLLVVCALFAMRRQLLFTTFDPEVASAYGVSTDTIDRLFVL
                     LLAVTVLASTQILGVTLVAAAMVTPPVIARYLTRKFHTLLIVSPVLGAFCGAAGMFLS
                     YYFDISSAATIVLTAAALFIVVELIVITRGQLTVAQR"
BASE COUNT          445 a          635 c          543 g          480 t
ORIGIN      
        1 tcgcatcagc cggcgacgtt gggtaggggc cgatgccgct atcggtgtgg taacaacgtc
       61 cagctttgct ctcggggtcg ccattatttc gacttaccgc agcttcaccc ggaatatcga
      121 agcagcgtta ttcgggaaca tcctcggcat aacgacccgc gacctctgga tcatccttgc
      181 gatttcgctg ctggttgtct gcgcgctttt cgccatgcgc cgtcaactgc tgttcacgac
      241 gtttgatcca gaagtcgcct cggcctatgg agtatccacc gataccatcg accggctttt
      301 tgtcttgctg ctggcagtta ccgtcctagc atcaacccaa atactcggtg ttacgctggt
      361 ggccgccgct atggttaccc ccccggtgat cgcccgctat ctcacgcgca agttccacac
      421 actgctcatc gtgtcaccgg tgcttggcgc cttctgcggc gctgcaggta tgttcttaag
      481 ttactatttc gatatttctt cggcagccac gattgtgttg acggcggcgg cgctgttcat
      541 cgtcgtggaa ctcatcgtca tcacgcgtgg ccaattaacc gtggcacaac gctaacatgt
      601 ggagatagat tcggcgtctc ccatgccgga ttacagggaa gaggccgtgc acaagctttc
      661 cccgtcatta cggcagcgta cggcgccatc gaccggcttg agatcggtct cgggattcaa
      721 cgtgtcaata aagatttgcc cggagaagcg cccgccagag gctttgaaga tcttcatctc
      781 cggttaaaaa tcaggatcat gaagaaagtg aaatctggcc tgcggcggcg gtttctgcgg
      841 atgtgagcgt gcggacggca aacaaagcca aaggcctctc caccggcaac tcggatcacg
      901 ctctgtccct ttattctttc acagctctac aaaagggtct ggattgtatc tgacctggaa
      961 tatcttctgt tgacagcctg cgtggtgaaa aacttaacaa tcccctcacc ggccgacgaa
     1021 gccttcagcg tttcgcgcgc gccaaaggga caagaaaggc ggccttccag ctcggggcgc
     1081 gtcgcagagc ctctgcgagc ttcggattga ttgacccatt gctaatacgt taaggttcgg
     1141 cgtgtaagag ccatgcaagc tttaatgccc atagcatcac tggaccgtcg gggaaaaaca
     1201 cgccttgggc tcgccaccgc gtttttgttc ttcgtactct attccacgcc gcatcgggtt
     1261 catcacttct ttgagaatct accttcatca caagctggag atcatgaaac ggcgccagtt
     1321 gccgcttcta gcggaggcca cgaacatgag cacgatcatc acagcggtcc agaaagttct
     1381 gatcctcgcc gaagctccac ctccgactgc gtcgcccaga gcgttgcgca gaattcgcat
     1441 ttatcctttc atcagccgct agaagtcaac tatcacgggc tcgagactaa ggcgcaagcc
     1501 gatcctccgg ttgcagcgcc ctacgagttc ggccgttctc cgtcctccca gcgagcgcct
     1561 ccagcagcct agacttggct atctctcggc agaattccgg gatttttcat cgcggcaaca
     1621 acaactcgca aatcccgccg gcagagcgcc ggaaccgcgg agctacgcgt cttcattagc
     1681 gaggttctat gaacacgaac aacgatttgc cgcctcggat cggttgggca acattccgga
     1741 cacgactact tggtatcgaa ctgtcgctgt gtttattgtt aacagcttgc agcgggcatg
     1801 gcagcacgcc gggggctttc aaccacaccg caaatctcgc ctttggcccg gagcaagaac
     1861 tgggacaaag ccacaacaac ccatcgactc catttctccg ctatagtccg gacggccgat
     1921 tattcgccat ctggaccgaa gctgaccaca gctcgagttc gcagaaaact cactccgcca
     1981 cgcatcaata ccaccagtcc ggcaagatgt cgccgtcgcc tatgcgtaat gcgatcttgg
     2041 cggcgtccat tgacaacggc aaaacttggt cttcggccac acaggtgaat cgctcggtgg
     2101 agg
//
LOCUS       DASUTG010000570         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_237399, whole genome shotgun sequence.
ACCESSION   DASUTG010000570 DASUTG010000000
VERSION     DASUTG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237399"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2807
                     /locus_tag="VF089_13170"
     CDS             <1..>2807
                     /locus_tag="VF089_13170"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6174962.1"
                     /translation="PHAASPQYDVVIFDSVPAPQLAQGNFILINTVAPNLPLRLQGTV
                     KTPRIVYQATKHPITEGLSLGDLHVQSSLRLELNGAGTILARSPESPLIVAIESGKLR
                     ALVIGFDLMDSDLPLRVAFPVLLHNALEWFQPQHVEFPARGAQSGSPISLPVPASVGA
                     IELTKPSGRKETLSSTTNPWIFTDTFEAGLYSYKKSGTESRFAVNLFDESESQIAPRA
                     DLGVAAAEAAQKGDQKLDAGFSLWPSLVIAVVLVLAIELALAFRSRVPLPPLVIRAAA
                     LGALILALLNPRLFQSTTALDVVLDVDISRSVGQEAREKGREVLEVVGRAKKPETRTG
                     MVVFGRAPQWEFLPRENFPAGDTALRLDREETDIQAALQASLAQIGEGRQGKILLISD
                     GNENRGEISRIIPLLRSHGTQVWTLPVSLSRGRNEVYLSDLVSPRQVDSGEGFEIAGK
                     IESLRDAPSRIKLLRDGVLIREQELQLRSGTNQVSFRESLFERGNHSYELLLESREDT
                     LAENNLLQGVVEVKGAPRVLLLSGNHDSQLFLSKVLQVQGYTVVQSAPERTPMTLTEL
                     SSFDLLVLDNVPAFQLTDAKMENIEKYVRDLGGGLLVIGGSQSYGAGGYYRTALERVL
                     PVDMRPPARLDLPHVALLFVLDKSGSMGAGQEGSTKLDLAKAAAVAAADIMNPTDQVG
                     IIGFDAAWDWVLPFRPVGNGEWISEKLAALQSDGGTDLYKALVEAHRAIAAKQAAIKH
                     VIVLSDGLTDKADFHGLGQRMARDGITVSTVSVGSDADVQLMADIAKEGKGRGYVALD
                     PQTIPQIFTAETLLISRDLLVDKLFTPKIVAPVGPLKGISQSGLPPLRGYVLTYPKPR
                     SELLMTADKDPLLVSWRYGVGRVMAFTSDLSGRWGKDFVTWPGFPQWASQLARDTMRK
                     LLETRMRAELQPDGEAVKVVA"
BASE COUNT          554 a          796 c          858 g          599 t
ORIGIN      
        1 atccccatgc ggcgtcacct cagtacgacg tcgtaatctt cgacagcgtg ccggcgcctc
       61 aattggcgca aggaaatttt atcttgatca acacggtggc gccgaatctg ccgttgcggc
      121 tccagggcac ggtcaagact ccgcgtattg tttatcaagc gacaaagcat ccgatcaccg
      181 aaggcttaag tctcggcgat ctgcatgtgc agtcatcgct gcgcttggag ctcaacggtg
      241 caggaactat tcttgcgcgt tcaccggaga gcccgctgat cgtggcgatc gaaagcggca
      301 aactccgagc gctggtgatc ggcttcgatc tgatggattc ggatctaccg ctcagggtgg
      361 catttcccgt cctgcttcat aacgcgctgg agtggttcca gccccagcac gtggaattcc
      421 ctgcgcgcgg cgcgcaatcg ggtagtccca tctcgctacc ggttccggcc agcgtgggcg
      481 ccatcgagct gacgaagccc tcgggcagga aagaaacgct cagttctacg accaatccct
      541 ggatcttcac ggatactttc gaagcgggtc tctatagtta caaaaaatcc ggcacggaaa
      601 gccgcttcgc cgtcaatctt ttcgatgaaa gcgagtcgca gatcgcgccg cgcgccgacc
      661 tcggcgttgc agctgccgaa gctgcccaaa aaggcgatca aaagctcgac gccggttttt
      721 cgctgtggcc atcgctcgtc atcgcagtcg tccttgtgct tgccatcgaa ctggctctgg
      781 catttcgtag ccgggttccg ttgcctccgc tcgtgattcg tgcggcagcg ctgggcgcat
      841 tgatcctggc gctgctcaac ccgaggcttt ttcaatccac cactgctctg gacgtcgtgc
      901 tcgacgtcga tatatcgcgc agtgttggac aggaggccag ggaaaaaggg cgggaggtgc
      961 tcgaagtcgt cgggcgtgcg aagaaaccgg aaacgcgcac cgggatggtc gtgttcggcc
     1021 gcgcgcctca gtgggagttt ctgccgcgcg agaattttcc cgctggggac acagcattgc
     1081 ggttggatcg ggaagagacc gacattcagg cggcgttgca ggcgtcgttg gcgcaaattg
     1141 gcgaggggcg tcaggggaag attcttctta tctctgacgg caacgaaaac cggggggaga
     1201 tctcgcgcat tattcccctc ctgcggtcac atggtactca ggtctggacc cttccggtaa
     1261 gcctgtcgcg cggcaggaat gaagtctacc tgagcgatct tgtctctccg cgccaggtgg
     1321 acagcggcga gggatttgaa atcgccggca agatcgaaag cctccgcgat gcgccgtcgc
     1381 gcatcaaact actgcgcgac ggcgtcttga tccgtgaaca agagcttcaa ttacggtccg
     1441 gaaccaatca ggtgagcttt cgtgaaagtt tgttcgagcg cggcaatcac agttacgaac
     1501 tgctgctcga gtcacgcgag gataccctgg ctgaaaataa tttgctgcaa ggcgtggttg
     1561 aagtgaaggg ggcgccgagg gtgttactcc tctcgggaaa ccacgacagc cagctatttc
     1621 tctcgaaagt gctgcaggtc caagggtata ccgttgtcca atcggcgccg gaaagaacgc
     1681 cgatgactct caccgagcta tcttcgtttg atctcctggt gctcgataat gtgccggctt
     1741 ttcaattgac ggatgccaag atggagaaca tcgaaaagta cgtgcgcgat ttgggcggcg
     1801 gtttgctggt tatcggcgga tctcagagtt atggcgccgg cggctactat cgaaccgccc
     1861 tggagcgggt tctgccggtc gacatgcgcc cgcccgcgcg gcttgatttg cctcacgtgg
     1921 cgctgctctt tgtcctggac aaatccggca gcatgggcgc ggggcaggag ggcagcacca
     1981 agctcgactt agctaaggcc gcagcggtcg cggccgccga catcatgaac ccgaccgacc
     2041 aggtgggcat catcggcttc gatgccgcct gggattgggt gctgccgttt cgtcccgtag
     2101 gcaacggtga atggatttca gaaaagcttg cggcgttgca gtcggatggc ggcaccgacc
     2161 tctacaaagc tttggtggag gcgcatcgcg cgattgccgc caaacaagcc gccatcaagc
     2221 acgtgattgt tctttccgat ggccttaccg acaaggccga cttccatgga cttggccagc
     2281 ggatggctcg cgacggcatt accgtttcga ccgtttccgt aggcagtgat gccgacgttc
     2341 agctcatggc cgatattgcc aaagaaggca aaggcagagg ctatgtcgcg ttggacccgc
     2401 aaacgattcc gcagatcttt accgcggaaa ccttgctgat ctcgcgcgat ctgctggtgg
     2461 acaagctttt cacgcccaag atcgttgcgc cggtaggccc gctgaagggc atcagtcaaa
     2521 gcggattgcc gccgctgcgc ggttacgttc tcacctatcc gaaaccacgc tctgagctgc
     2581 ttatgactgc ggacaaagat ccgctgcttg tgtcgtggcg ctatggcgtc gggcgggtga
     2641 tggcgtttac ctcggattta agcggccgtt ggggcaaaga ttttgtgacc tggcctgggt
     2701 ttccgcaatg ggccagccag ctggcgcgcg ataccatgcg caagcttctt gaaactagaa
     2761 tgcgcgccga attacaaccg gatggcgagg cggtaaaagt cgtcgcc
//
LOCUS       DASUTG010000571         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_533010, whole genome shotgun sequence.
ACCESSION   DASUTG010000571 DASUTG010000000
VERSION     DASUTG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533010"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..934)
                     /locus_tag="VF089_13175"
     CDS             complement(50..934)
                     /locus_tag="VF089_13175"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6174963.1"
                     /translation="MIRGVLLPVITPFDEKVRVDEQMMRQLVDFHIEAGVQGLFVLGS
                     TGQGPAMTIEERKQTAAVALDQAKSRVPVIIHVGTADAGSAVELAEHAAAHKADAVAI
                     VPPYYYSDHTEYEIIAHYKAVHKAVPLPIFIYENPKYSGISIPPGLAVRMKEQVPALQ
                     GIKVAYGQGALLEYVRLLPDISVFTGNADLFGLVPFGVAGMINPPTSFVPELCVDLFK
                     ALDSQDYPRAVEAQKRVDTAARIVAARLRKYGRVPLQEVFRMRGFAVKRFPKWETEQM
                     PKDEVMKLERELKTAGIL"
     gene            complement(1049..2266)
                     /locus_tag="VF089_13180"
     CDS             complement(1049..2266)
                     /locus_tag="VF089_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX6174964.1"
                     /translation="MKKVTLADIPRVGPGTPAGEWFRKYWLVVGTTQDLYDIPQAVKV
                     LGEELVLFRDQTGKLGLLGLHCPHRGTSLEYGDIEDGGIRCPYHGWLFNVAGQCLEMP
                     AEPKDSKFPQKVRHLSYAVREQGGLIFAYMGADQDDPPPLPKYSPLVDRGSQWQIEPV
                     RHADYNWFNFFENSADPAHVCILHRHAGYGQQSWGNQFFSYTDMPHFEFVEMDYGMKV
                     VMTKPGPEPGTEFVDEMSLALPSIVQVGDTEFVHAKIDAAALVKEGSQCEHWMFVTPN
                     DDHHFMLFTADNYRGPEENFFEKLKALRAKEMPAQEVKPYDKRQFMPYKGNIRKEDLV
                     TQGTQGLLGERSEQLGVSDRGVIKFRKIVVDAIERALNGGTPKGIVDKKQAAEVYRLD
                     TQVGVRAKRSNLG"
     gene            complement(2413..3789)
                     /locus_tag="VF089_13185"
     CDS             complement(2413..3789)
                     /locus_tag="VF089_13185"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6174965.1"
                     /translation="MMDLREFLKVLEEEHELQKIKVEVDPKHELGAICKIHNERRDSP
                     ALLFENVKGHTIPVVGQLLASDRRVALALGLSQENVFNETVQRASSPIAPKLISKGAA
                     QDIVFEGADVDITKLPLCTNNPRDGAPYITAGHVIIKDPEYGMNLSIYRMMLVSKNEV
                     TLRFTPGHDGYDFMKNAEKRGQKKFEVAVCIGVPPAVYVASQFEPRIGVYELDIAGGI
                     AGEPVEVVKCRTVDLEVPALAEIVLEGELTIPAKTGDEGPFGEFCGYTTAQVPNERIM
                     TVKAVTHRKNPVYHNIWLGKPPHEHLYVDALTYAVAAYQELKPAYPALKKAYAPPWGV
                     SIVLLLQLEKRLMRPGIVDNILAASLYTRSGKWKHVFVLDEDIVLEDPNEVLWALTTR
                     FQPATDMFIIPRGITSSLEPSATVDGLTSKLMLDLTIKKNFRGEVAEPTAETRESVLK
                     RWKEYGFK"
     gene            complement(3834..>4706)
                     /locus_tag="VF089_13190"
     CDS             complement(3834..>4706)
                     /locus_tag="VF089_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6174966.1"
                     /translation="VTGGSGRLGQYVVRELFTHNHAVASLDALKPRECLCPTYTVDLT
                     KFDSLLEHLNNADALVHLARVRFPYTENGFNVAEQKWEFADIGGDAERFNQNIAITNN
                     VLAAAQACAVKKVVCGSSLAVYGLYYPSTELQPAYLPIDEDHPLRPQDPYGMTKLIGE
                     KLCDALTQKTGAQVASLRLAGVYTEAHRSMLLERKNYPLARGTGALWSYIDARDAARA
                     CRLALEADFGGHHAFNICAPSTIMDMPIRELIARYLPRVTDLRTGLDDYGSGYSIEKA
                     KSMLGFESRCSLVD"
BASE COUNT          986 a         1405 c         1238 g         1077 t
ORIGIN      
        1 gacgattttt ttcataggct caaccagcca tctggtgtcg tcatggcggt tatagaattc
       61 ctgcggtctt cagttccctt tccagcttca tgacctcatc cttgggcatc tgctcggttt
      121 cccacttcgg gaaacgtttt accgcgaagc cgcgcatacg aaaaacttcc tgcaacggaa
      181 cacggccata cttgcgcaag cgcgccgcga cgattcgcgc cgcggtatcg acccgtttct
      241 gcgcttccac cgcgcgcggg tagtcttggc tgtcgagagc cttgaacaag tcaacgcaga
      301 gctcgggcac gaagctcgtc ggcggattga tcataccggc aacaccgaac ggaaccagtc
      361 cgaataagtc cgcgtttccg gtaaaaaccg agatatcagg cagtagtctc acgtattcga
      421 gcaacgcgcc ctgaccatag gcgactttga tgccttggag cgccggcacc tgctctttca
      481 tgcgcacggc caaccccggc ggaatagaaa taccggagta cttgggattc tcgtagatga
      541 agatcggcag cggcacagct ttgtgtacgg ctttgtagtg cgcgatgatc tcgtattcgg
      601 tgtgatcgga atagtagtat ggcggcacga tagccaccgc gtccgccttg tgcgcggcgg
      661 catgctcggc caactccacg gcactgcccg catcggcggt gccaacgtga atgatcaccg
      721 gcacccggga cttcgcctga tcgagcgcca cggcggccgt ctgcttgcgc tcttcgatgg
      781 tcatggcggg gccctggccg gtggagccaa gcacgaacag tccctgcacg ccggcttcga
      841 tgtgaaaatc gaccaactgc cgcatcatct gttcatcgac ccgaactttt tcgtcgaacg
      901 gcgtgataac cggcaacaaa actccacgaa tcattcattt ctccttgtaa aaatcagttc
      961 ccacatttgg agtgagcacg cgcagaatgt ctcccaggag cgccgcgacg aagcatctgc
     1021 tttgaatctc accaacgagc gtgactccct aacctaagtt ggatctcttc gcccgcactc
     1081 ccacctgcgt atccaaccga tacacctcag ccgcttgttt tttatcgaca atgcccttgg
     1141 gcgtgccgcc gtttagcgcc ctctcgatcg catccaccac gatcttgcga aacttgatca
     1201 cacctcgatc ggacacgccg agctgttcgc tccgctcgcc gagcaaaccc tgcgtcccct
     1261 gggtgaccag atcctcctta cggatattgc ccttatacgg catgaactgg cgcttgtcgt
     1321 agggctttac ttcttgcgcc ggcatctcct tcgcgcgtag ggctttgagt ttttcgaaga
     1381 aattctcttc cggtccccgg taattgtcgg cggtaaaaag catgaagtgg tggtcgtcgt
     1441 taggggtgac aaacatccag tgctcgcatt gcgaaccttc cttgaccagc gccgcggcat
     1501 cgatcttggc atggacgaac tcggtatcgc ccacctggac gatactcggt aacgccagac
     1561 tcatttcatc gacgaattcc gtgcccggct ccgggccggg cttggtcatg acgactttca
     1621 tgccgtagtc catctcgaca aattcaaagt gcggcatgtc cgtataactg aaaaactgat
     1681 tgccccacga ttgttggccg tagccggcat ggcggtggag aatacagacg tgggcggggt
     1741 ccgcggagtt ttcgaaaaaa ttgaaccagt tgtagtcggc gtgacgcact ggctcgatct
     1801 gccactggct gccgcggtcg accagcgggg aatattttgg cagcggcggc ggatcatcct
     1861 gatctgcgcc catgtaggcg aagatcaagc cgccttgctc gcgcacggcg tacgacaagt
     1921 gcctcacctt ctgcggaaac ttgctgtcct taggttccgc cggcatttcc aagcactggc
     1981 cagcgacgtt aaacaaccag ccatgatacg ggcagcgaat cccgccatct tcaatatcgc
     2041 cgtattccaa cgacgttcca cgatgcgggc agtgtaagcc cagcaggccg agtttgccgg
     2101 tttggtcgcg aaacagcacc agctcctcgc caagaacttt taccgcttgc ggaatgtcgt
     2161 acaggtcctg agtcgttcca acaaccaacc agtatttgcg aaaccactca cccgccggcg
     2221 tccccggacc tacccgcgga atgtctgcca gcgttacctt cttcatcttt ctcttggcag
     2281 ctgcttttac cactctgcac ccctattgat aatctgacgt tgaaccttga accgttgaac
     2341 ttttgaacga ttgaaccgct tttagcgatt ggaaccgttg gaacctatgg aacagctgga
     2401 acttttcagg tcctacttga atccgtattc cttccatctc tttaatacgc tttcacgcgt
     2461 ttcggcggtc ggttcagcga cctcgccgcg gaaattcttt ttaattgtaa gatcgagcat
     2521 cagctttgac gtcagtccat cgacggtggc cgatggctcg agcgagctgg tgataccgcg
     2581 cgggatgatg aacatgtccg tggccggctg aaaacgagtg gtcaacgccc agagcacttc
     2641 gttgggatct tccagaacga tgtcttcgtc gagaacaaac acatgtttcc atttcccgct
     2701 tcgggtgtag agcgacgcgg caaggatgtt atcgacgatg cccggtctca tgagccgctt
     2761 ctcaagctgc aacagcaaaa caatcgaaac cccccacggg ggtgcgtagg ctttcttcaa
     2821 tgccggatag gccggcttca actcttgata agccgctacc gcataggtca atgcgtcgac
     2881 gtagagatgc tcgtgcggcg gcttgcccag ccagatgttg tgatagaccg gattcttgcg
     2941 gtgggtcaca gctttgaccg tcatgatgcg ttcgttcggc acttgcgccg tcgtatagcc
     3001 gcaaaactca ccgaacggcc cctcgtcgcc ggttttggcc ggaatcgtca gttccccttc
     3061 gagaacgatt tccgcaagcg ccggcacttc gagatcgacg gtccggcact tcaccacctc
     3121 gacgggttcg ccggcaatcc caccggcgat gtctaattca tagacgccga tcctcggttc
     3181 gaactgcgat gccacataga ccgccggcgg cacgccgatg caaaccgcga cctcgaattt
     3241 tttttggccg cgtttctcgg cgttcttcat aaagtcatag ccgtcatggc ccggcgtaaa
     3301 acgaagggtg acttcgttct tcgacaccag catcatccga tagatcgaaa gattcatgcc
     3361 atactcgggg tctttgatga tgacatgacc ggcagtgata tagggcgcgc cgtcgcgcgg
     3421 attgttggtg caaagcggca atttggtgat atcgacgtcg gcgccttcaa agactatgtc
     3481 ctgggcggcg cctttggaga tcaacttcgg cgcaatcgga ctgctcgccc gctgcacggt
     3541 ttcgttgaac acattctctt gggataaccc cagcgccaag gccacccgcc ggtcgctcgc
     3601 cagcaattgt ccgaccaccg gaatcgtatg acctttaacg ttttcaaaca gcagagccgg
     3661 actgtcccgc cgctcgttgt ggatcttaca gatcgctcca agctcgtgct tcggatcgac
     3721 ttcgactttg attttctgca gttcgtgctc ttcttccagg accttcaaaa actctcttaa
     3781 gtccatcatg tcaaaatggc tcccgtcatt tcccagtaag tgaaaaactt tgcttaatcg
     3841 actaatgagc agcgcgactc aaagcccaac atgctcttcg ctttctctat actgtaaccg
     3901 ctgccgtaat catccagtcc cgtccgcaga tcggttaccc gcggcaaata acgcgcgatt
     3961 aactctcgga tgggcatgtc catgatggtc gaaggggcac agatgttgaa agcgtgatgg
     4021 ccgccgaaat cagcctccag ggccagccgg caggcgcgcg ctgcgtcccg cgcatcgata
     4081 tagctccaga gcgcaccggt gcctcgcgca agaggatagt tcttgcgctc gagcagcatc
     4141 gaccgatgcg cctccgtata gacgccggcc aagcgaaggc ttgccacctg cgcgccggtc
     4201 ttttgagtca aggcatcgca gagtttttcg ccgatgagct tggtcatacc gtaaggatct
     4261 tgcggccgca aaggatggtc ttcgtcgatc ggcagatagg caggctgcag ttcagtggaa
     4321 ggataataga gaccatacac ggctaggctc gatccgcaga cgactttctt gacagcgcac
     4381 gcctgcgccg cggccaggac gttgtttgtg atcgcgatat tttgattgaa acgttcggca
     4441 tcgcctccga tatcagcaaa ctcccacttc tgctcggcca cgttgaagcc attctccgta
     4501 taggggaaac gcaccctggc aaggtgcacc agcgcatcgg cgttgttcaa atgctccagc
     4561 aatgagtcga actttgtcag atcgacggtg taggttggac acaaacattc gcgcggcttc
     4621 agcgcatcca gagaggccac cgcatggtta tgggtaaaca gttctcgaac aacatactgc
     4681 cccaaccgtc cggagcctcc ggtgac
//
LOCUS       DASUTG010000572         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_533021, whole genome shotgun sequence.
ACCESSION   DASUTG010000572 DASUTG010000000
VERSION     DASUTG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533021"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1529)
                     /locus_tag="VF089_13195"
     CDS             complement(309..1529)
                     /locus_tag="VF089_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6174967.1"
                     /translation="MNLPYREHFGLKQEPFNITADPAFLYLSRSHREALAQLSYGIKA
                     RKGFVVLTGEVGTGKTTLINGLLDELDSDSRTALIFSHIANPFDLLRYTCNEFGLRSP
                     QHSRDDFYEYLALLNDYLFESYRSGKNCALIIDEAQNLSSEVLESVRLLSNFETSKDK
                     LLQILLVGQPELSTRLNSQELRQLKQRVTVRYHLRALTLKECQAYISSRLQVAGGDPM
                     LFTSKAVEVIYACSAGIPRVINVICDGALMTAYSLRKSYIDDSLIRQVAEDLQIGGTV
                     FSGENLSKSTNEMISPTANEVASQPQEVSKPSSLQPPVVKVEESAGTRSGVVPRIVFD
                     ELSVILIDAMGPMASIVLKDRAASLGESLDRFPVDKLKALIELVSDEILDPSMRQDFQ
                     QHVQERVMPRVDLD"
     gene            complement(1804..>3066)
                     /locus_tag="VF089_13200"
     CDS             complement(1804..>3066)
                     /locus_tag="VF089_13200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6174968.1"
                     /translation="TLYTDESMAGTGARERHDATRPKAHILIIDDELLPREQLVGFYT
                     SNGYTVDIADSCEEGLNRVKQGKIDLVIFYSDVTDCSGGEVLQQLERDHPGVSVILTG
                     YWPIDYHTNALQYGTCDYMPKPFDYETIQSKTQVALNKACVFAESTSLRQLLNDTLGE
                     FGLLLSKTPAMQQIFETMRMASPTNMSVVLEGEAGTGKELIASAIHYQSSRRNGPFIA
                     VNCAAHPDFQLETELFGCEDGARFKNELKRPGKIKLADGGTLFLDEIESMAPIVQSKF
                     LQVLEDCNSVHQGGQQSLDSDVRVIAASNVPLQELIANGKMRVDLYYRINVVPIHVLP
                     LRERSGDIPLLVDDFLRWHPIANQKRIVGVSRETMARLIQYSWPGNIRELRNVLEKGI
                     VFTKGSVIEKIDLPVGKEPVNRDASTFA"
BASE COUNT          776 a          780 c          742 g          768 t
ORIGIN      
        1 aattaccgga cattatcgcg gacacccgtt ggagcagtgt ccaagcgaaa tcaaatggtg
       61 cggaatgtgg caggctgatt cgagaggatg agagaatcta agctgtcatg gcagagtaga
      121 taatactagc aggtgctgaa aaaagcctct tgcaggttgc tcaaaaagat ccagaggcaa
      181 gacgcgcgaa aatcgacgag cggaggcgta cttacagtac gtcgcagcga ggcaattgag
      241 cggcaacgat gcatatgggt ctttttcagc aacctgctag acgaagctga ggcgtgaggt
      301 gcttatcgct aatctaaatc gactcgcggc ataacgcgtt cctggacatg ttgttgaaaa
      361 tcttgccgca ttgaaggatc cagtatttca tcactgacaa gttcgatcag cgcttttagc
      421 ttgtctactg gaaatcgatc gagagattcc ccgagactag ccgcgcgatc ttttaatacg
      481 atcgatgcca tcggccccat tgcatcgatg agtatcacac tcaattcatc gaagactatc
      541 cgaggcacaa caccgcttcg tgttcctgca gattcctcta ctttcacgac gggcggttgt
      601 agagaactcg gcttagaaac ctcctgaggc tgtgaagcca cctcattcgc cgtcggcgaa
      661 atcatctcat tcgtgctctt gctcagattc tcgcctgaaa agacggtgcc tcctatctgg
      721 agatcttctg cgacttgccg gattaaggaa tcatcgatgt agctcttccg taaactgtac
      781 gcggtcatca gcgcgccatc gcagattacg ttgatcactc tcggaattcc cgctgaacag
      841 gcatatatga cctccaccgc ctttgatgtg aatagcatcg ggtcgccacc cgcaacttgc
      901 aatctactcg agatatatgc ttggcactct ttcaaagtca gggcgcgcag atgatagcgg
      961 acagttaccc tctgtttgag ctgccgcaat tcctgtgagt tgaggcgtgt agataattcc
     1021 ggctgcccga caaggagaat ctgcaatagc ttgtccttgg aagtctcgaa gtttgacaac
     1081 agccgcacgc tttccaaaac ctctgatgag agattttgtg cctcatcgat gatcaacgcg
     1141 cagtttttgc cggagcggta actttcaaac aagtagtcat taagcagagc aagatactcg
     1201 tagaaatcat cccgtgagtg ctgcggcgac ctaagaccaa attcgttgca ggtatagcgg
     1261 aggaggtcaa atggattggc gatgtgactg aagatgagag ccgttcggga atcgctatcg
     1321 agttcgtcca ataggccgtt gataagagtc gttttaccgg tgccaacctc gcctgttaac
     1381 acgacaaagc ctttgcgggc tttgatgccg tatgacagct gcgccagagc ctcacgatgg
     1441 cttcggctca gatacagaaa cgccggatcg gcggtgatat taaaaggttc ttgtttaagt
     1501 ccgaaatgct ctctgtaagg caaattcatg aaaactcaat tcagacgatt tatatgtcga
     1561 tccttcatca gatggcaggc tagcagatat caacattgta ggaaatgatt gcttttctag
     1621 ctacagtttc gaaaccggaa gaagtcgcgc cgcctgccat tttctcgacg caaatcaacc
     1681 tacctgccaa tttaacttat agactgcggc atcggacctt taaacgtaac cgcgcgcaag
     1741 ctattcgtgg agccaaatgc ttgcgcgttg gcggtgttga ataagcagcc cctaccgccg
     1801 aactcacgcg aacgtacttg catcccggtt aaccggttct ttcccgacgg gtaaatcgat
     1861 tttctcaatg acagaccctt tagtgaatac aatccccttt tccaatacat ttcgcagttc
     1921 acgaatattc ccaggccaag agtattgaat gaggcgggcc atcgtctcac gggatacacc
     1981 aacaatacgc ttttgattgg caatcgggtg ccaacgcagg aagtcgtcga ccagcagggg
     2041 aatatcgccg gaccgctcgc gcaaaggtaa aacatgaata ggaacaacat tgatccgata
     2101 atacaggtca actcgcattt ttccgtttgc aatcagctcc tgtagaggga cgttagaagc
     2161 ggcaatgacg cgcacgtcac tatcaagact ttgctgtccg ccttgatgca cactattgca
     2221 gtcctcgagc acttgcagga acttcgattg cacgatggga gccatgcttt cgatctcgtc
     2281 cagaaacaaa gtccctccat cggctagttt aattttccct ggacgcttga gttcgttctt
     2341 gaaacgggct ccgtcttcgc agccaaaaag ctcggtttct aactggaaat caggatgcgc
     2401 ggcgcagtta acggcaatga aaggtccatt tcgtcgagaa ctttgataat gaatcgcact
     2461 ggcaatcagc tctttcccgg ttccagcttc gccctcgagg accacgctca tattcgtcgg
     2521 cgatgccatt cgcatagttt caaaaatttg ctgcatcgca ggcgttttgc tgagcaaaag
     2581 cccaaattcg cctagagtat cgtttagcaa ctgacgaaga cttgtacttt cggcaaaaac
     2641 gcatgcttta ttcaaagcca cctgcgtttt gctctgaatc gtctcatagt caaacggctt
     2701 aggcatgtag tcgcaggttc catattgcaa cgcattggtg tggtaatcga tgggccaata
     2761 accggtcagt atgacggaca ctccaggatg atctcgttct aactgctgga ggacttctcc
     2821 tccgctgcaa tccgtgacat cactgtagaa gattacaaga tcgatcttcc cttgcttgac
     2881 ccgattcagt ccttcctcgc acgaatcggc tatatcaacc gtataaccat ttgacgtata
     2941 aaaaccgacc agctgctccc tcggaagaag ttcgtcatcg atgattagga tgtgggcctt
     3001 gggtcgagtg gcatcatgcc tttctcgcgc gccggtaccc gccatagact catctgtgta
     3061 gagtgt
//
LOCUS       DASUTG010000573         2679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_89620, whole genome shotgun sequence.
ACCESSION   DASUTG010000573 DASUTG010000000
VERSION     DASUTG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89620"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF089_13205"
     CDS             complement(<1..312)
                     /locus_tag="VF089_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174969.1"
                     /translation="MTRLVALMLLFLFAVSTAVYSAARAAEPIRIAYSSVNPHALLVW
                     IAEKRGLYAKNGLSSTLVYVPGGSVAIQALVSGDIDLAQLTGPPGVIANMKGADVIYI
                     AM"
     gene            complement(331..1374)
                     /locus_tag="VF089_13210"
     CDS             complement(331..1374)
                     /locus_tag="VF089_13210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174970.1"
                     /translation="MAHFPVIDTDGHVLERQSDIRKYLEPPWDKRPTPLWPGDQPWDN
                     DMMDSFEMARQWKGLSAAEQVERWHAIMDEHGIEKAICFPTGSGNIAKNQEVPYQIAV
                     ARASNTHFSKEYNDRSDRLFCVGCLPMRAPSAAAAELSRGVNELGLKGFEVLPTGLPL
                     ALGDTFYDPIYAEAEKFGAVLGIHGTRNTSRELGTSGLATFSEVHSYAFPVGILLQFT
                     SIVCQGLTIRFPNLKLAFLEIGATWLPYYLDRLDEHWEKRGEYEMPLLKTKPSDVVRR
                     SKMYFSVEPGESQLAATVDYVGAEHFLYASDIPHWDNEFPENLIDLRDHQDLSDHAKK
                     KILYENAKELFAL"
     gene            1762..2232
                     /locus_tag="VF089_13215"
     CDS             1762..2232
                     /locus_tag="VF089_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174971.1"
                     /translation="MALHVRSSDVTEIKRVQSGEAGTGSMVVRKGYGNECSLMIATRA
                     PGYHTKPHQHESEQINYVLDGEIWFFVEDKGFHCKKGDFQRIPANTVHWAWNRSDAEA
                     TVAEAHAPALIGGRAGEYAVGLFDDGETPQVRGPGINQFVPFDQEKAEAKYAGK"
     gene            2243..>2679
                     /locus_tag="VF089_13220"
     CDS             2243..>2679
                     /locus_tag="VF089_13220"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174972.1"
                     /translation="MPWKVSLIVSFLCGLLSLEERSWAQDLRRIHYGTTNSTSHLPVW
                     VAKDAGYFAKSGLNVEPVHIRGGALITMGIMSGQLQFSGAGAESVVAARIEGGDVVLL
                     ACPVYSDPVYLMARPEIKSAAELKGKSTAVTRLGSTTHFYLRAA"
BASE COUNT          612 a          746 c          746 g          575 t
ORIGIN      
        1 catggcgatg tagatgacgt ccgcgccctt catattggcg atgaccccgg gaggcccggt
       61 caactgagcc aagtcgatgt ctcccgaaac cagcgcctga atcgctaccg agccgccggg
      121 aacgtagaca agcgtcgatg agaggccatt tttagcatac agccctcgtt tttccgcaat
      181 ccagaccagt aaagcgtgcg gattcaccga gctgtaggcg atccggatcg gctcggcagc
      241 ccgggcggcg ctgtacaccg ccgtggatac cgcgaacaga aatagcagca tcagggcaac
      301 aagtcgggtc atgagtacct ccgctgatct ttacagagcg aacagctcct tggcgttctc
      361 ataaagaatc ttcttcttgg catggtcgga caagtcttga tgatcgcgta aatcgatcaa
      421 gttttctggg aactcattgt cccaatgcgg aatgtccgaa gcgtagagaa aatgctccgc
      481 gccgacataa tcaaccgtgg cggcgagctg cgattcaccg ggctcgacac tgaaatacat
      541 cttcgatcgc cggacaacat cgctgggctt ggtcttgagc agcggcattt cgtattcacc
      601 gcgcttttcc cagtgttcgt ccagccgatc caagtaatat ggcaaccaag tcgccccgat
      661 ctccagaaag gcgagcttca ggttcggaaa acggatcgtc aggccctgac aaacgatgct
      721 ggtaaattgc aggagtatgc cgacgggaaa ggcgtaggag tgaacttccg aaaaagtagc
      781 caacccggat gtgccgagct cacgcgacgt gttgcgggtg ccgtgaatgc cgaggaccgc
      841 gccgaacttt tcagcctcgg catagatcgg atcgtagaaa gtgtctccga gtgccagcgg
      901 caaaccggtc ggcaacacct caaagccttt caaccccagc tcattaaccc cacggctcaa
      961 ctctgcggcg gctgctgacg gcgcgcgcat cggcaaacag ccgacgcaaa acagccgatc
     1021 cgagcggtcg ttgtactctt tcgaaaagtg cgtgttgctc gctcgcgcca cggcaatctg
     1081 gtacggcact tcttggtttt tggcgatgtt gccggagcca gtaggaaagc agatcgcttt
     1141 ttctataccg tgctcgtcca tgatcgcgtg ccaacgctca acttgctcag cggcgctgag
     1201 tcctttccat tgccgggcca tctcaaagga gtccatcatg tcattgtccc agggctggtc
     1261 gccgggccat aacggcgttg gacgtttatc ccaaggcggc tcgagatact tcctgatgtc
     1321 ggactgccgt tctaagacat ggccgtcggt atcgatcacc ggaaaatgcg ccataaaacc
     1381 tcctaagtta acctacagta agaaaacctt tagtccggtt caaagggatg ttcaatggct
     1441 aacttcggga gaataagtta atgcaccacc ggcattccat gtaatagtcc tcggcgatag
     1501 tttgccaagt cattgttcct caactacgct gtcgagggct gatcaaacac atcacgagtt
     1561 actgtttaca tcatgcaatg cagccttgcg ggagtcgatg atggtattgt caatcttgtc
     1621 tctgatagtc gagttctgat cttctttcgg ggttgagaca atccaaagat caactctcga
     1681 aacgccatgc acatcgcttg acgagattgc ccgcatggca tacgacaaaa gagaactgct
     1741 cacttcccca ggaggcgtca catggctcta cacgtcagaa gctcggacgt gaccgaaata
     1801 aagcgcgtcc aaagcggcga ggccggtacc ggatcgatgg ttgtccgcaa agggtatggg
     1861 aacgagtgca gcctgatgat cgcgacgcgc gcgcccggct atcataccaa accgcaccag
     1921 catgaatccg agcagataaa ctatgtgctc gacggcgaga tctggttctt tgtagaggac
     1981 aagggctttc attgtaaaaa aggcgatttc cagcgtattc ccgccaacac ggttcattgg
     2041 gcttggaatc gctcagatgc cgaagcgacc gtggctgaag cgcacgcgcc ggcgttgatc
     2101 ggtggacgcg ccggtgagta tgccgtgggg ctttttgatg atggagagac gccgcaggtc
     2161 cgcggtccgg gaattaacca gttcgtgcct ttcgaccagg agaaagctga agctaaatac
     2221 gccggcaagt aattgaagcg ccatgccttg gaaagtttca ttgatcgtga gctttctttg
     2281 tggactgctg tcgctcgagg agcgaagctg ggctcaggat ctccgccgca tccactacgg
     2341 cactaccaat tccacctctc acctgccggt ctgggttgct aaagacgcag gttacttcgc
     2401 caaaagcggc ctgaacgtcg aacccgtgca catacgcggc ggagcgttga tcacgatggg
     2461 aatcatgagc ggccagctcc agttctccgg ggctggcgct gagtcggtag tcgcagcccg
     2521 catcgagggc ggcgatgtgg tcttgcttgc ctgcccagtc tactccgatc cggtgtattt
     2581 gatggcccgg cctgagataa agtcggcggc cgagttgaaa ggcaaatcga ccgccgtcac
     2641 ccgactcggc tcgacaacgc atttctatct cagggccgc
//
LOCUS       DASUTG010000574         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1719443, whole genome shotgun sequence.
ACCESSION   DASUTG010000574 DASUTG010000000
VERSION     DASUTG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719443"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="tcuA"
                     /locus_tag="VF089_13225"
     CDS             complement(<1..1422)
                     /gene="tcuA"
                     /locus_tag="VF089_13225"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6174973.1"
                     /translation="MTTKHASVVVVGGGSAAFEAAVAAKQAGAPSVIMLEKAPEAEFG
                     GNARFSHTGFRFVYSGADEIRRFLPFMDEASFSKLQLPGYRPEHFTADLMRVTRGRIN
                     KDLAEVLVNESNGAIHWMRELGIQWEPAGSVEIDGKRYFEPGIVIHPTGGVGGGVSQL
                     NQWRAIASRMGIDIYFDSRVRELLGNDRHIEGVRISNTNEEYDLRAGAVILCAGGFQA
                     NPEMRARYLGANADLMKVRGSRHDTGEVLQMALGLGCKASGHWQGAHATPIDATFPDV
                     ELSNKANRYGYTFGITVNSLGQRFFDEGEAHHSYTYAKTGWAVLGEPGGVAYQIYDRK
                     GAALCDKSYYEFATPVEAPTVAELASRIGIDAAILQHTVDQFNRAVRDEIPFDATRAD
                     GRCTEGITPRKSNWALRIDEPPFQAYPVTGGITFTFGGLEVNRQAQVLNTGGKPIRGL
                     FASGDILGLFFHNYPSCTGQTRNV"
     gene            complement(1459..2466)
                     /locus_tag="VF089_13230"
     CDS             complement(1459..2466)
                     /locus_tag="VF089_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174974.1"
                     /translation="MALYHRKILIIFALAAGFLPAQSPAQIKSNFSSSITSESMAAVW
                     IAKDRGLFKKYGLDVQYILMPRSPLAVSALIAGEIDMAVIGPGHLVNAATGGTDVIGI
                     ANFAQKLDYRLNTRPEIKKKEDLRGKRIAISGPGSTSHLVSLLALQHLGLDPNAAKIA
                     FLTISGTEINRRLALESGSIDATTLNGSIGDLYGQKGYPVLFNFKGSGVTMPQTMLVT
                     TRRLASTKPQVVEAYAKTIVEAIAVMFDPANKETVTRTIASNLRLSNPSDAEEAYQSV
                     INSYERVPYPSLEGMHRLHGLLVTINPKLAGVKIDTVVDDSFIRKLDSSGFVQSVSKK
                     R"
     gene            complement(2500..2892)
                     /locus_tag="VF089_13235"
     CDS             complement(2500..2892)
                     /locus_tag="VF089_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6174975.1"
                     /translation="MPKLRHLAIRTEDTGKLATFYMDVFEMQVLHKDKNEGGAIYLTD
                     GYFNLAILPNHEQQSPNGLYHFGFEVENGEKIVERLKKFNPNKLPKQRPNGRPYAETR
                     TSDIDGNYFDISEHGFLTVEPVGEKKPN"
BASE COUNT          688 a          867 c          836 g          724 t
ORIGIN      
        1 cacgttccgt gtctgacctg tgcaggatgg ataattgtgg aaaaacagtc cgagaatatc
       61 tccggaagca aatagcccgc gaataggctt gcccccggtg ttaagcactt gcgcctgccg
      121 atttacttca aggccgccga acgtaaaggt aatcccaccg gtcaccggat aagcctggaa
      181 aggcggctcg tcgatgcgca gagcccaatt ggatttgcgc ggtgtaattc cttcggtgca
      241 gcggccgtcc gcgcgggtag catcgaatgg gatctcgtcg cgcactgcac gattgaactg
      301 atcaaccgtg tgctgcagga tcgcggcatc gatgccgatc ctcgaagcga gctccgcgac
      361 cgtcggagct tcaaccggag tggcaaactc gtagtagctc ttatcgcaca gcgcggcgcc
      421 tttgcgatcg taaatttgat aggcgactcc gcccggctcg cctaaaaccg cccacccggt
      481 tttcgcatag gtataggagt gatgcgcctc gccttcatcg aagaagcgct gtcccagcga
      541 gttcacggtg atgccgaagg tatagccgta acgattggct ttattgctaa gttctacatc
      601 gggaaatgtc gcatcgatcg gtgttgcatg cgccccctgc cagtgaccgg aagccttgca
      661 gcctaaacca agcgccatct gcaacacctc gcccgtgtca tggcggctgc cgcgcacctt
      721 catcagatct gcgttggcgc ccaaatatcg cgcgcgcatt tccgggttgg cttgaaaccc
      781 gccggcgcag agaatgaccg ctccggcgcg cagatcgtat tcctcgttgg tattcgatat
      841 gcggacgcct tcgatatggc ggtcattgcc gagcagttca cgtacccgag agtcaaagta
      901 aatatcgatc cccatgcggc tggcgatagc gcgccattga ttgagttgac tgacgccgcc
      961 gccaactccg ccggtgggat gaatcacaat gccgggttcg aagtaacgtt tgccgtcaat
     1021 ctccaccgag ccggccggct cccactgaat tccaagctcg cgcatccaat ggatggctcc
     1081 gttagactcg tttaccagca cttcggctaa atctttgttg atgcggccgc gggtcactcg
     1141 catcaaatcg gcggtgaaat gctcgggccg gtagccgggc aactgtagct tgctaaaact
     1201 ggcctcatcc atgaagggca aaaaccggcg gatctcatcc gcccctgaat agacaaagcg
     1261 aaagccggta tgagagaagc gagcattgcc accgaattcc gcctccggcg ccttttccaa
     1321 catgatcacc gacggcgcgc cggcttgttt ggcggcaact gccgcctcga aagctgcgct
     1381 gccaccgccg acgacaacta ccgaagcatg cttagttgtc ataagaacat tggcgacagt
     1441 tggtactgat aatttcagtt aacgcttctt cgagacgctt tgaacaaagc ctgagctgtc
     1501 gagttttctg atgaacgagt catcgaccac cgtgtcgatc ttgacgccgg caagtttcgg
     1561 attgatggtg acgagcaatc cgtgcaggcg gtgcattcct tccaacgacg gatagggcac
     1621 ccgttcataa gaattgatta ccgactggta agcttcctcc gcatcactcg gattcgatag
     1681 tcgcaagttc gaagcgatgg tgcgtgtgac ggtttctttg ttggctggat cgaacatcac
     1741 cgcgatcgct tcgacgatgg tcttcgcgta cgcttccaca acctggggtt tggtcgatgc
     1801 caaccggcgt gtggtgacga gcatggtttg cggcatcgtc actcccgagc ccttaaaatt
     1861 gaacagcacc gggtagccct tctgcccata gagatcgccg attgagccgt ttagtgtcgt
     1921 ggcatcgatg ctaccgcttt caagggcgag tcgacgattg atctccgtcc ccgaaatcgt
     1981 cagaaaagcg atcttggctg cattgggatc gaggcctaag tgctgcaatg ccagtaacga
     2041 aaccaaatga gatgtagagc cggggccgct gatggcgatc cgtttgccgc gaagatcttc
     2101 cttctttttg atctccggtc tcgtgtttag acgataatcg agtttctgcg cgaaattggc
     2161 tatgccgatc acatcggtgc cgcccgtcgc cgcattcacg agatgtcccg ggccgatcac
     2221 cgccatgtcg atctcgcctg cgatcagcgc ggaaacggcc agcggagaac gcggcattaa
     2281 aatatattga acatccaatc cgtacttctt gaacaggccg cggtctttag cgatccacac
     2341 cgcggccatg ctttcgctgg taatgctgga agagaagttc gatttgatct gtgcaggaga
     2401 ttgggccggt aaaaaaccgg ctgccaacgc aaagataatg agaattttcc gatggtagag
     2461 ggccatcgaa acgctgcctc cttcttcctc gaacaggtct tagttcggct tcttttcgcc
     2521 tacaggttca accgtcagaa acccgtgctc ggagatatcg aagtagttgc cgtcaatgtc
     2581 agacgtgcgg gtttcggcat aaggtcgccc gtttggacgc tgcttaggca gcttattggg
     2641 attgaatttt ttcaacctct cgacaatctt ctctccattt tcgacttcga agccgaagtg
     2701 atataagccg ttgggcgatt gttgctcgtg attgggcagg atcgccaaat tgaaataccc
     2761 atccgtgaga tagatggcgc ctccctcatt cttatccttg tgcagcacct gcatttcgaa
     2821 aacatccatg tagaacgtgg cgagctttcc agtatcctca gttcgaatcg ctaaatggcg
     2881 aagcttaggc ataactttcc tccttcgtga ctttgactca accctttatg cccggccggt
     2941 gatcttgtca agtaaaggta aatgcctgcg gcgcgtgaaa gttgaagcgg tcccggctcg
     3001 ttgtagattt actcggagat aacgatcgga aaatctgatc ctttggaact cccaacagga
     3061 cggaaacacg ccacccatcc agacactgcg gcagaatcca acttctggat aaagc
//
LOCUS       DASUTG010000575         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1870080, whole genome shotgun sequence.
ACCESSION   DASUTG010000575 DASUTG010000000
VERSION     DASUTG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870080"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF089_13240"
     CDS             <1..257
                     /locus_tag="VF089_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174976.1"
                     /translation="WPVQNGVIAWNKVVDISDVLTGKAKGRSDDDQIILYKNQGGQGI
                     VDIALAKKCYDLAKQHGKGHELRIEARENWWVQGGRTQTW"
     gene            277..1371
                     /locus_tag="VF089_13245"
     CDS             277..1371
                     /locus_tag="VF089_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174977.1"
                     /translation="MLSKSGRISIPKTIFQKVKALLMIAVAAAFLLGTGTITASHAQA
                     PYYQGKTITVVVGTVAGDLYDLYARAFAQHMPKHIPGNPNMIVQNMPGAGHMIAANYL
                     YSVAKPDGLTIGATLPTLYFEQLIGRAEVKFDFSKFTWLGNATKSPQVLYMRADAPYK
                     SIEDIRNAKEAPKCGTTGTSNMGYFVPKLLEETVGAKFNVIAGYPGGSEVDLAVEKGE
                     VQCRSLSSEAFFSREPFFTWRKNNFVRVLMHGAKEPDERLPGVPSIYELMDKYKTAES
                     GRRLATALMAAGEFHRPYLAPPNLRPEHVKILREAFLKTAKDPAFLEETKKKKLDLDP
                     SSGEEVATLVKEVMSQPADVVERLKKLMGR"
     gene            complement(1483..1914)
                     /locus_tag="VF089_13250"
     CDS             complement(1483..1914)
                     /locus_tag="VF089_13250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6174978.1"
                     /translation="MAAEFIDLLNLPELDIGHSKKVLFTSDHFHTWVHGDYPGTKGPM
                     HKHTADQQFYCVKGECTFHFPDSPARKLTPGMLVTIPAGQLYQLDNTGDEYMILLGAR
                     AEAAAKPRFSDKDEVVTTGDYAAQNLDKIKAPEEKKAKAKL"
     gene            complement(2104..>5354)
                     /locus_tag="VF089_13255"
     CDS             complement(2104..>5354)
                     /locus_tag="VF089_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6174979.1"
                     /translation="FAQSEPHESILLLQLFIATISMTALIIASVVSQQKRLEQRLRIK
                     DAVSRILSEASTMTDAAPQIIKAICETATWDFGALWFIDRDSNVLTCADMWCLPAVDV
                     SGFAAVTREIKFAPGVGLPGRVWASATPAWITDVLKDNNFPRAPVAISVGFHGAVGFP
                     IKLDNEVFGVLEFFSRETKERDEEFQDMIANLGTQFGQFIERQRAEAALRESEARLQL
                     ALSAGQMGAWEWNISASKITWSSSLEVIHGLKRGSFGGTFEDFKKTIHPEDLESVLNQ
                     IHTTLETRSDYDAAYRIVHPDGQIRWVEALASLSYGVDGRPDKLAGVCMDITESRYQQ
                     EMLRQRTRSLEIINQVGNTLAAELDLKKIAQIVIDAGREISGAQFGGFFYNLADGAGE
                     SFTLYSISGAPYELVDKLMPRNQQLLGSRSAGGVLRIGDVLDDQRFTTTAPDDGLIQG
                     RLPVRSYLSVPVNSRSGDVIGRIVFGHPQPNLFTAEAESIVTAIAAQASVAFDNANLY
                     QTVQRRVEEFQKLIDTAPIGIAVATDPESKHIWGNPEFMRMVGTRADQNLSKTGAEQD
                     QLSFEIYRNGIKVPAEDLPMQRAAREGIDVFDEELEIEREDGTVIHELCRATPLRDEH
                     GNVRGCIGIFLNISDRKQADAALQRAKDDLARANEGLEKRIQLRTAELEMANAALFAE
                     REEEKRLEQQLRQAQKMESMGTLASGIAHDFNNILNIIKGYASLLPQSVEDPQISAEA
                     LQVIDETVERGAATVRQLLALARESNLQFEPIDLNEMLENLKTLLSGTLPKTIDIEVD
                     LKTGLPEVMADPNQLHQVFLNVCLNARDAMPQGGKLRLGTDIVAGVELRKTYAEAKEG
                     SYACVSLEDTGLGIDDAVKHRIFEPFFTTKAQGQGSGLGLAVAYGIIANHRGFIEVIS
                     QPEQGATFRIYLPLAETSLPKHESKISSRSEAQGNISTEGQLVLFVDDEKNQVKLMVT
                     YLESAGYRVLTALDGIEAVETFKQHKDQIAVVVLDLGLPKLNGWDVLKQMKMTDPTIK
                     PILASGYVSAEVESALDNGELSAVIFKPYKLEEIKNAVAAAAVERNADHILG"
BASE COUNT         1172 a         1445 c         1455 g         1282 t
ORIGIN      
        1 actggcccgt gcagaacgga gtgatcgcat ggaataaggt agttgatatc tccgatgttc
       61 ttacgggcaa agctaaaggc cgcagtgacg acgaccagat cattctctat aagaaccaag
      121 gcgggcaggg catcgtcgat atcgctctag ccaagaagtg ctacgatctt gcgaaacaac
      181 acggcaaggg tcacgagctt cggattgaag cgcgtgagaa ctggtgggtg cagggaggaa
      241 gaactcaaac gtggtagatt caaaggagac atagatatgc tatcgaaatc gggccgaata
      301 agtattccca aaacaatttt tcagaaagta aaagccttgc ttatgatagc cgtcgctgct
      361 gcgtttcttc ttggcacggg aactatcacg gcttctcacg ctcaggcacc ctattatcag
      421 ggaaaaacca tcacggttgt agtcggcacc gtggcagggg acctttacga cctttacgcg
      481 cgcgctttcg cgcagcacat gccgaagcat attccgggca accccaatat gatcgtgcag
      541 aatatgcccg gcgccggtca catgatcgct gccaattatc tttatagcgt ggccaagccg
      601 gatggcttaa ccatcggcgc tactttgccg accctttatt ttgagcaatt gattggccgg
      661 gcggaggtca agttcgactt ttccaaattt acatggctgg gcaatgctac gaagtcgccg
      721 caggtgctct atatgcgcgc cgacgcaccg tacaaaagca tcgaagatat acgaaatgcc
      781 aaagaggcgc ccaagtgcgg caccaccggc acaagcaaca tgggctactt tgtgcccaag
      841 cttctggaag agaccgtcgg cgccaagttc aatgtgatcg ccggatatcc gggcggcagc
      901 gaagtagacc tggcggtgga gaagggcgaa gtgcaatgcc gctcgctcag ctcggaagcg
      961 ttcttttcac gggaaccatt ttttacctgg cgtaaaaata acttcgtccg agtgctcatg
     1021 cacggcgcca aagaaccgga tgagagactg cccggggtcc cgagcatcta tgagctgatg
     1081 gataaataca aaacagcgga gtcggggcgg cgtttggcga ccgcactcat ggctgccggc
     1141 gaatttcatc gtccttatct tgctcctccc aacctgcgtc ccgagcacgt taagatatta
     1201 cgcgaggcat ttttgaagac cgcaaaagat ccggcatttc tggaagagac gaaaaagaaa
     1261 aagctcgatc tcgatcccag cagtggcgaa gaggtcgcga ctctggttaa ggaagtaatg
     1321 tcccagccgg cggatgtggt cgaacgactg aagaagctaa tggggcgtta gaggtcgtag
     1381 actacgccac taatcttgca gcttgcatga tgggggctcg gcgcagctac taactagcgc
     1441 tctggctgcc gcgctcaagg ttgctaccgc tcctcccgct ccctacagtt ttgctttcgc
     1501 tttcttctct tccggcgctt tgatcttatc gagattctgc gccgcatagt cgccagtcgt
     1561 aaccacttcg tccttgtcgc tgaagcgcgg tttggcggcg gcctcggcgc gcgccccgag
     1621 aaggatcatg tactcgtcgc cggtgttgtc gagttgatag agttgaccgg cggggatcgt
     1681 cacaagcatc ccgggtgtga gttttcttgc cgggctatcg ggaaagtgaa acgtgcattc
     1741 gcccttgacg cagtagaatt gctgatcggc cgtgtgtttg tgcatcgggc ccttggttcc
     1801 cggataatcg ccatgcaccc aggtatgaaa gtgatcggat gtgaacagaa cttttttgct
     1861 gtggcctatg tccagttccg gcaggtttaa cagatctata aattccgcgg ccatggttag
     1921 tctcctattg aatttgcttt attgagtttg tttagtgttg aaatagcctc gtaggcttat
     1981 tgcgcttcgc cgcttcacgt tagcacctga aagcagagtt cttcatctgc tagagaaccc
     2041 ttcggtcatt actcgttgaa atggtgatat ggggagcaat actccagcct cgcaattcct
     2101 gcatcatccg agaatatggt ctgcgtttcg ttctacggca gccgcggcga cggcattttt
     2161 gatttcttcg agtttgtaag gcttgaaaat aactgcgctt agctcgccgt tgtccaacgc
     2221 agattcgacc tcagcggaaa cataaccgga agcgagaatt ggctttattg tcggatcggt
     2281 catcttcatt tgcttcagaa cgtcccaccc attcaattta gggagcccca gatccagaac
     2341 cacgactgcg atttggtcct tatgctgttt aaaagtctcg acagcctcga taccatcaag
     2401 cgcggttaat acgcgataac cggcggattc aagataggtg accatgagtt tgacctggtt
     2461 cttctcatcg tcgacaaata ggaccagctg gccttccgtc gaaatatttc cctgcgcttc
     2521 agaacggctc gaaatcttcg attcgtgctt tggcagcgag gtctcggcga gcggcagata
     2581 gatgcgaaaa gtagcgcctt gttctggctg gctgatgact tcgatgaaac cacgatgatt
     2641 ggctatgatc ccgtaagcga cagccagacc cagaccactg ccttggcctt gcgccttggt
     2701 tgtaaaaaag ggttcgaata tgcggtgctt cacggcgtcg tcgattccca atccggtatc
     2761 ttcaagagag acgcaggcat aggatccttc tttcgcttcg gcataggttt tccgaagttc
     2821 cacaccagca acgatgtcgg ttccgagacg aagctttccg ccctgcggca tggcatctcg
     2881 agcattgaga caaacgttca aaaacacctg gtgcagctgg ttgggatcgg ccatgacttc
     2941 gggcagaccg gtttttaggt ctacctctat gtcgatagtt ttcggcaggg tcccgctcag
     3001 cagggttttc aaattctcca acatctcatt gagatcgatg ggctcgaatt gaaggttgct
     3061 ctcgcgcgcc agcgcgagca gctgccgcac ggtggctgct ccgcgctcga ccgtctcgtc
     3121 gatgacttgc aaggcctctg ctgaaatttg cgggtcctct acggattgcg gcagcaggga
     3181 agcatacccc ttgatgatgt ttaggatgtt attaaaatca tgcgcgatgc cgctcgccag
     3241 agttcccatg ctttccatct tctgcgcttg tcgtaattgc tgttcgagcc tcttttcttc
     3301 ctcacgctcg gcaaaaagcg cagcgttggc catctctaat tctgcagtcc tcagttgaat
     3361 tcttttttcg aggccttcgt tggctctcgc taaatcatct ttggcgcgct gcagggctgc
     3421 atcagcttgc ttgcgatcgc tgatgttcaa aaagatgccg atgcagccgc gcacattgcc
     3481 gtgctcgtca cgcagaggag tcgcgcgaca aagctcgtgg atcaccgtcc cgtcttctcg
     3541 ctcgatctca agctcttcat cgaatacatc gatgccttcg cgcgcggctc gctgcatcgg
     3601 taagtcttcc gcggggacct tgatgccgtt gcggtaaatc tcaaacgaca gttgatcttg
     3661 ttctgcgccg gtcttcgata ggttctgatc ggcacgggta ccgaccattc gcatgaattc
     3721 cggattgccc cagatgtgtt tagactccgg atcggtagcc actgcaatac caatgggcgc
     3781 cgtatcaatt agcttttgaa attcctcgac gcggcgctga accgtctgat agagattagc
     3841 attatcgaat gcgaccgacg cctgtgcggc gatggcggtc acaatgctct ccgcctcggc
     3901 ggtaaaaaga ttaggctgag gatgaccgaa tactattcgg ccgataacat ctcccgaacg
     3961 agaatttacc ggaaccgaaa ggtaactgcg caccggcagg cggccctgga tcaggccgtc
     4021 atcaggcgcg gtcgttgtaa agcgctggtc atccaagaca tcgccgatgc gcagcacgcc
     4081 gccggcagat ctcgatccaa ggagctgttg gttgcgcggc atcaatttat cgacgagctc
     4141 gtagggcgcg ccggatatcg aatagagggt aaacgactca cccgccccgt cggcgaggtt
     4201 gtagaagaat ccgccgaatt gagcaccgga gatttcgcgt ccggcatcga tgacgatttg
     4261 cgcaatcttt ttcaggtcca gctccgccgc taaggtgttg ccgacttggt tgatgatctc
     4321 gagcgagcga gtccgctgcc gcagcatttc ttgttgatat ctgctttcgg taatatccat
     4381 gcacacgccg gccaacttat ccgggcgccc gtcgacgccg tacgacaaac tggcaagggc
     4441 ttcgacccag cgaatctgtc cgtccgggtg tacgatgcga taggcggcgt cgtagtcgct
     4501 gcgggtttcc aatgtcgtgt gaatctggtt cagaacagac tcgagatctt cggggtgaat
     4561 ggttttttta aaatcctcga aggttccgcc gaagctgccc cgcttcaggc catggatcac
     4621 ttctaaactc gaggaccagg tgatcttgct cgcgcttatg ttccattccc aagcgcccat
     4681 ctgtccggca gacaacgcca gctgaagtcg cgcttcactt tcgcgcagcg cggcttcagc
     4741 gcgctggcgt tcgatgaact ggccgaattg tgtgccgaga ttggcgatca tgtcctggaa
     4801 ctcttcgtcc cgttctttcg tttcgcggct aaaaaactcc agcacgccaa aaacttcgtt
     4861 gtcaagtttg atcggaaaac cgacggctcc atgaaagcct acgctgatcg ctaccggcgc
     4921 gcgcgggaaa ttattgtctt tgagcacatc ggtaatccaa gccggcgtag cgctggccca
     4981 cacccgtccc ggcaggccta cccccggagc gaacttgatc tcgcgggtga cggctgcaaa
     5041 ccccgacaca tcaacagcag gcaagcacca catgtcggcg cacgtgagta cgttcgaatc
     5101 gcggtcaatg aaccataatg cgccaaaatc ccaggtagcg gtttcacaga tggctttgat
     5161 gatctgcggc gccgcatcgg tcatcgtcga agcttcggat aggatgcggc tcacggcgtc
     5221 ttttatcctc aggcgttgct ccaaacgctt ctgttgcgag accaccgagg cgataatgag
     5281 agctgtcatg ctgatggtag cgataaacag ctgtagtaga agtatcgatt cgtgcggctc
     5341 cgactgcgcg aacg
//
LOCUS       DASUTG010000576         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1424562, whole genome shotgun sequence.
ACCESSION   DASUTG010000576 DASUTG010000000
VERSION     DASUTG010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424562"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF089_13260"
     CDS             complement(<1..423)
                     /locus_tag="VF089_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174980.1"
                     /translation="MRAAKYCGLILPAILVVVLGGALPRAHAETRISIGVTETMDTFN
                     PYADSVSLMYSVWCQVLGCLGTYDFDKGQPVGLLVDRWEVKDPKNWIFHLRKDIKFHN
                     GAPLTAEDVVHSINRIRTDPLSKQRQNIAAIASETVIDK"
     gene            complement(526..1509)
                     /locus_tag="VF089_13265"
     CDS             complement(526..1509)
                     /locus_tag="VF089_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174981.1"
                     /translation="MKPWITFLCSTVLFIASSNGASARDNLPIGYTAITGIKAGLWVA
                     EEAGIFEKYNIQPHLILITSGSKMVQAMLGGDLPLAGAAGNAAVDAALAGADIVMIGA
                     LAKVPAFYIMALPEIKSIEDLRGKAVGVTRFGSSTDFTIRYLLRKHGLNPDRDVTMIQ
                     AGDLFAAAAMLKTKAIVAAPFSSPTNLRAQEAGARVLMNMGEAGIYFPHDAWMARRSY
                     INANEDLIRRFMKGYSEGVAKLFSDPDLSRRAIRRFTRATDPKIVDAVYQYAVDYVEK
                     IPYNTREGIVEVLNQAAVRNPKAKEAKPEAFYDDRFVKELEMSGFYKQLYK"
     gene            1744..2784
                     /locus_tag="VF089_13270"
     CDS             1744..2784
                     /locus_tag="VF089_13270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6174982.1"
                     /translation="MHKRKLSRFAIQLLIVCLLGVPNILAAQDFYKDKIIRFIVGQAA
                     GGGYDTYTRTVARHMIKHIPGHPAITVENMTGAGSLVAANYIYHNAKPDGLTIANWNS
                     AFVLNQALGDHNVRFDARKFGWVGAPSKGVPVCLIMGFTGLKTFADIIKTDNPIKIGG
                     TAPGSHSIDLPLMLNKMLGTKFSVVSGYQGTAQIRLAMQRREVDGQCTNWDSIISTQR
                     DLLDAKGDDKMMPFLVHARLTDPELANVPLFSEVIKDPDHLITYQAYMAQMEYQRPLT
                     VAPGTPKDRLEILRRAFKATLGDADFVAEAKKAKLDVTYVSGEEIEKLVNEVLSISPK
                     IKENLQYLSQAK"
     gene            2821..3942
                     /locus_tag="VF089_13275"
     CDS             2821..3942
                     /locus_tag="VF089_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174983.1"
                     /translation="MAKSGFKVMDSDMHVFEPADLWQRYIDPKFADRAPVGLNRSFRD
                     LGIKVEGKVLPIPRNPENPALAKYRHDFFSEKYGDAGKRNFDSVSQIQAMNKEGLDVA
                     VLFPTRGLCVLGIDGLDPELAAAIAQAYNDWLHDFTQNAPDRMYGVAMVAPHDVRSAV
                     QELRRTVKEYGFRGIFIRPNHVNGRKWSDPYYDPLWAECEKLNLPVGFHEAGRVYLPQ
                     PAMFQFCSTFSMFNTFGFPFANMLACGDMIFGGVMERFPKLKVAFLEGNCSWAPWLLW
                     RMGEYADTTGKAEYPYLTLTPLEYFQRQCYGAVECDEITAKHIPEYGLEDNMVFSTDY
                     PHLDVKYPNAVDTFLELPFSESTKRKYLWDNCARMYNFD"
     gene            complement(4327..4632)
                     /locus_tag="VF089_13280"
     CDS             complement(4327..4632)
                     /locus_tag="VF089_13280"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6174984.1"
                     /translation="MLVVSNRIQVSQGFEKEFEERFAGRAGLVDKMPGFIRLEILRPI
                     KSDYYIVLTHWQDEASFRGWTDSKEFQEAHSNRPRAEIFAGPNVFEMHEVIQQSEKS"
     gene            complement(4906..5694)
                     /locus_tag="VF089_13285"
     CDS             complement(4906..5694)
                     /locus_tag="VF089_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174985.1"
                     /translation="MAYRIHPRFQLIGSFLVIIAAICIWPAIAAAETDKVPNEVVNQA
                     ASDGKVLVLVGLNVPWTMEGKLTENEIGTQRRAIGSAQNDLLFQLSGTEFRIVRVYDS
                     IPGIALEVGNDALAVLRKSKSVSNVLPDRPVIPAASPGEIEGTSTKTPRQDPPANTGL
                     VPTELFIEATNSGAVLVLVGLKTPWSPEGPLSKEMVAAQRGAIAAAQNYLLTELGDTD
                     YRITRRYRVIPGIALEVGLDALKVLARSVAVTNVLRDRPAISAR"
     gene            complement(5822..6739)
                     /locus_tag="VF089_13290"
     CDS             complement(5822..6739)
                     /locus_tag="VF089_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174986.1"
                     /translation="MRIAAVDLISNTCFPALAADELGFFKAEGLDARIELVASLGATK
                     ALRDGSADAMIAGSVHDVLTEFYQWEGVKIVVALSKGTPWLLVVRADLAAKRGDLNAL
                     KGLRLTAAEGPDLALRQMLIGAKIDPDRDLQIIELPGAKARDVSFGVFAARALEAGQI
                     DGFWANAMGAETAVSRGVGKVLVDVRRGDDPGEVRRFTFAGMVTSDSFTENNPAAVAA
                     AVRAIVKTQKALKADPRLAAEVGQRKFPREAAGLIGNVVERDVVFYDPTILPEDVEAM
                     NRFAQAVGHLSDPVAYDQVVATRFRDLWQ"
BASE COUNT         1623 a         1867 c         1783 g         1610 t
ORIGIN      
        1 cttatcgatg accgtctcgc tggcgatggc agcgatgttc tggcgctgct tgcttaacgg
       61 atcggtgcgg attcgattga ttgaatgcac gacatcctcg gcggtgagcg gcgcaccatt
      121 gtgaaatttg atatccttgc gcaggtgaaa gatccagttt ttgggatcct taacttccca
      181 acgatcaacc aataagccaa ccggctggcc tttatcgaaa tcataggtgc cgagacaacc
      241 caacacctga caccaaacgc tgtacatcag cgaaacactg tcagcgtagg ggttgaacgt
      301 gtccatggtt tcggtgacgc cgatagagat acgggtctcg gcatgagcgc gcggcaacgc
      361 gccaccgagt acaacaacca agatggccgg cagaatcaaa ccgcagtatt ttgctgccct
      421 cataaaccct ccatagaaca acaacaaaat cactgacgaa gccaatcaga actagcatcc
      481 gtgaatcgac ttcgctctgc gcgaatcaag tcgctcatgg ctcgcctatt tgtagagctg
      541 tttgtagaag cctgacattt ccaattcttt gacaaaccga tcgtcgtaga aagcttccgg
      601 ctttgcctcc ttggctttag gattacgcac cgctgcctga ttgagcactt cgacgatgcc
      661 ctcgcgcgtg ttatagggaa tcttctccac ataatccacg gcatattggt aaacagcatc
      721 cacaattttc ggatctgtgg cccgcgtaaa cctcctgatt gctcggcggc tgagatccgg
      781 atcgctaaaa agtttggcca ctccttcgga atatcccttc atgaagcggc ggatgagatc
      841 ctcgttggca ttgatatagg aacggcgcgc catccacgcg tcatgcggaa agtagatccc
      901 agcttctccc atattcatca atacacgagc ccccgcttct tgtgccctaa gattcgtcgg
      961 gctggaaaac ggcgctgcga caatggcctt ggtcttgagc atcgctgcgg cggcaaatag
     1021 gtcgcccgcc tgaatcatgg taacgtcgcg atccgggttt aagccgtgct tgcgcagaag
     1081 ataacggatc gtgaagtctg tcgaagaacc gaagcgggtg acgccaacgg ctttgccgcg
     1141 caggtcctcg atcgatttga tctccggcaa agccataata tagaaggcgg gcactttggc
     1201 gagcgcgccg atcatgacga tatccgcgcc agccagagca gcatccactg cggcattgcc
     1261 ggcggcgcct gcgaggggta aatcaccccc cagcattgcc tggaccattt tggaacccga
     1321 agtgatcaag ataaggtgtg gctggatgtt gtatttctcg aaaattccgg cctcttcggc
     1381 cacccacaac cccgctttga tgccagtaat cgcggtatag ccgatgggca gattatctct
     1441 agctgaagct ccattggatg aagcgataaa aaggacagtg ctacaaagaa atgtgatcca
     1501 tggtttcatg ctctctctcc tgttagtgca gttaccgctg tgctcggaga attccttgat
     1561 cgggagttaa ctatatcggt aaagcacctt aggcagggac cttatcaacc ggagaaaacg
     1621 gctgtcaaga tgaggcttga caaccgatcc tggggtcgac tatgaccctg gaaaatcgct
     1681 cgagatcaac ggcagctgct tttgtatatc gtaaaatctc tttcatgcgc aggaggagtt
     1741 tggatgcata agcgaaagtt gtcccgcttc gcgattcaac tgctcatcgt ctgtttgctc
     1801 ggcgtgccga acatcctcgc ggcgcaagac ttctacaaag acaagatcat tcgttttatt
     1861 gtcggacaag ccgccggcgg agggtacgac acttacacac gcaccgtcgc acgtcatatg
     1921 attaaacata tcccgggaca tccagcgatc acggtagaaa acatgaccgg agccggcagt
     1981 ttggtggcgg cgaattacat ttatcataat gccaaacccg atggtttgac gattgcgaac
     2041 tggaacagtg ccttcgttct aaatcaagct ctgggcgatc acaacgttcg cttcgatgcg
     2101 cgcaagttcg gctgggtcgg cgcgccgagt aaaggcgttc ccgtttgtct aattatggga
     2161 tttaccggcc tcaagacatt tgcggacatc atcaaaacag acaatccgat caaaatcggc
     2221 ggcaccgcac cgggttcgca cagtattgat ttaccgctga tgcttaacaa aatgctcgga
     2281 accaagttta gcgttgtctc tgggtaccag ggcacggcac agattcgcct agccatgcag
     2341 cgccgcgaag tggacggcca gtgcacgaac tgggactcga tcatctcgac ccaaagagat
     2401 ttgttggacg caaaaggtga cgacaaaatg atgccttttt tggttcatgc gcggcttacg
     2461 gatccggagt tggcgaacgt gccgctgttc agcgaggtca ttaaggatcc agatcattta
     2521 atcacttatc aagcctacat ggcgcagatg gagtatcagc gcccgctgac cgttgcccca
     2581 ggaaccccta aagaccggtt ggaaattctc cgccgcgcat ttaaggcgac tctgggcgac
     2641 gcggacttcg tagcggaggc taaaaaagcc aagttggacg taacttatgt gtccggcgag
     2701 gaaatcgaga aacttgttaa cgaggttctc tcgatatcac ccaagataaa agagaatctg
     2761 cagtaccttt cgcaggcaaa ataatcggcg cggcacgatc agcgaaatca aggagaggac
     2821 atggccaaaa gcggtttcaa agtgatggat tccgatatgc acgtgtttga gcctgccgat
     2881 ctctggcagc gttatatcga tccgaaattt gccgatcgcg cgccggttgg ccttaatcgc
     2941 tcgttccgag atttaggtat caaagtggaa ggcaaggttc tgccgattcc gcgaaatccc
     3001 gagaatccag ctctagcgaa ataccgtcat gatttttttt cggaaaagta tggcgatgcc
     3061 ggaaagcgaa acttcgacag tgtttctcaa attcaagcca tgaacaagga agggctcgac
     3121 gtggccgtcc ttttccccac tcgcggcctt tgcgttttag ggatcgatgg acttgatccg
     3181 gagttagctg cggcgattgc tcaggcctac aatgactggc tgcatgactt tactcagaat
     3241 gcgccggatc gcatgtacgg tgtcgccatg gtcgcgccgc atgatgtgag atcagccgtg
     3301 caggagcttc gacgcaccgt caaggaatac ggtttccgtg gtatctttat tcgccctaac
     3361 cacgtaaacg gtcgcaaatg gagcgatccc tattatgatc cgttgtgggc cgaatgcgaa
     3421 aagttgaatc tgcctgtcgg ctttcatgaa gcgggtagag tctacctgcc gcaacctgcc
     3481 atgttccagt tttgttccac cttttcgatg ttcaacacct tcggtttccc attcgccaat
     3541 atgcttgcct gtggcgacat gatcttcggc ggcgtgatgg agcgctttcc caaactgaag
     3601 gttgccttct tggaggggaa ttgctcctgg gcgccttggc tcctctggcg catgggcgaa
     3661 tacgcggaca ccacaggtaa agccgaatac ccgtatctca ctttgacgcc gctggaatac
     3721 tttcaacggc agtgctacgg cgcggtggaa tgcgacgaaa tcacggccaa gcacattccc
     3781 gaatatggtc ttgaggacaa catggtgttt tcgacagact atccgcacct cgatgtgaag
     3841 tatcccaacg ccgtcgatac ttttcttgaa ttgccgttct ccgaatcaac aaagcgcaaa
     3901 tacctgtggg acaactgcgc gcgaatgtat aactttgatt agcaaagagg acttgggtat
     3961 tgctaaccgg cattgaagag gatcggaagc gttgatcatt aaatcgcgaa actggctata
     4021 aacaatgctg gtggctattt tagactagcg aatgagaatg agatctccgt cagcggcttt
     4081 ggatgatgac tccgcacttc tcgcagcggt agcgggcaac gccgcttttt tcctccacca
     4141 attcctcgaa ctcgtattgc ttcaatcccg acgtgtcgaa acattccgga cagaagccat
     4201 ctttcaaatc ctcgcgggga agcgtcaaat gacagtggcg gcagcgcagt cgacttgcag
     4261 gctcaggcag caactctagc gtggtgtggg agcaggatga cattcttcca gttgtagacg
     4321 tctgatttag ctcttctcgg actgctggat gacttcgtgc atttcgaaga cattagggcc
     4381 ggcaaatatc tcggctcgcg gccggttgct gtgcgcttcc tgaaactctt tgctgtcggt
     4441 ccaccctcga aaggatgcct cgtcttgcca gtgggtgagc acgatgtagt agtcgctctt
     4501 gatcggccgc aaaatctcaa gccggatgaa ccccggcatt ttgtccacca gtcccgctct
     4561 accggcgaat cgttcttcaa attctttttc gaaaccctgg gagacctgaa ttctattcga
     4621 tactacgagc ataattccct ctactgtgcc aggaagttct cgaagagcac ttcgagttgt
     4681 caagaacata tgcctccgct gacctatcta tgcggagatg gaaattgaac gggccaactg
     4741 cctcagtcat agccgctttt tactgacttt tcaaaatctc ctgactagag ctgccgccgt
     4801 gttttcgtgt agattgttgg gtacggcgat ctgatacagc agaagggact ctgcgatcgc
     4861 gcaaaagaac ttgcctgcaa gagagtgcgg cccggaagaa gcctcctacc tcgcggaaat
     4921 agctggtcgg tcgcgcaaaa cgttagtgac ggctacggat cgggccagaa ctttcaacgc
     4981 atccagccct acttccaagg cgatgccagg aattacccga taacgccggg taattctata
     5041 gtcggtgtcg cctaattccg ttaaaagata attttgcgcc gcggcaatcg cacctctttg
     5101 cgcggccacc atctctttgc tgagcggccc ttccgggctc catggtgttt tcaagccaac
     5161 tagcacgagg actgcgccgg agttcgttgc ctcgatgaaa agctcggttg gaaccaatcc
     5221 agtattcgcg ggcgggtctt gacggggtgt cttcgtcgag gtcccttcga tttcccccgg
     5281 ggacgccgca gggataaccg ggcgatcagg caagacgttg ctgacacttt tggattttct
     5341 cagcaccgct agcgcatcat ttcctacttc caacgctatt ccggggatcg agtcataaac
     5401 gcgaactatc ctaaactctg tgccggataa ctggaaaagc agatcgttct gtgccgatcc
     5461 aatggcccgg cgctgggtgc cgatctcatt ttccgtaagc ttcccttcca tggtccaggg
     5521 gacgttcagg ccgacgagca caaggacctt cccgtcactc gccgcctgat tcacaacttc
     5581 atttggaact ttgtcggtct ccgccgccgc gatggcgggc cagatacaaa tcgcggcgat
     5641 gataactagg aacgatccta ttaattgaaa tcgggggtgg attcgataag ccatgatcgc
     5701 ttcctcgcac gcattgatac aaccgcaatc ggcttgagaa atactgcagt accgtaccgc
     5761 cgcgcaatca atttcacgcc tttgtatcgg cttcacgcca agacgggggg ctcgcgtcaa
     5821 cctactgcca cagatcccga aaacgcgtgg caacgacctg atcataagca acgggatcgg
     5881 ataggtgacc cacagcttgg gcaaatcggt tcatggcttc aacgtcttcg ggcaagatgg
     5941 tgggatcata aaataccaca tctcgttcga caacattccc tattaaaccc gcagcttctc
     6001 tgggaaattt ccgctgccca acttccgcgg caagcctggg atctgctttc aacgcctttt
     6061 gtgttttgac tatcgcgcgg actgcggcgg caacggcggc cgggttattt tcagtgaaac
     6121 tatcgctcgt caccattccc gcaaaagtga agcgccggac ttctccgggg tcgtcacccc
     6181 gccgcacatc gacgagaact ttgccaactc cgcgactaac ggcggtttca gcgcccatcg
     6241 cattggccca gaagccatcg atttgtcctg cttccaatgc ccgggcggca aacaccccga
     6301 atgagacgtc acgtgcttta gcgccgggca attcgataat ttgcagatct cgatcaggat
     6361 caatcttcgc gccgatcagc atttgccgaa gcgcaagatc gggaccttcg gcagccgtga
     6421 gacgaagacc cttgagtgcg ttgagatcgc cacgcttggc ggcaaggtca gcgcgcacga
     6481 cgagcagcca aggagtcccc ttcgaaagag ccacgactat cttgactcct tcccactgat
     6541 agaactccgt caacacatcg tgaaccgagc cagcgatcat tgcatccgca ctaccatcgc
     6601 gtaacgcttt agtggcgccc aacgacgcca ccaactcgat tcgggcatcc agcccctccg
     6661 ccttgaagaa acctagctca tctgcggcta acgcggggaa acaggtattg ctgatcagat
     6721 cgactgctgc tatgcgcatc tgcaagatgt cctcctaaaa gtctaaggaa aaatcttgcc
     6781 gaaactagca tattctctca tggatcaata gtcgaattgt atgctcgata tcctgttgga
     6841 cttggaaggc ggaaatcggg ctaaatggtg gtgctttgcc ggt
//
LOCUS       DASUTG010000577        11086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_830514, whole genome shotgun sequence.
ACCESSION   DASUTG010000577 DASUTG010000000
VERSION     DASUTG010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830514"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1115)
                     /locus_tag="VF089_13295"
     CDS             complement(135..1115)
                     /locus_tag="VF089_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174987.1"
                     /translation="MVIHLGIALLAIMLAASTSVAQEKAKIYLGASSKTLGYSPLWVA
                     TKKGFFEQQGLDVQLVLLRGMPMTVQALAAGSLHFGSGGPEPYIEAAERGLDFVITGG
                     VINGTAQFIIAGKNYKSYEDLRGATFGTASLTGGTITALREALKVKGLEYPRDYKLLI
                     VAGGSSANLAALQSGQIAATTVAVPLNYAAEEAGFNIIGRIIDGVPNFQTNVIATRRS
                     WAEKNRPVMVRFMKGVVQALRWLHDNKEPAIEFLSREIQLKPTHARKGWEFYTQNQLW
                     PVDGAVTLEGMKYNIRIYADQTGMKGPLPDPAKFVDQSYLSEALRELPKQ"
     gene            1386..2231
                     /locus_tag="VF089_13300"
     CDS             1386..2231
                     /locus_tag="VF089_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX6174988.1"
                     /translation="MTNKTVALLHPGNMGTTIGVAAATSGARMVWVSEGRSQATQERA
                     REARLRDVKTLTNAVNESDVILSVCPPDAALDLARNVAQHRFKGIYVDANAISRATAQ
                     QICEIVIQAGADFVDGGIIGSPVKRPGTTRMYLSGEHAPEIAALFSDSMLDAKTIGAE
                     PGAASALKVAYAAWTKCTDALLLAIRTFAASEGVDRALLQEWAISQPDLDRRSQQAAA
                     VATPKAWRYVGEMKEIAATFEAAGLPTGFHNAAAEIWERLAPFKDQTEPLPTITDVID
                     SLRAH"
     gene            2373..3389
                     /locus_tag="VF089_13305"
     CDS             2373..3389
                     /locus_tag="VF089_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6174989.1"
                     /translation="MNRRRNYPISTLVSFLGALFLLGTAYAQESLDQLHKKALQEGGT
                     LTFYGTLAQINAEKVLPAFEKRFPGLKVNHVDATADKLIARAITEARGGKTFVDVFQL
                     NLENLGQANDQGLLVAQVPPEAAAYPANLKGSYYVATDLQFIIAAWNTNLVKKEEEPK
                     QFEDFTQARWKGRLIAEPRDVELLVGLKQKHGSEEKAVAILKQIAANNVEFHKGHSEL
                     AEFLVAGQGALCVTCYAHHFPTRMKRGAPVNFMLTEGMGNVIAVAMAKNAPHPSTALL
                     WYRWVLTEEGQKAMAVGGRIPAHPKIEPTDKVRPEKIYPLTVADYKEFPKYNKLWKEI
                     FKLR"
     gene            3619..4602
                     /locus_tag="VF089_13310"
     CDS             3619..4602
                     /locus_tag="VF089_13310"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6174990.1"
                     /translation="MGLMVHGEWQDDDAIPSDARGHFIRAASRFRHWITADGAAGPSG
                     DDGFKAEPGRYHLFVSYNCPWAHRAIIMRKLKKLEDVVSMSLADRPKSEGWSYSRGID
                     DLQPSLDSTFRLHQLYKAADADYSGKVTVPTLWDRERRTIVNNESSEIIRMLNSAFAK
                     WTDVDYDFYPEELRHEVDTINDFVYEHLNNGVYRTGFARSQEAYDEAARKVFHGLDTL
                     NQRLGKTRYLAGQGITEADWRAFPTLLRFDLVYYSHFKCNLRRIQDYRHLVDYLRELY
                     QWPGIKETFDLEKIKAGYYSQRNINPTGIVPIGPDVTHLEQPHNRGPHVSI"
     gene            4753..5280
                     /locus_tag="VF089_13315"
     CDS             4753..5280
                     /locus_tag="VF089_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX6174991.1"
                     /translation="MKKVRSFIVGSAILTVATGAVMSSPLATFAQNESAGKGGFAHRA
                     RMHRMSGAGAPLISIALKHRTELNLSNEQVAGLEKIRTHYQDQSAPLQQQLRSIEGEI
                     SQLRQQSPANLIQIREKIEQSEKLRSELRYLREEALENGKSILTAAQKDQLKGLVSSE
                     HRGFRKRHGQTPKQS"
     gene            complement(5353..5919)
                     /locus_tag="VF089_13320"
     CDS             complement(5353..5919)
                     /locus_tag="VF089_13320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174992.1"
                     /translation="MKHLVNRVFITALVLGFCVPAWAQDIPFVPSPMIVVDRMLELAE
                     VKTDDIIYDLGSGDGRILIQAAKKYGARGVGIDMNPKLVADASRKAAEEKVGHLVQFR
                     AADGLTVDISDATVVTLYMFKWFNNQMRPKLQRLKPGARVVAHDFGIDEWEPNRVENV
                     DPKSDPSGELTNERTLYLWKVGTPAQIP"
     gene            complement(5945..7045)
                     /locus_tag="VF089_13325"
     CDS             complement(5945..7045)
                     /locus_tag="VF089_13325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6174993.1"
                     /translation="MASTATDLIIDADAHVVESDHTWDYLDPSDRQYRPQPLESREDA
                     GVKLQFWLIDGKVRGFRFPAFSAEELERRSHQVGRKFADVKESREMGNVDLRLEYMDQ
                     TGVDIQVLHNTMFIESATERAPVEVALCKSWNRWLADIWRQGKGRLRWSCMAPTLSIT
                     DALDQIRFAKENGACAVLMRPVEGTRLLADPYFYPIYDEAQKLDMAIAVHIANGSAWL
                     NDLYRHPVPIAATLHRFRIPTVAAFNDVLLSEIPHQFPKLRWGFVEASSQWLPWVLHE
                     AKNRFRTLGREWQGNIAREYRMFVTCENSDDLPYIVNEAGEDCLVVGTDYGHTDISSD
                     IDAIKIFRQRTDLTENVKRKILSDNARALYAI"
     gene            complement(7313..8137)
                     /locus_tag="VF089_13330"
     CDS             complement(7313..8137)
                     /locus_tag="VF089_13330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174994.1"
                     /translation="MMELSRRIRPLLLTLCLSVFAACAGAQPQAQNQSYEPRIGQQGK
                     DVVWVPTPQELVDKMLDMAKVTPKDYLIDLGSGDGRTVITAARRGLRAHGIEYNPDMV
                     ELSKKNAAKAGLTDKATFAKADLFESDFSDAQVITMFLLPQINLKLRPKILDLKPGTR
                     IVSNSFTMGEWEADESATVGGECDHWCTALLWIVPAKVEGTWQLPQGELMLKQTFQML
                     SGTLKTADSSSAITNAKMIGDQIVFTAGGNQYTGRVNGNVMEGKSGSGSWKATRTK"
     gene            complement(8187..9011)
                     /locus_tag="VF089_13335"
     CDS             complement(8187..9011)
                     /locus_tag="VF089_13335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6174995.1"
                     /translation="MNAAHICCRLFLAVFIAVLSAGAAVQAQPAKQEFKPEVGQEGKD
                     VIWVPTPQELVEKMLDMAKVTPKDYVIDLGSGDGRTVITAARRGAKALGIEYNPDMVE
                     LSKRNAAKEGVSDRATFMKADLFEADLSQAQVITMFLLPDINLKLRPKLLNLRPGTRI
                     VSNSFTMGEWQADEIANVKNGCASYCTAYLWIVPAKVDGTWQLPQGELVLKQEYQMVS
                     GTLKTDNGAAPIKGKVIGDQIRFTAGGTEYQGRISGSTIEGSTRTGGSFKASRAVK"
     gene            9217..10371
                     /locus_tag="VF089_13340"
     CDS             9217..10371
                     /locus_tag="VF089_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6174996.1"
                     /translation="MRQRSDYPTFSDQEIATRHQSVYRLMEQENVDALLVYGSGRYAS
                     DVYWLSDWPSSREAYVLFQTGKEPVILLQLFNHFPMAKVMSVIKDVRWAGANTTNSVL
                     DLIRERGLESKKIGLVGALSYQVYLKLVEKFPAAGFSDLSNKLRMMRTIRSPEEIERI
                     GFASKLTDESIEALAGGLKAGIREDEIAALIEPAYLKRGGYAGIHFMSSMPMRDPDFA
                     VPAQFQSSRKLQKGDCLITEISGAYSGYSGQIHRTFSIGEGPTPQWQKMHDAAVEAFH
                     VLAEVIKDGATTTEAEEAAEIIHRRGYSTYDDLVHGVNQYPPIFQTKTRKRHDSKEMV
                     FRENMVIVIQPNLITEDEKMGLQFGETLLVTKKGCETLNAYPREWVTCQA"
     gene            complement(10458..>11086)
                     /locus_tag="VF089_13345"
     CDS             complement(10458..>11086)
                     /locus_tag="VF089_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6174997.1"
                     /translation="YAGIHYIGASRGTTLRGAHPLFSTALAVAFLGEKATLPVIAGTV
                     LIVGGITLISWQTEAQRARFRWWHVSYPLGAAFLAGISHPLRRYALGLANEPLYLAAV
                     IGVVAFPWLASYVILPRKGAEQPVWNRQSMGAFLFAGVFETLGIVLVIAALSVGEVVI
                     VSPIVATSPLWILLGTWLFLRGIERLSLRTIAGAFSVVIGTIAISLVR"
BASE COUNT         2536 a         3124 c         3011 g         2415 t
ORIGIN      
        1 gccgggcgcg ggattggttc ctcagatctc cactctatta tcgccttcag ctaaacggcc
       61 tgcgacgctt ggctgaattt gctccgtagc cttcactgcg atgctgagta ggtaaagaac
      121 aaacaactga acgatcactg cttgggtaat tctctcagtg cctcagataa gtagctttga
      181 tcgacgaact tggcgggatc gggcaacggc cctttcatgc cggtttggtc ggcatagata
      241 cggatgttgt atttcattcc ttcgagcgtg accgcgccat cgaccggcca gagttgattc
      301 tgcgtataga actcccatcc tttgcgtgcg tgagtcggtt tgagctgaat ttctcgcgac
      361 aaaaactcga tggccggttc cttattgtca tgcagccagc gcagcgcctg aacaacccct
      421 ttcatgaagc gcaccataac cggccgattc ttttccgccc acgagcgtct ggttgcgata
      481 acgttagttt ggaaattggg aacaccgtcg atgatccgac cgattatgtt gaaacctgcc
      541 tcctccgccg cataattcag cggcacggcg acagttgtgg cggcgatctg cccggactgc
      601 agtgcggcca ggttggcgga agagccgccg gcaacgatta gcaacttata atcacgcgga
      661 tactcaagcc ccttgacctt caatgcttcg cgcaacgcgg tgatggtgcc gccggttaaa
      721 gacgccgtgc cgaaggtcgc gccgcgtaaa tcttcgtagc tcttgtagtt cttgccggca
      781 atgatgaatt gagcggtccc gttgatgact ccgccggtga tgacaaaatc gagtccgcgc
      841 tcggctgctt caatataagg ctccggccct cccgagccaa aatgcaacga acctgccgcc
      901 agggcctgga ctgtcatcgg cattccacgc aagagaacca gttgaacatc gaggccctgc
      961 tgctcaaaga aacctttctt ggtggcgacc cagagaggac tatagcccag cgtcttgctc
     1021 gatgcgccaa gatagatttt ggctttctct tgagccaccg aagtcgaggc cgccagcatg
     1081 atcgccagta gcgcgatacc aagatggatt accatgcaac ctcttgttaa ggagttatct
     1141 ccgtgagcag ccgagccatt gactcaagct tggcctaagc cattcctcgt ttacaacgaa
     1201 cggtagggtc agagcaagct gcggggcgat ggttgttaac cgtagagctc attcttgact
     1261 cgcatgctcg gcgtcgaata ggttggcttt attcacaatg ttagggcatt taagcaatcg
     1321 cgcatatcga tgacggattt ttataacggc tagcgcgttt tgtcagtgct ccggaggaac
     1381 gatttatgac gaataaaact gttgcgctgc ttcatcctgg caatatggga accaccatcg
     1441 gggtggccgc agcaactagc ggagctcgca tggtctgggt gtcagaaggg cgtagccagg
     1501 caacccagga gcgggcgcgc gaggcgcggc tgcgcgatgt caaaacatta accaacgcgg
     1561 ttaatgaaag cgatgtcatt ctttccgtct gcccgccgga tgccgctctc gatcttgccc
     1621 gcaacgtcgc gcagcaccgg ttcaaaggaa tctatgttga cgctaacgca atttcacgag
     1681 ctacagctca gcagatctgt gagattgtca tacaagccgg cgcggatttc gtggacggcg
     1741 gcatcatcgg ttcgcccgtc aaacgtccgg gaacaacgcg aatgtatctc tccggtgagc
     1801 acgccccgga aatcgccgcg ctgtttagtg atagcatgct ggacgccaag accatcggcg
     1861 ctgagccggg cgcggcttcc gccttgaaag tcgcttatgc cgcttggaca aaatgcacgg
     1921 atgcgctgct gctggcaatc cgaacctttg ccgcgagcga gggggtggac cgagcgctgc
     1981 tgcaggagtg ggccatctcg cagccggacc tcgaccgccg tagccaacaa gccgccgcag
     2041 tagcaacccc gaaggcgtgg cgctacgtcg gtgagatgaa agaaatcgcc gcaacgttcg
     2101 aagccgccgg cctgcctacg ggctttcaca acgccgccgc tgagatctgg gagcggctcg
     2161 cgccgttcaa agatcagacc gagccgcttc caacgatcac agacgtgatc gatagcttgc
     2221 gagcccactg aagagtcgtt gttctcgtag gtaagcggta gtggacactc tgaagcgcgt
     2281 gttgacgggg tttatctcaa aaggtagtgt gcggcagttt ccatgacaat ccttttgaaa
     2341 acgctggcgc gacatcaaca cggaggtcga cgatgaatcg tagacgcaat tatccaattt
     2401 caaccctcgt aagctttctc ggagcgcttt ttctgcttgg aacggcttat gcgcaagaga
     2461 gcctagatca attgcacaaa aaggcgcttc aggaaggcgg cacccttacc ttctatggga
     2521 cgttggcgca gatcaatgcc gagaaggttc ttccggcatt tgaaaagcgc ttcccgggtt
     2581 tgaaggtaaa ccacgttgac gccactgccg acaagctgat tgcccgcgcg attaccgaag
     2641 ctcgcggcgg gaaaactttt gtcgacgttt tccagctgaa tctcgagaac ttagggcaag
     2701 ccaacgatca gggcttgctg gtagcgcaag tgccgcctga agcggcggct tatccggcca
     2761 acttaaaagg gtcctactac gtagccaccg atcttcagtt catcatcgcc gcatggaata
     2821 ccaatctggt aaaaaaagaa gaagaaccga agcaatttga agatttcacc caagcgcgct
     2881 ggaaagggcg gctgatcgcc gaaccgcggg atgtcgagct tctggttggt ctgaagcaga
     2941 agcacggcag cgaagaaaag gccgtcgcta ttctcaaaca gatcgcggcc aacaacgtcg
     3001 agtttcataa gggtcattcg gagctcgccg agtttttggt cgccgggcag ggcgcactgt
     3061 gtgtcacctg ctatgcccat cattttccaa ccagaatgaa gcggggagcg ccggtgaact
     3121 tcatgcttac cgaaggcatg ggaaatgtca ttgcagtggc gatggcaaag aatgcgccgc
     3181 acccgagcac ggcgttgcta tggtatcgct gggtgctcac ggaggaagga caaaaagcta
     3241 tggctgtcgg cggccgcata cccgcccatc ctaagatcga gcctaccgac aaggtgcgac
     3301 ctgaaaagat ttaccccttg actgtcgctg actataagga gtttcccaag tacaacaaac
     3361 tttggaagga aattttcaag cttcgctagg ccgggacaaa caacgcgggc cgaacgggga
     3421 agtcgcgtat cggtaaaagc cggcggccag ttagtatggt agagtccccc tcgttcatcc
     3481 accccgccat ttcgcggggg aggaaagagg tgggggttca ttagcacgag caaacgcacg
     3541 aaagatctcc gcagagtaat tcatggcctc cgctgtttac cgcaactcgc gtcatagaca
     3601 cacaaggagt agctccttat gggcttaatg gtgcatggcg aatggcagga cgacgatgcg
     3661 atccccagtg acgcgcgcgg gcatttcatt cgcgcggctt ctcggttccg gcactggatc
     3721 accgcggatg gcgcagctgg gccgtccggc gatgacggct tcaaagccga acccggccgc
     3781 tatcatctat tcgtctctta taactgcccc tgggcgcatc gcgcgatcat catgcgcaag
     3841 ctcaagaagc tcgaagatgt cgtctcgatg tctctcgccg accgacccaa gagcgaaggc
     3901 tggtcctatt cccgcggaat agacgatctc cagccatctc tcgatagtac ttttcgcctg
     3961 caccagctgt ataaggcggc cgatgccgat tacagcggca aggtgaccgt gccaaccctg
     4021 tgggaccgcg agcgtcgcac catcgtcaac aacgaatcct cggaaattat tcgcatgcta
     4081 aattctgcgt tcgccaagtg gacggacgtg gactacgact tttatcccga ggaactgcgc
     4141 cacgaagtcg atacgatcaa cgattttgtt tatgaacact tgaacaacgg cgtttatcgc
     4201 accggcttcg ctcgaagcca ggaggcctac gatgaagcgg cgcgaaaagt atttcacggg
     4261 ctcgacaccc tgaaccagag actaggcaaa acccgttatc ttgcgggcca aggaatcacc
     4321 gaggcggact ggcgagcgtt tcccacgctg ctgcgcttcg acctggttta ctacagtcat
     4381 ttcaaatgca atctgcgccg gattcaggac tatcgtcatc tggtagatta tttgcgcgag
     4441 ctttatcagt ggccggggat caaagaaacc ttcgatctcg agaagatcaa ggctggatac
     4501 tacagccagc gaaacatcaa tccaaccggc atcgtgccga ttggacccga tgtaactcac
     4561 ctcgaacagc cgcacaaccg cggacctcac gtttcgattt agccacgacc aagcatcgtt
     4621 gcttatcttg gatctggccg aagaaaattc gtctatctac gggccaatcc ggcatcgatg
     4681 tcagtggtgt aaaccttccg gcgcggagcg cgtctaatca ttaaaattct ttcagcctga
     4741 ggaggggtct ctatgaaaaa ggtccgttca tttattgttg gcagtgccat cttaaccgtt
     4801 gccaccggcg cagtcatgtc atcgccgttg gccacgttcg ctcagaatga atcggcaggc
     4861 aaaggcggtt ttgcgcaccg cgcgcggatg caccgcatgt ctggcgccgg cgcgccgcta
     4921 atcagtatcg cgctcaagca tcgaaccgag ctgaatctct cgaacgagca agtagcgggt
     4981 ttggagaaga tcagaaccca ctaccaagac cagagtgctc cccttcaaca gcagctgcgc
     5041 agcatcgagg gcgaaatctc gcagttgcgc cagcaaagcc cggccaactt gattcagatt
     5101 agggaaaaga tcgaacagtc tgagaagctc cggtcggagt tgcgctacct gcgcgaagaa
     5161 gcgttagaga acggcaaatc gatcttaacg gcagcgcaga aagatcagct gaagggcctg
     5221 gtgagttccg agcatcgcgg gtttcgaaag agacacggcc agacgcctaa acaaagttaa
     5281 cagccgacct tacaaccaac aaagcccgag gaaaatcctc gggctttgtt gttatctcac
     5341 accgattcaa ttttagggta tctgagccgg cgttcccact ttccatagat acagtgtgcg
     5401 ttcattggtc aactcgcctg aaggatccga tttcggatca acattttcaa ctctgttcgg
     5461 ttcccattca tcaatgccga aatcgtgcgc gaccacccgc gcgcccggct ttagccgctg
     5521 caacttgggc cgcatctgat tgttgaacca cttgaacata tagagcgtga ccaccgtcgc
     5581 atcggaaata tcgacggtga gtccatcagc cgcgcggaac tgcaccaggt ggccaacctt
     5641 ttcctcggcg gctttgcggc tagcatccgc caccagcttc gggttcatat caatcccgac
     5701 tccgcgagcc ccgtactttt tcgccgcttg aatcagaata cggccatcac cgctgcccaa
     5761 gtcatagatt atgtcgtctg ttttcacctc ggcgagctcg agcatccggt cgaccactat
     5821 catcggcgac ggcacgaaag ggatatcctg cgcccaggcg ggaacgcaga aaccgagaac
     5881 caacgcagtt ataaaaactc tgttcaccaa gtgtttcatc gtaagtatct ctttccttga
     5941 agatttagat ggcatagagc gcccgggcat tgtcactgag gattttacgt ttcacgttct
     6001 ctgtcaaatc ggtccgctgc ctaaagatct tgatcgcatc gatgtcgctc gatatgtcgg
     6061 tgtggccata atccgttcca actaccaagc agtcctcgcc cgcctcatta acgatatacg
     6121 gcaaatcgtc ggagttttca caggtcacga acatacggta ttcgcgcgcg atattgcctt
     6181 gccattcgcg ccctaaagtc ctgaagcgat tcttggcttc gtgcaacacc caaggcaacc
     6241 actgcgagct ggcttcgaca aagccccatc gcagcttggg aaactggtgc gggatttcgc
     6301 tgagcagcac atcattgaac gcggccaccg tcgggattct aaatcgatgc agggtagcgg
     6361 caatcggtac agggtggcga tagagatcgt tcagccaggc gctaccgttt gcgatgtgaa
     6421 cggcaatagc catgtcgagc ttttgcgcct cgtcatagat cgggtagaaa tatggatccg
     6481 ccagcaagcg tgtgccttcc accggccgca tcagtaccgc gcacgcgccg ttctccttgg
     6541 caaagcgaat ctgatccagc gcatcggtga tgctcagggt cggcgccata caggaccagc
     6601 gcaggcgacc tttgccttgg cgccaaatat cagccagcca gcggttccaa ctcttgcata
     6661 aagcaacctc caccggcgcg cgttcggtcg cggactctat aaacatcgtg ttgtgcagca
     6721 cctgaatatc gactccggtt tggtccatgt attccaagcg caagtcgacg ttgcccattt
     6781 cacgagactc tttaacgtcc gcaaatttac gaccgacctg atgagagcgc cgttccagct
     6841 cttcggcaga aaaagccggg aagcgaaagc ctctcacttt tccatcgatg agccagaatt
     6901 gcagcttgac cccggcgtct tcgcggctct ccagaggttg cggtcgatac tgccggtctg
     6961 atggatcaag gtaatcccag gtatgatccg actcgacgac atgagcatcc gcatcgatga
     7021 tcagatccgt cgcagtgctg gccatgctcg ttcctccgca ggtatattaa tcttctgaac
     7081 attatgaatg ctgtgagggc atgtcaaccc gactcagcgg gcgccgccgt gaataaatgt
     7141 aagaaccagc ggccgatttc ctccaccgca attcgggatg aagcgcgatc ggtgtaattc
     7201 cccaacgcgg ttgaatttct ttttagtccg actttgaacg gttgaacagt tacactccgt
     7261 agcgttatct ggcgttgagc ttttgaacgt tgaaccgttg aacgggagtt atttatttag
     7321 tcctcgtcgc cttccaactt cccgaaccgc tcttcccttc cattacattg ccgttgaccc
     7381 ggccggtgta ctgattgccg cccgcagtaa aaacgatctg atcgccgatc atcttcgcat
     7441 tcgttattgc ggatgagctg tcagccgttt tgagcgtgcc ggagagcatc tggaaagtct
     7501 gcttgagcat aagctctccc tgcggcagct gccatgtgcc ttccactttg gccgggacga
     7561 tccagaggag cgcggtgcac cagtgatcgc actcccctcc cactgtagcg ctttcatccg
     7621 cttcccactc acccatggtg aatgagttcg agacgatccg cgtgcccggt ttgagatcga
     7681 gaatcttcgg ccggagtttt agatttatct gcggcagaag aaacatggtg atcacctgtg
     7741 cgtccgagaa atcgctctcg aacaagtcgg ccttggcgaa tgtcgcctta tcggtgagac
     7801 cggccttggc cgcgttcttc ttcgacagct cgaccatgtc aggattgtat tcgataccat
     7861 gcgcgcgcag cccgcgtctg gccgctgtga tcacggtgcg cccatcgccc gagcccaggt
     7921 caatcagata atccttgggc gtcactttgg ccatgtcgag catcttgtcg actaactctt
     7981 gaggcgtcgg cacccacacg acatctttac cctgctggcc gattctgggc tcatagcttt
     8041 ggttttgagc ctgcggctga gcaccggcac atgcagcgaa aaccgaaagg cacagtgtca
     8101 acagcaacgg gcgaatcctt cgtgacaact ccatcatgat ctcctgtgat tgaatagatc
     8161 gatcgctgcg atccgaacgt gattttttat tttacggcgc ggctggcctt gaaactgccg
     8221 ccggtcctgg tgcttccctc gatggtgcta ccgctaatcc ggccctggta ttccgtgccg
     8281 ccggcggtga atctgatctg atcgccgatg actttgcctt tgatcggcgc agcaccgttg
     8341 tcggttttca aggtgccgga gaccatctgg tattcttgct tgagcaccag ctcgccctgc
     8401 ggcagttgcc aggtgccgtc cactttggcg gggacgatcc agaggtaggc cgtgcaataa
     8461 gaggcgcaac catttttgac attggcaatc tcatcggctt gccattcgcc catggtaaac
     8521 gaattggaaa cgattcgcgt gccgggtcta agattaagca gctttggccg tagttttaaa
     8581 ttgatgtcgg gcaacaagaa catcgtgatg acttgcgcct gagagagatc ggcttcgaac
     8641 aaatccgctt tcatgaaagt ggcgcggtcg ctaacacctt ccttggccgc attgcgcttg
     8701 gacagctcga ccatgtcagg gttgtattcg atgccgagcg ccttggcgcc gcgcctggcc
     8761 gccgtgatta cggtgcggcc atcgcccgag ccgagatcga tcacgtagtc cttcggcgtg
     8821 accttggcca tgtcgagcat cttttccacc agctcctgcg gcgtcggcac ccagatcacg
     8881 tctttgcctt cttggccgac ttcgggttta aactcctgtt tggccggctg cgcctgcaca
     8941 gccgcaccgg cgctcagcac ggcaataaat accgccaaaa acaggcgaca acaaatatgc
     9001 gctgcgttca tgtggctctc ctattttgtc tagcgaattt cgaactggag agtcgcacgc
     9061 gcagaagaaa atgtctaggt tgaccggcac tgatcgacgc atgcagcgcc atcacttgcg
     9121 tcaatttgat tccctgaatc attcgatata aaaattcttc gaacgggaaa cgccggttca
     9181 tcaactcaaa cacagacccc gacaggagga tttcaaatgc ggcaacgatc cgattacccg
     9241 acattttccg atcaggagat cgccaccagg catcaatcag tttaccgcct gatggaacaa
     9301 gagaatgtcg atgcgcttct ggtttatggc agcgggcgct acgcttcgga cgtttactgg
     9361 ttgagcgact ggccgagcag tcgtgaagcc tacgtgcttt ttcaaaccgg caaagaaccc
     9421 gtgattctgc ttcaattgtt caatcatttt ccgatggcca aagtgatgtc cgtgattaag
     9481 gacgtgcgct gggcgggagc gaacacgacc aacagcgtac tcgatctcat ccgcgagcgc
     9541 ggcttggaat caaagaaaat cggtctcgtc ggcgcgctct cctatcaggt ctacttgaag
     9601 ctagttgaga aatttcccgc cgccggtttt agcgacctca gcaacaaatt gcggatgatg
     9661 cggaccatca ggagtccgga ggaaatcgag cgtatcggct ttgcgtccaa gctcaccgat
     9721 gagtctattg aagcgctggc cggcggcctg aaggcgggca tacgcgagga cgaaatcgcc
     9781 gcgcttatcg agccggctta tttaaagaga ggcggttacg ccggaattca ctttatgagc
     9841 agcatgccga tgcgcgatcc ggatttcgcg gtgccggcgc agtttcagtc gagccgcaag
     9901 ctgcaaaaag gtgattgcct gattaccgag atcagcggcg cctactcagg ttacagcggg
     9961 cagattcacc ggacattttc catcggcgag ggtcctaccc cgcagtggca gaagatgcat
    10021 gacgccgccg tcgaagcctt tcatgttcta gccgaggtga tcaaagacgg cgccacgacc
    10081 accgaagcgg aagaagcggc cgaaatcatt catcggcgcg gctacagcac ctatgatgat
    10141 ctggtccacg gcgtaaatca gtatccgccg atctttcaaa ccaagacccg gaagcgtcat
    10201 gattccaagg aaatggtgtt tcgcgaaaac atggtaatcg tcatccaacc gaacctcatc
    10261 accgaagatg aaaagatggg cctgcaattc ggcgaaactt tgctggtgac gaaaaagggt
    10321 tgcgagactt tgaacgcgta tccgagagaa tgggtgacct gtcaggcgta actcgagggg
    10381 acgcgatgat cactgcccct ccttcatcct ctcccgcgag gacgcgcggg aggaaagagg
    10441 tgggggaagt ttctccccta acgcaccagc gatatcgcaa tcgttccgat gacgacagag
    10501 aatgcgccgg cgatggtgcg caggctcaag cgctcgatgc cgcgcaggaa cagccaggtg
    10561 cccaataaga tccacaatgg actggtggcg acgatcggtg agacgatgac cacctcgccc
    10621 acactcagag cggcaatcac cagaacgatt cccaaagttt caaacacgcc ggcaaacaaa
    10681 aacgcgccca tcgactgccg attccaaacc ggttgctctg cccctttcct gggcaaaatt
    10741 acgtaactag caagccaggg gaatgcaacg acaccgatta cggcggcgag atacagcggt
    10801 tcgttggcca gacccaatgc gtaacggcgt agcggatggc tgatgccggc tagaaaagca
    10861 gcgcccagag gataactgac atgccaccag cgaaacctcg cgcgttgcgc ttctgtctgc
    10921 catgagatca atgtaatccc gccaacgatc aggacagtgc cggcgatcac cggcagagtc
    10981 gccttttcgc cgagaaaagc aactgccaga gctgtgctaa acagtggatg ggcgccccgc
    11041 aaggtcgtgc cgcgcgaagc gccgatgtaa tggataccgg catagg
//
LOCUS       DASUTG010000578         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_534314, whole genome shotgun sequence.
ACCESSION   DASUTG010000578 DASUTG010000000
VERSION     DASUTG010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534314"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..830)
                     /locus_tag="VF089_13350"
     CDS             complement(120..830)
                     /locus_tag="VF089_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174998.1"
                     /translation="MVQPRNASTVVLLRPADNDAFEILLTRRPLEMKFLGGYYVFPGG
                     AVHRDDYSSKVLERCRGLAAADARGILGMNGNANQALGHWVAVVRELFEEVGVLLCLT
                     ESGDEVDLRDPGVKDRIELKRQAIVKNELDFGAFLASENLLCDLSRAVYFDHWVTPEI
                     YSMRFDTRFYVAKLPSYQTPLAVSEEVIDSFWVAPQDALSQINRDDFPILPPTTTVLA
                     KLGRIGTWRGLHERFHLG"
     gene            complement(1063..1245)
                     /locus_tag="VF089_13355"
     CDS             complement(1063..1245)
                     /locus_tag="VF089_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6174999.1"
                     /translation="MFADYLLSPEGQKILGKFGLDSAVNKPPFKRYYAEAGMTVEQYE
                     KENAKWEKLLRDIGRK"
     gene            complement(1253..1951)
                     /locus_tag="VF089_13360"
     CDS             complement(1253..1951)
                     /locus_tag="VF089_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175000.1"
                     /translation="MMWYTSLTGGPNQEVPKAFEAKYPGIKVDVYRGDSDAIIQRVLQ
                     EAQAKRNLVDTIETTFPILKVMQEYKLLTPYFSPHLASYPDEVKEKAEKGLVYWATDR
                     ESYIGLAYNTNVVPEKGALKNFDDLLQPELRGKIGFATSDTGNRVLGAMLATKGPEFI
                     PKLKAQILFFTESPAGRLLIWLSPASSDYLPPYFSAIRALRFQRARRSSGWRWISCRP
                     MPAEWRTCQRAAPP"
     gene            complement(2243..2695)
                     /locus_tag="VF089_13365"
     CDS             complement(2243..2695)
                     /locus_tag="VF089_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175001.1"
                     /translation="MRDKATVLEAGEGTLLSARGSAMFFKATRASTAGAFSLMERTLP
                     PGGRKPPPHVHTNCEEAFYVLDGEIEFSVGETTTVGQRDTFVLVPGGVAHTFGNAGTT
                     PARLLVIHAPAMDAYFEELQALWSGDIPPTQDAEMELMRRHGMEPAGT"
     gene            complement(2874..>3257)
                     /locus_tag="VF089_13370"
     CDS             complement(2874..>3257)
                     /locus_tag="VF089_13370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6175002.1"
                     /translation="FFNDPVPVPNPAEEAAKMAAAMRQRVEELTVRWRKLDYELDFGV
                     GIAQGYATIGAIGFEGRLDYGAIGTVTNLAARLCGEAKPGQILIPQRLLGTVEELVDA
                     EPVGELHLKGFHRPVTAYNILGLKM"
BASE COUNT          740 a          905 c          840 g          772 t
ORIGIN      
        1 atcatgatga ttcctccttt ttagattatc gtttgcgact cttttactat atccgaatgc
       61 gagacctttt caatattgaa gcgactaaaa gcaggtgaag aagatgaggg tgatcggcat
      121 cagcccaagt ggaaacgctc gtgcaagcct cgccaagtac cgattcgccc aagcttggcc
      181 agcacagtcg tggtcggcgg cagaatcggg aaatcgtccc gattgatttg cgacaaggca
      241 tcctgtggcg caacccaaaa actgtcgatg acttcttccg aaacggccag gggcgtctga
      301 taggacggca gcttggctac ataaaagcgt gtgtcgaagc gcattgaata gatctccgga
      361 gtcacccagt gatcgaagta aaccgcccga gagagatcgc aaaggagatt ctccgacgcc
      421 aggaatgcac cgaaatccaa ttcgtttttg acgatcgcct gtcgcttgag ctcgattcga
      481 tctttgactc ctgggtcgcg caaatcgacc tcatctccag attccgtcag acaaagaagc
      541 acgccgacct cttcaaagag ttcccggact accgcgaccc aatgacctag ggcctggttc
      601 gcattgccat tcatgccgag aattccccgg gcatcagcgg ccgccagccc gcggcatctt
      661 tctagtacct tggatgaata atcgtctcga tgaacggcgc cgccgggaaa gacgtaatag
      721 ccgccgagaa atttcatctc cagcggcctg cgggtcaaga gaatttcaaa agcgtcgttg
      781 tcggcaggcc tgaggagaac gaccgtggag gcattgcggg gctgaaccat caaatcgctt
      841 ttatctcgag gtcaaaccgt cggttgttgc aatcacattt attgtaagcc tctacagtcc
      901 gtgtcattcc caaatgtttg tatcgggaat ccaggcgaga tcggaactgg acccccgatt
      961 aagacattcg gaggtgacag cttgggaata cattgtcctc cattatgttt ctacagccgg
     1021 ttgccaagaa tttattgcag agtgcgaggc cgatacctcg atttactttc tgccgatgtc
     1081 tcgcaacagc ttttcccatt ttgcattttc tttctcgtat tgctccaccg tcataccggc
     1141 ttcggcatag tagcgcttga acggcggctt gttgaccgcg ctgtcgagac cgaatttccc
     1201 caggattttc tgcccttcgg gacttaacaa gtaatcggcg aacaataacg cgctatgggg
     1261 gtgcggcgcg ttggcaggta cgccactccg ccggcattgg tcggcacgag atccatcgcc
     1321 acccacttga tcggcgcgcc ctttgaaatc gaagtgcgcg aatggctgag aaatacggtg
     1381 ggagataatc cgagctcgcc ggagacaacc atatcagcaa tcgcccggcc ggagactccg
     1441 tgaagaacaa tatctgagcc ttgagcttgg ggatgaactc tgggcctttg gtcgccagca
     1501 tagcgccgag cacccggttc cctgtgtcgg atgtggcaaa gccgattttg cctctgagct
     1561 ccggttggag cagatcgtca aaatttttaa gcgccccctt ttccggaacg acattggtgt
     1621 tataggcaag tccgatatac gattcgcgat cggtcgccca ataaaccaac cctttttccg
     1681 ccttttcttt gacttcgtcc ggatatgagg caagatgcgg cgagaagtat ggtgtaagca
     1741 gcttgtactc ctgcatgact ttcaagatgg gaaatgtcgt ttcgatggta tcgacgagat
     1801 tccgttttgc ctgcgcctct tgtagcactc tctggataat tgcatcgctg tcgcctcgat
     1861 aaacgtccac tttgattccg ggatacttcg cttcgaaggc tttcggcacc tcctggttgg
     1921 gtccgccggt gagcgacgtg taccacatca tccgcccttc tctttcgccc catcaaggag
     1981 catctgttca cggtcgggct tgttgtaagc ggcaagttcg gcaattgaca tgggctttcc
     2041 cgtctgggcc gaaactgcgg gcagccagac gagtgccgca aaaaaagaaa taagactctc
     2101 atgtcctctc cctcatcacg aaatggtcat gcaattaggc aacactgcta tgcactgaag
     2161 accaatcgac gagagccgaa tagaacttgc tccgcatgca gtgtttggca aggccgggac
     2221 gtacacggat cgtcacgaag acttacgttc ccgcaggctc catgccgtgc cgacgcatca
     2281 actccatctc ggcatcctgc gtgggaggaa tgtctccgga ccacagcgcc tgcagttctt
     2341 caaagtaagc gtccatcgcc ggcgcatgaa taaccaacaa acgggcgggt gtcgtgcctg
     2401 cattgccaaa tgtgtgagcc accccgccgg gcaccagcac gaatgtgtca cgctgcccaa
     2461 ctgttgtagt ttcgccgaca gagaactcta tctcaccgtc aagaacgtag aaagcttctt
     2521 cgcagtttgt gtgaacgtga ggcggcggtt ttctgcctcc cggtggcaag gtgcgctcca
     2581 tcaatgaaaa ggcaccggcc gtactcgccc gagtcgcctt gaagaacatt gcgctgccgc
     2641 gcgcagaaag cagcgtcccc tcgcccgcct cgagaacggt agccttgtct cgcataacaa
     2701 cttcctatga tatcaagatc cagaatttcg aaacccgaaa caaactgtca ttaaattgaa
     2761 ttcgaccaac atgccaagct acgcactcct taatcaggat cgttttgaac gttgtgttgc
     2821 ttatttcctt tttgaattcg tttcgaattt cggatttcga tattcgggtt gttctacatt
     2881 ttcaggccga gaatgttgta agccgtgacc ggacggtgaa agcctttgag gtggagttcg
     2941 ccaactggtt cagcgtcaac caattcttcc acggtgccga gcagccgctg gggaatcaag
     3001 atctgacccg gtttggcctc accgcacaaa cgtgcggcca gattcgtgac ggtgccgatg
     3061 gcgccatagt ccaagcgtcc ctcgaaccca atggcgccaa tcgtagcgta gccctgagcg
     3121 ataccgactc cgaaatccag ttcgtaatcc agtttgcgcc atctaaccgt cagttcttcc
     3181 acacgttggc gcatggcagc agccatcttc gctgcctctt ccgctgggtt gggcaccggc
     3241 actggatcgt tgaagaa
//
LOCUS       DASUTG010000579         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_684126, whole genome shotgun sequence.
ACCESSION   DASUTG010000579 DASUTG010000000
VERSION     DASUTG010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684126"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF089_13375"
     CDS             <1..409
                     /locus_tag="VF089_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6175003.1"
                     /translation="HGLSNEDLKHCQLLINIPADPEYQSLNVAQAAMICLYEIYLASL
                     GEPTKESLPLARAEDVERLYDRMRSSLLKIGFLDSQNPEHMPFAFRRILGRTRLEDRD
                     VRILTGLFRQIEWYAEQGWKVVQEKEKRGVKIR"
     gene            419..1057
                     /locus_tag="VF089_13380"
     CDS             419..1057
                     /locus_tag="VF089_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6175004.1"
                     /translation="MNVNHFEPKKTGLLFFDILNGYVPAPEPGKQRVLKPWIQNAVRL
                     SKAGRARGLPVFFAKGNHRPDNATTALLLTDTNNSLTPWPNGEVSKPKMHVIAGDRSS
                     DPLAELDPSPDDYYIVKYRWSAFYQTYLDLALRARGIDTLIISGGSTDVGVASTLYSG
                     RDMDYNMIVVSDACGTSHDQRAHDMLMELVFPRMARVRTTDQVIAMIEKAKA"
     gene            1264..2400
                     /locus_tag="VF089_13385"
     CDS             1264..2400
                     /locus_tag="VF089_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175005.1"
                     /translation="METNGKPPVGISKNIKQLGRMDIPGGGSIVVENGYAYVGHMDPP
                     HGTSIIDARDPKNLKVVAHLEIPEGLHSHKVRVSGDIMLINYERYKAKQELDSGLKIF
                     DISNKSKPREIAFFKTDGKGVHRFTFDGRYAYISPTIEGYQGNIAMVLDLKNPEKPEE
                     VCRWWMPGQWTAGGETPTWRGTDTRCHHPIRRDNRLYISYWHGGFAIVDISDMSKPKT
                     ISTLDWSPPYPCPTHTTLPMLDKIMERDWLIVTDEEVGEKLNETHNAFLWVVDITKEE
                     LPLPVATFIVPFEGKSTHQNRFGAHQPAEQVYGNTLYVTWFAGGLRAVDFSNPYIPKE
                     VGYYVPMPGKGQSVVMSNDVFHDKDGKLYLMDRYEGLEVLEPEI"
BASE COUNT          614 a          642 c          641 g          504 t
ORIGIN      
        1 ccacggcctc agtaacgaag atttaaagca ctgccagtta ctcatcaaca ttcccgccga
       61 tccggaatat cagtcattga acgtggcgca agccgcgatg atctgccttt atgagattta
      121 tctcgcttcg ctcggcgaac cgacgaaaga aagtttgccg ctggcgcgcg ccgaagatgt
      181 cgagcgtctt tatgaccgca tgcgctcatc gcttttgaaa atcggttttc ttgactcgca
      241 aaatcctgag cacatgccgt ttgcgttcag acgcattctg ggacgcaccc ggctggaaga
      301 tagagacgtg cgaatcctga ccgggctgtt tcggcagatc gaatggtatg cagagcaggg
      361 atggaaagtg gtacaagaga aagaaaagcg aggcgtcaaa attcgttagg aggcttaagt
      421 gaacgtcaac cattttgaac caaaaaagac cggactgcta ttcttcgaca tcttgaacgg
      481 ctatgttccc gcgcccgaac cgggcaagca gcgggtctta aaaccatgga ttcaaaatgc
      541 cgtgagactt agcaaagccg gccgcgccag gggtctgccg gtattttttg ccaagggcaa
      601 tcacaggcct gacaatgcca ccaccgcgct actccttacc gacaccaata actcgttaac
      661 tccttggccc aacggcgagg tgagcaaacc gaaaatgcat gttatcgccg gtgatagaag
      721 ctcagatccg cttgccgaac tggacccgag cccggatgat tactacatcg tgaaatatcg
      781 ctggagcgca ttttatcaaa cctacctcga tctggctctg cgcgcgcgcg gcatagatac
      841 gctgattatt tccggcggtt ccacggacgt cggcgttgct tcgactctat attccggaag
      901 agacatggac tataacatga tcgtcgtcag tgatgcttgc ggcacatccc atgatcagcg
      961 cgctcacgac atgctcatgg agctggtttt cccgcggatg gcgcgtgtgc gcaccaccga
     1021 ccaggtgatt gcgatgattg aaaaggcaaa ggcataagta cgacggctag cgttctgctc
     1081 ccggatgagt gatcagaaga ccgcgtcgct cgtcattcac gcgcacgcgg gaatcaactc
     1141 gcactcaatc tccccttttt aaggggagac aaagaggggt cctggattcc ggctcacgct
     1201 atctaccgtc agcttggccg gaatgacgta agaaataagc acgaagatat gaaggagaat
     1261 taaatggaga ccaatggaaa accgccggtg ggtatcagca agaacatcaa acaactcggc
     1321 cgaatggata ttccaggcgg aggatcgatc gtcgttgaaa acggctatgc ctatgtcggc
     1381 cacatggatc cgccgcatgg cacgtcgatc atcgatgcac gcgacccgaa gaatctcaag
     1441 gtggtagcgc acctggaaat tcctgaggga ctccactctc ataaggtgcg cgtgagcggc
     1501 gatatcatgc tgatcaacta tgaacgctat aaagcaaagc aagagctcga ttccggcttg
     1561 aagattttcg atatctcgaa taagtccaag ccgcgcgaaa ttgcattttt caaaaccgac
     1621 ggcaaaggcg tgcaccggtt cacgttcgac ggccgttatg cctacatctc gccgaccatt
     1681 gaagggtacc aaggcaacat cgccatggtc ctcgatctaa aaaatccgga aaagcccgaa
     1741 gaagtctgcc gctggtggat gccgggccag tggactgccg gcggcgaaac gccgacctgg
     1801 cgcggcaccg acacacgctg tcatcacccg atccgcaggg acaaccggct ctacatcagt
     1861 tactggcacg gtggcttcgc gattgtcgat atttccgaca tgagcaaacc gaagacgatc
     1921 agcaccctgg attggagccc gccttatcct tgcccgaccc acaccacctt gccgatgctc
     1981 gacaagatca tggagcgcga ctggttgatt gtcaccgacg aggaggtcgg cgaaaaacta
     2041 aacgaaaccc ataatgcatt tctctgggtg gttgatatca ccaaagaaga acttccgctg
     2101 ccagtggcga catttatcgt gcctttcgaa ggcaaatcga ctcatcagaa ccgattcggc
     2161 gcgcaccagc ctgccgagca ggtttatggc aatacattat atgtcacttg gttcgcaggg
     2221 ggtcttcggg cggtggattt ctccaatcct tacatcccga aagaagtagg ctattacgtg
     2281 ccgatgccgg gcaagggtca atcggtggtg atgagcaacg atgtttttca cgacaaagac
     2341 ggcaagctct atctcatgga ccggtatgag ggactggaag tactcgagcc ggagatatag
     2401 c
//
LOCUS       DASUTG010000580         3416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2017199, whole genome shotgun sequence.
ACCESSION   DASUTG010000580 DASUTG010000000
VERSION     DASUTG010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017199"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF089_13390"
     CDS             complement(<1..243)
                     /locus_tag="VF089_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175006.1"
                     /translation="MILKQSIGLGVVVFWCVMNGLLIKRQLWAPPPPLFVSVTESLTE
                     ASKEWWGVYYHGEKIGFTSQTIEPKQNGYELRDESLL"
     gene            304..780
                     /locus_tag="VF089_13395"
     CDS             304..780
                     /locus_tag="VF089_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX6175007.1"
                     /translation="MSKPTPPELRFAADVMLGRLAKWLRVLGQDVIYGRHLSGAGLTH
                     AARQENRIILTRDRRLHLKPSHDSLFIESDDFRQQLRQVVSTFHLVQSQDLFNRCLIC
                     NYSLHPRPKASVKQAVPEYVFSTQEHFFWCANCRRIYWPGTHHQKMLQELNNLQLK"
     gene            974..1049
                     /locus_tag="VF089_13400"
     tRNA            974..1049
                     /locus_tag="VF089_13400"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Asn,seq:gtt)
     gene            complement(1276..1836)
                     /locus_tag="VF089_13405"
     CDS             complement(1276..1836)
                     /locus_tag="VF089_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175008.1"
                     /translation="MNLSYRKKHNTDIWHFCSNCTNWPLTGFDKNDQGAPPLGESCPE
                     CVEHQRQGNCAQPKGTEASAPSDRKKILIIDDHPGVRRVLTITLRHLGYETCEASDAS
                     AGIRMSLLENPNLIVMDLSLPDSSGLETAKRIKENSQTSHIPIVACSGWKSDEILAQA
                     AEVGIVEFLAKPISPELLAAVIEKLT"
     gene            2106..2495
                     /locus_tag="VF089_13410"
     CDS             2106..2495
                     /locus_tag="VF089_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175009.1"
                     /translation="MNRDSIPNDLKQFILERIESVAYLEALLLLRSKPDAPWSCEAVA
                     NRLYIDQKQTAELLARLDADGFVTSAAEEEPMYRYQPATAHVQQMVDSLAEFYAKHLV
                     PITNLIHSKPKNRVQEFADAFRLRRDK"
     gene            2496..2759
                     /locus_tag="VF089_13415"
     CDS             2496..2759
                     /locus_tag="VF089_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX6175010.1"
                     /translation="MIAGFFYTLCTVTAFFCSCLLLRAYYRSRYKLLLWGGVCFLGLT
                     LNNGLLATDKLLLGPEVDLFTWRLVVALIAMMVLLYGIIWDVD"
     gene            2771..3061
                     /locus_tag="VF089_13420"
     CDS             2771..3061
                     /locus_tag="VF089_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX6175011.1"
                     /translation="MDFMLLGAIAMASLIAGLFFLRFWKDTGDRFFLFFAVSFLIEGV
                     NRAALGFSNDPNEGRPFFYFVRFLSFVLILIAIVQKNMIKDSPGKDSPMKRR"
     gene            complement(3148..>3416)
                     /locus_tag="VF089_13425"
     CDS             complement(3148..>3416)
                     /locus_tag="VF089_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175012.1"
                     /translation="LNGWEAYRKMKEVDPDIKAIFATGFIASQLETELAKQELSGVIM
                     KPYQLDEVLEKIHSAIKSKGLKPVRGTPSINNPASFSGTVKNHS"
BASE COUNT          798 a          863 c          851 g          904 t
ORIGIN      
        1 cagcagcgat tcatcgcgca actcataccc gttttgcttc ggctcgatag tctgcgaagt
       61 gaagccgatt ttctcgccgt gatagtacac cccccaccat tccttgctgg cttccgtaag
      121 agattccgtc acgctgacga aaagcggcgg cggcggcgcc cagagttggc gtttgatgag
      181 caggccgttc atcacgcacc agaacacaac gacaccgagg ccaatggatt gtttcaagat
      241 catgcggtag aacacggcta tggtaaatgc aaagggaaat cagttcaaac taaatgccag
      301 cagatgtcga aaccaactcc gccggaattg agattcgctg ccgacgtcat gcttggcagg
      361 cttgcgaaat ggctcagggt tttgggacaa gatgtgattt acgggcgaca tctctcgggc
      421 gccggtttaa cccatgcggc acgccaagaa aacaggatta ttctcacccg tgatcgccga
      481 ttgcatctga agccatcgca tgactctctt tttatcgaaa gcgatgactt ccgccaacag
      541 ctgcgtcagg tcgtcagcac gtttcatctg gttcaaagcc aggatctctt taaccgatgt
      601 cttatctgca attattcgct gcatccgcgc ccgaaagctt ccgtcaaaca agcggtccca
      661 gaatacgtct tttcaactca ggaacatttt ttttggtgtg ccaattgccg ccggatctac
      721 tggcccggaa ctcatcacca gaaaatgctg caggaactaa ataatctgca actaaagtag
      781 tcttaatcag tggattgaag cggcgagacc tcgccagact cgagttttgc cagacccagg
      841 atggtccctg cctggaattt gctggatccc tatgctaatt aaatgatctg ttcgcctcgg
      901 tcattcgaac aactcgcgat cagacgcagg gtgccgattg tacggcgcgg gcggatcaaa
      961 agttgtctat tattccccgg tagctcagtc ggtagagcag ttggctgtta accaattggt
     1021 cgggggttcg agtccctccc ggggagccag cttagggtat gacagaaagg cccttggaca
     1081 gatatcgagg gcctttctgc gtctctggcg ttgcgagcct gagagcctgc cattgctcat
     1141 tgatgcaagg atgaccttgc cttgcgaaat tatcttgagg cggacactaa accccgacgg
     1201 taccgaagtg ctcgatctaa cctgcgtgtc tgatgtaacc gcgttggtaa tgcagccggg
     1261 aaaagagttg tatcttcagg tgagtttttc gatcaccgcg gcaagtagct ccggcgagat
     1321 cggcttggct aaaaactcga cgatcccgac ctccgctgct tgtgcaagaa tctcgtcgct
     1381 tttccaaccg gaacatgcaa cgattgggat atgactcgtc tgcgaattct ccttgatcct
     1441 tttggcggtt tccaaaccgc tggagtcggg tagcgaaaga tccatcacga tcaaattggg
     1501 attctccaac aggctcattc ttattccggc gctggcatcg gatgcttcgc acgtctcata
     1561 acctagatga cgtaaggtga ttgtgagcac gcgccgaacg ccgggatgat catcaatgat
     1621 caggattttc ttgcgatcgc tgggtgcact tgcctctgtt cccttaggct gcgcacagtt
     1681 tccttggcgt tggtgctcga cacattcagg acaggattca cccaacggag gagcaccctg
     1741 gtcgtttttg tcaaaacccg taagcggcca attcgtacag ttggagcaga aatgccaaat
     1801 atccgtgttg tgtttcttgc ggtaagaaag gttcattgcc actggccgcc caattcgcaa
     1861 atgccgttcc ggcggatttg gcgatgcttg tccagaaatt tccgccgcac gacacttcac
     1921 atggccgttt gtaaaaggtg taagtgcctg taacgatcaa gcaacacttg aacagggcag
     1981 tgtcatgttg tcttcttagc aagctcaaac tgccaactga gtttcagtaa gccagctctt
     2041 ttgttgcagc acgctattac aacgcagccg gtcattcgct attaacttca gccaccgacc
     2101 accgcatgaa ccgagactca ataccgaacg atcttaaaca gtttattctg gagagaatcg
     2161 aatcggtggc ttatctagag gcgttactgc ttcttcgcag caaaccggat gctccatgga
     2221 gttgtgaagc tgtggccaat cgcctctaca tcgatcagaa acaaacggcc gagttactgg
     2281 cacgcctcga tgcagatggt ttcgtgacgt cagcggcgga ggaggaaccg atgtaccgat
     2341 accagccggc tacggctcat gtacaacaga tggtcgactc tttagccgaa ttctacgcca
     2401 agcatcttgt tccaatcaca aacctgattc actccaagcc caaaaacaga gttcaagagt
     2461 ttgccgatgc ttttagactc agaagggaca aatgaatgat cgccgggttc ttttacacgc
     2521 tgtgtacggt aaccgctttt ttctgttctt gtttgctgct gcgagcttat taccgcagcc
     2581 gctataagtt gctgctatgg ggcggtgtat gcttcttggg cttaacgctc aataacggac
     2641 tcttagccac agataaactg cttctaggac cggaggtgga tctttttact tggcgcttag
     2701 tggttgcgct gatcgcaatg atggttcttt tatatggaat tatttgggac gtggattgag
     2761 gagctgccgt atggatttca tgttgttggg cgcgatagcg atggcttcat taatagcggg
     2821 attgttcttc cttcgttttt ggaaagatac cggtgaccgc ttctttctgt tctttgctgt
     2881 ttctttttta attgaaggcg tcaatcgcgc cgcgctggga ttctccaatg atcccaacga
     2941 gggacgccca ttcttctatt ttgttcgatt tctctcgttt gtattgatcc tgattgccat
     3001 cgttcaaaaa aacatgatca aagattctcc cggcaaagac tcgccaatga agcgacggta
     3061 agggccaagc gcatgagctg ccgtgtggct cacctctaga gttagccagt ggattagctg
     3121 aatgcaccta aatttccagg caggcgctca ggaatgattc ttaacagtcc cggagaaaga
     3181 agcaggattg tttatagatg gagtgccccg aacaggtttc aaacctttgc ttttgatagc
     3241 gctgtggatt ttttctaaca cttcatcgag ctgatacggc ttcatgataa caccactcaa
     3301 ttcctgttta gccaattccg tttcaagctg agaggcgata aatcccgtgg cgaaaatcgc
     3361 cttgatgtca ggatcaactt ctttcatctt tctataagct tcccacccgt ttagtt
//
LOCUS       DASUTG010000581         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_387514, whole genome shotgun sequence.
ACCESSION   DASUTG010000581 DASUTG010000000
VERSION     DASUTG010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387514"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VF089_13430"
     CDS             <1..689
                     /locus_tag="VF089_13430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX6175013.1"
                     /translation="EPLVLFGYLAGLTKTIELVTGVLILPQRQTALVAKQAAALDIFT
                     GGRLRLGIGIGWNPVEYEALGENFSNRGQRSEEQVHLLRLLWTQDLVSFDGRWHKVTD
                     AGINPLPVQRPIPIWFGGGHDRVLRRVARLGDGWFPQLPPGEKCRAAIEKIATYAREA
                     GRNPKTIGIEGRMTISAESAETWHAQIEAWKKVGATHLSVNTMNAGFNKPTAHMDAIR
                     NFREATKQLW"
     gene            complement(704..1738)
                     /locus_tag="VF089_13435"
     CDS             complement(704..1738)
                     /locus_tag="VF089_13435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX6175014.1"
                     /translation="MLSGCSPIYFLRAAYEEGRILWRRQPITDFLATPDLHNDTKEKL
                     TTVLAVRDYAQNVLSLNVAGSYASYSYVDRPDLTYVVIAAPQTELRPYTWWFLVVGHV
                     PYKGFFSKSAAQSEAERLHKLGYDTTIRTSAAFSTLGWFDDPLLSHLLRFDKIALSEV
                     VFHELFHNTLYIKGAGAFNESAANFIGHRATIDFFGQRFGHGSAEHRRAVQLWEEELE
                     FAGFIEDLIVNLSGLYRRNITKEDKLRLREEVFARSKSEWARRIADQPAHRFRGFSQQ
                     PLNNAVLMHYGVYLNNLQLFESLYELEDRNLVRTIAALDQATQNSGEPFAALRSWLSK
                     HKIGALEQAG"
     gene            complement(1848..>2617)
                     /locus_tag="VF089_13440"
     CDS             complement(1848..>2617)
                     /locus_tag="VF089_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6175015.1"
                     /translation="VIFDDVWFHYKSDDPVLKGVSFRIEPGEKIAVVGATGSGKTTTI
                     KLLNRFYDVQKGSIQVSGVDVRDWDLQALRRHIGVVLQDVFLFSGDIRANLALGDRSV
                     PLERIENAACLANADTFIRRLAGGFASDVRERGSNFSGGQRQLLALARVLVFEPEILV
                     MDEATSSVDTETEALIQDALERVMRDRTCLLIAHRLSTIRNADRIIVLHRGEVREFGS
                     HSQLMEKQGIYHRLYQLQYEREELRLPVQEAEPKIYG"
BASE COUNT          625 a          726 c          704 g          562 t
ORIGIN      
        1 atgagccgtt ggttcttttc ggttatctgg caggactgac gaaaactatc gaactggtga
       61 ccggcgttct catattgccg cagcgacaaa ctgccttggt cgctaaacaa gcggcggctc
      121 tggacatatt caccggcggc cggttgcgcc tcggcattgg tatcggctgg aatcccgtcg
      181 agtatgaagc gctgggcgaa aattttagta atcgcgggca gcgctcggaa gaacaggttc
      241 acctgctgcg tttgctctgg acccaggacc tcgtcagctt cgatggtcgc tggcacaagg
      301 tcaccgatgc cgggatcaat ccattgccgg ttcaacggcc gattccgatt tggtttggcg
      361 gcggtcacga ccgggtgctt cggcgtgtag cacgtctcgg tgacggctgg tttccccaac
      421 tacctcccgg cgagaaatgc cgcgccgcga ttgagaaaat cgcaacttac gcacgcgagg
      481 ccggacgaaa cccgaagacc atcggcattg agggtcgaat gacgataagc gcggaatcgg
      541 ccgagacatg gcacgcccag attgaagctt ggaaaaaagt aggggcaacg caccttagtg
      601 tgaacaccat gaacgccggg tttaacaagc ctaccgctca tatggacgcg atccggaatt
      661 ttcgtgaggc aacaaaacag ctttggtgag ctctgtctct gcctcagccg gcttgctcga
      721 gcgccccaat cttatgcttg ctcagccatg aacggagggc cgcgaacggc tcgccgctat
      781 tttgcgtagc ttgatctaaa gcagcgattg tccgcacgag attacgatct tcgagctcgt
      841 acagagactc aaatagctgc aggttgttta gataaacgcc atagtgcatc agcaccgcat
      901 tattgagcgg ctgttgggaa aagccacgga aacgatgcgc cggttgatcc gcaatgcgtc
      961 gggcccattc ggatttgctg cgggcgaata cttcttcacg taaacgcagc ttatcttctt
     1021 tcgttatatt gcgccgatac agccctgaaa gattgacgat taggtcttcg atgaacccgg
     1081 caaattcaag ctcctcttcc cagagttgaa ccgcccttcg atgttccgcg cttccatggc
     1141 cgaatctttg accgaaaaaa tcgatggtcg ctcgatgtcc gatgaaattg gcggcgctct
     1201 cgttaaacgc gccggctcct tttatgtaca acgtattatg aaacagttcg tgaaaaacca
     1261 cctcgctaag ggcgatcttg tcgaatctga gcaggtgtga cagcagcgga tcgtcaaacc
     1321 agccgagcgt gctaaaggcc gccgaagttc ggatagtcgt gtcataccct aacttatgaa
     1381 gacgttcagc ttccgactgc gccgcacttt tggagaaaaa acctttgtac ggtacgtgac
     1441 cgacaactaa aaaccaccag gtatagggcc gcagctcggt ttggggagcg gcgatgacaa
     1501 cgtaagtcag atccgggcgg tcaacatacg aataacttgc gtaactgccg gccacgttaa
     1561 gactgagaac gttctgggcg taatcgcgca ctgccaaaac ggtagtgagc ttttctttgg
     1621 tatcattatg cagatccgga gtggccaaga aatcggtgat cggctgccgg cgccacaata
     1681 tccgtccttc ctcataggcg gcgcgcaaaa aatagattgg cgaacaaccc gagagcatcg
     1741 agctcaacat taatcccaca ataacggacc agaaacatgc gcgggtgata acgcgcccgg
     1801 ggcgaacaag ccttggtaat ccacgtaaga ctccccgacg ccggcaacta gccatagatc
     1861 ttgggctccg cttcctgaac aggcagcctc agctcctcgc gctcgtattg cagctgatac
     1921 aaccgatggt aaatcccttg tttctccata agttgtgaat gagaaccgaa ctcgcgcacc
     1981 tcgccacgat gaagcacgat gatacggtcg gcattgcgaa tagtcgagag ccggtgcgcg
     2041 atcaataaac aggtgcgatc gcgcataacc cgctccagcg cgtcctgtat caacgcttcg
     2101 gtctcggtgt cgacactgga ggtcgcttcg tccatcacca ggatttccgg ctcgaatacc
     2161 agcactcggg cgagtgcgag cagttggcgc tgtccaccgg aaaaattgct gccgcgctcc
     2221 cgtacgtcag aggcaaaacc tccggcaagc cgccgtatga aggtatcggc attggccagg
     2281 caagcggcat tttcaatgcg ctccagcggc accgaacggt caccgagcgc gagattggcg
     2341 cggatatccc cggagaaaag aaaaacgtcc tgtaaaacaa ccccgatatg tcttcgcagc
     2401 gcctgcagat cccagtcgcg gacatccacg ccgctaactt ggatcgatcc tttctgtacg
     2461 tcgtaaaaac gattgagcaa cttgatggtt gtcgtcttac ctgagcctgt cgctccgacg
     2521 actgcgatct tttctccggg ttcgatgcgg aaagacaccc cctttaaaac cgggtcgtcg
     2581 gacttatagt gaaaccagac atcatcaaag atgactt
//
LOCUS       DASUTG010000582         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_91749, whole genome shotgun sequence.
ACCESSION   DASUTG010000582 DASUTG010000000
VERSION     DASUTG010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91749"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..1997
                     /locus_tag="VF089_13445"
     CDS             621..1997
                     /locus_tag="VF089_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6175016.1"
                     /translation="MRPNPVYDRAVSDVACRFSGQLLNATDVEYEEARKVHNGLIDKR
                     PLLIARCRGVADVVDAVKLARALDLEVAIRGGGHNVAGRATIDGGLMIDLMPMKGIYV
                     DPQARTARAQGGVTWAEFNRETQVHGLAVTGGVVSSTGIAGLTLGGGLGWLMGKYGLA
                     LDNLRSVELVTAEGEVLRTNEEEHPDLFWAIRGGGGNFGIATSFEYELHPVGPTVTGG
                     LIAHPFERARDVLRFFRDTTASLPDDVTIFAGLIHSPDGEKLSAMLACHCGSLEDGEN
                     AMRPIKEFGSPLMDTIGLISYSDLNSMLDNGYPAGALNYWKSSFLKELSDDAIDAMIE
                     CFDACPTPMGQMLLEHFHGAATRIGVSDTAFPHRANGYNSLVLSQWMNPAENDRCIAW
                     ARQSYGQMEPFVGPGRYVNYLGDDEPGDPAVAAYGPNYSRLQEIKTKYDPTNFFHMNQ
                     NIRPLPKS"
     gene            complement(2066..2141)
                     /locus_tag="VF089_13450"
     tRNA            complement(2066..2141)
                     /locus_tag="VF089_13450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2105..2107),aa:Glu,seq:ttc)
     gene            complement(2324..2968)
                     /locus_tag="VF089_13455"
     CDS             complement(2324..2968)
                     /locus_tag="VF089_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175017.1"
                     /translation="MPGIRRISIHKLHLAVAILGLLILSTNTHAQRTPDAPGPVRQRE
                     ETRPASIEERQLKMMEIEREAKEPRSEAAQKLALAQIAQDYREIQIINNKMMGTSMPV
                     AKPNFLTIARTLEEIQKRAARLKENLRLAETKEKPEADKYKPAQNTKEFKTNLLALDD
                     SIMRLVKNPIFREPDVVNVDESAKARGELEFIIVTSQLIRKDADRLNKAADKSP"
BASE COUNT          716 a          777 c          855 g          763 t
ORIGIN      
        1 tggctgattc ttagtcaggg cgtttacggg caactctatt tctacggcca ggtccatatc
       61 ttgagccccg ttcgtcacct cttatgcgaa tttcctactg cccacttata tatacttccc
      121 gcggcggaga aacgttgaga aaaattaacg tcaccttcgc cgtcagtccg gaagaagaac
      181 gtgcggcgcg ccccattaag aaacgtttca tctgattcat gtcagttgaa atcgcgaaaa
      241 tgctgagggt gttggcgtcc caaagcattc cgagttggaa ccagattatt gtctggctgc
      301 gggagatgga aacacttcgc aaagcagccg cttagcctga cgtgctagta cactagtgcc
      361 ggattctcgt gccgggggaa gacacgcctg cttgattgga acctgaaaga ggagtagcac
      421 tcgcggatta gtggtgtgcc tgatgtgctt gatatagaat cggaacaaag agaatgctta
      481 tcctaagcag tgcatcttcc gtcctaaaaa ggccggttcg cggtcagagc tttccagtag
      541 caggctcgac gtgccggcgg ctagataagc cttaaggatc cttatcaatt tcgttcacac
      601 taacaccaag gagacaggtc atgagaccaa atcccgttta cgacagagct gtgagcgacg
      661 ttgcatgcag gttctctggt caactgctaa acgctacaga cgtggaatat gaggaagcac
      721 ggaaagttca caatggtctg atcgataagc ggccgttgct aattgcgcgt tgtcgtggag
      781 tagctgacgt agttgatgcc gttaagcttg ctcgagcgct ggatctcgag gtggcaattc
      841 gtggcggcgg ccataacgtc gcagggcggg caaccatcga tggcggactt atgatcgacc
      901 tgatgccgat gaaggggatt tacgtggatc cgcaggcccg cactgcacgt gcgcagggtg
      961 gtgttacttg ggccgagttc aatcgcgaaa cgcaggtgca tggtctggct gtcacgggag
     1021 gtgtcgtttc ttcaacaggg attgcgggac tcacgctcgg cggtgggttg ggctggctga
     1081 tgggaaagta cggcctggca ctcgataatc ttcgatcggt tgagcttgtt accgccgaag
     1141 gcgaggtgtt gcggacgaat gaagaggaac atcccgattt attttgggca attagaggtg
     1201 ggggagggaa cttcggcatc gcgacttcat ttgaatacga gcttcatcct gttgggccaa
     1261 cggtcactgg cggactgatc gcgcatccgt ttgaacgcgc tcgcgacgtg cttaggttct
     1321 tccgcgatac cacggcgtca ctccccgacg acgtcactat ttttgctgga ctaattcatt
     1381 cgcctgacgg agaaaagctg tcggcgatgc tcgcgtgtca ctgtggatcg cttgaggatg
     1441 gtgaaaacgc gatgcgccca attaaggaat tcggctcccc gctgatggat acgattggtc
     1501 tcatctctta cagtgacctc aacagcatgc tggacaatgg ttatcctgca ggggctctga
     1561 actattggaa atcgagcttt ctcaaggaat tgagcgacga tgcgatcgac gcgatgatcg
     1621 aatgtttcga cgcctgtccc acaccgatgg gtcagatgct gcttgaacat ttccatggcg
     1681 ctgcaacccg tatcggcgtc agcgacacgg cttttccgca ccgcgccaac ggctacaatt
     1741 ctctggtact ttcgcaatgg atgaatcctg cagagaatga tcggtgcata gcctgggcga
     1801 ggcaaagtta tggccagatg gagccgtttg ttggaccagg gaggtatgtg aattacctgg
     1861 gtgatgatga acctggcgat cctgccgtgg ccgcgtatgg tcccaactac agtcggctgc
     1921 aggagatcaa gactaagtac gacccaacca acttctttca catgaaccag aacattcgcc
     1981 cactgccgaa aagctagtat tgatcgataa gagataggcc caggatcttc ataacgacat
     2041 caacaaggcg agtaaccgag aaatctggcg tcccgtacgg gagtcgaacc cgtgtcgccg
     2101 ccgtgaaagg gcggtgtcct aggccactag acgaacggga cgcataaagg gaagttcgat
     2161 ggagccagga agccgacaaa cttaccgcac gggtatgaac gtgtcaaacg cgcgactgcg
     2221 acgactcaaa agtatcgtgt gtagattctg gccttgaagt taaaagcgat ccacgaagta
     2281 acacgaacga atacgagatc ggctttttct aaacaattca ctatcaagga ctcttatctg
     2341 ccgccttgtt aaggcgatca gcatccttgc gaatcagttg actggtaacg atgatgaact
     2401 cgagctctcc gcgcgccttg gccgactcat ctacgttgac cacatcaggc tccctgaaga
     2461 ttggattctt aaccagcctc ataatcgaat cgtcgagcgc taataggtta gtcttgaatt
     2521 ctttagtgtt ctgcgccggt ttgtatttgt ccgcctcagg tttctccttc gtttcggcaa
     2581 gacgaaggtt ttctttaagc ctcgccgcgc gtttctgaat ctcctccagt gtccgtgcaa
     2641 tcgtcagaaa gttgggcttc gcaaccggca tcgaagttcc catcatcttg ttattgatga
     2701 tctgaatctc acgatagtcc tgcgcgattt gggccaatgc aagcttctgt gctgcttccg
     2761 acctcggttc cttagcctcc ctttcgattt ccatcatttt caactggcgt tcttcaatgc
     2821 ttgccggtcg cgtttcttca cgctgtctta ctggccccgg agcatccgga gtacgttgcg
     2881 cgtgcgtgtt cgtggataag ataagtaaac ccaatattgc aacagcgaga tgaagcttgt
     2941 ggattgatat tctgcggatg ccaggcatgc cgttcctcct gaaagtgttt caggccaatg
     3001 agaatggagg acgcagtata ttccgcccta gcgcgggctt caatactcct gacgtactcg
     3061 gactgggcta acgcgagata tccggtttgt tcttagaaat gagcaccaaa g
//
LOCUS       DASUTG010000583         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1425844, whole genome shotgun sequence.
ACCESSION   DASUTG010000583 DASUTG010000000
VERSION     DASUTG010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425844"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..199)
                     /locus_tag="VF089_13460"
     CDS             complement(41..199)
                     /locus_tag="VF089_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175018.1"
                     /translation="MAICCAAPLLLVGAIAFLGLSLGARANGALSLAAVLVCPVGGFN
                     DPHDDENK"
     gene            complement(345..2567)
                     /locus_tag="VF089_13465"
     CDS             complement(345..2567)
                     /locus_tag="VF089_13465"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6175019.1"
                     /translation="MKKQKQTIDPIRYEMFLHRLWAIGEEGRMTLQRVTASPIVSQGG
                     ECMSSFYDQTGTMVLACSGHLRFAAATSDAIRNLMQRFSKTPGFHDGDQFFFNDPYVA
                     GSHTYDMMIVKPIFFRGELIAWTATSTHTADTGGVLRGGAYEIFHEGICIPGLKIVEK
                     NEFREDVFQSLTGQCRDPQYVGLDLKAMIAGNNICAKRYLGLVEKFGLKFVQLAGEKM
                     IADSESKARAKLRSMPDGTWVTRQYVTTLDRKTRKSVAQQLYCTMTKKGDELLFDFTG
                     TSPQTDTDHNSTLPSTVAHLALALTNTLFWDVPWSDGKMRPVKVHVPEGSILNCKYPA
                     ACGAGPRIGNVLVSTVCEAVAKMIYASGRYEDVNASTTGNLEFVGGPGYFYGGHTREG
                     IAVAQGLYDIHGAGMGARPHGDGVNTGGHMNIPSAGISDIERIEMQYPFLYFTRSHNP
                     DGSGFGQYRGGLGSYRIYLIYGSKDCSVDYKPYGGVAQGGFGLGGGYPTGIGATRVMI
                     EAGLEMLGRVKAGEYPTVAAMRAGEWGKSILPAGVPERVPLPEGTLLVDYVAGGGGFG
                     DPIDRDPAAVLKDFGYGWVSREIAERIYGVVFADGGKAVDSAATEDCRRRIREARKQD
                     APISKESSGKAAESSNEPRQLLRFHAALEIIGNGKQKTIRCSRCGHLYCKADENYKHY
                     ALHRILDLNNFMPPLPTGERYIGEYHIYSCPGCATQLQVDLYSPSLGGDPVLWDTRIA
                     "
BASE COUNT          552 a          762 c          703 g          589 t
ORIGIN      
        1 acatgtggtt taaattcaac tgttactgag aatcaattta ttatttattt tcatcatcat
       61 gcggatcatt aaaaccgccg accggacaga ccaataccgc cgcaagactc agagcgccat
      121 tcgctctcgc tcctaaagac aagcctaaaa aagcgattgc gcccaccaga agaagcggcg
      181 ccgcgcagca gattgccatg ccgagaccgc ctttcagcca gccgcttcaa acacctacgc
      241 gcttcgactc tctggaagct gtgtcgccct cttcgtacag accttttcgg ggtactagtc
      301 gcagacgcaa ctgctacgcg aaatattttt tcaggtgatt taacctaggc aattctcgta
      361 tcccacaaaa ccggatcgcc ccccaggcta gggctataca aatccacctg cagctgcgta
      421 gcgcagcccg ggcatgagta gatgtggtat tcaccgatat agcgctcgcc cgtgggcagc
      481 ggcggcataa agttgttgag atccaaaata cgatggagcg catagtgttt gtagttttca
      541 tcggccttgc agtataggtg cccgcagcgg ctgcaacgaa tggtcttttg cttgccattg
      601 ccaatgatct ccaaagccgc gtgaaaacgc agcagttgtc gcggctcatt actactttca
      661 gcggctttgc cgctggactc tttcgagatg ggagcgtctt gtttgcgtgc ttcgcgaatt
      721 cggcggcggc aatcttcggt ggcggcgctg tcgacagcct tcccgccatc ggcgaagaca
      781 acgccgtaaa ttctttcggc gatctcccgg ctgacccagc cgtagccgaa gtctttcaag
      841 acggcggccg gatcccgatc aatgggatcg ccaaatccac caccccccgc tacatagtcg
      901 accagcaaag tgccttccgg caacggcact cgctccggca ctcctgccgg taagattgat
      961 ttgccccact ccccggcgcg catcgctgct accgtgggat actcgcccgc tttgactcgg
     1021 cctaacattt ccagacccgc ttcgatcatc acccgggttg cgccgatccc ggtaggataa
     1081 cctccgccca ggccaaagcc gccctgggca acgccgccat acggtttgta atcgacgctg
     1141 caatctttcg aaccatagat cagatagatg cgatagctgc ccagcccgcc gcggtactga
     1201 ccgaagccgc tgccgtctgg attgtgcgac cgggtgaaat aaagaaacgg atactgcatt
     1261 tcgatgcgct cgatgtcgga aatgccggcc gatggaatat tcatgtggcc gccggtgtta
     1321 acaccgtcgc catgcggcct ggcgcccatt cctgctccat gaatatcgta aagtccttga
     1381 gcaacggcga tgccttcgcg cgtgtggccg ccgtagaagt atccggggcc gccgacaaat
     1441 tccaaatttc cggtggtcga tgcgttgacg tcctcgtacc gccccgaggc atagatcatt
     1501 ttggcgaccg cctcgcatac cgtcgatacg agcacgttgc cgatgcgcgg gccggcgccg
     1561 caggccgcgg gatatttgca attgagaatg gatccttccg gcacatgaac cttgaccgga
     1621 cgcatcttgc catcgctcca gggcacgtcc cagaaaagag tattggtcag cgccagcgcc
     1681 agatgagcca ccgtgctggg caaggtcgaa ttgtggtcgg tgtcggtttg cgggctcgtg
     1741 ccggtgaagt cgaacagcaa ctcgtcgcct ttcttggtca tagtacaata gagttgttgc
     1801 gccaccgatt tgcgcgtctt gcgatccaac gtcgtgacat attgccgggt cacccaggtg
     1861 ccgtcgggca tcgatctaag ttttgcgcgg gccttgctct cggaatcggc aatcattttt
     1921 tcgccggcca gctgcacgaa cttgagcccg aatttttcca ccaagcctag atatcgtttg
     1981 gcgcagatat tatttcccgc aatcatcgct ttgagatcga ggcccacgta ctgcggatca
     2041 cggcactgcc ccgtcaggct ttgaaacaca tcttcgcgga actcgttctt ctcgacgatt
     2101 ttcaagccgg gaatgcaaat accctcgtga aaaatctcgt aggcgccgcc gcggagtacg
     2161 ccgccggtgt cggcggtgtg cgtgctagtc gccgtccagg cgatcaactc gccgcggaag
     2221 aagatcggtt tgacaatcat catgtcgtaa gtgtgcgagc cggcgacata cggatcgttg
     2281 aaaaaaaact ggtcgccatc gtgaaagcca ggagttttgc tgaagcgctg catcagattg
     2341 cggatagcat ccgaagtggc ggcggcaaag cgcaggtgac ccgagcaggc aagcaccatg
     2401 gtgccggtct ggtcatagaa agagctcatg cactcacctc cttgagagac aatcggtgag
     2461 gcggtcaccc gttgcagtgt catgcgtcct tcttcaccga tggcccagag gcgatggaga
     2521 aacatttcat aacgaatcgg gtcgatggtc tgtttttgtt ttttcattgt tggctcctga
     2581 gttcatttct tcggaatgtc ggcgct
//
LOCUS       DASUTG010000584         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1275813, whole genome shotgun sequence.
ACCESSION   DASUTG010000584 DASUTG010000000
VERSION     DASUTG010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275813"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF089_13470"
     CDS             complement(<1..251)
                     /locus_tag="VF089_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175020.1"
                     /translation="MAKLRHLAILTHQPEKLADFYKRVFELKEMYRTKNGSVHLSDGE
                     VNLAILNASDPKEPGHAAGMYHFGFHVDDAEVVAQRIKEL"
     gene            452..724
                     /locus_tag="VF089_13475"
     CDS             452..724
                     /locus_tag="VF089_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX6175021.1"
                     /translation="MTYVIAEPCIDTKDTACVDVCPVDCIHPTKNETEEFEKENKLYI
                     DPEECIDCGACEPVCPVEAIFEEAAVPDKWKHFIKIDADWFKAKKG"
     gene            1000..2067
                     /locus_tag="VF089_13480"
     CDS             1000..2067
                     /locus_tag="VF089_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6175022.1"
                     /translation="MVLVLKNEQMEDLLPMAEEIDAIEEAFRELGAGQAMNAPRARLR
                     TPWKEEGGQYYFNNIMGLVPGVKSMALRIDSSFSKEVQIDGAKRRIYPGDFIGLVMLF
                     DMDSCELLAIMDDHHISAMRVAATSAVASKYLARQDSKVLGLLGSGEQAKTQVTAHLS
                     IRPLSKVKVYSPTKENREKFACDMSATTGIEVVPVASAEEAVRGSDIVSAATNTVDPV
                     VQGRWLEEGMHITSLVGGDGFLPRKELDDEVITRAALIVVGYKPQIFIDKQAEFHERL
                     ERGIVKAEDLHELGDLLNGKCRGRIDDKEITLFKNNTGMGIQFAATARKMYEKAREKG
                     VGSELPLDLFMTRRGDKTYSP"
     gene            2336..3043
                     /locus_tag="VF089_13485"
     CDS             2336..3043
                     /locus_tag="VF089_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6175023.1"
                     /translation="MREWEQIIPEEERRIYEKAGYKGKDSFGRNPALLVIDVITGFTG
                     TKPMPVLDAIDEFPTSCGQVAWDALPKIQQLLHACRDADVPVIYSTSDPDFKAAFGNA
                     TKRGVDATDFEKLAVAFPEMIKPKKGEFVVHKARASAFFGTHLITYLVRKNIDCLLVT
                     GTSTCGCVRGTVLDGYSYGYPVFVVEECVFDRARTSHLVNLFEMNAKYATVIQLAEAM
                     NYVNGLRKLEGRRAIAG"
     gene            complement(3058..4140)
                     /locus_tag="VF089_13490"
     CDS             complement(3058..4140)
                     /locus_tag="VF089_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175024.1"
                     /translation="MDQLKPASVLFIVYLVLIALVSFGTTEASAGSAPSPPREGESQN
                     ESQSESMNELYERAKKEGGKLVLYIALSPRSEEIVVPAFKKRFPEIQVNHIDATSDKL
                     VARAVAEARGGRVFGDIFGGTPGYLAQMRQQNLLAPLTIPEAAAYPANLKGGDWVATD
                     TQYFIAGWNTSLVKKGDEPKQFEDFADPKWKGKLIAEPRDFQLLMGLGKRKYKSDEKA
                     VALLKRIAANQVEFHKGHSQLAELLVAGQAAACITCYSHHFPPRIKKGAPVQALLSEG
                     VGEVGGSVTILRGAPNPAAALLWARWAVSEEGQRAYAQAGETPAHPNVEPGEKVRPAT
                     TYMLTADEVKEFPKYEKIWKEVFQLR"
     gene            complement(4200..5255)
                     /locus_tag="VF089_13495"
     CDS             complement(4200..5255)
                     /locus_tag="VF089_13495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175025.1"
                     /translation="MAAPVKEFEVHPYVQFMMDRYSPYKRWIESEGVPIIGGAFVKDV
                     RTEKVGYWKRKECNGAICTFSDQMVADAYIAEIDPGKSTKPQRQLYEEIITVAAGRGA
                     TSVWYEGTAKRVFEWERGSTFAIPLNAWHQHHNASGSEPCRYFALTSQPVAFELFRDP
                     EFIFNTNYMFKDRFDPGDPEFFSKEGQYFTEYYGGILHSNFIPDIRKINLVPREKRGK
                     GTRNMYIHMSGSAMLAHVSQFPVGRYKKAHRHGPGAHVFLLDSTGYTLMWNEGEKPQR
                     YDWHEATCISPPAGTWHQHYNTGKEPCKFVALHANTAVQREERGVEQIEFEDEDDSMR
                     KLYEEECAKNGVKVDMQ"
     gene            5509..>6078
                     /locus_tag="VF089_13500"
     CDS             5509..>6078
                     /locus_tag="VF089_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009332221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX6175026.1"
                     /translation="MSKAISFSLALLILLIVAAGAIEQSQIRPAQAAAQSADSIAVAF
                     KEYKLANGLRIILSEDPHAPTYSICVTYNVGSRDEKPGRTGFAHLFEHMMFQGSANVG
                     KGEHFILVLNNGGSANGTTNSDRTNYFQTLPANQLELGLFLEADRMRSLNINQANLDN
                     QRHTVQEERRQGVDNRPYGRTYEAVIGTAY"
BASE COUNT         1386 a         1659 c         1637 g         1396 t
ORIGIN      
        1 agctccttga tccgttgcgc gacaacttcg gcatcatcga catgaaagcc aaaatgatac
       61 atgccggccg cgtgacccgg ctctttagga tcgctggcat tcaagattgc caggtttact
      121 tcaccgtccg agagatgcac cgagccgttt ttggtgcggt acatttcttt tagctcaaaa
      181 actcttttat agaagtccgc gagtttttcc ggttgatgcg tcaagatcgc caggtgccgg
      241 agttttgcca taatcatgct ccttgttggg gggaagatct tcttgtctaa cactgagcgc
      301 tctcgccggt caaaccaaac cgtcgaagtt tcgtatcctc gggcaacgac aagccgactg
      361 cggcagtctg gctaaattgc ttaattgact tggataattc gatttgctac tagactaaaa
      421 gtcatttctt attggaggag aggaagaata gatgacctat gtgattgctg aaccttgtat
      481 cgacaccaaa gatactgcat gtgttgacgt ttgtccggtg gactgcatcc atcccaccaa
      541 aaacgaaact gaggagtttg aaaaggaaaa caagctctac atcgatcctg aggagtgtat
      601 tgattgtggc gcatgcgagc cggtctgtcc tgtggaggca attttcgagg aggcggcggt
      661 gccggataaa tggaagcact tcatcaagat agacgccgac tggtttaagg ccaaaaaagg
      721 ctagctccac gtctcatatt cttcggaaac ctctcaaggg ctgagcaatc ctcagccctt
      781 ttttaatgcg cgcaattctt actgccgctc ggctgtactt gtaatcctac tagctcgccg
      841 cctttagcgt tcgctagcgc ggtgcttttc cctccaaccg cacaccgata atacgcgaca
      901 tcagtaaccc ggccgaaaat agttcctgct gcaccgcctt gactcccttc ctttatctgt
      961 tgtacgcagg cagaagctta gagcgaaagg atacggcgca tggttctggt tctcaaaaac
     1021 gaacagatgg aagatctcct gccgatggcc gaagagatcg atgccatcga ggaggctttc
     1081 cgtgagctgg gcgcgggcca agcaatgaat gcgccgcgtg cgcggctgag aacgccttgg
     1141 aaagaggagg gcgggcagta ctattttaac aatatcatgg gcctggtgcc gggagttaaa
     1201 tccatggcgc tgcgcatcga ttccagcttc tcgaaagaag tccagattga cggcgccaaa
     1261 cggcgcattt atcccggtga ttttatcggg ctggtgatgt tgttcgacat ggacagctgc
     1321 gaattgctgg cgatcatgga cgatcatcat atctccgcga tgcgcgtggc tgccaccagc
     1381 gccgtcgcca gcaagtacct ggcgcgccaa gactcgaaag tcttggggct gctgggttcc
     1441 ggtgaacagg cgaaaaccca ggtcaccgcg catctgtcga ttcgtcctct tagcaaagtc
     1501 aaagtataca gcccgaccaa agaaaatcgt gaaaagttcg cctgcgatat gagcgcaacc
     1561 accggcatcg aggttgtgcc ggtggccagc gccgaagaag cggtgcgcgg cagcgatatc
     1621 gtcagcgctg caacgaacac cgtcgatccc gtggttcagg gccgctggct ggaagagggc
     1681 atgcatatca ccagcctggt cggcggcgac ggctttctac cgcgcaaaga actggacgat
     1741 gaggtgatta ccagagccgc cctgatcgtt gtcggttaca agccgcaaat attcatcgac
     1801 aagcaggcag agttccacga gcgtctggag cgtggcatcg tcaaagccga agatttgcac
     1861 gagctcggtg atctgttaaa cggcaaatgc cgcgggcgga tcgacgacaa ggagatcaca
     1921 cttttcaaga acaacaccgg gatgggcatt caattcgccg ccacggcaag gaagatgtat
     1981 gaaaaagccc gtgaaaaagg tgtcggttcc gaattgccgt tggatctttt catgacccgg
     2041 cgtggagaca aaacctattc cccgtaattt taaacgaacg ctagaacctt gaccgaacaa
     2101 agcaaaccgg cgcgcccgtg cattggatac cgctggagcc ggtcaatgtg gaactaaacg
     2161 tgtccatgct atcggccaaa gctgcgcatc ccaatgccgg taagttattc atcgactacc
     2221 tggtttcgag agagggacag gaattgctga aaggcttcag acggatcggc ccacgcaaag
     2281 atgtgaagtc tgatccagcc aaacgtataa cacgaaattc agaggaggct taatcatgcg
     2341 agaatgggaa cagattattc cagaagaaga aagaaggatt tacgagaagg cgggctataa
     2401 aggtaaggac agtttcggcc gtaacccggc gctgttggtg atcgacgtca ttactggttt
     2461 taccggcacc aaaccgatgc ccgtcctgga tgccatcgac gagtttccca ctagctgcgg
     2521 gcaagtcgcc tgggatgcct tgccgaagat tcaacagctg ctccatgcgt gtcgcgatgc
     2581 cgacgtgccg gtgatttatt cgaccagcga tcctgatttt aaagctgcct tcggcaatgc
     2641 caccaagcgc ggcgtcgatg ccaccgattt cgaaaagctg gccgtggcat ttcccgaaat
     2701 gatcaaaccg aagaaaggcg agttcgtggt ccacaaagcc cgggccagcg cgttcttcgg
     2761 cactcacctg atcacctacc tggtgcgcaa gaacatcgat tgcctgctgg taactggaac
     2821 cagcacctgc ggctgcgtgc gcggcaccgt gctcgatggt tactcctacg gctatccggt
     2881 ctttgtggtc gaggagtgcg tcttcgatcg ggcccgcacc tcgcacctgg tcaatctctt
     2941 cgagatgaac gccaagtacg ccaccgtgat ccaactggcg gaggcgatga attacgtgaa
     3001 tggcctgagg aagttagaag gccgacgagc gatcgcaggc taaatgtatc aattagacta
     3061 gcgaagctgg aagacttcct tccagatctt ctcgtatttg ggaaattcct tcacctcgtc
     3121 ggcggttaac atgtaggtgg tcgccggcct caccttctcc cccggctcta cattcggatg
     3181 ggccggcgtt tcgccggcct gggcataggc gcgctgacct tcttcgctaa ccgcccagcg
     3241 cgcccacaga agcgcggcgg ccggattcgg cgcacctctg agaatggtga cgctgccgcc
     3301 cacctcgccg actccctccg acagcagtgc ttgaacgggc gcgccttttt ttattcgcgg
     3361 cggaaaatgg tgtgaataac aggtaatgca cgccgccgcc tggccggcga ccaatagctc
     3421 cgccagctgc gagtggccct tgtgaaactc cacctggttt gccgcgattc gtttgagcaa
     3481 agcaacggct ttctcatcgc tcttgtactt gcgcttgccc aagcccatga ggagctggaa
     3541 atcgcgcggc tcggcgatca atttgccttt ccacttcgga tcagcgaaat cttcaaattg
     3601 cttcggttca tcgcctttct tgacaagact cgtgttccaa ccggcgataa agtactgcgt
     3661 gtcggtggcc acccaatcgc ctcccttgag gtttgccgga taagccgcgg cttctggaat
     3721 agtgagcggt gcgagaaggt tctgctgcct catctgcgcc agatagcccg gggtgccgcc
     3781 gaatatatct ccaaagactc ttccgccgcg cgcttcggct acggctcgcg ctaccaattt
     3841 atccgatgtc gcatcgatat ggttgacttg aatttcggga aagcgcttct tgaaggccgg
     3901 cacgacgatc tcttccgatc gcggcgagag agcgatgtaa aggaccagct tgccgccctc
     3961 ttttttggcc ctttcataaa gctcattcat ggactcactt tgggattcat tttgggattc
     4021 accttcgcgc ggcggactcg gagcggaccc cgcggatgct tcagtcgtgc cgaagctaac
     4081 gagagcaatg agtaccaggt aaacaatgaa gagcacggac gccggtttta gttgatccat
     4141 gaaacctcca atggcggaac cgcaaactcg gtagcggcgg cttcttctag cagtccagac
     4201 tactgcatat ccactttgac cccgttctta gcgcattcct cttcgtaaag cttgcgcatc
     4261 gagtcatctt cgtcctcgaa ttcgatttgc tcgacgcccc tctcttctct ttgcaccgcc
     4321 gtgttggcat gaagcgcgac aaacttacac ggctccttgc cggtattgta gtgctggtgc
     4381 caagtcccgg ccggcggtga gatgcaggtc gcttcgtgcc agtcgtacct ttggggtttt
     4441 tcaccctcgt tccacatcaa agtataaccg gtggagtcga gcagaaacac gtgagcgccg
     4501 ggaccgtggc gatgcgcctt cttgtaacgc cccacaggaa actgtgaaac gtgagccaac
     4561 atagcgctgc cggacatatg aatatacatg ttgcgcgtgc ccttgccgcg cttctcgcgt
     4621 ggcacgagat tgattttccg gatatcgggg atgaagttgg aatgaagaat gccgccgtaa
     4681 tactcggtga aatactgccc ttctttggag aaaaattcgg gatcgccggg atcgaaacga
     4741 tctttgaaca tataattcgt gttgaagata aactcgggat ctcggaaaag ctcgaacgcc
     4801 acgggctggc tagttagcgc aaaatagcgg caaggttcgg agccgctggc gttatgatgc
     4861 tggtgccatg cattaagtgg aatcgcgaag gtgctgcctc tttcccattc gaaaactcgt
     4921 ttggcggtgc cttcatacca aacactggtt gcgcctcggc cagcagcgac agtgatgatt
     4981 tcttcgtaga gctggcgttg aggtttggtg ctttttccgg gatcgatctc ggcgatgtac
     5041 gcgtcagcaa ccatctgatc ggaaaacgtg cagatagccc cgttgcactc tttacgtttc
     5101 caatatccaa ctttctcggt gcgcacgtcc ttgacaaacg cgccaccgat gatcggcacg
     5161 ccttcggatt ctatccatct tttataggga ctatacctgt ccatcatgaa ctggacataa
     5221 ggatggactt caaactcctt caccggcgcc gccatagaaa ctcacctcga cgaacttgat
     5281 ggctgacgtt accgccggga aataagcgct gtcaacccga ctcgaatgat cgcgacatcg
     5341 gttttcggat tatctcgatg gaggagtagc cggccgatgc agcgaccgga gcagtacgct
     5401 tgcgctcccg aacagtccca atatactgag tggaatgact gttttgcatc gccggtgaaa
     5461 agatgtggcg gtggctagtc taatcaactt tcaactctgg agcatgactt gtctaaagcc
     5521 atttcttttt cgcttgcgct gctcatcctg ctgattgtcg ctgcgggcgc gattgaacaa
     5581 tcgcaaataa ggccggccca ggccgccgct cagtctgccg atagcatcgc tgtagccttc
     5641 aaagaatata aacttgccaa cggcttgcgt atcattctct cggaagatcc gcatgcgcca
     5701 acctactcga tctgcgtcac ctacaatgtc ggctctcgtg acgagaagcc gggccgcacg
     5761 ggatttgctc acctctttga acatatgatg tttcagggct cggcaaatgt cggtaagggc
     5821 gaacacttta ttttggttct taacaacggc ggcagcgcca acggtaccac caattcggat
     5881 cgcaccaact actttcagac tttgccggca aaccagctcg agcttggcct atttctggaa
     5941 gccgatcgca tgcgctcttt gaacatcaat caggccaatc tggacaacca gcggcatacc
     6001 gtccaggaag agcggcgcca gggagtcgac aaccggccct atggtcgaac ctatgaagcg
     6061 gtcatcggca ccgcctat
//
LOCUS       DASUTG010000585         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1425882, whole genome shotgun sequence.
ACCESSION   DASUTG010000585 DASUTG010000000
VERSION     DASUTG010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425882"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF089_13505"
     CDS             <1..426
                     /locus_tag="VF089_13505"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX6175027.1"
                     /translation="APRTMQREPHYEDVVREVRDFLAERVCTAKKAGIALEQIIVDPG
                     IGFGKTLAHNLALLKNLQSFNSLGQPLLIGVSRKAFIGKILNAAGPEERLEGSVAAAV
                     AAALSGANILRVHDVSETVRALRVIDAIRFGLVPDSGVR"
     gene            423..1178
                     /gene="cdaA"
                     /locus_tag="VF089_13510"
     CDS             423..1178
                     /gene="cdaA"
                     /locus_tag="VF089_13510"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX6175028.1"
                     /translation="MTEVLGQLRWQDGLDIGIIWYLIYRLLQMLRGSRAMQMMIGLAV
                     VLMAYVLSRGLGLFTLNWILDNFLASIILVIIVIFQSDIRRALTQVGTAPIFGAEKMV
                     QRREDIIEEVTDAVRNLASRRVGALIVLEREVGLNEYIEIGTRLDARVSRELIESVFL
                     PGSPIHDGALVIQKRRVTAVRCLLPLSANPNLRTTMGTRHRAAIGVTEETDAVAIVVS
                     EQEGTIALVVGGNLTERLEASRLRNALEGLVKL"
     gene            1175..2104
                     /locus_tag="VF089_13515"
     CDS             1175..2104
                     /locus_tag="VF089_13515"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX6175029.1"
                     /translation="MKQIWRQIGSTASSNVGLAVLSFVIALGLWIAGHRDIERAIEVP
                     VEFRNIPSDLMVLDNRVDYVVLRLSGPRTLVSTLDADDLKLPLDLHGSKSGSLSFPLG
                     PSSFNIPRGITVARITPPIIHLRLDPVIKRELPVSIRLSGKPLAGYKIEETAVQPESI
                     SVRGPADEIRRMGFVETLPIDVEGSRSSIKRTVRLSTDGKPLSFMPEQVTVAITIVEE
                     DSVKDFKNVEIGARGVSGDYVITPKVAQVRLFGPRRILDKLQLGADHVYLDLDGLAPG
                     EHSVELEFNLPAEIKVAEQKPARFKVRIAKPHP"
     gene            2124..3575
                     /gene="glmM"
                     /locus_tag="VF089_13520"
     CDS             2124..3575
                     /gene="glmM"
                     /locus_tag="VF089_13520"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6175030.1"
                     /translation="MANVLKQKRRLFGTDGIRGIANQEPMTSEMALKLGRAIAKVLHD
                     PIAQPAQGSGRSKSPSLVKTLGGDTAHRYKILIGKDTRLSGYMLETALASGICSMGAD
                     VQLVGPLPTPAVAFLTRTMRADAGVVISASHNPYQDNGIKFFSFDGFKLPDEVEARME
                     EMIFSGEADRERPTASQIGKAVRISDAVGRYNVFVKNSFPRHLTLEGLRIVVDCGHGA
                     AYRVVPEMLSELGAQVIPIGVQPDGENINYRCGALYPETARDVLLHEKADFAVSLDGD
                     ADRAIFIDEAGEILDGDQVLAICAKDMQEKGTLKGHTVVATVMSNLGLDLALKSFGVN
                     LERTEVGDRYVVERMLERGYNLGGEQSGHILFLDHNTTGDGAITCLQVLALMVEKGKR
                     LGELKRIFTRMPQVLLNIRVTERKEFQAMPKVSRAIAAVEQSLNGRGRSLVRYSGTEM
                     LARIMLEGEDEDKICAMAQEIADEIRREIGVKD"
     gene            3572..4294
                     /locus_tag="VF089_13525"
     CDS             3572..4294
                     /locus_tag="VF089_13525"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX6175031.1"
                     /translation="MIRLGVNVDHVATVRQARRVDVPDPVEAALLAEAAGADGITVHL
                     REDRRHIQERDVQLLRERLQTKLNLEMALTPAMVAFAENIRPDDACFVPEKREELTTE
                     GGLDVVSHRQKISEAARRLQSLGVRVTLFVDPERAQIEASREACAHGVEIHTGAYCNA
                     LRIEHEKQLRAIVEASKFAQSLGLEVHGGHGLNYDNVLAIAQISEMTELNIGHSIIAR
                     AIIVGIEQAVREMKHLLTKSKE"
BASE COUNT          970 a         1151 c         1315 g          981 t
ORIGIN      
        1 gcccctcgaa ctatgcaacg ggagccgcac tacgaagatg tggtccgcga ggtgcgagac
       61 tttctggcgg agcgtgtttg caccgctaaa aaggcgggaa ttgccctgga acagattatt
      121 gtcgatccag gcatcggctt cggcaagacc ttggcgcaca atctcgcgct gctcaagaat
      181 ctacagtcat ttaactcgct tggccaaccg ctgttgatcg gcgtctcacg gaaagcattc
      241 atcggcaaga ttttaaatgc ggcaggtcca gaggagcggc ttgaggggag cgtggctgcg
      301 gcagtcgcgg cggcattgag cggcgccaac attctacggg tgcacgatgt gagcgaaacg
      361 gtgcgcgctc ttcgcgtgat cgatgcgatc cgctttgggc ttgtgcctga ttcaggtgtg
      421 cgatgacaga agtattgggt caactgcgct ggcaggatgg cttagacatc ggcataattt
      481 ggtatctgat ttatcgcttg ttgcagatgc ttcgcggcag ccgtgcgatg cagatgatga
      541 tcggcctggc ggtcgtcctg atggcgtacg ttctatcccg cggccttggt ttgttcacgc
      601 tcaactggat cttagataat ttcttagcgt cgatcattct cgttataatc gttatctttc
      661 agagcgatat tcgccgcgcc ttgacgcagg tgggaacggc acccatattc ggcgccgaga
      721 agatggtgca gcggcgcgag gacattatcg aagaagttac ggacgccgtt cgcaatctcg
      781 cgtccaggcg agtcggagcc ctcatcgtgc tggagcgtga agtcggtctg aacgaataca
      841 tcgagatcgg cacccgattg gatgcccgag tcagccgcga gctcatcgaa agcgttttct
      901 tgccaggctc gccaattcat gacggcgcgc tggtgattca aaaacgacgc gtaaccgcag
      961 tgcgctgtct cctgccattg agcgcaaacc ctaatcttcg cacgaccatg ggtacccgcc
     1021 atcgcgccgc gattggagtg acggaagaaa ccgatgccgt agccattgtg gtgtctgaac
     1081 aagaggggac gatcgctttg gtggtcggcg gcaatcttac cgagagactc gaggcatcgc
     1141 gattgcgcaa cgcgctcgag ggtctcgtga aattatgaag cagatctggc ggcaaatcgg
     1201 ttcaaccgcc tcaagcaatg tcggtctggc agtgctctcg ttcgtcatag ctctagggtt
     1261 gtggattgcc ggccatcgcg atatcgaacg agccatagag gttcccgttg agtttcgcaa
     1321 catcccatcc gatctaatgg tgctcgataa ccgcgtcgat tacgtcgttc tacgactcag
     1381 tggcccccga accctagtct ccactcttga tgcggacgat ttgaagctgc ccttggacct
     1441 tcatggaagc aagtcgggct cgttgagctt tcccttgggg ccaagctcgt ttaacattcc
     1501 tcgcgggatc accgtggcgc ggatcacgcc gccgatcatt cacttgcggc tcgacccggt
     1561 gatcaaacgg gaactgccgg tgagcatcag gctttccggc aagcctttgg ccggctacaa
     1621 aattgaggaa acggcggtcc agcccgagtc gatctccgtt cgggggcccg ctgacgaaat
     1681 tcggcgcatg ggttttgtcg agactcttcc aattgatgtc gagggaagtc gcagctcaat
     1741 caagcgcacg gtccggcttt caaccgacgg aaagccattg tcatttatgc cggagcaagt
     1801 aacggtcgcg atcaccatcg tcgaagagga ttcggtcaag gacttcaaaa acgtggagat
     1861 tggcgcccgc ggagttagcg gcgattacgt gataacaccg aaggttgcgc aggtgcggct
     1921 tttcggtccg cgcagaattc ttgataaatt gcagctcggc gcagatcatg tctatctgga
     1981 tcttgatggc ttggcgccgg gcgagcactc tgttgaatta gagttcaacc tgccggctga
     2041 aatcaaagta gcggagcaaa agccggcgcg ctttaaagtt agaattgcta aacctcatcc
     2101 ttaggtccgc aaggagtggc atcatggcta acgtcttaaa acaaaagcga cgcctgttcg
     2161 gcactgatgg tatccgcggt attgccaatc aggagccgat gacctcggag atggctttaa
     2221 agttagggcg agccatcgcc aaggtattgc atgatcctat cgctcagccc gcccagggct
     2281 caggccgctc gaaatcgcca tcgttagtga aaactctcgg cggcgatacc gcgcatcggt
     2341 acaagattct gatcggcaaa gatactcgcc tttccggcta tatgctggaa accgcgctgg
     2401 cttccgggat ttgctcaatg ggggcggacg ttcaactggt tggtccgtta ccgactccgg
     2461 cggtggcgtt tctcacccgc accatgcgcg cggatgcggg agtagtgatt tcggcttccc
     2521 acaaccccta tcaggataac ggaataaagt ttttctcctt cgacggtttc aagctgcccg
     2581 acgaagtcga agcgcgcatg gaagagatga tcttcagcgg agaagccgat cgcgagcggc
     2641 ccaccgcgtc gcaaatcggc aaggcggttc gcatttccga cgcggtaggg cgttataatg
     2701 tcttcgttaa gaatagcttt ccgcgccatt taaccttgga ggggctgcgc atcgtggtcg
     2761 attgcggtca cggtgcggcc tatcgagttg ttcccgagat gttatcagag ctcggcgctc
     2821 aggtgattcc gataggcgtg cagccggacg gcgaaaatat caattatcgc tgcggcgctc
     2881 tctaccctga aactgcgcgg gacgtattgc tccacgagaa agccgatttt gccgtttctc
     2941 tcgatggcga tgccgaccgt gctattttta tcgatgaagc aggcgagatt cttgacggcg
     3001 atcaagtgtt ggccatttgc gctaaagaca tgcaggagaa gggcaccctc aaaggacaca
     3061 cggtcgtggc cacggtgatg agcaatctcg ggcttgatct tgcccttaaa tcgttcggcg
     3121 tgaatctcga acgaaccgaa gtaggggatc gctacgtggt cgagcgcatg ctggaacgag
     3181 gctacaatct tggcggtgaa cagtcgggtc acattctgtt tttagaccac aataccaccg
     3241 gtgacggcgc cattacttgc ttacaggtgc tcgccctcat ggttgagaaa ggtaagcgcc
     3301 tgggtgagtt gaagcgtatc tttacgcgta tgccgcaagt gctattaaac attcgggtga
     3361 cggaaagaaa agagtttcag gcaatgccta aagtgagccg cgcgatcgcc gcagtggagc
     3421 aatcgttgaa cgggcgtggg cgcagcctgg tgcgctattc cggcaccgag atgctggccc
     3481 ggatcatgtt ggaaggtgag gatgaagata agatttgcgc catggcgcag gagatcgccg
     3541 atgagatacg gcgggaaata ggagtgaagg attgattcgc ctcggagtga atgtggacca
     3601 tgtcgcgacc gtgcgccagg cgcggcgggt agacgtgccg gatcccgtcg aagccgcttt
     3661 gttggccgaa gcggccggcg ccgacggcat aaccgtgcac ctgcgcgaag accggcgcca
     3721 tatccaagag cgagacgtac agcttctgcg cgaacgcctg caaacgaaac tgaatctcga
     3781 aatggcgcta acccccgcca tggtcgcttt tgctgagaac atccgtcccg atgacgcttg
     3841 tttcgtgccg gagaagcggg aggagctcac cacggaaggc ggtttagacg tcgtttcgca
     3901 tcgacaaaag attagcgagg cagcgcgtcg attgcagagc ctaggcgtgc gcgtgacttt
     3961 gtttgtcgac cctgagaggg cgcagatcga agcgagccgt gaggcatgcg cgcatggggt
     4021 tgaaattcat accggcgctt attgcaatgc gctgcgtatc gagcacgaaa agcagctgcg
     4081 agcaatcgtc gaagcctcga aattcgcgca gagtctcgga ctcgaggttc acggcggcca
     4141 cggactcaac tacgataatg ttttggcaat cgcgcagatc tctgagatga ccgagctcaa
     4201 catcggtcac agtattatcg ctcgagcgat tatcgtcggc atcgagcaag cggtccgcga
     4261 gatgaagcat ctgcttacca agtctaaaga ataaacaggt tcaaaccgct caaatccttt
     4321 agatccggcg cctttaacgt ttgagtattt tgaaccgatt tgaacgatct aattatgatt
     4381 gtcgttacct ccgagcagat gcgcgagatg gatcgat
//
LOCUS       DASUTG010000586         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1721882, whole genome shotgun sequence.
ACCESSION   DASUTG010000586 DASUTG010000000
VERSION     DASUTG010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721882"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF089_13530"
     CDS             <1..203
                     /locus_tag="VF089_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175032.1"
                     /translation="IDTEMTARTYDSSVKTFSENLALPEEGLRLLIDDAKRMAKVNRE
                     VSLSDVADLSVLKSVQRELGSR"
     gene            268..1317
                     /locus_tag="VF089_13535"
     CDS             268..1317
                     /locus_tag="VF089_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175033.1"
                     /translation="MTISRLGSYRQWLPQRFRGLLLAALVVVFLWAQTLSALAGQAKP
                     LVKVVIGYGSADGGVAVLGFAKEAKLFEKHGLDVVLVGMGTGSVSLRALIAKDLQISS
                     LSGSGLVQAAMQGADTVLIAALVNGFVFKIFSAPEISNPTQLKGKKLGVSRYGATSDF
                     AVRLALKKWGLNPERDVNILQIGTSQDTVRALQAKMLDAGVTTGTSSLIARKAGFREL
                     ADLADLGLDYPTSAIGTTKSYLQKNEATVKGFMLAYIEAIHDFKRNKEAAIGVLRKYT
                     RTDDREVLEHNYNEIANKYLPLPIPTLEGIRTILTELSSTTPAAKNADPEHFVSYKIA
                     REIEASGFVKRLYQK"
     gene            1378..2139
                     /locus_tag="VF089_13540"
     CDS             1378..2139
                     /locus_tag="VF089_13540"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175034.1"
                     /translation="MTDYVKKLLPRFRYGVIQPRAHEEVQRARSYQLYRLLPLDFIEI
                     STGLGLENYTREGVEKAIKNYWTCVDRLAKEKVDHIIFSGAPISAMLTRPRVLELLRE
                     TKERTGISADAPLEALIAAMKHLGLTKVAIGSRWADPVNDAIKRYLEAGGINVLGITK
                     RNQWASDSFAMTLEQGLEMALDVGREAARTWPEAEAISVPGGAAMSLHVIPAIEEEYG
                     KPAFTNMSAEVWNDLVRPGIIPPVQGWGRLLANVK"
     gene            2317..3552
                     /locus_tag="VF089_13545"
     CDS             2317..3552
                     /locus_tag="VF089_13545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175035.1"
                     /translation="MHRAHLIAVLTVITFGWFAGCSMPVSEQSVTAYERARLIVGDGR
                     VIENATLVVQDAKLAQVGRAADVRVPAGARRVDLAGKTVMPMIVDTHVHLNDNREAVI
                     RDLRMRAYYGVSAVLSLGTDGYEVLDLHSQTIAGAARYRSAGRGITMPEPGRTKVPYW
                     VTSAAEGRKAVEELAAHKVDIVKIWVDDRDGKYKKLTPEIYGAIIDEAHKRRLRVTAH
                     IFELADAKGLIRAGVDSFAHGIRDRDIDDEVLTMFKQRPNLILTPNLPDRGVKADISW
                     LRASLPQDEFKKLEGANTDRPKAQALYGIQARNLAKLNAAGVRIVLGTDGNRPWGPHE
                     EMLDMVMAGMTPMQVIVAATRNSAEFLQLADAGTLEAGKSADFIVLDANPLDDITNTR
                     RISAVILRGTAVDRSQPVR"
     gene            complement(3831..4943)
                     /locus_tag="VF089_13550"
     CDS             complement(3831..4943)
                     /locus_tag="VF089_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HEX6175036.1"
                     /translation="MAQDGELRLAILSYGDPDARPNTTVENSRFADLFRAFAARRVHV
                     EPAIYHDDFCDEVREQLMPIDGVLVWVNPIEGGQDRSELDSMLRDVAAAGVFVSAHPD
                     VILKLGTKEVLYRTRNLEWGCDTYLYASLDQLVQTLPLRLADGKARVLKQRRGNGGNG
                     VWKVQLRIDELANRTGTASTALPQPQTVIRVRHALRGCTEEEITLSEFYRRCEPYFSA
                     NGLMIDQEYQERLAEGMIRCYLVHDKVVGFGHQAINALFPALPGAPPSEAPPPGPRLY
                     HPPTMAEFQILKRKLEQEWVPAAQRLLDIKTEDLPILWDCDFLLGPKRADGEDSYVLC
                     EINVSSVAPYPESAVPYVIDATLARLRAAAQKRRRA"
     gene            complement(5118..>5319)
                     /locus_tag="VF089_13555"
     CDS             complement(5118..>5319)
                     /locus_tag="VF089_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175037.1"
                     /translation="MLRYCADFFVLTRCVSSKPHHTGWTAGSHHLSASLGDHEGTVKK
                     VAIPVGDFHMGNPGFSIKNIID"
BASE COUNT         1165 a         1464 c         1551 g         1139 t
ORIGIN      
        1 agatcgatac ggagatgact gcccggacct atgattcaag tgtaaagaca tttagcgaga
       61 atctcgctct tccggaagag gggcttcgac ttctgattga cgatgccaag cggatggcaa
      121 aggtcaaccg tgaagtttct ctgagcgatg ttgcggatct ttcggttctt aaatcagtgc
      181 agcgggagct gggaagcagg tgatttgaag actgttcgtg agtgtgcgcg cctggctgtg
      241 cctgtgccct ttttcggtga gaaaattatg accatctctc gccttggatc gtatcgccag
      301 tggctgccgc agcgttttcg tggattactg ctcgcggcac ttgtcgttgt ttttctttgg
      361 gcccaaacgc tgagcgcatt ggcgggccag gccaagccgc tcgttaaagt cgtgatcggc
      421 tatggttctg cggatggcgg cgtggcggta ttggggtttg ccaaagaagc taaactattc
      481 gagaagcacg gtcttgatgt agttttggtc ggcatgggta ccgggtcggt gtcgttgcgg
      541 gcgctcatcg ccaaagatct gcaaatttcc tcgctctctg gttccggttt ggtgcaagcc
      601 gcaatgcagg gcgccgacac cgttctcata gccgctctgg tcaacggatt tgttttcaag
      661 attttcagcg cgccggaaat ttccaatccc acccaactca agggcaagaa attaggtgtc
      721 agccgctacg gggcgacatc cgacttcgcg gttcggctgg cgctcaaaaa atgggggctg
      781 aaccctgagc gcgacgtcaa tattctgcaa atcggtacat cgcaggatac agtccgcgcg
      841 ctgcaggcta aaatgctcga cgccggggtg acgaccggca cttcatcgct aatcgcgcgc
      901 aaagcgggct ttcgcgagct ggccgatctc gccgacctgg gtctggacta tccgacgtcg
      961 gccatcggca ctaccaaatc ttatctgcag aaaaacgaag cgaccgtgaa gggattcatg
     1021 ctcgcctaca tcgaagcgat ccatgacttc aaacgcaata aagaagccgc cattggggtg
     1081 ctaagaaaat atacccgcac tgacgatcgc gaggtgttgg agcataatta caacgagatc
     1141 gcgaacaaat atctgcccct gccgattccg acactcgaag ggattcgtac cattctgacg
     1201 gagctttcga gcaccacgcc ggcggcgaag aatgccgacc cggagcattt cgtctcgtat
     1261 aaaatcgcgc gcgaaatcga agcgagcggc ttcgtcaaac ggctgtacca gaagtaggag
     1321 gaaacctgac gagcggtatg taatccgctt gcatcaattt tcaaggagct cttggttatg
     1381 accgactatg tgaaaaaact tctgccgcgt tttcgttacg gagtaatcca gccgcgcgcc
     1441 catgaagaag tgcagcgcgc gcgaagctat cagctctatc gactcctgcc gctcgatttc
     1501 attgaaatct ccaccgggct cgggcttgag aattacacta gggaaggcgt tgagaaagcg
     1561 atcaagaatt actggacgtg tgtcgaccgc ctggccaaag agaaggtcga tcatattatt
     1621 ttcagcggcg cgccgatctc cgcaatgctg actcgcccaa gggtgctcga attgctgcgc
     1681 gaaacgaaag agcgaaccgg aatatccgcc gacgctccgc ttgaggcgct gattgccgcc
     1741 atgaaacatc ttggtttgac gaaggtcgcg atcggcagcc gctgggcgga cccggtcaat
     1801 gatgcaatca aacgctacct cgaggctggc ggcatcaacg tcctcggcat caccaagcgg
     1861 aaccagtggg catcggactc tttcgcgatg actttggaac aaggcttgga aatggccctc
     1921 gatgtcggcc gcgaggcggc aagaacctgg cccgaagccg aagcgatctc ggtgcccggc
     1981 ggcgcggcga tgtctctgca cgttatcccg gctatagagg aagaatatgg caaacctgct
     2041 ttcactaaca tgagcgctga agtatggaac gatctcgtgc gccccggcat catcccgccc
     2101 gtccaaggtt ggggaaggct cttggcgaat gtgaaatgat ttcttacatg aagtccttat
     2161 gttgcaccca gatcagaata tcttgacagg ttgcgaacgg cggaattgct gccgatttgg
     2221 ttgcgaatta cacgggctac gggggccgac cgggcttgct tccagtcgat ggtcgcttac
     2281 ttgaggatcg gagccttaat tgaatcagga ggtttcatgc atcgagcgca tttaatcgca
     2341 gtcctgacag tgatcacatt tggttggttt gctggatgtt cgatgcccgt gtccgaacag
     2401 tccgtcacgg catacgaacg cgcgcggttg atcgtcggtg atgggcgtgt gatcgagaac
     2461 gccaccttgg tggtgcagga cgcgaaactc gcgcaggtcg gccgggctgc cgacgttcgg
     2521 gtgccggcgg gcgccaggcg cgtggatctc gccggcaaga ccgtgatgcc gatgattgtc
     2581 gacactcacg tccacctcaa cgataatcgc gaggcggtca tccgcgactt gagaatgcgc
     2641 gcttactatg gcgtgagcgc cgtgctcagt ctcggcacgg atggctacga agtgctcgac
     2701 ctgcacagcc agacgattgc cggcgccgcg cgttatcgca gcgccgggcg gggcatcacg
     2761 atgcccgagc cgggacgaac aaaagtgcca tactgggtta cgagcgcggc ggaaggacgc
     2821 aaggcggtgg aggaactcgc ggcgcacaaa gtcgatatcg tcaaaatctg ggtcgatgat
     2881 cgagacggca agtacaagaa gctcacccct gaaatttatg gcgccattat cgacgaggcg
     2941 cacaagcgca ggctgcgggt cacggcacat atcttcgagc tcgcggatgc gaaggggctg
     3001 atccgtgccg gcgtcgattc gttcgcgcac ggtatccgcg accgggacat cgacgatgaa
     3061 gtgctgacca tgttcaagca gcgtccgaac ctgatcctca cgccgaatct gccggatcgc
     3121 ggcgtaaaag cggacataag ctggctgcgc gccagccttc cgcaagacga gttcaagaag
     3181 ttggaagggg ctaacacaga ccggcccaag gcgcaggccc tctacgggat tcaggcgcgc
     3241 aatctcgcca agctgaatgc ggcaggcgtg cgcatcgtcc tcggaacgga cggcaatcgc
     3301 ccgtggggcc cgcacgaaga gatgttggac atggtgatgg ctggaatgac gccgatgcag
     3361 gtcatcgttg cggcgactcg caactcggcc gaattcctgc agctcgccga tgcgggcacg
     3421 ctggaagctg gcaagagcgc cgacttcatc gtgcttgacg caaacccgct cgacgacatc
     3481 accaacacca gacgcatctc agccgtgatc ctgcgcggca cggccgtgga ccgcagccag
     3541 ccggtccgtt gagccacttc gatcgggttc gttgctggaa taggggcggt gagactttca
     3601 tcttggtctg tttgatccga actatttgcc cggacgggat cgcgttgttt cgtcagaatc
     3661 tgctgtcctc ggttctcgtg tgactggatc tgccgtgcct agtgtaattg ccaatcattt
     3721 tacttgctga tcgagactga aggacgattt cgatcagcat gcgcagctaa accggatctc
     3781 atccacagct tcgatcgccg atacgagcaa cctctcacga gaacaagtaa ttaggcacga
     3841 cggcgtttct gtgctgctgc tcgcagccgg gccagtgttg cgtcgatcac gtaaggcacc
     3901 gcagattcag gatagggcgc aacactgctg acgttgattt cgcaaagaac gtagctgtct
     3961 tcgccatcgg ctctcttcgg gcccagcagg aaatcgcaat cccagagaat cggcagatct
     4021 tcggttttga tatcgagtag ccgctgcgct gccggcaccc actcctgctc cagtttgcgt
     4081 ttcaggatct ggaattccgc catcgttggc ggatggtata aacgcggtcc gggtggcggc
     4141 gcttccgacg gaggcgcgcc ggggagcgcg ggaaatagcg cgttgatagc ttgatgcccg
     4201 aaaccgacta ccttgtcatg gaccaggtag cagcgaatca tgccttccgc tagacgctct
     4261 tgatactctt gatcaatcat caggccattg gcggaaaagt aaggctcgca gcgacggtag
     4321 aattcgctga gtgtgatttc ctcttcggtg caaccacgca aggcatgtcg tacacgaatg
     4381 acggtttgag gctgtggcaa ggcagtggaa gccgtgcctg ttcgattggc taactcgtct
     4441 atcctcagtt ggactttcca tacgccgttt ccaccatttc cccgtcgctg cttgaggacc
     4501 ctggccttgc cgtcggcaag ccgcaaaggc aacgtttgaa ccagctgatc caaactggcg
     4561 tagaggtatg tgtcgcagcc ccattcgaga tttcgtgtgc gataaaggac ttcctttgtc
     4621 ccgagcttca agatcacgtc gggatgagcg ctgacaaaaa caccagcagc cgcaacatca
     4681 cgaagcatag aatccagctc cgacctgtcc tgtccgcctt caatgggatt gacccacacc
     4741 agaacgccgt caatcggcat gagctgctct cgaacttcat cgcaaaaatc atcgtgatag
     4801 atcgccggct cgacgtgaac ccgcctggcg gcaaacgccc tgaacaaatc tgcgaaccgg
     4861 ctattctcca cggttgtatt cggcctcgca tcgggatcgc cataggagag aatggctagt
     4921 cgcagctcac catcttgtgc catggacacg ttcctttccg gaccgagtct cgtcccatac
     4981 tatcaagtga accgccgtag caaacgaaat cagtgctcga cagactagcg ggtcaccttg
     5041 agcgccgaac atggcgctag aacgtgagtc tcgcgagcgt cttgaggccg tggtgagaag
     5101 ttgaagtctt ttctatgtca atcgattatg ttcttgatac tgaatccagg attgcccata
     5161 tggaaatccc cgacgggtat cgcgaccttc ttgaccgtcc cttcgtggtc tcctaacgac
     5221 gctgataagt gatggctccc tgcagtccag cccgtgtggt gcggcttaga ggagacgcac
     5281 ctggtgagca cgaaaaagtc ggcacaatat cgcaacatg
//
LOCUS       DASUTG010000587         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1721923, whole genome shotgun sequence.
ACCESSION   DASUTG010000587 DASUTG010000000
VERSION     DASUTG010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721923"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF089_13560"
     CDS             <1..497
                     /locus_tag="VF089_13560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6175038.1"
                     /translation="FPDFTAVIIGAVTSDQTKFARHLKGIIAAAGLSQRIHFLGERPA
                     AEIPLWFRRTSIVVAPQRWEGFGLVPLEAMASGCAVVATRVGAAYHVIVDEHTGYLVS
                     PEDLDGLIGRLEILMRDPQLTIRMGQAGRAHAVNHFSIEREAAELERVYESVWHGVGT
                     SAGG"
     gene            715..1368
                     /locus_tag="VF089_13565"
     CDS             715..1368
                     /locus_tag="VF089_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX6175039.1"
                     /translation="MVNKPAGLLTIATDSEKTRTAYFILTDYVRKGHRRSRNRIFIVH
                     RLDRETSGILIFTKTAEAKLYLQQHWETTEKKYLAVVHGRMEKGMETISTYLVENKAH
                     MVYTTSDRSKGRFGRTAYRVLKQARDFALLEVDLLTGRKHQIRVHLADIGHPVVGDTK
                     YGKENRGYRHLALHAHSLSVTHPFTGERLTFETAAPAYFSHLMGNLESGLGRIIHDR"
     gene            complement(1598..>2865)
                     /locus_tag="VF089_13570"
     CDS             complement(1598..>2865)
                     /locus_tag="VF089_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6175040.1"
                     /translation="GPVYICYDGDVQEKQLEREVTFPSLDRYPAPLPLQAPEEGFDKT
                     IRWLLEAKKPVIIADWVGRKEAGFQALGELAEILGAPVLDQFGRFNFPVQHPLNLTGQ
                     SDKVIPQADLILALDVPDLEGATTRRAQEKGNRRPTPLMQAGAKIINVGLDDLLVRAW
                     ATDFNKLREADLMITADTSAFLPELVRRLKSEKKLSDLKEDIGRRRADWGKIYEDKQA
                     ALQKELKTKWDEKPISMTRIYAEMVEALKGEDWVLTNGSSQGKENLYLAAAKFNQILG
                     KYKGGGLGYGLPASLGAALALKGSGKFCVNIQPDGDLLFTVSGLWTAVHHQIPILSIV
                     CSNRSYFNDEEHQERVALQRNRPVENKTIGIRIEDPNVDFGAMARTYGCWGAGPITEP
                     KDLIKTLREAVKVVKEGKPALVDVVCQMR"
BASE COUNT          620 a          816 c          776 g          653 t
ORIGIN      
        1 agtttcccga cttcaccgcc gtgatcatcg gcgctgtgac atcggaccaa actaaatttg
       61 caagacacct caagggcatc atcgcggctg ctggtctgtc gcagcgcatc cactttctcg
      121 gtgaaaggcc cgcggctgaa atccctctat ggtttaggcg cactagcatc gtcgtcgcgc
      181 cgcagcgttg ggagggtttc ggactcgtgc cgctcgaagc aatggcgagc ggctgtgcgg
      241 tagtggcaac tcgcgtcggc gcggcttacc acgttatcgt cgacgagcat accggctatc
      301 tcgtatcgcc tgaagatctc gacggattga tcggtcgcct tgaaattctg atgcgcgacc
      361 cgcaactgac gatcagaatg ggccaagcgg gtcgggctca tgcggtcaat cacttttcga
      421 tcgaacgcga ggcggccgag ttggaacgcg tttacgaaag cgtttggcac ggcgtaggca
      481 ccagcgcggg tggttagatt tccgcattta gctttaataa agagtcaagt tcatcttctt
      541 acataactac ggagttgcgc gcgcgttact aggttcaacc agccagttag ccggtttttg
      601 cgcgcgacca gataggctta ttcaaactca atggttcctg tgatctccgc tcgtcctgtc
      661 acacgaaatc ttcagtgcgg cctcgccctt ctttacgaag acagggacat tctggtggtg
      721 aacaaaccgg ccgggttgtt aacgatagcg actgattcgg agaaaacccg gacggcttat
      781 ttcatcctga ccgactacgt gcgcaagggg cacaggagat cgcgcaatcg gattttcatc
      841 gtgcaccggc tggatcgtga aacatcgggc atactcatct ttaccaagac ggccgaagcg
      901 aagttgtatc tgcagcagca ttgggagacc actgagaaga aatatttggc ggtggttcac
      961 ggccggatgg aaaaaggtat ggagactatt tctacctacc tcgtagaaaa caaagcgcac
     1021 atggtttaca cgacgtcgga cagaagcaaa ggcagattcg gccgtaccgc ctatcgagtg
     1081 ctcaaacaag ccagagactt tgcccttttg gaagtcgatc tattgaccgg tagaaaacat
     1141 cagatacgcg tgcatctcgc cgatattggc catcctgttg tgggagacac gaagtacgga
     1201 aaagagaatc ggggctaccg ccatctagcg cttcacgctc attcgctttc agttacacac
     1261 ccgttcacag gtgagcgact cacttttgaa accgcggcgc cggcttattt tagtcaccta
     1321 atgggtaact tagaatcagg attaggccga attattcatg atcggtagga acagccatac
     1381 cacttgtagg ccgaagggtg aggccggaga ggcgaaggcg caaaacgtac aatctcactg
     1441 agttgtccgg tgcttggccg tacaatgaca acactagccg accgttcgct ccagtactga
     1501 ggcttgcatt cagcctcaag gcaaagacct atcgccttcg actccggagg actcactctt
     1561 cggcgatgac catctgagac agcataccgt ggcgaactca gcgcatctgg caaaccacgt
     1621 ccaccagcgc cggcttccct tccttcacca ccttgacggc ttcacgcagc gtcttgatga
     1681 gatctttggg ctcggtgatc ggtccggcgc cccagcaacc atacgtacgc gccatggcgc
     1741 cgaaatcgac gttgggatct tcgatgcgga tgccgatggt tttgttctcc acgggccgat
     1801 tgcgctgcag cgcaacgcgc tcctggtgtt cctcgtcgtt gaaataagaa cgattgctgc
     1861 aaacgatgct caggatcggg atctgatgat gcaccgcggt ccataagccg ctgacggtga
     1921 agagcagatc gccgtcaggc tggatattga cgcaaaattt tcccgagcct ttgagcgcga
     1981 gcgcggcacc caaggatgcc ggcaagccgt accccaaccc gccgcctttg tatttgccca
     2041 gaatctgatt gaacttggct gcggcgaggt acagattttc ttttccttgc gaactgccgt
     2101 tggtcaggac ccagtcctcg cctttgagcg cctcgaccat ctcggcgtaa attcgcgtca
     2161 tcgaaatcgg cttctcgtcc catttcgttt ttagttcttt ttgcaatgcg gcttgcttgt
     2221 cttcgtagat cttcccccaa tcggctcgac gccggccaat atcctctttc agatcgctca
     2281 gtttcttctc cgacttcagc cggcggacca actccggcaa gaacgccgag gtatccgccg
     2341 tgatcattaa atcggcttcg cgcagcttgt tgaaatccgt tgcccaggcg cgcacgagca
     2401 agtcatcgag accgacattg ataatcttgg cgccggcctg catcaacggg gtcggacgcc
     2461 ggttgccttt ttcctgcgcc cggcgggtcg tcgcgccttc aaggtcggga acatccaaag
     2521 ccaggatcag atcggcctgc ggaataacct tgtcgctctg ccccgttagg ttgagcggat
     2581 gctgcacggg aaaattgaat cgcccaaatt gatcgagcac cggcgcgcct agaatctcag
     2641 ccaattcacc gagcgcctga aagccggctt ccttcctgcc gacccagtcg gcgataatta
     2701 ccggcttctt ggcctcgagc agccagcgaa tggttttgtc gaaaccttcc tccggcgctt
     2761 gcagcggcaa aggtgcgggg tagcgatcca gcgacggaaa tgtcacttcg cgttcgagtt
     2821 gcttctcttg aacgtcgcca tcgtagcaga tgtaaaccgg cccct
//
LOCUS       DASUTG010000588         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1872033, whole genome shotgun sequence.
ACCESSION   DASUTG010000588 DASUTG010000000
VERSION     DASUTG010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872033"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VF089_13575"
     CDS             <1..773
                     /locus_tag="VF089_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175041.1"
                     /translation="EAHTATLGYRLTGEQGENWCTVYNEGIKGLIDSHPDRFVGLASV
                     PLQDPTRAARVLEHAVRDLKFKGGYIGTNVNGNYYGTPDFDPFWAKAQELDVMIVMHP
                     EDVAGADKMNPYGLKLICGNPADSALCFGYLTYSGVFDRFPNLKLCILHGGGFFPYHL
                     GRFDRGWQVRAGARAPQAKMPPSAYLKNIYYDNMIYRLDTIKYLIGLAGLEHIMVGTD
                     YPYDLGDWNAAKMVEELDISEADKLLMLEGNAKRLLKL"
     gene            complement(985..1248)
                     /locus_tag="VF089_13580"
     CDS             complement(985..1248)
                     /locus_tag="VF089_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175042.1"
                     /translation="MNKHRLNPDQFKCDWPEFKGELQKTWQDFSDDDLAEIEGDYDKF
                     VSMAQKRCRDKEEDVVRWANDWYSKREQHEAAARQSTISRNQM"
     gene            1520..2803
                     /locus_tag="VF089_13585"
     CDS             1520..2803
                     /locus_tag="VF089_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175043.1"
                     /translation="MRFQLNNFSFLVRALSSRNFRLFFFGQAASLIGTWMQQIAMRWL
                     VYRLTKSELMLGLVGFVSDIPLFLLVPFAGVLADRLKRHQILFVTQLLAAIQAAVLAL
                     LVITESVAVWHVILLGGILGIISAFDITARQSFIVDMIENKDDLPNAIALNSFIFNGA
                     LLIGPAVAGILIAAVGEGLCFAINSLSYLTVIGALLMMKIPKKKFSSSALGFKAAIIE
                     GATYTFASVPIRSILMFVAVVSFVAASYPLLMPVYAESTLQGGPRVFGYLMSATGVGA
                     LAGAIFLASRNNLKGLGEMIVIAAILYGVGYFAMALSNTLPISLIIALLLGFSLMMQM
                     ASSNTIVQTIVDDQKRGRVLSLFVMARRGIESLGSLLFGAIAAWIGTPGTMMIAGAIC
                     LLAGAAFATQVPSIREAGNSVGDNSDPAERRETNL"
     gene            complement(2856..3734)
                     /locus_tag="VF089_13590"
     CDS             complement(2856..3734)
                     /locus_tag="VF089_13590"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6175044.1"
                     /translation="MMDRSEVVLRVTPDSKLAEEWALVLITQGLSPSLHHDQRGMVLS
                     IPAEEAEMALAGLAAYENENPVPVPKEQNRSDDLPNSVAGLVVAGVLILSHAGTVRWK
                     EAVPWIERGSAEASRILDGELWRTVTALTLHADLVHLISNAVAAAIFVTALSSIVGAG
                     LAFTLLVISGACGNLVNSLVQGSSHIAVGASTAIFGAVGVLGSQGLIRRRRDPTRRRG
                     AWIPIAASLGLLAMLGTGTGRTDVLAHLFGFFCGAALGIPVGLFFPVRPGLLVQWLFG
                     SATAAVLIYCWTLALD"
     gene            complement(3853..5292)
                     /locus_tag="VF089_13595"
     CDS             complement(3853..5292)
                     /locus_tag="VF089_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6175045.1"
                     /translation="MDRNLIFIAGATGYIGGRLLTVLEGKGYRVRCLARHPEYLRSRI
                     GRDTEVVAGDVFAPETLDAALIGVDQAYYLVHSMGSAGRFEEQDRIAARNFAAAARRA
                     GVRRIIYLGGLGSGEALSSHLRSRQEVGKILRDSGVPVIEFRASIVIGSGSLSFEMIR
                     ALVERLPVMITPRWVSIIAQPIAVRDVLDYLASALELPRAESRIFEIGGNDRVSYRDI
                     MAEYARQRGLRRIMISVPVITPRLSSLWLALITPVYARTGRKLVDSIRNPTIVRDPAA
                     LAAFDIRPLGMAAAIAAALRNEDLEFAATRWSDSLSAAGAQPTWAGVRFGNRLVDSRV
                     TDVKVPSERVFAVICRLGGATGWYFANWLWQLRGWFDLLVGGVGLRRGRRHPEELRVG
                     DALDFWRVEAVDPNRRLRLLAEMKLPGRAWLEYVLEPHGSGTRITQNAIFDPVGLVGL
                     AYWYAVYPLHRILFGGMLSAIARKAEQIN"
     gene            complement(5493..>6234)
                     /locus_tag="VF089_13600"
     CDS             complement(5493..>6234)
                     /locus_tag="VF089_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6175046.1"
                     /translation="ELDFGFMASNWIGRAKEGTAPFTREIDLRMVAPANVGPMFFVKR
                     ADSPIQSIAEFKGKRIAIGTKGSGMEQHVQTIFAVLGIGFDSFTPLNMSFAQGADELI
                     AGNIDAQFQPPIPNRVMTDLSLRADVRVIPYAPGQLPRVVGEVPFYRSVIIEKDAFRG
                     VVEDIAQLGVVNVLVTHARTSESIVHNMARAIVDNLDNLPNMNRLFRGLKNLFEPLRV
                     EGRSAFEFGDVPLHPGAERAYREADWLK"
BASE COUNT         1419 a         1784 c         1652 g         1379 t
ORIGIN      
        1 tggaagccca tacagcgacc ctgggttatc gcctcaccgg cgagcaaggc gaaaactggt
       61 gcaccgtata caacgaggga atcaaaggtt tgatcgatag tcacccggat cggtttgtcg
      121 gcctggcgtc ggtgccactg caagacccga cccgcgccgc cagagtcctg gagcacgcgg
      181 tgcgcgattt gaaatttaag ggcggctaca tcggcacgaa cgttaacggc aattattacg
      241 gtacgccgga tttcgacccg ttctgggcca aagcgcaaga gctcgacgtc atgatcgtga
      301 tgcatccaga ggacgtggct ggtgccgata aaatgaatcc ctatggtttg aaactgattt
      361 gcggcaatcc ggccgatagc gcgctgtgct tcggctacct gacatacagc ggcgtattcg
      421 atcgctttcc caatctcaag ctctgcatac tccacggcgg cggatttttc ccgtatcatc
      481 tagggcgctt cgaccgcggc tggcaagttc gcgccggcgc gcgcgcgccg caggcgaaaa
      541 tgccaccgag cgcatatcta aagaatatct actacgacaa catgatttat cggctggata
      601 ccatcaaata tctgatcggc ttggccggcc tggagcatat tatggtcggc acggattacc
      661 cgtatgatct cggcgattgg aacgctgcaa aaatggtcga ggaactcgat atctccgaag
      721 ccgacaagct actgatgctc gaaggaaacg ccaagcgctt gctgaagttg taactgcatc
      781 tgactaacga acgaatctag ttcagcagta cagccgcggg ctctgacctc gactcaaagc
      841 aacgtgtcca attaagatac gtccctgacc ctgactgcag atgagaagtt gcttcggccg
      901 gacgctaaac ggcgaacgat gtgtgcacac catagaggcg ctgcacttta tcgcaacacg
      961 ctcagccgat cccagctaaa cagattacat ttgatttcgt gatatcgtgg attgtctggc
     1021 ggcggcttcg tgttgttcac gtttggagta ccaatcgttg gcccagcgca cgacgtcctc
     1081 ttccttgtca cggcaccttt tctgcgccat cgaaacgaac ttgtcgtaat ccccttcgat
     1141 ttctgccaga tcgtcgtccg agaagtcttg ccaggtcttt tgcagttcgc ctttgaattc
     1201 aggccagtca catttgaatt gatccggatt gagtcgatgc ttgttcataa aacctccctt
     1261 acgtcgttca ggttgctttt gcatacgagg tgccaaaacg gctttgctat atcgcgagaa
     1321 aaaatttcat aggttttact ctattacagt cgtcgtgttt catgtttgaa acaaaagtcc
     1381 cgcatcgggg acaacctacg gacgcgagac tagaataaga atcggcgctg ggctttttga
     1441 gcacaagcag tttccgctga acaatgagcc catgcgccaa cccataaact gtccgtctga
     1501 gagcccaccc gcgagattga tgcgtttcca gttaaacaat tttagctttc tcgttcgcgc
     1561 gctgagctca cgaaattttc gactgttctt ctttggtcag gcagcgtcgc ttatcggcac
     1621 ctggatgcaa caaatcgcca tgcgctggct agtctatcgg ctcaccaagt cggagttgat
     1681 gttgggtctg gtgggatttg tcagcgacat cccgctattt cttctggtac cgttcgccgg
     1741 tgttttggcg gaccggctca aacgccatca aatactgttc gttactcagt tactggccgc
     1801 gattcaggca gcggtcctgg cactcctcgt catcactgaa tctgtcgccg tctggcatgt
     1861 aatcctcctc ggcgggatcc tcggtatcat cagcgccttt gatatcaccg cacgccaatc
     1921 ctttatcgtt gatatgatcg aaaataagga tgacctgcct aacgctattg cgctgaactc
     1981 tttcatcttc aacggcgcgc ttttaatcgg tcccgcagtg gccggcattc tgatcgcagc
     2041 cgtaggggaa ggactctgct ttgcgatcaa cagcctgagc taccttacgg tgattggcgc
     2101 gctgctgatg atgaagatac ccaaaaagaa gttttcttcc agcgcacttg gttttaaggc
     2161 ggcaatcatc gaaggcgcaa cttacacctt tgcatcggtg ccgattcgat cgatattgat
     2221 gtttgtcgcc gtagtgagtt tcgtcgccgc atcctacccc ctgctgatgc cggtgtatgc
     2281 ggaaagtacc ctgcagggcg gcccacgtgt tttcggttat ctcatgtcgg ctaccggggt
     2341 cggcgcgctt gccggcgcga tatttcttgc ttcgcgcaac aatctcaaag gcctcggaga
     2401 gatgatcgta atcgcggcga tcctctacgg cgttggttat tttgcaatgg cgctgtccaa
     2461 tacgctgccg atatccttga tcatcgcgct cttgctaggc ttctcgttga tgatgcagat
     2521 ggcatcaagc aacacgattg tgcagactat cgtcgacgat cagaaacgtg gccgggtgct
     2581 gagcctattc gtaatggcgc gccgcggcat cgagtcgtta ggcagcctgc tgttcggcgc
     2641 catagctgca tggattggaa cgcccggcac aatgatgatc gccggcgcga tctgtcttct
     2701 ggcaggggca gcttttgcca ctcaagtccc ctccatcaga gaagccggca attccgttgg
     2761 tgacaatagt gatccggcgg agcggcgtga aacaaattta tgagcggaga tcgctcaccg
     2821 tctgtttcct tttcgagcta tcctattcga gttcctcagt ccagagctag agtccagcag
     2881 tagatcagca ccgcggccgt ggcgctacca aagagccact gcactagcag tccaggccga
     2941 actggaaaaa atagccccac aggtattcct aacgcagcgc cgcagaaaaa gccgaacaga
     3001 tgggccagaa cgtccgtccg tccggtaccg gtgccgagca tcgcaagtaa gcctaatgac
     3061 gccgcgattg gaatccaggc gccacggcgc cgcgttgggt ctcgccgcct cctgatcaat
     3121 ccttgacttc cgagcacacc gactgcgcca aagattgcag tggaggctcc caccgcgatg
     3181 tgggaagatc cttgaaccaa ggagttgact aaatttccgc aagcgcccga gattacgagc
     3241 agagtgaatg cgagccccgc gcctacgata ctggaaagcg cagtgacaaa aatggctgcg
     3301 gccaccgcgt tgctaattag atggactaga tcagcgtgca gtgtcagcgc cgtgactgtc
     3361 cgccacaact cgccgtcaag tattcggctc gcttcggcgc tgcctcgctc gatccaaggg
     3421 acggcctctt tccatctaac agtaccggca tggcttaaaa tcagcacgcc tgcaacaaca
     3481 agccctgcga cggaatttgg caaatcatcc gagcgattct gctcctttgg aacaggaacc
     3541 ggattctcgt tctcataggc tgccaggcca gcaagcgcca tctccgcctc ttccgcagga
     3601 atgctgagaa ccatgcccct ttggtcgtga tgtaagctgg gcgataaacc ttgagttatc
     3661 agaaccagcg cccactcttc agcgagttta gaatcaggtg taactcttag aacaacttcc
     3721 gagcggtcca tcataagaac gcaactataa cccgggagcg aatcgccagc tcggagcctt
     3781 catgtcatga tcgacaagcg cgagcggttt tcagttagga gattttcgag tccgagacat
     3841 tgcgagcgtt ggctagttaa tctgttcggc cttgcgtgca atcgcactca acatgccgcc
     3901 gaataggatt cgatgcagag gatagacagc ataccaatag gcaagcccca ccaagcccac
     3961 gggatcgaag attgcattct gggttatccg ggtaccgctg ccatgcggtt ccaacacata
     4021 ttcaagccag gcgcggccgg gcaacttcat ttccgcgagc aaacgcaaac ggcggttggg
     4081 gtcgactgct tccacccgcc aaaaatccag agcatcgccg acccgcaact cttccgggtg
     4141 gcgacggccg cggcgcaggc ccacgccgcc gaccagcaag tcgaaccatc cgcgtaactg
     4201 ccagagccaa tttgcaaaat accaacccgt cgctccaccg agtcgacaaa tcacggcgaa
     4261 gacgcgttcg ctaggaacct tgacatcggt aacccgcgaa tcgacaagtc tattgccgaa
     4321 tcgcacgccg gcccacgtag gttgcgctcc tgcagccgag agcgaatcgg accagcgggt
     4381 cgcggcgaat tccaggtctt cgttgcgcaa ggccgccgca atcgctgcgg ccattccgag
     4441 cggccggatg tcaaaggcgg cgagcgctgc tgggtcgcgg acgatcgtcg gattccgaat
     4501 actgtcgacg agctttcgtc cagttcgggc ataaaccggc gtgattagcg ccagccacag
     4561 gcttgatagc ctaggagtga ttacaggaac tgaaatcata atgcggcgca gaccgcgttg
     4621 tcgcgcatat tccgccatga tgtcgcggta ggacactcga tcgtttccgc cgatttcgaa
     4681 gatccggctt tcggctctgg gcaattccaa agcgctggca aggtaatcca aaacgtcgcg
     4741 cactgcgatc ggttgcgcga tgatcgaaac ccatctcggc gtaatcatca ctggcaaacg
     4801 ttcgactaaa gcgcgaatca tctcaaagga taggctgccc gagccgataa caatagaagc
     4861 tcggaattcg atgaccggga ctccagaatc gcgcaggatc ttgccgactt cctgacggct
     4921 acgcaaatga gaagaaagcg cctcgccgct tcccaaaccg cccaggtata tgattcgacg
     4981 tactccagcg cggcgcgcgg cagcagcaaa gttgcgcgcg gcgatccggt cttgttcctc
     5041 gaaacggcca gcgctcccca tagaatgaac gaggtagtag gcctgatcca cgccgatcaa
     5101 agcggcatcg agagtctcag gggcaaacac gtccccggcg acgacttcgg tgtcccggcc
     5161 gatacgggaa cggagatact caggatgacg tgcgaggcag cggactcggt agccttttcc
     5221 ttccaggacc gtcagcaggc gtccaccgat gtacccggtt gcgccggcta tgaaaatgag
     5281 attacgatcc atctcctcgc ctgcagctca tccaccctag tcatgatgca acgagagttt
     5341 ctgtcgcttt aatgtttact cttacagaga aatcgctacc tgccaagtca atgcgccgta
     5401 agcttggaag gacgagaagg gaggactacc gcgggcccgc aaatttcaat caactgatgc
     5461 gtctactgcg gatggattta tctcatcagc ggctatttca accaatctgc ttctctgtag
     5521 gctctttctg ctcccggatg tagtggcacg tcaccgaact caaaggccga gcgaccctca
     5581 acccggagtg gctcgaagag atttttgagg cctctgaaga ggcgattcat gtttggcaga
     5641 ttgtcgagat tatcaacgat ggcccgcgcc atgttatgaa cgatgctttc cgacgtccgt
     5701 gcatgtgtca ccaaaacatt gaccaccccg agctgcgcga tatcctccac cactccacga
     5761 aaggcatcct tttcaataat gacacttcgg taaaatggca cttcgccgac aaccctgggc
     5821 agttggccag gcgcataggg gatgacgcga acgtcggccc gcaggctaag atcggtcata
     5881 acacgattgg gaatgggcgg ctggaactgc gcgtcgatat tgccggcgat caattcatca
     5941 gcgccttggg caaaactcat attcaatggc gtgaagctgt cgaacccgat tcccagcacg
     6001 gcgaaaatcg tttgcacgtg ttgttccatg ccgctgccct tcgtgccgat agcgatgcgc
     6061 ttacccttga actcggcgat gctttgaatc ggtgaatcgg cgcgcttgac aaaaaacatc
     6121 ggcccaacat tcgccggcgc aaccatgcgc aaatcaatct cacgtgtgaa tggcgccgta
     6181 ccctcttttg cccggccgat ccaattcgaa gccatgaaac cgaaatctaa ctcg
//
LOCUS       DASUTG010000589         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_388161, whole genome shotgun sequence.
ACCESSION   DASUTG010000589 DASUTG010000000
VERSION     DASUTG010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388161"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /gene="yqeC"
                     /locus_tag="VF089_13605"
     CDS             complement(<1..709)
                     /gene="yqeC"
                     /locus_tag="VF089_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HEX6175047.1"
                     /translation="MQLKEALGLRDGEMVSLIGAGGKTTTLLHLAKELRDAGKKVLVT
                     TTTKIFKPSRPHVDKLFIVEDVDALLCEAAKLDPPLIIGAGSKLDQEKLIGLPARWLD
                     EIATSNHFAAVLVEADGAASRLFKVPSEFEPVVPRTTALTIWVLSIKVVGKPLDGTSV
                     HRAETAAALSGMPLGTIVTEELILNLMRHPEGCLKGIPASSRRSAMINQADSPEEIQR
                     AKQLGRALLQLGFERVVV"
     gene            844..1335
                     /locus_tag="VF089_13610"
     CDS             844..1335
                     /locus_tag="VF089_13610"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX6175048.1"
                     /translation="MSRFFSIEVAKDYFNFASAHFLIFPDGQRESLHGHNYQVSVTLE
                     GEMDHAGVVLDFITFKPLVKQVCDALDHRTLIQTESPIIKVNRRGQEIEVRYKTQRLI
                     LPRQDVILLPLVNTSTELLAEHVANQLRREIRKKFPHSRIRNMEVGVEEARGQRGIYR
                     GEF"
     gene            complement(1455..2279)
                     /locus_tag="VF089_13615"
     CDS             complement(1455..2279)
                     /locus_tag="VF089_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6175049.1"
                     /translation="MNKVISHWPANRRMAVMITVMFEVWSEGKAPPYSPMTTALKPGT
                     PDLLGISWSQYGGNTGVWRLMRTFDECDVRGTVCLNAKCAEDFPHAVKQLHERGHEIS
                     AHSYTQDLVLPYLSAQEEKDVIQKCSRIIESVVGARPVGWFSPVAAPTVNTARFLAEE
                     GFLWHGDYNDSDLPYSMNMPAGRLVAIAHSDFTDNRTLRSSPRDFFNVYKDTFDFLYR
                     TESPSLINLTVHTHFGGRPLMAAMLSELLHYMKGFADIWFARHDEVARWVLEQSEA"
     gene            complement(2308..3252)
                     /locus_tag="VF089_13620"
     CDS             complement(2308..3252)
                     /locus_tag="VF089_13620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6175050.1"
                     /translation="MKYRILGRTGLRVSAVGLGTMVHAGHFGPMDDRESLSAIETAIE
                     LGVNFIDTSDAYGAGYSETLLGQALRGKRDKVILATKGGNVMVGPERGKRNFTPAYID
                     RVLDESLQRLQTDYVDLYQLHNPTVDVIEHGDVWEVLERAKKAGKIRHCGVSINTIEE
                     GIAAVNGGRSDTVQLEYNLLAQEPAEKIFPLAQKANVGIIARVPLRRGILTGKLTAAD
                     DGRFQGEDVRARSFKGEPFRRELEKAEQLRFLVDGNIKTLAQAAIAFCLAHPAVSTTI
                     PGARNAQQMRENAAGADVTIPPEHLKRIDELWRGSLSS"
BASE COUNT          778 a          949 c          837 g          814 t
ORIGIN      
        1 tgaccaccac ccgttcgaat cccagctgga gcagagctct gcctaactgt ttcgcccgct
       61 ggatttcctc cggactgtcc gcctgattga tcatcgctga tcttcgactc gacgctggaa
      121 tgcctttgag acaaccttca gggtgcctca ttaggttaag aatcaattct tcagtgacga
      181 ttgttcccag cggcatgcct gacagcgccg ctgccgtttc cgctcgatgg accgaagtac
      241 catccaacgg tttgcccact acttttatcg acaaaaccca gatcgtcaga gccgtcgtgc
      301 gcgggaccac cggctcgaat tcggacggaa ctttgaagag ccgcgacgcc gctccatctg
      361 cttccaccaa aaccgcagcg aagtgattgc tcgtcgcgat ctcatccagc caacgagcag
      421 gcaatccgat tagcttttct tgatcgagct tagaaccggc gccgataatc agcggcgggt
      481 cgagcttggc ggcctcgcag agtagcgcat cgacgtcttc gacgataaac agtttgtcaa
      541 cgtgtggcct gcttggttta aaaattttag tcgtcgttgt gaccagaact ttcttccccg
      601 catcgcgcag ttccttggcc aaatgcaaca acgttgtggt ttttccgccc gccccaatca
      661 gcgacaccat ctcgccgtcg cgcaggccga gagcctcctt tagttgcata ggttcctcac
      721 ctaactcttc caagctggag ttgcaagtta attgagaaaa aagagcgtat taaaaatcat
      781 cgtttcacgc gaatcgacaa ctgattgctt gaaacagtgg aggcttttgg ctaacgtccg
      841 aggatgtcac gttttttcag cattgaagtc gccaaggatt actttaattt tgcctcggct
      901 cattttctaa ttttcccgga tggccagagg gaatctttac acggccacaa ctaccaagtt
      961 tcagtgacgc tggaaggaga aatggatcac gccggggtcg tgctcgattt cattaccttc
     1021 aagcctttgg tcaagcaggt ttgcgatgcg ctggaccatc gcactttgat tcaaaccgag
     1081 agcccgatta tcaaagtgaa ccgccgcggc caagagattg aggtccgtta caaaacacag
     1141 cggctaatac tgccacgaca agacgtcatt cttctcccgc ttgtgaacac cagcaccgaa
     1201 ctgttggccg aacacgttgc caaccagctc cggcgcgaaa ttcgtaagaa gtttccgcac
     1261 agtcgcatcc gcaacatgga agtcggcgtc gaagaagcac gcggacagcg cggcatctat
     1321 cgcggcgagt tttgactgat tcgcttcgta ttccgtttag gtcatggttc agttttttgg
     1381 gatactagtc tctttgtgac agactggccg gcggtgaaaa atatacccgt accgtaagaa
     1441 gtaacgtgaa acttctacgc ttcagattgt tccagcaccc agcgcgcgac ttcgtcgtga
     1501 cgagcaaacc aaatatccgc aaaccctttc atataatgga gcagctcgct gagcatggct
     1561 gccattaacg gccgtccgcc aaaatgagta tgaaccgtca gattgatcag cgagggtgac
     1621 tcggtgcgat acagaaagtc aaaagtgtct ttatacacgt tgaaaaaatc tcgcgggcta
     1681 ctgcgaagcg ttcgattatc ggtgaagtca ctgtgcgcta tcgcaaccag ccggcctgca
     1741 ggcatgttca tcgaataagg cagatcactg tcgttataat ccccgtgcca gagaaatccc
     1801 tcttcagcga gaaatcgcgc ggtattgacg gtcggcgcgg ccaccgggct gaaccaaccc
     1861 accgggcgag ctcccactac gctttcgata atgcgactgc atttctgaat cacgtctttc
     1921 tcttcttgcg ccgaaagata cggcagcacg agatcctgag tgtaagagtg agctgagatt
     1981 tcatgcccgc gctcatgaag ctgttttacg gcatggggga aatcttccgc acatttggcg
     2041 tttaagcaaa ccgtccccct gacgtcacat tcatcgaatg ttcgcataag tcgccagaca
     2101 cccgtgttgc ccccatattg cgaccaggaa attcctaaaa ggtccggcgt cccaggcttt
     2161 agcgccgtgg tcatcggtga gtagggaggc gctttacctt cggaccagac ctcgaacatc
     2221 acggtgatca taacggccat tctacgatta gccggccaat gagaaattac tttgttcatg
     2281 aatcctccga ccggaaccaa tcagccgtca ggaactgagg ctgccgcgcc atagctcgtc
     2341 aatccttttt aaatgctcgg gaggaattgt cacgtctgcg cccgcggcat tttcacgcat
     2401 ctgctgggcg ttgcgagcgc ccggtatcgt ggtactgacc gcaggatggg ccaggcaaaa
     2461 agctattgca gcctgagcca atgtttttat gtttccgtct accaggaatc ggagttgctc
     2521 cgctttttcg agctcccggc gaaagggctc gcccttgaag ctgcgcgccc gcacatcttc
     2581 accctgaaat ctaccatcgt cagccgcggt tagtttcccg gtcaaaattc cgcgacgcaa
     2641 cggcacgcgc gcgataatgc ctacattggc tttttgcgcc agagggaata ttttttccgc
     2701 tggttcttgc gcaagaagat tatactccag ctgtaccgta tcggagcggc cgccgtttac
     2761 cgccgcgatc ccttcctcga tggtgttgat ggaaaccccg caatggcgaa ttttgccggc
     2821 tttcttggcc cgctcgagca cttcccacac gtcgccgtgc tcgatcacat cgacggtagg
     2881 attgtgcagt tggtacagat ccacatagtc ggtttggagc cgctgtaagc tttcgtccaa
     2941 gacccgatcg atataagcag gagtgaaatt tcgcttgcca cgttcgggac cgaccatgac
     3001 gttaccgccc ttggtcgcca gaatgacttt atcgcgctta ccgcgcagtg cttgtccgag
     3061 caatgtttct gaataccctg cgccataagc atcagaggta tcgataaaat tcactcctag
     3121 ttcgatagcg gtttcgatag cgctcaggga ttcacgatca tccatgggcc cgaaatggcc
     3181 ggcatgaacc atcgtaccta agccaacagc gctcacccgc aagcctgttc taccaagaat
     3241 tcggtatttc attcatcctc cggtttttgc gccaacataa aacacgtctg cagccatacg
     3301 caagatcacg aacgatgata gatagattcg gctggcagtg cgatgtctaa attgcttcgg
     3361 gtaactctcg ttgacaag
//
LOCUS       DASUTG010000590         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1128148, whole genome shotgun sequence.
ACCESSION   DASUTG010000590 DASUTG010000000
VERSION     DASUTG010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128148"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..753)
                     /locus_tag="VF089_13625"
     CDS             complement(106..753)
                     /locus_tag="VF089_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175051.1"
                     /translation="MDSREFAKTLEKQVADFCETLNSKLPAYSYIPLESDEARIKVMQ
                     SRLFNEIRAGEIFGGWLKTTPELDVKKTLAEAVHEEYVHAGYLEEALKAKGAVPYEYK
                     PLPAQMARFNAFEGLTDTVERIAAFPMAGEGVADYLIGKSLNVGTVPAWVTGPYQKIH
                     EDEEEHGNYPFEILVKYATTPEKQERAARAVAMSLTLRRQYFDNLDRWVLEDKLY"
     gene            1076..>2136
                     /locus_tag="VF089_13630"
     CDS             1076..>2136
                     /locus_tag="VF089_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX6175052.1"
                     /translation="MPKKSSSKGTRQGAATDRKSVHYNDQNVTIGTGGETHQIADGER
                     PVLTTQQGIPVADDQNSLRIGTRGPTALEDFHFREKLFHFDHERIPERVVHARGFGAH
                     GYFENYESLADITKADLFQRAGEKTPLFVRFSTVAGNKGSPDLARDVRGFAVKIYTKE
                     GNWDIVGNNIPVFFIQDAIKFPDVVHAAKQEPDRGFPQAQTAHDNFWDFISLTPESMH
                     MVMWIMSDRTIPRSFRFMEGFGVHTFRFINSDGKSTFVKFHWKPKLGLQSVVWNEAVK
                     INGADPDFHRRDLWNAIQAGNYPEWELGLQHFDEDFADKFEFDVLDATKIIPEEDVPV
                     RRVGRLVLDRCVDNFFAET"
BASE COUNT          491 a          565 c          559 g          521 t
ORIGIN      
        1 ctctcgtgcc gtgtaagaac ttctttaaag gtcaaaggtt cgttcttcgc agctcaaccg
       61 ttaccccttc tgttttcgcc cggaacatgc ttaaaaatgc caggcctagt acagcttatc
      121 ttcgagcacc cagcgatcaa gattgtcgaa gtactggcgg cgtaaggtca ggctcatcgc
      181 cacggcgcgt gccgctctct cttgcttctc cggtgtcgtc gcgtatttga cgagaatctc
      241 gaaaggataa tttccgtgtt cttcctcgtc ttcatggatt ttttgatacg ggcccgtaac
      301 ccaggctgga accgtgccga cattcaggct tttgccaatc aagtagtcgg caacgccttc
      361 acccgccata ggaaaagcgg caatgcgctc gaccgtatcg gtcaatcctt cgaaggcatt
      421 gaacctggcc atctgcgcgg gcagcggctt gtattcatag ggcaccgcgc cttttgcctt
      481 gagcgcttcc tcgaggtaac cggcgtgaac gtattcttcg tgcacggctt cagcaagagt
      541 tttcttgacg tccagttccg gcgtggtctt gagccatccg ccaaaaattt cgccggcgcg
      601 gatctcgttg aacagacggc tctgcatgac tttgatgcgg gcttcgtccg attccagcgg
      661 aatgtagctg tatgctggca gtttgctatt aagtgtttcg cagaaatcgg cgacttgctt
      721 ctcgagcgtt ttggcaaatt ctcgactatc catcaaggat tacccctaaa tttcccgtgc
      781 cgggcacgca atttagttct tgttatcagg attgttgtag gagcgtcaag caacagaagc
      841 actcatgcgc atcgagttcg ggctgtcgaa aggcattcgc tgtgagtgac cgaaaatagg
      901 tcagtggaaa attccgaatg ccccaatatt gagtcgcgcc gatcatcgaa gtatcgcaag
      961 acccaggaca gtagcctgca ctatgcatgg catagatgtt gcgccgaggc ttgtcgtcct
     1021 agccacaaat cattagctca tttcgtattt aagttgcctt aaacgaggag aaagtatgcc
     1081 taaaaaaagc tcatccaaag gtacgcgcca gggcgctgcc accgaccgaa agagtgtcca
     1141 ttacaacgat cagaatgtaa cgatcggtac tgggggagaa acgcaccaga ttgccgatgg
     1201 cgaaaggcct gtactgacta cgcagcaagg aattcctgta gcggatgacc aaaactccct
     1261 caggatcgga acgcgcggac cgaccgcgct ggaggatttc cactttcgcg agaaactttt
     1321 tcatttcgac catgagcgca ttccggagcg cgttgtccat gcccgcggtt ttggagcgca
     1381 cggctacttc gagaactatg agtcgctggc cgacatcacc aaagccgatc tgttccagcg
     1441 agcaggggaa aaaacgccgc tgttcgtgcg tttctcgacg gtcgcgggta acaaaggctc
     1501 gcccgacctc gctcgcgatg tgcgcggctt tgccgtgaaa atctatacta aagaaggcaa
     1561 ctgggacatc gtcggcaata atatcccggt gttcttcatt caggacgcca tcaaatttcc
     1621 agatgttgtt catgcggcca agcaagaacc ggatcgcggg tttccgcagg cacagacagc
     1681 gcacgataac ttctgggatt tcatatccct tacgccggaa agcatgcaca tggtcatgtg
     1741 gatcatgtcg gatcggacaa tccctcgctc attccgcttt atggaaggtt ttggcgttca
     1801 taccttccgc tttatcaatt cggatggcaa gtcgaccttt gtgaagttcc attggaagcc
     1861 aaagctcggc ttacagtcgg tggtctggaa cgaagccgta aagatcaatg gcgccgatcc
     1921 tgattttcat cgaagggatc tttggaatgc gattcaagcg ggcaactatc ccgaatggga
     1981 gcttggtctg cagcacttcg atgaagactt cgccgataaa ttcgagttcg acgtgctcga
     2041 cgccaccaaa atcatcccgg aagaggacgt gccggttcga cgggtaggtc gactcgtgct
     2101 cgaccgttgt gtcgataatt ttttcgccga aaccga
//
LOCUS       DASUTG010000591         8303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_536176, whole genome shotgun sequence.
ACCESSION   DASUTG010000591 DASUTG010000000
VERSION     DASUTG010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536176"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF089_13635"
     CDS             <1..738
                     /locus_tag="VF089_13635"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175053.1"
                     /translation="WHTGKHFHGEEAIYVESGEGFLILDEKRYDFFPGTILHIPYRSP
                     HQLFNTGNAPVGYLSGLAWHLEADVYMGKLEQLEECGENDPNILANFPKEESQYWPED
                     GRRISMHQKQHEKSDESKHGATYFLMGRSGKQNGFRATGVAISSIFVDLPHTKSHSHA
                     HPEAYLYALEGDGYSEIGGKRYDWNQGDAVHVPPGMLHHQHFNPFDRDMKELRFEFGI
                     RYWFVDQWQKGYTTVDKHLKATTHMDD"
     gene            870..992
                     /locus_tag="VF089_13640"
     CDS             870..992
                     /locus_tag="VF089_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175054.1"
                     /translation="MSEEETVGTEDEAYLPLRISEFQCEIRSGKIIEQETRAER"
     gene            1036..1302
                     /locus_tag="VF089_13645"
     CDS             1036..1302
                     /locus_tag="VF089_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175055.1"
                     /translation="MTLQNPKNGAPKMMLGFIVRRCAADIGHTPSPDEFAAWANARRQ
                     DGQEYCLFGKPISPSIAKVMFQQPGRLVTVNGDGFEKHWRGPRG"
     gene            1597..2607
                     /locus_tag="VF089_13650"
     CDS             1597..2607
                     /locus_tag="VF089_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175056.1"
                     /translation="MSIKRLSSIAAGIVLVLSAFEAHAQRKPEKLRIGYAARAVAHSV
                     PFVAKEAGFFFEEGIDAEVVRTAGSIAPMALIANEVDLAIMSAYLMIPVSERNRDIVM
                     LGGFSRYASMVLVARPEIKSAQDLKGKIVGIQRPGDAYEKSLRFSLRHLGLDADKDVT
                     MLALGTNDVMWGALQTGRVAATILSPPGTLFARKAGMNFMVDLTDLKLEYQGSTIATR
                     RSLVQKNPALATRALRAIVKGVHLFKTRKEDTVRILGKFLGTSDREALEESWEYAAKM
                     PAKPYAVESAVQAVLDHLAEAQPKFAQYRPADFIDGRVLSEIDKSGYIDKLYGAHGTQ
                     GR"
     gene            2641..4680
                     /gene="uvrB"
                     /locus_tag="VF089_13655"
     CDS             2641..4680
                     /gene="uvrB"
                     /locus_tag="VF089_13655"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6175057.1"
                     /translation="MLGNRQPGQFRIISGFSAQGDQPSAIEKLYSGIQQGKRHQVLLG
                     VTGSGKTFTIANVIEKANKPTLVLAPNKTLAAQLYNEFKELFPENAVRYFVSYYDYYQ
                     PEAYVPSTDTFIEKDSAINDEIDKLRHSATKALLERRDTLIVASVSCIYGLGSPEAYF
                     ELMVYLEEGARVERDQMLRKLVDIQYQRSDFDFHRGTFRVRGDCVEVFPAYEDKSALR
                     VEFFDDTVEGIYEIDPLRGKVQRRVDKAMVYPASHYATTESRMKQATRDIRVELRERL
                     KELRDQNKLLEAQRLEQRTLYDLELLEEMGFCPGIENYSRHLTGRNPGEPPPTLLSYF
                     PDDFLLVIDESHVTVPQVGGMYRGDRSRKETLVEYGFRLPSALDNRPLNFGEFEACLN
                     QVIYVSATPSNFELEKANGVVVEQVIRPTGLIDPEVTVRPARSQVDDLLSEVRKRVEC
                     KERVLVTTLTKRMAEDLTDYYQDLGVKVRYLHSDIETIERVEIIRQLRMGVFDVLVGI
                     NLLREGLDLPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLHGKVILYADSMTDSM
                     QRAIDETNRRRSLQTTFNTENGITPQTIIKALGAPLVKIYEADYVEIPLAADGPTRYA
                     PADLTRKIKQLRKEMKQASGKLEFERAAELRDQIRELELRELQLRDGGLSAAQEL"
     gene            4926..6758
                     /gene="uvrC"
                     /locus_tag="VF089_13660"
     CDS             4926..6758
                     /gene="uvrC"
                     /locus_tag="VF089_13660"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6175058.1"
                     /translation="MTNLPSRPGVYLMRDGAGKVIYVGKAKDLRARVRSYFNRNDGRS
                     QIEFLVRRVENLETLVTSNDKEALILENNLIKQYKPRYNIRLKDDKSYLSIKVTTKDA
                     WPRILTTRKIVKDGSRYFGPFSSAVAARETVDIIEKHFLLRNCTDHNFKNRSRPCLQY
                     EIKRCLAPCVLAVDPEEYREQVRQASLFIEGRQHELLAELRQRMAAKAETMEFESAAK
                     IRDQIQAVEKTLEKQRMVSHWGADQDIFGLYREGGYIEAQVLFVRQGKLTGTQAYALE
                     DLDFSDEEIMGSLLTQFYQGQRFMPDEILLPVELEDRDTRAEYLSERKGKNMSIHVPQ
                     KGDKRKLVEMAQQNARQSFTERHDQEKAREKMLQDLQEQLHLKRYPQRIECFDISMIH
                     GAYAVGSQVTFTGGEPDKAHYRHYRIRTIDPSGGGDDFGMMLEVLKRRFTRGKREEEM
                     PNLVVVDGGKGQLAMALAAMREVGVEGIDVVGLAKMRVQSAPRSAEIERSEERVFLPG
                     QSNPVTLRRNSNPLFLLQRVRDEAHRFAITYHKKLRSKQTLSSALDRIPGIGAARRRA
                     LLRAFGSVKGVEQASLDELMQIPGMNPKLAQQLWQVFHSAPSPQ"
     gene            6768..>8303
                     /locus_tag="VF089_13665"
     CDS             6768..>8303
                     /locus_tag="VF089_13665"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX6175059.1"
                     /translation="MAPSSQQLAPTFPKAIHPSIWLFVLTGSLLLGQSIAVLPVVLSA
                     DLLFLLLVPLVLLIKPIGRLRWLLGSITGADLTIQRNTATINYPAWALLTIVAGLMFA
                     LGYSRHQQLLYPTFPVNHLRSVMNENNDLYLEGVLLHEPEKLPNRSRWYLRIERIWQP
                     TGAEEISGDLLLNVRRLRREWHYGDRIRAYIRPTVPKDSGNPGGFNYATYLSRRQIYA
                     IGFLESDEKIELVERAPDRLRGAVENLRRRIRRYIESYFTPESGALMKALVVGDMGEI
                     SKKTRSDFTAAGVNHVLSISGLHVAMLGLVVFWLVRWAGSFNLYLLLRCNLIKIATFC
                     SFVAVIFYTALAGAMVPTVRSAIMIGVYELAVLLDREEEVLTSLTFAALLIALVWPGV
                     IADISFQLSFLAVFSIAWGMRQVFRAVAAAEKDQLPQERSRIKPKLRKIGMHLAVPLL
                     ATIGTGPLIAHYFGHLSLAGFVSNPIVVPLVGFVVVPLGLSISFISLAWPELVAPLVS
                     LTEW"
BASE COUNT         1890 a         2185 c         2369 g         1859 t
ORIGIN      
        1 tggcataccg gcaagcactt tcatggcgaa gaggcgatct atgtcgagag cggcgaaggc
       61 tttctgattc tggatgaaaa acgctacgac ttcttcccgg ggacgatttt gcacattccc
      121 taccgttcac ctcaccaact cttcaatacc ggaaacgcgc cagtgggtta cctttcaggg
      181 ctagcttggc atctggaagc tgacgtttac atgggcaagt tggagcaact cgaagaatgc
      241 ggcgagaatg atccaaacat cttagcgaat tttcctaaag aagagtctca gtactggcct
      301 gaagacggac gccgcatctc aatgcaccaa aaacaacacg aaaagtcgga cgaatcgaaa
      361 catggcgcga cttattttct gatgggtcga agcggtaagc agaacggatt ccgagctacc
      421 ggtgtggcaa tcagctcgat ctttgtcgat ctgccgcaca ctaagagtca cagccatgct
      481 catccggaag cctacctgta tgcgctggaa ggtgacggct attcagagat cggcggcaaa
      541 cgttatgatt ggaatcaggg cgatgcggtg catgtgcctc cgggcatgtt gcatcatcaa
      601 cacttcaatc ccttcgaccg ggatatgaaa gagctccgtt tcgagttcgg cattcgttat
      661 tggttcgtcg atcaatggca gaagggctac accacggtgg acaaacactt gaaggcgacg
      721 acccatatgg acgactaaac gattgtcaga tgagagggct tgacggcggg ctaacgtatc
      781 gttgcccgcc gtttttcttt aaacgactag atcagggatc gtcaaatgtg gtttcggttc
      841 agcccggatg ggttagttga acctaagtca tgagcgagga ggaaaccgtc ggcaccgaag
      901 acgaagcgta tctgccgctc agaataagcg agtttcagtg cgaaatccgt tcaggcaaaa
      961 ttatcgaaca agaaactcgc gcggaaagat aaaactttct tgcaacaaat aatcagatct
     1021 tttatagagt aatcaatgac actacagaat cccaaaaacg gcgctccgaa gatgatgctg
     1081 ggcttcatcg tgcgccgttg cgcggccgat atcgggcata cgccaagccc cgatgaattc
     1141 gccgcttggg ccaacgctcg tcgccaggac ggacaagagt attgcctgtt tggcaaaccg
     1201 atctcaccct ccatcgccaa agtcatgttc cagcagccgg gccgtttggt gacggtcaat
     1261 ggagatggct tcgagaaaca ttggcgcggc ccccgcggct aaattttggg cctcctgcac
     1321 ttttgccaat ttgggctggc gcattcactg cacaactccc cagatctaag tcgttatggc
     1381 gctgacggcg ttggccgtga tttctgacct gatcagcata gacgcaatcg aaacaattcc
     1441 cggttcaagg gttcaccgcg atcgctgcct ggggtcgtgg cgttacggag ccagctaacg
     1501 ccgaatagtc aaagttgatc ggtctataac cacctgatat tttccccggc tatgcagatg
     1561 atgcttttta aacgtgtctg cggggaggga tacgcaatga gtatcaaacg gctttcttct
     1621 attgccgccg gcattgtctt agtcctatcc gccttcgaag ctcatgccca gagaaaacct
     1681 gaaaagctcc ggatcggcta cgccgcgcgc gcggtggcac attcggtgcc ttttgtcgcg
     1741 aaagaggcgg gatttttttt cgaagaaggc atcgacgccg aggtggtcag aacggcaggg
     1801 tcaatcgcgc cgatggcgct gattgccaac gaggtcgatc tcgcgatcat gtcggcatat
     1861 ttgatgattc cagtctcgga acggaatcgc gatatcgtca tgctgggagg cttttcgcgc
     1921 tatgcctcga tggtgctggt tgcgcgtccc gagatcaaga gtgcgcagga tctcaagggt
     1981 aaaatcgtcg gcatccagcg tccgggcgac gcctacgaaa aaagcctgcg ctttagcttg
     2041 cgccatctgg gtttggacgc tgacaaggac gtgacgatgc tggctctcgg caccaacgat
     2101 gtcatgtggg gagctctgca aaccggcagg gtagcggcaa ctattctctc gccgcccgga
     2161 actttgtttg cgcgcaaggc cggcatgaat ttcatggtgg atctcacgga tctaaagctg
     2221 gaataccaag gttcgacgat tgcgacgcgg cgctcgctgg ttcaaaaaaa tccggcgctg
     2281 gcaactcggg ccttgcgagc gatcgtcaaa ggcgtccatc tgtttaagac ccgcaaggaa
     2341 gatactgtgc gcattctcgg caagttcctt ggcaccagcg atcgcgaggc gctggaagaa
     2401 tcgtgggaat atgccgcgaa gatgccggcc aagccttatg ccgtggagag cgccgttcag
     2461 gcagtgctcg atcatttggc ggaagcgcaa cccaagtttg cgcagtatcg acctgctgat
     2521 ttcatcgatg ggcgcgtact cagcgaaatc gacaaaagcg gctatatcga caaactctac
     2581 ggcgctcatg gaactcaagg ccggtagcag cgcgttgatc taattgctat tttgtttgcg
     2641 atgttaggaa acaggcaacc aggacaattc cgaatcattt ccggattcag cgcacagggc
     2701 gatcaaccta gcgccatcga gaagctttat tcaggcattc agcagggaaa aaggcatcag
     2761 gtcttattgg gggtcaccgg ttcggggaaa acgtttacca tcgccaacgt tatcgagaag
     2821 gccaataaac cgactctggt gttagcgccg aacaagacac ttgccgccca gctttacaac
     2881 gaattcaaag aattattccc tgaaaacgcc gtccgttatt tcgtcagcta ttacgattat
     2941 taccagccgg aggcgtacgt gccgtcgacg gataccttca ttgagaagga ctccgccatc
     3001 aacgacgaaa tcgacaagtt gcgtcactcg gcgactaagg ctttgctcga gcgacgcgac
     3061 acgctgatcg ttgccagcgt ttcatgcatc tatggtttgg gttcgccgga ggcctatttc
     3121 gagttgatgg tttatctcga agaaggcgcc agagtagagc gtgatcagat gctgcgcaag
     3181 ctcgtcgaca tacaatatca gcgcagcgat tttgattttc atcgagggac tttccgggtg
     3241 cgcggcgact gtgtcgaagt attccccgct tacgaagaca agtcggcgct acgagtcgag
     3301 tttttcgatg acacagtgga aggcatctat gagatcgatc cgctgcgcgg caaagtgcaa
     3361 cggcgggtgg ataaggcgat ggtgtatccg gcgagccact acgcgaccac ggaaagccgc
     3421 atgaaacagg cgacgcgcga tatccgtgtg gagctccgtg agcgtttgaa ggagttgcgc
     3481 gatcaaaaca agttgcttga ggctcaacgg ctggaacagc gcacgttata cgatctcgag
     3541 ctcctggagg aaatgggttt ctgtccggga attgaaaact attcgcgtca tctgaccgga
     3601 cgcaatcccg gcgagccgcc gccgaccttg ctcagctatt tccccgacga tttcctgctg
     3661 gtgattgacg agagtcacgt gaccgtgccg caggtgggtg gtatgtaccg cggcgaccgc
     3721 tcgcgcaaag agactctggt cgaatacggc tttcgcctgc catcggcttt ggataaccga
     3781 ccattgaatt tcggcgagtt cgaagcgtgc ctgaatcagg ttatctacgt gtcggctacg
     3841 ccgtcgaact ttgaacttga aaaggccaat ggcgttgttg tcgagcaggt gatccgcccc
     3901 acgggattga tcgacccgga ggtgactgtt agaccggcgc gtagccaggt cgacgatctt
     3961 ttatccgaag ttcgaaaacg agtggagtgc aaggaacggg ttctcgtcac caccttgacc
     4021 aagcgcatgg ctgaagattt gaccgactac tatcaagatc tcggggttaa ggtgcgctat
     4081 cttcattccg acattgaaac gatcgaacgt gtcgagatca tcagacaact gcgcatggga
     4141 gtttttgacg tgctggtggg gatcaatctt ttacgcgaag gactcgatct gcccgaggtc
     4201 tcactcgtcg ccatcctgga tgccgacaag gaaggatttc tgcgctccga gcgctcgctt
     4261 atccaaacca tcggccgagc cgcccgcaac cttcatggca aagttatcct ctacgcggat
     4321 tctatgacgg attcgatgca gcgagccatt gatgagacca accgccggcg cagtttacag
     4381 acaacgttta atactgaaaa cggcatcacc ccacagacga tcataaaagc tctcggagct
     4441 ccgctggtga aaatctacga ggcggattat gttgaaatcc cgctcgctgc tgatggcccc
     4501 acacgttatg ctcccgccga tctcacgcgg aaaatcaaac agctcagaaa agaaatgaag
     4561 caggcatccg gtaagcttga gttcgaaagg gccgcggagt tgcgtgatca gattcgcgag
     4621 ctggaacttc gcgagttgca gctgcgcgac ggcgggctgt cagcagccca ggagttatag
     4681 agtcgctccg gggcgattcg aagcgcgcgc ggaaccagga cgcgtgcagg gtctgccgat
     4741 aacgccgcct ccttcaatca accgcactcg ggcaaagaat tccggctgca tcaattctgc
     4801 tcgtaagcaa acggcggcga tgaagactat gaggagtcca ggtagatgat cggcttgggc
     4861 acagttctcg cccggaaccg gacccaactg atatattgat agcgatgctt tccgtggatg
     4921 aaaaattgac caaccttccg tctcgtccgg gggtctatct gatgcgcgac ggggcaggaa
     4981 aggtgatcta cgtcggcaag gccaaggact tgcgcgcgcg ggtgagatcg tacttcaatc
     5041 ggaacgatgg acggtcgcag attgagttcc tcgtcaggcg ggtcgaaaac ctcgagactc
     5101 ttgtcaccag caatgacaag gaagctctca tccttgaaaa caatctcatc aagcaataca
     5161 agccgcggta caacattcgt ctcaaggacg acaagagtta tctaagcatc aaggtgacta
     5221 ccaaggacgc atggccgcga attctcacca cgcgcaagat cgtcaaggac ggcagccgct
     5281 atttcggtcc gttctcgtcc gcggtcgccg cgcgtgaaac ggtcgacatt atcgagaagc
     5341 atttcctttt gcgtaactgc acggatcaca acttcaagaa tcgcagccgg ccctgcttac
     5401 agtacgaaat caagcgttgc ttggcgccgt gcgtcctggc agtcgatcca gaggagtacc
     5461 gcgagcaggt gcgtcaggca agcttgttta tcgaagggcg gcaacacgaa ttgttggcgg
     5521 aacttcggca gcggatggcg gccaaagccg agacaatgga atttgaatcg gcggctaaga
     5581 tccgcgatca gatccaggcg gtcgagaaaa ctctcgaaaa acagcgaatg gtttctcatt
     5641 ggggcgccga tcaggatatc ttcggccttt accgcgaagg gggttacatt gaggctcagg
     5701 tgctcttcgt gcgccaaggt aaactgaccg gaacgcaagc ctatgccttg gaggatttgg
     5761 atttttccga tgaggaaatc atgggctcgt tgctgacgca attctatcag ggacaacgct
     5821 tcatgccaga cgaaattttg ctgccggtgg aattggaaga tcgggatacc cgggccgagt
     5881 atctgagcga gcgcaaaggc aagaacatga gcattcacgt gccgcagaag ggtgacaagc
     5941 gcaagttggt cgaaatggcg cagcagaatg cgcggcaaag cttcaccgaa cgtcacgatc
     6001 aggagaaagc ccgtgagaag atgcttcaag acctgcagga gcagctccat ttgaagcgct
     6061 acccgcaacg catcgagtgt ttcgatatct cgatgatcca cggcgcctat gcggtcgggt
     6121 ctcaggtgac gttcactggc ggtgaacccg acaaggcgca ctaccgccat taccgaattc
     6181 gcactatcga cccgtccggc ggcggcgacg atttcggcat gatgttggaa gttctgaaac
     6241 gccgctttac tcgcggcaag cgagaagaag agatgccgaa cctggtggtg gtcgatggcg
     6301 gtaaaggaca attggccatg gcgctggcgg ctatgcgcga agttggagtc gaagggattg
     6361 atgtggtcgg tctggccaaa atgcgcgtgc aatctgcgcc tcgcagcgct gagatcgagc
     6421 gatcggagga gcgcgtcttc ctgcccggac agagcaatcc tgtgacgcta agacgtaatt
     6481 ctaacccctt gtttctcttg cagcgagtgc gcgacgaagc acaccgcttc gcgatcacgt
     6541 accacaagaa actccgttcc aagcaaaccc tctcctcagc cttggaccgt attcccggga
     6601 tcggggcggc gcgacggcgc gccttgctgc gcgcgttcgg cagcgtcaaa ggggttgaac
     6661 aggcgagcct ggatgagtta atgcagatcc caggcatgaa cccaaagctc gcccagcaac
     6721 tctggcaagt atttcactcc gctccatcgc cgcagtgaga gcggattgtg gcgccatctt
     6781 ctcagcaact cgcgccaacg tttcctaaag ctattcaccc ctcaatttgg ctgtttgtgc
     6841 tgaccggttc cctcttgctg ggccagtcga tcgccgtttt accagtcgtt ctctccgccg
     6901 atctgttgtt cctgctgttg gttcctttgg tcctgctcat caagccgatt ggtagactca
     6961 ggtggctact gggctcaatc actggtgccg atctaacgat tcagcgcaat acggcgacga
     7021 taaactaccc agcctgggcg ctgctcacga tcgtcgcggg attgatgttt gcgctaggct
     7081 actcccgtca tcagcagttg ttgtatccaa cttttccggt gaatcacctt cgctcggtca
     7141 tgaatgagaa caacgatttg tatctagaag gagtgctgct gcatgaaccg gaaaagctcc
     7201 cgaaccggag ccgctggtat ttgcgtatcg aacggatctg gcagccgacg ggtgccgagg
     7261 agatctcggg cgatctgctg ttgaatgttc gtcggcttcg gcgtgagtgg cactacggcg
     7321 atcgcatccg cgcgtacatt cggccaaccg tgcccaaaga cagcggcaat cctggcggct
     7381 tcaattacgc cacctattta tcgcgacgtc agatttatgc catcggattt ctagaatccg
     7441 atgaaaagat cgagctggtg gagcgcgcgc ccgatcgatt gcgcggtgca gtggaaaacc
     7501 tgcgtcgaag aatccgccgc tacatcgaaa gctactttac tcccgaatcc ggcgccctga
     7561 tgaaggcgct ggtggtcgga gacatgggag agatttcaaa gaagacgcgc tccgacttta
     7621 cggccgcggg agtgaatcac gttttatcca tctccgggct gcatgtggcg atgctcggtc
     7681 tggtggtttt ttggctggtg cgctgggccg gttcgttcaa tctttatttg ctgttgcgct
     7741 gcaatttgat caagatcgca accttttgct cgtttgttgc ggtgattttc tacaccgcgc
     7801 tggcgggcgc gatggtgccg acggtgcgtt cggcaattat gatcggcgtt tatgaattgg
     7861 ctgtgttgtt ggaccgagag gaagaggttt taaccagtct gacgtttgcc gccttgctaa
     7921 tcgcgctggt ttggcccggc gtgatcgccg acatctcctt ccagttatcg tttcttgcgg
     7981 tcttttccat cgcgtgggga atgcggcaag tttttagggc tgttgccgcg gccgaaaaag
     8041 atcagttgcc gcaggagcga agccggatca agccaaagct gcgcaaaatt ggaatgcacc
     8101 tcgctgtgcc gctcctggcg accatcggca cgggaccctt gatcgcgcac tatttcggtc
     8161 atctctcctt ggccggattc gtttccaacc cgatagtcgt gccgttggtc ggctttgtcg
     8221 tcgttccgct ggggctgtcg atcagtttca tctctctggc gtggccggaa ttggttgcgc
     8281 ctttagtatc gcttaccgag tgg
//
LOCUS       DASUTG010000592         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_388459, whole genome shotgun sequence.
ACCESSION   DASUTG010000592 DASUTG010000000
VERSION     DASUTG010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388459"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VF089_13670"
     CDS             complement(<1..883)
                     /locus_tag="VF089_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175060.1"
                     /translation="MVTTDIGLLSIALPVIITDLHADLALAGWIALIYALVTASLYLP
                     CGRLSDMFGRAKVYRVGFFLYAATSLIAGFAQDAWQLIFFRGLQAIGSALIMTNSFAM
                     VAAIFPPQERGRAMGIAGGTVSALGYTLGPVIGGVLTYALGWRANFFLTSLLSFIGFV
                     AACYLLRDDSDGLDRHGPKESFDFAGAVSFALAIGALLLGLTVGQRAGWTSVLILTEL
                     GLGLATLAFFIWWEAGNRFPLLDLRLFQIRAFSYGNIARLISFMVISLNNLVMPFFLQ
                     LAMGLDPLRAGLLVAPTP"
     gene            complement(1125..1919)
                     /locus_tag="VF089_13675"
     CDS             complement(1125..1919)
                     /locus_tag="VF089_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6175061.1"
                     /translation="MKEIPALELRDVSYIGNGKTILRDINWSVRSGEHWAILGPNGSG
                     KTTLLKIACGYIWPNAGGEVYRRGRLLTNLPELRKSIGWVTSTLATQIPEHEKVIRTV
                     LSGRFAQIGLLEQFGGRAGARDYAAARRYLVEMGCDHLVEQEFGLLSQGEQQKVLIAR
                     ARMTRPYLIVLDEPCAGMDPGARENFLATLGRVTRQAKIPALIYVTHHVEEILPIFKK
                     TLILKDGRIIHCGNTDRVLERDILQELYGVSLKIIKKKGRCWPMVQ"
BASE COUNT          538 a          612 c          569 g          421 t
ORIGIN      
        1 aaggcgtcgg cgcgacgagg agcccggcac gcagtggatc caaccccatg gcaagctgca
       61 aaaagaacgg catcaccaga ttattgaggc ttataaccat gaagctgatc aagcgtgcaa
      121 tgtttccata actaaatgcg cgaatctgaa aaagccgcaa gtcgagtagc ggaaagcgat
      181 tgcccgcctc ccaccagatg aagaaagcta atgtggccag accaagaccg agctcggtca
      241 ggatcagaac gctggtccaa cccgcccttt gccctaccgt taggcccagc agcaacgcac
      301 cgatagccag cgcgaaactc accgcgccgg caaagtcgaa ggattccttg ggaccgtgcc
      361 ggtccagccc atccgagtca tcgcgcagca gatagcacgc cgccacaaag ccgataaacg
      421 acagcaacga tgtcagaaaa aagtttgccc gccagcccag cgcataagtg agtaccccgc
      481 caatcaccgg acccaatgta tagcccagcg ctgaaaccgt gccgccggca attcccatgg
      541 cgcggccgcg ctcttgcggc gggaaaatcg ccgccaccat ggcgaagctg tttgtcataa
      601 tcagcgcgct gccgattgcc tggagcccgc gaaagaaaat cagttgccaa gcatcctgcg
      661 caaaaccggc gatcagcgaa gtcgccgcat agagaaagaa tcctacccga taaactttgg
      721 cccggccgaa catgtcggag agcctgccgc agggaaggta aagagacgcc gtcaccagcg
      781 catagatcag tgcgatccaa ccggccaaag ccagatcggc gtgcaaatcg gtgatgatga
      841 ctggcagcgc gatgctgagc agcccgatat cggtggtcac cataaacgtt cccagtgaca
      901 caaccgccaa tgaccaccaa cgatggcgcc gcgtgaccgg ttgataattg tggggcgaca
      961 tgggtgcgaa gaatgtgact tactaaggcg ttccggatga ttgactgtgt ggtgcgacta
     1021 aaagaccaaa ccgttggcta cgcaacgggt ttctgccgcg atatccaagt gcacaatata
     1081 ttctaggctg acggatggga tcgataagga gtgctaggct gccatcactg gaccatcggc
     1141 cagcaacgcc ctttcttttt tatgatcttc aaggatacac cgtagagctc ctgcaaaata
     1201 tcgcgctcta acacgcgatc agtattgccg caatggatta tccggccatc cttcaagatc
     1261 aaagtttttt taaatatcgg cagaatttct tcgacgtggt gggtgacata gatcagtgcg
     1321 gggatttttg cttgcctagt gactcggcca agcgtcgcta aaaagttttc acgcgcgccc
     1381 ggatccattc cggcgcacgg ctcatcgaga acaatcagat agggccgggt catgcgagca
     1441 cgcgcaatca gtaccttctg ctgctcaccc tgagataaga gcccaaattc ctgctcaacc
     1501 aaatgatcgc aacccatctc gaccaagtag cgccgcgcag cggcgtagtc cctcgccccg
     1561 gccctgccgc caaactgttc gagcaggccg atttgtgcga accttcccga taagacagtt
     1621 ctaataacct tttcatgttc cggtatctgc gtcgccaagg tcgatgttac ccagccgatg
     1681 ctcttgcgca attcaggaag attcgtcagc aatctccccc gccggtacac ttcgccgccg
     1741 gcgttgggcc agatatagcc acaagcgatc tttaacagcg tggtcttgcc ggaaccgttg
     1801 gggcccagaa tggcccaatg ctctcccgac cgaaccgacc agttgatatc ccggaggatt
     1861 gttttgccgt tgcctatgta tgaaacgtcg cgaagttcga gcgcgggaat ttctttcacg
     1921 cggttatgcc tcgtcacaga actaatgcac ttttaccgaa agtaaaagcg gccggcatca
     1981 gacttggctt ttctcgggac ggcagttatg tctgatgctg taataggaga tcgggaagac
     2041 caacacatca tcgcgttcgg agtgagccac agagcacgat tagcagggca gcggttttta
     2101 agccgctgtg attgatcaag tgaggaggcc tggatgacta
//
LOCUS       DASUTG010000593         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1128604, whole genome shotgun sequence.
ACCESSION   DASUTG010000593 DASUTG010000000
VERSION     DASUTG010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128604"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..537)
                     /locus_tag="VF089_13680"
     CDS             complement(298..537)
                     /locus_tag="VF089_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175062.1"
                     /translation="MAMYNVCAVCNACGDLHTTGITVSLNGGPTSKQSIEEAFANKDP
                     PPNIAELKEKRVHCPKIGRQYAQRDDRKIFLIPMG"
     gene            complement(1202..1576)
                     /locus_tag="VF089_13685"
     CDS             complement(1202..1576)
                     /locus_tag="VF089_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175063.1"
                     /translation="MGEKILIVEDHHATRKNVSRFLQSQGYSVFEASDGLEALNRLNE
                     DSVDLVISDFVLPKIHGLALVREIRSRWPAIGVIIMSAYMGQEAGKVILDREAEFVAK
                     PLQLSHLAAKVYGVLLRKQMNH"
     gene            complement(1709..2539)
                     /locus_tag="VF089_13690"
     CDS             complement(1709..2539)
                     /locus_tag="VF089_13690"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX6175064.1"
                     /translation="MNRRNFIESSAIAAGAVTVSLGCSLRVPPVTDQTSHRPWPLPKS
                     RWVMFMRWHDLLFLHWPIKPEYIRPLIPMALELDTFDGWCWIGIVPFHMSGVRPRYVP
                     LPLALPELNVRTYVKTAGKSGVWFFSLDAASWLAVRVARWLGLPYYDARITVKSAEDT
                     IRYDSVRTHKNSPRAEFRADYRPIGAVYRATAGTLDHWLTERYCLFAALDPDRVVFGE
                     IHHPQWVLQPAEANLDVNTMAQSTGIELPERKPLAHFARLQEVVAWPIVPFERMMPAI
                     "
     gene            complement(2594..3355)
                     /locus_tag="VF089_13695"
     CDS             complement(2594..3355)
                     /locus_tag="VF089_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6175065.1"
                     /translation="MTIHEEPIEIAVDDQQIEARLIAGVSAGPAVLFVHGWGGNQQQY
                     LNRARAVAELGFICLTFDLRGHARSDGQRELVSREDNLRDLLAAYDVLASQPGCDRAS
                     LGVVGSSYGAYLAAILTSERAVGWLALRAPALYNDAGWELPKRKLHEDPDFRAYRKRV
                     VAPEENRALRACAAFLGDVLVVESEQDDVVPHPVIANYLAAFTRARSLTHRLIKYADH
                     GLTDVSWQRTSTSFLLQWLADRVIEASAGVTYARE"
     gene            complement(3318..>3838)
                     /locus_tag="VF089_13700"
     CDS             complement(3318..>3838)
                     /locus_tag="VF089_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX6175066.1"
                     /translation="ADLLATYYGRQLLTQDNQGANVYGGSDLIFVRGDYDDLLSLELP
                     PAARVAIKSARTYEAAASRAFAGWLASRRNYDVAHVVDDDGRRSCGVLEQSWRIGGAS
                     AAEIAALEAFRADPSLRSVRAASIESYGEHPVPPDAVVYFQGIDDQVGPITKYSQVKV
                     YDNSRRADRDRG"
BASE COUNT          870 a         1112 c         1009 g          847 t
ORIGIN      
        1 ctatgtccgt ccatcgtaga cctccattca tggtgtcgga aggagcatac atacgcgcga
       61 caaaacgccg ctttcgtcgc cagtcgcaat tttcgcgcct caacgtgtag aaaaaaggta
      121 ggctcggctc ttcgctaaaa catgcagcta gtgtaagaat gagttctgga aaatcgatgc
      181 gcagcagtgg gagatccgcc gtgggtgcgg gaggccagcg attatctgac ggttgagctt
      241 cctcccgctg aagccaaccg cttagagtgc cagtcccaaa ttgattaaag cagccgttca
      301 gcccattgga atcaggaata tcttcctgtc atctctttga gcatactgcc gtccaatttt
      361 ggggcaatgc acccgctttt cctttaactc ggcaatatta ggaggaggat ccttattggc
      421 aaaggcctcc tcgatacttt gtttgctggt cggccctcca ttcaggctaa cagtaatccc
      481 agtcgtatgc agatcaccac aggcattgca cacggcacag acgttataca tagccatgct
      541 ggtatcctcc cgtttgcaat cccagctaag gaaacatcgc ctgctagttc gccgcctttg
      601 ctaatgcttt gctccgttac ccccttttta gagaacgttc gtctaaatca acttcctgcg
      661 ttttgagtcc aagctcttgt gccttctgat tgcacggatg acacacaagc atattgagca
      721 gtatgccgcc cggatcctca ctgatcacac ggtaagcttt gctagtgagc gcccgctcac
      781 agtgatcgca aaacaactcc ctggccatgt gagtgcaggg agcaaggcac atgccatcaa
      841 gctccaagaa aaaacttaga taaaaatttg cttgtgttgc cgagaatact tgaaacgctc
      901 tagttctgag cttctgccgt agctttattg cgactgaatt ctcgattctg cactgtaggc
      961 tcgagcttca ccaagtcaga aggagacacg ttctttgctc tgttcgagcg gtggtctgac
     1021 cgaaatggac atcggaccag acacaactgg ccggctcatg cacaaggaat gcgatgcagc
     1081 gagaacatcc gacttgtggt cataaacgtc caaagaaaat ttgtttcgct cggtgaggaa
     1141 atgtctgagg ttccggcagt gcggacgggg cagcaggcga caaccctcgc actgctacca
     1201 ctcagtgatt catctgcttt cttagcagga ctccgtaaac tttggccgca aggtgactta
     1261 actgaagtgg cttagcgacg aactcagcct cacggtcgag aatcaccttt cctgcctctt
     1321 ggcccatata tgccgacatg atgatcacac cgatggctgg ccacctcgag cgaatctccc
     1381 gcaccaacgc caggccatgg attttcggca agacaaaatc tgagatgaca agatccacgc
     1441 tatcttcatt cagccggttt aacgcttcga gcccatcgct agcttcgaag acagaatatc
     1501 cctgtgattg gagaaatcgt gagacatttt tgcgagtggc atggtggtcc tcaacaatca
     1561 ggattttctc gcccatcggt taaagtccct tcaattctcc ccctgtcatc cctctgccaa
     1621 cctacaccga tagctcgata ggtcaatata ggtgagaatt ttttcatagt tattgtgcgg
     1681 ctgagtcgcc gttcatatgc gccgacgttt agatagcagg catcatgcgc tcgaagggaa
     1741 cgatgggcca ggcaactact tcttggagcc gagcaaagtg agccagtggt ttccgttccg
     1801 gaagctcgat tccggtcgac tgcgccatcg tgttcacgtc caagttcgct tcagccggct
     1861 gtagcaccca ttggggatga tgaatctcgc caaaaaccac ccgatcggga tctagcgccg
     1921 caaaaaggca atacctctcg gtgagccaat gatcgagcgt ccccgcagtc gcccggtaaa
     1981 ccgctccgat aggtctgtag tcggcgcgga actccgcgcg cggggagttc ttgtgggtgc
     2041 gcacgctgtc ataccgaata gtatcctcgg ctgatttgac tgttatgcgt gcatcgtaat
     2101 acggcaagcc aagccatcgc gccactctga cggcgagcca actcgctgcg tcgaggctga
     2161 aaaaccatac gccgctcttg cctgctgttt tcacataggt tcggacgttt agttccggca
     2221 atgccaacgg cagcggaaca taccgaggcc gaacgccgct catgtggaac ggaacaattc
     2281 cgatccagca ccatccatcg aaggtatcga gttcgagcgc catggggatc agaggcctga
     2341 tgtattcagg cttaatcggc caatggagaa acagcagatc atgccagcgc atgaacatca
     2401 cccagcggga ttttggcaga ggccaaggcc ggtgtgatgt ttggtccgtc accggcggca
     2461 cgcgcagact gcaacccaaa ctcactgtaa ccgccccggc tgcgatcgcg ctggattcga
     2521 tgaagttccg tcgattcata gctcgacctt atcgcagccg tttcgagctg acgctggatt
     2581 gcatggtgaa tcttcactcg cgcgcataag tcacaccggc actggcctcg atcacccgat
     2641 cagccaacca ctgcaacagg aatgatgtag aagttcgttg ccacgatacg tccgtcagac
     2701 cgtgatcggc gtatttgatc aagcggtgcg tcagcgatcg tgcccgggtg aaggcggcaa
     2761 ggtaattggc gatcaccgga tgcggcacta cgtcgtcctg ctcggactcg acgaccagca
     2821 catcgccgag aaacgccgcg cacgcgcgca gtgcccgatt ttcttctggc gccacaacac
     2881 gctttcgata agcgcgaaag tctggatctt catgtagctt gcgcttgggc aattcccaac
     2941 cggcgtcgtt atagagcgcc ggtgcgcgta gcgcgagcca ccctaccgcc cgctcggagg
     3001 tcaggatcgc cgcgagatac gcgccataac tgctgccgac cactcctaaa gatgctctgt
     3061 cgcagccagg ctggctggcc agtacatcgt atgccgcgag gagatctctt agattgtctt
     3121 cgcgtgacac taactcgcgt tgaccatcgc tacgcgcgtg accgcgcaga tcgaacgtca
     3181 aacagatgaa acccagctca gcgacggcgc gggcccgatt gaggtattgc tgttgattgc
     3241 cgccccagcc atggacaaac agcaccgcag ggccggcgct cacacccgcg atcagccgag
     3301 cctcgatctg ctgatcatca accgcgatct cgatcggctc ttcgtgaatt gtcatagact
     3361 ttgacttgag aatatttggt aatgggtccg acctgatcgt cgatcccttg gaaataaacc
     3421 accgcatccg gcggcactgg gtgctcgccg tacgactcga tagatgccgc gcgcaccgag
     3481 cgcaggctcg gatctgcacg aaaagcttcg agcgcggcaa tttccgcggc actggcgccg
     3541 ccgatacgcc aggattgctc gagcacgccg cacgagcggc gcccgtcatc atcgacgacg
     3601 tgcgcaacat cgtagttgcg ccgggaggct aaccatccag caaaggctcg tgaggcggcg
     3661 gcttcgtatg ttcgcgcgct ctttatcgcc actcgagccg ccggcggcag ctcgagactg
     3721 agcagatcat cgtaatcgcc gcgcacgaaa atcaaatctg agccgccgta aacgttggcg
     3781 ccttggttgt cttgggtcag taactgcctg ccataataag tcgccaagag gtccgcca
//
LOCUS       DASUTG010000594         8543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_686064, whole genome shotgun sequence.
ACCESSION   DASUTG010000594 DASUTG010000000
VERSION     DASUTG010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686064"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /gene="ilvN"
                     /locus_tag="VF089_13705"
     CDS             <1..227
                     /gene="ilvN"
                     /locus_tag="VF089_13705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6175067.1"
                     /translation="EAVRIADIFRAKIVDSSLTTYTIEITGDVDKVEALINMLKPLGI
                     KELIRTGRIGIARETTRTASPRREPALLKS"
     gene            401..1417
                     /gene="ilvC"
                     /locus_tag="VF089_13710"
     CDS             401..1417
                     /gene="ilvC"
                     /locus_tag="VF089_13710"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6175068.1"
                     /translation="MQVYYDRDADLKHLSGKKVAVLGYGSQGHAHANNLRDSGVDVVV
                     GLRRDSGSWEKAEKAGLKVLETAEAAKVGDIIMILLPDELQGDAYEKEIKPGLKNGNY
                     LAFGHGFNIHFKRVIPPAGCNVFMVAPKGPGHLVRSEFEKGRGVPCLLAVHQDPSGDT
                     TQVGLAYASALGGARAAVIQTTFKDETETDLFGEQAVLCGGLTALIQAGYETLVDAGY
                     PPEMAYFECVHEVKLIVDLIYEGGMSNMRYSISNTAEYGDLTRGKRVIGPEVAQAMKQ
                     ILGDIQSGKFAEEWINEYKCGMPHFSELRKEAGNHPLEKVGANLRSMMPWLAQNRLVD
                     KSKN"
     gene            1482..2111
                     /locus_tag="VF089_13715"
     CDS             1482..2111
                     /locus_tag="VF089_13715"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEX6175069.1"
                     /translation="MRVAKEGYPIILFTASLSAAVWAIGWSVVAGVLAILALAVLAFF
                     RDPERCPPDGEGLIVAPADGKVVSVTKVKQGQLMKKADSRVSIFLSPLDVHINRSPIG
                     GKVDDVKHTRGKFIAAYKDEASERNEQNSLRLLDDRDREVEVVQVAGVVARRIVCRAR
                     KSDFLSRGARFGLIMFGSRTDLYLPTSTRIEVAEGQRVKGGETIIGRFI"
     gene            2108..2962
                     /gene="pssA"
                     /locus_tag="VF089_13720"
     CDS             2108..2962
                     /gene="pssA"
                     /locus_tag="VF089_13720"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX6175070.1"
                     /translation="MNGINSPEPQPNPSAKVRLLHRKTRPRVPLRQRMPAEKLKKGVF
                     LIPSLFTAGNIMCGFFSIVSTFNGEYVQAVFFIILANILDCVDGYAARLTHTTSQFGV
                     ELDSLADVVSFGVAPAILVYFWALVPWGNWGWLAACTYVVCGALRLARYNVQAKSAAK
                     GHFVGLPIPAAAEMIAATVLIYYYLGGEGAANKHLTLLLVIYGLALLMVSGFHYLSVK
                     STDLRKRLPISALVFGLVLIKLAVLAPQVMFFTSFLLYTLSGPLLWCWTLLRRQRGKR
                     GETAKAIP"
     gene            3058..4623
                     /locus_tag="VF089_13725"
     CDS             3058..4623
                     /locus_tag="VF089_13725"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6175071.1"
                     /translation="MSDSIHNSHVKIFDTTLRDGEQSPGASMTVEQKVVIARQLEKLG
                     VDVIEAGFAASSDGDFESVRRVCKEVKRPRVLSLARSQDGDIERALRAVEHAANPGIH
                     IFIATSDIHLEHKLRMSREQVLEAAVKAVSNAKKHIDYIEFSAEDASRSALEFMIQIF
                     KEVIRAGANTINVPDTTGYAIPSEFGALMSNLIERTAGADRVIWSAHCHNDLGLAVAN
                     SLAAVHNGARQIECTINGIGERAGNTSLEEVVMALRTRKNYLDLDTNIITEQLYPTSR
                     LVSQVTGIPIPINKPIVGENAFAHEAGIHQDGVLKHKQTYEIMTPESVGISGNRLVMG
                     KHSGRHAFDERLKHLGFNLNKEDMNRAFLRFKDLADKKKNVYDEDIEAIIAEEILRVP
                     GQPDCYELLYLSVNSSSDGIPSATVKMRVDGQERMDHATGDGAVDACYKAITKITGSE
                     AQLVRYTVNAITGGADAQGEVSCQIQDRGIRVSGQGSHTDIIMASAAAYINALNKLQG
                     RKQYRQLVEREGP"
     gene            4620..5711
                     /gene="leuB"
                     /locus_tag="VF089_13730"
     CDS             4620..5711
                     /gene="leuB"
                     /locus_tag="VF089_13730"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX6175072.1"
                     /translation="MNYNITVLPGDGIGPEVMKQGTEVLQQVAQLYGFGVELQEGIVG
                     GASIDIHGQPLTDGVLNLCKQSDAVLLGAMGGPKWDGLDYSIRPERALLGLRQELRLF
                     ANLRPVKLFSALAAASTLKQEVVEGTDLLVVRELTGGIYFGQPKGVTKLADGTERGVN
                     TEVYTTPEIERIAHVAFQAALKRRKKVTSVDKANVLEATELWRKVVTRVHADEGYAAI
                     GLEHMLVDNCAMQLIRNPKQFDVVVTTNMFGDILSDEAAMLTGSIGMLPSASIGGKVG
                     MYEPVHGSAPDITGQDKANPLATILSVAMMLRHSLEQSAAADRIEKAVEDVLNGGYRT
                     ADIQEKDCRLVGCKEMGQLVRDRIAATKG"
     gene            5718..6740
                     /locus_tag="VF089_13735"
     CDS             5718..6740
                     /locus_tag="VF089_13735"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6175073.1"
                     /translation="MKKPKYNIAIVGATGAVGEQMREVLEERKFPVGELRLLASERST
                     GRFLEFEGRQHRVDVLHENSFEDIDIALFSAGGAVSAKYAPLAVSAGAVVVDNTACFR
                     MAPDIPLVVPEVNASAIRDYTNRRIIANPNCSTIQMVVALKPIHDAARIKRVVVSTYQ
                     SVSGAGRQAMEELSQQVAALYNSKEVEKKKFPHQIAFNCIPHIDVFMEDGYTKEEWKM
                     IHETRKILGEPSLPVTATTVRVPVFFSHSESVNVETEKKLTAAEVKALLREAPGIIVA
                     DEPENNVYPMALDATGKDATYVGRIREDHSVQNGINLWVVADNLRKGAALNAVQIAEI
                     LIRDYL"
     gene            6784..7515
                     /gene="truA"
                     /locus_tag="VF089_13740"
     CDS             6784..7515
                     /gene="truA"
                     /locus_tag="VF089_13740"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6175074.1"
                     /translation="MNIKLTVEYDGTDYCGWQIQPTGKTVQGVLEKAISTLLGRATRI
                     TGSGRTDAGVHALGQVANFLSDKKLEPYRVQRALNALTPEDVTIKTVEIVPDAFDARR
                     QGRSRIYEYRILNRSSPSPFHLRYAWHVHDSLDLAAMRQAITCLNGEHDFSSFRGAGC
                     DAAHPVRTVYATSINRTGDVIVFTVEATAFLRHMMRNLIGTLVEVGRQQRSPQSFAGL
                     LDACDRTQAGMKAPAQGLFLMEVKY"
     gene            7688..>8543
                     /locus_tag="VF089_13745"
     CDS             7688..>8543
                     /locus_tag="VF089_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6175075.1"
                     /translation="MMPAHNNFYDLYSKAKKIKIKWPNNARIAVALAGNLEAWTETPS
                     AKYRRTRHVGGSNPIKEEDVNSQYDCRTASENDYGGRTRVWRILRILDKHGVKASFNT
                     NALCIYRYPEAVKAVQARGHEIVGHSYAEDVQLIHLTEEEEREEVRTCAKMFQDFVGQ
                     KPTGWLTSGMRHTERTLSILANEGFLWHGDAVNDDNPYPVDINGKTLIVVPYKNAISG
                     LNDTGMYRRGITARDVFNSFKDEFDILYEESLEDPKMLTLAMHCQMAFPATGKVYDEA
                     IQYAKSHAD"
BASE COUNT         2061 a         2141 c         2425 g         1916 t
ORIGIN      
        1 atgaagccgt acgcattgcg gacatcttcc gggccaaaat tgtcgactca tcacttacca
       61 catataccat tgaaatcacc ggtgatgtcg acaaggttga agcgctgatc aatatgctaa
      121 agccattagg aatcaaagag cttattcgca cggggcggat cggcattgcg cgagagacca
      181 cgcggaccgc ttcacctcgt cgcgagccgg cgttgctcaa atcgtaaaac cgcggtcaga
      241 ttctgtgtca gaaaaccgtg gctagaacct cccgcgagat caggtaaagc gcttttcatc
      301 atccttttcg tttgtggcaa catccgtggt acgtctgcgc taattgacat aatcgtctgt
      361 tctggccata ttggattttc gctctaaaaa ggggaactta atgcaagtct attatgatcg
      421 cgacgccgac ctgaagcact tgtcgggaaa aaaggtagct gttttgggat acggcagcca
      481 aggccatgct catgcgaaca atttgcggga ttccggtgtg gatgttgtgg tcggtttgag
      541 acgcgacagt ggttcctggg agaaagccga gaaggccggt ctgaaggtgc tagaaacagc
      601 ggaggccgct aaagtcggcg acatcataat gatcttgctc cctgacgagc tgcaagggga
      661 tgcctatgaa aaggaaatca agccaggtct aaagaacgga aattacctcg ccttcggaca
      721 cggcttcaat atccacttca aacgcgtgat tccacctgca gggtgtaatg ttttcatggt
      781 cgcgcccaaa gggccgggac atctagtgcg tagcgagttt gagaagggac gcggcgtacc
      841 gtgcttgctt gccgttcatc aggatccgtc cggcgatacg acccaagtgg gacttgccta
      901 tgccagcgcg ctcggaggtg cccgcgcggc cgtcattcag accacgttta aagatgagac
      961 ggaaaccgac ctttttggcg agcaggcggt tctctgcggc ggattgacag cgttaattca
     1021 agcaggctac gagacgcttg tagacgccgg gtatccaccc gagatggctt atttcgagtg
     1081 tgtccacgaa gttaagctga tcgtcgacct gatttatgaa ggtggcatga gcaacatgcg
     1141 ctattcaatc agcaatacgg ctgagtatgg cgatctgacc cgtggaaaac gagtgatcgg
     1201 tcccgaggtc gcgcaggcta tgaagcagat tctcggtgat atccagtcag gcaaattcgc
     1261 agaggaatgg atcaacgagt ataagtgcgg catgccgcac ttctcggagc tgcgcaaaga
     1321 agccgggaac catccgctcg agaaagtggg cgcgaatctg agatccatga tgccttggtt
     1381 ggcgcagaat cgattagtgg acaagagcaa gaactgagcg ttgtaatcga tcgcatcgag
     1441 tcattgggtg gacggcgccg caccatgttg gatcatttag catgagggtt gccaaagaag
     1501 gttatccgat cattctgttc acggcgtcgc taagcgccgc agtatgggcg attggctggt
     1561 ccgtggttgc gggcgtctta gctattctcg cactagcagt gttggctttt ttccgggatc
     1621 cggagcgctg tcccccggat ggagaagggc tgatcgtcgc accggcagat ggtaaagtcg
     1681 tcagtgtcac caaagttaaa cagggccagt tgatgaaaaa ggcggactcg cgtgttagta
     1741 tttttttgtc gccactggat gtccatatca atcgttcgcc tatcggcggc aaagttgatg
     1801 acgtgaagca tacgcggggc aagtttatcg ccgcttataa ggacgaagct tcagagagaa
     1861 acgagcagaa tagcttgagg ctgctcgatg acagagatcg agaagttgag gttgtgcagg
     1921 ttgccggagt tgtggctcgg cggattgtct gccgcgctcg caaaagtgat ttcctaagca
     1981 ggggcgctag attcggcctg attatgttcg ggtctcgaac cgatctttac ttgccgacca
     2041 gcactcgaat cgaagtggcg gaagggcaga gggtaaaagg cggagaaaca atcataggac
     2101 ggttcatatg aacggaatca attctccaga gccgcaacca aacccctcgg caaaggtgcg
     2161 tctgctgcat cggaaaacac gcccccgtgt tccgctgcgc cagcgaatgc ctgcggagaa
     2221 actcaaaaag ggggtatttt taatccccag cctcttcacc gctggcaaca ttatgtgcgg
     2281 ctttttttct atcgtatcca ccttcaatgg agaatacgtt caggcggtgt tcttcattat
     2341 actggcaaat atactcgact gcgtagacgg ctacgcggcg cgcctgaccc ataccaccag
     2401 ccaatttggt gtagagctcg attctcttgc ggatgtcgtt tcctttggcg tagcgccggc
     2461 gatcctggtt tatttctggg cgcttgttcc gtggggcaac tggggatggt tggcagcttg
     2521 cacgtacgtc gtctgcggcg cattgcggtt ggcgcgctac aacgtccaag ccaaaagtgc
     2581 ggccaaaggc cattttgtcg gattgcccat tcccgccgcc gccgaaatga ttgcagcgac
     2641 agttctcatt tattactact tgggcggcga aggcgcggcc aacaagcatc tgacgcttct
     2701 tttggtgatc tacggtctgg ctctcttgat ggttagcggt tttcattatc tcagtgtaaa
     2761 gagcacggat ttgcgcaagc gtctgccgat atcggccttg gtttttggtc tggttttgat
     2821 taaactcgcc gttctggcac cgcaagttat gttttttacc agcttcctgt tgtatacgtt
     2881 atccggtccc ctcttatggt gttggacgct gttaaggcgt caacggggga aaagaggaga
     2941 gacggcgaaa gcaataccgt agccgcctct gttctgacac gcgaaaggac actcccgttg
     3001 gcactgccgc gggagttttt gtttctggac agtgttgacc gcagactggg aggatccatg
     3061 tcagacagta tacataatag tcacgtcaaa attttcgaca ccacattgcg cgatggcgag
     3121 caatcacccg gtgccagcat gaccgttgaa cagaaagtgg tcatcgcacg ccagttggaa
     3181 aagcttggcg tcgatgtcat cgaggcaggg tttgccgcct catcagacgg cgatttcgaa
     3241 tccgtaagac gagtctgtaa agaggtgaaa cgaccccgcg tgctgagcct ggcgcgttcc
     3301 caagatggag atatcgagcg ggcgctcaga gccgtcgagc atgcggcaaa tcccggcatt
     3361 catatattta ttgcgacttc ggacattcat ctggagcaca agcttcggat gtcgcgcgag
     3421 caggttttgg aagccgctgt caaggcagtt agtaatgcca agaaacatat cgactacata
     3481 gaattctcgg ccgaagatgc atcgcgcagc gcactggagt ttatgatcca gatattcaaa
     3541 gaagtgattc gcgccggagc caataccata aacgttccag ataccaccgg ttatgcaatt
     3601 ccttcagaat tcggcgctct catgagcaat ttgatcgaac ggaccgcggg cgcagatcgg
     3661 gtgatttgga gcgcacattg tcataacgac ctcgggcttg ccgtggcgaa ctcgttggcc
     3721 gccgtgcaca acggcgccag acagatcgaa tgcacgatta atggcatcgg cgagcgcgcc
     3781 ggcaatacct cactagaaga ggttgtgatg gcgctgcgca cgagaaagaa ttatctcgat
     3841 ctcgatacca atatcatcac cgagcagctt tatccgacat cacgcctagt atcacaggtc
     3901 actggaattc cgattccgat caataaaccg attgtcggtg aaaacgcttt tgcacatgag
     3961 gccggtattc atcaggatgg agtgctcaag cacaagcaaa cctatgaaat tatgactccg
     4021 gagtccgtgg gcatcagcgg aaatcgcctg gtcatgggca aacattccgg ccgacacgct
     4081 ttcgatgaac ggttaaagca tctcggtttc aatctcaaca aggaagacat gaaccgagcc
     4141 ttcctccgct tcaaagactt ggcggacaag aaaaaaaacg tctatgacga agatattgag
     4201 gcgatcattg ccgaagagat tctcagggtt ccgggacagc cggactgtta tgagctgttg
     4261 tatctgagcg tcaattccag ttccgacggc attccatcgg ccactgtgaa gatgcgggtc
     4321 gacggccagg agcgaatgga tcacgccacc ggagacggcg ccgttgatgc ttgctataaa
     4381 gccatcacca agataaccgg gtctgaggcg caactggttc ggtacacggt aaacgcaatt
     4441 accggcgggg ccgatgctca gggcgaggtc tcctgtcaaa ttcaagaccg cggcattcga
     4501 gtatcgggac agggctctca taccgacatc atcatggcca gcgcagcggc ttacatcaac
     4561 gcgctgaaca agctgcaggg acgaaaacaa tatcgacaac tggtggagag agagggccca
     4621 tgaattacaa catcacagtt ttgccggggg atggcatcgg ccctgaggta atgaaacagg
     4681 gcacagaggt gcttcaacaa gtcgcgcagc tttatggatt cggtgtcgaa ttgcaggaag
     4741 ggattgtcgg cggcgcgtcc atcgatattc acggccaacc tctgaccgat ggtgtcttaa
     4801 atttatgtaa gcaaagcgat gccgtgcttc tcggcgccat gggcggaccc aaatgggacg
     4861 ggctcgatta ttccatccgc ccggagcgcg cgctgcttgg cctacggcag gaacttagat
     4921 tgtttgccaa tcttcggccg gttaaattat tttctgctct ggctgcggcg tcgacgttga
     4981 aacaggaagt ggtcgaggga actgacctgc tcgtggtccg tgaattgact gggggaattt
     5041 acttcgggca gccgaaaggc gtgacgaaac tagccgatgg gaccgagcgc ggagtcaata
     5101 cagaggtcta taccactcct gaaatcgagc gtatcgctca cgtcgcattt caggccgcct
     5161 tgaagcgacg taagaaggtt acctcggtcg acaaggccaa cgtgctagag gcgaccgagt
     5221 tgtggcgcaa agtggtcact cgagtgcatg cggatgaggg ttatgcggcc attggactgg
     5281 aacatatgct ggtcgacaac tgcgccatgc aactgatccg caatcccaag caattcgacg
     5341 tggtcgtgac tacgaatatg ttcggagaca tattgagcga tgaagccgcc atgctgacag
     5401 gttcgattgg tatgctgcct tcagccagca tcggcgggaa agtgggcatg tacgagccgg
     5461 ttcatggcag cgcccccgat attaccgggc aagacaaggc gaatcctctg gcgaccattc
     5521 tcagcgtggc gatgatgctg cgtcattctc tcgagcagag cgcggcagcg gatcgcatcg
     5581 agaaagccgt agaagatgta ttgaatggtg gataccgaac tgcggatatt caagaaaaag
     5641 attgccggct ggtcgggtgc aaagaaatgg gccagttggt tcgtgacagg atcgctgcga
     5701 ccaagggcta ggcgaaggtg aaaaaaccaa agtacaatat cgctatcgtt ggcgccacgg
     5761 gagccgtcgg tgaacaaatg cgcgaagtcc tggaagaacg gaaattcccg gtgggggagc
     5821 tgcgcttgct cgcctctgaa cggtccaccg gacgatttct tgagtttgaa gggcgccagc
     5881 accgagtcga tgttttgcac gagaactcat ttgaagacat cgacatcgcc cttttttccg
     5941 ccggcggcgc tgtgagcgca aaatacgctc ctctagccgt atccgccggc gctgtggtgg
     6001 ttgacaatac tgcctgtttt cgcatggcgc cggatatccc actggtcgtc ccggaggtaa
     6061 atgcgagcgc cattcgcgat tacacaaacc gtcggatcat cgctaatcct aactgctcca
     6121 ccatccagat ggtggttgcg cttaaaccga ttcacgatgc cgcgcgcatt aaacgcgtgg
     6181 tggtctccac ctatcaatcg gtgtcgggcg caggcaggca agcgatggag gaattaagtc
     6241 agcaggttgc ggctctgtat aacagcaagg aggtcgagaa gaaaaagttt ccgcaccaaa
     6301 ttgcgtttaa ttgcatacct catatcgatg tttttatgga ggatggatac accaaggaag
     6361 aatggaagat gattcatgag acacggaaga tcctggggga gccgtcgctg ccggtgacgg
     6421 ccactaccgt gcgggttccg gtgtttttca gccattcgga gtcggtaaac gtcgagaccg
     6481 agaaaaagct caccgctgcc gaagtcaaag cattgctccg tgaagccccc gggattatcg
     6541 tggccgatga accggagaat aatgtttatc cgatggcact cgatgccacg ggcaaagatg
     6601 ccacctacgt aggccgcata cgcgaagatc actcggttca aaacgggatc aatctttggg
     6661 tggtggccga caacctgcgc aaaggcgcag cactgaacgc ggttcagatt gccgaaatct
     6721 tgattcgcga ctatctctga gcgttggcga tgatccgcca gtcacgtttc ctacgtcctc
     6781 tcagtgaaca ttaagcttac cgttgagtac gacggcactg attattgcgg ctggcagatt
     6841 cagccgaccg gcaaaacagt tcaaggggtt ttagagaagg ccatttccac gttattgggg
     6901 cgggctacac gtatcactgg ttcgggccga accgacgcgg gtgttcatgc gctcggacag
     6961 gtggccaact tcttatccga taagaaactc gagccttacc gcgtgcagcg cgcgctcaat
     7021 gcgctaacgc cggaagacgt cacgatcaag acagttgaaa tcgtgccaga tgccttcgac
     7081 gcccggcggc agggccgcag tcgcatctat gaatatcgga tattaaaccg ctcgagccct
     7141 tcaccgttcc atctgagata cgcctggcat gtgcacgaca gcctggactt agcagcaatg
     7201 cgtcaggcga tcacatgtct caacggagag cacgattttt cttccttccg tggcgctggt
     7261 tgcgatgccg cccatcctgt aagaacggtt tatgcaactt cgatcaaccg aaccggcgac
     7321 gtgattgttt ttaccgtgga agccacagct tttcttcgtc acatgatgcg caatctcatt
     7381 ggcaccttag tggaagtggg ccggcaacag cgctcacccc agtcctttgc cggacttctt
     7441 gatgcgtgtg accgtactca ggccggcatg aaagctccgg cgcaaggttt gttcctgatg
     7501 gaagtgaaat actagaagca gcgcccccaa gcggttgcct tgagggcggg ccataggtgc
     7561 cacaccacag gcatgctgcg cttcaggaga cttgctagtc gcaactttcg ttgacactgt
     7621 cggaaacttc tatatacctt agcccgatga gagtgactcc tgccacaatg aaggaggatg
     7681 acaaagcatg atgccggcac acaataactt ctatgacctt tactcgaagg ccaagaagat
     7741 taagatcaaa tggccaaaca acgcccgtat cgcggttgcg ttggccggta atttagaagc
     7801 ctggacagag actccaagtg caaaataccg gcgaacgcgg catgtcggcg ggtccaatcc
     7861 aatcaaggaa gaagatgtca actcacagta tgattgtcgt accgccagcg aaaacgatta
     7921 tggcggccgg accagagtct ggcgcatcct gcgcatcttg gataaacacg gcgtgaaagc
     7981 atcttttaat acaaatgcgc tttgcatcta tagatacccg gaggcggtaa aagcagtgca
     8041 ggcccgaggc catgaaattg ttggccactc gtatgccgag gatgttcaat tgatccatct
     8101 tacggaggaa gaggagcgcg aggaagtacg gacttgtgcc aagatgtttc aggattttgt
     8161 tggccagaaa ccaactggtt ggctgacttc cggtatgcgc cataccgagc gaactctgag
     8221 cattcttgcc aatgaaggtt ttctctggca tggggacgcc gttaatgacg acaatcctta
     8281 tccggtcgat atcaacggca aaacgttgat cgtggtgccg tacaagaacg cgattagcgg
     8341 actaaacgat actggcatgt atcggcgagg catcactgcc cgtgatgttt ttaattcatt
     8401 caaagatgag tttgacattc tttatgaaga gtccctcgaa gatcccaaga tgctgacctt
     8461 ggcgatgcat tgtcagatgg catttccggc gaccggcaag gtctacgacg aggcgattca
     8521 atatgccaag tctcacgctg acg
//
LOCUS       DASUTG010000595         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1723007, whole genome shotgun sequence.
ACCESSION   DASUTG010000595 DASUTG010000000
VERSION     DASUTG010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723007"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..977
                     /locus_tag="VF089_13750"
     CDS             51..977
                     /locus_tag="VF089_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003163886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175076.1"
                     /translation="MPPVISISSLTKTYASGYQALKGIDLEIRKGEIFALLGPNGAGK
                     TTLISIVCGIVTPTEGTVIVDGHDIVREYRAARSKIGLVPQELTTDAFESVWATVSFS
                     RGLFGHAADPVYIEKVLRDLSLWEKRKNKIMTLSGGMKRRVMIAKALSHEPEILFLDE
                     PTAGVDVELRRDMWALVRALQESGVTIILTTHYIDEAEEMADRIGVINKGGLIVVEEK
                     VELMKKLGKKQLTLTLLEPLTSLPLELSEWPVALKDGGRELEYTFHGNEESTGIPILL
                     KRLGGLGISFKDLNTRQSSLEDIFVSLVSDRK"
     gene            974..1762
                     /locus_tag="VF089_13755"
     CDS             974..1762
                     /locus_tag="VF089_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175077.1"
                     /translation="MSTSTKLSALNRHGVWAIYKFEMARALRTLLQSVVTPVMTTSLY
                     FVVFGAAIGSRMTEVNGVAYGSFIVPGLMMLSLLTQSISNASFGIYFPKFTGTIYELL
                     SAPVSSMEIVIAYVGAAATKSIVLGLIILATAALFVPITILHPVWMIAFLVLTAATFS
                     LFGFIIGLWAKGFEQLQFIPMLVVTPLTFLGGAFYSIDMLPPAWRTVTLFNPVVYLIS
                     GFRWSFYGTSDVSVGVSLAMTLGFFAICLAAVAWIFKTGYRLKS"
     gene            2010..2405
                     /locus_tag="VF089_13760"
     CDS             2010..2405
                     /locus_tag="VF089_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175078.1"
                     /translation="MKTFIVAFLIVFLGGILEPSGVRGNEFKEREAKISQYKKWLDSM
                     GSHGSKYWTRLDGRPRPHRLYLGEGFYRADFQSQERFVDTFSNYLAGHPEKFMLIDLF
                     DAATNKPVGEYGFGGFKLYTKPLQTAERP"
     gene            2665..>4349
                     /locus_tag="VF089_13765"
     CDS             2665..>4349
                     /locus_tag="VF089_13765"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX6175079.1"
                     /translation="MISAEQITQAFDSATRQFRQSDEYQELVAGGANIEVAREFLRNV
                     FRTHFLSSHIVALCFASLPSTAAEMLRENLLEEMGRSDDEKPHSALLLELAHGVGFSQ
                     SEIDGLIADARRRLAVFCATRVPASTLRELCLAVLLETMSFEYMLSRCSSEIAAALTN
                     HYGFAKPAFHWFALHSEVDVRHAQEGVTIIQDYLSFHQAPDDLFEQIASLTLGDNLFT
                     RHYFPAASKRRTRTRSAPANMRRIEAVTIYRLRIPFHQTFRHALHSREESDAVIVKVI
                     DSDGRSGFGESLPRSYVTGETSESMIARIREHLAPRIFAQSFAPGWETFEYLQSVASE
                     WTRADERQSGLSAWNAAFCAVELALLDWSLRVDHCALADLLRPARYEVVYSGVISADA
                     PNDAAVLAKRMAQLGIRQIKVKVGTADDAARLEAVRKAVGDEVALRADANGGWNGEEA
                     IEQLRRLARFKLQAIEQPVRADDLAGMKRVRQESGVAVMADESLVTFDQARRLIDLGA
                     CDYFNVRLSKNGGIGGSLAIARLAQEAGIKIQIGAQVGETGVLSAAGRILAAHL"
BASE COUNT          932 a         1238 c         1232 g          947 t
ORIGIN      
        1 aaaaggcgac gcgctggcta gtcgtcacgc aattgcgtta agatcaatcg atgccgccag
       61 tcatctccat ctcgagcttg accaaaacct acgcttccgg ctaccaggcg ctgaagggta
      121 tcgatctcga gatccgcaag ggtgaaatct ttgccctgct ggggcccaac ggcgccggca
      181 aaaccacttt aatcagcatc gtttgcggta tcgtcacccc aacggaaggc accgtgatcg
      241 tagacggcca cgacatcgtc cgcgaatacc gcgcggcgcg atcgaaaatc gggctggtgc
      301 cgcaggaact gaccaccgat gcgttcgagt cggtgtgggc caccgtgagc ttcagccggg
      361 gcctcttcgg tcatgcggcg gacccggttt acattgagaa agtgctgcgc gacttgtctt
      421 tatgggaaaa acgcaaaaat aagatcatga cgttgtcggg cggcatgaag cggcgcgtga
      481 tgatcgccaa ggctctatcg cacgagccgg agattctttt tctcgatgag ccgaccgctg
      541 gcgtggacgt tgagttgcgc cgcgacatgt gggcgctggt gcgagctctg caggaaagcg
      601 gcgtgaccat tatcctgacc acccactaca tcgacgaggc ggaggagatg gccgatcgga
      661 tcggggtgat caacaaagga ggactgatcg tggttgaaga gaaggtcgag ctgatgaaaa
      721 aacttggcaa gaagcagctg actttgactc tcctcgagcc tttgacatcg ctgccgctgg
      781 agctaagcga gtggccggtg gcgctgaagg atggcggccg cgagctggaa tacacttttc
      841 acggcaatga agaaagcacg ggaataccca ttttgctcaa gcggctgggt ggtctcggca
      901 tcagtttcaa ggacctgaac acgcggcaga gctcgctcga ggacatcttc gtcagcttgg
      961 tgagcgatcg caaatgagca cgtcaaccaa actatcggca ttgaacagac acggagtctg
     1021 ggcaatctat aaattcgaaa tggcgcgcgc cctgcgcacg ttgctgcaaa gcgtcgtaac
     1081 gccggtcatg accacttcgc tttatttcgt tgtcttcggc gccgctatcg gctcgcgcat
     1141 gaccgaagtg aacggcgttg cctacggctc cttcatcgtt ccgggattaa tgatgctgtc
     1201 gctgttgacc cagagcatct ccaacgcctc gttcggcatc tatttcccga agtttaccgg
     1261 gacaatctat gaactcttgt ccgctccggt atcctctatg gaaatcgtca tcgcttacgt
     1321 cggcgccgcg gcgaccaaat cgatcgttct cggcctgatc atcttggcga cggccgcgct
     1381 gttcgtgccg atcacgattt tgcacccagt atggatgatc gcgttcttgg tgctaacggc
     1441 ggctacgttt agtctcttcg gtttcatcat cggtctctgg gcgaaaggtt tcgagcagtt
     1501 gcagtttatc ccgatgctgg tggtaacgcc gctaaccttt ctcggcggcg ccttctattc
     1561 catcgacatg ctgccgccgg cctggcgcac ggtcacattg ttcaatcctg tagtctatct
     1621 catcagcgga tttcgctgga gcttttacgg cacctcggac gtgagtgtcg gtgtcagcct
     1681 ggcaatgacc ttggggttct ttgcgatctg cctggcggcg gtcgcatgga tatttaagac
     1741 cgggtatcga ctgaagagct gacgaaattg aaacgcactt tggtcgctct cgagcgataa
     1801 tgtgcgacaa gagattgcgc agcgactaaa tcgcggccaa tcgcaattcc gccactacag
     1861 cagattgccg cactcgagtt gccatcttgt cacaaaagag cgtgattgct cacgcgttcg
     1921 ccaagaggat tccacctatc gcgggatctg accgctcatg gcaaatgcct tgcgttagaa
     1981 accacccgac taattaatcg ggggaaatca tgaaaacgtt tatcgtcgct tttctcatag
     2041 tcttcttagg cggaattctt gaaccttccg gggtgcgcgg caacgagttc aaagaacgcg
     2101 aagcaaaaat cagtcaatac aaaaaatggc tcgattctat gggatctcac ggctctaaat
     2161 actggacccg tctagatggg cgaccgcgtc cccaccgatt atacctcggt gaaggcttct
     2221 atcgagccga ctttcaaagc caggagcggt tcgttgatac gttctcaaat tatctcgcgg
     2281 gacacccgga aaaatttatg cttatcgatc tcttcgatgc ggcaaccaac aaacccgtag
     2341 gcgagtatgg attcggcggc ttcaaacttt acacaaagcc tctgcagacc gcggagcgtc
     2401 cttaaagtag tgtaacagtc gctacgctcg atatatctgt accctgccgc cgaatccgct
     2461 acggtcgatc tcagtccaga ccaacaggcg gaatcttctc gaccctacgg aacgcgtcaa
     2521 gtatacttcc atagccgctc ctactcgaag caggcgagaa ttctctagat tagggagctc
     2581 ggtggatttg aagctgtaag cttttcccgc caaggcagtg agcttcgtgt ttaagcggca
     2641 taaccgttcc cgtactggaa gagaatgatt tctgccgagc aaatcaccca agcattcgac
     2701 agcgcgaccc ggcaattccg ccaatccgat gagtaccaag agcttgtcgc gggaggcgct
     2761 aacatcgagg ttgcgcgtga gtttctgcgc aacgtttttc gcacgcattt cctctcctct
     2821 catatcgttg ccctatgctt cgcatcattg ccctcgaccg cggcagaaat gctcagagag
     2881 aatctcctgg aggaaatggg gcgctccgat gacgaaaagc cccattccgc cctgctcctg
     2941 gaattggcgc acggcgttgg tttcagccag agcgaaatcg acggactgat cgccgacgcg
     3001 cgccggcgcc tcgcggtctt ctgcgccact cgagtgccgg cttcaaccct gcgcgagctt
     3061 tgcctggcgg tgctgctcga aaccatgagc tttgagtaca tgctgtcgcg ctgttcgagc
     3121 gagatcgccg ccgccttgac caaccattat ggctttgcca aaccggcctt ccactggttt
     3181 gcgcttcatt cggaagtaga cgtgcgccac gcacaagaag gggtgacgat catccaggac
     3241 tatctgagct ttcaccaggc acccgacgat cttttcgaac agatcgcgag tcttacgctc
     3301 ggcgacaacc ttttcacacg ccactatttt ccagccgcat cgaaacggcg gacccgcact
     3361 cgatcggcgc cggcgaacat gcggcgtatc gaagccgtga ccatttaccg gctgcgcatc
     3421 ccgtttcatc aaacgttccg tcatgcgctc cacagccgcg aagagagcga tgcggtgatc
     3481 gttaaagtca tcgatagcga cggccgcagc ggtttcggcg agagcttgcc gcgatcctat
     3541 gtcaccggcg agacaagcga atccatgatc gctcggattc gcgagcacct ggcgccgcgg
     3601 atttttgcgc aatcgttcgc tcctggctgg gaaacgttcg agtatcttca atccgtcgcg
     3661 tccgaatgga ccagagccga cgagcggcaa agcggcctct cggcgtggaa cgccgccttt
     3721 tgcgccgtcg aattggcatt gctcgactgg agcctgcgag tcgatcactg cgctttggcc
     3781 gacttgctgc gcccggcccg ctatgaagtt gtctacagcg gcgtaatctc ggccgatgcg
     3841 ccaaacgatg cggccgttct cgccaagaga atggcccagc tcggcatacg ccagatcaaa
     3901 gtgaaggtcg gcaccgctga tgacgcggca cggctcgaag cggtgcgtaa agccgtcggt
     3961 gacgaggtcg cattgcgcgc cgatgccaac ggcggctgga acggcgagga ggcgattgaa
     4021 caactacgcc ggttggcgcg cttcaagttg caggcaatcg aacagccggt gcgcgccgat
     4081 gatctggccg gtatgaaacg agtgcgtcag gagagcggcg ttgcagtcat ggccgatgag
     4141 tcgctggtca cgttcgatca ggcgcgccgg ctaatagatc tcggcgcttg cgattatttc
     4201 aatgttagat tgtccaagaa cggcggcatc ggcggcagcc tagcgatcgc cagactggcg
     4261 caagaagcgg gaatcaagat tcaaatcggc gcccaggtcg gcgaaaccgg cgttttgtcc
     4321 gccgccggac gaatcttggc ggcgcatct
//
LOCUS       DASUTG010000596         2307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_388982, whole genome shotgun sequence.
ACCESSION   DASUTG010000596 DASUTG010000000
VERSION     DASUTG010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388982"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1058)
                     /locus_tag="VF089_13770"
     CDS             complement(75..1058)
                     /locus_tag="VF089_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175080.1"
                     /translation="MYNGQTVVDVHGHMTTPAGFRQFLAEIVSQNTPGRKLEVTDEQL
                     ENAQQRHLKFMDDRNVDVQLIGPRPIAMWHWMRPFLQEFWAETTNNVIARIVKLYPDR
                     FRGMAQLPQCSTSKDTRNCLPEFERCVKELGFVGAYLNPDPGGDKQTPGVHEEYWFPL
                     YEQAVELDVPLMIHPSVTYDRRLEIIPANYQMNNYIEEFIAMQLLTHSKVFEIFPKLK
                     ITICHCGGGLNRFIPTDKHVGQGDFSNNLFYDSCVYDVHYLEAAIKQRGVDQILFGTE
                     SPGSGGAIRPETGMPSDDLIPVIDGINFLSTEDKLKIFQTNPLKVFTKVKI"
     gene            complement(1301..1783)
                     /locus_tag="VF089_13775"
     CDS             complement(1301..1783)
                     /locus_tag="VF089_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6175081.1"
                     /translation="MKSGIDSKGENIVRGIRRILYATDYSKASGRAREKAIELAKDYD
                     AELWVVHVIAPTTAYLTEQEFGGADLYMRLEESAKQQAESSMKRLMATLEKAKVKAKS
                     LLLRGSAHDQIIKAAKSKKADMIVIGTHGRTGLSKLFMGSVAGRVVSTATCPVLTVRQ
                     "
     gene            complement(1780..>2307)
                     /locus_tag="VF089_13780"
     CDS             complement(1780..>2307)
                     /locus_tag="VF089_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175082.1"
                     /translation="NRTGVERYRGSVNLKLMDIANALFELIFYGVLERYPRLKIVSVE
                     NEAGWLPWITQQWDYYYRRFRNANPPPFSKDPSEYFRNQIYSCFFNDSVCGHNLEWWG
                     QDNIMWSNDFPHPNSTWPNSLKIIQRDLGHLPVEVQTKVLATNVCKLYNLDMSKVPTG
                     IAKNALASQAVQASA"
BASE COUNT          511 a          592 c          624 g          580 t
ORIGIN      
        1 accgcaacgt cggtgaactc aagctcgatc acattcgcca acctgcattc agtctactac
       61 taattcacga tgacctagat ctttactttg gtaaaaacct tcagcggatt cgtctgaaag
      121 attttcagct tgtcttcagt actcaaaaag ttgatgccat cgatgactgg aattagatcg
      181 tcggatggca tgccggtttc cgggcgaata gcgccgccgg aaccgggaga ttcggtgcca
      241 aaaagaatct gatcgacgcc gcgttgttta attgcggctt cgagatagtg aacatcgtag
      301 acacaggagt cgtagaacaa attattactg aaatctccct gtccaacgtg cttgtcggtt
      361 gggataaaac ggttcaggcc accaccgcag tgacaaatag tgatttttaa ttttgggaag
      421 atctcaaata ccttgctgtg ggttagtagc tgcatggcga tgaactcttc tatgtaattg
      481 ttcatctggt agttcgccgg gatgatttcc agtcttcggt cgtaggttac cgatggatga
      541 atcatcagcg gtacgtccaa ctcgacggcc tgctcgtaca acggaaacca atactcctcg
      601 tgcacacccg gagtttgttt gtcgccacct ggatcggggt ttaaatatgc cccgacgaag
      661 cccagttctt tcacgcaacg ctcgaattca ggcaaacagt tgcgcgtgtc cttgctggtc
      721 gagcactgtg gcagctgcgc catgccgcga aaccggtcgg ggtagagctt gacgatacgc
      781 gcaatcacat tgttggtggt ttcggcccag aattcctgta ggaatggtcg catccagtgc
      841 cacatggcga tcggtctcgg tccaatgagc tgaacgtcga cattgcggtc atccatgaat
      901 ttaagatgac gctgctgcgc attctccagc tgttcgtcag tgacttccag tttccgcccg
      961 ggcgtgtttt gcgagacaat ttccgccaga aattgacgaa aacctgccgg ggtagtcatg
     1021 tgaccgtgaa cgtcgacgac ggtttgtccg ttgtacatta gaactcctca gagtcagtca
     1081 atggttcctt acatagcaga gcttaacccg gctctgtcaa gcgaggcgga ttggaattga
     1141 gcgagatgtg ctgtcaggag gtgaaccgcg ccgatcgagg tgatggatga aagctccaga
     1201 gggcttgctg cgcagcaggc agagaggctg tagcagttag gaaagtagcg gggagagagg
     1261 cgccgctctc acgctttcgg cgccttctct atgaagaatg ctattggcgg acggtgagca
     1321 cggggcaggt cgccgtcgag acgactcggc cggcgacgct gcccatgaat aatttggata
     1381 acccagtgcg gccatgcgtg ccgatcacga tcatatcggc ttttttgctt ttagccgctt
     1441 tgatgatttg gtcatgcgcc gatcctctca gcagcaggct cttggctttg actttcgctt
     1501 tctccagtgt tgccatcagc cgcttcatcg aactctcggc ctgctgcttg gcgctctctt
     1561 ccagcctcat atagagatcg gcgccgccaa attcttgttc ggtaaggtaa gccgtggtcg
     1621 gcgcgataac atgaaccacc cagagttccg cgtcgtaatc cttggcaagc tcgatagcct
     1681 tttctctcgc gcgtcctgac gctttggaat aatcggttgc gtacaaaatc ctgcggatgc
     1741 ctctcacgat gttttcccct ttgctgtcga ttcctgattt caagccgaag cctgaaccgc
     1801 ctgcgatgcg agcgcgttct tggcaatgcc ggtcggcacc ttcgacatgt ccaggttgta
     1861 tagcttacag acgttggtcg cgagcacttt tgtttgaact tccaccggca ggtggccaag
     1921 atcacgttgg ataatcttca gcgaattagg ccaagtcgaa ttaggatggg ggaagtcgtt
     1981 ggaccacata atattgtcct ggccccacca ctcgagattg tgaccgcata ccgaatcgtt
     2041 gaagaagcag gaatagatct gattgcggaa gtattcgctc gggtccttgc tgaatggcgg
     2101 gggattagcg ttccggaagc gccggtagta ataatcccac tgctgtgtga tccagggcaa
     2161 ccaacccgct tcgttctcga cggaaacaat cttgagcctg ggatagcgtt ccagcacgcc
     2221 atagaaaatc agctcgaaaa gcgcgttggc gatgtccatc agtttcaaat taacgctgcc
     2281 gcggtaacgc tcgacaccag tgcggtt
//
LOCUS       DASUTG010000597         2833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1277102, whole genome shotgun sequence.
ACCESSION   DASUTG010000597 DASUTG010000000
VERSION     DASUTG010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277102"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF089_13785"
     CDS             complement(<1..523)
                     /locus_tag="VF089_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6175083.1"
                     /translation="MTSHFDKSAGPGLRTAKASAQPRVGFLGVGWIGRHRLQSIVESG
                     AVEIAAVADFDSAAASEIAAQTPGAVAAPSLDALLELDLDGLVIATPTALHAEQAIAA
                     LEKDTAVFCQKPLGRSADETAQVIRAARLANRLLAVDLSYRFVRGVQKIKQMIGAGEI
                     GPIYVADLVFHNAY"
     gene            complement(516..1118)
                     /locus_tag="VF089_13790"
     CDS             complement(516..1118)
                     /locus_tag="VF089_13790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6175084.1"
                     /translation="MTRLIFIRHAATDSVSSRLAGRDANVHLSASGRPQGQRLARSLK
                     QRGIARVYSSPQPRARETAEFLGELLGDSIKIAPQLDEIDYGEWTGRSFNDLRSIAQW
                     RDFNSLRSCTRIPGGELMIEVQARVLGLMESLCRGHPAMTVALVSHADVIRGALAHCL
                     GMPLDLVLRLEISPASISMVGMEQDGPRVLCINNTEEIGD"
     gene            complement(1115..1807)
                     /locus_tag="VF089_13795"
     CDS             complement(1115..1807)
                     /locus_tag="VF089_13795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6175085.1"
                     /translation="MMIFFDIDQTLIDQQKAEAAAACELLAVYHDSLGQSYSVVSFCR
                     AWDFLRDKHARAFFAGVITSREQRRMRVRDLFGRVGWELSDGEADDCFTLYERHYRRH
                     WTLFDDVLPCLHALSHHRIGIISNGSAEQQKRKLAQTGIAAFFTVTVVSQEVGAAKPH
                     PDIFRAACRIAGCPAEQSIYVGDRLHVDALASQAAGMRGVWLDRRRTQRPATVQVVSS
                     LTELCAELRNPP"
     gene            complement(1870..2415)
                     /locus_tag="VF089_13800"
     CDS             complement(1870..2415)
                     /locus_tag="VF089_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6175086.1"
                     /translation="MKTRLCLDLELKIRQCARQDLPQLEWFGLYTEHRQHIEDAFRRQ
                     QAGEVIMLVADLDGFPVGQAWLDLSAREADSVGVIWALRVFPVLRNHGIGTHLMIAAE
                     QLLTERGFRWVELTVDQHEPRPRRLYERLGYRSAGSTEGLLSYTTPEEKSVALNLQLW
                     ILRKPLRPPATEWEVSQTSRE"
     gene            2435..2815
                     /locus_tag="VF089_13805"
     CDS             2435..2815
                     /locus_tag="VF089_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175087.1"
                     /translation="MPEASACSGAILRTPLGSNPVPDPAAMMNFHRLRELRFAALRPV
                     DFSPEVLPAPERLRALARPALTAIPLATRVFHDRGASAAGFGPSMDFVVVGGGSGLAS
                     TGHESPSSVDFFLVDSFVAVPAEL"
BASE COUNT          545 a          886 c          820 g          582 t
ORIGIN      
        1 cataggcgtt atgaaagacg agatcggcga catagattgg cccgatctcc cctgcgccga
       61 tcatctgttt gatcttttga acgccccgga caaagcgata ggacaggtcg acagccaaga
      121 gccggttggc cagccgcgca gcacgaatga cctgcgccgt ctcatcggcg cttcgtccca
      181 gcggcttctg acagaaaacg gccgtgtcct tctccagggc ggcaatagct tgctcggcgt
      241 gtaaggccgt cggcgtggcg atcaccagac catcgagatc caattcgagc aaagcatcga
      301 gcgacggcgc cgctactgca cctggagttt gcgccgcaat ctcgctggcg gcagcgctat
      361 cgaaatcggc cactgcggcg atctccaccg cgccgctttc gacaatcgat tgcagacgat
      421 gacgaccgat ccagccgaca ccgagaaatc ctactcgcgg ttgcgccgac gccttggcgg
      481 tgcgcaggcc aggtccggcc gatttgtcga agtgactagt caccgatttc ttccgtgttg
      541 ttgatacaga gaacccgcgg cccatcctgc tccatgccta ccatgctgat tgatgccgga
      601 ctgatctcca gccgaagcac aagatccagc ggcatgccca aacaatgggc caacgctccg
      661 cgaatcacat cggcatggct gaccagcgca acggtcatcg ccgggtggcc ccggcacagg
      721 ctctccatca gcccaagcac ccgagcttga acctcgatca tcagctcgcc gccagggata
      781 cgcgtgcagc tacgcagcga attaaaatcg cgccattggg cgatgctgcg caagtcgttg
      841 aaactccgcc cggtccattc gccatagtcg atctcgtcaa gttgcggagc gatctttatg
      901 ctgtcaccca gaagctctcc caagaactcc gcggtctcac gcgctctggg ttgggggctg
      961 ctgtagactc tagcgatgcc ccgttgcttc aaggaacgcg ccaagcgttg accttggggc
     1021 ctgccgctgg cgctcaaatg cacgttcgca tcccggccgg caaggcggct tgaaacggaa
     1081 tccgtggcag cgtgccggat aaagatcaac cgagtcatgg cgggttgcgt aactcggcac
     1141 aaagctcggt tagtgaactc accacctgca ctgttgccgg gcgctgcgtg cgccgtcgat
     1201 cgagccaaac gccgcgcatg cccgctgcct ggctcgccag cgcgtcgacg tgaaggcgat
     1261 cgccgacata gatggattgt tccgccgggc aaccggcaat gcggcaggcg gcgcgaaaga
     1321 tatcgggatg aggcttggcg gctcccactt cctgagacac caccgttacc gtaaagaaag
     1381 ccgctatgcc ggtttgcgcc agcttgcgtt tttgctgctc ggcactgccg ttggaaatga
     1441 tgccgatacg atgatgcgat agcgcgtgca ggcagggcag cacgtcgtca aacagagtcc
     1501 agtgccggcg atagtgcctc tcgtagagcg taaagcaatc atcggcttcg ccatcggaga
     1561 gctcccaacc gactcgaccg aacaggtcgc gcactcgcat ccggcgctgc tctcggctgg
     1621 ttatgacacc ggcgaagaac gcccgcgcat gcttgtcgcg caagaaatcc caagcccggc
     1681 agaagctgac caccgaataa ctttggccaa gtgaatcgtg atagaccgcc agcaactcac
     1741 acgccgccgc tgcttcggct ttttgctggt caatcagggt ctgatcgata tcgaagaaga
     1801 tcatcattgc tgtgcagcgt ttggagttgc tttggagtga gcctctggat gacgtatttt
     1861 cctgttcgac tattcgcggc ttgtttgaga aacctcccat tcggttgccg gcgggcgcag
     1921 tggcttgcgt aaaatccaga gctggaggtt cagcgccacc gatttttctt ccggtgtggt
     1981 gtagctgagc agcccttcgg tggagcccgc tgagcgataa cccaggcgct cgtaaagccg
     2041 gcgcgggcgc ggctcatgtt gatccaccgt cagctcgacc caacgaaaac ctcgttcggt
     2101 cagcaactgt tctgcggcga tcatcagatg ggtgccgatg ccgtgattgc gcagcactgg
     2161 gaacacccgc agggcccaga tcacgcccac tgaatccgct tctcgagcgc tcaaatcgag
     2221 ccacgcctgg ccgactggaa agccgtcgag atcagccacc agcattatta cttcgccggc
     2281 ttgctggcgt cgaaaagcat cctcgatgtg ttggcgatgt tcggtataga ggccgaacca
     2341 ctcgagttgc ggcaaatcct ggcgcgcgca ttggcgaatc ttcagctcca gatctaaaca
     2401 gaggcgcgtt ttcattcttc catcagtggg atgcatgcca gaagctagtg cctgcagcgg
     2461 cgctatcctt cgtacacccc tcgggtccaa cccggtgccg gatccagccg cgatgatgaa
     2521 ttttcaccgg cttcgcgagc tgcgcttcgc cgctttgcgg ccggtcgact ttagccccga
     2581 ggttttgccg gcgcccgaac gcttgcgcgc gctcgcccgg cctgcgctta cagccatccc
     2641 ccttgccacg cgtgtttttc acgaccgcgg ggcgtccgcc gcgggcttcg gtccatcgat
     2701 ggatttcgtc gtggtcggtg gtggttctgg attggcttct actggccatg agtcaccctc
     2761 ctcggtggat ttctttttgg tcgattcatt cgttgccgtt cctgccgagc tataacgatg
     2821 caccattcgc gcg
//
LOCUS       DASUTG010000598         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2019508, whole genome shotgun sequence.
ACCESSION   DASUTG010000598 DASUTG010000000
VERSION     DASUTG010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019508"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VF089_13810"
     CDS             complement(<1..1269)
                     /locus_tag="VF089_13810"
                     /inference="COORDINATES: protein motif:HMM:NF026141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX6175088.1"
                     /translation="MENVEIAHVLSKYADLIEIEGEDLFRVLAYRNAARTIESLSQPV
                     AQLLAEGKDLKKLKLPGIGKSMAEHIEEILKTGTLETLTQIQKQLPAKLHELLDIDGL
                     GPKRAKQLYELLGIGSLAQLEKALDSGKVESLRGFGQKSVQKIRQGIENLAKRPKRLK
                     LLEADQLVRPLIEYLRKGGNIEQLEVAGSYRRRVETVGDFDILVASEKPEPIMQRLQR
                     YPGVDRIIASGTTRGTVVLRSGLQVDLRILPRHCYGAALHYFTGSKAHNIAVRKLGVE
                     RGLRISEYGVFKVPKGKKAEYAGIEEGERIGGPREEDVFRAIGIDWVPPELREDRGEI
                     QAAQRHQLPKLIELDDIRGDLHLHSKWSDGKNSLLDMVRACKERGYEYCAITDHSKAV
                     RVAGGLTAQDLSQQRKEIEQAREKVAGITVF"
     gene            complement(1615..>3015)
                     /gene="ppsA"
                     /locus_tag="VF089_13815"
     CDS             complement(1615..>3015)
                     /gene="ppsA"
                     /locus_tag="VF089_13815"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX6175089.1"
                     /translation="SQKSLDKVEVYHLKEKGPALVRGRSVGEKIAHGPVRVVKNAHNL
                     NQVQKGDVLVTDKTDPDWEPTMRKAAAIVTNRGGRTCHAAIVSRELGLPAIVGTENAT
                     EVLKDGQAVTVSCAEGETGFVYQGLLKFDVQQIKLHDLSRPQTQVMMNVGNPEEAFRL
                     SSLPNDGVGLAREEFIISNYIKVHPLALLDYDRIEDQALREQIAQLTIGYDDKPEFFV
                     DKLAQGVAMIGAAFYPKDVVVRLSDFKTNEYANLIGGKAYEPVEENPMIGFRGASRYY
                     NPRYQAGFALECRAMKKVRDEMGLSNVKLMVPFCRTVEEGRRVLEEMEKHGLKRGDNK
                     LEVYVMCEIPSNVILANEFAEIFDGFSIGSNDLTQLVLGVDRDSEIVAHVFDERNPAV
                     KNMIASVIQAAKSKGRKIGICGQAPSDYPEFAAFLVELGIDSISLNPDAVLKTTVKIL
                     EIEAAQDSPRAIAASS"
BASE COUNT          593 a          882 c          803 g          737 t
ORIGIN      
        1 gaagaccgtg atgccggcga ctttctcccg agcctgctcg atctctttgc gttgttggct
       61 taaatcctgc gccgtaagcc caccggccac tcggaccgct ttactgtgat ccgtgatcgc
      121 gcagtactca tagccgcgct ctttgcaggc tcgcaccatg tctagaagcg aattctttcc
      181 gtcgctccac ttgctgtgca ggtggagatc tcctcgaata tcgtcgagtt cgatcagctt
      241 gggcagctgg tgcctctgcg cagcttgaat ctcgccgcga tcttcgcgca gctccggcgg
      301 cacccagtcg attccgatcg ctcggaagac atcttcctcg cgcgggccgc cgatccgctc
      361 gccttcttcg atcccggcgt attcggcctt cttccccttc ggcaccttga aaacgccgta
      421 ttcgctgatg cgcagcccgc gttcgacgcc taacttgcgc accgctatgt tgtgagcctt
      481 cgagccggta aaataatgaa gcgccgctcc gtaacaatgc cggggtaaaa tgcgcagatc
      541 cacctgcaag cccgagcgca gcaccacagt ccccctcgtt gtgccagagg cgataatgcg
      601 atccactccc ggatagcgct gcaagcgctg cattatcggc tccggttttt cgctggcgac
      661 caggatatca aagtcgccga ccgtctccac ccgacggcga tagcttccgg cgacttcgag
      721 ctgctcgatg tttccgcctt tgcggagata ctcaatcaac ggccgcacca gttgatcggc
      781 ctcaagcaac ttgaggcgtt tggggcgctt cgccagattc tcgatgccct ggcggatttt
      841 ttggacgctc ttctggccaa agccccgcag cgattctact tttcccgaat ctagcgcctt
      901 ctcgagttgg gcaagggagc cgatccctaa caattcgtag agttgcttgg ctcgtttggg
      961 cccgagtccg tcgatatcga gaagctcgtg gagcttggcg ggaagttgtt tctgaatctg
     1021 cgtcagagtt tcaagtgtgc cggtcttaag tatctcttcg atgtgctcgg ccatgctttt
     1081 accgatgccc ggcagtttca gtttcttgag atctttgcct tccgccaaca gttgagccac
     1141 cggttgggaa aggctttcga tggtgcgcgc ggcatttcgg taagcgagca cgcggaatag
     1201 atcctcgcct tcgatctcaa tcaggtctgc gtacttgcta agtacgtggg cgatctctac
     1261 gttttccatg gtcatcacat aaacgcgagg gctgctgaaa gtcgccacga gcgaaatgcg
     1321 cccacctctt tgccccctaa taacgcggcg gaggaagaag ggggctgccc cgtcgccgca
     1381 tcttgcacga gccagaattc ttggcccgat tccggtccga tacgttgcgt cagagaaagg
     1441 aatcgttctt cgacagtttg agaacagcgc cagtcataaa agcctcgtcc gacatttccc
     1501 gcctgtcttt cacgcaatag agaaaatctt catgggtagc ttgccgactg aagtcgctgc
     1561 ggcccaagtt ccatattcct tacccgctcc caaggggcag aaggtccaaa gcagctagct
     1621 ggatgccgcg atggctcgcg gagaatcctg agcagcttcg atttccagga ttttgacggt
     1681 tgtttttaga accgcatccg gattgagcga gatactgtcg atgcccagct caacaagaaa
     1741 tgcggcaaac tcaggataat cactgggcgc ctgcccgcag atgccgatct ttcttccttt
     1801 gctcttggcg gcttggatga cgctagcgat catgtttttc acggctggat tgcgctcgtc
     1861 gaaaacatgc gccacgatct ccgagtcgcg gtccactccg aggacgagct gggtgagatc
     1921 attagagccg atggagaagc catcgaagat ctcggcgaat tcgtttgcca gaatgacgtt
     1981 gctggggatt tcgcacatga catagacttc caacttgtta tcgcctcgtt ttaatccatg
     2041 cttttccatt tcttcgagga cgcgccgacc ttcttccacc gtgcggcaga aaggaaccat
     2101 gagcttcacg ttgctgagtc ccatctcgtc gcgcaccttt ttcatggcgc ggcattctaa
     2161 agcaaagccc gcctggtaac gcggattgta ataacgtgag gcaccgcgga agccgatcat
     2221 cgggttctcc tcgaccggct cataggcctt gccgccgatg agatttgcgt attcgttggt
     2281 cttgaagtcg ctcagacgca cgacgacatc tttcggataa aaagcggcgc cgatcatggc
     2341 cacgccttga gccagcttgt cgacaaaaaa ctcaggtttg tcgtcatagc cgatcgtgag
     2401 ttgcgcaatt tgctcgcgta gagcctggtc ttcgatgcgg tcgtagtcga ggagagccag
     2461 tgggtggacc ttgatgtaat tcgaaatgat aaattcttct cgagccaaac caacgccatc
     2521 gtttggaagc gaagagagac gaaaagcctc ctctggattg ccgacgttca tcatcacttg
     2581 agtctggggc cgtgacaaat cgtgaagttt gatttgttgc acatcaaatt tgagcaaacc
     2641 ctggtacacg aatccggtct ctccctcggc acaggaaacc gtgacggctt gcccgtcctt
     2701 taatacttcg gtggcgttct ccgtgccgac gattgccggc agtccaagct cgcggcttac
     2761 aatagcggcg tggcaggtcc ggccgccacg gttcgtgaca attgcagccg ccttcctcat
     2821 ggtcggctcc cagtccggat ccgtcttgtc ggtgaccaag acatcgccct tctgaacttg
     2881 gttgaggttg tgcgcgtttt tcactacgcg cacgggtcca tgagcgatct tttctcccac
     2941 gctgcggccg cgcactagcg ccggcccctt ttctttcaga tgataaacct cgactttgtc
     3001 caaagatttc tgcga
//
LOCUS       DASUTG010000599         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_686532, whole genome shotgun sequence.
ACCESSION   DASUTG010000599 DASUTG010000000
VERSION     DASUTG010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686532"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1021
                     /locus_tag="VF089_13820"
     CDS             110..1021
                     /locus_tag="VF089_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6175090.1"
                     /translation="MKTRVVSKLNGRPGNVNNLLLPLTSAGHVVNTRSMAFPPNVYVG
                     TSGWAYPDWLGSFYPAGSKPEDLLPHYARYFRAVEIDSTYSTIPARSVVAGWKENTPS
                     GFIFAAQVPGIITHQKVMKDCQRELTTFLNSLDLLGDRLGPLLLQFPYFNRNSFASRA
                     QFDRLLRPFLKTLPKEFKFAVEIRNKNWITWDLLEMLRDHSVAFVVVAQAWMPSIDVL
                     ARALDLLCADFFYARFIGDRREAQTQRSDHLTEDKTSELMVWIEDLKKIADRAPRTYA
                     FFSNHYAGFAPGSVKLFHELWHKAPQP"
     gene            1347..2876
                     /locus_tag="VF089_13825"
     CDS             1347..2876
                     /locus_tag="VF089_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6175091.1"
                     /translation="MPTQCVINLDLADVWDKRGRKSLRRTLAWGDEITVTDQKSTHLE
                     VEVTSFKTASDGSLIPSTRGGFIEPTKASRIKPADIIVPREQNQVLKVNFVDVQQGDG
                     SIIESPDGKIILVDGGDNQLFARYLAARFRGTSLNKPKRIDCVLITHGDADHFSGLLE
                     IHKSETHHNKLKRIFIHPERVYHNGLVKRPATKANKPVADRDLLGRTKQIGKKVIITG
                     LVDDLLRVADDELNQPFRRWKAALKEYSSRGAVSFRRLEFGDSEAFGFFNNGKLQIDV
                     LGPSITESGNVRGLRFFGVPLKVPRVGHASLELGEEGFTGLSAAHTINGHSVVFRLSY
                     GGFSFLFTGDLNDEASRFLAREHNRGTLNLRSEVFKVPHHGSADFSGGFFQAVSPVVN
                     IVSSGDENARKEYIHPRATLMGALGRWSRVPEPLVFVTELVAFFNVEGWSRLTDDKKA
                     KSRGDFFGFSRSAFGLVQTRTDGQRLLVYTDSGNVQMKEAYAYQLDESGVPIPAAVKR
                     G"
     gene            3451..>4700
                     /locus_tag="VF089_13830"
     CDS             3451..>4700
                     /locus_tag="VF089_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6175092.1"
                     /translation="MTTIAFESIVIIFLIIANGLFAMSELAIVAARKSRLQDWAEKGS
                     ASARSALELAENPNQFFSTVQIGITLVGILAGAFGGRTLASELSTYLAGIPAIEPYSS
                     TIALGAVVIGITYLSVVVGELVPKRIALSHAERIATFIAGPMRLLSAISSPIVRLLTA
                     STDAIFKLLGSSPTQEPPVTEEEIRRLIQQGTAAGVFEESEQDMIEAVIELGDKSARA
                     LMTPRTQIVWLDQTDSIEELRSRICESGHSRFPVGNGTLDNLVGVTQAKDLLTNALYG
                     KPIDLSASLQTPVLVPRTLTALQVLDYIKRSGSHLVLVVDEYGGIEGLLTHHDVLEAI
                     AGDMPFGETVTPPKAVQRGDGSWLLDGMIPVDEFKEIFDLDSLPGEKQDAYQTLGGFI
                     FTRMGRVPSVAESFEWNHLRFEIV"
BASE COUNT         1091 a         1274 c         1241 g         1094 t
ORIGIN      
        1 accgacgggc gaataaagcc gcgctcgtcc caatactgaa tctggcggag agaaacgccg
       61 gcgatccggc tcgcttcctt gctgttgtag gtttccatca aaagcgggaa tgaaaacaag
      121 agttgtttcc aagttaaacg gccgccctgg taatgtcaac aatttactgc ttccgttgac
      181 ctcggcagga catgtggtga acactagatc tatggctttt ccgccgaacg tttatgtggg
      241 aacctctggt tgggcatacc cggactggct cggctcgttt tatccggcgg gttcgaagcc
      301 cgaggacttg cttccacatt atgcgcgcta ttttcgcgcc gtcgagatcg attccacgta
      361 ctctaccatt ccagcgcgct cggtggtggc cggatggaag gaaaacactc catcagggtt
      421 tatttttgcg gcccaggtgc caggtatcat cacgcaccag aaagttatga aagattgcca
      481 gcgtgagctc acgacgtttt taaatagcct agaccttctc ggggaccgac ttgggccact
      541 gctcctgcaa tttccttact tcaaccgaaa tagttttgct tcgcgagcac agttcgacag
      601 gctgctgcga ccttttttga aaactctgcc aaaagaattc aagtttgctg tggaaatacg
      661 aaataaaaat tggatcacct gggatcttct cgagatgcta agagaccact ccgtggcttt
      721 cgtggttgtc gcccaggctt ggatgccgag catcgatgtc ctcgctcgcg cgctcgatct
      781 cctctgcgcc gacttctttt atgctcgttt tatcggcgac cgaagagaag cccaaaccca
      841 acgctcggac catctgaccg aagacaaaac gtctgaatta atggtgtgga ttgaagacct
      901 caagaaaatc gccgaccgag cgccgcgaac gtacgctttt ttcagcaatc actacgcggg
      961 ctttgcgccg gggtcggtaa agctttttca cgaattgtgg cacaaggcgc ctcaacctta
     1021 aagcagatat taagtgatgc gagtgcattg acatcagttc tgtcgagata ttccatgtca
     1081 aaccggaggc cgccgattgc aaagatcagg agtcaagcct ccggaacctt gaacagccct
     1141 gacttcatcg ttatttcaca gtgcagacct tttagatgac cgcgtttgtt ttcatatgac
     1201 gcatagacat cgtaatgtaa cggcgctcag gataactgtt caaccgttta accaggcgac
     1261 tagctaacgc ggctctggga tttaatcacc gatgagatcc actcaggcct catggtagcc
     1321 ggccggcgcg cctaaaggac cagacgatgc ctactcaatg tgtcatcaac ctcgacttgg
     1381 ctgatgtttg ggacaagcgc ggacgcaagt cgttgcgccg cacgctggcg tggggcgacg
     1441 agatcacggt gactgatcaa aaaagcactc atctggaagt tgaggtcacc agcttcaaga
     1501 ccgcttccga cggcagccta attccctcca cgcgaggcgg attcatcgaa ccgacaaaag
     1561 cgtcgcgcat caaacctgcc gacattatcg tgccgcggga acaaaatcag gtcctcaagg
     1621 taaacttcgt cgatgttcaa caaggcgacg gcagtatcat cgaatctccg gatggaaaaa
     1681 ttatcttggt ggacggcggt gacaaccaac tcttcgcccg ttacctggcg gcccggtttc
     1741 gcggcaccag cctcaataag cctaaacgca tcgattgcgt tctcatcacc cacggagacg
     1801 ccgaccattt ttcaggactg ctcgaaattc acaaatcaga gacccaccat aacaagctca
     1861 agcgaatctt cattcaccca gagcgtgtat accacaacgg gctggtgaag cggccggcca
     1921 caaaagctaa taaaccggtt gccgatcgag atctgctcgg tcgaaccaag caaatcggca
     1981 agaaagttat catcacaggg cttgtcgatg atttgctgcg ggtcgcggac gatgagttga
     2041 accagccgtt tcgccggtgg aaagccgcac tgaaagaata cagttcccgc ggcgctgtat
     2101 cgtttcgtcg gcttgaattc ggcgacagtg aagcattcgg cttcttcaat aatggcaagc
     2161 tgcagatcga cgttctcggc ccctccataa ccgagagcgg caacgtgcgc gggctgagat
     2221 ttttcggcgt cccgctcaaa gtaccgcgcg taggacatgc ctctctggaa ttgggagaag
     2281 aaggtttcac aggcttgtcg gcggcgcaca cgatcaacgg tcactccgtg gttttccgac
     2341 tctcttatgg cggctttagt ttcctgttta ccggtgatct aaacgacgaa gccagccgct
     2401 ttctcgcgcg cgagcataac cgcggcacgc tcaaccttcg atcggaagtt ttcaaagttc
     2461 cccatcacgg ttcagccgac ttctccggcg gtttcttcca agccgtatcg ccagtcgtga
     2521 atatcgtttc cagtggcgac gagaatgccc gcaaggaata catccacccg cgggcgacat
     2581 tgatgggagc cctcggacgt tggtcacggg tgccggaacc cctcgtgttt gtcaccgagc
     2641 ttgtggcatt cttcaatgta gaaggatggt cgcgactcac cgacgacaag aaagcaaagt
     2701 cccggggtga ctttttcggc tttagccgct ccgcgttcgg actagtgcaa actcgcactg
     2761 atggtcaacg tctattggtc tacaccgaca gcggcaacgt gcaaatgaag gaagcttatg
     2821 catatcagct cgatgaatcc ggcgtgccga ttcccgcggc agtgaagagg gggtaacgat
     2881 gccaaagcag ctttcgtttg acaagccacg ctggcggatg aatattgcgg catagcgcga
     2941 gggctcctgg cgctcctgat gatgatgata tcttggctcc ttgccggctt tgacgtctta
     3001 gaaaatccca acccaagtga actctcttcc cctgccgctc ggttagctga ccatggacaa
     3061 accgcgaagc ccagcggaga ttttgttcat tacccgaatc actccaaact ttactatttt
     3121 cgcttttgcg gagtagcgtc cggaccgcta ctgaccggct ctacggcacc ttggctaaag
     3181 ccagacaagc tttgcaactc cgccgtgctg tcttcgattg ctgggattgc gccgacagaa
     3241 ttcttaaaca gtcactcagg accaccttgc ctgaatagaa gattggcgat tttcgccctt
     3301 ccaatcgttc gagagagttg tcgattgagc gccgcttatc gaatccgtgc gagtcgcatt
     3361 caaaaacgca atgtgcagcc cttctatcac ttaggcccgg ctacgattca aaaggaacat
     3421 gctttcatac caaacgcagg atcgaactaa atgaccacca tcgcatttga atccatcgtg
     3481 ataatctttt tgattatcgc caacggactg ttcgcaatgt cggaacttgc aatcgtcgcc
     3541 gcgcgcaagt cacggcttca agattgggcg gaaaaaggca gcgccagcgc cagatcggct
     3601 ctagagctcg cggagaatcc gaaccaattc ttttccacgg ttcagatcgg catcacgctt
     3661 gtaggcattc tcgccggtgc cttcggcggg cggacactag cttcggaact ctccacctac
     3721 ttggcgggaa ttccagccat tgaaccgtac agcagcacga tcgcactcgg cgcggtggtc
     3781 atcggcatta cgtacttatc cgttgtggtc ggcgaattgg tgcccaagcg aatcgccttg
     3841 agccatgcgg agaggatcgc aacttttatc gcgggaccga tgcgactgtt gtcggccatc
     3901 tcctcgccga ttgttcggct tttgacagct tcaacggatg ccatcttcaa gttgcttgga
     3961 agttcgccca ctcaggagcc tccggtgact gaagaagaaa tcagaagatt gattcaacaa
     4021 ggaaccgcag cgggtgtttt cgaagagagc gagcaggata tgattgaggc ggttatcgag
     4081 ctgggcgata aaagcgcgcg cgcgctgatg acgccgcgca cccagatcgt ttggttggat
     4141 caaaccgatt ccattgagga gcttcgcagc aggatctgcg agagcgggca ttcccgattt
     4201 cccgtcggca acggaaccct tgataactta gttggcgtca cacaagccaa ggatcttttg
     4261 actaacgcgt tgtacggcaa gccgatagac ctctcggcgt ctttgcaaac cccggtactg
     4321 gtgccgcgga ctttgaccgc cttgcaggtg ctcgattata tcaaacggtc gggcagccac
     4381 ctggtgttgg tagtggacga gtatggcggc atcgaaggat tgctgacgca tcacgacgtc
     4441 ctggaagcca ttgctggcga tatgccgttt ggcgaaaccg tcacgccgcc taaggctgta
     4501 caacgtggcg atggctcatg gctgctggat ggaatgattc ctgtcgacga attcaaagaa
     4561 atttttgatc tcgacagctt gccaggagaa aagcaagacg cctaccaaac cttgggtggt
     4621 tttattttta ccaggatggg tcgcgttccg tcggtggcgg aatcttttga atggaatcat
     4681 ttgcggtttg agattgtcga
//
LOCUS       DASUTG010000600         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_241551, whole genome shotgun sequence.
ACCESSION   DASUTG010000600 DASUTG010000000
VERSION     DASUTG010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241551"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VF089_13835"
     CDS             <1..1013
                     /locus_tag="VF089_13835"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175093.1"
                     /translation="SHKRFATVSLIGLSLLFLLSNQLFAQPVDQEAAKKEGRIVVYGT
                     TIPKVMAPIHAGFEKRYGVKVDYWRASATAVTDRAITEWRAGKPGFDVVFAINGTITL
                     LKRENALAKFTPPAAAKFPAHLKDKDGVLTAFRHTPVSILYNTELVKPADLPKGFDDL
                     LDPKWRGKIAMPDPSRHTSTAQFLWNMHKVKGEKWLDYARALAKQKPFLLESFAPVPT
                     ALVKGEAQIGITYAQYITQVKGPLSHVVFDKVLTDSTDLALSAKAAAPNAAKLYIDYL
                     CTADVQKIIADSGDFPIAPGIYPNLKDAEKVVANAVFMENPSDEQFRKLKEDFRKIFL
                     TQ"
     gene            complement(1240..2295)
                     /locus_tag="VF089_13840"
     CDS             complement(1240..2295)
                     /locus_tag="VF089_13840"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6175094.1"
                     /translation="MSKDTPIRTSSPIGHSVTDATLRSCPDTVLWGYLAANLPPALSV
                     KSGAVVEVEALSHQGLTTQNDPEKFFASYGIPAAEVLSDAKAVFAGVNRPKGASVHIL
                     TGPIYIEDSEPGDTLEVRVLDIRFRVPYGVNNTGPGKGVLPKLLKEQSAKLIRLDLER
                     RVALFSSDIEIPLNPFMGIMAVSPPTSLGMVSSTPPGAWGGNMDLKFLGVGSSLFLPV
                     FNKGAQFFTGDGHAVQGDGEVDGGAIEISLAPKLQFLLHKRKTIKLPRVETATDYLLT
                     GLSVDLNEAARIALQETVDFLQAEKGLSAADAYALSSLAVDLGIGEAVDIVNLVYAKI
                     PKNIFRSNPPFWASTER"
BASE COUNT          596 a          665 c          645 g          543 t
ORIGIN      
        1 actcgcacaa aagattcgcg acagtttcat tgatcggcct gagcttgctc ttcttgctga
       61 gcaatcagct ttttgctcaa cccgtcgatc aggaggcggc gaaaaaggaa ggcaggattg
      121 ttgtctatgg cacgacgatc ccgaaggtca tggcgcctat tcatgccgga tttgaaaagc
      181 ggtacggagt taaagtcgat tattggcgcg catccgccac cgcggtcacc gaccgagcga
      241 tcactgagtg gcgcgccggc aaaccgggct tcgacgttgt atttgcaatc aacggaacga
      301 taactctgct taaacgcgaa aatgcgctgg ccaagttcac cccacccgcg gcggccaagt
      361 ttcccgccca tctgaaagac aaggatggcg tgctgaccgc gttccgccac actcctgtta
      421 gcattctcta caacacggaa ctggtgaagc ccgccgatct gccaaagggc ttcgatgatc
      481 tcttggatcc aaaatggcga ggcaaaatcg ccatgcccga tccttctaga cacaccagta
      541 cggcgcaatt tctctggaac atgcataaag ttaaaggtga gaagtggctc gattatgcca
      601 gagcactcgc caaacagaaa ccttttctgc tggaatcctt cgcaccggtg ccgaccgcct
      661 tggtaaaagg tgaagcgcaa attgggatta cttacgcgca atatattacc caggtgaagg
      721 gaccgctgag ccacgtcgtc ttcgataaag ttttgaccga ctcgaccgat ctcgctctca
      781 gcgcgaaagc tgcagctccc aatgcggcca agctttacat agattatctc tgcacggcgg
      841 atgtacaaaa gattatcgct gatagcggcg acttcccaat agctcccggg atctatccca
      901 atctcaagga cgccgagaaa gtagtcgcta atgcagtgtt catggagaat ccgtcagacg
      961 aacagtttag aaaactgaaa gaagacttcc gcaagatttt cctgacgcag taaagagaat
     1021 caagcccttg ttccctgtta gcacgcctgg tgttcccatg cggcgcaaca ttcgtctcct
     1081 tgcgcggtag cgaccagcct agacggtatt cgcgtgtatt caataatcgg cgatggtcgc
     1141 ggtcttagaa ggcctgaatc gatcgaggta tttcaaattg ctttcgatcc taaaagggtc
     1201 ggcgtgttcc ctggaagatt ggcggaccac tggtgaccgt catcgctctg ttgaagccca
     1261 aaaaggtgga ttgcttctga agatgttctt aggaattttg gcatagacga gattgacaat
     1321 gtccaccgct tcgccgattc cgaggtccac ggccaagctt gataacgcat acgcatcggc
     1381 ggcgctcaaa cctttctcgg cctgcagaaa gtccacggtt tcttgcagcg cgatgcgagc
     1441 cgcctcgttt agatcaacgc taagccccgt taataaataa tcggtggccg tttcgacccg
     1501 cggcagcttg atggtcttgc gtttgtgaag aaggaattgc agcttcggcg ccaatgagat
     1561 ttcgattgcg cccccatcga cctcaccgtc gccttgaacc gcgtggccgt cgccggtaaa
     1621 gaactgcgcg cccttgttga acaccggaag aaatagcgac gatcccacac cgaggaattt
     1681 caaatccata ttgccgcccc aagcgcccgg cggtgtcgaa gaaaccatac caagactggt
     1741 tggcggcgac acggccatga ttcccatgaa cggattgagt ggaatttcga tgtcgctaga
     1801 gaagagtgcg actcgccgct ccagatcgag ccggatcagt ttggcggact gttctttcag
     1861 cagcttgggc agcacacctt taccaggccc ggtattattc acgccatacg gcacgcggaa
     1921 tctaatatcg agcacgcgaa cctccaaagt atcgcccggc tccgaatcct cgatgtaaat
     1981 cggcccggtg agaatatgaa cgctagcccc tttcggacga ttcacaccgg caaaaaccgc
     2041 ctttgcgtca gagagaactt ccgcagcggg tataccgtag ctggcaaaga atttttcagg
     2101 atcattctgc gtcgtcagcc cctggtgcga gagcgcttca acctcaacga ctgcgccgga
     2161 tttgacgctt aacgccggag gaagattcgc tgcgagatag ccccacagaa cggtgtccgg
     2221 acaggaacgt agcgtcgcat cggtaaccga atgtccgata ggagaactcg tgcgtatcgg
     2281 tgtgtctttg ctcatattgc ccggcaccct agcggatatg agcgcaggct gtcaacgaag
     2341 atgggcgggt tgattaatca gaacgctatt ggttgctcta tgactaaagg gcaaccggtt
     2401 acaatgggcg ttaaagcagc ttacgcggat ttgtagaagc tctttacag
//
LOCUS       DASUTG010000601         4075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_389444, whole genome shotgun sequence.
ACCESSION   DASUTG010000601 DASUTG010000000
VERSION     DASUTG010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389444"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF089_13845"
     CDS             <1..365
                     /locus_tag="VF089_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175095.1"
                     /translation="DMAGVINSDGGAMQFCRMYTGDDGKSHFEELEQDAGSKLFSNSL
                     SVKTLVFKNDENREILGWHNAPRRQWCITLSGSVQIGIGDGTVKTFGPGDVFLAEDVT
                     GQGHTALPKDWVRAFVHL"
     gene            568..1593
                     /locus_tag="VF089_13850"
     CDS             568..1593
                     /locus_tag="VF089_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175096.1"
                     /translation="MHRMRFVGRLSAVFLLSGLLSPPNSAAQDKIRVGLSSVSATSGS
                     IWVAEEKGLFKKHGLDAEVIIIGGGASRVVSSLLAGEIQVSVGGGDAVIRAALRGADG
                     VLAASPMKTGLQRMMVRPEIKTPADLRGKTIAVTRFGSASHWVLQLFLRKWGMRTDDI
                     KMLQLGSSPAMLASLEKGAVDGAVFTIPTFFLAEDRGYRTLADPVEMEIYYLQNSVDT
                     TRGYIKKNRDQSLRLIKALCEGIAYFRKNKKASLEVLQKKLRIQSAQERDVKYLEQSY
                     ELLADRFYSRVPYANPKAIETTLEFIAAEEPKAKDVEASQFIDDSLVREVEASGFIKE
                     LYEKEYR"
     gene            1739..2209
                     /locus_tag="VF089_13855"
     CDS             1739..2209
                     /locus_tag="VF089_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175097.1"
                     /translation="MTLPTFFVAEDRGYRIVGDPVTMDIFYLQNTLESTRSFLRKNRD
                     AALRFMRGYIEGIAYFKKNKSESLDVMRKKLRIQSEQERDNRYLEQSYNLLATAYNDI
                     PYPALKSVQTIVDKIAEDEPKVKERDVRSFVDDSLVKELEDSGFAKAVYGRQSE"
     gene            complement(2271..3077)
                     /locus_tag="VF089_13860"
     CDS             complement(2271..3077)
                     /locus_tag="VF089_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175098.1"
                     /translation="MPKLKANDIEIYYEEHGKGRPMVFLSETACDGEVWKINQVEEFS
                     RDHRVIIHDYRGTGRSSKPSIDYTTTMFAEDVVALMDHLDAENAIVVGHSMGGRVAQL
                     LALDHPRKVHKLVLASTGAHYPLTKGLPLKICKEMIEWGYEKYERDHTILVGWTEEYV
                     KSHPEKIENYLKVRMHNLCPVEFYLRHLLARQSHDTSQRLRDIKQPTLILVGEGDRNM
                     TSEINHLMSSEVLAKGIPNSKLIVLPNERHSYFFSNPDEAHRLIREFIKD"
     gene            3235..>4075
                     /locus_tag="VF089_13865"
     CDS             3235..>4075
                     /locus_tag="VF089_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175099.1"
                     /translation="MKKIACSYSVFPIWLFFLMAAPGFVLAQQPTKLPFPYGPLGLNS
                     LPWIVAKEFSIFEKNGLDLDMVYVGASAVIVQSMLSGSSNIAGFGGPAVITNVLRGGD
                     IIQVAAMAPYFTQSLFVRPDIRDLRSLQGKKIGITRFGSVTDFALRTILERHNLKQVN
                     VLQMGGFPEAVAGLSRGAIDGAVFSPPHNFRMLKEGFRELVSRKDLRALGSGFLTQGI
                     VARRSFAATHREVIVRLIKSTVEGTKYAFTHENLTKRIIGKYLRITDEDLLRQSYLYV
                     LDTF"
BASE COUNT          953 a         1077 c         1065 g          980 t
ORIGIN      
        1 ctgatatggc cggagtcata aatagtgatg gaggcgccat gcaattttgc aggatgtata
       61 ccggcgatga cgggaagtca cacttcgaag agctcgaaca agatgcgggc tcgaagctct
      121 tctcgaactc attgtcggtg aaaacgcttg tttttaagaa cgacgaaaac cgcgagattt
      181 tgggatggca caatgccccg cgtcggcagt ggtgtatcac tttatcgggc tcggtgcaga
      241 tcggcatcgg tgatggcact gtgaaaacat tcggaccggg cgatgttttt ctcgccgaag
      301 acgtcaccgg ccaggggcat accgccttgc caaaagattg ggtgcgcgca ttcgttcatc
      361 tataagccta tcatttcagg cccgatgcag ttcgatccaa acgtgcttcg ataatgcctg
      421 ccatcgggtt caagccgctg cttcttccca taatgaacct aaaactcttg acgcttgcat
      481 aagctttcta ataacgttgc ggcagtttgc aggcataact tgcaaatgaa attcggattg
      541 catccgtaat ggtcgccgga ggttttgatg catcgaatgc ggtttgttgg caggctttcc
      601 gcggtatttc ttctttcggg cttgctgtcg cctcctaatt ctgccgcgca agacaagatt
      661 cgcgtcggcc ttagctccgt cagcgctaca tcgggatcga tctgggtcgc cgaagaaaag
      721 ggactgttta agaaacatgg gcttgatgcc gaagtcatca ttatcggcgg cggcgcctcg
      781 cgagtggtga gttccctgct cgcgggcgag attcaggtct cagtgggcgg tggcgacgcg
      841 gtgatccgtg ccgctctcag gggcgccgat ggcgtgctcg ccgcctcgcc gatgaagacc
      901 ggtttgcagc gaatgatggt acggcccgag atcaagacac cagccgatct cagaggtaag
      961 actatcgccg ttacccgctt tggctccgca tctcattggg tcctgcaact gtttctgcgt
     1021 aagtggggaa tgcgcaccga cgatatcaag atgcttcagc taggctcatc gccggccatg
     1081 ctcgcgagcc tggaaaaagg agccgttgat ggagctgtct tcaccatccc aacttttttc
     1141 ctagccgaag accgcggcta ccgaaccctg gccgacccgg tggagatgga gatttattac
     1201 ctgcagaact ccgtcgatac gacccgtgga tacatcaaga aaaaccgtga tcagtcgctg
     1261 cgcctgatca aagcgctgtg cgaaggtatt gcgtatttca ggaaaaacaa gaaagcaagt
     1321 ttggaagtcc tgcagaaaaa actgcgcatt caatcggccc aggagcgcga tgttaaatat
     1381 ctcgaacaat cgtacgagct tctcgctgat aggttctaca gccgggttcc ctacgccaat
     1441 ccaaaagcga tcgaaacgac gctcgagttc atcgccgccg aagagccgaa agcaaaagat
     1501 gtcgaagcca gccaattcat cgacgacagt ctcgtgcgcg aagtcgaggc tagcgggttt
     1561 atcaaagagc tgtatgagaa agaataccgg tgaaagaaag ctagagcgat gattattgga
     1621 gtcctggagt gttgaggttc attccactca gtactccatc actccactac tccaattccc
     1681 aaaggagccc gaatggccga ttataaggag attctataca ccaaacaacg cggccgtctt
     1741 gaccttgcct actttcttcg tcgctgagga tcgtggatac cgcatcgtcg gcgatccagt
     1801 cacaatggac atcttttatc tacaaaatac cctggaaagc acgcgaagtt ttttacgtaa
     1861 gaaccgcgat gccgctctca ggttcatgcg cggatatatc gaagggatcg catatttcaa
     1921 aaagaacaag agcgaaagtc tcgatgtcat gcggaaaaaa ctaaggattc agtccgaaca
     1981 ggaacgggac aatcggtatc ttgagcagtc gtacaatctg ctcgcgactg cttataacga
     2041 tattccgtat ccggccttga aaagcgtcca gacgatcgtt gacaaaatcg ccgaagatga
     2101 gcccaaagtg aaagagcgag atgttcgatc gtttgtagat gacagcctgg tcaaagagtt
     2161 agaggacagc ggttttgcca aagcggttta cggccggcaa tcagagtgat ctcgaatcgc
     2221 cgatgcctcc gcatgatgag cggattgtca ccgcagcgga atgctcaaaa ctagtccttg
     2281 ataaactcac gaattaacct atgagcttcg tcaggattgg agaagaaata gctatgacgc
     2341 tcattgggca gtacgatgag cttggaattg ggtattcctt tggccaagac ttccgaggac
     2401 attaggtgat tgatctcgct ggtcatgtta cgatcgccct cgccgaccag gatcagcgtc
     2461 ggctgcttta tgtcccttag tcgctggctg gtatcgtggc tttggcgcgc gagcaagtga
     2521 cgcaagtaga actctactgg gcagagattg tgcattctta cttttaaata gttttcgatc
     2581 ttttcgggat gagatttgac atactcctcg gtccaaccga ccaagatggt atgatcccgt
     2641 tcgtatttct cgtatcccca ttcgatcatt tccttacaga tctttaacgg caagcctttg
     2701 gtcaatgggt aatgcgcccc ggtcgaagct aacaccagtt tgtgaacctt gcgtggatga
     2761 tcgagggcga gcaattgcgc cactctaccg cccatcgaat ggcccacgac gatcgcattt
     2821 tcggcgtcga gatgatccat caacgccacg acgtcctcgg cgaacatcgt cgtggtgtaa
     2881 tcgatggacg gtttgctcga ccgcccggtg ccccgatagt cgtggatgat tacccgatgg
     2941 tcccgggaaa actcctcgac ttgattgatc ttccaaacct cgccatcgca ggcggtttcg
     3001 ctcagaaaca ccataggacg gcctttgcca tgctcttcgt agtaaatttc aatgtcattc
     3061 gctttgagtt ttggcatcgt tcctcctagt ggcccgctcg ctttccggct gagtcgatgc
     3121 atattccaag taaatctgct tgacaagatc cgccccgaaa tttcttgact aaagactcac
     3181 cgtagcgata ttccaaaacc tgccgacagt tctctcggcg aactcggagg cgcaatgaaa
     3241 aagatcgcgt gttcctactc agtttttccg atctggttgt ttttcttaat ggccgcacca
     3301 ggctttgttc tcgcgcaaca accaaccaaa ctcccattcc cctacggccc cctcgggctc
     3361 aacagcctgc cgtggatcgt ggcgaaagaa ttcagtatct tcgagaagaa tgggctcgac
     3421 ctggacatgg tttacgtcgg cgcgtcggcg gtgatcgtcc agtcgatgct ttccggctcg
     3481 tccaatatcg ccggtttcgg cggtccggcg gtcatcacca atgtcctgcg cggcggcgat
     3541 atcatccaag tcgccgcgat ggcgccttac tttacccaat ctttgttcgt tcgtcccgat
     3601 attcgcgatc tgcgcagcct ccaagggaaa aaaattggca tcacccgctt cggctcggtg
     3661 acagatttcg cgcttagaac catccttgag aggcacaacc tcaagcaagt gaacgttctt
     3721 cagatgggcg gcttcccgga agcggtggct ggcctctcgc gcggagccat cgacggcgcg
     3781 gttttttcac cgccgcacaa ttttcgcatg ctcaaagaag gctttcgcga attggtctcg
     3841 cggaaggatc tgcgcgcgct cggcagcgga tttctcaccc aagggatcgt cgctcggaga
     3901 tcctttgccg cgacccaccg cgaggttatc gtgcggctga ttaagtccac ggtcgagggg
     3961 acgaaatacg cattcactca cgaaaactta actaagagga tcattggcaa gtatctccgc
     4021 attacagacg aagatctctt acgccagagc tatctttacg tccttgatac tttcg
//
LOCUS       DASUTG010000602         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2020091, whole genome shotgun sequence.
ACCESSION   DASUTG010000602 DASUTG010000000
VERSION     DASUTG010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020091"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF089_13870"
     CDS             <1..291
                     /locus_tag="VF089_13870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175100.1"
                     /translation="EYETRGGRSFPNIKKKPSEYVKDCAVYVTCEPEETSLAYVTQVI
                     GDDRLMFPSDYPHERVYGEFLKDIPELLARPELSDATKQKILYGNAKEFYRI"
     gene            302..1306
                     /locus_tag="VF089_13875"
     CDS             302..1306
                     /locus_tag="VF089_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175101.1"
                     /translation="MSISTRMIALLVLWSIVPAAFAQDAKLIEAAKKEGRLVLYGTMQ
                     TEIFELLQKSFQKKTGITIDYWRTSATRVTERALSEARAGKAFFDLVMSTEDTTRIML
                     KEGILAKYDSPMSKDFAKDAIDPQLGPRARNHIIGILYNKAIIKPGDAPKTLEDLVKP
                     QYRGKLVMADPTQHTTTAQWLVNVHKLIGKDKSEKFVRDLAAMKPTLVESFNPAAERV
                     TSGEFPIAITYVYYVYLHGIKGAPIDYVRTGKFLGDASYLTLHNKAPHPNAAKAFIDF
                     FLDEESMSIMAKAGEFVNRKGIYPPVPDADKIQFVEMDDLGEKVYAEKRKEFHKLFLR
                     "
     gene            1388..1876
                     /locus_tag="VF089_13880"
     CDS             1388..1876
                     /locus_tag="VF089_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HEX6175102.1"
                     /translation="MTFDYSMDFSAIDFRKQPELYRIGKGEQGVLLVEPYKSEILPHW
                     KFRTPQMARNSANRIYKMFLDYQAKDDFVGMDMARKFLQMGYTRSRRYANHTSGRKYA
                     SSTEELLPREVDHEKAQSARIFHQKWQKARKDGKYLQLRSRHREIYERNAENKRRGSA
                     PG"
     gene            complement(1950..2114)
                     /locus_tag="VF089_13885"
     CDS             complement(1950..2114)
                     /locus_tag="VF089_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175103.1"
                     /translation="MVSLKKSMVPALVAVLLLGGLAACDREGPLERAGEKVDESVERA
                     GDKVERATDK"
     gene            complement(2779..3744)
                     /locus_tag="VF089_13890"
     CDS             complement(2779..3744)
                     /locus_tag="VF089_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175104.1"
                     /translation="MGESPVLLDDVIITSQLSLRPTRSPDYRAEIQNIKILVATMSEN
                     PQAFWRRLAEAALELCRAGTAGVSLLDIEDGAEVFRAEAVAGVLGDRLAGTIPRPSPF
                     DTAIDRDATQLMYLPGRLFPKLRFDPPILEMLIIPFRVATMPAGAVWLAAHDDTCRFD
                     REDERIGKTLASFAAAGWHLRRTRRNAEPAIEHHPSSKSHGSRAGAEGKIEPRHMREE
                     LQQLSQSLEARASEKTAELIAGEGRIAMPQEDESQQSWLAENATAQSEIGDFDRLLTI
                     IQGYTTIMKSDLDDPAKLKEDIEAISEAASLVQALINSQWAQRLG"
     gene            complement(4324..>5695)
                     /locus_tag="VF089_13895"
     CDS             complement(4324..>5695)
                     /locus_tag="VF089_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6175105.1"
                     /translation="IVDLVINHTSNRHPWFQSARKDPASAYRNWYLWSKTRPAEWNKG
                     MVFPGVQKATWTRDPVADEYYFHRFYEFQPDLNTLNPAVQREMMRIMGFWLQLGVSGF
                     RMDAVPFMIEQKGAGVTPRKDYEMLHLLRNFLQWRRRDAIFLAEANVPPNESMDYFGE
                     NGERIQMMLNFAVNQRLFYALATEDIKPLVRALEATYNRPPAAQWVNFLRSHDELDLG
                     RLTDTQRERVFQAFAPAKEMQLYNRGIRRRLAPMLDNDRRKLELAFSLLFTLPGTPML
                     QFGDEIGLGDDLSLPERECARTPMQWSSDPHGGFSTGKRPVLPVISDPIYGYQRVNVE
                     AQRRDPHSMLNWMERKIRMRRECPEISWGDWKILKTDQPGVLVMHYEWDDHTLVTLHN
                     FTGKPCAVVLDRGALGGDIGAGELVDLLATNDSRADENGRYVIELQPYDYRWLRAGGI
                     DRNVPR"
BASE COUNT         1344 a         1583 c         1516 g         1252 t
ORIGIN      
        1 gaatacgaaa cccgcggcgg ccgctccttc ccgaatatca aaaaaaaacc gagcgagtac
       61 gtgaaggatt gcgcggtcta tgtcacctgc gaaccggagg aaacatcgct ggcctatgtg
      121 actcaagtaa tcggcgacga ccggctgatg tttccgtcgg attatccgca cgagcgcgtc
      181 tacggggaat tcctcaaaga catacccgag ttgctcgccc ggccggagtt aagcgatgcg
      241 acaaaacaga aaattcttta tggcaatgcc aaagagttct accgcatcta acggagagtc
      301 gatgtcgata tcgacgcgaa tgatagcgtt gctcgttttg tggagcattg tccccgccgc
      361 tttcgcccag gacgctaagc tgatcgaagc ggccaagaag gaaggccggc tggtgcttta
      421 cggcaccatg caaaccgaaa tttttgaatt gctgcagaaa agttttcaga agaaaacggg
      481 gatcacgatc gactactggc gcacttccgc caccagggta acggagcggg cgcttagcga
      541 agcgcgcgcc ggaaaagcct tttttgacct ggtcatgtcc acggaagata cgacacgaat
      601 catgctcaag gaaggtattt tggcgaaata cgactcgccg atgagcaagg atttcgccaa
      661 agacgcaatc gatccccagt tgggcccgcg agcgcgtaat cacattatcg ggattctata
      721 caacaaagcc atcatcaaac cgggtgatgc tccgaagaca ctggaagatc ttgtcaaacc
      781 tcaataccgc ggcaaattgg tcatggccga ccccacgcag cacacgacga ctgcccaatg
      841 gctcgtcaac gtgcacaaac tcatcggtaa agacaagtcg gaaaagttcg ttcgcgactt
      901 ggccgcaatg aaaccgacct tggttgagtc cttcaacccc gccgcagaac gggtcacctc
      961 gggagagttt ccgatcgcta tcacttacgt ctactacgta tatctacacg gcatcaaagg
     1021 agcgcccatc gattacgtgc gaaccggtaa gtttctcggc gatgcgagtt atctgacatt
     1081 gcataacaag gcgccacatc ccaacgccgc taaagctttc atcgatttct ttctcgatga
     1141 agagagcatg agcatcatgg ccaaggccgg cgagttcgta aatcgcaagg gtatttaccc
     1201 gccggtaccc gatgcagaca agattcagtt cgtcgaaatg gacgatttgg gcgagaaggt
     1261 gtacgctgag aagcgtaaag aatttcacaa actatttctt cgataagagt tgagtgtcat
     1321 ttgacacgcg ctgctcgaac gcgtttcgcg gtgatagcgc atggaccggt tattcagccc
     1381 aagctctatg acattcgatt attcaatgga tttctcggca atcgatttca gaaagcaacc
     1441 tgaactctac cgcatcggta agggcgaaca gggagtactc ctggtcgaac cctacaagag
     1501 tgaaatactg cctcactgga aatttagaac gccgcaaatg gctcgcaact cggccaaccg
     1561 aatatataaa atgtttctcg actatcaggc aaaagacgat ttcgtgggga tggacatggc
     1621 tcgcaaattt ctgcagatgg gttacaccag gtcacgaagg tacgccaatc acacatcagg
     1681 ccgtaagtac gcctcgagca ctgaagaact actgccccga gaagtagacc acgagaaagc
     1741 acaatcggcc agaatttttc atcagaaatg gcagaaggcc agaaaggatg gaaaatactt
     1801 acagctgagg agtcgacacc gagagattta tgaacgcaac gcagagaaca agcgaagagg
     1861 atcagcacct ggatgagaag cgggagtcgt cacggccact ttcggtgcgg tgcgaacttc
     1921 gcaccgcacc ggtgaactca tcgtacggct tacttgtccg tcgctctttc caccttgtcc
     1981 cccgctcttt caacggactc atcgaccttt tcgccagccc gctccagcgg cccctctctg
     2041 tcgcaagcgg ccagtccgcc gagcaacaga accgcgacca gtgccggtac catcgatttt
     2101 tttaaactta ccatgagatg tcctcctctt gtgatgattc gtggcagcca gccgctcaac
     2161 tgcggcagcc ggcgatccac accgcgttcc acccgcagct tccatgccac agaggaggca
     2221 caacgagcga taggtctgta gatcagtaat cgagggcttc gttccgatcg ggaagtaaaa
     2281 tctccaaaat gacacgctgc tgcaggcgtg tgatgcaaga gcttcttttg tcccgtaact
     2341 cagacacaga ggctcagaat tgagacagct gcatatatcc aacgtccagc ttgcatctag
     2401 ggatcatgac cgccgtatta gcactctcac acctctaagc cattacgcct gaatgtttga
     2461 gggtaccgct attggaaggg aagcttcaga gacgtggatc accatgggag aaaatctttc
     2521 aattctatcg ccggagcggc tattcggaaa agatcctgtg agccgatgtt gtagcgcctg
     2581 gcaatgcctg ccgcgcacac tcgccagggt ggcttgcgca gagtagcgct cattcgcttc
     2641 agccggcatt cttaatcgtt gctatgctgg aactgctctc aattatcttt tatgtggccc
     2701 ccgggcggta atccgatgcg gactcgtgtc aggcaatctg caatcactga gtgcgactgc
     2761 tctgggcaga tggaagtgct agcccagacg ctgtgcccac tgagaattga tcaaagcttg
     2821 aaccaacgat gccgcttcac tgattgcttc tatatcttct ttgagttttg ccggatcgtc
     2881 taagtcggat ttcataatcg tggtgtatcc ctggatgatt gttagcaggc ggtcaaaatc
     2941 acctatctcg ctttgcgccg tcgcattttc agcgagccac gactgctgcg actcgtcctc
     3001 ctgaggcatc gcaatccggc cctccccagc gatcaactcc gctgtttttt ccgaggctcg
     3061 agcttcgagg ctttggctca gttgctgcag ctcttctcgc atatgccttg gttcgatctt
     3121 cccttctgct ccagcccggc tcccatgact cttgctactt ggatgatgct cgatggcggg
     3181 ttcggcgttg cgtcgcgttc ttcgtaagtg ccaacccgcc gcagcaaaag acgcgagggt
     3241 ctttccaatg cgctcgtcct cgcggtcaaa cctgcaagta tcatcgtgag cggccagcca
     3301 aacagcgccg gctggcatag ttgcaacacg aaaaggaatg atcagcattt caaggatcgg
     3361 cggatcaaat ctcagtttcg gaaataatcg ccccggtaaa tacatcagct gcgtcgcgtc
     3421 acgatctatg gctgtgtcaa acgggctggg acgcggaatt gtgccggcaa gccgatcacc
     3481 gagaacaccc gcgactgctt ccgctctgaa cacctctgct ccatcttcga tgtctaatag
     3541 gcttacccca gcagtaccgg cgcggcatag ctctaaggca gcttccgcaa gcctacgcca
     3601 gaaggcttgc ggattctcgg acatcgtggc aacgagaatc tttatatttt gtatttcagc
     3661 tctgtaatca ggtgatcgcg tgggtcggag agaaagctgc gacgttatga tgacatcgtc
     3721 caacaacact ggtgactcgc ccattgtgtt cctcacggcc ggtcgtacgg cgttgggctt
     3781 taacgcggcg caagaagtcg ctcatggacc ggaaatgcct gcggtagtcg agagaatcaa
     3841 ctgaaactaa aaggctcttt gaatacatcg cacggaggtt tgtatatcgt gcgcagctat
     3901 ttcgagtcaa caataattag caatacgcct ctgtggaccg gcattcgttc agaaactcgt
     3961 catgattggc gctgctttac cgcggcttcg atgatctcga ccgcgcccgg gcagctcttc
     4021 ttagcgctct cgagcaaaat gcgagcatct cgtttatcgc aggccagttc ataagtctca
     4081 tcaaatagag ggccagacga gcgggtatga gcggcgcgat ccaggagcgt atgaaccctt
     4141 gactggactg gcaaagagga cagcagggac tctctatgtt ccggaatcaa ggtgatgcgt
     4201 actttcatca tgacaggaac aagttaagtt gcgccgatcc acttggcaag ggaggggaaa
     4261 cgagggtggg cctatcgttt caaccgtgtg cggagcggct caaaagccta aagcaccgca
     4321 gtttcatctc ggcacgttgc gatcgatgcc tccggcgcga agccagcgat aatcgtaggg
     4381 ctgtagctcg atgacataac ggccgttctc gtcggcgcgg ctgtcgttgg tcgcaagcag
     4441 atcgacgagt tcaccggcgc cgatatcacc gccaagggct ccgcgatcga gcacgacggc
     4501 gcacggcttg ccggtgaagt tgtgcaatgt gaccagcgtg tggtcgtccc actcgtagtg
     4561 catgacgagc acaccgggct gatcggtctt aagaattttc caatcacccc aactgatctc
     4621 cggacactcc cgccgcatgc gaatcttgcg ttccatccag ttgagcattg aatgcggatc
     4681 gcgacgctgc gcttcgacgt tgacccgttg gtatccatag attggatccg agattaccgg
     4741 cagaacgggg cgcttcccgg tcgaaaatcc accgtgcggg tcgctggacc actgcatcgg
     4801 cgtccgcgcg cactcacgtt ccgggagtga cagatcatcg ccaagcccaa tctcgtcacc
     4861 gaactgcagc atcggcgtgc ccggcagggt aaacagcaga ctaaatgcca gctcgagctt
     4921 gcggcgatcg ttgtcgagca tcggtgcaag acggcgcctg atgccgcgat tataaagctg
     4981 catctccttc gccggcgcga acgcctggaa cactcgctcg cgttgcgtgt cggtcagcct
     5041 gccgagatcg agctcatcgt ggctgcgaag gaagttcacc cattgcgccg ccgggggccg
     5101 attgtaggta gcttccagcg ctcgcacgag cggcttgatg tcctcagtcg ccagcgcgta
     5161 gaacagccgc tgattgacgg cgaagttcag catcatttgg atccgctccc cgttctcgcc
     5221 gaagtagtcc atgctctcat tgggcggaac gttcgcttcc gcaagaaaaa tcgcgtcgcg
     5281 ccggcgccac tgcaaaaaat ttcgcagtag atggagcatc tcgtaatctt tccgcggcgt
     5341 gacacctgcg cctttttgct cgatcatgaa tggaacggcg tccatgcgga agccggaaac
     5401 gccaagctgc agccagaatc ccatgatccg catcatttcg cgttgtacgg cgggatttaa
     5461 cgtgttcaga tctggttgaa attcgtagaa tcggtgaaag taatactcat ctgcgaccgg
     5521 atcacgggtc cacgtcgctt tctgcacgcc cggaaatacc attcccttat tccactcggc
     5581 tgggcgcgtc ttcgaccaga gataccaatt ccggtaagcc gatgctggat ccttgcgcgc
     5641 agactggaac cagggatgac ggttcgacgt gtgattgatc accagatcga cgatc
//
LOCUS       DASUTG010000603         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_834240, whole genome shotgun sequence.
ACCESSION   DASUTG010000603 DASUTG010000000
VERSION     DASUTG010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834240"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1206)
                     /locus_tag="VF089_13900"
     CDS             complement(187..1206)
                     /locus_tag="VF089_13900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6175106.1"
                     /translation="MKLLKPFVAIILGLVVVPFADAQEPFYTGKTVRLVVGFSAGGGF
                     DVYSRTISRHIGKHLPGNPTVIVENMTGAGSLVAANHVFKVAKPDGLTIGNINGGIFM
                     QQLLGWPGIEFDALRYEYIGVPVKDKSVCVMTKASGFTSFEKWIASKSPLKLGATGPG
                     SATHNVPLILKEALKLPIQLVSGYKGIADVRLAAESGELDGVCGWTWDSLKATWTRAL
                     DSGDAIVVLQTVSRPIAELPKVPLAISAAKTEESRQLIQSGIHDVSDLTYSYVLPPGT
                     PKDRVEIVRKAFAATMKDAEFIADAKKSKLGVDPMTGDELEKTVSGLFKLAPGVVSKL
                     KEVLK"
     gene            complement(1337..2362)
                     /locus_tag="VF089_13905"
     CDS             complement(1337..2362)
                     /locus_tag="VF089_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6175107.1"
                     /translation="MKFMKLLKALLIFCFLFGALTASSAQEPFYKGQTIRFIVGLSAG
                     GGYDLYSRTIARYLGKHIPGNPTVVVENMVGAGSVLSANYLYRAAKSDGLTILNFLGG
                     LFLQQLMGKPGIEFDSRKFEYLGVPGQDHFVITVAKSTGIQDVKEWIASKKEITMGGV
                     TPGGGTDDLPKVLKTTIGLPVRVVSGYKGTAEIKLAFNSGEVAGVSSAWESLKVTWSR
                     EYLSGDAVIVLQVSLKSHPELARIPLAFDFIKNDADKRLLEAVIRVHGPSVRPYVTAP
                     GTPKARVDLLRKAFANTMKDPEFLADAKKSKLDVNPLTGEELQENVREIFNLEPALVE
                     KLKQILK"
     gene            2522..>3291
                     /locus_tag="VF089_13910"
     CDS             2522..>3291
                     /locus_tag="VF089_13910"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6175108.1"
                     /translation="MNATFRIVLIVLAFLILISAWGFYISIRPPRIISSITPSAYNMQ
                     YENVSFETADGITLRGWHVPSQKKTDKTLILLHGYPADKGDILPALAFLQKDFNLLLF
                     DFRYLGQSDGSYSTAGAKEVEDLLAAIRFLKSRGITEVGIWGFSMGGAVALMAIEKAP
                     EIKAVVAESSYASLSDMALQFFRVRLINYPIAYLIGLWAKLFIGIDLKDASPAERIRS
                     STIPILITHSSTDAVIPFSQAKLLQQALVNNSRAEFWF"
BASE COUNT          773 a          903 c          816 g          799 t
ORIGIN      
        1 aaatgccaat accctcttct tctcgcgcca acgtcacgtg agtcagttcc gggtcggcgt
       61 ccacaagcgc caggagtttc tgcagattgg catccggcag ggatgtgacg aagctgacac
      121 cggcttcttt catgccttca acgattaatt gaggtccgtt gtcttccgcc atatcgattc
      181 cgcgctctat ttaagaactt ccttcaactt gctcacgacg ccgggagcga gtttgaaaag
      241 cccactgacc gttttctcca attcatcgcc ggtcatagga tcaacaccca atttggactt
      301 cttggcgtcg gcgataaatt ccgcatcctt catcgtggcg gcaaaagctt tgcgcacgat
      361 ctcgactcga tctttcggcg tgcccggcgg caggacatag gaataggtca aatcggaaac
      421 atcgtgaatg cccgactgga tgagctggcg cgactcctcg gtttttgcgg cgctgatcgc
      481 caatggaacc ttcggcagct ccgcaatcgg ccgtgatacc gtttggagga cgacaatcgc
      541 gtcgcccgag tctaaagccc tcgtccatgt cgcctttagt gaatcccagg tccaaccgca
      601 aaccccatcc aactccccgc tttcggcggc tagacgaaca tcggcaatgc ctttatagcc
      661 ggagaccagt tgaatgggca gtttcaaagc ttctttgaga atcagcggca cgttgtgtgt
      721 cgccgaaccc ggtccggtgg cgccgagttt tagcggtgac ttggatgcta tccatttttc
      781 aaagctggta aagccactgg ccttggtcat gacgcacacg gacttatctt tcaccggaac
      841 gccgatgtac tcgtacctta gcgcgtcaaa ctcaatcccg ggccaaccca ggagctgctg
      901 catgaagata ccgccattga tattgccgat ggtgagaccg tcaggcttgg cgactttaaa
      961 aacatgatta gcggcaacca gactacccgc gccggtcatg ttttccacaa tgacggttgg
     1021 attaccgggg agatgtttac ctatatgcct ggaaatcgtt ctgctataaa catcaaaacc
     1081 accgccggcg ctgaatccca cgacgagccg cacggttttt cccgtataga aaggctcttg
     1141 cgcgtccgcg aatggcacga cgaccaatcc caagataatt gccacaaaag gcttcagcag
     1201 cttcatagtc cccttcacag ttttgtgttg agaagttaat gccgatctgc gaagtctggt
     1261 gaatcggcaa aacagactca taaatagaca ggtcaaagtt cgattcgcga aagttcgcct
     1321 gcttactggt tcgcagttac ttcaaaatct gtttgagctt ttccaccaac gcaggctcga
     1381 gattgaaaat ctccctgacg ttttcttgca actcttcacc ggttaatgga ttgacgtcga
     1441 gcttggattt ctttgcatcg gcgagaaatt ccgggtcctt catagtattg gcaaaagctt
     1501 tgcgtaggag atcgacacga gccttcggtg ttccgggagc ggtgacatat ggacgcacgc
     1561 tcggcccatg cacgcggata accgcctcga gcaatctttt gtccgcatca tttttgataa
     1621 aatcgaacgc caaagggatc ctggcaagct ccggatgaga ttttaaagat acctgcaaaa
     1681 caataaccgc gtcgcccgac aggtattcgc ggctccacgt gacctttaag gattcccacg
     1741 cgctgctgac gcccgctact tcgccgctgt tgaaggcaag ctttatttcc gctgttccct
     1801 tgtaaccgga gacgacgcga acgggcaaac cgatggtcgt tttcagaacc ttcggaagat
     1861 cgtcggtgcc gccgcccgga gtaactccgc ccatggtgat ttcttttttt gacgcgatcc
     1921 actctttgac atcctgaatt ccggtggatt tcgccactgt gatgacaaag tggtcttgtc
     1981 caggaacacc cagatattcg aatttacgcg aatcaaattc gatacccggt tttcccatga
     2041 gctgctgcaa gaacaaaccg ccgagaaaat taaggatggt gaggccgtcc gactttgccg
     2101 cccgatagag atagtttgcc gatagcacgc tgccggcacc gaccatattt tcgacgacga
     2161 ccgtcggatt gccgggaata tgcttgccca gataacgggc gatggtccgc gaataaaggt
     2221 cgtatccgcc gccggcggaa agccccacga tgaaacgaat ggtttggcct ttatagaaag
     2281 gctcctgtgc actgctcgcc gttagcgcgc cgaagaggaa gcagaaaata agcaaagctt
     2341 tcaggagctt cataaacttc ataaacttcc tttcctcgcg atgatgctcg gagaccactc
     2401 gaacctattc gaaacgagcg tttcatgtca atgcaggcgt gcagcaaact tcccgagcag
     2461 cacgcgctca tcttgcaaaa tcagttgaca ctttcccgca gctcagaaac aatcactgag
     2521 ggtgaacgcg actttccgca ttgttctcat cgtcctggcg tttctgattc ttatcagcgc
     2581 gtgggggttt tatatctcca tccgtccgcc caggatcatt tcgtcaatca ctccaagcgc
     2641 ctataacatg cagtacgaaa acgtctcgtt cgaaacggcg gacggaatca cgttgcgcgg
     2701 ctggcatgtg ccatcgcaaa agaagaccga caagacgttg atcctgcttc acggctatcc
     2761 ggcggataag ggagacatac tgccggcgct tgccttcctg caaaaggact tcaaccttct
     2821 cctgttcgat tttcgctatc tcggccaaag cgacggtagt tattccacgg ccggagccaa
     2881 agaggtcgag gatctgctgg ccgcgattcg atttctcaaa agccgaggca tcacagaagt
     2941 ggggatatgg ggcttttcga tgggtggcgc ggtggctttg atggcgattg aaaaagctcc
     3001 cgagatcaaa gcggtcgttg cggaatcgag ctatgcaagc ctgtccgata tggctttgca
     3061 gttttttaga gtacgcttga tcaactatcc tatcgcttat ctaatcggtt tatgggctaa
     3121 gctctttatc ggcattgatc tgaaagatgc ctcgccggca gaaagaatcc gcagcagcac
     3181 gattccaatc ctaatcaccc attcgtctac cgatgcggtc attccatttt cccaggccaa
     3241 attgttacag caagccttgg tcaacaattc tcgcgcagaa ttctggttca a
//
LOCUS       DASUTG010000604         9271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1874978, whole genome shotgun sequence.
ACCESSION   DASUTG010000604 DASUTG010000000
VERSION     DASUTG010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874978"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VF089_13915"
     CDS             <1..1006
                     /locus_tag="VF089_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6175109.1"
                     /translation="ALILVGTSTSYAQTPYYQGKQIRLIIGSSPGGGYDLWPRMMLRY
                     LTKHIPGNPEIVPQNMPGAGGVVSANYVYGIAKPDGLTIGAFNPALYFDQLIKREEVK
                     FDWSKFAWIGSPEQNDILHFIRTDAPYKTVDDLRNAKEPPKCGSSGTGTTGHYIPRLL
                     EEVLGIKTHIVSGYQGGAEIDLAVERNEVVCWSPLVATYFGRDPYKRWHQSGFTRVVL
                     QTGQKRDPRLKDVPTIWELMAEYKTPDSGKRLAKVILTAATLGRPVTAPPGTPADRVK
                     FLREAYAKALNDPDLLAEASRRNWDVEPVSGEDLESRSKQVIAQPAEVIERMKWVLGK
                     "
     gene            complement(2290..2739)
                     /locus_tag="VF089_13920"
     CDS             complement(2290..2739)
                     /locus_tag="VF089_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175110.1"
                     /translation="MENADRVRANTAEEINRQIDRDIEIRVREYSQRTESEITRRINE
                     LEREWDIERLLETNASSLAFTGLVLGITRNRNWLIVPGIVLPFLFQHAIQGWCPPVPI
                     FRRFGVRTRDEINREKYALKALRGDFDEINSMRHPEEAEDALEAVKA"
     gene            3737..5059
                     /locus_tag="VF089_13925"
     CDS             3737..5059
                     /locus_tag="VF089_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6175111.1"
                     /translation="MTETELPRDQLLALASHLKSRRESILEKWRAAVKQDVALTNATT
                     LSSAQFYDHIPSVLDSFETNLCSRSQTAATDAAEVQKELAADHGLHRWHHGYNQQEVM
                     REWGHLHLCLVAELEEYDAAHPEIAHEVMAVARLTLAQLCSDGVNESASRYARLQQVE
                     AAGRLRDLEQALAQLRNLERQRADTWREAAHDLRGNLAVVKNVTEILTDERLPGSDLS
                     EIRTMLQQGVESLHALLEDLIVLSRLDAGHDRRNITRFDAGAMLRDLCAAMKPAAKER
                     NLSLESLGEETFMVEGDSVKVQRIAQNLLLNAIKYTEKGGVKVGWSEVAPQTYERWML
                     YVQDTGPGFEDGSVTPLAQAIKKSTEEVQVVDQEAEKAGQDSTFIAPAPVLPSESRHR
                     PDYQPPGEGIGLSIVKKLCELLDASLEFETKRGKGSTFRVIFPRRYEA"
     gene            5317..6378
                     /locus_tag="VF089_13930"
     CDS             5317..6378
                     /locus_tag="VF089_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175112.1"
                     /translation="MRTRIEGALPIAAVAVFLSIGSPAGSETKKPTTLAELAAYTGAD
                     REQLLLAGARVEGKVVWYTSLAGASFKEPMKAFEAKYPGVKVEVYRSQSSDLGKRITT
                     ENQAKRFYMDALETTLPLQKLLLEGKMMTPFTSPHLSKYPDIAKRKANDGLYFWAVDR
                     ESYSGLAINKTSISAAIAKNYEDLLRPEFKGKIGFAISETGPRAIGAMVKAKGEEFVR
                     KLRSQEVSLHAISGRAVADLVASGELGASPTVFRSHASEIAANGAPVTWIPMDLVPTN
                     AGAVSLPISAPHPHAAVLLIDFMLSPEVQTIFEKGQQGSPAKEYGFKRWYPEEGMNSE
                     QYDKASTQWEKLLREIGRN"
     gene            6414..7451
                     /locus_tag="VF089_13935"
     CDS             6414..7451
                     /locus_tag="VF089_13935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175113.1"
                     /translation="MLTIDADAHVVESERTWDYLEGNDRRYRPVPITVDMPLEKRKNF
                     WIIGGRLIGGRDNIGKDTSQESREMADIEARLRHMDEIGTGIQVLYPTLFLRPWTDQP
                     EIELALSRSYNRWLADIWHKAKDRLRWAVHLPLMTMDKALDELRWASGNGACAVFMRG
                     LETTHRLHDPYFFPLYEEASRLNMPIGIHSGVGNFAVSEAFGVDPFREAKLGVIGAFH
                     AMIMRGIPERFPGLRIGAIEVSAQWIPYVVHDINLRLPKFLGKEANPDVLRNSSQLFV
                     ACQTDDDLPYVLKYSGENSLMIGSDYGHADNASELLALNRLKAKGKIDPHVIDKILYD
                     NPARFYGLSIN"
     gene            complement(8181..8873)
                     /locus_tag="VF089_13940"
     CDS             complement(8181..8873)
                     /locus_tag="VF089_13940"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX6175114.1"
                     /translation="MCGRFTASFEFREIKVRYNLQQDLPFFGRRFNIAPSQEVPVIIQ
                     NGGKNELKIMRWGLVPSWAPDRSIANRMINARAETVTEKPSFRRLIESRRCLIPADGF
                     YEWRREGSGKVPVWFYIKKKEPFAFAGLWDVWRDVEGEILHTFTIITTSPNALLRRIH
                     NRMPVIFDALQARQWLDPRLSTRPADIAAVLAPFPSELMESHDVSPLVNKPEFDSSDC
                     INPISDEQLRLV"
     gene            8978..9139
                     /locus_tag="VF089_13945"
     CDS             8978..9139
                     /locus_tag="VF089_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175115.1"
                     /translation="MVTADSKCPQCGNGIMKLFPETKPLIPEPEQSELRARCTACNYE
                     DTVTIRSDD"
BASE COUNT         2265 a         2480 c         2387 g         2139 t
ORIGIN      
        1 ggctctgata cttgtcggga catctacctc ttacgcccaa actccctatt atcaaggcaa
       61 gcaaatcaga cttattatcg gctcgagtcc gggcggcggc tacgatctat ggccgcgcat
      121 gatgctgcgt tatctgacta agcatatccc cgggaatccg gagatcgtgc cgcaaaacat
      181 gcccggcgcc gggggcgtgg tgagcgccaa ctatgtctat ggaattgcta aaccggacgg
      241 tctgactatc ggcgctttca atccagcgct ttatttcgac caactgatca agcgcgagga
      301 agttaaattc gattggtcaa aatttgcctg gatcggatcg ccggaacaga atgacattct
      361 ccacttcatc cgcaccgacg ctccgtacaa aaccgtcgat gacctgcgta acgctaaaga
      421 gccgccgaaa tgcggcagct ccggtaccgg cactaccggc cattatattc cgcgtctgct
      481 cgaagaggtc ctcgggatca aaacgcacat cgtcagcggc tatcaaggcg gcgccgagat
      541 agatctcgcg gttgaaagaa atgaagtcgt ttgttggtca ccgctagtgg ccacatactt
      601 tggcagagat ccctataagc gctggcacca aagcggattt acgcgggtcg tccttcagac
      661 cggtcaaaag cgtgacccgc gattgaagga cgtgccgacc atttgggagc tcatggccga
      721 gtataagacg ccggattccg gcaaacggct ggccaaggtg atcttgacag ccgccaccct
      781 cggacggccc gtcactgctc cgccgggaac tcccgccgac agggtaaaat ttcttcgtga
      841 agcctatgcg aaggccctca atgatcctga tctgctagct gaagctagca gaagaaattg
      901 ggatgtggag ccggttagcg gcgaagattt ggagtcacga tcgaaacaag tcatcgcgca
      961 gcccgccgag gtcatcgaac gaatgaaatg ggttctcgga aaatgatccg ggccggttct
     1021 cagcgtcgag acgttggtta actttgcagg tcgtctagcc acgaattacg gctcgcgcct
     1081 cctcgagtac actgaaaagc cggcagcacc cgctcacgta gttgaaacct gtcacgaaac
     1141 attgcgtcgc ccgggttgac agggttcaca aagctttcat cccatcctca ctcttcattc
     1201 cggtatagct caatttgatt gccgcctgcg cctcccacgc aggtactatt cgaagcactc
     1261 gccggctcgt ctcaagcgca acgtgcggct actgatccta aatgtattag cgctcatggt
     1321 gataaccggt gactcctgaa cgcgacgaat ccttaatcac cgccgagctg attcggtacc
     1381 gttgccttcc atgagcgatc acactctata agcaattctt ggtactgcaa gcgcttagat
     1441 cccttatctc agcaatcagt tgtaagaccg ggaaaggaag gtacaaaatt gtagatcttc
     1501 gtcgattgat gtctacgggt gaaacctgcc tcaatgcgag tttcgtgacc tacgcggatc
     1561 aactccccta aaatattaga tttctttatt cgatatctcc gctattgatc tacatcatac
     1621 ccggcacaac ggttgctatt tagtcattca tcaagttcac cggaaactga tcataaggag
     1681 tttagcccgc agcgatgcta acctatcttt cgaaacgtaa gtgcccacag tgcgctttcg
     1741 actttgtcgt cgttctgctg gattgccact cccgaatgca gtggattaac ggtttttgcg
     1801 gacgatgcga tcacaatatc tcatggaagc tgtttcggag aagagattat gaacccagaa
     1861 atcattctgt ggactcgagt actgttacaa gccatatggg atctggccgg cataagggtc
     1921 aacgcaccac cgagagacgt gccgaggctc cggtcatcag cccgagcttg gatctgctcc
     1981 gctgatcact cgcaaggaag ctttatctgg acctgtaatt ctctttcact cgatcccgac
     2041 accgtccgaa agcgcgtgtt gaacaagagt aaagcagatc tgaaagcgat cacagagaaa
     2101 gaatttctcg aggggccact gcgattgatc aattcgatgc agtgacagaa tcagccgtga
     2161 gtaaagcgac cgctgcccgc gcaatccggt gcgggttggg tcgcccctcg gacgccggaa
     2221 acgcatgcaa ctcccatcca gcaatgactt accccatgca tttccagcga tgctggaagt
     2281 agttcgagac taagccttaa ctgcttcgag agcgtcttca gcctcttcag ggtgtcgcat
     2341 agaattgatt tcgtcgaaat ccccacgcaa agctttcaaa gcatatttct cccggttgat
     2401 ctcatcccga gtgcgaacac caaatcggcg gaatattggc acaggcggac accaaccctg
     2461 aatagcatgc tgaaacagga acggtaagac aatgccgggg acaattaacc agttcctgtt
     2521 gcgggttata ccgagcacaa gtccggtgaa cgcaagtgag gaagcgttag tctccagcag
     2581 ccgctctatg tcccactcac gctcaagctc gtttatacgc cgggtaatct cactctcggt
     2641 tcgttgggaa tactccctta cgcgaatctc aatatcccga tcgatttggc ggttgatctc
     2701 ttcagcagta ttggcgcgta ctctatctgc attttccatg tttattggcc tccggtaaat
     2761 cgaggatcca tctttctcct ctgcgtcagc tcacatcgat caaaaagcct gtagatcgtg
     2821 gctatctcga tgccgaacct cttatcacgg ctgatctgta ctttaacatc aagcggcggc
     2881 gaaaaaaatc gtctggcagg aaaaggcagg ctaaagatct ctatcggacg ttgctacctc
     2941 cttaaccaga aacatgcatt gctggcacct tacctcggaa acggtaccgt tattgtattc
     3001 ggtttgttta agatcttttg agccgcattc ggggcacttt gtcccttgat cagtagctac
     3061 atcatatcca agtttttcta tttctacatg ccctttccgt accggaagca tctcgatttc
     3121 ataatagagc tggcactgcc gacaaataag gcggcactcg cgaaataatc tcggccggta
     3181 ctcccttggt ttccactcgg tttcaacgac aaagtcctgc gactgacagt tggggcaggt
     3241 catcttaatt tccataaacc ctccttcgca ccggaaattg cagcgggcag ttcgacattg
     3301 tggatcccca taagcccagc tgcttcgtag aatcaactac ttatgatcgc ctgtcaataa
     3361 taaagctcat cccgaaggga tttgttcggc ttaatgacaa cgcgaaatga gaattgatcc
     3421 gcacgtgctg cgaaatcgcg cgatagtgtt attgcctggg gaaagagttc gatcgcgcga
     3481 ctccaaacac ccatgcattg catagatcaa ccaccgcaaa catgagagct tccgctattt
     3541 accattgacg aaatactgca tgactattct aatattctca cgccttactt actgacagtt
     3601 gacgttatca cccaaaaaat ttccaaagca tgcttgcttc agtgcacgcc tagaatcttc
     3661 aagtgcggcg gtttcccgac ttgaactaac tgagccgttc tgtcagtctt ttgtgaattc
     3721 tgatttgtga agatcaatga ctgaaacgga gctcccgaga gatcagcttc tggctctcgc
     3781 gtctcaccta aaaagtcgac gcgagagtat tctggaaaag tggcgcgcgg cggtgaagca
     3841 ggatgtagcg cttacaaacg ctaccacgct ctcgagcgcg caattctacg atcatatccc
     3901 cagcgtactt gattcgtttg aaacgaatct ttgctcccgc agtcaaacgg ctgctaccga
     3961 cgcggcagaa gtccagaagg aactggccgc tgaccatgga ctccaccgct ggcaccacgg
     4021 ctacaatcaa caggaagtga tgcgcgagtg gggtcatctg catctctgtc tcgtcgccga
     4081 gctcgaagaa tacgatgctg cgcatcccga gattgcccat gaggtcatgg cggttgcgag
     4141 gctgacactg gcgcagttgt gcagcgacgg tgtcaacgag agcgcctcgc gttatgctcg
     4201 tttgcaacag gtggaagcgg ctggacgtct tcgtgacttg gaacaggcgc tggcgcagct
     4261 tagaaatttg gagcgccaaa gagccgatac ctggcgcgaa gcagcccacg acctgcgtgg
     4321 caatttggca gttgtcaaaa atgtcaccga aatactgacc gacgagcgtt tgcccgggtc
     4381 ggacctaagc gaaatccgca cgatgctaca gcagggtgtt gagtcgttac acgcccttct
     4441 cgaagatctg atcgtattgt cgcgcttgga tgccggccac gatcgccgca acatcacacg
     4501 tttcgacgcc ggcgctatgc tgcgggattt gtgcgctgcc atgaaacccg ccgcgaagga
     4561 gcgaaatctc tctcttgaat cgttgggtga ggaaacgttt atggtcgaag gcgattccgt
     4621 caaagttcag cgtatcgcgc agaatttgct gctaaacgcc atcaagtata cggaaaaagg
     4681 aggcgtgaag gtcggctggt cagaagtggc gccgcaaaca tacgagcgct ggatgctgta
     4741 tgtgcaagac accggtcccg gttttgaaga cggttcggtt accccgctag cgcaagcgat
     4801 caaaaaatcc acggaagagg ttcaggtggt ggaccaggag gcagaaaagg ccggtcagga
     4861 ttcaaccttt atcgctcccg cgcccgtgct tccgtctgag tcaagacacc gaccggacta
     4921 tcaaccgccc ggcgaaggga tcggcttatc gatcgttaag aaactctgcg agctcctgga
     4981 cgccagcttg gaatttgaaa ccaagcgggg caaagggagc acgttccggg ttatattccc
     5041 gcgccgttat gaagcctagc gagcggcgct tccgaagttc accagtcata gttcacgcta
     5101 aatcataagc agaaatagct tacttgcgga ggcagcagtt gtctggcttg cgatttgaat
     5161 ctctttccgt agaatctttg attgccaaat aaaacgggct ttatctcgtt ctctctctta
     5221 agtcggcagt gcggatgttt gacagagatt attgattcat tagattcttt ccggcaaatc
     5281 tcaatccaaa acactctcca ccgtcaggga ggtttcatgc gcaccagaat agaaggggca
     5341 ttaccgattg ccgctgttgc ggtttttctc agtatcggct cgcctgccgg gagcgagacg
     5401 aaaaaaccga ctacacttgc ggagctagcg gcctacacgg gggctgaccg tgaacagctt
     5461 ctattggccg gagccagagt cgaagggaaa gtcgtttggt acacatcctt ggccggcgcc
     5521 tcgttcaaag aaccgatgaa agctttcgaa gcaaagtatc ccggcgtcaa ggtcgaagtt
     5581 tatagatctc agagtagtga cctgggaaag aggatcacaa ccgagaacca ggcgaagcga
     5641 ttctacatgg acgcgctcga aacaacgctg ccgcttcaaa agcttctgct cgaaggtaag
     5701 atgatgactc cttttacttc gcctcatctc agcaagtatc cggatatcgc aaagcgaaaa
     5761 gcaaatgatg ggctatactt ttgggcagtg gaccgcgagt cgtattcagg attggcaatc
     5821 aacaagacct ctatctcggc agcaattgca aagaactatg aagatctcct caggcccgag
     5881 ttcaagggca aaataggttt tgcaatttcc gagactggac cgagagctat cggggccatg
     5941 gtcaaggcaa aaggcgaaga gtttgtacga aaactaaggt cacaggaagt ttcattacac
     6001 gcgatttcag gccgcgccgt tgccgacctg gttgcctcgg gtgaattggg tgcatcgccg
     6061 acggttttcc gcagccatgc atcggagatt gccgccaacg gcgccccggt tacctggatt
     6121 cccatggatc tggtgcctac taatgccggt gccgtcagtc ttccaatcag cgcgccgcat
     6181 ccgcacgcag ccgttctcct gatagatttc atgttgagtc ccgaagtgca gaccatcttc
     6241 gagaagggtc aacaaggtag cccggctaaa gaatatggtt tcaaacgctg gtatcctgaa
     6301 gagggaatga attccgagca atacgataag gcgagcacgc agtgggagaa actgctgcgt
     6361 gagatcggcc gcaactaaat gaaactctca agtatcacgg aggagaatcg aaagtgctga
     6421 ccatcgatgc tgatgcccat gtagtggaga gcgagcgaac ctgggactat ctggaaggta
     6481 acgacaggcg ctatcggccg gtacctatta ccgtcgacat gcctttggaa aagagaaaga
     6541 acttctggat tatcggtggc cgcctgattg gcgggcgcga caatatcggc aaggacactt
     6601 ctcaagagtc ccgggagatg gccgacatcg aggcgcgact aagacacatg gacgaaatcg
     6661 gcacggggat tcaggtgctc taccccactc tatttctacg cccgtggacg gaccaaccgg
     6721 agatcgagct agcgctgagc cgctcgtata atcgctggct tgctgacatc tggcataaag
     6781 ccaaggatcg cttacgttgg gcggtgcatt tgcctttgat gacgatggac aaagcgctgg
     6841 acgagcttcg ctgggcgtct gggaacggcg cctgcgctgt cttcatgcgc ggattggaaa
     6901 ccacgcatag gttgcacgat ccctacttct ttcccttgta cgaggaagca agccgcctca
     6961 acatgcctat cggcattcac tcaggcgtgg gaaatttcgc tgtctctgaa gcttttggcg
     7021 ttgacccttt tcgcgaagcc aaactcggag tgattggggc ctttcacgcc atgatcatgc
     7081 gaggcatccc ggaacgtttc ccgggtctcc gcatcggcgc cattgaagtt tccgcgcaat
     7141 ggattcccta cgtggtacat gacataaacc tgcgtttgcc gaagtttctc ggcaaagagg
     7201 ccaatcctga tgtgctgcgc aacagcagcc agctgtttgt cgcctgccag acggatgatg
     7261 atctgcccta tgtgctgaag tattccggtg aaaacagcct catgatcggc tctgattacg
     7321 gccatgccga caacgccagc gagttgctgg ctttgaatcg acttaaagcc aaaggaaaga
     7381 tcgaccccca tgtaatcgat aaaattctct atgacaaccc agcgcggttc tacggcttgt
     7441 cgattaattg aggtggatag acattaagaa cgattcgcca cgataggatc acttcatcgg
     7501 gcgaaatttc ggtaaagtgt agccttcgtt tgcccccgta aaccacttca ccgccgtgcc
     7561 gatctttact taatccggcg ggcagccgat gacatttgct gatcaaacgc gacttcttcg
     7621 tcaaggtcca cccacgaacg ttgtaaggcg ccttgtcttc gtacgctgga tgcaggcgcg
     7681 gcaataggtg acgaacgaat acaccttccc tttaccgctg agctgaaccc attatagtcc
     7741 ggtgagaggc agtgcgggca cgaagccttg cggcggttac atgaactttg caaccagcgc
     7801 ggatctgagt gctgagctgt gctggacagc gtcacacgtc ctaagccgcg aacgcatgac
     7861 gcctgattca cccagccgaa cgctgattaa gcgttgctcc cttcggagcc tctttcttct
     7921 ttttgaaata ctctcgctta gcaagtcgag tcgcatgccg cttgcggcgt tgcaagttac
     7981 gtttcgcatt ctgtttcatc tcgtaccttc actttcgtct gtttttaaat tatgcgcatt
     8041 ttatcagttg cttctcaatg gcagtcccag tccttatcac cgagataatg taagcgaagc
     8101 ggtatacaaa aactgcggca accgatcgct ctgatgacac cttacaggat gtgctgaaga
     8161 ggctaggagc taactgacga ttagacaagc cgtaactgct catctgagat cggattgatg
     8221 caatccgagg aatcgaactc aggtttatta accagcggcg aaacatcatg tgattccatc
     8281 agctcggaag gaaatggcgc aagcacggcg gcaatatccg caggacgggt actgagccgc
     8341 ggatccaacc actgtctcgc ctgcaatgcg tcgaagatta ccggcattcg gttgtgaatg
     8401 cggcgcaaca gcgcattggg cgaagtcgta atgattgtga acgtgtgcag tatctcccct
     8461 tccacgtccc gccacacgtc ccaaagaccg gcaaacgcga acggctcttt tttcttaatg
     8521 tagaaccaaa ccggaacttt accacttccc tcccggcgcc attcgtagaa gccatcggcg
     8581 ggaatcaggc agcggcggct ctcgatcagc cggcggaaac tcggcttctc ggttaccgtc
     8641 tccgcgcgcg cattgatcat ccggttagca atggatcgat ccggcgccca tgacgggacc
     8701 aaaccccagc gcatgatctt gagttcgttc tttcccccgt tctgaatgat gacaggcact
     8761 tcttgcgaag gcgcaatatt aaaccgacgg ccaaaaaacg ggagatcctg ctgaagattg
     8821 taacgcactt tgatttcacg aaattcgaaa gaagcggtaa agcgtccaca cataatggcg
     8881 attatctata gcatgcatgg cgagatcttt catcaacgca ggagattgtt taacctctac
     8941 gcaggagtca ggtcatcaaa acgattggag cactcagatg gttacagcgg atagtaagtg
     9001 tccacaatgc ggcaatggca tcatgaaact gtttccggag actaaaccgc ttattccgga
     9061 gcccgagcag agcgagttgc gcgcccgctg caccgcttgc aattacgagg acacagtcac
     9121 gattcgatcc gacgactaga ataagagtgc acgtaaacca catcactcgt ggcgccccga
     9181 taagctgtag ccgttccgct gatgcagtct cagccggttt gggttaattc atattgggac
     9241 actaacaagc gcccccaccg ccgattgtgt c
//
LOCUS       DASUTG010000605         6372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_390660, whole genome shotgun sequence.
ACCESSION   DASUTG010000605 DASUTG010000000
VERSION     DASUTG010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390660"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VF089_13950"
     CDS             <1..1084
                     /locus_tag="VF089_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175116.1"
                     /translation="QSHSVGLRREAEGVRKMVFSHKSKTCGERGRTIQKRQWAGLFAI
                     VLAVTFGGAVVQAQPARIPRIGILIPSSVSFLSARVEAFRQRLREHGYVEGKNIFIEY
                     RYAEGKPERVPDLAAELVRLKVDVIVTVSPTATLAAKKASGTTPIVFASANDPVGTGL
                     VSSLARPGGNITGLSLMAPDLDGKRLELLKEAFPKVARVAFLWNPSGLRGNLPLTEME
                     AAAKPLQLKLLSLEVRSLDDFDSAFARAKREGAQALITIPTALITTQQRQVLDFSSKN
                     RLPAMYPASEFVEAGGLMSYAPSYTDLWRRAADFVDKILKGAKPADMPVEQPTKFEFI
                     VNLKAAKEIGLTIPNRVLERADQVIK"
     gene            complement(1526..1684)
                     /locus_tag="VF089_13955"
     CDS             complement(1526..1684)
                     /locus_tag="VF089_13955"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX6175117.1"
                     /translation="MKPNHEKVLKEALRLPTEARAALAGRLLESLDETVDEDVESAWT
                     EEIARRIA"
     gene            2618..2926
                     /locus_tag="VF089_13960"
     CDS             2618..2926
                     /locus_tag="VF089_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175118.1"
                     /translation="MRKRALGLLLGAMFFALCSSGQAQQSTRILRLGFLTGGSAAQTS
                     ARTDAFRHGLRELGYVEGKNIVIEYRYAEGRSERLPELATKLVQLKVDVIVTTSAGAA
                     "
     gene            2923..3600
                     /locus_tag="VF089_13965"
     CDS             2923..3600
                     /locus_tag="VF089_13965"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175119.1"
                     /translation="MAAKKASGKIPIVFAAANDPVGTGLVSSLARPGGNITGLSLMAP
                     DLDGKRLELLNEAFPKVARVAFLWESGGTRGNLPLTEMEAAAKALGLKLLSLEVQSLD
                     DFEGAFARAKKERAQALITTTGALINTQQRRVLDFAAKNRLPAIYHYTEFVEAGGLMS
                     YGPDNTDIWPRAADFVDKILKGTKPADMPVEQPTKFELMINLKTAKQIGLTIPPSVLA
                     RADKVIK"
     gene            3614..4447
                     /locus_tag="VF089_13970"
     CDS             3614..4447
                     /locus_tag="VF089_13970"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175120.1"
                     /translation="MKSVLLLIGFVLASIHFADAQQPMKVPRIGFLAATYPSAISDRL
                     KAFRQRLHELGHVEGKTILFEYRFAEGKYDRLPELAAELVRLNVDIIVVSGGRPTAAA
                     KNATATIPIVVGSAGDLLRAGLVASLAKPGGNITGSTVSSADVSGKRLELLRETVPTA
                     TRIAVLWHPSPGDRPEGITSDWDEVLETQTEARRIGVEVQSIEVKDPNRFESAYAMMT
                     KQQAKAVILIRGSFTEFHHKRLGELALKHRLPSMCEAGHWVSDDCLMSYGPDSLYAWR
                     R"
     gene            complement(4475..4963)
                     /locus_tag="VF089_13975"
     CDS             complement(4475..4963)
                     /locus_tag="VF089_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6175121.1"
                     /translation="MPIPERYNLGYNIGVGGAVVRDGELLLVRRASRHGRGNWQLPGG
                     FIEPDETIEQAVVREVQEEAGVTAEVEAVLALRSRYDPESGNGVYIILLLRPVSGEPT
                     ADNHEVDHAGYFTLEQIRGLNPLPPVNWEIAQRVLAPDRRLLYPKPLANLNGAQYTLF
                     VG"
     gene            complement(5183..>6372)
                     /locus_tag="VF089_13980"
     CDS             complement(5183..>6372)
                     /locus_tag="VF089_13980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175122.1"
                     /translation="AIVIIDCDSHLMPRDAFDGVDGHFGSRKPKLKFDEKGLYADVEF
                     PGDAFPVPGTSPLGAPGSGAMFRSLWDVAARMSDYDERLGIEKHFVLPQFSGWWSYVI
                     EPELAGRMARSHNEAVLRIMEKYPGRILGVALVALQDVAGSIRELVWAKERGFVTAVI
                     DKIFPVKDHPYGEPLGSRRELWPFFRKAEELDMPLYLHNVQHGHRLSNLLPFQRDGLY
                     VFAPQEGHMSLVSLFTSGLLDACPRLQFIYTEAGTAFIKPLVQRLDKILEAPPVDYDD
                     QEESPLGNGNLSKIGEKLRRARAILPASEFLEKNKQPASHYFKNNFFFTIETEEPELA
                     EAIDFLGAQRFLFATDYPHDDPGGRMKFQDVELLRKHPDISEEDKELIRWKNAVRLFH
                     LDS"
BASE COUNT         1436 a         1745 c         1826 g         1365 t
ORIGIN      
        1 acagagtcat tcggtaggct tgaggcgcga ggcggaaggc gtgagaaaga tggtctttag
       61 tcacaaatcg aaaacctgtg gtgagcgagg ccgaaccatc caaaaacgac aatgggcagg
      121 gctctttgca atcgtgctcg cggtgacgtt tggcggggcg gtggttcagg cgcagccggc
      181 gagaattccc cggataggaa ttctgatccc gtcctccgtg tccttcctct cggcccgggt
      241 cgaagcattt cgccaacggc tgcgcgagca tggctatgtc gaaggaaaaa atattttcat
      301 tgaataccga tatgcagaag ggaaacccga acgggtgcct gaccttgcag ccgaactggt
      361 ccgtctcaaa gttgacgtca tcgtcaccgt cagccccacc gctacattag ctgccaagaa
      421 ggcgagcggg accactccga tcgtattcgc ctctgctaac gatccagtag gaactgggct
      481 cgtttccagc ctggcgcgac caggcgggaa tatcaccggt cttagtctca tggcgccgga
      541 tctagatggc aaacggttgg agctgctcaa ggaagccttc cccaaggttg cccgggtggc
      601 tttcctctgg aatccgagtg gtttgagagg aaacctgcct ctcacagaga tggaagctgc
      661 ggccaagccg ttgcaactca aacttctgtc gcttgaggtg agaagccttg acgattttga
      721 cagcgcgttt gcgcgagcga aaagagaagg cgcccaggcg ctcattacga tcccaactgc
      781 acttatcact actcagcaac gccaagtgtt ggacttctcg tcaaagaacc gcttgccggc
      841 gatgtaccca gcgagtgagt tcgttgaggc cggcggcttg atgagttacg cgccgagcta
      901 tacggacttg tggcggcgcg ccgctgactt tgtggacaag atcctgaagg gcgcgaagcc
      961 cgccgacatg cctgtggagc agccgacgaa atttgaattc atcgtcaatc ttaaagcggc
     1021 gaaagagatt ggtctgacaa tcccgaatcg tgtgctcgag cgggcggatc aggtaatcaa
     1081 atgagaatag gcaaccggca agaggcggta ggcaacagcg aatcaagtca tcaagtgagc
     1141 accaataatc tcagtataaa tttagccgat ttcaaaccgc cgagacttcc aatagccagg
     1201 acggcggcgc ccatgggcca cagcgataac ctgaatggtg gactgggtga tccggtaaac
     1261 ttccgaggga aactcggagg agaaatctcc ttgtacccgg caaatgcatg gtccaccgct
     1321 caggaccgtt ctggatttgt ctgatggcgt gatcgagttc agagatgaaa gcgtcgattg
     1381 cgttcggcat accaatcgta ggccgctttt gcctcagcga tcgcgtcagg atgagcctaa
     1441 cgcttgggag cggccaacgg atcgaccgag gatctgtcgc cgagccacgg accacggaat
     1501 tgtctttatt ttaccctggt ctagatcatg cgatgcgtcg cgcaatctcc tcagtccacg
     1561 ccgattcgac atcctcatcg actgtttcat ccaagctttc cagcagacga cccgcaagcg
     1621 cggcgcgtgc ctcggtggga agacgcaacg cctctttcaa gactttctca tgattgggtt
     1681 tcatgcttgc agattaatcg cccggatggg ctttcgttaa tcgatttgca cgtgtcggta
     1741 aacggcattc tctgtcgaca accatctcag gacggcgaag cagatcggcg tcacgatccc
     1801 gcagagcgtg ctcgcgcgag cggatcgagt gattcggtaa gatgtttcga gttccgggtt
     1861 tcagcttttg agatggataa gacgaatcca aaatccgtga gtgcggcaaa gttgcaccct
     1921 atcgtgatgg gagttcaaga gcgtgagcaa gggcgcggtt aagctgggcg agtttgaatg
     1981 ccgacaatgt ggacacaaag tgagtgagct tacgcttgaa catcagtgta agaaaatcgc
     2041 agcggatggc ggaagttcgt ggcaatccat cgtccggtcc gagcacgacc tctgttgtga
     2101 gtccgaggac ttctccgtaa acgggggcgc agatcacggt agagacatct tcgtgctccg
     2161 cgacaaaggt tcgggatacg acaacgtaaa aacccggctt tgccccctcg ctcggcaacc
     2221 cgttcgcgcg tgcggtagat ctcgccgcgc ctcacgggcg cgcttgctcc gtgatgagta
     2281 cacgggcttg ccgagcgagg cgtttgcggt gtttgcgaaa cacttcgccg tccttggcct
     2341 cagtttcacg gcgtcgttgg cgttccgcat attggcgcaa agccgtgcga actagcgccg
     2401 cgcggcttcg gcgtcgcgag gaactttcgg tcagttcatc caagagcttc gaagtagctt
     2461 catcaatagc aatctttttc atgccaagga ttataccagt agatttagat tacgctcaag
     2521 aattgcgctc tgtccccccg gctcaggctc gaatgatggc gggcagagcg gagtggacaa
     2581 agagcagagc gcagaaagta aaaacggaag aaaactcatg agaaagagag ccttaggttt
     2641 gctccttggc gccatgtttt ttgcgctttg ctcatctggt caggcgcagc agtcaacaag
     2701 aatcctcagg cttggatttc taactggtgg ctccgctgcc caaacttcgg cgcgtaccga
     2761 tgcatttcgc catggtctgc gcgagcttgg gtacgtggaa ggaaaaaaca ttgtcattga
     2821 gtaccgatat gcagaaggga gaagcgaacg gctgcccgag cttgcaacca aattggtcca
     2881 gcttaaagtt gacgtcatcg tcaccaccag cgccggcgct gcatagctgc caagaaggcg
     2941 agcgggaaga ttcccatcgt attcgccgct gctaacgatc cagtaggaac tgggctcgtt
     3001 tccagcctgg cgcgaccagg cgggaatatc accggtctta gtctcatggc gccggatcta
     3061 gatggcaaac gattggaact gctcaacgaa gccttcccca aggttgcccg ggtggctttc
     3121 ctctgggaat cgggcggcac gaggggaaac ctgcccctca cagaaatgga ggctgcggcc
     3181 aaggcgttgg gactcaaact tctatcgctg gaggtgcaaa gccttgacga ttttgagggc
     3241 gcattcgcac gagcgaaaaa ggaacgcgcc caggcgctca ttacgaccac aggtgcactt
     3301 atcaatactc agcaacgccg agtgttggac ttcgcggcaa agaaccggtt gccggcgatc
     3361 tatcactaca ctgagtttgt cgaggccggc ggcttgatga gctacgggcc agacaatacg
     3421 gacatatggc cgcgcgccgc tgactttgtc gacaagatcc tgaaaggcac aaagcccgcc
     3481 gacatgcctg tggagcagcc gaccaagttc gagctgatga tcaatcttaa aacggcgaag
     3541 cagatcggac tgacaattcc gccgagcgtc cttgcccgtg ccgataaggt gatcaaatga
     3601 cggagcgtca ggcatgaaat ctgttctttt gctgattggc tttgtcttgg cgagtatcca
     3661 ttttgctgat gcacagcagc caatgaaagt cccgcggatc ggattcctag ctgctaccta
     3721 cccttctgct atctcggatc gcctcaaagc attccggcaa cgtttgcacg agcttgggca
     3781 cgtggagggg aagacgattt tattcgagta ccgctttgcg gagggaaagt acgaccggct
     3841 tcccgagctg gcggcggagc tggtccgtct caatgtagac atcattgttg tatcaggcgg
     3901 tagaccaact gcggctgcca agaacgcgac cgctacgatt ccgatagtcg tagggagcgc
     3961 tggcgatctt ttaagagccg ggctcgtcgc tagtcttgcc aaaccaggtg ggaacattac
     4021 cggatcgacc gtgagttctg cagacgtgag cggaaaacgg ttggagctat tgcgagaaac
     4081 agtgcccacg gcaacgcgca ttgccgtgct ctggcaccct agcccgggag ataggcccga
     4141 agggataact tcggactggg atgaagtcct agagacgcag actgaggccc gtcggatcgg
     4201 cgttgaagtt cagagcatag aggtaaaaga tcctaatcgg tttgagagcg cctatgccat
     4261 gatgactaag caacaagcta aggcagtaat tcttattcgg ggcagcttca ctgaattcca
     4321 ccacaaacgg ctcggggagc ttgcgctgaa gcaccggctg ccgtcgatgt gcgaagcggg
     4381 ccattgggtc agtgacgact gtcttatgag ctacggaccg gactccctct atgcgtggcg
     4441 cagataggta aaatccgcta ccttgagaac cggctcaacc gacgaacagc gtatactgcg
     4501 cgccgtttaa attggcgagg ggtttggggt ataacaagcg gcggtccggc gcgaggactc
     4561 gttgggcgat ctcccaattc accggcggca aaggatttaa gccccgaatt tgctccaggg
     4621 tgaagtagcc ggcgtgatcg acttcgtgat tgtcggcggt gggctcgccg ctcaccggcc
     4681 gcaataagag aatgatatag acgccgtttc ccgactccgg atcatagcga ctccgcaagg
     4741 ccaagaccgc ttcgacctcg gccgtcactc ccgcctcttc ctgtacttcg cggaccaccg
     4801 cctgctcgat ggtttcatcc ggttcgatga agccgcccgg cagttgccag ttgccgcggc
     4861 catgacgcga agcgcgccgc accagcaaca gttcgccgtc acgcaccacc gcgccgccga
     4921 caccgatgtt gtagcctaaa ttgtagcgct cgggtattgg cataacgtgc gtgatcctaa
     4981 ccgactgtat tcaatcgatg cgcgttattt cgccgcgctt ggcacgtcat cgtgcatatt
     5041 tactccgtca ttccccatgc ttttagcggg aatccaggcc agctttgact ggacccccga
     5101 tttaaacatt cgggggtgac gtcttcggga aaaattcacg tccctgtgtt ctgatatgcc
     5161 gcagcttgct gcacttattc aatcacgagt ccagatggaa caacctgacg gcgtttttcc
     5221 accgtatcag ctctttatct tcttccgaga tatcggggtg ttttctaagc agctccacat
     5281 cttggaactt catccttccg cccggatcgt catgcggata gtcggtggcg aagaggaagc
     5341 gctgagcgcc caggaaatcg atggcttcgg caagctccgg ctcttcggtc tcaatggtaa
     5401 agaaaaaatt atttttgaag taatggctcg ccggctgctt atttttttcc aggaactcgc
     5461 ttgcgggcag gatcgcgcgc gcccggcgaa gcttctcgcc tattttggac aaattgccgt
     5521 tgcccagagg cgattcttcc tgatcgtcat aatccaccgg cggcgcttcg agaattttat
     5581 cgagacgctg gaccagaggt ttgataaatg ccgtgcccgc ttcggtgtaa atgaactgca
     5641 atctcggaca ggcatccaac aaaccgctgg taaacagtga aaccaagctc atgtggcctt
     5701 cctgcggcgc gaatacgtaa agcccgtctc tctggaacgg cagcaggtta ctgaggcgat
     5761 ggccatgctg gacgttgtgc aggtagagcg gcatgtccag ctcctcggct tttcggaaga
     5821 acggccagag ctcgcgccgg ctgccgagag gctcgccata aggatgatct ttgacgggaa
     5881 aaatcttgtc gatgacagcg gtcacgaaac cgcgttcctt ggcccacacc aattcgcgaa
     5941 tggacccggc aacgtcctgc agcgccacca gcgcgacgcc gagaatcctg ccgggatatt
     6001 tttccatgat gcgcaacacc gcttcattgt gcgatcgggc catccggccg gccagttcag
     6061 gttcgatcac ataagaccac cagccggaaa actgcggcaa tacgaaatgc ttttcgatac
     6121 cgagacgctc gtcgtagtcg ctcatgcgcg ctgccacatc ccacaagctc ctgaacatag
     6181 cgccggagcc gggtgcgccg agcggactcg tccccggcac tggaaaggca tcgccgggaa
     6241 actcgacatc ggcgtacaga cccttctcgt cgaatttcaa cttcggtttg cgcgagccga
     6301 agtggccgtc aacaccgtca aaggcgtccc tgggcatcag atgactatcg caatcgatga
     6361 tcactatcgc tc
//
LOCUS       DASUTG010000606         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1875059, whole genome shotgun sequence.
ACCESSION   DASUTG010000606 DASUTG010000000
VERSION     DASUTG010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875059"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VF089_13985"
     CDS             <1..1421
                     /locus_tag="VF089_13985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6175123.1"
                     /translation="IRKKKNRDRRGVLIMPVGEHLIPKMAALLPFKLTGAQQRVLAEI
                     TENMESAEVMQRLVQGDVGAGKTMIAWFSSLRVIENGFQAVWMAPTELLAEQHFRNIQ
                     FYADKLAIKTALVTGSLTAKERHAALDEISKGGTQLVIGTHALIQEGVQVPRMALGVI
                     DEQHRFGVVQRLSLQRITGRTDTAISLTGQPHMLLMSATPIPRSLAMVLYGDMEISVL
                     DEMPPGRLPVKTKVFTEAQRKTVYSRVLEELRRGHQAFVVYPLVEASEQLQNVRDATQ
                     MADKMRQSVFKEFGVGLVHGRMASAERDQVMRAFRDGSIRVLVSTTVIEVGIDIPNAT
                     VMVIEHAERFGLSQLHQLRGRVGRGSAAAYCLLVNRSPRNPIAQQRLRVMERESDGFR
                     IAEADLALRGPGEFLGTRQSGLSDFRLANLARDSRLLIDARAEAQAWLQNDPELKSPE
                     SQEMRRILLQRWGHRLQLGAVG"
     gene            1769..2947
                     /gene="alaC"
                     /locus_tag="VF089_13990"
     CDS             1769..2947
                     /gene="alaC"
                     /locus_tag="VF089_13990"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEX6175124.1"
                     /translation="MEFQRIKRLPPYVFSIIDGMKMQARHRGEDIVDFGMGNPDLPTP
                     PHVVAKLIEAASKPQNHRYSVSRGIYKLRVAISDWYRRRYQVDIDPDAEAIVTIGAKE
                     GLSHLAWATIDPGDVVLCPSPTYPIHQYAVVLAGGDLRCIPLITSEDFFGHLEEAIKQ
                     TWPKPKMLIISFPHNPTTEVVELDFFERIVDFAKEYDLMVVHDLAYADLTFDGYRAPS
                     FLQVPGAKEVGVEFFSLSKSYNMPGWRVGFAVGNEDIIHALARIKSYLDYGIFQPVQI
                     AAIQALNGPQDCVEDIRSMYQKRRDCLIDGLARAGWPIRKPKGTMFVWAEIPQAAKKM
                     GSIEFAKFILQEAKVAVSPGIGFGQYGDDHVRFALIENEHRTKQAVRNIKRALGKLLD
                     "
     gene            2997..4385
                     /locus_tag="VF089_13995"
     CDS             2997..4385
                     /locus_tag="VF089_13995"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX6175125.1"
                     /translation="MTARRKIGVGLLGAGVVGEAIQDNLLGASQGRVGEDLELELRRV
                     YTRNPKTKTWFKHRPELFTAQAIDVIDDPTVEIVIEALGFQEPGDLSVFRDYILRAFE
                     KGKSVVTSDKAVLARYGAEIWAAAQASEQHLRFEACVGGGIPVIRSLTESFAIEEPEA
                     LFGIVNGTCNYILSQMEKSAKPYAEALKEAQQNGYAETNPTADVSGSDAEAKLMLLSS
                     VAFGLQLQPGTTWRKGIEDIRAIDFRYAGRTGSSTIKHLAVAKRIGSSVQVFVSPVLM
                     PRDNLLASIDGATNAICFNGAKSAGIPRHRDCDYVLVGPGAGGGPTAVAVLGDVCELA
                     RAKPGGFTGLPSLVQPGTLALQPEDDIDGAFYVRFIVKDRPGIVGDIGQTFGALGVNI
                     AEIWQLRHSEDELQALTRSYNLRENFKELLPFVITLERATLRQMRAALDSVRDRDYIV
                     VDPVWFPIWAAR"
     gene            4523..>4711
                     /locus_tag="VF089_14000"
     CDS             4523..>4711
                     /locus_tag="VF089_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEX6175126.1"
                     /translation="MKFIILQGDGMADYPLEVLDGKTPLQAARTPNMDWLAARGVFGL
                     AHVIPEGFPPGSDVGNMSI"
BASE COUNT         1059 a         1249 c         1360 g         1043 t
ORIGIN      
        1 cgatcagaaa gaagaagaat cgcgaccgcc gcggtgttct tatcatgcct gttggcgagc
       61 acctgatccc aaaaatggcg gcgctcctgc cgtttaagct gactggcgcc cagcaacgag
      121 tgttagcaga gataacagaa aatatggaat ctgccgaggt gatgcagcgt ctggttcaag
      181 gggacgtcgg agccggcaag actatgattg cctggttcag ttcgttgcgt gtcatcgaaa
      241 acggatttca agcggtttgg atggctccca ccgagctttt ggccgaacaa catttccgca
      301 acatccaatt ctatgcggac aaacttgcaa tcaaaaccgc gctggtgact ggttcactaa
      361 ccgccaaaga acgccacgct gctctcgatg agatttccaa aggaggtact cagttggtta
      421 tcggtaccca cgcgctcatt caagaaggcg tgcaggtgcc gcgcatggcg ttgggtgtca
      481 tcgacgaaca gcatcgtttc ggggtggttc agcgcttgtc gctgcaacgc attaccggcc
      541 gcactgatac tgccatttcg ctcacaggac agccgcacat gctgctgatg agcgcgacac
      601 cgattccgcg cagtctcgcg atggtgctct atggcgatat ggagatctcg gtgctcgacg
      661 aaatgccgcc cggccgcctg ccggtaaaga ccaaggtgtt tactgaggca caacgaaaaa
      721 cggtttattc gcgggtgctg gaagaactac gccgcggcca tcaagccttt gttgtttacc
      781 cgctggtaga agcctccgaa caacttcaga atgttcgtga tgccacgcag atggccgaca
      841 agatgcgcca gtccgtattt aaagagttcg gcgtcggcct ggtacatggt cgcatggcta
      901 gtgccgagcg agatcaggtg atgcgcgcgt ttcgcgacgg aagcatccgc gtcctggtat
      961 cgaccacggt tatcgaagtg ggcatcgaca ttccgaacgc cacggtaatg gtgatcgagc
     1021 acgctgaacg gttcggttta tcgcaactgc accagctccg cggccgggtt ggacgaggca
     1081 gcgcggcggc atactgtctc ctggtaaatc ggtcgccgag aaatccgatc gcgcagcaac
     1141 gcctgcgcgt gatggagcgc gaatcggacg gctttcggat cgccgaagcc gatttggcgc
     1201 tgcgcgggcc cggcgaattc ttgggcacac gccaatccgg tttgagcgat tttcgtctcg
     1261 ccaatctggc gcgcgatagc cgcttgttga tcgacgcacg agccgaagcg caggcttggc
     1321 tgcaaaatga tccagagctc aagagccccg agtcacaaga gatgcggcga attctgctcc
     1381 aacgttgggg tcaccgttta caattgggcg ccgtcggata agcgataata acagcgttta
     1441 cgatcaaggc gaggccgtcg agcgagctgc cacaagcgtg tgttctccac gacctggtat
     1501 gttccgagcg ccgtgaagct caaactaagc cctgacagtg tccgttccga tgccttcagg
     1561 ttttgggctg gcgatttgat cgtgcggtcg ccatgttgtt cattaaggaa taatcctttc
     1621 aaccaccaga tgaagggtta gattgccgga aattgcgtgt cttttgcgcg gcctcatgtc
     1681 agaattggtg tcgtctaaag ttgcgccaga atgcccgatg gcctataaag cagcgaaaga
     1741 cttttcacca gaaacgaggt agcgtcgagt ggaatttcag cgcatcaagc ggctgccacc
     1801 ctacgtgttt agcatcatcg atggcatgaa gatgcaggcg cgtcaccgtg gcgaagacat
     1861 tgttgatttc ggcatgggta accccgatct gccgaccccg ccccatgtgg tggcaaagct
     1921 catcgaggcg gcaagcaaac cgcagaacca ccgttattcg gtgtcccgcg gcatttacaa
     1981 actgcgcgtg gcaatctcgg attggtaccg gaggcgctat caggtcgata tcgatccgga
     2041 tgccgaagcg atcgtgacca tcggcgcgaa agaaggatta tcccatttag cttgggcgac
     2101 catcgatccc ggcgacgtgg tgttgtgtcc cagcccgact tatccgattc accaatatgc
     2161 cgtcgttctc gccggtggcg atctgcgttg cattccgctg atcaccagcg aggacttttt
     2221 cggccatctc gaggaagcca ttaagcagac ctggcccaag ccgaagatgt tgatcatcag
     2281 tttcccgcat aacccgacca ctgaagtggt cgaactcgat ttttttgagc gcatcgtcga
     2341 cttcgccaaa gagtatgacc ttatggttgt gcacgacctg gcttacgccg atctgacgtt
     2401 cgacggttac cgagcaccga gttttctgca ggtgcctggc gccaaagagg ttggggtaga
     2461 gttcttctcg ctttccaaaa gctacaacat gccggggtgg cgcgtcggct tcgcggtcgg
     2521 caacgaagac atcatccatg cgctggcgcg gatcaagagc tatctcgatt acggtatttt
     2581 tcagccggtg caaatcgccg ccatccaagc gttgaacgga ccacaggatt gtgtcgagga
     2641 cattcgctcg atgtatcaaa aacgccgcga ctgcttgatc gatggtctgg ctcgcgccgg
     2701 ttggccaatc agaaaaccga aaggcacgat gttcgtgtgg gccgaaattc ctcaagccgc
     2761 gaaaaaaatg ggttccatcg aattcgccaa atttattttg caagaagcga aagtggccgt
     2821 atcccccggg atcggattcg gacaatatgg cgacgatcac gtccgattcg ccttgatcga
     2881 gaacgagcat cgaaccaagc aggcggttcg caacatcaag cgcgctctcg gcaaattgtt
     2941 ggattagcgc gcttctaagc ggctcagtta aaactccgga ttctttccat atttccatga
     3001 ctgcacggcg taaaattggt gtcggtctgt tgggtgccgg tgtagtcggc gaagccattc
     3061 aggacaatct tctcggtgcg tcacagggca gagtcggcga ggatttggaa ctcgaactcc
     3121 gcagagtcta cacccgcaat cccaaaacga aaacatggtt caagcatcgc ccggagcttt
     3181 tcacagcgca agccatcgat gtcatcgatg atccaacagt ggaaatcgtc atagaagcgc
     3241 tgggatttca ggagcccggc gacctgtcgg ttttccgtga ctatattctg cgagctttcg
     3301 aaaagggaaa atcggtggtg acctccgata aagcggtgct ggcgcgctat ggcgcggaaa
     3361 tttgggcggc ggcgcaggcg agcgagcagc acttgcgttt tgaggcttgc gtcggcggcg
     3421 gcattccggt tatccgatcg ctcaccgaaa gctttgcgat cgaagaacct gaagcgctct
     3481 ttggcatcgt caacggcacc tgcaactaca tcctgtcgca aatggagaag agcgccaaac
     3541 cctatgccga agcgctcaag gaagcgcagc aaaacggcta tgcagaaacc aatcctacag
     3601 cggacgtaag cggcagcgac gccgaagcca agttaatgtt attgagctcc gtggctttcg
     3661 ggcttcaact gcagccgggt accacatggc gcaagggcat tgaagatata cgcgcgatcg
     3721 atttccggta tgccgggcga accggctcaa gcacaatcaa acatctggca gtggcaaagc
     3781 gaatcggttc atcggttcag gtgtttgtct ctccggtttt gatgccgcgc gataatctcc
     3841 tcgccagcat cgatggcgca accaacgcaa tctgctttaa cggcgcaaaa agcgccggta
     3901 tacctcggca tcgtgattgc gattatgtgc tggtcggtcc tggcgcgggc ggagggccca
     3961 cggctgtcgc cgttcttggc gatgtctgtg aattggcgcg agccaaaccg ggtgggttta
     4021 ccggcttgcc cagcctggtg cagccgggaa cccttgcgct gcagccggag gacgatattg
     4081 atggcgcttt ctatgttcgt tttatcgtca aagatcgacc ggggatcgtt ggcgatatcg
     4141 gccaaacgtt tggcgctctc ggtgtgaata tcgcggaaat ctggcagctc agacacagcg
     4201 aggacgagct gcaagcgttg acgcggagtt ataatttaag agaaaacttc aaggagcttc
     4261 tgccatttgt gataactctc gagcgagcga ccttgcgtca aatgcgtgcc gctctggatt
     4321 cggtgcgcga tcgagattat atcgtagttg atcctgtttg gtttccgatt tgggccgcca
     4381 ggtgaaaggt tcagcgacgg aggaacagac gtatagagct gttcgaatac gacgttattc
     4441 ttcaggctgg tctcggcatg caaatcgaac ttttttactg agcagaattc ccgccgggaa
     4501 tatctcaaaa tgaactagga agatgaaatt tatcattcta cagggcgatg gcatggcgga
     4561 ctatccgctc gaagtgcttg acggcaagac tcctctccaa gccgcccgaa caccgaatat
     4621 ggattggctt gcggcgcgcg gagtgtttgg cttagcccac gtaattccgg aaggttttcc
     4681 cccgggcagc gacgtcggca atatgagcat t
//
LOCUS       DASUTG010000607         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1278873, whole genome shotgun sequence.
ACCESSION   DASUTG010000607 DASUTG010000000
VERSION     DASUTG010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278873"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1126
                     /gene="prfB"
                     /locus_tag="VF089_14005"
     CDS             join(16..72,74..1126)
                     /gene="prfB"
                     /locus_tag="VF089_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_123290836.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6175127.1"
                     /translation="MLEETKEQLVQLEQRLDKLGGVFDVESKQKRLEELTQITSQPNF
                     WNDADKAQTIFKEQSSLKTVIENWEKHRTDLEEARFFLDLAGEEEGDEALQEAAAKVA
                     EVTQGMAETELAQILGGADDRRNAIVTLHPGAGGTEAQDWAEILLRMYLRWADRHGYR
                     KEILEYQPGEEAGLKSVTFSVEGDYAYGYLKAEAGIHRLVRISPFDANSRRHTSFASV
                     FVYPEVDDTIKVEVDEADLRVDTYRSSGAGGQHVNKTDSAVRLTHLPTGIVVACQNER
                     SQHKNRAMAMKILRSRLYELELEKQKEKMDTLHKTKKDNAWGSQIRSYVLHPYRLVKD
                     HRTNIEIGNADAVLDGDIDPFIQAYLLHDSDSPAA"
     gene            complement(1309..1653)
                     /locus_tag="VF089_14010"
                     /pseudo
     CDS             complement(1309..1653)
                     /locus_tag="VF089_14010"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1666..1800)
                     /locus_tag="VF089_14015"
     CDS             complement(1666..1800)
                     /locus_tag="VF089_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175128.1"
                     /translation="MAKQLNAGDFFPEYRVQTVDGRTLRLPDDLSGEYSALIFYRGGW
                     "
BASE COUNT          658 a          691 c          720 g          647 t
ORIGIN      
        1 aaagggcgag ggctgatgtt agaagaaacg aaagagcaat tggtgcaact ggaacaacga
       61 ctcgataaac ttcggaggcg tctttgacgt cgaaagcaaa cagaagcgcc tcgaggaact
      121 aactcaaatc acttcccagc cgaatttctg gaacgacgcc gacaaagctc agacgatatt
      181 caaagaacag tcctctctta aaactgtcat agaaaattgg gagaagcacc gaacggacct
      241 cgaagaggct cggttttttc ttgaccttgc cggtgaagaa gagggcgacg aggctttgca
      301 agaagcggca gccaaagtcg ctgaggtgac ccaggggatg gctgagaccg agctcgcgca
      361 gatcctcggc ggggccgatg atcggcgtaa tgccatcgtc acactccatc ccggagccgg
      421 tggcaccgag gctcaggatt gggcggaaat tttgctgcgc atgtatttgc gctgggccga
      481 tcgacatggc taccgcaagg aaattctgga ataccaaccc ggcgaagaag caggtttaaa
      541 gagcgtcacc ttcagcgtcg aaggcgacta tgcctacggc tacctgaaag cggaagccgg
      601 cattcaccgg ctggtgcgaa tctctccttt tgacgccaat tcgcggcgtc atacttcgtt
      661 tgcttcggtg ttcgtctatc cggaagtcga tgacacgatt aaggtggaag ttgacgaagc
      721 ggatttgcgt gtcgacacgt atcgatctag cggcgccggc ggacagcacg tcaataaaac
      781 ggattccgcc gtccgtctca cccatctccc taccgggatt gtcgtcgcgt gtcagaacga
      841 acgctcgcag cacaagaacc gcgcaatggc gatgaaaatt ctgcgctccc gtttgtacga
      901 attagagctc gagaagcaaa aagaaaagat ggatactttg cacaagacta agaaggataa
      961 cgcctggggc agccagatcc gctcctatgt gctgcatcct taccgtttag tcaaagatca
     1021 tcgtactaat atcgaaatcg gcaacgccga tgcggtgctc gacggcgata tcgatccatt
     1081 tattcaagcc tacttattgc acgacagtga ttcgccggcc gcttgagtct tcgcaccgag
     1141 acacgataat ctgattcacg gccgggataa tttttcagcc gttcgctaaa gtactggagc
     1201 ttgctcgccg ttagtagcca gcgcgttgct ttcgctccgg agggaccgcc ggtcgatcac
     1261 agggcgactt ttaattaatc cgcgtttata cgataggatt tgcatcgctt acttcggctg
     1321 tacctttctt ttcttgaaga actgaatatg acttagcgcg tcgacggcga cgatgcggcc
     1381 gatcggaccg atgctataac tagcgtcaac taccttttta tccgagtcca aaataaaatt
     1441 cgtggcatga atgattctcc gccgctcttc ccagaaggct cctatttttt ccgcatccgc
     1501 tgggacttca agcccgtagg ccacgggaaa cgtcaactgc aacttctcca cggtttctct
     1561 ggccttatct agtgagtcta cggaaagggc caagatctcg acattctctt tttcaaactc
     1621 atttatgtgc gattggaaat cggccaactg ccgaaggcag taaggtcacc agccgccacg
     1681 ataaaaaata agcgccgagt actctccgga taaatcgtct ggcagacgaa gcgtccggcc
     1741 gtcgacggtt tgaactcgat actctggaaa aaaatcgccg gcatttaatt gtttcgccat
     1801 atttcttcct gctctgcgcg atctagggtt ttgccgaagc gtggtcggtt gttaacttca
     1861 tatcatcctt tcagggtttt agtagcttct gcgcttgctg caaactcgcg aatcaacatt
     1921 tcgttccatt gattcaagtt ggcttagtgc ttccccgatt cagcttcagt aagtaacgga
     1981 agagatcgct gcggtaaagc ccaccgtagg cgatagcaat tctcttttgc agaagtagct
     2041 tcagcggcca tcgagtccga attgggcatc agatcaccgt ggtaaagaat ccgatcgaag
     2101 cgaagctgat taacaatcag caagctttgg gcgaaaggtt agcggcgact gggggccgac
     2161 cataaccgtg agcgtaggcc agcgggcgtc cctggagcat gcgaactcgt gtcatccgat
     2221 taactttcct tgccggaagt gtgtttcccc tccgctccag ttctattctt tcggtactcg
     2281 gcagtttgaa gtcgctcctt caggatcggg catcacaaga ttcccgcttg gattctagat
     2341 tgatggagtc cctcctgcgc tggttgtctc agatcggcgc aagtgataat gctcacgcct
     2401 aaaccgggcg aggttcaatt tgtcgggtag ctgaagcccc tgttccacgt tccgaataga
     2461 gagttcgcgg atctctgtca tgatttgaaa tagagcggca tggagaggat cgttcttctg
     2521 gcagggcgca tgttgactac ttataggttt attaatagta cgaggtcaaa tacagacggg
     2581 gttaacggct ctgaacggcg gagacagcga gcctttgatg atcaaagaga agcgatgatc
     2641 gatctctgtt actgacgacg ccgaatggac gcaagtcatt agaggttggg cggcgaccgc
     2701 gtttcagcgc gcctac
//
LOCUS       DASUTG010000608         6128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_390948, whole genome shotgun sequence.
ACCESSION   DASUTG010000608 DASUTG010000000
VERSION     DASUTG010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390948"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1188
                     /locus_tag="VF089_14020"
     CDS             223..1188
                     /locus_tag="VF089_14020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX6175129.1"
                     /translation="MTSDEKTLPIDLPLLNIDLSKQDDLEDADSREKALVPFDPLQRY
                     LAEIRRFPLLSREEEHKLAVEFKEFGNIEAAYKLVTGNLRLVVMIAREYQKALKSLLD
                     LIQEGNMGLMEAVKNFDPYRGVRFPSYAVWWIRAYIIRYIMNDWRMVKIGTTQAQRKL
                     FFNLQKEKERIEAEGLTPGPKLLAQRLNVKEDEVIEMEQRLANRDLSVDMPIGEDEEA
                     TMLNFLPDQKHNPEEHFAESQYRELLQQKMEDFARTLKDKELVIFRERLLNEDPITLR
                     EIGEKYNISRERVRQIEERLKKKLKAYLSKELDDVNGIRIGKTAD"
     gene            1301..1753
                     /locus_tag="VF089_14025"
     CDS             1301..1753
                     /locus_tag="VF089_14025"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6175130.1"
                     /translation="MQFEKQVEIQASREKVWNFIWDVDRFIACVPGCKEAKTIEEGKR
                     YSAIMVEKVGPFKVEFPTTIEVLERDELSRIKAQASGADNKIGSRMKLDLEVKLREED
                     GKTILQFVAGVDILGKLAALGHGIIKRKADQVLDEFAQAVKKRLEGDS"
     gene            1755..2621
                     /locus_tag="VF089_14030"
     CDS             1755..2621
                     /locus_tag="VF089_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6175131.1"
                     /translation="MLRRFRLEEPTSIAEVSELLGRFGDSAKIYAGGTELLLAMKEGF
                     VQFERLVNVKGVSGLNEVRENNGAVHIGALCTHRQLENSPILKEKLPALAKLEQNVAN
                     VRVRQVGTLGGNLCFAEPHADPGTLLIALEARLVAEQGDRKREIAAEDFFVDAYETAL
                     EPDEVLTEIIVPVPAQPAGVCYMKFGYLERPSVGLAVVVKLNSGKTQIDDIRIAVGCA
                     GPAPRRVAEAEALLRGKSLDESTRNIPQAGETSGRASQAISDLHGAQDYKEHIVQVLL
                     KRSFQSALSQCT"
     gene            2639..3160
                     /locus_tag="VF089_14035"
     CDS             2639..3160
                     /locus_tag="VF089_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6175132.1"
                     /translation="MAMTMLRSSNLQTLPVSFTLNGKLQELDVEPHELLLDVIRDRLG
                     LTGAKRSCDVQVCGACTLLVDGRPVSACTTLAFEVRGRSVMTIEGLAENGKLHPLQQA
                     FIEHGGFQCGFCTPGMILAAKALLDENPHPTEEELKHFMHGNICRCTGYKKIIESIMA
                     AAKNMSETTQTGS"
     gene            complement(3327..4250)
                     /locus_tag="VF089_14040"
     CDS             complement(3327..4250)
                     /locus_tag="VF089_14040"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6175133.1"
                     /translation="MGVVFWKELADHFSSRRFMILIFIIVLTGLWATYATGQAIRQNT
                     ESVPAQYVFLLLLTSSGETLFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSFINGKFFAGLTTIAVLWASILLLVIGLGITLLGFPPNAEELWRMLIFTVVGI
                     FYVGFWLALAMLFSLLFQKTVTAALGAIAVWLFMTLFVVIVSQGIASIVVPDTSTPEA
                     VARRADIENIIGRISPSTLFAETVHILLNPAARVFGLALQSQAEGILPTPLALGQSLN
                     LIWPHVTTLFGLVAICFGISYIFFMRAEIRA"
     gene            complement(4254..5186)
                     /locus_tag="VF089_14045"
     CDS             complement(4254..5186)
                     /locus_tag="VF089_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175134.1"
                     /translation="MSAVVDTKNLTKRYREKLAVNTLTLSVQQGEIFGFLGPNGAGKT
                     TTILMLLGLTEPTSGQLSVCGFNPTHEPLEVKKRVGYLPENPGFYDDISARENLLYMA
                     RLNRIPENEAQRRTAEVLEQVGLSEDGRRLVREFSRGMKQRLGIAEVLVKNPIAIILD
                     EPTLGIDPDGAIRILELIKNLNRDHNLTVMLSSHQLQQVQQICSRVGIIVKGRLIVQG
                     EMDELGRAILKGRQWNFLLEVGGNPDSLDRSLQSIDGIDEIEKRSHGWFLRCTKDVRP
                     EVVSLVTTKGLPLLQLRSEDPTLEEIYLKYFREA"
     gene            complement(5183..>6128)
                     /locus_tag="VF089_14050"
     CDS             complement(5183..>6128)
                     /locus_tag="VF089_14050"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX6175135.1"
                     /translation="SRYPSYPVRSVTVQGEKSTTIEFKGKVPQNTKPGNYDIKVVAKD
                     TEGRTSYSDTINFRVTSAKVATGGLRLTSQYPVLTAPSGQTLKFTIDLKNETNKPLPV
                     SLTAQTPPGWAVRFKPQFGDTQYSSILLKENGSETVAVEIDTPANAEAKEQPVTIVAR
                     SGAFEASADLKVNLKGTQDLKMGSLAGTLNTSVTAGTRTPVDFVVANAGTAPIRNLNF
                     ISKKPSDKWTVEFRPDKIDALNPGEVREIKMDILAPERTIAGDYLMTLTANSPEVNRS
                     VEFRVTVSTPTLWGWVGFGIVALVVLGLVIVFFRLGRR"
BASE COUNT         1579 a         1473 c         1648 g         1428 t
ORIGIN      
        1 ttcctgtttg cagccagacc agcgctggat aactattcag taagtcccga ccgctaggat
       61 tcccgtcttt gagattgcgg catcgaaggt cggaaaattg ggaaaacatt atcttaggac
      121 agtaaggtaa gttattcttc caccgaacgt tgctccctgt taagcgctga ttacccgatg
      181 cgattgacct ctctctttgc cgttgaggct taatagtcgc taatgacctc agacgaaaaa
      241 acactgccga tcgatctacc tcttctaaac atcgatctgt caaagcaaga cgatttggaa
      301 gacgctgatt cgagagaaaa agcgttagtc ccgttcgacc ctttgcaacg atatctcgca
      361 gagatccgcc gtttcccgct cctgtcacgc gaggaagaac ataagctggc cgtcgaattc
      421 aaggaattcg gcaacatcga agcggcgtat aagctggtca ccgggaacct gcgactggtc
      481 gtgatgatcg cgcgagaata ccaaaaagca ttgaaaagcc tgctcgatct gattcaagaa
      541 ggaaacatgg gtttgatgga agcggtaaaa aactttgacc cttaccgtgg cgtgcgcttc
      601 ccatcgtacg cggtgtggtg gattcgcgcc tacatcatcc gctacatcat gaatgactgg
      661 cgcatggtca agatcggaac gactcaggcg caacgtaaac tcttctttaa cttacaaaag
      721 gagaaggagc gaattgaggc cgaaggtctg acaccgggtc ctaaactgct ggcgcaacga
      781 ctcaacgtta aagaagacga agtgatagag atggagcaac ggctagctaa tcgcgacctt
      841 tcggtggata tgccgatcgg tgaagacgaa gaggcaacga tgcttaattt cttacctgat
      901 caaaagcaca atccggaaga gcacttcgcc gaaagtcagt accgtgagtt attgcaacag
      961 aagatggaag actttgcgcg caccttaaaa gataaagagc tcgttatttt tcgcgagcgg
     1021 ttgctcaatg aagacccgat tacactccgc gagatcggcg aaaagtacaa tattagtcga
     1081 gaacgcgtcc ggcaaatcga agagcggctt aaaaagaagc ttaaagcgta tttgagcaaa
     1141 gaactggacg atgttaacgg catcagaatc ggcaaaactg cagattagca gcggattgcc
     1201 taactcgtgg cgtcagtcat tccggcgcgc tcgcaggcgt gtgatcttgt ctgttcagct
     1261 gggttttggt accagaagta gattaactta ggaggaatca ttgcagttcg aaaaacaggt
     1321 agagattcag gcgtccagag aaaaggtttg gaacttcatc tgggatgtcg atcggtttat
     1381 cgcttgcgtt ccaggttgta aggaagctaa gacgattgaa gaaggaaagc ggtactccgc
     1441 aattatggtg gagaaagttg ggcccttcaa ggttgagttt ccgacaacca tagaagttct
     1501 cgaacgggac gagttgtcac gcatcaaggc tcaggcgtcc ggcgctgaca ataaaatcgg
     1561 cagccgaatg aagttggatc tggaagtgaa gcttcgtgaa gaagacggta agaccatact
     1621 gcaatttgtt gccggagtcg atatcctggg caaacttgcc gccttgggac atggcattat
     1681 caaacgcaaa gccgatcagg ttctggacga gtttgcacag gcagtgaaga agcgtttgga
     1741 aggagattcg tgagatgctg cgacgattcc gtctagaaga gccgacgagc atagctgagg
     1801 tatcagaact actggggcga ttcggggatt cggccaaaat ctatgcggga ggaacggagc
     1861 ttctcctcgc catgaaagaa gggtttgttc aatttgagcg acttgtcaat gtgaaaggcg
     1921 tcagcggtct aaatgaggtt agggaaaata atggcgcggt tcatatcggc gcactctgta
     1981 cgcatcgcca acttgaaaac tcgcccatat tgaaagaaaa gttgcctgct ctggcgaagc
     2041 tcgaacaaaa tgtggcaaat gtacgggtgc gccaagtcgg cacattaggt ggcaatcttt
     2101 gctttgccga acctcatgcc gatcctggaa cgcttctgat tgccttagaa gcgcgtctcg
     2161 ttgccgagca aggagatcgc aaacgtgaga tagcggcgga agatttcttc gttgatgctt
     2221 acgaaactgc gctagaaccg gatgaagtgc taacggagat tatcgttccc gtcccggcgc
     2281 aacctgccgg ggtttgctat atgaaatttg gttaccttga gagacctagt gtgggcctgg
     2341 cggttgtagt aaagctcaat agcggcaaaa ctcaaattga tgatatcaga attgcagttg
     2401 gctgcgccgg accggcgccc aggcgcgttg ccgaagccga agccttgttg agaggcaaaa
     2461 gcctcgacga gtcgacgcga aacattccgc aggcggggga gacatccggc cgggcttccc
     2521 aggcaatcag tgatttacat ggcgcgcagg attacaagga gcatattgtc caagtacttt
     2581 tgaaacgctc atttcaaagt gccctgagcc agtgcacatg aacttaggag acttttagat
     2641 ggcaatgacg atgttacgaa gttcaaattt acagacgcta cccgtttcgt ttaccctgaa
     2701 tggtaaattg caggaattag atgtcgagcc gcatgagctg cttttagacg ttatacggga
     2761 tcgtttggga cttactggag caaaacgttc gtgcgatgta caagtttgcg gggcttgcac
     2821 cttgctcgtg gacggaagac ccgtaagcgc atgcacaact ttagccttcg aagtcagagg
     2881 tagaagcgtc atgacgatcg aaggtttggc ggaaaatggc aagttacatc cactccagca
     2941 agcctttatt gagcatgggg gatttcaatg tggcttttgt actccaggaa tgattctcgc
     3001 agcaaaggct ttgttagatg agaatcctca tcccaccgaa gaagagctaa agcactttat
     3061 gcacggcaac atctgccgtt gcaccggata caaaaagatt atcgaatcca tcatggcggc
     3121 cgccaagaac atgtctgaga ccacgcagac gggttcctag ttcgcaactg ctcttgtgaa
     3181 cgagatcaca gagtacgctc cttcgctact ttgctccaac cgcacagtga attagcttaa
     3241 gagcggttcg ctgtgtagag aagcggagcc ttttacgact gggagctcct cccgcatgag
     3301 tgaaggtcat ggccaggata agcctgttag gcgcgaatct ccgcgcgcat gaagaagatg
     3361 taagatattc cgaagcagat tgccaccaac ccgaatagag tggtgacgtg aggccagatg
     3421 aggttcaaac tctgtccaag cgcaagcggt gtcggtaaaa tgccttctgc ctggctttgt
     3481 agggctaaac caaaaacacg agcagccgga ttcaacagga tatgaacggt ttccgcgaat
     3541 agggtgctag gtgagatacg accgatgatg ttctcgatgt cggctcgccg ggcaaccgct
     3601 tcaggagtgg aggtatctgg gacgacgata ctggctatgc cttggctgac gatgactacg
     3661 aacagggtca taaacagcca cacagcgatt gcccccagag ctgcggtcac ggtcttttgg
     3721 aagagcaatg aaaagagcat ggcaagcgcg agccagaacc cgacgtagaa gatcccgact
     3781 acggtaaaga tcagcatccg ccacagttcc tcagcattag gtggaaagcc aagcaaggtg
     3841 atacctaggc cgatcactaa caagaggatc gatgcccaga gaaccgcaat tgtcgtgagc
     3901 ccggcgaaga actttccgtt gataaaactg tcccggtaga tgggctgtga gagaactcgg
     3961 ctcaatgtgc cgcgtgcgta ttcaccactg atggagtcga atcccaaggt aataccgacc
     4021 aggggcccga aaaagcccag aaaagtcgcc agcgaaaaaa gcgtttcgcc gctagacgtt
     4081 agcaggagaa gaaagacata ctgcgccgga acgctttcag tattctgccg gatcgcttgg
     4141 ccggttgcat aggtcgccca gagtcccgtc agtacgatga taaaaatcaa gatcatgaag
     4201 cgccggctgc taaagtgatc tgccagctct ttccaaaaga ccacacccat tttttaagcc
     4261 tcgcgaaaat acttcaaata gatttcttcc agagtcggat cttcagatcg cagctgtaac
     4321 aaaggaagtc ctttggttgt gaccagggaa acgacttccg gccgtacgtc tttggtgcag
     4381 cgcaaaaacc agccgtggct gcgcttttcg atttcgtcga tgccatcgat agattgtagg
     4441 cttcggtcga ggctgtccgg attaccaccg acttcaagta aaaagttcca ctgccgaccc
     4501 ttgagaatcg ctcttccgag ctcatccatc tcaccttgta cgatgaggcg gcctttgacg
     4561 atgatgccaa cccgactaca gatttgctgg acttgctgca attggtgcga tgaaagcatg
     4621 acagtcaagt tgtgatcccg attgagattc ttgatcaatt cgagaatccg gatcgcgcca
     4681 tcggggtcga ttcccaacgt cggttcgtcc aaaatgatcg ctatcggatt ttttaccaga
     4741 acttcggcga tgccgagcct ttgcttcatt ccacgagaaa actcgcgaac caagcgccgg
     4801 ccgtcttcgc tcagcccaac ctgttccaaa acctcggcag ttcgacgttg ggcttcattt
     4861 tccggaatgc gattcaggcg cgccatatag agaagatttt ctctggcgct tatgtcgtca
     4921 tagaagcctg gattttccgg caggtaccca actcgctttt taacctccag aggttcatgt
     4981 gtggggttga acccgcacac cgataattgt cctgaagtgg gttcggtgag tcccaacagc
     5041 atcaggattg tggttgtctt tccggcaccg ttgggaccga gaaagccgaa aatttctccc
     5101 tgttggactg acagagtgag agtattaacg gccagctttt cacggtaccg cttggtcaaa
     5161 tttttggtat cgactactgc gctcatcgcc gtcctaaacg aaaaaacaca atcaccagcc
     5221 caaggactac taatgcaaca atgccaaatc ctacccagcc ccagagagtt ggggttgaga
     5281 ccgtcacccg aaactccacc gaacgattga cctccgggga gttagcggtc aaggtcatca
     5341 agtagtcacc ggcgatggtt cgctccggcg cgaggatatc catctttatc tcgcgtactt
     5401 cacctgggtt caaagcatcg attttgtcgg gtctaaactc caccgtccac ttatcgcttg
     5461 gcttcttgct gatgaagtta agattgcgga tgggcgccgt gccggcgttg gcaactacaa
     5521 aatcaaccgg ggtccgagtg ccggcggtta cggatgtgtt gagagtcccg gccagcgaac
     5581 ccattttcag gtcttgggtt cctttaagat ttaccttcag atccgcactg gcttcgaagg
     5641 ctccggatcg cgccacaatc gtcactggct gctctttagc ctcagcgttg gcgggtgtgt
     5701 cgatttcaac agcgacggtt tcggaaccat tttctttcaa aagaattgac gaatattgag
     5761 tatccccaaa ctgtggcttg aaacgaacgg cccacccggg tggtgtctgc gcggtgagtg
     5821 agaccggcaa cggcttgttg gtttcgtttt tcagatcgat cgtgaactta agagtctgtc
     5881 cggagggtgc ggtcagaacc ggatattgac tggtgagtct caatcccccg gtagcgactt
     5941 tagcggaggt tacacgaaag ttgattgtgt ccgagtacga ggtcctaccc tcggtatcct
     6001 tggcgacaac cttgatatcg taatttcccg gcttagtgtt ttgagggact ttgcccttga
     6061 attcaatggt tgtggatttt tctccctgga ccgtaacgga gcgaaccgga taactggggt
     6121 atctggaa
//
LOCUS       DASUTG010000609         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2171587, whole genome shotgun sequence.
ACCESSION   DASUTG010000609 DASUTG010000000
VERSION     DASUTG010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171587"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF089_14055"
     CDS             complement(<1..873)
                     /locus_tag="VF089_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175136.1"
                     /translation="MAVAIKPLIIVEDDSFLRLIDVILDPSAPGELFDAFAHFCAHDL
                     PDLRGWCDQVRARVHDLYPADVRLVRDCDELRAAIPGADALVVEALPVGAPEIAAAGG
                     TLRLVQRYGSMTRNIDEAACSRSGIKIKTLRRRANIATAEQALALMLALARQLNQNAN
                     LISIEQITAAGYTPTQYDRARTPNGNWARITGLKTLYGRQLGIIGLGEIGRELAIRAA
                     ALGMRITYHQRHRLTAAEEQRYQASYSPLRQLLENSDCVSLHLPRGAETRGIIGGSEL
                     STIKPGAMLINVSQP"
     gene            1055..1207
                     /locus_tag="VF089_14060"
     CDS             1055..1207
                     /locus_tag="VF089_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175137.1"
                     /translation="MTEETAEQKLARLEAENRALRQQMERKPGQLRLKVSEKAPYPFT
                     GSAGFR"
     gene            1409..2482
                     /locus_tag="VF089_14065"
     CDS             1409..2482
                     /locus_tag="VF089_14065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6175138.1"
                     /translation="MSTSSMPINLFDFEAIAKERLPKGEYDYIAGGATDEISVERNRR
                     AFESWALRPRVLRDVSALDLSTTVLGTKVNLPVLIAPCGGHKKAHPEGEFATYRAAAA
                     RGTIMTVSANSNSSFEELAKAASGHLWLQLYPFRDRSLTRSWLDRAKEAGYEAICVTL
                     DSQWPPKRERNIRNNYRRTRGVNYPAQPLAPATAPRPAGAEGGSDPAATWKDLERIKS
                     QTSLPVVAKGIMTGEDVELCAEIGLDGVIVSNHGGRHLDNTLATVEVLAEAVVAAKGK
                     LEILLDGGIRRGADVVKALALGAKAVFIGRPLFWGLAVNGEQGVVRVLDILREEMEIT
                     MAKCGRPTIASIDSSVMVKVPRL"
     gene            complement(2750..3670)
                     /locus_tag="VF089_14070"
     CDS             complement(2750..3670)
                     /locus_tag="VF089_14070"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6175139.1"
                     /translation="MNRALLIGTSGYSYPGPPPKGWVGAFYPDPKPKRFDELHYYSQI
                     FNTVEINSTFYRPPSIASTTAWAKKTSSDFTFALKLWQKFTHPVKIGRAKTDEEWQPP
                     TQGDVDQFRAGVDPIFEAGKLGALLLQYPTSFHCTPENIERIERTLQVFFDYPKVVEL
                     RHKSWDERSGEIKALLGANRASSVLIDEPKFSSSIRQGLETIGEIFYFRAHGRNAKAW
                     WRPKESWERYDYLYSRQEIKKIAERIERATSAPEIKKGFAFFNNHARANAAANAIMLS
                     QELGLRLKAMPGEAMIAKFPELTNVERSST"
     gene            complement(3894..>4239)
                     /locus_tag="VF089_14075"
     CDS             complement(3894..>4239)
                     /locus_tag="VF089_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175140.1"
                     /translation="KTMNLYLSRQGHTVEVAHTGREGLQAARRFQPDVILCDIGLPEF
                     DGYKVAGQLRQDPELNRVYLIGISGYGQEHDKERAWQAGFNAYLVKPVDMSELESLLA
                     KFQTDASICGMM"
BASE COUNT          972 a         1115 c         1199 g          953 t
ORIGIN      
        1 aggctgtgac acattgatga gcattgcgcc gggtttgata gtggaaagct cggagccccc
       61 gatgatgccg cgagtctccg caccacgcgg gagatgcagg ctcacgcagt cggagttctc
      121 gagaagttgc cgcagtggcg aatagcttgc ctggtaacgt tgttcttccg ccgccgtgag
      181 acgatggcgc tggtgataag tgatgcgcat gcctaacgcc gctgcccgta tcgcgagctc
      241 ccggccgatt tctcccaatc cgatgatgcc taattggcga ccgtaaaggg tcttcaatcc
      301 ggtgatgcgc gcccagttgc cgttgggcgt tcgcgcgcgg tcatactgcg tcggtgtata
      361 acccgctgcc gttatctgct cgatgctgat cagattggcg ttttgattga gctggcgcgc
      421 cagagcaagc atcagcgcca aggcttgttc ggcggtggcg atattagcgc gccgtcgaag
      481 ggtttttatt ttaatacccg agcggctgca tgctgcttca tcgatgttac gagtcatcga
      541 gccatatctc tgtaccagcc gcagggtacc gcccgctgcg gcaatctccg gcgcgcccac
      601 tggcaacgct tcgaccacga gcgcgtccgc tccaggaata gccgcccgta gttcgtcgca
      661 gtcgcggacc agccggacat ctgccggata caagtcatgt acacgggcgc ggacttggtc
      721 acaccaaccg cgaagatccg gcaaatcatg ggcgcaaaaa tgcgcaaagg cgtcgaatag
      781 ctcgccgggt gcacttgggt cgagaatgac atctatcagc cgtagaaaag aatcgtcctc
      841 gactatgatc aggggcttga ttgcaacagc catcaattgc ttccggtatg ttcaccaaac
      901 tctttaaaca accgagtggt gatttttgtg caggaaagag aatctcgatc tcgcgttctc
      961 tgccgctttg cgataagaaa ctgttttgag acaaagagca acgatcctgt gaacgcagga
     1021 cgaaaaaatc tccgtgaaaa gtatcggagg aattatgaca gaagaaacgg ctgagcagaa
     1081 gctggcgcga ctcgaggcgg agaatcgggc actgcgccaa cagatggagc gaaagcccgg
     1141 tcaattgcgg ctgaaggtca gcgaaaaggc gccctatccc tttacgggct cggcaggttt
     1201 ccggtgactc tttacaagga gcagtggggc aggcttcttg accatgccga ggagatccgg
     1261 tcctttctca aagacaatga ccaacagcta aaggctaaac aatagccgag taacccatca
     1321 ggttgcaaaa ttacgagacg gatcaagcct cgcacttggt ctctaagcga tcgtccgcga
     1381 agagcaaggt tcattattgg gggataagat gagcaccagt tcgatgccga taaacctttt
     1441 tgacttcgaa gccattgcca aagagcgcct gccgaaaggc gaatacgact acatcgccgg
     1501 cggcgccacc gacgagatca gcgttgaacg aaatcgccgc gccttcgagt cttgggcgct
     1561 gcgcccgcgg gtgttgcgcg atgtgagcgc gcttgacctt tcgaccacgg tgcttggaac
     1621 caaggtgaac ctgccggtgc tgatcgcgcc gtgcggcggg cataaaaagg cgcatccgga
     1681 gggcgagttc gcgacctacc gcgccgcggc ggcccggggc acgattatga cggtcagcgc
     1741 caattcaaac tcctcctttg aagaattggc caaagcggcc tcaggccatc tgtggctgca
     1801 gctctatcca tttcgcgatc gaagcctgac tagaagctgg ctcgatcgcg cgaaagaagc
     1861 gggctatgaa gcaatctgtg tcactctgga ttctcagtgg ccgcccaagc gggagcgaaa
     1921 tattcgcaac aattaccgaa gaacccgggg tgttaactat ccggcacaac cactagcgcc
     1981 ggccactgcc ccaagacctg ccggcgccga gggaggatcg gatccggcag ccacctggaa
     2041 agatctggaa cggatcaaat cgcaaaccag tctgccggtc gtcgccaaag gcatcatgac
     2101 aggcgaggac gtcgagttgt gcgccgaaat aggactcgat ggtgtgatcg tgtctaatca
     2161 cggcggccgc catctcgata atactctggc aacggtggaa gtcctcgccg aagcggttgt
     2221 tgccgccaaa gggaaattgg aaattcttct cgacggcggc atcaggcgag gcgccgatgt
     2281 ggtgaaagcc ctggctttag gagccaaggc ggtcttcatc ggccggccgc tattttgggg
     2341 tctcgcggtc aatggcgagc agggagtagt gcgagtgttg gatatacttc gggaagagat
     2401 ggaaattact atggctaaat gtggccggcc gacaattgcg agcatcgact ctagcgtgat
     2461 ggtcaaggta ccgcggctgt aacgccgtat taatgagcgc cgctggagga gtagaaaaaa
     2521 cggagattcc gttaacgaat cgaatctatc gacgacataa tattgcctgt taacctctct
     2581 gccttcactt cgtctaatct tttaacaccg atgctccagc gcaggaatct atcgcaaggt
     2641 gagacgcagc atgtttgaat tcttattgtt ggccgtcgtg ttgatctatg tggcgatctc
     2701 ttatcgaccc gctcgcaagc taaacaccga tcgcaggcat gcgcacgggt caagtagatg
     2761 accgctcaac attcgtaagt tcggggaatt tagcgatcat tgcttcaccc ggcattgctt
     2821 ttaggcggag tccgagttcc tgagagagca tgatggcatt agcagcggcg ttggcgcggg
     2881 cgtgattatt gaaaaatgca aacccctttt ttatctccgg cgcgcttgtc gcgcgctcga
     2941 ttcgttcggc gatcttttta atttcctgcc gggaatatag atagtcatac cgctcccacg
     3001 attccttcgg cctccaccag gccttagcgt tgcggccgtg agcgcggaag taaaaaattt
     3061 cgccaatggt ctcgagcccc tggcgtatgg aggatgaaaa tttaggctcg tcgatcagca
     3121 cgctgctagc tctattggct cccaacaggg ctttgatctc cccgcttcgc tcatcccaac
     3181 tcttatgccg gagctcgacc acttttggat aatcaaaaaa tacttgcagc gttctctcga
     3241 tacgctcgat gttttcgggc gtgcagtgaa agctcgttgg atattgcagc agaagcgctc
     3301 cgagcttacc cgcctcgaag atggggtcca caccggcgcg aaactgatcg acatcgccct
     3361 gcgtcggagg ctgccattct tcatcagttt ttgcgcgccc aatctttacc ggatgcgtaa
     3421 acttctgcca cagcttcaag gcaaaggtga agtcggacga agtttttttc gcccacgctg
     3481 tagtactcgc gatggaggga ggccggtaaa aggtggaatt gatttccact gtattaaata
     3541 tctgcgaata gtagtgaagt tcgtcgaagc gtttaggttt tggatcggga taaaacgcgc
     3601 cgacccaacc cttgggcggc ggtccagggt atgaataacc tgaggttccg attagaagag
     3661 cccgattcac gattctgact gtctcgttga cggagacgcc ccttttgtct tttttatact
     3721 gacggctctg ggtatggatt caataggggg agcaagagga aggctatagg caaggcttca
     3781 tgtgacattt actacgtcta gtatagaaac gaagatgaaa tttctcgcga ggaatcacaa
     3841 gattactgtt gataaacgaa ggctaaccgc ccatgcagcg cgttggatac acatcacatc
     3901 atgccgcata tactggcatc cgtttgaaat tttgccagca agctttcgag ttccgacata
     3961 tcaacgggct ttaccagata agcgttgaag ccggcctgcc aggcgcgctc cttatcgtgt
     4021 tcctggccgt agcctgaaat tccaatcaga tacacgcgat tgagttccgg gtcttggcgg
     4081 agctgcccgg cgactttata accgtcaaat tccggaagac cgatatcgca cagtatgacg
     4141 tcaggttgga atcgtcgtgc ggcttggaga ccttcgcgcc cggtgtgtgc cacttccaca
     4201 gtatggccct gacggctgag gtaaagattc attgtcttt
//
LOCUS       DASUTG010000610         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1576326, whole genome shotgun sequence.
ACCESSION   DASUTG010000610 DASUTG010000000
VERSION     DASUTG010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576326"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VF089_14080"
     CDS             complement(<1..727)
                     /locus_tag="VF089_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175141.1"
                     /translation="MTVREIMQVGVGVPDREKFESFARDILGFPVLRSQDGRISYVRT
                     DQYQHRIAANTALEPVLRYVSFDIGGSEQLAGWASKLTAAGIAFRYGSPAQCAERRVS
                     EFIEFKDPDGHALALAYGFEIDESSVRYTRDLKVLRLGHVLLTVKDTQRSHDFYTGIL
                     GFRLSDWVYIDDNIRLCFLRCNERHHSLAFAPCMPGKTPRLQHAMFEVESLDDVMRSY
                     HFLRIHKAPIGMGPGRHPNCQTVH"
     gene            complement(1235..2806)
                     /locus_tag="VF089_14085"
     CDS             complement(1235..2806)
                     /locus_tag="VF089_14085"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HEX6175142.1"
                     /translation="MISQRTTTELTRRQFLKKIGTSAIAGSAIGVLKFSFAGGAQPPD
                     VSRPDSFGRMFRLPPFAPPTNAVRDALLELGKAGGIMDADDELAAGPTELITDVNLNL
                     VNQNSSSHTAGVTFFGQFLDHDLTFDARSRLGFPTQPITSLNTRTSFFDLDSVYAGGP
                     IATPQLYDSIDSIRFKVESGGLFEDLPRNPTTKVAFIGDPRNDENLLIAGLHAAFLLF
                     HNRAVDRVRSQHPSISDDDAFAEARRLTTWHYQWLILHEFLPLIAGRPLIEDVLTRGQ
                     RFYHPLHDQDFIPVEWQMAYRFGHSMVRPSYRANMKGDNGLPFFGMIFDPKGQGSADP
                     VDLRGGARAPRRFIGWQTFFDFGGTFSADVRPNKLIDTKISTPLFHLPLGAIASGMPP
                     VSLMQRNLLRHLTWQLPSGQAIAREMGVPQLSSGDLAELQSISRSFVNSTPLFYYILK
                     EAQVIENGNRLGPVGGRIIAEVFLGLLQRDPQSYCKVQPNWLPTLPTQGGTGEGFRMI
                     DFLKFAGVDPTSRGQ"
     gene            complement(3314..>4077)
                     /locus_tag="VF089_14090"
     CDS             complement(3314..>4077)
                     /locus_tag="VF089_14090"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175143.1"
                     /translation="TNLQAVIGGDVQIMAGGVPETVLARAGGLDIKNFWAISNVMPFQ
                     IYGSPKMKSLKDSKGKKFAISRFGSLSEFLTRSALRQAGVDPKDVTMLQIGATPARFT
                     TLATGMVDATILWFPVTERAKAVGMNKLFDLKDLYPNWTNVGFVAREIWLAKEKEQTT
                     KFLRAYQRGVKHTRENRDDGIRALRKYVGLDAAEAGAGYDEYRDSFPLDGHILDAGIV
                     ATVEQEMETGRLKAKISANEMIDYSFINAIGKSTR"
BASE COUNT          952 a         1103 c         1116 g          906 t
ORIGIN      
        1 cgtgcacggt ctgacagttg gggtgcctgc caggtcccat gccaatcggc gccttatgaa
       61 tgcgcagaaa atgatacgat cgcatgacat cgtcgagcga ctccacttcg aacatggcgt
      121 gttgcaatct cggagtcttt cctggcatgc aaggcgcgaa agccaagcta tgatggcgct
      181 cattgcaccg taagaaacac agacgaatgt tgtcgtcgat ataaacccaa tcactcaggc
      241 gaaagcccaa gataccggtg tagaagtcat gcgagcgctg ggtgtctttt accgtcagca
      301 gcacgtgccc caaccgcagc accttcagat cgcgggtgta gcgcaccgaa gactcgtcga
      361 tttcaaaacc gtacgccaaa gctagggcat gtccatcggg atctttaaat tctatgaatt
      421 cgcttacccg tcgctcggcg cattgcgccg gtgaaccgta gcggaaagcg atgcctgctg
      481 cggtcagctt gctcgcccaa ccggcaagct gttcgctgcc accgatgtcg aaactcacat
      541 agcgcagaac cggttccaga gctgtgttag ctgcaattcg gtgttgatac tgatccgttc
      601 ggacatagct aatcctaccg tcctgtgatc tgagaaccgg aaagccaaga atatcgcgcg
      661 caaagctttc gaacttttcc cggtccggca caccgacgcc gacctgcata atttctcgga
      721 ctgtcatagg taaatttcct tggagagtat ccgcctttag agccgcctca tttgttaagg
      781 cagcgagtta accgggcctc tccgttgccg ccagaataac cgggaagtta tcgttcgcgc
      841 agattgctgt caagaatttc tgatgaagtc ctgatcgaaa acagaaaaga tcattgggtt
      901 cgggaagtgg gtcggagtgt gttcaaccgt cagaaggtgg atcttaacgt tgttgaagaa
      961 acgcaagctc ctggcagttg aaccggcggg ataaacccac ggacgcgata ccacgatctt
     1021 caggatcaat cgccatgtca cacccgtaag taggagcggc gactagatcg tatgcggagg
     1081 tcagattctg tctgagtaat ccgacccgta tgtcacggct tgttggcgtt tatatcgact
     1141 tcatcccgga agagttctgt aatgggaccg ccatgcgatc gacagatcac gcaaaccgcg
     1201 aagtagcggg cccactcagt gccagcggag tctgtcactg ccctcggctc gtcggatcga
     1261 caccggcaaa ctttagaaaa tcgatcatcc ggaagccctc cccggtgccg ccttgtgtcg
     1321 ggagcgtcgg aagccaattc ggttgaacct tgcagtacga ttgcggatcg cgttgcaaca
     1381 agccgagaaa cacctcggca atgatccggc cgcccaccgg accgaggcgg ttgccgtttt
     1441 caattacctg agcttctttc aagatgtagt aaaagagcgg cgttgaatta acaaacgacc
     1501 tagagatcga ttgcaactcc gctagatcgc cgctgctcaa ttgcggcacg cccatctccc
     1561 gggcgatcgc ctgaccggat ggtaactgcc aggtcaagtg ccgcaacaaa ttacgctgca
     1621 tcaaagatac cggcggcata ccgctcgcga tggctcctag cggcaggtga aacagcggcg
     1681 tggaaatttt ggtatctatc aatttattgg gtcgcacatc ggcgctgaat gtgccgccaa
     1741 aatcaaaaaa ggtttgccag ccgataaacc tgcgcggcgc cctcgcgcct ccgcgcagat
     1801 ccactgggtc cgcactccct tggcctttcg gatcgaagat cattccgaag aatggcagac
     1861 cgttgtcgcc cttcatgttg gcgcgatagg aaggccgcac catgctatga ccaaaacgat
     1921 acgccatctg ccactctact ggtatgaagt cttgatcgtg caatggatgg tagaaacgct
     1981 gtccacgggt tagcacgtcc tcgatcagcg gtcggccggc aatgagtggc aagaactcgt
     2041 gcaatatcag ccattgataa tgccacgtgg ttaatcgtcg cgcctcggca aaggcatcat
     2101 cgtccgaaat cgacgggtgt tgcgaacgca cccgatcaac agcccggttg tgaaatagca
     2161 gaaacgctgc gtgcagtccg gctatcagga gattctcgtc attccgcgga tcgccgataa
     2221 aagccacctt ggtggtcgga ttgcgcggca gatcttcaaa cagaccaccg ctttcgactt
     2281 tgaatctgat cgagtcaatg ctgtcgtaaa gctgcggtgt cgctatcggg ccacccgcgt
     2341 aaacggaatc caagtcaaag aaggacgtgc gagtatttaa cgatgtgatc ggctgagttg
     2401 gaaaacctag gcgtgagcgc gcgtcgaagg tcaaatcgtg atcgaggaac tggccgaaga
     2461 atgtcactcc cgcagtatgg ctggaactat tttggttgac caggttgagg ttaacgtcag
     2521 tgatcaactc agtaggaccc gccgccagct catcatcggc atccatgatg ccaccagcct
     2581 tgccgagctc cagcaaagcg tcgcgcacag cgtttgtcgg cggcgcaaat ggcggcaatc
     2641 ggaacatcct gccgaaacta tcaggccgcg agacatccgg cggctgcgca ccgccagcaa
     2701 aagaaaactt taaaaccccg attgccgaac cggcgatcgc actggttccg atttttttaa
     2761 gaaattgtct ccgagttagt tctgttgttg ttctttgtga gatcatgtcc cctccttggg
     2821 cagattgttc gatcgaagct atgttgatgg gaagctgaac aaagacctcc ctttcgcacc
     2881 ggcacacggg aagttatctt acgtcgttag attgcggcgt aagtgatgaa tgcggaaggc
     2941 ttcgttcaag tgtgagcaca ggagatcggt acactcgcag gtttgatgcc gcggaaaatc
     3001 cggcagaata cctataatca tcgagaactg atcgtgtcca acaatgacaa aagtgttgag
     3061 ttcgcttgct ttagcgttcg tccgagaaca gttgcgctgg actgaaatag ctcagacaag
     3121 attccgagac aaaactcgcg atgagagttc ttcacacgcg ttacacgaaa ggctggggct
     3181 aggcctcgac acgtcggcag cttgtgggca tgagaggttc ggaggcgctt agataaccag
     3241 caaccggtgg cggacggtgg gaacttcgct agaaatgctc gaatctgtgt tatcggacgt
     3301 ttgaaaataa cgatcaacga gtactttttc caatggcatt tatgaagctg tagtcaatca
     3361 tctcgttcgc agagatcttc gccttgagtc tcccggtctc catctcctgt tccacggtag
     3421 caacgatacc tgcgtcgagg atgtggccgt cgagcggaaa cgagtcgcgg tactcgtcgt
     3481 atccggcgcc agcctcggcg gcatccagcc cgacatactt tctgagcgct cgaatgccgt
     3541 cgtcgcggtt ctcgcgcgta tgcttcacgc cgcgttgata cgcgcggaga aatttggtgg
     3601 tctgctcttt ttccttggcg agccagatct cgcgggcaac gaaaccgacg ttggtccagt
     3661 tgggatagag atcctttagg tcaaagagtt tattcatgcc gaccgccttg gcgcgttcgg
     3721 tgaccgggaa ccatagtatg gtcgcatcaa ccatgccggt ggccaatgtg gtgaaacgcg
     3781 ccggagtggc gccgatctgc aacatcgtga catccttggg atccacgccg gcctggcgca
     3841 gcgccgaacg agtgagaaat tcagacaacg agccgaatcg gctgatcgcg aacttcttgc
     3901 ctttgctgtc tttcagcgat ttcatcttgg gactgccata gatttgaaac ggcatcacat
     3961 tggagatcgc ccagaaattc ttgatgtcca gtccgccggc tcgggcgagc acggtctccg
     4021 gcacgcctcc cgccatgatc tgcacgtctc cgccaatgac tgcctggaga ttggtag
//
LOCUS       DASUTG010000611         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_835637, whole genome shotgun sequence.
ACCESSION   DASUTG010000611 DASUTG010000000
VERSION     DASUTG010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835637"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..755
                     /locus_tag="VF089_14095"
     CDS             315..755
                     /locus_tag="VF089_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175144.1"
                     /translation="MKLSVSDTDLNKLASQAFVWPNAIRNVHFSVVPEGVRVTAVYQQ
                     FIGVPFQMLGQLSVSGGKVTGRIERMRAGFISLGFLKAYLLKHIAAAMNIAIRNGMFI
                     FDVDALLADKGWPVRLNLTSIRCTPGSLILESRAPDHIHEAAGQ"
     gene            893..1585
                     /locus_tag="VF089_14100"
     CDS             893..1585
                     /locus_tag="VF089_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX6175145.1"
                     /translation="MADVAANYREIAERIRQAAISTGRDPRDVRILAAAKAQDIKSIE
                     AAIASGVLLIGENYVQEASLKRKSLPDNVEWHMIGHLQRNKAKAAVEIFDVIESLDNA
                     ELARELDKEGRKRNVQVRAFIEINIGNEETKSGIAKSKVAELLRTIGQLDHVSIQGLM
                     TVPPFRENCEETRPYFRELRELRDELHNLKLPKVDLRELSMGMTHDYLVAVEEGATIV
                     RIGTALFGPRRT"
     gene            1595..1894
                     /locus_tag="VF089_14105"
     CDS             1595..1894
                     /locus_tag="VF089_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX6175146.1"
                     /translation="MFILANFIIATAQVLDFLLWAYQWILIARVVISWIDADPYNPIV
                     RFIYNLTEPVLERVRERLPLIAGGFDLSPLVVWLAIWFIKRFLVQSLYDLAYALR"
     gene            1912..2073
                     /locus_tag="VF089_14110"
     CDS             1912..2073
                     /locus_tag="VF089_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175147.1"
                     /translation="MKSVQAEILVLVLDYSYSRVGLAMSRRVGISTVALPLARGVPSE
                     VERKIVRPA"
     gene            2095..3513
                     /locus_tag="VF089_14115"
     CDS             2095..3513
                     /locus_tag="VF089_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6175148.1"
                     /translation="MKILLEGRWVDKANKIEVKNPFDNSVIDTVPKADASDVAKALAY
                     AERGAKVIAKLTGYERWKILRTAADLIAARNEELGQTISKEEGKIIAEGRGEASRAVE
                     TMMGSAEEAKRLHGETVPLDADPTGGKKLGFTLRVPCGVVAAIAPFNFPLNLVCHKVG
                     PALAAGNSVIVKPASDTPLSALKLTEILLEAGLPPEGIQCITGSGGEIGDTLVKDRRV
                     RKITFTGSREIGDQICRMAGIKKVTMELGSNSPLIIMPDADLDRVAAAVAMTGYGNAG
                     QTCISTQRVLTAKKVYGDFLDALKPKVESLRTGNQLDEKSKVGPMVKESEAIRVEDWI
                     KEAVAGGARLAAGGGRRGAIYMPAVVADVTPDMRISRDELFGPAVAVTPFENIEEAIA
                     LANDSIYGLAAGIFTENVEWAMKFAREAEAGNLHVNWGPQWRVDLMPYGGLKDSGFGK
                     EGPRYAVEEMTELKMVVFHLNS"
     gene            3582..4304
                     /gene="rph"
                     /locus_tag="VF089_14120"
     CDS             3582..4304
                     /gene="rph"
                     /locus_tag="VF089_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX6175149.1"
                     /translation="MRSDGRKPKQLRLIKITPNYIKTADGSVLIEMGDTKVICTAKLE
                     ERVPPFLRNSGKGWITAEYGMLPGSSKARIGRESSRGKVGGRTHEIQRLIGRSLRAIT
                     DLANLGERTVWIDCDVIQADGGTRTASITGAYVSLCEAIRGWQKLGVIAQEPIKDSVA
                     AVSIGIVDGQILLDLCYEEDSKADVDMNFVMTGSGKFVEIQGTAESSPFTKKQMDKMS
                     DIAQQGIKELLNAQKQALAFTS"
     gene            4319..4939
                     /locus_tag="VF089_14125"
     CDS             4319..4939
                     /locus_tag="VF089_14125"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HEX6175150.1"
                     /translation="MIDLLVATTNSGKFAEIEAFLRTLPVDIFSLNDLQDPPVVAEDG
                     RSFEENALKKARTLADWSGYRTLADDSGLEVDALNGKPGILSARFSGAGADDAANNRK
                     LLEKLTEVPKEKRTARFVCVLALCAPRSHGGKQCTVRASCEGLIGFAAKGKNGFGYDP
                     LFFFPPLNKTFGEMDQQTKINVSHRGKALKRLVEVLPAWFDAAEKP"
     gene            4980..5056
                     /locus_tag="VF089_14130"
     tRNA            4980..5056
                     /locus_tag="VF089_14130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5014..5016,aa:Pro,seq:tgg)
     gene            5124..5200
                     /locus_tag="VF089_14135"
     tRNA            5124..5200
                     /locus_tag="VF089_14135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5158..5160,aa:Arg,seq:tct)
     gene            5214..5289
                     /locus_tag="VF089_14140"
     tRNA            5214..5289
                     /locus_tag="VF089_14140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5247..5249,aa:His,seq:gtg)
BASE COUNT         1308 a         1271 c         1530 g         1244 t
ORIGIN      
        1 cccgaagatt gcacgaaaaa atcagcaagc tgccatcata ctgtttcagg taaaagacat
       61 ccgtggtttc ctttagcctg cagctgctac atgcgacgac tcagcgaaga actttccttt
      121 gcctgatctg cgagtcactc aaaaaaactg tcggttgacg agcagcctgt ggtgaaacca
      181 cgaaacggac aagcggctga gctaggtctc ttctggcggg ttctcgaggc ttgcatctct
      241 atgtgcagcg agatgcgatt agatctgtaa attgcaagtg cgactggtat tttagcatca
      301 tggagataca ggtattgaag cttagcgtca gcgatacaga tctaaacaaa ttggcatctc
      361 aagctttcgt ctggcccaac gctatccgca acgttcattt ctcggtcgtg ccggaaggcg
      421 ttcgggttac cgcggtttat caacagttca tcggagttcc attccagatg ctcgggcaac
      481 tatcagtctc cggaggcaag gttaccggcc gtattgaacg gatgagagca ggattcataa
      541 gcctcggctt tctaaaagcg tatcttctga agcatatcgc tgctgcaatg aatatcgcta
      601 tccgtaacgg gatgttcata tttgacgttg atgccctgct tgcggataaa ggctggccgg
      661 tgcggctcaa cctgacttca atcaggtgta ctcccggtag tttgatcctc gagagccgtg
      721 cgcctgatca catccacgaa gccgcaggtc aataaggatc cgaatgaaag atttgttgga
      781 ctcaaatcga aatgagagga gtagcaaatt tggtttccga cggctccttt gattcccacg
      841 ctgtgttggc cggagtgatt tacaaaagcc gatatgataa gcaatcggca ctatggcgga
      901 tgtggcggcg aactatcgag agatcgctga acggatcagg caagcggcga tttcgacggg
      961 acgggacccg cgcgatgtta gaatactcgc ggccgccaaa gcacaagaca tcaaatcaat
     1021 cgaagctgcc attgcctctg gggtgttgct catcggggaa aattatgttc aggaggcgag
     1081 cctcaaaaga aagagtctcc ccgataacgt cgaatggcac atgattggtc acttgcaacg
     1141 caacaaggca aaagccgcag tggaaatttt cgacgtcatt gagtctcttg ataacgctga
     1201 attggctcgt gagctcgata aggaaggccg aaaaagaaat gttcaagtgc gggccttcat
     1261 tgaaatcaac ataggcaatg aagaaacaaa gtcagggatt gccaagtcca aagtcgcgga
     1321 gcttttacgc accatcggcc agctcgacca tgtcagcatt cagggactta tgactgttcc
     1381 gccttttcgc gagaactgtg aagagacgcg gccttatttt cgtgaactgc gcgagctgcg
     1441 cgatgaactg cataacttga agttgccgaa agtcgacctt agagagctat cgatgggcat
     1501 gacgcacgac tatctggttg ctgttgaaga aggcgcaacc attgtgcgaa tcggtacagc
     1561 cctgtttggt ccgagaagaa cgtagcttca ttagatgttt attcttgcta attttatcat
     1621 tgccacggcc caggtgttgg acttcctgct ctgggcttac cagtggatac tgattgcgcg
     1681 agtggtgata tcctggattg atgccgatcc ctataatcct atagttcgat tcatttacaa
     1741 cttgacagag ccggtgctcg agagggttcg tgaaaggttg cctctaattg ccggcggatt
     1801 tgatctttcg cccttggttg tttggctggc catctggttt atcaagcgat ttctggttca
     1861 atcgctttat gaccttgctt atgcgttgag gtgatcgccc ggcatgggtt gatgaaatct
     1921 gtgcaggccg agatactggt tttggtgctc gactactcct atagccgcgt tggtctcgca
     1981 atgtcacggc gggttggaat ttctaccgta gcgctgccat tagcacgtgg agtgccgtct
     2041 gaagtcgaga gaaaaatagt tcgccctgcg taattcgata aggagcttgt tattatgaag
     2101 attctcttag aagggcgttg ggttgataag gccaacaaga tcgaggtcaa aaaccctttc
     2161 gataattccg tcatcgacac ggttcccaaa gcggatgcaa gcgatgtggc aaaggcgctg
     2221 gcttatgccg aacgaggagc caaggtaatt gcaaaactta ccggctatga gcgctggaaa
     2281 attttacgca ctgcggcaga tctgatcgcg gcgcgcaacg aagagctcgg ccagaccata
     2341 tctaaagaag aaggaaagat tatcgccgag ggccgtggag aagcaagccg ggcggtggaa
     2401 accatgatgg ggtctgccga ggaggccaag cgcctgcacg gtgaaaccgt gccgctcgac
     2461 gccgatccta ccggtggtaa gaagctagga tttacattac gggtgccctg cggtgtcgtg
     2521 gcagctatcg cgccgtttaa ctttccattg aacctcgtct gtcacaaagt cggtccggcg
     2581 ctcgcggcgg gaaattcggt aatcgttaaa ccggcttcgg atacaccgct atccgcgctg
     2641 aagctcacgg aaattctttt agaagccgga ctccctccgg aagggattca atgcattaca
     2701 gggtcgggcg gtgagatcgg tgacactctg gtgaaagacc gccgcgtgcg aaaaatcaca
     2761 ttcaccggta gccgcgaaat cggcgatcaa atctgtcgaa tggcgggaat taaaaaagtt
     2821 acaatggagc tcggttccaa cagtccgctt ataataatgc ctgatgccga cttagacaga
     2881 gtggcggccg cggtggcgat gactggttac ggcaatgccg ggcaaacctg catctcgacc
     2941 caaagggtgc ttacagcaaa gaaagtctat ggcgacttcc tcgatgcttt gaaacccaaa
     3001 gttgaatctc tcagaaccgg taatcaactc gatgagaaat caaaagtggg cccgatggta
     3061 aaggagagcg aggccatccg cgttgaagat tggatcaaag aagcggttgc cggtggcgcg
     3121 cgcttggcgg ccgggggtgg ccggcgcggc gcgatctaca tgccagctgt cgtcgccgat
     3181 gttacgccgg atatgcgcat ctcgcgcgat gagcttttcg gcccggcggt cgcggtgact
     3241 ccgtttgaaa acattgagga ggccattgcg cttgccaatg atagtattta cggtctggcg
     3301 gccggcatct ttaccgaaaa tgtggaatgg gccatgaaat tcgcccggga agctgaagcc
     3361 ggcaatctgc acgtcaactg gggaccgcaa tggcgcgtcg atctgatgcc gtatggcggg
     3421 cttaaggatt caggctttgg caaagaaggg ccaagatacg cggtggaaga gatgacggag
     3481 ctcaagatgg tagtatttca cctgaatagt tgatggtagg atccaaacag ctttgacagc
     3541 gagcgaacaa cggagttgga cagcaagcat cggaagcaat gatgagaagc gacgggcgaa
     3601 aaccaaaaca actgcgactg ataaaaataa ccccgaatta cataaaaact gccgatggtt
     3661 cggtgctgat cgagatgggc gacacgaagg taatttgcac tgcgaaattg gaagagcgcg
     3721 tgccgccttt cctgcgcaat agcggcaagg ggtggatcac tgccgaatac ggcatgttgc
     3781 cgggctcatc aaaggcgcgc atcggccgtg aatcttcccg ggggaaagtc ggcggcagaa
     3841 ctcacgagat tcaacgtttg atcggccgca gtttgcgggc aatcacggat ttggccaatc
     3901 tgggtgagcg aacggtctgg atcgattgcg atgtgatcca ggcggacggt ggcacccgca
     3961 ctgcttcgat caccggcgct tatgtctctc tatgcgaagc gattcgcggt tggcaaaagc
     4021 ttggcgtcat cgcgcaggag cccatcaaag attccgttgc cgcggtgagc atcggaatcg
     4081 tagatggcca gatcctgcta gatctatgtt acgaggagga cagtaaagcg gatgtcgata
     4141 tgaatttcgt gatgacagga tccggtaagt tcgtcgaaat ccaaggtacc gcggagagct
     4201 ccccttttac caagaaacag atggacaaga tgtcagacat cgcccaacag ggaatcaaag
     4261 aactgctgaa cgcccagaaa caagctctcg cgttcacttc ctgaagtgtt tcgtctcgat
     4321 gatcgatttg cttgtcgcga caaccaacag cgggaaattc gccgagatcg aggcctttct
     4381 gagaaccctg ccggttgata ttttttcatt aaacgatcta caggatccgc cggtggttgc
     4441 tgaagatggt cggtcgttcg aagagaatgc tctcaaaaag gcgcgaacgc ttgcagattg
     4501 gtccggctat cgcacactgg ctgatgattc gggattagaa gtggatgctc tcaacggtaa
     4561 gcccggtatc ctgtcggcgc gatttagcgg cgcaggggct gatgacgctg ccaataatcg
     4621 caagctcctc gagaaattga ctgaagtccc caaggagaaa cgtaccgcgc ggtttgtttg
     4681 cgttttggcc ttgtgcgccc cgagatcgca cggcggtaaa cagtgcaccg tccgcgctag
     4741 ttgcgaagga ttgatcgggt tcgctgcgaa aggaaagaac ggcttcggtt atgatccgtt
     4801 atttttcttc ccgccattga acaagacctt cggcgaaatg gatcagcaga ccaagataaa
     4861 cgtcagtcat cggggcaagg cgctgaaaag gttggtagag gttctgccgg catggttcga
     4921 tgcggctgaa aaaccttgat ttagttcttt agccatgtag tattgggatg ttcctatgac
     4981 ggggcgtagc gcagcctggt agcgcacctg ccttgggcgc agggggtcgg aagttcaaat
     5041 cttctcgccc cgaccatcaa gactgctgag taatgagtgt tgagtcaggt taactcatcg
     5101 cttattactc gctcttgttt ttggcgccag tagctcagtt ggatagagca acggccttct
     5161 aagccgtagg tcaggggttc gaatcccttc tggcgcgcca gtatgggtat atggtgagtg
     5221 tagctcaacg gttagagcac tggactgtgg ctccaggggt tgagggttca aatcccttca
     5281 ctcaccccag gttttttgtc gaggatagag gaccgagaat cgacgatcgc tatcttccat
     5341 cctctatctt cga
//
LOCUS       DASUTG010000612         7015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_539201, whole genome shotgun sequence.
ACCESSION   DASUTG010000612 DASUTG010000000
VERSION     DASUTG010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539201"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1484
                     /locus_tag="VF089_14145"
     CDS             330..1484
                     /locus_tag="VF089_14145"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6175151.1"
                     /translation="MLPPRQATVSDLWHRQEYIVHHVRSGDTLPKILDRFRLTPGDKE
                     LWGRSFRQHFRSGKLAPGRQVHFYFAMPDVDRNRGGESLMAVEVDFNDSMDLTWEKTG
                     RNFFFARREKPVNVELRTATATIDGSLFTSGLKAGIHSSLLSQLADIFTWDVDLEKDI
                     YQGDSFKILYEQRSTRGQPNKPQLRILAAELVNAGEKKTAIYFEKQKGMGAYYNIEGR
                     SLARSFLRFPLEFANITSRFSESRFHPVLKTSLPHTGVDFAAERGTPVRAVGDGIINQ
                     VGWSGSYGKLVEIQHDASFTTRYAHLSGYGNGIRRGTFVKKGQVIGFVGATGRTTGPH
                     LHFELYKDQQYINPLSLDFPAEDTIEPALLTLFEDQKNTFLVELTSLPQS"
     gene            1755..2996
                     /locus_tag="VF089_14150"
     CDS             1755..2996
                     /locus_tag="VF089_14150"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX6175152.1"
                     /translation="MIQKVVILSTGDELTSGRVIDTNSAEIAERLFAIGIEVPAILKV
                     GDNKDQLLWALRQAQALGDSVIGTGGLGPTSDDLTSEVVAEFLGRKLRQNEHVARELQ
                     QRFKERGITWSANTLKQALFPEGSTIISNPVGTAPGFHVPIPPDKTLLWLSGVPQEMV
                     AMLDQTVLPWIERQRGEAAHASSCTFKIHGVTEGKLDEMLRPVQLGNNARLSFRAHYP
                     DLSLRLTVRTQRADGSLLFETIQKQIRALLGKHIYAEGNTSLEEVIGGLLMEKQQTLA
                     LAESCTGGYISHRVTRIAGSSAYYYGGAVTYSNEAKIQFLGVRPETLQEHGAVSRETA
                     LEMSAGIKQRTGADVGLSVTGVAGPTGGSAAKPVGTVWVSIAQNVRHEAKLFRFSGDR
                     EHVILGTSQVALNWLRTFLLR"
     gene            3003..3566
                     /gene="thpR"
                     /locus_tag="VF089_14155"
     CDS             3003..3566
                     /gene="thpR"
                     /locus_tag="VF089_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX6175153.1"
                     /translation="MIRAFIGVQIAPQVRARISEAIAHLAPELSGIRWVREENFHFTL
                     KFLGVIEEPQIEIISDALARAIQPFRRFTISAKSFGAFPEFKGARVLWVGLEGNAMPA
                     LAKTVESVLEPFGFPAENRIFRPHLTIGRWRHPMGVQQQLEGKLKRWKNFEFGEFSVD
                     EVVLFRSVLRPDGAVYRPLKAVPLAGE"
     gene            3605..4657
                     /gene="recA"
                     /locus_tag="VF089_14160"
     CDS             3605..4657
                     /gene="recA"
                     /locus_tag="VF089_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX6175154.1"
                     /translation="MDVNREKALDLAVSQIEKQFGKGAIMKLGEGGVTKDVPVISTGS
                     LGLDIALGIGGVPRGRVIEIYGPESSGKTTLALQIVAEAQKQGGMGAYVDAEHALDLG
                     YAKKLGVKTDDLLVSQPDHGEQALEIAETLVRSGAIDVLVIDSVAALVPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGTIARSHTVVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRIGALKDGDNIVGGRTRVKVVKNKMAPPFKEAEFDVLYGTGISRDGEIVD
                     LGSEMGIVEKSGAWYSFGGDRIGQGREAAKNFLKEHPEIAQQIMSKVMERVGLKREEA
                     PVTAINTDRKEKAKVK"
     gene            4684..5175
                     /locus_tag="VF089_14165"
     CDS             4684..5175
                     /locus_tag="VF089_14165"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX6175155.1"
                     /translation="MHKPKSPDSEEEALQSAFRYLSYRARSEAELLTHLCRKGYSAAA
                     CQKALEKLRTLNYINDESFARSWVWSRVAGRNYGPRRIEQELRAKGVEQPLIRDVIGA
                     IFACGDEEKNAKSLLEKRFKGVDLGDPKVLRRASAFLARRGYSSEVIFSLMRRRPDDD
                     LEP"
     gene            5172..>7015
                     /gene="alaS"
                     /locus_tag="VF089_14170"
     CDS             5172..>7015
                     /gene="alaS"
                     /locus_tag="VF089_14170"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6175156.1"
                     /translation="MITGNNIRTSFLDYFTRQSHTVVKSSSLVPDKDPTLLFTNAGMV
                     QFKNVFLGQERLPYSRAASSQKCLRISGKHNDLEAVGRDTYHHTFFEMLGNWSFGDYY
                     KAEAIEWAWKLLTREWSLPKDKLWATVYLDDDESERLWARISGLPAERIRRFGEKENF
                     WEMGETGPCGPCSEIHLDRGPEACDRKGLPAHACQVNGDCARYIELWNLVFIQYNRKD
                     NRELEELPSKHVDTGMGLERITAVLQDVLSNYDIDYMRELTAAVENLTGKRYGADPTA
                     DISFRVIDDHARAVSFLIADGVMPSNEGRGYVLRRLLRRAARHGRLIGLKEPFLYQIA
                     STVGEVMGEAYPELRAEAQRIREIIRGEEERFSETLEKGLVILEDAAAKLKQQNHKML
                     PGDVAFRLYDTYGFPLDLTEDILRTENISVDQQGFENLMETQRSRGREARAVAMEVFD
                     QVAVTDAVTATKFVGYDHLEAESTVVALYANGAGKTEAVEGDVIDLLTAVTPFYGESG
                     GQVGDRGTITSARGDLIQVMDTQHPTPQLTVHRGKVKKGRIQVGDKVHLEVDQEKRQK
                     TRLNHSATHILHAVMREQLGSHVRQAGSLVTPERLRFDFNHTGAISDE"
BASE COUNT         1689 a         1803 c         1997 g         1526 t
ORIGIN      
        1 gtgagaaggc tgtttttggg cgagtcctaa gacgtcctgc caccggtttg tggattgaca
       61 cacccagcta aacggtttat cagtgtaaag tttgtatgca acttggattc ccatatgggg
      121 gccatcaacc gcaccccccc caggcgctgc agttcaaaag tcttgggtct gtatatagcc
      181 tgctgatctg gttgtgcgta cttatcgcga ctggagccac gaaatccgca gaagccgacg
      241 ctagcagaca aacggggggg cgcaaaggga tcgccaaggg caaagtacca gcccctaggc
      301 cgccggttca ccgccttacg aatcaaacgt tgctgcctcc ccgacaggca acggtctcgg
      361 atctctggca taggcaagag tacatcgttc atcatgtccg ttctggagat accttaccga
      421 aaattctaga ccgatttcgc ctgacgccgg gtgacaagga gctctggggc cgctcttttc
      481 gccaacattt ccggtcagga aagctggctc ctggaagaca agttcatttc tacttcgcca
      541 tgccggatgt cgaccggaac cgcggtggcg aatcgttgat ggcggtggag gtcgacttca
      601 acgactcaat ggatttgacc tgggaaaaga ccggccggaa tttctttttc gcacgtcggg
      661 aaaagccggt caatgtcgag ctgagaaccg ctaccgcaac catcgatggc tccctgttca
      721 cctccgggtt aaaggcgggt attcacagta gcctactttc acaattagcc gatatcttca
      781 cctgggatgt cgatcttgaa aaagacatct atcaaggcga cagcttcaag atcttatacg
      841 aacagcgcag cacaaggggt caaccgaaca agccgcagct gcgaattctg gccgcagagt
      901 tggtcaatgc cggcgagaag aagaccgcga tttacttcga gaagcagaaa ggcatgggcg
      961 cgtattataa catcgaaggc agaagcttag cccgctcatt tcttcgcttc ccattggagt
     1021 ttgccaatat aacctcgagg ttcagcgagt cgcgctttca tcccgtctta aaaacgagtc
     1081 tgccgcacac cggcgtcgat tttgccgccg aacggggtac gcccgtccga gctgtcggcg
     1141 acggtattat taatcaggtt ggttggagcg gcagttatgg caagttagtc gaaattcagc
     1201 atgatgccag ctttacaacc cgctacgcac acctttccgg ctatgggaat ggtatccgga
     1261 gaggtacctt cgtaaaaaag ggacaggtaa ttggatttgt cggcgccacc ggcagaacca
     1321 ccggtcccca tcttcatttc gagctctaca aagatcaaca atacataaac ccgctcagtc
     1381 tcgatttccc tgccgaagac accatcgaac ccgcgctgtt aacgctgttt gaagatcaaa
     1441 aaaatacctt ccttgttgaa ttgacgtcct tgcctcagtc ctagttcaat tctgttccat
     1501 gggtcagccc tttcactatc ttaaacacgt tggtttctgc tgaacatcgc agtaccatcg
     1561 gctgaaactg aacacaaaaa gacgtttgcc ttcacggaac cacagacttc cttccgctat
     1621 cccaagccgc tcggcgcctg atctctcttc aattctcgga acactgcagg caacggcggc
     1681 tgtggacgct gtgatggtcg ggttgtacac tctggtcatt ctgcagccat aagtataaat
     1741 ggttgggcat tgatatgatt cagaaggtcg taatcttgag cactggcgac gaactcacca
     1801 gcggacgagt gatcgacacg aactcggcag agatcgcaga gcgccttttc gcgataggaa
     1861 ttgaggtgcc ggcgattctg aaggttggtg acaataaaga tcagctccta tgggcgcttc
     1921 gccaggcgca ggctctggga gatagtgtga tcggcaccgg cgggcttgga ccgaccagcg
     1981 atgatctcac aagcgaagtc gtcgcggagt ttctcgggcg taagcttcga cagaacgagc
     2041 acgtggcgag ggaacttcag cagcgcttta aagagcgagg tatcacttgg tcggccaata
     2101 ccttgaagca ggcgctgttt ccagagggat cgacgatcat ttctaatccg gtggggacag
     2161 ctcccggatt tcacgtgccg attccgccgg ataagacact cctctggctt tccggggtgc
     2221 cacaagagat ggttgctatg cttgatcaaa cggttttacc gtggatcgag cgccagcggg
     2281 gcgaagccgc acacgcttct tcctgcacgt tcaagattca cggagtcact gaaggcaagc
     2341 tcgatgaaat gctacgacct gtgcagctgg gaaataatgc gaggttatcg ttccgcgcgc
     2401 attatcccga tctttctttg cgactgaccg ttagaactca gcgggcggat ggatcgcttc
     2461 tttttgaaac gatccagaaa caaatcaggg cactattggg gaagcacatc tatgccgagg
     2521 gaaatacgag tttggaagaa gttatcggcg ggctactcat ggagaagcag cagactctcg
     2581 ccctggctga gtcttgtacc ggcggttaca tcagccatcg ggtgacccgg atcgccggta
     2641 gctcagctta ttactatggc ggtgcggtaa cttattccaa tgaagcgaag atacagtttc
     2701 tcggcgttcg cccagaaact ttgcaagaac acggcgcggt gagtcgcgaa accgccttag
     2761 aaatgtcggc aggtattaag caacgcaccg gtgcagatgt tgggctaagt gtcacgggtg
     2821 tcgcaggtcc tacgggggga agcgcggcca agccggttgg cacggtttgg gtcagtatcg
     2881 ctcaaaacgt aaggcatgaa gcgaaacttt ttaggttctc tggggatcgg gaacacgtta
     2941 ttcttggcac gtcccaggtc gctctgaatt ggcttcgtac attccttctg cgatgaggta
     3001 gcgtgattcg cgcgttcatc ggagtgcaga tcgctcctca ggttcgcgcc cgaatctccg
     3061 aggcaatagc gcacctggcg ccggaacttt ccgggattcg ttgggtcaga gaagagaact
     3121 ttcatttcac gctgaagttt ctaggtgtta ttgaagagcc gcaaatcgag atcattagcg
     3181 acgcgcttgc acgagcgatc cagccctttc ggcggtttac tattagtgct aaaagttttg
     3241 gagcattccc ggaatttaaa ggggctcgcg ttttatgggt cggcctcgaa ggaaatgcta
     3301 tgccggctct ggcaaagact gtcgaatctg tgctagaacc cttcggcttt cccgctgaga
     3361 acagaatttt tcggccccac ttaaccatcg gccgatggcg ccacccaatg ggagtgcagc
     3421 aacaactcga gggaaagctc aagcgctgga agaatttcga gttcggcgag ttcagtgtcg
     3481 acgaggtggt tttatttcga agcgtgctaa gaccggacgg cgcggtgtat cggcccctta
     3541 aagcggtccc actcgccggc gaatgaacga cgtcgttacg gttaatacta aggagacaaa
     3601 gtccatggat gtaaaccggg aaaaagccct cgacctagcg gtaagccaga tcgaaaagca
     3661 attcggcaaa ggcgcaatca tgaagctcgg cgagggaggt gtgacaaaag acgttcccgt
     3721 gatctcgacc ggctctctcg ggctcgacat cgccctcggt attggcggcg tgccgcgcgg
     3781 ccgagtaatc gagatatacg gccctgaatc gtcgggtaag acgaccttgg cattgcaaat
     3841 cgtcgccgaa gcgcagaagc aaggcggtat gggcgcatac gtagatgcgg agcatgcgtt
     3901 agatctcggt tacgcgaaga agcttggagt caagacggac gatttgctag tctcacaacc
     3961 ggaccatggc gaacaagcgt tggaaatagc cgagactctg gtccgtagcg gcgccatcga
     4021 tgtcctagtg atcgactcgg tcgccgcgct cgtgccgaaa gcggagattg aaggtgagat
     4081 gggtgattcg cacatgggat tgcaagcgcg gcttatgtcc caggccctac gcaagctcac
     4141 cggaaccatc gcgcgctccc acaccgtcgt tatctttatt aaccagattc gcatgaagat
     4201 cggcgtgatg ttcggcaatc ccgaaaccac taccggtggc aacgctctaa agttttatgc
     4261 gtccgtgcgg atggatattc gccgcatcgg cgcattgaag gacggtgata atattgtcgg
     4321 tgggcgcact cgcgtgaagg tggtcaaaaa caagatggct ccaccattca aagaagccga
     4381 gttcgacgtg ctttatggaa ctggtatttc ccgcgatggg gagatcgtcg atctgggcag
     4441 tgaaatgggc atcgtcgaga aaagcggcgc ctggtattct ttcggcggcg atagaatcgg
     4501 ccagggacgc gaagcggcga agaactttct caaagaacat cccgaaatcg cgcagcaaat
     4561 catgtcaaaa gtgatggagc gtgtcggctt gaagcgagag gaagcccctg tcacggccat
     4621 taacaccgac aggaaagaaa aagcaaaagt aaaataaaga aagtctcgga actgaaccga
     4681 acgattcaca aacccaaatc gcctgactcg gaggaggagg cgcttcaatc cgcctttcga
     4741 tatctcagtt accgcgcccg cagtgaagcg gaactactca cccatctatg ccgcaaaggc
     4801 tattcagccg ctgcctgcca gaaggctctg gagaaactcc gcactctcaa ttacattaat
     4861 gatgagtcct ttgctcggag ttgggtttgg tccagagtcg ccggccgcaa ctacggaccc
     4921 aggagaattg aacaggaatt aagggccaaa ggcgtcgaac aaccgttgat ccgagacgta
     4981 atcggcgcga tcttcgcttg cggcgacgag gaaaaaaacg ccaaaagctt attggagaag
     5041 agatttaagg gggtcgatct tggagatccc aaagtccttc gccgggcgag cgcgtttctc
     5101 gcacgccgtg gttacagcag tgaggtgatt tttagtttga tgcggcgccg cccggacgac
     5161 gatttagagc catgatcact ggaaacaaca ttcgcacatc ctttttggat tacttcacca
     5221 ggcaatctca tacggttgtg aaaagttctt cgctggtgcc ggacaaagac ccgactttgc
     5281 tcttcaccaa cgccggcatg gtgcaattta agaacgtttt ccttggccag gaacgactgc
     5341 cctactcgcg cgccgcaagc tcgcagaagt gcctgcgcat cagcggcaaa cacaacgact
     5401 tggaggccgt cggcagggat acgtaccacc acaccttctt tgagatgttg ggcaactggt
     5461 ccttcggcga ttattataag gccgaagcga ttgaatgggc gtggaagctg ctgacccgag
     5521 agtggagttt gcccaaagac aagctctggg cgacggtata tctcgacgac gatgaatctg
     5581 agcgtctttg ggccagaatc agcgggctgc cggctgagcg cattcgccgc tttggggaga
     5641 aagaaaactt ttgggagatg ggcgagaccg ggccgtgcgg gccctgtagc gagatccatc
     5701 tcgaccgcgg acctgaagcg tgtgatagga agggtctgcc tgcccacgcc tgccaagtca
     5761 atggcgattg cgcgcggtac atcgagctct ggaatcttgt ttttattcag tacaaccgca
     5821 aagacaaccg cgagctggaa gagctgccgt caaaacatgt cgataccggt atgggcttgg
     5881 aacgcattac ggcggtgctg caagacgtgc tttcgaatta cgatatcgac tacatgcgcg
     5941 agctgaccgc ggcagttgaa aacctgactg gaaaacgtta cggcgccgat cccacggccg
     6001 atatttcgtt tcgtgtgatt gacgaccacg ccagagctgt cagcttcctc attgccgatg
     6061 gtgtgatgcc cagcaatgaa gggcgtggct acgtcctacg acggttgctt agaagggcgg
     6121 cgcgtcacgg tcgactgatt ggattgaagg agccgtttct ttaccaaatc gcgagcaccg
     6181 tcggagaagt aatgggcgag gcctatcctg agctccgagc tgaagcacag cggatccgtg
     6241 agatcatccg cggtgaggaa gagcgcttca gcgaaacctt ggaaaaggga ctggtgatcc
     6301 tggaagacgc ggccgcaaaa ctcaagcagc aaaaccacaa gatgttgcca ggcgacgttg
     6361 cgtttcgcct gtatgacact tacggttttc cgttggacct gaccgaagac attcttcgca
     6421 ccgagaatat ctccgtcgat cagcagggtt tcgaaaatct catggagaca cagcgttccc
     6481 gcggtcgtga agcccgcgcc gttgcgatgg aggtttttga ccaggttgcg gttacggatg
     6541 cggtgacagc gactaagttc gtcggctacg atcatcttga ggcagagtcg accgtcgtcg
     6601 cgctatatgc caacggagcc ggtaaaacag aagcggtgga aggcgatgtg attgatctac
     6661 tcacggctgt cacgcctttt tacggagagt cgggcggtca ggttggtgat cggggtacca
     6721 ttacaagtgc gcgaggcgat ctcatccaag taatggatac ccaacatccg acgccgcaac
     6781 tgacagttca tcgcggtaaa gtaaagaaag gacggattca ggtcggtgac aaagtgcatc
     6841 tggaagtcga tcaagaaaag cgccagaaga ccagattgaa ccactcggct acgcatatcc
     6901 ttcacgcggt gatgcgagag caactaggca gccatgtgcg gcaggccggt tctctggtta
     6961 cgccggaacg gctgcgtttc gattttaatc acaccggtgc aatatccgat gagaa
//
LOCUS       DASUTG010000613        10006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_985740, whole genome shotgun sequence.
ACCESSION   DASUTG010000613 DASUTG010000000
VERSION     DASUTG010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985740"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VF089_14175"
     CDS             complement(<1..888)
                     /locus_tag="VF089_14175"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175157.1"
                     /translation="MKKILTVVLALCCLALPLHSAAQEKFRAASGGFSTAIHALLWLA
                     YEKKIFQKYGLDGEYLALESGTPAMQALLANELQIVFTTGALAITANLQGADATIIAG
                     GINFIPNKLLVRPEIKTPEGLKGKRIAISRFGSASDYATQVALEKLGINPKDVTIVQT
                     GGNMTRLAALSQGTVHATLLSEPHTTVGIREFKLNSLIDLAESGVAFPQNCFIVKRSY
                     FEANRPKIVNAMNAIIESYFILRKDKPLAVSLMKKYNRVSDEDASLGYDYYLAKYGDG
                     VLVLPDRKGLEFIISQVAAT"
     gene            1072..2076
                     /locus_tag="VF089_14180"
     CDS             1072..2076
                     /locus_tag="VF089_14180"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175158.1"
                     /translation="MKRHWIIAFLILAIAVCSLSRSAAAQQLEKLAIGHSNLRNDIAA
                     LWVPKDIGIFRKNGLDANIVLITGGQRMTQALLSGSAPLGFTGATPVVTAVAGGADTA
                     MILGINNKLTYDIWAKAEVKKPEDLKGKTLAISSFGSSSHVASFLMLQHFKLDEKRDR
                     ITFLAIGDEPTRAQALIAGGIDATIIDPSVSLQIKDRGFTYLGNLEPLGVPFVNNALV
                     STRTFLKEKPRTAEAVVRSIVEGNAFILNPANRAKVTDILAHHLRLSGDEASRTYEDL
                     LPKVEARPYPNMDAVRATIEIMGSRNPKIARLKPQDVVDTSILERLEQSGSTRSESAK
                     "
     gene            2088..3185
                     /locus_tag="VF089_14185"
     CDS             2088..3185
                     /locus_tag="VF089_14185"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6175159.1"
                     /translation="MIFLTNAHVEQVLDMPTCIQAMEEAYHELNQLRAGYRPRIDFYV
                     PQEPHYYRWGTMEGASRKLGVFAIRMKSDMLAWENKDGFIVEDKYCIERGTYCGLIFL
                     TSTRNGEPLAIMNDGYLQHMRVGACAGLGTKYLARQNSKVLAMIGSGGMARTYAEAIK
                     HVRPIEVMRVFSPTKTNRQAYAAEMTGKLDIEVIAVDSPEKALKGADVVALTTDSLVP
                     VIKAEWLEPGMHINNVRNNEAGPDVLKRADVIARLGTSTLFADRNAPEVTTGSDGMFG
                     YIAGNAEEKKKIPSAPHYEIDNPEIGTVPDIMAGRWVGRANDHQITFLNNQGTQGLQF
                     AAVGGTAYNLARAKGLGHPLPLEWFTQNIRD"
     gene            3292..4086
                     /locus_tag="VF089_14190"
     CDS             3292..4086
                     /locus_tag="VF089_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175160.1"
                     /translation="MATLRYIAYLSGDPEKLSDFYRRFLGAEELGRSAEGDISITDGF
                     YNLTFFKRRAQLNEMKMECGLHHIGLAVGDLEETKGRFLALNPRGMILQEPDDIHHGE
                     IRIHDPECFPVSLSTRQFGVPAEKEKKFPRVRHIAYNALDPEMMLRFYTQVFGLREVP
                     SSYQRRQQGRGNRFCGDGQTNLAIHPFYNPIEGHEARFGINHIGFLTDKMQATLAELR
                     KVLDIQPRPSTRPYAEVRFRDPEGNALDLSQTKGWEVDVNKWETAV"
     gene            4512..5525
                     /locus_tag="VF089_14195"
     CDS             4512..5525
                     /locus_tag="VF089_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175161.1"
                     /translation="MKGTRTIAASLTMLISLLSQFKSSTAASAPLENLTIGWSAVAGS
                     QAPFWITKEAGLFEKNGLDVVMIYLDGGSRATQAMMSGDVPIAQVGGNAPIVARLRGA
                     DVTLISGLLNVLAYSMVVAPEIKRPEDLRGKKLTVSRFGSNSDYATRKILLKWGLKPD
                     VDVAILQIPGGQPTRLAAIQSKQVHGMVAQPPVTNMARKFNMNIIAQPEDFGGAYPTT
                     PVASRVSYIREKRDTARKFTRALLQGIHLYKTDKEFSKKVIAKYVKTNDNDALEDSYQ
                     FFSRLVPEKPYPSLESVKEALAELGEKDAKARAARPEDFVDMSLIKEFDDNGFIDGLY
                     KKR"
     gene            5884..9153
                     /locus_tag="VF089_14200"
     CDS             5884..9153
                     /locus_tag="VF089_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6175162.1"
                     /translation="MGDHRSAKLNGEQLSVDEVLNTSELFRRAPRAPEYAAENRALIA
                     LAQAMNEAPRTFLQKLADIALELCHADSAGISILETSEGASVFRWRAVAGRYAPNINT
                     SVARDACPCGTVLQRGSGLLFDRPERHFVALREMNLPVLENLTVPFHSAGKPVGTLWV
                     LSHTPERKFDAEDARLLTSLSRFASAAYDMIGTVEAAASKHTEKEFSESEQEFRAMFE
                     LSSVGQCQVDPLTGQFVRVNRKMCEMTGYSAEELTLRSISELTHPDDRDTTASLLRQL
                     SRGEIPVISIETKLLRKDGSTFWNHLNVSLLQRPAGQAPRASAIFRNISARKETEQRL
                     YEAEERMRLAMQAAGVFAWDVDLLTGTIKYSENMAQLFGYETLPEKYSSVAGATAVIH
                     PDDREGVIQASLPAFEQGGAFSAECRAEAAVGGYVWIHCNGTVVRDAYGNPARFVGIV
                     QNIEDRKRAEQALQASQERLSRAIQIETVGVIFFRTDGRVTDANDAFLRMSGYSRSDL
                     QLGLVRWDRMTPPEWMPQSLRAVEEFKMMGRTIPYEKEYIRKDGSRWWALFAATRIAA
                     DEGVEFIIDITKSKLAEEKLRESEERFRTIADSSPVMIWVTDDAGRIEFVNRSYLDFF
                     GTTRERAAQLEWSEIVHSDDREYLKAFSAALRERQPLHARARVKRHDNQWRWIESRGN
                     PRLGLAGEVTGYVGSSADITELIKSQEAMREADRRKDEFLATLAHELRNPLAPIRNGL
                     HMLRLTSYSNPATDQTYEMMERQVNQMVRLVDDLLEISRITRGQIELRKERVELAAVI
                     QSAVEVSKPLIEDFEHQLAISLPAEPLNVVADPVRLEQIISNLLNNAAKYTDPGGQIW
                     LTAQRQSDRATISVRDSGIGIPPDMLPRIFEMFTQVEHSTGRTQGGLGIGLTLVRSLV
                     EMHGGNIEARSTGPGRGSEFIIQLPLLDVRSASEIAPADTWCPAQLSPRRVLVVDDNH
                     DAADSLARLLHVVGGDARAVYSGAQALEVLETYKPAVLLVDIGMPEMDGYEFARRIRQ
                     NPDYQNLTLVALTGWGQEEDRRRSKAAGFDFHLTKPADFNALQSLLGSLETPEQSLTR
                     H"
     gene            complement(9244..9567)
                     /locus_tag="VF089_14205"
     CDS             complement(9244..9567)
                     /locus_tag="VF089_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175163.1"
                     /translation="MQNGASAEKVEAVLGDYKENPLFSLRERLALELAERMTYTEQRV
                     TDEFFDRLKEHFTDEELVELAAVRALENCRSKFNPVFGVEANTFCPLPQVKEAAAAAT
                     RRFHE"
     gene            complement(9592..9834)
                     /locus_tag="VF089_14210"
     CDS             complement(9592..9834)
                     /locus_tag="VF089_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175164.1"
                     /translation="MARIKGVTESEANAEVRQVFEAQKKRQGVVSNTAKVYALRPSIQ
                     RGVQALAQGIQESGLISPELKHLLCVKTANINGCPF"
BASE COUNT         2340 a         2781 c         2719 g         2166 t
ORIGIN      
        1 ggtggcggcc acctgcgaaa taataaattc cagccctttg cggtcgggca agaccaacac
       61 gccatcaccg tatttggcca ggtagtagtc atagccgaga ctggcatcct catcgctgac
      121 ccggttgtat ttcttcatca acgagacggc tagcggtttg tccttgcgca aaatgaaata
      181 actctcgatg atcgcattca tggcattgac gatcttcgga cgattggcct cgaagtagct
      241 gcgctttacg atgaagcaat tctgcgggaa ggccacgccg ctctcggcca gatcgattag
      301 cgaatttagc ttgaattctc ttataccgac agtggtgtgc ggttcgctta acaaggtcgc
      361 gtgcacggtg ccctgactca aagccgcaag ccgcgtcata ttgccgccgg tctggacgat
      421 ggtcacgtcc ttggggttga tgccgagctt ttccagcgcc acttgagtag cgtaatccga
      481 tgccgaaccg aagcggctga tggcgattcg tttccctttc aaaccttccg gcgttttaat
      541 ctcgggacga accagcagct tattgggaat gaagttgatg ccaccggcga taattgtcgc
      601 atcggctccc tgcagattgg cggtgatcgc tagagcgccg gtggtaaaga caatttgcag
      661 ttcgttcgcc aacagcgcct gcatcgccgg cgtgccgctc tccagcgcca ggtattcgcc
      721 gtccaggccg tatttctgaa aaatcttttt ctcgtaagcg agccacaaga gcgcatggat
      781 ggcagtcgaa aaaccgccgc tggcggcgcg gaatttctcc tgggcggcag agtgcagcgg
      841 taatgccaag caacacaatg cgagaacgac ggttaaaatc tttttcataa agttccctta
      901 ttagtctcca cccaatcagg tttgaacacg gaccaacttc actgtcaaga taacttcgct
      961 cctgcgaccg ccgctgacat ggaactgttg cagtaaattc ggattgttga cttttcgcag
     1021 gcggctcacg taagtaggta gtagatcgct ccgcatgtaa ggatttcgta gatgaagcgc
     1081 cattggataa tcgctttctt aattttggcc atcgccgttt gttcgttgtc acgatcggcg
     1141 gcggcgcaac aacttgaaaa gctcgccatc ggccattcta acttgcgcaa cgatatcgcc
     1201 gcgctctggg tgcccaaaga catcggcatc ttccgcaaga acggcttgga tgccaacatc
     1261 gtcctgatta ccggcggtca acgcatgact caagcgctcc tctcgggttc tgccccgctg
     1321 gggtttaccg gagccacacc ggtggtcaca gcggttgccg gcggcgccga caccgcgatg
     1381 atcctcggca tcaataacaa actcacttac gacatttggg caaaggccga agttaaaaag
     1441 ccggaggatt taaaaggcaa gactctggcg atcagcagct ttggcagctc atcacatgtc
     1501 gcatcgtttc tcatgctgca acacttcaag cttgatgaga aacgcgaccg catcacattt
     1561 ttggccatcg gcgacgaacc cactcgggcg caagcgctga tcgctggagg aatcgacgcg
     1621 acgattatag atccctcggt ctcgctgcag atcaaagacc gcgggttcac ctatctcggc
     1681 aacctcgagc ctttgggagt tccattcgtc aacaacgcgc tggtttcgac tcgcacattc
     1741 ctcaaagaaa agccccgcac ggcggaggct gtcgtccgat cgatcgtcga aggcaacgcc
     1801 ttcatcctca acccggccaa ccgggccaaa gtcacggaca tcctggccca tcatctacga
     1861 ctaagcggcg acgaagccag ccgaacatat gaagacttac tgcccaaagt cgaagcgcgg
     1921 ccttatccga atatggacgc ggtcagagcg accattgaga tcatgggttc gcgaaatccc
     1981 aaaatagccc ggttgaaacc gcaagatgtc gtcgatactt cgattcttga gcgcctggag
     2041 caaagcggat cgacccgctc ggagtcagca aaataacagg agagatcatg atttttctta
     2101 ccaatgctca tgttgagcag gtcctggaca tgcccacctg cattcaagcg atggaggaag
     2161 cgtaccacga gcttaatcaa ctgcgagccg ggtaccggcc gcgaatcgat ttttatgttc
     2221 cgcaagaacc gcactactat cgttggggaa ccatggaagg cgcatcgcgc aagcttggcg
     2281 tattcgccat ccgcatgaaa tcggacatgc tcgcgtggga aaacaaagac ggcttcatcg
     2341 tcgaggacaa atactgcatc gagcgcggaa cctattgcgg tttgatattt ctcaccagca
     2401 cacgtaacgg cgagccgcta gcgattatga acgacggtta cctgcagcat atgcgggtcg
     2461 gcgcctgcgc cgggctggga acgaagtacc tggcgcggca gaattccaaa gtcctggcga
     2521 tgatcggctc cggcggcatg gcgcgcacct atgccgaagc gatcaaacac gttcgaccca
     2581 tcgaagtcat gcgcgtgttc agcccgacca aaaccaatcg gcaagcttat gccgctgaga
     2641 tgacgggaaa gctggacatc gaagtgatcg ccgtggactc accggaaaaa gcccttaaag
     2701 gagccgacgt ggttgcgctg accaccgatt cgctggttcc ggtgatcaag gccgagtggc
     2761 tcgagcccgg aatgcatatc aacaatgtgc gcaataacga agccggtcct gacgttctaa
     2821 agcgggccga cgtgattgcc cgtctcggca catccacgct gttcgccgac cgcaacgctc
     2881 cggaagtaac caccggcagc gatggtatgt tcggttacat cgccggcaac gccgaggaga
     2941 aaaagaaaat tccgtcggcg ccgcattacg aaatcgacaa cccggagatc ggcaccgttc
     3001 ccgatatcat ggccggacgt tgggtcggcc gcgccaatga tcatcagatc acgtttctaa
     3061 acaaccaggg cacgcaaggc ctgcagttcg ccgccgtcgg cggcacggct tacaacctgg
     3121 cacgcgccaa aggcctggga catccgctgc cgctggaatg gtttacgcaa aacataaggg
     3181 actagaacgg tgggagtgat ggagtcctgg agtgtttggg tagcgaattt cgcagtactc
     3241 caatactcca ttactccaat gacttgaagt ttaatttcca gagagacgcc catggcaacc
     3301 ttacgctata ttgcttacct cagcggtgat ccggaaaagc taagcgactt ctatcgtcgc
     3361 tttctcggcg ccgaagagct cggccgttca gccgaaggcg acatctcgat caccgatgga
     3421 ttttacaacc tgacgttttt caagagacgc gctcaattga acgaaatgaa aatggagtgc
     3481 gggctgcatc atatcggttt ggcagtcggc gatctggaag aaaccaaagg acgatttcta
     3541 gctctgaatc cgcgcggcat gatcctccag gagcctgacg atattcatca cggtgagatc
     3601 aggatccacg atcccgaatg ttttccggta agcttgtcga ctcggcaatt cggtgtgcct
     3661 gctgaaaaag aaaagaagtt tccccgcgtc cgccacatcg cctacaacgc tttggatccg
     3721 gaaatgatgc tgcgctttta cactcaagtg ttcggcctgc gcgaggtccc ctccagctac
     3781 cagcgccggc agcaaggccg tggcaaccgt ttttgtggcg acggtcagac caatttagcg
     3841 atacacccgt tctataaccc catcgagggc catgaggcga gattcggcat caaccacatc
     3901 gggtttctca ccgataagat gcaggcgacc ttagccgaat tgcgcaaggt gctagatatc
     3961 cagcctcgcc cgtcgacacg accttacgcc gaagtccgat ttcgcgatcc ggaaggtaac
     4021 gcgctggatt tgtcgcagac caaagggtgg gaggtcgacg tgaataaatg ggaaacagcg
     4081 gtatagtctt tcaatccaac gttcaaagtt caactgttca aggttcaacg ccagaattga
     4141 atggtaacag tagcattgta gtacgaaagc taatttgttt agcccggatc ccgcagtaga
     4201 gatggatacc tgtagagtga tcgaggggcg agcgtggagg agcgaaggca cagaagcgaa
     4261 caatttcaaa gccgtctatg gttggccttg ggctggcagt tttttcaagc gcccgctaaa
     4321 gcactggagc ttgcagggcc actatcagcc acagacctat tgccttcgtt ccggagggtt
     4381 cgtcccttga tcgctgtgtc acccggaact gcgggattcg gtttagttca actccatcgg
     4441 acagcctgca tcacgcgatt tcacgttgac tgcatgcgcg cactctcatc ataaagaata
     4501 taggagataa catgaaaggc acacgaacca ttgcggccag tttgacgatg ctgatttcgt
     4561 tgctgtcgca atttaaaagt tctaccgcgg cgtccgcgcc tctggaaaac cttacaatcg
     4621 gctggagcgc ggtcgccggc tcgcaagcgc cattttggat caccaaagag gccgggctgt
     4681 tcgaaaagaa cggcctcgac gtagtcatga tctatctcga tggtggttca agagcgaccc
     4741 aggcaatgat gtccggtgat gtgcccatcg ctcaagtcgg cggcaacgcg ccgattgtcg
     4801 ccaggctccg cggagctgac gtcacgctga tcagcggcct gctgaatgtt ctcgcctaca
     4861 gcatggtggt cgcgccggaa atcaagcggc cggaagatct aagaggcaag aagctcacgg
     4921 tcagccgttt tggttccaat tctgactatg caaccaggaa gatactgctc aaatggggct
     4981 tgaagcccga cgtcgacgta gcgattttgc aaatcccggg cggccagcca acccggctcg
     5041 cggcaatcca atcgaaacag gtgcacggca tggtagccca accgccggtg accaatatgg
     5101 ctcgtaaatt caatatgaac atcatcgccc aacctgagga tttcggcggc gcctatccga
     5161 ccacacccgt cgcatcgcgg gtctcctaca ttcgtgagaa gcgtgacacc gcgcgtaaat
     5221 tcacccgagc tctcctgcaa ggcatccatc tctataagac cgacaaggaa ttcagcaaaa
     5281 aagtgatcgc aaaatatgtc aagaccaacg ataacgacgc tttggaggac agctaccaat
     5341 tcttctctcg cctggtgccg gagaaaccgt atccttcgct cgaatccgtc aaagaagcgc
     5401 tagccgagct aggcgagaaa gacgccaaag ctcgcgccgc gcggccggaa gattttgtcg
     5461 atatgagtct gatcaaggag ttcgatgaca acggtttcat cgacgggctg tataagaaac
     5521 gctaatttgc agattcggga gaagccgttt agtgcggttc gatctgaacg aatctcacaa
     5581 gcgccaagat ctgaactcga tgccttccat ccggtgcagt tacaagcttt acaacccgtc
     5641 cgacacgtta tagaaaataa gttcctttac tggatatcga ttcagttaat cccgggtgat
     5701 gtcgaggaac atcctgccct gtcattgaac gatcctacag agcagctctt ttaacaccac
     5761 cgataacgtg ggagctcatt ggattttcga gaatgcttct tactgtcagc caaatccttt
     5821 gacgagcttg cacaatcatg taagcatcag ttgtatcggt aaaaatctcg caaggcgata
     5881 ccgatgggcg atcatcgaag tgcaaagtta aacggcgagc agttgtctgt cgatgaggta
     5941 ctgaacacgt ccgagctttt tcggcgtgct ccgcgtgcgc ccgaatacgc ggcggaaaac
     6001 cgagctctga tcgcattggc gcaggcgatg aacgaagccc cgcgcacgtt cttgcaaaaa
     6061 ttggcggaca tagcgctcga gctgtgccac gccgattccg ccgggattag cattctggaa
     6121 acatctgaag gggccagcgt cttccgttgg cgcgccgtag ccggacgtta tgcgccgaac
     6181 atcaacacat cggtggcgcg cgacgcctgc ccgtgcggaa ccgtgctgca acgcggctct
     6241 ggccttctgt tcgatcgccc tgaacggcat ttcgtcgctc tccgcgagat gaacttgccg
     6301 gtccttgaaa atctcacggt gccatttcac agcgctggta aaccggtggg caccttgtgg
     6361 gttctgtctc atactccgga acggaagttc gatgccgagg acgcgcggct gctgactagc
     6421 ctctcccgat tcgcatccgc ggcatacgac atgattggca ctgtcgaagc cgccgcttcg
     6481 aagcacacgg aaaaagagtt ctctgaaagt gaacaagaat tccgtgccat gtttgagctc
     6541 tcgagtgtcg gccaatgtca ggtggatccg ctcactgggc aattcgtccg ggtcaaccgt
     6601 aaaatgtgcg agatgactgg ctactccgcg gaagaattga cgcttcgcag tatcagcgaa
     6661 ctgacgcatc cggatgaccg cgacaccacc gcgtctttac tgcggcaact atcgcgcggc
     6721 gaaatcccgg tgatctctat cgagaccaag ttgctgcgca aagatggctc gactttttgg
     6781 aaccacttga acgtcagcct gctgcagcgc cccgccgggc aagcgccgcg tgccagcgcc
     6841 atttttcgca acatttccgc gcgcaaggaa actgaacaac gattgtacga agccgaggag
     6901 cgcatgcgac tggcgatgca ggctgccggt gtgtttgcgt gggacgtcga cctattgacc
     6961 ggtacgatca aatattcgga gaacatggcg caacttttcg gctatgagac actgccggaa
     7021 aaatactctt cggtggccgg cgcaactgcc gtgattcacc ccgatgaccg cgagggtgtc
     7081 atccaagctt cgctgccggc atttgaacaa ggcggagcct tctcggccga gtgccgcgct
     7141 gaagccgcgg ttggcggcta tgtctggatc cattgtaacg gaacggtggt gcgcgatgct
     7201 tacggcaacc cagcgcgctt cgtcggcatt gtccaaaaca ttgaggatcg caagcgcgcc
     7261 gagcaggcgc tacaggcttc gcaggagcga ctgagtcgcg cgatacagat tgaaacggtg
     7321 ggggtgatat tttttagaac cgatggccgc gttaccgatg ccaacgacgc cttcctgcgc
     7381 atgagcggct atagccgcag cgatctgcaa ctcgggctgg tgcgttggga taggatgact
     7441 ccgcccgaat ggatgccgca atcgctgcga gccgtggagg agttcaagat gatgggccgg
     7501 acgattccct acgaaaaaga atatatccgc aaggatggat cgcgctggtg ggctttgttc
     7561 gcggctacac ggatcgcagc ggatgagggt gttgagttca tcattgacat taccaaaagc
     7621 aaattagcgg aagaaaagct gcgcgaaagc gaagaacgtt ttcgcacgat cgctgacagt
     7681 tccccggtga tgatttgggt aaccgatgac gccggccgca tcgaattcgt caatcgctcc
     7741 tatttggatt tctttgggac tacccgggaa agagccgccc agctcgaatg gtccgagatt
     7801 gttcattccg acgatcgaga gtacttgaag gcattcagcg ccgccctgcg cgagcgacag
     7861 ccactgcatg cgcgcgcgcg ggtcaaacgc cacgataacc aatggcgctg gattgaatcg
     7921 cgcggtaatc cgcgcctggg cctcgcagga gaagtaaccg gctatgtcgg aagttccgcc
     7981 gatatcacag agctcatcaa atcgcaggaa gcgatgcgcg aggccgaccg gcggaaggat
     8041 gaatttctcg cgactttggc gcatgagctg cgtaatccgc tcgcccctat tcgcaacggc
     8101 ctgcatatgc ttcgacttac cagttatagt aatccagcca ctgatcagac gtacgaaatg
     8161 atggaacggc aggtcaatca gatggtacgt ttggtcgatg acctgctgga gatatcccgc
     8221 attactcgcg gtcaaatcga gctacgcaaa gagcgcgttg aactggcggc cgttatccaa
     8281 agcgcggtcg aagttagcaa gccactaatc gaagatttcg agcatcaact cgcgatttcg
     8341 ctgcccgccg agccgctcaa tgttgtagcg gatcccgtac ggctagagca gattatttcc
     8401 aacctgctca ataacgctgc aaagtatacc gatccgggag gacaaatctg gctgacggcg
     8461 cagcgacaga gcgaccgtgc aaccatctcg gtccgggaca gcggcatcgg cattccgcca
     8521 gacatgctgc cgcgtatctt tgaaatgttc acgcaagtcg agcactcgac cggccgaact
     8581 caaggtgggt tgggcatcgg gctcactttg gttcgcagcc tcgtggaaat gcacggaggc
     8641 aatatcgaag ctcgtagcac agggcccggg aggggcagcg aatttatcat tcaactgccg
     8701 ctactagatg tcaggtccgc tagcgaaatt gcaccggccg acacgtggtg ccccgcgcag
     8761 ctatcgccaa ggcgcgtctt agtggttgat gacaaccatg acgcggccga cagtctcgcc
     8821 agattactgc atgttgtagg aggcgacgcc cgggctgtgt acagcggtgc ccaagcgctg
     8881 gaggtactcg aaacctacaa gcccgccgtg ctgctagttg atattgggat gcccgagatg
     8941 gacggctatg agtttgcccg ccgaattcgc caaaaccccg attatcagaa cctgacccta
     9001 gttgcactta ccggatgggg tcaagaggaa gatcgccggc gctcgaaagc agcgggattt
     9061 gattttcacc tgacaaaacc ggctgatttc aacgctctgc agtcgctgct cggctcgctg
     9121 gaaaccccgg agcaaagcct gacgagacac tgaagctgcc ccaatgcggc gcgtacgatc
     9181 tgctcccaga ttacctgtgg aatgaagtta ttgtgcagca acggtaatgc ttacggtgtc
     9241 gtttcattcg tgaaatcggc gggtcgcggc agcggcagcc tctttcactt gtggcagagg
     9301 gcaaaaagta tttgcctcca ctccgaacac cggattgaac ttgctgcgac aattttccag
     9361 agctctgacc gccgctaatt ccaccaattc ttcgtcggtg aagtgttcct tgagccgatc
     9421 gaagaactca tccgtcaccc gttgttccgt gtaggtcatg cgctcggcta actccaacgc
     9481 caaccgctcg cgcaacgaga atagcggatt ctccttgtaa tctcccagca ctgcttcgac
     9541 tttttccgcc gaagccccat tttgcatcgc actggatgcg ttactgtcaa ttcaaaaggg
     9601 gcagccgttg atattggcgg tcttaacaca cagaaggtgc tttaactctg gcgaaatgag
     9661 ccctgattcc tgaatccctt gggccaacgc ctgaactcct ctctgaatgc taggtctcaa
     9721 cgcatacact tttgcagtat tcgaaaccac gccttggcgc ttcttctgcg cttcaaaaac
     9781 ttgtcgcacc tcagcgtttg cttcactctc ggtcacgcct ttgattctcg ccatactccc
     9841 tccgctgtca tattaattgt gattctcaat aagcttacat ggaatccgat gctcttctac
     9901 aacgttgcag atctcccctg acccgtttta gctggactat tgtagcctgg agctaatttc
     9961 tcatctttta ggctggatgc tagctctgtt cggggttaca tttaga
//
LOCUS       DASUTG010000614         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_540297, whole genome shotgun sequence.
ACCESSION   DASUTG010000614 DASUTG010000000
VERSION     DASUTG010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540297"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..2265
                     /gene="ftsH"
                     /locus_tag="VF089_14215"
     CDS             142..2265
                     /gene="ftsH"
                     /locus_tag="VF089_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6175165.1"
                     /translation="MSSIGQKMRRSTDSLPPGRGQPAKTRPGDGKGAEQWKMPPGRTW
                     LWFVGILLANYLLVRFFVPGPEAPITVPYTLFKEQVGKGNVEAIYSQGESITGRFKDP
                     VTYPPAVEKDAASDGKSEGGNEKDAAAPSGAPPKGPERLGPPKTSRNFTTTLPSFVDS
                     GLETFLIENQVEISAKPIDEGGGWATLLFGFGPALLFIAFYVWIFRRAAQQGGGLGGG
                     IMGIGKSKARRYDQEKDTKVTFEDVAGIDEAENELVEIVDFLKDPKKYTRLGGAAPKG
                     VLLIGAPGTGKTLLAKAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQAREHAP
                     AIVFIDELDAIGRARGQVAIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVL
                     DKALLRPGRFDRRVVVNLPDKGGREAILKVHTRNVPLARDASLQEIAAATPGFSGADL
                     KNLVNEAALLAARREQSDVGQKDFLDALEKIVLGPERPLLLSRADKERIAYHEGGHAI
                     LGLVVPGADPVHRVTIVPRGQALGVTYQRPDSDRYNYPEAYLRARIVGMLGGRAAEEI
                     VYGTKTTGAESDIEQATGLARRMVTRWGMSERLGLVQLAPRENPYLNGAGGYAGSRPF
                     SEETAEAIDAEVLKIIGDSHDQAKRLLSAHRKQLDALVEALLARETLNEQEILEVTGL
                     PPAPPLETRMLSVINDGSGRSSPSR"
     gene            2542..5721
                     /locus_tag="VF089_14220"
     CDS             2542..5721
                     /locus_tag="VF089_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX6175166.1"
                     /translation="MTSIAPIVTLSPRDYDAVLFDLDGVLTQTASVHAAAWKRLFDGF
                     LQQRAARTGETFVGFDIDSDYRRYVDGKPRYDGVAAFLHSRRIQLPWGTREDGPGAQT
                     VYGLGNLKDRYFTEQLEQHGVEVYEAAIVLVRTLREHEIKTAVVSSSNNCAAVLESAG
                     IAQLFDTRVDGLDLTHLSLNGKPAPDAFLEAARRLEVEPSRAVIVEDAIAGVQAGRAG
                     QFGCVIGVDHHGQSQALRDAGADVVVTTLAQVKIVTEAPSTWSLVYEGFDPAREGIRE
                     SLCALGNGYFTTRAAVLWSAADGVHYPGTYLAGGYDRMRTNIAGRSVENEDLVNFPNW
                     LALEFRIGDNDWFDARTVKILSYQQELDLRRGMLLRTLSFEDPQGRRTTLKERRLISM
                     ADMHLGALELALTPENWSGKIAIRSAIDGRIVNAGAKLYRKFNNKHLEPLLAEILDDD
                     SVFMLVRTCQSKICVAQAARTQAFIDGEIRAVPRRLIDKPEHIGQEFVLDLQQGQNLV
                     LEKLAFLYTSRDYAISECGLAARKAIGRAGRFESVIAEHVLAWKHLWRRFDVHMQPAA
                     PGFKLNVPMLLRLNMLHLLQAVSPSSIGLDIGVPPRGWTGEAYQGHIFWDELFIFPFL
                     NYRMPEITRSLLLYRYRRLGEARAAAKEAGFHGAMFPWQSGSDGQEETQELNLNPHSL
                     RWLPDNSYRQRHVGSAIAYNVWQYFQVTRDIEFLQFHGAELILEIARFWSSIATFNDA
                     RGRYEIRGVMGPDEFHEGYPGAPAPGLNNNAYTNIMAVWVLCRALDVLDLLPDIRQAE
                     LMTRLGLLPQEIARWRDISRRMFVPFHDNGIISQFEGYENLDELDWQGYRARYGNIQR
                     LELILEAENDSANRYKVSKQADVLMLFYLFSAEELGELFARIGYPFDREIIPRNVAYY
                     DSRSTHGSTLSRVVHAWVLARSDRPRAMRYFAEALQSDVNDIQQGTTVEGVHLGAMAG
                     TVDLVQRVSTGIEVTSDVLRLNPQLPEELERLDLRIRYRGHSLDLRLTRDALTVRCRE
                     PSVAPINLGFKNEVYEFAGGTRVFKLERT"
BASE COUNT         1191 a         1692 c         1786 g         1159 t
ORIGIN      
        1 caggagcgag cagatgaatg tgttaggtgt aactttggtg gacgtggtta atcttgttgt
       61 cattgggtgt gaatgatagt cctcattcat cattgcaggt tttttttgcc atccggcaaa
      121 cggtgttcgt aggagggttc catgagttcg atcggacaga agatgcggcg cagtactgac
      181 tccttgccgc ccggccgcgg ccagccggcg aagactagac ctggagacgg gaagggcgcc
      241 gagcagtgga agatgccgcc cgggagaacc tggctctggt tcgtaggcat cctgctagca
      301 aactacttgc tggtgagatt tttcgtaccg ggccccgaag cgcccatcac agttccctac
      361 acccttttta aggagcaggt gggcaaaggc aatgtcgagg cgatctacag ccagggagag
      421 tccatcaccg gccgcttcaa ggatcctgtg acttacccgc cggcagttga aaaagatgcc
      481 gcctccgatg gcaagtctga aggcggaaac gaaaaagatg cggcggcccc cagcggcgcg
      541 ccgccaaaag gccccgagcg cctcggcccg cccaagactt cgcgtaattt cacgacgacc
      601 ctgcccagct ttgtagattc cggtttggag acattcttga tcgagaacca ggtcgagatc
      661 agcgctaagc ccatcgacga aggcggcggt tgggctacgc tgctcttcgg ttttggcccc
      721 gcccttctgt tcatcgcttt ttacgtctgg atcttccgac gggcggcaca gcaggggggc
      781 ggcttaggcg gcggaatcat gggcatcggc aagagcaagg cgcgccgcta cgatcaggag
      841 aaggatacaa aggtcacgtt cgaggacgtc gccggtatcg atgaagcaga aaacgagctg
      901 gtcgagatcg tcgattttct caaggatccc aagaagtaca cccgcctggg cggggccgct
      961 ccgaaaggcg tgctgttgat cggtgcgcca ggcacaggga agacactgct ggccaaggcg
     1021 gtcgccggcg aggctggcgt gccgtttttc tcgatgagcg cggcggagtt tgtcgagatg
     1081 atcgtcggcg tgggcgcggc acgggtgcga gacctcttca agcaggcgcg cgagcacgca
     1141 cccgccatcg tcttcatcga cgagctcgac gccatcggcc gcgcgcgcgg gcaggtggcg
     1201 attggcggct cgagcgagca ggagcagacg ttaaatcaga ttcttactga aatggatggc
     1261 ttctccagtc gcgagggcat catcgtgctc gccgccacga atcaacctga cgttctcgat
     1321 aaagcgctcc tacggccggg acgcttcgat cggcgggtgg tggttaacct ccccgataag
     1381 ggcggccgcg aggcgattct caaggtgcac acacgcaacg tccccctcgc gagggatgca
     1441 agtttgcagg agattgccgc ggcaaccccg ggtttttcgg gagcggacct gaagaacctc
     1501 gtgaatgagg cggcgctcct ggcggcacgg cgcgagcaga gcgatgtcgg tcagaaggac
     1561 tttctcgacg cgctggaaaa aatcgtgctc ggccccgagc gtccgctgct cttgagccgc
     1621 gccgacaagg aacgcatcgc gtatcacgag ggcggacacg cgatcctcgg gctggtggtg
     1681 cccggcgctg atccggtgca tcgtgtaacg atcgtgcccc gtgggcaggc gcttggtgtg
     1741 acttatcagc gtcccgacag cgatcgctac aactatccgg aagcttatct acgcgcgcga
     1801 atcgtcggca tgctaggagg ccgcgcggct gaggagattg tctacggcac caaaaccacc
     1861 ggagcggaaa gtgacatcga gcaagccacc ggattagcgc gtcggatggt cactcgttgg
     1921 ggcatgagcg agcgcctcgg gttggtgcag cttgcgcccc gagagaaccc gtatctgaac
     1981 ggcgcgggcg gttacgcagg ttcgcggccc ttcagcgaag agaccgcaga agctatcgat
     2041 gccgaggtgc tcaagattat tggcgacagt cacgatcaag ccaagcgcct gttgagcgca
     2101 caccgcaaac aactcgacgc gctggttgaa gctttgttgg cgcgcgagac cctcaacgag
     2161 caggagatcc ttgaggtcac agggctgccg cctgcgccgc ctctggagac caggatgttg
     2221 tcagtaatca atgacggcag cgggaggtca agcccatctc gttgagacac ggctttcacg
     2281 aacagattgc agccggggtt caaccggcta gacagcggct ctccgagaga aaactcaaca
     2341 tctggggatg atcttcaagc atcacagttc taaccgttcg aatgatcaaa agcacattct
     2401 aacgaattct ttcgggttcg attttggatt tggcatttgt tttctttgtt ctgtcaaatc
     2461 cgaaacctcg caaccggatg atctgaacat agaagcgtct atgaagcggg ctcctacttt
     2521 gatgagcacc aagggtaaag gatgaccagc atagctccga ttgtcacttt gtccccgcgg
     2581 gattacgacg cggttttgtt tgacctcgac ggtgtgctga cgcagacggc gagcgtgcac
     2641 gccgcagcat ggaaacggct gttcgacggc tttcttcagc agcgcgctgc aagaacgggc
     2701 gaaaccttcg tcggctttga catcgattcc gactaccgac gctacgtcga tggcaaaccg
     2761 cgttacgacg gagtggccgc atttctccac tcgcgccgaa ttcaactgcc ttggggaaca
     2821 cgggaggatg gcccgggcgc gcaaacggtg tacggcctgg gcaacctgaa ggaccgctat
     2881 ttcaccgaac aactggaaca gcacggtgtc gaagtgtacg aagcggcaat cgtcctggtt
     2941 cgcacgcttc gtgagcacga gatcaagacc gcggtcgtct cgtcgagcaa taattgcgct
     3001 gccgtgctcg agtcagccgg catagcgcag ctcttcgaca cgcgggtgga cgggctcgat
     3061 ctcactcatc tctccctcaa cggcaagccg gcgcccgatg cttttctcga agcagcccgg
     3121 cgcctcgagg tcgaaccgtc tcgcgcggtc atcgtcgaag acgcgatcgc cggcgttcaa
     3181 gcgggacgcg ccggccagtt cggctgcgtc atcggcgtcg atcaccatgg gcaatcccag
     3241 gcgctgcgcg acgccggcgc cgatgttgtc gtgaccacac tagcccaggt gaagatcgta
     3301 accgaggcgc catcgacatg gtcactggta tatgaaggat tcgatcccgc tcgggagggc
     3361 attcgcgaat ctctctgcgc gctgggcaat ggctatttca cgacccgcgc ggctgtgttg
     3421 tggtcggcgg cggatggcgt ccactatccc gggacctatt tggcgggcgg ttacgaccgg
     3481 atgcgcacga atattgccgg tcgcagtgtc gagaacgagg acctggtcaa tttccccaac
     3541 tggctcgcgc tcgagtttcg catcggcgat aacgactggt tcgatgccag aacggtgaag
     3601 atcctgtcct accaacagga gctcgatctc cggcgcggca tgctgctgcg cacgctgagc
     3661 ttcgaagacc cgcagggtcg gcgcacgacc ctcaaggaac gccgcctaat ttcaatggcc
     3721 gacatgcact tgggcgcact ggaactggct ctcaccccgg agaactggtc cggaaaaatc
     3781 gcgattcgtt cggcgataga cgggaggatc gtcaacgccg gcgccaagct ctatcgcaaa
     3841 ttcaacaaca aacacctcga gccgctctta gccgagatct tggacgacga cagtgttttc
     3901 atgctggtgc gcacctgcca gtcaaagatc tgtgtcgcgc aggcggcgcg aacccaagcg
     3961 tttatcgacg gagagattcg cgccgtgccg cgccggctca tcgacaagcc ggaacatatc
     4021 ggccaggaat tcgtactcga cctccagcaa ggccagaacc tggtgctcga gaagctggcg
     4081 tttttgtaca cgtcgcgaga ttacgcgatt tccgagtgtg gcctggcggc gcgcaaggcg
     4141 atcggccgcg ccggtcgctt cgaatcggtg atcgcggagc acgttcttgc ctggaaacac
     4201 ttgtggcgtc gtttcgacgt gcacatgcag ccagccgctc caggcttcaa gctcaatgtg
     4261 ccgatgctgc tgcggttgaa catgcttcac ctgctgcagg ctgtctcgcc cagttccatc
     4321 gggctggata tcggcgtgcc gccgcgcggc tggaccggcg aggcttacca gggtcatatc
     4381 ttctgggacg agctttttat ctttccgttt ctcaattatc gaatgcccga aataacccgt
     4441 tcgctgctgc tgtatcgcta tcggcggctt ggggaagcgc gcgccgcggc aaaagaagcg
     4501 ggctttcacg gagccatgtt tccctggcaa agcggcagcg atggccagga ggaaacccaa
     4561 gaactgaacc tgaacccgca ctcgcttcgc tggctgcctg acaattccta ccggcagcgc
     4621 catgtcggca gcgccatagc ttacaacgtg tggcaatact ttcaggtcac tcgcgatatc
     4681 gagtttttgc agtttcacgg cgccgagctg atcctcgaga tcgcgcgctt ctggtccagt
     4741 atcgccactt tcaatgacgc gcgcgggcgt tatgagattc gcggagtgat ggggccggat
     4801 gagtttcacg agggttatcc gggagcaccg gcccccggcc tcaacaataa cgcctacacc
     4861 aacatcatgg cggtctgggt gctgtgccgc gcgcttgacg tgctcgacct cctccccgat
     4921 attcgtcaag ccgagctcat gacgcggctt ggacttttgc ctcaagagat cgcgcgctgg
     4981 cgcgacatca gccgcaggat gttcgtgccg tttcacgaca atggaatcat cagccagttc
     5041 gagggctatg agaatttgga cgagctggat tggcaggggt atcgcgcccg ctacggcaac
     5101 atccagcgtc tcgagctgat tctcgaagcg gagaacgata gcgccaaccg ttataaggtg
     5161 tcgaaacagg ccgacgtgct gatgttgttt tatctttttt ccgccgagga actgggagag
     5221 ctctttgccc gtatcggtta tccgttcgac cgcgaaatca ttccgcgaaa cgtggcctac
     5281 tacgacagcc gttcgaccca cggctcgaca ttgtcccgtg tagtccatgc ctgggtgttg
     5341 gcgcgctcgg accggccgcg cgcgatgcgc tacttcgctg aggctttaca gagcgatgtg
     5401 aacgacatcc agcagggcac gacggtggag ggagtgcatc tcggcgcgat ggccggaacc
     5461 gtcgatctgg tgcagcgcgt ctccaccgga atcgaagtca ccagcgatgt cctgcggctc
     5521 aatccgcaac tccctgagga gctcgagcgt ctcgacctac gcatccgcta tcgcgggcat
     5581 tcgctcgatc tcagactcac ccgcgacgcg ctcacggtgc gatgccgtga accgagcgtc
     5641 gcgccaatca atctgggatt taagaacgaa gtttacgagt tcgccggcgg cactcgggtg
     5701 ttcaagcttg agagaacatg agcgtcgcga cctttcagcg tgagctagag caaattcgga
     5761 gcatcaaaca atcgcaaaga caaaatagta aatgtttcaa acaaccctga tccggaatgc
     5821 gttctcga
//
LOCUS       DASUTG010000615         7595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_690091, whole genome shotgun sequence.
ACCESSION   DASUTG010000615 DASUTG010000000
VERSION     DASUTG010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690091"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..623
                     /locus_tag="VF089_14225"
     CDS             267..623
                     /locus_tag="VF089_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175167.1"
                     /translation="MKVLVIENHADMREMLVRILDLMGFTAITSRNGKEGVEKALAEK
                     PDLILMDIMMPEISGWEATRILRGNPQTKDIPVLAATVLFRPSDLQRCITVGCNDYIV
                     KPFTVRQLRRKIEQLI"
     gene            1667..1757
                     /locus_tag="VF089_14230"
     tRNA            1667..1757
                     /locus_tag="VF089_14230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1701..1703,aa:Ser,seq:gga)
     gene            1850..1947
                     /gene="ffs"
                     /locus_tag="VF089_14235"
     ncRNA           1850..1947
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF089_14235"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2055..3725
                     /gene="dnaX"
                     /locus_tag="VF089_14240"
     CDS             2055..3725
                     /gene="dnaX"
                     /locus_tag="VF089_14240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX6175168.1"
                     /translation="MSYLVLARKWRPQNFEDVSGQSHITRTLQNAIRAGRIAHAYLFT
                     GVRGVGKTTAARILAKALNCAQGPTPTPCDECAQCKEIIQGNCIDVIEIDGASNRGID
                     EVRQIIENVRYQPARCAFKIYIIDEVHQVTKDAFNALLKTLEEPPASVKFILATTEPH
                     RLPETILSRCQRFDFRRISLKEIVQRLSDISAREGLKISQGALIAVAREADGSMRDAQ
                     SLLEQVLSCVGAEQQQSELDETQLQEILGLAERRMLFDISESVIANDGKRCLELVAQA
                     AEQGRDLTRLSKDLLEHFRNLMVARLCGQKKPEKLADEERAELLDLPDQEIIALSSQA
                     ADVSIDTLLDYFELMAAGEDDVARAANPRFTLETSLVRLAMLPKTLPIIQLIDRLTQL
                     ENRLVSEGKAIPVGMKTLAPRTSTTAGSNSIEATVVPILTSALSQDNEQVWRTFVSFV
                     GKEKKLLASHLETSSSIAIAPESLTIGIAEKHHLSYLQDLENMALLRNFAKRFFSREM
                     TVQLTQLDASNTKSQQEVLASGDTGSEMVKEALRIFGGSIKGVRTDNS"
     gene            3768..4094
                     /locus_tag="VF089_14245"
     CDS             3768..4094
                     /locus_tag="VF089_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX6175169.1"
                     /translation="MAKGIGGMGNLLKQAQEMQSRMAKIQEELAQKTVDGSAGGGMVR
                     VTVNGQLALTAIKIDPTVINPEEKEMLEDLIMAAINDGMRKARDMASSEMSKITGGLK
                     IPGLSV"
     gene            4091..4711
                     /gene="recR"
                     /locus_tag="VF089_14250"
     CDS             4091..4711
                     /gene="recR"
                     /locus_tag="VF089_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX6175170.1"
                     /translation="MSTYPAPLARLVQELSKLPGIGEKTAARLAFHMLKAKKDDVFNL
                     ADSIAKLRKEMGLCRICFGFSEVDPQSGETSVCMICRNPERDRQSICVVEEPSDLIAV
                     ERSQEFRGLYHVLHGTISPLDGIGPDALRIKELLVRIKSEVVQEIIVATNPTMDGEAT
                     ALYLSKVIKPLGVAVTRIARGLPMGGDLEYTDAVTLGKALEGRREI"
     gene            complement(5035..6033)
                     /locus_tag="VF089_14255"
     CDS             complement(5035..6033)
                     /locus_tag="VF089_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX6175171.1"
                     /translation="MLDGNLLLLLVLGLILAAEFVNGWTDSPNAIATVVSTRVLSPYQ
                     ALIMATVLNAIGAMSGTAVAATIGKDIVRPEIINLTTVAAAMVGIIVWSTLAWYYGLP
                     TSESHALIAGLTGAGLATAGPESLVWAGWSKVLIGLLFSSFLGFFGGLLLMAILYRAL
                     AHSRPGKVRRIFGRLQILSAAFMAFSHGSNDGQKFIGVFTLALLLGGILPEFRVPIWV
                     ILLCALTMGVGTAVGGWRIVRTMGLRLTKLEPVHGFAAETAAAVTIEVATRLGIPVST
                     TQTINTAIIGVGATRRLSAVRWGVTFEIVTAWILTFPVCGAIGWLATKLFILFQAV"
     gene            complement(6055..6669)
                     /locus_tag="VF089_14260"
     CDS             complement(6055..6669)
                     /locus_tag="VF089_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEX6175172.1"
                     /translation="MFNFAPHKEKFFDLFKQAAENALAGARALKEMLEHYDNPEESWR
                     KIKDLEHEGDRITHRTIRSLNQTFLTPIDAEDIHALITALDNVMDAIEAAASRMVLFR
                     IDEPTAEARELSDIIVTSTEQLVKAVSHMPRLDDIDEYCIEINRLENTADDIYRKAIG
                     RLFENGTPPLDVIKWLDIYEILESATDRCEDVANTLETVGLKNS"
     gene            complement(7184..>7595)
                     /locus_tag="VF089_14265"
     CDS             complement(7184..>7595)
                     /locus_tag="VF089_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175173.1"
                     /translation="ANKKGIKLESFVDPELPRVSGDFDRLQQLFINLVDNAVKYTPTN
                     GTVTVTVQRTRINGNAGGVEVSVSDTGPGIPEKDIPRLTERFYRVDKARSRDIGGTGL
                     GLAIVKHIAQAHQADLKIESILQKGTTIRVLLRQ"
BASE COUNT         1913 a         1850 c         2037 g         1795 t
ORIGIN      
        1 aaccaacact gtggaacgcg gctaacccga cgcagcttca tcgatgtgtt ttctgcttga
       61 gtcaaaacag taggctgagc aattccgaga aagttagtcg caagatcgta ttctatgatt
      121 tgatctcttc gaacgcaatc gggacatctc ctagagtatt tagggcccgt tctttgtgac
      181 tgactcgaca ggcaggacgc gaatcagctg ctatgatatt cgacagaata cacagacatc
      241 tcacgcttac ctgactaatg ctaatgatga aagttttagt tatcgaaaat cacgcggata
      301 tgcgtgagat gcttgtaaga atacttgatc tcatggggtt taccgcaatc acgtcaagaa
      361 atggtaaaga gggggttgag aaggctctgg ccgagaagcc cgatctgatc ctgatggaca
      421 tcatgatgcc agaaatctct gggtgggagg cgactcgaat actgcgcggc aatccccaaa
      481 ccaaggacat tcccgtactc gcagcaacag tgttattccg gccatctgac cttcagaggt
      541 gcattaccgt gggatgtaac gactacatcg taaagccatt cacggtgcga cagctgcgta
      601 gaaaaattga gcagctaatc taataacctc cagcacgctt ccatgattgt aacgccggac
      661 gcccggttgg tttatagttc atccaatttc gctgctatcg catctgtaat tctcacctga
      721 cgtatgtcgg tgagtgcgaa tctttagata ttggctggat cattagctgc gctagtagga
      781 tatcgtgcga ttgatagctc cggtccacgt cggccaatag aaatcgggaa ttgagtcgga
      841 atggcaatcg cagagaatcc aggaaacgca gagtggtcat aatttggtga aacatggtca
      901 gcgctattct ctccaacgca tgagccctac gatgtccgtt atagagggcg gcaggcgaag
      961 ccgttcggtt ccagccaaaa tggattagga gatgacgcct taagcgatgc gcttaaaaag
     1021 cggcgcctaa tggtcatggc acgcaaattt ctcccgatgg taattgcgcg ctcatcgaat
     1081 tttgagagca tgtggtctaa aggctgccag ccgaaggagc gactctgcct ctaatgttgt
     1141 tcatccaatt gcttccgtct gtatgtcgga acgccgacaa agaaacccga tgagtaggtc
     1201 attttgagtt agcaggcaaa gctgccgtcg cttatccaaa gacagcactc cggcaaccgt
     1261 tccaagactc tcaaagtcat tgaacggcga tgagagtgag ggcataagca ttgctcaatc
     1321 gttccatgcc ggagcgaaga agcgcaactc gctaccacag catggactct tcgttcccgg
     1381 cgccacttca ataagtaatc gcatctgaat tcagccggta gttgaacaat gaattagaaa
     1441 agacctggat gtggcagtct agttgggata tccgacgtaa gcgtcatctc gccgttctca
     1501 atacgaaaag agactgattt atcgcggcgc agatgaaatg ccgagtccgt ggagtgccaa
     1561 tttaaattcg aagattagct ttgtggaaaa caaaacgtcg ctgtggtagt ttccttggtg
     1621 cgcacgcgtt gcgttgtcct aactaactag attaatgggg agagagggag aggtgaccga
     1681 gcggttgaag gtgcacgact ggaaatcgtg tgtactccaa aagagtaccg ggggttcgaa
     1741 tccccccctc tccgccacat gacttaacgc gggcgttgga agcagaagtc tgctataaat
     1801 aggagaccgc tcaaccgata ttttttgaat ttggatgtcg attgctgata gttaggtcct
     1861 gtgcaagaag gagccgcgaa ccccgtcagg tccggaagga agcagcggta ccggaattcc
     1921 tctgtgccat agggtagcct ggctgtcagc aatgggcatc ccagtctctt cgctaattgt
     1981 agcaaaatct tagatcgcgg ccctgttgtt cgatctgaac tttcgaatct gctgagaaat
     2041 ttcgaaggaa ccacatgtct tacctagtcc tcgcgcgtaa gtggcgccca cagaactttg
     2101 aagatgtttc ggggcaaagc cacatcactc gcacattaca gaatgccata cgagcaggcc
     2161 gtatagctca cgcttacttg tttaccgggg ttcgcggggt tggcaaaacc accgcagcta
     2221 ggattctcgc caaagcactg aattgcgctc agggtccgac tccaacccct tgtgacgaat
     2281 gcgctcagtg caaggaaatc atccagggaa attgtatcga tgtcatcgaa atcgacggcg
     2341 cctccaatcg tggaattgac gaggttcgtc agatcatcga aaacgttcgc tatcaaccgg
     2401 cgcgctgcgc gttcaagatt tacatcattg atgaagtcca ccaggttact aaagacgctt
     2461 tcaacgcact gttgaaaacc cttgaggagc ctccagcttc ggtgaaattt attcttgcca
     2521 ccaccgagcc gcatcggttg ccagagacaa tcttgtctcg ttgccagcga tttgatttcc
     2581 ggcgcatatc actgaaggaa atcgttcaac ggttaagtga tatttccgct agagaaggtc
     2641 tcaagatcag ccaaggtgct ttgatagccg ttgcgcgcga agccgacgga agcatgcgag
     2701 acgctcagtc tctattggaa caagtgttat cctgcgttgg ggcagaacag caacagagcg
     2761 aattagatga aacacagcta caggagattt tagggttggc cgagaggcgg atgctgtttg
     2821 acatctcaga atcggttatc gccaacgacg gcaaacgatg cctggaattg gtggcgcagg
     2881 ctgccgagca ggggcgcgat ttaactcgcc tgtcaaaaga tttgttggag catttcagaa
     2941 atttgatggt tgcacgactg tgcggacaaa aaaaaccaga gaagctagcg gatgaggagc
     3001 gagcagaact tcttgattta ccggaccaag agatcatcgc tttgagctcc caggcggctg
     3061 acgtctctat tgataccttg ttggattatt tcgaacttat ggcagcaggg gaggacgatg
     3121 tcgcgcgcgc ggcaaatcct cgtttcactt tggagacgtc gttagtgcga ttggcgatgc
     3181 tgcctaaaac tcttccgatt attcaattga tcgaccggct cacacaactt gagaaccggc
     3241 tagtcagcga gggaaaagcg attcctgtcg gaatgaagac gctagcaccg cggacatcga
     3301 ctaccgccgg ttcaaattca atcgaagcga ccgtcgtgcc gattttaacg tctgcgctgt
     3361 cccaggataa cgagcaggtc tggcggacgt ttgtctcgtt tgtcggcaaa gagaagaaac
     3421 tattagcatc gcatctggag acgagcagct ctatcgctat tgcgcccgaa tctttgacca
     3481 taggaatcgc cgagaagcat catcttagct acctccagga tctcgagaac atggctctct
     3541 tgagaaactt tgcgaaacgt ttcttttcgc gcgagatgac ggtccaattg acccaactgg
     3601 atgcgtcgaa caccaagtct caacaggagg tgctggcaag cggggacacc ggcagcgaaa
     3661 tggtcaaaga agcgctgcga attttcggcg gctcgatcaa aggagtacgg accgataata
     3721 gttgataaat ttacgttagg tcgtttacgt ttatagcagg aggatccatg gctaagggaa
     3781 ttggcggaat gggtaattta ctcaaacaag cgcaggaaat gcagtcgcgt atggcaaaga
     3841 tccaggaaga gttggcacaa aaaaccgtgg atggatcggc cggcggtggt atggttcggg
     3901 tgacagtcaa tgggcagctt gcgcttaccg caattaaaat cgatccaacc gtaatcaatc
     3961 ccgaagaaaa ggagatgctc gaggatctga ttatggctgc aatcaatgat ggcatgcgta
     4021 aggcgcgtga catggcttct tcggagatga gtaagatcac gggagggctt aaaattccgg
     4081 gattgtccgt atgagcacgt atcccgcgcc ccttgcacgg ctggttcaag aattatcgaa
     4141 gcttcctgga atcggggaaa agacggcggc gcgcctggca ttccacatgt taaaagcgaa
     4201 gaaagatgat gtcttcaatc ttgccgatag tatcgccaag ttgcgcaaag agatggggct
     4261 ctgccggatt tgcttcgggt tcagcgaggt cgatccgcaa agcggcgaaa cgagcgtctg
     4321 catgatctgc cgaaatcctg aacgagaccg gcagagcatc tgtgtcgtcg aagagccgtc
     4381 ggatctgatc gcggtagaaa gatcgcagga attccgcggg ctctatcacg tgcttcatgg
     4441 aaccatctct ccgctggatg gtatcgggcc tgatgcgtta cggatcaaag aattgctcgt
     4501 gagaattaag agcgaagtgg ttcaggaaat tatcgtcgcc actaatccga caatggatgg
     4561 ggaggcgacc gcgctttatc tatccaaagt gatcaaaccg ctgggtgttg cggtaacccg
     4621 gattgcccgt gggctgccga tgggcggcga ccttgaatat accgatgcgg tgacgttggg
     4681 caaagctctt gaaggccgga gagagatata ggactaacat ggggcgtatc attccagcca
     4741 tttttgtttc gcttatacta tccggctgct tttatcaggg tcgcaacttc gatatctcgc
     4801 cggtgaggaa tatcagaaac gatgtgacga ctcagaggga gatttatgca gactttggtg
     4861 aacctttaag gagaggccta gaaaacggcc tggagaattg ggtgtacagt tatcaatatt
     4921 acgagctcgg gcagctgcgc gagtccaaag atctgtacgt ggtcttcaac aaggataata
     4981 ccgtcagaag ttactcgttc acatcgcgct aagttccgtg ggcggcaagc gccttcatac
     5041 cgcctgaaag agaataaaaa gttttgtcgc caaccagccg atcgcaccgc aaaccggaaa
     5101 tgtcagtatc caagcggtaa cgatttcgaa cgttaccccc caacgcacgg ccgagagcct
     5161 ccgagtggca ccgacgccga tgatggcagt gttgatggtt tgagttgtac tgaccgggat
     5221 cccgagccga gtggccacct cgattgtgac cgcagcggca gtttcggcgg cgaagccatg
     5281 aaccggttca agcttggtga ggcgcagtcc catcgtacga acaatgcgcc atccgccgac
     5341 ggcggtacct acacccatcg ttagagcaca aagcaagatc acccagattg gtaccctgaa
     5401 ttcaggtaaa attccgccaa gtaggagagc caaagtaaac acgcctataa atttctgacc
     5461 gtcgttggaa ccatgactaa acgccatgaa cgctgccgag agaatttgca ggcgtccgaa
     5521 gatgcgccga acttttcccg gtcggctgtg ggccagcgcg cggtatagaa tcgccatcag
     5581 caacaaaccc ccaaaaaagc ctaaaaagga agaaaatagc aggccaatca ggaccttgct
     5641 ccagcccgcc cagaccaggc tttcaggacc tgcggtggcg agtccggcgc ctgtcaaacc
     5701 ggcgatcaga gcatgacttt cgcttgtcgg taacccgtag taccacgcca gcgtgctcca
     5761 aacgatgatt ccaaccattg cagccgccac agtagtaaga ttgatgattt ccggacgtac
     5821 gatatctttg ccaatcgttg ccgcgaccgc ggtgcccgac atggcgccta tggcattgag
     5881 caccgttgcc atgatcagcg cctgataagg cgaaagcact cgtgtcgaaa caaccgtggc
     5941 gatagcgttc ggtgagtcgg tccacccgtt gacgaattcg gcggccagaa tgagtcccag
     6001 gaccagaaga agcaacaggt tgccgtcgag catgttgagg gctgatggag gggcttagga
     6061 gtttttcaga ccgacggtct cgagagtgtt cgctacgtct tcgcagcggt cagtagcaga
     6121 ttcaaggatt tcgtaaatgt cgagccattt gatgacgtcc agaggaggtg ttccgttttc
     6181 aaacagccga ccaatggctt tgcgataaat atcatccgca gtgttttcaa gccggttgat
     6241 ttcgatgcaa tactcgtcga tatcatccaa tcgcggcatg tgtgaaaccg ctttaacgag
     6301 ttgttctgtg gatgtcacga tgatgtcgct cagttctcta gcttcggcgg tgggctcgtc
     6361 aatgcgaaaa aggaccatgc gcgacgccgc agcctcgatt gcgtccatca cattatccaa
     6421 cgcagtaatc agcgcgtgaa tatcttcggc gtcaatcgga gtgaggaagg tttgatttaa
     6481 gctgcggatt gtcctgtgtg taatccggtc tccttcatgc tccaggtctt taattttcct
     6541 ccaagactct tcaggattat cgtaatgttc aagcatttct ttgagcgctc gagcgccagc
     6601 cagagcattt tccgcggctt gcttgaacag atcaaaaaac ttttctttat gcggagcgaa
     6661 attgaacata ccgatggatt gctggtgaag ctttctatct agcagtagcc ttcagcaatt
     6721 gtcaagattg gttctgcatt gtggcattcg tgagcagcta gtacgaggat ataatgaaaa
     6781 actgctgaac gggtgctcaa ttatcattac acaccgccct caatacttga agaactttgt
     6841 tcatcatcac ctttggcgag cactggatct attaacgggc aatgcattga ttgcacctta
     6901 atcggaaagg tcgcacagtt ttcggcggca ggtatcgcaa agcgcaataa ataatccgac
     6961 ttcgcgatgg gtacctcctc gccgccctag agtattgatg cgggatgtcg atcgacttct
     7021 ttcatgactc ttacctcaac ggatgaatga gtttgggtcc ggtgcaaacg ctatagattc
     7081 gtcggccctc ttcctcgcgc gccggcgaca ccttcggcga tcttgctgcg gcacgagttt
     7141 tccggtgcaa aaaaacagga ttgtgtttgt gttggattcg gtctcactgc cgcagcagaa
     7201 cgcgaattgt cgtgcccttc tgaaggatgc tctcgatctt taggtcggcc tgatgtgcct
     7261 gcgcaatatg tttaacgatg gctagaccca atccggtgcc gccaatatct cgggatcgag
     7321 ccttgtcgac acggtagaat cgttccgtga gcctgggaat atccttctcg gggattccgg
     7381 ggcccgtgtc ggatactgac acttccactc cgccggcatt gccattgatc ctagttcgtt
     7441 gaactgtgac ggttaccgtg ccgttggttg gagtatactt cactgcatta tccacgagat
     7501 tgataaaaag ctgctgcaaa cgatcgaagt cgccactcac cctgggcaat tcagggtcta
     7561 cgaagctctc tagcttgatc cctttcttgt tggcc
//
LOCUS       DASUTG010000616         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1577494, whole genome shotgun sequence.
ACCESSION   DASUTG010000616 DASUTG010000000
VERSION     DASUTG010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577494"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1098
                     /locus_tag="VF089_14270"
     CDS             349..1098
                     /locus_tag="VF089_14270"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175174.1"
                     /translation="MAAIDNKVAIITGSGGGIGGAIALRYAREGARLALADIVAETMK
                     SRAGEINSMGCDALAIATDVTNKDSVRQMVHTTIERWGRIDILVNVAGGADRKPVLEM
                     SEADWDHIIDMNLKSVFLCCQAVLPAMLRQKYGKIVSISSIYGFTGNATRSSYAAAKA
                     GVAVFTKSLALEVAKEGISVNAIAPGRISTPRVRGHYSDEQWTEAMARIPAGRAGTPD
                     EVASTALFLVLDENRYITGQTIHVNGAWLNW"
     gene            1274..1999
                     /locus_tag="VF089_14275"
     CDS             1274..1999
                     /locus_tag="VF089_14275"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175175.1"
                     /translation="MYGWRAKIGHVAPSRGDTLVYEFYQMMPEGFMILNSTGTIRQLV
                     DADFERQLQRIEEAAQDLVENKCDAIIIGGSPLFTKLGYGSDIEMAKKLTARFGVPTS
                     PGITGEIAALRSLNIKKLVVATPHEDSLNDRMKAFLEASGFNVLKIQGYGVRKNADLT
                     DMDIHAAYRIAKKLYEQASEADGIFVPCPRWPTITDVDLLEREIGKPVVTSCQAYIWH
                     ALRLAKVKEGIEGFGRLMASLAN"
     gene            complement(2052..>2660)
                     /locus_tag="VF089_14280"
     CDS             complement(2052..>2660)
                     /locus_tag="VF089_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175176.1"
                     /translation="YLQDVAEKYTGLDISPSVARFYHKKFVLGSATAMPFADDSFDGV
                     WSIWVFEHVPNPEQAFREARRVTRDNGVLFLLPAWDCTPWAAEGYPVRPYSDFTAAGK
                     IIKASIPLRASPPFLAMSKLPNRLVRNLVAQFGPTKLRYRRLTPNYEKYWMPDSDAVN
                     SIDRYEAMLWFRSRGDECLNCNDAALSIAPMPLIIRVHKSAT"
BASE COUNT          640 a          698 c          698 g          624 t
ORIGIN      
        1 acattgggcc tgccctcttc gacttcttga tattcgacgt gcatgccgag tcgctcgaat
       61 tcgcttccta aatattctgc gacttctttt tcctctccgg tgatgctcac cgtgtcagcc
      121 agcttgcagg cgatttcgac aattcgctga cgatccacct gctcgagcac ttgttgcatt
      181 cacttctcct ctcgtttaat tgtaacgcgc ttataaacag aacgtcttta acacagtggt
      241 gttattgctg acaacgatcg tattgacgga ccagcacaat tcacctagat tcgtcaggtc
      301 ttctcggctt gcacacttca tcagcttcga tgaattggcc agtatcgtat ggctgccata
      361 gacaataaag tagcgattat taccggtagc ggcggcggaa tcggcggtgc tattgccctg
      421 cgttatgcac gggaaggcgc cagactcgct ctcgccgaca tcgttgccga gacgatgaaa
      481 agtcgcgccg gcgagattaa ctccatgggt tgtgacgcac ttgccatagc cacggatgtc
      541 accaacaaag actcggtgcg gcaaatggtt cacaccacga tagaacggtg ggggcggatc
      601 gacatcttag tcaacgtcgc cggcggcgcg gatcgcaagc cggtgctgga gatgtccgaa
      661 gccgattggg accacatcat tgatatgaac ctaaaatccg tcttcctctg ctgtcaagcg
      721 gttttgccgg cgatgttgag acagaaatat ggaaagatcg ttagcatctc gtcgatttat
      781 ggtttcaccg ggaacgccac ccgttcaagc tatgcggcgg caaaagccgg cgttgctgtg
      841 tttaccaagt ccctggctct tgaggtggcc aaggaaggta ttagtgtgaa tgccatcgct
      901 cccgggagga tttccacacc gagggtgcgc ggccactatt cagacgagca gtggacagaa
      961 gcgatggcgc ggattcccgc cggccgtgcc ggaaccccgg acgaggttgc cagtactgcc
     1021 ctttttcttg ttttagacga gaatcgctac atcacgggtc agacaattca tgttaacggc
     1081 gcctggctca actggtagca ggctgttgaa aaaggctcac ctgcgtcgtt gcgctcgccc
     1141 gactcgcttc aacgtactaa ccgtacgcct cagctcatcg atccatcgcg cgccttgcat
     1201 ctgagacctt tttgaacagc ctgagatacg atatcgtaga cgacaaacta ctcgtaaact
     1261 gagggaggaa attatgtacg gttggcgcgc caagataggt catgtcgctc cgtcgcgtgg
     1321 cgatactctg gtgtatgaat tctatcagat gatgccggaa ggcttcatga tactaaacag
     1381 cacaggaaca atccgtcagt tagtggatgc agattttgaa cgacagttgc aacgcatcga
     1441 agaggcggcc caggatctcg tcgagaataa atgtgacgcg atcatcatcg gcggctcgcc
     1501 attgtttacc aaactcggct acggcagcga catcgaaatg gcgaagaagt taaccgccag
     1561 attcggcgtc ccgacttctc cgggaatcac cggcgagatc gcggcgctca gatctctcaa
     1621 catcaaaaag ctcgtggtcg ccacgcccca tgaagattcg ctcaacgatc ggatgaaggc
     1681 atttctggag gcatcaggtt tcaacgtact gaagatccag ggttatggag tgcgcaagaa
     1741 tgctgatctc acggacatgg atattcatgc cgcctaccgt atcgccaaga aactctacga
     1801 acaggccagc gaagcagacg gcatattcgt cccctgcccg cgctggccga ctatcacgga
     1861 tgtcgattta ctggaacgcg agatcggcaa gccagttgta accagttgtc aagcatacat
     1921 ctggcatgcc ttgagattgg cgaaggtcaa agaaggtatc gaaggcttcg gacgattgat
     1981 ggcttcactg gcaaattgac ttgcttttcg acacagcaga ttagatcagt accgtggcgt
     2041 ttgggttaaa cctatgtggc tgatttatga acacgtatga tcagaggcat gggagctata
     2101 gataacgctg cgtcgttaca gtttaagcac tcatcacctc tgctacgaaa ccatagcatc
     2161 gcttcatatc gatcgattga gtttaccgca tcgctgtccg gcatccagta cttctcgtaa
     2221 ttaggcgtca gccgacgata gcgcaatttt gtcgggccga attgagcgac aaggtttcga
     2281 accagccggt tcggcaattt gctcattgct agaaaaggcg gtgacgcgcg cagtggtatg
     2341 cttgctttga tgattttgcc agcggcagtg aaatcagaat agggcctgac cggataaccc
     2401 tcggcagccc aaggggtaca gtcccaagcc ggtaatagga agagtactcc gttgtctcgt
     2461 gttacacgcc gtgcctcacg aaacgcctgc tccggattag gaacatgctc gaacacccaa
     2521 attgaccaaa ccccgtcgaa actatcgtct gcaaacggca ttgccgtagc cgagccaaga
     2581 acaaatttct tgtggtagaa acgcgctaca ctgggagaga tatccagccc ggtatatttt
     2641 tctgcgacgt cctgcaaata
//
LOCUS       DASUTG010000617         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_986870, whole genome shotgun sequence.
ACCESSION   DASUTG010000617 DASUTG010000000
VERSION     DASUTG010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986870"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VF089_14285"
     CDS             complement(<1..1010)
                     /locus_tag="VF089_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175177.1"
                     /translation="MTVLRMMKALGVLTLCQSLLFILIVTRIDAATIAEVTAKVSTLN
                     PQDKQNFLVKGAQKEGELVYYGTILVNEFSQLGKAFNARYPFLNLKHYYAPREGILNR
                     SLTEARAGSHAVDAIQVDSSYGYQLVNENLVHPYASAARERFYDGTYDSNGSWHSMYY
                     LTTALIYNTTLVKPESVPQTYDALLDPAWKGKLLFDPEAGYILAAMEQAWGREKALDY
                     LTRLRKQDLSFRRGGALTTQVVSSGEYPIGIAINGETSAAIRDKGAPLGFKVLSPKIV
                     KPEGLFLARNSPHPHAALLFAEWVLSEEGQSILAGKLGKGVAMKGVRSKFQEFQLQPD
                     YVV"
     gene            complement(1139..2464)
                     /locus_tag="VF089_14290"
     CDS             complement(1139..2464)
                     /locus_tag="VF089_14290"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6175178.1"
                     /translation="MSARVKPMRLDLAEFPVKEIHLGKRFHYQAGILEVDQQALTDLV
                     LEDRRIEATSLEVVYPGERVRVTGIRDIVEPRVKVGCQRQVFPGTLSPVEHVGEGLTH
                     RLSGMAVIATAAYEGTVRAGLAVQRSALLDMWGPGADASRFSSLIGLVLVMRLKQALS
                     DLDAHQAIQGAELRVAQRLAEATVGRPPEDSELFELGETRRDLPPVVLIQGCLTDSHN
                     LHSGVSYYGLPIRDSLATFVHPNELLDGAITVTATRAVGHFPITWDWQNHPLALGLCR
                     QHGKQINFLGVILERIQFDTFLGKEVAAHNASQLAATLGATGALVAWIGSGNAFVELM
                     LTIRACEQRGIKTVLITYEFGGKDGVDSPLLYYLPQADAVVSSGSRDRWLELPAPERV
                     VGPYKEFSILTYPGAPVVPAMGEITLDARDMLIGGVDNWGRQSWRCNAY"
     gene            complement(2448..3434)
                     /locus_tag="VF089_14295"
     CDS             complement(2448..3434)
                     /locus_tag="VF089_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175179.1"
                     /translation="MRLRISLTIALLLAATHVQAATRTVISYASFNERTAGALLVAQD
                     QGFFRKQDLDARLVYVRAGNVALSALAAGESQFYFGSGTGVTLGAVAGGLDGVFVAGL
                     VNRLTGTFVVHPQIKNPADLKGRNIGVQSIGGGIWMITMLVLDHWGIEPKRDGINLRI
                     VGDEAVLAQALAANLIEGTYLGYTFGSLMKRQGFSVLADLADLNIPYQSTGVFVRRSF
                     LKSAPDTVEKVLRAMTQATAYIEKPENKNSVMTSLYKGLRLPRFQDAEEGYELMRTLY
                     ERRIYPNADGIRNAIRLLGTSNEKIRQLKAEDLVDDRLVRKLEKEGVFERPR"
BASE COUNT          801 a         1073 c          906 g          792 t
ORIGIN      
        1 acgacataat cgggctgtaa ttgaaactcc tgaaatttgc tgcgcacacc tttcatcgcc
       61 actcctttgc cgagcttgcc ggctaatatg ctttgcccct cttccgaaag aacccactcg
      121 gcgaaaagca atgccgcatg cggatgcggc gagttcctgg caagaaacaa accctccggc
      181 ttaacgatct tcggcgataa tactttgaat cccaacggcg cccccttatc tcgtatggcc
      241 gcggaggttt cgccattgat ggcaatgccg ataggatact cgcctgaact caccacctgc
      301 gtggtcaaag cgccgccgcg cctgaagctg agatcctgct tccgcagcct tgtcagatag
      361 tccaacgcct tctcccgtcc ccatgcctgc tccatcgctg ccagaatata gccggcttcc
      421 ggatcgaaca gcagtttgcc tttccacgcc ggatcgagga gcgcgtcata ggtttgcggc
      481 accgactccg gcttgaccaa agtcgtgttg taaatcaatg cggtggttaa ataatacatg
      541 ctgtgccacg agccgttcga atcgtacgta ccgtcgtaaa atcgctcccg agcggcgctg
      601 gcgtaaggat gaactaaatt ttcattaacg agctggtaac cgtagctcga atcgacctgg
      661 atcgcgtcca cagcatggct gccggcccgc gcctcagtaa gggagcggtt aagaatcccc
      721 tcgcgtgggg cgtagtagtg ttttaaattc aagaacggat agcgagcgtt gaaagccttg
      781 ccgagttggc tgaattcatt gactagtatg gttccgtaat aaaccagctc gccttccttt
      841 tgagctcctt tgactagaaa attctgtttg tcctgaggat ttagcgtgct gactttcgcc
      901 gtcacctccg cgatagtcgc ggcgtcaatc cgggtcacaa taagaataaa cagtaatgac
      961 tggcatagcg ttaaaactcc gagggctttc atcattcgaa gcaccgtcat aacctgcctt
     1021 tcagtcgcct aattggtatc gatccagaat catgcaaggt tcacccaaag accgttcgtc
     1081 agcggaaacc ttctgtattc tcccttggat tttacgtcga ttgaattgcg gaaccgcatc
     1141 aataagcatt gcatcgccag ctttgacgtc cccaattatc aacgccgccg atgagcatgt
     1201 cgcgcgcgtc cagagttatt tctcccatcg ccggcacaac cggcgctcca gggtaggtca
     1261 aaatcgaaaa ttccttgtac ggtccgacca cacgctcggg cgcgggaagt tcgagccagc
     1321 gatctctgct gccgctgctg actaccgcat ccgcctgcgg cagataatag agcaagggcg
     1381 aatccacgcc gtctttgccg ccgaactcgt aggtgatcag cacggttttg atgccgcgct
     1441 gttcgcaagc ccgaatcgtc agcatcaatt caacgaatgc attgccggaa ccaatccacg
     1501 caaccagcgc gcctgtggcg ccgagggtcg ctgccagctg agatgcgttg tgcgccgcca
     1561 cctcttttcc caagaaagtg tcaaattgaa tgcgttcgag tatcacgccg agaaaattaa
     1621 tctgcttgcc gtgctggcga cacagtccaa gtgccagagg atgattttgc cagtcccagg
     1681 taattggaaa atggccgaca gcccgagtgg cagtcacggt gattgcgcca tcgagcagtt
     1741 cgttaggatg cacaaacgtc gccagtgagt cgcgaatcgg caagccgtag taactcacgc
     1801 ccgagtgaag gttatggcta tctgtgagac aaccttgaat caacaccact ggcggcaagt
     1861 cccgccgcgt ttcgccgagc tcgaagagtt cgctatcttc tggcggccgg ccgacggtgg
     1921 cttccgcaag ccgctgcgcc acccttagct ccgcgccttg gatcgcctga tgcgcgtcca
     1981 aatcggacag cgcttgctta agccgcatca ccagcaccag cccgatcagg gaactaaacc
     2041 gcgaagcatc cgcgccaggt ccccacatat ccaggagcgc gcttctctgc accgctaggc
     2101 cagcgcggac cgttccctcg taagcggcgg tcgcgatcac tgccattccg gagagacgat
     2161 gcgtcaggcc ctcaccgaca tgctctaccg ggcttagcgt acccggaaat acctgacgtt
     2221 ggcagcccac tttcacgcgc ggttcgacaa tgtctcggat tcctgtcacc cggactctct
     2281 cgcccggata gaccacctcc agggatgttg cttcgatgcg gcggtcctcc aagacaagat
     2341 ccgtcaaggc ttgctgatcc acttctaaaa tcccggcctg ataatgaaac cttttcccca
     2401 ggtgaatctc tttcaccggg aactcggcga gatctagccg catcggttta acgcgggcgc
     2461 tcaaagacac cctccttttc taacttccgg acaagccgat catccaccag gtcctcggct
     2521 ttgagctggc ggattttttc gttgctggtt ccgagcaagc ggatggcatt gcggatgccg
     2581 tcagcattcg gataaatgcg ccgctcataa agcgtccgca tgagctcata tccttcttcc
     2641 gcatcctgga agcgcggtaa gcggagtcct ttgtaaagac tggtcatgac actgttcttg
     2701 ttttccggct tctcgatgta tgccgtggcc tgggtcatcg cccggagcac cttttccact
     2761 gtgtctggtg cggatttgag aaagctccgg cgcacgaaca cgccagtgct ttgataggga
     2821 atattcaaat cggcgagatc ggcgaggaca ctaaatccct ggcgtttcat aagcgatccg
     2881 aaggtatagc ccaagtaagt accctcaatc aggttggcag ccagcgcctg cgccagaacc
     2941 gcttcgtcgc cgacgattct caagttaatg ccgtcacgct tgggctcgat gccccagtga
     3001 tccaacacca gcatcgtaat catccagatg ccgccgccga tgctctgcac gccgatgttc
     3061 cggcctttca aatctgcggg attttttatc tgcggatgaa cgacaaaagt accggtaagc
     3121 cgattgacca atccggcgac gaagacaccg tccaagccgc cggccaccgc accaagcgta
     3181 acaccggttc ccgaaccgaa ataaaattgt gattcacccg cggctaacgc cgacagcgca
     3241 acgttcccgg cccggacata caccaggcgc gcgtccagat cctgtttgcg gaaaaaaccc
     3301 tggtcctgag cgactagaag agcgcccgcg gtccgttcat tgaaactggc gtatgaaatc
     3361 accgtccggg tagccgcttg gacatgtgta gctgcaagta gcagcgcaat cgtgagactt
     3421 attcgaagac gcatgatcaa cctccacgat ccttatacta atagtgttcc atggttcaaa
     3481 cgttcaacag ttccacgtca gaagctgcaa tggttccaac agttccaaac gttccaactc
     3541 gctaaaacca gattggctcg gcggcttctt cg
//
LOCUS       DASUTG010000618         3616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_540444, whole genome shotgun sequence.
ACCESSION   DASUTG010000618 DASUTG010000000
VERSION     DASUTG010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540444"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(779..2461)
                     /gene="ettA"
                     /locus_tag="VF089_14300"
     CDS             complement(779..2461)
                     /gene="ettA"
                     /locus_tag="VF089_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX6175180.1"
                     /translation="MSTEPNKVIYSMVGVSKYYDKKAVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLRILAGVDKEFNGEAILSPGYTVGFLEQEPLIAEARTVREVVEEGVQETVD
                     LLKEFEEISAKFAEEMSDDEMSALLERQGEVQEKLDHQDAWDLDARLEMAMDALRCPP
                     PETPVKVLSGGERRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQSYPGTVI
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYTSWLEQKQERLRRQEKSESERQKTLQR
                     ELEWIRMSPKARRAKGKARINAYDALLEQESQKRREDLDLHIPPGPRLGNTVIEAEGV
                     SKAYGDHLLVEDMSFSLPPGGIIGVIGPNGAGKTTLFRMITGEEQPDSGTIRIGETVV
                     LGYVDQSRILNPEKSIWEEITGGADLLKLGAREMNSRAYVARFNFSGTDQQKKVGRLS
                     GGERNRVHLAKMLKGGVNVLLLDEPTNDLDVNTLRALEEALENFAGCAVVISHDRWFL
                     DRIATHILAFEGDSKVVWFDGNYSEYEADRKQRLGAAAEQPHRIKYRPLTRG"
     gene            2520..3545
                     /locus_tag="VF089_14305"
     CDS             2520..3545
                     /locus_tag="VF089_14305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175181.1"
                     /translation="MQEIIKSGDDVMGTNMKFGLLLPHFCEHASVADCLEGAQRAESY
                     GFDSVWVRDHLVFEPHGIEGSDNTHIEGLLLLSAIAAVTNSITLGTSMAICHRHPIYL
                     AQLFAGLSVISNGRIIMGIGLGGFPHEFAAAGYPTGIEERAELVRSNVQICRRLWAGE
                     TVSFEDEYFQFADVPLKPCPVRPIPIWYGGGTPAACRRAVEYCDGWMPARTTLPTFEK
                     LIVYLRKLCQNANKPMISAAVMPFTSVHKDREMALSKISIESLLREANKFPTWVKPAG
                     GVFSRLDDIRGLILAGTPADIVRETRAYEAAGADHIVYDLRFRYADWHEQVDLLGQEV
                     LPALRHR"
BASE COUNT          791 a         1012 c          977 g          836 t
ORIGIN      
        1 ccttgataga acggcgcttg ggcttgcagt tcggtaaccg aagcgatcag aatagaaagc
       61 gatagcagca acttcttcac atgacctcct agcacttcaa agttggcctg agcgccgcat
      121 actaaatcga agtttctcgc aacagcaagc aaaaaaattt cctgtcgcga gtcgctaatc
      181 agtaggcgga agactgctag aggcttttgg agaaccaaac ccggggatcc gaagcctcat
      241 ccggcccggt gcttgtcggt ttgcggatta tcgtttcgtc attctctcaa ccaatgttgc
      301 tctagtgcgt tccttgtccg tgtttcgaat gaattcccat gcggagaatt gtgacgcgct
      361 acttcggcat ctgcttgcaa acggcctcat atagaaaaag gctgaagcgc acaagatgaa
      421 gctattcgtt tcacccattc agatccgctg ttaatcggca caaagccgag ttgatttcga
      481 cacgatgatg atagacagac gcattgcacg acgggagagt tgcttacctt ggcggccgaa
      541 gacaattcac aaggttgcgg aatcagttac cgccgcgcga acggaccagc aggtcgtcct
      601 actcggcacg ggtagttcga aagtagcgga ggctagagat ctcgaggttc aacaggctgt
      661 tcaaaaaggt ccacatgcga ggcgcgcgat gaatcgatga gcggaagcgt acttttagta
      721 cgttgacgcg agacgattga gcgcaacgaa gcaggtgggc ctttttggac agcctgcttc
      781 atccgcgcgt caacggccga tactttattc ggtgcggttg ttccgctgcc gcgcccagcc
      841 gctgcttgcg atccgcctca tactccgaat agttgccatc gaaccaaacc actttgctgt
      901 cgccttcgaa tgccaggata tgggtggcga tgcgatcgag aaaccagcga tcatgggaaa
      961 ttaccacggc gcagccggcg aaattctcca gtgcctcctc cagagcgcgc aacgtattca
     1021 catcgagatc gttggtcggc tcgtcgagca acaacacgtt gacgccccct ttcaacatct
     1081 ttgccagatg aacgcgattg cgttcaccgc ccgacaacct ccctactttc ttttgttgat
     1141 cggtgccgga aaaattaaat cgcgccacgt acgcccgcga gttcatctcg cgcgccccga
     1201 gcttcagtaa atccgccccg ccagtgattt cttcccagat cgatttttcc ggattgagga
     1261 ttcggctctg atcgacgtat ccgaggacta ccgtttcacc gatgcgaatc gttccgctgt
     1321 cgggttgttc ctctcctgta atcatccgaa acaaggtcgt cttgccggcg ccgttcggcc
     1381 cgatgacgcc gatgatgccg cccggaggga gcgaaaagct catatcctct accagcaggt
     1441 gatcgccata agctttggaa accccctccg cttcaatgac ggtgttgccc agacgcggcc
     1501 cgggcggaat gtgaagatcg aggtcttcgc ggcgcttttg cgattcctgt tcaagcagcg
     1561 catcataggc attgatgcgc gccttgcctt tggcccgccg cgctttcggc gacatacgaa
     1621 tccactcgag ctcgcgttgc agcgtcttct gcctttcgct ttcggatttt tcctggcggc
     1681 gcaggcgctc ctgcttctgt tccagccagg atgtgtaatt tccttgccac ggaatgccct
     1741 ggccgcgatc gagctcaaga atccaacccg cgacattgtc gagaaagtat cgatcgtggg
     1801 tcactgcgat cacggttccg ggataactct gcaggtgatg ctcaagccac gccacggact
     1861 cggcatccag gtgattggta ggctcatcga gtaaaagaat atcaggcttc tgcagaagca
     1921 agcggcacaa ggcgacccgg cgacgctcgc cgccggacag caccttaacc ggggtttctg
     1981 gaggcgggca gcgcagcgca tccatggcca tctccagccg tgcatcaaga tcccacgcat
     2041 cttgatgatc gagcttctcc tgcacctcac cctgccgctc gagcagcgcg ctcatttcgt
     2101 cgtcggacat ctcttcggcg aatttagcgc tgatttcttc gaactctttc aacagatcaa
     2161 ccgtctcttg aacaccttct tcgacaactt cgcgcactgt ccgcgcctca gcgatcaagg
     2221 gttcctgctc aagaaagcca acggtgtagc ccggagagag tatcgcctcg ccattgaact
     2281 ccttgtcaac tccagcgagt atgcgcagca gcgaggattt gccggatccg ttgagaccga
     2341 tcacgccgat cttggcgcca taaaaataag agagatagat atctttcagg acggccttct
     2401 tgtcgtaata cttgctgacg ccgaccatcg aatagatgac cttattgggt tccgtgctca
     2461 ttgatttaaa tcatttcccc tgaaagtaag tttttcaagc tagcctgtca ggttggattg
     2521 tgcaagaaat aatcaaatcg ggtgatgacg ttatggggac aaacatgaag ttcggcttgc
     2581 tgctgccgca tttctgcgag cacgcctctg tggcagactg ccttgaaggc gcgcagcgcg
     2641 ccgagtccta tggcttcgac tctgtttggg tgcgggatca cctggtgttc gaaccgcacg
     2701 ggatcgaggg cagcgacaat actcatattg aaggattatt gctgctttcg gcgattgccg
     2761 ccgtaacgaa cagcataacc ctgggcactt cgatggcgat ttgccatcgc cacccgatct
     2821 atctagcgca actcttcgcc ggcttgagcg tgatttcaaa cggtcggatc atcatgggca
     2881 tcggtttggg cggattcccg cacgagtttg ccgcggccgg ttatccaacg ggtatcgaag
     2941 aacgggccga attggttaga agcaacgtgc aaatttgccg gcggttgtgg gctggtgaaa
     3001 ccgtgtcttt tgaagatgaa tattttcagt ttgctgacgt gccgctaaag ccgtgtccgg
     3061 ttcgcccgat tccaatctgg tacggcggtg gcacaccggc cgcttgtcgg cgcgccgtcg
     3121 agtactgcga cggctggatg ccggcccgga ccaccctgcc gaccttcgaa aaactcatcg
     3181 tttatcttcg gaagctctgc cagaacgcga acaaaccgat gatcagcgcg gcggtgatgc
     3241 cgtttaccag cgtccataag gatcgcgaga tggcgctgag caagataagc atcgagtcat
     3301 tgctccggga ggccaataaa tttccaacct gggtcaaacc ggcgggcgga gtgttttcaa
     3361 gattggatga tatccgtggt ttaatcctgg cgggaacgcc ggcggatata gtccgcgaga
     3421 cgcgcgccta tgaggccgca ggcgccgatc acatcgttta tgacctgcgt tttcgctacg
     3481 cagattggca cgagcaagtg gacctgttgg gtcaagaagt cttgccagcc ttaagacacc
     3541 gctgaaaaag acccatgcgt ttcgttgcgc ttaaattctc ggatcaacgt actacctgta
     3601 aagcgcaggg agcaaa
//
LOCUS       DASUTG010000619         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_392799, whole genome shotgun sequence.
ACCESSION   DASUTG010000619 DASUTG010000000
VERSION     DASUTG010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392799"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF089_14310"
     CDS             complement(<1..559)
                     /locus_tag="VF089_14310"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6175182.1"
                     /translation="MSGALAGIKIIEIASYVTGPFASQLLADMGADVVKIEEPKRGDP
                     FRGWGEKNYSATFCSLNRNKRSLTLDLRHEQAREIVLKLAAQSDVLIQNFRPGVMEKR
                     GLGYEDIRKINSKLVYCSISGFGPKGPYRDMPGYDTIGQARSGLLSLLTDPGKPQGMG
                     ISFSDHLTGMYACYGVLAALMNRMLT"
     gene            complement(655..>3194)
                     /locus_tag="VF089_14315"
     CDS             complement(655..>3194)
                     /locus_tag="VF089_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6175183.1"
                     /translation="ILAFVPSDFQNWSGETAFTKAMQGGLYIGPVRTSDRAEPYIIIA
                     VPLMESPRKTIGVLVAHVNLRFLWEVIAGSRFRRGGYAYLIDENGDVIAHEDASLVLK
                     RVNLRKLDKVQRFLAIRASDPAPAEVGPGINGAQVLSTYAEVPDLRWAVIVEQPTAQA
                     LSDLERLHRYSILLIGIGLLVGSGIIVWVSRKISRPIQELRQAVRTIRAGNLEHRAEI
                     TTGDEVEDLAQEFNDMTEALQSSYATLEQKVKQRTEETGALYEVTTAVNKSLDLQTIL
                     QAVVDKITDIFQFETTRVFLFNAEMEQMELRASFEVDPEHWTEVHTFKRGQGVIGRVV
                     ESGEPMIFENIETDPRYAALSTTRATQNAKLRFFGVLPIKTQSCVFGAIIFNGKSPRK
                     LTTDQIRLLTSMAEHLGVAVEKVNLFDEVNTRSRHLTVLHTIGAAVSRSLNLETVLNE
                     AVRKISETLNFDAVWIYELEPEKGVLHLRGQIGLSDEIARGMAERNVESGISGEVMRT
                     GRSLVFEDVLNDASYRELTSAGKIISLGFKAAAAFPIMVKENIAGTLHVVSRAKRHFS
                     QDERQLIQSIAQEIGVAVENARLFAEVKKTTSELEKTNQELLDATRAKSEFISAMSHE
                     LRTPLHVIIGNADLAHDEFFGPLNSDQKTALQKILRNSRVLLKMINDVLSLSCIEARK
                     MSLDLATVEVEELIEHAQHQVEQINRDGRLEVSWDIDETVPPITTDPIKLEEILQNLI
                     ANACKFTPKGRIDVRVRNLRERDRVQFSVADTGIGIESEDVRRIFQEFEQIKEAHTGQ
                     FDGVGLGLSIVKKYLDLLNGEIRVESEPGHGSIFTFTIPRSLRRHAA"
BASE COUNT          714 a          844 c          825 g          811 t
ORIGIN      
        1 cagtcagcat tcgattcatt agagctgcca aaacgccgta gcatgcatac atacccgtca
       61 agtgatccga aaaggaaatc cccattcctt gaggcttacc cggatcagta agtaaactga
      121 gcaacccgct tcgcgcctgg ccaatggtgt cataacccgg catatcccgg taaggtcctt
      181 tggggccaaa gccagagatt gagcagtaga cgagcttgga attgatttta cggatgtcct
      241 catagccgag cccgcgtttt tccatcaccc ccgggcgaaa attttggatc aagacatcgc
      301 tttgagcggc tagcttaaga acaatctccc gggcttgctc atggcgcaga tccaaagtta
      361 agcttctttt gtttctgttg agactacaaa atgtggcgga gtagttcttt tcgccccacc
      421 cacggaatgg atcaccacgt ttaggctcct caatcttaac gacatcggct cccatatcgg
      481 ctaggagttg ggaagcgaag gggcccgtca cataacttgc aatttcaatg atcttgattc
      541 cagctaaagc gccgctcatt gataactttc cgtcgctgac ataaatcggc tgatggttgg
      601 gtatcatctt cctaactgcg gacggcctat gaagtcaagg gtgagcgaag cccctcaggc
      661 cgcatggcgc cgcagcgatc gtgggatcgt aaaggtaaag atggacccgt ggccgggttc
      721 actctcaacc cgaatctcac catttagtaa atcgaggtat ttcttgacga tgctgagacc
      781 cagacctacg ccatcaaatt gcccggtgtg agcctctttg atttgctcga actcctggaa
      841 tatccggcga acatcttcgc tctcaatgcc aataccggtg tctgccacgc tgaactgaac
      901 acggtctctc tcacggagat tgcgcactcg gacatcgatt cggcctttgg gtgtgaattt
      961 gcaggcatta gcaatcaggt tttgcagaat ctcctcgagc ttgattggat cagtagttat
     1021 cggcgggacg gtttcatcga tatcccagct gacctctaag cggccgtctc gattgatttg
     1081 ctcgacctga tgttgcgcat gctcgatcag ttcttccact tccacggttg cgaggtccag
     1141 cgacatcttc cttgcttcga tgcacgacag gctcagcaca tcattgatca tctttagcaa
     1201 gacacgggag ttccgcagaa tcttttgaag ggctgttttc tgatcagagt taagcggccc
     1261 gaagaactcg tcgtgggcca ggtcggcatt gccaatgatg acgtgtagtg gcgttcgaag
     1321 ttcatgtgac attgccgaga tgaactctga ttttgcgcgt gtcgcgtcga gaagctcttg
     1381 attggttttc tcgagctcgg aagtggtttt cttgacttcg gcaaataatc tggcgttttc
     1441 cacggcaacg ccgatttcct gggcaatgga ttggatgagt tgtcgttcat cttgggagaa
     1501 gtgacgcttg gcgcgactga ccacatgtaa agttccggca atgttctctt tgaccatgat
     1561 cggaaacgcc gcggcggctt tgaatcccag cgaaataatc ttaccggcac tggttaactc
     1621 gcgataactg gcgtcgttga gaacgtcctc gaagacaagc gagcggccag ttctcattac
     1681 ctccccgctg attccactct ccacattgcg ctcggccatg cctctggcga tttcatcgct
     1741 taagccgatt tgtccccgta gatgcagaac ccccttttcc ggctccagtt cgtaaatcca
     1801 tactgcgtcg aaattgagtg tctctgaaat cttcctgaca gcttcgttga gaacggtttc
     1861 gagattgagc gaacggctga cggctgcgcc gatggtgtgt agcacggtca gatgccgcga
     1921 cctcgtgtta acctcatcga ataagtttac cttctctacc gcgacgccga gatgctctgc
     1981 catggaagtc agcaggcgaa tctgatcggt agtcagtttt ctaggcgatt tgccattgaa
     2041 gatgatggca ccgaaaacgc aggactgggt cttgatcggc agtacgccga agaaacgcag
     2101 cttggcgttc tgcgtggcac gagtggtgct gagagcagca tagcgggggt cggtttcaat
     2161 gttttcaaat atcattggct cgccggactc gacgactcgg ccaatgacac cctggcctct
     2221 tttgaaggta tgcacctcgg tccagtgctc cgggtcgact tcgaacgaag ctcgcaattc
     2281 catttgctcc atttccgcgt tgaaaagaaa aactcgagtg gtttcaaatt ggaaaatatc
     2341 ggtaatctta tcgacgacgg cctgcaggat tgtttgtaga tcaagcgatt tgtttaccgc
     2401 ggtcgtcact tcataaaggg cgcccgtctc ctctgttcgc tgctttacct tctgttccaa
     2461 cgtcgcatac gagctctgta gggcttcggt catatcgttg aattcctgcg ccaggtcttc
     2521 cacttcatcg cccgtcgtga tctcagctct gtgctcaaga ttgcccgcgc ggatggttct
     2581 gacggcctga cggagttctt gaatcggtcg actaatcttg cgactgaccc atacaatgat
     2641 tcccgaaccg actagaagtc cgatgccgat tagcaagata gagtaacggt gcaatcgctc
     2701 cagatcggat aaagcttgag cagtaggctg ctccacgatt accgcccatc gcaggtccgg
     2761 tacttcggcg taggtgctta acacttgagc gccgttaata ccggggccaa cttcggctgg
     2821 agcgggatcg ctggctcgga ttgcaaggaa ccgttgcact ttatcgagct tgcgaagatt
     2881 aacacgttta agcacaagtg aagcatcctc gtgtgctatc acgtcgccat tctcgtcgat
     2941 caagtaggcg tagccgccgc ggcggaaacg gcttccggcg atcacttccc agagaaaccg
     3001 aagattaaca tgggcgacta gaacaccgat ggttttccgc ggactttcca tcaacggcac
     3061 cgcgataata atgtagggct cagcccggtc tgaagtgcgg accgggccta tatagagtcc
     3121 gccctgcata gcctttgtaa aagctgtttc gccgctccag ttttggaaat ccgaaggtac
     3181 aaaggcaaga atct
//
LOCUS       DASUTG010000620         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1132867, whole genome shotgun sequence.
ACCESSION   DASUTG010000620 DASUTG010000000
VERSION     DASUTG010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132867"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1010)
                     /locus_tag="VF089_14320"
     CDS             complement(12..1010)
                     /locus_tag="VF089_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX6175184.1"
                     /translation="MLITWLQFILCAAIIAFAGNRLTRYGDIIAEKTGLGRTWIGIVL
                     LATVTSLPELFTGVSSVVVFDVPDIAAGNVVGACMLNMLTIAFLDLIGGSTPVSTRAH
                     QGHVLSAGISILMLGVVGLGLASGQAFGAIAWIGTYSFVLFGIYLVAMKALFSYEKRR
                     VAEFVKEFEEAQHEKISKTQAYGSFAIYALLIVAAAIFLPFIGDKIAKETGLAESFVG
                     SVFIAIATTLPELTVSYAAYRIGAVDMAVGNLFGSNLFNINILALDDLFYTHGPLLDY
                     VSGNHVVSVIGTMAMTAVAIIALTLRSAKKRFLLSWDAMTIVAVYVLIAFLLFRSP"
     gene            1127..1339
                     /locus_tag="VF089_14325"
     CDS             1127..1339
                     /locus_tag="VF089_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175185.1"
                     /translation="MIRDIEGSARDKLCDAACKAWYVDFFFKANWRPSWPPFLMTAGA
                     PSCVIGWNGLALSTVNQFASEEDRRA"
     gene            complement(1709..>2162)
                     /locus_tag="VF089_14330"
     CDS             complement(1709..>2162)
                     /locus_tag="VF089_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HEX6175186.1"
                     /translation="ARVGEGMVSTIGSAASHKAVLENPDHISTNILSRGLDAGTAYEI
                     LSIDIADVDVGQNIGAKLQIDQADADKQIAQAKAEERRAMAVALEQEMRARVVEAEAE
                     VPRAMAEAFRQGNLGIMDYYKMKNVQADTSMRDSIAETGEEPSGGQRR"
BASE COUNT          509 a          576 c          584 g          493 t
ORIGIN      
        1 tctgcatact attaaggact tcgaaacagc agaaaagcga tcagaacata aacagcgacg
       61 atggtcatgg catcccagga gagcaagaag cgttttttcg ccgatctcaa agtcagcgcg
      121 ataatcgcta ccgcagtcat cgccattgtg ccgatgacgc ttacgacgtg atttccggag
      181 acataatcca gcagcggacc gtgagtatag aaaagatcgt ctagcgcgag aatgtttata
      241 ttgaacagat tgctgccaaa gagattcccc acagccatgt cgacggcacc gatacgataa
      301 gccgcataag acacagtcag ctcaggaagt gtcgttgcga ttgcgataaa tacactcccg
      361 acaaacgact ctgcaagccc ggtttccttg gctatcttgt cgccaatgaa cggcagaaag
      421 atagccgccg ccacaatcag taaggcgtaa attgcgaacg atccgtaagc ctgcgtcttc
      481 gaaatctttt catgctgggc ttcttcgaac tctttgacga attccgcgac acgtcttttt
      541 tcataagaaa acagcgcttt catggcgact aaataaatgc cgaataatac gaaactgtat
      601 gtgccgatcc aggcaattgc gccgaacgct tgaccggagg ccagacctaa gccgaccact
      661 ccgagcatca gtattgaaat ccccgcggac aagacatgtc cctggtgcgc tcgtgtggaa
      721 accggcgttg aaccgccaat cagatcgagg aaagcgatag tcagcatgtt gagcatgcac
      781 gcgccgacga cattgcccgc cgcgatatcg ggaacatcga aaaccacgac agagctgaca
      841 ccggtgaaga gctcgggtaa ggaggtgacg gtcgccagca ggactatgcc aatccatgtt
      901 ctacctaacc cagtcttttc ggcaatgatg tcgccgtaac gcgtcagccg gtttcccgca
      961 aacgctatga tggccgcgca caagataaat tgaagccagg taatcagcat ttactactcg
     1021 ggattgattc agaacgggtt aatctgttct ccgccaatta tttaccgagg cgcgaaaatt
     1081 ataagctcgc cgcagctgaa tgcggcgata cacggagcgg tttgaaatga tccgcgatat
     1141 tgaaggctct gctcgcgata agctttgcga tgctgcttgc aaagcttggt acgtagattt
     1201 cttcttcaag gcaaactggc gtcccagctg gccaccgttt ttgatgactg ctggagcgcc
     1261 gtcgtgtgtg attgggtgga atggattggc actttcgact gttaaccaat ttgcttcgga
     1321 ggaagatcgc cgcgcctaac tgtttgaatg ttaccctcga tcatcgggcg tatcgttggc
     1381 gcggcagggc cccaagactg tcattagaat gatagctcga cgcagatcgc ggcggctgcg
     1441 gaaaagcgat ctcctgggtt gatttctcct ggacagtaaa ttctcagact gtctgagagg
     1501 aggcgctggt ggccctgccg gaacgataat ttctatgggc ggcggtttcg tgaatgtccg
     1561 aggcgcaggt tgcgcaggcg gttgcctagg tgcttggctc tcgaaacggc gctgcattcg
     1621 ctgcagcagc cagttgatca acggatacaa gatgaacgca aggaggacga gaatctgtgt
     1681 gaaagggata tcctgcatag ccaatccttt atcttctctg accgcctgat ggttcctctc
     1741 cggtttcggc aatcgagtcg cgcatcgagg tatcggcctg aacgttcttc attttgtagt
     1801 agtccatgat gcctagattc ccctggcgaa acgcctcggc catagcgcgc ggcacctctg
     1861 cctcggcttc tacgacgcgg gcgcgcatct cctgttccaa ggcgacggcc atagcgcggc
     1921 gctcttccgc cttggcctga gcgatctgct tatcggcatc ggcttggtcg atttgcagct
     1981 tggcgccgat gttctgaccg acgtcaacgt cggcgatatc gatggaaagg atctcgtagg
     2041 ccgttcccgc atccaagccc ctgcttagaa tattcgtcga aatgtgatcg ggattctcca
     2101 gcacagcctt gtgcgatgcg gccgagccga tggtgctcac catgccttcg ccgacgcgcg
     2161 cg
//
LOCUS       DASUTG010000621         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2173133, whole genome shotgun sequence.
ACCESSION   DASUTG010000621 DASUTG010000000
VERSION     DASUTG010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173133"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF089_14335"
     CDS             complement(<1..231)
                     /locus_tag="VF089_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HEX6175187.1"
                     /translation="MKISSIETIPIRLPTRRVHQWASLTTSIGVYVIIKLHTDDGRVG
                     LGEAPVLKDWGGDYGRYFGETPQSTIHIINDIL"
     gene            complement(236..1348)
                     /locus_tag="VF089_14340"
     CDS             complement(236..1348)
                     /locus_tag="VF089_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6175188.1"
                     /translation="MDQEILARLKQRIANHGLDALIAISPENVTYVSGFVVPSQSLMR
                     WRHAICIVTADGQLTMVAIDMEATTVKAHAGIDDLRIYREFTDDPMEKLAEALRDLHL
                     AGGNLAIEMEYLPARDFANLQRLLPNARWSAGDAIFNKSRQIKTVGELALLRSLSKLT
                     DKAIGDTLMQAKPGMSELELAGRLLTGLFAGGAENYKLMIIASGERSQFPNVGPSERK
                     LQKNDLIRMEIFGQKNGYLAGVCRTAVVGQESVEQQKIWANLIECKYLVMDMIKPGAR
                     CKEIYQKFLAKFSELGFEPISFVAHGIGLHLHEEPYMGRYGDEIVEAGMVGAFEPLVY
                     IPGRFGMQNKDMFCVSQRGCELMSDYTQADTLLRVG"
     gene            complement(1590..2708)
                     /locus_tag="VF089_14345"
     CDS             complement(1590..2708)
                     /locus_tag="VF089_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX6175189.1"
                     /translation="MSVAEKINSTGPKKILALDGGGIRGILTVEVLAKIESMLRRKLG
                     RGNDFVLADYFDFFAGTSTGAIIAACLSWRMSIDRIRDFYLQNGREMFDKASLLNRYY
                     RHKFEDQKLSARLRQEFGAETTLGSEKIDGLLMMVLRNASTDSPWPISNNPRAKYNDC
                     TRGDCNLNLPLWQLVRASTAAPTYFPPEVVRVGEKEFIFVDGGITMYNNPAFQAFLMA
                     TVEAFNLNWPVGESNLLLVSVGTGTSPDANADLRPDEMNVLYNASSIPSALMAAALNE
                     QDFLCRVFGKCLAGDPLDREVGDMIGTRGPVQPRLFTYLRYNAELTRHGLDGLGLPGV
                     EPKNVQQLDSIEYIADLQAVGSAVADRKVSLDHFAGFC"
     gene            complement(2721..>3898)
                     /locus_tag="VF089_14350"
     CDS             complement(2721..>3898)
                     /locus_tag="VF089_14350"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEX6175190.1"
                     /translation="KKHDDAQECIDDLLGEFPEDAESWSMRGRVEKDAWVNCWRQDNR
                     SAVDMRREAEMESATLREAINAYATGFRKDPAHYYSGINAVTLLHLLAHLSADEVKAD
                     VRTEMEGGVRWAIRGALERNPKDYWARISLAELEVLAGNKQAVDAAYKNAVAVADKDW
                     FKLNSSRQQLELLKLLGFRPEEVEAAVAVLDRALSRLNPPDKTWTPRQVLLFSGHMID
                     APDRTEPRFPADKENIAAAAIAATLEKLDAGAEDLALCGGACGGDLLFAEAALERGLK
                     LEVRLAFDEPTFLQKSVSFAPGNWTERYFKVKSHPNTTTWVMPEMLGPSPAAANPFAR
                     LNLWQLYTALAWGPEKVRLVCLWNRKGGDGPGGTQHMFETVQRYSGRVYVIDTTTLW"
BASE COUNT          848 a         1129 c         1010 g          911 t
ORIGIN      
        1 gagaatatca ttgatgatat gaatcgtgct ctgcggcgtt tccccgaaat acctgccata
       61 atcaccgccc caatctttca acaccggcgc ttcaccgagg ccaacccgac catcgtcggt
      121 gtgtaatttg atgatcacat agacaccgat cgatgtcgtc agactggccc actggtgcac
      181 cctgcgcgtc ggcaatcgaa tcgggatggt ctctatagag ctgattttca tgctctcaac
      241 cgactcttaa caacgtgtcc gcttgcgtgt agtcggacat caactcgcaa cctcgttgag
      301 aaacacagaa catatcttta ttttgcatgc cgaaacgtcc gggaatataa accagcggtt
      361 caaacgcccc taccatcccg gcttcgacga tctcgtcgcc gtaacggccc atatagggtt
      421 cctcgtggag atgcaggccg atgccatggg cgacaaaact gatcggctca aagcccaact
      481 cgctaaactt ggcgagaaac ttctggtaaa tctccttgca gcgagcgccg ggcttgatca
      541 tatccatcac cagatatttg cattcgatca ggttggccca gattttctgc tgctcgacac
      601 tttcctggcc gaccaccgca gttcggcaca cccccgccaa atagccattc ttctggccaa
      661 agatttccat gcgaatcaga tcgttttttt gcagcttgcg ttcagaaggt cctacattgg
      721 gaaattggct gcgttcgccg gacgcgatga tcatgagctt gtagttttct gcgccgccgg
      781 caaagagtcc ggtgagtagt cggccggcaa gctccagctc gctcatacca ggtttcgcct
      841 gcatcaacgt atcgccaatc gccttgtccg tcagcttgct gagtgatcgc agcagcgcaa
      901 gctcaccgac tgtttttatc tgacgggact tgttgaagat cgcatccccg gcgctccagc
      961 gagcattagg aagaagtctc tgcagattcg cgaaatctct agccggtaga tattccattt
     1021 cgatggcgag attacctccg gcgagatgga gatctcttag ggcttcggca agtttctcca
     1081 tcggatcatc ggtgaactcg cggtagatac gcagatcgtc gatgcccgca tgcgctttca
     1141 ctgtggtcgc ttccatgtcg atggcaacca tagtcagctg cccatcggcg gtgacgatac
     1201 agatcgcgtg ccgccagcgc atcaaggatt gcgagggcac gacaaatccg gagacgtagg
     1261 ttacgttttc gggagagatt gcgatcagcg cgtcgagtcc gtggttggcg atgcgctgct
     1321 tgaggcgagc gaggatttct tggtccatag tggcgctgtg aatgttcaag aaaatctttt
     1381 aatcaaatgc ccatgaatgc cccggaggat agctgctgct atccggagcg tcaagcagag
     1441 cagccgaccc cctcggcctt gattttatat ccgggcggca gtaaagaggg ctgcacagct
     1501 ttccatgcgc tatcttcaag aggcgacgac aaaaactttc cgattgcaag tttatcgata
     1561 acagctgtga gcagctaggc tctggagttt tagcaaaagc cggcaaaatg atcgagagaa
     1621 acttttctgt ccgccaccgc tgacccgacg gcttggagat cggcgatata ctcgatagag
     1681 tcaagttgct gcacgttctt gggctcgacc cctggaagtc cgaggccgtc caacccgtgc
     1741 cgcgtcaact ccgcattgta cctcagatag gtaaaaagcc tcggttggac cggtcctctc
     1801 gtgccgatca tatcgccaac ttcgcgatcg agcggatctc ccgccaggca tttaccaaac
     1861 actctgcaca aaaaatcctg ctcgttcagc gcggccgcca ttaatgccga gggtatggag
     1921 ctggcgttat aaaggacgtt catttcatcc ggtcgcaagt cggcgtttgc atccgggctg
     1981 gttccggtcc cgacagaaac aagcagcaga ttactttcgc ccactggcca gtttaggttg
     2041 aacgcctcca ccgtggccat taggaaggct tggaacgccg ggttgttgta catcgtgatg
     2101 ccgccatcga caaaaatgaa ttccttctct ccgacacgta ccacctcggg cgggaaatac
     2161 gtgggcgcgg cagtactggc acggactaac tgccacagcg gcaaattcag attgcaatcc
     2221 ccgcgcgtgc aatcgttgta tttggctcgt ggattattcg atatgggcca cggtgaatcg
     2281 gtcgaggcat tgcgcagaac catcatcagc aacccgtcga tcttttcgct tcccagtgtc
     2341 gtctcggcgc caaactcctg gcgcagccgg gcagagagtt tttgatcttc gaacttatgc
     2401 cggtagtagc gattcagtaa cgacgcttta tcgaacatct cccggccgtt ttgcaggtaa
     2461 aagtcacgaa tacgatcgat gctcatgcgc caggaaaggc aggcggcgat gatcgcgccg
     2521 gtgctggtac cggcaaaaaa atcaaagtaa tccgcgagca cgaaatcgtt gcctctgcct
     2581 agcttacgcc gcagcatact ttcaattttt gccagcactt ccacggttaa aatgccgcgg
     2641 atgccgccgc cgtccagtgc tagtattttt ttcggtccgg tgctgttgat cttttccgcc
     2701 acgctcattg ccgacctcct tcaccacaaa gtcgttgtgt cgatcacata aaccctgccg
     2761 gaataccttt gcacggtctc aaacatatgc tgggttccgc cgggaccatc gccgcctttg
     2821 cggttccata aacagacaag ccgcaccttc tctggccccc atgccagggc tgtgtagagc
     2881 tgccaaagat taaggcgcgc gaaagggttt gctgccgcag gcgaaggccc tagcatttcg
     2941 ggcatgaccc acgttgtagt atttggatga gatttgactt tgaagtagcg ctcggtccag
     3001 ttgccaggag cgaaggagac cgacttctgc aaaaatgtcg gctcgtcaaa ggctaaccgc
     3061 acttcaagtt tgagcccacg ctccagcgca gcttccgcaa acaacaggtc gccgccgcat
     3121 gcgccgccgc agagagccag atcctccgcc ccggcgtcca gcttctccag ggtagccgct
     3181 atcgctgctg cggcgatatt ctccttatca gcaggaaatc gcggctcggt ccggtctgga
     3241 gcatcgatca tatgaccact gaacagcaaa acctgtcgtg gcgtccaggt cttatctggg
     3301 ggattgagcc ggcttaaggc gcgatcgaga accgccactg cggcttcaac ttcctccgga
     3361 cgaaaaccca ggagtttcag cagctcgagt tgctggcgcg acgaattgag tttgaaccaa
     3421 tctttatcag ccaccgccac ggcatttttg tatgcggcat ccaccgcctg cttgttgcca
     3481 gccaaaactt ccaattcggc taaactgatg cgcgcccagt aatccttggg atttctctcg
     3541 agggcgccgc ggattgccca ccgaactccg ccctccattt ccgtgcgcac atcggctttc
     3601 acctcatcgg ctgacaggtg agccaataga tgcagcaagg tcacagcgtt aattcctgag
     3661 tagtagtgcg ccggatcttt gcggaacccg gtggcgtatg cgttgatggc ttcgcgcagg
     3721 gttgcgcttt ccatctcggc ttcccggcgc atatcaaccg cagatcggtt gtcctgtctc
     3781 cagcaattca cccaagcgtc tttttcgacg cgcccgcgca ttgaccaact ctcggcatct
     3841 tcagggaatt caccgagtag atcatcgatg cactcttggg catcgtcatg cttcttta
//
LOCUS       DASUTG010000622         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_540587, whole genome shotgun sequence.
ACCESSION   DASUTG010000622 DASUTG010000000
VERSION     DASUTG010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540587"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VF089_14355"
     CDS             <1..1693
                     /locus_tag="VF089_14355"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175191.1"
                     /translation="TMFDLILKGGRVYDGSGMPSFTGDVAVAGGKIAAIGRLSAPAKR
                     TMNVEGLAVAPGFIDPHTHLDAQLLWDPLGTSSCFHGVTSSVVGNCGLSLAPCKREGR
                     EAVIKSFVRVEAISRKVLEEGVEWKWVSTGEYLDALGSRLGINVAALIGHIAVRHYVM
                     GEDAVEREARPEEIAQMQQLVREGMAGGAVGFSTNQNPRHMREDGKPVASRVASHQEL
                     LALVEVLGDMNTGVVQLSGGGVDARGRIAYASELARATGRPVLWQSISHSWTRPDHWK
                     GMLESTARVFRDEGLPIYAMTQAKPFENRYTLLDAQCFDEFPTWRAAMFMPVEQRQKI
                     FADPEARQKMRAEVNSPEPSVFPKRWDVIYVDEAKLPKNKSLQGRTIEEIARSQGKDP
                     MDVFLDMALEENLETRFSHVTTQGDPKAVCEILKNPFVIIGQSDAGAHMGYDARFGYC
                     TTFLGRWVRDNRIMSLEEAVSKLTFHVASIFGLRDRGLLRSGLAADIAVFDPETVNTV
                     PPEYVQDLPGNERRMIQRATGVHHTVVNGEVVIENGAVTGAYPGRVLRNTPPPRD"
     gene            1807..2361
                     /locus_tag="VF089_14360"
     CDS             1807..2361
                     /locus_tag="VF089_14360"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6175192.1"
                     /translation="MGILNTFLNDRVALRVGDITKEQVDAIVNAANGTLMGGGGVDGA
                     IHRAGGPDILKQCKEIRAKEFPHGLPTGEAVITTAGRMAAKHVIHTVGPVYGRGGDEK
                     AQLLASCYRNCLRLAVEHGLKTIAFPAVSTGIYGYPLGEAAKVSSQTIEEFFGSNASL
                     EQVRLVFFSQNDADAFLKNQAFTH"
     gene            2485..>3746
                     /locus_tag="VF089_14365"
     CDS             2485..>3746
                     /locus_tag="VF089_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6175193.1"
                     /translation="MTSFQRRPVENVLSAKSVAIVGASPKGRWPMGIFRNLKKAYQGK
                     IFLINPNYQEIENHPCYPNLAALPEVPEELLILIPTRAVLGTLEEATNLGTKSATIYT
                     AGFGEGDDPKGKERAQAMKDLCERTGFVLCGPNCMGSYSLPEGLWTFPTSTPLLKKGP
                     VGLIFQSGGSLGNWIKGATERGIGFTYAVSSGNEISLDLVDYLSFMVDDPETKVILLM
                     AEGIRRPTEFMAVAREALNKNKPILVVKLGRSEMGKRQAISHTGALAGADEVFDAVSN
                     RLGLIRCPTLEDLTEMALAFMAGRYPRGSRAAIVVNSGGMKGLICDHAEELNTNLAQL
                     SDKTKEAVRPLIPPELAIENPLECGVAGFGDEQGFINIVKLHAEDDGVDLLAIHGELP
                     RFPEKREATLFKNLAAATDKPILAFARST"
BASE COUNT          821 a          960 c         1126 g          839 t
ORIGIN      
        1 cacgatgttc gatcttatac tcaagggtgg aagagtttat gacggttcgg gaatgccgag
       61 cttcaccggc gatgtcgctg tggcaggagg aaagattgct gcaatcggcc gtctcagtgc
      121 gccggccaaa cgtacgatga atgtcgaagg cctggcggtg gcgcctggat tcattgaccc
      181 gcatacgcat ctcgatgcgc agttgctctg ggatccttta ggcacgtcat cgtgttttca
      241 tggcgtcacc tcgtcggtgg tgggaaattg cgggctgagc ttggcgccat gcaagcgtga
      301 ggggcgcgag gcggtgatca aaagctttgt tcgggttgaa gccattagcc gtaaggttct
      361 cgaagaggga gtcgaatgga agtgggtatc caccggcgaa tacctggatg cgcttggctc
      421 gcgtctgggc atcaatgtcg ccgcgctgat cggtcatatt gccgtgcgac attacgtcat
      481 gggagaagac gccgttgagc gtgaagcgag accggaggaa atcgcccaga tgcagcagtt
      541 ggtgcgcgag ggcatggccg gcggcgccgt gggcttttcg accaatcaga acccgcgcca
      601 tatgcgcgaa gacggcaagc ctgtagcaag ccgggtagcc tcccatcaag agctcttggc
      661 cttggtagaa gttctcgggg atatgaatac gggcgtggtt cagttgagcg gtggtggcgt
      721 ggatgcgcgg ggacggattg cctatgcatc cgagctcgcg cgcgccaccg gccggccggt
      781 attgtggcag agcatcagcc atagttggac ccggccggat cattggaaag gcatgcttga
      841 atccaccgcc agagtttttc gtgatgaggg attgccgatc tacgcgatga ctcaggccaa
      901 acccttcgag aaccgctata ccttgctcga tgcgcagtgt ttcgatgagt ttcctacctg
      961 gcgcgccgcc atgtttatgc ccgtggagca gagacagaag atttttgctg accccgaagc
     1021 gcggcagaaa atgcgtgccg aggtaaactc gccggagcca tcggttttcc ctaagcgttg
     1081 ggacgtcatt tatgtcgatg aagcgaagct gccgaagaat aaatctctgc agggtcgaac
     1141 tatcgaggag attgcgcgaa gccagggaaa agaccccatg gatgtgttct tggatatggc
     1201 attggaagaa aatctcgaaa ctcgcttctc acacgttacg acgcaaggag atcccaaagc
     1261 ggtttgtgag atcctcaaga atccgtttgt catcatcggt caatccgatg ccggcgcgca
     1321 catgggctat gacgccaggt tcggctactg cacgaccttt ttgggtcggt gggtgcgcga
     1381 caatcggatt atgagcttgg aggaagcggt ttcgaaacta accttccacg tggcatcgat
     1441 attcggcctg cgcgatcgcg gcttgttgag gtccggactt gcggctgata tcgccgtgtt
     1501 cgatcccgaa acggtcaata ccgtgccgcc cgagtatgtt caagatctgc ccggcaacga
     1561 gaggcgaatg atccagcgcg caaccggcgt gcatcacacg gtagtcaacg gggaagtggt
     1621 catcgagaac ggcgccgtga caggcgccta tccgggacga gtattgcgca acacgccgcc
     1681 gccacgggat tgatgttcgt tcgctggtca gacgaccggc agcgggtaaa gcgctctgtc
     1741 gtgagtctcg ctccggggat cttttattta gctgctcgcc gagggagccg gatagccgac
     1801 aaaacgatgg ggattttaaa cacttttctc aacgatcgag ttgcgcttag agtcggcgac
     1861 atcaccaaag aacaggtcga tgcgatcgtc aatgccgcca atggcacttt gatgggcggt
     1921 ggcggcgtcg atggcgcgat ccatcgcgca ggtggcccgg acatattgaa gcaatgtaaa
     1981 gaaatccggg ccaaagaatt tccccacggg ctgccgaccg gagaggctgt cattaccacg
     2041 gccggcagaa tggctgctaa gcacgttatt cataccgtag ggccggtcta cggaagagga
     2101 ggtgatgaga aggcacaatt gctggcttcc tgctaccgaa attgtcttcg cctggcggtt
     2161 gagcatgggc tcaagacaat cgccttcccc gccgtatcca caggtatcta cggttatccg
     2221 ctcggcgaag cagccaaggt gtcatcccaa acgattgaag aatttttcgg atcaaatgct
     2281 tctctcgaac aagttcggct agtctttttt agccagaatg atgccgacgc gtttttgaaa
     2341 aaccaggctt ttacacacta gccgcccgat cacagtgctt tccttcctga tgcgcgtcga
     2401 cagcgcgcta tcatctcacc tgtgttgcag cgacgtttag aacatgagat aggaaaattt
     2461 gattcaagtt gataaggaac tcgtatgact tcattccagc gcagaccggt cgagaatgtt
     2521 ctttctgcca aatcagtagc cattgtcgga gcgtccccga aagggcgttg gccgatgggg
     2581 atttttcgca atttaaagaa ggcttaccag gggaaaatct tcttgatcaa tccgaactat
     2641 caggagattg aaaatcatcc ttgctatcct aatctcgcgg cgctgccgga ggttcccgag
     2701 gaactgctga ttttaattcc gacccgagcg gtgttgggca ctttggaaga agctactaac
     2761 ctcggtacca agtcggccac gatctacact gctggttttg gtgaaggcga cgatcccaag
     2821 ggaaaggaac gtgcccaagc gatgaaggat ttatgtgagc gcacgggttt tgtcctttgc
     2881 gggccgaact gcatgggctc gtactcttta cccgaaggcc tctggacgtt tcctacttca
     2941 acgccattgc taaagaaagg tccggtgggt ctgatctttc agagcggcgg ctcactgggt
     3001 aattggatta aaggcgcgac cgagcgcggc attggtttta cctatgcggt ttccagcggc
     3061 aatgaaatca gcctcgacct tgtggactat ctctcgttta tggtggacga tccggagacc
     3121 aaagttatcc tgctgatggc ggagggcatc cgccgcccca ccgaattcat ggctgttgcg
     3181 cgtgaggctc tcaacaagaa caagccgatt cttgtcgtca agcttgggcg ctcggaaatg
     3241 ggcaagcggc aggcgatctc gcacacggga gcattggccg gcgccgatga ggtgttcgat
     3301 gcggtgtcca accggcttgg tctgatacgg tgcccgactt tggaagatct aacggagatg
     3361 gctttggcct tcatggccgg ccgttatccg cgcggcagtc gcgccgcgat cgtggtcaac
     3421 tcaggtggca tgaaaggcct gatctgcgat cacgccgaag aactgaacac caatctagcg
     3481 caattgagtg acaagaccaa agaggcggtg cgcccgctga tcccgccgga gctggctatc
     3541 gaaaatccgc tcgaatgcgg cgttgccggt tttggcgatg aacagggctt catcaatatc
     3601 gttaagctgc atgccgagga tgacggggtc gatttacttg cgattcacgg cgaattgccg
     3661 cgcttccctg agaaaaggga agcgacgttg ttcaagaatc ttgccgccgc aactgataaa
     3721 ccgattcttg cttttgcccg ctctac
//
LOCUS       DASUTG010000623         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_690370, whole genome shotgun sequence.
ACCESSION   DASUTG010000623 DASUTG010000000
VERSION     DASUTG010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690370"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VF089_14370"
     CDS             <1..676
                     /locus_tag="VF089_14370"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEX6175194.1"
                     /translation="CFMGDFQRAERQLDAVAQVSGDATVELGIEIYRGILRAEASRRQ
                     LFQGQSRQPKFLSEPPPYTALHLKALTEIIEQHFDEAETSLKESSDLRKSLQGQIDDT
                     HFDEFKDGDDLLAPFLEVIIRGDYLWLALEDIVRLEIRRPRTLRDLLWIPGKLDLREQ
                     QTIDVFIPVQYYGSSDHPEDPVKLGRMTVWENVGEETNLGKGQRTFLIDGDERSLLEI
                     RKVEFA"
     gene            779..1987
                     /gene="icmH"
                     /locus_tag="VF089_14375"
     CDS             779..1987
                     /gene="icmH"
                     /locus_tag="VF089_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX6175195.1"
                     /translation="MGEQQSSTWIHKGIASITRIGRANVPPVATTGGTNRLREIFTDL
                     ISFVIFFKSSCDKQAPDQNELREKILALATAQEERVKGAGISADNFREARFAVFSWVD
                     EMILTSTWPHRTRWQHLMLSYYKTFNAGEEFFVRLQNLPPHANDVREIYYLCLSLGFL
                     GQYAFGDGPSEVNKLKQALYMQLSGRQGDTRRNYGLLFPEAYQKAVTAARAPDRAKFF
                     WYVVALSTPVVLFITYFVLLRYQADQLIAKISRVEQAPLTPKAIKKPWFGSLVEELRR
                     KGVRAVEQPEGVLITLDTLVFTAGSAQLNAQARAQIDDIVTTVKRYAPDKAVRIEGHA
                     SREREADEPRNRKLSEDRARTVAEAFTRSGFRSTQIFAQGLGSSKPVASNDTEQGRSQ
                     NRRVEITVRK"
     gene            1984..2895
                     /locus_tag="VF089_14380"
     CDS             1984..2895
                     /locus_tag="VF089_14380"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX6175196.1"
                     /translation="MSNSSLAVVVDSYGLSDVGLVRTNNEDSFLIATPTRDFANENPA
                     ELSGIIPQNGTMFIVADGMGGAQAGEVASRMAVETVGRNFINALEKQAVLDQQSLITA
                     LTETIREANELIFQQGQKNTELNGMGTTLTAATVFGSTILFAQLGDSRAYLARKGVIS
                     QMTKDQSLVAQMVASGSLTPEEAKTHPQRNVILQALGIQNQVEVAITLAELRRGDQIV
                     LCSDGLSGKVDVEEIREFLEKFEPKAACQGLVRMARERGGEDNITVIVARFNGDGLSE
                     PEPEDVTGPPNTKMEPARKRSFWPWRR"
     gene            complement(3267..3343)
                     /locus_tag="VF089_14385"
     tRNA            complement(3267..3343)
                     /locus_tag="VF089_14385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3307..3309),aa:Ile,seq:cat)
     gene            complement(3384..>4449)
                     /gene="rpoD"
                     /locus_tag="VF089_14390"
     CDS             complement(3384..>4449)
                     /gene="rpoD"
                     /locus_tag="VF089_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6175197.1"
                     /translation="LQLNRKQTEAIVDGLKRAMDRMQTLERRVKEFEQRTGKSASELL
                     KLVANLNGRKKDWQRLASSLRMGHDAVLHMSEEIRNARRDIRHIEKDLDIPAEENKRR
                     VWSIVDGETKANLAKKELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKF
                     EYQRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQEIGRE
                     PTPEEIATKMEIPLDKVRKVLKIAKEPISLETPVGEEEDSHLGDFIEDKRIITPSDAV
                     VNINLQEQTKKVLATLTPREEQVLRMRFGIGEKSDHTLEEVGQKFTVTRERIRQIEAK
                     ALRKLRHPSRSKRLKSFMET"
BASE COUNT         1093 a         1102 c         1161 g         1093 t
ORIGIN      
        1 gtgtttcatg ggtgatttcc agcgagctga acgccagctc gatgctgtgg ctcaggttag
       61 tggcgatgct acggtcgaac ttggcataga aatttatcgg ggtattctcc gggcggaagc
      121 cagccgtcgt caactatttc aaggacagag ccgccaacca aagtttttgt ctgaaccgcc
      181 accctatacg gcgttacatc tgaaagcgct tacggagatc attgagcagc atttcgatga
      241 agcggaaaca tcgctgaaag agagcagcga tctcaggaag agcttacagg gtcaaattga
      301 tgatacgcat ttcgatgaat tcaaagacgg tgacgatctc ttagctcctt ttttggaggt
      361 cattatccga ggtgactatc tgtggctggc gctcgaagat attgtcaggc tcgagattcg
      421 gcggccgcga actttaagag atcttctttg gattcccggc aagctcgatt tgcgtgagca
      481 gcaaacaatt gatgttttca tacctgtaca gtattacggt tccagtgatc accctgaaga
      541 tcccgttaaa ctcggacgaa tgacggtttg ggagaatgtc ggggaggaaa cgaacctcgg
      601 taaaggtcag cgaacctttc tgattgatgg agacgaacgc tcactactgg aaatcagaaa
      661 ggttgaattc gcctgaccgt aagtatacct gccaatcgac taacccgaca caagaagatt
      721 caatatttct aacaggaact ccgtttgaga agagcttggt atggctaact gaagttacat
      781 gggagagcaa caatcctcaa cctggattca taagggaatt gccagtatta ctcgaattgg
      841 ccgcgccaat gtgccgccgg ttgcaacgac gggaggcacc aatcggctac gcgagatctt
      901 taccgatctc atttctttcg taatcttttt caagtcctcc tgcgacaaac aagcgccgga
      961 tcaaaacgag ctgcgcgaga agattcttgc gctcgcgacc gcccaagagg agcgagtcaa
     1021 aggagcgggg atttccgcag acaatttccg cgaagcacgt ttcgccgttt tttcctgggt
     1081 agatgaaatg attcttactt cgacatggcc ccaccgcacc cgttggcaac atctaatgtt
     1141 aagttattac aaaactttta acgccgggga agaattcttc gtccgtttac agaatcttcc
     1201 gccgcatgcc aatgacgtgc gcgaaattta ctatctgtgt ttgagcctcg gcttcctcgg
     1261 acaatatgct tttggtgacg gcccgtctga agttaacaaa ctaaagcaag cgctctacat
     1321 gcaactttct ggaaggcaag gtgacacgcg tcggaactat ggcttgttat ttccagaggc
     1381 atatcagaaa gctgttactg cagcgcgggc acctgatcgc gcgaaattct tttggtatgt
     1441 cgtcgcccta agcacgccgg tcgtattgtt catcacgtat ttcgttctcc tcaggtatca
     1501 agccgatcag ctgatcgcca aaatctccag agtcgaacag gcgcccctca cacctaaagc
     1561 aatcaaaaag ccttggttcg gttcgctagt tgaagagctg agacgtaaag gtgtgcgtgc
     1621 tgttgagcaa ccggaaggtg ttctgattac gctcgataca ctagttttta cggccggcag
     1681 cgcgcagctg aacgcccagg ctcgggcaca gatcgatgat attgtcacga cggtgaagcg
     1741 ctacgcaccg gacaaagccg ttagaataga gggccatgcc agcagggaaa gagaggcaga
     1801 cgaaccccgt aatcgtaaat tatctgaaga tcgcgcgcgc actgtggcgg aggcatttac
     1861 acgctctggt tttcgcagta ctcaaatctt cgctcaaggg cttggcagtt caaaacccgt
     1921 ggctagcaat gatactgaac aaggccgcag tcaaaatcgc cgcgtggaga tcaccgtaag
     1981 aaaatgagca actcaagctt agccgtggta gtagacagct atggattgag cgatgtcggc
     2041 ttggtccgta caaacaacga agacagcttt ttgatcgcaa ctccgaccag ggactttgcc
     2101 aacgagaatc ctgctgagtt aagcggcatc atcccccaga atggaaccat gtttattgtt
     2161 gcagatggca tgggtggagc tcaagcagga gaagtggcaa gccggatggc cgtcgaaacg
     2221 gttggacgga actttatcaa tgctctcgaa aaacaagccg tcctggacca gcaatcgttg
     2281 attaccgcgc taaccgaaac tattcgagag gcaaacgaac ttatctttca gcaaggacag
     2341 aaaaacactg agctcaacgg catgggcaca acgctcaccg ccgccacagt gttcggcagc
     2401 accattttat ttgcacagct tggagacagc cgggcctact tggcgcgtaa aggggttatc
     2461 tctcaaatga ccaaagatca aagccttgta gctcagatgg tcgcctctgg ttcgttaacg
     2521 ccggaggaag ctaaaaccca tccacaacgg aacgttattc tccaggcttt gggaatacag
     2581 aaccaggttg aagtagcgat tacgctggca gaactcagga ggggcgacca gattgtactg
     2641 tgcagtgatg gtctatcagg aaaggttgat gtcgaggaga tcagagagtt tctagaaaaa
     2701 tttgagccga aagcggcctg tcagggactc gtgagaatgg cccgagaaag gggtggcgag
     2761 gacaacatta ccgtgattgt tgcgcgtttt aacggcgatg gactttcgga accggagcct
     2821 gaagatgtta caggtcctcc taacacaaag atggaacctg cgcgcaaacg ttccttttgg
     2881 ccctggcgga gataaatgga cttatcggta tctaggtagg caccttgaac agttgtgctt
     2941 ctggcttccg agcaactcag acttgccttt cggatgttac ccgcttgagt tcggcgcctc
     3001 tcgattgaac gcgccgctgg acgaataaag aacttcacgc agactcaaga agaaacaaac
     3061 gtgcgatccc ggtgcgtagt gagaccgtct cagccatcga tcgcacatgc atgcttacgt
     3121 aatgggatgg ttcgacgtag tgcgaagaag attgcggcaa cacgggaatc tgcaaatggt
     3181 ctcaagcacg tgagaagcga acggcaaaac acagccgcac cactcttccc catagaacgt
     3241 ccgagaagtt ctttacggag gtgaattggt gggcccacct ggactcgaac cagggaccaa
     3301 ccggttatga gccggtggct ctaaccaact gagctatggg cccgtaacaa aaaaatgtgc
     3361 ttgagatggt aggaaactga tgctcaggtc tccatgaagc tcttcagcct cttgctgcga
     3421 ctgggatgtc ttagcttccg cagcgccttg gcttcgattt gccggatgcg ctcgcgtgtg
     3481 acggtaaact tttgaccgac ttcttcaagg gtgtgatcgg atttttcacc gatcccgaac
     3541 cgcatccgca gaacctgttc ttcacgaggg gttaaagtgg cgagtacctt ctttgtttgt
     3601 tcttgcaggt tgatattcac caccgcgtcg gaaggagtga taatcctttt gtcctcgatg
     3661 aagtccccga gatgactgtc ttcttcctcg cctaccggcg tctccaggga tatgggctct
     3721 ttggcaattt tgagaacctt ccttactttg tctaagggaa tttccatttt cgtggcaatt
     3781 tcctcaggag tcggctcgcg gccaatttct tgcactagat accgcgatgt ccggataagc
     3841 ttgttgatgg tttcaatcat atgaaccggt atgcgaatgg tccgcgcttg atcggcaatg
     3901 gctctggtga ttgcttgccg gatccaccag gtggcatagg tagaaaactt gtatcctcgc
     3961 tgatattcga acttatcgac ggctttcatc aagccgatat ttccctcttg gatgaggtcg
     4021 agaaactgca gcccgcgatt ggtgtacttc ttggcaatac tcacgaccag tctaagattt
     4081 gcttcgatca gttctttttt cgccagatta gctttggtct caccatcaac gatcgaccaa
     4141 acacgacgct tgttttcctc ggccgggatg tcgaggtctt tctcgatatg ccgtatgtcg
     4201 cgccgcgcgt tcctgatctc ttctgacatg tgcaggaccg catcgtgccc cattcgtagc
     4261 gaactggcga gccgttgcca atccttctta cgtccgttga ggtttgcgac aagcttcagg
     4321 agttcggatg cgctcttacc ggtcctttgc tcgaattcct tcacccgacg ctctagcgtt
     4381 tgcattcgat ccatggctct tttgagacca tcaacaatcg cttcggtttg ctttcgattc
     4441 agttggagg
//
LOCUS       DASUTG010000624         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1577816, whole genome shotgun sequence.
ACCESSION   DASUTG010000624 DASUTG010000000
VERSION     DASUTG010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577816"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VF089_14395"
     CDS             complement(<1..743)
                     /locus_tag="VF089_14395"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX6175198.1"
                     /translation="MYPILFQLGPITIYSFGAFMALAALSAAWVVHSELKRRGYDPEV
                     ASSMVFAAAIGGLIGARLLFILGDWNNFLASPSSYIFTGAGFTWYGGLFGGAVAVSWI
                     VRKNQIPWLVGADIAAPALAVAYGVGRLGCHFAGDGDWGSITDVPWGVAYTNAIIGWV
                     DPMTGVPYPPGTRVHPTPIYEFLQSISIFAILWAMRSKGLAPGTIAWLYLVLAGLGRF
                     IIEFWRVNPMLAGGLSEAQWISLILMAVG"
     gene            complement(789..1532)
                     /locus_tag="VF089_14400"
     CDS             complement(789..1532)
                     /locus_tag="VF089_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175199.1"
                     /translation="MKQVKVPRLLILVLIALNGGIATAGSSDFHVHPAPLISSDGQLR
                     GAVLFSGKYQRPAPLKVVKSRSFCGLTVPNETLLVDPGGGVRNAVVILRPLGRAVNVK
                     PGSILLDNKRCAFTPHVQIAGIGSELLLKNSDPILHTVHARLGNETLFNVGLPHWRQV
                     SKVLDRLGVIRIDCDVLHTWMSAAIVVVSTPYAAVTGEDGSFVMDEIPAGKYDVEIWH
                     EVLGTKKAQISVPQSGTISLEAVYGQIPG"
     gene            1734..2171
                     /gene="dtd"
                     /locus_tag="VF089_14405"
     CDS             1734..2171
                     /gene="dtd"
                     /locus_tag="VF089_14405"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6175200.1"
                     /translation="MRAVIQKVTKARVTVGENIVGEISSGLCILLGVGREDVAADADF
                     LVGKIKNLRIFEDQEGKMNLSLLAVGAEVLVVSQFTLYANWRKGNRPSFTDAAPLPHA
                     EKLYNHFIERLRNAGVSVATGQFQARMKVSLVNDGPVTFVFES"
     gene            2168..2626
                     /gene="trmL"
                     /locus_tag="VF089_14410"
     CDS             2168..2626
                     /gene="trmL"
                     /locus_tag="VF089_14410"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX6175201.1"
                     /translation="MSMHVVLFQPEIPPNTGSIARLCAATLTPLHLIEPLGFKLDDRH
                     LKRAGLDYWQFVDLRVHKSWDEFLAAAPARQLLYFSKRAVRSYTTARYQQNQYLVFGP
                     ETRGLPRHLLDANSDHSYRIPMVGEGVRSLNLSNAVSIVLYEGLRQLGKV"
     gene            2801..>3045
                     /locus_tag="VF089_14415"
     CDS             2801..>3045
                     /locus_tag="VF089_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175202.1"
                     /translation="MAAESSEQKDAAPSKESFNLIIPYKDLTVGQGQEVTMDTEVVNR
                     TKNPVEVNLSLESIPKGWEANFNSRYPSYPVRSVTVQG"
BASE COUNT          751 a          818 c          792 g          684 t
ORIGIN      
        1 aatccaacgg ccattagaat aagactgatc cactgggcct cgcttagacc gcctgccaac
       61 atcggattga ctcgccaaaa ctcgatgata aatcgcccaa gccccgccag aacaaggtag
      121 agccatgcga ttgttccagg ggcaagtccc ttgcttctca tagcccagag gattgcgaag
      181 atagagattg attgtagaaa ctcgtaaatg ggcgttggat gcacgcgggt acctggtgga
      241 taaggaacgc ccgtcatggg atcaacccaa ccaataatcg cgttagtgta tgcaacgccc
      301 caaggcacgt cggtgatcga accccagtcg ccgtctccgg cgaagtggca acctaggcgt
      361 cccacgccat aggcaacagc cagggcggga gcggcaatat cagccccgac gagccatgga
      421 atttgatttt tcctgacgat ccaagatacc gccaccgcgc cgccgaacaa tcctccatac
      481 caggtaaatc cggcaccggt aaaaatgtag ctcgacggag aagctaagaa gttattccaa
      541 tcacccagga tgaacaaaag ccgggcgccg atgagtccgc cgatagcggc ggcgaagacc
      601 atcgaagacg ccacttcggg atcatagcct cgccttttta gctctgagtg aaccacccaa
      661 gcggccgata gcgccgccaa agccatgaaa gcgccgaagc tgtaaatcgt tatcgggccg
      721 agctggaaga gaattgggta catggaaacc gatcataccg gagagacggc cagtagcaaa
      781 ccacgatttc atccggggat ctggccatag acagcctcga gcgaaatcgt gccgctttgc
      841 gggactgaga tttgcgcctt cttagtgccg aggacctcat gccagatctc gacgtcatac
      901 tttcctgctg gaatctcatc catcacaaat gacccatctt caccggtcac ggcggcatac
      961 ggggtggaaa ccaccacgat agctgcgctc atccaggtat gaagcacatc gcaatcgatt
     1021 ctgatcacgc ctaacctgtc gagaactttg gaaacctgcc gccaatgggg caaacccaca
     1081 ttaaaaagcg tctcgttccc gagccgcgca tgaactgtat ggaggatcgg atcactgttc
     1141 ttgaggagca actcgcttcc gatgccggcg atttgcacgt ggggtgtaaa cgcacatcgt
     1201 ttgttatcca ataagatact gccaggtttg acgttcacag ctcgaccgag cgggcgcaga
     1261 ataactactg cgttccggac cccgcccccc ggatccacaa gcaatgtttc gttaggcacc
     1321 gttaaaccac agaagctgcg gctctttacc actttgagcg gagctggccg ctggtatttg
     1381 cctgaaaaca aaacggctcc tcggagttgc ccgtcgctgg aaatcagcgg cgccgggtgg
     1441 acgtgaaaat ccgacgatcc ggcagtggca atgccgccat tcaatgcaat tagaaccagt
     1501 atgagcaatc gcggaacctt gacctgtttc atcttgtctt gcggactgtg caaacctcgc
     1561 actgtcgggg cttgcgagcg aatcaaaccc gcttcgcctg ctcgttagaa ctcacaaacg
     1621 gctgttgaga ctctactatt tctctgttac gactgctcgc agtatcttgt gattccactc
     1681 ttgactagcc cgcctcacta gtcgcacaag ctttttgggt taataaggga atcgtgcgcg
     1741 ccgtcattca aaaggttaca aaagcccggg tcacggtcgg cgaaaacatc gtcggcgaga
     1801 taagttcggg cctgtgcatt ttactaggtg taggccgaga agatgtcgcg gcggacgccg
     1861 atttccttgt aggaaagatc aagaatcttc gcatcttcga agaccaagaa gggaagatga
     1921 atctttcttt attggctgtc ggggcagagg ttttggttgt ctcgcaattc actctttatg
     1981 ccaactggag aaaaggcaat aggccatctt tcacagacgc ggcgccattg cctcacgccg
     2041 agaaactcta caaccacttc atagagcggc tccggaatgc cggagtttca gttgcgaccg
     2101 gccagttcca agccaggatg aaggtttcgc tggtcaatga cggtccggtt actttcgttt
     2161 tcgaaagttg agcatgcacg tagttttatt tcaaccggag attccgccga acaccggtag
     2221 catagcccgg ctatgcgcag ctacgctgac ccctcttcat ttgatagaac cactgggatt
     2281 taaactcgac gaccggcatc tcaagcgcgc cggacttgac tactggcagt tcgtcgatct
     2341 gcgggtacac aaatcctggg atgagttcct ggctgcagcg ccggcgagac agttgctcta
     2401 cttttccaaa agagcggtga gatcctatac cacagcacgg taccagcaaa accagtatct
     2461 agtcttcggg cctgagacgc ggggcttacc gcgacatttg ttggacgcca attcagatca
     2521 ttcctataga ataccaatgg ttggtgaagg tgttaggagt ttgaatctct ccaacgccgt
     2581 gtcgattgtt ctctacgaag gattacggca gctcggaaaa gtttaacttt acgccgcaag
     2641 ctcttgaccc catgaattaa tctagttatt tattgcacca aatttcggat cttagggggt
     2701 ttttgatatg aggtttgtgc gtctacgtgt gcgccggcga atgctacctg tgctgttctc
     2761 gtccttttta ctttgttcaa cttcgctcgg agcgtcagca ttggccgccg aaagcagtga
     2821 acagaaggac gctgcgccct ctaaagagag tttcaatctg atcatcccgt acaaagactt
     2881 gacggtcgga cagggacagg aagtcacaat ggatacggag gttgtgaatc gcacgaaaaa
     2941 tccggtcgag gtgaatctgt cgctggaatc gattcccaag ggatgggaag cgaacttcaa
     3001 ttccagatat cccagttatc ccgttcgctc agttacggtt caggg
//
LOCUS       DASUTG010000625         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_245289, whole genome shotgun sequence.
ACCESSION   DASUTG010000625 DASUTG010000000
VERSION     DASUTG010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245289"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..2781
                     /locus_tag="VF089_14420"
     CDS             376..2781
                     /locus_tag="VF089_14420"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6175203.1"
                     /translation="MTVESNPLESGALVGLRVLDFTSRMGGYCGLLLANLGAEVLLIE
                     PPGGDAMRREGPFKEDSLNPEGSISFAAYHTNKRGIVLDLESDGDRASLRRLLGKADV
                     LIEDRPVGFWQRLGLGYQDLQAINPALVMTSISGFGGAGPYRDFKAPNIVAFAMGGLM
                     NLCGHPERAPLSGPCDLAYRLGSVHAAFGTLVALLNRRASGRGDHLDISLQDVLVADP
                     FLRIITRYSVTGEIPQRTGHSQATTVAETYQCKDGFARIFCNQPDHWRRLVEWLGNPP
                     ELIDPKFENVQNRLPLRPLLDRLIEARTLNYDVKSFFDEFQSRRLAAAPINSPSAFLE
                     DEQTRDRDYVVEVDHAHFGRHRFPGDPYKFSESPWHITRGAPLVGEHQSMITSGWSQP
                     SPALPSLKPVGSDSRAFAGIRVISFPTGIVGPALGCLLAEHGAEVISIEAGRTVRSPQ
                     RGQRFQIVSDLESNRGRKRIAINMKHSEGVDIAKRLIARSDVVAENFSARVMASWSLD
                     YPRLREVRSDIIMASLQAFGQTGPRRDYVSFGPILMAYSGMAYLWRDPEIERPGAACQ
                     TAFPDYIAPSYGALAILAALHYRARTGKGQYIDLSQAETAASLIAPAHLEWLLTGREP
                     QPSGNFSASAAPHGCYKCKGEDRWCVISVQTEEEWAQFCDLVGHREWLADARFTDLLS
                     RVANGPELDRLVESWTARHTPHQVMMILQRNGVAAGVVQTGEDLYRDPHLRERGSTRE
                     VFHQPIGWVTRAGPSVRLSEAKFTGNNQAHFAGEDNEAVFGELLGMSSQQIADLRQRE
                     VLR"
     gene            complement(2819..3037)
                     /locus_tag="VF089_14425"
     CDS             complement(2819..3037)
                     /locus_tag="VF089_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175204.1"
                     /translation="MKIVLLALAMLTAACASPPPPAQTRDKTTTTASPSSSSRDTTDC
                     ERQAALAGAGERAKAFENCMRSKKTPQR"
     gene            3537..4598
                     /locus_tag="VF089_14430"
     CDS             3537..4598
                     /locus_tag="VF089_14430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175205.1"
                     /translation="MGAIDADAHVIETVQTWSYLENDERRFTPQLMTQTFGEKLLSNE
                     GIAVPEFWVIENRAFGKDRNVGTDTSKESREMLSVQARLAHMDELGIETQVLYPTLFL
                     RPVLQDAERERALCKSYNRWMGELWRQGQGRLRWVAKPPLRLLERMPAAIKQELEWSK
                     ENGACGIFMRGLECERALGAPYFYPLWEMAAELDLPVCIHSANGSFIHHEFFADDTSF
                     TKFKLTVVGAFHTLLEKEIPNKFQKVRWGFVEVSAQWVPYVLNDLADRFRRQGRPFPA
                     DPLGANNLWVACENTDDLAYVLRHAGEDCLMIGTDYGHHDPSTELNAIHLLRNDKRIA
                     PNVVSKILETNPKKFYGLN"
     gene            complement(4724..>5327)
                     /locus_tag="VF089_14435"
     CDS             complement(4724..>5327)
                     /locus_tag="VF089_14435"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175206.1"
                     /translation="RVHAIGDKAFDMALDAYENAIEHSPRKDHRHRIEHMGNWLATPK
                     LMQRMVRSGIVAIPNIAFAYYIGDAILDCVGERRLTKAFPFRTLLKNGIIIAGGSDSP
                     GYWPVDPLRDIATAASRKMRWGDVWVPEERISVSEAFAMHTTTASWVGFEENDKGTLE
                     VGKLADIAVLAEDPFEVQPERIKDLKVEMTLVGGEVKYQA"
BASE COUNT         1170 a         1478 c         1476 g         1203 t
ORIGIN      
        1 tcagcccagg ccatttcatc atcaataatc cgcctaaaag cacgaacacg acaaaggcta
       61 gatggatgca gacaaccaaa tcggcaagca atggataaac tgtttgcgct gtaatcattt
      121 tcaaaatgga atcactacga aagccactcg catgacagta agcgtggggc gtctcttgtc
      181 atttatagtc cgctacaaat atattcccaa acgggtccgg tcatgtgtgc aaccgttgta
      241 ggagggtctg aagcccacct tttgcgagtg aattccagct tgtcggattt ccctcgcaca
      301 tcgacaggat gacatcagag tttctgacct gtgatttcga aacttttatg ttgagacttc
      361 tcaaggatct atcgaatgac tgttgaatcg aatccattag aatcgggtgc gctagtcggc
      421 ctacgtgtct tggattttac cagtcgaatg ggaggatact gtggtctcct gctggcaaat
      481 cttggggcgg aggtacttct aattgaacca cccggtggcg atgccatgcg acgcgaaggg
      541 ccgttcaagg aggacagcct aaatccggaa ggaagcatct catttgccgc gtaccacaca
      601 aacaagcgag gaattgtcct cgatctagag tccgacggtg accgtgcaag cctgcggcga
      661 ctccttggca aggcagacgt gctcatcgaa gatcgtccgg tgggattttg gcaacggctt
      721 ggtcttggct accaagatct gcaagccatc aatccagcac tggtcatgac ttccatcagc
      781 gggtttggcg gcgcgggccc atatcgcgat ttcaaggcgc caaacatcgt cgcatttgcg
      841 atgggcggtt tgatgaatct atgcggccat cccgagcgcg cgcccctcag cggtccgtgc
      901 gatctcgctt atcgcctggg atccgtgcat gcggcgtttg gcaccttggt agccctgtta
      961 aaccgccgcg cctcgggtcg tggcgaccat ctagacattt cacttcagga cgtactggtt
     1021 gccgacccgt ttctccgcat catcacgcga tacagtgtca cgggtgaaat accgcagcgg
     1081 accggacaca gccaggccac gaccgttgct gaaacctacc agtgcaaaga cggttttgcc
     1141 cgcatcttct gcaaccaacc ggatcactgg cgacgcctgg ttgaatggct ggggaatccg
     1201 ccggagttga tcgatccgaa attcgagaac gtgcagaacc gattgccatt gcggccgctg
     1261 ctcgatcggc tcatcgaggc gcgcactttg aactatgacg tgaaaagttt cttcgacgag
     1321 tttcaaagcc ggcggctggc cgcggcgcca atcaactccc ccagcgcctt tctcgaagat
     1381 gaacagaccc gggatcggga ctatgttgta gaagttgatc acgctcattt cggccggcac
     1441 cgttttcctg gggatcctta taaattttct gaaagcccat ggcatatcac ccggggcgcc
     1501 ccgctcgttg gcgaacatca gtccatgata acgagcggat ggtcgcaacc ctcgccggca
     1561 ttgccatcgt tgaagccagt cggaagcgat agtcgagctt tcgcggggat tagagtcatc
     1621 agtttcccga ccggcattgt tggaccggcc ttgggctgct tactcgctga gcacggtgcg
     1681 gaggttattt ccatcgaagc cggccgtacc gtccgtagcc cgcagcgcgg ccagcgattt
     1741 caaattgtct ccgacctcga gagtaaccgc ggccgcaaac ggattgccat caacatgaag
     1801 cacagcgaag gagtggatat cgccaaacgc ctcatcgctc gatcggatgt tgtcgcggag
     1861 aatttcagcg cccgggtaat ggcgagttgg tcactggact atccgcgcct acgcgaagtt
     1921 cgtagcgaca tcattatggc cagccttcaa gctttcggtc agacagggcc gcggcgcgac
     1981 tatgtcagct tcggaccgat tctcatggct tactccggta tggcctatct ctggcgcgat
     2041 cccgagatcg agcggccggg tgccgcatgt caaaccgcct ttccggatta tatcgcgccc
     2101 tcctatggtg cgctggcgat tttagccgcg ctacactacc gtgcgcgtac cgggaaaggc
     2161 cagtatatcg atctctcaca ggcggaaacg gctgcatcgc tcatcgctcc ggcacatctg
     2221 gagtggctgc taactggacg ggagcctcaa ccgagtggaa atttcagcgc atcggccgcg
     2281 ccacacggct gctacaaatg caagggcgag gaccgctggt gcgtcatctc ggtgcaaacg
     2341 gaagaagaat gggcgcagtt ctgcgatctg gtcggccatc gcgagtggct tgcagatgct
     2401 cggtttacgg accttttatc ccgggttgcg aacggtccag agctcgatcg tttggtcgaa
     2461 tcatggacgg cgcggcatac gccgcatcag gtcatgatga ttttgcagcg caacggtgta
     2521 gcggccggtg tggtccagac tggcgaagat ctctaccgcg acccgcacct gcgcgagcgc
     2581 ggctcgaccc gggaggtgtt ccatcagccc attggctggg tgacgcgcgc cgggccgtca
     2641 gtgcggttat ccgaagcgaa atttaccggt aataatcagg cgcactttgc cggcgaggac
     2701 aacgaggcgg tatttggcga actattggga atgtccagcc agcagattgc ggatctcagg
     2761 caacgagagg tgttgcgcta gggcggcgcg atctcgagca gttggatcgt taccgtcttc
     2821 aacgctgcgg cgtctttttc gatctcatgc aattttcaaa agctttagct ctctcaccgg
     2881 cgccggccag cgcagcctgc cgctcacaat ccgtggtatc acgactcgag ctgctgggac
     2941 tcgccgttgt agtggtcttg tccctcgttt gagccggcgg cggcgggctg gcgcacgcag
     3001 ccgtcaacat cgccaaagca agcagtacaa tcttcatgcg tttcccccta acagaggtaa
     3061 gtgtcacaaa tgctttttcc gcgattaact ctcgcttaaa gaactctacc tcacgctctg
     3121 aggtttcttt catacctctt agttgctgga atgtcactgt ctgcatcgca gtttatcgag
     3181 cttaacagtt cttcccaacc aggttaaagt tttgcaagct tagaagcttg tcagttgctg
     3241 gtttagtttt ccatgctggg actagtcaag aatccacaag cggcatcaag tctgacggag
     3301 ccgacagcaa tccagcggta ggtggaggac tatcgaggta agagagcaca gcctggctcc
     3361 gcgaggggtt tcacgagatg aatcgaggcg gtctcttgaa tgctcgtttc aatccgatca
     3421 tgttaagaca cgccgatgct gtgcaatatg tgaatcatcc agtggaaagg gtggtccgaa
     3481 actggtcgtt ttaatggcca gcaactcgga gcacgtagat aattcaagga ggaacaatgg
     3541 gagcaattga tgctgatgct catgtgatcg agacggtgca gacctggagc tatctggaga
     3601 atgatgaacg acgtttcacc ccgcaactca tgactcagac cttcggtgag aaacttctca
     3661 gcaatgaagg aatcgccgtc cccgaattct gggtcattga gaaccgcgcc tttgggaaag
     3721 accgcaacgt cggcacggat acgtctaagg aatcgcgtga aatgctcagc gtccaggcgc
     3781 gcctcgcgca tatggatgag ctcggcatcg aaacgcaggt gctttatccg acgctgtttc
     3841 tccggccggt tctgcaggat gccgaacgcg agcgtgctct ctgcaaatct tataatcgct
     3901 ggatgggcga gctttggcgg caaggccagg gccgcttgcg ctgggtggcc aagcctccgc
     3961 ttcgtttact tgaaagaatg cccgccgcaa taaaacaaga actcgaatgg tccaaggaaa
     4021 acggcgcctg cggcatcttc atgcgcggct tggaatgtga gcgggcgctc ggcgctccct
     4081 atttctaccc gctctgggaa atggcagcgg aacttgatct tcctgtctgc attcattcgg
     4141 ccaacggtag ctttatccat catgagttct ttgccgatga cacatcgttc actaaattca
     4201 aactcactgt cgtcggcgct tttcatactt tgctggagaa ggagattcca aacaaatttc
     4261 aaaaagtacg ttggggtttt gtcgaggtca gcgcccagtg ggtaccctac gttctcaacg
     4321 atctagccga tcggtttagg cgtcagggcc ggccttttcc agccgacccg ctcggcgcca
     4381 acaacctgtg ggtagcctgc gaaaacaccg atgatctggc ttacgtccta cgccatgcgg
     4441 gtgaggactg tctgatgatc ggcaccgact atggtcacca tgatccttcc accgagctca
     4501 acgccattca tctgctgcgc aacgacaagc gaattgcacc taacgttgtc agcaaaatac
     4561 tggagacaaa tccgaagaag ttctacggct tgaattgaga acagaggcgc ggaactccgg
     4621 aaaataagct tacgagtgcc taccggtaag attgttcaaa caacaatgaa gcgcctgtaa
     4681 gagagggcca tgtcttgatc tagcgccgtt tctccagatc cttttaggcc tggtacttca
     4741 cctctccgcc caccagcgtc atctccactt tgagatcctt gatgcgctcc ggctgaacct
     4801 caaacggatc ctcggcaagg acggcgatgt cggcaagctt gccaacctcc aatgtgcctt
     4861 tgtcgttctc ctcgaaaccc acccaggacg ccgtcgtcgt gtgcatcgca aaggcttcgc
     4921 tgacgctgat tctttcctca ggcacccaca cgtcgcccca gcgcattttg cgtgacgcgg
     4981 cagtggcgat atcccgtaga ggatcgaccg gccagtagcc cggcgagtcg gagccgccgg
     5041 cgatgatgat gccgttcttc aagagcgttc gaaatggaaa agctttggtg agccgcctct
     5101 cgccgacgca atcgagaatc gcatcgccga tgtaataggc aaaagcgata tttggaatgg
     5161 cgacgatccc cgatcgtacc attcgctgca tgagcttcgg cgtagcgagc caattaccca
     5221 tgtgttcgat acgatgccga tgatccttgc gcggcgaatg ctcgatagca ttttcatagg
     5281 cgtccagcgc catatcgaaa gctttgtcgc caatggcgtg aacgcgg
//
LOCUS       DASUTG010000626         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1578003, whole genome shotgun sequence.
ACCESSION   DASUTG010000626 DASUTG010000000
VERSION     DASUTG010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578003"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..>2156)
                     /gene="dxs"
                     /locus_tag="VF089_14440"
     CDS             complement(326..>2156)
                     /gene="dxs"
                     /locus_tag="VF089_14440"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX6175207.1"
                     /translation="YILETMSRIGGHTGASLGAIELAVALHYAFDTPRDRLVWDVGHQ
                     AYAHKILTGRRDALPTIKQYGGISGFLRRDESEYDVFGAGHASTSLSAALGMAIARDR
                     KGENHHVVALIGDASLAGGMAMEAINQAGHLKSRLIVVLNDNEMSIAPAVGALTGYLN
                     RIRGAHGYHRFKDEVEETLRAIPSVGERLHHAAKTVKDAIAAAVLPGALVNELGFKYI
                     GYVDGHNPRALVAAFREAQQVQDGPVIVHALTTKGKGYPEAEKDYYRWHATGPFDLQS
                     GQAIKSKPGAPTYTAVFGDTLAQLMARDEKIIALTAAMPDGTGVCTPLEKFPERSFDV
                     GIAEQHCVTFAAGLSCEGLKPVCAIYSTFLQRGFDQLVHDVCIQDLNVKFCLDRGGVA
                     GGDGPTHHGLLDIAYLRGIPNIVLMAPKDEGEMRDMLYTMLEHVGPAAMRYPRGSGLG
                     VPIDTEPKLLEIGKGEILRDGGEIAILAYGSMVHPSLQAAEHLAKEKIEATVVNARFV
                     KPLDAQLLLALAQTKRLIVTVEEAYLSGGFGSAFLELLEENGLQDKVRVVRMGIPDRL
                     VSHGDPKLLLAKYGLDADGIYTRVKQSIDVLDDRRAKPQKLGR"
BASE COUNT          461 a          640 c          568 g          487 t
ORIGIN      
        1 cagcccgcct ttagaacgtc tggtcacgct cacttcctcg agcggaacaa ctggacgagc
       61 cgcgaggatc gtcgcctcct catcgaagtg aggttcgtca agattcattt gatcatcaat
      121 atttgacttc atttatccac ccggttccct gcgccgacaa ctgtccaacg ctggtagtag
      181 caagcgcaag gccacaataa gattcgccca aaagctttgc ttttttggtt ccgcgactaa
      241 gtgaattcac ccatatcgtg caccaaaaca acgcgcccgt ctgtatttgc agtacgggcg
      301 ccgcgagttt gttatgtggt gatttttacc ggccgagctt ttgaggcttc gcgcgccgat
      361 cgtcgagtac gtcaatgctt tgcttcacgc gcgtatagat accatcagcg tcgagtccat
      421 acttcgcaag cagtaacttt ggatcgccat gggaaaccag ccgatcggga atacccattc
      481 tgaccacgcg aactttgtcc tgcagtccgt tttcttccaa caactccagg aacgccgagc
      541 cgaaaccgcc ggaaagataa gcctcttcga cagtcacgat cagtctcttg gtctgggcca
      601 gtgccagcaa cagttgcgca tcgagaggtt tcacaaatcg cgcgttcacc acggtggcct
      661 caatcttttc ctttgccagg tgttccgccg cttgtaatga aggatggacc atggagccgt
      721 atgcaagaat tgcgatctcg ccgccgtctc tcagtatttc tcctttgccg atttccagta
      781 atttcggttc agtatcaatg ggaaccccta gaccgctccc gcgcgggtaa cgcatggccg
      841 cgggaccaac gtgctccagc atggtgtaaa gcatgtcccg catctcgcct tcatccttcg
      901 gcgccatgag cacgatgttg ggtatcccgc gaagatacgc gatgtccagc agaccgtggt
      961 gtgtcggccc gtcgccgccg gccacgccac cccggtcgag acaaaacttc acgttcagat
     1021 cctgaatgca gacgtcgtga acgagttgat caaagccgcg ctgcaggaat gtcgagtaga
     1081 tcgcgcagac tggttttaaa ccttcacagg aaagaccggc cgcgaaagtc acgcaatgtt
     1141 gttcagcaat tccgacgtca aacgaacgct cgggaaattt ttcgagtggt gtacaaactc
     1201 ccgtgccatc aggcatagcg gctgtaagcg caataatttt ttcatcgcgg gccattagct
     1261 gggcgagagt gtcgccgaat acggccgtgt aagttggcgc gccgggtttt gacttaattg
     1321 cctgtccact ttgcaaatca aacggaccgg ttgcatgcca gcggtaataa tccttctccg
     1381 cttccggata acctttgccc ttggtggtga gcgcgtggac gatcactggg ccatcctgca
     1441 cctgttgcgc ctcgcgaaac gccgccacga gggcgcgagg attgtgaccg tcaacatagc
     1501 cgatgtattt gaaaccgagc tcgttaacca gagcgccggg aagcaccgcc gccgcgatcg
     1561 catcttttac ggtcttggct gcgtgatgca gccgttcgcc taccgaagga atcgcccgca
     1621 gcgtttcttc aacctcatct ttgaagcgat ggtagccgtg cgctccccga atacgattta
     1681 agtatccggt cagtgctccc acggcgggag cgatcgacat ctcgttgtcg ttaaggacca
     1741 caatcagccg gctctttaaa tgacctgcct ggttgatcgc ttccatggcc atgccgccgg
     1801 caagcgaagc atcaccaatc agcgccacca catgatgatt ctcgcctttg cgatctcggg
     1861 cgatggccat gcccagcgcc gccgataatg acgtcgaggc atgaccggcg ccgaacacgt
     1921 cgtattcaga ttcgtctcgc cgcagaaaac cgctgatgcc gccgtactgc ttgatggtcg
     1981 gcagcgcatc acggcgcccc gtaagtatct tgtgcgcgta cgcctgatgt ccgacgtccc
     2041 aaaccaaccg atcgcgcggc gtgtcaaacg cgtagtgcaa tgcgactgcc agctcaatgg
     2101 cgcctaagga agcgccggta tggccgccga tgcgggacat ggtttccaga atgtat
//
LOCUS       DASUTG010000627         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_541189, whole genome shotgun sequence.
ACCESSION   DASUTG010000627 DASUTG010000000
VERSION     DASUTG010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541189"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VF089_14445"
     CDS             complement(<1..497)
                     /locus_tag="VF089_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX6175208.1"
                     /translation="MIIIPAIDLKHGRCVRLFQGEMDQETVYFEDPLDAARHWLDEGA
                     TIIHIVDLDGAVEGRPVHMRQVEAICRETSLAVEVGGGLRSMETVEAAFELGVDRVVI
                     GTEAYSNTGFLRLLCEKFPGRIVAGIDARKGKVAVKGWKETTAMDAVELAKRCEQAGA
                     ARIIYT"
     gene            complement(501..1118)
                     /gene="hisH"
                     /locus_tag="VF089_14450"
     CDS             complement(501..1118)
                     /gene="hisH"
                     /locus_tag="VF089_14450"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6175209.1"
                     /translation="MIAIIDYGMGNLRSVQKAFERVGYEAVVTREAGQIESARGVVLP
                     GVGAFSACMENLGRFGLIEPICDIVRRKKPFLGICLGFQLLFSESEEFGRQKGLDLLP
                     GKVVGFHSKRGLKVPHMGWNTLHKKKDSAFLEGVPSGEFVYFVHSFYVVPEDASVIAT
                     DTEYGSAFVSSIATDTLFACQFHPEKSQEIGLRILSNFGRFVASN"
     gene            complement(1184..1771)
                     /gene="hisB"
                     /locus_tag="VF089_14455"
     CDS             complement(1184..1771)
                     /gene="hisB"
                     /locus_tag="VF089_14455"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX6175210.1"
                     /translation="MGRTGAVERKTKETEIVVAVELEGSGKAEIETGMPFFNHMLDSF
                     SRHGLFDIKLQARGDLEVDYHHTVEDVGLALGEAFKKALGDKHGIRRFGEASCPLDET
                     LAKVVIDLSGRPYLSYNVKIRPGRVGSFDTDLPHEFYAAFTNQLGMNLHIDVIRGENP
                     HHIIEACFKAFARAMDMATGFDERISGVLSTKGSL"
     gene            complement(1720..3069)
                     /gene="hisD"
                     /locus_tag="VF089_14460"
     CDS             complement(1720..3069)
                     /gene="hisD"
                     /locus_tag="VF089_14460"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6175211.1"
                     /translation="MKVRIYKTFEPSYHSFIRRILARRGGRQGDVEQRVDEIIRTVQQ
                     QGDRAVLRYTRDFDRRRMTRASMEVTTGEIDRAMAKVPAKDLGTLRLAAKRIAAFHQR
                     QLQKSWQYRDPLGMVLGQRITPLERVGVYVPGGKASYPSTVLMNVIPAKVAGVKQIVM
                     TSPIGNDADIILAAAKIAGVDRIFRVGGAQAIAALAFGTATIPKVDKIVGPGNIFVAT
                     AKRLVFGEVNIDSIAGPSEILLLADDSADPAHVAADMLSQAEHDELAAALCITPSMSA
                     ATTIQKTLEEQLRRSQRHNITMKSLENYGAIIVTADLTEAVRIANQIAPEHLELLVRQ
                     PEKWALEIRNAGAIFIGPYSAPPLGDYLAGPNHVLPTGGSARFFSPLGTYDFLKRTTV
                     IHAEKRALRALAPKIIQLARLEGLDNHARAVEARFVKEGEQWDAPERLNVKPKKQRL"
     gene            complement(3120..>3467)
                     /locus_tag="VF089_14465"
     CDS             complement(3120..>3467)
                     /locus_tag="VF089_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6175212.1"
                     /translation="TDLQAQMMVLMSVADGVSVITETIFENRFMHAQELDRMGAQIQL
                     EGNRAVVRGARELSGAPVMATDLRASISLILAGLVASGSTEVSRVYHLDRGYEHIEEK
                     LSNLGAQIERVKA"
BASE COUNT          792 a         1016 c          859 g          800 t
ORIGIN      
        1 gtgtaaataa ttcgcgcggc accggcttgc tcacagcgtt tggctagctc gacggcatcc
       61 atcgcagtcg tttccttcca gcctttgacg gcgactttgc ctttgcgcgc gtcgataccg
      121 gcaacaattc ggccgggaaa tttctcacac agcaaacgta gaaagccggt attactgtaa
      181 gcttcagtac cgatcaccac tcgatcaacg ccaagctcga aggctgcttc gacagtttcc
      241 attgatctaa gcccgccgcc aacttctact gcgaggctag tctcccggca tattgcctca
      301 acctgccgca tatgaaccgg tcggccttcg accgcgccat ccaagtcgac gatgtgaatg
      361 atcgtcgcgc cctcgtccaa ccaatgccgc gcagcatcca atggatcttc aaagtaaacc
      421 gtttcctgat ccatttctcc ctggaacaaa cggacacaac gaccatgttt aagatcgatc
      481 gccgggataa tgatcatgaa ttaattggaa gcgacgaagc ggccgaaatt agaaagaata
      541 cgcagaccga tttcctggct tttctcggga tgaaactggc aggcaaaaag cgtatccgta
      601 gcgatgctgg aaacaaacgc gctgccatat tcggtgtcgg tggctatcac cgaagcgtct
      661 tccggaacga catagaagga gtggacaaag taaacaaatt caccgctggg aactccctcc
      721 aagaaagctg aatctttttt cttgtggaga gtgttccaac ccatgtgcgg cacctttaaa
      781 cctctcttag aatgaaagcc gacgaccttc ccgggaagca aatcgagccc cttctgccta
      841 ccgaattctt cgctttctga aaacagtaac tgaaatccca aacagatacc caaaaacggc
      901 tttttgcggc gtacgatatc gcaaataggc tctatcaaac caaaccggcc gagattttcc
      961 atacaggcgc tgaaagcgcc aacgccgggc aagaccacac cgcgagcgga ctcgatctgg
     1021 ccggcttcgc gcgtgaccac agcctcataa cctacccgct caaacgcctt ctggacgctg
     1081 cgcaggttac ccatgccata atcaatgatc gcaatcatag acttttctct atttatttgc
     1141 cggatctgac gatctaaagc acttcaacga cttggcatta ttactagagg ctacccttgg
     1201 tcgacagcac tccacttatc cgttcgtcaa aaccggtggc catgtccatc gcgcgagcga
     1261 acgctttgaa acatgcctca ataatatggt gcggattttc gccgcgtatc acatcgatgt
     1321 gcagattcat gcccagctga ttggtaaacg cggcatagaa ctcatgcggc aggtcggtat
     1381 cgaaactgcc gacgcgacca ggacgaattt tcacgttgta cgaaagatag ggccggccgc
     1441 tcaagtcgat aaccactttc gccaaagtct catccagcgg gcagcttgcc tccccgaacc
     1501 ggcgaattcc atgtttatcg cccaacgctt tcttgaatgc ctctcccaag gccaggccga
     1561 catcctcgac cgtgtggtga taatcaactt ctaaatcgcc cctggcttga agcttgatgt
     1621 cgaacaaccc gtggcgagag aagctatcca acatatgatt gaagaacggc atgccggttt
     1681 cgatttccgc tttgccgctg ccctccaact caaccgccac tacaatctct gtttctttgg
     1741 ttttacgttc aaccgctccg gtgcgtccca ttgttcaccc tccttcacga atcgcgcctc
     1801 cacggcgcgg gcatgattat cgagcccctc gagtcgcgcc agctgaataa tcttgggagc
     1861 caaagcccgc aaggcgcgtt tttcggcgtg aatcaccgta gttcgtttga gaaaatcata
     1921 ggtgcccagt ggagagaaaa agcgcgcgct gccgcccgtc ggaagcacat gatttgggcc
     1981 ggcgagatag tctcctaacg gcggcgccga ataaggccct atgaagatag ctccggcgtt
     2041 gcgtatctcc aatgcccatt tttccggctg tcgaacgagt agctcgagat gctccggcgc
     2101 aatctggttg gcgatcctca cagcttcggt caaatcggcg gtaacaatga tggcgccgta
     2161 attttctagc gacttcattg taatattgtg tcgctggctc cggcgtagct gctcttcgag
     2221 cgtcttctgt atcgttgttg cggcactcat cgacggcgta atgcatagag ctgcggcaag
     2281 ttcgtcatgc tcggcctggg aaagcatgtc tgcggcgaca tgcgccggat cggcagaatc
     2341 gtcagccagc agcaagatct cactgggccc ggcgatcgaa tcgatgttga cttcaccgaa
     2401 gaccagccgc ttggcggttg cgacaaagat atttccgggc ccaacgattt tgtctacttt
     2461 gggaattgtc gcagtaccga acgctaaagc cgcaatcgcc tgagcgccgc cgacgcgaaa
     2521 aatccgatcg acaccggcaa ttttggcggc ggctagaatg atatccgcat cgttgcctat
     2581 gggcgaagtc ataacgattt gtttaacccc tgccactttc gccggtatta cgttcatcag
     2641 cacggttgag ggataagagg ctttgccgcc gggaacataa acgccgaccc gctccagcgg
     2701 agtaattctt tgacccagaa ccattcccaa tggatcgcgg tactgccagc ttttctgcaa
     2761 ttggcgctgg tgaaacgcgg cgatcctctt agcggctaaa cgcaaagtgc cgagatcctt
     2821 ggcaggcact ttggccattg ctcggtcgat ctcgccggtt gttacttcca tactggcgcg
     2881 agtcatgcgc cttcggtcaa agtcccgagt gtaacgcagc accgcgcgat caccttgttg
     2941 ctgcactgtc cggatgattt catcgacgcg ctgctcgacg tcgccttggc gaccgccacg
     3001 ccgtgcaaga attcgcctga tgaacgagtg atagctcggc tcgaatgttt tatagatcct
     3061 aactttcaat acaagcgatt ctttctcgca gccgcatcga actcccgttg gtttgtcact
     3121 tacgctttga cccgctcgat ctgcgcccca aggttcgata gtttctcttc gatgtgttca
     3181 tagccgcggt ccaagtggta caccctggag acttcagtcg acccgctggc cacgagtccc
     3241 gcgagaatga gcgaaatgct ggcccgcaaa tccgttgcca ttacgggcgc tcccgacagc
     3301 tccctggcgc cgcgaaccac ggcgcgattg ccttcgagct ggatctgggc tcccatacgg
     3361 tccagttcct gcgcatgcat aaagcgattt tcgaaaatcg tttcggtgat gacgctaacg
     3421 ccgtcagcca ccgacatgag aaccatcatc tgagcttgta ggtccgt
//
LOCUS       DASUTG010000628         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1133641, whole genome shotgun sequence.
ACCESSION   DASUTG010000628 DASUTG010000000
VERSION     DASUTG010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133641"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF089_14470"
     CDS             <1..638
                     /locus_tag="VF089_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175213.1"
                     /translation="MGNHVHVILDNQPYEAYYSLDQPFELRNVVAGKHTLRVFPSRPW
                     HESYKNDGAFQVVTFTVTGGGDASKPTTTNTGQVVANNNASPAASPTSSPAAEGKDMP
                     ASTAGNVDLSKPLLTYSRPKGENKGPDADPIMIDFWLTNAKLRGDGGEYRVRYIIDDD
                     EPRFIDKWEPIWLSGWTTGPHTVRLELLDKDNNPVENGGYNTTSREITVVK"
     gene            691..1101
                     /locus_tag="VF089_14475"
     CDS             691..1101
                     /locus_tag="VF089_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6175214.1"
                     /translation="MIGDKWTLLVARDLANGPKRTMELHSGLFPISSRTLVGRLRDME
                     KDRLVLRKDFGGNPPHIEYELTERGRLLLPLVDALREIGESLGCNECEDRKKHLGFYC
                     EACPRNYAVTPVEPLRRSVPANDRRRRDDSIVLL"
     gene            complement(1123..2211)
                     /gene="trxB"
                     /locus_tag="VF089_14480"
     CDS             complement(1123..2211)
                     /gene="trxB"
                     /locus_tag="VF089_14480"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6175215.1"
                     /translation="MNPKQTIDKEVVIIGSGPAGLTAAIYSARANLSPLLIDAPADAE
                     KQTTPGGQLMITTEVENYPGFAEGIQGPDLMIEFRKQAERFGTEFLDAWINKVDLSER
                     PFLLETDEHRISTQTLIIATGASAKWLGIPGEAKVPNGFGGNGVSACATCDGPLPAFR
                     NKPLVVVGGGDTAMEEATFLTRYASKVFVVHRRDKLRASKIMQDKAMKNEKIEFIWDT
                     AVEEILGKPEEGVTAVRLRNLKTGKEGMLPCSGVFVAIGHKPNTDLFKGQLDMDEIGY
                     ILTSGKSTATNVPGVFAAGDVQDNVYRQAVTAAGTGCMSALDAERFLDHLPVAMPSGE
                     EVTMEGEHITADHKAIIEADGSMIPNQP"
     gene            2409..3302
                     /locus_tag="VF089_14485"
     CDS             2409..3302
                     /locus_tag="VF089_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6175216.1"
                     /translation="MRRPQTIETVGVIGAGTMGHGIAQVAAGAGFRVVLRDVDRESLA
                     RGVRAIERNLAKGMELGKLTEADCDSTLQHIHGTTHLEELADADLIIEAAPEILDLKQ
                     DLLREVEAIGKEGFIFATNTSSLSISEIARASQAPANVVGLHFFNPVHLMRLVEIVVG
                     KATSDDTVATVREVSHKLRKEPIVVNDVPGFASSRLGVALGLEAMRMLEQGVASARDI
                     DTAMELGYNHPMGPLKLTDHVGLDIRLNIAEYLYRELGSESFRPPEILRRMVAEGKLG
                     KKSGQGFYDWTEERQKSARIA"
     gene            3311..3703
                     /locus_tag="VF089_14490"
     CDS             3311..3703
                     /locus_tag="VF089_14490"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX6175217.1"
                     /translation="MNVPNNNEERRFALRMALRLPIVISGRAEDGSAWSEPTETDDIS
                     TSGALFHLNQKVSRGDRLYLRAHRPDGSPIEVTAVVIRIAPAIYGTARVGVQIAESTE
                     NWLRLFVSWVADDQPGSETAADADTTSE"
     gene            3902..>4401
                     /locus_tag="VF089_14495"
     CDS             3902..>4401
                     /locus_tag="VF089_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175218.1"
                     /translation="MKRCPQCEFIYEDEQVTCDMDGAMLALDRNVASINGQRSMVRSF
                     VVPAAVGIALAVVVCLAFYSSPLLIAKPDARQLSPAQMSAPAPTQPAIDVPSPTPSPE
                     AVPEPDGEEQAASPKRASDANHANRSTSDSRLTIRRGVPPLPRVPTLPRLPPARVRKS
                     SNDNVQV"
BASE COUNT          991 a         1172 c         1221 g         1017 t
ORIGIN      
        1 gtatgggaaa tcacgtccac gtgattctcg acaaccagcc atacgaagcg tattacagcc
       61 tcgaccagcc atttgagttg cgtaatgtag ttgccggcaa acacactttg cgcgtgttcc
      121 cgtcgcggcc ctggcatgag agctacaaaa atgatggcgc gtttcaagtc gtaaccttca
      181 ccgttaccgg cggtggtgac gcttcgaaac caacaaccac taatacgggc caggtggtgg
      241 cgaataacaa cgcctcaccg gcagcgtcgc caacgtcgtc accggcggcc gaaggtaaag
      301 atatgccggc aagtaccgca gggaatgtcg atctcagcaa gcctttgcta acgtacagcc
      361 ggcccaaggg tgaaaacaaa ggtcccgatg ccgatccaat aatgattgac ttctggctga
      421 ctaatgcaaa gttaagagga gatggcggcg agtatcgggt tcgttatatc atcgacgacg
      481 acgagccacg atttatcgac aagtgggagc cgatttggtt gtcgggatgg accacgggtc
      541 cacataccgt gcggctggag ttgttggaca aggacaacaa tccggtcgaa aacggtggtt
      601 acaacacaac cagccgtgag attaccgtag tcaaataaac tagtgagacg acgcaagatc
      661 gatggatgtc cgatagccgg tgcactacag atgatcggcg ataagtggac cttgctcgtc
      721 gcccgggatt tagctaatgg cccgaagcgc actatggagt tgcattcggg ccttttccct
      781 attagcagtc gcactctggt tggccgtctg cgcgatatgg agaaagaccg gctggtattg
      841 cgaaaggact tcggtggtaa tcccccgcac atcgaatacg aactgacgga gcgcggccgg
      901 ctgcttctgc ccttagtgga tgcgttaaga gaaattggag aatcgcttgg gtgtaacgag
      961 tgtgaggatc gtaaaaagca cctcggcttt tactgcgaag cgtgtccgcg caactacgca
     1021 gttactccgg ttgagccgct acggcgttca gttcccgcca acgatcgtcg cagacgcgac
     1081 gattcaatcg tgttgctcta aaccttaatc ttcctgcagc agctacggtt gattcggaat
     1141 catgctgccg tcagcctcta tgatcgcctt atggtccgca gtgatgtgtt cgccttccat
     1201 ggtaacttcc tcaccgctgg gcatcgctac tggtaaatga tcgaggaacc gttcggcgtc
     1261 gagcgctgac atgcatccgg ttcccgccgc agttaccgcc tgacggtaaa cgttgtcctg
     1321 cacatcgccg gctgcgaata caccagggac attggtcgcc gttgatttgc cggacgtgag
     1381 tatgtagcca atttcatcca tgtccagctg gcctttgaag agatcggtat ttggcttgtg
     1441 tccgatggct acgaatacgc cggagcacgg aagcatccct tccttccccg tttttagatt
     1501 tctcagccgc accgcggtaa cgccttcctc gggttttccg agaatctctt cgacggcggt
     1561 gtcccagata aactcgatct tctcgttctt catcgccttg tcctgcatga tttttgacgc
     1621 acgcagtttg tctcgacggt ggaccacaaa aactttcgag gcgtagcgcg tcagaaaggt
     1681 cgcttcttcc attgccgtat cgcctccacc taccacgacc aacggtttgt ttcgaaacgc
     1741 cggcaatggc ccatcacacg tagcacaagc tgaaacgccg ttgccgccga agccgtttgg
     1801 aacttttgct tcccccggaa tacccagcca tttggctgat gcgccggtgg cgatgattaa
     1861 cgtttgggtg ctgatccgat gttcgtctgt ttccaggagg aatgggcgtt cgcttaaatc
     1921 gactttgttg atccaggcgt cgaggaattc cgtaccgaag cgttccgcct gcttgcgaaa
     1981 ttcaatcatc aagtccgggc cctggatgcc ttcagcgaaa cccgggtagt tttccacttc
     2041 ggtagtaatc attaattgtc cccccggggt ggtctgcttt tcggcgtcgg ccggcgcatc
     2101 aattaagaga ggactgaggt tagctcgggc agaataaatt gctgccgtta gtccggcggg
     2161 gccggaaccg atgataacga cttctttgtc gatcgtttgc ttggggttca ccgtcttatc
     2221 cattctttta gggtagtttc aggggtgcaa tcaggcacgg gcattggtgt aatataggcc
     2281 tcaaaattgg ccgaatgtat agccttttcc gttagtacat agtataacta ccgctactaa
     2341 cgtagtcgaa cgcgagagtc gtaagcctta ctcagatcga ctcaactgac aggattttga
     2401 ataaaagctt gagacgtccg caaacgatcg aaaccgtcgg tgtgatcggc gccggcacta
     2461 tgggtcacgg gattgcgcag gtagcggcgg gagcgggctt tcgagtggtg cttcgcgacg
     2521 ttgaccggga aagtctggct cgcggggtaa gagcgatcga acgcaatctg gctaagggga
     2581 tggagctcgg caaactcacc gaagctgact gcgattcaac gctccaacat attcacggca
     2641 ctacccacct cgaagagcta gctgatgctg atctgattat cgaagctgcg ccggagattt
     2701 tagatctgaa acaagatttg cttagggaag tagaagccat tggcaaggaa ggtttcatct
     2761 tcgcaactaa cacgtcctcg ctttcgatct cggaaatcgc gcgggcctcg caagcgcccg
     2821 ctaatgtcgt tggcctgcac ttttttaacc cggtgcacct gatgcggctt gtggaaattg
     2881 tggtgggcaa ggccacaagt gatgatacag tggcgactgt tcgcgaagtc agtcacaagc
     2941 tgcggaagga gccgattgta gtcaatgacg ttccgggttt tgcatcgagc cgtttgggcg
     3001 ttgccctcgg cctggaagca atgcgaatgc tggaacaagg cgtggccagc gcacgggata
     3061 tcgataccgc aatggaactg gggtacaacc accctatggg accgcttaaa ctgacagacc
     3121 atgttggtct ggacattcgt ctcaacattg ccgagtattt atatcgtgaa ttgggatccg
     3181 aaagttttag gccgcccgaa atcctgaggc ggatggtcgc tgaaggtaag ctgggcaaga
     3241 aatcaggcca gggtttttat gattggacag aagagagaca gaaatcagcc cggatcgcct
     3301 aactgagcgg atgaacgtac ctaacaacaa cgaggagcgc cggtttgcgt tacgcatggc
     3361 gttgcgtctt ccgatcgtca tctcgggacg tgctgaagat ggttccgcct ggagtgaacc
     3421 aaccgaaact gacgacattt caacctccgg cgcgttgttt catctgaacc aaaaagtcag
     3481 ccgtggcgat cggttgtacc tgcgagcgca ccgtccggac ggcagtccta ttgaagttac
     3541 tgccgtcgta attcgcatcg cacccgcaat ttatggaaca gcacgcgtcg gcgtccagat
     3601 tgctgaatca actgaaaact ggctaagact tttcgtgtca tgggtggctg atgatcaacc
     3661 cggctccgaa accgctgccg acgctgatac cacttccgaa taacagcgcg ccataggctt
     3721 caaaaggctg cgtttttgca gccttttttc atttttgcgc gcccggcaga ttgactgttt
     3781 accagtctat gtcccaagca gtattctgta gtcacactca ccagtagccg agacggcgcc
     3841 ccaacatcac aagttcaatc aagaccggcg agcttctcga tctgtcttcc ggagggatgc
     3901 gatgaaaagg tgcccgcaat gtgaattcat ctacgaagac gagcaagtaa cctgcgacat
     3961 ggacggcgcc atgctggctc tcgataggaa cgttgcgagc attaatggcc agcgatcgat
     4021 ggtgaggtcg ttcgttgttc cggccgcggt cggaatcgcg ttggccgtgg tggtttgcct
     4081 ggcgttttat tcgtcgcctt tgttaattgc gaaacccgat gcgcgtcaac tatccccggc
     4141 gcagatgtcc gctcccgcgc cgacgcagcc ggctatcgac gtaccttcgc ctacaccatc
     4201 gcctgaagcg gtgccggagc cagacggtga ggaacaagct gccagcccca aacgagcgtc
     4261 ggacgcaaat catgcaaatc ggtcaacgtc ggatagcagg ttaaccatta ggcgcggcgt
     4321 gcctccattg ccgcgagttc ccacgctgcc gcgccttcct cccgcccgtg taagaaagtc
     4381 gtcgaacgac aacgtccagg t
//
LOCUS       DASUTG010000629         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1877991, whole genome shotgun sequence.
ACCESSION   DASUTG010000629 DASUTG010000000
VERSION     DASUTG010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877991"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..598
                     /locus_tag="VF089_14500"
     CDS             83..598
                     /locus_tag="VF089_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6175219.1"
                     /translation="MRIRNNGRNTLLTAFLVIAAQFLPQDQAHAQAQPVSIFVVRHSE
                     TDSSQPALPLTAAGRQRAELLVQTLRAVRFTHIFASHTTRSRQMVAAIASAQGLAVIQ
                     LPVPGSTLDAEPVTEQTSRRAPIEPISTALLNLPPGSVALAALNSENIFAILNRLGIR
                     VAAGGQSCTPG"
     gene            2614..3054
                     /locus_tag="VF089_14505"
     CDS             2614..3054
                     /locus_tag="VF089_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6175220.1"
                     /translation="MQEFDSLFGDTPLAQITTWQIEKWKSDKGKTVQRQTVNRSLTVI
                     KHMFKKAVDWSRTKTDPASGVKRYKVVSERTRFLSAADVQTILQKCKEDLTSPWLYPL
                     VKLALNTGMRQGELLRLKWEGINFEPRHDQRPTNQNNAVEDHRH"
     gene            3150..3320
                     /locus_tag="VF089_14510"
                     /pseudo
     CDS             3150..3320
                     /locus_tag="VF089_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3535..3610)
                     /locus_tag="VF089_14515"
     tRNA            complement(3535..3610)
                     /locus_tag="VF089_14515"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3575..3577),aa:Cys,seq:gca)
     gene            3720..>4064
                     /locus_tag="VF089_14520"
     CDS             3720..>4064
                     /locus_tag="VF089_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEX6175221.1"
                     /translation="MATMITSECINCGACEPECPNNAISQGDPIYVIDPLLCTECVGF
                     HDFEACAAVCPVDCCVTDPNNIESEEVLIGRAKALHPETSFDDDFQSRFRKANGAQID
                     VTISASERNEVPP"
BASE COUNT         1035 a         1010 c         1109 g          910 t
ORIGIN      
        1 taaactcccg ggttagatgg aggcggacat cgctcacccg aaacgattcg cgccgccgtc
       61 attaacacac tggaggtgct acatgcgcat tcgcaacaat ggtcgcaata ccctgctcac
      121 cgcatttctc gttatcgccg cgcaattctt gccgcaagat caggcgcacg ctcaagcaca
      181 gccggtttcc attttcgtgg ttcgacattc cgagacggat agttcgcagc cggcgttgcc
      241 gctgactgcg gcagggcgcc agagagcgga gttattggtt cagacgctac gcgcggtaag
      301 gtttacgcat atcttcgcga gccacaccac ccggtctcgc cagatggtcg cagccattgc
      361 gtcagcgcaa ggcctcgcgg tgatccagtt gcctgtgccc ggctcaaccc tcgacgcgga
      421 accggtgacg gagcaaacct ctcgccgagc cccgatagag ccgatctcaa cagccttgct
      481 taacttgcca cccgggagcg tcgcgcttgc ggcgctgaac agcgagaata tttttgcaat
      541 tctgaatcgg ctgggcatcc gcgttgcggc gggtgggcag tcctgcactc ccggttaaaa
      601 cgggcgtcgc gtacagtacc ctgatgaagc gcctgaactc tttcaaagca gaggacaagg
      661 tggactttga gaagggcggg ggaaaagggt ctgtactcct gtggtttcaa atcgaggttg
      721 ctcttcaaat aacccgcaat agacgaggcg agttaatcgg cttgccgggg tggtgcccgg
      781 tcgagaaggg ctgcgctcgg ttgacgggca aagccaagga ctcgatgggg gtggcaagtg
      841 cgctgccccc gttgggtccc acaaagggct aggtgagctg atcagcattc aagtccaaac
      901 agcagtgaga aaacgcaacg cgtggtgcca cggttcagac agaagctgag catgatgaga
      961 ttctggcgat tgctaactgg gaaaaactca cggggtgtga cgtgttgttg ggactcaact
     1021 cgaggacccc atcatcaatt gttgggcttg gtagctcatt cctttccacg cggcagtaat
     1081 gaaggaaacg atatgtcatt agttagtctg ttctattttc ctcgtacgct cgaaggtatt
     1141 ccggtaggaa accgccccag gtaaatttgg tgcatttgca tcctttcacg agacacgccc
     1201 cttggccgtt agttccgcga tgatgagagt ggggtccgtc ggcgttgtga ctgcaggtgc
     1261 aaacgcttct tggctttgga taacgtctta ccgtcggtct cgcttttggt gccatcagag
     1321 atgcaacccg agctatgcgc tctaattaga aagcagtaag taagaaatac acccctagga
     1381 aaattatgat ggaaacaaag accagtactg aatcttccgc gtacttggac cagaaggccc
     1441 ggaacttgtt catggtgcca agttctaacc gcaagaacga gacccatcca aattattccc
     1501 aattatcctc cagggggcac gtatggcgat ttttgacaat agttatcagc taacaatgac
     1561 agacacgtca cgccagtcgc tacctcaaca gagatggagg ctgtccaatg aaccaacgcg
     1621 ccaaacgagc tgatgagcaa cagcggtaga ataggctcgg ttgtgccgcg ctgcaggttg
     1681 cgacggtatt ttgcatgagg cgtgagatgg cgaggcagca atagactcgt gatcgctaga
     1741 gatcgccaac tattaaaagg cattcgctag atgaagcaat catccaccca acgggcttga
     1801 ttcaactctg ctcgtcgcta tgaccattgg acagctccgg gacctcatga ggcaggaact
     1861 gaaagccatc gtggcaggcc ctgcgaacag ccgcaggcca gctgtcgaca aaggtacttc
     1921 accgtgaaag aggctgccga atgttccggg ccaggtgcct cgacgattcg gcttcagatt
     1981 tgacggaagc agctgttagc ccaacgcgtg ggccgtaagg ttctcatgaa acggtccgat
     2041 ttagattttt ttctgcagtc ccgtccagtg ccgcaccata cagttaacca tacctagtct
     2101 tacgggctat agttgtttta actatgacgg gttccgagtt caaacggcta cgtacatcta
     2161 tggggcattc gcaagcccgt tagcccgaga gctagaccta tatgtgagaa ccgtctcgaa
     2221 atacgagaat gatgaccttg tgattccaaa agtaacggag cttgcacttc ggtatttggt
     2281 acagcaggct aaaccaagaa gagaggcgct aacacggcta aggcgaacgg tatctacgag
     2341 cgcaaaggca ggcatggggg acgttacctt ctacattcta tcgattcgag ggcactgaca
     2401 taaaggaaca tgtcggcaga aaatcacggg gatttaaccg tgagttggca agagaagcac
     2461 tgaaatctcg cctgggtgac taggcgagag ggcactttaa tttggagaag acgcgaaaac
     2521 ccgttccatc tccaagctgg cggaacgcta ccgcgaattt gccagtggct ataaacgggg
     2581 ctggtatggg gctggtatga gaaaaagtac atcgtgcagg agtttgacag tctctttggc
     2641 gatactccct tagctcagat cacgacttgg cagatcgaga agtggaaatc agataaaggc
     2701 aagacagtac agcgacagac cgttaaccga tcattgaccg tcattaagca catgttcaag
     2761 aaagctgttg attggagcag gaccaagacg gatccggcaa gtggagtgaa gcgctataaa
     2821 gtagtttcag aacggacccg gttcttatca gccgctgatg ttcagacgat tttgcagaag
     2881 tgcaaagagg atcttacttc gccttggctt tatccgttgg tcaagttggc gctcaatacg
     2941 ggcatgcgtc aaggagaatt gctccgtctt aaatgggaag ggattaattt cgaaccgagg
     3001 cacgatcaac gtcctacaaa ccaaaacaat gcggtggaag accatcggca ttagcgcgga
     3061 tgcgttagag gctttaaact ggcttcagtc aaaccgatac ggcgattact gtttatgtgg
     3121 ccgtgggcga tatagtcggc aagacagccg tgtacgatgc ttttaaacgc gcctgcaagg
     3181 ccgcacgaat tgaagatttt catttccatg accttcggca taccgttgct tcacatctca
     3241 tcatgggcgg agttgatttg ttcgccgtga aggaattgct aggtcacaaa cagatcaatg
     3301 agacggtacg ctactcccac ctcgcgccag aacataccgc ccacgccgtg gctaagttga
     3361 ccgttaagtt caagccgtca gaggccgtgc agcttgtgaa aaccggaact tcttcggatc
     3421 aaagggaaga ggtaaactca gcggttttga caccgaagtt ggaacaaaac cggaacattt
     3481 tcttggtgag aaaaggaaga ggattaggat tgttgggtga aaacaaatcc ttactggagg
     3541 cggcgagcgg attcgaaccg ctgcatcgag gttttgcaga cctctccctt aaccacttgg
     3601 gtacgccgcc ttgaagcgat agctaacatg atctttctct tacatgcaag aacgtcgcac
     3661 cttatgttga caaccatagg ctaaccacgt aattaaaaag caaatcggag aattttttaa
     3721 tggcaacgat gattaccagc gaatgtatta attgcggggc atgcgaacct gagtgtccaa
     3781 acaacgcgat cagtcagggt gatccgattt atgtcattga cccacttcta tgcactgagt
     3841 gcgtcgggtt tcatgatttc gaagcctgcg cggctgtctg tccggttgat tgctgtgtaa
     3901 ccgaccccaa caatatagag agcgaagaag ttctgattgg ccgggccaaa gccctccacc
     3961 ccgaaaccag ctttgacgat gacttccagt cccgatttcg caaagctaac ggagcgcaga
     4021 tcgatgtcac cattagtgca tcggagagaa atgaggtacc ccct
//
LOCUS       DASUTG010000630         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_393625, whole genome shotgun sequence.
ACCESSION   DASUTG010000630 DASUTG010000000
VERSION     DASUTG010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393625"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..1839
                     /locus_tag="VF089_14525"
     CDS             565..1839
                     /locus_tag="VF089_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6175222.1"
                     /translation="MALVKLEKTLAPVYSPAGRPLRIAQIAPLYESVPPKLYGGTERI
                     VAYLVEELARRGHQVTLFAAGDSTVNVPLWAGVPKSLRLSGLDHLGAVFHLPMLSDVY
                     DQAARFDIFHSHVDCLSFPLARFSQVPTLTTMHGRLDLKELLPIYRYYKDQPVISISH
                     DQRRPLPEMNWVGTVYHGLPRNLLKFRSEPGDYLAFLGRLSPEKRPDLAIEIARRSGI
                     PLKIAAKIDRADREYFDTVIRPLLSTPGVEFIGEINEAQKQEFLGGALALLFPVDWPE
                     PFGLVMIEALACGTPVIARPCGSVPEVLRHGISGFIESSIDDLVQAVHKVRDIPRRTC
                     RDEFERRFTAEVMADNYEQVYYQLADVRKAQGTAVRPKRTIHDVALAREAARQWHRAP
                     EGEALNAHPKSEAPVLKLFSRREERDPTNLNS"
BASE COUNT          545 a          634 c          650 g          518 t
ORIGIN      
        1 cgccatcgag ctattgaaat ggactgaaat ggtcggcagc gcaacgatga tcgaacgcga
       61 gtcgagggcg gtcatgaact gtccgagaca gacattgatc agcgcgagcc gctgcaattt
      121 ggcgggaaga tgtgtcgccg acatggagat gtaaatttga cggattaact gaatgtcgat
      181 gggtaatcgc gcgctaattc tctggaggcg gaggattcaa ggacacgagc gctgttaccc
      241 gtatggaagg cgatcagaac caaatctcct gtttggtaag ttattgctgt caagagcaat
      301 aacttacgtg ggttgatcaa atatcgcaac gcaaggttct gctagggatt ggccgcgacg
      361 gcatctcggt caatggcgaa gtagcctgag gttggagtcc tttttaacga ccggagcatt
      421 tcgcggctca gcgaattgat caacgtgctg gacgttgatc catgaaagtt cccacgttag
      481 ccccttgcca accggaattt gcggattata tttactccgt ctcggctcag agaccacaaa
      541 acacgcgttt tttcagaggt gaacatggca ttggtaaaac ttgagaagac gctcgcccca
      601 gtttattctc cagcaggcag acctcttcgt atcgcgcaga tagccccctt gtatgagagc
      661 gtgccgccca aactttatgg cggtacggaa cgcattgtcg cctatctggt ggaagagctt
      721 gctcgccggg gacatcaagt taccctgttt gctgccggcg attcgaccgt caacgtgccc
      781 ctatgggctg gagttccaaa atcacttaga ctcagcggac tcgatcatct cggcgcggtg
      841 ttccatctac cgatgctaag cgatgtgtac gatcaagccg cccggttcga catatttcat
      901 tcgcatgtcg actgcctcag ttttcctctg gcgcgatttt cccaagtacc gacactaaca
      961 acgatgcatg gccggctgga tctcaaggag cttttaccga tctaccgtta ttataaagat
     1021 cagccggtta tctcgatcag ccacgatcag cgccggcctc tcccagagat gaattgggtc
     1081 ggcaccgtct accatggctt gccacgcaat ctgctaaagt tccggtccga accaggcgac
     1141 tatttggctt ttctggggag gctgtctccg gagaagcggc cggacctcgc catcgagatc
     1201 gcgcgtcgtt caggaatccc acttaagatc gcagcgaaga tcgaccgcgc cgatcgcgaa
     1261 tacttcgaca ctgttatcag gccgttgcta tccacccccg gtgtagagtt catcggcgaa
     1321 ataaacgagg cacagaagca ggaatttctt gggggagcgc tcgctttgct cttcccagtc
     1381 gattggcccg agccttttgg gctggtaatg atcgaggcac tcgcttgcgg tactcccgtc
     1441 attgccaggc catgtggctc tgttcctgag gttctccgtc acgggatcag cgggtttatc
     1501 gaatcttcta tcgatgattt ggttcaggca gtacacaagg tccgcgatat cccacgccgc
     1561 acatgtcgcg acgaattcga aaggcgcttt accgccgaag tgatggccga caactacgaa
     1621 caggtttact accaactggc cgacgtccgc aaagcgcaag gaaccgccgt acgacccaag
     1681 cgcacgatcc acgatgtggc gctcgcccga gaagccgccc gacagtggca ccgagcgccg
     1741 gaaggtgaag ctttgaacgc acacccgaaa agcgaggcgc cagttctcaa gctattttca
     1801 agacgcgagg agagggatcc aacgaatctc aatagctagt cgctggcaac ccaaaccttg
     1861 gagggaggaa catgtccaac tcggaagtgg ctcctaatgc aatcgagctg ctcgaatcct
     1921 tgacgcccta tgggccatac ctttcgtatc cgagcagcca ggccgggaaa tttgacccgt
     1981 ggaaattcac tgtgggagaa ctctatgggt gcgtctgtca ccgtcctttc gatgaccttg
     2041 caccaacgcc ggaccgctcg aatcctgata gtggcctagc agggtgctga aaaaagcctc
     2101 ttgcaggctg ctcaaaaaga tccagatgcg aggcgcgcga aaaatcgacg agcggaggcg
     2161 tacttacagt acgttggagc gaggcgattg agcggcaacg aagcataggg tctttttcag
     2221 caacctgcta gagctcacgg accaggcgct gaagatggcc ggctgggatc agcggtcgac
     2281 gtctgaggca tcgcatgaag gcgtcacttc gcatctaggg ttatctccgt atcgatcgca
     2341 gctatag
//
LOCUS       DASUTG010000631         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2024001, whole genome shotgun sequence.
ACCESSION   DASUTG010000631 DASUTG010000000
VERSION     DASUTG010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024001"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..889)
                     /locus_tag="VF089_14530"
     CDS             complement(359..889)
                     /locus_tag="VF089_14530"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6175223.1"
                     /translation="MGKKKSALTAAIIFGVGLAATPAFSQSAPGGKSVPPNLDRTPTT
                     PGREQQLPPSGREIPKGTEGMAASDIKQVQQALKAKGHDAGTSGVMDDKTREAIRAFQ
                     KKEGLTMTGTVDEKTAQALGVSSKSSSGSGASGSRGSSGATGSSGSSGSSGATGGGAS
                     GRSSGSSGSVDKSPSR"
     gene            1327..1971
                     /locus_tag="VF089_14535"
     CDS             1327..1971
                     /locus_tag="VF089_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX6175224.1"
                     /translation="MITSIIFDLDGLLADTERLHCMAYQDALRAQGVAVSDHEYAEHW
                     VRSGRGISEWISERGLTLDPLALRAHKSRCYLDLLLSELRPMEGALALLEQLHHRKEL
                     ALASSSYPDAVEGVLAGLGIAHYFQVIVTGVDVPRLKPAPDIFLAAAQQLGAPPSQCL
                     VLEDAEKGVIAASLAGMRCIAVPNDHTRHHDFSKATRVCDSLNDVTLELLDTLE"
     gene            2028..>2637
                     /locus_tag="VF089_14540"
     CDS             2028..>2637
                     /locus_tag="VF089_14540"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX6175225.1"
                     /translation="MSRVIIIGAGIAGLAAAIGLRRLKIDTSIIEQATELAEVGSGIS
                     LWPNALKALASLGIDGAVRRLAIEEGSGGMRSWSGRLLFQLDLQELRRTLGDFTVVLH
                     RAELLSTLRDLAAAADIRLGARCLGFEQNDQRVKALLSDGTSVEGDLLLGADGLHSVV
                     RAQLFGANKPRYAGYAAWRGVTQFENNRILPGISLGRGSQFGQ"
BASE COUNT          563 a          722 c          727 g          625 t
ORIGIN      
        1 gtcgccctca aaaaatttcc actgcagctc agtggccagc cgcctgccga tggtagtttt
       61 gccggagccg gttacacccg tgatcacaat tatcatgtcg agaacaatcg tagcacttgg
      121 agatggaagg acacaattag acgatcggca tgacccacag gacaagttac acccgatcct
      181 cgatccgaga cttgcggcag tcggattttc cctgtcgcta attttatagg cgagatgata
      241 tgcggctcga tggaggcaga aaagatatca atgtgcagag gccggggttc ttacccggcc
      301 tctgcgtgta aagcggagtg cgcgaacgca acgttcaaca agctagtgat taccggtgtt
      361 accggctcgg cgatttatcc acggaaccgg aagagccgga cgatctccct gacgctcccc
      421 caccggttgc tcctgacgaa ccggatgaac ccgatgaacc cgtcgctccc gatgagccgc
      481 gcgacccgct cgcacctgac ccgctggaag acttactgct cacgcccaaa gcctgggcgg
      541 tcttttcatc aacagttcca gtcatggtca agccttcttt cttttgaaac gcgcgaatag
      601 cttcacgagt tttatcatcc atcacacccg atgttcccgc atcgtgacct ttcgccttca
      661 gggcctgctg tacctgcttg atgtctgaag ccgccattcc ttccgtgccc ttcgggatct
      721 cccttccgct gggaggtaat tgctgttcgc ggcctggtgt ggtcggagtg cggtcaaggt
      781 tagggggtac gctttttccc cctggcgcgc tctgcgagaa agccggcgtc gcagccaggc
      841 caactccaaa aattatcgct gctgtcaaag ctgatttttt ctttcccata gttacctcct
      901 ttgaactaac gattaaacaa cgttgcgccc agctgtgaaa acgcaaccgc tatgccactg
      961 acggatgaac acaatttcga gaaaatgcct cgattaaagt aactcccctg tttcggcgat
     1021 ttatcactga ttgttccgcc ttattagctc ttcggcgcat ctggcgtgtt aaacctgaga
     1081 cacagttttc catggatttc attttcgaga cacccacatt gactcatgtc ctttgatcca
     1141 aacatgattc aagattagcc tgtcgcgtat atcatgcacc cgggctgaag ggggattcat
     1201 ccggctaccg tcgaacggat tttgtaaata gcgggtccag ctacatggac gagcggtgaa
     1261 gcttcaaacg atcgcgtatg cctgataatc taaaatgcac atctgcaggt tggcgtgctc
     1321 gtagcaatga taaccagtat tatttttgat ttggatggcc tcttggctga taccgaacgc
     1381 ttgcactgta tggcatacca ggatgcgctc cgcgctcagg gcgtggccgt cagcgaccat
     1441 gaatatgccg agcattgggt tcgcagcggt agaggaatca gcgagtggat tagcgagcgc
     1501 ggattaactc tggatccact tgcgctgcgc gcccacaagt ctcgctgcta tctggatctg
     1561 ctactatcgg agctgcgccc gatggaaggc gcgctagctc ttttggagca attgcatcac
     1621 cgaaaggaac tcgcgcttgc gtcatcgtca tatcccgatg cggtagaggg tgttttagcg
     1681 gggctcggca tcgctcatta cttccaggtg atcgtcaccg gtgtggatgt gccacggctc
     1741 aaaccagcgc cggatatatt tctcgcagcg gcccaacaac ttggcgcgcc accgtcgcaa
     1801 tgcctagttt tggaagatgc cgaaaaggga gtgatcgcgg caagccttgc cggaatgcgc
     1861 tgcatcgccg taccgaacga ccatacccgc catcacgact tctcaaaagc gactcgagtc
     1921 tgcgattctc tcaatgacgt tacgttggaa ttgctggata cattggagta actcgatggc
     1981 ggcacgggat gcagagagac tcacattatt taatcaaggc taacattgtg tccagagtca
     2041 tcatcatcgg cgcgggcatt gcgggattgg cagccgctat aggacttcgg cggctaaaga
     2101 tcgacacttc gataatcgag caggcgacgg agcttgccga ggtgggttcc ggtatctccc
     2161 tatggcccaa tgccttgaaa gcgttggcga gcctgggaat tgacggcgcg gttcggcgac
     2221 tcgccataga agaaggtagc ggcgggatgc ggtcttggag cggcaggctc ctgtttcaac
     2281 tagaccttca ggagcttcgg cgtactctcg gagatttcac ggtagtactg caccgagccg
     2341 agttgctttc gacgttgcgc gatctggcag cggctgccga catacgcctg ggcgcccgct
     2401 gtctaggttt cgagcagaat gatcagcggg tgaaggcttt gctgtcggat ggcacttcgg
     2461 tcgaaggtga tcttctgctt ggcgctgacg gtctccattc ggttgttcgt gcgcagctct
     2521 tcggcgcgaa caagccgaga tacgcaggct acgccgcctg gaggggcgtc actcagttcg
     2581 aaaataatcg aatactgcct gggatttccc tgggccgtgg cagtcaattc ggccagg
//
LOCUS       DASUTG010000632         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1578998, whole genome shotgun sequence.
ACCESSION   DASUTG010000632 DASUTG010000000
VERSION     DASUTG010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578998"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VF089_14545"
     CDS             complement(<1..167)
                     /locus_tag="VF089_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX6175226.1"
                     /translation="MKDVLIYDTTLRDGCQAEDISFTLEDKLRIAEKLEELGVDYIEG
                     GYPGSNSRDAD"
     gene            complement(164..1387)
                     /locus_tag="VF089_14550"
     CDS             complement(164..1387)
                     /locus_tag="VF089_14550"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6175227.1"
                     /translation="MPLLVQKYGGSSVATIERIKHVAEKVCAAQRAGHDLVVVVSAMA
                     GETNRLLELAHEISEMPDEREKDVLLASGEQVSIALLSLALKERGQPSRSFLGHQVRI
                     ETDNAYGKARIRNIDSKRIMQSLKAGEVVVVAGFQGIDEDDNITTLGRGGSDTTAVAL
                     AAFLNASACEIYTDVEGVFTTDPGICADAQKLLRVSYEEMIELASTGAKVLEIRSVEF
                     AKRFAVPVHVRSSFSDVEGTWVVKEDESMEDLLVSGVSYDKNEVKITILRVPDRPGLA
                     AQVFGPIADAHIVVDMIIQNASADGTTDLTFTVSKADYKKALSIVEKTAPAIAAKGVD
                     VDTDIAKVSVVGVGMRTHAGVAAKMFRVLAEEGVNIQMISTSEIKISVVIAAKYTELA
                     VRVLHEAFIGKQGNV"
     gene            complement(1460..1945)
                     /gene="tsaE"
                     /locus_tag="VF089_14555"
     CDS             complement(1460..1945)
                     /gene="tsaE"
                     /locus_tag="VF089_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6175228.1"
                     /translation="MDWVVSSRSAEHTRRLGARLGKLLQGGEIIGLVGELGTGKTCFV
                     RGLTEGLEVSRETWIRSPTFTLVNEYQGRLPIYHIDLYRIESTGELEELNLREYLYSN
                     GVSLIEWFQYFPAGEIEDHLEVELAYREGSKRQLTFLPHGARYEDLVKALQQQLSINE
                     R"
     gene            complement(1999..3552)
                     /locus_tag="VF089_14560"
     CDS             complement(1999..3552)
                     /locus_tag="VF089_14560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6175229.1"
                     /translation="MILVTAEQMREMDRLTIQKYGTPSLTLMERAGTGVVTAMLERFA
                     EPASKGVLVVAGKGNNGGDGFVVARLLREQGVGCEVALLAKPDEISSDAAHNLEAYSR
                     AGGKVVDIAADRLALLADSIAGKGLLVDAILGTGTKSEIRGIYADAIRAMNQSGLPIV
                     AVDIPSGLDADKGTPLGVCIQAAMTIALGYPKLGQVIHPGVEYVGHLVVADIGIEPAA
                     VSEVAPSGQLLTLEQVRPLVPVRKPDSHKGTYGHVLVIAGSRGKTGAAILASRACMRA
                     GAGLTTLAAPRSLNNIFAGAMIEVMTEPLRETTDEEIECLTDDEWRGLLERKTVALFG
                     PGIGVTDCTRNNLRWLLRHLDVPWVIDADGLNNLALDIERLRKARTAPILTPHPGEMA
                     RLTGKDVRELNKDRIGVARAFATEHQCYVVLKGARTVLATPAGKIFINPTGNPGMASG
                     GMGDVLAGILAALLGQGLSPEAAMKLAVYLHGFVADRISAERGMLGMIASDIIDGLPT
                     GYRSLTAAL"
     gene            complement(3635..>3745)
                     /locus_tag="VF089_14565"
     CDS             complement(3635..>3745)
                     /locus_tag="VF089_14565"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX6175230.1"
                     /translation="ELNIGHSIIARAIIVGIEQATREMKDILNKSRKQPE"
BASE COUNT          807 a         1143 c          939 g          856 t
ORIGIN      
        1 aaatccgcat cccggctatt cgatccggga tagccgcctt cgatgtagtc tactcccaac
       61 tcttcgagct tctcggcgat gcggagcttg tcctccaagg taaatgagat gtcctccgct
      121 tgacacccat cccgtagtgt cgtgtcgtag atcaaaacat ctttcataca ttcccctgtt
      181 tgccgatgaa cgcctcgtgc agcacccgca ccgccagttc cgtgtacttg gccgcgatga
      241 cgaccgagat cttgatttcc gaagtggaaa tcatttggat gttgactccc tcctcggcca
      301 gaactcggaa catctttgcc gccactccag cgtgtgtgcg cataccgaca ccaacgacgg
      361 agactttggc gatatcggta tcgacatcaa cccctttcgc cgctatagca ggcgcggtct
      421 tctcaacgat ggataaagcc tttttataat ccgctttaga aaccgtaaaa gtcaaatctg
      481 tagtgccgtc cgcgctggca ttttgaatga tcatgtcgac gacgatgtgg gcgtcggcga
      541 tggggccgaa cacctgggcg gcaaggccgg gccggtcggg tacacgaagg atggtgattt
      601 ttacttcgtt cttatcgtag ctcacgccgg agaccagcag atcttccatg ctctcatcct
      661 ccttgactac ccacgtccct tcgacatcgc taaagctaga acggacgtgc actggaacag
      721 cgaatctttt ggcgaactcg accgaacgga tctccaggac tttagcgccg gtgctggcta
      781 gctcgatcat ttcctcgtag gaaactcgca gcaatttttg cgcatcggcg caaattccag
      841 gatccgtggt gaaaaccccc tcgacatctg tatagatttc gcacgcgctg gcattgagaa
      901 acgccgccaa agccaccgcg gtggtgtccg atccgccgcg acccagggtg gtgatattgt
      961 cgtcctcatc gattccctga aagcccgcca cgaccacaac ttcgcctgcc ttcaatgatt
     1021 gcatgattct cttggaatcg atgttgcgaa tgcgcgcctt gccgtatgcg ttgtccgttt
     1081 ctattctcac ctgatgaccg agaaaggagc gcgacggctg cccacgctct ttcaacgcga
     1141 ggcttagcaa cgcaatcgac acctgctcgc ccgaagccag caaaacatct ttttcacgtt
     1201 cgtcgggcat ctctgaaatc tcatgcgcaa gttccaaaag ccggttggtt tctccggcca
     1261 tagccgagac gaccacgact aggtcatgac cggctcgctg ggctgcgcaa accttttcgg
     1321 ccacatgctt gatgcgctct atcgttgcga cggaagaacc gccgtatttc tgaaccagca
     1381 atggcatatt gatgcgcgac aagcccttca acagtttaga ggtactgcga aaacccccaa
     1441 cagttcgccc gccgaacctt tacctttcgt taatactcaa ttgttgctgt aatgccttga
     1501 ccaaatcctc gtagcgcgcc ccatgaggta aaaacgtcag ctgacgtttg ctgccttctc
     1561 gatacgccag ctcaacttcg agatgatctt cgatctcgcc ggcaggaaaa tactggaacc
     1621 actcaatcag gctgacgccg ttggagtaga gatactctcg gagatttagc tcttcgagct
     1681 cgcccgtgct ttcgatccgg taaagatcga tgtgatagat gggcaagcgg ccttgatact
     1741 cattgacgag tgtaaacgta ggactgcgaa tccaagtctc tcggctgacc tccaacccct
     1801 ccgtcaaccc tctcacaaag caggtcttgc ccgttcccaa ttccccaaca agaccaataa
     1861 tttcgccgcc ttgcaacagc ttacctaatc tagcgcccaa acgtcgggtg tgctcggcgg
     1921 agcgtgaact aaccacccaa tccatcagca acaccgctcc ataagcacaa ctagtatatt
     1981 ttgcaggaaa tttcgagtct aaagagcagc cgttagactg cgatacccgg tgggtaatcc
     2041 gtcaatgata tcggaagcaa tcatgccgag cattcctcgc tccgcagaaa tccgatcggc
     2101 gacaaaacca tgcagatata ccgccaactt catcgccgct tcaggagaga gtccttggcc
     2161 gagaagtgcc gctaatatgc ctgcgagaac gtctcccatg cctccgctcg ccatccctgg
     2221 attccctgtc ggattgatga aaattttgcc cgccggtgtg gcgagcactg tgcgcgcgcc
     2281 tttaagcacc acatagcatt gatgttctgt cgcaaacgcc cgcgccacgc cgatgcggtc
     2341 cttgttgagc tcccgtacgt ctttgccggt gagccgcgcc atctcacccg gatgcggcgt
     2401 caagatcggc gcagtcctgg ccttgcgcag ccgctcgata tcaagggcga ggttattcag
     2461 cccgtcggcg tcgatcaccc aggggacatc caaatggcgc aacagccaac gcaggttgtt
     2521 tcgcgtacag tcggtgacac cgattcccgg accaaacagc gccaccgtct tgcgttccaa
     2581 cagccctcgc cactcgtcat ccgtcagaca ctcgatttct tcgtcggtag tctcgcgcag
     2641 cggttcggtc atcacctcga tcatggctcc ggcaaagatg ttgttaagcg atcgcggcgc
     2701 cgccaaagta gtcagtccgg cgccggcgcg catgcatgcg cggctggcaa gaattgccgc
     2761 gcccgttttg ccccgggaac cggcgatcac taatacatgt ccgtaggttc ccttgtgcga
     2821 atccggtttg cggaccggaa ctaatggacg tacttgttcc agagtcagta gttgtccact
     2881 cggcgccact tcgcttacgg ccgccggctc aatgccgatg tccgcaacca caagatggcc
     2941 gacgtattcc acgcctggat ggatcacttg gccgagcttg ggataaccca gcgcgatggt
     3001 catggcagct tgaatgcaca cccccagcgg tgttcccttg tcggcatcca gcccggaagg
     3061 tatatcaaca gcgacaatgg gcagcccaga ttggttcatt gcgcgaatag cgtcggcgta
     3121 gatgccgcgg atctcacttt ttgtgccggt acctagaatg gcatcgacca gcagtccttt
     3181 gccggcgatt gaatcggcga gcaatgctag acgatcggcg gcgatgtcga ccaccttgcc
     3241 tccggctctc gaataggcct ccaggttgtg ggctgcgtcg ctggaaatct cgtcgggttt
     3301 ggccaacaag gcgacttcgc agcctacccc ctgctctcgg agcaagcggg ccaccacaaa
     3361 accgtcgccg ccattgtttc ctttaccggc aacgaccaag acacctttgc tcgcgggctc
     3421 ggcaaaacgc tctagcatgg ccgtcacgac tcccgttcct gcgcgctcca tcagcgtaag
     3481 actcggagtc ccatacttct ggatcgttaa tcgatccatc tcgcgcatct gctcggcggt
     3541 aaccaggatc attattccga aagctcaagt cgtgcgaaac gctcaagcca tgccctactc
     3601 agcgaatcgg attaactctt agctgttaaa ttgactactc aggttgtttc cgtgatttgt
     3661 tgagaatgtc cttcatctca cgagtggctt gctcgatgcc gacgataatc gctcgagcga
     3721 taatactgtg accgatgttg agctc
//
LOCUS       DASUTG010000633         4134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2024368, whole genome shotgun sequence.
ACCESSION   DASUTG010000633 DASUTG010000000
VERSION     DASUTG010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024368"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1717)
                     /locus_tag="VF089_14570"
     CDS             complement(131..1717)
                     /locus_tag="VF089_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6175231.1"
                     /translation="MEAILIDSEWDVILIGAGQNNFALGTYLGLAGLRTVICETRLEN
                     GGRLASEEITIPGYWHNTLAYFQDNREISPVWKELKWDNGYHAEFVSPPVISSLLLAN
                     GRSVSHHQTLQDTLSSIARLSSKDADAWRRTHDNFHPVIRECLIPYYYRAPIPDNALD
                     ARLETEPHGQEFRRLWRMTPRQAVDELFENEAVKALVLSQMAIPRGIAVDYQGGAIEV
                     LKLIAGDEKPELARGGSHSIAQVLQRAYVHAGGQIRAVHHVDKILVEKDKAVGVRLRD
                     GREWKAKLAIVSNTDPYATFKELIDQEHLPRAFSERIEAIEADEFSYFQVHLALKAPV
                     RYALHEADEPGVARAMNVNIGPEKAEDVSEMWKEIRAGDFPQHVCLHAICPTVFDPLQ
                     APRGKHTASLFMPVPYQLKGKRPEDWVKLKDGFMNDLLCIWRRYATNLTDDNIEMKVA
                     MDPFYIAGRWPNMRRGSVWVARKTAAQMGANRPNERLSDYRTPIAGLYQVGVGTHPAD
                     AVIAGSGYNCWRIMKEDLKL"
     gene            complement(1718..2737)
                     /locus_tag="VF089_14575"
     CDS             complement(1718..2737)
                     /locus_tag="VF089_14575"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175232.1"
                     /translation="MPTVRILSIIWLVVMIVLPGAIYGQNAQLVEGAKKEGGKVVVYG
                     SIENDTMDLIAAALKKKTGLEIEFWRSSSTKVMDRVLNEMRTGKPLYDVVLTNTAPME
                     FMFNEGALAKYDSPSAKAFPKEVMHPNLGPQYRNSVIGIVYHAGIIKPAEAPKSLEDL
                     LKPQYAGKLVIPDASQHTTTAQWMASLHKVLGGKEKADKFIRDLAATKPLLVESLTPA
                     GERITTGERPIGIAFLKNVVFYGKKGVPLDYVRLGKFMGDGQAVAIGAKPAHSNAGKA
                     FIDFFIGEEGLKLMAGIGEFVTRKGIYPPLPDADKIELVEMIDMDKQAYAEKMAEYRK
                     IFLQN"
     gene            2842..3960
                     /locus_tag="VF089_14580"
     CDS             2842..3960
                     /locus_tag="VF089_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6175233.1"
                     /translation="MTGQEKLKKIRDELRQTFLERADLIDGALAALLSSHHILIIGPP
                     GTAKSMLADELCRRIEGGKYFQWLLTRFTTPEEIFGAVSLKALEQDDYRRVTSDKLPE
                     AQIAFLDEIFKANSSILNSILTLLNERLFHNGKTVTRVPLLTLFGASNELPEEEELTA
                     LYDRFLLRFVVGYIAEDFRFLRMLESQKQPERTTLSLAELEDLQNQVKAVSIPSHVYR
                     GIADIRRELNKKNIVASDRRYRQSLALLQAHAFLNGENEVAEKDLFFLEHVLWRDPAE
                     HEQVRATIRDLILGYEEEISELLYESREIRDSATRPWATSDEKARALIEFHTKLRNIL
                     AKVDQIVDKAKRLGRPVERVNTVRNEIEQLQKQMLEQF"
BASE COUNT          904 a         1167 c         1080 g          983 t
ORIGIN      
        1 aagaagaaaa acccatggcc ggatcattcg agtaatttga agaatcgacg ttatgaacag
       61 tttgaacatc cgaggatcgt taacggcttg aactacttga acagcttaac cgttttgaac
      121 aaatgatttc ttaaagtttt aaatcttctt tcataattcg ccagcagtta tacccggagc
      181 ctgcgatcac cgcatcggcc ggatgggtcc cgaccccgac ctggtacaga cctgcaattg
      241 gcgtgcggta atcagacagg cgctcgttgg gccggtttgc tcccatttgg gctgcggttt
      301 tgcgcgccac ccataccgag ccccggcgca tgttgggcca ccgaccggcg atataaaatg
      361 gatccatggc aactttcatt tcgatattgt catctgtcaa atttgtcgcg taacggcgcc
      421 atatacatag aagatcattc atgaagccgt ccttgagctt gacccaatcc tccgggcgtt
      481 tgcctttcaa ctgataaggc acgggcatga acagcgatgc cgtatgcttc cccctcggcg
      541 cctgcagcgg atcaaaaacc gtcgggcaaa tcgcgtggag acacacatgc tgtggaaagt
      601 cccccgcgcg aatctctttc cacatttcac ttacgtcctc agctttctcc ggtccaatgt
      661 tgacgttcat ggcccgagcc acgcctggct cgtccgcttc atgcagcgca tagcgaaccg
      721 gcgccttcag agcgaggtga acctggaaat aggaaaactc atcggcttcg attgcttcga
      781 tccgttcgct gaatgcacgg gggagatgct cctgatcgat cagttccttg aacgtcgcat
      841 acggatcggt gttggaaacg atggcgagct ttgccttcca ttcgcgcccg tcgcgcaggc
      901 gtactcccac cgctttgtct ttttcgacga gaattttatc gacgtgatgc acggcgcgaa
      961 tttgaccgcc ggcatgaacg taagcccgtt gcagcacttg cgctatggaa tgggagccgc
     1021 cacgggccag ctcgggtttt tcatcgccgg caatcagttt aagaacttca atcgcgccgc
     1081 cctgataatc cacggcgatg ccccggggaa tcgccatctg cgacagcacg agggctttca
     1141 ctgcctcgtt ctcgaacaat tcgtcaaccg cttgacgggg agtcatgcgc caaagccgcc
     1201 tgaattcctg gccatgaggc tcggtttcta acctggcatc gagggcattg tccggaatcg
     1261 gcgcgcggta atagtaggga atcaaacatt cgcgaatcac aggatggaag ttgtcgtggg
     1321 tccgtcgcca tgcgtcggcg tctttcgatg ataaccgcgc aatcgagctc agcgtgtctt
     1381 gtaatgtttg atggtgcgaa accgagcgac cgttggcaag cagcaacgag cttattaccg
     1441 gcggggaaac gaattcggcg tgatagccgt tgtcccattt gagttctttc caaactgggg
     1501 agatttcgcg gttatcctgg aaataagcca gggtattgtg ccagtaaccc ggaatcgtga
     1561 tttcttcgct ggccaaccgt ccgccgtttt ccaggcgagt ctcacaaatc accgtgcgca
     1621 gccccgctag cccgaggtag gttccgagag caaagttgtt ttgcccggcg ccgattaaaa
     1681 ttacgtccca ctcactgtcg atcaaaatcg cctccatcta attctgcaga aagatctttc
     1741 gatactccgc cattttttcg gcgtacgctt gtttatccat gtcgatcatc tcgaccagct
     1801 cgatcttatc ggcgtccggc aggggcggat agatcccctt gcgggtgaca aattcgccga
     1861 ttcccgccat caacttcagc ccttcctcgc cgataaagaa atcgatgaac gccttgccgg
     1921 cattggaatg ggccggcttg gcgccgatgg ctaccgcctg tccatcgccc ataaatttgc
     1981 ccagccgcac ataatccagc ggcactccct ttttaccata gaacaccacg tttttaagaa
     2041 aagcgatgcc gataggtctt tccccggtgg taattcgctc tcccgccgga gtcaaagatt
     2101 ccaccagcaa gggttttgtc gccgctagat cgcggataaa tttgtcggct ttttctttgc
     2161 cgcccaatac tttgtgcagg ctcgccatcc attgcgccgt ggtcgtatgc tggctggcat
     2221 ccgggattac cagctttcct gcatactgcg gcttgagtag atcttccagc gactttggcg
     2281 cctccgcggg tttgatgatt ccggcgtggt aaacaatgcc aatgacgctg ttgcgatact
     2341 gcggcccgag attagggtgc atcacttctt tgggaaatgc tttcgccgag ggcgagtcgt
     2401 atttcgccag cgcgccttcg ttgaacatga actccatcgg cgcggtgttg gtcagcacaa
     2461 catcgtagag cggcttgccg gtgcgcatct cgttgagcac gcgatccatg accttggtgc
     2521 tggacgaccg ccagaattct atttccagcc cggtcttctt cttgagcgcc gcggcgatga
     2581 gatccatggt gtcgttctcg atggagccgt agacgacaac cttgccgccc tcctttttgg
     2641 cgccttcgac cagctgagca ttctgaccat agatcgcgcc cggcagaacg atcatcacga
     2701 ctagccagat gatagataaa attcttacgg tcggcatagg cgagccctcc tcccttcgtg
     2761 cactgctccc tcattatata aatgatcggc ggatgtctat ttgtttctac cagggccatt
     2821 tggtaacttt atcgaaaacg tatgactgga caggaaaagc tcaaaaagat ccgtgatgag
     2881 ctgcggcaga cctttctcga gagggccgat ttgattgacg gcgcgcttgc cgcgctgctg
     2941 tcctcgcatc atatactcat catcggaccg ccgggcacgg ccaagtccat gctcgccgat
     3001 gaattatgcc gccgcatcga aggcggcaag tattttcaat ggctgcttac ccgttttacc
     3061 accccggagg agatcttcgg ggcggtcagt ttaaaggcgc tggaacagga tgattatcgg
     3121 cgcgtcacca gtgataaact gcccgaagcc cagatcgcct ttctcgatga gatcttcaaa
     3181 gccaattcat cgatcctgaa ctccattctc acgttgctga acgagcggct tttccataat
     3241 ggcaaaaccg tcacccgggt gccattgctg accttgttcg gtgccagcaa tgagctgccc
     3301 gaggaagaag agctcaccgc gctctacgat cgctttttgc tgcgctttgt ggtcggttac
     3361 atcgccgagg attttcgctt cttgcgcatg ttggaatctc agaaacaacc ggagcggaca
     3421 acgctgagtc tagccgagct ggaggatctg cagaatcagg tgaaggcggt atcgatccca
     3481 tcgcacgttt atcgcggaat tgccgatatc cggcgcgagt taaacaagaa aaatattgtc
     3541 gcttctgacc gccgttatcg tcagtctctg gccttgcttc aggcgcatgc atttttaaac
     3601 ggcgaaaatg aagtcgcgga aaaggatctt ttcttcctcg agcatgttct atggcgcgac
     3661 cccgccgagc acgagcaggt gcgcgccacg atacgcgatc tgatcctcgg gtacgaggaa
     3721 gaaatctccg aactgcttta cgaatcgcgc gagattcgcg attcggcgac tcgcccatgg
     3781 gccaccagcg acgagaaggc gcgcgcgctc atcgaatttc acaccaagct gcgcaacatt
     3841 ctcgctaagg ttgatcagat cgtcgacaag gccaagcgcc tggggcgtcc ggtggaacga
     3901 gtcaataccg tgcgaaatga aatcgagcag ttacaaaagc agatgctgga gcagttctga
     3961 tgcccgcgcg aagggcaaaa aaaacagcga tcaaacgggt ttttcaacct ctgccgcaaa
     4021 atactttctg gattgacagc gatgcgtacg accggcgcgc ttacggccag cttcgttcag
     4081 attccccgtc cctgcaaatg ttagaagaac aagggagcac tctcctgccc cact
//
LOCUS       DASUTG010000634         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_246436, whole genome shotgun sequence.
ACCESSION   DASUTG010000634 DASUTG010000000
VERSION     DASUTG010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246436"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VF089_14585"
     CDS             complement(<1..761)
                     /locus_tag="VF089_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175234.1"
                     /translation="MSTANMSYSEQQRSLADLMLAVAGESVTVLKKHAPEQALKLKRQ
                     DEWKLYLEFLKVTFNLADRLSAFHIPVKDQPDFMSGLEETVTARLRAVLEPAFGSGVD
                     QNEIMMTISGVVAESRKLYESYRFSITEDSKTKTEMFDSFGGRIADALGVADNPQITS
                     AALLCATAMIPAITAILKGEPPPLAGTSSQLSESAASTVGPAQRGQTGNEIKLISVMS
                     DVSGDEIETRWGLHPRFRQDLTAGEIQQLTKLMNRV"
     gene            971..1816
                     /locus_tag="VF089_14590"
     CDS             971..1816
                     /locus_tag="VF089_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEX6175235.1"
                     /translation="MESLPLLFVKTVVLRPYVFVFLAAFLLVGERLLGWRRTAVFFLT
                     SWITAFVCEYSSTRVGVPFGFYYYTGSTVGQELYIADVPFMDSLSFTFLLFSSYCLAL
                     VFLLPSVARAGQRRLQFVLDPKIRTSWSSLLLTATFLTVIDVIIDPVALRGDRWFLGR
                     IYGYPDPGVYFGIPLMNFVGWAVVGVVGLGMYFFVDRRLLPLTLHRDQSSATTPLLLG
                     CALYYGVLLFNLVITFWIGEPLLGLVGMMIYLPITIVLVLRLLNRIPTPRPAVGTTSA
                     LAPEA"
     gene            1900..>2660
                     /locus_tag="VF089_14595"
     CDS             1900..>2660
                     /locus_tag="VF089_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6175236.1"
                     /translation="MSRDITPYILKRQFEDAAPRTLSIDYAAALNPQQLAAVTAGDGP
                     SLVIAGAGSGKTRTLVYRVAYLIDSGVDPSNILLLTFTRKSAQEMLQRAGELIGTRSE
                     RVCGGTFHSVANMLLRRYGRGVGLEPGFTILDRSDAEDLIALVRSQLGLNEKDKRFPR
                     KSTIAEIFSKSENTLRSLDDILMEEFSHFADHLDALGQLQTGYRAAKRQRQLVDYDDL
                     LVLLRQLLMQDMTARQSIARLYRYILVDEYQDTNRL"
BASE COUNT          511 a          741 c          729 g          679 t
ORIGIN      
        1 actcgattca taagttttgt gagttgttga atctcgcctg cggtaagatc ctggcgaaac
       61 cgcggatgca atccccatcg ggtttcgatt tcgtctccgg acacatcgga catgacactg
      121 atgagcttga tctcattgcc cgtctgacct ctctgcgctg ggccgacggt cgaggccgca
      181 ctctcgctta gctgggatga cgtgccggct aagggcggtg gttccccttt aaggatcgcc
      241 gtgatcgcgg gaatcatcgc cgttgcacac agcaaagcgg ccgatgtgat ttgcggatta
      301 tctgccactc ccagcgcgtc cgcgatacgc ccgccaaagc tgtcgaacat ctcagtcttg
      361 gtcttgctgt cttcagtgat ggagaagcgg tagctctcat agagcttgcg actttccgcg
      421 acaacgccgc tgatcgtcat catgatctcg ttctgatcga cgcccgaccc gaaagccggc
      481 tccagaacag cgcgcaaccg cgccgtcaca gtctcttcca gtccgctcat gaaatccggc
      541 tggtctttga ccggaatgtg aaacgccgac agccggtcgg ccaggttgaa cgtcaccttg
      601 aggaactcga gatacaattt ccattcatct tgccgtttga gtttgagcgc ctgttcggga
      661 gcatgttttt tcaatacagt tactgattcg ccggcaaccg cgagcatcag gtcggcgagc
      721 gagcgttgct gttcgctata agacatgtta gccgtggaca tgatgacgtg tgctcctgag
      781 cgttgacaat ggggcattat aactgagtgg gaatcttccc tccaaggggt tcaagctgac
      841 caaagagctc ggtgagcgga gatcgttgcg gacgcgtcat cgtgtggttg ctgatccctc
      901 ttggcaaata cttgcgagtc gtccacaatg cggcgcaaaa aggtgaaagg agccggcctc
      961 cgacatcgcg atggaaagtc ttccactcct ctttgttaag acagtcgtac tgcgcccgta
     1021 tgtattcgtg tttctcgccg cgttccttct cgtgggcgag cggctgctcg ggtggcggag
     1081 aaccgcagtc tttttcctaa ccagctggat caccgccttt gtgtgcgaat attcgtccac
     1141 acgcgttggc gttccgtttg ggttttatta ttacaccggc tcgaccgtcg gccaggagct
     1201 ctacattgcg gacgtgccgt tcatggattc gctctcgttc acctttctgc tcttttcaag
     1261 ctattgtctc gccctggtat ttcttttgcc ttcggtagcc agggcgggcc agcggcggct
     1321 acagttcgtc ctcgatccaa agattagaac gtcatggtca tcgttgctac tgacggcgac
     1381 gtttctcacc gtcatcgacg tgatcattga tcccgtcgcg cttcgtggcg accgctggtt
     1441 tcttggacgc atctatggct atcctgaccc aggtgtgtat ttcggaatcc cgctcatgaa
     1501 tttcgtggga tgggctgtcg tcggggttgt cgggcttgga atgtactttt tcgttgatcg
     1561 gaggctcctt ccgctgacct tgcaccgcga ccaatcttct gcgaccacgc ctttattgct
     1621 tgggtgcgca ctgtattacg gagttctgct ctttaatctg gtcatcacat tctggatcgg
     1681 tgaaccgttg ctcggtctcg ttggaatgat gatctatctg cccattacca tcgtccttgt
     1741 tcttcgactc ctcaaccgga tccccacccc gcgccccgcg gttggaacca catccgcatt
     1801 ggcgccagag gcgtaaccga ccgcgctctc gatgtcttca tgtgccgcgt tgaaacgatc
     1861 tctttataat gttatacagc gagtcacttg tacttcctca tgagtcggga catcacgcca
     1921 tacattctga aacgccagtt cgaggatgcc gctcctcgca cgctatccat cgattacgcg
     1981 gcagccttga atccccagca actggcggcc gtcacggcag gcgatgggcc gtcgcttgtg
     2041 attgccggcg ccggaagcgg gaagactcgt acactcgtct atcgcgtggc ctatctgatt
     2101 gattcgggcg ttgatccttc caacattctt ctgctcacgt ttacgcgcaa gtcggcgcag
     2161 gaaatgttgc aacgagccgg cgagctgatt ggaacgcgaa gcgagcgggt gtgcggagga
     2221 acatttcact ctgtcgccaa tatgctgctg cggcgctatg gcaggggcgt cggcttggag
     2281 ccgggattca ccattttaga tcgaagtgat gccgaagatc tgattgcgct cgtgcggtcg
     2341 caactcgggc tcaatgaaaa agacaagcgt tttccgcgga agagtaccat cgcggaaatc
     2401 ttcagcaaat cagagaacac gctgcgcagc ttggatgaca ttcttatgga ggagtttagc
     2461 cactttgcgg atcatcttga tgcgctcggc caactccaga cgggttaccg agcagcgaaa
     2521 cgtcaacggc aattagtgga ctatgatgat ctccttgtac tgctccgcca gctgctcatg
     2581 caggatatga cggcgcggca atcgattgcg cggttgtatc gctacattct ggtggacgaa
     2641 tatcaagaca ccaataggct
//
LOCUS       DASUTG010000635         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2174490, whole genome shotgun sequence.
ACCESSION   DASUTG010000635 DASUTG010000000
VERSION     DASUTG010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174490"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF089_14600"
     CDS             <1..203
                     /locus_tag="VF089_14600"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX6175237.1"
                     /translation="NEVLRQRVSRIRNDDHYLEKLAREELNLVRPGEVIYRFPKYKPD
                     QDREDLLNDSPTAESRPSSARK"
     gene            complement(154..1017)
                     /locus_tag="VF089_14605"
     CDS             complement(154..1017)
                     /locus_tag="VF089_14605"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX6175238.1"
                     /translation="MSEKTFRKSIAHRYGISFPSILDLDDYCRLMKMQRLKTISSLRL
                     YCFGLLILPFTFLVLGAQDLAGGEDPETFFSEARRTMIERDIAARGIKDQRLLQVMGT
                     LPRHLFVPQEMRSFAYQDRPLPIGAGQTISQPYIVAFMTELLELEGRERVLEIGTGSG
                     YQTAVLAALAEQVFSVEILPDLSARAQKLLKQLSIQNIKLKVGNGFHGWAEEAPFDAI
                     MITAAAPKIPPPLWNQLSDGGVLIMPLGTAGRTQQLIRARKISGRQVIEEHSAVIFVP
                     MTGGTQQSGSR"
     gene            1280..1792
                     /locus_tag="VF089_14610"
     CDS             1280..1792
                     /locus_tag="VF089_14610"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6175239.1"
                     /translation="MEQMDPKRSALAIVAAIKKAGIDFVATLPDEKVLEVIRTVESDP
                     GLQHIPLCREEEGIGICAGAYLAGRKTALIMQNAGFLNSCNALTTTCLQFQIPVVLLI
                     YYAGDIGDRGFTTLGAVTEPVLQALGIRSYILRGTDEIDETIAGAEVLAEDSKRPVAV
                     LLTKSVLGVK"
     gene            1789..2391
                     /locus_tag="VF089_14615"
     CDS             1789..2391
                     /locus_tag="VF089_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6175240.1"
                     /translation="MMQRYDYLRAVAGDTGDALTVCTGWGAREWWAVRPSDGNLKTRT
                     LGLVSSIAAGLALALPHRKVIAIDGDGAFLMNLCGLPTIALQNPPNLIHLLFDNGVYE
                     ASGGTATASHIADAVSLAKGAGYPHACWVKNPEEFRARFLEALDGNRLILIAVKVDPG
                     QPKDLPPLKLDELENKYLFMRYLERTEKKAILSPGFDSKI"
     gene            complement(2416..3420)
                     /locus_tag="VF089_14620"
     CDS             complement(2416..3420)
                     /locus_tag="VF089_14620"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6175241.1"
                     /translation="MKRRRSKNRSAVSQFFYRLRYGLGAYVLRAFIFVLGRLPDRRVV
                     SAATFWAWISHKLLWRYRIRMEQNIATAAGDRFPNKAARKAMVWRVWKSFTRELFEAG
                     ALIDYPKEAIIAKIRVEGEENLRDALAQRKGVLALSAHLGNFALIGARLAAAGYPFSV
                     VVKHPSNEEFAQLITRYRAQVGIDTISAKPRREAVRAIVQALRENRIVLVIADEFKSG
                     EIMVEFMGLNLPAPRGPATLALRTGAVTLPMFATRQPDGSIVLAIGRAIEAVHRESLE
                     ETITATTALYTRHLEDVIRRYPDQWSWLGFPRKHRAVGSEMSNSRASKTEVSVTHRAL
                     "
     gene            complement(3539..>3832)
                     /locus_tag="VF089_14625"
     CDS             complement(3539..>3832)
                     /locus_tag="VF089_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175242.1"
                     /translation="QKYLLARDGKGRVLFLEAEEWILDEDTDWLFSFANVCEMLGFEP
                     SYLRDGLMRWKAEKLESHSKAKIYQIAARTTRRKSEVSVPRRSTQRMRKAASR"
BASE COUNT          894 a         1041 c         1013 g          884 t
ORIGIN      
        1 aaaacgaagt cttgcggcag cgggtctcgc gaatacgtaa cgacgaccat tatctggaaa
       61 aactagctcg cgaggaactg aacctggtgc ggcccggcga ggtcatctat cgttttccta
      121 agtataagcc tgatcaggac cgagaggatt tactcaacga ctccccgact gctgagtccc
      181 gcccgtcatc ggcacgaaaa taaccgcgct gtgctcttcg atcacttgtc gaccgctgat
      241 ctttctggcc cgaatcaact gttgagtacg ccctgcagta cctagaggca tgattaagac
      301 gccgccgtca ctgagctgat tccatagagg cgggggaatt ttcggtgccg ccgcagtaat
      361 catgatcgca tcgaacggtg cctcctcggc ccatccgtga aaaccatttc cgacttttaa
      421 ttttatattt tgaatactca gctgtttaag taacttctgg gcccgagcgc tcaagtcggg
      481 aagaatttct actgagaaga cttgttccgc taaggccgca agcacggccg tttgatagcc
      541 tgaaccggtg ccaatttcca gaactcgctc gcgtccttcg agttccaaca gctcggtcat
      601 gaatgctacg atgtacggct gggagatggt ctgtccggcg ccaatcggca gcggccggtc
      661 ttggtaggcg aaggagcgca tttcttgggg tacgaataag tggcgcggca gagttcccat
      721 gacctgaagc agccgctggt ctttgatgcc gcgtgcggcg atatcccgtt cgatcatggt
      781 ccgacgcgct tcgctaaaaa atgtttcggg gtcctcaccc cccgctaaat cctgtgctcc
      841 gagcactaaa aacgtgaatg ggaggatcag taacccgaag cagtacaggc ggagagaaga
      901 gatggtttta agcctttgca ttttcatcaa ccgacaatag tcatccaagt caaggattga
      961 gggaaaagaa atcccatatc gatgcgcaat cgatttgcga aaggtcttct cactcattct
     1021 gagatcccca acaagacagg tgcttgagtc aaccgtgaca tccctgattg cagccttcaa
     1081 tgtttaaaca ggcattttca tcttaggcac tgctccgttt gtgtcaagca tgcgtgcggc
     1141 aactcgcccg gtaacttgac aaggttaagg acacgcagta tttcgtcccg gtgtggccca
     1201 gctgtcgaga gcaaagggta tatatgatcg aggaccggcc gcaccttact ctctcttata
     1261 atcaagaggt ttcattttaa tggaacagat ggatcccaaa cgatctgcgc tggcaatcgt
     1321 tgctgcaatc aagaaagccg gcatcgattt cgtcgccacc ttgccggatg aaaaggtgct
     1381 cgaagtgatt cggaccgtcg aaagcgatcc aggccttcag cacattccac tgtgtcggga
     1441 ggaagaggga attggtattt gtgccggcgc ttatctggcc gggcggaaaa ccgctctgat
     1501 tatgcagaat gccggcttct tgaacagttg caacgcgttg accaccactt gtttgcagtt
     1561 tcagataccg gttgtgctgc tgatttacta tgccggggat atcggggatc gcggcttcac
     1621 cactctgggc gcggtcaccg agccggtgct acaggcgttg ggcattcgct catacatcct
     1681 gcgcgggact gacgagatcg acgaaaccat agccggcgcg gaggttctcg ccgaggattc
     1741 aaaaagaccg gttgccgtgc tcttaactaa gagcgtgctg ggagtgaaat gatgcaacgc
     1801 tacgattatc tgagggctgt agccggcgat accggcgatg cgttgacggt gtgcacgggt
     1861 tggggcgcgc gcgagtggtg ggcggttcga cccagcgatg gcaatcttaa gacacgcaca
     1921 ttggggttag tctcttcgat tgccgccggc cttgctctag cattgcccca tcgaaaggtg
     1981 atcgcaatag acggcgacgg tgcttttctg atgaatctct gcggactgcc cactatcgct
     2041 ctacaaaatc cgccgaatct gatccatttg ctttttgaca acggagttta tgaagcctcg
     2101 ggcggtaccg caacggcttc tcatattgct gatgccgtgt cactggctaa aggggcggga
     2161 tacccgcatg cctgctgggt caagaatccg gaggagttta gagctagatt tctcgaagct
     2221 ctcgatggca atcgtctgat cttgatcgcg gttaaggtag atcccggaca gccgaaagat
     2281 ttgccgccat taaagcttga cgagttagag aacaagtatc tgttcatgcg ctacctggag
     2341 cgaaccgaga aaaaagcgat attgagcccc ggcttcgact ccaaaatata atctcccgct
     2401 caccaaaagc aggatctaca atgctcgatg agtgacactt acctcggttt tcgacgcacg
     2461 tgaattggac atttcggagc ccacggctct gtgcttgcgc ggaaagccaa gccaactcca
     2521 ctgatcggga tagcgtcgga taacatcctc gagatggcgc gtgtaaagcg ccgtcgttgc
     2581 cgtgatggtt tcttcgaggc tctctcgatg cacggcttcg atagcccggc cgatcgccag
     2641 aacgatagag ccgtcgggct gtcgggtggc aaacatgggc aaggtcaccg caccggtgcg
     2701 caatgccaga gtcgccgggc cacgcggcgc cggtaaattt agacccatga attcaaccat
     2761 gatctcgccg gatttgaact catccgcaat gaccaagacg atccgattct cgcgcagtgc
     2821 ttggacaatc gcgcgcaccg cttcacgccg tggcttagcc gaaatcgtgt cgatgcctac
     2881 ttgcgcccga tatctcgtga ttagctgtgc gaattcctcg ttgctggggt gtttcaccac
     2941 aacactgaac ggataacccg ccgcggccaa ccgcgcgccg atgagagcga aattgcccag
     3001 gtgagcgctc aaagcaagca caccttttct ttgcgccaga gcgtcgcgca agttttcttc
     3061 accttcaact cggatttttg caatgattgc ttctttcggg taatcgatta aggccccggc
     3121 ctcaaagagc tcgcgcgtaa agctcttcca aacgcgccac accattgcct tacgtgcggc
     3181 tttgttcggg aaacgatcac cggccgcggt cgcaatattc tgctccatcc gaatccggta
     3241 tctccacagc agcttatggc ttatccaagc ccaaaaagtt gcggcggaaa ccaccctacg
     3301 atccggcaac cgaccgagta cgaaaataaa ggcacgcaaa acgtaagcgc caagtccata
     3361 tctcagacga tagaaaaatt ggcttacagc cgaacggttt tttgatctgc ggcgcttcat
     3421 ggatagattt atgaatgcgt tttcgcagct ttgcaatcca ggaaggaacc gaacaaaaaa
     3481 aggcggcgaa cgccccaggc attcaccgcc ttaatccctc ctaggcgatt aaagccggct
     3541 aacggctcgc agctttacgc atccgctgag tcgacctacg cggaactgaa acttcacttt
     3601 tccggcgagt tgttcttgcc gcaatttggt agattttggc cttggaatga ctctcaagct
     3661 tctcggcttt ccagcgcatc agcccgtcgc ggagataact aggctcgaag ccgagcatct
     3721 cgcaaacgtt tgcaaacgag aaaagccaat cggtgtcttc gtccaagatc cattcttcgg
     3781 cttcgagaaa tagaactcta cccttgccat ccctggctaa caagtacttc tg
//
LOCUS       DASUTG010000636         8071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2174536, whole genome shotgun sequence.
ACCESSION   DASUTG010000636 DASUTG010000000
VERSION     DASUTG010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174536"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="VF089_14630"
     CDS             <1..1904
                     /locus_tag="VF089_14630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6175243.1"
                     /translation="RPLIVAYGISIGSFLVLTRVRNLQSFGHAQIGFDVILITGIIWI
                     TGDAASPFSFLYNLAVMNAAILLFYRGAFFGAGFSSLCYAGLLIWVSYFTQNSGLPLS
                     SSSLIPVVLNIGSFFVIAGLSGFLANKLGETERLLEEKKIDYQELDALKEALMQGMAS
                     GVAITDGKGRINYFNLQAQNLTSLNESAVLGKRLDEVFTSLRWNFDKPQPGTSIILEE
                     IIFAGPEGAQKNIRLSLASLNNSNEECMGYVSIFEDITRQKQFEEKVRVEEELRRARE
                     FNLQQTPLVSSQEDFHFNGIIGKGGGIEAIHQLIRKVALTRTNVLITGESGTGKELVA
                     RAIHLNGPRKTRPFIAVNCGAIPEGIIESELFGHVRGAFTGAVADHPGLFKQADQGTI
                     FLDEVGELPVHLQVKLLRVLQETTFTPVGGNKAIKVDVRFIAATNRNLRREMEQGRFR
                     EDLFYRLNVVQMTMPALRDRREDIATLAHYFMGKFASIHDKKIEEISSSALMHLLAHH
                     YPGNVRELENIIEHAVTVTNKNILTDDDLPGYINASPVAEDCQVVETTAPDIANVFFD
                     KGLSLDAELEAHEKCILVGALQRANGVQKRAAEILGINYRSLRHRLEKYGLLGSKGSS
                     LPNELTEQQ"
     gene            2229..2612
                     /locus_tag="VF089_14635"
     CDS             2229..2612
                     /locus_tag="VF089_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6175244.1"
                     /translation="MKQLRLQNDSGFTLIELLVVVAIIGILAAIAIPQFSAYRKRGYE
                     AAVKADLRNAATGQEAYFTTAFTYRAGVLTSGNLTGFNASPTVAVASGGGSNTYELTA
                     THQNCSTTVWTFSSGNGQISGGPCP"
     gene            2912..5077
                     /locus_tag="VF089_14640"
     CDS             2912..5077
                     /locus_tag="VF089_14640"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX6175245.1"
                     /translation="MGMGFPLLADFQSAPFYPPHLVFLFVSFFDAIRILFVFHYVVSA
                     LGAYALARQWGYPAYLSTIAALLFTFGGIVVSLTNVLNHFQTAVWLPWLILAGARAIR
                     FYSGKNLIMLSVVGLIQFLAGSPELYAISLVLLYVSMVAEAKESNALSYLKPFALILL
                     ANTVIAGVSMVQLLPTLELFVQSRRPTNIPYHEATAWSLSPVSLVNLFILDKEVDRSL
                     FNGVRLFFGRDIPFLVSLYMGQLCVFGLCSWFSHARRFEKLVLSSLVLLGLALALGKH
                     SLIYSLLLAYVPLFSLFRFPEKFVFLVYPLLIIITLRGFHAYVTSEDGTHKKSLRLFC
                     AIVVSLTIIYCVCLLRLDYFFTLMHWADLVRPASIEDILPSLLFSLERQILLSLALLL
                     VLFLYKRRTLGPPVFRMLIVALVFIDLTSAHRQFQFLVRPGLVTDNPKIISNSDSERY
                     RLFSGLPSLHPSVFTMRDRAFPEVAEAVWSSLVPNIGVFYNVDYMQEIDALSRAPYDV
                     FLKTAQNLAPGKLYSLLGALNVKYISSYSPLPEGETELVRHFPEYPLWLYELKRAVPR
                     VYVVSAAEYESDPSKTLERLASEDFDALETVLVHESISFGNKRGVQGEARILAYENHL
                     VNIQASLDIPGILVLADSFYPGWNAYVNGRQEKIYRANLFFRAVPLTAGTHTVEFRYE
                     PRSFAIGRAVSIITLLSILATSIFLYLRKRRRNLTAPGC"
     gene            complement(5058..6644)
                     /locus_tag="VF089_14645"
     CDS             complement(5058..6644)
                     /locus_tag="VF089_14645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6175246.1"
                     /translation="MIPTGLFLGVLLLTVATLKDYGIAWDEPPYFHAADLHIGWILDW
                     GANIDKGNFNQSLSDENIKAAWRWDPYHVPHPPFSRIVSGVTKHVSSGWLDKSSGYRM
                     APALFFAILVAVMFLWMKALFGLATGLFSALALVAIPNIFGFAHIAVTDLPLASMWFL
                     AAFCFWKGLQDWKWSVVCGVVWGLGLATKFPAILIPIPLILWAHLFHRSRYANNVFSM
                     LFLGPSIMIAIQPYLWHQSGLRVLEFLYEGLSRGYRADANFPVYFFDQLYFTHQLPWY
                     YPFFLVGVTTPEPLVFLAALSLVSLIWFRKGTSTVALFALNAFFILGLGLLPGAVLHD
                     GVRQMLPALPFLAATAGSGFYIIGEWLLSLVHRSTQLQAIGNLRGKLIAVLGLLILFS
                     PALDLYLSHPFQLSFYNRFVGGVRGAYQRGLELTYSMEAFTPEFLNAINTKLPQNSVV
                     NASFANFMFNYYQSEGRLRADIKISDAESPDYYILLNRRSVLAPRERLLMNGSTPIYL
                     AEQVAGVPLVAVFELNTPGR"
     gene            complement(6722..7444)
                     /locus_tag="VF089_14650"
     CDS             complement(6722..7444)
                     /locus_tag="VF089_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6175247.1"
                     /translation="MIRTSETSYIGGELHLFSLATNWKRYVKQAIREYLIGDVLEIGA
                     GIGSTTAAMHDGIAHRWVCLEPDLSNATRLKRLVVERSEYKAEVIAGSLDALAKRSSF
                     DCILYMDVLEHIQDDRAQIQAAAPLVRQGGHIIVLSPAHQWLFSEFDKSIGHLRRYNK
                     QVLRSLMPPGWIEVKLAYLDSVGALLSLGNAVGLRQAMPSPWQIAIWDRLCIPLSRIV
                     DSALADNCGKSILAIWRKPDSL"
     gene            complement(7428..>8071)
                     /locus_tag="VF089_14655"
     CDS             complement(7428..>8071)
                     /locus_tag="VF089_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX6175248.1"
                     /translation="RLIAECRAHQDQKIIFAARTRRSASLSFKTFYHLYRLIHFLLTG
                     IQVQVGNFSVIPASALRRLVAVSELWNHYAAAVYKAKLPMALIPAQRNCRLEGSSRMS
                     LVALVVHGLSAMAVFGDRIGVRLLILVSVGMSLVSVALITVIGIRLLTTLAIPGWATY
                     VTGILLVILIQMLIIVLVFAFVILTSRNTVSIIPSRDYVHIADAVRRVYDSNQ"
BASE COUNT         2065 a         1981 c         2013 g         2012 t
ORIGIN      
        1 taagacctct catagtcgct tatgggattt ctatcggctc gtttctggtc ctgacacgag
       61 ttcgtaacct tcaatcgttc ggtcatgctc agatcggctt tgatgtaatt ctgattaccg
      121 gcatcatctg gattacaggc gatgccgcca gcccgttctc tttcctctac aacctagcgg
      181 tgatgaacgc agcgatattg ctcttttacc gcggcgcgtt cttcggtgct gggttttcca
      241 gtttatgcta tgcagggctt ttgatctggg tcagttactt cacccagaat agcggccttc
      301 ctttgtcatc atcttcgctg atacctgtgg ttctgaacat cggcagtttc tttgtgattg
      361 ctggtttgag cggtttttta gccaacaagc ttggtgagac cgaaagatta ctggaagaga
      421 aaaagatcga ctatcaggaa ctggatgcac tgaaagaagc gttgatgcaa gggatggcaa
      481 gcggcgtggc aatcaccgac gggaaaggaa gaatcaacta cttcaatctc caggcgcaga
      541 atcttacttc tttgaatgaa tcggcggttc tcggcaagcg actcgacgaa gtttttacaa
      601 gtctccggtg gaacttcgac aaaccacaac ctgggacaag tatcatttta gaagaaatca
      661 tctttgctgg tcctgaagga gcccagaaga acatccgcct tagtttagcc tctttgaaca
      721 actctaacga agagtgtatg ggctacgttt ccatattcga agacatcacg agacaaaaac
      781 aattcgaaga gaaagtcagg gttgaagagg aactccggcg agctcgcgag ttcaacttgc
      841 agcaaacgcc gcttgtgagc tctcaagagg actttcactt caatggcatc atcggtaaag
      901 gaggaggtat tgaagctatt caccagctga tcaggaaagt ggccctcacc cgaactaatg
      961 tattgatcac cggcgaaagc ggaaccggca aagagttagt agcgcgggcg attcacctta
     1021 atggacctag aaaaacaagg ccgtttattg ccgtgaactg cggtgccata cctgagggta
     1081 ttatcgaaag tgaactcttt gggcacgtcc ggggcgcatt tacaggggca gttgccgatc
     1141 atccggggct ttttaaacag gcagaccaag gaaccatttt tcttgatgag gtaggcgagt
     1201 taccggtgca tttgcaagtc aaactgctga gggtactgca ggaaacgacc tttacgcctg
     1261 tcggcggcaa caaagcgatc aaagtcgatg tgcgttttat tgcggcgaca aaccgcaatc
     1321 tgcggcgtga aatggaacaa gggcgttttc gtgaggacct tttttaccgg ctaaatgtag
     1381 tgcaaatgac tatgcccgcg ttgcgcgaca gaagggaaga tattgcgacg cttgcccact
     1441 actttatggg caagttcgca tctattcacg acaagaagat agaagaaatt tccagtagcg
     1501 ccctgatgca cctattggct catcattacc cgggaaacgt cagagagctg gagaatataa
     1561 tcgaacatgc cgtcactgtg accaataaaa acatcctgac agatgatgat ctaccagggt
     1621 acattaatgc cagccctgtt gcagaagact gccaggttgt tgaaacaact gcgccagaca
     1681 tcgccaacgt gtttttcgac aaaggcttgt ccttagacgc cgaactggaa gctcacgaga
     1741 agtgcattct cgtaggtgcg ctccaaagag ccaatggcgt tcagaagcga gcggcggaaa
     1801 tcttagggat caactacaga tcactgcgcc acagactcga aaagtacggt ctgttaggca
     1861 gcaaaggaag ttcactaccg aatgagctta cagaacagca gtgacaattt ttgttacttt
     1921 gaattctgct caacttccat aatcccgaaa tccggctgct gctaattgcc tcctcccacg
     1981 acgctcggtc actggtgggt cattagccgg aaaactgtac gcttcagtga cacggcttgc
     2041 ctgagatttc tgatgcagtc ccgcccagct cagatggctc tgcttttgct caatcggcga
     2101 ggtcaacgtc tgaacgtcaa aacagcacga aggaggtatg cgacagaaag cagcggtcaa
     2161 ttaggttaga acatctcgaa ggaaattgtt gcaactaaca tttagtacga aaggaacaca
     2221 aacactcaat gaagcagtta cgcttacaaa atgattccgg ttttacactg atcgagcttt
     2281 tggtcgtggt agcgatcatc ggtatattgg cggccattgc cattccacag ttttcggctt
     2341 atcgaaaacg aggatatgaa gcggcagtga aagccgatct gagaaatgca gcgacaggcc
     2401 aagaggcgta ttttaccact gcgttcacct atagggcagg agttcttacc agtggcaact
     2461 tgacaggatt caatgcgagt ccaacggtag ctgtggcatc aggcggtgga tcaaacacgt
     2521 atgaattgac ggctacgcat caaaactgtt caaccacagt ttggaccttt tccagtggaa
     2581 acggccaaat cagcggcgga ccctgtccgt aaccatccgg cttcaatcta ccttgatcga
     2641 tcactaccag gggaggggat tcacatcctc tcccctttgc ctattaagag catggcgctg
     2701 cggtatcgtc gctattgaac gatgcagaaa tgtattcgcg aatccgagcg ccttgactgt
     2761 cctgctcatt gggatttctt acttcactca cgagatgatc tgggacgccg aagtgccctt
     2821 ttttcgggat ctgggtccgt atttttatcc tatcaggtac agcttggctc agagtctcag
     2881 cgccggagag ctaccactct gggatcggca catgggcatg ggctttccgc tccttgcaga
     2941 ttttcaatcg gcgccgtttt acccgcccca cttagttttc ctttttgttt ctttttttga
     3001 cgcaatcagg attctctttg tatttcatta tgtagtttct gcgcttggag catatgcttt
     3061 agcccggcaa tggggatatc cagcttacct ttcaactata gctgctttac tcttcacgtt
     3121 tggtggaatc gttgtttctc tgacgaatgt gctgaatcac tttcaaacag ccgtatggct
     3181 gccgtggctg attctcgctg gagcacgggc gattcggttt tattccggaa aaaacctcat
     3241 aatgctttcc gttgtcgggc tgatccagtt cttagcgggc tcaccggagc tgtacgccat
     3301 tagcctcgtg ttactctatg tttctatggt agcggaggcg aaagagagca atgctctgtc
     3361 gtacttaaag ccattcgcac tgattctact agctaataca gtcatcgcag gagtgtcgat
     3421 ggtccagctt ttgccgaccc tcgagctatt cgtccaatct cggagaccaa ctaacattcc
     3481 ttatcacgaa gcaacggctt ggtcactgag cccggtgagt ctcgttaatt tgtttattct
     3541 ggacaaggag gtggatagga gcctgttcaa tggcgttcgg ttgttctttg gccgtgacat
     3601 tccgtttctg gtgagtttgt atatggggca actgtgcgtt tttggcctct gttcatggtt
     3661 ttcgcatgct cgacgtttcg aaaagctagt gctctcgtca ttagtgcttc tcggacttgc
     3721 acttgcttta ggaaaacaca gcctcattta ctctcttctc ttagcgtatg tgccattatt
     3781 cagcctattt cgcttcccag agaagttcgt tttcttagtt tatccgctct tgattatcat
     3841 cacgcttcgg ggcttccatg cgtacgttac ttcggaagat ggcacccaca agaaatctct
     3901 tcggttgttt tgcgccatcg ttgtctcgct gacgataata tattgcgttt gtcttctgcg
     3961 gctggactat tttttcacct taatgcattg ggcggatctg gtgaggcctg catctatcga
     4021 ggatattctt ccttctttgc tgttcagcct ggagcggcag attctattat cgctagcgct
     4081 tctgctagtt cttttcttat acaaacgacg aacactcggc cctccggtgt tccggatgtt
     4141 gattgtagcg ctggtattca tcgacttaac gtcggcccat cggcagttcc agtttcttgt
     4201 tagaccggga cttgtgacgg ataaccccaa aattatttca aattccgact ccgaaaggta
     4261 ccggttgttt tctggtcttc cgagtcttca tccaagcgtt tttacaatgc gcgatagagc
     4321 cttccctgaa gtggctgaag ccgtgtggtc ttcactagtt ccgaacattg gtgtttttta
     4381 taatgttgac tacatgcagg agatagatgc gcttagccgg gcaccttacg atgtatttct
     4441 caaaacggcc caaaatcttg cccctggaaa gctctattcg ctactcggtg ctcttaatgt
     4501 gaagtacata agcagctact ctccgcttcc agagggggaa actgagctgg tgcggcattt
     4561 ccccgaatat ccactgtggc tgtatgaatt gaagcgggcg gtacctcgag tctacgtcgt
     4621 ttcggccgcc gaatacgaaa gcgatcccag taagacgtta gagcgacttg caagcgagga
     4681 ttttgacgcc ctagaaactg tcctagtcca cgagtcaata tcattcggca ataagcgtgg
     4741 agttcaaggt gaagctcgaa tactcgcgta tgaaaaccat ctggtaaata tccaggcttc
     4801 gctggatatt cccgggatcc tggtgttagc ggattcgttc taccccggtt ggaatgctta
     4861 cgtgaatggg cgacaagaaa agatttatag ggcaaatctt ttctttaggg cggtgccgct
     4921 aaccgctgga actcacactg tcgagttccg ttatgagccc cgttccttcg ctataggtcg
     4981 tgcggtctcg attatcactc ttttatctat tctcgcaact tccatctttc tctatttgcg
     5041 aaagcgcaga cgaaatctta ccgccccggg gtgttaagct cgaatactgc caccaacgga
     5101 acacctgcaa cctgttcagc caagtaaata ggtgtgctgc cgttcattag cagacgctca
     5161 cggggtgcga gcacgctccg ccgattaaga agaatataat agtcaggcga ttcagcatca
     5221 gaaatcttga tgtcggcacg aaggcgtcct tcgctctgat aataattaaa catgaagttg
     5281 gcaaaagaag catttacgac agaattctgc ggcagctttg tattgatcgc gtttaggaat
     5341 tccggggtaa atgcttccat agagtatgtg agctccaaac cgcgttgata cgccccgcgg
     5401 acgccgccga caaaacggtt gtagaagctc aactggaaag gatgagacaa gtaaagatcc
     5461 agcgcgggcg agaacaggat gagcaacccg agcacggcga taagctttcc acgcaggttt
     5521 ccaatagctt gcaactgtgt cgatctatgc accaaggata agagccattc tccaataata
     5581 taaaacccgc tcccggccgt tgcggccagg aatggtagag cggggagcat ctggcgtacg
     5641 ccatcgtgca gtaccgcgcc cggcaaaaga ccaaggccta gaataaagaa cgcgttcagg
     5701 gcaaagagcg ccacggttga cgtgcctttt cgaaaccaaa tcagcgaaac tagactgagt
     5761 gctgctagaa agacgagagg ctccggtgtc gttacgccaa ccaaaaaaaa aggataatac
     5821 cagggaagtt gatgcgtgaa gtataattga tcgaagaagt agaccgggaa gtttgcatcg
     5881 gcccgatatc cgcggctcaa tccttcgtag agaaactcca aaactcgaag tccggattgg
     5941 tgccacaaat aaggttgaat cgcgatcata atggacggtc cgagaaagag cattgagaaa
     6001 acattattgg cgtagcgact ccggtgaaac agatgagccc acaagatcaa tggtatcgga
     6061 attaagattg cgggaaattt ggtcgcaagt ccaagccccc ataccacgcc gcaaaccaca
     6121 ctccacttcc aatcttgaag gcctttccaa aaacaaaagg cggctagaaa ccacatggag
     6181 gcgagtggca agtcggtaac ggcgatgtgt gcaaaaccaa agatattggg tatggccact
     6241 aaggccaatg cagaaaaaag acccgttgcc aaaccaaaca gtgccttcat ccacagaaac
     6301 atcaccgcaa ccaagatcgc gaagaatagc gccggagcca tacggtaacc cgaagactta
     6361 tccaaccatc cggatgatac gtgttttgtg acaccggaaa cgatgcgcga aaagggcggg
     6421 tgcggcacat ggtacgggtc ccagcgccaa gcagctttga tgttttcatc gctcaggctt
     6481 tggttaaaat tccctttatc gatgtttgct ccccaatcga gaatccaccc gatgtgtaga
     6541 tcggcggcat gaaaataggg cggttcgtcc caagctattc cataatcctt cagggttgcg
     6601 acagttaaaa gcaaaacgcc cagaaacagg ccggtgggaa tcatccagcg cactaacgtc
     6661 tcctcgcccc tcaatgagtt gggtgcatgc tgagataggt catgattgta gaagtcgtaa
     6721 gttacagaga atccggttta cgccatatcg ccagtatgga tttgccgcag ttgtctgcta
     6781 gggcggaatc cactattcga gaaagcggta tacaaaggcg atcccagatc gcaatctgcc
     6841 aaggggacgg cattgcttgg cgaagtccta cggcgttccc caacgacaac agagctccaa
     6901 cagaatcaag atacgccaat ttgacttcta tccaccccgg aggcatgagg gaacgaagga
     6961 cctgtttgtt ataacggcgc agatgcccaa tgctcttgtc gaattcactg aacagccact
     7021 gatgggcggg cgataagacg atgatatggc caccctgccg aacaagcggt gcagcggctt
     7081 ggatttgagc gcgatcatct tggatgtgct cgagaacatc catatacaag atgcaatcaa
     7141 aggatgagcg tttcgctagc gcatccaatg accccgcaat gacttcagcc ttatactccg
     7201 agcgttcaac aaccaacctc ttcaatcggg tagcgttgct taggtctggt tcgaggcaca
     7261 cccacctatg tgcgatgcca tcgtgcatcg ctgcagtggt gctgccaatc cctgcgccaa
     7321 tttcaagtac gtccccgatc aagtactctc tgatcgcctg ctttacatac cgcttccaat
     7381 ttgtcgcgag agaaaagaga tggagctcgc cgccgatata agaagtttca ctggttcgaa
     7441 tcatagaccc gccgaacggc atccgcgata tgaacatagt cacggctggg aatgatgctg
     7501 acggtgttac gactagtgag aatcacaaac gcaaagacaa gcactatgat gagcatctga
     7561 atcaatatta ccaacaagat ccccgtaacg taggtagccc agccgggtat ggcgagggtc
     7621 gttaacaatc ggataccgat aacggtgatt aaagctacgc tgactaggct cattccgaca
     7681 gagacaagta tcaacagcct gactccgata cggtcgccaa agactgccat agcactcaat
     7741 ccgtgaacca cgagagcaac taagctcatg cgtgacgatc cttcgagcct gcaattacgc
     7801 tgagctggaa tgagagccat cggtaacttc gccttataaa ccgctgcggc atagtggttc
     7861 cagagctccg aaacagcaac gagtcgtctg agagcgctgg cgggtatcac actgaagttg
     7921 ccaacttgta cctgaatgcc ggtcaacaag aaatgaatca accggtacag gtgatagaag
     7981 gtcttgaatg ataaacttgc cgagcgcctg gttcgtgcag cgaagataat cttttgatcc
     8041 tgatgcgcac gacattctgc aataaggcgg g
//
LOCUS       DASUTG010000637         5356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_691839, whole genome shotgun sequence.
ACCESSION   DASUTG010000637 DASUTG010000000
VERSION     DASUTG010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691839"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..960)
                     /locus_tag="VF089_14660"
     CDS             complement(169..960)
                     /locus_tag="VF089_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6175249.1"
                     /translation="MIELFHNDMSVCAQKVRFALGEKMLDWKSHHLNLRAGEQQKPEY
                     LKLNPNAVVPTLVDSGRVIIESTVINEYIDDAYPEPRLRPAGAGERARMRLWTKQLDE
                     GVHVATRIISNAIAFRHQKLALGPEALKMLHDKIPDPKKREESWENITKGIESRFFVD
                     AVRRFNRLLADMEGSLAEESWLAGKEFSLADIGYAPYIRRLQDLQLQFLWDKRPHISG
                     WSERVAKRPGYRQAFDDWPNEAYASLMKEKGGEAQSRIRAILAAA"
     gene            complement(1119..2000)
                     /locus_tag="VF089_14665"
     CDS             complement(1119..2000)
                     /locus_tag="VF089_14665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175250.1"
                     /translation="MSKLFPPLLFCLTLFSGTVAQAQERFSFFQASTVESVTRMLRFA
                     GIRDDDVLVDLGSGDGLIPITAAQMNPKLRAWGVDVDEKLIRESNQAAKAKGVADRVT
                     FFHRNAFDADLREATVVTMWLFPELMRLLRPTILKTARPGTRILTSTWDLGSWAYDEM
                     DTGNPTIYKWIVPARVEGYWSWQLPIAGQQITYSSVMEQRFQTVEAVVRAGKYRELLQ
                     DIKLRGEDISFALAMTLDGVGHTRHEFRGKVRGDQIEGRAIVTLENHRKLELPWHAMR
                     TRTSLYFAPTGTDVKTD"
     gene            complement(2252..2398)
                     /locus_tag="VF089_14670"
     CDS             complement(2252..2398)
                     /locus_tag="VF089_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175251.1"
                     /translation="MALLLQKKGITRVRPLDGGIDAWLSNNFPCETLVVVTESSTGVP
                     ALSQ"
     gene            complement(2423..3229)
                     /locus_tag="VF089_14675"
     CDS             complement(2423..3229)
                     /locus_tag="VF089_14675"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6175252.1"
                     /translation="MNESIQFLIEHGYILLFAWVLIEQMGLPIPAVPLLIAAGALAGS
                     GKINLALAMGSAIIAVLLADVFWYYLGSYRGGRVLKLLCRISLEPDSCVRRTEDVFAR
                     HGAHSLLIAKFVPGLNTAAPTLAGIFRMPLPRFMVFDGLGAFFWVSTFAALGYGFSDQ
                     LEWVAAYFLRWGSWLLMALLGGLAAYILWKYFQRQRLLYRLRIARISPKELLEKINAG
                     EQVMIVDLRQPLDIEIVPYVIPGALRMAMEELEDRHHEIPRDREVILYCS"
     gene            complement(3769..4465)
                     /locus_tag="VF089_14680"
                     /pseudo
     CDS             complement(3769..4465)
                     /locus_tag="VF089_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
     gene            complement(4640..4915)
                     /locus_tag="VF089_14685"
     CDS             complement(4640..4915)
                     /locus_tag="VF089_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX6175253.1"
                     /translation="MAQPCEHLSGLASGDFPPQRTPDACEECLKEGTLWVALRECRSC
                     GHVGCCDSSTGKHATKHFHDTQHPVMRAVPPASWVWCYVHKAQGVLA"
     gene            complement(5022..>5356)
                     /locus_tag="VF089_14690"
     CDS             complement(5022..>5356)
                     /locus_tag="VF089_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX6175254.1"
                     /translation="AVVMVTEPRMPCYKLGIKFGRSDILKKFLASERTGFYFAVLQEG
                     EVGAGDPIELIEKSDHGVRVSDITRLYTREKRNLGLLRRAVEVEALPESWKSYFHQRI
                     AKLVDHGG"
BASE COUNT         1213 a         1583 c         1357 g         1203 t
ORIGIN      
        1 gttcatcggg agctaaatct cttagtgctg ctcgcaccat ctcgcgcacc cggcccagag
       61 cgtctagtac tagcgccgcg gcttccatcg ctacctccaa gtctcggttc caatcgttcc
      121 agtcgttcca gctgttccaa ccgcttaaac cgtgcaaacg ggtacggtct aagccgctgc
      181 aaggattgct cggatccgcg actgtgcttc accgcctttt tctttcatga gcgaagcgta
      241 agcctcattg ggccagtcat cgaaggcctg ccggtatccc ggtcgtttgg caactcgttc
      301 cgaccatcca gaaatatgcg gccgtttgtc ccacagaaac tgcagttgga gatcttgcaa
      361 ccgccggatg tacggagcgt agccgatatc ggccaatgag aattcttttc cggctagcca
      421 ggactcttct gccaaactac cttccatgtc ggccaggagt ctgttgaagc gacgcacggc
      481 atcgacgaag aaacgcgact cgatgccctt ggtgatgttc tcccagctct cctcgcgttt
      541 tttgggatcg ggaattttat catgaagcat tttcaacgct tccggtccca gggcaagttt
      601 ctggtgacga aacgcaatgg cattgctaat gattctggtc gccacatgaa cgccttcgtc
      661 aagctgcttg gtccagagcc gcatgcgcgc ccgttcgccg gcaccggccg gacgcaggcg
      721 cggttcggga taagcatcgt cgatgtattc gttgatgacc gtcgattcga tgatgactct
      781 gccgctatcg accaaggtcg gaaccacggc attcggattg agctttaagt attctggttt
      841 ctgctgttcc ccggcccgca ggttcaaatg atggctcttc caatcgagca ttttttcgcc
      901 gagagcgaag cgcacctttt gcgcacagac cgacatgtca ttatgaaaaa gctcgatcat
      961 cgcatggatc ctcccgccga tcgaaagact cgatgcagtt ccaaccgttc cagccgttca
     1021 agtcgttcaa ccgggctaac cgtccagtat cgcaagatcc aacgttcaga agctagaccc
     1081 aatttaacac caccatcaaa catctcagac gcagagcatc agtcggtctt cacgtcggtg
     1141 ccggtggggg caaaatagag tgacgtcctc gtccgcatag catgccacgg cagctccagt
     1201 tttcgatggt tctccagagt cacaatagct cttccctcga tctggtcgcc gcggaccttg
     1261 cctctgaatt catgccgggt gtggcccact ccatccaggg tcatcgctag cgcaaaggag
     1321 atgtcctcac cgcgtagttt gatatcttgc agcagctccc ggtatttgcc cgcgcggacc
     1381 acggcttcga ccgtctggaa ccgttgttcc ataaccgacg agtaagtgat ctgctgtccg
     1441 gctataggca gctgccagct ccaataccct tcgactcgcg ccggcacgat ccacttgtaa
     1501 atcgtcggat ttccggtgtc catctcgtca tatgcccagc tgccaagatc ccaggtgctg
     1561 gtaagaatgc gtgtgccagg acgcgccgtt ttaaggatcg tgggacgaag caatcgcatc
     1621 agttccggga atagccacat ggtaacaacc gttgcctcac gtaaatccgc atcgaaggcg
     1681 ttccgatgaa aaaacgtcac ccggtcggcg acacccttcg ctttggcggc ttggttggat
     1741 tcacggataa gcttctcgtc gacatccact ccccaggcgc gcagttttgg attcatctgc
     1801 gccgcagtga tggggataag cccatctccc gaaccgagat cgaccagcac atcatcgtca
     1861 cggatgccgg caaatctcag catgcgggtt acactctcga cggtactagc ttggaaaaag
     1921 gaaaagcgct cttgtgcttg cgctaccgtc ccagagaaca acgtcaggca aaacagaagc
     1981 ggcgggaata gtttcgacat gttgcatctc ctcgctgatc aaccaggaac tccacagtga
     2041 tgtaacatcg atcagcgatt ccgccgcgcc gggatcgaca tcgttccatc caatgccttc
     2101 cccactcgat cgttgccgct gaccagacaa gtttcatctc tactcgggaa tccgttcatc
     2161 cggaaaataa ttagcgattc ccgcgcgctt ggtcaatctc agagatcgcc cggtgcacgc
     2221 agacatgatc ggttaatagg gcaatccgaa tttattgcga tagcgcaggc acgccggtgg
     2281 aagattcggt gacaacgacc aaagtttcgc atgggaagtt gttgctgagc caggcatcga
     2341 taccgccatc gagcggacga actctggtga tgcccttttt ctgcaggagt agcgccaccc
     2401 gggcactggt cgcttcatta ggtcacgaac agtagagtat aacctctcgg tcgcgcggaa
     2461 tttcgtgatg acggtcctca agctcttcca ttgccatgcg aagagcgccg ggtatcacgt
     2521 acggaacaat ttcgatgtcg agcggctggc gcaggtcaac aatcatcacc tgctctccgg
     2581 catttatttt ctccagtagt tctttcggac tgatacgcgc gatgcgcagg cgatagagca
     2641 agcgctgtcg ttggaaatac ttccaaagaa tataagccgc caagcctccc agtaaggcca
     2701 tcagcaacca actgccccag cggaggaagt aagccgcgac ccattcgagt tgatcgctaa
     2761 agccgtagcc cagagctgcg aatgtcgaga cccagaaaaa tgcccccagg ccatcgaata
     2821 ccataaagcg cggtagcggc atgcggaaaa tacccgccag tgttggggca gcggtattca
     2881 gaccagggac aaactttgcg atcagcaatg aatgagcgcc atgtcgtgca aagacatctt
     2941 cagtccggcg cacgcatgaa tccggttcga gagatatgcg gcatagcagc ttcaatacgc
     3001 gaccgccgcg gtagctgccc aggtaatacc aaaaaacatc agccaatagg accgcgataa
     3061 tggccgagcc catagccagc gcgagattga ttttacccga ccctgcaagc gcgccggcag
     3121 caattagcaa cggcaccgcg ggtatcggca agcccatctg ctcgatcaac acccacgcaa
     3181 acagcagaat ataaccgtgc tcaattagaa attggatcga ctcgttcaac aatgatctcc
     3241 gagattacca gattgcaaat gtttcaatcc tttgcgaact cagcatccga gctagtctgg
     3301 atcggatcaa ccgagactcg cttcagttat tttctctaag tttgaaccag accagggcgt
     3361 taaccagtac ttacccggcc gctacgacgc gcaccacggt gcctgatcgc acatgaatat
     3421 actcctccag gtgtgctccg cattttctcc tactttccaa tagcagaatc tttaatcctg
     3481 cggaacgacc ttgcggagca attcaagggg gtagtcgaga agctttcgag atagcgaggc
     3541 agaaattgtc tctcaagatt gtctaccgtg ctccacccga tccggaacgt ccgaaacctt
     3601 ttcggcgttg ataaagttac gagaacctag aaccgcgaaa ccacccatag gcaaccggcg
     3661 accgcgacta gatcttcagc aacagcggtg tacgtgtccg gtgtattgac ttgttgatca
     3721 tcaccttcaa cttaccacac ggcttgctct gccaaacgat caccgcactc atcggagatc
     3781 gaacaataga atttcacttt gatcattggc tgtaagatcc acggcctgtt cgctggtaag
     3841 agacgcgcca tcgccctctc cgagcaatga gccgttaagc gtcaccttgc cacgtcccac
     3901 ctgcacccat gcctcacggc cgacgcgcaa gctatagttt aacttctctc ctttcggcaa
     3961 aaccgacagc aacagctccg cgtcttgatg aatcttcaac gcgccatcgc gcgcgtccgg
     4021 agcggcgacg agaagccaac ttccgatctt cttgttgaga tcaaaagagc gttgttcgta
     4081 tcccggtttc aggccggttt cgttcggtat gatccagatc tgcaggaaat gcaccggctc
     4141 ggcttgggat gcattgtatt cgctgtggct gatgcctgtc cccgcgctca tgcgttggac
     4201 gtcgccgact cggatgaccg aactcgcgcc ggtactgtct ttgcgccagc gcgccttcca
     4261 gcacgtaggt gatgatttcc atatcgcgat gcgagtgcgt tggaaatcca gcgcccggag
     4321 ccacccggtc ttcattgatc accctgagat cgctgaagcc catctgttgt gggtcatagt
     4381 agtcgccgaa tgaaaatgtg tggcggctgt cgagccagtc aaacttggaa gaacctcttt
     4441 tactgcccgg gcgcaccgtt atcatttttc cctccttgtc gattcactga aagagcggcg
     4501 tcagtcccga acggcgcttc ctgccgatgt ccccacgctt ggccacctga tcctcgagat
     4561 tggcggccgg ggtcgtagtt aatggctgag gaagtgcgcc gatttaattc cccgactcaa
     4621 cgccgctgaa cgtcaagagc tacgcgagga caccctgtgc tttatgcaca tagcaccaga
     4681 cccagctcgc aggcggaaca gcgcgcatca ccggatgttg cgtatcatgg aaatgtttgg
     4741 tggcgtgctt gccggtggac gaatcacaac aaccaacgtg gccgcacgac cggcactcgc
     4801 gaagcgcgac ccacaacgtg ccctccttca ggcactcctc gcaggcgtca ggagttctct
     4861 gcggcggaaa atccccggag gctaaccctg acaaatgttc acacggctgg gccataaggt
     4921 cctccaatgt aaagtgtcaa tgcaccttaa atttccttcg cttcgagacg tttgtctcaa
     4981 tgcccttttg cctcttcttg ctctagccga aaagtatcgc gctatccacc gtgatcgacc
     5041 aatttagcaa ttcgctggtg aaaatagctc ttccagcttt caggcagagc ctcgacttct
     5101 actgcgcgac gaagaagtcc gaggttgcgc ttctcgcggg tatagagtcg ggtgatatcg
     5161 ctgacccgaa caccatggtc gcttttctct atcagttcga tcgggtcacc cgcgccgacc
     5221 tcaccttctt gcaataccgc aaaatagaag cccgtgcgct cgcttgccag gaacttcttg
     5281 aggatgtcgg agcgaccgaa cttgatgccg agcttataac acggcatccg cggttcggta
     5341 accatcacga cagcgg
//
LOCUS       DASUTG010000638         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_542060, whole genome shotgun sequence.
ACCESSION   DASUTG010000638 DASUTG010000000
VERSION     DASUTG010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542060"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..749)
                     /locus_tag="VF089_14695"
     CDS             complement(30..749)
                     /locus_tag="VF089_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6175255.1"
                     /translation="MNYQFNWSILWTGQSGAWLLQGVITTLQLTLLAWCLAVMLGILA
                     GAFRTLPFAPLRWLSSSYVEFFRNVPLLVWMFFWYFAVPPLLPQAVQDWLFDHGAEFW
                     AGMFALGVYHGARFSEVIRAGIQSIPRNQYESAVSTGLTIPQTYRLIIIPIALRLIIP
                     PATNESLNLLKNSSVALTIGVAELTFQTRQIETYTAKALEALTAGTVIYLALCLGLAV
                     FASRLERRFAIPGMIVQVKAD"
     gene            complement(816..1682)
                     /locus_tag="VF089_14700"
     CDS             complement(816..1682)
                     /locus_tag="VF089_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6175256.1"
                     /translation="MKRALPRMILLILLIGLIGGSRSAKAETTLEKIARTGTITIGTR
                     TGSPPFGYINKNNDWVGFSIELVESAIAPVLSKRIGKQLKIEKKESTPQTRIPLLTSN
                     AVDLIAETMTDTQSRRESVDFSITYFVTGAQFLVKKGNAIKGLKDTAGKRIAAQQGST
                     NARIIRERVPTAKLVEFPDQPAAFQALAQGQVQAYTNDGIQLAGLKAKAPRPDEWLVV
                     GEFYSYEPYGMAMRKNESDFRHAVNVGLMDAIESGRYFEIYEKWFGKKGEVPYPMSAE
                     TKRFLQMQVVPK"
     gene            complement(1692..2420)
                     /locus_tag="VF089_14705"
     CDS             complement(1692..2420)
                     /locus_tag="VF089_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6175257.1"
                     /translation="MIEFHQVNKWFGSLHVLRDIDLKIESGEVVVVCGPSGSGKSTLI
                     RCINRLEAIQSGELTVDGLSLTDPNLDTAKLRANIGMVFQSFNLYPHMTALQNITLAP
                     LKVKGLSRLDAEKLATDLLDRVGIPEKASAYPANLSGGQQQRVAIARALAMHPKIMLF
                     DEPTSALDPEMIKEVLDVISALAREGMTMVVVTHEMGFARRVANRIVFMDGGKIVEVG
                     QPEKFYTAPQSARAQQFLSKILSH"
     gene            2607..3065
                     /gene="sixA"
                     /locus_tag="VF089_14710"
     CDS             2607..3065
                     /gene="sixA"
                     /locus_tag="VF089_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX6175258.1"
                     /translation="MEFYLVRHGAAVSQAVNGQRPLTPEGRRDVERLGRAAAQRGIQP
                     RQIFHSGLLRAQQTAEILSESLGPVEGVRELVGLRPDDDPVIVKAELETSTISLMLVG
                     HLPHISRLASFLVAGDADRSVVEFAPATLLGLAYEDYRWEILWKITPATP"
     gene            3144..4430
                     /locus_tag="VF089_14715"
     CDS             3144..4430
                     /locus_tag="VF089_14715"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX6175259.1"
                     /translation="MASDTLTIIDNRTGKKYDIPVQNGAIKATDLFQIKVSEDDGLVS
                     YDPGFMNTASCQSKITYIDGDKGILRYRGYPIEQLAEESTYLETAYLILYGELPSKSE
                     LAEWIGNITIHSIIHENIKKHIDGFHHDAHPMGILISTVAALSTFYPDAKKIFDAKSR
                     REQTYRLISKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPNPVL
                     EKALDVLFILHADHEQNCSTNAMRGVSSSQVDPYSSVAAAAAALYGPLHGGANEAVLR
                     MLMGIGSKQKVPEFIKKVKGGEGRLMGFGHRVYKNYDPRAKIIKNIADQVFEVKGRNP
                     LLDIALELERIALEDDYFIKRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTSGWI
                     AQWEEMLTDPDQKLARPKQVYLGADKRDYVAMDRRS"
     gene            4783..>5067
                     /locus_tag="VF089_14720"
     CDS             4783..>5067
                     /locus_tag="VF089_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6175260.1"
                     /translation="MLVAKAKSSKRPIHFSMLRSYQTADLITLANGAAGMAAILSMMS
                     YLQSPENWRVYLALSLIPVAFIFDILDGKLARRQHKNSVFGQELDSLADIV"
BASE COUNT         1207 a         1320 c         1343 g         1197 t
ORIGIN      
        1 aatgataact tgaaaatcca tggaaaacgt taatcggctt tcacctgaac gatcatgccc
       61 ggaatggcaa agcgccgttc aaggcgggat gcgaaaactg cgagaccgag gcacagtgcc
      121 agatagatga ctgtgcctgc ggtgagcgct tccagagcct tggcagtgta ggtttcgatc
      181 tgtcgtgtct ggaaagtgag ttccgcaacg cctattgtca atgccaccga tgaatttttg
      241 agcaggttca acgactcgtt agtggcaggc ggaatgatca accgcaatgc gatgggaatg
      301 ataatcaggc ggtaggtttg cggaatggtc agcccggtgg aaacggcaga ctcgtactgg
      361 tttcgcggga ttgattgaat accggcccgg atgacttcgg aaaaacgcgc gccgtgatag
      421 acgccgaggg cgaacatgcc ggcccagaat tccgcgccat ggtcgaacag ccagtcttgc
      481 acagcttgcg gcaatagcgg tgggacggcg aaataccaga aaaacatcca gaccagcaac
      541 gggacattgc gaaaaaattc aacgtaggaa gaggatagcc agcgtagcgg cgcgaatggt
      601 agggtgcgaa acgcgccggc aaggatgccg agcatcaccg caagacacca tgccagcagg
      661 gttagctgaa gcgtagtgat tacgccctga agcagccagg cgccggattg accggtccat
      721 aagatgctcc agttaaactg gtaattcatg cgcgagatgc aggacgatga ggcgatttag
      781 gacgttctgc tgcccctgcg aacccgacac ttcgatcact tgggcaccac ctgcatctgc
      841 aggaaccgtt tggtttctgc gctcatcgga taggggactt cgcctttttt cccaaaccac
      901 ttttcgtaaa tctcgaaata gcggccagat tcgatcgcat ccatgagtcc gacattgacc
      961 gcatgacgaa aatccgactc gtttttcctc atggccattc catacggctc atatgaataa
     1021 aattcgccga ccaccagcca ttcatccggt ctgggtgcct tggctttcaa tccggcgagt
     1081 tgaataccat cattggtgta agcttgcact tggccttgcg cgagcgcttg aaatgccgcc
     1141 ggctgatctg gaaactccac cagcttggct gtgggaacgc gttcccgaat gatgcgggcg
     1201 ttggtcgagc cttgctgggc ggcgatgcgc ttgcccgcgg tatccttcag tcccttgatg
     1261 gcattgcctt ttttgaccag aaactgcgcc ccggtcacaa aataagtgat gctaaaatcc
     1321 acgctctccc gccgcgactg ggtatccgtc attgtctccg cgatcaagtc gacggcgttt
     1381 gaggtgagca gcggaatgcg cgtttgcggt gttgattctt tcttttcaat tttaagttgc
     1441 ttgccgattc ttttcgacag caccggcgca atcgcgcttt cgacaagctc gattgagaac
     1501 ccgacccaat cattgttttt gttgatatag ccgaatggcg gcgatccggt gcgagtacca
     1561 atcgtgatgg ttcctgtacg cgcaattttt tctaatgtcg tttccgcttt ggccgatctg
     1621 ctaccaccga tcagcccaat cagcagaatg agtaatatca tgcgcggtaa agctcttttc
     1681 atatctactc cttaatgcga gagaatttta ctcaggaatt gttgagcgcg ggccgattgc
     1741 ggcgccgtat agaatttctc cggttgcccg acctcaacta tttttccccc atccatgaat
     1801 acgatgcgat tagcgactcg acgcgcgaag cccatttcat gggttacaac gaccatcgtc
     1861 atcccttccc gcgccaaagc acttatcacg tccaacactt ctttgatcat ctccgggtcc
     1921 aaggcagacg tcggctcatc gaagagcatg attttgggat gcatggcgag cgcccgagca
     1981 atggcaactc gctgctgttg accgccagaa agattggcgg gataggcgct tgctttctcg
     2041 gggatgccta cccgatcgag cagatccgtg gccagctttt cagcatccaa gcgactcagg
     2101 ccttttactt tgagcggcgc cagggtgatg ttttgtagcg cggtcatgtg cgggtagaga
     2161 ttgaaagatt gaaaaaccat gccgatgttg gcgcgcagct ttgcggtatc gagattggga
     2221 tcggtcaaag aaagtccgtc aaccgtcagt tcgcccgact ggatcgcctc cagtcgattg
     2281 atgcaccgaa tcagagtgct cttgccgctc ccgctgggcc cgcagacgac gacgacttct
     2341 cccgattcga tcttgagatc aatatccctt aatacgtgca gcgatccaaa ccatttgttg
     2401 acttgatgaa attcgatcac ggttacgaag cagattgaaa agccaaatag aatcgttgaa
     2461 cctcggcatc ctatatcaac atgtgctttt tgcaactgga tgtcattcgg ggtcaaaaca
     2521 cgcattctat attactatcg gcattcacag agatctcacc tgcgacatat tgctgtggcc
     2581 ggcagcggtg ttaagttgaa tcgacgatgg aattttatct agtccgtcac ggcgcagcag
     2641 tttcacaagc ggttaacggc cagcgtccat tgacgccgga ggggcggcgc gatgtggagc
     2701 gtttgggccg ggcggcagca caacgcggta ttcaaccgcg ccaaattttc cattcagggc
     2761 ttctgagggc tcagcaaacc gcggaaatcc tgagtgaaag tctcggtcca gtcgaaggag
     2821 tacgcgagtt agtcggactg aggcccgatg acgatccagt gatcgtcaag gcggagctcg
     2881 aaacgtcaac catttccctg atgctggttg gccacttgcc acacatcagt cgcctggctt
     2941 cttttctggt cgccggcgac gccgatcgga gcgtggtgga gttcgctccc gccaccttgc
     3001 tcggtttggc ctacgaggat tatcgctggg aaatcctctg gaaaatcacg cctgcaacgc
     3061 catagagcga gttgaccggc ggaatgggat agtctaaaag gaatcgataa caagaacctg
     3121 ggtgacactc gaaaggtagc gcaatggctt cagacactct gacgatcatc gataatcgaa
     3181 ccggcaagaa atacgatatc ccggttcaaa atggcgcgat caaggccacc gatctgtttc
     3241 agataaaggt ttccgaagat gatgggctcg tcagctacga tccgggattt atgaacaccg
     3301 cgtcttgcca gagcaagatc acctatatcg atggtgacaa gggtattttg cgctatcggg
     3361 gatatcctat cgaacagctg gcggaagaga gcacttacct ggaaactgct tatttgattc
     3421 tttacggtga attgcctagc aaatctgagc tcgcggaatg gattggaaac ataaccattc
     3481 attccatcat tcacgagaat ataaaaaaac atatagacgg cttccatcat gatgcccatc
     3541 cgatggggat cttaatcagc acggttgcag cgctgtccac tttttatccc gacgccaaga
     3601 aaatcttcga tgctaaatcc cgtcgcgagc aaacctatcg attgatcagc aagatgccga
     3661 ctctggcggc cttcgcttac cgccacagcc tgggcttgcc ttactcgtat cccgacaacg
     3721 atctgagtta caccggcaat ttcctcaata tgcttttcaa aatgaccgag ctcaaataca
     3781 agccgaaccc ggtgttggag aaagcgctcg acgttctttt tatcctgcac gccgatcacg
     3841 agcagaattg cagcacgaat gccatgcgcg gggtgtcgag ctcgcaagtc gacccatatt
     3901 cttcggtagc ggctgccgcc gccgcattat atggaccgct tcatggcggc gccaatgagg
     3961 cagttttacg catgctgatg ggaatcggct cgaaacaaaa ggttccggag ttcataaaaa
     4021 aggttaaagg cggcgaaggc cggctgatgg gatttggtca ccgggtatat aaaaattacg
     4081 atccccgcgc aaaaatcatc aagaacattg ccgatcaggt tttcgaggtt aaaggacgta
     4141 atcctctttt ggatattgcc cttgaactcg aacgcatcgc cctcgaagac gactacttta
     4201 taaaacgaaa actctaccct aacgtcgatt tttattccgg cctcatttat cagtcgatgg
     4261 gtctgcccat ggacatgttt cccgtgctat tcgcgattcc acgcacttcc ggctggatcg
     4321 cgcagtggga agagatgttg acagatcccg atcagaagtt ggcccgtcca aagcaggtct
     4381 atttaggcgc ggataagcgc gattacgtgg cgatggatcg acgaagctaa cgggcaactc
     4441 tgacgccgta gagattctaa cctttgccgt agcgcgcgat cgacaggagc ctcgcctcat
     4501 ctaagctcgt gaacgacggc tgtcgctctc tgcatctgac gttcaggtta cttgtcctcc
     4561 gcagcagggc cgacaatcgc atcccgccga attggccaat tacacgaaga cgatttctcc
     4621 gcggggagta gccttgaagc aactcgagat cagctctttg atcgttgcgc cgagggactt
     4681 acgtgattga ttgtgtttga ggttataact tccgcgggtg gtgaggtaca cttcgcattc
     4741 attggcgaac gttaccgttt gttcgacgga gaagggagat aaatgctcgt ggcaaaagca
     4801 aagtcatcca aacgaccgat ccatttctcg atgctgcgga gctatcaaac cgcggatctg
     4861 atcacacttg ccaacggcgc ggcgggaatg gcggcaattc tgtcgatgat gagctatctg
     4921 caatcacctg agaactggcg cgtgtacctg gcgctatcgc ttattcccgt ggcatttatc
     4981 ttcgatattc tggacggcaa gttagcccgc cggcagcaca agaattctgt attcggtcag
     5041 gaactggatt ctttggcaga catcgtc
//
LOCUS       DASUTG010000639         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2024882, whole genome shotgun sequence.
ACCESSION   DASUTG010000639 DASUTG010000000
VERSION     DASUTG010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024882"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1296)
                     /locus_tag="VF089_14725"
     CDS             complement(208..1296)
                     /locus_tag="VF089_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX6175261.1"
                     /translation="MSLLSFESYSLSLNGIIFAFSAALIWLAGTFLEKYADIISDRTG
                     LGQAFTGVVLLSTATSLPEVATTITAVALLNNPTLAVYNLLGGVALQTAILAIADRAK
                     HRTGPLTYFRPRFVLLIQGVGLLMLLQLTIAGITAHGLPTVFSISAWTIVLFLVYLGT
                     LYLTYRYRGQPRWTPTKRDDMPKEPPPEAKSNTESRTAYKERTAGRSTLSLWLRFCGA
                     SLAVLVAGWAVAQSADVLAKQTGLGDAFLGATLLALATSLPEVSTTVAASRNGHYSMA
                     ISNIFGSNGFCVALLFLAELLYRGGTILGQLQESVVFVTAIGAIMTSIYLFGMLERQD
                     RTVLGIGWDSAAAVVVYIGGMAVLYFIT"
     gene            complement(1312..>2210)
                     /locus_tag="VF089_14730"
     CDS             complement(1312..>2210)
                     /locus_tag="VF089_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6175262.1"
                     /translation="GAGHYEFGCLPCHGGPDLPQPVIAQQMTPHPPYLPPEISKWDAD
                     ELFYIVKHGVKFTGMPAWPALQRDDEVWAVVAFLLRLPRLTSQEYKELARSRNPGAAM
                     ENLSTPKEAPPAISESCARCHGLDGAGRGLSTFPILAGQRPTYLYASLLAFARGERHS
                     GTMIPLATGLSADDMREIARYYANLPRRSSSSARPDLAPAIERGRELAASGIPSKRVP
                     ACAACHGPGSTPRNPIYPNLAGQHAEYLALQLELFKKDIRGGTPYAHIMSSVAARLTP
                     EQISDVALYYSSLFTATEPAAQ"
BASE COUNT          482 a          616 c          687 g          425 t
ORIGIN      
        1 gctgcggaag gatttgcctc gcacttcctg tccaatcgca actgcggaat ccgggttcga
       61 accgtcagtc ataactgttc gtgtgcacat caaatacaca acccgcccag gcgggtcgca
      121 aattttacat ggcagcgatc tgatcgagat gatttcgtta cacgaaaaag gaggagatgt
      181 gttagtcgtg gccgtggggg cgaggctcta ggttataaaa tagagcaccg ccatgcctcc
      241 aatgtaaaca acaaccgcag ccgcggagtc ccagccgata ccgagcacag tccggtcctg
      301 gcgttcgagc atgccgaaga ggtatatgct cgtcatgatg gcgccaatgg cggtaacaaa
      361 gacgaccgat tcctggagct gtcccagaat agtgccgccg cgatagagca actcagccaa
      421 gaacaacagc gcgacgcaaa agccattgct gccgaaaata ttggagatgg ccatactgta
      481 gtgaccgttg cgcgaggcgg caacagtcgt gctcacttca ggtagagagg tagcaagcgc
      541 cagtaacgtc gcgcctaaga acgcatcccc caagccggtt tgtttagcaa gaacatcggc
      601 ggattgggca accgcccagc cggcgacgag cactgcgaga cttgcgccgc agaaccgcag
      661 ccacaggctt aacgtcgagc gtccggctgt tcgctccttg tacgcggtgc ggctctctgt
      721 attgcttttg gcttccggcg gcggctcctt cggcatgtcg tcgcgttttg tcggcgtcca
      781 gcgcggctgg ccgcgatagc ggtaggtcag gtatagtgtt cctaaataaa cgagaaacaa
      841 aacaatggtc catgcgctga tggagaagac cgtcggcaaa ccatgggcgg tgatgcctgc
      901 aatagttagc tgcaacagca tcagcagccc gacaccctgg atcagtagaa caaagcgcgg
      961 cctgaagtag gtcagaggcc cagtacgatg cttcgctcga tctgcgatag cgagaatggc
     1021 agtttgaagc gcgacgccgc cgagcaggtt gtaaacggca agcgtgggat tattcagaag
     1081 ggcgactgcg gtaatcgtcg tcgctacctc gggcaacgat gtggctgtcg agagcagcac
     1141 gactccggtg aaagcctgtc cgagtccggt gcggtctgag attatatccg cgtacttttc
     1201 aagaaaagtt ccggcaagcc agatcagcgc agcggaaaat gcgaagatga ttccgtttaa
     1261 cgataacgag tatgattcaa aagacagcaa tgacatcggc gcccgcgctg ttcactgcgc
     1321 cgcaggttcc gtggcggtga acaacgagct atagtaaagc gccacatcgc tgatctgttc
     1381 cggcgttagc cgggccgcaa ccgagctcat aatgtgagcg taaggcgtac cgcctcggat
     1441 gtctttcttg aacaactcga gctggagcgc gagatattct gcatgctgcc cggcgagatt
     1501 tggatagatg ggatttcgcg gtgtggagcc ggggccgtga caggcagcgc aggcgggaac
     1561 gcgtttgctc ggaatgccgg atgccgcaag ttcccgtccg cgttcgattg ccggcgcgag
     1621 gtccggccgt gcgcttgacg acgaccggcg cggcagattg gcatagtagc gcgcgatttc
     1681 gcgcatgtca tcggcgctca ggcccgtggc aaggggtatc atcgttccgc tgtggcgctc
     1741 gccgcgagcg aaagcaagaa gagaggcata gagatacgtc ggccgttggc ccgcaagtat
     1801 ggggaaagtg ctcagtcccc gtccggcgcc gtccagaccg tgacagcgcg cgcagctttc
     1861 gctgattgcc gggggtgctt ctttcggggt cgagagattc tccatggctg cgcctgggtt
     1921 tctgctgcgc gcaagctcct tgtactcctg cgacgtgagc ctcggaagcc tcagcagaaa
     1981 cgcgactact gcccatactt cgtcgtcgcg ctgcagcgcc ggccaagccg gcatgccggt
     2041 gaacttgacg ccgtgcttga cgatataaaa aagctcatcg gcgtcccact tggaaatctc
     2101 cggcggcaga tagggtggat gcggcgtcat ctgctgcgcg atcacaggct gcgggagatc
     2161 cggcccgcca tggcagggga ggcaaccgaa ctcgtagtgc cccgcgccct
//
LOCUS       DASUTG010000640         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2175426, whole genome shotgun sequence.
ACCESSION   DASUTG010000640 DASUTG010000000
VERSION     DASUTG010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175426"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF089_14735"
     CDS             complement(<1..673)
                     /locus_tag="VF089_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175263.1"
                     /translation="MTEPTLESAHAFGMSKQRIREVAQETDLLWNFACSLRQPLIFLF
                     RRRVLIDGDPGHLQVSALTWDMSIQDHHLFLSSGMNLHGRDCEVPTLGVKWHRFAQFV
                     YLPMWKFASDPGVNAPFSSVTQWTWEHLEFKGRALSVSKRDAYMRYVDLPRRAQRPFE
                     LAANIDRDDSTGDLESLRKMGWKLVNPHRVARTPSIYQNYIKRSRAEFCCPKPVHREL
                     KTGWFS"
     gene            701..889
                     /locus_tag="VF089_14740"
     CDS             701..889
                     /locus_tag="VF089_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175264.1"
                     /translation="MICEAVPMEAAEENIYESLVSHWVAGGLQDLEPKDVVSESTQAE
                     GILQKAAPVAATLRIPRQ"
     gene            complement(1118..1978)
                     /locus_tag="VF089_14745"
     CDS             complement(1118..1978)
                     /locus_tag="VF089_14745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175265.1"
                     /translation="MEQLLPDHLMKLWERVERKELLAEQFHLQQEQGIAEYRKIWTDA
                     LLLKGHRELKDSLIDELARYFKCHDLAEIQRRCCCAVDDLAKEWRDKVDPTDRRSVEN
                     FYNETEGEIYELMWWHTLSDDLSPLSYVTALQLAQRQGCHSCLDFGAGVGSGSILFAR
                     HGLKVGLADISSPLLAFSKWRFSQRDLPGDFFDLKINALPPESFDLVTAMDVFEHLAN
                     PVEVVEHLSDALKPGGFLFGRFGDEENEERPLHVVDDFEPSFRRLRELGFIQVWQDGW
                     LWGHQVFQKR"
     gene            complement(2020..2733)
                     /locus_tag="VF089_14750"
     CDS             complement(2020..2733)
                     /locus_tag="VF089_14750"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX6175266.1"
                     /translation="MAASKKRLIINADDFGQSSGINAGVIAAHQSGPVTSASLMVRWP
                     AAAEAVDYTRKQRTLSLGLHVDLGEWMFSNGNWVGVYEVVPLDDVNSLNKEVARQLKT
                     FHELVGKQPTHMDSHQHVHLREPARTVFLNAATNLNIPLRHFSSVVRYCGDFYGQTAQ
                     GFPLPDAISMSSLISILRTVPYGITELGCHPGEANDLETMYRAERAQELNILLDPELP
                     TMINSLGIELCSFADLPES"
     gene            complement(2751..3362)
                     /locus_tag="VF089_14755"
     CDS             complement(2751..3362)
                     /locus_tag="VF089_14755"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6175267.1"
                     /translation="MQSFFDDLWEPGDPWDLQASEFEQRRCSRLLEILSARRYGKALE
                     IGCATGVFTRLLTRVADRLVALDVSQNAIERAKASWVGPDSTEFRVANIIEYDPRAEG
                     PWDLVIMSDMIYFLGWLYPFFDIAWLAMELFAATSTHGQLLLANCYGGPGDHLMQPAL
                     IRTYRDLFLNVGYQVNTEDIFRGSKNGVDIDVLITLFEKKAKV"
     gene            complement(3451..>3696)
                     /locus_tag="VF089_14760"
     CDS             complement(3451..>3696)
                     /locus_tag="VF089_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175268.1"
                     /translation="SERSACYLAAGRPVITQDTGFGSVLPTGEGLFAFNTMDEIISAL
                     EDIQSNYPRHSRAASDIAQEFFRAEHVLKKVNDDLGL"
BASE COUNT          903 a          981 c          933 g          879 t
ORIGIN      
        1 cgctaaacca accagtcttg agttccctat ggacaggctt agggcagcaa aattccgcac
       61 gggatcgctt gatgtaattc tgataaatcg aaggagtcct tgcgacccgg tgcgggttta
      121 cgagcttcca gcccattttt ctcagtgact cgagatcgcc ggtcgaatcg tcgcgatcaa
      181 tattggcggc taactcaaac gggcgctgtg ctcgtctggg caaatcaaca taccgcatat
      241 atgcatcacg tttggaaaca cttagagctc gccctttaaa ctctaaatgt tcccacgtcc
      301 actgggtcac cgaactaaag ggcgcattga cgccggggtc agacgcgaat ttccacatcg
      361 gcaggtatac gaattgagcg aatcggtgcc attttacacc tagagtgggg acttcgcagt
      421 cgcgaccgtg cagattcatt ccactgctca ggaacaggtg atgatcctga attgacatgt
      481 cccaggtgag tgcggaaacc tgtaagtgtc cgggatctcc gtcgatcaga acgcgccgcc
      541 tgaataggaa aatcagcggc tggcgtaaac tacaggcaaa attccacagc aaatctgtct
      601 cttgggcgac ttctctaata cgctgcttgc tcatgccgaa agcgtgcgcc gactcgagcg
      661 ttggttccgt cacatgttga tcgtatagga gcaatgcaca atgatctgtg aagccgtacc
      721 gatggaagcg gccgaagaaa atatttatga gtcgcttgtc tcgcactggg ttgccggagg
      781 attgcaagac ctcgagccaa aagacgtcgt gtccgagagc acgcaggccg aagggatact
      841 gcagaaagca gctccagtgg ccgccacctt gcggataccc cgccagtgaa ttattcgcta
      901 gcacgatacg taggcgaggc aatattcctc cgggtcacac aaactaaatc tcacaactga
      961 cattgtgacg atgcggggtg tgttacgatt tctatgagcc gctgcttgaa ctcaccatgc
     1021 gagcaggaga agaatctaag aagttggacc ttacttgtgc gtagaataca tgccttcacc
     1081 tcggatcact atcgatagtg cgatagtgag acatggatta cctcttttga aatacctggt
     1141 gtccccataa ccagccatcc tgccaaacct gaataaagcc gagctcgcgc aggcgtcgga
     1201 agctaggctc gaaatcgtca acaacatgaa gtggacgttc ctcgttctct tcatcaccga
     1261 aacggccaaa gaggaaacct ccaggtttca acgcgtctga taggtgttcg accacttcca
     1321 ccggattcgc cagatgctcg aaaacatcca tcgccgtcac caagtcaaac gattcgggtg
     1381 gcagggcgtt tattttcaag tcgaaaaaat cgccaggcag atctcgttgc gaaaaacgcc
     1441 atttactgaa tgccagcaat ggggaactta tgtcggccaa tccaactttc aatccgtgac
     1501 gagcaaacag aatgcttccc gagcctactc ccgcgccgaa atccaagcag ctatgacagc
     1561 cctgccgttg agcaagctgc aatgcggtga cgtaggatag tggcgagagg tcgtccgaca
     1621 gggtatgcca ccacattaac tcataaattt ccccttcggt ttcattgtag aagttttcca
     1681 ccgatcttcg atccgtcgga tcgactttat cgcgccattc cttggcaaga tcatcgacgg
     1741 cgcagcagca gcggcgttga atttcagcca aatcgtggca tttgaaatac cgggcaagtt
     1801 catcgattag actgtctttt aactcccgat gtcctttcag aagcaaagca tctgtccata
     1861 tttttctgta ctcggcaatc ccttgttctt gctggagatg aaactgctcc gccaagagct
     1921 ctttccgttc gactcgctcc cataatttca ttaagtgatc gggcaacagc tgctccatac
     1981 gtattcttcc tcccacaaag tcaagtgtgc atcctgggct tagctctccg gtaggtcggc
     2041 gaacgagcaa agctcgatac ccaaagaatt tatcatcgtt ggaagttcgg gatcgagcag
     2101 gatattgagt tcttgtgcgc gctcagcgcg atacattgtc tcgagatcat tcgcttctcc
     2161 tggatggcag ccgagttccg ttataccgta cggcactgtc cttagaatac taatgaggct
     2221 gctcatgctg atcgcatctg gcagaggaaa tccctgcgcc gtctgcccgt aaaaatctcc
     2281 gcagtagcgt acgacggaac tgaaatgccg caaaggtatg ttgaggttag tagcagcatt
     2341 aagaaaaact gtacgtgcgg gttcgcgcaa atgcacatgc tggtgtgagt ccatatgcgt
     2401 cggctgcttg ccgacaagct cgtgaaaagt tttaagctgg cgagcaactt ctttgttgag
     2461 gctattcacg tcgtccaagg gaactacttc atacacacca acccagttgc cattactaaa
     2521 catccattcg cccaagtcga catgcagacc gagactgagc gtgcgttgct tgcgagtata
     2581 gtcaacggct tccgccgcgg caggccaacg caccatcagg ctagcgctag tcacgggccc
     2641 ggattgatga gccgcgatga cgcctgcatt gatgccggag ctttggccaa agtcatccgc
     2701 gttgattatc aaccgttttt ttgaagccgc catttagtaa gcgcctgtgc ttaaactttg
     2761 gctttttttt cgaataacgt gattagaacg tctatgtcta cgccattctt gctgccacgg
     2821 aatatgtcct cagtgttgac ctggtaacct acgttgagga aaagatcgcg atacgtacgg
     2881 atcaaagccg gttgcataag gtggtcgccg ggaccgccat agcagtttgc aagcaatagc
     2941 tgaccgtggg ttgacgttgc agcgaacagc tccattgcca gccaggcgat gtcaaaaaat
     3001 gggtagagcc aacccaagaa gtaaatcatg tcactcatga tcaccagatc ccaagggccc
     3061 tcggctcgcg gatcgtattc aataatattg gcgacccgga actctgtgga atccggaccg
     3121 acccagctgg cttttgcgcg ttcgatagcg ttttgagaga cgtcaagtgc caccaaccgg
     3181 tccgctactc gcgttagcag ccgggtgaag acgccagttg cgcagccaat ttcgagcgct
     3241 ttcccgtagc ggcgagcgga gagtatttcg agtaagcggg agcaccttct ctgctcgaat
     3301 tccgaggcct gaagatccca cgggtctccg ggctcccaga gatcgtcaaa gaaggattgc
     3361 attttgcgat ttctagattc tcggtcgctc atggtcgcct ttgcgtgtaa gcgtcagaag
     3421 attaactttt gagttgtttc tgagtcgtac tcacaatccc aaatcatcgt ttaccttctt
     3481 aagaacgtgc tccgccctga agaactcttg cgcaatgtcg ctggctgccc ggctgtgcct
     3541 tgggtaattc gactgaatgt cctcaagtgc ggaaatgatt tcgtccatgg tgttaaaggc
     3601 aaacaatccc tcaccagtgg gaagcacaga accaaacccc gtatcctggg tgatcacggg
     3661 gcggccagcg gcgagataac acgcgctcct ctcact
//
LOCUS       DASUTG010000641         8826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2025525, whole genome shotgun sequence.
ACCESSION   DASUTG010000641 DASUTG010000000
VERSION     DASUTG010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025525"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /locus_tag="VF089_14765"
     CDS             complement(<1..2458)
                     /locus_tag="VF089_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6175269.1"
                     /translation="MKDTRTPLNPAAANLERASGGLTNFPAVEKWDDWVEYDTAQWPR
                     KVEKHCRIIPTICFNCEAACGLVAIVEKDSHRIRRFEGNPEHPGSRGRNCAKGPATLN
                     QVTDPERIKYPLRRVGKRGEGQWERVSWDEVLDDIAARVRKALQEERRNEIMYHVGRP
                     GHELLYHQRILHSWGIDGHNSHTNVCSAGARAGYAFWSGIDRPSPDHANARFMLLLSS
                     HLETGHYFNPHAQRIIEGKERGAKICVIDTRLSNTASKADYWLSSWPGSEAAILLALC
                     NVLLQEDLFDHDFVRRWVNWEEYLRQEHPDKPQTFLSFVSVLKQIYGAYTPEFAAKEA
                     GVDPQVLVEVAHEIARAGSAFSSHVWRNAAAGNLGGWQVARALQFLVVLVGAVGTPGG
                     TAPNATNKYIPAPPLMPPPAKVWNELLLPREYPLAFFEMSFLLPHFIKEGRGKLAMYF
                     TRVYNPVWTNPDGMSWIEMLTDESKIERHACLTPTWSETAWFADYVLPMGHASERHDL
                     MSQETQAARWIGFRQPVLRVALEKEGKKFDLTYQAHAEAGLGEVWEEDEFWIELSWRV
                     DPDGSLGIRKYFESPYESGKKMTVDDYYRWIFENSVPGLPAAAAEQGLKPLEYMRKFG
                     AFLIEDEVYNVHETGLKPQDMEGATVDPATSIVTKKGVALGVEVDGKACAGFPTPSRK
                     LEFYSKTLKDWSWPEYAIPGYIKSHVHPDNIDFNAGEMLLLPTFRLPTLIHTRSGNAK
                     WLYEISHRNPIWLNPKDAQVLDVKTGDLLKINTEIGYFIDKAWVTEGIKPGVIACSHH
                     LGRWRLQENSGGERWSTALVD"
     gene            complement(2471..3994)
                     /locus_tag="VF089_14770"
     CDS             complement(2471..3994)
                     /locus_tag="VF089_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6175270.1"
                     /translation="MRYGFVIDQNRCIGCHACTVACKEEHNIAVGVNRTWVKYVEKGV
                     YPETRRHFAVLRCNHCDDAPCIEICPTVALFRRRDGIVDFDNERCIGCKSCMQACPYD
                     ALYIDPDRNTAAKCNFDASRVEMGYKPACEVVCPTQAILSGDLDDPQSDISRRVAMDK
                     VTVRKPEKGTKPKLFYAGVEGDLLTPAMMEPQSSHFWAEKPPGEDLYALSSGRADRPV
                     PGGAREVYDVPHMIPWGKKIATYLWTKSIAAGVLLLSALFLNMGFQHDDLILNFFSPI
                     ISLICLTATMLLLVFDLKKPSRFFFLLTKPNLNSWLTLGGYVLMIFGALTALWLGQRV
                     LTGAVSNVVVWAAALFAIASAGYSAFLFAQARGRDFWQSPLLFWHLLVQAIIAGAAML
                     TLVGVFAGVTVPMFYWLANLLVISLLVSVGMIFAELFLKHGADDAVRAGELLTKGALR
                     KPFWMLVIGLGTILPVLLILLPIGSVFPNVVAAVLALFGLWMYEHLWIKAGQALPLS"
     gene            complement(4152..5018)
                     /locus_tag="VF089_14775"
     CDS             complement(4152..5018)
                     /locus_tag="VF089_14775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6175271.1"
                     /translation="MLPIFFGILTSVCFAMASLLAQRGYHLAAAPWGAWITIAGNTVF
                     LLSAHFLLDPFAPIFVDDNLIFVAVGLLVPGVTRVLSFRGIRTMGSSVTSTIINTTPM
                     FSTVLAILLLGERPGPWVIAGVLVTVCGLITVSWTGATSSYRKIELIFPFLCALIFAC
                     KDVTVRWGLGDGGGQPILAAGIAALTSTVEIFVITRYIHGEKFCLPTANAARWFVWSG
                     IFTGGSFLCMYVALSMERVTIIAPLINSYAVFVLILTPFMARQIESVTQRKVAGAAMV
                     VAGIFMVSLGKH"
     gene            5096..6160
                     /locus_tag="VF089_14780"
     CDS             5096..6160
                     /locus_tag="VF089_14780"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6175272.1"
                     /translation="MHPTNLASIRRRLLGWFETNKRDLPWRRTLDPYAIWISETMLQQ
                     TQVKTVVPYYENFLKAFPTIAALDRAPLRHVRRVWSGLGYYRRAENLKKSASELMRRH
                     DGTMPQDYANLRALPGIGDYTAGAILSIAFQKTYPAIDGNARRVLGRLFNIAEAKQLR
                     AVAERLISKSNPGNFNQAIMELGATICLPAKPLCLLCPLTAHCWAHSGSGTSKKLAPQ
                     KKLTSRNVTWPLAIVRRGEKILLRRRGKTGLLAGMWEFPGGEKPSSYGVLTTLRRHLR
                     EIGPISGVPRGIGEIRHSITYRRICAPIFLFDLAVNDTIDLPASDWRWVAPAALDRYP
                     TSSMTVKAVRVLASYEKAAC"
     gene            6141..6899
                     /gene="mtgA"
                     /locus_tag="VF089_14785"
     CDS             6141..6899
                     /gene="mtgA"
                     /locus_tag="VF089_14785"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX6175273.1"
                     /translation="MKKLLAKSLLISAIIASLVYFYLALPNVSLLKKRNPQTSALMEL
                     RDQENREKKLPVSRQQIWVPYGRISDHLKKAVLVSEDASFFSHNGIDIDELKEAFKRD
                     LETLSFKRGGSTITMQLAKNLYLTPAKNPLRKMKEIMIAWQLERALSKTRIFEIYLNI
                     VEWGRNIYGAEAGSLHYFGKSAANLSPVEAATLVALLPSPRKTREKSILQRRNLALAR
                     LAKVGHISEEEYHRIKQIPLFHKAKKDAPQLLQD"
     gene            7061..7267
                     /locus_tag="VF089_14790"
     CDS             7061..7267
                     /locus_tag="VF089_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175274.1"
                     /translation="MEKGTRKLDTTLGELIAAASEVAFEYSDNDRDAYKLAQAALIEI
                     LRKASHADSLEDFGELAPKKTYLH"
     gene            7730..8695
                     /gene="msrP"
                     /locus_tag="VF089_14795"
     CDS             7730..8695
                     /gene="msrP"
                     /locus_tag="VF089_14795"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX6175275.1"
                     /translation="MLIRKSEDIKSSEITPKELYLNRRQFMISTSAAAVTAGAALAGF
                     GVFNGPSDVVAAEPIANVKKSSYSTSEKQNSLKDITNYNNFYELGPDKEDPAANAKYF
                     TPTRPWTVAVEGEVKKPKTYDIDALTKLSPLEERVYRLRCVEAWSMVIPWIGFPLNAL
                     IKLAEPTGNAKFIQFVTLHDPKRMPGQKLPIMEWPYVEGLRMDEAMHPLTILSVGLYG
                     QVLPAQNGAPIRVVVPWKYGFKSIKSIVKIRFVEQQPPASWNMMQPQEYGFYSNVNPE
                     VDHPRWTQATERRIGEFLRRKTLMFNGYGDQVAQLYSGMDLKKFY"
BASE COUNT         2170 a         2467 c         2306 g         1883 t
ORIGIN      
        1 gatcgactaa ggctgtcgac cagcgttcac cgccggaatt ttcttgcaat cgccagcgac
       61 cgagatgatg agagcacgcg atcacaccgg gtttgatccc ttcggtcacc caggccttgt
      121 cgatgaaata gccgatctca gtattgatct taagaagatc gccggttttg acgtcgagaa
      181 cttgcgcgtc tttgggattt agccagatcg gatttcgatg agaaatttcg tacagccatt
      241 tggcgttacc ggaccgggta tgaatcaagg ttggcaagcg gaaggtcggc aacagcaaca
      301 tttcaccggc attgaagtct atattatcgg ggtgaacgtg gcttttgatg tagcccggga
      361 ttgcatattc cggccagctc caatctttta atgtttttga atagaactcc aatttgcgcg
      421 aaggcgtcgg gaaaccggcg catgccttgc catcgacttc cacgcccaga gcaacgccct
      481 tcttggtgac gatgcttgtg gccggatcga ctgtggctcc ttccatgtct tgcggcttga
      541 ggcctgtctc atgaacatta taaacctcgt cttcgatcaa gaaggcgccg aacttgcgca
      601 tgtactcaag tggcttcagt ccctgttctg cggcggccgc cggaagaccg gggacgctgt
      661 tctcaaatat ccaacgatag taatcgtcca ccgtcatctt tttgccagac tcgtacggtg
      721 attcgaagta ttttcgaatg ccgagagaac catcgggatc aacccgccac gagagctcga
      781 tccaaaattc gtcttcttcc caaacctcac cgagaccagc ttcggcgtgg gcctgatagg
      841 taagatcaaa ctttttccct tctttttcca aggcgactct taacaccggt tggcgaaagc
      901 caatccagcg cgcggcctgg gtctcctggg acatcaagtc atggcgttcg gatgcatggc
      961 ccatcggcag gacgtaatcg gcaaaccagg cagtctcgct ccaggttggt gtcagacacg
     1021 cgtgacgttc gatcttactt tcatcggtca gcatttcgat ccagctcata ccgtcgggat
     1081 tggtccagac agggttgtaa acgcgggtga aatacatcgc cagcttgccg cggccttctt
     1141 tgatgaaatg aggcaggaga aaactcattt cgaagaacgc cagcggatac tcccgtggca
     1201 atagcagctc gttccacact tttgccggcg gcggcatcaa cggcggtgcc ggtatgtact
     1261 tgttggttgc atttggcgcc gtgccacccg gcgtgccgac agccccgacg agcaccacca
     1321 ggaactgcag cgcgcgcgcc acctgccaac caccgagatt tccggcagcg gcattgcgcc
     1381 acacatgaga cgaaaaagcc gaccccgcgc gagcaatctc atgggccact tcgacgagaa
     1441 cctggggatc cacaccggct tctttagcgg cgaactccgg cgtgtaagcg ccatagatct
     1501 gcttcaacac ggatacgaac gagaggaatg tctgcggctt gtcgggatgc tcctggcgca
     1561 aatactcctc ccagttaacc catctcctga caaaatcatg gtcgaagaga tcttcctgca
     1621 agagaacatt gcacagagcg agaagaatcg ccgcctcgct acccggccat gatgacagcc
     1681 agtagtctgc tttggatgcc gtattggata agcgtgtatc gatcacgcaa atcttggcgc
     1741 cacgctcctt tccttcgatg atgcgctgcg cgtgcggatt aaagtaatgt ccggtttcga
     1801 gatgagagct gagcaacagc atgaaccggg cattggcatg atccggcgaa ggccggtcga
     1861 tacccgacca gaaggcatag cccgcgcgag cgccggcgga gcagacgttg gtgtgactgt
     1921 tatggccgtc gataccccag gaatgcaata tccgctgatg gtagagcaac tcgtgccccg
     1981 gcctgccgac gtggtacatg atctcgttgc ggcgctcttc ttgaagcgct ttgcgcactc
     2041 gcgcggcgat gtcatcgagg acctcgtccc atgagacccg ttcccattgg ccttcgccgc
     2101 gtttgccaac ccgcctgagc ggatacttaa tgcgttccgg atcagtcact tgattaagag
     2161 ttgccgggcc tttggcgcag ttgcgtcctc tgctgccggg atgttcagga ttgccttcga
     2221 aacgtcgaat acgatggctg tctttctcga cgatcgccac cagaccgcaa gctgcctcac
     2281 agttgaaaca gatggtcgga atgatacggc agtgtttctc gactttgcgc ggccactgcg
     2341 cggtgtcata ttccacccaa tcgtcccatt tctcgacagc gggaaaattg gtcagccccc
     2401 cgctcgcccg ttcgaggttg gctgcagccg ggttaagtgg agtacgagtg tccttcattt
     2461 gaaatccttc ttaacttaac ggtaacgcct ggccggcctt gatccacaga tgctcgtaca
     2521 tccacaagcc aaacagcgcc aaaaccgcag cgacaacatt cggaaatacg gaacctatcg
     2581 gcagtaagat tagcagcacc ggcaggatag tgccgagccc gattacgagc atccagaatg
     2641 gttttcgcag cgctcctttg gtcaacaatt cgccggcccg gaccgcgtcg tcggcgccat
     2701 gcttcaaaaa cagctcggca aagatcatcc cgacactgac gagcagcgag atcacgagca
     2761 agttagccag ccagtaaaac atcggcaccg tgactccggc aaaaacgccc accaaggtaa
     2821 gcatcgctgc gccggctatg atcgcctgca ccagcaggtg ccaaaacaac agcggacttt
     2881 gccagaaatc tcttcctcgg gcttgggcga aaagaaatgc tgaatatccc gctgacgcga
     2941 tcgcaaacaa cgccgccgcc cagaccacca cattggagac agcgccggtg agaactcttt
     3001 gccccagcca taacgctgtc agagctccga aaatcatcag gacatagccg cccagcgtta
     3061 accaggagtt taaattcggt ttagtcagga ggaaaaaaaa ccgcgacggt ttcttgagat
     3121 cgaaaaccag cagcaacatc gttgcagtaa gacaaatcaa cgaaatgatc gggctaaaga
     3181 aattcagaat caaatcgtca tgttggaagc ccatgttcaa aaacaacgct gaaagcagca
     3241 gcacacccgc ggcgatggat ttggtccaga gataggtcgc gatttttttc ccccagggaa
     3301 tcatgtgcgg aacatcatag acctcacggg caccgccagg aaccggccta tccgctctgc
     3361 cactgcttag agcgtaaagg tcttcgccgg gcggcttctc cgcccagaaa tggcttgatt
     3421 ggggttccat cattgccggt gtgagcaaat caccctcaac gccggcataa aagagttttg
     3481 gtttggtccc tttctccggc ttgcggaccg tgactttgtc catggcaacg cggcggctga
     3541 tgtcgctctg cggatcgtcg agatctcctg agaggatcgc ttgcgtcggg cacaccactt
     3601 cgcaagcggg cttatagccc atctctaccc gcgatgcatc gaagttacac ttagccgccg
     3661 tgttgcgatc ggggtcgata taaagcgcat cgtatgggca tgcttgcatg caggatttac
     3721 agccgatgca gcgctcgttg tcgaagtcga caatgccgtc acgccggcga aagagtgcca
     3781 cggtcgggca aatctcgatg cagggcgcgt cgtcacagtg attgcagcga agcacggcaa
     3841 agtggcgccg ggtttccgga taaacccctt tttcgacata cttcacccag gtccgattca
     3901 ctccaacggc aatgttgtgt tcctccttgc atgctaccgt gcaggcgtga caaccgatac
     3961 aacggttctg atcgatgaca aatccgtagc gcattgagaa cctttcgtct taaagctggc
     4021 aaccagcgag gataatcctt aggtcatagc aaagggcggc acgaaactcc aaccaggact
     4081 cagcggaaac ccatgcacga taatttctgt caagaatcgc agacgccaaa caagtgaagc
     4141 tgcattggct cctaatgttt tcccagggat accatgaaga ttcctgccac caccatagcg
     4201 gcgccggcaa ctttgcgctg ggtcacgctc tcgatttgcc gcgccatgaa cggcgtcaag
     4261 atcagtacga ataccgcgta actattaatc agcggagcta taatcgtgac ccgctccatg
     4321 ctgagggcga cgtacataca caggaaagac ccgccggtaa aaatccccga ccagacaaac
     4381 cagcgcgcgg cattggccgt aggcaggcaa aatttttcgc cgtgaatata tcgcgtgatt
     4441 acaaagattt cgactgtcga ggttagcgcg gcgatccctg ccgccaggat cggctgcccg
     4501 ccgccgtcgc cgaggcccca acgcaccgtg acgtctttac aagcgaagat caacgcgcag
     4561 agaaatggaa agatcaattc gattttacga taggagctcg tcgctccggt ccacgagacg
     4621 gtgatcaggc cacagacggt taccaaaact ccggcgatca cccacgggcc cggccgctcc
     4681 cccagaagaa gtatcgccag aaccgtggaa aacatcggtg tcgtattgat gatggtggat
     4741 gtaaccgagc tgcccatggt tctaataccg cggaaactca gcacccgggt tactcccgga
     4801 accaacaaac ccacggcgac aaaaatgaga ttgtcgtcaa cgaagatcgg cgcgaagggg
     4861 tcaagtaaga aatgcgccga taacaaaaac acggtattgc cggcaatagt gatccaggcg
     4921 ccccaaggag ccgcggccag atgatagccg cgctgtgcca agagactggc catggcaaag
     4981 caaaccgaag tcaaaatccc gaagaagatt ggcagcacgg agctttataa gtttccttcg
     5041 caagcgattt cgtctagagt gccccttata accgaatttt ttcacaatcg cattcatgca
     5101 tccaaccaat ctcgcctcca tccgccgcag actgctcggt tggttcgaaa cgaacaagag
     5161 agatcttccc tggcgcagga cgttggatcc ctacgccatc tggatttcgg aaaccatgct
     5221 acagcagacg caggtcaaga cggttgttcc ctattacgaa aattttctta aggcgttccc
     5281 gacgatcgcg gcattggatc gcgccccgct acgacacgtc aggcgtgtct ggtcggggct
     5341 agggtactat cgccgagccg agaatctaaa aaaatccgcg tccgagctga tgcgccggca
     5401 tgacggaacg atgccgcaag actacgcgaa tttgcgcgcg ctgccgggaa tcggcgatta
     5461 caccgcgggc gccattctca gcatcgcctt tcagaaaacc tacccggcta ttgacggcaa
     5521 tgcgcgacgg gtactcggtc gtttgttcaa catcgctgag gcgaaacagt tgcgcgcagt
     5581 cgcagagcgg ctcatctcaa aatcaaaccc gggtaacttt aaccaagcga tcatggagct
     5641 cggcgcaacc atttgtctgc cggcaaaacc gctttgttta ctttgcccgc tcactgccca
     5701 ctgctgggcg cacagcggaa gcggaacatc caagaagctg gcgccccaga aaaaactgac
     5761 gtctagaaac gtcacctggc ctcttgcgat tgttcgccgc ggtgaaaaaa ttctattacg
     5821 gcggcgcggc aaaacaggct tgctcgccgg aatgtgggag tttcccggcg gcgagaagcc
     5881 gtcgagctat ggggtcctga caacgctacg gcgccatctg cgggaaatcg gacctatttc
     5941 aggtgtccct cgcgggatcg gcgaaattcg ccacagcatt acttaccgtc gcatttgcgc
     6001 gccgatcttt cttttcgatt tagccgtcaa tgatactatc gacttgcctg cttccgattg
     6061 gcgctgggta gccccggccg cgctggaccg ctatcccaca tcctccatga ctgtcaaagc
     6121 ggtccgggtt ttagcaagct atgaaaaagc tgcttgctaa gagcctgctc atctcggcga
     6181 taatcgcgtc tctggtttat ttttatcttg ccttgcccaa tgtttcgctc ttgaagaaaa
     6241 gaaatcctca gacctctgcg cttatggaac tacgcgacca ggagaacaga gaaaagaaat
     6301 tgccggtttc gcggcagcaa atttgggtgc cgtacgggcg gatttcagat catctaaaga
     6361 aagctgttct ggtcagcgaa gatgcctctt tctttagtca taacggaatc gatatcgacg
     6421 agctcaagga agcattcaag agagatcttg aaacacttag cttcaaacgc ggcggcagca
     6481 cgatcacaat gcagttagcg aagaatctgt atttgacccc ggcaaaaaat cccctgcgga
     6541 aaatgaaaga gatcatgatc gcctggcagc ttgagcgggc tctttcaaaa actcggattt
     6601 ttgagattta tctaaacatc gtcgaatggg gacgcaatat ttacggcgcc gaagcaggat
     6661 cgcttcacta ctttggcaaa tccgccgcca atttatctcc ggtcgaagcc gcgactctgg
     6721 tcgcgctgct gccaagcccg cgaaaaactc gagaaaaaag tattcttcaa aggcgaaatc
     6781 tcgctttagc aagattggcc aaggtaggcc acatcagtga ggaggaatac catcggatca
     6841 agcaaatccc gctcttccac aaagccaaga aagacgctcc ccagctgctg caggattagg
     6901 atcgaggggg cgcgtaccac ctaaatttct ccggattcgc agtacatcct ttttcttgcc
     6961 aaatcgtcta actgttgggg acaacctcgg gcgaaagaaa gcctcaacct catcgcagaa
     7021 cgcaatcaac acctttcgcc tgacaaagaa aggagaacga atggaaaagg gcacgagaaa
     7081 gctggacact accctgggcg agctcattgc cgccgccagc gaagttgctt ttgaatactc
     7141 cgataacgat agagatgcct acaagttagc tcaggcagca ttgatcgaaa ttctcaggaa
     7201 agcttcccat gctgacagct tagaggattt tggtgaattg gcgccaaaaa aaacctatct
     7261 tcactgatcc caccatcgaa catgggggcg gaatccgccc ccatatggct aaattttcgc
     7321 tttgtagcaa tcgcgggaac tttaagccac cactgatcgt tattcattca gattaggaag
     7381 cgcaacccaa ctcgaactta agtcatctaa tcacaagcgc gcaagaacgt gcggtgacga
     7441 ggagcggttg gaggattttc gcaaaattct ttttgagatt cgccggtttc tagcaggctg
     7501 ctgaaaaaga cccatatgct tcgttgccgc tcaaccgcct cgcctctgga tctttttgag
     7561 cagcctgcaa tagccttttt tcagcaccct gctagttcga aatttgacag cgtcgcggca
     7621 tctcggggta taccgacaat cgtcttgatc gacaagatag gcgcaatccg gtatagccat
     7681 atcgctaagg ggcttagaaa aaaccatcgc aagaaaagaa agagggccca tgctgattag
     7741 aaaatcagag gatatcaagt cctccgagat tactcccaag gagctgtatc tcaaccgtcg
     7801 gcaatttatg atttcgacct cggctgccgc tgtgacggca ggcgcggctc tcgctgggtt
     7861 cggggttttc aacgggccga gcgacgttgt tgccgccgaa ccgattgcca acgtcaagaa
     7921 gagctcctac agcaccagcg agaagcagaa ctcgctcaaa gacatcacca attataataa
     7981 tttctacgag ctcgggcccg acaaagaaga tcccgcggca aacgccaagt acttcactcc
     8041 gactcggccc tggacggtgg cggtggaggg tgaggtcaaa aagcccaaaa cctacgacat
     8101 cgatgctttg accaagctga gtcctctgga ggaacgagtc tatcgactgc gctgtgtcga
     8161 agcttggtcc atggtaatcc cgtggatcgg ctttccttta aacgcgctca tcaaactcgc
     8221 cgaaccgacc ggcaacgcca agtttatcca gtttgtgacc ttgcacgacc cgaagcggat
     8281 gcccggccaa aaattgccga tcatggagtg gccttatgtc gaaggcttgc gcatggatga
     8341 agccatgcac ccgttgacga tcttatcagt gggcttgtac ggccaggtct tgccggcgca
     8401 gaacggcgcc ccgattcgcg tggtggtgcc atggaaatac ggtttcaaaa gcattaaatc
     8461 catcgtcaag atccgtttcg tcgaacaaca accgccggcg tcgtggaaca tgatgcagcc
     8521 gcaggagtac ggtttttact ccaacgtcaa tccggaagtc gaccatccac ggtggaccca
     8581 agcgacggag cggcgcatcg gcgaatttct ccggcgcaaa acgctcatgt tcaatggtta
     8641 cggtgatcag gttgcgcagc tttacagcgg catggacctg aagaaattct actaggagta
     8701 aagccgaatt cgcgattcag caagcgcgcc aggaaggcgg agatcacaac tgctcgcgta
     8761 tcgggaattg ataaattgca ctggctgtct tcttccgagg cctgaattct gctggtaacc
     8821 cagatc
//
LOCUS       DASUTG010000642         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1135485, whole genome shotgun sequence.
ACCESSION   DASUTG010000642 DASUTG010000000
VERSION     DASUTG010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135485"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="VF089_14800"
     CDS             complement(<1..1945)
                     /locus_tag="VF089_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HEX6175276.1"
                     /translation="MRNRSHSKLSAHQAGETFQEVLSRNIARRSFLKGALISAPLILA
                     GPGALQRHFAQAEEDDGLRFKPINLDNQDRVLVAEGYDAHVLIRWGDPVFRDSPSFNP
                     LNQSKLTQEHQFGYNCDYVAYLPLPNDPNRALLWVNHEYTNEAIMFPNYDEANPSQEQ
                     VDVALAAHGASVIEIRKNGRDHWEVVTGSPYNRRITGETHMQITGPAAGDDMMKVSYD
                     QTGRRVRGMLNNCGGGTTPWGTVLTCEENFNQYFANRAGLPDTDERKTNHTRYGVGSS
                     VSGLPVGRSERGWELYHDRFDLAKDPNEPFRFGWVVEIDPYNPHFIPKKRTALGRLKH
                     EAATTVVAPDGRIAVYTGDDERFEYMYKFVSREKYSDKNRAANFNLLDEGTLYVAKFN
                     DDGTGQWLPLVYGQGTLVAPTFTSQADVLINTRFAADAVGGTKMDRPEDIETNPVNGK
                     LYCAMTNNTNRGTGTNPTANAANPRTGNRHGHIIEVTEDSNDPTSLNFAWEIFLLCGN
                     PATQPVDSPTPTLLAEATYFGGFDHSKVSPISSPDNIAFDRKGNLWIATDGQPTTFGM
                     NDGVFAVPVEGEERGFLRQFLSGVPGGEVASLHFGARDHALFVTIQHPGEPGTGPNSS
                     GSTFETPTSVWPEGNTPPRPSVVV"
BASE COUNT          427 a          545 c          626 g          517 t
ORIGIN      
        1 cgacaacgac gctcggacgg ggtggagtat taccttcggg ccatacgctt gtcggcgtct
       61 cgaaggtgct gccgctgctg ttcgggccag ttccgggctc gccgggatgt tggatggtca
      121 cgaacagggc gtggtcgcgc gcgccgaaat gaaggctggc aacctcgcct cctggtacgc
      181 cgctcaaaaa ctggcggaga aacccgcgct cctcgccttc gacgggaacc gcgaatacgc
      241 cgtcattcat gccgaacgtg gtaggctgtc cgtctgtagc tatccagaga ttgcccttgc
      301 gatcgaaggc gatgttatcc ggtgacgaaa tcggactgac tttgctatga tcgaagccac
      361 cgaaataagt ggcttcggca agcagcgtgg gtgtcgggct atcgaccggc tgagtcgcag
      421 gattaccgca gaggagaaat atctcccagg caaaattcaa ggatgtagga tcgttgctgt
      481 cctcggtgac ttcgatgata tggccgtggc gattaccggt tcttggattg gcagcattag
      541 ccgtggggtt cgtgccggtg ccgcggttgg tgttgttagt catggcgcag tagagtttgc
      601 cattgaccgg gtttgtttcg atgtcttctg gccggtccat cttggttcct ccgacggcat
      661 cggcggcgaa ccgagtgttg atcagaacat cggcttgtga cgtgaaggtg ggtgcgacca
      721 gggttccttg accataaact aacggcaacc attgaccggt gccatcgtcg ttgaatttag
      781 cgacataaag cgttccctca tcgagcaggt tgaagttggc cgctcggttt ttatcgctat
      841 atttctcgcg gctgacaaac ttgtacatat attcaaatcg ttcgtcgtcg ccggtgtaga
      901 cggcgatgcg accgtcgggc gcaacaacag tggtcgcggc ttcgtgcttg agccgtccta
      961 gggcagtgcg tttcttcgga ataaaatggg gattgtaggg atcgatctcg acgacccagc
     1021 cgaatcggaa cggttcattg ggatctttcg caagatcgaa gcggtcgtgg taaagttccc
     1081 aaccacgttc gctgcgacca accggcaagc cgctcacgct ggagccgacg ccgtagcggg
     1141 tgtgattcgt ctttcgctca tctgtgtccg ggagaccggc gcggtttgcg aaatactgat
     1201 tgaagttttc ctcgcaggtg agaaccgtgc cccacggcgt cgtgccaccg ccgcagttgt
     1261 tcagcatgcc tcggactcgc ctgccggtct ggtcgtagga gactttcatc atgtcgtcgc
     1321 ctgcggctgg tccggtgatt tgcatgtgag tttcaccggt tatgcggcgg ttgtaaggcg
     1381 atcccgtaac tacctcccaa tgatctcgcc catttttcct gatttcgatg acgcttgctc
     1441 cgtgcgctgc cagagcaaca tcgacctgtt cctgactggg atttgcctca tcatagttgg
     1501 ggaacataat cgcttcattg gtgtactcat ggttgaccca caacaatgcc cggttaggat
     1561 cattcggcag tggaaggtaa gcgacgtagt cgcagttata gccaaactgg tgttcttggg
     1621 tcagtttgct ctgattgagc ggattaaagc ttggggaatc cctgaacacc ggatctcccc
     1681 agcgaataag cacatgggca tcgtatccct cggcaacgag cacacggtcc tgattgtcca
     1741 gattgatcgg tttgaatctc aagccgtcat cttcctcggc ttgggcaaaa tgccgctgca
     1801 aggcgccagg ccctgcgaga ataagcggcg cggaaatcag tgctcctttt aaaaacgagc
     1861 ggcgcgcaat attccggctg agcacttctt gaaaagtttc tccggcctga tgagccgata
     1921 gcttagaatg cgaacgattt ctcatagatt cctccaaatg tgagatgcgg cgatactagc
     1981 catccgatcg ttacggcgat gtaacgagac aacgaagtat cggtgaagga atggttacga
     2041 agagatcgtt tggattccta gctcggcaaa ttctggtgtt tgcggatagt tcggggggcg
     2101 cagcgcctat gcttt
//
LOCUS       DASUTG010000643         4414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2175999, whole genome shotgun sequence.
ACCESSION   DASUTG010000643 DASUTG010000000
VERSION     DASUTG010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175999"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="grdC"
                     /locus_tag="VF089_14805"
     CDS             complement(<1..1320)
                     /gene="grdC"
                     /locus_tag="VF089_14805"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HEX6175277.1"
                     /translation="MSNYLPVIKAASSVLIHTPGLTRYGSKPQREIAANPARLADLNR
                     SLRSFQQAQGYLPNQVFVGQQAPEKMSLFSRPWFGEQAMRTEERKAFGSIIDERLFLA
                     LIALADPFKHVTLLDRFWEDIGAPLQQSIVCRSLATSAPSLTDANRLKTMIAKGEGMP
                     LFAGSETEPVGWIANGHHEDESLSATVLLENLCGKVSAALSLQEMFDGNPGLKKQDVD
                     FVLGCSEEAVGDRYQRGGGNLAKAIAEFSGCDMASGFDIKDFCAAPIPAMVTAAALVQ
                     SGVAENIVVVGGSSLPKLGMKFLYHLEKNIPVLEDMQAAVAVWVGRDDGKSPIMNLAG
                     VGRHPVSAGASLDQQLKSLVVDPLNRLELGVPAIDKFVTELHNPEITLPANGGDVAER
                     NYRMLGAVLVKAGKLAKSDLATFMKEKGLPGYAPTQGHIASALAYVPH"
     gene            complement(1565..2869)
                     /locus_tag="VF089_14810"
     CDS             complement(1565..2869)
                     /locus_tag="VF089_14810"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX6175278.1"
                     /translation="MRLELADYPVKEIRLGRTFEYHSGTLVIDRTALEGMILSDRRIA
                     EASLGVVCPGDEVRITGIRDIVEPRVKASGNGQVFPGILGPVEAVGAGRTHRLSGMAV
                     IATAAYEGTVRAGLGVQRSALLDMWGAGAAASKFGGLLHLVLIMKLRDGLSELDAHTA
                     IQTAEFQVARRLAETTIGVEPANVEVFDLGQRHADLPNIVLVQGCLTDDHHVHSGVSY
                     YGLPIRESLSTVVHPNELLDCAMAVNATRGIGYFPITWDWQNHPLVLGLYRDHGRRLN
                     FIGMILQRIRYETFHGKEVIAQNAAQLTATLGAQGALVTWLGSGNAFVDVMLTVRACE
                     RRGIATVLVTYEYGGKDGIDSPLLFYVPEADAVVSSGTRDRWLDLPEAQRLVGPYDRV
                     QILSYPGAPVAQAQGKLTLDARDMVIGGVDNWGKESWICNAY"
     gene            complement(2876..4279)
                     /locus_tag="VF089_14815"
     CDS             complement(2876..4279)
                     /locus_tag="VF089_14815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3155..3157),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX6175279.1"
                     /translation="MNTQKMIRCFHSRITAKEDYSMNTIVHVVNQFFAGLGGEEKAGM
                     SVGVIEDAAGAARGLQSQLGAELKVACTIYYGDNYFHEHNEEAVKVILEAVRSHGAQA
                     LVAGPAFNSGRYGMSCIEICHAVSTTLKVPCVTAMHEENPAIGTYRDLADARVFCLPT
                     TETAAGMTEALKALAKFVSRLARGETIGPALQEGYFSRGIRRLEKTDRTGADRAIDML
                     LKKINHEPFTTEVPMETWDDIAPAPPLDDLKARNIAVITTSGVVPWGNPDRFKTYRNT
                     HWRKYNIAELKELEPGKWEAVHGGYNVAYMNKNPHYGVPLDALRSLEADGVIGAGKLY
                     PAYYVIPGNQGSPAVMRRVGQEIAADLKKDNVEGVLFVATUGTCSRSGAVMSKELEKA
                     GLPVALVSAMYPVAEQVRANRIVKGVSIPHPCGDPSLPQDLDARLRREIVQTALKALE
                     SEVTGPTVFTPNMAVSG"
BASE COUNT          946 a         1335 c         1194 g          939 t
ORIGIN      
        1 atgcggcaca tacgccagcg ccgaggcgat atggccctgg gttggcgcat aaccaggcaa
       61 tcctttttct ttcatgaagg tcgcaagatc actctttgcc agcttgcccg ccttgaccag
      121 gaccgcgccg agcatccggt agtttcgctc cgcaacgtcg ccgccgttgg cgggcaaggt
      181 aatctcgggg ttgtgcagtt cggtgacgaa cttatcgatc gccggcacgc ccagttcgag
      241 ccggttcagc gggtcgacaa ccaatgattt gagctgctgg tccagtgaag cgccggccga
      301 taccggatgg cgtccgacgc ctgccagatt catgatcggg cttttgccgt catcccgacc
      361 gacccatact gcaactgccg cctgcatgtc ttcgagcacc gggatgtttt tctcaagatg
      421 gtaaagaaac ttcataccaa gcttgggcag agacgatccc ccgacaacta cgatgttttc
      481 agcgacacca ctttgcacga gcgcggcggc cgtgaccatc gccggaatcg gcgcggcaca
      541 aaaatcttta atgtcgaaac cggaagccat gtcacaaccg gaaaactcag caatcgcttt
      601 agctaaattg ccgccgccgc gctggtaacg atcgccgacc gcctcctccg aacagcccag
      661 cacaaagtcg acgtcttgct tcttgaggcc cggatttccg tcaaacatct cctgtagcga
      721 caaagccgcc gagaccttgc cacagaggtt ttccaacaac accgtcgcag ataaggactc
      781 atcttcgtgg tgaccattgg cgatccaacc gaccggttcg gtctcgctgc cagcaaacag
      841 cggcatgccc tcgcctttcg caatcatcgt cttcagtctg tttgcatcag tgagactcgg
      901 agcactcgtt gcaagcgatc tgcaaacgat cgactgctgc agaggcgcgc cgatgtcctc
      961 ccagaatcga tcgagcaacg taacatgttt gaacggatcc gccagggcga tcagcgcgag
     1021 aaacaatctt tcgtcgatga tgctgccgaa ggctttccgt tcctcagtgc gcatcgcctg
     1081 ctctccgaac cacggccggc tgaacaggga cattttctca ggcgcttgct ggccgacgaa
     1141 aacctgattg ggcaagtagc cctgcgcttg ctgaaaggac ctgagacttc ggttcaaatc
     1201 cgcaagacgc gccggattgg cggcaatttc ccgttgcggt tttgacccat accgtgtcag
     1261 acccggagtg tgaatcagga cgctcgaggc tgcttttatt actggcaagt aattcgacat
     1321 ggctgttact tcaggatgta taaactaatg acgctgacga tcaagaggcg acccgcgagc
     1381 agcgcgggta taagatcact gtcaccaacg actgttccgg gtgaatgaag aatgtgtctt
     1441 gcaggctgct caaagacctc agaggtaagg cgcgcgaagt tcgatgagct gaggcgtacg
     1501 cttcagtaca tcgaagcgag gcgctcgagc gcgacaacac ctatgagtct ttttcagcag
     1561 cttgctagta ggcattgcag atccaggact cctttcccca attgtcgaca ccgccgatca
     1621 ccatgtcgcg cgcgtctaac gttagttttc cttgtgcctg cgccacgggg gcgccaggat
     1681 aactgagaat ctgcacccga tcgtacgggc cgaccagacg ttgagcttcg ggcagatcca
     1741 accagcggtc gcgggtccca ctgcttacca ccgcatcggc ttccggaaca tagaacagca
     1801 gcggcgaatc gatgccgtcc ttgccgccgt attcataagt taccaggacg gtggcgatgc
     1861 cgcgccgttc acatgcgcgt accgtcagca tcacgtcgac gaaggcgttc ccggaaccca
     1921 gccacgtcac gagcgctccc tgggcgccga gcgttgccgt caattgcgcc gcattttgcg
     1981 caatcacttc cttgccatga aaggtctcgt accgaatgcg ttgcaaaatc atgccaatga
     2041 aattcaagcg ccggccgtga tcacggtaca atccgagcac caaaggatga ttttgccagt
     2101 cccaggtaat cggaaaataa ccgataccgc gggtggcatt gacggccatg gcgcaatcga
     2161 gcaactcgtt gggatgaacc acagtcgaga gcgattctcg tatcggcaga ccgtagtagc
     2221 tcacacccga gtgaacatgg tgatcgtcgg ttaaacagcc ttgaaccaag acgatgttcg
     2281 gcaaatccgc atgcctttga ccgagatcga aaacctctac gttcgcgggc tcgacgccga
     2341 tggtcgtctc ggcaagacgg cgggcaactt gaaactcagc ggtttgaatc gccgtgtgcg
     2401 catcgagctc cgaaaggcca tcgcgcagct tcatgatcaa taccagatgc aaaaggccgc
     2461 cgaatttcga cgcagccgca cccgctcccc acatatcgag cagagcgctg cgctgcacgc
     2521 ccagtccggc tctaacggtt ccctcgtacg ccgccgtcgc aattacagcc ataccggaca
     2581 agcgatgcgt gcgaccggca ccgaccgctt ccaccggtcc gagtatccct ggaaacacct
     2641 ggccgttgcc gcttgctttc acccggggct caacgatgtc gcggataccg gtgatacgca
     2701 cttcgtcgcc aggacaaacc acacccaagg atgcttcagc gattcgccta tcgctaagga
     2761 tcattccttc cagcgctgtt cgatcgatca caagcgttcc ggagtggtat tcgaacgtcc
     2821 ggcccagacg gatctctttg accggatagt cagccagctc gagtcgcatg cttgtttacc
     2881 cgctcaccgc catgttcggg gtaaatactg tcggtcccgt gacttcggat tcgagcgctt
     2941 tcaatgcggt ttgaacgatc tctcgccgca gtcgcgcatc gagatcctgc ggcaggctcg
     3001 gatcgccgca cgggtgaggt atgctaacgc ccttgacgat ccgattggct cgcacctgct
     3061 cggccacagg atacatcgcg cttaccaggg caaccggaag tcccgccttc tcgagttctt
     3121 tgctcatcac agcgccgctg cgactacaag tccctcacgt ggcgacaaag agcacgccct
     3181 caacattgtc cttcttcaga tcggcggcga tttcctgccc gacgcggcgc atcaccgccg
     3241 gggagccttg attgcctgga ataacgtaat aggcggggta cagtttgccg gcgccgatca
     3301 ccccgtcggc ttctaacgac cggagcgcat cgagcggcac gccgtagtgc ggattcttgt
     3361 tcatgtaggc gacattgtag ccgccgtgca ccgcttccca ttttcccggt tcgagctctt
     3421 taagctcggc gatgttgtat ttacgccaat gggtgttgcg ataggttttg aaccgatccg
     3481 gattgcccca gggtacgacg ccgctggtgg taatgacggc aatgttgcgc gcttttagat
     3541 catcgagagg cggagccggc gcaatatcat cccaggtctc catcggcact tcggtagtaa
     3601 agggctcatg gttgatcttt ttgagcaaca tatcgatcgc ccgatcggcc cccgtccgat
     3661 cggttttttc cagacgccgg ataccacggc tgaagtaacc ttcctgcaac gccggaccaa
     3721 tggtctcgcc gcgagccagc cgagaaacaa atttcgccag cgccttgagc gcttcggtca
     3781 taccggcggc ggtctccgtt gtgggtaaac agaaaacgcg cgcatcggcc aggtcccgat
     3841 aggtgccgat agcggggttt tcttcgtgca ttgcggtgac acagggaact ttcaaggtcg
     3901 tggaaacggc gtgacaaatc tcgatgcagc tcatgccgta gcgcccggaa ttaaacgctg
     3961 gcccggcgac taatgcttgc gccccgtgag agcgaactgc ttcgaggatc actttgacgg
     4021 cttcttcatt gtgctcatga aagtagttgt cgccataata gatcgtgcaa gcaaccttaa
     4081 gttcagcgcc gagctgcgac tgcagccctc gcgccgcgcc ggcggcgtct tcgatcacgc
     4141 cgacggacat gcccgccttc tcttcgccgc ccaaaccggc aaagaactga ttaacgacat
     4201 ggacgattgt gttcatcgag taatcctcct tcgcagtgat gcgtgaatga aagcaccgta
     4261 tcatcttttg cgtattcatc gatagctcgc ggtggcggcc ggaaggctcg ctccttcgct
     4321 ccagtttcgg aattctccag tctagtagtg tactgaaaac tctcttgcaa gatgctcaca
     4381 aagacctcag aggcaagcaa ctgtcttaaa agtc
//
LOCUS       DASUTG010000644         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_693253, whole genome shotgun sequence.
ACCESSION   DASUTG010000644 DASUTG010000000
VERSION     DASUTG010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693253"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..775)
                     /locus_tag="VF089_14820"
     CDS             complement(449..775)
                     /locus_tag="VF089_14820"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX6175280.1"
                     /translation="MLMHIKQSWQRFKAGLPGRRFQQQFRLRRGFRSGAARKALFITA
                     GILLAAAGVFLLFVPGPGIITLLIGAVLIAQQSSLAARAFDRIEIRLRKFIKENFHPT
                     RRGERN"
     gene            complement(1183..1368)
                     /locus_tag="VF089_14825"
     CDS             complement(1183..1368)
                     /locus_tag="VF089_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175281.1"
                     /translation="MLGILFFFARTLASLLGGFGLFLSLLFFLADRQNKKKRLWERSP
                     RHTWVNENPPLEVSRHG"
     gene            complement(1584..>2783)
                     /locus_tag="VF089_14830"
     CDS             complement(1584..>2783)
                     /locus_tag="VF089_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6175282.1"
                     /translation="VRELRRDGLFNHVVCAGESEPAEISFASLTCDRGITVPTSDTAA
                     DEPAFIVYTSGTTGKPKGIVHAHRWIVALGDLNRYRLPPQQGDVIMATGEWSFISALG
                     HNLLFPLRNGVTGAILSARATPENILRYIEEYRVTVLHSVATVYRRLLAMPAFEQQHD
                     LSSLRCAHSTGEALRAATYNEWKVRVGSELYEHYGVSEYQLVIGHGIRHPVKPGSVGK
                     LLPGVPAAILNDDFKPVENGDLGWFAISTADPGLFLGYYKDPERTEAVIRNGWYFTGD
                     LAYQDDDGYFFIAGRRDDCFKSRGIFISPTEIENALQKHPAIIEAAVVAQPDPEIGNK
                     IRAVIVLAPGQAPSDSMTQSVRDLLRAQIAPYKIPHIIEFAHNLPKSAVGKILRTALV
                     HKTSDSR"
BASE COUNT          641 a          743 c          766 g          633 t
ORIGIN      
        1 tgccaaagat tgatccgaca aattcacgac gtgtcaacgg cgtttgcgtt taacagcgcc
       61 tgctgttcag gttttgctca gtcgaatgga caggtgggta ctagtttggc tctcgctaac
      121 gcgggaaagt ttcaatcggg atccttcgtc ctaacactgt cctcgtgtcc acacttcgta
      181 caacgagcgc gtaagctggt cttgatcgcc tgcggcgtaa tctcctccgt gtcaagtatg
      241 agtttcatcg atccttcgcc gcatgccgga cacttggagt tttcctcgac cattgggttt
      301 tccgaaaata ccttagggag agattagatg ggagcgatca agtcgtcgaa cgtgcatcca
      361 cagcgtgcat cgatgtcata gttgtccccg tccggatttg aatcacttct gcccgctctc
      421 gagatcggga gtccaaaaag cataagtctt aattccgctc gccccggcgc gttggatgaa
      481 agttctcctt tatgaatttc cgtaaacgga tctcgatccg atcgaaggcc cgagcagcca
      541 aagaggactg ctgcgcgatg agcactgcac cgatgagaag agtgatgatg ccaggtccgg
      601 ggacaaagag aagaaagacc ccggctgctg ccagcaagat gccggcggtg atgaataatg
      661 cttttcgagc ggcaccgctg cgaaaccctc ggcgtagccg gaactgttgc tggaatcggc
      721 ggccgggcaa gcctgctttg aaacgttgcc agctttgctt tatgtgcatc aacatggacg
      781 atcccgcgct tcacgagcag gctgccgaaa aagactcata gacttcgtgg cgctcagttg
      841 cctcgcgtga atgaattcgt gctcgtggta cgtgttcgtg ctcgttcgaa attctcgacc
      901 acgttttcac ggacacgatc acgaggattc cgcattgcct ctgaagactt tttcagcggc
      961 ctatagtcag agtttttcag caagctgcta gaacatcaaa tctcgcattg ggcgatcgat
     1021 gacgagaagg atgctgaaaa aagcctcttg caggctgctc aaaaagatcc agaggcgaga
     1081 cgcgcgaaaa atcgaccagc ggaggcgtac tcacactaca tcgcagcaag gcgattgagc
     1141 ggcaacgaag catatgggcc tttttcagca acctgctagt aatcatccat gacggctaac
     1201 ttccagcggc gggttttcat tcacccacgt atgtctcggt gatctctccc aaagccgctt
     1261 cttcttattt tgtcgatcag caagaaaaaa caacagcgac agaaacaatc caaagccgcc
     1321 gagcagggat gccaacgtac gagcgaagaa aaatagtatt cccagcatat gatttcacct
     1381 tctgcagcgc ctgagcttgc tcgcgcctgt gtcagagtag tcctgaactt ctcacattaa
     1441 ttctaacact cacggagagc agaaaaaggt aggggcgacg aattggacgt aacgctatat
     1501 ccaaatagtt gaaccgtgaa attcacgcgt tcaaggttca agccggctat ctcaccatga
     1561 aatatgagat cgttacgcgt ccgtcagcgg ctgtcacttg ttttgtgaac gagagctgtc
     1621 cgaagaatct ttcccacggc gcttttcggc aagttgtgag caaactcgat gatgtgcggg
     1681 attttatagg gggcaatttg tgctcgcagc aggtcgcgca ccgattgcgt catcgaatcg
     1741 gacggcgctt ggccgggcgc taaaacgatc acagctcgaa tcttgttgcc gatctcggga
     1801 tcaggctggg caacaaccgc ggcttcaatg atggccggat gtttctgcag agcattttcg
     1861 atctcggtcg gcgagatgaa gatgccgcgg ctcttgaaac agtcgtcacg ccggccggcg
     1921 atgaaaaaat aaccgtcgtc gtcttgatag gcgaggtcgc cggtaaaata ccagccgttg
     1981 cgaataaccg cttccgttct ttccggatct ttatagtagc caagaaatag accgggatct
     2041 gccgtcgaga tcgcgaacca accgagatct ccgttctcta ccggtttaaa atcatcgttc
     2101 aggatcgcag ctgggacgcc cgggagtagt tttcctaccg aaccgggctt taccggatgc
     2161 ctgatgccat ggccgatcac gagctggtat tcggacacgc cgtagtgttc gtagagctcg
     2221 gagccgacac gaactttcca ctcgttgtaa gtcgctgccc gcaacgcttc tccagtcgaa
     2281 tgagcgcagc gtagactgct gagatcgtgc tgctgctcga acgccggcat ggcgagcagc
     2341 ctccggtaca cggtcgccac cgaatggagc accgtcactc tgtactcttc aatatagcgg
     2401 agaatgtttt ccggcgttgc gcgggccgac agaatggcgc cggtgactcc gtttcgcaac
     2461 ggaaaaagca gattgtggcc gagagcgctg atgaagctcc attccccggt ggccatgatc
     2521 acatcgcctt gttgcggcgg caatcggtag cggtttagat cgccaagggc gacgatccag
     2581 cggtgcgcgt gaacgatgcc tttgggcttg ccggtcgttc ccgacgtgta gacgatgaag
     2641 gccggttcat ccgcggctgt atcgctggtc ggtacggtga ttccgcgatc gcaggttagc
     2701 gaggcaaaag agatctcggc gggttccgac tcgccggcgc acacgacatg gttgaacaat
     2761 ccatcacggc gcagttcgcg cac
//
LOCUS       DASUTG010000645        10666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_990100, whole genome shotgun sequence.
ACCESSION   DASUTG010000645 DASUTG010000000
VERSION     DASUTG010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990100"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF089_14835"
     CDS             complement(<1..297)
                     /locus_tag="VF089_14835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035979.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6175283.1"
                     /translation="MNEIAHNQDTGFYTEDMTISVGPQHPSTHGVLRFVVKTDGEVIG
                     EAIPDVGYLHRSIEKIGEKVTWHGYVPYTDRADYLAAMFANQVFCMAAEKLSGTP"
     gene            complement(305..811)
                     /locus_tag="VF089_14840"
     CDS             complement(305..811)
                     /locus_tag="VF089_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6175284.1"
                     /translation="MQPAEIFSRLENQFSGKVSGFKADVPEPYLLVDAPAIVEVCLFL
                     RDDADLKFEVLSDQTAVDWPKDEKIQLVYHLYSYSSRHQIVLKVDLPRDNPKIATVEN
                     VWKVANWFEREIFDLFGVVFEGHSDLRRILLPEDWEGYPLRKDYIEQEEYDGISTQRA
                     PLVEKLLG"
     gene            complement(813..1325)
                     /gene="nuoB"
                     /locus_tag="VF089_14845"
     CDS             complement(813..1325)
                     /gene="nuoB"
                     /locus_tag="VF089_14845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX6175285.1"
                     /translation="MKPLINSLPETVFTTKVDDLLNWGRASSQWYMLFGLACCAIELM
                     QTGGPRADLDRFGAVPRATPRQSDLMIVAGTLTYKMAKRMKLLYDQMPDPKYVISMGS
                     CSNCGGLFQLAYSVCKGVDKIVPVDVYVPGCPPRPEALTEGLLRIQDKMMRERWLVKP
                     NSQTEAAVRA"
     gene            complement(1348..1734)
                     /locus_tag="VF089_14850"
     CDS             complement(1348..1734)
                     /locus_tag="VF089_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6175286.1"
                     /translation="MLFDFANVLVFTALGLGFVGVNLLIGRLLRPANPQARKLSTYEC
                     GEPSTGSAWVNFNIRFYLVALIFIIFEVEIAFIFPVAVVFRNLIESGEGLFAFIKIFI
                     FIGILFLGLIYSWVKGDLDWIKKVRA"
     gene            2492..3703
                     /locus_tag="VF089_14855"
     CDS             2492..3703
                     /locus_tag="VF089_14855"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175287.1"
                     /translation="MRIETERKVKRWRDQRWLLDQVIQARGIDWDQGRTGKILRNCGP
                     GVQSDLQEVCRRIQKFVDIPREFARAAIRREQQAREAEDAGHLAEARDHFYIAACFYT
                     NAMWAVYEDRNPRRISWQERKRACYDEFIRYAGRPIERVELPYQGKLIYALLHLPPGM
                     KAGEKVPCVMYIPGMDGVKEDNPATGDPFLERGIAVLAIDGPGQGQTREGGLTCTASN
                     YADAGRLACDYLVNRSEIDPNRLGILASSMGSYWAPRVVAEEKRFKACAVSGVCMEPG
                     QYAIFNMSSPTFKLNYMYMSGYNDEDAFDEFAKTLTLKGVTAKITCPYMVVAGEDDEH
                     CDMNFVYELMGEIPAPKLLYVFEGERHSIRNLRARSLLIDWLVDRLLNKPFKSEKIFV
                     ETSGEEIRSDW"
     gene            3782..4942
                     /gene="larC"
                     /locus_tag="VF089_14860"
     CDS             3782..4942
                     /gene="larC"
                     /locus_tag="VF089_14860"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX6175288.1"
                     /translation="MRVAYGDLIGGISGDMFVAALLDLGLSLEVLSRELRKIPTLKFE
                     LKVDKKTVGSIAATQFQVKAPRNEAPRSWKEIHQLIETSQLAHEIQETGIKIFHRLAE
                     VEGKIHGVPTEEIHFHEVGATDSIVDIVAAAIGFHELKIGAMHFSKVPLGRGVIQSGH
                     GPLPVPGPATLELLKGIPVQWTQLAGETVTPTGAAIMSSLGISFGEQPSMTVESIGYG
                     AGQKEWPDRPNLFRLVLGATVDWRQEEMLVIETNIDDMNPQFFEHVMERLFAAGAGDV
                     FFSPIQMKKNRPAVLVRIIAELHNRDRLAKILFEETTTIGLRYHSVSRIILNRTEATI
                     KTRFGDVKIKLWQEPSGRKRISPEYDELKRIAIEKKIPLNVLRDEIVKSFKE"
     gene            complement(5050..5775)
                     /gene="bioD"
                     /locus_tag="VF089_14865"
     CDS             complement(5050..5775)
                     /gene="bioD"
                     /locus_tag="VF089_14865"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX6175289.1"
                     /translation="MGNGVFITGTDTGVGKTFFACKLAALLRKSGYRVGVMKPAESGC
                     AEEGGKLYPQDAILLKEASASQDPLDKICPYQLAEPLAPSVAAQRQGITLEIDRLMDV
                     YEEISSDQDITLVEGAGGLMVPLLPSFTYADFARVLKLPTIVIAANRLGMINHLLLTL
                     EHARCKGLCVLGYVLNRLESGASLAADTNREALSTLTAAICAGELPYAAPSDNAAYGD
                     AESFAEQFDLRIIASVLRRRPVR"
     gene            complement(5775..7133)
                     /gene="bioA"
                     /locus_tag="VF089_14870"
     CDS             complement(5775..7133)
                     /gene="bioA"
                     /locus_tag="VF089_14870"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX6175290.1"
                     /translation="MTRDYDKLKQLDHSYLWHPFTQMQEWMGEDPCIISHGEGHYLID
                     VNGRRYFDGVSSLWCNVHGHRKKELDDAIRGQLDRISHSTFLGLSHVPGIELAQKLIE
                     VAPPGMQRVFYSDSGATAVEVALKMAVQYWQLKREAKRTLIATLAESYLGDTIGSMSM
                     GYSETFHRYHKSLLFPVLRITPPHVFRYYQKMSGHDALKAAIDEAERKISENKHRLAA
                     LVVEPLMQGAAGMWAQPVEYLQALDEICRRCEILLVLDEVATGFGRTGKMFACDHARV
                     RPDIVCLAKGITGGYLPLAATLTSEQIFAAFLGDYKDFKTFFHGHTYTGNPLGCAAAL
                     ANLDLFQRDNIIEDMQPRISYLHKRLAEEFLSLSHVSDVRQWGFMVGIELVEDRGSRR
                     SYPPEKRIGHKVIIEARKRNLMIRPLGDIVILMPPLSITDGELALLLDTVRESILAVT
                     GN"
     gene            complement(7146..8300)
                     /gene="bioF"
                     /locus_tag="VF089_14875"
     CDS             complement(7146..8300)
                     /gene="bioF"
                     /locus_tag="VF089_14875"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX6175291.1"
                     /translation="MMAGFIQDELQALKSASLFRRLRSVAGDQGPTLMVDGREVLNFS
                     SNNYLGLANHPALGKAAIDAIGRYGCGSGASRLISGNMTLHEELEAKLAELKGAEAAL
                     VFNSGFQANTGIIPTLVGDGDVVFSDALNHASIIDGCRLSRAKIVVYRHCDVEQLEYG
                     LKRAPRSSRKLIVTESLFSMDGDEAPLKQIVELSEKYDAMIMVDEAHATGVYEPDGAG
                     LVSQLGLAERVLVQMGTLGKALGGFGAYIAGSAMLRELLINRCRSFIFTTSLPPAVMA
                     MGIAALELIKHEPERRRVLRDNCARLRSGLTKLGYSPGDSRSQILPLLIGDANECMRL
                     SSRLLDRGVFAQGIRPPTVPPGTSRLRITLMATHTSDQIDRALEVFASVD"
     gene            complement(8396..9049)
                     /locus_tag="VF089_14880"
     CDS             complement(8396..9049)
                     /locus_tag="VF089_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717365.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX6175292.1"
                     /translation="MVIERAEKISASDFLPRSRSLESLRQAARSCKGCDLYLNATQTV
                     FGDGPSHASVMLVGEQPGDIEDQKGAPFVGPAGRMLDRALEDARISRDEVYVTNAVKH
                     FKWIWRGKRRLHQKPSVRQVVACRPWLEVEIEAVQPQILVCMGATAAQSVVGKPLAIM
                     KERGRFIDSVLGKLTFVTIHPSAILRQRDNDEREQEYRRFAAEMKLVKRKLQSLSAA"
     gene            9220..10581
                     /gene="radA"
                     /locus_tag="VF089_14885"
     CDS             9220..10581
                     /gene="radA"
                     /locus_tag="VF089_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX6175293.1"
                     /translation="MAKPKTFYACQSCGYQAPKWLGKCPDCNEWNTFAEERLERATSV
                     RGELSLGTKEDPAPIHEINTAEEGRLQCGIGEFDRVLGGGLVPGSVILIGGDPGIGKS
                     TLLLQAFAAISQKGLTCLYVSGEESPRQIKMRAERLGIVAPNLLVLSETSLERIIEHV
                     KNIQPGLLVIDSIQTIFSPTIPSAPGSIAQVRESSGSIIVLAKKTGLTTFLIGHVTKD
                     GAIAGPRLLEHMVDTVLYFEGERGHSFRVLRAVKNRFGSTNEIGVFEMKEAGLKEVSN
                     PSEIFLMERPLQVPGSAVVCSIEGTRPILIELQALVSRSFLAVPRRTTIGVDHNRVAL
                     LVAVLEKKMGAKLFDQDIFVNVAGGVHVDEPAVDLGIVAAIASSSRENVLDPKTVFFG
                     EVGLAGEIRGISQAETRIKEAGKLGFERCVLPASNSAQLKNIKHPKLSGVSSLNECWK
                     VMF"
BASE COUNT         2602 a         2937 c         2765 g         2362 t
ORIGIN      
        1 cggcgttcct gagagttttt ccgccgccat gcagaaaact tgattggcaa acatcgcggc
       61 caagtagtcg gcgcgatcgg tatacggcac gtagccgtgc caggtcacct tctcgcctat
      121 tttttcgata gaacgatgca aataacccac atccggaatc gcttcaccga tgacttcgcc
      181 gtcggttttc accacgaaac gcaacactcc atgcgtgctc gggtgttgcg ggcctacgct
      241 gatggtcatg tcttcggtgt aaaatccggt atcctgattg tgagcaatct cgttcatcgc
      301 aatcctatcc cagcagcttt tctaccaagg gagcgcgttg ggtactgatg ccgtcgtatt
      361 cttcctgctc gatgtagtcc ttgcgcaggg gatatccctc ccaatcttca ggcaagagta
      421 tccggcgcag atcactgtga ccttcaaaga caaccccgaa cagatcgaag atctcgcgct
      481 cgaaccaatt ggcaactttc caaacgttct caacagtcgc aattttcgga ttgtctcgag
      541 gaaggtcgac tttgagcaca atctggtgcc tactgctata agaataaaga tgataaacga
      601 gctggatttt ttcgtcttta ggccaatcca ctgccgtttg atccgaaagc acctcgaact
      661 tcaagtcagc atcatcacgg agaaagaggc agacctcgac aatcgctggt gcatcgacga
      721 gcagataagg ctccggcaca tcggccttga agccgctaac ctttcccgaa aattgatttt
      781 ccagccgcga aaaaatttca gcaggttgca tactatgccc gcaccgctgc ctccgtctgg
      841 ctattgggct ttaccagcca acgctcacgc atcatcttat cttgaatccg caacaaaccc
      901 tccgtcagcg cctctgggcg cggcggacat cccggcacat agacatcgac aggcacgatc
      961 ttatcaacgc ctttgcagac ggagtacgcg agctggaaca ggccgccaca gttcgaacag
     1021 ctacccatcg aaatgacata cttcggatcc ggcatctgat cgtaaagaag tttcatccgt
     1081 ttggccattt tatacgtcag cgttccggca acgatcatta gatccgattg gcggggggtc
     1141 gcccggggaa ccgcgccaaa tcgatctaaa tcggcccgcg gccccccggt ttgcattaat
     1201 tcaatggcgc aacacgctaa gccaaacagc atgtaccact gtgaagatgc ccgcccccag
     1261 ttaagtaagt catccacttt ggtggtaaaa acggtttcag gaagtgaatt gattaatggt
     1321 ttcatctaac cctccgaacg attgcggtta ggccctaacc ttcttgatcc agtcgagatc
     1381 acctttgacc caggaataaa tcaggcctag gaatagaatt ccgataaata tgaagatttt
     1441 aatgaatgca aataatccct cgccgctttc gatgagattg cggaatacga cggcaacggg
     1501 aaaaataaag gcaatttcca cctcgaaaat tataaaaatc agcgcaacta gataaaagcg
     1561 aatattgaaa tttacccagg cgcttcccgt cgagggctcg ccacactcat aagtcgacag
     1621 tttgcgggct tgaggatttg ccggccgaag caaacgcccg atcaacaggt tcactccgac
     1681 gaatccaagc cctaatgccg taaacactag gacattagca aaatcaaaca gcatcgtaag
     1741 tttctgtagt aattacttaa agacaggcaa caattcttat aaccataaaa gccatagaga
     1801 atcaacctta cgacaatcct gattgtcgct catctatttg ccattgctag cttttttgac
     1861 tagagtcgga ccgatcttaa gtttcgaacc atcacctgaa ccaacctttc cacagaaaag
     1921 ggagaccgcc gccccattct tatgaaccgg accatccgat tagataatca gccacatcca
     1981 attagatgat cagccacatc gtcctgatcg ttttctggtg gtcgggccag gaaatcacgc
     2041 acgcccgtcc gtgttgctta cagcgcgtta tcggcctgga ttgggttctc tggctgaccg
     2101 gcgaacaagg gattttccgc aagcatggct tggaatcgaa acagtatcta tgcgcagcga
     2161 cctaccgcag cgcaagcgtt tctaacgggc gagatccact ttggccattt tcaccggcgc
     2221 ttagatgacg gcttgggcgc agtgcgccga tacctcgggt gggaacgact ctaccttaat
     2281 aagtcttcac tctgatgatg gaggcaaaca tcgccaaggc agctgaccta gagagaaaag
     2341 aaactcggca tcattcggat cggttcggca agcgatctcg acgatcgctt cgtaaaagcc
     2401 gcgttaatgt ggcagccaac ttcgatcaag gacagaatcg cggctcgttg catgttttat
     2461 ccttggacct ttctaggcgt cgggaggcgg tatgagaatt gagactgaac gaaaagtgaa
     2521 acgctggcgc gaccagcgct ggctactcga ccaagtcatc caagctcgtg gcatcgactg
     2581 ggaccaagga cgtacgggta aaattctacg caattgcggt ccgggggttc aaagcgatct
     2641 ccaagaagtt tgccgaagaa ttcagaagtt cgttgacatc ccgcgcgagt tcgcccgcgc
     2701 cgcgatccgc cgtgaacagc aagcacgtga agccgaagac gctggacacc tggcggaagc
     2761 gcgcgaccat ttctacattg cggcgtgctt ctacaccaat gccatgtggg cggtctatga
     2821 agacagaaat ccgcggcgaa ttagctggca agaaagaaaa cgcgcttgtt atgacgaatt
     2881 cattaggtac gccggccgcc ccatcgagcg ggtagaactt ccctaccaag gcaaactcat
     2941 ctacgcactt ctgcacctgc ctcccgggat gaaagccggc gagaaagtcc cgtgcgtcat
     3001 gtacatcccg ggaatggatg gggtcaaaga ggacaatccc gcaaccggcg atccgtttct
     3061 tgaacgtggt atcgccgtcc tcgcaattga tggcccgggt cagggacaaa cgcgcgaagg
     3121 cgggctcacc tgtaccgcat cgaattacgc ggacgcgggt aggctcgcct gtgattacct
     3181 ggttaacaga tcggagattg atcccaatcg acttgggatt ctggcttcga gcatgggctc
     3241 ctattgggcg ccgcgggtgg tggccgaaga aaaacgtttc aaagcctgcg cggtaagcgg
     3301 tgtttgcatg gagccgggcc agtacgcgat cttcaacatg tcatcgccga cgtttaagct
     3361 taactacatg tacatgtccg gttataacga cgaggatgcc ttcgacgagt tcgccaagac
     3421 cctgaccttg aaaggcgtta cagcgaagat aacttgcccg tacatggtag ttgccggcga
     3481 ggacgacgag cattgcgaca tgaattttgt ctatgaactc atgggtgaaa ttcccgctcc
     3541 gaaacttctg tacgtattcg aaggagagcg gcactcgatc cgcaatctac gtgcgcgatc
     3601 cctgttgatc gattggttgg ttgatcggtt gctaaacaaa ccgttcaagt cggagaagat
     3661 ctttgtcgag accagcggtg aagagattcg ttctgactgg tagccgaggc atcccatgcg
     3721 ataaatacgg caattttccg atggcgtgaa cacaaatgac cgccttatct caggtaaatt
     3781 gatgcgagtt gcttatggtg atctgattgg cggaatcagt ggggacatgt tcgtcgccgc
     3841 ccttctggac ctgggactct cgcttgaagt tctcagccgt gagctgcgaa agatccccac
     3901 acttaagttc gaactcaaag ttgataagaa aactgtcggc tcgatcgctg cgacacagtt
     3961 tcaagtaaag gcgccgcgca atgaagcgcc acgctcctgg aaagagatcc accagctcat
     4021 cgagacaagt caactagccc acgagatcca agaaaccggc atcaagatct tccaccgtct
     4081 ggccgaagtc gagggaaaaa tccacggtgt gcctaccgaa gaaattcatt ttcacgaagt
     4141 gggcgccacc gattctattg tggatatcgt cgcagctgca atcggctttc acgagctcaa
     4201 gatcggcgcg atgcattttt ccaaagtccc gctcgggcgc ggtgtaatcc agagcggcca
     4261 tggtccgttg cctgtacccg gacctgcgac tctggagctc ctgaaaggca ttcccgttca
     4321 atggactcag ctcgcgggcg aaactgtcac tcctaccgga gccgcgatta tgtccagcct
     4381 aggaatcagc ttcggtgaac aaccgagcat gaccgtagaa agtatcggtt atggcgccgg
     4441 acaaaaagaa tggccggatc gtccgaacct atttcggctc gtcctcggcg cgaccgtcga
     4501 ttggcggcaa gaagagatgc tcgtcattga aaccaacatc gacgacatga atccgcagtt
     4561 tttcgagcat gtcatggaac ggctatttgc cgccggtgct ggagatgtct ttttctcgcc
     4621 gattcaaatg aagaaaaacc gtcccgccgt gcttgtgcga atcatcgccg aactgcacaa
     4681 tcgggacagg ctggcaaaga tactcttcga ggaaacaacc accattggcc tacgctatca
     4741 ttccgtgagc cgcataatcc tcaacagaac ggaggcaacg atcaaaacac gctttggcga
     4801 tgtgaagata aaactctggc aggaacctag cgggagaaag cgcatcagcc ctgaatatga
     4861 cgaactaaag cggatcgcca tcgagaaaaa aatcccgctt aacgtcctgc gggatgagat
     4921 cgtcaaaagc ttcaaggagt aaccactgtg accgctcgct aatatagaac tcctcgactc
     4981 atctacccat ctatgagagc gaatgagaac aaggtcagtt gattgatcga gcgcggcgtt
     5041 tgtagggcat cacctaactg gtcgccgtcg taatacggat gcaatgatcc gaagatcaaa
     5101 ttgctccgca aatgactccg cgtcaccgta tgcagcattg tccgacggcg ctgcataggg
     5161 caattccccc gcacaaatcg ctgcggtcag cgtcgagaga gcttcacggt tggtatccgc
     5221 cgcgagcgat gcaccgcttt ccagccggtt caaaacgtaa ccgagaacgc acaaaccttt
     5281 acagcgcgcg tgctcgagcg tcagcagcag atgattgatc atcccgagcc gattcgcggc
     5341 aattacaata gtaggaagct tgagaaccct ggcgaaatca gcgtaggtaa agctgggtaa
     5401 tagcgggacc atcagtccgc cggcgccttc cacaagagtt atgtcttggt ctgaactgat
     5461 ctcctcataa acatccatca atctgtcaat ctccaacgtg atgccttggc gttgtgccgc
     5521 cacactgggg gctagcggtt cagctagctg atacggacat attttatcca gcggatcttg
     5581 agaggcagat gcctctttta aaagaatagc gtcttgggga taaagcttac ccccttcttc
     5641 ggcgcagccg ctttccgccg gcttcatcac gccgacccgg tatccggact tacgcagaag
     5701 cgccgccagc ttacaggcga aaaaggtctt gccgacgccg gtgtccgtgc cggtaatgaa
     5761 aactccgttc cccatcagtt tcccgtcacg gcgagaatag actcacggac cgtatccagg
     5821 agtagcgcga gctcgccatc cgtaattgac agcggcggca tcaatatgac gatgtcgccc
     5881 agtggccgaa tcatcaggtt gcgtttgcgc gcttcgatga tcactttgtg gccgatgcgc
     5941 ttctccggcg gataactcct gcgactgcct ctatcctcaa ccagctcgat accgaccata
     6001 aacccccact gccgcacgtc gctcacgtgc gacaaagaga gaaattcctc tgcgagtcgc
     6061 ttatgaaggt atgagattct cggctgcata tcctcgatga tgttgtctct ctggaacaag
     6121 tcgagattcg cgagcgcagc cgcgcagccg agagggttgc ccgtataagt atggccgtga
     6181 aagaaagttt taaaatcctt gtaatcaccc aaaaacgcgg caaaaatttg ctcgctggtt
     6241 aatgtcgctg ccagcggcaa gtaaccgccg gtgattcctt tggccagaca gacgatatcg
     6301 ggccttactc tagcgtgatc gcatgcgaac atctttccgg tgcgaccaaa gcctgtcgcg
     6361 acctcatcga gaaccaataa gatttcacag cgccggcaga tttcatcgag agcttgcaaa
     6421 tattcaacgg gttgagccca catcccggcg gctccctgca taagcggctc tacgactaga
     6481 gcggctagcc tgtgtttatt ctcgctaatt ttcctttccg cttcatcgat agctgctttt
     6541 aacgcatcat gcccgctcat tttttgataa tagcgaaaga catgcggcgg cgtgattcgc
     6601 aggaccggaa acagcaggct tttgtgataa cgatgaaagg tttcagaata gcccatgctc
     6661 atcgagccga tggtatcgcc gaggtaggac tccgccaggg tcgcaatcag ggtgcgcttg
     6721 gcttctcgtt tcaactgcca gtactggacc gccattttga gagccacttc aaccgccgtg
     6781 gcgccgctgt ccgaatagaa aacccgctgc attcctggag gagccacttc gataagcttt
     6841 tgcgccaact caatgccggg cacatggcta aggccgagaa atgtggaatg ggagatgcgg
     6901 tcgagctggc cgcgtatcgc atcgtcgagc tcttttttgc gatggccgtg aacgttgcac
     6961 caaagcgatg aaactccatc gaaatacctg cggccgttaa cgtcgatcag gtaatgccct
     7021 tcgccatggc tgataatgca cggatcctcg cccatccatt cctgcatttg cgtgaacgga
     7081 tgccagagat agctatgatc tagctgcttg agcttatcgt aatctcgagt catgtggcgt
     7141 gattattaat cgaccgatgc gaatacctcg agcgcccgat ctatttgatc tgatgtgtgt
     7201 gtcgccataa gcgtgatccg caaccgcgac gttcctggcg gcaccgtcgg tggacggatg
     7261 ccctgagcga aaacaccgcg atcgaggagc cgactcgaaa ggcgcatgca ttcgttggca
     7321 tcgccgatca gcagcggtaa gatctggctt cggctgtcac ctggcgagta tcctaacttc
     7381 gtcaatccag accgtaatcg cgcgcaatta tcgcgcaaca cgcgccgcct ctccggctca
     7441 tgcttgatca attctagagc ggcaatgccc atcgccatga cggcaggcgg cagcgaggtt
     7501 gtaaagataa aacttcgaca ccgattgatc aggagctcac gcagcatggc gctacccgcg
     7561 atatacgcgc caaaccctcc cagcgccttt ccgagtgttc ccatctgtac gagcacacgc
     7621 tcggctagcc ctaactgtga aaccaggccc gcgccgtccg gctcgtagac tcccgtcgca
     7681 tgcgcctcat ccaccatgat catggcatcg tatttctcag acagctcgac aatctgcttc
     7741 agcggggctt cgtccccgtc catgctgaaa agcgattcag tgacaatcaa cttacgcgag
     7801 ctgcgcggcg cacgcttaag cccatattcg agttgctcga catcacaatg ccgatatacg
     7861 acgattttgg cccgcgacag gcggcaaccg tcgatgatgc tggcatgatt gagcgcatcg
     7921 ctgaagacca catcgccgtc gccgaccaga gttgggatga tccctgtgtt ggcctgaaag
     7981 ccggaattga aaactaatgc tgcctcggct cccttcaatt cggcaagctt ggcttcaagc
     8041 tcttcgtgca aagtcatatt gccggaaatc agacgcgaag cacccgaacc gcagccgtaa
     8101 cggccgatcg catcgatcgc cgcttttccc agcgccgggt gattggccaa tcccagatag
     8161 ttgttcgacg agaagtttaa tacttcgcgc ccatccacca tcaaagtcgg gccttggtcg
     8221 cccgcaacag accgaagacg gcgaaagagc gacgccgatt tgagagcctg cagttcgtct
     8281 tggatgaatc ccgccatcat ttggtgtgaa ctaataatac agaggtactg tcatcacaag
     8341 acgagatcac tatcgacggc tcggcagaga tgtatggaaa caaattcgag aaaattcacg
     8401 cagccgacaa gctctgtagc ttgcgcttaa ctaatttcat ctcagcagcg aaacgacggt
     8461 actcctgttc gcgctcgtca ttatcgcgct gccggagaat ggcagatgga tgaatagtga
     8521 caaaagtgag tttaccaaga acagagtcga tgaatctgcc gcgctctttc ataatcgcta
     8581 gaggcttacc caccacagac tgtgcggccg tagcccccat gcagacgaga atctgcggct
     8641 gcactgcttc tatctcgacc tcgagccacg gcctgcatgc aaccacctgg cgaacagaag
     8701 gcttctgatg taggcgccgc ttgccacgcc agatccactt gaagtgcttc accgcattag
     8761 tcacgtatac ctcatcgcgg gaaatgcgcg cgtcttccag cgcccggtca agcatgcgac
     8821 cagccggccc gacgaagggt gcaccttttt gatcttcgat gtcgccaggc tgctcaccga
     8881 ccagcatcac actcgcatgg ctgggcccat cgccaaaaac cgtctgggta gcattgaggt
     8941 agagatcaca tcccttgcac gatcttgccg cctgccggag agattcaagc gaccggcttc
     9001 ggggcaggaa atcgctagca gaaatcttct cagctctttc gatcaccaca ttgggagcct
     9061 atcagttgca agttttgagt ctagatagcc ggctcgcaca attcgtgagg catgatgtag
     9121 tgcggagatt gcgccaattt gtcctggcaa caccgcctgg tttgctactt tcgctcagac
     9181 tggattaaac tctgtcaatt aatctggata gaaagataaa tggccaaacc caaaacattt
     9241 tatgcctgtc agagctgcgg ctatcaagcg cccaagtggc tgggcaaatg ccccgattgc
     9301 aatgaatgga acacgttcgc tgaggagagg cttgaaagag caaccagcgt acgcggtgag
     9361 ctcagcctgg gtacgaaaga agatccggcg ccgattcatg aaatcaatac ggcagaagaa
     9421 ggtcggctgc aatgtggcat tggcgagttc gatcgcgtat tgggcggcgg tttggtgccg
     9481 ggctcggtga ttctcatcgg cggcgatccg ggcattggca aatcgaccct cctactccag
     9541 gcatttgcgg cgataagcca gaaaggactg acttgccttt acgtctcggg cgaagaatcg
     9601 ccgcgtcaaa tcaaaatgcg cgccgagcga ttgggcatcg tcgcgcccaa tctactcgtt
     9661 cttagcgaaa cctctttgga gagaatcata gagcatgtca agaacatcca acccgggctg
     9721 ctcgtcatcg actcgatcca aactattttc agtccgacga ttccatccgc gcccggcagc
     9781 atagcccaag tgagagaaag ttctggctca atcatcgtgc tggcgaaaaa gaccggccta
     9841 actacattct tgatcggtca cgtcacgaag gacggcgcca tcgccggccc gcgcctgctc
     9901 gagcacatgg tcgataccgt gctttatttc gagggcgagc gcggccattc tttccgtgtc
     9961 ctgcgcgcgg tcaagaatcg gtttggctcg accaatgaaa tcggcgtatt tgaaatgaag
    10021 gaagcgggac tcaaagaggt gagcaatccc tccgaaattt ttctgatgga gcgcccgctt
    10081 caggtcccgg gttcggcggt ggtctgcagc atcgaaggca cacggcctat attgatagag
    10141 ctccaggcgc tcgtcagtcg ctcgttctta gctgtgcctc ggcggacaac tatcggtgta
    10201 gatcacaatc gggtcgcttt gctggtggcg gttttggaaa agaagatggg cgccaaactc
    10261 tttgatcagg atatctttgt caatgttgcc ggcggcgttc acgtggatga gccggcggtc
    10321 gacctcggga tcgtcgctgc tatcgcttct agcagccgtg agaacgttct cgatcctaaa
    10381 acggttttct tcggagaagt aggcctagcc ggagagatcc gcggcatctc tcaggccgaa
    10441 acccgcatca aggaagcagg aaaattaggc ttcgaacggt gcgttcttcc cgccagcaac
    10501 agcgcgcaac ttaaaaacat caaacacccc aaactgtccg gagtgagctc tctgaacgag
    10561 tgctggaagg tgatgttcta gagtttgctt gggcatgggg agcttcaatg attggtgatc
    10621 tactgtcgat cgaaattcgt ttccacacgg gtgagagtcg attgaa
//
LOCUS       DASUTG010000646         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_100798, whole genome shotgun sequence.
ACCESSION   DASUTG010000646 DASUTG010000000
VERSION     DASUTG010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100798"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF089_14890"
     CDS             <1..673
                     /locus_tag="VF089_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175294.1"
                     /translation="ARSRDRGENWQDISGPQSPMAYPEYNDPAGIKPYQMGQHEDGRV
                     YRDVHWVLRHPRSANRLYATTGIGTYRSDDDGKSWIKAEYGMGRAYAIPIAIHRDAPD
                     RIFLGAAENGPTSWLGHRTVRAGPYNTIKFSPDTSEKLGGAKTGILRSDDAGENWRRL
                     NNGLPDSYPHMTCGFALHPQDGNTVCVSYTDGSIYASNDAGDSWRKLDVDIPKLYGIR
                     LMAVG"
     gene            complement(978..1841)
                     /locus_tag="VF089_14895"
     CDS             complement(978..1841)
                     /locus_tag="VF089_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX6175295.1"
                     /translation="MNSRQLLKQLFKRDRLLVAPGCFDGLSARLVEEAGFEAAYLSGG
                     ALARSMGIPDIGLVTMSEVIERAAQVVAAVKIPVIADADTGYGNAVNLVRSVREFERT
                     GVAAIHIEDQITPKRCGHLDGKEVIPLVEMEKKLQAALASRSDPDFLIIARTDARGVH
                     GFDDAINRGRAFAKLGVDAIFVEAPQSEAELEEIPRALTDVPLLVNVFKGGKTPMLPV
                     ERLQQMGYRIAIYPSETQRAAIHAMRQALALLKRDGTTEKMDDALTTFKERDKVVGLD
                     EWQQLEKQYMA"
     gene            complement(2097..>3203)
                     /locus_tag="VF089_14900"
     CDS             complement(2097..>3203)
                     /locus_tag="VF089_14900"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016958119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX6175296.1"
                     /translation="HPSDNLGGILALADYLTRSGQRAFTVGDVLTAMIKAHEIQGVLA
                     LENSFNRVGLDHVLLVRVATAAVASAMLGGSREQIVSALSNAWTDGGSLRLYRHAPNT
                     GPRKSWAAGDATSRGVRLALMAMRGEIGYPSALTAKEWGFYDVLFHGNPFKLGRPFGS
                     YVMENILFKISYPAEFHAQTAVECALRLHPQVSHRLEAIEKVRITTNESAIRIIDKSG
                     PLYNPADRDHCLQYMTAIGLIFGDLTADHYQDALAKDPRIDVLRAKMEVVEDLRYSKE
                     YLDPDKRSIANAVQVFFTDGSASENIAVEYPVGHRRRRAEGMPLLVKKFETNLASRLA
                     PPWCEKILDLCLDLKALHSTPVNVFTDMFTVHSA"
BASE COUNT          688 a          937 c          905 g          673 t
ORIGIN      
        1 cgcccgcagt cgggatcggg gcgaaaattg gcaggatatc agtggaccac agagtccgat
       61 ggcgtatccc gagtacaacg atcctgccgg tatcaagccc taccagatgg gacagcatga
      121 agacggtcga gtctaccgcg atgtacactg ggttctgcgc catccgcgca gcgctaatcg
      181 actctacgca accaccggga taggcaccta ccgctccgac gacgacggta aatcgtggat
      241 caaggcggag tacggtatgg ggcgggcgta cgccattcca attgcgatcc atcgcgacgc
      301 cccggacaga atctttcttg gcgccgctga aaatggaccc acatcatggc tcggccaccg
      361 caccgtgcgc gccgggcctt acaacacgat caaattcagt cctgacactt cggagaaact
      421 gggcggcgcg aaaaccggaa tcttgcgcag cgacgatgcc ggagaaaact ggcgccgcct
      481 gaacaacgga ttgcccgact cttacccgca catgacttgc ggcttcgctt tgcatccgca
      541 ggatggcaac accgtgtgtg tgagctatac cgacgggtcg atctatgcga gcaacgatgc
      601 cggcgacagc tggcgtaagc tcgacgtgga cataccaaag ctttatggta tccggctgat
      661 ggcggtcggt tgatttcaga agggcgcttt gccagaacct ggagcctgca gttcaaaatg
      721 atacagaatc gagcggcaaa cactccggag cgaagacaat aggcctctgc cgataacggc
      781 cggcaagctc cactaattta ttggacgcct gagaaattgc cagcccaggg caagcaccat
      841 agaaggcatt gagattgttc gcttctgtgt cttcgctcct ccaggctcgc ccctcgatcg
      901 ctatatgggt gtccatctct actgcgcaat ccgggataaa acagacgctc ggtgtgttcc
      961 gaatcaggat ctttcgctta cgccatgtac tgcttttcca gctgctgcca ctcgtccaga
     1021 ccgacgactt tgtcacgctc tttgaaggta gtcagcgcat catccatttt ctcggtggtg
     1081 ccatcgcgtt taagcagcgc gagcgcctgt ctcatggcat ggatcgcggc tctttgcgtc
     1141 tcggacggat agatggcgat gcggtaaccc atctgctgca ggcgttcgac cggtagcatc
     1201 ggcgttttgc cgcctttgaa gacattaacc agcaggggca cgtcagtcag ggcgcgagga
     1261 atctcctcaa gttcggcttc agattgcggc gcttcgacaa aaatggcgtc gacgccaagc
     1321 ttagcaaagg ctctgcccct gttgatggca tcatcgaagc catgcacgcc gcgcgcatcc
     1381 gtccgcgcaa tgatcaagaa atcgggatcg ctgcggctgg ccagcgccgc ttgcagtttt
     1441 ttttccatct cgaccagcgg aattacttcc ttgccgtcga gatggccgca tcgcttgggc
     1501 gtgatttggt cttcgatgtg gatcgcggca accccggttc gctcgaattc tcggacgctg
     1561 cgcacgaggt ttaccgcatt gccgtagccg gtgtctgcgt cggctatgac cggaatcttt
     1621 acggcagcga ctacttgcgc cgcacgctcg atcacctcgg acatagtcac caggccgata
     1681 tccggtatgc ccatgcttcg cgccagcgcg ccgccgctta gataagctgc ctcaaagccg
     1741 gcttcctcga ccaggcgcgc cgacaaacca tcgaaacatc caggggcaac caaaaggcga
     1801 tcacgcttaa acagctgctt gagtaattgt ctggaattca ttctgttctc cgatcttctt
     1861 gccgtagaat ttccgaaatg gcacagcaat tgagggcgag ccctctagag caaaggcaag
     1921 accttcctga ctgatagcgg cccgcgtgct tcagtggttt agcggtgtcg gatttatcag
     1981 ctcaggccca agcactccag acggcattga aataattcgc cccgtgcctt cgctcctccg
     2041 ggctcacccg atcggtgcac ggttgttcat ctccccctgc aaattgcaaa ttaaattcat
     2101 gcagagtgta cagtgaacat atcggtgaaa acattgaccg gagtcgagtg caacgccttc
     2161 aaatccaagc acaggtcgag aatcttttca caccacggag gcgccagccg ggaagccaga
     2221 ttggtctcaa acttttttac cagcagcggc atgccttcgg cgcggcgccg gcgatggccg
     2281 acggggtact cgaccgcgat gttttcagag gcagagccgt cagtgaaaaa gacctgcacc
     2341 gcattggcta tcgagcgctt gtcaggatcg agatactctt tcgaatagcg cagatcctcg
     2401 acgacttcca ttttggcacg caggacatcg atgcgcgggt ctttggcgag ggcatcctgg
     2461 taatgatcgg ccgtaagatc gccgaagatc agaccgatgg cggtcatata ttgcagacag
     2521 tggtcgcgat cggctgggtt gtagagcggt cccgacttgt cgatgatgcg tatcgccgat
     2581 tcgttcgtgg taatccgaac cttctcgata gcttcaagtc gatgactgac ttgcgggtgc
     2641 agccgcaagg cgcactccac cgcggtctgc gcgtgaaact ctgccggata ggaaatctta
     2701 aagaggatgt tctccatcac gtaagagccg aaggggcggc ccagcttgaa tggattgccg
     2761 tgaaacagca cgtcatagaa gccccactct ttagctgtca acgcagacgg atagccgatc
     2821 tcgccgcgca tagccatgag cgcgaggcgc actcctctgc tggtcgcatc gccggccgcc
     2881 caggatttgc gcggtccggt gttgggcgcg tgacggtata ggcgcaggct gccgccatca
     2941 gtccaggcat tggaaagcgc gctgacaatc tgctctcgac tgccgccgag cattgcgctt
     3001 gccaccgccg ccgttgccac tcggaccagc aatacgtggt cgagaccaac gcggttgaaa
     3061 ctattctcca gcgcgaggac cccctggatt tcgtgcgctt tgatcatcgc ggtcaggaca
     3121 tcgccgacgg tgaacgcgcg ttgccccgag cgagtcaagt aatcggctag agctaaaatg
     3181 ccgccaagat tatccgaggg gtg
//
LOCUS       DASUTG010000647         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_248631, whole genome shotgun sequence.
ACCESSION   DASUTG010000647 DASUTG010000000
VERSION     DASUTG010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248631"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1479
                     /locus_tag="VF089_14905"
     CDS             268..1479
                     /locus_tag="VF089_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6175297.1"
                     /translation="MTLPLERFRMLDLTRLVPGPMATWMLADLGMDVLKIEDVADRSA
                     YRDQQAARRSRENFTANQKMREEELRANAWDHTHRNKRSLAINLQKPEGQAIFHRLVK
                     DADVFFGSFRLGVYKRLGADYERLSKINPRLIYATISGYGFGNSFTEWPGNERNAQGM
                     SGVSALMTGQDGEPADMLCQPVDSYSAALAVIAIQSALFAREQTGRGQQVDISLVEGG
                     TAFVNSNVPDYFRNKKVRQRGFPSIGNLRCKDGKYLSSAAFAQAHFWAPFCDAIGLPE
                     MRDTVPAGNAEMIRKVKSHMLTKTRDEWLAIIPREISVVPMLEFDEVLEGPYAKERGM
                     VLELDHPLEGKVRQLASPFRLSDTPPSFRRFAPALGEHSVEVLQSIGYSENEIGALEK
                     AGVVRTQNFQP"
     gene            complement(1521..2558)
                     /locus_tag="VF089_14910"
     CDS             complement(1521..2558)
                     /locus_tag="VF089_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175298.1"
                     /translation="MPVRKHSNKLGGFKWRATNALSLIVAAFIFVATTGAARAADQVT
                     TAYQSVSIGSSTPLWIAKDAGFFERQGLDVKIVFVEGSPRTLQTLIAGEAQIVESTGP
                     AVLNARAAGSPVVIVAGYVNVMPYYLIVDKSINSPADLKGKVGANHMPGTAADTVIRI
                     GLKALGLDPDRDVSLRAIGNTPMRLQAMSAGAAQFMVAQDLELQQAKRLGYKVLVDYV
                     ANKTAFPMGGAVTTQPYVREKRDVVLRYVKAMAQALHYLKTNREGSLAIISRYARFQN
                     PEVMGAAFDSARRLYSEIPMPPTEGFDLVAKELAQRNPKLRDIDTSSVVDLQFVRELE
                     QSGFLKSLKGN"
     gene            3376..3519
                     /locus_tag="VF089_14915"
     CDS             3376..3519
                     /locus_tag="VF089_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175299.1"
                     /translation="MIIRERDGISENEIRVSSKNSEDQQKKGKLILAVLEQLADGRRV
                     MAP"
     gene            3778..4200
                     /locus_tag="VF089_14920"
     CDS             3778..4200
                     /locus_tag="VF089_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175300.1"
                     /translation="MNHLLARQSSIPVSSYYEPDILISHQYFKVFRQKSHFAPEKKLM
                     FAVLADAIECFQKYLGVNGRRRRNLFLQAEAWIYSGDSSWPYSFENICEVLNINPKYL
                     RLGLIQWRVAHESQENPGRCRRKTLRYQNTVKANRVCM"
     gene            4425..5465
                     /locus_tag="VF089_14925"
     CDS             4425..5465
                     /locus_tag="VF089_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175301.1"
                     /translation="MRILTLAAVQVFLLASLALAQPKPRTVAELAAYQGPDREEILKA
                     GAQKEGKVVWYTTLTAYKEIANIFESKYPGVTVEAYRSNSTDLLKRFLSEAQAQRYVA
                     DVIETTPPTLMAGREAKLLQPYYSPHLKDYPDESKEEADGGKVFWTTDRESIIGLGFN
                     EKLISAGDAPKSFDDLLKPEYKGKFAVSGDATGARMIGAMIKVKGEEFVKKLATQNIK
                     MHMISGGALQELLSAGEFPMSFSIFRNHVLVAQAKGAPTQWRALDLTVANAGGSALPA
                     HANRPHAALLFIDFMLSPDGQEILEEKFRYASSTKKYPFKRWYPEKGLTVAQFEKQFN
                     KWQKLLRQITRR"
     gene            5569..>5884
                     /locus_tag="VF089_14930"
     CDS             5569..>5884
                     /locus_tag="VF089_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175302.1"
                     /translation="MNRTIDEAAPKIAVSQWRLGIHRNLLLFLATSAVVAYLVLPPFF
                     FLLSTSMTAETGVDAGGFTLQHYQSILGSLDDFGVLVWNSLLFSTGSALSALAFGTLL
                     AWL"
BASE COUNT         1356 a         1570 c         1695 g         1263 t
ORIGIN      
        1 agctagaaaa aaccgtcttg acgcccgcaa tcctcgggtc gtttccgatc aaggaggtca
       61 actcgcataa gggttttacg cggtgtttcg ggtaggttct tttcgccacc tgatcaggct
      121 cgtgccgtac gtgaaccacg attcatcgtt cgcaggcata caccgcagcg ttgggcgcac
      181 tatccaacca atcgtgcgtt ggtagtttct cgtgcatcgg gacggtgtgt tgtggtaccc
      241 atgatgaaac aaaaaggagt caatgaaatg acgctgccgc tagaacgttt tcgcatgctc
      301 gatctgaccc gcttagtacc ggggccgatg gcgacttgga tgctggccga ccttggaatg
      361 gatgtgctga agattgaaga cgtcgctgat cggagtgcct accgcgatca gcaggcggcg
      421 cgtcgctcga gagaaaattt caccgctaat cagaaaatgc gcgaagaaga gctgcgtgcg
      481 aatgcttggg atcatacgca ccggaacaag cggagccttg ccatcaatct gcagaagccg
      541 gaaggccaag cgatctttca tcggctggtg aaagacgcgg atgttttttt cggctcgttt
      601 cgtctcggag tttacaagcg gctgggcgcg gactacgagc ggttgagcaa gatcaaccct
      661 cgcttgatct atgcgaccat tagcggctac ggcttcggca acagttttac ggaatggccc
      721 ggcaacgagc gcaatgcaca aggaatgtcc ggagtgagcg cgctcatgac cgggcaggac
      781 ggtgagccgg cggatatgct ctgccagccg gtggatagct attcggcggc gttggccgtg
      841 atcgcgattc aatcggcgct ttttgcccgc gagcaaaccg ggcgcggaca gcaggtcgac
      901 atttctcttg tggaaggcgg cacggccttc gtgaattcca atgtgcccga ctacttccgc
      961 aacaagaaag tgcgccagcg cggcttcccg agcatcggca acctgcgatg caaagacggc
     1021 aagtacttga gcagcgcggc attcgctcag gcgcatttct gggcgccgtt ctgcgacgcc
     1081 atcgggttgc cggaaatgcg cgacacggtt ccggcgggta atgcggaaat gatccggaag
     1141 gtgaaatcgc acatgctgac caaaacacgc gacgagtggc tcgcgatcat acccagagaa
     1201 atttccgtgg tgcctatgct cgagttcgac gaagtgctcg aagggccgta cgccaaggag
     1261 cgtggtatgg tgctggagct ggatcacccg ctggaaggaa aggtgcgaca actggcatcg
     1321 cccttccgct tgtctgacac gccgccgagc tttcgccgct ttgcgcccgc gctgggcgaa
     1381 cacagcgtcg aggtgctgca aagcattggc tatagcgaga acgaaatcgg cgcactcgaa
     1441 aaggccggcg ttgtccgcac gcagaatttt cagccgtagt tcaggtcctt atcgatcccc
     1501 ctaaaagccg cgtgagcgga ctaatttccc tttaggctct tcagaaaacc gctttgctcg
     1561 agttctctta caaactgcag gtccaccaca gagctcgtgt cgatatcgcg tagctttgga
     1621 ttgcgctggg caagttcctt ggcaacgaga tcaaatcctt cggtcggcgg catagggatc
     1681 tcgctgtaca gacggcgggc agaatcgaaa gcggctccca tcacttcggg attctggaaa
     1741 cgagcgtagc gggagatgat cgccagcgag ccttctctgt tggtcttcaa ataatgcagg
     1801 gcctgagcca tggctttcac gtagcgcagc acaacgtcac gcttctccct cacataaggc
     1861 tgggttgtca cggcgccgcc catcgggaag gcggttttgt tagccacgta atccacgaga
     1921 accttgtaac cgaggcgctt cgcctgctga agctcgagat cctgagcgac cataaactga
     1981 gcggcgcccg ccgacatcgc ctgcaaacgc atcggtgtgt tgccgatagc gcggagcgac
     2041 acatcccgat ccggatccag gccgagcgct ttgaggccga tgcgaataac ggtgtcggct
     2101 gcggttccgg gcatatgatt agcgcccact ttgcctttca ggtccgctgg agaattaatg
     2161 gatttgtcga cgatcagata gtacggcatc acattgacat aaccggcgac gatgaccacg
     2221 ggcgatccgg ccgcgcgagc gttgagcacc gccggaccgg tggattcgac gatctgcgct
     2281 tctcccgcga tgagcgtctg cagggtgcgc gggctgccct cgacaaacac aattttcaca
     2341 tcgagtcctt gccgctcaaa aaaccccgcg tctttggcga tccacaatgg ggtggagctc
     2401 ccgatcgaca ccgattgata cgccgtcgtc acctgatcgg ccgcacgtgc cgcaccggtc
     2461 gtcgcgacga aaatgaacgc cgcaacgatt aagctgaggg cattcgtcgc ccgccacttg
     2521 aatccaccca gtttgttgct gtgttttctc actggcatag gtgccggatt actccaagac
     2581 gaaaaaaaag tctactactg ctcactgctc gagctcccgg gccgttctgg cgcaccagta
     2641 gctgaatgtc tgccgactgc catgcgtgct ctaacggcaa tgtatgtccc agttgaacga
     2701 agcggccggc gcgaactcgt cgatagatcc gcagcaaagc tgcgggttgc gtggagagca
     2761 atcgataata tgagagcgtc cgtaggcagt gtacgtaggc aaggaggata agagtctgct
     2821 gttagtattc tgctctatga agtagcgtga tcggtggcaa tgagtagtga atacaggctc
     2881 atgattcatt cagattgcaa aattaacggc cgttctggcg gctgcggtct ttggctagga
     2941 tgtgtgcaga gatcattagc aggggacatc ctccgcaatc gaaagacgtc gtgtaaaaag
     3001 catgcacacg aattagcggg gctgtgcaga ttttgctccg catttagaaa gagagctcgg
     3061 tccagagaaa gacgccgata agttttttca ctatgtcagc cccacttctg cgccaatgta
     3121 ccgcgcatca ggagatcgga cgccgatcac gttcgcttca cttttactgg agtatagata
     3181 ccgcgaatta cgcgctcggc tcgctggagc cgcggatcgc gttagagcaa agcccgatga
     3241 gcttagtaag ataataacgg aaatcgctat ccattggccg aaccgacatg aagcgccatc
     3301 ggttaattcc tgtcgttagc acagggcgag tgaaatcttt acggcgaagc tagtgatttg
     3361 gagcaagtgg tcgaaatgat tattcgcgag cgggatggca tttcagaaaa cgaaatccgc
     3421 gtgagcagca agaattctga agaccagcag aaaaaaggta agttgatatt ggctgttctg
     3481 gaacagctcg ccgatggaag gagggtgatg gcgccctaac accatcaaaa atcgcgcttt
     3541 accagccgct aacaatctct gcccaaaatc gctacttggc gatcgcggat tcgctcggat
     3601 tcggggtcaa ttgtgttgcc tctgatgttt gtgacccggt cgcccgcgcg atcgatcgga
     3661 gcaaatctct gatcctgatc actcaagtgc tgcgcaagga gttcaccact cagatattct
     3721 gacgcattcc ttgatggctc caatcttgct cgacaactca gccagggagc gagcgcaatg
     3781 aatcacctct tggcgcggca atcatcaatc ccggtgagtt cgtattacga accggacatc
     3841 ttaatttcgc accaatactt caaggtattc cggcagaagt cccatttcgc accggaaaaa
     3901 aagctcatgt tcgctgtctt ggccgatgcg atcgaatgct ttcagaagta tctcggcgtc
     3961 aatggccgcc ggcgccgcaa cttatttttg caggccgagg cctggatcta tagcggcgac
     4021 agctcatggc cctattcatt cgaaaatatc tgcgaggttt tgaacatcaa tccaaaatac
     4081 ttgcgcctgg gtttgatcca gtggcgggtt gctcatgagt cacaggaaaa cccgggcagg
     4141 tgccgtcgta aaacgctgcg ctaccaaaat acggtgaaag cgaatcgagt gtgcatgtaa
     4201 gcagaggaga attccagctg ctgtagcccg gagccacccg caagagcagg tatgtcggaa
     4261 gggtaaagaa gagcctttcc tttcatgtgc gcaacggaga atagccggaa catcgccgcg
     4321 tccggcatga gcacgaaccg aagcgcgcgc cggaaagaga tttgacgcga aaagctacgc
     4381 atattgccgg gcgagagcgc gatggcgaat cacaggaggt aactatgaga attttgactt
     4441 tggctgcagt gcaagttttc ctgttggcat cgttggcgct ggctcaaccg aagccgagga
     4501 cggtggcgga actggccgct tatcaaggcc cggatcgcga ggagatcctc aaggccggcg
     4561 cgcaaaagga aggcaaagtg gtctggtaca cgacgctcac cgcctacaag gaaatcgcca
     4621 acatcttcga atccaaatat cccggcgtga ccgtggaggc ttaccgttcg aattcgaccg
     4681 atttgctaaa gaggtttctg tccgaggcgc aggcgcagcg ctatgtcgcc gacgttattg
     4741 agaccacccc gccaacactg atggccggtc gcgaggcgaa actgcttcag ccttattatt
     4801 cgccgcatct gaaagattat cccgacgaat ccaaggaaga ggccgatggt ggaaaagttt
     4861 tctggaccac cgaccgggaa tcgatcatcg gtttggggtt caatgaaaaa ctgatcagcg
     4921 ctggagacgc gccgaagagc tttgacgatc tcttgaagcc cgaatacaag ggcaagtttg
     4981 ccgtcagcgg cgatgccacc ggcgcgcgca tgatcggcgc gatgatcaaa gtcaagggag
     5041 aagagtttgt caaaaaacta gcgacgcaga atattaaaat gcacatgatc tccggtggag
     5101 cgttgcagga gctcctgtcg gcgggggagt tcccaatgtc cttttccatt tttcgcaatc
     5161 acgtgctggt agctcaggca aagggcgcgc cgacgcagtg gagagcgctc gatctgacgg
     5221 tggccaacgc aggcggctcg gcactgccgg cacacgctaa tcgaccgcac gcggcgctgc
     5281 tttttatcga tttcatgctc agtcccgacg gccaagaaat cctcgaagaa aaatttcgtt
     5341 acgccagctc gacaaaaaag tatcctttta aacgttggta tccggaaaag gggctgacgg
     5401 tggcgcagtt cgaaaagcag tttaacaaat ggcaaaaact gctgcgtcag attacccggc
     5461 gctaacagca gttttatcta ggcggatctg aggtagagcc ctgacggcca gggcgccagg
     5521 cgccacttcg cgggatccgg taaaaatcgt ttcgttcccg gcaatgtgat gaatcggacg
     5581 atcgacgagg cagcgccgaa aatcgcggta tcgcagtggc ggctcggcat ccatcggaac
     5641 cttcttctat ttctggccac aagcgccgtc gttgcctacc tggttttacc gccgttcttt
     5701 tttttattat cgaccagcat gaccgccgag accggagtgg atgccggcgg ttttactctg
     5761 cagcattacc agagcatcct tggctcgctc gatgatttcg gcgtgctggt gtggaactcg
     5821 ctgctgttct ccaccggaag tgctctcagt gccctggcgt tcggtacttt gctcgcatgg
     5881 ctgg
//
LOCUS       DASUTG010000648         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_990654, whole genome shotgun sequence.
ACCESSION   DASUTG010000648 DASUTG010000000
VERSION     DASUTG010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990654"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="clpX"
                     /locus_tag="VF089_14935"
     CDS             complement(<1..795)
                     /gene="clpX"
                     /locus_tag="VF089_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6175303.1"
                     /translation="MPKHGDDRSGNLVCSFCGKSQDEVRKLIAGPTVYICDECIDLCN
                     DIIAEECDHDETLTASSNVPKPAEIKRVLDQYVIGQERAKRILAVAVHNHYKRIDSQM
                     VTGDVELQKSNILLLGPTGSGKTLLAQTLARFLQVPFTIADATSLTEAGYVGEDVENI
                     ILNLLQAADYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTM
                     ASVPPKGGRKHPQQEFLQVDTTNILFICGGAFVGLEDIIRKRTGKKR"
     gene            complement(817..1419)
                     /gene="clpP"
                     /locus_tag="VF089_14940"
     CDS             complement(817..1419)
                     /gene="clpP"
                     /locus_tag="VF089_14940"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX6175304.1"
                     /translation="MNFIPMVVEQTGRGERAYDIYSRLLKDRIVFLGSVVTDDVANLI
                     TAQFLFLESEDPERDIFFYINSPGGSVTAGLAIYDTMQYVRPQISTVCVGQAASMGAV
                     LLAAGSKSKRYALPHARIMIHQPLGGFQGQAADIDIQAREILRMREELNNILMKHTGQ
                     SLKKIEKDTDRDMFMSGKQAQEYGLVDEVIAARPEGILKK"
     gene            complement(1450..2769)
                     /gene="tig"
                     /locus_tag="VF089_14945"
     CDS             complement(1450..2769)
                     /gene="tig"
                     /locus_tag="VF089_14945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6175305.1"
                     /translation="MKVDFAELSPVQRKVYIELPADEVASEFSRAYQNLGKRVRIKGF
                     RAGKAPRSVLQGIYGDEIREQVRSQLVEHSLGEVIKDRGLQIVSRPEIEAAELQENGQ
                     FSFSAVFEVKPEIDVNNYLGIEVEKPRIAVADDQVDEALKRLQQSHARLEPVTDRTVA
                     ERGDFIAVDFAGSVDGKPFSGGKGENYIIELGAGQALPQFDDALRGAKQGEEKQIKVT
                     YPEDYPNRGLAGKTVEFSVVVREIKQKVLPAIDDDFAKDHGECSSLEELKEVIRQRLQ
                     AELAQYQQEELKEQILNHLIESHSFEPPASMVERQTRYLLERNGTSAANSPEGKTAPT
                     TEETRKALEARAVRQVQATLLVEKISQLEKIDVEDRDIQERIDKLVRAAGERGKTLRE
                     FYSRPEARADLRTQIAFERTLDFLLQRANVKEVDASLNKVDDENKKS"
     gene            complement(2860..2944)
                     /locus_tag="VF089_14950"
     tRNA            complement(2860..2944)
                     /locus_tag="VF089_14950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2908..2910),aa:Leu,seq:tag)
     gene            complement(3532..4053)
                     /locus_tag="VF089_14955"
     CDS             complement(3532..4053)
                     /locus_tag="VF089_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175306.1"
                     /translation="MEPTAMTYSMTLEVYLQSAVLRGVLVTNQDRLSNYLILREGDEV
                     LSITNATLDDLQGKTMAAGVAEYLIYMQEVFLIGDLSTQLSSDRSGYEHLYVKKDSNK
                     ALISVGPYWLKGDIHVVPGAPLHELLLGKTRFFPITEATLIGRPDIAPRTYLVNRTKI
                     GCMTAVGDGLVEL"
     gene            complement(4338..4413)
                     /locus_tag="VF089_14960"
     tRNA            complement(4338..4413)
                     /locus_tag="VF089_14960"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4378..4380),aa:Lys,seq:ctt)
     gene            complement(4477..4552)
                     /locus_tag="VF089_14965"
     tRNA            complement(4477..4552)
                     /locus_tag="VF089_14965"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4517..4519),aa:His,seq:gtg)
     gene            complement(4566..4642)
                     /locus_tag="VF089_14970"
     tRNA            complement(4566..4642)
                     /locus_tag="VF089_14970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4606..4608),aa:Arg,seq:tct)
BASE COUNT         1131 a         1274 c         1101 g         1205 t
ORIGIN      
        1 gcgttttttc ccagttcgct tacggatgat atcttcaaga ccaacgaaag cgccgccaca
       61 gataaataga atgttagtcg tgtctacctg taaaaactcc tgctgcggat gctttctgcc
      121 gccttttggt ggaacgctcg ccatggttcc ttcgatgatc ttcagcagag cctgttgcac
      181 accctccccc gagacatcgc gggtaatcga cggattatcc gattttcgag aaattttatc
      241 gatctcgtcg atatatacga ttcccctctg agccttttca acgtcataat ccgccgcttg
      301 cagaaggttc agaattatat tttctacatc ttcgccaacg tatcccgcct ccgtgagact
      361 ggttgcatca gctattgtaa agggcacctg cagaaagcgt gcgagagtct gtgctaaaag
      421 tgtctttcca gaacccgtcg ggccaagtag caaaatgtta ctcttttgca attcaacatc
      481 gcccgtcacc atctgactgt caatgcgttt atagtggtta tgaaccgcta cagcgaggat
      541 tctttttgct cgctcctggc cgatcacata ctggtctaga actcttttga tttcagctgg
      601 ctttggaaca ttggatgaag ccgtaagagt ttcatcatga tcgcactcct cggcaatgat
      661 gtcgttgcaa agatcgatgc attcgtcgca gatgtatacg gtcggtccag caatcaactt
      721 gcgaacctca tcttgactct tcccgcagaa agagcaaacg agattcccgg atcgatcatc
      781 cccgtgcttt ggcatagaac ccccgcttca tttcaatcac tttttcaaaa tgccttcagg
      841 tcgagccgcg atcacctcgt caacgagacc atattcctgc gcttgtttac ctgacatgaa
      901 catgtcgcga tcggtgtcct tctcaatttt cttcaagctc tgcccggtgt gtttcattag
      961 aatgttattc aactcttcac gcattctaag aatctcacgc gcttgaatat caatatcggc
     1021 cgcctgcccc tgaaatccgc cgagcggctg gtgaatcata atccgggcat gaggcagcgc
     1081 gtaacgtttg cttttgctcc cggcggcaag cagcacggct cccatgctgg cagcctgccc
     1141 cacacaaact gtggagatct ggggtcggac gtattgcatc gtatcataga tcgctaaacc
     1201 tgcagtcaca gatccgcccg gagaattgat ataaaagaag atatcgcgtt caggatcttc
     1261 cgattcgaga aagagaaact gagcggtaat tagattcgcc acatcatcgg tcacaacgga
     1321 ccccaaaaac actatgcgat ctttaagcaa tcgagaatag atgtcatagg ctctctcgcc
     1381 gcgcccggtt tgctcgacga ccattggaat gaaattcatc ttagcaaact ccttaactac
     1441 gagggcattc taactttttt tattttcgtc atcaacctta tttaaagacg catcaacctc
     1501 tttgacattc gctcgttgca acaagaaatc gagagtgcgc tcgaaggcta tctgggttcg
     1561 cagatcggcg cgcgcttcgg gcctggaata aaactcgcgc agggtcttcc cacgttcgcc
     1621 agcagctcta acgagcttat cgattctctc ttgaatgtca cgatcttcga catctatttt
     1681 ttctagctgt gaaatcttct caaccaacaa tgtcgcttgt acttggcgga ctgcacgagc
     1741 ctcaagcgct tttctggttt cttcggtggt cggagctgtt ttaccttccg gcgagttggc
     1801 agccgaagtt ccgtttcgtt ccaacagata tcgagtttga cgctcgacca tcgacgcggg
     1861 cggctcgaac gaatggctct caatcaagtg gtttagtatc tgttccttga gctcttcttg
     1921 ttggtattgc gcgagctcgg cttgcaatcg ttggcggata acctctttca actcttctaa
     1981 cgaggagcat tcaccatgat cttttgcgaa atcgtcatcg atcgccggaa gaaccttctg
     2041 cttgatctca cgcacgacga ctgaaaactc gacagttttg ccggcaagac cacgattggg
     2101 gtagtcttca ggataagtga ccttgatttg tttctcctca ccctgcttgg ctcctcgcaa
     2161 agcatcatca aattggggca atgcctggcc ggctcctaat tcaataatat agttctcgcc
     2221 cttgccgccg gagaaaggct tgccatccac agagccggca aagtccactg cgataaaatc
     2281 cccccgctcg gcaacagtcc gatcagtaac cggctccaac cgcgcatggc tctgctgtaa
     2341 acgctttaat gcttcatcca cctgatcgtc tgccacagca atacgaggct tttcaacctc
     2401 gatgccgaga tagttgttaa catcgatttc aggcttaact tcaaaaaccg ctgaaaagga
     2461 gaactgtccg ttttcttgga gctccgcggc ctcgatctct gggcgagaaa ctatttgcaa
     2521 accccgatct ttgattactt ctccgagtga atgttccacg agttgtgaac gcacctgctc
     2581 tcttatttca tctccataaa ttccctggag cacacttcgt ggagccttcc ccgcgcgaaa
     2641 tcccttaatg cgaacccgtt tcccgaggtt ttgataggcg cgagagaatt cactggcaac
     2701 ttcgtctgcc ggtagctcga tgtaaacctt ccgttgtact ggacttagct cagcaaaatc
     2761 tactttcatg actctaaagc ggtgatccgt agctgtctac gcggttggca tggctgcgat
     2821 caccacacaa catctccttc ctgttgtgac gcacaaatct ggtgcgagag gggggactcg
     2881 aacccccatg gtgttacccg ctagatccta agtctagtgc gtctgccagt tccgccactc
     2941 tcgcgtcgga atgtccacca gcggtttgct cctggaccag caaaacagag taggaaaggc
     3001 gttccactaa gtcaagcttc gcaatgccta tgtcgaccga agaatgacat aatgccgtcg
     3061 tactgctgct gggtaacgat tgcgcgctaa atcaacggga gcggggcgaa gcactcttcg
     3121 cggcaccaga tgctatttta gagaggagta gatctcgggg tctggagaaa ctgaaggtaa
     3181 tcattaactg ccatgggttg atgaagtatc gcattctgga cagggacaga gagagcgtag
     3241 atagtcatat gtatagatgc cagtgctatg gccatcgtgc cattgaaatt ctattgcgta
     3301 gtttcctatc gggcgaatgt caatcaactt agcatccggc actgagagaa actttcggct
     3361 ggcgccgtgc ccctggcaga gagcacacgg gcaatggccc cggaggtatg tttctggata
     3421 aattcccgca tggccatcat cccacagaat cctgacgacg cgctctgctt tgctgtgatt
     3481 gatttccttt gggaattggc tcgtcatgcg ggctaaactt gcctttcagc atcaaagctc
     3541 aaccagacca tcccctacag ctgtcatgca accaatcttt gtgcggttaa ctaaatacgt
     3601 ccgcggtgct atatcgggcc tgccgatcaa agtggcctcg gtaatcggga agaaacgtgt
     3661 cttgcccaag aggagctcat gcagcggagc gccgggaacc acatgaatgt cgcctttcaa
     3721 ccagtagggc ccgacgctga tcaaggcttt gtttgagtcc tttttcacgt agaggtgttc
     3781 gtacccggac cgatccgagc tgagttgcgt gctcaagtcg ccgattagaa atacttcctg
     3841 catatagatc aaatattcgg caacgcccgc cgccatcgtt ttgccctgca aatcgtcaag
     3901 agttgcattt gtgattgaga gaacttcatc tccctcccga agtatcaggt aattacttag
     3961 acgatcctga tttgtaacca gcacgccgcg cagaaccgca gactgcaaat agacctccaa
     4021 agtcatggaa taggtcattg ccgttggttc cattagatct tttcctgttt acaagctgcg
     4081 tcttttgcac acgcgaatag aatcatgatc cattatctca ctacggagaa gccttaacaa
     4141 ttgatttctg gcgcggcatt acgttagcca gtaacgcagc gccgcgacgg gagaagccat
     4201 aaggaataca gctgtagggt cgagatatcc tgaagcacgc gggtctacat atacaccgca
     4261 cggctcaccc ggttggccaa ccgagtctct ctcatcactc gcgggataac gagttagcgc
     4321 cggagctaag aagagtttgg tgggccgccg gagactcgaa ctccggaccc gctgattaag
     4381 agtcagctgc tctaccaact gagctagcgg cccaaacaag gctttcacat cgaaacagct
     4441 cggcagctac gattcttcta ccgagcaata aaaaggtggg gtgagtgaag ggatttgaac
     4501 cctcaacccc tggagccaca gtccagtgct ctaaccgttg agctacactc accatatacc
     4561 cattctggcg cgccagaagg gattcgaacc cctgacctac ggcttagaag gccgttgctc
     4621 tatccaactg agctactggc gccaaaaaca acagcgagta ataagcgatg aggtaactaa
     4681 aactcaacac tcattactca gcagtcttga t
//
LOCUS       DASUTG010000649         7703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_396715, whole genome shotgun sequence.
ACCESSION   DASUTG010000649 DASUTG010000000
VERSION     DASUTG010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396715"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..391)
                     /locus_tag="VF089_14975"
     CDS             complement(29..391)
                     /locus_tag="VF089_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6175307.1"
                     /translation="MVEGEINKTILRVRRGRPGDAPRFAEFEVPFEDGMSVLDALRWI
                     RTHRDSTLAIRYSCINANACKTCMALVDGEVQYTCIAKLLPGIVTVEPLPKRRLIRDL
                     VTDILPEDEKAGFTEQAG"
     gene            complement(384..2090)
                     /locus_tag="VF089_14980"
     CDS             complement(384..2090)
                     /locus_tag="VF089_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX6175308.1"
                     /translation="MQEVSTDVLIVGAGGAGMYAAISAARQGASVLLLDKSLIGRGGA
                     TIMAQMTVAAALGQEEPDDCATHLRDTLEAGRGLCNEELSALLCYDGPQRILEMQEWG
                     TRWARAGERIKQEIAPGHSRKRCCYVDFLNTGPAVAATLRRKVSEVAAIRRLSNIAVV
                     DLVVENGRAVGAVGLDLAGGEFVVCHAKAIILAAGGLTKLYDRNSASSNMGGEAYALA
                     LWAGAELIDMEFVQFFPIGHLAPRLVGMDPIMWDPFRYKLGGKLLNGSFVEFVDRYGA
                     VDFGKYTATRDIAAYAILKEVEAGRGTPHGGAYLDFRHIPETQLRAAFGPVIDRLLTN
                     GIDLTKTPVEVGPTAHYHMGGVRVNGRMETCIQGLYAAGEAVGGANGANRLSGNAITE
                     AFVFGARAGACAGEQVKAISRDEDRKLTRVALDRIAALVPRGSKRGTAPVALQAELQK
                     LMWEKAGPFRTGEKLSDALRRIREMREQELPALEIATEGAFNMDLQDWFELRAMLTTA
                     EAVVTSALARTESRGAHQREDYPNLDPLYLRNQVLKLKNGALLSSWVNPVRLCAGLPT
                     NG"
     gene            complement(2130..2831)
                     /gene="rnc"
                     /locus_tag="VF089_14985"
     CDS             complement(2130..2831)
                     /gene="rnc"
                     /locus_tag="VF089_14985"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX6175309.1"
                     /translation="MKISSQLEALQKELGYSFKDPSLLVKALTHVSYDRKKTGSNNEV
                     LEFLGDAVLDLAISDLLIRRFPERSEGDLSRMRAGLVNSAVLAEKGSALALGPMLLLG
                     KGEERSAGRRKESILAGAFEALLGGVYQDGGYETARRIIERYFAADVAERTLGQQDYK
                     TRLQEISQLLFRVPPVYRLLSETGPDHAKCFVTEISVGGRVLGTGEGKSKKQSEQEAA
                     RQALNELQKSDVAGP"
     gene            complement(2841..4175)
                     /gene="mtaB"
                     /locus_tag="VF089_14990"
     CDS             complement(2841..4175)
                     /gene="mtaB"
                     /locus_tag="VF089_14990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX6175310.1"
                     /translation="MRIAVTTLGCKINQYDSAVIRARLEEKHSFVPFDEEADCYIINT
                     CTVTDRADWEARQLVRRAKRRSPSSKVLLTGCYAQVSPAEVARVQGVDFVVGLNRLDD
                     LLRLVETTDLNPPRIHVSDVKRQRGVPVLGTRALPGHTRAFLKIQEGCNYSCTYCIIP
                     TARGLSRSVTPHEVLNQVRLLADAGYAEIVLTGIHLGGYGQDLIPKMGLTALVELLVD
                     SSLVRRIRLSSLDPREVPERLLDLIAASKVICPHLHICAQAGEDTILKRMRRNYDTAY
                     YRELLSKTRERLPDAALGSDIIVGFPGETDEQFESSLAYFASLPLTYFHVFPYSERRG
                     TVASILPDRVPVPVKKVRAQRMRQLGAEKKEQFCQTFRGRRMPVLIEERIDRGSGNRC
                     GFTRNYLPVVVSGGNHRVNEEVDVQIDGYAGGCLTGAATYRRERGNRSEAGA"
     gene            complement(4263..5327)
                     /gene="mnmA"
                     /locus_tag="VF089_14995"
     CDS             complement(4263..5327)
                     /gene="mnmA"
                     /locus_tag="VF089_14995"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX6175311.1"
                     /translation="MIKRKPKVVVAMSGGVDSSVTAGLLVEQGYDVQGVSLRMWEGRL
                     GPRVCSDHRGASEIAEFLGIPHELLDLRSQFVETVVRPFAQAYTRGRTPNPCVACNRD
                     FKLGSLLQWAKQQHADYVATGHYARIERDNASGQSSLLRGIDEAKDQSYFLFALSQDQ
                     LAHTMFPLGKMRKSEVREKARLLGLSVADRPESQDICFGDYKALVRSHADAEDLGAGD
                     IVDRSGKVLGRHNGIHGVTIGQRRGLGIAAPQASYVLEIEQVSKRVVVGSKSELVCSG
                     LMAQRMNWLEPPVAQEFEAQVQIRYRSPAIPCVIRLAEKNGCRVRFAKTYPGVTPGQA
                     AVFFSGEKLLGGGWIEHAIR"
     gene            complement(5466..6197)
                     /gene="cysE"
                     /locus_tag="VF089_15000"
     CDS             complement(5466..6197)
                     /gene="cysE"
                     /locus_tag="VF089_15000"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX6175312.1"
                     /translation="MFDVVKEDIQVVFDRDPAARNVWEIIFTYPGFHALFFYRLAHAT
                     WVNGFKGLARFASNLGRFFTGIEIHPGAEIGRRFFIDHGMGVVIGETTEIKDDVTIYQ
                     GVTLGGTSLKKEKRHPTVEDWVVIGAGATVLGPVTIGHNSRIGSGSVVVSSVPPHSTV
                     VGIPGRVVEGEAARRDAHAHIIDLDHARLPDPIAKAIGALADYIQRLEKRVDELSDRQ
                     GGLEEQRAEQSEVIDKVKELLKHSS"
     gene            complement(6247..>7703)
                     /gene="cimA"
                     /locus_tag="VF089_15005"
     CDS             complement(6247..>7703)
                     /gene="cimA"
                     /locus_tag="VF089_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX6175313.1"
                     /translation="EGGYPGSNSRDADFFKQVKKLNLKNAKIASFGTTRKPGAKAAQD
                     LSLKILLSADAPVVTLVGKTWDLHVRDDLRISKKANLEVIADSIAYVKRRVDQVIFDA
                     EHFFDGFRSNPEYALECLQAAQQGGADWIVLCDTNGGRLPSEIRAAIGKVNESVTTPL
                     GIHCHNDGELAVANTMAAVEMGARQVQGTINGFGERCGNLNLISLIPNLQLKMGKNCI
                     KPNQLRKLREVSHFFYELANIQPNKRQPYVGDSAFAHKGGLHVSGVLKNRETYEHIDP
                     DFVGNRQRVLVSDLSGRSNVVYKGKEYGIDLEGQDNAVQEILRRTKELESQGYEFEAA
                     EASFELLIQQALGRKKKNFKLVGFRVIDEKRQEGEAPRSEATVMVEVDGVMEHAAAMG
                     NGPVNALDQALRRALTKFYPGLKDVELLDYKVRVLSSGEGTAASVRVLIESGDGKARW
                     GTVGVSHNVIEASWQALVDSIDYKLYKDARRRAN"
BASE COUNT         1592 a         2370 c         2047 g         1694 t
ORIGIN      
        1 gcggagttcg ccttatcata cagttggcct atcccgcttg ttccgtgaag ccggcctttt
       61 cgtcctccgg caaaatatcc gtgaccagat cgcggatcag ccgacgcttt ggcagtggct
      121 cgacggtaac aatgcccggc agcagtttgg ctatgcaggt atattgtact tcaccgtcca
      181 ccagcgccat gcaggtttta caggcattgg cgttgatgca gctgtaacga atcgccagtg
      241 tgctgtcccg atgagtccgg atccagcgca gcgcatcgag caccgacatg ccgtcctcga
      301 agggaacttc gaattcagca aagcggggag catcgccggg tcggccgcgg cgcacgcgca
      361 aaatcgtttt gttgatctcg ccttcaacca tttgttggta acccggcaca gagtcggacg
      421 ggattgaccc agctggatag caacgcgcca ttcttcagtt tcaggacttg gtttctaaga
      481 tacagcgggt caagattcgg gtaatcttct ctctgatgcg cgccgcggct ctcagttcgc
      541 gccagcgccg atgttaccac cgcttcagcc gtcgtcagca tcgcacgcag ttcgaaccaa
      601 tcctgcaggt ccatattgaa cgcgccctca gtcgcgatct cgagcgcggg cagctcttgc
      661 tcgcgcatct cgcggatccg ccgcaaagcg tccgagagct tttcgccggt acgaaacggt
      721 ccggccttct cccacatcag cttttgcagt tcagcctgaa gggcaaccgg cgcggttcct
      781 cgtttcgacc cgcgcggcac cagcgccgcg atccgatcga gcgcaacgcg agttagctta
      841 cgatcctcat cccttgagat ggccttaacc tgttcgccgg cacatgcgcc ggcgcgcgcg
      901 ccgaagacga acgcttccgt gatcgcgttg ccggacaaac gattcgcgcc gttggcgccg
      961 ccaactgctt caccggcggc atacaatccc tggatgcaag tttccatacg cccgttcacg
     1021 cggaccccgc ccatgtgata gtgcgccgtc ggacccactt caaccggggt cttggtgaga
     1081 tcgatgccgt tggtaagcaa gcgatcgatc actggtccga aggccgcgcg caactgcgtt
     1141 tccggaatgt ggcggaagtc gagataggct ccgccatgcg gcgttccgcg gccggcttca
     1201 acttccttga gaatggcgta ggccgcgata tcgcgagtgg ccgtgtactt tccaaaatcc
     1261 accgcgccat agcgatcgac gaattcaacg aagctgccgt tcagaagttt tccgccaagc
     1321 ttataacgaa aaggatccca catgatcgga tccatgccga ccagtcgcgg cgcaagatga
     1381 ccgatgggga aaaactgcac gaattccata tcgatcagct cagcgccggc ccacaacgcc
     1441 agagcgtaag cttccccccc catgttcgac gatgcactat tgcggtcgta aagcttggtg
     1501 aggccgcccg cggcaagaat gattgccttc gcatggcaga cgacgaattc gcccccagcg
     1561 agatccaatc caacagcgcc gacggcgcgg ccgttttcaa cgacgagatc gaccacggcg
     1621 atgttgctga ggcgcctaat tgcggcaacc tcgctcacct tgcggcgcag ggtcgccgcc
     1681 acagcggggc cggtattgag aaaatccacg tagcagcagc gcttgcggct gtggccaggc
     1741 gcgatttcct gcttgatgcg ctcacccgcg cgcgcccaac gggtgcccca ctcctgcatc
     1801 tcgaggatac gttgcggccc gtcatagcat agcagcgcgg aaagctcttc gttacagagg
     1861 ccccgacccg cttcgagcgt gtccctgaga tgagttgcgc agtcatccgg ctcttcctgt
     1921 ccaagcgcag cggccactgt catctgcgcc atgatggtcg cgccgccgcg gccaatcaga
     1981 ctcttatcca ggagcagcac cgaagcgcct tggcgcgcgg ctgaaatcgc cgcgtacatc
     2041 cctgcgccgc ctgcgcctac aatcaagaca tcggtggaga cttcctgcat ctataaactt
     2101 cgtttgatca ttgacatatt cggcgacgat caaggcccag cgacgtcgct tttttgtagc
     2161 tcgtttaatg cctgtctagc ggcttcctgt tcggattgct tttttgattt gccttcgccg
     2221 gtgcctagca ccctgccacc aacggagatc tccgtaacga aacacttggc gtgatcgggg
     2281 ccagtttcag agagcaaacg atagaccggg ggcactcgaa agaggagctg gctgatttct
     2341 tggagtctgg tcttgtaatc ctgttggccc agcgttcttt cggcgacatc agcggcgaaa
     2401 taacgttcga tgatgcgccg agcggtttcg tatcccccat cctgataaac tccgccgagc
     2461 aaggcttcga acgcgcccgc aagaatcgac tcctttctcc tcccggcact acgctcttcg
     2521 cctttgccca aaaggagcat gggtccaagg gccagcgcgc tgcccttttc ggctaggaca
     2581 gcggagttga ccaaccctgc gcgcatgcgc gataggtcgc cctcgcttct ttcgggaaaa
     2641 cgccggatca acaaatcact gatggccaaa tccagcaccg catcgccgag aaactccagg
     2701 acctcgttgt tgcttccggt ttttttccga tcataagaca cgtgcgtcag agccttcacc
     2761 agcagagagg gatctttgaa gctataaccc agctcctttt gaagcgcttc caactgtgat
     2821 gaaatcttca aggtcgcctg ctatgcgccg gcctcgcttc ggtttccgcg ctcccgacga
     2881 taagtggcag cgccggtcaa gcagccccca gcgtatccat cgatctgcac atccacctct
     2941 tcattcaccc ggtgatttcc accgctgacg accactggca aataattcct ggtaaacccg
     3001 cagcgattac cgctgccgcg atcgatcctc tcctcgatca gaaccggcat gcgccggccg
     3061 cggaaggttt ggcagaattg ttcttttttc tcggcgccga gttgtcgcat tctttgcgcg
     3121 cgcacctttt tgactgggac aggtacccgg tccggcaaga tcgacgctac agtgccgcgg
     3181 cgctcggaat acggaaagac gtgaaaataa gtcagcggca gcgatgcgaa gtaagccaat
     3241 gagctttcga actgctcatc tgtctcaccc gggaagccga cgatgatatc actgcctaac
     3301 gccgcgtcgg gcaatcgttc gcgggtttta ctcagcagct cgcggtaata ggcggtatcg
     3361 tagttgcgcc tcatccgttt gagaatcgtg tcctcacccg cctgggcaca aatgtgcagg
     3421 tgcgggcaga taaccttcga ggcggcaatg agatccaaca accgttccgg cacctcgcgc
     3481 ggatcgagcg agctcaagcg aattcgtcga accagactgc tgtcgacgag caattccact
     3541 agcgcggtca aacccatttt tgggattaga tcctggccat atccgccgag gtgaatcccg
     3601 gtaagaacga tctccgcata acctgcatca gccagcaaac gaacttgatt caaaacttcg
     3661 tgcggcgtga cactgcgact cagaccccgg gcagttggga tgatgcagta ggtacagcta
     3721 tagttgcagc cttcttgaat ctttagaaat gcccgggtgt gtccgggcaa ggctcgcgtg
     3781 ccgaggaccg gcacgccgcg ctgccgtttt acatcgctga cgtgaatccg cggaggattc
     3841 aagtctgtcg tttcgaccag gcgcagcaaa tcatcgagcc ggttgagccc gacgacaaag
     3901 tccacccctt gaactcgcgc cacctctgcc ggacttacct gagcgtagca gccggtcagc
     3961 agcaccttgc tgctcgggct ccggcgttta gcgcgccgca ccaattgccg cgcctcccaa
     4021 tcggctcgat cggtcaccgt gcacgtattg atgatgtagc aatcggcttc ctcatcgaaa
     4081 ggcacaaagg aatgcttctc ttccaagcgg gcgcgaatca ccgcagaatc gtactggttg
     4141 attttacaac cgagtgttgt caccgctatg cgcatagttt agccaatcga cagctagcaa
     4201 acagatagga ccgaacgctg agacattcta cccattgccg atacttcttt gctgatgcct
     4261 ggttagcgga tcgcatgttc aatccagcct ccgccgagga gcttttctcc gctgaaaaag
     4321 accgcggcct gtcccggcgt gacccccggg taagtctttg caaagcgcac ccgacaaccg
     4381 ttcttttcgg ccaaacgtat gacgcacgga atcgccggtg aacgataacg gatttgcacc
     4441 tgcgcttcga actcttgggc aaccggcggc tcaagccaat tcatccgttg cgccatgaga
     4501 ccgctgcaaa caagttcact tttgctgccc accaccactc gcttcgatac ctgctcaatc
     4561 tcaagaacat aagacgcttg cggtgccgcg atcccaagcc cgcgccgctg gccgatggtt
     4621 acgccgtgaa tgccattgtg cctgcccagc actttgccgg atctgtcaac gatgtcgccg
     4681 gcgccgagat cttcggcatc cgcatgagac cggaccagcg ccttatagtc accgaagcag
     4741 atgtcctggc tctcgggtcg atcagccact gacaaaccaa gcaagcgggc cttttctcgg
     4801 acctcgctct tacgcatttt gccaagagga aacatcgtgt gcgccagttg atcctgcgaa
     4861 agagcaaaca agaaatacga ctggtctttc gcctcatcga tgccgcgcaa cagggagctt
     4921 tgcccggaag cgttgtcgcg ctcgatccgg gcgtagtggc cggtagccac gtaatctgcg
     4981 tgttgctgct tggcccattg cagcaggctg ccgagcttga aatcgcgatt acaggcgaca
     5041 cacggattgg gcgtccggcc gcgcgtatag gcctgggcga acggtctgac aactgtttcc
     5101 acgaactgcg accgcaaatc caaaagctcg tgaggaatgc ccaggaactc ggcgatctcg
     5161 ctggcgccgc gatggtcgga acaaacgcgc gggccgagcc ttccttccca catgcgcagc
     5221 gatacgcctt gcacgtcgta gccttgctcc acaagcaagc ccgctgtgac cgaactgtcg
     5281 acgccgccgc tcatggcaac gacaactttt ggctttctct taatcattgg actgtgagaa
     5341 tgatttcacg ttatggccgt gcatagatgg taaccaaaga ggcaacggca cccactgaaa
     5401 aggcgcatac ttctcgagga gaaaccgttg tcttctttga cgttggctat tcgatgctct
     5461 cttattcaac tcgaatgttt cagcagctct ttaactttat cgatgacttc actctgctcg
     5521 gcccgctgct cctcgaggcc gccttgccgg tcggataatt cgtcgacccg cttttccagc
     5581 ctctggatgt agtctgcgag cgcaccgata gctttggcga tgggatcagg caatcgcgcg
     5641 tgatccagat cgatgatatg agcatgagcg tcccggcggg cggcttcgcc ttccactacc
     5701 cggcccggaa ttccgacgac tgtcgagtgc ggcggcacac tggagaccac aaccgatcct
     5761 gaaccgattc tactgttatg accgatagtc accggcccga gcacagtagc gccggctcct
     5821 ataaccaccc aatcctccac cgtcgggtgg cgcttctctt ttttcaaact ggtgccgccg
     5881 agtgtgactc cttggtaaat agtcacatca tccttgatct cggtggtctc gccgatcacc
     5941 acgcccatac catgatcgat gaaaaagcgc cggccgatct cggctcccgg atggatttca
     6001 attccggtaa agaagcggcc gagattgctg gcgaatcgag ctaagccttt gaacccgttg
     6061 acccaagtcg cgtgagcgag ccgatagaag aatagcgcgt ggaatccggg gtaggtaaaa
     6121 atgatctccc agacattacg ggctgccgga tcgcggtcga aaactacctg gatgtcttct
     6181 ttgaccacat caaacatgtg ttggctggct caaccctaat gtggtgacgg tcaaccacga
     6241 gcccgcttag ttggcccggc gcctggcgtc cttatagagt ttgtagtcga tgctgtcgac
     6301 cagagcctgc caactcgctt cgattacgtt gtgcgagacg ccaacagtac cccagcgcgc
     6361 cttgccgtcg cccgactcga tcaaaactcg caccgaagct gcggttccct ctccggagga
     6421 tagcacccgg actttataat ccaacaactc aacatctttg agaccgggat aaaactttgt
     6481 caatgctcgg cgcagagcct ggtccagggc gttgaccggc ccattgccca tcgccgccgc
     6541 gtgctccatc acgccgtcca cttctaccat aacggtcgct tcggagcgcg gcgcttcgcc
     6601 ttcctgtctt ttttcatcaa tgacgcgaaa gccgacgagt ttgaagtttt tcttttttct
     6661 acccagcgct tgctggatca gcagctcgaa tgaggcttcc gccgcctcga actcgtaccc
     6721 ttggctctcc agctccttgg tccggcgcaa aatttcttgc actgcattat cttggccttc
     6781 gagatcgatg ccgtactctt tgcccttata aacaacgttg ctgcggcccg acagatctga
     6841 gaccagcacc ctttgccgat tgccgacgaa atccggatcg atatgctcgt aggtctcgcg
     6901 attcttcaat acgcccgata catgtagccc gcctttgtgg gcaaacgcac tgtcgccaac
     6961 gtagggctgc cgcttattcg gttgaatgtt cgccagctca tagaagaaat gcgatacctc
     7021 acgcagcttc ctcagctggt tcggcttgat gcagttcttg cccatcttca attgcagatt
     7081 cggaatcagc gagatgagat tgagattgcc gcagcgttcg ccaaaaccgt tgatcgttcc
     7141 ttgaacctgg cgcgcgccca tttcaaccgc cgccatagtg tttgcaacag cgagctcgcc
     7201 gtcattgtgg cagtgaatcc cgagcggcgt tgtcaccgac tcattcactt tgccaatggc
     7261 tgcgcgaatc tcactgggca agcggccgcc gttggtatca cataggacga tccaatcggc
     7321 gccgccttgt tgagccgcct gcaagcactc caaggcatat tccggattcg agcgaaaacc
     7381 gtcgaagaaa tgttcggcat cgaaaatcac ctgatcgacg cgccgtttca cataggcgat
     7441 cgaatcggcg ataacttcca ggttagcttt cttactgatc cttaaatcat cgcgcacatg
     7501 cagatcccaa gttttgccga ccagggtgac gaccggtgca tcggcgctca ggaggatttt
     7561 gaggctcaaa tcctgcgctg ctttcgcgcc gggtttgcgg gtggtgccga atgaagcaat
     7621 tttggcgttc ttgagattga gcttctttac ctgtttgaaa aaatccgcat cccggctgtt
     7681 cgatccggga tagccgcctt cga
//
LOCUS       DASUTG010000650         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1284887, whole genome shotgun sequence.
ACCESSION   DASUTG010000650 DASUTG010000000
VERSION     DASUTG010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284887"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1221)
                     /locus_tag="VF089_15010"
     CDS             complement(274..1221)
                     /locus_tag="VF089_15010"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6175314.1"
                     /translation="MSRPLRVQYPGAVYHIMNRGRARQPTFVDDTDYRAFLDTLAEAH
                     RQWGIEMFAYCLMGNHYHLCLRTPKGNLSRIMRHVDGIYTQRFNRRHRRDGTLFRGRY
                     KGILIDADEYLAAVVRYIHLNAVAAGLVKMPEEYRWASHQYYLKAKGAPDWLDTTEAI
                     EQIGGRQVFHEFVLSGNEPSLRHYYEAKRQSSILGSETFLDRVRQRGEPASREHPRYE
                     RRVVRAGPERVLHEIMDQYKVKREEIFRGVRGRENEARKVALYVIKRCCDRTLPEIAE
                     YFGIGNYSTVSWSCRTVAAQMSREKNFRDRVEGIRTSIT"
     gene            complement(1437..2627)
                     /gene="ltrA"
                     /locus_tag="VF089_15015"
     CDS             complement(1437..2627)
                     /gene="ltrA"
                     /locus_tag="VF089_15015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX6175315.1"
                     /translation="MAYQEAKANDGAPGLDGMSFEDIEAQGVEKFLAAIGQELLTGTY
                     QPTKNRRVEIPKDNGKTRKLGIPTIKDRVVQGAVKLILEPIFEADFYISSFAYRPRRT
                     AHQALDRVVHGLVQGLTQVIDMDLKSYFDNIRHHHLLRKLAKRIEDPAILHLVKQILK
                     ANGKKGVPQGGVLSPLLANLYLNDLDKAMEEETVERRREGKWERVIYTRYADDMVVLV
                     DGYPQWRPHVEIVRKRLQEELKKVEVEINEEKTRVVDYASGGSFGFLGFDLREGRNRF
                     GKRFVLRTPMKKKQSELVRRVGRILKFSRHRKLSDVLEQIRPVIVGWVNYFRVGNARR
                     AFGWIRFEMMRKIRRFVMKQKGRRGCGWNRWSNETIYKEWGLVNNYTVQYFYRSPAKV
                     LPAR"
     gene            3379..4071
                     /locus_tag="VF089_15020"
     CDS             3379..4071
                     /locus_tag="VF089_15020"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HEX6175316.1"
                     /translation="MLKLKLNENDLNTANLYLRPDSVARISKLMPTAVDITARCQPER
                     GRVEAFIRAVYVRAYKADIAVSYPILMSVRDAGGTILAAVGFRYAKEEPLFLENYTGA
                     PIEQVLRTPRERIAEIGNLASAGGGASIFLFAALASYLNFKGIDYATVTGTDYLHRTF
                     KKLGLNPQKICDASLEAVQSDGQNWGSYYDTRPRVLAGSVPHSVSRLKKALGAEYQDC
                     RPRLFPRLHYRT"
     gene            4075..5472
                     /locus_tag="VF089_15025"
     CDS             4075..5472
                     /locus_tag="VF089_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6175317.1"
                     /translation="MSIILEAISRHKPDKIALIGDDQTLTYKELAAAIDDIALGLDSV
                     KVLGLALENGIEWVLWDLAAIRAGIVCVPLPPFFSAEQRDYVIKAAGISHIVTAEGIS
                     NTDAAYTNSIPSGTAKITFTSGTTGTPKGVCLSQAAMDRVALSLANTLGAEIAARYLS
                     VLPLSVLLENIAGVHATLLAGGTAHVCSPGHIGMGDPFKPDFVQLAHAISTDKITAAI
                     LVPELLRGLMQAVAGTKLSLPCLKFLAVGGSRVAPELVRQARALGLPVYEGYGLSECA
                     SVVALNTPGQDRPGTVGRVLSHIDLAVKDGEVVVRNPAFLGYLGSPHKGEFKTGDLGH
                     IDENGFLHIEGRCKNVLITSYGRNIAPEWIESHLLARPEIQQAVVYGDARPYLSALIV
                     SATETADITQAVETASKVLPEYAQVKDFHLVPPFTMQDGTLTGTGRPRRKAIFAKYQS
                     LIAKESNDELLRTAG"
     gene            5447..>5742
                     /locus_tag="VF089_15030"
     CDS             5447..>5742
                     /locus_tag="VF089_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6175318.1"
                     /translation="MSFYERLVKETEAQRQVLYNVSQLQDGLRGEIDVDSYIAYLTQA
                     YHHVQHTVPYLMAMGARLPESKKWLQGAIIEYLEEEVGHEEWILNDIEAAGGSK"
BASE COUNT         1224 a         1671 c         1442 g         1405 t
ORIGIN      
        1 tagacgtcaa cgatccgacc gcatcgtttc tgcagggtgt agccgagcct aattttcttc
       61 gctccgcatc agcgacggga agtgtcgtgg gcggcttcta aacagattcg gcatcaagcc
      121 aaacccgaat atctcacccg atgacacttc cttgattcac ttagtcttcg ttttcacgca
      181 gcagcgattt gtttcgttcc aacacttgca aggtgtgtgt tttccatctc gtccacagac
      241 atgccgcaat tgtggaaggg gtcaagtctg gagttacgta atgctcgttc tgattccttc
      301 aactcgatct cggaaattct tctctctact catctgcgct gctaccgtac gacagctcca
      361 actcacagta gaataattgc cgatcccgaa atactcggct atctcaggca acgtccgatc
      421 gcaacaccgc ttaatcacat acagcgctac tttgcgcgcc tcattctccc gaccccttac
      481 tccgcgaaag atttcttcgc gcttcacttt gtactgatcc atgatttcgt gaagcacacg
      541 ctccggcccc gcccggacaa cgcgtcgctc atagcgtgga tgctcccgac tggctggctc
      601 tcctcgttgt ctgacccggt cgagaaaagt ttcactgccg agtatcgaac tttgacgctt
      661 ggcttcataa tagtgcctaa gcgatggctc gtttcccgat agcacaaact catgaaatac
      721 ctgtcttccc ccaatctgtt cgatcgcctc cgtggtatcg agccaatccg gtgcgccttt
      781 tgccttaaga tagtactgat ggctcgccca gcggtattcc tcgggcatct tgaccagtcc
      841 cgccgctacg gcgtttagat gaatgtagcg taccaccgcc gccagatatt catcggcatc
      901 gatgagaatg cccttatagc ggccccgaaa caatgtccca tcccgcctat ggcgccggtt
      961 gaatcgctgg gtatagatgc cgtcgacatg gcgcatgatg cgcgagagat ttcctttcgg
     1021 agttcgcaga cacagatgat agtgatttcc cattaaacaa taggcaaaca tctcgattcc
     1081 ccattgccga tgggcttcgg ccagggtatc gagaaaagct cgatagtcag tgtcgtcgac
     1141 aaacgtcggc tgtcttgccc gacctcggtt cattatgtga tagacagcgc caggatattg
     1201 aactcggagc ggtcgcgaca tgtagtggat actacttcag aaggttataa ctagtgtagg
     1261 gtcgaggact ggcgcggtgt tccgtaggtg aagcctatct gttccgtctc tttcgtctga
     1321 cggtgcctca ctagccccac cgtaaatccg tttccagccc ccgccacgtc aaaccggact
     1381 tgaccctttc ggtcatccgg ctttcctgag cctgttcatc atgagacgct atttgcctag
     1441 cgtgctggaa gtactttcgc gggcgatcga taaaagtact ggactgtgta attgttcacg
     1501 agtccccatt ccttgtagat cgtctcgtta ctccatctat tccagccgca gccacgacgg
     1561 cccttctgct tcatcacaaa tcggcgtatc ttcctcatca tctcaaagcg tatccagcca
     1621 aacgctcggc gcgcgttgcc cactcggaag tagttgaccc aacccacaat cacaggccgg
     1681 atctgctcca gcacatcgct gagcttgcga tgccgggaga atttcaaaat tctcccaaca
     1741 cgcctaacca gctcactctg tttctttttc atcggcgtgc gcaaaacaaa tcgcttaccg
     1801 aatcgattgc ggccctctct gagatcaaat cccaaaaacc caaaactccc gccacttgcg
     1861 taatccacca cccgcgtctt ctcttcgttg atttctactt cgaccttctt cagctcttct
     1921 tgcagccgtt ttctcactat ctctacatgc ggccgccatt ggggataccc atcaaccaac
     1981 accaccatgt cgtcggcgta acgagtgtaa atcacccgct cccacttccc ttctctcctg
     2041 cgctcaaccg tctcctcttc catcgcctta tccaagtcat tcagatacag attagccagt
     2101 agcggcgaga gtactcctcc ttgcggaact cccttctttc cgttggcctt cagtatctgc
     2161 ttgaccaggt gcaggatcgc tggatcttct attctcttgg ccagcttccg aagtaaatgg
     2221 tgatgccgta tattatcgaa gtagctcttg agatccatgt cgatgacttg ggtgagccct
     2281 tgtaccagcc catggacaac tcgatccaat gcctggtgag ctgtacgccg gggccggtag
     2341 gcaaatgaac ttatgtaaaa gtcagcttca aagatcggtt ccaagataag ctttacagct
     2401 ccctgaacca cgcgatcctt gatggtggga attccgagct tgcgggtctt gccgttgtcc
     2461 ttgggaatct ctacccgtcg gttcttggta ggttggtagg tcccggtgag aagctcctgc
     2521 ccaattgcgg ctaggaactt ctccactcct tgtgcctcga tgtcttcaaa gctcattcca
     2581 tccagaccag gcgctccatc gttggccttg gcctcttgat acgccaatct tagggtctct
     2641 gcccttgtga catggacata cagaccccaa aaccgccacg tcttctcggc tttcgcctta
     2701 cggtatatcc ttcgcctcag ttcttgcaga tcaacggaag gttttatcat gccttccctg
     2761 cctcaccttg cgctcgaagg aatcaaactc agcatggccc cttcgctcca tccgaattac
     2821 cagacttcat cgctactacg gaccactccg ccatcctgtt tgccttcggt ccttttcctg
     2881 tgtccagtta tagggccgat ccttctccag gaatttctcc tggagcaagc aggacttctc
     2941 cagtttctgt cgtgtccctg ttaccgtgtc gccgccaata ccccgccggt gtgaatcatc
     3001 ccttcagcca gtctgaaatc attcatgctg tcttcgccga ttattgaacg gctcgaccac
     3061 cggatcttta atcttaacga ggcttgctca gcgttcacgc acgttgcaac ccggcaactc
     3121 gctcatcagc ccgaagctga ctttgtcagt aggcttcagc acgtcgatta ctctccgtgc
     3181 tgctactcaa gctaggcggc ttctggctct taccgccgcg ggacttccct catcactgag
     3241 agtcacctta tggatcacga cagcaattca tctggacaca ccaactccag atttgaccct
     3301 aagcccctcc aacaatttct ccttcaggtt cccttcaggt ttaaaagcaa cgcttgagta
     3361 taacttaagg agtctgcgtt gcttaagctg aagctaaacg agaacgatct taatacggca
     3421 aatctttacc tgcggcctga ttcggtggcg cggattagca agctgatgcc taccgccgtc
     3481 gatatcaccg cgcggtgtca gcctgagcgg ggacgggtgg aggcatttat acgggcagtt
     3541 tatgtccgag cctacaaggc cgatattgcc gtatcctacc ccatattgat gagcgttcgg
     3601 gatgccggcg gtacaattct ggcggcggtc gggtttcgtt acgccaaaga agagccgctg
     3661 tttcttgaaa actacaccgg cgcgccaatt gaacaagttc tgcgtacccc gcgggagcgg
     3721 attgccgaga tcggcaatct tgcttccgcc ggtggcgggg cgtcgatctt cctgttcgca
     3781 gcgctggcct cgtatctcaa tttcaagggc atcgactatg ccaccgtaac cggcacggat
     3841 tacctgcacc gaacgtttaa aaagcttggc ctcaacccgc aaaaaatctg cgacgcctcg
     3901 ctcgaagccg ttcaaagcga cggccagaac tggggaagtt attacgacac caggccgcgc
     3961 gtgttggccg ggtcggtccc gcacagcgtt tcgcggctga agaaagcgct gggggcggag
     4021 taccaggatt gccgcccgcg cctgttcccg cgcctgcatt acaggacata ggatatgagc
     4081 atcatacttg aagccataag caggcataag cctgataaaa tcgcgctgat cggtgatgac
     4141 cagacgctta cctataaaga gctggcggcg gcgattgacg atattgccct tggacttgat
     4201 tccgtgaagg tcttggggct ggcgcttgag aacggtatcg aatgggtttt gtgggatctc
     4261 gctgccataa gagcgggaat tgtttgcgta ccgctgccgc cctttttctc ggcggagcag
     4321 cgcgattacg ttattaaggc tgccgggatc agccatatcg taacagcgga aggcatcagc
     4381 aacaccgatg ccgcttatac aaattcaatt ccttccggca cggcaaaaat cacctttact
     4441 tccggtacga cgggcacacc caagggagtc tgcctgtcgc aggcggcaat ggaccgggtg
     4501 gcgctgagtc tggccaatac gctgggtgcc gagattgccg cgcgatattt gtcggtgctg
     4561 ccgctttcgg ttctgcttga aaatattgcg ggggttcatg cgaccttgct ggcaggaggc
     4621 actgcccatg tttgcagtcc cggtcatatc ggcatgggcg atccgtttaa gcccgatttc
     4681 gtccagctcg cccacgccat aagcacagat aaaattaccg cagcgatctt ggtgccggaa
     4741 ctgctgcgcg gcctgatgca ggcggttgcc ggaacgaagc tttctttgcc ctgccttaaa
     4801 ttcctcgcgg tcggcggttc acgggttgcg cctgagcttg taaggcaggc gcgggcgctg
     4861 ggcctgccgg tttatgaagg ctacgggctt tcggaatgtg cctcggtggt ggcgctgaat
     4921 acgccggggc aggacaggcc gggcacagtc ggcagggttt tgtcgcatat tgatcttgcc
     4981 gtgaaagacg gtgaagtcgt cgttcgcaat ccggcatttc tcggctatct cggcagtccg
     5041 cataaaggtg aatttaaaac cggcgatctt ggtcacatcg atgaaaatgg ctttttgcat
     5101 atcgaaggcc gctgcaaaaa tgttctgatt acttcttacg gtcgcaatat cgcgccggag
     5161 tggatcgaga gccatttgct cgcaaggccg gaaatccagc aggccgtcgt ttatggcgac
     5221 gcccggcctt atctgagcgc cctgatcgta tctgcaaccg aaacagccga tatcacgcaa
     5281 gccgtggaaa cggctagcaa ggtcctgccg gaatacgcgc aggtcaaaga ctttcatctg
     5341 gtgccaccct ttacgatgca ggacggcaca ctgaccggca cgggaagacc gcgccgcaag
     5401 gctattttcg ccaagtacca atcacttatt gccaaggaga gtaacgatga gcttctacga
     5461 acggctggtt aaagaaaccg aagcgcagcg acaggtgctc tacaacgtct cgcagcttca
     5521 ggacgggctg cgcggcgaga tcgatgtcga cagctatatt gcttacctga cgcaggccta
     5581 tcatcacgtc caacacacag tgccttacct gatggcgatg ggcgcacggc tgcccgaaag
     5641 caagaaatgg ctgcaaggag cgatcatcga atatctggaa gaagaagtcg ggcatgagga
     5701 gtggattctc aatgacattg aggccgcagg cggcagcaag ga
//
LOCUS       DASUTG010000651        13679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_544795, whole genome shotgun sequence.
ACCESSION   DASUTG010000651 DASUTG010000000
VERSION     DASUTG010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544795"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1215)
                     /locus_tag="VF089_15035"
     CDS             complement(91..1215)
                     /locus_tag="VF089_15035"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(835..837),aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HEX6175319.1"
                     /translation="MNARQRVALLTNGMTRQKTKGFLKMLRINGLAAGLMMLILAGVI
                     TAAAGSADLRPSDIVTVLPKDAIPAIMSPSFEEAGKASWLRHDNVVVGMSIAGESRAY
                     PVSILSRHEIVNDKLGGVPIAVTWUPLCYTAIVYDRRIKDHELTFGVSGKLHANSLIM
                     YDHQTDSLWSHLVGAAVTGPLKGEKLKPIQSMFTRWSTWKKLHPNSKVLSRDRGGFLT
                     SARDPYESYYRSADTGIVPTRQSDKRIYPKEYVLGLILNGKAKAYPYSVLSRRSVVND
                     EFAAVPLLIVFDEETATGVIFTRKLDGKTLSFKKAQLKSEKGLFLVDDESGSIWQGLT
                     GQAVQGSLKGSRLEALPITPSFWFGWIDHYPDTQLFELRK"
     gene            1418..1999
                     /locus_tag="VF089_15040"
     CDS             1418..1999
                     /locus_tag="VF089_15040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6175320.1"
                     /translation="MRIKSALAALIVLLSAAVWIYGRDSSPQPAAQTPATHLLADLGI
                     EPSQERTPAPAFLLNDSDGNVVRLDDLRGKVVLLNFWATWCPPCRLEMPSLEALHRQL
                     GPRGLAVLAVASRDNAGDVRSFFNEHRLSLPALLDPGAHAYGVYEIWSLPTSFVIDKR
                     GYLVGKVVGHRDWISEQSKDFFLHLLGGSTYIF"
     gene            2108..3307
                     /locus_tag="VF089_15045"
     CDS             2108..3307
                     /locus_tag="VF089_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6175321.1"
                     /translation="MDALSRINGANGKVAVTDFEAAVLQRYGDAAQEVEQCLCLPVSY
                     DKGLLGVIPQEIIDKDYGCGDPSRYVRQGETVLDLGSGSGKACYIIAQIVGPEGKVIG
                     VDFNPPMLDLARKYQESIGDRLGYRNIEFRRGKIQDLKTNLELVEGYLQAHPVVSVAD
                     LAKLEIYEDHIREKQPLIADDSIDVIVSNCVLNLVRPNDKRQLFTEMYRVLKRGGRVA
                     ISDIVCDEPVPEHLVNDPNLWSACVSGAFQEEEFMRAFEEAGFHGIRIEELRSEPYQT
                     VEGIEFRAVTLTAYKGKEGPCIERNHAVIYRGPWKQVVDDDGHRLERGARMAVCDKTF
                     KLYSQPPYAGEFILLPPREEVAAEAAGLFDCARDHRRHPRETKGEDFKVTRVSGSVCG
                     PGSDCCP"
     gene            3318..4277
                     /gene="arsS"
                     /locus_tag="VF089_15050"
     CDS             3318..4277
                     /gene="arsS"
                     /locus_tag="VF089_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX6175322.1"
                     /translation="MQAFVDKIRSYGGDFSRLAVDVLQVNMGRYCNLACLHCHVESGP
                     TRTEMMSRETVDAVLRFLASSNIPTLDITGGAPELHPNFDYLVTAVRQLKRHVMDRCN
                     LTVIFEPGKEYLPEFFKEHRVELICSLPCYSQENVDKQRGKGTFDASIRALQRLNQIG
                     YGNTDGDLVLNLVYNPVGPHLPPPQQKLEQDYKQILAENFGIAFNHLYCLTNMPITRY
                     GTHLKLRGEYDRYLDLLATNFNGATLDQVMCRNLISVGWEGLIYDCDFNQMVNLPIRD
                     RAGKPLHISSLSIEQLRNHAITVGDHCYACTAGAGSSCGGTLV"
     gene            4293..5411
                     /locus_tag="VF089_15055"
     CDS             4293..5411
                     /locus_tag="VF089_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX6175323.1"
                     /translation="MIDWSIIIAGATFFVGYANGANDNFKGVATLFGSGITEYRKALA
                     WATITTLAGSVAAFFLATELISIFQGKGLVPPELSNSEPFVAAVVLGTALTVMLATKL
                     GMPISTTHSLTGALCGSGLVAVGFGFGFKALAVNFFLPLLVSPFCAVAIANFAFPLLN
                     RLVQRAALNQENCLCVEAVAPSTVTPEGLVLFQTTPGLRVIMDREAVCAQPGSPTILG
                     INGQKLLDASHFLSAGAVSFARGLNDTPKIVALSLVAGAFDLRLSTLLVAAMIALGGL
                     FNAREVAQTVSKRITGMEPVHGLWANLVTSFLVIFASKWGMPVSTTHVSCGALFGIGI
                     ANGQARWKTIRTILMAWLVTLPLAALLSAGVFVVFRQL"
     gene            5505..7754
                     /locus_tag="VF089_15060"
     CDS             5505..7754
                     /locus_tag="VF089_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX6175324.1"
                     /translation="MKTSKTIVVILMLSAIIAGVLFLPVRQWFIHFEGYVQSLGAIGP
                     AAVVLAYILSTVLFIPGSALTIGSGTLFGLTTGFVVVFLGANLGALGSFLLARTLLRD
                     KVARWAEANPKFRSLDQAIGKQGFKMVLLTRLSPVFPFVLLNYFLGLTAVRTGSYVLA
                     NVIGMLPATFLFVYIGAAARDALAGQMDASAGFYQQILKVVGLLATVAVVVVVTRIAR
                     KALREAEQEQAGDGGREPHLNTNHQPASFETMVLIDDEHDRRLIENCHPPRWVNPAPA
                     AKYNLVVIGAGTAGLVSAAGAAGLGAKVALIERNLLGGDCLNVGCVPSKGMIRAARAA
                     HDAREGAKFGVRLTCEPEVGFAAAMERMRKLRAGISDHDSAERFRKLGVDVFIGSARF
                     TSPSSIDVDGIRLDFNRAVIASGARAAQLPVPGLDEVGYLTNETVFTLTELPRRIAII
                     GAGPIGCELAQAFARFGSKVSLLTDGADILPKEDRDAAAIVRKQMEHDGVRIVTGAKI
                     HRGTKNKQEKTLAVAIDGSTVELGCDAILVAVGRTPNLEGLDLEAAGISYTRRGVDVD
                     DRLCTTNPRVFAAGDICSRYKFTHAADAMARIVIANALFMARRKVSGLTIPWCTYTDP
                     EIAHAGYYEKDAQAAGFNVATLTESLDHVDRAILDGEEEGFARVHYDKKTGKILGGTI
                     VARHAGEMISELTLAMVAKQGVGVLSSTIHPYPTQAEAWRKIGDAYMRTKLTPRVKRV
                     FEKWLAWRR"
     gene            7808..8644
                     /locus_tag="VF089_15065"
     CDS             7808..8644
                     /locus_tag="VF089_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX6175325.1"
                     /translation="MLKGSVCRLRFFSGVALLAIFGAPQADLWPKWQKHDPASAHKID
                     HSAWDNWLKKYLVAPHPSGIHRVRYGSVTPEDRKALKNYLENLQKVDISTYNRAEQKA
                     YWINLYNALTVDVVLSHYPVSSIRDIGISPGLFSRGPWDAKLLTVETETLSLNDIEHR
                     ILRPIWKDARVHYAVNCASLGCPNLQPAAYTGGNTESLLENGAREYINHSRGIANRNG
                     KLQVSSIYDWFKDDFGASHDRLLEHWLKYADGDLAGALKTYKGSLAYGYDWSLNGADA
                     KP"
     gene            8812..9489
                     /locus_tag="VF089_15070"
     CDS             8812..9489
                     /locus_tag="VF089_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6175326.1"
                     /translation="MDISVIIPALNEEKTIAATLTALMALKPQEVIVVDGGSRDRTRE
                     ISEQMGAVVITGAPGRARQMNRGAGEATGDILLFLHADTRLPPAATDDVVGALQNPRY
                     LGGRFDVDLDGDHWLLKIIGAMINHRSRITKVGTGDQAIFVRREVFARMGGFPDIPLM
                     EDIAFCRALKRLGGIACLRSRVLTSARRWETEGVWRTVIKMWILKLLYFAGISPFTLK
                     RFYADAR"
     gene            9618..10310
                     /locus_tag="VF089_15075"
     CDS             9618..10310
                     /locus_tag="VF089_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6175327.1"
                     /translation="MSFFQQRANALVILTKAPQPGQSKTRLVPPLSYAEAAELARALL
                     LDQLESLTTFRGARLFIAFTPETAAGFFEGFISQGFTSFAQRGGSLGERMSHAFEHLF
                     ASAFENVILIGSDLPTLPVKFFHQAYAWLEKSADVVLGPSADGGYYLIGMKRMTPAIF
                     ADIAWSSADVLPQTIHKLDNLGLRHELLSEWYDIDTAKDLERLQSQRMNSEVAMKNTF
                     ALLNELTHRGRL"
     gene            10580..12628
                     /gene="tkt"
                     /locus_tag="VF089_15080"
     CDS             10580..12628
                     /gene="tkt"
                     /locus_tag="VF089_15080"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6175328.1"
                     /translation="MQDETDALCVNTIRMLSVDAVEKAKSGHPGAPMGLAPVAYVLWT
                     RFLKHSPKNPSWPDRDRFVLSAGHGSMLLYSMLHLTGYDLPLDQVKQFRQWESLTPGH
                     PERELTPGVEVTTGPLGQGFGNGVGMAIAEAYLAARYNRPGFEIINHFTYAIVSDGDI
                     MEGVAAEAASLAGHLKLGKLIYLYDDNRISLAASTDLSFSEDVAKRFDAYGWHTQSVG
                     DGNDLNAIERALAQARSESNRPSLILVRTHIGFGSPGKQDSFEAHGSPLGAEETKLTK
                     QKLGWPPEPFHLPEQALARFRDSINKGERFEANWNEQFTAYKKEFPELGRELEQALRG
                     ELPAGWDAAIPDFPADSKGLATRAASGKVLNAIAAKLPNLIGGSADLNPSTFTALSNL
                     GDFENPQGDFSKSQGLTGGGCNYAGRNLHFGVREHGMGAAVNGMAAHGGIIPFGSTFL
                     IFSDYLRPSIRLAALMELGVIYVFTHDSIGVGEDGPTHQPIEQLAALRAIPRLVLIRP
                     GDANETAVAWSVAIETRNRPVALALSRQNVPTLDRAKYTAAAGLRRGAYVLADPLQGR
                     PDLVLIGTGSELSLVVAARDKLLEQKINARVVSMPSWEIFDDQPQDYREFVLPPDIRA
                     RLAVEAALPLGWHRYVGDGGDVLAIERFGASAPGNIVMEKFGFTVDHVVERARSLMGK
                     "
     gene            12631..13101
                     /gene="rpiB"
                     /locus_tag="VF089_15085"
     CDS             12631..13101
                     /gene="rpiB"
                     /locus_tag="VF089_15085"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX6175329.1"
                     /translation="MRVAIGADHAGFQMKQVISEHLRSAGHAIIDVGTESDAPVDYPD
                     YAEALGNVLVEGKAERGVLICGSGVGASVAANKIPGIRAGLCHDSYSARQGVEHDDTN
                     VLVLGARVIGSALAKELVDRFLAAQFSREERHRRRVKKIKALEQRYQKSQGESR"
     gene            13098..>13679
                     /locus_tag="VF089_15090"
     CDS             13098..>13679
                     /locus_tag="VF089_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX6175330.1"
                     /translation="MNPLRELLNQGQSVWLDFISRQLIRSGELKRLVEEDGLRGVTSN
                     PTIFEKAIGGSADYDESLREMLAQDRKTPVGQLYEQIAIQDIQAAADVLRPVYDQTSG
                     ADGYVSLEVSPHLARDTQATIAEAKRLKASVDRPNLMIKVPATREGIPAIEELIAGGV
                     NVNITLMFSMSHYEAVAHAYIKGLERCADPAEVA"
BASE COUNT         2945 a         3763 c         3904 g         3067 t
ORIGIN      
        1 tgatgtcggg caatcgccat gaccgctccg cgttgggaag gattttcttt cgatcatcgt
       61 tgagagggat tcgctctgta ttgatgcagt ttattttcgc aattcaaaga gctgcgtgtc
      121 cggatagtga tcgatccaac caaaccagaa ggagggggta atcggtaacg cctcgagcct
      181 gcttcctttg agcgagccct gcaccgcttg cccggtcagt ccttgccaga tcgaaccact
      241 ttcgtcgtca accaaaaaca gccccttttc gctcttgagc tgagcttttt tgaatgaaag
      301 ggtctttcca tcaagctttc ttgtaaaaat cactccggtg gcggtctctt cgtcaaagac
      361 aatcaggagc gggactgcgg caaactcatc attgaccacc gatcgccggc tcagaactga
      421 ataggggtag gctttggcct tgccattcag gattaacccc aggacatact ccttgggata
      481 aatccttttg tctgattggc gggtcggaac aatgccagtg tcggcagaac ggtagtaaga
      541 ctcataggga tctcgtgcac tggtgagaaa tccaccgcga tcgcgggaga gaactttgga
      601 attggggtgg agttttttcc acgtgctcca tcgagtgaac atggactgaa tcggtttgag
      661 cttttctccc tttagcggac cggtcacggc ggcgccaacc agatggctcc agagactatc
      721 ggtctggtga tcgtacatga ttagcgagtt cgcatgaagt ttgcctgaga cgccgaaggt
      781 tagctcatga tccttaattc tacggtcata cacgatggcc gtatagcaga gcggtcacca
      841 agtcacggcg atcgggacac ctccaagttt atcattgaca atttcgtgcc tgctcaatat
      901 cgaaaccgga taggcacggc tttcgccggc aatgctcata cccaccacaa cgttatcgtg
      961 acgaagccag gaggccttcc cggcttcctc gaaggatggc gacatgatcg ctggaatggc
     1021 atctttgggt aaaaccgtga caatatcact ggggcggaga tcggcgcttc cggcagccgc
     1081 ggtgataact ccggctaata tcagcatcat caatccagcg gctaatccgt ttatccgcag
     1141 catcttcaag aatccttttg ttttctgccg ggtcatgccg tttgtcagca aagcgactcg
     1201 ctgtctagca ttcatgatcg ttcattatta gtgaccggca ccggtcattc gaatatctaa
     1261 ccaatttccc gtgcttttag ttcccgtgcg ggctgccgcg ttactcccca atgcggcaaa
     1321 aatacgaaat cgccaaattt ctggaacttt ttccttcata aacggttttt ggattgagat
     1381 cacataaagt gaaagatacg tgaccgattc caatctaatg agaattaaaa gcgccctcgc
     1441 cgcgttgatt gttctgttgt cggctgccgt gtggatttac ggtagagact cgagcccgca
     1501 gcctgcagct caaacgccgg ccacccatct ccttgccgat ctgggtatcg aaccgagcca
     1561 agaaagaacg ccggcccctg cttttctact caatgacagc gacggcaatg tggtgcgtct
     1621 tgacgatcta cgaggtaagg tggttttgtt gaacttctgg gccacctggt gcccaccctg
     1681 tcgcctcgaa atgccttcgc tggaggcgct tcataggcaa ctgggaccgc gaggtttagc
     1741 ggtgcttgcc gtcgcgagcc gagacaacgc cggggatgtc cgatcttttt tcaacgagca
     1801 tcgcctttct cttccggcac tgttggaccc aggtgcgcac gcgtacggtg tttacgaaat
     1861 ctggtcgctt ccgacaagtt tcgtgatcga taaacgagga tatcttgtcg gtaaagtcgt
     1921 cggccaccgg gattggatta gtgaacagag caaagatttc tttctgcatc tactgggagg
     1981 ctcaacttac atcttttaat cctaatcaac tcctcgtttc tgcgctgatg cctttggaga
     2041 tattttattg gcggcgcagc gagataatct ccaccctatc gaccagaaaa ggagaagcag
     2101 caaggatatg gacgcacttt caagaatcaa tggagccaac ggcaaagtcg cggttaccga
     2161 ttttgaagca gcggtgctgc agcgttacgg cgacgccgct caggaagtcg agcagtgtct
     2221 ttgcctgccg gtaagttacg acaagggttt gcttggcgtc atccctcagg aaatcatcga
     2281 caaagattat ggctgcggcg atccatctcg ttatgttcgg caaggcgaaa ccgttttaga
     2341 tctcggttcc ggcagcggca aagcctgcta cataatcgct cagattgtcg gcccggaagg
     2401 caaagtcatt ggtgtagatt ttaatccgcc aatgctggat ctcgcgcgta agtatcaaga
     2461 gtcgatcggc gaccgcctcg gttatcgtaa catcgagttt cgccgcggca aaatccagga
     2521 tctaaaaacc aacctcgaat tggtcgaagg ctatctccaa gctcacccgg tagtctcggt
     2581 ggccgatcta gcgaagctgg aaatctatga agatcacatc cgtgagaaac agcctttgat
     2641 tgccgacgac tctatcgacg tgatcgtttc aaactgcgtg ctcaatctgg tccggccgaa
     2701 tgacaagcga cagctgttta ccgaaatgta ccgcgtgctc aaacggggcg gccgagtggc
     2761 aatctccgac attgtctgcg atgagccggt gccggagcat ctggtaaacg atccgaacct
     2821 ttggagcgcc tgtgtttccg gcgcttttca ggaagaagaa tttatgcgcg cctttgagga
     2881 agccgggttc cacggcatcc gcatcgaaga gttgcgcagc gaaccctatc agaccgtcga
     2941 aggcattgag tttcgcgcgg ttacattgac cgcttacaag ggcaaagagg ggccgtgtat
     3001 cgagcgcaac cacgcggtca tttaccgcgg cccttggaag caagtggtcg atgacgatgg
     3061 ccacaggctg gagcgtggcg cgcgcatggc ggtatgcgac aagacattca agctctattc
     3121 gcagccgcct tatgccggcg aatttattct gctgccgcca cgcgaggaag tcgcggcaga
     3181 agcagcaggc ttgttcgatt gcgcgcggga ccatcggcgc cacccgcggg agaccaaggg
     3241 cgaggacttc aaagtcactc gggtatccgg cagcgtttgc ggtcccggct ccgattgctg
     3301 cccatagcgg agttagcatg caagcttttg tcgataagat tcgctcatac ggcggcgact
     3361 tcagccggct cgcggtcgac gtccttcaag tcaacatggg ccgttactgc aatctggctt
     3421 gccttcactg ccatgtcgaa tcgggtccga ctcgtacaga gatgatgagc cgggagaccg
     3481 tcgatgctgt actgcgattt ctcgccagct cgaatattcc gactctcgac atcaccggcg
     3541 gcgcgccgga gctgcatccc aattttgatt acttggtcac ggcggtgcgc caactcaaac
     3601 ggcacgtgat ggaccggtgt aatctcacgg tcatctttga acccggcaag gagtatctgc
     3661 cagagttttt caaggagcac cgtgtcgagc taatttgttc gctaccctgc tattcccaag
     3721 aaaatgtcga taagcagcgc gggaaaggaa ccttcgatgc cagtattcgc gcactgcaac
     3781 gtctcaacca aatcggctat ggcaacacgg acggcgatct ggtcttgaat ctcgtctaca
     3841 atccagtggg cccgcacctg ccgccgccgc aacagaaact cgagcaggac tacaaacaaa
     3901 tcctcgccga aaacttcgga attgccttca atcatcttta ctgcctgacc aatatgccga
     3961 tcacccgcta tggcacgcat ctcaagctgc ggggcgagta cgatcgctat cttgatttat
     4021 tggcaaccaa tttcaacggc gcgaccttgg atcaagtgat gtgccgcaat ctcatcagcg
     4081 tcggctggga aggcctgata tacgattgcg atttcaacca gatggtcaat ctgccgattc
     4141 gagaccgagc cggcaagccg cttcacatca gctcgctatc gatcgaacag ctgcgcaatc
     4201 acgcaattac ggtcggcgat cactgctatg cctgtaccgc cggcgcgggc agcagttgcg
     4261 gcggcacgct ggtttagaac ccgaaagcaa gaatgattga ctggtcgatc atcatagccg
     4321 gagcgacgtt ttttgtcggc tacgccaacg gtgccaatga taatttcaaa ggcgtcgcaa
     4381 ctctctttgg cagcggcata acggagtacc gcaaagcgct tgcctgggcg acgatcacga
     4441 ctctggccgg atccgtagcg gcgttctttc tcgccaccga gctgatctca atcttccaag
     4501 gcaagggatt ggtgccgccg gagctctcca actcggagcc gttcgtggct gcggtggttc
     4561 tgggcacggc gctaactgtc atgcttgcga ctaaactcgg tatgccgatt tcgactacgc
     4621 acagcttgac gggcgcgcta tgtggcagcg gcctggtcgc ggtcggcttc ggtttcgggt
     4681 tcaaagcgct cgcagtaaat ttcttcttac cgctgttggt cagcccgttc tgtgccgtag
     4741 cgattgcaaa cttcgcgttt cctttgctga accgcctcgt gcagcgcgcc gctctcaacc
     4801 aggaaaattg tctttgcgtt gaggcggtgg cgccatcgac ggtaactccg gaaggtctgg
     4861 tattattcca aaccacgcct gggttacgtg taatcatgga tcgagaagcg gtctgcgcgc
     4921 agcccggctc accgacaatt ctcggtatca acggccagaa gttactcgat gcaagccatt
     4981 tcttgagcgc cggtgcggtc agcttcgcgc gcggtctcaa tgacacgccc aagattgtcg
     5041 cgcttagtct ggtcgccgga gcgtttgatc tgcgcttgtc caccttgctg gtcgctgcga
     5101 tgatagcgct cggcggccta ttcaacgcca gagaggttgc ccagacagtc agtaagagaa
     5161 taaccgggat ggaacccgtt catgggcttt gggctaatct ggttactagc tttctggtta
     5221 tttttgcctc caagtggggc atgccggttt cgaccacgca tgtgtcttgc ggcgcgctct
     5281 tcggcatcgg tatcgccaac ggccaggcac gctggaaaac gatccgcaca atactcatgg
     5341 cttggcttgt gacattgccg ctggcggcgc tgctgtccgc cggcgtcttt gttgtattcc
     5401 ggcagttgtg accttcttgc tctccaggca aattcgattg catcctccgg gctaaggttt
     5461 gtgtctaagt aagatgagtc gagggatcta gatcagcaaa cgagatgaag acaagcaaga
     5521 cgatcgtcgt tattttaatg ctatcggcaa tcatagccgg tgttctgttt ctgccggtgc
     5581 gccagtggtt cattcacttc gaaggctacg tgcaatctct gggtgctatc gggcccgcgg
     5641 ccgtggtgct ggcgtacata ctgtccacgg tgctgtttat tccgggaagc gcgctgacca
     5701 tcggttcggg aactctcttt ggcttgacga caggatttgt tgtcgtattt ttgggcgcca
     5761 acctcggcgc gctcggttct ttcctactgg cgcgaaccct tttgcgggat aaagtcgctc
     5821 gctgggcgga ggccaatcca aagtttcgct ctctcgatca ggcgatcggc aagcaggggt
     5881 tcaagatggt cctgctgacg cgtctgagcc cggtgtttcc ttttgtcctg ctgaattact
     5941 tcctcgggct cactgcggtg cgcaccgggt cctatgtcct ggctaacgtc atcggcatgc
     6001 tgccggcaac tttccttttt gtttacatcg gcgcggcggc gcgggatgcc ctggccggtc
     6061 agatggatgc ttcggccgga ttctaccagc agattctaaa agtcgtcgga ttgctggcga
     6121 ccgtcgcggt cgtggttgtc gtcacccgca tcgcgcgcaa agcgttgcgc gaagccgagc
     6181 aggaacaggc aggcgatggg ggtcgtgagc cgcatctcaa tacgaaccac cagccggcat
     6241 cttttgaaac gatggtgctc atcgatgacg aacacgaccg gcggctaata gagaactgtc
     6301 acccacctcg gtgggtcaac ccggcacctg ccgcgaagta taatctagtc gtcattggcg
     6361 cgggcactgc cggtctggtc agcgccgctg gcgccgcggg actcggcgcc aaggtcgctt
     6421 tgatcgagcg caatctcctg ggcggcgatt gtctgaacgt cggttgcgtg ccgtcgaaag
     6481 ggatgatccg ggcggcacgc gccgcgcatg atgcgcgtga gggagcgaaa ttcggcgtcc
     6541 ggcttacttg cgagccagag gtcgggttcg ccgccgcgat ggagcggatg cgcaagcttc
     6601 gcgccggcat cagcgatcat gattccgccg aacgctttcg caagctcggc gtcgatgttt
     6661 tcattggcag cgcccgcttc accagcccgt cttcaattga tgtggacggt atacgccttg
     6721 attttaatcg tgccgtcatc gccagcggcg ccagggcggc ccaactgcca gttccaggcc
     6781 tcgacgaagt tggctatctt accaatgaaa cggtttttac attgactgag ttaccgcggc
     6841 gaatcgcgat catcggcgcc ggccccatcg gctgcgagct ggcgcaagcc ttcgcccgct
     6901 tcggcagcaa ggtttctttg ctaaccgacg gcgcggacat attgcccaag gaagatcgcg
     6961 atgcggcagc gattgtacgc aagcaaatgg aacatgatgg cgtgcggatc gtaactggcg
     7021 ccaagattca ccgcggcacg aaaaacaagc aggagaaaac gctcgctgtc gcaattgacg
     7081 ggtcgacagt cgaactcggt tgcgacgcga tccttgtggc cgtggggcgc acacccaatc
     7141 tcgaaggact ggacctggaa gcagccggca taagctacac gaggagagga gtcgacgtcg
     7201 atgaccggct ttgcactacc aatccgcggg tctttgcagc cggcgatatt tgctcgcgct
     7261 ataaattcac ccacgccgcc gacgccatgg cgcgcatcgt aatcgccaac gctctcttca
     7321 tggctcgacg gaaggtaagc ggcttaacga ttccctggtg cacttacacc gatccagaga
     7381 tcgctcacgc gggttactac gagaaggacg cgcaagcggc agggttcaat gtcgcgaccc
     7441 ttaccgagtc gctcgatcat gtagatcggg cgattctcga cggcgaagaa gaaggcttcg
     7501 cgcgggttca ctacgacaag aaaaccggga aaattctggg cggcacaatc gtggctcgac
     7561 acgccggcga gatgatcagc gaactgacgc tggccatggt ggcaaagcaa ggcgtcggag
     7621 ttttatcctc gaccattcat ccgtatccga cacaggctga ggcgtggcgc aaaatcggcg
     7681 atgcttatat gcgcaccaag ttgactccga gagtgaaaag ggttttcgaa aaatggctgg
     7741 cgtggcgcag atgacgtctc gagcaaagtt tctttggcgg ggtctcctgc ggcttgagcc
     7801 taccaggttg ctgaaaggct ctgtttgcag gctgcgcttt ttttccggcg tggcgctgct
     7861 ggctatcttc ggcgcccctc aggcggatct ctggccgaaa tggcaaaagc acgatcccgc
     7921 cagcgcgcac aagatcgatc acagcgcctg ggataattgg ctaaaaaagt atttagtggc
     7981 gccgcatcct tccgggatcc atcgagtccg ttacggctcg gtgacgcccg aggatcgaaa
     8041 ggcgctaaaa aattacttag aaaatcttca aaaggtggac atttccacgt acaaccgagc
     8101 ggagcaaaag gcttattgga tcaatctcta caacgcgctg acggtagacg tagttttgtc
     8161 ccattatccg gtttcatcga tcagggatat cggtatctct ccgggactat tcagccgcgg
     8221 gccgtgggat gccaagctac tcaccgtcga aacagaaaca ctttccctta atgacatcga
     8281 gcaccgcatc ctgcggccga tctggaagga tgcgcgtgtg cactacgcgg tcaactgcgc
     8341 cagcctcggt tgccctaatc tccagcctgc ggcctatacc ggaggcaata cggaatcgct
     8401 gcttgaaaac ggcgcccgcg aatacatcaa tcactcaaga ggcatcgcca atagaaacgg
     8461 caagctccaa gtttccagca tctacgactg gttcaaggat gatttcggcg ccagccatga
     8521 tcggctcttg gaacactggt tgaaatacgc agacggagat ttggccggcg cactcaagac
     8581 ctacaaaggc agcctggctt atggctatga ttggagtctc aacggggcgg acgccaaacc
     8641 ttaaccaatg cggagaacct agcgtggcaa agccgcaact aaaggagcta gaaagggaca
     8701 acccatttct accacgaagg tctcggaccc cacctctttc cttccccgcg tcacgcgggg
     8761 gaggatgaag gagggggaag ttcgaatcgc ataagaacta tagcgcttca gatggacatc
     8821 tccgtcatca ttcctgcgtt aaacgaagaa aaaaccattg ccgcgacttt aaccgctttg
     8881 atggcgctta aaccgcagga ggtgatcgtt gtcgacggcg gcagcagaga ccgtactcgc
     8941 gagatcagcg agcaaatggg tgccgtggtt attaccggcg cgccgggccg agcgcgccag
     9001 atgaaccgcg gcgctggaga agctaccggc gatattctgc tgtttctaca tgccgacacc
     9061 cgtctaccgc ccgccgctac cgatgacgtt gttggagctt tacaaaatcc gcggtatttg
     9121 ggcggtcgct tcgatgtcga tctggacggc gatcactggc tgctgaaaat aatcggcgcc
     9181 atgatcaacc accgctcgcg cattaccaaa gtaggcaccg gcgatcaggc gatttttgtc
     9241 cggcgcgaag tgtttgcacg catgggcggc ttcccggata ttccgttgat ggaagacatt
     9301 gccttctgtc gagcgctaaa gcggctgggc ggcattgctt gcttgcgcag cagggtcctg
     9361 acttcggcgc gccgctggga gaccgaaggc gtgtggcgga ccgtcatcaa gatgtggatc
     9421 ttgaagctcc tctacttcgc cggaatttca ccgttcactt tgaagcgttt ttatgccgat
     9481 gcccgctaac atttcgctga agtctgaatg caggctgctc aaaaaggatc tcagaggcaa
     9541 ggcgcgcgaa gaatcgatga gctgaggcgt acggtcggcc gtacgtcgaa gcgagacgaa
     9601 caagcgtaac gaggcatatg agtttttttc agcaacgtgc taacgctctg gtgatcttga
     9661 ccaaggcgcc gcagcccggc cagagcaaaa ctagactggt tccgccgctg tcctatgccg
     9721 aagccgccga gcttgcgcgg gcgctgctgc ttgatcagtt ggaaagcctc acgaccttcc
     9781 gcggcgcacg cttgttcatc gcgttcacac ctgaaacagc tgccggcttt ttcgaaggtt
     9841 tcatttctca gggctttaca tcctttgccc aacgaggtgg atcgttgggc gagcgcatga
     9901 gtcatgcatt cgagcatcta ttcgccagcg cttttgaaaa cgttatcttg atcggcagcg
     9961 atcttcctac gcttccggtg aaattcttcc accaggctta cgcgtggttg gagaaatcgg
    10021 cagacgttgt gctcggcccc agcgccgatg gaggttatta cctgatcggc atgaagcgga
    10081 tgactcccgc cattttcgct gacatcgctt ggagcagtgc cgatgttctc ccgcaaacga
    10141 ttcacaaact ggacaatctg ggcctcagac acgagcttct ctccgaatgg tatgatatcg
    10201 atacagcaaa agatctggag cgcttgcagt cacaaagaat gaacagtgaa gttgcgatga
    10261 aaaacacctt tgctctattg aacgagctta cacaccgagg cagactataa ttagcaggtt
    10321 gctgaaaaag cctcagatgc ttcgttgcgc tcaatcgact cgcatcaacg taccgagtaa
    10381 gtacgcctct gctcgtccat tttcgcgcgc ctcgcatctg aggctttttg aacagcctgt
    10441 aatcagagtt aatcagcgat ctgctctaga gcttagcaag ctctaaagct ctggttctgg
    10501 tggtcccacc ctccgttttc cagcttgata attttcgtcg gcgcggctgt gtccgtggcg
    10561 gcggcgagga gaactagcga tgcaagatga aaccgacgcg ctatgcgtca acacgatccg
    10621 catgctgtcg gtggatgccg tggaaaaggc gaaatcaggt catccgggcg ctccgatggg
    10681 gcttgcaccg gtagcttatg ttttatggac ccgcttcctt aagcatagtc cgaaaaaccc
    10741 gtcgtggccg gatcgtgacc gtttcgtttt gtcagcggga catggctcga tgctgcttta
    10801 tagcatgctt catcttactg gttacgatct gccgctcgat caagttaaac agttccgcca
    10861 gtgggaaagt ctcacgcccg gccatcctga acgcgaactg acacccggag tagaagtcac
    10921 caccggaccg ctcggacagg ggttcggcaa cggcgtcggc atggcgatcg ctgaagccta
    10981 tttggcggcg cgctataacc gtccggggtt cgagatcatc aaccatttca cctatgccat
    11041 cgtcagcgac ggcgatatca tggaaggcgt cgccgcagaa gccgccagcc tggcggggca
    11101 tctgaagttg ggaaagttga tttatttgta tgacgataat cggatttccc ttgccgcgtc
    11161 caccgatttg agctttagcg aagatgtcgc caaaaggttt gatgcctacg gctggcacac
    11221 gcaatctgtt ggcgatggca acgatctgaa tgcgatcgag cgtgctctcg cgcaggctcg
    11281 cagcgagagc aaccgcccgt cactgatttt agtgcgcacg catatcggct ttggttcgcc
    11341 ggggaagcaa gactccttcg aggcgcatgg atcgccgcta ggcgcagagg aaacaaaact
    11401 gactaagcaa aagctcggct ggccgcctga acccttccat ctgccggaac aggcgctggc
    11461 gcgtttccgc gactctatta acaaagggga aagatttgaa gcgaactgga acgagcaatt
    11521 cactgcttac aagaaagaat ttcccgaact aggaagagag ctggagcaag cgctgcgcgg
    11581 cgaactaccg gcggggtggg acgccgcgat tccggatttt cccgcggact cgaaaggttt
    11641 ggcgacccgc gccgcctcgg ggaaagttct caatgccatt gccgccaagc tgcccaactt
    11701 gattggcggc tctgccgatc taaacccgtc gacgttcact gcactgagca atctgggtga
    11761 cttcgaaaat ccccaaggcg atttctcgaa gagccagggc ctgacaggcg gaggctgcaa
    11821 ttacgccgga cgcaacttgc actttggggt gcgcgagcac ggcatgggcg cggcggttaa
    11881 cggcatggcg gcgcatggcg gcatcatccc attcggttcg acgttcctga ttttctccga
    11941 ttacttgcgc ccttcaataa ggcttgccgc tttgatggag ctcggcgtga tctacgtatt
    12001 cacgcacgac agcatcggcg tcggcgagga cggcccgaca catcaaccga ttgagcaact
    12061 cgccgcgtta cgcgcgatac cgcgcttggt gctaattcgt cccggcgatg ccaacgaaac
    12121 cgccgtagcc tggagcgttg ccatcgagac ccgcaatcgg ccggttgcgc tggcactgtc
    12181 gcggcagaac gtgccgacgc tcgatcgtgc gaagtacacg gcggcagccg gcttacgccg
    12241 gggcgcctat gttttggctg atccactgca gggcagacca gacctggtac tgatcggtac
    12301 cggatcggaa ttgtcgctag tggtagccgc ccgggacaaa ctgctggaac agaaaatcaa
    12361 tgcgcgggtt gtttcgatgc ccagctggga gatctttgat gaccagccac aggattatcg
    12421 tgagttcgtc ctgccgcccg atatccgcgc gcggctcgct gtcgaggccg cgcttccgtt
    12481 aggttggcat cgctacgttg gcgatggcgg tgatgtgctt gccatcgagc gctttggcgc
    12541 gtcggctccg ggaaatattg tcatggagaa atttggtttc accgtcgatc acgtcgtcga
    12601 acgcgcccgc tcgctgatgg gaaaataacc atgcgcgtgg ccataggcgc cgatcatgcc
    12661 gggtttcaaa tgaagcaggt catcagcgag cacttgcggt cggcaggcca cgccatcatc
    12721 gacgtcggca ccgagagtga tgcgccggtc gattatccgg attatgccga ggcattgggc
    12781 aatgtccttg tcgaaggcaa ggcagagcgc ggcgtgctca tttgcggtag cggtgtcggc
    12841 gcttcggttg ccgccaataa aatccccggc attcgcgccg gcctctgtca tgacagctat
    12901 tccgcgcgtc aaggggtcga gcatgatgac acaaatgttc tggtcctcgg ggctcgggtc
    12961 atcggcagtg cgctggcaaa ggagctggtc gatcgttttc tggcggcgca atttagccgc
    13021 gaagagcgcc atcggcgccg ggtcaaaaag atcaaagcgc tggaacagcg ctatcaaaaa
    13081 tcccaaggag agagtcgatg aatcctttaa gagagctttt gaatcaaggc caatcggtat
    13141 ggctcgattt catcagccgt cagttaatcc gaagtggcga gttgaagcgt ctcgtggaag
    13201 aagatgggct gcgtggtgtc actagtaatc cgacaatctt tgagaaagcg attggcggca
    13261 gcgcggatta cgacgagagt ttgagggaaa tgctggctca ggatcggaag actccggtcg
    13321 ggcagttgta cgaacagatc gccattcaag acatacaggc cgcggccgac gttcttcgtc
    13381 ctgtttacga tcagaccagc ggcgccgacg gatatgtcag cctcgaagtt tcgcctcatc
    13441 tagcgcgcga cacgcaagcg acgattgcgg aggccaagcg gctcaaggct tcggtcgatc
    13501 ggcccaacct tatgattaaa gttccggcta cccgcgaggg aattccggct atcgaggagc
    13561 tcattgccgg cggcgtcaat gtaaatatta cattgatgtt ttcgatgtcc cactacgaag
    13621 cggtggcgca cgcctacatc aaaggcctgg aacgttgcgc cgacccggcg gaagtggct
//
LOCUS       DASUTG010000652         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1731441, whole genome shotgun sequence.
ACCESSION   DASUTG010000652 DASUTG010000000
VERSION     DASUTG010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731441"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2060)
                     /gene="ppsA"
                     /locus_tag="VF089_15095"
     CDS             complement(<1..>2060)
                     /gene="ppsA"
                     /locus_tag="VF089_15095"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX6175331.1"
                     /translation="NIDQQIQEILSGLDTRDTANLQQRGSAVRHAIMAALLPQDLQHS
                     IIAAYRELSRGADKPMDVAVRSSATAEDLPNASFAGQQETYLNVQGDAALLESCKRCF
                     ASLFTDRAISYRQDKGFDHFKIALSIGVQRMVRSDLASSGVMFSIDTETGYQDIVLIN
                     AAFGLGENVVQGSINPDEYFVFKPTLKQGFRPILQKRLGSKEFKLVYDIGGSKMVKNI
                     PVAPDDRSRFAITDDEILCLARWACLIEDHYSQQRGQYTPMDMEWAKDGYTGELFILQ
                     ARPETVQSQKSSDRVEVYHLQEKRPALVRGRSVGEKIAHGPVRVVKNAHNLNQVQKGD
                     VLVTDKTDPDWEPTMKKAAAIVTNRGGRTCHAAIVSRELGLPAIVGTENATEILKDDQ
                     AVTVSCAEGETGFVYQGLLKFDVQQIKLHDLSRPQTQVMMNVGNPEEAFRLSSLPNDG
                     VGLAREEFIISNYIKVHPLALLNYDRIEDQALREQIAQLTIGYDDKPQFFVDKLAQGV
                     AMIGAAFYPKDVIVRLSDFKTNEYANLIGGKAYEPVEENPMIGFRGASRYYNPRYQAG
                     FALECRAMKKVRDEMGLSNVKLMVPFCRTVEEGRRVLEEMEKHGLKRGDNKLEVYVMC
                     EIPSNVILANEFAEIFDGFSIGSNDLTQLVLGVDRDSEIVAHVFDERNPAVKNMIASV
                     IQAA"
BASE COUNT          425 a          553 c          561 g          521 t
ORIGIN      
        1 ttggcggctt ggatgacgct agcgatcatg tttttcacgg ctggattgcg ctcgtcgaaa
       61 acatgcgcca cgatctccga gtcgcggtcc actccgagga cgagctgggt gagatcgtta
      121 gagccgatgg agaagccatc gaagatctcg gcgaattcgt ttgccagaat gacgttgctg
      181 gggatttcgc acatgacata gacttccaat ttgttatcgc ctcgtttcaa tccatgcttt
      241 tccatttctt cgaggacgcg ccgaccttct tccaccgtgc ggcagaaagg aaccatgagc
      301 ttcacgttgc tgagtcccat ctcgtcgcgc acttttttca tggcgcggca ttctaaagca
      361 aagcccgcct ggtaacgcgg attgtaataa cgtgaggcac cgcggaagcc gatcatcggg
      421 ttctcctcga ccggctcata ggccttgccg ccgataagat ttgcgtattc gttggtcttg
      481 aagtcgctca gacgcacaat gacatccttg ggataaaaag cagcgccgat catagccacg
      541 ccttgcgcca gcttgtcgac aaaaaactga ggtttgtcgt catagccgat cgtgagttgt
      601 gcaatttgct cgcgtagagc ctggtcttcg atgcggtcgt agttgaggag agccagtggg
      661 tggaccttga tgtagttcga aatgataaat tcttctcgag ccaaaccaac gccatcgttt
      721 ggaagcgagg agagacgaaa agcctcttct ggattgccca cattcatcat cacttgagtc
      781 tggggccgtg acagatcgtg aagttttatt tgttgcacat caaatttgag caaaccctgg
      841 tacacgaatc ccgtctctcc ctcggcacaa gaaaccgtga cagcttggtc gtcctttaaa
      901 atttcggtgg cgttctccgt cccgacgatt gccggcagtc caagctcgcg gcttacaata
      961 gcggcgtggc aggtccggcc gccacggttc gtgacgattg cagccgcctt cttcatggtc
     1021 ggctcccagt ccggatcggt cttgtcggtg accaatacat cgcccttctg aacttggttg
     1081 aggttgtgcg cgtttttcac tacgcgcacg ggtccatgag cgatcttttc tcccacgctg
     1141 cggccgcgca ctagcgccgg cctcttttct tgcagatgat aaacctcgac cctatccgac
     1201 gatttctgcg actgaaccgt ttcggggcgc gcctggagaa tgaagagttc ccccgtgtat
     1261 ccgtccttgg cccattccat atccattggc gtgtattgac cccgttgttg actgtaatga
     1321 tcttcgatga ggcaggccca tcgggcaaga cataggatct cgtcatcggt aatggcgaag
     1381 cgagaccgat cgtcaggtgc gaccgggatg ttcttcacca tcttacttcc gccgatgtcg
     1441 tagactagtt taaattcttt gctccctaag cgtttttgca ggataggccg aaagccctgc
     1501 ttgagcgtgg gcttgaagac gaaatactcg tcgggattga ttgaaccttg gaccacattc
     1561 tcgccgagtc caaaggcagc gttgatcagt acgatgtcct gatagccggt ttcggtgtct
     1621 atggaaaaca tgacccccga cgacgcgaga tcggatcgga ccatgcgctg aactccaatg
     1681 gatagcgcga tcttgaagtg gtcaaatccc ttgtcctgcc gatacgagat tgccctgtcg
     1741 gtaaagagag aagcaaagca gcgcttgcag ctttccaaga gggccgcatc accttggacg
     1801 ttgagatagg tttcctgctg gccggcaaag ctcgcattcg gcaaatcctc cgcagtggcg
     1861 ctgctccgga ctgcgacatc catcggttta tccgctcctc tgctcagttc ccggtaggca
     1921 gcgataatcg aatgctggag atcttgcggc agcagtgctg ccatgatggc gtggcgaaca
     1981 gcgctgcctc tttgttgaag atttgctgtg tcgcgtgtgt cgaggcctga aaggatttcc
     2041 tggatttgct gatcgatgtt
//
LOCUS       DASUTG010000653         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_101745, whole genome shotgun sequence.
ACCESSION   DASUTG010000653 DASUTG010000000
VERSION     DASUTG010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101745"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1002
                     /locus_tag="VF089_15100"
     CDS             253..1002
                     /locus_tag="VF089_15100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175332.1"
                     /translation="MKLNGKVAFITGFGSGLGQAIAVMFAKEGAAVAGTSTTESKGKE
                     TVAMIEKHGGKALFNPGDVGNSEQMQSLIAAAVREYGGLDIVVNSAGVRTNGSITEIS
                     EEAWDRTLDVNLKGAFVVSRLAIPEMKKRGGGVILHIGARSGMLGQAGRAAYCASKGG
                     LIRLTEAMAMDHAKDKIRVNCICPGPVRTPMVDTSTPEKLARYKTRVPLGRIGEPDDV
                     AYAALYLASDEASYVTAAILPVDAGMRLTGP"
     gene            complement(1039..1749)
                     /locus_tag="VF089_15105"
     CDS             complement(1039..1749)
                     /locus_tag="VF089_15105"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX6175333.1"
                     /translation="MRNKTVTTEYNEQHVTGSRRIWIATGVLVALLGMAAVWRWTPLA
                     DQIDIGKVTAWATALRGNPSRVMIILGAYLIGSIISLPITILILATALVFGPINGIVY
                     SFIGCMIGAAATYAMGYFLGSDFIRKLLGARWKTLERKINQTGILAVAAVRAIPIAPF
                     TVVNIVSGAFQVPVRDYLIGSLLGLAPGIILTNLFAHQLQSAIRNPGVGSLILLGVLV
                     IVSIFGTIWLRRKLANKD"
     gene            1983..>2490
                     /locus_tag="VF089_15110"
     CDS             1983..>2490
                     /locus_tag="VF089_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175334.1"
                     /translation="MSTAAALRTPESVLGDERIISADSHVMEPEDLWIRSLPPGLKEK
                     YPKFPVRNSPGEKPGGWNPRARIDEMETDGVSAEVLYPTLGLRLFALEDAELQEACFR
                     IANDWLIEYCHAVPGRLVGIPMISLYNIKNGIRELERCKKEGLVGCLIWQVPPEHLSF
                     TSDYYYPFW"
BASE COUNT          575 a          649 c          717 g          549 t
ORIGIN      
        1 aggcgccgtc ggcgcttagc cgaagaaggc ttggcgccgc tcccgagtgt atcaagtctt
       61 atcctggacg gccaaggata caaagagcaa tccgttaaag gtttttggaa gtcggcttgc
      121 cgtgcgtcct gaggcgctga cgcagatttc gctcgttgcg cgattttcac gtcacccatc
      181 acctcgagtg ccgcgagttg accgtgagaa acagtcgatt gtatgctcat caacttagga
      241 ggaacgtaca ttatgaaact gaacggcaaa gtcgccttca tcaccggttt cggttcaggc
      301 ctcggacagg ctatcgctgt gatgtttgcc aaagaagggg ccgccgtagc cggcacctcg
      361 acgaccgaat ccaagggcaa agaaacagtc gccatgatcg agaagcatgg aggaaaagcg
      421 ctgtttaatc ccggggatgt tggtaattca gagcagatgc agtcgttgat tgcagcggcg
      481 gtccgggagt acggcggact cgatattgtt gtcaacagcg ccggcgtcag aaccaacggg
      541 agtatcacgg aaatttctga ggaagcctgg gaccgcactt tggacgtcaa tctgaaagga
      601 gcgtttgtcg tttcgcgtct cgcaataccg gaaatgaaaa agcgcggcgg cggtgttatc
      661 cttcatatcg gtgcccgatc cgggatgctg ggccaggcag ggcgagcggc ttattgtgct
      721 tcgaagggcg gcttgatcag gttgacagag gcaatggcaa tggatcacgc caaagacaag
      781 atccgagtga attgcatctg cccgggtccc gtccggacac ccatggtgga cacatcgacg
      841 ccggagaaat tggcgcgcta caagacgcga gtcccgcttg gtcgcatcgg cgagcctgac
      901 gacgtggcct atgcagcgct atatctggca tctgatgaag cttcctatgt gactgccgca
      961 atcttaccgg tggacgctgg aatgcgcttg acaggaccgt gattttgaag gcattcgctg
     1021 ccgtgtattt cgccgtattc agtctttgtt cgcgagcttg cgtcttaacc agatggttcc
     1081 gaaaatgctg acgatcacca acacgcccaa caagatcaaa ctgcccactc ccggattacg
     1141 gattgcgctc tgcaactggt gtgcgaacag gttggtgaga attattccgg gcgcgaggcc
     1201 aagcagtgat ccgattagat aatcccgaac cgggacctga aaggcgccgg aaacaatatt
     1261 gaccaccgtg aacggcgcga tcggaatcgc gcgtacggct gccaccgcca agattcccgt
     1321 ttggttgatc ttgcgttcta acgttttcca cctagctccc aggagttttc ggatgaaatc
     1381 gctgccaaga aaatagccca tggcgtatgt cgctgctgcg ccaatcatac atccgatgaa
     1441 cgaataaacg attccgttga tcgggccgaa caccagggcc gtggctagga ttaaaatcgt
     1501 aatcggaagc gaaatgatac taccgatcaa ataggcgccg agaataatca tcacgcgcga
     1561 tggattgcct cgcagcgccg tagcccatgc ggtaactttg ccgatgtcga tctgatcggc
     1621 cagcggcgtc caacgccaca ccgccgccat tcccagaagc gcgacgagaa ctcctgtcgc
     1681 gatccagatt ctacgtgagc cggtcacgtg ttgttcgttg tattcggttg tcactgtctt
     1741 gttgcgcacg aagcctaccg tcaatcgtta aatgattaca attgccatgc cagccgacgt
     1801 aggaacaggc aatcgggtct tcgacagaag catgttggcc atgtctccgt cagcactcga
     1861 acggacggct cgaggcctgc catttcaacc gtccgagcca tcgtacggag ctgtgtcctt
     1921 tgttagacaa accgatttga ttggactaaa caagagacat cacggaaaga gaggtatcgc
     1981 taatgagcac agctgctgct ttgcggacgc cggaatcggt tctgggcgac gagcgcatca
     2041 tatcggcaga ttcccacgtg atggagccgg aggatttatg gattaggagt ttgccgccag
     2101 gtctaaagga gaagtatcca aagttccccg tgcgtaactc ccccggtgaa aaacccggag
     2161 gatggaaccc tcgggcacgg atcgatgaga tggaaaccga cggtgtctcc gctgaggtgc
     2221 tttatccgac tttggggctg cgcttgttcg cgctcgagga tgcggaactt caggaggcgt
     2281 gtttccggat cgccaacgat tggctgattg aatattgcca tgctgttccg gggcgtctgg
     2341 tcggcatccc gatgatttcg ctttacaaca tcaaaaatgg catcagagag ctggagcgtt
     2401 gtaaaaagga gggactggtc ggatgcttga tctggcaggt tccgcccgag cacctgtctt
     2461 ttaccagcga ctattactat cccttctggg
//
LOCUS       DASUTG010000654         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_991568, whole genome shotgun sequence.
ACCESSION   DASUTG010000654 DASUTG010000000
VERSION     DASUTG010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991568"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VF089_15115"
     CDS             complement(<1..389)
                     /locus_tag="VF089_15115"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX6175335.1"
                     /translation="MQALRISPAADRRAFFWLMGSLIAVAWLVIWIWDRSPYGRYLHH
                     AELGELGISASPFLAVVLYLLGWTLMTAAMMLPTSLPLLEIFRRLIMRRPDRSRLMIE
                     VITGYLSVWLLFGIAAHSADWIVHQAV"
     gene            complement(541..1143)
                     /locus_tag="VF089_15120"
     CDS             complement(541..1143)
                     /locus_tag="VF089_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX6175336.1"
                     /translation="MAYKLEGSLLEVCNCEVLCPCWIGVDPDNGTCDTVVAHHFDRGK
                     IDDVDVSGLTMGFTAHVPGNILKGNWRAVVHIDQAATKEQEEALLSVYTGKQGGPLAD
                     LFQLIGEVVAVERSPITFKVEEGKGTLRIGNVADCEMEPFRGPNGDVTTLHDSVFSTI
                     PGSPAYVAKATKYKMDNPKLGQSIDLKDHNAIQGKFVFEG"
     gene            1587..2582
                     /locus_tag="VF089_15125"
     CDS             1587..2582
                     /locus_tag="VF089_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175337.1"
                     /translation="MKSTLILSALAVFLLLPQRAPGQEQLKLIYSQFTMTNSATWFAR
                     EAGLFERHGLNADLIYVDSAPAVQALTAGAAPLATMSGGLAVGPYLNGLDLVMLAGWC
                     NLNSYQLITRPEIRRPEDLRGKVIGIGRYGAAADWALRLILRKLGINEHKDVQIIQAG
                     GGGPATRLGAMEAKKLDATVLDSPQTVQARRLGFRTLADGVELGIPFLQGGLVTRRSY
                     VKANEDTVRRTVRVIVEAIHYAKTNREAALKIMQKYLRVQDREALEDAYESFVVKQFP
                     RVPYATPAAVEIIFELAADRNPRAKTADPKGFIDMRFVREIEQSGAIDKLYAQTR"
     gene            2881..3021
                     /locus_tag="VF089_15130"
     CDS             2881..3021
                     /locus_tag="VF089_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175338.1"
                     /translation="MAKKATTKKKSSSKKTSGKKTSAKKTTAKKQSPTPKKKEPIPRY
                     PY"
     gene            complement(3163..3576)
                     /locus_tag="VF089_15135"
     CDS             complement(3163..3576)
                     /locus_tag="VF089_15135"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6175339.1"
                     /translation="MAALTLEMAEKAVRAAQAKARDQGTPMTVTVVDEAGRLVLSARG
                     DGAGFFTPETSRAKAVAAAAFRRPTQEMVRLRDSSPAFWSNISNVLHGEALPSVGGVP
                     VLSSGKVIGAVGCGGGSGEQDHLCAEAGAAAIANG"
BASE COUNT          901 a         1088 c         1022 g          843 t
ORIGIN      
        1 tcgacagctt gatgcacgat ccaatcggct gaatgggcgg caatgccaaa gaggagccag
       61 acacttagat agccggtgat cacctcaatc atcagtcgcg atcgatccgg tctgcgcatg
      121 atcagccggc ggaatatttc aagtagcggc aggctggtcg gcaacatcat cgccgccgtc
      181 atcagtgtcc aaccgagcag atagagtacg acagccagaa agggcgatgc gctgatgccc
      241 agctcgccca gttctgcatg atgcagatag cggccataag gtgagcgatc ccagatccag
      301 ataaccagcc atgctacggc gattaacgag cccatcagcc agaagaaggc tcgacgatca
      361 gcagcggggc ttatgcgcag agcttgcata gagggcggca ggaagcgcaa gcaactctcg
      421 attagtcgga accactacga cgggaatgct cgcgcgagac gaaggcgcca aggtcaaggc
      481 aatgcgtccg caactttgcg gcttcgcgag cagtatcact gtttcctttc gcgaccggca
      541 tcagccttca aaaacaaact ttccctggat cgcgttgtga tccttaaggt caatactctg
      601 acctaattta ggattatcca ttttatattt ggtggccttc gcaacgtatg ccggtgaccc
      661 tgggatggtc gaaaacaccg agtcgtggag cgttgtcaca tcaccgttcg gacctcggaa
      721 gggttccatc tcacaatccg cgacattgcc gatccgcagg gtccccttgc cttcctcaac
      781 cttgaaggta atgggcgagc gctcaactgc gactacttcg ccaatcaatt gaaacaaatc
      841 tgccagcggg ccgccttgct tgccggtgta gacgctgagc agcgcttcct cctgttcctt
      901 agtggccgcc tggtcgatat ggaccaccgc gcgccagttg cccttgagga tgtttcccgg
      961 gacgtgcgcg gtgaaaccca tagtcagccc ggatacgtct acatcatcga ttttccctct
     1021 atcgaaatga tgcgccacca ccgtgtcgca agtcccgtta tccggatcga ccccgatcca
     1081 gcaggggcag agcacctcgc aattgcagac ttcgagaaga cttccctcga gtttgtaagc
     1141 catatgcgtt cccccttcga tttggttagc tcattgatct tatcgctgcg aatttttggt
     1201 gaaataattc ctagacgtcg cttagtaaag tctcgaatga ctgtcaagag ataaatgaag
     1261 cagcggatgt gctacagtct agcgaacctg atgattgagc agttctgatt aaagatatcc
     1321 accctgagtc ttattgaata atttcgcgcc gaaacagcct cggaatcctt gattagtgat
     1381 tttagttatc caccttctct cataaacaat actcctctgg tggcttaagc ggatgtaata
     1441 acctgaggcc tcggcaactg ttagcaagat tgtgcctgct gaacgtaacg ataccgatca
     1501 cttcgacgcg tattgccgtt gcagtgaatc agattgcttt tttccgcggt ggtgtgcgat
     1561 gtcttaatcg ttcaaggagg ggtgttatga aaagcacgtt gatccttagt gctctcgccg
     1621 tctttctgtt gctcccacag cgggcgcccg gacaagagca gctcaaattg atatacagtc
     1681 aattcacgat gacgaactcc gcgacttggt ttgcacgcga ggcggggctt ttcgagcgcc
     1741 acggcttgaa cgcggacctg atctatgtgg actccgcgcc ggcggtgcag gcgctcaccg
     1801 caggcgctgc gcctctggcg acgatgtccg gcgggctggc cgttgggccg tatctcaacg
     1861 gcctggactt ggtcatgctg gcgggctggt gcaacttgaa ctcctaccag cttattactc
     1921 gtcccgagat ccgccgcccc gaagatctgc ggggaaaagt gatcggcatc ggacgctacg
     1981 gcgccgccgc ggattgggcc ttgcgtttga ttcttcgcaa gctcggaatc aatgagcata
     2041 aagatgtaca gatcattcag gcaggcggcg gaggtccggc gactcgcctt ggcgccatgg
     2101 aagccaaaaa gctagacgcc acagtgctcg attcgccgca aaccgttcag gctcgccgct
     2161 tgggcttccg caccttggct gacggggtcg aattgggtat tccctttcta caaggcgggc
     2221 tggtgacccg gcgctcttac gtgaaggcca acgaagacac cgttcgcagg accgttcgag
     2281 ttatcgtcga agcaatccat tatgccaaaa ccaaccgcga agcagcgctg aagatcatgc
     2341 agaaatatct acgcgtccag gaccgcgaag ccctggaaga tgcctacgaa tcttttgtcg
     2401 ttaaacaatt cccgcgggtg ccgtacgcca cgccggccgc ggtcgagatc attttcgaac
     2461 ttgccgccga tcggaacccg cgcgccaaga ccgcagatcc caaaggattc atcgacatgc
     2521 gatttgtccg ggaaatcgag cagagcggcg caatcgataa gctgtacgcc cagacgcgat
     2581 agctcgaccc gtttgagcat gatcaacgaa gctggcagcg cgatcatccg ccacgctccg
     2641 cttccggggt ttgatgcggc gcgagtatcg cgcaatgcgg tgtttcccgg ctcacggcgg
     2701 gctcgggatt actcatcgcg gtatttttat tcagcaagac tggaagacca gcgcaaatcc
     2761 gggcgcgata ttaatttttt ctattctttc cccgatgatt gaagtattat cagacacgtc
     2821 agacagttgc ttagcctaac gacgggttca aaatttcgag acaaaaagga gaaagattga
     2881 atggcgaaga aggcaaccac aaagaagaag tcgtcgagca aaaaaaccag cggtaaaaag
     2941 accagcgcta agaaaacgac cgcgaagaaa cagagcccca caccgaaaaa gaaagagccg
     3001 attccgcgtt atccctatta agctgaaagt gttcggagac gcggatcgga caagccgcgt
     3061 ctcagtgtca gaccgtcgtt tcgcctcgag cagcaggaga ccagaaaggt cgtctgctga
     3121 tcgaggtgca aacgtctgct tacaaccagt ctcaaacgcg cgttaaccat tggcgatcgc
     3181 tgcggccccg gcttcagcac agaggtgatc ttgctcaccg ctgccgccac cgcaaccgac
     3241 ggcgccgatc acttttccac ttgagagaac cggcactccg cctacgctcg gcagagcttc
     3301 gccgtgcagc acgttggaaa tattgctcca gaaagctggg gacgaatctc tgaggcgcac
     3361 catttcttgg gttggtcgtc gaaacgccgc tgctgcaacc gctttggcac gggaagtttc
     3421 aggagtaaaa aagcccgcgc catcaccgcg cgccgacaac accaagcggc cggcttcatc
     3481 gacaacggta accgtcatcg gcgttccttg atcgcgcgct ttcgcctgcg ccgctctgac
     3541 cgccttttct gccatttcca gcgttaatgc agccatatcc ctttactcct ttccaagttc
     3601 ggatcgtgct ttagatacac acgcctgcta aagcccgtga taatgggtag accgagcatg
     3661 ggaacgtttt aaacaatgca tcgagcacat cccgatagcc ttctgtctgc caacaagcgt
     3721 gcattgccag agaattcttc agatgcaagc cgtaatgagt tggagcatcg ttttctcctg
     3781 atcatttttc gcggcagcgc gccagtggcc tcccactact cttctcgaaa acgaaactat
     3841 tcgataggct gata
//
LOCUS       DASUTG010000655         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_991936, whole genome shotgun sequence.
ACCESSION   DASUTG010000655 DASUTG010000000
VERSION     DASUTG010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991936"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF089_15140"
     CDS             <1..656
                     /locus_tag="VF089_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6175340.1"
                     /translation="PTLIHTRSGNAKWLYEISHRNPIWINSKDAEVLDVKTGDLLKIN
                     TEIGYFIDKVWVTEGIKPGVIACSHHLGRWRLQENSGGERWSTALVDLKQTEPGKWLM
                     RHLHGVQPFKSEDPDSERIFWQDGGVHQNLTFPVQPDFISGQHCWHQKVRVTKPAPDD
                     RYGDVFVDTNKSHEVYKKWMLKTRPAPGPENLRRPLWLPRSYKPAPEAYYLNNADRA"
     gene            complement(668..>2521)
                     /gene="recQ"
                     /locus_tag="VF089_15145"
     CDS             complement(668..>2521)
                     /gene="recQ"
                     /locus_tag="VF089_15145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX6175341.1"
                     /translation="STADVLATVRRYWGYSELRPLQEEAIRAGLERRDSLVVMPTGGG
                     KSLCYQVPAELAQRTDIVVSPLISLMKDQVDGLRECGYPAAALYSGMPLHAVQETEEQ
                     VAAGRYRLVFVAPERLLTPRFLQLLTPLEVRAFAIDEAHCISQWGHDFRPEYRQLAEL
                     KTRFPQTSIHAYTATATQRVRADIIEQLRLTKPVVLVGTFDRPNLIYRVVPRIDVQAQ
                     VLEVLRRHPRQAAIVYCLSRNDTEAMAEALKANGLRAACYHAGMEPDARRQTQEAFSS
                     EALDVVAATVAFGMGIDRSDVRCVIHAAMPKSIEHYQQETGRAGRDGLEAECVLLYSA
                     ADVLRWESLIEKSAAGSSPAPESVAASRQLLAQMRRLCTSVHCRHRALSEYFGQDYAK
                     SPCGACDVCFGEVEGLTDATLTAQKILSCVARAGQQFGAEHIIDVLLGARTERVRRWG
                     HDKLTTYSLMKGTDRKSLTNMIYQLLDGGLLERTGDDRPVLKLTDASWSVLRGQRTVQ
                     LLQPKTKIRKSRFEEDTWKNVDTDLFESLRNLRRELAAERGVPAYVLFSDATLREMAR
                     AKPGSAAALLSIRGVGERKLADLGQRFLDHIATYCRANGVAQATPSSSLAK"
BASE COUNT          539 a          731 c          743 g          508 t
ORIGIN      
        1 tgccgacctt gatccatacc cgttccggta acgccaaatg gttgtacgag atttcccatc
       61 gaaacccgat ctggatcaat tccaaagacg cggaagttct tgacgtcaaa accggcgatc
      121 tccttaagat caatacggag attggttatt tcatcgacaa agtctgggtg accgaaggga
      181 tcaaacccgg tgtgatcgca tgctctcatc atctcggtcg ctggcgattg caagaaaatt
      241 ccggcggtga acgctggtcg acagccttag tcgatctgaa acagacagaa ccgggcaaat
      301 ggttgatgcg ccaccttcac ggcgtgcaac cattcaaaag cgaggatccc gactcggaaa
      361 gaatattttg gcaagacggc ggcgtgcacc agaatctgac gtttccagta cagccggact
      421 ttatcagcgg ccagcactgc tggcatcaaa aggtacgcgt gacgaaacct gctcccgatg
      481 acaggtacgg cgacgttttc gtcgacacca acaaatcgca cgaagtctac aaaaaatgga
      541 tgctgaaaac gcgcccggca ccgggaccgg agaatctgcg ccgcccgttg tggttgccgc
      601 gctcctataa gccagccccc gaggcgtatt atttaaacaa cgccgatcga gcgtgaaagt
      661 gactcgatta tttcgctagg ctgctggaag gtgtggcttg ggcaacgcca ttggcgcggc
      721 aataggtcgc aatgtggtcc aggaaacgtt gtccgagatc cgccagcttg cgttcgccga
      781 ctcccctgat gctcaagagc gcagcggctg atcctggttt tgcacgtgcc atctcacgca
      841 gggttgcgtc actgaatagc acatatgccg gaacgccgcg ctcggcggcg agttcgcgcc
      901 gcaagttccg caggctttcg aacagatcag tgtcaacatt cttccatgta tcttcttcaa
      961 agcggctctt acgaatcttg gtttttggct gcagcagttg aactgtccgc tgcccgcgta
     1021 acaccgacca cgacgcgtcg gtgagtttga gaaccggccg atcatcgccg gttcgttcga
     1081 gcaggccccc atcgagaagc tgataaatca tgttggtgag tgacttgcgg tcagtccctt
     1141 tcatcaaaga ataggtggtg agtttgtcat gaccccagcg tcgcacccgt tcggtccggg
     1201 cgcccaggag cacgtcgata atgtgttcgg cgccgaattg ctgtcctgct cgagctacgc
     1261 aggacaaaat cttctgcgcc gtaagtgtcg catcggttaa gccttcaacc tcgccgaaac
     1321 agacgtcaca ggcgccgcat ggcgatttgg cgtaatcctg gccgaagtac tcggacaagg
     1381 cgcggtgccg gcaatgaaca ctcgtgcata atcggcgcat ctgtgcgagg agctggcgcg
     1441 atgcagcgac gctttccggc gccgggctgg atccggcagc gcttttttcg atcagcgatt
     1501 cccagcggag cacgtcggcg gccgagtata agagaacgca ttccgcttcc aagccgtcgc
     1561 gcccggcgcg gccggtctcc tgctgatagt gctcgatgga tttcggcatg gcggcatgta
     1621 tgacgcagcg cacgtcgcta cgatcaatgc ccattccgaa ggcgaccgtc gccgcgacca
     1681 catccaacgc ttctgacgaa aacgcctcct gggtctggcg ccgggcgtcg ggttccatgc
     1741 cggcgtggta acaagccgcg cgcaagccgt tggcctttaa tgcctcggcc atggcttcgg
     1801 tatcgttcct gctgaggcag tagacgatgg ccgcctgccg gggatggcgc cgcagcactt
     1861 cgagcacctg agcctgtaca tcgatgcgag gaacgacgcg gtagatcaga ttgggacgat
     1921 cgaaagtgcc gaccagcacc acgggtttcg tgagacgaag ctgttcgata atgtcggcgc
     1981 gcacgcgctg cgtagcggtg gcggtgtatg cgtgaatgct tgtctgtgga aaacgtgtct
     2041 tgagttcagc cagctggcga tattccggcc ggaagtcgtg accccattgg ctgatacagt
     2101 gggcctcatc gatggcgaag gcgcgaactt cgagaggcgt aaggagctgg agaaatcgcg
     2161 gcgtcaagag ccgctccggc gcgacgaaca ccagccgata tcgtccggcg gcgacctgct
     2221 cttcggtttc ttgaaccgca tgcagaggca tgccgctgta gagggccgcc gccggatagc
     2281 cgcactccct caaaccgtcg acctgatcct tcatcaagga aataagcggc gagaccacaa
     2341 tatcagtgcg ttgggcaagc tcagccggga cttgatagca cagcgacttg ccgcctcccg
     2401 taggcatgac gaccagcgaa tcgcggcgct cgagacctgc ccgtatcgct tcctcctgca
     2461 acggacgcag ctcggagtat ccccagtagc ggcgaactgt agcgaggacg tcagctgtcg
     2521 a
//
LOCUS       DASUTG010000656         7697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_841836, whole genome shotgun sequence.
ACCESSION   DASUTG010000656 DASUTG010000000
VERSION     DASUTG010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841836"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1332)
                     /locus_tag="VF089_15150"
     CDS             complement(112..1332)
                     /locus_tag="VF089_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175342.1"
                     /translation="MSAIAGFFGLSMLAPEKRRSLSLVYAASLALMMGVNFIQPALPA
                     MTEPFGISDSALSLVMTVFTAPAIVLSPIFGVIADMYGRRLLLAGGLILFGVSGTAMA
                     FATSFTWLLVFRAIQGIGFSAVIPLTIVLIGDLLDGDEEIAGQGLKVFLDRVGYMILP
                     PLGGLLAAFAWYWPFTLYIITVPLGFMTFFWMPETKGKITSGTRAYLGEMLKLTRHPR
                     LIVAFSAGFLRFFLDYGFLTYFPLFLVRTHGISTATAGLLYVFFSAGAMITSSQAGRV
                     AAGRDKAQVLFVAFLVSGIALVAVPFMPGVWLIGGVLFFYGIANGVISPMQKSLLTQN
                     APIEMRGGIVSFDRLIQQASKTISTSVVGLLLVTAELPTIFWMLGLLSLVSVALMALL
                     LPSDKSALASPSVS"
     gene            complement(1415..2500)
                     /locus_tag="VF089_15155"
     CDS             complement(1415..2500)
                     /locus_tag="VF089_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX6175343.1"
                     /translation="MRQTGIAQLPLHGGKAPAWLFQRMVRLGREITRLIIENDGPQAF
                     LQRIADPYWLQSLGCVLGFDWHSSGVTTTLCGALKEGLKGREKEFGLIIAGGKGATSR
                     KTPGEIISAGEKYRLDCNPDALVYLSRLTAKVDNNAIQDGFQLYHHSFFFTFDGSWAV
                     VQQGMNERFARRYHWYSARVQTMTLEPHAAICSNLQKERALNLVALDSQPAQSVITDL
                     SHERPEKLIGEIQRLQTLSLPSRHSVSINDIHPKSIDKILLKTYERKATNFEELLSMP
                     GVGAKTLRALTLIADIAYGTPASWKDPARFSFAHGGKDGHPYPVDCKLYDESVEFLRR
                     TLERARIERSDKESALKRLQRLFASQN"
     gene            complement(2509..2754)
                     /locus_tag="VF089_15160"
     CDS             complement(2509..2754)
                     /locus_tag="VF089_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175344.1"
                     /translation="MNQPKKSANPKVTIIASKGANQAINYLLEDRDELEWLVAIGRNK
                     KGDIFFYDTGGDIIQDLGAISYIKERILRDYFGETDE"
     gene            complement(2878..6474)
                     /locus_tag="VF089_15165"
     CDS             complement(2878..6474)
                     /locus_tag="VF089_15165"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX6175345.1"
                     /translation="MAVKARQIFSRARDFTVHPRTRKTVIWAVSLVVAFGVLLGLIAP
                     PLLRGKIAGDLSQKFHREVTIEQIRLNPYAMTATISGFLMKERQSQATAVSLDELHIN
                     LQLQSIFRLSPVVKELRIVKPYINLVRNEDYKYNFQDIIDEFTSGPSGGPTPRFALNN
                     IQLIDGRIEFDDRPEQSKHLISSINIGVPFISSLPSYTDVFVKPSFSANINGAPFHID
                     GASKPFHSSHESTVDLNIDKLQINKYLEYSPVALNFKVPSGELNGKLNGFFKTAQNSP
                     SVFTITGNLALTALAVEKNGGTPFAKIANLDVVIDSIDVFNNKTGLQSIKAHGLELHL
                     ARNRDGSLNTADLVTTSPKPASSEPKKDSKPLGYEVGDILIDSGTLHFSDETLQRPYK
                     TRLDNVRIAVKGLTNEPTKQADIKVSFESDAKERVNHYGKIQLTPFMAEGKLELEGFK
                     PGNLSPYYLSALALEFKEGFLDLTTQYRFDARDNRSDLKFTDLNANLRTVRLDLPGHP
                     EPLWRIPSLVIKDGMVDVPSKTVVISAIQGKSGNGYVQREKDGTLNYARIVKAQSTAP
                     PAKEAANRDETQWNIEAKQIALDGFKVEIDDRAAAAPAKFTLSDLFLRIDNFSNRKNQ
                     PGKTRLRTRINQSGSVRLNGTAGSNPLAAKLAVEAHDIDVLSLQPYFGDQVNFLLTGG
                     RISTKGSFSLAGGTGRAKTNYQGGIELYDFASIEKGNKQDLVKWKNLGLHQFEFNSEP
                     LEVRIGEVNIGDFYSRLIIGADGKVNLQNLTATKQENETAAPAQTAAPSTDTPATERR
                     VTIGKINLHGGNINFSDFLVKPNYSANLTDVQGTISELTPEAPGDIDIQAKLDGSAPV
                     DIDGKINPLGKELFLDIVADAREIEMNPFSPYSGKYVGYGIESGKLSFNVKYKVENRK
                     LTAQNKIVLNQLTFGERVESPQATKLPVLLAVALLKDRNGVIDVDLPISGSLDDPQFS
                     IGGIVLRLVINIITRAVTAPFALLGSAFSGGANGGGEELSYIEFESGRASLSQTAQSK
                     IETLAKAMNNRPALRLQLSGRADPATDLDGLKRVSMERKVKAQKLQELSGKADSPKSV
                     DEVRIESSEYPQYLRQAYRQESFPKPRNAIGLLRDLPVVEMEKLMIQHAAVGEENLRE
                     LANQRAQTVRDALVTTGLVSADRLSIVPAKPLTSEEKAKLKGKPNRVDFSLR"
     gene            complement(6648..7094)
                     /locus_tag="VF089_15170"
     CDS             complement(6648..7094)
                     /locus_tag="VF089_15170"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX6175346.1"
                     /translation="MYYFAYGSNLNWPQMQRRCPSSEFICIARLPDYQFGITRHSRLR
                     NCGTANVIAAPGKEVWGAVYDICDEELLILDSFEDGYRREILPVFGLNVGEAPLPLRA
                     LVYIAELEPDVPLPNTEYKRLILEGARHWQLPSLYLSILEKIEATG"
     gene            complement(7218..>7697)
                     /locus_tag="VF089_15175"
     CDS             complement(7218..>7697)
                     /locus_tag="VF089_15175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175347.1"
                     /translation="VTDIGYSPLIAASFMSTIALTQLGSTLVWGVLADKFDLRKVSCI
                     QFLIQGVGLVIAVASGDIRLVYLGFFLYGTGLAGSFVLREVIWANFFGRASLGKVRGL
                     SLFFSHLFAASGAPFFGFLHDRVGSYNMSFTIFSCALFTSAFLILFAQPPRKASSKE"
BASE COUNT         1798 a         1991 c         2090 g         1818 t
ORIGIN      
        1 gatccaattg gaaaccgaga gccgtgaaga aatggaattc aaaggttttt agtgccactc
       61 ggaacttcac cgggagagga tctcgtgatg gcgccgcatc taacgtttgg ctcaggagac
      121 gcttgggcta gccagcgcgg atttgtctga cggcagcaat agtgccatta gcgctacact
      181 gactagagaa agtaatccaa gcatccaaaa aattgtcggc agctctgcgg tgaccaagag
      241 caaacccacg acggacgtcg aaatcgtttt cgacgcctgc tgaatcaacc ggtcgaaaga
      301 cacgatgccg ccgcgcattt cgatcggtgc attctgcgta agcaggcttt tttgcatggg
      361 cgagatgacg ccgttggcga tgccgtagaa aaatagcacg ccaccgatta accagacgcc
      421 gggcataaac ggcacggcga ccaaagcgat gccactgacc aagaacgcaa caaagagtac
      481 ctgggccttg tccctacccg cggccactcg ccccgcctga ctggaagtga tcatcgcgcc
      541 cgctgaaaaa aagacgtaca acaaccccgc tgttgctgtg gagatgccgt gggtgcgcac
      601 aaggaaaagg ggaaagtaag tcaggaatcc atagtcgagg aagaagcgta agaaacccgc
      661 cgaaaatgcg acgattaggc gcggatggcg cgtcagcttc agcatctcgc ccagataggc
      721 gcgggttccg ctggttatct tgcctttggt ttcgggcatc cagaaaaaag tcatgaatcc
      781 caagggcacg gtgatgatgt aaagagtgaa aggccagtac caggcaaacg cagccagcag
      841 tccgcccagc ggcggcagga tcatgtagcc gacccgatcg agaaatacct tcaaaccttg
      901 gccggcgatc tcctcgtcgc catcgagcaa atcaccaatt aaaacgatcg taagcggaat
      961 caccgcgctg aaaccgattc cctgaatcgc gcgaaacacg agcagccacg taaagctggt
     1021 cgcgaaggcc atggccgtgc cgctgacccc gaagagtata agcccgccgg caagaagcaa
     1081 tcttctgccg tacatatccg cgattacccc aaagatgggc gacagcacga tggccggtgc
     1141 ggtgaacaca gtcatgacca gcgagagagc cgaatcgctg atgccgaagg gctcggtcat
     1201 tgccggcaag gcgggttgaa tgaaattgac gcccatcatt agcgccaggc ttgcggcgta
     1261 aaccaggctt aacgagcgcc gcttctccgg cgccagcata ctcaaaccaa aaaagccggc
     1321 gatggcagac atccgccgca cgctagacca atgcagcgag tgagtcaaac gccgaagtac
     1381 tagtggactt gcgcaagagc tgtcatgaga gaccttaatt ctgcgatgcg aaaagcctct
     1441 gcaatctttt tagcgcgctt tctttatcgc ttctttcaat tcgcgctcgt tccagtgtcc
     1501 ggcgcagaaa ttcaaccgac tcatcgtaca gtttacaatc caccggatag ggatgcccgt
     1561 ccttgccacc gtgcgcaaag ctaaatcttg ccgggtcttt ccaggatgca ggagtgccgt
     1621 atgcaatatc ggcgatgaga gtcaatgcgc gcaaagtctt ggcgccgact ccgggcatgc
     1681 ttaacagctc ttcgaagttc gtggctttac gttcataggt tttcagcaga attttgtcga
     1741 tggatttcgg gtgaatatca ttgatggaaa ctgaatggcg cgaaggcaac gataatgttt
     1801 gcagccgttg gatttcgccg atgagttttt ccggccgctc atgcgagaga tccgtaatca
     1861 ccgattgcgc cggctgactg tccagagcaa cgagattgag cgcccgctct ttttgcagat
     1921 tgctgcagat tgccgcgtgc ggctcgagag tcatggtctg aactcttgcg gagtaccagt
     1981 gatagcgccg ggcaaagcgc tcgttcatgc cttgctgcac caccgcccag gaaccgtcga
     2041 aagtaaaaaa gaagctgtga tgataaagct ggaaaccatc ctggattgcg ttgttatcga
     2101 ctttggccgt gagccggctc agataaacga gcgcgtcggg attgcaatcg agacgatatt
     2161 tctcgcctgc gctgataatc tcgccgggag tttttcggga agtcgcgcct ttgccgccgg
     2221 cgatgattaa gccgaattcc ttctctctgc ctttgaggcc ctctttaagt gcgccgcata
     2281 gggtggtggt cacgccggaa gaatgccagt cgaagccgag aacacaacct aacgactgca
     2341 gccagtaagg atcggctatc cgttggagaa aagcttgggg tccgtcgttt tcaattatga
     2401 ggcgagtaat ctcgcggccc agcctgacca tcctttgaaa tagccatgca ggtgccttgc
     2461 ccccgtggag cgggagttga gcaatcccgg tttgtcgcat agtctacgtt attcatccgt
     2521 ctcgccaaag tagtctcgta gaattcgctc tttgatgtaa gaaattgcgc ccagatcttg
     2581 aatgatgtcg ccgcccgtgt cgtaaaagaa aatatcccct ttcttgttgc ggccgatggc
     2641 cacgagccat tcgagttcgt cacggtcttc caatagataa tttatagctt ggttcgcccc
     2701 tttgctggcg atgatagtta ctttggggtt tgcggatttc ttcggttggt tcatgaaggc
     2761 gcctcctgaa tcgccatgag tttataccag aagattaggc tacacaactt aacgctgaga
     2821 aatgcctttg cacgcttgtg cgcgcaagtt gctcctgtga aataatcagg tacaagatca
     2881 cctcagagaa aaatcgacgc ggttgggttt gccttttagc ttggctttct cttcactggt
     2941 gagcggtttg gcgggaacaa tagacagccg gtctgcgctc accagtccgg ttgtgactaa
     3001 cgcatctcgc accgtctgag cccgctgatt ggcaagctca cgtaaattct cctcgcctac
     3061 ggcggcatgc tggatcatca gtttctccat ttctacaacg ggtaaatcgc gcagcaggcc
     3121 aatggcattg cgcggctttg gaaaactctc ctggcggtat gcttgtctca ggtattgcgg
     3181 atactcgctg ctttcgatac ggacctcgtc aaccgattta ggagagtctg ccttgcctga
     3241 tagctcttgt aatttttgtg ccttcacttt tcgctccatc gaaacccgtt taagcccatc
     3301 caggtccgtg gccggatcgg cgcgaccgct cagttggagg cgtaaggccg gacgattgtt
     3361 catcgccttg gccaacgtct caatcttgct ttgagcagtt tggctgaggc tggcgcgccc
     3421 actctcgaat tcaatgtagc tcagttcttc gccgccaccg tttgcgccgc cactgaaggc
     3481 ggagccgaga agcgcgaacg gcgcagtcac tgctcgagtt atgatgttaa tcaccagccg
     3541 cagcacgatg ccgccgatac tgaactgcgg gtcatcgaga gagccgctga ttggcaggtc
     3601 gacgtcgatg acgccgtttc gatctttgag caatgcgacc gcgagcaaca ccgggagctt
     3661 ggtggcttgc gggctctcga ccctttcgcc gaaagtcaac tgattaagaa ctattttatt
     3721 ctgggcggtt agtttgcggt tctcaacctt gtacttgacg ttgaaagaga gcttgcccga
     3781 ttcgatccca taaccgacgt attttccgga gtaaggtgaa aacggattca tctcgatctc
     3841 gcgggcatcg gcgacgatgt ccaaaaacag ctctttaccg agcgggttga ttttaccatc
     3901 gatgtcgacc ggcgcagatc catccaattt agcttgaatg tcaatgtcgc cgggcgcttc
     3961 cggtgtgagc tctgaaatcg ttccctgcac gtcggttaga tttgccgaat agttgggttt
     4021 gacgaggaag tcgctaaaat tgatgttgcc gccgtgaaga ttgatcttgc cgatggtgac
     4081 gcgcctctcg gtcgccggcg tatcagtgga tggggcggct gtctgcgccg gcgccgcggt
     4141 ctcattttcc tgtttcgtag cggttagatt ttgcagattg accttgccat cggcgccgat
     4201 gatcaggcgc gagtagaaat cgccgatgtt gacctcgccg atgcgcactt cgagcggctc
     4261 tgagttgaat tcgaactggt gcagcccgag attcttccat ttcaccagat cctgtttatt
     4321 gcctttctct atactggcga agtcgtagag ctctattccg ccttggtaat tcgtttttgc
     4381 gcgaccggtg ccgccagcta agctgaagct gccctttgtg ctgattcgcc cgccggttag
     4441 aagaaaattg acctgatcgc cgaaatatgg ctgcagagac agaacatcaa tatcgtgagc
     4501 ttcgacggcg agcttagcgg ccagcggatt actacccgcg gtgccattca agcgcaccga
     4561 tccgctctgg ttgatgcgag tacgcagtct ggtcttgccg ggttgatttt tacggttgga
     4621 aaaattgtcg atgcgaagaa aaagatccga cagggtgaac ttcgccggag cggcggctgc
     4681 gcgatcgtcg atctcgactt taaaaccgtc cagcgcgatc tgtttggctt caatattcca
     4741 ctgcgtctcg tccctattag ccgcctcctt tgcaggcggc gccgttgatt gcgccttgac
     4801 gatacgcgcg tagttcagag tgccgtcttt ttctcgctgg acgtagccgt tgccgctttt
     4861 gccttggatc gcagagatga ccacagtctt tgatgggacg tcaaccatgc cgtcctttat
     4921 gaccaatgac ggaatgcgcc aaagtggttc cgggtgtccg ggcaaatcca ggcgcactgt
     4981 tcttagatta gcattgagat cggtgaactt tagatccgat cgattgtctc ttgcatcgaa
     5041 tcggtattgt gtggtgaggt ctaaaaagcc ttccttaaac tccagcgcca gggcgcttag
     5101 gtagtacggg cttaggttcc ccggcttaaa gccttcgagc tcgagtttcc cttccgccat
     5161 gaacggcgtc agctgaattt taccatagtg gttaacgcgc tctttcgcat ccgattcgaa
     5221 agaaactttg atgtcggcct gcttagtcgg ttcattggtc aatcctttaa cggcaatacg
     5281 gacgttgtcg agtcgggtct tatagggacg ctgcaaagtc tcatcgctga aatgcagggt
     5341 gcccgagtcg ataagaatat cgccaacttc gtagcccaac ggtttgctgt ctttcttcgg
     5401 ctcgcttgaa gccggcttcg gtgaggtcgt gaccagatcg gcggtattca aagatccgtc
     5461 gcggttgcgg gctaagtgca actccagtcc gtgagctttg atggattgta agccggtctt
     5521 attgttgaat acgtcgatgg agtcgatcac gacatcgagg ttggcaatct tggcgaacgg
     5581 cgtgccgccg tttttctcga cggcgagcgc cgtgagcgcc agattcccgg tgatcgtgaa
     5641 taccgatggg ctgttctgcg cggtcttgaa aaatccgttg agcttgccat tcagttcgcc
     5701 ggaaggaact ttgaagttca gagctaccgg cgagtattcg agatatttgt tgatctgcag
     5761 tttgtcgata tttagatcga cggtgctttc gtgcgagctg tggaatggct tggaggctcc
     5821 gtcgatgtgg aagggagccc cattgatatt cgccgaaaag gacggcttga cgaaaacatc
     5881 agtataggac ggcaggcttg aaataaacgg cacgccgata ttgatcgatg agatcaagtg
     5941 cttgctttgt tccggccggt cgtcaaattc aatccggccg tcgatcaatt gaatattgtt
     6001 taaggcaaac cgcggcgtcg gaccgccgct tggtccggaa gtgaactcat cgatgatgtc
     6061 ttgaaagttg tacttgtaat cctcgttgcg caccagattg atgtagggct ttacaatgcg
     6121 aagctctttg acgaccgggc tcaaacggaa aatagactgg agttgcaggt tgatgtgcag
     6181 ctcgtctaaa gacaccgccg tggcttgact ctgacgctct ttcatcaaaa aaccgctgat
     6241 ggttgcggtc atggcatatg gattcaagcg gatctgctcg atggtgactt cgcgatgaaa
     6301 tttttgagat aggtcaccgg ctattttgcc gcgcagcaag ggtggagcaa tcagtcccag
     6361 cagcacgcca aaggcgacaa caagcgagac cgcccaaatg actgttttgc gagtacgcgg
     6421 gtgaacagtg aaatctcgcg cacgactgaa gatctgcctt gctttaactg ccattggtaa
     6481 aaattggttt taaacttgcg cgatattact ctagaggata gggcttgtcc acgaagatac
     6541 tgccgagctg ctatcgtaaa cgagtcctat cgggaggttt tcgatgagac gccaaaacag
     6601 tttgcgggca gaattctctt tggccggaac tctgatcgtt gacccaacta tcctgttgcc
     6661 tctatcttct cgaggatcga aaggtagagg ctggggagct gccaatgtct cgctccttct
     6721 agaatcaatc gcttgtactc cgtattgggc aaggggacat caggctccag ctcggcaata
     6781 taaaccagcg cccgcaacgg caacggcgcc tctccgacgt taaggccaaa aaccggcaaa
     6841 atctcccgcc gatagccgtc ttcaaagcta tccaatatga gcaactcctc atcacagata
     6901 tcatatacgg cgccccaaac ttccttgcct ggggctgcga tcacatttgc cgtgccgcag
     6961 ttgcgcaacc gcgaatggcg cgtgatgccg aattgataat ccggcaacct ggcgatgcaa
     7021 atgaattccg atgacgggca acgacgttgc atttgtggcc agttcaggtt ggagccgtag
     7081 gcaaaataat acaacgggct tatcctccta ctcgtatctg cgataacaaa caattatgtg
     7141 actaggaaaa aagttgtcaa cagcggccgc cggaattaag tgattaacat aaggcctaag
     7201 ccagagctaa ttacatttta ttccttgctt gatgctttcc tcggcggttg ggcaaacaag
     7261 atcaaaaacg cggatgtgaa tagagcgcaa gagaagattg tgaacgacat attgtaactg
     7321 cctacgcgat cgtgaagaaa gccgaaaaaa ggcgcgccgc tggcggcgaa tagatgggaa
     7381 aagaaaagcg acagtccgcg cactttcccc agagacgctc gaccgaagaa attagcccag
     7441 ataacttcac gcagaacaaa gcttccggcc aatccggtgc cgtagagaaa aaaccccaag
     7501 taaacaagac gaatatcacc gctggcaacc gcaatcacca gcccaactcc ctggatcaag
     7561 aattgaatgc aggatacctt cctaagatcg aatttatcgg cgagtacacc ccagactagt
     7621 gtggagccga gttgagtcag cgctatagtg ctcataaaag aagccgcaat cagcggcgag
     7681 tagccgatat ccgtgac
//
LOCUS       DASUTG010000657         3945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2028014, whole genome shotgun sequence.
ACCESSION   DASUTG010000657 DASUTG010000000
VERSION     DASUTG010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028014"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1183
                     /locus_tag="VF089_15180"
     CDS             29..1183
                     /locus_tag="VF089_15180"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX6175348.1"
                     /translation="MKFSTDRILTTHAGALPQPDDLKQLHTAKISGQPVNEAAFAQRV
                     HQAVADVVKKQLACGLDIINDGEVGKSNFSRYARERLSGFIEREAKPEDHVSSIFARD
                     RVEFGDYFRRRMGGIRGENVKRVFCNGPLKYVGHASLRAEIEDFKAGLQGQSYGEAFL
                     PAIAPGTIEHWMKNDYYPDDESYLIAIADAMHEEYKAIVDAGFLLQIDDPDLPDGWQF
                     MSQMTVPEYRRYAELRVDALNHGLRDIPPEKVRFHTCWGSYHGPHKFDIPLRDIVDII
                     LKVNANTYSIEASNPRHEHEWRVWQEVKFPQGKVIVPGVVGHATDIVEHPEVIAERLL
                     RYARIVGRENVMAGTDCGLGPRVGDPQIAWAKFEAMAEGARIASKQLWGK"
     gene            1338..2378
                     /locus_tag="VF089_15185"
     CDS             1338..2378
                     /locus_tag="VF089_15185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX6175349.1"
                     /translation="MRFEGRLPWSVAALFVLALALSPWSAQAQQLKSVAIGSNPPGSL
                     FYALASGLSKIISESSPIQAQVQPHAGTSTFLPLLDSGELDLGVVNAVDMALAYQGPE
                     RLQIGGKNPFPHVPNTRLIMRGSPLRSSLIVRKDSPIKNIAEVKGKRVTGEYPAQLAV
                     WYNVFGALSNGGLTWKDVKVVPVPGVNEGVDALVQGRAEVTTHAIGSAKVREADAAIG
                     IRYIPLDCSAQGLERIKKAVPGYYLSTVKAGASSGIVEDTCAYTYDIYLVGHKALPDA
                     AVTTMLKGIWENIAKLPPLHPGFAEWTRERAVDPEVTIPYHPAAVQFYKEQKSWPAKM
                     DEVQKKLLSLNP"
     gene            2410..>3945
                     /locus_tag="VF089_15190"
     CDS             2410..>3945
                     /locus_tag="VF089_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX6175350.1"
                     /translation="MADDLAPNAKFRTLNGAAAALVRLFLCTLTILGALWSLEIHDTL
                     EWTFFKEQYLGLFFAIAMAGIFLCVKSRPAESGLVIPWYDWILAGASLLAGGYVTVMY
                     PTIAYRLGVLSPERWLLGGMAILLILEATRRVVGWALVWVAVPIILYAKFAYLLPGML
                     NSRGSSWQRIASYLYLDSSGIFGLPFDVAAGIIVAFIFFGQALYAVGGDKFLTDIALV
                     LMGRYRGGTAKVAVVSSTLFGTVSGSAVANVVVDGAITIPMMKKSGYPPHVAAAIEAV
                     ASNGGQIMPPVMGVAAFLMAEFLNISYAEVALAAAIPACLYYIALFTQIDLEAARLGL
                     VGMRDDDIPKLGHVMKRGSVFLVPLLVLVYALMVANWEPGKAGMAAAIVTFVVGALGK
                     DTRPSPKGILTSIEETGKILLDIVVVTALAGLVIGAFQLSGLTFKFALLLVSTAGGNI
                     PLLLLLTAVVSIILGMSMPTTVVYIMLAVLVGPALVQVGIPPLAAHLFFFYFGMLSLI
                     TPPD"
BASE COUNT          810 a         1123 c         1105 g          907 t
ORIGIN      
        1 acggcttgaa cgatcattcg gaggaataat gaaattcagc actgaccgaa tactgaccac
       61 tcacgccgga gccttgcccc aacccgatga tctaaaacag ttgcacacag caaagatcag
      121 cggccagccc gtcaatgagg cggcttttgc gcagcgggtg caccaagcgg ttgcggacgt
      181 ggttaaaaaa cagttggcgt gcggcctcga tatcatcaac gatggcgaag tcggcaagtc
      241 caacttctcg cgctacgccc gggagcgcct gagcggcttc atcgagcgcg aagcaaaacc
      301 ggaagaccac gtctcttcga tctttgcgcg cgacagggtc gagttcggtg actatttccg
      361 caggcgcatg ggcggcatcc gcggcgaaaa cgtcaagaga gtcttctgca acgggccgct
      421 caaatacgtc ggtcatgcct cgcttcgagc tgagatcgaa gattttaaag ccgggctgca
      481 gggacagtcc tacggagagg ctttcctgcc ggcgatcgcg ccgggcacca tcgagcactg
      541 gatgaagaac gactattatc ccgatgacga gtcatacctg atcgccatcg ctgacgcgat
      601 gcacgaggag tacaaggcaa tcgtcgatgc cggctttttg ctgcaaatcg atgatccgga
      661 tttgcccgac ggctggcagt tcatgtcgca aatgacagtg ccggaatacc gccgctatgc
      721 ggaactgcgc gtcgatgcgc tcaatcacgg cttgagagac atcccgccgg agaaggttcg
      781 ctttcatacc tgctggggca gttatcatgg gccgcacaaa ttcgacattc cgctgcgtga
      841 catcgtcgac atcatcctta aagtcaacgc caatacctat tcgatcgagg cctccaaccc
      901 gcgccacgag catgagtggc gggtgtggca agaggtcaaa tttccccagg gcaaagtcat
      961 cgtccccggc gtcgttggac acgccaccga catcgtcgaa cacccggagg tcatcgccga
     1021 gcgtttgctg cgctatgcca ggattgttgg ccgggaaaac gtcatggccg gcaccgactg
     1081 cggtctcgga ccgcgggtcg gtgatccgca aattgcctgg gcgaaatttg aagcgatggc
     1141 cgaaggcgcg cgcattgcga gcaaacagtt gtggggaaaa tgatgcgtgc ggcagcaaac
     1201 tctgttgaat gcataccaag acaccgcgca gacgctcgta agatcaattg atgaccggaa
     1261 caccattgcg acttgcctcg atttgacacc acaagaacgt ttggtttaga ttgcgcgcca
     1321 accgaaggag gatcgttatg cgcttcgaag gtcgtctacc ctggtctgtg gcggcactgt
     1381 ttgttcttgc tttagccctc tcaccttggt ccgctcaggc gcaacagctc aaatctgtcg
     1441 ctatcggatc caacccgccg ggttccttgt tttatgcatt ggccagcggt ctatccaaaa
     1501 tcatcagcga atcatcgccg attcaagcgc aagtgcagcc ccatgcaggc accagcacct
     1561 ttttgccgct actcgacagc ggagagctcg atctaggcgt tgtcaatgcg gtcgatatgg
     1621 cccttgccta tcaagggccc gagcggttac agattggcgg aaaaaatccg tttccgcatg
     1681 ttcccaacac tcgcctgatc atgcgcgggt cgccgctacg cagcagtctc atcgtcagaa
     1741 aggactcgcc gattaaaaat atcgcggagg tcaaaggcaa acgagtcacc ggcgaatatc
     1801 cggctcaact tgccgtctgg tacaacgttt ttggcgctct ctccaacggc ggtctcacct
     1861 ggaaagacgt caaagtagta cccgttccgg gagtgaacga aggagtcgat gcgttggtgc
     1921 agggtagggc cgaagtcacc acccatgcaa tcggctcagc aaaggtccgt gaagctgatg
     1981 ccgccatcgg aatccgttac ataccgctgg actgctcggc tcaaggcctg gagcgcatca
     2041 agaaagctgt gccgggttac tatctttcga cggtcaaggc cggggcttcg agcggcatcg
     2101 tcgaagatac ctgcgcctat acgtatgaca tctacctcgt cggccacaaa gcgcttcccg
     2161 atgctgccgt tacgactatg ctcaaaggta tttgggaaaa catcgccaag ctgccgccgc
     2221 tacatcccgg cttcgctgag tggacccgag aacgagcggt ggacccggaa gtgacgattc
     2281 cctatcaccc ggccgccgtg cagttttaca aggagcaaaa aagttggccc gctaaaatgg
     2341 acgaggtaca aaagaagctg ctttcgctaa acccataacc atcctgccag ttccctcagg
     2401 agttaagccg tggctgacga tcttgctccg aacgccaagt tccggacgtt aaatggagcc
     2461 gcggcggcat tggtccgctt gtttctgtgc acgctcacaa tcttgggcgc gctctggtct
     2521 ttagagattc acgatacgct ggagtggaca tttttcaagg aacagtatct tgggcttttc
     2581 ttcgcaatcg ccatggccgg tatttttctg tgtgttaaat cccggcccgc cgaaagcggc
     2641 ttagtcatcc catggtacga ctggatactc gccggcgcca gcttgctggc aggcggttac
     2701 gtgaccgtca tgtatccgac cattgcctat cggttgggag tgctttcacc cgaacgttgg
     2761 ctcctcggcg gcatggcgat cctgctcatt ctggaagcga ctcgccgcgt ggtcggttgg
     2821 gcgcttgtgt gggttgctgt accgatcatt ctctacgcca agtttgccta tctcctaccc
     2881 ggtatgttga attcaagagg ttcctcatgg cagcgcatcg ccagctatct ttacctcgat
     2941 tccagcggca tcttcggtct gccttttgat gtcgcggcgg gaatcattgt cgctttcatt
     3001 tttttcggcc aggcgctata tgccgttggc ggcgataagt ttctcaccga tatcgctttg
     3061 gtgctgatgg gccgctaccg gggcggaacc gctaaagtcg cggtcgtctc ctcgactctc
     3121 ttcggcacag tgtcgggcag cgccgtggcc aatgtcgtcg tcgatggcgc catcactatt
     3181 cccatgatga aaaagagcgg ctatccgccg catgtggccg cggccatcga ggcggtcgca
     3241 tccaatggcg gccagattat gccgccggtc atgggtgtag cggcgttttt gatggccgag
     3301 ttcctgaaca tttcttacgc cgaagtagcc cttgctgcgg ccattcccgc ttgcttgtac
     3361 tacatcgcat tattcacaca gatcgatctc gaggcggcgc gcctcggtct ggtcggcatg
     3421 cgggatgacg acattcctaa gcttgggcat gtcatgaagc gcggatcggt atttctggtt
     3481 ccgctattgg tattagttta tgcgctcatg gtcgccaact gggaaccagg taaagcgggg
     3541 atggctgcgg ctatcgtaac atttgttgtg ggcgcgctgg gaaaggatac ccggcccagt
     3601 cccaaaggca ttttaaccag catcgaggag accggcaaaa ttctcctcga tatagtggtc
     3661 gtcaccgcgc tcgcgggact cgttatcggc gcctttcagc tttccggcct cacgtttaag
     3721 ttcgcgttgt tactggtcag tacggcgggc ggcaatatcc cgcttctgct gctcttaacc
     3781 gctgtcgttt caatcatcct cggtatgtct atgccgacta ccgtcgtcta catcatgctg
     3841 gcagttttgg ttgggcccgc gctggttcag gtcggcattc ctccgctggc ggctcatctt
     3901 tttttcttct acttcggcat gttgtccttg atcacgccac cggat
//
LOCUS       DASUTG010000658         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2028088, whole genome shotgun sequence.
ACCESSION   DASUTG010000658 DASUTG010000000
VERSION     DASUTG010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028088"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..486)
                     /locus_tag="VF089_15195"
     CDS             complement(214..486)
                     /locus_tag="VF089_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175351.1"
                     /translation="MAGWIFGIAVFAAGVVNTFWGNDPGFGVFILLLSLVYFPPTSNI
                     LGAISKRLLGFPIPKVARIVLGIFIIWATLGVGELFDKINLMMMDL"
     gene            1280..2527
                     /locus_tag="VF089_15200"
     CDS             1280..2527
                     /locus_tag="VF089_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6175352.1"
                     /translation="MLIAEDELGRMHKRFPYPRFSVAEYERRYSNIRAMMKDKNLDCL
                     LIIGGSAAYGRLWFNVRYVTNMMGKAEMANYCFFPKDGAPCLVVRPGHSLAGGMLART
                     AVREVIVGDPNVLAGIVNTIQERGYEKGRIGIVEYDPFTSIPKNHWEFFTARLPQAEF
                     IFVTREFIALRLLKSEEEIEALERSAELGDVAILAMAERVKPGMTEGELFGIAHEAAL
                     KAGGEMGMIQLGSAPMEDPDVNDQRPRPVGRVIGPRDFINNEIGIFYNGYESQTGKPI
                     VTGTPTKWFEEMFEIALEAYKRIAPTLQPGKSSADSLEAAKFVLDTGYEYYGGFLQGM
                     LGANPRHEPQIGFDRVQSGEDRFLFDADGNFTYKPGLMFTLQVHLIDESHTRSLFLGD
                     SYVITEGGAKCLNKVPPQMFRIK"
     gene            2548..>3424
                     /locus_tag="VF089_15205"
     CDS             2548..>3424
                     /locus_tag="VF089_15205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175353.1"
                     /translation="MKHGLKAFDSDMHVYDHPDLYMKYMNPKWGDRIPVAEGWTKHGR
                     PQFKIGSGAKLRPREALLDHGEKQVEQRYGFAVARGYDAVSQVQAMDMEGLDVAVLFR
                     TSPLHCDETFEPEYANDLCKAWNSWIADFCRVSPKRLKASALITLHDVDLAIEEVRRA
                     VNELGVSGLSLCPEPINGKFIHDRHFDPLWAEIEKLGVALCFHPPARPKQDQVANKFF
                     GHPNGDIVALALRNPVELILAVSEFCAGGVLEKFPKLRVAFLEGNCAWLPWLLYRLDE
                     RAKLHGPLADVPLSRK"
BASE COUNT          858 a          839 c          938 g          789 t
ORIGIN      
        1 catctggccg cgattggtga agtcatatcc gcaattcgat gcgtatcagc atcgcacgag
       61 ccgtggaatc ccgctgttga ggttaaccgc gaaaacctag gccgtgaact gacaagacgg
      121 tgaactttaa ccgcttctcg attcctctcg gatcccgagg agcagtataa aggaatattt
      181 cgaaccaaaa gttctattga tatagcttga atattataaa tccatcatca tgaggtttat
      241 cttatcgaac aattcgccca cgccgagcgt tgcccaaata ataaagatgc ccaagacgat
      301 tctggctact tttggaatcg ggaaaccgag caatcgctta gagattgcac caaggatatt
      361 actcgtcgga ggaaagtaaa ccaaagaaag aagaagaata aaaactccga agccggggtc
      421 gttcccccaa aacgtattca caacgccggc tgcaaaaaca gctatgccaa atatccaacc
      481 ggccatattt aatatcatca ttctacttca gttacattta tttgataatc aacgaaacgc
      541 atatagacag agcttattcc ccgcaatcca taagtttatt cgctgaaaga gccggacgtg
      601 ttgaatctcg gcttttttgt caacgatcga gttaaaatca gcaacgcacg aaccgcgaaa
      661 tcccgctgtt gagattaacg cgcaagatca ggccggaata gaagaagacc tggctggtcg
      721 tttcaacaac gtcggtgcat atgttccgat cttcaatctt ttgcatttcc ccacttgtac
      781 gacagttttc gctttgacag aaaacctgca ttttactaga aaaaggaatc ctccatgacc
      841 gctacggaaa aatacattcg acggctcgaa acgatgagca atgggcaacg gctactaagg
      901 tgaatatctg ctcagcgtta gttccggact gctcgtgtcg actgcgaagg aagttgccca
      961 cacaatgggg ttggcgagtc taccgtcgtg cgatttgcga aggaacttgg ctatcgcggc
     1021 ttcctgatct aaagcggaat ccccggaaag aactcggtgc acggctgcgg gccgcgccga
     1081 gaatggatca aaccatcgca ggcatcggca aaacggaaac gtcctcacaa acctgaaaaa
     1141 aaaggatatt cagctgctgc gctcgacctt agagagcatg ctggcgattg cgggagtaaa
     1201 aaaaagcgat tcgcctcaaa gctttgaaga atctagatca gttgttggaa acttcctgtg
     1261 tcagtaaagg aggcgtggga tgcttatcgc tgaagacgaa ttgggacgca tgcacaagcg
     1321 gtttccctat ccgagattct ccgttgcgga atacgaaaga cgctatagca atattcgggc
     1381 gatgatgaag gataaaaatc ttgattgcct gctcatcatc ggcgggtctg ccgcctatgg
     1441 ccgcctctgg tttaacgttc gctatgtcac caacatgatg ggtaaagcgg agatggccaa
     1501 ttactgtttc tttcctaagg atggcgctcc ctgcctggtc gtgcgtccag gacattcgct
     1561 tgccggcggc atgctggctc gaaccgctgt gcgcgaggtc atcgttggtg acccgaacgt
     1621 tttggcgggg atcgttaata ccatccagga gaggggttac gaaaagggtc gaatcggaat
     1681 cgtcgaatac gatccgttta cctcgatccc taaaaatcac tgggagttct tcactgcccg
     1741 gctgccgcaa gccgaattca ttttcgtgac cagggaattt atcgcgcttc gtttgctcaa
     1801 aagcgaggag gagattgagg cgctggaacg ctccgccgag cttggcgatg ttgcgatttt
     1861 agcgatggca gaaagggtta agcccgggat gaccgaaggc gaactgtttg gcatcgcgca
     1921 cgaagcggcc ctgaaggctg ggggcgagat gggcatgatt cagcttggct cggcgccgat
     1981 ggaggatcct gatgttaacg atcagcgacc gcgcccggtg ggccgggtga tcgggccgcg
     2041 cgatttcatc aataatgaaa tcggcatttt ctataacggt tacgagtccc agactggcaa
     2101 accgatcgtg accggaacgc cgaccaaatg gttcgaagag atgtttgaga tcgctttaga
     2161 agcctacaaa cggatagcgc caactctaca accgggaaag tccagcgctg actcgttgga
     2221 agcggcaaag ttcgttctcg acacaggtta cgaatattat ggcggctttc ttcaaggcat
     2281 gctgggcgcc aacccgcgcc acgagccgca gatcggcttt gaccgggtgc agagtggcga
     2341 ggaccgtttt cttttcgatg ccgacggtaa cttcacctac aagccgggtc tcatgtttac
     2401 cttgcaggtt catctgatcg acgaatctca tactcgcagc ttatttttgg gggacagtta
     2461 tgtgattacg gaaggcggcg caaaatgtct taacaaggtt ccgccgcaga tgtttagaat
     2521 taagtaagct aaggaaaggg agatagaatg aaacacggtt taaaggcgtt tgatagcgat
     2581 atgcatgttt acgatcaccc cgatctctac atgaagtaca tgaaccccaa atggggtgac
     2641 cgcattcctg ttgccgaggg ctggacgaaa catggccggc cgcagttcaa gatcggcagt
     2701 ggcgctaagc ttcgaccccg ggaggcgttg ctagatcatg gtgagaagca agtagagcaa
     2761 cgatatgggt ttgccgtggc ccgtggatac gacgcagtat cgcaggtcca agcgatggac
     2821 atggaaggac tcgacgtcgc ggtgttgttt cgtacttcgc cgctccattg cgatgagacc
     2881 ttcgagccgg aatatgccaa tgatctatgc aaggcgtgga acagctggat tgccgacttt
     2941 tgccgggtca gccccaagcg tttgaaggcg tcggcgttga tcacgctgca cgatgtcgat
     3001 ttggcaatag aggaggtgcg gcgagctgtc aacgagcttg gcgtgtccgg cctttcgctt
     3061 tgtcccgagc ccatcaacgg gaagttcatt cacgaccggc acttcgatcc tctgtgggcg
     3121 gagatcgaaa agctcggcgt tgcgctttgc tttcatccgc cggcaagacc aaagcaggac
     3181 caagtcgcca ataaattttt cggtcatcca aacggcgaca tcgtcgccct ggcattgcgc
     3241 aatccagtcg aattgatcct ggcggtttcc gagttttgcg ctgggggcgt actcgagaag
     3301 tttcccaaac tgcgggtggc gttcctggaa ggtaactgcg cctggttgcc atggctgctc
     3361 taccggctcg atgagcgggc aaagttgcat ggtccgctgg ccgatgtgcc gttatcgagg
     3421 aagc
//
LOCUS       DASUTG010000659         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_250237, whole genome shotgun sequence.
ACCESSION   DASUTG010000659 DASUTG010000000
VERSION     DASUTG010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250237"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VF089_15210"
     CDS             <1..1211
                     /locus_tag="VF089_15210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175354.1"
                     /translation="PQATLLALDQKGTLFVRYSEQKQWPSTSTLEPSLVKTIIEQKEG
                     LTEAEGSDGVRRLFGFTTLHRLSNTGALFVSVGIPTEVAFANSKRIFYASLVVFMVAS
                     LLAAAGTWLGAHLLIGRRLEKLITAARALGDSEYNQAPRSAEELAKTAGQFGEVLNEM
                     RVALHKASGRQADFAAMIAHDFRNALNTILCAASLLPEPGQTKHNEQVFIDMIRRGCD
                     ELTQMLNEFLDFSKYRAGHLQLEKEQFDLYYFFKQLENQYRWRTQQKKIAFHVEVDPG
                     VGSITADRKKLHQLLDNLLSNALKFTNEQGEIRIGARQVNGATELWVKDTGIGIAPTE
                     CNTLFSLYSQTASARSSTEKGTGLGLLICKMIAEAHGGQISVESEPGKGTTFHVWLPR
                     TATSPLRIDT"
     gene            complement(1383..2021)
                     /locus_tag="VF089_15215"
     CDS             complement(1383..2021)
                     /locus_tag="VF089_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6175355.1"
                     /translation="MSAKETSIAVIAAFGLFTISLLGPNRGFAHCDGLDGPVVRAAQR
                     ALETKNVNLVLIWVAKDDEPEVKEAFQKTIAVRKLDPQAKELADLYFFEKLVRIHRAG
                     EGEPYTGLKAAGRDLGPAIPAADRALESRKLEPLLKLLTQEIKSGIGHKFEDVLAKKD
                     YQLDNVQAGREYVEAYVRFLEHVERIHESAKNPGRGHLEEFGKPVLHKEEKR"
     gene            complement(2180..>2439)
                     /locus_tag="VF089_15220"
     CDS             complement(2180..>2439)
                     /locus_tag="VF089_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175356.1"
                     /translation="KKFDKPGVSRVLCNAHPNMEAFIVVKDHPFFSTTDSRGNYEIKD
                     APLGKLRVEIWYPNLDVRSDSVEIVRDGEVFALSVELKKRK"
BASE COUNT          569 a          656 c          609 g          605 t
ORIGIN      
        1 cgccccaggc gacccttctg gcgctcgatc agaaagggac tctattcgtt cggtattctg
       61 aacagaagca atggccctct acgtcaaccc tcgaaccttc cctggtgaaa acaattatag
      121 agcagaagga aggattgacc gaggcggaag gttcggacgg agtgcggcgt ctctttggtt
      181 tcacgactct tcatcgcttg tcgaacactg gcgctctatt tgtgagcgtt ggaataccta
      241 ctgaagttgc tttcgccaat tcaaagcgaa tcttttacgc tagcttagtg gttttcatgg
      301 tcgcttcgct tctggctgcc gcaggcacct ggttaggcgc tcaccttttg attgggcgaa
      361 gattagagaa attgatcact gccgctcgcg cacttggtga cagtgaatat aatcaagctc
      421 cgcggtcggc ggaggagctt gcgaaaacag ccggccaatt cggtgaggtc ctcaacgaaa
      481 tgcgggtcgc gcttcacaaa gcaagcggcc ggcaagcgga ctttgcggcg atgatcgcgc
      541 acgattttcg caatgctctc aacaccattt tatgcgcagc ctcgctgctg cccgagccgg
      601 gccagaccaa acacaatgaa caagttttca tcgatatgat ccgccgcggc tgtgatgaat
      661 tgacgcaaat gctaaacgag ttcttggatt tctctaagta tcgagcaggg cacctgcaac
      721 tcgagaaaga acaattcgac ttgtattact tcttcaagca gctggagaat caataccgtt
      781 ggcgaactca gcagaagaag atagcttttc atgttgaagt ggaccctggg gttgggtcga
      841 tcaccgccga tcgtaaaaaa ctgcaccagc ttttggataa tctactcagc aacgctttga
      901 agttcaccaa tgagcaaggt gaaattcgga ttggagcacg acaagtaaac ggggcgacgg
      961 agttgtgggt aaaagatacc ggtatcggta ttgctccgac ggaatgcaac acgctgttct
     1021 cgttatacag tcaaacggct agtgcccggt cttcaactga aaaaggcaca ggactcggtt
     1081 tgctcatctg caaaatgatt gcggaggcgc acggcgggca aatatcggtt gaaagcgagc
     1141 ctggtaaggg aacgaccttc catgtgtggc ttccgcgcac ggccacttcg cctcttagaa
     1201 tcgatacata gaaggcggtt gtcggcccat gaggtccgtt gccgggtgtt tgaactacca
     1261 ccaaccctaa aggacagcta aaattgcgta cggctaaaag cctgtaacaa cgccctcagc
     1321 ctgagtttta cataccaacc ttcaaccgag ttggaattgt gaatgtcatg tgaccgccct
     1381 gtttatcgct tttcttcttt atgcaatacc ggtttgccga attcctctaa gtgaccgcgt
     1441 ccagggttct tcgcggactc atgaatccgc tcgacatgtt cgaggaaacg cacgtaagct
     1501 tcgacgtact ctcggcccgc ctggacgttg tctagttggt agtctttctt cgccagaaca
     1561 tcttcaaact tgtggccgat gccacttttt atctcttggg tcaacagctt gagcagcggc
     1621 tcaagcttac ggctttccaa agcccgatct gccgccggaa ttgcaggccc gagatcgcgc
     1681 ccggctgctt taagaccggt gtaaggttcg ccttctccgg cccgatgtat gcgaaccaac
     1741 ttttcgaaga aatataggtc ggcgagctct ttcgcttgtg gatctaactt gcgcactgca
     1801 attgtcttct gaaaagcctc ctttacctct ggctcgtcat ctttcgccac ccagataaga
     1861 accaggttca cgttttttgt ttccagcgcc cgctgggctg ctcggaccac aggaccgtcc
     1921 agcccgtcgc aatgcgcaaa gcccctattc ggtccaagaa gacttatggt aaataggcca
     1981 aaagcagcaa tgactgcgat cgaagtctct ttagcgctca tttgtatcct cccttccttc
     2041 gttcccttcg atgaaacgta tcgaaacgac ctgcctgatt ggaaggttca atcagccgcc
     2101 gttctcattg aggcagcaca aacggctaca cgtttactct caggcgcgca gatttcctcg
     2161 ctcatggtta atgctcagat cacttccgtt tcttaagctc gacactcagg gcaaacacct
     2221 cgccatcgcg gacgatttcg acgctgtcgc tcctgacatc gaggtttgga taccaaatct
     2281 ccacgcgcag cttacccaag ggtgcgtctt tgatctcgta gttgccccgg ctgtccgtgg
     2341 tcgaaaaaaa cgggtgatct ttcacgacga tgaaagcttc catgttcgga tgtgcattgc
     2401 acagaactcg agaaacgccc ggcttatcaa acttcttgc
//
LOCUS       DASUTG010000660         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1436376, whole genome shotgun sequence.
ACCESSION   DASUTG010000660 DASUTG010000000
VERSION     DASUTG010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436376"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2508)
                     /locus_tag="VF089_15225"
     CDS             complement(<1..2508)
                     /locus_tag="VF089_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6175357.1"
                     /translation="MAIDPICGMKVEPARAAGEHEHNGQIYYFCSQHCLAKFKENPAQ
                     YVKTAASSQVADAEQGSGQRRSVKDPVCDMTVDPSSAAGKQEYKGKIYYFCSHHCAQK
                     FKEDPDKWLGEQRGGHVVPTPAREHTASRNHVHVRSDEAAYVCPMDAEVRESKPGACP
                     KCGMALEPETLAASSTRTEYVCPMHPEIVRDEPGSCPICGMALEPRTVTLEDAPNPEL
                     VGMTRRFWISAALSLPLFIIAMSDMISGQPLQHILSSRLLTWIQFILATPVVLWGGWP
                     FFERGWQSIVNRSLNMFTLIAIGVGASYLYSVVATLFPGIFPESFYGHSGTVAVYFEA
                     AAIITTLVLLGQVLELRARSQTSSAIKALLGLAPKTARVMYEDGREEDVLLDLIQVGD
                     RLRVRPGEKVPVDGVVIEGASSIDESMITGEPIPVEKAKGSKVTGATVNGTGSIIMEA
                     QRVGSETLLAQIVRMVSEAQRTRAPIQRLADIVSAYFVVAVIVVAALTSIVWALFGPE
                     PRMAYAIVNAVSVLIIACPCALGLATPMSIMVGVGRGATEGVLIKNAEALETLEKVNT
                     LVVDKTGTLTEGKPQLSEVVSVAPTDRKEILRLAASIERASEHPLAAAIVAAAEREGL
                     RLANAEEFRSLTGRGVIGKVDGRSVALGNIKLLEELSIEPGTLLARAEELRREGHTVM
                     FVGVDGRAAGLVAVSDPIKQSTAEAIQILHQEGVRMVMLTGDSRTTAEAVARRLGIDQ
                     VEAEVLPEQKAEVIKRLQDEGRIVGMAGDGVNDAPALAQAQVGIDMGTGTDVAIESAH
                     VTLVKGDLRGIAKARRLSRATMRNIRQNLFFAFVYNVM"
     gene            complement(2749..2886)
                     /locus_tag="VF089_15230"
     CDS             complement(2749..2886)
                     /locus_tag="VF089_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175358.1"
                     /translation="MFGGFAAAGKVPSRKLQRAYVLFIVILDLMVGALTVTGTSLPLR
                     R"
     gene            complement(2888..3121)
                     /locus_tag="VF089_15235"
     CDS             complement(2888..3121)
                     /locus_tag="VF089_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX6175359.1"
                     /translation="MPIDRGLIVGGLTGFLGVDGRFLIVPALILFAALPAQKAAGTSL
                     AITTVSAAAGVFRRLRKTLIECSWRRSLLAPAF"
     gene            complement(3111..3512)
                     /locus_tag="VF089_15240"
     CDS             complement(3111..3512)
                     /locus_tag="VF089_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6175360.1"
                     /translation="MSAGTLAAIILSGAVGVSLGALGVGNSYGSGPRVRCCDPGPASD
                     STLVDRRRATSIIGAVAHYWRGNNNVNGGLLFGVSGIAGSFLGSYLTDFFSDHPLLGV
                     FAALMVIVGFAMLRQRIEPGYGTRCSVGFAY"
     gene            complement(3515..>4053)
                     /locus_tag="VF089_15245"
     CDS             complement(3515..>4053)
                     /locus_tag="VF089_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6175361.1"
                     /translation="AHIPGAINVGLGSSFPTWAGTALPGGASIILVIDNDRDLWEASW
                     HLLRIGYDLPKGWLSGGMMAWRTAAKPIEILPQWTVRQLEERLRENPKLLVVDVRQPG
                     EWSAGHIDGALHISGGELPSRIEEVAKDRPVATICGSGYRSSVSASLLLQRGHRDVTN
                     VLGGMSAWKAGGFPVNKD"
BASE COUNT          883 a         1233 c         1121 g          816 t
ORIGIN      
        1 catgacgtta tagacaaatg caaaaaacag attctgtcga atgttgcgca tcgttgcccg
       61 gctcagccgc cgcgccttgg ctattccgcg cagatcgccc ttgaccaagg tgacgtgcgc
      121 gctctcgatg gcgacatcgg tgcccgtgcc catgtcaatg ccgacttgag cttgcgctag
      181 cgctggagca tcgtttacac catcgcccgc catgccgacg atccgccctt catcctgcag
      241 ccgttttatc acttcggcct tctgttcggg caacacttcg gcttccactt gatcgatgcc
      301 caagcgacgc gcgacggctt cagccgtggt ccggctatcg ccggtcagca tgaccatcct
      361 gacaccttct tgatgcaaaa tttggatcgc ttcagccgtc gactgcttga ttggatcgga
      421 caccgctacc agtccggcgg cgcggccgtc aaccccgaca aacataaccg tatggccctc
      481 gcggcgaagt tcctcagcgc gggcgagcag agttcccggc tcgatcgaca gctcctcgag
      541 aagcttgata ttgcccagcg cgaccgagcg tccatctact tttccgataa ctcccctgcc
      601 ggttagcgac cggaactctt ctgcattagc gagccgcaat ccttctctct ctgctgccgc
      661 cacaatagcc gcggcaagtg gatgctcgct ggcacgttcg atactcgcag ccaggcgcag
      721 gatctctttg cgatctgttg gcgcaacgct gacaacttcg gagagctgcg gctttccttc
      781 ggtgagagta ccggttttat cgacgaccaa cgtgttgact ttctctaacg tttccaaggc
      841 ttcggcattt ttgatcagca cgccttccgt agcgccacgg ccgacgccaa ccataatgga
      901 catcggggtt gcgagcccca gagcgcaagg gcatgcaatg atcagtaccg acaccgcatt
      961 gacgatggca tacgccatgc gcggctcagg tccgaatagg gcccaaacga ttgacgtgag
     1021 cgccgcaacc acgataaccg caaccacgaa atacgccgaa acgatatcgg ctagccgctg
     1081 tatcggcgcg cgcgtgcgct gtgcttcgct gaccatccga acgatctgag ccagcaaagt
     1141 ctcgctgccg acacgttgcg cctccatgat gatactgccg gtgccgttga ccgtcgcacc
     1201 ggttacttta cttccttttg ctttctccac cggaatcggc tcgccggtaa tcatcgattc
     1261 atcgatcgaa ctcgcgcctt cgatgacaac gccatcgaca gggacttttt cgccggggcg
     1321 gacgcgcagg cgatcgccga cttggatcag atcgagcaaa acgtcttctt ccctgccgtc
     1381 ctcgtacatc acgcgggcag tcttcggcgc caaacccagc agagctttga tggcgctgct
     1441 ggtttggctg cgcgcgcgca gttcgagcac ttgccccaac aaaacaagcg tggtgataat
     1501 cgcagcggcc tcgaaataaa ccgcaaccgt tccgctatgg ccgtagaacg actcgggaaa
     1561 aatccccggg aagagagttg ccactacgct gtacagataa gacgcgccca cgccgatggc
     1621 gatcaacgtg aacatgttca agcttcgatt cacgatcgat tgccagccgc gctcgaagaa
     1681 cggccaaccg ccccaaagca ccaccggtgt ggcaaggatg aactgaatcc acgtaagcaa
     1741 acgcgaagat agtatgtgct gcaacggctg cccggagatc atatcggaca tcgcaatgat
     1801 gaacagcgga agggaaagtg ccgcgctgat ccagaaccgc cgggtcatgc cgacgagttc
     1861 ggggttgggc gcgtcttcca aggtcactgt tctgggttcg agggccatgc cgcagatcgg
     1921 gcaggatcct ggttcgtcac ggacgatctc cggatgcatg ggacaaacgt actcggtgcg
     1981 tgttgaggac gccgcaagcg tttcaggctc gagcgccatg ccgcacttcg ggcacgcgcc
     2041 cggtttggac tcgcgcactt cagcatccat ggggcagaca taggcggctt catcggatct
     2101 gacgtgaacg tgatttcgcg aagctgtgtg ctcacgagcc ggcgtgggaa caacgtgtcc
     2161 gccgcgctgt tcgcccagcc acttgtcggg atcttctttg aatttctgtg cgcaatgatg
     2221 gctgcaaaaa tagtaaatct ttcccttata ctcctgcttc cccgccgcgc tgcttggatc
     2281 cactgtcatg tcacagactg gatccttgac ggatcgtcgt tggccgctcc cttgctcagc
     2341 gtcggcaacc tgcgacgaag cggctgtttt cacgtactgg gctggatttt ctttgaattt
     2401 ggccaagcaa tgctggctgc aaaaataata gatctgaccg ttgtgctcat gctcgccggc
     2461 agcgcgtgcg ggttccacct tcattccgca aatcggatcg atagccatga tgtcctcctc
     2521 atattctttc cctcagccac gctgacgcaa gaccgccgcg ttaattacac gcatcgttgc
     2581 acgctcaatc agccaatccg gtaggtacat cgaataaccg ttgtagcaac aatcgatcgg
     2641 aacaaaaacc aatggtcagc aggcgcgtaa atgaacatcg cattggcaaa gcagattggg
     2701 aaaattcgat gcagtgcccg gtcgcacaca ggtcgagcgc cagaaatgtc aacggcgcag
     2761 gggtagcgaa gttccggtta cggtaagcgc cccgaccatc aaatctagga tgacaatgaa
     2821 caaaacgtag gctctttgca gcttacggct gggaactttg ccagccgcag caaacccgcc
     2881 gaacattcta aaacgccggg gccagcaaac ttcgccgcca agaacactcg attaaggttt
     2941 ttcggagccg gcgaaaaact cctgcggctg cgcttactgt ggtgattgcc aatgatgttc
     3001 ccgctgcttt ttgcgccggc aatgccgcga acaagatcag cgcgggaacg atcaggaatc
     3061 ggccgtcgac gccgagaaag ccggtcaagc cgccgacgat aagcccgcgg tcaataggca
     3121 aagcccaccg aacagcgcgt tccatatcct ggctcgatgc gctgacggag catcgcgaaa
     3181 ccaacgatca ccatcagcgc agcgaagaca cccaaaagcg ggtggtcaga aaagaaatcg
     3241 gtcaggtatg agcccaggaa ggagccggcg attccgctga caccgaacag caatcctcca
     3301 ttcacattgt tattgccgcg ccagtagtgg gctacagcgc cgattatgct ggtcgcccga
     3361 cgacgatcaa cgagagtgga atcgcttgct gggccgggat cgcagcaacg tacacgagga
     3421 ccggaaccat aagaattgcc gacccccagg gcgcccaagc ttacgccgac ggcgccggat
     3481 aaaatgatcg cggcgagtgt gcccgcgctc attgctaatc tttgttgaca ggaaagccgc
     3541 cggctttcca ggcgctcatg ccgccgagta cgttagtgac gtcgcgatgg ccgcgctgta
     3601 gaagcaaact cgccgaaacc gatgagcgat agccgctgcc gcatatcgtt gctaccgggc
     3661 ggtctttggc cacttcctcg atgcgtgagg gaagttctcc tccgctgata tgcagtgcgc
     3721 catcgatgtg gccggcgctc cattcgcccg gctggcggac atcgacaacc aacagtttgg
     3781 ggttttcgcg taatcgctcc tcaagctgcc gcacggtcca ttgcggcaag atctcgatgg
     3841 gttttgcagc agtgcgccac gccatcattc ctcccgagag ccaccctttg gggagatcgt
     3901 aaccgatgcg caaaagatgc caagaggcct cccaaagatc gcgatcattg tcgatgacca
     3961 gaataatcga tgcgccgccg ggaagcgcgg ttccggccca cgtagggaag gatgacccga
     4021 ggccgacatt gatcgcgccg ggtatgtgcg cgg
//
LOCUS       DASUTG010000661         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_250560, whole genome shotgun sequence.
ACCESSION   DASUTG010000661 DASUTG010000000
VERSION     DASUTG010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250560"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VF089_15250"
     CDS             complement(<1..536)
                     /locus_tag="VF089_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175362.1"
                     /translation="MSFVGAIAAPHSHLQLFTRPAENKDYRAKVARGRKALGVLREQV
                     EKSGADTMLVIHDDHFVNFDFRCYPPFCLLIGKEAKGSGVIDPAEIEKITGAVYKKYL
                     GKTPYSSAPAGMPQHVSDWEGDDKPYVYRVDEDLSVRMLKDLLAMGFDMPFTTNGTMD
                     GELVLTQNFLNPKADKSI"
     gene            complement(767..1903)
                     /locus_tag="VF089_15255"
     CDS             complement(767..1903)
                     /locus_tag="VF089_15255"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175363.1"
                     /translation="MKNGFKIIDSDMHIMEPLDLWEKYIDPRFKERAPRPVRVPTRGD
                     LNLLMIDGKEPRQNSPTLAHDVRMISGRRNNTRGDVIDFARKQNFNAKSQLEAMDIEG
                     IDVAVLFPTACLHIMTMPDMDPLLAEAICRAYNDWLHDFCQENPKRLFGAALLPPHDV
                     SLAVREARRAREKLGFVTAFLRATPLPGQTWFSVYWEPLWAELEETGMTVGFHECTNS
                     GHYPHLERFGMGNRLLRHICTHVTGQMVTMVDMIVGGVLERFPQLKVAFLECNCGWAP
                     SWLQRMDRHWQQLGKHDAPLLTIKPSEYFKRQCVIGCEGDEADIGNVCQLIGDDNLVF
                     SSDYPHSDSDFPKATQEFFHQEMPADSRRKILWDNCIKFYGIDA"
     gene            2045..3061
                     /locus_tag="VF089_15260"
     CDS             2045..3061
                     /locus_tag="VF089_15260"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175364.1"
                     /translation="MATDVRKTAFKRKPRKAMFLSVVMLLFGSAVREAFAGSAAEPTR
                     LRISIPGVGSSSYPLIMAQKKGYFRAEGYEVELIPMAAGLAVKTLLAGEVGVTAAGTV
                     LANLQGAKVRVVMGFIRELPYDLVVPPEIRRVEDLRGKKIAVADRGSVTFFVVRTILQ
                     AHGMEPDRDVTLLNMGTPAVRHQALLAGIVHGVVANFDGTALLADRGYHSIAKAGRFM
                     KGFAGSLSVTQEQLEKRPDDVSRAVRATLKGIRAYLAQRDEAIKYSSAWTKISVDNAA
                     KVHELMSAGALAPDGLLDRETMESVIQESREVAGIKRVVKLDEVFDFRLVQRANDELR
                     SWRP"
     gene            3354..4142
                     /locus_tag="VF089_15265"
     CDS             3354..4142
                     /locus_tag="VF089_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6175365.1"
                     /translation="MDLGLRGKVAVVSGSSDGLGYAVAHSLAQEGARIVLCARREPLL
                     AEARKSIENETGAEVLALTCDVQRLDDVQRLVTEVMQRFGVIHILVNNAGSVPSIRFA
                     DIDDAQWYQMLEGKLLGYIRMTREIAPHMRKAGWGRIINIAGTAGLEPSSTAMAVGLN
                     NAAVMNWTKSLSLTYADDGILVTAVAPGSIETPRQIRNRQREAEITGKSLEEIRQARA
                     QEIPLHRLGRAEEVANVVVFLASERSSYMTGTCVTVDGGVVRGI"
BASE COUNT          926 a         1096 c         1255 g          939 t
ORIGIN      
        1 atgatggact tgtcggcttt aggatttaaa aaattctgtg tcagcaccag ctcgccgtcc
       61 atcgtgccgt tggtggtaaa cggcatgtcg aatcccatcg cgagcaggtc tttgagcatg
      121 cggacgctta gatcttcgtc gacccggtag acgtagggtt tgtcgtcgcc ttcccagtcc
      181 gaaacgtgct gcggcattcc cgcaggcgcc gaactgtatg gagtcttgcc caggtatttc
      241 ttgtagacag cgccggtgat tttctcgatc tccgcgggat cgataacgcc gctgcccttg
      301 gcttctttac cgatcaaaag gcagaagggc gggtagcagc ggaaatcgaa attgacgaag
      361 tggtcgtcgt gaatgaccag catcgtgtcc gcgccggact tttcgacctg ttcgcgcaag
      421 acgccaagcg cttttctgcc tctggcgact ttcgctcgat aatctttgtt ttccgctggc
      481 cgcgtgaata attgcaaatg cgagtgcgga gcggctatgg cgccgacaaa tgacatgatt
      541 ttcgttcaac tccaggtctg cgctgcgcat caatgaagtc gggccggatt acaggtaatg
      601 actcaattgg aaagcagatc cttcacttcg ttcaggatga caactccact caagtgtcat
      661 tctgagcaca gcgaagaatc tgctttactg cgactttttc tgaagcctgt actggtcgac
      721 caaatgagtc actaccggat tacaacactt ttcgacggcc gaaacatcac gcgtcgatcc
      781 cgtaaaattt gatgcagtta tcccacaata tcttcctgcg actgtccgcc ggcatctcct
      841 gatggaaaaa ctcctgggtc gctttgggaa agtccgagtc gctgtgcggg tagtcgctgg
      901 agaaaaccag attgtcgtcg ccgatcagct ggcagacgtt gccgatatct gcttcgtcgc
      961 cttcgcagcc aataacgcac tggcgtttga agtattcact gggcttgatc gtcagcagcg
     1021 gcgcgtcatg cttaccgagc tgttgccagt gacggtccat gcgctgcagc catgaaggcg
     1081 cccagccgca attgcattcc aggaaagcaa ctttcaattg cgggaagcgc tccaacacgc
     1141 cgccgacgat catgtcgacc atggtgacca tttggccggt cacatgggtg cagatatggc
     1201 gcaacaagcg gttccccatg ccgaaccgct cgagatgcgg atagtggccg gaattcgtgc
     1261 attcgtgaaa accgacggtc ataccggtct cttccagctc ggcccaaaga ggctcccagt
     1321 agacgctgaa ccaagtttgt cccggcaacg gcgtggcgcg caggaacgca gttacgaacc
     1381 cgagcttttc tctggcgcgg cgtgcttcgc gcacggcaag cgaaacatcg tgcgggggaa
     1441 gcagcgcggc gccgaaaaga cgcttgggat tttcctggca gaaatcgtgg agccagtcgt
     1501 tgtaagcgcg gcagatcgct tcggcgagca gcgggtccat gtccggcatc gtcatgatgt
     1561 gcagacacgc ggtggggaag agtacggcca cgtcgatgcc ttcgatatcc atcgcttcga
     1621 gctgcgactt ggcgttgaaa ttttgcttgc gcgcgaagtc gataacatcg ccgcgggtat
     1681 tgtttcgcct gccgctgatc atgcgcacgt cgtgggccaa ggtcggacta ttttggcgcg
     1741 gttctttacc gtcgatcatc agcaggttta aatcgccgcg cgtaggtacg cgcaccggcc
     1801 ggggcgcgcg ctctttgaag cgcgggtcga tgtatttttc ccaaagatcc agcggttcca
     1861 tgatatgcat gtcggagtcg atgatcttga atccattctt cattggtctt ccccacttca
     1921 aaatgacgcc gtttcggtta tttccaacta caactagcgc ggaggcgctg tcaaccatta
     1981 ccttgacatg agcgaaccag gagtaataga aattcgcgca gcgagtcact agggagtcgg
     2041 cacaatggca acagacgttc gtaaaacagc atttaagcga aaaccaagaa aagcgatgtt
     2101 tctctcggtc gtgatgctgc tattcggtag cgcagtccga gaggcttttg ccgggagtgc
     2161 cgctgagccc acgcgcttac gcatcagcat tcccggtgtc ggctcatcgt cttatccttt
     2221 gataatggcg cagaagaaag gttatttccg agccgaaggc tatgaagtcg agctgatccc
     2281 aatggcagcc ggactcgcgg taaagacgtt actcgccggc gaagtcggtg tcactgctgc
     2341 gggcactgtg ctggccaact tgcaaggcgc gaaggtgcgc gtggtgatgg gttttatccg
     2401 cgaacttccg tacgatctcg tggtgccgcc ggagatcaga cgggtcgaag atctgcgcgg
     2461 caaaaaaatt gcggtggccg accgcggcag cgtgactttc ttcgtcgtcc ggacgatttt
     2521 gcaagcgcat ggcatggagc cggatcggga tgtcacgtta ctcaacatgg gaacgccggc
     2581 agtgcgtcat caagcgctcc tggccgggat tgttcacggc gtagtggcta actttgacgg
     2641 caccgctttg ctggccgacc gaggttacca ttccatcgcg aaggcgggca ggttcatgaa
     2701 aggcttcgct gggtcgctct cggtgacgca agaacagttg gagaagcggc ctgatgacgt
     2761 gtcccgagcc gttcgcgcta cgctgaaagg tatccgtgcc tacctggcgc agcgcgacga
     2821 agcgatcaag tacagttcgg catggaccaa gatctcagtg gacaatgcgg cgaaagttca
     2881 cgagctgatg tcggccggcg cattggcgcc ggatgggctg ctagatcgcg agaccatgga
     2941 atccgtgata caggaatccc gtgaagtcgc agggattaaa cgcgtggtca agctcgacga
     3001 agtcttcgac ttccgccttg tccagcgcgc caatgacgaa ctgcggagct ggcgaccata
     3061 gtaaacgagc gcaaacgcgt tgctttaaag ttgcgaaaaa caaaatcgct gcgaacattg
     3121 tcgcgatgga ccggtccggt ctcatgtttt tctcgcttat gcgcgatcga agcagacgac
     3181 tcgcgcacac gttttgagac cgcggcagta tgctaaggca gcgcaaaaaa tatggttgtt
     3241 atcatccgaa tggatgtaca gtaacggtta tcgaaaaatt tggttaaacg ataggacgct
     3301 tggaaccatc gcaatgatcg gcgaatgata cagtaaacgg gaaaaggtta acgatggatt
     3361 taggattgcg cgggaaggtg gctgtcgtga gcggttccag cgatggtctt ggctatgctg
     3421 tggcgcattc tttggcgcag gagggcgcgc ggattgtcct gtgtgccaga cgtgagccgc
     3481 ttctcgccga ggctcggaaa tccatcgaga acgaaactgg agccgaagtc ctggcgctaa
     3541 cttgtgatgt tcaacgtctg gacgatgtcc aacgtttagt caccgaagtc atgcaacgct
     3601 tcggggtgat tcatattttg gttaacaacg caggaagcgt tccgagtatc cgctttgccg
     3661 atatcgacga tgctcagtgg tatcagatgc tagaaggaaa attgctcggc tatattcgga
     3721 tgacccgaga gattgcgcca cacatgcgca aagccgggtg gggccggatc atcaacattg
     3781 cgggaaccgc cggattggag ccaagcagta cggcgatggc agtgggtctg aacaatgccg
     3841 cggtgatgaa ctggacgaaa tctttgtctc ttacttatgc cgatgacggt atcctggtga
     3901 cggcggtcgc gccgggttcg atcgagacgc cgcggcaaat tcgcaatcgc caacgtgagg
     3961 cggagataac cggcaagagt cttgaggaga tccgccaggc gcgtgcgcaa gaaatcccgc
     4021 tgcaccggct cggccgagcc gaggaagtag cgaatgtcgt tgtctttctt gcttcagaac
     4081 gctccagcta tatgaccggc acctgcgtga cggtagatgg cggggtagtg cggggaatct
     4141 gatcggtaga ggaacgatgc tcattcaaga gcgggatgtc gctgcgaaaa cgttgcgtca
     4201 tgagacctcg cgaggc
//
LOCUS       DASUTG010000662         3541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1138899, whole genome shotgun sequence.
ACCESSION   DASUTG010000662 DASUTG010000000
VERSION     DASUTG010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138899"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VF089_15270"
     CDS             complement(<1..1309)
                     /locus_tag="VF089_15270"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6175366.1"
                     /translation="MIQSDEEKSAAAGGANDFEAMFEESLRSVKPGGIVKGMVVGITA
                     THVLIDVGYKSEGQIPIHEFQDRQGNLEVKVGEEVDVYFDSSENEGGGINLSRQRAQS
                     MKVWEVIEKAFNDGTPVEGTITGKVKGGFKVDVGGVMGFLPGSHVDIRPTRNLDKFLG
                     TKDSFAVLKFNRPRGNVVLSRRALLEKERDVLKQEVLKVLEEGVILEGTVKNITGYGA
                     FVDLGGIDGILHISDMSWGRISHPSELLQVGQKVNVVVLKFDSEKERISLGMKQLMTD
                     PWHSVAEKFPIGMRVQGKVISIMDYGAFVELESGIEGLIHISEMSWTRKIAHPSKILQ
                     VGQSVEVVILNVDPEHRRISLGLKQVMPNPWEEAKQKYPVGSVVKGPVRNITDFGIFV
                     GIEEGIDGLVHVSDLHWTKKVKHPSELFKKGDIVEAKVLGVNVE"
     gene            complement(1306..1998)
                     /gene="cmk"
                     /locus_tag="VF089_15275"
     CDS             complement(1306..1998)
                     /gene="cmk"
                     /locus_tag="VF089_15275"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX6175367.1"
                     /translation="MIVAIDGPSGAGKSTLAKRVARELRFTYLDTGAIYRALALKILR
                     RGVDPSDTAALSAIVIDSDIDLRASNGQLQVFLDGEDVSASIRTPEVSQMASKASAIP
                     MVRHRLLDLQRELGRRGNVVAEGRDIGTVVFPDAQVKIYLDASIEERARRRCDELRAA
                     GREVSLRETINEMEERDKRDSERDIAPLRKANDAFALDSSGLTADGVAEKVLQLIKSE
                     SSKLKNDRSLGI"
     gene            complement(2035..3321)
                     /gene="aroA"
                     /locus_tag="VF089_15280"
     CDS             complement(2035..3321)
                     /gene="aroA"
                     /locus_tag="VF089_15280"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX6175368.1"
                     /translation="MKRVLQSKKPLRGEISVPGDKSIGHRAVIFSSIAYGSSRVSNLS
                     GGEDNQRTVQAFKDLGVKISTEGETLYIEGKGWEGLSCPKNIIDCGNSGTTMRLLSGV
                     LAGRPFASILDGDQSLRRRPMQRVIEPLRRMGANLVGREGTGLAPLEINGGKLRGIEY
                     PMPMASAQVKSAILLAGLQAEGMTVVDEPQNSRNHTELMIKGFGGEVAVHGRKVSVNG
                     GQTLHGKDLRIPGDISSAAFFLVAAALIPGSKVVVRRVGVNPTRDGIIEILRRMGADI
                     ELLGATVEAGEPVADLSVTGRRLKGVKIGAELVARTIDEYPILAIAAALADGVTMISD
                     VKELRFKESDRISAMTEGLRRLGVEVEEREDGMTIHGRKTLQGNKIRSFGDHRIAMSL
                     AVAGLCADGGVEIDDAQCVDISFPTFFDLLDEICLP"
     gene            complement(3335..>3541)
                     /locus_tag="VF089_15285"
     CDS             complement(3335..>3541)
                     /locus_tag="VF089_15285"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX6175369.1"
                     /translation="GFKDFTRIASSRPELWRDICLMNRQALGQSLGDYINSLEQLRRW
                     IAAGKGASLEKEFARANEIRQQIA"
BASE COUNT          762 a         1077 c          845 g          857 t
ORIGIN      
        1 tttcgacgtt gacacccaat actttggcct caactatgtc gccctttttg aaaagttcgg
       61 atggatgttt gacctttttc gtccagtgca aatccgaaac gtggaccaaa ccgtcgatcc
      121 cttcctcgat gccgacgaaa atcccgaaat cggtaatatt ccgcaccggt cccttaacca
      181 cgctgccgac gggatatttt tgtttcgcct cctcccatgg gttcggcatc acctgcttca
      241 aacctaatga aattcgccgg tgttccggat cgacgttgag aatcacgact tcgaccgatt
      301 gcccgacttg gaggatctta gaggggtgcg caatttttcg cgtccacgac atctcggaaa
      361 tatggatcaa cccttcgatg ccgctttcca gctcgacaaa agccccatag tccatgatgc
      421 tgataacctt gccttggacg cgcattccaa tggggaattt ttccgccaca ctgtgccatg
      481 gatcggtcat caactgcttc atgcccagag aaatccgctc tttttcggaa tcgaacttca
      541 gcacaaccac gttcactttt tgacccacct gaagaagttc cgagggatgg ctgatacgtc
      601 cccaggacat gtcgctaatg tgcaggatac cgtcgatgcc accgagatcg acgaaggcgc
      661 catagcccgt gatatttttc acggtacctt ccagaataac accttcctcg aggaccttta
      721 gcacctcctg cttgagaacg tctcgttctt tctccagtag ggcccggcgg gagagcacga
      781 cgttgccccg ggggcggttg aatttaagca cggcgaagga atccttggtg ccgaggaatt
      841 tatctaaatt gcgcgtcggc ctgatatcga catgcgatcc gggcaagaat cccatgactc
      901 cgccgacgtc aactttaaat ccgcctttga ctttgccggt tatcgttcct tcgacagggg
      961 tgccgtcatt gaatgctttt tcgatgactt cccagacctt catgctctgg gctcgttgcc
     1021 gcgacaaatt gatgccccca ccttcgttct ctgaagagtc aaagtagacg tcgacctcct
     1081 cgccgacctt tacttcaaga ttgccctgac gatcctggaa ctcatggatc ggaatttgcc
     1141 cttcggattt gtagccgaca tcgattaaga cgtgcgtcgc tgtaattccc acgaccattc
     1201 ccttgacgat gccgccgggc ttgacagaac gcaggctctc ctcgaacata gcttcgaaat
     1261 cattggcgcc tcccgccgcg gcgctttttt cttcatcact ctgaatcata tacccaaact
     1321 ccgatcattt tttaattttg aagactcact tttgattagt tgcaatacct tctcagccac
     1381 cccatccgca gtcagccctg atgagtccag cgcaaatgcg tcattggctt tgcgtaaagg
     1441 cgcgatgtcg cgttcgctgt cccttttgtc tcgctcctcc atctcgttga tcgtttcgcg
     1501 aaggctgacc tcccgtccgg cagcgcgcaa ttcgtcgcaa cggcggcggg ccctctcctc
     1561 gatggatgca tcaagataga tcttaacctg agcgtccggg aagacgacgg taccaatgtc
     1621 gcgaccttct gccacgacat tgccgcgccg cccgagttcg cgctggagat ccaaaagccg
     1681 gtgacgcacc attggaatgg cggacgcttt ggaggccatc tgactaactt ccggagttcg
     1741 aatcgaggcc gacacatcct cgccgtcgag aaatacctga agctgaccat tgctcgcgcg
     1801 aagatcgata tcgctatcga ttactatagc actcaatgcg gcagtgtcac ttggatccac
     1861 tccccggcgc aatatcttca aggcaagcgc tcggtaaatg gcgccggtat cgaggtaggt
     1921 gaagcgtaac tccctggcca cacgttttgc cagcgtgctt tttcccgctc cggagggacc
     1981 atcaatcgct acaatcattt acgtcccact tgacctacta tcgctaacaa accgttatgg
     2041 caaacaaatc tcatcgagca aatcgaaaaa cgtcgggaac gatatatcga cgcactgggc
     2101 gtcgtcaatt tcaacgccgc cgtcggcgca cagtccagcg acagccaatg acattgcgat
     2161 gcgatggtca ccaaagctgc gaatcttatt tccttgcagc gtcttgcgcc catggattgt
     2221 catgccatct tcgcgttcct cgacctcaac tccaagcctg cgcaatcctt cggtcatggc
     2281 cgaaatgcga tctgactctt tgaagcgaag ctccttgaca tcacttatca tcgtaacacc
     2341 gtccgccagc gcagctgcta tcgccagaat gggatattca tcgatagtgc gcgccaccag
     2401 ttccgctcct atcttgaccc ccttaaggcg cctgccggtg acgcttagat cagccaccgg
     2461 ctcgccggcc tcgaccgtgg cgccaagcag ttctatgtcc gcccccatgc gccgaaggat
     2521 ctcaatgatg ccatccctgg tcggattgac tccgacccgg cgcacgacaa ctttcgatcc
     2581 aggtatcagt gcggccgcga ctaagaaaaa tgccgccgag gaaatgtccc cgggtatgcg
     2641 caggtctttt ccatgaagcg tttggcctcc gttgaccgaa accttccggc catgtaccgc
     2701 gacttcaccg ccaaaacctt taatcatcaa ttcggtgtgg tttcgcgagt tttgcggttc
     2761 gtcgacaacc gtcattcctt ctgcctggag accggccagt aaaatagcgg atttcacctg
     2821 ggcgctggcc attggcattg gatactcgat ccctctgagt ttccccccgt taatctcgag
     2881 cggcgccaat cctgtgcctt cacgcccgac gagattagcg cccatacgcc ttaacggctc
     2941 gatcaccctt tgcatcggcc ggcgccgtag cgactgatca ccgtcaagta tcgaggcaaa
     3001 cggacggccg gccaaaaccc cggaaagcaa ccgcatcgtc gtgcctgagt tgccgcaatc
     3061 aatgatattc ttggggcaag acaatccttc ccagcctttg ccttcgatat ataacgtttc
     3121 gccttcggtt gagatcttca caccgaggtc tttgaatgct tggacggtgc gctggttgtc
     3181 ctcgccgccg gaaagattgc tcacccgact ggaaccgtag gcgatactgc taaagataac
     3241 ggcgcgatgc ccgatcgatt tatctccggg aaccgagatt tctccgcgca gaggtttctt
     3301 gctttgtagg actcgtttca ttgtcaccca agcgtcaggc gatctgctga cgaatctcat
     3361 tggcgcgggc aaactctttc tccaaagacg cgcctttacc tgcggcaatc caacgcctca
     3421 gctgctccaa actgttgatg tagtcgccga gcgattggcc cagggcctgc cgattcatca
     3481 aacagatatc gcgccacaac tctggccggc ttgaagcgat acgagtgaaa tctttgaagc
     3541 c
//
LOCUS       DASUTG010000663         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1883078, whole genome shotgun sequence.
ACCESSION   DASUTG010000663 DASUTG010000000
VERSION     DASUTG010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883078"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VF089_15290"
     CDS             <1..783
                     /locus_tag="VF089_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6175370.1"
                     /translation="YAGYLRRCSREEFASLRYAIAGAEKLREQIAGSYKEKYGIDLLE
                     GYGCTELAPVVSVNVPDVIDGGEKQIGNKPGSVGHPIPGIAVKVIDPETELPLDCGQE
                     GLLLVKGPNCMLGYLGQPDLTAQVFRDEWYVTGDIATIDEDGFIRITDRMSRFSKIGG
                     EMVPHMRIEEVINQVLGSAAAVVTALPDEQRGEKLIAFYTHNGISRDELWNRLNQSAL
                     PKLWIPKRENLYTLESIPLLGSGKIDLKQVKALAQQQAGKAH"
     gene            928..1908
                     /gene="bioB"
                     /locus_tag="VF089_15295"
     CDS             928..1908
                     /gene="bioB"
                     /locus_tag="VF089_15295"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX6175371.1"
                     /translation="MDFGKLADKSLQGELLERDEMKAVLNAADEQIPELVSAAFRVRH
                     HYFGKRVQIHMLQNAKSGLCPEDCHYCSQSSVSEAPIERYPFLSKEQLVEGAKRAKEA
                     GAVRYCIVNSGRGPTHKEVEQIAEAVKEIRAKVGINICCSLGLMTEDKIRRLKEVGVG
                     RINHNLNTSREHHPEIVTTHTYDDRVATVENVKAAGVGTCSGGIIGMGESDDDIINLA
                     ITVRAMDIESIPLNFLHSIPKTPFEAKRQLTPQKCLKTLCLFRFVNPDKEIRVAGGRE
                     INLRSLQPLSLYPANSIFVNGYLTTPGQEASDAHRMIEDLGFELDQPAAI"
     gene            complement(1928..2659)
                     /locus_tag="VF089_15300"
     CDS             complement(1928..2659)
                     /locus_tag="VF089_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175372.1"
                     /translation="MSLSSLHLDLAPTGTLRVGINYGNPVLATKDPNSGRLSGVAVDL
                     ARELGRRTDLPLELVGFESAGKMVEGLKAAAWDVAFLAVDPGREQEISFTAPYIEIEG
                     AYLVPPGSALRAIADVDHENVRVGVSSKSAYDLFLSRNLKHAELVRESSPEAAFELIV
                     AGKVDALGGVKQHLVARMEKLPGSRVLEGRFMAIQQALGIPKAREAGLSYLREFIEDA
                     KASGLVARALEKAGVQGVSIAPTMT"
     gene            2931..>3592
                     /locus_tag="VF089_15305"
     CDS             2931..>3592
                     /locus_tag="VF089_15305"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX6175373.1"
                     /translation="MQLEQFAAQIRTEHDIVFFTGAGISTESGVPDFRSPGGVWTKYQ
                     PVYFQDFLVSEAARIQHWRLKKATYELFKTVKPNIGHYAIRDFEQRGQLLGLITQNID
                     GLHKLAGIPEERLVELHGTDRLVTCLGCGKRFEPADIYEILAEEFSAPTCDACGGFLK
                     SANISFGQQMPAEPMRRAQIWTEAADVFIVVGSSLQVQPAASFPVIAKRNGALLAIVN
                     RDP"
BASE COUNT          877 a         1004 c          947 g          764 t
ORIGIN      
        1 tatgcgggct atttgagacg ctgcagccga gaggaatttg ccagcctgcg ctatgctatt
       61 gccggcgcag agaagttacg cgaacagatt gccggaagtt acaaagagaa atatggtatt
      121 gatctgctcg aaggttacgg ctgcaccgaa cttgcaccgg ttgtgtcggt gaacgttccg
      181 gacgtgatcg acggcggaga aaaacaaatc ggcaataagc caggtagcgt cggacatccg
      241 attccgggca tcgcggttaa ggtgatcgat cctgagacgg agctgcccct agactgcggt
      301 caagagggtt tgcttctcgt taaaggcccc aattgcatgc tcggttatct cggccagcct
      361 gacctcacgg ctcaagtctt ccgggatgaa tggtatgtaa ccggtgatat agcgaccatc
      421 gacgaggacg ggttcatacg aatcaccgat cgcatgagtc gtttcagcaa gatcggcggc
      481 gaaatggttc cacacatgag gatcgaagaa gttatcaatc aggttcttgg cagtgccgca
      541 gctgtggtca cagcgctgcc ggatgagcag cgcggcgaga agctgatcgc cttttacacc
      601 cacaacggca tcagcagaga cgaactctgg aacaggctca accaatcggc actgccgaag
      661 ctctggatcc ccaaaagaga gaatctctac acacttgaat cgattccact gctcggctcg
      721 gggaaaattg atctgaaaca agttaaggcg ctagctcagc aacaagctgg aaaagcccat
      781 tgaagcctgt cgcttggcat tcctcatcga tcacagttaa gcagacaatt agattccaag
      841 atgaaggctc tctaatcccc tcaagataat aacccaagat tgcaagggtt tccgacgcat
      901 gctagaaatt gcacaccgga ggggataatg gattttggca aactggctga taaatcgctc
      961 caaggtgagc tcttggaacg ggatgagatg aaggctgtgc tcaacgctgc cgacgaacag
     1021 attccggagc tcgtcagcgc agcttttaga gtgcgccatc actacttcgg caagcgggtg
     1081 cagatccaca tgctgcaaaa tgccaaaagc ggtttatgcc cggaggactg ccactactgc
     1141 tctcagtcgt cggtttctga ggctcccatc gagcgttacc cgttcctttc caaagaacag
     1201 ctggttgaag gcgccaagcg ggccaaagaa gcaggcgcag tgcgctactg cattgttaac
     1261 agcggccgcg gcccaaccca taaagaggtc gaacagatcg ccgaagcggt aaaagaaata
     1321 cgcgcaaagg tcgggatcaa catctgctgc tcgctgggtt tgatgaccga ggataaaatt
     1381 cgcagactta aagaggtcgg cgttggccgg atcaatcata accttaatac cagccgcgaa
     1441 caccatccgg aaatcgtgac aacccacacc tatgacgacc gcgtcgcgac ggtcgaaaac
     1501 gtcaaagccg caggtgtcgg tacatgctcc ggcggcatta tcggcatggg tgaaagcgat
     1561 gacgacatca tcaatcttgc gataacagtc cgagctatgg atatcgagtc catcccgcta
     1621 aactttctgc attcgattcc caaaactcct tttgaagcca agaggcagct aactccgcaa
     1681 aagtgtttga aaacgctatg cctgttccgt ttcgtgaatc cagataaaga gattcgcgtg
     1741 gcgggaggaa gagagatcaa tctgcgctcg cttcaaccgc tcagtctcta cccggcgaat
     1801 tcgattttcg tcaatggtta cctgacgacc ccaggtcaag aagcgtccga cgcgcatcgc
     1861 atgatcgaag atctcggctt cgagctcgat caaccggcgg caatctaacg acagccgact
     1921 cgcttactta agtcatcgtt ggggcgatcg agacgccctg aacaccagct ttctcgagcg
     1981 cccgggcaac cagcccggaa gcctttgcgt cttcgatgaa ttcacgaagg tagcttaacc
     2041 ccgcctcacg cgcttttgga ataccgagcg cctgctgaat cgccatgaag cggccctcga
     2101 gcacccgcga cccgggaagc ttctccatac gcgccaccag gtgttgtttc acaccgccga
     2161 gcgcgtcaac tttgcctgca acgatcaatt cgaacgccgc ctcagggctg gactcgcgca
     2221 ccagttcagc gtgttttaga ttacggctca aaaacaaatc atatgcgctc ttacttgaaa
     2281 caccaacgcg aacattctcg tggtcaacat ccgcgatcgc gcgaagcgcc gacccgggcg
     2341 gcacaaggta ggcgccttcg atttctatgt acggggcagt aaaactgatt tcctgttcgc
     2401 gtcctggatc gaccgccagg aatgcaacat cccaagcagc ggctttcaat ccttcaacca
     2461 tttttccggc cgattcaaag ccaaccagct cgagcggcaa atcggtccgt ctgccaagtt
     2521 cgcgcgccag atcgacggct acgccactca gccgtccact gtttggatct ttcgtcgcca
     2581 gcacgggatt tccatagttg atgccgacgc gcagtgtccc tgtcggcgca agatcaagat
     2641 gaagagagga taagctcata gcatggctgc cttaccttag tggatttgcg acgaagttac
     2701 tggttaggag aaaagcggtc aagctcaatg ctgtcatgcc gtcgaacgct ctcgtaggag
     2761 atccggtcga gtgcggttga cgagtgagta agcgtatttg gattcggatc gcagcttttc
     2821 atctagccgc cgctcccatg ctgatactga ctcgaaacat tgagttcaat cacttcaact
     2881 tatatgcttc ttccatgcgc cgcagcggac aatctgattg aggtcacgag atgcagctgg
     2941 aacaattcgc cgcgcagata cgaaccgagc atgacattgt ctttttcact ggcgcgggga
     3001 ttagcaccga gtccggcgtt cccgattttc gcagcccggg cggtgtctgg acaaaatatc
     3061 aaccggtcta ttttcaagat ttcctagtca gcgaagccgc gcgcattcag cattggcgct
     3121 tgaagaaagc gacttacgag ctcttcaaaa ccgtcaagcc gaatatcggt cactatgcga
     3181 tccgggattt cgagcagcgc gggcagctac ttggcctcat cacacaaaac atcgacggcc
     3241 tgcacaagct cgccggtatt ccggaagaaa ggctggtcga actccacggg accgaccgcc
     3301 tggtcacctg cctcggttgc ggcaagcgct ttgagcctgc cgacatttat gaaatcttgg
     3361 cggaagagtt ctcggcgcct acctgcgatg cttgcggcgg ctttctcaaa tcagcgaaca
     3421 tctccttcgg tcaacaaatg ccggccgaac ccatgcgccg agcgcaaatc tggaccgagg
     3481 cggcggatgt tttcatcgtc gtcgggtcct cgctacaagt ccaacccgcc gcctcttttc
     3541 ctgttatcgc caaacgaaat ggagccctgc tggcgatcgt caatcgcgat cc
//
LOCUS       DASUTG010000664         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1286959, whole genome shotgun sequence.
ACCESSION   DASUTG010000664 DASUTG010000000
VERSION     DASUTG010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286959"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF089_15310"
     CDS             <1..224
                     /locus_tag="VF089_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEX6175374.1"
                     /translation="QSGINCALSNTAGSMVGDAAAVHLAASMPGISPLCELGEFEVIS
                     GDPFFGLAVEKGTIQVPEGDGLGVSLRGF"
     gene            249..950
                     /locus_tag="VF089_15315"
     CDS             249..950
                     /locus_tag="VF089_15315"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6175375.1"
                     /translation="MSQSEFAAAVDKIVGDYNDRLKWVSFSKDHLTREAAQVLIEQWS
                     NFTRHSRQCWAHVVGNCPIIEVRKFIVTENLYEEEAQEGHSHFEILVRMGMALGLTRH
                     DIEFAKPLPTTVVAMNAWEAMTKNRSWYEGLAAKSVLERTNNPNCGNFSHHQADYWTR
                     QLNLSKEDTEFWWMHDSVDQIHGDGSLKLLEKYLTRDDQKQAALRAAEESMMAWTVYF
                     DGFYYEGLRRSAASR"
     gene            1107..2201
                     /locus_tag="VF089_15320"
     CDS             1107..2201
                     /locus_tag="VF089_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175376.1"
                     /translation="MAKAETKKTLQQFDEELKLQYISGQWLYEGLLTACIGGPKPRGE
                     AFIWPWKTVHEKLLEACDVLEESFTARRSLLFNNPGLPTGGTTHTLLMGIQMIKPGEI
                     AWAHRHTLAAIRFVVHGDGKVFTVVDGEKCPMETNDLILTPQWTWHDHQNPTGESAVW
                     VDVLDVPFLLGLNQPFYEPYPGNKVQAVREKSSDHLQHRTGWVRPSWEDAKQRNIPLR
                     YRWKDIEPQLRILADSPGSLYDGVALEYVNPLSGGPTLATLSCWIQLLRPGERTRKHR
                     HTSSAVYFVVGGDGTTVVNDKAMDWTEHDSFAVPNWAWHEHANRSKTEEAILFSVNDT
                     PIVRAFDLYREEPENSLHTMQTPQVPAVKK"
     gene            2359..3792
                     /locus_tag="VF089_15325"
     CDS             2359..3792
                     /locus_tag="VF089_15325"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175377.1"
                     /translation="MPDLRTWLSKVDKMGQLITVENVDWDLELSTLTEIINERSRIRP
                     AIVFDSIKGYPDGYRVAVNLLSSLQRLALTMGMDPGISEFDFVQRWRRQVKSIQPIEP
                     RKVKNAPLFENVQKAGEIDLLKFPVPRWHELDGGRYVGTDDLVVTRDPEENWVNVGTY
                     RIMIHGSDHMGLHMSPGKHGRVHRDKYHQAGKPLPIAVCFGHHPINFLVASTDVPNQI
                     NEYAYAGGITGKALDIVDGPLTGLPLPADAEIAIEGEVLPNDFMPEGPFGEWTGYYAS
                     NQAAVPVIRVKAVYHRNDPILCGFPLLKPSSGDNLHFSLMRSALIWNALDEAGVPDVQ
                     AVWAHPSGGRFMTVLSIRQRYPGHAKQAAMIASQCRSGAYLGRYVIVVDDDIDITNSE
                     ELIWAISSRSDPVQSIDILRRCWSGPLDPRIPAEEKGFSSRAIIDATRPYEWRNKFPK
                     VSGASRELKSEVGKKWNGFLDDKIPNQ"
     gene            3908..4885
                     /locus_tag="VF089_15330"
     CDS             3908..4885
                     /locus_tag="VF089_15330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175378.1"
                     /translation="MKIRTFMIISLLLLASFSFQPAVLAQGAPERIRISYSSGGMTSI
                     DLFIARDRKYFQQQNLTGELIRMSANLAISAGISGDVDVLGSIGSAIRSIQRGAPLRV
                     ISVTLRRPLFFLVARPEYSTVKDLKGKTLGIVTFGGSQHTTAKRLIALHGLNADKELT
                     SIQVGEESMQLQALTSNAIQVAAISPPYAQIARDKFNMKILETSTDKFASIQNGAAVN
                     LKMLQERPDFLKRFLRARAQANKYFLEREKEASELLASIWNSDFKIANESYRASKAAF
                     TGTGIPTDDEIKEYLALDAQIIGLPQPVPAASVFDFTLQREINKELGIK"
     gene            complement(4937..5506)
                     /locus_tag="VF089_15335"
     CDS             complement(4937..5506)
                     /locus_tag="VF089_15335"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX6175379.1"
                     /translation="MKIYVSLGLLLTLILIVEPGKLPAADESNLSSEDRSLTPESIGR
                     KLRELLDRLKENFAFEIWAEDRAPISLMLRNRERLGLTVEQVKHLEQLRNDFAKESIR
                     SEANLRIAELDLTSLLEAQPVDMTRVEAKIRDIERIRADLRIARVRSIEKGKAQLSTE
                     QRRKLQELEADQNFAGMRFKNELPARLKQ"
     gene            complement(5759..>6023)
                     /locus_tag="VF089_15340"
     CDS             complement(5759..>6023)
                     /locus_tag="VF089_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175380.1"
                     /translation="APFALLTLNVQNSFNCPLFASRSILGHTLATEHFADERLLQSLR
                     DYLPSGNLLKRSDSSSTARTSSSGRILGPQAAGFDDPVEFRLA"
BASE COUNT         1427 a         1607 c         1658 g         1331 t
ORIGIN      
        1 aacagtcagg cataaactgc gcgctatcca ataccgccgg tagcatggtg ggcgatgctg
       61 cggcggtcca tctggccgcg agcatgccgg ggatatcgcc tctgtgcgag ctcggggaat
      121 ttgaagttat ttccggagac ccattttttg gcctcgcggt cgaaaaaggc accatccagg
      181 tacccgaagg cgacggcttg ggtgtgagtc tgagaggatt ttaaagtggg gagttccgga
      241 ggatcttcat gagccaatct gaatttgccg cggccgtcga caagatcgtc ggcgactata
      301 atgatcgtct caagtgggtg tcattttcca aagatcatct aacccgtgag gcggctcagg
      361 tattgatcga gcaatggtca aatttcaccc gacatagccg gcagtgctgg gcgcatgtcg
      421 ttgggaactg cccgattatc gaggtgcgta aatttatcgt cacggaaaat ctttatgagg
      481 aggaggccca ggagggccac tcgcattttg aaattctcgt gcgcatggga atggctttag
      541 ggctgacgcg acacgatatt gagttcgcca agcccttgcc gacaaccgta gtggcgatga
      601 atgcctggga agcgatgacc aaaaatcgct catggtacga aggtctggcg gccaagtccg
      661 tgctcgagcg aactaacaat ccgaactgcg gtaacttttc ccatcatcag gccgattact
      721 ggacccgtca gttaaacctg tccaaagagg acacagagtt ttggtggatg cacgattcgg
      781 tggatcagat tcacggcgac ggctcattga aacttttgga aaaatacttg acacgggatg
      841 accagaagca ggcggcgctg cgcgcggcgg aagaatcgat gatggcttgg acggtctact
      901 tcgatggttt ttattacgaa gggctccgaa gaagcgccgc gagccgatga ttagacgcgt
      961 tcaaatagtt caagacgttg aagccgttca aatcgtctct gagttaacgg atgacaacag
     1021 ggtacttatc cgaagcaacg gtttgaacgt tttgaactaa ttgaacggct tgaacaggag
     1081 cgaagcgact agtaatggag tgagctatgg caaaagccga aaccaagaaa acccttcagc
     1141 aattcgacga ggaactcaaa cttcaataca tcagcggtca gtggctttat gaagggctgt
     1201 tgaccgcgtg catcggcggc cccaagccgc ggggcgaagc cttcatctgg ccgtggaaaa
     1261 cggtgcacga gaaattactc gaggcctgcg acgtcttaga ggagagcttc acggcgcggc
     1321 gcagtctgtt attcaacaac cccggtctcc caacgggcgg cacaacccat actttgctca
     1381 tgggtattca gatgatcaag cccggcgaga ttgcctgggc acaccgccat acgttggcgg
     1441 cgattcgttt cgtcgtccat ggcgacggca aggtctttac cgtggtcgat ggcgagaaat
     1501 gccccatgga gacaaatgat ttaattctca cgccgcagtg gacctggcac gatcaccaga
     1561 atcccacggg agaaagcgcc gtgtgggtcg acgtgctcga cgtgccgttt ctgcttggcc
     1621 tcaatcaacc gttttacgaa ccctatcccg gcaacaaggt acaagccgtc cgcgaaaaat
     1681 cgagcgatca tctgcagcat cgcacgggtt gggtgcggcc gtcctgggaa gatgccaagc
     1741 aacgcaacat cccgctgcgc taccgttgga aagacattga gccgcagttg cgcatattgg
     1801 cggatagtcc cggaagtctc tatgatggcg tggcgttgga atatgtcaat ccgctgagcg
     1861 gcggtccgac cctggcgacg ctcagttgct ggatccagtt actgcggcct ggcgagcgca
     1921 caagaaagca tcgtcatact tcaagcgcgg tttattttgt agtcggcggt gacggcacga
     1981 cggttgttaa cgataaggcg atggattgga cggagcacga ttcctttgcg gtgccgaact
     2041 gggcatggca tgagcatgcg aaccgttcaa agacggaaga ggcgattctg ttttcggtta
     2101 acgacacgcc aattgtccgg gccttcgatc tgtaccgtga agaaccggag aattcgctac
     2161 atacgatgca aacgccgcaa gtgccggcgg taaagaagta aaaaccagtt cgaaccgttc
     2221 aaaccgttct accggttcca acccccagct ctgttccttc gccgagactc gcgggagaat
     2281 gaaggagagg agttgaacgc agtgtagcgg ttgaacggct tgaactagtt gaactcccaa
     2341 aacggaagaa gaatacccat gccggattta agaacatggc ttagcaaagt cgacaagatg
     2401 ggccaattga tcaccgttga aaacgttgat tgggatttgg agctatcgac gttgacggag
     2461 atcatcaacg agcgatccag gattcgtcca gccattgtct tcgatagtat caagggatat
     2521 cccgacggct accgcgtcgc cgtcaacctg ctgtcgtcgc ttcaacggct cgctctgacc
     2581 atgggcatgg atccgggcat cagcgagttc gatttcgttc agcgatggcg ccgccaggtc
     2641 aaatcgattc agcccattga gccgcgcaaa gttaagaacg cgccgctgtt tgaaaatgtt
     2701 caaaaagccg gcgagatcga tcttctcaag tttccggtgc cgcgctggca tgagctcgat
     2761 ggcgggcgtt acgtcggtac ggacgatctc gtggttaccc gggatcctga agagaactgg
     2821 gtcaatgtcg gtacctatcg gataatgatc cacggatccg atcatatggg tctgcacatg
     2881 tcgccgggaa aacatgggcg cgtccatcgc gacaaatacc accaagccgg caagcccttg
     2941 cctattgcgg tttgcttcgg tcatcatccg atcaactttc tggttgcctc aacggacgtg
     3001 ccgaaccaga tcaacgaata cgcttacgcc ggcggcatta ccggcaaagc gctcgacatc
     3061 gtcgacggac cattgaccgg attgccgcta ccggcggatg ccgaaatagc cattgaaggc
     3121 gaagtgctgc cgaacgattt catgccggaa ggaccattcg gcgaatggac gggctactac
     3181 gcgagcaatc aagcggcggt gccggtgatt cgtgtcaaag cggtgtacca ccgcaatgat
     3241 ccgattctgt gcggcttccc attgttgaag ccttccagcg gcgacaacct gcacttttcg
     3301 ctgatgcgct cggcgctgat ctggaacgcg ctggacgaag ccggagtccc ggacgtgcaa
     3361 gccgtatggg cgcacccgtc gggcggccgc ttcatgaccg tcttgtcgat ccgccagcgt
     3421 tatcccggcc acgccaaaca agctgcaatg attgccagcc agtgccgctc cggcgcgtat
     3481 ctcggccgct acgtcattgt cgttgacgac gacatcgata tcaccaattc ggaagaactg
     3541 atctgggcca tcagctcgcg ctccgatccg gttcagtcga tcgatattct gcgccgctgc
     3601 tggagcggac cgttggaccc gcgaattccc gccgaagaga agggtttcag ctcgcgagcc
     3661 atcatcgatg cgacccggcc ctatgaatgg cgcaacaaat ttcccaaggt gagcggcgcc
     3721 agccgagagt taaagagtga ggtaggtaaa aagtggaatg gcttcctcga tgacaagatc
     3781 cccaaccaat aggctgtggg cagcgactca acgccagttt caaaaatacg catacctgaa
     3841 taagaacggt ccgaagcccg tggtttgaca gctcggaaga tgtactttgc gaagggtgga
     3901 tgcatttatg aagattagaa ctttcatgat tatttcactt ttgctgctgg cttctttctc
     3961 ttttcaacct gccgtcctcg cacagggcgc gcctgagcgg attcggatta gctattccag
     4021 cggcgggatg acctccatcg atctcttcat cgcgcgcgat cgaaagtatt tccaacagca
     4081 gaatctaacc ggcgaattga ttcgaatgtc ggccaatctc gcgatcagtg ccggcatttc
     4141 cggagacgtt gatgttctcg gttccatcgg cagcgcgatc cgttcgattc agcgcggcgc
     4201 acctctacgg gttatttcgg taaccttgcg ccggccgttg ttctttctcg tcgcccgccc
     4261 ggagtacagc acggtcaagg atctaaaggg aaagacgtta ggcattgtca ccttcggcgg
     4321 cagtcagcac accactgcga aacgcttgat cgcgctgcac ggccttaatg ccgacaagga
     4381 gcttacttcg attcaggttg gcgaagaatc gatgcagctg caggctttga cgagcaatgc
     4441 gatccaggta gcggccatca gcccgcctta tgcgcaaatt gcccgcgaca aattcaacat
     4501 gaagatcctc gaaacgtcca ccgataaatt tgccagcatc cagaatggcg ccgccgtgaa
     4561 cctgaagatg ctccaggaga ggccggactt tttaaagcgc tttctgcgcg cccgcgctca
     4621 ggcgaataaa tattttttgg agcgagaaaa ggaagccagc gagcttctcg ccagcatctg
     4681 gaattccgat tttaaaatcg ccaacgaaag ttaccgggcc tccaaagcag cgttcaccgg
     4741 caccggcatt cccaccgatg acgagatcaa agagtatcta gcgctcgacg cgcaaatcat
     4801 cggcctgccg caaccggttc cggcggccag cgtcttcgac ttcaccttgc agcgagaaat
     4861 caataaagag ctggggatca agtaaatcga tttactcgaa tgctatcgcg gcgcgcgaga
     4921 ctctgggaac ctatcttcat tgtttgagcc gtgctggcag ttcattcttg aatcgcatgc
     4981 ccgcaaaatt ctgatctgcc tctaactcct gaagtttgcg gcgctgttca gtgctcaact
     5041 gcgcttttcc tttttcgata gaacgcacgc gcgcgatccg tagatcggcg cgaattcgct
     5101 cgatgtcgcg gatcttggcc tcgactcgtg tcatgtcaac cggttgcgcc tcaagcaagc
     5161 tggtcagatc caattcggcg atgcgaagat ttgcctcgct tcgaatcgac tctttggcaa
     5221 aatcattgcg cagttgctcc aagtgcttga cttgctctac cgttaaaccc agccgttcgc
     5281 gattgcgcag cattagtgaa ataggcgctc ggtcttccgc ccaaatctcg aaagcaaaat
     5341 tttctttcaa ccggtccagc agttcgcgca acttgcggcc gatactctcc ggcgtcaagc
     5401 ttcgatcctc tgagctcaaa ttcgattcat cggccgccgg gagtttaccg ggctcaacta
     5461 ttaagattaa cgtcagcagc aatcctaaac ttacgtagat cttcatggcg tctccttaca
     5521 cattcgacga tagttaaaat ctgagcttct atgcgctcga tgtcaaacct cccaagtcaa
     5581 tgtcaacgct cattctatta gcagtccaag gttccacaaa ataatttttg actgcaccaa
     5641 ttatcgcttg acaaataatc tcgtcagtaa cagattataa gtggatccgc tgagcgctgt
     5701 acggcgcggg gctaggtgga ttgccgatca aaagccccac gggaaaggga ggtctttgct
     5761 atgcaagacg gaactccacc gggtcatcaa agcccgctgc ttgcgggccg agtattctac
     5821 cggacgaaga agttctcgcg gtagagctgc tgtccgatcg cttcaacaaa ttgcctgaag
     5881 gcaaatagtc gcgtagcgac tgcagtaagc gctcatcggc aaaatgctct gtagcgagtg
     5941 tgtgtccgag gatgctccga gatgcgaaca gcgggcagtt aaaagagttt tgaacgttta
     6001 gcgtcaaaag cgcaaaaggc gcc
//
LOCUS       DASUTG010000665         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2179107, whole genome shotgun sequence.
ACCESSION   DASUTG010000665 DASUTG010000000
VERSION     DASUTG010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179107"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1126)
                     /gene="ruvB"
                     /locus_tag="VF089_15345"
     CDS             complement(53..1126)
                     /gene="ruvB"
                     /locus_tag="VF089_15345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX6175381.1"
                     /translation="MAHSPIIDPEKSSESREISPARSDEDLNYEVNLRPRGFDEYVGQ
                     DRVKENLKVAIDAARGRGEVLDHLLFHGPPGLGKTSLAYIIAREMGVNIKGTSGPVIE
                     RAGDLAAILTNLEEGDILFIDEIHRLNHIVEEVLYPAMEDFQIDIMIGQGPAARSIKL
                     ELKPFTLIGATTRSGLLTSPLRDRFGLSFRLDFYSPEILTDLLQRSAVILGVAGEHDG
                     IAEIARRSRGTPRIANRLLRRVRDYAQVKAAGRITLGVTVEALQMLEVDSIGLDKMDT
                     MLVLTLIEKFSGGPVGVETLAAAIGEEKDTIEDVYEPFLIQAGFIQRTPRGRVATPLA
                     YDHFGQKPKGQKGSGPKNQPTLF"
     gene            complement(1134..1727)
                     /gene="ruvA"
                     /locus_tag="VF089_15350"
     CDS             complement(1134..1727)
                     /gene="ruvA"
                     /locus_tag="VF089_15350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX6175382.1"
                     /translation="MIAQISGTLAQKVAGEIVVDVGGVGYQVFIPLNVFYHLPEIGAP
                     VRLHIHTHLREDALQLFGFQEPAEKQVFLQLNGVAGIGPKLALNILSGIPADELTRAI
                     KGGDQLRLVSIPGVGKKLAERMIVELRDRFALLESQASQTSVQGRDPQVIQDAVSALV
                     NLGYRKGEVEKNVRDCVEAGNHGLEQVIKEVLRRMSR"
     gene            complement(1770..2294)
                     /gene="ruvC"
                     /locus_tag="VF089_15355"
     CDS             complement(1770..2294)
                     /gene="ruvC"
                     /locus_tag="VF089_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX6175383.1"
                     /translation="MDPGSAVTGWGVVEAAGTSLVHLAHGTVVTAGIHGQAARLERIY
                     RGLEEVIESYHPDGVCVERVFFARNPQSALKLGQARGVAILAAARRSLAVHEYAAVEI
                     KAAVVGYGHATKQQVQMMIASLLQLSGKLPADAADALAAAICHLHMQVLRARLIEALP
                     KNIAPLRPRKSILS"
BASE COUNT          511 a          686 c          614 g          514 t
ORIGIN      
        1 tcaggcataa ggttcgattc cgcgcgtgct ggtggattca ggcgttgcta atttagaaca
       61 aagtgggttg gttcttaggg ccactgccct tttgtccctt aggtttttgc ccaaagtgat
      121 cgtaagccaa aggagtcgcc acccggcccc ttggcgttcg ctggataaag cccgcctgaa
      181 tcagaaacgg ctcgtagaca tcttcaatgg tatctttttc ctcgccaatg gccgctgcca
      241 gagtctcgac cccaacgggt cctccggaga acttctcgat cagggtcaat accagcatgg
      301 tatccatttt atcgaggcca atcgagtcga cctccaacat ctgtaacgcc tctacggtga
      361 ccccaagcgt gatccgtccc gctgctttga cttgcgcgta gtcgcgcaca cggcgcagca
      421 ggcggttggc gattcgtggc gtgccgcgcg agcgccgcgc gatctcggcg atgccgtcat
      481 gttcgccagc gaccccgaga ataacagctg atcgctggag aagatcagta aggatttccg
      541 gtgaataaaa atccaaccgg aagctcaagc cgaaacgatc ccgtagaggc gaggtgagca
      601 gacccgacct tgtcgttgcg ccaatcaatg tgaatggctt cagttcaagc ttgatggatc
      661 gcgccgccgg tccttgtccg atcatgatat caatttggaa atcttccatc gccggatata
      721 ggacttcttc gacaatatgg ttaaggcgat gaatctcgtc gataaacaag atatcgcctt
      781 cctccaaatt ggtgagaatc gccgccagat caccggcgcg ctcgattacc ggccccgagg
      841 tccctttgat attgacgccc atctcccgag cgatgatata ggccagtgac gtttttccta
      901 atcctggcgg gccgtgaaac aaaaggtggt ctaaaacctc gccacgaccg cgtgccgcgt
      961 cgatggctac tttaaggttt tctttgacac gatcttggcc gacgtattcg tcgaatccgc
     1021 ggggacgcag gttcacctcg tagttcagat cctcgtcgct tcgcgccggc gagatctccc
     1081 gcgattccga ggatttttca ggatcgataa tcggtgagtg ggccatgtcg gtgtcaacga
     1141 ctcatgcgcc ggagtacttc tttaatcacc tgctcgaggc cgtgattgcc tgcttcaaca
     1201 cagtcacgca cgttcttttc aacttcgcct ttacgataac ccagattaac gagagccgaa
     1261 acagcgtctt ggatcacctg cgggtcccgc ccttgaacag atgtttggct ggcttgagat
     1321 tcgagcagcg caaaccgatc acgaagctcg acaatcatgc gttcggcaag cttcttaccg
     1381 acgcctggga tcgataccaa gcgcaactga tcccctccct tgattgcgcg ggtgagttca
     1441 tcggcaggga ttcctgacaa aatgttcagc gcaagtttgg gtccgatgcc ggcaacaccg
     1501 ttgagttgca agaatacttg tttttccgcc ggctcctgga acccgaaaag ctgtaaggca
     1561 tcctcgcgca agtgagtgtg aatatgcagc cggaccggtg ccccgatctc cggcaaatga
     1621 taaaacacat tgagcgggat gaagacctga tagccaaccc caccgacgtc gacgacgatc
     1681 tctcctgcaa ctttctgcgc cagagtaccg ctgatttggg cgatcacgac tgactaagct
     1741 ttcggtcgat tgcaacgccg ttcggcattt caagaaagga tcgacttgcg cgggcgcaac
     1801 ggcgcgatgt ttttaggcag ggcttcgatg agacgcgcgc gcaatacctg catatggaga
     1861 tggcaaatag ccgccgctag cgcgtccgcg gcgtccgccg gcaacttgcc gctaagttgc
     1921 agcaaagacg caatcatcat ttgcacttgc tgtttggttg cgtggccgta gcctaccacc
     1981 gcagccttga tctcaactgc ggcatattca tgaactgcca aactccttcg tgcagccgca
     2041 agaatggcta cgccccgcgc ttggccaagc ttgagcgcgc tctgcgggtt gcgcgcaaaa
     2101 aaaacccgct cgacgcaaac accatccggg tgataggatt caatcacttc ttcaaggccg
     2161 cggtaaatcc gctccagccg agccgcctgc ccgtgaatac ccgccgtaac caccgtgcca
     2221 tgcgccagat ggaccaatga tgtgcctgcc gcttcaacca ctccccaacc ggttactgcc
     2281 gaaccgggat cgataccaag aaccctgcgc gcaagcggcg cggca
//
LOCUS       DASUTG010000666         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1883503, whole genome shotgun sequence.
ACCESSION   DASUTG010000666 DASUTG010000000
VERSION     DASUTG010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883503"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VF089_15360"
     CDS             <1..986
                     /locus_tag="VF089_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175384.1"
                     /translation="TVSTNAEATNFQVGDYLLVHIIVWKPAQANEKADAQRQLWGLFK
                     AKHKNATDVEWEPQEDSADKNVFNSRIFGSKRVAILLINFMTPSTWDVKYTVGLDRRI
                     PTPIQNVLTLAANVGAVGGAAEATAAPLAPKNIWGARMMLVRYSASDMVVKVNSITNN
                     NAEIEQARDYSKKYLNEGRYHWDVSIGLPVKSIKELQYKTDANNRVTTEAKERQDAYG
                     FLNLFPKAVDLSGERFLQVPHFVFGVPLASKPLHRPFAGIGYGVYKTPIKFNFFGGIV
                     FNRQRVPRTINVGNSANADQLDSDLRTRWVRKFMFGVNFPISQIKDAIKNK"
     gene            1587..2168
                     /locus_tag="VF089_15365"
     CDS             1587..2168
                     /locus_tag="VF089_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175385.1"
                     /translation="MMGLNISKPPAGARAIVSERLGALTTHGAPASEMLAETSSEQLD
                     LAAPHPVYFIKLTDVAEGRLLSAAILTGWRYLVLQGEEAVGAATVAVLENNESLRFSH
                     MSQGPFVKNTIEGIRHAEELPEVLENDYELRMLDIPSLYVVALWLHGQEDRIIPLAPT
                     NRALDPYKTYSEDELLSALKEAALNRLEFEEKA"
     gene            2581..3279
                     /locus_tag="VF089_15370"
     CDS             2581..3279
                     /locus_tag="VF089_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX6175386.1"
                     /translation="MATGNDLLTIAAPHVGEEYILGALAPKNNANWGGPWDCAEFVSW
                     CVFQAGGMLYGCHSNSAPPASADAYTGYWRRDAGALGKKISIDEAARTPGAALLRVPS
                     PGANGHIVLCDGHGGTIEAMSPSQRVRRHVVTGRRWDMGILVPGITYEVNQGGNIGEP
                     PTVYRLTSPFMRGEKVREIQQALKDAGVDPGSIDGIFGPKTKAAALGFQLAKGLVADG
                     EVGPETAGALGIEL"
     gene            complement(3316..3948)
                     /locus_tag="VF089_15375"
     CDS             complement(3316..3948)
                     /locus_tag="VF089_15375"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX6175387.1"
                     /translation="MIRTIEPEENEVPARREHTWASRAGWVEVIAGSMFSGKSEELIR
                     RLRRAKIARQKVQVFKPEIDSRFSESHIVSHSEMRHESSNVHTAAEIRALVQPETEVV
                     GIDEAQFFDNELIAVANELAGRGMRVIVAGLDTDYTAKPWEPMPQMLAIAEYITKTHA
                     ICMRCGQPANYTQRTFESEERVAVGGAGMYEARCRACFVPHADAPTVHEG"
     gene            complement(4222..4335)
                     /locus_tag="VF089_15380"
     CDS             complement(4222..4335)
                     /locus_tag="VF089_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6175388.1"
                     /translation="MIRRDGRREELTAKGNWQLEAGDRVRIETPGGGGYGK"
BASE COUNT         1045 a         1153 c         1167 g          984 t
ORIGIN      
        1 taacggtatc gaccaacgcc gaggctacga actttcaggt tggggattac ctgctggttc
       61 acatcatcgt ctggaaaccc gctcaggcga acgaaaaggc ggatgcgcaa cgccaactct
      121 ggggactctt caaagccaag cataagaatg cgactgatgt tgagtgggag ccacaggaag
      181 attcagcgga taagaacgtc ttcaattccc ggatctttgg gagtaagaga gtcgcaatac
      241 ttttgatcaa cttcatgact ccatcaacct gggacgttaa atacacagtg gggctggatc
      301 gaagaatacc gactccaatt cagaatgtgc taactctggc ggctaacgtg ggtgccgtcg
      361 ggggcgcagc ggaagccacg gccgcgccac ttgctccaaa aaatatctgg ggtgcacgta
      421 tgatgctcgt aaggtattcg gcttcagaca tggtagtgaa ggtaaacagc atcaccaaca
      481 acaatgctga gattgagcag gccagggact attccaagaa atatctcaac gagggtcgct
      541 atcactggga tgtgagtata ggcctgccgg tgaagagcat taaggagttg caatataaaa
      601 ctgatgccaa caaccgcgtg accaccgaag ctaaggaaag gcaggatgcg tatggctttc
      661 tgaacctttt cccaaaagca gtggaccttt cgggtgagcg ttttctccaa gtgcctcatt
      721 ttgtatttgg ggttcccctt gcgtcgaaac ctttgcaccg tccattcgcc ggaattgggt
      781 acggagttta caagactccc attaagttca actttttcgg agggattgtg ttcaaccggc
      841 aacgcgttcc acgaacaatc aacgtgggca actcagctaa tgccgatcaa ctggattcgg
      901 atctgcgcac ccggtgggta agaaagttca tgtttggggt caattttcca attagtcaga
      961 ttaaggacgc aatcaaaaac aaatgaggag cggaaagatg gatctgccga cactcgtgaa
     1021 atccagcaga cagaagctcg atccagcggc gatctcaccc gccttagctt ccggtccagc
     1081 tgactcggga actctgcttt attggcagga gactcaggag caagatcaat ggtgctgggc
     1141 agccgttacc gttagcgtct caaagtttta cgatcctgcc agcagttgga cgcagtgctc
     1201 cttggttaat gccgaattga acagggatga ctgctgctgg gacggagggt cttcaggttg
     1261 caatcgaccg tggacacttg aggccccact ttccgaaacc aataatctca attggacgga
     1321 tggctcagct gcagtttttc ccgatatcgc cgctgagata aataaccgcc ggctactcgg
     1381 ctgccgaatc ggttgggcgt cgggtggggg ccatttcgta gttattcatg gcttctcgga
     1441 tgggcccaac ggttcttggg tttcagtggc agatcctttt tacggatcgt ccacatatgc
     1501 ctacgatgtc ttttgcacca attaccgtag caacggagaa tggacgcaca gttacttcac
     1561 tcaaccatga cgtgaaagga cgttggatga tgggtctaaa tatttcaaag ccgccggcag
     1621 gtgcgcgggc aatcgtcagc gagcgccttg gagcgttgac cactcacgga gctccagcct
     1681 ccgaaatgct ggcagagaca tcgtccgaac aacttgatct cgcggcaccg catcccgtct
     1741 attttatcaa gcttacggat gtggccgaag gaaggctact ctccgccgcg attctcactg
     1801 gctggcgcta tttggtgttg caaggtgagg aagcggtcgg cgccgctact gtcgcagtgc
     1861 tggaaaacaa tgaaagtcta agattttcac acatgagtca gggacctttc gtaaaaaaca
     1921 ccatagaggg aatccgtcat gcagaagagc tcccagaggt tttggaaaac gactacgaac
     1981 ttcgcatgct ggatatcccc agcctttacg tagtagcgct gtggctgcat ggacaggaag
     2041 atcgaattat cccactagcg cctacgaatc gcgcgctcga tccttacaaa acctattccg
     2101 aagatgaatt gctttcagct ttgaaagaag ccgctcttaa tcggttggaa tttgaggaaa
     2161 aggcatgatc ttgtcaccgg cttccggatg ttctccgccc atttaagaaa atttaatcct
     2221 catgcaaccg tggcgcgtcc gcgtgtggca cgaagcaggc accccagtgt gcttcgcaca
     2281 tgccgccgcc gacggcgaat caccccttta gattctaaag accgccgagt gtagttcgcg
     2341 ggttgctccc caacgtcagc atttggcgtg cgtcctcgta atgtactcag caatcgcgcg
     2401 gctcctaagg cttctccatt caaacacaag tgcttctcca ttcaaacaca agttaggcat
     2461 ccagtaacct catcccatgg aaatcccgcg ccttgaaatg tttcgctgaa aatcctcatc
     2521 cacattaaag ggaacgccgc cgcgagagtc ggccggcgat aaagcgaaag gaaaaaaatc
     2581 atggctaccg gaaatgacct gttaacaatc gcggcaccac acgtcggaga ggagtacatc
     2641 ctcggcgcac tggctcccaa aaacaatgca aattggggag gcccgtggga ctgcgccgag
     2701 tttgtttcgt ggtgcgtttt tcaggcaggc ggaatgctgt atgggtgtca ctcgaacagc
     2761 gcaccgcccg cgagtgcaga cgcttacacg ggatactgga gaagggacgc cggcgctctg
     2821 gggaagaaaa tcagtatcga cgaggcagca cgaaccccgg gtgccgctct cctcagagtt
     2881 ccaagcccag gcgcgaatgg acacattgtc ctctgcgacg gacacggtgg gacgattgaa
     2941 gccatgtcac ccagccagcg agtaaggcgg cacgtggtca ccggcagacg atgggacatg
     3001 ggcattctgg ttccgggcat tacgtatgaa gtcaatcaag gcggcaacat cggtgaaccc
     3061 cctaccgtct accggcttac ctcgcccttc atgagaggcg agaaggttcg ggagattcaa
     3121 caagcgctaa aggatgccgg agtggacccc ggatccatag atggtatttt cggcccaaag
     3181 acaaaggcag cggcgcttgg atttcaactc gcaaaaggtt tggtggccga cggcgaggtt
     3241 ggcccggaaa cggcgggcgc tctcggaatc gagttgtagg tcccagacta cgaaaacgcc
     3301 gggaagactt ttccctcacc cctcgtgcac tgtcggggcg tcggcatgcg gtacgaaaca
     3361 agcgcgacag cgtgcttcgt acatgccggc gccgccgacg gcgacgcgct cttcagattc
     3421 gaaagttctt tgcgtgtagt tcgccggctg gccgcacctc atgcagatcg cgtgggtctt
     3481 ggtaatgtat tcggcaatcg cgagcatttg cggcatcggc tcccacggct ttgcggtgta
     3541 atcggtatca agtccggcca cgatcacgcg catcccacgt cccgccaatt cattagccac
     3601 cgctatcaac tcattgtcga agaactgagc ttcatcgatt ccgacaactt cagtttccgg
     3661 ctgcaccagc gcgcgaatct cggccgccgt atgcacgttc gaagactcgt gacgcatctc
     3721 tgagtgcgag acgatgtggc tttccgaaaa gcgcgagtcg atttcaggct tgaacacctg
     3781 cactttttgc cgcgcgattt tggcgcgccg cagacgacga atcagttcct ctgacttgcc
     3841 cgaaaacatc gagccggcaa tcacctcaac ccaacctgcg cgagacgccc atgtatgttc
     3901 tcttctggcg ggtacttcgt tttcttcggg ttcaatagtg cgaatcattg ctgctgaagg
     3961 attcattatc atcgttatct gaatgaactt gtctcgaaaa ccgacgcagt ctagcacagt
     4021 ccacagatta cacagattac acagataaga cttgagaaag ctaagttggg aaaggcttcc
     4081 ctttggcctg aagcttggtt tttcttgcaa acgatttggt atgcctcgta gttttgcgcg
     4141 agaaatgcgt cacaatggga aagaatgatc tcactactta tcggtccaag tcttatctgt
     4201 gtaatctgcg taatctgtgg attactttcc gtatccgccc ccgcccgggg tttcgatgcg
     4261 cacgcgatcg cccgcttcaa gttgccaatt tcctttcgca gttaattctt cacggcggcc
     4321 gtcgcgtcga atgataaaag cgcgcccac
//
LOCUS       DASUTG010000667         7390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2179644, whole genome shotgun sequence.
ACCESSION   DASUTG010000667 DASUTG010000000
VERSION     DASUTG010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179644"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF089_15385"
     CDS             complement(<1..868)
                     /locus_tag="VF089_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6175389.1"
                     /translation="MYNKILVPLDGSKAAEAVLPHVEIFARALQLPVDLVHVTDTETV
                     PPSIHTGHQADYLKQLAASCLNCLRVNCSVKSGSAAEVILDAASADPGILIAMATHGQ
                     SAGARWLLGRTSQKVLQASKNPLLLIRSDRPNGPVREAHLKTIIIPLDGSALAEQIFP
                     HVVYLATKLGLNVVLIRTYTLPTASYFMGAHVSPPDRAELAEKIKKEALDYLQAKVEE
                     LRPAGVEKISFVLAEGKGPEEIIDLSQRTSDNMVAMSTHGRSGIGRWVLGSVADRVIS
                     YCGDPVLVIRPSQ"
     gene            1161..1934
                     /locus_tag="VF089_15390"
     CDS             1161..1934
                     /locus_tag="VF089_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX6175390.1"
                     /translation="MITDPAGIEFLQWCLPRLHLRWQGFRKVRRQVYKRISGRLQALG
                     LPSLVAYRDYLENHSGEWTTLDSLCWVSISRFYRDQSAFQLLERDILPRLAELALGAG
                     EGATLSCWSVGCAGGEEPYTLAIIWHQRLASQFPSLHLRVVATDIDSQAIRRAERGCY
                     QASSLKQLPPEWRAQAFMTIGDELCINQEYRGAVTFALQDIRERAPEGVFHLILCRNL
                     VFTYFDETLQREILERLKLSLVPGGALMIGNLESLPESV"
     gene            complement(2042..3091)
                     /gene="rtcA"
                     /locus_tag="VF089_15395"
     CDS             complement(2042..3091)
                     /gene="rtcA"
                     /locus_tag="VF089_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX6175391.1"
                     /translation="MLEIDGSQYSGSGAIVRQAVAFAALTGTAIHIYNVRFKRPKPGL
                     RLQHAQAVEAIRQLVNGTTEGAVAGAREFIFHRGKPTGSQKFSWDIGSAGSTTALALA
                     VLPVLAFSSFPLSIELRGGLFQDFAPSFYHLEHVLLPLLYRMGVEASAQMERPGYVPT
                     GGGIWRLAVAPTRGTLRQLLLDTPGIVAKVWGIALSSRLKEQLVSDRMAESAKRAFEA
                     AGYHADVEVIYDTSALQAGAALAIFADLSSGARIGSDRAGAPGRRSETIGTQVARRLL
                     EDLKTGATLDRYASDQIIPFAALAAGESRFHIPGVSKHIESSAWLSREFLGADVRADG
                     HELVVEGVGFRARAT"
     gene            complement(3101..3403)
                     /locus_tag="VF089_15400"
     CDS             complement(3101..3403)
                     /locus_tag="VF089_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX6175392.1"
                     /translation="MGTYVMLTRLSPEALKNPESVTRLNKQVEDRIKKDCPGVKWLSN
                     YAVLGPCDYLDIFEAPDPDTATKVALLIRSFGHGTTETWFATPWDRFTELATALKS"
     gene            complement(3446..3568)
                     /locus_tag="VF089_15405"
     CDS             complement(3446..3568)
                     /locus_tag="VF089_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6175393.1"
                     /translation="MTKPAICVHEEEDVEGVIAKMTDENISNLRVTAEDGRQEH"
     gene            4136..4468
                     /locus_tag="VF089_15410"
     CDS             4136..4468
                     /locus_tag="VF089_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175394.1"
                     /translation="MYVSRLTFHTLPGKTHEVEEGLLTLLKWVENAGGRNARVMRTHF
                     GSLGAPDLVFEQEVDDPGTLERQIKTVTEGLDFQPWAKKISGLLEESSKRELYQVTAP
                     SVIRAGES"
     gene            4704..5126
                     /locus_tag="VF089_15415"
     CDS             4704..5126
                     /locus_tag="VF089_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX6175395.1"
                     /translation="MAKAGYELTPHGADIGVRGVGSSKEECFEQAAVALTAVITDPNT
                     VAAKELVEVTCEAPDDELLLVEWLNALVYEMATRTMLFARFEVHIDGHKLTGKAWGES
                     IDANRHELAVEVKGATCTELKVAQDAEGAWLAQCVVDV"
     gene            5136..6566
                     /locus_tag="VF089_15420"
     CDS             5136..6566
                     /locus_tag="VF089_15420"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020146856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6175396.1"
                     /translation="MDTTRLQRQTEHQWQIAPHGKMRVPAVIFATESLIRDMDEKVYE
                     QVTNVATLPGIVRASYAMPDAHWGYGFPIGGVAAFDPDNGGVISAGGVGFDISCGVRT
                     LLTGLTLEQIQAVKERLADALFHKIPAGVGSRGKLSLNEKEMDSMLLGGAQWAVKQRY
                     GTVEDLQRVEESGCIADARPTEVSDRAKKRQRDEMGTLGSGNHYLEVQHVAKIIDRGI
                     ASAFKVFEGDVVLSIHCGSRGLGHQIGTEFLTRMAAAASEYGIDLPDRELACAPIYST
                     VGRAYLGAMRGAINCALANRQIITHLVREAFTEIFPQAELPLLYDVSHNTCKVEDHIV
                     DGREMHLFVHRKGATRAFGPGHHAVAEPLRAIGQPVLIGGTMGTASYILSGTTAGMDL
                     AFGSACHGAGRSMSRHHACRQWRGSEVVQNLASRGVLIRSRSMRGIAEEAPGAYKDVT
                     EVVEAADHAGLARTVARLEPVICIKG"
     gene            6585..>7390
                     /locus_tag="VF089_15425"
     CDS             6585..>7390
                     /locus_tag="VF089_15425"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX6175397.1"
                     /translation="MSLEIVTGSSNYALAEEVAKNLGVKLVPRILERFPDGELNIEIK
                     QSVRGCDVYLIEPTCPPVDEHFFELFLLADACRRAGATHLTAVIPYFGYARQDRRAHG
                     REPVSLRLLADLIAASAVQRVVVVDFHSRAVESAFAVPVEHLSAISILANAVRPLVHK
                     DDVVVSPDLGAVKMAEHYARLLGLSVAIIHKTRISGADVTVQRIIGDVRDREVVVVDD
                     MITTGGTIEKAIKALLDAGCSSSGIKIVASHGLFVGNAADRLAQLPIEKI"
BASE COUNT         1672 a         1995 c         2087 g         1636 t
ORIGIN      
        1 cctgagacgg tcggatcacc aacacgggat cgccacaata agaaattacg cggtccgcaa
       61 cgctgccgag aacccagcga ccgattcctg atcgtccgtg cgtggacatc gcgaccatgt
      121 tgtccgaggt tctttgcgac aagtcgatga tctcttcggg tcccttccct tcagctaaga
      181 caaacgatat tttttctact cctgcgggcc gtagttcttc gactttcgcc tgcagatagt
      241 ccaaagcctc tttctttatt ttttctgcga gctcggctcg gtccggaggg gagacgtggg
      301 ctcccataaa ataactcgcc gttggaagag tgtaggttcg gatcagaacg acgttcaatc
      361 ctaactttgt cgcaaggtaa acaacatggg gaaagatctg ctcggccagg gccgagccat
      421 cgagcggaat gatgatagtc tttaagtgcg cttcacgaac ggggccgttc ggcctatcgg
      481 agcgaatcag cagaagagga ttctttgatg cctggagcac cttctggctt gtacgcccca
      541 gcaaccagcg cgcgcccgcc gattgtccat gcgtggccat ggcgataagg atgccgggat
      601 ccgctgaagc ggcatcgagg atcacttcag ccgcactgcc gctttttacg gagcaattca
      661 cccgcaaaca gttcaagcac gaagctgcca attgcttcag ataatcagct tgatgccctg
      721 tatgtatcga gggaggtacg gtttccgtat cggtgacatg gaccaaatcc acaggcagct
      781 gcagcgcacg ggcaaaaatt tcgacgtgtg gcagaactgc ttcagccgcc ttggatccat
      841 cgagtgggac aagaatcttg ttgtacatat aactcgcttt ctcgtgtgag cgtcatcccg
      901 ctcccgtcag gtcgatttgt acgatgacag taaaaagaca attgcctacg taagcgagca
      961 attcacatgc ctttggttta ccggattact agccgcttct aatcgcagtc ctggcaattg
     1021 acggcactct tcctcgcaga aatgcaaaat ctcgccgcga cgggcaaagg gagtttgatg
     1081 tctccatcag acacccctcg ctacggccgc aacgagagga ttggtgtacg agttgcgtcc
     1141 tctagatcaa ggacatctgc atgattaccg atccggcggg catcgaattt ttgcagtggt
     1201 gtttgcccag attgcatctg cgctggcagg ggtttcgaaa agtgcgccgg caagtctata
     1261 agcggatcag cggacggctg caggcgttag gattgccaag tcttgttgcc tatagagact
     1321 acctcgagaa tcattccggc gagtggacga cgctcgatag cttgtgttgg gtttccattt
     1381 cgcgattcta cagagatcag agtgcctttc agctgttgga acgtgacatc ctgccacgac
     1441 tggcggagtt ggcgcttggc gcgggcgaag gcgctacatt gagctgctgg agcgtcggct
     1501 gtgccggagg tgaagaaccg tatacgcttg cgataatctg gcatcagcgt ttggcgtccc
     1561 agtttccgag cctgcattta cgagtcgtgg caaccgatat cgactcccag gcaattcgac
     1621 gggccgagcg gggctgttat caggcgagca gcttgaagca actgccgccc gaatggcgag
     1681 ctcaggcgtt catgacaata ggcgacgaac tctgcatcaa ccaagagtat cgaggcgccg
     1741 tgacattcgc attgcaggat atacgcgagc gcgcacccga aggggtgttt cacctcatcc
     1801 tctgccgtaa tcttgttttt acttacttcg acgaaacatt gcagagagaa atcctggaga
     1861 gacttaagct gagcttggtt ccgggcggcg ctttgatgat cggcaatctc gaatcgcttc
     1921 ccgaaagtgt ctgaggattc acgccgtggt cgaatcgatc gggagcgtac cgaaaagtct
     1981 agctaacgat tattcagcga caagcgcaca gcgtccggca aaaggggaag ccattatcta
     2041 tctaagttgc tctcgctcta aaaccaacgc cttccactac caattcgtgt ccatcagccc
     2101 tgacatcagc gcccagaaac tcgcggctca accacgcgct gctctcgata tgcttggata
     2161 ctccagggat gtgaaatcga ctttcgccgg cggccaacgc ggcaaaagga atgatctggt
     2221 cgctggcgta acgatcgaga gtcgcaccgg tttttaaatc ttccaagagc cggcgcgcga
     2281 cctgtgttcc gatagtttcg gagcgccggc ccggcgcgcc tgctcggtcg gagccaattc
     2341 gcgcaccgct gcttaggtca gcaaagatcg ccagggcagc tccggcctgg agcgcgctgg
     2401 tgtcgtagat cacctcaaca tcggcgtgat atccagccgc ttcaaaagcg cgttttgccg
     2461 attccgccat gcgatcgctg accaattgtt ctttgagccg tgacgaaagc gcaatgcccc
     2521 aaactttcgc gacgatcccc ggagtgtcca ggagcaactg tcggagagtg cctcgtgttg
     2581 gtgctactgc gagacgccag attccgccac ccgtcggaac ataaccgggc cgctccattt
     2641 gcgcgctcgc ttcgacaccc atccgataga gcagcggcaa taacacatgt tcgaggtgat
     2701 aaaacgacgg ggcaaaatct tgaaagagac cgccgcgcaa ttcgatcgat agcggaaacg
     2761 agctaaatgc caacaccggt agcaccgcca gcgcaagtgc cgtggttgat ccagccgagc
     2821 cgatatccca tgaaaatttc tggcttccgg ttggcttgcc tcgatgaaaa ataaattccc
     2881 gtgccccggc aactgcgcct tcggtggttc cgttcaccag ctgccgaatg gcttcgacag
     2941 cctgagcgtg ctgcaggcgc agtcccggtt tgggtctttt aaagcgaacg ttgtagatgt
     3001 ggatcgccgt tccggtcagg gccgcgaacg ctaccgcctg gcggacgatg gcaccgctgc
     3061 cggaatactg cgagccatcg atttcaagca cgctaagttg ttaactcttg agcgctgtcg
     3121 ccagttcggt gaagcggtcc cacggcgtgg cgaaccatgt ctcggttgtt ccgtgaccaa
     3181 atgaccgaat tagcaatgcc accttagtgg cggtatccgg atctggcgcc tcgaatatat
     3241 cgagataatc gcaaggcccg aggacagcgt agttggagag ccatttcact ccggggcaat
     3301 cctttttgat ccgatcttcc acttgcttat tcagtcgggt taccgactcc gggttcttta
     3361 acgcctcggg cgaaagtcga gtcaacatca cataagttcc catgcgaatt ctcctttctg
     3421 ggattcccaa caatatgacc gcaaatcaat gctcttgccg cccgtcttcc gctgtcactc
     3481 gaagattact gatattctcg tcggtcattt tggctatgac tccttcgacg tcctcttctt
     3541 catgcacaca gatcgccggt ttagtcattt atctcgtccg ccatagtcgt ttgcaggtct
     3601 ttgccctggc gtacagcatt caaaaaatcg aactcgtccc gacgccaaca accttgaagt
     3661 ggaatcgacc accagtagtc cgctgtacat gccggaaaga agctgcagag aaggatcgcg
     3721 atctaccgcg cttggggttg ccgcagcacc cgagtattca taacgtcagt gccttcatga
     3781 accatacttc cttgaacggt ggtgatatat ggatggacca gttggctaga cctgtgccgc
     3841 aacttcaagc ggcgattcca cgaaatgctg ttaccgacag aggtagaatt ttcattcttg
     3901 aaaaataaac cgtgacgaac cggaaaactc gagtccagtt tgatcggtaa atgcttgatt
     3961 acacgggaga ccactggtgt atcaatatgc gaaagaagta ctgctcggct tcgcgaatat
     4021 gagaagaaag tgacgattca gctaaagatt gcagcgagtc atcagcttcg gcaccttggc
     4081 attttccttg cgcaatgatt tcggcattgg atagcggaaa caggagggac gagctatgta
     4141 tgtatcgcgg ctgacatttc atacgttgcc cgggaaaacc cacgaagttg aagagggact
     4201 gctgacgctt ctcaaatggg tagaaaatgc gggcgggcgg aacgcccgag ttatgcgcac
     4261 acacttcggt tctttgggcg ctccggatct ggtctttgag caggaagtag acgatcccgg
     4321 aactctggag cggcaaatta agacagtcac agaaggactc gactttcagc cgtgggcgaa
     4381 aaagatttcc ggtctgttgg aggaatcatc aaagcgagag ctttaccaag tgaccgcccc
     4441 aagcgttatt agagccggcg agtcttgatt gcgcgagcaa taaccctggt caagccgttc
     4501 atttggagag aatgactggt taaccggagg aaagaattct ccatatgata agtagcaagc
     4561 cgttctgacg accgaccctg acgcccgagc aaggtttgcc ggacccaagc actaagcagc
     4621 gaagcatcga gggctcaagc ggcttacaag tgacgcctta ctgcttgcct cgagaatgag
     4681 ccgcagcgtt cgatggaagg taaatggcta aagccggtta tgagctcacc cctcacggtg
     4741 ccgacatcgg cgtaagaggc gtaggaagct ccaaggagga gtgtttcgaa caggctgctg
     4801 tcgctttgac ggcagttatc accgacccca acaccgtcgc agccaaagag cttgtggaag
     4861 ttacatgcga agcacctgac gatgagcttc ttctcgtcga gtggctcaat gctttggttt
     4921 acgagatggc gacccgaacc atgctgttcg cgcgatttga ggttcacatc gacggacata
     4981 aattgacggg caaagcctgg ggtgaatcta tcgacgccaa tcgacatgag ttggccgttg
     5041 aggtcaaagg cgccacgtgc acggaattaa aggtggcgca agacgcagaa ggcgcctggc
     5101 tggcgcagtg cgttgttgac gtctaggaga gtttcgtgga taccacgcgg ttgcagagac
     5161 aaacggaaca ccaatggcaa atcgctcctc acggcaaaat gcgagtgccg gcggtgattt
     5221 tcgctaccga gtcgttgatt cgcgacatgg atgagaaggt ttacgaacag gtgaccaatg
     5281 tcgcgactct gccggggatc gtcagagctt cgtatgccat gcccgatgcc cattggggat
     5341 atggctttcc aatcggcggg gtagcggctt ttgacccgga caatggcggc gtcatttccg
     5401 ccggcggcgt cggctttgat atttcctgcg gcgtgcgcac cctcttgacc gggctgaccc
     5461 tcgaacaaat tcaagcggtg aaggaaaggt tagccgatgc gctcttccac aaaattcccg
     5521 caggcgtcgg cagtcgaggc aagcttagtt tgaacgaaaa ggaaatggac tcgatgctct
     5581 tgggcggcgc gcaatgggcg gtaaagcagc gttacggaac ggttgaagat ctacaaaggg
     5641 tcgaggaaag cggctgcatt gcagatgcgc gcccaaccga agtttccgat cgcgccaaga
     5701 agcgccagcg cgatgaaatg ggaactctcg gatcggggaa tcattatctc gaagtccagc
     5761 atgtcgccaa gatcattgac cgcggaattg cttcagcgtt taaagtattc gaaggcgacg
     5821 tcgttctcag tattcactgc ggctcgcgcg gtctcggcca tcaaatcggc acggaatttc
     5881 tgacacgcat ggccgccgcc gcatccgagt atggaatcga tttaccggac cgcgagctcg
     5941 cttgcgcgcc gatctactca acggtcggga gagcttatct gggcgctatg cgaggcgcca
     6001 tcaactgcgc gctcgccaac cgccagatca tcacgcatct tgtgcgcgag gcgttcacgg
     6061 aaatttttcc gcaagcggag ctgccgctgc tctacgacgt gtcgcacaac acctgtaagg
     6121 tcgaagatca tattgttgat ggaagagaga tgcatctctt cgtccatcgc aagggcgcga
     6181 cacgcgcctt tggcccggga catcacgcgg ttgccgagcc actgcgagcg attggacaac
     6241 ccgtgctgat tggcggaacc atggggacgg catcttatat attgtcaggg acgacggcag
     6301 gcatggatct cgccttcggc tccgcctgcc acggcgctgg ccgcagcatg agtcggcatc
     6361 acgcatgtcg acagtggcgc ggctctgagg tcgttcaaaa tcttgcaagc cgcggcgtct
     6421 tgattcgtag ccgttcgatg cgcggcatcg ctgaagaagc gcccggcgcg tataaagacg
     6481 tgacagaagt ggttgaagcg gcggatcatg ctggcttggc gcgcaccgtc gcgcggctcg
     6541 aaccggtaat ctgcatcaaa ggctagtggc tttaggagag aaccatgagc cttgagatcg
     6601 tcacaggttc gtcaaactat gctcttgcgg aagaggttgc aaaaaatctt ggggtcaagc
     6661 ttgtgccgcg gattttggag cgttttcccg acggtgagtt aaacattgaa atcaaacaga
     6721 gcgtgcgcgg gtgtgacgtt tatctgattg agccgacctg tcccccagtg gacgagcact
     6781 tctttgaact gtttcttctg gccgatgcat gccgccgcgc aggagcgact cacctgacag
     6841 ccgtcatccc ttatttcggc tacgcccggc aggaccgccg cgcccacggg cgcgagcccg
     6901 tgagcttgcg gctgcttgcc gatctgatcg ctgccagcgc agtccagcga gtcgtggtcg
     6961 tggattttca tagccgcgca gtcgaaagcg cctttgcggt tccggtagaa catctaagcg
     7021 ccatctcaat tttagctaac gccgtccgac ctttagtcca caaagatgac gttgttgtct
     7081 cgccggacct tggcgccgtc aagatggccg agcactacgc caggttactc ggtttatcgg
     7141 tggcgattat tcataaaact cgcatcagcg gggccgacgt cacggtccaa cgcatcatcg
     7201 gcgatgtccg cgacagagaa gtagttgtcg ttgatgacat gatcacaact ggcggaacca
     7261 tagagaaggc gattaaagcg ctgctcgacg ccggctgctc atcctccggt attaagatcg
     7321 tggcgagcca cggtttgttc gtaggtaacg ccgccgatcg gctggcacaa ctgccgatcg
     7381 agaagattta
//
LOCUS       DASUTG010000668         6832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2179970, whole genome shotgun sequence.
ACCESSION   DASUTG010000668 DASUTG010000000
VERSION     DASUTG010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179970"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF089_15430"
     CDS             complement(<1..205)
                     /locus_tag="VF089_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175398.1"
                     /translation="MIENPFYGFGRIYRALSFHVKRSTMPVRTNLKDPAVVDKTKTIH
                     EKIALPRSWALGLPDSTVTARTTD"
     gene            206..1765
                     /locus_tag="VF089_15435"
     CDS             206..1765
                     /locus_tag="VF089_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX6175399.1"
                     /translation="MPQPPFNPAWFVRKDLDGFFGLMIDNLIQLILIVSLCRELIQLP
                     DEYIFGRILPGAAVSILVGNLFYAWQARRLAREVGREDVTALPYGINTVSLFAFIFFI
                     MMPIYQETQDPIWAWKVGLVACFLNGAIEIAGAFVADRVRSVTPRAALLSALAGIAIT
                     FISMDFTFRIFAQPLVALIPLALIFIAYFSQQRFPLGLPGGMVAVAVGTLLGWWLGAV
                     KGPASVNFALARPDFTGGSLLEALLHPAFLNYLSVIIPMGIFNVVGSLQNIESAEAAG
                     DRYPAFPSLLANGVGSVVAALLGSVFPTTIYIGHPGWKRLGARSGYSVLNGLFIASIC
                     LTGTIQAVLGFIPLESGIGILLYIGVVIVAQSFQETPKEHAPAVALGIVPALAAWGLL
                     MVETGLRVAGKSLHSIGLAPFGQNPAMHGMISLQQGFIFTSMILAAIGVALIERQFKK
                     AAAWSLVAAVFSAIGLIHAYELTPAGVAARFGLLVAPEFAASYLLLALLFFAFAFAPG
                     KRHKNAKPGSP"
     gene            complement(1790..2407)
                     /locus_tag="VF089_15440"
     CDS             complement(1790..2407)
                     /locus_tag="VF089_15440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6175400.1"
                     /translation="MQIVLLRHGATDWNLQGRCQGSSDRRLTEVGLRQAEQIAIQLGN
                     ETIHAIYSSSLERARQTAALVSRPHNLPVMIEDDVRELDHGQLEGLTFNEIKAKYSDF
                     LTIWRTAPAELQIPGGERLADVAERAWRALSRIAQRHQTDQTVVVVSHNFPILGVVCR
                     VTGTDLNNYRSFHLDPCGVTRITQHRNGLWEVTHVNSKPYTLPRP"
     gene            complement(2424..4334)
                     /locus_tag="VF089_15445"
     CDS             complement(2424..4334)
                     /locus_tag="VF089_15445"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX6175401.1"
                     /translation="MLDFLRKKKRSWIITILLGLIIVVFVAFYGGSQQPTGVASDVAE
                     VNGESISQREFVMHYQRAVERYREMFKGSLTPELLKNLNIKHSLLEEMIEARLVLQEA
                     RRLGLAATDEELMNSIAQIPEFHVNGRFNKERYIQLLRANRLTPAQFEEDQRKQLTIQ
                     RLLGVLADAAHVTEAEVRERYRFEQEKINLQFVRFSTSDYLSEVQVTDEEIQKFYERN
                     KESLKEPLKVQVEYIPYPFDQFSTPVQLTDKEVEDYYNANRVTKFTTPKQAKVRYIMV
                     RLDPGADAKQKETALARANRIVAEARGGKNFAELARKESGDPSAEKGGEIGWLNQGQL
                     PEALDQQIFALAKGEISEPIETPLGFHIVKIEDVKEARTQSLAEARPVITRELQLEKG
                     KYEAAKSADRDREKAASGNDLSKLAQESGLSLRTTRLFSEGEVLPEIGPTQDFYKTAL
                     SLRANELSSVIEGPNAFYILKVKERKEAFVPPLDTVRGKIEKGLKESKAYEIAVQKAN
                     DLLDQFKKEPNLAKLARDKKLKLEETGLFARNTQQLPKVGELQNLTIGSLALSARKPV
                     PDKIFTQADAAFVFAFKESQAADMAQFEKDKGQLMQQALAEARQRILLKFKDELKAKA
                     KIEINSGPLEEI"
     gene            4539..5573
                     /locus_tag="VF089_15450"
     CDS             4539..5573
                     /locus_tag="VF089_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6175402.1"
                     /translation="MFDALFGVFSNDLAIDLGTANTLIYVKNQGIVCNEPSVVAVQQR
                     NERGGKKVLAVGAEAKRMLGRTPGSIVAIRPLKDGVIADFEITEAMLRYFIQKVHNRR
                     TLVRPRIIVGVPFGITEVEKRAVRESAESAGAREVYLIEEPMAAAIGSGLPITEPTGN
                     MIVDIGGGTTEVAVISLSGIVFSRSVRVGGDKMDEAIAQFIKRKYNLLVGERTAELIK
                     ITIGSAYPGDEIQTMEIKGRDLVAGVPKTVVITDEEIRDSLLEPINQIVEAVRLSLER
                     TPPELASDIVDRGIVLAGGGALLRNLDILLREETGLPVMMADDPLTAVVMGAGKALDE
                     ISLLREVAVH"
     gene            5619..6452
                     /gene="mreC"
                     /locus_tag="VF089_15455"
     CDS             5619..6452
                     /gene="mreC"
                     /locus_tag="VF089_15455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6175403.1"
                     /translation="MLAFLRKYQIPLSCCFCIVLSLYIMTAAARGQLKNDPIGPLLLW
                     LIRPLQIAAHSTTSWIKGLQESHLTVAGYKAENERLRKRILELEMERNRFLEAEATNQ
                     HLRHLLDFRSQLPAASITASIIANSASNWFQSCSIDKGTSDGVRKGMSVVTPLGVVGR
                     VVDVAARTAKVLLLTDPNSGIDVIVQRTRARGIVSGSLENGTVLKYIKRTEDVQVGDR
                     LITSGLDGVFLKGLMVGTVTKVRKESLGLFQVIEVMPAVSLARTEIVLVVSAEGRSAA
                     K"
     gene            6473..>6832
                     /gene="mreD"
                     /locus_tag="VF089_15460"
     CDS             6473..>6832
                     /gene="mreD"
                     /locus_tag="VF089_15460"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6175404.1"
                     /translation="MKLSLLLFVTGLLMVLLQTTFLHFFPLVPDLILVLCVYWGLHHP
                     TVGAVLAAFVLGYSVDVVSSPILGINAFAMSLVFLAVYLSSRSIWLHNHLVSAVVVLL
                     ASLVKGVALVLVTAVFLS"
BASE COUNT         1396 a         1766 c         1847 g         1823 t
ORIGIN      
        1 gatcagttgt tcttgccgtg acagtgctgt caggaagtcc aagcgcccaa gatcttggta
       61 aagcaatctt ctcatgaatg gtcttagtct tatccaccac ggccggatct ttcaagttag
      121 tgcggacagg catagtagaa cgtttgacat gaaatgatag tgcccgatag attctgccga
      181 atccataaaa cggattttcg atcatatgcc gcaacctcct ttcaacccag catggtttgt
      241 tcgtaaagat ctcgacggct ttttcggatt gatgatcgat aatctcattc agctaatcct
      301 gattgtcagc ctatgccgcg aactgatcca gcttcccgac gaatacattt tcggcaggat
      361 tctgcccggc gccgccgttt ccatattggt tggcaacctg ttttacgcat ggcaggcgcg
      421 gcggctcgcg cgcgaagtcg gtcgggagga cgtcaccgcg ctgccttacg ggatcaatac
      481 ggtcagcctg ttcgcgttta tcttcttcat catgatgccg atctatcagg agacgcagga
      541 ccccatttgg gcatggaagg ttggtttggt cgcttgcttt cttaacggcg cgattgaaat
      601 tgccggagcc tttgtcgccg accgtgtccg tagcgtaacg ccgcgggctg cgttgctttc
      661 agctttggcc ggcatcgcga tcactttcat ctcgatggat tttaccttcc gcatcttcgc
      721 gcagcctttg gtggcgctga ttccgctggc attgattttt atcgcgtact tttcacagca
      781 aagatttccg ctcggccttc ccggtggaat ggtggctgtc gccgttggaa cgctattggg
      841 atggtggctc ggagctgtaa agggcccggc cagcgtcaat ttcgcgctgg cacggcccga
      901 tttcaccggc ggttcgttac tcgaggcgct gctacatccg gcatttttga attatctgtc
      961 agtgattatt ccgatgggaa tatttaatgt cgtcgggtca ttgcagaata tcgaaagcgc
     1021 tgaagctgcc ggagaccgct acccggcctt tccatcgctc ctggccaatg gcgtggggtc
     1081 cgtggtggcg gctttgctcg gcagcgtttt tccgactacg atctacatcg gccatccagg
     1141 ctggaaacgg ctcggcgcgc gctccgggta ctcggtttta aacggtcttt tcatcgcctc
     1201 gatctgcctt accgggacca ttcaagccgt gcttgggttt attccgctcg agtccggcat
     1261 cggcattctt ctttatatcg gtgttgtcat cgttgcccaa tcttttcagg agacgcccaa
     1321 ggaacatgcg ccggcagtgg ctctgggaat tgtgccggca ttggccgcgt gggggctgct
     1381 aatggtggag actggtttgc gcgtcgccgg caaatcgttg cactcgattg gacttgcgcc
     1441 ttttggccaa aaccccgcga tgcatggcat gatctcgctt cagcaaggat ttattttcac
     1501 ctcgatgatt cttgcagcca tcggtgtggc gttaatcgaa agacaattta aaaaagcggc
     1561 tgcctggtcc ttagttgcag ccgttttctc cgcgattgga cttatccacg cctacgaatt
     1621 gacgcccgca ggcgtagccg cgcggttcgg cttgcttgta gctccagaat ttgctgccag
     1681 ttatttactg ctcgccttgt tattctttgc cttcgcgttc gctccaggta agcggcataa
     1741 aaacgccaaa ccgggttctc cgtaaaaaag gcctcggcta tcaaatcgtc tatggccgcg
     1801 gcaaggtgta gggcttgcta ttcacgtgag tgacttccca gagaccattg cggtgctgag
     1861 tgatccgtgt gactccgcag ggatcgagat ggaatgagcg gtagttattc agatcggttc
     1921 cagttacccg gcaaacgacg ccaaggatag ggaagttatg acttaccacg accaccgtct
     1981 gatctgtttg atggcgctgc gcgatgcgtg acaaagcgcg ccaagccctt tcggccacat
     2041 cggcgagcct ttctccgccc ggtatctgaa gttccgcggg cgcggttctc cagatcgtta
     2101 ggaaatcgga atacttcgcc ttgatttcgt taaaagtcag tccctccaat tgaccatggt
     2161 cgagctcacg tacgtcatcc tcgatcatca ccggaaggtt gtgcggccgg ctcaccagcg
     2221 ccgccgtttg gcgcgctctc tcaagactgc tcgagtaaat ggcatgaata gtttcgttgc
     2281 cgagctgaat ggcgatctgc tcggcctgac gcaggcccac ttcggtaagc cggcgatcac
     2341 tcgaaccttg acagcggcct tgaagattcc aatccgttgc cccatggcga agcaaaacga
     2401 tttgcatcag cacagaggct caatcagatt tcttcaagag gcccggaatt aatttcgatc
     2461 ttggccttgg ctttgagctc gtctttgaac ttcaagagta tccgctgccg ggcctcagct
     2521 agcgcctgtt gcattagttg acccttatcc ttttcaaatt gcgccatgtc agcagcttga
     2581 gactctttga aggcaaaaac aaaggccgcg tcggcttgtg tgaaaatctt atcgggaaca
     2641 ggtttgcgcg cggagagtgc cagacttccg attgtgagat tttgcagctc gcccacttta
     2701 ggcaactgtt gtgtgttgcg ggcgaacaac cccgtttcct ccagcttcag tttcttatca
     2761 cgagcgagtt ttgcaaggtt cggttctttc ttgaattgat cgagcagatc gttggctttc
     2821 tgcacggcta tttcataggc tttagattcc ttcaaacctt tctcgatttt gccgcggact
     2881 gtgtcgagcg gcggaacaaa tgcttcctta cgttctttta ctttgaggat gtagaaggcg
     2941 ttcggtcctt cgatgaccga actgagttcg ttcgctctca gcgatagtgc ggtcttgtag
     3001 aagtcttgcg ttgggccgat ctccggcaac acttctccct cactaaacag tcgagtcgtc
     3061 cttagcgata agccgctctc ttgcgcgagc ttggagagat cgttgccgga agccgctttt
     3121 tcgcggtcgc gatcggcact ctttgctgcc tcatactttc ccttttcaag ttggagctcg
     3181 cgggtgatca ccggtcttgc ctcggccaag ctctgcgttc tcgcttcctt gacatcttcg
     3241 attttaacga tgtggaaacc caacggcgtt tcaatgggct cgctgatttc tcctttagcc
     3301 aacgcgaata tctgttggtc cagagcttcc ggcagttgtc cttgattgag ccagccgatc
     3361 tctccacctt tttctgcgct gggatccccc gactccttcc tggctaattc ggcgaaattt
     3421 tttccaccgc gcgcctccgc cacaattcga ttcgcacgag ctagagcggt ctccttctgt
     3481 ttggcgtctg ctcccggatc aagacgtacc atgatgtaac ggaccttagc ctgtttaggc
     3541 gtggtaaact tggttacgcg gttggcattg taataatcct cgacctcctt atcagtgagc
     3601 tgcaccgggg tggagaactg atcgaaaggg tatgggatgt attctacttg aactttgagc
     3661 ggctccttga gggactcctt attccgctca taaaattttt ggatctcttc atcagtgacc
     3721 tgtacctcgg ataaataatc actagtcgaa aatcggacga actgcagatt gatcttttcc
     3781 tgttcgaagc gatagcgctc gcgcacctcg gcttcagtta catgagcggc gtcggcgaga
     3841 actccgagta agcgctggat cgtgagctgc tttctctggt cttcttcgaa ctgagctgga
     3901 gtcaagcggt tggcgcgcag aagctgtata tagcgctcct tattaaaccg gccattgaca
     3961 tggaactcgg ggatttgcgc gatggaattc atgagctctt cgtccgtggc cgcgagacct
     4021 aagcgccgtg cttcctgaag cactaaacgg gcctcgatca tctcttccag caacgagtgc
     4081 ttgatgttta aatttttcag cagttcgggc gtaagagagc ctttgaacat ttcgcggtag
     4141 cgttccacgg cccgctgata atgcattacg aattctcgct gcgagatgct ttcaccgttg
     4201 acttcagcga cgtcggaggc tacgcccgtt ggctgttggg agccgccgta gaatgcgaca
     4261 aaaacgacaa taatcaatcc caatagaatg gtgatgatcc aggaccgttt cttttttctt
     4321 agaaaatcga gcatgcgcga caagcctttc agcgttaggt cggaagagcg tacttgagag
     4381 aataataatg atagggaaag gaaattggca atgcaaccat cgcataattc accggttcct
     4441 atgctgttga aagttttcat tttcttgctt gggcgtgaca aatctgctat ttttacgctg
     4501 ctttagtctt ctcttctcgc gctaaataag gagttatgat gttcgatgcc ctgttcgggg
     4561 tgttttccaa cgatcttgcg atcgatcttg ggaccgccaa cacactgatc tacgtcaaga
     4621 atcaaggcat cgtttgcaac gaaccatcgg ttgtcgccgt ccagcaacga aatgagcgcg
     4681 gaggcaaaaa ggttttggcg gtaggcgcgg aagcgaagcg catgctcggc aggacgcctg
     4741 gcagcatcgt agcgatccgg ccgctaaaag acggcgtcat cgctgatttc gagattactg
     4801 aggcgatgtt gcgttacttc atccaaaaag ttcacaaccg ccggactctg gtgcgaccgc
     4861 ggatcattgt cggagtccct ttcgggatca ctgaagttga aaagcgcgct gtccgggagt
     4921 cggcggaatc cgcaggggcg cgggaagttt acctgattga agaaccgatg gccgcagcta
     4981 tcggatcggg tctgccgatc actgaaccga cggggaacat gatcgtcgat atcggtggtg
     5041 gtacgactga ggtagcggtc atttcgctct cgggcattgt tttctcgcgc tcggtgcgag
     5101 tcggtggcga caaaatggat gaagccatcg ctcaatttat caaacgaaaa tataatttgc
     5161 tggtcggcga acggaccgcg gaattgatta agatcactat cggctcggcg tatccgggcg
     5221 atgaaattca aactatggag atcaaagggc gggatctggt ggctggggtg ccgaagacag
     5281 tagtcatcac cgatgaggag attagagact cactgctgga gccgatcaat caaattgttg
     5341 aggcagtccg actttcactt gaacgaacgc cacccgaatt ggcttccgac attgtcgatc
     5401 gcggtattgt actggctggc ggtggggcgt tgctcagaaa tctcgacatt ctgttgcgcg
     5461 aagagaccgg attgcctgta atgatggccg acgatccttt aactgcagtg gtgatgggcg
     5521 caggcaaagc cttggatgaa atttcactac ttagggaggt cgccgttcac taggtttggg
     5581 accgcaatgt ctcctgctcc taaacgttcg agccttcaat gctcgctttt cttcgcaagt
     5641 accaaattcc tcttagttgc tgtttctgca tcgtcttatc cctctatata atgaccgcgg
     5701 ccgctcgcgg ccagctcaaa aacgatccga tcggaccgct tctgctatgg ctaatccggc
     5761 cattgcagat tgcggcgcat tcaaccacga gctggataaa gggactgcag gaaagccatc
     5821 tcacggttgc cggctataag gctgagaacg aacggctcag aaaacgcatc ttagagcttg
     5881 agatggagcg aaatcgattc ttggaagccg aagcaactaa tcagcatttg cgacacttat
     5941 tggattttcg ctcgcaatta cctgccgcca gcatcacggc gtcgattatt gccaacagcg
     6001 cgagcaactg gttccagagc tgctccatcg acaaaggcac tagcgacgga gtgcgcaagg
     6061 gaatgtctgt cgttaccccg ttgggagttg ttggacgagt cgtggatgtg gcggcccgaa
     6121 ccgctaaagt tttgttgctc acagatccca acagtggaat cgatgtgata gtgcagcgca
     6181 cccgtgcgcg ggggattgtg tcgggctcgt tggaaaacgg caccgtgcta aaatacatta
     6241 agcgtactga ggatgttcag gttggcgatc ggcttatcac atcgggtctc gatggcgtgt
     6301 ttctcaaagg cttgatggtc ggcacggtca ctaaggtgcg caaagagagt ttgggcttgt
     6361 ttcaagtcat cgaggtgatg ccggcggtca gtcttgctcg cactgaaatc gtgctggtag
     6421 tcagtgcaga agggagatca gccgcgaaat agcggttccg tgagtcggag tcatgaaact
     6481 ctccttgctc ctattcgtca ctggactttt gatggttctc ttacagacga ccttcctcca
     6541 tttctttccc ctggttccgg acctgatact tgttctttgc gtttactggg ggctgcatca
     6601 tccgacggtc ggcgctgttc tggcagcatt cgtactaggc tattcggtcg atgttgtctc
     6661 aagcccaatt ttgggtatca acgcgttcgc gatgtcgttg gtattcctag ccgtctacct
     6721 cagttctcgt tcgatctggc tccacaatca cctggtcagc gccgtcgttg ttctcttggc
     6781 ctcattggtc aagggcgtcg cgttagtgtt ggtcacggcg gtttttctca gc
//
LOCUS       DASUTG010000669         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_399922, whole genome shotgun sequence.
ACCESSION   DASUTG010000669 DASUTG010000000
VERSION     DASUTG010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399922"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VF089_15465"
     CDS             <1..1685
                     /locus_tag="VF089_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX6175405.1"
                     /translation="EQIIISEIPFMVNKVRLIEQIAALVQEKKIEGISDLRDESDREG
                     MRIVIELKRDAVAEIIINQLYKHTSLQESFGINMLAIVDGRPKLLNLRDALRAFLEHR
                     KEVVTRRTAFDLRKAEERLHILEGLRIALDNLDAVITLIRNANDPKAAKEGLVASFGL
                     SEIQAQAILDMRLQRLTGLERDKILQEHKETVELIAKLLAILADEKEIYKIIVAELKD
                     IKEKFGDERRTQIVARSEEISIEDMIVDEEMAVTISHEGYIKRNPVTLYRAQRRGGKG
                     KIGTTTKEEDFVEYLIIASMHSYILFFTTTGKVYWVKVHELPQASRAAKGKPIVNLLN
                     LEPGERVSAFLSVREFVEGRFIVFATKKGAVKKTDLMLYSNPRASGIRAIGLEDGDEV
                     IGVRLTDGQQELILSTLDGQSIRFKEDQVRPTGRGTYGVLGIRLDPGDAVVSMEMLNR
                     GASILTVSEKGYGKRTEMEEYRLQSRGGKGIITMKATEKTGRIVGGQQVTDDDQLMLV
                     TNNGKIIRLRMRDIRVIGRNTQGVRLIDVEDGERVVSLARLAEKEEDEEEAE"
     gene            1768..>2414
                     /locus_tag="VF089_15470"
     CDS             1768..>2414
                     /locus_tag="VF089_15470"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6175406.1"
                     /translation="MAKLISVIGAGGWGTALATVLGQKGLAVTLWCHGAETYRELEET
                     KQNRSYLTGIMIPDSVSVTRSLDAAVTGKEVVCCVVPSHAVRQVMSEASSYVNSNAII
                     LCGTKGLEEQTSKTMGEVLLEIFGARAAQRLAFISGPTFAIEVAQGLPAALTVAAYSE
                     TIAAEIQALLSTASLRAYTSKDVIGVQMGGVIKNVIAIAAGISDGLGLGHNARAA"
BASE COUNT          592 a          600 c          714 g          508 t
ORIGIN      
        1 aagaacaaat tatcatcagt gaaatcccgt tcatggtcaa taaagtgcgg ctcatcgagc
       61 agattgccgc attggtgcag gagaaaaaaa tcgaaggcat ctccgatttg cgcgatgaat
      121 ccgatcgcga ggggatgcgc atcgtcattg agctcaagcg cgacgcggtg gccgagatta
      181 ttatcaatca gctgtataag catacttcgc tgcaggagtc cttcggcatc aacatgctgg
      241 cgatcgtcga cggacggcca aagcttctca accttcgaga tgccctccgg gcgtttttgg
      301 agcaccgcaa agaggtcgtc acgcggcgca ccgctttcga tctgcgtaaa gccgaagagc
      361 gcctgcatat tttagaaggt ctgaggattg cgctggataa tctggatgcg gtgatcacgc
      421 taatccgcaa cgcgaacgat cccaaggcgg ctaaagaggg cctggtggcg agcttcgggt
      481 tgtcggaaat tcaggcgcag gcgattctcg atatgcgcct ccagagattg accggccttg
      541 agcgcgacaa gattctgcaa gagcacaaag aaacggtgga gctgatcgcc aagctgctgg
      601 cgattttggc cgatgaaaaa gagatctata aaatcatcgt tgcggagctc aaagatatca
      661 aggaaaaatt cggcgacgag cgtcgaacgc aaatcgttgc ccgctccgaa gagatatcta
      721 tcgaggatat gatcgtcgat gaagagatgg ccgtgaccat ctcgcacgag ggttacatca
      781 agcgcaaccc ggtgactttg taccgcgcgc agcggcgcgg cggcaaagga aaaatcggca
      841 cgacgactaa agaagaggat ttcgtcgagt acctaatcat tgcctcgatg cactcctata
      901 ttctcttttt taccaccacc ggcaaggttt actgggtgaa ggttcatgaa ctgccacagg
      961 ccagccgggc tgcgaaagga aagcccatcg tcaatttact taatttagaa ccgggtgaaa
     1021 gggtctcagc tttcctctcg gttcgcgagt tcgtcgaggg ccggtttatt gtttttgcaa
     1081 caaagaaggg cgcggttaaa aaaaccgatt tgatgctgta ttccaatcct cgagcgtccg
     1141 gaatacgggc catcggcctc gaagacggag acgaggtaat cggtgtgcgg ctgaccgatg
     1201 ggcagcagga gctgattctc tcgacgttgg atggccagtc catccgcttc aaggaggatc
     1261 aggtccggcc caccgggcgc ggcacttatg gcgtgctcgg aatccgtctt gatccaggcg
     1321 acgccgtggt cagtatggaa atgctcaatc gcggcgccag cattttaact gtctcggaaa
     1381 agggctatgg caagcgcacg gagatggagg agtatcgtct ccagtcgcgt ggcggcaagg
     1441 gcattatcac aatgaaggcc accgaaaaga ccggccgcat agtcggcgga caacaagtca
     1501 ccgatgacga tcagctaatg cttgtgacca ataatggtaa gatcattcgt ctgcgcatga
     1561 gagacatccg ggtgattgga cgcaatacac aaggagtccg gcttatcgat gtcgaggacg
     1621 gcgagcgagt ggtctcgttg gcgcggttgg cggaaaaaga agaagacgag gaagaagcgg
     1681 aatagccttc cccccggagg atctccgatt catctggaat ccgatgaacc ggaagctttc
     1741 taatctcact cgggtcattc acgaattatg gcaaagctga ttagcgttat cggcgctggc
     1801 ggttggggca ccgcgttggc cactgtgttg gggcagaagg ggctcgccgt aaccctttgg
     1861 tgccatggcg ccgagaccta tcgtgaacta gaggagacga aacagaaccg cagttatctc
     1921 accggaatta tgatcccgga tagcgtaagc gttacccgct ctctagatgc ggcagtgacg
     1981 ggtaaggaag tcgtgtgctg cgttgtccca tctcatgcgg tacggcaagt catgagcgag
     2041 gcctcctctt atgtcaactc gaatgcgatt atcctctgcg gcaccaaggg attagaagag
     2101 cagacttcga aaacaatggg cgaggtttta ctcgaaatct tcggcgctcg cgccgcccaa
     2161 agactcgcat tcatctccgg gccaaccttt gcaatcgagg tcgctcaggg attgcctgcg
     2221 gcgctgaccg tagccgcgta ttctgaaacc atcgcggctg aaatccaggc acttctgagt
     2281 accgcaagcc ttagagcgta tacctcgaaa gacgtgatcg gggtgcaaat gggcggcgta
     2341 atcaagaatg ttattgcgat agctgcgggt atcagcgacg gcttggggtt ggggcataat
     2401 gcccgggcgg cgtt
//
LOCUS       DASUTG010000670         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_547956, whole genome shotgun sequence.
ACCESSION   DASUTG010000670 DASUTG010000000
VERSION     DASUTG010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547956"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..822
                     /locus_tag="VF089_15475"
     CDS             352..822
                     /locus_tag="VF089_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175407.1"
                     /translation="MLTTIGTDSVFSQVPFYQGKTITLVIGAGPGGIGDLRARALASV
                     LVKHIPGSLTITFQYMPGAGGRKAANHLYNTARADGLTLFRISSSIIPYAVLGESGVH
                     YDDIDKLVYLGTTEHLLYYLFNTRKQAGLDTLAKLRAASGVRIGSAPLGHTGYI"
     gene            829..1434
                     /locus_tag="VF089_15480"
     CDS             829..1434
                     /locus_tag="VF089_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175408.1"
                     /translation="MTAYLLNMKEPKFIPAYENEDLDVAFARGEVDAQVASTSTVVEH
                     DLVNKKLVDFHATIEVPKGRKDSRFANLNLPDIEEFAKADKEKKLLNMMHGFRSIGTA
                     LLLPPATPKDRVDILKEAVRKTHTDPAFINEYRKLTGGDEPTPLMPDEQAEVVKEIPR
                     DAETIELFKNLPEPDLYRRAKSTGSGVGLAISGTLTWNSNS"
     gene            2208..2843
                     /locus_tag="VF089_15485"
     CDS             2208..2843
                     /locus_tag="VF089_15485"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6175409.1"
                     /translation="MNTYKYRFILLGLMVAALGIVIGILLWIKLAPHARFAASQRSPL
                     EGMSQYGSVPEFSLTERSGKTVGLEQLRGTIWIADFIYTDCQDTCPLQSAEMARLQNE
                     LSAQEVKLLSFSVNPGKDTPSVLSQYADRFKASSDRWLFLTGGQEEIKNLVQNGFRLS
                     VATASADPGVILHSPRFVLIDRQAQIRGYYDSRDNEALQRLRRDVAILVKG"
     gene            2857..>3397
                     /locus_tag="VF089_15490"
     CDS             2857..>3397
                     /locus_tag="VF089_15490"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX6175410.1"
                     /translation="MAKTSKNRPASIHAAAPSTAKQHPNWPILALAIAGMVLSGYLTY
                     SAWQGQSVAGCTIGSACDVVLNSRWSRLLGMPISFWGFLTYALLAAIAWNKTTPSQWK
                     MAWTVTIVGLLYSLYLTGVSLIVLDSACPYCLTSLGLMTAIFAVLLMQRPAKMPGFSW
                     GPWLGKTAGAAVLIIIVLHL"
BASE COUNT          802 a          885 c          884 g          826 t
ORIGIN      
        1 gcgactttgc tagtgttgtc attaatccgc ccctggacaa ttcgcatgat cgcgacaggg
       61 tctccagtct cgtcgagcac aatgactgct tagcctcaag ctagcaattt tctgttggat
      121 ttcggtctcg cgaaggtaaa aatcacacag cgagcaccta gtttgacgat tttaattgct
      181 gacggcaaaa gctgccattt cgcgcttgac ctaacttgtg aatgcgccta tggctgtcaa
      241 atcgcaccat gtaagctttt gcataggaac ttatttaact gaaggagaca tcatgggccg
      301 ctttggactt cactgcgctc acgcgctcgg ttttttttag gggtgttcgt tatgctgacg
      361 acgattggca ccgattcagt tttttcacag gtgccgtttt atcagggaaa aaccataact
      421 ctcgtcatcg gcgccggtcc cggcggaata ggtgacttgc gcgctagagc cctggcgtca
      481 gttcttgtca aacacatccc gggcagtcta acaatcactt ttcaatacat gcctggggca
      541 ggtgggcgaa aggcggccaa tcatctttac aacaccgctc gggccgacgg tctcacgctc
      601 ttccgaatct ccagcagcat cataccttat gcggtcctgg gcgaatctgg tgttcattac
      661 gatgatatag ataagctggt ttacctggga acgacggaac atctccttta ctatctgttc
      721 aatacacgta aacaagcagg gctggatact ctggcgaaac tccgcgcggc ttcgggagtc
      781 cgcattggta gcgctccttt gggtcatact gggtatattt agagccggat gactgcctac
      841 ctgcttaata tgaaagagcc gaaatttatt ccggcatatg aaaacgaaga tctcgacgtt
      901 gctttcgcgc gtggggaggt cgacgcgcag gtcgcttcaa caagtacggt cgttgagcat
      961 gatttggtta acaaaaaatt ggtcgacttt cacgccacca tcgaagtgcc gaaaggacgt
     1021 aaagactctc gttttgcgaa tctgaacttg ccggacatcg aagaattcgc aaaagcagac
     1081 aaagaaaaaa agcttctcaa catgatgcat ggattcagat ctatcggcac ggctctgctc
     1141 ctgccgccgg cgactccgaa agatcgtgtc gacatcctca aagaggccgt gcgaaaaacc
     1201 catacagatc cagcgttcat caacgagtat cggaaactca ccggcggcga tgaaccgact
     1261 ccgctcatgc ctgatgagca agcggaagtt gttaaggaga ttccgcgcga cgctgaaacg
     1321 atagagctat tcaaaaattt gccggaaccg gacctctacc gccgcgctaa gtcaactggc
     1381 tcgggcgtcg ggcttgcgat ctccggaact ctgacatgga actctaacag ctagaggcag
     1441 tcgatgctaa gccgtttttt caatgctcgc ctcagctctc atgaaaatct taatggtaca
     1501 gaagctgaga ttttgaaaga tatcgctcga tctgcgcgct gcgtcagcgc ggtttgaggc
     1561 aaagcgtgac ggtatttccc tggactaagg aagcgaccga gaaagtcgtc caggatggag
     1621 gcaaactgaa gaatggggag tacgagtcgt ggccgcttaa aggtccttcg gaggctcaac
     1681 tttgctcgat tgcgtatcgg cagcaatcgg cttctgcgga gtaggatgct tttggcgagc
     1741 cgcctcctcc tgcgctttga tggcgttgag acgcttctgg aaccaagtcg attgtggcca
     1801 actccagttc tgatctgtgt agctagtagt gggctgtttc attgagttac tcccgtatcg
     1861 ggcctcacag caagcgatat gccagcaacc gacgcgtgat tttcgagcag aatagcgagg
     1921 agaaccacac tgccatgaca ggacttagcc agtctcgctc aaattcgaac agtcgagttt
     1981 gcagcaaggc ggtaggtaga tagcgcctgc gaagcagaga tcctcaatcc tgctccgatc
     2041 actgtcactg atgatccccg caacaagctg cgtccatttc ctagtagcga tacacgacct
     2101 aagaaggcaa gccagctttc ccatgctcac tgtcgtcgtt gccacgtttc ggcctctaca
     2161 caaagggcaa attaatcgat aaacttcttc ccggtaaaga tttgctcatg aatacttaca
     2221 agtaccgatt tatactgttg ggattgatgg ttgccgcact ggggatcgtc ataggcatcc
     2281 tcctctggat caagctggct ccgcatgccc gcttcgccgc tagccagcgg agtcctttgg
     2341 aaggaatgag ccagtatggc tctgtgcccg agttctcatt aacagaaaga agcggcaaga
     2401 ccgttgggct ggaacagctg cgcggcacaa tctggattgc cgatttcatc tataccgact
     2461 gccaggatac ctgcccgctt caaagtgcgg aaatggctag gcttcaaaac gaactgtcgg
     2521 cgcaagaagt gaagctactg tccttttctg tcaatccggg taaagacacg ccttcagttc
     2581 tttctcagta tgcagatcga tttaaagcga gcagtgatcg ttggctgttt ctaaccggtg
     2641 ggcaagaaga gatcaaaaac ctggtgcaga acggcttccg cttgagcgtc gctactgctt
     2701 ctgcagatcc tggagtgatc ctgcatagtc cgagatttgt attgatcgac cggcaagcac
     2761 agattcgcgg ctattacgac agccgcgata acgaggcgct gcagcgcctt cgcagagacg
     2821 tggccatctt agtaaagggt tgaggagttc agataaatgg caaagacaag taagaacagg
     2881 ccagccagca tccacgcagc tgctccgagc acagccaaac agcatccaaa ctggccaatc
     2941 ctcgcgttgg caatcgccgg tatggtctta tccggttatc tgacctattc cgcttggcag
     3001 ggccagtcag tagcgggatg cacaatcggc agtgcctgcg atgtcgtgct caacagccgc
     3061 tggtctagat tattgggcat gccgatttct ttttggggct ttttaaccta cgccctttta
     3121 gccgccatcg cctggaacaa gacgacgccc agtcagtgga agatggcttg gaccgtcacg
     3181 attgtcgggt tgctctacag cctctacctc accggtgttt cactgatcgt tttagactcc
     3241 gcttgcccct attgcttaac ttcactggga ttgatgacgg cgattttcgc cgtgcttctc
     3301 atgcagcggc ctgctaagat gccgggcttt tcctggggac catggctcgg gaaaaccgcg
     3361 ggtgcagcgg ttctaatcat catcgtcctc catcttc
//
LOCUS       DASUTG010000671         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_252666, whole genome shotgun sequence.
ACCESSION   DASUTG010000671 DASUTG010000000
VERSION     DASUTG010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252666"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF089_15495"
     CDS             <1..510
                     /locus_tag="VF089_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6175411.1"
                     /translation="VMERLDRLPLKAGHHGLGNATLTVTQVTSEPAISHTIPDVCRVV
                     LDRRLLPGEDPDAAFREIQEALQDLRGWSIVVTRGTLMYPAEVGRGASIVAAIDSASR
                     ELTKKQSELFYSPAALDAGFLNRNGIETVMFGPGDLRFAHTDQEAVPLQDVRDAAKIY
                     AATAIQLLI"
     gene            826..1374
                     /locus_tag="VF089_15500"
     CDS             826..1374
                     /locus_tag="VF089_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6175412.1"
                     /translation="MRVPEITDREDLPEHQREFYDSIIASRKRIGAPYNFLLHAPDLA
                     ARTAHLIGYALFTTDFPQDIKELAICTVAREMDCAFEWAAHADDALKAGVRQECIKII
                     QDRKAPAGLLEHEAEVVRYVQELLRPPHRITPATFDALKARIGVPRLVELTGIVGAYV
                     GLACSLNAFEVQTPPGRPVLRL"
     gene            1738..3462
                     /locus_tag="VF089_15505"
     CDS             1738..3462
                     /locus_tag="VF089_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6175413.1"
                     /translation="MNAFVQSTGTSRTQGRLLTAVIAAIVLVLLGAPLVILFITSLRP
                     PTALPLDPGVSLDNFGAIFSSPRMFQVLVNTAIYGGGALVLSLSLGATIAFLVERTDI
                     PFRSAIYTAMLVSLAVPTMLKAFGWVLLLSPGLGWINHLLRSGLDLEGPNGPLNIYTM
                     PAMIFVTGIGLSNNAFLMLSPVFRQMNPEFEEAAQTAGSRRLTVLRKITLPLLLPGIL
                     SALIYFGMVLVQVMETALAIGVPGGIRVLSVHIYLLTKAEDGLPMYGLAAAFGVVLLL
                     LALLLMLLYFWLTRQQDRFSVVTGKSFRPRQLKLGLWKYIALGFVMLVIVSYALPIFI
                     LMWASLVKVYQPPSFEALSQISLQSYFRTLTNPQVRQSAVNTLILVVIAPTVSVLIAF
                     CASWFSVKRKMRWVEVFAMTPMGIPNVVLALAVFLTYVMTPIHGSIWVLIMAHIVAHL
                     PFTTRVLSAALLQQHKELEEAARVTGATTVRAVRTVTLPLLFPALLNGWLWAFSATLR
                     DFTFAIFLMTGRNMILPSAMWVLWNVPDISGTAALGTFYMCGFFFVTMAARYFDKRQR
                     KKAGWEDH"
BASE COUNT          746 a          973 c         1030 g          908 t
ORIGIN      
        1 gtgatggaac gtttggatcg actgccgctc aaggctggcc atcacggtct cggcaacgcc
       61 accctgacgg tgacacaagt cacaagcgag ccggccataa gccatacgat cccggatgtt
      121 tgccgcgtgg tgctcgatcg caggctgtta cccggtgagg atcctgatgc ggcgtttcga
      181 gaaattcagg aagcgctcca ggatttgcgc ggctggtcca tcgtggtcac gcgcggcact
      241 ttgatgtatc ccgcggaggt gggcagaggc gcctccatcg tggcggcaat cgatagcgcc
      301 agtcgcgagt tgaccaagaa gcaatccgaa ttgttttatt cgcccgccgc tctcgacgcc
      361 ggatttctta atcgcaacgg catcgagacg gttatgttcg gtcccggtga tctgcgcttt
      421 gcgcacacgg atcaagaagc cgtcccgcta caagacgttc gcgacgcggc gaagatttac
      481 gccgctacgg cgattcagtt gctgatctag ggaatgcggg agtcactccg aagtgtttca
      541 tgagttacac ccgcgcacac agaatcaaaa tagagtacgt ccttcagccc ctaattctct
      601 gacgagtctg tcattcccgc atgcttctag cgggaatcca ggtggaattc ggattggacc
      661 cccgattaaa gcattcgggg gtgacgatct tgggtcgcac cccatcaact tagccgaaat
      721 tttcgaagga ggcgcgaagg acatgcagtg ccgaatgtcg gcgcaaagcg ccaagaaaaa
      781 gaacgccgat gtttatccga cctggcgtgc tcggaggagg caatcgtgag agtacccgag
      841 atcactgacc gagaggacct tccggaacac cagcgagaat tctacgattc gatcatcgcc
      901 agccgaaagc ggatcggcgc tccctataat tttctgcttc atgctcccga tctggcggcg
      961 cggaccgccc acctaatcgg ctatgcgctt ttcaccacgg attttcccca ggatataaag
     1021 gagctggcga tctgtaccgt ggcccgggag atggactgcg cgttcgagtg ggcggcgcat
     1081 gcggatgacg cgctcaaagc cggagttcgg caggaatgca taaagatcat tcaggatcgc
     1141 aaggcgccgg ccggactttt ggaacacgag gcggaggtgg tgcgttatgt ccaggagctt
     1201 ctccggccgc cgcaccggat aacacccgcg acattcgatg cacttaaagc gaggatcggt
     1261 gttcctcgac tggtcgagct gacgggaatc gtcggcgcct acgtcgggct tgcatgctcg
     1321 ctcaacgctt tcgaggtaca aacgccgcct ggccgaccgg ttctgcggct gtaggagatc
     1381 gctgcaaaca acccaaggtt cagcgaaggt caaccatcga gaagccagat aaggcctacc
     1441 ttagagatca gataggtttg cgataacagt cgagtacgtc gtgctgactc tcatttcgac
     1501 atttggaatt ctcaacgtgg aagttgctct gtctcgtcaa cgtggaaact attaaacgtt
     1561 tgaaccgctg cgatcctcaa cggctgacgg agctcatcaa cgtcgaactc tcgactctcg
     1621 gacctccatc gtctctactc cttgatagag acttcagccg attttagtca cgattctccg
     1681 taactatttt caaaagcgat aaagactgat atcgcctgtg gtgattagaa tgactctatg
     1741 aacgcgtttg tgcaaagcac aggaacttca agaacgcagg gccgtctgct cacggcagtg
     1801 atagcggcta tcgtgcttgt gctgcttggt gcgccactcg ttattttatt cattacaagc
     1861 cttcgaccgc caacggcgct ccctctggat cctggcgttt cgctggacaa cttcggggcg
     1921 attttctcaa gtccgcgaat gtttcaggtc ttggtgaata ctgcgatcta tggcggtggc
     1981 gcgcttgtct tatctctgag cttgggtgcg acgattgcgt ttctggtcga gcggaccgac
     2041 ataccgtttc gctcggcaat ctataccgcg atgctggtct cgcttgcggt gcccacgatg
     2101 ttgaaagctt ttggttgggt gctcctgctg tcgcccggtc ttggctggat caatcatctt
     2161 ctgcgcagtg ggctcgatct cgaaggccca aacggtccac tgaatatcta tacgatgccg
     2221 gcgatgattt tcgtcaccgg catcggtctc agcaacaacg cctttttgat gctcagcccg
     2281 gtgttcagac agatgaatcc cgagttcgag gaggcggcgc agaccgccgg cagccggcgc
     2341 cttacggttt tgcgcaagat tactctgccg ctgctgctgc cgggtatttt gtcggcgcta
     2401 atctatttcg gaatggttct ggtacaggtt atggagaccg cgcttgccat cggagtgcct
     2461 ggtggcatac gcgtgctgag cgttcacatc tatctgctca ccaaagcgga agacggttta
     2521 ccgatgtacg gtttggccgc ggccttcggt gtggttttgc tgctgctcgc cctgttgctg
     2581 atgcttcttt atttttggct aacgcgccaa caggacaggt ttagcgtggt tacgggcaag
     2641 tcttttcgcc cgcgccaact caagttgggt ctctggaaat atatcgcctt gggattcgtt
     2701 atgctggtga ttgtttctta tgctttgccg attttcatct tgatgtgggc cagccttgtc
     2761 aaggtttatc agccgccctc tttcgaagcg ctaagccaga tcagcttgca gagctatttt
     2821 cgaacactta cgaatcctca agtgcggcag agcgcggtca atacgctgat ccttgttgtt
     2881 atcgccccaa cagtgtcggt gcttattgct ttttgtgcca gttggttttc ggtgaagcgc
     2941 aaaatgcgct gggtggaagt attcgccatg acacccatgg ggattcccaa cgtagtgctg
     3001 gcgctggcag tattcttgac ttacgtcatg acgccaattc acggcagtat ctgggttttg
     3061 attatggcgc atatcgttgc tcatctgccg tttactaccc gcgttcttag cgccgccctt
     3121 ttgcagcaac acaaggaact cgaggaagcg gcgagagtca ctggcgcgac gaccgtccgc
     3181 gccgtccgga ccgtgacact gccgctgcta ttcccggcgt tgctaaacgg ttggctctgg
     3241 gcgttttccg cgactttgcg cgattttact ttcgcgattt ttctgatgac cgggcgtaac
     3301 atgattctgc cgtcggccat gtgggttttg tggaacgttc ctgatatatc cggtaccgcg
     3361 gcacttggaa cgttttatat gtgcgggttc tttttcgtta ccatggccgc ccgctacttc
     3421 gacaaacgac aaagaaaaaa agccggctgg gaggatcatt gatagatatg gccgctgcgc
     3481 agaaaattcg aaaggagcat gacatgaaca gggtcgatag accaagtaga acgtttgtct
     3541 ggttgttatt tcttgcattg atgggcgcgc tctcgctatc aacgaaggtt gtgtcggctc
     3601 aatcagtgtt cggcaagacc tatgaagagg ttctcgacaa ggccaagcgc gaggccg
//
LOCUS       DASUTG010000672         5355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2030653, whole genome shotgun sequence.
ACCESSION   DASUTG010000672 DASUTG010000000
VERSION     DASUTG010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030653"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1117
                     /locus_tag="VF089_15510"
     CDS             146..1117
                     /locus_tag="VF089_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6175414.1"
                     /translation="MRELNYYEAKFEALDEIMAGDGRVHLINGTFLSLSPHRHVYQNL
                     AKKYRERMISPPISELGFCGLATGAAMAGLRPIVDLSTGSFIYEAWPQVANEAANAYY
                     MSGGQTRVPVVFHIFHGLRGGGAAQHSGSPQAMLWNVPGLEIVLPSTPRDVKGLLKTA
                     VSTDNPTIFVDHVKLLDVRGEVDEDEKPIPFGVADIKRKGSDVTVISTSYTVQRCLKV
                     AEQLAKENIDVEVIDPRTLVPFDLATILSSIEKTHRVVLVDETHQSCGVAAEIAARIA
                     ESGFDKLKTRIRRVSTLDVPVPFSPPLEEYIGPREDRIVAAIRAAVA"
     gene            1497..2489
                     /locus_tag="VF089_15515"
     CDS             1497..2489
                     /locus_tag="VF089_15515"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6175415.1"
                     /translation="MANKRESPEDFIDLLRRMWLIRAFEEKVSALYAERQIVGLLHLG
                     IGQEAVAVGVCGLLRPDDFVFGGHRSHGHALAKGADVNRLMAEIAGRATGYCGGKGGS
                     MHIVALDAGFITATGVVGGTIPLALGAAFAAKENKQGQVSVVFFGDGAGQAGPFHESL
                     NIASLWQLPVIFVCENNGFAEFTPLSAHTKIERLAQHAKTYAIPSSTVDGNDLLAVRL
                     AMSKALEIARTRKGPVFIECLTHRLRGHYEGDPAKYRELSQLSEWKKKDPIARLSRLL
                     KSKKMMTDEGLRAIESEARARVEEAAEFSLSSSWPASEEVATQVMRIDREPFFS"
     gene            2568..3545
                     /locus_tag="VF089_15520"
     CDS             2568..3545
                     /locus_tag="VF089_15520"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6175416.1"
                     /translation="MAEQTFLEAIRAGLHEEMSRDPAVYVFGEDVSLGGPFGVTKGLA
                     EDFGIQRVVNTPISEGTVMGLAIGAATAGLRPLVEIMFIDFITLALDQLVNHAAKLHY
                     MSGGQLAIPLTVRVQCGISGAMGAHHSQSLESWLAHVPGLKVVMPSNPADAKGLLKAA
                     VRDDNPVVFIEHRGLYWGRGNVPDGDHLVPIGKAAVLRHGEQVTIVALANMVGAALAA
                     ADELAANEISAEVIDPRSVSPLDIETIVDSVRKTGRMVVAHEAVEQGGVGAEITAAVQ
                     QHALYYLDAPILRVAAPFAPVPASPALEKEFLPGQKRIVESVKKLIDHP"
     gene            3696..4007
                     /locus_tag="VF089_15525"
     CDS             3696..4007
                     /locus_tag="VF089_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175417.1"
                     /translation="MKQSKTPDDRLRAFKRCIGISIAEEALQLLIKESAMRTVLVSNL
                     VAISIANKKLHAAKCAECGAKMYPSSLLKQHLARHRLNQESLVTELRELQHAIERMRI
                     A"
     gene            complement(4086..4535)
                     /locus_tag="VF089_15530"
     CDS             complement(4086..4535)
                     /locus_tag="VF089_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175418.1"
                     /translation="MENGKQPEAKVVTLRTQLVKEGHTRNLLAETDLMTLRIHCYAPG
                     IGENALHSHTKEDHIFLVLEGTAEFNTGRDGKTVLNASKHQAIVLPAGCYYQFRNSGD
                     TPLVMTRIGAGPDKQDQRLNPDGTPIPGRTHEQGAAKPVLIEGAFFE"
     gene            4651..>5355
                     /locus_tag="VF089_15535"
     CDS             4651..>5355
                     /locus_tag="VF089_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6175419.1"
                     /translation="MQIISTWAYLDFPHPGARMSLLAEITAIFSAMGWASDSVLVRLG
                     LRRSNIFAAMLMSFAVSTACMWTYLIATTSLEFLSSPAMIYFVISGCVQPLLARALFY
                     EGITRIGVARAGPLRGTEPFFATIIGVSFLYEQPGALVYLGTILIMTSLWLISGHQSG
                     AAKWRLLDTAFPISAALISAISQSLRKQGLKILPDPFVAVAIVTTVSLILLLIFVLTT
                     GRAQSLRLDRQGFSFFL"
BASE COUNT         1152 a         1463 c         1526 g         1214 t
ORIGIN      
        1 agagttattg acaggctgtt caaaagatcc cagatgcaag gcgcgcgatg tatcaacgag
       61 cggatgcgta cgcttcgagt acgttggagc gaggcgatca agcgcaacga agcatatggg
      121 tctttttcag cggcctgagg actttatgag agagctaaac tactacgaag ctaagttcga
      181 ggcattggac gaaattatgg ccggcgatgg gcgagtgcac ttgatcaatg gcacgttcct
      241 gagcctgagc ccgcaccgcc acgtctatca gaaccttgcg aagaaatatc gggagcgcat
      301 gatctcgccg cctatttccg agcttggctt ctgcgggctg gccacaggag cggctatggc
      361 cgggctccgt cccatagtcg atctgagcac cggcagcttt atttacgagg catggccgca
      421 agtcgccaat gaagccgcca acgcttatta catgtccggc ggccagaccc gggtaccggt
      481 cgtcttccat atattccacg gactccgcgg cggtggcgcg gcacagcatt ccggcagccc
      541 gcaggcgatg ctgtggaatg tgcccggatt ggagatcgtg ctcccatcta caccgcggga
      601 tgtcaaaggc ctgctcaaga cagccgtttc cactgacaac ccgacgatct ttgtcgatca
      661 tgtcaagctt ctggatgtgc gcggtgaggt tgacgaagac gaaaagccga ttccattcgg
      721 cgtcgcagac atcaagcgta aggggagcga cgtgacggtg atctcaacct catacacggt
      781 ccaacgctgc ctcaaagttg ccgaacaact ggccaaggag aacatcgatg tcgaggtcat
      841 cgacccacgc acgttggtcc cgttcgattt ggcaacgatc ctgtcctcga tcgaaaaaac
      901 tcatcgcgtg gtgctcgtcg acgagaccca tcagagttgc ggagtcgccg cggaaatcgc
      961 cgcgcgcatc gccgagagcg gtttcgataa gttgaagacg cgcattcgcc gcgtctccac
     1021 tctggacgtg ccggtgccct tcagcccgcc gttggaagaa tacatcggtc cgcgagaaga
     1081 tcgcatcgtt gctgcgatcc gcgcagcggt ggcataagct tctacatcta aagaaaactg
     1141 cccagaaaac cgcgcgcaga tagaaacagt caattcacca cgaagatcac gaagagctcg
     1201 aagtaaaacc aatgaatgtc tgaatccttc gtggtacccc ttcgattgcg gttgaggtga
     1261 aagatgcgac ttcgcaaaat cgtcaccccc gaatgtttta gtcgggggtc tagtccgaat
     1321 tccgcctgga ttcccgctag aagcatgcgg gaatgacaga ctcgtcggag aattagggct
     1381 tgagggcgta ctctatttgt ttctgtgtgg gcgagtgtag gctggtgaca cacttggtgt
     1441 gagttagtca tctcctctca accttctctc aagaatcgct cagctcctga tcatccatgg
     1501 caaacaaacg agaaagcccc gaggatttca tcgatctcct gcgccggatg tggttgattc
     1561 gcgccttcga agaaaaagtc tccgcgctat acgccgagag acaaatagtc ggcctgcttc
     1621 acctgggcat cggccaagaa gcggtcgcgg tcggcgtctg cggtctgctg cggccggacg
     1681 atttcgtctt cggcgggcat cgctctcatg gtcatgccct cgccaaagga gcggatgtaa
     1741 atcggcttat ggcggaaatc gccggtcgcg ccaccggcta ctgcggcggt aagggcggct
     1801 ccatgcacat cgtcgcgctg gatgcgggtt ttatcactgc cacgggcgtt gtcggtggaa
     1861 cgataccgtt ggcgctcggc gcggcatttg ccgcgaagga aaataaacag ggccaagtca
     1921 gcgtggtttt tttcggcgat ggcgcgggtc aagcgggtcc gtttcatgag tctttgaaca
     1981 tcgcgagctt gtggcagtta ccggttatct tcgtctgtga gaacaatggc ttcgcggaat
     2041 tcacaccgtt atcggcgcat accaagatcg agcggctggc gcagcatgca aagacttacg
     2101 caattcccag cagcacggtg gacggcaacg atcttctagc tgtgcgtctg gctatgagca
     2161 aagcgctcga gatcgcacgc acacgcaaag gaccggtctt tatcgaatgt ttaacccatc
     2221 gactgcgcgg ccattatgaa ggcgacccgg ccaagtatcg agagttgtcg cagctttccg
     2281 aatggaaaaa gaaagatccg attgccagat tatcacgcct gctcaaaagc aagaagatga
     2341 tgacggacga gggactccga gcgatcgaat cagaagcgcg tgctcgcgtt gaagaagccg
     2401 cggaattctc cctctcatcc tcgtggccag ccagtgagga ggtcgcgact caagttatga
     2461 ggattgaccg cgagcctttt tttagttgag tcccaggtag aacatgtcgc cggcggaaca
     2521 caggtgaaag cagcagctct tcttcaacgt tcgggaacaa atcgaatatg gcagagcaga
     2581 cttttttaga agcaattcgc gctggacttc acgaagagat gagccgggac ccggcggttt
     2641 acgttttcgg tgaagacgtt tcactcgggg gaccgtttgg cgtcaccaaa ggattggcag
     2701 aagacttcgg catccaacgc gtggtcaaca cgccgatcag cgaaggcacg gtcatgggat
     2761 tggccattgg cgccgctacg gcaggattgc gtccgctcgt cgagatcatg ttcatcgact
     2821 tcatcaccct cgcgctcgat caacttgtca atcatgcggc caagctccat tacatgtccg
     2881 gcggccagct ggcgatcccg ttgactgtgc gggtgcaatg cggcatcagc ggcgccatgg
     2941 gcgcgcatca ttcgcagagt ttggagtcgt ggctggcgca cgttccggga ttgaaggttg
     3001 tgatgccgtc aaatcccgcc gacgccaaag gcctgctcaa ggccgccgtt cgagacgaca
     3061 acccggtagt ctttatcgag catcgcggtc tgtactgggg ccgcggtaac gtgccggacg
     3121 gcgatcatct ggtgccgatc ggcaaggcgg cggttctgcg tcacggagaa caagtcacca
     3181 tcgtggcgct ggcgaatatg gtgggagcgg cattggcggc agccgatgaa ttggcggcca
     3241 acgaaatttc cgcggaggtc atcgacccgc gcagcgtttc acctctggat atcgaaacta
     3301 ttgttgactc cgtcagaaaa accgggcgga tggttgtcgc gcacgaagcc gtggaacagg
     3361 gcggggttgg cgcggagatt accgcggcag ttcaacaaca cgcgctttac tatctcgatg
     3421 cgccgattct tcgtgttgcc gcgccgttcg cgccggttcc ggccagcccg gcattggaaa
     3481 aagaatttct cccgggccag aagcggattg tagaatcagt gaagaagtta atcgatcatc
     3541 cctgagcacc gctgcaactc tccgcagccg ttcgtcggtt ccggcaactg catcgccgtt
     3601 caacagttta tagcgccggc atcgaactcg tgacagattg tcgcaacgtc acacgattgc
     3661 tgtcacatcc ggcagtcatt gcaaccggtc taacgttgaa gcaatcaaaa actccggatg
     3721 accgcttgcg cgcgttcaaa agatgtattg gcataagcat tgctgaagaa gcgttgcagc
     3781 ttttaatcaa ggagagtgca atgcgaacag tactggtgag caacctcgtg gccatatcaa
     3841 ttgccaacaa gaaattgcat gcggcaaaat gcgccgagtg cggcgccaag atgtatccca
     3901 gctcacttct gaagcagcat cttgcccggc atcggctcaa tcaggaatcg ttggtaaccg
     3961 aactgagaga gcttcagcat gccatcgagc gcatgagaat agcatgaatg gtgaactgcg
     4021 gcattgcgca atgccgcagt tccggtctag cctcccgcgg gtatttaaag tctaaattcc
     4081 cctactcatt cgaaaaaagc tccttcaatc agcaccggct tggccgcgcc ttgctcgtga
     4141 gttcttccag gaatcggtgt gccgtccggg ttcaaacgct ggtcctgttt gtccggaccg
     4201 gcgccgatgc gagtcatcac cagaggggta tctccggaat tgcgaaactg gtagtagcaa
     4261 cccgctggaa gaacgatcgc ttggtgcttg ctcgcgttga gaacggtttt gccgtcacga
     4321 ccggtgttga attcagcagt tccttcgaga accaggaaga tatgatcctc tttggtgtgg
     4381 gagtgaagcg cgttttcacc gatccccggc gcgtaacagt ggatgcgcaa tgtcatcaaa
     4441 tctgtttccg ccagcaggtt gcgggtgtgc ccctccttca ccaattgagt tcgcagtgtc
     4501 accacctttg cttccggttg tttgccgttt tccataatcc ctcctgttaa ttgttgttgt
     4561 tcctggctcc gaacacgatt ctaggtcagg tcgtcgcgat tgtgcaatac acgtcgattg
     4621 acacccaacg ctccatgacg gattttgcgg ttgcaaataa tcagcacgtg ggcttatctg
     4681 gatttcccac atcctggcgc gcgcatgtct ctacttgctg aaatcacggc tattttctcg
     4741 gccatgggct gggcctccga ttctgttctg gtccggctgg gcttacggcg atccaacatt
     4801 ttcgcagcga tgttgatgag ctttgcggtc tccaccgcct gcatgtggac ctacctgatc
     4861 gccaccacct cgcttgagtt cctgtcctcg ccggcgatga tttattttgt cataagcggt
     4921 tgcgtgcaac cgctgctggc gcgagccctg ttctatgaag gaatcacccg aatcggcgtc
     4981 gctcgcgccg gtccgctgcg cggcactgaa cccttttttg caacgattat tggcgtgagt
     5041 tttttgtacg aacagcccgg tgcgttggtt tatcttggca cgatactgat catgacaagt
     5101 ctctggctga tctccggcca ccagagtggt gcggccaagt ggcgccttct cgataccgct
     5161 ttcccgatca gcgcagcgtt gatttccgcg atctcgcaaa gcttacgcaa gcagggactg
     5221 aaaattctac ccgacccgtt tgtagcggtg gcgatagtga ccacggtttc gttgattctg
     5281 ttattgatct ttgtgttgac caccgggaga gcccagtcgc tgcgcctaga caggcagggt
     5341 ttttcatttt ttctt
//
LOCUS       DASUTG010000673         2936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_400981, whole genome shotgun sequence.
ACCESSION   DASUTG010000673 DASUTG010000000
VERSION     DASUTG010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400981"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VF089_15540"
     CDS             <1..1222
                     /locus_tag="VF089_15540"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HEX6175420.1"
                     /translation="LTFVNNYFRDVNLKTFYRYYDFDNRSKRVLLPEGIIVNDQGTPT
                     GNTYLSFPYSYAKQNLGLDASYNLTRWLTAQVGYVWERMHREIARELLNSNEHTFGPT
                     FVIKPNPSMLFSLSYRRSIRDAHDYDAGRNANVDIGETYEEARALRLEELRKFDQAAR
                     NRDRFTFLTQFSPWETLTLHGGFDFTNDRYPRSDIGITKDIDYAPSLGFVYAPLEWLS
                     IFGDYNWERFDWKMQAIARNTGPGGCPDLELRTSENCPAATWRSRGNDEIHTIKAGSD
                     VQVIKNLLNLRLQYGFSFGSSEVNSSGNSPASGDTGVVPAVDYPTIVNRWHEFLASLE
                     YVLHKNLSLKGGYYFNSYSAKDRGVDIMRVWVGDLDNPSNINTSIGRSFFLGDRGKGS
                     YQAHMGYFGLRVKF"
     gene            1331..2689
                     /locus_tag="VF089_15545"
     CDS             1331..2689
                     /locus_tag="VF089_15545"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX6175421.1"
                     /translation="MMKNNSSWMGPAIVVFAIAAIVGSCAVLGVAPSEISGQRLFSFP
                     GDWEKIPAKTVTLFYPGQTSWQYLTSAAHPGAQAIDAGCGTCHAGQEKALGAKSVQAG
                     PRETDPIDGKPPSLDLTVRAAYDAEFVYFQFRWATKVPHAMHTLWRYDGKQWVAWGGP
                     KPDASKKGIPPSYEDRLAILLDDPNNVTAYDGAGATFSQVGCWMTCHNSMRAMPGDVP
                     RKAIDPHPYWGGKGRRVGDIRKYLLITRTAQDDAGAWDKVKPAAELNKLKTAGKYLDL
                     WQWRAARSNAVGYASDDWVLEYRNGDAGRSPFFNPPKPQFMYDEKLTSFRAIPESELA
                     ARMGKAALIEKKTAVALDPNAKFAVGDLLPQYLQREPEGSAADVQAYGRYVDGHWVVE
                     LRRKLNTGNADDKVLRPGIAYPIGFSIFDDAVSNRRHYVTLPMTLGLGVKGDVTAVKV
                     GP"
BASE COUNT          670 a          802 c          822 g          642 t
ORIGIN      
        1 tctcacattc gtcaataatt acttcagaga cgtcaatctc aagactttct atcggtatta
       61 cgatttcgac aatcgcagca agagagtgct gctgccggaa ggtatcatcg taaacgacca
      121 aggtaccccc accggtaaca cgtacctttc cttcccgtac tcttacgcga agcaaaacct
      181 cgggttggat gcttcgtata atctcacccg ttggctaacc gctcaggtcg ggtacgtctg
      241 ggaaagaatg catcgcgaaa tcgcgcgcga gctgttgaac tccaatgagc atactttcgg
      301 ccctacgttc gtcatcaagc caaacccatc gatgctgttt agcttaagct acagacgctc
      361 tattcgcgat gctcacgatt atgatgccgg gcggaatgct aatgtcgata tcggcgagac
      421 ttacgaagag gcgcgcgcgc tgcgcctcga ggagttgcga aaattcgatc aggctgcgag
      481 aaatcgcgac cggttcactt tcttgacgca gttctctccg tgggaaacct taacgctgca
      541 cggaggattc gacttcacca acgatcgtta tccgcgcagt gacatcggca tcaccaaaga
      601 catcgattat gctccgtcct tagggtttgt ctatgcgccg ctggaatggc tgtcgatctt
      661 cggcgattac aattgggagc gctttgattg gaaaatgcaa gcgattgcac gcaacacagg
      721 ccccggtgga tgtccggatc tggaacttcg aacctcggaa aattgtccag cggcgacttg
      781 gagaagccga ggcaacgacg agatccatac gatcaaggca gggagcgatg tccaagtcat
      841 caaaaacctc ctgaacctgc ggctgcagta cggcttttca tttggcagca gtgaggtcaa
      901 ttccagcggc aacagccccg catccgggga caccggtgtt gtgcctgcgg ttgactaccc
      961 gacgatcgtt aaccgttggc atgagtttct agcctcgctg gaatatgtgc tgcacaagaa
     1021 cctttcgctg aaaggcggat actatttcaa cagctacagc gccaaggatc ggggtgtgga
     1081 tatcatgcgg gtctgggtcg gggacctcga taatccctct aacataaaca cctcaatcgg
     1141 ccgttcgttt tttctcggcg accgtggcaa aggctcgtat caggcccaca tgggttactt
     1201 cggcttgagg gtcaagttct agaaatctcc ttgcttctca gccaatgaaa atactatcga
     1261 cggccgcgaa caaaagaatt cgcggctcgt acattttcga ccgcttcagc aaccaagcgt
     1321 cggaggcgaa atgatgaaga ataactccag ttggatgggt cctgcgatcg tcgtttttgc
     1381 catcgccgca attgttggga gctgtgccgt gcttggggtt gcgccttccg agatatcagg
     1441 tcaacggctt tttagttttc ccggcgactg ggaaaagatc ccggcaaaga cagtcacgtt
     1501 gttctacccc ggtcaaacct cttggcagta cttgaccagc gcggctcacc ccggcgcaca
     1561 ggctatcgat gcgggctgtg gcacctgtca cgcgggtcag gagaaagcgc tcggcgccaa
     1621 atcggttcag gctgggcctc gcgagacgga tcccattgac ggcaagccgc catcccttga
     1681 cctcaccgtt cgcgctgcct acgacgcaga gttcgtctac ttccagttcc gatgggcgac
     1741 taaagtgccg cacgcgatgc acacgctttg gcgttatgac gggaagcagt gggtggcctg
     1801 gggcggccct aaacccgacg cgagcaaaaa aggcataccg cctagctatg aggacaggct
     1861 agcgattctg ttggatgatc cgaacaatgt aaccgcctac gacggtgcag gtgcgacctt
     1921 ttcgcaggtc ggatgttgga tgacttgcca caacagcatg cgcgccatgc ccggagacgt
     1981 cccccgtaag gcgattgatc cacatccata ctggggcgga aagggccgac gggtcgggga
     2041 catccgaaaa tatttgttga ttacgcggac ggcacaagat gacgccggcg catgggataa
     2101 agtgaagccg gcggcggaac taaacaaact caaaacggcc gggaagtatc tcgacctgtg
     2161 gcagtggcgc gcggctcgtt ctaacgctgt cgggtacgcg agcgatgatt gggtgctaga
     2221 gtaccgcaac ggcgatgcag gccgctcgcc attttttaac ccgcctaaac ctcagttcat
     2281 gtacgacgaa aaactcacca gctttcgagc tatccccgaa tcggaactcg ctgcacggat
     2341 gggaaaggcg gcgctgatcg agaagaagac cgcggtggcg ctcgacccga atgcaaagtt
     2401 cgccgtgggt gatctgctgc cgcagtatct ccagcgcgag cccgagggaa gtgccgccga
     2461 cgtccaagct tacgggcggt atgtcgatgg acactgggtg gtcgaactgc ggcgcaaact
     2521 aaataccggc aatgcagacg acaaggttct gcgaccggga atcgcttacc cgatcggatt
     2581 tagtatcttc gatgacgcag tgtccaaccg gcggcattat gtgaccttgc ctatgacgtt
     2641 aggtctcggc gtcaaggggg atgtgacggc ggtaaaagtc gggccgtaaa agcctgcaca
     2701 gtttctccag cgactttttc agaatggttg ggactcgcga tagggtattc ctttccttgc
     2761 ttaaatcgga gccgagcgta aaaccgaccc gtgcgatgtt tctggctttt gtcgtcgccg
     2821 taattggttt cgttacggtg actgtttcca atgcccagcc ggccgatgat aactgcgcca
     2881 cctgccattt agccctgggc attgcaactc tcaccaagcc cgccgaagat tacaaa
//
LOCUS       DASUTG010000674         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_401142, whole genome shotgun sequence.
ACCESSION   DASUTG010000674 DASUTG010000000
VERSION     DASUTG010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401142"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF089_15550"
     CDS             complement(<1..437)
                     /locus_tag="VF089_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX6175422.1"
                     /translation="MLSRRFKQVDVFTEKPFFGNPVAVVIDAAGLDGAEMQHIAAWTN
                     LSETTFVLPAASGDADYQLRIFSPKQELPFAGHPTLGSAHAVIESGFATTHDGRLRQE
                     CLAGLIDLTIEETASGKRIFLEAPDAKISVVDDNNARSMQTGLG"
     gene            complement(464..1069)
                     /locus_tag="VF089_15555"
     CDS             complement(464..1069)
                     /locus_tag="VF089_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175423.1"
                     /translation="MMKKIWSSNLWGVVLLSLSASSSFAVALSQEDGNRLQAKIDAIV
                     KNAAADPPKPRKFAIAEQEANSYLVFNLKEKIPKGLAEPEITMVGDGALAARVLVDMD
                     EVKRRRQSRSVIDPLNYLSGQVPLNARGLLRTREGRGQLYLRSADIAGVPLPKPVLQE
                     LIGFFSRTAQNPKGFDIDAPFDLPSKIREISVRAGESVVIQ"
     gene            1227..2900
                     /gene="ilvD"
                     /locus_tag="VF089_15560"
     CDS             1227..2900
                     /gene="ilvD"
                     /locus_tag="VF089_15560"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX6175424.1"
                     /translation="MADNLKPRSHVITDGPDRAPTRAMLKAVGFTDEDLSRPIVGVAN
                     TWIEIGPCNFHLRRLAAKVKEGIRAAGATPMEFNTVSISDGISMGAEGMKCSLISREV
                     IADSIELVARGNHFDALVCLSGCDKTNPGVVMALARLNIPSLALYGGSIAPGHLGDRD
                     LTIQDVFEAVGAFSSGRIEEEQFKSIENTACPGAGACGGQFTANTMSTVMEFLGISPM
                     GSNGIPATAAEKDEAAFDAGKLVVELLRRDMRPSQILTRKSIENAIASVAATGGSTNA
                     VLHLLAIAREAGIELNIDDFDRISAGTPLIADLKPGGRFVANDLYKAGGIKLVAKRLI
                     EAGLVHGDTLTVSGKTLKEAAQGAEEATGQEVVRLMVNPLKPTGGLVILKGNLAPEGC
                     VVKVAGHERLIHKGPARVFDREEDAFAAVQKGQIKADDVVVIRYEGPKGGPGMREMLG
                     VTAALVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAAQGGPIAALRDGDTIIFDIA
                     ARRLDVQISEEEMNTRLRRWQPPAPRYTSGVMAKYAKLVSSASEGAVTL"
     gene            3140..3439
                     /locus_tag="VF089_15565"
     CDS             3140..3439
                     /locus_tag="VF089_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175425.1"
                     /translation="MAEWTLWATLFTVGTALALLALYLICRERLETISSRIAALEKEI
                     EVARLREEANEKNLSKILDAVMELRGSTESHLENLRHGLDQLRAENYSQQSVDAA"
     gene            complement(3563..>3885)
                     /locus_tag="VF089_15570"
     CDS             complement(3563..>3885)
                     /locus_tag="VF089_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175426.1"
                     /translation="VKVSPEDAYGLVNPKAFQEVPKEQIPANGMKIGATLAAKGPHGQ
                     EIPVRIHEIKEKTVVLDLNHPMAGKTLLFDIKILDIQPSVTSSSPQPAKPAPAKPAQT
                     PQPK"
BASE COUNT          848 a         1022 c         1152 g          863 t
ORIGIN      
        1 cccaacccag tttgcatcga gcgtgcattg ttgtcatcga cgacgctgat ttttgcgtcg
       61 ggagcctcaa ggaaaatgcg cttaccactg gctgtctcct caatcgttaa gtcaatcaga
      121 cctgcaagac attcctggcg caacctgcca tcatgggtcg ttgcgaaacc actttcgata
      181 accgcgtgag cgctgcctag ggtcgggtgg ccggcaaacg gcagttcctg cttgggtgaa
      241 aatattcgta gctggtagtc ggcgtctccg gatgccgccg gcaagacaaa cgtcgtctcg
      301 gaaagattcg tccaagcggc aatgtgctgc atctctgcgc catctaagcc ggccgcatcg
      361 atcacgaccg caacaggatt accgaagaaa ggcttttctg taaaaacatc cacctgtttg
      421 aaacgacgag atagcatttc tctaccaaca ggtttggcga gcgctactga attaccacgc
      481 tctcaccggc ccggaccgaa atctcacgga tttttgacgg aagatcgaac ggcgcgtcga
      541 tgtcgaagcc cttggggttt tgcgctgtgc gcgagaagaa gccgataagc tcctgcaaca
      601 ccggcttagg caacggcact ccagcgatat cggcggagcg caaatacaat tggcccctgc
      661 cttcgcgggt ccgcaagagg ccccgcgcat ttaacgggac ttgacctgat aagtagttga
      721 gcggatcgat cacgctgcgc gattggcggc gtcgcttaac ttcatccatg tcgaccagca
      781 cccgagcagc aagcgcgccg tcgccaacca ttgtgatctc cggctcggct agacccttgg
      841 ggattttttc cttcagattg aaaaccaggt acgagttagc ctcctgttca gcgatcgcaa
      901 attttctagg cttcggcgga tcggcggcgg cgtttttcac aatcgcatcg attttggcct
      961 gcaggcggtt gccgtcctct tgcgacagcg caacggcaaa gctgctcgag gcggacaagc
     1021 tcaagagaac gacaccccat aaattgctgc tccagatttt tttcatcatc aacatcgtac
     1081 caggttacga tttgatcgga agctctacaa gcgagctaat tggagtggga tgccgaacct
     1141 ctccagatga tggacggtta aggcgagcct tgcaaattct cctggcccat agttaatttg
     1201 aagactcatc agagtaggag aaatcgatgg cagacaattt aaagcctcgc agtcatgtta
     1261 tcaccgacgg accggaccgc gcgccgacgc gtgcgatgct caaggccgtc ggctttacgg
     1321 acgaggacct ttctcgccca attgtcggcg tagctaacac ctggattgag atcgggccgt
     1381 gcaattttca cttgcgtcgg ctggcggcta aagtgaagga agggattcgc gccgccggcg
     1441 ctaccccgat ggagttcaac accgtctcca tatccgatgg cattagcatg ggtgcggaag
     1501 ggatgaaatg ctcgttgatc agccgtgaag tgattgctga ttcgatagag ctggtcgcca
     1561 gggggaacca tttcgatgca ttggtatgtt tatcgggttg cgacaagacc aatccgggcg
     1621 tggtcatggc gctcgcacgg ctcaatatcc ccagcctggc tctttacggc ggctcgattg
     1681 cgccgggcca tctcggtgac cgtgatttga cgatccaaga tgtcttcgag gcagtgggcg
     1741 ctttcagcag cggccgtatc gaagaagagc aattcaaatc gatcgagaat accgcctgtc
     1801 ccggggccgg agcgtgtggc ggccagttta ccgccaatac tatgtcgact gtaatggagt
     1861 ttctcggaat ttcgccgatg ggcagcaacg gcattccagc taccgccgcg gagaaggatg
     1921 aagcggcatt cgacgccggt aagctggtcg tcgaactttt gcggcgtgac atgcggccgt
     1981 cgcagattct cacccgcaaa tcgattgaga atgcgatcgc ctcggttgcc gccaccggcg
     2041 gctcgaccaa cgcggtcttg caccttctcg cgattgcacg cgaagccggt atcgaattga
     2101 acatcgatga tttcgaccgt attagcgcag gtacgccgct gattgccgat ctgaaaccgg
     2161 gtggccgctt cgtcgccaac gatctctaca aggcaggtgg catcaaactg gtggcgaaac
     2221 ggttgattga agccgggctg gtccatgggg acacactcac ggtcagcggc aaaaccctca
     2281 aagaggctgc gcaaggggcg gaagaagcaa ccggacaaga agtcgtgcga ctgatggtga
     2341 atccgctcaa gcctaccggc ggattggtta ttttaaaggg taatctagcg cccgaaggtt
     2401 gtgtggtgaa agttgccggt cacgagagat tgattcacaa aggcccggct cgcgtgttcg
     2461 atcgcgagga ggacgccttt gcggcagtgc aaaagggaca gatcaaagcg gatgacgtgg
     2521 ttgtcattcg ctacgagggt cccaagggcg ggcctgggat gcgcgagatg ctcggcgtca
     2581 cggcagcgct ggtgggggcc gggttaggtg attcggtagc cctgctcacg gatggtcgtt
     2641 tttccggagc tactcatggg ctgatggccg ggcacgttgc tcccgaagcc gcgcagggcg
     2701 gtccgatcgc cgcgctgcgc gacggtgata cgattatttt cgatattgcc gctcggcgtc
     2761 tcgatgtgca gatttcagag gaagaaatga acacgcgttt acggcgatgg cagcccccgg
     2821 cgccacgata cacaagcggc gtgatggcaa aatatgccaa gctcgtttct tccgcatctg
     2881 aaggagctgt gacgctctag cagtctagat aaaacgcgtt agttaatttc gatgcggctg
     2941 aagcgaccgg cacgtgaaag cgagagtttg ctgaaaaact ctgcttgcag gctgctcaaa
     3001 aagagttaga gccgaggcgc gcgagagaat cggcgagcgg aggcgtacta gcgagtacgt
     3061 tgtagcgaga cgattgagcg caacgacgga tatgactctt ttcagcagcc ctgctacagg
     3121 cgccgggaag agggttaaca tggcagaatg gactttatgg gcgacacttt tcactgtagg
     3181 cacggccctg gcactgctgg cgttgtacct gatctgccgt gagcgtttag aaacgatcag
     3241 cagtcggatt gccgcgctgg agaaggagat tgaggtggcc cggttgcgcg aggaagcaaa
     3301 cgagaagaat ctgagcaaaa tcctcgacgc tgtgatggaa ttgcgcggat ccaccgagag
     3361 tcatttggag aatctgcgcc atggtttgga tcaactacgg gcggaaaact attcgcagca
     3421 atctgtcgat gccgcgtaat tcaacaacaa gatgtttcgt gttcgtgccg cgcgatcgtc
     3481 tatgagttgg tgatcttaac cgacccgatc acgggttcct gctgcgagtt gtcttcagct
     3541 gcgatctccc tcgaccgact gattatttcg gctgcggtgt ttgcgccggt ttcgctggcg
     3601 ccggtttggc cggctgcggc gagctggacg tcaccgatgg ttgaatatcg agaatcttga
     3661 tatcgaagag aagcgtcttg ccggccatcg gatggttcag atccagtaca accgtctttt
     3721 ctttaatctc gtgaatacgt accggaattt cctgcccgtg cggtcctttg gctgccaggg
     3781 tggcgcctat tttcatgcca ttagcgggaa tctgctcctt gggcacttct tgaaaagctt
     3841 tcggattgac caagccatag gcgtcttcag ggctaacttt gactc
//
LOCUS       DASUTG010000675         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1586009, whole genome shotgun sequence.
ACCESSION   DASUTG010000675 DASUTG010000000
VERSION     DASUTG010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586009"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VF089_15575"
     CDS             complement(<1..459)
                     /locus_tag="VF089_15575"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6175427.1"
                     /translation="MSNLEGQQAPAFTLEGSDGKRHSLSDYRGKNVILYFYPKDDTPG
                     CTKEACGFRDSIDSVTNSNAVVLGVSKDGVESHNQFIAKYNLPFTLLSDPETKVMQAY
                     GAWGERKNMEGKTVEGAIRSTVVIGPDGKVLRQWAPVTNAEEHPREVLQFL"
     gene            complement(596..1549)
                     /locus_tag="VF089_15580"
     CDS             complement(596..1549)
                     /locus_tag="VF089_15580"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX6175428.1"
                     /translation="MVKLKGDLDLHKLEVFFWVAELKSFSEAAQRLSLKQPTVSAHIQ
                     ELEKMLGGKLFYRIPGKVTLTALGILLLEQAKDLLAFKRETVAKIERFHGSLSGELWI
                     GGSNIPGEYLLPQRLAAFIQKHPRVKPILRIGDSAGIVDEVLDGKVEVGFVGFKNAEN
                     RLSFEKIWQDEMVLTVPSGHPWARRKSVELSDLRSEQFISRERGSGTLDSFRHLVSKG
                     RRSSDELLSVAMELGSTAAVKEALISGFGISILSRVSVERELAEGLLAEIPIRHFKMI
                     RDFYEVHYKGRPLSPVAQAFREFVKADSSLKHHGNERRKIM"
     gene            1639..2748
                     /locus_tag="VF089_15585"
     CDS             1639..2748
                     /locus_tag="VF089_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6175429.1"
                     /translation="MSKQSRLVSVHHANGAILTEYDGWSLPRHFGNPHEEYLSVRRAV
                     GLLDFCNRAIFEFTGADRLSYLQGLISNDLRLPSSGEGIYAAFLSQQGKVLGDCRVLT
                     TDDRFIVDIWEPLKPKILDHLHRYLVADEVEIADLSDRCAILSLQGPKSETLLEGFLP
                     RDQQPQTNLTHSIAEIGGVEMRICRSSHTGEDGFDLMIPLAGIESMARQLTEVASTYS
                     ARWVGTEAYEILRIEAGIPRYGVDITDENLILETGLTHAVSFNKGCYLGQEVVERIRS
                     RGHVNKNLTGLIIHDKKPPPAGSKILSAEKEIGTITSSVYSPTLESPIALGYVHRDHC
                     SRGTQLLIRYDAELLVNATVTELPFVGTLQNGKRS"
     gene            2870..3175
                     /locus_tag="VF089_15590"
     CDS             2870..3175
                     /locus_tag="VF089_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6175430.1"
                     /translation="MANFVKVCKAEDIKGGCAKSLEINGKSIGVYNVDGKFYAINDIC
                     GHRGGPLAEGELDGTTVICPWHGWRYNVTTGENELLPALPIEKYEVKVEGDDVLVDV"
     gene            complement(3244..3711)
                     /locus_tag="VF089_15595"
     CDS             complement(3244..3711)
                     /locus_tag="VF089_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3571..3573),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6175431.1"
                     /translation="MAMPGIGQKAPEFTLPISREHNVSLKEYHGSKNVVLAFYPLDFT
                     GGUERELSSFEKDRKEFEVRDAQVLSVSVDSVFSHKVFAERMGGISFPMLSDFWPHGA
                     VCAQYDCLRPQGMPKRAVFIIDKEGTIRFAREYDKGIPENSDLFNELDKINQS"
BASE COUNT          918 a         1005 c          974 g          958 t
ORIGIN      
        1 aagaaactgc agcacttcac gcggatgctc ctcggcatta gtcaccggcg cccattggcg
       61 taacactttg ccgtcaggac cgatcaccac cgtcgagcga atagcgcctt cgacggtttt
      121 accctccata ttcttcctct caccccaagc accgtaagcc tgcatgactt tggtttcggg
      181 atctgaaaga agagtaaacg gcaagttata tttagcgata aattggttgt gcgattcgac
      241 tccgtctttg ctcacgccca aaacgacggc gttggaattc gtgaccgagt cgatggaatc
      301 acgaaaaccg caagcttcct tggtgcagcc gggagtgtca tctttcggat aaaaataaag
      361 gatgacgttt ttacctcggt aatcggacag agaatgtcgt tttccgtcgc taccttccaa
      421 cgtaaatgcc ggcgcctgct gcccttctaa attactcatg ggaacctcct attcgagatt
      481 tggctatttc ttttttctat catctataga gacttggtca ataccagccc gcgataagtc
      541 ttgtgtgtcg catggtcgcg ggaatagccg agctggcgaa gcgcactgga gatatttaca
      601 taatcttacg gcgctcattg ccgtggtgct tcaacgagct atctgctttt acaaactcgc
      661 ggaatgcttg agcaaccggt gataacggcc ggcctttgta gtgaacttcg tagaagtcgc
      721 ggatcatctt gaaatggcgg attgggattt ccgctagcag gccttcggcc aattcacgct
      781 ccactgaaac gcgggacaaa atcgaaatac cgaaacccga gatcaaggcc tcttttaccg
      841 ctgccgtgct tccaagctcc atggctacac tgagcagttc gtcggatgaa cgtctgccct
      901 tgctaacaag gtgacgaaaa gaatccaagg ttcccgagcc gcgttcacgc gagatgaact
      961 gttccgagcg caaatctgac agctcaacgg attttcggcg ggcccatgga tgtccactcg
     1021 ggaccgtgag taccatttcg tcctgccaga tcttctcaaa ggaaagtctg ttctccgcgt
     1081 tcttaaagcc gacgaatccg acttcgactt tgccgtcgag gacctcgtca acgatgcctg
     1141 ccgagtcgcc gattcgcagg atgggcttta ctcgaggatg cttttgtatg aacgccgcaa
     1201 gcctttgcgg cagtagatat tcaccgggaa tgttgctgcc gccgatccaa agctcgccgc
     1261 ttaggctacc gtggaagcgc tcgatcttag ccaccgtttc tcttttgaaa gccagcagat
     1321 ccttagcctg ctccagcagc agtattccta atgcggtcaa agtaactttc ccgggaatcc
     1381 gatagaacaa cttgccgcca agcatttttt ccagttcctg gatatgcgcg ctgactgtgg
     1441 gttgcttcaa cgatagacgt tgtgccgctt ctgaaaagct tttaagctct gctacccaga
     1501 agaagacttc cagcttgtgt agatccaaat cgccttttag ttttaccatg gtaaagttga
     1561 tcggctgtga tttttgctat taaatgcgcc gggatcgaac gttgtcaact gcgcaacttg
     1621 gcggtatatt cttgatcaat gtcaaagcaa tcgcggttag tcagcgttca tcacgccaat
     1681 ggagcgatac taacggagta tgatggctgg tcgctgcccc gacatttcgg caacccgcac
     1741 gaggagtact tatcggttcg ccgcgcggtc ggtcttttgg acttctgcaa tcgtgccatc
     1801 tttgaattca ccggcgccga tcgtctatct tatctgcagg gcctgatctc aaatgatttg
     1861 cggctgccat cctcgggaga aggaatttat gctgcgttct tgagccagca gggaaaagtt
     1921 ctcggcgatt gccgcgtctt gactacagac gatcgcttca tcgtcgatat ttgggaaccg
     1981 cttaagccga aaattctcga tcatctacac cgttatttag ttgccgacga ggtggagatc
     2041 gccgatttaa gcgaccgctg cgccatctta tcccttcagg gacccaagtc ggaaacactg
     2101 ctagaaggat ttctcccccg ggaccaacag ccgcagacaa atctaaccca ctcgattgct
     2161 gaaattggcg gagtggaaat gcgcatctgc cgctcaagcc ataccggtga agacggtttc
     2221 gatctgatga ttcctctcgc cggcattgaa agcatggccc ggcagttaac cgaagtcgcc
     2281 tcaacgtact ctgcccgatg ggttggcact gaggcttacg agatcctgcg aatcgaagca
     2341 ggtattccgc gttatggcgt cgacattacc gatgagaatc tcatactgga aaccggtttg
     2401 actcacgctg tgagcttcaa taagggctgc tatctgggtc aggaagtcgt cgaaaggatt
     2461 cgttcccgcg gccacgtcaa caaaaatctt accgggctaa taattcacga caaaaagccc
     2521 ccgccggcag gcagcaagat actgtcggcc gaaaaggaaa tcggcacgat tacaagttcc
     2581 gtctactccc cgacgttaga gtccccgatc gcgctgggtt atgttcatcg cgaccattgt
     2641 tctcgaggta cacagctttt gatacgctac gatgcggaac ttcttgtaaa cgccacggtt
     2701 acggagttgc cgtttgtggg taccctgcaa aacgggaaac gatcgtgatc gtgtcaaggg
     2761 gatacagagc gtttcgaacc agcctgtcca gaatactcgg tacttgaatc ccgccgtcga
     2821 ctccgtatat tgaacgaaaa atttgcgcca cgagaaaggg caaagagaaa tggctaattt
     2881 tgtcaaagtc tgcaaggccg aggatattaa aggcggttgt gccaagagcc ttgaaattaa
     2941 tggtaagtcg attggcgtct acaatgtgga cggcaagttt tacgcgatca atgacatttg
     3001 cggtcatcgc ggcggaccgt tagccgaagg tgaactggac ggcaccacgg tgatctgtcc
     3061 ttggcatggc tggcgctaca atgtcaccac cggtgaaaac gaattgctac cggcgctgcc
     3121 gattgaaaaa tatgaagtaa aagttgaagg cgacgacgtg ctggtggacg tttaagaatc
     3181 attccgaaat atacaaggcc cgctgccatc attgatggag cgggcctttg ctaatgacct
     3241 tatctagctt tggttgattt tgtccagctc attaaataga tcagaatttt ctggaattcc
     3301 cttgtcgtac tctctagcga accgaatcgt cccctctttg tcgataatga agaccgcgcg
     3361 ctttggcatt ccctgcggcc gtagacaatc atattgagcg cagactgcgc catgaggcca
     3421 gaaatccgac agcatcggga aactgatgcc acccattctt tcggcaaata ctttgtgaga
     3481 aaaaaccgaa tcgacactta cgctcaagac ctgagcgtcg cgaacttcga attccttgcg
     3541 gtctttttcg aagctgctta gctcacgctc tcacccgccg gtgaaatcca gcggataaaa
     3601 cgccaacact acgttcttgc tgccgtggta ctccttaagt gaaacattgt gttcgcgtga
     3661 aatgggcaag gtgaattcgg gcgccttttg cccaatgcct ggcatcgcca tatcgacctc
     3721 cttcgctata aagtgaggct cgggtttagc agctctaggc tttcaagtaa agcttgatgt
     3781 agtccatgag ggcatcccgc gtgctgtcga aggcgagatc cgcccaagga atctggctgg
     3841 cgacgaatgt agctg
//
LOCUS       DASUTG010000676         6815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2181477, whole genome shotgun sequence.
ACCESSION   DASUTG010000676 DASUTG010000000
VERSION     DASUTG010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181477"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VF089_15600"
     CDS             <1..525
                     /locus_tag="VF089_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6175432.1"
                     /translation="KKKTVGNGMSKETRMGPIHLESQRKEVEEQVEDAKSRGAKTLFG
                     GERPKGGDLDKGFYYTPTLMSNVPEDSRMTTEEVFGPALPVYTFKTLDEALEKANSSE
                     FGLGSSIWTRNLLSANKAIDKLQAGNVWVNSLHYGYDELPFGGVKSSGLGREHGPEAL
                     DYYFEPKGVVIVNV"
     gene            699..1379
                     /locus_tag="VF089_15605"
     CDS             699..1379
                     /locus_tag="VF089_15605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6175433.1"
                     /translation="MTSENDEEYFACLKVSPTQMDFLWAEGWRHFGIYFFRYQTSFHG
                     DKKFSVMPLRIDLGRFSLTRSQKRVLAKNRDARIIIRPATVNSGKHDLFNKHKVRFKD
                     NIPTSLEDIVSPMPASVPCPSRELCVYLGEELVGVTYLDLGQDATSAVYAIFDPAEAK
                     RSLGILMILHSIQFSRERRCRYYYPGYAYQEPFAYDYKKRFTGLQYLDWDSGWRDYAD
                     HGNSEEAL"
     gene            1526..2329
                     /locus_tag="VF089_15610"
     CDS             1526..2329
                     /locus_tag="VF089_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175434.1"
                     /translation="MFAQMKHMAIVSSNCNQEGDFYRDVFGLKRSGLARAGGAVVVRD
                     GYVGLNLNPRAPGRQAGFDHFGFEVENVETVFARVNEKYPKIKILRRPGNRPFAGISL
                     HDPAGNVFDLSQKGMENRRDLYVDDDETMPRRVSHIALRVLEPDLIAEFYRTVFELME
                     LEKPAGDPNHYLSDGRVTLAIFPWQITHYEGSGIERPALDHIGFKVESMQAFKADLDR
                     YMAANAPTVADRPEKNPEREVRRNLLAKCCFGQFEMSDPDGVLIDVSEA"
     gene            complement(2352..3350)
                     /locus_tag="VF089_15615"
     CDS             complement(2352..3350)
                     /locus_tag="VF089_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175435.1"
                     /translation="MSLTYFLAPALFFFCILWGAGVSAQNFKIYLKTSPVPERLRPFS
                     EPATLSFLATAVDGKPLGNGWLNLRLEAPRPIFLSTDYPLAEGSVLTEVRLPLQAGKA
                     EWKYNFPIRGNYRLFVEVLAPDGTQSGKMFAIEVGEHRSKWLTLAAFLVGLFGVGVLA
                     GRIFTHTVSKGTGALSAIFFASTWFALLSSTALAQTGVAKPIAQLEIDPPSAGKLAQI
                     RWIVDVDGENGGRRALLSLAITHIEKKMTVFAVDRIPVEGEFSMNFHFVDGAEHRVSA
                     RAELAGGRSIRSEQLVSVSAVEPPVSAMLPAMAVFLAAILFGLGVGRWSRCHAASS"
     gene            complement(3341..3529)
                     /locus_tag="VF089_15620"
     CDS             complement(3341..3529)
                     /locus_tag="VF089_15620"
                     /inference="COORDINATES: protein motif:HMM:NF021038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEX6175436.1"
                     /translation="MEKVFAGWSGATVPEPISFVMKSGMLLMLSGTILWIALAAHYPA
                     VHDAMHNFRHALAVVPCH"
     gene            3699..4715
                     /locus_tag="VF089_15625"
     CDS             3699..4715
                     /locus_tag="VF089_15625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175437.1"
                     /translation="MQFGVFDHLDRNNLPLQEYYESRLKLAEAYDRAGFYAYHVAEHH
                     STPLGMAPSPSVFLSAVAQRTHRLRVGPLVYALPLYHPMRLIEEICMLDQMSGGRLDI
                     GFGRGASQIESAMYGNDPAKAELMYVEGLELIMRGLTQKSLNFHGQFYSFDDVPMELE
                     PLQKPHPPIWYGVHSTVSAERAARQRLNIVSLDPADKTRTFTDRFRQVWRDTEDEETT
                     EPLIGLGRFIVVAASDEQAIDIARRAYPKWHRSFNYLFNLRGGKGPVHQRPADLETLI
                     KIGQGIAGNPETVTEFLKAQMTKSGANYVVGQFAFGDLSLSEILSSLELFTRHVMPEL
                     RTID"
     gene            complement(4749..5417)
                     /locus_tag="VF089_15630"
     CDS             complement(4749..5417)
                     /locus_tag="VF089_15630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6175438.1"
                     /translation="MGQMITFKRPDGAECNGYLATEAGNENAPGLVVIQEWWGLNDQI
                     KAVADRCAAQGYRALVPDLYKGKVTLDAAEAKHMMTNLNFADAAGQDIRGAAQHLKQS
                     SKKVGVVGFCMGGALTVLAAVFVNEADAASSWYGFPPEGAADVTKIKIPLQLHLAEKD
                     ASFTPEAARALEAKLKGANVPFESYWYDAGHAFGNEKGQNYNKEAADLAWERTSQFFT
                     KHLK"
     gene            5527..6042
                     /locus_tag="VF089_15635"
     CDS             5527..6042
                     /locus_tag="VF089_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175439.1"
                     /translation="MMAIKRAVLLRHVATAGILTIGFLPFCNLAAAQDVTDEIRQEQT
                     KVRQQQRLEELHRQEQIKQLQRDQKINEQQQQLDRLQRQNPRSDTLGKQDASEVQQQL
                     DQQRLEQQLQRLQNERQIDRAQREQDQLRQQQRIDQLQREGQIDHLRNQLRRNQVQQD
                     LDRLRQRQLNR"
     gene            6483..>6815
                     /locus_tag="VF089_15640"
     CDS             6483..>6815
                     /locus_tag="VF089_15640"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6175440.1"
                     /translation="METIGFVGTGTMGTALLSRLKLAHLCVTAFDIVPEALEQAKALG
                     AEPAASAKAVAQRSTLIDVFVRTDQEVLDCTLGNEGILEGASPGALVLLHSTIRPATT
                     KQVAHAAAD"
BASE COUNT         1663 a         1849 c         1786 g         1517 t
ORIGIN      
        1 aagaagaaga ccgtgggcaa tggcatgagc aaggaaaccc gtatgggccc gattcacctc
       61 gaatcgcagc gaaaggaagt cgaagagcaa gtagaagacg ctaaatcgcg cggcgctaag
      121 actttgttcg gcggggagcg acctaaaggt ggcgacctcg ataagggctt ctactatacg
      181 ccaaccctga tgagcaacgt gccggaggac tcccgcatga ccaccgaaga ggtcttcggg
      241 ccggcgctgc cggtatatac gtttaaaact ctcgacgaag cgctggaaaa agccaactcg
      301 tcggaatttg ggcttggttc ctcgatctgg acgcgcaatc ttttaagcgc caataaagcg
      361 attgataaat tgcaggccgg aaatgtctgg gtgaactcgc tgcactacgg ctacgacgaa
      421 ctccccttcg gtggagtcaa gtcgagcggc ctcggccgcg agcacggtcc cgaagcgctc
      481 gactattatt tcgagccaaa aggtgttgtc atcgttaatg tctgaccgat aacccggcga
      541 gtgggagccg gaaattttcc cgggtcccac tcatcttgtg catagccccg gcccgagccg
      601 cattcaaaac acaacttcca cgccgctgaa ctctgccgat ccctaccctc acttgtagcg
      661 ggcaagatcg ttggtagaat tgctagcggc ggttccacat gaccagcgaa aacgatgagg
      721 aatacttcgc ttgcctgaag gtttccccca ctcagatgga ttttctttgg gctgaggggt
      781 ggcggcactt cggcatttac ttctttcggt atcagacctc gtttcacgga gataagaagt
      841 tctcggttat gccgttgagg attgacctgg gacgattcag cttgacgcgc agtcaaaagc
      901 gcgtgctggc aaagaaccgg gatgcacgga ttatcatacg gccggctact gtcaattccg
      961 gtaaacacga ccttttcaat aaacacaaag tccgcttcaa agataatatc cctacgtcgc
     1021 ttgaagatat cgtatcaccg atgcctgcct cggtgccctg cccaagtcgt gagctatgtg
     1081 tttacctggg ggaggagctg gtgggtgtca cttacttgga tctcggacag gatgctactt
     1141 ccgctgttta cgcgatcttc gaccccgcgg aggcaaagcg aagcttaggg attctaatga
     1201 tccttcactc aattcagttt tcccgagagc gccgctgtcg atactattat ccgggttacg
     1261 cttaccagga accattcgcc tacgattaca aaaagagatt tacaggactg cagtatttag
     1321 attgggattc ggggtggcga gattacgcgg accacggcaa ttccgaggag gcactgtgag
     1381 cgtctttaaa cgaccggagg tacttcacag taggtttatg ttgcgatcgc catctacccc
     1441 caggtattaa gtctgtaaga tgcggttgac aatccgttcg ccgttcgatt aggttagcct
     1501 catctcgtcc aaaaggagcc cgcctatgtt cgcacagatg aagcacatgg ccatcgttag
     1561 cagcaattgc aaccaggagg gtgatttcta tcgcgatgtt ttcggcctga aacgctcggg
     1621 gctggctcgc gccggcggtg ctgttgtggt tcgcgatggt tacgtcgggc tcaacctcaa
     1681 cccccgcgct cctggccgcc aggccggttt tgaccatttt ggttttgagg tggaaaatgt
     1741 cgagaccgtc ttcgcccgag tcaatgagaa atacccaaag ataaaaattt taagacgtcc
     1801 tggcaatcgt ccatttgccg gcatcagttt gcacgatccg gcaggtaatg ttttcgacct
     1861 gtcacaaaaa gggatggaga atcgccgcga tctctatgtc gacgacgatg aaactatgcc
     1921 gcgccgggtc agtcacatcg ctttgcgcgt tctcgagccg gacttgattg ccgagttcta
     1981 tcgcacggtc ttcgagctca tggaattgga aaagccggct ggagatccca atcattacct
     2041 gtcagacggc cgagtcacgt tggcgatctt cccatggcag atcacccact atgaagggtc
     2101 cggtattgag cggccggctt tggaccacat aggcttcaaa gttgaaagta tgcaggcctt
     2161 caaagccgat ttggacaggt acatggccgc taatgctcct accgtcgctg atcgtcccga
     2221 gaaaaatcct gaaagagaag tcaggcgtaa tctattggcc aagtgctgct tcggtcaatt
     2281 tgagatgtca gatcccgatg gtgtgttgat tgatgtttca gaagcgtaaa ccaaccccta
     2341 gcggcgataa atcaggatga ggccgcatga cagcggctcc atcttcccac acctaacccg
     2401 aacagtattg ccgccaaaaa aactgccatc gccggcagca tcgccgagac tggtggctcc
     2461 actgccgaga ccgatacgag ttgttcggaa cggatcgatc tcccacctgc tagttccgcg
     2521 cgcgcgctaa cccggtgttc ggcgccatcg acgaaatgaa agttcatcga aaactcgcct
     2581 tcaacgggga ttctatcgac tgcgaataca gtcattttct tttcgatgtg agttatcgca
     2641 agcgacaaca gcgctctcct gccgccattt tcaccatcca catccacaat ccaacgaatc
     2701 tgggcaagct ttccggcaga gggcggatct atttccaatt gcgcgattgg cttagccact
     2761 ccagtttgag caagtgctgt tgacgatagt aatgcaaacc atgtgctggc gaaaaaaatc
     2821 gccgacaaag ctccggtacc cttactcacc gtatgcgtaa agatcctgcc ggcaaggacg
     2881 ccaaccccaa acagtcctac caggaaagcc gccaatgtta gccatttgct tctgtgctca
     2941 ccaacttcga tagcgaacat cttacccgac tgggtaccgt ctggcgccag cacttcgaca
     3001 aatagacgat agttgccccg tatcgggaag ttatacttcc actcggcttt tcctgcttga
     3061 agcggcaggc gcacctcggt gagcacgcta ccttctgcta acggatagtc tgtcgacaaa
     3121 aagattggtc gcggcgcttc gagccgcagg tttaaccatc cgttgcccaa cggcttgccg
     3181 tcgacggcag tggccaagaa cgaaagagtt gccggttcgg aaaacggccg caaacgttct
     3241 ggcactggcg aagttttgag atagatcttg aagttttggg ccgaaacgcc agcaccccag
     3301 agtatgcaaa agaagaaaag cgcgggggcg agaaaatacg tcagtgacaa ggaaccacgg
     3361 ctagagcatg ccgaaagtta tgcatcgcat catgaaccgc aggataatgg gccgcaagag
     3421 cgatccagag aattgtcccc gataacatca ataacatgcc cgatttcatg acaaacgaaa
     3481 tcggctccgg aactgttgcc ccggaccatc cggcaaatac tttctccata tggattcgct
     3541 cctttctcgt gactgtaatt taaatactag gactgaaaaa ctgtcaacct cagactgttc
     3601 gacactagcc ttgtaaggcc acaattgagc atataagatc cgcagaaatc tttaccatgt
     3661 aggaatcacc cgtcctcaga gtcggattcg gagcgcgcat gcaattcggt gtatttgatc
     3721 atctagaccg caacaatcta cccctccagg aatactacga atcacgcctc aagctagccg
     3781 aagcgtacga tcgggcgggt ttttacgctt atcatgtcgc cgagcatcac tctacgccgc
     3841 tgggcatggc gccttcgccg agtgtttttc tgtcggctgt ggcgcagcga acccaccggc
     3901 ttcgtgttgg cccgttggtt tacgcgctac cgctttatca tccgatgcga ttgatcgagg
     3961 aaatttgcat gctcgaccag atgagcggcg ggcgcctcga cattgggttt ggccgcggcg
     4021 cttcgcaaat tgagtccgcc atgtatggca acgatccggc aaaagccgag ctcatgtacg
     4081 tcgaagggtt ggagctcata atgcgcgggc ttacgcagaa gagcctcaac tttcatggtc
     4141 aattctattc cttcgacgat gtgccgatgg agctagaacc actgcaaaag cctcatccac
     4201 ccatctggta cggggttcac tccacagtga gcgccgagcg cgcagcgcgc cagcggctca
     4261 acattgtcag tctcgatcca gccgacaaaa cgcgaacctt tacagatcga tttcgccagg
     4321 tctggcgcga cactgaagat gaagaaacca ctgaaccgct aatcggcctc ggccgtttta
     4381 tcgttgttgc cgcgagtgat gaacaagcaa tcgatatcgc gcggcgagca tatccaaaat
     4441 ggcaccgcag tttcaactat cttttcaact tgcgcggcgg caaaggcccg gtccaccagc
     4501 gacccgcgga tctagaaacc ctaatcaaaa tagggcaagg cattgccggc aacccagaga
     4561 ctgtaaccga gtttctcaag gcgcagatga cgaaatccgg cgctaattat gtcgtcggtc
     4621 agtttgcgtt cggcgatcta tctctaagtg aaatactatc ttctctggaa cttttcactc
     4681 gccatgtcat gcctgagttg cgcacgatcg actgagatga aaggagaacc agctaccggc
     4741 ctagacgatc acttcagatg ttttgtgaaa aattggcttg tgcgctccca ggcaagatcg
     4801 gcggcctcct tattgtagtt ctggcctttc tcgttaccaa aggcatggcc ggcatcatac
     4861 cagtaggact cgaacggcac gttagctccc ttcaattttg cttcgagggc gcgcgccgct
     4921 tcgggcgtaa aagaggcgtc cttttccgcc aagtggagtt gcaaaggaat tttgatcttc
     4981 gttacatccg ctgctccttc gggaggaaag ccgtaccatg aagaggcagc gtcggcttca
     5041 ttgacgaaaa ccgcagccag cacagtcaga gcgccgccca tgcagaaacc tacgacaccg
     5101 acctttttac tggactgttt caggtgctgc gctgcgccgc ggatgtcctg cccagccgca
     5161 tcggcaaaat tgaggttcgt catcatgtgt tttgcttctg ccgcgtccaa agtcaccttt
     5221 cccttgtaga ggtccggaac gagcgcgcga tatccttgcg ccgcgcagcg gtccgccacc
     5281 gctttgatct gatcgttgag gccccaccat tcttggatca ccaccaagcc cggggcgttt
     5341 tcgttgccgg cctcagtggc aaggtaaccg ttgcattcgg cgccatcggg tcgcttaaac
     5401 gtaatcatct gtcccatgag attgcctcct tcttgacagc gaacttcttc cgttactatc
     5461 aaagggatcg atgaagtcaa tgcgcgcgct ctataacaac atgggttgga gtgttaaact
     5521 gattgcatga tggcgatcaa aagagccgtg ctcctacgcc atgtagcgac ggctgggatt
     5581 ctaacaattg gttttctacc gttttgcaac cttgctgcgg ctcaggacgt gactgatgag
     5641 atccgccagg agcagaccaa ggtccgtcag cagcaacggc ttgaggagtt gcatcgtcaa
     5701 gagcagatca aacagctgca gcgagatcag aaaatcaatg agcaacagca gcagttagat
     5761 cgattacagc ggcaaaaccc gcgctcggac actcttggga agcaagatgc ctccgaggtc
     5821 caacaacaat tggatcagca gcgacttgag cagcagctac agcggctaca gaacgagagg
     5881 cagatcgatc gggctcaaag agaacaggac caattgcgtc agcaacaacg gatcgatcaa
     5941 cttcagcgcg agggacaaat cgatcatctg cgcaaccaat tacggagaaa tcaagtgcag
     6001 caggatctcg atcgcctaag gcaacgacaa ctcaatcgct gaaagctata tccgagttca
     6061 acctaccgca aagtgcgtag tcgcgagcgc gaacacccaa gccccaagtt aagtcgactc
     6121 atcgtcaggt ataaacactt caacgaatgc gaggggtctg cctaatcagt ttgtcttctg
     6181 ccttataccg ttacgcttca caattgaccg tacagtagta tggtagcagc acccagctct
     6241 ggaagcggca gtgcgcgcgt tttgcttctc gcaaagtgca tacgagggga agagcatgct
     6301 taaagaactt gtccactcat gcagcattag agaaccagct gcggtcgctt cggattttct
     6361 cgacgatgtt ctgatgatat ggacaggagc gctgagtatg cgaagctcgt cattgaaaca
     6421 ccgcaacagc gcagttttag agaaatacaa caaggcttgt gcaagccgcg taaggagaaa
     6481 cgatggaaac aattggtttc gtaggtaccg gcactatggg aaccgccctg ctgtcgcgcc
     6541 taaagttagc gcacctgtgc gttactgcat tcgacatcgt gccggaggcg ctcgagcaag
     6601 caaaggcact cggcgctgaa cctgccgcat cggcaaaagc ggtcgcgcaa agatcgactc
     6661 tgatcgacgt tttcgttcgt acggatcagg aagtgcttga ttgtacgctg ggtaacgagg
     6721 gcatcctgga aggagcctcg ccaggcgcct tggtactgct acacagcacg attcgtcctg
     6781 ccacgacaaa gcaagtagcc cacgctgcag cggat
//
LOCUS       DASUTG010000677         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_698825, whole genome shotgun sequence.
ACCESSION   DASUTG010000677 DASUTG010000000
VERSION     DASUTG010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698825"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /gene="uvrA"
                     /locus_tag="VF089_15645"
     CDS             <1..1547
                     /gene="uvrA"
                     /locus_tag="VF089_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6175441.1"
                     /translation="ENGSSERDRAVADKIVKEIQQRLTFLSEVGLPYLTLDRRADTLS
                     GGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDNDMLLRTLRRLERLGNSVLVVEH
                     DEATIRSADLIVDLGPGAGVHGGNVVSIGSPDEIRDNPDSPTGAYLRSVKRRLGPQRD
                     LGKADWLTIRGAKAHNLKNIDVKIPLGMWSCITGISGSGKSTLVREVLYKGVKLLLGQ
                     FAGRPGAHKEIAGWKSIERIVEVDQTPIGKTPRSVPASYVGLLDEIRKLYALSPEARL
                     RGYTPSRFSFNVKGGRCEACAGQGKIRKEMSFLPDVFVDCDDCRGERFNEETLNIRFN
                     GRNIADVFHMTVEEAVPFFQAFPKIARPLKVLDDIGMGYITLGQASNTLSGGEAQRIK
                     LAYELGKDSRGKTLYLLDEPTTGLHFADVDKLIQILHRLVDRGNTVITIEHNLDIVKD
                     ADYLIDLGPGGGEQGGRLVAAGTPLQVSKDGKHSYTARFLREYLNGGSEAAEAVSPPI
                     LEQVIA"
     gene            1549..1941
                     /locus_tag="VF089_15650"
     CDS             1549..1941
                     /locus_tag="VF089_15650"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX6175442.1"
                     /translation="MARRILPMLGYGLRLKCPRCGVGSLYAAVFRMHTTCPHCGLIFE
                     REQGYFIGAIYINYAATVLIAVPGFFLLSIFTNLTIDQQLWIWISFAIIFPLLFFHHS
                     RSLWLVLDHIFNPPEKFYSVPPKNRQQS"
     gene            complement(2166..>2763)
                     /locus_tag="VF089_15655"
     CDS             complement(2166..>2763)
                     /locus_tag="VF089_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6175443.1"
                     /translation="DLSLVYPLARGTGPLLSTIAAVLLLGERPSALALIGALLIIGGV
                     VVITSNPKSLRQSGVKTAVLYAAVTGLFIAAYTLWDKQAVSRFAVPPLILDWGSNLVR
                     AIFLTSLAARYPQETRVEWREHRFEAIGIAVLAPLSYILVLTAMQFTPVSYVAPAREI
                     SILIGTAMGTRLLAEGEGRRRLGAASAMVLGVVALAVG"
BASE COUNT          604 a          812 c          772 g          575 t
ORIGIN      
        1 ccgaaaatgg cagcagcgaa cgcgatcgcg ccgttgccga taagatcgtc aaagagatcc
       61 agcaacggct gacttttctc tccgaagtcg gcctccctta tttaacgctt gatcgccgcg
      121 ccgacactct ttcgggcggc gaagctcaac gcatccgttt ggcggcgcaa ctcggctcga
      181 acctgcgcgg cgtctgttac attctggacg agcccacgat cggtcttcac ccgcgcgaca
      241 acgacatgct tttacgcacg ctcagacgcc tcgagcgcct cggtaacagc gtgctggtgg
      301 tcgagcatga tgaagcgacg atccggtcgg ccgacctgat tgtcgatttg ggaccgggcg
      361 ccggcgttca tggaggcaat gttgtcagca tcggatcgcc ggatgaaatc agagataatc
      421 ccgattctcc caccggggct tatcttagat cggtcaagag acggctcggc ccgcaacggg
      481 atctcggcaa ggctgactgg ctcaccatcc gcggcgccaa agcgcataac cttaaaaaca
      541 tcgatgtcaa aatcccgctg ggcatgtgga gctgcatcac gggcatttcc ggttcgggca
      601 aaagcacatt ggtccgagag gttctctaca agggagtcaa gttgctgttg ggccaatttg
      661 ccggccgtcc gggcgcgcac aaagagatcg ccggctggaa atccatcgaa cgaatcgttg
      721 aagtcgatca gacaccgatc gggaaaacgc cgcgctcggt gccggcctcc tatgtcggtt
      781 tgctggacga aatccgcaaa ctttatgcgc tgtcgccgga ggcgagactg cgcggttaca
      841 ccccgagccg gttctcgttc aacgtcaaag gcggacggtg cgaagcttgc gccggccagg
      901 gcaagatccg caaggagatg agttttctgc cggacgtctt tgtcgactgc gacgattgcc
      961 gcggcgaacg cttcaacgaa gagactttga acattcgttt caatgggcgg aacatcgccg
     1021 atgtttttca tatgactgtg gaagaggctg tgccgttttt ccaagccttt ccgaagatcg
     1081 cgcggccgct caaagttctc gatgacatcg gcatgggcta catcactctc ggacaggcga
     1141 gcaacactct gtccggcggc gaggcgcaac ggatcaaatt ggcctatgag cttggcaagg
     1201 attcgcgcgg taagacatta tacttactgg atgaacccac caccggcctg cactttgccg
     1261 acgtcgacaa gctgattcag attctccacc ggctggtcga ccgcggtaac accgtcatca
     1321 ccatcgagca caatctcgat atcgtcaaag acgccgacta tttgatcgat cttggccccg
     1381 gcggcggcga gcagggaggc cgcctggttg cggcgggcac gccgttgcaa gtcagcaaag
     1441 acggtaagca ttcatacacc gcccgttttt tgcgcgaata tctgaacggg ggctccgaag
     1501 ccgccgaggc agtttcgcca ccgattctcg agcaggtaat cgcttgacat ggcccggcgc
     1561 attctaccga tgctgggata tggtctgcgc ctcaaatgcc cgcgctgcgg cgtcggatct
     1621 ttgtatgcgg cggtgttccg gatgcatacg acctgtcctc attgcgggct catattcgaa
     1681 cgggagcagg gctactttat cggcgcgatc tacatcaatt acgccgccac cgtgctgatc
     1741 gcggtgccgg gattttttct cctgagcatc tttaccaacc tgaccatcga ccaacaactg
     1801 tggatttgga tctccttcgc gattatattt ccactactct ttttccatca ttccagaagc
     1861 ttatggttgg tgctggacca tatcttcaat ccgcccgaga agttttacag cgtgccgccg
     1921 aaaaaccgtc agcagagctg acggcttaac cttaggctgt tcaaaagttc aaccgttcaa
     1981 cggctcaaag tccgacgttg aacagcggtt caaaaactgc aaggttccaa tctgttcagt
     2041 tctttaagac caatgatgat gccttcaaag cctatcattg atcacgagat cggtgtcggc
     2101 acgtggcaat ccatcggagg ttctgacggt gaactattga accttcgaac tgttgaacaa
     2161 agtagctaac cgaccgccag cgctaccacc ccgaggacca ttgccgatgc ggcgcccaac
     2221 ctgcgccggc cttcaccttc cgcaagcagg cgggttccca tcgcggtgcc gatcagaatg
     2281 ctgatttcgc gcgccggcgc gacgtaactt accggggtaa actgcattgc ggtgagaacc
     2341 aggatgtagg agagcggtgc tagaacagca attccgatcg cttcaaaccg gtgctcgcgc
     2401 cattccactc gcgtttcctg aggatagcgc gccgcgagag atgtcaggaa aatcgctctg
     2461 acgagattcg agccccagtc aagtatcaga ggcggcaccg cgaaccggct caccgcctgc
     2521 ttgtcccaca gggtataagc cgcgatgaac aagccggtca ccgcagcgta gagcaccgcg
     2581 gttttgactc cgctctgacg caagcttttc gggttgctgg taatcacgac cacgccgccg
     2641 atgatcagca atgcgccgat cagcgccaac gccgaaggtc tctcgccgag tagcaagaca
     2701 gccgcgatcg tcgaaagcaa cggccctgtg ccgcgcgcca ggggatagac caacgagagg
     2761 tca
//
LOCUS       DASUTG010000678         6616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_698883, whole genome shotgun sequence.
ACCESSION   DASUTG010000678 DASUTG010000000
VERSION     DASUTG010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698883"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VF089_15660"
     CDS             <1..209
                     /locus_tag="VF089_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6175444.1"
                     /translation="HLKACGIAVPIGPHGLRHTFATHLLNSGADLRVIQEMLGHASLS
                     TTQHYTHLNLDQLTAVYDKAHPRA"
     gene            300..830
                     /gene="hslV"
                     /locus_tag="VF089_15665"
     CDS             300..830
                     /gene="hslV"
                     /locus_tag="VF089_15665"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX6175445.1"
                     /translation="MFEGTTILAIRHQGKVVVVGDGQVSLGQTVMKHGARKVRKLHHD
                     KIIAGFAGATADAFTLFEKFEAKLEQFNGNLKRAAVELAKDWRTDRILRRLEALLIIA
                     DINDLLVISGAGEVIEPDDGVIAIGSGGNFALAAARVLVKHSQLDARTIAEESMRAAA
                     SLCVYTNEQLTFEELS"
     gene            987..2318
                     /gene="hslU"
                     /locus_tag="VF089_15670"
     CDS             987..2318
                     /gene="hslU"
                     /locus_tag="VF089_15670"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX6175446.1"
                     /translation="MTPREIVSELDRYIVGQRDAKRAVAIALRNRWRRQQVSEDLREE
                     IVPKNIIMIGPTGVGKTEISRRLAKLAQAPFIKVEASKFTEVGYVGRDVESIIRDLTD
                     LAVNMVKEEEKQKVEIKAHEVAEERVLDLLLPGGPGAGMDGEAIDSARVQGTREKLKK
                     MLREGKMDERFVDIEVTQSAMPMIEVLTPQGMEGMEFNLKEMFSNLMPKKTKKKTVKI
                     PEALQILTQEEAGKLVDMDKVSSEAVRRVEQSGIVFLDEIDKIAGREAGQGPDVSRQG
                     VQRDLLPIVEGSTVNTKYGMVKTDHILFVASGAFHTAKPSDLIPEFQGRFPIRVELSS
                     LGKEDFIRILTEPKNALIKQYEALLATERVHLKFGAGAIEEIARIASDVNDRMENIGA
                     RRLHTILERLLDDVSFAAPEMQGKEVAIDAEYVRERLTPILKNEDLSRYIL"
     gene            2328..3212
                     /gene="argB"
                     /locus_tag="VF089_15675"
     CDS             2328..3212
                     /gene="argB"
                     /locus_tag="VF089_15675"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX6175447.1"
                     /translation="MDDFIGKAEVLMEALPYIQRFYGKTFVIKYGGHAMENEELKQSF
                     AQDIVLLKYVGINPVVVHGGGPQIDTVLEKMGITSRYVRGMRVTDQATLDIVEMVLVG
                     KVNKEIVNLINRHGGLAVGLSGKDGGLILARKMNLTVSSPNGAPPEIIDIGMVGEIIG
                     INPLVIESLDQNKFIPVIAPVGVGQEGETYNINADLVAGEVAEALHAEKLIMMTDVEG
                     VKDKRGSLLSTLKENQARKMIQDGVVGSGMIPKVECCVNALKGGVGKTHIVDGRVKHA
                     VLLEIFTKEGIGTEVVRK"
     gene            3213..4400
                     /locus_tag="VF089_15680"
     CDS             3213..4400
                     /locus_tag="VF089_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6175448.1"
                     /translation="MNNLEIAALTDKYVARTYARIPIALVRGKGTRVWDADGKEYLDF
                     LAGIAVNNLGHCHPAILRAIREQSKKLLHVSNLYHILPQSELARELCHHSFAERVFFC
                     NSGAEANEAAIKLVRRYGLEKLGGRYEILSTHNSFHGRTLATLTATGQEKIRAGYDPL
                     PSGFRQVPYNDLAAIEEAIDEKKTAAVLVEPIQAEGGVNVPDEAYMRGLRELCDQRSI
                     LLIFDEVQTGMGRTGRLFGYEHFAIKPDIMTLAKALGGGLPLGAMLAREEIATSFGPG
                     SHASTFGGNPVACSAGLVVMQTLLSGALENCLQMGSYFVRGLEALQKRFSFIREVRGK
                     GLMIGMELEIEGSKIADSCMHEGLLLNCTAYKVLRFVPPLTIRKNEIDRGLDILEKVL
                     ARQ"
     gene            4424..5425
                     /gene="argF"
                     /locus_tag="VF089_15685"
     CDS             4424..5425
                     /gene="argF"
                     /locus_tag="VF089_15685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6175449.1"
                     /translation="MSKRDLLNFADWSREELDSILRLARDLKQKQKRGLIHRLLEGKG
                     LGMLFEKPSLRTRVTFEAGMVQLGGYAVFLSPTEVQMGVRETPEDCARSLSRWVDMIV
                     VRTFAQATLEEMARAASVPVINALTDLYHPCQVLADCLTLLEHKGKLEGLKVAFVGDG
                     NNMVHSWMEAAEKIPFSFALACPKGYEPSRDITARVKRNGARVTVTHSIRDALNGADA
                     IYTDVWTSMGQEWDVATRLKAFHEYQVNYRVLAMAKKDAVVMHCLPAHRGQEITDEVL
                     ESPQCIAFDQAENRLHAQKAIMVWLLDGAKSLTAQTSKGSRRRANQTTRSRGSNKKR"
     gene            5422..>6616
                     /locus_tag="VF089_15690"
     CDS             5422..>6616
                     /locus_tag="VF089_15690"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX6175450.1"
                     /translation="MNVKKVVLAYSGGLDTSVILRWIKEEFGCEVIAFCADLGQGEDL
                     QAIKRKAIDTGASKAVIGDLREEFVKNYVFPMLRGNAIYEGSYLLGTSIARPLIAKAQ
                     IDVAAKERADAVSHGATGKGNDQVRFELTYYALKPAIKVIAPWRLWSLNSRTSLIAYA
                     RKHGIPVPVTKARPYSTDRNLFHISYEGGILEDPWKEPPADMFVWCRPLEKAPNRAEY
                     VEIDYVRGDPVAINGKRLSAAKLLGQLNDLGAKHGIGRIDLVENRYVGMKSRGVYETP
                     GGTVLHAAHRALESITMDREVMRLRDSLIPRYAEMIYYGYWFSPEREVLQGTIDAAQR
                     NVNGTARLKLYKGNVVVAGRKSDSSLYDSKVATFEQDTVYQQADAEGFIRLNALRLRL
                     QKLSKT"
BASE COUNT         1598 a         1689 c         1930 g         1399 t
ORIGIN      
        1 agcatttgaa ggcttgcggg attgctgtgc ctatcggtcc gcacggtttg cggcacacat
       61 ttgccactca tcttctaaat agcggcgccg atcttcgagt gatacaggaa atgctcggcc
      121 atgccagcct ttccacgacc cagcactata cgcacctgaa cttggatcaa ttaacggcgg
      181 tatacgataa agctcatcct cgggcttgaa acagggctta ggagatcgcg agtgccgtgc
      241 gagggtatct acaatcgcgg gttcattgaa atcgggattc taccttgtga gttgaggcca
      301 tgtttgaagg aacaacaata ttagcgattc ggcaccaggg aaaagtggtg gtcgtcggcg
      361 atgggcaggt gagtctcggc caaaccgtga tgaagcacgg cgcgcggaag gtccgcaagc
      421 tccatcacga caaaatcatc gctggttttg ccggcgcaac agccgacgca tttaccttat
      481 tcgagaagtt tgaagccaag ctggaacagt ttaacggcaa tctcaagcgt gcggcagtgg
      541 agctggccaa ggactggcgc accgaccgaa tcttgcggcg gcttgaggcg ctacttatca
      601 ttgccgacat caatgatctg ttggttattt ccggggcggg cgaggtgata gaacccgacg
      661 atggcgtcat tgccatcggt tccggaggaa atttcgcatt ggcagcagct cgggttctgg
      721 tgaagcacag ccagcttgac gcgcgcacga ttgctgaaga atcgatgcgc gcggccgcca
      781 gtctctgcgt ctataccaac gagcagctta cgtttgaaga gctgtcgtaa acgtgagccc
      841 cttcgataga tgcaggaaac cgtagctttg tgcaactaaa gagtatttgc ttttgtcttt
      901 gaagttgcta ggagttagct attctaatga agcatgaacc gatcatagcc ctgccggatt
      961 cgtctgactc ttccccggcg ccagtgatga cgccacggga aatcgtttct gagctcgacc
     1021 gttacattgt cggacaaaga gacgccaagc gcgccgtggc tatcgcactg cgcaatcgct
     1081 ggcggcgcca gcaggtttca gaagatctgc gcgaagagat cgtacctaaa aatatcatca
     1141 tgattggccc caccggagtg ggtaaaaccg agatctcgcg ccggcttgcg aagctcgcac
     1201 aggcgccgtt catcaaagtc gaagcctcca aattcactga agtcggctat gtcggccggg
     1261 acgtcgagtc gatcatccgc gatctcaccg atcttgccgt taacatggta aaagaagaag
     1321 agaagcagaa ggttgaaatc aaagcccatg aggttgccga agagcgcgtt ttggatttgc
     1381 tattgcccgg tgggccgggg gcgggcatgg acggagaggc aattgatagc gcacgggtgc
     1441 agggcacacg cgagaagctg aagaagatgc ttcgtgaagg caagatggat gagcgcttcg
     1501 tagacattga agtcacccag tcagcgatgc cgatgatcga agtgctgacg cctcaaggca
     1561 tggaaggcat ggaatttaac ctgaaggaaa tgttttccaa cctgatgccg aagaaaacca
     1621 agaaaaaaac ggtcaagatt cccgaagccc tccagatcct tacgcaggaa gaagcgggaa
     1681 aattggtcga catggacaag gtgagcagcg aagcggtgcg ccgggtggag cagtctggaa
     1741 ttgtctttct cgatgaaatc gataaaatcg ccggccgcga agcgggccag ggacccgatg
     1801 tttcccgcca aggcgtacaa cgggatttgc tgccaatcgt tgaaggttcg acagtcaata
     1861 ccaaatacgg catggtcaag accgatcaca ttttgtttgt cgcttcaggg gcgtttcata
     1921 ccgcaaagcc ttctgatttg atccccgaat tccaaggccg atttccaatt cgcgtagagc
     1981 ttagttcttt gggcaaggaa gacttcatcc gtatcctaac cgaacccaag aacgcgctga
     2041 tcaagcaata cgaagctctg ctggcaacgg agagagttca cctaaagttc ggcgccggag
     2101 ccatcgaaga aatagccagg atcgcttccg atgtgaacga tcgaatggag aacatcggtg
     2161 cccgccgcct ccacaccatt ctcgaacgtt tgctcgacga tgtctctttc gccgcccccg
     2221 agatgcaggg caaggaagtt gccatcgacg ccgaatatgt gcgcgaacgg ctcacgccaa
     2281 tcttgaagaa cgaagatctg tcccgctaca tcctgtgatc taagcgcatg gacgatttca
     2341 tcggcaaggc agaggtgttg atggaagcgc tgccttatat tcagcgcttc tacggcaaga
     2401 cttttgtcat taaatacggc ggccacgcga tggaaaacga ggagctgaag cagagcttcg
     2461 ctcaagacat cgtgctgctg aagtacgttg gcatcaatcc cgtcgtggtg cacggcggcg
     2521 gcccgcaaat cgatacggtg ctcgagaaga tggggatcac cagccgttac gtgcgcggca
     2581 tgcgtgtcac ggaccaggca actttggaca tcgtcgaaat ggttctggtt ggcaaggtga
     2641 acaaggaaat agtaaacctt atcaatcgtc atggcggcct agccgtagga ttgagcggca
     2701 aagacggcgg tttgattctg gcgcgcaaga tgaatcttac cgtttcctcg ccgaacggcg
     2761 caccgccgga aatcatcgat atcgggatgg tgggcgaaat catcggcatc aacccgctcg
     2821 tgatcgagtc gctggaccag aacaaattca ttccggttat cgctcccgtt ggcgtcgggc
     2881 aggagggcga gacctacaat atcaacgccg acctggtggc cggcgaagtc gccgaagctc
     2941 ttcacgccga aaagctcatc atgatgaccg acgtcgaggg agtaaaagac aagcggggca
     3001 gcttattgag cacgctaaag gaaaatcaag cgcgcaagat gattcaggac ggagtggttg
     3061 gctctggaat gattcccaag gtcgaatgct gtgtcaatgc gctgaaaggc ggagtcggca
     3121 aaacccacat cgtagacggc cgggtaaaac acgcggtact cctcgaaatc tttaccaaag
     3181 agggaattgg cactgaggtg gtgcgcaaat aaatgaacaa cttggaaatt gctgcgctga
     3241 ctgacaaata tgttgctcga acctacgctc gtatcccaat tgctttagtc aggggaaaag
     3301 gcactcgggt ctgggatgcc gacggcaaag agtacctcga ctttctggct ggaatcgcgg
     3361 tcaacaactt gggtcactgc cacccagcta tcctccgggc catccgggag cagtcgaaaa
     3421 agctgctgca cgtttcgaat ctctatcaca tcctgccgca atcggagtta gcgcgcgagc
     3481 tgtgccacca ttcgtttgcc gagcgggttt tcttttgcaa cagcggcgcc gaggcaaatg
     3541 aagcggcgat caagctggtt cgacgctacg gtctcgagaa gctcggaggt aggtacgaga
     3601 ttctttcaac acataactcc tttcacggcc gcacactcgc aaccctgacc gccacggggc
     3661 aggagaagat ccgcgcgggt tacgatcctt tgcccagcgg gtttcgccag gtgccctaca
     3721 atgatttggc tgccatcgaa gaagccatcg acgagaagaa aaccgccgcc gttttggttg
     3781 agccgatcca ggctgagggt ggggtcaacg tcccagatga agcttacatg cgcggcttgc
     3841 gtgagctctg cgaccaacgc agcatcttgc tcatcttcga tgaagttcag acgggaatgg
     3901 ggcgcaccgg caggctgttc ggctatgagc atttcgctat caaaccggac atcatgactt
     3961 tggccaaggc tctcggggga ggcctgcctc tcggcgcgat gttggcgcgc gaagagattg
     4021 ccactagctt cggcccgggt agtcatgcgt cgacgtttgg cggtaatccg gtggcgtgca
     4081 gcgccggctt ggttgtgatg caaactctgt tgagtggcgc gctagagaac tgcctgcaga
     4141 tgggaagcta ttttgtccgc ggcctcgaag ctctgcaaaa gcgcttttct ttcatacgag
     4201 aagtgcgcgg caagggactg atgatcggca tggagctgga gatcgaaggc agcaaaatcg
     4261 ccgatagctg catgcacgag ggattacttc tcaactgtac cgcctacaaa gttctccgtt
     4321 ttgttccacc gctgacaatc aggaaaaatg aaatcgaccg gggcctcgac attctcgaaa
     4381 aggttctcgc ccgccaataa gccgctcgtc ggctgtccca gtcatgagta aacgagattt
     4441 attgaatttt gctgattgga gccgcgagga gctcgattcg attcttcgac tggctcgcga
     4501 tctgaagcag aagcagaaac gaggcctaat ccatcgcctg ctcgagggca aagggttagg
     4561 catgctgttt gagaaaccga gtttgagaac gcgggtgact ttcgaggccg gcatggtcca
     4621 gctcggcgga tatgcagtgt ttctctcgcc cacggaagta cagatgggcg tgcgcgaaac
     4681 tccggaagat tgcgcgcgca gcctgagccg ctgggttgac atgatcgtcg tgcgcacgtt
     4741 cgcgcaggca accctggaag aaatggctcg tgctgcctcg gtgcctgtca tcaacgcttt
     4801 aaccgatctc tatcatccct gtcaggtcct tgccgactgc cttacgttgt tagaacacaa
     4861 gggcaagcta gaaggactaa aggttgcgtt cgttggtgac ggtaacaaca tggtacattc
     4921 ctggatggag gccgccgaga agatcccatt ttcgttcgcg ctggcatgtc cgaagggcta
     4981 cgaaccgagc agagacatca ctgctcgtgt aaaaagaaat ggcgctcgcg taactgtaac
     5041 ccattcgatt agagatgctt tgaacggagc cgatgcgatc tacaccgatg tctggaccag
     5101 catgggacag gagtgggacg tggccacccg gctcaaagct ttccatgaat atcaggtgaa
     5161 ttatcgagtt ctggcgatgg ccaagaaaga tgcggtggtt atgcactgct taccagccca
     5221 ccggggccag gaaatcactg acgaggtgtt agagagtccg cagtgcatcg ccttcgatca
     5281 ggctgaaaac agattgcatg cgcaaaaggc catcatggtt tggttgctcg atggcgcaaa
     5341 aagtttaacg gctcaaacct ccaaaggttc aaggagaagg gcgaatcaaa ctacgagaag
     5401 cagaggttcc aacaaaaaaa gatgaacgtc aagaaggtgg tgctggctta ttcgggagga
     5461 ttagacacat cggttattct gcgttggatt aaagaagaat ttggctgcga agtcatagct
     5521 ttctgcgccg atctaggaca gggggaagac ctgcaggcga tcaaacggaa ggcgatcgat
     5581 accggggcaa gcaaagcggt aatcggcgac ctgcgggaag agttcgtgaa aaactatgtc
     5641 ttcccgatgc tgcgcggcaa cgcgatctac gaaggttcct acctgctcgg cacctccatc
     5701 gcccggccgc tcatcgccaa ggcgcagatc gacgtcgccg caaaggagcg cgccgatgcg
     5761 gtatcgcacg gcgccaccgg caagggcaac gatcaggtgc gttttgagtt gacgtattat
     5821 gcgctgaaac cagccatcaa ggtgatcgcg ccgtggcggc tttggagtct caattcacgc
     5881 acctctttga tcgcctatgc gcgcaagcat ggcattccgg tgccggtgac caaggcgcgt
     5941 ccgtacagca ccgatcgaaa tctctttcac ataagttacg agggcggcat tctcgaggac
     6001 ccctggaaag agccgccggc agacatgttt gtctggtgcc ggcctttgga aaaggcgccc
     6061 aatcgagccg agtatgtcga gattgattac gtgcgcggcg atccggttgc gatcaacggc
     6121 aagagattgt ctgccgccaa acttctgggg cagttaaacg atcttggcgc caagcatgga
     6181 attggccgca tcgatcttgt cgaaaatcgc tatgtcggca tgaaatcacg cggtgtctat
     6241 gagacacccg gcggcaccgt attgcatgcg gctcatcgtg cgctggagtc catcaccatg
     6301 gatcgcgagg tgatgcggct gagagattcc ttgattccgc gctacgctga aatgatctat
     6361 tacggttact ggttctcgcc ggaacgtgag gtcttgcagg ggacaatcga cgcggcgcag
     6421 cgcaatgtaa acggaacagc gcggttgaag ctttacaagg gcaatgtcgt tgttgccggg
     6481 cgtaagtccg attcttcgct ctacgattcc aaagtggcga cttttgagca agataccgtt
     6541 tatcaacagg cggacgccga gggattcatc cgcttgaatg ctctgcggtt gcgtttgcag
     6601 aagctgagca aaacca
//
LOCUS       DASUTG010000679         6717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_401549, whole genome shotgun sequence.
ACCESSION   DASUTG010000679 DASUTG010000000
VERSION     DASUTG010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401549"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VF089_15695"
     CDS             complement(<1..1926)
                     /locus_tag="VF089_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX6175451.1"
                     /translation="MTDTEKTLRELLAQRILILDGAMGTMIQSHKLDEAAFRGRRFAN
                     HCRDLRGCNDVLCLTQSDLIAEIHFQYLEAGADIVETNTFNSTSVSMADYKLEADVYD
                     INVAGSRAAKKAVEAIMAKDPCRPRFVAGAIGPTNRTCSISTDVHSAATRGVTYEELV
                     QTYYDQARGLVDGGADILLVETIFDTLNAKAAFFAIATLFDERQLELPLMASVTFIQP
                     GSNRGVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIEELSRIAPVYVSTHPNA
                     GLPDPLLPTGFPETPQSLAPQLREWAENGWLNIVGGCCGTTPAHITSIAEAVHGLPPR
                     IPPVPEPYLRLSGLESVTIRPESNFVNIGERTNVTGSPVFAKLILAGDYEKAVSVARQ
                     QVEGGAQIIDVNMDEGMLDSKAAMEKFLRLIAGESEIARVPVMVDSSRWEVIEAGLRC
                     LQGKGIVNSLSMKEGEEKFIAQAKRVRRYGAALIVMAFDERGQADTYERKIQVCARSY
                     KILTEQVGFPPQDIIFDPNILTVGTGIEEHNSYAVNFIEATRWIKENLPLAKVSGGVS
                     NISFSFRGNNPVREAMHSAFLYHAIKAGMDMGIVNPGMLAVYEEIPKDLLELVEDVLL
                     NRRSDAIERLVTFAETYK"
     gene            complement(1923..2906)
                     /locus_tag="VF089_15700"
     CDS             complement(1923..2906)
                     /locus_tag="VF089_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6175452.1"
                     /translation="MDIVRTIKALGDPLRLRVLAAVTQEELTVGEVQEVVGSVQSSVS
                     RNLAILREAGFIQDRKEGTNVYFSARRDMPAPAQELFKSVRERIKDLPEAKGDRARLE
                     NCRRRRLSRSRNYFETVAGDWERIRRSYFDDRVASLAIEKLLPRNLVLADVGCGTGTL
                     TFELARFARKVIGIDLSQEMLRRARELAKEKKLANVELRFGDALKLPITTHSVDAAFC
                     VMVLHFLPDPQKAIAELCRIVRPGGSIVLVDLVQHKQEWMRKQMAHRWLGFDRDAIED
                     SFRVAGAQGVDYELTGSYAGEKMARNGNQPVEIFVARASLTKISTPRKAKR"
     gene            complement(3358..3939)
                     /locus_tag="VF089_15705"
     CDS             complement(3358..3939)
                     /locus_tag="VF089_15705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175453.1"
                     /translation="MSGDDKTRWDRQHRVSSGADQPSSFLRQVLESDAWHIPRGLALD
                     VATGKGRNAIFLAEMGFSVVGIDISTIALEEARRRAAEKSLEIRWHLADLERIELPPA
                     AYNLIVNVNYLQRSLIHQIRRALKPQGHVTFETYLIDQQAIGHPKNPAYLLNHNELLD
                     HFRSFRVLCYREGKYIDTGEATFRAGIFAKKIR"
     gene            complement(4232..4999)
                     /locus_tag="VF089_15710"
     CDS             complement(4232..4999)
                     /locus_tag="VF089_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX6175454.1"
                     /translation="MVPGRDRSFAERFELMVDWLYPPRCRACSGSIQGRDYEYFCSTC
                     WPQIQLVAHPFCTTCGRPFPDSSGDDHACGVCIARNPHFARAHSWACYPREEATEHPL
                     RQVIQKFKYGRKVALGKPLGRLMARGCEEFLATCKAEAIIPVPLHPRRLRWRGFNQSV
                     LLARQIGRAYNLPLDCFTLYRKMDTPPQTQLPEEDRRKNMRGAFALAGESSVEDKRLL
                     LVDDVYTSGATVNECSRVLKGGGAREVFVLTLARAVY"
     gene            complement(5076..6632)
                     /gene="gpmI"
                     /locus_tag="VF089_15715"
     CDS             complement(5076..6632)
                     /gene="gpmI"
                     /locus_tag="VF089_15715"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX6175455.1"
                     /translation="MKGTTVATNQPVILVVLDGFGINPKREGNAIANASMPNMDSLLR
                     NYPTSSLSMSGLDVGLPDGQMGNSEVGHMILGAGRIVYQDLTLIHKDIDEGNFGKNQI
                     ISNALRTTKAEGGRLHLMGLLGDGGVHSHQRHMEALIEMAQREKVAPVYLHLFLDGRD
                     TPPNSAEQFMLDLNDKLKTWPDVKIATLTGRYYAMDRDKRWDRVEKAYLCLTEGAGKL
                     SDSPLEAIRNSYSEGVTDEFVLPAVIRSTISEGLIRDGDGVIFFNFRADRARELTRAF
                     IDADFKEFPRKHRLNLSTYTTMTQYDETFKVPVAYPPRELRKILGEVVSQHGLKQLRI
                     AETEKYAHVTYFFNGGEEKEFPGEQRILIPSPKDVPTYEFKPEMSAREVTEALVKKFS
                     EDHINLVIANFANADMVGHTGNFEASVKACEVIDECLGKVVDAALSRKARVLITADHG
                     NIEQLIDYDTGMPHTAHTINRVPIILVDEKRKQSRLREGTAIDVAPTVLHLLELPQPL
                     EMTGHSLITD"
BASE COUNT         1539 a         1849 c         1764 g         1565 t
ORIGIN      
        1 tttgtaagtt tcagcaaaag taactaggcg ctcgatggca tcggagcggc gattgagcaa
       61 gacatcttcg accaattcga gcaggtcctt gggtatttcc tcatagaccg ccagcatgcc
      121 cggattgacg attcccatat ccattccagc tttgatggcg tgatacaaaa atgccgaatg
      181 catggcttca cgaaccggat tgttgcctct gaaagaaaag gaaatattgc tcacgccgcc
      241 gctgactttg gccagcggca aattttcttt gatccatcta gtcgcttcaa tgaaattgac
      301 ggcgtagctg ttgtgttctt caataccggt gccaacagtt aatatatttg gatcgaagat
      361 gatgtcctga ggtggaaacc caacctgctc cgtcagaatc ttgtacgagc gggcgcaaac
      421 ctggatcttg cgttcgtagg tatcggcctg accccgttcg tcgaatgcca tgacgattaa
      481 cgccgcgccg tagcgacgaa cccgcttggc ctgcgcgata aacttttctt caccttcttt
      541 catgctgaga gaattgacga tgcctttgcc ctgtagacac ctaagcccgg cctcgatcac
      601 ctcccatctt gaactgtcga ccatcaccgg tacccgggcg atttccgatt cgccggcaat
      661 gagccggaga aacttctcca tggccgcttt ggaatcgagc atgccctcgt ccatattaac
      721 gtcgatgatc tgcgcaccgc cctcgacctg ttggcgcgca acgctgaccg ccttctcgta
      781 atcgcccgcc aagatgagct tggcgaacac cggcgatcca gtgacattgg tgcgttcgcc
      841 gatattaacg aagttagact ctggccggat ggtgacgctc tccaggccgc tcaagcgaag
      901 atacggctcg ggtacaggtg ggatgcgggg cgggagcccg tgcacggctt cggcaatact
      961 cgtgatgtgc gcgggcgtgg tgccacagca cccgccaaca atgtttagcc agccgttctc
     1021 cgcccattcg cgcagttgcg gcgccaagct ctgcggggtc tccggaaaac ccgtcggcaa
     1081 cagcggatcg ggtaggccgg cattggggtg ggtgctaacg taaacgggcg caattcgaga
     1141 taattcttcg attagcggac gcatctcttt gggccccaag gcgcagttca tgcctacgct
     1201 caacaacggt acgtgggaaa tggagttcca aaaagcttcg accgtttgac cggtgacgcc
     1261 acggttgctg ccaggctgaa taaaagtgac agaagccatc aacggcagct cgagctggcg
     1321 ctcatcaaat aaagtcgcga tggcaaagaa tgccgcttta gcattgaggg tgtcaaaaat
     1381 cgtttccacc agcaggatgt cggcgccccc gtcgaccaat ccccgggcct gatcataata
     1441 agtttgcacc aattcttcgt aggtcacccc gcgcgtcgcc gcgctgtgca cgtccgtcga
     1501 gattgaacag gtgcgattgg ttgggccgat agctcctgcg acaaaccgcg gccggcacgg
     1561 atctttagcc atgatcgctt cgacagcctt cttagccgcc cgggagcccg cgacgttgat
     1621 gtcgtaaaca tcggcttcga gtttgtaatc cgccatggaa actgaagtcg aattgaaggt
     1681 gttggtttca acgatatcag cgccggcttc cagatactgg aaatgaattt ccgcgatcaa
     1741 atcgctttga gtaagacaaa gcacgtcgtt acaacctctt agatcgcgac aatggtttgc
     1801 gaaacgtcgc ccgcgaaatg ccgcttcgtc gagtttatgg gactggatca tggtccccat
     1861 ggcgccatca aggatcagta ttctctgggc taatagctcg cgcaatgttt tctcagtatc
     1921 ggtcatctct tggccttcct cggggttgat atttttgtca agcttgcacg ggcgacgaaa
     1981 atttccaccg gctgattacc gttacgagcc attttttcac cggcataaga acctgtcagc
     2041 tcgtaatcga ctccttgcgc tccggcaaca cgaaatgaat cttcaatggc atcgcgatcg
     2101 aacccaagcc aacgatgcgc catctgcttg cgcatccatt cctgcttgtg ttgcaccaaa
     2161 tcgaccagga caatcgatcc acctggccgt acgatccggc atagttccgc aatcgctttt
     2221 tgtggatctg gcagaaaatg caataccatg acgcagaacg cggcatccac gctatgtgtt
     2281 gtaatcggaa gcttcaacgc atcgccaaaa cgcaactcga cgttcgctag ctttttttcc
     2341 ttagcaagtt cacgggcgcg tcttaacatc tcttgcgaga gatcgatacc gatcactttc
     2401 cgcgcaaatc gcgccagttc aaatgtcaga gtgcccgtgc cgcagccgac atcggccaac
     2461 accaggtttc gcggcaacag cttttcaatg gcgagggaag cgacccgatc gtcaaaatag
     2521 cttcttcgaa tcctctccca atcgccggcc acagtttcga aatagttgcg cgagcggctc
     2581 aagcgccggc gccggcaatt ttcaagccga gcgcgatcgc ctttggcttc cggcagatct
     2641 ttaatccgct cgcgtaccga cttgaataat tcctgggccg gggccggcat atcgcggcgg
     2701 gccgagaagt aaacgttagt gccttcctta cggtcctgta taaaacctgc ctcgcgcaga
     2761 atcgccagat tgcgcgacac ggacgactgc accgagccga cgacttcttg cacttcgccg
     2821 acggtcagct cttcctgggt aaccgcggca agaacgcgca agcgcaacgg atcacccaaa
     2881 gctttaatag tacgaacgat atccatgagg gcatctgaaa cagatatcgc aatatataga
     2941 ttattcgata ttcatgcaag ggaatggaat tcaaccggga acagagaacc gcattcgcga
     3001 ggggtaaaca tcagttgcag aatgaacgag gtccagaacg tcaagctatc ctgcgctccg
     3061 cgcggcatgg tgggagataa acagctcggt atagagagcg ggccagagca gacatttcat
     3121 gcgctgagaa catcgaaaga atccacgtca tggatcatga aaaaattctt atgctcaatg
     3181 agcgatcgca tgcttatcgc ggccgggata gacttgttaa ccgggctgac gatccatcgt
     3241 acgatcggag atttaggcgc gatgccggca cgctgtaaag gtattcgcgc tggcgttagt
     3301 ctggttagcg gttgtttgct cttccctgcc actccgatcg agaactggca agccacctta
     3361 tcgaatcttc ttagcgaaaa tcccggcgcg aaatgtcgcc tctccggtgt cgatgtactt
     3421 accttcccga taacagagaa cccggaagct acggaagtga tccaacaatt cgttgtgatt
     3481 cagcaagtag gcaggattct tcggatggcc gatcgcctgc tggtcgatca ggtaggtttc
     3541 aaaggtaacg tgaccttggg gcttgagcgc tcttcggatc tgatgaatca aagagcgctg
     3601 cagataattg acattcacga tcaggttgta agcggcaggg ggaagttcaa ttcgttccag
     3661 gtccgcaagg tgccatctta tctcaagcga cttttccgct gcgcggcggc gcgcctcctc
     3721 aagagcgatc gttgaaatgt cgatgccaac cacgctgaag cccatttcgg ccaggaagat
     3781 tgcgttacgg ccctttcccg tcgcgacatc tagagccagc ccgcgaggga tgtgccaggc
     3841 atcactctcg agaacctgcc ttaagaaact cgacggctga tcggcgccag aggagaccct
     3901 atgttgacgg tcccacctcg ttttatcatc accagacatg attagatttg aagatgatcc
     3961 agtagttcca atcgttcaag ctgttcatgc cgtatgagtc gagcccgaag agaaccactc
     4021 tgagtagatc ctgattattt agacgattgg gcctattcaa cacgtccaag atcctctttt
     4081 gcttgattat gatgcactga tgcaggttgc tcaaaagact cggtggcgag atccgcgaaa
     4141 cgaaaataga tgagctgagc cgctacgtgt tagtacgttg aagcgagtag gttgaggaaa
     4201 tgaagcataa gagtcttttc agcaacctgc attaatacac tgcgcgcgca agggtcagca
     4261 caaagacctc ccgcgccccg ccccctttta gtacccggct gcactcgtta accgtagccc
     4321 ctgaagtgta cacgtcatcg actaacagca agcgtttatc ctccactgaa ctttctccag
     4381 cgagggcgaa tgcgccgcgc atgttttttc gtcggtcctc ttccggcagt tgcgtttgcg
     4441 gcggtgtgtc catttttcgg tagagcgtaa agcaatctaa gggaagattg taggcccgac
     4501 caatctggcg cgccagcaac acggattgat taaagccgcg ccagcgcagc cgccgtgggt
     4561 gcagcggcac aggaataatc gcttctgcct tacatgttgc aaggaactct tcacagccgc
     4621 gcgccattag ccgtcccaaa ggtttgccca gggcgacctt gcgtccatac ttaaattttt
     4681 gaatcacctg ccggagcgga tgctctgtcg cttcttctct gggatagcag gcccaggaat
     4741 gggctcgcgc gaagtgtgga tttcgcgcaa tgcaaactcc gcaggcatga tcatctccgc
     4801 tcgaatctgg aaacggccgg ccacaagtcg tacagaacgg atgcgccaca agctgaattt
     4861 gcggccagca ggtggaacaa aaatattcgt agtctcggcc ttggatcgaa ccgctgcacg
     4921 cccgacaacg cggtggataa agccaatcca ccatcaactc aaagcgctcg gcaaaagatc
     4981 ggtcccgtcc aggcaccatc actgcgccac tctacaaaat cagctcgtaa tcagacagag
     5041 cttcgctgag tcgaacagcg gccgaggtcg tgtcatcaat cggtaatcag cgagtgaccg
     5101 gtcatttcta acggttgggg caactcgaga aggtgcaaaa ctgtcggcgc cacatcgatc
     5161 gccgttcctt ccctgagacg gctttgtttg cgtttctcgt ccaccagtat gatcggaacc
     5221 cggttgatcg tgtgagcggt gtggggcatc ccagtatcgt aatcgattag ctgctcgatg
     5281 ttgccgtgat ctgcggtgat aaggactctc gccttccggc tcagcgccgc gtccaccact
     5341 ttaccgaggc actcgtcaat gacctcgcag gcttttactg aagcctcgaa attaccggtg
     5401 tgtccgacca tatcggcatt ggcgaaattc gcaatgacca ggttgatatg atcctcggag
     5461 aacttcttta ccagagcctc agtaacctca cgggcgctca tctcgggctt gaactcatac
     5521 gtcggaacat ctttcggcga gggaatcaga atgcgttgtt cgccaggaaa ttccttttct
     5581 tcgccgccat tgaagaagta cgtcacgtgg gcatattttt ctgtctcggc aatgcgtagt
     5641 tgcttgagcc cgtgctggct gacaacctcg ccaagaattt ttcgcaactc acgcggtgga
     5701 taggctaccg gcactttgaa cgtttcgtcg tactgagtca tggttgtgta ggtcgaaaga
     5761 ttcagacggt gcttgcgggg gaattccttg aaatcagcat cgataaacgc gcgggtgagc
     5821 tcgcgggcgc gatcagcccg gaaattgaag aatatgacgc cgtcaccatc ccttatcaat
     5881 ccttcagaaa ttgtgctccg gatcaccgcg ggaagaacga attcatcggt aacgccctca
     5941 ctgtaactat tacgaatagc ttccagcggt gaatcagaaa gcttgccggc gccttctgta
     6001 agacagagat aagccttttc gactcggtcc caccgtttat cccgatccat tgcgtaatag
     6061 cggcctgtga gcgttgcgat ctttacgtcc ggccaggttt ttagcttgtc attcagatcg
     6121 agcataaatt gctcggcgct attgggcggt gtgtcgcgcc cgtcaaggaa gagatgcaga
     6181 tataccggtg ccaccttttc gcgttgcgcc atctcgatta acgcttccat atggcgctgg
     6241 tggctgtgaa caccgccgtc ccccagaaga cccatcaggt ggagcctgcc gccttcggct
     6301 ttggtggttc tcaaagcgtt cgaaatgatc tgattcttgc caaagttgcc ctcatcgatg
     6361 tccttgtgaa tcagtgtaag gtcctgatag acaatccgcc cggcgccgag aatcatgtgg
     6421 cctacttcgg aattgcccat ttggccgtcg ggaagcccca catctaaacc ggacatcgac
     6481 aggctcgagg ttggataatt ccgtagcaac gagtccatgt ttggcatgga cgcattggcg
     6541 atcgcgttac cttccctttt tgggttgatg ccaaaaccgt ccaacacgac gagaataacg
     6601 ggttgattgg tcgccacagt agttcctttc atgtctcctg ccgttgatcg tcaatcgcgg
     6661 cgactatcag tcaaggcgag cctagaagag ttatggatgc tgctcagcgg cgatttg
//
LOCUS       DASUTG010000680         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1586633, whole genome shotgun sequence.
ACCESSION   DASUTG010000680 DASUTG010000000
VERSION     DASUTG010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586633"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..498
                     /locus_tag="VF089_15720"
     CDS             241..498
                     /locus_tag="VF089_15720"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEX6175456.1"
                     /translation="MKKPVPAEMMAQNTSDRWLQVAAYYIWEKEGYPHGRDLEHWYQA
                     SAQLADLSKGTKVATAKKSTSSRTAKKQIKSPVKKPPSLTT"
     gene            1345..2058
                     /locus_tag="VF089_15725"
     CDS             1345..2058
                     /locus_tag="VF089_15725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6175457.1"
                     /translation="MAETCVRQYSQDPGQLLCSVFAHEIANSLQVVSALAGSMERHLC
                     KIGRVDRPTGQSLRLIAEEIDRLTLLLNDFRSFQLFSLDLEPTSLANVIEDCLALKAS
                     EATQRSIGVDCNVPLDLPPIMADAAKLKQVFLNLYSNAFEAMRDGGILTVRTMEGEEK
                     VCLDIADNGEGIPAGMDIFEPFVTSKPHGTGLGLTVSRWIVLAHGGSISYTSKPGQGT
                     TFHLAFPISRSSSRLRPSG"
     gene            complement(2562..>4247)
                     /locus_tag="VF089_15730"
     CDS             complement(2562..>4247)
                     /locus_tag="VF089_15730"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX6175458.1"
                     /translation="GATDGHPAPELYAALAAAHGHGIVTLLVTGRVLEDVQRACEELS
                     PFDAVVAENGAIIHLCGIGRTIQLGQPPPGHFLGELRAQGVPFHTGAVIIGTWEQHAN
                     KLLELVRRFGLDGQLVFNRAALMLLPSGINKAMGIRRALHELGRSERSLIAFGDAEND
                     IPMLMNAEVGVAASGSVQSVSALADDFVSRPGGAGVSLYIGKIIEKHGIVATPKRRAV
                     VLGASADGSEVTLPSSGTNVVISGDPRSGKSWMAGLLSEQLIENGYRMCIVDPEGDYV
                     QMGQRPEVLTFGHDLALPSPGAVARLLSSSSLSMVLTMSSLAPDEQLSYVDQLFAVIR
                     EVRDTTGLPHWLVIDEAHYFFGAGSPGLNYLSSSTGNLCLVTYRPSLLADEVYESIGA
                     HIVTSTKVEEERYFMTKILQAHQHLGLQAHEALDALKSPSAGLLVMAAPKTPWRVFTP
                     VKRISLHAHHARKYADIRLPDDKAFNFLNAGEALAAHNILEFYEAVQNIPLSSLRHHL
                     LAADFSRWVGDIIGDQQLAQGLRKVERTTPAGATPDRAEILAHIRGHYQIQEQ"
BASE COUNT         1004 a         1161 c         1115 g          967 t
ORIGIN      
        1 ccgcctgcga acgggttgat taagcttgct acagagtgaa ttctcatagc atggaacata
       61 ccgctctcgt tttggcactg ctgcgaaaag gatgtgggtc ttccgagcga tccttatggc
      121 acgcgcattg cgcgtgcatc gagatgcgaa acgagtttag ttcagcttct gcggcggcag
      181 atggtgcggt tgggtaatgt agctcgacca ctaatggaaa ggaaagaaaa ggataaatcg
      241 atgaagaaac cagttcctgc cgagatgatg gctcagaata cttcagatag atggctgcaa
      301 gtggcggcat attacatttg ggagaaggaa ggatatccgc acggccgcga tctggagcat
      361 tggtatcagg ccagcgccca acttgcggat ctttctaaag ggacgaaggt cgccacggca
      421 aagaagtcga catcctcacg tactgcaaaa aagcaaatca agtcgcccgt aaaaaagccg
      481 ccctctctta ccacctagcc ccggattgtt cctcgtgaca gtcagcgaaa ggcgagccct
      541 ccgcttttga gcagcctgta ggcttctttt tcagcaagct gtcgttcctc tgctttgttc
      601 gttgcgccag gctctgcgcg agatgccccc ctctgctatc gagccgagga tctctgccgg
      661 ggaaatgtga tctcggattc gggaagttcg acgcacaacc aacaatctaa gtatgcccgc
      721 tgatttcaaa cttaaaaatt tacattcgat cacattccac acgaggggcg cagaagatta
      781 ttcggagcgg ggcgttgtgg aacacgcttg ttttcgttgt ccggtgcgac accctgttac
      841 aagcgatccg gctcgctgcg cctgagcttt atcgttcctt cgagccaatc gaacacgcca
      901 tcggcacggc cgatgagcag cgcgtgatcg aagaagttta tcagaagctg cctgtactaa
      961 atttctccaa aaatattctt gaggtgctta cgctgcagta tcgccaagcg cttgtagtcc
     1021 ttccggtacc cggagtcacc tggagcgatt ggggaacaag taatcgcata tcaaaattct
     1081 gcgccaattg ggcgcacccg actttgaccc cggacccgca atttcggagg aaagaaccat
     1141 acgtgtttcc taagcaagaa acctgggcgg cgatcaggaa tgacactcct gcaaaaagga
     1201 ttgacgttgc agcattctca tggcatgcgc attgcgttct taacgattgc gatccgaaac
     1261 gagttttcgt tcatattagt gctgttgtag tcgactcgct gattgcacgc aaagcgggcg
     1321 ctctgcattt aaaagggcag atcaatggca gaaacttgtg tccgacagta ctcacaagat
     1381 ccaggtcagc tactgtgctc ggtgttcgcg cacgagatcg ccaattcttt acaggtggtc
     1441 tccgctcttg ccggctcaat ggagcgacat ctctgtaaaa tcggccgcgt cgataggccg
     1501 acgggccaat cgctccgact cattgctgaa gagatcgacc gattaactct gcttctcaac
     1561 gattttcgct catttcaact tttcagcctg gatctcgagc ccacatctct ggcaaacgtg
     1621 atcgaagact gcttggcatt aaaagctagc gaagctaccc aacgcagcat tggtgttgac
     1681 tgcaacgttc ctttagactt gcctcctatc atggcggacg cggcgaaact taagcaagtt
     1741 tttttgaatt tatacagtaa cgcattcgaa gcgatgcgcg atggcgggat ccttactgtg
     1801 agaacaatgg aaggtgaaga aaaggtttgc ctcgacatag ccgacaatgg tgagggtatt
     1861 ccggcaggca tggatatttt cgagccattc gtgaccagta agcctcacgg tacaggttta
     1921 ggcctcacgg tttccagatg gatcgtcttg gctcacggcg gctccataag ctacaccagc
     1981 aagccaggac agggaactac ttttcacctt gcctttccaa tcagccgtag cagcagtaga
     2041 ctgcgcccct cgggctgaaa agtattcacc agaattgagg cggtcgaccg gcggtttggg
     2101 cggtagaatc ttcatgtcgt attcctctgg ggggcaactg ggccagagcg ccgatttact
     2161 tcggcagcgg ctatattgcg aagcgccagt tctggctgct cggaacttta ttcggtacgt
     2221 tttatctcgc ggctcttctc caatcggctt gccctatctc ctccttttcc gttcgtaatg
     2281 aagcagtaag cttcgacttc tgcaaagcgg tttactcata cgcgccggca cattggtacg
     2341 cctttcaaga aataggggaa aatggcgctt acaagggaac gctttgggcc agatcgagca
     2401 taagtttgct gcagatactg agatcgcagc cgtcgcaaac cgctgaggcc ggccgctcca
     2461 ccggccggcg gggacacgtc tttgaactga agttccgcta gtcactaact cgcggcaaca
     2521 tgcgcgggag cacgctgaac actgagcgtt cgactccgct ttcattgctc ttgaatctga
     2581 taatgacctc tgatgtgcgc cagaatctcc gcgcgatcgg gagttgcgcc cgccggcgtg
     2641 gtgcgttcca ctttccttag cccctgtgct agctgttggt cgccaatgat atcgcccacc
     2701 cagcgggaaa agtcagctgc aagcaaatga tgccgaaggg acgagagggg tatattttgc
     2761 actgcctcgt agaactccaa gatgttatgc gccgcaagcg cctctcctgc attgagaaaa
     2821 ttaaaagctt tgtcatcggg cagacgaata tccgcgtact tgcgagcgtg atgcgcatgg
     2881 agcgaaatac gtttgacggg agtaaaaact ctccaaggtg ttttaggtgc agccattacc
     2941 agcagtcctg cgctcggtga tttcaacgcg tcaagggctt cgtgcgcctg caaaccgaga
     3001 tgctgatgcg cttgaagaat ttttgtcatg aagtaacgct cttcctcgac tttcgtgctg
     3061 gtgactatat gcgcaccgat cgactcatag acttcgtcag cgagcaagct cggacggtag
     3121 gtcaccaagc acaagttccc ggtggagcta ctgagataat tcaacccggg gctacctgcc
     3181 ccaaagaagt aatgggcctc gtcgatcacc agccaatgag gcaacccagt agtgtctcgc
     3241 acttcccgaa tcaccgcgaa gagttggtcg acataactca gctgctcgtc aggcgctaat
     3301 gatgacattg tgagtaccat gcttagcgaa ctactgctca gtagcctcgc cactgccccc
     3361 ggggaaggta gagccagatc atgcccaaag gtcagaacct ctggacgttg tcccatctgc
     3421 acatagtcgc cttcaggatc gacgatgcac atccgataac cgttctcgat cagctgctct
     3481 gaaagcagtc cagccatcca agacttgcct gatcgcggat cgccgctgat gacgacattg
     3541 gtacctgaac ttggcaacgt cacctcagag ccatccgcag aagctccaag cacgaccgcc
     3601 cggcgttttg gtgtcgcaac gataccgtgc ttctctatga tcttaccgat atagagagaa
     3661 acgccggcgc cgccagggcg agaaacgaaa tcgtccgcca aggctgatac cgattgcact
     3721 gatcccgatg cggcaacgcc gacctccgcg ttcatcaaca tcggaatatc gttctcggca
     3781 tcgccgaagg cgatcaaact acgttcggaa cgccccaact catgcaatgc gcggcggata
     3841 cccattgcct tgttaatccc actgggtaaa agcatcagtg cggcgcggtt aaaaaccaac
     3901 tgaccgtcga gaccaaaacg acgaaccagt tctaacagtt tgttagcgtg ttgttcccaa
     3961 gtaccaatga taacggcgcc agtgtgaaac gggacgccct gagctcgcag ttcgccgaga
     4021 aaatgtcccg gcggcggctg accgagctga atcgtccgac cgatgccgca gagatggatg
     4081 atcgctccat tctcagccac tactgcatcg aatggcgata actcttcgca ggcgcgttgt
     4141 acatcttcca gaacccggcc ggttaccaga agcgtaacga tcccgtgtcc gtgcgccgct
     4201 gccaatgcgg cgtaaagctc cggcgccgga tgaccatcag tggctcc
//
LOCUS       DASUTG010000681         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1290222, whole genome shotgun sequence.
ACCESSION   DASUTG010000681 DASUTG010000000
VERSION     DASUTG010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290222"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF089_15735"
     CDS             <1..494
                     /locus_tag="VF089_15735"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175459.1"
                     /translation="IPYVPSTMPVVERMLELAEVRKDDFIYDLGSGDGRILILAAKKY
                     GARGVGIDLDPQRVAEARQKAKEEGVSHLIEFRAEDGTQTDISKATVVTLYMFKWFNN
                     AIKPKLQKLKPGSRIVAHDYDIDDWKPTAVVQLEPTKDPGIERHHTLYLWKVGSESAQ
                     RSD"
     gene            743..2083
                     /locus_tag="VF089_15740"
     CDS             743..2083
                     /locus_tag="VF089_15740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175460.1"
                     /translation="MPELTLSPPTTEEDVKSAGGGEVTHPTPTAPAGLWHKFSKTRAV
                     EALAHRDFRLLWLGHSFSSVAFWMDQVSRGWLIYELTDSTLQLGLVRGVQAIPTLFLS
                     PVAGSTADRYSRKMQIMIAQVADGLMLAALAVLIFTGRVEPWHVYVTAFGMAVVQAFH
                     QPARAAIIADTIPSSHLTNAIGLNSIIFNVARTTGPALAGVLIATFGTGGSYATQAVF
                     YLLATIWTIQLRPTQPSSLSAGGHAGLHGSFARSIIEGWRFSWQNEAVRTALLVVMFA
                     SLFIVPFTTLLPVFARDILGVGANGQGLLLTAMGVGALGTAVLIASVGDRMPRGLFML
                     GGVALYGISVVAFAGSPWFQLSMLLMVVVGFANVCSHALVQTVIQTYSPSEFRGRTMS
                     VFHMSNVVMTVGSMLIGALATLAGTRAAVALMGTAGALAMLAIHLALPRARHIR"
BASE COUNT          475 a          652 c          682 g          597 t
ORIGIN      
        1 cgattcccta tgtaccgagc acgatgccgg tagtcgagcg gatgctcgag cttgccgagg
       61 tgcggaagga cgattttatt tacgatctgg gcagcggcga cggccgcatt ctaattctgg
      121 cggcaaaaaa gtacggcgcg agaggcgtcg gcattgatct ggacccgcaa cgggtggctg
      181 aggcgaggca aaaggccaag gaagaaggcg tgagccatct gatcgagttt cgtgccgagg
      241 atggcactca gaccgacatt tccaaggcca cggtggtgac attgtacatg tttaaatggt
      301 tcaataatgc aatcaaacct aagctgcaga aattaaaacc gggatcgcgc atcgtcgccc
      361 atgactacga catcgatgat tggaagccga cagccgtggt acagttggaa ccgaccaaag
      421 accctggtat cgaaagacat cacacgctct atctgtggaa agtcgggagc gaatcggcgc
      481 agcgatccga ttagaaagcc cgttcaaaag ttcaacagtt caaggttcaa tgttcggcac
      541 ccacgggacg accaactacg ctttcgatct gcaaagtatt ctatcctctc atcgcgccga
      601 gtaattggtt gcgccggttg gcagaagctc aacagttcga cgttgaaggt tcggagtttg
      661 aatctgctaa ttgtgttgct acacactctc attgccggct cattgaacca ttgaactttt
      721 gaaccgttga cttgaaaggt cgttgccgga gttaaccctg agtccaccga ctacggaaga
      781 agacgtcaag tccgcgggag gaggcgaggt tacccatccc accccgacgg ctccggcggg
      841 tctctggcat aagttctcga aaacccgcgc cgtcgaggcg ctcgctcatc gtgatttccg
      901 gctgctttgg ctcggccatt cctttagctc ggtcgctttc tggatggatc aggtatcgcg
      961 cgggtggctg atttatgagc tgaccgactc aacgttgcag ctcggcttgg tccgtggagt
     1021 tcaagcgatt ccgactctgt ttctttctcc ggtagccgga agcacagccg atcgttactc
     1081 gcgcaagatg caaatcatga tcgcccaagt ggccgatggt ttgatgcttg cggccttggc
     1141 tgtgctgatt ttcactggcc gggttgaacc gtggcatgtt tacgtgaccg cgttcggaat
     1201 ggccgtcgtt caggcgtttc accagccggc acgtgcagcg atcattgccg atacaattcc
     1261 ctccagccac ctgaccaatg ccataggcct gaattcgatc atcttcaatg tggcgcgcac
     1321 cactggccca gctttagccg gcgtgctgat tgcgactttc ggaaccggcg gctcgtatgc
     1381 cacccaagcg gtgttttatc tgctcgcgac gatctggacg attcagcttc gtccgacgca
     1441 gccgtcttcg ctaagcgctg gcggccatgc cggactgcac ggttctttcg ctcgcagtat
     1501 catcgaaggt tggagattca gctggcagaa tgaagctgtg cgcacggctt tgctcgtcgt
     1561 gatgtttgcg tctctgttca tcgtaccgtt tacaactctt ctgccggttt ttgcgcggga
     1621 tattctcggc gtcggcgcca acgggcaagg gctgcttctc accgccatgg gcgtcggcgc
     1681 gttgggaacg gcggtgctga tagcatctgt gggcgaccgc atgcctagag ggctattcat
     1741 gctcggcggc gttgccttat atggaatcag cgtcgtagca tttgccggct ctccatggtt
     1801 ccagctttca atgctgctca tggtagttgt tggctttgcc aatgtttgtt cgcacgcgct
     1861 ggtgcaaacc gtgattcaaa cctactctcc gtctgaattt cgtggccgaa caatgtctgt
     1921 ctttcacatg agcaacgttg tgatgaccgt gggaagcatg ctgattggcg ccctggcgac
     1981 cctcgccgga actcgcgcgg ccgtcgcgct catgggaaca gccggggcct tggccatgct
     2041 cgcaattcat ctagcattgc cgcgcgcgcg gcatattcgg taagttccat tctatatttg
     2101 ctgctctaat ttccgtcttt gatggcggtc gatgaaacct tctctatcgc gactatgccc
     2161 ggagccgtgc taggagcgct tgccacaacc gcgccgcgaa cgactcaatt ttttctttgg
     2221 cttttgttgc ttgcaattgc tgccgtgttg gtgatcagtc ctggcaagaa agcgccaccc
     2281 gaattgcaca aagcagtcag cgatcgtcaa tccttcgcag cttggatatc gctactttag
     2341 tcatcggcgc gctggtgagc acggcttttg gatttttatc gagattcttg ggaatcggcg
     2401 gcggga
//
LOCUS       DASUTG010000682         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_402100, whole genome shotgun sequence.
ACCESSION   DASUTG010000682 DASUTG010000000
VERSION     DASUTG010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402100"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF089_15745"
     CDS             <1..379
                     /locus_tag="VF089_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175461.1"
                     /translation="DASFGGIAFLTITSFLSANAILSTHSLNETEIDWCSSNNSVPCA
                     VRSISVPILVVAMQGHYFIRDGEYIYETAASDDKEFIVLEGANHGLGPCAPCAALHNA
                     DYSNARINLFNHIRDWVNERYGV"
     gene            971..1921
                     /locus_tag="VF089_15750"
     CDS             971..1921
                     /locus_tag="VF089_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175462.1"
                     /translation="MMAFYQNIAENGAATCQRPERLIPCDTTNLSNLPKADGVILLDS
                     HLGDAVATMTYIDPAVVNETQPAVRNPRLDMFDPRNGFDPNTGVAQYGDDFIEAFLAR
                     QSERNNLLIDQALNQLRHIENGTGNLYPDDMPFVVPGSVGARLFQPDTRLLRCTKDNY
                     LLLTNANPAGEGPKQICSVRLSGGRAADALSFNSVTNTSVRAWLGAHSVLTTDEYNQT
                     PDDITGIVYESSNTSTPTNVEGITKPLLIVVMGAHYFMRPDEIILAHATNTNDKTMVA
                     VEGATHGFTPCGNCPGAPFGDTVKRLFDYVDTWLTEPERF"
     gene            2148..>2778
                     /locus_tag="VF089_15755"
     CDS             2148..>2778
                     /locus_tag="VF089_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6175463.1"
                     /translation="MTTAEKVIKTKVGLLELGKQLGNVSKACRVMGYSRDSFYRFKEL
                     YETGGEAALQEISRSKPNLRNRIAEKVESAVVELAIAEPAWGQVRVANELKKKAISIS
                     PAGVRCVWLRHDLENIKKRLKALEAKSLQEALVLTEAQIAVLEKAKEDKEAHGEFDSE
                     CPGYCGAQDTFYVGTLKGVGRIYQQTFIDTYSKVSIAKLYDRKTPIAAAD"
BASE COUNT          709 a          681 c          758 g          630 t
ORIGIN      
        1 tgacgcgagc tttggcggaa tcgcattttt gactataact tccttcctta gcgccaatgc
       61 aattctcagc acgcactcat tgaatgaaac tgaaatagat tggtgcagca gcaacaattc
      121 cgttccatgc gccgttcgaa gcatttcggt acctatcctg gtcgttgcaa tgcagggtca
      181 ctacttcatt cgcgatggcg agtacatcta tgaaaccgcg gcgagtgatg acaaagaatt
      241 catcgtactc gagggagcga atcacgggct gggaccttgc gctccttgtg cggcactaca
      301 caatgcggat tacagtaatg cccggatcaa tcttttcaat cacataagag actgggtaaa
      361 tgagcgttac ggagtgtaac gtggcgagtt gcgttgagca gacgccaacc gtcttttatt
      421 cttggtatgc atttgcctgc tcgtgaggaa aaccgaagct cgaagctgtg cgtcgaacca
      481 tgtcggcaat agccgaaggc cagtctgaaa ggaggacgcc atgatgtccc tactgttatc
      541 gcaattacgc ctaagcgcgg tcgccgcggc actgtttggc ttctcgtgca tatcctttgc
      601 gcaagccgca gttaccacca cgtatgtcaa tctgggtgcc ggcggtaatg ctttattgcg
      661 agtgccgtca cctctcgccg gcagcccgag agaccagatt ggtatctttg taatgcatcc
      721 gagctcgagc ttcatcaata gccccatatg cacagaactc gctaatcgcg gttatcgaac
      781 tctttgcgcc gacaatccgt tcagttttaa tgcgaatgcg tataagggtt atgaagatca
      841 ctcggcgata atcgcccgag ggataaactg cctcaaagcg atagcccctc caggacaagc
      901 gaactcgcct tgcacctcaa tttccggcat cacgaaaatc attctgatcg gtcacagcat
      961 ggccggtcca atgatggcgt tctaccaaaa cattgccgaa aacggcgcgg cgacttgcca
     1021 aaggccggaa cggctgattc catgtgatac cacgaacctc tccaatctcc cgaaagccga
     1081 cggtgttatt ctcctggatt ctcatttggg agacgcagtt gccaccatga cttatataga
     1141 ccctgccgtg gtaaatgaaa cgcaaccggc ggttcgcaac cctcgcctgg atatgttcga
     1201 ccctcgaaac ggattcgatc caaataccgg cgtagctcaa tacggagatg atttcataga
     1261 agcgttccta gcgcgacaat cagagcgcaa caatctgctt atcgatcaag cgctaaatca
     1321 gctgaggcac atcgagaatg gtacaggcaa tctgtacccg gacgatatgc cattcgttgt
     1381 gcccgggtcc gttggcgctc ggctgtttca gcccgataca aggctcctaa gatgcactaa
     1441 agacaactac cttctattga ctaatgcgaa ccctgcggga gaagggccaa agcaaatctg
     1501 ttctgttcgg ctttccggcg gaagagccgc ggatgcgctt tcttttaatt cggttacgaa
     1561 tacttcagtt cgggcctggc ttggtgcgca ctcagtcctc actactgatg aatacaacca
     1621 aacgcctgac gatatcacag gtattgtgta cgagtcttct aacaccagta cgccgaccaa
     1681 tgtcgaaggc ataactaagc cgttgctcat tgttgtaatg ggtgctcact acttcatgag
     1741 accagacgag attattcttg cccatgcaac gaacactaat gataagacga tggtggcggt
     1801 ggaaggcgcc acgcatgggt tcaccccctg cggtaactgt ccgggagcgc cgttcgggga
     1861 caccgtgaag aggctgtttg attacgttga tacgtggctt actgaaccgg aacgcttttg
     1921 agaatttacg caaactggac aggaggaaga accgtcgctc cgatcgcatc ggagcgacgg
     1981 tttgagggtg ctgtgaagat ttatggggca acttaagggg gcaacatgcc gctgtgggcg
     2041 ccagatgcgc ttcggtatag gtgtaagtgt taagatttag tcttacaggc atggtaaagt
     2101 cctcgcgatc ctcagactct gaaggagaga gaaaggaagc gaggaagatg acaacggcag
     2161 aaaaggtgat caagacgaag gtaggtttat tggagctggg caagcagctc ggcaatgtgt
     2221 cgaaagcctg tcgagtgatg ggctacagcc gcgacagttt ttatcggttc aaggagctct
     2281 acgagacggg cggagaagca gcgctgcaag aaatctcgcg gagcaagccg aacctaagga
     2341 atcggatcgc cgaaaaggtt gaaagtgcgg tagtggagtt agcgatcgca gagccggcgt
     2401 gggggcaagt gcgagtcgct aatgaactta agaaaaaagc catatcgatc tcaccggccg
     2461 gagtgcgttg cgtttggctg cgccatgatc tggagaatat caaaaagcgg ctcaaagctt
     2521 tggaggccaa gagcctccag gaggcgttag tcctgactga agcgcagatt gcggtgttag
     2581 agaaagccaa agaggacaaa gaggcgcatg gggaatttga cagtgagtgt cctggatact
     2641 gtggggcaca ggatactttc tatgtaggaa ccttaaaggg agtggggcgg atctatcaac
     2701 agaccttcat cgatacctat agtaaggtct ccatagccaa gctctatgat cgcaagactc
     2761 caattgcagc tgcggatc
//
LOCUS       DASUTG010000683         5301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1142267, whole genome shotgun sequence.
ACCESSION   DASUTG010000683 DASUTG010000000
VERSION     DASUTG010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142267"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(585..854)
                     /locus_tag="VF089_15760"
     CDS             complement(585..854)
                     /locus_tag="VF089_15760"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX6175464.1"
                     /translation="MSGRRLFALVGVSFLAVYREAFETVLFYQALWLQSQGTQALVIW
                     GFIGGTILVAALVWVIFKLGMKIPLRLFFGGSSALLYVSAFVFTG"
     gene            complement(1164..1403)
                     /locus_tag="VF089_15765"
     CDS             complement(1164..1403)
                     /locus_tag="VF089_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175465.1"
                     /translation="MRTLDGFELAEPALFAKNAAMGRDLEALFTEFRGLIPSGADVGR
                     VRDHYEKLDAGLLQAMEVLGGQPGKAAPSPSSMRH"
     gene            complement(1381..2178)
                     /locus_tag="VF089_15770"
     CDS             complement(1381..2178)
                     /locus_tag="VF089_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6175466.1"
                     /translation="MSEFAARSLELFSHLEAKENGDKANIENDLRTLATHIKNMSGEE
                     VVPQLAQQIKDRLIKTYSIITFPKAIPAYDGAKAIYQENCAQCHGTTGRGDGPAAASM
                     QPKEPTPVNLTDPELIKNLSPFKAFNTTTFGIQGTGMPSFSALSEEQRWEAAFYIFSL
                     RFSAEDAARGRELFAARKLSPELINPATPATSSDGELTAKLTAAMGSEQEALTVLAYL
                     RYGLLQDRKADPLLTTRTFLREAVELYQSGDQKAAYQRSVDAYPRWL"
     gene            complement(2409..4100)
                     /locus_tag="VF089_15775"
     CDS             complement(2409..4100)
                     /locus_tag="VF089_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6175467.1"
                     /translation="MTGLAALRSRIYVSYSPNRLGWLGWVSITTAALVIAPIVAVVTN
                     VYRPSATTWYHLVSTVLPEYIVNTFLLAFLVGIGVVFFGVISAWLVTAYYFPGHRLLE
                     WALVLPLAMPAYVMAYAYTDWLQAAGPVQTAIRDITGWQVREYWFPEIRSLPGAAAML
                     SFALYPYVYLLARNAFLDQSRTTLEAARLAGYGAWGRFWHVALPLARTGIVAGTALAL
                     METLADFGTVSYFAVNTFTTGIYRAWLSLGDPVAAGQLASCLLVFVLAMLTLERRQRG
                     GARYAGKRIPMPPQRLHGWFAASAFGFCAAPVLFGFLVPAGILLRLAINEPEAQLDAR
                     VYSLVANTFTLSGVAAIAALSVAVLLAYAGRVVRSPLVHGANRAAVLGYALPGAVIAV
                     GILVPLGKLDNTIAGWAYEQFGIKTGLLLTGSIAALIYAYLVRFLAVAFQTVEAGLTR
                     VTSSMDDAARSLGLSPARTLLRVHLPIMYGSLLTAGLLVFVDVMKELPATFAMRPFNF
                     DTLAVEAYNLAKDERIAEAAVPSLLIVGIALVPLILLSRQITRSSLMRREGAGHV"
     gene            complement(4146..5198)
                     /locus_tag="VF089_15780"
     CDS             complement(4146..5198)
                     /locus_tag="VF089_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6175468.1"
                     /translation="MHNAIRNRRIFTLIALTATLVLAPWIGEALAQEKVLNLYTARHY
                     VTDEAFYTGYTKATGIKINRIEGGEDALFERIKSEGVNSPADVFLTVDVARLWRAEQA
                     GIFEPLKSELLNKRIPAHYRAADNNWFGFSARARVIAYDQKKIKPAQLARYEDLADAK
                     WKGEICTRPSSHPYNLSLISSMILHLGEEKATEWARGVANNLARPPRGGDTDQLQAVG
                     AGECSIALSNHYYYVRLMRSEKPADRTVVEKVKLVWPNQDDRGTHVNISGGGILRNAP
                     HREAGVKFLEYLASDAAQTLFAKGNNEWPTVPGVKLANPELESFGKFKTDTLPLANLG
                     KTQATAQRIADKVGWK"
BASE COUNT         1213 a         1474 c         1494 g         1120 t
ORIGIN      
        1 gtgtcgcata cgccaggctt tttcaatccc tggatcaacc gttctcccaa gctgaaaaca
       61 caagcacgag agaacgcgca attccttcgg ggctctggtt taaaacgtcg gcccgcaatt
      121 agttcgttta tgcaaataga aatagtttgt caatactaac aaaaaaactt agtaaacttt
      181 accgcgcact cctcgcaaaa gatccgacgt ccgacaatgc tctgctgact gggcgaactg
      241 ctcaggtcga gtgaaatgac ggcttgaagt ttacagcggc acgtttgaaa ttgactccgc
      301 ccgacaagcg ccagcttagc aagttaccgt attcgcaacg aacctgcttt ggactggtat
      361 cgctgcagcc atttcacgaa gattcgctct caagcattag ccattgtttc cgcagcttgt
      421 gtgctccgcc agagccaaaa gagcgccata accaatgcca tcagtaggat actttgggcg
      481 agcagcgtct cgagcgttgg atagatgcct aaggtagtta tctgcggtgc ccagggtagc
      541 ggtgtttcac ggatccagcc gcccgcctgc aaatctttta cgccttagcc ggtaaagacg
      601 aacgctgaaa cataaagcag cgcgctgctg ccgccgaaga acaaccggag cggaattttc
      661 atgccgagct tgaaaatcac ccagacgagc gctgccacca gaattgttcc gccgataaag
      721 ccccagataa ccaatgcttg ggtgccttgg ctttgcagcc agagagcttg atagaagagt
      781 acggtctcga aagcctcgcg gtagacagcg agaaacgaaa cgccgacgag tgcaaacagt
      841 cgccggccgc tcaacgcttc ctcgacccaa gcctggatga aaacgatgcc agcgcttcgc
      901 ttccgctttc gaaattagcc aatagcttac ggagaagagc acgagggcgg ccaatagcga
      961 ggtgaaaccc tcgaccagtt ctctttgcgc gccgctaaat gtgattaccg tttgcgccaa
     1021 aaaccaggta acaattcctg agagtaaggc gagtatccat ccccaatgca cgtaccggat
     1081 gggctgaatc gctccgatcg atttgaggac cgcgatgatc gcggcgataa tcagcgctgc
     1141 ctcgagcccc tcgcggatga tgattaatgc cgcattgaag aaggtgaagg agccgctttc
     1201 cccggttgac cgccgagaac ttccatcgcc tgcaacaaac cggcgtcgag cttctcgtag
     1261 tgatcgcgaa cgcggccgac gtctgccccg ctgggaatga ggccgcgaaa ttcggtgaac
     1321 agcgcctcga ggtctcgtcc catggcagcg ttcttggcga acaacgcggg ttccgccagt
     1381 tcaaagccat cgagggtacg catccaccga tcgttggtat gcggcctttt gatcgccgct
     1441 ctggtaaagt tctacggctt ctcgtaagaa ggttcgggtg gtaagcaggg gatcggcttt
     1501 gcggtcctgc aacaaaccat atctcaggta ggcaagtact gtgagcgcct cttgttcact
     1561 gcccattgca gcggttagct tggccgtcag ttctccatcg gaggaggtcg ccggagtagc
     1621 tggattgatg agttccggcg aaagcttgcg cgctgcgaag agctctctac cgcgggcggc
     1681 atcttcggct gaaaaacgaa gtgaaaagat gtaaaaggcg gcttcccatc tctgctcttc
     1741 gctgagagcg gaaaagctcg gcatcccggt cccctgaatc ccgaatgtcg tggtattgaa
     1801 agccttaaaa ggggagagat ttttgatcag ctctggatca gtgagattga cgggcgtggg
     1861 ctctttgggt tgcatgctgg cggccgcggg gccgtcgccg cgtccggttg tgccgtggca
     1921 ttgagcgcag ttctcttggt agatcgcctt ggcgccatca taggctggaa tcgctttagg
     1981 aaaagttatg atgctgtagg ttttgatcag gcgatctttg atctgctgcg ccagctgcgg
     2041 cacaacctct tcacccgaca tgttcttgat gtgcgtcgcc agcgtgcgca gatcgttttc
     2101 gatatttgcc ttgtcgccgt tctctttggc ctccaagtga ctgaatagct caaggctgcg
     2161 cgccgcgaac tccgacatct cctgatattc gtcacggtta atgacttgcc cgccctgcag
     2221 tgcgtttcga taatcaccgc agatataagt cgatcaggga taggagtctc ttcgcttggt
     2281 catcggccca gcaaaatgaa acacttgcgg caagcaggag aacagcgcca acgagagcgc
     2341 gcatgatgtt tctttggaaa tatctagctc ccggcattgc cgccgaggtt ctcgtggttt
     2401 tggtagtttc aaacatgacc ggcgccttcg cgccgcatca ggcttgagcg cgtgatctgg
     2461 cgcgagagca atataagcgg cacaagggcg ataccgacaa tcaacaatga cgggaccgct
     2521 gcttcggcaa tccgctcatc ttttgccagg ttgtaggcct ccaccgccag tgtgtcgaag
     2581 ttgaaagggc gcatcgcgaa ggttgcgggg agttctttca tgacgtcgac aaaaacaagc
     2641 aggccagcgg tcaaaaggct cccgtacata atcgggaggt gcacccgcag gagcgtgcgc
     2701 gccggtgaaa gaccgaggct gcgcgcagca tcgtccatgg aagatgttac tcgggtgagc
     2761 cccgcctcca cagtctgaaa tgccacagcc agaaaacgca ccagatacgc gtaaatgaga
     2821 gccgcaatcg agccggtgag caatagcccg gtctttatgc cgaactgctc ataggcccag
     2881 ccggcgatgg tgttgtctaa ctttcccagc ggcacgagaa tgcccactgc gatcaccgcg
     2941 ccgggcaagg catagcccag aaccgccgcc ctgtttgccc catggacgag gggactccgg
     3001 accacccttc cggcatacgc gagcaacacg gctactgaca gagccgcaat ggctgctacc
     3061 ccggaaagtg taaaggtatt tgcgacgagt gaataaaccc gcgcgtccaa ttgtgcttct
     3121 ggttcgttga tcgcaagcct gagcaggatg ccagcaggta ccaagaagcc aaataggacg
     3181 ggagccgcgc agaaaccgaa cgcgctagcc gcgaaccagc cgtgcaaccg ctgcggcggc
     3241 atcggtatgc gctttcctgc atagcgtgct ccaccacgct gcctccgttc caaggttagc
     3301 attgccaaca caaacaccag aagacaactc gccaactgcc cggctgcgac cggatcaccc
     3361 agtgagagcc aggcgcggta gatacccgtg gtgaatgtgt tcaccgcaaa ataagagacg
     3421 gtgccaaaat cagcgagggt ttccattagc gccagggcag tgccggcgac gatgccggtg
     3481 cgggcaagtg gcagcgctac gtgccagaat cggccccacg cgccataacc ggcaaggcgg
     3541 gcagcctcaa gcgtagtccg cgactgatca aggaaggcgt tacgcgcaag caagtagacg
     3601 taagggtaca gggcaaatga gagcatggcc gcggcgccag gaagcgagcg aatctccggg
     3661 aaccagtact cccgtacttg ccatccggtg atatctcgga tcgcggtctg caccggaccg
     3721 gcagcctgta gccaatcagt gtaggcgtac gccatgacgt acgcgggcat ggccaagggc
     3781 agaaccagcg cccactccag gagacgatgt ccggggaaat aataggcggt cacgagccaa
     3841 gcggaaatga cgccgaagaa gaccacgcct atacccacga gaaacgccag tagaaaggtg
     3901 ttgacgatgt attctggaag gaccgtcgag acgagatgat accaggtggt cgcactgggc
     3961 cggtagacgt tcgtaaccac cgccacgatc ggtgcgatca ccaaagcggc ggtggtgatg
     4021 gacacccaac caagccagcc gagccgattg ggagaatagc tgacgtatat ccgtgagcga
     4081 agtgcggcga ggccggtcat tactcgacta gctgcacgtg cgtgacaggc gcgctaagcc
     4141 tgtcgtcatt tccagcctac cttgtcggca atgcgctgtg cggttgcttg ggttttaccg
     4201 aggttcgcca gtggcagcgt gtctgttttg aacttgccga aggactcgag ctccgggttc
     4261 gccagcttca caccgggcac tgttggccat tcattgttgc ccttggcaaa taatgtctgc
     4321 gctgcatcgc tcgccaggta ctcaaggaat ttgacgcccg cctcgcggtg tggcgcgttt
     4381 cgcagaattc cgccgccgga aatgtttaca tgcgtgccgc ggtcgtcttg attgggccat
     4441 acaagtttaa ctttttctac gacggtacgg tctgcaggtt tttccgagcg catcaagcgg
     4501 acataataat aatggttcga gagagcgatc gagcactcac cggctccgac cgcctgcaat
     4561 tggtccgtat cgccgcccct aggcggacgc gcaagattat tggcgactcc acgagcccat
     4621 tcagtcgctt tctcctcacc gagatgcagt atcatgctcg aaatcagcga caaattgtac
     4681 ggatggctgc ttgggcgagt gcagatttcg cccttccatt tcgcgtcagc caaatcctca
     4741 tagcgcgcaa gctgagctgg cttgatcttt ttttggtcat aggcgatgac tcgagcgcgc
     4801 gccgaaaagc cgaaccagtt gttgtcagca gcgcgataat gggcaggaat ccgtttgtta
     4861 agcagctccg atttgagggg ctcgaagatt cctgcttgtt cggcacgcca caaccgcgcc
     4921 acatccacgg tcaagaaaac gtcggctgga ctgtttacgc cttcgctctt gatgcgctcg
     4981 aaaagcgcgt cctcgccgcc ttcgatacga ttgattttga tacccgtggc ttttgtgtag
     5041 ccggtataga aagcctcatc ggtaacgtag tgacgcgcgg tataaagatt gagcaccttc
     5101 tcttgggcca gcgcctctcc aatccatgga gccagcacca atgtggcagt caaagcaata
     5161 agtgtgaaaa ttctcctatt gcgtatcgcg ttgtgcattt gttcactcct tttcgaatca
     5221 tgaagttggc ccaaacttcg cacgatagtt tgtgctagca aactatttat cttcgattat
     5281 gcgaatgatc ggagtttgtc a
//
LOCUS       DASUTG010000684         4597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1586987, whole genome shotgun sequence.
ACCESSION   DASUTG010000684 DASUTG010000000
VERSION     DASUTG010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586987"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF089_15785"
     CDS             complement(<1..778)
                     /locus_tag="VF089_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX6175469.1"
                     /translation="MKSHDNALGSPAAESDSIILCSTSQGYSVRATPLRITRDVVVFE
                     VYNPYSILQLSEVLTAFKIIVNDQSIYSGRATVSNLVNTGIMLVCEASLEEGWSDIDL
                     LSLVSQPSKLRADFRSFLRQCERIHSIQPQYKVVVADMQNYFTELKRWLEHVELGIRS
                     LPSVDRSKTESSVINELAPSLLPSIDTLFGRFEETAADVPVQLLPSHRNYAKRQLHPH
                     LLCSPFAYRTYHKPLGYAGDYEMVNMILRDPNEGSTLFSKI"
     gene            complement(948..1023)
                     /locus_tag="VF089_15790"
     tRNA            complement(948..1023)
                     /locus_tag="VF089_15790"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Thr,seq:cgt)
     gene            complement(1024..1278)
                     /locus_tag="VF089_15795"
     CDS             complement(1024..1278)
                     /locus_tag="VF089_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175470.1"
                     /translation="MALAVALVAGWAASKSVASEWRSGVILWLFVGVAGLFLSQFVLL
                     FSGLQDYIEGVSEFRILFDLLAAYVASFFVAALIHFVRPL"
     gene            complement(1326..1955)
                     /gene="thiE"
                     /locus_tag="VF089_15800"
     CDS             complement(1326..1955)
                     /gene="thiE"
                     /locus_tag="VF089_15800"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX6175471.1"
                     /translation="MPRPDFNLYLITDRNQTKGRDLLWLIEEALDAGVEAIQLREKDL
                     AGRDLFFLAERASRLCQDYHSSLFVNDRLDIALAVNAAGVQLGNASVPIRVARQLLGT
                     QKMIGVSTHSLNEAFEAQQQGADFVLFGPVYFTPSKATYGAPQGLNKLKEIVEKISLP
                     VYAIGGINLENILDLRSLGVSRVALISAIMSSDAPKKSTTDLLRMLGSA"
     gene            complement(1965..2738)
                     /locus_tag="VF089_15805"
     CDS             complement(1965..2738)
                     /locus_tag="VF089_15805"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX6175472.1"
                     /translation="MQDAFELGEKVYQSRLIVGTGKYKNFQETKKAIEVSGAEIVTVA
                     IRRVNITDPHKENLLDYLSPKKYTILPNTAGCYTADDAVRTCRLAREAGVGKMIKLEV
                     IGDDRTLFPDVPATLEAAKVLVKEGFIVLPYITDDPITAQKLQDIGCAAVMPLAAPIG
                     SGLGIRNPYNIRIILEQATVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAQDPI
                     LMAEAMRLGVEAGRKAFLAGRIGKKLYATASSPLEGVLA"
     gene            complement(2744..2944)
                     /gene="thiS"
                     /locus_tag="VF089_15810"
     CDS             complement(2744..2944)
                     /gene="thiS"
                     /locus_tag="VF089_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX6175473.1"
                     /translation="MKIHINGEEKEIVDGLNIAALLDELEIRPGRVVVELNRSIISRD
                     AQRSTSLKEGDVLEIVHFVGGG"
     regulatory      complement(3033..3142)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3281..4177)
                     /locus_tag="VF089_15815"
     CDS             complement(3281..4177)
                     /locus_tag="VF089_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6175474.1"
                     /translation="MGPQDQIIDLAEVNRRFLKNGNASYLTSMQAFIEGGARALQVAR
                     QAEKYVAAKNIADQKKLAAVGALLRPNQAKIIAPIPVPKKNVVMLGINYREHVDEGAR
                     ARSLEIKYPDYPVFFTKPSTSIIGQFGKVINHKVTAKLDWEVELAVVIGRRGRDIPRE
                     EAYDYVFGYTVCLDMTARELQRQHGQWFKGKSLDTFCPMGPWIVHKSALKNPQQVQLI
                     CRVNGEVMQDGNTRDMIFDIPTTIESLSQGLTLEPGDMISTGTPSGVGFARVPPMFLK
                     PGDKVEGEVEGIGVLEVEIVAP"
     gene            complement(4405..>4597)
                     /locus_tag="VF089_15820"
     CDS             complement(4405..>4597)
                     /locus_tag="VF089_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175475.1"
                     /translation="TRSQQKSLSATVLYWRRGRFHAFTRVVSIGCRYNVGQMQIPKPC
                     QHVKLLNRNIAPSALEFQS"
BASE COUNT         1106 a         1222 c         1157 g         1112 t
ORIGIN      
        1 tgatcttaga aaatagggtg cttccctcat tggggtctct caggatcatg ttcaccatct
       61 cataatcacc cgcgtaacct aagggcttat ggtaggttcg gtaagcaaac ggggagcata
      121 gtagatgcgg atggagctgc cgcttagcat aatttctatg gctcggtaag agttgtaccg
      181 gcacgtcagc ggctgtttct tcaaaacggc cgaagagtgt gtcaatcgag gggagaaggc
      241 taggcgccaa ttcattgatc accgaagatt cggttttcga acggtcaacg gacggtaacg
      301 atcggatacc taattcaacg tgctcgagcc atcgcttgag ctccgtaaaa tagttctgca
      361 tatctgcgac aaccactttg tattgcggtt gaatgctgtg aatcctctcg cattgacgga
      421 gaaaactcct gaaatccgca cgcagtttac tcggctgaga taccaggctg agaagatcga
      481 tatctgacca gccttcctcg aggctggctt cacagacgag cataatacct gtgttaacga
      541 gattactcac cgttgcacga ccggaataaa tcgactgatc gttaacgatg attttaaacg
      601 ccgttaatac ctcggacagc tgaagaatgc tgtaagggtt gtagacttca aaaaccacta
      661 catcgcgtgt tattcgaaga ggggtagctc tgacgctgta gccttgactg gtggaacaga
      721 ggatgatgct gtcactctcg gctgcaggag acccgagggc attgtcgtga ctcttcatag
      781 acagagcagc actcgcatcg aagggctgat ttcttttatt gctccaccca ctcttcgtca
      841 atagtgccga gatcccggcc agagcgcatc tgcaccatag aaggtaggaa agccgcggaa
      901 agattcattg gcaacgtaag cgctgggatt cctcaggcga ctcggactgg agccacccgc
      961 cggagtcgaa ccggcgacct actgattacg aatcagttgc tctaccaact gagctagggt
     1021 ggcttagagg ggtcttacga aatgaatcaa agccgcgaca aaaaatgatg caacgtaagc
     1081 ggcgagtaga tcaaacaaga tccggaattc agagacaccc tcaatataat cctgcagacc
     1141 agaaaaaagc aacacaaact ggctcaggaa taaaccggcc acacctacga acagccacaa
     1201 aatgacccct gaacgccact cacttgcgac ggatttgctt gccgcccaac cggctactaa
     1261 tgctacggcc aaggccacga gcagcgatcc tccgagataa tccaggactt gctgcgcagt
     1321 ctctttcacg cgctcccaag cattcgtaag agatcggttg ttgatttttt tggagcatcg
     1381 gaactcatga ttgcagaaat gagcgccacc cgagaaacac ccaacgatct gagatcgaga
     1441 atattttcca agttaatccc gccgatagca taaaccggca gtgagatttt ctccactatt
     1501 tcttttagtt tgtttagccc ctgaggcgcc ccatacgtgg ctttagacgg ggtaaaataa
     1561 accggcccga ataatacgaa atcagcaccc tgctgttgcg cttcgaacgc ttcgttaaga
     1621 gaatgcgtcg acactccgat cattttttgc gttcccaaca gttgtcgggc gacccggatc
     1681 ggcactgagg catttcccaa ctgtactccg gcggcgttca cggccagcgc aatgtcgaga
     1741 cggtcattaa caaacaatga ggaatggtaa tcttgacaga gcctgctggc tctctcggcc
     1801 aagaagaaca gatcccgacc agccagatct ttttccctta actgaatcgc ttcgacgccg
     1861 gcatcgagag cttcctctat tagccagagt aaatcacgtc ctttggtttg gtttcgatcg
     1921 gtgatcaggt agaggttgaa atcgggcctc ggcacttggg tgcctcacgc caaaacgcct
     1981 tcgaggggac tggaagcggt ggcgtagagt tttttgccga tacggccagc aagaaaggct
     2041 ttgcggccgg cttcgacccc gaggcgcatc gcctcggcca tgaggatcgg atcctgggcc
     2101 ccggcaatgg ctgtgttcat caagacccca tggcagccca attccatggc aatcgccgcg
     2161 tccgaagcag ttcccacccc tgcgtcaaca ataaccggaa ccgtggcttg ttccagaatg
     2221 atacgaatgt tgtaaggatt gcggatccct aagcccgagc cgatgggcgc cgccagcggc
     2281 atgaccgcgg cacaccctat gtcttgcagt ttctgagcgg taatcggatc gtcggttata
     2341 taaggaagca cgatgaagcc ttccttaacc agtaccttgg cggcttccag agtagcaggc
     2401 acgtctggaa aaagggtcct atcatcgccg attacttcca attttatcat cttcccaact
     2461 cccgcttctc gcgctaatcg gcacgtacga acggcatcgt cggcggtgta acagccggca
     2521 gtgttcggga gaatcgtgta ttttttggga ctgagatagt cgagaagatt ctccttgtga
     2581 ggatccgtaa tgttcactcg ccgtattgca accgtgacga tctcagctcc agagacttcg
     2641 atggccttct tggtctcttg aaagttcttg tacttcccag ttccgacgat gagccgtgac
     2701 tgataaacct tttcgcccaa ctcaaatgca tcttgcatag cttttagcct ccacccacaa
     2761 agtggacaat ctcgagcacg tctccctctt taagcgacgt cgatctctgc gcgtcgcgcg
     2821 atatgatact gcgattaagc tctaccacga ctcgaccagg acgaatttct aactcgtcca
     2881 ggagtgccgc tatgttgagt ccatcgacga tttctttttc ttctccgttg atatggatct
     2941 tcataaaaaa accgtattca ctttgaccac cggaaggtgg ttgggaatac ggtctcaagt
     3001 ttgtcccccg ggcgtattag aaccagaacg ggaccatgac tttcacgctt ccctacgccg
     3061 gcgcaaaccg gatcaggttc gaagggttac ctgcgatggc aggctctcag tccgacttaa
     3121 gcagacaccc ctagcttggt gcacaattaa ctaacagagc ataaaaacct agtcaactcc
     3181 gttccggtaa tagaagcaag tagctgtcgg tcaggtaaac aactgttgaa tgtatggcaa
     3241 agcaaggtca actcataggc atatgggctc ggtgcgctaa ttagggcgca actatctcaa
     3301 cttccagaac tccgatccct tccacctccc cctcaacctt atccccgggc tttaagaaca
     3361 tcggcggaac ccgggcgaat ccgacgcccg aaggcgttcc ggtgctgatc atgtctccag
     3421 gctcgagggt caaaccctgc gatagtgatt ctatggtcgt gggtatatca aagatcatgt
     3481 ctcgtgtgtt gccgtcttgc ataacttcac cattgacgcg gcagataagc tgcacttgct
     3541 gcgggttttt gagggcgctc ttgtgaacga tccaaggtcc catggggcaa aaagtatcta
     3601 atgattttcc tttgaaccac tgaccatgct gacgttgtaa ttcacgggca gtcatgtcga
     3661 gacatacagt gtaaccgaaa acatagtcgt acgcttcctc tctaggaatg tcacggccgc
     3721 gcctgccgat aacgacagcc agctcgactt cccaatccag ctttgccgtg actttgtgat
     3781 tgatgacttt accgaactgt ccgatgatgc tagtcgatgg cttggtgaaa aaaaccgggt
     3841 aatctggata ttttatctca agggatcggg ctctggcacc ttcgtcaacg tgttcccggt
     3901 aattgatgcc gagcatgacg acgtttttct ttggcaccgg aatgggcgca ataatcttgg
     3961 cctggttcgg ccttagtaac gcccccacag cggcgagttt cttttgatcg gcgatgtttt
     4021 ttgcggctac gtatttctcg gcctgacgcg caacctgaag tgctctagca ccaccttcga
     4081 tgaaagcttg catactcgtc aggtaggacg cattgccgtt tttcaaaaag cggcgattaa
     4141 cctccgccag atcgatgatc tgatcttgcg gtcccatcaa tccaatccgt cgttcattcc
     4201 ttcgagtaaa cgttacgaag cgcatagttg ccccccaatg agtcgatctg gaactctttc
     4261 gctgtcagtg cccgagaagc ttacaagggt ttccgccgtt tcgttgtcgc aggattcgat
     4321 gttggtctct gcacggattc tgcaggtctg gatcggcaaa acgcgcggca agtcgctccg
     4381 attggggcgt gctcggagtc aaagtcatga ttgaaactcc agcgcgctcg gggcgatgtt
     4441 ccgattcaac aacttgacat gttggcaagg ctttgggatt tgcatttgac cgacgttgta
     4501 cctgcagccg atcgagacga cccgagtaaa ggcgtgaaat ctccccctgc gccagtacaa
     4561 gactgtggcg cttagcgatt tttgctgcga cctggtg
//
LOCUS       DASUTG010000685         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2182531, whole genome shotgun sequence.
ACCESSION   DASUTG010000685 DASUTG010000000
VERSION     DASUTG010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182531"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VF089_15825"
     CDS             complement(<1..905)
                     /locus_tag="VF089_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX6175476.1"
                     /translation="MREIEEVLSKVRATSEHQPIFLQAIQEVFESIEPAVSRHPQYIK
                     HGILERMVEPERTIIFRVPWVDDQGKIRVNRGFRIEMNSAIGPYKGGLRFHPSVNSGI
                     LKFLAFEQTFKNALTTLPMGGGKGGADFDPKGKSDLEVMRFCQSFMTELFRHIGPDTD
                     VPAGDIGVGGREIGYLFGQYKRIKNEFSGALTGKGPNWGGSLIRPEATGYGAVYFAQE
                     MLNGRGDSLRGKVCAVSGSGNVAQYTIEKLNHLGAKPVTLSDSDGFIYDRDGINPEKL
                     AWVIGLKEVRRGRIREYAERFKAEF"
     gene            complement(1239..1907)
                     /locus_tag="VF089_15830"
     CDS             complement(1239..1907)
                     /locus_tag="VF089_15830"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEX6175477.1"
                     /translation="MRLKGKKAMQRFCQWSVVRSFSMASMIGLALIGVNAQAQIGPTG
                     KTVTVNGTVSSVTGSDITLTTHSGPVVVKFSGDTTIRNEVPVKFSDITSGMYVGATAV
                     KQPDSTFRASRLHIFSEDQRGTGEGHRPLSSAPQSGSTMTNANVETVEDVAVQNVKGR
                     MLTLKYKDGEIKVLVPEDTPVVKRVIGDRRLLTAGSTVSASGPPAEDGSIVALQITVR
                     APVK"
     gene            complement(2024..2494)
                     /locus_tag="VF089_15835"
     CDS             complement(2024..2494)
                     /locus_tag="VF089_15835"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6175478.1"
                     /translation="MNEIIVPAVSAHYVTEGDRLAGLVYQPKWDQACHTRAPGLFLTT
                     RITANLNLLMAASNKRLYPRTQGKKPGGMKYPIVSRKAGILVAMALELPGSIIGGLFI
                     GYLLDNYFNTSPWIMMALTTLGFTAACIRLVRWVRYFSEKRSETESADREQSAE"
     gene            2837..3154
                     /locus_tag="VF089_15840"
     CDS             2837..3154
                     /locus_tag="VF089_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX6175479.1"
                     /translation="MGSKLYVGGLPYSVTEGRLQEVFSTHGTVESANVISDKFTGQSR
                     GFGFVEMSSGSEAQSAISALNGTQLDGRTLTVNEAKPMAKRDNFGDGGGRGDRNGGGR
                     NRW"
     gene            3299..3943
                     /locus_tag="VF089_15845"
     CDS             3299..3943
                     /locus_tag="VF089_15845"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175480.1"
                     /translation="MNPIKVSSFTRDYLRTQPFASPSSASRAMPIFGWNTLDQLLTRN
                     PPDVLVVARGKLLELPTPRTLGEALDLLTSGVGLVIRRAERLDPGVAGLAASVTRHIP
                     GEVHVQLIVTPAGTYGFGWHYDDEDVFIVHTEGSKDYFFRDNTVERERPAGTPPDFTR
                     FGNEASPIGTARLLAGDWLYIPARWWHAAKCIEDSLSISLGIFPDPFWLAKIKK"
BASE COUNT          929 a         1109 c         1099 g          996 t
ORIGIN      
        1 aagaactctg ccttgaaacg ttcggcgtac tcgcgtatgc ggccgcggcg cacttctttc
       61 aggccgatca cccaggcgag tttttccgga ttgatgccgt cgcggtcata gataaagcca
      121 tcggagtcgg agagcgtgac gggtttggcg ccgaggtggt tgagcttctc gatggtatac
      181 tgcgccacgt tgcccgagcc cgaaacagca caaactttgc ccctgagact gtcgccgcgt
      241 ccgttgagca tctcctgagc aaagtaaacc gcgccataac cagtggcctc cgggcgtatc
      301 aaagagccgc cccagttcgg ccccttcccg gtcagcgcgc ctgagaattc gttcttgatg
      361 cgcttgtatt ggccgaacag ataaccaatc tcgcggccgc ccacgcctat atctcccgcc
      421 ggaacatccg tatccgggcc gatgtgacga aacaactcgg tcatgaagga ttggcaaaat
      481 cgcatcactt ccaagtcgct cttacccttc ggatcgaaat ctgcgccgcc tttgccgccg
      541 cccatcggca aagtcgtcag tgcgttttta aatgtttgct cgaaagcgag aaattttagg
      601 atcccggaat tgacactcgg atgaaatctc aggccgcctt tgtacggccc tatggcgctg
      661 ttcatttcga tgcgaaagcc gcggttgact cttatcttgc cctggtcgtc cacccagggc
      721 acacggaaaa tgattgtccg ctccggctcg accatgcgtt ccaagatgcc atgcttgatg
      781 tactgcggat gccggctcac cgccggctcg atggattcaa agacttcctg gatcgcttgc
      841 aaaaaaatcg gttgatgttc actggtcgct cggactttgc tcaatacctc ttcgatctca
      901 cgcatcatcg aaaaactgcc tcccgctgag ttgaatctcc gccttacgat gagccgcatt
      961 gccactggcg gtaatttcta ggtaaccaaa aaccgcatag gattctactg cggcgcaaag
     1021 atgcggacag atgagatgca ttgattttct tgatggggaa ggaacgcggc gaggtgcaag
     1081 gggctacaat gggcactcga gaggaagagt gttagccgga agccgagatt tagaagagcg
     1141 cattccaaca gatcgtttga gttcaacact tacccaaggc ctcgaactgg agtattgaat
     1201 cagataatcg gatctaacac tccattggtt tttgcccgct acttaaccgg cgcgcgcacg
     1261 gtaatctgca gagctacgat cgagccgtcc tcggctggag gaccgctggc cgacactgtg
     1321 ctcccggctg taagcagcct tcgatcgccg atgacgcgct tgacgaccgg tgtgtcctcc
     1381 ggcacgagga ctttgatctc gccgtccttg tacttgagcg tcagcatccg gcctttaacg
     1441 ttttgtaccg ccacgtcttc gacagtttca acattggcgt tggtcatcgt cgagccgctc
     1501 tgcggcgccg agctcagcgg cctgtggcct tctccggtgc cgcgctggtc ctcggagaaa
     1561 atatgcaagc ggctcgcgcg gaaagtgctg tccggctgct ttaccgctgt ggctccgaca
     1621 tacatgccgg acgtgatatc ggaaaatttc accggcactt cattgcgaat agtagtgtcg
     1681 ccggaaaact tcacaacgac cggcccgcta tgggttgtca gcgttatgtc tgacccggtg
     1741 accgaggaga ctgtgccatt taccgtgact gttttgccgg tcgggccgat ttgagcttgc
     1801 gcgttcacgc cgatcagcgc cagaccgatc atcgaggcca tcgagaaact acgaacaaca
     1861 ctccattgac agaatctttg catcgctttt ttccctttca atctcatcgc ctttcgctcc
     1921 ccaagccggc ggcgtattgt gatcccgcgt atctgctgga cggtcgataa ttgactcatg
     1981 atcagagcac gacactgtcc cattctttta aaattcccgc gcatcattca gcagattgct
     2041 cgcggtccgc tgattcggtt tcgctacgct tctcggaaaa atacctgacc caccgcacta
     2101 ggcgtatgca tgctgccgta aagcctagcg ttgtcagtgc catcatgatc catggggaag
     2161 tattgaaata gttatcgagg agataaccga tgaacaatcc gccgatgata cttccgggaa
     2221 gctccagggc catagctact agaataccgg ctttgcgact aacgatggga tacttcatcc
     2281 caccaggttt ttttccctgg gtgcgcgggt atagcctttt gttcgaagca gccataagca
     2341 aattgagatt agcagttatc cgtgtcgtca ggaacaagcc aggcgcacgg gtatggcaag
     2401 cctgatccca tttaggttgg taaaccagcc cggcaagcct atcaccttcc gtgacataat
     2461 gggcacttac tgcaggaact attatctcat tcaaccagtg agatctattc cggttccatc
     2521 gaagcttaat ccgggtaagc gcaatatctg acgaactctg acgtgagaat ttatcagcaa
     2581 gcttatttca aaagtacaga gatggaggtt gacatcgtcc cccataacac gtacggtgaa
     2641 gcatcttctt cagtctacgg ccttctgggg aaagcacaaa tttgaaggcc tctttcagtc
     2701 tctatccttg cgaaactttc tttaaatgat attccgatca gggcgtggtt tagataattt
     2761 cttagtccat gttcttccca tttgcagtcg gattctcagg gtcaaagaca ataacagtct
     2821 cttataggag gagaacatgg gcagtaaatt atatgtaggt ggtttgcctt attcggttac
     2881 cgaagggcga ttacaggaag ttttctcgac gcacggcacg gtcgaatcgg caaacgtaat
     2941 ctcggataag tttaccggtc agtcacgcgg cttcggcttt gtcgaaatga gctctggaag
     3001 tgaggcccaa agtgccatct cggcgctaaa cgggacacaa ctcgatggcc gcactctgac
     3061 ggtcaacgag gccaagccga tggccaagcg cgataatttc ggtgacggcg gaggccgcgg
     3121 tgatcggaat ggcggtggac gtaatcgctg gtagtcctta gtcgaaacaa gtaacacgaa
     3181 agaggggagc aacagatcgg gttgctcccc tctttttttg ttcaacgtgt tgtcggattt
     3241 aaagtaggct ccccagccac gcttcaaact gttaatttga gtcatgctcg aagagtggtt
     3301 gaacccgatc aaggtcagtt cgtttacccg ggactacctg cgcacacaac catttgcgag
     3361 cccgtcatcg gctagtcgtg caatgccgat ttttggatgg aataccctcg accaactgtt
     3421 gacccgcaat ccgccggacg ttttggttgt ggctcgaggt aagctgctag agctaccgac
     3481 gcccaggact ttaggtgagg cactagacct tctgactagc ggagttgggt tggtgattcg
     3541 tcgtgcggaa agacttgatc ccggggttgc cggacttgca gcatctgtta cgcggcacat
     3601 tcccggtgaa gtgcatgttc aactgattgt tacacccgcc ggcacttatg gctttggctg
     3661 gcactatgac gacgaagatg ttttcatcgt acataccgaa ggctcaaagg actatttttt
     3721 tcgggacaat accgtggagc gtgaacggcc tgcaggcacc ccgccggatt tcactcgctt
     3781 cggcaatgaa gcctcaccga tcggcactgc tcggctgctg gcaggtgatt ggttatacat
     3841 tccagctcgc tggtggcatg cggcaaaatg tatcgaggat tcactttcga tttccctcgg
     3901 cattttcccc gacccattct ggcttgcaaa aatcaaaaag tgaaagcagc aaccatggag
     3961 agaggtcgat cccgcatggc acggttgctt tcaaacagca atagcagccg actgtgactg
     4021 ggttcggcgc ttacggaaac aggattgtta gtaggtgggg tcgcgccggc tgatcagaag
     4081 aagctgtgga cggccgatac ggggtggcgg aacagagaca tcatagccgc ggc
//
LOCUS       DASUTG010000686         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_254576, whole genome shotgun sequence.
ACCESSION   DASUTG010000686 DASUTG010000000
VERSION     DASUTG010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254576"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VF089_15850"
     CDS             <1..1210
                     /locus_tag="VF089_15850"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX6175481.1"
                     /translation="VDGRLYRLTDYRKIFVVGAGKGSARMCRALAELIGHWLYGGIII
                     TKYGYAIPVKNVAVMEAGHPIPDENGLRATEQVLELLRQTTREDLIINLISGGGSALL
                     CSPAEDVPFHEKQEITRLLLRCGAPIGEINAIRKHISKVKGGHLARLAHPSTLISLIL
                     SDVVGDSISDIASGPTAPDPSTFSECQSMLDRYKLRTEKSESIARLIDKGSAGEIEET
                     PKPGDPIFNNVVNVVIGSNRLALIAAKEQAETLGYYVKVIDDLAEGEATELAAAHAAV
                     IKKIYHSGKFRRPTCVISGGEATVTVRGNGLGGRNQEFAVATALQLDGLDGVVALIGG
                     TDGTDGPTEAAGGIVDGGTVGRGKTKGLDTRDYLSRNDSYHYLQATEDLLVTGPTFTN
                     VMDLRVFIID"
     gene            1419..1634
                     /locus_tag="VF089_15855"
     CDS             1419..1634
                     /locus_tag="VF089_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175482.1"
                     /translation="MIDVSLRGVRCRAAQEILDSDGTIGVATEGTIVYELEGEGGQLV
                     SVQWDTGTTCLVSSEEIEITDSSVSWQ"
     gene            complement(1708..4137)
                     /locus_tag="VF089_15860"
     CDS             complement(1708..4137)
                     /locus_tag="VF089_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX6175483.1"
                     /translation="MERYVCIHGHFYQPPRENAWLETVELQDSAYPYHDWNQRVTAEC
                     YAPNSVARILDEQGQIVQLVNNYTKISFNFGPTLLQWLENNEPEVYESILSADRLSMQ
                     RFAGHGSAIAQPYNHVILPLASRRDRRTQILWGIGDFERRFKRRPEGIWLPETAVDLE
                     TLEILAEEGVRFTILAPHQAARMRRAGARLWQDVTGAKIDPTRSYLQQLPSGKSIGLF
                     FYDGPIARAVAFEGLLTRGDHFADRLVGAFSDQRSWPQLVHIATDGESYGHHHRFGDM
                     ALAYALNQIESKKLAQLTNYGEYLARHPPTQEVEIIEKSSWSCFHGIDRWWSNCGCNT
                     GGHPSWNQEWRTPLRSALDWLRDSLSGCFETHAGKFLKDPWAARNDYVDVIQNRAPET
                     IERFFLRHAITPLDASSIATALKLLELQRHAMLMYTSCGWFFDELSGIETVQIVQYAA
                     RVIQLSQELFGESLESQFVEKLSVAKSNLSDRSGGREIYEQVVRTAMVNWERIGAHYA
                     VRSLFESYPDKTSIYCFRAERDDYRVFNAGTARLALGRVQLTSAITQESSMLSFGVLH
                     MGDHNVNGGVQEFMPDSVYQSLIAELLGPFTRVDFAEVIRVMDRCFGESNYSLRSLFR
                     EEQRSVINNILASSLGQSETLYRQIYEQRAPVMHYLTDLRIPLPRAFTAAAELVLNGD
                     LRRALQHQEINAERVTALLESAKIAGVTLDTSTLEFAYRQNLQRLANCLARRPAVSVL
                     RELRDATELLVKLPFAVDLWRVQNIFYVLVQSVYPQMQKRQLEGEQTAQEWVTVFEDI
                     AQRLSIKLPAS"
     gene            complement(4262..5509)
                     /gene="glgC"
                     /locus_tag="VF089_15865"
     CDS             complement(4262..5509)
                     /gene="glgC"
                     /locus_tag="VF089_15865"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX6175484.1"
                     /translation="MYVLGVVLAGGRGERLFPLTQDRAKPAVPFGGRYRIIDFVLSNF
                     VNSGILSIYVLTQFKAQSLLEHLDRGWRSTDFLGEHFVTAVPAQMRMGFEWYQGTADS
                     VYQNLYLIERHNPRVVAIFGADHIYRMNIRQMIDEHQRKNAATTVAALPVKINEASAF
                     GVIEVDPDWRIIGFDEKPSRPKSIPGEPDYALVSMGNYLFNTDVLVPALVADAKNNRS
                     VHDFGRNLLPDLIRDQKVFAYDFRRNRVPVPFKGEEASYWRDLGTIEAYYEANMDLCS
                     VDPSFNLYNRSWPLRTVGSGDPPAKFVFDWYDRKGMALNSIVSEGTIVSGSLVRNCVV
                     GRNVRIHSYSEVEDSVIMDWVEIGRNCKIRNAIIDKSNVIPSGTQIGYDVAHDQEHYF
                     VSDTGIVVVARGERKTSWIMTNP"
     gene            complement(5530..>6619)
                     /locus_tag="VF089_15870"
     CDS             complement(5530..>6619)
                     /locus_tag="VF089_15870"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX6175485.1"
                     /translation="TRSALEILRHEQVDLIHCHDWQTALAPVFLRTQPLRYPEIASAK
                     TVLTVHNIAFQGNFWRTDWHLLNLDEYLFSPAYLEFHQNFSLLKGGLIFADKITTVSP
                     THAAEIMTRDHGFGLEGVLQQRGNDLSGILNGVDYNQWNPAIDPLIAVQYSEDNLGGK
                     RHCKESLQEAMGLTIRPGVPLIGMISRLTSQKGIDLVGKVFAALIERDVQFVLLGSGE
                     SRYEDFFSRAAAQYPGKVGVRLGFDEPLAHRIEAGADIFLMPSLFEPCGLNQMFSLKY
                     GTIPVVRAVGGLKDTVEDYNVERSAGTGFVFEAYEPQELLKAIDRALELFPDKKSWIA
                     LQRRAMNMDFSWDRSARAYSRLYQQLLD"
BASE COUNT         1585 a         1763 c         1757 g         1514 t
ORIGIN      
        1 tgtcgacggt cggctttatc ggttgacgga ttatcgaaag atcttcgtcg tgggtgcggg
       61 aaaaggttcg gcacggatgt gtcgcgcact cgcagaattg atcggacatt ggctttatgg
      121 cgggatcatc ataactaaat atggctatgc gattccggta aaaaacgtcg cggttatgga
      181 agccggccac ccgattccgg acgagaatgg attgagagca acagaacagg tcctcgagtt
      241 gttgcggcag accacgagag aagatttaat aataaatctg atctccggtg gcggctcggc
      301 tttattatgc agccccgcgg aggatgtccc ttttcacgaa aagcaggaga tcacccggct
      361 cttgttacgc tgcggcgcgc ccatcggcga aattaatgcg atcagaaagc atatctcaaa
      421 agttaaaggc ggtcacctcg ccagacttgc tcacccttca acgctgatct cgttgatctt
      481 gtcggatgtt gtcggagatt cgatctcaga catcgcttcg ggccctacag cgccggatcc
      541 aagcacgttc tctgagtgcc agagtatgct tgataggtac aagctccgga cggaaaaatc
      601 cgagtccatc gcaagattga tcgacaaggg cagcgccgga gagatcgaag aaactccaaa
      661 gcccggtgat ccgatattca acaatgtcgt aaacgtggtg atcggcagta atcggctcgc
      721 gctcatcgcc gcgaaggagc aggccgaaac tctaggatac tatgtgaagg taattgatga
      781 tctagctgaa ggtgaagcga ctgaactcgc cgcggctcat gccgcagtga tcaagaaaat
      841 ttaccactcg gggaaatttc gccgaccgac ttgcgttatc tcgggaggtg aggcgaccgt
      901 taccgtccgc ggcaatggtt tgggcggtcg caatcaagaa tttgcggttg caactgctct
      961 gcaactcgac ggtttggatg gggtggttgc actaattgga ggaaccgacg gcacggatgg
     1021 gcccaccgag gcggcgggag ggatcgtcga tggcggcact gttggccgcg gcaagactaa
     1081 gggtctcgat acgcgagact acctcagcag aaacgactcc taccattatc ttcaggcaac
     1141 ggaggatctc ctagttaccg gaccgacctt tacaaacgtc atggatctcc gggtgtttat
     1201 aatagattaa ccagcgggac taagcgaaag tcatgtgatc gatctttgcg ggctgtttac
     1261 catggcgcat ttgggacttg ccgtgctgat ttccgacagt agacgagttc tgaaaccggc
     1321 ttgatttcga ttctgttcat gattagagtg cccgtctttc taggtaatcc ggtttcggat
     1381 caggacggtg ctctaaaaaa attgtcggga gttggaaaat gatcgatgtg agtttgcgcg
     1441 gagtcagatg ccgggcagcg caagagatcc tagatagcga tggcacgatc ggtgtcgcca
     1501 ccgaaggaac aatcgtctat gaactcgagg gtgaaggcgg acagctcgtc agtgtccaat
     1561 gggacaccgg aacgacttgc ctagtgtctt cggaggagat cgagataact gatagtagcg
     1621 ttagctggca gtgacgcggc tcggtaggag ccggacgggg atctaccgag ctgtcgcttc
     1681 aattgcagag tgagctatat caacagctca ggacgccggt agtttgatag acaacctttg
     1741 agcgatatct tcaaaaacag tcacccattc ttgtgctgtt tgctctcctt ccaactgacg
     1801 cttttgcatc tgcggataaa ccgattgcac caacacataa aaaatgttct gcactcgcca
     1861 tagatcgaca gcgaaaggta gtttgacgag taactcggtt gcgtcccgca attcgcgcag
     1921 gaccgagacg gccggtctgc gagctaggca attggccaac cgttgcaaat tttgccggta
     1981 ggcaaactcc aaagtagacg tatcgagcgt gactcctgcg attttcgcgg actccagtaa
     2041 tgcagtgaca cgctcggcat tgatctcctg atgttgaagc gcccggcgca gatctccatt
     2101 aaggaccagt tctgcggccg cagtaaaggc cctcggtagc ggaatacgca ggtctgtcag
     2161 gtagtgcata accggggccc gttgctcgta aatttgccgg tacaatgttt cgctctggcc
     2221 caaggacgaa gccaagatgt tattaattac gcttctctgt tcctcacgga aaagcgatcg
     2281 cagagaataa ttcgattcac caaagcagcg atccatgact cggattactt cggcgaaatc
     2341 gacgcgagtg aatggcccta gcagttcagc gatgagactt tgatagacac tatccggcat
     2401 gaactcctga actccaccat tgacgttatg gtcgcccatg tgcagcactc caaagctcag
     2461 catcgatgat tcctgagtga ttgcgctagt tagctgtaca cggcctagcg ccagcctggc
     2521 tgtgccggcg ttgaagactc gataatcgtc gcgttctgcc cgaaaacagt aaattgaagt
     2581 cttgtccgga tagctttcaa agagtgagcg caccgcgtaa tgggcgccga ttcgctccca
     2641 gttgaccatc gccgtgcgca cgacctgctc atagatctcc cttcctccgc tccgatccga
     2701 gagattgctc ttggcaacag ataatttctc gacaaattgc gactccaggg attcgccgaa
     2761 caactcctgg ctcaactgta tcacccgtgc ggcatactga acgatctgta cggtttctat
     2821 acccgatagc tcatcgaaga accaaccaca gctggtatac atcagcatgg catggcgctg
     2881 aagctcaagc agcttgagcg cggtcgctat ggagctggca tcaagcgggg taatcgcatg
     2941 acgtaaaaag aagcgttcga tagtttcggg tgcccggttt tgaatgacat ctacgtaatc
     3001 gttgcgcgct gcccagggat cctttaaaaa cttgccggcg tgagtctcga aacatcctga
     3061 cagcgaatcc cgcaaccaat ccagcgcact acgcaggggt gtccgccact cctgattcca
     3121 gctcggatgc ccgccggtat tgcacccgca gttgctccac caccgatcga tgccatgaaa
     3181 acaactccag gagctttttt cgatgatctc aacttcttgc gtcggcgggt gccgcgccag
     3241 atattcacca tagtttgtca gctgagccaa cttttttgat tcaatttggt tcagggcgta
     3301 agccagggcc atgtcaccga aacgatgatg atgcccatag ctctcaccgt cggttgcgat
     3361 atgcaccagt tggggccaac tccgttggtc cgaaaatgcg cccacgagcc tatccgcaaa
     3421 atgatcaccg cgcgtcagta aaccttcaaa agctacggcc cgtgcaatcg gtccgtcgta
     3481 aaaaaatagt ccaatgcttt taccggaagg tagttgttgt aagtaagagc gagtcggatc
     3541 aatcttggct cccgtcacat cttgccatag gcgcgcgccg gcgcgccgca ttcgcgccgc
     3601 ctggtgtggc gcaagaatcg taaagcgaac tccttcttcc gccaaaatct ctagtgtttc
     3661 caaatcaacc gccgtctccg gcaaccaaat cccttcgggt cgacgcttga agcggcgctc
     3721 aaagtcaccg atgccccaca ggatttgtgt ccgccggtcg cgccggctgg cgagcggcaa
     3781 tatgacatga ttgtaaggtt gagcgatggc tgagccatgc ccagcgaacc tctgcatact
     3841 cagacgatcc gcagataaga tcgactcgta gacttccggt tcattgttct ctagccactg
     3901 aagcaaggtc ggtccgaaat tgaaactgat tttggtgtag ttgttgacca gttgaacgat
     3961 ttgaccctgc tcgtcaagga tccgcgctac ggaattcggc gcataacatt cagcggtgac
     4021 gcgctggttc cagtcgtggt agggataagc ggaatcctgt aactcgactg tttccagcca
     4081 ggcattttcg cgcggcggct gataaaagtg tccgtgaatg caaacgtaac gttccatcta
     4141 gattgatccg gtttactgat gaatacgggg tgacatacgc gagaagatcg ggccaggtga
     4201 aaggagcgtc atgaaaagag aatctgggta aagagcgatg tttcggacga gttgcgtgcc
     4261 ctcaagggtt ggtcataatc cagctggtct tacgttcacc tcgagccacc acaacgattc
     4321 cggtgtcgga gacaaaataa tgctcctggt catgggcgac gtcgtagcca atttgcgtcc
     4381 ctgaggggat gacattggat ttgtcgatga tagcattgcg gatcttgcag ttgcggccaa
     4441 tttccaccca atccataatc accgaatcct cgacctcgct atagctgtga atcctgacat
     4501 ttcttccgac gacacaattt cgtaccaagc ttccactgac aatggttcct tcggagacga
     4561 tcgagttgag cgccattcct ttgcgatcgt accagtcaaa aacaaatttg gccggcggat
     4621 cgccggaccc caccgtgcgc aacggccagc tccgattgta aagattgaac gaagggtcga
     4681 ctgagcacag atccatattg gcttcgtaat acgcttcgat ggtgccgaga tcacgccaat
     4741 aactcgcttc ttctcccttg aacggcaccg gcacacgatt cctgcgaaaa tcataggcga
     4801 aaactttttg atctcgaatg agatccggaa gcagattgcg cccaaagtcg tgaacgctgc
     4861 ggttgttctt ggcatcggcg acgagagcgg gaaccagcac gtcggtattg aacaaataat
     4921 tccccatcga gaccaacgca taatccggct cgccgggaat agattttggc cggctcggct
     4981 tttcatcaaa gccgataatg cgccaatccg ggtccacctc gatgacacca aacgcgctcg
     5041 cctcgttgat tttcacgggt aacgcggcaa cggtagttgc ggcattcttg cgctgatgct
     5101 cgtcgatcat ctgacggatg ttcattcgat agatgtgatc ggcgccaaaa atcgcgacta
     5161 ccctcgggtt gtgccgttcg atcaggtaca gattctggta cacggagtcg gcagtgccct
     5221 gataccattc aaatcccatg cgcatctggg cgggcacggc ggtgacgaaa tgttcgccca
     5281 aaaaatccgt gctgcgccac cctcggtcga ggtgctccag cagggattgc gctttgaact
     5341 gggtcaaaac ataaattgat agtatgccgg agttaacgaa attggacaga acgaagtcga
     5401 tgatccgata tcttccgccg aacggcactg cgggcttagc ccgatcttga gttaacggaa
     5461 agagccgttc gcctctaccg ccggctaaca caactcctaa aacgtacatt gatggcctcg
     5521 tgtccctacc tagtccagca gctgctgata aagcctgcta tacgcgcgcg cggaacgatc
     5581 ccatgaaaaa tccatgttca tggctctgcg ctgtaacgca atccaggatt ttttatcggg
     5641 gaatagctcc aaagcacgat cgatcgcttt cagaagctcc tgcggctcgt aggcttcgaa
     5701 cacaaagccg gtaccggctg atcgctcgac attgtaatcc tccaccgtat cttttaaacc
     5761 gccgacggcg cgcaccaccg gaattgtacc atatttcaaa ctgaacatct gattcaatcc
     5821 gcagggttca aacagcgaag gcatcagaaa gatgtcagcg ccggcttcaa tccggtgcgc
     5881 cagcggttca tcgaaaccaa ggcgtactcc gaccttcccc gggtactgcg ccgctgcgcg
     5941 gctgaaaaaa tcctcatagc ggctttcacc gcttcccaac aatacaaatt ggacatctcg
     6001 ttcaatcagg gcggcaaaaa cttttccaac caggtcaatg cctttttgcg aggtcaaccg
     6061 cgagatcatg ccgatcaggg gcacgccggg tctgatcgtc agtcccatgg cttcctgcag
     6121 gctctctttg cagtgccgct taccaccgag attgtcttcg ctatactgaa cggcgatcag
     6181 cggatcgatc gcaggattcc attgattgta gtcaacgccg ttcaaaatgc cgctgagatc
     6241 gtttccccgc tgctgcagca cgccttcgag cccgaatcca tgatcccgcg tcatgatctc
     6301 ggcagcgtga gtcgggctga cggtggttat cttgtcggca aagatcaagc ctcccttcaa
     6361 tagactgaag ttctgatgga actccaaata ggccgggctg aaaagatact cgtccagatt
     6421 caggagatgc caatccgtgc gccagaagtt tccctggaag gcgatgttgt gcaccgttaa
     6481 aactgttttt gccgaggcga tctccgggta gcgcaaaggt tgtgtcctca agaataccgg
     6541 cgctagagcc gtctgccagt catggcaatg aatgagatcc acctgctcat ggcgcaaaat
     6601 ttccaatgcg ctacgggtg
//
LOCUS       DASUTG010000687         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2032303, whole genome shotgun sequence.
ACCESSION   DASUTG010000687 DASUTG010000000
VERSION     DASUTG010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032303"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VF089_15875"
     CDS             complement(<1..784)
                     /locus_tag="VF089_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6175486.1"
                     /translation="MELALEILPQVLLEGLVLGFMYALIALGYTMVYGVLEFINFAHS
                     EIFVLGAFVGVEVLFWLQDAGQLAVLHPAFVLSLVIACGMLISGLAAMALERVAYRPL
                     RASPRLVALISAIGASFFLQDALRLFESLWRNTFYLNYPTLDLLDQLLPVATNLVVPV
                     KSVMVIVTSLLSLGGLYYFVNRTRIGTAIRAVAQDPETASLMGIPVNRIIALTFFVGG
                     AMGGLAGVLFGLHYSLVNPYSGFAPGLKAFTAAVLGGIGNIPG"
     gene            968..2488
                     /locus_tag="VF089_15880"
     CDS             968..2488
                     /locus_tag="VF089_15880"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX6175487.1"
                     /translation="MKKALIAGATVFALLIATVIGLPTFVDLGRFKSSYLPLLEETLH
                     RRVDVGEMRLRLVPRPSIKLSDLKISGTRKLPAESFFTAQEVQLRLELWPLLRGRFEI
                     TELVLDKPVFTLQKASKRSSGDTDLADKKIPANTGAEPNKLGPTPKLPDSAALALTIP
                     RRLRIKDGQLEIVTEGRTPVHIHDIELSIEKFSRDRPFPYRASFNYPGLKTVTLGGQL
                     EYQEQQRKLILKDNRLTLQNLTLPLEGSISSLNTGPRFDLVLADDDVDARPVMQILEL
                     FGLAPTETEISGPMGLRISVSGPSNNLMTRIQGQFKNVKLHGERAIKGNLNGAVSLRL
                     ASGAGPELIKQVQGNGKLSAREGELTNVDLVKKIQRATGAIGFSAQQRREATTFKTLD
                     TEFILSDGVVEFKRIHLVNPQVELKGVGTMTLARQALNIGMDATLSPQASRKASRGRT
                     SQFFKNQRGQLVVPLTVTGNAQNPTVDLDSGKLAQRGATRSMERSLGTFFRQLFHR"
     gene            complement(2511..>3373)
                     /locus_tag="VF089_15885"
     CDS             complement(2511..>3373)
                     /locus_tag="VF089_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6175488.1"
                     /translation="SVLLVGPVLVFAALTITATLQSNSFVRNLLALEPFGTVMLMLLR
                     LVPYLILWGAFTLSYLFIPNTRVRLRSALVGGLVAAVLWQTIGWGFATFVTSSTRYYA
                     IYSSFAILLLFLLWLYVGWLIVLLGAQVSHAHQHIRFYYGQRENLARSPAGREKLALQ
                     LLLLIGRNFHQGLSPMSVTDLANRLQLPAALVKDLLEMFEQTRLVLPLADEETFVLGR
                     APEKIKIKEILDCIRSFGDVSKGTGLYLREPDDIDELLLALDQSVAATLEGRTLQSLI
                     VRQDPVTAVS"
BASE COUNT          886 a          944 c          900 g          643 t
ORIGIN      
        1 cgccgggaat attgccgatg ccgcccagga cggcggcggt aaaagctttc agccccggcg
       61 caaacccgct gtagggattg accaggctgt agtggaggcc aaataaaacc ccggctaaac
      121 ctcccatcgc gcctccgaca aaaaatgtca gcgcaatgat cctattgacc ggaatcccca
      181 tcagcgaggc ggtttccgga tcctgcgcca ccgcgcgaat cgccgtgccg atgcgcgtgc
      241 gattaacgaa gtaataaagg ccgcctaagc taagcagcga ggtaacaata accatcacgg
      301 acttcaccgg aacgacaaga ttagttgcca cgggcaaaag ctgatcgaga aggtccagag
      361 tcggataatt taggtaaaag gtgttgcgcc agaggctttc gaataagcgc agcgcatcct
      421 ggagaaaaaa tgacgcgccg atcgccgaga tcagcgcgac caggcgcggg ctggcccgga
      481 gcggacgata ggccacccgc tccagagcca tggcggctag tccgctgatc aacatgccgc
      541 aagcgatcac cagtgacaag acgaaggccg ggtgtaagac cgccagttgt ccggcatcct
      601 gcagccagaa gagcacttcg acaccgacaa acgccccgag aacgaagatt tcactatgag
      661 caaagttgat aaattccaag acaccgtaga ccatggtgta gccgagagcg atcaaagcat
      721 acatgaatcc caggacgaga ccctccaaca acacctgggg aagaatttcc agcgccaatt
      781 ccaacagcca gcggcccttt cgaagtttgt tagcctagca atactgccct tagtggctac
      841 ttttcgcaac gactttaccc taatgccgtc attgaatctg gcctgcggtc cgtataatgt
      901 ctcggcaagt gacgacaccg atgccaggca actgcgttaa ccggatcacc gagccggagg
      961 cgatttcgtg aagaaagctt tgatcgccgg cgcgactgtt tttgctctct taatcgcgac
     1021 ggtcatcggt ttaccgacgt ttgtcgatct cggccggttc aaaagcagtt atttgccctt
     1081 gctcgaagaa actctgcacc ggcgagttga tgtcggcgag atgcgcctac gcctagtgcc
     1141 gagaccttcg atcaaactat cggatctgaa gatctctggc acccgcaagc ttcccgccga
     1201 aagcttcttc actgcgcaag aggttcaact gcgcctcgag ttatggccgc tgttacgcgg
     1261 ccgcttcgaa ataaccgaat tggtgctgga caagccggtc tttacactgc aaaaggcatc
     1321 taagcgcagc tctggggata ctgatctggc ggacaaaaaa attccggcaa acaccggagc
     1381 cgaaccaaac aagctcggcc cgacacctaa actgccggat agcgccgctc tagctttgac
     1441 gattccgcgc cggcttcgga tcaaagacgg gcaattagag attgttactg aaggacgaac
     1501 ccccgtgcac atccatgata tcgaactttc aattgagaag ttctcacgcg atagaccctt
     1561 tccgtaccgc gcgagtttca attatcccgg cttaaagacg gttacattgg gcggacagct
     1621 cgaataccag gagcaacaaa ggaaactcat ccttaaagac aatcgcctga cgctgcagaa
     1681 cttaactctg cccctggagg gaagcatcag tagtcttaac accgggccgc gcttcgactt
     1741 ggttctggcc gacgatgacg ttgatgccag gccggtgatg cagattcttg aattgtttgg
     1801 tttagcgcca acagaaaccg agatttccgg gccaatgggc ttgcgcattt cggtgagcgg
     1861 cccgtcgaac aatctgatga cacggatcca aggccaattc aaaaatgtga aattgcatgg
     1921 cgaacgcgcg atcaagggga atctaaacgg cgcagtttcg cttcgtttag cctccggcgc
     1981 tggccccgag ctgataaagc aggtacaagg caatggcaaa ctatcggcgc gcgagggcga
     2041 attgaccaac gttgatctgg taaaaaaaat tcaacgcgcg accggtgcca tcggcttctc
     2101 cgcgcaacaa cggcgcgaag caaccacatt caagacactg gacacggaat tcattctgag
     2161 cgacggtgtc gtcgagttca aacgcatcca cctggtaaat ccgcaggtgg aacttaaggg
     2221 cgtcggaact atgaccctgg cgcgtcaggc gctcaacatc ggtatggacg caactctctc
     2281 ccctcaggct tccaggaagg ccagccgcgg cagaaccagc cagtttttca agaaccagcg
     2341 cggccaactg gtggtgccgc tcaccgttac cggtaatgcg cagaatccga cggtagatct
     2401 ggacagcggc aaattagcgc agcgcggcgc aacgaggtcg atggaaagga gtttgggaac
     2461 cttcttccgg cagctgtttc accgctgacg aacgcaagct aactggcaga ctacgagaca
     2521 gccgtgaccg gatcttgacg aacgatcaga ctctgtaggg tcctaccctc gagtgttgcg
     2581 gcaacagatt ggtccaatgc aagcagcagt tcatcgatat catccggctc tctcagataa
     2641 agaccagtgc ccttgctaac atctccaaag gatctgatac aatccaaaat ctctttaatc
     2701 ttgattttct cgggagcgcg gccgaggaca aatgtttctt catcagccaa tggcaagacc
     2761 agcctggtct gctcgaacat ctccaataag tccttaacca aagcggccgg caattggagc
     2821 cgattcgcca gatccgtgac actcatcgga ctgagtcctt gatggaaatt gcggccgatc
     2881 aggagcagca gctgcaacgc aagcttctca cgccctgctg ggcttcgcgc aaggttctca
     2941 cgctgaccat agtagaaacg aatgtgctga tgcgcgtgcg atacttgtgc tcctaataac
     3001 acaatcagcc agccgacata cagccagagg agaaacagca ggaggatggc aaagctggag
     3061 tagatagcgt agtaacgcgt cgatgaggtc acgaatgtgg caaatcccca accaatcgtc
     3121 tgccaaagta ctgcggccac gagaccgccg accagcgccg aacgtaacct aactcgggtg
     3181 ttcgggataa agaggtaaga aagcgtgaag gctccccaga ggatgagata gggaaccagg
     3241 cggagcaaca tcagcatgac tgttccgaat ggttccagcg caagcaaatt acggacgaaa
     3301 gaattgctct gcaacgtagc cgtaatcgtc agcgccgcga acaccagcac cgggccgact
     3361 agaaggacac tca
//
LOCUS       DASUTG010000688         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2032439, whole genome shotgun sequence.
ACCESSION   DASUTG010000688 DASUTG010000000
VERSION     DASUTG010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..981)
                     /locus_tag="VF089_15890"
     CDS             complement(22..981)
                     /locus_tag="VF089_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX6175489.1"
                     /translation="MRAMVLPRFGGSDLFEMHELERPEPDCGEVLVRVVASAVNPVDA
                     KLRANGGWAKLDPPVILGYDIAGIVEEIGPGVSDLRIGDEVYYTPEIFGNPHGSYAEY
                     NVASAAIVARKPTGLSFVEACAIPLAGGTAWEAVIRRLAVRPGETILIHGGAGGVGSF
                     AVQFAKAAGARVIASAGADNQDLLRQLGADVTIDYRKDDAAEITLRETYGYGADAAFD
                     IEGVDIVGRCLPAVRPFGRIACILPPQGDLSALYQKNITLHGTFLTRERHRLEEMRPL
                     FERKQAHPVIDMVLPLQEVGKAHDRLDSHHGRGKIVLQVESDR"
     gene            1179..2297
                     /gene="ald"
                     /locus_tag="VF089_15895"
     CDS             1179..2297
                     /gene="ald"
                     /locus_tag="VF089_15895"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011867841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX6175490.1"
                     /translation="MIIGVPKEIKTEENRVAVTPTGVSGFTARNHKVLVEKGAGVGSA
                     LSDRAYESAGAILIDSAKQVWSESDLIMKVKEPQVSEFPLLRPGLILFTYLHLAANEA
                     VTRALLDRKATAIAYETIQLEDGSLPLLTPMSEIAGRISIQVGAWCLQAENGGRGVLL
                     GGASGVRPGKVVILGAGIAGTAACQVAIGMGAHVSILDINPTKLRYVRDILGGQATTL
                     MSNRANVEEEVLDADLVVGSVLIPGAKAPKLISRLLVQRMKPGAAFVDIAIDQGGCAE
                     TSRPTTHRQPIYVEEEVVHYCVTNMPAIVPHTSTYALTNSTLSYGLELANRGFPQALA
                     RNRALAKGLNTFAGKVTHEGVAAAFNLPSLPLEEVIRA"
     gene            2294..3247
                     /locus_tag="VF089_15900"
     CDS             2294..3247
                     /locus_tag="VF089_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX6175491.1"
                     /translation="MRLVTRADFDGLVCGALLTKFESINAYLFVEPKFMQDGLVKIRA
                     GDIIANLPYHPACTLWFDHHVTNTTPDFAKPIVLGRGGFRLAPSAARVVYEYYKELSN
                     KPVGEEKTTRINNHEIIEFLDSERIKYLLHETDRIDAGKLEPQDVLDPHGYVLVSMTT
                     DGRNAGDEPYWLRIIELLRDASLEEVLRDPEVSRRCQRIRDEQEQLRKLLLARTTMKR
                     NVIYVDLRGIQEIPDGNRFLIFTLFPQGNIQVKVADDKQRENTTSISVGYNIFNTTSN
                     VNVGELLSHYGGGGHKVVGSCRVPNDQAETAIREIVAAVTD"
     gene            3347..3676
                     /locus_tag="VF089_15905"
     CDS             3347..3676
                     /locus_tag="VF089_15905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175492.1"
                     /translation="MKIVKIAAALKRLKKINDEIRYLSCFEQKRFTSGLIAFKPTKKA
                     NPKQISHDHQDVICQVLQGSGRLRIDGRRIPLAPGILCHIPKRTPHDFAAARSGELVL
                     LYLLIKT"
     gene            complement(3763..4068)
                     /locus_tag="VF089_15910"
     CDS             complement(3763..4068)
                     /locus_tag="VF089_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175493.1"
                     /translation="MPQFVVGQPIETETPVVEVTVTRDKPLPPGQHRFQLTVTDDAGN
                     ISTPAIVEVVVRDSQKPTAVLDAPRQVEAGQSFQLSGSRSTDIAPGRIVRYQWMMLS"
     gene            complement(4208..>4841)
                     /locus_tag="VF089_15915"
     CDS             complement(4208..>4841)
                     /locus_tag="VF089_15915"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEX6175494.1"
                     /translation="LAIGVESNVLLSRDSAKWRQLKELHRETYRAVKKMYPKLPVFFT
                     TEVLHYKRLARDAKQSEQEKEVAELMRHSDLFVMSLYPYMSLETPRPLPDHFLDFARR
                     FKKPIAVSESGMTSRDVPLKSYGLSLLGSEAEQRDFTELLLKTAARDKYEFVINFAST
                     DSDRLVNRLRPPLDDLARIWAFTGMQTSTGRPKPALAVWDGFLKAKYVRQ"
BASE COUNT         1110 a         1380 c         1288 g         1063 t
ORIGIN      
        1 gaaaaaaatt gcgattagga gttaacggtc gctttccacc tgcaagacaa tctttcctcg
       61 tccgtggtgt gaatcgagac ggtcatgcgc cttaccaacc tcctgcagcg gaagcaccat
      121 gtcgatcacg ggatgggctt gttttctttc aaacagaggc cgcatctcct ctaagcggtg
      181 gcgctcgcgc gtcagaaagg tgccatgcaa cgtgatgttc ttttgataga gagcagacag
      241 atcgccttgc ggcggcaaga tacaggcgat ccgaccgaac ggtcggacgg caggcaaaca
      301 gcggccgaca atatcaactc cttcgatgtc aaacgccgcg tccgcgccat agccgtacgt
      361 ttccctgagg gtaatctcgg ccgcatcatc tttgcgataa tcaatggtca catcggcgcc
      421 caattgtcgt aagagatctt gattgtcagc gcccgcgcta gcaatcaccc tcgcgcctgc
      481 ggctttggcg aattgaacgg caaacgaacc tactcctccg gcaccgccat gaatcagaat
      541 cgtttcgccg ggccgaaccg ctaacctgcg gattacagcc tcccacgccg tgcctccggc
      601 aagcggaatc gcacacgctt caacaaatga cagaccggtt ggtttgcgag cgacgattgc
      661 agcggatgcg acattatatt cggcgtaact cccgtgtggg ttgccaaaaa tttcaggggt
      721 gtagtagacc tcgtcgccta ttctaagatc gcttaccccg ggtccaatct cttcgacgat
      781 gccggcaatg tcgtacccta gaatcaccgg aggatccagt ttggcccagc caccattggc
      841 gcgcagcttt gcgtccactg gattaaccgc cgaagctaca acacgaacca gaacctcgcc
      901 gcagtccggt tcaggacgtt ctaattcgtg catctcaaag agatccgatc cgccgaaccg
      961 tggcagaacc attgcgcgca tatccgatcc tttcacccga gcacgttacg ttatcttatg
     1021 aacgacgctc attcgttaaa tcgcctcaag cgctgcaggc aataacaaat atcgtaatcg
     1081 ataaatttac tctacctttc atcactccat tgctccaccg ctatcaggat tgcttgttca
     1141 cgttgactct ctagatacca tcgagtagtt ttcaccaaat gatcatcggc gttcccaaag
     1201 agatcaaaac tgaagaaaat cgcgtcgctg taacgcccac cggggtcagc gggtttaccg
     1261 cccgcaatca caaggtcctg gttgaaaaag gcgccggtgt gggcagcgcg cttagcgatc
     1321 gcgcctatga gagcgcgggc gcgatcctca tcgattcagc aaaacaggtt tggagtgagt
     1381 ccgatctcat catgaaagtc aaagaacctc aggtatcgga gtttccgctg ctacggcccg
     1441 ggctcattct ctttacctat cttcatctcg ccgctaatga agcggtgact cgcgccctgc
     1501 tcgatcgcaa ggcaactgcc attgcttacg aaacgattca gctggaggat ggctcgttgc
     1561 cgctactgac gcccatgagc gaaatagccg gacggatttc cattcaggtg ggcgcgtggt
     1621 gcctgcaggc agaaaacggc gggcgcggcg ttcttctcgg tggcgcttcc ggggtccgac
     1681 ccggtaaagt tgtcatcctt ggcgcgggca ttgccggcac ggcagcctgc caggtagcta
     1741 tcgggatggg tgcccacgtc agcattctcg acattaatcc gacaaaactg cgctacgttc
     1801 gtgatatact cggcggccaa gctacgacct tgatgtccaa ccgcgccaac gtcgaggagg
     1861 aagttcttga tgccgatctg gtggtcggct cggtgctgat tcccggggct aaagcgccca
     1921 agctgatctc gcgattattg gtgcagcgca tgaagcccgg ggctgccttt gtcgatatag
     1981 ccatcgatca gggcggctgt gctgaaacat cacggccaac aacgcaccgc cagcccatct
     2041 atgtcgaaga agaagttgtc cattattgtg tgaccaacat gccggccatc gttccgcaca
     2101 cttcaacgta tgcgctgacc aactccacac tctcgtacgg cctggagctc gccaaccgcg
     2161 gctttccgca agcgctggcg cgcaaccgag cattggccaa aggtctcaac acgtttgccg
     2221 gcaaggtgac gcatgaagga gtggcagccg ccttcaatct tccttcattg cccttggagg
     2281 aggttattcg cgcatgagat tagtcacccg ggctgacttc gatggactgg tctgcggcgc
     2341 gctgctgacg aaattcgaga gcatcaatgc ctatctattc gtcgagccga agttcatgca
     2401 ggatggcttg gtcaaaatcc gcgctggaga catcatcgcc aatctgccct accatcccgc
     2461 ctgtacgctc tggttcgacc atcatgtcac taacacgacc ccggactttg ccaagccgat
     2521 tgtgctcggc agagggggct ttcgtctggc gccgagcgcc gcgcgcgtcg tctacgagta
     2581 ctataaagag ctaagcaata aaccggtagg cgaagagaaa accacgcgaa ttaacaatca
     2641 cgaaataatc gagttcctgg acagcgaaag aataaagtat ctgctccatg aaaccgatcg
     2701 aattgacgcc ggcaagctgg agccgcagga cgtccttgat cctcatgggt atgtgctggt
     2761 ttccatgaca accgatggac gaaatgccgg cgatgagccc tattggctgc ggatcatcga
     2821 actgctgcgc gatgcttccc tagaagaagt cctgcgtgac ccggaagtca gccggcgttg
     2881 ccagcggatc cgcgatgagc aagagcagct acgaaagctt ttgctggcgc gaacaaccat
     2941 gaagagaaac gtcatctatg tggacctgcg cggcatccaa gaaatcccgg atggcaaccg
     3001 gttcttgatt tttactctgt tcccgcaagg caatattcaa gtcaaggtcg cggacgacaa
     3061 acagcgtgaa aacaccacga gcatttccgt cggctacaat atattcaata ctacgtccaa
     3121 cgttaacgtc ggcgaactgt tgagtcatta tgggggcggc gggcacaaag tcgtcggctc
     3181 ctgccgcgtt cccaacgatc aagcagagac ggcgatccgc gaaatcgtcg ccgccgtgac
     3241 cgattagaag acaaccgccg agaatttggc agatgcgcgt gtcgcttttc aattttcgag
     3301 ttctgctacg cgcgtttctg ctcgcattgc ctatcatcaa cgtttgatga aaatcgtcaa
     3361 gatagcggcg gcgctcaagc gtttaaaaaa gattaacgat gaaatccggt acttgagctg
     3421 tttcgaacag aaacgcttca ccagcgggtt gatcgctttc aaaccgacaa agaaagcgaa
     3481 tccaaagcag atcagccacg accatcagga cgtcatttgc caggtgttac agggctccgg
     3541 cagactgcga atcgatggcc ggagaattcc gctcgctccc ggcatcctct gtcacatccc
     3601 caaaaggacg ccgcacgatt tcgccgccgc caggagcgga gaactggtgc ttttgtattt
     3661 gttaatcaag acgtagttgc cgacaccgag ctggtcctcc aaattcggag cccgcgagct
     3721 gtcgccgcat cgtcgtgcaa ttgtaagttt cgcagctatt tcttaggaca gcatcatcca
     3781 ttgataccgg acaatccggc cgggagcgat gtctgtcgag cgacttccgg aaagctgaaa
     3841 actctgtccc gcttctacct ggcgcggcgc atcgagaact gctgtgggtt tttgcgaatc
     3901 acgaacgacg acttcgacta ttgccggggt tgagatgttg ccggcatcat ccgtcactgt
     3961 cagctgaaat cggtgttgcc cgggcggcag cggcttgtct cgggtgacag ttacttctac
     4021 tactggagtt tcggtctcaa tcggctggcc tacgacaaac tgaggcatgt atctattctc
     4081 ctttctgctt tcacggcttt gccataactg agcggaagac gaaacaaaca tccgtcaact
     4141 gcgacacggc cgacggatca ggcacggact ttacgggttt acgcccgcta acgaggccaa
     4201 tctttcctca ttgacgaaca tacttggcct tcagaaagcc gtcccagacc gccagcgccg
     4261 gcttgggcct tccggtgctt gtctgcatgc cggtgaatgc ccagatgcgc gcgagatcgt
     4321 ccagcggcgg gcgcaaacgg ttgacgagcc ggtcggagtc ggtgctggca aaattgataa
     4381 cgaactcata tttatcgcgc gccgcggttt tcagcagcaa ctcggtaaaa tctctttgtt
     4441 cggcttccga acctaaaaga ctgagaccgt aacttttcag cggcacatcg cgcgatgtca
     4501 tgccactctc ggagaccgcg atcggcttct tgaatcgccg ggcgaaatcc agaaaatggt
     4561 cgggcagcgg gcgcggggtc tccaggctca tataaggata gaggctcatg acgaagagat
     4621 cactgtggcg cattagttcg gcgacttcct tctcctgctc cgattgcttg gcgtcgcgcg
     4681 ccaggcgctt gtaatgcaaa acctcggtcg taaaaaatac cggcaatttg ggatacattt
     4741 tcttaacggc gcggtaagtc tcgcggtgca attctttcaa ctgccgccac ttggcgctgt
     4801 cgcgagagag cagcacgttt gattcaacac cgatagccag a
//
LOCUS       DASUTG010000689         4792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_699969, whole genome shotgun sequence.
ACCESSION   DASUTG010000689 DASUTG010000000
VERSION     DASUTG010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699969"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1795)
                     /locus_tag="VF089_15920"
     CDS             complement(284..1795)
                     /locus_tag="VF089_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009401392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6175495.1"
                     /translation="MDLLANVALGFSVALTPANLMFCFLGSMVGTLVGVLPGLGPVGA
                     VAFLLSLTFKMEPASAVIMLAGIYYGAMYGGSTTSILVNIPGETASVVTCLDGYRMAR
                     QGRAGAALGISAFGSFIAGTLGIIGLMCLAPLLGRAALSFGPPEYFALTLTGLTLVSY
                     LSQGSMTKAMIMTILGLLAGSIGMDPISGAERFTFGILTLRDGLGLVPVAMGLFGIAE
                     VLENLTAIGQHAVYEAKIKGLLPNRKDWKDSLWPIARGSGLGFFLGILPGPGPVIASF
                     VSYAVEKRVSRYPEKFGSGVIEGVAGPEAANNAATASSFIPLLSLGIPTSAIMALFMG
                     ALMIHGIQPGPLFIARYPELFWGFVASMYVGNAMLLVLNLPLIGMWVRLLKTPYIILF
                     PLILLFCLIGVYSLNTNVAEIIIMLIFGVIGFVFRRVGFEGAPFILAMVLGPIMETSL
                     RQSLLISRGNFSIFFTRPICAVLMVVTVAFLISPLVTGKNKAAKLPSFKPGAL"
     gene            complement(1797..2252)
                     /locus_tag="VF089_15925"
     CDS             complement(1797..2252)
                     /locus_tag="VF089_15925"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6175496.1"
                     /translation="MRRLTIAASLILAGLAGLTLFEASRLAFGSIRVPQTGFFPSILA
                     ILLLFFSIALLVQTCRQAGGESGEQPITSEAWIRISITLAAMLGFALVLEKLGFLLST
                     FTVMLVLLRVIEPQKWPRVLAVALATALISYFLFGRLLNIPLPAGILEI"
     gene            complement(2283..3266)
                     /locus_tag="VF089_15930"
     CDS             complement(2283..3266)
                     /locus_tag="VF089_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6175497.1"
                     /translation="MMKNLRAVSLAQRRYLFLVLILLCAPALDAAEYPAKTVQIINPF
                     PPGAVTDIVARILAPRMSAALGQPVVIVNKAGGGGAVGIQAAKEAAPDGYTILVTPPP
                     ILLIPLVNKNSGFTMKDFTALALATSSPNTTVVRKESPWKTLEEFIAEAKKTPGQLTY
                     GSAGPGTTPHFIGELVKMKTGIDITHVPLGSESAAATSLLGGHVNIAFLTLGTTRSHI
                     EAGTLRALAVASNRRLKDFPDVPTTVEKGFPELNLKIWVGFFAPAKTPAAIVKRLAAV
                     INESLKDSEVAANIEKSQALIENFGPQEAAKFFVEEERKWSEVARVAKIAH"
     gene            3442..3936
                     /locus_tag="VF089_15935"
     CDS             3442..3936
                     /locus_tag="VF089_15935"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6175498.1"
                     /translation="MGTLAGAQQILGGLKQAGIDLVSSVPDINMLELINLLYEDKEIA
                     HVPVGREEEGIGVCAGAHLAGKRPAMLMQNGGLLNSCNGLTTTALQFGIPVLLLIYYA
                     GDIGDNAFHMVGLVTEPVLQGLGIKYIVMRDPAKVKQEIAGAVTLANSSKRPVALLLT
                     RAVL"
     gene            3947..4561
                     /locus_tag="VF089_15940"
     CDS             3947..4561
                     /locus_tag="VF089_15940"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6175499.1"
                     /translation="MKRYECLQTIAPLIKDELFVTTAGGATAEWNGVRPSDGNIQVKT
                     LGLCSSIGLGIALSLPKRKVFVFDGDGALWMNLGSLATIGLHQPKNLIHICWDNKQYE
                     SSGGEPTVATAGRIDFAGVARTAGIQSSRAVATIEELKEAVTHALSHDGPHFICATIE
                     AGRTEAPPLRYDELENKYRFVRYIEETEGINILRVPMPASYQLK"
     gene            complement(4588..>4792)
                     /locus_tag="VF089_15945"
     CDS             complement(4588..>4792)
                     /locus_tag="VF089_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175500.1"
                     /translation="LIRIGYGCINTKLTSPNRTCRLKNATPDKILKLAAAMPKRCSPS
                     WNRTGPGHQTLPHHVELISFDPQ"
BASE COUNT         1120 a         1337 c         1381 g          954 t
ORIGIN      
        1 ggctcaaccg cgatattcgg cttgtttgaa gagaacgcta aaacgaacgg gtaaatgttg
       61 gcttgcgaaa aagctggcaa accaacctac acggctggac caacggcaag cgtaagcacg
      121 gccgtctgac acgacaccag cgcgccgttc aactcccatg cgtcgctccg ctcggtgttt
      181 cgattcgacg agcctgtttg cagttgcgca attcgaactg accgataaac ctgtggatga
      241 atgcagcggg ggtggggtcg gtcatgtttg agttactcct tttctacaat gcgcccggct
      301 tgaacgatgg aagtttggcc gctttattct ttccggtcac gagaggtgaa attaaaaaag
      361 ccaccgtgac caccatcagg acggcgcaga taggccgggt gaaaaagatg ctgaaattgc
      421 cgcgcgagat taacagagat tgtcgcagag aagtctccat gattggaccc agcaccatcg
      481 ccaggatgaa aggcgcgcct tcgaatccga ctcggcgaaa gacgaagccg ataacgccga
      541 agatcagcat gatgatgatc tccgccacgt tggtattcaa gctataaacg ccgatgaggc
      601 agaacaacag gatgagcggg aacagtatga tatagggcgt cttgagcagc cgcacccaca
      661 taccgatcaa tggcagattc agcaccagca gcatggcatt gccgacgtac atgctggcga
      721 cgaagcccca gaaaagctcc ggatatctag cgataaacaa aggtcccggt tgaatgccgt
      781 ggatcatcag cgcgcccata aaaagcgcca tgatggcgct ggtcgggatt cccagtgata
      841 gcaacggaat gaacgagctg gcagtggcgg cattgttggc ggcctcagga ccggcgactc
      901 cttcgataac accgctgccg aatttctccg gatagcgcga cactcgcttc tctactgcgt
      961 aagacacgaa cgaggcgatc accggtccgg gaccgggcag gatgccgaga aagaagccca
     1021 agcccgaccc gcgcgcgatt ggccagaggc tgtccttcca atctttgcgg ttgggcagca
     1081 ggcccttgat cttggcttca taaaccgcat gctgaccgat ggccgtcagg ttctccagca
     1141 cttcggcgat accgaacaat cccatggcca cgggcacgag tccgaggccg tcgcgcaacg
     1201 tgagaatgcc aaaggtaaag cgctctgcgc ccgatatcgg gtccatgccg atgctgcccg
     1261 cgagcagacc caggatcgtc atgatcatgg ccttggtcat ggaaccctgc gacaagtacg
     1321 ataccagggt taaaccggtg agcgtcagag caaaatattc cggcggtcca aaactcaatg
     1381 ccgctctgcc gagtagcggc gccaaacaca tcaggccgat gataccgagc gtgccggcga
     1441 taaaggagcc gaaggcggaa attcccagcg ccgccccggc ccgaccctgc cgcgccatgc
     1501 gataaccatc aagacaggtg acgaccgaag cggtctcccc cgggatattt accaggatcg
     1561 atgtcgtgga accgccgtac atggcgccgt agtagatacc cgcgagcatg atcacggcgc
     1621 ttgccggttc cattttaaaa gtgagcgaca gcaggaacgc cacggcgccc accggaccca
     1681 aacccggcag cacgccgacc aaggtgccga ccatcgatcc aagaaaacaa aacatcaagt
     1741 tggccggggt gagcgcgacg gaaaatccga gcgcgacatt ggcaaggagg tccaccttag
     1801 atttccagga ttccggcggg tagtggaatg ttgagcaatc ggccaaagag aaagtaagag
     1861 atcagcgcgg tcgcgagcgc tactgcaagc accctcggcc acttttgcgg ttcgatcacc
     1921 cgcagcagca caagcatcac agtgaatgtg ctcagcagaa atcccagctt ctcgagcacc
     1981 agcgcaaagc cgagcatcgc cgccaaggta atgctgattc taatccaggc ttcactcgtg
     2041 atcggttgct cgccgctttc gccaccggct tgacggcaag tctgaaccag caatgcgata
     2101 gaaaaaaaca gcagtaggat agccaaaatc gaagggaaaa aacccgtctg aggtaccctg
     2161 atgctgccga aggcgagtcg gcttgcttcg aacagtgtca atccagccag tccggctaga
     2221 atcaagctgg cggcgatggt gagtcgtctc atcgacggcg cggaaaaatt ttttagcttc
     2281 gctcaatgag cgatcttcgc aactcgcgca acttcggacc atttgcgctc ttcctcgacg
     2341 aagaacttgg cggcttcctg aggtccgaaa ttttcgatga gggcctggct tttctcgatg
     2401 ttggcggcga cctcggagtc cttgaggctt tcattaataa ccgcggcgag ccgtttgacg
     2461 atcgctgccg gagttttggc cggggcgaag aagcccaccc agatcttcaa gttgagctcc
     2521 ggaaaacctt tctccactgt ggtcggcaca tcgggaaagt ccttgaggcg gcgattggaa
     2581 gccactgcca acgctctcag cgtcccggct tctatatgac ttcgggtcgt gcccagggtg
     2641 agaaacgcaa tattgacatg cccgcctaac agcgatgtgg cggccgccga ttcggatcca
     2701 agcgggacat gcgtgatgtc gatgcccgtc ttcattttca ccagctcgcc gatgaagtga
     2761 ggcgtggttc ccgggccagc cgagccgtag gtgagctggc cgggagtttt tttcgcttcg
     2821 gcgatgaact cctcgagagt tttccagggc gattcctttc gcaccactgt tgtgttgggg
     2881 ctgctggtcg ccagcgctag cgccgtgaaa tccttcatcg taaagccgct gttcttgtta
     2941 accagcggaa tcagcagaat cggcggcggc gtcacaagaa tcgtgtagcc gtcaggggcc
     3001 gcctctttgg cagcctggat acccaccgcg ccgccgcccc cggctttatt gacgatcacc
     3061 accggctgcc ccagagcagc cgacattcta ggggcgagaa tgcgcgccac gatatcggtg
     3121 acggcgccgg gcggaaaagg attgatgatc tgcacggtct tggcggggta ttcggcagcg
     3181 tccagcgccg gcgcgcaaag cagaatcaag accagaaaca aatagcgcct ttgtgctagc
     3241 gaaaccgcgc gcaaattttt catcatctgt gctcccaacg gctaatatcg ctctctctgc
     3301 taacagaatc gccggtggag tgtcaaacat tctgtgcacc cgagtcttcg aaaattattc
     3361 gagaatcaaa cggcccgatc gcagttgaca gggataacag aaatacttta gaagtcagac
     3421 aatgcaaggg tgacatcgac tatgggaaca ttagcaggag cgcagcaaat tctcggcggg
     3481 cttaaacagg ccggcatcga tctggtgtcg agcgttcccg acatcaacat gctcgagctg
     3541 atcaatctcc tttatgaaga taaggaaatc gctcatgttc cagtgggccg tgaagaagag
     3601 gggatcggcg tttgcgccgg cgcgcatctc gccggcaagc gcccggctat gttgatgcaa
     3661 aacggcggct tacttaatag ctgcaacggt ctcaccacca cggcattgca atttggcata
     3721 ccggttttgt tgctgatcta ttacgcgggc gacatcggcg ataacgcttt ccatatggtg
     3781 ggactcgtga ccgaaccggt gctgcaggga ttgggcatca aatacatcgt catgcgcgat
     3841 cccgccaaag tgaaacaaga aatcgccggc gccgtgaccc tggccaatag ctctaagcga
     3901 cctgtcgcat tgctgctgac ccgggcggtt ttgtaatcag gactttatga agcgttacga
     3961 atgtcttcaa accatcgctc ccttgatcaa agacgagctg tttgtcacca ccgccggcgg
     4021 cgccacggcg gaatggaacg gggtgaggcc cagcgacggc aacatccagg tgaaaactct
     4081 cggtctgtgc tcttctatcg gcctgggaat cgcgctttct ctcccgaagc gtaaggtttt
     4141 tgtcttcgac ggcgacggcg ccctgtggat gaatctggga tcgctggcga ccatcggcct
     4201 gcatcaaccg aaaaatctca ttcacatttg ttgggacaac aagcagtacg agtcttccgg
     4261 cggggagccg acggtcgcta ctgcggggag aatcgatttc gccggcgtgg cgcgcaccgc
     4321 cggcatccag agctcgcggg cggtagccac catcgaggag ctgaaggagg ccgtgactca
     4381 tgcgttgagc catgacggtc cgcattttat ttgcgcgact atcgaagccg gccgaaccga
     4441 agcgccgccg ctgcgctacg atgagctgga gaacaagtac cgctttgttc gttatatcga
     4501 ggaaacggaa ggcattaata tcctgcgtgt ccccatgccc gccagttatc aactgaaata
     4561 gtgaacgccg tctagccgtg ttgtcggtca ctgaggatca aacgagatca gttcgacgtg
     4621 atgcggaaga gtttgatgtc ctgggcccgt tctattccag gatgggctgc agcgcttcgg
     4681 cattgcagcg gcgagcttaa ggatcttgtc aggcgtagcg ttctttagcc ggcaggtgcg
     4741 attgggcgag gtcagcttag tgttgatgca accgtaaccg atgcggatca aa
//
LOCUS       DASUTG010000690         6121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2183137, whole genome shotgun sequence.
ACCESSION   DASUTG010000690 DASUTG010000000
VERSION     DASUTG010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183137"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF089_15950"
     CDS             complement(<1..304)
                     /locus_tag="VF089_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6175501.1"
                     /translation="MAEVKDFKTVKVEKDQGIAWVILNRPEKRNAMNPQLHYDMLEAL
                     TELEVDKDSKVVVITGAGPSWCAGQDLREYFRGTDTDPVERRKASWASQEWRWRRLF"
     gene            complement(385..1380)
                     /locus_tag="VF089_15955"
     CDS             complement(385..1380)
                     /locus_tag="VF089_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175502.1"
                     /translation="MNRKTFVCAITVFFLCIGWRAMAQDKIRMGLSSVSGLHTAVWVA
                     ETKGLFRKHGIDPEVIVTGQGGTAGISALIANDVQMVNSAGDVLVAASLRGGDTVMVA
                     SVVNKGLQRLITKPDIKTPEELKGKRVGVTRIGAVSHSVLLMMLQRWKMSVNDVQILQ
                     LGSSPSMLASLDKGGIEGAVVTIPSMFVAEDRGYRVLLDMADTDIYYLHTMVATTRQY
                     IKNNRDKVVRFLKGYVEGIAFVKHNKQESLDIVKKKLRIGPEQEKNLERSIDLLTAKY
                     YESVPYPSLRGVETVLGFIEKDNPKAKGADPKMFVDDSLLREIEASGFVKTLYQR"
     gene            complement(1554..2621)
                     /locus_tag="VF089_15960"
     CDS             complement(1554..2621)
                     /locus_tag="VF089_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175503.1"
                     /translation="MTTLTSPIIEVEDSWEAANDWFCRERLSDGLPIVPPTPERVTRM
                     LEGTGRDPQEVIGLIPPKWAPASIEKLAINAVMAGCLPEYLPVIIAAVEAICEPRFNL
                     YGVQATTGYVGPALLLNGPIRTALNVNCDSGVFGPGFRANATIGRAIRLILITVGGGY
                     PGDTDRSTFGWPGKYTMCFGENQEKSPWEPYHVEMGFRADDSTVTVFGINGFLPIHTA
                     GNRGEQALSSLAEVIKMHHGVSHLDVGSFGGGTPLIGLGIEDAEIMARDGITKKQVKD
                     YLWEHASLKFTEVPARRKGHKGDDELLRESPNVTPDGVVHLSTKAEDIMVVVLGGKHR
                     HSVFLPMWTGRNTLSVIKPIR"
     gene            complement(2657..2902)
                     /locus_tag="VF089_15965"
     CDS             complement(2657..2902)
                     /locus_tag="VF089_15965"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX6175504.1"
                     /translation="MQRRLIIELDGGQHAQEANHDAVRDAWLREQGFIILRFWNNDVL
                     KNMDGVMTKIRTKLQSTPYLNPSPQGGRKKMQSRNRP"
     gene            complement(2881..3054)
                     /locus_tag="VF089_15970"
     CDS             complement(2881..3054)
                     /locus_tag="VF089_15970"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX6175505.1"
                     /translation="MNPTRSRARQLRRNPTDVERLLWQRLRFWQVDDYKFRRQQPLGV
                     YIADLRVCKDDSS"
     gene            complement(3146..3322)
                     /locus_tag="VF089_15975"
     CDS             complement(3146..3322)
                     /locus_tag="VF089_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175506.1"
                     /translation="MELAKTEAKFFGLATLPLVEIPHHPSGGARAGEHRADAEGAVDL
                     VRQRLTLGFSTLRS"
     gene            complement(3410..3697)
                     /locus_tag="VF089_15980"
     CDS             complement(3410..3697)
                     /locus_tag="VF089_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175507.1"
                     /translation="MATLKMLDPRAGVNPEDRPVMPGLDSLEGKVIGIIDNGQSNSTA
                     MFQELAKLMREKLLTAEVLFKTKPSHMQGAPGPIIEEFVSRCDAIITGLGA"
     gene            complement(4149..4709)
                     /locus_tag="VF089_15985"
     CDS             complement(4149..4709)
                     /locus_tag="VF089_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX6175508.1"
                     /translation="MGHGVRHRPNAFNYQEMLPKLKDLSALARRVGVRVFYSVQTGFD
                     LAKEEAGVWVRIRMKRAKASDPSELLKQKDDPHGRDIIAELKPEPQDIVFQKRRPDAF
                     VGTDFDLMLRSNGIKTIVISGVATEGGVEGTARSARNLGYEAVTVKDCVGSRSRELHE
                     MALKLMEGSFFDVTTAVELAAIWGEK"
     gene            complement(4938..6029)
                     /locus_tag="VF089_15990"
     CDS             complement(4938..6029)
                     /locus_tag="VF089_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175509.1"
                     /translation="MASNPKNSVAQFSGEIGIHFPLHVLNRYSLPDLIALAERSRDTL
                     GPHGFTQVWTNDNLEYRSVLASSAALVARLSIKLGTAIAVPYFRNPIDFAAAFATMSE
                     LTDGQEISLGLGPGSRSILTHHVERIKPMTIMAEIAIALRTLFKGDAIHRSDMPALAD
                     YFHLNAESYTLRFKTRGPIRLYYGPSLLKPGVLKLIARQFDGVILQTLYGITAMEETL
                     NTLADARKHSALGEPLRKVMLLNASVSRDGQAAKQHAKRFVSHIASGWPDDTLVRIGI
                     DPQTMEPVRRAYAQNLGIDYAAAITPDDAVERLIISGTPAQCRERIAELFAFAARHEF
                     FQVCIGVPAGPDIAEVIDLWGKEILPALR"
BASE COUNT         1303 a         1753 c         1593 g         1472 t
ORIGIN      
        1 taaatagccg gcgccagcgc cactcctgag aggcccagct ggcttttcgc ctttccaccg
       61 ggtcggtatc ggtgcccctg aaatattcgc gcaggtcctg accggcgcac cagctcggac
      121 cagcgccggt gatgacgacg actttgctgt ctttgtcgac ttccagttcg gtaagcgcct
      181 cgagcatgtc gtagtgcagc tggggattca tcgcattgcg cttctcggga cgattaagaa
      241 tgacccaggc aatgccctgg tccttttcaa ctttgacggt tttgaaatcc tttacttctg
      301 ccattgttct ttcctcctta tgttctctcg ttgatctttt tgtgttgttc aggtgacgct
      361 gttttccggt ccatatcaag tcgcctaacg ttggtaaagt gtttttacga agccgctcgc
      421 ctcgatctcg cgcagcaggc tatcatcgac aaacattttg gggtccgcgc ctttggcttt
      481 cggattgtcc ttctcgatga agccgagcac cgtttcgacg ccgcgcaatg acggatatgg
      541 cacagactcg tagtatttcg cggttaacag atctatggag cgttcgaggt ttttttcctg
      601 ctcgggaccg atgcgaagct tcttcttgac gatgtccaaa ctctcctgct tgttatgctt
      661 gacgaacgca attccttcga cgtaaccctt gagaaatcgc accactttgt cgcgattgtt
      721 cttgatgtat tggcgcgttg tcgcgaccat cgtgtgcagg tagtaaatat ccgtatccgc
      781 catgtcgagc aacactctat agccgcgatc ttcggcgacg aacatcgaag gaatcgtcac
      841 cacggctcct tcgatacctc ccttgtcgag gctcgccagc atactcggcg aggaaccgag
      901 ttgaaggatc tggacatcat tcacgctcat cttccaacgc tgcagcatca tgagcagaac
      961 cgaatgggaa accgcgccga tgcgagtgac gccaacccgc tttcccttca gctcctcggg
     1021 cgtttttatg tccggcttgg taataagccg ttgcagtcct ttgttgacaa cactggcgac
     1081 catgaccgta tcgccgccgc gcaacgaagc tgcgaccaag acatcaccgg ctgaattcac
     1141 catctgcacg tcgttggcga tgagcgcact gataccggcg gtcccaccct ggccggtgac
     1201 gatcacttca ggatcgatgc catgtttgcg aaagagtcct tttgtttcag ccacccacac
     1261 agcggtgtgc aaaccgctca ccgagctcaa ccccattcta attttgtcct gcgccatggc
     1321 acgccaaccg atgcaaagaa aaaaaacagt tatggcgcat acgaatgttt ttcgattcat
     1381 cgctacggtc ctccgtttag ctctcgccag tttcaccagt gcctgatgcc gataagcagg
     1441 gtcgatatac ttcggggttt atagcctgtc catcatcgca ttggctatat agagaaaatc
     1501 ggcggtctct tgaatgtgag cggcgagccg ctccgtcgct ccagcgcccg ttatcatcga
     1561 atcggtttaa tcaccgataa agtattccgg ccggtccaca tcggcaagaa aaccgaatgg
     1621 cggtgcttgc cgccgagcac cacgaccata atatcttcgg ctttggtcga caggtgaacg
     1681 accccgtccg gcgtgacgtt tggcgattcg cgcagcagct catcatcacc tttatgtccc
     1741 ttgcgccgtg cgggaacttc agtaaatttc aacgacgcat gctcccagag ataatcctta
     1801 acttgcttct tggtgatgcc gtcccgagcc atgatctccg cgtcttcgat gccgaggccg
     1861 atgagcggcg tgccgccgcc gaaactgccg acatcgagat ggctgacgcc gtgatgcatc
     1921 tttatgacct cggccaaaga cgagagcgcc tgctcgccgc gattgccagc cgtgtgaatc
     1981 ggcaggaatc cattgatgcc gaagacggtc acggtactgt catcagctct aaaacccatt
     2041 tcgacgtgat acggctccca tgggcttttc tcctgattct caccgaagca catggtgtac
     2101 ttgcctggcc aaccgaaagt cgatcgatcg gtgtcgccgg ggtaacctcc gccgaccgta
     2161 atgagtatca accgaatcgc acggccgatg gtcgcattgg ctcgaaaacc gggcccaaac
     2221 acgccggagt cgcaattgac gttgagcgcg gtcctgatcg gaccgttgag aagcagcgcc
     2281 ggaccgacgt aaccggtggt tgcctgcacg ccgtagaggt tgaaccgggg ctcgcagatc
     2341 gcttcgacag cggcgataat cacgggcaag tattccggca ggcatccggc catgacggcg
     2401 ttgatggcga gcttttctat gcttgccggc gcccacttgg gtgggatgag cccgatcact
     2461 tcctgtggat cacgcccggt accctcgagc attcgcgtta cccgctccgg ggttggcggc
     2521 acgatcggca agccatcgct cagcctttcg cggcagaacc aatcgttggc cgcttcccaa
     2581 ctgtcttcga cttcgatgat gggtgaggtg agtgttgtca tcagagttaa tgccaattct
     2641 gctcaaagcg agctcatcaa ggacggttgc ggctttgcat cttcttcctt cccccttgcg
     2701 gggaaggatt gaggtaggga gtgctttgta gcttggtacg aatcttcgtc attactccat
     2761 ccatattctt gagcacatcg ttattccaaa acctcaaaat gatgaaaccc tgctcgcgca
     2821 accaagcatc ccgcacggca tcatggttcg cttcttgagc gtgttgtccg ccatcgagtt
     2881 ctatgatgag tcgtctttgc aaacacgcaa atctgcgatg taaacgccca aaggctgttg
     2941 gcgccgaaat ttgtaatcat ccacttgcca gaaacgaagc ctctgccata gtagccgctc
     3001 gacatctgtt gggtttcgcc gtagttgccg cgctctcgag cgtgttgggt tcatacccta
     3061 tccttgcctt tcccccttgc ggggaaagga atcactcaaa acagttggtt gatcaaattc
     3121 ttgcgttcgg cacagcttaa gtgatctagc tgcgcaaggt gctaaatccc aatgtcagcc
     3181 gctggcgcac caaatccacc gcgccttcgg catcggcgcg atgctcgccg gctctggcgc
     3241 cgccgctcgg atgatgcgga atttccacca gtggaagtgt cgccagtccg aagaacttcg
     3301 cttcagtctt ggccagctcc atgaaaatcg attgacacag agtgactgtt ggctttcctt
     3361 gcttttgcag ctcgatcgca tcgtggacac tccacgatgt gcacgaccct caagctccga
     3421 gaccggtgat gatcgcgtca caccggctga cgaattcctc tataatcggc ccaggcgctc
     3481 cctgcatatg cgaaggcttg gtcttgaaaa gcacttccgc tgttagaagt ttttcccgca
     3541 tcaacttcgc caattcttga aacatcgccg tggaattgga ttggccattg tcgataatgc
     3601 cgatcacctt cccttctaga gaatccaagc ccggcatcac gggccggtct tcgggattta
     3661 caccggctct cgggtcaagc atcttgagcg ttgccattgc actgcatcct cctgcaacat
     3721 gaccaagtat tttgtaattc ttatacaaga ttcgaagaga aatttttcag tcgttcgttc
     3781 cttatatgca agtcatccat tcgctgcaat actacttcag caccggcacc taatttcgcg
     3841 gacatcccgc ctacattgag aataagcaat tggtcaatcg cttgacccgg agttcacctc
     3901 aagtaggaaa tgcgccgcta ttcaaagtct agtgaggagc tatttgagat gcgacgggag
     3961 aatagctgtg ccgaggattt cttaaccgtc gtattgcaac aagctcaatc ttccggattt
     4021 ctcgtgcgcg ttgccaataa ggtttcgcga gcggtgtcgt atgttgatgg atgatcgtga
     4081 ctggtgaggc ggatgtgttc ggtcattggc cgttgagtac ctatgtgtca ttcagccgcc
     4141 ccactatctc atttttctcc ccaaatcgcg gcgagttcga cggcggtggt gacatcgaaa
     4201 aagcttccct ccatgagttt cagcgccatc tcatgcaatt cgcgacttct agagccgacg
     4261 cagtctttca cagtcaccgc ctcgtacccg agattgcgcg cgcttcgcgc cgtaccttcg
     4321 acaccgcctt ccgtggcaac gccgctaatc acaatcgttt tgatgccatt actcctgagc
     4381 atgagatcga agtcggtccc gacaaatgcg tccggccggc gtttctgaaa cacgatatct
     4441 tgaggctcgg gtttcaactc cgcgatgatg tccctgccgt gcggatcgtc cttctgtttt
     4501 aaaagctcgc tcggatcact ggctttcgcc cgcttcatcc tgatgcgcac ccaaactccc
     4561 gcttcttctt tggccaagtc gaagccggtc tgcaccgaat agaaaacccg cacgccgacc
     4621 cggcgagcca gggcggacaa gtccttcagc ttgggcagca tttcctggta attgaaagca
     4681 ttcgggcgat ggcgtactcc atgtcccata tgagcaaagc tgtctgggag ggttcgacaa
     4741 tttccgccaa cgtgctgggg attttctttc cggcgatctc atacatgatt cacctcgctg
     4801 tattcttcta tcaaaacatc gtcggtggtt actcgaaagc gaagctcgtc gccggcaaac
     4861 cgtgcgcagg aaatcaccgt aaagctcgac ggcgcaccaa gccgataaat ccgaattgtg
     4921 gctttggtca cccgttgcta gcgcagcgcc ggcaggatct ccttacccca caggtcgatg
     4981 acttccgcga tatccggtcc cgccggcacg ccgatgcaca cctgaaaaaa ctcatgccgc
     5041 gccgcaaagg caaaaagctc ggcgatgcgt tcgcgacact gcgccggtgt tccggagata
     5101 atcaatcgct cgaccgcatc atccggagtg atcgccgcgg cataatcgat gccgagattc
     5161 tgcgcatagg cccgacgcac cggttccatg gtttgcgggt caatgccgat gcgtaccagc
     5221 gtatcgtccg gccaacccga agcaatatgc gaaacaaaac gtttggcatg ctgcttcgcc
     5281 gcctgtccat cgcgggacac agaggcatta agcagcatga cttttcgcag cggctcaccg
     5341 agagccgagt gcttgcgcgc atcggcaaga gtattcaacg tttcttccat agccgtgatg
     5401 ccgtagaggg tttgcaggat gacgccgtcg aactggcgcg cgatcagctt caacacgcct
     5461 ggcttgagca ggctgggacc ataatagaga cggatcggcc cgcgggtttt gaagcgcaac
     5521 gtgtagcttt cagcgttcag atgaaaatag tcggccagcg caggcatatc gctgcggtgg
     5581 atcgcatcac ctttgaagag cgtccgtagc gcgatcgcga tttcggccat gatcgtcatc
     5641 ggtttaatcc gctcgacgtg atgggtcaga atagatctcg atccaggtcc taaaccgaga
     5701 gagatttctt gtccgtcggt cagctcggac atggtcgcaa acgccgcggc aaaatctatg
     5761 ggattacgga agtacggaac cgcaattgcc gtcccaagct ttatagagag ccgcgctacc
     5821 aatgccgctg agctcgccaa gacgctgcga tactccaaat tgtcattggt ccacacctga
     5881 gtaaagccgt gcggcccgag agtatcgcgc gagcgttccg ctaatgcgat aaggtcgggc
     5941 agagagtaac ggtttagaac gtggagcgga aaatggatac ctatttcgcc gctgaactgt
     6001 gcaactgaat ttttaggatt acttgccatg atgggcgagg gctatcacgg caggtgaaaa
     6061 agagtcaatg gagatgtgac agatgcggct cgcgtattcg gcagaagatt atcctcgagc
     6121 a
//
LOCUS       DASUTG010000691         5712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1737030, whole genome shotgun sequence.
ACCESSION   DASUTG010000691 DASUTG010000000
VERSION     DASUTG010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737030"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VF089_15995"
     CDS             <1..1324
                     /locus_tag="VF089_15995"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX6175510.1"
                     /translation="WFIRPIIELVPADLACPQCGKSDFFREEDILDVWFDSGVSHAAV
                     VETDPRLGGRANLYLEGSDQHRGWFHTALLTSIATRHRAPYESVLTHGFTLDGKGRKM
                     SKSLGNVIAPQEITKKSGAEILRLWVAAEDYREDVRISDEILNRLVEAYRRLRNTARF
                     LISNLYDFDPEHDSVSPPDLDELDRWILHRTESLLSRCREAYEKYEFHTVFHALNNYC
                     SVDLSAQYLDIVKDRLYCAGAKSKERRAAQTTLDRILNVLVHLLAPILSFTAEEIWTY
                     MGAKDRLSSSIFLSKIPETDPTMLDRELADRWDRIFKERAEVLKALEEARTKGIIGHS
                     LDARVILVNQNGGPNSSLGNLIKSDRSKMQDILIVSQADISESMTSDSANLSWYKTEL
                     LNCQIAVLKAEGLKCERCWKYDTEVGRDTIHPTVCARCAAVLNSGAAV"
     gene            1321..1818
                     /gene="lspA"
                     /locus_tag="VF089_16000"
     CDS             1321..1818
                     /gene="lspA"
                     /locus_tag="VF089_16000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6175511.1"
                     /translation="MSRKTRIVAGCLGFIVIADQLTKYIVDRTMPLHHSIPVIDGFLS
                     LTYIRNTGAAFGMFSGSHEAFRLPFLIIVSLIAIGFIFTMLKRLRETETGLITALAFI
                     LGGAIGNLIDRMIHGEVVDFLDIYWSDYHWPAFNLADSFITMGVGITIYYLATIRGDD
                     PFAAK"
     gene            complement(1952..2245)
                     /locus_tag="VF089_16005"
     CDS             complement(1952..2245)
                     /locus_tag="VF089_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX6175512.1"
                     /translation="MSETHPPFDEPVVLPLGDVLDLHPFQPEDIPSVVEEFLVECRRS
                     GILELRLIHGKGAGVQRAIIRSLLDKHPAVISFKDAPSEAGGWGATVVNLMRE"
     gene            complement(2340..4775)
                     /locus_tag="VF089_16010"
     CDS             complement(2340..4775)
                     /locus_tag="VF089_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175513.1"
                     /translation="MDENFTKPLSPVFGPPAPAPLLFGSSGSAIYWLSITLLLFGVFL
                     ADLYSPFGVAIPTSYVAALLLVLALPGAREKILVALISTVLIILDYFLTPGSPAIPGW
                     IPIFNDALTVTLIWIVAGLGLRHRRAQDAMRRSEERFRALVEASSQIVWTADGAGNIV
                     EDSPSWRAFTGQTFEQRRGTGWLNAYHPNDHKRVQMQWEDAIMRKTPLEAQYRLRRHD
                     GQWRWTLVRAVPLLSADGSARGWVGMNTDITERKQAEADTLFLLDLSDCIRLASDSDE
                     LTWAVAVALGEYLQAERCSFIEADPERGRFTIHRDYHLNLPSLVGTYRLSALGPKIVS
                     DCQAGHNIVVFDTSRDGRTAIYSESYQRIGIGAFAATPLLREGRLVSALFLSTTEPRG
                     WSEREISLIAAAAERTWLAVERLRLNSALSQSEEALRDADRRKDEFLATLAHELRNPL
                     ALVRNVVSLSQASGFSEAQQRWGRDVIEKQVAYLTRLTDDLFDISRITREKLGLHKEH
                     VELCDIVKTAVESSRPLIEERGHEITITLPGSPVYVDADRVRLTQVVMNLINNAAKYT
                     PDPGQIWLALGLQNDHAVLQVKDTGIGIAPEDLPRLFDLFYQADRSFTRAEGGLGLGL
                     TLVQRILELHGGTVQAFSLGARQGSEFVVNLPVLRKPSQQKLSPDVDGNSPSIKSPIR
                     RILVADDFPQAAETLGGLLRQEGYEVQIAQDGLEAFQAAERSHPDVIVLDLAMPELSG
                     YDAARKIREQPWGKEVVLIALTGWGQQRDRHRTKEAGFDAHLTKPVKYEAILELLGNL
                     PNRQRPGSAAPTV"
     gene            5048..>5712
                     /locus_tag="VF089_16015"
     CDS             5048..>5712
                     /locus_tag="VF089_16015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175514.1"
                     /translation="MASRKYYRDFRDHLKELEARDKLIRIHRRIDKDTELMPLVRWQF
                     RGLDEEQRKAFLFENVVDAKGKQYSMPVTVGTLAATTEIYATGLMCRPEEIHERWNKA
                     QLSPIEPVKVGAAPVQEIVWTGKELLNGHGLDMLPVPISTPGFDNAPYLTSANWITKD
                     IESGVYNIGNYRSQIKSPDRAGGLFTSQHMGQHWRKCKAKGVPLQAAIAIGVIPSIAY
                     AATA"
BASE COUNT         1354 a         1473 c         1524 g         1361 t
ORIGIN      
        1 atggtttatc cggcctatca ttgaactggt gccggctgat cttgcctgtc cacagtgtgg
       61 caaaagcgat ttctttcgag aggaagatat tcttgacgtc tggtttgact cgggcgtgag
      121 ccatgcagca gtagtcgaaa ctgacccgag gctcggtggt cgcgccaatc tctatcttga
      181 aggaagcgat cagcatcgtg gttggtttca cactgcgtta ctgacctcga tagctactcg
      241 gcaccgcgca ccttatgaga gcgttcttac gcatggcttt accttggacg gtaagggacg
      301 caagatgtca aaatccttgg gcaatgtcat tgcgccacag gaaatcacta agaaatcggg
      361 cgcggaaatt ttgcggctct gggtggcagc tgaggattat cgcgaggacg tgcgcatctc
      421 agacgaaatt ctcaatcggt tggtggaggc gtatcggcga ctcagaaaca cggcgcgttt
      481 tttgatcagc aatctttacg atttcgatcc cgagcacgat tccgtttcgc cgcccgacct
      541 ggacgaactc gaccgttgga tcctgcatcg gaccgaaagc ttgctaagtc gttgccgaga
      601 agcttacgag aaatacgaat ttcatactgt gttccatgcg cttaataact attgtagcgt
      661 ggaccttagc gcgcagtacc tcgacatcgt gaaagaccgc ctttactgtg ccggtgcaaa
      721 aagtaaagag cggcgagcgg cgcagacgac gttagatcga attttgaacg tgctggtcca
      781 cttgctggca ccgattctct catttacggc cgaagagatt tggacctata tgggggcaaa
      841 agatcgtctg tccagcagta tctttctttc caaaataccg gaaacggatc ccaccatgtt
      901 ggatcgagaa cttgctgaca gatgggaccg tatcttcaaa gagcgcgccg aggtgctcaa
      961 ggctttggaa gaggcgcgaa ctaaaggcat tatcggtcat tcgctggatg caagagtcat
     1021 tttggttaat cagaacggcg ggcctaattc tagtctggga aatctgatca agtctgatag
     1081 gagcaagatg caggatattt tgatcgtctc acaggccgac atttctgaat cgatgacatc
     1141 tgatagtgcg aatttgtcgt ggtataaaac ggagcttttg aattgccaaa tcgcagtctt
     1201 aaaagccgag ggtcttaaat gtgagcgttg ctggaagtac gacacggagg tgggcagaga
     1261 tacaatccac ccgacggtct gtgcccgttg tgccgcagtt ttgaattccg gagccgcggt
     1321 ttgagtcgca agacaaggat cgtcgccggc tgcctcggtt tcatcgtaat cgccgatcaa
     1381 ctcacaaagt atatcgtcga tcggacgatg ccgctacatc actctattcc ggtcatcgat
     1441 ggatttttga gcctcacgta catccgcaat accggtgcgg cattcggtat gttttccggc
     1501 agtcatgaag catttcggtt gccgttcttg attattgttt cgctcatagc gatcggcttt
     1561 atctttacga tgttgaagcg cctccgggag actgagactg gtctcattac agcgctcgcc
     1621 tttattcttg gcggggccat cggcaatctc atcgaccgaa tgattcatgg cgaggttgtc
     1681 gattttttag acatatactg gtcggattac cactggccgg cgtttaattt ggccgacagt
     1741 tttataacca tgggtgtggg gatcacgatt tattatctcg ccacgatcag aggcgatgat
     1801 ccgtttgccg caaaatagct ctgctcttgg tcctctcgtt tctcaacctg tattcttttg
     1861 gcacgggcat aggaacaagt agcgaacctg aatgtactga acttagcgct aaggtgattc
     1921 gtagattcga attcggattc aaacccctat tctactcgcg catcaagttg acgacagtag
     1981 caccccaacc tccggcttca cttggggcat ctttgaaaga aattacagca ggatgcttat
     2041 cgaggagtga tcggatgatc gcgcgttgca cgcctgcgcc tttgccatga atgagacgca
     2101 attccaggat tccggatcgc cggcattcca ccaagaattc ctcaacgacg ctggggatat
     2161 cctcgggttg aaacggatga agatcaagaa catctccaag gggaagaaca accggttcat
     2221 caaagggagg atgagtctca gacatatttc aacccgggag ctttatgcaa gttaccaatc
     2281 gctgcaggtg ttcacagtga tccgaacctt ttgtatgccc gttccacttg cttcattgtt
     2341 caaactgtag gtgccgctga tcctggccgc tggcggttcg gtaaattgcc gagcaattct
     2401 aaaattgcct cgtacttaac cggtttagtc agatgggcat caaacccagc ctctttagtg
     2461 cggtgtcggt ccctttgctg tccccacccg gtgagcgcga ttagcaccac ctctttgccc
     2521 caaggctgct cgcgaatttt tcgtgcggca tcgtacccac ttaattcagg catagcgaga
     2581 tcgagtacaa tcacatctgg gtgtgagcgc tcagcggctt gaaacgcttc aaggccatct
     2641 tgtgcgattt gcacctcgta accttcttgg cgcagcaatc caccaagagt ttcagcagcc
     2701 tgcgggaagt cgtcggcgac gaggatgcgg cggatcgggc tctttatgct aggagaatta
     2761 ccatcaacat ccgggctcaa cttctgctga gatggtttcc gtagaaccgg caaattgacg
     2821 acaaactcgc taccttgcct agctcctaag ctgaaggcct gcactgttcc tccgtggagt
     2881 tcaaggattc tctgtaccag cgtcaaacca aggccgaggc cgccctcggc gcgggtgaac
     2941 gatcggtcgg cttgatagaa caggtcgaac aaacgtggca ggtcttcggg agcgatgccg
     3001 atcccggtgt ccttaacctg taggaccgca tgatcgttct gcagtccaag agctagccag
     3061 atttggccag ggtcaggcgt gtatttggcg gcattattga tcaagttcat cactacttga
     3121 gttagccgta cccggtccgc atccacgtaa acgggcgatc caggcagtgt aatcgtgatt
     3181 tcgtggcctc gctcttcgat caacggacgg cttgattcta ccgccgtttt gacaatatca
     3241 catagctcta cgtgttcttt atggaggccg agcttttctc gcgtgatacg gcttatatcg
     3301 aaaagatcgt cggtgagccg tgtcagatat gctacttgct tctcgatgac atccctaccc
     3361 cagcgctgtt gggcttcgga aaagccggaa gcctgagata agctaacaac gtttcgcacc
     3421 agtgctagcg gatttcttaa ttcgtgcgct aaggttgcta agaactcgtc cttgcgccga
     3481 tcggcatcgc gcaacgcttc ttcgctttga ctcaaggcgc tgttaaggcg cagcctttct
     3541 actgccagcc aggtgcgctc ggccgcggca gcgatgagtg agatctcccg ctcgctccaa
     3601 ccacgcggtt cagttgtaga aagaaacaac gcggacacca gtctgccttc gcgcaatagc
     3661 ggggttgccg cgaaagctcc gatgccgatg cgctgatagg attcgctgta aatggcggtt
     3721 cggccatctc gcgacgtgtc aaaaactacg atattgtgac cagcctgaca gtcgctgacg
     3781 atcttcgggc caagagcgct cagccgatag gttccaacca aagaaggcag attgagatga
     3841 taatcgcgat gaatagtgaa ccgacctctt tcaggatcgg cctcgatgaa actgcagcgc
     3901 tccgcttgta aatattcgcc aagtgcgacc gcaacagccc aagtcagttc atccgaatct
     3961 gacgccaggc gaatacaatc gcttaggtcg agcaggaaaa gcgtgtccgc ctccgcctgt
     4021 ttgcgctcag tgatatcggt attcataccg acccagccgc gcgctgaccc gtcagccgac
     4081 aatagcggaa ccgctcgaac taaagtccat cgccattgtc catcatggcg gcgaaggcgg
     4141 tactgagctt ctagaggcgt ttttcgcatg attgcatctt cccattgcat ttggactctc
     4201 ttatgatcat tgggatgata cgcattaagc catccggtgc cgcgccgttg ctcgaaggtc
     4261 tgcccagtaa atgctcgcca agacggtgag tcctccacga tatttcctgc gccatcggct
     4321 gtccaaacga tttgtgatga cgcttctacc agcgcacgaa accgctcctc gcttcggcgc
     4381 atcgcatctt gcgcgcgcct gtgacggaga cccaaaccgg cgacaatcca gatcagggtc
     4441 accgtgagcg cgtcattgaa aatcgggatc catccgggaa ttgctggtga gcctggtgtt
     4501 agaaaatagt ccaggatgat gagaacggta ctaatcaggg caacaagaat cttctcgcga
     4561 gctcctggca acgccaggac taatagcaga gcggcaacat aactggtcgg aatcgcaaca
     4621 ccgaatggcg aatataggtc ggctagaaaa acgccaaata gaagcagagt tatggacagc
     4681 caatatatgg cagaaccaga gctgccgaag agcagtggtg ctggcgcggg tggcccgaat
     4741 accggagaaa gaggttttgt gaagttttcg tccataagcc tgcagccgct agttgttcac
     4801 tcagacaagg ttagcagtgt agcaaagacg tcctcgacgc aaaagcagaa tacggcgaca
     4861 tttctaggcc cttcggcggt acaaaaaaat gtgtcagaga gcgtaatcac ggcagattgt
     4921 gcacattgcc aagactgtgt gatggcgaat gcgatccttc ctgtgcattg gtaacccgat
     4981 tcccgcatgc ttgacaagca actcgcgtgc gacgatacat gtcagaagca ctttcaggag
     5041 agattgcatg gcgagccgaa aatattatcg tgacttccgc gatcatttaa aagaacttga
     5101 agcgcgagac aaactgattc gaatccatcg acggatcgat aaagatactg aattgatgcc
     5161 actggttcgc tggcagtttc gcgggttaga cgaagagcag cgcaaagctt ttctgtttga
     5221 aaacgtggtt gatgcgaaag gcaagcagta ttcgatgccg gtgacggtag ggacgttggc
     5281 cgcgacgacc gaaatctatg caaccggttt gatgtgccgc cccgaggaaa tacacgagcg
     5341 ctggaacaag gcccagttga gtcccatcga gccggtgaaa gtcggtgctg cgccggttca
     5401 ggaaattgtc tggacgggca aggagctgct taacggtcac ggactggata tgctccccgt
     5461 gcccatctcc acccccggct ttgataatgc cccctatttg acttcagcca actggatcac
     5521 caaagacatc gaaagcggag tttacaatat cggcaactat cgtagtcaaa tcaaatcacc
     5581 ggatcgcgcc ggcggattat tcacctcgca acacatgggg caacactggc gcaagtgtaa
     5641 ggccaagggt gtgccgttgc aggccgccat cgccatcggc gttatcccta gtatcgccta
     5701 tgccgcgacg gc
//
LOCUS       DASUTG010000692         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_107512, whole genome shotgun sequence.
ACCESSION   DASUTG010000692 DASUTG010000000
VERSION     DASUTG010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107512"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /gene="aceE"
                     /locus_tag="VF089_16020"
     CDS             <1..2083
                     /gene="aceE"
                     /locus_tag="VF089_16020"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX6175515.1"
                     /translation="GLAPIMSIYQARFNRYLKSRGLLSGEEPRVWCFPGDGEMDEPEA
                     SGALTVAARENLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWNVIKVIWGS
                     NWDPLLEADTDGLLLKRMEEAVDGDYQKYSVEPGSYTRKHFFGKYPQLLERVNHLTDD
                     QIRKLLRGGHDPAKVYTAYKAAVEYKGAPTVILAKTVKGYGLGEAGEGRNITHQQKKL
                     NERELREFRTRFDIPLPDEEVVETPFYRPPLDSPETQYLLERRKQLRGFVPERKVRAA
                     ALKVPEAVDYGEFLKGSAEHAMSTTMATVRILTKLLRHEEIGRRIVPIIPDEARTFGM
                     DSLFRQIGIYSSKGQLYEPVDRETLLYYNEAKDGQILEEGITEAGAMSSFIAAGTSYA
                     NLGTNMIPFYFYYSMFGFQRIGDLIWAAADSKSKGFLLGATAGRTTLNGEGLQHQDGH
                     SHLLASTVPTLLTYDAAFAYEIAVIIRDGLKRMYADGEEYFYYLTLYNESYPMPGMPE
                     GAEDGIIKGLYRFKSAPKGKAHKAHLFGSGPLLREALRAQEILAEQFDVSADVWSATS
                     YKLLRGDALRTKRWNMLHPSVAPKKSYLESLLQNEEGVFVAVSDYMKMVPDQIAPWIP
                     GGLTSLGTDGFGRSDTRPNLRRFFEVDAELTTVATLYALYQKGFLPAQTVESALKKLG
                     IDPEKSCPFYL"
BASE COUNT          577 a          594 c          630 g          481 t
ORIGIN      
        1 ggggctggcg ccgatcatgt caatttatca ggcgcgcttt aatcggtact taaaaagtcg
       61 aggcttactg agcggcgaag agccacgagt ttggtgtttc cctggggatg gcgagatgga
      121 cgaaccggaa gcctctggag cactcacagt ggccgctcgc gagaatctgg ataacttgat
      181 ttgggttgtg aactgcaatc tgcaacggct ggacggtcct gtgcgcggca atgggaaaat
      241 cattcaagag ctcgaatcgc tttttcgcgg cgcgggctgg aacgtgatca aagtcatttg
      301 gggatcaaac tgggacccgc tgcttgaggc ggataccgac ggattgctgc tcaaaagaat
      361 ggaggaggcg gtcgacggag attatcaaaa atattcggtt gagcctggca gctatacgcg
      421 taaacacttc ttcgggaagt atccccagct tctggagaga gtcaaccacc tgaccgacga
      481 ccagatacgc aaattgctgc gcggcggtca tgatccggca aaagtttaca cggcctataa
      541 agcagcggtc gaatacaaag gcgcgccgac tgttatcctc gccaaaaccg ttaagggcta
      601 cggtttgggg gaggctggcg aaggtcgtaa catcactcac cagcaaaaga agctcaacga
      661 gcgggagctg cgcgagtttc gcacccgctt cgatattcca ctaccggatg aggaagtggt
      721 agaaacccct ttctatcgtc cacctctgga tagccctgaa acgcagtacc tactcgaacg
      781 aagaaaacaa ttgcgcggtt ttgtcccgga acgaaaagtc agagccgcag cacttaaggt
      841 gcccgaggcg gtcgattacg gcgagttcct caagggttca gcagaacacg cgatgtcgac
      901 cacaatggcg acagtaagaa tcctcacaaa actcctacgc catgaggaga tcggccggcg
      961 gattgtgccg atcattccgg atgaagcgag aaccttcggc atggactctc tctttcgcca
     1021 gatcggcatt tactcgtcga aaggtcaact ttacgaacca gtcgatcgag aaaccctgct
     1081 ctattataac gaagccaaag acggtcagat ccttgaagaa ggcatcaccg aagcgggggc
     1141 gatgtcctcg tttattgccg ccggaacatc ctacgcgaac ctagggacaa acatgatccc
     1201 tttctacttc tattattcga tgttcggttt tcaacggatc ggcgacctaa tctgggcggc
     1261 agcggacagc aaaagcaagg gttttctctt aggcgccacc gccggccgca cgacactaaa
     1321 tggcgagggc ctgcaacatc aagacggtca cagccatctc ttggccagca ctgtgccgac
     1381 actactcacg tacgatgcgg cgtttgccta tgaaatcgcg gtgatcattc gcgacggcct
     1441 aaagcgcatg tacgccgacg gcgaagaata cttctactac ctcactctgt acaatgagag
     1501 ttatccgatg cccgggatgc ctgaaggggc ggaagacgga attatcaagg ggctttaccg
     1561 tttcaaatca gctcctaaag gaaaagcgca caaggcgcat ctattcggca gcggcccgct
     1621 acttcgcgaa gctctgcgtg ctcaggaaat tctcgctgaa caatttgacg tttcagcaga
     1681 cgtatggagc gctacgagtt acaagctact ccgcggcgat gcgcttcgca ccaagcgatg
     1741 gaacatgctg catccaagcg tagcgcccaa gaagtcgtat ctggagtcgc tgctacaaaa
     1801 tgaagagggt gtttttgtcg cagtatcaga ttacatgaaa atggtaccgg atcagatagc
     1861 gccttggata ccgggggggc tgacgagtct aggcacggat ggttttggac gcagcgatac
     1921 gaggcccaat ttgaggcggt tcttcgaagt cgacgctgaa cttaccacgg tggctactct
     1981 ttacgcgctc taccaaaagg gctttttgcc ggcccagacg gttgaatccg ccctgaagaa
     2041 gctcggaatc gatcctgaaa aatcgtgtcc gttttacctc taaccggcaa cctacaaagc
     2101 tgatatggac gttaaactcc cgcaactcgc tgagggtgtg gaatccggaa cagtagttaa
     2161 cattcttgtc tccgaaggcc aggagattaa gaaggaccag gctttcatgg agttggagac
     2221 tcagaaagcg gtcggctcga tcccctcccc cagcagcggc accgttacta aggttcacgt
     2281 ca
//
LOCUS       DASUTG010000693         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_107698, whole genome shotgun sequence.
ACCESSION   DASUTG010000693 DASUTG010000000
VERSION     DASUTG010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107698"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VF089_16025"
     CDS             <1..1551
                     /locus_tag="VF089_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6175516.1"
                     /translation="ENEVRALTAEADPHRKVYWSFGSSLSQVAGEYARANTAILNSYL
                     GPTVERYLKGLEAKLSSGGLKGPLLIMQGNGGVAHREHVSAIANLQSGPAGGMIASAY
                     IAGLLGHKNVITTDMGGTSFDVGLITEGYWRYAQEPIVERFRMLQPIIDIESIGAGGG
                     TIAQVEPETGRLLVGPRSAGASPGPVCYDAGGNQVTVTDADLALGIIDPNYFLGGRKS
                     LNKDMALKLIEEQIARPLKLKVHEAAAGVHEIVNAKMSDLIRRQVVRTGYLPEEFVIY
                     AFGGAGAVHASGFAAELGIKKIYIFATSPVFSAFGAAAADVIRTRVMSCQYILPADSV
                     VINQRLDGIEKEMSADMNREGFKPGRVEFRRFFTIRYRRQSAGVEMPVSWDRFNPKRM
                     SEMQTAFEKKYEELYGVGSGYAKAGIEISEIRVDAVGKVAKPKLTAKRKSNRDARSAR
                     KGKREIFFTRPERKFIDTPVYDYERLGFGAIVRGPAVIELPFTTALVPPEHRVNIDPY
                     LNLVMEVP"
     gene            1571..2584
                     /locus_tag="VF089_16030"
     CDS             1571..2584
                     /locus_tag="VF089_16030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175517.1"
                     /translation="MEQGQKEDRQMMQRALFVFVHACAFVMVLCSASVAEPLRIAVGA
                     ASIATLPTWVAQDGGYFMREGVPVEQIYIRGGPQTFSALIGGDVHIVQVYSAPVLSAR
                     FTGADAVIIAGLVNQPVFSIVSTAGINKTEDLRGKRIGITTFGSATDLALRLALKKWG
                     LKADTEVNLLQMRGVPEILGALQAKAIDAGILSPPTNMVAIKAGFKELSYLPQAGISF
                     QHTTLATTRRYIERNRATLVKVLRAYVVAIERIKADKAFTMKTISKHMRTTDSDVLEY
                     SYNAAAPLFRVPPYPTLEGIQSTLDFLAEKEPKAKQAHPKDFVDNSLLDELGKTSADI
                     PKK"
BASE COUNT          619 a          749 c          769 g          570 t
ORIGIN      
        1 gagaacgaag ttcgtgcttt gaccgccgaa gcggacccgc accgcaaggt ctattggtct
       61 ttcggtagtt cgttgtccca ggtagctggc gagtatgcgc gcgcaaatac ggcgattttg
      121 aatagctatc tcggcccaac cgtcgagcgc tacttgaaag gcttggaagc caaactgtcc
      181 agcggcggac tcaagggacc gctgttgatc atgcaaggta acggcggcgt ggcgcaccgc
      241 gagcacgtca gtgcgattgc caaccttcaa tcgggtccgg ccggcggcat gatcgcatcg
      301 gcttacattg ccggccttct cggccacaag aatgtcatta ccaccgacat gggcggcacc
      361 agtttcgatg tcggcctcat caccgaaggc tactggcgct atgcgcaaga gccgatcgtc
      421 gaacgatttc gcatgctgca gccgatcatc gatattgaat cgatcggcgc cggtggcggc
      481 accatcgccc aggtagaacc cgaaacgggg cgcttgttgg tcggcccgcg cagcgccggc
      541 gccagtccgg gtccggtctg ctacgatgcc ggtggcaatc aagtgaccgt taccgacgcc
      601 gatctagccc tcggtatcat cgatcccaat tattttctcg gcggtagaaa gtctttgaat
      661 aaagacatgg cgctcaagct gatcgaggaa cagattgcgc gtccattgaa gttaaaggtt
      721 cacgaagccg ctgccggagt ccacgagatt gtcaatgcca aaatgtcgga tctaattcgg
      781 cgccaagtgg tccggaccgg ctatcttccg gaggaattcg tcatttatgc ttttggcggc
      841 gcgggcgcag tgcacgcttc aggattcgcc gctgagttgg gcatcaagaa gatttacatt
      901 tttgccacct ctccggtttt ttccgctttc ggggcagccg ctgccgatgt catccgcacg
      961 cgcgtgatga gctgccagta tattttaccg gcggattcgg tcgtcataaa ccagcgactt
     1021 gacggcatcg aaaaagagat gagcgccgac atgaaccgtg aagggttcaa gccgggccgg
     1081 gtggaattcc ggcgtttttt cacgattcgc taccgccggc aaagcgccgg ggttgaaatg
     1141 ccggtcagtt gggatcggtt caatcctaag cgcatgagcg aaatgcagac cgcattcgaa
     1201 aaaaaatatg aggagctgta cggcgtcggc tcgggttatg ccaaggcggg catcgaaatc
     1261 agtgaaattc gcgtcgatgc agtgggcaag gtggccaagc cgaaactgac ggcaaagcgg
     1321 aagagcaacc gcgatgcccg ctcggcgcgc aaaggcaaac gcgaaatatt ttttacccga
     1381 cccgaacgta aattcatcga tactccggtt tacgattacg agcgacttgg ctttggcgcc
     1441 atcgtccgtg gccccgccgt tatcgagctg ccgtttacta ctgcattggt gccaccggag
     1501 cacagggtga acattgaccc gtacttgaat ctggtgatgg aagtgccctg agacgccgag
     1561 cagttgaacg atggagcaag ggcaaaagga ggaccgtcag atgatgcagc gagcgctctt
     1621 cgtttttgtc catgcctgcg cctttgtgat ggtgctctgc tcggcctctg tcgccgagcc
     1681 gctacgtatt gcggtgggcg cggcaagcat cgcgacattg cccacctggg tggcccagga
     1741 cggcggttat tttatgcgcg agggcgtgcc cgttgagcag atttacattc gcggcgggcc
     1801 gcagactttt tccgcgctga tcggcggtga cgtgcatatc gttcaggtct atagcgcacc
     1861 ggtactctcg gcgcgcttta ccggcgccga tgctgttatc atcgccgggc tggtgaacca
     1921 accggttttc tccatcgtta gcactgcggg tatcaacaaa acggaagatc ttcgcggcaa
     1981 gcgcatcggc atcaccactt tcggctcggc tacggaccta gccctgcggc tagcgctcaa
     2041 gaagtgggga ctcaaggccg acaccgaagt caatttacta cagatgcgtg gtgtgccaga
     2101 aatcttgggg gcgcttcagg caaaagccat tgatgcgggt attctttctc cacccaccaa
     2161 catggtggcg attaaggcgg ggtttaaaga attatcatac ctgccacagg ccggaatttc
     2221 gtttcaacac accacactcg ctaccacgcg cagatatatc gaacggaacc gtgccacgct
     2281 ggttaaggta ttacgagcct acgtcgtcgc tatcgaacgc atcaaagccg acaaggcttt
     2341 caccatgaaa acaatctcga aacatatgcg gaccaccgac agcgacgtgt tggagtatag
     2401 ctacaacgcc gcggcgcctc tgttcagggt gccgccttat ccgacactgg aaggcattca
     2461 atcgacattg gactttctgg cggaaaaaga accaaaagcg aagcaagctc accccaaaga
     2521 ctttgtcgat aattccctcc tcgacgaatt gggcaaaacc agcgctgaca ttccgaagaa
     2581 atgaactcag gagccaacaa tgaaaaaaca aaaacagacc atcgacccga ttcgttatga
     2641 aatgtttctc catcgcctct gggccatcgg tgaagaagga cgcatgaccc tgcaacgagt
     2701 gaccgcc
//
LOCUS       DASUTG010000694         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1291581, whole genome shotgun sequence.
ACCESSION   DASUTG010000694 DASUTG010000000
VERSION     DASUTG010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291581"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF089_16035"
     CDS             <1..819
                     /locus_tag="VF089_16035"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6175518.1"
                     /translation="PIKLPNDAKIAVSFFVAFEAFEKYSQYREGGDKPDYASLAYGEY
                     GGKAGIWRIMDVLARNQVKGTIDTNGRIAEDFPDALKELHAGGHEIVGHGWVNDTALL
                     SLEPTREKELIKRTLDSLASVTGKRPVGWVSPGHRINEFTFAHFVDEGIIWTGDLPGP
                     DIPQHRRVNGKPLVIMPRLDYANDLSLIFRAKNPARVYFESFKSAFDLLYAEGRAGSP
                     KLIDALVHAHIGGRPNIIGVFEECIRYAKGFKDVWFCTKGEIATWYLDNYVSKQ"
     gene            827..2008
                     /locus_tag="VF089_16040"
     CDS             827..2008
                     /locus_tag="VF089_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6175519.1"
                     /translation="MSIKSAAVEKFLRQLDLTDLLIKFNWDEWLTIEPPYSDVRISTQ
                     DVVARYNRNGYDWDMHGTLFTPEKEFDSNRAFVFFHGGAGSEKIMDLTPDGRPGLARV
                     VTSQGFKVLALTYPGHYPKGGVWKQPIPERMPVYLMDRELPKEEIFDRNLKCTFNVIL
                     QGAGLLTDEHFAGREIIGFGHSTGGPMAAHLTRFSKKTKVIGLVGFGSGGPDGWRLEW
                     RDKTGAEAYREIPIEHISRRSPDSFKNSGYVDPEDLTPWGGADDYIRLISPLRSQMKT
                     SLCDNQHNAALGILEEYPKRTGLPREEYFDHLQEPDPEWLKKIRVLLTVGENDKGHWV
                     KGERLEDKREMFMGAKYAASTRGAHVVLIPRYGHVGYSELYNEKIAYLWLWALKAGYF
                     D"
     gene            2034..3101
                     /locus_tag="VF089_16045"
     CDS             2034..3101
                     /locus_tag="VF089_16045"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175520.1"
                     /translation="MLARLRLAVVLLIVAHAPVSQGAQSGPMSITELALYQGSDRENI
                     LIEGAKKEGQLTFYTSNTWVAGPVSQEFEKKYPFIKANVWRSDSKALLKRLTEEHSAG
                     KYLADVVETSPEYVNLLVRASLLQDHFSPELSAYDDEAKVKGKSGVLAWTNREIYISL
                     GYNTNLVPSAEAPKTLKDYLDPKWKGKMAIAGTTTGTQWIGAVWDAMGREFLEKLSAQ
                     EINVQNMSGAALSGLVASGEVPLSPTIFNSNIFTHKQKSAPVEWRPIDPVIAGVGSSG
                     LIVNAPHPHAALLFLDYLHSKAGQQVAMKGGLSSARNDIGSLEQKFKKIYMESKYTPE
                     EMEKKFGEWEGLMRKLFIRKR"
     gene            3258..>3459
                     /locus_tag="VF089_16050"
     CDS             3258..>3459
                     /locus_tag="VF089_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6175521.1"
                     /translation="MGSTILITGANGFIGSHFTQLMESHGNRVVPVDVLPRSADLSLL
                     AIQTPTRIMNVTEEGPFRELCRL"
BASE COUNT          840 a          915 c          958 g          746 t
ORIGIN      
        1 ccaatcaaat tgcccaatga tgcaaaaatt gccgtcagtt tcttcgtcgc cttcgaggcg
       61 tttgaaaagt attcgcagta tcgtgaaggc ggtgacaaac ctgattatgc atctctcgcc
      121 tacggcgaat acggcggcaa agccggcatc tggcggatca tggatgtctt ggcgcgtaac
      181 caggtcaaag ggacgatcga taccaacggc cggattgccg aagactttcc cgatgccttg
      241 aaagagcttc acgccggcgg tcatgagatc gtcggccacg gctgggtcaa tgacactgct
      301 cttttatcgc tagagccgac cagagaaaaa gagctgatca agcggacgct cgacagtctt
      361 gcctcagtca cgggtaagcg acctgtgggc tgggtcagcc ccggccatcg catcaacgaa
      421 tttaccttcg cgcatttcgt tgatgaggga ataatctgga ccggcgactt accgggacct
      481 gacattcccc agcatcggag ggtcaatggc aaaccattag tgatcatgcc gcggctcgat
      541 tacgccaacg atctttcgct catctttcgc gccaagaatc ccgcgcgggt ctattttgaa
      601 agcttcaaga gcgcttttga tctgttgtat gcagagggcc gggcaggctc gcccaagctt
      661 atcgacgcgc tcgtgcacgc ccatatcggc ggcagaccca atatcatagg cgtatttgag
      721 gagtgcatcc gctacgccaa aggcttcaag gacgtgtggt tctgtaccaa gggtgagata
      781 gcgacgtggt acctggacaa ctacgtaagt aagcaatgag gacgacatgt cgataaaatc
      841 tgcagcggta gagaaatttc tgaggcaatt ggatttgacg gatcttttaa tcaaattcaa
      901 ttgggatgaa tggcttacca tcgagccgcc ttactccgat gtgagaatat cgactcaaga
      961 cgttgtcgcg cgctacaacc gcaatggtta tgactgggat atgcatggga cgctgttcac
     1021 tccggaaaaa gaattcgaca gcaacagagc ctttgtcttt tttcatggcg gcgcaggcag
     1081 cgaaaaaatt atggatctta cgccagatgg aagacccggc ctggcgcgcg tcgtcacatc
     1141 ccaaggcttt aaagtgctgg ctctcacata cccgggccat taccctaagg gcggtgtctg
     1201 gaaacagcca attcccgagc gaatgccggt ctaccttatg gaccgggaac tgccaaagga
     1261 agaaattttc gatcgcaatt taaaatgcac tttcaacgtg atccttcagg gtgcagggct
     1321 gctcaccgac gagcatttcg ccggtcgtga gatcatcggc ttcggccact cgaccggcgg
     1381 ccccatggcc gctcatctca cacgattttc caaaaagacc aaagtcattg gcctcgtcgg
     1441 tttcggcagt ggcggccccg atggttggcg tctcgagtgg cgcgacaaaa ccggcgcgga
     1501 agcgtaccgg gaaattccca ttgaacacat ttcccgccga tcgccggact cttttaaaaa
     1561 ctccggctac gtagatccag aagatctcac gccgtggggc ggcgccgatg actacatccg
     1621 cctgatcagt cctctgcggt cgcagatgaa aaccagtctg tgcgataacc aacacaatgc
     1681 agccctcggc attctcgaag aatatcccaa gcgcaccggc ctgccgcgcg aggagtattt
     1741 tgatcatttg caagagcccg atccagagtg gctgaaaaag attcgggtgc tgctaaccgt
     1801 cggtgaaaac gataagggcc attgggtcaa aggggagcgg ttggaagaca agcgcgaaat
     1861 gttcatgggc gccaagtacg cggcaagcac gcgcggagcg cacgtggttc tcatcccgcg
     1921 ttatgggcac gtgggttact ccgagcttta caatgaaaaa atcgcttatc tctggctgtg
     1981 ggcattaaaa gcgggctatt tcgattaaac caagctgctc ctgaggagga tcgatgctgg
     2041 cgagattacg actagcagtc gttttgttga tcgtggcgca tgcgccggtg tcgcagggcg
     2101 cacaaagcgg gcccatgtcg atcactgagt tggcgctgta tcaagggtct gatcgcgaaa
     2161 acattcttat cgaaggcgcc aagaaagaag gccaactcac tttctacact tcgaatacct
     2221 gggtagccgg gccagtatcg caagagttcg agaaaaaata ccctttcatc aaagcgaatg
     2281 tttggcgcag cgattccaaa gcattgctga aacgcctcac cgaagagcat tctgccggca
     2341 agtacctcgc cgatgtcgtc gaaactagcc ccgaatacgt aaacctattg gttcgagcta
     2401 gcttgttgca ggatcatttc tcgccggagc tcagcgccta tgacgatgag gcaaaagtaa
     2461 agggcaaaag cggcgtactc gcgtggacca atcgtgagat ctatatcagt ttgggctaca
     2521 acaccaacct ggttccgtcg gcggaagcgc caaagacgct caaggattat ctcgatccta
     2581 aatggaaggg caagatggcg attgccggga ccaccaccgg gactcaatgg atcggcgctg
     2641 tctgggacgc catgggtcgg gagtttttgg aaaaactaag cgcccaggaa atcaacgtgc
     2701 agaacatgtc cggcgctgct ctttcgggtc tggtagcttc gggcgaagtg ccgctctcgc
     2761 caacaatttt caattcgaac atttttaccc acaagcagaa gagcgctccg gtcgaatggc
     2821 ggcctatcga tccggttatc gccggcgtag gcagctccgg cctgattgtc aacgcgccgc
     2881 acccgcacgc tgccttactg tttctagact atctccattc caaagcgggc cagcaggtcg
     2941 ccatgaaggg cgggcttagc tcggcgcgca atgacatcgg atcactggag cagaagttca
     3001 agaagattta tatggagagc aagtacactc ctgaggaaat ggagaaaaag ttcggcgagt
     3061 gggaagggct catgcgcaag ctgttcatcc gcaagcgcta ggcaggcttc tgaaaaactg
     3121 tgtttgcaga ctgctcaaaa gactcagagg ccaggcgcga gaatctcgat gagctgaggc
     3181 gtacgcttca gtacgttgaa gcgaatcggg cgagcgcaac catttcagca gcctgcctag
     3241 tgtgagctga gcttcccatg ggctccacga tcctaatcac cggcgccaac ggattcattg
     3301 gttcccactt tacccagctg atggaatccc acggcaatag agttgttcct gtcgatgtat
     3361 tgccgcgctc agcggatctt tcattgctgg cgatccagac tccaactcgc atcatgaatg
     3421 tcaccgagga agggccgttt cgggagctct gccggttgg
//
LOCUS       DASUTG010000695         6082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_108041, whole genome shotgun sequence.
ACCESSION   DASUTG010000695 DASUTG010000000
VERSION     DASUTG010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108041"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VF089_16055"
     CDS             <1..1077
                     /locus_tag="VF089_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX6175522.1"
                     /translation="RTVIAGYHWFTDWGRDTMISLEGLTLVTRRFLEAGWILRNFAKS
                     IRHGLIPNLFPEGSREGLYHTADATLWFFHAIERYVQATGDRWTLRFLLPKLIEIFEA
                     HIRGTLFGIRVDPADGLLRQGEDGYQLTWMDAKVGDWVVTPRRGKAVEIDALWYNALR
                     LLQKWIEEEEDQKTARDIGAWADKAKESFNQKFWCAEEGYLYDVIDGPEGDDAACRPN
                     QLLAISLDHPILDPSRWKPVVRTVQQRLLTPYGLRSLAATHPDYKSRYFGDIRARDAA
                     YHQGTIWAWLIGPFIDAWLKVYPEDRVGAHELLRGFIPNMNEHGIGSISEVFDAEQPW
                     TPHGCIAQAWSVAEVLRCWIKTTP"
     gene            1374..1805
                     /locus_tag="VF089_16060"
     CDS             1374..1805
                     /locus_tag="VF089_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6175523.1"
                     /translation="MQIRDIMTREVEVVPVDAPVREAAAKMKQLDVGAIPVCDGQKLT
                     GVLTDRDIAVRLAAEGRNPSETRVSEIMTRDLYYCFEDQDVEEAATVMEAGQIRRLPI
                     LDHDRQLVGIVSLGDISVRSDEKTAAAEALEGISEPAAPKR"
     gene            2016..2957
                     /locus_tag="VF089_16065"
     CDS             2016..2957
                     /locus_tag="VF089_16065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6175524.1"
                     /translation="MQNEKYQTAAGLIEVARPKKATADKKKTKGPPSTPAGRALWKGS
                     INFGLVNIPVALYPAETSKSLDLDMLDRRDFAPIKYQRINKRTGKEVPWDQIVKGYQY
                     EKGEYVTLTDDELRSANVEATQSVDILDFVAAAEISPVYFEKPYYLEPQKNGRRAYML
                     LREVLVKSNKVAIARVVIRSREHLAAVIPEWNVLILNLLRFQHELRDSKGLDIPEAGS
                     KGRAISAGELQMAERLIEAMSGPWNPAKYRDEYREDLENLIEKKIKSGRPRAVSTDGR
                     APAPKTGGKVVDIMSLLQRSVAQAQKKDDAPRRHKAS"
     gene            3044..5041
                     /gene="ligD"
                     /locus_tag="VF089_16070"
     CDS             3044..5041
                     /gene="ligD"
                     /locus_tag="VF089_16070"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX6175525.1"
                     /translation="MKRTNPNAIARADSEREPALRSTRRKGVKKTPTSPIQRRRNSRL
                     PDFVPPQLATLVDRVPQGDDWLHEVKFDGYRILCRISASDICLITRNRQDWTERLSFL
                     AKAANELPVDHAILDGEVVALNENGTTDFQLLQNSLSTHASDNLAYYVFDLLHLDGND
                     LMPWPLLTRKETLAEILKAKSSTSAIRYSEHWLGQGDRLYDEACRSGLEGVISKRADQ
                     PYRSGRSRDWVKTKCVQNQEFVIGGFTDPAGSRAGLGALLVGVHDDRGRLVYAGKVGT
                     GFTRQSLTELRNRLDRLVSKATPFVNPPRGAEARGVHWVRPELIGAVSFAEWTSDNML
                     RHPSFQGLREDKPPVAITRERTAGTNNFTTTRPDAAVEVAGVKLTHPDRVLYPEQGIT
                     KRELALYVEQIADWILPHVTGRPLTLVRCPEGSKECFYQRHVNDSASEWIRPVPIEEK
                     GKLVNYVAVDNLQGLIALVQMGVLELHTWGSASDCLDRPDRLIFDLDPDPEISWEPLK
                     QAAQALRGYLSELGLSSFVKTTGGKGLHVVVPLVPKIDWDTAKEFSKQVAQQMADREP
                     DRYTATMSKAKRRGKIYIDYLRNAKTATAVAAYSTRARAGTPVSVPLRWDELGADIRG
                     AHFNLRNTPERLSQIGKDPWHEYETARRRAITAALIKKLRR"
     gene            5088..5813
                     /locus_tag="VF089_16075"
     CDS             5088..5813
                     /locus_tag="VF089_16075"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6175526.1"
                     /translation="MKDTVIFLAGAAFGAGMMYALDPDAGRHRRALTRDQLVRARTRT
                     RETATATARDLLNRSYGIMAGARSRLFEGEINDQVLEQRVRSKFGYVVRNPSAIEARV
                     DSGRVILTGAVRADEVDQLIAEVSAVRGVSTVENRLDVHHDAAHVPELQSGDKPKPSP
                     QRLDIFQRRWSPATRLLVGLSGAAIIYAVARPHFLWSALLSLGLAGYASAGADAGRAL
                     LPRRLKGKQVNALYDPMSTGWSS"
     gene            5864..>6082
                     /locus_tag="VF089_16080"
     CDS             5864..>6082
                     /locus_tag="VF089_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017992703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6175527.1"
                     /translation="MTTAADVLVEAIYDWGVDVIFGLPGDGINGIMEALRKRQEQIRF
                     IQARHEESAAFMACAYAKYTGKIGVCLAT"
BASE COUNT         1389 a         1659 c         1781 g         1253 t
ORIGIN      
        1 cgcaccgtta tcgccggcta tcattggttc accgactggg ggcgcgacac catgatcagc
       61 cttgaggggc tgacgctggt cacccggcgt tttctcgaag ccggctggat tctgcgcaac
      121 ttcgccaaga gtattcgaca cggactgatc cctaatctat ttccggaagg gagccgtgag
      181 gggctttacc ataccgccga tgcgacctta tggttcttcc acgcgatcga gcgctacgtt
      241 caagccaccg gcgaccgttg gactcttagg ttccttctcc ccaaactgat tgagatcttc
      301 gaggcgcaca tccgtggaac tctgttcggc atcagagtcg atcccgctga cgggctgtta
      361 cgccagggcg aagacggata ccaacttacg tggatggacg ctaaagttgg cgattgggtg
      421 gtcactccgc gccgcggcaa ggcggtcgaa atcgatgccc tttggtacaa tgctctgcgg
      481 cttctgcaga aatggatcga ggaagaagaa gatcagaaga cggctcggga cataggcgct
      541 tgggccgaca aagctaaaga gtctttcaat caaaaatttt ggtgcgcgga agagggttat
      601 ctttacgacg tcatcgacgg acctgaaggc gacgatgccg cgtgccggcc gaatcaattg
      661 ctggcgatct ctctggatca tcccattctc gaccccagcc ggtggaagcc ggttgtgaga
      721 accgtccaac agcgtcttct cactccttac gggctgcgct cgcttgccgc aactcatccg
      781 gattacaagt ccagatattt cggcgatatc cgcgcacgcg atgccgccta ccatcaaggg
      841 acgatttggg cctggttaat cggcccgttt atcgatgcct ggctcaaagt ctatcctgaa
      901 gatcgcgtcg gcgcgcacga gcttttgcga ggattcatcc cgaacatgaa tgagcatggt
      961 atcggttcga tcagcgaggt gttcgatgcg gaacaaccgt ggacgccaca cggttgtatc
     1021 gcccaggcgt ggagcgttgc tgaggtgctg cgctgctgga ttaagaccac gccgtgatgg
     1081 agcagtcgaa gcgctaagct gaatcaacag ccctcgcctg tccacttgcc aaaacctgag
     1141 tacttcctca cgcagttcat acatctcgat tatcaaacag agtaattcag cttgacggtc
     1201 tcaggtttcc cattcactgc gctgtcccgc gcgcgcaggg ttcatttagc gcgtgtccca
     1261 aatctaaaat atggatagcg gagtttttgt cattcctagc gattatcgca tgaataactc
     1321 tttatttctt ggcatatgct ttgcggcctt aaggtttgaa aaggaggtcc catatgcaga
     1381 tcagagacat tatgacgaga gaggtcgaag tcgtgccggt cgatgctccg gtgcgtgaag
     1441 ctgctgccaa gatgaagcag ctcgacgtcg gagctatccc cgtctgcgac gggcagaagt
     1501 taaccggtgt gctaaccgat cgtgatatcg cggtgcgtct ggctgcggaa gggcgcaacc
     1561 cgtcagagac tcgggtatcc gagattatga cccgcgatct ttattactgc ttcgaagatc
     1621 aggatgtgga agaagcggct acggtgatgg aagccggcca gatccggcgg ctgcctatac
     1681 tcgatcatga tcgacaattg gttgggatag tttctctcgg cgatatctcg gttcgatccg
     1741 atgagaaaac ggcggcggcc gaagctctcg agggaatctc cgagccggca gcgccgaaac
     1801 gatgagcggc tcgccccata ttttgacggc ttcccttgat cgcccgtgaa gccttcgtag
     1861 caatcgttcg acgcaagctc aacatgtcga gaaggaatac ttatgacgag tgaccggccg
     1921 aagcgtgaca aaaagcgagg cgcagggcgg gtccgcacgt cgggtgaacg gacccgcggc
     1981 gagactgtga agataaagcc caaagacgaa cacgcgtgca gaacgaaaaa tatcaaaccg
     2041 cggcgggttt aatcgaagtg gctcgaccaa agaaagcgac cgctgacaaa aagaagacaa
     2101 aaggcccgcc aagtacgcct gccgggcgcg ctctgtggaa aggttcgatc aactttggac
     2161 tggttaacat cccggtggcg ctctatcccg cagaaacatc aaaatcgttg gatttagata
     2221 tgctcgatcg gcgcgacttc gcgccgatta agtaccaacg catcaacaag cggaccggca
     2281 aggaagtacc ctgggatcag atcgttaaag gttaccagta cgagaagggc gaatacgtca
     2341 ctttgaccga cgacgagttg cgcagcgcta atgtcgaggc aactcaatcg gtcgatattc
     2401 tcgacttcgt ggcagccgcc gagatcagcc cggtctattt cgaaaagccg tactacttgg
     2461 aaccgcaaaa aaacggtcgg cgcgcctata tgctgctacg tgaggtgtta gtcaaatcca
     2521 acaaagtcgc catcgccaga gtagttattc gcagccgcga gcatcttgcg gcggtgatcc
     2581 ctgagtggaa cgtgctaatt ctcaacctgc tccgctttca acacgaactg cgtgacagca
     2641 aagggttgga tattccagag gcgggtagca aaggtcgggc aatcagcgcc ggcgaactgc
     2701 agatggccga acggttaatc gaagctatgt cggggccctg gaatccagca aagtatcgcg
     2761 atgagtaccg ggaagatctt gagaatctga ttgaaaagaa aattaaaagc ggccggccgc
     2821 gggcggtctc aaccgacggt agagctcccg cacctaaaac cggaggcaag gtggtcgaca
     2881 ttatgagctt gctccaacgc agcgtggctc aggctcagaa aaaggacgat gctccacggc
     2941 ggcacaaggc gagctgaacg caccgccgga catcactgat gaaaacggaa tcaaggctaa
     3001 atgcggacct cgttggagcg gcacgagttg cgattttgaa ccgatgaaac gcactaaccc
     3061 caatgccatc gcgagagcgg attccgaacg ggaacctgct ttgcgcagca cccgccgcaa
     3121 aggagtaaaa aaaactccca catcgccgat ccagcgacgg cggaactctc ggttgccgga
     3181 tttcgtgcca ccgcagttag cgacattagt ggatcgcgta ccgcagggcg atgattggct
     3241 gcacgaggta aagtttgacg gttaccggat cctgtgccgg attagcgcta gcgatatttg
     3301 cttaattact cgtaaccgac aggactggac cgaacgtttg agtttcttag cgaaggctgc
     3361 gaatgaactg ccggtcgatc acgcgattct cgacggcgaa gtcgtggctc tcaatgaaaa
     3421 cggcaccact gattttcagt tattgcaaaa ttctctgtcc actcatgcca gtgacaatct
     3481 agcctactac gttttcgatt tacttcatct tgacggcaac gatctgatgc cgtggccgtt
     3541 gctaacgcgc aaagagactc tggctgaaat attgaaggcg aaatcgtcta cgtcggcaat
     3601 ccgatatagc gagcattggc ttggccaggg cgatcgcctg tacgacgagg cttgccgctc
     3661 cggcctcgag ggggtgattt ctaaaagagc cgatcaaccg taccggtcag gccgcagtcg
     3721 tgactgggtc aaaaccaaat gtgtccaaaa tcaagagttt gtcattggcg gttttaccga
     3781 tccagccgga tcacgcgcgg gcctcggcgc tcttctcgtc ggagttcacg atgaccgtgg
     3841 ccgtctggtt tatgccggca aggtcggcac cggattcact cgccagagtc tcaccgaact
     3901 gcgcaaccga ctggatcggc tggtttccaa ggcaacgcct ttcgttaatc cgccgagagg
     3961 cgcggaggcg agaggcgtcc actgggttag accggaactt atcggcgccg tgtcgtttgc
     4021 cgaatggacc agcgacaaca tgctgcgcca tccgtcattt caagggttgc gcgaagacaa
     4081 gccgccggtg gcgattacgc gcgagcgcac ggcagggaca aacaatttca ctacgaccag
     4141 gcctgacgca gccgtagagg tcgccggcgt caagttgact catcccgacc gggttcttta
     4201 tcccgagcag ggcatcacta aacgtgagct cgctctttac gtcgagcaaa ttgccgattg
     4261 gattctgccc cacgtgacag gcaggccatt gactcttgtg cgctgtcccg aaggcagcaa
     4321 agagtgcttc taccagagac acgtcaacga ttccgccagc gagtggatcc gtccggtgcc
     4381 gatcgaggaa aaaggcaagc tggtgaatta cgtcgccgtg gataatctcc agggactgat
     4441 cgcgctggtg caaatgggag tgctggagct gcacacctgg ggctccgcca gcgattgtct
     4501 ggatcgaccc gatcgtctga tcttcgatct ggatccggat ccggagatat cttgggaacc
     4561 gctcaagcag gcagcgcaag cattgcgcgg ctatcttagc gagttgggat tatcgagctt
     4621 cgtcaaaacc accggcggca aggggcttca cgtcgtcgtt ccgcttgtcc cgaagatcga
     4681 ctgggacact gccaaagaat tttccaagca ggttgcgcag cagatggcgg atcgggagcc
     4741 ggaccgttat accgcgacta tgtccaaggc caagcgccgg ggcaagattt acatcgatta
     4801 tttacggaac gccaagaccg ctaccgctgt tgccgcctac tcaactcggg cacgcgccgg
     4861 aacgccggtg tcggtgccct tgcgctggga cgagcttggc gccgatatcc gcggcgctca
     4921 tttcaatctg cgcaacacac cagaacgctt gtcacagatc ggtaaagatc cgtggcacga
     4981 atacgaaacg gcacgccgcc gcgcgatcac agcggcgctg atcaaaaaac tgcgacgata
     5041 atacgcgctc ggtcacgctc gcaacaagac aagtgaaagg aggttgtatg aaagacaccg
     5101 taatatttct cgccggcgcc gcatttggag ccggtatgat gtatgccctc gatcccgatg
     5161 ccggacggca tcgccgcgcg ttgacgcgcg atcaactagt cagagctcgt accaggacgc
     5221 gcgaaaccgc aacggctacg gcccgggatc tcctaaatcg cagctacggc atcatggctg
     5281 gagcgcgctc ccgcttattc gaaggggaga ttaatgacca agtgttggaa caaagagttc
     5341 gctcaaagtt tggctacgtt gtgcgcaatc cttcggcgat cgaagcgcgg gtcgactccg
     5401 ggcgtgtgat tttgacgggc gcagtgcgcg ccgatgaagt cgatcaattg attgcggagg
     5461 tctccgcggt gcgaggcgtg agcacggtgg agaatcgatt ggacgtgcat cacgatgcgg
     5521 cccatgtgcc cgaactgcaa agcggcgaca aacccaagcc aagcccgcag cggcttgaca
     5581 tctttcaaag gcgctggtca ccggcgaccc ggctgctggt gggcttgtcc ggcgctgcga
     5641 tcatctatgc cgttgcgcgg ccgcattttc tctggagcgc gttattgtcg ctcggtctgg
     5701 cgggttacgc ctcagccggc gccgatgccg ggcgtgcgct gttgccgcgg cggctcaaag
     5761 gcaagcaggt caatgcgctg tacgatccta tgagcacggg ctggagtagt taaccaaagc
     5821 ggggccaact cttcgtgttt cgaatcagtt gaatggaggc atcatgacca ccgctgccga
     5881 tgtccttgtc gaagccattt acgattgggg agtcgatgtc atttttggcc ttcccggcga
     5941 cgggatcaac ggcatcatgg aggcgttgcg caagcggcag gagcagatcc ggtttattca
     6001 ggcgcggcac gaagagtcgg cggcattcat ggcctgcgct tatgccaaat ataccggcaa
     6061 gatcggcgtc tgcttggcga ct
//
LOCUS       DASUTG010000696         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_255895, whole genome shotgun sequence.
ACCESSION   DASUTG010000696 DASUTG010000000
VERSION     DASUTG010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255895"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1415)
                     /gene="dinB"
                     /locus_tag="VF089_16085"
     CDS             complement(324..1415)
                     /gene="dinB"
                     /locus_tag="VF089_16085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6175528.1"
                     /translation="MRIIVHVDMDAFYAAIEERHNLALRGLPVVVGADPKEGRGRGVV
                     MTANYQARKFGIRSALPISRAWRLAQAARRRGEPETIFVRSNFQLYETVSNRIMQIFE
                     RFSDAVEEASIDEAYLDGSSHESFEQAHASMMTLKREILEQEGLGCSIGIAPNKLIAK
                     IASSRSKPNGLTVITQQEVQKFLDPLPIRVIPGIGPKSEAFLHQQNVRLVRELREIPQ
                     ATLTDWFGSWGLRLFEKAQGIDDSPVSNEWIRKSIGEQETFEHDSRDLALVRERLERM
                     AERVLAKLRTNEFSGFHTLTLTVRFSDFVTRNRSRTIKSGIALDKNGDALRLIQKHVL
                     ELLLPFFDARENPRGKAIRLIGLRLEKLF"
     gene            1524..1988
                     /locus_tag="VF089_16090"
     CDS             1524..1988
                     /locus_tag="VF089_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6175529.1"
                     /translation="MAKESAGIVLYRYRDSTLEIFLVHPGGPFWKNKDSGAWSIPKGE
                     FDKREDPLQAARREFHEETGCSVDGSFIALRPVRQAGGKMVHAWAVEGDFEAESIRSN
                     SFTLEWPPRSGQRKDFLEVDRAGWFSLELAREKILKGQLMLLDELQRILNDA"
     gene            2545..2865
                     /locus_tag="VF089_16095"
     CDS             2545..2865
                     /locus_tag="VF089_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175530.1"
                     /translation="MKLFLVLRQKTAGQCSIVAARSKRDAINKITRLEGLDDAWAIRV
                     EEFMITFKMEENTAFFQPDLERAVMSMNITNQRGSRGRIFRLKGDAVMKKRKVANETS
                     MAIH"
BASE COUNT          770 a          804 c          837 g          723 t
ORIGIN      
        1 cgccctcgcc gtatgcggga tcaccgcctc ccggcgagtt caccgaactt caaaacaatt
       61 caaagcgatt tgtcctgata cgtgaactgc agtcaataag caagttgttt cctataaatt
      121 gaattcggtg catgactctc agacattatg agcttcttac aatggactcc tgcagcaaca
      181 aagaggcacc cgaattttag cagtctgatc cggtcagcag tggtggtcat cgattttggc
      241 tcgggtgagg tgtcgttctg acacccttga gaggttacat cggtgcgtga cctgtctgtg
      301 tctcagagga tccgagcgcc gtatcaaaaa agtttctcta gacgcagccc gatcaggcgg
      361 atggcctttc cgcgcgggtt ttcccgcgca tcgaaaaagg gcaggagcag ctccagcacg
      421 tgcttttgaa tcagtcgtaa tgcatctccg tttttatcga gggcgatacc actcttgatg
      481 gtgcgggagc gattccgggt gacgaaatca gagaaacgca ccgtcaacgt caaagtatga
      541 aaccctgaaa attcgttcgt ccgcagcttg gcaagcaccc gttccgccat tctttcaagg
      601 cgctcgcgaa ccaaagcgag atcccggcta tcgtgctcga aagtctcctg ttcgcctatc
      661 gatttcctta tccattcgtt cgataccgga gaatcatcga tgccctgagc cttttcaaaa
      721 agccgcagcc cccaactgcc gaaccagtcc gtgagcgtcg cctgtggtat ctcccggagt
      781 tctctcacca ggcgaacgtt ctgctggtgc agaaaggctt cgctcttagg accgatgccg
      841 ggaatcacac gaataggaag aggatccaag aacttctgaa cttcctgctg cgttatcacg
      901 gttaagccat tcggcttgct tcggctggaa gcgatcttgg cgatcagctt gttcggcgca
      961 ataccgatcg agcagccgag cccttcttgt tcgagaattt cacgcttaag cgtcatcata
     1021 ctggcgtgag cttgctcaaa gctctcgtgc gacgagccgt caaggtaagc ctcatcgata
     1081 ctcgcttcct caactgcatc tgaaaaccgc tcgaaaatct gcatgatccg gttggaaacc
     1141 gtttcataca gctgaaagtt tgaacgaacg aaaattgttt cgggttcacc tcgcctgcgg
     1201 gcggcctgag caagccgcca agcgcgggaa atcggcagtg cggagcggat gccgaatttc
     1261 ctcgcttgat agttggccgt catgaccacg cctctgccgc gtccttcctt gggatcggcg
     1321 ccgacgacca ccggaagtcc gcgcagtgcc aagttatggc gctcctcgat cgcggcgtag
     1381 aatgcgtcca tatcgacatg aacgatgatg cgcatcagat taccagagca gttcgttata
     1441 gtgcctttta gatctgcgag gattccgata gatcatagac cggacaaatc tgctaacagt
     1501 tgtcctaagc ttgagcgaaa agcatggcca aagaaagcgc cggcatagtg ttatatcgat
     1561 atcgcgactc tacattggag atttttctcg tccatccggg tggtccgttc tggaagaaca
     1621 aggattctgg agcatggtcg attcccaaag gcgagttcga caagcgggag gatcccctgc
     1681 aagcagccag gcgcgagttt cacgaagaaa caggatgttc ggtggatggg agtttcattg
     1741 ctctcaggcc ggtgcggcag gccggcggta agatggtgca cgcatgggcc gtcgaaggcg
     1801 attttgaagc ggaatccata agaagtaatt catttacact agagtggcca ccacgctccg
     1861 gacagcgcaa agatttcttg gaagtcgacc gagccggatg gttcagcctc gaattggcac
     1921 gggaaaaaat tctcaagggt caactcatgc tgctcgacga acttcaacga atattgaacg
     1981 acgcttagcg gttgctggag aagtttaatt tcaattcccg gcccatgaaa tctggggtgc
     2041 gctttgggaa acaagcagac cccacgccat ctcataaccg cagtcgccgc acagcccttg
     2101 ttaggacgaa agatccgccg cgttaaactc tgcaatagaa ccacccagcc attttgcaaa
     2161 gtggatatcg acgagcgagg tatctagcgc tatccgaggc taactttggc ggtcggggcc
     2221 cacaacgaag ctcaaactct gaagtttggc agcaatcact cggaaccaga aactcacggg
     2281 ccaaaagatt tgaagagggc ggaccggttc tagatggtgt gacgcggagg tccattaggt
     2341 gtggcctggg gaggccttga cggagctacg caagtggctg gatagggtaa gctttagagt
     2401 ggacaaaaat gttagccggt gttcaaaact tgacaagatt cctcggcatt ttaggtcgga
     2461 ccgcaagcaa acctgctatt attcgacacc tcagtttggc acttccattg cttttcacgg
     2521 tatgcactca gcggaggtta tcgaatgaag ctcttcctgg tattgcggca aaaaaccgct
     2581 gggcagtgct cgattgtagc ggctcgaagt aagcgcgacg cgatcaacaa aataacacgg
     2641 ctcgaagggc tggatgatgc gtgggcgatt cgagtcgaag aattcatgat cacgttcaaa
     2701 atggaagaga atactgcgtt ctttcaaccc gacttagagc gcgcagttat gagcatgaac
     2761 atcaccaatc agcggggtag tcgcggtagg atcttcaggt tgaaaggcga tgccgtcatg
     2821 aagaagcgga aagttgccaa cgagacctca atggcgattc actagcggct tgcggaaagg
     2881 aaaccctatt tttgcttgcg cgttatcgcg ctcgctccaa tcaacctata agtcttcggt
     2941 cgtcgcttga ttgcgcatta gttcctcagt cgttctaagc atccaacata gtgtacatca
     3001 gaaatgccaa tgattcggcg gcgtggaatt gcaagaggac ggtctgctgt agcaatgttc
     3061 aggtacctaa acattgctac aggagatcgt cctctcgcgg atccaagcct tcaatcaccg
     3121 gcatttaatg aaac
//
LOCUS       DASUTG010000697         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1888797, whole genome shotgun sequence.
ACCESSION   DASUTG010000697 DASUTG010000000
VERSION     DASUTG010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888797"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VF089_16100"
     CDS             complement(<1..525)
                     /locus_tag="VF089_16100"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175531.1"
                     /translation="MFDLMLKGGRVYDGSGMPSFTGDVAVAGGKIAAIGRLSGPTKRT
                     LDVEGLAVAPGFIDPHTHLDAQLLWDPLGTSSCFHGVTSSVVGNCGLSLAPCKRDGRE
                     AVIKSFVRVEAISRTVLEEGVEWKWVSTGEYLDALGLRLGINVAALIGHIAVRHYVMG
                     EDAVEREARPEEVAQ"
     gene            complement(648..1388)
                     /locus_tag="VF089_16105"
     CDS             complement(648..1388)
                     /locus_tag="VF089_16105"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX6175532.1"
                     /translation="METTTFLVRTMSDTVETDIQRVLTITTEILPKYGLHPLPFERLR
                     KIADGPFDLFLIPLIFAEEYKEDANVIFNEKKRLEIRTHALEIARKHSFDTKPPNFVP
                     GIEESLKSTQAAGLRSVLLTTGGRRYQHHAMETFGIGKYFAEIVDRDETYYAKEQGLY
                     YLFREHKLPSLRIVLLSGTASYIRAGNNVDGCKVAGNHLEVVTIALSNGYSYNDEETL
                     MAAKPNMLIHSQDQLLPMLKENGLLPGA"
     gene            1724..2266
                     /locus_tag="VF089_16110"
     CDS             1724..2266
                     /locus_tag="VF089_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6175533.1"
                     /translation="MKYGTVGTGILIAFLLPLVFNGDGSSEDKQANKEAQKIAEKVRR
                     KAESTQKAEGSQDLVKDEVTVKTGEKGQPVVEQVGEASFYGDGFHGKKTASGEKFDQN
                     DLTAAHPTLPLGTNATITNLETGNSVEVNINDRGPYAKGRDIDLSKGAAKELGMTKDG
                     VAPVKIEAEVAPDDKGNEKK"
BASE COUNT          633 a          668 c          623 g          551 t
ORIGIN      
        1 ctgggcgact tcctccggtc tcgcttcacg ctccacggcg tcttccccca tgacgtaatg
       61 tcgcacggca atatgaccga tcagggcggc gacattgatg cctagacgca agccaagcgc
      121 atccaggtat tcgccggtgg atacccactt ccattcgact ccctcttcga gaaccgtacg
      181 gctaatggct tcaacccgaa caaagctttt gatcaccgct tcgcgaccgt cacgcttgca
      241 tggcgccaag ctcagcccgc agtttcccac caccgacgag gttacgccat gaaaacacga
      301 cgaggtgcct agaggatccc agagcaactg cgcatcgaga tgcgtatgcg gatcaatgaa
      361 tccaggcgcc accgccagcc cttcgacatc caacgtacgt ttagtcggtc cactgagacg
      421 gccgattgca gcaatctttc ctcccgccac agcgacatcg ccggtgaagc tcggcatacc
      481 cgaaccgtcg taaactcttc cacccttgag cataagatcg aacatcgtgc ctcctaaggc
      541 gacagccgta aaatagttac aggttcagaa aaaataggat catcatgcag tgcacgccct
      601 tagatcaaaa tctctactcg ctgctcggcg cagagaccaa aactctgcta tgcccccggc
      661 aagagcccgt tctctttcaa catcggcaat agctgatcct gactgtgaat cagcatgttc
      721 ggtttggctg ccatcaaggt ctcttcatcg ttgtaggagt atccattgga gagcgcaatc
      781 gtgacgactt cgagatgatt gcccgcgact ttacatccgt cgacattgtt tccggcgcga
      841 atgtaggatg cagttccact gagcaaaaca attctcaagc tcggcagctt gtgttctctg
      901 aacagatagt acaggccctg ttctttggca tagtaggtct catcgcgatc gacgatttca
      961 gcgaagtatt tgccgatgcc gaaagtctcc atcgcatgat gttggtagcg ccgtccgccc
     1021 gtggtcaata gtacactgcg cagcccggcg gcctgagtgc tcttcaacga ctcctcgatt
     1081 ccgggaacga aattcggcgg tttggtgtcg aagctgtgtt tccgcgcgat ctccagcgca
     1141 tgggtgcgta tctccagacg ctttttttcg ttaaagatca cgttggcgtc ctctttgtac
     1201 tcctcggcga aaatcaaagg gatgagaaac aaatcgaagg ggccgtcggc aatctttcgc
     1261 agccgttcaa aaggcaacgg atgcagtccg tacttgggca atatctccgt ggtaattgtc
     1321 agaacccgtt gaatatcggt ctccaccgta tcggacattg tcctaaccag aaacgtcgtg
     1381 gtttccatgg ggtgcctaca gaccagccga tactagtcga agctatttgg gctcgcaagc
     1441 gactcctcag tcttgatcac cgactcgcga accacggttc acttgaaaca ggaatttcgt
     1501 tcttggaaca gcaatttgcc gtccgcaaat gggacagtgt gaaccaccgc gatagcttca
     1561 aacatgacgt acagaagaat cgaagcatct agaaacgctg ctctttattc ggtcaccctc
     1621 aatgcgttag cgcgtagtct ttcgttggcg cacgctttgc tcagagttta agcagattta
     1681 gtgagcatcg aggttcattg aagattcagg cgaaagcaaa gcaatgaagt atggaaccgt
     1741 tggtactgga attctgatcg cttttctgct cccgctcgtc ttcaatggcg acggctctag
     1801 tgaggacaag caagcgaaca aagaggccca gaagatcgcc gaaaaagtgc ggcggaaggc
     1861 cgagagtacc caaaaagctg agggcagtca ggatttggtg aaggacgaag ttacggtaaa
     1921 aaccggcgaa aaaggtcaac ccgtcgtcga gcaagttggg gaagcctcct tttacggtga
     1981 tggttttcac ggcaagaaaa ctgcttcggg agagaaattt gaccaaaatg atttaactgc
     2041 ggcccacccg acccttcctt tggggaccaa cgcaaccata acaaacttag aaactggcaa
     2101 ctcggtcgaa gttaatatta acgatcgggg tccgtacgct aaaggccgcg atatcgatct
     2161 ttcgaagggt gcagctaagg aattaggtat gactaaagac ggcgtcgccc cggtaaaaat
     2221 cgaggcggag gtggctccag atgataaagg caacgagaag aaataggcgc gcgatttaaa
     2281 ttaagaacaa ctgcctatcc cgcctacaga aatctgatga ccagctaccg gccaaaaatc
     2341 agccgggcga gatcgatcga gtcagaaaac ctttagttta tgtgagaatc cggccgcgaa
     2401 ttaacagtcc gccgatctca tcgcctactc cagcgataat cctaattcac gtgaagagct
     2461 gagcgtaccc gattc
//
LOCUS       DASUTG010000698         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_848719, whole genome shotgun sequence.
ACCESSION   DASUTG010000698 DASUTG010000000
VERSION     DASUTG010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848719"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1013..1318)
                     /locus_tag="VF089_16115"
     CDS             complement(1013..1318)
                     /locus_tag="VF089_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6175534.1"
                     /translation="MVFSYALSRKAARRWTVNLDQHLVWMKQQHDNGSILFSGPTPDR
                     KFGIYVVKAGSKAQAEEIARSDPYTAAGFCGYELYEWDVHQIMGVGPFTAAELRAHR"
     gene            complement(1415..2278)
                     /locus_tag="VF089_16120"
     CDS             complement(1415..2278)
                     /locus_tag="VF089_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6175535.1"
                     /translation="MPAELIGLLAAVSYALFTVYGWFGLNYSSALVATLVSLASRTIM
                     LWLAVVFTGGIPQFSSRALWVFVGLGVLQSATSLLTFVGLQKIGTARSQPLRNTYPLW
                     SSLIAITFMHEQAGPPVLGGTILVVLGVALITWQPDAKAIDYCWWHVLYSLGAGILAG
                     VAFPLRRYGLTITNEPVFFAAIVAVVSLVCSLPYAWISHRNQGIVWHPKSLMHFSASG
                     FFEGLGALLSLMALAVGTVVVVSPIVATTPLWNLLIAYLFLRETEKLNARTALGTIAV
                     VGGTIGITLGR"
     gene            2408..>3203
                     /locus_tag="VF089_16125"
     CDS             2408..>3203
                     /locus_tag="VF089_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6175536.1"
                     /translation="MGRIIIVLLTLLFVDLGTSDLRAQDQIRDLVVKIHAVHHTPDVL
                     RPWSRNTPQQIKGSGVVIDGNRILTNAHVVRYASQIYVQPNQSSQHLPARVDAITPSM
                     DLAILKLDDETFFDKRGSLPFAKELPRVKDNINVYGYPTGGTELSVTQGIVSRIEYTD
                     YYYQAAGLRIQVDAALNFGNSGGPAVSDGKLVGLVFSLIQNAQNIGYLIPVEEIQLFL
                     EDVNDGVYDGKPQTHDLIQTVENAALRQRLGLPKGLSGVMVAQPYRD"
BASE COUNT          806 a          871 c          834 g          692 t
ORIGIN      
        1 gagggcccct atcctgcgcc ttccaacgaa ctcgtcattt caggctcgat ctggcgacgc
       61 cgttatccct ttcgccagca ctggcgcctg cgccgcgctt tacgcgccgg aaagtcctcg
      121 tctcaatgaa caaatgcatg agcgaatctg gcttttcatc accgctcgac gagctaagag
      181 agcctcatga tccgtaatgg ccaatcgtgc tgttcgggta tgctttcgaa tcagcgatgc
      241 tttgatatgc acgtgccata tccgcacaag attgagacgt aaatagaatg gcgagtcgtc
      301 agtgtccgaa tggaaggcag catcggttat ctatcacctt cggtttttac aactccctct
      361 gattgggatc ttatcctccc aaaggattta ccatcgtctc ttcaggaaag cgttcaatct
      421 cgtaccgagg cggaattcga taagccaatc gaggccttcg gcgtcggtct cggtatctga
      481 cggctgaaat gtgcgatgtg gttctcatgg gtggtatcaa cttggaagtc agcgtggtta
      541 cgccgccgag caacagctgg ctgccgaatt gtcgcggcag gttgagttgc cggtgagcac
      601 cgcgatgagc gctaccgtcc aggcgatgaa atatttaaaa atccgtagca cagtgatgct
      661 acagcctata aacaaagtat caacgaggcg gtaaaaaata ctacgaagat ggtggactcg
      721 aggtactgtc tatcaagggt ttagaggttt ccaaacccgt ggatcaggtg aagcttccaa
      781 attatgtatc ctagaaggtc gctcgcgagc tttacgcgca gtatgcaacg accgaggttg
      841 gcgctcactc gcctacgttc aggagctcga agatgatacg caaaaaactg cggttccagc
      901 actacagcat cgctctggtg ggtcatgcac gttctcgcaa tcgaagtccc gatcccgggc
      961 ttcggcaaac ttctgcgcgg ctgagttcag cacccaatta attgctcaat tcctagcgat
     1021 gcgcgcgcag ctccgcggca gtaaatggtc caacgcccat gatctgatgg acgtcccatt
     1081 catagagttc gtagccgcaa aaacccgctg ccgtgtacgg atcgctcctg gcaatctcct
     1141 cggcttgagc tttggatccg gccttaacca catagatgcc gaatttccga tccggcgtag
     1201 ggcctgaaaa aaggatggat ccgttatcgt gctgttgctt catccaaacc aggtgttggt
     1261 cgagattgac tgtccatctt cgcgcggctt tacgggacaa cgcataacta aataccacat
     1321 ggcattctcc tcgactcaat tagcggattg agacactttg aaccaactta tggatacgtc
     1381 ctcgaattta gtcttgtcaa gatagctcga tcccctacct gcccagtgtg ataccgatcg
     1441 tgccgccgac cacggcaatg gtacccagcg cggtccgggc gttgagcttt tccgtctccc
     1501 ggaggaacag ataggcgatc agcagattcc acagcggcgt ggtcgcaact atcggtgaaa
     1561 ccaccaccac ggtacctacc gcgagagcca tcaggctcaa cagcgcgccc aagccctcga
     1621 agaaacccga cgccgagaaa tgcatcaaag attttgggtg ccagacgata ccttgattgc
     1681 ggtgcgatat ccaagcatag ggcagcgagc acaccaacga gacgactgcc acgatggctg
     1741 caaagaacac cggctcgttg gtgatcgtca acccataacg gcggagagga aaagcgacgc
     1801 cggcgagaat tcccgcaccg agagaataaa gaacgtgcca ccagcagtag tcgatcgctt
     1861 tcgcatccgg ctgccaagtg atcagcgcga cccctagaac gacgagaatc gttccgccta
     1921 acacaggcgg gccagcctgt tcatgcatga aagtaatcgc aatcaacgaa ctccacagcg
     1981 gataagtatt gcgcagcggc tggctccgag cagtgccgat cttttgcaat ccgacgaacg
     2041 tcaacagact agtagcgctt tgcagaactc ctaacccgac aaagacccac agcgcccgcg
     2101 acgaaaactg tggaattccg ccagtaaaaa caaccgccag ccagagcatg atggtgcgcg
     2161 aggctagcga aactagggta gccaccaatg cgctggaata gttcagtcca aaccatccgt
     2221 agacggtaaa cagcgcgtaa gagaccgcag cgagaagtcc gataagctca gcaggcatgg
     2281 caaagcgatc tgttcgggtg tactagacga gctgaattga gtcaaaggtg aaggtaaaaa
     2341 gatcgtttaa ctcaatcggc ctttgacgta tactgtgccg aatttcagtc aaatatcagg
     2401 agatgttatg ggccgaatca tcattgtttt gctgacgctg cttttcgttg acctgggaac
     2461 gtcagaccta cgggcgcagg atcaaattcg cgatttggtg gttaagattc acgcggtaca
     2521 tcacaccccc gatgtattgc gtccatggag ccggaataca ccgcaacaga ttaaaggttc
     2581 gggcgtggtc atcgacggta atcgcattct cactaacgct cacgtggttc ggtatgcaag
     2641 ccagatctac gtgcagccca atcaatcatc ccaacatcta ccggcacggg ttgatgcgat
     2701 taccccgtct atggacctgg cgattttaaa gctggacgat gaaacttttt ttgacaagcg
     2761 cggctcgctt cctttcgcca aagagctgcc ccgcgtcaag gacaacatca acgtttacgg
     2821 ctatccgact ggcggcactg agctttcagt aacccaagga atcgtttcac gcatcgaata
     2881 caccgactac tactatcagg cggcaggtct gcggattcag gtcgatgcgg cgctcaattt
     2941 cggcaacagt ggtggccccg ccgtctcgga cggaaaactt gtcggcctgg tgtttagttt
     3001 gattcaaaat gcccagaaca tcggctacct catcccggtg gaggagattc aactttttct
     3061 cgaagacgtg aacgacggtg tatacgacgg caaaccacaa acgcacgatc tgatacaaac
     3121 agtggaaaat gcagcgctcc ggcagagact cgggttacct aaagggctaa gcggggtcat
     3181 ggtagcgcaa ccctatcggg atg
//
LOCUS       DASUTG010000699         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1889098, whole genome shotgun sequence.
ACCESSION   DASUTG010000699 DASUTG010000000
VERSION     DASUTG010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889098"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF089_16130"
     CDS             complement(<1..255)
                     /locus_tag="VF089_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175537.1"
                     /translation="MVIDFQAHLFPQEYINELKRRDGAVILEEPDPYSGMTYFYDKKL
                     KSRINTAIFQGRDIDRRLEHMDRFGIDIHVLTIPAPGADRF"
     gene            complement(284..1312)
                     /locus_tag="VF089_16135"
     CDS             complement(284..1312)
                     /locus_tag="VF089_16135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175538.1"
                     /translation="MSVIDADAHVLETERTWDYMLESERFMRPRVVPTPNDATSGGES
                     WLVDGTHIGKARNVGHDTAREAREMEDIRARLAHMDELDIDVQVLYPTIFLRPFTRRP
                     EVELALARSYNRWLIDIWKAAPERLRWVAVLPLLTMDKALEEARFAKENGACGIFMRG
                     LEGDKRISDAYFFPLYEEAGRLDLPICVHSATGSFAVHDYFLDDCGFNKFKLAVVGSF
                     HSLIFNKIPERFPQTKWAFIEVSSQWIPYAIHDYARRFERSGAKVDKSDVLRRSRIWV
                     ACQTDDDLPYVLKYAGDSSLVIGTDYGHNDTSSEILALRKLREDGAVPPQVVEKILDD
                     NAKALYSL"
     gene            complement(1326..1526)
                     /locus_tag="VF089_16140"
     CDS             complement(1326..1526)
                     /locus_tag="VF089_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175539.1"
                     /translation="MEQSFDQFNAALLYCNKCGRAMPVRQRLLLVLPDGELYDYTCQT
                     CHSSVGSKTERAGTQATTKKIR"
     gene            complement(1611..3710)
                     /gene="fusA"
                     /locus_tag="VF089_16145"
     CDS             complement(1611..3710)
                     /gene="fusA"
                     /locus_tag="VF089_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6175540.1"
                     /translation="MAGTELQNLRILGILGQGGSGKTSVAEAMLFAAGATQRLGRVQD
                     GTSVLDHEPEEIKHQVSISTAFHSLSWKKHSLTLVDTPGYAAFLADSINCARAFGAAV
                     FVLNPSVGLRVESERLWATANANQICRLFFVNKMDHEEADAVERIDPLLSRLDAKGVH
                     LQLPIGAGTEFKGVIDLPSMKAYLYEGSGGKFSETEIPADLKEAAENMRRQLMDSVAE
                     ADDGFLEKYLDGGEFSLEELKQTIREATRQRKIFPILYGSATHLIGIPQLLEAVLDYL
                     PAPPEEAELEGTNPITREIERRQHDLGAPFSASVFKTLVDPFAGKLSVMRVISGKLSP
                     DETCYNANKQSKEKIGHMFRLEGKKQEAIKEARAGEIVAAAKLKDVSSGDTLCDERAP
                     IQYPPPVHFSPVISFALEPKSKADEEKVPQGLHRMMEEDPTIDLHRDDETRDFILSGM
                     GQQHIEIIVEKLKRKYSAEVILKAPKVPYKETIRGTASAQGKLKKQSGGRGQYGDTWL
                     KVEPLPHGKGFEFVDQIVGGAIPRNYIPAVEKGIKEAMGSGVVAGFPMVDVRVTLYDG
                     SYHDVDSSDMAFKIAASMGFKNAVEKAKPVILEPIMSMEVTVPDECMGDVIGDLNSRR
                     GKVLGMDTNGHTQVIKSRVPMSEVLKYAPDLRSLTSGRGEFHMEFAHYEELPAHLAER
                     VIKEAKAQNKTEQASQH"
     gene            complement(3732..4526)
                     /locus_tag="VF089_16150"
     CDS             complement(3732..4526)
                     /locus_tag="VF089_16150"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX6175541.1"
                     /translation="MLLAIDIGNTNIVLGLYQGKKLITHWRLLTEAERTSDEYGVIIA
                     HLVAYEGFRCNQISAIVVSCVVPPMLTMIQELGKKFFKIEPLMVGPGIKTGMQILYEN
                     PRDVGADRIVNGIAAYERYRDSCIIVDFGTATTIDLISKKGEYVGGAIAPGLSISLEA
                     LSQRASKLPRIEIVKPREVIGRNTVNSIQAGIFYGYVGLVDGIVKRIQKESGVQAKVV
                     ATGGLAPLVASECSTINDVDEFLTLQGLRIIHERNSLQEVKVRAQT"
     gene            complement(4531..5322)
                     /locus_tag="VF089_16155"
     CDS             complement(4531..5322)
                     /locus_tag="VF089_16155"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6175542.1"
                     /translation="MPLDLNSIRQRLAAHRLGTELHYFAEIGSTNAYARRLAEQGARD
                     GELVIADSQTEGRGRLGRRWVSPPNVNLYLSLVLRPKLPPARAPQITLMAAVALAETL
                     KSFLPVPPSIKWPNDIIVNGKKLAGILTEVNCGTDSVEFVILGIGVNVNYPVDLMPEE
                     IRQRATSVLVECQNKVNREDLLQRLIRHLDRCYGEIEENGFAPLAPRWEAYFGWRGQR
                     VRIELLDQIVFGIAKGIDRDGALVLEDDQGRVQRVIAGDVTLMSQ"
     gene            complement(5353..>5767)
                     /locus_tag="VF089_16160"
     CDS             complement(5353..>5767)
                     /locus_tag="VF089_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX6175543.1"
                     /translation="VRMGGGTNHRIGLYDAALVKDNHIEATGSIAEAVKAVRLHAPFM
                     AKVEVEAGNLTQVQEAIDAGADVIMLDNMSVAQMAEAVRLVNKRAWVEASGGITLNTI
                     RQIAETGVDFISSGALTHSAPVVDFNMKISMNLSR"
BASE COUNT         1256 a         1635 c         1453 g         1423 t
ORIGIN      
        1 gaaccgatcc gcgccgggag ccggaatggt caacacatga atatcgatgc cgaaccggtc
       61 catatgttcg agccggcggt caatatcacg cccttgaaaa atcgcggtgt tgatacgaga
      121 ttttaatttc ttgtcgtaga aataagtcat gccgctgtac ggatcgggct cttcgagaat
      181 gaccgcgccg tcacggcgct taagctcgtt gatgtactct tgcggaaata agtgggcttg
      241 aaagtcaata accataggga atcttctgcc tctgggttag ggattaaagg ctgtagagcg
      301 ccttggcatt atcgtcgagg attttctcaa caacctgcgg cggcaccgca ccatcctctc
      361 tgagtttgcg cagtgcaaga atctccgatg atgtgtcgtt gtgcccgtaa tcggtgccga
      421 tcaccaggct actatcgccc gcgtacttaa gcacgtaggg cagatcgtca tcggtctggc
      481 acgcaaccca gattctgctt cgccgcagca catcggattt gtcgaccttg gcgccgctgc
      541 gctcaaagcg ccgcgcataa tcgtgaatcg cgtaaggtat ccattgtgag ctgacctcga
      601 taaaagccca tttagtctgg gggaatctct ccgggatctt gttaaaaatg agcgaatgaa
      661 atgacccgac caccgcgagc ttgaatttat tgaagccgca gtcatctaga aaatagtcat
      721 gcactgcgaa actgccggtt gcggaatgga cgcagatcgg taaatctaga cgaccggcct
      781 cctcgtagag cgggaagaaa taggcatcgc tgattcgttt atcgccctcc agaccccgca
      841 tgaaaatgcc acatgcgcca ttctctttgg caaagcgggc ctcttcgagc gccttgtcca
      901 tggtcaagag aggcaacacc gcaacccagc gcaaccgctc cggcgctgct ttccatatat
      961 cgataagcca ccggttgtag ctgcgcgcca gtgcgagctc aacctctggc cggcgggtga
     1021 atgggcgaag aaatatcgtc ggatagagca cctgcacgtc gatatcgagc tcatccatgt
     1081 gcgcgagccg cgccctgatg tcctccatct cacgcgcttc tctcgccgtg tcgtgaccga
     1141 cgttgcgcgc cttgccgatg tgagtgccgt cgactagcca ggattcgcct ccagacgtgg
     1201 cgtcattagg tgtcgggacg acccgtggtc tcatgaagcg ttccgactcc aacatgtagt
     1261 cccaagtgcg ttcggtttct aaaacgtgcg cgtccgcatc gatcactgac atttcgatca
     1321 gcctcctatc taatcttttt tgtagtggct tgagtgccgg cgcgttcggt ttttgaccct
     1381 accgagctat ggcacgtctg gcaggtatag tcatagagct ctccatccgg cagcaccagc
     1441 agcaaacgct gccgcactgg catcgccctg ccgcatttgt tgcaatacag caacgccgcg
     1501 ttgaattgat caaatgattg ttccataact ccctaatccc tagccgacta ctactttgag
     1561 catctttcct gaggggaagg actttcctca attacgtcgc aggtcgatct ttagtgctgg
     1621 ctcgcctgtt ccgtcttgtt ctgtgctttg gcttctttga taactctttc agctaaatga
     1681 gctggcaact cctcataatg tgcgaattcc atatgaaact cgccgcggcc gctggtgagt
     1741 gaacgaaggt ccggcgcata ttttagcacc tcggacatcg gcacgcggct tttgatgact
     1801 tgagtgtgtc cattggtatc catgccgagt actttgcccc tgcggctgtt caaatcgcca
     1861 atgacgtcgc ccatgcattc gtccggcacc gtgacctcca tgctcatgat gggttctaag
     1921 atcaccggct tggctttttc gacggcgttt ttaaatccca tggaggcggc aatcttaaat
     1981 gccatatccg atgaatcgac atcatgatag gaaccatcat aaagtgtcac ccgcacatcg
     2041 accatcggaa agcccgctac cacgcctgat cccatcgctt ctttgatgcc tttctccact
     2101 gccggaatat aattgcgtgg aatcgcgcca ccgacgatct gatcgacgaa ttcaaatccc
     2161 ttgccgtgcg gcagcggttc gaccttcagc caagtgtcgc catactgacc gcggccaccg
     2221 gattgcttct ttaattttcc ttgcgcgctc gcagtgcccc ggatggtttc tttgtacggc
     2281 actttgggcg cttttaaaat gacctcggcg ctgtacttcc gcttgagctt ctcgacgatg
     2341 atttcgatgt gctgctgtcc catacccgag aggataaaat cgcgggtctc gtcatcgcgg
     2401 tgaagatcga tcgtgggatc ttcttccatc attcgatgca gcccctgcgg caccttttct
     2461 tcatctgctt tgctttttgg ctccaaggcg aaggaaatga ccggcgagaa atgaaccggc
     2521 ggcggatact gaatcggcgc gcgttcatca cacaacgtat cgccgctgct gacatccttt
     2581 agcttagccg ctgcgacgat ttcgcctgcg cgcgcttctt tgatggcctc ttgctttttc
     2641 ccctccaacc ggaacatgtg accgattttt tctttgctct gtttgttggc gttgtaacat
     2701 gtctcgtccg gcgaaagttt tccggagatt acccgcatca ccgacagttt cccggcaaaa
     2761 ggatcgacca gggttttgaa aacagaggcc gaaaacggcg cgccgagatc gtgttgcctc
     2821 ctctcaatct cgcgcgtaat cgggttggtg ccttccaact ccgcctcttc cgggggcgca
     2881 ggtaagtaat cgagcaccgc ctcgagcagc tggggaattc cgatcaaatg tgtcgcagag
     2941 ccatagagaa tcggaaagat ttttcgttgc cgggtggctt cacgaatcgt ctgcttcaac
     3001 tcttccaatg aaaattcgcc gccgtcgagg tatttttcga gaaatccatc atctgcttcg
     3061 gcgacactat ccatcaactg tcgccgcata ttttcagccg cttccttgag atcagcgggg
     3121 atttcggtct cgctgaactt tccgccgctt ccctcgtaaa gataggcttt catcgaaggc
     3181 agatcgataa cccctttgaa ctcggtcccg gcgccgatgg gtaactgcag atgaacaccc
     3241 ttggcatcta acctggaaag caacggatcg attcgctcaa ccgcatcggc ctcctcgtga
     3301 tccattttat tgacgaagaa aagccggcag atttgattgg cgttagccgt cgcccatagc
     3361 cgttcagatt caacccgcaa tcccaccgaa ggattcagaa caaaaaccgc agcgccaaaa
     3421 gcacgcgcac agttgattga atcggcgaga aaggccgcat agcccggggt gtcgaccaga
     3481 gtcaaagaat gttttttcca tgaaagagag tgaaaagcgg ttgagatgga aacctggtgt
     3541 ttgatttctt cgggctcatg atcaagcacc gatgtgccat cttgaacccg tcccagccgc
     3601 tgagttgcgc cggcggcaaa cagcatcgct tctgctacag aggtttttcc cgaacctcct
     3661 tgaccgagaa tgccgaggat tcgaaggttt tgcagctcag ttccagccat aactgtgggg
     3721 tcctccttct gctatgtttg cgctcttacc ttcacctctt gcagggaatt tctttcatga
     3781 atgatcctaa gtccctgaag cgttagaaac tcgtcaacat cgttgatcgt agaacattcc
     3841 gaagccacca gtggcgccaa tcccccggtc gcgacaacct ttgcctgaac tccgctttcc
     3901 ttctgaatcc gtttaacgat gccgtcgacc agacccacgt agccgtaaaa tatccccgcc
     3961 tgtatcgagt tcaccgtatt acggccgata acctcccgtg gcttgacgat ttctatccgg
     4021 ggcagcttgg acgcgcgttg cgacagcgct tccaaagaga tcgataaacc cggagcgatc
     4081 gcaccgccaa cgtattcccc ctttttggag atcagatcga tggtcgtcgc agtcccaaaa
     4141 tcgacaataa tgcacgagtc cctatatctc tcgtaggcgg ctatgccgtt gacaatccgg
     4201 tcggcgccaa catctcgggg attttcataa agaatttgca taccggtttt tatcccggga
     4261 ccgaccatca atggttcaat tttaaaaaat tttttcccca gctcctgaat cattgtcagc
     4321 atgggcggga ccacacagga aacgacaatg gcgctaattt ggttacaacg aaatccttcg
     4381 taagcgacga ggtgggctat gatgacgccg tattcgtcgg aggtgcgctc ggcttcggtg
     4441 agcagtcgcc agtgggtaat cagctttttc ccctggtaaa gtccaaggac gatattggta
     4501 ttgccgatgt cgatggcgag cagcatgttt ctattggctc attaaagtga cgtccccggc
     4561 aatgactctt tgcactctgc cctggtcgtc ttcgagtact agggcgccat cacgatctat
     4621 gcctttggct atgccgaaca cgatctgatc caaaagctca atacgcaccc gctgtcctct
     4681 ccagccaaag taagcctccc agcgcggcgc cagcggcgca aacccatttt cctcgatttc
     4741 tccataacac cggtccaggt gtcgaatcaa tctctgcagc agatcttctc ggtttacttt
     4801 gttctggcat tcaaccaaaa ccgatgtcgc gcgctggcgg atttcctcgg gcataaggtc
     4861 aacgggatag ttaacgttga caccgatgcc gagaatcaca aattcgacgg agtcggtgcc
     4921 gcagttcacc tcggtcaaaa tgcccgctag ttttttgcca ttcacgatta tgtcatttgg
     4981 ccacttgata gatggaggaa ctggaagaaa ggacttcagg gtctcagcca aggcaacggc
     5041 ggccatcaag gtgatctgcg gcgctcttgc aggcggcagt ttgggtcgca gaacaagaga
     5101 cagatagaga ttcacgttgg gtggcgacac ccaacgcctg ccaaggcgtc cccggccctc
     5161 tgtttgcgaa tctgcaataa ccaactcgcc atctctggcg ccctgttcag cgagccggcg
     5221 agcgtaagca ttggtggagc cgatctcagc gaaatagtga agctcagttc caagccggtg
     5281 cgcggctaac cgttggcgga ttgagttgag atcgagtggc aaatgagcgc tgcttcgatg
     5341 attttcggac gatcaccgac tgaggttcat gcttatcttc atgttgaaat cgactaccgg
     5401 agccgaatgc gttagagcgc ccgaagagat aaaatctacc ccggtctcgg cgatctgacg
     5461 gatggtgtta agcgtgatgc cccccgaagc ttccacccag gcccgtttgt tgaccagacg
     5521 caccgcttcc gccatctggg caaccgacat attgtcaagc atgatcacat cggcgcccgc
     5581 atcgattgct tcctgaacct gcgttaagtt gccggcttct acttcgacct tagccatgaa
     5641 cggtgcatgc agtcttaccg ctttgactgc ttcggcgatc gagccggtcg cttcaatatg
     5701 gttatccttt accagagccg catcatacag gcctatccgg tgattcgtgc cgcctcccat
     5761 tcgaacc
//
LOCUS       DASUTG010000700         4910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_552500, whole genome shotgun sequence.
ACCESSION   DASUTG010000700 DASUTG010000000
VERSION     DASUTG010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552500"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1612)
                     /locus_tag="VF089_16165"
     CDS             complement(125..1612)
                     /locus_tag="VF089_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX6175544.1"
                     /translation="MNQIVDSSGDLLFAFDNTYARELEGFYVPWKAAQVARPRLIKFN
                     HQLAEELGLDADTLDTDEGARIFAGNETPEGALPLAQAYAGHQFGGFVPQLGDGRALL
                     IGEVIDRYGRRRDIQLKGSGPTPFSRAGDGRAALGPVLREYLIGEAMHALGIPTTRAL
                     AAVITGEPVYRERALPGAVLTRVAASHVRVGTFQFFAARGEQEKLARLADYVIDRHYP
                     ALKEDASRYLRLLEQVCDRQAALIASWMHVGFIHGVMNTDNMTISGETIDYGPCAFMD
                     HYDPATVFSSIDTQGRYAYANQPRIAQWNLARFAETLLPLIDEDANRAIARATEAVNG
                     FPGHYERYWVKGMRAKLGLFNVEEADLNLGTEFLTAMEGNNVDYTLAFRYLADAALGR
                     QEPVRGLFADPSAYDRWSGHWRARLSREAMEPLERAQAMRRANPAFIPRNHRVEEALS
                     AAVERDDYAPFETLLKILARPFDDQPEFAALADPPPEGQGHYRTF"
     gene            1858..2751
                     /locus_tag="VF089_16170"
     CDS             1858..2751
                     /locus_tag="VF089_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6175545.1"
                     /translation="MRVILMVILMIASIPVRSDAAQTEDGSALPSTQRVTLKWLGTAG
                     WEIQIDQTKILIDPFLTRRQSVAAEEWKTDEEAVLNVISGADYIFAGHSHADHIADIP
                     FIAKRFGAKVIGSRTTVNLALSAGVEQSHLTSVIGGEKLDFKDFSVQVIKSQHGIVTR
                     DGQKRQPRSAEIARPPSGPILGRHFVEGGSYLYYFTFGKFRVLHQSTGNFIEGNLHGL
                     EPDVAILAQNSNYDWSEALKILRPKTVIVHHYDNWRVPLSSGMTAGVRRRAQRLQRDV
                     NSVDRNIKVIIPEFLQTIGLE"
     gene            complement(2768..3583)
                     /locus_tag="VF089_16175"
     CDS             complement(2768..3583)
                     /locus_tag="VF089_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175546.1"
                     /translation="MAQHIDGPLYYERMGRVGPVIAFIHPNPMDQSCWIFQMAHLSTW
                     YRCIAIDIPGYGRSPKAQSGLTMDDMAKACWEAIDDALPGEKAILVGCSVGSSLAPHM
                     YHLRPEQTAALVLSGTGYNPSKEFTKGRIANYTANGIDYRWAYTFEDLSAAFRTTPLA
                     HFFANLFTERNQHADVETIIHQFKALAQPYPENHHERIACPTIILTGSEDNSHQRAFA
                     LQARIPSCELRTLPGAGHACQIEQPWLFDRFMIEFLAKHGLFPGASIPPRTII"
     gene            complement(3701..4468)
                     /locus_tag="VF089_16180"
     CDS             complement(3701..4468)
                     /locus_tag="VF089_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175547.1"
                     /translation="MDSKAKYFDCHFHIIDKNFPVVPNQGFMPDAFTSENYLERLKAV
                     DLCGGAVVSGSFQAFDQSYLFHALKVLGPCFVGVTQVPQAVSDQELQQLNAAGVRAVR
                     FNVKRGGSEEIHHLESMARRVHELVGWHTELYVDSSELADLFETLVSLPAVSIDHLGL
                     SKAGFPTLLKLAEKGVRVKATGFGRVDFDVRSALTDLYTANPRVLMFGTDLPSTRAPR
                     PYQEEDYTLVLETLGEEKAADVFYNNAIQFYRPKEVR"
     gene            4568..4846
                     /locus_tag="VF089_16185"
     CDS             4568..4846
                     /locus_tag="VF089_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175548.1"
                     /translation="MARFKRHDFLRQVPTRETQQNLKEIVRQIQAHGAMVAIAGIKLG
                     FFTDEYGAIFEETAVEYGAIYIPHVMKGISMTQSSGVIRFTLTAPVTI"
BASE COUNT         1094 a         1359 c         1394 g         1063 t
ORIGIN      
        1 gattcagttg cgagtcgatg attgggcata gctcccaact ggcggcgaaa aacctctcgc
       61 gagatctacc ggttcaatgt ggccgtgaaa actttgcgtc tcccggacgc gtgattcagg
      121 tacctcagaa ggttcggtaa tgaccttgcc cttccggtgg gggatcggcc aaggcggcga
      181 actccggctg atcgtcgaac ggccgcgcca ggatcttcag cagcgtctcg aaaggcgcgt
      241 aatcgtcacg ttccactgca gcactcaatg cttcctcgac tcgatgattg cgtggaatga
      301 atgcggggtt ggcacgtcgc atcgcctgcg cgcgctccaa aggttccatc gcttcgcgcg
      361 agagccgcgc gcgccaatgc ccactccatc gatcgtaggc cgagggatcg gcaaagaggc
      421 ctcggaccgg ttcctgccgg cctaaagccg catcggcgag ataacggaaa gccaacgtgt
      481 agtcgacgtt gttgccttcc atggcagtga gaaactcagt cccaagattg agatcggcct
      541 cctccacatt gaagagcccg agcttagcgc gcatgccttt tacccagtag cgttcgtagt
      601 gtccgggaaa gccattgacc gcctcagtgg cgcgcgcgat ggcgcgattt gcatcctcat
      661 cgatcagggg cagaagcgtt tcggcgaatc gcgccaggtt ccactgcgcg attcttggct
      721 gattggcgta cgcgtagcgg ccctgagtat cgatggagct gaacaccgtg gccggatcgt
      781 aatgatccat gaaagcgcat gggccgtaat cgatcgtctc accggagatg gtcatattgt
      841 cggtgttcat cacgccgtgg atgaagccca catgcatcca gctggcaatc agcgccgctt
      901 gtctgtcgca cacctgttca agcaatctta gatagcggct cgcgtcctcc ttcagcgccg
      961 ggtagtgccg atcgattacg tagtcggcca ggcgagctaa tttctcctgc tcaccgcggg
     1021 cggcgaaaaa ctggaaggtg ccgacacgta cgtggcttgc cgcaacacgg gtcaaaaccg
     1081 cgcccggcag cgcgcgctca cgataaaccg gctcgcctgt gatcactgcg gcaagcgctc
     1141 gcgtcgtcgg gatgccaagt gcgtgcatcg cttcgccgat aagatattca cgcaacaccg
     1201 gcccgagtgc ggcacggccg tcgccggcgc gcgaaaacgg agtgggaccc gagcctttga
     1261 gctgaatgtc gcgacgacgc ccgtagcgat cgatcacctc gcctatcagc agcgcccggc
     1321 catcgccgag ctgaggaaca aaaccgccga actggtggcc ggcgtaggct tgcgccaacg
     1381 gcagcgctcc ctcgggtgtc tcgttgccgg caaagatcct ggcaccttcg tcggtatcga
     1441 gagtatcggc gtccagtccc aattcttcag ccagctgatg gttgaacttt atcagccgag
     1501 gtcgcgccac ttgcgccgct ttccacggga catagaaccc ctcgagctcg cgggcatagg
     1561 tgttgtcgaa ggcgaataac aagtcgccag aactatcaac aatctgattc attatctcgt
     1621 tcccttgctg aggacgttag cgatcctact attattgttc tcgatatcga tagtaatatc
     1681 tagacctgct atcgcttaat ggttcagttt gagttttctt gatcgtacgc cggagaaaga
     1741 ttcctcgcat cagctcgaat catcaaaatg gggagtgtca gccacggtat atttaaacaa
     1801 gttgttaact gacagtgagc gtagctctag cgtggatatc agaccaggag gatcatcatg
     1861 cgggtcatct taatggtcat tctcatgatt gcttcgatcc ccgtccgatc tgacgccgcg
     1921 caaacggaag atggctccgc tttgccgtca acacagcgtg tgaccttgaa gtggctcggt
     1981 actgccggtt gggaaattca gatcgatcag accaaaatcc tgatcgatcc cttcctcacg
     2041 cgccggcagt cggtcgccgc ggaagaatgg aaaaccgacg aagaagcggt gcttaatgtt
     2101 atcagcggcg ccgactatat cttcgccggc cacagtcatg ccgatcatat cgctgacatc
     2161 ccgttcatcg ccaagcgctt cggagccaag gtcatcggtt ccaggaccac ggtgaatctc
     2221 gcgttgagcg ccggcgtcga gcaatcgcat ttaacgagtg tcatcggcgg cgagaaattg
     2281 gacttcaaag acttctcggt gcaggttatc aaaagccagc acggcatagt cacccgggat
     2341 ggtcaaaaac ggcagccaag gtccgcggaa atcgcccgcc cgccctcggg tccgattctc
     2401 ggaagacatt tcgtcgaggg tggaagttat ctctattact tcacgttcgg caaattccgg
     2461 gtgctgcacc aaagcaccgg caatttcatc gaagggaatc tccacggctt agagcccgac
     2521 gtcgcgattc tcgcgcagaa cagcaattac gattggagcg aggctctcaa gattctccgc
     2581 ccgaaaacgg tcatcgtcca tcactacgac aattggcggg tacccttgtc gagcggcatg
     2641 accgcaggcg ttagaagaag agcgcaacgc ttgcagcggg acgtcaactc cgtggatcgc
     2701 aacatcaagg tgattatccc ggagtttttg cagacgatcg ggttggaatg atactgcggc
     2761 ggcgctccta gatgatcgtc ctgggtggaa tcgatgctcc ggggaacagc ccgtgcttgg
     2821 ccaaaaactc gatcataaac cgatcgaaga gccacggttg ctctatctgg caagcatggc
     2881 cggcgcccgg caatgttctc aactcacaac tcggtatgcg tgcttgaagt gcgaacgccc
     2941 gttgatggct gttgtcttcg ctgcccgtca gaatgatcgt cgggcaggcg atacgctcat
     3001 gatggttctc ggggtagggc tgcgccagtg ctttgaactg gtggatgatc gtctcgacgt
     3061 cggcgtgttg attgcgttct gtgaagaggt tcgcaaagaa gtgcgccagc ggagtagtgc
     3121 gaaaggcagc gcttagatct tcgaaagtgt aggcccagcg ataatcaata ccattggccg
     3181 tgtagttggc gatccttcct ttggtgaatt ccttgcttgg attgtaaccg gtgcccgaca
     3241 acacgagcgc ggcggtttgt tcgggacgaa ggtggtacat atgaggcgcc agcgacgagc
     3301 ccaccgagca gccgaccaga atcgcttttt cgccgggcag cgcatcatcg atggcttccc
     3361 aacaggcctt ggccatgtcg tccatggtga gcccagactg ggctttaggc gagcgcccgt
     3421 aacccggtat gtcgatggca atacaccggt accatgtcga gagatgcgcc atttgaaaaa
     3481 tccagcacga ctggtccatg gggttgggat gaataaaggc gatcacgggt cccacgcgtc
     3541 ccatccgctc gtagtaaaga ggtccatcaa tgtgctgcgc catgttactc ctccggtatt
     3601 aactgcggtg ttcgtgatcc gtgatactca gttcgctagc atatggccgc gatgcgagac
     3661 tcgacagtct gtggggttcg gcttgtgcgg acttgccttt ctatcggacc tcttttggtc
     3721 gataaaactg aatcgcgttg ttgtaaaaaa catcggctgc cttttcttcg cctaatgtct
     3781 ccagaaccag ggtgtagtct tcctcctgat acggtctggg cgcgcgggtg gacggcaggt
     3841 cggtgccgaa catgaggacc cgtggattgg ccgtataaag atcggtaaga gcggagcgca
     3901 cgtcgaaatc tacccgtccg aagccggtgg cctttacccg cacacctttt tccgcgagct
     3961 ttaggagggt cggaaaacct gctttggaca aaccgagatg gtcgatactg actgccggca
     4021 acgaaaccag cgtctcaaaa agatcggcaa gctcgctgga atcgacatac aattcggtgt
     4081 gccatccgac cagttcatga acgcgccgcg ccatgctctc cagatgatga atttcttccg
     4141 aaccgccgcg cttgacattg aaacggacgg cgcgaacacc tgcggcattg agttgctgca
     4201 actcctgatc ggatacagcc tgcggcactt gggtgactcc gacaaaacat gggccaagca
     4261 ctttcaaagc atggaacaga taactttgat caaacgcctg gaacgagccg gagacaaccg
     4321 caccgccgca aagatcgacc gctttcaagc gctctagata attctcgctg gtaaacgcgt
     4381 caggcatgaa tccttggtta ggcacgacgg gaaagttttt atcgatgatg tggaagtggc
     4441 aatcgaaata cttagctttg gagtccaatg attatctccc ctcaagtgct gcgaacatta
     4501 agaacgatgg ccagaggagg ctggataatt ccctactgcc cattcggtat agtcgatcag
     4561 cttgctggtg gcaagattca agcggcatga ttttttacgt caagtaccca cgcgcgagac
     4621 tcagcagaac ctcaaagaaa tcgtccggca aatacaggct catggcgcga tggtggcaat
     4681 cgccggcatt aagctcggtt tcttcactga tgaatacggt gcaatctttg aggagaccgc
     4741 ggtcgagtac ggcgcaatct acatcccaca cgtgatgaaa gggatttcaa tgacgcaaag
     4801 ctcaggagtg atcagattca ccctcacagc gccggttacc atctgatcgc cgagcgtatc
     4861 gccgagaaga tcaaacctct gttggatggg gctgaccggg taagaggccg
//
LOCUS       DASUTG010000701         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_257326, whole genome shotgun sequence.
ACCESSION   DASUTG010000701 DASUTG010000000
VERSION     DASUTG010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257326"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="trpB"
                     /locus_tag="VF089_16190"
     CDS             <1..1057
                     /gene="trpB"
                     /locus_tag="VF089_16190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6175549.1"
                     /translation="FHLHLREYAGRETPLYFAERLTEKLGGAKVYLKREDLCHTGANK
                     INNTIGQILLARRMKKARIIAETGAGQHGVATATVAARFGMQCEIFMGKEDVFRQSLN
                     VFRMKLLGARVRIVASGSKTLKDAMNEALRDWVTNVFDTYYLIGSVAGPHPYPMMVRD
                     FQSVIGQEARRQILKQEGRLPDCLVACVGGGSNSMGLFYPFVNDQNVRMVGVEAAGCG
                     LRSGKHAASVLGGEVGVLHGSKSYILQDNAGQIRETHSIAAGLDYPGVGPELSYLKDL
                     GRVEFASATDRAAMDAIQFLAQVEGIIPALESAHAIAAVMQLAPRLGKKKLIVVNLSG
                     RGDKDMGTVGDYLGVKL"
     gene            1144..1965
                     /gene="trpA"
                     /locus_tag="VF089_16195"
     CDS             1144..1965
                     /gene="trpA"
                     /locus_tag="VF089_16195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6175550.1"
                     /translation="MSRIQKKFLELKKADEAALIPFITAGDPDLQTTFEIMRAIEAGG
                     ADCIELGIPFSDPTADGPTIQRASERALRTQLTLPQILQMLRRFRATSEIPVILFGYF
                     NPFFRFGLKEFSRQSARAGADGVLCVDLPPEESEELKRWTDAEGMDLIFLLSPASGKE
                     RIELVARKGRGFIYYVSVTGVTGARQSLDQQLRYQVAQVRRLTSIPVGVGFGISTPQQ
                     AAWIVEFADAVVIGSALVQTIERASGRLAKAQAAGDFVSKIKRAMKRSRSNGRKA"
     gene            2011..3315
                     /locus_tag="VF089_16200"
     CDS             2011..3315
                     /locus_tag="VF089_16200"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX6175551.1"
                     /translation="MDAYLDTLNRIYNLRGGIIDLRLERMERALSLFDHPEQTFPSFH
                     IAGTNGKGSTAAMLQRILCSAGYRTALYTSPHLASFTERMRIDDQEITPDEVVALADE
                     IWQRTQPANILLTFFEIVTVMAFVYFARRQVDVAVIEVGLGGRYDATNLVRSIVSVIT
                     TISRDHEAFLGFDIASIAREKAGIIKKGVPVVCGALSPEAIEVISEIAQTNQAPCYRF
                     GQNFTLKLKPNGAFDYKGAKWNLPDLSVALHGAHQIRNACVATAALEVVDAQFPTSEV
                     AVRCGLQNVIWPGRMEMILTDPAVIIDGAHNKEGIEVLVDELRKLRGGRRVRMLFAVM
                     EDKDWRFMLQTLAEIVDEIVFTRVSQLERSADPVQLQQEMAGKLKCRVIADPRVAVET
                     LVQNAAPNELIVITGSLYLLGEVRPLLASMAQARQSEQSKRN"
     gene            3335..>3752
                     /locus_tag="VF089_16205"
     CDS             3335..>3752
                     /locus_tag="VF089_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX6175552.1"
                     /translation="MKPPKILLINILALWCWLGGADCGQAQTTQRSSGADDQINVSAD
                     KLSVSESGTQIEASGNVEVERQGTTLKAEQINVNRTTQDIEATGNISLDDPEWKIKSA
                     ESVRLNLGKETGEITNADVFIEQGHISISGRRFQKLG"
BASE COUNT          902 a          966 c         1045 g          839 t
ORIGIN      
        1 gtttcatctt cacctgcggg agtatgccgg tcgggaaact ccgctctact ttgctgaacg
       61 actcaccgag aaactaggtg gggccaaggt ttatctaaaa agagaggatc tttgtcacac
      121 cggcgcgaat aagatcaata acaccatcgg ccagattttg ctcgcgaggc gaatgaaaaa
      181 ggctcgaatc atcgcggaaa cgggtgcggg gcagcacggc gtcgccaccg caactgtagc
      241 ggcgcgattc ggcatgcaat gcgaaatttt catgggtaag gaagacgtct ttcggcagtc
      301 gctgaatgtc ttccggatga agctccttgg cgctagggtg cgcatcgtcg catccggcag
      361 caagaccctg aaagacgcga tgaatgaggc gttgcgcgat tgggtaacaa atgtttttga
      421 cacctactat ttgatcggtt ccgtggcggg cccgcatccg tacccgatga tggtgcgcga
      481 ttttcagtcg gtgatcggac aagaggcacg ccggcaaata ttgaaacagg aaggcagact
      541 tccggactgc ctagttgcct gtgtcggtgg cggcagtaac tctatgggat tattttaccc
      601 gtttgttaac gatcaaaacg tgcgaatggt gggtgtcgag gctgccggat gcggtctgag
      661 aagcggcaag cacgcggctt cagtcctggg tggtgaggtg ggtgtcttgc atggcagcaa
      721 gagttacatc ttgcaagata acgccggtca gatccgcgag acccattcga ttgccgccgg
      781 gttggactat ccgggtgtgg gacccgagtt gagctaccta aaggatctcg gtcgggtgga
      841 gtttgcgtcg gcaaccgatc gcgccgccat ggacgcgatc cagtttctgg cccaagttga
      901 gggaatcatc cccgcgcttg aaagcgctca tgccattgct gccgtgatgc aactggcacc
      961 gcggctaggg aagaaaaagt taatcgtcgt taatctatcc ggcaggggtg ataaagacat
     1021 gggcacagtg ggagattacc tcggagtgaa gttgtaggta ctcttcattt tacttcctgc
     1081 agatcttagc gccacggagc cgccggctct aagcttcaac tcaagcgaac aactggattc
     1141 aggatgagtc gaatacaaaa gaaatttctc gagcttaaaa aagccgacga agccgcgctc
     1201 attccgttta tcacggccgg agatccggat ctccagacga catttgaaat aatgcgcgcc
     1261 atcgaagcgg gtggagccga ttgcatcgaa ttgggaattc ccttctccga tccgactgcg
     1321 gacggcccga ccattcaacg cgcgtccgaa cgcgctttga gaactcaact tactctcccc
     1381 cagatcctgc aaatgctccg gcgatttcgc gccacttctg agattccggt gatactcttt
     1441 ggttacttca atccattttt tagattcggc ttaaaagagt tctcccgcca gtcggcgcga
     1501 gccggagccg acggtgttct gtgtgtcgac ctgccgccgg aagagagtga ggagttaaag
     1561 cgctggactg acgccgaagg aatggatttg atttttctct tgtctccggc cagcggaaag
     1621 gagcgaatag aacttgtagc tcgaaagggt cgcggcttta tttactatgt atccgtaacc
     1681 ggagtcaccg gggcgcggca atccctggac caacaattgc gttatcaagt agcccaagtg
     1741 cgacggctga cctcgatccc agtcggcgtt ggattcggca tctccactcc gcagcaagcc
     1801 gcctggatcg ttgaattcgc cgatgccgtg gttatcggca gcgcgttggt ccaaactatc
     1861 gaacgcgcca gcggccgctt ggccaaggcc caggcggccg gtgattttgt gtcaaagatc
     1921 aagcgggcga tgaaaagatc ccgcagcaac ggtcgaaaag cctagatctc cgctccttca
     1981 ggcaccctgt cacaggtgag tatcaaaccg atggatgcct acctagacac tctcaatcgc
     2041 atttataatt tgcgtggcgg cattattgat ctgcgtctgg agcggatgga acgggctctc
     2101 tcgctgtttg atcatccgga gcaaacattt ccttcttttc acattgccgg caccaacggt
     2161 aaagggtcca ctgccgcgat gttacagcga atcctctgtt ccgccggtta tcgcacggcg
     2221 ctttatacct cgccccatct ggcttcgttt accgagcgca tgcgcatcga tgaccaagaa
     2281 attacaccgg atgaagttgt cgcgctcgcc gacgaaattt ggcagcggac acaaccagct
     2341 aatattctcc tgacattttt tgaaatcgtg acggtcatgg catttgtata ttttgctcgc
     2401 cggcaggttg acgtagcagt tatcgaagtc ggacttggtg gtcgctacga tgccaccaat
     2461 ctagttcgct caatcgtatc cgtgatcacc acgatttcta gagaccacga agcttttctc
     2521 ggctttgata tagcttccat cgcgcgagag aaggccggca tcatcaagaa aggggtcccg
     2581 gtcgtttgtg gcgctctgtc gcccgaagcc atcgaagtaa tcagtgagat agctcagacc
     2641 aaccaggcgc catgttaccg attcggacaa aacttcactt taaagttgaa acccaacggt
     2701 gctttcgact ataagggtgc gaaatggaat ttgccggatc tctcggtggc gctgcatggg
     2761 gcgcatcaaa ttcgcaatgc ctgcgttgcc actgccgcgc tggaggtcgt cgacgctcaa
     2821 ttccccacca gtgaagtagc cgtccgttgt ggcttgcaaa atgtaatctg gcccggacgg
     2881 atggaaatga tcttaacgga tccggcggtg attattgatg gcgcgcataa taaggaaggc
     2941 attgaagttt tagtcgatga acttcgcaag ctacggggcg gccggcgagt aagaatgttg
     3001 tttgccgtaa tggaggataa agattggcga ttcatgctgc agaccctcgc cgagatcgtt
     3061 gacgaaatcg ttttcacccg agtcagtcaa ctggaaagaa gcgctgaccc ggtccagttg
     3121 caacaggaga tggctggtaa actgaagtgt cgggtcatcg cggatccccg tgtagctgtc
     3181 gaaactctcg tccaaaacgc cgcacccaat gaactaatcg tcatcacagg gtcgctttac
     3241 ctgcttggcg aagtacgccc actgctcgca agcatggcgc aggcacgaca atctgagcag
     3301 agcaaacgca actagattct cccgggcgta gaaaatgaaa ccaccaaaga ttcttctcat
     3361 caatattctt gccctatggt gttggttggg aggcgcagat tgcgggcagg cacaaacaac
     3421 tcagcgcagt agcggcgcgg acgatcaaat caatgtgagc gctgacaagt tatcggtcag
     3481 cgaaagcggc actcaaatag aagccagcgg caacgtcgag gtcgagcgcc aaggaaccac
     3541 acttaaagcc gaacaaatca acgtaaaccg gactactcaa gacatcgagg cgacaggaaa
     3601 tatttcactg gacgatcccg aatggaagat aaaatccgca gagtccgtcc ggctaaatct
     3661 cgggaaggaa actggtgaaa ttaccaatgc cgacgtgttt attgaacaag ggcatatcag
     3721 cataagcggc cgccggtttc agaaattggg cg
//
LOCUS       DASUTG010000702         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_849185, whole genome shotgun sequence.
ACCESSION   DASUTG010000702 DASUTG010000000
VERSION     DASUTG010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849185"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VF089_16210"
     CDS             <1..1240
                     /locus_tag="VF089_16210"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6175553.1"
                     /translation="WSSMSANPGASTVTPVPAPLDNIRVLDFTHVLAGPFCTRLLADL
                     GADVVRVESSKHPDHPWQSTYVGDDQRHASYLNTNRSKRSISIDLKNDAGQQIALKLA
                     AVADVVIENFSAGVMGRLKLDFVTIEELNPRIIYVSMSGYGHNGPRRDWTSMNMNLQG
                     YSGLMTVTGAEGDPPTAISNSWNDYIGGLHACFAVLQAIDKRAGSGRGSHIDLSQFEC
                     SAAMIGPLLLSSAVNGKVPLRLGNRSDVVAPQGVYRCAGADDWCAVSIENDQQWKALT
                     EVLANNELTSDSRLSSAAGRMDHHDKIDREVEAWTQQHSNTEVARRLQAAGVPAERVR
                     RIDQVIDEPDPATVFSRMEERRIGSMLTTRLPFTLSPVSLPGPRSAPSLGEHTADVLR
                     QWLNLSREQFDELEKQQAFT"
     gene            1237..1785
                     /locus_tag="VF089_16215"
     CDS             1237..1785
                     /locus_tag="VF089_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6175554.1"
                     /translation="MTQVDSSIITPELRAAIGRQSPARPLEILSASDVRRYIDATGDA
                     NRLWLDDEFARAAGYRARLLPPTLVGWVPFSFRESTERANADPMDLRRQLPLPPGYTN
                     VRNAGSETEWTAPVYLGEQLFTRSCIVDIFAREGRAGLGIYITQEEQVLNSNEQIVLR
                     RRHTLAVFPDKTYPDPPKTTDR"
     gene            1782..>2345
                     /locus_tag="VF089_16220"
     CDS             1782..>2345
                     /locus_tag="VF089_16220"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6175555.1"
                     /translation="MMRIIDLTGVIGSYATRLFAEEGHEVIRVEKAAGDDLRRMPPFL
                     TDKRDLEHSAYHQFLNAGKKSLTLDPHSASGRKLFIDLLARSDAVVAEQPLPVDEQLC
                     FEANPKLVITRIETDAPEICAMARSGLLSLTGQPNQAPMVLGAHIPYLAVGTYVGVAT
                     AAALLASKQSGCGIAATLSVGQSLESFV"
BASE COUNT          510 a          694 c          658 g          483 t
ORIGIN      
        1 atggagctca atgtccgcta atcccggcgc ttccactgtt acccctgtcc ctgcgccgct
       61 cgacaatatt cgtgtgctcg acttcaccca cgtactggcc gggccgttct gcacccggct
      121 gctcgctgat ttaggcgcag acgtggttcg ggtcgaaagc agcaagcatc ccgaccatcc
      181 ctggcaatcg acatacgtcg gcgatgacca gcgccacgct tcctatttaa ataccaaccg
      241 gagcaaacgc tccatcagca tcgatttaaa aaatgatgca ggacaacaaa tcgctctcaa
      301 gctagccgcg gttgcggatg tggtgatcga aaacttcagc gccggcgtta tgggcaggct
      361 caagctcgat tttgtgacca ttgaagagct caatccgaga attatttatg tcagcatgtc
      421 cggttatggt cacaacggcc cgcgccgcga ctggaccagc atgaatatga acctgcaggg
      481 atacagcggt ctgatgacgg tcaccggcgc ggaaggcgat ccgcccaccg ccatttccaa
      541 ttcatggaat gactacattg gcgggctcca tgcctgcttc gcggtcttgc aggcgattga
      601 caagcgcgcc ggcagcggcc gcggcagtca tatcgacttg agccagttcg aatgcagcgc
      661 ggcgatgatc ggacctctgt tgttgagcag cgcggtcaac ggcaaggttc cgcttcggct
      721 cggcaatcgt tcggacgtcg ttgctcctca aggggtttat cgctgtgccg gcgccgacga
      781 ttggtgcgcc gtgagcatcg aaaacgacca gcagtggaaa gcactgactg aagtgctggc
      841 gaataacgaa ttaacatcgg acagtcggtt aagcagcgcc gcaggccgca tggatcacca
      901 cgataagatc gatcgcgagg ttgaagcctg gacacaacaa cattccaaca ccgaagttgc
      961 acgacgtctg caagcagccg gagtcccagc ggaacgggtg cggcgcatcg atcaggtgat
     1021 cgatgaaccg gacccggcca cagttttttc acgcatggaa gagcggcgaa tcggttcaat
     1081 gctgacgacc cgactgccct tcaccctttc accggtctct ttgcctggcc cgcgcagcgc
     1141 tcctagcctg ggtgaacaca ctgccgacgt cctgcgtcag tggctaaatc tgtcgcgtga
     1201 gcagtttgat gagctcgaaa agcagcaggc tttcacgtga cccaagtaga ttcttccatc
     1261 attacgccgg aactgcgcgc cgcgattggc cggcagagcc ctgcgcggcc cctggaaatt
     1321 ctcagcgcgt ccgacgtccg gcgttatatc gatgccaccg gcgatgccaa ccgcttgtgg
     1381 ctcgacgatg aatttgcccg ggcggccggg tatcgcgccc ggctcctgcc accgaccttg
     1441 gtcggctggg ttccgttcag tttcagagag agcaccgaaa gagctaacgc ggatccaatg
     1501 gatctgcgac ggcagctacc gttgccgccg ggctatacca acgtgcgtaa cgccggatcc
     1561 gagaccgagt ggactgcacc cgtctatctt ggcgagcaac ttttcacgcg gagttgcatc
     1621 gtcgatatct tcgcccgcga gggccgcgcc ggattaggca tctatattac ccaggaagaa
     1681 caagttttga attcgaacga gcaaattgtc ttgcgccggc gccatacgct ggcggttttt
     1741 ccggataaga cctaccccga cccgccgaaa accaccgata gatgatgcgt ataatagatc
     1801 tcactggagt cataggatct tacgccaccc ggctttttgc cgaagaagga cacgaggtca
     1861 ttcgcgtcga gaaagccgcg ggtgacgatt tgcggcgcat gccgcctttt ttaaccgaca
     1921 aaagagattt ggaacacagc gcctatcacc aattcttaaa cgcgggcaag aagagcctga
     1981 cgctagaccc tcattctgcc agcggccgaa agctgttcat cgatttactt gcccgaagtg
     2041 acgcagtggt cgctgagcag ccgttgccgg tcgacgagca actttgcttt gaagccaatc
     2101 ccaaactggt tattacaaga atcgaaactg acgcgccgga aatctgcgcc atggcgcgat
     2161 ctggactgtt atcgttgaca ggtcagccga atcaggctcc gatggtactg ggcgctcata
     2221 tcccttactt ggcggtcggc acctacgtcg gcgtcgccac cgccgccgct ttgctggcat
     2281 ctaaacagag cggatgcgga atcgccgcga ccttgtcggt gggccaatcg ttggaatcat
     2341 tcgtc
//
LOCUS       DASUTG010000703         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1443418, whole genome shotgun sequence.
ACCESSION   DASUTG010000703 DASUTG010000000
VERSION     DASUTG010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443418"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VF089_16225"
     CDS             <1..1173
                     /locus_tag="VF089_16225"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX6175556.1"
                     /translation="GVGSSTEAATEKAVDQAFTAALAQAGIRKADMALVVFTAEHMRD
                     QEKLRNAVSHSSNHTVVAGSSAAGILTTEGEVEGSHGIAVLALASDQLQADAFLFESL
                     RGHDAEAGTKIAKLAGRSLDRNLLTVLMPDSYNGQAQEILQAMQREIGYSPVVGAGSS
                     ESGVAGATFQLCNDQVATNAVTGLHVSGSFESFIDITQGCQPITEPLTITRADGNIIY
                     EIEGRPAFEVFTSVLKGPLLEDLRRALMFVFVGLPADRRRNTVGGGEYLVRNIIGVDS
                     KKKIIGVADEVNEGQAIIFTLRDGQRARDDLGQMLKRQKQRLAGKKPAFGIYFNCCAR
                     GASLYGIAGIDSAYIRQVLGDFPLIGMFGGYELAPLGGANHLFAYTGVLALIAEEN"
     gene            complement(1266..>2367)
                     /locus_tag="VF089_16230"
     CDS             complement(1266..>2367)
                     /locus_tag="VF089_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6175557.1"
                     /translation="QRMGMGEAGQILGQLRGLEKFLRWGQRGMGDPSELDLDEIRNML
                     GEDAYEQLKYLKGVEQMLEEEGYIIRTGHGMRLTPKGMRKIGDKALREIFHMLNKNRW
                     GDHSTSARGSQGEQLEQTKAYEFGDPLNVNIGETLMNALESGNRSVPVRIRPEDFSVH
                     RNEFSTDSETVLCIDVSYSMLMNDALHAGKKVALALHRLIETKFPQDKLHLVAFRSNA
                     KIIRAEDLPSIVSLTYFMEHGTDIKEALRFARQLLGSNRAANRQIILITDGEPTAATL
                     DQGGRLHSGWGSAMLHGRIVRETLKEVRRCTQSGIKINTFMLGADFYRQGFVDQLSRL
                     NAGRVFYTSPDQMGNYIVVDYLANKRKRIGSR"
BASE COUNT          527 a          630 c          646 g          564 t
ORIGIN      
        1 ggagtaggaa gttccaccga agccgctact gagaaagcgg tcgatcaagc ttttaccgca
       61 gcgcttgccc aagccgggat tcggaaagct gacatggcac tcgttgtttt taccgcggaa
      121 cacatgcgtg atcaggaaaa acttcgcaac gccgtgtctc attcgtccaa ccacaccgtc
      181 gtggccggtt ccagcgctgc cggaattctc accacagagg gcgaagtcga gggctctcat
      241 gggatcgcgg tgcttgcgct cgcttccgac cagctgcaag cggatgcttt cttgtttgaa
      301 tcgttgcgtg ggcatgatgc ggaagcggga actaaaatag cgaaactcgc cggacgcagt
      361 ctggaccgca atcttcttac agtgctaatg cctgatagct acaatggcca agcacaggaa
      421 atactgcagg cgatgcagcg cgagatcggc tactcgccgg tagttggagc aggctcttcg
      481 gagagcggcg ttgccggcgc gacatttcaa ttgtgcaacg atcaggttgc cactaatgct
      541 gtcacgggtc tccatgtgtc gggctcgttc gaatcattta tcgacatcac gcagggctgc
      601 cagccgatca ctgagcccct gaccattacc agagccgatg gaaatatcat ctatgagatc
      661 gagggtcgtc cagcgttcga ggttttcacc tctgtgctca agggaccatt gctcgaagat
      721 ctcaggcggg cgttgatgtt cgtgtttgtc ggcctgccag ccgatcgccg gaggaatacc
      781 gtcggtggag gcgagtatct agtgcgcaac atcatcggcg tggattccaa gaagaaaatt
      841 atcggcgtcg ctgatgaagt aaatgaaggg caagctataa tcttcacgct gcgcgacggc
      901 caaagggcgc gcgatgatct gggacaaatg ctcaagcgtc agaagcaacg cctggccggg
      961 aaaaaacctg ctttcggtat ttactttaac tgttgcgcgc ggggtgcctc actctatggt
     1021 attgctggaa ttgacagcgc ttacatccgg caggtattag gcgactttcc cttaatcgga
     1081 atgtttggcg gatacgagct tgcgcctctg ggtggcgcca accatctttt tgcttacacc
     1141 ggagttttgg cactgattgc agaagagaat tgattggcga cttgaaatga gatccgagga
     1201 ctggctttac gacactccag atcggttcag atcttccaat gaatcgagca gattctgcgg
     1261 cttagctagc ggctgccgat ccgcttgcgt ttattggcca gataatcgac gacaatgtaa
     1321 ttgcccatct gatcgggaga ggtataaaaa acgcgcccgg cgttgagacg ggagagttgg
     1381 tcaacgaaac cctgccggta aaaatctgcg cccagcatga aagtattgat cttgatgccg
     1441 ctctgggtgc aacgcctgac ttccttgaga gtttcacgaa caatccgacc gtgcagcatc
     1501 gctgagcccc agccgctgtg caacctgcct ccctgatcga gagtcgccgc ggtgggttcg
     1561 ccgtcggtga tgagaatgat ttgccgattt gccgctcggt ttgaaccgag aagctgacgc
     1621 gcgaagcgaa gcgcttcttt gatgtccgtg ccgtgctcca tgaaataggt caatgaaaca
     1681 atcgacggca aatcttcggc tctgattatt tttgcatttg accggaatgc caccaaatgg
     1741 agcttgtcct gaggaaattt cgtttcgatc aaacgatgca gcgcgagagc caccttcttg
     1801 ccggcatgca aggcatcatt catgagcatt gaatagctga cgtcgatgca tagaaccgtt
     1861 tcgctatcgg tggagaactc gttgcgatga actgagaaat cttccggcct tatccgcaca
     1921 ggaacactgc ggttgccgct ttccagagcg ttcatgagtg tctcgccgat atttacattc
     1981 agaggatcgc caaactcata ggccttggtt tgttccagct gctcaccttg agagccgcgc
     2041 gccgaggtgg agtgatcacc ccagcggttc ttattgagca tgtgaaagat ttcacgcagc
     2101 gccttatcgc cgatttttct catccccttc ggcgtgagtc tcatgccgtg accggtccgt
     2161 atgatatacc cttcttcttc gagcatctgt tcaacgccct tgagatattt aagctgctcg
     2221 taggcatctt cgcctaacat attgcgaatc tcatccagat cgagctcgga cggatcgccc
     2281 atgccgcgct ggccccaacg gagaaacttc tccaaaccgc gtaactggcc gaggatttgg
     2341 cctgcttcac ccatccccat ccgttgc
//
LOCUS       DASUTG010000704         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_405035, whole genome shotgun sequence.
ACCESSION   DASUTG010000704 DASUTG010000000
VERSION     DASUTG010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405035"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..504
                     /locus_tag="VF089_16235"
     CDS             121..504
                     /locus_tag="VF089_16235"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6175558.1"
                     /translation="MQQALLVLMFLFAIAAVACGTPRDSASTGPALSGTTATTGRSKA
                     DLENAVKAKFKTDEQLRSADLGVSADSTNHEITLSGYVESEALRFKAVDLTRSVDSGL
                     TVNDQIEVKPSGRTADVLPNQQRHG"
     gene            473..1603
                     /locus_tag="VF089_16240"
     CDS             473..1603
                     /locus_tag="VF089_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX6175559.1"
                     /translation="MFFQTNSAMGERAKIRYAVVGLGHIAQAAVLPAFAHARQNSELT
                     AFVSDDPAKHEKLRGKYKVQHTYSYEEYEQCLSSGAIDAVYIALPNNLHCDFTVRAAQ
                     AGIHVLCEKPMAVTEEECEKMIAAAEQAHVKLMIAYRLHFEKANLEAIEIARSGKLGD
                     PRFFSSVFAMQVKEDNIRLKRELGGGTLYDIGVYCINASRSVFCAEPSEVHAFSVSSR
                     DPRFREVDEMTGALLRFPEERLASFTCSFGAADVSAYELVGTEGNLRVEPAYEYIGEL
                     KHRLTLRGKTRERTFSSRDQFAPELLYFSNCIINNVDPEPSGAEGLADVRVIRALYHS
                     AESGRPVQLEPYIKRQRPGMEQEITRPPVDKPELINVQSASQ"
     gene            1638..2264
                     /locus_tag="VF089_16245"
     CDS             1638..2264
                     /locus_tag="VF089_16245"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6175560.1"
                     /translation="MPNATQMIRQDHKKVEGLFKKFQQTKGAQAKRRLAENAMAELEV
                     HAALEEEIFYPAVKKEVDDGSMVQEALEEHQTVKQLISELKGMDEADEDFESQFSQLV
                     ENVQHHVEEEENEMLPKVEESELDLNSLGQQMSQRKQEMQSSGRATKKASNARSSRKS
                     AGAKSTGRKSAGKKSTTSKTAARKTGSAKKTSGRKATKKSGSRSRRRA"
     gene            2390..2944
                     /locus_tag="VF089_16250"
     CDS             2390..2944
                     /locus_tag="VF089_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6175561.1"
                     /translation="MADKPFLTDIKTLRERARQHIEQGAITPGYKADRDTVVKLLNEA
                     LATEIVCTLRYKRHYFMASGIHAQAVAQEFLEHANEEQGHADQIAQRIVQLGGAPDYS
                     PEGLVTRSHAEYVEGETLLDMIKEDLVAERIAIDSYREMAHYLGENDSTSRRLMEEIL
                     AVEEEHADDLVTLLQELGPDHGSR"
     gene            2998..3258
                     /locus_tag="VF089_16255"
     CDS             2998..3258
                     /locus_tag="VF089_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX6175562.1"
                     /translation="MLGWIGWIIFGVSVGIVGKFLMPGRDPGGFLVTILLGIAGALLG
                     GFVGRALGFYQEGEPAGFIMAVIGAIILLAIYRAVIGRRRTV"
     gene            3424..4377
                     /locus_tag="VF089_16260"
     CDS             3424..4377
                     /locus_tag="VF089_16260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6175563.1"
                     /translation="MLKIGFKLSSEEHRPADLIAQAQRAEQCGFSFAMISDHFHPWID
                     AQGQSPFVWPVIGAVAQATNKLVIGTAVTCPIIRIHPGIIAQAAATAAAMMPGRFILG
                     LGAGENLNEHIFGHRWPPVRVRHAMLKEAVEIIRLLWSGKQENFSGDYYQVENARIYT
                     LPDDLPPIALAASGSNAAELAGEIADGFVGTTADAELINVFDRAGGSGKPRYGEITVC
                     WAEDQNQAEETAYKLWPISGLNGPLMTELALPAYFEQAATMIDKTAVAKSVICGPDPE
                     RHWQAIQEYERAGYDHVFIHQVGPDQEGFFRFYERNILPRL"
BASE COUNT         1267 a         1321 c         1476 g         1027 t
ORIGIN      
        1 cgcggcgggg tcgggcgagg agaacgaggg gtaaacatcc agcagtgaaa gacggagttc
       61 gtggcatatt ttttgcccct tataggcata cggacgatag tttttggagg cgttttaacg
      121 atgcagcaag cattgctggt gctgatgttc ctgttcgcga ttgcagcggt tgcctgcggc
      181 acaccccggg actccgccag cacagggccg gcgcttagcg gaacgaccgc cacaacaggg
      241 cggagcaaag cggaccttga aaatgcagtt aaagccaaat tcaaaaccga cgaacagttg
      301 aggtcagcgg atcttggggt cagcgccgat tcgaccaacc acgagatcac tctctccggt
      361 tatgtcgaat ccgaggcatt gcgtttcaaa gcagttgatc tgactaggag cgtcgattcg
      421 ggattgaccg taaacgacca aatcgaagta aaaccgagtg gtcgcaccgc tgatgttctt
      481 ccaaaccaac agcgccatgg gtgaacgagc aaaaatcagg tacgccgtgg tcgggttggg
      541 gcacatcgcg caagctgccg tattgcctgc atttgctcat gcccgtcaga attccgagct
      601 aactgctttc gtgtcggatg atccggccaa gcatgaaaag cttcgcggta agtacaaagt
      661 acaacataca tactcgtatg aggagtatga gcagtgcttg agcagtggcg caatcgacgc
      721 cgtgtatatc gcgctgccta acaacttgca ctgtgacttt accgtgcgcg ccgcacaagc
      781 cggtattcac gttctctgtg aaaagccgat ggccgtcacc gaggaggagt gcgagaagat
      841 gatcgcggct gccgagcagg cgcacgtgaa gctgatgatt gcctaccggc tccatttcga
      901 gaaggccaat cttgaagcta tcgagatcgc gcgctccggc aaactcggtg acccgcgatt
      961 cttctcgtcg gtatttgcga tgcaagtcaa ggaagataac attcgtttga aacgggagct
     1021 cggcggcggc acgctctacg atatcggcgt gtactgcatc aacgcgtcac gctccgtgtt
     1081 ttgtgccgag ccctcggaag ttcacgcgtt cagtgtcagc agtagagatc cccgcttcag
     1141 ggaagtggat gagatgaccg gcgccctctt acgttttccc gaagagcgac tggcatcatt
     1201 tacttgcagc ttcggagccg cagacgtctc cgcctatgag ctggttggca ccgagggcaa
     1261 ccttcgcgtt gagccggctt atgaatacat aggagagctc aagcaccggc tcaccctcag
     1321 gggtaaaacc cgcgaacgga ctttttccag ccgcgatcaa tttgccccgg aactactcta
     1381 tttttccaac tgcattatca ataatgtcga tcccgaaccc tccggagcgg aaggactggc
     1441 cgacgtgcgc gtcattcgcg cgttgtacca ttcggccgag agcggcagac cggtacaact
     1501 ggaaccctat atcaagcggc aacggccggg gatggaacaa gaaattaccc gacctccggt
     1561 agacaaaccc gaactgataa acgtacagag cgcatcgcag taagcaagca acggtcacta
     1621 acaatggagg aacaaatatg cctaatgcaa ctcagatgat tcgccaggac cacaagaaag
     1681 tcgaagggct cttcaagaaa ttccagcaga ccaaaggggc gcaagccaag cggcgcttgg
     1741 ctgagaatgc catggccgag ctcgaagttc acgcggcgct ggaggaggag atcttctacc
     1801 cagcggtgaa gaaggaagtt gatgacggct cgatggtgca ggaggccctc gaagaacacc
     1861 aaacggtcaa acaactgatc agcgagctta aagggatgga cgaagcagac gaggatttcg
     1921 aatcgcaatt ctcccaactg gtagagaatg tgcagcacca cgttgaagaa gaagagaacg
     1981 agatgttgcc aaaggtcgaa gaaagcgagc tggatcttaa ttcgctgggc cagcagatgt
     2041 cacaacgaaa gcaggaaatg cagagcagcg gtcgagcaac taaaaaggcg tcgaatgcca
     2101 gatcgagccg gaagagcgcc ggtgccaagt ccacagggcg gaagtctgcg ggcaaaaagt
     2161 cgacaaccag caagactgct gcgcgtaaga cgggcagcgc gaaaaagacc agtggacgaa
     2221 aggcgacaaa aaaatccgga agccgctcca ggcggcgagc ttgatcagtt gcggcgtttt
     2281 gatttcgatg acgctgccgc ataggaacag aagccacagc gaatatcacc ggcttgcgct
     2341 gtagggggta tgtgataagc gaagatgagc tcattccgag ggggagtata tggctgataa
     2401 gccttttttg accgacataa agacgctccg ggagcgggcc cggcagcata tcgagcaagg
     2461 cgctatcaca ccgggttaca aagccgatcg cgataccgtg gttaaattac tcaatgaagc
     2521 gctggcgacg gagatcgtgt gcacgctgcg ttacaagcga cattacttca tggcttcggg
     2581 catccatgct caggcagtgg cccaggagtt tctcgaacac gccaacgaag aacagggaca
     2641 cgccgatcaa atcgcccagc gtatcgttca gctcggcggc gctcctgatt attcgcccga
     2701 gggcctagtg acccgcagtc acgccgagta tgtcgaaggc gaaacccttc tcgacatgat
     2761 taaagaggat ctggtagccg agcgcattgc catcgacagc tatcgggaga tggcgcacta
     2821 tctgggcgaa aacgattcca ccagccgtcg tctgatggaa gaaatcctgg cggtggaaga
     2881 agagcacgct gacgatctcg tcacgctact acaggagctg ggcccggatc acggatcgag
     2941 ataaacaatg aactattcaa tcagctagac aaacctcaca gaagaaagga aacgactatg
     3001 cttggatgga tcggctggat tatcttcgga gtgagtgttg gaattgtcgg gaaatttttg
     3061 atgcccggac gcgatccggg aggatttctt gtcactatcc ttttgggtat cgccggcgcc
     3121 ttgctgggtg gcttcgtcgg cagggctctc ggcttctatc aagaaggcga gccggctggc
     3181 ttcattatgg cggtgattgg cgcgattatt ctgttggcga tttatcgcgc ggtaatcggg
     3241 cggcgccgaa cggtttaagt cagccgttaa ccgcttcata tccgacgttt agggttcaag
     3301 gctcagtagc ggctagttct caaggctgat gtttgagcct tgatctctta acgttgaacc
     3361 tcagtaaact ttccaagcgg cctcccactg accgttctcc agcaggcctg cggagaagtg
     3421 tgcatgctca aaatcggatt caagctgtcg agcgaggaac accggccagc ggacttgatc
     3481 gctcaagcac agcgagcgga gcaatgcgga ttcagcttcg cgatgatctc agatcacttt
     3541 cacccgtgga tcgatgcgca aggccagagc ccgtttgtat ggccggtgat cggcgccgtc
     3601 gctcaagcga ccaacaagct ggtgattggc acggcagtga cttgtccgat catacggata
     3661 catcccggca ttattgcgca agctgcggct acggccgctg ccatgatgcc ggggcgcttc
     3721 atcctcggcc tgggagccgg ggaaaatctt aacgaacata tatttgggca tcgctggccg
     3781 ccggtgcgcg tccgtcacgc gatgctcaag gaagcggtgg agatcatccg cttgctttgg
     3841 agcggcaagc aggaaaattt ctccggcgac tattatcagg tggagaacgc acgcatttat
     3901 actctaccgg atgatctacc gccgattgcg ctggcagcca gcggctcaaa cgcggccgag
     3961 ctggcgggag aaattgccga tggttttgtc ggcacgacgg cggacgcaga gctaattaat
     4021 gtgttcgatc gagccggcgg cagcggaaag ccacgctacg gcgagatcac cgtctgctgg
     4081 gccgaagatc aaaatcaagc ggaggaaact gcctacaagc tgtggccgat ctcagggctg
     4141 aacggaccgc taatgactga gttggccttg ccggcctatt tcgaacaggc agctaccatg
     4201 atcgataaaa ccgcggtagc aaaaagcgtc atctgcgggc ccgatcccga acgtcattgg
     4261 caagcaattc aggagtatga gcgcgccggt tacgatcacg tgtttatcca ccaagtcggt
     4321 cccgatcagg aaggtttctt tcgattctac gagagaaata ttctgccacg gctctaaaga
     4381 cgctgttctt atagcgcttg cgccctcgcg gcaatgtaga agttcctgcc cgggacatct
     4441 tcgttacggg tagcttcgca cactaatttc tggatctcac tccggtgcgc gccgccgaga
     4501 tgaaataaac ctaaagtttc gcctgcagca ttaaagagcg ccgggcaggc tcgcgggcat
     4561 cactcggtct accaaagcag caggcacttt caggcacact gtgtttcggc gctctgttgc
     4621 ttccaatacc tagcagggtt gctgaaaaaa gcctcttgca ggctgctcaa aaagatccag
     4681 atgcgaggcg cgcgaaaaat cgacgaaagg cgtgcttacg gtacgttgag cgaggcgatt
     4741 gagcggcaac gaagcgtacg gggtcttttt cagcaggcct gctagaagct tcgtcccatt
     4801 tgcggaaggc actcacatct actttacccg ggcttgggac agaatccgca accgtactca
     4861 attcatcgga ttggtttgtc gagaatgcaa caataaaatt tctggatacg tggcatatcg
     4921 gttgcacaag tattcgcgga cgcaacgcca caagtcatac gaaaggagaa aaactgatgc
     4981 agcgaaaact gttaattcta tcgggaatta ttttcagcgg aacgattgca cttaacggca
     5041 acctggtgtg gtcacaaacc aaagggggaa gcgggacgcc gggcgccgcg g
//
LOCUS       DASUTG010000705         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1145227, whole genome shotgun sequence.
ACCESSION   DASUTG010000705 DASUTG010000000
VERSION     DASUTG010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145227"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..650)
                     /locus_tag="VF089_16265"
     CDS             complement(195..650)
                     /locus_tag="VF089_16265"
                     /inference="COORDINATES: protein motif:HMM:NF025032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX6175564.1"
                     /translation="MGESFGRISNGGFLLLMFLFGCSAGGGASLPSASSPLVGRWRQT
                     SIATDNQSAKCPATMPLPGGSTTSCSGNDMIEFHPNGTFTATFSGSSVKGSGTWRLTG
                     DNLSLTFTAPPNVAGRSQAATIRFDEGAKTININSKLGETPTTETYTRQ"
     gene            823..1266
                     /locus_tag="VF089_16270"
     CDS             823..1266
                     /locus_tag="VF089_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175565.1"
                     /translation="MRTTWLLMVCALFFGASLAQGAEYFIYRDSAGTIVLSNIRPTAE
                     AEVLERYDLQEVSEEALRAAREREHSFWLGLQDEELARSNRELAESNHRLAEAITTNA
                     ALRETAPGVLVQVASSHGSRFQRPHTDPRQTRAHSQTGFRHTKIR"
     gene            complement(1231..2985)
                     /gene="ybaL"
                     /locus_tag="VF089_16275"
     CDS             complement(1231..2985)
                     /gene="ybaL"
                     /locus_tag="VF089_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX6175566.1"
                     /translation="MPHSVTLISTIAAGLGLALIMGFIAARLKLPVLVGYLTAGIIIG
                     PATPGFVADVELSSQLAEIGVMLLMFGVGLHFSLDDLLAVRRIALPGAVVQITVATVL
                     GMAVSATWGWSLAAGLVFGLALSVASTVVLLKALEHRGVLDSVNGRIAIGWLIVEDLV
                     MVLVLVLLPAMSVWLGGNVNPAEPAGDGTLLATFGLTVGKVAVFIVLMLVAGRRLFPW
                     LLWQIARTGSRELFTLCIVAAAVGIAYSSAELFGVSFALGAFFAGMVMRESPLSYRAA
                     EESLPLRDAFSVLFFVSVGMLFDPDVLIREPFHVLIVLAIIIFGKSLAAFFIVLLFRY
                     PLNTALTVSASLAQIGEFSFILVGLGVSLGLLPTEGQSLILSGAIISIALNPVLFGAI
                     EPVQAWIRSRSKLARALERPDDPLAALPMTTDLQRLTGHVVIVGYGRVGRRIGEALTA
                     KGISIVVAEQNREIVERLRSNGIAAVSGDASEPAVLLQAHVHRASTLVIATPDTLNVR
                     KMIEVARTVNPRIQTVVRTHSEEEAVLLEKEQAGKVFLGEHELAIGMIQHVLECHAAK
                     AEHVNKSSANFRMPKPRL"
     gene            3698..4924
                     /locus_tag="VF089_16280"
     CDS             3698..4924
                     /locus_tag="VF089_16280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175567.1"
                     /translation="MERKWWLVIVGCITHAVNTGLSYFGMSAYFPSFEREFGWSRTAI
                     SGAFSLARIESGLLGPMEGYVTDRVGPQRMIFIGLFLCVGGFFALSFVDSLPMLYVVI
                     VLGIVLGSSLGYNMPISVLIAKVFHERRSLAFGIFRMGPGISGPMVPLVGWMIGLWGW
                     RSAAVISGFVLLAVGLPLALVIKRIYSQEEAANVVAEKADAISKDSPRSSTDPQFTLK
                     QALHHRSFWLLSVAMGMRHLVTEGVSVHFVILLVDRGWGTEAASTLLGISALIGAPAR
                     IGMGWLGDLLDKRRLVMGLLLALSVSVLLMGYTAHAVVFTTCMIIYSLAYGGLAALQE
                     PIRADYFGTRAFATIQGMSRSVVTAGTFLGPIIAGFFYDVTKSYTIAFGVFALVSLVA
                     TLFMFFAIPPQRACGD"
     gene            5082..>5963
                     /locus_tag="VF089_16285"
     CDS             5082..>5963
                     /locus_tag="VF089_16285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175568.1"
                     /translation="MRQNRFPVIDGDGHITERSKEILEFLGGNYRGTSKDQSWADTFS
                     PFPSLDGWARLSGMSSPGLRDYPDCDTWISFLDECGIQMTIVYPTAALAFGLIQDPAW
                     AVDIARAYNSWLHASYTKRTSRILGAALIPIHDVPAAVKELRRVKEELGMPAAMLPSV
                     TNLRKGYGHESFFPIYEEACRLDMPLAVHGAPSRGLGFDFLETFAEVHALEHPLPLMI
                     QLTNMVFQGVFELYPDLRVAYLEAGAGWVPFMMDRLDEDFERRGKKWAPRLKRLPSDY
                     ISGGSVYVSLESEERTLP"
BASE COUNT         1302 a         1641 c         1682 g         1338 t
ORIGIN      
        1 ccgtgtccgt caattttgaa tcaggttatt aagcgacgca tgtagctaag tctctgaatt
       61 catcaagatc aattgactta tgcgttctgc tcgaagccga cagaagacta cagattttca
      121 ctctcctcga gctttgttat ctcgacaacc cgagtaatca gctgagcact acctgcaccc
      181 agatcgcgga gtgattattg ccgcgtgtat gtttcagtgg taggtgtctc ccccaatttg
      241 gagttgatgt ttatggtctt ggcgccttcg tcgaatcgga tggttgccgc ctggcttctg
      301 ccggcaacat tcggcggtgc ggtgaaagtt aatgaaagat tatctccggt gagccgccag
      361 gttcccgaac cctttacgga gctgccggag aaagtggctg tgaacgtgcc attcggatga
      421 aattcgatca tatcgttgcc gctacaggag gtagtcgagc cgccgggcaa cggcatagtg
      481 gcagggcatt ttgccgactg gttgtcggtg gcaatcgatg tttgacgcca tcggccaacc
      541 agcggggatg aggcagaagg taggctggct cctcctccgg cactacagcc aaacaggaac
      601 atcagcagca ggaaaccccc gttcgaaatt cggccaaagc tttcgcccat cgatctttcc
      661 ttccactcct gcctaacgca tcgcgcgccc tttcttctta actcgataga tcgctcttgc
      721 caatggctct aggaccccga ggagttcttt ccggatcaag tattgaccca ggctaatgcg
      781 ccggttaggc atattaaata agatcacaag gagggcatca cgatgagaac aacgtggtta
      841 ttgatggttt gcgctttgtt ctttggcgct tcactggcgc aaggcgccga gtactttatc
      901 taccgcgatt ccgccggaac gattgtcctg tccaatatcc gtccaacagc cgaagcagaa
      961 gtgctcgagc gttatgactt gcaagaagta agcgaggaag cgctgcgcgc cgcccgcgag
     1021 cgtgagcatt cgttctggtt gggactccaa gatgaagaac tcgcaaggtc taaccgggag
     1081 ctggcagaaa gcaaccatcg tttggctgag gcgatcacca cgaacgcagc gctgcgtgag
     1141 accgcgccgg gggtcttggt tcaggttgca agttcacatg gctctcgatt ccagcgcccg
     1201 cacacggatc cgcgacaaac ccgggcgcac tcacagacgg ggtttcggca tacgaaaatt
     1261 cgctgagctc ttattgacgt gctcggcttt ggccgcgtgg cattcgagca catgctgaat
     1321 catgccgatc gcgagttcgt gctcgccaag aaaaactttg ccggcctgct ctttttccaa
     1381 gagcacggcc tcctcctcgc tgtgcgtgcg cacaaccgtc tgaatgcgcg gattgacggt
     1441 gcgagcgacc tcaatcattt ttcgcacatt tagggtgtcg ggagttgcga tcaccaaagt
     1501 cgatgcccgg tgaacatgcg cctggaggag aaccgcgggc tcggaggcat cgcccgacac
     1561 tgccgctata ccgttcgagc gcaagcgctc aactatttcg cggttctgct cggcgacgac
     1621 aatcgatatg cccttcgcag tcagcgcctc gccgatgcgg cggccgaccc ggccgtatcc
     1681 gacgatgacc acatgtccag tcaggcgctg tagatcggtg gtcattggca aggccgcaag
     1741 agggtcatcc gggcgctcca aagctcgagc gagttttgag cgggagcgga tccaagcttg
     1801 caccggctcg atcgctccga acaacacagg attgagagcg atcgaaatga tcgcgcctga
     1861 caggatcaga ctctggcctt cagtgggcag taacccaagc gataccccga ggccgacgag
     1921 gatgaacgag aactcgccga tctgcgccag gcttgccgag acggtcagcg cggtgttgag
     1981 cggatagcgg aacagaagga cgataaaaaa tgccgccagc gatttgccga agatgatgat
     2041 cgccagcaca atcagaacgt ggaacggttc gcgaatcagc acgtcgggat cgaacaacat
     2101 accgacggac acgaagaaca gaactgagaa tgcgtccctg agaggcaagg attcttcagc
     2161 cgcccggtag ctgagagggg attctctcat caccatgccg gcgaaaaacg cgccgagcgc
     2221 gaatgagacg ccaaagagct cggcggaact ataagcaatg cccactgcgg ctgcgacgat
     2281 gcacaaagta aaaagctcgc gagagccggt gcgagcaatc tgccatagca accagggaaa
     2341 cagacggcga ccggcgacca gcatcagtac gataaacacc gccaccttgc cgacggtcag
     2401 cccaaacgtc gccagtaagg ttccatctcc tgcaggttcg gcgggattga cattaccgcc
     2461 cagccacacc gacatcgctg gcagcagcac cagcaccaaa accatgacga gatcctcgac
     2521 gatgagccag ccgatggcaa tgcggccatt gaccgaatca aggacaccgc gatgctccaa
     2581 ggctttgaga agcacgaccg tgctggctac cgagagcgca agaccaaaca ccaacccggc
     2641 ggcaagactc caaccccaag tagcggagac agccattccc agtaccgtgg cgaccgtgat
     2701 ttggacgacc gcgccaggta atgcgatccg gcgcaccgcc aataagtcat caagtgaaaa
     2761 gtgcagcccg acgccgaaca tcaacaacat cacaccgatc tcagcgagct ggctcgatag
     2821 ttcaacgtcg gcaacaaagc ccggggtcgc cggcccaatg ataatgcccg cagtgagata
     2881 accaacgaga accggcagct ttagcctggc tgcgataaag cccataatta atgcaagccc
     2941 caagccggcg gcgattgtgc tgattaaggt aacgctgtga ggcacgtagc tattctccta
     3001 gcaggctact gaaaagactc atattgtagg ttgggctcga tcgtctcgtt acaacgtact
     3061 cgctaagcgc ttccgctcgc tgattcctcg cgcctcggct ctaacttctt ttgagcagcc
     3121 tgcaaattcg cattttacat caaccctgca gattggaatg tcgagggaca tgactttgcc
     3181 aaatccggcg gccctcgaat cgcacaaaaa tcggcctgac ttgcggcaat taaacgtctt
     3241 gggcgccgtt tccggatcga tgattgccta cgccctggag aatcagcaca gagcgttcga
     3301 acagctattt tatcgcggat tgagcgtgct gatcagcttg aatggccatc agacagcggc
     3361 agttcgttca atggttcgtg tccggtacag cgacacagaa cgaaagtata atgtatacgt
     3421 tatgctcgca atctctgcag gtgaaacatc gacaagttcc aaggacgaat acgcagttca
     3481 ttggtcgaaa tatctgcttg gggttgggac atgaatacca gcagattgat tgcgcccatt
     3541 ttctcgcaag atcgtctgca aacacctcgg tcgggtcatt gcgtttgagg atcgcttgcg
     3601 cgagctattt cgtctcggtt tactttctta cccggacgat ttcagttatc gtgcgaacgt
     3661 tttagcaact tcgctctaac ctcggaggtc ggtccggatg gaaagaaagt ggtggctcgt
     3721 gatcgtcggt tgcatcaccc atgcggtgaa taccggcttg agttactttg gcatgagcgc
     3781 ctactttcct tcttttgagc gcgagtttgg ttggagccgc acggcaatct cgggcgcttt
     3841 ttctctggcg cgcattgaat ccggccttct cggtccaatg gaaggatatg tcacagaccg
     3901 ggttggaccc cagagaatga tatttatcgg gctgtttctc tgcgtcggcg gctttttcgc
     3961 gctgagcttt gtcgactcgc tgccgatgct ctatgtcgta atcgttctcg gcatcgttct
     4021 cgggtccagt ttaggctaca acatgccgat tagcgttttg atcgccaagg tctttcacga
     4081 gagaagaagc cttgcctttg gcatctttcg catgggcccc gggatttcgg ggccaatggt
     4141 tccgctggtt ggatggatga ttggtttatg gggatggcgt agcgcggcgg tgatatccgg
     4201 gtttgttctg ctggcggttg gattaccgct tgccctggtt atcaaaagaa tttactccca
     4261 agaagaagcc gccaatgtag ttgcggagaa agcggacgcg atttcaaaag actctccgcg
     4321 ttcttcgacc gatccgcagt tcactttaaa gcaagcatta catcatcgat ctttttggtt
     4381 gctttcggtc gcaatgggca tgcggcatct ggtgaccgaa ggagtttcgg ttcactttgt
     4441 cattctcctg gtcgaccgcg gctggggcac tgaagcggcc agcacattgc tcggcatctc
     4501 cgcattaatc ggcgcgccgg cccggatcgg catgggctgg ttgggagatt tgctcgataa
     4561 gcgccgattg gtgatgggat tattgttggc gctgagtgtc tccgtattgt tgatgggcta
     4621 taccgcccat gcggttgttt ttaccacgtg tatgattatc tattcgctgg cgtacggcgg
     4681 actcgccgct ttacaagaac cgattcgcgc cgactatttc ggcacccgag cattcgcgac
     4741 cattcaaggc atgagccgat cggtggtaac tgcgggcact tttctcgggc cgatcattgc
     4801 gggatttttt tacgatgtga cgaaaagcta caccattgca ttcggcgttt tcgctctcgt
     4861 cagtttagtg gcgacgctgt ttatgttttt cgccatcccg ccgcagcgag cttgtggcga
     4921 ttgatccgct gcgattacgg cacagggaag tcggtgcgct gcggtcgggc gatgatgtac
     4981 gtgggcatcc ggagcttgac tcaggagaac tcaagcaata catacgacgc tggtaatcga
     5041 atctataatt cgtataaatg cccgaaaggc ggtacgttgc gatgagacag aatcgctttc
     5101 ctgtaatcga cggcgacggt cacattacgg aacggagcaa agagattctc gagtttctcg
     5161 gcggcaacta ccgcggcacc agcaaggacc agagctgggc cgatacgttc tcgccgtttc
     5221 cctcgttgga tggttgggcg cggctttcgg gcatgagctc gccggggctg cgcgattatc
     5281 cagattgcga cacctggatc tcgtttctcg atgagtgcgg catacagatg accatcgtct
     5341 accccacagc cgccctcgcc ttcggcttga ttcaggatcc ggcctgggcg gtggacatcg
     5401 cccgcgctta taattcttgg ctgcacgcga gttatacaaa gcgaacttcc cggatcctgg
     5461 gcgcggcgct gatcccgatt cacgatgtgc ccgccgctgt caaggagttg cgccgggtca
     5521 aagaagaact gggcatgccg gcggcgatgc tcccatcagt caccaacctg cgcaaaggct
     5581 acggtcacga gagtttcttt ccgatctacg aagaggcttg ccgcctcgac atgccgctcg
     5641 ccgttcacgg cgcgccaagc cgcggcctgg gatttgattt tctcgagacc ttcgccgaag
     5701 tgcacgccct ggaacatcct ttgccgctta tgatccagct caccaacatg gtattccaag
     5761 gggtgttcga gctttatccg gatctaaggg tcgcctatct cgaggccgga gccggttggg
     5821 tgccgtttat gatggaccgg ctcgatgaag acttcgagcg gcgcggcaaa aagtgggcgc
     5881 cgagactcaa gcgcttgccc agcgactata tatccggcgg ttcggtctac gtttccttgg
     5941 aatccgagga gcgtaccctg ccg
//
LOCUS       DASUTG010000706        12952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_110126, whole genome shotgun sequence.
ACCESSION   DASUTG010000706 DASUTG010000000
VERSION     DASUTG010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110126"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VF089_16290"
     CDS             complement(<1..503)
                     /locus_tag="VF089_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX6175569.1"
                     /translation="MNPREWLFVAEAWAAGGAEHHSPEISGIIFPFLNFLIYAGIIYY
                     FAVPLIRSFLKSRHDEVLAMVTGAAARKRSAETLVEDYRSRLATLNQEIDSIQASLKA
                     EGERDKVRLLREAEALAAKVKSDAQLLANQEVKAAKHQVLVELAERAKASATDLIRRH
                     LSQADQS"
     gene            complement(500..928)
                     /locus_tag="VF089_16295"
     CDS             complement(500..928)
                     /locus_tag="VF089_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX6175570.1"
                     /translation="MITIDFSVVWQILLFLGLWLIVSKLLFWPYMTLLEEREQKTSGA
                     DESAYHLEHEAERLRSQYEEALANAAAAGNATKEAIVQQARQQREVILSSARGEAAGI
                     LERVRQEVQSQLAQEREVAIREADAVAHDMVSKILGRRVG"
     gene            complement(1075..1575)
                     /locus_tag="VF089_16300"
     CDS             complement(1075..1575)
                     /locus_tag="VF089_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6175571.1"
                     /translation="MPAQSTLEAEAPLVLAGPKMKAEPVPTPSEPRLVGQLHKGSRIT
                     GQLAFEGSVRIDGSVEGEIRCLETLTIGEGAEVRARISSRVVIIRGKVEGNVTAKDKV
                     ELLAPGRLFGNVSTPRLIITEGVVFDGDCSMGAAKQAGVASSKSLNTEKVAVAQASKL
                     QPDSSK"
     gene            2048..2614
                     /locus_tag="VF089_16305"
     CDS             2048..2614
                     /locus_tag="VF089_16305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6175572.1"
                     /translation="MILASVAIAAQTADKKTPVYATFKTSMGDIVVELFEDKAPKTVA
                     NFIGLATGTKEWTDPKTGEKVKRPLYNGTIFHRVIPGFMIQGGDPLGNGTGGPGYRFE
                     DEFHPELRHNKSGILSMANAGPHTNGSQFFITHKATPWLDGKHTVFGEVIKGQNVLDA
                     IANVGRDFRDKPMKEIVIQQVNISRGSY"
     gene            complement(2641..3714)
                     /locus_tag="VF089_16310"
     CDS             complement(2641..3714)
                     /locus_tag="VF089_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6175573.1"
                     /translation="MITNPQQTTLVDHRKLTRFVAAAFKKLGVPRQDAAIAANVLVMA
                     DLRGVDTHGVIRFNPGSWYVKWLRDGEMTPEPKIRIVSDAGSSALLDGDRGIGMVVGH
                     RAMELAIEKAKVSGVGIVGVCNSRHFGMCAYYAMQALPHDMIGIAMTNAGRQVVPTFG
                     SEARFGTNPICFAAPAQKELPFVIDMATTTAAAGKLEVAARRGASIPLGWALDEKAEP
                     TSDPRIAQKARRLLPLGGTREGGSHKGYGLAILVEILCGVLTGTVTALNQNQDPRGHF
                     FVAIRIDAFRPVSEFKSDMDRLITELKSTRPIEGQERVYVAGEIEFETAKRRAEEGIP
                     LLGSVLRGLRDLSEELEITYDLE"
     gene            complement(4289..4849)
                     /gene="msrA"
                     /locus_tag="VF089_16315"
     CDS             complement(4289..4849)
                     /gene="msrA"
                     /locus_tag="VF089_16315"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6175574.1"
                     /translation="MTQNNGVAVFGGGCFWCTEAVFDELRGVTSVVSGYAGGELKNPT
                     YEQVCGGRTGHAEVIRIEFDPDQIAFRDLLTVFFATHDPTTLNRQGNDVGTQYRSAIF
                     YADDEQKREAENFIKELEAAGSYSKPIVTTLEALTDFYEAEDYHQKYYANNPYQPYCQ
                     VMIPPKLNKLHKQFAELLKSHSKGAS"
     gene            5508..6980
                     /locus_tag="VF089_16320"
     CDS             5508..6980
                     /locus_tag="VF089_16320"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6175575.1"
                     /translation="MAKLLIASEQVDSESNETTAVKNPATGELVDSVPKGTVNDVRRA
                     IDAAKGALKKWSEMAPSKRGAILLQAGHAILQQEKEIATLLTREQGKPMRESILEIRR
                     FVHTLDHYGGMAKSLRNAAVMLDVGRHGLVLRKPLGVCGSIVPWNFPVSLMGNKLGPA
                     LLMGNTVVVKPAGTTPLTDLRCCEMIDRAIQDAGGPRGVLNVVTGPGSVVGEELLVNP
                     TVRKIGFTGATDTGRRVMQSAAKDFKHVTLELGGSDPMIVCDDADIDRAVSAASVGRF
                     FNCGQACLAVKRLYLFDRIADSFMDKLVEKVKRISVGNGLKAGVLMGPLHTAEQRREV
                     EEQVEDAVKRGAKVLYGAQRPKGDDYEKGFYLQPTLVADVDPASRLLQEECFGPALPI
                     VRVKNLDQAIEQANNSIFGLGSSIWTRDINRAMAGAERIEAGYTWVNSAQIIYDELPF
                     GGVKQSGLGKEHGEEAIEHYTDSKSVVIATETHSEMVGGE"
     gene            7043..8584
                     /locus_tag="VF089_16325"
     CDS             7043..8584
                     /locus_tag="VF089_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX6175576.1"
                     /translation="MGVELPEIYNAATTFVDENITQGRGGRVAIYYQDEAITYQEVYE
                     KVNRTGNALKELGVEIEHRVLLALPDSPEFAYSFFGAIKIGAVPIPTNPWMAAKDYEY
                     LINDSRARAIIVHESVLPEVAKIWNNTRYLKQILVVGKGKDRARSYQELIARASDYLE
                     PEPTHCDDVGFWGYTSGSTGMPKGAVHLQHDMITITDLFVKPILGMNENDRCFSASKL
                     FFSYGLGNSLYFPFRFGASTVLWPEKPDPEKILQVIEKYKPTFFFSVPTLYARFLRIE
                     KKYDLSSLRICLSSGEPLPPAIFQHWQERFGMELLDVVGSTEATHDFLANRPGRAKAG
                     SSGEVTPAFEAKIVDDAGHEVPLGEIGNLMVKGDANSPYYWNKHEQTKRVMQGEWLKT
                     GDTYYKDSEGYFWYCGRSDDMMKVGGLWVSPIEIENSLMEHPAVLEAAVIGDTDADGL
                     LKPKAYVLLKPEFKPSDKLRLELQTHVKTKLAPYKYPRWVEFVEDLPKTVTGKIQRFR
                     LRAGR"
     gene            8626..9330
                     /locus_tag="VF089_16330"
     CDS             8626..9330
                     /locus_tag="VF089_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6175577.1"
                     /translation="MSNMKELKENLALSCNILAYEGHWDNILGHVSVRVPGQERILMK
                     PHSFGFEEIRPEHIIEVDLDGNKVGGKYERHSEVFIHTEIFKARPDVNCVVHSHPPHA
                     VALGSLRQPLHPISHEGNIFYEGLPLFDLTTALIRTPELGVEVAKSLGKCRGLLMKNH
                     GSTVVGESIEVATLYALFLEKAARIQLMATASGNPSWTSDQESAIKYEQIYTPHRLGS
                     MWDYFVRRAKKFRKSS"
     gene            complement(9433..10401)
                     /locus_tag="VF089_16335"
     CDS             complement(9433..10401)
                     /locus_tag="VF089_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175578.1"
                     /translation="MKRCYINLFFVLLTFFLVLPALAQNPVRINWTAVTGAQSGMFMA
                     HLEGLFKKHGVDVELIHIPSSSRGIQAILAGEISFSFMDGVNAAQANLKGANLALVAG
                     ATNRQVFSLMGKPEIKRITDLKGKKIGITRVGSSTHTSALFALASAGLRTNDYQLLPL
                     LEVPNIYTALAAGQIDAGVMSPPTNARAKRSGFVELMNVAKEGPEFVSVAVGTTRSYI
                     KANEEMVRRVVRAYAEGVQLFKSNKPAALKMIQKQLKVSDPEIQEDTYTQFREYLEYP
                     PYVSRKGLEAVLAELADKEAGAKNMKPEDLMDMRFVAELEKKSGGK"
     gene            10506..11471
                     /locus_tag="VF089_16340"
     CDS             10506..11471
                     /locus_tag="VF089_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175579.1"
                     /translation="MSKKVHLTLACGDYESIRALKEGSIKPDGIELTVLTDMTSDIRH
                     WRMVRNREFDVAELSMSNYLIAKYVGLPFCAIPVFLHRRFRHSFIYLNTGKAITKPTD
                     LIGKKVGLRNFQATANLWIRGILEHEYKVPHKQMYWFKQDDEEVEWSPPADLHLERIA
                     SGKHVEQMLVDGELDALIHPELIKPILDKDRRVTRMFPNYRDLEMDYFKRTGIFPIMH
                     TTAIKQEVIDKHPWVAINLMQAFEESKNAAYKRLENPRIVPLAWFRHFLEEQEDVLGK
                     DPWVYGLGETNRKTLETLMQYSQEQGMLGRKMTLEELFINTTAHS"
     gene            11566..11913
                     /locus_tag="VF089_16345"
     CDS             11566..11913
                     /locus_tag="VF089_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175580.1"
                     /translation="MPRGTETSTRVAEEIQLANDKPSLVQTNVSAPKKSWNKSDRNWP
                     DHRTGIQRQWLEGRSAFLALVPENISTASKVGVMGAVYLSQAMIPDMFEGRPGFISLT
                     GATPALRERAGFA"
     gene            12284..12649
                     /locus_tag="VF089_16350"
     CDS             12284..12649
                     /locus_tag="VF089_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175581.1"
                     /translation="MFGKGVHQEEEALLREIIRTIDKKMDYSAREGEGSRFTLHFSAR
                     GRDAVVSLDLEDLKAALRDMTRRNQIRQKIKARYDHLDQSRYGSDILGLKSARLLRAA
                     PKPEPGRQQRGFGRGGPRR"
     gene            12725..>12952
                     /locus_tag="VF089_16355"
     CDS             12725..>12952
                     /locus_tag="VF089_16355"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6175582.1"
                     /translation="MDKAAMIRRSLKRSGQLVMPGVYDALSAQIATKTGFEVIFISGY
                     SLAATMLGEPDFGILTQTEMLSAAQRICSVTA"
BASE COUNT         3174 a         3448 c         3300 g         3030 t
ORIGIN      
        1 ctgctctggt cggcctgcga taaatgccgc cgaatcagat ctgtggcgct ggccttggct
       61 ctttcggcta actcgaccaa cacctggtgc ttggcagcct tgacctcttg gttggcgaga
      121 agctgagcat cgctcttgac cttggcagcc agagcttcgg cttctctgag caagcgcact
      181 ttgtctcgtt cgccttccgc tttcagtgac gcttggatcg agtcaatctc ctggttgagt
      241 gtagctagac ggcttcgata atcctcaact aacgtctccg cgcttcgctt tcgtgccgcc
      301 gcgccggtga ccatggctaa aacttcatca tgcctagact tcaaaaagct acggatcagt
      361 ggaactgcga aataataaat gatcccggca taaataagaa aattcagaaa aggaaaaata
      421 atgccggaga tctcggggga gtgatgctca gcgccaccgg ctgcccaggc ttcagcaacg
      481 aaaagccact cgcgtggatt catcctactc tcctgccgag gatcttactg accatatcat
      541 gcgcaaccgc atcagcctcg cgaatagcca cttcgcgttc ttgcgctaat tggctttgga
      601 cctcttgacg tactcgctcc agaataccag cggcctctcc ccgcgcggaa ctcaaaatca
      661 cctctctctg ctgccgggct tgttgaacaa tcgcttcctt ggtcgcgttt cccgcggctg
      721 ccgcattggc gagggcttcc tcgtattgcg aacgcagccg ctctgcttcg tgttccaaat
      781 ggtaagcact ttcatctgca ccggaggttt tctgttctcg ctcttccaag agcgtcatat
      841 agggccaaaa caggagcttg ctgacgatta gccaaagacc gagaaacaac aaaatctgcc
      901 aaactacaga gaaatctatt gtgatcatga ctaatctgta aacattgcgt ttatctccgg
      961 agccgcggtc cggtttattc caggccaggc ttcaatggag ccagccagag tttgcgacga
     1021 tccgtctatg tctgaacgta aggaaccgga tgggacaaac ggctaatcgt gaggttactt
     1081 actggaatcg ggctgtagtt tcgaggcctg agccaccgca accttttcag tgtttaagct
     1141 tttcgaactc gcgactccag cttgttttgc cgctcccata gagcagtcgc cgtcgaacac
     1201 cacaccctct gtgatgatca gacggggggt cgagacattg ccaaacagcc gacctggcgc
     1261 cagcaactcg actttatctt tggctgttac attgccttct actttcccac ggatgatgac
     1321 gactcgactc gatattctcg cccgcacttc agcaccttca ccgatcgtca atgtctcgag
     1381 acagcggatt tctccttcaa cgcttccgtc gatgcgaacc gacccttcga aagcaagctg
     1441 acccgtaatc cgacttcctt tatgaagctg accgaccagt cgtggttcgg aaggtgtggg
     1501 gacaggttcg gctttcattt taggcccggc caataccagc ggcgcttccg cttccagagt
     1561 tgactgagcc ggcatggaag gtttctccgc ctctgcctct ggcagtttct ttccaccgga
     1621 gtttcgatcg aaccacgcca tattagtgag tcccctattc tgagcaaatt tgctatacca
     1681 tccggacata tcattgtcaa aacaccgcac gtaaaaatga cggctatcct gcgctcccga
     1741 taaccgcccg acatgaggca gtatcttcgg tgttgattgt ttaaactcca aaactgcaag
     1801 tcatcattgc tggcggaaat tccttgacgc taccagatga attgcttagt ttaatagcac
     1861 ttctttaccc cgaagcagcg gctgactgcg acaattttac taattgccaa gactcgcgcg
     1921 agccgtgatt aagtccgaaa ggacaagtgc gatgattcat cgggcgcgca gcaatgcgga
     1981 tatttgaagt tcgcgacgcg taagtgaggt cggaatgtcg aaaatttttc ggatagtcct
     2041 gttgctaatg atcttggcgt cagtggctat agcagcgcag acggcggaca aaaaaacccc
     2101 ggtatacgca acctttaaga ccagcatggg cgacatagtc gttgagctct tcgaagataa
     2161 ggcccccaag acagtggcga attttatcgg tcttgcaacc ggcaccaagg aatggacaga
     2221 tccgaaaacc ggcgagaaag taaagcggcc gctgtacaac ggaacgatct ttcaccgcgt
     2281 gatcccgggg ttcatgattc aagggggtga tccattgggc aacggcaccg gcggccctgg
     2341 ttatcgattc gaggacgagt tccacccaga gcttaggcat aacaaaagcg ggattctttc
     2401 aatggccaat gccggccccc ataccaacgg cagccaattc ttcatcacgc acaaagcgac
     2461 accgtggctt gacggcaagc atactgtctt cggtgaagtg atcaaaggac agaacgtcct
     2521 cgatgcaatc gccaacgttg gacgcgattt tcgcgataaa cctatgaagg agattgtgat
     2581 tcagcaagta aacatcagcc gcggttctta ctaagctgta aaccgcgcaa gacaagagcg
     2641 ctactcaaga tcatacgtaa tctcgagttc ttcgctcaga tcacggagac cccgaagaac
     2701 agagccgagc aaaggaatac cttcctcggc tctgcgcttt gctgtttcga attctatttc
     2761 gccagcgaca tagactcgct cttgaccttc tatgggccga gtcgacttca attcggtgat
     2821 tagtcggtcc atgtcgctct tgaattcgct aacagggcga aatgcatcga tccttatggc
     2881 cacgaagaag tggcctcgcg gatcctgatt ttggtttagc gcggtcaccg tgccggtcag
     2941 aacgccacat agaatctcaa ctaagatcgc caagccgtac cctttgtgac tgccgccttc
     3001 tcgagtgcca cccaaaggca atagccgccg agccttctgc gcaatcctgg gatcgctggt
     3061 aggctcagct ttctcgtcta gggcccaccc tagcggaatc gacgcgccgc gacgcgctgc
     3121 gacttccagt tttccggcag cagcggtggt cgtcgccatg tcaatgacaa aaggaagctc
     3181 cttttgtgca ggcgcagcaa aacagatcgg gttggttccg aatcttgctt cgctgccgaa
     3241 agttggaacc acttggcgcc ctgcatttgt cattgcgata ccaatcatgt cgtgcggcaa
     3301 ggcttgcatc gcataatatg cgcacatgcc aaagtgccgg ctattgcaaa ctcctacgat
     3361 accgacgccc gaaaccttgg ctttttcaat tgctaattcc attgcgcggt ggccgaccac
     3421 catgccaatt cctcggtcac cgtctagtaa agcggatgat ccagcgtcgg agacgatacg
     3481 gatctttggt tccggagtca tttcgccgtc gcgcaaccat ttcacatacc agcttcctgg
     3541 attaaaccgg atgacaccat gggtatccac ccctcgtaga tcagccatca caagaacatt
     3601 cgcggcgatt gcggcgtcct gtcgcggcac accgagcttt ttgaaagcag cagccacaaa
     3661 gcgtgttagt tttcgatgat cgacgagagt agtctgttgc ggattagtta tcataatgac
     3721 ctgaacgttc ctttgtctcg gtcagctcac cttatgtagg aaatctttat cagcgagatg
     3781 atcgccggaa agtctcctgg cctcacgcac ggacgaggca cgcatttttg gaattctcgc
     3841 gatgcccatc aaagccacga taatcttcaa acgaagccag aaacaacggc acccgatgat
     3901 ccagcccgtg gcaaaccaac atccaataaa cgacattaag gccagaacgc cgaactttgc
     3961 gcaacggtgc gccgtcgcgc gaaggccgca agagtatcct ccacctggcg ctccgccgcg
     4021 ggcgcacccg cctggtctcc ggccgcttga agtacatagc gacggtccag gattcagcca
     4081 aatcctagct ggcaaaccga cccagggggc tgcctgcgcg tcagttcatg cctcgcgagt
     4141 tcaccggcaa ttcggactaa caatcaacat ttgagtgctc atccaaagta aaatcagacg
     4201 agtatctgca agaaaaatcc tttgagctga gcgcgcccgc actgctcgaa cggagattga
     4261 cgacagcgat ttcgcctaac tcgaacgttt agcttgcgcc tttgctgtgc gatttcagca
     4321 gctcggcgaa ctgtttgtgg agcttgttca atttcggcgg tatcatcact tgacaatagg
     4381 gttggtacgg attgtttgcg taatacttct gatgatagtc ctcagcttca tagaagtctg
     4441 tgagcgcttc caaagtagta acaatcggtt tgctatagct gccggcggct tctagctctt
     4501 taataaagtt ttccgcctca cgtttctgct catcgtcggc ataaaatatt gccgagcgat
     4561 actgcgtacc aacatcgttg ccctgacgat taagcgtcgt cggatcatga gtggcaaaga
     4621 aaaccgttag caagtcccgg aaagcgatct gatcgggatc gaattcaatg cgaatcactt
     4681 ccgcatgtcc ggtgcggcca ccgcagacct gctcataggt gggatttttc aactccccac
     4741 cggcatagcc cgaaacgact gaagtgacgc cacgaagttc gtcgaatacc gcttcggtgc
     4801 accaaaaaca tccgccacca aaaacagcga ccccgttatt ctgtgtcatc gtgccctcaa
     4861 gttctggttc agttcgctca gtatgaaaat tgtcaactct tgtgcagcga cggcgccgca
     4921 gggccaacgg tccgtgcgtt ccgtttgaga tattcagtta tcccctcttg gccgagcttc
     4981 cataagcgcc ggtgaatgcc gaggcgataa accataggtt tcaagcagtc tgaaaaatca
     5041 acgtttaaaa agttggctaa gacttcaccg ctcgggctct cattctactg aacgtaacaa
     5101 ttcgcctgcg cgtggaagcg aactccgatt taccagattg cgttctcctt aggtctggca
     5161 acggacggca ctatcaacgc ccgagcagaa agagcagcag tacgagcttc acagtcaatt
     5221 gagatacgat ctttcaagca aagtcttatc aatgccattc gatcaaatca atccattccg
     5281 catcactcaa ctggtccgaa gttacgacat ttccgcttgt cgcactatgc cagatgtcta
     5341 gttggacaaa ctcgcgtaat cagccgccaa ggctagagcg ttggccgctg cggctaacgg
     5401 attagacggg aactacctag gcagctacag tcgtaagttg atccgctaat cttgacaacc
     5461 cgctagctac atgattgttt ttgaagttcc tagtcacgga ggagtttatg gcaaaactgc
     5521 ttatcgccag tgagcaggtg gactcggaaa gtaacgagac caccgcagtc aaaaatccag
     5581 ccaccggaga gcttgtcgat tcggttccca aaggaacggt aaacgacgtc cgtcgcgcca
     5641 tcgatgccgc caaaggagcg ctgaaaaaat ggtccgagat ggctccgtcc aagcggggtg
     5701 cgattttact tcaagccggc catgcgatcc tgcaacaaga aaaggagatt gcgactttat
     5761 tgactcgcga gcagggcaaa ccgatgcggg aatccatttt ggagatccgc cgttttgtgc
     5821 acactctcga tcattacggc ggaatggcaa agagtctccg taatgctgcg gttatgcttg
     5881 acgtcggtcg ccacggttta gtgttgcgca agcctttggg cgtctgcggc tccatcgtac
     5941 catggaattt tcctgtttct ttgatgggca ataagctcgg tccagcctta ttgatgggta
     6001 ataccgttgt cgttaaaccg gcgggcacga cgccgctcac cgatttgcgc tgttgcgaaa
     6061 tgatcgacag agccatccaa gatgctggcg gtcctcgtgg agttctcaat gtcgtcaccg
     6121 gtcccggcag cgttgttggt gaggaattac tcgtcaaccc gacagtacgc aagatcggtt
     6181 ttaccggggc caccgatacc ggtcgacgag tgatgcagtc ggcggccaaa gacttcaagc
     6241 acgtcacctt ggagctgggt ggcagcgatc cgatgatcgt ctgcgacgat gctgacatcg
     6301 atcgcgccgt cagcgccgct tctgtcggtc gcttcttcaa ttgtggccaa gcctgtctgg
     6361 cggtcaaacg gctttatctg ttcgaccgta tcgccgatag tttcatggat aagttggttg
     6421 agaaagttaa aaggatcagc gtcggcaacg gcctgaaggc aggagtcctt atggggccgc
     6481 tacacacggc cgaacagcgg cgggaagtcg aagaacaagt tgaagacgca gtgaagcgcg
     6541 gcgccaaggt gctctacgga gcgcaacgac cgaaaggcga tgactacgag aaaggatttt
     6601 atctgcagcc caccttggtc gccgatgtcg acccggcatc tcggctgctg caggaagaat
     6661 gctttggtcc agccttgccc attgttcggg ttaaaaatct ggatcaagcc atcgagcaag
     6721 ccaacaactc gatctttggc ttgggttcat ctatttggac gcgtgacatc aatcgtgcga
     6781 tggcgggcgc ggaacgcatc gaagccggat acacttgggt caattcggcg cagatcatct
     6841 atgacgagct accgttcggg ggcgtcaaac agagcggttt gggcaaggaa catggcgagg
     6901 aggcgatcga gcactacacc gactccaagt cagtcgttat tgctactgag acgcattcgg
     6961 agatggtcgg cggcgaatga aacgcctgcg gcaatcgaac gagcgggctc tcaattagct
     7021 tgaggtttcg accggagcaa ggatgggagt cgaactgcct gagatctata atgccgccac
     7081 gacttttgtg gatgaaaaca tcactcaagg tcgcggcggc agagtcgcaa tttactacca
     7141 agacgaggcc atcacctacc aagaagtcta cgagaaagta aaccggaccg gcaacgcttt
     7201 aaaggagctc ggtgtcgaaa tcgagcaccg ggtattactt gcccttccgg actcgcctga
     7261 atttgcttac agcttttttg gtgccatcaa aatcggcgcc gtgccaattc cgacgaatcc
     7321 ttggatggcg gcaaaagatt acgaatacct gatcaatgac agtagagcgc gtgcgatcat
     7381 cgtgcatgaa tccgtgcttc ctgaagttgc aaagatctgg aacaacacac gatatcttaa
     7441 acagattctg gttgtcggaa aaggcaagga cagagcgcga tcctaccaag agctcattgc
     7501 tcgagcatcg gattacctgg aaccagaacc aacccactgc gatgatgtgg gcttttgggg
     7561 ttacacgtca ggcagcactg gaatgcctaa aggggcggtt cacctgcagc acgacatgat
     7621 aacgatcacc gatctgttcg tgaaaccaat cttgggcatg aatgaaaacg atcgctgttt
     7681 ttcggcatcg aagctttttt tctcttacgg gttgggcaac tcgctttact ttccctttcg
     7741 cttcggcgcc tcgaccgttc tctggccaga aaaaccggat ccggagaaaa ttcttcaagt
     7801 catcgaaaaa tataaaccga cgttcttctt ttccgtgccc acgttatatg cacggttctt
     7861 acgaatcgaa aagaaatacg acttaagctc gctgcgcata tgtctttctt ccggcgagcc
     7921 attgccgccg gcaatatttc aacattggca agagcggttc ggcatggaat tgcttgacgt
     7981 cgtcggttcc accgaggcaa cccatgactt tctcgccaat cgcccgggca gggctaaggc
     8041 cggcagtagc ggcgaagtga cccccgcttt tgaagccaaa atcgtcgatg atgccgggca
     8101 cgaagttccg ctgggggaaa ttggcaatct aatggtaaaa ggcgatgcca actctcccta
     8161 ctactggaac aaacatgagc agacaaagcg agttatgcaa ggcgagtggt tgaaaaccgg
     8221 tgatacgtac tataaggact ctgagggtta cttttggtac tgtggccgct ccgatgacat
     8281 gatgaaagtg ggcggtttat gggtctcccc aatcgagatt gaaaacagct tgatggagca
     8341 tccggctgtg cttgaggccg ctgttatcgg ggacaccgat gccgacggtt tgctcaagcc
     8401 gaaagcgtat gtcctgttga agcccgagtt taaacctagc gacaaacttc gtttagaact
     8461 ccagacacac gtcaaaacca agcttgcgcc ttacaaatac ccgcgctggg tcgagtttgt
     8521 cgaggacctt cctaaaaccg tcaccggcaa aatacaacgc ttccgcctgc gcgccggcag
     8581 gtaagcttag aagtagctgc aacttactag tgaggggaca caactatgag caacatgaaa
     8641 gaacttaaag aaaacttagc attgtcgtgc aacattctcg cgtacgaagg tcactgggac
     8701 aatattcttg gccacgtgtc agtcagagtt cccggccaag aacgaattct catgaagccg
     8761 cacagcttcg gctttgagga aatacgaccg gaacacatta tcgaagtcga tttggatggc
     8821 aacaaagtgg gcggtaagta cgagcgtcat tccgaggtat ttatccacac tgaaattttt
     8881 aaggcacgac cggacgtaaa ttgtgttgtg cactcccatc caccgcatgc agtagcgctc
     8941 ggttcactgc gtcaaccgct tcatccgatc agccatgagg ggaacatatt ttacgaaggg
     9001 ctgccgctct tcgatctcac aaccgccctg attcgaactc cggaactcgg agtggaagta
     9061 gccaagagct tggggaaatg tcgcggccta ttgatgaaga atcatggatc caccgtcgtc
     9121 ggggaaagta ttgaagtggc cacgctctat gcgcttttcc ttgagaaagc ggcacgtatc
     9181 cagcttatgg cgacagcttc gggcaatcca tcgtggacca gcgaccaaga atcagcgatc
     9241 aagtacgagc agatctacac accgcaccgt ctgggttcca tgtgggatta ttttgtgcgt
     9301 cgcgccaaga agtttcgcaa atccagctag catcgctgcg cgaactctga gccgcgatgc
     9361 cggatccgga cctgtagggg gccaagcgtt cgagatcaca ggtaagcgcc ggaccttgca
     9421 acgttagaat cgctactttc caccggattt tttttccagc tcggcaacaa agcgcatgtc
     9481 cataaggtct tccggcttca tgttcttagc tcctgcctct ttgtcagcca gttcagccaa
     9541 caccgcttcc aggcccttgc ggcttacgta aggtggatat tccaaatatt cacgaaattg
     9601 agtgtacgtg tcttcctgga tttcaggatc acttacctta agctgctttt gaatcatttt
     9661 cagtgccgcc ggcttgttac ttttgaatag ctgaacacct tcagcgtatg cccgcaccac
     9721 tcgtcgaacc atctcttcgt tggccttgat ataagaacgg gtggtcccaa ccgccactga
     9781 aacgaactcg ggaccttctt tggcgacatt cattagctcg acgaatccgg accgcttcgc
     9841 tcgcgcatta gtcggcggtg acatgactcc ggcgtcaatt tggccggcgg cgagcgcggt
     9901 atagatattc ggtacttcaa gcagcggcaa caactgataa tcgttcgttc tcaagcccgc
     9961 cgaagcgagc gcgaaaagcg ccgatgtatg cgtggatgag cccactcgcg tgatgccaat
    10021 cttttttcct ttcaaatcag tgatacgctt tatttccggt ttgcccatga gcgaaaacac
    10081 ctgccggttg gtcgctcccg ccaccagggc caggttcgct ccttttagat tggcctgcgc
    10141 cgcgttgacg ccgtccatga atgagaaaga tatttcacct gccaatatcg cctggatgcc
    10201 acgcgagctc gaaggaatat gaatcaactc aacgtcaact ccatgttttt tgaacaatcc
    10261 ttccaggtgc gccatgaaca tgccgctttg cgccccggtt acagccgtcc agttgattcg
    10321 gacaggattc tgggcaagcg ccggtaaaac cagaaaaaac gtcagaagca caaaaaacaa
    10381 gtttatatag catcgcttca tctacaaacc cccatttatc ggcccatgcg taataccaaa
    10441 gccctatagg agagtcaaat ccggaattcg ttggcaatct cgttgaaccg tgctagatag
    10501 gccgcatgag caagaaagtg cacctgacgc tggcttgcgg tgactacgag agcatccgcg
    10561 cactcaagga aggaagcatt aagccggacg gaattgaact gacggtatta accgacatga
    10621 cttcggacat ccgccactgg cgcatggtcc gcaatcgcga gttcgatgtc gccgaactgt
    10681 cgatgtcgaa ttatctcata gccaagtatg ttggcctgcc gttttgtgcc ataccggtct
    10741 ttcttcaccg tcgcttccgc catagcttca tctatcttaa tactggcaaa gctattacca
    10801 agccgacgga cttgatcggc aagaaagtag ggttgcgtaa ttttcaagcc acagcaaatt
    10861 tatggattcg tggcattttg gagcacgagt acaaagtgcc gcataagcag atgtactggt
    10921 tcaagcaaga tgatgaagaa gtggaatgga gcccgcccgc ggatttgcac cttgaacgga
    10981 tcgcttcagg aaaacatgtt gaacaaatgc ttgtcgatgg tgagttagac gctttgattc
    11041 atcctgagtt gattaaaccg attttggaca aagaccggcg agttacccgg atgtttccaa
    11101 attaccgtga cctcgagatg gattatttca agcgcacggg catttttccg attatgcaca
    11161 ctaccgccat caagcaagaa gtcattgata agcatccgtg ggtggcgatc aacttgatgc
    11221 aggctttcga ggaatccaaa aacgccgcat acaagcgatt ggaaaacccg agaattgtcc
    11281 cattagcatg gtttcgtcat ttcttggaag aacaagagga tgttctcggc aaagatccat
    11341 gggtttacgg actgggggag accaatcgca agactttgga aacattgatg caatattctc
    11401 aggagcaagg catgctcggt cgtaaaatga cgcttgagga attgttcatc aataccaccg
    11461 cccattcgta agatcttgtg gaaaccggcc atcatcgccg tgtcgcaacg ggattaagtt
    11521 catccttagc gcgcgattgt gcggcaaagt tatgccgtag cacttttgcc tcgcggcact
    11581 gagacaagca cccgggttgc tgaagaaatc caacttgcga atgacaaacc gtcattggtc
    11641 caaaccaacg tttccgcgcc gaagaagtcg tggaacaagt ccgatcgaaa ctggccagat
    11701 caccgcactg gcatacaacg ccagtggtta gagggtcgca gcgcgttctt agcactcgtc
    11761 cctgaaaata tctcaacagc atccaaggtc ggcgtcatgg gcgcggtgta tctgagtcaa
    11821 gcaatgattc cggatatgtt tgaaggccgg cctggcttta tatcactgac tggagctacc
    11881 ccggcgctgc gcgagcgtgc cggctttgca taactggcaa tcgccaaggc caagtgtaga
    11941 tgttagttca atcattggcc cgcgtttcgc ctaaaaattc atgttgtttg aattgatcgt
    12001 agacggatca atcgatactc ccaaactgcg ggccggcgat ccaaaccggg ctgctgaaac
    12061 catctttaca cctgattgat gccgaaaccg aacaaacgcc agatacgtgg actcacctgg
    12121 aattattctg aaacctgacg caggcgcctt cgcctgatgt ttgatcttac cggcacttta
    12181 gcgtagattt tcacaacctg gcggtacttg gcaagatgga tgttcatcat cccattgggt
    12241 taagtagctc aggaatcgtc tatttgccag ggaggaaatc atcatgtttg gaaaaggcgt
    12301 tcatcaagaa gaagaagcgt tgctccgaga aatcattcgc acgatcgata aaaaaatgga
    12361 ttacagcgcc cgtgaaggcg aaggatcacg ctttaccctg cattttagcg cgcgtggtcg
    12421 tgacgccgtt gtctcgctcg atctcgaaga tctcaaagct gctcttcgag atatgaccag
    12481 gcgcaatcaa atccgtcaga aaatcaaagc acgctatgat cacctggatc aatcgcgcta
    12541 cggctctgac attttaggct taaaatcggc cagactgttg cgcgccgcgc ctaaaccaga
    12601 gcctggaaga cagcagcggg gttttggccg cggcggaccg aggcgctgag ttaacttccc
    12661 accgattagt tcaccgaagc acgtgcccga tcggctgttc ataaatccgg cttaggagtt
    12721 tcctatggac aaagcggcaa tgattcggcg atcattgaaa agaagcggcc agttggtcat
    12781 gcctggggtc tacgatgcgc tgagcgcaca aatcgcaact aagacaggtt tcgaagtcat
    12841 ctttataagc ggttatagcc ttgctgcaac gatgctcggg gagcctgatt tcggtattct
    12901 aacccaaacg gaaatgttgt cggcagctca gcgaatttgt tcggtgaccg ct
//
LOCUS       DASUTG010000707         7506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_110234, whole genome shotgun sequence.
ACCESSION   DASUTG010000707 DASUTG010000000
VERSION     DASUTG010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110234"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF089_16360"
     CDS             <1..386
                     /locus_tag="VF089_16360"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175583.1"
                     /translation="EVRGVNMTPGGTFRHHVVVSIKKRAENEGRNVILALLSMGIGLK
                     QVIVVDDDIDPFDPMQVDWAMATRFQADKDTIIIPRIACSTLDPSCPEPRVTAGLGID
                     ATAPMKDRWRFEKVEIPGVDKVKYI"
     gene            506..1375
                     /locus_tag="VF089_16365"
     CDS             506..1375
                     /locus_tag="VF089_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6175584.1"
                     /translation="MKNFDYLEPRTVAEACAMLKQHAGEAKVFAGGAHVTILMKQGLY
                     QPKALVNIKKISELKNISFDTTEGLTIGALVTHREIETSSLVHEKLPMLSEAEREIAN
                     IRVRNMGTVGGNLASGEPLTDLPQIFIALDGKLKIAGPSGQRTIRVEELFLDFYTTSL
                     AEDEIIIQVVLPPPPPRSGVDYIRFSSSSVVDKPSAGVAVRLTLDEKAVQVARIVLGC
                     VGPTPIRAKKAEALVTGKILNDELIGEAGALASQECSPIGDLRGSEEYKRAIVRTLVK
                     RAAGAAYKQALHG"
     gene            1581..2051
                     /locus_tag="VF089_16370"
     CDS             1581..2051
                     /locus_tag="VF089_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6175585.1"
                     /translation="MKRKTVTLKVNGVERSVEVEARTTLLDLVREQFGLTGAKLGCDI
                     QVCGACTLLLDGKPVSACSVLAVDADGYKVVTVEGLGSDGKLDPIQEAFMEFGALQCG
                     YCTSGFVLTAKALLEECPRPSDEQIRNYLAGNFCRCGCYQEIIQAVKNVSRALD"
     gene            2095..4419
                     /locus_tag="VF089_16375"
     CDS             2095..4419
                     /locus_tag="VF089_16375"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6175586.1"
                     /translation="MSTGEIGKSARRLDYESKVSGRAMYLADMHIPGMCHGKILRSPL
                     PHARIKGIDTAKALKVAGLITIITRDDILHDQGIEPYYGPVFKDQTIVAVDKVRHVGD
                     PVAAVAATTVDAAEEALRLIEVDYEELPAVLDVHEALKGGSVLVHDAIKLPSSGFADL
                     AEIKPVEGTNICTHFRLNRGDVENGFIEADRIFEDTFTLPATQHSFLETHACIASVEA
                     GRITVWATTQNPFVVRTQLANIFKIPVSKVRVIVPYLGGGYGGKVYPKIEPITVALAQ
                     KARRPVRVVLSREEVFYTITKHAAVIRMKTGVKNDGTLVARECEIHLDTGAYAEIGPR
                     VAKKSGYTAAGPYRIPNLKIDSYSVYTNKPPAGAFRGFGVSQSAWAVESQMDIIAAAL
                     KMDPLELRLKNGYVDGDRFVTEETLRSVGLKDCLDQVAKSIGWEARTFKNSNVQRFND
                     GNLKRGKGLACMIKATITPSISCAAVKLNEDSSLSIYCGTVEMGQGCETVLAQIAGEE
                     LGIPLRQIQVLGVDTDVVPYDLTTSSSRSTFHMGKAVQLAARDIMRQLKEIVVKEYGV
                     PADQVSFADGKVRLPEGSLDYAEIMFKRFGMQGGTLLGEGELKTSVKDEYGEKSTSAF
                     WFLAAGAAEVEVDVETGKFKLLKYATAVDVGKALNPLGCRQQLTGAAITGIGQAVFEE
                     IAYDNGQLINPNLIDYVLPSLADMPASIDPIAIEIPDRNGPFGAKGIGESALIPVAPA
                     IANAIFDAVGVRIKDLPIKAEKVYLALEDSKNKT"
     gene            4443..4880
                     /locus_tag="VF089_16380"
     CDS             4443..4880
                     /locus_tag="VF089_16380"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6175587.1"
                     /translation="MSRISGVISLAIFTVAMFACNSEMLQKQAEEIKQQELEIARQRK
                     DLEAMLASQKLQDQKQQDCNRAFRDYFEKAQIASAGDEAIELYREGLSLCPEDEVARY
                     ELGRALADRGRYVEAEKELETALSLRPDFTDARRQLESVRKNR"
     gene            5104..5793
                     /locus_tag="VF089_16385"
     CDS             5104..5793
                     /locus_tag="VF089_16385"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6175588.1"
                     /translation="MTYPFAEDTLHWPTAKPFHLEKTSEGRTAAGYWYSAYEYSSAEH
                     VGTHLDAPFHFAQGKWTTDQIPLARTIGRGVVIDVRGAVEADRDYRLSVQDIHDWEKR
                     FGRIPKESIVLMYSGWGNHWGNRKQYFGTDEPGDVVNLHFPGFSKEAAEFLIKQRAVK
                     AIGVDTPSIDHGPSRDFIVHQILGGANVPIFENVAALDRLPAKGATIFAIPMKIKGGS
                     GAPLRIFAQLP"
     gene            5906..7294
                     /locus_tag="VF089_16390"
     CDS             5906..7294
                     /locus_tag="VF089_16390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6175589.1"
                     /translation="MSVWKSTLFPCLLVYVWMGTVRPMSVSLAADVALQVQATPATQS
                     PPQGKLLTIEDAVRIGLENHPRIRAARERIGSQEAILGQQMSAYYPTINFNNSYRTTN
                     SSGTSGTNSSATDTYTSQANLNLTLYNFGKREGTVQAARETVDATREDFSTTTQDIVL
                     SVKQAYYTYLGTQALVTVRQETVRNRELLVRQAQGFYEVGTRARIDVARAEANLYTAR
                     ADLIASENSVKIAWVTLRNAMGSPRLPEQPVAQDSPEVELSMNLAAARTIAFDNRTEL
                     RSFEAQRRASDQLIATARRGHLPDIVFDAFYGRRNSSGGGESTFPLQPAWQAQVSLNI
                     PIFDGFRTTNRVEETLHNYYNIRAQEEERRQQIALEVEQSYLRVVETQERIIATESAT
                     RAAKENLDLAQGRYQVGVGSIIEVTDAETLYTDAQTNYVRNLYDYRIADAQLARAMGD
                     HRVGVLKPGSIR"
     gene            7327..>7506
                     /locus_tag="VF089_16395"
     CDS             7327..>7506
                     /locus_tag="VF089_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175590.1"
                     /translation="MKRTLLLVILLVVIGAFAGFQQYSSWSLNRSQNNLEVKPQEGAV
                     QASRGQSRPGNQGRGG"
BASE COUNT         1797 a         1943 c         2141 g         1625 t
ORIGIN      
        1 ccgaagtacg cggcgttaac atgaccccgg gagggacctt ccgccatcat gtcgtggtgt
       61 ccattaaaaa gcgcgccgag aacgaagggc gcaatgttat tctggcgctt ctttcaatgg
      121 gcatcggtct caagcaggtg atcgttgttg acgacgatat cgatcccttt gacccgatgc
      181 aggtagattg ggcgatggca acacggttcc aagcggacaa agacacgatc atcatcccac
      241 gcatcgcttg ctcgacgctc gacccatctt gtccggaacc tcgtgtcacc gccggcctag
      301 gtatcgatgc taccgcgccg atgaaagatc gctggcgctt tgaaaaagtc gagatacccg
      361 gcgtggacaa ggtgaagtac atctaagatt tggaagaaca ttactcgcgc aaaggcgcaa
      421 aggacgccaa ggtcggaatt agaaacgccc cttaacttgg cgcctttgcg tcttggcgcg
      481 atagttctta aatgctgcac aacagatgaa gaatttcgac tatctcgaac ccaggaccgt
      541 agccgaggcc tgcgccatgc tcaagcagca tgccggtgaa gccaaagttt ttgccggcgg
      601 cgcgcatgtc acgattctta tgaagcaagg cctgtatcag cccaaggcgc tggtcaatat
      661 caagaagatt tctgagctaa agaacatttc gtttgacacg accgaagggt tgaccattgg
      721 cgctctggtg acgcatcgtg agatcgaaac ttcctcactc gtccacgaaa aacttccgat
      781 gctctccgaa gccgaaagag aaatcgcaaa tattcgggtg cgcaatatgg gaaccgtcgg
      841 gggcaatctc gcttccggtg aaccgttgac cgatctacca cagattttta ttgcgctaga
      901 cggcaagtta aaaatcgccg gcccatccgg gcagcgcacg attcgggtcg aggagttatt
      961 cctcgatttt tatacgacga gtttggcgga agatgaaatc atcatacaag tggtgcttcc
     1021 gccgccaccg ccgcgctccg gtgtcgacta cattcgattt tccagcagct ccgtagtcga
     1081 caaaccttca gccggtgtcg cagtgcgact gaccttggat gaaaaagccg tccaagtcgc
     1141 caggattgtg ctcggctgtg tcggtccgac ccctatcaga gcaaaaaagg ccgaggcgct
     1201 tgtcaccggc aaaatattga acgacgagct cataggggag gcaggcgccc tggcgtcgca
     1261 agagtgcagt ccgataggcg atctgcgcgg ttccgaggaa tacaaacgag ccatcgtcag
     1321 gacgctggtg aaacgggcgg caggcgcggc ttacaaacaa gcactccacg gttaatcgcg
     1381 aaaggaaaaa ttggaccctt gtaatacgtc tttgaattgt gcgacgcatt gtcctttccc
     1441 ctcaatgtgg gaaagtaagg atgggtgtct tgagcagccg cactctaagt ctcccccgtc
     1501 gagggggaga gcatccggag tcgctgcgga agatacgcta tgctcaatcc tcggatgttt
     1561 tgcagcgcag tagtaggaaa atgaaacgga aaacggtcac actcaaagtc aacggtgtcg
     1621 agcgtagtgt cgaagtcgaa gcgcgcacca cgctgctcga tttggtgcgc gagcaattcg
     1681 gtttgaccgg cgccaagctc ggctgcgata tccaggtgtg cggcgcttgc acgttgttgc
     1741 tcgacggcaa accggtaagc gcgtgttctg ttttggctgt cgacgcggac ggttacaagg
     1801 tcgtcaccgt cgaaggattg ggcagcgatg gtaagctcga tccgatccaa gaagccttta
     1861 tggagtttgg cgctctgcag tgcggctatt gtacctcggg attcgtccta actgcgaaag
     1921 ccttgctgga ggaatgtcct aggccatccg atgagcaaat tcgaaattat ttggccggca
     1981 acttttgccg ctgcggctgt tatcaggaga ttatccaagc ggtgaagaac gtctccaggg
     2041 cgcttgattg agactaacct tcgggaaaat gcgaagaacg atgtgggatc ttcaatgagc
     2101 acaggcgaaa tcggcaaatc cgcgcgacgg ctggattatg agagcaaggt ctcgggcagg
     2161 gcgatgtatc tagcggacat gcacatcccg gggatgtgcc acggcaagat actgcgcagc
     2221 ccgctgcctc acgcccgcat aaaaggcatc gacactgcaa aagcgctcaa agttgccggc
     2281 cttatcacga tcattacccg cgatgatatt cttcatgacc agggtattga accttattac
     2341 ggtccggttt tcaaagatca aaccatcgtg gctgtggata aagttcgcca tgtcggcgat
     2401 ccggtggcag ccgtcgccgc gaccaccgtt gatgccgccg aagaggcgct gcggctgatt
     2461 gaagtcgatt acgaagaact gccggcggtg ctcgatgtgc atgaagcgtt aaaaggcggc
     2521 agtgtattag tgcacgatgc aatcaagctg ccgagttccg gtttcgccga cctcgccgaa
     2581 atcaagccgg tggaaggaac caatatctgc acgcacttca ggctcaaccg cggcgacgtc
     2641 gagaatggtt tcatcgaagc ggaccgcatt tttgaagaca cctttacctt accggcgacc
     2701 caacacagtt ttctcgagac tcatgcctgt atcgcgtcag tggaggcggg ccgcatcacg
     2761 gtttgggcga cgacgcagaa tccattcgtc gtacgcactc agttggccaa catattcaaa
     2821 atcccggtgt cgaaggtgcg ggtgattgtg ccgtatctcg gcggcggcta cggcggaaaa
     2881 gtttatccca aaatcgagcc gattacggtt gccctggcgc aaaaggcccg ccgtccggtt
     2941 cgggtggtgc tgtcgcgtga ggaggtcttt tacacgatca cgaaacacgc cgcggtgatc
     3001 aggatgaaaa ccggcgtgaa aaatgacggc accctggttg cgcgagaatg tgagatccat
     3061 ctcgacaccg gtgcttatgc cgaaattgga ccgcgggtgg cgaagaaatc aggctacaca
     3121 gcggcggggc cgtatcgaat tcccaacctg aaaatagatt cctattccgt ttacacgaat
     3181 aaaccaccag cgggggcttt ccgcggcttc ggggtttctc aatcggcgtg ggcggtcgag
     3241 tcgcaaatgg atatcatagc agcggctctg aaaatggacc cgctcgagct tcggctcaag
     3301 aacggctatg tcgatggcga tcgattcgtg accgaagaga cattgcgcag cgtcggcttg
     3361 aaagattgcc tcgaccaagt ggctaaatcg attggatggg aagctagaac tttcaaaaat
     3421 tcaaacgttc aaaggttcaa cgacggaaac ctgaagcgcg gcaaggggtt ggcctgtatg
     3481 atcaaagcga cgatcacgcc atcgatatcg tgcgccgcgg tgaagctcaa cgaagacagc
     3541 agcctgtcca tctattgtgg tacggtcgaa atgggccagg gttgcgagac agtgctggcg
     3601 cagatcgccg gagaagaatt gggaattccg ctgcgccaga ttcaagttct cggcgtcgac
     3661 accgatgttg tcccttatga tttgacgacg tcatcaagcc ggtcaacctt ccacatgggc
     3721 aaagcggtgc aactcgcggc gcgcgacatc atgcggcagc taaaggagat agtcgttaag
     3781 gagtatggcg tgccggcgga ccaggtgagc ttcgccgatg gcaaggtgcg cttgcccgag
     3841 ggttctctcg attatgctga aatcatgttc aaaagattcg gcatgcaagg cggcacttta
     3901 ctaggagagg gcgagttaaa gacatcggtg aaagacgagt acggcgaaaa gagcacctcg
     3961 gcattttggt tcctggcggc cggcgctgcc gaagtcgaag tggatgtgga gaccggcaaa
     4021 ttcaagctgc tcaagtatgc aaccgcggtg gatgtcggta aagcgcttaa cccccttggc
     4081 tgtcgccagc aattaactgg cgcggcaatt accgggatag gccaggcggt attcgaagag
     4141 atcgcatacg ataatgggca gctgatcaat cccaatctta ttgattatgt gttgccgtct
     4201 ctggctgata tgcctgcttc gatcgatccg atcgcgattg agattcccga caggaatggt
     4261 ccattcggcg ctaaaggcat cggcgagagc gcgttgatcc ctgtggcgcc ggccatcgcc
     4321 aacgcgatct tcgatgcggt cggcgtgcgc atcaaggatt taccgatcaa ggctgaaaaa
     4381 gtttatctgg ccttagagga ttcgaaaaac aagacgtagc tcacggctgc gcaagcttta
     4441 acatgtctcg catatcaggc gtgatttcat tggctatttt cacggtagcg atgtttgcct
     4501 gtaattccga gatgctgcaa aaacaagccg aggagattaa gcagcaagag ttggaaattg
     4561 cccgacagcg taaggatctc gaggcaatgc tggcaagtca aaagctgcaa gatcagaagc
     4621 agcaggattg taaccgcgct ttccgcgatt actttgagaa ggcccagata gcaagcgctg
     4681 gcgatgaggc gatcgagctt tatcgcgaag ggctgtctct ttgcccggag gatgaggtgg
     4741 cgcgttatga gctcggccgg gctttagcgg atcgcgggcg ctatgttgag gctgagaagg
     4801 agctggagac ggcgctcagt ctcaggccag actttaccga tgctaggcga caactcgagt
     4861 ccgtccgtaa aaaccggtag cccaaagctc aagatagtga ccgggtagca agttttgccg
     4921 ccggattctg tttgtgacac tgaaaaacag cctggtttct ctccgtgctg ctttggctac
     4981 ctagatcagt ctatctgaac cttgctaggg ggtagaggga tgcgagccgt cctcggcttt
     5041 ttattttgct tagttgtatt tgcctcttcg gcgcacgcaa tcgatgctac gaagctaatt
     5101 gatatgacct acccgtttgc cgaagacacg ctgcattggc cgactgctaa gcctttccat
     5161 cttgagaaga ccagcgaagg acgaaccgcg gcgggctact ggtattccgc ctatgagtac
     5221 agcagcgccg agcacgttgg gacacacctg gatgcgccgt ttcactttgc ccaaggcaaa
     5281 tggactacag accagattcc attggccaga acaatcggtc gcggtgtcgt tatcgacgtg
     5341 cgcggcgcgg tcgaggccga tcgcgattac cgattaagcg tccaagacat tcatgattgg
     5401 gagaagcgtt tcgggcggat tcccaaggaa tctattgttc tgatgtactc gggatggggt
     5461 aatcattggg gtaatcgcaa gcagtacttc ggcacagatg aacccggcga cgtagtcaat
     5521 cttcacttcc caggattttc gaaagaagcg gcggaatttc ttataaaaca gcgcgccgtc
     5581 aaagccattg gcgttgatac cccgagcatc gaccacggcc catcgcgcga tttcatcgtt
     5641 catcaaatac ttggcggcgc caatgtgccg atcttcgaga atgttgcggc attggatcgt
     5701 ttgccggcaa agggcgcaac catcttcgca atcccgatga agatcaaagg cggaagcggc
     5761 gctcccctcc gaatcttcgc ccaacttccg taggactttc gcgaatttcc cgcctgaaag
     5821 agtcacgatt gttccgtccg cgctttttgc tttcacctgt taaaaccact atagtctgcc
     5881 cgcacaaagt taagggagga agagtatgag cgtgtggaaa tcgactctgt tcccatgtct
     5941 cttggtctat gtttggatgg ggacggtccg cccaatgtcg gtttccctgg cggccgacgt
     6001 ggcgttgcag gtacaggcaa cgcccgccac acagagtccg ccgcaaggca agcttctgac
     6061 cattgaagat gcagttcgca tcggcttgga aaaccaccct aggatccggg cagccaggga
     6121 aagaatcggc tcgcaggaag cgatactggg gcagcagatg tcggcctact atccaaccat
     6181 caatttcaac aattcctacc gcacgaccaa ttcatccgga accagcggga ccaattccag
     6241 cgcaacagat acttacacca gccaagccaa tctgaatctg acgttgtaca acttcggtaa
     6301 acgcgaaggc acagtccagg ccgcccggga aacggtggat gccacgcgcg aagacttctc
     6361 gacgacgacc caggatatcg ttctgagcgt caaacaggcc tactacactt atttaggcac
     6421 tcaagcgctg gtgacagtac gtcaggaaac ggtgcgtaac cgcgagcttc tggtacgtca
     6481 ggctcaagga ttttacgagg tcggtacgcg cgcgcgaatc gatgtcgctc gcgcggaagc
     6541 aaacctttat accgcgcgtg ccgatctcat cgcttctgaa aactcggtta aaattgcctg
     6601 ggttacattg agaaatgcca tgggatcgcc gcgcttaccc gagcagccgg tcgctcaaga
     6661 ttcgcctgag gtcgagctaa gcatgaactt ggcagccgca cgcactatcg cttttgacaa
     6721 ccggacggaa ttgcgtagct ttgaagcaca gcggcgggct tcggatcagt tgattgcgac
     6781 agcccgaaga ggccatctac ccgacatcgt tttcgacgca ttctatggcc ggagaaactc
     6841 cagtggaggt ggtgagagca cgtttcctct tcaacccgcc tggcaggcgc aggtgagtct
     6901 caatattccg atcttcgacg gttttcgcac aactaatcgg gtcgaggaga ctttacacaa
     6961 ttactacaat atcagagctc aggaagaaga gcggcggcag cagattgcct tggaagttga
     7021 gcagagctac ctcagggtgg tagaaactca ggagcggatt atagctactg aatccgcaac
     7081 cagggcagct aaagaaaacc ttgatttggc gcaagggcgt taccaggtcg gggtaggttc
     7141 gatcattgaa gtaaccgatg ccgagacttt atacaccgac gctcaaacga attatgtgcg
     7201 caatctttac gactacagaa ttgccgatgc tcaactggcg cgggcgatgg gcgatcaccg
     7261 cgttggagtt cttaagcctg ggtctataag gtagtttatg caaagtggcc ttggaggctc
     7321 gaggtaatga agcgtactct cttacttgtc atactgctcg tggtcatagg cgcttttgcc
     7381 ggcttccagc aatactcttc atggtcgttg aatcgttccc agaataatct cgaagttaaa
     7441 ccgcaagaag gcgcggtgca agcctcgcgc ggccaaagtc gccccggtaa tcagggccgc
     7501 ggcgga
//
LOCUS       DASUTG010000708         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1890150, whole genome shotgun sequence.
ACCESSION   DASUTG010000708 DASUTG010000000
VERSION     DASUTG010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890150"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..833)
                     /locus_tag="VF089_16400"
     CDS             complement(531..833)
                     /locus_tag="VF089_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175591.1"
                     /translation="MLRKRLALLVALSWVILSGYDVIEDLDLPDRIALHSSSDALSRG
                     AGAAGLLARNMVESADRPYLRCATLLEHHLEFAASYTPRVTQRVFKLHKVNQVFLI"
     gene            complement(962..1936)
                     /locus_tag="VF089_16405"
     CDS             complement(962..1936)
                     /locus_tag="VF089_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX6175592.1"
                     /translation="MSESNNGRWLLLIHQIPPKPNYLRVKIWRRLQRLGAVAIKNSVY
                     ALPKNDQAQEDFQWVLREIIEGHGDGTLCEARLIDGVTDDHVEALFQSARNADYAQIA
                     EDARLLGKTLRVKVKPAEPRRLQLEHEIVRLRKRFDDVIAIDFFGAPGREAASGLVSG
                     IEARIRGNKATAYSQTRMDREKLTDKIWVTRKGIHVDRMASAWLVRRFIDAEAKFKFV
                     PAKGYKPLSNEIRFDMFEAEFTHEGDRCTFEILIERVGIEDPALIPIAEIIHDIDLKD
                     GKFKRQEALGIERVIGGIAMSQKDDEIRLSRGAAVFNDLYEYFKRKRR"
     gene            2045..>3015
                     /gene="chrA"
                     /locus_tag="VF089_16410"
     CDS             2045..>3015
                     /gene="chrA"
                     /locus_tag="VF089_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6175593.1"
                     /translation="MQQTFERLSTSNPPTSETLRPGKGIGLWPLTGYFLKLGTIGFGG
                     PVALVGFMHRDLVEQRRWITEDTYKLSLALAQIMPGPLAAQTAIAIGYFEAGVTGATL
                     VGLSFVLPSFLMVIGISLLYVAYGGLWWMQALFYAIGATVIAIIAIAAYKLARSTNKR
                     DPLLWGIFALLALVTVWAQAELAEFFILAGLVVLFARAWPGWKRGLLMALAGVSLGLV
                     VWLIEAWVRQAGTGADPGDVLAQILLFFTKAGAFVFGSGLAIIPFLQQGVVQQFGWLN
                     EHQFLDAVAVAMITPGPVVITVAFIGFLVAGSGGAVMASIGIFLPVYL"
BASE COUNT          728 a          805 c          759 g          723 t
ORIGIN      
        1 gcttccgtcc agaatcggca cgagccagac atcttcctcg atcatggtaa gcgcggttct
       61 aaatactcgg tgatattcat cctttgaagt gggacccgtc tggttaccga ttcccggagc
      121 cgttataaaa actgcgaacg taaacacaac cgctaataag attgccctgt gagtatcaaa
      181 aaaatcagtc atgacgatga aagagtgaaa acggccccgt gcagcaagtc atcgagccgc
      241 gaaaaaagaa aaggcagagg agtcggcaaa cggcagaaga cgcgccaaac acctgctatt
      301 atccgcgaac gccagttaat ataacactga gtatagaacc tgagcagctg catacagcgc
      361 gcttgaacct acaatcaacc agacgacacc tttcaaccac aatggaaaat cagccatcac
      421 cactccttgc gtcgcgatgc gtttacgagc gaatcagccc gcagccgatg tgctctccgg
      481 gcgaacttaa aggaacttag aagggaaaga atgcaggaaa gacgaagcgc tcagatcaga
      541 aacacttggt ttactttgtg aagtttgaac actctctgag tgaccctcgg ggtgtagctg
      601 gcagcgaact caagatgatg ctccagcaag gtcgcgcaac gcagatacgg acggtccgca
      661 gattcgacca tgttgcgagc gaggagaccc gctgccccag cgccgcggga aagagcgtcg
      721 ctggaactat ggagcgcaat ccgatccgga agatccaaat cttcgatgac gtcgtatccg
      781 gaaagaatca cccatgaaag agcgaccaac agcgcgagcc gttttcggag catagagaat
      841 acgatactct accggtgcgc aaaagatgtc aacagtgata cttgcatgcg aaaatctaaa
      901 tccgccagtt acatttaagt ccctcaatca atgtaatgcg gttcactctc gaaatcgagt
      961 tctaacgacg tttcctcttg aaatattcgt acagatcgtt gaacacagca gcgcctcggc
     1021 tcaaacggat ctcatcatcc ttctgagaca tagctatgcc gccaatgacg cgctcaatgc
     1081 ctaaagcctc ctggcgttta aatttcccat cctttagatc aatgtcgtgg atgatctctg
     1141 caatcggaat tagcgcagga tcttcgattc ccacccgctc gatcaaaatc tcgaatgtgc
     1201 aacgatctcc ttcatgggtg aattcggctt cgaacatatc gaaacgtatt tcattgctca
     1261 gtggcttata ccctttagcg ggaacaaact tgaatttggc ctccgcatcg atgaaacggc
     1321 ggactaacca ggcgctcgcc atgcggtcga cgtggattcc tttacgggtc acccagattt
     1381 tgtctgttag cttttcacgg tccattctcg tctgagaata cgctgtggct ttgttgccac
     1441 gaatacgggc ttcgattccg gaaaccaaac cactagccgc ctctcgaccg ggagcaccga
     1501 agaaatcaat cgcgatcaca tcgtcgaagc gctttctgag tctgacaatc tcatgctcta
     1561 gctggagccg gcgcggttca gcaggtttga ctttgacccg cagagttttg ccaaggagac
     1621 gggcgtcttc ggcgatctgc gcgtaatccg cgttgcgagc agactgaaat agcgcctcga
     1681 catgatcatc agtcacacca tcaattaaac gcgcttcaca tagagtgcca tctccgtgtc
     1741 cttcaataat ttcgcgtagc acccactgaa agtcttcctg ggcctgatca tttttgggca
     1801 gagcatatac tgagttttta atggcgaccg cgccgagccg ctgtaaccta cgccagatct
     1861 ttacgcgcaa gtaatttggt ttaggtggaa tctgatgaat gagcaggagc caacgtccgt
     1921 tgttcgattc gctcatgaga aatttccttg actgaagcta cagctgttat atacgtgata
     1981 cagtaacagc accgggccat tcaaaatttt caaagcttct atcgctaaat agaacggatc
     2041 tattatgcaa caaactttcg agcgattgtc tacttcaaat ccaccgacaa gtgaaactct
     2101 ccgtcccgga aaagggattg gcctctggcc gctcaccgga tatttcctca aactaggcac
     2161 gataggattc ggcggcccgg tcgcattggt gggcttcatg catcgagatt tggtcgagca
     2221 acggcgttgg atcaccgagg atacttacaa actctcgtta gcgctggccc agatcatgcc
     2281 cggaccgtta gcagcacaaa cggctatagc tatcggatat tttgaagccg gggttacggg
     2341 cgcgaccctg gtcggactaa gcttcgtgct tccttctttt ttaatggtga tcggcatctc
     2401 gctcctctac gtcgcctatg ggggattgtg gtggatgcag gctctcttct atgccatcgg
     2461 ggccacggtg atcgccatca ttgctattgc cgcctacaaa ctcgcccgca gtaccaacaa
     2521 gcgcgatccg ctgctctggg gcattttcgc gctcctggct ctcgttactg tctgggcaca
     2581 ggccgaattg gcagagttct tcatcctcgc cggactggtc gttctcttcg cgcgcgcatg
     2641 gccaggttgg aaacgcggac tgctcatggc attggcgggc gtctcactag gcttggttgt
     2701 ctggttgatc gaagcctggg tgcgacaagc cgggacgggg gcggatcctg gagacgtgct
     2761 ggcgcaaatt cttcttttct tcactaaagc cggcgctttc gttttcggga gtggtctagc
     2821 gatcatacct tttttgcaac aaggcgttgt ccagcaattc ggctggctga acgaacatca
     2881 gttcctagat gcagtggcag tggcaatgat tacccctggt ccagtggtga ttactgtagc
     2941 tttcatcggc ttcctggtcg ccggctccgg cggcgccgtg atggcatcga tcggcatttt
     3001 tctgccggtc tatct
//
LOCUS       DASUTG010000709         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_703246, whole genome shotgun sequence.
ACCESSION   DASUTG010000709 DASUTG010000000
VERSION     DASUTG010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703246"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VF089_16415"
     CDS             complement(<1..929)
                     /locus_tag="VF089_16415"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6175594.1"
                     /translation="MSAHTVSTPTHERCVTSYAARTQYKPGEVRPPHIKVKDAIDIHC
                     HAHEGQQNPYDLARRASRAEMKGILFKSIPGDDPAKAVRDVQEDVDRWAEKEAVEPVR
                     CWSAYVCGRQMAPVTAGIVSEMIDRGVSAIWLPVFTHAITLSTVGTYQRFVDGNNAAG
                     WHGPIPWDKAVSIGHYNLDDKGELKPAVREIVRICADRNVALFFGHATHKEIYKLAEE
                     VEKLGLERAVIDHPYSPFLNVSPEMMKELSTIGIFFNFTWDELSPLLGVDPQIMYNTI
                     REVGPEHFTLSSDAGEPLFPDSVEAMRLVRGYM"
     gene            1090..2085
                     /locus_tag="VF089_16420"
     CDS             1090..2085
                     /locus_tag="VF089_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175595.1"
                     /translation="MKLRSITLVYAIAATLVHGSNALAADKVPFIYTARVMSQSYPWI
                     AQEAGLFKKYDLEVPLIFVTAGAPAVATILAGDSEVTQQGAAALSRAFVQGNRDVVFI
                     GGIKSILTHSIIAKPDIKTPADLKGKRIGVSRIGSNPHYFAVQALRHFGLESRDVSYI
                     QAGGAPETLAALLAQGIDAAVLTVPTDAQALKLGYHYVVYGPDLKIAYAATTFNTRRS
                     IMAKRGPVIGRFMRAMAEAAKIMHTDKEFTYKIMEKYLLVKDRKLLESSYNVEIKALE
                     PRLALKLEGFQSTLDEIAPTDPRAKTVKPQEMIDTRYLDEMEKSGFFDQIWGGKR"
     gene            complement(2348..>2642)
                     /locus_tag="VF089_16425"
     CDS             complement(2348..>2642)
                     /locus_tag="VF089_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX6175596.1"
                     /translation="LMQSWQEELSRLSARGQQNRMENIALSSSEITEAWTESGQDFIT
                     VRLNANLLDYTVDKRSGAVVSGSADEPIQFEEYWTFSRPVGPNAWKLTAVQQA"
BASE COUNT          571 a          703 c          748 g          620 t
ORIGIN      
        1 tccatgtagc cgcgcaccag gcgcatcgct tcgaccgaat caggaaagag aggttcgccg
       61 gcatcgctcg atagcgtgaa atgctccggg ccgacttcgc ggatggtgtt gtacatgatc
      121 tgcgggtcga cgccgagcag cggcgatagc tcgtcccatg tgaagttgaa gaagatcccg
      181 atcgtggaaa gctctttcat catctccggt gagacgttga gaaacgggct gtaagggtga
      241 tcgatgacgg cgcgctcgag cccgagtttt tcgacttcct cggcgagttt ataaatttcc
      301 ttatgcgttg catggccaaa gaaaagcgcc acgttgcgat cggcgcagat gcgaacgatc
      361 tctctgaccg cgggttttag ttcgccctta tcatctaaat tgtagtgacc gatactcact
      421 gctttgtccc acggaatcgg tccgtgccaa ccggctgcgt tgttgccgtc gacaaagcgc
      481 tgataggtcc caacggtgct caaggtgatc gcgtgggtga acacgggcag ccagatcgcg
      541 ctgacgcccc ggtctatcat ttcgctgaca atgcctgcgg ttaccggcgc catctgccgg
      601 ccgcatacgt aggcggacca gcatcgaacc ggctcgactg cttccttttc cgcccatcgg
      661 tcgacgtctt cttggacatc gcgaacagct tttgccggat catctcccgg aatgctcttg
      721 aataaaattc ccttcatctc cgcgcgtgat gcgcgccgtg ccaggtcata gggattctgc
      781 tggccttcgt gcgcgtggca atggatgtcg atggcatctt tgacttttat gtgtggcggc
      841 cgaacttcac cgggtttgta ttgcgtccgt gccgcgtaag atgtgacgca gcgttcatga
      901 gtgggagtag acacggtatg tgcgctcata tgtcctcctc ggaaggccat gcatgagttg
      961 tatcaatgac ccgctcgttt gcaaagcgaa attcatcgcc acatccatga tctgcttgac
     1021 cctggacctg catctagcta ttttcgcgca atcgaagggt ctgaagagaa gggcgcagga
     1081 ggagaaaaca tgaagttgcg atcgatcacg ttagtctatg caattgccgc taccttggtg
     1141 cacggctcaa atgcgcttgc cgcagacaag gtgccgttca tctatacggc tcgggtcatg
     1201 tcgcaatctt atccctggat tgcgcaagaa gcaggattgt tcaaaaagta cgatttggaa
     1261 gtgccgctga tctttgttac ggccggcgct ccggcggtgg caacgatact ggccggcgat
     1321 agcgaggtaa ctcagcaagg agcggcggct ttgagccgcg ccttcgttca gggtaatcga
     1381 gatgtcgtct tcatcggcgg catcaaaagc atactgaccc atagtattat cgccaagccc
     1441 gacatcaaaa cgccggcgga tctgaagggc aaaagaattg gtgtgtcgcg aatcggtagc
     1501 aacccgcatt attttgcggt tcaggcgttg cgccactttg gcttggagag ccgggacgtc
     1561 agttatattc aggcaggcgg cgcgcctgag acattggctg cgctgctcgc acagggtatc
     1621 gacgccgcag tgttaaccgt gcccaccgat gctcaggcgc tcaagcttgg ttatcattat
     1681 gtggtatacg gtcccgatct gaaaatcgcc tacgccgcca ccaccttcaa cacgcgccgg
     1741 tctatcatgg ccaaacgcgg tccggtgatc gggcgtttta tgcgcgctat ggctgaggcg
     1801 gccaagatca tgcataccga caaagaattc acttacaaaa tcatggaaaa atacttgctc
     1861 gtgaaagata gaaagttact cgaatccagc tacaacgtcg agattaaagc actagaaccg
     1921 cggcttgcgc tcaagctcga aggctttcaa tcgactctgg atgaaatcgc gcccactgat
     1981 ccacgggcga aaaccgtcaa gccgcaagag atgatcgata cgcgctatct ggatgagatg
     2041 gaaaagagcg gattctttga tcagatctgg ggcgggaaga ggtagggata aacggggatt
     2101 ccggagttag agattacaat gtgtactgaa ggccggtatt ttttcgtgga gtccgctgca
     2161 gtaccaggat cttgcaggcc gctacacgcc gctgaaccat tgcattcgct ctggagggct
     2221 tgccgcttga tcgttgtttc aatttgaacg ggctgtaagc cagtgctgct cttccgcaaa
     2281 aaaacagcga ccaggaccgt agtcctggtc gctgtgacag ctcttattgg aaagcttcgg
     2341 cgtgatgcta ggcttgctgt accgccgtta gcttccaagc attcgggccg acggggcggc
     2401 tgaatgtcca gtactcttcg aattgaatcg gctcatcagc gctgccggag accaccgcgc
     2461 cgcttctttt atcgacggtg taatcgagaa gattagcgtt tagccgaacc gtgatgaaat
     2521 cttgacccga ttcggtccac gcctcggtaa tctcactgga gctgagcgca atgttttcca
     2581 tgcggttttg ctggccgcgc gcgcttagcc ggctcaactc ttcctgccag ctctgcatga
     2641 gc
//
LOCUS       DASUTG010000710         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_110601, whole genome shotgun sequence.
ACCESSION   DASUTG010000710 DASUTG010000000
VERSION     DASUTG010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110601"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1028)
                     /locus_tag="VF089_16430"
     CDS             complement(51..1028)
                     /locus_tag="VF089_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6175597.1"
                     /translation="MTGRPPRLFLIDGSSYIYRAFFALPPAVNSAGFPINATYGFISF
                     MMKLMKRHQPDYLAVVLDAGRQTFRNQIFVEYKRNRRQIPNGLVLQLPHIRRVLDAFN
                     VTALELQNYEADDLIGTLCAILCDQHCDLIVVSSDKDLMQLITNKISLFDSAKERWIG
                     ANEVMARFGVEPERVPEVLGLMGDVVDNIPGIKGVGRKTAIALMQQFRNLEDLYGNLD
                     RLQQSNLRGVPRLREALQAGKETAFLSRDLATIKKNVPMQIDLEQLRFKGYSRDKMRS
                     LFAELGFNNLVKLVDYGREFTSDLGLFQPPQKTAPKESLPLQLQLFSWK"
     gene            complement(1071..>2451)
                     /gene="lpdA"
                     /locus_tag="VF089_16435"
     CDS             complement(1071..>2451)
                     /gene="lpdA"
                     /locus_tag="VF089_16435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6175598.1"
                     /translation="GAGPGGYTAAFYAADNGKKVILVEQSPRLGGICLNVGCIPSKAL
                     LHATEILREAKESANRGIGFGAAQINLDQLRSWKDSILEKLGQGIKGLAQKRGVQLIH
                     GRGHFEDSQTLRVETSEGQKFIRYEKAIIAVGSKPAMPSAFDLGNKRIMTSTEALDIQ
                     DIPHDLLVVGGGYIGMELGTVYAALGSHVVVLEALSSILTGVDPDLVRPVTRIAQKAF
                     KEIRVNTKVLKMATAGKQIKVTTQKDKEQREELYDRVLVSVGRAPNYSDLGLENTQVS
                     KDDKGFIRCNPQQQTDDPNIYAIGDVNGGALLAHRASKEAKVAIESILGHASAFDDVI
                     IPAVVYTNPEVAWCGLTESEAKDRGVAINVSKFPWGASGRALTLDRPDGLTKLLIEPE
                     TERILGVGIVGVGAGELISEGVLAVEMGATARDIADSVHPHPTLSETLMEAAEAFYGT
                     ATHTLSRRD"
BASE COUNT          566 a          665 c          603 g          617 t
ORIGIN      
        1 gctcctgaaa catttctcaa tatggagctc aatctgccac agtatgaact tcatttccaa
       61 gaaaataact gcagctgcaa gggtaggctt tctttcggag cagttttttg cggcggctga
      121 aagagcccga gatcgctggt aaattcacgg ccgtaatcca ccagcttcac cagattgttg
      181 aatccaagct ccgcaaataa ggatcgcatt ttgtctcggc tataaccttt gaatcgcagc
      241 tgttccagat cgatctgcat aggcacgttc ttcttgatgg ttgccagatc tcggctgaga
      301 aaagccgtct ccttacccgc ttgaagagct tcccggaggc gcggcacgcc acgcaggttg
      361 ctttgttgca accggtcgag attgccgtag agatcttcca gattgcgaaa ctgctgcatc
      421 aaagcgatcg cggtttttcg tccaacacct ttaataccgg gaatattgtc tacaacatca
      481 cccatcagtc ccaacacttc gggaaccctc tcgggctcta cgccgaatcg cgccatgacc
      541 tcgttcgcgc cgatccagcg ctccttggca ctatcaaata gtgaaatttt attggtgatc
      601 agctgcatca ggtccttatc gctggagact acgatcaagt cacagtgttg gtcgcacaat
      661 atcgcgcata gagtaccgat gagatcgtca gcctcataat tctgaagttc aagagcggtg
      721 acgttgaagg cgtccagcac tctgcggata tgcggaagtt gcagaaccag accattcggg
      781 atttgccgcc gatttctttt atactcgacg aatatctggt tgcgaaaagt ttgccggccg
      841 gcatcgagta cgactgcgag ataatcaggc tgatgacgct tcataagctt catcatgaaa
      901 cttatgaatc catacgtcgc gttgatggga aaacctgcgg agttaaccgc tggaggtaaa
      961 gcgaaaaagg ctcgataaat ataggagctg ccgtctatca gaaatagccg gggtggccga
     1021 ccggtcatgt gattgcgctt ctcgcgagat ccaaatttga gactactaaa tcagtctcgg
     1081 cgagaaaggg tgtgcgtcgc ggtgccataa aaagcttccg ccgcttccat caaggtctcc
     1141 gaaagggtgg gatgcggatg aacggaatcg gcgatatccc ttgccgtcgc acccatttcc
     1201 actgccaaaa ctccctcgct gatcaactca ccggcgccaa ctccgacaat gccgactccg
     1261 agaattcttt ccgtctccgg ctcgatcagc aacttggtga ggccatccgg gcgatccaaa
     1321 gtgagcgctc tacccgacgc gccccaggga aacttcgaga cgtttattgc aacgccccta
     1381 tctttggctt cgctctctgt taacccgcac caggcaactt caggattagt ataaacgact
     1441 gctggaatga tgacgtcatc aaacgcgctc gcatggccca ggattgactc aatggcaacc
     1501 ttggcttctt tggatgcgcg gtgcgctagt aaggcgccgc cgttgacatc accgatagca
     1561 tagatgttgg ggtcatcggt ttgttgttgc gggttacacc gaataaaccc tttgtcgtct
     1621 ttactgacct gtgtattctc aagaccaaga tcactgtagt tgggtgcccg gccgacgcta
     1681 accagaacgc gatcataaag ctcttcgcgc tgttccttgt ctttctgcgt cgtcactttg
     1741 atttgttttc ctgcggtggc catcttcaaa actttagtgt tgacgcggat ttctttgaac
     1801 gccttttgcg cgattctagt caccggtcga accagatcag gatcgacgcc ggtcagaata
     1861 gaggataggg cctcaagcac gacaacatgg ctgccaagcg ctgcataaac ggtccctagt
     1921 tccatgccaa tgtagccgcc cccgaccacc agcagatcgt gtggaatgtc ttggatgtcc
     1981 aacgcctcgg tggatgtcat gatgcgctta ttgcccaaat cgaaggcgga gggcatcgcc
     2041 ggcttcgaac cgacggcgat tatagctttc tcatagcgga taaacttctg accttcggat
     2101 gtttcaactc tcagtgtttg tgaatcttcg aaatgacccc gaccatggat taattgaacg
     2161 ccacgttttt gcgccagtcc ctttattccc tgccccaact tttccaaaat cgaatccttc
     2221 caactgcgca gttgatcgag attaatttgc gcagccccga acccgatacc acgattggct
     2281 gattctttgg cttcccgcag gatttcggtg gcatgtaaaa gtgcctttga gggaatgcat
     2341 ccgacgttta gacaaatgcc gccgagccgt gggctttgtt cgactaagat cactttttta
     2401 ccattgtctg ccgcatagaa ggctgccgta taaccgcctg gcccggcgcc g
//
LOCUS       DASUTG010000711         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1591040, whole genome shotgun sequence.
ACCESSION   DASUTG010000711 DASUTG010000000
VERSION     DASUTG010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591040"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..505
                     /locus_tag="VF089_16440"
     CDS             122..505
                     /locus_tag="VF089_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6175599.1"
                     /translation="MYITIDETDQRPIYQQIVDEIKAIIAAGELPEGSSLPPVRQVAA
                     DLGVNQNTVAFAYRELQKSGLIRVRHGSGAIVIRRLTEKRDQDQLEAKLTPVLTDMVL
                     AGLTRAEVNALVNNVLNGLVGTPSR"
     gene            complement(604..1683)
                     /locus_tag="VF089_16445"
     CDS             complement(604..1683)
                     /locus_tag="VF089_16445"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX6175600.1"
                     /translation="MRKASFVLFPILAAIAVVLLPIDGLAQNPRDRFVISARAGGVNA
                     VTGGATVRTHGKTEWEQLTIKEDLQTGDSVKTGNDGRVEMLLNPGSFLRVGENSEFEL
                     ANSSLDNLEIRLVRGTAIVEAGGADDSRMQINISTPHTKIAIVRRGLYRLNVVPGDNT
                     ELIVRKGRVMLDKTKIGGGNKVVFSADNFLVAKLQNADKKKVDGFDSWSKERSEVVAE
                     ANRSIRRKEVSALMASARDRWFNIAFSAANPGFWYFSPRFSCYTFVPYYGGWGSPYGY
                     SYSSAFFPRYYCGGCRGLIDYRNSPGGTTAASSGSFGGGSSGGSSSAPGTVTPVPSSR
                     PSMEGLSRPSAMEGRILRHQDRPNN"
     gene            complement(1798..>2580)
                     /locus_tag="VF089_16450"
     CDS             complement(1798..>2580)
                     /locus_tag="VF089_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6175601.1"
                     /translation="DFRLYENGSEQNILQFESAAAPFDLLLLIDISGSTREKLELIRA
                     AALRFVAAARSQDRIGVITFAGSPALVSRPTLDRRLLRDRINAIETARLGDTKLYDAT
                     DFALSQVLQEAKSRRRTAVILMSDGLDGAIEGVRGDGSATPYRELVNRIQEFDGVLYT
                     LWLNTRYVPLSAEDTSEEAFDQGYDQMKEIADVGGGVFYEVKRLEDLAGAYERVVADL
                     GTVYSLAYRPSEKNRDGRWRAIRVSVQRPSAVARGKRGYYAN"
BASE COUNT          607 a          737 c          671 g          565 t
ORIGIN      
        1 gacacatgct cccacggagg catagcaggc gtggcaggca acgccctttt gctggccaat
       61 cacctttcaa aatagtgctg gacaaagacg aattgtcgag tgtatacatg caccagacaa
      121 tatgtatatt actatcgacg aaaccgacca acgacccatc taccaacaaa tagttgacga
      181 aataaaggcg attattgctg cgggggagct tcccgaaggt tcttccctgc cacccgttcg
      241 acaagtcgcg gcggatctcg gcgttaacca gaacactgtg gcttttgcct atcgagaact
      301 gcagaagtca ggcttgatta gggtgaggca tggctcgggc gctattgtga ttcggcgtct
      361 gacagagaaa cgagaccagg accaactcga ggctaaactc acgccggtct tgacggacat
      421 ggttctggca ggcctcactc gcgcggaagt caatgccctg gtcaacaatg ttctgaatgg
      481 attggtcggt acgccgagtc gctgacccag tgtgagcaag cttttgaggc agaatgtacc
      541 gagcataaaa aagcggcgcc gagttgttgc tcgacgccgc taactgatgc tcggttcgct
      601 agtttaatta ttcggccggt cctggtggcg caggatcctg ccttccattg cgctgggtcg
      661 cgataagcct tccatcgaag ggcgcgaact ggggaccgga gtaacggtac caggtgccga
      721 gctcgagcct cccgaacttc caccgccgaa actacccgaa gaagctgccg tagtaccgcc
      781 ggggctattc cggtagtcaa taaggccgcg gcaaccaccg cagtaataac gtgggaagaa
      841 cgcactcgaa tagctgtaac cgtagggcga accccaaccg ccgtagtagg gtacgaacgt
      901 gtaacaactg aatctcgggc tgaagtacca aaaaccagga ttcgcagctg agaatgcaat
      961 gttgaaccaa cggtcgcggg cactcgccat aagcgccgat acttccttgc gccggatact
     1021 gcgatttgcc tcagcgacta cttctgagcg ctctttgctc caggagtcga agccatcgac
     1081 cttcttctta tcggcgtttt gaagcttggc caccaggaag ttatccgcgc taaacaccac
     1141 tttattgccg ccgccgattt tggttttatc caacatcacg cggcctttac gcacaatcaa
     1201 ttcggtgttg tcaccgggta ccacgttcag ccggtaaagc ccacgccgga cgatcgcgat
     1261 tttcgtgtgc ggagttgaaa tgttgatttg catccggcta tcgtcagcac cgcccgcctc
     1321 aacgattgcc gtcccccgga ccagccgaat ctcaaggttg tccaacgagc tattcgctaa
     1381 ctcaaactcg gaattctcgc ccactcttaa gaaagaacca ggattcaaaa gcatctcgac
     1441 ccggccgtcg ttgccggttt tgacgctgtc accggtctgg agatcctctt tgatggttag
     1501 ctgctcccat tccgtcttgc cgtgtgtgcg taccgtcgca ccgccggtga ccgcattcac
     1561 gccgccggcg cgggcagaga tgacaaatcg atcgcgcggg ttctgagcca aaccgtctat
     1621 gggaaggaga actacagcga tggctgcaag tattggaaag aggacgaaac ttgcttttct
     1681 catgagaatt tcctttgttt tgggaccggt aaaatcagaa ctgtctgggg aggccaatta
     1741 tattcctatc cccgacattt acaagccttt ggataagccg ggtaaaattt aacaaaacta
     1801 atttgcgtag tacccacgtt tgccccgtgc caccgcggat ggacgctgaa cgctcacccg
     1861 gattgcacgc caccttccgt cgcggttttt ctcactgggc ctgtacgcca gactataaac
     1921 agtgccgaga tccgcgacga cccgctcata cgcgcccgcg agatcttcga ggcgctttac
     1981 ttcgtagaac accccgccac caacgtctgc gatctctttc atttgatcgt agccttgatc
     2041 gaaagcttct tcagaggtgt cttcggcgct aaggggtacg tagcgcgtgt tcagccataa
     2101 cgtataaaga accccatcaa actcctgtat tcggtttacc agctcgcgat atggcgttgc
     2161 cgaaccatcg ccccgcactc cttcgattgc accatccaaa ccatcactca tcagaatgac
     2221 ggcggtgcgc cgccggcttt tagcttcctg cagaacctga gacaacgcga aatctgtggc
     2281 atcgtagagc ttagtatcgc caaggcgagc cgtctcgatc gcgttgatcc gatcacgcag
     2341 tagcctgcga tccaaagtcg gacgtgaaac aagcgcggga cttccggcga aagtgatcac
     2401 accgatgcgg tcctgcgacc tcgctgctgc gacaaagcgc agcgcggcgg cacgaatcaa
     2461 ctccaacttt tctcgagttg aaccagagat gtcgatcaag agcaacagat caaacggggc
     2521 tgcggccgac tcgaattgaa gaatgttctg ctcggaacca ttctcgtaaa ggcggaaatc
//
LOCUS       DASUTG010000712         7945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_850750, whole genome shotgun sequence.
ACCESSION   DASUTG010000712 DASUTG010000000
VERSION     DASUTG010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850750"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="VF089_16455"
     CDS             <1..1894
                     /locus_tag="VF089_16455"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX6175602.1"
                     /translation="ALVNLEVTDNDTVSGPKLGSSRSIRLRLKNIKGEHQQIAEMVRE
                     LNSRMVDLLGDHLENPLPEENNAARTPDVNQKFEQNLAEALKQTEEVMRRTEKDRLSD
                     FATWSDLEALRRNLQFTKEELLKKQEQAQSADDKLRARDEISSELERMSLLSEDISKR
                     LRAQEMASTAQDLARSQERLMDSLEKLQSGDKNLDSLLKQLSELTKSLAALQQAMSQM
                     AQQLPDDFMNMESMQSLGFNEMFSALDEIRKKLMQGDIEGARQLARELFNQMAQMVAS
                     LQNSQRSAMASSMGRMQGEMSRSTNELQQIAREQQEILLNTEDINKSALSERDSALKE
                     KLDRFLENAKSELGKLTELFPDREGPEDPEAATKLDDATMNQLLKNLIAKLLSKDFPG
                     YGEGEGLARKELAKKRDPSQMDRARRAESTLNQLKSELDSLIGQPMQALKESDQKQLR
                     DLANRQGVLRERTESLHEKLESLFQLFPSLDPKIIQNIGEAGKSMNNANDRLGRLDSQ
                     GAVPPERDALDRLSESQQQMQSAMQQMAQRGQLGNMPVTRLFRQGRFLPGGMLTPLPG
                     MPQFPEFEVESGFTGLDTEKFRLPGKEEYKAPRNFREEILESLKQGVPPQLKDQIERY
                     FKNLSE"
     gene            1919..3487
                     /locus_tag="VF089_16460"
     CDS             1919..3487
                     /locus_tag="VF089_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6175603.1"
                     /translation="MAPIVIWLGLTVQLFAAQVEDKFKEGQELLDAWRISQAEELAVK
                     SLRDNPKSADALDLDARVKFYQGRYQEALASIDRALAIDSKEKQRQGLKLLSQFTVDV
                     IKTLKRYESAHFVLYLDEKRDGILATHALDALEKSYQAVGAELGYYPKEKVRVEIAPD
                     APSFNAISTLSLRDIEETGAVGICKFNKLMIISPRALSLGYRWVDSLSHEYLHYAIVG
                     LTNNQAPIWLHEGMARFYETRWRKPMPPKDAAEDYLTPANQTLLVQALEKNQFVGFKK
                     MEPSLIHLETPEQVQLAYAEAASAIDYINQSKGKAGVREFLATLNEAPTAEAISKVFG
                     LSFDAFEAQWKNFLKQKGLKEIEGTRVRRLKVKKDQNEDQEIVELKEIQSAVARNRTH
                     LGDQLSNRGRTVAAANEYQRALQASPHSPIILNKLGRVLIEMNRYSEALTPLKRALEL
                     DPDNGGTYVQLGRIHHATKNFHESKNALEEAIQINPFNPLIFKLLTEVYASSGEQEKS
                     QQAKASLDKLLGAN"
     gene            3500..4528
                     /locus_tag="VF089_16465"
     CDS             3500..4528
                     /locus_tag="VF089_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6175604.1"
                     /translation="MDPAKIHQHEIAEIEEFAAKRDTMLGEIRKVIIGQDRVVEEVLI
                     ALFARGHCLLVGVPGLAKTLLISTLAEILDLEFNRIQFTPDLMPSDITGTDILQEDPS
                     SGRRQFQFLKGPIFTNILLADEINRTPPKTQAALLQSMQEYKVTAGGTTFPLDLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMLNIEIGYPDFEDEVQIVLQTTSNEKPAPRKVMEG
                     AAILRYQELVRRVPVSPFVVSYAVALSQSSRPQNGDAPQFVRDYVEWGAGPRASQYLI
                     LGAKARTILQGRYAVSIEDIQALASSVLRHRIVPNYKAQGEGLSSVDIIKRLISEVKP
                     PKDGKTAL"
     gene            complement(4701..5822)
                     /locus_tag="VF089_16470"
     CDS             complement(4701..5822)
                     /locus_tag="VF089_16470"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6175605.1"
                     /translation="MLVVPSVALTPPAQAQAPRSPTPAPVQGALKVENVARGLEHPWS
                     LAFLPDGRMLVTERPGRLRLVSSGGQLSEPLSGVPQVYARGQGGLLDVVLSPAFANDR
                     LIYFSYAEAGEGGAGTAVARSRLGERGLENTQVIWRQVPKVSGSNHWGSRIVFRSDGT
                     LFVTLGERFSYSDSAQDLSTTLGKIVRINADGSAPPDNPFVKRAGARPEIWSYGHRNV
                     QAAALHPENGQLWTVEHGARGGDELNHPEAGKNYGWPVISYGVHYSFFRIGEGTAKEG
                     MEQPVYYWDPVIAPSGMVFYTGDLFAGWKNDILIGSLTPGLLVRLALKGGQVAREERY
                     LGNLRERIRDVRQAPDGSVYLVTDARNGQILRITPAGSG"
     gene            complement(5997..6254)
                     /locus_tag="VF089_16475"
     CDS             complement(5997..6254)
                     /locus_tag="VF089_16475"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX6175606.1"
                     /translation="MQLSLQRRTSYSRDDGDERSPVKPDEAFYSDDLREFILPYDRLR
                     ESESPDETLLQFLQTTYEAAANLAQWDRAGAGAQRAPEERT"
     gene            complement(6337..6810)
                     /gene="rplQ"
                     /locus_tag="VF089_16480"
     CDS             complement(6337..6810)
                     /gene="rplQ"
                     /locus_tag="VF089_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX6175607.1"
                     /translation="MRHLNSGRKLSRNSSHRWAMMRNMVTSLLREEKIQTTDPKAKEL
                     RRWVDRVITLGKEGSLHARRQALAIIQDKTVVHKLFDTIASRFQNRPGGYTRIIKIGR
                     RHGDAAPLSLIELVAGADEAADATSGKDKKAKKRSGKTAAKAGSGRRKNAKAASA"
     gene            complement(6812..7831)
                     /locus_tag="VF089_16485"
     CDS             complement(6812..7831)
                     /locus_tag="VF089_16485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX6175608.1"
                     /translation="MYRHWTDLIKPKQLEVDEKTLTSTYGKFYAEPFERGFGQTIGNS
                     LRRILLSSLMGASIVAVRMKGILHEFSTIPGVTEDVTDIILNLKEVRLRLNDGEQHTL
                     KVEAKGPATVLAKDITGSANVEILNPEQKIATLSRDAKLDMELVAKLGRGYVPAERNR
                     EEGAPVDTVFIDAIFSPVQKVNFTVTNARLGQRTDYDRVVFEVVTDGSVKPDDAMAYA
                     AKILQDQVSIFVNFQDEPQQERRDDRPSIPLNDNLFRSVDELEFSVRSQNCLQNADIK
                     YIGELVQKSEQEMLKTKNFGHKSLNEIKEILREMGLELGMKIDHFPSREEIESRRRAD
                     EKETA"
BASE COUNT         1904 a         2202 c         2114 g         1725 t
ORIGIN      
        1 cgcgctggtc aatctcgaag tcaccgataa tgacacggtt tccggaccca agctgggctc
       61 atcgcgctcg atcaggctgc ggctgaagaa tatcaagggc gagcatcagc agattgccga
      121 gatggtccgt gaacttaata gtcgaatggt cgatctgctg ggtgatcatc tggaaaatcc
      181 gctgccggaa gaaaacaacg cggctcgcac ccccgacgtg aaccagaaat ttgagcagaa
      241 tcttgccgag gcattgaagc agaccgaaga agtgatgcgc agaaccgaaa aggaccggct
      301 ctccgacttc gcgacctggt ccgatctcga agcgttgaga cgaaatctgc agttcaccaa
      361 ggaagagttg ctcaaaaaac aagagcaggc ccagtctgct gatgataaat tgagagcccg
      421 cgatgagatc tcgtcggaac tggagcgcat gtcgctgctt tcggaagaca tcagtaaacg
      481 gctcagggcg caggagatgg cctcgacggc gcaggacctg gcgcgcagtc aggagcgcct
      541 catggattct ttagaaaagc ttcagagcgg cgacaagaat ctggattcgc tacttaagca
      601 gctttccgag ctcaccaagt cactggccgc gctgcagcaa gccatgtcgc agatggcgca
      661 acaactgccg gacgatttca tgaacatgga aagtatgcag agcttggggt ttaacgagat
      721 gttttcggcc ctcgacgaga ttcgcaaaaa gctcatgcaa ggcgacatcg aaggggcgcg
      781 gcagctagct cgggagttat tcaatcagat ggcgcaaatg gtcgcttcct tgcagaactc
      841 gcagcgttcg gcgatggctt caagcatggg tcgtatgcaa ggggagatgt cgcgttcaac
      901 caacgaactg cagcagatcg cgcgcgagca gcaggaaatc ctcttgaaca cggaagacat
      961 caataagtcg gcgttaagcg agcgcgatag tgctttgaag gaaaaactgg atcgattcct
     1021 ggaaaacgcc aagagcgagc tgggaaaatt gaccgagctc ttccccgatc gcgaggggcc
     1081 ggaagatccc gaagccgcca ccaagctcga tgacgcgacg atgaatcaac ttcttaaaaa
     1141 tttgatcgca aagctgctga gcaaggattt tccgggctat ggcgaaggcg aagggttggc
     1201 ccgcaaagag ctagcaaaaa aacgcgatcc ttcacagatg gacagggcac ggcgtgctga
     1261 aagcacgttg aaccagctca agtccgagct ggacagtttg atcggccaac cgatgcaggc
     1321 gctcaaggaa tccgatcaaa aacaactgcg cgatctcgcc aaccgtcaag gggttttgcg
     1381 cgaacgcact gaatccctgc acgagaagct ggagtcgctt tttcaacttt tcccatccct
     1441 ggatccaaaa atcatccaga atatcggcga agccgggaag tccatgaaca acgccaacga
     1501 ccggctcggc cggctcgatt cgcaaggcgc cgtaccgcct gaacgtgatg cgctagaccg
     1561 gctttcggag tcgcagcaac aaatgcaatc ggcaatgcaa caaatggctc agcgcggcca
     1621 acttggcaac atgccggtca cccgcttatt ccgtcaaggg cgcttcctgc caggtggaat
     1681 gctgacgccg ctgccgggca tgccgcagtt tccggagttt gaggtcgaga gcggtttcac
     1741 cggactggat accgagaaat ttcgtcttcc cggcaaagaa gaatataaag ctccgcgaaa
     1801 cttccgcgaa gaaattctgg agtctctcaa gcaaggcgtt ccgcctcagc taaaggacca
     1861 gatcgaacgc tatttcaaaa atctttccga gtaatagcca ttgaaatatc gaacgttctt
     1921 ggcaccgatt gttatttggc tcggcttaac ggtgcaattg tttgccgccc aagtcgagga
     1981 caagttcaag gaggggcaag agctcctcga cgcgtggcgc attagccaag ctgaagagct
     2041 cgccgtaaaa tcattgcgtg acaacccgaa gtccgccgat gcgctggatc tcgatgcccg
     2101 ggtaaagttc tatcaaggcc gttatcagga ggctttggcc tccatagacc gggctctggc
     2161 cattgattcg aaggaaaagc agcgccaagg attgaagctg ctgagccagt tcactgtgga
     2221 cgtgatcaaa acgcttaaac gttacgagag cgcccatttc gtactttacc tcgatgaaaa
     2281 acgcgatggg atactggcaa cccatgccct cgatgctttg gaaaagagct accaggcggt
     2341 aggcgcggag ctcggttatt accccaaaga aaaagtccgc gtcgagatcg ccccggatgc
     2401 tccgagcttc aacgccatct caactctctc gctgcgcgat attgaagaaa ccggcgcggt
     2461 gggtatctgt aagttcaata aactcatgat catctcaccg cgcgcgctga gtctaggtta
     2521 tcgttgggtc gattccctaa gccatgagta tcttcattat gccatcgtcg gtcttaccaa
     2581 caaccaggcg ccgatctggc tgcacgaggg aatggcgcga ttctacgaaa cccgctggcg
     2641 caaaccgatg ccgcccaaag acgcagccga ggattatcta acgccggcga atcaaacgtt
     2701 gctggtgcaa gctctggaga aaaaccaatt tgtcggattc aagaagatgg aaccttcgct
     2761 gatccacttg gaaacgccgg agcaagttca gctggcctat gccgaggctg cgtcggcgat
     2821 cgactacatc aatcaaagca aaggtaaagc cggcgtacgg gagttcttgg cgacccttaa
     2881 tgaagcaccc acagccgagg cgatttcaaa ggtgttcggc ctgtcgttcg atgccttcga
     2941 ggcgcagtgg aaaaatttcc tcaaacaaaa agggctcaag gaaatcgaag gcacccgcgt
     3001 gcgcaggctc aaagtcaaaa aagatcaaaa cgaagaccaa gaaatcgtcg agctcaagga
     3061 gatccaatcg gcggtcgcgc gtaaccgcac ccaccttggc gatcaactat cgaatagggg
     3121 ccgaacagta gcggcggcta acgaatacca gcgcgccttg caggctagcc cgcactctcc
     3181 gatcattctc aacaagctgg gacgggtttt aatcgaaatg aatcgctact ccgaggcgct
     3241 gactccgttg aagagagcgc ttgaactgga tccggacaac ggcggcacct acgtacaact
     3301 cggccgcatt caccacgcga cgaaaaattt tcacgagagt aagaacgcgc ttgaggaggc
     3361 aattcagatc aaccctttca atccgcttat ttttaagcta ctgaccgaag tctacgcatc
     3421 ctcgggcgag caggaaaaat cgcagcaggc gaaggcttcg ctcgataagt tgctcggcgc
     3481 caactgaaag ggaacctgga tggacccggc taaaattcat caacatgaga ttgcggagat
     3541 cgaagaattc gcggcgaaac gcgacaccat gctcggagaa atccgcaagg tcatcattgg
     3601 tcaggatagg gtcgtcgaag aagttttgat cgcgctgttc gcccgcgggc actgcctgtt
     3661 ggtgggcgtc ccgggcttgg ctaagacgct gttgatcagc accttggcgg agatcctcga
     3721 tcttgaattc aatcggattc agtttacccc cgacctcatg ccctccgaca tcaccggcac
     3781 ggacattctt caggaggacc catcgagcgg caggcggcag tttcaattcc ttaaagggcc
     3841 gatattcacc aacattctgc tggctgatga gatcaaccgc acgccgccca agacccaagc
     3901 cgcgctgctg cagtcgatgc aggagtacaa agtcaccgct ggcggcacaa cttttccgct
     3961 ggatctgccg ttctttgtcc tagcaacgca gaatcccatc gagcaggaag gcacctatcc
     4021 ccttcccgaa gcgcagctcg accggtttat gcttaatatc gaaatcggtt atccggattt
     4081 cgaggacgaa gtgcaaatcg ttctccagac gacttccaat gaaaaaccgg cgccgcgcaa
     4141 ggttatggaa ggcgctgcga tcctgcgcta tcaggaactc gtcagacgtg ttccggtctc
     4201 gccgtttgtg gtttcttacg cagtggcgct gtcgcaaagt tcacggccgc aaaatggcga
     4261 tgcaccgcag ttcgtcagag attatgtcga gtggggcgcc gggccgagag cttcgcagta
     4321 tctgatcctc ggcgctaagg cgcgcacgat tctccaaggc cgctatgccg tttccatcga
     4381 agacattcaa gcgcttgcgt cttcggttct acgccaccgg atcgtgccga actataaggc
     4441 ccaaggcgaa ggattgtcat ccgtagatat tatcaagagg ctgattagcg aagtgaaacc
     4501 gcccaaggac gggaaaacgg cgctgtgaag acgtcgtctc aagactcttc gttgcgcggt
     4561 cggctgagcc ccaagcaggt gtggtacttc gcggaagcaa tgttgtacga gttagaaatt
     4621 aattcgatga tatcgcgtca cccaacaatt aaccttcgtt atctctcatt gtttaatgca
     4681 ccggtttgaa cgatctgctt ctaaccgctg cctgccggcg tgatccgaag gatctgaccg
     4741 ttgcgcgcgt ccgtaaccag atagaccgaa ccatccggcg cctggcgaac atcccggatg
     4801 cgctctcgca agttcccaag gtagcgttct tctcgcgcaa cctgaccgcc ctttagcgcc
     4861 aggcgcacta ggaggcccgg agtaagcgag ccgattagaa tatcattttt ccaacccgcg
     4921 aacaggtcgc ccgtgtagaa gaccataccc gaaggcgcaa tcaccggatc ccaataatac
     4981 accggctgct ccatcccttc tttcgctgtg ccttcaccga ttctaaaaaa cgagtagtgc
     5041 acaccgtagg aaatcaccgg ccagccataa tttttgccgg cttccggatg atttaactcg
     5101 tctccgccgc gcgcgccgtg ctcgacggtc caaagctgtc cgttctccgg atgaagcgca
     5161 gccgcttgaa cgttgcgatg cccatacgac cagatctccg gtcgcgctcc ggcgcgtttg
     5221 acgaatgggt tgtcaggggg agctgaacca tcggcattga tccgcactat cttaccgagg
     5281 gtcgttgaca ggtcttgggc gctatcggag taactaaacc gctcgcctaa agtcacgaac
     5341 agggtgccgt ctgaacgaaa cacgattctc gatccccagt ggttggagcc gctgaccttg
     5401 gggacctggc gccaaatcac ctgggtgttt tcaaggccgc gctcacctag ccggctcctc
     5461 gcaacagcgg ttcctgcgcc accttcccca gcttcggcat atgaaaaata gatgagacga
     5521 tcgttagcga atgccgggct cagcacgaca tcgaggagtc cgccttgccc gcgcgcgtaa
     5581 acctgaggaa ctccggacag cggttcagac aactggccgc cactgctcac gagacgcaag
     5641 cgacccgggc gctcggttac aagcatccgg ccgtccggta ggaaagcgag actccaggga
     5701 tgttcgaggc ctctggcaac attctcaacc ttgagagcgc cttggacagg cgccggtgtc
     5761 ggcgagcgcg gcgcctgagc ttgtgccggc ggcgtaagcg cgactgaggg cactacgagc
     5821 acgaggtact tgagcctcgg acaaatcttg tttaacagcc gccgaaccat acaagtgagt
     5881 tggagtcaat caaactccac aatcctgcgc gtcttcaatg actctacaag gcgcggccct
     5941 caatctgagt cgttatcaac ctatcaatca atggatgcgt agcgtctgat tgagctctac
     6001 gtccgctctt cgggtgctcg ctgcgctcca gcgccggcgc gatcccattg tgccaaattg
     6061 gccgctgctt cataggtggt ctgcagaaac tggagcaagg tctcgtcagg cgattccgat
     6121 tcgcgcaacc ggtcgtacgg taaaatgaat tccctcagat cgtcactgta gaaagcctcg
     6181 tccggcttca caggcgagcg ttcatcaccg tcatctcgac tgtacgaggt tctgcgctga
     6241 agtgaaagct gcagatgaag agtgaaccaa aaaaagccgg agtcatcgag atctaactcg
     6301 accggactcc ggctgagatc tcataccggg agagttctag gcggaagctg ccttggcgtt
     6361 cttccggcgg ccggaacctg cttttgcagc cgttttgccg cttctcttct tcgctttctt
     6421 gtctttcccg ctcgtggcat cggctgcttc atcggcgccc gcaaccaact ctatcaatga
     6481 tagcggcgct gcatctccat gccgccgacc gattttgatg attcgagtat aaccgccagg
     6541 acgattctgg aagcggctgg ctattgtgtc gaatagttta tggaccacgg tcttatcctg
     6601 aataatggcg agcgcttggc gtctggcatg gagacttccc tccttgccga gggttataac
     6661 ccggtccacc cagcgtctta gctctttggc cttgggatcg gtggtttgta tcttctcttc
     6721 acgcaaaaga gaagtcacca tattgcgcat catcgcccag cggtggctcg aattgcggct
     6781 cagctttctt cccgaattaa ggtgtcgcat cttaggcggt ctctttctcg tcggcgcgcc
     6841 gtctgctctc gatctcctcc cgcgatggaa aatggtcgat cttcatgccg agctcaagac
     6901 ccatttcgcg caggatttct ttgatctcat tgagggactt gtgaccgaaa tttttggttt
     6961 tgagcatctc ctgctccgac ttctgcacca actcgccgat gtatttgata tcagcgttct
     7021 gtaaacaatt ttgcgagcgc acggaaaatt ctagctcgtc tacgctgcga aagaggttat
     7081 cgttaagcgg gatagacggc cggtcatcgc gtcgttcttg ctgcggttca tcttgaaagt
     7141 taacgaagat actcacctga tcctggagga tctttgcggc gtatgccatc gcatcatccg
     7201 gcttaacgct gccgtcggtt accacttcaa acacgactcg atcataatcg gtgcgttgcc
     7261 cgaggcgagc attagtaaca gtaaaattga ctttctggac cggcgaaaag atggcgtcga
     7321 taaataccgt atcaacgggc gccccctctt ccctgttcct ttccgccgga acgtagccgc
     7381 gccccagctt cgctaccagt tccatatcga gcttagcatc tctggagagc gtggcaattt
     7441 tttgttctgg gttgagaatc tctacattgg cagatcccgt gatgtcctta gccaacacag
     7501 tggccggacc cttggcttca acttttaatg tatgttgctc gccatcatta agtcgcaacc
     7561 ttacttcctt gagattcaaa atgatgtccg taacatcttc cgtaaccccg ggaatcgtcg
     7621 agaactcatg aagaattcct ttcatccgga cggcgacaat cgaagcaccc atcaaagacg
     7681 ataacaaaat tcgtcgtagc gaattaccaa tggtttgtcc gaagccgcgc tcaaagggct
     7741 ccgcataaaa ctttccgtac gttgaagtta aggtcttttc gtccacctcc aactgctttg
     7801 gtttaatcaa atctgtccaa tgcctataca tagtagacat agtttctccc tacattcaga
     7861 agcttacttc gaatacaatt cgacgatcag cttctcgtta atcggcatgg taatgtcgct
     7921 gcgggtgggt agaatcttga ttctt
//
LOCUS       DASUTG010000713         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_703762, whole genome shotgun sequence.
ACCESSION   DASUTG010000713 DASUTG010000000
VERSION     DASUTG010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703762"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VF089_16490"
     CDS             <1..1019
                     /locus_tag="VF089_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175609.1"
                     /translation="EYAVIPLFLIWGGLLWFDVDVRFRPDVHTPSQQGLAQKLREFSR
                     ELQEKAGSEGLRRSLQVGRELEKAAQKSIEAKIADEQFKSEISGMSSKVEAMGKSMGN
                     EPSFATSESTQNLKDLKAELEAAQELLKLPDGAKGERGLGHSSLDRLKTMPQLNRQLD
                     QQGQTNRSPSQNELRSFLDSMDKQVTGELDRRTLLDAQQFLDQLAKQGHGDKAETGAR
                     VAGRGEEDLPGEGEKAQSNSNRPGSEPGTKEEIQPAPQLPPAAATQLKGMIGQGNSSG
                     VGLKGKPSPGKTEVSQEEVIATYQRQAEQELNTERVPEALKETIRRYFLSLGNKQRSD
                     EPGK"
     gene            1093..2109
                     /locus_tag="VF089_16495"
     CDS             1093..2109
                     /locus_tag="VF089_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX6175610.1"
                     /translation="MESVAEIERRIQSARRSFDEVKAEMARVIVGHEDLIEDIFIALV
                     CGGHCLLEGVPGLGKTLLVRSLGEILSLSFSRIQFTPDLMPADITGTNMLSDDPSGRK
                     VFEFQRGPVFAHIVLADEINRATPKTQSALLEAMQEQNVTVGGKLHRLENPFMVLATQ
                     NPIEMEGTYPLPEAQVDRFFFKLILEFPTHAELSRIAELTTAAPPGPLREIVDGPAIL
                     QMRQWVRDVPLAEVVKDYAVRIVLGTHPQRQQGNGAGMAQRFVLYGSSPRGLQSLILA
                     AKARALFAGRPNVSFDDIRAMLLPSLRHRLILNLEADAEGIGAERILQEIVTQVPEVP
                     AKSL"
     gene            2187..3155
                     /locus_tag="VF089_16500"
     CDS             2187..3155
                     /locus_tag="VF089_16500"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6175611.1"
                     /translation="MQFSTRLFSTQTAKEHNIIKPAQAAVPEGPAETFFDAEFLKKIE
                     RLRLVAKRLSWAGAKGEHAVSRKGFSLEFSDYRNYQPGDDLRYVDWNIYRRLERLLVK
                     VFTAEEEMNIYLLIDTSRSMAQGSPAKIDYARRVAAALGYIGLKNLDRVGGASFATAL
                     HRPLTLGRGRKQILRLFNFLGGLSCSGATDLRLSVRAFCRLFPQSGLVIVVSDLFDPA
                     GWRGALQELAMKKHQILVIHIIDEQESSPSASGDITLRDVEGGHERKIFLDTELVRRY
                     QQELRRYLGEIEAICASRQIDYLRTTTQIPFEDFVLKTLRQASSVN"
     gene            3160..>4299
                     /locus_tag="VF089_16505"
     CDS             3160..>4299
                     /locus_tag="VF089_16505"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX6175612.1"
                     /translation="MFWGFPQALLLLVGAVPLILLLHSLKPKGIKLDTSTLFLWERVL
                     NEQPLATRLGWLLRKNLLLILQIVAATMLIAALADPALRHFGALSGDTVVVMDLSASM
                     KARGSSGTRFEAARRELLSLIDGLVSHQKIMVVGAGAQPRLLQPFTADKRKLRQMARD
                     LTATDAPGRMKEAILFAHAFLKRDTPDRVVVISDGAFEGAEEYSRPAAHLRFIKIEGG
                     SNNIGIVGFQLRRHPERRSQVEVMVHVKNFTAKSARVPLTLAMGEKTIVKESVDLEPD
                     GRRILIYPLGSLPQGALVARLDVDDDFATDNRAYLALSDRSPVRLLYVGPGNPFLTNL
                     FRFFPHVQVNTAPEWNANAASSQYDIVIFDSVPAPQLAQGNFILIN"
BASE COUNT          995 a         1160 c         1241 g          903 t
ORIGIN      
        1 aggagtacgc agtcattcca ctcttcctga tttggggcgg tcttctctgg ttcgatgtcg
       61 atgtacggtt cagacctgat gttcatacgc cgtcacagca gggtctggcg cagaagctac
      121 gggaattctc ccgtgagctt caagaaaagg ccggcagcga aggactgcgt cgaagtctgc
      181 aggttggccg ggaacttgaa aaggcggcgc agaaaagtat cgaggcgaaa attgccgatg
      241 agcaattcaa gagcgaaatt tcaggaatga gcagcaaggt tgaagcgatg ggaaaatcga
      301 tggggaacga gccatcgttt gcgacctcgg aaagcacgca gaacctgaag gatctcaaag
      361 ctgagctgga agccgcccag gagttgctga agttgcctga cggcgccaaa ggagaacgcg
      421 gactgggaca ctcatcactg gaccggctga agacaatgcc gcagctcaac cggcaactcg
      481 atcagcaggg tcaaacaaac cgttcgccga gccaaaatga actgagatcg tttctcgact
      541 ccatggataa acaggtcacc ggcgaactgg accggcgcac actactggat gctcaacagt
      601 tcctcgatca gctggctaaa cagggacacg gtgacaaggc cgaaaccggc gcgcgggtgg
      661 ctggacgggg cgaggaggat ctaccgggtg aaggtgaaaa agcacagagc aacagcaacc
      721 ggccgggaag cgagccggga acgaaggaag aaattcagcc agcgccacag ttgccgccgg
      781 ccgccgcgac tcagctgaaa ggcatgatcg gtcagggcaa tagtagcggc gttggcctca
      841 aagggaagcc atcgccggga aagaccgaag tttcgcaaga ggaagtgatt gccacttacc
      901 agcgacaagc tgaacaggag ctcaacaccg agcgcgtccc cgaagcgctg aaggagacaa
      961 taagaagata tttcttgtct ttgggaaata aacagcgcag cgatgagccg ggaaagtagc
     1021 taacctatta gcagcgatgc cggcagtcgt tgagttgccg gtatcgcgca tgaaagagca
     1081 aggagtttaa tcgtggaatc cgttgcagag atcgagcggc gtatacagag cgcccgccgt
     1141 tccttcgatg aagtcaaggc cgagatggcg cgggtgatcg tcggccacga ggacttaatc
     1201 gaagatattt ttatcgcgct tgtttgcggc ggccattgtt tgctcgaagg cgtacccggc
     1261 ttgggcaaga cattgctggt gcgcagtctg ggtgagatcc taagtctgag tttttcacgc
     1321 attcagttca cgcccgatct gatgccggct gatatcaccg gcaccaacat gcttagcgac
     1381 gatccctccg gcagaaaagt ttttgagttt cagcgcgggc cggtattcgc tcatatcgtt
     1441 ctggccgacg agatcaatcg cgccactccc aagacccagt ctgctttatt ggaagctatg
     1501 caggagcaaa acgtcacggt gggcggcaaa ctgcatcgtc tggaaaatcc cttcatggtc
     1561 cttgcaacgc aaaatccaat cgagatggag ggaacttatc cgctccctga agcccaggta
     1621 gatcgcttct tcttcaagct tatacttgaa tttccgacgc atgcggaact tagccgcatc
     1681 gccgaactga ccaccgctgc gccgcctggt ccgctgcgcg agattgtcga tggcccggca
     1741 attttgcaaa tgcggcagtg ggtcagggac gtccctttgg ccgaggtagt aaaggattat
     1801 gctgtccgga ttgttctcgg tacccatccg caaagacagc aaggcaacgg cgctggcatg
     1861 gcgcaaagat tcgttctcta cggctccagc ccgcgagggc tgcagagctt gattctggca
     1921 gccaaggcgc gggcgctttt cgctggaagg cccaatgtct ctttcgatga cattcgcgcg
     1981 atgctgctgc cgagcctgcg ccaccgtctg attttaaact tggaagccga tgccgaagga
     2041 atcggcgccg aaaggattct ccaggagatc gtcactcagg tacccgaggt tccagccaaa
     2101 agcctatgaa ttccgaattt cgcgttggca attcccagaa tcgcttgaaa cggtcctgcg
     2161 catccataca gacttttctc tcgtcgatgc agttctctac ccggctattc tcaacccaga
     2221 cagcaaaaga acataacatt ataaaacccg ctcaggcggc ggttccggag ggacccgcag
     2281 agacgttttt cgacgcggaa tttctcaaaa aaatcgagcg gcttcggttg gtcgccaagc
     2341 ggctcagttg ggcgggcgcc aaaggcgagc acgccgtttc acgaaaaggc ttcagcttgg
     2401 aattttccga ctaccgcaac tatcaaccag gcgacgatct gcgctatgtc gattggaaca
     2461 tctaccggcg gcttgagcgg ctgttagtga aagtgtttac cgcagaagaa gagatgaata
     2521 tctacctctt gattgacacg agccgctcga tggcgcaggg atcgccggcg aaaatcgatt
     2581 atgccaggag agttgctgcc gcattgggtt acatcggcct aaagaatttg gatcgggttg
     2641 gcggcgcctc gtttgcgacc gcgttgcaca ggccgctgac cttaggcagg gggcgaaagc
     2701 agatcctgcg tctatttaat ttcctcggcg gcttgtcgtg cagcggcgca accgacttgc
     2761 gcctgtccgt gcgcgccttc tgtcgtctgt tccctcagtc cggcttggtg atcgtggtca
     2821 gtgatctgtt cgatcccgcc ggctggcgcg gcgcgctcca ggaactcgcg atgaaaaaac
     2881 accaaatttt agtgattcac atcattgacg aacaggaaag cagtcccagt gcttcgggcg
     2941 acatcacctt gcgcgatgtc gaaggcgggc atgagcgtaa gattttcctg gatacggaac
     3001 tggtgcgccg ttatcagcag gaactgcgcc gctatctcgg tgaaatcgaa gctatttgcg
     3061 ccagccgcca aatcgattac ctgcgcacca cgacgcaaat tccattcgaa gactttgttt
     3121 tgaaaacgct gcggcaagcg agcagcgtaa actaaaaaga tgttctgggg ttttccacag
     3181 gcactactgc ttcttgtcgg cgccgtcccg ctgattcttc tcctgcatag tcttaaaccc
     3241 aaagggatca agctcgatac ctccacgctt tttctctggg agcgtgtgct taatgagcag
     3301 ccgctggcga cccggctggg atggctgctg cgcaagaatc tgctgctgat tttacagatc
     3361 gttgccgcaa cgatgctcat cgccgcgctt gccgatccgg ctttgcgcca ttttggcgcg
     3421 ttatccggag acaccgtcgt ggtcatggac ctgagcgcga gtatgaaggc caggggcagc
     3481 tcagggaccc gtttcgaggc agcgcgacgc gagcttctat cactgatcga tggcttggtg
     3541 tcgcatcaga agatcatggt cgtcggcgcc ggcgcgcagc cgcggcttct tcaaccgttt
     3601 acggcagaca agagaaagct gcgccagatg gcgcgagatc taactgccac tgacgctccc
     3661 ggtcgaatga aagaagcgat tctttttgct catgcatttc tcaagcggga tacacccgat
     3721 cgggttgtgg tgatcagcga cggcgcgttc gagggcgccg aagagtatag ccgcccggcg
     3781 gcccatcttc gtttcatcaa aatcgaaggc ggaagtaaca acatcggcat tgtcggattc
     3841 caattgcgcc gccatcccga acgccggtca caagtcgaag tcatggtgca cgtgaagaac
     3901 tttaccgcaa aatcggcgcg tgtgccgctg acattggcga tgggcgaaaa gacgattgta
     3961 aaagaatccg tcgacctgga gcccgacggc cggcggatat tgatctatcc ccttggaagc
     4021 ttgccgcagg gagcgctggt cgctcgtctc gacgtcgatg acgactttgc caccgacaat
     4081 cgcgcttacc tggcgctcag cgatcgatcg cccgtgcgac ttctttatgt aggaccgggg
     4141 aatccgtttc tgacgaacct attccgcttt tttcctcatg tgcaagtcaa cacggcgcct
     4201 gaatggaacg ccaacgcagc atcgtctcag tacgacatcg taatctttga cagtgtgccg
     4261 gcgcctcagt tggcgcaagg aaactttatc ttgatcaac
//
LOCUS       DASUTG010000714         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1591358, whole genome shotgun sequence.
ACCESSION   DASUTG010000714 DASUTG010000000
VERSION     DASUTG010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591358"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VF089_16510"
     CDS             complement(<1..899)
                     /locus_tag="VF089_16510"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX6175613.1"
                     /translation="MKREQINGLTNTRTIIALVGCAFGLGLMVIGIGVGSSSKDRVTS
                     AVKEESAKKLPAWNIALGDLVILAREMGFNVQGGKEAPIDEAKVAGRIESQLQRLRDL
                     YRQETSKNSALMGGVVLQLSVSSSGEVTKVREITSRISDYDFRTAVIADASNWSFDQV
                     LSQESTITCPLLFVREGMDITTLIQWEKNLSLPGDRTARAKLNGDMERAPQITAVRTP
                     SVRQASSKESAVAPPPQRPQPKPQSIAEGALYQMKYTTVIREEPNYSAAAVARFTTGT
                     KVILVGRAGDWYEVRYDGGPRGFL"
     gene            1283..1507
                     /locus_tag="VF089_16515"
     CDS             1283..1507
                     /locus_tag="VF089_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175614.1"
                     /translation="MIQARALSYFIVTAALLVSTRLIGETAEISDAQIRQGVQVALQY
                     TLSDETGAGIIWWNFYFSVAPGQHSQSPGS"
     gene            1532..1801
                     /locus_tag="VF089_16520"
     CDS             1532..1801
                     /locus_tag="VF089_16520"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6175615.1"
                     /translation="MKKKLLVIWDGSQPHRSRLVKDFVLEYLPAYAPELNPVEYIWGY
                     WKHHELPNFCPKTFSDLSYHAIRVLKRMRRRPTLITAFWNQAELF"
     gene            2157..3137
                     /locus_tag="VF089_16525"
     CDS             2157..3137
                     /locus_tag="VF089_16525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6175616.1"
                     /translation="MEYVQFGTSEFKVSRLGLGCMSMSGVYGPADDNESIATLHEAFD
                     LGINFIDTSASYGSGHNHELIAKALKGRRERIVIHSKSGSPRAPDASGMRSGSTPEYL
                     TQNCEQSVKRLGIDRLDIFCMSRVDPNIPVEESVGAMARLVERGLARYVGLSEASASS
                     IRRARKVHPIVSLQMEYSLWSRDPEAGNIAACREFNMGFMAYSPLGRGFFAGAVHDVK
                     DMTDGDGRSNHPRFQPENLARNLKLLTRFEAFAQDKSATPAQLALAWLMAQGNDIIPI
                     PSNKTRRHLEENIRAIDIQLSKEDLARLDGIFPPGAAAGPRTKDMHRVNI"
     gene            complement(3332..4315)
                     /locus_tag="VF089_16530"
     CDS             complement(3332..4315)
                     /locus_tag="VF089_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175617.1"
                     /translation="MKQFVSALLLILFCTSTVFAADRMRIAYSSISGAYIGIWAAREA
                     GFFAQENLDDQIIFIPSATQLSQVIVAGDVDIASLGGGPIMAAAISGAEMKIIGNNVN
                     KMIFSIHTKPEVKSIQELRGKRVGVTRFGSSSDVSARYALRKHNLDPQKDVILLQLGS
                     MTSIFGALKSGAIEAALVSPPTQFLSESLGFKELVSVTDMDYAYPNPAMAVPGSLIRN
                     KPDSIDRFMRAYIRGVHRARTAREFAIKTLAKYTTVTDPVLLGKTYDFYVGKVVEPAP
                     YVNMTGLQNALDDLARSVPAAKTAKPEQFVDMRFLQRVEKSGLLKELYKNP"
     gene            4527..5510
                     /locus_tag="VF089_16535"
     CDS             4527..5510
                     /locus_tag="VF089_16535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175618.1"
                     /translation="MKIQLNIAVALAIATALPSNLAAQIKTRIAWTSFASNMAGTWVA
                     QEEGLFKKNGVEAELVHIPSTSRAIQTMLAGEIAFTYMDGRNSVQAGLKGIDVVILAG
                     VANRLVFSFMSRPDIKNFAQLRGKKIGITRLGSSTHSVTLWTMSKMGLRPEEYQLLQL
                     VDVPNILTAIMAGQIDAGALSPPTNFRARKAGLNELIDLTKEGPEYVSVAIGSTRAFV
                     KANEDLTRRLIRAYSEGVQLLKANKAVGIRAIQKYARIKDPDILEATYGEARSYIETV
                     PYASKKGLETIIGELASTEAKSKLAKPDDFLDTRFVSQLEKEGFYKSASAK"
     gene            5626..>6120
                     /locus_tag="VF089_16540"
     CDS             5626..>6120
                     /locus_tag="VF089_16540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175619.1"
                     /translation="MPKVKLPSGVDLYYESHGQGEPLILVPSTAYSGEVWKPSQMPLA
                     DSLNLILLDPRGCGRSSPMQEVFTIEQMAVDIVALMDHLEIPTAHLLGHSMGGRIALS
                     MTQNFPGRVKSLIMAASGSGPAARAGADCIPGLPHRLVLEMVELGFERFIYNEICESD
                     TFFTD"
BASE COUNT         1423 a         1609 c         1642 g         1446 t
ORIGIN      
        1 agaaaacctc tcggccctcc atcataacgg acttcatacc agtcgccggc tctccccacc
       61 aaaataactt tcgtgccggt agtaaagcga gcgactgcgg cagccgaata gttgggctcc
      121 tcccgaatca ccgtcgtata cttcatctga taaagagcgc cttcggcaat ggattgcggt
      181 ttcggctgcg gccgttgcgg aggaggcgcg acggcacttt ctttggatga agcttgccgc
      241 acactcgggg tccttaccgc cgtgatctgc ggggcccgct ccatatctcc gtttagcttg
      301 gctcgcgccg ttctatcacc tggtagtgat agattctttt cccactgaat taaggttgtt
      361 atatccatcc cctcgcgcac gaatagcagc ggacaagtaa tcgtggactc ttgggaaaga
      421 acctgatcaa aactccagtt ggaagcatca gcgatgacgg cggtgcgaaa atcgtaatcg
      481 ctaatacgcg acgtgatctc gcgcaccttg gttacttcgc ctgaagagct gacgctcaac
      541 tgaagaacaa ctccgcccat gagcgccgag ttttttgagg tctcctggcg atagagatct
      601 cgaaggcgtt gcaattggct ctcaattcta cccgcaacct tggcttcatc gatgggcgct
      661 tccttgccgc cttgaacatt gaatcccatc tcgcgagcga ggatgacaag atctccgagc
      721 gcgatgttcc aggctggaag ttttttcgcg ctctcttctt tcacagcact cgtgacccgg
      781 tccttggaac ttgagccgac tccaattcca atcaccatga gtcccagtcc aaatgcacat
      841 cccaccagag cgatgatagt tcgggtattt gtcagaccat taatctgttc gcgtttcata
      901 atcccgatgc atcctttcgc agccgcatgt gggccatccg tttgagcaag tctcataccg
      961 gaggcctgca aacctgactg taaatcaggc gaaatgagca tcggaagcaa actcctcggc
     1021 agctttcagg acaatcacga caaactgcgg cgaaattctg tcacaggtta ccgctgatga
     1081 tcttcagcgc gcggcttgag ggataggtct acgaacttag gacgcttgac gttcggacat
     1141 tttctgctgt ctacacggcg acatcgaaca tttgtgcttc gctgcgccag cttatttgcc
     1201 gcgctaagtg tggttgaatt ctctggttcc acacgtaggc tcaagcactc acacctttac
     1261 agtcctggag gttcgaaagc caatgattca agcacgcgct ttaagttatt tcatagtgac
     1321 tgcggcactt cttgtgagca ctcgtctcat cggcgaaact gcagagatca gcgatgcgca
     1381 aattcggcaa ggcgtgcagg ttgccctgca gtacacattg tccgatgaaa ccggcgccgg
     1441 gattatctgg tggaacttct acttttcagt tgcacccggg cagcattcgc agtccccagg
     1501 tagttgagtt tctcgccatc ttaagcgtca catcaaaaag aagttgttgg tgatttggga
     1561 cggctctcaa ccgcatcgta gccggctggt caaagacttt gtgcttgaat acctgccggc
     1621 ctatgcccct gagcttaacc ccgtggaata catctgggga tactggaagc accatgagct
     1681 tcccaacttc tgtcctaaga ctttcagcga tctgagttac catgccatca gggtgcttaa
     1741 gcgcatgcgc cgccgtccca cactgattac ggccttctgg aaccaagcgg aattgttcta
     1801 aactaaatgg ccactatatc atccgaatta atagttcccg tacgacggca gcgttcccta
     1861 gtggccaact agatcactct aagtgcctac ttgccggcag cggttctagt tcgtgacttt
     1921 ttacctgcgc tatagaaaca tgatccttga ctacggccag taccacaatg atatgaacgt
     1981 atgaaagtag cttgggatac aataacccat gtagaacttc taacgggagc ggctgctcgg
     2041 ccgttctaga tcataccaca gcctcgctat tcaagaagat tggttaaaag gacaagcaag
     2101 cgaagaccgt gacaattcga tggtagagaa gacgtgcaca atttggagga ggcttaatgg
     2161 agtacgtaca atttggcacg agcgaattca aagtttcgcg actgggactc gggtgcatga
     2221 gcatgtcggg ggtatacggg ccggcggatg ataacgaatc gattgctacc ctgcacgaag
     2281 ccttcgatct cggcatcaat ttcatcgata cctctgccag ctatggcagc ggccacaatc
     2341 atgagctcat cgccaaggct ctcaagggca ggcgcgagcg catcgtgatt cattcaaaat
     2401 ccggcagccc gcgcgcgccg gacgccagtg gcatgcgcag cggcagcacg cctgagtact
     2461 tgacacaaaa ttgcgagcag agcgtaaagc ggctcggtat cgatcgcttg gatattttct
     2521 gcatgtctcg ggtcgatccc aatatcccag tggaggagtc cgtaggcgct atggcgcgtc
     2581 tcgtggagcg cggtctggcc cgctacgtgg gattgtcgga agcgagtgcg agctcaatca
     2641 gacgagctcg caaagttcat ccgattgtct cgctgcaaat ggaatactca ttgtggtcgc
     2701 gtgatccgga agccggcaat atcgccgcgt gccgtgaatt taacatgggc ttcatggcct
     2761 actcaccgct gggacggggg tttttcgcgg gcgctgtcca cgatgtgaag gacatgacgg
     2821 atggcgacgg ccgcagcaat catccgcgct tccagccgga gaatttagca cgcaacctga
     2881 aactcctcac gcggttcgag gcttttgccc aagacaaatc tgcgactcca gcgcagctcg
     2941 ctctggcatg gctgatggcg cagggcaacg acatcattcc aatcccgagc aacaagacgc
     3001 gccgccatct ggaagaaaac atcagggcga tcgatatcca gttgagcaag gaagatctcg
     3061 cccgtctgga cgggattttc ccgccgggag ccgcggccgg gccgcgcacg aaagatatgc
     3121 accgggtgaa tatctgagga aggttcaagc tgttcaagat gttcaaatcg ttcaagccat
     3181 tggaatgcgc ttaagagcgg ctgtgtaacc gtgtcatctt cattgcaacg atcctctata
     3241 ggccggcatc aacaagaatg ggagcctcac gccatttccc tcactctgaa acgttttgat
     3301 cggattgaac tgcttgaacg ttttgaactc ttcaaggatt cttataaagc tctttgagca
     3361 gcccgctctt ttccactctt tggagaaacc gcatatcgac aaattgttcg ggtttggcgg
     3421 ttttcgccgc cggtaccgaa cgggctagat cgtccaaggc gttttgcagg ccggtcatgt
     3481 tgacgtaagg cgccggctcg accactttgc cgacgtaaaa gtcgtacgtt ttcccgagta
     3541 gcaccgggtc cgtgacggtg gtgtacttag ccagtgtttt gatggcaaac tcacgagcgg
     3601 ttctcgcgcg gtgtacacct cttatgtaag cgcgcatgaa gcggtcaatg gaatcaggtt
     3661 tgttgcggat caacgatcct ggcacagcca tcgctggatt cggatacgcg taatccatat
     3721 ccgtgacgct taccagttct ttaaaaccta agctctcgga aaggaattgt gtcggcggtg
     3781 agaccagggc tgcctcgata gctcctgact tcagagcccc gaagatcgag gtcatggaac
     3841 ccagctgaag aaggattacg tccttttgcg gatcaaggtt gtgttttcgc agtgcgtacc
     3901 gcgcagaaac gtcggagctc gaaccgaatc gcgtcacacc aacgcgtttg ccgcgtagtt
     3961 cttggatact tttcacttcg ggtttggtat gaatcgaaaa gatcatcttg ttcacgttat
     4021 taccgattat cttcatttcg gcgccgctga ttgcggccgc catgatcggg ccaccgccca
     4081 aggaagcgat gtccacgtca ccggcgacga tcacctgaga aagctgcgta gcgctcggaa
     4141 taaagataat ctgatcatcc agattctcct gcgcgaagaa acccgcttcg cgagcggccc
     4201 agatgccgat gtaggcaccg ctgatagagc tgtaggcgat gcgcattcta tcggcggcaa
     4261 ataccgtaga cgtacagaag aggatgagga gaagagcgct gacgaactgt ttcatactga
     4321 gctccttttt gggctggtat ccattgggcc tacactcgcc cgcttccgga atttggcgta
     4381 tcggatcata caacgatcag tgaagttgcg atactcgcta gagcgcgttt gtagccttcg
     4441 ctgatcgttg ttgcttggtc tcgacgatcg agaaggtcca tgatattgag ctgccattct
     4501 catcgaaaaa gcccgaggca ccgaacatga agatccaatt gaatatcgca gtagcattgg
     4561 cgatcgcaac agcgcttccc tcaaatctcg ccgcgcaaat aaagacgcgt attgcctgga
     4621 cttcgtttgc cagcaacatg gctggcacgt gggtagcgca ggaagaggga ctgttcaaaa
     4681 agaacggagt cgaagccgag ctggtgcata tcccttcgac gtcacgggcg attcagacca
     4741 tgctcgccgg cgagattgcg tttacttaca tggatggccg caattcggtg caggcgggat
     4801 tgaagggcat cgacgtcgtc attcttgccg gcgtggctaa tcggctggtg ttttccttta
     4861 tgtcgcggcc ggatattaag aactttgccc aactcagagg aaaaaagatc ggcattacgc
     4921 gcttgggatc ctcgacgcac agcgtgaccc tctggacaat gtcaaaaatg ggactccggc
     4981 cagaagagta tcagttactt cagctggtcg atgtgcctaa tatcctaacc gcgattatgg
     5041 ctggtcaaat cgacgccggc gctttgtcgc caccaacaaa ttttcgcgcg cgtaaggccg
     5101 ggctcaacga actgattgat cttaccaagg aggggccgga atacgtctcg gtggcgatcg
     5161 gttccacccg tgctttcgta aaggcgaatg aggatctgac gcggcgtctg atccgcgctt
     5221 atagtgaagg agtccagttg ctcaaagcca ataaagcggt cggcataaga gcgattcaaa
     5281 agtatgcgcg gatcaaagat ccggatattc tggaagcgac ttacggcgag gcgcggagct
     5341 acatcgagac ggttccgtat gcgagtaaaa aaggcctcga aaccatcatc ggtgagttgg
     5401 catcgaccga agccaagagc aagttagcga aacccgatga ttttttagat acgcggttcg
     5461 tcagtcaact tgagaaggaa ggcttctaca aatcagcgtc tgcaaagtag cgagtctcga
     5521 agctctatcg cactggaccc agaaaggcat cctaacttac cgcatcaata gtcgtgatcc
     5581 gagagctcat gtttataaac tcgttgactt gaaggactta agcttatgcc gaaggtaaaa
     5641 ctgccgagcg gtgttgatct ctactacgaa tctcacggtc aaggcgagcc gctgattttg
     5701 gtgccatcca ccgcttattc cggcgaagtt tggaagccgt cgcaaatgcc gctggcggat
     5761 tctttgaatt tgattctcct cgatccacgc ggctgtggtc gatccagtcc gatgcaagag
     5821 gtttttacaa tcgaacaaat ggccgtggac atcgtggcgt tgatggatca tctggaaatt
     5881 cccactgcgc accttttggg ccactccatg ggcggcagga tcgcgttgtc aatgacacag
     5941 aactttccgg gtcgggtgaa aagtctgatc atggctgcga gcggttcagg tccggcggcg
     6001 cgtgcgggtg ctgattgcat tccgggtctg ccgcatcggc tggttctcga aatggttgag
     6061 ctgggtttcg aaagatttat ctacaatgag atctgtgaat cggacacgtt ctttaccgac
//
LOCUS       DASUTG010000715         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_850883, whole genome shotgun sequence.
ACCESSION   DASUTG010000715 DASUTG010000000
VERSION     DASUTG010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850883"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VF089_16545"
     CDS             <1..848
                     /locus_tag="VF089_16545"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX6175620.1"
                     /translation="YIALALIIYLGTQVVSALRWTVLTRPLGIRTSYKDLASYYFIGM
                     FFNLFAPSTVGGDVSRVYYLAREQNKDRQQRASGSTIQAAVSVLMDRAIGMVVLIWLG
                     AVGLLLFPEYGIPPAIRALTLSLAAAFLIVGILLPLVRRVLPEDAHPWVTKLRVILTS
                     YRRGWRVIPQAITLSFMVHLVQAWMHVIMGLALHLSLPLSFCIIVYPLVGTFAALPIS
                     VNGFGLREGGYIFLLALIGINSEEGIAFGLLLFLIVAVDSLIGGVLFLVKKESAPAGA
                     VSAEI"
     gene            complement(845..1723)
                     /gene="mgtE"
                     /locus_tag="VF089_16550"
     CDS             complement(845..1723)
                     /gene="mgtE"
                     /locus_tag="VF089_16550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6175621.1"
                     /translation="MNAANSKVFFYLYVVDNENKLVGVVPIRNLVIAPPDRTLSEMMI
                     ADPLKGDVFMDQEEAARIVAKYELLALPVVDEGGRLEGIITVDDVIDIIKEESTEDMY
                     KMAGLAEEDRVFTPISRSVKMRLPWTMLNLLTTGLAASVVTIFQGTITEVVALVTFMP
                     IVAGVGGNGATQTATVIIRGIALGELEFSSAWKAIIKQVSVNVCLALAAGAVIALIAA
                     LWQGNPTLGFVLALAMILNLGLVAGLSGAVIPLLLKALKFDPALGSGIVVTGLTDAFG
                     FLSFLGLATLFIRYLT"
     gene            complement(1674..2198)
                     /locus_tag="VF089_16555"
     CDS             complement(1674..2198)
                     /locus_tag="VF089_16555"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175622.1"
                     /translation="MKSTPIDYDTVREMLRTATPDRILRFIGKSHPADIALLFKGLDP
                     TEVRQFFDILFSARRAAKTLTELPPELLPDILALIEDDKIARVIVRADPDDAVTIIDS
                     LTEERRESVLSLLDPKRREEVRELISYPESTVGRIMNTEFMALPPTTTAQGAIDKIRE
                     RGELESFFLSLRRR"
     gene            2345..3781
                     /locus_tag="VF089_16560"
     CDS             2345..3781
                     /locus_tag="VF089_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6175623.1"
                     /translation="MSPIKSIYRLCFGLILGYSVAAIAAQDCWLSSAAAASSAVLENR
                     PPSFAAIAKRTMPIVVNISTTAQRAPRGSSADPIDEFFNRFFGESQPRDNSQRSLGSG
                     ILISKDGEILTSYHVVKNADAIKVKLADQSEYEARLVGKDDRTDLALIKIRKTTVPLP
                     FARLGTSSQLDVGDWVMAIGNPFGLEHTVTAGIVSAKGRVIGAGPYDNFIQTDASINP
                     GNSGGPLINAVGQVVGVNSAIFSQTGGNVGIGFAIPIDLAKKITDQLRKNGKVVRGWL
                     GIRAQAVSQQAASSLGLSHFVGEIQTVTEVTENSPAADAGIKVGDVIAEFNNKPLAKS
                     PDLRTMIADTAPGQKITLKIIREKLERIVSVRIGELPDDGDGSQQVEVRDPELGLRVQ
                     RITPETSRRLGLSSIKGVLVLEVQSGSPAEQVGLEPADVIREVNQKPVNNVKDFERAV
                     RQGRRGERILLLVQRGDNAVFFALKRKN"
     gene            3790..3987
                     /locus_tag="VF089_16565"
     CDS             3790..3987
                     /locus_tag="VF089_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6175624.1"
                     /translation="MFGLGMQELLIILAIALVVFGPSKLPQIGSGLGKAIRDFKKGVS
                     SDDSEDPVKEAKDENSKDFHR"
     gene            complement(4112..>4651)
                     /locus_tag="VF089_16570"
     CDS             complement(4112..>4651)
                     /locus_tag="VF089_16570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175625.1"
                     /translation="KVLEVGAGTGTSFPAYPTHCEVIGIDLAPDMLARAKQKIEDHGW
                     THLRVMEMNALNLDFPDNTFDYVMAFHVVTVVPDPIRMISEAKRVCKPGGSIVIVNHF
                     TSDVPVLGSVTEALDPLTRKLGWRTNLRLKPFIEAADLSVERAYKLSKTSLYTVVLGS
                     KSRNGRHFTDGAAVNNTHH"
BASE COUNT         1039 a         1206 c         1289 g         1117 t
ORIGIN      
        1 cctatatcgc cttggcattg atcatatatc ttgggaccca agtggttagc gctctgagat
       61 ggacggtgct gactcgcccg ttgggcatca ggacgtcgta caaggatttg gccagttact
      121 atttcatcgg catgtttttc aacctgttcg cgccgagcac agtgggcgga gatgtcagcc
      181 gggtctacta tcttgcgagg gagcagaata aagatcgcca gcagcgcgca tccggaagca
      241 ccattcaagc cgcggtctcg gtgttaatgg accgcgccat cggcatggtg gtgctcattt
      301 ggcttggcgc cgtcggtctt ctcctattcc ctgaatatgg aatccctcct gcgattcgcg
      361 cgctgacctt atccttggct gccgcgtttt tgatcgttgg catcctcctc ccgctggttc
      421 ggcgcgtgct gcccgaggac gcgcatccgt gggttacgaa gttgcgcgtt attttgacga
      481 gttaccgcag gggctggcgc gtcattcccc aagcgattac tctctcgttc atggttcacc
      541 tggttcaagc ctggatgcat gtgatcatgg gcttggccct tcatctgagt ctgccgcttt
      601 ccttttgtat tattgtttat ccattggtgg gaacctttgc ggcgctgccg atcagcgtta
      661 acggttttgg cttgcgcgaa ggcggctata tctttttgct tgcattgatc gggatcaact
      721 ctgaagaggg catcgctttc ggcttattat tattcctcat cgttgcggtg gacagtttaa
      781 tcggcggagt cctctttctc gtcaagaaag aatccgcgcc agccggcgcg gtcagcgccg
      841 agatctaagt cagataacga atgaacaatg tcgctaagcc gagaaacgac agaaagccaa
      901 aggcatcggt aaggcccgtt acaactatcc cggaacccaa cgccggatcg aattttagtg
      961 cctttaacag cagcgggatg actgctcccg agagcccggc caccagtccc aaattcagta
     1021 tcatcgccaa ggcgagcacg aaccctagag ttgggtttcc ctgccataag gccgcgatca
     1081 aagcgatcac ggcgccggcc gctaaagcca gacaaacatt aacggaaacc tgtttgataa
     1141 tcgctttcca cgccgaagag aattcaagct cgccgagcgc gatgccacgg ataattaccg
     1201 tcgcggtttg agtcgcgccg ttgccgccga cgccggcgac gatcggcata aatgtaacca
     1261 gggcgacaac ttccgtgatc gtgccttgga agatcgtcac cactgacgct gccaatcccg
     1321 ttgtgagtaa attcagcatc gtccacggga gccgcatctt aaccgaccgg gagatcggcg
     1381 tgaaaactcg atcctcctcc gctaagccgg ccatcttgta catgtcttcg gtgctttcct
     1441 ctttgatgat atcgatgacg tcgtcgaccg tgatgatgcc ttccaatcga ccgccttcat
     1501 cgacgacagg taaggctaaa agctcgtatt tagcgacgat gcgcgccgct tcttcctggt
     1561 ccatgaagac atcgcctttt agcggatcgg cgatcatcat ttcgctcaat gtgcgatcgg
     1621 gaggagcaat gactaaattg cgaatcggca cgacaccgac caatttgttc tcgttatcga
     1681 cgacgtaaag ataaaaaaaa actttcgagt tcgccgcgtt cacggatttt atcaatggcg
     1741 ccttgagccg tggtggttgg cggcaaggcc ataaattccg tgttcatgat tctgccgacc
     1801 gtgctttccg gatagctgat cagctcgcgg acttcctccc ggcgcttggg atccagtaac
     1861 gacaaaacag attcacggcg ctcttccgtc aggctgtcaa taatggtcac ggcatcatcg
     1921 ggatcggcgc gcacgatgac acgggcaatt ttgtcatcct cgatgagtgc cagaatatcg
     1981 ggcaacaact cgggcggaag ttccgtgaga gttttggcgg ctcgacgagc ggagaaaagg
     2041 atgtcaaaaa attgtcgcac ctcggtcggg tcgagtccct tgaagaggag cgcaatgtcg
     2101 gcaggatgac ttttgccgat gaagcgcaat atgcgatccg gcgttgccgt acgcaacatt
     2161 tctcgaaccg tatcgtaatc gatgggcgtg gatttcatct tgtccttggt attgcgactg
     2221 cgcgtctctt cttaaccatt ggtctagcga tttgcaaatg attttaacca attggcgccc
     2281 ttatagggag gataaaaatc tgctatcgtg agcttcactt tgacctgcga gcgagagagc
     2341 aagtatgtcg ccgattaaat ctatctatcg gctatgcttt ggcctcatac tggggtattc
     2401 agtggccgcc attgcagcgc aagactgttg gctgtctagc gccgccgcgg ctagttccgc
     2461 ggtgctggaa aatcgccccc cgtcgtttgc cgccatagcc aaacggacca tgcctatcgt
     2521 cgtcaacatt tcgacgacag cacaacgcgc accgcgaggc agttctgccg atccgatcga
     2581 tgagttcttc aatcgttttt ttggtgaatc tcagcctcga gacaacagcc agaggagtct
     2641 cgggtccggc attctgatca gcaaggatgg cgagattttg accagttatc atgttgtcaa
     2701 gaacgccgac gcgatcaaag taaagctcgc cgaccaatct gaatacgagg cccgcctggt
     2761 tggcaaggat gatagaacgg atttggcttt gatcaagatt cgtaagacga cagttcctct
     2821 tccgttcgct cggctcggca cctcgagcca gttggatgtc ggtgattggg tgatggcgat
     2881 tggtaatccg ttcggcttgg agcatacggt gacggcgggg attgtcagcg ccaaaggccg
     2941 ggtgattggc gctgggccct acgacaattt cattcaaacc gacgcctcga tcaatcccgg
     3001 caacagcgga ggaccgctga tcaatgctgt tggccaagtg gtgggagtga acagcgctat
     3061 cttcagccag actggcggca atgtcgggat cggctttgcg attcccatcg acttggctaa
     3121 gaaaatcacg gatcaactcc gaaagaatgg caaagtggtg cgtggctggc ttggtatccg
     3181 ggcgcaagcc gtttcacagc aagcggcgtc ttcgttgggt ctgtcccatt ttgtcggcga
     3241 gatacaaaca gtaacggaag tgacggaaaa tagtccagcg gcagatgccg gcatcaaggt
     3301 tggtgatgta atcgctgagt tcaacaacaa gccgcttgcg aaaagtcccg accttcgcac
     3361 tatgatcgct gacactgcgc ccggccagaa gatcacgttg aagattatcc gcgagaagct
     3421 cgagcgaatc gtctccgttc gcattggcga gttgccggat gatggtgatg gcagccagca
     3481 ggtggaagtc cgtgacccag aactgggatt acgagttcaa cgtatcacgc cggaaacctc
     3541 gcgccgtttg ggactgagct cgataaaggg ggttctggtt ctcgaagtgc aatccggcag
     3601 ccctgcggag caggtaggct tggagcctgc ggatgtgatc cgcgaggtta atcaaaagcc
     3661 cgtcaacaat gttaaagatt tcgaacgtgc cgtgcggcag ggcaggcgtg gggagcgaat
     3721 cttgcttctt gttcaacgag gagataacgc tgtattcttc gctttgaagc gtaagaacta
     3781 ggagggctta tgtttggact gggtatgcaa gagcttttga tcattttggc aatcgcgctg
     3841 gttgtcttcg gacccagcaa gctgccgcag atcggcagcg gcttgggtaa agccattcgc
     3901 gacttcaaaa aaggcgtgag cagcgacgac agtgaggatc cggtaaagga agctaaggac
     3961 gaaaattcca aagactttca tcgttagcag cccggcaatg gcaattctgt tgttccgacc
     4021 ctcttactcc tgtcggttct tctagtggat atgcgaaatt cgccggctcg atagagttat
     4081 cgagcgggta tgcagaatac agctctcgat ctcagtggtg ggtgttattg actgcggcac
     4141 catcagtaaa atgccggccg tttcggcttt tactgcccaa tacaacagtg tagagggatg
     4201 ttttcgatag cttgtaggct cgttctacac tcaaatcggc cgcttcaata aacggtttta
     4261 gccgtaggtt agtgcgccat cctaatttgc gagtgagcgg atccaatgcc tccgtcaccg
     4321 agcccaatac cggtacatca ctggtaaaat ggttaacgat gacgatcgag ccgccaggct
     4381 tgcagacccg tttagcttca gagatcatcc gtatcggatc agggacgact gtgaccacat
     4441 ggaaggccat cacatagtcg aaagtgttat ccggaaaatc aaggttgagg gcgttcatct
     4501 ccatcactct gagatgggtc cagccatgat cctcgatctt ttgcttggcg cgggccaaca
     4561 tatccggcgc cagatcgatt ccgataactt cacaatgagt gggatacgct ggaaaagatg
     4621 tgccggtgcc ggcacccact tccagaactt t
//
LOCUS       DASUTG010000716         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_703909, whole genome shotgun sequence.
ACCESSION   DASUTG010000716 DASUTG010000000
VERSION     DASUTG010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703909"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..2444
                     /gene="acs"
                     /locus_tag="VF089_16575"
     CDS             498..2444
                     /gene="acs"
                     /locus_tag="VF089_16575"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6175626.1"
                     /translation="MADKAIEVLLDEKRNFSPPPEFKKAANFRNVSVYNSARRNPQAF
                     WAKAAKELHWFKPWKKVLEGKVPWAKWFIGGKLNASYNCLDRHVENGRKNKAAIIWEG
                     EPGDERVLTYRDVWREVNKFANVLKKLGVRKGDRVCLYMGMVPELVIAMHACNRIGAP
                     HSVVFGGFSAEALRGRINDAQAKVLITADGGYRRGSVVPLKKNVDEALSDTPSIEHVV
                     VVERVGPESGAHMKSGRDQWWHELMADAALWCEPEQVDSEDILYILYTSGTTGKPKGI
                     VHTTGGYLTGVNLVNNWVFDLKEDDVFWCAADIGWVTGHTHIVYGPMANGATQVMYEG
                     TPDWPDKDRLWRLVEKYGVTIFHTAPTAIRTFMRWGTEFPAKHNLKSLRLLGTVGEPI
                     NPEAWVWYHKYIGGDRCPVVDTWWQTETGHPLITPLPGITKLKPGSATLPFPGIEADV
                     VDENGKSVPKGGGGYLVLTKPWPAMMRTIYGDPDRYVQNYWSRFPGKYFAGDGCKRDK
                     DGYFWLLGRVDDVMNIAGHRISTMEVESALVDHQKVAEAAVIGKEHEIKGQAICAFVS
                     LKAGVNGGNSLMDELKSHVAKKIGPIARPDEILFAAELPKTRSGKIMRRLLRDIADGR
                     ALGDTTTLADPNVVAKLKEQYAEE"
     gene            2449..3582
                     /locus_tag="VF089_16580"
     CDS             2449..3582
                     /locus_tag="VF089_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175627.1"
                     /translation="MRVTRFGMNKVGLDYRAWMQARLLVPSWERVTVPEKRDDIKSLK
                     IGFSWTVTLCMALSLWLLPTQADAQQREKVRVALGSISVNTSVIPVGQQAGLFTKHGI
                     DLEPIYMGGGMNSLAAVTSNSVQFLAAGSTATISARVGGMDIIMLSVQSNQLEYGIYS
                     APEIKTVQDLKGKIVTGTRPGASADSALRLFLRKAGLEPGKDVVFISVGESQQGRLNA
                     LHRGSVSATALLPPFTSMAKQLGFHELADMRKMDIQYAGTAIAGMASYIKSHPLVVEN
                     FLKGYVESLHYFRTQREKTIDGIMKFLKMSDRARAEEGYDFYVDLMPVMPYATPDGVK
                     SVLQFLTASQPKAATAKPEDFYDMSFLKKIEGSGFTKSLAARR"
     gene            3622..4632
                     /locus_tag="VF089_16585"
     CDS             3622..4632
                     /locus_tag="VF089_16585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175628.1"
                     /translation="MRTRRSRHALTLSLIIGSALLFSSAGAQDKQLKKISWGVTSLSA
                     SNWIPWIAKDAKIYEKHGLDVELVLVKGSGQTSSAILGGSLFAAPVAVPQVMMANLGG
                     ADLVNIAHTVPGVQSKLLVKQEIKRPEDIKGKSIATSSLGSLGDFLFKYIIRKNGMDP
                     MRDVTWLSIGTPPERLQALASGRVDAADLSHPTDAQALRMGYRVLWDARKEVVYPSMS
                     VVTRRRTVQEDRETVMRMLKSHVEAIAYFKTHKDFSIKVLSKYLRTNDQELLEGSYEI
                     YNKDFISVPYPIMHGLEATYEYVAQTRPEIRSRKPEEFMDPSLMAELDKSGFIKKLYA
                     QK"
BASE COUNT         1188 a         1247 c         1429 g         1089 t
ORIGIN      
        1 caggtaagca atcgggagct cgcaaggtgg gtctgacgcg aaggttatat gaactcatgg
       61 tcgcgataag atttacggcc tgctggcgtc gtaagcagcg cctcacaatt cgatccggat
      121 ccgacaatcc tcgcgattgt gaagttggcg tcagcgagaa aggtagttca ttgcgtatcc
      181 gctcgactcc cggggtgaat ctaaactcga gtgagaagta actaacggcg ctgatatttt
      241 tagccctgcg ttgggttcat tcttgggata gctttgtgaa cggtctatgc gttggcaatg
      301 ctgtgcgatc attttctcgc gcgtcatcgt cgctgcaggc ggatgcaacc gacgcagtgc
      361 tcgaccttct tccacactct cggtcgttaa actcgattaa ggacctaact cgattgggct
      421 cttgccaagc tgcgggagct caaatagaat ccggtcgttt ttcacgcctg tcaaagtagg
      481 tctcaaggag gagaatcatg gcagacaagg ccatagaggt tctgttggat gaaaaacgga
      541 acttttcgcc accgccggaa tttaagaaag cagccaactt caggaatgtg agcgtctata
      601 attccgcgcg tcggaatccg caggcgtttt gggccaaagc cgccaaagag ctccactggt
      661 tcaagccatg gaagaaggtg ctggaaggga aagtgccgtg ggccaagtgg tttatcggcg
      721 gcaagcttaa cgcctcgtac aactgtctgg atcgccacgt tgagaacggt aggaaaaaca
      781 aagctgcgat catctgggag ggagaaccgg gggacgaacg ggttctgacc tatcgcgacg
      841 tctggcgtga agtcaacaaa ttcgccaacg ttctgaagaa gctcggcgtg cggaagggcg
      901 accgggtttg tctctatatg gggatggttc ccgaactggt aatcgcgatg cacgcctgta
      961 accgcatcgg cgcgccgcat agcgtcgtat tcggcggctt cagcgctgaa gcgctgcgcg
     1021 gtcgcattaa tgatgcccaa gcgaaggtgt tgatcacggc cgatggtggc taccgccggg
     1081 gcagcgttgt gccgctgaaa aagaatgtcg atgaagcgct gagcgatacg ccttccatcg
     1141 agcacgtggt ggtcgtggaa cgagttggac ccgagtccgg ggctcacatg aaaagcggcc
     1201 gcgatcaatg gtggcatgag ctgatggcgg atgcggctct gtggtgcgag cccgagcagg
     1261 tggacagcga ggacatactc tacatcctgt atacgagcgg caccaccggc aagccgaaag
     1321 gaatcgtcca caccaccggc ggttatctta ccggtgtgaa cctggtcaac aactgggtct
     1381 tcgatctaaa ggaggacgac gttttctggt gcgccgccga tatcggctgg gtcaccggac
     1441 acacccatat cgtctatggg cctatggcca atggcgcgac ccaggtcatg tatgagggta
     1501 ctcccgactg gccggataag gatcgtctct ggcgcttggt ggaaaagtac ggcgtgacaa
     1561 tttttcatac ggcgcccact gcgattcgca cattcatgcg ctggggaacg gagtttccgg
     1621 caaaacacaa tctcaagagc ttgaggctgc tcggcaccgt gggcgagccg atcaatcccg
     1681 aagcctgggt ttggtaccat aaatacatcg gcggtgatcg ctgtccggtg gtcgatacct
     1741 ggtggcaaac tgaaaccggt catccgttga ttacgccctt gcccgggatc acaaagttga
     1801 agcccggttc tgcgaccttg ccgtttcccg gaatcgaggc cgatgttgtc gacgaaaatg
     1861 gaaaatcagt tcccaaggga ggaggcggct atctcgttct gaccaaacct tggccggcga
     1921 tgatgcgcac catctatggc gatccggatc gttatgtaca gaactattgg agccgctttc
     1981 cgggaaaata cttcgccggc gatggttgca agcgcgataa agatgggtac ttttggctac
     2041 taggccgcgt cgacgatgtc atgaatatcg ccggccatcg gatcagcact atggaggtgg
     2101 aaagcgctct ggtggatcat caaaaagtgg ctgaagccgc cgtgatcggc aaagaacatg
     2161 agatcaaagg ccaggcgatc tgcgcttttg tctctttgaa agcgggtgtc aatggaggca
     2221 actcccttat ggatgaacta aagagccatg ttgcaaagaa aatcggaccg attgccagac
     2281 ccgatgagat tctttttgcc gcggaactgc caaagacccg tagcggtaag atcatgcgcc
     2341 gtttgctccg tgacattgcc gatggccgag cgttgggaga taccaccacc ttggcagatc
     2401 ccaacgtggt agcaaaattg aaagagcaat atgccgagga gtgaactaat gagggtgaca
     2461 cggttcggga tgaataaagt cggtctggat tatcgcgctt ggatgcaagc ccgtttactt
     2521 gttccttctt gggagagagt caccgtcccg gagaaaagag acgatataaa atcgctcaag
     2581 atcggtttct cttggactgt aaccttgtgc atggcgcttt ctctatggct gcttccaacc
     2641 caggccgatg cccagcagcg ggagaaggtg cgcgtcgctt taggttcgat ttcggtcaat
     2701 accagcgtca tccccgtcgg ccagcaagcc gggctcttta ccaaacacgg gattgacttg
     2761 gaacctattt acatgggcgg cggaatgaat tcgctggccg cggtcacttc caactcggtt
     2821 cagtttctcg ccgcaggttc aacggccacc atcagcgcgc gcgtgggcgg catggatatc
     2881 atcatgctgt cggtgcaatc gaatcaactt gaatatggta tctatagcgc tcctgagatc
     2941 aaaacagtgc aggatttgaa ggggaagatc gtcaccggaa cacggcccgg cgcctcagcc
     3001 gatagcgcgc tgcgtctctt tctgcgcaaa gctgggttgg agccgggcaa ggatgtggtg
     3061 tttatctcgg tcggagaaag ccagcaagga cgactgaacg cgctgcaccg cggatctgtg
     3121 tctgcgacgg cgcttttgcc gcctttcacc agcatggcca agcaactggg atttcacgaa
     3181 ctcgccgaca tgagaaagat ggatatccaa tatgccggaa ccgccatcgc cggcatggcg
     3241 agctacatca agagccaccc gttggtggtg gagaatttcc tcaaaggcta tgtcgagtcg
     3301 ttgcactact ttcgaactca gagagaaaag accatcgacg gcattatgaa atttctaaaa
     3361 atgagcgatc gcgcccgggc cgaagaagga tacgattttt acgtcgactt gatgccggtc
     3421 atgccctatg ccacccccga tggagtgaaa tcggtgctac aatttcttac cgccagtcaa
     3481 cccaaagccg ccacggcaaa accagaagat ttttatgata tgagctttct taagaagatc
     3541 gagggaagcg gcttcaccaa gtcattggct gcccggcggt gatcggccgt ctccctaagg
     3601 cttgaatcaa ggagaaattc catgagaact aggcgctctc gacacgcttt gacattgtcg
     3661 ttgataatcg gctcggcttt gctcttctcc tctgcgggcg cgcaagataa gcaattgaag
     3721 aaaattagct ggggagtcac gtcgttgtcc gcatccaatt ggattccatg gattgccaaa
     3781 gatgcgaaaa tttacgagaa gcacggactc gatgtcgagc tggttctagt caaaggttcg
     3841 ggccagacct cgagcgcgat tctcggcggc agtctctttg ccgcgccggt tgcggttccg
     3901 caagtgatga tggcaaacct cggcggcgct gatctggtaa acatcgctca tacggtgccc
     3961 ggcgtgcaga gcaagctgct ggtgaagcag gaaattaaac gacccgaaga tatcaaggga
     4021 aagagcatcg cgacgtcaag tttaggctcg ctaggcgatt ttcttttcaa gtacatcatc
     4081 cggaaaaacg gaatggatcc gatgcgcgac gtcacctggc tatcgattgg gacgccgccg
     4141 gagcggcttc aagcactggc gtcgggacgg gtggatgccg cggacctgtc ccatcccacc
     4201 gatgcccagg cgctgcgaat gggctatcga gttctgtggg atgcgcgaaa agaggtggtc
     4261 tatccttcca tgtcggttgt gacccggcgc aggacggtac aagaagatcg cgaaacggtg
     4321 atgcgcatgc tcaaatccca tgtcgaggcg atcgcctatt tcaagacgca caaggacttc
     4381 agcatcaaag ttttgagcaa gtatctcaga accaacgatc aggagctttt ggagggttcg
     4441 tacgaaattt acaataaaga tttcatctcg gttccttacc ccatcatgca cgggctcgaa
     4501 gctacgtacg agtatgtagc gcaaaccagg ccggagattc gcagccgcaa acccgaggag
     4561 ttcatggacc cgagcctcat ggctgagctg gacaagagcg ggttcataaa gaagctctat
     4621 gcgcagaagt gatcgttagg catagcgata gtgaagacta aacgggagcg gctgtcgttc
     4681 gaaaaagcgg cgcaactgat aactgtttat ctctcgatca gcaaaatttc agaccattac
     4741 ccgccgaaga ttgcattatc tgctgcgcga atgatcttat cgccgcctta gcttgacagc
     4801 ctttttgctt tcaactacag ctctccaaag atttctctcg tttgacgagc tgatgcaaaa
     4861 tcagacgatg cgcaggaaaa tcttcgataa cggttcttgc ggtatcttaa gaacaaacca
     4921 gaggatgagg aggactgcga tgattgtgac acg
//
LOCUS       DASUTG010000717         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1444941, whole genome shotgun sequence.
ACCESSION   DASUTG010000717 DASUTG010000000
VERSION     DASUTG010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444941"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF089_16590"
     CDS             <1..442
                     /locus_tag="VF089_16590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175629.1"
                     /translation="EEEWDRVIGTNLVGAFLSSKAVASKLIEQESGRIISISSGRAFQ
                     GAKDAAHYASSKAGIIGFSKALALELAPHRITVNVICPGITDTAQPRGHQSEEQIYAQ
                     AQKIPLGRIGQPEDLVGTAVFLASDAAAFITGQTIIVNGGSIMW"
     gene            467..1210
                     /locus_tag="VF089_16595"
     CDS             467..1210
                     /locus_tag="VF089_16595"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175630.1"
                     /translation="MANKVAIVTGGAGGIGKAVVSRLAQAGFDVCIADQNKQAGQEVV
                     ELMRRQSCTAEFYQVDLKRAAEIKRLFDSIYAKFHGVDVLVNLAGGTLHKHAITEFPL
                     SQWREVIDVNLKATFLCCQAVSKAMRQAKSGVIINTSSNFGITGSAGRTAYSAAKAAI
                     IAFTKSLALELAPYGVRVNTIAPGLTATERVKAVHTDEEWLRLSAGMPLGRAGEPRDI
                     AEGVNFLAGDESAFITGQVLHVNGGMVFN"
     gene            1576..2604
                     /locus_tag="VF089_16600"
     CDS             1576..2604
                     /locus_tag="VF089_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175631.1"
                     /translation="MEITRLLIYRACKSVFGLLFFGVWFAWIGTTYSATMDLPKPEEE
                     LVWWTTMAQDQSQKVVEAFMQKHPSIRATYWRSGSNGMHNKIMTEARVGRHSWDVVTY
                     TAEFVLELKQKKLIAAYDSPERKNYSEDLKDDQGYWTAIYALPIGLGYNTELIKKEEV
                     PQSYNGLLESKKKGWKISIDNEGESLLIGLTKAWGREKAVQYLTKLAAQDVIPGRGNT
                     QRAQAVAAGEFPLLIAYTHSIEWFKLQGAPIDWVNLEPVVVQVNPVMLAAHAPHPTAG
                     KLFIDFMLSKQGQALLQSFHRVTTHTDVKPTPSRLIEGFKRVVLYPEDHQTLVESSKL
                     YKKIFGLQ"
     gene            2875..>3167
                     /locus_tag="VF089_16605"
     CDS             2875..>3167
                     /locus_tag="VF089_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175632.1"
                     /translation="MSKSKWGDRIPHIIEVRDDKFESPVERWAVNGEIQGGNVCNCPT
                     AMETRGYYPQRWEEVPLEVYDPASRLKALDRDGVDAEVLFPNGPGGTFLYDDQ"
BASE COUNT          794 a          768 c          911 g          694 t
ORIGIN      
        1 agaagaagaa tgggatcgag ttatcggaac caacttagtc ggtgcgtttc tctcttcaaa
       61 ggcggtggca agtaaattga tcgagcagga aagcggcagg atcatcagca tcagttcggg
      121 aagagcattt cagggagcta aggacgccgc ccactatgca tcttcgaagg cgggcatcat
      181 cggtttttcc aaggcgctgg ctttagagtt ggcgccgcat cggatcaccg tcaatgtcat
      241 ttgccctggt atcaccgata cggcccagcc gcgcgggcac cagagcgagg aacagatcta
      301 tgcccaagcc caaaaaattc ccctgggccg aatcggccag ccggaagatt tggttggcac
      361 ggctgttttt ttggcaagcg atgccgccgc cttcatcact gggcagacga tcatcgtcaa
      421 tggcggcagc atcatgtggt aagtcgagtt ggagaaagct gaaacaatgg caaacaaggt
      481 tgctatagtt acaggcggcg ccggaggcat cggcaaggcg gttgtatcac ggttggccca
      541 ggccggcttc gatgtctgta tcgccgatca gaacaagcaa gccgggcaag aagtggtcga
      601 gctcatgcgg cggcaaagct gcactgcgga attttatcag gtcgatttaa agcgagcggc
      661 agagatcaaa agactattcg atagcatcta tgcaaagttt cacggcgttg atgtgcttgt
      721 caatttggcc ggcggaactc ttcacaagca tgccatcacg gaatttccgt tatcgcagtg
      781 gcgcgaagtg atcgatgtca atctgaaagc gaccttcctc tgttgccagg cggtgagtaa
      841 agctatgcgg caagccaaaa gcggcgtcat catcaatact tcatccaatt tcggaattac
      901 tggcagcgcc ggaaggacag cttattcggc cgccaaagcg gccatcattg cttttactaa
      961 gtccctagcc ctggagctgg cgccgtacgg tgttcgcgtc aacacaattg cgcccggcct
     1021 gacggcgact gaacgggtga aagctgttca caccgacgag gaatggctgc gcttgagtgc
     1081 cggcatgccg ctgggccggg ctggagagcc ccgggatatc gccgaaggtg ttaatttctt
     1141 ggccggcgat gagagcgcct tcatcaccgg gcaagtgctc cacgtcaacg gcggcatggt
     1201 ctttaattaa gaatgtttcg tctgtagttg ctaggtcgaa tatcgttttg attcggagga
     1261 cacctgggtg aggtgaaaac ttttagtctc cgggtatata gacgatgcga gaactttcaa
     1321 gcgcacagcc aagccgacga gtaacatagc caggtgtttc tgccatttct tagcacgtac
     1381 gccacgggtg aagcggaaca gtggggcggt tgacgccgtc aaggaaacac taaacctaca
     1441 cgatcttgcg agaacggatg ccaggcgcac aaaactctcg ttgctatcga aacgtgggtt
     1501 ttcggaagtt ccggcttaac gtgtctcacg gatcgaacga atgaaagtgt cagtgtcaca
     1561 gaggagcatc atcgcatgga gataactagg ctgctcattt atcgcgcatg taaaagcgtg
     1621 ttcgggctgc tctttttcgg ggtatggttt gcctggattg ggacgacgta ctccgccact
     1681 atggatttac ccaagccaga agaagaactg gtgtggtgga cgactatggc gcaggaccag
     1741 tcacaaaagg ttgttgaagc gttcatgcaa aagcacccgt ccatcagggc gacttactgg
     1801 cggagcggat cgaatgggat gcacaacaag attatgacag aagcccgggt cggccggcat
     1861 tcgtgggatg tagtgacgta tactgcggag ttcgttttgg aactgaagca gaagaagctc
     1921 atagcagcct atgattcacc cgagcggaaa aactacagtg aggacttgaa ggacgaccaa
     1981 ggctactgga ctgcaatcta tgctcttcca atcggattag gctataacac tgaactgatc
     2041 aagaaagaag aagttccgca gagctataac ggtctccttg aaagcaagaa aaaggggtgg
     2101 aaaatctcca ttgataatga gggtgagagt cttttgattg gcctgacgaa ggcgtggggt
     2161 cgggagaaag ccgtccagta cctaacgaag ctcgcagctc aagatgtgat tccgggtcga
     2221 ggtaataccc agcgagcgca agcggtggcg gccggcgagt ttcctctgtt gatcgcctat
     2281 acacacagca tcgagtggtt caagttacag ggcgctccta tcgattgggt taacctggag
     2341 cctgtcgtag ttcaggtcaa cccggtcatg ttagcggcgc atgcgcctca tccgaccgcc
     2401 ggtaagcttt tcatcgattt tatgctttcg aagcaggggc aagctttgtt gcaaagtttc
     2461 catcgcgtca cgacccacac agatgtcaaa ccgactcctt cgcgattgat cgaaggtttc
     2521 aaacgtgtcg tgctctatcc ggaggatcat caaaccctgg tggagagttc caagctatac
     2581 aagaagatat ttgggctaca atagccaagt ccagcctccc aaggcgagat taattacgag
     2641 tctcgatctg acaatcagcg ttatactgac gctatgcgct tgtggtccat agaccatcaa
     2701 aatgctcaag tatcagcagc ttttttgcat gaggcggact ccgcaacgcg agaagatcac
     2761 gtcatcgacc gatcggctta atcactcggc aaaggacaaa gcatggagat aaaatacggc
     2821 ttgatcagtt ctgactctca tggccagctt ggcaaggatg cttacacaag caggatgtcc
     2881 aaaagcaagt ggggtgatcg aatcccccac atcatcgagg tacgcgacga caagttcgag
     2941 tcgccggtgg aacgctgggc agtcaacggt gagattcagg gcggcaatgt ttgcaattgt
     3001 cccactgcca tggaaacgcg cgggtactat ccgcaacgct gggaagaagt gccgttggaa
     3061 gtttacgacc cggcaagccg cttgaaagcg cttgaccggg atggcgtcga tgccgaggtg
     3121 ctgttcccaa atggaccggg gggaacgttc ctttacgatg atcagga
//
LOCUS       DASUTG010000718         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_850970, whole genome shotgun sequence.
ACCESSION   DASUTG010000718 DASUTG010000000
VERSION     DASUTG010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850970"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VF089_16610"
     CDS             <1..834
                     /locus_tag="VF089_16610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX6175633.1"
                     /translation="LFHDCCHGSFFGSRTANTILGYVSGIATFTPFEDWKYGHNSHHA
                     TAGDLDRRGIGDIWTMTREEYLAAAPRKRLRYRFYRNPFVLFGPGAALLFLFIQRFPT
                     KGAGKKERRSVLFTNLALLAIVALAASTIGFKTYLLIQLPIILIGGTFGLLLFYVQHQ
                     FENVYWARHDSWDPMRVALEGSSYLKLPKVLQWVTGNINLHHIHHVRPNIPNYNLQRC
                     YDEIPAFQEVKPITLWKSFSLLRLGLYDEAQQKMISFRSLKLSLLLARDEQYPRRAIP
                     T"
     gene            complement(1112..2281)
                     /locus_tag="VF089_16615"
     CDS             complement(1112..2281)
                     /locus_tag="VF089_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175634.1"
                     /translation="MQIPLKYILRSSSSRRLTTVITILGIALVVFVFTAVLMMANGVE
                     KTLRSTGSDDNMIVVRKAAMSEIMSILDRDAASIISNMPQVASFADGRPMSSKEAVVI
                     INLNKIGAPGISNVIVRGVEEAAFRLRPQVRIVQGRMFRWGAREVIVGAGITDRFAGA
                     QIGEQMKFGGDLWTVVGIFEADGSGFESEAWGDSNQIMDAFKRTSFSTVTMRLKQPDD
                     LSEIAAAFESDNRLQYFVAKREKKFFEEQSEMMAKFIRILGIFVTVIFSTGATIGAMI
                     TMYGAVANRTVEIGTLRALGFFRRSILLAFLGESFVLSLAGGIAGLGLASLLQFFSIS
                     TLNFGSFSELAFSFALSPSIIATSLGFSLLMGLIGGFLPALRASRLNIIQALRTA"
     gene            complement(2282..>3168)
                     /locus_tag="VF089_16620"
     CDS             complement(2282..>3168)
                     /locus_tag="VF089_16620"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6175635.1"
                     /translation="YIDKNQFFARLAVDSETFFDVYPEFVIEREQFDAFKRERNACII
                     GIDIARRYNLELGDIMLIEGDVYPGQWEFVVRGIYQPKDQTTDPASMMFHYKYLDERV
                     RQDMPQRSGEIGWYIVRIDDPDGSAAISQEIDQLFANSRAETKTETERAFQQSFLSAA
                     SAVITAMNIMSFVIIGIILLVLANTMVMSARERTHEFAVLKALGFSGGQLFFLIAGEA
                     LILSTLGSALGLLVTVPAVEGFQTALPKGWFPVFYIKPETIILGCVSAFVVGLASSLI
                     PLRRVLSTRIVEGLRHVG"
BASE COUNT          785 a          887 c          805 g          691 t
ORIGIN      
        1 ctcttccatg attgctgtca cggttctttc ttcgggtctc ggacggcaaa cacaattttg
       61 ggttatgtct caggcatagc gacgtttaca ccgtttgagg actggaagta tggccacaat
      121 tcgcaccatg ctacggctgg agacctcgac cgccgaggta tcggcgatat ctggacgatg
      181 acaagagagg agtatctggc agccgcccca cgaaaacgac tcaggtatcg attctatcgg
      241 aatcccttcg tcttgttcgg gccgggtgca gcgctcttgt tcttgttcat ccagcgattt
      301 cctaccaaag gcgccggaaa gaaggagcgc agaagcgtgc tcttcaccaa cctggcactg
      361 ctggcaattg tcgccctcgc cgcctccact attggtttca agacgtatct gctgattcag
      421 ttgccgatca ttctgattgg cggaaccttt ggattattgt tgttctacgt ccaacatcag
      481 tttgagaatg tgtattgggc acggcacgac tcttgggatc ctatgcgggt agccctagag
      541 ggtagttcgt atctgaagct accgaaggtt ctccaatggg ttacaggcaa catcaacttg
      601 catcacatcc accacgttcg acccaatatt cccaactaca atttgcaacg ctgctacgat
      661 gaaattcccg cgtttcaaga agtcaaaccc ataactctct ggaagagctt tagtttactg
      721 cgactagggc tgtatgatga agcgcaacaa aagatgataa gctttcgatc attgaagctc
      781 agccttttgc tagcccggga tgagcaatat cctcgacgcg ccattccgac ctgaccggta
      841 accgttggcc aaacgctctc ttccgagcca ctggaatatt ttcaactcgt cctaactaaa
      901 ctcgggacat caattttgcg agttggttca gacggctctc ttgccgcacc cgcatcttcg
      961 ggcccccgcc gtccgagatc agagctagat ctattcaggt cagttttgaa acgacagtca
     1021 taattcgggg gataaagctg cgccacagcg ttacccggag cggacgagga agatcggctt
     1081 tattgcaatc aaagcgaatg ttcgcatgtt cctacgccgt tcttaacgct tgaatgatat
     1141 tcaaccgcga cgctcgcagc gcgggcaaga agccgccgat cagacccatg agcagggaga
     1201 atccgagcga ggttgcgatg atcgaaggcg acagcgcgaa ggaaaacgcg agttccgaaa
     1261 acgaaccgaa gttcaaggtc gagatactga aaaactgcag taatgacgcc aaacccaatc
     1321 ccgctatacc gcctgccaaa gacaaaacga aagattcacc caaaaaggcc agcagaatgc
     1381 tgcgccgaaa aaaacctaaa gcccgcaacg tgccgatttc cacggttcga ttcgccacgg
     1441 cgccatacat cgtgatcatc gcgccgatgg tcgcgccagt gctgaaaatg acggtgacaa
     1501 aaataccgag gattcgtatg aacttcgcca tcatctcgga ttgttcttcg aaaaatttct
     1561 tctcacgttt ggcgacaaaa tattgcaacc gattgtccga ttcaaaggca gcggcaatct
     1621 ccgaaagatc gtcgggttgc ttaagtctca ttgtgaccgt cgaaaacgat gtgcgcttga
     1681 acgcatccat gatttgattg ctgtcacccc aagcctccga ttcaaatcca ctgccgtcgg
     1741 cctcgaagat ccccaccacg gtccagagat cgccgccgaa tttcatctgc tcgccaatct
     1801 gcgcaccggc gaatcgatcg gtgatgccgg cgccgacaat gacttcacgc gcgccccagc
     1861 gaaacatccg gccctggacg attctcacct ggggccgcag gcggaatgcc gcctcctcga
     1921 ccccgcggac aattacgttg ctgattccgg gcgcgccgat cttgttgagg ttgatgatca
     1981 cgacggcttc tttggagctc atcggccgcc cgtcggcaaa gctggccacc tggggcatgt
     2041 tactgatgat actcgccgcg tctcgatcga gaatgctcat gatctcgctc atggccgcct
     2101 tgcgcaccac aatcatgtta tcgtccgaac cggtggaacg aagcgtcttc tcgacgccat
     2161 tggccatcat caagacggcg gtaaaaacga aaaccaccaa ggcaatgccg agtatcgtga
     2221 tcaccgtagt caagcggcgc gatgaagaac tgcgcagaat gtatttaagc ggaatttgca
     2281 tctagccgac gtgcctgaga ccttcgacga tgcgcgtgct caacacccgc cgcaaaggta
     2341 tcagtgagga cgcgaggccc actacaaaag ccgaaacaca accgaggatg atggtctccg
     2401 gcttgatata aaaaaccggg aaccaacctt tgggcagcgc cgtttgaaat ccctccactg
     2461 ccggcaccgt gaccagcagt cccagggcgc tcccgagcgt agagagaatc aaagcttcgc
     2521 cggcaatgag gaaaaacaac tgcccgccgg agaagccaag cgctttgagc acggcgaatt
     2581 cgtgcgtgcg ctcgcgcgcc gacatgacca tagtattggc caggaccagc aaaatgattc
     2641 cgatgatgac aaaagacatg atattcatgg cagtaataac tgcgctcgct gccgataaaa
     2701 aactctgttg aaaggctcgc tcggtctcgg tcttggtctc ggcgcgcgaa ttggcaaata
     2761 actgatcgat ctcttgcgag atcgccgcag agccgtccgg atcatcgatg cgcacgatgt
     2821 accaaccgat ctctcccgac cgctgcggca tgtcctggcg cactcgttcg tccaggtatt
     2881 tatagtgaaa catcatgctg gcgggatcgg tggtctgatc cttgggctgg taaatgccgc
     2941 ggacgacgaa ctcccactgc ccgggataga catctccctc gatgagcata atgtcgccga
     3001 gctcgagatt atagcgccgc gcgatatcga tgccgatgat gcaggcattg cgctctctct
     3061 tgaaggcgtc gaattgttca cgttcgataa cgaattcggg atacacatca aaaaaggttt
     3121 cggagtccac cgccaaacga gcaaaaaatt ggtttttatc gatataga
//
LOCUS       DASUTG010000719         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1740963, whole genome shotgun sequence.
ACCESSION   DASUTG010000719 DASUTG010000000
VERSION     DASUTG010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740963"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1505)
                     /locus_tag="VF089_16625"
     CDS             complement(240..1505)
                     /locus_tag="VF089_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175636.1"
                     /translation="MSQDAMNRLVAALKYGAGLRDEFIYYMTFDDYKTLPDPFARTAF
                     AVKEVQQTIHRYANPEKRGELFRWDIHGRLFTPAKASIGNIAVVMIHGGAANEYEFIF
                     TPDGPEEYPDLSKTDPARARVGVAQHIASLGVPVLALSLPGHYSRKPWLPIPERRPEF
                     VIGEVPGDTELRNRLAVYTFRMCTEAIKTLVEKSLPDQDIFMWGHSTGGEYFYLMEQY
                     GLKNELIGGLGFGTGMPAWVRKEWDLACAEKSAEERAAPFRNLTDLSRRSPGSYIKSG
                     YVGPNQPWGSAEKWFELENHRRPQFKPFLQDIEHSAHDILMPEIRRISGLPDEELFIT
                     YKSDLTRLRGKKMLHVVGEYDKGHWIEGGEKGLEFRREVYAFKRFAPYAKELRLVQVP
                     NLTHYGHVESHNERLANIMVTGFKEYFLD"
     gene            1682..2776
                     /locus_tag="VF089_16630"
     CDS             1682..2776
                     /locus_tag="VF089_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6175637.1"
                     /translation="MSGDSQKASYEKVDRKAEVMDTPYDRWIASQGIDVIRGFFVDDL
                     YTIPLKWWDRIGGNGVYIMLDGTGYLDDAYVCGIPPGRSLNPQRHIYEALIYVLDGRG
                     ATTVWQTNGGKQSFEWQTGSLFAIPLNAWYQHFNGQGDREARLLAVTTAPLVFNLFHC
                     EDFVINNPYSFTDRFKGEEGFFGGKGTLYSDRVVETNFVADTINIEPVAWTERGKGNA
                     TIFFEMSDSMMGSHVSRFPVGIYKKAHRHGPGAHVFILTGRGYSLLWPEGKEMTRVDW
                     KPGSIVVPPEGWFHQHFNSSPEPARYLALKILSRKYKLQPGKIQSDVPLAFGGWQIEY
                     EDEDPIIRKIFEEECAKSGAEVRMPPIAPS"
     gene            2849..3676
                     /locus_tag="VF089_16635"
     CDS             2849..3676
                     /locus_tag="VF089_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175638.1"
                     /translation="MLKPIGLTEGHYECRSLDETLPIFTDLLALEIVERKDHEAVLKH
                     PNTAWRLVVHEGGPDAANKPHTNHYGFRVSSSKEIPKAYEYLTDHKDQYKIKSVTKPH
                     EAHFAYSIYFKEPGGNDLEIEYYNPAAVKQGRKIAAPHWTDPLPEEKFPGRGYVPQAM
                     THGTLNCDDKEVNDRFVKEVLGLEIIGGGRTSTYISHPSTPWYIVVLPTTRREYLTAA
                     NRFTLKLASAEEVADAHKEFSSKGKTSGINELWDLEENGRTHFIFSDISKNWWEVTS"
     gene            complement(3875..4873)
                     /locus_tag="VF089_16640"
     CDS             complement(3875..4873)
                     /locus_tag="VF089_16640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175639.1"
                     /translation="MSLKFSLHSLHGGVKSIDEIHQRAQRAEQLGYDGIFLGESHLSS
                     IDSFQTLASCAMLTQRVLLGIAVTNMVFRHPTVLAGAAASLNEISKGRAILGLGTGDG
                     PVYTQGLKATPMKQFEEGVKLIRHLVNGNSVRFPTGEFGISFTINRPAPIYVSAEGPK
                     GLQLAGRSADGVILGTGFDLHVFEWAKNKIREGTTAAGRDAKEVAIVAAGMLCVRDDG
                     NEARRIVRNRIANRAHHNFRFTLETVPAEELDGVKKFMAGFDVMKPMEDRVDPDLVND
                     YLVRRFSIAGTPTECIQRIEELKAAGVEHLMLTPARKVYHESVENFAKKIMPQFKN"
BASE COUNT         1157 a         1353 c         1371 g         1109 t
ORIGIN      
        1 acgattcgat cggcaaggtg accgcatcga gcgccactta gacaagaaag gcgaccgtat
       61 cgaggcttat tgggatcgcc gaggcaatcg tatcgatcac agattagatc ggcgtgacca
      121 ggaccacggc cgcttcgatc ataaccgcta tcgtcgttaa ccagaacgtt ggcacccagg
      181 cccgggaatt cgtcccggag cttggggtgc tccgttcgcc acaggttaca gcagcgtgtc
      241 taatctaaaa aatactcctt aaagcccgtc accataatat ttgccaatct ctcgttgtga
      301 ctttcgacgt gcccgtagtg cgtgaggttc gggacctgaa ccagccgcag ctccttggcg
      361 tacggagcga aacgtttgaa ggcataaacc tcccggcgaa actccaagcc tttctcgccg
      421 ccctcgatcc agtgaccttt atcgtactcg ccgaccacgt ggagcatttt cttgccgcgc
      481 agccgagtca gatcggattt gtaagtgatg aatagttcct cgtccggcag gccggagatc
      541 ctgcgaattt ccggcatcaa gatatcgtgc gcgctgtgct caatatcttg cagaaacggt
      601 ttgaactgcg gccggcgatg gttctccaac tcgaaccatt tttcggcgct gccccaaggt
      661 tgattaggtc cgacatagcc gctcttgatg tagcttccag gagaccggcg gctgagatcg
      721 gtgagattgc gaaacggcgc ggcgcgctct tccgcggatt tttcggcgca cgcgagatcc
      781 cactccttgc gcacccatgc cggcatgccg gtgccgaagc ccagcccgcc aatcagctca
      841 tttttaagtc cgtactgttc cattagataa aagtattcgc cgccggtgga atgaccccac
      901 atgaaaatat cttgatcggg caaacttttc tcgaccagtg tcttaattgc ctcggtgcac
      961 atgcgaaagg tgtaaacggc caggcgattt ctaagttctg tgtcgccggg aacttcacca
     1021 ataacaaact ccggccgccg ctctgggatc ggcaaccagg gtttgcggct gtaatgcccg
     1081 ggcagcgaga gcgccagcac cggcacaccc agtgaagcga tatgttgcgc aacgcctacc
     1141 cgcgctctgg ccgggtcggt cttgctcagg tcgggatatt cttcgggacc atcgggggta
     1201 aagataaact catactcgtt tgccgcgccg ccgtgaatca tgacgaccgc gatattgccg
     1261 atcgatgcct tggccggagt gaacagccgg ccgtggatgt cccaccgaaa gagctctccc
     1321 cgtttctcgg gattggcgta ccgatgaata gtctgctgca cttctttgac ggcaaacgca
     1381 gtccgcgcga aaggatcggg gagtgtctta taatcatcaa aggtcatgta gtagataaac
     1441 tcgtcgcgca aaccagcgcc atactttagc gcggcaacca gcctgttcat tgcgtcttgg
     1501 ctcattcatt cctccctgac tggcggactg tttcttgtga cctcgaccct tacctatctt
     1561 gcgaccggct gccgatgtca accgcatgcg gcccttggat tagcttgtca tgctgcgcgc
     1621 tgcttgatgg accgtggaga actaggataa agaacagtaa atacgccaag gaggctatca
     1681 gatgagtgga gattcccaga aggcttcata cgagaaagta gaccgtaaag ccgaggtgat
     1741 ggacaccccg tatgatcggt ggattgcgtc gcagggaatc gacgtgatta gagggttctt
     1801 cgtggacgac ctctatacca tcccgctgaa atggtgggac cgcatcggcg gcaacggggt
     1861 ttacatcatg ctcgacggca ccggctatct tgacgatgcc tatgtttgcg gcattccccc
     1921 gggtaggagc ctcaatccac agagacacat ttacgaagcc ctcatttacg ttctcgatgg
     1981 tcggggcgcg acgacggttt ggcaaaccaa tggcggcaaa caaagtttcg agtggcagac
     2041 cggcagcctt ttcgcgattc cgttaaacgc ctggtaccag catttcaacg gccagggtga
     2101 ccgcgaggcg cgattactgg cggtgaccac ggcgccgctg gtttttaatt tatttcactg
     2161 cgaagatttt gttatcaaca atccctattc cttcaccgac cgattcaagg gcgaagaggg
     2221 gtttttcggc ggcaaaggca cgctctatag cgaccgcgtc gttgagacca acttcgttgc
     2281 cgataccatc aatatcgaac cggtggcgtg gaccgaacgc ggcaagggga atgcaacgat
     2341 tttcttcgag atgtcagaca gcatgatggg gtcgcacgtt tcgcgctttc cagtgggcat
     2401 ttacaagaag gcccatcgtc atgggccggg ggcgcatgtg tttattctca ccgggagagg
     2461 ctactcactg ctatggccgg aaggcaagga gatgacccgg gttgattgga agccgggatc
     2521 gattgtcgtg ccgccggaag gctggttcca tcagcatttc aattccagtc cggagccggc
     2581 gcgatatcta gctctcaaaa tcctcagccg aaaatacaaa cttcagccgg gaaaaatcca
     2641 atccgatgtg ccgctggcgt tcggcggatg gcaaatcgag tatgaggatg aggatccgat
     2701 tatccgcaaa attttcgaag aggaatgcgc caagtcgggg gcagaagttc gaatgccgcc
     2761 catcgctcct agctgatgtt acacgaggct agggttttgc taagaatccg gtaaaataca
     2821 cgacaagttt gcactacagg agaaagtcat gttgaaaccc atcggattga cggaaggaca
     2881 ttatgagtgc cgctcgttgg atgaaacgct gccgattttc accgacttgt tagctctgga
     2941 aatagtcgaa cgcaaagacc acgaggcggt actgaagcat ccgaataccg cctggcgtct
     3001 ggttgtccat gaggggggac cggacgcggc taacaagccg cacaccaacc attacggatt
     3061 tcgggtttca agctcgaaag agatacccaa ggcctacgaa tacctcactg atcacaaaga
     3121 tcagtacaag atcaaaagcg tgaccaagcc tcacgaggct catttcgcct attcgattta
     3181 cttcaaggag ccgggcggta atgatctcga aatcgagtac tacaatccgg cggcggtcaa
     3241 gcagggccgt aagatcgctg cgccgcattg gactgacccg ttaccggaag agaaatttcc
     3301 cggcaggggg tacgtgccgc aggcgatgac tcacgggact ttgaattgcg atgacaagga
     3361 ggtcaatgac cgctttgtca aagaagttct cggactagag attatcggcg gcgggcgcac
     3421 ctctacctac atcagccatc caagcacgcc ctggtatatc gttgtgttgc cgaccacgcg
     3481 ccgtgaatat ttgaccgcag ctaatcggtt taccttgaaa cttgcgtccg ccgaagaggt
     3541 cgccgacgcg cacaaggaat tttcgagcaa aggcaagacg agcggtatca acgagctttg
     3601 ggacctggaa gaaaacggcc ggacccattt catcttcagc gacatcagca agaactggtg
     3661 ggaagtcacc tcttaaccgc gaacaaccgt tcaaaagttc aaggttcaac agttcaacat
     3721 aagattggaa tacatccgga tgacacgtaa caggtttcac ctcccatttt taactctgta
     3781 tcgaacagac ggaatcggca tatcgaagcc gaatcaatgc ggtgctgacc tggaaccact
     3841 gaactgttga accttaagtg cgcgcgacgc gcacttagtt tttgaactgc ggcatgattt
     3901 ttttggcgaa gttttcgacg ctctcatggt aaacctttcg cgctggggtg agcataaggt
     3961 gctcgacccc ggcggctttt aactcttcaa tgcgctggat gcattccgtc ggcgtgcccg
     4021 cgattgaaaa gcgccgaact aggtaatcgt tgacgagatc aggatcgacg cggtcctcca
     4081 tcggcttcat gacgtcaaag ccggccatga atttcttgac gccgtccagc tcttcggctg
     4141 ggactgtttc cagcgtaaag cgaaagttat gatgggcgcg gttggcgatg cgattgcgta
     4201 caatgcggcg tgcttcattg ccgtcgtcac gtacgcagag catgcccgcg gcaacgatgg
     4261 caacctcttt ggcgtcgcgc cctgctgccg tggttccttc acgaatttta tttttagccc
     4321 actcgaacac gtgcaagtcg aatccggtgc cgagaattac gccgtcggcg gagcggccgg
     4381 ctagctgcag tcctttggga ccctcggctg aaacatatat cggcgccggc cggttgatgg
     4441 tgaagctgat gccgaactca ccggtgggga accgaacgga atttccgttc acgagatgac
     4501 ggatcagctt cacgccttct tcgaactgtt tcatcggtgt ggctttcaac ccttgcgtgt
     4561 aaaccgggcc gtcgccggtg cccaatccga gtatcgcccg ccccttggat atttcattca
     4621 gcgatgccgc cgcgccggca aggaccgtag gatgacgaaa gaccatgttg gtgacggcaa
     4681 tgcctagaag aacgcgttgg gtcagcatcg cgcagctggc caacgtctgg aaagaatcga
     4741 tggagctcaa atggctttcg ccgagaaaga ttccgtcgta acctagctgt tcggcgcgtt
     4801 gcgcacgttg atgaatttca tcgatgcttt ttacgccacc atgaagactg tgcaaactga
     4861 atttaagcga catgtgcttc tccaacagca ggacatcagg cattacgcaa ttcgtacgtg
     4921 caaactagtc attcaatcct caggggtgag cccggagagg cgaacacgcc aaccgcacag
     4981 cctcaaccat
//
LOCUS       DASUTG010000720         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_851210, whole genome shotgun sequence.
ACCESSION   DASUTG010000720 DASUTG010000000
VERSION     DASUTG010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851210"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF089_16645"
     CDS             complement(<1..870)
                     /locus_tag="VF089_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175640.1"
                     /translation="MTSVHSPLFEPVLWEGDGFRILDEGSLPEQIRYISVSDVPQAIQ
                     AVREMKTRAFGQVLTFLYSGALLAQNYQKKETSPLRESIDQMSQEFCRARPTFDFHGL
                     GQYFDEWFEKLPRNIRAGEWISRRACDFAAQIIRARDARAKRAAELLPDQARVLTHCN
                     MSGELLAIALHCAAMGKDFAVIATETRPYLQGARLTAWELARGGVQVSLIPDCAVAQV
                     MARGQVDAVIVGSDRAAQNGDIINKVGTYPLALMAKQYGVPFHVLVQDPRSLVQGSDV
                     PIEERPGAELLSFQ"
     gene            complement(895..1629)
                     /locus_tag="VF089_16650"
     CDS             complement(895..1629)
                     /locus_tag="VF089_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175641.1"
                     /translation="MLKEAHFTPQRMLSMLGLKTDSLGPYALIPGPKERSQLLLGMLD
                     EPQKNFTFLDYEMHTGTLDGKRVTVGNGGRYAPDTAITTEILCGAGAQALIRVGSCGS
                     LQEHVKIGDLIIVTGALRGEGTSPYYVANNFSTVAHPEIVDALKRAAESLQVRFHQGW
                     IFTTDALFQETPELIAQLTQQNVSSIDMVTSVFLTIAQVRRKKAGAILAVSDECLHGK
                     FGFRDPSFLAAEERIIAVAREALKYL"
     gene            complement(1633..2172)
                     /locus_tag="VF089_16655"
     CDS             complement(1633..2172)
                     /locus_tag="VF089_16655"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6175642.1"
                     /translation="MHAYDLGFRREYERLQQKNPATITKARVRRLVIACGSCRRVWRE
                     HAKVAVAGLEVLHGVEYLEAALRMGQLKITKLIRKSYLSRFLSFGERFRHIPSAAQRL
                     GAIPGVQVVEMKRNQRSAWCCSGGVSEADPNLARWSATETGAQRVLTSSALSQRSFNA
                     LEQSALPTQELFEFVGQAV"
     gene            complement(2272..2508)
                     /locus_tag="VF089_16660"
     CDS             complement(2272..2508)
                     /locus_tag="VF089_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175643.1"
                     /translation="MFAVTVTAHADITVAMREQITEEEGPVDANRDLYQGLQQHDNPW
                     SQTSAEGGLWADELGSDEIGDRDAPTLLFARLLC"
     gene            complement(2922..3284)
                     /locus_tag="VF089_16665"
     CDS             complement(2922..3284)
                     /locus_tag="VF089_16665"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6175644.1"
                     /translation="MESDLGKVGLTPPQFYVLATIGYAGSLPFGEIGAKMMVTVSNLT
                     GIVDRLEQKNLVLRKRDETDRRVVHVTLTDKGANLYKTTIPLFERSISEIFSSLDLPR
                     QKELSALLRTLTHAHLAV"
     gene            3504..4238
                     /locus_tag="VF089_16670"
     CDS             3504..4238
                     /locus_tag="VF089_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6175645.1"
                     /translation="MKLRILGCGDAFGNGGRNQSGYLIESTDRLFLLDCGATSLLAMK
                     KLAIDSGQLDAIFLSHLHGDHFAGLPFFFIEYLYEKTREKPLHVAGPVGTEEKARGMF
                     DLMYGKGTKPGDLTTTSFHVLEPGRTTTIQGIEVSPFRVPHQVNEISLALKVSYNGKH
                     ILFSGDSAWTDEFLENARGVDVFICECTYYDRETSKHMSYLQLREQLPRLSCPRIILT
                     HMGSDMLAHSADVSYEMADDGMVVEI"
     gene            complement(4241..>4774)
                     /locus_tag="VF089_16675"
     CDS             complement(4241..>4774)
                     /locus_tag="VF089_16675"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6175646.1"
                     /translation="QKCYVGAVERNGVTLIVSLLGSRDLWGDTKRLLEYGFDNYHVLN
                     TLTPAAARPISAQRSHPDAAKLSALVAIPPEETASKPSSDGYVLQIGSFREQHRAEGL
                     LRAFSKKGFDAFIEKTTINDKGETSYRVRLGPYADLSEAQHILKEVTEESGYRAIVLP
                     YSIGETEAVGSQIEPPL"
BASE COUNT         1100 a         1292 c         1250 g         1132 t
ORIGIN      
        1 ttgaaagctc agcagctctg cgcccggcct ttcttcgatc ggcacatcgc tcccttgaac
       61 caaggagcgc ggatcttgaa ccaacacgtg aaacggcact ccgtattgct tggccatcag
      121 ggccagtgga taagtgccga ctttattgat gatatcgcca ttctgtgccg cacgatcgga
      181 accgactatc actgcatcga cctggcctct cgccatcacc tgtgcgaccg cgcaatcggg
      241 aatcagtgat acctgaacac ctcctcgtgc gagctcccac gcggtcagtc tggcgccttg
      301 caggtagggt cgggtttcgg tggcgatgac ggcgaagtct ttgcccatcg ccgcgcaatg
      361 gagcgctatt gccaatagtt ctccgctcat gttgcagtgg gtcagaaccc gcgcctgatc
      421 cggcaaaagc tcggcagccc gttttgctct tgcgtcccgg gctcggataa tctgcgcggc
      481 gaaatcacaa gctctccggc taatccattc accggctcta atatttctcg gcagcttttc
      541 gaaccactcg tcaaaatatt gaccaaggcc gtggaaatcg aacgtcggcc gtgcccggca
      601 gaactcctgg ctcatttgat cgatcgattc tcgcagcggg gatgtttcct ttttctgata
      661 gttttgcgcc aggagtgcac cgctataaag gaacgtgaga acttgaccaa acgcacgtgt
      721 tttcatttca cgtaccgctt ggatcgcttg cgggacatcg ctgacgctga tatatcggat
      781 ctgctctgga agcgaaccct catcgagaat tcgaaaaccg tccccttccc atagaaccgg
      841 ctcaaaaagc gggctgtgta ccgaggtcat agttaatctg cagttgcatc ggttctaaag
      901 gtacttgaga gcttcgcgcg caaccgcgat gattctctct tcagcggcca aaaacgacgg
      961 gtcacgaaag ccgaattttc cgtgtaaaca ctcatccgag acagcaagaa tggcgcccgc
     1021 tttttttctt cgaacttgag cgatcgtcaa aaaaaccgag gtcaccatgt cgatgctcga
     1081 gacattttgt tgtgtgagtt gggcgatcaa ctccggcgtt tcttgaaaca aagcgtcagt
     1141 ggtaaagatc cagccttgat ggaagcgcac ctgcaagctt tccgcggcgc gcttcagcgc
     1201 atcgactatt tcgggatgag ctacggtgga aaaattattc gccacgtaat agggcgaggt
     1261 gccttcgccg cgcagggccc cggttacaat gatcagatct ccaatcttca cgtgctcctg
     1321 caacgaaccg cagctcccga ctcggatcag cgcttgggca ccggcgccgc aaaggatttc
     1381 ggtggtaatc gccgtgtccg gagcgtagcg tccgccattg ccgacggtta ctctcttgcc
     1441 gtccagagtt cccgtgtgca tctcgtaatc caggaacgtg aaattttttt gcggttcatc
     1501 cagcatcccc aacagcaatt ggctccgttc cttggggccg ggaatcaagg cataaggacc
     1561 gagagaatcg gtcttcagcc cgagcatcga cagcattctc tgcggtgtaa agtgagcttc
     1621 tttgagcatc gatcaaacag cctggccgac aaactcgaat agttcttggg tcggcagagc
     1681 agactgctca agtgcgttga acgagcgctg ggacaacgcg ctggaggtca aaactcgttg
     1741 ggcgccggtt tccgttgcgc tccaacgagc gaggttcgga tcggcctcag aaacgccgcc
     1801 actacagcac catgccgacc gttgattccg tttcatctcc acaacctgga caccgggaat
     1861 ggcgcccaaa cgttgcgcgg cgcttggtat atgccgaaac cgttccccaa atgacaggaa
     1921 tcgtgataag taacttttac ggatcagttt ggtgatcttc aattgaccca tccgtaacgc
     1981 tgcttccagg tattccacgc cgtgtaagac ttccaaaccg gcgacagcaa ctttggcgtg
     2041 ctcgcgccaa acgcgccggc aactgccgca agcaattacc agcctccgca cccgggcctt
     2101 ggtgatcgtg gccgggtttt tttgttgcag acgctcatac tcgcgccgga atccaaggtc
     2161 ataagcatgc aacccgcagc attttcttga tcaccgagga tggcaaagtc ctcgcctgcc
     2221 ttttgcatca gcttggccaa ggccataacg ccggagcggc cgcttgcttg atcagcagag
     2281 cagccgggcg aacaacaacg ttggcgcatc tcggtcacca atctcatcag accccagttc
     2341 gtctgcccaa agtccgcctt cggcgctcgt ttgtgaccag ggattatcgt gttgttgtaa
     2401 tccttggtac aggtcgcgat tagcatcaac cggaccctcc tcctcagtga tctgctcgcg
     2461 catcgcgaca gtaatatccg catgggctgt aaccgtcact gcgaacaccg cacagctcct
     2521 ggcacatcag gcagattgta caactgtaga tgatttcctt gagttcccgc gtcatcgggt
     2581 cagggccgtg aaagacgcgc tgcgccatca accaacgcga cttgggggtg tggccttgga
     2641 agcgatataa ttcgtatgga gggcatttct ctttagggcc gggaacgtct tccatcgaat
     2701 tgaccggacc gtatccatag cacgaaccgc ataaaacgaa gccgctgctg cgcctcgaga
     2761 cccttggcat aaagccattt tttaacgcgc tcgagcccga gattcttttt tcgtgtcttg
     2821 cacaatttcg gccggtctga gatctgcaac agtttcgaaa atagatcctt aagacgttaa
     2881 ttgtatagct ccgaacaaac tggcgtgtca acaaactagc cctaaacggc gaggtgggcg
     2941 tgagtcagag ttcgaagtaa ggcggagagt tctttctggc gcggtagatc gagcgacgaa
     3001 aatatttcag agatacttct ctcaaagaga ggaattgtgg tcttgtacaa gttggcccct
     3061 ttgtccgtca gagttacgtg aacaacccgg cggtcggtct cgtcgcgctt tcgtaaaacc
     3121 aaattttttt gctccagccg gtcaacaata ccggtaagat tgctcaccgt gaccatcatc
     3181 ttggcgccga tctcgccgaa cggcaggctg ccggcgtaac cgatggtcgc gagcacgtag
     3241 aactgtgggg gtgttaaacc aactttgcca agatcagatt ccaacttttt atgcgatgag
     3301 agattaaacc taagaaaccg cacccagaga ctcgtgctgt aggaagcaaa aaagggacgt
     3361 ttctggatag gcacgtggtc aatttagtcg ccgtcaaatc aaaaagcaat aaaatagcta
     3421 cgaaggatcg atagctgtaa ccgtcccgcg gcttgactcc gggacgccag cggcttatct
     3481 ggaggataca ttcggagtaa gaaatgaagc ttcgtatatt gggctgcggt gatgcgttcg
     3541 gcaatggtgg ccgtaatcaa agtggatact taatcgagtc aacagaccgc ctgttcttgt
     3601 tagattgcgg agcgacttct ttgctggcga tgaagaagct cgcaattgat tccggccaac
     3661 tcgacgcgat ctttctgagc cacttgcatg gcgatcactt tgccggcttg ccttttttct
     3721 ttattgaata cttgtacgag aaaacccgcg aaaagccgct gcatgtggct gggccggtgg
     3781 gaacagaaga aaaagctcgt ggcatgttcg atctgatgta cggcaagggc accaagccgg
     3841 gagatcttac gactacctct ttccacgttt tagaacccgg gagaacgaca acgattcagg
     3901 gaattgaggt atccccattt cgagtgccgc atcaggtaaa cgaaatttca ctggcattga
     3961 aggttagcta taacggcaag cacattttgt tttcgggaga ctccgcctgg acggatgaat
     4021 ttctagagaa cgcccgcggg gtcgacgttt tcatctgtga gtgtacctac tacgatcggg
     4081 agacctcgaa gcacatgagc tatctacaat tgcgagaaca actgccaaga ttaagttgcc
     4141 ctcgaattat actgactcac atgggaagcg acatgcttgc tcatagcgcc gacgtttcct
     4201 atgaaatggc agacgatgga atggtcgtgg agatttgaag ctacagcggc ggctctatct
     4261 ggctgccgac cgcctcggtt tcgccgatcg agtaaggcag gacgatggcc cgatagccgc
     4321 tttcttctgt cacttccttg agtatgtgct gcgcctccga aagatcggcg taaggaccca
     4381 accggacacg ataagaagtc tcgcctttgt cgtttatcgt tgttttttcg ataaaggcat
     4441 caaaaccttt tttcgagaat gctctcaaca gaccttctgc cctatgctgc tcacgaaatg
     4501 aaccgatttg cagaacgtaa ccgtcgctgg agggtttgga agcggtctct tccgggggga
     4561 ttgctaccaa tgctgataat tttgccgcgt ccggatgact tcgctgcgca gatatcggcc
     4621 gagcagctgc gggcgttaat gtattgagga catggtaatt gtcgaatccg tattcgagca
     4681 gccgtttcgt atcgccccaa agatctcgcg agccaagtaa cgacactatc agggttacac
     4741 catttcgctc taccgcaccg acatagcatt tttg
//
LOCUS       DASUTG010000721         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_111558, whole genome shotgun sequence.
ACCESSION   DASUTG010000721 DASUTG010000000
VERSION     DASUTG010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111558"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VF089_16680"
     CDS             complement(<1..530)
                     /locus_tag="VF089_16680"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175647.1"
                     /translation="MTATFRSYLDALRNNSELIEITTATDARDIAALVPQAEQAILFR
                     KVTGYSMPVASGLLQSRNRLALAMGVPYEKIESKLRAAMEHPIAPQRVQRGAVKEVVR
                     TGRDINLFDLPVPVFSIMDGGPMITGGVVIGEDPEFGMNAGIYRLMLKERDITGIDIV
                     TPNNLRKFAERALQQQ"
     gene            648..1646
                     /locus_tag="VF089_16685"
     CDS             648..1646
                     /locus_tag="VF089_16685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175648.1"
                     /translation="MPSFGLTLANRGVIIGAVTVPELFDMAKRGEDSGAFDAVWVGDS
                     LLAKPRLESITLLSALAVVTKKVRLAVGCMATFPQRHPALLAQQWASLDVISAGRAWL
                     AVCLGGPNEQSPAQALEHRVMGIKDTERVGRLEEGIAILRKLFHEENASHKGRFYEFE
                     GVTIQPRPVQNPLPIWIASNPTGLTWKDGASAPSPAVERGLRRVAKFADGWMTNKVTP
                     QEFKSQWTRILEMAKEEGRDPSKIGSSLYHNINIKEDRQVALNESKAFLDKYYTSNFS
                     MNFVEGFTTAGTPQQCIEELKAYFAAGIEHITLRMTSWDQAGQMKRFLEDVAPAFK"
     gene            1668..2447
                     /locus_tag="VF089_16690"
     CDS             1668..2447
                     /locus_tag="VF089_16690"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175649.1"
                     /translation="MFKGIDHLVIVVKDLQQAAADYEKLGFKVVPGGKHPVGSHNMLI
                     AFSDGSYLELIGFYRDADDHRWWQPLQSGERLVDYCLQTDDLRGDTQKLRAAGVVIND
                     PVPWSRTRPDGYELKWILSLSTGSHRGFAPFLIQDVTPRNERIPQQFDHENGATGIDT
                     VMLAVDELTQVERWYSAVLGQSGKRVARNELGAEGLRFQIGPHFMEFLMPSDPSSPLA
                     DWLRMYGPSPYSATLRSQISDPRTLDQRLLHGAKIDLTGAK"
     gene            2472..3161
                     /locus_tag="VF089_16695"
     CDS             2472..3161
                     /locus_tag="VF089_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6175650.1"
                     /translation="MTTQETLALACRILAMHGHNDFIYGHVSALTDTPGQYWIKGSGI
                     GLEEVTEDDLVLIDFAGNKISGKRKRHNEFPIHSEVYRTNPEIRCVIHTHPVYSTIIA
                     SSEHRLLPITNMSCAFYPPALQKFDESSDLIVTPEQGIAVAGLLGEHRIVLLRNHGIV
                     VAANSIEEACVRGVLLEHSAKTQVVAASMGAFSWATDAEALLKRKRFYHPDAVHNLWE
                     YFGRQLRREQG"
BASE COUNT          737 a          919 c          881 g          713 t
ORIGIN      
        1 ttttgctgct gcagcgcccg ctcggcaaac ttgcgcagat tgttcggcgt gacgatgtcg
       61 atgccggtga tatctctttc ctttagcatc aagcgataga ttcccgcgtt catgccgaat
      121 tccgggtctt caccgatcac aacgccaccc gtgatcatcg gcccgccgtc catgatcgaa
      181 aacaccggca ccggcaggtc gaacaggttg atgtccctac cggtgcgaac cacttccttc
      241 acagctccac gctgaactcg ttggggcgca atcggatgct ccatggctgc gcgcagcttg
      301 ctttcgatct tttcgtacgg aactcccatc gccagcgcca accggttgcg tgactgcaag
      361 aggcccgagg ctaccggcat ggagtagcca gttactttcc gaaacaagat cgcctgctcg
      421 gcttgcggca ccaacgcggc aatgtcgcgc gcgtcggtag cggtggtgat ctcgatcaac
      481 tcgctgttgt tcctcaatgc atcaagatag cttctaaatg tggccgtcat ggatacctct
      541 ctcagattgc aggtaaccaa ccatagcgaa tttttgctgt caatcaccgc attggtttcg
      601 aaatgattag agcatatgct atgcaaagcg agaaggagaa tcggattatg ccctcatttg
      661 gactaacgct ggcgaatcgc ggcgtcatta tcggcgccgt tactgttccg gagcttttcg
      721 acatggcaaa acgcggcgag gactccggcg ccttcgatgc tgtctgggtc ggcgacagct
      781 tgctggcaaa accacggttg gaatcgatta cgctgttatc agctctcgcg gttgtcacaa
      841 aaaaagttcg tttagccgtc ggctgtatgg caacgtttcc gcagcgtcac ccggctttgc
      901 ttgcgcagca atgggctagc ctggatgtca tttcagccgg tcgcgcttgg ttggcagtgt
      961 gcttaggtgg gcctaacgag cagagcccgg cgcaggctct tgaacaccgg gtaatgggca
     1021 tcaaggacac tgagcgcgtt ggccgattag aagagggcat cgccatcctg cgcaagctct
     1081 tccatgagga aaacgcctcg cacaaagggc gcttctatga gttcgaaggc gtgacgattc
     1141 aaccacgccc ggtgcaaaac ccactgccga tctggattgc cagcaatccc accggcctga
     1201 cttggaagga cggcgccagc gcgccgtcgc ctgcggtgga acgcggttta cgccgcgtgg
     1261 caaaatttgc cgacggctgg atgaccaaca aagtcacacc ccaggaattt aaatctcagt
     1321 ggacccgaat cctcgaaatg gccaaggaag aaggccgcga tccgtccaag attggcagct
     1381 ccctttatca taacatcaac atcaaagaag atcggcaggt agcgctaaat gaaagcaaag
     1441 catttctcga caagtactat acgtcaaact tcagcatgaa cttcgtcgaa ggctttacca
     1501 ccgcaggcac tccgcaacag tgcatcgaag agcttaaagc ttatttcgcc gcgggtatcg
     1561 agcacattac cttgcgaatg acttcatggg accaggccgg acaaatgaag cgctttttag
     1621 aggatgtcgc gccggcattt aagtagcaaa gtaggcaatc gttagccatg ttcaaaggca
     1681 tcgatcattt agtcatcgta gtaaaagatc tgcagcaggc tgccgcggat tacgaaaagt
     1741 tgggctttaa ggttgtgccc ggcggaaaac atccggtcgg cagtcataac atgctgatcg
     1801 cgtttagcga tgggtcgtat ctcgaattga tcggattcta tcgtgacgcc gacgatcatc
     1861 gctggtggca gccgctacaa agcggcgagc gtttagtcga ttactgcctg caaaccgatg
     1921 atctgcgggg tgacacgcaa aagctgcggg ccgccggagt tgtcatcaat gacccggtgc
     1981 cctggtcacg tactcgcccc gatggctacg agctgaagtg gatactttcc ctgtcgaccg
     2041 gttcgcatcg cgggtttgcg ccgttcctaa ttcaagacgt cacgccgcgt aacgaacgca
     2101 ttccccaaca gtttgaccac gaaaacggcg ccactggaat cgacaccgtt atgctagcgg
     2161 tcgacgaact gacgcaagtt gagcgctggt acagcgcggt gctggggcag agcggcaaac
     2221 gagttgctcg caacgagctt ggcgctgagg gtttacgctt tcaaatcggg cctcatttta
     2281 tggagttcct gatgccctcg gatccgtcga gtccgctggc tgactggctg cgcatgtatg
     2341 gcccctcgcc ctactccgcc actttgcgca gccagatctc cgatccgagg accttggatc
     2401 agcgcctgct gcatggcgct aaaatcgatc taaccggggc aaagtagatt accgcagagg
     2461 agcagctgct gatgaccacg caagaaacac tggctttggc ctgccggatc ctggcgatgc
     2521 acgggcacaa cgattttatc tatggacatg tttcggcgct gacggatact cccggtcaat
     2581 actggatcaa ggggtccggg atagggttgg aggaagtaac cgaggacgat cttgtcctta
     2641 ttgactttgc aggcaataag atctcaggca agcgcaagcg gcacaatgaa tttccaattc
     2701 acagtgaggt ttaccggacc aaccctgaaa tccgctgcgt catccacacg cacccggtct
     2761 attccacaat tatcgcttct tcggaacacc gtctcctgcc gatcaccaac atgagctgcg
     2821 ctttttatcc accggcgttg caaaaatttg acgaatcttc cgacctcatc gtcacgcccg
     2881 agcaagggat tgccgttgcc ggattactgg gcgagcacag aatcgtgctg ctgcgaaacc
     2941 acggcattgt ggtagcggcc aattccattg aggaggcctg cgtccgcggc gtgctgcttg
     3001 aacacagcgc caagacccaa gtcgtcgcag catcgatggg cgctttttcc tgggccacgg
     3061 atgccgaagc cctgctcaag cgcaagcgat tttatcaccc cgatgcggtg cacaatctct
     3121 gggaatactt cggcaggcaa ctccgtcgcg aacaaggata gctcatcttc gcaatccgca
     3181 gaaacgcgtc cttttagtat tgaacccaat ccgttccgat ctcctacaat gcgcggcagc
     3241 tccaagagca
//
LOCUS       DASUTG010000722         5189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_704278, whole genome shotgun sequence.
ACCESSION   DASUTG010000722 DASUTG010000000
VERSION     DASUTG010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704278"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="VF089_16700"
     CDS             <1..1729
                     /locus_tag="VF089_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX6175651.1"
                     /translation="RVNEPDFNSINFDLIPIENIDRLEILPGTATVFGRNALGGVINI
                     TTKRGRRDRPHIGFDLGGGSYARQRYSLTSDGPLLPENVDYLLNVTRELSDGFRDGSG
                     ARITRLFGKAGYQIGDSTDATLSYTHVQDHLKQAGSLPGSFLRAGRRRHNLTPGDFAD
                     ANLDLLGLNLRQKLPAAFSLALNGFLRNHDQQIFVSGLSSTSNLDTDTVSAGGTVQIS
                     HEGTILGRKNVAASGFEYGHNRFETTSASAFICCPGDFISEQLTRENIVGVFFTDSLT
                     IVDSLIVNAGFRYDWDRLNFTGKTDPALSGTKEFKRFSPKTGVTYTPIDNLRLYFSYS
                     EGLRVPTVQEIFALGPFGSNLNLTPMKSRNFEVGFTSRPAEWLEAAISLFYMPVTDEI
                     LFIATDPLDPFSGRNENIRKTLRQGMEASLKLRPDKWIDAFINYTLTKATFEADFFIP
                     GINAVDPPRHVVKGDELPLVPRHRIGLGVNVRPLERLTLSLFGNYVGEQYQLRDEPNA
                     AKRLADYFVLNSRIAYERREWTAHLTLNNLTDRKYSTSGILVGAPFNESFRVPAPGIN
                     AFAGLSFRY"
     gene            1832..2797
                     /locus_tag="VF089_16705"
     CDS             1832..2797
                     /locus_tag="VF089_16705"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX6175652.1"
                     /translation="MQTHAESDLICLPFQRFRSTAAIIAIVLLLMGRSAVAAERIVLD
                     DLGFKVAVKHQVLRVVSLVPTNSEMVCLLACERLKGGTRYDEIPPELKRRVQQKQIEI
                     IGGGFDANLEKIVQLQPDLILTNGPSQQRFAVPLKNMGYPVLSLWPRDMITLRKDFLL
                     LGQLLEREHKAQQIMDQAQRNLLEIAASAKNHRRPRVYLQMWTEPLITVGRSSFPHWL
                     VATAGGINVFGDMPFDSGQISLEAIIERNPEVLIFLGDQKPFAERIKRRAGWSVIEAV
                     RNERFCFIEERDIRRSVQFAKGLRKIHDCLFDETKTAPRQAPEAN"
     gene            2794..3771
                     /locus_tag="VF089_16710"
     CDS             2794..3771
                     /locus_tag="VF089_16710"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6175653.1"
                     /translation="MKLKKPSWLLIGIAIFLSFVLGLSVGPVPIAFFEMSSTDRLILF
                     NLRLPRSLSAFFVGGGLALAGAIFQGLFRNPLADSYLLGVSSGASLGAASAVVLGLAS
                     PVAGFAAISFFAFLGSLMALGAVFYLGHRNGGFSIFRLLLSGLGVGLFLSSVVAIFML
                     MAGDELKSLIFFFLGGFASSSWDGTWVSLGLVPLAVIIACYFAPELNVLLLGEETASS
                     SGIRTSRIQLLYATVAALLTAISVSVAGLIGFVGLIVPHMIRLIFGADHRTLFPLTFF
                     SGGIFLLLCDTVARTIIAPAELPVGIITSLLGVPIFLYLIGKEPAGYTF"
     gene            3773..4606
                     /locus_tag="VF089_16715"
     CDS             3773..4606
                     /locus_tag="VF089_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175654.1"
                     /translation="MNRRMAIQAQELSFSYKDEPVLTNISFSIGAGQLVALIGPNGSG
                     KTTLLRLLLGHLRSGADQVWLDNRRLQTYAPKQLAKTVAYVSQQTGLNFPLTAFELVS
                     LGRFPHGSRFSFSRVDSLAVTRALEATHSVHLSERNFTTLSGGEQQKVLIARALAQST
                     SILLLDEPTVHLDLFHQLQILSALKRLCVEQQTTVLTVLHDINLVSLFADKALLLRSG
                     QLRACGPVAEVITERNIKELLGVDMKGAEDPETGVRFFIPRDPFRSTLNKPKPREAKE
                     H"
     gene            4614..>5189
                     /locus_tag="VF089_16720"
     CDS             4614..>5189
                     /locus_tag="VF089_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX6175655.1"
                     /translation="MAGKTLMVQGTGSSVGKSVLVTALCRIFKQQGLKVAPFKAQNMS
                     LNSFVTLDGGEIGRAQAVQAEASGIAASIDMNPVLLKPEADNCSQIIVGGKPLTRIRA
                     ADFPRLKSVLWAAITESLDRLRQRFDLVVIEGAGSPAEINLRDRDLVNMEVALYCDSP
                     VILAADIDRGGVFASLFGTLQLLQESEKEHIK"
BASE COUNT         1134 a         1398 c         1388 g         1269 t
ORIGIN      
        1 tcgcgtcaac gaacccgatt tcaacagcat caactttgat ttgattccga tcgagaatat
       61 cgatcgctta gaaattcttc cgggcaccgc aacagtgttc ggacgcaatg cgctaggtgg
      121 cgtcatcaac attaccacca agcgcgggcg ccgagaccgc ccgcatatcg gtttcgatct
      181 aggcggcgga agctacgctc ggcaaagata ttcattgact agcgacgggc ccctattgcc
      241 tgagaacgtt gattatctgc ttaacgttac ccgcgagctc agcgatggtt ttcgcgatgg
      301 cagcggcgcc agaattactc ggctatttgg caaggccggt taccaaatcg gcgactcgac
      361 cgacgcgacc ctctcctaca ctcacgtcca ggaccatctc aagcaagcag ggtcactgcc
      421 gggcagcttc ctgcgagccg gtcggcgtcg gcacaatttg accccgggcg actttgccga
      481 tgcgaatctt gatctgctcg gattgaacct ccgtcaaaag ttgcccgccg cattctcgct
      541 ggctctaaat gggtttctgc gcaatcacga tcagcaaatt ttcgtgagcg ggctttcgag
      601 cacttcaaat ctagacaccg acaccgtttc ggcgggcggc acggtgcaaa tcagccatga
      661 aggaactatc ctggggagaa agaatgttgc cgcaagcggc tttgagtacg gccacaatcg
      721 ttttgaaacc accagcgctt cggcgtttat ctgctgtcca ggcgacttca tcagcgagca
      781 attaacccga gagaacatcg tcggagtttt ttttaccgat agtttgacca ttgtcgattc
      841 tctgatcgtc aacgccgggt ttcgctatga ctgggaccgt ctgaatttta ccggtaagac
      901 cgatccggct ttgagcggaa ccaaggaatt caagcgcttt agccccaaga ccggtgtgac
      961 ctacactcca attgacaatc taaggttgta tttcagttac tcggaaggac ttagagtacc
     1021 caccgtccag gaaatcttcg cattgggccc ctttggctca aacctgaacc tgacgccgat
     1081 gaaaagccgc aacttcgagg tgggatttac gagcagacca gctgaatggc tggaagccgc
     1141 gatatcccta ttttacatgc cggtgaccga cgaaattctg tttatcgcca cggacccgct
     1201 ggacccattc tccggtcgaa acgaaaatat caggaaaacc ctgcgccaag gcatggaggc
     1261 ttcgctcaag ctccgcccgg ataaatggat cgatgcgttt atcaactaca cgttgacgaa
     1321 ggcaaccttt gaagcagatt tttttattcc gggcatcaac gctgtcgatc ctccgcggca
     1381 cgtcgtaaaa ggtgacgagc ttccgctagt gccacgtcat cggattgggc tgggtgtgaa
     1441 tgtccgtcct ctagaaaggc tgactctgtc gttgttcggc aattacgtgg gcgagcagta
     1501 ccaattgcgg gacgaaccca atgcggcaaa aagactggcc gattatttcg tgctgaacag
     1561 ccgcatcgcg tatgaacggc gcgagtggac ggcgcactta accctgaata accttaccga
     1621 cagaaaatac tcgacctctg gaattttggt tggagcgccg ttcaacgaat ctttccgtgt
     1681 tcccgcgccc gggatcaatg cctttgccgg tctgtcgttc cgttactgag tacgacttgc
     1741 gttaataaag gaattgactc cagatgagca cgacgtcgac atcgccactt tgcggtaatc
     1801 gattggcatg attccgcgca tccgagtaag catgcaaaca cacgccgaat cagacttgat
     1861 ttgcctgccg tttcaaagat tccgttcgac cgccgcaatc atcgccatcg tgttgttgtt
     1921 aatgggccgt tcggcggttg cggcagagcg aatcgtgctt gatgatctcg ggtttaaggt
     1981 ggcggtcaag caccaggtac taagggttgt atctttggtg ccgaccaact ccgagatggt
     2041 atgtttattg gcttgcgaga gattgaaagg cggcacccgg tacgacgaaa ttcccccaga
     2101 gctgaaaaga cgggttcagc aaaagcagat cgagataatc ggcggcggat tcgatgccaa
     2161 tcttgaaaaa atcgttcaac tgcagccgga tttgattcta acgaatgggc cgagccagca
     2221 gagatttgcc gtgccgctaa agaatatggg ctacccggtc ttgagtctct ggcctcgtga
     2281 tatgattact ctgcggaaag actttttgct gctcggccaa ctgctcgaac gcgagcacaa
     2341 ggcgcagcaa ataatggatc aggcgcaaag aaatcttctc gaaattgcgg ccagcgccaa
     2401 aaatcatcgg agaccacgcg tttacctgca gatgtggacc gagcccttga tcacagtggg
     2461 acgatcctct tttcctcact ggctcgttgc caccgcagga ggcattaacg tctttggcga
     2521 catgcctttt gattctgggc aaattagcct cgaggcgatt atcgagcgca atcccgaagt
     2581 cctgatattc ctcggcgatc agaagccgtt cgcggaaaga attaaaagac gagcgggctg
     2641 gagcgtgatc gaggcggtcc gaaacgagcg tttctgtttt atcgaagagc gggacatcag
     2701 acgcagtgtt caatttgcaa aagggctgag gaagattcac gactgcttgt tcgatgagac
     2761 aaaaaccgcg ccgcgccaag ctccggaggc aaattgaagc tgaagaaacc gagctggttg
     2821 ctgatcggca ttgctatatt tctctcgttc gtacttggcc tgagtgttgg accggtgccc
     2881 atcgctttct ttgaaatgtc ttcaaccgac cgtctgatct tgttcaactt gcggctgccc
     2941 cggagccttt cagctttttt cgtcggcgga ggattggcgc tggccggcgc catttttcaa
     3001 ggcctgtttc gaaatccctt agctgacagc tatctgctcg gtgtctcatc cggcgcgtcc
     3061 ctgggcgccg catccgcggt ggtgttggga cttgcttctc cagtagccgg tttcgcagct
     3121 atcagttttt tcgcattcct gggctcactt atggctctgg gagcggtctt ttacttgggg
     3181 caccgtaacg gcggcttctc gatctttcgc ctgcttctct ctggtttagg tgtcggtctc
     3241 tttctctctt cagtggtggc catcttcatg ctcatggccg gtgacgagtt gaagtcgcta
     3301 atcttcttct tcctgggcgg ctttgccagt tcgagttggg atggaacctg ggtttcacta
     3361 gggttggtgc cgctagcggt gatcatcgcc tgctatttcg ccccagagct gaacgttctg
     3421 ctattaggtg aggagacggc cagcagcagt ggaattcgga ccagccggat tcaattgctc
     3481 tacgccacgg ttgcagcgct gctaaccgcg atttcagttt cggtcgccgg attgatcggc
     3541 tttgtcggcc tgatcgtgcc tcatatgatc cggctcatct tcggcgccga tcatagaact
     3601 ttgttcccgt taaccttctt ttcggggggt atttttctgc tcctgtgcga caccgttgcg
     3661 cggacaatca tcgctccggc cgagctgccg gtcggcatta ttacttcgtt gctgggcgtg
     3721 ccgatctttc tttatctaat cggcaaagaa cccgccgggt acaccttcta agatgaatcg
     3781 cagaatggcc attcaggcgc aggagctttc ttttagctac aaagacgagc ccgttctaac
     3841 aaacatttcc ttctcgattg gcgcgggcca gctggtggcg ctgatcgggc cgaacggttc
     3901 cggaaaaacc actctacttc ggctccttct ggggcatcta cgatccggtg ctgatcaggt
     3961 atggctcgac aatcgccgat tgcagaccta tgcgccgaag cagctggcaa aaacagttgc
     4021 ctatgtttcg cagcaaaccg ggctcaattt tcccctgacc gcatttgaac ttgtatcgct
     4081 gggacgtttt ccgcacggct ctcgcttcag cttctcaaga gtggacagtt tggcagtgac
     4141 aagagcgctg gaggcgaccc actccgtgca tcttagcgag cgcaacttca cgactttgag
     4201 cggtggggaa cagcagaagg tcttgatcgc gcgagcgttg gcgcagtcca ccagcattct
     4261 tctgctggat gagccaacgg ttcacttgga tcttttccat cagctgcaga ttctgtctgc
     4321 cttgaagagg ctatgcgtcg agcagcagac cacggttctg accgttttac atgacatcaa
     4381 tctggtttct ctgtttgccg ataaggcact gctgcttcgc tcgggccagc tccgcgcctg
     4441 cgggcccgtt gcggaagtca ttactgagag aaacataaag gaactattgg gcgtagacat
     4501 gaagggcgct gaagatcccg aaaccggtgt acgcttcttc attccacgcg atccgttccg
     4561 ctctacgctc aacaaaccta aacctagaga agcaaaagaa cactagcact caaatggcag
     4621 gcaaaacgtt gatggttcaa gggaccggat catcggtggg caagagtgtt cttgtcaccg
     4681 ccctgtgccg gattttcaaa caacaaggac taaaggtcgc gccgttcaaa gcgcaaaaca
     4741 tgtcgctgaa ttccttcgtt actctggacg gcggtgagat cggccgtgca caggccgtgc
     4801 aagctgaggc ctccggaatt gccgcgagca tcgatatgaa tcctgttctg ctcaaacccg
     4861 aagccgacaa ctgctcgcaa attatcgttg gaggcaaacc gctgacaaga attcgcgctg
     4921 ctgatttccc ccggctcaaa tctgttttat gggcggcgat caccgaatcg cttgatcgcc
     4981 tgcggcagcg cttcgacctt gttgtcattg aaggagccgg aagcccggcc gaaatcaatc
     5041 tcagggatcg ggatctggtc aatatggaag tggcgctgta ttgcgactct cccgtcattc
     5101 tcgctgcgga tatcgaccgc ggtggtgttt tcgcctcatt gtttggaacc ctgcaattgc
     5161 tccaagaatc cgagaaagaa catatcaaa
//
LOCUS       DASUTG010000723         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1592532, whole genome shotgun sequence.
ACCESSION   DASUTG010000723 DASUTG010000000
VERSION     DASUTG010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592532"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1067)
                     /locus_tag="VF089_16725"
     CDS             complement(48..1067)
                     /locus_tag="VF089_16725"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175656.1"
                     /translation="MELRQAVKVLDSDAHARDLDENLRPYLDEPYRSQRAPFFLRETY
                     DRNLGGTLGFSGAKHEERLAAMDTQQIDSAVMYPTSGLGVGAIRDPAFNAALCRAYND
                     YISDYCKASPRLKAVANLPVNNPAEAAKELNRAVTKLELCGGMLAAQSHMKNLGSPEF
                     YPLYEEAQRLNTPIAIHAFGGDEPGTEIFDKFICLHTTGHPFPVLRQLTAIVFGGIPE
                     LFPNLKIGYLEVGCGWIPYWAERMDEEWEKRGKAEAPLCKKKPSEYLTNGNSYYGCEP
                     EEKMMGYVVNEIGSKTLMYASDYPHWDMSWPESATIVWNREDLSVEAKKDILLHNAKR
                     FYNLS"
     gene            complement(1095..2255)
                     /locus_tag="VF089_16730"
     CDS             complement(1095..2255)
                     /locus_tag="VF089_16730"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175657.1"
                     /translation="MEQTIISSDSHVIEVPDLWEKGVPSSFRERAPKAFFDEQRDAWM
                     FGSAEVPVQAVGGLFMAGQRPDQVESFRRAGFSVARPGGWDPFERMKDMVTDGVAAEV
                     LYPSLGLGLFCLEDAALQEALFRTYNDWLIDYCHKVPDRLFGIALISMFDIDHAIAEM
                     KRCKEQGIVGTMIWQVPHPRLPFASEHYERFWAASEDLGLPVHLHILTGFGDSMHRQT
                     SHGIKRYRIGVQQTREIEDALFDIIFTGVLERYPRLKVVSVENEIGWMPFWLGQCDKA
                     FKRHRHSEKLTIDELPSEYFRRQVYATFFNDPVGAKLFSWWGCDNFMWSNDYPHQNST
                     WPNSRDVIARDMGHLDAADREKLLSSNVIDLYQLKVAASLPETHVGAAAAHA"
     gene            complement(2271..3266)
                     /locus_tag="VF089_16735"
     CDS             complement(2271..3266)
                     /locus_tag="VF089_16735"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175658.1"
                     /translation="MTRKILAAIYFLLLAPALIHAASAPDKLVGIHSARVMSQSLPWM
                     AQEAGLFKKYNLDFNLVFISSSSIVTAALLGGDAEMTLTGGIGNVIAYVRGSTDVVFV
                     GGVKNVMTQSLVAGGSIKKPEDLKGKRIAVSRIGGNTHYFTIQALRRHGLEPNRDFSF
                     MQSGGDPEALAALMTNNVEAATLTPPSDAKALSSGFHYVLYGPELKIPYAATAFVTKR
                     SVIAKRPQVIGNFMRSMAEAAKIMHTDRDFVYKVLGKYLRVTEKSVLDAAYNAEIKAL
                     EPRLAIKTEAIQATLDEIAANDPRAKKISPEEIIDRRYLEEMEKSGFFEQLWAKK"
     gene            complement(3635..>4303)
                     /locus_tag="VF089_16740"
     CDS             complement(3635..>4303)
                     /locus_tag="VF089_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175659.1"
                     /translation="GLMVMLFKRLKFNEALLRQGGWKEPQNRGMLMMGRTIGIIGVGR
                     IGRNVVKRLSGWDLKFLGYDPYVDQETVAPLGIKMVSLEELLRESDLVTIHVVLTRET
                     RKMIQLPQLKMMKKTAYIVNTSRGQVFNEADLAQALNEGIIAGAALDVFDEEPTPMDN
                     PLRQVDPTKLTMTPHIIGNNPGSLEAGQRLAAQSILSILEGKPPDTVVNPKAIPLWKE
                     RFWS"
BASE COUNT          924 a         1240 c         1136 g         1003 t
ORIGIN      
        1 cgccggtccg caccagaaat gctcaagacc ggcggccgct ttaagcgcta cgagagattg
       61 taaaatcgct tggcgttatg aagcagaata tcctttttag cttccaccga caggtcctca
      121 cggttccaca caatggtcgc cgattcaggc caactcatat cccagtgcgg ataatcggag
      181 gcatacatca aagtcttcga gccgatctcg ttgacaacgt agcccatcat cttctcttcc
      241 ggctcgcaac cgtaatagct gttgccgttg gtcaaatact cgctcggttt tttcttgcac
      301 agcggagctt cggccttgcc gcgcttttcc cattcttcat ccatccgttc cgcccaatag
      361 ggtatccagc cgcaccccac ttcgaggtag ccgatcttca ggttgggaaa cagttcgggg
      421 atgccgccga agactatcgc ggtgagctgg cgtagcaccg gaaagggatg gccggtggtg
      481 tgcaggcaga tgaatttatc gaaaatctcg gtgccgggct catcgccgcc gaaggcgtga
      541 atcgcaatcg gcgtattgag ccgctgcgct tcttcataga gcgggtagaa ctcggggctg
      601 ccaagatttt tcatatggga ctgcgccgcc aacatgccgc cgcatagctc gagtttggtg
      661 accgcgcggt tgagctcctt ggccgcttcg gcgggattgt tgaccggtag attggccact
      721 gccttgaggc gaggcgacgc cttgcagtaa tccgaaatgt aatcgttata ggcccggcat
      781 agggctgcat taaacgccgg atcgcgaatc gcgccgacgc ccaagccgct ggtcggatac
      841 atgaccgcgc tatcaatttg ctgcgtgtcc atggctgcca gacgttcttc gtgtttcgcc
      901 cccgaaaagc ccaatgttcc gcctagattg cggtcgtagg tttctcgcag aaaaaacggc
      961 gctctctggc tgcggtaggg ttcgtccaga tagggccgga gattttcatc gagatcccgc
     1021 gcatgcgcgt cggaatctaa aacttttacc gcttgtctca attccataat cgctcctcta
     1081 tcttaaatgt taattcaggc gtgcgctgcc gccgcgccga catgggtctc gggcaacgaa
     1141 gccgctactt tcaattgata caagtcgatg acgttgctgc taagcagctt ctcccgatcg
     1201 gcagcatcga gatgacccat atcacgcgca atcacgtccc gcgagttggg ccaggtggaa
     1261 ttttgatgcg ggtagtcgtt ggaccacatg aagttatcgc aaccccacca tgaaaacagt
     1321 ttggcgccga ccggatcgtt gaagaatgtc gcataaacct ggcggcggaa gtattcgctt
     1381 ggcagctcat cgatcgtcag cttctcggaa tgtcgatggc gcttgaaggc tttgtcgcat
     1441 tggcccaacc agaacggcat ccagccgatt tcgttttcga ccgaaactac tttaagccga
     1501 ggatagcgtt ccagtactcc ggtgaaaatg atgtcgaaaa gagcgtcctc gatttcgcga
     1561 gtctgttgca cgccgatgcg gtagcgcttg atgccgtggc tggtctggcg atgcatgcta
     1621 tcgccaaagc cggtcagaat gtgcaggtgg accggcaagc ccagatcctc ggatgccgcc
     1681 caaaatcgtt cataatgctc ggaggcaaaa ggcagtctgg gatggggcac ttgccagatc
     1741 atcgtgccga caatcccctg ttccttgcag cgcttcattt cggcaatggc atgatcgata
     1801 tcgaacatcg aaatcagcgc gatgccaaag agccggtccg gcactttatg acagtaatcg
     1861 atcagccaat cgttataggt tctaaagagc gcctcctgaa gggcggcatc ttccaggcag
     1921 aacaaaccga gaccgagact gggataaagc acttcagcgg cgacgccgtc agtaaccatg
     1981 tccttcatcc gctcgaacgg atcccagccg cccggacggg cgaccgagaa accggcgcgg
     2041 cgaaaacttt ccacctgatc cggacgctgg ccggccatga acaagccgcc caccgcttgc
     2101 accggcactt cggccgagcc gaacatccag gcatcgcgct gctcatcgaa gaaagctttg
     2161 ggggcgcgtt ccctgaacga cgatggcaca cccttttccc ataggtccgg aacctcgatt
     2221 acatgagagt ccgacgaaat gatggtttgt tccatggctc ctcccttgtg ctatttctta
     2281 gcccataact gctcaaagaa tccgctcttt tccatttcct cgagatagcg gcgatcgatg
     2341 atctcttcgg gtgagatctt ttttgcccgc gggtcattcg cagcgatttc gtcgagagtc
     2401 gcttgaatcg cttcggtctt gatcgccagg cgcggctcca acgccttgat ctcggcattg
     2461 tacgcggcat cgagaactga tttttcagta acccgaagat acttgcccaa cactttgtaa
     2521 acaaaatcgc ggtcggtgtg catgattttg gcggcctctg ccattgaccg catgaaattt
     2581 cctattacct gaggccgttt ggcgatcacc gagcgctttg tcacaaacgc cgtcgcggcg
     2641 taagggattt tcaattcagg cccatagagc acgtagtgga atccgctgga caaagctttt
     2701 gcgtccgatg gcggcgtcag tgttgccgcc tccacattgt tggtcattaa agccgccaaa
     2761 gcttcgggat cgcccccgct ttgcatgaag ctgaaatccc gattcggttc caggccatga
     2821 cgccgcagcg cctggatcgt aaagtagtga gtattgccgc cgatgcggct gaccgcgatc
     2881 cttttccctt tgagatcctc cggtttttta atcgagcctc cagccaccag gctctgtgtc
     2941 ataacgttct tgaccccgcc gacgaaaacc acatccgtgg aaccgcgaac gtaagcgatg
     3001 acgttgccga taccgccggt cagggtcatc tcagcatcgc cgccaagcaa ggccgccgtg
     3061 acgatgctgg aggaggaaat aaataccagg ttgaaatcga gattatattt cttaaatagc
     3121 ccggcctcct gcgccatcca cggcagagac tgcgacatca cccgggcgga gtggatgccc
     3181 accagcttgt caggggccga agcggcatga atcaatgccg gcgcgagaag cagaaaataa
     3241 atcgccgcaa gaatttttcg cgtcatagct ccttcttcct tgcccagagt tgatccatga
     3301 atccgttttt gtccatttcg tctaagtagc gactgtcgat gagttcttgc ggtttgactt
     3361 tctttgcccg cggctcggtt tgggccactt cgtcgagaat cgcctgcaac gcctcggtct
     3421 tgatcgcaag tcgcggctcg agcgccttga tttcggcgtt gtaagcagaa tcaagtacgc
     3481 ttctatccgt aatccgcagg tattttctcc tgctaagaaa cgtgggtaac agtttccgat
     3541 tcgtgaatct cgctgctcaa atcagcctct tctatcgctc caaggaaccc atgtcaaaaa
     3601 ctacttgact ctgccaagat ggttcggctc gaagtcatga ccaaaaacgc tctttccaaa
     3661 gcgggatcgc tttgggattt acgacggtgt caggcggctt gccctcgaga atcgaaagaa
     3721 tgctttgcgc ggcgagccgc tgaccggctt ccagcgagcc cggattattg ccgatgatgt
     3781 gcggcgtcat ggtcagcttg gtcggatcga cctggcgcag gggattgtcc atcggcgtcg
     3841 gttcttcgtc gaaaacatcc aacgccgctc ccgctatgat cccttcattg agagcttgcg
     3901 ccagatcggc ctcgttgaac acttgaccac gcgacgtgtt gacgatatag gcggtcttct
     3961 tcatcatctt gagctgcggt agttggatca tcttgcgcgt ctcccgggtc aaaacgacat
     4021 gaatcgtcac cagatccgat tctctgagca actcctccaa tgagaccatc ttgatcccga
     4081 gaggcgccac cgtttcctga tcgacatacg gatcataacc gagaaacttc agatcccagc
     4141 cgctcagccg tttaacgacg ttacggccaa tgcggccgac gccgatgatc ccgatggtcc
     4201 ggcccatcat cagcatgccg cggttctgcg gctctttcca accgccctgg cgcagcaaag
     4261 cttcattgaa cttgaggcgc ttaaacagca tcaccatcaa tcc
//
LOCUS       DASUTG010000724         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1002368, whole genome shotgun sequence.
ACCESSION   DASUTG010000724 DASUTG010000000
VERSION     DASUTG010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002368"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VF089_16745"
     CDS             <1..453
                     /locus_tag="VF089_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6175660.1"
                     /translation="KQLEIVNRYGRDGSDVGWHFLTGEKSAIEKVTQSAGFTYAWDEA
                     SKQYVHASGIFILTPQGEIARVLYGIDYTPRDIRLALVEAADGTIGNAIDQLLLYCYH
                     YNPLTGKYGFAIMAALRLAGVSTVLAMATYIVTMLRRERRGAHPGAEA"
     gene            457..1410
                     /gene="coxB"
                     /locus_tag="VF089_16750"
     CDS             457..1410
                     /gene="coxB"
                     /locus_tag="VF089_16750"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6175661.1"
                     /translation="MFEDLPLFPPPASTFAGQVDAIYFFLVAMATFFVLLIFGTIIYF
                     TAKYRRRSATERPPPIHIDMRLEILWSVVPLILVMISFGWGASLFVQMSQAPEDALEL
                     RAVGRQWMWKLQHPSGAREINQMHIPVGQPIKVILASEDVIHSFYVPAFRVKMDVVPG
                     RYTATWFEANREGEFHLFCTEYCGTQHSGMIGRVVAMKPVDYQRWLTSQAGEEPMAVS
                     GQRLFQQLGCETCHQTTSTAQGPALAGLFGRQVKLESGESALADEAYIRESILNPHNK
                     IVAGYRPIMPTYQGQISEDALHQIIVYLRSLDRAPEEKSPS"
     gene            1407..3047
                     /gene="ctaD"
                     /locus_tag="VF089_16755"
     CDS             1407..3047
                     /gene="ctaD"
                     /locus_tag="VF089_16755"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6175662.1"
                     /translation="MSVIAERTVAAQARPPRRHYLNVNYGVRSWLFTVDHKRIALLYL
                     VSITLFFFLGGAAAVMFRVELLTPQSDLFEAETYNKLFTLHGLVMIFFFLIPSIPAVL
                     GNFLVPLMIGARDLAFPKLNLLSWYVFIIGGIFTLIAAIMGGVDTGWTFYTPYSSTYS
                     NSYVALTVTGAFITGFSSILTGLNFIVTIHKMRAPGMTWLRLPLFIWSHYATSLIMVL
                     GTPVLAISLVLVALERLFQLGIFDPRLGGDPVLFQHLFWFYSHPAVYIMILPAMGVIS
                     EIVPCFSRKRIFGYTFIAFSSIAIAVLGFFVWGHHLFVSSQSVYAGLVFSFLSMTVAI
                     PSAIKVFNWTATLYKGSIVYRTPLFYALGFIGLFTIGGLTGIFVATLPIDVHVHDTYF
                     VVAHFHYIMVGGAIMGYLGGLHFWWPKITGRLYPEGWARFSALVIFVGFNLTFFPQFI
                     LGYLGMPRRYAAYPDEFQVLNVMSSAGASILGIGYLIPLIYFFWSMRYGPYAGANPWD
                     ARGLEWTTASPPPTDNFELKPRVSQPAYAYEEEEQKVG"
     gene            3040..3687
                     /locus_tag="VF089_16760"
     CDS             3040..3687
                     /locus_tag="VF089_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX6175663.1"
                     /translation="MADSHGAALAHQFDDPEQQVEASTLGMWTFLATEILFFGGMFVT
                     YLVYRLSYPQAFAAASHHLDVVLGTVNTGVLIGSSLTMVLAVYSAQMGRRRGQILFLA
                     ATLLLGLIFLGIKGVEYQHKFHEHLVPGAGFQFNDADPRHAAIFFSLYFAMTGMHAFH
                     MVIGAGLLIWMALRAWRGEFSAGYYTPVEMVGLYWHFVDVVWIFLFPLLYLIGRH"
BASE COUNT          715 a         1075 c         1044 g          988 t
ORIGIN      
        1 aagcaacttg agattgtcaa tcggtatggc cgcgatggca gcgatgttgg ctggcacttt
       61 ctcactggag aaaagagcgc catcgaaaaa gtgacccaaa gcgccggatt cacctacgcc
      121 tgggacgagg ccagcaagca gtacgtacac gcatccggaa tttttattct tacaccgcag
      181 ggagaaatag cccgggttct ttacggcatc gattatacgc cgcgcgacat tcgcctggcc
      241 ttggtggaag ccgccgatgg caccatcggc aacgccatcg atcaactgct gctctactgc
      301 tatcactata acccgctcac cggaaaatat ggattcgcga ttatggcagc tctgcgcctg
      361 gccggagtgt caacagtgct ggccatggcg acgtatatcg tcacgatgct gcggcgcgag
      421 cggcgcggcg cgcatccggg agcggaggct tagcgcatgt ttgaagacct tccgctgttt
      481 ccgccgccag cctcgacctt cgccgggcaa gtcgatgcca tctatttctt tttagttgcg
      541 atggccactt ttttcgtcct tctgatattc ggcacgataa tttacttcac ggctaagtat
      601 cggcggcgct cggcaaccga gcggccgccg ccgatccaca tcgacatgcg cctggagatt
      661 ctctggtcgg ttgtcccttt gattttggtt atgatcagtt tcggctgggg cgcaagtctg
      721 ttcgtgcaaa tgtcccaagc gccggaagac gccttggaac tgcgcgcggt gggacggcaa
      781 tggatgtgga agttgcaaca tccgagcggt gcccgggaaa tcaatcaaat gcatattccg
      841 gtgggtcagc cgatcaaggt tatcctcgcc tcggaggatg tcattcatag tttttatgtg
      901 ccggcgtttc gggtcaagat ggatgtcgtc cccggcagat atactgcgac ttggttcgaa
      961 gcgaaccggg aaggcgagtt tcacctgttc tgcaccgaat actgcggcac gcaacattcc
     1021 ggaatgatcg gcagagtggt cgctatgaaa cctgtggact accaaaggtg gcttacatcc
     1081 caggccggtg aggaaccgat ggcggtcagc ggccagcgac tatttcagca actcggctgt
     1141 gaaacctgtc accagaccac ctccacagct cagggccccg cgttggccgg cctgttcggc
     1201 cgtcaagtca aactcgagtc cggcgagtcc gcgctggccg atgaggcata catccgcgaa
     1261 tcgatcctca atccgcacaa caagatcgtc gccggatatc ggccgattat gcccacctat
     1321 caaggtcaga tcagcgaaga tgcgctgcac caaatcattg tttatctaag gtcgctcgac
     1381 agagcgccgg aggaaaagag cccctcatga gtgtgatagc cgaaagaacc gtcgccgcac
     1441 aggcgcgtcc gccgcgacgg cattatctga acgtcaacta cggcgttcgt tcttggctat
     1501 ttaccgtcga tcacaaacgt atcgcattgc tctatttggt ttcgatcact ttattttttt
     1561 tcctgggcgg cgccgcggcg gtgatgtttc gcgtcgagtt gctgacaccc caatccgatc
     1621 ttttcgaagc cgagacttac aacaaacttt tcaccctgca cggattggtg atgatcttct
     1681 tttttctgat cccatcgata cccgcggtgt tggggaactt cctggtgccg ttgatgatcg
     1741 gcgcccgcga tttggcgttt cccaagttga atctcttgag ttggtacgtg ttcatcatcg
     1801 gcgggatctt cacgctcatc gccgcgatca tgggaggggt cgataccggc tggactttct
     1861 acaccccgta cagcagcacc tactccaaca gctatgtcgc ccttactgta accggagctt
     1921 ttatcaccgg attctcgtcc atcctgaccg gcctcaactt catcgtgacg attcacaaaa
     1981 tgcgcgcgcc gggaatgacc tggctgcgct tgccgctatt tatttggtcg cattacgcca
     2041 ccagcctgat catggtactc ggcactccgg ttttggccat cagcctggtt ctggttgccc
     2101 tcgagcggct gtttcaatta ggcatcttcg atccccgctt aggcggcgat ccggtgctgt
     2161 tccagcatct attttggttt tattcccatc cggcggtcta tatcatgatc ctgccggcca
     2221 tgggcgtgat cagcgagatc gttccgtgct tctcgcgcaa acggattttc ggctatacct
     2281 ttatcgcttt ctccagcatt gccattgcag tccttggctt ctttgtctgg ggtcatcacc
     2341 tgttcgtcag cagccaatca gtttatgccg ggctggtctt ttcctttctc agtatgacgg
     2401 tggcgattcc gtcggcgatc aaggtattca attggacggc gacgctgtac aaaggctcga
     2461 tcgtttatcg tacgccgttg ttttacgctc tcggattcat cggtttgttc actatcggcg
     2521 ggttgaccgg aatattcgta gccactctgc cgatcgacgt gcatgtccat gacacctact
     2581 ttgtggtcgc gcattttcat tacatcatgg tcggcggcgc cattatgggg tatctgggcg
     2641 gcctccattt ctggtggccg aagatcaccg gtaggcttta tccggaagga tgggcgcgtt
     2701 tctcggcgct ggttatcttt gtcggcttta acctgacgtt ttttccgcaa ttcattctgg
     2761 gttatctcgg catgccgcgg cgctatgcgg cttatcccga cgagtttcag gtgcttaacg
     2821 tcatgtcatc ggcgggcgct tcgatcctcg ggataggcta cctgattccg ttgatttatt
     2881 tcttctggtc gatgcgctat ggcccgtacg ccggcgccaa tccttgggac gcgcgaggct
     2941 tggaatggac cacagcttcg ccgccgccga cggataactt cgagcttaag ccccgagtat
     3001 cgcaaccggc ctacgcctac gaggaagagg agcagaaagt tggctgattc acatggcgcc
     3061 gccctggcgc atcagttcga cgatcccgag cagcaggtcg aagcgtcaac tctcggcatg
     3121 tggacgtttc tggctacgga gattcttttc tttggcggga tgtttgtcac ttatctcgtt
     3181 tatcgcttgt cttatccgca ggcgtttgcc gccgccagcc accatcttga tgtcgtgctc
     3241 ggcacggtca acacaggagt tttgatcggc agcagcctga cgatggtcct ggcggtttac
     3301 agcgcgcaga tgggccggcg gcgcggccag atcctatttc ttgcggcaac gttgctgctg
     3361 ggcctgattt tcctcggcat caagggtgtt gagtaccagc acaaatttca tgagcacttg
     3421 gttcctggcg ccggctttca atttaacgac gccgatccgc gtcatgcggc gattttcttc
     3481 tcgctctact tcgcgatgac cgggatgcac gcttttcaca tggtgatcgg cgcggggctg
     3541 cttatctgga tggcgctgcg cgcatggcgc ggagaatttt cagcgggcta ttatactccg
     3601 gtggaaatgg tcggcctcta ctggcatttc gttgatgtgg tatggatttt tttgttcccg
     3661 ctgctttacc tgatcggacg gcactgatgg attcccacgc cgcagcagca caaagaaaaa
     3721 cttattttct ggtattcggc gccctgctgc ttcttacctt agtcaccacg ggagtagcat
     3781 tcatcaatct cggcccgctt aacacaccgg ttgcactggc ca
//
LOCUS       DASUTG010000725         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_555924, whole genome shotgun sequence.
ACCESSION   DASUTG010000725 DASUTG010000000
VERSION     DASUTG010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555924"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /gene="rplC"
                     /locus_tag="VF089_16765"
     CDS             complement(<1..149)
                     /gene="rplC"
                     /locus_tag="VF089_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284343.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6175664.1"
                     /translation="MTGLIGKKLGMTQFYSSDGNVVPVTVIETGPCVVVQKKETAIDG
                     YNALQV"
     gene            complement(191..499)
                     /gene="rpsJ"
                     /locus_tag="VF089_16770"
     CDS             complement(191..499)
                     /gene="rpsJ"
                     /locus_tag="VF089_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6175665.1"
                     /translation="MNEKIRIRLKAYDHRVLDQSVGEIVETVKRTGGRVAGPIPLPTR
                     IERFTVNRSPHVDKKSRDQFEIRTHKRLLDILEPTQQTIDALGKLDIAAGVDVEIKLQ
                     "
     gene            complement(496..2592)
                     /gene="fusA"
                     /locus_tag="VF089_16775"
     CDS             complement(496..2592)
                     /gene="fusA"
                     /locus_tag="VF089_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6175666.1"
                     /translation="MPRTVPLDRTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVH
                     EGTATMDWMVQEQERGITITSAATTCLWRDHRVNIIDTPGHVDFTIEVERSLRVLDGA
                     VGVFCSVGGVEPQTETVWRQADKYQVPRIAFVNKMDRLGADFFRVVQMIRDRLGARAV
                     PLQLPIGAEDKFIGVIDLVRMKAIVWEDESLGAKFRDEAIPEEMASAAAEYREKLIEA
                     AADSDETIMEKYLEGQEISEAELTKAVRSATLSIKIIPVLCGSAFRNKGVQPLLDAVI
                     EYLPSPLDIPPVKGIDPDSQQSDERPAKDDAPFSALAFKIMTDPFVGTLSFFRVYSGS
                     LTAGSSVYNSTKGKRERIGRLLKMHANKREEIKEVYAGDIAAGVGLRTATTGDTLCDE
                     NDPIILESIEFPEPVISIAIEPKSKADQEKLGLSLQKLAAEDPSFRVRTDEETGQTII
                     SGMGELHLEIIVDRLLREFNVGANVGKPQVAYKETVRKAVEQQGKFIRQTGGRGQYGD
                     VWIKLEPQEPGAGFEFVDAVKGGAIPREYIPAVEKGIKEATENGALAGYPIVDVKVTL
                     FDGSYHDVDSSEIAFKIAGSMAFKEAARKASPVLLEPIMSVEVVVPEDFMGDVIGDLN
                     SRRGKVLGMDSRPAAQAIDARVPLAQMFGYATDLRSMTQGRATYTMQFSHYEPVPANV
                     AEGIIAKLDGKSDDKK"
     gene            complement(2659..3129)
                     /gene="rpsG"
                     /locus_tag="VF089_16780"
     CDS             complement(2659..3129)
                     /gene="rpsG"
                     /locus_tag="VF089_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6175667.1"
                     /translation="MPRKGEVRKREVLPDPKFGEKLVTKFVNTIMNQGKKSVAESILY
                     RSLDIVAAKTKEDSIGIFKKAIENTRPSVEVRSRRVGGATYQVPVEVRPQRRISLSMR
                     WLIQAARDRSEKSMEEKLAAELLDAANNRGTAIKKKEDTHRMAEANRAFAHYRW"
     gene            complement(3131..3502)
                     /gene="rpsL"
                     /locus_tag="VF089_16785"
     CDS             complement(3131..3502)
                     /gene="rpsL"
                     /locus_tag="VF089_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6175668.1"
                     /translation="MPTISQLVRESREKQQRKNTAPALQGCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTL
                     DSIGVQERRQGRSKYGAKKPK"
     gene            complement(3598..>4005)
                     /locus_tag="VF089_16790"
     CDS             complement(3598..>4005)
                     /locus_tag="VF089_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6175669.1"
                     /translation="VIVRQMLRRVRIKEVGDTEFLVGDQVEKWRFDEENQRAIAGGGK
                     PAVAEPLLLGITKASLSTESFISAASFQETTKVLTEAAIHGKIDLLCGLKENVIMGRL
                     IPAGTGVARYSKAEIEIDAPEGFEEDMSQAAGA"
BASE COUNT          888 a         1189 c          925 g         1003 t
ORIGIN      
        1 acctgcaggg cattgtaacc gtctatcgct gtttccttct tctgcaccac aacacagggt
       61 ccggtctcaa tgaccgtaac gggaaccaca ttcccgtccg aggagtagaa ttgcgtcatc
      121 ccaagttttt ttccgataag gccagtcata tgattatctc aggcgaagct tctctagcag
      181 agttgtctcc ctattgaagc ttgatctcaa catccacacc cgcagcaata tcgagttttc
      241 ccaatgcgtc aatggtctgc tgagtcggtt ccaaaatatc aagcaatctc ttatgggttc
      301 gaatctcaaa ttggtcacgg gattttttgt cgacgtgtgg cgaccgatta accgtaaacc
      361 gttcgatccg tgtcggcaaa ggaatcggtc cagccactct gccaccggtg cgttttaccg
      421 tctcgacgat ctctccaacc gattgatcca acacccgatg atcgtaggcc tttagccgaa
      481 tcctgatttt ctcgttcatt ttttgtcatc cgacttgccg tctaacttgg caataatccc
      541 ctcggcaacg ttggccggca ccggctcata atgtgagaac tgcattgtgt aggttgctcg
      601 cccttgggtc atcgaacgca ggtcggtagc ataaccgaac atttgtgcca gcggcacccg
      661 cgcatctata gcctgagcgg ccggccgcga atccatcccc aaaaccttac cgcggcgcga
      721 gttgagatcg ccaatcacgt cgcccatgaa atcttcaggg actaccacct ccaccgacat
      781 aataggctcg agcaggaccg gacttgcctt gcgggcggcc tctttgaacg ccatcgagcc
      841 ggcaatcttg aaagcgattt ccgatgagtc gacatcatga tagctgccgt cgaataacgt
      901 gaccttcaca tcaacgatcg gatagcccgc taacgcgcca ttttccgtcg cctctttaat
      961 gcctttttct acggctggaa tatactcgcg cggaatcgcg cctcctttga cagcgtcgac
     1021 aaattcgaat ccggctccag gttcttgcgg ctccagtttg atccagacgt caccatattg
     1081 acctcgacca ccggtttggc gaatgaactt gccctgctgc tcgaccgcct tgcgaacggt
     1141 ttccttatag gcaacctgcg gcttacccac gttggccccg acgttgaatt ctcttaatag
     1201 tcgatcaacg atgatctcga gatgaagctc acccatcccg gaaataatcg tctgtccggt
     1261 ctcttcgtcg gttcgcactc ggaacgacgg atcttcggcg gctagcttct gcaatgacaa
     1321 tccaagcttt tcctgatcag ccttgctttt cggttcgatt gcaatcgaaa ttaccggctc
     1381 agggaattcg atcgattcga gaatgatcgg atcgttctca tcgcacaaag tgtcgcccgt
     1441 agttgcagtt cgcagaccca ctccagcggc aatatcaccg gcataaactt cctttatctc
     1501 ttcacgcttg ttcgcgtgca tcttcaaaag acgaccgatt ctttcgcgct tgcctttggt
     1561 tgaattgtaa acactggagc ccgcggtcaa cgacccggag tagaccctga aaaagctcaa
     1621 cgttccaacg aaggggtcgg tcataatttt gaaagcaaga gccgaaaacg gagcatcgtc
     1681 cttggcgggg cgttcgtcgg attgctggga atcaggatcg atgcctttaa ccggcgggat
     1741 gtccaacgga gagggcaagt actcaatcac cgcgtcgagc aatggctgaa cacctttgtt
     1801 gcggaacgcc gaaccacaca ggaccggaat gatcttgatg ctcaaagtag cactcctgac
     1861 ggccttcgtc agctcagctt cgctgatttc ctggccctcc aagtacttct ccatgattgt
     1921 ctcgtccgaa tccgcagccg cttcgatcaa tttttcacga tactccgccg cagccgacgc
     1981 catctcttcc ggtatggctt catcgcgaaa cttcgccccg agactctcat cctcccaaac
     2041 aatcgccttc atgcgcacca aatcaatgac gccgatgaat ttatcctcgg caccaatcgg
     2101 tagctgcaac ggaaccgcac gcgcgccgag acgatcccta atcatctgga cgaccctgaa
     2161 gaaatccgct ccgagtcgat ccatcttatt aacgaaggca atgcgcggaa cttggtactt
     2221 atcggcctgt cgccaaaccg tctcggtctg cggctcgacg ccacccaccg agcagaaaac
     2281 ccctaccgca ccgtccagaa cccgtagcga acgttcgacc tcgattgtaa agtcaacgtg
     2341 ccccggcgta tcgatgatat tgacccgatg gtcccgccat aaacacgtgg ttgcggctga
     2401 ggtgatagta attccacgct cctgctcctg gaccatccaa tccatggtcg cggttccctc
     2461 atggacctca ccgatcttgt aattgatccc cgtgtagtaa aggatccttt cggtggtcgt
     2521 tgttttgccc gcatcaatgt gcgccatgat gccgatattg cgggttctat ctaagggaac
     2581 agttctaggc attgctctac accgggaaaa aaaggaggcg gatcaaccgc ctccttttta
     2641 cgaatatttt ttctcaggct accatcgata atgagcaaag gcgcgattcg cctccgccat
     2701 acgatgagtg tcttcttttt tctttattgc cgtgcctcga ttattagcgg cgtccaataa
     2761 ttcagccgcc aacttttctt ccatcgactt ttcgctgcga tcgcgcgccg cctgaatcag
     2821 ccagcgcatg ctcaaagaaa tacgccgttg cggtctgacc tccacaggaa cttgatacgt
     2881 ggcgccgccc acgcggcgcg atcgaacttc gaccgacggc cgggtgttct caatcgcttt
     2941 cttaaaaata ccgatcgagt cttccttggt cttggccgca acgatgtcta aggatcggta
     3001 gagaatcgat tcggcgacgc tcttcttccc ctgattcatg atagtgttaa cgaacttggt
     3061 aacaagcttc tcgccgaatt tcggatccgg caaaacctct cgttttctta cttccccttt
     3121 acgtggcatg ttacttcggt ttcttcgctc cgtatttgga acgaccttgc cgcctctctt
     3181 gtacaccaat cgaatcgagc gttccacgaa tgatgtgata gcgaaccccg ggaaggtctt
     3241 tgacgcggcc tccacgaatc aagacaacag aatgttcttg caggttgtga ccaacccccg
     3301 gaatgtacga agtcacctca atcccgttag tcagccgaac gcgagccact ttacgtaacg
     3361 ccgaatttgg cttcttcggc gtcgaagtgt aaacgcgcgt acacaccccc cgcttttgcg
     3421 ggcaaccctg caaagccggc gcggtgttct tacgctgttg cttctctctg ctctcgcgaa
     3481 ctaattgact aattgtaggc atacgtctat tcgatcaccc aaaatgaatt gattgataaa
     3541 gcgacaactt ccggggaacc actttagtta acaaattaat cccatcatgt caaaagccta
     3601 cgctcctgcg gcctgtgaca tgtcctcttc gaatccttct ggagcatcga tttcgatttc
     3661 ggctttcgag tatcgggcga ccccggtccc tgctgggatc aatcggccca tgatgacgtt
     3721 ctccttaaga ccgcaaagta aatcaatctt gccgtgaatc gcagcttcag tgagcacttt
     3781 cgtcgtttcc tgaaacgaag cggccgagat aaagctctcg gtcgatagcg aggccttggt
     3841 gatgccgaga agcagcggct cagccacagc cggcttgccg cctcctgcga tcgcacgttg
     3901 attctcctca tcaaagcgcc atttctccac ttgatcgccg acgaggaatt cggtatcccc
     3961 tacctctttg atgcgcaccc ggcgtaacat ctgtctaacg atgac
//
LOCUS       DASUTG010000726         4463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1148218, whole genome shotgun sequence.
ACCESSION   DASUTG010000726 DASUTG010000000
VERSION     DASUTG010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148218"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(816..1193)
                     /locus_tag="VF089_16795"
     CDS             complement(816..1193)
                     /locus_tag="VF089_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175670.1"
                     /translation="MKILIVEDNPDMRDLIALRVQLTGYIPILASHGKEGVEKATAEK
                     PDLILMDMMMPVMDGWEATRTLRSSRETKEIPILALTALFRSEDLKSCLDCGCSGYIV
                     KPFTGVDLQRKIRELLDPKELPA"
     gene            complement(1998..2210)
                     /locus_tag="VF089_16800"
     CDS             complement(1998..2210)
                     /locus_tag="VF089_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175671.1"
                     /translation="MSILTAVSNMAFSVVWVAEQLKYFEQEGIRAKITPAGGGAPCQN
                     AVVGRWAHLCASSPEGLLLAQSKARV"
     gene            complement(2373..2852)
                     /locus_tag="VF089_16805"
     CDS             complement(2373..2852)
                     /locus_tag="VF089_16805"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175672.1"
                     /translation="MNNVIDALAQSPPQAESTEASGKGYVLIPLGLGEVTELTNYPYL
                     VLMSRPDWAEQNPKLVSATARAVSRAGALFHTNPDQAKAALRAHRFFDPKRLDDDLFN
                     LAYSMVVNAIPAWGNMDQEGWQKELNCAAGAGIIKEPATAASAKEGVFWTNKYVGKP"
     gene            complement(2791..3498)
                     /locus_tag="VF089_16810"
     CDS             complement(2791..3498)
                     /locus_tag="VF089_16810"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175673.1"
                     /translation="MGRMYGAVIRAPVFVLRKRGENDHRIRTAACTLKPAFIAVIAIF
                     LAIPGKTTAQQSELADVTVATAVGNLAYSAIWVAEQLEYFEQEGVRAKITVAGGGAPC
                     QSAVVGRSTHFCASSSEGLVLAKQEGASLIAVQAHNRNVTLGIAVRKAIADKHKLTRK
                     SPLNERLKVLTQLGTIGATSPGAVSEQIFKFLVRKIGSEQTEVRIPGRQRVGSRLNEQ
                     RHRRLGAVPAASGKHRG"
     gene            complement(3533..>4463)
                     /locus_tag="VF089_16815"
     CDS             complement(3533..>4463)
                     /locus_tag="VF089_16815"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175674.1"
                     /translation="PNFAFAAIWVAEQLKYFEEEGVRVKITPASGGSPCQSAVVGRSA
                     HFCASSSEGLVLAQVEGAPLIAIQAHNRALTLSVVLRKAIVDKLGLTRESPIDARLKA
                     LLQVGTIGATSPGAVSEQIFKFLVKKAGGDPSKLKFAYLGAAELPASLMNNVVDTYAL
                     SPPSAEVTEPSGKGYVLIPLGKGEVPELTDYPYEVLMVRPDYLESNAKTATAVARAIS
                     RGGALFRSKPEAAKSALRSHRLFNKQRIPDDVFDLSFDMVVNAMPAWGTMNQDGWQKV
                     VNFASGAGIIKDPSKTPSAKEGVLWTNKYVGKP"
BASE COUNT          994 a         1232 c         1174 g         1063 t
ORIGIN      
        1 cgcagcagtt ggttcaacct gcgaagtcgt tggtgaggac tccgacatta acttagcgtc
       61 agggtgtttt tcgcggtacc agcggctatc acgtcggaag aaagaccact gccctttcat
      121 cctttcatcc gtagatcgtt gggcaacagc gacgatacta gacgggaacg acgcttggcg
      181 agatctgatc aactctctga tcacacgtcg aaaattcaac accgccagcg gaggttgcgt
      241 agctatatat cgcgcgggcg ggctacgagt cagcggcgct ttgcgattta agattatcaa
      301 atgagaacgg acgcggcaca ccacaacgag ggcacgcatc tgcagacctg ttaattcgcg
      361 ctgcgcatcg aagacaaagc gttttacgcc gtcgaatcag gagaagtaag gcaataatga
      421 tagccgctac tggaacgtat tcattcatac catgtccagt gatccgtatg acaacttgtt
      481 gacgctcacc tggcaccaca agcgtaaatg catttcaccc cgagtttacc tacagcacgg
      541 caatgattaa gtatgcagca aataaataaa gcgccaatgt accaatcatt attaatgaga
      601 tcagtgatat gtcttcgaca tgctttgacc agaatttacg gggcttgatc atataattat
      661 ctcgcaacgg cgcatgatct tcgaccaaaa tgcggcggtt gaatatcacg gatgttttct
      721 ttttcaagac atcttaagcg gccgcgacca gttcgcgggc gatattcaat aatgccgaga
      781 agcggccatg tagtttgcga cagccgacgc tgtttttatg ccggtaattc cttcgggtct
      841 aacaattctc ggattttgcg ctggagatcc acacccgtaa acggtttaac gatgtagccg
      901 ctgcagccac agtccaaaca cgacttaaga tcctcagaac gaaacagcgc cgttaatgcc
      961 aggattggga tctccttggt ttcgcgagag gagcgaagtg ttcttgtcgc ttcccacccg
     1021 tccatgactg gcatcatcat atccatcagg atcaaatccg gcttctctgc ggtagccttc
     1081 tctacgccct ccttaccgtg acttgcgagg atcgggatat agccggttag ctgtactctg
     1141 agagctatca agtcgcgcat atccgggtta tcttctacga tcaagatttt cattcgtcca
     1201 ccttatccga accctcataa gcgtactgaa cattgacagt ggcgccggga acgaagaaga
     1261 tcggggagtg tcgtaacaag ttgcagttct tcgccccggc tataggtgag tgagcaagca
     1321 ttggaccctc actattgatt ggacagccga tcactctgca gatatttgga ccaggttcgg
     1381 gagttctgtc ccggcacgct cgacggcaac tttgcctgtt accccagagc catcgtacgc
     1441 atcgggttta cttgtcaatg ggtcggtcaa gagaccggaa cgcagttgga caaacattaa
     1501 caatcgcacc gggcatgcac tgtgctttga tgaacctacc tcaggttaga tgtgctcagt
     1561 cttcaaagat ctgcgcagtg ttcgtaggag taatcgagca tgtcggagaa tcatcaagga
     1621 gccgtttgcg ccacgaaggc atcactttac gttggatcag atcagggtac ggcgggttag
     1681 ctctttctat cagcgcttac tgaggtcagc tctgggggct gatacgttag ctgaggcatc
     1741 gctcgaagat ttttgtatgg tggtcctgtt actcttttta ctatttacca ctctgcttct
     1801 gacgtgggtg cttttgtaag tgttggtgcc tcgctcaaca tcgatcacat tgttcattag
     1861 cgccgatggt gccaagctcg gttaggactt tgatgagatc cttgagtggg cttttgcgcg
     1921 tgaatttgaa ctgtcggtga tcgccttgcg cacggcaata ctcaaagcca aattccggtt
     1981 gtccgcctga attgcgatca aacgcgcgcc ttcgattgcg ccagcagcag tccttcgggg
     2041 ctcgaagcgc aaaggtgcgc ccagcggccg actaccgcgt tctgacatgg cgcgccgccg
     2101 cccgcgggcg tgatcttggc gcgaatgcct tcctgttcaa aatacttcaa ttgctcggcg
     2161 acccacacga cggagaaagc catgttcgac acggcggtaa gaatgctcac gtccccaagc
     2221 tctttttgtt gcgcatggat tcgccgagga caacatcaaa ccaaggagca tggtgaggcc
     2281 acagagcaaa tagagagtga ctttgcgcaa agggaataag ccatcctcac tgtgtctctc
     2341 cgcaagaact taatgatcga acaagcaagt gtctaaggtt tgccgacata cttgttggtc
     2401 cagaacacgc cttctttagc cgatgcagcc gtggccggtt ctttaattat cccggcaccg
     2461 gcggcacagt tcagctcttt ttgccaccct tcttgatcca tgtttcccca ggccggaatc
     2521 gcgttgacga ccatggaata ggcgagattg aataaatcgt cgtcgagccg cttcggatcg
     2581 aaaaatcggt gtgcccgcaa cgcggccttg gcttgatcgg gattggtgtg aaagagagcg
     2641 ccggcacgtg agactgcgcg cgcggtggcg ctcactagtt ttggattctg ttctgcccaa
     2701 tcggggcgcg acatcagaac cagatatgga taattcgtca gctctgttac ttcgccgagt
     2761 cctaaaggga tcagcacgta accttttccg ctagcctcgg tgctttccgc ttgcggcggg
     2821 gactgcgcca aggcgtcgat gacgttgttc attaaggcgg gaaccaactc gttgccgccc
     2881 aggtatgcga acttcagttt gctcggatcc gatctttctt accagaaact taaatatctg
     2941 ctccgatacc gcgcccggac tggtagcgcc aatggtgccc aactgagtca atactttcag
     3001 acgctcgttc agtggactct ttcgggtaag tttatgtttg tcggcaatgg ctttacgtac
     3061 cgcaatgccc agcgtcacgt tacgattatg ggcctgtacc gcaatgagcg atgcaccttc
     3121 ctgtttagcg aggaccagcc cctcggagct cgaggcgcaa aaatgtgtcg atctacccac
     3181 gaccgccgac tggcatggcg ccccaccgcc ggcaacggtg atcttcgccc gcacaccttc
     3241 ctgctcaaag tactcaagct gttcggcaac ccaaatcgcc gagtacgcca agttccccac
     3301 tgcggtcgcc actgttacat cggcaagttc gctttgttgt gcggttgttt ttccagggat
     3361 cgccaagaaa atggcaatga cagcaatgaa ggctggtttg agggtacacg cagcggtgcg
     3421 aatacggtga tcattttccc ctctcttacg aagaacgaag actggcgctc taattactgc
     3481 gccgtacatc ctgcccattt ctcctcctat tgctcgatga aatgcaattg ggctatggct
     3541 tgccgacgta tttgttggtc cagagcacgc cttctttagc cgacggtgtt ttggatggat
     3601 ctttgatgat ccccgcgccg ctggcgaaat tgaccacctt ctgccaacca tcctggttca
     3661 tcgtgcccca agccggcatt gcgttgacga ccatgtcgaa agaaagatca aatacgtcgt
     3721 ccggtatgcg ctgcttattg aaaagacggt gactgcgtaa ggcgctcttt gcggcttccg
     3781 gtttgctgcg gaagagcgcg ccgccgcgcg agatcgcgcg tgcgaccgcc gtggcggtct
     3841 ttgcgttcga ctcaagataa tcgggccgaa ccatcaggac ctcgtaagga taatccgtca
     3901 gctccggcac ctcgcccttc ccgagcggaa tcaagacata gcccttgccg ctcggttcgg
     3961 tcacctcggc ggagggcggc gacagggcgt aagtgtcgac cacgttattc atcagcgatg
     4021 ccggcagttc cgcagcgccg agataggcaa acttgagttt ggacggatcg cccccggcct
     4081 tctttaccag gaacttgaaa atctgctcag aaaccgcgcc cggactagtg gcgccgatag
     4141 tgcccacctg gagcaacgcc ttaagccgcg catcgatggg gctctcccgg gtcaacccca
     4201 gtttatccac gattgctttt cgcaggacca cgctcaacgt tagcgctcgg ttgtgcgcct
     4261 ggatggcaat gagcggcgct ccctcgactt gcgccaatac cagaccttcg gagctggaag
     4321 cgcaaaaatg cgccgaacgc ccgaccactg ccgactggca gggcgagcct cccgaagcag
     4381 gcgtaatttt cacccgcacg ccctcctcct cgaaatattt cagctgctcc gcaacccaga
     4441 tggcggcgaa tgcgaaattg ggc
//
LOCUS       DASUTG010000727         7450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2188745, whole genome shotgun sequence.
ACCESSION   DASUTG010000727 DASUTG010000000
VERSION     DASUTG010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188745"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /gene="gcvPB"
                     /locus_tag="VF089_16820"
     CDS             complement(<1..951)
                     /gene="gcvPB"
                     /locus_tag="VF089_16820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6175675.1"
                     /translation="MSERSRTDPPQPAQSAPLLFESGSRGRTAFAWPDKTPPATDSIP
                     ESYLRHDIPDFPELGELELLRHFTRLSQRNYAIESQFYPLGSCTMKYNPKVNEVVARY
                     PGFSLVHPLAPPAVNQGALELLFGLEQMLAEIGGMDDVSLQPSAGAQGELTGLMLIRA
                     YLSAQGNPRKKIIVPDTAHGTNPASSTLCGYDVVQISSNEQGVIAAAAVAEAMDEDIA
                     ALMVTNPNTLGIFEKNIEAVADVVHGKGGLVYLDGANLNALMGIAKPGHMGVDVLHMN
                     LHKTFSTPHGGGGPGAGPVGVKAFLRDFLPTPRIVKSANGF"
     gene            complement(929..2275)
                     /gene="gcvPA"
                     /locus_tag="VF089_16825"
     CDS             complement(929..2275)
                     /gene="gcvPA"
                     /locus_tag="VF089_16825"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX6175676.1"
                     /translation="MRYTPHTEADKKRMLDAIGAGSIEDLFRHVPEKFRSTAKIHLPD
                     GLSELTVRQRLAALAAKNAAANDWDLYLGGGIYHHFIPSAVDAVISRSEFSTSYTPYQ
                     PEVSQGTLQVLFEYQTLVCQLTGMEVSNAGVYDGASASAEAILMSRRIQSSSRRRVLV
                     SRALHPQYRAVIATYLRNLSDLSLEEIPFDGSGATDFDHLARQLNEGTMCVLVGYPNF
                     FGVIENLAPLHELCGRAGAQLITATTEPLAFGLLKPPGAFGVDIAVGEGQSLGVPMAL
                     GGPGFGFFASQRKFMRNLPGRLVGETVDSEGRRGYVLTLATREQHIRREKATSNICTS
                     QTLCTMAATVYMSFLGRTGMRRLAEVNVARAHEAYQRLLRESKLEGCFRTPFFNEFVV
                     RGKQLDEIMRRCSERRIIPGISLARWYPELPDCLLVCVTEMNDTEGIDRLVRAISN"
     gene            complement(2285..2722)
                     /gene="gcvH"
                     /locus_tag="VF089_16830"
     CDS             complement(2285..2722)
                     /gene="gcvH"
                     /locus_tag="VF089_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6175677.1"
                     /translation="MEIPEGLRYSKEHEWVLVEGKTATIGITEYAQEELGDIVFVELP
                     EIGEKIVKDDPFGAVESVKAVSDIYAPVSGTVLEINDSLPDSPEIINDDAYGDGWMIR
                     VEMQDMDDLKDLMTAEEYAEYIEQQKEEDDEDDEAEEDDEEEE"
     gene            complement(2753..3832)
                     /gene="gcvT"
                     /locus_tag="VF089_16835"
     CDS             complement(2753..3832)
                     /gene="gcvT"
                     /locus_tag="VF089_16835"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX6175678.1"
                     /translation="MNRTPLYSAHRRAGGRMVDFAGWEMPVQYSGVIAEHLAVRSRAG
                     LFDVSHMGEIAVCGAGAEAMCQRLTANDVARMQVSQAQYNLLLNDRGGVIDDVIFYKR
                     QPDDYLICVNASNSGKDFQWLRQHATGDIQVENVSAAYAQLALQGPLAERIMQPLAAI
                     ALSDIRTFYFAESEVAGTRCLIARTGYTGEAGFELYCSSQDAEKLWRDLLDAGSPAGL
                     VPAGLGARDTLRLEKAYPLYGHELDDSTTPLEAGLEWLVKFSKGSFIGCEVLLRQKQA
                     GVKRKLVGLELIEPGIARSEYRLLKEGRCIGKVTSGTRSPTLGRSIALGYVEAEEARA
                     ENILEVEIRNKAVRAKIVPLPFYRR"
     gene            complement(3871..4731)
                     /gene="folD"
                     /locus_tag="VF089_16840"
     CDS             complement(3871..4731)
                     /gene="folD"
                     /locus_tag="VF089_16840"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX6175679.1"
                     /translation="MASIIDGKAVAKEVQKRIKEEVDGLERRWGMAPGLAVVLVGDDP
                     ASHIYVKNKEKACKEVGIKSSEHLLPATVSEKELLSLVQSLNKDKDVHGILVQLPLPA
                     HIRSERILEAVSPFKDVDGFHPMSQGMLVIGANGFRPCTPMGVMKLLEAAGCALKGKN
                     AVVVGRSNIVGKPVALMLLEKHATVTICHSRTASLRDEVARADVLVVAIGKAGLVRGD
                     WIKPGAVVIDVGVNRLPSGKLTGDVEFETAKERASAITPVPGGVGPMTICMLLYNTLK
                     AAKDSIQRER"
     gene            5105..6199
                     /locus_tag="VF089_16845"
     CDS             5105..6199
                     /locus_tag="VF089_16845"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6175680.1"
                     /translation="MNQPRSLEEIKNDIRARLGHRAPFLHADKAEAEEALGRMSSVQG
                     EIWAQSWNELGARWEAKAREAESAGNKQEAKENYLKSYGYYGIARHPFPSTPGKHHGY
                     LKTREMFLAASKYFEIPIERVSIPFQGKQIIGHLRLPKKVPAPLIMHWGGIDNWKEER
                     HSFAESFVKEGWGCFVMDSPGTGECPVLAGPEAHQVHTTALEYLRERPEVDTTRIAVV
                     GASFGGHWSTKLAHLAPDKLRAAVNWGGGIHYFFQPEWQERSRNADSYLFDLIEARAN
                     LFGKQSFDELCQVMPALSLLDQGWLEKPCAPLLIVNGKEDKQVPLEDFYLLLEHGDPK
                     TIRLFPGGHMGAIPNIFKTVITWLHQRLDG"
     gene            6201..6455
                     /locus_tag="VF089_16850"
     CDS             6201..6455
                     /locus_tag="VF089_16850"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175681.1"
                     /translation="MSLYQLQKLIYDVNRNPERRDAYRADKTGFLARYELTPEEQAAI
                     ADLDVRKLYSYGVHPLLLRPFTLLHRVSNEDYAKALAGLE"
     gene            6459..7274
                     /locus_tag="VF089_16855"
     CDS             6459..7274
                     /locus_tag="VF089_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175682.1"
                     /translation="MPIVFACAASHAPGMTAWTEAAPQEQRDKILGSYHKLAEMLAAS
                     KPDALVVVSVEHWANFFLNQMPTFCIGRAQYYHGPVEEWLKISKTRVPGDAALGAQLI
                     EACLDSGFDPAFSDELLFDHATMLPLHFVNPGMTIPVVPLIINTLTPPMPTPKRCFEL
                     GRVLGQALEQQAKRIALIATGGLSHWPGEAKMGKINVAFDEQFLQLLIDGKRARLAEY
                     SHAQIDKEAGSGGHEIRTWITVAAAVEDWRASLLAYEPVHPWATGCGLVAFTA"
BASE COUNT         1681 a         2100 c         2001 g         1668 t
ORIGIN      
        1 aaaaccgttg gcgctcttta cgatccgcgg cgtcggcaga aaatcgcgca ggaaggcttt
       61 taccccaacc ggaccggcgc ctggtccgcc accgccgtgc ggtgtggaaa aggttttgtg
      121 caaattcata tggagcacat cgacgcccat atggccgggt ttggctattc ccatcagtgc
      181 atttagatta gcgccgtcca agtaaaccaa accgcctttg ccgtgcacaa catcggcgac
      241 cgcttcgata ttcttctcaa aaatcccaag tgtgttcggg ttggtaacca tcaatgccgc
      301 gatatcttcg tccatagctt cggccacggc cgcggcagcg atcacgccct gctcgttgga
      361 tgatatttgc actacgtcat agccgcacaa cgtggagctc gctggatttg tcccatgggc
      421 ggtatccggg acaatgatct tcttgcgcgg atttccctgc gctgaaagat aggcgcgaat
      481 cagcatcaag cctgtcaact caccttgggc gccggccgag ggttgcaggc tgacatcatc
      541 catgccgcca atttccgcga gcatttgctc aagaccgaaa agcaactcta gggcgccctg
      601 attcacggcc ggcggcgcta gcggatgcac gagcgagaag ccgggataac gcgccaccac
      661 ttcattgacc ttgggattat acttcatggt gcaggagccg agcgggtaaa attggctttc
      721 gattgcgtaa ttgcgttgcg ataaacgggt aaaatgacgc agcagctcga gctcgcctag
      781 ctcgggaaaa tccgggatat cgtgacgcaa ataactctcg ggaatactgt ccgtcgccgg
      841 tggtgtttta tccggccatg caaaggcggt tcgtccgcgc gagccgctct cgaagagcag
      901 tggcgcagat tgagcgggct gtggcgggtc agttcgagat cgctcggaca aggcggtcga
      961 tcccttctgt atcgttcatc tcggtgacac atacaagcaa gcaatccggt agctcgggat
     1021 accatctcgc caaagaaatc cctgggataa ttcttcgctc tgaacagcgg cgcatgatct
     1081 cgtcgagctg ttttcccctg actacgaact cattgaaaaa cggcgtccta aagcaacctt
     1141 caagtttaga ctcccggagt aaacgctggt acgcttcatg agcacgtgcg acattgacct
     1201 ccgccaaacg acgcatcccg gtccttccca agaaggacat ataaaccgtt gcagccattg
     1261 tgcacagcgt ctggctggta cagatgttcg atgtcgcctt ttcgcggcga atgtgctgct
     1321 cccgcgtggc cagggtcaaa acatagccgc gccgtccttc gctatcgaca gtctcgccga
     1381 caagcctgcc cggcaggttg cgcatgaatt tccgttggct ggcgaagaaa ccaaaccccg
     1441 gtccgcccag ggccatcggc actcccaagc tttgcccttc gcccacagcg atatcgacgc
     1501 cgaacgctcc aggcggtttt aacagaccga aggccagagg ctccgtggtt gcggtgatga
     1561 gttgagctcc ggcgcgccca cagagctcat gcaggggcgc taggttttcg atgactccga
     1621 agaagttagg atagccgacc agcacgcaca tagtaccttc gttcaactgc cgcgccaggt
     1681 gatcaaaatc ggttgcgccg ctgccgtcaa aaggaatctc ttccaagcta aggtcgctaa
     1741 gattgcgaag ataagtagca atcacagcgc gatactgcgg gtggagcgcg cgagagacta
     1801 aaacccgccg tcggctcgac gattgaatgc gccgcgacat aagaatggct tcagcgctcg
     1861 ccgaagcgcc gtcataaacc ccggcattgg agacttccat cccggtgagc tggcagacca
     1921 aggtctgata ctcgaagaga acttgtaaag taccctggct cacctccggc tggtaaggag
     1981 tatacgaggt cgagaactcc gagcgtgaaa taaccgcatc gacggcgctc gggatgaaat
     2041 gatgatagat gccgccgccg agataaagat cccaatcatt tgcggcggcg ttcttggccg
     2101 caagagcggc aagtcgttgg cggactgtta attcactgag tccgtcgggc aaatggattt
     2161 tggcagtcga acgaaatttt tctggaacat gccggaaaag atcttcgatg ctccctgcgc
     2221 cgatcgcgtc aagcattcgt tttttgtcgg cctcggtgtg cggcgtgtag cgcatacgcg
     2281 aggattattc ctcttcctcg tcatcctctt cggcctcatc gtcctcgtca tcttcttctt
     2341 tttgctgctc gatatattct gcgtattcct cagccgtcat caaatctttc agatcgtcca
     2401 tatcctgcat ttcaactcga atcatccagc catctccata ggcatcatca ttgatgattt
     2461 ccgggctgtc aggaagcgag tcgttgattt ccaaaaccgt tccgctcacg ggcgcataaa
     2521 tatccgacac cgctttgacc gattcaacgg cgccgaacgg atcgtctttt acaatctttt
     2581 cgccgatctc cggtaattcg acgaaaacga tgtcgcccag ttcctcctgc gcgtattcgg
     2641 tgataccaat ggttgctgtc ttaccctcca ccagaaccca ttcgtgttct tttgaatatc
     2701 tgagcccctc aggaatctcc atgaccttcc tccttacact gctcacgccg tgtcagcgcc
     2761 ggtaaaacgg caatggcact atcttggcgc gaactgcttt gttgcggatt tcgacttcaa
     2821 gtatattttc ggcgcgggct tcttccgcct ccacatatcc aagcgcgatc gaccgcccca
     2881 gagtcggtga tcgggtgccg ctggttactt tgccgataca ccgtccctct ttcaacaacc
     2941 gatactcact gcgcgcgatc ccgggttcga tcagctccag gccgacaagc ttgcgtttga
     3001 cccctgcttg tttctgcctt agcaagactt cacaaccgat gaaagagcct ttggaaaatt
     3061 tcaccagcca ttctaatcct gcctctaacg gagtcgtgct gtcgtcgagt tcgtgaccat
     3121 aaagcggata agctttctcc aaacgcaagg tgtcgcgcgc gccaagaccg gccggcacca
     3181 agcccgccgg tgaccccgcg tcgagtagat cgcgccacag cttttcggcg tcctgcgaag
     3241 aacagtagag ctcgaatccg gcttcgccgg tatagcccgt gcgcgcgatc aggcagcgcg
     3301 tgccggcaac ttcgctctcg gcaaagtaga aggtcctgat gtcggagaga gcgatggctg
     3361 ctaacggctg cattattctt tctgccagcg ggccctgtaa tgccaattgc gcataggccg
     3421 cgctgacatt ttcgacttga atgtctccgg tggcatgctg gcggagccat tggaaatcct
     3481 ttccgctatt ggaggcattg acgcaaataa gatagtcatc cggctgtcgc ttgtaaaaaa
     3541 tgacatcatc gatgaccccg ccccgatcat ttaaaagcaa gttatactga gcttgggata
     3601 cttgcatgcg cgcgacatca ttggcggtaa gcctctggca catcgcttcc gcaccggcgc
     3661 cgcatacggc aatctcgccc atatggctga catcgaaaag cccggcccgg ctgcgcaccg
     3721 ccagatgttc ggcgatcaca ccgctgtact ggacgggcat ttcccagccg gcaaagtcaa
     3781 ccatgcgccc gccggcgcgg cggtgagcgg agtataatgg agttcgattc atgtgctccg
     3841 acaagacttc aggagggaag ctggttttgg ctaacgttcg cgctggatcg aatccttggc
     3901 cgccttcaga gtgttataca agagcatgca aatggtcatt gggccgacgc cgccgggcac
     3961 gggcgtgatg gcagatgccc gttctttagc ggtttcaaat tcaacgtcac cggtgagttt
     4021 gccgctgggc aaccgattga caccgacgtc gatgaccacg gcgccaggct tgatccaatc
     4081 gcctcggacc aatcccgcct tgccgatcgc caccactaaa acatccgccc tggctacttc
     4141 gtcgcgcaag ctcgcggtcc gagaatgaca aatcgtcacc gtggcatgct tttcaagcag
     4201 catcagcgct actggtttgc caacgatgtt gcttcggccg acgacgacag cattcttgcc
     4261 tttgagggca cagcccgccg cctccagaag tttcatgacc cccatgggcg tgcagggtcg
     4321 gaagccattg gcgccgatga ccagcattcc ctggctcata ggatggaacc catcgacatc
     4381 tttgaacgga gacacagctt cgagaattct ttccgaacgg atgtgagcag gaaggggcag
     4441 ttgaaccaga atgccatgaa catctttatc tttgttcaaa ctctgaacca gagagagcag
     4501 ttctttttcc gaaacggtgg cgggaaggag atgctcagag gattttattc ccacttcttt
     4561 acaggctttt tctttgttct ttacgtaaat gtgggatgcc ggatcatcgc caaccaacac
     4621 aacagctaat cccggcgcca ttccccaacg ccgttccaac ccgtcaactt cctctttgat
     4681 tctcttctga acttctttgg ccacggcttt gccgtcgatg atcgatgcca tgcctacctc
     4741 ttgaagaagg agagtgtgat ggagaaaaga aacattttag agcagcgacg ttcctattgg
     4801 catgccacgc actaagccaa aggtcaaccc taatcgcggc caggttgccc ggcgaaaact
     4861 catccgtaaa atttgaaatc tcgcgctttt ctgttattcg gacagatagt gcctcttgat
     4921 tgccatggaa agaaaacttc acatacaacg aataaatcct ggaagatgca gtgcgtcaca
     4981 gtaaccgccc tgcgggcgcc cagatcgcgc gagcggactc taagattgca tttatgcccc
     5041 cggccgatct atggttttcg acagatgcca acgtaacttt actgcgcgga caacgaggag
     5101 atttatgaac cagccacgta gtcttgaaga gatcaagaat gacattcgcg ctcgcctggg
     5161 tcaccgagcg ccgtttctac atgccgacaa agccgaagct gaagaagcgc tcggcaggat
     5221 gagttcggtg caaggcgaga tctgggcgca gtcatggaat gaattgggcg cgcgctggga
     5281 agcaaaagca cgggaggccg agagcgccgg caacaaacaa gaagccaagg aaaactatct
     5341 caagtcctac ggctactacg gcattgcgcg ccatcccttt cccagcactc cggggaaaca
     5401 tcacggctat ttaaaaaccc gcgaaatgtt tctcgccgct tcgaagtatt tcgagatccc
     5461 gatcgaacgc gtgtccattc cgtttcaggg caaacaaatt atcggtcatc tgcggctgcc
     5521 caagaaagtg cccgctccac tgattatgca ttggggcggc atcgacaatt ggaaggaaga
     5581 acgtcattcg tttgccgaat cgttcgtaaa agagggctgg ggctgctttg tgatggacag
     5641 tccgggaacg ggcgaatgcc cggtgcttgc cggaccggag gcgcaccagg ttcacaccac
     5701 ggcgttggag tatctgagag aaagaccgga ggtcgataca actcgcatcg ctgtggtcgg
     5761 tgccagcttc ggcggtcact ggtcgaccaa actggcccat ctcgcgcccg ataagttacg
     5821 cgctgcggtc aactggggcg ggggcattca ctacttcttt caacctgagt ggcaagaacg
     5881 atcgcgcaat gccgactctt acctgttcga tctgatcgaa gcgcgtgcca atcttttcgg
     5941 caaacagagt ttcgacgaac tgtgccaggt tatgccggca ctatcgctgc tcgatcaagg
     6001 gtggttggag aagccctgtg caccgttgct gatcgtcaac ggtaaagaag acaagcaggt
     6061 gccacttgaa gatttctatc tgctcttgga gcatggcgat ccgaagacga ttcgcctgtt
     6121 tccgggcggc cacatggggg ctattccgaa tatctttaaa actgtgatca cctggttgca
     6181 ccagagattg gacggttgat atgagtctct atcagctaca gaaactgatc tatgacgtaa
     6241 accgcaaccc ggagcggcgc gacgcctatc gagccgacaa aaccggattt ctcgctcgct
     6301 acgaactaac tcccgaggag caagcggcga tagcagacct ggatgtgcgc aagctctata
     6361 gctacggtgt tcatccgttg ctgttgcgcc cgttcacgtt actgcaccgt gtatccaacg
     6421 aagactacgc caaggcattg gctggattgg agtaaaatat gccgatcgta tttgcctgcg
     6481 ccgcgagtca tgccccagga atgactgcct ggactgaagc cgcgccccaa gagcaacggg
     6541 ataaaattct tggcagctat cacaagcttg ctgagatgct agccgcttca aagcctgatg
     6601 ctctggttgt ggtcagtgtc gaacactggg ctaatttttt cttgaatcag atgccgacat
     6661 tctgcatcgg ccgggcccaa tattatcatg ggccggtgga agaatggttg aaaatttcca
     6721 agacgcgggt gcccggggat gcagctttgg gcgcgcagct gatagaggcg tgtctcgaca
     6781 gcggctttga tccggcgttt tccgatgaac tattgttcga ccatgcgacg atgttgccgc
     6841 tgcactttgt aaatcctggc atgaccattc cagtggtgcc gctgattatc aataccttga
     6901 cgcccccgat gcccacacct aagcggtgtt ttgaattagg ccgggtgctc gggcaagcgc
     6961 tggagcaaca agcaaagcga attgctttga ttgcgacggg cggtctctca cactggcccg
     7021 gcgaagcgaa gatgggcaaa atcaatgtag ctttcgatga gcaattcctc caactcttga
     7081 tcgacggcaa gcgcgctcgt ctggccgaat actcccatgc acagatcgac aaagaggccg
     7141 gttccggcgg tcacgaaatc aggacctgga tcactgtggc agccgcggta gaggattggc
     7201 gggcaagcct gctcgcttac gagccggttc acccgtgggc aacgggctgc ggcctagtgg
     7261 cgttcactgc ataagatttc tcgccacggc agcagtccgc aagcgctcac cgactggcat
     7321 cgagtgtatg gagaagcaaa tggcgcttaa attcagtttg cacagtcttc acggtggcgt
     7381 gaaaagtatc gatgaaattc atcaacgcgc gcaacgcgcc gaagagctag gctacgacgg
     7441 gatatttctt
//
LOCUS       DASUTG010000728         5823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1742771, whole genome shotgun sequence.
ACCESSION   DASUTG010000728 DASUTG010000000
VERSION     DASUTG010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742771"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1333
                     /locus_tag="VF089_16860"
     CDS             236..1333
                     /locus_tag="VF089_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175683.1"
                     /translation="MIINPNNHRFLLIFLAGIALLTGLWAGIARMGWFLPVPNSQFVV
                     VHGPLMVVGFLGTLIGLERAVALQRWWAYAVPTCAGLGAIGALMALPVQVSASLAALA
                     SLFLIAVFITLYRQYPSEHFIIMALSALAWFVGNALWFTDAAVFTIVPWWVGFLVLMI
                     AGERLELSRLRQPSALVRHGFHGCIALVVIGLACSFFDFFTAIRISGFGFLGLAAWLL
                     RYDLAWQSARQPGLPRFMATSLIAGYVWLAMGGILWMVFAQFFSAGPRYDAMLHAIFL
                     GFVFSMIFAHAPIILPTITGLSLPFQNIFYLHAALLHVSLLLRIVGDLSPSASLQQWA
                     GLLNAGAILLFLVNNVRAVRLGARVGSIRKS"
     gene            1490..2647
                     /locus_tag="VF089_16865"
     CDS             1490..2647
                     /locus_tag="VF089_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HEX6175684.1"
                     /translation="MDFNQRPFTVIWETTRACDLACVHCRAGANSQRSAGELTFEEAQ
                     KLIQGIKEFGAPYPLFVLTGGDPAKRSDIFDIISAAREQGLRVAMTPSATPLIDRETV
                     RRFADNGLARLALSLDGNDALTHDSFRGVTGSFKRTLQILDWCREFGLETQIHTTVTR
                     HVLDDLPAIAEMIGRRGIKLWALFMLIAVGRAARPEIRRLNLSAAQFESFFHWLYDLA
                     NAASFDVTPREGYHYRRVLLQRRAAELGVAVADLLADVEKKHSTPSDVARAPQATRIV
                     RAPLGVSDGKGIVFISHQGDVQPSGFLNLVGGNVRRESLAGIYRNSPVFLRLRDSSLI
                     EGKCGVCEFKNICGGSRSRAYAITGNPMRSDPFCVYRPAAWGNRCPTQRRS"
     gene            2704..3288
                     /locus_tag="VF089_16870"
     CDS             2704..3288
                     /locus_tag="VF089_16870"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6175685.1"
                     /translation="MTHATSQDSSPSTHADKPTEILSNEHRLIERVLTAVEKLLQGPV
                     NALEPWKKALDFMRHFADECHHFKEEKLLFPVMEEHGIPIEGGPIGMMLVEHEEGRSY
                     VSAMLMALSLVEGGNEAAKESLVSSAQAYCRLLREHIEKEDEILFRMADEVISADQQK
                     KLLALFAEHEAKQIGAGANEKYLRIALELEDGTG"
     gene            3396..4394
                     /locus_tag="VF089_16875"
     CDS             3396..4394
                     /locus_tag="VF089_16875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6175686.1"
                     /translation="MNPFTIMAMSLLLLFANAPSYGASYGGKTITIVVGYKPGGGYDR
                     YARLIGKYLPKYVPGNPGVIIQNMPGASSVIAANHLFSVAKPDGLTIGTFNNATVIAQ
                     LIKVEGVRFDLAKFSWLGSAASDAVILSVRSDLPHRTADDLRKAKQFVIGTTGPGSST
                     HDFPALLKEFAGFDFKLVPGYSSSADVMLAIERKEVDGRAGSYDSIKPFIDRGLVRPI
                     IRNRTSAPGIEKLPIDENLATTQQGKLVMAVNAVPTQIYRPFVAPPGTAKDAIKMLRD
                     AFANSLKDKDLLAEAEKGKMNIDYVSPEDALKVVSEVLGQPPDVVKVLAKHIKFGE"
     gene            4446..>5823
                     /locus_tag="VF089_16880"
     CDS             4446..>5823
                     /locus_tag="VF089_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6175687.1"
                     /translation="MLDAFIQGLFVVFQWQTFLMMLLGIFLGFWVGLLPGLGGTTTLA
                     LMLPFIYQMTAVEAFAFLLGMHSVVSTTGDITSILFGIPGEGTTAATILDGHAMAKKG
                     EAGRALGAALMSSLVGALIGAFALALAIPVVRPLVLSFGAPELFMLAIVGIAFISSLS
                     GHGGRALLRGILAGLLGLLFALVGQDPQAGVLRFTFGQLYLWNGLDLIPVLVGIFAIP
                     EIIDLAVRGTSIAGELPRENLGKGVWLGVKDTFVHFWLTVRCSLAGTFIGIMPGLGGA
                     VAQWIAYGQAVQTARTTAERAGFGKGDVRGVLGPGAANNSKEGGSLIPTIAFGVPGSS
                     SMAILLGAFFLLGIMPGPDMLDKHLVLTFSMVWTMVVANIITVVLSLFFLNYLAKLTL
                     IRGTVLIPFILFFSFIGAYTSNNHLGDLLVLLAFGFLGYLMIKCGWPRAPLVLGFVLG
                     RIAENNFYI"
BASE COUNT         1133 a         1536 c         1693 g         1461 t
ORIGIN      
        1 cggtaaagtt gggccggctt aatctgctgc tggcattaac cgtgatggta cttggcatca
       61 tgctggtgcg cggcgcgccc tggtaagtgc cggaagttaa ttcgaagcgg atcaccaaga
      121 gtcagatgct tcaaacaact cgatccgact ctagtttagg gtttggtact tcgggatttg
      181 gatttgtttg ggatttcgat attcggattt cggatttggt ctcggtcggg aagcgatgat
      241 tatcaatcca aataaccaca ggttcctgct cattttcctg gcgggaattg cactcttgac
      301 ggggctctgg gcgggcatcg ctcggatggg ctggttcctg ccggtgccga acagtcaatt
      361 tgtagtggtg cacgggccgc taatggtggt gggttttctc ggcacgttaa tcggactcga
      421 acgagccgtc gccttgcagc gctggtgggc ttatgcggtt cccacctgcg ccggcctggg
      481 cgcgatcggg gcgttaatgg ctctgccggt tcaagtaagc gcgtctctgg cagctttggc
      541 cagcttgttt ctgatcgcag tattcatcac gctgtatcgc cagtacccct ctgagcattt
      601 catcatcatg gctttaagcg cgctcgcctg gtttgtcggc aatgccctgt ggtttaccga
      661 cgcggcggtg ttcacaatcg ttccgtggtg ggtcggattc ttggtgctca tgatcgccgg
      721 cgaaaggtta gagttgtctc ggctcaggca accgagcgcg ttggttcgcc acggctttca
      781 cggctgtatc gcgcttgtcg tgatcgggct ggcgtgctca tttttcgact tcttcacagc
      841 tatccgtatc agcggttttg gttttcttgg cctcgccgcc tggctgctgc gttacgatct
      901 ggcatggcaa agcgcccgtc aaccaggctt gccgcgcttc atggcgactt ctctgatcgc
      961 cggatacgtg tggttggcga tgggaggaat tctctggatg gtctttgcgc aattcttcag
     1021 cgctggcccg cgttatgacg cgatgctgca tgcaattttt ttgggattcg tgttctcgat
     1081 gatctttgcc cacgcgccga tcatcttgcc gacgatcacc ggactgagct taccgttcca
     1141 aaatattttt tatctgcacg ctgctttatt gcacgtttcg ctgcttttac ggatcgtcgg
     1201 agatctctct ccatcggctt ctctgcaaca atgggcgggt ttgctcaatg ccggagcgat
     1261 cttactgttc ttggtcaaca atgtgcgcgc ggtgcgcttg ggcgcgcgcg tcggttcgat
     1321 tcggaaaagc taatccgcta tcggctgatt tctcggaaac aagaaacggg gcgcgcttta
     1381 ttcccggtga tagcaaccgg cgaccaaacg aaggcgcttc ggagcgccgc tcgccgcgta
     1441 tcctcgcgca tcgctgacgt ggcatgtatc gtgctgcctc cttcccctaa tggactttaa
     1501 tcagcgcccg ttcactgtaa tctgggagac cactcgcgcc tgcgatcttg cttgcgtgca
     1561 ttgccgtgcc ggagcaaatt ctcaaagaag cgccggggag cttacgttcg aggaagcgca
     1621 aaagcttata caaggcatca aagaattcgg cgcgccgtat ccgctctttg tcctgaccgg
     1681 cggtgatccg gccaaacgct cggatatttt cgacatcatc tcggctgccc gcgagcaggg
     1741 gctgcgggtg gcgatgacgc cctcggcgac accgcttatc gaccgggaga ccgtgaggcg
     1801 gtttgccgat aatggtcttg cccgccttgc gcttagtttg gacgggaacg atgcgctgac
     1861 ccacgatagc tttcgcggcg tcacgggttc gtttaaacgt acgctgcaaa tacttgactg
     1921 gtgtcgagag tttggcctgg aaacgcagat tcacaccact gtcacccgtc atgtgctgga
     1981 tgatctgccg gccatcgccg aaatgatcgg tcgacggggt ataaaacttt gggctctttt
     2041 tatgttgatc gccgtcggca gggcagctcg accggagatt cgtcgcctga atctcagcgc
     2101 agctcagttc gagtcctttt ttcattggct ctacgatttg gcgaacgccg cgtctttcga
     2161 tgtcactccg cgagaaggtt atcactaccg cagggtgtta ctgcaaaggc gagctgccga
     2221 gttgggtgtc gccgtggcgg atttgcttgc tgacgtggaa aagaagcact caacaccgtc
     2281 ggatgtcgca agagcgcccc aggcaacgcg aatcgttcgc gctccgctgg gcgtgagtga
     2341 cggcaagggc atcgttttca tctcacatca aggggacgtg caacccagcg gttttctcaa
     2401 tctggtcggc ggcaatgttc ggcgtgaatc gttagctggc atctaccgta attcgccggt
     2461 attcctacgg ctgcgcgact cctctctgat cgaaggcaag tgtggggtat gtgagttcaa
     2521 gaacatctgc ggcggcagcc gatcgcgggc ctacgcgatc accggaaatc cgatgcgtag
     2581 cgatccgttc tgtgtttacc ggcctgctgc ttggggcaat cggtgtccaa cgcaacgccg
     2641 tagctgaaag accgctcgcg aagtatagat aagatcgccc gtaccaccga aaggaatgat
     2701 cacatgacac acgcaacttc gcaggattct agcccaagca ctcacgcgga taagcccacc
     2761 gagattctta gcaacgagca tcgtctcatt gagcgcgtgc tcaccgcggt agaaaagctt
     2821 ttgcaaggac cggtgaacgc attggagcct tggaagaaag cgctggattt tatgcgccac
     2881 ttcgccgatg agtgccacca cttcaaggag gaaaagctgt tgtttcctgt tatggaggag
     2941 cacggtatac ccatagaagg gggacccatc ggcatgatgc tggtggaaca tgaagaagga
     3001 agatcttacg tcagcgccat gctgatggcg ctctcgttgg tggaaggagg caacgaggcc
     3061 gccaaagaga gtctcgtcag tagcgctcag gcctactgtc gtctactgcg cgagcatatt
     3121 gaaaaggaag acgagatcct ttttcgcatg gcggacgagg tgatttccgc cgaccaacag
     3181 aaaaagcttt tggccctttt cgctgagcac gaagccaaac aaatcggggc gggagcgaac
     3241 gaaaaatatt tgaggatcgc gctggaattg gaagacggaa cgggataaac gcaacttagg
     3301 agttcgaatc ggtcgcgcat cagtgggggc cttcatgagg tcatcagcac atcgatctgc
     3361 taaccgaatg aaggcgtatt caaaaaggag cttttatgaa tcctttcacg atcatggcta
     3421 tgtctctgct cctgttgttt gcaaacgcgc cctcctatgg cgcttcttat ggaggcaaaa
     3481 cgatcacaat tgtcgttggc tataaacctg gcgggggcta cgatagatat gcgcgactga
     3541 tcggcaagta tctccccaaa tacgtcccgg ggaatcccgg cgttatcatt cagaacatgc
     3601 cgggagcgag cagcgtgatt gcggcgaatc atctttttag cgtcgccaag cccgacggtc
     3661 tgacgatcgg gacttttaac aatgctacgg tcattgccca gctgatcaag gttgaggggg
     3721 tgagattcga tttagccaag ttttcttggt tgggcagcgc tgccagcgat gcagtgatcc
     3781 tcagcgtgcg ttccgacctt ccgcacagga ccgcagacga cttgcgaaag gccaagcagt
     3841 ttgtgatagg caccaccgga cccggaagtt cgacccatga cttccctgcg ctccttaagg
     3901 agttcgcagg ttttgacttc aagcttgtcc ccggttattc gtcgagcgca gatgtcatgt
     3961 tggcgatcga gcgtaaagag gtagatggcc gggcggggtc ttacgattcg ataaagccct
     4021 tcatcgaccg cggtctggtg cgtcctatca ttcgaaatcg gacctcggcg ccgggaatcg
     4081 aaaaattacc gattgatgaa aatcttgcaa cgactcagca gggaaagctt gtcatggccg
     4141 tgaacgctgt cccgacgcaa atctaccggc ctttcgtagc gccgccggga acagcgaagg
     4201 acgcgataaa aatgctccgc gatgcatttg ccaactcact caaggataag gatctccttg
     4261 cggaagccga aaagggcaag atgaacatcg attacgtgtc gccggaagat gctcttaagg
     4321 ttgtctctga ggtgctaggt cagcctccgg acgtcgtcaa ggtgctggcg aaacacatca
     4381 agtttggcga atgaggctgt aaacgaccta gatactcgga gtagggaggc ttgccgtctt
     4441 aacttatgct ggatgcgttt atccaaggac tgttcgtagt cttccaatgg cagacttttc
     4501 tcatgatgct gttgggcatc tttctgggct tctgggtggg actgctgcca ggtctcggcg
     4561 gcacaaccac cctggccctg atgcttccgt ttatttatca gatgacggct gtcgaggcgt
     4621 ttgcttttct cttggggatg cactcggtgg tctctaccac gggagatatc acgtcgattc
     4681 tttttggaat tcccggcgag ggcactaccg cagcaacgat tttggacggt cacgcgatgg
     4741 ccaaaaaagg cgaagccggc cgagctttgg gggccgcgct catgagttcc cttgtcggcg
     4801 ccttgattgg cgcctttgcc ttggcccttg cgatcccggt cgtgcgcccg ctggttcttt
     4861 catttggagc gcccgaactc ttcatgcttg ccatcgtcgg cattgccttt atctcgtcgt
     4921 tgagcggaca cgggggacgg gccctgctgc gcggaattct cgccgggctt ttgggacttc
     4981 tattcgctct cgtgggccaa gaccctcaag cgggggttct gcgcttcact ttcggccagc
     5041 tctatctatg gaacgggctc gacttgatcc ctgttttggt gggcatcttc gctattcccg
     5101 aaatcattga cctcgccgtc agagggacct ccattgcggg cgagctgccg cgagagaacc
     5161 ttggcaaagg cgtttggctt ggggttaaag acacgttcgt gcatttctgg ctaacggtcc
     5221 ggtgcagcct cgccggcacc ttcattggaa tcatgccggg attgggtgga gcggtggcgc
     5281 agtggattgc gtacgggcag gcggtgcaaa ccgcacggac cacggcggag cgggcaggat
     5341 tcggcaaagg agatgtccgg ggtgtcctgg gtccgggcgc ggccaataat tccaaagaag
     5401 gtggatccct catccctacc attgcgtttg gcgttcccgg cagctcttcg atggcgattc
     5461 tcctgggagc tttttttcta ctcgggatca tgccgggacc tgacatgttg gataagcatc
     5521 tggttctcac cttctctatg gtctggacaa tggtagtggc caatatcatc accgtggtcc
     5581 tcagcctttt ttttctgaat tacctggcca agctcactct gatccgcggg actgtgctta
     5641 ttccgtttat cctgtttttc tctttcatcg gggcctacac ctccaacaat catctgggag
     5701 atttgctggt tttactcgcg ttcggcttcc tcggttatct catgatcaaa tgcggctggc
     5761 ctcgagcccc gctggttctc ggttttgttt tgggcaggat agcggagaac aatttttata
     5821 ttt
//
LOCUS       DASUTG010000729         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1446856, whole genome shotgun sequence.
ACCESSION   DASUTG010000729 DASUTG010000000
VERSION     DASUTG010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446856"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VF089_16885"
     CDS             <1..463
                     /locus_tag="VF089_16885"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEX6175688.1"
                     /translation="KGQIKLVEIPSQKETNDNIVAYWIPRSMPAVGQPLDLAYRINFQ
                     SEEPLDAASGRVTATRVGVGDKEDLKRIILDFEGGKLKSLPDTAPVKAVIAIAGDAQL
                     VQQSTVKNPITGGWRAAFQVKPTKDKPLQVRVFLRNENDILTETWSYLLEP"
     gene            460..>2637
                     /gene="mdoH"
                     /locus_tag="VF089_16890"
     CDS             460..>2637
                     /gene="mdoH"
                     /locus_tag="VF089_16890"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX6175689.1"
                     /translation="MSKNIHRPSFSSADNQSLSEIPAAGNGICEVNTSANSDVVVGWQ
                     SERDTPSGKRDFNKRRQLHHRWAKGGRDPRLAQIDLNAVRGNIHALAMPELKRLSMLP
                     NRVERNPLVSLWRRLRGNTSNNEMESVCSGSIEGPVVGWRATATSRRLALIALIITQT
                     AIATWSLSNTFPYPWLKASEIAILVLFAILFSWISLGFWSAVAGFWMLWRRKSEFTCA
                     DLPVDERQPLLSRTAVLMPICNEDVTRVFAGLEASYRSLAETPQFASFDFFVLSDTGD
                     PVRQAEEEIAWAQLCAGVQGFGRIFYRHRRNNVRRKSGNIADFLRRWGRDYDYMIVFD
                     ADSVMAGETVVRLAQMMEQYPQAGIIQTAPTTVNCDSLFGRLQQFASRAYGPMLTAGQ
                     RFWQLGEGYYWGHNAIIRTAPFIKHCGLSRLPGHAPLGGEILSHDFVEASLMGRAGWE
                     VWVVHDLGGSYEESPPTLADELKRDRRWCQGNLQHLRLLLADGFRGGHRAILAMGVMA
                     YASALLWFVFLILNTVELAAQTVLPLVYFSSEPSLFPLWPSWHPEWAVALLTTTAILL
                     FLPKLLSLLVIIKDRQTHLFGGFLPLCVSICLELIVSTLLAPIRMWFHSQFVVVTLMG
                     RQIKWGPQFRTDNETRWRDAIRLHGVATLLAFGWVFGMYRLNPAVSVWLLPVGISLLL
                     AVPLSVYSSRLSLGRLTRRWRLFLIPEEIRSPQVIKHLQATLSK"
BASE COUNT          540 a          679 c          769 g          649 t
ORIGIN      
        1 taagggacag ataaagctgg tggaaatccc gagccaaaaa gagaccaatg acaacatcgt
       61 ggcttactgg atcccgcgca gcatgccggc ggtcggccaa ccgctggatc tcgcctacag
      121 gattaacttt caaagcgaag aacccctcga tgctgcgtca ggccgggtaa ccgcgacccg
      181 tgtcggggtc ggcgataaag aggatctaaa gagaatcatc ttagattttg aaggcggtaa
      241 gctcaaaagt ctgccggaca cggctccggt gaaagcggtg atcgcgatcg ccggtgacgc
      301 gcaattagtt cagcagagta cggttaaaaa tcctatcacg ggcggctggc gcgctgcctt
      361 tcaagttaaa ccaacgaagg acaaaccgct gcaggtgcgc gtatttctac ggaatgaaaa
      421 cgatattctc acggagacct ggagctattt gttggagcca tgagcaaaaa tatccatcgg
      481 ccgagtttct caagcgccga taaccaatca ttgtctgaga ttcccgcggc gggcaacgga
      541 atctgcgagg taaacacctc ggcaaacagc gatgtcgttg tcggttggca aagcgagcgc
      601 gatacgcctt cgggaaagcg cgacttcaac aaacgaaggc agcttcatca cagatgggct
      661 aaaggtggac gcgacccccg cctcgctcaa atcgatttga acgcggttcg cgggaatatc
      721 catgccttgg ccatgcccga gcttaagcgt ttgtcgatgt taccgaatcg ggtagaacgt
      781 aatccgctgg tgagtctatg gcgccggctg cgcggcaaca ccagcaataa tgagatggaa
      841 agcgtttgct caggctcgat cgaaggacct gttgttggtt ggcgcgctac tgcgacttct
      901 cggcgtctgg cgctgatcgc tttgataata actcagacag cgattgccac gtggtctctc
      961 agcaacacct tcccttatcc atggttaaag gcgtcagaga tcgccatcct agtgttgttc
     1021 gccattctgt tttcgtggat ttcgctgggc ttctggagcg cggtggccgg attctggatg
     1081 ctctggagac gcaaaagcga atttacctgt gctgatttgc cggttgatga gcggcaaccg
     1141 ttgctttcgc gtactgccgt cctcatgccc atctgcaatg aagatgttac tcgtgttttt
     1201 gccggcctgg aagcgagcta tcgctcactt gcggagacac cgcaatttgc aagttttgat
     1261 tttttcgttc tcagcgatac cggcgatccg gtcaggcaag ccgaagagga gattgcgtgg
     1321 gcgcaactct gtgccggggt ccaaggcttt ggcaggattt tttatcgcca caggcgtaat
     1381 aatgtgcggc gtaagagtgg taacatcgcc gatttcttgc gccgctgggg acgagactac
     1441 gactacatga tcgtctttga cgccgatagc gtcatggccg gcgaaactgt cgtccggctg
     1501 gctcagatga tggagcagta cccccaagca ggaatcatcc agacggcccc gacgaccgtt
     1561 aactgcgatt cacttttcgg acggttgcag cagtttgcga gccgggccta tggtcccatg
     1621 ctgacggcag gccagcgttt ctggcagctg ggtgagggtt attattgggg ccataatgcc
     1681 attatacgca cggcgccatt catcaaacat tgcggcctat ctcgtctgcc ggggcatgcg
     1741 cctctgggcg gagaaattct aagccatgac tttgtcgagg cgtcgctgat gggccgggct
     1801 ggttgggaag tctgggtggt tcatgatcta ggcggcagct atgaggagtc gccgccgact
     1861 cttgccgatg aactcaaacg tgaccgccgc tggtgtcaag gtaatctgca gcatttgcgc
     1921 ctactgctcg ctgatgggtt tcgcggcgga catcgggcca ttttggcgat gggcgtcatg
     1981 gcttatgctt cggctttgct ctggtttgta ttcttgatcc tcaatacagt ggagcttgcg
     2041 gcgcagacgg ttctgcctct ggtctacttc tctagtgaac cgagcttatt tccgctctgg
     2101 cccagttggc atcccgaatg ggcggtagcc ttgctgacga ccacggcgat actgctgttc
     2161 ttgccgaagc tcttgagtct cctggtaatc atcaaagatc gccagacgca tctttttgga
     2221 ggtttcctgc cgttgtgcgt gagtatctgt cttgaattaa tcgtgtcgac tttgcttgcg
     2281 ccgatccgaa tgtggtttca tagccaattc gttgtcgtca ctctgatggg caggcagata
     2341 aaatgggggc cgcaatttcg cactgataat gaaacgcgct ggcgcgatgc catccgcctg
     2401 cacggtgtcg cgacgttgtt ggccttcggt tgggtgttcg gcatgtaccg gttgaatcca
     2461 gcggtttcgg tctggctcct gccggttggt atctcgctgt tattggcagt accgttatcg
     2521 gtttactcca gtcgtctttc cttggggcgg ctgacccgac ggtggcgact cttcctgatt
     2581 ccagaggaaa ttcgttcgcc gcaggttatc aagcatcttc aggcgacatt aagtaag
//
LOCUS       DASUTG010000730         6151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2189224, whole genome shotgun sequence.
ACCESSION   DASUTG010000730 DASUTG010000000
VERSION     DASUTG010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189224"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..5667)
                     /locus_tag="VF089_16895"
     CDS             complement(67..5667)
                     /locus_tag="VF089_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6175690.1"
                     /translation="MTEPLAYTIVNVDDDDAGRYAKSRILRRAGYRVLEASTGAGGLA
                     LVAEVKPQLLLLDVDLPDMSGLEVCRSIKANPSTSGILVLQISASRITAEDRVSGSEG
                     GADAYLTEPVQADELVATSRALLRLHSREQENRELLAALRYSEAQFRASFELAGVGKT
                     QIDATTGKFIHVNQCYCDLVGYTKSELLDLRPSDLIHPDDRRHEAELVAALLRGNTEE
                     LTGEKRLVRKDGRIIWASINAKLIRDANGAPERTISSVVDISDGKHAEERARRQNERL
                     ALLAQVSEQLLTAHDPAVMIREVFETVRQHLALDTYFNYRVDSSGSALVLESRGDADP
                     GALPASQPLHIGKLLHESPAHLRQEVILEALKKVDDQQAKSARASGFSAYAGHPLLVA
                     ERLIGTFCFASKRRDGFDRDEIDFMRTICHYVAMAQERLRLLAESRQQVERLEQSESR
                     LRLAVDGAGMGIWDVDLRTDTLVWNRELYSLLGYDSDAGPVTRQMWRSRIHPEDFAGV
                     AQSMERAKSSRSLYCLEHRIIRADNGEIRWLAPFGRFLYDSAGAAVRFLGIVFDFTDR
                     KRAEDAFREAAERLRLAQVSAHLGIFDWNARTNDLAWTPEQEMVYGVAARGMTRYEHW
                     QRLVHPDDLARVETERAEAIRQRQPFTLEYRILHPSGKIHWIASKGQGWYDASGELVR
                     VLGVNIDITETKRAEEELRHQKQLLETITDNAPSMLFMTDPMGRVTFANPAATHLTGY
                     DEQELIGQVLHEKIHHSRPDGSPYPISDCRLHGVLPFEERITGHEEMFVRRDGSFFSV
                     RCAASSIIQDGVPVGTVIEGQDITKEKKAERALRDSEERMRMLSNHVPVFVWACRADG
                     WCYSLNDRWYEYTGFKPEQALGVGWLDALHPDDRERALSKWEQAVAQGDGYEMELRYR
                     RHDGEYRWFIAKALPVKDAGGLITDWFGTSLDIHDRKSWEQSLAHLAAIVTSSQDAVI
                     SFSRDSKIVTWNRAAEKLFGWSAHEALGQTAAMYVPSEYSEESAWISETLQRGEAIDQ
                     LETVRRRKDGTRVDVSLTISPIKVDQEVIGVSVTARDITERKRIEQQREQQSRLLDLS
                     LDAISVWSVTQGIEYWNEGAEKLYGYSAEEATGRSMQDLLKASQSVSLQEIEDQIRRE
                     GEWEGRISHVSKGGGQVAVLSRQQRIARPGGEVILEVNRDITILEEAEQAVAEAAAHL
                     KAIVETAVDGIITIDENGFVASVNPAVEKMFGYSARELIGRNLSMLMAEPYRSEHDEY
                     LNHYRQTGERKVIGTGREVRGRRKDGSEFPVDLGVSETMIGARRFFTGLLRDATSRKQ
                     VEQALIDAKEAADTASYAKSEFLANMSHEIRSPLTGVLGYAEILLSRLKDPNDLESVR
                     TIKQSGQYLLQIINDILDLAKIEAQGVELDREMIYLPVFLSEVYALMEVNAKQKALPL
                     FLKYDGFIPQKIESDSKRLRQILINLLSNAVKFTDYGKVELATRFLRPQSQLQFEVSD
                     SGIGMTEEQQRKLFHPFTQGDSSVTKVYGGTGLGLAITKRLVAALGGAIAVESSPGRG
                     STFRVTIPVQVVSDSYYGSSTAHESRGPGRLLAEKMAARVLICEDQPEIRRLMGCFIE
                     EAGGRATLLDSGEAAVDVVQQAGNDFDVVLLDIQMPRLDGYEVARRIRQLGFRKPVIA
                     VTAAAMPSDREKCLEAGCDDYLPKPIEFEKLLSMISRYTAKPVTDKAELNRLERQPGA
                     DTSPAGDSLPVSSTPKNWRILVVDDRPVALNATSRLLELEGHTVRSASTGHAAIRVAR
                     EFAPDLVLLDISLPDISGYEVFRRLKASESLARTVFVALSGHGYEERIRARQVGFDDY
                     LMKPFDIKEVDRLIAVNLAQDRRL"
     gene            complement(5711..>6151)
                     /locus_tag="VF089_16900"
     CDS             complement(5711..>6151)
                     /locus_tag="VF089_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175691.1"
                     /translation="QNGKAQPCILIIDDEPVDRYILVRLLEQQPCIIEQAASGFEALA
                     LLREIKPDLIFLDLNMPGLSGYEVLNQIKANPATSDIPVAVVTSAEIGAHQRQQLEGK
                     AYALMNKVDLSAKAVDDLLAAATAPAALQAEVVDSHNAEASGNG"
BASE COUNT         1287 a         1851 c         1655 g         1358 t
ORIGIN      
        1 gagaaaggat aggtgaagaa tcgagtgcaa gagatgacta ggaatcggaa ccttaggaag
       61 cagatcttac aatcgcctgt cttgagctaa gttaaccgca atcagcctat cgacttcttt
      121 gatatcgaaa ggcttcatta aatagtcgtc aaacccaact tggcgggcgc gtatacgctc
      181 ttcgtatccg tggcccgaca acgccacaaa cacggttcgc gcgagtgact cgctcgcttt
      241 cagtcgccgg aacacctcat atccgctaat gtccggcagg ctgatatcca acagcacaag
      301 atccggcgca aactctctgg cgacgcgaat tgcggcatgt cccgtcgagg cgctgcgaac
      361 cgtatgacct tccagttcaa gcaaacggct ggtggcgttc agcgccactg gacgatcatc
      421 aacgaccagg atgcgccagt tttttggcgt cgatgaaact ggcaacgaat cgccggcggg
      481 cgaagtgtcc gcgccgggtt gccgctcgag ccggttcaac tcagctttat ctgtgaccgg
      541 cttggcggtg tagcgcgaga tcatcgataa caatttttcg aattcaatag gtttcgggag
      601 gtaatcatca caaccggcct ctaagcactt ctcccggtcg cttggcatgg ccgccgccgt
      661 tacggcaatg acgggtttac gaaatcccaa ctgccggatt cggcgggcaa cctcataacc
      721 gtcgagccga ggcatttgaa tgtctagaag aaccacgtcg aaatcattac ccgcttgctg
      781 aaccacatcg accgcggcct cgccgctatc gagcaaggtc gctcgaccac cggcctcctc
      841 gataaaacag cccatgaggc ggcggatctc cggttggtct tcgcaaatca atacccgtgc
      901 agccatcttc tcggccaaca agcgccccgg gccgcggctc tcatgggccg tggaggaacc
      961 atagtacgag tcggagacta cctggaccgg aatcgtcacc cgaaacgtgc tgccgcggcc
     1021 gggtgaactc tcgaccgcaa tagcaccgcc gagggcagcg accaaacgct tggtaattgc
     1081 caacccaagg cccgtgccgc catagacttt cgtcaccgaa ctatcgcctt gtgtgaacgg
     1141 gtgaaataac tttcgctgct gttcttcagt catgccgata ccagagtcgc tgacttcaaa
     1201 ttgcagctgc gactgcggtc gtagaaaacg cgtggcaagc tcgaccttgc cataatcggt
     1261 gaatttgacc gcgttgctca acaagttgat caggatctgg cgcaaccttt tcgaatcgct
     1321 ttcgattttt tgcggaataa agccatcgta tttcaaaaat agcggcaatg ccttttgttt
     1381 cgcattcact tccatcagtg cataaacttc gctcaaaaac accggcaaat aaatcatctc
     1441 tcgatcaagc tccactccct gtgcttcgat tttggcgaga tctagaatgt cattgatgat
     1501 ttgcaacaga tactgaccgc tttgcttgat cgtgcgcacg ctctcgagat cgttgggatc
     1561 cttcaaacgg cttagtaaaa tttcagcgta tcctagaact ccggtcaacg ggctgcgaat
     1621 ctcatgactc atgttggcga ggaattcact cttggcatag ctcgccgtat cggccgcctc
     1681 tttggcatct atcaacgcct gctccacctg cttgcggctt gtggcgtcac gcagcaatcc
     1741 ggtgaaaaac cggcgcgcgc cgatcattgt ttcgctaacg cccaaatcca ccggaaactc
     1801 gctgccgtcc ttgcggcggc cccgcacctc gcgaccagtg ccgatcacct tgcgttctcc
     1861 cgtctggcga tagtgattca agtactcgtc gtgttcggac cggtacggct cggccatgag
     1921 cattgagaga ttgcgcccga tcagttcgcg cgcggaataa ccgaacatct tctcgaccgc
     1981 cggattgact gaggcgacga agccattctc atcaatggtg atgattccgt ctacggcggt
     2041 ctcgacgatc gcctttaaat gagccgccgc ctcagccact gcctgctccg cttcctcaag
     2101 aatcgtgatg tctcggttga cctcgagaat gacttcgcca ccgggtcgcg caatccgctg
     2161 ctgccggctg agaaccgcta cctggccgcc gcctttgctg acgtggctga tccgcccttc
     2221 ccattcgcct tcacggcgaa tctgatcttc gatctcctgc agggacaccg actggctagc
     2281 ttttaataga tcctgcatcg accggccggt ggcttcctcg gcggaatagc cgtacagttt
     2341 ttctgcgcct tcattccagt actcgatacc ctgagttacg ctccagacgc tgatagcatc
     2401 caatgacagg tccaagaggc gcgattgctg ctcgcgttgt tgctcgattc tcttgcgctc
     2461 ggtgatatcg cgcgcagtca cagacacgcc tatgacttct tgatcgactt tgatcggtga
     2521 gatggttaaa gagacatcca cccgggtgcc gtccttacgc cggcgtaccg tctctaactg
     2581 atcgatggct tcgccgcgtt gcaaggtttc tgatatccac gcagattctt ccgaatattc
     2641 ggagggcaca tacatcgcgg cggtttgacc caacgcttcg tgagccgacc agccgaaaag
     2701 tttctctgcc gcccggttcc acgtgacgat ctttgaatcc cgtgaaaaag agatgacggc
     2761 gtcctgcgat gatgtgacga tggccgcgag atgagcgagg ctctgttccc aggatttgcg
     2821 gtcatggatg tccaagctgg tgccgaacca gtcggtgatc aggccgccgg cgtccttaac
     2881 cggaagagct tttgcgataa accagcggta ttccccatcg tgacgccggt aacgcaactc
     2941 catctcgtaa ccgtcgcctt gagcgacagc ctgctcccat ttcgagagcg cccgctcccg
     3001 atcgtctgga tgtagagcat ccaaccagcc gacaccgagc gcctgttccg gcttgaaacc
     3061 ggtgtactcg taccaccggt cattgagcga atagcaccag ccgtcggcac ggcaagccca
     3121 gacgaaaacc ggcacatggt tggacagcat gcgcatccgt tcttcactgt cacgcagcgc
     3181 gcgctccgct ttcttttctt tggtgatgtc ttggccctcg atcacggtgc cgaccgggac
     3241 tccgtcttga ataatcgagc tggcagcgca acgcaccgaa aaaaagctcc cgtccctgcg
     3301 cacgaacatc tcttcgtggc cggtaattcg ctcctcgaac ggcagcaccc catgaagccg
     3361 gcagtcgctg atcgggtacg gcgagccgtc gggccggctg tgatgaattt tttcatgcag
     3421 aacctggccg atcagctctt gctcatcgta gccggtcaga tgcgttgccg ccggattggc
     3481 aaaggtcacg cgtcccatcg ggtccgtcat aaacaacatc gatggcgcgt tatcggtgat
     3541 tgtctctagt agttgcttct ggtggcgcag ttcttcttcg gctcgtttgg tttcagtgat
     3601 gtcgatgttg acgccaagaa cccggacgag ctcgccgctg gcgtcgtacc acccctgccc
     3661 cttcgaagcg atccagtgaa tctttccgga aggatgcaga atacgatatt ctagagtgaa
     3721 cggctggcgc tgccgaatgg cctcagcacg ttcggtctcc actcgcgcaa gatcgtcagg
     3781 atgaaccaaa cgttgccagt gctcgtaacg cgtcatgccg cgcgccgcca ctccatagac
     3841 catctcttgc tcaggagtcc atgctaagtc gttggttcgc gcattccagt caaagattcc
     3901 aagatgagcg cttacctgcg ctaaacgaag ccgttccgca gcctcgcgga aggcatcctc
     3961 ggcacgtttt cggtcggtga agtcaaacac gatgccaaga aaccggaccg ccgcgcccgc
     4021 gctatcgtag agaaaacggc cgaacggcgc gagccagcga atctcgccat tatcggcgcg
     4081 tatgatgcgg tgctccagac agtagagcga gcgggacgac ttagcccgct ccatagattg
     4141 cgcgacaccg gcgaaatcct cagggtgaat acgcgaacgc cacatctggc gggtcacggg
     4201 tccggcatcc gaatcgtatc ccaagagcga gtagagctcc cgattccaga ctaaagtgtc
     4261 ggttcgcaaa tccacatccc agatacccat gccggcgccg tcgaccgcca gacggagcct
     4321 gctttcgctt tgctccaacc gctctacctg ctgccgtgac tccgccagga gacgcaaacg
     4381 ctcttgcgcc atcgccacgt aatggcaaat cgtgcgcatg aagtcgattt cgtcgcggtc
     4441 gaaaccgtcg cgcctcttgc tcgcgaaaca aaatgtgccg atcaagcgct cggcaaccag
     4501 cagaggatga ccggcataag ccgaaaagcc cgatgcgcgg gcagactttg cttgttgatc
     4561 atcgaccttc ttgagtgctt ccaggatgac ttcctggcgt aaatgagcgg gactctcgtg
     4621 aagcaacttg ccgatatgaa gtggctgcga tgcgggcaac gcacccgggt ccgcatcgcc
     4681 ccgggattcc agcacgaggg cggaaccgga gctgtccacc cggtaattga agtaggtatc
     4741 caatgccagg tgctgtcgca cggtttcgaa gacttctctg atcattacag cggggtcatg
     4801 cgcggtaagt agttgctcgg aaacttgcgc aagcaacgcc agtcgttcgt tctgccggcg
     4861 cgctcgctct tcggcgtgct tcccgtcgct gatgtcgacg acagacgaga ttgtccgttc
     4921 cggcgcgccg ttggcatcgc gaatcaactt ggcattgatg gacgcccaaa tgatgcgccc
     4981 gtctttgcgc accagccgtt tctctccggt cagctcctcg gtattgccgc gcaataaagc
     5041 tgcaaccaac tcggcttcgt ggcggcgatc gtcgggatga atcaggtcgg aaggtctcaa
     5101 gtccaacagc tctgatttag tgtaaccgac cagatcacag taacattgat tgacgtggat
     5161 gaattttccc gttgttgcgt cgatctgcgt cttgccgaca ccggccagtt cgaagctggc
     5221 acgaaactgc gcttcactgt agcgcaaggc agccagcaat tctcggtttt cttgttcgcg
     5281 gctgtgcaga cgcagtagcg cgcgcgaggt tgcgaccagc tcgtcggctt gtaccggctc
     5341 agtcaggtat gcatcggctc cgccttccga gccagacacg cgatcttctg ccgtgatcct
     5401 ggacgctgaa atctgcaaaa cgagaatacc ggaggttgag gggttggcct taatgctgcg
     5461 gcaaacctcc agcccgctca tatccggcaa atcgacatcc agaaggagca gttgcggttt
     5521 tacctcggcc actaaagcaa gaccaccagc gccggtggaa gcttccagca cccggtagcc
     5581 ggcgcggcgc aggatccggc tcttggcgta acggccggcg tcatcgtcat ctacgttgac
     5641 aatagtatag gccaacggtt cagtcatgag cgtatctcat gcgcatgagg gccgattctg
     5701 ctgtggcgcg tcagccgttg ccgcttgcct cagcattgtg cgagtcgacc acttctgcct
     5761 gtagggccgc aggcgccgtg gctgccgcaa gcaaatcatc gaccgctttg gctgagaggt
     5821 caaccttatt catcagcgcg taggcctttc cttcgagttg ttgacgctga tgcgctccga
     5881 tctcggccga tgtcaccacc gccaccggaa tatcgctcgt agcgggattc gccttgatct
     5941 gattcaaaac ctcgtagccc gagagacccg gcatattcag atccagaaaa ataagatccg
     6001 gttttatttc tctcagcagt gcgagagctt caaagccgct tgccgcctgc tcaatgatgc
     6061 agggctgctg ctccagcagg cgcaccagta tgtagcgatc caccggctcg tcgtcgataa
     6121 tcaagatgca aggctgcgcc ttgccgtttt g
//
LOCUS       DASUTG010000731         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_261441, whole genome shotgun sequence.
ACCESSION   DASUTG010000731 DASUTG010000000
VERSION     DASUTG010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261441"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1158)
                     /gene="nhaR"
                     /locus_tag="VF089_16905"
     CDS             complement(259..1158)
                     /gene="nhaR"
                     /locus_tag="VF089_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEX6175692.1"
                     /translation="MEWLNYHHLFYFWNVARCGSVSRAGEELHLTQATVSAQVKSLEV
                     AIGEKLFRKSGRHLTLTDMGKVVFRYAEEIFSLGRELTGALKGRAGGRVARLTVGVAD
                     VLPKLVAYKLIEPALGLKESYRIICREGSNADLLASLAVHDLDVVLTDAPIDPAVNVK
                     AFNHLLGECGLILFATQKLVNTYRRGFPRSLDGAPFLLPTHNTTVRRSLDQWFDSEKI
                     RPMIIAEFEDSALLKVFGARGLGLFVAPSIISVEVQREYDVKALGRVGNIRERFYAIS
                     LDRRLKHPAVVAISEAARVRLSD"
     gene            1276..1707
                     /locus_tag="VF089_16910"
     CDS             1276..1707
                     /locus_tag="VF089_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175693.1"
                     /translation="MSAIRNLFSSEDSGRLFEPDTVLSAQFYATYKGGPYKQPELRLL
                     AAVLEDAISCLTVKARPATARQRKQFDDAWQWFAAEDDDSEWIFSFKNICDALGIDPA
                     YLRRGLIRWAAATELGSAGDLSGVRSAASARPTKIRLRAGS"
     gene            2061..3593
                     /locus_tag="VF089_16915"
     CDS             2061..3593
                     /locus_tag="VF089_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175694.1"
                     /translation="MSPKFRNLCLLMSLTSIILFTGAERPSAQTRVSIGVTETIETLN
                     PYGDSVSLLYGIWSEITGPFCTYNYEKGDFEGRLAERWKVENPSTWLFYLNKSYRFND
                     GSEVTADDVVHSMMNRVIKDPQSKQKASVAGPIIKAEAVDKHTVKFTTDKPSAPLLGF
                     VCDRLIVTSKAMFDKYGRDVADKEHMLGAGPYRLKELIPGQRMVIVKRSDHPEAKKHP
                     NAPDEIVYRVMRETEQRVTALLNGEIQIAQFIPPHLRHRVEKSPNLKITATDSVEIMF
                     LAMQPKPPFDKKEVRQAVCHAINRDQIISTLLEGFASRLDGPIGPGQYGYDPNLKPKL
                     SYDPERSKKLLAQAGYPNGVDVELQTPVGRYTLDKQITEAMIPMLNAAGFRAKLSTPE
                     WPTLWANVQKGTVPFYYMGRGSVQDPSAALHQYFHSKETPRIGYSNPKLDELLDKEQA
                     EFDPKKRREYLSQAMSLITEEAPACFMWRHKLLWGMNNRIDYKPLPDARIFGMHIKVA
                     KK"
     gene            3648..4526
                     /locus_tag="VF089_16920"
     CDS             3648..4526
                     /locus_tag="VF089_16920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175695.1"
                     /translation="MTGEKFIDVAGVRTRYFEKGDGPVVVLFHGGHFGSNDAADCAED
                     WALNFDGLAKWFHVYAVDKIGQGHTDNPKTDLDYTISTVVQHAYFFLKALDLRNVHPV
                     GHSRGAYLVARLTLEHPELFRSCILADTNTLAPGISKNETVMANPPLPRLSRESQRWV
                     LQRYSFAFEHITDEWLDAMMRVAALEKYKEAVTKMETAGLRTTRFLPHLSREKDETLG
                     WIRERGLAKPTLLVWGYNDPTATLDQGLGLFDLIARSTPDSRMKIFNRAGHFTYREHP
                     VEFNESLRSFIQRNSG"
     gene            4576..5577
                     /locus_tag="VF089_16925"
     CDS             4576..5577
                     /locus_tag="VF089_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013368602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175696.1"
                     /translation="MTSYLLHRVTGTIPVLLLISLLVFLLIHAAPGDPTLLLLGEETN
                     AAEVARAKERWGLDRPLYVQYLKFLNSAVAGDFGKSFKYGEPVTSVIKSRLPATIELA
                     VFSIIIATCLAIPLGVWAGSRPNSWLDNFGTTFGLFGISMPSFWLAIMLILLMAGVLN
                     LLPTSGRSTYGIAGPEQTGFYILDSLLQQNWQAAWDALRHILLPALALGVNMLGILMR
                     VTRSAILEVMNEEYVTTARAKGLAERRVVWRHVTSNALIPVITVVGLELGTLLSGSII
                     VETVFAWPGSGSLLISALNSRDYPLVTGLVMSYTVAFVIINLVIDGLYAVIDPRIRF"
     gene            5546..>6026
                     /locus_tag="VF089_16930"
     CDS             5546..>6026
                     /locus_tag="VF089_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175697.1"
                     /translation="MRSLTLESASEKTPGAISSALGRLHAKAFSGGSIVLIFILLGIF
                     GPLLAPHDPNKQELTAMMKPPQGIGGAHVLGTDNLGRDILSRIVYGARVSLLVAFAVV
                     FVSGFIGVGLGAISGYIGGKADFLIQKLVEVVWAFPPLLLGITIMAFLGQGLTNLIIA
                     "
BASE COUNT         1438 a         1616 c         1603 g         1369 t
ORIGIN      
        1 tcacggcgag agccggcagc ccgaatcggt atgattcctc gaccagctcg tcgggatggc
       61 tggcgccctc gagaaaagag aaattagttt tgcaccagag cggaacgtag gaagacattt
      121 tgttctattc aaattaaaag ctgccgtaca cgaaggccgc atggcccgtg tcaccttgac
      181 agacaacgga gccattacct agtaagaaga atatttgacc ggcatcctca tggtcaatcg
      241 gtatcttcca ggaccggctt aatcagaaag tcgaacccga gcagcctctg aaattgcgac
      301 caccgccgga tgctttagcc tgcgatccag cgaaattgca taaaaccgct cgcgaatatt
      361 tcccacccgc ccgagggctt tcacatcgta ttctcgttga acttcgacgc tgataatcga
      421 cggtgcaaca aagaggccga gacctcgcgc tccgaacacc ttcaacaggg cgctgtcctc
      481 aaattccgca atgatcatcg gccggatctt ttccgaatcg aaccattgat ccaaggagcg
      541 gcgcaccgtg gtgttatgag tgggcaacaa aaagggtgcg ccatcgaggg accgcgggaa
      601 gccccgccga taggtattca ccaatttctg tgttgcaaac aagattaaac cacactcacc
      661 gagcaagtga ttgaatgcct tcacgttgac agcgggatct atcggggcgt cagtcaggac
      721 gacatcgaga tcatggacag ctaacgacgc cagcaagtca gcgttgctac cctcccggca
      781 aatgattcga taggattctt ttaatcccag ggccggttcg atcaatttat acgcaaccaa
      841 cttgggcaac acatccgcaa ccccgacagt caaacgggcg acacgtcccc cggcacggcc
      901 tttaagagcg ccggtcaact cccgtcccaa agaaaagatc tcttcggcat aacgaaatac
      961 gaccttgccc atatcggtca gagtcagatg cctcccgctc ttgcgaaaaa gcttctcacc
     1021 gatcgctacc tccaaggact tgacttgggc gcttacggtg gcttgggtca agtggagttc
     1081 ctcgccggcg cgggaaacgc tgccgcaacg cgccacgttc cagaaataga aaagatgatg
     1141 ataattgagc cattccacgg ggcgggataa tagttcgatt ttttctatgg atcaagatga
     1201 aattattaat ttgtcgaatg attgcgccgg ccttattgtt tggatggaaa tcgttggcga
     1261 aaggaaagca atgccatgtc agcaattcga aacttgtttt cttcagaaga tagcggacgg
     1321 cttttcgaac ccgatactgt tcttagcgcg caattctacg caacctataa aggcggcccc
     1381 tacaaacagc cggagctccg gttgctggct gcggtgttgg aggatgcgat ctcctgccta
     1441 acagtaaaag cgcgtccagc cacagcgcga cagcgaaaac agtttgacga cgcttggcag
     1501 tggttcgcag cggaagatga tgatagcgaa tggattttct cattcaaaaa catctgtgac
     1561 gctttaggca ttgatccagc atacttgcgc cgggggctga ttcgctgggc ggcggcaaca
     1621 gaactaggct ctgccggcga cctgtcaggc gttagaagcg ccgcctcagc acgcccgacc
     1681 aaaatcaggt tgcgcgccgg ctcatgaccg gcgggattga tcgtgagaaa ttcgactctc
     1741 aacgcgagcg gccgaatgat cgagaaactg gaagactcag caacccggag cgataaattc
     1801 tatcgggggt catcaccaca agcgccactc tattgcatca cacatagccc gctacacgtc
     1861 gacaacatac tgccgtaacc atcgttgaca ttgctgctta agctgcggcg ggaattcatt
     1921 cgcatcgctc gctctaggtc cttccaccgg ggctgtctca agccgaccgc taaaatcctg
     1981 ttacgacggt attgacatcc ggcaaagcag aacgatagaa ggagcctgtc accgccaatg
     2041 actggccaaa ggagaccgct atgtcgccaa agtttcgaaa cctctgcctg ctgatgtcgt
     2101 tgacctcgat cattctcttc acgggcgcgg agcggccgtc ggctcaaacg cgagtgtcta
     2161 tcggcgtcac ggaaacgatt gagactctca atccgtacgg cgacagcgtc tcactcttgt
     2221 acggcatttg gagcgaaatc accggaccat tttgcaccta caactatgaa aaaggagatt
     2281 tcgaaggacg cttggccgag cgttggaagg tagagaaccc gagcacgtgg cttttctatc
     2341 tgaataaaag ctatcgcttc aacgacgggt ccgaagtgac cgccgacgac gttgtccatt
     2401 cgatgatgaa tcgggtgatc aaggatccgc aatccaagca gaaggcgtcg gtggcgggac
     2461 ccattatcaa agcagaggca gtcgataagc acactgtaaa attcaccact gataagccgt
     2521 cggcgccgct gctcgggttt gtttgtgacc gactcatcgt cacgagcaag gcgatgttcg
     2581 acaaatatgg ccgggatgtc gccgacaagg agcatatgct cggcgcaggt ccctaccggc
     2641 tcaaggaact tattcccggc cagcgtatgg tcattgtcaa gcgttccgat cacccggagg
     2701 cgaaaaaaca tccgaacgcg ccggatgaaa tcgtttatcg cgttatgcgc gagaccgaac
     2761 aacgggtgac ggcattgctc aacggcgaaa ttcagatcgc tcaattcatt ccaccgcact
     2821 tacgccatcg tgtcgagaaa tctcccaatt taaagattac cgccacagac tcagtggaga
     2881 tcatgtttct cgccatgcaa cccaagcctc ccttcgataa aaaagaagtg cgccaggccg
     2941 tttgtcacgc gatcaatcgc gatcagatca tcagcacact actggaagga ttcgccagcc
     3001 gtctcgatgg acccatcggg cccgggcagt atggttacga tcctaatttg aagccgaagt
     3061 tgagctacga tccggagcga tcaaagaagc ttttggcgca agcgggctat cctaacggcg
     3121 tcgatgtcga gcttcagacg ccggtgggac gttatacatt ggacaaacag attaccgaag
     3181 cgatgattcc catgttaaac gccgctggat tccgcgccaa gctctcgact cccgagtggc
     3241 cgacactttg ggcgaatgtt cagaaaggaa cggtgccttt ttattatatg ggtcgtggct
     3301 cggttcagga tcccagcgcg gcgctgcacc aatactttca ttcgaaagaa actccgcgaa
     3361 tcggttattc gaaccctaag ctcgatgaac tgttggacaa agaacaggca gagttcgatc
     3421 caaaaaagcg ccgggaatac ctgtcgcagg cgatgagtct catcaccgag gaagcaccgg
     3481 cttgtttcat gtggcgccac aaactcttgt ggggcatgaa taatcgcatt gactacaaac
     3541 cgctgccgga tgcgcggatc ttcggcatgc atatcaaggt ggcaaagaag tagtggtctc
     3601 agcttagtgg ggatcccggg ctataaacgt ctggaccaag tgcagccatg accggggaaa
     3661 aatttatcga tgtagccggc gtaagaacac gctacttcga gaaaggtgac ggaccggtcg
     3721 tcgtattgtt ccacggcggc cactttggtt cgaacgatgc ggccgattgt gctgaagatt
     3781 gggcccttaa ctttgatgga ttggctaagt ggttccacgt ttatgccgtc gacaagatcg
     3841 gccaaggcca taccgataat cctaaaacgg atttggatta tacgatctcg actgtggttc
     3901 agcatgctta cttttttctt aaagctctcg atctgcgcaa cgttcatcct gtagggcact
     3961 cccggggcgc gtatttggta gcccgattga cgttggaaca tcccgagctg tttcgatcgt
     4021 gtattttggc cgacacgaat acgctcgctc cgggcatcag caaaaacgag acggtcatgg
     4081 caaacccgcc gctaccacgg ctcagccgag agagccagag gtgggtgttg caacgttatt
     4141 ccttcgcctt cgagcacatc accgacgaat ggctggacgc gatgatgcgg gttgcggcat
     4201 tggagaaata taaggaagcg gtgaccaaga tggaaaccgc cggactcagg acaacccgct
     4261 ttctacccca tctgtcgagg gagaaggacg aaacgctagg atggattcgg gagcgcggct
     4321 tggccaagcc gaccttgctg gtttggggtt acaacgatcc cacggcgacc ttagatcaag
     4381 gactgggtct gttcgatctg attgcgcgca gtacgccgga ttcccgaatg aagattttca
     4441 atcgggcggg tcattttacc tatcgggagc atccggttga atttaacgaa tcgctgcgat
     4501 cgtttatcca gcgaaacagc gggtaaacgt gaaccgatgg cgagaatttc gtgaaccctt
     4561 gaaagattcg ataaggtgac aagttatctc cttcatcgag tgactggaac gattcctgtt
     4621 ctccttctaa tcagtttgtt agttttcttg ttgattcatg cggcgccggg tgacccaact
     4681 ttgctcctcc taggcgaaga aaccaatgca gcggaagtcg ctagggcgaa ggagcgctgg
     4741 ggattggatc ggccgttgta cgttcaatat cttaagtttc ttaattccgc agttgccgga
     4801 gacttcggca agtctttcaa atacggcgag ccggtgacca gtgtcatcaa atcgagactc
     4861 cctgcgacca tagagctggc ggttttctct atcatcatcg caacttgcct agctattccg
     4921 ctaggagtat gggccggctc ccgacccaat tcgtggctcg acaattttgg aacgaccttc
     4981 ggtcttttcg gtatcagcat gccaagcttt tggcttgcca tcatgctgat cttgctgatg
     5041 gccggagttc tcaatctcct gccgacctcc gggcgcagta cctacggcat cgccggaccc
     5101 gaacaaaccg ggttctacat attggacagt ctgctgcaac aaaactggca ggcggcttgg
     5161 gacgcgctca ggcacatttt attgccggct ctagccttgg gcgtcaatat gctgggaatt
     5221 ttaatgcgtg ttacccggtc ggcaattctt gaggtgatga acgaagagta cgtcactacc
     5281 gcacgcgcta agggattggc tgaaagaagg gtcgtctggc gccacgtcac cagcaacgcc
     5341 ctcataccgg tgattacagt cgtaggcctg gagcttggca ctctgctgag cggctcgatc
     5401 atcgtagaaa ccgtgttcgc atggcccggc agcggcagcc ttcttatcag cgctctcaat
     5461 tcacgcgact atcctctggt caccggactg gtgatgagct ataccgtcgc atttgtcata
     5521 atcaatcttg ttatcgatgg attgtatgcg gtcattgacc ctcgaatccg cttctgaaaa
     5581 gactccgggc gcgatctcga gcgcgttagg acggcttcac gccaaagcat tcagcggcgg
     5641 ttcgatcgtc ttgattttta tccttctcgg aatattcggc cctttgcttg cgccgcacga
     5701 tccaaacaag caagaactca cggccatgat gaagcccccg caagggatcg gtggcgcaca
     5761 cgtactcggc accgacaatc tcgggcggga cattttgagc cgcattgttt atggcgcgcg
     5821 ggtttctttg cttgtggcat ttgccgtggt tttcgtttcc ggctttatcg gtgtcggcct
     5881 cggcgcaata tccggctaca tcggcggcaa agccgatttc ctcattcaaa aattagtcga
     5941 ggtcgtttgg gcgtttccgc cgcttttgct cggtattacc atcatggcct ttctgggcca
     6001 gggtctaacc aacctgatca tcgcct
//
LOCUS       DASUTG010000732         3730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1297316, whole genome shotgun sequence.
ACCESSION   DASUTG010000732 DASUTG010000000
VERSION     DASUTG010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297316"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF089_16935"
     CDS             <1..208
                     /locus_tag="VF089_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175698.1"
                     /translation="AFLLLGAVFSGFYFLSPSKSPERSNPSAATEPVSRTSDEDASGI
                     GELKQPAKASETKTPHSKSPSLKK"
     gene            229..555
                     /locus_tag="VF089_16940"
     CDS             229..555
                     /locus_tag="VF089_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175699.1"
                     /translation="MKNELQNLTEDISRLYEEITQKTESLGQIDSITRLYDELQTQLL
                     GISTEEVDLLQTQIKSTLEQMVGISKSLGVIKTLKITLNSNDEVLESVKKTQTNTTGR
                     RSLEDR"
     gene            583..1377
                     /locus_tag="VF089_16945"
     CDS             583..1377
                     /locus_tag="VF089_16945"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX6175700.1"
                     /translation="MQGNLSQISLNDILMLATGGKKSGVLKLSRGKETVEVYLLDGSI
                     IHATCPIGDGEKALLYPVTWGEGNFALLPTGTPPSTTIKKSSSEILEEVKAMSREWEN
                     ILEVIPSGKTILRIADLGEEQNGPVTVPHVGWRVLSKIDGNRSVQEIAEALRIPYAYT
                     AKVIFNLQKAGLVEIIAPSSRPTTDLVPATVLARVVELLTDIIGPMASLVVRDQIEAL
                     GETQAQFPESKLHDLIERVAKEIGDVKLRQKFEDSAYQEISTVKRF"
     gene            1405..2187
                     /locus_tag="VF089_16950"
     CDS             1405..2187
                     /locus_tag="VF089_16950"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX6175701.1"
                     /translation="MSQESISIETTKVNKPLNITWQGGSLRWKISVSFSGLILLLGLL
                     VIGIVYFMTNNALHRQVDLRAMAIATNLSDASAAYVSRRNTLELDVLIAKYGRLDGVA
                     YAYIQDPKGEIVATSLQPFPAELRDSTVVERRTASSRVTNIRGKSIYDTRVPLLEGQL
                     GTVHVGLWADSVQADVRGTVLTIITLIAGCLVLGLVLSLLLAAKAIRPILELKASADE
                     ISRGQLDTPVTIQSTDEIGELARSLERMRASLKAAMVRLNRE"
     gene            2223..3647
                     /locus_tag="VF089_16955"
     CDS             2223..3647
                     /locus_tag="VF089_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175702.1"
                     /translation="MLQFMKQIFTRVLTVTALSALPFGAVVSDSKSAESTANQPLRKV
                     MVGYSAISPSQAPAWIAQDQGFFRKYGLDSQLLFVEGGSRTVQTLISGDVLAAQVAGS
                     SVLQSNLQGSGVVMVAGFLNTMDYKLIVSRDITRPDQLKGKIMAVSRIGSSSDFATRY
                     ALEKYGLAPDKDVAIVQIGSQPARFSALESGKIHGVMIAIPLTAKAKRMGLNTLADLQ
                     MLGLEYQHTGLAFSQDTLKKQPELVRNVLKAFVEAIHFMKTRRQETIGILAKYLKSDD
                     TEALDEAYDSVLNSLIPEKPYPTIKGIQIMLREMGVKEPAARSARPEQFVDLTFMKEL
                     DSSGFVDHLYKTQRLAKAAPRANPAPVVMATKEKSETVETKIKPAAVESKEIEIVKAA
                     TPAPEKTQPPVASIAKPVKTGNPVDQQYVVKVGDTLSKLAERFYNSTSKWEKIFEANR
                     ENLKNPNYIYVGMKLIIPADDQAS"
BASE COUNT         1041 a          912 c          941 g          836 t
ORIGIN      
        1 tgcgttctta ttgctaggtg cagttttttc cggcttctat ttcctgtcac catcgaaatc
       61 accagagaga tcgaatccat ctgcggcgac cgaacccgtc tcaaggacat ccgatgaaga
      121 cgcttccgga atcggggagc taaaacaacc ggctaaagcg agtgaaacaa agaccccgca
      181 cagtaagtct ccgagtttaa aaaaatgaag aaagaaggct gaacaattat gaagaacgaa
      241 ctgcaaaatc tcactgagga tatctcccgc ctatatgaag aaatcactca gaaaaccgaa
      301 tcgctgggac agatcgatag catcactcga ctctatgatg agctacaaac tcagttacta
      361 ggcatatcga ccgaggaggt cgacctcctc caaacacaga tcaagtcgac gttggaacag
      421 atggtcggaa tcagcaagag ccttggcgtc atcaagacgc tgaagattac gctgaacagc
      481 aatgatgaag ttctggagag tgtaaagaag acgcagacca atactaccgg gcggcgttcg
      541 ttggaagatc gctagacctt cagccttata ccgggaatag agatgcaagg aaacctctca
      601 cagatcagtc tcaatgacat tttgatgctc gctaccggcg gcaagaaatc cggggttctc
      661 aaactctctc gaggtaagga aacggtagaa gtttatctcc tcgatggcag cattattcac
      721 gccacatgcc caatcggcga cggcgaaaag gcgcttctct atccggtaac gtggggagaa
      781 ggtaatttcg ctttactgcc tacaggaact ccaccatcga cgaccataaa gaagtcctcc
      841 tcagaaattc tcgaagaagt aaaggcgatg agtcgcgaat gggagaatat cctcgaagtt
      901 attccgtccg ggaaaaccat ccttcgtata gctgatttgg gtgaagaaca aaacggtccg
      961 gtaactgttc cacacgttgg ctggcgagtg ctgagcaaaa tcgacggtaa ccgctcagtg
     1021 caggaaatag ccgaagctct tcgaatcccg tatgcgtata cggccaaggt tattttcaac
     1081 ctacaaaagg ccggtctggt tgaaatcatt gcgccatcct cccggccgac aactgatctg
     1141 gtcccggcaa cggtattggc gcgcgtggta gaactcctca ctgatattat cggtccaatg
     1201 gcgtctctcg tcgtacgcga tcaaatcgaa gcacttggtg aaacgcaagc ccaatttccg
     1261 gaatccaagc tccacgacct aattgaacgt gtcgctaagg agatcgggga cgtaaagctc
     1321 cggcagaagt ttgaagactc tgcctatcag gagatctcca ctgtcaaacg attctagtaa
     1381 tcctgcagtc gatccggagg aatgatgtca caagaatcca tctcaataga gacgacgaag
     1441 gtcaataagc cactgaacat aacatggcag ggaggcagcc tcaggtggaa aattagcgtt
     1501 tcttttagcg gcttgattct actgctggga ctactggtta ttggaattgt ctatttcatg
     1561 accaacaatg ccctgcaccg acaagttgac cttcgcgcga tggcaatcgc aactaattta
     1621 agtgatgcgt cagcggctta tgtatctcgt cgaaacaccc ttgaattgga tgttttgatt
     1681 gccaaatacg gtcgtctcga cggtgtggcg tacgcctata tccaagaccc taaaggagag
     1741 atcgtcgcga caagccttca gccgttcccc gccgagctga gggattcaac cgtcgtggaa
     1801 cgtcgcacgg ccagttcgcg tgtgacgaac attcgcggaa aatcgattta cgacactcga
     1861 gttccgcttc ttgaaggaca gctcggcacc gttcacgtag gcctatgggc ggattcagtc
     1921 caggcagacg ttcgtggcac ggtattgact atcatcactt tgatcgccgg gtgccttgtg
     1981 ctcggtctgg ttctatcact actgctcgcc gcaaaagcga ttcgacctat attggagttg
     2041 aaggccagcg cggatgagat cagccgggga cagctggaca cgcccgtgac aattcaatcg
     2101 acggatgaaa ttggagagct tgcgcgatca cttgagcgca tgcgagcaag cctaaaagcg
     2161 gcgatggtcc gattgaatcg cgaatagatc tcgaaggact caataatatg ggaggatcga
     2221 aaatgctgca atttatgaag cagatcttta caagagtgct tacagtcact gcgttgtctg
     2281 cgctaccgtt cggtgccgtg gtgagcgatt cgaaatctgc agagagtacc gcaaatcaac
     2341 cgctgagaaa ggtaatggtc ggctatagcg cgatttcacc aagtcaagcg ccggcttgga
     2401 tcgcgcagga ccagggattt ttccgaaagt acggcttgga ctcacagctt ctttttgttg
     2461 agggagggag tcggaccgtt cagacgctta tatcaggtga tgtcctggcc gctcaggtag
     2521 cagggtcatc tgtgcttcaa agtaacttgc aaggatccgg agtcgtaatg gttgccggat
     2581 ttctcaatac gatggattat aagttgatcg tgtcccgtga cattacccga ccggatcaac
     2641 tcaaaggcaa aataatggct gtcagtcgca ttggctcttc ttcagacttt gcaactcgct
     2701 atgcgctaga gaaatacggg ctggcgccgg ataaggatgt cgctattgtt cagatcggta
     2761 gccaacctgc ccgattctca gccttagaaa gcggcaaaat acatggcgtc atgatcgcga
     2821 tccccttaac cgctaaagca aaaaggatgg ggctcaatac tcttgcggac ctacaaatgc
     2881 tcggacttga ataccagcat accgggttag cctttagtca agataccctc aaaaaacaac
     2941 cggagctcgt tcgaaacgtc ttaaaagctt ttgtcgaggc tattcacttc atgaagacac
     3001 gccgtcaaga gacaatagga attttggcga agtatctgaa atcagacgat acagaagctt
     3061 tagatgaagc ctacgattct gtccttaatt cgctgattcc agaaaagccc tacccgacaa
     3121 ttaaaggaat tcaaatcatg ctccgtgaaa tgggtgtcaa agaaccagca gctcgaagtg
     3181 cgcgccctga acagttcgta gatctgacgt tcatgaaaga actcgacagc tctgggttcg
     3241 ttgaccacct atacaagaca caaaggctgg ctaaagccgc gccgcgggct aatcctgcgc
     3301 ctgtggtgat ggccaccaaa gagaagtctg aaactgttga gaccaagata aagccggccg
     3361 ccgtagagag taaagagatt gagatcgtta aagctgccac accagcccca gaaaaaaccc
     3421 aacctccagt agctagtatc gccaagccgg tcaaaaccgg taacccggta gatcagcaat
     3481 acgtcgtcaa ggtcggcgat accctcagca aattggccga gcgcttctac aattctacgt
     3541 ctaaatggga gaagattttt gaagctaata gagagaatct aaagaatcca aactacatct
     3601 acgtgggtat gaagctgata attccggctg acgatcaagc gagctgattc agcagaagag
     3661 ccatcgaaaa aggggctgaa cggttcatcc gttcggcccc ttttgattac tcgtggagct
     3721 atgaagctag
//
LOCUS       DASUTG010000733         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_706312, whole genome shotgun sequence.
ACCESSION   DASUTG010000733 DASUTG010000000
VERSION     DASUTG010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706312"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VF089_16960"
     CDS             complement(<1..553)
                     /locus_tag="VF089_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175703.1"
                     /translation="MIRCLDVRKNYRQGDNEVTVLSGITLDIPQGQFATIMGPSGSGK
                     STLLHLMGGLDRPSAGEVLVNERIISQMADDQVTLFRRTKIGFVFQFFNLLPTLTALE
                     NITLPFVLDGRPKGKADQRAQFLLDKVGLAHRRNHLPEAMSGGEIQRLAIGRALAFEP
                     PIILADEPTGNLDSKNGAIVLNLL"
     gene            complement(566..937)
                     /locus_tag="VF089_16965"
     CDS             complement(566..937)
                     /locus_tag="VF089_16965"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6175704.1"
                     /translation="MEAQDDPRLQKGIDEFNQQLFFECHETLEEIWLEEHGEDRKFYQ
                     GIIQIAAGYFKLQQGVPAGAIKLWRMGLEKIEPYSPVYLGVELASFSAAVKQNLHELE
                     AVQKTGTDSPALAAPTLCRVS"
     gene            1042..>2201
                     /gene="rlmD"
                     /locus_tag="VF089_16970"
     CDS             1042..>2201
                     /gene="rlmD"
                     /locus_tag="VF089_16970"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX6175705.1"
                     /translation="MIQTNAKNELTLDIDTLSYGPYGIGRHQGKAVMIAKAAPGDTVA
                     ARIVEAKERYSIGEIVRVLKRSSQRQEPPCPYVAQCGGCPWQHVRYESQLVAKQKNIE
                     DALRRIGKLEAFELKPIIPSPITYQYRRRIRLQRDGSKRLGFFTSFSHHLIEIDRCLI
                     ADDKLNGLIEPLRRWSEELITEVEHLEFVTGDQPAQIVVIGQSAGAFNSRDSSVCERL
                     LKENHLINGLIVCGRDWRRTWGEVAVTVIPEDGMCVRVDGDVFTQVNSQGNRQLLKEI
                     LAAADFNQGDRVLELYSGAGNFTHSIAKRVGDIVAVEGYGPAVESGKRTAESNRINNI
                     HWICSPVPAALGQLKKQRQRYSKIVLDPPRTGAKGLERELAAVGAEKILYIS"
BASE COUNT          511 a          608 c          616 g          466 t
ORIGIN      
        1 gcaacaggtt cagaacgatc gcaccatttt tggagtccag gttgccggtg ggttcgtcgg
       61 ccaagataat cggcggctcg aaggctaaag cccgaccgat cgccaatcgt tggatttctc
      121 caccggacat tgcttcaggc aaatggtttc ggcgatgcgc caagccgacc ttgtcgagaa
      181 gaaactgcgc tcgttgatcg gctttgcctt taggacggcc atcgagcacg aaaggtagag
      241 tgatgttctc cagcgccgtc agagtcggca ggagattgaa aaattgaaaa acaaagccga
      301 tcttggttcg gcggaaaagt gtcacttgat catccgccat ttgcgatatg atccgctcat
      361 ttaccaggac ctctccagcg ctgggacgat ccaacccgcc catgagatgc agtagcgtgc
      421 tcttgcccga accgctgggt cccataatcg tcgcgaactg accttgcgga atatcgaggg
      481 taattccgga taggacggta acttcgttat cgccctggcg gtagtttttg cgcacgtcga
      541 ggcagcgaat catgcggtgc gatgattaac tgacccggca gagggtagga gcagcgagag
      601 ccggagaatc cgtccccgtt ttctgaacgg cttccagctc atggaggttt tgtttgaccg
      661 ccgcgctgaa ggaggccagc tcaaccccga gataaaccgg gctgtagggc tcgatctttt
      721 ccagtcccat gcgccaaagc ttgatcgctc cggccggcac gccttgctgt aacttaaagt
      781 aacctgcggc aatttgaatg atgccctgat aaaacttccg gtcttcgccg tgctcttcca
      841 accaaatctc ctccagggtt tcgtgacact caaagaagag ctgttgattg aattcgtcaa
      901 ttcccttttg caatcgcgga tcgtcttgcg cttccatcag atctgtccct cgcgcgcgac
      961 tcagcagccc cgagagtggc aacctcctcc acagcttgta tagtctatag aggatccaag
     1021 attcagttta agattgcgcg aatgatacag actaacgcga aaaacgagct cacgctagat
     1081 atcgatacgc tttcctatgg cccctatgga atcggccgac accagggaaa agcggtgatg
     1141 attgccaagg cggcgcccgg cgataccgtc gcagcgcgta ttgtcgaagc caaggagcga
     1201 tattcaatcg gcgaaatagt ccgtgtgttg aagcggtcat ctcagcgtca ggaaccaccg
     1261 tgtccgtatg tagctcagtg cggcggctgc ccctggcagc acgtgcgcta cgaaagccag
     1321 ctggtggcga agcaaaaaaa tattgaggac gctctgcggc gcatcggcaa actcgaagcc
     1381 ttcgaactca agccgatcat cccttcgccg attacctacc agtatcgacg acgtatccgt
     1441 ttgcaacggg atggatccaa acggctcggc ttctttactt cattttcgca ccacctgatc
     1501 gagatcgaca ggtgcctgat agccgatgac aagctcaatg gtctcatcga accattgagg
     1561 cggtggagcg aggagttgat cacagaggtc gagcatctgg agtttgtcac gggcgaccaa
     1621 ccggcacaaa tcgtcgtcat cggacagtca gcaggcgcat ttaactcgcg cgacagctcg
     1681 gtctgcgaac gcctgctgaa agaaaatcat ctgatcaatg gcttaattgt gtgcgggcgt
     1741 gactggcgcc ggacctgggg agaagtggcg gttacggtca ttccggaaga cggcatgtgt
     1801 gtgcgagtcg acggtgacgt atttacccag gtcaacagcc agggcaatcg acaactcctc
     1861 aaagaaatat tggcagcggc agatttcaac caaggagacc gcgtgctcga gctatacagc
     1921 ggcgccggaa atttcaccca ctcgattgcc aagcgggtcg gtgacatcgt cgcggtggaa
     1981 ggctatggtc cagccgtcga gagcggcaag cgcactgccg aatcgaaccg catcaataat
     2041 attcactgga tttgctcgcc ggttcccgcg gcgctggggc aactaaagaa gcagcgccag
     2101 cgctactcta aaatcgttct cgacccgccg cgcaccggcg ccaaaggtct cgagcgcgag
     2161 ctggcggcgg tcggtgccga aaagatcctc tacatctcgt g
//
LOCUS       DASUTG010000734         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1447338, whole genome shotgun sequence.
ACCESSION   DASUTG010000734 DASUTG010000000
VERSION     DASUTG010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447338"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            491..886
                     /locus_tag="VF089_16975"
     CDS             491..886
                     /locus_tag="VF089_16975"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6175706.1"
                     /translation="MFVCSGNSCRSQMAEGFARAAGVEAYSAGTEPAGYVHPVAVQVM
                     AEKGIDISGQTSKELDPKLAETMDAVITVCGEADETCPAIPHVNRLHWPIPDPAKATG
                     SQEEVVGVFRAVRDDIARQVSDLLEQTKG"
     gene            962..1645
                     /locus_tag="VF089_16980"
     CDS             962..1645
                     /locus_tag="VF089_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HEX6175707.1"
                     /translation="MINEYAPVGASDNSPDWFYPEWAANAPNNQPIVVRDTDSALGRY
                     NLQTKEIGLKDIARIHGHMCDGLVIAFVEIKAVLSKVFPDGVVDRTDLQVVSKNGPCW
                     ADAAAFLTGARINFKTLRIDASIAGGFIIQRISTGEAYHVHLKRGVFPDDQVVLENRI
                     RTLRAQGNPVTAEYIDTVEKMGDSLSLKLLTVPPENLLEIKRLNDYKFNFTDMFGDRG
                     DIINKNMPR"
     gene            1724..2836
                     /gene="arsB"
                     /locus_tag="VF089_16985"
     CDS             1724..2836
                     /gene="arsB"
                     /locus_tag="VF089_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX6175708.1"
                     /translation="MAQERLTKKLSFLDRYLTLWIFLGMFVGVGWGYLFPGVVGFWNQ
                     FQSGTTNIPIALGLILMMYPPLAKVKYEELGEVFRNYKVLALSLVQNWIIGPVLMFIL
                     AVVFLRGYPEYMVGLIMIGLARCIAMVIVWNDLAKGDTEYCAGLVAFNSIFQVLFFSV
                     YAFIFITVLPPLLGLTGVEVNITIGQIAESVFIYLGIPFIAGVITRFTLIKLKDKQWY
                     HEKFIPKISPITLIALLFTIVVMFSLKGEFIVKIPLDVVRIAIPLLIYFVIMFLLSFY
                     MSKKVGATYAQSTTLSFTAASNNFELAIAVTVAVFGINSGQAFAAVIGPLVEVPVLIG
                     LVNVALKFRQKYFGVTGEPMTSTRLSPVQKTSAVRK"
     gene            2951..>3172
                     /locus_tag="VF089_16990"
     CDS             2951..>3172
                     /locus_tag="VF089_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175709.1"
                     /translation="MKTISEEAGRFDPRSAEGLNRGCVCRPLDPDRLRRELEAEPSLA
                     GLDENIARTRPHLFSATAAFMSREHFSGIQ"
BASE COUNT          738 a          774 c          876 g          784 t
ORIGIN      
        1 ttacgtaatt acagttcccc aacaacgcga aattcttgtc catcattcgc agatgcgcga
       61 gaaccccgtt cagtccagcg gaagaattca gcgtatgaca ggccttatag gaatgggccg
      121 cgaaggcttc aggcatattt cttgccccgt agagtaactg taattcgtca agacctgagt
      181 ttggatgttc tatgattaac aacgagattc ttccacgtga ggaggcgacg attaaaaaaa
      241 taagtgatcg acatttttcc ccacggcgtg gagctagaag tctacgtgta cggtccggtg
      301 gagagagccc tggatgatcc ggcgcaccgg aatatcgcaa gactctgctt agtctcggca
      361 agccggagtg taggtgcatc tgtgccgcga caaagcggca atctttatgc taggtcccag
      421 agcgcaatcc gtatcgttga actcggcaga gcgtcgaagc caaaaggaag gaagtgacga
      481 gttgaaggtt atgtttgtat gctccggcaa ctcctgcagg agccagatgg cggaagggtt
      541 tgcgcgagcg gccggggtcg aggcttacag cgcgggcacc gaaccggccg gctacgtgca
      601 cccggttgcg gtacaggtaa tggcggagaa gggaatcgac atctccggac agacttctaa
      661 agagttggat ccgaagctcg ccgagaccat ggacgcggtg attaccgtgt gcggcgaggc
      721 ggatgaaacc tgtccggcga ttccgcacgt caatcgcctt cactggccca ttccggaccc
      781 ggccaaagca accgggtcac aagaggaggt cgtcggtgtt tttcgcgccg tgcgcgacga
      841 catcgcacgg caggtgagcg atctcctaga acaaactaaa gggtagcggt gtggggcgac
      901 gcaattcagg aatacagaaa atattttctc gcttcccgga acttgggatg aggaggttgc
      961 aatgatcaac gagtatgcac cagtcggcgc aagtgataac agcccggatt ggttctatcc
     1021 ggagtgggca gcaaatgctc ctaataatca gcccattgtg gttagggaca cggatagtgc
     1081 cctgggtcgt tataacctgc aaacgaaaga gattggttta aaagatattg cgcgtattca
     1141 tggccacatg tgtgacggtc tggtgattgc gtttgtcgag atcaaagctg tattgagcaa
     1201 agtgtttccc gatggcgtgg tcgaccgaac cgatctccag gttgtttcaa aaaatgggcc
     1261 ttgttgggcc gatgcggcgg cgtttttaac cggcgccagg atcaatttca aaactttgag
     1321 gattgatgcg tccatcgctg gtggattcat cattcagaga atttcaaccg gcgaggctta
     1381 ccatgtgcat ttaaaacgcg gcgtttttcc ggatgaccag gtcgttcttg aaaacagaat
     1441 tcggacctta cgggcgcagg gaaacccggt aactgcggag tatatagata cggttgaaaa
     1501 gatgggggat agcctgagct tgaaactcct gaccgttccg cccgaaaatc tattggagat
     1561 aaagagattg aacgattata aattcaattt tacagatatg tttggcgatc gcggagatat
     1621 cattaacaag aatatgccga gatgaataga atctgcttgc gaaaggaagc gctctatttc
     1681 gaatcgcaga aaaatacaag gatcagcaaa taggagcagg acgatggctc aagaacggct
     1741 cactaaaaaa ctttcttttc ttgatcgcta tctgacgtta tggatctttc tcggaatgtt
     1801 cgtcggcgtg ggatggggct atctttttcc aggtgtcgtc ggcttctgga atcagtttca
     1861 atccgggacg accaacatcc cgattgccct cgggctgatc ctcatgatgt atccaccctt
     1921 ggccaaggtg aaatacgaag agctcggcga ggtattccga aactacaaag tgctcgcgct
     1981 ctccttggtt cagaactgga tcattggccc cgtcctcatg ttcattctgg cagtcgtttt
     2041 tctgcgggga tatccggaat atatggtggg gctgatcatg attgggctgg ctcgctgtat
     2101 cgccatggtc attgtctgga atgacttggc caagggagac accgaatact gcgccggctt
     2161 ggtggccttt aactcgatct ttcaagtcct tttcttttct gtctacgcgt tcatctttat
     2221 cactgttcta ccgccccttc ttgggctcac gggggtggaa gtgaacatca cgattggcca
     2281 aatcgccgaa agtgttttta tctatctggg tattcccttc atcgccgggg ttatcacccg
     2341 atttactctg atcaagttga aagacaagca gtggtaccac gaaaaattca ttcccaaaat
     2401 tagtcctatc acgctgatcg ccctgctatt caccatcgtg gttatgttct cgctcaaggg
     2461 tgagttcatc gtcaagattc cattggatgt tgtacgcatt gctatcccct tgctcattta
     2521 cttcgtgatc atgtttctgc tgtcgtttta tatgagtaag aaagtggggg ccacctatgc
     2581 tcagagtacc accctttcat tcaccgctgc ctcgaataat ttcgagcttg ccattgccgt
     2641 gactgtggcg gtcttcggga tcaattccgg ccaggccttt gcggctgtca tcggaccatt
     2701 ggtggaggtt ccagtgctca ttggccttgt caatgtcgcc ttgaagttcc ggcagaagta
     2761 cttcggcgtg acaggggaac ccatgacgag tacgaggctg agcccggtac aaaaaacatc
     2821 ggctgttcgc aagtagcggg gagcagaagc cctaccccgt tgttccttcg ttgctcttaa
     2881 agatgaggag ccgcacccag cagttgctgt actacctttg cgacgaggcc gtcgagttgc
     2941 accagatgtt atgaaaacca tatcggaaga ggcgggcagg tttgatccgc gttccgcaga
     3001 gggcctgaat cgcggctgtg tttgtcgccc actggacccc gatcggctgc gccgagagct
     3061 ggaagcggaa ccctccctcg ccggcctgga cgagaacatt gcgcgcacgc ggccgcatct
     3121 gttctctgcc acggccgctt tcatgtcccg ggagcacttc agtggcatac ag
//
LOCUS       DASUTG010000735         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_113971, whole genome shotgun sequence.
ACCESSION   DASUTG010000735 DASUTG010000000
VERSION     DASUTG010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113971"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VF089_16995"
     CDS             <1..761
                     /locus_tag="VF089_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6175710.1"
                     /translation="FNRPEKRNAMSPQLHREMFDLLTEIRYDKETRVVVLTGAGDNFC
                     AGQDLKQYSLEMESQPERVRDEVREKVRRWRNEMLRTLPQPVIARITGWCLGGALTVV
                     AGCDIALAAEDALFGLPEVNFGHFPAGDTTSVMTEHLKPKHGLYYALTGKMMTAREAE
                     RIGLISKAVPKSELDREVAELARSLREKSPLGLKAVKEAWYYSSYAAPDVAYEISNLI
                     SQRTIRDHGGRPGLEQFVQKKLRPTSGVMQLSDK"
     gene            886..1932
                     /locus_tag="VF089_17000"
     CDS             886..1932
                     /locus_tag="VF089_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175711.1"
                     /translation="MKFSIMVITLILALAANGWAQGSKPNTLAELAKYNAPDRERILY
                     EGAKKEGKLLWYTSLVPNKHIAKVFQAKYPGVSVDVYRAAGNTLATRLLEERRAKRYL
                     ADAIESTPPQLMLLRDAQALQPFLSPHLANYPDVAKESASGDLVFWATDRESLIGVGY
                     NTNVIPAALVPKKFDDLLNPALKGKMSTANNETGARAIGAILKAKGEAFVRKLQAQDI
                     RPHSTTAAGLTDLIISGEAPLSFTMVQTNLTQPAATKGAPVAWVPMEVVAVNAGSAAV
                     IANAPNPHAALLFVDFLLGPDGQKMYSEELFYGSAAKVSGFERWYPEKGLTTEEYEKR
                     AEGWLQLLQEITRN"
     gene            complement(2316..3791)
                     /locus_tag="VF089_17005"
     CDS             complement(2316..3791)
                     /locus_tag="VF089_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175712.1"
                     /translation="MKHTIDPDGQRLPIKLDSTSNGEFTPVPLSPTNRAANRLAHQAA
                     SQNAKRLGIGRRDFLISACGAASTLLAFNAAAAAVGKRGGFFELEAEAALEPQMAQAR
                     VGSKGEFIFDVQGHFVDPTGAWVKTAPADAFKWSPKASCGLAAKPGTRSYLNCLGPEE
                     FVKDVFLDSDTDMMVLSFVPSRRDSEPLTIQAADAVRRTIDRMEGSHRLLLHGRVNPN
                     QPGDLEAMEELKERWKVSAWKTYTQWGVDGKGFFLSDDIGTRFIEKARALGVKVICVH
                     KGLPFGKQSYEHSQCSDIGVVARRFPDVSFLIYHSGFVTGVPERAFTGRGAGDGIDTL
                     IRSLIENEVKPGSNVYAELGSTWRFLMRDPDGAAHALGKLVKYCGENNVLWGTDSIWY
                     GSPQDQIEAFRTFQIAPELRAKHGYPEITPALRAKIFGLNASRIYAISADEVKKHTRA
                     DRIGKERLAYREHPEPHYLTYGPKTRREFLNLLGWNGGSRA"
     gene            complement(3949..>5433)
                     /locus_tag="VF089_17010"
     CDS             complement(3949..>5433)
                     /locus_tag="VF089_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175713.1"
                     /translation="CGIPVIAEDATSAEETLQRIASNGYDCVLLDYYIPGVRGLALFQ
                     KIREVAANMPVVIFTGRGDEDIAVELMKAGAADYLPKASLTAERLASSLRHIIELSGA
                     AEARRRAEEELRAQEARFRTLVNAIPQLAWMADRTGAAYWFNDRWYDYTGTSFEEAQG
                     WGWRKAHHPDHLQRVVESLRRSFEAGDDWEETHPLRGKDGTYRWFLSRALPIRGEDGS
                     ITNWLGTNTDITDQKNAEAERERALALEQQLRSQAERATRARDEVMAIVVHDLRNPMN
                     TILTSATIIGYSLDKQADKAIDISNPALSADIKTCVEAIQSSVKTMDRLICDLLDVAR
                     MEAGSFSIQPSHVDIGALLEESLKLCESQAWAKKITVTADIPTGLPAVNGDRDRLDQV
                     LSNLLGNAFKFTPEGGRVMVRARKVDDSVQITVEDSGPGIPAADLPRIFDRYWRGDRA
                     SRDGAGLGLAICKGIVDAHGGNIWVESTVGRGTTFRFTVPCATE"
BASE COUNT         1168 a         1604 c         1560 g         1101 t
ORIGIN      
        1 catttaaccg tccggagaag cgcaatgcga tgagtcctca attgcatcgt gaaatgttcg
       61 atctgttgac cgagattcgc tacgacaaag agacccgtgt ggttgtgctc accggggccg
      121 gtgacaattt ttgcgccggg caagacttga agcagtattc tctcgagatg gaaagccagc
      181 cggagcgggt gcgcgacgaa gtccgggaaa aagttcgccg ctggcgcaac gagatgctgc
      241 gcaccctgcc ccagccggtc attgcgcgca tcaccggctg gtgtctgggc ggagcgctca
      301 ccgtggtggc cggctgcgac atcgcgctgg ccgctgaaga tgcgctcttc ggtttgcctg
      361 aagtcaattt cggccatttc cccgccggcg ataccacctc ggtgatgacc gagcatctta
      421 agcccaagca cgggctctac tacgcgctca cgggcaagat gatgaccgcc cgagaggccg
      481 aacggattgg cctcatctca aaggccgttc cgaagtcgga attggatcgg gaagtcgccg
      541 agctcgctag atccctcaga gaaaagagtc cgctgggctt aaaagcggtc aaggaggcgt
      601 ggtactacag ttcctatgca gcaccggacg tcgcctacga aatctccaac ttgatttcgc
      661 agcgaaccat acgagatcac ggcggccggc caggcctgga gcagtttgtc cagaaaaaac
      721 tcagaccgac atcgggcgtg atgcagctaa gcgataaata atgatcggca agcatttcac
      781 ttggagatgc aatcagcggc agcgaacagg aacacatgtc tcagctcgac cttgtcgatc
      841 atcggctggc cgtgttacca aagcagatag aggagcaaaa gagtaatgaa attttctatc
      901 atggtcataa ctttgatctt agccctggcg gcaaacggat gggcgcaggg cagcaagccg
      961 aacacgctcg ccgagttagc gaaatacaac gcaccggacc gcgagcggat tctttacgaa
     1021 ggcgccaaga aagagggcaa attactctgg tatacatccc tggtgccgaa caaacacatt
     1081 gccaaagtgt ttcaagctaa ataccccggg gtgtcagtcg acgtttaccg cgccgccggc
     1141 aatacgctcg ctactcgact gttggaagaa cgccgcgcca aacgttacct ggcggatgcg
     1201 atcgaatcga ctccgccgca gctcatgctg ttgcgcgatg ctcaggctct acaaccgttt
     1261 ctgtcgccgc acctggcgaa ctatccggac gttgccaagg aatcagcgtc gggcgatctg
     1321 gttttctggg cgaccgaccg ggaatctctc atcggcgtgg gctacaacac caacgttata
     1381 ccagcagcgc tggtgccgaa gaaattcgac gacctgctga atccggcgct caaaggcaag
     1441 atgtcaacgg caaacaacga aaccggcgcc cgggcgattg gcgcgatact caaggccaag
     1501 ggcgaagcct tcgtcagaaa gttacaggca caggacatcc ggccgcattc gaccacggcg
     1561 gccggactga ccgacctgat catctccggc gaggcgccgc tgtcgttcac catggtgcaa
     1621 accaatctca cacagcccgc cgcaaccaaa ggagcgccgg tcgcctgggt gccgatggaa
     1681 gtagtggccg tgaacgcagg cagcgccgcc gtcatcgcca acgcgcccaa tcctcatgcg
     1741 gctttgttat ttgttgattt cttgctcggg cccgatggac agaagatgta ctcggaggaa
     1801 ctcttctacg gcagcgcggc gaaggtatcg ggattcgagc gctggtaccc ggaaaaagga
     1861 ctgaccacgg aggaatacga aaagcgcgcc gagggttggc tgcagctgct gcaggagatc
     1921 acgcgtaact gacgcaatgt gtgaaataga aatgcagagc tgtcccttct atttcgccga
     1981 gcacgttgtt gaaacgctgg ttacagactg ctcaaagaag atcgagggac ggcgttgagc
     2041 acagattccg cagtagagat agatcattgt gtagtaatcg aggggcgagc ctggaggagt
     2101 gaaggcacaa aagcaatttt ttcaatgccg tttatggtgc ttggccctga gccgacaact
     2161 ttttcacgcg gccgctaaaa cactggagat tgcaggccgc tatcaaccag acagctattg
     2221 ccttcactcc ggagggttcg tccctttgat cgctgtgtta ctttgaactg cgtaatttgg
     2281 ggtgcaacgc agtatccggg tcctttcacc agctgctaag cgcgcgagcc gccgttccaa
     2341 ccgagcaggt ttaaaaactc gcgccgggtc ttgggcccat acgtcagata atgcggctcg
     2401 ggatgctcgc ggtacgccag gcgttccttg ccgattcgat cggcgcgggt gtgcttcttc
     2461 acttcatccg cgctgattgc ataaatccgg ctcgcgttca gcccgaaaat cttggcgcgc
     2521 aacgccggtg tgatctcggg gtagccgtgc ttggcgcgca gttccggcgc aatctgaaaa
     2581 gtgcgaaacg cctcaatttg atcctggggc gagccgtacc agatcgaatc cgtgccccac
     2641 aaaacattgt tctcgccgca atatttgacc aacttgccca gcgcatgcgc cgcgccatcc
     2701 ggatcgcgca tgaggaagcg ccaggtgctg ccgagctcgg cgtaaacgtt gctgccgggt
     2761 ttgacttcat tttcgatcag cgagcggatg agtgtgtcga tgccgtcgcc ggcgccgcga
     2821 ccggtgaagg cccgctcggg aactccggtg acaaaacccg agtggtaaat gagaaaactt
     2881 acatccggaa aacgcctggc gaccacgccg atgtcgctgc actggctgtg ctcgtaggat
     2941 tgcttgccga acggcagccc cttgtgtacg cagatcacct tcacgccgag cgcgcgcgcc
     3001 ttctcgatga agcgggtgcc gatgtcatcg gataggaaga atcctttgcc gtccacaccc
     3061 cactgggtgt aggtcttcca cgcgctgact ttccagcgct ctttaagttc ctccatcgcc
     3121 tcaagatcgc cgggttgatt cggattgacc cgtccgtgaa gcaacagccg gtgcgagcct
     3181 tccatccggt caatcgtgcg gcgcaccgca tcggcggcct gaatcgtcag cggctccgag
     3241 tcgcggcggg acggcacgaa cgacagcacc atcatgtcgg tatcgctgtc gagaaacacg
     3301 tctttaacaa actcctcggg tccgaggcag ttgagataac tgcgcgtgcc aggcttggca
     3361 gccaggccac agctcgcttt gggcgaccac ttgaaagcat ccgcaggagc tgtcttcacc
     3421 cacgccccgg ttggatcaac gaagtggccc tgcacatcga agatgaactc acctttgctg
     3481 ccgactcgcg cctgcgccat ctgcggctcg agagcggctt ctgcctcgag ctcgaaaaac
     3541 ccaccgcgct ttcccaccgc ggcggcagcg gcgttgaacg cgagcaacgt gctcgccgcg
     3601 ccgcaggcgg agattaaaaa atcgcggcga ccaataccca agcgcttggc attctggctc
     3661 gccgcttgat gcgccagccg gttggcggct cggttagtcg gcgagagcgg aaccggcgtg
     3721 aactcgccgt tcgaagtgct atcgagttta attggcagcc gctggccatc cggatcaatc
     3781 gtgtgtttca tcttgctgaa tatagactaa ccagcgggga aaaaaatagt tgctcatccc
     3841 atttataata attcgccgcg ctaaagccgg catattccgt tgacgacgag gatcgcggag
     3901 acgatcacag gtagctttat agccggaaat taacgggtgg ggcagaagct attcggtggc
     3961 acaaggcacc gtaaaccgga acgttgtccc acggccgacc gtgctctcga cccagatgtt
     4021 cccgccatgg gcatcgacaa tccctttgca gatcgccagt ccaagcccgg cgccatctcg
     4081 tgacgcgcgg tcacctcgcc aatagcggtc gaaaatgcgc ggtaggtccg cagcgggaat
     4141 gcccggcccg gagtcttcca ccgttatctg aaccgagtca tcgactttgc gcgcccggac
     4201 catgacccgg ccgccttcag gagtgaactt gaacgcattg cccaataggt tcgagagcac
     4261 ctgatccaag cgatcgcgat ctccattgac tgctggcagg ccggttggaa tatcggcagt
     4321 aacggtgatt ttttttgccc aggcctgtga ttcacatagt ttcagagact cctccagtag
     4381 cgcgccgata tcgacgtgcg aaggctggat ggagaaactg ccggcttcca tgcgggcgac
     4441 gtcgagcaaa tcacagatca ggcgatccat ggtttttacc gaactctgga tggcctcaac
     4501 gcaggtcttg atatccgccg acaacgccgg attggagata tcaatggctt tatccgcctg
     4561 tttatccagc gaatagccga tgattgttgc cgacgtgaga atggtgttca taggattgcg
     4621 cagatcgtgc accacaatcg ccataacctc atctcgcgct cgggtggcgc gctcggcctg
     4681 gctgcgcagc tgctgctcca aagcaagcgc tcgctcgcgc tcggcctcgg cgtttttctg
     4741 gtcggtgata tcggtgtttg tgccgagcca gttggtgatg cttccatctt cgccgcggat
     4801 tggcagagcc cgcgacagga accagcggta cgttccatcc ttgccgcgca gcgggtgggt
     4861 ctcttcccaa tcgtcccctg cctcgaagct gcgccgcagg ctctctacaa cccgttgcaa
     4921 gtgatccggg tgatgcgcct tgcgccaacc ccagccctgc gcctcttcaa aactggttcc
     4981 ggtgtaatcg taccatcgat cgttgaacca gtaagccgcg ccggtccggt cagccatcca
     5041 ggccagctgt ggaatggcat tgaccaacgt acggaagcgc gcctcttgcg ctcgcagctc
     5101 ctcttcggcg cgtcgacgag cctcagcggc gccggagagt tcgatgatgt gacgcaggct
     5161 cgatgccagc cgctccgctg tcagggacgc tttgggcaga taatccgccg cccccgcttt
     5221 catcaattcg acggctatat cttcatcgcc gcgcccagtg aagatcacca ccggcatatt
     5281 tgccgccact tccctgattt tctggaaaag tgccagccct ctgacgccgg gaatgtaata
     5341 gtcaagaaga acacagtcgt aaccgtttga agcaattcgt tgcagcgttt cctctgccga
     5401 agttgcatcc tcggcaatca cggggatacc gca
//
LOCUS       DASUTG010000736         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1447598, whole genome shotgun sequence.
ACCESSION   DASUTG010000736 DASUTG010000000
VERSION     DASUTG010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447598"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF089_17015"
     CDS             <1..213
                     /locus_tag="VF089_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6175714.1"
                     /translation="GNDRTSTFAIQQKVMESFHANGQPLEWHYFHFGNHGFASTESDG
                     YQPYLADLAWPLVTEFLGRELISPAL"
     gene            483..1196
                     /locus_tag="VF089_17020"
     CDS             483..1196
                     /locus_tag="VF089_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6175715.1"
                     /translation="MQVAEWRNTIGEMVRDHMRSPQLEHYFSVKMTKERAQLMITQLG
                     LYIRHRRDCWSLVSANCPVMAIKQAILKHEFGEVIKDEYSEFGHLHLIIRQAAKLGMS
                     AEEVLETKPIPSTTATLYAWAWLCHAKPWIEALAALTVTEWTNDDRLLKDIGAGHSTR
                     MGRRWMEDMAMAWRDMPNFVAHSQADEEHSDMFLPFLEEYAIGEKEERVLTAVQESLD
                     LFGMYREGVAQAMEKIPLK"
     gene            1221..1559
                     /locus_tag="VF089_17025"
     CDS             1221..1559
                     /locus_tag="VF089_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175716.1"
                     /translation="MEKPRIRHIAINVQDREKTAEYYKKVFAMEEKYRGPGGTIYLSD
                     GHLDLALISTEKYPWGINHFGFKVDSVKGIEEVAQTKAEENTFGAVAESWIKDAEGNR
                     VDVSEHGWPI"
     gene            1602..>2502
                     /locus_tag="VF089_17030"
     CDS             1602..>2502
                     /locus_tag="VF089_17030"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175717.1"
                     /translation="MSRLAKGARVGLARGGMGEARLAGVLFVAVFLLAFSRAAPAQTA
                     PQVTVAYAAVSPIFAGVWMAKEMGAFEKHGVKADLIYISSGSVTVQAMVGGNLDMSVA
                     ASNAVVSSILRGAPLVAVGSITNRPAMSLFVQPEITKPEQLQGKVLGITRHGSSTHFL
                     TQFVIEKLGLQDKVKIQPMGGTREVDTAFRTGMIAGAVRSMKPGPKVRMLVDLPDMGI
                     PFSMDLIAVKRDFYKSSPRTVEAMLKAYIEGVAGLRSRKDLAYKVLAKYLLGGGDSLD
                     EAYDYAVKYLDRNARTDPAVIQTV"
BASE COUNT          610 a          604 c          738 g          550 t
ORIGIN      
        1 ggcaacgatc ggaccagcac gtttgcgatc cagcagaagg tgatggaaag ttttcacgcc
       61 aacggacagc cgttggaatg gcattacttt catttcggta atcacggctt tgcttcgacc
      121 gaatcggacg gctaccaacc atatctggcg gatcttgcct ggccgctggt aactgaattc
      181 ctgggccgtg agttgatcag tcctgcattg tagaaagatt tcttgccagc ttcgcccggt
      241 tgcggtcata cggcgcgctg catcaaaggg aatgaacctg atcgatcttg gagtatcgag
      301 ttgactgcta gagaccagca catccaaagt tcatctccga cagatttata ggccaggtgg
      361 ttatggcgct acgttgctca acgacagatt gtataagagg ccttgagtaa ctaagcggtg
      421 atcggatata ggaaagacac gcggcccggg caaacgagat cctcacgtaa gggagatttg
      481 atatgcaggt tgccgaatgg cgaaatacga tcggagaaat ggtgcgcgat cacatgcgat
      541 cgccgcagct ggagcattac ttttcagtta aaatgactaa agagcgggcg cagctcatga
      601 ttacacaact gggactctac atccggcacc ggcgtgattg ctggtcgctg gtctcggcca
      661 actgcccggt gatggcgatc aagcaagcca ttcttaaaca cgaattcggc gaagtcatca
      721 aggatgagta ctcggagttt ggccaccttc atctgatcat ccgccaggcg gcaaagctcg
      781 gcatgagcgc cgaagaggtt cttgaaacca agccgattcc aagcaccacc gcgacgctct
      841 atgcctgggc ctggttatgc catgcgaaac cctggattga agctttagcc gcgctgactg
      901 ttaccgaatg gaccaacgat gatcgcttgt tgaaagatat cggtgccggc cattcgacga
      961 gaatgggtag gcgctggatg gaagacatgg caatggcatg gcgcgatatg cccaacttcg
     1021 tcgcccattc gcaagccgat gaagagcaca gtgatatgtt tctgccgttc ctcgaggagt
     1081 atgccatcgg tgagaaagaa gagcgggttc ttacggcggt gcaggagtca ctggatcttt
     1141 ttgggatgta tcgagaagga gtggctcagg cgatggaaaa gattcccctg aagtaaagct
     1201 ttcgaacaag gaggaccgag atggaaaaac cgagaattcg ccatatagcg atcaatgttc
     1261 aagaccgcga aaaaaccgcc gagtactaca agaaggtatt cgccatggaa gaaaagtacc
     1321 gcggtccggg cgggactatt tatctctccg atggtcacct ggatttagcg ctcatcagca
     1381 ctgagaaata cccctggggc atcaaccact ttggcttcaa ggtcgacagt gtcaaaggca
     1441 tcgaagaagt cgcccagacc aaagctgagg aaaacacctt tggcgccgtt gcggagagct
     1501 ggattaaaga cgccgaaggc aatcgtgtcg acgtttcgga acatggctgg ccgatttgat
     1561 ctgtgattaa gaaaagccgc tgcacttcag gagtacctga tatgagccga ttggcgaaag
     1621 gcgcacgcgt ggggttagcg cgtggcggaa tgggtgaggc ccgccttgcg ggcgttttgt
     1681 ttgtggctgt ttttttgctg gcattctcgc gagccgcgcc ggcgcagacg gcgccccagg
     1741 taaccgttgc gtatgctgca gtcagcccga tctttgccgg agtgtggatg gcaaaagaga
     1801 tgggagcttt tgagaagcat ggcgtcaaag ccgatctcat ctacatcagc tccggcagcg
     1861 ttacggtcca ggcgatggta ggcggcaatc tcgatatgag cgtggccgct agcaacgcgg
     1921 tagtcagctc gattctgcgt ggcgcgccgc ttgttgcggt gggcagtatt accaatcgac
     1981 cggcgatgag tttatttgtt caacctgaga tcaccaaacc ggagcagctt caaggtaaag
     2041 tcctgggcat tacacggcac ggttcgtcga ctcatttctt aacacagttc gtgatcgaga
     2101 agctgggact ccaggacaag gtcaaaattc aaccgatggg tggcacccgg gaggtggaca
     2161 ccgcttttcg gaccgggatg atagccgggg ctgtgcggag catgaagcct ggtccaaagg
     2221 tgcgcatgtt agttgatttg cccgacatgg gaattccctt ttcgatggat ctgatcgccg
     2281 tcaagcggga cttctacaaa agttctccga gaacggtgga ggcgatgctt aaggcttaca
     2341 tcgaaggagt cgcggggctt cggagcagaa aagacctcgc ttataaagtt ttagctaagt
     2401 atctcctcgg aggcggtgat tcactggacg aggcctatga ctatgccgtc aaatatttgg
     2461 accgtaacgc gagaacagac ccggcggtga ttcaaaccgt ac
//
LOCUS       DASUTG010000737         3175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_557336, whole genome shotgun sequence.
ACCESSION   DASUTG010000737 DASUTG010000000
VERSION     DASUTG010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557336"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="fabZ"
                     /locus_tag="VF089_17035"
     CDS             complement(<1..296)
                     /gene="fabZ"
                     /locus_tag="VF089_17035"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX6175718.1"
                     /translation="MLEVKDILKYLPHRYPFLLVDKILQIDGEEKIIAIKNVSFNENF
                     FQGHFPNRPVMPGVLICEAMAQVGAIFAHNARGGIEDNKVFVLTGLDNVKFKRPV"
     gene            complement(300..1364)
                     /gene="lpxD"
                     /locus_tag="VF089_17040"
     CDS             complement(300..1364)
                     /gene="lpxD"
                     /locus_tag="VF089_17040"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX6175719.1"
                     /translation="MPPRERILRDLAEYVGGRVIGDGSVTIYRVSPIEEAGPGDITFL
                     ANPRYARFIPGSKASAIIVSPQVVGSEVAKCGQGFLEVAQPYVAFAKILQLFAPATEY
                     RQEIAPSAHIEPTAKVGHDVSLYPNVYVGKLARIGDRSVLLPGVFVGDRVEIGSDCVL
                     HPNVVVRDGCRLGNRVILHAGVVIGSDGFGYAGSGEDRIKIPQVGVVEVEDDVEIGAN
                     TTIDRATLGRTLIARGAKIDNLVQIAHNVTVGANSVIAAQAGVAGSTKLGKNVTLAGQ
                     VGVVNHIEIGDGAVIGPQSGVPQSVPAGALLSGGIGVAPHHEWLKVMTLLPQLPRLWS
                     LVRRLEKQVAQLANRQPKEK"
     gene            complement(1367..1873)
                     /locus_tag="VF089_17045"
     CDS             complement(1367..1873)
                     /locus_tag="VF089_17045"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX6175720.1"
                     /translation="MKYWLVLFLILLCVPAVGAQERVKIGFIDIQRAISDSNAGKRAK
                     ERFQAQVKKAEAELLKEKTELERLKADLDKKGPLMKEEERRSLESDLQRRYVNYQRTM
                     TDQQQELRQKEGALTGDILKELEKIVNEIGKSDKFTLILERNQILYSDQGIDVTNKVI
                     EVYNSRNK"
     gene            complement(1926..>3175)
                     /gene="bamA"
                     /locus_tag="VF089_17050"
     CDS             complement(1926..>3175)
                     /gene="bamA"
                     /locus_tag="VF089_17050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX6175721.1"
                     /translation="TQSRNALQRTGYFEDVQLTTKKTDQPDTVDLLVDVKEGPTGTFT
                     VGAGFSSGDGFLFNASIAEKNLFGRGQGLNGSFAIGSTRQDFIVGFTEPYFNDSQMAL
                     GADAFNTEREFNDFDERKLGFGINTSYPLKDVSVPFYGRLRKRPDLGSDQLASNPAPS
                     IWDYMRGGMGYEFTRETINNIEATAPTTIQDERGTSLTSAMSPSISYDSRDHFFNPTE
                     GTKSAFSVKFAGLGGDNRFIKSDLSGRWHYPLLKDPNWGGAYVLALGGSLGYGVGLAE
                     RSNGQKDLPLFERYFLGGINSVRGFAERSLGPREPANCDDNGNCTDTEVVGGEKAMVL
                     SAELMFPIMEQFGLRGVAFFDMGNSFGESDKFSFTDLRRSVGFGARWMSPFGPLRVEL
                     GFPLNKQSGDETSVLGFTIGTQP"
BASE COUNT          732 a          909 c          779 g          755 t
ORIGIN      
        1 acggggcgtt taaacttcac attatccagg ccggtaagaa cgaagacctt gttatcttct
       61 atgccgccgc gggcgttgtg agcgaagatg gcgccgacct gggccatcgc ctcgcagatc
      121 aacactccgg gcatcaccgg gcgattggga aaatggccct gaaaaaaatt ctcgttgaac
      181 gagacattct tgatagcgat gatcttttct tctccgtcga tttgcaaaat tttatcgacc
      241 aaaagaaatg gatatcgatg gggaaggtat tttaggatgt ctttgacctc gagcatgagc
      301 tacttttcct ttggttgccg attcgcaagt tgtgccactt gcttttccag gcggcgcacc
      361 agactccata acctgggtaa ctggggcaga agggtcatca ccttcagcca ctcgtgatgt
      421 ggcgctacgc caataccgcc cgacagtaac gctcccgccg gcaccgactg aggcactcca
      481 gattgcggtc caatgaccgc tccgtcgccg atctcgatat gattgactac ccccacttgt
      541 ccagccaacg tcacattctt gcccaatttc gtactgcccg cgacccctgc ttgcgccgca
      601 atcaccgagt ttgctcccac cgtaacatta tgggcaattt gcacgagatt atcgatcttg
      661 gctccccggg cgatgagtgt gcggcccaga gttgcgcgat cgatcgttgt attggcgccg
      721 atctcaacat cgtcttctac ctcgacgacc cccacctgag gtatcttgat cctatcttcg
      781 ccgctcccgg cataaccgaa gccgtcgctg ccaattacca cgcccgcatg gaggataacc
      841 ctgttgccga gccggcatcc atcgcgaacc accacattgg gatgcagcac gcagtcgctc
      901 ccaatctcaa ctcgatcgcc cacgaacacc cctggaagca aaacactgcg atccccgatc
      961 cgcgctaact tgcctacata gacattgggg taaagcgaca catcgtgacc aaccttcgcg
     1021 gtcggctcaa tgtgcgcgga aggagcgatt tcctggcgat attcagtagc cggagcaaac
     1081 agctgtagga ttttggcgaa cgccacatag ggctgcgcga cctctaagaa accttggccg
     1141 catttcgcta cttcggagcc gacaacctgg ggagaaacga ttattgcgct ggctttggaa
     1201 ccgggaatga acctggcata gcgagggtta gcgagaaaag taatatcgcc ggggcccgct
     1261 tcttcaatcg gcgaaactct atagatggtg acggagccgt cgccgataac tcggccgccg
     1321 acgtactcgg ccagatcgcg aagtattctt tctcgaggcg gcacgtctat ttattgcggc
     1381 tgttgtaaac ttcgatcacc ttattcgtga catcgatgcc ttggtcgctg taaaggattt
     1441 ggttacgttc caagattaaa gtgaatttat cggattttcc aatctcattg acgatcttct
     1501 caagctcttt gaggatgtct ccggttaagg cgccctcttt ttgccgaagt tcctgttgct
     1561 ggtcagtcat cgttcgttga taattaacgt atcgtctttg cagatcactc tccaagctcc
     1621 gccgctcctc ctccttcatg agaggcccct ttttatccag atccgccttg agccgctcga
     1681 gctcggtctt ttctttaaga agctcagctt ccgccttttt cacctgcgcc tgaaatcttt
     1741 ccttggcccg tttaccggca ttggaatccg aaatcgcacg ctgtatgtcg ataaagccga
     1801 ttttgacccg ttcttgcgcg ccgactgctg gaacacatag aagaatcaaa aacaaaacca
     1861 accaatactt cacttaagcc tccagaaaaa tagaaagtag tgaacccggg ggacaagagc
     1921 accagctacg gctgggtgcc aatggtgaag ccgaggaccg aagtttcatc accggactgc
     1981 ttgttgagcg ggaatccaag ctcgacacgc aagggaccaa acggggacat ccatcgcgct
     2041 ccgaagccaa ccgatcttct aaggtcagtg aaactgaatt tatcagattc accgaacgaa
     2101 tttcccatat cgaagaaagc cacgcctcga agcccgaatt gctccatgat cggaaacatc
     2161 agctcggcgc tcagaaccat cgccttctcg ccaccgacta cttcggtgtc ggtgcagttg
     2221 ccgttgtcgt cgcaattggc gggttctcgc ggacccaggc ttcgttccgc aaaaccgcgc
     2281 acggaattga ttccgccgag gaagtagcgc tcaaacagcg gcaaatcctt ttgaccatta
     2341 gagcgttcgg ctagcccgac gccgtacccg agggatccac ccaaagccag cacataggcg
     2401 ccgccccagt tagggtcttt caaaagagga taatgccagc gaccgctcaa atccgatttg
     2461 atgaaacgat tatcgccgcc taatccggca aacttgacgg agaaggcgga tttagttccc
     2521 tcggtcggat taaaaaaatg atcccggctg tcgtagctga tgctcgggct catcgcgctg
     2581 gttaaggatg tgcctctttc gtcctgaatg gtcgtgggag ccgtggcctc gatattattg
     2641 atggtctcac gagtaaactc ataacccatt ccgccgcgca tataatccca aatcgagggc
     2701 gccgggttgc ttgccaattg atccgatcca aggtcaggcc gcttcctaag ccggccgtaa
     2761 aacggcacgc tcacatcctt caagggatag ctggtattga ttccgaaacc caactttctt
     2821 tcatcgaagt cgttaaattc ccgttctgta ttgaaggcat ccgcccctaa agccatctga
     2881 ctgtcgttga agtaaggctc ggtaaatccg actataaagt cttggcgggt ggagccgatt
     2941 gcgaaactgc cgttcagtcc ttgcccgcgg ccgaataaat tcttctcggc gatactggca
     3001 ttgaaaagaa acccgtcacc gctgctaaat ccagccccca ccgtgaatgt gccggtggga
     3061 ccttctttaa cgtccaccag aagatcgact gtgtccggtt gatcagtttt tttggtcgtt
     3121 agttgaacat cctcgaaata acccgtccgt tgaagcgcgt ttcggctctg agtga
//
LOCUS       DASUTG010000738         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_262345, whole genome shotgun sequence.
ACCESSION   DASUTG010000738 DASUTG010000000
VERSION     DASUTG010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262345"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF089_17055"
     CDS             complement(<1..305)
                     /locus_tag="VF089_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175722.1"
                     /translation="MKRQLIKVAPAIIFLSLLFSTSELVHAQQPVASFYYDDQGQVIR
                     QARDTNADGKMDRWSYYNGQGQIERIEQDMNFDGKPDALIYYENGKPRRQEVASNNDG
                     "
     gene            complement(337..540)
                     /locus_tag="VF089_17060"
     CDS             complement(337..540)
                     /locus_tag="VF089_17060"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6175723.1"
                     /translation="MFGAIGMPEQVIVSVIALMVFGPAKFPDFRKSLRATIRGLGKKI
                     SDIAECMSERSASPTPLNHVFRL"
     gene            747..2531
                     /locus_tag="VF089_17065"
     CDS             747..2531
                     /locus_tag="VF089_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX6175724.1"
                     /translation="MSVTTQTLINTGLEPESAKPPHRWLIGLLLAVCLTALFWQLGTA
                     GLFEPDEGRNAEKAREILLLNDWVTPHEDFFPVLDKPMFFYWLIAISYKAFGISEWAA
                     RLASVLAAVGCFALVYRFTRNWWGPWEALWSGLILISGVEFFILSRVVIFDMSLTFCI
                     TLALYSFFEAAHADETMRRRIYCALMYVGLAAGTLIKGLVGLLIPGLVIFFYLLIAKR
                     WSILRSLYLIPGALLFLALVVPWYLLAEARNAGFLRYYLWDEHFGRFATGSFDREQPW
                     YFFLVVLAVGFLPWSVLLPSVIKRNWSQSLDERRIFLLSWSIVPLIFFSLSSSKLAHY
                     ILPIFPALSILTGTTIAGMFQNRAYDVKRPLRWFWLMQSVNILYLALGLIWPAILPAR
                     IRDSLTPMAWSIAGYAVICLVILSALRWAKAAKLWQSQSTVFAVHAVGAFFFLIVLAQ
                     IMVAISPDRSAKEIARAVAAQADPETQVVLYDTYLSGMPFYLRAQRPVWIVTHANKKK
                     TVLGNFYVATNRAWPDTRWGKALFDLDEFREVWERKDMPLLIIVKQKNISRMEKEIGA
                     SPRRLTSVNEYVLMSNRHLADNPAHEHE"
     gene            2590..>3187
                     /locus_tag="VF089_17070"
     CDS             2590..>3187
                     /locus_tag="VF089_17070"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX6175725.1"
                     /translation="MEGWGVAVILGVVEGLTEFIPVSSTGHLIIVSHLLGFFGEKAAS
                     FQVAIQFGAILAVMLLYWRRFADLLPLGRNDRQCKNSNLAGWAGIWRITAATLPALVV
                     GYLTKDFIKTYLFSPWAVTVALAGGGVAILIAERILRRRASRPLETVTITQAIGVGLF
                     QVLALWPGTSRSAATIVGGMLLGLDRKGAAEFSFLIAVP"
BASE COUNT          659 a          804 c          858 g          866 t
ORIGIN      
        1 ccgtcgttgt tactagcaac ttcttggcgt cggggcttgc cgttctcata ataaatcaac
       61 gcatccggct tgccgtcgaa attcatgtct tgttcgattc gttctatttg tccttgccca
      121 ttataataac tccaacgatc cattttgccg tccgcgttgg tgtcgcgcgc ttgacggatc
      181 acctgtcctt ggtcatcgta gtaaaagctg gctaccggtt gctgggcatg aacgagctcg
      241 gaggttgaga atagcagcga gaggaaaatt atagctggcg cgactttgat tagctgccgc
      301 ttcatcgctc gagaactccg gactgctacg gcacaattac agtctgaaaa cgtggttgag
      361 cggcgttgga ctcgcggatc tctcagacat gcattcggcg atgtcgctta ttttttttcc
      421 taagccgcga attgttgcac gcagagattt tctgaagtca ggaaacttgg caggaccgaa
      481 gaccatgagg gcgatcaccg aaacgataac ctgctccggc attccaatgg ctccaaacat
      541 aaggcacccc ccgtaattag gggttcaagc tacgtcaggc cgcgagggtt gtcaatctga
      601 atccatgacc cattgacgaa gcttatatcg aagcatcggt cagtgtgcaa attcgcgtcg
      661 tttgatccgc aggcgatgac tgcgtatctt gcaattcagt gattgtcggg taaaccttgt
      721 cgaacacttc tttaagatca attcctgtga gcgttaccac gcaaactttg atcaatacag
      781 gcctcgagcc cgaaagcgct aagccaccac atcgctggtt gattggattg ctgttggccg
      841 tctgtctcac agctctcttt tggcagcttg gaactgccgg cttgttcgaa ccggacgagg
      901 gtcgaaacgc ggagaaagca cgggaaattt tactgctcaa cgactgggta acgccgcatg
      961 aggatttctt tccggttctc gacaaaccaa tgttcttcta ctggctaatc gcgatctctt
     1021 acaaagcctt cggtataagt gaatgggcag ccaggctagc ttctgtgttg gcggccgtgg
     1081 gctgttttgc gttggtctat aggtttacgc ggaactggtg gggcccgtgg gaagcgttgt
     1141 ggagcgggct gattctgatc agcggcgtag agtttttcat actgtctcgc gttgtcatct
     1201 tcgacatgag cctaacattc tgcattacgc tggcactcta ctcgtttttc gaagcagcgc
     1261 atgccgacga gacaatgcgg cgcaggatct actgcgcctt gatgtatgta ggattggctg
     1321 ccggtactct catcaaaggt ctcgttggat tgctcattcc tggcttggtc attttctttt
     1381 accttttgat cgccaagcga tggtcaattc tgcgttcgct ctatcttatt cctggtgcat
     1441 tattgtttct ggcgctcgtt gttccctggt atcttctggc cgaggcacga aacgccggtt
     1501 ttcttcgtta ctatctctgg gacgaacact tcggccgctt tgccactggg agctttgatc
     1561 gcgaacaacc ctggtatttc tttttggtgg tccttgctgt tggttttctt ccctggagtg
     1621 tgctgctccc gagcgtcatc aaacgcaatt ggtcgcaatc gttagacgaa cgaagaatct
     1681 ttttgctatc gtggagcatc gtcccgttaa tttttttttc gctgtccagt tcgaaactgg
     1741 cgcactatat tctgccgatc tttcccgcct tgtcgattct gaccggcacc acaatagctg
     1801 gcatgtttca gaaccgcgct tacgacgtca aacggccact gcggtggttt tggctcatgc
     1861 aaagtgtcaa cattctatac cttgctctcg gattgatctg gccggcgatc ctccccgcac
     1921 gtattcgcga cagcctcact ccgatggcct ggtccatcgc tggctacgcg gttatctgcc
     1981 ttgtgatttt gtcagcgctt cgctgggcta aagcagctaa actttggcag agccagtcaa
     2041 ccgtgtttgc cgtacatgcc gtaggagcat ttttcttctt aatcgttttg gcgcagatca
     2101 tggtggcgat ttcaccggat agatcggcca aagaaatcgc ccgtgccgtt gccgcccagg
     2161 ccgacccgga gacgcaggtt gtcttgtatg acacttacct tagcggaatg cccttttacc
     2221 tacgcgcgca aagaccggtt tggatcgtaa cccatgctaa caagaaaaag accgtgctcg
     2281 gtaacttcta tgtggccacg aatcgagctt ggcccgacac gcgttggggg aaggcgcttt
     2341 ttgatttgga cgaatttcgt gaggtttggg aaagaaagga tatgcctttg ctgattatcg
     2401 tcaaacaaaa aaatatctcg cgcatggaga aagagatcgg agcttcgccg cgcaggctga
     2461 caagcgttaa cgagtacgtt ttgatgtcga atcgtcacct ggccgacaat ccggcgcatg
     2521 agcatgaata gcaaggccct ggagtccgag cttttggtgc tacggcaagt gaactaacaa
     2581 ggggaataca tggaagggtg gggagtagcc gtaatcttgg gggtggttga ggggctaacc
     2641 gaattcatcc cagtatcatc gaccgggcac cttatcatcg tcagtcatct tctaggcttc
     2701 ttcggtgaga aagccgcaag ttttcaagtc gcgattcaat ttggcgctat tctagctgtg
     2761 atgttgctct actggcgtcg cttcgctgat ttgctgccgc tggggaggaa cgacaggcaa
     2821 tgtaaaaatt caaacctggc cggatgggct gggatttggc ggataaccgc ggcaacgctg
     2881 cctgccctgg tggtcggcta cttaacaaaa gatttcatca aaacctatct tttctcaccg
     2941 tgggcggtaa ccgtcgcgtt agcgggcggt ggggtagcga ttttgatcgc ggaacgcatc
     3001 ctcaggcgaa gagcatcgag gcctctggaa actgtgacga taactcaggc catcggagtg
     3061 gggctatttc aggttcttgc cctgtggccc ggcacctcac gatccgctgc cacgatcgtg
     3121 ggcggcatgc ttttgggtct tgaccgaaag ggagccgcgg aattttcctt tttgatcgcc
     3181 gtgccgg
//
LOCUS       DASUTG010000739         4069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_707079, whole genome shotgun sequence.
ACCESSION   DASUTG010000739 DASUTG010000000
VERSION     DASUTG010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707079"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1989)
                     /gene="glmS"
                     /locus_tag="VF089_17075"
     CDS             complement(160..1989)
                     /gene="glmS"
                     /locus_tag="VF089_17075"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6175726.1"
                     /translation="MCGIVGYIGSRDAAPLILESLRKLEYRGYDSAGIAVLNHGEVAI
                     RRCEGKLSNLATLLNRQPMSGPVGIGHTRWATHGRPSETNAHPHRAGDVVVVHNGIIE
                     NYLELKEQLTKRGTQFSSETDSEIVAHLVSEKIDRGMDFLDAVRRTLREIRGSYALLF
                     LNRRDPQRLIVAKNSTPIVIGWGNGETFIASDIPALLDYTRKVTFLEDGEIGEVTVGS
                     YRLFNGTGKAIRRPVKEITWDAVAARKGGYAHFMLKEIHDQPRALADTFRGRISLKNG
                     DVSLEAIRLTQSQVKRIKRIHLVACGTAWHACLVGKFMLEEIAGIPAEVDYGSEFRYR
                     SPLMDPQSILLMVSQSGETADTLAATEIARAKKAKILAICNVIDSSIARKSHGVLYTH
                     AGPEISVASTKAFSTQLTALYLLAVALGRLNGKLKQADARKLLRDLMHLPTWIEDALK
                     LEDEVKELARELMHSQDFLYLGRGLNYPIALEGALKLKEISYIHAEGYPAGEMKHGPI
                     ALIDEKMPVVVLAPRDRYFEKTIGNLKEVESRGGKVIVLTDDSKTSREVSAFRLLTLP
                     KASHYLTPIVMTIPLQLLAYHIAVHRGTDVDQPRNLAKSVTVE"
     gene            complement(1990..3384)
                     /gene="glmU"
                     /locus_tag="VF089_17080"
     CDS             complement(1990..3384)
                     /gene="glmU"
                     /locus_tag="VF089_17080"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6175727.1"
                     /translation="MNDIGVIVLAAGQGTRMKSDIPKVLHHLGGTPLFLHALRAAQRL
                     EPSRIAVIVGHGAGAVQQAYSGDDLCWVIQEKQLGTAHAVLCAEPTFHGFTGDILILS
                     GDVPLIRESTLKRLVDQHHREQAAVTLLTANLEDPKGYGRIARDDKQQITRSVEEKDA
                     SDLQKQIREVNAGIYVAAAPFLFSALSRVNDQNRQREYYLPDIVAIAARERRTVANLH
                     LDDAREMMGINTREDLARMEKSLQEEINRKWMAQGVTLKDPTTTYIEEGATIGKDTVV
                     GPNTHVCGTTTIGERCRIDGSAYLTDAHLGDEVHLRFSVVLSSCRVANGAIIGPFAHL
                     RPGTELGRNVHIGNFVEAKEARVGDGTKANHLTYLGDVTIGCDSNIGAGTITCNYDGF
                     QKYKTTIGDRVQVGSDSTLVAPISLGDDVYVATATTVRHDVPDGALVFNDRQERLREG
                     WTEQKRMKMKGIKK"
     gene            3617..>4069
                     /locus_tag="VF089_17085"
     CDS             3617..>4069
                     /locus_tag="VF089_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HEX6175728.1"
                     /translation="MTGSAILLLSLRGAIGLLRATLRVEVLHGEYLADLQSRRIPFLL
                     ALWHGRMFLIIDEHRGQGIVTMASKSNDGDIIAGWLEHNGYVVVRGSTTRGGSEALRR
                     MVYHMRAGRNGALTVDGPKGPVRVVQPGVVRLARLTGAWILPVSFSSAW"
BASE COUNT          883 a         1108 c         1081 g          997 t
ORIGIN      
        1 tttgagttgg cgtccggtta cttcatcaat accgtggcgc cggaaatcgc ggcacgtcga
       61 ctgcgcgagg ctgcgctatg acaaacttcc gttctgagtt tcggcacatg tttagagggc
      121 gtatgcaatc atgaaccaca attctcgcca ggagagcatt tattcaacgg tcacactttt
      181 ggccagattg cgcggctgat caacgtcagt gccgcgatga accgcaatgt gatacgccag
      241 caattgcagc ggtatcgtca taacgatggg cgtcaggtag tgcgatgctt tgggcagggt
      301 tagcaagcga aatgcagaga cttctcggct tgttttgcta tcgtcggtca ggacgatcac
      361 tttaccgccg cgggattcga cttccttgag attgccgata gttttttcga aataccgatc
      421 tcgcggcgcc aacacaacca ccggcatttt ttcatcgatc agcgcaatcg gcccgtgttt
      481 catttcgccg gcgggatagc cttcggcgtg gatgtaagag atttccttta gcttgagagc
      541 cccttcaagg gcgatgggat aattgaggcc gcggcccaga tagagaaaat cctgactgtg
      601 catgagctcg cgcgcgagtt ctttgacttc atcttccaac ttcaaagcat cttcgatcca
      661 ggttggtaaa tgcatcaagt cacgcagcaa ttttctagca tccgcttgct tcaacttccc
      721 gttgagtctt cctaatgcca cggccagcag atacagggcg gtaagctgcg ttgaaaacgc
      781 tttggtgctg gcaacgctta tttccggtcc agcatgggta tagagaactc catgcgactt
      841 acgcgcgata gaggaatcga tgacattgca gatggccaga attttcgctt tcttagcgcg
      901 cgcgatttcg gtggccgcca aggtatcggc cgtttcgccg gattgactaa ccatcaacaa
      961 gatcgattga gggtccatca gcggcgaacg gtaacggaat tccgagccgt aatcgacctc
     1021 cgccggaatt cccgcgatct cttcgagcat gaatttgccg accaagcagg cgtgccaggc
     1081 ggtgccgcag gcaaccagat gaatcctttt gattcgcttc acttgactct gagttaatct
     1141 gattgcctcg agagaaacgt cgccgttctt aagcgagatg cgacctcgaa acgtatcggc
     1201 tagagccctc ggctgatcat ggatctcctt gagcataaag tgagcatagc cacccttgcg
     1261 cgccgccacc gcatcccagg tgatctcctt gaccggccgc cgaatggctt tacccgtacc
     1321 gttaaagaga cgataagaac cgacggttac ttcgcctatc tccccgtctt ccaggaatgt
     1381 cactttgcga gtgtaatcga gcagcgcggg gatgtcggag gcaatgaacg tttcgccatt
     1441 gccccaaccg ataacaatgg gcgtggaatt cttcgcgacg ataagccgct gcggatctcg
     1501 gcggttaaga aacagcaacg cataggagcc gcggatttcg cgcaaggttc ggcgcaccgc
     1561 gtccaagaaa tccattcccc tatcgatttt ttcactgacc agatgcgcga ctatttcgct
     1621 atcggtttca gaactaaact gagttcctct cttggtcagt tgctctttca gttccaagta
     1681 gttttcgatg atcccgttat ggacgacgac cacatcgccg gcgcggtgcg ggtgagcgtt
     1741 ggtctctgaa ggacggccgt gggttgccca gcgcgtatgg ccgatgccga ctgggcccga
     1801 cataggctgc cggttgagca gagttgccaa attactcagt ttgccttcgc agcggcgaat
     1861 cgccacctcg ccgtggttga gaaccgcaat gccggccgaa tcgtaccccc ggtactcaag
     1921 ctttcgaagg ctttccaaaa ttaatggcgc cgcgtcgcgg ctgccgatat atccgacgat
     1981 accgcacatt tacttcttga ttcctttcat tttcatgcgc ttttgttcgg tccagccttc
     2041 gcgaagccgc tcttgtctgt cgttaaacac cagagctccg tccggaacgt catgacgcac
     2101 cgtggttgcg gtggcaacat agacgtcatc acccaaagag atcggcgcga ccaaggtcga
     2161 atcgctaccg acctgaacgc ggtcgccgat tgtggtttta tacttttgaa agccatcata
     2221 attgcaagtg atcgtgccag ctccgatatt gctatcacag cctatggtga catcgcccag
     2281 ataagtaaga tgattggcct tggtgccatc gccgaccctg gcctcttttg cttcgacgaa
     2341 attgccgata tgaacattcc ggcccaattc agtgccgggg cgcaaatgag caaatgggcc
     2401 aatgatcgcg ccgttagcaa ctcgacagct gcttaagacg acggagaatc gcaaatgcac
     2461 ttcatcaccc agatgcgcgt cagtcaggta tgcgctgccg tcaatgcggc aacgctcacc
     2521 gattgtggtc gtgccgcaga catgagtgtt cgggccaacg accgtgtctt tgccgatggt
     2581 tgcgccctct tcgatgtagg tggtcgtcgg gtccttcaag gtaacgcctt gggccatcca
     2641 tttgcgattt atctcttctt gtaagctctt ctccatgcgt gcgagatcct ccctcgtgtt
     2701 gatgcccatc atctcacgag catcatcgag atgtaggttt gccacagtcc tacgttcgcg
     2761 cgcagcgatc gcgacaatat cgggcagata gtattccctt tgccgattct gatcgttcac
     2821 gcgggataac gcagaaaaaa gaaatggcgc ggcagcgaca tagatgccgg cgttcacttc
     2881 acgaatttgc ttttgcaaat ccgaagcgtc tttttcttcg acgctacggg taatctgttg
     2941 cttatcatcg cgcgcgattc ggccgtaccc cttcggatcc tcgagatttg cggtcaataa
     3001 cgtcaccgct gcctgttccc tgtgatgctg atcgacgagc cgcttcaacg tgctctcgcg
     3061 gattaaaggc acatccccgc tcaggattag aatatcgcct gtaaacccgt ggaatgttgg
     3121 ttctgcgcaa agcaccgcat gggcggtacc gagctgtttt tcttgaatca cccagcataa
     3181 atcgtcgcct gaataggcct gttgaacagc gccggccccg tgcccgacta taaccgcaat
     3241 ccgggagggt tctagccgct gggcagcccg taacgcgtgc agaaacaacg gcgtgccgcc
     3301 cagatgatgg agtaccttag ggatgtccga tttcatccgg gtaccttgcc cggcggctaa
     3361 aacaatgacg ccgatatcgt tcataatgac taaggattat agcgaaagta ggttttcagt
     3421 caaagtaagc atacttagca agactgtacg ctgataactg gaatttgtcc ttttcgagcg
     3481 actaaattgg atttattata atcaactggt gctgcaacga ctgcaattca taccatactt
     3541 tgactaggac acttactttc cgtggctcgg gtgattcact agtgctttgc acgtcaattt
     3601 taaagagcca agaatcatga caggctccgc aattttgctg ctctcgctgc gcggcgcaat
     3661 tgggcttctg cgcgccacat tgcgagtgga agtgcttcac ggcgaatatt tggccgatct
     3721 gcagagccgc cgtatcccgt ttcttttggc gctatggcat ggacggatgt ttctcatcat
     3781 cgacgagcac cgtggccagg gaatcgtcac catggcttca aagtctaacg acggcgatat
     3841 tatcgcaggc tggcttgaac acaacggcta tgtggtggtc cgaggctcga cgacacgcgg
     3901 cgggagcgaa gctttgcgcc ggatggtgta tcacatgcga gcgggccgca acggagcctt
     3961 gacagttgac ggtcccaaag gcccggttcg ggttgtgcaa ccaggagttg tgcggctcgc
     4021 gcgattaacc ggcgcttgga ttctacctgt cagtttttca agcgcatgg
//
LOCUS       DASUTG010000740         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_409920, whole genome shotgun sequence.
ACCESSION   DASUTG010000740 DASUTG010000000
VERSION     DASUTG010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409920"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(851..1495)
                     /locus_tag="VF089_17090"
     CDS             complement(851..1495)
                     /locus_tag="VF089_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6175729.1"
                     /translation="MEIALYYAPNTCALAPYITLTEAGANFEVRPLNYRKREHFAPEY
                     LKINPKHKVPTLVVDGKILTENVAIHQWVHRTFPDAKILPGDPWHELQAISLHAWCAS
                     GIHPYLSRINNPGKVCDGPGASESVVKLATEALFENFQVADDLLAGREYFFDHFTAPD
                     AHFFWCCRRATQLKVDISGFPNVVAHFKRMQERTSVKKLLAYEKEVNEGFAKSA"
     gene            1796..>2290
                     /locus_tag="VF089_17095"
     CDS             1796..>2290
                     /locus_tag="VF089_17095"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX6175730.1"
                     /translation="MSTTMERPLADALTAIKTRRSVKEYLPTEIPREWIEELLDAAHW
                     APNHKLTHPWRFHVFTGEGRERLVAARQAAVRAAAERKGEPVSEDALNFARAKCFSSP
                     VVIIVSMVGDEKKIVDQENYAACWCAIENLLVAANARGLGSYPSTGDWIDQNFVGPVL
                     GLTEK"
BASE COUNT          539 a          606 c          639 g          506 t
ORIGIN      
        1 gcagagacgt tcgcgagcag actcgttatg ccatcgaacc cgtcaagatg attttgcaaa
       61 ctgcggggac ctctctcgat aacgtttctg acggtatcaa ctcatctaac caaacgtgag
      121 gaccttgcag cctacaacga ggagtacgcg aagtatttcc cgaccaacaa accagccagg
      181 accacggtgg aagcaatgtt gaacttcccg agctgcttgt ggaaataacc gtcacagctt
      241 gcgttcccga cgattaagac aggcgtcgaa ggaaagaaag aagacagctc aacctgcgct
      301 tttggagttc cgcagcaggg tcagttgtag ctgttcacta agctgccgat ctctagatca
      361 agctggaacg cctccgcact ccgaatctct ccaaggatct catttgaaca aattcatctg
      421 gcaagcagtg gcggcttgtt ttattgctcg ggtggtaaaa gccccgagca accgtccgtg
      481 gaatccgcaa gatagacgat cgtacgagct tggcttatca gcaccgcaat gagcgacacc
      541 agcgacaatc catgttgcgt cgctcaaacc gagaggcaaa ccttaatggg ccggcatcaa
      601 gacataggca tcgaaacagg tgatggccgt aatccgagag tcggatcaag gcgctgctac
      661 tcagtcagcg cgcgcggtga tcaccgatct gttaatttta gaaacgagag cgatcgtttc
      721 gcctatctcg aaaagggcaa ggcatttagc agcgcgctat cgatacggga tacgatcaga
      781 gttgagctgc tgtaatgcgc ccaacttaag tcactgactc atgtctgacc atgcgtgtcc
      841 ggataatcac ctaggccgac ttggcgaacc cttcattcac ttctttctcg taggcgagca
      901 gtttcttgac actcgtacgc tcctgcatac gcttgaaatg cgccacgaca ttgggaaagc
      961 ctgagatgtc caccttcaac tgggtggcgc ggcggcaaca ccaaaagaaa tgtgcgtccg
     1021 gcgcggtgaa atgatcgaaa aagtactcgc gcccggccag cagatcgtcg gcgacctgga
     1081 agttctcgaa cagagcttcg gtggccaatt tgaccacgct ttctgacgcg cccggcccgt
     1141 cacagacttt tccggggttg ttgatccggc tcagataagg atgaatgccg ctggcacacc
     1201 aggcatggag cgagatcgcc tgtaactcgt gccatggatc gccgggaagg atcttcgcgt
     1261 cgggaaacgt tcgatggacc cattgatgga tggcgacgtt ctcggtcagt atcttgccgt
     1321 cgaccacaag tgtaggcact ttgtgttttg ggttgatctt taagtactcg ggcgcgaaat
     1381 gctcgcgctt gcggtaattg agcggcctga cttcgaaatt cgcgcccgcc tccgtcagtg
     1441 taatataggg agcgagcgca caggtgttgg gggcgtagta gagagcgatt tccattcacg
     1501 tctccttgtt agctattgat caatgactac ttgcgaatct agtacccgcg tgaggtaagt
     1561 gcaacacatt aacgtccaaa aaaggttcca gcagttccaa cggtcccatt cgtgccagac
     1621 gttcaattcc gtatcggttc cggttacaat cagaagtctt gaccggcatg cccttaagca
     1681 ctcaaggtcg caaaagtagt ttgggaggac agcgcgcccg ctccgggatg ttgctcgccg
     1741 taaaaatttt ctatagatat ctgtaaatac tcggataatt caggaggtag gatctatgtc
     1801 aaccacgatg gagcgccctc tcgctgatgc gctcactgca atcaagacgc gccgctcagt
     1861 gaaggagtac cttcccaccg agattcctcg ggagtggatt gaggagctgc tcgatgcggc
     1921 ccattgggcg ccaaaccata aattaaccca tccgtggcgt tttcacgtgt tcaccggaga
     1981 ggggcgcgag cgtctggtcg cggcccggca agcggcagtg cgcgccgccg cggagagaaa
     2041 gggcgagccc gtgagtgaag atgctctcaa cttcgctcgg gctaagtgtt tttcgtcacc
     2101 ggtggtgatt atcgtttcta tggtcggcga tgagaaaaaa atcgtcgacc aggagaatta
     2161 cgccgcctgc tggtgcgcga tcgagaacct gctggtcgcg gcaaacgccc ggggcctggg
     2221 cagctatccc agcacggggg actggattga tcagaacttt gtcggtcccg ttctcggctt
     2281 gacggagaag
//
LOCUS       DASUTG010000741         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_707132, whole genome shotgun sequence.
ACCESSION   DASUTG010000741 DASUTG010000000
VERSION     DASUTG010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707132"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            676..1251
                     /locus_tag="VF089_17100"
     CDS             676..1251
                     /locus_tag="VF089_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175731.1"
                     /translation="MENQQSQTVASGSDGGAIDKVKSEAAGVAQDVKRQGQEQFEARK
                     QTAADQTEKLAGVVDRVSEELKGQNQESLADYAGQLAGSMKNFAESLRQRNLDELVND
                     TQQLARSNPTLFLMGSVAVGIALSRFLKASADRSDSNRSNDSEFYDSSRSVVQPITFE
                     SRPSVTSESATTTPSSKSDSPSFADDLEKGV"
     gene            1254..1691
                     /locus_tag="VF089_17105"
     CDS             1254..1691
                     /locus_tag="VF089_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6175732.1"
                     /translation="MAKQPPFQATPATESSRVDREPDSTAGLLSRLMGEVSTLFRKEI
                     ALAKAEVSEAASEAKAGAISLVAGGAILFAAVLVLLAAIVLLLAERMDPWLAALIVAV
                     VVGVIGFVMIQSGKKKLDPSSFKPERTQDALRKDKEMVQRRAS"
     gene            1688..>2216
                     /locus_tag="VF089_17110"
     CDS             1688..>2216
                     /locus_tag="VF089_17110"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX6175733.1"
                     /translation="MSNVNNTTNLRDDARKDPATLEREIDQTRANMDRTLGALERKFS
                     PGQLLDQAMEFARENGGEFANNLGRSVKENPVPAILTAVGIAWMVASSNRPRSWTADA
                     YDERYTGNDFNRLEFDQAVTEGDAGDEDEKEGLTEKAQRLKASAEGTLTEAGQRVKSA
                     AERTRQKLASTNETMS"
BASE COUNT          575 a          583 c          615 g          443 t
ORIGIN      
        1 cgctttatcg agcaatcgct ataccaggaa aattccttag gcacaggcgt cttcgtattg
       61 cgcgcgagac aaaaacattt tcagttctct ataactgcga catgcaggaa atgccggcca
      121 cctggattaa tttcagaggt gtattccgga gggcttacgg acttgttaag ttactttcat
      181 gatgaaaaca cacaaagcgc tacgctccga taccttcccg gcacccatag agttgcacga
      241 ggtggacggc attcctctgt ccgcagaaga agtgaatctt gcaaagctgc taattgataa
      301 gctcgcgcgg tctgcaaagg gaagcctgat cgacttcaat cgagcatcta agcggcgctt
      361 taacccatcg agctcacgaa aacgttagct gcttgagtcg ccccggatca tcgatcgcca
      421 tacatcctcc ctcacgctac ccagcaatct accgcagact cgcccgcctg agcgaaacct
      481 gattcgtccc ttcgatgcac ttgccccgat cgatgtttcg cagccctgcg acattgatcg
      541 acctgtttcg agctaaatgc gcgagttacg tagtggcata accattgcgt ttgtccactg
      601 cattcccacc aggtcaacgc ttaatccaag atcaaaattt ctcagcgaga gtatttttac
      661 gaaggagtat tatctatgga gaaccagcaa agccaaaccg ttgcgagtgg aagcgacggc
      721 ggcgcaatcg ataaagtaaa gagtgaggct gccggagtgg cgcaagatgt aaagcgccaa
      781 gggcaggaac aattcgaagc gcgtaaacag acggcggcgg atcaaactga aaagctggcc
      841 ggcgtggtgg accgcgtctc cgaagaactc aagggccaga atcaagaatc gctggctgat
      901 tacgcaggtc agctggcagg cagtatgaag aatttcgctg aaagcttgag gcagcggaac
      961 ctcgacgagc ttgtcaatga cacccagcag ctagcgcgca gcaatcccac ccttttccta
     1021 atggggagtg tagcggtcgg cattgcgctt tcgaggtttt tgaaagcatc ggctgaccgc
     1081 agcgactcga accgaagcaa cgattcagag ttctacgatt cgagccggtc ggtagtgcaa
     1141 ccgatcacct ttgagtcgcg gccgtcggtc acatcggaat cagcgacgac aacgccatct
     1201 tcaaaatcgg acagcccgtc tttcgctgac gatctagaga aaggagtttg atcatggcaa
     1261 aacaaccgcc atttcaagcc acgccggcaa ccgagtcatc tcgcgtcgac cgtgaacccg
     1321 attcaacagc aggcctgcta agtcgtctca tgggggaagt gtcgacgcta tttcgcaaag
     1381 agatagcgct ggcaaaggcg gaagtatccg aggccgcttc agaagcaaag gcgggagcca
     1441 tcagccttgt agccggcggc gcgattctct ttgccgccgt cctcgtcctg ctggccgcga
     1501 ttgtcctgct gttagccgaa aggatggatc cttggttggc agctctcata gtcgccgtgg
     1561 tggtcggcgt cattggcttt gtaatgatcc aaagcggcaa gaagaagtta gatccctcgt
     1621 ctttcaagcc tgagcggact caggacgcgc tgcgcaaaga caaggagatg gttcaaagga
     1681 gggcctcgtg agcaacgtaa ataacacaac caacctaaga gatgatgctc gaaaagatcc
     1741 ggctaccctg gaacgagaga tcgatcagac ccgggccaac atggaccgaa cactcggcgc
     1801 attggaacgc aagttttccc cgggtcaact gctggatcaa gccatggaat tcgcgcgaga
     1861 gaacggcggc gagttcgcta ataatctcgg tcgatcggtg aaagagaatc cggtaccggc
     1921 gatattaacg gcggtgggaa tcgcctggat ggtcgcgtcg agcaatcgac ccagatcgtg
     1981 gacagccgat gcttacgacg agcggtatac tggaaacgat ttcaaccgtc tcgagttcga
     2041 tcaagcggtt accgaaggtg atgcaggcga tgaggatgag aaggaaggat tgaccgagaa
     2101 ggcgcaacgc ctcaaagcct cggccgaggg aacgttgact gaagcagggc agcgggtcaa
     2161 gtccgccgcc gagcgaactc gacagaaact ggcaagtact aatgagacaa tgtcat
//
LOCUS       DASUTG010000742         8125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_854373, whole genome shotgun sequence.
ACCESSION   DASUTG010000742 DASUTG010000000
VERSION     DASUTG010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854373"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF089_17115"
     CDS             <1..646
                     /locus_tag="VF089_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6175734.1"
                     /translation="GVGKSTLADLIPRFYDVTSGAITMDNIDIRDLTLRSLRQQIGLV
                     SQHTFLFNETIGNNISYGDPGRGLDDIIAAAKAAYAHDFIMALPRGYDTEIGEMGLQL
                     SGGQRQRLAIARALLKDAPILILDEATSALDAESERSVQNALDNLMTRRTTVVVAHRL
                     STIRRADRIVVLVGGEIAEQGSHQELMARQQEYSRLYSMQLLEDARPVEPKVMH"
     gene            660..1952
                     /locus_tag="VF089_17120"
     CDS             660..1952
                     /locus_tag="VF089_17120"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX6175735.1"
                     /translation="MGYLVYNALITLAFLISLPVLPLLLLGGERFRAGLGQRLGWYSI
                     DNTIPPKAPRPIWIHAASVGEVRSVRALTQQLKRLFSDRAIIVSTLTHTGNLTARQNP
                     AIDRVIYLPLDLWWTVRRALAVFDPAVLIFIETEIWPNLLRQAHRKGLPTILLSGRVS
                     EKGFNSYCRLAWFFKGVFQLLRACGMQSEIDRQRIVRLGAAPNRVSVTGSLKHAAVLS
                     DGTGAGDHVSEQRFAGSWIWVVGSSHRGEEEIVCAAFKTLKQQFPGLRMILAPRHPQR
                     FAEVEKLLVAGGVSFEKKSSIGTGHEFQHDVLFLDTIGDLERFYADADFAFVGGSLVD
                     AGGHNLLEPARLGKPVLFGPYTSSVTLLAHNLKQNGGGFEVGSAEDLVREIAGLLSDP
                     QKRRLAGEKAYEVAATNNTVIDQSLNIVAIYLPASAAT"
     gene            1989..3092
                     /gene="lpxK"
                     /locus_tag="VF089_17125"
     CDS             1989..3092
                     /gene="lpxK"
                     /locus_tag="VF089_17125"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX6175736.1"
                     /translation="MRSVGIQRVWQRRGASGKLLWLLLLPASWAYRFAVALRNLSYRW
                     KCARVRALNCPIISIGNLSVGGTGKTPATLWLAQRLMERGFKVGVLSRGYKRRGSGTI
                     ILSGDSGVSSATIDGSEALAAGDEPVMMARIYELTVSVAKNRYQAGVELLRKKDLDVL
                     ILDDGFQHRKLKRDLDLVLLGEDVPGWLLPCGPFREPTRSLRRADCFLVTGAAERWQR
                     WIQANGGRPSFQAMLRPQALIGFESDRCKEYPLTLMYRSKILALAGIAKPANFYQTIH
                     EYDGEIVDTLEYPDHHDYSATDWQQINRIARNVDLIVTTEKDILKLIRFPFARGKLLA
                     LRVSMTVMQEEGLLDLAIGSIRNHARHQNESRT"
     gene            3199..4140
                     /locus_tag="VF089_17130"
     CDS             3199..4140
                     /locus_tag="VF089_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017662991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6175737.1"
                     /translation="MKVLVTGVAGFVGSHLAEKLLSENYDVVGIDKFLDNYPRRFKQK
                     NLGSFLKHANFQFLEEDLLQVNLGELLKDVSYVFHLAAQPGVRSSWGDEFIEYSQNNI
                     MATQLLLEAGKESRVKKIVYASSSSVYGDTDDLPMREEGNTRPVSPYGVSKLAAEHLC
                     YLYWKAYGVPTVSLRFFTVYGPRQRPDMFFHIFMDRLRKNQEVPLYDDGEQTRDFTYC
                     GDIVDGLLGAALYPGAGEVFNLGGGTESSVLNAIHIVEKISGRKAKLKTFERQKGDVR
                     HTRARLDSATEKLSYRPKVSLEEGLSQQWRWIQTLDG"
     gene            4195..5055
                     /locus_tag="VF089_17135"
     CDS             4195..5055
                     /locus_tag="VF089_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175738.1"
                     /translation="MLDYTEEDVGNIVSLEHVNLQVPDQALATLFYIVGLGLTRDPYL
                     NVGLGNMWANIGEQQLHLPTRSAQRISGNIDLVLPDLKALAERLSSIAPALKSTQFSW
                     SSNQDYLAVTCPWGNRFRCFESGPVFGDMAQGLAAVDFTVREGTAKAIAAFYHNVLGA
                     PATVETDAGHAVAYVKIGRNQTLRFRESEEPIPPYDGHHIAIYVANFSRPYNLLKTHQ
                     LISEDVRNHQFRFQSIIEVDSGRTVFLLEHEVRSLHHPMFNRHFVNRDPAQSQRNYHR
                     GRDALIPFRA"
     gene            5118..5285
                     /locus_tag="VF089_17140"
     CDS             5118..5285
                     /locus_tag="VF089_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6175739.1"
                     /translation="MAISKDLLDILACPKCKGDIHLNDRGDGLICDACRLMYPIKDDI
                     PVMLIDEAEHI"
     gene            5289..6386
                     /gene="waaF"
                     /locus_tag="VF089_17145"
     CDS             5289..6386
                     /gene="waaF"
                     /locus_tag="VF089_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX6175740.1"
                     /translation="MLPANWENVLVVQTSFLGDTVLTLPLLSEIKRRYPAGKLTLLCA
                     PVAAELLGSHEAIDEVLIDDKRRQDAGVLGLWRKAKVLEQKRFTLAISPHKSLRSALL
                     LFLARIPRRVGFRQSQGAFLFHDLADRNPGCHDVERNLSILQPLGIPTETCRRDIDLP
                     VAEAAREQSRHLLQALGVDLNKTVIGINAGSVWPTKRWAPDLFARLITKLKQNLNCEI
                     VLFGGPEDHNTVATIQALCETEVVNLSGKTALRDLPAAISACDLFITNDSGPMHIAVG
                     CGVPVVAIFCATTPSLGFYPYSSKAVVVQKELPCRPCSSHGGRRCPLGTEDCIKLIRP
                     EEVLQAAIQMLQVKDRMPPDSSTHAPQYLNL"
     gene            6429..7202
                     /locus_tag="VF089_17150"
     CDS             6429..7202
                     /locus_tag="VF089_17150"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6175741.1"
                     /translation="MNLLSDERIWGAAIDGFTRMTAEPRRRFLLRSDVADQIHIDIFR
                     GDPHPGQLSRFAGREALRSINLKNGENALIRTYRHGGVFRWITGKTFFSWPPRPFREL
                     AITEELHRRGVPTVEVYGAGVEGLFGPFYRGWLITRELKDSYDLWTALQNGFVRKWTF
                     EKVFRSVATSLRSLHREGVYHSDLNLKNILVKAEPAGVKGYIIDFDKAVLFVGGLPEP
                     LAKKNLRRLLRSALKLDPQRQYLTAAEWDKLLTWYHEAR"
     gene            7307..>8125
                     /locus_tag="VF089_17155"
     CDS             7307..>8125
                     /locus_tag="VF089_17155"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX6175742.1"
                     /translation="MGLISQLKYCHLREDGWSLSVDSGIWSRALWLAILAKIQEQVPS
                     RHPQTIKLELPESQQQSYFLKVYHSASGWGAVKDWFRASKALRFLKQGEALANAGFLV
                     PPAVAAGEKRHFGMLKAAFVLTAAIEGAPLTNFLKENAGRTDAGLSLIEKRATLKALA
                     RQIRRFHDLGFVHGDLVASNIFVCRDAAMNLQFVFMDNDRTRRYSKWLPQSLWKRNLV
                     QLNRLPLSSISLQDRIRFFQEYSTAEGAADLNRPLLRWLEQKTRERRRECDSVDA"
BASE COUNT         1863 a         2053 c         2260 g         1949 t
ORIGIN      
        1 cggggtcggc aaaagtactc tggccgacct gattccgcgc ttctacgatg tcacgtccgg
       61 cgccatcact atggacaaca tcgatattcg cgatctcacg ttgcgatcgt tgcggcagca
      121 aatcggtttg gtaagccagc atacctttct gtttaacgaa acgataggca acaacatcag
      181 ctatggcgat ccgggtcgcg gcctggatga tatcatcgcc gccgccaagg cggcctatgc
      241 ccacgatttc attatggcgt tgcccagggg ctatgatacc gagattggcg aaatggggct
      301 gcaattatcc ggcggtcaaa ggcagcgtct ggctatcgcg cgggcactgc tgaaggatgc
      361 gcctattctc attctggacg aggcgacctc ggcgctcgac gccgaatcag aacgatcggt
      421 acaaaatgct ctcgataacc tgatgactcg gcggaccacc gtagtggtgg ctcaccgatt
      481 gtcgacgatt cgccgggcgg accggattgt ggttttggtt ggcggagaaa tcgctgaaca
      541 aggtagtcat caggaactga tggcgcgtca gcaggaatac agccgtctct acagtatgca
      601 gttgctggaa gatgcccgac cggtcgaacc gaaggtaatg cactgaacca cggtcgacga
      661 tgggctattt agtttacaat gcgcttatta cactggcttt cttgataagc ttgccagttc
      721 tgccactgct gctgttggga ggagagcgtt tccgggctgg tcttgggcag agactggggt
      781 ggtattcaat cgataataca attccaccca aggctccgcg acccatatgg atacacgcgg
      841 cttcagtcgg ggaggtcagg tcggtgcgcg cgttaaccca acaacttaag cggctctttt
      901 ctgatcgtgc tatcatcgta tccactttga cacatacggg aaatctgacc gcgcgtcaga
      961 atccagcgat tgatcgagtt atctatctac ccctggacct ttggtggacg gtgcgacgag
     1021 cactggccgt tttcgatcca gctgtgttga tcttcattga aactgaaatc tggcccaact
     1081 tgttgcgtca agctcatcgc aaaggattgc cgacaattct tttgagtggg cgcgtctcag
     1141 agaagggctt caacagctat tgccggctgg cttggttctt caaaggagtg tttcaattgc
     1201 tgagagcctg tggcatgcag tcggaaatcg accgacagcg gattgttagg cttggggcag
     1261 cgccgaaccg ggtctcggtt actggcagcc ttaagcatgc cgctgtgctg agtgacggaa
     1321 ctggtgcggg ggatcacgtg agcgagcaaa gattcgccgg cagctggatt tgggtggtag
     1381 gtagctcgca tcgaggagag gaggagattg tctgtgcggc gttcaaaaca ttgaagcagc
     1441 agtttccagg gctgcgtatg attctagcgc cacgtcatcc gcaacgtttt gccgaagtgg
     1501 aaaaactgtt ggttgccggt ggagtctcat ttgaaaagaa aagtagcatc ggaaccgggc
     1561 acgagtttca gcatgacgtc ctctttctcg acaccatcgg cgacttagag aggttttatg
     1621 ccgacgctga tttcgctttt gtcggcggca gcctggtaga tgcaggcggc cataatcttt
     1681 tggaaccggc tcgcttgggc aaaccggttc tattcggtcc ctatacttcg agtgtgacgt
     1741 tgctggcgca caacctgaag caaaacggcg gaggctttga agtagggagc gccgaagatt
     1801 tggtgcgtga aatagccgga ctgctttcgg atccgcaaaa acgccgacta gccggtgaga
     1861 aggcgtatga ggtcgctgcc acaaataaca cagtgatcga tcaaagcttg aacatcgtcg
     1921 cgatctatct acccgcgtcg gccgctacct aatttcatcg gccttttcgg acacacgtta
     1981 gagccttgat gagaagcgtt ggcatccagc gcgtttggca acgaagagga gcatcgggca
     2041 aacttctgtg gctcttgctt ttgcctgcgt cgtgggctta ccggtttgct gtagctcttc
     2101 gcaatctctc gtatcgatgg aagtgcgccc gggttcgcgc actgaactgc ccaattatca
     2161 gcattggcaa cctttcggtc ggcgggaccg gaaaaactcc ggcgacctta tggttggcgc
     2221 aacgcttgat ggaacgcgga tttaaggtag gtgtccttag ccgcggttat aaacgaaggg
     2281 gatcgggaac gatcattttg tccggcgata gcggcgtatc ttctgccacg atcgatggct
     2341 ctgaagcgct cgctgccggc gatgagccag tcatgatggc gcgcatctat gagttaactg
     2401 taagtgtggc caaaaatcga tatcaggccg gcgtggagct actgaggaaa aaagatctcg
     2461 acgtccttat tctcgatgac ggctttcagc atagaaaact caagcgagat cttgatctgg
     2521 tgctgttagg ggaggacgtg ccgggatggc ttttgccttg cggtccgttc cgtgaaccga
     2581 ctcgctctct caggcgggcc gactgttttt tagttacagg agctgccgaa cgctggcaga
     2641 gatggattca ggcgaacgga gggcgtccct ctttccaagc aatgcttcgg ccacaagcgc
     2701 tcatagggtt cgaatccgat cgatgcaaag aataccccct aacactaatg tatcgcagta
     2761 aaattctggc gcttgccgga atcgcaaagc cggcaaattt ctatcagacg atacatgagt
     2821 atgatggcga gatcgtcgat actttagaat acccggacca tcacgactac tcggccacag
     2881 actggcaaca gatcaatcgc atcgcgcgca atgtcgatct catcgtgacc accgaaaagg
     2941 atattctaaa gttgattcgc ttcccttttg cacggggtaa attgttggcg ctacgtgtat
     3001 cgatgacggt gatgcaggaa gaaggtttac tcgacctcgc catcggcagt atccgtaacc
     3061 atgcccgtca tcagaatgaa tctcggacgt aatcgcagtc tcagcgctgt cagtgcagtg
     3121 ctctattggc gaggcatcgc cgaacgggac ggacgagtcg ggcacggcta tcagtcattc
     3181 agctagcagg aggctaacat gaaagttcta gtcacgggag ttgccggctt cgttggttca
     3241 catctggcag agaaactgct cagcgaaaat tatgacgttg tcggaatcga caagttcttg
     3301 gataactatc ctcgtcgatt caaacagaag aacctcggat cgtttttgaa acacgcgaat
     3361 ttccaatttc tcgaagaaga tctgttacag gtaaatctcg gcgagctctt gaaggatgtg
     3421 tcctatgtct ttcatttagc ggcccaaccc ggagttcgct ccagctgggg tgatgaattc
     3481 atcgagtata gccagaataa catcatggcg actcagctgc ttctggaagc cggtaaagaa
     3541 tcccgcgtta aaaaaattgt ttatgcatca tcctcctcgg tgtacggcga tactgacgat
     3601 cttcccatgc gcgaggaggg caatacccgg ccggtatccc cctacggcgt cagcaagctg
     3661 gcggccgaac acctttgtta tctttactgg aaagcgtacg gcgtgccaac cgtatcgctg
     3721 cgctttttta ccgtatacgg gccgaggcaa agaccggaca tgttctttca cattttcatg
     3781 gatcgtcttc gcaagaatca ggaagttccg ctctatgatg acggcgagca aacccgtgac
     3841 tttacttatt gtggcgacat cgtcgatgga ctcctcggag cggcgcttta tccgggcgcc
     3901 ggggaagtgt tcaaccttgg cggcggcact gaaagctccg ttctcaatgc catccatatt
     3961 gttgagaaaa tatccggacg caaggcaaag ctaaagacct ttgaacggca aaaaggcgat
     4021 gtgcgccaca cgcgagcgcg acttgattcg gccactgaga agttaagcta tcgccccaag
     4081 gttagtctgg aggaaggctt aagccagcaa tggcgctgga ttcagacgct tgacggttga
     4141 taggatatgt catgaccaga tcgttgtctg acttaccgga agcaggagag cagtatgctt
     4201 gattacactg aggaagacgt cggcaatatc gtatcactgg agcacgtcaa tttgcaggtg
     4261 ccggatcagg ctctggcgac gctattctat attgtcggcc tcggtttgac ccgcgatcca
     4321 tatctgaacg tcggcctcgg caatatgtgg gctaacatag gcgaacagca gcttcactta
     4381 cctaccagat ctgcacaaag aatctcgggt aatatcgatc tcgtcttgcc tgatctgaaa
     4441 gccctggcgg aaaggctttc gtcaatagca cccgcgctca aatccacgca gttttcatgg
     4501 tccagtaacc aggattacct tgccgtcacc tgtccgtggg gcaaccggtt tcgctgtttc
     4561 gagagcggcc cggtcttcgg cgatatggcg cagggacttg ccgctgttga cttcaccgtc
     4621 cgcgaaggaa ctgcaaaggc tattgctgcg ttctaccaca acgtacttgg agcgcccgct
     4681 actgttgaaa ctgatgcggg ccatgcggtg gcgtatgtta aaattggacg taatcaaaca
     4741 ctccgatttc gggaatcgga agaaccgatc cctccttacg acggccacca tattgctatc
     4801 tatgtggcaa acttttcgcg gccgtataat cttcttaaaa ctcatcagct aatatccgag
     4861 gacgtgcgca atcatcagtt taggttccaa tcgatcatcg aagtcgatag cggcaggact
     4921 gtgtttcttc ttgagcatga ggttagaagc ctgcaccacc ctatgttcaa ccggcatttc
     4981 gtcaatcgtg acccggcgca gagccagcgc aattatcatc gcggccggga tgcccttata
     5041 ccttttcgcg catgatgatg attggaacgg cttgcacacg attggggctc attaattagc
     5101 agcttaaagg gggaattatg gcgatcagca aggacttgct cgatatcttg gcgtgtccga
     5161 agtgcaaggg cgatattcat ctaaatgatc ggggcgacgg tcttatctgc gatgcgtgtc
     5221 gactcatgta tcccataaag gatgatatcc cagtgatgct gattgacgaa gccgagcata
     5281 tctagcggat gctccccgcc aattgggaaa acgtcctggt cgtccaaacc agctttctag
     5341 gcgacacggt cctgactttg ccgttgcttt ctgaaatcaa gcggcgctat cccgctggaa
     5401 agctgactct gctttgtgca ccggtcgcag ccgaattgtt ggggtcgcat gaggctatcg
     5461 atgaagtctt gatcgatgat aaacgacgcc aggacgccgg cgtcttggga ttgtggcgta
     5521 aagccaaagt tctcgaacaa aagagattca cgcttgccat ttctccgcac aaatcgctgc
     5581 gcagtgcttt attgttgttt ttagccagaa ttccccggcg tgtcggtttt cgacagagtc
     5641 aaggagcgtt cttgttccac gatttggccg acagaaaccc gggatgtcat gatgttgagc
     5701 ggaatctttc catcctgcag ccgttgggga ttcctaccga gacgtgccga cgggacatcg
     5761 atcttccggt ggctgaggcc gccagagaac agagtcgcca cttacttcag gccctcggcg
     5821 ttgatctgaa taaaactgtc atagggatca atgccggttc tgtatggccg actaaacggt
     5881 gggctcccga tctgttcgcg cggttgatca caaaacttaa gcagaatttg aattgcgaaa
     5941 tcgttctttt cggcggtccg gaggatcaca acacagtcgc aacaatccag gcgctttgtg
     6001 agaccgaagt cgtgaacttg tccggtaaaa ctgcgctacg agacttgccc gcggcgatca
     6061 gcgcatgtga tctgtttatc actaacgata gcggcccaat gcatattgcc gttggctgcg
     6121 gcgttccggt ggttgcaatt ttttgtgcta caacgccgtc tctggggttt tacccataca
     6181 gttccaaagc ggtggtagta cagaaggaac tcccgtgccg tccctgcagc tctcacggcg
     6241 gtaggcgctg tccgttggga acggaagatt gcatcaagct catccggcca gaagaggtat
     6301 tgcaagcagc catacagatg ttgcaagtca aggatcgaat gccgccagat tcgagcactc
     6361 acgcgcctca gtatctcaat ttgtaagagc ccctcattct ctctggctca gcccagaggt
     6421 ggcttaggtt gaatttgtta agcgacgaac gaatctgggg cgcagccatc gatggcttca
     6481 cgagaatgac tgctgagccg cgccgccgct tcctgcttcg ctccgacgta gcggatcaaa
     6541 ttcacatcga tatattccgg ggagatcctc atccaggtca actgagccgg tttgcaggaa
     6601 gagaggcact aagaagcatc aacttaaaaa acggcgaaaa cgctctcatt cgtacttacc
     6661 gccatggcgg ggttttccgt tggatcaccg gtaagacatt tttctcctgg ccgcctcggc
     6721 cgttccgaga attggcgatc accgaggagt tacatcgccg gggtgttccg acggtcgagg
     6781 tgtacggcgc aggcgtggag ggtcttttcg gacctttcta ccgcggttgg ctgattaccc
     6841 gagagttgaa ggactcctat gatttgtgga cagccttaca aaacggcttt gtccgtaaat
     6901 ggacattcga gaaggttttt cgatcggtgg caactagcct gcgctcgctc catcgcgagg
     6961 gcgtttacca cagtgatcta aatcttaaga atatactagt gaaggcagag ccggccgggg
     7021 tgaagggata tattatcgat tttgacaagg cggtgttgtt cgtcggaggc ctgccggagc
     7081 cgcttgcgaa aaaaaatctg cggcgcctct tacgttcggc attgaagctc gatccacaac
     7141 gtcaatattt gaccgcggcg gaatgggaca agctattgac ctggtatcat gaggcgcgat
     7201 agccatgagc gtagcaccga gcagtatacg ttcgtgctgc gggactcgat cggcgccaaa
     7261 gagatccggg ccaaggatag tttttgatta acgcgcagcc taaggtgtgg gattgatctc
     7321 ccagctgaaa tattgccacc taagggaaga tggctggagc ctcagcgtcg attcaggtat
     7381 ctggtcccgg gccctctggc ttgctatttt ggccaagatc caggaacagg tgcctagccg
     7441 tcatccgcaa accataaagt tggaattgcc tgagagccaa cagcagagtt attttcttaa
     7501 agtctaccat tcggcttcgg gatggggcgc ggtgaaagat tggtttcggg cttcaaaggc
     7561 gctgcgtttt ctaaaacaag gcgaggcgct tgccaacgcg ggattcctcg ttccgccagc
     7621 agttgcggcg ggtgaaaaac gtcacttcgg catgttgaaa gcggcattcg tgctcacagc
     7681 cgctatcgag ggtgcgcctt tgacgaattt tctgaaagag aatgccggtc gcacggacgc
     7741 aggactctct ctaatcgaaa agagagcgac attgaaagct ttggcgcggc aaatccggcg
     7801 ctttcacgat ctcggattcg tccacggaga tttggtggct tcaaacatat tcgtctgccg
     7861 agatgcggcg atgaatttgc aattcgtttt catggataac gatcgcactc ggcgatattc
     7921 caaatggctg ccgcaatctt tgtggaagcg caatttagtg cagctcaacc gactacctct
     7981 ctcatccata tccttacaag atcgaatcag attctttcaa gaatactcta ccgcggaggg
     8041 cgctgcagat ctcaatcgcc ctttactacg gtggctggaa caaaagaccc gcgagcgccg
     8101 cagagagtgc gactcggtgg atgcg
//
LOCUS       DASUTG010000743         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1594993, whole genome shotgun sequence.
ACCESSION   DASUTG010000743 DASUTG010000000
VERSION     DASUTG010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594993"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1054)
                     /gene="galT"
                     /locus_tag="VF089_17160"
     CDS             complement(59..1054)
                     /gene="galT"
                     /locus_tag="VF089_17160"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX6175743.1"
                     /translation="MSELRKDPLIDRWVIIATERSKRPHADRTTTNLDRDEPCPFCAG
                     REGETPPEVLAYRDPSAPANTPGWKVRVVPNKYPALAGVGDISPSSDSAYVGLSGIGA
                     HEVVIESPKHCLRMAQLSERQVEEVLRAYRQRLIVLQNDERWKSILIYKNEGSAAGAT
                     LEHVHSQLLALPIVPREIDQEWRALIAHYDMRKRCLYCEMLDTERADGRRILFETPAF
                     IGFCPFASRFPFEICLMPKKHSPAFDTMASEELRELAFMLRQSLQRLARIVDAPFNYV
                     IHSAPLRELRRECYHWHLEILPRISKIAGFELASGYYINTVAPEIAARQLREVEL"
     gene            complement(1192..1800)
                     /locus_tag="VF089_17165"
     CDS             complement(1192..1800)
                     /locus_tag="VF089_17165"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX6175744.1"
                     /translation="MSYKVRDTYYKKAKQEGYRSRAAYKLLELQERFRIFKTGQLVVD
                     LGAAPGGWIQVAAKLVGPSGKVIGVDLQPIATFHQKNIIVVQTDITAAETEQRIMEYL
                     GRPADSVISDLAPKLTGIRDTDEARSSELNRTALEVAKRLLRPGGSFLIKSFVSEEMR
                     IFSTELERQFGSVQRTRPDASRKGSSEIYFFAKDYQPNLLAD"
     gene            complement(1873..2634)
                     /locus_tag="VF089_17170"
     CDS             complement(1873..2634)
                     /locus_tag="VF089_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6175745.1"
                     /translation="MKHPRMIAMTLAAWFALGMRPGFAAPDEYDDSQSHPLRIVAYLL
                     HPAAWLVEWAVFRPFHSLVSANEPMEAIFGHEPHPPVLAESQPNRDYGMPRRVPVKPA
                     PPPAPVAAAPDPVREVVKVVEVPKETIVYRDVEKVVEVEKFVFPGIAFQFDSAELTDW
                     GKGNVYLAAEKLKAKGDISVVIEGHTDEVGGAEYNKNLGLRRAQRVMTELAALGVDQA
                     RISTATQGEEKPLINQPTAWARAVNRRVEFEVRGK"
     gene            2782..>3161
                     /locus_tag="VF089_17175"
     CDS             2782..>3161
                     /locus_tag="VF089_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6175746.1"
                     /translation="MGLNKEKNPRVTYITIEHPPVNVLGIELLRELEQELTACAGDST
                     TDVVVLRGAGTRAFSAGVDIRDHTREKVPEMLEIVHGVIRKLLALPQVTIAAVQGVCF
                     GGGCEVATSCDFIVASDDSSFATP"
BASE COUNT          696 a          877 c          828 g          760 t
ORIGIN      
        1 tgattgacga ttgcttgcat cctctaaaat atgctgaaaa tcgaaaacga caagcttttt
       61 atagctcaac ctcccgcagt tgccgtgccg cgatttccgg cgccacggta ttgatatagt
      121 aaccggacgc caactcaaaa ccggcgatct tggaaattct tggtaagatc tcgaggtgcc
      181 agtggtagca ctcgcgcctc agttcccgga ggggcgccga atgaatcacg tagttaaacg
      241 gggcatcgac aatccgcgcc aggcgctgca aggattgccg cagcataaat gccagctctc
      301 gaagctcctc gcttgccatc gtgtcaaagg cgggcgagtg ttttttaggc ataagacaaa
      361 tttcaaacgg gaacctagac gcgaatgggc agaagccaat gaaagccggc gtttcgaaca
      421 gtatccggcg gccgtccgcg cgctcggtgt cgagcatctc gcagtagagg catctcttcc
      481 tcatatcgta gtgggcgatc agcgcgcgcc attcctgatc tatctcgcgc ggaactatgg
      541 gcaatgcaag tagttgtgaa tgaacatgct ccagggtcgc tcccgccgcg cttccttcgt
      601 tcttgtaaat aagaatcgat ttccagcgct catcgttctg tagaacgatg agacgttgtc
      661 gatatgcccg caagacttct tcaacctgtc tctcactcag ttgtgccatc ctcagacagt
      721 gtttcgggct ctcgataacg acctcgtgcg cgccgatccc gctcaggcca acgtacgcgg
      781 aatcacttga tggagagatg tctcccacgc cggccaaagc tggatatttg ttgggtacta
      841 ctctgacttt ccaaccgggc gtattggctg gagccgatgg atcacggtaa gcgagaactt
      901 caggtggggt ctccccttcc cggcccgcgc agaaaggaca tggctcgtct cggtctaagt
      961 ttgttgttgt acggtcagca tgaggacgct tgcttcgttc agtggcgata atgacccagc
     1021 ggtcgatgag cgggtccttt cttagctccg acattgccat ttgccgattt ggacctgggt
     1081 cacgctgaat gttaaaaggg cccgaccata catgctcagt agtgatcgct tgcatgacca
     1141 acggttctcc gggtgagagg agtgcgcgta cagctttgga tgcgcattcg gtcaatctgc
     1201 caggagattc ggctggtaat ctttagcgaa gaaataaatt tcagatgatc ctttgcggct
     1261 agcatcgggc cgggtacgct gaaccgagcc aaattgcctc tccagttccg tcgaaaagat
     1321 ccgcatctct tcactgacga agctcttgat aagaaagctg ccgcccggtc taagtaatct
     1381 ctttgcgact tccaaagcgg tgcggttcaa ttccgaggag cgcgcttcat ccgtatcgcg
     1441 gatgccggtc aacttaggcg cgaggtccga gatcacactg tccgcagggc ggccaaggta
     1501 ttccatgatt cgctgctctg tttccgccgc ggtgatgtcg gtttgcacga caatgatgtt
     1561 tttctgatga aacgtcgcga tcggctgaag atcaacgcca atgactttac cgctcgggcc
     1621 gaccagcttt gccgccactt gaatccagcc gccaggcgcc gcgcccagat cgacaactaa
     1681 ctggcccgtt ttgaagattc taaagcgctc ctgcagctca agcagcttgt aagccgcgcg
     1741 ggaacggtag ccttcttgtt tggccttttt atagtaggta tcgcggactt tgtaactcat
     1801 ctaagcgaaa tattcccgct gccaaaaaaa aggcccagct tgaggctggg cctttagacg
     1861 attaaaggtt acttacttcc ctctgacctc gaactctact cgtcgattta ccgctcgcgc
     1921 ccatgccgtc ggctggttga tcagaggctt ctcctctcct tgagttgctg tcgaaattct
     1981 cgcttgatca actcccaaag ccgctaactc tgtcataaca cgctgcgccc gtcgcagccc
     2041 gagatttttg ttgtattccg caccaccgac ctcatcggta tgtccctcga tgaccacgga
     2101 gatatcgcct ttcgccttaa gtttctccgc ggcaagatac acgttgcctt ttccccagtc
     2161 agtgagctcg gccgaatcga actgaaaagc aatccccgga aacacgaact tctccacttc
     2221 aacgactttc tcaacatctc tataaacgat cgtctccttt ggcacctcga cgactttcac
     2281 gacctcacgt acggggtccg gtgcggccgc tacaggggct ggaggaggcg ccggtttcac
     2341 cggtacccgt cgcggcatcc cgtaatctcg attgggctgt gactcagcca agaccggcgg
     2401 atgcggttca tgaccaaaga ttgcctccat cggctcattg gcgctgacga gagaatgaaa
     2461 aggtcgaaag actgcccatt ccactaacca ggctgcggga tgaagcaaat aggcgacgat
     2521 tctcaaagga tgcgactggc tatcatcgta ttcatcgggg gcggcaaaac ccggtcgcat
     2581 tcctaacgca aaccaggcgg caagagtcat ggctatcatt cgtggatgtt tcatggaagc
     2641 cctccttagg agctcgaagc cgtaagtcta ggttgcggtg ttttctttgt caaaccagtt
     2701 tttgtcagat atatcgctcg atcctgctcc ttacacgtgt accgactcgc cttttgtcag
     2761 attcgaaggt atagggatga catggggcta aacaaagaaa aaaatccacg ggtgacctac
     2821 ataaccattg agcatccgcc ggtaaacgtt ctcggtatcg aattgttgcg cgagctggag
     2881 caagaactaa ctgcatgcgc tggcgatagc acaaccgatg tcgtggttct acgaggcgcc
     2941 ggcacacgcg ccttttctgc cggcgtggat attcgcgatc acacccggga aaaggttccc
     3001 gagatgctcg agatcgttca cggcgtcatt cgcaagctgc tagctcttcc acaagtgacc
     3061 attgcagcgg ttcaaggggt gtgtttcgga ggtggttgcg aggtagcaac gtcgtgtgac
     3121 ttcattgtcg cctcggatga tagctcattt gccacccccg a
//
LOCUS       DASUTG010000744         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1150224, whole genome shotgun sequence.
ACCESSION   DASUTG010000744 DASUTG010000000
VERSION     DASUTG010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150224"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1670)
                     /locus_tag="VF089_17180"
     CDS             complement(234..1670)
                     /locus_tag="VF089_17180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175747.1"
                     /translation="MSKDLRSYMDELLERRPKDVVVVDREVDPQFEAAAIVEKFEREN
                     RFPLVFFKHIKGSKIPLVINLGATYERLALSLGSATVPQMVKDLAYREHNPLPVKEVS
                     AKEAPCKEIILKGDAVDLDLLPVLTHNEGDAGAYINAAALICKERGTGAVNVGLYRHQ
                     KQGKKQLGLMINPANHGNYVRAEYEDHNEPMEVALAIGHHPALNMAAVSKQAGVGSEM
                     EIAGAFLGEPLEVVKAETVDLMVPARCEIVIEGIVPPKKRQYEGPFGEWPHYYYKQGE
                     QPYIEVSAITMRRNPIYQSIHSAHFEHNIIGAAPRLGSLLRRIQEAVPSTVAVNLPIS
                     GAARAHCYISVRKRAEGDPKQAALAAFVTDPSIKHVIVVDDDVDVFNETQVLWALAMR
                     FQADRNLVIIPNIIGSHLNPTAYGHNRLEKGPMETKLIFDATKPLPPYDFPKDARAPE
                     QVLNKIDLARDTRPYEPGKDDRVMTGRN"
     gene            1988..>3154
                     /locus_tag="VF089_17185"
     CDS             1988..>3154
                     /locus_tag="VF089_17185"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175748.1"
                     /translation="MFEDLRGFLTHLESQKQLLRVKDEVDVKYEIAAGMRKTSDIEGP
                     ALLFENIRGYRGWRVLGGLFATRKLVALGLGVPQEQMLERYLTLEDERIPPEIISNAP
                     CKETRWTGDQVDLSKLPIVTHAGKDCGPYVTIGVQIGKNPDTGIRNLSIHRMLVLGKD
                     RLSLWAPADHHLGRMILMAEEKNKGLEVATAVGVDPAIVVGSQARVPFGIDEFHVAGG
                     LRGAAVKLTRCETIDVEVPAFAEVVIEGVTVPGERVADGPYGEYPGCYSEAKQAPVLK
                     VTAITMRQNPIWQTALTGMPVTENHTLIEYGNAAVVYREVRKVVPEVRGVNMTPGGTF
                     RHHVVVSIKKRAENEGRNVILALLSMGIGLKQVIVVDDDIDPFDPMQVDWAMATR"
BASE COUNT          699 a          851 c          879 g          725 t
ORIGIN      
        1 tttccgtttg cgagtgaacc gggaccgcga ggaccgtgaa gcagcttatg acaccaagaa
       61 ttccgaccca cagtcgattg tttggacctt gcatgtttct cctccctcga ataacccttt
      121 gtcagagaat aagctctcca cgctgaagtc ttctaaatct tcgcttcctg agatattccg
      181 cagcggacat ttaggctagg ccgtcggggc ttggcccaat tagtgaggac ctgttaattc
      241 ctgccggtca tcacgcggtc gtctttaccc ggctcatacg gacgggtgtc acgagctaaa
      301 tcgatcttgt tgagcacttg ctccggcgct cgggcgtcct tggggaagtc atatggtggc
      361 agcggtttcg tcgcatcaaa aatcagcttt gtttccatcg gacctttttc caggcggttg
      421 tgaccatagg cagtagggtt taaatgggag ccaatgatat tcgggatgat cacgagatta
      481 cggtcggctt ggaaacgcat cgccagcgcc cagagcacct gcgtctcatt gaaaacgtct
      541 acgtcatcat caacgacgat gacgtgtttt atactgggat cggtaacgaa agccgccagc
      601 gcggcctgct tgggatcacc ttcggcgcgt tttctcaccg agatatagca gtgtgcgcgc
      661 gccgcgccgg atatcggcag attgaccgcg acggtgctag gcacggcttc ttgaatccgg
      721 cgcagcaagc tgccgaggcg cggtgccgcg ccgatgatgt tgtgctcgaa atgggcgctg
      781 tgaatgctct gataaatcgg attgcgccgc attgtgatgg cgcttacttc gatatagggc
      841 tgctcgcctt gcttgtaata gtaatgcggc cattcgccga aaggtccttc gtactgccgt
      901 ttcttcggtg gcacgatgcc ttcgataacg atttcgcagc gcgccggcac cattagatcg
      961 acggtctcgg ccttgaccac ttccaatggt tcaccgagaa aggcgcccgc gatctccatt
     1021 tcggagccga cgccggcctg cttactgacc gccgccatat ttaatgccgg gtgatggccg
     1081 atggccaggg caacttccat gggctcattg tgatcctcgt actcggcgcg aacatagttg
     1141 ccgtggttgg ccggattgat catcaaaccc aattgtttct ttccttgctt ttgatgacga
     1201 taaagaccga cgtttaccgc gccagtgccg cgctccttgc agatcaatgc cgccgcattg
     1261 atgtaagctc ccgcgtcccc ctcgttgtgc gtcagcaccg gcaataggtc gagatcaact
     1321 gcatcgcctt tgagaatgat ttctttgcac ggcgcctcct tcgctgaaac ttctttcacc
     1381 ggcaacgggt tgtgctctcg ataggcgaga tccttaacca tctgcggcac agtcgccgaa
     1441 cccagcgata gtgccaggcg ttcgtaagtc gcacccagat tgatcaccag cgggattttg
     1501 gaacctttga tgtgcttaaa aaacaccagg gggaacctgt tctcacgttc gaatttctcg
     1561 actatagcag cggcttcaaa ctgcggatcg acttcacggt cgacgacgac cacatctttg
     1621 ggacgccttt ccagtaattc gtccatgtag cttcgcaaat ccttagacat cgactatgac
     1681 ctcctaatgt tccgataatt gctggtggta gtggaaccat gcagtacttg ccttttagac
     1741 gcacaggtat attgcgaaat cgacccctgc tgtcaagcaa cgattgccaa actcctctcc
     1801 ctggctcggt cgagcaatgc acactgggcg ggaagctatc gatgatgaat gccaggagcc
     1861 ggtctgtgaa attcttctca ctcaagcagg acccctaatc cagttgaagt gacgggcaaa
     1921 acgctgataa aggtcaaacc gatggttgat cagattaatt cttgcgagcg atagcgagga
     1981 actacgcatg ttcgaagact taagaggttt cctgacgcat ctggaaagtc aaaaacaact
     2041 cctccgggtc aaagacgagg ttgacgtcaa atacgaaatc gccgcaggga tgcgcaaaac
     2101 ctccgatatt gaaggcccgg cgcttctgtt tgaaaatatc agaggctatc gcggctggcg
     2161 cgtcttgggc ggactcttcg cgacgcgaaa attggtggcc ctcggcctcg gtgtgccgca
     2221 agagcagatg ctcgagcgtt acctgactct cgaggacgag cgcattccac ccgaaattat
     2281 ttcgaacgca ccctgtaagg aaacaagatg gaccggcgat caagtcgatt tatccaagct
     2341 gccgattgtc acgcatgccg gtaaagattg cggaccctat gttacgatcg gcgtgcagat
     2401 cggtaaaaac cccgacaccg gcatccgcaa tctttcgatt caccgcatgc tcgtgctggg
     2461 aaaagatcgc ctgtctctgt gggcgccggc cgatcatcac cttggcagga tgatcttgat
     2521 ggccgaggaa aagaacaaag gcttagaagt tgccaccgcg gtcggcgtcg acccagccat
     2581 cgtcgtcggt tcgcaggcac gggtaccgtt cggcatcgac gagtttcatg tcgccggcgg
     2641 tttgcgcggc gctgcggtga agctaaccag atgcgaaacg attgacgtcg aggtgccggc
     2701 gttcgccgaa gtcgtgatcg aaggcgtgac agttccaggc gaaagggtag ccgatggacc
     2761 gtacggagaa tatcccggct gttacagtga agccaagcaa gcgcccgtgc tcaaagtgac
     2821 ggccattacg atgcgtcaga atccgatctg gcagacggcg ctaaccggca tgccggtgac
     2881 ggaaaaccac acgttgatcg agtacggcaa tgcggcggta gtttatcgag aggtgagaaa
     2941 ggttgtgccc gaagtacgcg gcgttaacat gaccccggga gggacctttc ggcaccatgt
     3001 cgtggtgtcc attaaaaaac gggccgagaa cgaagggcgc aatgtcatac tggcgcttct
     3061 ttcaatgggc atcggtctca agcaggtgat cgttgtcgat gacgatatcg atccctttga
     3121 cccgatgcag gtagattggg cgatggcgac acgg
//
LOCUS       DASUTG010000745         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1595346, whole genome shotgun sequence.
ACCESSION   DASUTG010000745 DASUTG010000000
VERSION     DASUTG010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595346"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VF089_17190"
     CDS             <1..1011
                     /locus_tag="VF089_17190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175749.1"
                     /translation="EILKLRTKVENNEIIAPTIFTASPLLDGDPPIWSGSGTIVLTNP
                     KDAERVVIDQKRAGYDFIKIYNRLTPDVYLAVLKAAKENNIAVVGHIPRQVGAEEALR
                     AGQTMIAHGEEFFFTYFGGPADKLNQAENRVKPDEGKIPAIAQQTHKAGTAVTPTLSF
                     IEATKRQLEDFDAVISDPEARFLTPDVLSMWKSSNPNNRRNLEQFKEREKLKYPLVKA
                     LTKGFHDAGVLLLLGTDASAPGLFPGKSAHTEIRELVGAGLTPFQALSTGTRNAGDFI
                     RNNVKSAVPFGTIRVGQKADLILLNKNPLQDINSISSINGVMTRGRWLPKAELQQMRR
                     EN"
     gene            1039..2424
                     /locus_tag="VF089_17195"
     CDS             1039..2424
                     /locus_tag="VF089_17195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175750.1"
                     /translation="MKNPKVALTLLVMILCLDSVALGQRKVEVHVKDISIFDPHTGRM
                     IPHRDIVVSDTRIVDVLPAGKYTGSAKTVINGSGKYAIPGLFDNHVHMARMTEVTAGL
                     FLVYGITSVRDMGTEPARIVDWRKRIAYGKFYGPRIVQACGPMLESRGEERKDHWVVK
                     TPEDARAVVARIAASGMDCVKLRTFADEKTYLAIAAAAAEYKLMLTGHAPEQLDPELA
                     LKAGQRTFEHAFYPYPLSKLTDEKKQALFSAFKASRAAVVPTLIAWKPFTQPTSELES
                     MLNTYREGKDFELSPELLDHWERTIQVHRTQKRGSKGWRDAVTTASSDIGEMHKAGVT
                     VLPGSDTGAPFVVPGLALHEELYLLVHAAGMTPAEVLSAATIGSAAFYGREKELGSIE
                     PGKIADFVILNKDPLQDITYTRDIDAVVFRGEALTLAHLNIFRGKTKLGHKARLQPGF
                     ETAKLVRYVSR"
     gene            2593..3141
                     /locus_tag="VF089_17200"
     CDS             2593..3141
                     /locus_tag="VF089_17200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6175751.1"
                     /translation="MPSEESSGTLDQILVKRFLVYGDEKSFRELYRRYTPALYSLALR
                     LVNGSAPDAQDAIQDTWIRACKSLARFEWRSSLRTWLTGILINRVREFTREYQSRHEE
                     NLTEESKLADSTPTEQRIDLEQAIARLPNGYRHVLILHDAEGYTHEEIGVLLDVSVGT
                     SKSQLHHARQAVRALLNGVNIK"
     gene            3138..>3591
                     /locus_tag="VF089_17205"
     CDS             3138..>3591
                     /locus_tag="VF089_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175752.1"
                     /translation="MNEDLDQELTPQERRAFDNLRKASPPPFLEERVVDALKEMNLIR
                     SSAAARWPRFAKPGIAVAAALLLIALGAMMGSKWLSAPQPSPQAEFMLVLRSAPRESS
                     ATSSDEVMRRVKEYSRWASEMRKKGMMVGGEKLKDEARVLTVTDGRVSV"
BASE COUNT          930 a          908 c          958 g          795 t
ORIGIN      
        1 gagatcctaa aactgagaac caaagtcgaa aataatgaaa ttatcgctcc tactatattt
       61 acggccagcc cacttcttga tggcgatccg ccgatttgga gcggaagcgg gaccattgtc
      121 ttaaccaatc ctaaagatgc cgagcgagtt gtaattgatc agaaaagagc cggttatgat
      181 ttcataaaga tatataacag actaactccc gacgtttatc ttgcggttct caaagccgcg
      241 aaagaaaaca atatcgctgt ggtgggtcac attccccgcc aggtcggcgc cgaagaagcc
      301 ctgagagccg gccagacaat gattgcccat ggtgaagagt tctttttcac ttatttcgga
      361 ggacccgccg acaaactaaa ccaggctgaa aatcgagtta aacccgacga aggcaagatt
      421 cctgcaatcg cacagcaaac gcataaagcc gggactgccg tcacgccgac tctgtctttt
      481 atcgaagcca cgaaaaggca gcttgaggat tttgatgcag ttatttcaga ccctgaagcc
      541 aggtttttga cgcctgatgt tttgagtatg tggaagtcga gtaatcccaa caatcgtcgc
      601 aatctggagc aattcaaaga aagagaaaag ttgaagtatc cattggttaa ggcactcacc
      661 aagggtttcc atgatgccgg agtgttgctg cttttaggaa cggacgcttc tgccccggga
      721 cttttccccg gcaagtcagc gcacactgaa attcgggagc tggtcggggc agggttaaca
      781 ccttttcagg ccctttcgac aggcacgcgc aacgccggag acttcattcg taacaacgta
      841 aaatcggctg tgcctttcgg gacaataaga gtcggacaaa aggccgattt gattctgctc
      901 aacaagaatc ctttgcagga tattaattca atttcgagca tcaacggtgt gatgactcgg
      961 ggacggtggc tgccaaaggc ggaacttcaa caaatgcgaa gagaaaacta agacgggcgc
     1021 cccatcgata aaacgactat gaagaatcca aaagttgctt taacactatt ggtaatgatc
     1081 ctttgcctgg attcggtggc cctcggtcag cgtaaggttg aagtacatgt aaaggacata
     1141 tccattttcg atccgcatac cggcagaatg attccccatc gcgacatcgt cgtttcagat
     1201 acgcggatag tagatgttct gcctgcggga aagtacaccg ggagcgcgaa gactgtcata
     1261 aacggatccg gcaaatacgc tatccccggc ctcttcgata atcacgtaca catggcgcgt
     1321 atgaccgagg tgacggccgg tctttttctt gtctacggca taacgtctgt gagggatatg
     1381 ggaacggagc ccgcaaggat cgttgactgg cgaaagcgaa tagcgtacgg caagttttac
     1441 gggccgcgta tcgtccaggc ttgcgggccc atgctcgagt caaggggaga agaacgaaag
     1501 gaccactggg tagttaaaac ccccgaagac gcgcgggcgg tggttgctcg tatagcggcc
     1561 tccggtatgg actgtgtaaa gttaagaacc ttcgcggatg agaaaactta ccttgctatt
     1621 gccgctgcag cagctgaata caagctcatg ctcacaggtc acgcacccga gcaactcgat
     1681 ccggaacttg cgttgaaagc tggccagcgc acattcgaac atgcgtttta tccgtatccg
     1741 ctgtcaaaac tgacggatga aaagaagcaa gctctatttt ctgcattcaa ggcgtcgcgg
     1801 gctgcggttg ttccgacctt gattgcgtgg aagcccttta cgcagccgac ctcggaactc
     1861 gagagcatgt tgaacactta ccgggaggga aaggatttcg aactctcgcc ggaattactc
     1921 gatcactggg aacgaacgat acaggtccat agaacccaaa agcgcggttc caaaggttgg
     1981 cgggatgccg tcaccacagc atcatcggac attggcgaaa tgcataaggc cggcgtgacg
     2041 gtcttgcctg gaagcgacac gggcgcccct ttcgttgtac cgggtttggc tctccacgag
     2101 gaactatatc ttttggtcca tgcggccggg atgacccccg cggaagttct gagtgcggcc
     2161 acgatcggat cagctgcgtt ctacggtcga gagaaggaac tcggctcgat tgagccaggc
     2221 aagatcgccg atttcgttat cctgaacaag gatccgcttc aagacattac atatactcgt
     2281 gatatcgacg cggtcgtatt caggggcgaa gcgctgacac ttgctcattt aaacattttt
     2341 cgaggtaaaa caaagctggg ccataaggcc agattacagc cagggtttga aaccgcgaag
     2401 ctggtacggt acgtttctcg atgatgcgac atcgttcgct atcgacaagc taataaagca
     2461 cgtcgacact tcctaagctc tggcggccaa aatttcgaca aacttcagtt tctcgtttga
     2521 gatcgaccag ctaaaccact tcggacaaat agcggtacaa ccctcgacca tcatttcctc
     2581 actcatcatt agatgccaag tgaagagtcc agcggaacac ttgatcaaat cctggtaaag
     2641 agatttctag tctatggaga tgagaaatca ttcagggagt tgtatcgccg ttacactcct
     2701 gccctgtatt ccttagcctt gcgtctggta aacggctctg ccccggatgc tcaggacgcg
     2761 attcaggaca cctggattcg tgcctgtaaa tcgctggctc ggtttgaatg gcgatccagc
     2821 ctacgcacgt ggctgacggg cattttgatc aatcgcgtca gggagttcac tcgcgaatat
     2881 caatcgcgcc acgaagagaa tcttaccgag gaatccaaac tcgcggacag cactccaacg
     2941 gaacaacgga ttgacctgga gcaggcgatc gcccgccttc ccaacggcta ccggcacgta
     3001 ctgattctgc acgacgccga aggctacacc cacgaagaaa taggtgttct cctggacgtc
     3061 agcgtgggca cttcgaaaag tcagcttcat catgctcgcc aggcggtcag ggccttgctg
     3121 aatggagtaa atattaaatg aacgaagacc tcgatcaaga actaacaccc caggagcgac
     3181 gggcctttga caacctcaga aaagcttcgc ctccgccttt tctcgaagag cgcgttgtcg
     3241 atgcgctcaa ggagatgaat ttgatccgtt cttccgcagc tgcaaggtgg ccgagatttg
     3301 caaagccggg cattgccgtc gccgcggcgc tgctgctcat cgcgttgggt gcaatgatgg
     3361 gctcgaagtg gttatcggcc cctcaacctt cgccacaggc tgagttcatg ctggtgctgc
     3421 gaagtgcccc gcgagaatca tcggcgacat cttcggatga ggtcatgaga agggtgaagg
     3481 aatacagccg ctgggccagc gagatgcgta aaaagggaat gatggtaggc ggtgagaagc
     3541 tgaaagacga agcacgcgtc ttaacggtta ctgatggacg cgtgtctgta a
//
LOCUS       DASUTG010000746         9247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_410574, whole genome shotgun sequence.
ACCESSION   DASUTG010000746 DASUTG010000000
VERSION     DASUTG010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410574"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF089_17210"
     CDS             complement(<1..442)
                     /locus_tag="VF089_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175753.1"
                     /translation="MNQAPEILRRKFLQLFGWERRKTRHEILTTALCYSLFAALLLLP
                     IHFLLPASFLRWFMPLPIFFALVAYLFVRRRWRREDSARVLVDLDKALHSDERAITAW
                     ELIETKADRPAALLVLKQAAECLKTLDPSALFSRSRRWQEYAVIP"
     gene            complement(439..1185)
                     /locus_tag="VF089_17215"
     CDS             complement(439..1185)
                     /locus_tag="VF089_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175754.1"
                     /translation="MKSNPVLRESIDIFFVEGHGFAVYFYILVILAPVEFLSLYLPSL
                     DAQMWSGSASLFKVAAVTTLLLAIYFAMRVANQEFASWRFRTLKRWHRENDQPIAAIA
                     QAQLVFLSLHVALSLLLCAPLLIWAAAIARTPFSMVALTFGLLFFYAWGYATWGLFAL
                     TLWERRAESRQVFIRSFFFTLTILSLLVYLPLNPIAFLLAILSRQEFPPLSIAGYKWP
                     AGILHFSVHFLFVGLALLAHQWALRKELRQ"
     gene            complement(1182..3212)
                     /locus_tag="VF089_17220"
     CDS             complement(1182..3212)
                     /locus_tag="VF089_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175755.1"
                     /translation="MSFSVRKAAQGFSRGQLVARRQQAVLALPKFWLESFQIGSRAAL
                     IGFFLSGLLFSSLSYAEISIEARVGFHGVFQLGRPFPLEVEVTNSGRPADGFLEIQVW
                     KSGAARSGVPYPLHHRRDLFVSGQSRRTIQLTVDPDFISRPLLISFVSPAGSASRELD
                     LRRYFSPSPVMLLVSDASSLSISLGSAMPNRLVAVSLSELPSDPRALLGVSHLILYDQ
                     SLRELSRSQLFALESWLSAGGRMVIIGSLNYALYQETSLSRFLPVRITGSERISFAPQ
                     SVQGEPTTPIPGVWAQVSSLLDGKVLLESGGIPLLVERSRGKGRITYFALDLGRPPLA
                     QWDGLPMLLRQLVRPATDDALSPRTRWDDTIFSQIIVSPSFISTYVPTRSLLLAIIGY
                     FAALGILAWLWQKSRLPARRIIMCLAIVVAMAAVAGYSLFSRGGNVPDGVLLSSTLLD
                     SVADGYVEAQLSMAMFSTQIRQYNLQLARGWLDMAPVSMPAKDRPQEALLLQDGSGSS
                     RFRLPLREWDYRLFRMRFIDRFAMRAEFEIQGDKLLMKIDNQTGKDLVDCWLVLPGAR
                     HRLGEITRGSRWSKVFSLNSGEAPEANQSSRSDALNLRDLSFNDKTRDILFHSSFFPR
                     DGEASRWNTGAAIFFGWVKDSERRVSVDDPRIHAHDYTLFRSIISLPAPEDE"
     gene            complement(3252..3740)
                     /gene="greA"
                     /locus_tag="VF089_17225"
     CDS             complement(3252..3740)
                     /gene="greA"
                     /locus_tag="VF089_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010920688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6175756.1"
                     /translation="MATQDERVPMTTQGYQSLMDELKRLKSVERPKNVREIEEARGHG
                     DLSENAEFHAAKERQSLLDIQIREIEDRLARAQVIDVSTLSGDRVVFGATVALADGDT
                     GDKVVYQIVGDHEAEPKNGKISISSPVARALIGKSEGDEVQVQTPTGVRSFEILSVEY
                     ID"
     gene            complement(3933..4916)
                     /locus_tag="VF089_17230"
     CDS             complement(3933..4916)
                     /locus_tag="VF089_17230"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX6175757.1"
                     /translation="MTMSLLEIRDLKKHFPVGQALFSRNKDVLKAVDGVNLSVEQGET
                     LGLVGESGCGKSTLGRAILRLIEPTSGEVYFEGKNLLAMSQRELRDMRRQMQIIFQDP
                     YASLNPRMRVGDIVGEGLEIHKIAKGRAKRDRVIELLRQVGLREDHYGRYPHEFSGGQ
                     RQRIGIARALGVGPKFIVCDEPVSSLDVSIQAQIINLLQELQEKMHLTYLFISHDLRV
                     VEHISHRVAIMYLGKVVEIAQSDTIYRDAKHPYTRALLSSVPIPEVDGKKERVLLQGD
                     VPSPVNPPSGCAFHPRCAYRAGICDQMEPPLDFDAASHGVACHVFGPVKRA"
     gene            complement(4960..5985)
                     /locus_tag="VF089_17235"
     CDS             complement(4960..5985)
                     /locus_tag="VF089_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175758.1"
                     /translation="MAELIKVKDLRTSFFTPEGEVRAIDGISFEIGEGKTLGLVGESG
                     CGKSVTSLSIMRLIASPPGKIVGGEILYRGRDLLKLKNEEMRKIRGNEISMIFQEPMT
                     SLNPVFTVGNQIGEAIRLHQGLGKKETRQKTIDMLRLVKIADPESRVDAYPHQLSGGM
                     RQRIMIAMALSCNPSLLIADEPTTALDVTIQAQILDLMKELQQRLGMALLLITHDLGV
                     VAEQADEVAIMYGGKIVERATARAIFTRPFHPYTVGLLNSLPGTGRLKKKRLDAIPGV
                     VPNPLHLPSGCRFRDRCPRAADLCAQIEPELLEKEPGHTVACHFPHTEWKQKLEREIE
                     MQTQGSA"
     gene            complement(6212..6337)
                     /locus_tag="VF089_17240"
     CDS             complement(6212..6337)
                     /locus_tag="VF089_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175759.1"
                     /translation="MKIRNPALDKVKVGDMIDDRILRQLDESGIIDQLAKTYPAK"
     gene            complement(6324..6548)
                     /locus_tag="VF089_17245"
     CDS             complement(6324..6548)
                     /locus_tag="VF089_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175760.1"
                     /translation="MTPHTLPTTVLAAVVGGADLKTIGGLTRRLRYAAPNIKAPKDPR
                     RTTTGYYQWCGTKTFPVVEGLRNSAAHEDS"
     gene            complement(6580..7350)
                     /locus_tag="VF089_17250"
     CDS             complement(6580..7350)
                     /locus_tag="VF089_17250"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6175761.1"
                     /translation="MKLQNQVAVVTGAGRNIGKAIAKLFANEGARIAVIETHEGRGQA
                     VVQELENSGHEALLVLCDVANSSDVQQMLQKVVARFGGIDILVNNAALTDHADIFTST
                     EDEFDKVIAVSLKGPYLVTKHVAAQMAKQGRGGKIVNFGSTSGMLGRTDGIAYAAAKG
                     GVINLTRAMAVQLSPYKIRVNCVVPNRSGSPVGYDDDVAGSRTFQNLAGRLGTAEDQA
                     KAALFLVSDDSDFIYGHPLVVDGGVVATANFPSEQFVK"
     gene            complement(7416..7964)
                     /locus_tag="VF089_17255"
     CDS             complement(7416..7964)
                     /locus_tag="VF089_17255"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6175762.1"
                     /translation="MARIAIIEKKEDLQPEHQPVYQAIAQSRGVVGGPFLALLHSPEL
                     AGRTAHLGSYVRFESSLDPKVIELAALVSARELQCKHEWAAHVNHAQKAGIPLDTIRA
                     VHQSKDAEHFTFEDAQIVSFVRELLRAHRVTETTFQALYGRLGERGLVEITATVGYYA
                     MLACTLNTFDIATATAPDEFKI"
     gene            complement(8172..>9247)
                     /locus_tag="VF089_17260"
     CDS             complement(8172..>9247)
                     /locus_tag="VF089_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175763.1"
                     /translation="TPVSTTNDASSPETADLAGGSAVSAEGLWTGVWKQFKRNPIARI
                     SFYVVCFLFLVALFADFLANNKPYYLSYREQTYFPIFRSYLVRAGLGQWPKELLNVDF
                     KKLEGAHGIFPPVPYLSTDIDLTAPFENPSPKHWLGTDRLGRDVLAGMIHGSRVSLSI
                     GFVAVGIAVLIGITLGAIAGYFGSWIDLVISRLFEIMLAIPTFFLLITVAALLPPSIY
                     LTMLIIGATSWVGIARFTRNEFLKIRSLDYVTSALALGVSNRKVMFKHILPNALAPVI
                     VSVVLGVAGAILVESSLSFLGIGVPAELVTWGSILHEASSATFAWWLAVFPGLAIFIT
                     VLAYYLVGEGLREVLDPRLRGLR"
BASE COUNT         2235 a         2636 c         2484 g         1892 t
ORIGIN      
        1 gtggaatgac cgcatactcc tgccagcgcc gggaccggct gaacagcgcg ctcggatcga
       61 gtgtcttcag acactccgct gcctgcttga ggaccagaag cgctgcgggt cgatctgctt
      121 tggtctcgat cagctcccaa gcggttatcg cccgctcatc cgaatgcagc gctttgtcga
      181 gatcgaccag cacgcgcgcc gaatcttccc ggcgccaacg gcggcggacg aagagatagg
      241 ccaccagagc aaagaaaatc ggcagcggca tgaaccaacg gagaaagctt gccggtagca
      301 ggaagtggat tggcagtaac aacaatgccg cgaacagtga atagcacagc gcggtggtca
      361 ggatctcatg cctcgttttg cgcctctccc aaccaaacaa ttgtagaaat tttcgacgga
      421 ggatttccgg cgcctggttc attggcgcaa ctccttgcgc agcgcccact ggtgagcgag
      481 caaagctaag ccgacgaaca gaaaatgaac ggaaaaatga agtatgcccg cgggccattt
      541 gtatccggcg atactcagcg gcggaaattc ctgccgcgat agaatcgcca ggagaaacgc
      601 aatggggttg agcggcaagt agaccaggag tgagagaatc gtcaaagtaa agaaaaatga
      661 gcgaatgaac acttggcgac tttcggcacg gcgctcccac aacgttaatg cgaacagccc
      721 ccaggtggcg tagccccagg catagaaaaa caacagccca aaggtgagcg ccaccatcga
      781 aaacggtgtc cgcgctatgg cggctgccca gatcaacaaa ggcgcgcaca ggagcagaga
      841 aagcgcgacg tgtaacgaca aaaacacaag ctgcgcctga gcgatcgcgg cgatgggctg
      901 gtcgttttca cgatgccagc gtttaagtgt ccgaaatctc caggaagcaa actcctgatt
      961 ggccacccgc atcgcgaaat aaatcgcgag caacaatgtc gtcaccgccg cgaccttgaa
     1021 gagactcgcc gaaccgctcc acatctgcgc atcgagtgag ggcaagtaaa gcgagagaaa
     1081 ctcgaccggc gcaaggatca ccaagatgta aaaataaact gcgaacccat ggccttcgac
     1141 gaaaaaaatg tcgatgcttt cgcgcaaaac cggattactc ttcattcatc ctccggcgca
     1201 ggcagtgaaa tgattgaccg gaacaatgtg tagtcgtggg cgtggatgcg cgggtcatcg
     1261 acgctgaccc gccgttccga gtcttttacc cagccgaaaa agatcgcggc gccagtgttc
     1321 cagcgcgacg cctcaccgtc gcgcggaaag aaagaagaat gaaacaagat gtcacgtgtt
     1381 ttatcgttaa aagacaaatc acgcaggttg agcgcatcgc tgcggcttga ttgatttgcc
     1441 tcaggcgcct ctccagaatt caaagaaaaa accttgctcc atcgtgaacc tcgggtgatc
     1501 tcgcccaagc gatggcgcgc gccgggcaac accaaccagc agtccaccag atctttaccg
     1561 gtttggttgt cgatcttcat cagcagctta tcgccttgaa tttcaaattc cgcgcgcatc
     1621 gcaaagcggt caataaagcg catgcgaaaa agcctataat cccactcgcg cagcggtaag
     1681 cggaagcgac tggaaccgct gccatcctgc aacaacaacg cttcttgcgg ccgatctttg
     1741 gccggcatcg acaccggcgc catatcgagc caaccacgag ccaattgaag gttgtactgg
     1801 cggatttgag ttgaaaacat cgccatgctc agttgagctt caacgtagcc gtcggcaaca
     1861 ctatcgagca aagtggaaga gagaagcaca ccgtcaggga cattaccacc gcggctaaac
     1921 agtgaatacc ccgccacggc ggccattgcg acgactatcg ccaggcacat gatgattctc
     1981 cgcgccggca accggctctt ctgccatagc caggctaata taccgagagc cgcgaaatag
     2041 ccgattatgg ctagcaacaa ggaacgggtt ggcacataag tcgagataaa tgacgggcta
     2101 acgatgatct gagaaaagat ggtatcgtcc caacgggtac gcggcgacag cgcatcgtcc
     2161 gtggcgggac ggacaagttg gcgcagaagc atgggcaaac catcccattg agccagcgga
     2221 ggtcgtccaa gatcgagggc aaaataggtg atccggccct tgccccggct ccgctcaacc
     2281 agaagcggaa ttcctcctga ctccaggaga actttaccat ccagcaagct tgaaacctgc
     2341 gcccagacac ccgggatcgg ggtcgttggt tcaccttgga cgctttgcgg agcgaaggag
     2401 attcgctcgc tgccggtgat gcgcacaggc aaaaagcggc tgagtgaggt ctcctgatat
     2461 aacgcatagt tgaggctgcc tatgatgacc atcctaccgc ccgcactgag ccaactttcc
     2521 aaggcaaaca gctgcgagcg cgacagctcg cgcaacgact ggtcatagag aatcaaatgt
     2581 gaaaccccga gcagagctcg cggatctgac ggtagctcgc tcaatgaaac agccaccaaa
     2641 cgattgggca ttgccgagcc cagagagatc gagagagaac tggcatcgct caccagcagc
     2701 atgaccggcg acggtgagaa gtatcgtcgc agatcgagct cgcgcgacgc gctcccggcc
     2761 gggctgacaa aagagatcaa cagcggccgg ctgatgaaat cgggatctac agtaagttgg
     2821 atggttctcc gcgactggcc ggaaacgaaa agatccctgc ggtgatgaag cggataagga
     2881 acgccgctcc tagccgcgcc gcttttccaa acctgaattt ccaaaaagcc gtcagcggga
     2941 cggcccgagt tagtgacctc gacctccagc ggaaacggac gtcccagctg aaatacgccg
     3001 tgaaagccga cccgtgcttc aatcgatatt tcggcgtaac tgagagatga gaaaagaaga
     3061 ccggatagga agaagcctat gagagcggct cgtgaaccga tttgaaatga ttcgagccaa
     3121 aacttaggca gtgccaaaac cgcctgttga cgcctagcta ctagttggcc gcggctaaag
     3181 ccttgagctg cctttcgcac tgaaaaggac atgatccctt tccagaaagg cccaccccat
     3241 tgcagcagag atcaatcgat gtactcgaca cttaagatct cgaatgaacg cacgcccgta
     3301 ggggtctgga cctgaacctc gtccccctcg ctcttgccga tcaaagcacg ggcaacggga
     3361 gaactgatag aaatttttcc attcttcggc tccgcctcat gatcaccgac gatctgatat
     3421 accaccttgt cgccggtgtc gccatcggcg agagcgaccg tcgccccgaa caccacccta
     3481 tcgccggaca gtgttgaaac atcgatgacc tgagcgcgcg ccaggcggtc ctcgatctcg
     3541 cgaatctgaa tatccagcag ggattggcgt tccttggccg catgaaactc ggcgttttcg
     3601 gaaagatcgc catgacccct cgcctcttcg atttcccgaa cgtttttagg acgctcgaca
     3661 ctctttaggc gttttagttc gtccatcaaa gactgatatc cctgagtagt catcgggaca
     3721 cgctcatcct gtgttgccat agattcaact cctatacaaa acaaaacctc tcgggaatga
     3781 ctcctgagag gctctcgctc gatcgcttca cctagtcgcg cgtgaagcgc cactctctac
     3841 ggatggtagc atcctcggcg gttaattcaa ctctcgggaa gcacttctaa gggtccatcg
     3901 acaagctggc cgaaggtttg acggaagcag gatcaggcgc gttttacagg gccaaaaaca
     3961 tggcaggcga cgccgtgaga agcggcatcg aaatccaatg gcggctccat ctgatcacag
     4021 atccctgcgc gataggcgca acgcggatga aaagcacacc ctgacggagg atttaccgga
     4081 ctgggcacat caccttgcaa cagcacccgt tcctttttcc cgtccacttc ggggatcggc
     4141 actgatgata gcaacgctcg ggtataagga tgcttggcat cgcgatagat cgtatcgctc
     4201 tgggcaattt ccacgacttt gcccaaatac attatggcca cccggtggct gatatgctcg
     4261 accaccctca ggtcatgcga gataaacagg taggtcaaat gcatcttctc ttgcaactcc
     4321 tgcaagagat tgatgatctg cgcctgaatc gaaacgtcca gcgaagatac cggttcatcg
     4381 cagacgataa acttgggacc gacgccgagc gcccgcgcaa tgccgatgcg ctgccgttga
     4441 ccaccgctga actcatgcgg gtaacggcca taatggtcct cgcgcaaacc gacttgacga
     4501 agcagttcga tgacccggtc tcgtttggcc ctgcccttgg cgattttgtg aatttccagc
     4561 ccttcgccga cgatatcgcc gacgcgcatt cgcgggttga gggatgcata cggatcttgg
     4621 aaaatgattt gcatctgccg ccgcatatcg cgcagctcgc gctgcgacat ggccaataaa
     4681 ttcttaccct cgaagtaaac ttcaccgctg gtgggttcaa tcagccgcaa gatggcccgg
     4741 cccaaagttg acttgccgca accggactcc ccaaccagac ccaacgtctc gccttgttcg
     4801 acgctaagat taacgccgtc gacagctttg agcacgtcct tattgcgcga aaatagcgct
     4861 tggccgactg ggaaatgctt tttaagatct cggatttcta acaggctcat ggtcatacca
     4921 gaattcggat aggccggcga ggaatcgcca ggttgccttt catgctgatc cctgtgtctg
     4981 catttctatc tcccgctcaa gcttttgttt ccactcggtg tgcggaaaat ggcaggcaac
     5041 ggtatggccc ggttctttct caaggagctc cggttcgatc tgggcacata gatctgccgc
     5101 ccttggacag cgatcccgga aacggcagcc actcggcaga tgcaaaggat tcggcaccac
     5161 cccggggatc gcgtccaggc gctttttctt cagacggccg gtgccgggga gcgaatttag
     5221 tagccccacg gtatacggat gaaagggtct ggtgaatatc gcccgggcag tggcgcgctc
     5281 gacgatcttg ccgccataca taatggcaac ttcatcggct tgctccgcca ccacgccgag
     5341 atcgtgggtg atcagtaaca gcgccatgcc cagtctttgt tgcagctctt tcatcaaatc
     5401 cagaatctgc gcttgaatcg tgacgtcaag cgccgtggtc ggctcgtccg cgattaacaa
     5461 gcttgggtta caggacagcg ccattgcgat cataattctc tggcgcatcc cgccgcttaa
     5521 ttggtgcgga taggcatcga cccgagactc gggatcagcg atcttgacca gccgcagcat
     5581 gtcgatggtt ttttgccggg tttctttttt tccgagcccc tgatgcagcc ggatcgcttc
     5641 gccgatctga ttgccgacag tgaacaccgg attgagcgag gtcatgggtt cctggaaaat
     5701 catcgagatt tcgttgccgc ggattttgcg catctcctca ttcttcagct tgagaagatc
     5761 ccggccccga taaagaatct ctccaccgac gatttttccc ggcggtgagg cgatcagtcg
     5821 catgatcgaa agcgaggtca cactcttgcc gcagccggac tctccaacca agcccagggt
     5881 cttgccttca ccaatttcga aactgatgcc gtcaatcgcg cggacttcac cttccggagt
     5941 gaaaaaagac gttcgcagat ctttaacttt aattagctcg gccatctatt cggtctcgcg
     6001 ccggcgaatg aagcaacaaa acgtgatttg tcgaaataac ctatcatcta aaccgctcag
     6061 ggcgaagagt gccgccacga ttttgctcca gccggcaaaa taagcggata ttacttgggc
     6121 tggagccatt gtcaagttaa ggaatgatag tagcggagcc ggcgtgaaat cacacgctcg
     6181 tttcacccat cattagccgc tgcacgggtg tctacttcgc agggtaggtc tttgccagct
     6241 gatcgataat accgctttcg tctaactggc gcaggatccg atcgtcaatc atatctccta
     6301 ccttgacctt gtcgagagca gggttacgaa tcttcatgag ccgctgagtt gcgcagccct
     6361 tcaacgacag gaaaggtttt cgttccacac cattgataat atcctgtagt cgtacggcgc
     6421 ggatctttgg gcgctttgat gttcggagcc gcatagcgaa ggcgacgggt gagcccgccg
     6481 atggtcttta aatccgcccc gccgaccacc gccgccagca cggtggtcgg cagtgtatgc
     6541 ggcgtcacct ctcccgtcac gtctgagcat tgggcccttc tacttgacga actgctcgct
     6601 gggaaaattc gcggttgcga caacaccgcc gtccacgact aagggatggc catagataaa
     6661 atcagaatcg tcgctgacca gaaacagcgc agccttggct tgatcctcgg cagtgcccaa
     6721 ccggcccgcg agattttgga aagtcctgct tccggcaacg tcgtcgtcat agcctaccgg
     6781 cgaacctgaa cggttcggca ccacgcaatt gacgcgaatc ttatacggcg aaagctgcac
     6841 ggccatagcg cgagtcaggt tgatgacgcc gcccttagcc gcggcataag caatgccgtc
     6901 ggtgcgaccg agcatgccag aggtggaacc gaagttgacg atctttccac ctcgtccctg
     6961 cttggccatt tgcgcggcaa cgtgtttcgt tactaggtaa ggccccttga gactcaccgc
     7021 gatcacttta tcgaactcgt cttcggtgct ggtgaagata tcggcatgat ccgtgagcgc
     7081 ggcattgtta accaggatgt cgataccgcc gaaacgcgcg acaactttct gaagcatctg
     7141 ttgcacgtca cttgaattgg caacatcgca gagcacgagc agggcttcgt ggccgctgtt
     7201 ttcgagctcc tggaccaccg cctggccgcg gccttcgtgc gtctctatca ccgcgattct
     7261 cgctccttcg ttagcgaata gtttggcgat cgctttgccg atattccttc cggcgccggt
     7321 aaccaccgcc acttggttct gcaacttcat tgatctctcc ttctatgaaa cgagctaggg
     7381 caactaagca atttgctgct gggggagatc ctttatcaga tcttgaactc gtccggcgcg
     7441 gttgcggttg ctatatcgaa cgtattgagc gtgcacgcga gcatcgcata gtacccgact
     7501 gtagcggtga tctcgaccag gccgcgctcg cctagtcgac cgtaaagcgc ttgaaaggtg
     7561 gtttcggtaa cccggtgcgc gcgcaacaac tcgcggacga agctgacgat ctgcgcgtct
     7621 tcgaaggtaa aatgctcggc atctttagat tgatgaacgg ctcggatcgt atccagggga
     7681 atacctgcct tctgcgcatg atttacgtgt gcggcccatt catgcttgca ttgaagctcg
     7741 cgcgccgata ccagcgcggc cagttctatg accttagggt ccaaactgga ctcgaagcgc
     7801 acataactgc ccagatgcgc cgttcgaccg gcaagctcag ggctgtgcag gagcgccaga
     7861 aagggtcctc cgacgacgcc gcggctttgc gcaatcgcct ggtaaactgg ttgatgctcc
     7921 ggctgcaagt cttctttctt ttctatgata gcgattcgcg ccataggtct ctccttctca
     7981 accgaatttg tcctatatcg tgacggttcg tcggatagcc tcattgctcg agccgtcata
     8041 gcctcattgc ttaacttttc gtcgctaagt tagtggtaat cgtatccgca gaaacctgct
     8101 gccgcttgcg atcgctgacg gaagttagct aacaggtcga acttgggacc gaattagcag
     8161 aaacagcgca atcagcgcaa gccccgtagc cgaggatcaa ggacttcgcg cagtccttcg
     8221 ccgaccaaat agtaggcgag cacggtaata aagattgcca accccggaaa caccgccagc
     8281 caccaggcaa aagttgcgct gctcgcttcg tgcaggatgg aaccccaggt gaccagttcg
     8341 gcgggcacgc cgatgccaag gaagctaaga ctggactcga ccagaatggc cccggcaacg
     8401 ccgagcacaa ccgaaacgat taccggagcc agggcattgg gcagaatatg cttgaacatc
     8461 acctttcgat tggagacgcc gagcgcgagc gcagacgtaa cataatcgag actacggatc
     8521 ttgagaaatt cattacgtgt aaaccgagcg atgccgaccc agctggttgc gccgatgatc
     8581 agcatggtca agtaaatgct cggcggcaac aacgccgcca ccgtaatcaa caggaagaac
     8641 gtcggaatcg ccagcatgat ttcaaacaga cgcgagatca ccagatcgat ccatgagccg
     8701 aaatagccgg caatggctcc aagagtgata ccgatcagca ccgcaatacc aacggcgaca
     8761 aatccgatcg acagcgatac tcgagaaccg tgaatcattc cggccaagac atcgcgcccc
     8821 agccggtcgg tgcccagcca atgcttcggc gaaggatttt caaacggcgc cgtcagatcg
     8881 atatcggtgg aaagataggg caccggtgga aatattccgt gcgcgccttc gagcttcttg
     8941 aagtcgacgt tgagcagttc cttgggccat tggccaaggc cggctctgac caagtaactg
     9001 cgaaagatcg gaaagtaggt ttgctcccga tatgacaaat agtacggttt gttattggcc
     9061 agaaaatcgg cgaataaggc aactaaaaac aaaaaacaga caacgtagaa actgatgcga
     9121 gcgattggat tgcgcttaaa ctgtttccag acaccggtcc ataagccttc agcgctaact
     9181 gcgctgccac cggccaaatc ggctgtttcc ggtgatgaag catcgttggt cgtcgaaaca
     9241 ggcgtca
//
LOCUS       DASUTG010000747         5938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1744770, whole genome shotgun sequence.
ACCESSION   DASUTG010000747 DASUTG010000000
VERSION     DASUTG010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744770"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..802)
                     /locus_tag="VF089_17265"
     CDS             complement(146..802)
                     /locus_tag="VF089_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175764.1"
                     /translation="MLRNRFTVLIIVLYFSWALAVLNPVGAQSPRHIKVLVDSQQQAK
                     ANQEEVQGAGSVIIRRGGAQPSGRVTAGNRQTTVQRSTGIFTLVQDGGESILTVATRL
                     PQNQVSFYRDYAGRVGYIDRRITFTDVGTSLRVAANALAESQIQVRLTPRISYFSAEG
                     SGAIDFTEATTELIVPDGEPVSLGGSTSRIHELTRKILGYSERTSSSETELIITATMQ
                     "
     gene            complement(961..1938)
                     /locus_tag="VF089_17270"
     CDS             complement(961..1938)
                     /locus_tag="VF089_17270"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175765.1"
                     /translation="MNPQRSHKANKRLALVFSISVAFILLLGSSVRAAEGLQKIRVGF
                     PSLAFSYMPYYVAQEKDLLKKYGLEAEYIQMRTGIQPQAVINGNINFFPSVSTGISAA
                     VSGLPLVVVLNFCDGAPWILVTQKDINKPQDLIGKQVAVSGLRTSPHYFLQAALKKWE
                     IAEKDVGIISTGGTADSFIALASNRVAGTVLTPPFDDKAVSLGYKKFMFLGDLVDIPY
                     VGVVTSQAQIKNNRETVRRTLAALTDSVAWIRTNRAESSKMIADKFKVSAAEAEQTYN
                     TLIKILNKDGRLDPKVARGYLDLLRQERPIAADFDPQKVLDFSLLPGNR"
     gene            complement(1960..3159)
                     /locus_tag="VF089_17275"
     CDS             complement(1960..3159)
                     /locus_tag="VF089_17275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175766.1"
                     /translation="MDNFDDVLVVDADGHVYEGNVDLASRMPEKWRSQAPVRLKDNQG
                     NSRILLEGRIWSASQGPGPGVSGPMTEKARGYRPGMVDPLARLKDMDEEGIDVAVLFG
                     TQLALTVNGLMSGELATVLCRAVNEWLLEYCSVDPKRLLAVGLIPCQAPEAAVKELEF
                     LAKAGAVTAMLPTNVYGRNMGDSMFDPIYDCAQSLGMTLSVHPQTGHDGVPGVSGVMG
                     AGSERFFKYVYVHMTAFPFELMIAMMHMIGEGVFDRYPKLRVGFMEGGAGWLPFWAER
                     FDEHIEKLAPQMPELKRRPSEIIRGEQVVLTCESEETGLDRVFAANGENTVLYASDYC
                     HWDCHFPYSVKDVVEGQDLTFSQKERLLNRNAIEFFKLKNLPQPAALKKARQSWASMD
                     QQKIATA"
     gene            3468..4670
                     /gene="cofG"
                     /locus_tag="VF089_17280"
     CDS             3468..4670
                     /gene="cofG"
                     /locus_tag="VF089_17280"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX6175767.1"
                     /translation="MQQALKECLQGKLLEPSEALALCNVEADELHELLATAAQLRDMH
                     KGRVVTFSPKIFVPLTNLCRDFCGYCTFRKAPEESGAKTMTLEEVVRVARQGKLLGCT
                     EVLFSLGDKPEAIYPQMKAFLAERGFQRTLDYLFEACRTVLEETELLPHANPGVMGKG
                     DLQRLKEVNVSMGLMLESTSERLLLSGGAHDNAPDKNPKVRLRTLEKAGKLRIPFTTG
                     ILIGIGETWQERIAALFAIRSLHERYGHIQEVIIQNFRAKPTIPMRLHPEPAAADMVK
                     SIALARLIFGGRMNIQAPPNLTPDGYELYLDAGINDWGGVSPLTPDFINPEAPWPALQ
                     RLQEKSVSAGFELRARLPIYPEYIEQAEKFLFPSILPYVSQICGDDRLVRNNGPLGTN
                     PETQPVEA"
     gene            4691..5617
                     /gene="cofD"
                     /locus_tag="VF089_17285"
     CDS             4691..5617
                     /gene="cofD"
                     /locus_tag="VF089_17285"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX6175768.1"
                     /translation="MILALTGGTGGAKLVQGLAAEIDPADLTIICNTADDAIFHGLQV
                     SPDIDTIVYTLAGLNDEEKGWGLAGESFVALEQLRRLGQEVWFKLGDKDLATHILRTN
                     LLRQGLTLSEVTRRIRTALGVKSEILPMSDDRIATRIVTANGEMSFQEYFVRERWAPE
                     VKRVFFAGIGQSKPTPHVLQAIDNAAGIVICPSNPITSIAPILALPGVRQALREAPGK
                     IVGVSPIIRQAAISGPAHTLMRVNDLEPSVNGVAKGYADLLDVLMIDSEDQYFENDIE
                     KLGVKSVSANIRMNSLADKQRLARQVLALFSK"
     gene            5642..>5938
                     /locus_tag="VF089_17290"
     CDS             5642..>5938
                     /locus_tag="VF089_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6175769.1"
                     /translation="MKVTALIPVKGFRNAKQRLSAFLGSAEREALAEAMFRDVLRQVP
                     GARGILETVVVTGDDKVAKIAVSLGARVILESAETGETAAVDFARTSLKAAGREA"
BASE COUNT         1336 a         1635 c         1628 g         1339 t
ORIGIN      
        1 ggttgtcaag aacaccggcc tggttttctg agcgcgtaca acgaatcacc aatcttggcg
       61 tcgaagcagc gtaacgttgt aacgccttgc tctagttctg tgctttctcc gaacaaaatg
      121 ccctacaaac gtgcctgtcg aagcgttact gcatcgttgc ggttatgatc aactcggttt
      181 cgctgctgct ggtgcgctcg ctatatccta ggattttccg ggtgagttcg tgtatgcggc
      241 tcgtcgaacc gcctagcgag acaggctctc catcgggaac gataagctct gttgtcgctt
      301 cagtgaaatc gatggcgccg gagccctctg cagaaaaata acttattcgc ggcgtcagtc
      361 tgacctgtat ctggctctct gcaagagcat tggcagcgac acgcagcgaa gttccaacgt
      421 cagtgaaggt aattcgacga tcgatgtagc cgacccggcc ggcgtaatcg cgatagaagc
      481 ttacctggtt ctgcggcagt ctcgtcgcca ccgtcaaaat cgactccccg ccatcctgca
      541 ccagggtgaa aattccggtg gagcgctgaa ccgttgtctg ccggttaccc gccgtcaccc
      601 ggcccgatgg ttgggcgccg cctctccgga ttataacgct gccggctcct tgcacctctt
      661 cctggttggc tttggcttgt tgctggctat cgaccagcac cttgatgtgc cggggtgact
      721 gcgcgccgac gggattgaga acggctagcg cccaactgaa atacagtacg atgatcaaga
      781 cagtgaatct gtttcgcagc atggacgttc agtatctgca gtgtgttgaa aaacttgttt
      841 gaaggctgct caaaagacct cagatgaaag cgcgcgaagt tcgatgtgag gtgtacgcct
      901 tcgtgcgttg aagcgagacg atcgagcgca acaaggcata tgagtctttt cagcagcctg
      961 ctagcgattg cccggcagca gagaaaagtc aaggaccttt tgcgggtcga aatccgccgc
     1021 aatcggccgc tcctgtcgta acagatccaa gtagccgcgc gcgactttgg gatctaatcg
     1081 tccgtcctta ttaagaatct tgatcaatgt attataggtc tgctcggctt cggcggcact
     1141 gactttaaac ttatcggcaa tcatcttact gctttcagcg cgattggtgc gaatccaagc
     1201 gacggagtcg gtaagcgcgg ccagcgttct gcgcacggtt tcgcgattat ttttaatttg
     1261 cgcttgtgaa gtgaccacgc caacgtaggg gatgtcgacc aagtcgccca ggaacatgaa
     1321 cttcttatag cccagagaaa ccgccttgtc atcgaacggc ggcgtcagca ccgtgcccgc
     1381 gacccggttg ctggcaaggg cgataaagct atcggcagtg ccgcccgtgc tgataatacc
     1441 gacgtccttc tcggcgatct cccatttttt gagagccgcc tgcaaaaagt aatgcggcga
     1501 tgttcgcaat cccgagaccg cgacttgctt gccgattaga tcttgcggct tgttgatatc
     1561 cttttgtgtg accaggatcc acggtgcgcc atcgcaaaag ttcaatacca ccaccagcgg
     1621 caggcccgaa acggccgcgg aaatcccagt cgataccgat ggaaaaaagt tgatattacc
     1681 attgatgacg gcttgcggct gaatgccggt gcgcatctga atgtactcgg cttcgagacc
     1741 gtatttcttc agaaggtctt tttcctgggc gacgtagtac ggcatatacg agaacgccag
     1801 ggagggaaaa ccgactctaa tcttttgcag gccctcggcg gcgcgaacac tgctgcccag
     1861 gagcagaatg aaagctaccg agatgctgaa gacgagcgcg aggcgtttgt ttgctttgtg
     1921 cgagcgttgc ggattcatca aagctcctca acaaaataat caggcggtgg cgatcttttg
     1981 ctggtccata cttgcccagc tttgccgtgc cttcttaagc gcagcgggct gcggcaggtt
     2041 cttgagtttg aagaattcga tggcattgcg gttcaacaat ctctccttct gagaaaaggt
     2101 gagatcttgc ccctcgacta cgtccttcac cgaataggga aagtgacaat cccaatgaca
     2161 atagtccgaa gcgtagagaa ccgtgttttc gccgttggcc gcgaacaccc gatctagccc
     2221 agtctcttcc gattcgcagg tgagcacaac ctgctcgccg cggatgattt cgctgggacg
     2281 acgtttcaac tccggcattt gtggcgcgag tttctcgata tgctcatcaa agcgttcagc
     2341 ccagaatggc aaccagccgg cgccgccttc catgaacccc acgcgcagct tcggataacg
     2401 gtcgaacact ccctcgccga tcatgtgcat catcgcgatc atcagctcga aaggaaatgc
     2461 ggtcatgtgc acgtagacgt acttgaaaaa ccgttcgctg ccagcgccca tgacgccgga
     2521 gacacccggc accccgtcgt ggccggtctg gggatgaacg ctcaaagtca tgccgaggga
     2581 ttgagcgcag tcatagatcg ggtcgaacat cgaatctccc atgttgcggc cgtagacatt
     2641 ggttggcagc atcgccgtga ccgcgccggc tttggcgaga aactccaatt ctttgactgc
     2701 cgcttccggc gcctgacatg gaatcaggcc gacggcaagc aagcgtttgg ggtccacgga
     2761 acaatactcc agcagccatt cattgaccgc gcggcaaagc acggtagcca gctcaccgct
     2821 catgagccca ttcaccgtca gcgcgagttg cgtgccgaac agcacggcga catcgatgcc
     2881 ttcctcatcc atatccttca atcgtgctag cggatcgacc atgcctggcc gatagccgcg
     2941 cgctttttcg gtcatcgggc cgctgacacc gggaccggga ccttgagaag cggaccagat
     3001 gcgtccttcc aacaggatac gtgagttacc ctgattgtct ttaagtcgca cgggagcttg
     3061 cgagcgccac ttctcgggca ttcggctggc gagatcgacg ttgccttcgt agacatggcc
     3121 gtcagcatca accacaagca cgtcgtcaaa gttatccatg atccgttccc ccctgacact
     3181 ttttctgatg ttctatcttc acgctaacga ttcggcaaca gggccgccaa aatcgtggtg
     3241 ccgctttgct ggtgaaattc gcatcttatc aagcgagact cgtatcatcc agaatagctt
     3301 cgctcaagcg agtcattaga cctgctttgt tggtgttgac gctgtcctta gtcgctacgc
     3361 cagctcgggg tcctgagacc cttaccctct tgatcattgc gaacagcatg attttacgat
     3421 tgaagttgca gttgaccgca ttatgccgat ccgatatgca caggctgatg cagcaagcac
     3481 tgaaagaatg tcttcaagga aaattgctcg agccatccga ggcgcttgcc ctttgcaacg
     3541 tcgaagcgga tgaattgcac gaattgcttg ctaccgcggc ccaacttcgc gacatgcaca
     3601 aaggcagagt cgttactttt tcgccgaaga tatttgtgcc attgacgaat ttgtgccgtg
     3661 acttctgcgg ttactgcacg tttcgcaaag cgccggagga atccggcgcc aaaacgatga
     3721 ccctggaaga agtggtccgt gtggcgcgcc agggaaaatt gcttggctgc accgaagtgc
     3781 ttttcagttt gggtgacaaa cctgaagcga tctatccaca gatgaaggcc ttcttagcgg
     3841 aacgcgggtt ccaacgcacc ttggattatc tatttgaagc ctgccgaacc gtgcttgaag
     3901 aaaccgagtt gttgccacac gccaatcctg gggtaatggg caagggcgat ttgcagcgat
     3961 tgaaagaggt taatgtcagc atggggctga tgctcgagag caccagcgag cgtttgttac
     4021 taagcggtgg cgcccatgat aatgcgccgg acaaaaaccc caaggttcgt ttacgcactc
     4081 tcgagaaagc cggcaagctt cgcattcctt ttaccaccgg tatcctgatc ggcattggcg
     4141 agacttggca ggaacgcatc gccgcactct ttgcgatccg cagtctgcat gaacgttacg
     4201 gccatattca agaggtgatt atccagaact ttcgcgccaa accgaccatc ccaatgcggc
     4261 ttcatcccga gcccgcagca gcggacatgg tgaaaagcat cgctttggca cgtttgatat
     4321 tcggcggccg catgaatatc caagctccgc ccaatctgac accggacggc tatgagcttt
     4381 atcttgacgc cggtatcaat gattggggcg gcgtatcgcc gctgaccccc gacttcatca
     4441 atcctgaagc gccatggccg gcgctacagc gattgcagga aaagagcgta tcggcgggat
     4501 tcgagctgcg agcgcgatta cctatctatc ctgaatacat tgagcaagcc gagaaattcc
     4561 ttttcccaag tattctgccc tatgtcagcc aaatatgtgg tgatgaccgc ctggtaagaa
     4621 acaacggtcc actgggcaca aatccggaga cccaaccggt cgaagcctag ccgtcatagc
     4681 ccgtttagcc atgatcttag ctttaaccgg cggtaccggc ggcgccaaac tcgttcaagg
     4741 tctcgcggcg gaaatcgacc ccgccgacct gacgatcatc tgcaataccg cggatgatgc
     4801 gatctttcat ggactccagg tttccccgga catcgatacg attgtctata cgcttgccgg
     4861 tctcaatgac gaagaaaagg ggtggggcct ggctggcgaa agcttcgtcg ctttggaaca
     4921 gctccggcgc ttgggccagg aagtatggtt caagctcggc gataaagatt tagcgacgca
     4981 tatcctgcgc acaaaccttt tgcgacaagg gctcacctta tcggaagtga cacggcggat
     5041 tcgcactgca cttggggtca aatcggagat tctgccgatg tcggacgatc gcatcgcaac
     5101 ccgaatcgtg acagcgaatg gggaaatgtc attccaggaa tattttgtgc gcgagcgctg
     5161 ggccccggag gtcaagcggg tgttcttcgc tggcatcggt caaagcaaac cgacaccgca
     5221 cgtgcttcaa gcgattgata atgctgccgg gatcgtcatt tgtccgagca atccaatcac
     5281 aagcatcgcg ccgattcttg cgctgcccgg cgtgcgtcag gccttaagag aagctcccgg
     5341 aaaaatcgtc ggtgtcagtc cgattatccg gcaggcggcg atcagcggtc cggcgcatac
     5401 tctgatgaga gttaacgatc tggagccttc ggtaaacggt gtggccaaag gctacgccga
     5461 tcttctagac gtgctgatga tcgacagtga agaccagtac tttgagaacg atatcgagaa
     5521 gctgggggtc aaatcggttt cagcaaatat ccggatgaac agcctcgccg acaaacagcg
     5581 gttggcacgc caagtcttgg ccttgttttc gaaatagtgc taagaaatct cccggtaaac
     5641 aatgaaagta accgcactta ttcccgtcaa agggtttcgg aacgccaagc agcgtttgag
     5701 cgcgtttttg ggcagcgctg agcgggaagc ccttgcggaa gcgatgtttc gcgatgtgct
     5761 gcgtcaggta ccaggcgcgc gtgggatact cgaaactgtc gtggtgacgg gcgatgacaa
     5821 agtcgcaaag attgccgtat ctttgggcgc aagagttatc cttgagagcg ccgaaaccgg
     5881 cgaaaccgcc gccgtcgatt ttgcgcgtac cagcctgaaa gctgccggcc gcgaagcg
//
LOCUS       DASUTG010000748         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1595419, whole genome shotgun sequence.
ACCESSION   DASUTG010000748 DASUTG010000000
VERSION     DASUTG010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595419"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF089_17295"
     CDS             complement(<1..855)
                     /locus_tag="VF089_17295"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6175770.1"
                     /translation="MKRLRVLSAALSLVTAIVWSAPFAGAQDRQSKGRSKDAQQQNRM
                     WHFIVSAWDLIGSPVYDSKGERAGEIEYLMIGMDNGAVRYVVVSSGGFLDIGDELRAV
                     PWSAFDTPVSRTGARLDVTRKALQESYKTDQESLSDLTKPTVIAQVTEYWAPLMEEQS
                     GEKKTGSKSQSESKSKAATSKGQKQEQKAGDRAEAKAKSSSSSAAKSDSKNQTAKKDE
                     AGVPHILVGQGLITTVVAPAVQSAKELVGAEVRSADGKEVGEIDHLMIETSVGRVAYV
                     LISRGGFLG"
     gene            complement(1247..3256)
                     /locus_tag="VF089_17300"
     CDS             complement(1247..3256)
                     /locus_tag="VF089_17300"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6175771.1"
                     /translation="MLQASVDGLFLVLSWKSLSLMLVGIAVGFSVGILPGLGGPVTLA
                     LMLPFVFKMEGVEAFAFLLGMSAVTATTGDITSILFGVPGEATTASTIVDGHPMAKKG
                     EAGRALGAALMSSLVGALFGALCLGLAIPVVRPLVLSVGSPEFFMLTLLGVTFLATLS
                     GDAVIKGLTAAAIGLQFATVGLDPISGIERYTFGTLYLWDGVRLVPATLGLFAIPELI
                     DLAVKGTSIAETKFDKLGGVLQGIKDTFHHWKLVLRCSAIGAYIGVIPGMGGAVSQWV
                     AYAHAVQSSPDRERFGKGAVEGVLGPGAANNSTLGGALVPTLAFGVPGSVSTAILLGA
                     FIIQGLVPGPPMLLPESQGGSLSLTFSFVWILVLSNIVTVSICFLFLNQLARITQVRG
                     SLVIPFILLLIYLGGFAENNTLQDLIVVSLFGALGWIMVKLDWARPPLLLGLVLGPLA
                     ENNLFLATDNYGVAWLGFPSVLMLGAIILAGMFFPIFQTWHRKRRKNPPMVERAPVLS
                     VEKEPVISLWAPIFTLSVVLFFAWTLWEGREWWFRARLFPWTIGFAGLGLALVQLYLD
                     VKYLVKTKGARRAEAPAPESALAMRPTLAITGWILGFFAAIWLLGFPIAVPLSMFIYL
                     KAGARERWPMAVALTFFAWIAFYGLFDYALHVPFPPGLLFEWLRV"
BASE COUNT          815 a         1003 c          882 g          669 t
ORIGIN      
        1 acccaggaat ccgccacggg agatcagcac ataagcaacg cgtcccacac ttgtctcaat
       61 catcaagtga tctatttcac ctacctcttt gccatccgcg ctgcgcactt ctgcgccgac
      121 cagttccttc gcagattgca cggccggtgc cacgacagtg gtgataaggc cctgcccgac
      181 cagaatgtga ggcacgccgg cttcatcctt ctttgccgtt tggtttttag agtctgattt
      241 ggccgcagaa ctgcttgagg acttagcctt ggcttcggct cggtcgccag ctttctgctc
      301 ctgcttttgc cctttggaag ttgctgcttt ggacttcgat tcggattgag atttggatcc
      361 ggtctttttc tcaccgctct gctcctccat cagaggagcc caatactcgg tcacttgagc
      421 gatcacggta ggcttggtta aatcgctcag gctttcctgg tcggtcttat aactctcctg
      481 aagcgctttc ctcgtaacgt cgagccgtgc gcctgtgcgc gacaccggag tatcgaacgc
      541 cgaccaaggc accgctcgca gctcatcgcc gatatccaaa aatccgccac tcgataccac
      601 gacgtaacgc actgcaccat tatccatgcc gatcatcagg tactcgatct cgccagctct
      661 ttcccctttg gagtcgtaca ccggactgcc gatcaagtcc caggcagaga cgatgaaatg
      721 ccacattctg ttctgttgtt gagcgtcttt tgaccgtcct ttactctgtc gatcctgagc
      781 accggcaaac ggcgccgacc aaacgatggc tgtcacaagc gacaaagccg cgcttagcac
      841 gcgcaaacgt ttcataagaa aaccctcctc tttgtttgat ttgctcatga tgttcgcaac
      901 cccagtgcca ctgtttatgt tccgttcgca gctggtgcat tgtcctcctc ggcagcttcg
      961 gacgacgcta ttcacttgat cgtgcgcagc gaaaacacac acgagtcacc tattgtgcac
     1021 agcgggatca cggacgaatc tctcaacgtc tcgcttggct cattaggcgg agatacatgg
     1081 ctgtggcctc agccgatgcg cttggcgact gcaactctca acaaggcacc aacaagaaca
     1141 gaacgtgatg cacccttgga gaccgctttg attctgcagc gcactgcggc atcaacaccg
     1201 aatcattgaa cttgtatcga tggatatccc cggtaggtcg caaagcttaa acccgcagcc
     1261 attcgaacag taggccaggg ggaaatggaa catgcagggc gtaatcaaag agcccataga
     1321 atgcgatcca ggcaaaaaac gttaaggcga cagccatcgg ccatcgttcg cgcgcgccgg
     1381 ccttgagata gataaacatc gatagcggaa ccgctattgg aaaaccgagc agccatatgg
     1441 cggcaaagaa ccccaggatc catccggtga tcgccagggt cggccgcatt gccaaagcag
     1501 attcaggtgc cggcgcttcg gctcgtcgcg cgcctttggt tttcactaaa tatttcacat
     1561 ctagatagag ctgtacgagc gccagcccca gcccggcaaa cccgatcgtc caggggaaaa
     1621 gacgggcgcg gaaccaccac tcgcgccctt cccacaaggt ccaggcgaag aataagacca
     1681 ccgataaagt gaagatcggc gcccacaagg aaatgaccgg ttctttctct actgaaagca
     1741 caggtgctct ttctaccatc ggcgggtttt tccgccgctt tctgtgccat gtctgaaata
     1801 ttggaaagaa catgccggca agaatgatcg cccctagcat gagcacgcta gggaaaccaa
     1861 gccaggccac gccatagtta tcggtggcga gaaacagatt gttttccgcc aacggcccaa
     1921 gcacgaggcc taacagcaga ggcgggcggg cccaatcgag tttaaccatg atccagccga
     1981 gcgcgccgaa cagcgaaacc acaatcaaat cttgcagggt attattttca gcgaagccgc
     2041 cgagatagat caacaacagg atgaaaggaa tcaccaggct tcccctcacc tgggtgatcc
     2101 tggcgagctg attgagaaag agaaaacaga tgctaaccgt gacgatattt gacaggacga
     2161 gaatccagac aaacgaaaaa gttagcgaaa ggctgccgcc ctggctctcc ggcagcagca
     2221 tcggtggacc tggaacgagt ccttggatga tgaaagcgcc gagaagaatc gcggtgctca
     2281 cgctgccggg cacgccaaag gcgagagtag gcaccaaggc cccaccgaga gtggaattgt
     2341 tggccgcgcc gggacccaaa actccctcca ccgctccctt accgaaacgt tccctatctg
     2401 gcgagctttg caccgcgtgc gcgtaagcca cccactgaga aacggcgccg cccatacccg
     2461 gaatgacgcc gatgtacgcc ccgatggcgc tgcagcgtag gaccagtttc cagtggtgga
     2521 aggtgtcctt gattccctgc agtacgccgc ccaatttgtc gaacttggtt tccgctatac
     2581 ttgttccctt gaccgccaag tcgatcagtt caggtatcgc gaaaagtccg agggtcgctg
     2641 gcaccagccg aaccccgtcc cacaagtaaa gagttccgaa agtgtatcgt tcgattcccg
     2701 agatcgggtc caatcccaca gtggcaaatt ggagccctat ggctgcggcg gtaaggcctt
     2761 tgatcaccgc atcgccgctt agtgtggcaa gaaaggtgac gcccaacagc gtgagcatga
     2821 aaaattcggg agatcccacc gaaagcacca gaggacgaac aacaggtatc gctaacccca
     2881 ggcacaacgc gccaaagagc gcgccgacca gcgagctcat taatgccgct ccgagagcgc
     2941 gtcctgcttc gccctttttt gccatcgggt gaccatcgac gatggtcgag gcggtagtgg
     3001 cctcgcccgg aactccgaac agaatagagg tgatgtcccc ggtggtggcc gtgaccgcgg
     3061 acattcccaa aaggaaggca aaagcctcga caccctccat cttgaaaaca aagggtagca
     3121 tgagcgccag ggtcaccggt ccgccgaggc ccggcagtat acccacggaa aaaccgacgg
     3181 cgatgccgac aagcatcagg ctcaggctct tccacgaaag caccaggaac aagccgtcga
     3241 cagaagcctg caacatctcc agcagcataa tggtttagag gggctcagtt attggacaat
     3301 cgcatgcaga gaatcggggc atgctgctgc ccatagagct agactgcggg gccggctcgt
     3361 ataatcaca
//
LOCUS       DASUTG010000749         9895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_707771, whole genome shotgun sequence.
ACCESSION   DASUTG010000749 DASUTG010000000
VERSION     DASUTG010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707771"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..691)
                     /gene="ilvN"
                     /locus_tag="VF089_17305"
     CDS             complement(170..691)
                     /gene="ilvN"
                     /locus_tag="VF089_17305"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6175772.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGYNIESLSVAATLDPTTS
                     RITIVTIGDDRIIEQIMKQLNKVVEVLEVVDLTETDFLDRETALIKVHTKPEHRDEAV
                     RIADIFRAKIVDSSLTTYTIEITGDVDKVEALINMLKPLGIKELIRTGRIGIARETTR
                     TASPRREPALLKS"
     gene            complement(702..2480)
                     /gene="ilvB"
                     /locus_tag="VF089_17310"
     CDS             complement(702..2480)
                     /gene="ilvB"
                     /locus_tag="VF089_17310"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX6175773.1"
                     /translation="MKITGAEIFCESLRREGVKTVFGLPGGVVLKIFDVLCQQKDIEL
                     VLTRHEQGAAHMAEGYAKATGKAGVVLVTSGPGMTNIVTGLADAYMDSVPLVAFTGQV
                     STDLIGNDAFQEADNVGISRPCTKHNVLVKDVNDLAQTIKEAFYIATSGRPGPVLVDI
                     PKDVSTAKAEFKYPEKISLRGYNPVIGGSKQHIRQAAEEIMKAKKPMIYVGGGAIFSN
                     AADEIRELAEITQIPVTMTLMGLGSFPGTHPLSLGMLGMHGGYWTNMAMHHADLLIAV
                     GARFDDRVTGKLSEFCPEARVVHIDIDPTSIKKTFHAHIPIVGDVKAVLRQMNVMLRS
                     MDGNPAKIRASRAPWLKQVEEWRTAHPLSYKQDDKIIKPQYVIDKLFELTKHEAIVAT
                     DVGQHQMFAAQYFKGTKPRTWLTSGGLGTMGFGFPAAIGAQKAFPDRMVLCITSEGSF
                     QMNLQELVTCAEQKLAVKIVLLNNGVHGMVRQWQDLFYEGRYSASLLGKMPDFVKLAD
                     AYGIKGLRAVKPSEVEPVLKEGLKHKGPVLLDIHTDPYENCYPMIPAGGAQHEMMLED
                     PPELIRGKKGGAQKRKVEEGEGVLPA"
     gene            complement(2497..2727)
                     /locus_tag="VF089_17315"
     CDS             complement(2497..2727)
                     /locus_tag="VF089_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX6175774.1"
                     /translation="MENSEKMAIASLLDRDPELKQYYEEHQELEKKLDQFQNKLHLSP
                     DEEIEKKRLQKMKLAGKDKIMEILGRHRQTGA"
     gene            complement(2819..3556)
                     /gene="tsaB"
                     /locus_tag="VF089_17320"
     CDS             complement(2819..3556)
                     /gene="tsaB"
                     /locus_tag="VF089_17320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6175775.1"
                     /translation="MRILGLDTSIAVASVAISEDGRIVAEDFYPRHATAERSVAKSNH
                     AEIILPLIDSVLQRAGTGLSDVTGIAVSIGPGSFTGVRIGLSTVKGLAYGTGMPAAGI
                     STLQAIAARVAGFEGIVCPILDARKGEVYASVFRKHGNRLERLTEDALMPILSFLREL
                     RGIEKTGRCLFIGAGVAAYGPLLQRAADLQISFAEEEGMPSVAASIALLSERQFIDDR
                     PVQLADLAPVYLRRPECESRMKATVSS"
     gene            complement(3553..4635)
                     /gene="rseP"
                     /locus_tag="VF089_17325"
     CDS             complement(3553..4635)
                     /gene="rseP"
                     /locus_tag="VF089_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX6175776.1"
                     /translation="MESLLYSILAAVVGLGVLIVFHEFGHFLLAKLSGVGVLTFSVGF
                     GPKLWVKKKGETEYALSAFPLGGYVKMVGEDPEEEVKAVDLERSFAHKSLLKRTAIVA
                     AGPGFNLLLAVFLLMVVFLFYGVPVLSNLVGAVEPESPAAQAGIQKGDRIVAVDGQAV
                     TGWDDLSTAIKQSSGKPLGLRLQRDGQEMTMTVQPRKREVKNIFGELKEDWMIGIGSQ
                     VSIEKGDPGLAISRAFVQTYEYSKLTLIGLYKMITREVSPRNLGGPILIAQMAGQQAQ
                     EGIGSFLAFLAVLSINLGVLNLLPVPVLDGGHLFFFAIEAIIGRPVSLKYREKAQQVG
                     IFLLLLLMIFAFANDIFRLFEKPVGG"
     gene            complement(4653..5801)
                     /locus_tag="VF089_17330"
     CDS             complement(4653..5801)
                     /locus_tag="VF089_17330"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6175777.1"
                     /translation="MRSIALLGSTGSIGVSTLDLVRRFPDRFRIFGMVAGQNLKLLAA
                     QIKEFAPQCVALKNESDVPRLRKLLGKKNIEIGWGVKGATAISTANEVDVVLAAIVGA
                     AGLVPTLEAIRAGKEVALANKEALVMAGEILVNEAKRAKSRLFPVDSEHSAIFQCLEG
                     NQRDEVDKLILTASGGPFLRTPLNQLSRVTISQALKHPNWKMGRKITIDSATMMNKGL
                     EVVEAHWEFNMEPSRIDVVIHPQSVVHSMVRYQDGTIIAQLGIPDMRIPIAYALAYPH
                     RLNGGWKPLEMTDHGELNFLPVDKKRYPALGLAYDALKQGGTMPAVLNAANEIAVEAF
                     LTGKIGFREIHRTIERTMCNYSPRAARDVEDVLDADRWARERAASLVH"
     gene            complement(5798..6604)
                     /locus_tag="VF089_17335"
     CDS             complement(5798..6604)
                     /locus_tag="VF089_17335"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6175778.1"
                     /translation="MDANLRARLLTGLVGIPLLVVLIGWGDPWLFHGFFLLITFGALR
                     EYFAMGFPGRPREQTIGVAFGLAVSSAVFFPQLTPGEIILAPLLVLMFSIYIFMQGQL
                     EERLARLAWILLGSLYLGYLLPHWSSLFRLPHGRAWVFFVLVVIMAGDICAYFVGRRF
                     GTKKLAPEISPGKTVEGALGYVLGSVLAGYLVGISLAMGLGWVELVILSALLSVLGQI
                     GDLFESWIKRAFAVKDSGRLLPGHGGLLDRLDSLIFPAVFTTTYLKVFHP"
     gene            complement(6588..7367)
                     /gene="uppS"
                     /locus_tag="VF089_17340"
     CDS             complement(6588..7367)
                     /gene="uppS"
                     /locus_tag="VF089_17340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX6175779.1"
                     /translation="MDSKDLDRSRLPRHVAIIMDGNGRWATLQGKSRIEGHRRGKTSV
                     RVIVEMTRKLGIPFLTLFAFSTENWLRPHDEVHALMGLLETYLIAEQPKMMRYGIRLK
                     AIGNRDRLPLNVRRTLEEVIELTERNSRMTVVLALSYSGRDEIVRMVNKIRRKILAGE
                     FQSSDVEEEIVSAHMDTPDIPDPDLLIRTSGEMRISNFYLWQMAYTELYVTPTLWPDF
                     REQEYLQALFEYQRRRRRFGRTDEQISEPARLRVGSSGRES"
     gene            complement(7467..8024)
                     /gene="frr"
                     /locus_tag="VF089_17345"
     CDS             complement(7467..8024)
                     /gene="frr"
                     /locus_tag="VF089_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6175780.1"
                     /translation="MNEAIFTQLQKEMEGSVAALRKEMGRVRTGRASTALLEGVLVDY
                     YGATTPLNQLATLSAPEPRLLVIQPFDRNALAEIEKAILKSDLGLTPANDGKIIRVPI
                     PQLTEERRKDLVKHVKKTGEEFRVSMRNHRRLAIEHLKESEKKKEITADEFKHGQERV
                     QKITDDYIARVDKVLKTKEEEIMEV"
     gene            complement(8021..8752)
                     /gene="pyrH"
                     /locus_tag="VF089_17350"
     CDS             complement(8021..8752)
                     /gene="pyrH"
                     /locus_tag="VF089_17350"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6175781.1"
                     /translation="MSEANLKYKRIILKVTGEIFAGAQKFGIDAQTVKAFAQEIKEVK
                     EIGCEMALVVGGGNIFRGAVASEIGMDRASADTMGMLATVINSLALQDALEKLGVSTR
                     VLSAIEMRQVAEPYIRRRATRHLEKGRVVIFAGGTGNPYFTTDTTASLRAMEIGAEVI
                     LKATKVEGVYDADPFKFEGATKFEQLTYIEVLNRELKVMDSTAISLCMDHQLPIVVFN
                     LMEKGNIKRVVAGEAIGTLVSAGKR"
     gene            complement(8772..9380)
                     /gene="tsf"
                     /locus_tag="VF089_17355"
     CDS             complement(8772..9380)
                     /gene="tsf"
                     /locus_tag="VF089_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6175782.1"
                     /translation="MEVSAAAVKNLREKTGAGMMDCKKALAESGGDLDKAVDYLRQKG
                     LAAASKKASRAASDGAIGAYVHPGGKIGVLVELNCETDFVARTPEFQNLLKDIAMQIA
                     AANPRYIRPEEVPTGDLEKEREIYRRQALESGKPEKVVDKIVDGKIDRFYSEVCLLEQ
                     SFIKNPDRKVSEILTDAVARLGENIQVKRFSRYQLGEGAPQE"
     gene            complement(9384..>9895)
                     /gene="rpsB"
                     /locus_tag="VF089_17360"
     CDS             complement(9384..>9895)
                     /gene="rpsB"
                     /locus_tag="VF089_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6175783.1"
                     /translation="RWLGGTLTNFSTIRQSIDRLRKLEEMENDPKIVDALTKKEMLGL
                     KREKDKLEQSLGGIKAMRKLPDAIFVVDPKQEEIAVKEARKLGIPVIAVIDTNCDPDM
                     VDHKIPGNDDAIRAIRLFCSSMADAVIEGKGLYDQSLAKDKGDEGSSAVPAPTEMEHP
                     VEQSAMGGV"
BASE COUNT         2336 a         2799 c         2286 g         2474 t
ORIGIN      
        1 attgagttcc cctttacagc gaaattctaa tatggccaga acagacgatc atgtcaattt
       61 agagctgacg tccctacagg atgttgccgc aaacggaaag aatgatgaga agcgctttac
      121 ctgacctcgc gggaggttct agcctcgaaa atagaatctg acggcggttt tacgatttga
      181 gcaacgccgg ctcgcgacga ggtgaagcgg tgcgcgtggt ctctcgcgca atgccgatcc
      241 tccccgtgcg aataagctct ttgatcccta agggctttag catattgatc agcgcttcga
      301 ccttatcgac atcaccagtg atttcgattg tatatgtggt aagtgatgag tcgacaattt
      361 tggcccggaa gatgtctgca atgcgtacgg cttcatctcg gtgttcgggt ttggtgtgaa
      421 ctttgatcag agcggtctcg cgatcaagga aatctgtctc ggtgagatcc accacttcga
      481 ggacttccac gaccttgttt aactgcttca tgatctgctc gatgatccga tcgtcaccaa
      541 tagtaacgat ggttatgcga gaggtcgtag gatccaaagt ggcagccact gataggcttt
      601 caatgttgta tccgcgcgcg ctgaacaatc ctgctactct tgccaaaaca ccaaatttgt
      661 tttcgacgag aaccgatatg atatgttcca ttcgctgaaa atcaggcagg tagaactccc
      721 tcgccctcct ccacctttct cttttgtgct ccacccttct tgcctcgaat cagctccgga
      781 gggtcctcga gcatcatttc gtgctgggcg ccgcccgcag gaatcatcgg gtaacagttt
      841 tcatacggat cggtatgaat gtccagcagc acaggccctt tgtgcttcag gccttctttt
      901 agaacgggtt cgacctcgct cggttttacc gcgcgcagcc ccttgatccc gtaagcgtct
      961 gccaatttca caaagtcagg catcttcccc aaaaggctcg ccgagtaccg tccttcgtag
     1021 aacagatcct gccattgccg gaccatgccg tgcaccccat tgttaagcag aacaattttg
     1081 acagccaact tttgctcggc gcatgtgacc agctcctgta gattcatttg gaagctaccc
     1141 tcgctggtga tgcaaagcac catccgatcc gggaaagctt tttgtgcacc gatggctgcc
     1201 ggaaagccga agcccatggt ccccagcccg cctgaagtga gccaagttct cggcttggtc
     1261 cccttaaaat attgcgcggc aaacatctgg tgctggccga catcagtggc cacaatcgct
     1321 tcatgtttgg tcaattcgaa gagcttgtcg atgacatact gcggtttgat gatcttgtca
     1381 tcttgcttgt atgacaaagg atgcgccgtt cgccattctt cgacctgctt caaccagggt
     1441 gcccgagacg ctctgatctt ggcgggatta ccgtccatcg atcgcagcat gacgttcatc
     1501 tgccgcagaa ccgctttcac atcgccgaca atcgggatat gggcgtggaa agtctttttt
     1561 atcgaagttg ggtctatgtc gatgtgaacg acccgtgcct cgggacaaaa ttctgaaagc
     1621 ttaccggtca cacgatcgtc aaaacgcgcg ccgacagcga ttagtaaatc agcatgatgc
     1681 atcgccatgt tggtccagta accaccgtgc attcccaaca tgccgaggga cagcggatgg
     1741 gtgcccggaa aactacccaa acccattaag gtcatggtca caggaatttg tgtgatttcg
     1801 gccaactcac ggatttcatc cgcagcattg gaaaaaatag caccgccgcc aacgtagatc
     1861 atcggctttt tggccttcat gatctcttca gcagcttgac gaatgtgttg tttgctgccg
     1921 ccgataaccg ggttgtagcc acgcaagctg atcttttccg gatatttgaa ttctgcttta
     1981 gcggtactca catcctttgg aatgtcgact aaaacaggtc cagggcggcc gctcgtcgca
     2041 atataaaatg cctccttgat ggtttgcgcc agatcattga catccttgac cagcacattg
     2101 tgcttggtgc agggccggct gatgcccaca ttgtcggctt cttggaatgc atcattgccg
     2161 atcagatctg tcgaaacctg gcccgtgaag gcaaccagag gaaccgaatc catgtatgca
     2221 tccgcaagcc cggtaacgat gtttgtcatg ccgggccctg aggtcaccaa aaccacccca
     2281 gcctttccag ttgctttggc ataaccctcg gccatatggg cggcgccctg ttcatgccgc
     2341 gtcagtacta gctcaatgtc tttttgctgg caaagtacgt caaagatctt caacaccact
     2401 ccaccgggca atccaaaaac agttttgaca ccttcgcgtc ttagtgattc acagaatatc
     2461 tcggccccgg taatcttcat cttgcgtcct tttcaactaa gcgccggttt ggcggtgccg
     2521 tcctaggatc tccatgatct tgtccttgcc cgccagtttc attttttgca gtcgtttctt
     2581 ctcaatttcc tcgtcaggag aaaggtgaag tttattttgg aattgatcga gctttttctc
     2641 caactcttga tgctcttcat aatattgctt gagctcggga tcacgatcca aaagagaagc
     2701 aatcgccatc ttttcactat tctccatata cgagcctcct tttatcttat gaagtttttt
     2761 gcgcgcctca atctaaccta cggtcttatt cttgtcaacg taacttaacg cgtagatttc
     2821 aactacttac ggtcgccttc atgcgggatt cgcactccgg tcgacgcaag taaaccggcg
     2881 ccaaatctgc caactggact ggccggtcat cgatgaattg gcgctcactc aggagcgcaa
     2941 ttgaagctgc cacagatggc atcccctctt cctcagcgaa ggaaatctgt aaatccgctg
     3001 ctcgctgaag aagaggacca taggcagcca ctccggcgcc aatgaataaa catcgtcccg
     3061 ttttctcgat gccgcgaagt tccctaagaa aactcaggat cggcatcagt gcatcttccg
     3121 tcaagcgctc aagccgattt ccatgtttac gaaataccga agcgtaaacc tcacccttcc
     3181 gggcatccaa aatagggcac actattccct cgaaaccggc tacccgcgca gcgatcgctt
     3241 ggagtgtcga aatcccagcc gccggcatac cggttccgta agcaagtccc ttaaccgtgc
     3301 ttagaccgat tcgaactccg gtgaaagacc ccggcccgat cgaaacggca atgccagtca
     3361 cgtcactcaa tccagttccc gctcgctgca gcaccgagtc aatgagcggc aggataatct
     3421 ctgcatgatt agacttggcg acggatctct cggcggttgc gtggcgcgga taaaagtcct
     3481 ccgcgactat tcggccatct tcactgatcg caacgctcgc aaccgcaatc gaggtatcga
     3541 gaccaagaat tctcatccgc caaccggctt ttcgaacaat cgaaaaatat cattggcgaa
     3601 cgcgaagatc atgagcaata aaagcaaaaa tatgccgacc tgctgcgcct tttcgcgata
     3661 ctttaacgaa acgggccggc cgatgatcgc ctcaatggca aaaaagaaaa gatgaccccc
     3721 gtcaaggacc ggaaccggta acagattaag cacaccaaga ttgatgctca agaccgcgag
     3781 aaacgctaaa aaacttccga tcccctcttg agcttgctgc ccagccattt gggcaatcaa
     3841 gatgggtccg cctaagttac gcggcgacac ttctcgcgta atcatcttat agaggccgat
     3901 taaagtaagc ttggagtatt cgtaggtttg tacaaaggct ctgctgatgg ccaaacccgg
     3961 atcgcctttt tcaatcgaaa cctgactgcc gataccgatc atccaatctt cttttaattc
     4021 accgaaaata ttctttactt ctcttttcct cggctgaacc gtcatggtca tttcttggcc
     4081 gtctctctga aggcgcaaac ccaggggttt tccgctgctt tgcttaatcg cggtggaaag
     4141 atcatcccaa cctgtgactg cctgaccatc aacggcgaca atgcgatcac ctttttggat
     4201 tcccgcctgc gctgccggcg attccggttc aaccgctcca actaaatttg atagaaccgg
     4261 cactccataa aaaaggaaaa ccaccatcaa cagaaacacc gcaagcagta ggttaaatcc
     4321 cgggcccgcg gcgacgattg ccgtgcgttt gagaagtgat ttgtgggcaa aggagcgttc
     4381 gagatcgacc gccttaactt cttcttcggg gtcttctccg accattttta catagccgcc
     4441 taagggaaac gcactcagag catattcagt ttcacctttc tttttcaccc aaagcttcgg
     4501 tccgaatccg accgaaaacg ttagaacgcc tacccccgaa agcttggcaa gaagaaagtg
     4561 gccgaattcg tgaaaaacga taagcacgcc tagcccaacc acagccgcta gtatcgaata
     4621 gagcagactt tccatattat ctccttaggt catcagtgaa ccagcgacgc tgcgcgttct
     4681 ctcgcccagc gatcggcgtc caggacatct tccacgtctc gcgcagctcg tggtgaatag
     4741 ttgcacatgg ttctctcgat ggttcgatga atctctcgaa agccaatctt gccggttaag
     4801 aacgcctcca ccgcaatttc attggcggcg tttaataccg ctggcatggt gccgccttgt
     4861 ttcaatgcgt cataggctaa gccaagagcg gggtaacgct ttttatccac tggcaaaaag
     4921 tttaactcgc catgatctgt catttctaga ggcttccaac caccgttgag tctgtgcgga
     4981 tacgcgagag cgtaagcaat cggaatacgc atgtccggta ttcccaattg cgctatgatc
     5041 gtaccgtctt gataccggac catggagtgg acgacactct gcggatggat caccacgtcg
     5101 atgcgtgacg gctccatatt aaattcccag tgtgcttcaa cgacctcaag ccccttgttc
     5161 atcatcgtcg ccgaatcgat ggtgattttt cgacccatct tccaattagg atgcttcaag
     5221 gcctgagaaa tcgtcacacg actcaactgg ttgagcggtg ttcttaaaaa cggtccccct
     5281 gacgcagtga gaatcaattt gtcaacttcg tcgcgctgat ttccttcaag acactgaaat
     5341 atggcgctat gctcactgtc gacaggaaag agccgcgatt ttgctctttt agcttcatta
     5401 acaagtatct ctccggccat caccaaagcc tctttgttgg caagcgcaac ctccttgccg
     5461 gcgcggatgg cttcaagcgt gggtacgagt ccagcggccc cgacaatcgc cgcgagcacg
     5521 acatccacct cgttggccgt cgaaatagct gtagcgcctt tgacacccca gccgatctcg
     5581 atatttttct tacccagcaa cttgcgaagc cgcggcacat cgctctcatt tttgagggcg
     5641 acacattgtg gcgcgaattc tttaatctga gcggcaagaa gcttcaagtt ttggccggcc
     5701 accatgccga aaattctgaa tcggtcagga aatcggcgca ccaaatccaa agtgcttact
     5761 ccgattgagc ctgtcgaccc gagtaaagca attgatctca tggatgaaag accttcaagt
     5821 aagtcgttgt aaaaacagcc gggaagatga gactatcgag tcgatcgagc aaaccgccgt
     5881 gacccggaag gagcctccct gaatctttca ccgcaaaagc acgcttgatc catgactcaa
     5941 acaaatcgcc gatttgaccc aagacgctaa gcagcgcaga taagatcaca agctcaaccc
     6001 agcctagtcc catggcaagg gaaatcccca ccaagtaacc ggccaaaaca ctcccgagta
     6061 cgtatccaag agctccctca accgttttcc cggggctgat ttcgggcgcc agttttttgg
     6121 ttccgaagcg acgcccgacg aagtaggcgc agatgtcgcc tgccataatc accacgagaa
     6181 cgaagaacac ccaagctctt ccatgcggca gcctaaacag cgaactccaa tgtggcaaaa
     6241 gatagccgag gtacagtgat cccaaaagaa tccaggcaag tcgcgccagt cgctcctcga
     6301 gttgcccttg catgaaaatg tagatcgaaa acatcaggac aagcaatgga gctaggatta
     6361 tctctccagg cgttagctgg ggaaaaaaga ctgccgaaga aacggcaagg ccgaaagcta
     6421 cgccgattgt ttgctctcta ggacgtccag gaaaacccat ggcaaaatac tcacgcaagg
     6481 ctccaaacgt aatcagcaaa aaaaagccgt gaaacaacca gggatcaccc caaccaatga
     6541 gaacaactaa cagcggtatt ccgacaagcc ctgtaagcag ccttgcccta agattcgcgt
     6601 ccactactcc ccactctcag ccgagccggt tcgctaatct gctcgtcggt tctcccgaat
     6661 cgcctgcgac ggcgttgata ctcaaatagt gcttgtaggt attcttgttc acggaagtca
     6721 ggccaaagag tcggagtcac atagagttca gtataagcca tctgccataa gtaaaagttg
     6781 ctgattcgca tttcaccact ggttcgtatc aagagatccg ggtcgggtat gtcgggcgta
     6841 tccatatgag cagaaacaat ctcctcctcg acatcgctcg attggaattc gccggcgaga
     6901 atcttgcgcc taatcttgtt aaccattcgc acgatctcat cacgtccgct ataactcagg
     6961 gccaaaacca cggtcatccg actattgcgc tcggttaact ctatcacctc ctccagcgtc
     7021 ctccggacat ttagcggcag tcgatcccgg tttccgatcg cctttaacct gatgccgtaa
     7081 cgcatcattt ttggctgttc agcaatgaga taggtttcga gcaagcccat caatgcgtga
     7141 acttcatcgt gcggacggag ccagttttcc gtggaaaacg cgaagagagt caagaacggg
     7201 atacccagct tgcgcgtcat ttcgacaata acgcgaacgg aggtttttcc ccggcggtgc
     7261 ccttcgatgc ggctctttcc ctgcagagtc gcccagcgcc cgttcccgtc catgatgatc
     7321 gcgacatggc gtggcagccg acttctatca agatccttag aatccattgt tgagccgcgg
     7381 gtgcgtgaag tgatgggaca gtgggcgccc cgaatttgaa tggaacgccg cgcatgtagg
     7441 ttggctgttg cggagatctc cggtccctag acctccatga tctcctcctc ttttgttttt
     7501 aaaaccttgt ccacccgggc gatgtaatca tcggtgattt tttgaactcg ttcctgaccg
     7561 tgtttgaatt catcagcggt aatctctttc tttttttcgg attcttttaa atgctcgatg
     7621 gccaagcgtc ggtgattacg cattgagacg cgaaactctt cgccggtttt cttgacgtgc
     7681 tttaccaaat ccttacggcg ctcttcagtc agctgtggta tggggactcg gatgatcttg
     7741 ccgtcattgg cgggcgtcag gcccaagtcg ctcttaagaa tcgctttttc aatttcagct
     7801 agtgcgtttc ggtcgaaagg ctgaatcact aagagccttg gctccggagc acttaatgta
     7861 gccaattgat tcaaaggagt agtggcgccg taataatcaa ccagaacgcc ttccaaaaga
     7921 gccgtcgatg ctctccccgt ccgaaccctt cccatttctt tgcgcagagc ggcgacagac
     7981 ccttccatct ctttttgcaa ctgagtgaaa atagcctcgt tcatcgtttc cccgcactta
     8041 ctaatgtgcc gattgcttca ccggccacga ctctttttat gtttcccttt tccattaaat
     8101 tgaaaacgac aatgggaagc tgatgatcca tgcagagtga gattgccgtc gaatccataa
     8161 cctttaactc acggtttaaa acctcgatgt aagtcagctg ttcaaacttc gtcgcccctt
     8221 cgaatttgaa cgggtcagcg tcataaacgc cttccacctt cgtcgccttc aatatgactt
     8281 cggcgccaat ctccatcgct cgcaggctcg ctgtagtgtc ggttgtaaaa tagggattgc
     8341 cggtaccgcc ggcaaaaatg accacgcgcc ctttttccag atgccgtgtt gccctacgcc
     8401 tgatgtaagg ctctgcaacc tgcctcattt cgattgctga caacacacgg gtggaaacac
     8461 ccaacttttc caaggcatct tgtagcgcaa ggctgttgat aactgttgcc agcataccca
     8521 tagtatcggc gctcgcccga tccatgccga tctcgctcgc gaccgcgccg cgaaatatat
     8581 tgccaccacc tacgaccaac gccatctcgc agccaatctc tttgacttct ttgatctcct
     8641 gcgcaaacgc cttgaccgtt tgcgcatcga taccaaactt ctgcgcgccg gcgaaaattt
     8701 ctccggtgac tttcagaatg atgcgtttgt actttagatt cgcctcgctc atgcgtttct
     8761 tccgacaaac cttactcctg tggtgctcct tcgcccaatt gatatcgcga gaagcgcttc
     8821 acctgaatgt tctccccaag acgggctacc gcgtcggtaa ggatctcaga aacttttcgg
     8881 tcggggtttt tgataaacga ttgctcaagc agacaaactt ctgaataaaa tcgatcgatc
     8941 tttccatcga cgatcttatc gacgaccttc tccggcttac ctgactccag agcttgacgg
     9001 cgatatatct ccctttcctt ttccagatct ccggtcggaa cctcttccgg tcggatgtat
     9061 cgcggattag ccgcagcaat ctgcatggca atatctttta agagattctg aaactcggga
     9121 gtgcgcgcga caaagtcggt ttcgcagttg agctccacca ggacgccaat tttaccgccc
     9181 ggatgcacat acgcgccgat ggcaccgtcg ctggctgctc gagacgcctt tttgctggcc
     9241 gctgcaagcc ccttctgacg caaataatca accgctttgt ccagatcgcc gccggattcg
     9301 gctaaagctt tcttgcaatc catcataccg gcgcccgtct tttcacgaag attttttacc
     9361 gcagccgcac tcacttccat cgactaaact cctcccatgg ccgactgttc taccggatgc
     9421 tccatctcag tcggggccgg tacggcagac gatccttcat ctcccttgtc tttggcgaga
     9481 gactgatcat agaggccctt accctcaatt actgcatcgg ccatggacga gcaaaataac
     9541 cggatggctc tgatcgcatc atcatttcct gggattttgt gatctaccat gtcgggatcg
     9601 caattcgtgt cgatcacggc gatcacaggg ataccaagct ttcttgcctc ctttacggca
     9661 atttcttcct gcttgggatc aaccacaaaa atcgcatcgg gtagcttacg catcgccttg
     9721 attcctccaa gcgactgctc gagcttgtcc ttctcccgct ttaagccaag catttccttt
     9781 ttggtcaaag cgtcgacaat tttaggatca ttttccattt cttctagttt tctcaggcga
     9841 tcgatgctct ggcgaatggt ggagaaattt gtaagagttc cgcctagcca gcggt
//
LOCUS       DASUTG010000750         8818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_558529, whole genome shotgun sequence.
ACCESSION   DASUTG010000750 DASUTG010000000
VERSION     DASUTG010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558529"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..2250
                     /locus_tag="VF089_17365"
     CDS             202..2250
                     /locus_tag="VF089_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6175784.1"
                     /translation="MILDAALEALANLAVPMRLLFLLFGVLMGLALGAMPGLGGLVGL
                     AILLPFTFDMDPFMAFSIMIGLISVTSTSDTIPSVLFGVPGTAAAQATIMDGHPMAKN
                     GEAGRAFGAAYTASLMGGLFGALILAVSIPVIRPVVLAFGSPEFFMMGMLGISMVAVL
                     SGNAPMKGLIAGAFGLAIGMVGMDPQTGVIRWAFGALYLWDGIPLVPVALGLFAIPEV
                     VDLVIKGTSISNVPNQAISGVWLGIRDAFRHWFLVLRCSAIGVWVGATPGLGASVVDW
                     FAYGHAIQSEKGASKSFGKGDVRGVIAPETANNAKEGGALIPTLAFGIPGSASMALLL
                     GAMTIQGLSPGSAMLTKHLDVTFTMIWSLVIANVFGTVICLLFTNKLARVAAIRIHLI
                     APIVIGIVFVASFQATSHYGDLWSLLAFSLLGWFMKRFSWPRPPVILGLVLSKIIENY
                     LFISVTRYEWSWLTRPIVIGIGLLIVVSLFFGFRGSMNNRKSSETGSKAPLTFKFNPG
                     SILTLLFLAAFTFGVATAGEWDIHARLFPWVIGIPAIFLCLGQLFGDFFGRRNLAGEE
                     DTRGMMDLPVDRSVPMSVVVARAVKTFVWITGLFVAIYLAGLVIAVPLFVLFYLKFQG
                     DEKLWLSLKSAAAMALLLVGVFHLVLNVPWPEAVFPQAEEVILERVESFYERLWSAAL
                     "
     gene            2303..2770
                     /locus_tag="VF089_17370"
     CDS             2303..2770
                     /locus_tag="VF089_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175785.1"
                     /translation="MKLMQNFSKNRLLSLGALFVTTALGGSSLVGGCALGGSGDSERL
                     RTSPKLTVSPSPVPYERKTKVNIAGSGFAPNQELELQIPIGGVLTDISGMVKPAPKTN
                     DQGAFSSQWILDNEIRGKLMEPTEYTLEVLNSDGKVLAKVPFVLEKVEAKKKN"
     gene            2818..3930
                     /locus_tag="VF089_17375"
     CDS             2818..3930
                     /locus_tag="VF089_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175786.1"
                     /translation="MRWSVSSCRSLTALAAIAVLALSLIAVPSAARGASSFEGERIKI
                     LVGFPPAGGHDLEARLIARYLPKYLPGKPSIIVQNMPGAGGMIMGAYIYNRTKPDGTS
                     IGLFGGSHLQAGIIGTGVEYDIAKMPIIWGVRGVRIGIVRDFLKADSAKELAQIDASK
                     IAVAGRSRTDSSCVMGSMAMTLLGIKDYKTVCAYAGTAVIRGAIERGEASYFDASDAH
                     LVGGGAFVELYKKKQVIPLWQAGKLTDDGKIVRSSTVPKGVPTFEEAYQQARGKAPAG
                     PLWDAFRTLYNSVHGTLNRVLVLPPGTPQDTVQVLRSSLARMADDKAFVRDWEKIFGQ
                     EFSESRVSAKEAEKVKDEFSKPAPWQDELRKFLNVS"
     gene            4240..4401
                     /locus_tag="VF089_17380"
     CDS             4240..4401
                     /locus_tag="VF089_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175787.1"
                     /translation="MNAWQSTCPRHTIWEDAIIDGLIQLNGAGTCHDPEVALTPRGKA
                     YLDGNTRNQ"
     gene            4858..5397
                     /locus_tag="VF089_17385"
     CDS             4858..5397
                     /locus_tag="VF089_17385"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6175788.1"
                     /translation="MSRLPVIDREALAPADQAVWDRIAAVRTGVRGPFGVLMHVPALA
                     DRIAALEDYFRFNAALPAVDRELVIMAAAREMNARYPWARHEARGREVGTRTEAIEAV
                     RLNGPLKQLTTRERLLVEIVRTLLRTHVLPEALYTQALAELGRQQLIEAVALAGHYSL
                     IGLTVNAFDVAPPDNGSTF"
     gene            5639..6658
                     /locus_tag="VF089_17390"
     CDS             5639..6658
                     /locus_tag="VF089_17390"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX6175789.1"
                     /translation="MKTDDLRIVKTRPLLAPAILAEEIPLTDTAAARVHDTRRAIEAI
                     LDRKDTRLLVVVGPCSVHDTKAALEYATKLKPVAAALSHALLLVMRVYFEKPRTVIGW
                     KGLINDPDLDESYRINKGLRLARQLLADILDLGIPAGTEFLDTTFGQFYADLISWGAI
                     GARTAESQIHRELASGLSMPVGIKNRTDGNVQIAVDAIQTARSRHLFPSLTKEGAPAI
                     LETTGNPYAHLVLRGGSETGPNFDADSIESAVRMLRAAGLPEVVMVDCSHGNSEKDAA
                     RQIDVADEIMENLRGSPIRALMIESHLVAGRQNAPITYGQSITDACLGFEKTKELLYR
                     LADAV"
     gene            6954..7955
                     /locus_tag="VF089_17395"
     CDS             6954..7955
                     /locus_tag="VF089_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX6175790.1"
                     /translation="MTVRIAQFVHATHDVVDEAFLREFPEIEVVKANNIETLSDALEG
                     AEILHIYNSAFTPEFAQLVRDKGRALKWVQFTTVGIDIALKAGLPDGVWVTNSGDVSQ
                     RVLAGHAIALMLGVMRGFHRFEPLRAQRNYARQIMPRYMMAPDGARMMILGMGRIGQD
                     IARKAKAFDMEVICVTRAAAPAVPEIDRVVPREKVEEVLPTADVIMNAMPLDAGTQGF
                     FSAERIALMKETAVFVNISRGKVVDEAELARALAEGRIMGAGLDAFAREPLPVESPLW
                     DLPNVLITPHVGGNGGRELWRRMSELVRDNTRRYLSGAPLKHVVRTPNGTLQGFGNS"
     gene            8040..8702
                     /locus_tag="VF089_17400"
     CDS             8040..8702
                     /locus_tag="VF089_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX6175791.1"
                     /translation="MSDESYNLIKNDFIMALRIIYREGLSDAFAHLSLRSNEGNEMMF
                     MPRKSPALVTSDELFFVDFEKPVPQSSLHQAIYKARSDVKAILHFHSPAVILLSVVGQ
                     TIRPMHNYSAIFYEGVPLYTGTGQVESPARAGEMTKLLGNAKALMLRGHGAVVVGQSI
                     REVCMLALFLEESARLQTEAMKLGTPMFMERDEAEKIARRTFKPTSVERAWDHFVAKH
                     AD"
BASE COUNT         1908 a         2339 c         2508 g         2063 t
ORIGIN      
        1 aaggtgattc gacaagaaaa ttccgcctcg tctgacctgg tctgccgctc tgctgaggag
       61 catctaaata cgactagtta cctacgacct gcttgactta gcactggttt gctagatagt
      121 tgttcctgtt gttaggtctg aagtcgtacg catgtgtcgc aaaggacaat agcctacccg
      181 tagttcaggt cggcaaagct catgatactt gatgccgctc tagaggcttt ggcgaatctg
      241 gcggtcccca tgcgactgct ttttctcttg tttggcgtcc tcatgggtct ggcattgggc
      301 gccatgcccg gtctcggcgg cctcgtgggg cttgcgatcc tgttgccgtt tacgttcgac
      361 atggatccgt tcatggcatt ttcgatcatg atcggcttga tctctgtcac gagcacttcc
      421 gataccattc cctccgtgct ctttggcgtg ccgggtacgg ccgcggctca ggcaacaatc
      481 atggacggtc atccgatggc gaagaacggc gaagccggtc gcgcctttgg cgccgcctat
      541 accgcatctt tgatgggcgg ccttttcggc gcacttattc ttgctgtttc gataccggtg
      601 attcgcccgg tggtcttggc gttcggatct ccggaatttt ttatgatggg aatgctgggg
      661 atttccatgg ttgcggtgct gagcggcaat gcgcctatga agggacttat tgccggagca
      721 tttggcttgg ccatcgggat ggtgggcatg gaccctcaga cgggagttat tcgctgggcg
      781 tttggtgcgc tttatctctg ggacggaatt ccgctggtgc ccgtggcact cggactgttt
      841 gcaatacccg aggtcgtcga cctggtgatc aagggaacca gcatatccaa tgtgccgaat
      901 caagccatca gcggcgtatg gctgggtatc cgagacgcct ttcgtcactg gtttttagtc
      961 ttacgatgca gcgccatcgg tgtttgggtt ggtgccactc ccggcctcgg cgcctcggtt
     1021 gtcgattggt ttgcttacgg ccacgctatt cagtccgaaa aaggcgcatc caaatccttc
     1081 ggcaaaggcg atgtccgcgg cgtgatcgcg ccggagaccg cgaacaatgc aaaagaaggc
     1141 ggcgcgctca tcccaaccct ggcgttcggg attccgggaa gtgcgtccat ggcgctcttg
     1201 ctcggcgcca tgacgattca aggactgtcg ccgggctcgg caatgctgac aaagcatctg
     1261 gacgtgactt ttacgatgat ttggagcttg gtgatcgcca atgtttttgg cacggtaatc
     1321 tgcctcctat tcaccaataa actggcgcgc gtcgcagcaa tcagaattca tctgatcgcg
     1381 cccatcgtca tcggtatcgt cttcgtcgca tcctttcagg ccaccagcca ttacggcgat
     1441 ctttggtcgt tgttggcctt ttcactgctc ggttggttta tgaaacgctt cagctggccc
     1501 cggccgccgg tcattctcgg cctcgtgctc agcaaaatca tcgaaaatta tctgttcatt
     1561 tcggtaacac gatacgaatg gagttggttg acccggccga tcgtcatcgg catcggttta
     1621 ttaatcgtcg tcagtctctt cttcggtttt cgcggcagca tgaacaatcg taagtcaagc
     1681 gaaacgggca gcaaagcgcc gctaacattt aagtttaatc ccggctcaat cttgaccttg
     1741 ttgtttctcg ctgcgtttac tttcggcgtc gccaccgccg gtgaatggga cattcacgcg
     1801 cgcctgttcc cgtgggttat aggtattcca gcgattttct tgtgcctcgg gcaactcttc
     1861 ggtgattttt tcgggcgcag gaacctcgcc ggcgaggaag acacacgcgg catgatggat
     1921 cttcccgtcg accgcagcgt tcccatgtcc gtagttgtcg cccgtgctgt caagacgttt
     1981 gtttggatca ccggattatt tgtcgcgatt tatctcgcag ggcttgtcat cgccgtgcca
     2041 ctcttcgtgc ttttctatct aaaatttcaa ggtgatgaaa agctttggct atctttgaag
     2101 tccgcagctg cgatggcttt gttgctcgtc ggcgtatttc atctggtgtt gaacgtgccg
     2161 tggccggaag cggtatttcc acaggcggaa gaggtaattt tagaaagggt cgaaagcttt
     2221 tatgaacggc tttggtcagc ggctctatag ctactaatac ttttcggcag agtgaatttt
     2281 tatcagcaga aaggaggctt aagtgaagtt gatgcagaat ttttcgaaaa ataggctgct
     2341 ttcccttggc gccctgttcg tcacgacggc gctaggagga agcagtttgg tcggtggttg
     2401 tgcactggga ggttcaggag attccgagag actcaggacc agtcccaaac tgacggtttc
     2461 gccttcgccg gtgccgtacg agcgcaaaac taaagtaaac atagccggtt ccgggtttgc
     2521 gcccaatcag gaactcgagc tgcagatacc gatcggcggc gtgctgacgg atatcagcgg
     2581 gatggttaaa cctgcaccca agaccaacga tcagggggcg ttcagcagcc agtggatcct
     2641 cgacaacgag attcgtggga aactcatgga gccgaccgag tacactctgg aagtgttgaa
     2701 cagcgacggc aaggttctgg cgaaagttcc cttcgtcctg gagaaagttg aagcgaagaa
     2761 aaaaaactaa tccacgagga gactttccgt cgactcgtga attaaggagg aaatgttatg
     2821 cgatggagcg tatcaagttg tagatccctg acggcattgg cagcgatcgc ggttctcgcc
     2881 ttgtctctga tagcagtgcc cagcgcggcg cggggggcat cgagcttcga aggcgaacgg
     2941 ataaaaattt tggtaggttt tccaccggcg ggcggccatg acctagaggc gagactcatt
     3001 gcccgttatt tacccaagta cctgccgggt aagccatcca ttatcgtcca gaacatgccg
     3061 ggggcgggcg gtatgatcat gggagcctac atttacaacc gtaccaaacc ggatgggacg
     3121 agcatcggac tatttggcgg atcgcatctt caggccggca ttatcggcac aggtgtcgaa
     3181 tacgacatcg cgaaaatgcc aatcatctgg ggtgtacgag gagttcggat cggcatcgtg
     3241 cgtgattttc tgaaggccga ttcggctaag gaattggcgc aaatcgatgc ctccaagatc
     3301 gccgtggccg gtcgttccag aaccgactcg tcatgtgtca tgggcagcat ggccatgact
     3361 ctgctcggca tcaaagacta caaaacagtt tgcgcttatg caggaacggc tgttattcgg
     3421 ggtgcaatcg aacgaggaga agcgagttat tttgacgcca gcgatgcgca cctggtgggc
     3481 ggcggcgctt ttgtcgagct ctacaagaag aagcaagtca tccccctctg gcaagccggc
     3541 aagctgaccg atgacggtaa gatcgttcgc tcatcgacgg taccgaaagg tgttccgacc
     3601 tttgaagaag cttaccagca agcgcgcggc aaagcgccgg ccgggccgct ctgggacgcc
     3661 tttcgcactc tctacaacag cgtacacggc acattgaatc gagtcttggt tttgcctccg
     3721 ggaacgccgc aggataccgt tcaagtgctg cgatcgtcgc ttgcccgtat ggccgacgat
     3781 aaggcttttg tacgtgattg ggaaaaaatc ttcggccaag aattctcgga atcgcgcgtc
     3841 tccgccaaag aggctgaaaa agtcaaagat gaattcagta agccggctcc ttggcaggat
     3901 gagttaagaa agtttctcaa cgtttcctga cccctcaata ttgccgcggc gatggacgta
     3961 tcgccgcggc acatttcctc agtgctctga taccatctcg attatcgacg ttgggcttct
     4021 tagttctcta cgcaacaacc ctaatctgca aatttctgtg ccaaatcaca gctcgtcctt
     4081 acgcttgaac tttgatccgc aacgcataga atagttgcgt ttccggagcc ataacgatcg
     4141 tgctgtacga tggagtctat gatgccagag tcggcgaacc tcttgatgct ggagttcctt
     4201 tcctgggtct caagtcgccg tcggacttac gcggaggcta tgaatgcatg gcaaagcacc
     4261 tgtccgagac ataccatttg ggaggatgcg atcatcgatg gcctcattca actaaacggc
     4321 gccggaacat gccacgatcc tgaggttgcg ctcacgcctc gcggcaaagc ttatcttgac
     4381 gggaacaccc gtaatcagtg acctctcgat ccgtcatttt tttatatgat ctatacaatg
     4441 caacagtccc ggtttagcag agtggtttca actggtttgc gggcgccctg tatggagaag
     4501 catcaccagt gaacaataga cttgattgac ggcatcccat ctatgaaaaa cttttgataa
     4561 ttcgcacctg ctgtagtaac taagctgtcg accaacgcgt tacccggagg aaagtaagga
     4621 ccattaagac gatactttcg attatttgcc tgctttggat ctccactacc gcggccgccc
     4681 agtctcccaa agcaactgta ttggtcgacc ttgatcccac agctcagcgc aaacggtaat
     4741 tgttgagagc gtcgccgagg gttcgaaaat gcaagaggcg ctgaaagaga aggatgaaag
     4801 cgaaaatttc gtttcagctt tcaaatttaa tactttgtaa ctcgatgaga ggaaaccatg
     4861 agccgattac cagttattga ccgcgaagcg ctggcgccgg ccgatcaagc ggtctgggac
     4921 cgcattgctg ctgtccgtac gggagtgcgt ggaccctttg gagtattgat gcacgtccca
     4981 gcactcgccg accgcatcgc agccctggaa gactattttc gttttaacgc agcgctgccg
     5041 gcagtcgacc gtgagctcgt gatcatggct gcggcgcgcg aaatgaacgc gcgttaccca
     5101 tgggcgcggc acgaggccag aggacgtgaa gtgggcacaa gaaccgaggc gatcgaagcg
     5161 gtgcgcctta acggcccatt gaagcagcta acgacccgcg agcgtctact cgtggaaatc
     5221 gtgcgcaccc tgctgcgcac gcacgtatta ccggaggcct tgtatacgca agcgctggcc
     5281 gaactggggc ggcaacaact gatcgaggca gttgcgctcg caggccatta cagtctgatc
     5341 gggttgacgg tgaatgcatt cgatgtcgcg ccaccggata acggttcgac tttctgaact
     5401 gcgcgccagc tcctcattac tagacacctg ctccgttgcg cttgctctcc caaagctttc
     5461 gaccacctgt gaagcaaacg caattcatca tacgcggttt gacactattt ccgatagtcg
     5521 agtactcttt ccaaaatgtg tagtagctgt tcaatcgatt tcgatcagct tcttaggtcg
     5581 gccgaggcga ctagcttatg cgacaaatct cgggtttttt ccagccggca attgagccat
     5641 gaagactgac gatctgcgca tcgtcaaaac ccggccactg ctcgcgccgg cgatactcgc
     5701 cgaagagatc cctctcaccg acaccgcggc agcacgagta catgatacgc gccgagccat
     5761 cgaggcgatt ctagatcgca aggatacgcg gctcctggtt gtcgtcggtc cttgctccgt
     5821 gcacgacacc aaagccgcgc tcgagtatgc aaccaagctc aagcccgttg ccgctgcgtt
     5881 atcccatgcg cttctccttg tcatgcgggt gtacttcgaa aagccgcgaa cggtcattgg
     5941 ctggaagggg ctcatcaacg atccggacct cgacgagtcg tatcggataa acaagggctt
     6001 gcgactcgcc cggcaattgc tcgccgatat tttggattta ggaatcccgg cgggaacaga
     6061 gtttctcgat accacatttg gccagttcta tgccgatctc attagctggg gtgcgattgg
     6121 agcccgcacc gcggagagcc aaatacatcg agagctggca tcgggattgt cgatgcctgt
     6181 ggggattaag aatcgaacag acggaaatgt tcagatcgca gtggatgcga tacagaccgc
     6241 gcgcagccgg catttgtttc cgtcactcac caaagaaggc gcgccggcga ttctggaaac
     6301 caccggcaat ccatacgcgc atctcgtcct ccggggaggt agtgagaccg gacccaactt
     6361 tgatgctgat tctatcgaga gcgccgtgcg aatgcttcgc gcagcgggtc ttccagaggt
     6421 cgttatggtt gattgcagcc atggcaacag cgaaaaggat gcggcacggc agattgatgt
     6481 ggcggacgag atcatggaaa acctgagagg ttcaccaatt cgagcgctga tgattgagag
     6541 ccatctcgtc gctggacgtc agaatgcgcc cataacgtac ggtcagagca tcaccgatgc
     6601 atgtctgggg ttcgagaaaa cgaaagaatt attataccgg ctcgcagacg ccgtttaacg
     6661 gcccgtcacg aaagtcactc gctaccaacg ggttctcgac ctctacgcct cgggctcttt
     6721 tgtcgatcac tgaagaccaa ctcgctgctg atgaccggct gcgcgatgtt cgtgttgggg
     6781 cgaaagtcag gttaggatcg cggtctcgcc gcgggtaatg tcgcttgtga atcttatcat
     6841 ccgcaaagat cagaatgaaa aatttgagat aacatcgaaa gattcgacac gaacagctac
     6901 tataataaaa gacggaagag ctatttccga taccgaaatc gattagagca gctatgacag
     6961 ttcgcatcgc gcaattcgta cacgctaccc acgatgtggt cgacgaagct ttcctgcggg
     7021 aatttccaga aatcgaagtt gttaaggcga acaacatcga aaccctgtcc gatgcgctcg
     7081 agggagcgga aatcctccat atttataatt ccgcctttac gccggagttc gcccagctcg
     7141 tgcgcgacaa ggggcgggcg ctcaagtggg tccagttcac cacggtgggg atcgatatcg
     7201 cgctcaaagc ggggctgccc gatggtgttt gggtcaccaa cagcggcgac gtcagccagc
     7261 gggtgctggc gggtcacgcc atcgctctaa tgctcggggt catgcgcgga tttcatcgct
     7321 tcgagccgct gcgcgcgcaa cgtaattatg cgcggcaaat catgccgcgt tacatgatgg
     7381 cgccggatgg tgcgcgcatg atgattctcg gcatgggccg catcggccag gacatcgcgc
     7441 gcaaggccaa ggcattcgac atggaagtga tctgcgtcac tcgagcggca gctcccgcgg
     7501 tgcccgagat tgaccgggtg gtgccccgtg aaaaggtcga ggaggtgctt cccaccgccg
     7561 acgtaataat gaacgcgatg ccgctcgatg ccggtaccca gggatttttc agtgccgaaa
     7621 gaatcgcgct catgaaagag accgcagtgt tcgtgaacat ctcccgcggc aaagtggtgg
     7681 acgaggctga actggcccgc gcgctggccg aaggtcgtat catgggcgca gggctcgacg
     7741 cattcgcccg agagccgctg cctgtggaaa gcccgctgtg ggatctcccc aacgtcctga
     7801 taacgccgca tgtgggcggc aacggcgggc gcgaattgtg gcggcgaatg agcgagctcg
     7861 tgcgcgataa cacccggcgc tatctgtccg gtgcgccgct gaagcacgtt gtcagaacac
     7921 ccaatggcac gctgcaaggc tttggaaatt cgtaggctgg caactcatag caaatgattc
     7981 tgcgggtgtg cctgagtatt gaattactct gcccgcccga cgggtaggaa aagacgacta
     8041 tgtccgatga gtcttacaat ttgatcaaaa acgatttcat catggcactg cggatcattt
     8101 atcgcgaggg actgagcgac gcatttgctc atctcagcct gcgcagcaac gaggggaatg
     8161 agatgatgtt catgccgcgc aagagtccgg ctttggtcac gagcgacgaa ttgttttttg
     8221 tcgatttcga aaagccggtg ccgcagtctt ccttgcatca agcgatttat aaagcgcgtt
     8281 ccgatgtcaa agccatcctt cattttcatt cgccggcggt gattctgttg agcgtcgtgg
     8341 gacaaaccat tcggccgatg cacaactaca gcgcgatttt ctacgaaggg gtgccgctct
     8401 ataccggaac cgggcaggtg gaatcgccgg cgcgggcggg tgagatgacc aagttgctgg
     8461 gcaacgctaa ggcgctgatg ctgcgcgggc atggcgcggt cgtcgtcggt caatcgattc
     8521 gcgaagtctg catgctggcg cttttcctgg aggagtcggc gcggctgcaa accgaagcca
     8581 tgaaactcgg tacgccgatg ttcatggagc gcgacgaagc cgagaagatc gccaggcgca
     8641 ctttcaagcc gacttccgtc gagcgcgctt gggatcactt tgtcgcgaaa cacgccgatt
     8701 gattttgctg ctcctgctgt ttgcccgatc ttcaatcgcc gcttctcgcc gcgtaaactt
     8761 tgacaggttg agagctgcgt ttcgcgtgga ctgtttgttc tcgttaactg taaatccg
//
LOCUS       DASUTG010000751         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2041149, whole genome shotgun sequence.
ACCESSION   DASUTG010000751 DASUTG010000000
VERSION     DASUTG010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041149"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1004)
                     /locus_tag="VF089_17405"
     CDS             complement(57..1004)
                     /locus_tag="VF089_17405"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6175792.1"
                     /translation="MTQKQKPRRPLLGAHMSIAGGVGNALLLGAKVQCDAIQIFTKSS
                     RQWACKPYSEEEIAQYQENQKTTGIKKVIAHDSYLLNLGSPDGTLRQRSIMAFIDEME
                     RCEILGISNLIAHPGSHLGSGEDQGIKTIGSCLNEVHQACPGYAVKVTLEITAGQGSN
                     LGYRFEQIVKMFDATRESERLRVCFDTEHAFAAGYDIRTREGYERTFTEFDATIGIER
                     LAAFHLNDSKKEFNSRVDRHEHIGKGHIGVEAFRLLMNDKRFWDVPMCLETPKGLDLA
                     EDRENLGLLRSLFEPSSEIQSDAALLATTSERRQSSGPL"
     gene            complement(1108..2715)
                     /locus_tag="VF089_17410"
     CDS             complement(1108..2715)
                     /locus_tag="VF089_17410"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX6175793.1"
                     /translation="MPSFPDFCAEYFDKYFRLNPAEAIYYGVAGYDHLLNDYSEETYA
                     AQKRLVNDSLETLRQISAKDLDADQSIDYALLEGRLSIQKYEQEKEDYRLKWPDHYLA
                     TDAIYILTVRETDDLEKNLLSRLEGTANLVRQGIDNLSRPEANPPRLWTEMSIQAAQG
                     GITFLETLPSHPKVQATIKDGPTFRAAIDRAKSATRNYADFLIRDLLPRSRGLYAVGE
                     EHYNLLLRKKHFLDLNAQALLTMGENLFCRTRQELLALAEEFSPGRAINEVAQTIQAD
                     HPASEEILPAYQKAMESARQFVKEKNLVSFPPREELHVVHTPEFRRHEIPFAAYLSPS
                     PKDPNQVGYYYVTPVADEDLLREHNWVGLENTSVHESYPGHHLQFSIANSIPAASTLP
                     RLMNESSVFYEGWALYCEQLMQEQGFLKSRQHRFVMLKDRLWRALRIIIDVKTQTGAM
                     TYDEAADLMVRELHFPRAQACGDLNWYSQAPAVPMGYALGWSIITRMRQQETTRLGGK
                     FNLRDFHDRLLGAGSISLPLVERRHFT"
     gene            2920..3333
                     /locus_tag="VF089_17415"
     CDS             2920..3333
                     /locus_tag="VF089_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX6175794.1"
                     /translation="MPNRWLFKTEPSVYSFQQLQKDKKTVWDGVANNLALKNLKDIKK
                     GDLIFIYHTGDEKAAVGVAKALGGAYPDPSKNDPKLLVVDIEAGKPLARPVTLAEVKA
                     HPKLKNFDLVRNSRLSIMKVADEQWEIMEGMAKSQ"
BASE COUNT          832 a          918 c          954 g          824 t
ORIGIN      
        1 ccagggctga tacttttgcc ggattccggt gcactaaccg gcaagattgt agatctttac
       61 aacggaccgc tggattgcct tcgctccgac gtggtagcca gtagcgcggc gtcgctttga
      121 atctcggaac tcggctcaaa aaggctgcga agcaaaccta agttttcgcg gtcctcggcc
      181 aaatccagtc ctttgggtgt ctcgagacac atcggcacat cccaaaaccg tttatcgttc
      241 atcagcaatc gaaacgcctc cacgccgatg tggcctttgc cgatatgctc gtgtcggtcc
      301 acccgcgagt taaattcttt tttcgaatcg ttgagatgga acgcggcgag ccgttcgata
      361 ccgatggtcg cgtcgaattc cgtgaatgtg cgttcataac cctctcttgt tcgaatatcg
      421 tagcctgcgg caaaagcatg ctcagtatcg aagcacaccc gcaagcgctc gctttcccgg
      481 gtggcatcga acattttaac gatctgctcg aaacgataac cgagattcga tccctggccc
      541 gccgtgatct ccagggtcac tttcactgca taaccagggc atgcctggtg aacttcattg
      601 agacagctcc cgatggtctt aatgccttga tcttctcccg agccgagatg cgagccggga
      661 tgggcgatca gattcgaaat tcccaatatc tcgcagcgct ccatctcatc gatgaaggcc
      721 ataatcgaac gttgccgcaa tgtgccgtcg ggcgagccca gattcaatag ataagagtcg
      781 tgggcgatga ccttcttgat cccggttgtt ttctgattct cctgatactg tgcgatctct
      841 tcctcgctgt aaggcttaca cgcccattgg cgtgacgact tggtaaagat ctgaatcgca
      901 tcgcattgaa ctttcgcgcc gagcagtagg gcattgccaa cccctccggc aatcgacata
      961 tgggcgccga gcaacgggcg gcgtggtttt tgtttttgcg tcataacgtt ttgagcggct
     1021 tgaacgattt gaacttcttg aacgaattag ttgggccggc gccccagccg ttcccaaccg
     1081 ttccaaatgt tccagacatt tacaagatca cgtgaagtgt cttcgttcca cgagcggcag
     1141 ggaaatgctg ccagcgccga gcaggcgatc gtgaaaatcg cgtagattga acttgccgcc
     1201 gagtcgggtg gtttcttgct ggcgcatgcg agtgatgatc gaccaaccta aagcgtagcc
     1261 catcggaacc gcgggcgctt ggctgtacca attgagatca ccacaagcct gtgcccgcgg
     1321 gaaatgcagc tcgcgcacca tcaaatcggc cgcttcgtcg taagtcatcg ctccggtctg
     1381 cgttttgacg tcgataatga tgcgcaacgc gcgccatagc cgatccttga gcattacaaa
     1441 acgatgttgc ctgctcttta aaaaaccttg ctcttgcatg agttgctcgc agtacagggc
     1501 ccacccttca taaaaaaccg aagactcgtt catcagccgg ggcaaggtcg acgccgccgg
     1561 tatcgaattg gcaattgaga attgaagatg atgccccgga tagctttcat gaaccgaggt
     1621 gttctcgagg cccacccaat tgtgctcgcg cagcagatct tcatcggcga ccggtgtgac
     1681 gtagtagtag ccgacctggt tgggatcttt cggcgagggc gagagatacg cggcaaaggg
     1741 aatttcgtgg cggcgaaact ccggcgtgtg gaccacgtgc agttcttccc gcgggggaaa
     1801 agaaacgagg tttttttcct tgacgaattg ccgggccgac tccatggctt tctgataggc
     1861 cggaaggatt tcctcactgg caggatggtc agcctgaatc gtctgcgcca cctcgttgat
     1921 ggctctacct ggtgagaact cctcggcgag cgcgagaagt tcctggcgcg tccggcagaa
     1981 aaggttttct cccatagtga gaagcgcctg cgcgttcaga tcgagaaagt gcttttttct
     2041 gagcagcaag ttgtaatgct cttccccgac tgcataaagg ccgcggcttc ggggcagaag
     2101 gtcgcggatc agaaaatcgg cataattacg agttgcgctc tttgcccggt caatcgccgc
     2161 tcgaaacgtt ggaccatcct tgatggttgc ctgaaccttt ggatggcttg gcagcgtttc
     2221 cagaaaagta atgccgcctt gggcagcttg aatagacatt tcggtccaga gccgaggcgg
     2281 attggcttcc ggccgactga gattgtcgat tccctggcgg accaaattgg ccgtgccttc
     2341 caagcggctc aaaagatttt tttccagatc atcggtctcc cggacggtca ggatgtagat
     2401 cgcgtctgta gcaagataat gatcgggcca cttaagccgg taatcctctt tctcctgctc
     2461 atacttttgg atactcaagc gcccttcaag gagagcgtaa tcaatgctct gatccgcatc
     2521 caaatccttc gccgatatct gccgcaaggt ctccagcgaa tcattaacca gcctcttctg
     2581 cgcggcataa gtctcttcgg agtaatcgtt caatagatga tcgtacccgg caacgccgta
     2641 atagatcgct tccgcgggat taagacggaa atatttgtcg aaatactcag cgcagaaatc
     2701 ggggaaacta ggcatggatc tggattacca tcttaatgga tgcggcgcaa ttttcgttcg
     2761 tatgggcgga tgtgactccg tgtccgcagt gagcatagct gtcaagctac gtcgcgatga
     2821 gccgttgccg cgattacgcg cgctctctct cgaatgtcat gcgcatgttt gtattgaagg
     2881 catctgctgt tctatataaa agcatgcagg aggagttcca tgccaaaccg ctggttgttc
     2941 aagacagagc caagtgttta ttcttttcaa caattgcaga aagacaagaa aacggtatgg
     3001 gatggggtgg ccaacaattt agcgttgaag aacctcaaag acatcaaaaa gggcgatttg
     3061 attttcatct atcataccgg tgatgaaaag gcggccgttg gtgtggcaaa agccttgggc
     3121 ggcgcctatc cggatcccag taaaaacgat ccaaagctgc tggtggtcga tatcgaagca
     3181 gggaagccgc tagcgagacc cgtcactcta gccgaggtga aagcgcatcc gaagttaaag
     3241 aatttcgatc tggtgcgaaa ctcccgttta tcgatcatga aggtggcgga tgagcagtgg
     3301 gagatcatgg aaggaatggc caagagccaa taggcagtag gcgacaaaca ccagtaacac
     3361 ggcaaaatag ttcgactcgg cgaactctgc gtctctgcgg tcaatgatcg gattttcaac
     3421 gcagaggcgc gaaggacgca gagggtatgc aaagtaagaa caaggatggg aaaatggcgg
     3481 aacaaggcaa acacaatcac cgacacgacc acgatcatca acacggca
//
LOCUS       DASUTG010000752         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1005553, whole genome shotgun sequence.
ACCESSION   DASUTG010000752 DASUTG010000000
VERSION     DASUTG010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005553"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VF089_17420"
     CDS             <1..144
                     /locus_tag="VF089_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX6175795.1"
                     /translation="YLKTLSEEANESFDENLTKAQASKRIEELQQKTGRGAGASKQTG
                     ADK"
     gene            complement(552..1640)
                     /locus_tag="VF089_17425"
     CDS             complement(552..1640)
                     /locus_tag="VF089_17425"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175796.1"
                     /translation="MRIIDADTHIDETDATWEYFGDAELSFKPSTTYPANPDPKLPPA
                     RYWMIDGKRQVRFIRSDVDSGTTVETRELLDVKARLKHMDELGTDIQVIYPTLFLMEA
                     SEKPELNTAMRRSYNRWLGDRCGESNGRLRWVCMPPLQNMEAALEEMHWAKEHGACGV
                     LKKGDREGGKYPADPYFEPFYAEAEKLDLPICIHTGSGVPDFSPAREFSHGQFLRTKG
                     AAINGVLGLILHNIPAKFPKLRIGCIEAGSMWAPFVAYDLRRQQEKQKGRTALGVLGV
                     PQVELAENVFKANRVYITCQVDEDLPYIIQCVGEDNLMVGSDYTHRDPSMELEFRKIL
                     QERADKGDIPQSAVQKILYDNPKTFYAL"
     gene            2063..3103
                     /locus_tag="VF089_17430"
     CDS             2063..3103
                     /locus_tag="VF089_17430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175797.1"
                     /translation="MNDVPVIDADGHVLESDSELAQYFEGAYQGHKRSGVFSIFPSLD
                     GWPRGFVKGMDKVSATSAEAWIEFADEARLQAAVLYPTAGLAFGLIQDPDWACVVARA
                     YNNWLTDRYCKADSRLKGVAMLPVHNPQEAAAELRHAVLEQNMVAGMLPAVTMLNKGY
                     GHADFHPIYQEAQKLNVPLAVHGAVSRGLGFDFLQTMSMIHTLEHPIAQMIQLTSVVL
                     DGVFDLYPKLRMGFLEAGAGWIPYMMDRMDEKFHIDRKRKHFPLSKPPSEYFKSGNVY
                     VTCETDEKILDAVIREMGEDFIMYPTDFPHERQAGEFATDIPKFLKRPDLSESAKRKI
                     LSENAKRFYGIE"
BASE COUNT          721 a          866 c          842 g          732 t
ORIGIN      
        1 tacctaaaaa cgctttcaga agaagcaaac gagtcgttcg acgagaatct caccaaagcg
       61 caagcgtcca agcgtatcga agaactacag caaaagactg gacgaggtgc tggtgcgagc
      121 aagcaaaccg gcgccgataa atagcttcag gtgccgctcg accgcaaact tcgcaggcta
      181 gttgctggca ctagtctgaa gtccgactga gttgtagaaa cttcgcagta tgctgcggcc
      241 tgggattccc agaagctgat gggcgaaatc cgcctgaaca gccagcctat cgggatattg
      301 ctgggtgcac tttgctgcct tgtaagcttg cgcccagcaa caacaccgct atgcccaagt
      361 ctccttcagt aatttccggc aggccgatcg tcagcattga gttggcggtt tcaataagcc
      421 taccacttgc cgcgcagagc cgaccgccgg atccttcact ggtcgttaga cgagagcgtt
      481 catcatatgg ttgacggttg cgacacttgc cgagcgctgc gcggtactcg cgcggcgccg
      541 gctaccgcat tctacaacgc ataaaacgtc tttgggttgt cgtagagaat tttctgcacc
      601 gcggattgcg gaatatcgcc tttgtccgcc cgctcctgca gtattttacg aaactcgagc
      661 tccatggacg gatctcgatg ggtatagtcc gagcccacca ttaagttatc ctcgccgaca
      721 cattggatga tgtaaggaag atcttcatcg acctgacatg tgatgtagac acgattggcc
      781 ttgaacacgt tctccgctag ctcaacctga ggaaccccga gcacaccgag cgccgtgcgg
      841 cccttctgtt tctcctgctg ccgccgtaaa tcgtaggcga cgaacggcgc ccacatcgaa
      901 ccggcctcga tgcagccgat gcgcagcttg gggaacttgg ctggaatatt gtgcaatatc
      961 agacccagca cgccgttgat cgccgctccc ttcgtacgca gaaactgccc atgggaaaat
     1021 tcccgagccg gcgagaagtc cggaacaccc gaacccgtgt gaatgcagat gggcagatcg
     1081 agcttttcgg cttccgcata gaatggttcg aagtacggat cggccgggta cttcccgcct
     1141 tctcgatcac ccttctttaa cacaccgcag gcgccgtgct ctttcgccca gtgcatctcc
     1201 tccagagcgg cttccatgtt ctgcagcggc ggcatacaca cccagcgcaa tctgccgttc
     1261 gattcaccgc atcgatcgcc caaccagcgg ttgtagctgc gccgcatcgc ggtgttaagc
     1321 tccggttttt ctgacgcttc cattaggaaa agcgtcggat agatgacctg gatgtcggta
     1381 cccagttcgt ccatgtgctt gagccgggct ttgacatcta acaattcacg ggtctccacg
     1441 gtggtgccgg aatcgacatc gctgcggatg aaacgaactt ggcgtttgcc gtcaatcatc
     1501 cagtagcgcg ccggcggcaa cttcgggtcg ggattggccg gataggttgt cgagggtttg
     1561 aatgacaact cagcgtcgcc gaagtattcc caggtcgcat cggtttcatc gatatgggtg
     1621 tctgcatcta tgattcgcac tgcatctctc cttacatggt atttcatcga cttctaatat
     1681 gcttttgacg agttttaaag agctattcga ggccagaggc tcgaacagtc tgatacgcac
     1741 ataatgatca agcgcgcaat acgggtcatt tcccttttgt acctactaaa gttacaaaac
     1801 gaccgacgcc gatccagccg gctaatgttc aagggtttcg tgcttaacga caaagccaat
     1861 cggtgaacag gatcgaagtc ctttattcta cacgacatga ctgtcattga ttagaaccgt
     1921 aggatccctt tttcggttga cgccgctcag agcgcgatgt taagccaagc ctgttgatct
     1981 actcctgagt tcttcggctc aatcgatttc gccgctagct cttgaccttt aactttgttc
     2041 ttcgaagagg agaagtaaac tcatgaacga tgttccggtg atcgatgccg acggccatgt
     2101 cttagaaagt gatagcgagc tggcccagta tttcgaaggc gcttatcagg gccacaagcg
     2161 ctccggagtt ttctccatct tcccttctct cgatggatgg ccgcgcggct ttgtcaaagg
     2221 catggacaag gtcagcgcga catcagccga agcctggatc gaatttgccg atgaggcaag
     2281 actccaagcc gcggttctct acccgaccgc gggcctggcg ttcggtttga ttcaagaccc
     2341 ggactgggcc tgtgtggttg cccgggctta taacaactgg ctcactgatc gttactgcaa
     2401 agcagattcg cggctcaagg gtgtcgctat gttgccggtc cacaacccgc aggaagccgc
     2461 cgctgaactg aggcatgcgg tgctcgagca aaacatggtg gcgggaatgc tgccggcggt
     2521 aacgatgctg aataagggat atggtcatgc agacttccat ccgatctatc aggaagccca
     2581 gaaacttaat gtgcccttgg cggttcacgg cgcggtgagc cgcggccttg gttttgattt
     2641 cttgcagacc atgtcgatga ttcacaccct cgagcatccg atcgcgcaga tgattcaact
     2701 gaccagtgta gtcctggatg gtgtcttcga tttgtatccc aagttgagaa tgggtttcct
     2761 cgaggccggc gccggttgga tcccgtacat gatggatcgt atggatgaga agtttcacat
     2821 tgatcggaaa cgcaaacact ttccgctgag caaaccgcca agcgagtatt tcaagagcgg
     2881 caacgtttac gtcacttgcg agaccgacga gaagatcctc gatgcggtca tccgtgagat
     2941 gggagaggat ttcatcatgt acccgacgga tttcccccat gagcgccagg ccggcgaatt
     3001 tgccacagac atcccgaaat ttctaaaacg gccggacctc tcggaaagtg ccaagcgaaa
     3061 aattcttagc gagaatgcaa agcggtttta cggcattgag taaacctatc gagcaaagcg
     3121 atttttaatc gacactgggc caaaccacga gagcaggtac g
//
LOCUS       DASUTG010000753         8159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1596108, whole genome shotgun sequence.
ACCESSION   DASUTG010000753 DASUTG010000000
VERSION     DASUTG010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596108"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(462..773)
                     /locus_tag="VF089_17435"
     CDS             complement(462..773)
                     /locus_tag="VF089_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175798.1"
                     /translation="MNATFTKLSAVAIAFSMSVAVPAAYSQGKGNGKERRKQNVEAKE
                     KHGREAGELPHGLEQFSEKKGNLPSGLQKKKDENESLTRGLEQGGKKRTSNSKGKKSS
                     K"
     gene            complement(1218..1646)
                     /locus_tag="VF089_17440"
     CDS             complement(1218..1646)
                     /locus_tag="VF089_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175799.1"
                     /translation="MKKVPHCFAIAVSLLIVAAAPTPNFAQTSSASSTPATNISNDYS
                     YFLKNVDSQQVVSDLRNGQWTTTTTDPTTNVTTTTTEALPTGKMGFGNVKISLALAQE
                     SLRQQNIMQPTSEQLHTALVGGQMVPGDSTTTTNGILQMR"
     gene            complement(1665..2090)
                     /locus_tag="VF089_17445"
     CDS             complement(1665..2090)
                     /locus_tag="VF089_17445"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX6175800.1"
                     /translation="MQQNSTRAFLVVGLSLVLNGCAAPVALTLFGVGAGVTTGTAVSY
                     TMDGIAYRTFTASLPQVERATLTALNDMGMEIGGTEKTNEGKAIKAKGLDRQIEIELE
                     AISSKTTRIRTVAKDGIFFKDRATATEIILQTERILGKA"
     gene            complement(2715..3170)
                     /locus_tag="VF089_17450"
     CDS             complement(2715..3170)
                     /locus_tag="VF089_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175801.1"
                     /translation="MRFTNVRGLDFALKQLVRSWSQDKLLDSGQVKVLIVEDYPDMRE
                     LLRLILKSLGYIPVLASHGKEGVEKAISEKPKLILMDIRMPIMDGLEAARALRDHPDT
                     KEIPILLMTALLHPPDRAACLEAGCDAYMVRPFNVLDLQNKIRELLTGQ"
     gene            4113..5255
                     /locus_tag="VF089_17455"
     CDS             4113..5255
                     /locus_tag="VF089_17455"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175802.1"
                     /translation="MIKAWDADGHVSESEVTFSDKYWDAKLKDRRPTIIETGPDGPLY
                     WMVDTRSFPVRSGPYQQAGFPLSKNGLAAKVTPGISPHDPVESAEMRSVKARLEQLDR
                     ENTEIQVIYPTMFLSYPITYDRQLAVAMMRAYNSWVADLASQCPERLKWVTIIDPVDP
                     QASVGEIERGKELGAVAVMLLGVTGDVRIDDPPMEPIWAAANDTRLPVAVHTGHSFRA
                     LGEVNDTHHDKTAISFWLTVQFAFHRVISKGIADRYPNLRIAFLEAGCSWVPALAERI
                     EEYSGFPGARAGVDFHRGYTGKHLPKEYIERGQIYFGFEVDEKLLPFAIEEFGDQCWV
                     YGSDIPHGDRLYGAVDVFLKREDIGETSKRKLLVDNTARFYGLTVR"
     gene            complement(5848..6900)
                     /locus_tag="VF089_17460"
     CDS             complement(5848..6900)
                     /locus_tag="VF089_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175803.1"
                     /translation="MNQKNTLSRSIGSATFMSSIALALIICAASAGKLFAAAGDSLDQ
                     LYEKAKKEGKITLYAPLSPHAMSVIPPAFMKRFPGVTVDHIDATSDKLIARVLAEQKG
                     GRVIADVISGALSYLPQILDLKIVEHVVIPEAAAYPAQLKGDLWAATDMQFYIAGWNT
                     NLVKKGEEPKSYEDLANPKWKDSIMGEPRDFQMLVGFAKRKYNNDEKAIDLFKKIAAN
                     QVEFHRGHSQLTEFLVAGQRAICFTCYSHHFPGRIKKGAPIQPLLTEGVGEVGGSVMV
                     MKGAPHPNAALLWVRWAVSEEGQRVYAQAGETPAHPKVEPLEKIRPTTPYMLTIDDVR
                     EFPRYEKTWKEIFQIR"
     gene            complement(7237..>8159)
                     /locus_tag="VF089_17465"
     CDS             complement(7237..>8159)
                     /locus_tag="VF089_17465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175804.1"
                     /translation="FAYLHHISLPCRDVEESKRFYTQVLGGELIHDIPGFAEVRIADI
                     IFGMSQQSGGWTGPTVEYPHYGLNVDGKNFALARRWLEACRVPQHGWTRNYKTALLYF
                     RDPSGNLFELYCDSGYEDIRELALGPRQGGSPLPLEALQYQWNGAIGNAAERPRIHSF
                     GHLSVPVRDIEQSKRFFIEVMGGEPMATSDPDTFTEVRLAGAVVGLSTRGGVPTGRDV
                     EYPHYAFHADAENFLEMIEWLKYHGVVTPGPWTRDGKKGLMYFRDPSGNLLEIYCSGD
                     FPAASTFPRGIKQGGSYNTDYASLYYNWRG"
BASE COUNT         1804 a         2215 c         2175 g         1965 t
ORIGIN      
        1 gtgtgcgatc atccatgctc agagttctcc ataacttggc ctgactcaac ccggatgaca
       61 gcagcggcca cttctccatg aaggtccaaa taatccgcaa tgccgcagta gtgtcggaaa
      121 cgcccccatt cgagctccaa gacggagaca gcctggaggt tagctaacta cctctttgta
      181 tgtcaacggc aaaggctccg ggtggccgca tcgacgcttc cataagagta gggaatgaaa
      241 gagatgcgcc gtagaggaaa aaccggtcag acttgcgcgg ctcgggcagt ccaacaggcg
      301 attcgacctc gaaatgggat agggtgtggc gccaacaagg agtgctctag cagatcattg
      361 atgagaagcg aatttcagca agatcaagat ctcatgcggc tgcggcagcg aataacctgg
      421 gctaacgtgt aagtgcaccg cagcgggacc tagctgcgaa cttattttga actcttcttc
      481 cctttgctgt tcgaggttcg tttcttgccg ccctgctcaa ggcctcgagt tagtgattca
      541 ttctcgtctt tctttttctg caatcctgaa ggaagatttc ccttcttttc ggagaactgt
      601 tcgagaccat gcggaagttc gccggcctca cggccatgtt tctctttagc ctcgacgttc
      661 tgctttctcc tttctttgcc gttacctttt ccttgtgagt aagcggccgg aactgccaca
      721 ctcatgctga acgcaatcgc cacggcagat aactttgtaa aagtagcatt catggtttct
      781 ttcctttcaa ttagacgctt gatcgttatt tcgggccctt gccatgtcct ccgctgttcc
      841 cgtgaccgcc tcccgacgcg ccatgttgat gcgcactgcc cgccccagta acgacgccac
      901 ttgtatggcc caaggatcga ccggacccgc tcacaatgcc gcgcccgccg ttactgatgg
      961 aactagaaga tgcgccggag cttcggcccg aggcactgac aattcccttt ccttgagatt
     1021 cacttccgga aaaggccttg ccgttccctt tacccgtgac tgtagtcgac gatttcccac
     1081 cagcagtagt gatccctttg ctggtcgtag tgctactcgt cgtgctggta gttgtcgtcg
     1141 acgcaggctg cttgccgctc atgagctgac caagtttgac atcatacttt tgcgcaattt
     1201 gtccccaacc catgccttca gcgcatctgc agaatgccat tggtcgtggt cgtggagtcc
     1261 cccggaacca tctgcccgcc cacgagcgcc gtgtgaagct gctcagatgt cggttgcatg
     1321 atgttttgct gcctaaggct ctcctgggct agagccaagg aaatctttac atttccgaat
     1381 cccatcttcc cggttggaag agcctcggtg gtcgtcgtcg tcacattcgt cgtcgggtct
     1441 gtagtagtgg tggtccactg cccgtttcta aggtcgctta cgacctgctg cgagtcaacg
     1501 ttcttaagga agtagctgta gtcattgctg atgttggttg ccggagtgct actcgcagaa
     1561 gatgtctgag caaaattagg tgtgggagcc gccgccacga tcaataacga aacggcaatc
     1621 gcaaaacagt gtgggacttt tttcatagat aacctccttt gtatttacgc ttttccaaga
     1681 attctttcgg tctgtaaaat gatttccgta gcggtcgctc ggtctttgaa aaatatcccg
     1741 tccttggcca ccgtgcggat tcgggtcgtc ttagagctta tggcctctag ttcaatctcg
     1801 atctgacggt caagtccttt cgcttttatc gctttcccct cgtttgtctt ctctgtgccc
     1861 ccgatttcca ttcccatgtc atttagcgcc gttagcgtgg cccgctcgac ctggggaagg
     1921 gacgctgtga aagttcggta ggcgatgccg tccatagtgt atgaaactgc ggtacccgtg
     1981 gtcacgccag cgccgacgcc aaaaagagtc agagccacag gagcagcgca gccatttaag
     2041 accaaactaa gtccaaccac taaaaacgct ctcgttgagt tttgctgcat gctaaccacc
     2101 taaataatat tcgcctgcta cttcaccgcc gtaaaccgag tgccgcttga cttatgccgt
     2161 tgatgctcgt ggtgcagatg ggtcattccc gcgaatcgcc aagggttacg aagccaaggt
     2221 aagcgaccgc gacggttgcc gtttcaccac ttggtcccct gacatacatg gtgaccactt
     2281 ggggtaaaca gttcatagca tcaacgtcga ttgcgatatg tgcgcaacgt ctataccaaa
     2341 atgatggaag gcattttcgt ggagtcgggg gtaataaacg tgtcggttcg gcaaggtgtc
     2401 agagaaaaaa ttactcgatg atggcaaagt cgttcgcgtt acccaccaaa gacgttagct
     2461 ctcgaatcgc gtcggattaa gtaaacagtg ccatttgcgc ggtctttcgc gccctgcttg
     2521 ctgcaagcgc tgctcttcgg agtccaaacg atctgagggc tcagtattaa tgtctcgccg
     2581 tgatctaggt ccggctttct cgtgttgact tatttcctcg tgcaggcagc ctcaaacgat
     2641 acttagaata agaacggtga accgttaccg cctatctaag cgctgtaaga ggagcgacgg
     2701 tccaccagca gctattactg gccggttaag agttctctaa tcttgttctg gagatcgaga
     2761 acgttaaacg gcctgaccat gtaggcatcg caacccgctt ccaaacaggc tgcgcgatca
     2821 ggaggatgga gcagcgcggt cattagtagg attggaattt cttttgtgtc aggatgatcg
     2881 cgaagggctc tcgccgcttc caagccgtcc attatcggca ttctgatatc catcaggatc
     2941 aacttcggct tctctgaaat agctttttcc actccctctt taccgtgact cgcaagaact
     3001 ggaatatagc ctaacgattt taggataaga cgtaatagtt cacgcatatc cggataatct
     3061 tcgacgatca aaacttttac ttgcccgctg tccaataatt tgtcctggct ccatgatcga
     3121 actaattgct tcagcgcaaa atcgaggcca cgcacattcg tgaatctcat tcaacgcctg
     3181 acacgcgatg ggtatctctg acgaccattt tacctgaccg tatgtagagc agtttgtcag
     3241 gaagtcattt cagattctta gtcgtgacac tcgtaagcct gataagctgc gccatcttct
     3301 atgttgaatc cacaaagcaa ctctcgatcg tgaagtactg ctaaaacgga tccgtgaact
     3361 agatgccgag attacccagc atgagcgaat ctgcgggatg gtgaggcatg gctacccagt
     3421 gaaaaacact tgcgactaaa gcttcggacg ttagttgaga cttagtttgg gcacagcagg
     3481 taagacactg atataagggg agcgatagga taatacaaag gggataaagg ctagccgccc
     3541 cggaggggac caggcgatgt ctcgcaggtc cgacactttc gtccacaaga cagtattttc
     3601 aaattttcgg tttactttag ctcactaatc gccgtcgtaa gcctcagcca gtcccgaaac
     3661 gtatacgctc catcgacgtg ttcaactgcg ggatgcgccg ctttgctgcc gagctcgttt
     3721 agacgctctc gacaaaggcc aaactcccaa cggggatcgc tgctgcgaat ggattccagc
     3781 gtgccctctt gaacagcttg agcggagcgc agcgacttct aagcgctcaa acgatgcctt
     3841 ttatgttatc tcaagttatt aaattaagaa gttgccgtgc tccaacccgg aaagatgctc
     3901 taggtcggtg gttcaagcac gcacattccg ctcgcgtttg tacgcatagg cttgctattg
     3961 gaaaagcttc ttggagcggc gagcggaatc aaccacgtgt ggtgagataa tcgcgatcct
     4021 gtcactttgg gaaagcttga gccaaacgag cgcgcgttgt acagctagat agaaaagttc
     4081 tgcaggatca atttggataa agaggcgcac atatgattaa ggcgtgggat gcggacggac
     4141 atgtgtcgga atcggaagtc acgttttccg acaaatactg ggatgctaaa ctgaaggacc
     4201 gaagaccaac catcatcgag accggcccgg atggcccgct ctactggatg gtcgataccc
     4261 gctcctttcc cgtgcgaagc gggccctatc agcaagccgg ctttccattg agcaagaacg
     4321 gcctggcggc aaaggtcact ccgggaatca gtcctcatga tccggtcgaa agcgccgaga
     4381 tgcgctcggt caaagcgcgt cttgaacaat tggatcggga gaataccgaa attcaggtga
     4441 tctatcccac gatgtttctt tcctatccaa tcacctatga tcggcagttg gccgtcgcca
     4501 tgatgcgcgc ctacaacagc tgggtcgccg atctcgccag ccaatgtccc gagcgtctaa
     4561 agtgggtcac gattatcgac ccggtcgatc cgcaggcatc ggttggcgaa atcgagcgcg
     4621 gcaaggagct gggagcggtg gccgtgatgc tgctcggcgt tacgggcgac gtgcgcatcg
     4681 acgacccgcc gatggagccg atctgggccg ctgccaacga caccaggctt ccggtagcgg
     4741 tgcataccgg acacagcttt cgcgcgctgg gcgaggtgaa cgacacgcat cacgacaaga
     4801 cggcaatatc gttttggttg acggtgcagt tcgcctttca ccgcgtgatc tcgaaaggga
     4861 tcgccgatcg ctatccaaat ttgcggattg cgtttcttga agcgggttgc tcctgggtcc
     4921 cagcgctagc ggagcgtatc gaagaataca gcggctttcc cggcgcgcgt gccggagtcg
     4981 attttcaccg cggctatact ggtaagcatc tacccaaaga atacatcgag cgcggccaga
     5041 tttactttgg ctttgaggtt gacgaaaagc tcctgccctt cgccatcgaa gagttcggcg
     5101 atcagtgctg ggtgtacggc tccgacatcc cgcacggcga tcgcctctac ggcgcggtcg
     5161 atgtctttct caagcgagaa gatatcggcg agacgagcaa gcgaaagctg ctcgtcgata
     5221 acacagcgcg tttttacgga ctcactgtta gataatgagc cgcgaggcga aagtgggtga
     5281 gtcagaacgc aatcggttca ctgcaacgta ctgtaagtac gccttagctc gtcgagtttt
     5341 cgcccgccta gcctatggat cttttgagca gccttaaacc agagtttatc agcaacctcc
     5401 taagggcgca taggtgatga ttgcgcggcg gaggatcggc cgtgttccac gtcatcactg
     5461 cttcataatc tgctcgagcg catcgccgcg tcaccagctc atctaacttc ggcttgcctt
     5521 cgggtaccag cggacgaaca gcgggaagtc acgatccggt cgacagtttc cgccgctggc
     5581 gggtaaaatt ccggctgccg atgaagaatc gcttgagagc cgggcggcgt gatgccgatg
     5641 agtaaagcgg tgcctccgcg gctgagacct gggtcacggt ctaggggcaa cttgagttag
     5701 cccccgtttg acggacaccg tacaagagcc tcaaaagttc tggagaggcg ttcaacagga
     5761 aagccgaagc aattttaacg gtagttttca ttgcagtggt tcccttacgt tgccgcggct
     5821 ggtcagctgg aagaccgctc ccgaagttca acggatctga aaaatttcct tccacgtttt
     5881 ctcgtacctc ggaaactctc tgacgtcgtc gatggtcagc atgtaaggcg tcgtaggccg
     5941 gattttttcc agaggttcca ctttgggatg agcaggagtt tcacccgctt gagcataaac
     6001 cctttgtcct tcttcactga cagcccagcg aacccagagt agtgcggcat taggatgcgg
     6061 ggctcccttc attaccatca cagagccgcc gacttcgccc acgccttccg tcagtagcgg
     6121 ttgaatcggg gcgccttttt ttatgcgccc aggaaaatgg tgtgagtagc aagtaaaaca
     6181 aatggcgcgc tgaccggcca cgaggaactc ggttagctgc gaatgccctc gatgaaactc
     6241 cacttgattg gcggcgattt tcttaaacag atcgatcgcc ttttcgtcgt tattatattt
     6301 tcgtttggca aaaccgacta gcatttggaa gtcgcgcggc tcacccatga tgctatcttt
     6361 ccatttaggg tttgcgagat cctcgtagct ttttggctct tcgccttttt tgaccagatt
     6421 ggtgttccat ccggcaatgt agaattgcat atccgtggcg gcccaaaggt cgccttttag
     6481 ctgcgccgga taggccgctg cctcgggtat gacgacgtgc tcgacgatct tcaggtccag
     6541 tatctgtggc aagtacgaca gcgcgccgct tatgacgtcg gcaatgacgc gtcctccctt
     6601 ctgttcggcg agcactcgtg cgatcagctt atcgctcgtg gcgtcgatat gatcgacggt
     6661 tacgccggga aagcgcttca tgaacgcagg cggaataacc gacatcgcgt gaggggatag
     6721 cggcgcataa agggtaatct ttccttcctt tttggctttc tcataaagtt gatcgaggga
     6781 atcccctgca gccgcgaaga gcttaccggc agaagcagcg cagataatga gagccaaggc
     6841 gatcgacgac ataaaggtcg cggatccgat cgacctcgat aaagtgttct tttgattcat
     6901 ttcccttctc cctgatcttc gttccacgat tctggatcaa tatccggaaa aactctcctt
     6961 gttgggtcgc cggctcgaac tgttttctgc aactcctcca tgtaggaaaa caccgatcat
     7021 gtcaaacctt aggccagaaa caaacgttag cgctacttgc ctcagcagat cagccgatta
     7081 ttgataggtt ctgcgcaact tgttgattcg gggatgagtt caacggtcgt gattctgcga
     7141 acggctatcg gctccgagtg ctcgaggagc cgggggcgtg gctgaccaca cccggaaatc
     7201 aggacgttcc agcggatgcc ttcacgtcgc gcgaggctag ccgcgccagt tgtagtacaa
     7261 actggcgtaa tctgtattgt aactaccgcc ttgcttgatg ccgcgcggaa aagttgacgc
     7321 tgccggaaaa tcgccgctac aataaatttc gagcaggttt ccagacggat cgcgaaagta
     7381 catcaagcct ttcttgccgt cgcgcgtcca cggacccggc gttaccaccc cgtggtattt
     7441 gagccactcg atcatctcca ggaaattctc cgcgtccgca tgaaacgcgt aatgcggata
     7501 ttcgacatcg cgccccgtcg gtacgccgcc gcgcgtcgac aagccgacga cggcgccggc
     7561 aagccgtacc tcagtaaacg tgtctggatc gctggttgcc atcggttcgc cgcccatcac
     7621 ctcgatgaag aagcgcttgg actgttcgat gtcgcgcacc ggtacgctga ggtgaccgaa
     7681 ggaatggatg cgcggacgct cggcggcatt tccaatggcg ccgttccact gatactggag
     7741 cgcttcgagc ggcagggggc tgccgccctg gcgtggtccc aatgccagct cgcgaatgtc
     7801 ttcgtagccg ctgtcgcagt aaagctcgaa aagattaccc gaaggatcgc gaaaatagag
     7861 taacgcggtt ttgtagttcc gcgtccagcc gtgttgaggt acccggcatg cttcgagcca
     7921 gcggcgggcc agcgcaaagt tctttccgtc gacattcagc ccgtaatgcg ggtactccac
     7981 cgttggcccc gtccaaccgc cggattgctg agacatgccg aagataatgt cggcgatccg
     8041 tacctcagcg aatccgggaa tgtcatggat gagttcccca cccagtacct gggtataaaa
     8101 tcgctttgac tcttccacat cgcggcacgg tagactgata tggtgcaagt aagcgaagc
//
LOCUS       DASUTG010000754         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_708586, whole genome shotgun sequence.
ACCESSION   DASUTG010000754 DASUTG010000000
VERSION     DASUTG010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708586"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..405
                     /locus_tag="VF089_17470"
     CDS             190..405
                     /locus_tag="VF089_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175805.1"
                     /translation="MAVVTYIVSFISVAAVSFLLVMVIAGPHSGVLEPGIYTQITFLT
                     GLFTVLIVPVLAVYTALRRSGNRTHAT"
     gene            550..1368
                     /locus_tag="VF089_17475"
     CDS             550..1368
                     /locus_tag="VF089_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175806.1"
                     /translation="MSRLIQVTTSWPFVISLCALLLNDLYLKWNHPGVISGKLSDFAG
                     IFLVSLLLFAGTPSQKRGVAIGICAAFGWWKSPMSAPFIRFVQEAGMPWFGRVVDYTD
                     LLALVMLLPASWFVYSINQYQISRGGLRRATAVPAIGITAVAILGTSSIPLVDTYSFQ
                     PQTTESNMQPGVAADVIRAVAEKNRLKCLECDRLAQRGLYQGHGITMRYEVRPPQAIA
                     FSISGEPTGLPIIFFSTDPNKKMDKLRDDLREEFGRRFGNVQFVETLPNRREGK"
     gene            1563..1790
                     /locus_tag="VF089_17480"
     CDS             1563..1790
                     /locus_tag="VF089_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175807.1"
                     /translation="MSELDRVKEQLVYLRFWLGIMVATEIALVGWLISTAIGANATLW
                     FMGAFGAVALAAGIFLVHRQVERRIDQIGKL"
     gene            2024..2860
                     /locus_tag="VF089_17485"
     CDS             2024..2860
                     /locus_tag="VF089_17485"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6175808.1"
                     /translation="MSESIDRISRHYGTEGLPNRILSALKQAGKNLNSLTVEDLAPLD
                     QFHTRGLAATRELITFAEVEPGWRALDVGSGLGGPARVLASEKNCHVTGVDITKEFCE
                     VATLLSKLTRLEHVTEFRHGDATDIPLEDNQFDLVLTMQIQMNIEDKRRFYGEICRVL
                     KPGGRFVFQDIMSGPGGEIHLPVPWATRRESSFLISIDALRETLKKAGFQIETLEDTS
                     EAALAWRKSQPAAAGLAASTLGLHVVMGEQFSLMQSNQVRNLEQRRVTFVRGAANKPK
                     LA"
     gene            2985..3809
                     /locus_tag="VF089_17490"
     CDS             2985..3809
                     /locus_tag="VF089_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6175809.1"
                     /translation="MTNAIADHWGRGDVYNLIVSALNKASKSLDGLTVEDLAPVDHFH
                     ARGFPATVELADRLAVRHGQHILDIGCGLGGPARYMAKRFHCNVSGVDITKPFVDAAN
                     KLTALLRMEQEVRIEHGDGQRLPYPDSHFDGAYTQHVTMNVADRPRFFAEAYRVLKPG
                     GFFALTEHGLGLKGDPHYPLPWSADGAGAYLVFPSETRAILEKTGFEGIVVEDTGAKY
                     VAGYKLAIEKAEKGALPPLGIHILMGETALQKTRNAARNIEEGRTHPIQLICQKPR"
     gene            3905..>4500
                     /locus_tag="VF089_17495"
     CDS             3905..>4500
                     /locus_tag="VF089_17495"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6175810.1"
                     /translation="MARDPFGRTDKLDDGMLEALVLRFEARGKHPEFSRMLSEYLDAM
                     AIAPTAKVLDIGCGTGLVARAIARRPGFAGKVTGVDLSSYLVAAAKRLGAADGVGDHL
                     DFRTGDVRQLEFPDGSFDALVAHTLLSHVDDPVSVLKEAARVVKPEGAIGIFDGDYAS
                     LTFAHANPDKGKAYDEALIGALVTNPRVMRQMPRLLREA"
BASE COUNT          930 a         1275 c         1335 g          960 t
ORIGIN      
        1 accgacgcct tccgctcgct gcgctgtgca tgcggcgcgg caaaacgcga acgttaggtg
       61 gcatacgaat aagcgcccgt agtgatagca accctcctga ccgtcttagg agtcgccatc
      121 ctgctattag ccctgcttac gggctttggg ttcaggctca ggtttaagcc gtcacgctcc
      181 aagttcttta tggcagtcgt tacgtatatc gtgtctttca taagcgtggc cgccgtttcg
      241 tttcttctgg ttatggtcat cgccgggccg cattctgggg tactggaacc aggtatctac
      301 acccagatca cttttctgac tggcctgttc accgtcctta tcgttcccgt cttagccgtt
      361 tacactgctt tgcgccggtc agggaatcgc acccatgcca cctaacagcg cgttggtgag
      421 cgacgcctgc gccgcggcgc tgcgcgcctt cttcagcgcg ccacaacgcg aacgttaggc
      481 agcatagaag gcgaccgggg tgggacacgt ggttcttaac gcgaagtcat cctagtgcgg
      541 agcaccgtgt tgagcaggct gatccaagtc acgacgtcct ggccgttcgt tatcagtcta
      601 tgcgcacttc tactgaacga tctgtatctg aaatggaatc atcccggcgt catcagcggg
      661 aaactttcag atttcgccgg catcttcttg gtctcgctcc ttctttttgc agggacgcca
      721 tcgcaaaagc gtggcgttgc aattggtata tgcgcggcgt ttggctggtg gaaaagtcca
      781 atgtcagccc cctttatccg tttcgtgcaa gaagcgggca tgccctggtt tggccgcgtc
      841 gtcgactata cggacctgct cgcactagta atgctgttac ctgcatcttg gttcgtctat
      901 tcaattaatc aataccagat atcacgtggc ggcttaagac gggcgaccgc cgtaccggcg
      961 atcggaatta ccgctgtcgc aatattgggc acatcatcca tccctttggt ggacacctat
     1021 tcgtttcaac ctcagaccac agaatcgaat atgcagcccg gggtggccgc ggacgtaata
     1081 cgagctgttg cagagaagaa caggctcaag tgtttagagt gtgaccgcct ggcacagcga
     1141 ggcctgtacc agggccatgg catcacgatg agatatgaag tcagaccgcc ccaagcgatc
     1201 gctttctcaa tctcaggcga accaacggga ctaccgatca ttttcttctc gacagatccc
     1261 aataagaaaa tggacaagct gcgggatgac ttgagagaag aattcggcag gcgttttgga
     1321 aacgtgcagt tcgtggagac cctacccaat cgaagggaag gcaaatgacc gagccaaccg
     1381 cgcgcgcagc gggcctcttt aattcttccg gcatttcatc ttggctcaga aagaataatg
     1441 ctgcctaaca gcgcgttgtt gaccgacgcc ttccgctcgc tgcgctgtgc atgcggcgcg
     1501 gcaaaacgcg aacgttagaa ggcgctgtca acgccaagtc gggaggtgac gttgatcgat
     1561 aaatgtccga gctcgatcgc gtcaaagagc agctcgtcta cctcaggttc tggcttggga
     1621 tcatggtcgc cactgagatt gctttggtcg gctggcttat ctctacggcc atcggcgcaa
     1681 atgcaacgct ttggtttatg ggcgcatttg gcgcggtcgc gctagcggcc ggaatttttc
     1741 ttgtgcacag gcaagtcgag cgtcgcattg atcaaatagg taaactgtga gcatggaagt
     1801 tctcgccggc atccttcttc ttgctgttgt ggcgctcttc gtcggcatcg cgctagatgc
     1861 tgcgtatcgc aagtagcgcc ttctaacatg gcggtgttgc cggacgcggc atcaaccgcc
     1921 tcgcttcacc ctcggccgcg cctgccgcgc cgcaaacctc cacgttaggc ggcatttgag
     1981 gcgcccgagt ccgcggctag ccataatcgg gaggtgaacc ttaatgtctg aatcgatcga
     2041 ccgtatatca cggcactacg gcaccgaggg tctcccaaac cgaattctct cggcgctaaa
     2101 gcaggcgggg aagaacttaa actctcttac cgtcgaggac ttggcgccgc tcgatcagtt
     2161 tcacacccgc gggctcgcag cgacacgcga actcatcacg tttgcggagg tagaacccgg
     2221 atggcgagcg cttgatgtgg gaagcggctt gggaggcccc gcgcgagttc ttgcatcaga
     2281 gaagaactgc cacgtaacag gggtagacat cacaaaggag ttttgcgagg tggcgacgct
     2341 gctgtctaaa ttgacgcgcc tggagcatgt caccgagttc cggcacgggg acgcgacaga
     2401 tataccgctg gaagacaatc agttcgactt ggtcttgacc atgcaaatcc agatgaacat
     2461 cgaggacaaa cggcgcttct atggggagat ctgccgtgtg cttaagccgg gaggacggtt
     2521 cgttttccaa gacatcatgt ccggaccagg gggcgaaatt catcttccag tgccttgggc
     2581 gacccgccgc gaatcgagtt tccttatctc aatcgacgcg ttacgcgaaa cgctgaagaa
     2641 ggcaggtttt caaattgaga ccttagagga cacttctgag gcggctttgg cgtggcgaaa
     2701 aagtcagccg gcggcagcag ggcttgcggc gtccacattg ggtcttcatg tcgtaatggg
     2761 ggagcaattt agcctcatgc agtccaatca ggtgcgaaat ctcgagcagc ggcgagtaac
     2821 cttcgtgcgc ggcgcagcca ataaacccaa gctcgcctag catgccgcct aacagcacgc
     2881 cgcactcgga cgcgcgcgtg agcgcggtgc tttgcaaaga cctgtcggcg cgcgccggtg
     2941 ggcgtggacg ttagccgccc gtgcgccggc ctccggagag ctaaatgacg aacgcaattg
     3001 cagaccattg gggtaggggc gacgtttaca acttgatcgt atctgccctg aacaaggcgt
     3061 cgaagtccct ggacggcttg acggttgaag atctagcgcc ggttgaccat ttccacgccc
     3121 gcggtttccc ggcgaccgtg gaacttgcgg atcggcttgc ggtcaggcac ggccagcaca
     3181 ttcttgacat cggttgcggc ctcggcggcc ccgcgcgcta catggcgaag cggtttcact
     3241 gcaatgtaag cggcgtggac ataacgaagc ctttcgtcga cgcagctaac aagctgacgg
     3301 cgctccttcg aatggagcag gaggtgagaa tcgagcatgg cgacggtcag cgcctgccct
     3361 atccagattc ccacttcgac ggcgcctaca cgcagcacgt cactatgaac gtggccgatc
     3421 gcccgagatt cttcgcggaa gcctatcggg tgctgaagcc gggcggattc ttcgcgctca
     3481 cggagcacgg cttgggactg aaaggcgatc cgcactatcc gctgccttgg tcggcggacg
     3541 gtgctggcgc ctacctggtt tttccgtccg agacgcgcgc gattcttgaa aaaaccgggt
     3601 tcgagggcat tgtcgtcgag gacaccgggg cgaagtatgt agcgggatac aagcttgcga
     3661 tcgaaaaagc ggaaaaaggc gccttgccgc cgcttggaat tcacatcctg atgggcgaaa
     3721 ccgcgttgca gaagacccgc aatgccgcgc gaaatataga agaaggacgc acccatccta
     3781 tacaactgat ttgccagaaa ccgcgatgaa gacccttgcc tgttcgcggc taacccgctg
     3841 ctgcagcgga cgcgcagcag cgcgccgctg agcgcaacgt tagccggctg gagaggagat
     3901 gacgatggcg cgcgatccat tcggcagaac cgacaaactc gacgacggta tgctcgaagc
     3961 actcgtattg cgcttcgagg cccgagggaa acacccggag ttctcgcgca tgctgagtga
     4021 gtacctggac gcaatggcca tcgctcctac cgcgaaggtg ctggatattg gctgcgggac
     4081 cggtctggtg gcccgggcga ttgcacgtcg ccctggcttt gccggcaagg tcaccggcgt
     4141 tgatctcagt tcgtacctcg tggcagctgc gaagcggttg ggcgccgcgg atggcgtcgg
     4201 cgaccacctg gattttcgga ctggggatgt gcgtcagctt gaatttcccg atggcagttt
     4261 cgatgcgctt gttgcccaca cgctcttgag tcacgtcgac gacccagtgt ccgtgctcaa
     4321 ggaggcagca cgggtcgtaa agccagaggg cgcgataggc attttcgacg gcgattacgc
     4381 atcgttgacg ttcgcccacg ccaacccaga taagggtaag gcttacgatg aagcactgat
     4441 tggcgctttg gtcaccaatc cgcgggtcat gcgccaaatg ccccgattgc ttcgcgaggc
//
LOCUS       DASUTG010000755         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1300030, whole genome shotgun sequence.
ACCESSION   DASUTG010000755 DASUTG010000000
VERSION     DASUTG010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300030"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF089_17500"
     CDS             complement(<1..303)
                     /locus_tag="VF089_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175811.1"
                     /translation="MRIYIALLLISLACLPNLVQSKNTVRMPRTQTIGEGVVWRISDN
                     KGEHGDSLPPWKEVQITNVFGFKKRPTVGEKVTVVPLDVNIAPIDLRIIETKRGATC"
     gene            complement(408..791)
                     /locus_tag="VF089_17505"
     CDS             complement(408..791)
                     /locus_tag="VF089_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175812.1"
                     /translation="MFKAEYRHIFDRRWPPAIRYRKFRHCLEWYSYLTKQSFHETYRR
                     IGREFGFDEFRKPDEAQLMKAAMLLRRERANFLKKLAPFVERRREKKLQGSRQPRKAE
                     MEALYTPDWLETSSEKRSADSQHRI"
     gene            complement(872..2008)
                     /locus_tag="VF089_17510"
     CDS             complement(872..2008)
                     /locus_tag="VF089_17510"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEX6175813.1"
                     /translation="MKRYRGEFLRVDMSIKKIIPTVLCFVLCALTTFAQTSEPQVNRD
                     PNQAKFVTSDIDNFWRAFDLATKETDREKKIAIFQTHYLDKGSAGLRDFVRLRIKSAK
                     GLVETIEKLPTFYASARPSTLRIADMEKKMRRSFRRFKQIYPDAAFPDVYFVIGVANT
                     GGTASGSGLLIGAELYGLTPKTPRDEFPAWFKTFLPDVQDEKELLRLVTKALDTALKP
                     VEKLPSIVAHESCHFNQHYPKQTTLLEKSIQEGACDLIAELTAGEVINPALKVYGDRH
                     EADLWREFQAEMNGTDLSKWMYNGFKIKDRPGDLGYYMGYKISKAYYQNAKDKRQAIR
                     DVLNIKDFPDFLDRSRYGEKFAESGKKRVLEKAKPNNGMQRTRN"
BASE COUNT          497 a          658 c          602 g          599 t
ORIGIN      
        1 acaagttgcg cctctctttg tttcaatgat tcttaaatca atgggcgcaa tattcacatc
       61 taaaggaacg acggttacct tttcacccac ggtgggtctt tttttgaaac cgaaaacatt
      121 cgtgatctgt acttctttcc agggtgggag agaatcacca tgctcacctt tgttatcaga
      181 tattctccat accactcctt ctccgattgt ttgggtgcgt ggcatgcgga cagtgttttt
      241 gctttgcacc aaatttggga gacaggcaag agagatcagc agcagtgcga tataaattcg
      301 cattcgggtt ggctcaggtc ataagcataa cgccaggcat cgcccgcccg cccaagccgt
      361 tgactgacat gacaggcggc gtgtcgcggg tcgggtgcat gccgttgcta gatgcggtgt
      421 tgcgaatcgg ctgacctctt ctcgctgctt gtttccagcc aatccggagt atagagcgcc
      481 tccatttccg ccttgcgcgg ctgccgactt ccttgcagct tcttctctcg cctccgctcg
      541 acgaatggcg ctaacttttt cagaaagtta gcgcgctctc ttctcaacag catggctgcc
      601 ttcatgagtt gagcctcgtc gggcttgcga aattcatcaa aaccgaactc cctgccgata
      661 cggcgataag tctcgtgaaa gctctgcttc gtcagatagc tgtaccattc gaggcaatgc
      721 cggaatttgc ggtagcggat tgctggaggc cacctccgat caaagatgtg tcgatactcg
      781 gctttaaaca ttcagcagtt tgattccacc tggaaagcat ctaacgcccg gcatcagcgg
      841 cgcgcgcgaa ccattgatga taagaggctt gctaattgcg cgtccgctgc atgccgttgt
      901 taggcttcgc cttttcaaga acgcgctttt tgccggactc tgcaaacttc tcaccgtaac
      961 ggcttctatc gagaaaatca ggaaagtctt tgatgttcaa aacgtcgcgg atcgcctgtc
     1021 gtttgtcttt cgcgttctgg taatacgctt tggaaatctt gtagcccatg taataaccca
     1081 aatcgcccgg acggtctttt atcttgaaac cgttatacat ccatttgctc aaatccgtcc
     1141 cgttcatctc ggcttgaaac tcgcgccata aatcggcctc gtgccggtcg ccgtagacct
     1201 tcagtgccgg gttgatgacc tccccggcag ttaactcggc gatcaggtca caagcgcctt
     1261 cttgaattga tttttccagc agggtcgtct gcttcgggta atgttggttg aaatgacagg
     1321 actcgtgggc gacgatggaa ggcaatttct cgaccggctt caaggccgtg tcgagagcct
     1381 tagtgaccag ccgcagcaat tccttttcgt cctggacatc gggcaaaaaa gtcttgaacc
     1441 aagccggaaa ctcgtcgcgc ggcgtcttcg gcgttaggcc gtacaattcc gcgccgatga
     1501 gcaagccgct tcccgaagcc gtcccgccgg tatttgccac gccgatgaca aaataaacat
     1561 cggggaaagc cgcgtccgga taaatctgct tgaacctgcg gaagctcctc cgcatctttt
     1621 tttccatgtc cgcaatacgc aaagtgctgg gccgagctga ggcgtagaat gtcggcagtt
     1681 tttcgattgt ctcgaccaag cctttcgcgc tttttattct caagcgcaca aagtcccgca
     1741 acccggcgct gcccttatcg agataatggg tctggaagat ggcgatcttc ttctccctgt
     1801 cagtctcctt cgtcgccaaa tcgaacgcgc gccagaaatt atcaatgtca gaggtcacaa
     1861 atttggcttg attcgggtcg cggttgacct gcggttcgct cgtttgggcg aaagtagtta
     1921 aagcgcaaag gacgaaacac aatacagttg ggatgatttt ctttatagac atatccaccc
     1981 tcagaaactc tcctctgtaa cgcttcaagg caagctttcc tgcgccaatg gagaagaagc
     2041 ctaacgccgc gcatcacgcg cccgccaacc ccttacgcgg agcatgagag ccgtcgtgtc
     2101 gcgggtcgcg tgcatgcgct tgttagaccg cccacgggaa gcggcctgaa gctcccgcgc
     2161 catcaggcag cttccttcag gcggttctaa ctactattgt gccgaatgat tcggccttgt
     2221 atgttgaccg cggttcgcct gcggcgcatc cttgcccgtg tgaaccatca ggggcaccgg
     2281 tgaagctgag ttcggcctga ggccgtaaga gcctgttagt tagattagct cccggtgccc
     2341 tcccgaacgc gtcgag
//
LOCUS       DASUTG010000756         4947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_264453, whole genome shotgun sequence.
ACCESSION   DASUTG010000756 DASUTG010000000
VERSION     DASUTG010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264453"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VF089_17515"
     CDS             <1..306
                     /locus_tag="VF089_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX6175814.1"
                     /translation="AMSMIVHRDKAYYRGRDLVQKMRELIPRQMFEVVIQAAIGSRII
                     SREAVKALRKNVTAKCYGGDITRKRKLLERQKEGKKRMKQVGKVEIPQEAFLAALKV"
     gene            318..968
                     /gene="lepB"
                     /locus_tag="VF089_17520"
     CDS             318..968
                     /gene="lepB"
                     /locus_tag="VF089_17520"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6175815.1"
                     /translation="MSAGKVTKAEGQSLGKSKSTFREYAEAIGMALLLALFIRTFIVQ
                     AFKIPSGSMIPTLQIGDHILVNKLAYGIRVPFLEDYVVDFSKPKRGDVIVFIFPEDRS
                     KDFIKRVIAVAGDEVQIRGKKVSINGKEVEDPHAHFEGDDPQTAGLSNRDDYGPRTVP
                     ENHLFVMGDNRDRSYDSRFWGYVNLDEVRGKAFLIYWSWDGADRWVRWERLASLIR"
     gene            1023..1310
                     /locus_tag="VF089_17525"
     CDS             1023..1310
                     /locus_tag="VF089_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175816.1"
                     /translation="MKGKIVLTGLAVLVLLLVIVNILLMAGNQSLQLSVSERQQYIAE
                     TIQLEALNRQVIGVLAEMALKTNDGQLKELLAGVGLNLSPAPAEPSGAKSK"
     gene            1321..1662
                     /locus_tag="VF089_17530"
     CDS             1321..1662
                     /locus_tag="VF089_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175817.1"
                     /translation="MDEERNEIEERELIRPPAERGANLPLTILLIALVLWFGFQTVQL
                     ASERANLGEARGHQEAAMQEAQKLRTQFESLITKTSELANKGHAGAKLVMEELQKRGM
                     AAQSEAISPQK"
     gene            1745..>4947
                     /locus_tag="VF089_17535"
     CDS             1745..>4947
                     /locus_tag="VF089_17535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6175818.1"
                     /translation="MDAFHPLIQRWFLERFESPTPPQQDGWLSIVRGQPTLIAAPTGS
                     GKTLAAFLWSIDRLFKRAISGELRDQTSVVYVSPLKALGNDIAKNLQQPLDEIFHLAY
                     KEGIMLPEIRTAVRSGDTPARERQLMVRRPPHILITTPESLFILLTSPRSREFLKNAE
                     TVIIDEIHAIAADKRGAHLALSLERLNELAERPLQRIGLSATQKPIEEIARLLVGSRA
                     VQEDGSADCVIVDAGHKRDLELYIEVPDEELGPIIRQDIWAAVYDKIVEQTKNHRATL
                     VFVNTRRLVERVAHQLTERLGEGKVGAHHGSLSRKTRLEVEQRLKMGQYPVVVATASL
                     ELGIDIGHVDLVCHIGAPRTLAVLLQRVGRSGHWLGAIPKGIFYPLTRDDLLQCTAAI
                     RSVRQGELDKIAVIEKSLDILAQQMVATVASMTPVVTADVPASKSQPGGGISEEQLWR
                     MVRGAYPYRHLSRTEYEQVLHMLSEGVETRRSRRGAYIHRDRIHGMLRARRGARLTAI
                     TNGGAIPDTADYDVVEFPSETIVGKVNEDFAIESLTGDIFLLGNRSWRIRRVGSGKVW
                     VEDAQGLPPSIPFWLGESPGRTIELSKAVSDLRRDIGDRTAHRTEAQDWLVSEIRVPA
                     AAAEQMVNYVAETQSVLGTVPGQQSIIAERFFDEGGGMQLVIHSPWGARINRAWGLAL
                     RKRFCLSFDRELQAAATDDGIVISLVEQHSFPLSDVFQMLRPAMIEPSLIQAALASPM
                     FTNRWRWNSTRALAVMRHSGGRKVPVALQRMRAEDLLAAVFPEQMVCGDNRTGPVELP
                     DHPLVNETIHDCLHEAMDVEGFKKIIAAIESGAINTLAVDTPAPSPMAHEILNANPYA
                     FLDDAPLEERRARAVSLRRIDPRLEREFGQLDQSAIDEVCRQAWPEIRNGDELHDFLL
                     GVGILPVEERPDWRPIAEQLVNNRRATEATWLPPGSQTPLRAYVAAERSELVRTCLPD
                     VKFAPPVELPLGFEAQAVSQEEASRRIVQGWMEAVGPTTVTGLSNRLGIESRKVESAL
                     LSLEGTGMVLRGRFTPSNGSDGEVEWCDRVLLARIHRL"
BASE COUNT         1129 a         1317 c         1470 g         1031 t
ORIGIN      
        1 gccatgtcga tgatcgtgca tcgggacaaa gcgtactacc gcggccgcga ccttgtgcaa
       61 aagatgcgcg agctcattcc acgacagatg ttcgaagtcg tgatccaagc cgccatcggc
      121 agccgaatca tatctcgtga ggcggttaaa gcattgcgga aaaatgtcac ggccaaatgc
      181 tacggcggcg atatcacccg aaaaagaaaa ttgctggagc ggcaaaaaga aggaaaaaag
      241 cgcatgaaac aggtgggcaa agtagagatt ccccaggaag cgttcctcgc tgctctgaag
      301 gtttagatcg gagttctatg tcagccggaa aagtcaccaa ggcggagggt caatcattgg
      361 ggaagagtaa atcgaccttt cgcgaatacg ccgaagccat cgggatggcg ctactcttgg
      421 cgcttttcat tcggacgttc attgttcaag ccttcaaaat accatcgggc tccatgatcc
      481 cgacattgca gatcggcgat cacattctgg ttaacaagct cgcctacggc attcgtgtgc
      541 cgtttctaga agattacgtg gttgacttct caaaaccaaa acggggcgac gtgatcgtct
      601 ttatctttcc tgaagaccgc agcaaggatt tcatcaaacg cgtgatcgct gtggcggggg
      661 atgaggttca gattcgtggt aagaaggttt cgatcaacgg aaaagaagtg gaggatcccc
      721 atgcgcactt cgaaggtgac gatccacaaa ccgcgggcct ttcgaaccgc gatgattacg
      781 gtccaaggac cgtgccggaa aatcaccttt tcgtgatggg tgataaccgg gatcggagtt
      841 acgatagtcg cttctggggt tacgtcaact tggacgaggt gcgtggcaaa gcatttctga
      901 tctactggtc ctgggatggc gccgatcgtt gggtgcgctg ggagcgtctt gctagcctca
      961 tccgttaaca gccagagcga agaaatcagc aagatgaacg accgcactat gcgaattcag
     1021 tcatgaaagg gaaaatcgtc ctcacaggat tagcggtgct ggtgttgtta ctggtgatcg
     1081 tcaatatctt attgatggct ggtaatcagt cactgcagct cagcgtcagc gaacggcaac
     1141 aatacatcgc ggaaaccatt caactggaag cgttgaaccg gcaagttata ggtgtattgg
     1201 ctgagatggc gctgaaaacc aacgatggac agctgaagga actcctggcc ggagtgggtt
     1261 tgaacctttc gccggctcct gcggaacctt cgggcgctaa gtcgaagtag gtgtttggtc
     1321 atggatgaag agcggaacga aatcgaagaa cgtgagctga tcaggccgcc ggccgaacga
     1381 ggcgctaatc tgccgctgac gatattgctg atcgcgctgg tgctctggtt tggtttccaa
     1441 accgtgcagc tcgccagcga gcgggcaaat ctcggcgagg cgcgcggtca ccaggaagcg
     1501 gccatgcagg aggcgcaaaa acttcgcaca cagtttgagt cattaattac caagaccagt
     1561 gaattggcta acaagggcca tgccggcgcc aagctcgtga tggaagagct gcagaaacgt
     1621 ggaatggcgg ctcagtccga agcaattagt ccgcagaaat aatcagccaa gtaccatcct
     1681 gttgatcctg ccgcagttta tccttaagct tggttcttct gcctcgacag ttacaggaga
     1741 acgtttggac gcatttcatc cgctgattca gcgttggttc cttgagcgat tcgaaagtcc
     1801 gacaccaccc caacaggatg gttggttgtc catcgtccgc ggccagccga cgctcatcgc
     1861 ggcgcccacc ggatcaggta aaacgttagc cgctttccta tggtccatcg accgactttt
     1921 caaacgggcg atctcggggg agttacgaga tcagaccagc gtggtttacg tctcaccgct
     1981 gaaagccctc ggcaacgaca tcgctaaaaa tttgcaacag ccgctcgatg aaatctttca
     2041 tcttgcttac aaggaaggaa tcatgttgcc ggagattcgc acagcggtgc gctcgggtga
     2101 cacgccggcc cgtgaacgcc aattgatggt gcggcggccg ccgcacattc tcatcaccac
     2161 gccggaatcg ctcttcatcc tcctgacgtc accgcgtagc cgcgagtttc tcaagaatgc
     2221 cgagaccgtg attatcgatg aaatccatgc catcgccgcg gataaacgtg gggcgcatct
     2281 ggccctttcg ctcgaacgac tcaacgagtt ggccgagcgg ccgctgcaac ggatcggctt
     2341 gagcgcgacg caaaaaccca ttgaagagat agcacggctg ctggtcgggt cgcgggcagt
     2401 gcaggaggac ggcagcgccg actgcgtcat tgtcgacgca ggccacaaac gcgatctaga
     2461 gctttacatc gaggttcccg acgaggagct cggaccgatc atccgccagg atatctgggc
     2521 cgccgtctac gataaaatcg tcgaacaaac caaaaaccac cgggccacat tggtgttcgt
     2581 aaacacacga cgtctggtgg agcgcgtggc gcaccaactg accgagcgct tgggcgaggg
     2641 caaggtgggc gctcaccacg gcagcttgtc acggaaaact cgtttagagg tcgaacagcg
     2701 attaaaaatg ggccaatacc cagtcgtggt cgccaccgca tctttggaac tgggtatcga
     2761 catcggccat gtcgacctcg tttgtcatat cggcgcgcca cgcacattgg cggtgttgct
     2821 ccagcgggtc ggccgctccg gccattggct gggagcaatt cccaagggta ttttttatcc
     2881 tttaacccgt gatgatctgc tgcagtgcac cgcggcgatt cgcagcgtac gccaaggcga
     2941 gttggacaaa attgcggtga tcgaaaaatc cctcgacatc ctggcccagc aaatggtagc
     3001 cacggttgcg agcatgacgc cggtagtcac agcggacgtt cccgcgagca aatctcaacc
     3061 cggcggcggc atcagcgagg aacagctgtg gcgcatggtt cgcggcgctt atccgtatcg
     3121 ccatctgtcg cggaccgaat acgaacaggt gctgcatatg ctcagcgagg gagtcgaaac
     3181 ccggaggagc cggcgcggcg cctacattca ccgcgaccgg atccatggca tgttgcgcgc
     3241 ccggcgcggc gcccgcttaa cggcgatcac caacggcgga gcgattcccg ataccgccga
     3301 ttacgatgtg gttgagtttc ccagtgaaac gatcgtcggc aaagtcaatg aagattttgc
     3361 catcgagagt ctcaccggag atattttttt attaggcaat aggtcctggc gtatccgtcg
     3421 ggtcggctca ggcaaggttt gggttgaaga tgcacagggg ctgcccccga gtatcccgtt
     3481 ctggctcggc gagtcgcctg ggcgcaccat agaattatcc aaagcagtat cggatttgcg
     3541 ccgcgacatt ggcgaccgta ctgcacatcg aacagaagcc caagactggc tggtcagtga
     3601 gattagggtt cctgcagcgg ccgcagaaca aatggttaat tatgtagcgg aaacccagag
     3661 cgtgcttgga acagttccgg ggcagcaatc gattattgct gagcggtttt ttgacgaagg
     3721 cggcggcatg caactggtga ttcactcccc ttggggcgcg cgtatcaacc gcgcctgggg
     3781 cttggcgctg agaaagcgct tctgcctgag tttcgatcga gagttgcaag cggccgccac
     3841 cgatgacggc atcgtcatct ctttggtcga gcaacactcg tttcccttga gcgacgtatt
     3901 tcagatgctg cggccggcga tgattgagcc aagtctgatc caggcggctt tagcatcgcc
     3961 gatgtttacc aaccgatggc gctggaattc cacgcgcgcc ctggctgtga tgcgccacag
     4021 cggcggcaga aaggtgccgg tagcgctgca gcggatgcgc gccgaagatt tgctggcggc
     4081 ggtttttccc gagcagatgg tgtgcggaga taaccgcacc ggtccggtcg agttgccgga
     4141 tcatccgctg gtcaatgaaa ctatccacga ttgcctgcac gaggccatgg atgtcgaggg
     4201 attcaagaag atcatcgcgg ccattgagag tggagcgatc aatacattgg ctgtcgatac
     4261 gcctgcgccg tcgccgatgg cccatgaaat tctgaacgct aatccgtacg cattcttaga
     4321 cgatgcaccg ttggaagaac ggcgtgcgcg agcggtttct ctaagacgga ttgatccacg
     4381 cttggagcgc gaattcggtc agctggatca atcggccatc gacgaggtct gccgtcaggc
     4441 atggccggaa atccgcaatg gcgacgaact ccatgacttt ttgctcgggg taggaattct
     4501 acctgttgag gagcgccccg actggcggcc catcgctgaa cagttggtaa acaaccgacg
     4561 cgccacagaa gcgacttggc tgcctcctgg ctctcaaacg cctttgcgcg cctatgtcgc
     4621 ggcagaaagg tctgagttgg ttcgcacctg cctgccggat gtaaagttcg ctccgcctgt
     4681 agagttaccg ttagggttcg aggcgcaagc cgtgtcacag gaagaagcaa gcagaagaat
     4741 agtccagggc tggatggaag cggtcggacc gaccactgta accggtttat cgaaccggct
     4801 ggggatcgaa tcgcggaaag tagagtcggc gttgttatcc ctggaaggaa cgggcatggt
     4861 tttgcgcggc cggtttactc catcgaacgg ttcggacggt gaagtcgaat ggtgcgatcg
     4921 cgtcctgctg gcgcgcattc atcgcct
//
LOCUS       DASUTG010000757         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2042014, whole genome shotgun sequence.
ACCESSION   DASUTG010000757 DASUTG010000000
VERSION     DASUTG010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042014"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VF089_17540"
     CDS             complement(<1..519)
                     /locus_tag="VF089_17540"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEX6175819.1"
                     /translation="MDTWIFQPGYPVITVAKRSGGIALSQRLFRYLDDDAGSTQKWLV
                     PIFLRAGTKRGVVTKTVLLADQELLLELPDEPDWVVVNAGGDGFYRARYSDELMSGLK
                     RALQSILSSVERFGLINDTWAATVAGLTPLSEYLNLVDLLRDESDINVWTTVIGSAHH
                     LNRILDEARRTDL"
     gene            complement(603..1853)
                     /locus_tag="VF089_17545"
     CDS             complement(603..1853)
                     /locus_tag="VF089_17545"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6175820.1"
                     /translation="MSDQKPHRLPPTVTPERYEIRLTPDLSAARFDGEEKIVLQVHEP
                     VRKIRLNAAELVFEAVSMQQPGGAVLPGDVALDRDNEQAELDFHQTLAPGPWELRISF
                     SGVLNDKLHGFYRSTYKDNDGQEKPLASTQFESTDARRAFPCWDEPAFKAVFQPTLVV
                     DQGLTAISNTQIMRETPVPGTSKKEVVFAESIRMSTYLVAFIVGEFESTDPVAVGDAP
                     LRVWAVPGKKHLAKYAEQIGSFSLTYFSEYYGIPYPGDKLDLIAIPDFASGAMENLGA
                     ITFRETALLVDAEKATRGELERVADVVSHENAHMWFGDLVTMKWWNGLWLNEAFATFM
                     EMLAVDAWKPEWRRWDSFTVSRAAAMQIDGLKSTRPIEFKVEKPEEAAGMFDVLTYEK
                     GASVLRMLEQYLGAEPFRDGIRLS"
     gene            2034..2774
                     /locus_tag="VF089_17550"
     CDS             2034..2774
                     /locus_tag="VF089_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX6175821.1"
                     /translation="MSPHDESALLISRRQALILLGTAGATMVLQRPTVYSASAKSKDG
                     TLPLCIVTPKQTEGPFFVDERLKRSDIRVDPSDGSITPGVPLRLTFRVSAIGGAGCKP
                     LSGAIVDVWQCDAIGVYSGVNDSGSQSSEGKNFLRGYQVTETNGSTQFTTIYPGWYPG
                     RTVHIHFKVRVSAKSGRGQELTSQLYFDDGLTDRVHAQSPYAGRGPRTVRNQRDGLFR
                     DGGRQLMLSPVQSGDGYLALFDIGLQMA"
BASE COUNT          638 a          821 c          774 g          613 t
ORIGIN      
        1 caagtcggtg cgccgcgcct cgtcaagaat tcggttcaga tgatgagccg agccgatcac
       61 ggtggtccag acattgatgt cggactcatc acgaagcaga tcgactaaat tgagatactc
      121 ggaaagtggc gttaaacccg ccactgtcgc tgcccaggta tcgttgatca acccgaaacg
      181 ctcgaccgac gacagaatgc tttgcagcgc tcgtttcagg ccgctcatca attcgtcgct
      241 gtagcgcgca cggtaaaagc catctccgcc ggcattcacc acgacccaat cgggttcatc
      301 gggcagctcc agaagaagtt cctgatcggc gagaagaact gttttggtga caacaccgcg
      361 tttagtgccg gcgcgcagaa aaatcggcac cagccatttc tgcgtcgatc ctgcatcatc
      421 gtcgagatag cggaagagcc gctgcgacag agcgatgccc ccggagcgtt tggctacagt
      481 aatcaccgga taacccggtt gaaaaatcca ggtgtccatc aacgcgcgca ccggctgacg
      541 ggtggaatct tcgatggcgt cccatagatc cgttgtctcg gcattggcat actgatgccg
      601 ccttaagaga gccgaatgcc gtcgcgaaac ggctcggcgc cgagatactg ttcgagcatg
      661 cgcaaaaccg acgcgccctt ctcgtaggtg agcacatcga acatgccggc agcttcctcc
      721 ggtttttcca ccttgaattc gatgggacgc gtgctcttga gcccatcgat ctgcatcgcg
      781 gcggcgcgcg acaccgtaaa actatcccag cgacgccatt cgggtttcca ggcatccacc
      841 gccagcattt ccatgaaggt ggcgaatgcc tcattaagcc aaaggccatt ccaccatttc
      901 atcgtcacca gatcgccaaa ccacatgtga gcattctcgt gcgataccac gtcggccact
      961 cgctcgagct cgccgcgggt tgccttttcc gcatccacca gcaatgcggt ttcacgaaac
     1021 gtgatggcgc cgagattttc catcgctccc gatgcaaaat ccggaatcgc gatcagatcc
     1081 agtttatcgc cggggtaagg gataccgtaa tattccgaga agtaggttaa agaaaagctg
     1141 ccgatctgtt cggcgtattt tgcaagatgc ttcttacccg gcacggccca aacccgaagc
     1201 ggcgcatcgc ctacggccac cggatcggtg ctttcaaatt cgccgacgat gaacgcgacc
     1261 aggtacgttg acatgcggat actctcggcg aagacaactt ctttcttaga agttccaggc
     1321 acaggcgttt cacgcataat ttgcgtattg gaaatggcag ttaatccttg atcgacgacc
     1381 agtgtcggtt gaaataccgc cttgaacgcc ggctcatccc aacagggaaa cgcgcggcgg
     1441 gcgtcggtag attcgaattg cgtcgaagcc agcggctttt cctgaccgtc attgtctttg
     1501 taggtgctgc ggtaaaagcc atgaagtttg tcgtttaaca caccggaaaa actgatgcgc
     1561 aactcccaag gtcctggggc caaagtctgg tggaaatcca attcggcctg ctcgttgtcg
     1621 cggtccagag ccacatcccc cggcagcacc gcaccgcccg gttgctgcat tgaaaccgct
     1681 tcaaacacca gctcggcagc gttgagccga atctttcgaa ccggctcgtg cacctgaagc
     1741 acgattttct cttcgccgtc aaaacgcgcg gcagagagat cgggagtcaa tcggatttca
     1801 taacgttccg gggtaacggt cggcggcaag cgatgtggtt tttgatctga catcgaaact
     1861 cctatggatt ggttcagacg agttgcggat ccgggtttat ttttacacta ccacgatgcc
     1921 gtaactcaat cgtgctgctg caggcgcagc aggaaccagc tcggcgactt ttattcggcg
     1981 acttttataa gggagtattt gcggcagtca tgggcacttg ctagcatccg gccatgagcc
     2041 cacacgatga atcagctttg ctaatcagcc ggcgccaggc gctgattctg ctcggcaccg
     2101 ccggggcaac gatggttctg cagcgcccca ctgtttattc cgcctctgct aagtcaaagg
     2161 acggcacact gcctctctgc atcgttacac caaagcagac tgaaggaccg ttctttgttg
     2221 acgagcgcct gaagcgatcg gatatccgtg tcgacccgtc ggatggtagc atcacaccag
     2281 gcgtgcctct gcgcttgacg ttccgcgttt ctgcaatcgg cggcgcgggt tgcaagcccc
     2341 tatccggcgc catcgtcgat gtctggcagt gtgacgcgat cggagtttat tccggcgtca
     2401 acgattctgg atcgcagagc agcgaaggga aaaatttttt gcgcgggtac caagtcacgg
     2461 agaccaacgg cagcacacag ttcacgacaa tttatccggg atggtatccg ggcagaacag
     2521 tacatatcca tttcaaagtc cgtgtcagcg ccaagtcggg aagaggtcag gaactcactt
     2581 cgcagcttta tttcgatgat gggttgacgg atcgcgtcca cgcgcaatcc ccatatgccg
     2641 gccgagggcc acgtacggtt agaaaccaac gggatggcct gttccgcgac ggcggccggc
     2701 aactgatgct gtcaccggtt caaagcggcg acgggtacct agccctattc gacatcggcc
     2761 ttcagatggc gtaacaggtg cgaaaaaaca cgcgagtgat tcgtagcgct caagcctctg
     2821 gaacttttag cagcctgcac cgagtt
//
LOCUS       DASUTG010000758         4753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1006418, whole genome shotgun sequence.
ACCESSION   DASUTG010000758 DASUTG010000000
VERSION     DASUTG010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006418"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..271
                     /locus_tag="VF089_17555"
     CDS             56..271
                     /locus_tag="VF089_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015220173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6175822.1"
                     /translation="MKLTAVIEREGEGYVSLCPELDVASQGKTVEEARDNLREALELF
                     FESASPDEIKQRLRSEVFVTQIEVAVG"
     gene            264..483
                     /locus_tag="VF089_17560"
                     /pseudo
     CDS             264..483
                     /locus_tag="VF089_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            740..961
                     /locus_tag="VF089_17565"
     CDS             740..961
                     /locus_tag="VF089_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX6175823.1"
                     /translation="MQYTVVVHSAEGGGYWVDVPSLPGCFSQGETIDEAMNNAREAIE
                     LHIQGLKDDGQEVLTEKDLVIGRVDVKAA"
     gene            970..1269
                     /locus_tag="VF089_17570"
     CDS             970..1269
                     /locus_tag="VF089_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175824.1"
                     /translation="MSIYDTLWWLKFPRYGDEYIGCEWIRVSAQGVPAHIGTPTPGFG
                     YKDGDPYAEFLPPVVTVNAEGESEFMRAVVVVTEESRHSALPSRVRQSNTCSFWP"
     gene            complement(1858..2304)
                     /locus_tag="VF089_17575"
     CDS             complement(1858..2304)
                     /locus_tag="VF089_17575"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6175825.1"
                     /translation="MNALELLKTDHKTVKELFEKAEGSENKKQQLQLFEKIKTELETH
                     THIEETVFYPAVAKNEELKDMVLESLEEHKQVKTLLREMENLAADSEKFEPKLKVLME
                     NVEHHAEDEEEGKMFPKIRKLMNAEALDQLGRELEAAKNKEVRKAS"
     gene            complement(2404..2943)
                     /locus_tag="VF089_17580"
     CDS             complement(2404..2943)
                     /locus_tag="VF089_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX6175826.1"
                     /translation="MSKSVFCLSDNESQAERMVQELKAAGFSNNDISVLFPDKSGTKD
                     FAHEQHTKAPEGAATGAGTGGVLGGALGWLVGIGSLAIPGVGPFIAAGPIMAALAGAG
                     IGAAVGGLTGALVGMGIPEYEAKRYEGKIKEGNILISVHSEDSDEVKRAKDIFERGGA
                     HDISYSEEKGVPKQEERRV"
     gene            complement(2961..3356)
                     /locus_tag="VF089_17585"
     CDS             complement(2961..3356)
                     /locus_tag="VF089_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6175827.1"
                     /translation="MRTSALGFALAFLLVGCSKNDVSRNDQPSQTATVAADNSGLNVR
                     DRDDATKTSGDQSESEADRTITQNVRQAVVADDSVSTNGKNVKIITVDGTVTLRGPVK
                     SEKEKTNIGAKAQQIAGVKRVDNQLEIAN"
     gene            4098..4307
                     /locus_tag="VF089_17590"
     CDS             4098..4307
                     /locus_tag="VF089_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175828.1"
                     /translation="MDTQDIDGVLKSPGQRLDVRPGRVVLQSKTQEGGTEYLLRVFVD
                     IDRVPAEIVTAYRTSKVAKYWRDQQ"
     gene            4304..4504
                     /locus_tag="VF089_17595"
     CDS             4304..4504
                     /locus_tag="VF089_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX6175829.1"
                     /translation="MKVLYDPHTDTLNVILKDNVAVHESDEDKPGVILDYDKEGNLIS
                     LEILDASKRVTDTRKVDFQLAE"
BASE COUNT         1091 a         1224 c         1227 g         1211 t
ORIGIN      
        1 tcaagttggc gaggttgcgg agccttgata tactcgtgtc gtaacggagg tgccaatgaa
       61 actcacggcg gtaattgagc gggagggaga gggatacgtg tcgctgtgcc ctgagctgga
      121 cgtggctagt cagggaaaga ccgtcgagga agcccgtgat aatctgcgcg aagcgctgga
      181 gcttttcttc gagtcagctt cacctgatga aatcaaacag cggctgcgca gcgaggtgtt
      241 cgtaacccaa attgaggtag cggttggcta gattgcgcat tctttccggg aaggaagtct
      301 gtgccatcct aagaaaatcc ggctttgagg aagtccgcag gcgaggcagt cacgtggtca
      361 tgaaaaaaaa taacgtcagg aaggattact gttccagtac ctgatcacgg tgaaattcgc
      421 attggcaacc tgcaatcgat catccgtcag tccggcgtaa cccggagcga gtttgaggag
      481 tgatcaccca atcaattttc aattttggat tccgcctgga tcagcgctga atataaattc
      541 cgtcggcaag cccagaactc cgggatcgga cccagctaag ttgcagtaag gcacaatatt
      601 cgttccaaaa tctagtattc tccgacctta gcaccttatt ttgaccgcta aagctcaaaa
      661 taggcttgct gaaagccgtt atccgcaggg cgggattgac cgaggaacag tttttggact
      721 tgctttagag gagtacgtaa tgcagtacac agttgtcgtg cacagtgcag agggaggcgg
      781 atattgggtg gatgtgccgt ctcttcctgg gtgtttcagc cagggtgaaa caatcgacga
      841 ggccatgaac aatgctcggg aagcgatcga gcttcacatc caggggttaa aagacgacgg
      901 gcaggaagtg ctgacggaaa aagacctggt gatcggtcga gtggacgtca aagccgctta
      961 gagtgtccaa tgagcattta cgataccctt tggtggctca agtttccacg ctatggcgac
     1021 gagtacatcg ggtgcgaatg gatcagagtt agcgcccaag gggttcccgc gcacattggt
     1081 acacccacgc cgggattcgg ttacaaggac ggcgacccat acgccgaatt cttgcctccc
     1141 gtcgtgacgg ttaacgccga aggtgaaagt gaattcatgc gtgccgttgt cgttgttact
     1201 gaagagtcaa ggcacagcgc gctcccctca agagtacgtc aatccaatac ttgttctttc
     1261 tggccgtgag tacgcgtcga tctcgtttgt cgagttgcac acgcgcatct gcgatgcgct
     1321 acgcgggtca ggtccggcgg tcgtcgcaca gtcgtttaca gccgacggtg aggtgcagtt
     1381 ggtaatgtcc gaccgacgaa ttctcgatac aaggaagagc gggagaggag ttgtggaaaa
     1441 ggattagaca cctacggatc cctggtacaa agttgcaacg ccgaggaagg tagtacgcga
     1501 aggacgctct ttcaaccgga tgagttcgcc attgctctgg aacaggtcgt tggggggaac
     1561 gcgccggaag attatcgtga cccggagaag tttttttcgc gcacctgctt cacgcgcgcg
     1621 ctcaaagatc acgcggcatg gttctccagc gactgtcggg caagaccgaa tgcgccgccc
     1681 cggtcctgaa tttatccaca tgaaaccagt atcgaacgtt cgcaactgat gatgcgatcc
     1741 gcccggcggt tctgccgttt atagcctggc agccgccgag cgaaagaaac agcaccagca
     1801 cgaatctgga agtctgatat tttaacttcg agtccaattt agacaggaga gccgtcgtta
     1861 gctggctttt cttacttcct tattttttgc cgcttccagc tcgcgaccta actgatcaag
     1921 agcctcagca ttcatgagct ttcgtatctt gggaaacatt tttccctctt cctcgtcttc
     1981 cgcatgatgc tctacgtttt ccattaacac ttttagttta ggttcaaact tctcgctatc
     2041 tgcggccaga ttttccatct cgcggaggag agtctttacc tgcttgtgct cctcgagaga
     2101 ttcaagcacc atatctttta actcttcgtt ttttgctacg gccggataga aaaccgtctc
     2161 ctcaatatgc gtatgggttt ccagttccgt tttaatcttt tcaaaaagtt gcaactgctg
     2221 tttcttgttt tcgcttccct ctgccttttc gaatagttct ttcaccgtct tgtggtctgt
     2281 cttaagaagt tctaaagcat tcatttactt tcctcaaaaa aaaggtcgat gtaatattcg
     2341 atacgaacaa gtcttaaagg tgcgagtccg cccatctagc tgcggactcg caacaaagcc
     2401 cgcctaaacg cgcctttctt cctgcttggg aacgcctttc tcttcgctgt acgagatgtc
     2461 gtgggcgccg ccgcgttcaa aaatatcctt tgcgcgcttt acttcatcgc tatcttccga
     2521 gtgcactgaa atcaggatgt tgccctcttt gatttttccc tcgtatcgct tggcctcata
     2581 ctcaggaata cccatgccta cgagcgctcc ggttagcccg ccgacggcgg cgcctatacc
     2641 cgcaccggcc agagcggcca tgatcggtcc ggcagctata aatgggccga cgccaggaat
     2701 ggccaagctc ccaatgccca ccagccatcc caaagctcct cctagaactc ccccggtgcc
     2761 tgctcctgta gcggccccct ccggtgcctt ggtgtgctgt tcgtgagcaa aatccttcgt
     2821 tcccgatttg tcagggaaca ggaccgagat atcattgttg gaaaagcccg cagccttcag
     2881 ctcttggacc atcctttcgg cttggctttc attatcactc aaacaaaaaa ctgattttga
     2941 catacttttc tcctttaaat tcagtttgcg atctccaatt ggttatcgac tctttttaca
     3001 ccggcaatct gctgcgcttt ggcgccgata ttagttttct ctttctcact cttaaccggc
     3061 ccgcgtagcg ttaccgtgcc atcaaccgtg atgatcttta cgttcttgcc attcgtggag
     3121 actgagtcgt ccgctacaac agcctgccgg acgttttgag taattgtccg gtccgcctca
     3181 ctctcagatt gatctcccga agtctttgtc gcatcgtcac gatctcggac attaaggcca
     3241 ctattatccg ccgctaccgt ggctgtttga ctaggctggt cattccttga cacatcattt
     3301 ttgctacacc cgactaatag aaacgctaga gcaaaaccta aagcacttgt tctcataaat
     3361 cctcctttcg ttaagatctg ttttctccgg agttgctgat ttccgaaact cgtccttatt
     3421 aaaatccact gcgcttaaca ccctcgattt gggagtctgc ggactccttg ctgaccaact
     3481 tctcgcatac ctcgtaggag aattagtcgg cgcaatcgga acgcttgcgc gaatcggcag
     3541 tagcgtcttt ctctaggcgt cagcgagatt cgtcaatcgc tgatttcagg agggttgtga
     3601 ttcgaggagg tagcgtaagc cttacgagat ggagtgcata cctgctcctt ctctgtaatc
     3661 agttaatcgg ctttgtcaat ggcgagattc gctctttctt cttcgcccgg gcagatttcc
     3721 gtacaaaatc gggcggctag gcgatcatct cgtgttccgt tagtaaatat cccctgctac
     3781 agcgccacga tgccgaaagt agccggttcg cttttcgtcg gaactatgcg agtccggccg
     3841 agggcatgct gcaatgctct ttcaagcgag cgcacgaatg ggttgagagc tgtgctcaag
     3901 tctgagcagg accgcgggcc ggatttcctt cgtttggcga atgcgctgtg ggcattgtat
     3961 cctaaggaat gtgaagagaa gcgctgctcg atgccatgtt gctggcggtg ccgaggtaag
     4021 gaaaacccgt ggccgctgac ccaatcgtcg attatgtcct cacagaccac gcggttgggg
     4081 agctgaggag gcggcagttg gatacacaag atatagacgg ggtgctaaaa agtcctgggc
     4141 agcgtttaga tgtgcgaccg ggtcgtgtcg tcttacagtc caaaactcaa gaaggtggaa
     4201 ctgaatactt gctaagagtc tttgtcgata tcgaccgggt gcctgccgag atagtgacgg
     4261 cttaccggac aagtaaagtt gcaaaatatt ggagggatca gcaatgaaag ttttatacga
     4321 tccgcatacg gacacgctga acgtgattct aaaagacaac gtcgccgtac acgaaagtga
     4381 cgaagataaa cccggcgtca ttctcgacta cgataaggaa gggaatctta tctcacttga
     4441 aattctcgac gcttcgaaga gagtcacgga tacccgcaag gtcgattttc aactggctga
     4501 atgaccgatg gatccctggt ataaagttgc aacgccgagg aaggaagtac gcgaaggatg
     4561 ctcgtttaac cctgacgagt tcgccatcgc tctggaacag aacgttgggg ggaacgcgcc
     4621 cggaagactc gtgacccgga aaagtttttc tcgcgcacct gggctaaagc acaacctcca
     4681 ttatcggacc aagctaagtt gcggtaagtt cagaatcttc gttccaaaat cttatttccg
     4741 agcttagcgc ctc
//
LOCUS       DASUTG010000759         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2192364, whole genome shotgun sequence.
ACCESSION   DASUTG010000759 DASUTG010000000
VERSION     DASUTG010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192364"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VF089_17600"
     CDS             <1..329
                     /locus_tag="VF089_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEX6175830.1"
                     /translation="TDPDKYTVVLDNERVRVLEYRDKPGQKTKMHTHPDFVLQALTPF
                     KRKLTLSNGKTMTREFKAGEIVWMNAQSHIGENIGETDTHVLITELKEIAGKKAVETG
                     VPNRIK"
     gene            398..1234
                     /locus_tag="VF089_17605"
     CDS             398..1234
                     /locus_tag="VF089_17605"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175831.1"
                     /translation="MVNKTEYEIDERDIEYQRQAGRPWLARIYQPQGAGPFPTLVDVH
                     GGAWHNGDRINNAGIDRALATRGVLVAAIDFRQPPEAGYPASICDVNLAVRWLKAHAA
                     EFNGTSGVGAFGNSSGGHQVVLSALRPRHPAYSALPLPKNPELDARLDYVIAGWPVID
                     PLYRFRFAKQFNREELIKAHIEYWKAEETMSEGSPQTIVEQDEQVDLPPILMLLKNND
                     RNHPLEMQERFIASYRKRGGAIEVHTFDALPEHRVVPSPSQPDTMRLIDTITEFISRK
                     AR"
     gene            1354..2505
                     /locus_tag="VF089_17610"
     CDS             1354..2505
                     /locus_tag="VF089_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175832.1"
                     /translation="MTAPTLDEIDRIARLSNPILRNLQITQCYYEISQSVAGFTGDSA
                     NWCTFATWASKQAGQTIRQEDLIRTFEERSYLSDKISAALEGIVSNLRRIGARLHTEV
                     PRKAMLRAINPGAAFARAGDAVARGNKKVFEEIGREFARFLEFFGEQTHFEPDKTARF
                     FAGLRAGEPPEGQRLLSQAFTSYCEARFQNGQEKAELMLLANLSAGFHEQIRLQPEIV
                     QALNAAIDNAAELKRNLMTVLLPDLYVRAREKVLRGQMTYVNAAFARLIEEVNRLIRQ
                     VITDHLMTLHLAGAEVLRLGRDLQGAFPQELGEIRNPKLREMLERIDITLNSLVESGA
                     KDWADFTDRMHFIADFFRVYQERQQLFEAPFTTEQVALLKRGAKPDGRL"
     gene            complement(2690..>3141)
                     /locus_tag="VF089_17615"
     CDS             complement(2690..>3141)
                     /locus_tag="VF089_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175833.1"
                     /translation="SIDALIESLQNFSGALLIVTHNQRILRALATRLVVFHRGRVDVF
                     NVGYDEFLEKVGWEGESDGKGAQKKPTSRNNYNEKKEREKADRREKARIAKLEAQIMK
                     SENALKEYHEQLVIEANRNNLAQMNHLSEKISQIKQEVDNLYREYAG"
BASE COUNT          707 a          833 c          881 g          720 t
ORIGIN      
        1 aaaccgatcc tgacaagtac acagttgtgc tcgacaatga acgggtgcgg gttcttgaat
       61 atcgggataa gccggggcaa aaaaccaaga tgcatacaca tcccgatttc gtcctgcagg
      121 ctctcacgcc gttcaagcga aagttaacat tgtccaatgg aaagacgatg actcgcgagt
      181 tcaaggcggg ggaaattgtc tggatgaatg ctcagagtca catcggtgag aatatcggcg
      241 agaccgacac tcacgtactg attaccgagt taaaggaaat tgccggcaaa aaagccgtcg
      301 agaccggtgt gcctaaccgg attaagtgag aatgttttac tacctggcgg cgattttgga
      361 cttgcagcgc cgtcccggcc ggctgtagat tgcagccatg gtgaacaaga ctgaatacga
      421 aatagacgag cgggatatcg aatatcagcg ccaggccggc aggccgtggc tggctcgcat
      481 ctatcagcct caaggcgccg gaccatttcc gacccttgtc gatgtccatg ggggcgcatg
      541 gcataacggc gatcggatca ataatgccgg cattgacaga gcgctggcga cccgcggcgt
      601 cctggtggct gctatcgatt ttcgccagcc gcccgaggct ggctatcccg cttctatctg
      661 cgacgtgaac ttagctgttc gctggctcaa ggcgcacgcg gctgagttca acggaacgag
      721 tggtgttgga gcgttcggca attccagcgg cggccatcaa gttgtgctca gtgcgctgcg
      781 gccgcggcac ccggcgtatt cggcgctgcc tttgccgaag aatcctgagc ttgatgccag
      841 gctggactat gtgatcgccg gctggcctgt gatcgatcca ctgtatcgct ttcgctttgc
      901 caagcagttc aatcgcgaag aactcatcaa ggcccatatc gagtactgga aggcagaaga
      961 aacgatgtcc gaaggcagtc cgcaaacgat tgtcgagcag gacgagcagg tcgatcttcc
     1021 gccgatactg atgttgctga agaacaatga ccgcaatcat ccccttgaaa tgcaggagcg
     1081 cttcatcgcc tcctatcgca aacgcggcgg cgccatcgag gtgcacacgt tcgatgctct
     1141 gcccgagcac cgggtggtgc cgtcgccaag ccagccggac acgatgcgtc tgatcgatac
     1201 catcaccgag tttatctctc gcaaggcgcg atgatgctgt ttgaggtagt gacccgtctg
     1261 ttagtcagcc gttgctgatg gcagcggaag ccacgcatca gctagcggca aagcttcgat
     1321 gatactttgc gccttaagct cgaagcagtt cctatgaccg cgccgaccct tgacgaaata
     1381 gatcgaatcg ctaggctcag caatcccatc ctgcgcaatc tacagattac ccagtgctac
     1441 tacgaaatct ctcagtctgt cgccgggttc accggcgact cggccaactg gtgcacgttt
     1501 gcgacgtggg cctccaagca agccggccaa accattcgcc aggaagattt gatccgaacc
     1561 tttgaggaac gctcttatct ctcggacaaa atttcagccg ctctggaggg gattgtcagc
     1621 aatctcaggc gcatcggtgc gaggttgcac actgaagtgc cgcgcaaggc gatgcttcga
     1681 gcgatcaatc cgggcgcagc attcgcgcgc gctggtgatg ccgtagctcg cggcaacaag
     1741 aaagtgttcg aggaaattgg ccgcgagttc gctcggtttc tcgagttctt tggcgaacag
     1801 acccacttcg agcccgacaa gaccgcgcgg ttcttcgccg ggttgcgtgc cggagagccg
     1861 ccagagggac agcgactcct gagccaggcc ttcacgagct actgcgaggc gcggtttcaa
     1921 aatgggcaag agaaagccga actgatgttg ctggcaaatc tttcggctgg ttttcatgag
     1981 cagattcggc ttcaacccga aattgtccag gcactcaatg cagcaatcga taatgccgcc
     2041 gagttgaaac gaaatcttat gaccgtcctg ctgcccgatc tttatgttcg tgcccgtgag
     2101 aaagtgctgc ggggtcagat gacctatgtg aacgccgcgt ttgcccggtt aatcgaagag
     2161 gtgaaccgtc tgattcgaca agtcatcacc gatcatttga tgacgcttca tttggccggt
     2221 gcggaggtgc ttcgactggg gcgcgatctg caaggagcat tccctcagga actgggggag
     2281 atccgcaatc ccaaattaag agaaatgctc gagcggatcg acataaccct gaatagcctg
     2341 gtcgaaagcg gcgcgaagga ttgggcggac ttcaccgatc gcatgcactt catcgccgat
     2401 ttcttccgcg tgtaccagga gcggcagcaa ctatttgaag cgccattcac gaccgagcaa
     2461 gtcgcgctgt taaagcgggg cgcgaaaccc gacggacggc tgtgacggaa tgtctcgcgt
     2521 actagcaggt tgatgaaaaa tgcggattgc agctgctcaa aagagttaga gccgaggcgc
     2581 gcgaaataca aggatgaaag cggaaatctg aaagcggaat tcatttttca gctttcataa
     2641 ttcatctttc caaaaggata tcaatccttt tggcagcctg ctagaagtct tatccggcat
     2701 actcgcgata caaattgtcg acttcctgct ttatctggct gattttctcg gacaggtggt
     2761 tcatctgagc gagattattg cgattggctt cgattaccag ttgctcatga tactctttta
     2821 atgcgttttc gcttttcatg atctgggctt cgagcttggc gattcttgcc ttttctctgc
     2881 ggtcggcttt ttcgcgttct tttttctcgt tgtaattgtt tcgcgaggtt ggcttcttct
     2941 gtgcgccttt gccgtcgctt tcgccttccc agccgacctt ttcgagaaac tcgtcatagc
     3001 cgacgttgaa gacgtcaacc ctgccgcggt gaaagacgac caatctcgtg gccagggcgc
     3061 gcaggatccg ttggttatga gtgacgatca ataatgcacc ggaaaagttt tgtaagctct
     3121 cgatcaaggc gtcaatggat t
//
LOCUS       DASUTG010000760         5180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2192439, whole genome shotgun sequence.
ACCESSION   DASUTG010000760 DASUTG010000000
VERSION     DASUTG010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..462
                     /locus_tag="VF089_17620"
     CDS             151..462
                     /locus_tag="VF089_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX6175834.1"
                     /translation="MTKAEIITRIYEKVGFSKKDATDVVEATFDIIKGCLEQGEKVKI
                     SGFGNFVVNTKRPRKGRNPQTGDEIIIVGRKVLTFKASQILKKSLNGMSSPTESPAET
                     E"
     gene            463..816
                     /locus_tag="VF089_17625"
     CDS             463..816
                     /locus_tag="VF089_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6175835.1"
                     /translation="MTTALPDKIYFKIGEVSEIVGVEPYVLRYWETEFDLLKPSKAPS
                     RHRLYKKKDVELLLDIKRLLYTEGFTIEGARKKLKEVKKEEKDQLKLPLSDQKYKTAL
                     AKIKKDLESLRRLLS"
     gene            1105..1181
                     /locus_tag="VF089_17630"
     tRNA            1105..1181
                     /locus_tag="VF089_17630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1139..1141,aa:Pro,seq:ggg)
     gene            1976..2893
                     /locus_tag="VF089_17635"
     CDS             1976..2893
                     /locus_tag="VF089_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX6175836.1"
                     /translation="MAFDQLDDFTAVILTGGKSSRMGRPKALLPFGKEPLIDHLVRRL
                     QHRFNLIVVVAAPDQELPPLPAKVVRDEIPYQGPVGGIYYGLKAAIGTGAFITSCDAA
                     FLNLPLISYLTSQILNHDVVVPYWQDRFQPLHAVYRKSVVPLLKEQLERGELRPIFLY
                     DKVRTRKVGEEEIRRFDPEGLSFFNMNVPDDYTQALMLWQQRENFTECTEDRSVSCTV
                     ELFGVARLLAKTQEVSLSLPPDATLTQVLSAVAQRLPVLVGRVIHPEEMDLLAGFTCN
                     INGTNFVRAPDTKIHRHDRIFILSADAGG"
     gene            2977..3411
                     /gene="trxC"
                     /locus_tag="VF089_17640"
     CDS             2977..3411
                     /gene="trxC"
                     /locus_tag="VF089_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX6175837.1"
                     /translation="MIRCTRCGATNRVPVEKLERRLQPVCGRCKAALPAVDNKPVAVT
                     DATFSAEVEHSPLPVLLDLWAPWCGPCRMVAPVLEELANEMAGRVRVAKVNVDENPAT
                     ASRFQVRSIPTLLVLKRGEELERIIGVQPKQEIVRRLERVIA"
     gene            3800..4414
                     /locus_tag="VF089_17645"
     CDS             3800..4414
                     /locus_tag="VF089_17645"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX6175838.1"
                     /translation="MVQDQLIARGIQDRRVIAAMRRIPRHLFIDPGLVSRAYDDSALP
                     IGQKQTLSQPYMAARMTEALGLSGKEKVLEIGTGSGYQTALLAELCFNVFSVERIRAL
                     SRKARELLDRLSFHNIALHVGDGTVGWSEHAPYDAIIVAASAPDLPRPLIEQLSVGGK
                     LIIPVGEDESQVLKLIRREADGFHQEDLGECRFVKLWGKFGWQD"
     gene            4402..5166
                     /locus_tag="VF089_17650"
     CDS             4402..5166
                     /locus_tag="VF089_17650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6175839.1"
                     /translation="MARLGAGSPYRLLGLMLFWHIAGCATQAPAPVATRGGVYHVVKS
                     GETLYRIGRAYDLNYAELARVNRLRDPNEIRVGQRLFIPGANRQLPVEMITPSDSNAR
                     IPALSRSSDLTATRIIWPIVGGINSHFGRRGVTFHDGIDISAEEGTPIRAIRQGEVIY
                     SDQLRGYGNLVIVRHSEGLVSVYAHNQVNLVTAGQFVAQGDIIAKVGSTGRVTGPHLH
                     LEIRKNNVAQDPLLYLPQLCCRESSDIVDAGKSIME"
BASE COUNT         1262 a         1309 c         1376 g         1233 t
ORIGIN      
        1 ggcgtacaaa attttctatc gtgcggatga tcggacatta acggacagcg aggttaatac
       61 gatgcatgaa caggtgctcg atcatgttgg caagcttttt caggcgcaaa ggcgaagcta
      121 aaccgaactg cgagggaagg aatggctgca atgaccaagg cggaaatcat cacgcgaatc
      181 tatgagaaag tcggcttctc aaaaaaggat gcgaccgatg ttgttgaggc cactttcgac
      241 atcattaaag gttgtctcga gcaaggcgaa aaagttaaga tctccgggtt cggcaatttc
      301 gttgtcaaca cgaagcgacc gcgtaaggga agaaatccgc aaaccggtga tgaaattatc
      361 attgtcggac gtaaagtcct cacatttaag gccagtcaga ttttgaagaa gagcctgaat
      421 ggcatgtcat caccgacgga atcaccggct gaaactgaat agatgaccac agcattaccc
      481 gataagatct attttaagat cggcgaggtc agcgaaattg tcggtgttga gccctacgtg
      541 ctgcgttact gggaaactga atttgatctg ctcaagccgt caaaggcgcc ttcacgccat
      601 cgtctctata agaaaaaaga cgtcgagctt ctgctagaca tcaagcgcct tctctatacc
      661 gaaggcttta cgattgaagg cgcccgtaag aaattgaagg aagttaaaaa agaagaaaaa
      721 gatcagctga agcttccgct ttccgatcag aagtacaaaa ccgctctagc aaagatcaag
      781 aaagatctcg aatcgcttcg acgtctactt tcctgaagcg aagtcgaagt ccatcgtctc
      841 gcaatcagcc ggagatccgg tccatacaac ggttgtgcaa cttggcagcc gcaggttaag
      901 agactagctc ctcgtgggca atctttcacg gccgccccga ctaacctgca cgactcaacg
      961 caatgatata ccccttcccc tccgatgggc ggcttccgcc cggataagct tctaggaaat
     1021 cctttcaagt tcagaaacgg cctggcacta aattgctttg gcgaatatcg agtgttaaag
     1081 tcacgcctaa tttaaacact ttatcggggc gtagcgcagc ctggtagcgc acacgcttgg
     1141 ggtgcgtgtg gtcaccggtt caaatccggt cgccccgacc attctttttc ggtttttcat
     1201 cgccgccaga tccttcttaa acttaggtcc gccgctaagc atcagcacat cacatcctga
     1261 agttagttgt tcccggattt tggaggcatt tctggttttt cgaatgcgcc ctgcggggct
     1321 cacagcagaa gagcttctct tcaccagatc tcgggcattg acctcctaaa taatttagtc
     1381 gctgtttcga cacatactcg gtgatcatac tcaagttatg aagcgttttt gcatcaaatg
     1441 ttctcaaaca gtaggccgat ggctgagcac gaatgtgtaa agcatcacgg gtggatcttc
     1501 ttctatgagc gattctggac tgattacctg cctccggcac gaagtttgct gcgccctacg
     1561 attatgccgg aggatctgct atgacttcga aacagcatct gacttctgca catacttccg
     1621 aagctcctga tgcagtcgag ttagctcaca tcaattcgga attgtgttgg tgtgatccga
     1681 ctctggattt cgatgaggac ggaaaccagt ctttaactca caacgaggtt acctggaact
     1741 agatccgtta tgtctgtcgc gtaaccattc acgcatcgtc aggtgatcta attccgtctg
     1801 caaacctgcc atgcccgttt caaatcattt gcaattatct aaagtaaagc caagcgcgcg
     1861 gttttctcac ttaagaaata atcaaacctt agcgattttg taagcacagg attgtaagca
     1921 cagacggtcg ccggagagac aatggcgtga taagaattgt attgggaggg taacgttggc
     1981 cttcgaccaa ttggacgatt tcacggcggt gattttgacc ggcggcaaaa gctcgcgcat
     2041 gggtcgtccg aaagctttgc tgccgttcgg caaggagcct ttaatcgacc atctcgtgcg
     2101 caggctccag cacagattca atctcatcgt ggtggtggcg gcgccggacc aggagctgcc
     2161 gcccttaccc gcaaaggtgg ttcgcgatga gataccttac caaggaccag taggcggtat
     2221 ttattatggt ttgaaagcgg cgatcggaac cggtgccttc attacctcgt gcgatgcggc
     2281 gtttctaaat ttaccactta tctcgtattt gacctcgcaa attctaaatc acgacgtcgt
     2341 cgttccctat tggcaggatc gatttcagcc tcttcacgct gtttaccgaa agagcgtagt
     2401 gccgctgcta aaagagcaac tcgaacgcgg tgagcttagg ccgatatttc tttacgacaa
     2461 agtgcggacg cgtaaggtcg gtgaggagga aatccgccgc ttcgacccag aagggttaag
     2521 cttcttcaac atgaacgtgc ctgacgacta cacacaggct ttgatgctat ggcagcagcg
     2581 cgagaacttt accgagtgca ccgaagaccg gtccgttagt tgcacggttg aattgttcgg
     2641 cgttgcccgc ttacttgcga agacccaaga agtatctctt tcgctgccac ctgatgccac
     2701 tctcactcaa gtgctgtcgg ccgttgcgca gaggttgccg gtcttggttg gccgtgtcat
     2761 tcatccggag gagatggatt tgctcgcagg gtttacttgc aacataaacg gtactaattt
     2821 tgtgcgcgct cctgacacca agattcaccg gcacgaccga atctttattc tctctgccga
     2881 tgccggcgga tgatcaaatt tttttctgtt tttgcgagca cagctgtctt gagtaaggaa
     2941 taacctagaa ggtcctacaa gatgcctgat tcgctgatga tccgttgcac tcgctgcggg
     3001 gcgacaaatc gagttcctgt ggaaaaactc gaacgaagac ttcagccggt gtgcggacgt
     3061 tgcaaagcgg cactgccggc ggtcgataac aagccagtgg ccgttaccga tgcaacgttc
     3121 tcagctgaag ttgagcattc gcctctgccg gtcttgctgg atctatgggc gccatggtgt
     3181 ggcccatgcc gtatggttgc gccagttcta gaagagctgg ccaatgaaat ggcgggtcgt
     3241 gtgcgagtgg caaaggtgaa tgtggatgag aatccggcaa ccgccagtcg ttttcaggtg
     3301 cgcagtattc ccactcttct ggtactcaaa agaggcgaag agctggagcg catcatcggt
     3361 gtccagccga aacaagagat agtacgccgg ttggaacgag ttatagcgtg accccgtgcg
     3421 gggactgtga gacatccgat tttgtggcgg aatcagacaa gcgggatcat gaagctttgg
     3481 gcaaatcgga cgacagagaa ctcttcggct ccatcctcgt caactgcgca gtggcaatca
     3541 actcggcgat acgatgtctt cttcgctgag cgatttcata gaaaacctta tccctctatg
     3601 gtaaagatat cgccgtgact ggtcgcaaaa tcagcagcaa aaactccgca accgacgtcg
     3661 gagcagccat tttgcgatct gccagtatat gcttgactga ctgatcacgg ttgctgaagc
     3721 cgccgatcga gcgaccaaac acaaacgcgt gaagcttacc gaaacgacac ctccggatta
     3781 cgcttgggcc agagaaagaa tggttcaaga tcaacttatc gcccgtggaa ttcaggaccg
     3841 ccgcgtcatc gcggccatgc gacgcattcc aagacatctt tttatcgacc ccgggctggt
     3901 cagccgggct tacgatgata gcgccctccc gatcgggcag aaacagacgc tctctcaacc
     3961 ttacatggcc gcgagaatga ccgaagcgct gggtctgagc ggtaaagaaa aagtcctgga
     4021 aatcggtacc ggatcagggt accaaacagc tctgcttgcc gagctctgct tcaacgtatt
     4081 ctcggtggag agaatccgcg ctctctcgcg caaagcaagg gagcttctag accggctttc
     4141 atttcataac atcgcactac acgtgggaga tggaacagta ggttggtcgg agcacgcccc
     4201 ttacgatgcg atcatcgtcg ctgctagcgc tcccgatctg ccccggccgt taatcgagca
     4261 gctttcggta gggggtaagc tcattatacc ggttggggag gatgaatctc aggtgctaaa
     4321 gctgattcga cgtgaagcgg acggttttca tcaagaagat cttggcgaat gccgatttgt
     4381 caaactctgg gggaaattcg gttggcaaga ctaggcgccg gatctcctta ccggctactt
     4441 ggcttgatgt tgttctggca tatcgctggt tgcgcgaccc aggcgccagc gccggtggct
     4501 actagaggag gcgtctatca cgtcgttaag tccggtgaaa ctttatatcg catcggcagg
     4561 gcttatgatc tgaactacgc ggaactggcg cgtgttaatc gactcagaga tccgaacgaa
     4621 atccgtgtcg ggcagagact atttataccg ggcgcgaacc gccaattgcc ggtagagatg
     4681 atcaccccga gcgattcgaa tgcaagaatt ccggcgctct ccaggtccag cgatctgacc
     4741 gcgactcgca ttatctggcc cattgtcgga ggcatcaatt cccattttgg ccggcgcggc
     4801 gttactttcc acgacggtat cgatatctcg gccgaggaag gaactcccat tagggcgatc
     4861 cgtcagggcg aggtgatcta tagtgatcaa ctgcgcggct acggtaactt ggtgatcgta
     4921 cggcactccg aaggcctggt ttcggtctat gcgcacaatc aggtaaatct tgtgactgca
     4981 ggacagtttg ttgcccaagg cgatatcatc gctaaagtcg gtagcacagg gagggtgacg
     5041 ggacctcatc tgcatcttga gatccgcaag aacaacgtgg cgcaagatcc actcctgtac
     5101 cttcctcagt tgtgctgccg cgagtcgtcc gatatcgtgg atgcgggaaa gtcgattatg
     5161 gagtgatgtc aatgaaggaa
//
LOCUS       DASUTG010000761         7260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_856957, whole genome shotgun sequence.
ACCESSION   DASUTG010000761 DASUTG010000000
VERSION     DASUTG010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856957"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1052)
                     /locus_tag="VF089_17655"
     CDS             complement(240..1052)
                     /locus_tag="VF089_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6175840.1"
                     /translation="MNLEVYLEHMTDALKRHWPEYLMEAACLGLFMISAFTFGAILEH
                     PVSPLHQAIPYSLLRRFLMGVAMGSTAILIIYSPWGKQSGAHINPSTTLTFFRLGKVA
                     KWDAVFYIVAQFGGAVTGALLASAVLAPSVSHPSVNYVITMPGSAGIAAAFIAEVAIS
                     FILMSVILHVSNNTRLHKLTGVCAGALVAAYITLEAPISGMSMNPARTFASAVPAQYW
                     TSLWLYFTAPLIGMLAAAEAYLRSHSPDIGCAKLHHQNSKRCIFCGKPASEA"
     gene            complement(1150..2262)
                     /locus_tag="VF089_17660"
     CDS             complement(1150..2262)
                     /locus_tag="VF089_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6175841.1"
                     /translation="MKAIAVTPRKKTVEIINQAEPQIFSPTDVKLRMLEAGVCGTDRE
                     ICAFEYGTPPAGCEQLVIGHESLGEVIETGPKVTRVKAGDLVVPMVRRPCPHDDCVAC
                     RADRQDFCFTGEFQERGIKERHGFMAQFVVDDEKYMNPVPREMRDVAILVEPLTIAEK
                     GLTQVWQMQQRLPWGCPVTPAKAAAHCHRAVVLGAGPVGLLGAMALINYDFDTYVYSR
                     EPAPNPKSRLLESIGAHYISSENIAPEMLPEQIGNVDLIYEATGASSLAFETMKYLGT
                     NGIFIFTGVPGRKRPIQVDTDLIMRNLVLKNQVVFGTVNAGRDSFENSIRDLGTFAKR
                     WPEAVQSLITGRFPMEAYGDLLLGRSGGIKNVIRLN"
     gene            complement(2608..2928)
                     /locus_tag="VF089_17665"
     CDS             complement(2608..2928)
                     /locus_tag="VF089_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6175842.1"
                     /translation="MKRHRSSPSRKRIGSSNNNKTGESRFRPLTCEKETALIADYLAS
                     NLGRADLASFENHLEECPDCKAFLQTYKKTIEITRSFFELESAKARQRKPARRAANSR
                     QRRN"
     gene            complement(3000..3236)
                     /locus_tag="VF089_17670"
     CDS             complement(3000..3236)
                     /locus_tag="VF089_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175843.1"
                     /translation="MKKTRTPYIQFPPKKSPIQTTFMDLIQELSQLTNDDGMVVAAVK
                     RVFNSHTVRFAHSLTPVRLVAGGRTLKSGIRTRR"
     gene            complement(3325..4086)
                     /locus_tag="VF089_17675"
     CDS             complement(3325..4086)
                     /locus_tag="VF089_17675"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6175844.1"
                     /translation="MQDSFVSTEIDRRNSMASCNSMMELGYTSDTRKTGSATGRLESW
                     SSSNASRRKKTSDKDDPALIERLKAGDHEALETIVDLYSPKLYNVAQRILGDSADTEE
                     VIQDVFWTAFRKAKTFQGNSRFSTWLYRLTVNEALGKIRRRKNHKREIEYEEYLPKFT
                     DDGHHRIRPVVDWSDTLDRSYVEREIQGVVKAALDGLKPLDRSVVVLSDLEGMSDKEI
                     ATIAHLTVSAVKTRLHRARLFLRGRLATYLGHSAE"
     gene            4577..6967
                     /locus_tag="VF089_17680"
     CDS             4577..6967
                     /locus_tag="VF089_17680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6175845.1"
                     /translation="MIVGTIEPNRGDAELGDSEDPFWLLFENSNDAVLLSGADGIIEA
                     ANPAACRLFGWAEEALRQIGLTALVDPADGRLSVLLKEQEQTGRFKAELNFKRKDGSV
                     FLGEVCSGFYKDNVGIGKATFIIRDISERQPIEETLRHITEGTAGSTGDEFFRSLVKH
                     VSQALQVRYCFVAECADETDKRVRMLAFWAGQGFGENIEFPLAGTPCERVIQGNICSY
                     PERLQVLFPADPGLIALGAQSYAGVPLMNSAGSILGHLVVIDTQARVFSEQALSILQV
                     FATRAAVELERKRADDALKKSEEHLRTLLGINNAVVTKLTRDELFCAIAEVLGRAIPF
                     DRLSLSLYDPAAAVLRLVTYAGPYQRDDYTPIGRVLELHDSPAGMAFVSQEPILRSDL
                     EAQRQTSSEERAYGHGFRSVCALPLIVRGKSIGAITVGSLTKNRYAEGDAQFLMEVAN
                     QIAIAVDNMRSYEEIDALKGRFQSEAVYLQEEIKLEHNFEEIIGQSAPMRQLLRTIEQ
                     VAPTEATVLIQGETGTGKELLARAVHNRSLRKDRPLVKVNCGSIPSGLVESELFGHEK
                     GAFTGATQRRIGRFELANGGTIFLDEVTELPFDTQVKLLRVLQEGEFERVGSSETIKV
                     DVRVIAATNRELKEVVANGTFRSDLFYRLNVFPLKAPPLRERKGDVLLLVHFFLGKYA
                     KKLGKEVRSVSQKSMESLTNYNWPGNVRELQNVVERAVVLASGSIANIDDSVLQSHST
                     VQQSIDTLENIERHHIVRALNETGWVIHGKKGAAEILGINPSTLRSRMDKLGIRRP"
BASE COUNT         1627 a         1888 c         2043 g         1702 t
ORIGIN      
        1 gcatttacgc tgagcttcat caatttcctc caaaggtttg gatatttttg gcttgctgct
       61 tagatgcgac tgccatcaga aaagatgcgt cgagccgtgc gcagcagctc gatccaagcc
      121 aatttagtga agtctttgcg gatccaggcg agtgtcgctc cggctaatct ttaaatgcct
      181 ggcgtgagaa gaatactgca gccgtcccgc taccgcgcgg ataattgcac acctagtagc
      241 tatgcttcag aggcgggttt gccgcagaaa atacaacgct tcgagttttg gtggtgcagt
      301 ttggcgcagc cgatatccgg actgtggctt cgcaggtaag cttcggcggc ggctagcatt
      361 ccgatcaaag gagcagtgaa gtaaagccat agcgacgtcc aatactgcgc cggcactgcg
      421 gaagcaaagg tgcgcgcggg attcatactc attcccgaga tcggcgcttc aagcgttatg
      481 taagccgcga ccaacgctcc tgcgcaaacg cccgtgagtt tgtgaaggcg ggtgttgttt
      541 gacacgtgga gaatcacact catcagaatg aaactaatcg ctacttcggc gatgaatgcg
      601 gcggcaattc cagcgctccc cggcatcgtg atgacgtaat tgaccgacgg atgggagacc
      661 gatggagcca gaacagctga ggccagaaga gcaccggtta ctgcgccgcc aaactgggcg
      721 acgatgtaga agaccgcatc ccatttagcg accttgccga gtcgaaaaaa cgtcagggtg
      781 gttgacgggt tgatgtgggc gccggattgt ttgccccaag gtgaatagat gatgaggata
      841 gcggtcgagc ccatcgcgac ccccatcaag aatcgtcgca gcagtgagta gggaatagct
      901 tggtggaggg gcgacacagg atgttccaaa atcgcgccga aggtgaaggc cgagatcata
      961 aacaggccga ggcaagcggc ctccatcaga tattcgggcc aatggcgttt caaggcgtcc
     1021 gtcatgtgct ctaagtagac ttcaaggttc accgagttcc acgtcgggat catccgaaac
     1081 ggtgcgtcgt taaagattgc tctgctcttc aaagttcccg ctgcgtgctt ctaacaacca
     1141 tccgccggat taattgagcc ggatgacatt cttgatccct ccggagcggc cgagaagaag
     1201 atcgccgtaa gcctccattg ggaagcgccc ggtgatcagc gactgcaccg cctcgggcca
     1261 acgtttggca aaagtgccga gatcgcggat agagttttcg aaggagtcgc gcccggcatt
     1321 gaccgtgccg aagacgacct ggtttttcag aaccagattg cgcatgatga ggtcggtatc
     1381 gacttgaatg ggccgttttc tgccgggcac gcctgtgaaa ataaagatgc cgttggtgcc
     1441 gaggtacttc atcgtttcga aggccaggct cgaagctccc gtcgcttcgt agattaaatc
     1501 cacgttgccg atctgttcgg gaagcatttc gggagcgatg ttctcagacg aaatatagtg
     1561 cgcgccgatc gattcgagta gtcgagactt cggattcgga gcgggctctc gcgaataaac
     1621 atacgtgtca aaatcgtaat tgatcaaggc catggcccca agcagtccca ccggtcccgc
     1681 gcccaggaca acagcccggt ggcaatgagc cgctgctttt gccggtgtga ccgggcagcc
     1741 ccaaggcaag cgctgttgca tttgccatac ttgagtcagc cccttttcag caatcgtcag
     1801 cggctctacc agaatggcaa cgtcacgcat ttccctggga acggggttca tgtacttttc
     1861 gtcgtcaacg acgaactgcg ccatgaaccc gtgcctttct ttaatacccc gttcttgaaa
     1921 ttctccggtg aaacaaaaat cctgacgatc ggcgcggcac gccacgcaat cgtcgtgcgg
     1981 gcacggccgg cgaaccattg ggacgacgag gtctcctgcc ttcacgcggg taactttcgg
     2041 acctgtttca attacctcgc cgagagattc gtggccgatg acgagttgct cgcagccggc
     2101 gggcggcgtg ccgtattcga aggcacagat ttctcggtcc gtgccgcaga ctccggcctc
     2161 cagcatgcgc agcttgacat ccgtggggga gaaaatctgc ggttcggctt gattgataat
     2221 ttcgacggtt ttttttctcg gcgtaacggc tatagctttc atgcacgaag ctccttgaaa
     2281 acggtgaatg atcgacgtta gcgaaccgga aggttcagcc cggattccgt agttaaaaac
     2341 tgagcgaggg gcaagcctgg agaagcgaag acaccggagt gttccgtttc aaagtccttt
     2401 ttgatgcttg gctcgccgcc tctatttgcg tgacgtcgtt acgcatggca ccgacagttg
     2461 tcgcctctca gaggtagaaa cgcattgtct gcgctgcgga aggtttgctc ctcgctcatg
     2521 acttctcctt caactgcgga atccgggttc agtagaatcc tgtgcacatt tggatgaggg
     2581 tcgatgggaa aaggatgcac agattattta gttccggcgc tgccggctgt tcgcggcgcg
     2641 ccgcgccggt ttgcgctggc gcgctttggc agattcaagc tcgaaaaagc tgcgggtgat
     2701 ttcgatagtt ttcttgtagg tctgcaaaaa cgccttgcaa tcggggcact cttcgagatg
     2761 attttcgaac gacgcgaggt ctgcgcggcc gagattcgag gcgagatagt ctgcgatcag
     2821 ggcggtttct ttttcgcagg tcagcggccg aaatcggctt tcgcccgtct tgttgttgtt
     2881 tgacgagccg atccgttttc tgctcggaga ggagcgatgg cgtttcatcg tcctatttat
     2941 ctcgacagcg gcaataattt gcaaatcctc caatcgatgc ggcggccggc atcggcagct
     3001 caccggcgcg tacggatgcc tgacttcaag gttctcccgc cggccaccag gcgcacaggt
     3061 gtgagcgagt gagcgaatcg tacggtatgg gaattgaaga ctcttttaac cgccgcgacg
     3121 accatgccgt cgtcgttggt gagttggctg agttcctgga tcaggtccat aaaagtagtc
     3181 tgaatgggac tctttttcgg cggaaactgt atgtacgggg ttcgggtttt tttcatgatc
     3241 atgcctccga tgtaatctca ggcttgtttt agatgctcgt ctgtcgacaa aggttgcaag
     3301 agcttcaaat ttcagcggtc gccattattc ggccgagtgg ccgagatagg tggctaatcg
     3361 gccgcgcaaa aaaagccggg cgcgatgaag tcgggtcttt actgcagaca cagttagatg
     3421 cgcgatagtc gcgatctctt tgtccgacat gccttccaaa tcgctgagta cgacgacgct
     3481 gcggtcgagg ggtttcaatc catccaaagc agctttaacg acaccctgta tttctcgttc
     3541 gacataactc cgatcgagag tatcggacca gtcgaccacg ggacggatcc gatggtgacc
     3601 atcgtcggta aacttaggca ggtattcttc gtactcgatc tctcttttat gatttttgcg
     3661 acgccggatt tttcccaaag cctcgtttac ggtcaagcga taaagccatg ttgaaaaccg
     3721 ggagttgccc tggaaagtct tggccttgcg aaaggcggtc cagaagacgt cttggatgac
     3781 ttcctcggta tccgcgctgt cgccgagaat tcgttgcgct acgttgtaga gctttggtga
     3841 atagagatcg acgatggtct caagcgcctc gtgatcaccg gctttaagcc gctcgatcag
     3901 cgccggatcg tctttatcgg aggtcttttt ccttctggag gcgttggaac tgctccacga
     3961 ttcgagccta ccggtcgccg accctgtctt tctcgtgtcg ctggtgtacc ccaactccat
     4021 catggaattg caagaagcca tagagtttct tctatcgatt tcggtgctga caaatgaatc
     4081 ttgcatgagt tgatctccta tgttgctacg gttcttaaga gcatttcgac aaacggggag
     4141 caattgataa tgctcagccg attgctgcgt aattccacca atctttttgc aacttcttcg
     4201 tttatgaaaa ccacgccggc catgtccaag tgaattatct tcccgtcact ctcctgatat
     4261 tcggagcaga gtgcttctag ctccccgcac gttgtcgctg ttagggtgcc gtccaaacgt
     4321 aaactgaccg tttcctcatc tggagagctt tgagtaattc gcagcataga gcttgtgact
     4381 cctaaaactg ttgtgatctt agagcacagg tgatgccatc tatgtaaccg gttgattctg
     4441 ttgagaagcg ttccagaaac ggatcttacc gcgagccgac ctatcgtggc atttcgcaat
     4501 gcttgcgaaa tgtcgcggcc cgtcgtaata cgcagaacaa gtgttatgcg agagactacg
     4561 gttaggatga tccactatga tcgttggcac gattgagccg aacaggggtg atgcggaatt
     4621 gggcgatagc gaagatccgt tctggttgct gtttgagaac agcaacgatg cggttttact
     4681 ttctggcgcc gacgggatca tcgaagccgc caatcccgcg gcatgccggc tgttcggctg
     4741 ggccgaggag gcgcttcgcc aaatcggcct caccgcgctg gtggatcccg ctgatggtcg
     4801 attgtcggtg ttgctaaaag agcaggagca aaccggccgc ttcaaagccg agctcaattt
     4861 caagcgcaag gatggctcgg tttttcttgg cgaagtttgc agtggttttt ataaggacaa
     4921 tgtcggaatc ggaaaagcca ctttcataat tcgcgacatc agcgagcgcc agcctataga
     4981 agaaactctc cggcacatca ccgagggaac ggccggcagc acgggggatg aattctttcg
     5041 ttcgttggtc aaacacgtgt ctcaagcgct tcaggtccgc tactgttttg tcgccgaatg
     5101 tgcagatgag actgacaaac gagttcgtat gttggctttt tgggcagggc aaggctttgg
     5161 tgaaaatatt gaatttcctc tcgccggcac gccttgcgaa cgcgtcatcc aaggaaatat
     5221 ctgttcctac ccggagcgat tacaagttct ctttccggcg gatccaggac tcatcgcact
     5281 gggggcgcag agttatgcgg gcgttccgct catgaattca gccggaagta ttttagggca
     5341 tctggtcgtg atcgacaccc aagccagagt tttcagcgaa caggctctat cgatcctgca
     5401 agtattcgcc acccgggctg cagtcgagct ggagcgaaaa cgtgccgacg acgcgttgaa
     5461 aaagagcgaa gagcatctcc gcacgctgct cggcatcaac aatgctgttg tcactaaatt
     5521 aacgcgagac gagcttttct gcgccatcgc agaggtcctc ggccgggcga ttccgttcga
     5581 tcgactctca ctttcactct acgacccagc ggccgctgtc ctgcgactgg tcacttacgc
     5641 cggcccatac cagcgcgacg attacacccc catcggtcgt gtgcttgagt tgcacgatag
     5701 ccccgccggg atggcattcg taagtcaaga acccattctg cgcagtgacc ttgaagccca
     5761 gcggcagact tcctcggagg aacgcgcgta tgggcacggg tttcgctccg tatgtgctct
     5821 gccgcttatt gttcgcggaa agagtatcgg cgctatcact gtaggaagtc tgacaaagaa
     5881 ccgttacgcc gaaggggacg ctcagttcct catggaggtt gccaatcaaa tcgctatcgc
     5941 cgtcgacaat atgagatctt atgaagaaat cgacgcgctg aaagggagat ttcaatccga
     6001 agccgtttac ctccaggagg aaataaagct tgagcataac tttgaagaga tcatcggaca
     6061 gagcgcgccc atgcgtcagt tgctgcgcac catcgaacag gtagcgccca ccgaagcaac
     6121 cgtattgatt caaggtgaga cgggcaccgg caaagaactc ctggcgcgag cggttcacaa
     6181 tcgaagcctg cgcaaagatc ggccgctggt caaagtcaac tgcggatcca taccgtccgg
     6241 cctggtcgag agcgagctct tcggccatga gaaaggcgca ttcacgggag caacacagcg
     6301 ccgcatcggc aggttcgaac tggccaatgg cggaaccatc tttcttgatg aggttacgga
     6361 attacctttc gatacccaag tgaaattgct tcgcgtgctg caggaagggg aatttgaacg
     6421 ggtgggtagc agcgaaacaa tcaaggtcga tgtgcgagtg atcgccgcca ccaatcgtga
     6481 gctcaaggag gtcgttgcca acggcacgtt ccgatccgat cttttttacc ggctcaatgt
     6541 atttcctctg aaggccccgc cgttaaggga gcgcaaggga gacgtgctgc tcctggtcca
     6601 cttttttcta ggaaaatatg ccaagaagct cggcaaagaa gttcgcagcg tttcacaaaa
     6661 gtcgatggag agcctcacga actacaactg gccgggaaac gtgcgggaat tgcaaaacgt
     6721 cgtcgagcgg gcggtggttc tcgccagcgg ttcgatcgca aatatcgatg attcggtgct
     6781 tcagtctcat agtactgtcc aacagtcgat tgatacgctt gaaaacatcg agcgccatca
     6841 catcgtccgg gctttgaacg agaccggttg ggtgatccat ggcaagaagg gcgcggccga
     6901 gatcctcgga ataaatccca gcacgttgcg gtcgcggatg gacaaactcg gaattagacg
     6961 accctagcat ttgatcagcg caggttccaa aattggcgct tagcacatca gcctttgatt
     7021 caatgcagcg agatcacata ttctgccttc agaatattat cttgcaacat tgtgctagcc
     7081 ctaatcgata ataactcacg tcactatggt cagtgaggca tcgacgctcg aaaccgtgag
     7141 caggtaaacg gacaggttgt ctgcgcacga gcgatctgtc acatcacccg cgatcgcttg
     7201 gggagtggtc cttgcttgca gcctcgcgtg cgaagcggtc tggaaaaatg tcggttcggt
//
LOCUS       DASUTG010000762         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1007573, whole genome shotgun sequence.
ACCESSION   DASUTG010000762 DASUTG010000000
VERSION     DASUTG010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007573"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF089_17685"
     CDS             <1..409
                     /locus_tag="VF089_17685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6175846.1"
                     /translation="DQLQRRFASHVCLMLAEGDGKIIAGTFNIRDHTAMYGRYWGAFH
                     DHPFLHFNVCYYSAIEHCINMGLQRFEAGAGGSFKQLRGLDPEHTTSMHYIVDGRFRR
                     AVERFLKHERELIRQKRDLLLERSQLKRELEEP"
     gene            619..1395
                     /locus_tag="VF089_17690"
     CDS             619..1395
                     /locus_tag="VF089_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175847.1"
                     /translation="MKASTRNIIEPIVFFLALLVLWEILVEIIKVPSFILPPPRDLWI
                     AFVNKLPIIGHHAWITFIEAFGGFALSLLLGIGFAVAVVYSRHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGLMSKIAIAFLVAFFPIVVNTVVGLKEVEPELLELARINRGSQ
                     FDIFKKIRLPNSLPYMFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYSLDTAF
                     ALVILLILAGLSLGLFWLVEIIQRKLAPWSAEVRQVEGSF"
     gene            1414..2436
                     /locus_tag="VF089_17695"
     CDS             1414..2436
                     /locus_tag="VF089_17695"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175848.1"
                     /translation="MTEFGNTFSIGRIAAVILNAIMAMTIFTFNAVAAQPTPANIRMD
                     FIIGGKHAPWYVALEKGFYAKRGLAATIQPGSGSADTVRNIAAGGADFGFADISTAIV
                     ARSRGTGVQVVGQLGYVAATILWREDSGIKGIKDLQGKSWAISPGQAQWFLMPAYCRI
                     NGIDFKSIKIQETAAPIQPAALATKKADFIIMFRASNDEVAELAASKVGSKLNRVFMK
                     DTGLDIYGSGLVAKEEDIKKRPDFVRAYVEGTMEGLRYTRDNQEEALQILLKQKPELD
                     KALATLQLRNALYEVMLPPESVQSGLGYMKPDIMEKTVRVTNEYFDVARKVTSKEVFT
                     NQFIKR"
     gene            2623..>2909
                     /locus_tag="VF089_17700"
     CDS             2623..>2909
                     /locus_tag="VF089_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6175849.1"
                     /translation="MPSLIAVDNVCRVFTSGARMVNALDRISFEIHTGEFVSIVGPSG
                     CGKSTLLKILSGLLPASSGTIVMSGKAVDEPVENVGMVFQSPVLLKWRPVLG"
BASE COUNT          642 a          720 c          804 g          743 t
ORIGIN      
        1 tgaccaactt caacgaaggt tcgcgagcca tgtttgcttg atgttggccg aaggcgacgg
       61 caagattatc gccggaacct tcaacatcag agatcatacc gctatgtacg gacgatactg
      121 gggtgccttc catgaccacc ccttcctcca tttcaatgtc tgttactact cagcaatcga
      181 gcattgcatc aacatgggcc tgcagcggtt cgaagccgga gccggcggca gcttcaaaca
      241 acttcgcggt ctagatcccg agcacacgac cagcatgcat tatattgtcg acggtagatt
      301 tcgtcgtgcc gtcgagcgct tcctaaaaca cgagcgggag ctaattcgcc aaaaacgcga
      361 tctactgctt gagcgcagcc agctgaagcg cgagctggaa gagccttagc aagctgctga
      421 agggcctcat atgctttgtt gcgctggctc cgagtcgctt caacgtagtg tgccctagtc
      481 tccgctcatc gaacatcgca cgcgcgcatc tgagtctttc tgagcagcct gcaatgagaa
      541 tcttagccac tgttagtgcg cgccgtttcc ttgacagggt aggagctttc ctttaacgtc
      601 ggcttcggag ttggggacgt gaaggctagc acgaggaaca tcatcgaacc tatagttttc
      661 ttcctggcgc tcctagttct ttgggaaatt ctggtcgaga tcatcaaggt tccttccttc
      721 attctgccgc cgccgcggga tctgtggatt gcgtttgtaa acaagttgcc gattatcggg
      781 catcatgcct ggatcacttt catcgaagct tttggcggat ttgccttgtc tctactgttg
      841 gggattggtt tcgctgtcgc ggtggtctac tcgcgccatc tacagaatac gatctatccg
      901 ctcatcgtga ttctttatgc gatgccaaaa agcgcgttcg cgccgttgat ggtcatatgg
      961 gttggctatg ggcttatgtc taagatagct attgcgtttc tcgtcgcgtt ttttcccatc
     1021 gtggtgaata cggtggtcgg gttaaaagaa gttgagcctg aattgttgga gttggcacgg
     1081 atcaaccgcg gttctcaatt tgacatcttc aaaaagatcc gtctgcctaa ctcgctaccg
     1141 tatatgttcg ccgggatcaa agtggccatc gtgctttcgg tcactggcgc aattgtcgcc
     1201 gagttcgtcg ctgccaacga aggtttgggc tatctagttt tgcaagcgaa ttacagtttg
     1261 gatacagcat ttgccctggt aattctgctg attctagcgg ggctgtcgct gggacttttt
     1321 tggctggttg aaattatcca acgaaagttg gcgccctgga gcgctgaggt gcgtcaggtg
     1381 gaaggttctt tttgatatcg gagggtgggg cacatgacgg agtttggcaa tacgtttagc
     1441 atcggcagga tagccgcggt gatcttaaac gcgatcatgg ctatgaccat tttcaccttt
     1501 aacgccgtag ccgcgcaacc gacaccggcc aacattagaa tggacttcat catcggcggc
     1561 aaacatgcgc catggtacgt cgcgttggag aaaggttttt acgccaaacg cggccttgcc
     1621 gcgacgattc agcccggctc gggctcggcg gatacagtac gcaacattgc ggccggcggc
     1681 gctgattttg gtttcgccga tatttcgacg gctattgtcg cacgatcccg cgggaccggg
     1741 gttcaggtcg taggccagct tggttatgtc gccgccacca tcttgtggcg cgaagattct
     1801 gggataaaag ggatcaagga cctgcaaggc aagtcatggg ccatcagccc cgggcaggcg
     1861 cagtggttct tgatgccggc ttactgtcga atcaacggca tcgatttcaa atcgatcaag
     1921 attcaggaga ccgcggcgcc gatccaaccg gcggcgcttg cgaccaagaa agcagatttc
     1981 atcatcatgt ttcgcgcttc caatgatgag gtggcggagt tggcagcatc gaaagttggc
     2041 agcaaattga atcgtgtttt catgaaagat acaggtctcg acatttacgg tagcggtttg
     2101 gtggccaaag aagaggatat caaaaagcgg ccggatttcg tcagggccta tgttgaaggt
     2161 accatggaag gattgcgcta cacccgtgat aatcaggagg aggcgttgca gatcctgctt
     2221 aaacaaaaac ccgagttaga taaagctctc gccaccctgc agctgaggaa cgctctttat
     2281 gaggtcatgt tgccgccaga atcggtgcaa tccggcctgg ggtacatgaa accggatatc
     2341 atggaaaaaa cggtgcgcgt cacaaacgaa tatttcgatg tggcgcgcaa ggttacctca
     2401 aaggaagttt ttaccaacca atttatcaaa agataagaat cctagttcgg gtccgaagtg
     2461 gcggtccctg atcgctgctg aaggaagttt gtactgagca gtttaccaag agatcattgc
     2521 cttgctcatt ctgggtccga atgctgtccc taatcgctgt tgaaggaatt ttgtaccgag
     2581 cagtttacca agagatcatt gcaatgctca ctctgggtcg gaatgccgtc attaatcgct
     2641 gttgacaatg tctgccgggt gtttaccagc ggcgcccgaa tggtcaatgc tttagatcgc
     2701 atttctttcg agattcacac cggcgaattt gtctccattg ttggacccag cggctgcggc
     2761 aagagcacgc tactcaaaat cctttcggga cttctccccg cgtcatccgg cacgatagtg
     2821 atgagcggca aagccgttga cgaacctgtg gaaaacgtcg gcatggtgtt tcagtctccg
     2881 gtgctgttga agtggcgtcc ggtattggg
//
LOCUS       DASUTG010000763         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1301425, whole genome shotgun sequence.
ACCESSION   DASUTG010000763 DASUTG010000000
VERSION     DASUTG010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301425"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VF089_17705"
     CDS             complement(<1..307)
                     /locus_tag="VF089_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6175850.1"
                     /translation="MKDREIHAAVRILRREAPKWQTPVVTLIAEASDSPFKVLISCIL
                     SLRTQDSTTAQASRRLFALADSPEAMVQLSPKKIEQAIYPVGFYRIKSKTILEICRNL
                     "
     gene            389..961
                     /locus_tag="VF089_17710"
     CDS             389..961
                     /locus_tag="VF089_17710"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX6175851.1"
                     /translation="MVVQKISKALKARSPKTITGNHYKPAAVLIPIQEREDEDYLVLT
                     KRAERMSHHSGQVAFPGGRVDPGDRSEADAALRESYEEIGIHPSEVRILGQLDQAEAG
                     YGFLVTPFVGVIPASCDFVLNPSETAAVCSVPISALLSPPNFAIDQYLSRRGNPSYHF
                     HVNGWDVWGVTARIIVQFLELVYDFEVKKL"
     gene            complement(1087..>2004)
                     /locus_tag="VF089_17715"
     CDS             complement(1087..>2004)
                     /locus_tag="VF089_17715"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175852.1"
                     /translation="MAAWPCAAATKIRMAQPVIAMMYAPLYFGVDQGTFADEGFELEF
                     MAMRTDLAIAALGTGDVDYILHGGAALRAGAQGFPLKLIFALDHKTPFWLVARPQIKK
                     VRQLTGKKIGISFPGDTPQIILKRFLRRQGIDPDRDVAYVSGQFSPTAMQSLLGGVLD
                     AAVLAPPFNVIGAEQGLTMLTFLGESVPDATSSNGIVTSAHKIKSSPDQVRRIVRASL
                     KSLSGFRRNKASAIQFLAAHFEIPKNIAIKAYSNALEILTPDGEISPEKVRQILSMMQ
                     DIGKKEVTAVSPASLIDFSFLREAQKELK"
BASE COUNT          449 a          524 c          548 g          483 t
ORIGIN      
        1 tcaagttccg gcagatctcc aagatggttt tcgatttgat gcggtaaaat cctaccgggt
       61 agatcgcttg ctcgatcttc ttgggactca attgcaccat ggcttcaggc gagtctgcca
      121 aggcaaacag ccgccgtgac gcctgcgcgg tggtgctgtc ctgggtgcgc aggctcagaa
      181 tgcaagagat gaggaccttg aaaggactat cggatgcctc ggcgatgaga gtaaccaccg
      241 gcgtttgcca cttcggcgct tcccggcgca gaatccgcac cgccgcgtga atctcacgat
      301 ctttcactga gtagtttttg tcttgccgat tggctaaatt gaaccagctt catatcacag
      361 atcgctgtcg gatcaacatt tctcagctat ggttgttcag aaaatatcta aagctctcaa
      421 agctcgttcg ccaaaaacca ttacaggaaa tcattacaaa ccagcggcgg tgttgatccc
      481 catccaggaa cgggaagacg aggactatct tgtcttaacc aagcgcgccg agcgtatgag
      541 ccatcacagc ggtcaagtgg catttcccgg cgggcgtgtg gatcctggcg atcgcagcga
      601 agcggacgcg gcgttacgcg agagctatga ggagataggt attcatccta gtgaagtgcg
      661 gatcctgggg caattggatc aagcagaggc aggctacggg tttcttgtta ctccttttgt
      721 cggcgtgatc cctgcgtctt gcgacttcgt attgaatccg tcggaaaccg cggcggtgtg
      781 ttcggtaccg atctcggcgc tattatcgcc gcccaacttc gctatagacc aatatctttc
      841 ccggcggggc aatccgagct atcactttca tgtgaacggt tgggatgtgt ggggcgtcac
      901 cgccagaata atcgtgcagt tcctggagct tgtttatgac ttcgaagtta agaaactttg
      961 accctgcttc tgccagcaat gttttctaag gtcatgatct gccgctgttt gttatccgta
     1021 tgtatttagt caccgcttca ggcgtgggta gtctgcatgt gatcaccgac gattgtccct
     1081 cgcctgctat ttgagttcct tctgtgcttc gcggagaaaa gagaaatcga tcagcgaagc
     1141 cggactcaca gcggtcacct ctttttttcc gatatcctgc atcatagata aaatctggcg
     1201 gactttttcg gggctgatct caccgtctgg agtcaaaatc tccagtgcgt ttgaataggc
     1261 tttgatcgca atgttcttcg gaatttcgaa atgggccgca agaaattgaa tagctgatgc
     1321 tttgttgcga cgaaaaccag agagcgattt aaggctcgcg cgtacgatgc gcctcacctg
     1381 gtccgggctg gacttaatct tatgcgcgct ggtgacgatg ccgttggaag aggtggcgtc
     1441 aggtacggat tcgccaagaa aggtgagcat ggtcaagccc tgctcggcgc cgattacgtt
     1501 gaacggcggc gccagtaccg cggcgtctag gacgccgccc aagagactct gcatagccgt
     1561 gggcgagaat tgacctgaga cgtaagcaac atcacgatcc ggatcgatcc cttgccgacg
     1621 cagaaaacgt ttgagaataa tttgcggcgt atcccctgga aagctgatgc cgatcttttt
     1681 tccggttagc tgtcggacct tctttatctg cggccgcgcg acaagccaaa acggtgtctt
     1741 atgatcgagg gcaaaaataa gcttgagcgg gaaaccctga gcgccggcgc gcaacgcagc
     1801 accgccgtgc aggatgtaat cgacatcacc ggtacctagc gcggcaatcg ctagatccgt
     1861 gcgcatggcc ataaattcca actcaaatcc ctcatcggca aaggttcctt gatcgacgcc
     1921 gaaataaagc ggcgcataca tcattgcgat cactggctga gccatgcgaa tcttagtcgc
     1981 cgcggcgcac ggccatgccg ccat
//
LOCUS       DASUTG010000764         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_117908, whole genome shotgun sequence.
ACCESSION   DASUTG010000764 DASUTG010000000
VERSION     DASUTG010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117908"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VF089_17720"
     CDS             <1..1701
                     /locus_tag="VF089_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEX6175853.1"
                     /translation="DALWPYRDDNGAHFIAPAEGWDTDAIARPLGVYYRVDRQSVVDI
                     QAAIYDTGAVYVSANVHEGWDVKPKKGAVTHKTLPVVNASNKGLGGHAFALVGYNDVG
                     FIVQNSWGGRWGASGFAVLPYDDWIANGTDAWVMSLGAPVQNLRQRQYFVRTSAGLSS
                     TPTSSGLIGPALARRQPSEMCWSEQEAYWHTIVTGNDGLVVNRLPHVADERGNVDWVS
                     YQQPLKWFKNNARNAPWRIAIYAHGGLNSEGESIERIRLLGPCFRENGIFPVFTTWKS
                     GWHETLASMLTDGMKNLFGDQIAPRRGLGDMLVEASDRALEVFLRGVIGKSIWSEMKE
                     NVTRSAEAGRGIDQFAEQLKRLAADSGGKLQVHLVGHSAGSFICGRLLSELRQNNIKV
                     KSCTLYAPACDLKFALDYFKPAIEGHQLARSEFRIHVLSDRLERDDSVGPYQKSLLYL
                     VSRALDRWHKTPLLGLANAFDGARATEEYWHSDTLDQVKQWQKFFWGSSAPKGFASEG
                     NPKLSGNLQVLSQAQVNVGTRYIKSSHGCFDNDLDVIRPTLKNIFGSNLRRDVIDLDY
                     "
     gene            complement(1885..2364)
                     /locus_tag="VF089_17725"
     CDS             complement(1885..2364)
                     /locus_tag="VF089_17725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6175854.1"
                     /translation="MKSRITVKKISTEKELEQAMRIRIRVFVREQGVPAEIELDDEDK
                     RALHLLASFGGRAVGTARIVMRGGQAKIGRMAVLKSYRRKKIGRNLLTRAVMTAKKQG
                     ARKIYLHAQVAVLRFYKSAGFRCVGPVFDEAGIAHRKMVLARTTPRWTETQRRNRRT"
     gene            2532..3653
                     /locus_tag="VF089_17730"
     CDS             2532..3653
                     /locus_tag="VF089_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175855.1"
                     /translation="MGSSLVAAESDRSLLLNGVCYVLFAYDAARSIDLDAAERRIHEA
                     TQRQTIQHKRRAPSYLEYQPPPLRLSQEIQPIKIGGYSSHRMVDLVLYDFGAVSVIYS
                     IAIQGPLAALLALSEDLYDNEILLTESRVRGEQIISAVGDAAINVHTASVVEDYVIFR
                     IAELDPPTSPEAFCASHARDIARILRAERQSLSTQETDDALSARTSFGTDDLTIIDWN
                     AALLVDREGDDVQAVLEFANVELLEMRYLDQKLDRALSEAYETLSKRSWSLPRFLGYY
                     STDLRRVAELQVDNALLFEGVNNTLKLLGDQYLARVYRLVNRRFHLAEWDSSIIRKLQ
                     TLESIYEKIADQAATRRLEILEWVIILLIAFSILLEFIH"
     gene            4753..5241
                     /locus_tag="VF089_17735"
     CDS             4753..5241
                     /locus_tag="VF089_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175856.1"
                     /translation="MLTRRKIIFSLVLWGALTAVAGWASALESPGKYSKLSNKELKSR
                     ALTVVKAVRKLVYSYNEKDRDLTNDFDADYLATRTTERQSLRDQWRKKVDDAQASSVR
                     DYRQNFASDAALLREELQRRLSKSRQRRDMPKLYSSPANVLALEAVADDLELLAKSLP
                     ET"
BASE COUNT         1198 a         1471 c         1406 g         1183 t
ORIGIN      
        1 gacgctctct ggccctacag agatgacaat ggggctcact tcatcgcgcc agccgaggga
       61 tgggacaccg atgcgattgc ccggccacta ggggtctact atcgagtcga ccgccaatcg
      121 gtggtcgata ttcaagccgc gatttacgac accggcgcgg tctacgtgtc cgccaacgtg
      181 catgaaggtt gggacgtcaa accgaaaaaa ggcgccgtaa ctcacaaaac gctgccggtc
      241 gtcaatgcca gcaacaaagg attaggcggc cacgctttcg cgctcgtcgg ttacaacgac
      301 gtcggtttta tcgtgcagaa ctcctggggc gggcgatggg gtgcttccgg gttcgcagtt
      361 cttccttatg atgactggat tgccaacggc accgacgcct gggtgatgtc gctcggcgcg
      421 ccggttcaga atctgcggca gcgacaatac ttcgtcagga ccagtgcggg gctttcatca
      481 acaccgacga gttccggatt gatcggcccg gctcttgccc ggcgccaacc gtcggagatg
      541 tgctggtctg agcaggaggc ctactggcac accatcgtca cgggaaatga cggactcgtc
      601 gtcaatcgtc tgccccacgt cgccgatgaa agggggaatg tcgactgggt gagctatcag
      661 cagcctttga aatggttcaa aaacaacgcg cgtaacgccc cctggcgcat cgccatttac
      721 gctcatggcg gactcaactc tgaaggggaa tctatcgaga ggattcgact ccttggaccc
      781 tgcttccgcg aaaacggcat ctttcccgtg ttcacgacct ggaagagcgg ctggcatgag
      841 acgcttgcga gtatgctgac cgacggtatg aagaacttat ttggcgatca gatcgcgccg
      901 cggcgcggat tgggcgacat gctggtggaa gcctccgacc gcgccttgga agtattttta
      961 cgtggcgtga tcggtaaaag catctggtcc gaaatgaaag agaatgtgac ccgctccgcc
     1021 gaggcggggc gtggtatcga tcaattcgcg gaacagttga agcgcttggc cgcagattcc
     1081 ggcggcaagc tgcaggttca tctcgtcggc cactcggcgg ggtcgtttat ctgcggccgt
     1141 ttattatccg agctccgaca aaacaatatc aaagtaaaat cctgcaccct ttatgctcca
     1201 gcatgcgatc tcaagttcgc gctggattac ttcaaaccgg caattgaagg ccatcaactc
     1261 gcgcgatccg agtttcgcat ccacgtttta tcagatcggc tcgagcgtga tgacagcgtg
     1321 gggccctacc agaaatcctt gctgtatctg gtaagtcgcg cgcttgatcg ctggcataaa
     1381 actcctttgc tcggcttggc caacgcattc gacggcgccc gggccacgga agaatactgg
     1441 cacagcgaca ccctggacca ggtcaaacaa tggcaaaagt tcttctgggg cagttcggca
     1501 ccgaaaggct ttgcgtcaga gggcaaccct aagctaagcg gcaatcttca ggttctgtca
     1561 caagcacagg tcaacgtcgg cacgcgttac attaagagca gccacggctg cttcgataac
     1621 gatctcgacg tgattagacc gaccttaaaa aacatcttcg gcagcaatct gcggcgcgac
     1681 gtcatcgatc tagattacta gatggaattt ttccagaacg ataacttcat ccgaccataa
     1741 agctcgtcgc caggccctgt tgcttgagat cgtgcacaat atgcggtcag gagatgggcc
     1801 gtcgcagtag cgccgctctg aatctctcgc gcaagttagc tgatgtcgct aggatgagat
     1861 cgatcgccgc ccagcttggc acgatcacgt cctccgattt ctcctctgag tttcagtcca
     1921 tctaggcgtc gttcgcgcca aaaccatctt tcgatgagca atgccggctt catcgaatac
     1981 cggaccgacg cagcggaacc ccgccgattt gtagaatcgg aggaccgcga cttgggcgtg
     2041 gagataaatt ttccgagcgc cctgcttctt tgctgtcatg accgcgcggg tcaataagtt
     2101 cctgccaatc ttttttcgcc gataactttt gagaacggcc atgcggccga ttttggcttg
     2161 gccgccgcgc atcactatcc gagccgtgcc aaccgccctc cctccaaaag aggctagcaa
     2221 gtgaagggct cgtttatcct cgtcatctaa ctcaatttct gcgggcacgc cctgctcccg
     2281 gacaaacacg cggatgcgaa tgcgcatggc ctgctccaac tccttttcag tggagatttt
     2341 tttcacggtg attcttgatt tcactgtgct gcccacggca tcaacatcga tggcgctgga
     2401 tagccgttcc gagacagcgc caaacagaag aaatcaattt ccacgaagct gtagaatttc
     2461 cccgtcactc ttggccgaac cggtaacctc tgccgatgct atgcgtccgc gcttcgtgtt
     2521 agagtaggcc catgggctct agtctcgtcg cggcagagag cgatcgatca ctgcttctaa
     2581 atggcgtctg ctatgtgctc tttgcctatg atgcggcgcg ctccatcgat ctcgatgccg
     2641 ccgaacgtcg catccacgaa gcgactcagc gccagacgat tcagcacaag cgccgcgctc
     2701 ccagctatct tgaatatcaa ccaccaccgc tgagattaag tcaggagatc cagccgatca
     2761 agatcggcgg atatagcagc catcgaatgg tggacctggt tctctacgat ttcggcgccg
     2821 tctcagtcat ttatagtatt gcgattcagg gacccttggc ggctttgctg gcgctcagcg
     2881 aagaccttta tgacaacgag attctgctga ccgaatcgcg cgtgcgcggg gaacagatca
     2941 tcagtgcggt tggtgatgcg gcgataaacg tgcataccgc aagcgttgtg gaagactacg
     3001 tcatctttcg gatcgctgag ctggacccgc ccacaagtcc ggaggctttt tgcgccagcc
     3061 atgcacgcga tattgcaaga attctgcgcg ccgaacggca gagtctttcg acccaggaaa
     3121 cagacgacgc gctaagcgcc cggacgtcat ttggcaccga cgatttgacc atcatcgact
     3181 ggaatgccgc gctgctcgtt gatcgcgaag gcgacgacgt tcaagcggtt ttggagttcg
     3241 ccaacgttga attgttggaa atgcgctacc tcgatcagaa actcgaccgc gcgttgtccg
     3301 aagcttacga aaccctctcc aaacgctcgt ggagcttgcc gagatttctc ggctactaca
     3361 gcactgattt gcgtcgggtc gccgagctgc aagtggacaa tgcgctcttg ttcgaagggg
     3421 tcaataacac actcaagctt ctcggcgatc agtatctggc ccgcgtctac cgtttggtca
     3481 atcggagatt tcatctcgct gaatgggatt cgagcatcat tcgcaagctg caaactctcg
     3541 aaagcattta cgaaaaaatt gccgatcaag cggccactcg gcggttggaa atccttgagt
     3601 gggtgatcat tctgctgatc gctttttcca ttttgctgga gtttattcac tgacccgatc
     3661 acacaggcga ccgaaaagac gacatcttgc cgctactcaa cttttgaggc gattcggcag
     3721 ctgagcaaag tatgaaatgg gttccgaatt gctcctatat tcctccgctt gggccatgga
     3781 gcacgtgaac catcgagccg cgcaccccta cttctcgggt aggttttgcg ctaggatcaa
     3841 tttacttgag ctagaaaatg cgcgccgtcg atcacaagaa ccaccgcata gccgttcacc
     3901 ccaggtggaa tgccgagacc cgatcttggg cgtgcgacgc cacagtttat gtaacggaag
     3961 ccggtcgctc gcaagccatc ccgttttttg acccggtgcg ccggtataag atcaaagcgt
     4021 ccgcggtaac cgcgatccta aacgctattc gatattggat agatcacggc aaacccgaac
     4081 agttccctca gcaatagcga aatccaccgc acatcgaacg aatgccgctt cacctctttc
     4141 gatctttgat agcttcgctt gaaacccagc cgccaacggc gactttaaag agtcagatga
     4201 tattaaacat ttcccaaaaa acgctaaact aaacatgaag atgggccgcc ggtacatagt
     4261 gggtgggggc tcgctgagct ggatgtactt acgtgagaga ctacgctgac ataggagctg
     4321 gagcagcttt agcgctggcg gtgtttctag cgcttgggct aatccttcat tggttctttt
     4381 ttatctgtgc ccttccggcg tttgggcttt tcgtctttac cgaatgggca accgctcgcc
     4441 aacaggcgcg cagcccaggg taccgctata acttcgacga agacggcaac atcgtggcca
     4501 tccagtgcga aaactgtgga agagcaagtc acaaccaaca tgatatagag tcaaagtttt
     4561 gtagtttctg cggccgttat cacaatcgcc atgacaactt gaaaaaagcc gccgggaact
     4621 cgccatcatg aagcatcgtg gacatttcac cgttccgctc ctaatacgag ctatgctgcg
     4681 gcaattccgg gtgcggctgc tgcgcatctg ctgacccgtt aattgtgcta gactatgact
     4741 tggtaggctc gcatgctaac cagacgtaag atcatattct ctcttgttct ctggggcgcg
     4801 ctgactgccg tagccggttg ggcttcggct ttggagagtc caggcaagta ttccaagctc
     4861 tcgaataagg agcttaagag ccgagctttg accgtagtca aagcagttag gaaactggtt
     4921 tattcctata acgaaaaaga tcgtgacctg actaacgatt ttgacgcaga ttatctggcg
     4981 acgcgcacaa ccgaacgaca atcgctgcga gatcaatggc gcaaaaaggt cgacgacgcc
     5041 caggcatcct cggtgcgcga ttaccggcaa aactttgcgt cggacgccgc actgctgagg
     5101 gaggaactcc agagaagatt gtccaagagc cggcaacgcc gagacatgcc gaagctctat
     5161 tcaagtcccg caaatgtgct agcccttgag gcagttgccg acgacctcga gctactcgct
     5221 aaatcacttc ccgagacgtg atcatcgacc ggaattac
//
LOCUS       DASUTG010000765         5101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1747412, whole genome shotgun sequence.
ACCESSION   DASUTG010000765 DASUTG010000000
VERSION     DASUTG010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747412"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1817
                     /locus_tag="VF089_17740"
     CDS             84..1817
                     /locus_tag="VF089_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6175857.1"
                     /translation="MIQTKGLTGAQSMLRVLARMGVEKIFASPGSEWSPVWEALAEPG
                     VEGMPQYLSTRHEEVAVGMASGYAKATGKLPAVMIHTTVGSLHATMGMRGAVREQVPM
                     VVLSGESIGYGEDEGPDPGGQWLGHLSNIGGPAKLVEQTVKWSFGVNNKAILPSTIQR
                     ACQLAMAAPKGPTFVSLSMEHLFDKMIKDVPSVGFAPGATADPKGIEELAQLLAGAKN
                     PVIVTEDSGRTEKSVHCLVEIAELLGCVVVETRSTGALNFPRTHPLHGGYDPRKALAG
                     ADAIFLLAVIAPWHPASATPSPGATVAILDENPLRHDMPYYGYQADLLLTGEIESSLE
                     QLLAALKSKVSGSDSSRKQRAEEIGKKNNAQRKAWREEALALANQKPMDTRWVAHEVS
                     QVLPADAMVVEETITHRLAIHRYFDALKPGSFFAGCVGGLGTGLSTALGVKSANPKRP
                     VVCVIGDGSFNYDPAPAVYGAAQEHNLPFLTIMFNNQGYLSQKSGIPKYYPDGWAVKS
                     NNFSGLHIAPCPEYAQIIKAFDGYGEKVEDPGEVRKAVERGLRAVAGGQVALLDIRLK
                     PVEQIVERGEG"
     gene            1908..2912
                     /locus_tag="VF089_17745"
     CDS             1908..2912
                     /locus_tag="VF089_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175858.1"
                     /translation="MTHPRIRLLLVLLQLIVVAVSQSAIAAEKLVGIHSARVLSQSMP
                     WIAQEAGLFKKHNLEFPLVFIASSPAVTAAMLGGDAEIGLTGGEGMIRAYIQGATEFV
                     FIGAVKNVLTHSILAKAPIRKPEDLKGKRIGINRIGSNPHYFAVQALKRAGLDGSRDV
                     TMIQSGGAPETLAALLSGGIDAAALTAPMDAQALAHGYDYILYGPDMRIPYAATVFVT
                     RRSVVAKRAPVISRFMRAMAEAAKILHSDREFTYKVLGKQLRLTDRKILDASYNLEIK
                     ALEPRLSIRTEAFQDILDEVAKVDGRAKQVKADDLIDRRFLDDLDKSGFMNRLWQGKS
                     "
     gene            2923..3633
                     /locus_tag="VF089_17750"
     CDS             2923..3633
                     /locus_tag="VF089_17750"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6175859.1"
                     /translation="MNSSFHDQLVATRDRKHSKNHPFFELWAQGKLTKEQTAFYCTQH
                     YHFVGEFLNWLAYQGSQIRERDVKNYLFENLGDEENPDDRHLDMLKDYVAASGVNRDS
                     VESSVVLAATEGLQNWGWRMVYQRPWQGALAGMFIGLESQFLDICKKIVPALHKYYGF
                     APHAREIRFFEEHIYADEIHGSKGFAIVEKYCNTPELQDLAIQMVEEATIRRWRYMND
                     IYWYALHGKQDDTPSLPG"
     gene            3903..>5101
                     /locus_tag="VF089_17755"
     CDS             3903..>5101
                     /locus_tag="VF089_17755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175860.1"
                     /translation="MKVRIDGGVVVGWSGAGHEIIPDGSVVIENDTIRSVGKDKSQAV
                     DQVIDASGKIVSPGFVNLHVHSQLNVGDYLLADVTRKDYLTANWFVFGAPLKEKITPP
                     PAPAVAIGRKYALFSALKNGATTVLDPGGGPGALDEYVAIVGQQGGRVFFCPPYRSSD
                     IFTDTQGRLYYEDREDKGRPGLERAVAFIKQYHGAHNGRVQGILNPAQAETCEPALLK
                     ETAAAARELDVPIHLHAGGNAREFLHILNTYRKPVVEYLAGTGILGPRTTLGHMAITG
                     GHSRIDYPRGDELKLIAESGTTVGHCPHKCAKMAFAMESFDQYLAAGVRIGLGTDTYP
                     LDIIAEMRYASLISRLIDRDASGARPADCFNAATIGGADALGRKDLGRLAPGAKADVV
                     IINLRTTR"
BASE COUNT         1102 a         1428 c         1521 g         1050 t
ORIGIN      
        1 catagcgctt gaacgcagcc gcggtctcga gcatagggtg ctgcggaccg gcggctatct
       61 tttagaaacc gaaaggaaga cacatgattc aaactaaagg gctaaccggg gcgcaatcca
      121 tgctgcgcgt cctcgcaaga atgggcgtgg aaaaaatctt tgcctcgccg gggtccgaat
      181 ggtcgccggt gtgggaggcg ctcgcggaac cgggcgtcga aggcatgccg caatatttga
      241 gcacgcgcca cgaagaagtg gccgtcggca tggcaagcgg atacgccaaa gccaccggca
      301 aactgccggc ggtcatgatc cacacgacgg taggctcgct gcacgcgact atgggcatgc
      361 gcggcgcggt tcgcgaacag gtgccgatgg tagtcctgag cggtgaatcg atcggctatg
      421 gcgaagacga aggacccgat ccgggaggac agtggttggg tcatctctcc aacatcggcg
      481 gcccggcgaa gctggtcgag cagacggtga aatggagttt cggcgtcaac aacaaagcaa
      541 ttttgccttc gacgattcaa cgcgcctgcc agcttgccat ggcggcgccg aaggggccaa
      601 cattcgtttc tttgtcgatg gagcatctgt tcgacaagat gatcaaagac gttccctccg
      661 tcggtttcgc gcctggagcc accgccgatc caaaggggat tgaggagctt gcgcagctgc
      721 tagcaggtgc aaaaaacccg gtgattgtca cggaggactc agggcgcacg gaaaagtctg
      781 tgcattgctt ggtagaaatt gccgagcttc tggggtgtgt ggttgtcgaa acccgcagca
      841 cgggtgcgct caatttcccg cgcactcatc cgctgcacgg cggctacgat ccgcgcaagg
      901 ctttagcggg cgcggacgcg atctttttgc tggcggtgat cgcgccatgg cacccggctt
      961 cggcgacgcc ctcgcccggc gcgacggtgg cgattctgga tgagaatccc ttgcggcacg
     1021 atatgccgta ctacggttat caggccgatc tccttttgac tggggagatc gaaagctcgc
     1081 tcgagcagct cttggccgcg ttgaagagta aggtctccgg cagcgattcg tcgcgcaagc
     1141 agcgcgctga ggagatcggc aagaaaaaca acgcccaaag gaaagcctgg cgcgaagagg
     1201 cgctggcgct cgccaatcag aagccgatgg acacccgctg ggtagcgcat gaagtgagcc
     1261 aggtcttgcc ggccgacgcc atggtcgtgg aagaaacgat cacacatcgt ttggcgattc
     1321 atcgttattt cgatgcgctc aagccaggct cgtttttcgc aggctgcgtc ggcggcttgg
     1381 gcaccggctt gtctacggcg ctcggcgtaa aatccgccaa tccgaagcgg cctgtggtct
     1441 gcgtgatcgg tgacggatcg tttaactacg atccggcgcc ggcggtgtat ggggctgccc
     1501 aggagcataa cctgccgttc ctgacgatca tgttcaacaa ccaggggtat ctgtcgcaga
     1561 aatccggcat cccgaagtat taccctgacg gctgggcggt gaaatcgaat aatttctccg
     1621 gcctgcacat cgctccctgt ccggagtatg cgcagattat caaggctttc gacggctatg
     1681 gcgaaaaggt cgaagatccc ggcgaagtac gcaaagcagt agagcgcggc ctgcgcgccg
     1741 tggccggcgg ccaggtcgcc ttgctcgaca ttcgtcttaa gccggtagag cagatcgtcg
     1801 agcggggcga gggttaacga acaccgtcct gagtgcaagc ggacgacggg ttcacagtcg
     1861 gttacccgtc gtccgcgctt ctacgtgagc ttagattttt cggccgtatg acacacccgc
     1921 gcattcgatt gcttctcgtg ctgctccagc tgatcgttgt tgctgtttct caatcggcta
     1981 tcgccgccga aaagctggtc ggcatccatt ccgcccgtgt cttatctcaa tccatgccat
     2041 ggatcgcgca agaggccggg cttttcaaga aacataacct agaatttccg ctggtcttca
     2101 tcgcctcatc gccggcggtg accgccgcta tgctcggcgg cgatgctgaa ataggcctta
     2161 ctggcggcga agggatgatt cgcgcctaca ttcaaggggc gacggaattt gttttcattg
     2221 gcgcggtcaa gaacgtgctg acccatagta ttttggcgaa agcgccgatt cgaaaaccgg
     2281 aggacctcaa aggtaaaaga atcggcatca atcgtatcgg cagcaacccg cactattttg
     2341 cggttcaagc gttaaaacgc gccgggctcg acgggagcag agatgtaacc atgattcagt
     2401 ccggcggcgc gccggaaact ctcgccgcgc tcctctccgg cggtatcgat gcggccgcgc
     2461 tcaccgcgcc aatggatgcc caggccttgg cccacggtta cgactatatt ctttatgggc
     2521 cggacatgcg catcccttat gccgccaccg tgttcgtcac ccggcgttcg gtggtcgcca
     2581 agcgcgcgcc ggtgatctct cggttcatgc gcgccatggc ggaagcggcg aagattctcc
     2641 atagcgaccg ggagttcact tacaaggtgc tgggcaaaca gttgcgcttg accgaccgga
     2701 aaatactcga cgcttcgtac aacctggaga tcaaagcgtt ggaaccgaga ttatcgatta
     2761 ggacggaggc atttcaggac atcctcgacg aggtggcgaa agttgatgga cgagccaagc
     2821 aagtcaaggc tgacgatttg atcgaccggc gcttcctcga tgatctcgac aagagcggtt
     2881 ttatgaatag actctggcaa gggaaatcct aaggggactg ttatgaacag cagctttcat
     2941 gatcagttag tcgccacccg cgatcgcaag cacagcaaga atcatccgtt ctttgagctc
     3001 tgggcgcagg gaaaactgac caaggagcag accgccttct actgcacgca gcactatcat
     3061 tttgtcggcg agtttctgaa ctggcttgcg tatcagggct cgcaaattcg cgagcgcgat
     3121 gtaaaaaatt atttattcga aaacctcggg gacgaagaga atcccgacga tcgtcatttg
     3181 gacatgctca aggactacgt cgcggctagc ggcgtgaatc gcgacagcgt ggaaagctcg
     3241 gtcgttctcg ccgccaccga agggttgcaa aattggggct ggcgcatggt ctatcaaagg
     3301 ccttggcagg gcgcgctggc cgggatgttt atcggccttg agtcccagtt tttagatatc
     3361 tgcaaaaaga tcgttcccgc tctgcacaaa tattatggtt ttgcgccgca tgcgcgggag
     3421 atccgatttt ttgaagagca tatctacgcc gacgagatcc atgggtccaa aggctttgcc
     3481 attgtcgaga agtattgcaa cacgcctgaa ctccaggatc tggcaatcca gatggtggaa
     3541 gaggcgacca tccgtcggtg gcgctatatg aacgatattt attggtacgc gctgcacggt
     3601 aaacaggacg atacgccgag cctgccgggc tagattagcg ccggcttccg cagcagagat
     3661 ttagcatgtc cggtttaagg ggcgagactg gaggagcgaa ggcacagaag cgaagagttt
     3721 caatgccgtc tccagtccat ggccaaacag ctgacatcct cgacagacgg ctgctccaaa
     3781 cacggcatgt gtacgcatct atgagccaca gacacattgc cttcggtccg gagggctcgc
     3841 cccttgaacc gatgtcactt taactgcggc attcggaatt atccgaatat aaggatcgaa
     3901 caatgaaggt acgcatcgat ggtggcgtcg tcgtgggttg gtccggcgcc gggcatgaaa
     3961 tcatccccga cggttcggtc gtgatagaga acgatacgat tcgctcggtg gggaaggata
     4021 aatcccaggc cgtcgaccag gtgatcgatg cctccggaaa gattgtttcg ccaggattcg
     4081 tcaaccttca cgttcattcg cagttgaacg tcggcgatta tttgctcgcc gatgtgacca
     4141 ggaaggatta tctaaccgcc aattggtttg tcttcggcgc tcctttgaag gaaaaaatta
     4201 ctccgccgcc cgcgccagca gtggcaatcg gccggaagta cgctttattt agcgctttga
     4261 agaatggcgc gaccacggtg ctcgatcccg gcggcggccc cggtgctttg gacgaatacg
     4321 tcgccatcgt cggccaacag ggaggacgag tttttttctg cccgccgtac cgcagcagcg
     4381 atatctttac cgatacgcaa ggccgtcttt actatgagga ccgtgaagat aaggggcgtc
     4441 ccggcctcga aagagccgtt gcttttatca agcaatacca cggcgcccat aacggcagag
     4501 ttcaaggcat actgaatccc gcgcaagctg aaacctgcga gcccgcgctg ctgaaagaaa
     4561 ctgccgccgc cgctagagag ctcgatgtgc cgattcacct tcatgccggc ggcaacgcgc
     4621 gcgagtttct gcacattctg aatacctacc gaaagccggt ggtggaatat ctcgccggaa
     4681 caggaattct cggcccgcga accaccctcg gccacatggc gatcactggc ggccattcgc
     4741 gcatcgatta cccgcgcggt gacgaattga agctgatcgc cgagtccggc acaaccgtcg
     4801 gccattgccc gcacaaatgc gccaagatgg cttttgcgat ggaatccttc gatcagtacc
     4861 tggccgccgg cgtcaggatc ggtcttggca ccgataccta tccgctcgat atcatcgccg
     4921 agatgcgtta cgcctcgttg atttcgcgcc tgatcgatcg cgacgcttcc ggcgcgcgac
     4981 cggccgattg cttcaacgcg gcgacgattg gcggcgccga tgctctgggc cggaaagatc
     5041 tgggccggct tgcccccggc gccaaagccg acgttgtcat tatcaatctg cgcacaaccc
     5101 g
//
LOCUS       DASUTG010000766         4798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_710467, whole genome shotgun sequence.
ACCESSION   DASUTG010000766 DASUTG010000000
VERSION     DASUTG010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710467"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..504)
                     /locus_tag="VF089_17760"
     CDS             complement(61..504)
                     /locus_tag="VF089_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175861.1"
                     /translation="MNNNLFRSARAVLVLVGMLAVPAPQLYAIDSSNRYFAYGLGQKT
                     CEDYVNYREKRLETLEQQHLRYTKDELYEIVDKIVEHWIAGFLTAHNLYVSDTYDVAG
                     KSNMDDLKARLEKSCRANSKQHFVEAMIAVVHQLNPQRVKAGGGK"
     gene            complement(730..1131)
                     /locus_tag="VF089_17765"
     CDS             complement(730..1131)
                     /locus_tag="VF089_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175862.1"
                     /translation="MCGLFKAQRAIPFHSVWGWLQRARASIAVIIVSGWVVAGCATYE
                     PPPPPPPSPFDRAWNAALGAAQDEGVRVTSADRVSGVIRGYRDQQEVTINLQTQADGN
                     VRVELGARGPKGSDPGLAGRISRAYDRRMGR"
     gene            complement(1300..2265)
                     /locus_tag="VF089_17770"
     CDS             complement(1300..2265)
                     /locus_tag="VF089_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX6175863.1"
                     /translation="MKFLKLDANRRKPIRQRIRLALHAAVVLAVVCAATSSVALAQQK
                     SFSSAQEAVEAAVTAARNNNEKEMVAIFGAQAKELLFSADPVADKHRREEFLAAYDER
                     HRLVAEGRDTILLVGKQDWPFPIPLVKRGNAWVFDTAKGKEEILNRRIGENELYTIKT
                     LLAIVDAQREYALKNRDRDGLLEYAQKFVSEPGKKDGLYWEVKPGEPQSPLGPIMAQA
                     RSEGYKRETSSMAHAYHGYYYRILTAQGKDAPGGAYSYLVNGKMIGGFAVVAYPAEYG
                     NSGVMTFVVNHDGNVFQKNLGKNTVATGKSMKEYNVDATWSEVKE"
     gene            complement(2413..3642)
                     /locus_tag="VF089_17775"
     CDS             complement(2413..3642)
                     /locus_tag="VF089_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX6175864.1"
                     /translation="MKTRQVCTRACACVLILLLAVPPLLVAQQQTDAVFRQEELEQIL
                     APIALHPDPLVPQILMASTYPLEVVLADRWTKQNGSLKGDALTKALEAQDWDPSVKSL
                     VNFPQVLAMMSEQLDWTQKVGDAFLQDQKRVLDTIQSLRAKAQASGNLQTTKEQTVIV
                     EEKIIKIEPASPQVVYVPTYNPTVVYGAWPYPAYPPYYYYPPGYVATTAAFSFAAGAA
                     MGAAWGYAWGGANWNGGEVDVDVDRNTTINNNINRDQAKQKLQERGQLNQNGQGKWQH
                     NAEHRKGVSYRDQGTAQKFNRASTSDSIQSREQYRGRAEQGRQDLGGRAAADRASVAD
                     RGGQGSGGGAGDRAGASQRADSGTRGGAFEGVDRGGSSARSASQRGTASRQSFSGGGG
                     RAGGGGARGGGGGGRRR"
     gene            complement(3750..4178)
                     /locus_tag="VF089_17780"
     CDS             complement(3750..4178)
                     /locus_tag="VF089_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175865.1"
                     /translation="MSKSKPLIAIVDDDESVCRAVRRLVRSLSIDAETFTSGQEFLDL
                     LEAMPSFHPDCLILDVQMPGMNGLELKNRLAISGKTIPVIFITAYDEVGVREKAFAAG
                     AVAFLRKPFNDELLIKTMHGALEGHRMGPQGVIGQAGPRK"
     gene            complement(4340..>4798)
                     /locus_tag="VF089_17785"
     CDS             complement(4340..>4798)
                     /locus_tag="VF089_17785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175866.1"
                     /translation="DVMALVHSDAILQNVRISLELEENLPLVRGDRVQLQQVVLNIML
                     NAFDAMKECPASERKVKLQAGAHGEELIKVAVTDYGTGLRGDNLDKIFQPFYTSKRDG
                     LGMGLSICRSIIEAHGGDLWAENNNGTGATFYFIIPVGGGGKQSSVTDNQ"
BASE COUNT          960 a         1419 c         1199 g         1220 t
ORIGIN      
        1 atcagctcca tggaattgat actcatctag taccgatggt ttccgatcga tggcgggcca
       61 ttattttccg ccaccagctt ttacccgctg cgggtttaat tggtgaacca ccgcgatcat
      121 cgcctcgacg aaatgttgct tactgtttgc ccgacaactc ttctccaacc gggcttttaa
      181 atcatccatg ttcgattttc cggcgacatc gtaagtgtcg cttacgtaga gattgtgggc
      241 ggtgagaaaa ccggcgatcc agtgctcgac gatcttgtca acgatttcgt aaagctcatc
      301 tttcgtatag cgcaggtgct gctgttccag cgtctcgagt ctcttctctc tgtagttgac
      361 gtagtcctcg caggttttct gccctaatcc ataggcgaag tagcggttgc tgctgtcgat
      421 cgcataaagc tgcggagccg gaacggcgag catcccgaca agtaccaaga ccgcgcgtgc
      481 cgatctaaag agattgttat tcattcaatt cacctcccgt aaatgctgcg actgagcatc
      541 gtcactatcc ggtggaatac cgattttgct tgagaaggat ttgtagcggg ttgttgaatg
      601 gccgcctatt ggaccttggt ccaattaaac aggggcaggt acttcgaaca atcgtgaagg
      661 ttcgaggccg gaaaaagctc gaaagatgct ccgggggaac tcctagagtc cctccgttaa
      721 gctcgtttgt tatcgaccca tgcgcctgtc gtaggcgcgc gaaattctac cggcgagtcc
      781 cggatcggat ccctttggac cccgtgcgcc cagttcgacc cgcacgttac catccgcctg
      841 agtctgcaag ttgatggtta cctcttgctg gtctcgataa ccgcggatca ctccgcttac
      901 tcggtctgca gaggtcaccc gcacaccttc gtcttgtgct gcgcctagtg ccgcgttcca
      961 ggcacgatca aacgggctag gcggcggagg aggcggcggc tcgtacgtag cgcatcctgc
     1021 gaccacccag ccgctaacaa tgatcaccgc aatcgacgcg cgggctcgct ggagccatcc
     1081 ccagaccgag tgaaatggta tagctcgctg cgctttaaat aaaccgcaca cgagcaacgg
     1141 ttcgcctctg aagctttgct ccgtatatct cttcgatccg aatgatctca ttgacaatct
     1201 gtttgcaacc ctttcgccgc ggttcttccc ggatcatttg caccggtaag actctggggt
     1261 agtaacactg acccacggca cccggctttt cttttgccgt cactccttaa cttcgctcca
     1321 tgttgcatcg acgttgtatt ctttcatgct cttgccggtt gcgacggtat ttttacccag
     1381 gttcttctga aagacattgc catcgtggtt gacgacgaag gtcatcacac cggagttccc
     1441 gtactccgcc ggataggcaa ccacggcaaa gccgccaatc attttgccgt ttacaagata
     1501 gctgtaggcg ccgccgggcg cgtccttccc ctgcgcggtt aagattctgt aataatagcc
     1561 gtgataggca tgcgccatgc ttgaggtctc tcttttataa ccctcgcttc gcgcctgcgc
     1621 catgattggt cctagaggac tctgcggttc ccccggctta acctcccaat acaaaccatc
     1681 ttttttgccg ggctcgctta cgaacttctg cgcgtactcc aaaaggccat cgcggtcgcg
     1741 gtttttcagc gcgtactcgc gctgcgcatc gacaatcgcc agcaatgttt taatcgtgta
     1801 gagctcgttc tcgccgatcc tccggtttag aatctcctct ttgcctttcg cggtatcgaa
     1861 aacccaggca ttacctcttt tcacgagcgg gatcggaaag ggccaatcct gtttccccac
     1921 gagcaaaatg gtgtctcgcc cttcggcgac gagccggtga cgttcgtcat aggcagcaag
     1981 aaactcctcg cgcctgtgtt tgtcggcaac gggatccgcc gaaaataata gttctttcgc
     2041 ttgagcgccg aaaatcgcca ccatctcctt ttcattgtta tttcgcgccg ccgtcaccgc
     2101 cgcttcgaca gcctcttggg ccgaagagaa actcttttgc tgcgccaatg ccacagagga
     2161 cgttgcggcg cacacgactg ccagcaccac tgcagcgtga agtgccagac gaatccgctg
     2221 ccggatcggt tttctcctat ttgcgtccaa cttcaaaaat ttcatctctc ccccatctca
     2281 aaatgagcct gcaagcatcg atgctacgct aaggacgaac tggaacaccg ggtttcaatt
     2341 attcgatcct tcgattggtt catcgttaca gtgttccatt gttccaagcc gaaacccgaa
     2401 atcagaaact atctaccttc ggcgtcctcc gccgcctccg cctctcgcgc cgccgccccc
     2461 agcccttccc ccaccgccgc taaagctttg ccggctggcg gttccgcgct ggctcgcgct
     2521 acgcgccgag ctaccaccgc gatcgacacc ctcgaaagcg cccccgcgag ttcccgaatc
     2581 agcccgttgg cttgctccgg cacgatcgcc ggctcctcca cccgagccct ggccgccacg
     2641 gtcggcgaca ctggcgcgat cggctgcggc tctgcctccg agatcctgtc tgccctgttc
     2701 agctcgtccg cgatattgct ctctggattg gatagagtcc gatgtgctcg cccggttgaa
     2761 tttctgcgcc gtcccttggt cgcggtacga aacgcctttg cggtgttccg cattgtgctg
     2821 ccactttccc tgtccgtttt gattgagctg tcccctttcc tggagcttct gcttggcttg
     2881 gtctcggttg atattgttat taatagtagt gttgcggtcg acgtcgacat cgacctcgcc
     2941 cccgttccag ttggcaccgc cccacgcgta gccccatgcc gcgcccatgg ccgcacccgc
     3001 tgcaaaggaa aatgcagcgg ttgtagctac gtatccgggc gggtagtagt aatacggcgg
     3061 ataagccggg tagggccagg cgccgtacac cactgttgga ttataggtcg gtacgtaaac
     3121 gacttgcgga ctggccggtt cgatcttgat gatcttttcc tcgacgatta cggtttgctc
     3181 tttggtcgtc tggagattgc ccgacgcctg cgctttggcg cgcaggctct ggatcgtatc
     3241 caaaaccctt ttttgatcct gcaaaaaggc atcccccact ttttgcgtcc agtcgagctg
     3301 ttcgctcatc attgcgagca cctgaggaaa gttcaccagc gactttacac tcgggtccca
     3361 gtcttgcgct tcaagtgcct tggtcaacgc gtcgccctta aggctgccat tttgttttgt
     3421 ccagcggtcg gccagcacga cttccagcgg ataggtcgag gccatcaaga tttgcggaac
     3481 cagcggatcc ggatggagcg cgatcggcgc caagatctgc tccaattctt cctgcctgaa
     3541 aacagcgtct gtctgctgct gagcgacaag tagtgggggc accgccagta acaaaatcag
     3601 tacacatgcg cacgcacgcg tacagacttg tcttgttttc atggtcaacc ctctttcttt
     3661 gagcggcctt aacttttcgt gaagcatcgg tagcactcgc tgcttaatgc gtgattagat
     3721 cgggtaaaag cctccggcag cagaattttt tattttctcg ggccagcttg tcctatgacc
     3781 ccttgcggac ccattctatg gccttccaga gctccgtgca tcgtcttgat tagcaattca
     3841 tcgttgaagg gtttgcgcaa aaacgccact gctccggcgg caaacgcctt ctcgcgcacg
     3901 ccgacctcgt cataggccgt gataaagatc accggaatcg ttttgccgct tatagcgagc
     3961 cgatttttta gttcgaggcc attcatgcca ggcatttgca cgtccagaat caaacagtcg
     4021 gggtgaaacg atggcatcgc ttcaagaaga tcgagaaatt cttgccctga ggtgaatgtt
     4081 tcagcgtcta tcgacaacga ccgcaccagc cgtctcaccg cacggcagac ggattcatcg
     4141 tcatcgacga ttgcgataag gggcttggat ttgctcaaag cgattggcct gtccgcggtt
     4201 gtcgcgagca ttaataaaat taaacgttta atcgtgctgt aaaggatttt agcgaaggaa
     4261 gagaaagctt gtatattgga ctttggtcca atgtcgggaa aaggagggaa gggcttgtct
     4321 tattgtgtgt gtctactgtt tactgattat cggtcactga tgactgcttg ccgcctcctc
     4381 ccacgggtat gatgaagtaa aaggttgcac ccgtgccgtt gttgttctcc gcccaaagat
     4441 ctcccccatg ggcttcgata atcgatcgac agatcgaaag ccccataccc agaccatctc
     4501 ttttgctcgt gtaaaatggt tgaaagattt tgtccagatt gtcgcctctt agaccagttc
     4561 cgtaatcagt cacagctacc ttgatcaact cctcgccgtg agctcccgcc tgcaatttaa
     4621 ccttccgctc gctcgccgga cattccttca tggcgtcaaa cgcattcaac ataatattga
     4681 gcacgacctg ctggagctgc actcgatcgc cccgtaccag cggcaggttt tcttccaatt
     4741 caagcgagat tcgaacattt tggagaatcg cgtcgctgtg caccaaggcc atgacatc
//
LOCUS       DASUTG010000767         7999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_710516, whole genome shotgun sequence.
ACCESSION   DASUTG010000767 DASUTG010000000
VERSION     DASUTG010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710516"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF089_17790"
     CDS             <1..253
                     /locus_tag="VF089_17790"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX6175867.1"
                     /translation="ADLAEVAVVACRHPEWGESVKALVIPKKGTIVEPDRIIQYCKDR
                     LAPYKAPKVVEVVSTLPHTALGKIDRGKLELAELETIGR"
     gene            260..1123
                     /locus_tag="VF089_17795"
     CDS             260..1123
                     /locus_tag="VF089_17795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175868.1"
                     /translation="MPGAIDIVINLHTPEVLPLKPKWRKDFIGGKIGADAEILNGLPI
                     EKYIERMDRAGIQMAFIAASRSGSKGHPSNWQLPYEIVADVVSKYPKRFKGLAGIDPT
                     EGMEGVRNLEHAVRELGFVGAHVYPHWFEMAPHHRKYYPFYAKCVELSIPIQMQVGHC
                     LLYSRERPLRSVGRPITLDTIACDFPELKIIGIHTGWPWVEEMISVAWKHPNVYIGSD
                     AYAPKFWKPEFVHFINSWGQDKVLFGTDFPIIDFERATREIDELGLKEDSKQKLLWEN
                     TTHVYGLDDFG"
     gene            1295..1468
                     /locus_tag="VF089_17800"
     CDS             1295..1468
                     /locus_tag="VF089_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175869.1"
                     /translation="MKAPLPRTNSEFGVAELDGKIYVMGGYPADVKPSPPKSKSTIQN
                     RQMADNRAVAPSA"
     gene            1583..2059
                     /locus_tag="VF089_17805"
     CDS             1583..2059
                     /locus_tag="VF089_17805"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX6175870.1"
                     /translation="MGHSRADANYARRRRRGGWRNLCRRGPPPRGHDFAVYDPNADQW
                     TTLANWPTQRNHLAANAHDGKVYVFGGRFEGGFRSKTTDAVEIFDPKTNSWSSKKSML
                     KPRGGVNGIFANGCFHLFGGEANPNHPYGVFPDHDYYNPASDTWHALSRCQLRCMV"
     gene            complement(2309..2665)
                     /locus_tag="VF089_17810"
     CDS             complement(2309..2665)
                     /locus_tag="VF089_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175871.1"
                     /translation="MTRRSTLAKRPQVLGRFMRVMAEAAKILHTDKEFTFKVLGKQLR
                     IDDRKILETGYNAEIKALEPRLTIRPEAFQAVLDDVAQIDPRAKKVKPDDLIDRRFLD
                     EMTKSGFFDQLWGGKS"
     gene            complement(2785..3300)
                     /locus_tag="VF089_17815"
     CDS             complement(2785..3300)
                     /locus_tag="VF089_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175872.1"
                     /translation="MKPITSLLFWLVLFTTPATSLPADRLLGVHSARVMSQSMPWIAE
                     DAGLFRRYELEFPLVYIGSSPLATAAMLGGEGELLMDGGLGPVRAFVQGTTELVCIGG
                     QKNILTQSILAKPPIKRLEDLRGKKVGVTRIGATTHYFATQAFRKIGMEAGRDYTMIQ
                     SAEHQRCSRHW"
     gene            complement(3546..4220)
                     /locus_tag="VF089_17820"
     CDS             complement(3546..4220)
                     /locus_tag="VF089_17820"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX6175873.1"
                     /translation="MSLTYLLLNLSIIAVPAWYTPDKRTAYYRRLPALGFSIAVVSSC
                     YLLWDILVTARGEWSFNSKYLTGLQVLNIPLEEVLFFITVPYSCLFIYEVVLYATKSS
                     KLRLPASVVIAVILTLIAASLAFYPQGYTAKALASCAFFLVLAFLFDRPLIKSKQYWI
                     WLAICYVPFLIINTVLTALPVVEYNRKAIWGLRVGTIPLEDFFYNFAMLSFYLLFYRI
                     GKKFIQ"
     gene            complement(4246..5760)
                     /locus_tag="VF089_17825"
     CDS             complement(4246..5760)
                     /locus_tag="VF089_17825"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6175874.1"
                     /translation="MLKAFLRSKIKDRLSGYSILINQPDSTKPKSIQSTEKVAVIGAG
                     LAGMGAASLLAERGFKVTLFERNHYLGGKVGCWDIPFEDGNRTKVDHGFHAFFRHYYN
                     LRSFMEKIGATEHLKSIDDYLVLAKDRRHFSFKNVETTPVLNILSLGMNKFFRFRDLL
                     FSPASWRMGEFLEYDEAKTFAKYDSGSFAEFAREASLPKSLQLIFNTFARAFFAPADK
                     LSAAELMKSFHFFYLSHNHGLLYDYFDTDYQEALIQPVTRYLNQHDVAIKLSKSVEET
                     RRDNEAFIVESEPFDYLILATDVVGTKSICANSPWIKTSSEKTYSELTSLKASGGYAV
                     YRIWLDKRTGDELPVFIITEKHEVLDSVTFYHQFDRRAAAWAHVSGGGVYELHCYALP
                     SDAFSVEAVKAAFLAELHFYFPELKGAKIIYDHLQLRHDFTAFYTNLNKTRPEFTTAI
                     PNLYLAGDWVKTGIPAMLMEGAYTSGIMCANSILAKHGLQEEPVRSVPLRGIFA"
     gene            complement(5794..6876)
                     /locus_tag="VF089_17830"
     CDS             complement(5794..6876)
                     /locus_tag="VF089_17830"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6175875.1"
                     /translation="MKQEISTFVESHAQFNGPIRRAGINFNCWYMIAKSSEIQQRPVK
                     REIWKQPVVLFRTQQGEVRALEDRCAHRLVKLSYGRVKDDKIECAYHGWQFNGAGRCV
                     HIPHLGSRTTLPNCEVQSFPVVEKHGFVWIFPGKPALSDTTAPMAMNEWDDLNEISSF
                     VTLRTRAHFSYLIENLMDMYHGSLHAQYQVWTAESLKEVVEDESQVSAIYQATTYYQV
                     KDLGSILQLFIPSLRRLHSVPLTVTYEYPNWKSALGEDFKIYCLICPVNERLTDAYLI
                     HYTSLAKFKGLNNAPLAVRRLLKRALNNVAKKLLANLVRQDVIMIEDEQAAFDQDPLR
                     QPFEVNRAIRRVQELVRRQATDQPLN"
     gene            complement(6956..>7999)
                     /locus_tag="VF089_17835"
     CDS             complement(6956..>7999)
                     /locus_tag="VF089_17835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6175876.1"
                     /translation="HGGRWLIAAGGLVSGAGFLLLGQARDFWQFAIVRWLLVSPGDTL
                     MGSMVVNVSISQWFVRMRGRALALAGMGHGLAKVCMPLLAASLILYVGWRGAWVAFGV
                     LTLALVVGPSLLLMRRRPEDMGLLPDGRSIGENDSVPADQSNKLKSRRSPAEEVTWSR
                     GEALRTRAFWLIVTTFGVAHVGVTGLNLHVFSYVSDQGHPAMVAALVMSIIAVMQFST
                     PMVWGVLAERANVARLIMAKFLIQAFGILLALSDPGVVSLYAGFFLYGIGMGGTSILA
                     EMIWANYFGRISLGRIRGMGSLITSAFSAAGPPFFGLLFDATQSYSLSFSIFIAMLFA
                     SAFLSLFLRAPVK"
BASE COUNT         1970 a         2075 c         2180 g         1774 t
ORIGIN      
        1 tgcggatcta gcagaggtgg ccgtagtcgc gtgccggcat cccgaatggg gcgaatccgt
       61 caaagctctg gtaattccca aaaagggaac cattgttgag cccgaccgta tcatccaata
      121 ctgtaaggat cgtctggctc cctataaggc cccgaaagta gtggaagttg tctccactct
      181 gccccacaca gccctgggaa agatcgacag aggcaaactg gagttggcag agttagaaac
      241 catcgggagg tagccgaata tgccgggcgc tatcgacatt gtcataaacc tgcacacgcc
      301 ggaggttttg ccgctgaaac ccaagtggcg aaaagatttt atcggcggaa agatcggtgc
      361 cgatgcagaa atcttaaacg gtctcccgat cgaaaaatac atcgagcgga tggatcgtgc
      421 agggattcag atggctttca tcgccgcgtc tcgatcaggc agtaaaggac atccatccaa
      481 ctggcagttg ccctatgaga tcgtcgcaga tgttgtgagt aaatatccaa agcgtttcaa
      541 ggggctggct ggaatcgatc ctacagaagg aatggagggt gtgcgcaacc tggagcacgc
      601 tgttcgcgag ctcggcttcg tcggcgctca cgtgtatccg cattggttcg agatggctcc
      661 gcatcatcga aagtattatc ctttttatgc aaagtgcgtc gagctaagta ttcccatcca
      721 gatgcaggtt ggccactgcc ttctctattc acgagagagg ccgctcagaa gtgtcggccg
      781 cccaatcact ttggatacga tagcgtgtga ttttcccgaa ctgaagatca tcggaatcca
      841 taccggatgg ccctgggtcg aggaaatgat ctcagtagca tggaagcacc ctaatgttta
      901 catcggctcg gacgcctatg cgcctaaatt ctggaagcct gagttcgttc atttcataaa
      961 tagttggggg caagacaaag ttctattcgg gacggacttt cccatcattg atttcgagag
     1021 ggctactcgc gaaatcgatg aactcgggct gaaggaggat tcgaagcaga agctgctttg
     1081 ggaaaatacc acccatgttt atggcttaga cgactttgga tgagaaatta ttttatcaac
     1141 tcggggtgac gcgaaagtac ttgggttcca aataggagcg tacgtatgaa acgaattgtt
     1201 ctaagcgcaa tccgttttgg gatgcgcggt ttcagctatc tcgtcattcg cggcggaaac
     1261 gcagagtagt agcgggcgcg cgagcggctg gagtgtgaaa gcgcccctgc cgcgcaccaa
     1321 ctccgagttt ggtgtggccg aactcgatgg caagatttac gtcatgggtg gctatccggc
     1381 ggatgtaaaa ccgtcgccgc cgaagtccaa gtctacgatt caaaaccgac aaatggcaga
     1441 taatcgcgcc gttgccccaa gcgcttaatc acatcatgcc cgcggcattg aaatgtaatc
     1501 ggcggacagc gaccgaaagc agcgaaccga acaaagcggg attcgtcaat acggtttagg
     1561 agtacgatcg ggcaaccggc aagtgggcca ctcgcgcgcc gatgccaact atgcgcggcg
     1621 gcggcgtcgc ggtggatgga ggaatctatg tcgccggggg ccgcccccgc gcggacatga
     1681 tttcgccgtt tatgatccaa atgcggacca atggacgacg ctcgcgaatt ggccaacgca
     1741 gcgaaaccat ctcgcagcca acgctcatga cggcaaagtt tatgtcttcg gcgggcgttt
     1801 cgagggcgga tttcgcagta agaccacgga tgcggtagag atcttcgacc cgaagacgaa
     1861 ttcgtggtcg agcaaaaagt cgatgctgaa accgcgcggc ggcgtcaacg ggatcttcgc
     1921 caacggatgc ttccatctgt ttggcggcga ggccaacccg aatcatcctt acggtgtttt
     1981 tccagatcac gactactata atccagctag tgatacctgg catgccctga gccgatgcca
     2041 attgcgctgc atggtgtgac cggaccggca ttcatcgatg gcagtattca tttgcccggc
     2101 gggggcacgg ctcaaggcgg gagtagcggc tcaacaattc accaggttta ccgtccgagc
     2161 atgagttgcg gctgacgaga agccctggca ctcacagaca tcgtcatttg tcagtgaatg
     2221 attctcgctc tcctgcagta ctgaaagggc ggaccttcgc cagcgcggca gccttgccgc
     2281 ttcaatcgta tatgaaacga gggtcaacct aggattttcc tccccagagc tggtcgaaga
     2341 aaccgctctt ggtcatctcg tcaagaaatc gccgatcaat gagatcatcc ggcttgacct
     2401 ttttggcacg tggatcgatc tgcgcgacgt cgtccaggac cgcctgaaaa gcctcaggcc
     2461 tgatcgtcag cctgggctcc aaggctttga tttcggcgtt gtaaccggtt tcgagaattt
     2521 tacggtcgtc aatcctcagc tgtttgccca agactttaaa cgtaaattct ttgtccgtgt
     2581 gcagaatctt tgcggcttcc gccatcacgc gcatgaatcg cccgagtacc tgaggtcgct
     2641 tggcgagcgt agagcgtctc gtcataaatc ccacggcaac ctgaggaatg cgaagatccg
     2701 gtccatagac gatgtactgg tgacctaggg cgacggccta ggcgtccaag ggcgacgata
     2761 gagttcccgc atcaattgcg ccgcttacca gtgccgcgag catctctggt gctccgccga
     2821 ttggatcatc gtgtagtccc gtcctgcttc catgccgatt ttgcggaagg cctgagttgc
     2881 aaaatagtgg gtcgtggctc cgatccgggt aacgccgacc ttctttcccc gcaaatcttc
     2941 gagtctcttg atcggcggct tggccaaaat actctgagtc aaaatgtttt tctgtccgcc
     3001 gatgcagacg agctcggtgg tcccttgaac aaaagcgcgg acggggccca gacctccgtc
     3061 cattagaagt tctccttctc cgccgagcat tgcggctgtt gcgagtggcg aagagccgat
     3121 gtagaccagc ggaaactcaa gctcgtatcg gcggaagagt cccgcgtcct cggcaatcca
     3181 aggcatcgac tgagacatga cacgcgcaga atgaacgccc aagagcctgt cggcgggcaa
     3241 gctcgtagcc ggcgtagtga aaagaaccag ccaaaacaat agcgaggtta tcggcttcat
     3301 ggaaaacctc ctgtcgaggc tttagtgaat ggctttgaaa cgaggtcaat atcagaaatc
     3361 cggggatgga gcgaacgaaa tccggttctg gcaatctctt ctgtccagct gcgccgatca
     3421 atcagttcat acaacagcga ttcaacagag agcacagata caatccttga cgaatcctga
     3481 agagagcaag gaaggcatcc tgcattacaa cgcagaagag ctttgaagct taaggcgtgg
     3541 acagcttatt gaatgaattt cttaccgatc cggtaaaaca acagatagaa ggacagcatc
     3601 gcgaaattgt aaaaaaaatc ttccagcgga atagtcccta cgcgcaatcc ccagatcgcc
     3661 ttgcggttgt actcaaccac gggaagcgcc gtcagcacag tattgatgat cagaaagggc
     3721 acgtaacaga tggccaacca gatccaatac tgtttgcttt tgataagcgg tcgatcgaac
     3781 aggaatgcaa gcacaagaaa gaaagcgcac gatgccagtg ccttggcggt gtatccttgc
     3841 ggataaaaag cgaggctcgc cgcgattagt gtgaggatca ctgcgattac gacactggcc
     3901 ggcaatcgga gtttgctgct tttcgtggca tacagcacaa cctcatagat gaacaagcag
     3961 ctatatggaa ccgtgataaa aaatagaact tcttcgagag gaatgttgag aacctgcaag
     4021 ccggtgagat atttgctgtt aaagctccac tcgccgcgtg ccgtaaccag tatatcccac
     4081 agtaagtagc agctgctcac caccgcgatc gaaaaaccca gtgccggcag gcgccgataa
     4141 taggcggtac gcttgtccgg tgtgtaccaa gccggtacgg cgatgatgga gagatttaac
     4201 aacaagtagg ttagtgacat tcaatttcca acgtaagctt gaagtttaag cgaagattcc
     4261 ccgaagcggc accgagcgta ccggctcctc ctggaggccg tgcttggcta gaatgctgtt
     4321 ggcgcacata atcccggacg tataagctcc ctccatcagc atcgccggaa tgccggtctt
     4381 tacccagtcg cctgcgagat aaaggttcgg tatagccgtt gtaaattcgg gtctggtctt
     4441 gttcaggttg gtatagaacg cggtaaagtc atgtctcagt tgaagatgat cgtagattat
     4501 tttggcacct ttaagctcgg gaaaatagaa atggagctcg gccaaaaaag ccgccttcac
     4561 agcctctacg gaaaatgcgt cggaaggtag cgcatagcag tgcagctcat aaacgccgcc
     4621 gccagaaacg tgcgcccacg cagccgctct tctgtcgaat tgatggtaga aagtaaccga
     4681 gtcgaggacc tcatgctttt cggtaatgat aaataccggc aactcgtcgc cggttctttt
     4741 gtccagccag atccggtaca cggcgtagcc gccggaagct ttcagcgagg tcaattccga
     4801 gtaggtcttt tccgaagacg ttttgatcca gggggagttt gcgcagatgc tttttgttcc
     4861 gaccacatcg gtcgctagaa tcaggtaatc gaacggctcc gattccacga taaacgcctc
     4921 attgtcgcgc cgcgtttctt ccacactttt gcttagtttg atcgcgacat cgtgttggtt
     4981 tagataccgg gttaccggtt ggatcaatgc ctcttgatag tcggtatcga aataatcata
     5041 aagcaggcca tgattatggc tgaggtagaa aaaatggaaa cttttcatca gctcggctgc
     5101 gcttaacttg tccgccgggg caaagaaagc ccgggcgaac gtgttgaaaa tcagttgcag
     5161 agatttcggt aaactcgctt ctcttgcgaa ctctgcaaaa ctgccggagt catatttggc
     5221 aaatgttttg gcttcatcgt attccaggaa ctcgcccatc ctccagctcg cggggctgaa
     5281 caataggtcg cgaaatctga aaaatttatt catgcccaga gataagatgt ttagtaccgg
     5341 cgtggtctcg acgttcttga aactaaaatg tcttcgatct ttggccagaa ccagatagtc
     5401 gtcgatgctt ttcaaatgtt cagttgcgcc gattttttcc atgaacgaac gtaagttgta
     5461 gtagtgccga aaaaaagcgt gaaatccatg gtccaccttg gtcctgttgc cgtcttcgaa
     5521 cggtatatcc cagcagccga ccttgccgcc gagataatga ttgcgctcga acaaggtcac
     5581 cttgaagcct cgctccgcca gcagggacgc cgcgcccatc ccggccagcc cagcacctat
     5641 gacagcaacc ttttcggttg actgaatgga tttgggttta gttgaatcgg gttggttgat
     5701 gagtatgcta tagccgctta atctgtcctt gattttcgac cgcaaaaaag ctttaagcat
     5761 ccgtgtgaaa tcctgaggcg cgtacccgat gtgtcaattg agcggttgat cagttgcctg
     5821 gcggcgaact aactcctgta cccggcggat ggcgcggttg acttcaaacg gctggcgaag
     5881 cgggtcctgg tcgaaggctg cctgttcgtc ctcgatcatg atgacatcct gacgaactaa
     5941 atttgccaac aattttttag cgacgttgtt tagggcgcgc ttcagaaggc gtcgaaccgc
     6001 gagtggcgca ttattcaagc ctttgaactt cgcaagggat gtgtagtgga taaggtaagc
     6061 atcggttaaa cgttcgttca cgggacaaat cagacaatag atcttgaagt cctccccaag
     6121 ggccgatttc cagttgggat attcataggt gaccgttagc ggaaccgaat gaagccttcg
     6181 cagtgaaggg atgaacagct ggagaatgga gccgagatct tttacctgat aataagtcgt
     6241 agcttggtaa attgccgaaa cctggctttc gtcttccacg acttccttga gcgattcggc
     6301 ggtccatacc tggtactgcg cgtgtaagct gccgtggtac atgtccatca aattttcgat
     6361 aaggtaggaa aaatgagctc gggttctcaa cgtcacgaag ctggagattt cgttgaggtc
     6421 atcccattcg ttcattgcca taggtgctgt ggtatcggac aatgccggtt tccccgggaa
     6481 tatccagacg aaaccgtgct tttcgactac gggaaatgat tgaacttcac agtttggcaa
     6541 agtcgttctg gagcccaggt gcggaatatg cacgcagcgg ccggcgccgt tgaattgcca
     6601 gccatgatag gcacactcga tcttatcgtc tttcacccgg ccgtagctta atttcacaag
     6661 ccggtgcgcg cagcggtctt ccaatgcgcg cacttcgccc tgttgagttc ggaagagcac
     6721 aaccggttgt ttccagatct cccgcttcac cggccgttgt tgaatttccg aagacttcgc
     6781 gatcatgtac caacaattga aattgatgcc cgctcgccgg atcgggccgt taaactgtgc
     6841 atgggattca acgaaggtac tgatctcttg cttcacgtgt ccgtggctcc gaaagatgaa
     6901 acataaacag aattgctatc ccgtacaagt aagccagggg gagcgagaag gagttttact
     6961 tcacgggcgc tcgtaggaaa aggcttagga aggcggaggc gaacagcatc gcgataaaga
     7021 tgctgaagga gagggaatag ctttgagtgg catcgaacaa caggccgaag aacggcgggc
     7081 cggcggcgga aaatgcgctg gtgatcaagg atcccatgcc gcggattcgg ccaagcgaaa
     7141 tgcgtccgaa gtaattggcc caaatcatct ccgccaaaat cgaggtcccg cccatgccga
     7201 tgccatagag aaaaaagccg gcgtaaagag aaacaactcc gggatccgaa agcgccagca
     7261 ggattccgaa agcctgaatc aaaaactttg ccattatgag ccgagcgacg ttggcgcgtt
     7321 cggcgagcac tccccagacc atcggtgtgc tgaactgcat caccgcaatg atgctcatca
     7381 ccagcgctgc taccatcgcc gggtggcctt gatcgctgac ataggaaaag acgtgaaggt
     7441 tgaggccggt cacgcccacg tgggcaacgc cgaaggtggt gacaatgagc cagaatgcgc
     7501 gggtgcgtaa tgcttcacca cggctccagg tcacctcctc agccgggctc cgtcgcgatt
     7561 tcaacttgtt tgattgatct gccggaacgc tgtcgttctc tccgatggat ctgccatcgg
     7621 gcaaaagacc catgtcttcc gggcgccgtc gcataagcag taacgaagga ccgaccacca
     7681 atgcgagcgt cagcacgccg aaggcgaccc atgcgccgcg ccagcccacg taaagaatga
     7741 gcgaggctgc caacaacggc atacaaactt tggccaagcc gtgtcccatg ccggcgagcg
     7801 caagagcgcg gccgcgcatg cgcacgaacc attgggaaat tgaaacattc accaccatcg
     7861 agcccatgag cgtgtcgccc ggactgacaa ggagccagcg aacgatggca aactgccaaa
     7921 aatcccgtgc ctgacccagc agtaaaaaac ccgctcccga gaccaatcca cctgcggcta
     7981 tcagccacct tccgccatg
//
LOCUS       DASUTG010000768         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2193999, whole genome shotgun sequence.
ACCESSION   DASUTG010000768 DASUTG010000000
VERSION     DASUTG010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193999"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..955
                     /locus_tag="VF089_17840"
     CDS             104..955
                     /locus_tag="VF089_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEX6175877.1"
                     /translation="MGDQRSQSIPPERIAAQSFAIGLARAFGGALIFSLPILMTMEMW
                     WLGFYIDEFRLALLLLLTIPLLIGLSHYMGFEDTFGFKDDALDAFVALAVGFIAGAVT
                     LSFFSVIGSDISASEFIGKVSIQAVPGSIGAMFAQSELGGKEGKKEEKKRHAGYGGEI
                     FIMAAGALFLAFNVAPTEEMVLIAHQMTVWHSMALVLASLLIMHAFVYALEFQGTASL
                     PPGTPFWSVFLRFTIVGYAVALLTSLYMLWTFGRTEGLAFHQAISVLVVLGFPAAVGA
                     AAARLIL"
     gene            968..1441
                     /locus_tag="VF089_17845"
     CDS             968..1441
                     /locus_tag="VF089_17845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02588 family protein"
                     /protein_id="HEX6175878.1"
                     /translation="MAARSDHTAKGRQGSEMPALAQSRSASQKQQEKDAIPIWEWIVA
                     GVGLVLVAGVIGFLIYEAFSGNRLPPDIKLSVGSVVQTRNGFLVTVTAINQGGITAEG
                     VDIEGELKRGTETIERSQTTIDYLPPRSNKKAGLFFTRDPRQFELQVRPLGYEEP"
BASE COUNT          413 a          518 c          590 g          509 t
ORIGIN      
        1 gaactcattc cataattctg cggaacagaa attggccagc ggcatagcca ttgctctatc
       61 tgcggaggaa ctctgcgcgc caacaaacaa caggagacat gccgtgggtg atcagcgcag
      121 ccaatcaata ccgcccgaga gaattgccgc ccagtcgttc gccatcggac tagcccgagc
      181 ctttggcggc gcgctaattt tttccttacc gattctgatg acgatggaga tgtggtggct
      241 cggattttac atcgatgagt tcaggctcgc cttgttgctg ttgttgacca ttcctctgtt
      301 aattggcctc tcgcactaca tgggtttcga ggacaccttc ggctttaaag atgacgcgct
      361 cgatgccttt gtcgctttag ctgtgggttt cattgccggg gcggtgacgc tcagtttttt
      421 ctcggtcatc ggatctgaca tctcggcttc cgagtttatt ggaaaagttt ccatccaagc
      481 ggtgcccggc agcatcgggg cgatgtttgc acaaagcgag ctgggcggca aagaaggaaa
      541 gaaggaagag aaaaagcgcc acgcgggcta tggcggtgaa atattcatca tggccgccgg
      601 cgctctattt cttgctttca atgttgcccc gaccgaagaa atggttttga tcgcgcatca
      661 aatgaccgtc tggcactcga tggcattagt cttggcctcg ctcctgatca tgcatgcgtt
      721 tgtttatgcg ctggagtttc agggaacagc gtcgctaccg cctggaacac ccttctggag
      781 cgtttttctt cgctttacca tcgtcgggta tgccgtcgcc ctgctgacga gcctctacat
      841 gctttggacc ttcgggcgca cggaaggttt ggcttttcac caagccatat cggttttggt
      901 cgtgctcggg tttccggctg ctgtgggcgc cgcggcggca aggctgatac tttaggccga
      961 gatagcaatg gcagcgcgaa gcgatcacac agccaagggg agacaaggtt ccgagatgcc
     1021 ggcgctggcg cagtcgcgat ccgcatctca gaagcagcaa gagaaggacg cgattccgat
     1081 ttgggaatgg attgtcgccg gagtcggttt ggtgctggtg gccggcgtga tcggcttttt
     1141 gatctatgaa gctttcagcg gcaatcggtt gccgcctgac atcaaattgt cagtgggttc
     1201 ggtggtgcaa acgcgtaacg gctttttagt cacggttacc gctatcaatc agggcggtat
     1261 caccgcggag ggagttgata tcgaaggaga gcttaagaga ggcacagaga caatcgagcg
     1321 aagccagacg acaatcgatt atttgccgcc gcggtctaat aagaaggcgg gtctattttt
     1381 tactcgagat cctcggcagt ttgaacttca ggtgcgcccc ttgggatacg aagaaccatg
     1441 agagacctgt gtgcagggag ctttcaagcc ggtcttcgta ccagtagcct tgcagggcgt
     1501 agcgctcatg aagccgtgct tcgtaatcac gatcgatggg ggctcgaccc gtcgtattcg
     1561 ggcgcgttct tgatcgctcc gcgtgttagg ccgacataca ccgtcgagtc ggcccaactc
     1621 acctttttaa tccattgagg tgagagcaaa acttttttgc cgggccacca gttccggata
     1681 tcgacaacga tgtaccttat ggaccagctc tcgtcatcga taatgaaatc ctcgacgtga
     1741 ccgatctcgc catcgctggc gccgataata gccggttagc gcttgcgtgc tgcgaagatg
     1801 cggatcttca gcttgctctt tctgcttggc cgttcttacc agaggctaac gtttcagttt
     1861 cgcttggcgc cggcaggttg gaagggtaaa tcatcggccc cataatcggc cccatatatg
     1921 aggcccggtc caatattacg ggtagccata gtaatcgaag tatgcggttt cgtgctgggg
     1981 tgaaaccgct ggcgggtggt ttgcggggct attctcgatc tgattcctgg
//
LOCUS       DASUTG010000769         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1451912, whole genome shotgun sequence.
ACCESSION   DASUTG010000769 DASUTG010000000
VERSION     DASUTG010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451912"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF089_17850"
     CDS             complement(<1..443)
                     /locus_tag="VF089_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175879.1"
                     /translation="MAKFIIQPHGRLNDWVAEEKGYFHEEGLNYELNVEGSRKNTPPL
                     AAVYSSGSLADNRFGAFERYEEGHGRKGKDAGDVSCACHWTINQAAKLEEGVMWAKAY
                     SVCEAAIVVPADSGVSQPGDLAGKEIAVGYHSGSHFSALQALEVFL"
     gene            complement(673..2232)
                     /locus_tag="VF089_17855"
     CDS             complement(673..2232)
                     /locus_tag="VF089_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6175880.1"
                     /translation="MYEKHFGFIKLPFSVAADPGNFYLSSTHQEALTSLHYGIQSRKG
                     LIAVIGEPGTGKTSLLRMFVASAPPTIRTAIIYDPRVSWKTMLRLILSKCGINAPRSD
                     SEGMMRRLESHLKRELKQQRSVVLLVDEAQTLGDDLLEQLRLLSNLEADQEKLLQIVL
                     VGQSELEEKLSEARFSALQQRIALRLQLSALKPEEVDGYITFMLQKAGYQGKELFDRS
                     VVERITYYSRGIPRLISSLCDNALLATYRAFEQSVSVERIEKAARDLKIQPQEGSPAL
                     ADPPAADLPSDLARQFYLGDEQSENDQSWQEEFDASLADLDEARTRNHGRSRSQGARL
                     FMFLALVLITAAALIYFRPGGDHAGLDRFAVGQANDDEPRFPERVEEHREPAALMASA
                     FDPLTSFAPTTRETVQGSGRVFVHTPEHADRPVITQIAKTLQAAGYELPAPRIANGNT
                     RGDVRFFFPGDRREANTVKFLIEAELRKLGFPISLQLLERDGRKFQHAAPGKIEVWLP
                     PLAEQSTTNAS"
     gene            complement(2535..2906)
                     /locus_tag="VF089_17860"
     CDS             complement(2535..2906)
                     /locus_tag="VF089_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175881.1"
                     /translation="MNSNKELKIKQIISAQRGWKAVHCSQSENGQLAMFERAIVCWAL
                     VEQHGGSDGGRTEVRGMEQRFDDLIVVGNVIKAETIGADEVDCNEYFLGYDDPDAHRE
                     SQYWIGEAYRRLSKEKEQRAK"
     gene            2994..3419
                     /locus_tag="VF089_17865"
     CDS             2994..3419
                     /locus_tag="VF089_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175882.1"
                     /translation="MRPTEEFYCGGQKRRAALKKLKLQHSPQPAIDMGAGELTHILFS
                     LQMGCETALADEFHFMPIFEIDMTAPCPCVCVRILLYYADEWLIAMGILNSIPLPVFW
                     GGLHSATVVVVGGYSKRNAGLYIKFGAIHIELSCLYELL"
BASE COUNT          786 a          922 c          883 g          850 t
ORIGIN      
        1 agaaaaacct ctagggcttg cagagccgag aaatggcttc ccgaatggta ccccaccgca
       61 atctctttgc ctgccagatc tcccggctgg gatactccgg aatctgccgg cacaacaatg
      121 gccgcctcac aaacgctgta agccttcgcc cacatcacgc cttcttcaag cttcgcagcc
      181 tggttgatgg tccaatgaca ggcgcagctg acatcgccag catcttttcc cttgcgcccg
      241 tgaccttctt cataacgctc gaatgcacca aaacgattgt cggcaagcga tccgcttgaa
      301 tacacagcgg cgagcggggg tgtgttcttg cggcttcctt cgacattgag ttcataatta
      361 aggccttctt catggaaata gcccttctcc tcggccaccc aatcgttaag cctgccatgc
      421 ggctgaatga taaatttcgc cataagggat tctcctgtct gtcagatgct acttcagcac
      481 tcgacaggtt tgcaaatacc tgacggtaat tcccagaaca ggatgtgaca agaggaagcg
      541 agtgcttgaa agatcaaact cgcgaagcag ctatcgagtg acttggtggc gggtgcgcga
      601 ccggcccgag gccgatgctg gatgcgcttg cgtggagatt gacagcaaac ctgctgcttt
      661 gatcgcagca gtctaactgg cattcgttgt cgattgttct gctaaaggcg ggagccagac
      721 ttcaatcttg cctggcgccg catgttggaa ttttctgcca tcgcgctcaa gcagctgcag
      781 tgagatcggg aagccaagtt tacgaagttc cgcctcaatc agaaacttta ctgtattagc
      841 ttctcttcgg tcgcctggaa aaaagaaccg gacgtctccc cgggtattgc cgttggcaat
      901 cctaggagca ggaagctcat aaccggccgc ttgcagggtt ttagcgattt gggtaatcac
      961 aggccggtca gcatgttcgg gcgtgtggac gaaaactcgg ccacttccct gcactgtttc
     1021 tctcgttgta ggcgcaaaac ttgtaagagg atcgaaagcg ctcgccatca atgccgccgg
     1081 ctcccgatgc tcttcgactc tttccggaaa acgcggctcg tcatcgtttg cctggccaac
     1141 cgcgaatcgg tccaggccgg cgtggtcgcc gccaggccgg aagtaaatta gagcagccgc
     1201 ggtgataaga acaagagcca gaaacataaa caagcgcgca ccctgagaac gagaacgccc
     1261 gtgattccgt gtccgagctt cgtccaaatc ggcgagagag gcatcgaatt cctcttgcca
     1321 tgattgatcg ttctctgatt gctcgtcacc caaatagaat tgtctggcta ggtcggacgg
     1381 caaatcggca gcgggcggat ccgcgagggc cggactgccc tcttggggtt gaattttaag
     1441 atcacgagca gctttttcga tgcgctcaac tgaaacggat tgttcgaaag cgcggtaggt
     1501 agccaacagc gcattatcgc aaagtgaact gattagacgt ggaatccctc tggagtaata
     1561 agtgatccgt tctaccacac tccgatcgaa cagttctttt ccctgatagc cggctttttg
     1621 cagcataaag gttatatagc catctacctc ttccggcttc aaagccgaga gctgaagcct
     1681 aagcgcgatc cgctgctgca gagccgagaa tcttgcttca gaaagctttt cttcgagctc
     1741 ggattgtccg accaggacga tctgcaggag cttttcttga tcagcttcaa gattcgatag
     1801 cagccgcaat tgctcgagca aatcgtcacc tagcgtttga gcctcatcga ctagcaagac
     1861 gacgcttcgt tgctgtttca gctcacgctt taagtgactc tccagccgcc gcatcatgcc
     1921 ttcgctatcg gatcgtggcg cattgatgcc gcatttgctc aggatcagcc gcagcatcgt
     1981 tttccagctt acgcgaggat catagatgat cgctgtccga atagttggcg gcgcactggc
     2041 tacaaacatc ctcagcagcg aggtttttcc agtgccgggt tcgccgatga ctgcaatcag
     2101 cccctttcga gattggatac cgtaatgcag gctggtcagg gcttcctgat gagtcgagct
     2161 caagtaaaaa ttgccgggat cggcggcgac gctgaaggga agttttataa acccaaaatg
     2221 tttctcgtac atagaatgtt tgctttccat ccgagcagtc ggaaagaata cctcctcgcc
     2281 aacaaatctg atcatggcaa aaacagcgaa gcgcgggtgg caataacgta ctgagtgcag
     2341 gaaggttggg atttaagcgc gcgtcgctgt aacaacccgc gttcatcgcg gtaccggtaa
     2401 aatttaccag gaacagtcga tcagtcaaga acaaccagag aacggcgctg ccttactaag
     2461 tttctcgaat agctgacttc gcgcgaggtg aattgattgt tggcgggagc cttgcttttg
     2521 tactgctaaa ccttctactt cgctctttgt tctttttctt tgctcaagcg gcggtaggct
     2581 tcgccgatcc aatactgtga ttctctgtga gcgtcaggat cgtcatagcc aagaaaatat
     2641 tcgttgcaat cgacttcatc tgcgccaatg gtttcagcct tgataacatt gccgacgacg
     2701 atgagatcgt cgaaccgctg ctccataccg cgcacttcgg ttctgcctcc gtcgctgccg
     2761 ccgtgctgtt cgaccaacgc ccagcaaaca atcgcccgct caaacatcgc aagctggccg
     2821 ttttctgatt gagagcaatg aaccgctttc caacctcgct gtgctgagat gatttgtttg
     2881 atcttcaatt ctttgttcga attcataatc ctctcagaag gcctggcgcc gctctgcgac
     2941 cctgagatcc tgcggtaagc cttgagtgat tcagcgaccg ttgatcatgt ctggtgagac
     3001 ccacggagga attctactgc gggggtcaaa agcgaagagc ggcgctcaag aagctaaaac
     3061 tgcagcattc cccgcagcct gctatcgata tgggcgcggg cgaattgacg catatactct
     3121 tcagcctcca gatgggttgt gaaactgcgc tggctgacga atttcatttc atgcccatct
     3181 tcgaaatcga tatgaccgct ccatgtccat gcgtatgtgt tcggatcctt ctttactacg
     3241 cagacgaatg gttgattgcg atgggaatcc ttaattcgat acctctgccg gtcttctggg
     3301 gtggtcttca tagtgcaact gtagtggttg tcggcggcta tagcaaacgt aatgccgggc
     3361 tatacatcaa gtttggagcg atccatattg agctctcgtg cctgtacgaa cttctttaaa
     3421 ggtaaaagga ctgtttcttc c
//
LOCUS       DASUTG010000770         5650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1301962, whole genome shotgun sequence.
ACCESSION   DASUTG010000770 DASUTG010000000
VERSION     DASUTG010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301962"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VF089_17870"
     CDS             <1..1062
                     /locus_tag="VF089_17870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX6175883.1"
                     /translation="WIENDYLDHNLNRVEPNGNIIRMGVEMDPVWRFPVAYWFLNRRP
                     NDEFYTYQQHFQKDIYQRIEARFIRHIFIRYYPEQTRGWPWLFAAVLSLHMMGKYHEA
                     ALVNALIGASKQGFYKKTLPTGFEPEIDPLTGKMFKDDTGALIDQVEPGSWVELPYGV
                     EIQQWNPTYPHEQYGPFNEAALYAVSAAAKMSYMTLTGDVSKANFSSLRHGVNGEREQ
                     WMTLQEFWIRKWKKPGFDEELFRGILSGQIKLPIGKFDKFNRPEFTGRRWGFVTPVQD
                     WQAKMMQVDMCAGSISAIIKEQGADPWETFKEISQEQEWMKELGIARVHSTYQLVNLE
                     TDTAAPAAPPKPEPAGATE"
     gene            1145..3106
                     /locus_tag="VF089_17875"
     CDS             1145..3106
                     /locus_tag="VF089_17875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX6175884.1"
                     /translation="MVALAKSVRCDLPARDRAFSIDRNAIDPEKRTADIIFATETPAK
                     TWDWDLGEYFEILDCGPGADLRRLNNGGAFLCEHDRNIQIGVHLKAETATDSEIGKCC
                     KATVKFGNSAKAKEEFEDVKEGIRSKISVSYQPLEMVLERTSEEGDFYRVTRWEALEN
                     SLVSVPLDDNCQVSRSEAKPKVERHAVTILNPHKLIPMKVRNRSLFYKADDGAPSGGN
                     GGPSQADLDAQKRHIEEAAEKRAQELIKERQKLESDINDIVVAVRKRDKKDFGELAAK
                     YLKTDKPTVDGFRALVLSSDEFKPFEVRFEGEDENSPRIEVVGDRRSGRILTPGEAFI
                     ADERVREWGKKKRSSGGRVQGSMSVNVPNPRVRATATTSQIGMTGVSFEQQTGIIGLP
                     IRRLTVKDLLAQGTTGAQAIKYPQEDTFTNAADVVAEGAAKPEASWDLSNQTATVEKI
                     AVWTKVSDELVADFPAIMSYINERLPLMVELKEEAELLNGTGDLVGILQQSGILAQAK
                     GVLTAADAMYKALTKIRTAGPDGGGFEPDGYVVHPTDWEALRLSKDGNNQYYAGGPFM
                     GPYGNNGIAADSLWGKPVVITAAIAQGTALAGAFKLGAQVFMREGMTIDSTNTDQDDF
                     IKNLMTIRAEIREALAVYRPKAFCKITGL"
     gene            3127..3199
                     /locus_tag="VF089_17880"
     tRNA            3127..3199
                     /locus_tag="VF089_17880"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3160..3162,aa:Met,seq:cat)
     gene            3220..4008
                     /locus_tag="VF089_17885"
     CDS             3220..4008
                     /locus_tag="VF089_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175885.1"
                     /translation="MKTILSLLCVVALAASAFGQSGENLPTFKNVYSTATAPVSGTNE
                     IQTLTFGATITSGTFKLKFDGETTAAITWSATNNTLVANIDAALEALNPIGTGGVTTA
                     AGTIVAGVNGTVTVTFTGNRAKQNVPEITVANESLVGAASTLAVTTATPGVEGDGRIL
                     PKGSIVIALDTGISYQNTGSPPNPTWTKIGDQNTNNTELAAFAAAVPTAAVADLGAVT
                     SAQLTGGEDPTEAEHNALQVDVAANRAKINDLLAKLRTAGILTP"
     gene            4013..4303
                     /locus_tag="VF089_17890"
     CDS             4013..4303
                     /locus_tag="VF089_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175886.1"
                     /translation="MKRQVAKEMVWADADGKLVKDGDESAHELVARKGQLVPERKLER
                     FKGVEAFFTPLSHDIETGEPVLPKQKEAKTKPAAPRKNTARKGKTRVVAKPE"
     gene            4311..4472
                     /locus_tag="VF089_17895"
     CDS             4311..4472
                     /locus_tag="VF089_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175887.1"
                     /translation="MTKAEQEERDRQDAWEKEQAKAGNIIVLPRSGEPGAPDYVPPAD
                     GKKKPAPQQ"
     gene            4485..4808
                     /locus_tag="VF089_17900"
     CDS             4485..4808
                     /locus_tag="VF089_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175888.1"
                     /translation="MALIDESRAAFRELREVMPSTIEFNGITKTGVSQTLTIKRAQEL
                     QNYQIDGSTQFTLEDTDFAALEAEGLKDRVGEVRINGSDPLVITSIDRHPNSAVVHLF
                     LVPSQ"
     gene            4811..5581
                     /locus_tag="VF089_17905"
     CDS             4811..5581
                     /locus_tag="VF089_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175889.1"
                     /translation="MPTIVDFSYFPAFCRQIASQPTVGLKAWDVMLYEVGRVLEQCVR
                     LTTRENVEKIKRSIEFKNRTLPDGKNSSGPPIIYVTKRGLVWFAEEPGPGNAGKAKGP
                     RSIQALYSSGKTFHPMTEFFRYGNPRWARYQAFLAQLKDRQINVREVIGRAGQSWVQI
                     ADALGIRIEAPAYVRNAPAFRGRKHINGTSQKYRTINHVALVMKNVAPILLGTMDGNR
                     ILQTSINGRRKYFETSLRKGVFDDVKSVARQYPGLRAA"
BASE COUNT         1423 a         1605 c         1586 g         1036 t
ORIGIN      
        1 tggatcgaaa acgactacct cgatcacaac ctcaaccgcg tggagccaaa cgggaatatc
       61 attcggatgg gcgtcgagat ggaccccgtg tggcgctttc cggttgccta ctggttcctg
      121 aaccgacggc ctaacgacga attttacacc taccaacagc attttcagaa agacatctac
      181 cagcgcattg aagcccgttt tatccggcac atcttcattc gctattatcc cgagcaaacc
      241 cgcggatggc cgtggctgtt cgcggcggtg ctgagtctcc acatgatggg gaaatatcac
      301 gaggcggcgc tggtaaatgc gcttattggc gcgagcaaac aaggatttta caagaagacc
      361 ttgcctacag gctttgaacc cgagattgac cccctgaccg ggaagatgtt caaggacgac
      421 accggcgccc tcattgatca ggtcgagcca ggctcgtggg tggaattgcc ttacggggtc
      481 gaaattcagc agtggaaccc gacatatccc cacgagcaat atggcccatt caacgaagcg
      541 gcgctctatg ccgtctcggc cgcggcgaaa atgtcttaca tgacgcttac cggagacgtg
      601 tccaaggcga atttctccag cttgagacat ggcgtgaacg gcgagcgcga gcagtggatg
      661 actttgcagg agttctggat ccgaaaatgg aaaaagcccg ggttcgatga ggagctattc
      721 cgcgggattc tctctgggca gatcaaattg ccgatcggga agttcgataa attcaaccgc
      781 cctgagttta ccggccgccg ctggggattt gtcacgcctg ttcaggattg gcaggcgaaa
      841 atgatgcagg tcgacatgtg cgccgggtcg atttccgcta ttatcaaaga gcagggcgcc
      901 gatccgtggg aaacattcaa ggaaatctct caggagcaag agtggatgaa agaacttggg
      961 attgcgcgag tgcactccac ctatcaactg gtcaatctgg agacggatac cgcagcgcca
     1021 gccgcacccc cgaagccgga acctgctggc gccactgaat aactcgcaac tctgcgaaat
     1081 ttctcattcc tgcgaaaatt ttctttgcat tgagacagca accgtgtaga aagccattca
     1141 gaaaatggtt gccctcgcca aatccgttcg gtgcgatctc ccagcgcgag accgcgcctt
     1201 ttccattgat cggaacgcca ttgatcccga aaagcgcacc gcggatataa tttttgccac
     1261 tgaaacgccg gccaaaacgt gggattggga tctcggcgag tatttcgaga ttcttgattg
     1321 cgggcccggc gctgatctcc ggcgtttgaa caacggggga gcatttcttt gcgagcacga
     1381 tcgcaacatt caaatcggcg tccacctgaa agccgagacc gctaccgact ccgaaatcgg
     1441 aaaatgctgc aaagccaccg tgaagtttgg caacagcgcg aaggcgaaag aagaatttga
     1501 ggatgtcaaa gaaggcatcc gctccaaaat ttcagtcagc taccaaccgc ttgagatggt
     1561 tctggaaaga accagcgaag aaggggactt ctatcgcgtg acgcgctggg aagcactgga
     1621 aaacagcctg gtttccgtcc cgttggacga caactgccag gtctcacgaa gcgaggccaa
     1681 accaaaggtc gagcgtcacg cagtcacaat ccttaatccc cacaaactaa ttcccatgaa
     1741 agttcgaaac cgcagtcttt tttacaaagc cgacgatggc gccccgagcg gcggcaacgg
     1801 cggtccctcc caggccgatc ttgacgccca aaagcgtcac atcgaagagg ccgctgaaaa
     1861 gcgtgcccaa gagctcatca aagagcgcca gaaactcgaa agcgacatca acgacatcgt
     1921 tgttgccgtg cgcaagcgcg acaagaaaga tttcggcgaa ctcgccgcaa agtatctgaa
     1981 gaccgacaag cccaccgttg acggcttccg cgcgctggtc ctctcgagcg acgaattcaa
     2041 gccgttcgag gtccgcttcg aaggcgaaga tgaaaactcg ccccgcatcg aagtggtcgg
     2101 ggaccgccgc agcggtcgca tcctcacccc cggcgaagcg ttcattgcgg atgagcgcgt
     2161 gcgcgagtgg ggcaaaaaga agcggtcatc tggcggtcgc gtccaaggct ccatgagcgt
     2221 caacgtcccg aatccgcgag tgcgagctac tgctaccacg tcgcagattg ggatgaccgg
     2281 tgtcagcttc gaacagcaga ccggcatcat tggattgccg attcgccgac tcaccgtgaa
     2341 agacctgctc gcgcagggca ccacgggcgc gcaagccatc aagtatccgc aggaagacac
     2401 cttcacgaac gcagccgatg tggtcgcgga aggtgccgcc aaaccggaag caagctggga
     2461 tctgtccaac cagaccgcca ccgtcgagaa gatcgcagtg tggaccaagg tttccgatga
     2521 actcgtggcc gatttcccgg ccattatgag ctacatcaac gagcgattgc cgctcatggt
     2581 ggaactcaaa gaggaagccg aactactgaa cggcacgggc gatctcgtgg gcattctgca
     2641 acagtcgggc attctggcgc aagccaaggg cgttctcact gctgccgacg cgatgtacaa
     2701 agccctcacc aagatccgca cggctggtcc ggatggcggc ggctttgagc cggacggata
     2761 cgtggttcac cccaccgatt gggaagccct gcggctctca aaggacggca acaatcagta
     2821 ttacgccggt ggccccttca tgggccccta cggaaacaat ggcatcgccg cagactcgct
     2881 ctggggcaag ccggttgtca tcacggcagc gatcgcgcaa ggcacggccc tggccggcgc
     2941 cttcaagctg ggtgctcagg tcttcatgcg cgaaggcatg acgatcgaca gcacgaacac
     3001 cgaccaggac gacttcatta agaacctgat gacgatccgg gcggaaatcc gcgaggcgct
     3061 ggcggtctac cggccgaaag cgttctgcaa gatcaccgga ctgtaagatt gaaacgtaat
     3121 ttgaatagcg gggtggcgca gcggttagcg cgtctgcctc atacgcagaa ggtcgctggt
     3181 tcaaatccag tccccgcaat tcaacgttcc accatcacca tgaaaacaat cctctctctt
     3241 ctttgcgtgg tcgcacttgc ggcctcagcg tttggccaat cgggcgaaaa cctgccgacg
     3301 ttcaagaacg tttattccac cgcaacggcg ccagtcagcg ggacgaatga aattcagacg
     3361 ttgactttcg gcgccaccat cacgagcggc acattcaagc tgaaattcga cggtgagacc
     3421 accgcggcga tcacttggag cgcgacaaac aacacattgg tcgcgaatat cgatgccgca
     3481 ctcgaagcgt tgaatcccat cggcacaggc ggggttacta cggctgcggg caccatcgtg
     3541 gctggcgtta acggaaccgt cacggtcacg ttcaccggca acagagcgaa acagaacgtg
     3601 ccagaaatca cggtcgcgaa cgaatcgcta gttggcgcag cctcaacatt ggccgtgact
     3661 accgctacgc ctggcgtcga aggagatggc cgaatcctgc cgaaaggctc gatcgttatc
     3721 gccttggaca ccggaatcag ttatcaaaac accggaagcc ccccgaatcc tacatggacg
     3781 aagattggcg accaaaacac gaacaacacc gaacttgcgg cctttgcggc ggctgttccc
     3841 accgcggctg ttgctgattt gggtgccgtc accagtgctc agcttaccgg cggcgaagat
     3901 ccgaccgagg ctgagcacaa cgcgctccaa gttgatgtgg cggcgaaccg cgccaagatc
     3961 aacgacctgc tcgcgaaact tcgcaccgcc ggaatcctca ctccgtaacg ctatgaaaag
     4021 acaagttgca aaagaaatgg tctgggctga cgcagacgga aaactcgtca aagacgggga
     4081 cgaatcagcg cacgagctcg ttgcccgaaa agggcagttg gtgcccgaaa gaaaactgga
     4141 gcgattcaaa ggtgtcgaag ccttcttcac gcctctgagt cacgacatcg aaacggggga
     4201 gcctgtgctg ccgaagcaaa aagaagcgaa gacaaaaccc gctgctccgc gcaagaacac
     4261 tgcccgcaaa ggcaaaacgc gcgtcgtcgc gaaacccgag taattgcgac atgacgaagg
     4321 ccgaacaaga agaacgggac cgccaagacg cttgggaaaa agagcaggcg aaagccggca
     4381 atatcatcgt cctgcctcgt tccggagagc ccggggcgcc tgattacgtt cctcccgccg
     4441 acgggaagaa aaagcctgcc ccccagcaat aggggggctg cgtcatggcc ctgattgatg
     4501 aatccagggc agcgttccgc gaactgcggg aagtgatgcc ctccacgatc gagttcaacg
     4561 ggatcaccaa gacgggggtc tcgcaaaccc tgacgatcaa gcgagcacaa gaactccaaa
     4621 actatcagat cgatgggagc acgcagttca cgcttgaaga taccgacttc gccgcgctgg
     4681 aggcggaggg gttaaaagat cgagtcgggg aagtaaggat caacgggagc gatccccttg
     4741 tgattacgag catcgaccgg cacccgaatt ccgcagtagt ccatctcttc ctcgttccga
     4801 gccaataaca atgcccacga ttgtcgattt ctcttatttc ccggcgttct gccggcagat
     4861 cgcgtcacaa cccacagtgg gactcaaggc gtgggacgtg atgctctacg aagttgggcg
     4921 tgtgctcgag cagtgcgtca ggctcacgac acgggagaac gttgagaaaa tcaaacgctc
     4981 catcgagttt aagaatcgaa cacttccaga cggcaagaat agttcgggcc cgccgattat
     5041 ctatgtcacc aaaagggggc ttgtctggtt cgcggaggaa ccgggccctg gaaatgcggg
     5101 caaagcaaaa ggcccgagat caattcaggc cctctattcc agcggcaaaa cattccaccc
     5161 tatgacagaa ttcttccgtt acggaaatcc aagatgggcg cgctatcagg catttctcgc
     5221 ccaactaaaa gatcgacaaa tcaacgtgcg ggaggtgatc ggacgcgctg gccaatcatg
     5281 ggttcaaatc gccgatgccc taggaattcg gatcgaggct ccggcatacg ttcggaacgc
     5341 gcctgctttc cgcggacgga aacacatcaa tgggacttct caaaaatacc gcaccatcaa
     5401 ccatgtggct ttggtgatga agaacgtcgc gccgattttg ctcgggacca tggacggaaa
     5461 ccggatactc caaacatcca tcaacgggcg ccggaaatat ttcgaaacca gtctgcgcaa
     5521 gggcgtgttt gacgatgtga agtcagtcgc gcgtcaatac ccgggattga gggctgccta
     5581 atgggaacaa tcgagcaaat tgtgaatcca ttgccgtttg tgctgtcggc gtggaaaaca
     5641 gtcttggaaa
//
LOCUS       DASUTG010000771         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1898238, whole genome shotgun sequence.
ACCESSION   DASUTG010000771 DASUTG010000000
VERSION     DASUTG010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898238"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF089_17910"
     CDS             <1..699
                     /locus_tag="VF089_17910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6175890.1"
                     /translation="SAALKQTAPAANAGRTSWIAELHSAQKQFCTPKDRPSRRVSMER
                     VMTDLRAALPRDAITTTDAGSFGQWPQRYLEVDHPNSYVSPTLGCMGPGVPSAVAAKL
                     AHPGRLVVAHVGDGGFLMTGQEMATAKQFGAKIITIVYNNDGFNSIRMHQEAQYPGRQ
                     YGTALENPDFAKLGEAYGALGLKVTRDEEFMPALKKAIAVDGSALIEVLTDYEYVTPT
                     ATLSEVAGKAMHGE"
     gene            865..1950
                     /locus_tag="VF089_17915"
     CDS             865..1950
                     /locus_tag="VF089_17915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175891.1"
                     /translation="MNDLRIIDADGHVQERDADWQELLDPPYRKQAPKMVTGPDGKEQ
                     LLLEGKIWAKPSGIGLGIGTAPYSRRPQKTTGMFDPLQRLQDMDLEGIDTAVNFGTTV
                     FLSLPFLEDHDLACAIAHGYNTWLDRYCKTSPQRLKGIALVAMQEPKEAVKELERCIK
                     ELGFVGVATSAHSAGRNLDHPDFYPFYAKAQELGVPVCVHVGSGRPAAAAERFNNAFF
                     VHATTHAFEQMIGVMCIVGGGILELYPKLKVAFLEAGAGWVPYWMERLDEHYEYLQPT
                     VPLMKKPPSEYMRSEQVYYSFEPDEHTLNFVMNYVGEDRLVFASDYNHGDSKFPHTVA
                     SVTERKDLSRVQLGKLMCDNAARLYNI"
     gene            2159..>2876
                     /locus_tag="VF089_17920"
     CDS             2159..>2876
                     /locus_tag="VF089_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175892.1"
                     /translation="MFLMHNLKLVCRLVALLLLWSSTSPAQEKGLRKFHWGVTSLSAS
                     QWIPWIAKEAKLYEKYGMEAELVLLRGSGQSAQAIIGGSILGAPCTLTTVIGANLSGA
                     DLVTVAHTVSGVQSKLVVRPDIKKPDDLRGKRIAVSGFGSLGDFLERYIIKKHGMDPV
                     KDVILLSIGNQPDRLQALYNGVVDAADLSHPADVQAERKGYRVLWDAKKEVSYPSMSI
                     VTRRKHTIDDRDSVMRMVKAH"
BASE COUNT          683 a          822 c          818 g          553 t
ORIGIN      
        1 tcggcggcgt tgaagcaaac cgcaccggca gcgaatgccg gccgcacttc ctggatcgcc
       61 gaactgcaca gcgcgcagaa acagttctgc acacccaaag acagaccaag ccggcgggtt
      121 tcgatggagc gggtcatgac ggatctgcga gctgcgctgc cgcgcgacgc gatcacgaca
      181 accgacgccg gcagcttcgg ccagtggccg cagcgctatc tcgaggttga tcatccgaac
      241 tcttacgtct cgccgaccct gggctgcatg ggccccgggg tgccatcggc ggttgccgcc
      301 aagctcgcgc accccggacg tttagtcgtc gcccatgtcg gcgacggcgg ctttctgatg
      361 accggccagg aaatggcgac ggcgaaacaa ttcggcgcca aaatcattac catcgtctat
      421 aacaacgatg gctttaactc gattcgcatg caccaggaag cgcagtatcc gggcaggcaa
      481 tacggcacgg cattggaaaa tcccgatttt gccaaattag gcgaggccta cggcgcgcta
      541 ggcctgaaag tcacgcgcga cgaagaattc atgccggcgt taaaaaaagc gatcgcggtg
      601 gatggttctg cgttgattga agttctaacg gactatgagt acgtcacccc gaccgcaacg
      661 ttgtctgaag tggccggtaa ggcaatgcac ggagagtagc ggatcacgcg atcatgttct
      721 ttgcataact gccgtctcat caccaagcac acaggccgcg aaggacttga caccgaaccc
      781 taacgggccg ctgaaaaacc ttcttgcagg ctgctcaaaa gagccagagg cagcatgcta
      841 aactgactca cctcaggagg ttagatgaac gaccttcgca tcatcgacgc cgacgggcat
      901 gtgcaggaac gggatgctga ttggcaggaa ctattggacc cgccctaccg gaagcaggcg
      961 cccaaaatgg tcaccggacc tgacggcaag gaacaactgc tcctcgaagg gaaaatctgg
     1021 gcgaagcctt ccggaatagg gctcggcatc ggcacggcgc cttacagccg caggccgcag
     1081 aaaacgaccg ggatgttcga tccgctccag cgtctgcaag atatggatct cgaaggcatc
     1141 gacaccgcgg ttaactttgg cacgacggtt ttcctgagcc tgccctttct cgaagatcac
     1201 gatctggcct gcgccatcgc ccatggctat aacacatggc tcgaccgcta ctgcaaaacc
     1261 agcccgcaac ggttgaaagg gatcgccttg gtggccatgc aggagccgaa ggaagctgtc
     1321 aaggagttgg agcgctgcat taaagaattg ggcttcgtcg gggtcgccac ttcggcgcac
     1381 tcagccggac gcaatttgga tcatcccgac ttctatccgt tttacgccaa ggctcaagaa
     1441 ctcggcgttc cagtatgcgt gcatgtcggt tccggcaggc cggcggccgc cgccgagcgc
     1501 ttcaacaacg ccttcttcgt ccatgcaacg acccacgcct tcgagcagat gatcggcgtc
     1561 atgtgcatcg tcggcggcgg catcctggag ctctatccga aattgaaagt agcctttctc
     1621 gaagccggcg ccggttgggt gccctactgg atggagcgtt tggacgaaca ctacgagtat
     1681 ctccagccga ccgtaccgct gatgaaaaaa ccgccaagcg aatacatgcg cagcgagcag
     1741 gtctattact cattcgagcc ggacgagcac actttgaact tcgtcatgaa ctatgtcggc
     1801 gaagaccgcc tggtctttgc ctccgactac aatcacggcg acagcaaatt tccccacacg
     1861 gtcgcatccg tgaccgagcg caaagacctg agtcgggttc aattaggcaa attgatgtgc
     1921 gacaacgcgg cgcgcctata caacatctaa aagccagtaa gcatgcggca tcagactaac
     1981 atgctgctga aaaaggctct tgcaggctgc tcaaaaagat ccagaggtga ggcgcgcgaa
     2041 aaatcgacga gcggaggcgt acagcggcag tacgttgacg cgaggtgatt gagcagcaac
     2101 gaagcatatg ggtctttttc agcaagcctg ctaggcaata aaaccaatgg aggcgtaaat
     2161 gtttcttatg cacaatttga aactcgtctg ccgactagtt gctttgttgc tgctatggtc
     2221 ttctacttcg ccggcacagg agaaaggcct taggaaattt cactggggcg tgacatcgct
     2281 ttccgcgtcc caatggatcc cgtggatcgc caaggaagcg aagttatacg aaaaatatgg
     2341 aatggaggcc gagctggttt tactgcgcgg ctcgggacag tccgcccagg cgatcatcgg
     2401 cggcagcatt ctcggcgcgc cctgcacgtt gacaacggtc atcggagcca atctgagcgg
     2461 cgcggatctc gtcacggtag ctcacaccgt ctcaggtgtg caaagtaaac tcgtggtccg
     2521 gccggatatc aaaaagcccg acgacctgcg cggcaagaga atcgcggttt caggatttgg
     2581 ctcgctggga gactttctcg agcgttacat catcaagaaa cacggcatgg acccggtcaa
     2641 agacgtgatt cttctctcga tcggcaacca acccgatcgg cttcaagcct tatataacgg
     2701 cgtagtcgat gccgccgacc tttctcatcc ggccgatgtt caagccgagc gaaaaggtta
     2761 tcgggtcttg tgggatgcca aaaaggaagt cagctatccg tcgatgtcga tcgtcacccg
     2821 gcgcaaacat accatcgatg atcgggacag cgtcatgcgc atggtcaagg cgcatg
//
LOCUS       DASUTG010000772         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1008343, whole genome shotgun sequence.
ACCESSION   DASUTG010000772 DASUTG010000000
VERSION     DASUTG010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008343"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..693
                     /locus_tag="VF089_17925"
     CDS             157..693
                     /locus_tag="VF089_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175893.1"
                     /translation="MCYTTFMDLNTEEVKRKVSAWVEQHKQAVLFDPENSTVLDVASG
                     RKTIIPWRDLAAFEEKIHPETQGSYFVLLFEDGKQIALVDPGGVAFAPSFENTGPLPE
                     SPQVVCLKDFHTLKPRIDHYLYDHPDEPPPKETLDLVMLCIAILDGARAVGFDIADLE
                     GELENSLSDIERRTSPTN"
     gene            747..1991
                     /locus_tag="VF089_17930"
     CDS             747..1991
                     /locus_tag="VF089_17930"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6175894.1"
                     /translation="MGIVNNLHCPKCAYQTVTRTEFQCAQCRSILEVEVEISHITRGD
                     FQAMRRSRDPSIWRWFDFFPVEDRSSIVSLGEGGTPLIHARRLGEHANLANLYLKNDT
                     VLPTGSLKDRSNSVGISKAKELGFTTATVMSTGNAAASVAAYCAAARLQSIVMVPAGT
                     APSKIIQARAYGASVVVIDGSFDREVAQLYKAAVKEFGWYDCLSSNPYREGKKSYAYE
                     LVDQFDEQIPDWLIHPTAGGTGIYAAWKGFNELLSLNLIDRAPKLIIAQSAAAAPIVD
                     AFERDLAEIVPMIARDTVAESIQVGNPASLGWRALAAVRESGGRAVAVCDRETLEAQY
                     LIGSQAGIFAEPAAATSVAAAIKLRKSGVIGREDVVVCNLTGHGLKQPESIRISETEL
                     QPIAPTMDALREKLRRGDHEKT"
BASE COUNT          471 a          564 c          585 g          453 t
ORIGIN      
        1 gcgtttacac gatccttttt catctccgtc acttgccgta agcagccgtt ccagctgttc
       61 caacagttcc aagtcgttct aatcgtctga aactgggcga agccctgaaa catttggaac
      121 cattggaaca acaaggggac gagagggaaa tcgtttttgt gctacactac ttttatggac
      181 ctcaataccg aagaggtaaa acgtaaagtg agcgcctggg tcgagcagca caagcaggcg
      241 gtgctgttcg atcctgaaaa ttcgacagtg ttggatgtgg cttccgggcg gaagacgatt
      301 attccgtggc gtgatctggc ggcctttgaa gaaaagattc atcccgagac tcaaggcagt
      361 tatttcgtcc tgctgttcga agacggcaaa cagatcgccc tggtggatcc cggaggtgtc
      421 gcctttgccc catcatttga gaacaccggg ccgttaccgg aatcaccgca ggtagtctgt
      481 ctcaaagact ttcacacgct caaaccgcgc attgatcatt acctctatga tcatcccgac
      541 gagccaccgc ccaaggagac cctcgatctg gtgatgctct gcatcgccat tctcgatggc
      601 gcgcgcgccg tgggattcga tatcgccgac ctggaagggg aactggagaa ctctttgagc
      661 gatatcgagc gcagaaccag tcctacgaat tgaggtcgaa tggagatcgt gcgagatctt
      721 tccctgagtc caggtaaata atttcaatgg gaattgttaa caatctgcat tgtccgaagt
      781 gcgcctatca gacagtgact cgaaccgagt tccaatgcgc gcaatgccgt tccattctcg
      841 aggtcgaagt cgagatcagt catataaccc gtggcgattt tcaggccatg cgccggagtc
      901 gcgacccgag tatctggcgc tggtttgatt tcttcccggt ggaagatcga tcttcgatcg
      961 tttcgttggg cgagggaggc acaccgctaa ttcacgcgcg ccgtctcggc gagcatgcca
     1021 atctggctaa tttgtattta aagaatgaca ccgttcttcc tactggatcg ctgaaagacc
     1081 gcagcaacag cgtcggcatt tccaaagcga aggaactggg ctttaccacg gcgaccgtga
     1141 tgtcgacggg taatgcggct gcttcggtgg ctgcctactg cgccgccgcg aggctgcaaa
     1201 gtatcgtcat ggttccggca ggcaccgcac cgtcaaaaat cattcaggcg cgcgcctatg
     1261 gagcatcggt ggttgtcatc gatggcagtt tcgaccgcga ggtcgcgcag ctttataagg
     1321 cagcggtcaa agagttcggc tggtacgact gcctgtcgtc caatccctac cgcgaaggca
     1381 aaaaatctta tgcctacgaa ctggtcgatc aatttgatga gcagatcccg gactggctga
     1441 ttcatccgac ggccggcggc accggcattt acgccgcctg gaaagggttc aatgaattgc
     1501 tttccttaaa tctgatcgat cgcgctccca agctcataat tgcccagagc gcggcagcgg
     1561 cgccgattgt cgacgcgttc gaaagggatc tggccgagat tgttccgatg atcgcccggg
     1621 acaccgtggc ggaaagcatc caggttggca acccagcctc gttgggctgg cgcgcgctgg
     1681 cggcagtgcg cgaatcagga ggccgcgcag tggctgtctg tgatcgagaa actctcgaag
     1741 ctcaatacct gatcggctcg caggcgggaa tctttgccga gcccgccgcc gcgacatccg
     1801 ttgccgcagc tatcaagcta agaaaatcgg gtgtcatcgg ccgagaggac gttgtcgtct
     1861 gcaatctcac aggtcacggg ctgaaacagc cggaatcgat tcgcatcagc gaaaccgagc
     1921 tccagccgat tgcgccgacc atggacgcgc tgcgcgagaa actccggagg ggcgatcacg
     1981 agaagactta accgctgcgt acgccccaaa caggtttaga tattttgccg aagtcatgtg
     2041 aagaaaagcg gcactcggtg ttacgcctaa gct
//
LOCUS       DASUTG010000773         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_858187, whole genome shotgun sequence.
ACCESSION   DASUTG010000773 DASUTG010000000
VERSION     DASUTG010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858187"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF089_17935"
     CDS             <1..235
                     /locus_tag="VF089_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175895.1"
                     /translation="GTPVTEQLILNLARHPEGCLKGIPAASRKSAMINQADSPEELER
                     AKHLGKALLQLEFDRVVVNSFADSGLSNEVISH"
     gene            254..841
                     /locus_tag="VF089_17940"
     CDS             254..841
                     /locus_tag="VF089_17940"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6175896.1"
                     /translation="MISAVVLAAGRAERMGEQKLLLPLNDKPVLQWVLESALASKVHE
                     VTCVVRDLKAVRQRITLAHDRLHWLINYAADRGQSTSVIAGLWAIEPRSDGALFLVGD
                     QPLVRKDLLDALIQRFEQSSALIVAPSFKGQARNPVLFRRELFPELLRLTGDRGGRFL
                     IEKHKSRADFVEWQQEMPFMDIDVREDYERLKELA"
     gene            complement(851..1948)
                     /locus_tag="VF089_17945"
     CDS             complement(851..1948)
                     /locus_tag="VF089_17945"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX6175897.1"
                     /translation="MLYKSIIRPWLFSKDPEQSHDGTLELLSRLEFLHSPLDHFYRVE
                     DKRLNVQIASLNFSNPVGLAGGFDKNAVALRTISAFGFGFMEIGAVTAQAQPGNPKPR
                     LYRLPEDCALINRLGFNNDGATAIAARLGNLRARGKLPGIPLGINIGRTKIVETKDAV
                     ADFLSTFEKLFSHGDFFTLNVSSPNTPNLRDLQEKRLLADLLSAVQEKNQQLAKSAQR
                     QQKPVFVKIAPDMEFAQVDEIVEVVDSFKLVGIVATNATAFMRDSVKSPLGREPGGLS
                     GRPITTMVTKFISHIYKHTSGRLPIIGVGGIFNAQDAYDKITAGASAVQIYTGFVYEG
                     PAAVKRINKGLIKLLKRDGFKTVGEAVGQAI"
BASE COUNT          538 a          568 c          605 g          545 t
ORIGIN      
        1 aggaactccg gtcaccgaac aactgattct aaacctggcg cggcaccctg aaggttgtct
       61 caaaggcatt ccagcagcga gtcgaaagtc agcgatgatc aatcaggcgg acagtccgga
      121 agaacttgag cgggccaaac acttaggcaa agctctgctc cagttggaat tcgaccgggt
      181 ggtggttaac agttttgctg atagcggact gagcaatgaa gtgatctctc attagcccaa
      241 tacgtattac aacatgattt ccgccgtcgt tctggctgca ggccgtgctg aaagaatggg
      301 tgagcaaaag ctgttgctgc cgctcaacga taagccggtg cttcaatggg tgcttgagag
      361 cgcattagct tcgaaggttc atgaggtgac ttgtgtcgtg cgtgatctca aggcggtgcg
      421 acaaagaatc acgttggcgc atgatcgact gcattggctg atcaactacg ccgcggatcg
      481 agggcagagc acctcggtaa tcgctgggct ctgggcaatc gagccaagga gcgacggcgc
      541 gctatttctg gtcggcgacc agccgctggt caggaaggat ttgcttgacg ctttgattca
      601 acggttcgag cagagctcgg ctttgatcgt tgctccgtcg ttcaagggac aagcgcgcaa
      661 tccggttctg ttccggcgcg aactattccc agagctcctt aggttgacag gcgaccgcgg
      721 cggccggttc ttgattgaaa agcataagag cagagcagat ttcgttgagt ggcaacaaga
      781 aatgcccttt atggatatcg atgtgcggga ggattacgaa cgtttgaagg aactcgcata
      841 gctcaacaag tcaaattgcc tgcccgaccg cttctcccac cgttttgaat ccatctcgtt
      901 tgagcagctt gatcaatcct ttattaattc gcttcaccgc cgccggacct tcatagacaa
      961 atcctgtata gatctgcact gcagatgctc cggcggtgat tttgtcatag gcgtcttggg
     1021 cattgaagat tccgcctacg ccgatgatcg gcagccgtcc actggtatgt ttataaatat
     1081 gagaaataaa ctttgtcacc atcgttgtga tcggcctgcc gcttagacca ccgggttccc
     1141 ggcccaacgg tgatttcacg ctgtcacgca taaatgctgt ggcgttggtg gcgacgatgc
     1201 ccactaattt gaacgagtca acgacctcga caatttcatc cacctgcgca aattccatgt
     1261 cgggcgcgat cttaacaaaa accggttttt gttgccgctg cgccgatttt gcaagttgct
     1321 ggtttttctc ctgaacagcg ctaagtaaat ccgcaagcag ccttttctcc tggaggtcgc
     1381 gcaggttcgg tgtattgggt gaacttacgt tcagggtaaa aaagtcgcca tgcgaaaaca
     1441 acttttcgaa ggtcgagaga aaatccgcta cggcatcctt agtttcaacg atcttggtgc
     1501 ggcctatatt gatacctagc ggaattccgg gcagcttgcc gcgagccctc aagttaccca
     1561 gtcttgcggc aattgccgtt gcgccgtcat tgttgaagcc gagacggttg atgagcgcgc
     1621 aatcttcggg caagcggtac aatcgcggct ttggatttcc gggttgggcc tgggccgtca
     1681 ccgcgccgat ttccatgaag ccgaatccga aagcggagat agttctgagc gcgactgcat
     1741 tcttatcaaa accgccggcg aggccgaccg gattagaaaa attcaaagaa gcaatttgca
     1801 cattgagccg tttgtcctca acccggtaaa agtgatcgag aggactgtga aggaattcca
     1861 atcgcgaaag taactcaagc gtcccatcat ggctttgctc cggatcttta ctaaacaacc
     1921 aaggacgaat gatagatttg tatagcatcg ttaatttatt tagccgaaag tttaccgctg
     1981 gaccggatgg ctgatttcgg aagctgaaag gtcgtttcga cgcaggggga gaaccttcgg
     2041 ttctccccgc gaatctaaat gccgcacacc gcttttttga acgacgccgg aggtatgtcc
     2101 tagcaagggt tatgaagatt acccagactg cagccgcaag atacagggct ctaagattgc
     2161 ctcaggcgac cgcaccgaat cgctatttaa aaggtgcgca cgccgatgga gtcatcaaca
     2221 tgttctcctg cataaccata tactgcctgg tttcag
//
LOCUS       DASUTG010000774         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_711076, whole genome shotgun sequence.
ACCESSION   DASUTG010000774 DASUTG010000000
VERSION     DASUTG010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711076"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF089_17950"
     CDS             complement(<1..717)
                     /locus_tag="VF089_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175898.1"
                     /translation="MAEKKTTFGLTLSNRALVTQGTQPKELIDMAVQAENSGAINAIW
                     VGDSILSKPRLECIPLLGAIAAQTSIVKLGVACMATIAQRNPVLLALQWASLDVLSGG
                     RTWLAACMGYPASQHPMAAKELEVMGIGSQERPARLEEIIQALRVLWSDEHASFQGKY
                     YSFQDVNLLPKPAQQPCPIFIAGTPRSNQIGDRGVERSLRRIARYADGWMTNQIELGR
                     FKDYHGRLRHMLLEEGRDPNA"
     gene            901..1785
                     /locus_tag="VF089_17955"
     CDS             901..1785
                     /locus_tag="VF089_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX6175899.1"
                     /translation="MKPAPFDYYAPLELQETLDLLERYGDEAKILAGGQSLMPLINLR
                     LARPRVIIDINRLHNLDAISTAPDGGLTIGALARQRTLERSTIIQEQNPMLAAAMPLI
                     GHFQIRNRGTIGGSLVHADPAAELPAMCLSLGGEFLLKSKSGARLIAAQDFFLGYLAT
                     ACQPGELLTEIRLPKWRAGEAWAVVEIARRKGDFALVGVALRAELDGVDILQDARIIM
                     FGVGGKPQRMEDGEALLKGCRIDQALLGELSRVVTEELDPDSDVHASAAYRKEVGGVL
                     VRRALLSALAKAKETVSH"
     gene            1782..2276
                     /locus_tag="VF089_17960"
     CDS             1782..2276
                     /locus_tag="VF089_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6175900.1"
                     /translation="MSAKQVIKVTVNGRTYERLVEVRMTLADFIRQDLDLTGTHLGCE
                     HGVCGACTILMDDEAVRSCLMLAVQADGAELRTVESLAQGDELHPLQRAFQDRHALQC
                     GFCTPGFLMTALAFLKDNPTPTREETRDAISGNICRCTGYQPIIDAILQASSESAKSN
                     DRST"
     gene            2277..>2919
                     /locus_tag="VF089_17965"
     CDS             2277..>2919
                     /locus_tag="VF089_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6175901.1"
                     /translation="MEPISKKAQGYVGMPIKRREDIRFITGRATYVDDIKLPNMLHAA
                     ILRSPRAHARISSMDTSKALQLSGVIKVFTFQDLGELAVPVPIRMYQLAGLERHLQPL
                     LAHDKVRYVGEPLALAIAESRYLAEDALDAIEVEYQDLPAVVDVYEALKDEVLVSDEA
                     GTNLAAVHEFSIGDVERAFQEAPYTRREEFRVHRFTGNPMETRGFVAAFDRGKG"
BASE COUNT          590 a          844 c          858 g          627 t
ORIGIN      
        1 ggcattcggg tcccgcccct cttccaacaa catgtgacgc aatcgcccgt ggtaatcttt
       61 aaaccttccg agctcgatct gattggtcat ccaaccgtcg gcgtaacggg cgattctccg
      121 caacgagcgc tccactccac gatcgccgat ctgattggat cgcggcgtgc cggcgatgaa
      181 gatcgggcaa ggctgttgcg caggtttggg caggagattt acatcttgga acgaatagta
      241 cttaccctgg aagctggcat gttcgtcgga ccagagaact cgcagagctt ggatgatttc
      301 ttccaatcgc gccggccgct cctggctgcc gatgcccatc acctcaagtt ccttggccgc
      361 catcggatgc tgactggccg ggtatcccat gcacgcggct agccatgtgc gcccgcccga
      421 taaaacatcc aggctcgccc actgcaacgc aagcagcacg ggattgcgct gcgcgatggt
      481 ggccatgcac gctaccccga gtttaacaat tgatgtctgc gccgctatcg cgccgagcag
      541 tggaatgcat tccaaacgag gcttgctaag aatgctgtcg cccacccaaa tagcatttat
      601 cgcgccgctg ttctcggcct gcaccgccat gtcgatcagt tccttgggtt gggttccctg
      661 ggtaaccagc gcgcggttcg atagggtcag accgaaggtt gtttttttct ctgccatgcc
      721 cgcgagcata ggacgagtcg gcgccatctt tcaagctgga tgtattatgc tcctcctgag
      781 tttctcgaag gatgagccgc gggtttcagc agaatccatt agagtgttgc gtctgacgta
      841 gcgctttcct cgccgatttg ccggttaccg ccgggtttga tatctgatcg atgacagcca
      901 atgaagccgg ctccttttga ctactatgcg ccgctggagt tgcaagagac cttggatctc
      961 ctcgagcgct acggcgacga ggcgaagatt cttgccggtg ggcagagtct catgccgctg
     1021 atcaatctac gcctggcgcg gccgcgcgtg attatcgata tcaaccgttt gcacaatctc
     1081 gacgcaatct cgactgcgcc cgacggcggc ctgacgatcg gggcgttggc gcgccagagg
     1141 accctggagc gctccacgat tattcaggag caaaatccaa tgctcgccgc ggcgatgccg
     1201 ctgatcggcc actttcaaat tcgcaaccgg ggtaccatcg gcggtagttt ggttcatgca
     1261 gatccagcgg ccgagctacc ggcgatgtgc ctgtcactcg gcggcgaatt cttgttgaag
     1321 agcaaatctg gcgcgcgtct gattgcggcg caggattttt ttctagggta cttggccacc
     1381 gcctgccagc caggggagtt gctcacggaa attcgtttgc ccaaatggcg tgccggcgag
     1441 gcttgggccg tggtcgagat cgcgcggcgc aagggtgatt tcgccctcgt tggcgtcgcc
     1501 ttgcgggccg aattggatgg tgtcgatatt ctacaagatg ctcgcattat tatgttcggc
     1561 gtgggcggaa aaccccaaag gatggaagat ggcgaagcgc tcttgaaagg atgccgcatc
     1621 gaccaggcgc ttttaggcga gctgagcaga gtcgtcaccg aagaacttga cccggattcg
     1681 gatgttcacg cttccgccgc ttaccgcaag gaagtcggtg gtgtgttggt tcgccgggca
     1741 ctgctatcgg cattggccaa ggcaaaggag acggtgagcc attgagcgca aagcaagtca
     1801 tcaaagtgac cgtcaatgga cgaacctatg aacgtctggt tgaagtaagg atgacgttgg
     1861 cggacttcat ccgccaggac ctggatctta ccggcactca cctcggctgc gagcatggcg
     1921 tatgcggcgc ctgcaccatc ctcatggacg acgaagcggt gcgctcctgc ctcatgctcg
     1981 cggtgcaagc cgacggcgcc gagttacgca ccgtcgagag cctcgcccaa ggtgatgagc
     2041 tgcatcctct acaacgagcc ttccaggacc ggcacgcgct tcaatgcggc ttctgcacac
     2101 ccggttttct gatgacggca ttggcctttt tgaaagacaa tccgacgccg acgcgggaag
     2161 aaacccgcga tgccatttca ggtaatatct gccgctgtac cggctaccaa ccgattatcg
     2221 acgcgatcct gcaggcgtcc tcggaatcgg caaaatcgaa cgaccggtca acctagatgg
     2281 aacccataag caagaaagct caaggttacg tgggtatgcc gatcaagcgg cgcgaagaca
     2341 ttcgcttcat caccgggcgg gcgacttacg tcgacgatat caagctcccc aacatgctcc
     2401 acgcggctat cctgcgcagt ccgcgggccc acgcaagaat cagttccatg gacacgtcga
     2461 aggctttgca gctttctggc gtgattaagg tgttcacgtt ccaagatctc ggcgagctgg
     2521 cggtccccgt gccgattcgg atgtatcagc ttgccggact ggaacgccac ctgcagcctc
     2581 ttctcgcgca cgataaggtc cgctatgtcg gcgagccgct ggccctggcc atcgcggaga
     2641 gccgatacct tgccgaggat gccctcgatg ccatcgaagt tgagtaccaa gatttgcccg
     2701 cggtagttga tgtttacgaa gcccttaaag atgaagtctt agttagcgac gaagccggaa
     2761 cgaatctcgc ggcggtgcat gaatttagca ttggcgatgt ggagcgcgcg ttccaagagg
     2821 ctccatacac ccgtcgggag gagtttcgcg tccatcgctt caccggcaat ccgatggaaa
     2881 ccaggggatt cgttgccgct ttcgaccgag ggaaagggg
//
LOCUS       DASUTG010000775         5653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_118775, whole genome shotgun sequence.
ACCESSION   DASUTG010000775 DASUTG010000000
VERSION     DASUTG010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118775"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF089_17970"
     CDS             <1..310
                     /locus_tag="VF089_17970"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX6175902.1"
                     /translation="ARVKDTLDDVLIDLEQEYGVRPRIVVTSARPGGRRTSFAQLQDM
                     LINTTHPFLIILGTGWGLTETIISQSDYVLEALEGWTDYNHLSVRSAAAIILDRLLGR
                     "
     gene            333..689
                     /gene="rplS"
                     /locus_tag="VF089_17975"
     CDS             333..689
                     /gene="rplS"
                     /locus_tag="VF089_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX6175903.1"
                     /translation="MNILDKLETQQSKQEIPALRAGETVRVHVKVVEGEKERTQVFEG
                     IVIGIGGKGNRANFVVRKISYGVGVERIFPFHSPRIDKVEVVSRGRVRRAKLYYLRER
                     SGKAARLFSDERSGSR"
     gene            complement(698..1066)
                     /locus_tag="VF089_17980"
     CDS             complement(698..1066)
                     /locus_tag="VF089_17980"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6175904.1"
                     /translation="MAEKTATSGTSPLVQGTVRTVLFPDSAISGRLSYDLPVKIDSKF
                     TGEVKASDLLVVGANAHVEARIAARNLQLEGKLIGSVQVIGCFEIMPGGHFKGDAQAA
                     ELRVHPGATFDGQGKITGEV"
     gene            1378..2703
                     /locus_tag="VF089_17985"
     CDS             1378..2703
                     /locus_tag="VF089_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6175905.1"
                     /translation="MDLFEPDQAKEQSAPAPLAERMRPRTLDEFVGQKHLLGPGRILT
                     EMVDSGQLRSLVLWGPPGSGKTTLAQILASKTGARCVHFSAVSSGVKDLKKIIDEAER
                     LRRVGKNTVLFVDEIHHFNKSQQDNFLPYVERGTIVLIGATTENPSFEVISPLLSRTQ
                     VLLLKTLSTEEIGAIVDRALRDNQRGLGTLHLNISPEGRDFLIEQCHGDCRIALNALE
                     TAATLAHNALAKEIGLIQVQEALQRKPLRYDKSGEEHYNVISAFIKSLRGSDPDAALY
                     WMMRMIEAGEDPLFIARRMVIFAAEDIGNADPNALQVAVAAKDAFHFVGLPEGKIPLA
                     QAVTYLATAPKSNASYLAMLAAMEDVEQRGSLPVPLHLRNAPTRLMKKLGYGKDYQYA
                     HNFPDHVVEQQHLPDELKTRRYYVPSDSGYEKQIKERLEGWQQKKITKS"
     gene            2871..4115
                     /locus_tag="VF089_17990"
     CDS             2871..4115
                     /locus_tag="VF089_17990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175906.1"
                     /translation="MQPVQLFCWHFMAYPYLAKNFDQVHETGWVTVPNSLWDNEKARG
                     LYQDYIDQLAYADELGFDGMVLNEHHQNIYGLMPSPNIIAAALTQRSTRGKIVVLGNL
                     LPLHMNPLRVAEEYAMLDQMSNGRLIAGFAPGGGPETFNYDVPSANSREKFWEAADLI
                     VRSWTEDGPFAHEGRYFPLRYVNPWPKPLQTPFPPVWIPGSRSPSTMTEVAKRGYCYF
                     LSSRSHGGETHRAQQEFAAVLEKHGDRYQPFRFGILMSAYVAESDRQAREECEEGVWY
                     FLKNCLKGHLRREGRQLTFGPGVPYIPAPAWNEYLKNYKPGRKLLGDVENWDELEASQ
                     SIIVGSPDTVYQRIWNLIERAKVGNLLIQFHIGNMPKDLALKSMTMFAGQVAPRLRES
                     SLSFFEQEFPGVNIEQHTEAHL"
     gene            4112..4927
                     /locus_tag="VF089_17995"
     CDS             4112..4927
                     /locus_tag="VF089_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6175907.1"
                     /translation="MSGRFLDLKQGKVWLLENGRGAPLLYLHGLVDVHSVKKTWMAFH
                     ERLATGARVIAPAHPGCSQSDENKDIETIEDVVFHYLEFLDALNMPQFDIVGSCVGGW
                     IAAEFAARHPEKIRKMVLIGAAGLFVQGALIGDVFMMAQPERGSSYAGLRQMLFSRHD
                     HPKALSLFPDGMGEIEDELRRYQMLRFGSRIGFKPPYFYNRPLRNRLHRITAPALVIW
                     GEHDRMVPLSHGETYAESMPQAQPLKVIPNAGHSVHVEEPELMAQIVSDFLAS"
     gene            4982..>5653
                     /locus_tag="VF089_18000"
     CDS             4982..>5653
                     /locus_tag="VF089_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175908.1"
                     /translation="MKNNVVLITGGAKGIGRHAALTLAQAGAKLALADTDVQRLHKTI
                     AEIKSRGGDAVAIPTDVRDEKDVRHMVYEVFDRFGRIDALINDAGIVPHFNWGIPRWP
                     RARFMSKDFWDSVIETNLGGTFLCTKYVVPFMEECRSGNIVNLWGGGRAENHGAAPYV
                     VSKDAIRTFTRFIAEEEREWNVCVVAFSPKQAIATEDAPEEARKRLPGPETLGNGFVL
                     AAQAGM"
BASE COUNT         1335 a         1569 c         1525 g         1224 t
ORIGIN      
        1 ggcccgagtt aaagacacgc tcgatgacgt cttgatcgac ttggagcagg aatacggcgt
       61 aaggcctcga attgtcgtga cgtcggcaag gcccggaggt cggcgaacct ctttcgccca
      121 gctccaagac atgttaataa ataccactca ccctttcctg atcattttgg gcacgggctg
      181 gggactcact gagacaataa tctctcaatc agattacgtt ttagaggcgc tcgaaggatg
      241 gaccgattac aaccatctct cggttcgatc ggcagcggcg attattctgg atcgtcttct
      301 gggacgttag ctttatagaa acaggagtag tcatgaatat tctagataaa ctcgaaaccc
      361 agcaatccaa gcaggaaatt cccgcgctcc gagccggaga aacggtgcgg gtgcatgtta
      421 aagttgtcga aggggaaaag gaacgaactc aggtctttga gggaattgtc attggcattg
      481 gcggaaaagg aaaccgcgca aatttcgtcg ttcgcaagat ttcttatgga gtgggcgtgg
      541 aacgtatctt cccgtttcat tctccacgga ttgataaagt cgaagtggtt tctcgcggca
      601 gagtgcggcg cgcaaagctt tattacttgc gggaaagatc aggaaaggcg gcgcggcttt
      661 tttccgacga acgatccggc agccgttaga cagcgcttta gacctcgccg gttatttttc
      721 cttgaccatc aaacgtcgcg cccgggtgaa ctctgagctc tgcggcttga gcatcgccct
      781 tgaagtgtcc tccgggcatt atttcgaagc aaccaatcac ctgaacgctg ccgatcaact
      841 tcccctctag ctggagattg cgcgcggcaa ttcgcgcttc tacgtgcgca ttggcgccga
      901 ccactaaaag atcgctagcc ttaacctcgc cagtgaactt gctgtcgatc ttaaccggca
      961 aatcatagct cagtctcccc gagattgccg agtccggaaa taaaacggtg cgcacagttc
     1021 cttggacaag gggagaggta ccactagtag ctgttttttc cgccatggct gcaatcctga
     1081 acggcgccta gaatagcatt cctagactcc caccgaaagt gaatctacag tcaagttgaa
     1141 tcacattgag gacaaagctg attgacgaac gcaccaccgg catcatcatc gcaaggctcg
     1201 agaaaccgaa ggaacatccc aaccacactc tgctgtttga gaattttcca cccgccggaa
     1261 tgtatcaatc ggcgttcgca aagcactgcc accgcgcagc cttcatgccg tatgcttgag
     1321 gtcgtttgaa ttgtgcccgg ctacaatgac gccaaacaat tgtgctagca tatcagcatg
     1381 gatttgtttg aacccgacca ggcaaaggaa caatcggcgc cagcacccct cgcagaacgg
     1441 atgcgcccga gaaccttgga tgaattcgtc ggacagaagc atctcctcgg gccgggacga
     1501 attttgaccg agatggtcga tagcggccag ctcagatcgt tggttctttg gggcccgccg
     1561 gggagcggta aaactacttt ggcgcaaatt cttgccagca agacaggtgc gcgatgcgtt
     1621 catttctcgg ccgtctcctc cggcgtcaag gacctgaaga aaatcataga cgaggcggaa
     1681 cggctccgtc gtgtgggcaa gaatacggtc ctcttcgtcg acgagattca tcatttcaac
     1741 aagtcacaac aagataattt tctgccgtac gtcgaacgcg gcacgatcgt tttgattggc
     1801 gccacaaccg agaacccgtc ctttgaggtc atctctcccc tgctttctcg cactcaggtt
     1861 ttgctgctca aaaccttatc gactgaggag atcggcgcga tcgtcgatcg tgccttgcga
     1921 gataatcagc gcgggctggg gaccctgcat ttgaacattt cgccggaggg ccgggacttc
     1981 ttgatcgagc aatgtcacgg tgattgccgg atcgcgctga atgcgttgga aaccgccgcg
     2041 actctggcgc ataacgcgct ggctaaagag atcgggctaa tacaagtcca ggaggcgcta
     2101 cagcgaaagc cccttagata tgacaagtca ggcgaagagc attacaacgt gatctcggca
     2161 ttcatcaaaa gcctgcgcgg cagcgaccct gatgccgctt tatactggat gatgcgcatg
     2221 atcgaagcag gtgaggatcc tttgttcata gcgcgtcgga tggtgatctt tgccgccgag
     2281 gatatcggca atgcggaccc caacgcattg caagtcgcag tggcggccaa ggatgctttt
     2341 catttcgtcg gcctgccgga aggtaagata ccactcgcgc aagcggtaac gtatctagcc
     2401 acagcgccga aatccaatgc ctcgtatttg gcaatgctcg cagcaatgga ggacgtagaa
     2461 caacgcggct cgttgcctgt tcctctccat cttcgcaacg cgccgactcg cctgatgaag
     2521 aaattaggat acggcaaaga ttatcagtac gcccacaact ttcccgatca tgtggtcgag
     2581 caacagcatc taccggacga actgaaaaca cgaagatact atgtgccgtc agattccggg
     2641 tacgagaagc agatcaaaga gagactcgag ggctggcaac agaaaaagat aacgaaaagc
     2701 tagcagacgt gaagtgatcg aacggtcccg cagccatgaa tccctcctac agaaccccgg
     2761 ccatacctat cagacgtgat tctcttgttg cggcattcca tcgctttata gtatgacctt
     2821 gctattctga agaacaatcc acgatagtct cgcattttaa acgaggcaca atgcaacctg
     2881 tccaattatt ctgctggcat ttcatggcat atccatatct ggccaaaaat tttgaccagg
     2941 tccacgagac cggctgggtc acggtcccca acagcctttg ggataacgag aaagcgcgcg
     3001 gattatacca ggactatatc gaccagctgg catatgcgga cgagctcggt tttgacggca
     3061 tggtgctcaa cgagcaccat caaaatattt atggtttgat gccttccccc aacatcatag
     3121 cggctgccct aactcaaagg tccacccgcg gcaagatcgt cgtgctcggc aatctgctgc
     3181 cgctgcacat gaacccgctt cgagtcgctg aggaatacgc gatgctcgat cagatgagca
     3241 acggccggct gatcgcgggt ttcgcgccgg gcggcggccc cgaaactttt aattacgacg
     3301 taccctcggc caatagccgc gagaaattct gggaagcggc ggatctgatc gtgcgttcct
     3361 ggaccgagga tggtccgttc gcccatgagg gacggtactt tccactgcga tatgtaaacc
     3421 cttggcccaa gccgctgcaa acaccctttc ctcccgtatg gatacccggc tcgcgcagcc
     3481 cgtcaactat gaccgaggtg gccaagcggg gctactgtta cttcctttcc tcacgcagtc
     3541 acggcggcga aacccatcgc gcccaacaag agtttgccgc cgtgttggaa aaacacggcg
     3601 atcgttatca gccattccgc ttcggcatct tgatgtccgc gtacgtggcg gaatccgacc
     3661 ggcaagcacg cgaggagtgt gaagaaggcg tttggtattt cttgaagaac tgtttgaagg
     3721 gtcacctgcg ccgggaaggt aggcagctta ctttcggccc cggcgttccc tacattccgg
     3781 cgccagcttg gaacgaatac ctcaaaaatt acaagccggg ccgcaagctt ctgggcgacg
     3841 tagaaaactg ggacgagctc gaggcttctc aatcgatcat cgtcggcagc cccgataccg
     3901 tataccagcg catctggaat ctcatcgaac gagccaaggt tggaaacttg ttgatccaat
     3961 ttcacatcgg caacatgccc aaggatctcg cgttaaagag catgacgatg ttcgctggac
     4021 aagtcgcgcc gcgcctgcgg gagagttctt tgagcttttt cgaacaagaa tttccaggcg
     4081 ttaacatcga acagcacaca gaggcgcatt tgtgagcggc cgctttctgg atctcaaaca
     4141 aggcaaagtc tggcttctgg agaacggccg tggcgctccc ctcctttacc tgcacggctt
     4201 agtcgatgtc cactccgtta agaaaacctg gatggccttc cacgagcgtc tcgctacagg
     4261 agctcgggtc attgcgcccg cgcatccagg atgctcgcaa agcgacgaga acaaggacat
     4321 cgaaaccatc gaagacgttg tctttcacta tctcgagttc ctggatgcat tgaacatgcc
     4381 gcagttcgac atcgttggca gctgcgtcgg cggctggatt gcagcagaat ttgcggcgcg
     4441 ccatccggaa aagattcgca agatggtttt gatcggggcc gcaggactgt tcgttcaagg
     4501 ggcattgatc ggtgatgtct tcatgatggc tcagccggag cgcggctcca gttacgcagg
     4561 gctgcgccag atgcttttct cgcgtcacga tcacccaaaa gctttgagcc tttttcccga
     4621 tggcatgggt gagattgaag acgagctgcg tcgctatcag atgctgcgct tcggctcgcg
     4681 tatcggcttc aaaccgccct acttctataa tcggccgttg cgcaaccgcc tacatcggat
     4741 cactgcgccc gccctggtga tctgggggga acacgaccgt atggtgccgt tgtcgcatgg
     4801 cgagacttac gccgagtcaa tgccgcaagc acagccgctc aaggtaattc ccaacgcagg
     4861 ccacagcgtg cacgtcgaag agcccgagtt aatggcacaa atcgtttcag attttttggc
     4921 aagctaatca gagaagccca actgaagaca gaccgcagcc acaggtcgca ttgggaggtt
     4981 tatgaaaaat aatgtcgttc tcattaccgg tggtgccaaa ggcatcggtc ggcacgcggc
     5041 actgacgtta gcccaagctg gcgccaagtt ggcgctggcc gacaccgatg tccaacgctt
     5101 gcacaagacg atcgccgaaa tcaaaagtcg cggcggggac gcagttgcta ttcccactga
     5161 cgtgcgcgat gaaaaagacg tgcggcatat ggtgtatgaa gttttcgacc ggttcggccg
     5221 cattgacgca ttgatcaacg acgccggcat cgtgccgcat ttcaactggg gcattccgcg
     5281 ttggccgcgc gcccgcttca tgagcaagga tttctgggac agcgtcatcg aaaccaattt
     5341 gggcggcacc tttctttgta ccaagtacgt tgtccccttt atggaagagt gccgttccgg
     5401 caacatcgtc aatctttggg gcggcggcag agccgaaaac cacggcgccg cgccctacgt
     5461 cgttagcaaa gacgccatcc gcacatttac gcgctttatc gccgaagaag agcgcgagtg
     5521 gaacgtctgc gtggtcgcct tcagccccaa gcaagccatc gccactgaag acgcgcccga
     5581 agaagcgcgc aagcgccttc ctggtcctga aactctcggc aacggattcg tgctcgccgc
     5641 ccaagcgggc atg
//
LOCUS       DASUTG010000776         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_711184, whole genome shotgun sequence.
ACCESSION   DASUTG010000776 DASUTG010000000
VERSION     DASUTG010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711184"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1245
                     /locus_tag="VF089_18005"
     CDS             94..1245
                     /locus_tag="VF089_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6175909.1"
                     /translation="MYYPDLSGFGVDNDRLTSQRLSRLQQAMKQHDLAALMLTDWLNI
                     RYATNSVFMMNLRATAIQRFVLVPQSGAPLICQRELSSRARGSFRRFDAFMFGMRPPV
                     ATRDFAAQAVANLRDLGITGERVGVDCLNLEAVEALRDAGIKLVDGSRAINQARAVKT
                     TDELQLIRWTTRAKEKGYDLVRAALHEADLPEERLSRIMLDSLLDQGFEAGSEFVSIY
                     ESSKARTHNEPMGTDMVVVEGDLVICDATVAGPGGYYSDFARTFSRGTPTAQSRNRYR
                     EAYQSLQEALPLIKPGPCTELFNRFGKHSSSRMAGLDGFHGVGLCIYESPWLRGGDPP
                     EYVVDLEENMIVALEINHYPVKLEHLLRITTSGAEILSRYPVDPELLPA"
     gene            1442..2356
                     /locus_tag="VF089_18010"
     CDS             1442..2356
                     /locus_tag="VF089_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6175910.1"
                     /translation="MSRLVSAPQEYRASGLAIWSALLAVYLIWGSTYLAIRFTVETIP
                     PFVSAAFRFIVSGAILYTWRRFAGDAKPTAVEWRSAATVGIFLLLGGNGGVVWASQFI
                     PSSLAALLVATVPLWMVAIDSARPGKPRPSLAAVTGILIGFGGVVLLIRFAASDALVF
                     NLFGTTAVLFASLSWAIGSLYARTAKLAASPLLATGMEMFSGGVALLLIAIVTGEWDQ
                     LDIGAVSSRSALALAYLTAVGSAAFVAYVWLLRVAPTPLVSTYAYVNPLVAVLLGYFL
                     ANEPMTGRTLFAASLIIGSVVLVSAPKR"
BASE COUNT          444 a          681 c          675 g          558 t
ORIGIN      
        1 ctcagctcat cgaacgtcgt gcgcctcgcc tctgcatctt tttgagcagc ctgccatcag
       61 agtttcaata gcgagaaatc caaaggagag ttcatgtatt acccagacct gagtggcttc
      121 ggggttgaca acgaccgtct cacatcgcag cgtttgtcgc gtttgcagca agccatgaaa
      181 caacacgatc tggcggcgct catgctcacc gactggctca atattcgcta cgcgaccaac
      241 tcggttttca tgatgaatct ccgcgccact gcgattcagc gtttcgtctt ggtgcctcaa
      301 agcggggctc cattgatttg ccagcgcgag ctttcgagca gagctcgagg ctcgtttcgc
      361 cgcttcgatg cctttatgtt cggcatgcgg ccgccggttg caaccaggga cttcgccgcg
      421 caagcggtag cgaacctgcg cgacttgggt attactggcg agagggtcgg cgtcgattgc
      481 ctgaacctcg aagcagtcga agcattgaga gacgccggca ttaaactggt cgacggctct
      541 cgcgcaatca atcaggcgcg cgcggtaaag accaccgatg agctccaact gattcgctgg
      601 accacccggg ccaaagagaa aggctatgat ctggttcgcg cggcgctgca tgaagcggat
      661 ctgcctgaag aacgcttgag ccggatcatg ttggattctt tgctggatca gggattcgaa
      721 gcgggcagtg aattcgtttc gatctatgaa tcgtccaagg cgcgaacgca caacgagccc
      781 atgggcacgg acatggtggt ggtggaaggt gatctggtga tctgcgatgc caccgtcgcc
      841 ggacccggag gttactacag cgacttcgcc cgtactttta gtcgcggcac gccaacggcg
      901 cagagccgga accgttatcg agaagcttac caaagcttgc aggaggcatt gccgctgatt
      961 aaacccgggc cttgcaccga gcttttcaac cgcttcggca aacatagctc ctcgagaatg
     1021 gctggactgg atggttttca tggggtcggt ttgtgcatct atgaatcgcc ctggctgcgc
     1081 ggcggcgatc cacctgagta cgtggtggac ttagaggaaa atatgattgt tgccctcgag
     1141 atcaatcact acccggtgaa gctcgagcac ttgctgcgca taaccacttc gggagcggaa
     1201 attttgtcgc gctatccggt agatccagag ttactcccgg cgtgagaggc aaacgcgcaa
     1261 ttccccaact tctgttcggc aatatcacag cccttctcgc gacgatcacg gcgcagttca
     1321 ttgttccacg gtttcgcgcc ggttggccaa atcagtttat ctcagctcat ccttcagctc
     1381 cggaacctgc gagaattcga agattgctca atcgactgat caaaccggaa agaaccttcc
     1441 ggtgagccgg ttagtatcgg caccgcagga ataccgagcc tccggcctgg caatctggtc
     1501 ggcattgttg gcggtttacc tgatctgggg ttccacctat ttggcaatcc gattcacggt
     1561 cgaaacaatt ccaccgtttg tctccgcggc ttttcgcttc atcgtctcag gagcaatctt
     1621 atatacctgg cgtcgcttcg ccggtgatgc caaacccacg gcagtggaat ggcgcagcgc
     1681 cgccaccgtc ggaatttttc ttctgctcgg cggcaacggc ggcgttgtct gggcctcgca
     1741 gttcattcct tcgagtctcg ccgcgttact ggtagccacg gtacccttat ggatggtcgc
     1801 gattgactcg gcccggccgg gcaaaccaag accgagcctt gctgccgtta ccggtattct
     1861 catcggcttc ggcggggtcg ttctgctgat ccgcttcgcc gccagtgatg ccctcgtctt
     1921 caatctgttc ggcaccacgg ctgtgctgtt tgcgtcgctc tcatgggcca tcggttcgct
     1981 gtatgcgagg accgcaaagc ttgcggcgtc accgctactg gcaaccggca tggaaatgtt
     2041 ttccggcggt gtcgccctgc tgttgatcgc gatcgttacc ggtgaatggg accagctgga
     2101 tatcggcgcg gtttcatcac gctcggcgct cgcgctggcg tacttgacag cggtgggttc
     2161 cgcggccttc gttgcctatg tatggctcct gcgggtggcg cctacgcctc tggtatcgac
     2221 ctacgcttac gtcaatccgc tggtcgcagt tttgctcggt tactttcttg ccaacgagcc
     2281 tatgacaggg cgcacgttgt ttgctgcaag tctgatcatc ggctcggtgg tgctggtcag
     2341 cgcgcctaaa cgatagcc
//
LOCUS       DASUTG010000777         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_858517, whole genome shotgun sequence.
ACCESSION   DASUTG010000777 DASUTG010000000
VERSION     DASUTG010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858517"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1173)
                     /locus_tag="VF089_18015"
     CDS             complement(43..1173)
                     /locus_tag="VF089_18015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6175911.1"
                     /translation="MDFTLSEEEKLIQSTAVQFVKKELLTREGDYLRQRELFLPPGDP
                     PRRQLDADVQQTLTGIAQRLGLWTLELPESGAEPIGYLARVLVYREFGRSILPFGPTC
                     APALLAKTDFAKDLGAGKLSLALAFDQIHKTGTLDGINTRYRPGSAGFSISNSEITIY
                     DPSADLFLVPAREQGTHRIGLFLIQKNSAGISIEDEVDLTNDAAMARLNLRDLEVPES
                     RLLGYEYEAGEILAAEQLRIAARCLGIGMRCLADSIEHARTRETFGRTLAQRQAIQWM
                     LADSSVDLRSCTWLTMEAASRADQSLPFFDAAALAKKRAAAMAFSAADTAIQIHGGYG
                     VCKEFPFEGFYRETRMLRLMCGREAELNRAMGQTFLKVGLKR"
     gene            complement(1220..2416)
                     /locus_tag="VF089_18020"
     CDS             complement(1220..2416)
                     /locus_tag="VF089_18020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6175912.1"
                     /translation="MDFRLSPDEETLRKTVEEFVRERLIPLEAQFEDAPDIFEGSRWK
                     SRARLSRDPVILRYIEMMEDLEKKAEAQGLWHLDVPKEYGGMEVSNVAMLAVTEELEK
                     TAIPFEFGNHVSNILYNCKGEQIDRFLWPCIRGEKTSAFGLTEPASGADPSMLQTTAA
                     PDGDHFIINGTKMFPTFADVADFVQLFARLPNTKGREGVTCFLIETGTPGYRVTRSIE
                     TIAGSEPCELLFEDCRVPRNQVLGEVGNGWALNQAWLGARRFQVGIRAHGMAQRVLRV
                     VAQLLGSDSAEASKFVERIGYFLGELEALRTLSYFGAWKADQKMDARSEAANVKLFGT
                     ELLHKIVDFALETAGPAMLRKSHVLARYFRHARARRIIEGPSEIQRHIIQRALFREGV
                     AGLELP"
BASE COUNT          623 a          819 c          721 g          604 t
ORIGIN      
        1 cctagtaggt ctttagatag acttttctga atctcctgcg ggctagcgct tcaggcccac
       61 cttcaagaac gtttggccca tcgcccggtt cagctcggct tcacgcccac acatcagacg
      121 cagcatccgc gtttcccgat aaaacccttc gaacgggaac tccttacaga cgccgtagcc
      181 accatgaatc tggatcgcgg tatcggcggc gctaaaagcc atggccgcgg ctcttttctt
      241 cgccagcgcc gcggcgtcaa aaaacggcaa gctctgatcg gcgcgcgatg cggcttccat
      301 cgtcaaccac gtgcagctgc gaagatccac tgacgagtcc gccagcatcc actgaattgc
      361 ctggcgttgc gctaacgttc tgccgaaggt ttcgcgagtt cgcgcatgtt cgatggagtc
      421 agcgaggcag cgcatgccga tcccgagaca gcgggcggca atgcgcagct gctcggccgc
      481 gagaatctct ccggcttcgt attcgtaccc gagcaggcgg ctttcgggaa cttcgagatc
      541 acgcaggttc agccgcgcca tcgccgcatc gtttgttaga tcgacttcat cctctatgga
      601 gattcctgcg ctattctttt ggattagaaa caaaccgatt cgatgggtcc cctgttcccg
      661 ggccgggacc aggaacaaat cggcggatgg atcatagatg gtgatttcgc tgttgctgat
      721 gctgaagcct gcggatccag gtcgataacg agtattgatt ccatccaggg tgccggtttt
      781 gtgaatctga tcaaatgcca aggctaggga gagcttgccg gctcccaagt ctttggcgaa
      841 atcggtttta gccaacagcg ccggagcgca agtcggcccg aacggtagga tgctccggcc
      901 aaactcgcgg tagaccagaa cgcgggccaa atagccgatc ggttctgccc cggactcggg
      961 cagctcaagg gtccataaac ctagccgttg cgcaatcccc gtgagtgtct gctggacgtc
     1021 cgcatccaat tgacgacgcg gtggatctcc cggcggcagg aacagttcgc gctgcctgag
     1081 ataatcccct tcgcgagtga gcagctcttt tttgacaaat tgaacggctg tgctctgaat
     1141 cagcttctct tcttcggaca gtgtgaaatc cataattatc gctaactcaa agcttgtcga
     1201 agttcctcac gccgctgcat tacggcaact caagaccggc aacaccttca cggaaaagcg
     1261 cccgctggat gatatggcgc tgtatctcgg aaggaccttc gatgattcgc cgagcccgcg
     1321 cgtggcggaa gtaacgcgcc aacacatggc tcttgcgcag cattgccggc ccggcggtct
     1381 ccaaagcgaa atcgacgatc ttgtgcaata gctcagtgcc aaacaatttc acgttggccg
     1441 cctccgagcg agcgtccatt ttctgatcgg ctttccatgc cccgaaatag ctcaacgtcc
     1501 gcaacgcttc caattcgccc agaaaatagc cgattcgctc gacgaattta cttgcttcgg
     1561 ccgagtcgct gccgagcagc tgcgcgacaa cgcgcagaac tcgttgtgcc atgccgtgag
     1621 cgcgaatgcc gacttgaaaa cgacgcgccc ccaaccatgc ctgattgagc gcccaaccgt
     1681 tgccgacctc gccgagaacc tgattgcgag gcacacggca atcttcgaag agcagttcgc
     1741 agggctcgct gccggcgatc gtttcgatgc tgcgggtcac gcgataccct ggggtcccgg
     1801 tctcaatcag gaaacaggtc acaccttccc tgcccttggt attcggcaag cgggcaaaca
     1861 gctgcacgaa atctgcaaca tcagcgaagg tgggaaacat cttggtgcca ttgataatga
     1921 agtgatcgcc gtccggcgcc gcggtggttt gcagcatcga aggatcggct ccagaagcag
     1981 gttccgtcaa gccgaacgcc gacgtcttct cgccgcgaat gcacggccaa agaaatctgt
     2041 cgatctgctc acctttgcag ttgtagagaa tattggagac gtgattacca aactcaaacg
     2101 gaatcgcggt tttttccagc tcctcggtaa ccgcgagcat cgccacattg ctcacttcca
     2161 tgccgccgta ctcttttgga acgtcaagat gccaaagccc ttgagcctca gccttcttct
     2221 cgagatcttc catcatttct atgtagcgca ggatcaccgg atcgcgactc aatctggcgc
     2281 gactcttcca gcggctgcct tcaaagatgt cgggagcatc ttcgaactgc gcttccagcg
     2341 gaatcaagcg ctcgcgaaca aactcctcga cagttttccg caacgtctct tcgtcgggtg
     2401 ataagcgaaa atccaccttc gattcctcag gattcggcga ttacttgatg accggatacg
     2461 taaccgattc tgcaatgtcc agctcccctt accacacctg aaaataaacc aaaagataaa
     2521 atcatttatt ttcacagatg cagtaactaa cgctgtccac atgaggactg attgaatgtt
     2581 caacagatct atttgggcag aacatgttga ggtaacgaac tattgactcg tgacacttcg
     2641 cagtgagcag cgggcaggcg agctgagcca ctcacgaatg aatacttccc agcccagcga
     2701 agaactccta gagccaatac ttgggaatta gccgatctga ccagtcgctt tgagccacga
     2761 gcggttg
//
LOCUS       DASUTG010000778         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1154404, whole genome shotgun sequence.
ACCESSION   DASUTG010000778 DASUTG010000000
VERSION     DASUTG010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154404"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="VF089_18025"
     CDS             <1..2214
                     /locus_tag="VF089_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6175913.1"
                     /translation="QTNEELATKAQQLAEQNAEVERKNQEIEQARHALEEKAAELALA
                     SKYKSEFLANMSHELRTPLNSILILAQQLTSNPDRNLTTKQVEFARNIHASGGDLLNL
                     ISDILDLSKIESGTVTVEPEEVLFSKVQEAVERSFRHVAEARHLTFKIETHTELPQAM
                     ITDLKRLQQVLKNLLSNAFKFTTHGSVVFRMRVAKAGWSAEHPILKKSSRVIAFEVSD
                     TGIGIPADKQRIIFEAFHQADAGTSRRYGGTGLGLAISRELATLLGGEIRLVSNVNEG
                     SSFTLYLPEDYFASASGDFQSRWATFTPAPLMVQHSLKPEEIADDRETILPKDAVVLI
                     VEDDPRFAGVLLDLVRERGFKGVVTNKGSIAHNLAREFNPVAILLDIFLPDMLGWTVL
                     SNLKQDSTTRHIPVQIVSVEEERIQGLGHGAFSYLIKPATSGELEIAIDRVLGYARSD
                     RKELLIVEDNAVERQSIVELLSHEKVEITTAHSGGDAWNILLSKKFDCMVLDLKLPDI
                     TGFDLLERIRRQRDLQDLPIVVFTGKDLTEQDELQLAQMAESVVIKGVQSPERLLDET
                     SLFLHLVTTELAPAKQQMLERLRHSDEALRGKTVLVVDDDVRNIFALTSLLEQHGVRV
                     ISAETGKEAISLLDREEVIDAVLMDIMMPDMDGYETMGLIRKSTKHKLLPILALTAKA
                     MKGDREKCLEAGASDYIAKPVNTEELLGLLRIWLFGKQTNSGVGYERSNREHPRS"
     gene            2180..>3242
                     /locus_tag="VF089_18030"
     CDS             2180..>3242
                     /locus_tag="VF089_18030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6175914.1"
                     /translation="MNEATANILVVDDDVKTLAAMEALLAGPGRNISTASSGTEALRL
                     LLRQDFALILLDVRLPVMDGFETAALIRQRERFRYTPIIFISAIDTLESDVFRGAASG
                     AVDYLFKPVVPQILQAKVSVFVDLFHKNEQLKLQAIRQSEERFRMVVESLQDYAIFMM
                     DPSGRVSTWNLGAERIVGWSQHEAIGKLFGAFYTPEDEAKGLAGQALKESAGVGRYEE
                     EGWRLRKDGSRFWANVVVTSLVDDHGELVGFSAIFRDLTARKTTEEQLKQLNSDLEER
                     YVEKAAELAHTIGEREKLQEQLLQVQKMESIGTLAGGIAHDINNILNVISGYANLILQ
                     GSDDKDRNPEHVKVIIETVE"
BASE COUNT          808 a          853 c          892 g          689 t
ORIGIN      
        1 caaaccaatg aagagctggc aaccaaagct caacagctcg ccgaacagaa cgccgaagtg
       61 gagcgcaaga atcaggaaat cgaacaggcg cggcatgctc ttgaagagaa ggccgccgag
      121 ctggctctcg cctcaaagta caaatcggaa tttctcgcca acatgtcgca cgaattgcgc
      181 acgccgctca attctatcct catcctggcg cagcagctga catccaatcc cgatcgaaat
      241 ctcaccacga agcaggttga gtttgcccgc aatatccacg cctccggcgg cgatctgcta
      301 aacttgatca gcgacattct cgatctttcg aaaatcgaat ccggtaccgt cacggtggaa
      361 cctgaggagg ttctcttcag caaagtgcag gaagcggtgg aacgaagctt ccgccatgtg
      421 gcggaagcgc gacatcttac ttttaagatt gaaacgcaca ccgaactgcc gcaagccatg
      481 atcaccgacc tcaagcggct ccagcaggtg ctgaaaaatc tgctttccaa cgcgttcaaa
      541 tttacgactc atggaagcgt tgtcttccgc atgagagtgg ctaaggcggg ttggagcgca
      601 gaacacccca tactcaaaaa aagctcgaga gtgatcgctt ttgaggttag cgatacgggt
      661 atcggcatcc cggccgacaa gcagagaatc attttcgagg cttttcacca agccgatgct
      721 ggaaccagcc gtcgctatgg cggcaccggt cttgggctgg ctatcagccg agaactcgcg
      781 accttattgg gcggagagat ccggcttgtc agcaacgtta atgagggcag tagctttaca
      841 ctctatctgc cggaagacta ttttgcctcg gcatcgggcg attttcagtc gagatgggcg
      901 acgtttacgc cggctcccct gatggtgcag cattcgctta aacctgaaga gatcgccgat
      961 gatcgcgaaa cgattctacc gaaggacgcg gttgtcctta tcgttgaaga tgatcctcgc
     1021 ttcgccgggg tgctattgga tttggtgcgc gagcgaggct tcaagggtgt ggttaccaac
     1081 aagggatcga tcgcccacaa tctggcgcgc gaattcaatc cggttgccat cctgttggat
     1141 atcttcctgc ccgacatgct cggatggacc gtgttgagca acctaaaaca agactcgact
     1201 acgcgccaca tcccggtgca aatcgtcagt gtcgaagaag agcgcataca agggctgggt
     1261 cacggcgcct tctcttattt gattaaaccg gccaccagcg gtgagttgga aatcgccatt
     1321 gaccgcgtgt tgggctatgc caggtcagac cgcaaggagc tcctgatcgt ggaagacaac
     1381 gcggtagaaa ggcagagcat cgttgaactg ctctcccatg agaaggtcga aatcactacg
     1441 gcgcacagcg gtggcgacgc ttggaacatt ttgctctcaa agaagtttga ctgcatggtt
     1501 ctcgacttga agctgccgga catcaccgga tttgatttgc tcgagcgtat tcggcgtcaa
     1561 cgagatctgc aagatttgcc gattgtcgtt tttaccggca aagacttaac ggagcaagat
     1621 gaactgcagc tggcacaaat ggccgagagt gtggtcatta agggcgtcca atctcccgaa
     1681 cgtttgctcg acgaaacatc cttgttcctg cacctggtaa caaccgaact cgcacctgcc
     1741 aagcaacaaa tgctcgaacg gctgcgccac agcgacgagg cgctcagggg taagacagtg
     1801 ctggtggtgg acgatgacgt gcgcaatatt ttcgccttga ccagcttact cgaacagcat
     1861 ggagtgcgcg tcatcagcgc ggaaaccggc aaagaggcaa tttcgctcct cgatcgagag
     1921 gaagtgattg acgccgtcct gatggatatc atgatgccgg acatggacgg ctatgaaacc
     1981 atgggcctga tccggaaaag cacaaagcac aagctactgc ctattctcgc gctaacagct
     2041 aaggccatga aaggtgaccg cgaaaaatgc ctggaagcag gcgcttcgga ttatatcgcg
     2101 aagccggtca acacggagga actgctcgga ttactacgca tctggctttt cggcaaacag
     2161 actaattccg gagtcggtta tgaacgaagc aaccgcgaac atcctcgtag ttgacgacga
     2221 tgtaaaaacg ctcgcggcta tggaggcgct tctcgccggc ccggggcgaa acatctcgac
     2281 ggcttcttcg ggtacggagg cgctccgcct gttgctccga caggactttg cgctgattct
     2341 tctggacgtg cgcctgccag taatggacgg ctttgagact gccgccctaa ttcgacagag
     2401 agaaaggttt cgctataccc caatcatttt tatctctgct atcgacactc tggaatcgga
     2461 cgtttttcga ggagccgcca gcggggctgt tgattacctc ttcaaacccg tcgtgccaca
     2521 aattcttcag gctaaggttt cggtttttgt cgacctattt cataagaacg agcagctaaa
     2581 actacaagct atccggcaaa gcgaggagcg ctttcggatg gtggtcgaaa gcttgcagga
     2641 ttatgcgatt tttatgatgg acccgagcgg ccgcgtctca acctggaatc tcggcgccga
     2701 gcggatcgtc ggatggagcc agcatgaagc cattggcaaa ttgttcggcg ccttctacac
     2761 gcccgaagac gaggcaaaag gcctggctgg ccaggcactg aaggagtcgg ccggggtggg
     2821 acgatatgaa gaggagggct ggcgcctgcg caaggatggc tctcgctttt gggctaatgt
     2881 tgtggtcacc tcgttggtcg atgatcacgg cgagctcgtc ggattttcgg ccattttccg
     2941 tgacctcact gcccgcaaga caaccgaaga acaacttaaa caactcaact ccgatctcga
     3001 agaaagatat gtggagaagg cggccgagct agcgcacact atcggtgaac gggaaaagct
     3061 gcaagagcag cttctccaag ttcagaaaat ggaaagtatc ggcactttgg ccggaggaat
     3121 tgcccacgac atcaacaaca ttctcaacgt tatatccggc tacgctaatc tgattctgca
     3181 gggtagcgac gacaaagaca gaaatcccga acatgtaaag gtgattatag aaactgtcga
     3241 ac
//
LOCUS       DASUTG010000779         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1154495, whole genome shotgun sequence.
ACCESSION   DASUTG010000779 DASUTG010000000
VERSION     DASUTG010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154495"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1130
                     /locus_tag="VF089_18035"
     CDS             117..1130
                     /locus_tag="VF089_18035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175915.1"
                     /translation="MNRNTPKSNQWNKASGIIAVALLTLLLGSYAFAQSVRVGRTVGG
                     SGFHIPSYVAVDKGLFKAEGLDPSFIAATAGVLVRATIAKEIEFTPIPGGGSEAMLKG
                     APLAFFVGQSLISQWTLTTTPAIKRVEDLRGKILGLGRPGSADYSEIVITLGKHFKMQ
                     PGKDYRVISFTGEPDRIAALIQGSIQGGVLSFPHAARAEKEGMKILMRTGDYIPRLGG
                     TFLTHRDFIKEKRDIVKRFTRGIIKAEDYIRTNKKGTMEVIEKYFEIKDPKVVEGIYS
                     QVYDKYSPEMPPDLIQDLFESRTTPELGWPAGKPLPDLNQFVDRSIVNEVLKEMGRKP
                     GGK"
     gene            complement(1161..1958)
                     /locus_tag="VF089_18040"
     CDS             complement(1161..1958)
                     /locus_tag="VF089_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175916.1"
                     /translation="MNPRKIFAEHESLIFGTGFIILLLVLWESIPLWYTLPRGMALFF
                     TTPSRIAASFYELLLDGTIEKHFYVSAVAFLAGLGLSIIVGLPLGLLMGRSATLEQLL
                     DPYVTAANATPRIVFLPLLILWFGIGIWSKILIVFVGALFPLLINTYVGVKNVNRVLV
                     NVVRSFGANEWQVMKIVVLPNSVPYIIAGLRLAIGRAILGVVVGEFFGSSHGLGYMIA
                     SAATNYKVDVVFVGVAIFMAMSVFLTLAIKQLESRLASWRPEAAKSF"
     gene            complement(1959..2747)
                     /locus_tag="VF089_18045"
     CDS             complement(1959..2747)
                     /locus_tag="VF089_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6175917.1"
                     /translation="MLGQAFFGEHRRLWVALGSVLFLIGSWEFVLTYIFPLDPFFFTK
                     PSLIGVAFKEQVQGSKLWHDLAVSSKAFLWGFTFAVIVGIPVGLVMGWRKRVEYALDP
                     FLTALYASPLVALAPLFIIVFGVGVLGKAALVFLLAIFPFIFNTFAGVKSTDALLINV
                     IRSFGGTEKDLYLKVILPSTMPYIIAGARIAIGRGLVGIIVGEFYAASEGIGFAISQA
                     GDTYRLPDMFVGIIILSLIAVILTELMRKLELTLAPWRASDEVR"
     gene            complement(2750..3526)
                     /locus_tag="VF089_18050"
     CDS             complement(2750..3526)
                     /locus_tag="VF089_18050"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013100127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175918.1"
                     /translation="MAAFIKAHDVSLVFKAKDREPVTALSNFSLEIAQGEFVSIVGPS
                     GCGKSTFLNIVLGLIEPASGVIQLNGTRISGPGQERAMVFQEFGLLPWRSVTANVELG
                     LELKGVPSAKRAARAKELINLVGLEGFENHYPHELSGGMKQRVGLARALATEPEVLLM
                     DEPFAALDAQTRDLMQSELLQIWERSKKTVLFVTHSIEEAAYLSDRVIVMTARPGRTK
                     DVIQIDLPRPRDYEMRLTPKFNEIKLRIWEVLREELTSRG"
BASE COUNT          946 a          965 c          951 g          763 t
ORIGIN      
        1 agaaaatccc tctttgtgca aaacatggtg gcccaataca acggcttgcg tacacgccag
       61 gcctaggatt atgctaggct cgctgcagcg atcttcgtat atgagaggag atgtttatga
      121 atcggaatac gccaaagtcc aatcaatgga ataaagccag cggaatcatc gcagttgcac
      181 tgctaacact gctgctcggc agttacgcat tcgcgcaatc cgtcagggtc ggcaggaccg
      241 tcggcgggag tggctttcac atcccatcct acgttgccgt ggataagggc ctcttcaagg
      301 cggagggact ggaccccagt ttcatcgctg caaccgcagg tgtgttggtc agagcgacca
      361 ttgccaaaga gatcgagttc acaccgattc ccggcggcgg ttcggaagca atgctcaaag
      421 gcgcgccgct ggcttttttc gtcggacaat ctttgatctc acaatggacg ttgacgacga
      481 caccggccat caagcgagtc gaggatcttc gaggtaaaat tttgggcctg ggccgccccg
      541 gctcggccga ctacagcgaa attgtcatca ccctcggtaa gcacttcaaa atgcaacccg
      601 ggaaagatta tcgagtgatc agcttcacgg gcgagcctga ccgcattgcg gcgctcattc
      661 agggctccat tcagggcggc gtgctgtcat ttcctcatgc ggctcgagcc gaaaaagagg
      721 gcatgaagat tcttatgcga accggcgatt atatcccgcg cctgggtgga acatttctca
      781 cgcatcggga tttcatcaaa gaaaagcggg acatcgtcaa acgcttcacc cgaggtatta
      841 tcaaagccga agattacatc aggaccaaca agaaagggac catggaggtc atcgagaagt
      901 atttcgagat caaggatccc aaagtggtcg aaggcatcta cagtcaggta tatgacaagt
      961 acagccccga gatgccgccc gatttgatcc aggatctctt cgaatcccgg acgactcccg
     1021 aactcggctg gccggcggga aagccgctgc cggatctgaa ccagttcgtt gatcgcagca
     1081 tcgtcaatga agttctaaag gagatggggc gaaagccggg aggaaagtaa tccatcgaaa
     1141 cacacatctg accactagct ctagaagctc tttgccgctt cagggcgcca acttgccaac
     1201 cgggattcca gttgtttgat agctagggtt aaaaaaaccg acattgccat aaagatcgcg
     1261 acgccgacga agacaacatc gactttgtag ttggttgccg cagacgcgat catgtaaccc
     1321 aagccatggc tggagccgaa aaattcaccg acaaccacac caagaatcgc tctacctatg
     1381 gccaacctca gcccagcaat aatgtaagga accgaattgg gcagcactac gattttcatc
     1441 acctgccatt cgttagcgcc aaacgatctc acgacattga ccagaactcg gttgacgttc
     1501 ttgacgccga cataggtgtt gatgagcaac ggaaacagtg cgccaacaaa gacaatcaag
     1561 atcttcgacc aaatgccgat gccgaaccat agaatcaata atggcaaaaa gacaatcctc
     1621 ggggtggcat tggctgcggt cacatatgga tctaataatt gctcgagagt cgctgacctg
     1681 cccatgagga gccctaacgg cagcccgaca attatggata aacctagtcc cgccagaaag
     1741 gcgactgcac tgacgtagaa gtgtttttca attgtgccat cgagaagcag ttcataaaag
     1801 gaagcggcga tgcgtgacgg cgtggtgaag aagagcgcca ttcccctggg cagggtgtac
     1861 cagagtggaa tgctttccca aaggacaagt aataggatga taaaaccggt tccgaatatg
     1921 agagattcgt gctctgcaaa gatctttcgc gggttcattc agcgtacctc gtcacttgcg
     1981 cgccacggcg caagcgtgag ctcaagtttg cgcatcagct cggtcagaat gaccgcaatc
     2041 agcgacagaa tgatgatgcc gacgaacatg tcggggagcc ggtaggtatc gccggcttga
     2101 gagatggcaa aaccgattcc ttccgaagcg gcatagaatt ccccgacaat gatcccaacc
     2161 aaccctcggc caatcgcaat gcgcgcgccg gcgatgatgt agggcatcgt gcttggcaga
     2221 atgactttca agtagagatc cttctcagtc ccgccgaatg atcgaataac gttgattaac
     2281 agcgcgtcgg tcgacttcac gccggcaaat gtgttgaaaa taaaggggaa gatcgcgagc
     2341 aagaaaacca gtgctgcttt accgagcacg ccaacgccaa aaacgataat gaacaagggc
     2401 gctagagcaa ccagtgggct cgcataaagc gcggtcagga agggatcgag tgcgtactcg
     2461 actcgttttc gccaccccat gaccaggcca acaggaatcc cgacgatcac agcgaaggtg
     2521 aagccccaca gaaaagcctt gctgctcacc gccaagtcgt gccacagttt gctcccttgc
     2581 acttgctctt tgaacgccac gccgatcagt gaaggtttgg taaagaagaa tggatccaac
     2641 gggaagatat aggtcaaaac gaattcccag gatccgatca gaaataacac ggagccgagt
     2701 gcgacccaga gtcgccggtg ttcgccaaag aaagcttgtc caagcatatc tatcctcgag
     2761 aggtcagctc ttcgcgcaag acttcccaga tgcgaagctt gatttcatta aatttgggcg
     2821 taagtcgcat ttcataatct ctcggtcttg gcaaatcgat ctgaatgacg tctttagtcc
     2881 ggccgggtct tgccgtcatg acgatgactc ggtctgagag ataggcagcc tcttcaatgc
     2941 tgtgggtgac aaagagcacg gtttttttag agcgttccca gatctgcagt aattccgatt
     3001 gcatcagatc gcgcgtctgg gcatcgagtg cggcaaaggg ctcatccatg agcagcactt
     3061 ccggctcggt tgccagcgcg cgagcgaggc cgacccgctg tttcatgccc ccggagagct
     3121 catgcggata atggttttca aagccttcca atccgaccaa attgatcagt tctttcgctc
     3181 gtgctgctct ttttgcagac ggaactccct tcaattcaag tccgagttcc acgttggctg
     3241 tgacgcttcg ccagggaagc agcccgaact cttgaaaaac catcgctcgc tcctggcccg
     3301 gaccgctaat gcgcgtgccg ttcaactgaa ttacgccgct ggcaggttca atcagcccta
     3361 aaacgatatt gaggaaggta ctttttccgc aacccgacgg gccgacgata ctgacaaact
     3421 cgccttgggc aatctcgagg ctgaagttac tgagtgccgt gacgggctct cgatccttgg
     3481 ctttgaagac tagagagacg tcgtgagctt taatgaaagc tgccatggga acccttactc
     3541 tcgccaacag cgcatctgca gctacagcgt ggttgatgat acagcgctac tatagccata
     3601 attcactgta cgatcactgt cgaat
//
LOCUS       DASUTG010000780         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1599453, whole genome shotgun sequence.
ACCESSION   DASUTG010000780 DASUTG010000000
VERSION     DASUTG010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599453"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /gene="tgt"
                     /locus_tag="VF089_18055"
     CDS             complement(<1..1571)
                     /gene="tgt"
                     /locus_tag="VF089_18055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX6175919.1"
                     /translation="MKGLSFTLEKQSSGSKARAAKFLTLHGEVETPIFMPVGTQATVK
                     GQTLDSLTAAGARVLLVNTYHLLLRPGTDVLKSFGGVHNFMNWRGPVLSDSGGFQIFS
                     LPHSREISEEGARFQSYVDGKSFLLSPESSIETQKAIGSDIMMALDVCIPATAPYAQA
                     RSAMEITHRWAERSLCAGDDSDQALFGIVQGACHTDLRRISAEFLSRLPFDGLAIGGL
                     AVGETHRERYAVTALVIGSLPEKLPRYLMGVGTPIDILESVHRGVDMFDCIIPSQLAH
                     RGVAFTSRGKLQMRRSVYRLSQEPLDTQCDCSSCRQHSRGYIHHLIKCDELLGWHLLG
                     VHNMTFYQRLMSEMRQSILADDFLSYYETKRFELTRSDEDNPVSHPRKSAKASQPTRL
                     GDYVIHTSAENLYSIRQISSGEIMHSVNAPDDEATRLYVEQSCLASRLLKQESERNEL
                     VIWDVGLGAAFNAMAVLHCFERSFAKPGGQAHRRLRLVSFEWDFDPLRLALRNPSRFP
                     HLRHGAPIQILHSGK"
     gene            complement(1899..>2597)
                     /locus_tag="VF089_18060"
     CDS             complement(1899..>2597)
                     /locus_tag="VF089_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX6175920.1"
                     /translation="KIQANMDPRHRDRMAFLRILSGRFEKDMIAYHPRLEREVRLARA
                     YRIFAREREPVREAFAGDIIGVINPGLFVIGDTVTTGEPAELIDIPRFPPEHFAVLIN
                     QEIVRYKQFHKGLSQLEEEGAVQVMYAAAGTRREPILAAVGALQFDVVLARLRQEYGV
                     EARIERLPFTCARWIQGQNIERMTLGSGVLKCRDHNGETVVLFASDWHLEYFRKENPH
                     VEIVKVGSPWMTKN"
BASE COUNT          570 a          748 c          720 g          559 t
ORIGIN      
        1 catttaccgc tgtgaagaat ctgtataggc gcgccgtgcc gaagatgtgg aaagcggctc
       61 gggtttctta gagcaagccg gagcggatcg aaatcccatt cgaagctgac caggcgcaag
      121 cggcgatgcg cctgtccgcc aggtttagcg aaacttcttt cgaagcagtg aagaaccgcc
      181 atggcgttga acgccgcccc gaggccgaca tcccagatta ccagctcatt tctctcgctc
      241 tcctgtttga gcaaccggga ggcaagacac gactgctcca catacagtct ggtggcttca
      301 tcatccggag cattcaccga gtgcatgatt tcgcccgagc tgatctggcg gatgctgtac
      361 aagttttccg ccgacgtgtg gattacataa tcgcccaggc gggtgggctg ggatgccttc
      421 gcgctttttc gcgggtggct cacgggattg tcttcgtcgg agcgtgtgag ctcgaatcgt
      481 ttcgtctcgt aatacgacaa aaaatcatca gccaaaatac tctgtctcat ttcgctcatc
      541 agtcgctgat agaaagtcat gttgtgaacg cctagtagat gccagcctaa gagctcgtcg
      601 cacttgatca gatggtggat gtagccgcgc gaatgctgtc ggcagctgct gcagtcgcac
      661 tgcgtatcta ggggttcctg tgacaatcga tacaccgatc tgcgcatctg aagcttgccg
      721 cgcgaagtga aagccacgcc tcggtgggcc agttgagagg ggatgataca gtcgaacata
      781 tcgacgccgc ggtgaacgct ctccagaatg tcgatggggg tgcccacacc cattagatag
      841 cgagggagct tctccggcag gcttccgatg acgagcgcgg tcacagcgta gcgctcgcga
      901 tgagtttcac ctaccgccaa acctccaata gccagtccgt caaaagggag gcggcttaag
      961 aattcagcgc tgatccttct gagatcggta tgacaggccc cctggacgat tccaaacaga
     1021 gcttgatccg aatcgtctcc tgcgcagagc gaacgctcgg cccaccgatg ggtgatctcc
     1081 atggccgatc gggcttgagc atagggagcg gtcgccggga tgcaaacgtc caaggccatc
     1141 atgatatcgc tgccgattgc tttctgcgtc tcaatgcttg attcgggcga caggagaaaa
     1201 gattttccat cgacataact ttggaaacga gcgccttctt cgctgatctc tcgtgagtgt
     1261 ggcagagaaa aaatctgaaa cccgcccgaa tcgctgagca ccggcccacg ccagttcatg
     1321 aaattgtgga cgccgccaaa gctcttcagc acgtctgtcc ctgggcgcag cagcaaatga
     1381 tacgtattga caagaagaac ccgcgcgcca gcggccgtca aactgtcgag tgtctgccct
     1441 ttgacggttg cctgggttcc gacaggcatg aagatcggag tctcgacctc gccatggaga
     1501 gtgaggaact ttgccgcccg tgccttggat ccgctggact gcttctccag agtgaagctc
     1561 aaccctttca cgaaccgcaa ctctcgcccc gcttgacaga ttcaagcggc gggcattttt
     1621 ctttcatgag gtggtacttc tatgtttcaa tcgggtcgca cgatcaccac gccggcaccc
     1681 tgcgaaaatg cgcgatccgc gtgctgagcg tggcgtcatc tcctcaccgc aaggggtgag
     1741 cttctccaga gagtgagccc gatgttgcca acggaggcgc agatcgttgg gaccacggcg
     1801 gtcaggtcgg cagaattcgg ccgagggact cgacatgttt aatggcgtcg taaaagatcg
     1861 tgcaagacag gccaacggcc gcgccacggt cggtgttctt agttcttcgt catccagggc
     1921 gaacccacct tgacgatttc aacatgcggg ttctctttgc gaaaatattc aagatgccaa
     1981 tctgacgcga aaagcaccac ggtctcgcca ttgtgatcac ggcactttaa tacgccgctg
     2041 cccagagtca tccgttctat attttggccc tggatccagc gagcacatgt gaatggcagg
     2101 cgctcaattc tggcctcaac accatactct tggcgtaagc gcgccaaaac aacatcgaat
     2161 tgtaaagctc ccacagccgc gaggatcggc tcccgccgcg tgcccgctgc ggcgtacatg
     2221 acctgcaccg cgccctcttc ctccaattgt gacaacccct tgtggaactg cttataacgg
     2281 acgatctcct gattgatgag aactgcgaaa tgttccgggg gaaaacgtgg aatatcgatg
     2341 agctcggccg gctctccggt ggttactgtg tcgccgataa cgaacagacc cggattgatg
     2401 acgccgatga tatcaccggc aaacgcctcc cggaccggct cccgttctcg ggcaaagatc
     2461 cgatacgctc gcgccagccg cacctcccgc tccaaccgtg ggtgataggc gatcatgtct
     2521 ttttcaaaac ggccggacag aatgcgcaga aaggccatcc ggtcacgatg ccgcgggtcc
     2581 atattggctt gaatctt
//
LOCUS       DASUTG010000781         7318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1009584, whole genome shotgun sequence.
ACCESSION   DASUTG010000781 DASUTG010000000
VERSION     DASUTG010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009584"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VF089_18065"
     CDS             <1..1410
                     /locus_tag="VF089_18065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6175921.1"
                     /translation="PTPDFSGHLRDYQREGLGWLAFLQRFGLGGCLADDMGLGKTVQV
                     LALLESRRDLYVRGERQAPRPSLVVVPRSLVFHWKREATRFTPKLRILDHTGSLRAKP
                     GEHFSEYDVVITTYGTLRRDALEFKPQHFDYCVLDEAQAIKNAGTLSAKAARVLRAEH
                     RLAMSGTPVENHLGELWSLFEFLNPGLFGSATVFDRAGRNPDPATRSLLARALRPFVL
                     RRTKSQVARELPAKTEQTIFCDLEGREKKQYEELRAYYRARLLKDFEHGDRSRAKFQA
                     LEALLRLRQAACHPGLIDKSKVAEPSAKIDTLMAQLEQVRDEGHKALVFSQFTSLLAI
                     VRDRLDRDKVAYAYLDGRTREREARVEEFQNDPNIKLFLISLKAGGLGLNLHAAEYVY
                     LLDPWWNPAVEAQAIDRAHRIGQTRQVFAYRLIARNTVEEKVLELQKSKRDLADAIIT
                     ADNSLLHNLTREDLELLLS"
     gene            1675..3390
                     /locus_tag="VF089_18070"
     CDS             1675..3390
                     /locus_tag="VF089_18070"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175922.1"
                     /translation="MTYDLLIKGGRIYDGSGLPSYLGDVAVKDGRIVETGRINGSAQR
                     VVNADGLAVSPGFIDFHTHLDAQLLWDPLATSSCYHGVTTVIPGNCGLSLAPCKEQDR
                     EVILKSFERVEAITLPALKAGVKWGFTAFGEYLDALRGKLGINVASLVGHCALRQFVM
                     GEASIERAATPAEIHQMKEVLKASVRAGASGFSTNQNPVHMYADGTPIQSRFATDEEI
                     IELACALGEINQGAVQISRGSLGVSVPMRESVQLFDEISSRSGRPVIWQSITHRWDKP
                     NEWRQLLDLAKESIDRGVQSYPLCNARLFNNRLTMKNAQVFDDLPTWKTILFLPLQAR
                     IEAIKDPETRKKMRYEAVEEKKSSRFSRRWDLVYLINAVKPENKHLEKKSVTEIAKIR
                     GQDVIDAFLDLSLEEGLDTEFQTSSTNGDEEAVAEIIRSPYVLVGQSDAGAHLIYDAG
                     FGYSTRLLGYWVREKEIMSLEEGVRKLTFMVASIFGLQGRGLLRRGMAADLCIFDPTT
                     IRECEPEMVQDLPGNEKRFIQKATGIEMTIVNGEVLVEKDEHTGALPGSVLGSGNGAT
                     LQAAA"
     gene            3422..4144
                     /locus_tag="VF089_18075"
     CDS             3422..4144
                     /locus_tag="VF089_18075"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175923.1"
                     /translation="MYYGARGIVGLVKPTYRPGSMESFIKLMPEGVGFIPAHAGIRAG
                     NEKELQEALTVAEKKVARLAELGADVVMIMGAPPTMVRGHGADRAIADSLTKKYKIPV
                     TVATIAQVDAFRALGMKKLVGITYFQDDMNQRFAKFLQDDGFAVKAMKGIEVPFKDAG
                     KLSPEAIYNFAKKAFIEHGPADGIYMLGAGWHNLPVIEMLEKDLKTTVVSSIPAQVWA
                     TKKILHLNEPVAGYGRLLAEMP"
     gene            4169..5179
                     /locus_tag="VF089_18080"
     CDS             4169..5179
                     /locus_tag="VF089_18080"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6175924.1"
                     /translation="MRRFSILTVVIFMLSLVLWTAASTVMAQSVTEKAEAEGKLMFYA
                     TFNAADSKMLTDGFKQAYPKIDAAFYRGTDAAIMERILTENRAGQNLWDVAVTTSFYG
                     HNLTKRGLFVPYDSPERKFFKDGYKDQHGAWTSVYTNYAAFGYNTGTVPKGSVPQSYA
                     DLLKPEWKGNIGMDSKAYEWFGTILKAMGEEKGLAYMRELAKQTQLRSGRTLIAQLVA
                     AGEFKGALTAYSQTFEVLKPSGAPVDWVYLNPVFANIHPAGISAKAPHPNAARVFIDF
                     ILSKRGQEIVRKMNRIPDRTDTPPAQPRLIQGIKPAFAPMEVLEDFSRYGKMFDDIFA
                     AR"
     gene            5232..6008
                     /locus_tag="VF089_18085"
     CDS             5232..6008
                     /locus_tag="VF089_18085"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6175925.1"
                     /translation="MKVVVRFLTKEFASGAGNVRALGGVDFEVRDREFFGIIGPTGCG
                     KTTLLHIIAGLEQPTTGSVEFIGEKRTKSIVSMVFQESALMPWRSVEENVPLGAEFRN
                     EHPSMLKRVSEFFLEVVRLLDFGKARPYELSGGMKQKVAIARALANDPEVILMDEPFA
                     SLDAQTRLLMREELLRIWERDKKTVILVTHNIEEAVMLCDRIAVMSSAPALIKSIITV
                     DVPRPRNFRSMKDPDFARCTEKIWDLLRYDVDQAMQRNSA"
     gene            6160..>7318
                     /locus_tag="VF089_18090"
     CDS             6160..>7318
                     /locus_tag="VF089_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175926.1"
                     /translation="MYLIPAYQAMLPIEDPDIWWHLRTGQWIIAHGHVPIEDPFSIYG
                     AGKPWIAYSWLFEVLVYELYRYFGLTGLVAFTVTMSLVVALTIHLLLRRSRLPFVAEV
                     LLLAVIFASLKPLLSPRPWLFSILFTAIELILIGILRRSWKLLPALLLPLLFVLWANL
                     HIQFVYGLAILTFLTFESIVNKLPSSGLPRSHSQALSVTHSALLLGACLLATLVNPYH
                     YGIYLAIFEYVTQSGAFQNISELHPMFFRSLADWLVLSLTLTGAFILGWQRRWNIFYL
                     VLFVTAACFAFRARRDVWFVALVVAAIVSEFATTENYSDYFEFTRERVAAVILSLLIT
                     LYGLSYLRDVSENSLAKVVELHFPVKAVNFIRSSQLPGPIYNSLDWGGYLIW"
BASE COUNT         1612 a         1920 c         2093 g         1693 t
ORIGIN      
        1 ccgacacctg acttcagtgg gcatttgcgc gactatcaac gcgaggggtt gggctggctc
       61 gcatttctgc agcgcttcgg gttgggtggt tgcctggccg acgacatggg gttgggcaaa
      121 acagttcagg tgctggcgct cttggaatcg cgccgcgatt tgtatgttcg tggcgagcgc
      181 caagcgccgc ggccttcatt ggtcgtggtg ccgcgctcgc tggtcttcca ttggaagaga
      241 gaggccacgc gattcacgcc taaactgcga attcttgatc acaccggatc gctgcgcgca
      301 aagccgggcg aacatttcag cgaatatgat gtcgtcatta ctacctacgg cacgctccgg
      361 cgcgatgcct tggaatttaa accccagcat ttcgactatt gtgttcttga tgaagcacag
      421 gcgatcaaaa atgccggaac attgtccgcc aaagcggcgc gcgtgctgcg cgccgaacac
      481 cgcctggcga tgagcggcac tccggtcgaa aaccatctgg gcgaactgtg gagtttgttc
      541 gagtttctca accccgggct cttcggcagt gcgaccgtat tcgaccgggc cgggcgcaat
      601 cccgaccccg ccacccgtag tttattggcg cgcgcgctac ggccatttgt gctcaggcgc
      661 accaaaagcc aggtggcgcg cgagcttccg gcgaagaccg agcaaacgat tttctgcgat
      721 ctcgaggggc gcgaaaagaa acagtacgaa gaattgcgcg cctattaccg tgcccggttg
      781 ttgaaagact tcgaacatgg ggatcgcagc cgagccaaat tccaggcgct cgaggctctg
      841 ctgcgcctgc gccaggccgc ttgccacccg ggtctgattg acaagagcaa agttgccgaa
      901 ccgagcgcca aaatcgatac tttgatggcg caactcgagc aagtgcgcga cgagggccat
      961 aaagcgttgg ttttctctca gttcaccagt ttgcttgcca tcgtccgtga tcggctcgac
     1021 cgtgacaaag ttgcttatgc ctaccttgat ggccgtactc gcgagcgcga ggcgcgggtg
     1081 gaggagtttc agaacgatcc gaacatcaag ctatttctca tcagcctcaa ggcaggcggg
     1141 ctgggactca atttgcacgc cgctgagtac gtctaccttc tagatccgtg gtggaaccca
     1201 gccgtagaag ctcaggccat cgatcgcgcc catcgcatcg gccaaacccg ccaggtcttt
     1261 gcttaccgcc tgattgcgcg aaacaccgtc gaagaaaagg tgctggagct gcagaaaagt
     1321 aaacgcgact tggccgacgc catcatcacc gcggataaca gcttgctgca caatttgacc
     1381 cgcgaagatc tcgagctctt actctcctga acttgatacg gttaaaaggt caaggtcatt
     1441 agttaaatcg gcgtctttgc atttgccact acactgaatc gatttagaca acggccgaga
     1501 tctccgtgtg tcgttcacgc gaaaatattg atagatacat agaacgtaag gcggttgtag
     1561 tcaccgttgt tcgatctgga ggcgtcaagt cttctcgtta gcatgcccat ttgacatttg
     1621 gcggcgcgtg acaataagct cggtgaaagg tcggtggatc aaggaggatc tctcatgacg
     1681 tatgacttac tcatcaaagg cggccgcatt tacgatggca gtggattgcc ttcgtatctc
     1741 ggggacgtgg cggtcaagga cggccgcatc gttgaaacgg ggcggatcaa tggcagcgcg
     1801 cagcgagtcg tgaatgccga cggccttgcg gtgtcgccgg gtttcattga ttttcacacg
     1861 cacctcgatg ctcagctttt atgggatccc ttggccactt cttcctgcta ccacggcgtg
     1921 accacggtta taccgggaaa ttgcgggctg tcgctggcac cctgtaagga acaggaccgg
     1981 gaggtcatac tgaagagctt cgagcgggtt gaagcgatta cgctgccggc tctaaaggcc
     2041 ggggtgaagt ggggttttac cgcgttcggg gaatacctcg acgctctccg cggtaaactg
     2101 gggatcaatg tcgcgtcgct ggtaggccac tgcgctttgc gccagttcgt tatgggagag
     2161 gcgtccatcg agcgcgccgc gacaccggcg gaaattcatc agatgaagga agtgttaaag
     2221 gccagcgtgc gtgcaggcgc ctccggcttt tcgaccaatc aaaatcccgt tcacatgtac
     2281 gccgacggca cgccgatcca gagtcgcttt gccaccgacg aagagattat cgagcttgcc
     2341 tgcgcgctcg gcgaaatcaa ccagggtgcc gtgcagattt ctcgcggctc gctgggtgtt
     2401 tcggtgccca tgcgcgagag cgtgcagcta ttcgacgaaa tttccagccg ctccggccgg
     2461 ccggtgatct ggcagagcat cacgcaccgc tgggacaagc ccaatgaatg gcgtcagctt
     2521 ttggatcttg ccaaagagtc gatcgaccga ggcgtgcaat cctatccctt gtgtaatgcg
     2581 cgtttgttca ataaccggct cacgatgaag aatgctcagg tctttgatga cctgccgacc
     2641 tggaagacaa ttctgttctt accgctccaa gcccgcatcg aagcgatcaa ggatccggag
     2701 acgcgcaaga agatgcgcta tgaagcggtg gaagaaaaga agtcgtcccg cttctcgcgc
     2761 cgctgggatc tggtttatct catcaacgca gtcaaaccgg aaaacaagca cctggaaaaa
     2821 aagagcgtga cggaaatcgc caaaatccgc ggccaggatg tgatcgacgc tttcctcgat
     2881 ctctcgctcg aggaaggatt ggataccgag tttcaaacct ccagcaccaa cggcgatgag
     2941 gaagccgtgg ccgagattat ccgcagccct tacgtgctcg tcggccagtc tgatgccggc
     3001 gctcatttga tctacgatgc cggctttggt tactcgaccc ggctgctggg ctactgggtg
     3061 cgcgagaaag aaatcatgag cctcgaagaa ggcgtgcgca agctgacgtt catggtcgca
     3121 tcgattttcg gcctgcaggg ccgaggtttg ctgcgccgcg gcatggcggc ggatttgtgc
     3181 atcttcgatc cgacgaccat tcgcgaatgc gaaccggaga tggtgcaaga cttgccgggc
     3241 aatgagaaac gtttcatcca gaaggcgacg ggcatcgaga tgacgatcgt caacggcgag
     3301 gtcctggtag aaaaagacga acataccggc gctctgcccg gcagtgtgct cggcagcggc
     3361 aacggcgcca cgctgcaagc ggcggcataa aagagctcca gcgttaagga gaaaataata
     3421 catgtactac ggcgcgcggg gaatcgtcgg gttggtaaag cccacgtatc gtccgggatc
     3481 gatggaaagt ttcatcaagc tgatgccgga aggtgttggc tttattccag cgcatgccgg
     3541 tattcgcgcc gggaatgaaa aagaactgca ggaggctttg acggttgccg aaaagaaggt
     3601 ggcgcgtttg gcggaattgg gcgcggatgt ggtgatgatt atgggggcgc caccgacgat
     3661 ggtccgcggc cacggtgcgg accgcgccat cgccgattcg ctgacgaaga aatataaaat
     3721 tcccgtcacc gtggcgacca tcgcgcaggt ggatgcgttc cgcgcattgg gaatgaaaaa
     3781 actcgtcggt attacttatt tccaagatga tatgaatcaa cggtttgcaa aatttctgca
     3841 ggacgacggt ttcgcagtca aagcgatgaa aggcatagag gtgccgttca aagatgccgg
     3901 caagctttcg ccggaagcga tctataattt cgccaagaag gcgtttatcg agcacggacc
     3961 tgcggacggc atctatatgc tcggcgccgg ttggcacaac ctgccggtga tcgagatgct
     4021 ggaaaaagat ctgaagacta cagtggtttc gagcattccc gcgcaggtat gggcgaccaa
     4081 gaaaattctg cacttaaatg agccggtagc gggctacggg cgattgctgg cggagatgcc
     4141 gtaactaggc gcggggagtg ttgaagtgat gagaaggttt tcgatcctga cggtcgttat
     4201 tttcatgctc agcctcgtat tgtggacggc agcgtcaaca gtgatggcgc agagcgtaac
     4261 ggaaaaggca gaagctgaag gtaaactgat gttttatgcc accttcaatg cggctgactc
     4321 aaagatgctg accgatggat ttaagcaagc ctatcctaaa atcgacgccg ctttttatcg
     4381 cggcaccgac gcagctatca tggaaaggat tctgaccgaa aatcgcgccg ggcagaatct
     4441 gtgggatgtc gctgtgacga caagcttcta tggtcacaat ttaacaaaac gaggtctgtt
     4501 tgttccctac gattcacccg agcgcaagtt tttcaaagac ggttacaaag accagcacgg
     4561 agcgtggacg tcggtctata ccaactacgc ggctttcggc tacaataccg gtaccgtgcc
     4621 caaaggcagc gtaccccaat cttacgctga tctgctgaag cccgagtgga agggtaacat
     4681 cggcatggat agcaaagcgt acgagtggtt cggcacgata ctcaaagcta tgggcgagga
     4741 gaagggtttg gcttacatgc gtgagctcgc caagcaaacc cagttgcgta gcggtcgcac
     4801 gctgatcgcg caactcgtcg cagctggaga attcaaaggc gcgctgactg cttattcgca
     4861 gacgttcgaa gtgttgaagc ctagcggtgc tccggttgac tgggtttact taaatccggt
     4921 tttcgccaat attcatcccg ccggcatttc cgcaaaagcg ccgcatccca atgcagcgcg
     4981 ggtgttcatc gatttcatcc tatctaaacg aggccaggaa atcgtacgca aaatgaacag
     5041 aattcccgat cgtacggata caccgccggc gcagccgcgc ctgatacagg gtatcaagcc
     5101 ggctttcgcg ccgatggaag ttcttgaaga cttctctaga tacggcaaaa tgttcgacga
     5161 tatcttcgct gcacgttagc gcttggagtc aaccagcgtt ctccgaccaa gattcaacag
     5221 gtaagtcatc gatgaaggtt gttgtccgtt ttttaactaa agagtttgcc tctggagcgg
     5281 gcaacgtccg cgccttgggc ggtgtcgatt tcgaggtgcg ggaccgggag ttttttggca
     5341 ttatcgggcc caccggctgt ggcaaaacca cgttattgca catcatcgca ggcctcgaac
     5401 aaccgacaac cgggtccgtc gaattcatcg gcgagaaaag aaccaagtcg atcgtctcca
     5461 tggtttttca ggaatcggcg ctgatgccgt ggcgcagcgt cgaggaaaac gtcccgctcg
     5521 gagccgagtt tcgcaatgag cacccatcga tgttgaaacg ggtcagcgag ttttttctag
     5581 aggtagtccg gctgctggat ttcggcaagg cccgacccta cgagctttcc gggggcatga
     5641 aacaaaaggt cgctatcgct cgggccctgg ccaatgatcc ggaagtgatt ctcatggatg
     5701 agccgttcgc ctcgctcgat gctcagaccc gactgttgat gcgcgaagag ctgctgcgca
     5761 tctgggagcg tgacaagaaa accgtcattc ttgtgacgca taatatcgag gaagcggtca
     5821 tgctctgcga ccgtatcgcc gttatgagct cggcccccgc cttgatcaag agcattatca
     5881 ccgttgatgt gccgcggccg cggaattttc gcagcatgaa agatcccgac tttgccagat
     5941 gcacagaaaa gatttgggac ttgctgcgct acgatgtcga tcaagcaatg cagcgaaatt
     6001 ctgcttgata tgttccgccc gcgccggaag tagccaaccg gccaactcca tatcaatcct
     6061 gatccgctgc ttacacgttt gacgttaaag ttgactagag cgacgactag aagagttctc
     6121 agtaaggaaa cgatctgccg cttagccata tggactctgg tgtacttgat ccccgcctac
     6181 caggcgatgc tgccaatcga ggatccggat atctggtggc acttaaggac tgggcagtgg
     6241 atcattgctc atggccatgt cccgatcgaa gacccattct cgatttacgg cgcgggaaag
     6301 ccgtggatcg cctatagctg gctattcgaa gtcctggttt acgagctata cagatatttc
     6361 ggtttgactg ggttggttgc gtttaccgtt acgatgtccc tcgtcgtggc gctgacgatc
     6421 catctgctgc ttcgtcgatc ccgcttgcct tttgtcgctg aagtcctttt gctggcggta
     6481 attttcgcca gcctaaaacc cctgctgagc cctcggccgt ggctcttctc gatcctattt
     6541 actgccatag agctgatact catcgggatt ttgcggcgct catggaaact tcttccggcc
     6601 ttacttttgc cgctgttatt tgtcctctgg gccaatttgc acattcagtt tgtctacggc
     6661 ctagctatct tgacgttttt gacattcgag tcgatcgtca ataaattgcc gagctccggt
     6721 ttgccgcgga gccatagcca agctttgtcg gtgacgcatt ccgcgctact tcttggtgcc
     6781 tgtttgcttg caaccctggt gaatccttac cactacggta tttacctggc gatcttcgaa
     6841 tacgttacgc agagcggagc tttccagaat atttctgaac tgcatcccat gttctttcgt
     6901 tcgctcgccg actggttggt tctttctttg actctaacgg gggcatttat attgggttgg
     6961 cagaggcgct ggaatatttt ctatttggtt ttgttcgtga cggcggcttg ttttgccttt
     7021 cgcgcgcgcc gcgatgtctg gttcgtggct ttagtggtgg cggccatcgt tagcgagttc
     7081 gctaccacag aaaactacag tgattatttc gagttcacga gggaaagagt agcggccgtg
     7141 attctttctt tactcataac tttgtacgga ctgagctatc ttcgcgatgt ctcagaaaat
     7201 tctctggcca aagtcgtcga attgcatttt ccggtgaagg cagtgaactt catcagatcc
     7261 agtcagcttc cagggccaat ctataattct ctcgattggg gaggctatct gatttgga
//
LOCUS       DASUTG010000782         4160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1303290, whole genome shotgun sequence.
ACCESSION   DASUTG010000782 DASUTG010000000
VERSION     DASUTG010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303290"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF089_18095"
     CDS             complement(<1..268)
                     /locus_tag="VF089_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175927.1"
                     /translation="MKQRFQRGDPDVFDLAWLDDQELEALRGAMLSNGRNEICAYWMR
                     SFNEQSAIRTGKLRIIPGTEARDPNFNQFSISELDAARSIFGGLE"
     gene            complement(477..830)
                     /locus_tag="VF089_18100"
     CDS             complement(477..830)
                     /locus_tag="VF089_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175928.1"
                     /translation="MNIISKVSTGKASLFTTYWLIFFPIAFVIKLADEAGTAGEQLLL
                     ILLIPFLWSLYGVWRCAFNVRHLIWGYVARGLIVVNVLLFFAVFFASMANEIAKPQPA
                     KAEDVRQQRGFLTLS"
     gene            1478..1981
                     /locus_tag="VF089_18105"
     CDS             1478..1981
                     /locus_tag="VF089_18105"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6175929.1"
                     /translation="MPSGLYPQHVTRNPLPKLLRSSGQAAVEFTLTFLILLIVAWIPA
                     DFGLAFYTGQLAQNAAREGARIAAADPTLVSGTASCVMPSCTGNIFKETAARLSSALL
                     PAATITLDLDPNTGTNCNRMVTVTVSGTYNFFFYQLLRWFGASGNLSDTPITRQTRMR
                     WEAQAGC"
     gene            2027..2545
                     /locus_tag="VF089_18110"
     CDS             2027..2545
                     /locus_tag="VF089_18110"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX6175930.1"
                     /translation="MNSAGENKAKRNNESGQSIVEIALITPLILIALYIPVDFGISFF
                     VANLTQTAAREGARVGSGLQKSGPSTNLVFSSAEANTVKAEVLSRLPSYLTNKTVTVT
                     FYRGTGCMEFLEVTAQGQYNFFMYQLLRLFGGDAPDSAIISRTTQMHYKYQPYMNNDY
                     CTNATTFGPYSA"
     gene            2559..3578
                     /locus_tag="VF089_18115"
     CDS             2559..3578
                     /locus_tag="VF089_18115"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6175931.1"
                     /translation="MQTVENERGYALIFVTVMIVLLLIMVGMALDSGHLAYVRSQGQP
                     AVDAAALAAASAIPSGKWSTVTDRAAKFNPGGSNPGTGNNYLDSPNNAIDQQNVTLVK
                     YDNATGEFTTSGVSIANANGARVALENNNPHGGSAGEAMKSPLFLTPLLNLFGQATEG
                     TANVSVSAVAVNQALPALPIVVVESSCGMDTTLDLRTGGKGNAGWETYQITNASTDEV
                     RGLFEKLPQCAGQPAIDVGYCGNIANGVNATVFNEHLKPMFKADPNRCYLIPVIKNGG
                     NLNQCQMITDWARFCPDKGNNTADAFPGQGLLKGRVTCGQSVHESRDSQCFVQRLVRD
                     KTSGM"
     gene            complement(3762..4019)
                     /locus_tag="VF089_18120"
     CDS             complement(3762..4019)
                     /locus_tag="VF089_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175932.1"
                     /translation="MSELLSLIRAIIVGFYFVMLVTPAFAAGKLKTPHAWWIFWAGLL
                     SGTMCLVFLSYLKMLVVFFAWIGLMIWAGIDVQIGRAKRID"
BASE COUNT         1054 a         1066 c         1059 g          981 t
ORIGIN      
        1 tttccaaacc accgaatatt gatcgcgctg catcaagttc gctgatcgaa aactggttga
       61 agttcgggtc tctggcctcg gtgccgggaa tgatccgaag tttcccagtg cgaatcgcgc
      121 tctgctcatt gaacgagcgc atccaatacg cgcaaatctc gtttctacca tttgaaagca
      181 ttgcgcctcg tagtgcttca agctcttgat cgtcaagcca ggcgagatcg aaaacgtcag
      241 gatcacctct ttggaatcgt tgtttcatga tctaaaacct cttgtccatg atgagtgacg
      301 gttcagacct cctgcagata agcgcgattc tgcttaacag ccccgtagag gtcaccgcac
      361 taacgacctt aaatctcctc cccaatcttt cttgcgaaac acgagatata cgctgctttc
      421 cctccacctc aagaactcta tgagaaggaa tttcgaagga agtcaggagg aggttcctac
      481 gacaaggtca gaaatccgcg ttgctgccgt acgtcttcag cctttgctgg ctgcggtttg
      541 gcgatctcat tggccatgct ggcaaagaag accgcaaaga agagcagaac attcacgact
      601 attagccctc tggcgacata gccccagata agatgccgga cattgaaggc gcatctccag
      661 acgccatata atgaccagag gaaagggatc agcaaaatga gaagcagctg ctcgcccgcg
      721 gtcccagcct catcagccag tttgatcaca aaggcaatcg ggaagaaaat aagccagtag
      781 gtcgtaaaca aactcgcctt gccggtcgag acctttgaaa tgatattcat gttgcctctc
      841 ctatgggagc attatcgttg ctgtcgctcg gttcattaga gcactgagca tgcccgtgac
      901 gattgttgat gtttgtctaa gggagctccc tctcttcatc aataccgccg acgagtaaac
      961 ccgatgagtg tgatggctct gagttagcag gcaaagttgc catcgcgcct ccagtgacag
     1021 aactcccgca tccgtccgaa tttctccaga gtattggctg tccgattgag agtgaggaca
     1081 caacgttgct ctcactccta cgagccagga cgaaggagcg cagcttgtaa ctacatcagg
     1141 atttaattga tgcgggcgcc gcattctcca tgatgcagtc aatttgggaa acaatcgttc
     1201 cgcccaactg tccccaagac tcctattgga tccaatcaat aacacgaagg ggtggtcgcc
     1261 agtagatatc ggcaaaggcg accaattttg ctcgtctcct gttcgttaaa tgcgaaaccg
     1321 atcgcgacgg tgacagcccg tgtcaatcga tgtgacggtt ttgacatcgg tttgtcatgg
     1381 tctggaacta gcagtgcatt ttgccaatcc tgcagcgctg cgtcattttg caattggaca
     1441 aacagcaatg ttggaagctg aagggaggag tcgctagatg ccatcaggtt tatacccgca
     1501 gcatgttacg cggaatcctt tgcctaagtt gcttcgttcc tccggccaag ctgctgttga
     1561 gtttaccctc acatttctaa ttctgctgat cgtggcctgg ataccggcag attttgggct
     1621 tgcgttctat accggacagc ttgctcaaaa cgcggcacgg gaaggcgcga gaattgccgc
     1681 agccgatccg acgctcgtaa gcggaacagc tagctgcgtg atgccttctt gtacggggaa
     1741 tatctttaag gaaacggcgg caaggctttc ctcagcgctc cttcccgccg ctacgatcac
     1801 cctggattta gatcctaata ccggcacaaa ctgtaatcgc atggtgacgg tcacggtatc
     1861 cgggacttac aattttttct tctatcagct cctacggtgg ttcggggctt caggaaatct
     1921 aagcgatacc ccaataaccc gtcaaacgcg tatgcggtgg gaggctcagg cggggtgtta
     1981 atctgtgttg ccatcgaagc ggcccttaca gataggaggc aaatcaatga actcagccgg
     2041 cgaaaacaaa gcgaagcgaa acaatgaaag cggtcaaagc atcgtcgaga tcgcgctcat
     2101 aactccgctt atactcattg cgctttacat acccgttgat ttcggtattt cgtttttcgt
     2161 agcaaatctt actcagacag cggctcggga gggagctcga gtcggtagcg gacttcaaaa
     2221 aagtgggccg agtacaaacc tagtattcag ttccgcagaa gcaaatacgg taaaagcaga
     2281 ggtactgagc cgattgccga gctatctaac aaacaaaacc gtgactgtga cattttacag
     2341 aggcacgggc tgtatggaat tcctcgaggt gacggcacaa ggccagtata actttttcat
     2401 gtatcagcta ttaaggttgt ttggcggtga cgccccagac tccgcaatca tctcgcgaac
     2461 cacgcagatg cactacaaat atcagcccta catgaataac gactattgta caaatgcgac
     2521 cacgttcgga ccatattctg cctaaacagg gaggtgcgat gcagactgta gagaatgaac
     2581 gaggttatgc gcttatcttc gtaaccgtga tgattgtgct gctcctgatc atggtcggca
     2641 tggcgttaga tagcgggcac ctcgcctacg tcagatccca gggacagccg gcagtggatg
     2701 cggcggctct ggccgcggct tcggcgatac caagcggaaa gtggtcaacc gttacggatc
     2761 gcgcggccaa gtttaatccg ggcgggagta atcccggtac cggcaacaat tatctagaca
     2821 gtcccaacaa tgcgattgat cagcaaaacg tgacgctggt gaagtatgac aacgccactg
     2881 gggaatttac cacctccggg gtcagtatcg ctaatgctaa cggagcgcgc gtcgccttag
     2941 aaaacaataa ccctcacggc ggctccgccg gagaagcgat gaagtcccca ctgttcttga
     3001 cgcctctgct caatctattt ggtcaagcca cagaagggac cgctaacgtg agcgtcagcg
     3061 cggttgctgt gaaccaggcc cttccagcac taccaatagt tgttgtggaa agttcttgcg
     3121 gtatggatac gacgcttgac ttacggaccg gtggcaaagg gaacgccggc tgggagactt
     3181 accaaataac taatgcaagc acagatgagg tccgcggtct tttcgagaag ttgccccagt
     3241 gtgccggcca gccggcaatc gatgtcggct actgtggaaa tatcgccaat ggcgtgaacg
     3301 caaccgtctt taatgagcac ctcaagccta tgttcaaagc tgaccctaac cggtgctact
     3361 taattccagt aatcaagaac ggcggcaact tgaaccagtg ccagatgatc acagattggg
     3421 cacgtttctg tcccgataag gggaataaca cagcagacgc gttccctggg cagggtttgc
     3481 tcaagggcag ggttacttgt ggccagagtg ttcacgagtc acgggatagc caatgtttcg
     3541 ttcagcgcct ggtgcgagac aaaacgtcag gtatgtaagg attcacggga gatcgttcgc
     3601 aacgagcttg gctagctgca gcaaaactca atgagaacgt ttttactcgt cggacttagt
     3661 gcgctttttt aaaatggctg tgctgctcct gtggctctga ctctttttgg ccgcgaaccg
     3721 agttaatcta tttgttaaaa agtttggctt cgagggcgtc attagtctat tcgcttcgcc
     3781 cttccaattt gaacgtcgat tccagcccag atcatcaagc cgatccacgc gaagaacacc
     3841 accaacatct tgaggtaaga aagaaacacc aggcacatgg tgccggacag aagtccggcc
     3901 caaaaaatcc accacgcgtg aggtgtcttg agctttccgg cagcaaatgc gggtgtcact
     3961 aacatgacga aatagaaacc gacgatgatt gcacgaatca aagataatag ctcactcatg
     4021 atttcctccg atgctaacgg tttttagctg cctcgatctg ataggaaagc tgttgatatt
     4081 tcttgagcag gcaagcttta actgaacaca gcgaaattcc cgtgatagtt taattccgta
     4141 ttcaatgtcc ggtaccgctt
//
LOCUS       DASUTG010000783         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_119854, whole genome shotgun sequence.
ACCESSION   DASUTG010000783 DASUTG010000000
VERSION     DASUTG010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119854"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="VF089_18125"
     CDS             complement(<1..1597)
                     /locus_tag="VF089_18125"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX6175933.1"
                     /translation="MSKPSIRIRGARQNNLNNLDLHIPLNALTVVTGVSGSGKSTLAF
                     DILYAEGQRRYVESFSAYARQFLDRMDKPDVESIEGIPPTIAIDQSRPVRTSRSTVGT
                     MTELHDHFKLLFAKIGVLHCRGCGRIVERDSSLSIVNKLSSLPAGSAIVLTFSLPRSS
                     VGWDEVRNGLQQAGFHRLLVDSAIEDLEAIDTAPSGKLLEVVVDRFAFRLENKKRICD
                     SLEQAFHYGKGKLNVFLPAEDWRREPFSNHLHCPHCDISYPEAVPNLFSFNSPLGACE
                     TCRGFGRVIDIDFDLVIPNPEKSLSDGAIKPWNSKPRRLRRLLDFCERKRIPVKKPYG
                     ELSQEHKALIVGGDGAFKGIRGWFRRLEQKSYRMHVRVLLSRYRCYLLCPDCQGSRVK
                     PGALNYLIDGKNIAEVSALSVGAAHRFFNQLKPAGSLDQVAALILEEIRRRLGYLDSV
                     GLEYLTLDRQSRTLSGGELERVDLTTAIGSSLVNTLYVLDEPSIGLHPRDSKRLVEIL
                     HRLKANQNTVVVVEHDPEIIKECD"
     gene            complement(1606..1848)
                     /locus_tag="VF089_18130"
     CDS             complement(1606..1848)
                     /locus_tag="VF089_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175934.1"
                     /translation="MKIDKEALLAEERRIRRLRRAMDIAVALLWQVDLTLAEAQDVVE
                     HAKKTALQLFPDKEETFDIIYGSRFRRVLVEKYRLQ"
     gene            2020..>2294
                     /locus_tag="VF089_18135"
     CDS             2020..>2294
                     /locus_tag="VF089_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX6175935.1"
                     /translation="MRISEIFYSIQGEGRLSGVPSVFIRTSGCNLRCVWCDTPYTSWS
                     PDGKEMSLDEILHAVESYPLSHVVLTGGEPLLSHEIEELSVTLKTAGA"
BASE COUNT          513 a          618 c          650 g          513 t
ORIGIN      
        1 ggtcgcactc tttgatgatt tcgggatcat gctcgacgac gaccactgta ttttgattgg
       61 ccttgagccg atgaaggatt tctaccaacc gcttgctgtc gcgcggatgc agcccgatcg
      121 acggctcgtc gagaacatag agcgtattga ccagagatga accgatcgcg gtggtgagat
      181 caacccgctc gagctcgccg ccggagagtg tgcgcgactg gcgatcgaga gttaaatatt
      241 ccaggccgac gctatccaga tagcccaggc gccggcggat ttcttccaag atgagcgccg
      301 ccacctgatc caacgaacct gccggcttga gctgattgaa aaatcgatgc gccgcgccga
      361 cgctcagcgc gcttacctca gcgatgtttt taccgtcgat caggtaatta agcgcgccgg
      421 gtttgacccg gctgccctga caatcgggac aaagcagata acagcggtag cgcgacagca
      481 acacgcgcac atgcatccgg tagctttttt gctccagccg gcgaaaccag ccgcgaatcc
      541 ccttgaatgc gccgtcgccg ccgacaatca acgccttgtg ctcttggctg agctcaccgt
      601 agggtttttt gaccgggatg cgtttgcgct cacaaaaatc cagcagacga cggaggcggc
      661 ggggtttgct gttccacggt ttgatggcgc catcgctgag cgatttttcc ggattcggaa
      721 tcaccagatc gaaatcaata tcgataaccc ttccaaaacc gcggcaggtt tcacaggctc
      781 ccaacgggct gttgaaggaa aacaagttcg gcaccgcttc ggggtacgag atatcgcaat
      841 gcgggcaatg tagatgattg ctaaacggtt cgcgccgcca gtcttcggcg ggcaggaaca
      901 catttagttt tcccttgccg taatgaaacg cttgttcgag agagtcgcag atgcgcttct
      961 tgttttccaa ccggaaagcg aatcggtcga cgacgacttc gagcagtttg ccggagggcg
     1021 ctgtatcgat ggcttccaaa tcctcgattg cgctgtccac cagcagccgg tgaaaaccgg
     1081 cttgttgtag cccgttgcgc acctcgtccc atccgacgga agagcgcggc agagaaaacg
     1141 tcagaacgat ggccgaacct gccggcaggc tgctgagctt gttcacgatc gataaggagg
     1201 agtcacgttc gacgatccgc ccgcagccgc ggcaatgtaa cacgccgatt ttggcaaata
     1261 gcaacttgaa gtggtcgtgc agctcggtca tggtgccgac cgtcgagcgc gacgttcgca
     1321 ccggacggct ctgatcgatg gctatcgtcg gcggaatgcc ttcaatgctc tcgacatccg
     1381 gtttgtccat acgatcgaga aattggcgcg cgtaggcgga aaagctctcg acgtagcggc
     1441 gctggccttc ggcataaaga atatcgaagg ccagggtcga ctttcctgaa ccgctaacgc
     1501 cggtcactac agtcagcgcg ttgagaggaa tgtgtaaatc gaggttattg agattgttct
     1561 gccttgcgcc gcggatccgt atggaaggtt tgctcatatt ttgacttact gaaggcggta
     1621 cttctccacc aggacccggc gaaagcgcga gccatagata atatcgaaag tttcttcttt
     1681 atcaggaaat aactgcaacg cggttttttt tgcgtgctcg accacgtctt gagcttcggc
     1741 cagggttaga tccacctgcc acagcaacgc cacggcaatg tccatggccc gtcgcagcct
     1801 gcgaattctt cgttcttcag cgagcagagc ttctttatct attttcattg ggagggagag
     1861 gatcgagcga acttacagtt gagaacgact ttcatgcgcc cgaacctaat acacttaaca
     1921 taccgcgatg gtgagtcaag aaagtgacag gatcaagcgt cgacaactgt gtgtacctct
     1981 taactttcgg cgcgcggttt cctttgctag ggtggcttta tgcgcatctc agagattttc
     2041 tactcgattc agggagaagg acggctaagc ggagtgccgt cggtttttat acgcacgtca
     2101 ggatgcaatc tgcgttgcgt ctggtgcgac acgccctaca cctcctggag tcccgacgga
     2161 aaggagatga gtctggacga gatccttcat gcggtcgaga gttacccgct gagtcatgtg
     2221 gtgctcaccg ggggagagcc actactgtcc catgaaatag aagagctgtc ggtaacattg
     2281 aaaacagccg gagc
//
LOCUS       DASUTG010000784         4532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1155042, whole genome shotgun sequence.
ACCESSION   DASUTG010000784 DASUTG010000000
VERSION     DASUTG010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155042"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..352)
                     /locus_tag="VF089_18140"
     CDS             complement(176..352)
                     /locus_tag="VF089_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175936.1"
                     /translation="MAAHAGEKAQEDGIFHCQKCNEKVRVNKGATTPKCRTAVALPMM
                     SVLKKQAAAKQIVP"
     gene            822..1805
                     /locus_tag="VF089_18145"
     CDS             822..1805
                     /locus_tag="VF089_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175937.1"
                     /translation="MKSMILVRVLLISSLLGFGFTINGQTAERKLINIGWTGGSNWTS
                     LPYQVAADRKFFEKEGLNVRLITMRGTTLMLSALMADEIDYVTILPFIAGAAVRGLPV
                     KIVASAAKSSGYAIISSPEITSLKELKGKRIAINSFGSSADFAIYTALRRSGLDPDKD
                     VTLQTVSGTPEARFAALISRAVDATVVGSPFEYFAEQKGFKTLVSVSEMAELVKIPIT
                     GISTSQRKIDKQPDEIVRVLRALRAATLFIQSEREIGIGLMENTLKLDRSAAERFYAL
                     YREQYNPELSVPNSVVEEWIAVGTFRAKDKEKGGAQGQRVHDWTFAEKAKQ"
     gene            1894..2802
                     /locus_tag="VF089_18150"
     CDS             1894..2802
                     /locus_tag="VF089_18150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6175938.1"
                     /translation="MQTDRTRIYEWNRHPRMPVPPPPPGSCDCQFHIYEEPGKFPPRA
                     NPPYPPIESATFSAAQNMHQAIGFDRGVIVHSAIYGSDHRLLVHALAGLEDRGHYRGI
                     GIIDDSVSDNELARLHAAGVRGARFNFVRFLTLDQREAEVRRSMARIRELGWHARLHV
                     HGDDLLNNSDLLRSMKDLPMVIDHFGHVGFEGGLERPVIRWVLDMLKHENWWMMVSNG
                     NRDSKMDASWDDAIPYGKAFIEAAPDRIVWGTDWPHPQWSKRMMNDAEEVELLYRYVD
                     GDPALLKKILVDNPARLHGFDKILSE"
     gene            3115..4176
                     /locus_tag="VF089_18155"
     CDS             3115..4176
                     /locus_tag="VF089_18155"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX6175939.1"
                     /translation="MAETNPNYSTPSSVRSVTSYPARRGFKQGPVDPSLDYVIGVEDS
                     IDIHCHAHEGQQDALGVAKLASKSGMKGILYKTIVGRKDPAGTVAGVRTALESWCAEK
                     GFTPVHVWPGSSVTQGFLSKIEPAWSAKMLDAGVVGLWMPNNTSANTLSIVGGKPSAW
                     DKTADPKAHTEPLPWSEALKYGHYLLDDKGRLLPEIEEIFRMAQDRGAAIFFGHPTKP
                     EFKAMAEFSQKIGFKKGIVDHPFSPFVNLTIEEMKDAPSAGLWLNFTFDELSPLLGID
                     PANMYKAIRTIGPEHCTLSSDAGEPLFPNSVESLRLLRGYMSAFGCSAEEIYTMCTVN
                     PAFIVGLDLAATQRASAAV"
BASE COUNT         1050 a         1218 c         1219 g         1045 t
ORIGIN      
        1 caatcgttca tgccctcggt acgaactttc accgaaatca ggcttgggtt gcatctccga
       61 ttcgacaccc ggccactctt gacgttgctc cgcaaacggt ggctgcggcc cttgctctcg
      121 tggatcgggc atttatttaa gcctccttcg agctaacaag ccgtcgcccg ctattttaag
      181 gtacaatttg cttagcggcc gcttgtttct tcagtacgct catcataggt agcgccaccg
      241 cagttcggca tttcggagtc gttgcccctt tgttgacgcg aaccttttcg ttgcattttt
      301 ggcagtgaaa tattccgtct tcctgagcct tctcacctgc atgtgctgcc atgctcaatc
      361 ctctttgcgg gtgaatctat tattgctcca tcctctttga ggaatgtgcc gagcttgaac
      421 gttaacgaac tgcttggatg tttgtgagta tgcgatgcaa gttctcttcc atcagtattg
      481 cctatgcact gcttcaactc tgtttcattt gggcgacaat accgcgggtc taaatcccgc
      541 cgtaagaatg ccgcaaggag atcgagctga atccatggtt tgagcttcat aaaaacccgc
      601 cttcaccacc cgagagggct gatgaataag cccgaagacg ccgatgttct tcattgactg
      661 cgaaatggct tctctcctat aactttgtag cagacagttt cactcgtgcg tggatccaac
      721 ggtgatacga gttctctgtt gatatcgttc ggcttggaat ttcgatcaac gacttggatt
      781 taaccactgc tgctgttgag gattcgacgg aggaaaaccc aatgaaatct atgattctcg
      841 ttcgtgtact cctcatctca tctctcctgg gttttggatt tactatcaat gggcaaaccg
      901 cagaaaggaa actgatcaat atcggttgga ccggtgggtc taactggacc tcgcttccgt
      961 atcaggtggc ggccgacaga aagttctttg aaaaggaagg gctcaatgtc aggctgatta
     1021 ccatgcgcgg aacgaccctg atgctcagcg ctcttatggc ggatgagatc gattatgtca
     1081 cgatcctgcc ttttattgcc ggcgctgcag tccgcggact tcccgtgaaa atagttgcat
     1141 cggcggcgaa atcgagcggc tatgcgatta tctccagccc ggagattact agccttaaag
     1201 agctcaaagg caaaagaatc gcgatcaact cgttcggcag ttcggccgat ttcgccattt
     1261 atacggcgtt aagacgaagt ggcttggatc ccgataaaga tgtgacgttg cagaccgtca
     1321 gcggaactcc cgaggcccgc tttgcagcat tgattagccg cgccgtcgat gccacagttg
     1381 tcggcagtcc gtttgaatac tttgctgagc agaaaggatt taagacgctc gtctcggtta
     1441 gcgagatggc cgagctcgta aaaataccga ttaccggaat cagcacctcg cagcggaaaa
     1501 ttgataagca gccggatgaa attgtccggg tgctaagagc gttaagggcc gccactttat
     1561 ttatccagag cgagcgcgag atcggcatcg ggctaatgga gaacaccttg aagttagacc
     1621 gttccgcggc ggaacgattc tatgctctct atcgcgagca atataatccc gagctcagcg
     1681 tgccgaactc cgttgtagaa gaatggattg ccgtcggaac ttttcgcgcc aaggataaag
     1741 agaaaggtgg agcccagggt cagcgggttc atgattggac gtttgcggaa aaagccaagc
     1801 agtaattcgc tcaacaaaat cgttccaggt ttatgggttc gaaagttcaa atcagatctg
     1861 gcaactaatc tcagacggca ggacggcgtc acgatgcaaa cagaccgaac gaggatctac
     1921 gaatggaaca gacacccgcg catgccggtt ccgccgccgc cacccggaag ctgtgattgc
     1981 cagttccata tttacgaaga gccggggaag tttccaccgc gagccaatcc gccgtatcca
     2041 ccgattgaaa gcgccacctt ctccgcagca cagaacatgc accaggcgat cggcttcgac
     2101 cgcggcgtca ttgttcatag cgcaatttac ggcagcgacc accgtctact agtgcacgca
     2161 cttgcaggcc tggaggatcg aggccactat cgcggcatcg gtattattga cgacagcgtt
     2221 tcagacaacg agcttgcgcg gctgcacgcc gccggtgtgc gtggcgcgcg gtttaatttc
     2281 gtgcgatttc ttaccctcga tcaacgggag gccgaagtca ggcgctcgat ggcccggatc
     2341 cgcgagctcg gatggcacgc gcgactgcac gtgcacggcg atgacctgct caataattcc
     2401 gatttgttac gcagcatgaa ggacctgcca atggtgatcg atcatttcgg ccatgtcgga
     2461 ttcgagggcg ggctcgaacg acctgtgatc cgttgggtac tcgacatgct caagcatgag
     2521 aattggtgga tgatggtttc caacggcaat cgcgattcga agatggacgc cagctgggac
     2581 gacgccatcc cctatggcaa ggcatttatc gaggcggcgc ctgaccgcat cgtctggggt
     2641 accgactggc cgcatccgca gtggagcaag cgcatgatga atgacgcgga ggaagtcgag
     2701 ctgctctacc gttacgttga cggagatccg gcattgttga aaaagatcct ggtcgacaat
     2761 cctgccaggc tacatggatt cgacaaaata ttgtcggagt gacgatcacg atcgatgaat
     2821 aacccgcagg aagccacggt ttatcaaaag tcggtaatca gattgtttga aagcatcacc
     2881 ccgaatgttt ttatcgggag tccaatgttc aatccgcctg gattcccgca gagcatgcgg
     2941 gaatgacgta cctcgaatag acaatttcaa gggaagaact tgcgcggagc aaaccacaaa
     3001 gcttgaacgg caaagcgcac acgtggaagg cgtccacatg cggatgagaa aacgtttgcc
     3061 ggacacatta cgaagcgata ttgtccaatc aatttgaaga accggaggac tgccatggcc
     3121 gaaaccaatc ctaattactc caccccaagc tcagttcgct cagtaacctc gtatccggcc
     3181 cggcgcggct tcaagcaagg tccggtggat ccgtcgctcg attatgtgat cggcgtcgag
     3241 gactctatcg acattcactg tcacgctcac gaaggccagc aggatgctct cggagtcgcc
     3301 aagctcgcct cgaaaagcgg catgaaagga atcctatata aaaccatcgt gggacggaag
     3361 gatccggcgg gtacagtggc cggcgtgcgt accgctctgg agtcctggtg cgccgagaag
     3421 ggttttactc ccgtgcatgt ctggcccggc agctcggtca cgcaaggatt tctttcaaag
     3481 atagagccgg catggtcggc caagatgctc gacgcaggcg tcgtcggcct atggatgccg
     3541 aacaatactt cggccaacac gctctccatc gtcggcggca agcccagcgc atgggacaag
     3601 acggcagatc cgaaagctca taccgaaccg cttccctggt ccgaggcgtt gaaatacggc
     3661 cactaccttc tcgatgacaa ggggcgcctt ttgcccgaga tcgaagagat cttccgcatg
     3721 gctcaagatc gcggcgccgc aatcttcttt ggccatccga ccaagccaga gttcaaagcc
     3781 atggcggagt tcagtcaaaa gatcggcttc aaaaaaggca tcgtcgacca tcccttcagc
     3841 ccgtttgtca atttgaccat cgaggaaatg aaagatgcgc cctccgccgg cctgtggctc
     3901 aacttcacct tcgatgagtt gtcaccgctc ctcggcatcg acccggcaaa catgtacaaa
     3961 gcgattcgaa ccatcggtcc cgagcactgc acgctctcgt cggacgccgg cgagccgctg
     4021 ttccccaact cggtcgaatc gctgcgcctg ttgcgcggct acatgtcggc attcggctgc
     4081 agcgcggagg agatctacac gatgtgtact gtgaacccgg cgtttattgt gggactcgat
     4141 ctggctgcga cgcaacgggc gagcgcggcg gtgtaagcag gttgccggca cactgacatg
     4201 cacggactac gacaacatca tgttgggtta gtctatctgt ccccgcggtc gaacatgatc
     4261 ctgaatacgt aagcggcacc tggctttctt gcggcatacc tctgccgatc tcttcttttg
     4321 acgggaacca tcgatcaatt tgtcgatcgg ttccaggtga cgtgccagcc gtaatcaatg
     4381 agactatagc aaactgaggg cggtgcttat ccactcggta ttcagctggt cagtatatgc
     4441 tagtcgatga aatcatactc gcttttcctt tgctgacttc ggttctcggc gaaaatgggt
     4501 gcgtcagaat ctttggtatg aagaattcat at
//
LOCUS       DASUTG010000785         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1155310, whole genome shotgun sequence.
ACCESSION   DASUTG010000785 DASUTG010000000
VERSION     DASUTG010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155310"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="rplL"
                     /locus_tag="VF089_18160"
     CDS             <1..229
                     /gene="rplL"
                     /locus_tag="VF089_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6175940.1"
                     /translation="AAAEEKTEFDVVLSEAGEKKIQVIKVVRELTGLGLKEAKDLVDG
                     APKNVKEGIAKAEAETIKKKLEEQGAKVDIK"
     gene            298..>3081
                     /gene="rpoB"
                     /locus_tag="VF089_18165"
     CDS             298..>3081
                     /gene="rpoB"
                     /locus_tag="VF089_18165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6175941.1"
                     /translation="MPFQVANNLRFRRSFGRIKRIIDLPYLIEIQKNSYELFLQKDSS
                     PAQRENIGLQEVFKSVFPIKDFNETASLEFVSYSLGEPKYDVDECHQRGMTYAAPLKV
                     TVQLVLWDVDAQTGARSIKNVKEQEVYFGEIALMTKNGTFMVNGTERVIVSQLHRSPG
                     VFFEHDKGKTHASGKLLYSARIIPYRGSWVDLEFDPRDVVYVRIDRRRKFHATVLLRA
                     LGMTTEDLLNYYYRSDTIVFDGRKPALIFNPAHLMGAKVTSDVRDPKSNDLIVKEGKK
                     FTRPVLRQMEQAKVKQVPIAWEEILGRIASHDIVNPQTKEVIVESNHPITQEKLDLIR
                     EKGIHQIEVLFIDESTVGPYLRNTLLQDRINSPQDAILEIYRRLRPGDPPTVDSATNF
                     FNNLFFNPDRYDLSKVGRLKLNHRLKLNVPLEQGTLRKEDILEVVRYLMELKNGNGTV
                     DDIDHLGNRRVRAVGELVENHFRVGLVRMERAIKEKMSLQDIETLMPQELINYKPVSA
                     ALKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRV
                     CPIETPEGPNIGLIASLTTYARVNEFGFIETPYREVENGKVTDRIRYLSALEEEDHVI
                     AQANAPIDARGVFTLDLVSARKGGEFVMARPEEVNFMDVSPNQLVSVAASLIPFLEND
                     DANRALMGSNMQRQAVPLLRTEAPLVGTGMERVVARDSGVTVVAKRDGVIESVDSTRI
                     VVKADKASGARDTGVDIYNLVKYQRSNQNTCVNQRPIVRIGDQVKAGDVIADGPSTEM
                     GELALGRNVLVALMPWGGYNFEDSILISERVVKEDIYTSIHIEEFECVSRDTKLGPEE
                     ITRDIPNVGDEALTDLDDSGIIRIGAEVKPGDILVGKITPKGETQLSPEEKLLRAIFG
                     EKAGEVRDTSLRVP"
BASE COUNT          783 a          742 c          842 g          714 t
ORIGIN      
        1 ggcagcagcg gaggaaaaaa ctgagtttga tgtcgttctt tcagaagccg gcgaaaagaa
       61 aatccaggtt atcaaggttg tccgcgagct caccggtctt ggtttgaaag aggcgaagga
      121 tctcgttgat ggcgcgccga agaatgtcaa agaagggata gccaaagccg aggctgaaac
      181 gatcaagaaa aagcttgaag aacagggagc taaggtcgac atcaaatagc gcaccagcac
      241 cacctgggac tttgttcgtg acacggtact actgtttaga tcgctaaagg agagacaatg
      301 ccatttcaag tggccaataa tttacggttt cgccgcagtt ttggcaggat caagagaatc
      361 attgatctgc cttatctaat tgaaatccag aagaactcct atgagttgtt tttgcagaag
      421 gattcttctc cggcgcagcg cgagaatatc ggccttcaag aggttttcaa atcggtattt
      481 ccaatcaagg atttcaatga gacagcgtcc ctggaatttg tcagctatag cttgggagag
      541 ccgaaatacg acgtagacga gtgtcaccag cgtggaatga cgtacgccgc gccgctcaag
      601 gttaccgttc agctcgttct atgggatgtc gatgcccaga ccggcgctcg cagcatcaag
      661 aatgtcaaag agcaggaggt ctatttcggt gagattgctc tcatgacgaa gaacggcacg
      721 ttcatggtga acggcaccga acgggttatt gtcagtcaat tgcaccgatc gcctggagtt
      781 tttttcgaac acgacaaggg aaagacgcac gcgagcggca aattgctcta ttcagcgcga
      841 attattccgt accgaggttc ctgggtcgat ctcgagtttg atccccgaga tgtcgtttat
      901 gtccgcatcg atcgccgtcg taagtttcac gccacggtcc tgctgagggc gctcgggatg
      961 actacggaag atttgctgaa ttattattac cgcagcgata cgatagtttt cgatggccgc
     1021 aaaccggcgc tcattttcaa tccggcgcat ttgatgggag cgaaagtgac aagcgatgtt
     1081 cgcgacccga aaagcaatga cctcatcgtt aaggaaggta aaaagtttac ccgcccggta
     1141 ttgcgtcaaa tggagcaagc caaggtgaaa caggttccta tcgcctggga agagattctg
     1201 ggtcggattg cttcgcatga catcgtcaat ccgcagacca aagaagtgat cgtggaatcc
     1261 aatcatccca ttacccaaga gaagttggat ctcatccggg aaaagggcat tcatcagatc
     1321 gaagttctct ttatagacga atcgacagtg ggtccttact tgcgaaatac gttgttgcag
     1381 gatcgcatca actcacctca agatgcaatt ttagagattt atagacgctt aaggccaggt
     1441 gatcctccga cggttgattc ggcgacgaac ttcttcaaca acttgttctt caatcctgat
     1501 cgctatgatc tttccaaagt aggtcgactc aagctgaatc atcggcttaa gctcaacgtc
     1561 ccattggagc aaggcacgct tcgcaaggag gatattcttg aggtcgtccg ctatttgatg
     1621 gagctaaaaa acggcaacgg caccgtagac gacattgatc atctcggcaa ccgtcgagta
     1681 cgggccgtgg gcgagctcgt agagaaccac ttccgtgtcg ggctggtgcg tatggaacgg
     1741 gccatcaaag aaaaaatgag cttgcaggat atcgaaactt tgatgccgca ggagctgatt
     1801 aactacaagc cggtttccgc ggcacttaaa gagttttttg gttccagcca actgtcccag
     1861 ttcatggatc aaactaatcc actttcggag attactcaca aacgccggct atccgcactg
     1921 gggcctggcg gactgacccg tgaacgcgca ggattcgagg tacgcgatgt tcatccgaca
     1981 cactatggtc gcgtttgccc gatcgagaca ccggaaggtc ctaatattgg gctgatcgcc
     2041 tccctgacga cgtatgctcg agtcaatgag ttcggcttca tcgaaacgcc gtatcgcgag
     2101 gttgaaaacg gcaaggtcac ggatcggatt cgctatcttt ccgcacttga agaggaagac
     2161 catgtcatag cgcaagcgaa tgcgccgatt gatgcccgtg gagtctttac tctcgactta
     2221 gtctccgctc ggaaaggcgg cgaattcgtg atggcgcggc ctgaggaagt taactttatg
     2281 gacgtgtcgc caaaccaact ggtcagcgtg gcggcctccc tgatcccatt tcttgaaaac
     2341 gatgacgcta accgcgctct gatgggatct aatatgcagc gtcaggcggt gcctctgcta
     2401 agaactgagg ctcctctcgt tggtaccggg atggaacgag tcgtggctcg ggattcaggg
     2461 gtcactgttg tcgcgaaacg cgacggcgta atcgagagtg ttgattccac tcgcattgtg
     2521 gttaaggctg acaaggcttc cggtgcccgg gataccggag ttgatatcta caatctagtc
     2581 aaataccagc gttcgaatca gaatacttgc gtcaatcaac gaccgatcgt caggattggt
     2641 gaccaggtaa aagccgggga cgttatagcc gacgggccat ccacagaaat gggagagctt
     2701 gcgctgggcc gcaatgttct tgtcgcgtta atgccctggg gcggctacaa ctttgaagat
     2761 tctattttga ttagcgagcg tgtcgtcaaa gaagacattt acacatcgat tcatatcgaa
     2821 gaattcgagt gcgtttcgcg cgacaccaaa ctcgggccgg aggagattac gcgcgatatc
     2881 ccgaacgtcg gcgacgaagc tctgaccgat cttgacgaca gcggtatcat cagaatcggc
     2941 gccgaggtca agcccggtga tatacttgtc ggaaaaatca ctccgaaggg cgagacgcag
     3001 ttgtctccgg aagagaagct tctgcgtgcc atcttcggcg aaaaggccgg tgaagttcgt
     3061 gacacatcgc tgcgtgttcc g
//
LOCUS       DASUTG010000786         6773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_860628, whole genome shotgun sequence.
ACCESSION   DASUTG010000786 DASUTG010000000
VERSION     DASUTG010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860628"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1172)
                     /locus_tag="VF089_18170"
     CDS             complement(162..1172)
                     /locus_tag="VF089_18170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175942.1"
                     /translation="MARDIFPEVKMHRLLRIGLLVPVLAVSLVWPATRASAAELRKFV
                     MGYSTVGAMAAGMWMAKEIGAYEKYGIDAELIYISSGPTVVQALIGGDVVGGVAATNA
                     VIAAVLRGAPLVSVLSTCNRPYHRLWVQPEIKTFDDLKGKVLGVTRFGSVTDNLTRMV
                     LKKYGLENSVQVRQMGGTLEVGAAFQNKQIAGAVTSSLRVGEKIQPRLLAKLEDQGFQ
                     YSMDVIAHARDDLKKFPTVVEGMVRAYTEGVAAMHFQKERAHRAIAKYARSRDQKQID
                     EIYQDSIIYLEKTPRVEPESINSILEFMGKPGVPLETFADNSIVDKMVREGFIDRLYK
                     RP"
     gene            complement(1294..1452)
                     /locus_tag="VF089_18175"
     CDS             complement(1294..1452)
                     /locus_tag="VF089_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175943.1"
                     /translation="MIDSWTVVIERCELSKRVQPRHTGFSHSLTIKAHPRAYKPRATD
                     GGTIGAQF"
     gene            complement(1449..3695)
                     /locus_tag="VF089_18180"
     CDS             complement(1449..3695)
                     /locus_tag="VF089_18180"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6175944.1"
                     /translation="MKVERDFSIIGTSVQRVDGAEKVTGKARYAGDLVVPGMIEGKFL
                     RSPYAHARILSIDTRQAEALPGVVAVITSKDFTDISPYMGRGKNKDHPIIAVDRVIFA
                     GQPVAAVAAVDRATAEEALAAIAVEYQELPAVIDVEDALAAGAPRIHEFAEKNICFQT
                     ELIKGDVETGFAEADEILEDTFEFPMIYHYSMEPHIAIAEFDNDGINIWTSTGHPFGV
                     RQEIAEIYHYPLSKIRVHVNFVGGAYGSKSGGKIEPLVVALARKAQRPVRVALNVAEA
                     MATCRRHAIKCKLKTGVKRDGTLVAKEAEIYLNTGAYAETGPIVTGRTLTRILGPYRY
                     PNLKINSYCVYTNTVSAASFRSIGGPQTAWATESQMDMIAHKLGIDPVELRLKNLVRK
                     GEELRPKYRPLDTDLAKGFKLVTEKLGWDGPLSREGHGRGVGFGTTDPGAPLASTATV
                     HVLADGSVVFMCGTSELGQGAKTIMSQIIAEELRVPLDRVTIRPIDTAFTPFDRSTGS
                     SRSTTVMGKAIELAGSDARRQIVELAVEHFECPEDAITLRDGEVMAGGKKISYGELIN
                     RHFADQGGELVGTGYAHSGMAPTPANPLFWEIGIGAVELSVDRETGKVRVEKYVTAAD
                     AGKAIHPLQCEGQDEGSAMMGFGHTFYEAYEWDGGQIINSSLVDYKVPTFDDVPDKFQ
                     SILIEDGNGPGPYGAKGLGEGGIIPVAPAVANAVFWCTGARIKSLPLTPEKIWRAIRE
                     SCQQQETK"
     gene            3986..4972
                     /locus_tag="VF089_18185"
     CDS             3986..4972
                     /locus_tag="VF089_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX6175945.1"
                     /translation="MKRHSLLNYIGTSLLLVLFSGCSARAPSGIVQLRPFEIESKENR
                     RTEFDGLILFSAFELRSRHPQFGGISGLSIGGDGRLYAVSDRGYWLSAQMRHDSEGRL
                     VDLRDWEIKPLLTTARTPSDGLLTDAEALAKAPDGSLIVGFEQNHRLWRYLAPPMTFD
                     SPAQPITLPRDLAKAPRNGGLECVTVLPSGEIFTVAEELQNKDGSFKGWLIVDQETVE
                     FSYLSTAGFRASDCTALPNGDVIVLERHLSIFFGFSARLKLIRAASIRANAALNGEDL
                     LRLDPPLRLDNFEAIAAREDAAGTLIYLISDDNFQPFQRTLLLQFRLPRADH"
     gene            5030..6172
                     /locus_tag="VF089_18190"
     CDS             5030..6172
                     /locus_tag="VF089_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX6175946.1"
                     /translation="MSQIDQHCVLSMLDNQPTYIFAAEYLKNFSAAVFMHFGISAEDA
                     MQAADVLATSDLRGIDSHGVARLHTYFEMLELGRINPNPNIRIVREKHSVATIDGDNG
                     LGLVVAPKANAIAMDKAEQHGSGWVSVCNTNHFGIAGYYPLQALKRDLIGWAMTNTTK
                     LVAPLWGAERMLGTNPIAIAFPGQEEPPIVIDMATSAVAYGKIEIALRKKESVPKGWI
                     VDRDGRDTSNPQDMIDGGAQLPLGSEKELGGHKGYALAAMVDILCCVLSGANWGPFAP
                     PFALRQDIPDRSVGKGIGHFFGAMQIDGFIDKGQFKKQVDEWIRVFRNTKPAPGTNGP
                     LIPGDPEREAEAIRSKEGIPLIKPVVDDLLEISEKTGIPFEISRAT"
     gene            6210..6521
                     /locus_tag="VF089_18195"
     CDS             6210..6521
                     /locus_tag="VF089_18195"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEX6175947.1"
                     /translation="MVTAFVQFKLPSAMSREQAKEVFMSTAPKYQAAPGLIRKYYLLS
                     EDGGTAGGVYLWQSKGDAEHLYTQEWKNFVKEKYGSEPTIVYFHSPVIVDNLTKEIIK
                     D"
BASE COUNT         1485 a         2005 c         1766 g         1517 t
ORIGIN      
        1 ctgaaaagta atgctccagc tgcggcgatc gcatgtgatc gcgcaccatt tctccgatcg
       61 tatttcgcca ttcggcaacc tgcatatcaa atctccctta cgtgaggatc tcgtttgccc
      121 gggccgcctg tcttccctat atccgatcac cgcttagtta ctcaaggcct cttatacaat
      181 ctgtcgatga atccttcgcg caccatcttg tcgacgatgg aattatcggc gaaagtctcc
      241 agcggcacgc cgggtttgcc cataaactcg agaatagagt tgatcgactc cggctccacg
      301 cgcggcgttt tctccagata aataatcgag tcttgataga tctcgtcgat ctgcttctga
      361 tcgcgggagc gcgcatactt ggcgatcgct cgatgcgccc gttccttctg gaaatgcatt
      421 gcggcgacgc cttcagtata ggctcgcacc ataccttcca cgacggtggg aaacttcttc
      481 aaatcgtcgc gcgcatgagc gattacgtcc atcgaatact gaaacccctg atcctcgagc
      541 ttggccaata agcgcggttg aattttctca ccgacccgca agcttgaagt cacggctccg
      601 gcaatctgct tattctgaaa cgccgcgcca acctcaagcg tcccacccat ctgccgcact
      661 tgcacggaat tttccaagcc gtattttttg aggaccatgc gcgtcaaatt gtcggttacc
      721 gacccgaagc gcgtcacgcc gagaactttg cctttcaggt cgtcgaaggt cttgatctcc
      781 ggctgtaccc acaagcggtg atacggacga ttgcaggtgc ttaataccga caccagcggg
      841 gcaccacgca acactgctgc aatcactgcg ttcgttgccg caacgcctcc gaccacatca
      901 ccgccaatta acgcctgcac caccgtcggt cccgaggaga tgtagatcaa ctccgcgtcg
      961 atgccatact tttcatacgc gccgatctcc ttcgccatcc acatccccgc ggccatggct
     1021 ccgacggtgg aatagcccat gacgaatttc cgtaactcgg ccgccgaggc tcttgtggca
     1081 ggccatacca gggaaacagc gagcaccggg accaacagtc cgattcgaag caatctgtgc
     1141 atcttcacct ccggaaaaat atctctagcc atacgatgtt catccgatgg agtaactacc
     1201 atacccgcac acacactcac cgttacggcc gttggatcta gattaaaaag cttacctcaa
     1261 ggctgagctg catattcgcg gctcaaatgc cgcttaaaat tgagcaccga tggtgccgcc
     1321 gtccgtcgcc cgtggcttgt acgcgcgagg gtgtgccttg atcgtgagtg agtgcgaaaa
     1381 tccggtgtgc cgaggttgga cgcgcttgga cagctcgcat ctctcgatga ccaccgtcca
     1441 gctgtcgatc atttcgtctc ctgttgttgg caactttctc gaatcgctcg ccaaattttc
     1501 tccggagtca atggcaatga ctttatccgg gcgccggtgc accagaacac cgcgttcgcc
     1561 accgccggcg caaccggaat aatcccgcct tcgcccaaac ccttggcgcc gtacgggcca
     1621 ggaccgttgc cgtcttcgat gagaatcgac tggaatttgt cgggcacatc gtcgaacgtc
     1681 ggcactttgt aatcgactag agaggaattg atgatctgtc caccatccca ttcgtaagcc
     1741 tcataaaacg tgtgaccgaa tcccatcatc gccgagcctt cgtcctgtcc ctcgcattgc
     1801 aacggatgga ttgctttgcc ggcgtccgca gcggtaacgt atttctcgac gcgaaccttg
     1861 ccggtctccc gatccaccga gagttccacc gcgccgatgc cgatctccca gaacagcggg
     1921 ttagccggcg ttggcgccat gccgctatga gcgtaaccag tgccgaccag ttcaccgccc
     1981 tgatcagcga agtgcctgtt gatcaattcc ccatagctga ttttcttgcc gccggccatg
     2041 acttcgccgt cgcgcagcgt aatcgcgtct tccggacatt cgaagtgctc gaccgcaagc
     2101 tcgacgatct gccgtcgcgc atcgctaccc gctaactcaa tcgctttacc catgacggtg
     2161 gtcgaacggc tcgatccggt ggagcggtca aacggagtga aggcggtgtc gatggggcgg
     2221 atcgtgaccc gatcgagcgg cacgcgcagt tcctccgcga tgatctggct catgatcgtt
     2281 ttcgcgccct gacccaactc cgaagtgccg cacatgaaaa ccacgctgcc gtcggcgagc
     2341 acatgcaccg ttgcggtcga cgccagcggc gcgcccggat cggtggtgcc gaaacctacg
     2401 ccgcgcccat gcccttctct agaaagcgga ccgtcccagc ctaatttctc ggtaaccagc
     2461 ttgaatcctt tggccaaatc ggtgtccaat ggccggtatt tcggcctcag ttcttcgcct
     2521 tttcggacca aattttttag ccgcaactcc actggatcta tcccgagctt atgcgcgatc
     2581 atatccatct gcgattccgt cgcccaggcg gtttgcggtc cgccgatgga ccggaacgag
     2641 gcggctgaaa cggtgttggt ataaacgcag tacgagttga ttttaaggtt cgggtaacgg
     2701 tatggcccga ggatgcgcgt caaagttcga ccggtcacga tcggaccggt ttcagcgtag
     2761 gcgccggtat tgagatagat ctccgcctct ttggcaacca gggtgccgtc tcttttcacg
     2821 ccggtcttga gcttgcactt gatggcgtga cggcggcaag tggccatcgc ttccgcgaca
     2881 ttgagtgcta ccctaaccgg ccgttgcgct ttgcgcgcca gcgcaaccac cagcggctcg
     2941 atctttcctc ccgacttgct gccataggcg ccgccgacaa agttcacatg aacgcggatc
     3001 ttcgataatg ggtaatgata tatttccgcg atttcctggc gcacgccgaa cgggtgacca
     3061 gtggacgtcc aaatattgat cccgtcatta tcgaactcgg cgatggcgat gtgcggctcc
     3121 attgaatagt gataaatcat cgggaactcg aaagtgtcct cgaggatctc gtcggcttcg
     3181 gcaaaaccgg tttccacatc gcctttgata agctcggtct ggaagcaaat atttttctcg
     3241 gcaaattcat gaattcgcgg cgcgccggct gctaacgcat cctccacgtc tataaccgcc
     3301 ggtagttcct ggtattcgac cgcgatcgcg gctaacgcct cttccgcggt ggctcgatcg
     3361 acggcggcaa cggccgccac cggctggccg gcaaagatta cccgatccac ggcaatgatc
     3421 ggatggtctt tgttttttcc gcgccccatg tacgggctaa tatcggtgaa gtctttgctg
     3481 gtgatcacag cgactactcc gggcaacgcc tctgcttgcc gtgtatcgat cgagaggatg
     3541 cgtgcatgcg cgtacgggct gcgtagaaat tttccttcga tcattccggg gacaacaaga
     3601 tcgccggcgt acctggcttt ccccgtaacc ttctcggcgc cgtcaacgcg ctgcaccgat
     3661 gtgccgatga tcgaaaaatc gcgctcaacc ttcaagttac aaccttcctt ttgccgacaa
     3721 tccatcagaa ctatgttcgc ggcgacatga agacgattca aacagtagcc gttgtcgaga
     3781 gacgagctgt cgatacatcc tcccgtatat gggcattaaa ttcctgtcaa gctctttggc
     3841 ggtgaccatc gtgtatccta acgtggatcg acaggccggc attgaaaccg ttcatctctc
     3901 cgattcagcg ttcagcttcc gtgcgaatcc ttgagcactc catctataga tgcgatgatg
     3961 tctgagacag ttcattgaac acttcgtgaa acgccactcg ttactcaact atatcggcac
     4021 aagtctcctg ctggttttat ttagcggatg cagcgcgcgg gcaccgtcag gtatagtcca
     4081 attgcgccct tttgaaattg agtcgaagga gaaccgacgg acagaattcg acgggttaat
     4141 actgttcagc gctttcgaac tccggtcgcg ccatccgcag ttcggcggca tatccggcct
     4201 gagcataggc ggcgacggta ggctttacgc cgtctccgat cgcggctatt ggctttctgc
     4261 tcagatgcgc cacgatagcg aaggccggct ggtagacctg cgcgattggg aaataaaacc
     4321 gctcctaacc acggcaagaa ctcccagcga tggactgctg accgacgcgg aagcgctcgc
     4381 caaggccccg gacgggtcac tcatcgtcgg ctttgaacag aaccatcgcc tgtggcgcta
     4441 tctggcgccg ccgatgacct tcgactcacc ggcgcagcca atcaccttac cacgcgatct
     4501 tgctaaagcg ccgcgcaatg gcggtcttga atgcgtcacc gttcttccca gcggagaaat
     4561 attcaccgtt gccgaagagt tgcaaaacaa agacgggagt ttcaaaggct ggctcattgt
     4621 tgaccaggaa acggtggagt tttcttactt gtcaaccgcc ggcttccgag cttcggattg
     4681 caccgcgttg ccaaacgggg acgtcatcgt gctcgagcgg cacctatcca tcttttttgg
     4741 cttttcggcg cgcctgaagc tcattcgagc tgccagcata cgagccaatg cggccctcaa
     4801 cggcgaagat ctgcttcggc tcgatccacc actgcggctc gacaactttg aggctatcgc
     4861 agcacgggaa gacgcagccg gaactttgat ctacctcatc tcggacgata attttcagcc
     4921 gttccagcgc acactgctgt tacaatttcg cctgcctcgc gccgatcact gacgattgct
     4981 tttagacctc tggtactgca ttgacactga cacgtcacgt agagtaagcg tgagccaaat
     5041 cgaccagcac tgtgtcctca gcatgctcga taaccaaccg acctatattt tcgccgcgga
     5101 gtatctgaag aacttctcgg ctgcggtgtt catgcacttc ggcatttcgg cagaagatgc
     5161 catgcaagcg gcggatgtgc tcgcaacaag cgatttacgc ggcatcgatt ctcacggcgt
     5221 cgcgcgcctg cacacctact tcgaaatgct cgagctgggc aggatcaatc ccaaccccaa
     5281 catccgaatt gtccgcgaaa agcatagtgt cgcaaccatc gatggcgaca acggcttggg
     5341 attggtggtc gcgcccaagg ccaacgccat cgccatggac aaagccgaac aacatggctc
     5401 gggctgggtg agcgtctgca ataccaatca tttcggcatc gccggctact atcctttaca
     5461 ggcgctcaag cgcgatctga ttggctgggc gatgaccaac acgaccaaac tcgtagcgcc
     5521 gctatggggc gcggagagaa tgctgggaac gaatccaatc gccatcgcct ttccgggaca
     5581 ggaagaaccg cccatcgtca tcgacatggc gaccagcgcc gtcgcttacg gcaaaatcga
     5641 aattgccttg cgcaaaaagg aatccgttcc gaaaggctgg atcgtcgacc gagacggccg
     5701 cgacacgagc aatccacaag acatgatcga tggcggcgcg caactgccac tcggctccga
     5761 aaaagaactg ggggggcata agggctatgc gctggcggca atggtggaca ttctctgctg
     5821 cgtcttgagc ggcgccaact ggggtccctt cgctcctccc tttgcgcttc gacaagatat
     5881 tcccgaccgt tcggtcggca aaggcatcgg ccatttcttc ggcgccatgc agatcgacgg
     5941 ctttatcgat aaaggtcaat tcaagaagca ggtcgatgaa tggatccgag ttttccgcaa
     6001 caccaaaccc gctcctggaa ccaatggccc actcatcccc ggcgatcccg agcgagaggc
     6061 cgaggcgatt cgcagcaaag aaggtattcc cttgataaaa ccggtggtgg acgatttact
     6121 ggagatttca gagaagaccg ggataccgtt tgagattagc cgggcaacat gattcggtag
     6181 atcagttaac cgaaaaatgg gggtcagcta tggtgacagc cttcgttcag ttcaagctac
     6241 cgtcagctat gagccgagag caagccaaag aggtttttat gagcacggct ccgaagtatc
     6301 aggccgcacc aggattgatt cgcaagtact atcttctatc cgaagatggc ggcaccgccg
     6361 gtggtgtgta cctgtggcaa tcaaaaggag atgccgagca tctttatacg caggaatgga
     6421 aaaatttcgt aaaagaaaaa tatggcagcg agccgaccat tgtctatttt cacagtccgg
     6481 ttatcgtcga caatctgact aaggagatta tcaaagatta gccgcttacg attcgggtcc
     6541 gatgcgcacg cgagagagct tattctcaga tctccggcgc agcagcctga gagttagctc
     6601 gcgctactca attaacttat tgataattac cgggcgtgac cgttggcagt cgcagctgaa
     6661 ctctgcgctg aaatgtgaac gaagcggagt aagtccatag atacgcacat ttatcttagg
     6721 acattgacaa tcatcgatcg cgccgtcatc aaagcaagtg gatgtcaatt cct
//
LOCUS       DASUTG010000787         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2196826, whole genome shotgun sequence.
ACCESSION   DASUTG010000787 DASUTG010000000
VERSION     DASUTG010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196826"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF089_18200"
     CDS             <1..465
                     /locus_tag="VF089_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6175948.1"
                     /translation="YFGIVGFFTRAVELKAKDLPQATWNMGDPFTNLQDGLHLASQLL
                     ERKPSVNQQMIVITDGQPTAYCRQGRLYCEWPLSFGGISQRAAEETLKEAERITRKGI
                     TINTFMLDDSPVLRGFVDDLTRINKGRAFYTRPDRLGEYLLVDYLSHQRKKV"
     gene            720..1328
                     /gene="lexA"
                     /locus_tag="VF089_18205"
     CDS             720..1328
                     /gene="lexA"
                     /locus_tag="VF089_18205"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6175949.1"
                     /translation="MQPALTPRQKAIYEFVLKTIREKGYAPSIQEIGSRFKIASTNGV
                     SDHLKALEKKGYIRRIGKRAIEVLSALGKPVLSATREIQVLGRVPAGKPFFSEENSEG
                     FLTIPMDMGAGKLFALQVKGDSMIDAGILDGDRVIVKQQGTAENGQIVCAIIDGEATL
                     KRFYQEDGVITLKPENEKYAPITVSEGEFRIAGRVVGLLRKF"
     gene            1617..2093
                     /locus_tag="VF089_18210"
     CDS             1617..2093
                     /locus_tag="VF089_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HEX6175950.1"
                     /translation="MTFGLVREAQQRGEPVGWVTSMASCFYPPDAAQSGTDLAALVVI
                     RIPAAEKIARAGEKLLRSGGFGLLVFDLGTADIPMPLQSRLTGLAHHHQTALVCLTAK
                     QRKAFSLGSLVSLRVHAERKRASENLFACMVNVLKDKRHGPTWTHEELRFGPAGLC"
     gene            2038..>3055
                     /locus_tag="VF089_18215"
     CDS             2038..>3055
                     /locus_tag="VF089_18215"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX6175951.1"
                     /translation="MDQLGPMKSSASDRLACVDLPAFALQLLLKRHPEWRAYPAAVVA
                     EDKPQGLILQINERARRCGVLPGLRYAAAFSLARGLRAGEVRPDEIHKAIVALTQRLM
                     RFTPEVEPSAPEPGVFWLNGKGLQRLYASPREWAGAIYQDIKTQGLQANLVVGFSRFG
                     TYAVAKEKSGITIFDDPTEERSAARKVPLDRLNIEAKFRDTLFKLGIQTVGGLLSLPS
                     AGLRERFGAQAHRLYRMAAGDLWTPLDPCVLEETVAQRYLLDDPESDVTRLLFLIKQL
                     LHPMLATLAARTQALSALWLGFLIEKSEWLKEQIRPAVPTLEAAQILDLVRLRLETLK
                     FPAGV"
BASE COUNT          674 a          813 c          854 g          714 t
ORIGIN      
        1 tacttcggca ttgtcgggtt ttttacgcgc gccgtcgaat taaaagccaa ggacctgccg
       61 caagcgacgt ggaacatggg cgatccgttt accaatcttc aggatggttt gcatttggca
      121 tcgcaacttt tggaacgaaa gcccagcgtc aatcagcaga tgatcgttat caccgacggc
      181 cagccgacgg cttattgccg tcaaggccgg ctttattgcg aatggccatt gagtttcggc
      241 ggcatcagtc aacgcgccgc ggaagagacc ttgaaagagg ccgagcgcat cacccgcaaa
      301 ggcattacca tcaacacctt catgttggat gacagccccg tactgcgcgg cttcgtcgac
      361 gacctcaccc ggatcaacaa gggccgcgcc ttttacactc gccccgatcg cctgggagaa
      421 tacctcctgg tcgattatct ctcgcatcag cgaaagaaag tctaacgcga agcgttagac
      481 tttctgttcc agccgttcca gtagttccaa tcgttccaac cgtttcgaca acttatcagt
      541 gacttgagta cttgagccta gaggtttgaa gttttgtcta atttaactgc gagccattcc
      601 ggcacgcctt ccgatcccga tcgcatgact ggaacgattg gaacgttttg aacggttgga
      661 acgcggcctc ttggccgctc ctagttgact actgaaaata tattcagtat atctatttta
      721 tgcagcccgc gttgacaccc cggcaaaagg cgatctacga attcgtgctc aagacgattc
      781 gggagaaggg ctatgctcct tcgatccagg agatcggcag ccgtttcaag atcgcgtcaa
      841 ccaatggggt ttccgatcat ctcaaagctc ttgaaaaaaa aggctacatc cgccgcatcg
      901 gtaagcgagc cattgaggtc ttgtcggcct tgggcaaacc cgttctaagc gccactcgcg
      961 agatccaggt tctcggcagg gttccggcag gaaaaccttt tttcagtgaa gaaaactcgg
     1021 aaggattttt aaccataccg atggacatgg gagccggaaa gcttttcgcg cttcaggtta
     1081 agggcgacag catgatcgat gcgggtattc tcgatggcga ccgagtgatc gtaaagcagc
     1141 agggaacagc cgaaaacggc caaatcgtct gcgccataat cgatggcgaa gcaacgttga
     1201 agcgatttta tcaagaagac ggcgtgatca cgctcaagcc agaaaacgaa aagtatgcgc
     1261 cgatcacggt ttctgaagga gaattccgga tcgccgggcg ggtggtcgga ttacttagga
     1321 agttctgaac cacggagaag cttgttgaag cagttcaacc cgttcaaatc gttcaagtag
     1381 tttgaaggct aaaagccgaa gatgttttat aagaataacg gtaattggtg ctgctaactt
     1441 gagtttgaga atcttagacg gtttgaacat tttgaacgat ttgaacggct tgaacgattg
     1501 ttatgtctct tgtccccgct caaaaattag caccagcgtt aaatcgttgg tcgctcaccg
     1561 aggtcgctgg acgattagtt gaaatttccg cttcggtctc ttcggcggcg ttgactatga
     1621 cgtttggctt ggtgcgggaa gcgcagcagc gtggcgagcc ggtgggctgg gttacttcga
     1681 tggccagttg cttttatcct cccgacgcgg ctcaaagcgg cactgatttg gccgcgctgg
     1741 tggtgatccg cataccggcg gcggaaaaga ttgcgcgcgc cggcgagaag ctgttgcgct
     1801 cgggcggctt tggcttattg gttttcgatc tcggcacggc cgatattccc atgccgttgc
     1861 agagccgctt gaccgggctc gcccatcatc accagacggc gctggtttgt ctcaccgcaa
     1921 aacaacgcaa ggcgttttct ctgggatcgc tggtgtcgct acgggtccat gccgaaagaa
     1981 aacgcgcttc ggaaaatctc tttgcctgca tggtcaatgt tctcaaagac aaacgtcatg
     2041 gaccaacttg gacccatgaa gagctccgct tcggaccggc tggcctgtgt tgatctgccg
     2101 gcgtttgcgc tgcagctgct gctaaagcgc catcccgagt ggcgcgctta cccggcggct
     2161 gtggtggccg aggacaaacc gcaaggattg attctgcaga tcaatgagag ggcccggcgc
     2221 tgcggcgtgc ttcccggatt gcgctatgcc gcggcttttt ctttggcgcg tgggttgcgc
     2281 gccggggaag ttcggccgga tgaaattcac aaagcgatcg ttgccttgac ccagcggctg
     2341 atgcgcttca cgcccgaggt cgagccgtcg gcaccggaac cgggagtgtt ctggttaaac
     2401 ggcaaaggtc tgcagcgcct ctatgcgtcg ccccgagaat gggcaggcgc aatttaccaa
     2461 gacattaaga cgcaaggatt acaggctaac ctggttgtcg gttttagccg gttcggcact
     2521 tatgcggtcg ctaaagaaaa atcagggatt acgattttcg acgatcccac cgaagagcgc
     2581 agcgcggcac gtaaagtgcc gttggatcgt ctgaacatcg aagctaagtt tcgcgatacg
     2641 cttttcaagc tcggcatcca aacggtaggc ggtttgcttt cgcttccatc ggcagggttg
     2701 cgcgaacgtt tcggtgctca ggctcatcgt ctctaccgga tggccgccgg cgatctctgg
     2761 actccgctcg atccatgcgt ccttgaagag accgttgcgc aaagatatct gctcgatgat
     2821 cctgaaagcg atgttacccg gctgctcttc ctcatcaaac agctacttca tcccatgctg
     2881 gcgacgcttg ctgcgcgcac ccaggcgctc agcgcgctgt ggcttggttt tctgatcgag
     2941 aagagcgaat ggctcaaaga gcaaattcgc cccgctgtgc ccaccctcga agcggctcag
     3001 atactcgatc tggtgcgcct gcgcttggaa acgttaaaat tccccgccgg cgtta
//
LOCUS       DASUTG010000788         3971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_564414, whole genome shotgun sequence.
ACCESSION   DASUTG010000788 DASUTG010000000
VERSION     DASUTG010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564414"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF089_18220"
     CDS             complement(<1..246)
                     /locus_tag="VF089_18220"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HEX6175952.1"
                     /translation="MIKFTAACLFVLMAGTPAWAQFDRVLKGLGEMSGVGGGLNDRKI
                     AAGLQEALRIGTENAVFQTGSTDGFLKNQAIKILMPRP"
     gene            complement(319..711)
                     /locus_tag="VF089_18225"
     CDS             complement(319..711)
                     /locus_tag="VF089_18225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6175953.1"
                     /translation="MFAPLVFTIAVALLSSCATLRSFTPGHDSFEEGMASFNQGQFDS
                     AIQHFRKSTLENPNSAQAYLYLGRSYISVGRWKSAIQPLRTAFRLSPHEARDEIMNLI
                     VDAVFAAATMDSRSGESGSSGDRYKDLL"
     gene            complement(768..1091)
                     /gene="grxD"
                     /locus_tag="VF089_18230"
     CDS             complement(768..1091)
                     /gene="grxD"
                     /locus_tag="VF089_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX6175954.1"
                     /translation="MAEDVLNEIDSKVKSNKVMLFMKGTPDFPQCGFSAHTVEILRSY
                     GVPFATADVLADAAVRDGIKRYSNWPTIPQVYIDGKFVGGCDIVHELHERGELEPMLK
                     SAIAK"
     gene            complement(1119..1373)
                     /locus_tag="VF089_18235"
     CDS             complement(1119..1373)
                     /locus_tag="VF089_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX6175955.1"
                     /translation="MITPDEIKSTLEQALPGSTIEMQDLTGGGDHWQVFIVSPAFEGK
                     GLIEQHQMVYAALKEEMGDQRIHALALKTFTPEQWAKLGS"
     gene            complement(1431..1790)
                     /locus_tag="VF089_18240"
     CDS             complement(1431..1790)
                     /locus_tag="VF089_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6175956.1"
                     /translation="MQLRHYVEFQRELAINYCKLVVVSVDPPEVNSAFRTGLGAEFPI
                     LSDHDRKVVQALDMTETSKSRGVTAIPYTFSLMPDLTVHNLYCGYWYVGRPTLDELRM
                     DLRAMMKKVRSDFDPQA"
     gene            complement(1803..1931)
                     /locus_tag="VF089_18245"
     CDS             complement(1803..1931)
                     /locus_tag="VF089_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6175957.1"
                     /translation="MNQTIGKQFPDFQMLDHDSQPVKLSQYAGKFPLILSFYRGYW"
     gene            complement(1984..2973)
                     /locus_tag="VF089_18250"
     CDS             complement(1984..2973)
                     /locus_tag="VF089_18250"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6175958.1"
                     /translation="MNAIRVAALVLCGLWAATLAQAQELKKIKIGYPAVSYNQIHIWV
                     AKEAGLFRKYGLDAEVVFFRGGGMATQALVAGDPPIVNIGTVVLAGLQGYDVVLIGSS
                     ESTYNYSVVARPATTKLEQLKGKRLGVSGFGSASHTAALILLKKFNLEPNKDVMLVVS
                     GPTPDRLAAMESNRIDATILTPSETPRARKQGFIEIFDMLDLGIEVQGNGFATSRTFI
                     RNQRETALSALKGYVESIHYIHKNREETKKIVGHYLRGADAEALEATYTAFVKSVAKR
                     PYPTLKGIQYLLDDLAVKNPQAKTAKPEQFVDLSLLQQLEKEGFFDEMAKRYP"
     gene            complement(3227..>3971)
                     /locus_tag="VF089_18255"
     CDS             complement(3227..>3971)
                     /locus_tag="VF089_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6175959.1"
                     /translation="STDRKKLMNNVALITGATRGIGRAIAAAYLREGARVFICARNRQ
                     EVAAAVRELREDTTDAQVDGCAGDVGKENDVRRIVDAAIERFQTVDTLVNNASLLGPR
                     VALVHYPFGQWEEVIRVNLHGVFLLSQQVVKRMMAQRHGSIINVSSGVGRVGRARWGA
                     YAVSKFGLEGLTQVMADEVKEFGIRVNSVNPGPTRTGMRAEAYPEEDPLTLPTPEQIT
                     PIFVHLAAAQSENVTGQMFEAKDWSERAH"
BASE COUNT          883 a         1131 c         1001 g          956 t
ORIGIN      
        1 cggcctcggc attagaattt tgattgcttg atttttgagg aagccgtccg tcgaacccgt
       61 ttggaacacg gcgttttcag tgccgatcct aagcgcctcc tgcaagcctg ctgcgatctt
      121 ccggtcattc aagccccctc ccacgccact catctcaccc aatcctttta gcacccggtc
      181 gaattgcgcc catgccgggg tacccgccat gaggacgaat aagcatgctg ctgtgaactt
      241 gatcatgact taacgctcct aggtgacgac cggaaggacg atttgcctga agtgagtcac
      301 gggggaatgc cagatatgct agagcaaatc tttgtagcga tccccagacg aaccgctttc
      361 tcctgatctg gagtccattg ttgccgcggc gaaaaccgca tcgacgatca gattcatgat
      421 ctcgtctctg gcctcatgcg gtgacagtct gaacgcggtg cgcagcggtt ggatagcgga
      481 cttccagcgg cccacgctga tgtacgatct gcctaaatag agataagctt gcgcggagtt
      541 aggattttct agagttgact tcctgaaatg ctgaatcgcg gaatcgaact ggccttggtt
      601 gaacgaagcc attccctctt cgaagctgtc gtgcccggga gtaaagcttc taagagttgc
      661 acaggaactc aatagcgcca ccgcgatggt gaagactaat ggcgcgaaca ctttactcct
      721 ttttgacaac gcgagttatt gtggcttggc tcgatgaact ttattgtcta tttggctata
      781 gccgacttga gcatcggctc cagctcgccc cgctcatgca gttcgtgcac gatatcgcag
      841 ccgccgacga attttccgtc tatataaacc tgcggaatgg tgggccagtt cgaatacctc
      901 ttgattccat cccgcacagc agcatcggcc aagacatctg ccgtagcaaa cggcaccccg
      961 taggatctta agatctctac cgtgtgagcc gaaaagccgc actggggaaa atccggcgtg
     1021 cccttcatga acagcatgac cttgttgctt ttgaccttgg aatcgatttc gttcaggaca
     1081 tcttctgcca taatccttct ccttatttat ctgcgaacct atgatccaag cttcgcccac
     1141 tgctccggtg taaaggtttt gagcgcgagc gcatgaatac gctgatcgcc catctcctcc
     1201 ttaagcgccg cataaaccat ctgatgttgc tcgatcaacc ccttaccctc gaacgctggc
     1261 gagacgatga acacttgcca gtggtctccg cctccggtga gatcctgcat ttcgatggtg
     1321 gaaccaggaa gcgcttgttc cagagtgctt ttgatttcgt ccggagtaat cataggcaaa
     1381 tcttatcccc ttgattcgcc cctttcaagc tcaatcggcg gagctttgct ttaagcttga
     1441 ggatcgaagt cgctccgcac tttcttcatc atggcgcgca agtccatgcg cagctcgtcg
     1501 agagtcggac ggccgacgta ccagtaaccg cagtaaaggt tgtgaaccgt caaatccggc
     1561 atcaacgaaa aggtatatgg gattgctgtg acgccgcgcg atttcgaagt ttcggtcatg
     1621 tccagcgctt gaacgacttt gcgatcgtga tcgctcaaaa ttgggaactc ggcgcccaag
     1681 ccagttcgaa aggccgaatt gacttccggc ggatcaacgc tcacgaccac cagcttacag
     1741 tagttaatcg ccagctctct ttggaattcg acataatggc gcaactgcac ctggcatttg
     1801 cctcaccagt aaccgcgata gaacgacagg atcaagggaa acttgccggc atactgagaa
     1861 agcttgaccg gttggctgtc atgatcgagc atttggaagt cggggaattg ctttccaatg
     1921 gtttggttca taactctctc tcctgaatca atgagatcac acgggcgaat caacaaatca
     1981 cgactacgga tagcgctttg ccatctcgtc aaagaatccc tctttctcca gctgttgaag
     2041 caaactgaga tcgacgaact gttcgggttt ggcagttttc gcctgcggat ttttcacagc
     2101 tagatcatca agcaaatact gaattccttt gagggttgga tagggccgct tggcaacact
     2161 tttgacgaag gcggtgtaag tcgcttcgag agcttcggca tccgcgccgc gcaggtagtg
     2221 accgacgatt ttcttggttt cttcccggtt cttatggata tagtggatcg attcaacgta
     2281 gcccttcaac gcgctcaaag ccgtttcccg ctgattgcga ataaacgtcc gggaagtggc
     2341 aaagccgttg ccttgcacct cgattcctag atccagcatg tcaaagatct caatgaagcc
     2401 ctgcttgcgc gccctgggtg tttccgacgg agtcaagata gtcgcgtcga tgcgattgga
     2461 ctccatcgcc gccaatcggt cgggagtcgg acccgaaacc acgagcatga cgtctttgtt
     2521 gggttcaaga ttgaacttct ttagaagaat caatgcggcg gtatgcgatg ctgatccgaa
     2581 accgcttacg cccaatcgtt tacccttgag ttgctctagc ttcgttgtcg cgggacgcgc
     2641 cacgacggag tagttgtagg tactctcgga ggagccaatg agtacgacgt cgtatccctg
     2701 caatcccgcc aatactactg tgccgatgtt gacaatcggc ggatcgccgg caaccagtgc
     2761 ctgagttgcc attccgccgc cacggaaaaa aacgacctcg gcatccaggc catacttgcg
     2821 aaacaagccc gcctctttgg ctacccagat atggatttga ttgtaagata cggcggggta
     2881 gccgatcttg atctttttaa gctcttgcgc ctgagccagc gtcgcggccc atagcccaca
     2941 gagaaccagt gcggccactc gaatcgcgtt catttcttcc tttccatctt tgaagtaatg
     3001 tctgcccggc aacgctgtcc gagagcttgt cttcagccgc atcagcaatt gcgccctgct
     3061 acggccgccg atacggttaa aatcaaagtt ccgccttgcg cgctataacg gccgatatca
     3121 gagccgcttg gttcattccg cgatcacgag cgtgaccctg cttgttgcga tgctttcact
     3181 catccaggaa tgaaagcatt agcttcagta aagatcgcgg aacaggtcaa tgcgcccgct
     3241 ctgaccagtc tttagcctcg aacatttgac cggtgacatt ttctgactgc gcagcagcca
     3301 aatgaacgaa gatcggcgta atctgttccg gtgtcggtaa cgtcagcggg tcttcttcag
     3361 gataggcctc cgcacgcatc ccagtccgag taggccccgg attgacgctg ttcacccgaa
     3421 tcccaaactc cttgacctcg tcagccataa cctgagtgag tccttccagc ccaaactttg
     3481 acaccgcata ggcgccccac cgagcccgac cgacacgccc aacgccggat gagacattga
     3541 taatggaacc atgacgttgc gccatcatac gtttgaccac ctgctgggat agaagaaata
     3601 caccgtgtaa gttgacccga atcacctctt cccattgccc aaatggataa tggacgagag
     3661 ccacccgcgg gccgagcagg cttgcattgt taaccagcgt gtcaacggtt tgaaatcttt
     3721 cgatggcggc atcgacgatt cggcgcacat cgttttcctt gccgacatca cctgcgcaac
     3781 catccacctg tgcgtccgtc gtgtcctcac gcaattcgcg caccgcggcc gcaacctcct
     3841 gccggttgcg cgcgcagata aacacgcggg cgccctcacg caggtaggcg gcggcgatgg
     3901 ctctgccgat gccgcgagtg gctccggtga tcagagcaac attgttcatc agttttttcc
     3961 gatccgtgct t
//
LOCUS       DASUTG010000789         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2196993, whole genome shotgun sequence.
ACCESSION   DASUTG010000789 DASUTG010000000
VERSION     DASUTG010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196993"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..808)
                     /locus_tag="VF089_18260"
     CDS             complement(125..808)
                     /locus_tag="VF089_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX6175960.1"
                     /translation="MQRLFVYLVFGALSLGANAVWAQTPASAMPASSVASFEENIAAF
                     AAADREQAPPTGGILFVGSSSIRLWNDLEKQFGPSIIKRAVGGSKMSDCTRYLDRLVF
                     PYKPRQVVVYAGDNDLAEGIPPEDILISFQKFVDGVQRELPSTRISYISIKPSPARAR
                     LIPKVRETNHMIQKYTSQHEKLDFIEVFTAMLDAEGQPRADLFRADALHLNEAGYTLW
                     RKIIAQHVR"
     gene            1220..2968
                     /locus_tag="VF089_18265"
     CDS             1220..2968
                     /locus_tag="VF089_18265"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6175961.1"
                     /translation="MFENRLFEPGQLLNLLETAVGQAKEAVLITSARLDPPGPQIVFI
                     NPAFSEMTGYSEEEVLDKTPRILQGPKSDRAMLQRLREALGRGQPFSGETVNYRKDGA
                     EYYVEWDITPIRDSAGQIKYFLSIQRNVTERKLAENQLRELLCQIERSRNDLDSILHA
                     LLIGAVMSDENGQVVFINTAARHLFGRDGLGLAWKDLFGLNSEDSRNCKMLTEKPAEE
                     RTRIPLHFDRSDGRRIWLEVDVKDDPRDARGKIFFLYDVTEVHDLRRLLDDRAQFHDL
                     LGKSKAMQQVYQQIRDLAKVDSTVLIEGETGTGKELVARAIHASSHRKDKPFIAVNCA
                     GLTESLLSSQLFGHRRGAFTGAIDDQQGFLEAANRGTLLLDEIGDIPIAVQNQLLRVL
                     QEREIVRVGESRPRKIDVRVLAATHRNLSDEAAKGNFRSDLFYRIRVARITLPPLRER
                     RDDIPLLTASFLAQLSAASKERVTEVSDDALRLITGYPWPGNVRELRSAIEFAVIRCR
                     GTVVQEDDLPPEIFEPADIRGALRGDPLNDEKARLVDALGRSRGNRARAARLLGISRA
                     TLYRRLSDLKINPEEK"
     gene            3185..3646
                     /locus_tag="VF089_18270"
     CDS             3185..3646
                     /locus_tag="VF089_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017036629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6175962.1"
                     /translation="MVLALTLGVTDLAKAESMDKDIVEVAASAGSFKTLIAAVQAAGL
                     VEALKGQGPLTVFAPTDEAFAKLPAGTVENLLKPENKDKLAAVLTYHVVAGRVTAGEV
                     VNLSSAKTINGRALQISSMDGKVMIDNARVTATDIMASNGVIHVIDSVVLP"
BASE COUNT          891 a          988 c         1101 g          850 t
ORIGIN      
        1 gaaaccccgc tatgggttga tgtcgcgttg attgccggta gcttcgcgct tctatataca
       61 gtcgcccgag ttttcaatcc gatgaagccg cgcagagacg tggaaaagga ggaatcggcc
      121 gggctcaccg cacatgctgc gcgatgatct ttctccaaag cgtgtagccg gcttcgttca
      181 ggtgcagcgc gtcggcgcga aagagatcgg cgcgtggttg tccttcggcg tcgagcatcg
      241 ccgtgaacac ttcgatgaag tccagctttt catgctgaga ggtatatttt tggatcatat
      301 ggttggtttc ccgaaccttg ggaatcagcc gggctcgggc cggacttggc ttgattgaaa
      361 tataggagat ccttgtgctt gggagttctc tttgtacccc atcgacaaat ttctggaagc
      421 tgatgaggat gtcctccggc ggtatccctt cggccagatc gttatcgcca gcgtaaacca
      481 cgacctgacg cggcttataa gggaagacca atcgatctag gtaacgcgtg caatcggaca
      541 tcttagagcc gccgaccgcg cgcttgatga tggacggccc aaattgcttt tcaagatcat
      601 tccacagcct aattgacgaa ctgccgacga atagaattcc tcctgttggc ggggcttgct
      661 cccggtctgc agcggcgaag gcggcgatat tctcttcgaa cgatgccacg ctggacgctg
      721 gcattgccga agccggcgtt tgagcccaaa ccgcgttagc gcccaatgaa agggcgccga
      781 atacaagata gacaaacaat ctttgcatga agtaatctgc tgcgcttttt cgaaccgatc
      841 gaagtcagcc tcgattaaac tgtctcgctt ccggaattga ctggccattc aaacctcagc
      901 tcgccattcc ttcggcctgt aatatcctaa tttccgccac ctctgtcaat gacgcacggc
      961 ggccgatcgg ccgagcagtc cggcgaagta agtagcagca agatcgtgcc attagccatg
     1021 tcgcattacc tcgcgacatg cagattcatg atcgactttt agctacggca gaactcggaa
     1081 cccaagttcg agctgtattc cccctggaaa gtcattgcca gttgcagatc gaaggtttta
     1141 gttcgaggat taacgaacgg atgcgataac ggaataagaa gtttgaatca accgagacca
     1201 aagcgtagga gacaatcggg tgtttgaaaa ccgtctattc gaaccggggc agcttctgaa
     1261 tttgcttgag accgctgtcg ggcaggccaa agaggcggtg ctgatcacgt cggcgaggtt
     1321 ggatccgccg gggccgcaga ttgttttcat taatccggcg tttagtgaaa tgactggata
     1381 cagtgaagaa gaagttctcg acaaaacacc gcggattctt caaggtccga agtccgatcg
     1441 cgccatgttg caaaggctgc gcgaggcact cggtcgtgga cagccgtttt ccggcgagac
     1501 ggtcaattac cgcaaggacg gcgccgagta ttatgtcgaa tgggatatta caccgattcg
     1561 tgactcggcc gggcagatca aatactttct ctcgattcaa agaaatgtca ctgagcgcaa
     1621 gttagctgag aaccagctta gagaattact ttgccaaatc gagagaagcc gcaatgactt
     1681 ggattcaatc ctccatgcgc tcctaatcgg cgcggtgatg tccgatgaaa acggtcaggt
     1741 ggtgtttatt aacacggcag ctcggcatct ttttggccgt gacggactcg gcttagcctg
     1801 gaaggacctc ttcggcttga actccgaaga tagccgtaac tgcaagatgc tgacagaaaa
     1861 gccggcggag gagcggacgc ggattccgct tcactttgat cgaagcgacg gccggcgcat
     1921 ctggctcgaa gtagacgtca aagacgaccc tcgagatgcc cgcggaaaaa tcttctttct
     1981 ctatgatgtc acggaagttc atgacctccg ccgccttctc gacgaccggg cgcagtttca
     2041 tgatttacta gggaagagca aagccatgca gcaggtctac cagcagatcc gcgacctggc
     2101 gaaggtggac tcgaccgtat tgatcgaggg cgaaaccgga acgggtaagg agctcgtcgc
     2161 tcgcgcgatt catgcgtcga gccatcgcaa agataaaccg tttatcgccg tgaactgcgc
     2221 aggtcttaca gaatcgcttc tgagcagcca gctcttcggc cacaggcgcg gagcttttac
     2281 cggcgccata gatgaccagc agggattttt ggaagccgcc aatcgaggca cgctgttgtt
     2341 ggacgagatt ggcgacatac cgattgccgt gcagaaccaa ctgctgcgcg tgcttcagga
     2401 gcgcgagatc gtccgggttg gagagtcacg cccgcgaaaa attgacgttc gagtgttggc
     2461 ggcgactcac cgcaatttga gcgatgaagc agccaaaggc aacttccgtt cggacttgtt
     2521 ttaccgtatc cgggtggcgc gaatcacttt gccgccgctg cgcgagcggc gtgacgatat
     2581 ccctttgctg actgcgtcgt ttctcgcaca gctctccgca gccagtaagg agcgcgtgac
     2641 ggaagtgagc gatgatgcat tgcgcttgat tacgggttac ccatggcccg gcaatgtgcg
     2701 cgagctcaga agcgcaatcg agtttgccgt gattcgctgc cggggcactg tggtccagga
     2761 agatgatttg ccgccggaaa ttttcgagcc ggcggatata cgcggcgcgc ttcgaggtga
     2821 tccgttgaac gacgagaaag cgcgtttggt cgatgccttg ggtcgttccc gcggaaaccg
     2881 tgcgcgggcg gcgcgcctcc tcggcatcag tcgggctacc ctttaccgcc gtttatccga
     2941 cctcaaaatt aatcccgaag aaaaatagtc tcacctgttt cagtcgagac atcgttgaga
     3001 ccacactgag acattttact gtcgcagtgc gttttgcgat ttcgttttac atccgcacaa
     3061 acagcagcct gacagccgca ggccaacgag tttcacactg gcaccgcggt tgctcatgtc
     3121 caggttcgaa gattcataaa aaataaaaag gaggaaaaaa atgagagtct tcaaaactgc
     3181 attgatggta ttggcattga cgttgggagt caccgattta gcgaaggctg aatcgatgga
     3241 caaggacatc gtcgaagtcg ccgcttcagc gggatcgttc aagaccttga tcgctgcggt
     3301 gcaagccgcg ggactggtcg aagcgctaaa agggcagggc ccattgacgg tattcgcgcc
     3361 aaccgatgag gcatttgcaa agttgccggc gggcacagtg gaaaacctgc tcaagcccga
     3421 gaacaaggat aagctcgcgg cggtgctgac ttatcacgtg gtagccggcc gtgtgaccgc
     3481 tggtgaagtg gttaacttga gcagcgcgaa aacgatcaac gggcgcgcgc ttcagatcag
     3541 cagcatggac ggcaaagtga tgatcgacaa cgcgcgtgtg acggcgacgg atattatggc
     3601 gagcaatggg gtcattcacg tgatcgattc tgttgtactg ccgtaaacgg ttactctctt
     3661 ggcggcaacc gcggctggat gttttccggc cgcggttttt cttggcgcta cgataatccg
     3721 ggaaccgtga tgagaagccc gctgcaagaa gcaagtcaca gacacatgga tcctacgatc
     3781 cgattgaagc tagcgtctag gtaatgtcac aatgtggttt ttgccagaga
//
LOCUS       DASUTG010000790         6804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1601346, whole genome shotgun sequence.
ACCESSION   DASUTG010000790 DASUTG010000000
VERSION     DASUTG010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601346"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF089_18275"
     CDS             complement(<1..250)
                     /locus_tag="VF089_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEX6175963.1"
                     /translation="MKSHRKELWFNTSTRVAFINITGQVEECIRESGVEDGLVLVNAM
                     HITASVFINDDEPGLHHDYGVWLEKLAPHEPLKQYRHNN"
     gene            394..1854
                     /locus_tag="VF089_18280"
     CDS             394..1854
                     /locus_tag="VF089_18280"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HEX6175964.1"
                     /translation="MSEIRFIDTTVRDGNQSLWALNMRVGAMLPIAEQMDQAGFESME
                     FFLSVMFKKYVREHKENPWEWVREGTKRIKKTRLRYHGGMHSAFEKTPHCILKLLIER
                     LVSYGLTVTRTSNSWNDFADFKEEITDLKKTGMDTVANLIYSVSPRHTDEYYARKAKE
                     AAASNPWRICFKDVGGLLTPDRARTLIPIVLESAGKIPVEFHAHCNSGQAPLCYLEGV
                     KLGMRILHTAVPPLANGSSQPSIFNIAHNLRALGYTPMVDLKPLQAVEKHFTAVAKKS
                     GLPIGKPVEYDHSQYLHQVPGGMISNMRHQLKIVGMEHKMEAALEEAAHVRKDFGYPI
                     MVTPLSQYVGTQAAINVLLGERYKEVPDQVIQYALGFWGKEAVEVMDPDVRDKILSRP
                     RAKEWTHWEQPEPTLHEVRQKLGVHLSDEELILRFFAGNDYVDALPDGGKPREYLNGD
                     LPLVQLIEQLSQRKGSNQIFIKRPGFSVRMEKRTAS"
     gene            complement(2272..2913)
                     /locus_tag="VF089_18285"
     CDS             complement(2272..2913)
                     /locus_tag="VF089_18285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6175965.1"
                     /translation="MDQTQFIIVRHGQTQWNYRGVRQGHLDSDLTERGIAQAKALAIR
                     LARERFTRLYSSDLGRALRTAQIIAASTGHEVVTDPRLRERHLGIFQGLNGEEIRARY
                     PDEHRLHRSVGPDYVIPGGESVRQQVARNVAYLIEIARKHSGETLVVVTHGGVLSGLF
                     RHTFSIPFDAPRRFEFTNAGLNVFSYRDGDWFLQTWGDTSHLCEVGTVDGDDP"
     gene            3237..4052
                     /locus_tag="VF089_18290"
     CDS             3237..4052
                     /locus_tag="VF089_18290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175966.1"
                     /translation="MFAKIRHLAMYTHNVPAVTHFYQRIFGLKRLTSGTVDESGKQNA
                     NRGHISDGVIGMAILPRYAGIQSGMDHYGFEVTDIKDFVRRMEQYYPDVKIAKGLEYV
                     PFAGLRSYDPAGTQFDIAQAHIGNVREGYNESGWDQSRWINHISIRADNPMAVAEFYE
                     KVFELKEAETYRDDGSICMTDGKVKLLLRPRDNALYRGLREGLDHVGFKVENLKAAAD
                     DLDALAASSPDSAPRKLTVGLHGQLIENDFRKCCLGQHFISDPDGVLIDMSEN"
     gene            complement(4199..4876)
                     /locus_tag="VF089_18295"
     CDS             complement(4199..4876)
                     /locus_tag="VF089_18295"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX6175967.1"
                     /translation="MKIPNVPVPEILPIFPLTGVLVLPGTVLPLHIFEPRYRNMVEDA
                     LETDKVLGMIQPFVPQPDNQPRPGAEKESPDLYKIGCAGYIEKYERSADGRFFIQLKG
                     INRFRFEEELELRRGYRRVKAIYSEFPDATLPEGWNCERDAILQALTLYGQAHGMQVK
                     PEQAQRFSDLELVNLLAVSLPFHPAEKQALLEAPTLKDRENTLINLLRLGGQADPDSD
                     TTPRTLN"
     gene            5046..5477
                     /locus_tag="VF089_18300"
     CDS             5046..5477
                     /locus_tag="VF089_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6175968.1"
                     /translation="MVEFAPMDISTAKEFLARHHWGVLTTRRKEGALQMSPITVGIDP
                     DERAIISSRETAYKVNNLRRDPRAAVCVFTTEFHGEGWVQINGNAEILSLPEAMDTLV
                     YLERQAKGENRDWPSFHERMAREKRVIIRIRIDDVGPKRRG"
     gene            5495..6136
                     /locus_tag="VF089_18305"
     CDS             5495..6136
                     /locus_tag="VF089_18305"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX6175969.1"
                     /translation="MKQLRIENSWPSRLWTPLRCVLLLIVALVQPAHRLAAQQASDFV
                     GQLLVAMPEMKDPRFVESVIYIVQHDREGTLGLLINRPLARAPIEDLIKGSGIDGKNG
                     TREVTVHYGGPVNPRQGFVLHSNDFVLENSKEVKDGIAMTSDARIVNEISIGKGPRQF
                     LLMIGYAGWAPGQLEEEIKAKSWFLIPADKDLIFDKKTEQKWQAAMDKRQIRL"
     gene            6148..6570
                     /locus_tag="VF089_18310"
     CDS             6148..6570
                     /locus_tag="VF089_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX6175970.1"
                     /translation="MKLDLAKANIIVDAALENARKCKIRPLCIAVLDDGGNLKALKRD
                     DGASILRPQIAISKAWGAIGMGESSRHLGNRLKELPAFLNALSAMTGGKVVPVAGGVL
                     IMDGEVILGAAGASGATADEDEACVITGIQAAGLQFKV"
     gene            6602..>6804
                     /locus_tag="VF089_18315"
     CDS             6602..>6804
                     /locus_tag="VF089_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175971.1"
                     /translation="MKIVRLYTGNDQKSHFEEIELNFGGNQPMLTTESRAATSAIFRS
                     APAGLFLDRHPAPRRQFLVTLSGS"
BASE COUNT         1646 a         1841 c         1851 g         1466 t
ORIGIN      
        1 tattgttgtg acggtattgc ttgagcggct cgtgtggcgc cagcttttcc agccagacac
       61 catagtcgtg gtgcagaccc ggttcgtcat cattaatgaa gaccgaagcg gtgatatgca
      121 tggcgttcac caggaccaaa ccgtcctcga caccgctctc tcgaatgcat tcctcaactt
      181 ggccggtgat attgatgaat gcgacccggg tcgatgtatt gaaccatagt tccttgcggt
      241 ggcttttcat aaagctgctc ccggctttcc tatttgatct atcctgtaag aatttataca
      301 aagtataaga gcatcagtac accgccggcg accacgtggt tgtcatttga acatttgaac
      361 cgttgaacca gtttcccgga ggttagaggt tagatgagcg agattcgatt tattgacacc
      421 accgtgcgtg acggcaacca aagcctatgg gcgctcaata tgcgagttgg cgcaatgctg
      481 ccgatcgccg agcagatgga ccaggccggc ttcgaatcca tggagttctt tttgagcgtt
      541 atgttcaaga agtatgtacg cgagcacaag gagaacccat gggagtgggt acgcgaaggc
      601 accaagcgca tcaagaaaac tcgcctgcgc tatcatggag ggatgcacag cgctttcgag
      661 aaaaccccgc attgcattct caagctgctg atcgaacgcc tagtctccta tggccttacg
      721 gtgacgcgaa catcgaactc ctggaacgac ttcgctgatt tcaaagagga aattaccgat
      781 ttgaagaaga ccggcatgga tacggtcgcc aacctgatct actcggtttc gccgcgtcac
      841 accgacgaat attacgcccg gaaggccaaa gaagcggccg cgagcaaccc ctggcggatc
      901 tgcttcaagg atgtcggcgg tttgctgacc ccggatcggg ctcggacttt gatccctatc
      961 gtgcttgaga gcgcgggcaa aataccggtt gaattccacg cccactgcaa cagcggccag
     1021 gcgccgttat gctatctcga aggcgtcaaa ctgggcatga gaattcttca taccgcggtg
     1081 ccgccgttgg ccaacggttc gtcacaacca tcgattttca acatcgccca taacctgcgc
     1141 gctctcggct atactccaat ggtggatttg aaaccgcttc aagccgttga aaaacatttc
     1201 accgccgtgg caaaaaaatc cggcttgccg atcggcaaac cggtcgagta cgatcactca
     1261 caatatctgc atcaagtccc gggcgggatg atctccaaca tgcgccacca gctcaagatc
     1321 gtcggtatgg agcacaagat ggaagccgcg ctcgaagaag ccgctcacgt ccgaaaagat
     1381 ttcggctatc cgatcatggt gactccgctg tcgcagtacg tcggcactca agcggcgata
     1441 aacgtccttc tcggcgaacg ttacaaagag gtgcctgatc aggtgattca gtatgcgctg
     1501 ggcttttggg gcaaagaagc cgtcgaggtc atggaccctg atgtcagaga taaaatttta
     1561 agccggccac gcgccaaaga atggacccat tgggaacaac ccgaaccgac gctacatgaa
     1621 gtcagacaga agttaggcgt ccatctttcc gacgaagagt tgatcttgcg ctttttcgcc
     1681 gggaatgact acgtcgatgc attgccggac ggcggcaagc cgcgcgaata tctgaacgga
     1741 gatctgccct tggtacaact gatcgagcag ctcagccagc gaaagggctc gaatcaaatt
     1801 tttatcaaac gacccggttt ctcggtgaga atggaaaagc gcacagccag ctagccggct
     1861 gccgataaac tggaacctaa gttgagctaa tagcctggtt ctataaatcg gctttgttca
     1921 agcgattcaa aacgttccaa gtgttgaagt cgtgcaacca aactctcgaa tcttgatctc
     1981 ttggacgttc agcaactttg ctcgccgatt aaaaatcgct cccgcacgct ggtcaaaacg
     2041 ttcagatgca aggcacgtga agtctgacga gcgtaggcgt actctggtgt acgccggagc
     2101 ggagtcggac gagcggaacg cagcagctgg acgttttcac cagtgtgcac gctggtcaaa
     2161 tcgttcagag gtaaggcgcg cgaagttcgg tgagctgagg cgtacgtcgg caatacgtcg
     2221 aagcgaagcg aacgagcgga acgcagcagc ttagcgtttt caccagcgtg cctaaggatc
     2281 gtcgccgtcc accgtaccca cttcgcacaa atgactcgta tctccccagg tttggaggaa
     2341 ccagtcgccg tcccggtaac tgaacacgtt caatcccgcg ttggtgaatt caaaacggcg
     2401 cggcgcatcg aacggaattg aaaaggtatg gcggaagagg ccgctcagca cccctccgtg
     2461 ggtgacaacc actaatgtct cacccgaatg cttccgggca atctctatca agtaagccac
     2521 attgcgcgcc acctgctgcc gcacgctttc accgccggga atcacataat ccgggcccac
     2581 ggaacggtgt aggcgatgct cgtccggata cctggctctg atctcctcgc cgttaagtcc
     2641 ctggaaaatt cccagatggc gctcacggag gcgaggatcg gtgacgacct cgtggccagt
     2701 cgaagcggca attatttgag cggtgcgcag agcgcgtccc agatcgctgc tgtaaagccg
     2761 cgtgaaacgt tcgcgggcca agcgaatagc caaagccttg gcttgggcga tgcctctttc
     2821 cgtcagatcg ctatccaggt ggccctgacg tacgccgcga taattccatt gcgtctgccc
     2881 gtgccgcaca atgataaatt gagtttgatc catcgaattt aaacctcatc gacaatgctt
     2941 actcgcacgg tgccggtgcc gatgtcaaat atcacatccg gagttgtgcg cgaaccatca
     3001 accagcttca aaatgaccat ctttccaaca aaaggtcgat cgccagaact caatattccg
     3061 gcgcgcgaca tcccaacgtt ttttggctgt cgcgatacag ctcacagagc gattcggagt
     3121 tttgacggtt gagcaatccg atgacagcca aaaagtaacc aggcttcaat ccggatgatt
     3181 tgacaaacac cttttcctaa cttagatgga tcgttagtat cgcaaggagg tgcagcatgt
     3241 ttgcaaaaat ccgccatcta gcgatgtaca ctcataatgt gccagccgtc acgcatttct
     3301 accagaggat ctttggcctg aaacgactga ccagcggcac tgtcgatgaa agcggcaagc
     3361 agaatgccaa ccgcggccac atcagcgacg gcgtcatcgg catggccatc ctgccacgat
     3421 acgccgggat acagtcgggc atggaccatt atggattcga agtcaccgat atcaaggatt
     3481 tcgttcgccg gatggaacaa tactatccgg acgtaaagat cgccaagggt ttggaatatg
     3541 tgccgtttgc cggattgcgc agctacgatc ccgccggcac ccagttcgac attgcgcaag
     3601 cgcatatagg caatgtccga gaaggctaca acgaaagcgg ctgggatcaa tcgcgctgga
     3661 tcaatcatat atcgattcgc gctgataatc cgatggcagt ggcggagttc tatgaaaagg
     3721 tcttcgaact aaaagaagca gagacttacc gcgacgacgg atctatctgc atgaccgacg
     3781 gaaaagttaa actcttgctt cggcctcgcg ataacgccct ttaccgcggc ctgcgagaag
     3841 gtctcgacca tgttggattc aaagtggaaa atctcaaagc ggcggccgat gatctcgatg
     3901 cactagccgc ttcttcgccg gattcagcac cacgaaaact caccgtcgga ctgcatggcc
     3961 aactgatcga aaacgatttc cgcaaatgtt gtctaggtca acacttcatc tcggatccgg
     4021 atggtgtgct gatcgatatg tcagaaaatt gaaacttctg agctcagcgc cgcaggctgt
     4081 tcaaaagatc tcataggcaa ggcgcgcgaa gatcgacgag ctgaggcgta cgcttcagta
     4141 cgttgaagcg agtcgaacga gcgcaacgca gcatatgagt cttttcagcg gcctgagatc
     4201 agtttagggt gcggggtgtt gtatccgaat ccggatccgc ctgaccgcca agtcgaagca
     4261 gattgatcag cgtattctcc ctgtctttca gagttggcgc ttccaataat gcttgtttct
     4321 ccgccgggtg aaacggcaac gagacggcca gaagattcac aagttccaaa tccgagaagc
     4381 gctgcgcctg ctccggtttg acctgcatgc cgtgagcttg accatataac gtcagcgcct
     4441 gcagtatagc gtcgcgctcg cagttccaac cctccggcag ggtggcatcg ggaaattcgc
     4501 tataaatggc tttgacacgc cgataccccc gtcgtagttc gagctcctct tcgaatctaa
     4561 agcggttgat acctttgagc tgaatgaaga agcggccgtc agcagatctc tcgtatttct
     4621 cgatgtaacc ggcgcaaccg atcttgtata gatccggtga ttccttttcg gccccgggcc
     4681 gcggctgatt atccggttgc ggcacgaacg gttggatcat gccaaggacc ttgtctgtct
     4741 ccagagcatc ttccaccatg ttgcggtagc gcggctcaaa gatgtgcaat ggcagcactg
     4801 ttccgggcag aaccaaaact ccggtcaagg gaaagatcgg cagtatttcc ggcaccggca
     4861 catttggaat tttcattgcc acagaattcg tctcgtcacc tgtctgttta taggtgatgg
     4921 cgcaagaacg gtcaaggtga tgatacccgg cagggtcctg agaacccgag ggcaatatgc
     4981 tcttgcaata acgtgctcgg ctattctagc ttgtcaaaag tttagctgca gcctatagga
     5041 ataccatggt tgaatttgcg ccgatggata tctcgaccgc gaaagagttc ttggcccggc
     5101 atcactgggg cgttctgacg acccggagaa aggaaggcgc cttgcagatg tcaccgatca
     5161 cggttggcat cgatcctgac gaacgcgcca tcatcagcag cagggaaacc gcgtacaaag
     5221 tgaacaatct gcggcgcgac ccgcgcgcgg cagtgtgtgt cttcaccacc gagtttcacg
     5281 gcgaaggctg ggtgcaaatc aacggcaacg ctgaaattct atcactgccg gaggcgatgg
     5341 atacattggt gtacttggaa cgccaggcca aaggcgagaa ccgggattgg ccgtcgttcc
     5401 atgaacgaat ggcgcgtgag aaacgggtca tcattcgcat ccgcatcgac gatgtgggtc
     5461 cgaaacggcg cggttaggca ggccgggtgc tttcatgaag caattaagaa tcgagaactc
     5521 atggccgtca cgtttatgga ccccgctccg ctgcgtgctg ctcttgatcg ttgctctggt
     5581 tcagccggcg catcggctcg cggcgcagca ggcgagcgac ttcgtcggtc aattgttagt
     5641 cgccatgccg gagatgaaag atccacggtt tgtcgagtcg gttatttaca tcgtgcaaca
     5701 cgatcgtgaa ggcacgttgg gtcttctcat caatcggccg ctggcgcggg cgcctattga
     5761 agacctgatt aaaggctccg gcatcgatgg caaaaacggt acaagagaag tcaccgttca
     5821 ctatggcggg ccggtgaacc cgcgccaagg tttcgtcctt cacagcaacg acttcgtact
     5881 tgagaactcg aaagaagtta aggatggcat cgccatgact tcggacgcga gaatcgttaa
     5941 cgaaatttcc atcggcaaag gtccgcgcca attcttgctg atgatcggtt acgccggctg
     6001 ggcacccggc cagctcgagg aggagatcaa agccaaatca tggtttctca tccccgctga
     6061 taaagacctt atcttcgaca agaaaaccga gcaaaagtgg caagcggcga tggacaaacg
     6121 gcaaattcga ctttgacgag gagagctatg aaacttgatt tggctaaagc gaacatcatc
     6181 gtcgatgcgg cgctggagaa tgcgcggaag tgcaagatca gaccgctctg cattgccgtt
     6241 ctcgacgacg gcggcaactt aaaagctctc aagcgtgatg acggcgccag catcttgcga
     6301 ccgcagatcg ctatcagcaa agcctggggc gcaatcggca tgggagaatc gagccgccat
     6361 cttggcaatc ggctgaaaga gcttccggct tttctcaacg cgctatcggc catgaccggc
     6421 ggcaaggtcg ttccggttgc cggcggcgta ttgatcatgg acggtgaagt gattctcggc
     6481 gcagccggcg ccagcggcgc gaccgccgat gaagacgagg cttgtgtcat taccggcatt
     6541 caagcagccg gccttcaatt caaagtttga tgcgcagcta aattattcac aggagcacat
     6601 aatgaagatc gtcaggctgt atacgggtaa cgatcagaag tcgcatttcg aagaaatcga
     6661 gctcaacttt ggcggcaatc aaccgatgct gacgaccgaa tctcgagccg caaccagcgc
     6721 gatctttcgc tccgcgcccg ccgggctttt tctcgatcgc cacccggcgc cgcggcgcca
     6781 attcctggtg acactatccg gctc
//
LOCUS       DASUTG010000791         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1011365, whole genome shotgun sequence.
ACCESSION   DASUTG010000791 DASUTG010000000
VERSION     DASUTG010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011365"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /gene="glnD"
                     /locus_tag="VF089_18320"
     CDS             <1..2064
                     /gene="glnD"
                     /locus_tag="VF089_18320"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX6175972.1"
                     /translation="VKESEGGLRDIHTARWMARVKMQAKDLDELAAKGIISAADIAKL
                     KASHDFLLRVRNELHFSTGRHQDQLTFEEQEKVSQALGFQGEGSLKAVEVFMSHYYRH
                     ASEISRLTALITHRLTEGGWPLLGGMVGLGRNIREGVRISRDHITITKPEILKRDRGN
                     LMRIFEDMQKHRCSLSHESRELLREHLDLIDDEFRASAAANLPFFRILKWKEGVYDAL
                     FEMHRAGVLGAFIPEFGRLLCMVLHDAYHIYTVDQHSLRLIQEVERLKAGEFKDSLPL
                     LTQLAREAEKIELLYLGLMLHDIGKGFGGGHSERGAIMVRRIAHRMRLNVDDGALVEF
                     LVRHHLLMTHTAFRRDLEDEKTICDFAQTMGSANNLKMLYLLTYADVRGVGPDVWNPW
                     KASLLGELYVKTLTVLEEMEKGEFHRQDVRAALGRIQTRVREELSATASPEEVDRFIE
                     VMPERYFLSMAENEIPAHFALMHEYKGHGAAVSVEHFPEKDCSTVAICTADRPGLFAS
                     IAGVMAAMRLDILNARIFTAKDGRILDVFRVSHGGRSEIAMAEQRWSRFRSTLEAVLD
                     GNLDVERLVEKSQSTLLRKRMPKVLTSIQIDNEASALYTVVEVYTADRLGVLFRITYT
                     LHQLGLSIHVAKISTNVDQVADVFFVTTAGGDKVEDFARIEAIRQTLYASLVPDDERI
                     AAPLH"
     gene            2033..2971
                     /gene="xerD"
                     /locus_tag="VF089_18325"
     CDS             2033..2971
                     /gene="xerD"
                     /locus_tag="VF089_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX6175973.1"
                     /translation="MMKESLHPYIDSFLTMISVEKGLAKNTVEAYSRDLSGLAGFMLE
                     QKVTRWDAVEPIHLRLFVSSLRARNLSSRSIARQIVTVRRFYRFLQTEGLVTRPPVPE
                     FHLSGTARKLPHALNGDDVRSLLNKPDPNRVLGARDQAMLELLYATGLRVSELVLLKL
                     QQIDFQGDYLTVKGKGSKMRAVPFGKWSREKLLRYIQETRPRLLKGKASVFLFVNRSG
                     QALTRQGCWKIIRGYARAAGIDKKVSPHTLRHSFATHLLAGGADLRSVQTMLGHADIS
                     TTQIYTHVDANRLKQAHQRYHPREGGAQGKPKPATD"
     gene            3144..>3335
                     /locus_tag="VF089_18330"
     CDS             3144..>3335
                     /locus_tag="VF089_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6175974.1"
                     /translation="MEETIRQIAIWALPVLLAIVFHEVAHGWVAYHLGDSTAARMGRL
                     TLNPIAHVDLFGTILIPLLL"
BASE COUNT          742 a          876 c          939 g          778 t
ORIGIN      
        1 gtcaaggaga gcgagggagg gttgcgggat atccataccg cgcgttggat ggcgcgtgtc
       61 aaaatgcagg cgaaagatct cgatgagctg gcagccaagg gcatcatcag cgcggcggat
      121 atcgcgaaat tgaaggcctc ccatgacttt ctcctgcgag tccgcaatga gctgcatttt
      181 tctaccggca ggcaccaaga tcaattgacc ttcgaggagc aggagaaggt gagtcaggcg
      241 ctgggttttc aaggcgaagg aagtctgaaa gcggtcgaag tatttatgag ccattattat
      301 cggcacgctt cagaaattag tcgccttacc gcgctgatca ctcatcgatt gactgagggc
      361 ggctggccac ttctcggcgg gatggtcggt ttggggcgaa atatacggga gggggtcagg
      421 atctccagag accatattac gatcaccaaa ccggagatcc tcaaaaggga tcgcggcaat
      481 ctgatgcgca tcttcgaaga tatgcagaag catcgctgct cgctcagcca tgagagccga
      541 gagctgctgc gcgagcatct cgatttgatc gatgatgagt ttcgtgcttc ggcggcagcc
      601 aacttgccgt tttttcgaat tctcaaatgg aaagaaggcg tctacgacgc tctgttcgaa
      661 atgcatcgtg ccggggttct cggcgctttc attcccgaat tcggcaggct cctgtgcatg
      721 gttttgcacg atgcctatca catctacact gtcgaccaac actcgttgcg cttgatccag
      781 gaagtcgagc ggctcaaggc tggagagttc aaggactccc tgccgctgct gacgcagttg
      841 gcgcgagaag ccgaaaaaat cgagctgctc tatctcgggc ttatgttgca cgacatcggc
      901 aaaggcttcg gtggcggtca ttccgaacgg ggcgccataa tggttcgccg gatcgcgcac
      961 cggatgcggc ttaatgtcga cgacggcgcg ctggtcgaat ttttggttcg acatcatctc
     1021 ttgatgacgc acaccgcttt tcgtcgcgat ctggaagacg agaaaaccat ctgtgacttc
     1081 gctcaaacca tgggcagtgc taataatcta aagatgctct acctgctcac ctatgctgac
     1141 gttagaggcg tggggcccga tgtttggaat ccgtggaaag cgtccttgct gggcgaactc
     1201 tatgtaaaga cgctgacggt cttggaagag atggagaagg gcgagtttca ccgtcaagat
     1261 gttcgcgctg ctctggggcg cattcagact cgcgtccgcg aagaattatc ggcgacagcc
     1321 tcacccgaag aggtcgatcg ttttatcgaa gtgatgccgg agcgttactt cttatcaatg
     1381 gccgaaaacg agatcccggc acatttcgcc ttgatgcacg agtataaagg acatggcgcg
     1441 gcggtttctg tcgaacactt tccggaaaaa gactgcagca ccgtagccat ctgcaccgcc
     1501 gatcgtcccg ggctgttcgc ttcaatcgcc ggggttatgg ccgccatgag attggatatc
     1561 ctcaatgccc ggattttcac tgccaaggat ggccgcattc tcgacgtctt tcgagtctcc
     1621 cacggtggac gctcggagat cgccatggcg gagcagagat ggagccggtt tcgctcaact
     1681 ttggaagcgg ttttggatgg caacctcgac gtcgaaaggc tggtggaaaa atcgcaatcg
     1741 acgcttttac ggaaacgcat gcccaaagtg ctcacctcga tccagataga taatgaagcc
     1801 tcagcccttt acaccgtggt ggaagtctat accgccgatc gcctcggcgt tttgttcagg
     1861 ataacctaca cgctgcacca gcttggtttg tcgattcatg tggcaaagat ttcgacgaac
     1921 gtcgatcagg tggcggacgt attttttgtt accaccgcgg gaggcgacaa ggtcgaagac
     1981 ttcgcgcgca tcgaggcgat tcgccagaca ctctacgcca gcctcgttcc cgatgatgaa
     2041 agaatcgctg cacccttaca ttgattcatt tctgacgatg atcagtgtcg agaaggggct
     2101 tgccaagaac accgtggaag cctatagccg cgatctctca ggattggccg ggtttatgct
     2161 cgaacagaag gtaacccgct gggatgccgt ggagccgatc catcttcgtt tgttcgtatc
     2221 atctttgcgc gcacgcaatt tgagcagtcg ttcgatcgcc cggcagattg taaccgtaag
     2281 gcggttctac cgttttctgc aaactgaagg gttggtgacc cgtcctcccg ttccagagtt
     2341 tcatttaagc ggcaccgcgc gaaagttgcc gcacgctctt aacggggacg atgtccgctc
     2401 tctcttgaat aaacccgatc ctaatcgagt tctgggagcg cgtgatcaag cgatgttgga
     2461 actgctctat gcaacgggac tgcgggtatc ggagttggtg ctgctaaagc tccagcagat
     2521 cgattttcaa ggcgactacc taacggtcaa aggcaaaggt tccaaaatgc gcgcggtgcc
     2581 gtttggcaaa tggtcgcggg aaaaattact tcggtacatt caagaaactc ggccgcgtct
     2641 gctaaagggc aaggccagcg tttttctatt cgtcaatcgc tcaggtcaag cgctcactcg
     2701 ccagggatgc tggaagatca ttcggggata cgcccgggcc gccggcatcg ataaaaaagt
     2761 ttcgccgcac actttgcgcc attctttcgc aacccatctg ctggcaggcg gcgctgatct
     2821 ccgctcggtg caaacgatgc tcggccatgc cgatatttcg acgacccaaa tctatactca
     2881 tgtggacgcg aatcgtctga aacaggccca ccaacgctat cacccgcgcg aaggcggcgc
     2941 gcaaggcaag ccgaaacccg cgaccgacta ggcggagttt tcaactttac ggcgcaccga
     3001 tggccccccc tccttaatcc tccccagcga gcgggagagg attaaggagg gggattcaga
     3061 tcctggagtt gctctcattt cagcttgtgt tttagaaagc tttaccatac gtttttaaac
     3121 tatttctcga aagatataga actttggaag aaaccataag acagattgcg atctgggccc
     3181 tcccggtatt gctggcgatt gtgtttcatg aggtggcgca tggctgggtg gcttaccatc
     3241 tgggcgattc gactgccgcg cggatgggcc gccttacctt gaatccgatt gcccacgtcg
     3301 atttgttcgg cactattctg attcctttac tgctg
//
LOCUS       DASUTG010000792         6765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1601611, whole genome shotgun sequence.
ACCESSION   DASUTG010000792 DASUTG010000000
VERSION     DASUTG010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601611"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VF089_18335"
     CDS             <1..1079
                     /locus_tag="VF089_18335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6175975.1"
                     /translation="LLRRGKFVETLLANITAGVIAVDPSGRITSWNKAAEQMLAVDRS
                     AALGKNYREAFENEPLVVMRDLIDNLKDRESSEAELQIQLPDHLVTVFATAATLRDDA
                     NESLGIMIFLEDITQIQKVQRMEAWREVARRIAHEIKNPLTPIQLSAERLRKRYAKLL
                     QGDGAVLDKCTTTIIQQVLELKNLVNEFSQFARLPTAQPVPNDLNLIIQDALFLFKEG
                     HNHIDFEFRQGDLPSLELDQEQIKRVMINLLDNAVAALADGGHVQISTAYDHARNTVY
                     LEVADDGCGIAPEARVRIFEPGFSTKKQGTGLGLTIVNQIVEDHRGYIRVRNNEPHGT
                     RMIIEFPVREQDALQESKRKAAQL"
     gene            1092..2393
                     /gene="lpxC"
                     /locus_tag="VF089_18340"
     CDS             1092..2393
                     /gene="lpxC"
                     /locus_tag="VF089_18340"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX6175976.1"
                     /translation="MNELSSQKTILVVDDEDKVRESVKEVLTDEGYRVLDTADATCVL
                     EMIEAEQPGLVLLDIWMPEIDGIGLLKEIKSKKPSTNVVMISGHGNIHTAVTATKFGA
                     FDFIEKPVSLDGLLHTVQRALGELCVTPSLAEVGYPAGDIPDSLSTARQIVTSAEPMI
                     RQRTLSKSVVSSGQGLHSGIKTGLILHPLPPNSGILFEGISGDVSVPAHLDYVGSTGF
                     ATSLSHRGFTVATVEHFLAVLHSYGITNLLVKVQGEVPILDGSAAEFCELIEEAGLHE
                     QDESWSRIVIDRTYRVGEDGGEWISIEPAETFSVHYVLKYPKPVGMQEYMFTHHSSSS
                     FKEEIAPARTFGFLRDMEKMQKLGLINGGRLSNCILIDDEKIVNTDLRFPEELARHKI
                     LDIIGDFYLLGRPIRGKITAHMTGHSDNIALLKAVRTGMEL"
     gene            2433..2882
                     /locus_tag="VF089_18345"
     CDS             2433..2882
                     /locus_tag="VF089_18345"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX6175977.1"
                     /translation="MDSSSPYSRLFTINEANALLPIVRPTIERILEIICRLRVQSEIV
                     IRAEQIDPETPNLMRRLQQNGEIADLIKQINDAVNEIQQHGCVCKGVEQGLLDFPCLL
                     GEEIVFLCWQYGEPEITYWHKIEDGFAGRRPLLDSTDTGSSGGRAVH"
     gene            2974..4119
                     /gene="queG"
                     /locus_tag="VF089_18350"
     CDS             2974..4119
                     /gene="queG"
                     /locus_tag="VF089_18350"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX6175978.1"
                     /translation="MSELSQRIKNEGQRLGFELVGISPVRVPPHEESFARWLRKKYHG
                     HLDYMERTELLRRDPRLLVPWAVSIISVGMNYYTPLKRVTPAERGRGWISRYAWGDDY
                     HEIIREKLEALLTFLREIGGDTVEGRAFVDSAPILERDFAGTAGIGWIGKNTQLISPK
                     KGSWFFLGELFTNIPLSYDQAIRDRCGQCDLCLKACPTGAFTGPYLLDARRCISYLTI
                     ELKGFIPRHLRPLIGNHIFGCDICQDVCPYNVKASSTVEPSYLPRQGLYAPELIGLLS
                     IGREEFRRRFRGSPILRAKRRGFLRNVAVALGNNAGPEAVPALTRALNDEESLVRGHV
                     AWALGRIGTSEALAALRQKLLSEDNPEVKSEIQEALGEATEPLANHS"
     gene            complement(4144..4587)
                     /locus_tag="VF089_18355"
     CDS             complement(4144..4587)
                     /locus_tag="VF089_18355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX6175979.1"
                     /translation="MSSSKPSTSNVTTYRVIYGDTDQMGVVYYANYLRWFERGRAELL
                     RACGIPYATIERRGMHFPVAELSCRYLRPAHYDDLIVIETRITSVSRASLTFTYRITR
                     EAEALLLASGSTKHACVDGQGRIMRMQHDLLRALEVMLCPAENTS"
     gene            4752..5219
                     /locus_tag="VF089_18360"
     CDS             4752..5219
                     /locus_tag="VF089_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175980.1"
                     /translation="MKRKGGFLTLSAGSLLLAVGLLACSSVQEETAAPAQKSPSAQAK
                     PAGEAKPATETKATKSETKQTKSKAVEVEGVISSVGQRTITFQVERKGKVREEVVGVD
                     DKTNIEKAGSRVKLKDLQETDKALINYEPEAYTPATAIKVIGQGKLQKVGGGD"
     gene            complement(5216..6106)
                     /locus_tag="VF089_18365"
     CDS             complement(5216..6106)
                     /locus_tag="VF089_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6175981.1"
                     /translation="MNGHLGLIHAIIPATVDIHASIPEDHPSTKNLGSERTGSGTIVD
                     PEGYILTVNYVVTGASSIDVTVAEGEEYPGEIVTQDLDSGLALLKIPARGLPFLKAST
                     EPAVLGQASLIVASSGNNERKVSGGYVTSLEPYDGHWEYMLDQTVRVTAVNPGFGGGT
                     LANFKGEMLGVVSLNLPDIGKFSLAIPISYYLDQEQAFKQRSLISQKPWLGFYPHLLA
                     GHVVVGGVTEGSPADRHGLKEGDIILDVESTQIRSRRQLYQELWKKRPGERISLRVLR
                     NETSIELNLVGADRRSRYGS"
     gene            6571..>6765
                     /locus_tag="VF089_18370"
     CDS             6571..>6765
                     /locus_tag="VF089_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175982.1"
                     /translation="MKIPVDQITQSPKEIVFSEKIEELNSLFANAKYRDFHFPSELDV
                     NLVYFRSGQELFFQGSFDGRF"
BASE COUNT         1613 a         1693 c         1839 g         1620 t
ORIGIN      
        1 aattgttgcg gcgcggcaag ttcgttgaaa cgcttctagc taatatcacc gcaggagtga
       61 ttgccgtcga tccgagcggg aggataactt cttggaataa ggcagccgaa cagatgttag
      121 ccgtagatcg ttcggctgct ctcggaaaaa actacaggga agcgttcgaa aacgaaccgc
      181 tggtcgtcat gcgcgacttg atcgacaacc tgaaagatcg cgaaagcagc gaagccgaat
      241 tgcagataca gctgccggac catctcgtta cggtatttgc gaccgccgct accctcagag
      301 atgatgccaa tgaaagcttg ggtatcatga tttttctcga ggacatcacc caaattcaaa
      361 aagtacagag aatggaagca tggcgcgagg tcgcccggcg catcgcccac gaaattaaaa
      421 atccgctcac gccgatacag ctctcagccg agcgactgcg caaacgctat gctaaactgc
      481 tgcaagggga cggcgcggta ctggacaagt gcacgacgac gatcatccag caagttctcg
      541 agctcaaaaa cctggtgaat gagttttcgc aattcgcgag gctccctacc gctcagccgg
      601 tgccgaacga cttgaatttg atcattcagg acgcactatt ccttttcaag gaaggccata
      661 accatatcga ttttgaattc cgtcaggggg acttgccatc cctggagctc gatcaggagc
      721 aaatcaaacg ggtgatgatt aatttgttag acaatgccgt ggccgccctc gctgacggcg
      781 ggcacgtcca aatatcgact gcctacgatc acgctcggaa tacagtttac cttgaagttg
      841 ccgacgatgg ttgcggtatc gcgccggaag cgcgagttcg aatattcgag cctggatttt
      901 ccactaagaa acagggtacc ggcctggggc taaccatcgt caatcaaatt gtcgaagatc
      961 atcgtggcta tattcgggtg cggaacaacg agccgcacgg caccaggatg atcatagagt
     1021 ttccggtgcg ggaacaggac gcgctacagg aatctaaaag aaaagctgcg cagctgtaaa
     1081 atggaggggc agtgaacgag ttatcatccc agaaaaccat ccttgttgtc gatgatgagg
     1141 acaaggttcg ggagtcggta aaagaagtgc tgacggacga ggggtatcgg gtactcgata
     1201 ccgccgatgc cacttgtgtt ctcgaaatga tcgaagcgga gcaacctggc cttgtgctgc
     1261 tcgacatttg gatgccggaa attgatggta tcggcttgct caaggagatc aagagcaaga
     1321 agccttcgac caatgttgtg atgatctccg ggcacggcaa tattcacacg gcggtgaccg
     1381 cgaccaaatt tggcgctttc gattttatcg aaaagccggt gtcgctcgat ggcctgctcc
     1441 ataccgtcca acgcgcgcta ggcgagctct gtgttactcc gagcctcgcc gaagtgggat
     1501 atccagccgg ggacatcccg gactcgctga gtacggcccg gcagattgtc acatccgccg
     1561 aaccgatgat ccgacaaagg accttaagta aaagcgtagt gagttcaggt caaggattgc
     1621 attcaggaat taaaacaggg ttgattcttc atcctctccc accaaatagc gggatcttat
     1681 tcgagggaat ctcgggtgat gttagcgtac ccgctcattt agattatgtt ggatcgacgg
     1741 ggtttgccac atcattgagc catcgtggtt ttacggttgc gaccgtagag cattttttgg
     1801 cggtcctcca tagttatggg attacaaact tgctggtgaa agttcaaggc gaggttccga
     1861 tcttggatgg atcggcggcg gagttttgtg aactcatcga ggaagctggg ctgcacgagc
     1921 aagacgaaag ctggtccagg atcgtcatcg atcggactta tcgtgtcggc gaagacggag
     1981 gagagtggat cagcatcgaa ccggcggaga cgttctcagt ccactatgtt ctgaagtatc
     2041 cgaagccggt ggggatgcaa gaatacatgt ttactcatca tagctcgagt tcgttcaaag
     2101 aagaaattgc gccggcaaga accttcggct ttttgcgcga tatggagaaa atgcagaagc
     2161 tcggcttgat caacggaggt aggcttagta attgtatctt gatcgatgac gagaagatcg
     2221 taaatactga tttgcgtttt ccggaggaat tggcgcggca taaaatcctt gacatcatcg
     2281 gggacttcta tctgctcggg agacccatcc gcggcaaaat cacggctcac atgactgggc
     2341 attccgacaa cattgcgtta cttaaggctg ttcggactgg catggagcta taggctcatc
     2401 cccttaagtg ctgacggctt gagttgatcg gtatggattc atcttcgcct tattccagac
     2461 tgttcacgat caacgaagcg aacgcgcttt tgcccatagt gcgtccgacg atcgaacgga
     2521 ttctggagat catttgccgt ttacgggtcc agagcgaaat agtgattcgc gcggaacaga
     2581 tcgacccgga aacgcctaac ctgatgcgcc gtttacagca gaacggcgag atcgccgacc
     2641 tgatcaagca gatcaacgac gctgtcaatg aaattcagca acatggctgt gtgtgcaaag
     2701 gcgtggaaca aggactgctc gattttccct gcctattggg agaagaaatc gtgtttctct
     2761 gttggcaata cggagaacct gagattacct actggcataa aatcgaggac ggctttgccg
     2821 ggagacgccc attgttggac tccactgaca ccggttcgag tggtggccgt gccgtgcact
     2881 gagtctcgca agcgggtgct ctcctgaagt ttccgcatta atgtcattcc atagcttttc
     2941 tggctccgta tgatttctaa ctctgagccg aacttgtccg aactgtccca gcgcatcaag
     3001 aatgaagggc aacggcttgg ctttgaactc gtcggcattt ctccggtgcg ggtgccgccg
     3061 cacgaggagt cgttcgctcg ttggctacgg aaaaaatacc acggtcatct ggactatatg
     3121 gagcgaaccg agcttttgcg gcgcgatcca aggctgcttg ttccctgggc cgtgtcaatt
     3181 atttccgtag gaatgaacta ctacacgccg cttaaacgag taactccagc cgagcgcggg
     3241 cgcggctgga tctcgcgcta tgcctggggt gatgattatc atgaaatcat ccgtgagaaa
     3301 ctggaagccc tgcttacttt tcttcgtgag attggcggcg acacggtgga aggtagggcc
     3361 ttcgtcgatt ccgccccgat tctcgagcgg gactttgcgg gtactgccgg catcggatgg
     3421 attggcaaaa acacgcagct gatttcgcca aaaaaaggat cctggttttt tcttggtgaa
     3481 ctgtttacca atatccccct ttcttacgat caagcgattc gtgatcgctg cggtcaatgt
     3541 gatctgtgtc taaaagcctg cccaactggt gccttcacgg ggccgtatct gctggatgcg
     3601 cgtcgctgca tctcctacct tactatcgaa ctcaagggtt ttattccgcg tcatttgaga
     3661 ccacttatcg gcaatcatat ttttggctgc gacatctgcc aggatgtctg tccttataat
     3721 gttaaagcga gttccacggt cgaaccctct tacctgccgc gccaagggct ttacgcgccc
     3781 gaattgatag gactgctgtc gatcggccga gaggagtttc gccggcgctt tcgtggcagt
     3841 cccattctac gcgcaaaacg acgcggtttt ttgcgcaatg tagcggtagc gcttggcaat
     3901 aatgctgggc cggaagcggt accggcactg acccgtgcac taaatgacga ggagtcatta
     3961 gtacgaggtc atgttgcatg ggcgctcggc cggattggta cctctgaagc gctcgcggcc
     4021 ttgcggcaga aattgctgtc tgaagataac ccggaggtca aaagcgagat tcaagaggct
     4081 cttggtgagg ccactgaacc tttggctaat cactcgtaaa tgcgtcgttg tagcaagcct
     4141 ctttcaagag gtgttttcgg ctgggcaaag catgacctcg agcgccctca gcaggtcatg
     4201 ctgcattctc atgattctgc cctgaccatc gacacaggcg tgcttggtcg agccagaggc
     4261 aagcagcaga gcttccgcct cacgcgtaat tcggtaggta aatgttagtg atgctcggct
     4321 aaccgaagtt atgcgggtct caatgacaat caggtcatcg taatgcgctg gtctgaggta
     4381 acggcatgac agctccgcca ctggaaaatg catgccgcgt cgctcgatgg ttgcgtacgg
     4441 aatcccgcat gcgcgcagca attcagcgcg gcctctttca aaccagcgta gatagttggc
     4501 gtagtaaacc acgcccatct gatctgtatc gccatagatg acccgatagg ttgtcacatt
     4561 ggaagtgctt ggtttgctcg aactcatacg ctctcactcg tatgttgttt tggagctttt
     4621 ctcaacgcta tagatctcga tccgccttga caacctgaaa tgaagtatgc tttaaggtct
     4681 aaaatttttg gacgatttta ttcgtgattg ccggtacgtg atttgccggt aaaggttttc
     4741 ggaggatgga aatgaagcga aaaggtggat tcttgacgtt atcagccggc tcgctcctgc
     4801 tcgcggtggg tttactcgcg tgttcatctg ttcaggaaga aacggccgct cccgcgcaaa
     4861 agtccccatc ggcgcaggcc aaacctgcgg gcgaagcaaa gccggccaca gagacaaaag
     4921 ccacaaagag tgaaacaaaa caaaccaagt cgaaagcagt cgaagttgaa ggtgtgatca
     4981 gcagtgtcgg ccagcgcacg attacctttc aagtggagcg aaagggaaag gttcgcgagg
     5041 aggtggttgg agtcgatgac aaaactaaca ttgaaaaagc cggcagcaga gtcaagttaa
     5101 aagatttgca ggaaactgac aaggcgttga tcaactatga acccgaagcc tatactccgg
     5161 caaccgccat caaggtgatt ggacaaggta aattacagaa ggtcggcggc ggcgattagc
     5221 tgccgtagcg actgcggcgg tcagcgccga ccaagtttag ctcgatgcta gtttcgtttc
     5281 ttaagacacg cagagagatg cgttctcccg gtcgcttctt ccaaagctcc tgatagagct
     5341 gccgccgaga tcggatttgc gtactctcaa catccagaat gatatctcct tctttcaatc
     5401 catgacgatc agccgggctc ccttccgtca ctccgccgac aaccacatgt ccggctagca
     5461 agtgcggata gaaacctaac caaggctttt gactgatcag agaacgttgc ttgaacgcct
     5521 gctcctgatc gaggtagtaa ctgatcggaa tggccaatga gaattttcca atgtctggaa
     5581 gattcaggga aaccactccc agcatctctc ctttgaaatt tgccaatgtg ccgccaccga
     5641 agcctggatt gacggctgtc actctcaccg tttgatcgag catgtactcc caatggccgt
     5701 cgtagggctc cagtgaagtt acatagcctc cactcacttt tcgttcgttg tttccgctgc
     5761 tggcgacgat caggctagcc tggccaagta ccgccggttc tgttgacgct ttgaggaaag
     5821 gcagccctcg tgccgggatc ttcaaaagcg ccagcccgct atcgagatcc tgtgtgacga
     5881 tttcacccgg gtactcttct ccctcggcca cagtgacgtc aatcgatgaa gctccggtca
     5941 caacgtaatt gaccgttaaa atgtaccctt ccggatcgac gatcgttccg gatccggtgc
     6001 gctccgaacc caggttcttg gttgatggat gatcctccgg gatggacgcg tggatgtcta
     6061 ctgttgccgg aatgattgca tggatcaagc cgagatggcc gttcaaagtc ctgctccttc
     6121 ttcgacattg ctcgctgccg ctaggcacga gcctatctcc cgtattattc tacgcggctc
     6181 acgcgggtct aaagcttcct tctgaattct attgatgatt gagaggttgt caacggtcat
     6241 tggcggcttg cgcgcttctt cgttgcggta cgtcaacgat caacctttat atgcgcacga
     6301 gttgcgccgg ttgacaaagg gcgaaaggta atttcggaac tgatgatgcg gattggacgc
     6361 gccgcctctt tagcggtgag tgccggacaa tcccgcataa gggcaaatag cggtagcgct
     6421 ttttacactg gctgctttga agatgaaagg acgcaaggct cggaagtggc ggataaacca
     6481 gagacttttg catctctgga agatcccgca gcttgacttt ggaggagaat ccaatatatt
     6541 cacatggtcc ggccaaccag atcgttcgct atgaagattc ccgtcgatca gataactcag
     6601 tctcccaaag aaattgtttt ttcggagaag attgaggaat taaactcgtt attcgcaaat
     6661 gcgaagtatc gggattttca ctttccatcc gaactggatg taaatctggt ttacttcagg
     6721 tctgggcagg agcttttctt tcagggatcc ttcgacggca gattc
//
LOCUS       DASUTG010000793         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1157311, whole genome shotgun sequence.
ACCESSION   DASUTG010000793 DASUTG010000000
VERSION     DASUTG010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157311"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF089_18375"
     CDS             complement(<1..253)
                     /locus_tag="VF089_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6175983.1"
                     /translation="MHYRDLRGWLEGVESFGELRVVEGADWNQEIGAVAEVAARSETP
                     PAVLFDKIIGYPAGRRVLTGIHNPTLKRQCLTNHLPLDYT"
     gene            453..1733
                     /locus_tag="VF089_18380"
     CDS             453..1733
                     /locus_tag="VF089_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX6175984.1"
                     /translation="MRLKQLSLISLALIGMLILSCSSETRSQSSSRQYAPGTGPADRL
                     QLPPPFATPSARNTSKVIGWPPGKTPTAAPGFAVSLFADQLSNPREAYVLPNKDILIV
                     EAARQFPGRPAKSANQITLFRDSDNDGKPEVREVFLTGLNMPHGMLLLGNWFYVGNTD
                     GVVRYPYRAGQTKIEGQGEKILDLPAGGHYTRHLIADPAGKKIYVAVGSSSNVDEDGR
                     DANDPRRAAILEMNPTGSGMHVYASGLRNPVGMDWEPKTKTLWTVVNERDHLGDDLVP
                     DYLTSVKPGAFYGWPYSYFGQHEDPRKKGQRPDLVAKAVKPDYALGAHVAALGLAFYD
                     GKSFPARYHGGAFIGMHGSWNRSKMVGYKVVFVPFDKGDPAGPMEDFLTGFIADESKF
                     EAYGRPVGVTVAPDGALLVADDAGGKVWRVSAKK"
     gene            2000..3094
                     /locus_tag="VF089_18385"
     CDS             2000..3094
                     /locus_tag="VF089_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6175985.1"
                     /translation="MSTKCRAAILVEPNQPLIIDEVEFPDPAPDQVLIKLFASGVCHS
                     QIHTMRRPARSADRLPALLGHESTGVVAARGRDVRHVKEGDHVITTWVDRDNSTTDQP
                     LVAHALNDRAQSIASWQGKQVSHSAATWAEYAVAQERMVVGLPKDVPTDVTAIIGCAV
                     MTGAGAIINTLQVRPGQSVAVFGAGGIGLCAIAAATALDAHPVIAVDVSEDKLAFARR
                     FGASDLINASEADPVQTIRDLTNGGVDYAIDAIGLPRTQEQILQATKMGFPGFHRGGT
                     ALLIGITPPGSQAVLDTSLFIGSRSFTRTSGGDCRPDRDFPLFVRWYREGKLKLEELV
                     TRRYPLEQINEAVDDLEQGRILGRAIITYN"
     gene            complement(3278..3832)
                     /locus_tag="VF089_18390"
     CDS             complement(3278..3832)
                     /locus_tag="VF089_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX6175986.1"
                     /translation="MAKWFFEPGHTAAEFSVRHMMVTFVRGHFKDIHGTLVFDPADPG
                     ATSVEVTIEAAGIWTGEAARDAHLKSEDFLDVEKHPKITFAGKQVELAGADEAVLTGE
                     LTIRGITRAVPLRVHYLGQWQTPWWEDGVDKGPKSRAGFAATTTINRHDFGVSWNSAL
                     DRGGVVVGNRVEITVDGEAILQEG"
     gene            3995..>4179
                     /locus_tag="VF089_18395"
     CDS             3995..>4179
                     /locus_tag="VF089_18395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6175987.1"
                     /translation="MDSRPGIAEHQRPRFDYLHHISLPCRDVEESKRFYTQVLGGELI
                     HDISGFAEVRIADIISG"
BASE COUNT          936 a         1241 c         1160 g          842 t
ORIGIN      
        1 gcgtgtagtc gagcggcagg tgattggtca ggcactgccg cttaagggtc ggattgtgaa
       61 tccccgtcag cacgcggcgg ccagccggat agccgatgat tttatcaaac agcaccgccg
      121 gcggcgtctc tgaacgcgcc gccacctcgg ccaccgcgcc gatttcttga ttccagtctg
      181 cgccttccac gactctaagc tcgccgaatg attccacgcc ctctaaccag ccacgcaaat
      241 cgcgataatg catttagccc tccgaagttg gcgacgatct tagaacggac agaacgccgc
      301 tacaaccgag cacggaattc tattcgccgg atgcactccg atgccgaacc aaagaaaggt
      361 taatcccact tcacagcaaa tcgcggattg tctatggtct aaacagtagc gtcgcagcag
      421 gcagccactc gtcctatgca aaggagaatt ccatgcgcct taaacaactg tccctgataa
      481 gccttgcgtt gatagggatg ttgattctct cgtgttcgag cgaaactcgc tctcaatcat
      541 ccagcaggca atatgcgccc ggcacgggac cggcagacag gctgcaattg ccgcctccat
      601 ttgcaacgcc ttccgcacgc aacacctcaa aagtgatcgg ctggccgcct ggcaagacgc
      661 cgacagccgc gcctggcttt gctgtcagcc tgtttgccga ccagctcagt aatccgcgcg
      721 aggcttatgt gctgcccaac aaagacattc tcattgtcga agccgctcgc cagtttcctg
      781 gccgtccagc caagtcggcg aaccagatca ccctgttccg cgacagcgat aatgatggca
      841 agccggaggt tcgtgaagtt ttcttgaccg gcttgaatat gccgcatggt atgctgctgc
      901 tcggcaattg gttctacgtc ggcaacaccg atggagtggt gcgctatccg taccgggccg
      961 gacagaccaa gatcgagggt caaggtgaga agatcctcga tttgcccgca ggaggacact
     1021 acacgcgcca cttgatcgcc gatcccgccg gcaaaaagat ttacgtcgcg gtgggctcca
     1081 gctctaacgt cgatgaagac ggcagagacg ccaacgatcc acgccgtgcg gcgatcttgg
     1141 agatgaatcc cactggcagc ggcatgcacg tttacgccag cgggctgcgc aatcccgtgg
     1201 gaatggattg ggagccgaaa accaaaacac tgtggaccgt ggtcaacgag cgcgatcact
     1261 taggagacga cctggttccc gactatctga ccagcgtcaa acccggcgct ttctacggct
     1321 ggccttattc ctacttcggc cagcacgaag acccgcgcaa gaaaggccaa cgaccggacc
     1381 tggttgccaa agcggtaaaa cccgactacg cgttgggcgc ccacgtcgcc gcgctcgggt
     1441 tggcttttta cgacggcaag tcttttcccg cgcgctatca cggcggcgcc ttcatcggca
     1501 tgcacggttc atggaatcgc tccaagatgg tcggctacaa agtcgtgttt gtcccgttcg
     1561 acaagggcga ccccgccggc ccgatggaag atttcctcac cggcttcatt gcggatgaga
     1621 gcaagttcga agcttacggc cgcccggtcg gcgtcacggt agcgcctgat ggcgcactgc
     1681 tggttgctga tgatgccggc ggaaaagtgt ggcgggtcag cgcaaaaaag tgaaccggat
     1741 ctgcaggatc gaaaaatgat cggcaacagc cttgtcttgt caaagcgtcc ggagcgctgc
     1801 tatgactcct ccggtttgga agtatggaaa gccaccgata agaacgaact acatcgaaca
     1861 tgactgcggc tcgaggggat ggtatgagtc tgtagtggga cgcccaggag aagagttaag
     1921 ggaagtagtc gagtactgcg ctattcacga gatagaaagc gacagctcgt gatcgagcgg
     1981 cacgtgaatg gagaaaagca tgagcacaaa gtgccgggct gcgattctgg tcgagcccaa
     2041 ccagcctctg atcatcgatg aggtcgagtt tccggatccc gcgccggatc aggtgctgat
     2101 caagcttttt gccagcggtg tttgtcattc gcagattcac acgatgcgcc ggccggctcg
     2161 atccgctgat cgcttgccgg cgctgttagg ccatgagtcg acgggagtcg tggccgcgcg
     2221 cgggcgcgat gtgcgccatg tgaaggaagg cgatcatgtc atcacgacct gggtcgatcg
     2281 cgataattcc acgacggatc agccgctggt agcgcacgcg ctgaacgacc gggcgcagtc
     2341 aattgcttcc tggcaaggca aacaggtgtc gcattccgcc gcgacctggg cggaatacgc
     2401 tgtggcgcaa gaacgcatgg ttgtcggtct gcccaaagat gtgcccaccg acgttacggc
     2461 gatcatcggc tgcgcggtta tgaccggggc cggagcgatc atcaacacct tgcaagtgag
     2521 accgggacaa tcggtggctg tcttcggagc cggaggcatc gggctttgcg cgattgccgc
     2581 ggcgactgcg ctcgatgccc accctgtcat cgccgtggat gtaagcgaag acaagctcgc
     2641 gtttgcacga cgtttcggcg ctagtgattt gatcaatgcg agcgaggccg acccggttca
     2701 aacgattcgg gatctcacca acggcggagt cgactacgcc atcgacgcca tcggtttgcc
     2761 acgaacacaa gagcaaatcc tccaggccac aaaaatgggc tttcccggtt ttcatcgagg
     2821 agggaccgct ttgctcatcg gcatcacccc gcccggctcg caagcggtcc tggataccag
     2881 cctgttcatc ggcagccgga gctttacccg caccagcggc ggcgactgcc gcccggatcg
     2941 cgatttcccg ctgttcgtcc gctggtaccg agaaggcaaa ctgaagctag aagaattggt
     3001 cacacggcga tatccgctcg agcagatcaa tgaagcagtt gatgatctgg aacaaggccg
     3061 gatcctcgga cgcgcaatta tcacgtacaa ttagacccgt cgacgaatcg aatgccacgt
     3121 ctttgattca cacgatcttc aggacaacac cgactctaat aagaatgaag cgaagattcc
     3181 gctgatcctg ctgcctcatt atgcaggcat aagccgcaca tccaataaag aagaagcttt
     3241 ggtggtagca gttccggtcc gatgaagctg tgcaacatca accttcttgc aggatcgctt
     3301 cgccgtcaac ggtgatctca accctgttgc cgacgacgac cccacccctg tctaaagcgc
     3361 tgttccagct cacgccgaaa tcgtgacgat tgatcgtagt cgtggcggca aaccccgccc
     3421 tgctcttcgg tcccttatcc accccatctt cccaccatgg ggtttgccac tgacctagat
     3481 aatggacgcg cagcggcacc gcgcgggtga taccccgaat cgtcaactcg cccgtgagaa
     3541 ccgcttcatc cgcgccggcc aattcaacct gcttccctgc gaaagtgatc ttcggatgtt
     3601 tctctacgtc gagaaagtcc tcgctcttaa gatgagcgtc gcgcgccgct tcgcctgtcc
     3661 agattccagc agcttcgatc gtgacctcga cggaagtcgc gccgggatcc gccggatcga
     3721 aaacaagcgt gccatggata tctttgaaat ggccccggac aaaagttacc atcatgtgac
     3781 gcacagaaaa ctccgccgcc gtatgccccg gttcaaaaaa ccatttggcc atagtccgtt
     3841 cccttcgcag caaagatatt ctctgagaat catttttacc ttaacgctca gcgcactacc
     3901 agaagcagct ttggcagtac agaatagtcg aatcttgttc cgttagcaca acccgtgtaa
     3961 tagtccggcc atcacactcc ggaggagccg agccatggac agcagacccg gaattgcgga
     4021 gcatcaacga ccccgcttcg attacttgca ccatatcagt ctaccgtgcc gcgacgtgga
     4081 agagtcaaaa agattctata ctcaggtgtt gggcggggaa ctcatccatg acatttccgg
     4141 gttcgctgag gtgcgcattg ccgatattat ctccggcat
//
LOCUS       DASUTG010000794         6299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_565881, whole genome shotgun sequence.
ACCESSION   DASUTG010000794 DASUTG010000000
VERSION     DASUTG010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565881"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1805
                     /locus_tag="VF089_18400"
     CDS             60..1805
                     /locus_tag="VF089_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6175988.1"
                     /translation="MTTAADVLIDAIHDWGVDVIFGLPGDGINGIMEALRKRQEQIQF
                     IQARHEEAAAFMACAYAKYTGKLGVCLATSGPGGIHLLNGLYDAKLDGQPVLAITGHH
                     YHDLIDTHAQQDVDLDRVFMDVSVYNTRIMGPAHVEQVTHLACRTALSYRGVAHINFP
                     VDLQEHEIKEHSKRNIPGHTSRVFATKAGLPEEKDLRAAAEVLNAGKKIAIMAGQGAL
                     AATEELEELAGTLAAPIIKALLGKAAVPDDSPFTTGGIGLLGTKPSQEALEECDTLLM
                     IGTSFPYIEFLPKPGQARGVQIDINGARIGLRYPVEVGLVGDSRLTLRQLLPMLKRNQ
                     DRSFLKKAQDGMIEWWKLMEERGTRKDKPMKPQVVAWELGKRLRPDAIVSADSGTIAT
                     WFARQIRVQRGQKYSLSGTLASMGNGLPYTIAAQIAYPDRQCVGFVGDGGFSMLMAEF
                     ATCVKYRLPVKIIVIKNNTLGMIKWEQMVFLGNPEYGCELQPIDFAAFARACGGTGFT
                     IEEPASCGAILDEALALPGPVIIDAVVDPFVPPMPPKITFAQASNFAESLARGEPNRS
                     KIAWTVLSDKVRELI"
     gene            1819..2025
                     /locus_tag="VF089_18405"
     CDS             1819..2025
                     /locus_tag="VF089_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6175989.1"
                     /translation="MPRIIIDRDELKQRVNDGAHLVEVLPAKEFARAHLPGAINIPLA
                     KFSEKTVADLDKKNATIVHCYDYQ"
     gene            2122..2544
                     /locus_tag="VF089_18410"
     CDS             2122..2544
                     /locus_tag="VF089_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6175990.1"
                     /translation="MPAVREHREPESLWVMDHMKVELPTCRIDEPVGQAGARAQERGY
                     SLCPVVNQNGIVLGLIGKGDWDSDPTASAEQLMDSAPTTLRPSDPLEKAKQMLNESNR
                     GAVLVTNSDGRLLGAFFGVSEKEQPGRKQQLPNSEVWS"
     gene            2911..3453
                     /locus_tag="VF089_18415"
     CDS             2911..3453
                     /locus_tag="VF089_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX6175991.1"
                     /translation="MNLELAGNGKLTPQQLRQELQQEQTSDLKRRRGIIACSLVGMAG
                     MTAVTLLQTGIIKHLPDPPFGNFDSDRVNSSDTAYRLGAPDGSLSLASLAINIPIAAF
                     GGADRGQTKPWVPLIAALKATGEALAASWYFYQMPAKEKAWCGYCILGALANIGIAAL
                     TLPEARKALAVIMSETTKAP"
     gene            3470..3640
                     /locus_tag="VF089_18420"
     CDS             3470..3640
                     /locus_tag="VF089_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6175992.1"
                     /translation="MKRPVEPDPRPERKNVDIERRGDNNESEERPRRQNRGRRGSGPE
                     REPSTRKDRGRS"
     gene            3772..4506
                     /locus_tag="VF089_18425"
     CDS             3772..4506
                     /locus_tag="VF089_18425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX6175993.1"
                     /translation="MCPLISIIIPARNDAVALQHTLERLQQLTGVGGVEILVAASGDK
                     RSTEQAAAGRAQLLWPAGSSRSALMNSAADIARGEILFFLHADSLPPRDALPRIRSLL
                     QDPKVLGGAFEHRFLESSWSLRAISWINRRRYWLTGNYYGDQGIFVRASVFRDLGGYR
                     DLDLMEDLDLSQRLKRRGRTALIREPLLTSGRRFIDRGPWRTFVFIVWLLFLHTMRFD
                     TQRYARRWRGPAGAVPGSRWIGIRGR"
     gene            4496..5653
                     /locus_tag="VF089_18430"
     CDS             4496..5653
                     /locus_tag="VF089_18430"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6175994.1"
                     /translation="MEGKTMTTAGHEGAILITGATGFIGRHVARRLYQTGSYVMALAR
                     DQGGITARRRVEKALQISDSNDRLEVIHGDLSQPGAGLCKSDVAGLRRTVQTVIHCAG
                     DTSFFPQSMSASRVLSIDGPLALLAALGAGGLERWCQISTAFVCGRRCGTVFESEGVV
                     GQEFHNPYEQVKLESEIALRDVCSRLEVDLRILRPSVVIGPESSTQGGSPSSLLYQFI
                     RLAAALAGCGKGAELPVRIQGRPPAQFNIVPLEYVSAAIAAVANQSGTNGGTFHLVAS
                     SAPTQQEVLQMITSRLGVTRLRLIDAHCEPLEHPSPLEKKLSRMLAPYHDYLVQDIRF
                     SDTAALSLLKPLGITCPRIDQGIMDRFIDTALAHPARGCHANGNSHVSFCP"
     gene            5781..>6299
                     /locus_tag="VF089_18435"
     CDS             5781..>6299
                     /locus_tag="VF089_18435"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6175995.1"
                     /translation="MMLPCFAPYTFRRRISQKHRRWQGSSDVILWLDTFNNHFHPETA
                     EAALEVLQAAGYRVGIPRLALCCGRPLYDFGFLDTAKRLLREILDHLQPELESGTPIV
                     VLEPSCAAVFRDELVNLFPEDANAKRLCQQTFLLSEFLDKNENTSVSQIVGKAIVQGH
                     CHQSRLWGWRARP"
BASE COUNT         1349 a         1746 c         1882 g         1322 t
ORIGIN      
        1 ggctcgctgc cggcagggtc aaaattcttg ccgtttcgga tcagttgtat ggagggatca
       61 tgaccaccgc tgccgatgtt cttatcgacg ccattcatga ctggggagtc gatgtcattt
      121 tcggcctgcc cggggatggc atcaacggca tcatggaggc gttgcgcaag cggcaggagc
      181 agatccagtt tattcaggcg cggcacgaag aagcggccgc attcatggcc tgcgcttatg
      241 ccaaatatac cggcaagctc ggcgtctgct tggcgacttc gggcccgggc gggatccatc
      301 tactgaatgg actctacgac gccaagctcg atggccagcc ggtgctggcg atcacggggc
      361 atcactatca tgacctgatc gatacccatg cgcagcagga cgtcgacctc gaccgtgtct
      421 tcatggatgt ctccgtctac aacacccgga tcatgggacc ggcgcatgtc gagcaggtga
      481 ctcatctggc gtgccgcacg gcgctcagtt atcgcggcgt cgcgcatatc aacttcccgg
      541 tcgatctgca ggagcacgag atcaaggagc actcgaagcg caacattccc ggccacacct
      601 cgagagtatt cgccaccaaa gccgggttgc cggaagaaaa agatctgcgc gccgcagccg
      661 aggttctcaa cgccgggaaa aaaatcgcga tcatggcggg acagggtgcg ctggccgcaa
      721 ccgaggagct ggaagaattg gcgggcacct tggcggcacc gatcatcaaa gcgctcttgg
      781 gcaaagcggc ggttcccgac gatagcccgt ttaccactgg cgggatcggc ctgctcggca
      841 ctaaaccgtc gcaggaggcg ttagaagaat gcgacacgtt gctgatgatt ggcacgtcgt
      901 ttccgtatat cgagttccta cccaagccgg ggcaagcgcg cggggtgcag atcgacatca
      961 atggagcgcg catcgggctg cgttatccag tcgaggtggg gctggtggga gacagccgtc
     1021 tcaccttgag gcagctatta ccgatgctca agcgcaacca agaccggagc tttctcaaga
     1081 aagcccaaga tgggatgatc gagtggtgga agctaatgga agagcggggc acgcgcaagg
     1141 ataagccgat gaagcctcag gtggtcgcct gggagctggg caagagatta aggcccgatg
     1201 ccattgtctc tgccgattca ggaaccatcg caacctggtt tgcgcgccag attcgtgtcc
     1261 agcgcggcca gaagtattcg ttgtcgggca cgctcgcgag catgggcaat ggcctgcctt
     1321 acaccatagc cgcgcagatc gcttatcccg accgacaatg cgtcggcttt gtcggcgatg
     1381 gcggcttttc catgctgatg gccgagttcg ctacctgcgt gaagtatcgg ctgccggtca
     1441 aaatcatcgt gatcaagaat aatactttgg gcatgatcaa atgggagcag atggtttttt
     1501 tgggcaatcc tgaatacggt tgcgagctgc agccgatcga tttcgccgcg ttcgcgcgcg
     1561 cctgcggtgg caccggcttt accatcgaag agccggcgtc gtgcggcgct attcttgacg
     1621 aagctctggc gcttccggga cccgtcatta tcgatgcggt ggtggatccg ttcgtgccgc
     1681 cgatgccgcc gaaaatcact ttcgctcagg cgagtaattt tgccgaatcc ctggcgcgcg
     1741 gcgaacctaa ccgatccaag atcgcctgga cggttctatc ggacaaagtg cgcgagctga
     1801 tttgaacggg gagagccaat gccgcggatc atcatagacc gcgacgagtt aaaacagcga
     1861 gtcaatgacg gggcgcatct cgtcgaagtc ttgccggcca aggagttcgc ccgggcgcat
     1921 ctgccggggg cgatcaacat cccactggca aagttcagcg agaaaaccgt cgcggacctc
     1981 gataagaaaa acgccacaat cgttcactgc tatgactacc agtgagacct ggctgctaga
     2041 gccgcgtcgc ggctcgagag tttcggtttt gaaaaagctt tttatttcaa accgagaaag
     2101 gccgattggc tggcgcatgg attgccggcg gttcgagagc atcgagagcc ggaatctctc
     2161 tgggtcatgg accacatgaa ggtcgaactg ccgacctgcc ggatcgacga acccgttggc
     2221 caggccggcg cgcgagcgca ggagcgcggc tactctttgt gtccagtggt gaaccaaaat
     2281 ggaatcgttc tcggcctgat cggtaaaggc gattgggaca gcgatccgac cgcatcggcg
     2341 gaacagctta tggattccgc gccgactacc ctgcgtccca gcgatcctct ggaaaaagcc
     2401 aaacagatgc ttaatgaaag caatcgaggc gctgttcttg tcacaaattc agacggaaga
     2461 ctgctgggcg ccttcttcgg cgtgagtgaa aaagaacagc ccggccgcaa acagcagctg
     2521 ccgaattcag aagtctggtc ttgatcaagg ctgtgatgca gttgttttca tcaagcaaac
     2581 gatcaccaaa cgtcaggtgc aacgcagtca aaaatgcgca ggaaggcagt ttctcttgaa
     2641 acgtgcgtcg cgagctggat ggattaatgg gttactcgtt gtcggtgaat tctgtctgtt
     2701 gctgtggctc gagcaccgcc ggcccgttgc gccggtccgt tgaaccatgc cattcgcaag
     2761 ccagccgccc agctggcaac ttccgggaga cggtgaaccc cgacgacagg ctttgccggg
     2821 agccgcgaac tgcttggcgc agtagtaacg gagacactct tgatatggaa ctcgggaaac
     2881 aaagcttgaa ttctagattg gagctttgct atgaatcttg aactcgccgg taacggaaaa
     2941 cttactccgc agcagctacg tcaagaactc cagcaagagc agacctctga tctgaagcgg
     3001 cgccgcggta ttatagcttg ctcgctggtc ggtatggcag gcatgaccgc ggtgactttg
     3061 ttgcagaccg gcatcatcaa gcatcttccc gatccgccgt ttggcaactt cgattccgat
     3121 cgagtgaact cgtcagatac ggcttatcga ttgggagcgc cggacggctc gttatcgctg
     3181 gcgagtctgg ccattaacat tccgattgcg gcatttggcg gcgccgatcg cgggcaaaca
     3241 aaaccttggg ttcccttaat cgccgcactg aaagcgacgg gtgaggcgtt ggcggccagt
     3301 tggtattttt atcaaatgcc ggcgaaggaa aaggcctggt gcggctactg catcctcggc
     3361 gcactggcaa acatcggcat cgcggcgctg accttaccgg aagcgcgaaa ggccttggct
     3421 gttatcatga gtgaaactac aaaagcgccg tgatcggacg gagggagaga tgaaaagacc
     3481 cgtggagcca gacccacgac ctgaacggaa aaatgtcgat atcgaacgcc gaggcgataa
     3541 caatgagagt gaagagcgcc cgcgccggca aaatcgcggc aggcgcgggt cggggccgga
     3601 gcgcgagccc tcgacgcgaa aagatcgcgg tcgatcctaa ttgaagttgt agatcggccg
     3661 agcgattcat tccagatctt gtcggcacgg atggcccggg aggctctccc tcgcgaacgt
     3721 catgagcacg aagactcagg cgcttaaagc tggctttcac ctcacgtaag aatgtgcccg
     3781 ctgatctcga tcatcattcc cgcgcgcaat gatgcggtcg ccttgcagca cacgcttgag
     3841 cgattgcagc agttgaccgg ggttggcggc gtagaaatcc tggtagcggc gtcgggcgat
     3901 aagagatcaa ccgaacaggc ggccgcgggc cgcgcgcaac tgctttggcc ggcaggttcg
     3961 agtcgttcgg cgctgatgaa ctcggccgcg gatatcgcgc gcggcgaaat cctttttttt
     4021 ctccatgcgg actctcttcc tccacgggat gccctgccgc gaatccgatc gctgctacag
     4081 gatccgaaag ttctcggcgg cgcgtttgag caccgctttc tcgaaagcag ctggagcctg
     4141 cgcgcgatca gttggattaa tcgcaggcgc tattggctta ccggcaacta ttatggagat
     4201 caaggaatct tcgtgcgcgc gagtgtcttt cgggatctcg gcggctatcg ggatctcgac
     4261 ttgatggaag acctcgacct gagccagcgc ttaaagcggc gcggccgtac ggccttgatc
     4321 cgtgagccac ttctgacgtc cgggcgacgc tttatagatc ggggtccatg gcgaacattc
     4381 gtcttcatag tttggttgct ctttctccat acgatgcgtt tcgatacgca gcgatatgcc
     4441 cggcgctggc gcggccctgc cggcgcggtt cccggcagcc gctggatagg aatacgtgga
     4501 aggtaagacc atgacgacag cgggccatga gggcgcgatc ctgatcaccg gcgccacagg
     4561 ttttatcggc cggcacgtcg cgcgccggct ttatcaaaca ggatcctatg tgatggcgtt
     4621 agcgcgcgat caaggcggta tcactgctcg ccgccgcgta gaaaaagctt tgcaaatctc
     4681 cgatagcaac gatcgcctgg aagtgattca cggtgatttg agccagccag gcgcggggct
     4741 gtgcaaatcg gacgtcgccg ggctacgccg tacagtgcaa accgttattc actgtgcggg
     4801 cgataccagc tttttcccac aatcgatgtc agcttcacgc gtgctctcga ttgacggacc
     4861 gctggccttg ctcgcggcgc tcggcgccgg aggactcgag cgctggtgtc agatctccac
     4921 agcctttgtt tgcggccggc gttgcggcac cgtgttcgaa tccgaaggcg tggtggggca
     4981 agagtttcac aatccctacg agcaggtaaa gctcgaatcc gagatcgcgt taagagacgt
     5041 ctgcagcaga ctcgaagtcg atctgcggat tctcagaccc agtgtcgtga tcggtccgga
     5101 gtcctcgacc cagggcggaa gcccgtcaag cctgctgtat cagttcatcc gtttagccgc
     5161 ggcgcttgca ggatgcggca aaggagctga acttcccgtg cgcatacaag gccggccgcc
     5221 cgctcagttc aacatcgttc cgttagaata cgtcagcgcc gcgatcgccg ccgtggccaa
     5281 tcagtccggg acaaacggcg gtacatttca cctggtggca tcgagcgcgc cgactcagca
     5341 agaggtactg caaatgatca catcccggct cggtgtcacc cgtttgagac tgatcgatgc
     5401 tcattgtgaa ccgctcgagc atccgtcgcc gctggaaaaa aagttgagtc gtatgctcgc
     5461 gccctatcac gactatctcg tgcaggacat caggttcagt gatacggctg ctctatctct
     5521 actgaagcct ctaggtatta cttgtccgag aatcgatcaa ggcattatgg atcgttttat
     5581 cgatactgct ttggcgcatc cggcgcgcgg ctgtcacgca aacggcaaca gtcacgtttc
     5641 tttttgtccc tgagctggat ttggtgagcg aatcagccgt cactccacca tatctgcaca
     5701 cgtctatacg cgctgtacca aggctgcaca ttgtcgcgtt ctaattaaga aactcactga
     5761 catttaagaa agcgaattaa atgatgctcc cgtgttttgc gccgtacacg tttcggcggc
     5821 gcattagcca aaaacatcgg aggtggcagg gcagttccga cgtaattttg tggctggata
     5881 ctttcaacaa ccattttcat ccggagaccg ccgaagcagc gcttgaagtt ctacaggctg
     5941 cgggttaccg ggtcgggata ccgcgtcttg cgctgtgctg cggccgaccg ctctacgatt
     6001 tcggattctt ggatactgcc aagcgtttgc tgcgcgagat cctggatcat ctgcaaccgg
     6061 agcttgaatc agggactccg atcgttgtcc tcgagcccag ttgcgcggct gtatttcgcg
     6121 atgagctggt caacttgttt cccgaagatg cgaacgccaa acggctttgc cagcaaacgt
     6181 ttctcttgag cgaatttctc gataaaaacg aaaatacctc cgtgtcgcag attgttggca
     6241 aagcgattgt ccagggccat tgtcatcaaa gtcgcttatg gggatggaga gcgaggccc
//
LOCUS       DASUTG010000795         9765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1602677, whole genome shotgun sequence.
ACCESSION   DASUTG010000795 DASUTG010000000
VERSION     DASUTG010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602677"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF089_18440"
     CDS             complement(<1..336)
                     /locus_tag="VF089_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6175996.1"
                     /translation="MIYDVEINSAAHYPAAEVPGLVVLAEQVGFGAFWKGESNSTDPV
                     VMLSAVASRTKTIKLGTAIYHIYGRSPVTLGIQAATLQDLSGGRLLLGLGVANKSIAG
                     WHGGVFDRPL"
     gene            complement(693..1670)
                     /locus_tag="VF089_18445"
     CDS             complement(693..1670)
                     /locus_tag="VF089_18445"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6175997.1"
                     /translation="MATLGYLEVLDPKGRVSQRFPVQSLPLTLGRAYTNQVILDDPFV
                     CPEHLTIGYDENGRLQARDVGTVNGLREIADGGPVPSLILASGTEFQIGHTRLRYCET
                     SHSVAPAEVDRPGMMRWFTSPAVGLASGFVLLVVLLVNSFFGSYERLNIARSLTEALM
                     TFSMVLVWAGLWSLVSRIVLNRFHYGQHFVLACAAIMVSLMVGTAAEWIEFIFPVSQA
                     LWVTAIVGSGFILGALVYGHLGLASSMWLRSRFWAGLAVSVAVIGLGVMVDYDNRSSF
                     STAMEYSAILKPIEPALLPATTMDRFIDSTHRLKKELDALARKARSSQP"
     gene            complement(1658..2980)
                     /locus_tag="VF089_18450"
     CDS             complement(1658..2980)
                     /locus_tag="VF089_18450"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6175998.1"
                     /translation="MRKHVIKFMLRNNRALIFWFVLSMVSLFSLSTLNSASAQAPSAS
                     APEVFRQYSEHVLRIEVVEIGSAAKASIGTGFYATQRGDIVTNYHVISKLIHAPERYR
                     VEVTNAKGQTGAVSVVGLDVVYDLAVLRGGPPSKGFLKLETARVQQGARLYSLGHPRD
                     LGLTIVEGIYNGLLQHTLYPKVHFTGSLNPGMSGGPTLLQDGKVVGVNVATEGEQISF
                     LVPAERAIALLERTAQSEIRPAASFLAEVGRQIDAYQAAYLSGMFASSQPTVALGPYS
                     LPTRPAPFFRCWADALHRKELPYVAVTHDCSTDDYMFVSSQQSSGIIRFSHHLFSTNE
                     LDPLRFFALYSAQFQAASVATTGNEEEVTPFRCETRNVRVQSGKIRAVLCVRQYVKLP
                     GLYDAVFRAATIGSRNVGLVSTLSLSGVSFENVQALTRRYMENIRWRR"
     gene            complement(3190..4395)
                     /locus_tag="VF089_18455"
     CDS             complement(3190..4395)
                     /locus_tag="VF089_18455"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX6175999.1"
                     /translation="MSIQALQHVTVLECATFVTGPYASALLANLGARVIKIESPPEGD
                     PYRYFAPDPHYSFNFAHLNRNKQSLVLDLKAAQGREICYELVKSADVFVENFRPGTAE
                     RLGLGYETLHALNPRLVYCSISAFGQSGPYVDKPGFDTLGQALSGLLSLLNDSDDPKV
                     MGMALSDYITGMSAGYGILGALLARGQSGEGCRVETSLLQATLSFIGESAAGYLRTGS
                     VPNRMARVKNAHAFAFICGDGLPLAIHCSVPEKFWLALLEAIGRMDLAADERFNTRDA
                     RRQNYACLEETLAPTFKTKSREEWLHCLEARDVPVVPLHDIAEALADPQVRHLQLIEQ
                     VEHPIAGQMEFVGAPVRYSNFSESPSLPPPLVGEHTIDVLRELGYSQRAIEELRDGHV
                     IGNTEHAPH"
     gene            4907..6394
                     /locus_tag="VF089_18460"
     CDS             4907..6394
                     /locus_tag="VF089_18460"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HEX6176000.1"
                     /translation="MRLLTLLVISSLFYFTGCHSLTVNTQINGILRVHPSSPRYFTDN
                     SGKAIYLSGEQIFVDLQDNTFNKSSIYNYQEALDFSWYMRFMRDRNLNYIRNWTLMST
                     GGASGVIASPMPYKRVSGYGNALDGEPKFDLNQFNQSFFDRMRARVIEACRNGIYLSI
                     MFYDVYAFSNFPRDPMWAGNVFNPVNNINSVNADSNGDGWGVEFFTSPSPEIKAYQQK
                     YIRKVIDTVNDLDNVFFEISNEAAGHQWHTEIIEYVKSYESSRPKQHLVFYSPGGLSG
                     AGTYDFTSYDEVVGSAADVFAGATGFGDHYRTDPPENNSGKPAIFDMDHIEAGEDLLK
                     NDHTFAWKAFTRGYHFNLYDRPFEEPQAESAAWEIARKNAGAIGNFANKKFADLSKMH
                     PQSGLSSTGYALANPGSEYLIYQSGSGSFSVNLEAGTYNYEWYNPSIASPAGSGSITV
                     SGGYQSFTPPFEGDAVLYLTGGSAGRKHNLANTHAIHDLHQNLIT"
     gene            complement(6731..7420)
                     /locus_tag="VF089_18465"
     CDS             complement(6731..7420)
                     /locus_tag="VF089_18465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176001.1"
                     /translation="MSQLRCLTIKAQEPGRLADFYRSVFELKTVSEDPQLVRLSDGVF
                     TLALLKEGKKDATGLHASGFQVEDLEEVEQKSEKVGQASPQSETQVADPDHNLIDLTT
                     KDFGVAPEKTRYRIRHIALYTADPKRLSTFYGEAFGMKEVAHSDRSSIFVSDGYFNLA
                     LLYQRVREEKNGFNHFGFHVDDLEQIRDRAEKAGTRRGAARPSRIPFAEVRLHDPEGN
                     GVDISVKGWDV"
     gene            complement(7466..8713)
                     /locus_tag="VF089_18470"
     CDS             complement(7466..8713)
                     /locus_tag="VF089_18470"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX6176002.1"
                     /translation="MPNYAAKLTFGPEAADWQERFNPDRMRRQRVERAQMLMRKYGIA
                     ALLEANHHNIRYLTALKGFAYPMCRYVLFFAEHDPVMYEHDGYYHQMPDQCPWIKEWR
                     PARSWLTGSPGPEACAVEAKAFAAEIKTELEKRGLLGEKLGLGSFDGNAREALINAGI
                     KNIVDTRQLLLEARMVKNQDEISCLKTCAAMVDGVWYRIWESLKPGMRDTDLMTIGAS
                     AGYQIGLEAAVPGGWRTGPMTFDRGCHATSRIIQVGDLVYGSYCGATYMGYGSCTYRT
                     FMVGRNPTAKEKDWYKQVRERLDAVIEEIKPGKTTADAAKHFPPASKWGYKSEVEVLA
                     SEIGHGIGLGTSTGYDAPIINRLWSFEHPQLLEEGMTIAVESREGETRVGGARLENML
                     VVTQNGAEIMDYFPRDEILVAPR"
     gene            8968..>9765
                     /locus_tag="VF089_18475"
     CDS             8968..>9765
                     /locus_tag="VF089_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176003.1"
                     /translation="MKRTSAVLTWASLLVVIAAVHPAWCAERSPEKVRIAVSSKSLGF
                     LDVWAARERGFYRKHGIEAEIIAMRPPLTIGAIQAGEIDYAFGASSISRGSMSGAPVR
                     IVSLSLKTSFHTLVARSNIKSIAEMKGKTIAVTIGAADDFVARLLVRRGGLDVRDVSF
                     VNMGGSDTRFSALATGRIDATPLSLPFFVIAKRQGYNLLGSAADVIDMATVGIGTSTR
                     KIQQEREQVKKMVRAQLDTLRWIKTQKPEVIPFLQKYFDLEEDVAAES"
BASE COUNT         2291 a         2715 c         2565 g         2194 t
ORIGIN      
        1 gagcggccgg tcgaagaccc cgccatgcca cccggcaatg ctcttattcg caacgcctag
       61 tccgagcagc aatctgccgc cggataggtc ttgcaaagtt gccgcttgga ttcccaaggt
      121 gaccgggctg cggccgtaga tgtgataaat tgctgtgcct aatttgatcg tcttagtgcg
      181 gctagccaca gccgaaagca tcaccacagg atcggtgctg ttcgattcgc ctttccagaa
      241 ggcgccgaag ccgacctgct cagccaacac gaccaagcca ggcacttcag ccgcgggata
      301 gtgcgccgct gaatttattt ccacatcgta aatcattttt ctgccccttg aaccgcttgc
      361 gcgtcagcct cgaacacgag gtacagtagt cagacgcaat cgcccaagtc aaactatgcg
      421 cagccactag tccggattgt gagacaccag aagaagaaac gggactgcgc cgataaaagg
      481 gcgagctgcc cgagaaatgg cttcaaaacg tgcacggcgt taaacccgtt taaatcccct
      541 ccttaaatcc tcccccgcga gtcgcggggg agcaaacagg tggggctgga acgattgaac
      601 taatttaatg acttgaacca tacgcaatca ctggctcatt gctttgcctt ggagggctcg
      661 ggcgttgatc gtgttacagc atgataaccg ggttacggtt gtgacgatct cgccttgcgc
      721 gcaagagcat ccagttcttt ttttaaacgg tgagtactgt cgatgaaacg atccatcgta
      781 gtcgccggca gcaacgccgg ctcgatcggt ttgagaatgg cggagtattc catggccgtg
      841 gaaaaggagc ttcggttgtc ataatcgacc ataacgccaa gcccgataac cgcgacgcta
      901 acagccagac ccgcccaaaa ccgcgagcgg agccacatcg aggatgccaa accaagatga
      961 ccgtaaacca aagcacctag aataaaacca gaaccgacga tggcagtaac ccagagtgcc
     1021 tggctaaccg gaaagataaa ctcgatccat tccgccgcgg tgccgaccat cagcgatacc
     1081 atgatggccg cacaagctag aacaaagtgt tgtccgtaat gaaagcggtt taagacgatc
     1141 ctgctcacca atgaccacaa ccctgcccag accagcacca ttgaaaatgt catcaaggct
     1201 tcggttaagc tgcgcgcaat atttaaccgt tcgtaactgc cgaaaaaaga attcaccaga
     1261 agaactacca ggagaacgaa accgcttgct agaccgactg cgggagaagt gaaccatctc
     1321 atcatccctg ggcgatcgac ttcggcgggc gctacagaat gggaggtctc gcaatagcgc
     1381 aagcgggtat gaccgatttg gaattcggtg cccgaggcga gaatcaggct cggcaccggc
     1441 ccgccgtcgg caatctcgcg cagcccattg acggtgccga catcgcgcgc ctgaagacgt
     1501 ccgttttcgt cgtaaccgat cgttagatgc tccgggcaaa caaatggatc atcgagaatg
     1561 acctgattgg tataggcacg gcctaatgtc aggggcaggg attgaaccgg aaagcgctgg
     1621 gaaacccttc ccttgggatc aagcacttca agataaccta gcgtcgccat ctgatgttct
     1681 ccatgtaacg gcgagtcagg gcttggacat tttcaaagct cacaccagat agactcaatg
     1741 tgctcaccaa gccgacgttg cgcgagccga tagtcgcggc cctgaacacc gcatcgtaca
     1801 atcccggcag cttgacgtac tgacgcacgc acagcaccgc tctgattttg ccggattgca
     1861 cgcgcacgtt tctagtctcg cagcgaaatg gcgtcacctc ttcttcattg ccggtagtcg
     1921 cgacactggc cgcttgaaat tgagccgaat agagggcgaa aaagcgcaat gggtcgagtt
     1981 cattggtgga aaataaatga tgtgagaacc ggatgatccc ggaagactgc tggcttgaaa
     2041 cgaacatgta gtcgtccgtc gagcagtcgt gagtgaccgc cacatagggt agttctttgc
     2101 ggtgcagcgc atcggcccag cagcgaaaga atggcgccgg ccgggtcggc aatgagtagg
     2161 gaccgagggc caccgtcggc tgtgagctgg cgaacatgcc ggacagataa gcggcttggt
     2221 aggcatcgat ttgccgtcct acttcggcga gaaaactcgc cgccggtcga atctctgatt
     2281 gagcggttct ctccagcaat gcgatggctc gctcagccgg aaccaggaag ctaatttgtt
     2341 ctccttcagt ggcaacattg acgccgacga ccttgccgtc ttgcagcaac gtcggcccgc
     2401 cactcatgcc ggggttaagc gagccggtga aatggacttt cggatacaac gtgtgctgca
     2461 gcagcccgtt ataaattccc tcaacgatgg tcagacctag atcgcgcggg tgtccgagcg
     2521 agtagagtcg cgcgccttgt tgaactcgag ccgtttcgag cttgagaaat cccttggacg
     2581 gtggcccgcc gcgcaggaca gcgaggtcat aaacaacgtc tagccccacc accgacaccg
     2641 cacccgtttg ccccttggcg ttggtaactt caacccggta acgctccggt gcgtgaatca
     2701 gcttggaaat aacatgataa ttggtgacga tgtcgccgcg ttgcgtagcg taaaatccgg
     2761 tgccgatcga agctttcgcc gccgaaccga tctcgacgac ttcgatccta agaacatgtt
     2821 ccgaatattg gcggaagact tccggcgccg atgccgaagg tgcttgggca gacgcggagt
     2881 tcagggtcga cagcgaaaac agagatacca tcgacaaaac aaaccagaag atcagcgcac
     2941 ggttatttct cagcataaat ttgattacgt gttttctcac tcgtacgcca agctaacgat
     3001 gcaagcatca gccgcgatcg tttgtcagta caaacccgcg cttacatctc ctcggcgatg
     3061 aaggaaagac gaaacttagc gacaagacag aaccggatgc cgcctacgcg gatgattgat
     3121 gagtacgaag tctcgtatgt taagcctatt cacgtcattc ttcgatttct tttgttgctt
     3181 cgtcatggat cagtgaggcg cgtgctcggt gtttcctatg acgtgcccgt cgcgcaattc
     3241 ttcgatcgca cgctgggaat aacctagctc gcgcaggacg tcaatcgtat gctcgcccac
     3301 gagcggcggc ggcagcgaag gagactcgct gaaattgctg tatcgcaccg gcgcgccgac
     3361 aaactccatc tgcccggcaa ttggatgctc cacctgttca atgagctgga gatgacggac
     3421 ctgcggatcg gctagcgcct cggcgatatc gtgcagcggg acgaccggaa catcgcgcgc
     3481 ttccagacaa tgcaaccatt cttccctgct cttcgttttg aatgttggcg ccagggtttc
     3541 ttcgaggcac gcataattct gccggcgcgc atctcgggta ttaaaccttt cgtcggcggc
     3601 aagatccatt cgcccgatgg cttccaacaa cgcgagccaa aatttctccg gcacggagca
     3661 atggatcgcc agcggcaacc cgtccccgca tatgaacgca aacgcgtggg catttttaac
     3721 ccgcgccatc cggttcggca cactcccggt acgcaggtag ccggcggcgc tttcgccgat
     3781 aaacgaaagt gttgcctgca gcagcgacgt ctccacccgg caaccttcgc cggattggcc
     3841 gcgcgccagc aacgcgccga gaatgccgta gccggctgac atgccggtaa tatagtccga
     3901 aagggccatg cccatcacct tgggatcgtc tgagtcgtta agcaagctca gcaacccact
     3961 caacgcttgc cccagggtat cgaaacccgg cttgtcgaca tacggcccgc tttgcccgaa
     4021 ggcggaaatc gaacagtaaa ccagccgcgg gttgagcgcg tgaagagttt cgtatcccaa
     4081 acccaaacgc tccgcggttc cagggcgaaa attttccacg aagacgtcgg cgcttttaac
     4141 aagctcgtag caaatctctc ttccttgcgc cgctttgaga tccagtacca ggctttgttt
     4201 gttgcgattt agatgcgcga aattgaaact gtaatgcgga tcgggagcaa aataacggta
     4261 aggatcgccc tctggcggcg attcgatctt gatcacgcga gcaccgaggt tggccagcaa
     4321 ggccgaagcg tacgggccgg taacaaaggt agcacactca agaacggtaa cgtgttgcaa
     4381 agcttggatc gacatcaaac ttccttcaga cgtgggcgag gttcgaagat caccggtgcg
     4441 gggactacta gcccgcgcct tccttcattt tactccaaag gagtctacat ttaatcgatt
     4501 gggttttcag cctctttctc atctaaaatg attttgatta gccccagttg ctattagcac
     4561 tagcggaatc ggaattgcca agaaccgtcg tcctggaaca cgccgatctg cttgacgctt
     4621 ccaggacccc aaagttcaat acaaccagat cacccccaga ggagctcgat agcttcgagg
     4681 ctcaaaacaa ggatcatgag tatcgcctcg agcaaaggct aactacccga aacatgacct
     4741 tggcagaatt gcactttgta ttggcaggtc tccagctgcg gatggaatgc gttgcagaga
     4801 gtcggcctcg cttcaacgaa ctatcggcat cttcaaactt tcaccgcgtt ttgtttaaga
     4861 tgtgagtgct gttacaagtt cttcagtaca gataaatgag aaattgatgc ggctattaac
     4921 actattagtc atttcttctc tgttctactt taccggctgt cattctttga ccgtaaatac
     4981 tcagataaac gggattttgc gcgtccatcc atccagtccc cggtacttta ctgacaattc
     5041 cggaaaagcg atttatttgt cgggagaaca aatcttcgtc gatcttcagg ataatacatt
     5101 taacaaatcc tctatttaca attatcaaga agccttggat ttctcctggt acatgcgatt
     5161 catgagggat agaaatctta actacataag gaactggact ctgatgagca caggtggggc
     5221 gtcgggagtt atagcgagcc ccatgcctta taagagagtc tccggatatg gcaacgctct
     5281 cgacggggag cctaagtttg acctgaatca attcaaccaa tcattttttg accgaatgcg
     5341 ggctcgggta atcgaagcct gcagaaacgg aatatatcta agcatcatgt tctatgatgt
     5401 ctacgctttt tcaaattttc cacgcgatcc gatgtgggcc gggaatgtct tcaatcctgt
     5461 aaacaacata aacagcgtca acgcagattc aaatggcgac gggtggggtg tggaattttt
     5521 caccagcccg tctccggaga tcaaggctta ccagcaaaaa tacatccgga aagttatcga
     5581 tacggttaat gatcttgata acgtgttttt tgaaatatcc aacgaggctg cggggcatca
     5641 gtggcacacg gaaattatcg agtatgtgaa aagctacgag tcctccagac caaagcagca
     5701 tttggtgttt tacagtcccg gaggattaag cggagccggc acttatgatt ttacctccta
     5761 tgatgaagtc gtaggctctg cggcggatgt ttttgccggg gcgactggtt ttggcgacca
     5821 ttacagaacc gatcctccgg aaaacaattc cggaaagccc gctatttttg acatggacca
     5881 catagaggcc ggtgaagact tacttaaaaa cgaccacacc tttgcctgga aggcttttac
     5941 tcgggggtac catttcaatc tttacgatcg gccgtttgaa gagccgcagg ctgaaagcgc
     6001 ggcctgggaa attgcccgta agaatgctgg agcgatcgga aacttcgcca ataagaaatt
     6061 tgccgatctt tccaaaatgc atccgcagtc tggcctaagc tcaaccggtt acgcgcttgc
     6121 gaatccgggt tcggagtatt tgatttatca atccggaagc ggaagctttt cggtaaattt
     6181 ggaggccgga acttacaatt acgaatggta caatccgtcg atagcttcac ccgcgggcag
     6241 cggttctatt actgtttccg gcggctatca atcttttacc cctccttttg aaggagacgc
     6301 cgttctttac ctcactggcg gctctgccgg ccgtaagcat aatcttgcca acacgcacgc
     6361 gattcacgac ttacatcaga atctcattac gtaacgaaag tgctgccaaa gacaagtagt
     6421 tcactggcac cgggagttgc gcggaggcaa ccaggagccc agccgtagcg cgagccgttt
     6481 atcaacaaac tcacgtcgtc gggccttagg tgcgccgtta gtggcatcag gtctacgcct
     6541 ggaaacatca atgcccctgc acaccgcagg cgatgccgtt ggattattgt gaatgaaaat
     6601 tcggcggggc gagctaactc gttgaatgta agaaacgtgg cgcgatccgt gcgcgcatgg
     6661 acaagattat atcgtgacac aatcgatagc taaacggatc actgcgcatg cgtttgcggt
     6721 tcagttgggt ttacacgtcc catcccttaa cggagatgtc aacgccgttg ccttcgggat
     6781 cgtggagtcg gacttcagca aacggaatgc gactcgggcg agctgcgcca cggcgagtcc
     6841 cggccttttc ggcgcgatcg cggatctgct caagatcatc tacgtggaac ccgaaatgat
     6901 taaagccatt cttttcctca cgcaccctct ggtacaaaag cgcgagattg aagtatccat
     6961 ccgaaacgaa gatcgatgaa cggtcgctgt gagccacttc tttcatgcca aacgcctcgc
     7021 cgtaaaatgt cgacaaccgt ttggggtcgg cggtgtaaag cgcgatatgc cggattcggt
     7081 agcgggtttt ctccggcgcc acaccgaaat ccttggtcgt gagatcaatc agattatgat
     7141 ccgggtccgc cacctgagtc tcactctgcg gcgaggcctg cccaaccttt tctgattttt
     7201 gctcaacctc ctccagatct tcgacttgaa agccgctggc gtggagtccg gtggcatctt
     7261 tcttaccctc cttgagtaag gcaagagtga acacgccgtc cgaaagacga accaactggg
     7321 gatcttccga gacggttttc agctcgaaaa ccgagcggta aaaatccgcg agccggccgg
     7381 gctcttgcgc tttaatcgtg agacaacgta actgagacat gaaatccctc cttgggccgt
     7441 tgattaggtt cccgggtatg gctgatcatc gcggcgcaac cagtatttca tcacgcggaa
     7501 aatagtccat gatctctgcg ccattttgtg tcaccaccag catgttttcc aagcgcgcgc
     7561 cgccgacgcg ggtctctccc tcgcgtgatt ccaccgcgat ggtcatgccc tcttcgagaa
     7621 gttgcggatg ctcgaatgac cacaaccggt tgatgatcgg agcgtcataa ccagtgctgg
     7681 taccaagccc gatgccatgg ccgatctcgc tggcgagcac ttcgacttcg ctcttatagc
     7741 cccacttgct cgccggcgga aaatgcttcg ccgcatctgc cgtcgtcttg cccggtttga
     7801 tctcctcgat cacggcatcg aggcgctcgc gaacttgttt ataccaatct ttttctttgg
     7861 ccgtcggatt gcggccgacc ataaacgttc ggtaagtaca acttccataa cccatgtaag
     7921 tggcgccgca gtaggagcca tacaccagat cgcctacttg aataattcga ctggttgcat
     7981 gacagccgcg atcgaaagtc atgggcccgg tgcgccagcc tcccggcacc gctgcttcca
     8041 aaccgatctg atagcccgcg ctcgcgccga ttgtcatcaa atccgtatcc cgcattcccg
     8101 gcttcaggct ttcccagatc cgataccaga cgccatcgac catcgcggcg caggtcttca
     8161 agcaactgat ctcatcctga ttttttacca tgcgcgcttc gagcagtagc tgccgtgtgt
     8221 cgacgatatt cttgatcccg gcgttgatca acgcttcgcg cgcattgccg tcgaagctgc
     8281 ctaaccccaa tttttcgccg agcagtccgc gtttctcaag ctcggttttg atttcggcgg
     8341 caaaggcttt ggcttcaacg gcgcaggctt ccggacccgg cgaaccggtg agccaggagc
     8401 gagccgggcg ccactctttg atccaggggc attggtccgg catctgatga tagtaaccat
     8461 cgtgctcgta catcaccgga tcatgctcag caaagaacag cacgtaacgg cacatgggat
     8521 aagcaaaacc cttgagcgcg gtgagatagc gaatattgtg gtgattggct tcgagcagcg
     8581 cggcgatgcc gtattttcgc atcagcattt gcgcgcgttc aacccgttgc cggcgcatac
     8641 ggtccggatt gaaacgctcc tgccaatccg ctgcttcggg accgaacgtc aacttggctg
     8701 catagttggg catggctaac ctccggacta tcgcttccac tcgatgctga acgctggcgc
     8761 ttcaactctt ggcgcgaatc tatcttgaac cattcgccgc ttgcaacagt tcttctcggc
     8821 tacgcctgtg gcaaccttcc caatccttta cgttgaaccg tttaacgttg atctgttgaa
     8881 actattattg tgcgtcaact tcgattgacc gttactactt cctgtagtat gcagccaaac
     8941 atgcgcgcta ctcgggtggg taactgcatg aaacgaacat cggccgtact cacgtgggca
     9001 agtttgcttg tggtcattgc ggctgtccat ccggcctggt gtgccgagag aagccccgaa
     9061 aaagttcgca tcgcagtatc gtcgaagtcg ctgggatttt tggatgtgtg ggcggcgcgc
     9121 gagcgcggct tctatcgcaa acacggcatc gaagcagaaa tcatcgccat gcgtccgccg
     9181 cttaccatcg gcgccatcca ggccggtgag attgattatg ccttcggcgc cagctcgatt
     9241 tcccgtggca gcatgtcggg cgctccggta agaatcgtca gcctttcgct taagacttcg
     9301 tttcacactc tggtggcgcg ctcgaacatc aaatccatcg ccgagatgaa aggtaaaacc
     9361 atcgccgtca ccatcggcgc tgccgatgac ttcgtcgcgc gtcttttggt gcgccggggc
     9421 ggccttgacg tgcgcgatgt cagcttcgtc aacatgggcg gatcggacac ccgtttttcc
     9481 gcccttgcca ccgggaggat cgatgccact ccgctttcat tacctttctt cgtgatcgcc
     9541 aagcggcaag gctacaatct cttgggaagc gccgccgatg tcatcgatat ggcgaccgtg
     9601 ggcatcggca catcgacccg caagatccag caggaacgcg agcaggtaaa gaagatggtg
     9661 cgcgcgcaac tggatactct gcgctggatc aaaacgcaaa agcccgaagt cataccattt
     9721 cttcagaaat actttgatct ggaggaggat gtcgcggccg aatcc
//
LOCUS       DASUTG010000796         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1602694, whole genome shotgun sequence.
ACCESSION   DASUTG010000796 DASUTG010000000
VERSION     DASUTG010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602694"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /gene="glgP"
                     /locus_tag="VF089_18480"
     CDS             complement(<1..1014)
                     /gene="glgP"
                     /locus_tag="VF089_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX6176004.1"
                     /translation="MTTLNHRTTAYFSMEIGLVTEMPTYSGGLGVLAGDTVRAAADLK
                     VPMVAVTLLHRKGYFYQRLDRAGRQIEEPVVWAVNDFLTELPERASVTVEGRAVALRA
                     WIYHVAGPGDFSLPVYFLDSDLPENSEWDRTLTDYLYGGDLHYRLCQEVILGIGGVRM
                     LRALGQTDIARYHMNEGHSSLLALELLDEEARRAGRPSPNRDDVQAVREKCVFTTHTP
                     VVAGHDQFPLALVHQIIGRREVEEMKDVFCCGDLLNMTFLALNLSHYVNGVAKKHGEI
                     TRLLFGGYDIDAITNGVHAASWAAKPFAELYDRYIPGWRQDNYSLRYAMSIPKQEIWQ
                     AHAQ"
     gene            1515..>2121
                     /locus_tag="VF089_18485"
     CDS             1515..>2121
                     /locus_tag="VF089_18485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX6176005.1"
                     /translation="MLLVLLVIFPSMIAVIFYALSEQRREARLQAQETALRMARLSAR
                     EQETLITGAHHLLMTLAELPEVRERKPAKCDELFAGIIKKFPYYTNIGAVSPKGDVFC
                     SGLPNRSSNANIADRGYFQEALNERRLGISHVLVGRMSGKPNITLAYPSFDRDGPVHA
                     VVFVGLDLTWLNNIAATAQLPPQATLLALDQKGTVFVRYSAQ"
BASE COUNT          506 a          602 c          553 g          460 t
ORIGIN      
        1 ctgtgcatgg gcctgccaga tctcttgctt ggggatgctc attgcataac gcaaactgta
       61 attgtcctga cgccagccgg gaatgtagcg atcataaagc tcggcgaacg gcttagccgc
      121 ccagctggcc gcgtgaacgc cattagtgat cgcgtcgata tcgtaaccgc cgaacagcag
      181 ccgagtaatc tcgccgtgct tcttggcaac accgttgacg taatggctca agttgagcgc
      241 caaaaaagtc atattcaaga gatcgccgca gcaaaaaaca tccttcattt cttcgacttc
      301 gcgtcggccg atgatctgat gcacgagtgc caaaggaaac tgatcgtgtc ctgcaacaac
      361 gggcgtatgc gtggtgaaga cgcatttttc gcggacggct tgcacatcgt cgcggttcgg
      421 cgacggcctg ccggcgcgac gcgcttcttc atccaagagc tcaagcgcaa gtaaactcga
      481 atggccctca ttcatatggt agcgcgcaat atcggtttgt cccaaggctc gcagcatgcg
      541 tacccccccg ataccgagta taacctcctg acacagccgg tagtgcagat cgccgccgta
      601 tagataatcg gtcagcgtcc gatcccactc ggaattctcc ggcaagtcac tgtcaagaaa
      661 atagacgggc agcgagaaat ctcccggccc ggcgacatgg tagatccacg cgcgtagagc
      721 caccgcacga ccttcgacag tgaccgaagc ccgctccggt aactcggtaa gaaaatcatt
      781 cacggcccaa accaccggct cttcaatctg gcgcccggcg cggtcgagcc gctggtagaa
      841 gtagcccttg cgatgcagca aagttaccgc gaccatcggc accttgagat cggcggccgc
      901 ccggaccgta tcgccagcga gcacgccgag gccgccgctg taagtcggca tctccgtcac
      961 gagaccgatc tccatggaaa aataagcggt ggttctgtgg ttcaatgttg tcatgatgaa
     1021 ttttccctta cgcctgcgcc cgaactttac cgcaatgccg aacccagtga atactggcga
     1081 gaacttgcgc aggttgaatg caccgaatca aactacctgc caaagcaaac ttctgacatc
     1141 tagcagagca atctcagcaa cgaacctagg tttataatcc aatccttgtg atttggcgat
     1201 gatacctttt ttggatcgca gagtccgtgc catcggctcg aaggcgtcat tacggttccg
     1261 cgaaatttag gctgattaat tcgcacagat gagaaaaaac gggaacggtg ctttgggcga
     1321 atacccgatg ttgcacgtgg cctcaagcct attcgccgat atatgacgca agccgcattt
     1381 ggccctataa ggagaggttt ttgcaatctt tttgcagtaa agattggcca tcgggtgcta
     1441 tcaaaaaatc ggacggacgg agataggtcc acagcgcgat gcacatccac tttaagaaaa
     1501 tccggaactc cctgatgctt ctcgtcctct tggttatttt tccaagcatg atcgcagtca
     1561 tcttctatgc actctcggag cagcggcgtg aggcacgcct ccaagctcaa gagactgcgc
     1621 ttcgaatggc gcgactttcg gccagggaac aggaaacgct gatcactggt gcccaccacc
     1681 tgttgatgac cttggcggag cttccagaag tgcgggaacg gaaaccggcc aagtgcgacg
     1741 agcttttcgc cgggatcatc aagaaatttc cctattacac aaatattggc gcagtgagtc
     1801 ctaaaggcga tgtcttctgc agcggtctac cgaaccgctc tagcaatgca aacatcgccg
     1861 accgaggtta tttccaggaa gcccttaacg agcggcgcct tgggatcagc cacgtactgg
     1921 tagggaggat gtccggcaag ccgaacataa ccttagccta tccctctttt gatcgtgatg
     1981 gacctgttca tgcagtggtt ttcgtcgggt tagatttaac ctggttgaac aacatcgccg
     2041 ccaccgcgca actgccgccc caggcgaccc ttctggcgct cgatcagaaa gggactgtat
     2101 tcgttcggta ttccgcacag a
//
LOCUS       DASUTG010000797         7555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1157997, whole genome shotgun sequence.
ACCESSION   DASUTG010000797 DASUTG010000000
VERSION     DASUTG010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157997"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF089_18490"
     CDS             <1..207
                     /locus_tag="VF089_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176006.1"
                     /translation="PPAVPPERVKILREAYNKSLKDPELLDEVKRSRLDMEPITAEEI
                     ESTYKELMDQPAAVVDLVKKLEAN"
     gene            complement(268..2538)
                     /locus_tag="VF089_18495"
     CDS             complement(268..2538)
                     /locus_tag="VF089_18495"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6176007.1"
                     /translation="MKTVEITRRQFLQTGGALIVSFSLFSPARKALAQFATLPSGDID
                     PTSLDSWLAITQDGFVTFYTSKVELGTGTITALAQIVAEELDVPFDKIKMDSGDTSRT
                     IEQGSTVGSRTIERAGPQIRQAAAAARQELLKLAAAQLNAPVEKLTITEGIVSVAGES
                     SKKISYGQLIGGKKFNTKITATGTGWDMKVAPEVKAKNPKDYKVVGKAIKRTDMPTKV
                     TGEHDFIHNVRIPGMLHGRVVRPPVINTEPIDIDEDSIRGIPGVVMIAREGKFIGVIA
                     KTEWAAIKAAQALKVTWAKPASKVPASADEIYKYLRSSKPVRSQKAVEKGDVAASLSK
                     SKKIYQASYRWPFQMHGMIGPSCSIADVKGDKATIWSGPQGPFRTRGTIARLLNIPEQ
                     NVRVIYHEASGSYGRMSTDDGAEDAALLSRAVGAPVRVQWSRHDEHGWEPKGPAQVDE
                     AKAAVESDGRVLAWDFADYSQPWTASGSTPLLASLQIGITPKNPGGSNGTQSGGEIYA
                     VPNQRILAHLINWHFPEPIPLRTSNLRAPGDVARCFASEGFLDEIAADLQLDPVELRL
                     KNLTGNKRAIECLQAAADKANWQKRRSPAASPSGNIATGRGIAITQRAGTYVAAVAEV
                     EVNKTTGQIAVKRIVCAHDCGLMINPDGVKNQVEGNIIQGVSRALYEEVTYDANGVTS
                     LDWATYPILRFPDVPELDIILINRPEMQPLGAGEGATIPPAAAIANAIFDAVGVRLRE
                     GPFTPKRVLAALQKKA"
     gene            complement(2554..3018)
                     /locus_tag="VF089_18500"
     CDS             complement(2554..3018)
                     /locus_tag="VF089_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008016008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX6176008.1"
                     /translation="MPKISLRVNGKARVVDTESDVPLLYVLRNDLELNGPKFGCGMAQ
                     CGACTVIMDGNAIRSCVTPVGAVQNKPVTTLEGLGTTKKLHKIQQAFIDEQAAQCGYC
                     INGMIMTTKALLDKTPRPTDVQIKQALASNLCRCGTHIRILRAVKRASGQQV"
     gene            complement(3359..4627)
                     /locus_tag="VF089_18505"
     CDS             complement(3359..4627)
                     /locus_tag="VF089_18505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176009.1"
                     /translation="MNDQTTSVAAASVTSDATPTLEATLRQKRMALGLISGCHVLNHL
                     QYSITSVIFPVMMTELGFGLVGLGFISALSSFVGQGLQVIYGFLAAFFKRTTMLAIGN
                     IVVGLTAMLQYFIGSYPQLVAARVAIDAGSSPQHPLGSSILSRYYPKARGWALTFHHS
                     AGSLGSFIGPAAASLALLYMGWRSAFVIFGSLSLLMGLTLFWVSDHSNTRESAGGKTK
                     TKAGFDAYIQCLRNRNILFTSLVLMAGAAGRGTGINMTYLVPFFMAQYGVSASGGGLL
                     LTLMQGAGLVGPLAIAWFSDRSGKRRGITQLTLVLSAVMTIWLVQHQSLSILFYLNLI
                     LYGAFVQARGALTQTMIGDFATPELTDAAFSIYYFVGFISGPIWTLIIGYVMQHYGFA
                     PAFYIAAGTYIAGMLLLTFVKEEPKTGKSS"
     gene            complement(4762..6306)
                     /locus_tag="VF089_18510"
     CDS             complement(4762..6306)
                     /locus_tag="VF089_18510"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX6176010.1"
                     /translation="MDNPQRSSLGAGSKRNLAVTGVMLVIFLFAIDATIVATSMPTIV
                     AQLGGLELYSWVFSIYMLSSALTTPLFGKLSDLYSRRRLMLIGIGIFVVGSLLCGAAQ
                     SMEQLVLFRAVQGLGGGAIYALSFIVVGILFQPEERARMQGIISGIWGVASVLGPLAG
                     GIIVEHWHWRWAFFVNLPITAVAIGLIVVGLKEAGHKRRAHKLDITGMLTLLAALLLL
                     FYALSHSAHSQQPIDTILGGLIFLAVIFLLIFYFIERRAAEPVIPLDLFHSSSYRTST
                     AVATLAAMGVFGAISYLPLYLQGVTGLSASRAGMVLLFLSLAWTLGSLLAGQASNRFG
                     YRAVAASGMLLMAIGYSFFVLFGSALTVAWVIVSATLIGTGMGMANLTTLVAVQNSVS
                     PQRIGVATSTLMLFRTFGGAFSVSLMGTVLLHQMQRGLSKLSTSGLPSTLLDKLAHPQ
                     NLLEPATRMQITPELLPRLIAILSNALWYAFLTGFVLMAIGVIASLVMPGARSAEPAK
                     RHERAG"
     gene            complement(6727..7374)
                     /locus_tag="VF089_18515"
     CDS             complement(6727..7374)
                     /locus_tag="VF089_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6176011.1"
                     /translation="MKILKLSYVLALALSFDGSYAYAQDQQTNPGSKAPKSQGTSSGK
                     QLRSPDVVYVPTPAEAVNAMLKVAKVGKDDVVYDLGSGDGRIPIMAVQKYNAARAVGI
                     DINPDRIKEAQANLKAAGVGDRVRFLNEDLFQADISEATVVTLYLLPSLNLKLLPKLL
                     SELKPGTRIVSHAFDMGSWKPEQTLNAGGSTIYFWTIPAKGTPEYEAASSAASKT"
BASE COUNT         1653 a         2074 c         2215 g         1613 t
ORIGIN      
        1 ccgccggcag tcccgccgga acgggttaag attctgcggg aagcttacaa caaatctctt
       61 aaggatcccg aattactcga cgaggtaaaa aggtcgagac tcgacatgga gcccatcacc
      121 gcggaggaaa tcgaatcgac gtataaggaa ctcatggacc aaccggcggc tgttgtggat
      181 ttggtcaaaa agctcgaggc gaactagccg cgaaaatagg ggacaggcaa cttttcataa
      241 aaacaaaatt cgtgtccctc cacacgatca ggctttcttt tgcagcgctg ccagcacgcg
      301 cttgggagta aacggccctt ctctcaaccg gacgcctacg gcgtcgaaga tcgcgttggc
      361 aatcgcggct gccggtggaa tcgtcgcacc ttcgccggcg cccagaggtt gcatctccgg
      421 acgattgatc aaaatgatat cgagctcggg cacatcggga aagcgcaaga tcggatacgt
      481 cgcccaatcg aggctggtca cgccattggc gtcataggtg acctcttcat acagcgcgcg
      541 gctgactcct tgaatgatgt tgccctcgac ctggttcttg actccatcgg gattgatcat
      601 cagaccgcag tcatgcgcgc agacgatgcg cttgaccgcg atttgacccg tggtcttgtt
      661 tacttccacc tccgcgactg ccgccacgta cgttccggcg cgctgcgtaa tcgcaatgcc
      721 gcgtcccgtt gcgatgttgc cgctgggcga cgccgccggg gaccgccgtt tctgccaatt
      781 cgccttatcg gcggcggctt ggaggcattc gatggcgcgc ttgttaccgg ttagattttt
      841 cagcctcaat tccaccgggt cgagttgcaa atcggcggcg atttcgtcga gaaaaccttc
      901 gctcgcaaaa cagcgcgcca cgtcgccggg cgcgcgcagg ttgctggtgc gcaaggggat
      961 cggttcgggg aaatgccagt ttatcaggtg agcaagaatt cgctggttcg gcacggcgta
     1021 aatttcgccg ccgctttgag tgccgttaga tccgccggga ttcttcggcg tgataccgat
     1081 ttgcagcgaa gccaggagcg gcgttgaacc ggacgcggtc cagggttggc tgtagtcggc
     1141 aaaatcccaa gccaaaaccc tgccgtcact ctccacggca gctttagctt cgtccacttg
     1201 cgccgggcct ttgggctccc aaccgtgttc atcatgcctc gaccactgca cgcgcacagg
     1261 cgcgccgacg gcgcgtgaca ataaagccgc gtcttccgcg ccgtcatcgg tgctcatccg
     1321 gccataggag cctgaggcct catgataaat aacccgcacg ttttgctccg ggatgtttaa
     1381 caacctagcg atcgtgcccc gggttcggaa gggtccttga ggacccgacc agatagttgc
     1441 cttgtcgcct ttgacatcgg cgatcgagca tgacgggccg atcatgccgt gcatttgaaa
     1501 cggccagcga tagctggctt ggtagatttt tttgctttta ctcaagcttg ccgccacgtc
     1561 tcctttttcg actgcctttt ggctgcggac cggtttgctg cttcgcaaat atttgtaaat
     1621 ttcatcggcg ctcgccggca ctttcgacgc cggctttgcc caagtaacct tgagggcttg
     1681 cgcggctttg atcgccgccc attcggtttt ggcaatgaca ccgataaatt ttccttcccg
     1741 cgcgatcatg accacgcccg gaattcccct gatcgagtcc tcgtcgatgt cgatcggctc
     1801 ggtgttgatc accggcgggc gcaccacccg cccatgcagc atgccgggaa tgcgcacgtt
     1861 gtggatgaag tcgtgttcgc cggttacttt ggtcggcata tcggtgcgtt tgatcgcctt
     1921 gccgacgact ttatagtctt tggggttctt cgctttgacc tccggcgcga ctttcatgtc
     1981 ccagccggtg cccgtggcgg taatcttggt attgaatttc ttgccgccga tgagctgacc
     2041 gtaggagatt ttcttcgacg actctccggc aacgctcaca attccttcgg tgatggttaa
     2101 cttctctacc ggcgcgttca actgcgcggc ggcgagtttc aacaattcct gtcgcgccgc
     2161 ggccgcggct tgacggatct gcggcccggc ccgctcaatg gtgcggctgc caacggtcga
     2221 tccctgctcg atcgtgcgcg aggtatcgcc tgagtccatt ttgatcttat cgaacggcac
     2281 gtccagttct tcggcgacaa tctgtgcgag agcggttatc gtgccggtgc cgagttccac
     2341 tttgctggtg tagaaagtga cgaagccgtc ctgggtgatg gcgagccagg aatcgagaga
     2401 agtcgggtcg atgtcgccgg atggaagagt ggcgaattgg gccagggctt tgcgcgccgg
     2461 agagaataaa ctgaagctga cgatgagagc gccgccggtt tggagaaatt gcctgcgagt
     2521 gatctccacg gttttcatgt tcgtcgctcc ttcctatact tgttggccgg acgcccgttt
     2581 taccgctcgt agaatgcgga tatgggtgcc gcagcggcaa agattgctgg cgagcgcttg
     2641 cttgatctgc acgtcggtgg gtctgggagt tttatcgagc aacgccttgg tcgtcatgat
     2701 catgccgtta atgcagtagc cgcattgggc ggcttgctca tcgataaagg cttgctgaat
     2761 cttgtgcagc ttcttggtcg tgcctaaacc ttccaacgtg gtgaccggct tattctgtac
     2821 ggcccccacc ggcgtgacac aggagcgaat cgcgttgccg tccataatca ccgtgcaagc
     2881 gccgcactgc gccatgccac aaccgaactt ggggccgttg agttccaagt cgttgcgcag
     2941 gacatagagc aggggaacat cggattctgt atccaccact cgcgctttgc cgtttactct
     3001 aagtgagatc ttgggcatgg ctggctcctt ctgagtcagg atcggcgtgt actttggctc
     3061 tatttacgag aaggcgctgc gcaaagtcaa tattttgcta agatgatggg accgtctcgg
     3121 tgagagctag aacggcccag ttcggttact gcgacacgat accgcagtgg tgtttcaggt
     3181 tacggcagca ccacgctcgc ggatgaacag gtggattgtt ggaaaggtga ctcatggatc
     3241 aaccaaactg atgaagattg gaagaccttc accacactga gaatcaaatt cacggacagg
     3301 cgttgacagg ctctttccaa gcgtacgtgc gtgttcgtgc taggtgagag gatgggtctc
     3361 agctggactt gccggtcttg ggctcttctt tgacaaacgt cagaagcaac atgccggcga
     3421 tataggttcc ggcggcgata taaaatgccg gggcgaaacc gtaatgctgc atgacgtagc
     3481 cgatgatcag agtccagatc gggccggaga taaagccgac gaaatagtaa atcgagaacg
     3541 ccgcgtcggt gagctccgga gtggcgaagt cgccgatcat cgtttgcgtc agcgcccccc
     3601 tagcttgaac gaaggcgccg taaagaatca ggttgagata aaacaaaatg ctcaaggatt
     3661 ggtgttggac gagccaaatt gtcatgacgg ccgataacac cagggtcagt tgggtaatgc
     3721 cgcgccgttt gccgctgcga tcggaaaacc aggcgattgc caggggaccc accaatcccg
     3781 ctccttgcat caaggtcaag agcagcccgc cgccggacgc gctgacaccg tattgggcca
     3841 tgaaaaaagg caccaggtaa gtcatgttga taccggtgcc gcgtcccgcc gcgccggcca
     3901 tgagcaccaa tgatgtgaag agaatattgc ggttcctaag gcactggatg taggcgtcga
     3961 aacctgcttt ggtttttgtc ttgccgcctg cgctctccct cgtgttgcta tgatcgctga
     4021 cccagaaaag cgtcagtccc atgagcaagc tcagcgatcc gaaaatcacg aaagcgctgc
     4081 gccagcccat gtagagaagc gcgagtgagg ccgccgccgg tccgatgaac gatccgagac
     4141 tgcccgccga gtgatgaaac gtgagcgccc acccccgcgc tttcggatag taacgcgaca
     4201 gaatgctcga cccgagcgga tgctgcggac tggagccggc atcgatggcg acgcgcgcgg
     4261 cgaccagctg aggatagctg ccgatgaagt actgcaacat cgcggttagt ccgacgacga
     4321 tgttaccgat cgccagcatg gtggtgcgtt taaaaaaagc ggcgagaaaa ccgtagatga
     4381 cttgcagccc ttggccgacg aagctcgata gcgccgagat aaagcctagt ccgactagac
     4441 cgaagccgag ttcggtcatc atcaccggga agatcacgga agtgatgctg tattgaaggt
     4501 gatttagaac atggcaaccg ctgatcaggc cgagcgccat gcgcttttgc cgcagtgtag
     4561 cttcaagagt gggtgtcgcg tccgacgtga cgcttgcggc agcgacggag gtggtttgat
     4621 cgttcatgaa agtaagtctc gagtgcttgg cagcatggag tgttgagatc agatgtacaa
     4681 gttgatgagg ttcgcgtcaa acgcggcggc gttatcgaga caatccggat gttacccagc
     4741 gggtttcaca atccactggt tctacccagc gcgctcgtga cgctttgccg gttcagcgga
     4801 cctggcgccc ggcatcacca gactggcgat cacgccgatt gccatgagca caaatccggt
     4861 gagaaaagca taccaaaggg cattgctgag aattgcgata agtctcggca gcagttccgg
     4921 cgtgatctgc atgcgcgttg ccggctccag aaggttttgc ggatgggcga gcttatcgag
     4981 cagtgtgctc ggcaaaccgc tagtggagag tttacttaag ccccgctgca tctgatgcag
     5041 cagcacagtt cccatcaagc tcaccgaaaa tgcgccgccg aaagttctaa atagcattaa
     5101 cgtcgaagtt gccacgccga tgcgctgagg tgaaacggaa ttctgcacgg ccactaaggt
     5161 tgtcaggttg gccatgccca tgcctgtgcc aatcaatgtc gcgcttacga taacccacgc
     5221 gacggtcagt gccgatccga aaagaacaaa gaaactgtag ccgatagcca tgagcaacat
     5281 gccgctggcg gcaactgcgc gatagccgaa gcgattgctc gcttggccgg cgagcaagct
     5341 gcccaaggtc cacgccaggc tcagaaacaa caacaccatc ccggcgcggg aagcgctgag
     5401 gccggtcacg ccttgaaggt agaggggcag ataactgatt gcgccgaaca cccccatcgc
     5461 tgcaagcgtt gccacagcgg tcgaggttct ataagagctc gaatgaaaca agtccagcgg
     5521 gatgacgggc tcggctgctc ggcgttctat gaagtagaag atcaggagaa agatcaccgc
     5581 cagaaaaata aggccgccaa ggatcgtgtc gatcggctgt tgtgaatgag cgctgtggga
     5641 cagcgcgtaa aacagcagca acaacgccgc cagtagggtg agcatgccgg tgatgtcgag
     5701 tttatgcgcg cgccgtttgt ggcccgcctc tttaaggcca acgacgatca agccgatggc
     5761 gacggcggta atcggtaaat tgacgaagaa tgcccaacgc caatgccagt gttcgacgat
     5821 gataccgccg gccagcggcc ccagcaccga tgcgacgccc cagatgccgc tgatgatgcc
     5881 ctgcattcgg gcgcgctcct ccggctgaaa caagatgccg acgacgatga acgaaagcgc
     5941 atagatcgcg ccgccgccca ggccctgcac ggcgcggaat aacacgagct gctccatgct
     6001 ttgcgccgcg ccacacaaga gcgagccgac gacgaagatg ccgataccga taagcatgag
     6061 cctgcgccgg ctgtagagat ccgatagctt gccgaacagc ggcgtagtga gcgcagaact
     6121 gagcatgtaa atcgaaaaca cccagctata gagttccaga ccaccgagct gggcgacaat
     6181 ggtcggcatc gaagtggcga cgatagtggc gtcgatggca aataagaaaa tcaccaacat
     6241 gacgccggtc accgccagat tgcgctttga tccggcgcct agagaagatc tttgcgggtt
     6301 atccacgtgg ttgggtcggg cgggatttga atcaagttcc tgctaacctc atggttgaac
     6361 tagtcaatga ggatagtcaa gcaaccgttg ctggggcgac ggctgcgctt tgcaacgttg
     6421 ccgacacacc gggctgagca aatcacgccg gaactcactg cagccgtcga caaaaggcgt
     6481 ggggccacgc ttccgaactc atagccactc gtttggcaaa aactacgggt tcaggatgac
     6541 acgcacggcg atgcggcaga gatcgttaag cgacggttga gaagggctgc cggcaggtta
     6601 atatggaatt cgctcaagga acaaagccgt cacgcagcag aatgttctgc gcgtattgag
     6661 aaagtatcgc taggccggaa gacaggtctt tcaaaccatg tcttccagcc gtagcgcagc
     6721 ggtggatcag gtcttacttg ccgcggaaga ggccgcttcg tactcgggcg ttcccttggc
     6781 gggaatcgtc cagaaataga tcgtactccc accggcgttc agtgtctgct cgggcttcca
     6841 actacccatg tcgaaagcat gcgagacaat gcgggttccc ggctttagct cagacaaaag
     6901 ttttggcagc aatttgagat tgaggctggg cagtaaatag agggtgacaa cggtggcctc
     6961 gctgatatcc gcctgaaaca agtcttcatt gaggaaacgc acgcgatcgc caacgccggc
     7021 ggccttgagg ttcgcctgag cttccttgat gcggtcggga ttgatgtcga tgccgacagc
     7081 tcgcgccgca ttgtacttct gcaccgccat aatcgggatg cgcccgtcgc cggaacccag
     7141 atcatagacc acatcatcct tgccgacttt cgctaccttg agcatcgcgt tcacggcttc
     7201 agcgggtgtt ggaacgtaaa cgacatcggg tgagcgaagt tgcttgcctg agctcgtgcc
     7261 ttggctcttc ggcgccttgg agccgggatt agtctgctgg tcctgggcgt atgcgtaaga
     7321 cccatcaaat gaaagcgcca atgcgagcac gtacgatagt ttaagaattt tcaccgattc
     7381 ctccttgttc atgtatcacg ttgttgagtc tgagatctcc gctgttcctc agagctcatg
     7441 aaaatataac cttaaaacaa gagtaccgat agattgtgca gcttagattg tgcagctttt
     7501 tttcacctcc tggaaaaatt ttgaaaactg agcgatgggt ctgctggtcc gagaa
//
LOCUS       DASUTG010000798         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_418667, whole genome shotgun sequence.
ACCESSION   DASUTG010000798 DASUTG010000000
VERSION     DASUTG010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418667"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2274
                     /locus_tag="VF089_18520"
     CDS             <1..2274
                     /locus_tag="VF089_18520"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX6176012.1"
                     /translation="VGLDPKKNGKLDYVVRSANLTPWLLLANQQGSGSMELTGRASGN
                     IADLQSQGTMKLAGLRLQDTTIKSGSIDFDLRLAGTQSLPQGTMNVRLAEIQAGIPLQ
                     RLDGSIGLSIKKNYEIGIDFKAVDQAKRTHILAGQVDYQPQLIVARLDRLGLTLDDGV
                     WQLSRPATITHRDDSFDIGNLVLRNRAQQLAINGRFATRGKQDVAVTVEGFPISAVSA
                     FLPKPLPMTGTLAVRAQVTGTAAAPEITASVNLTDSSIGGQSYGGVIGDLTYRSRTAD
                     VKLTVRQDADHLLTANGTIPLVLKWDPQWQAEAGGEMNVRLHSAGLSIAFLNAFSGEA
                     AQNITGVFDLDVLARGPLTQPTLRGSFGLRDGAVAVKPLGVQLANITADGGLDGQTIS
                     IRQISARSKEGRLSGSGRLALREYSPEDFKLSLSLSQWPLIETQQYRAMVAGNLEAQG
                     PLAKPLLSGQLEVLNAELRPDLAFLGSGKRPLTRDETIVIIGSRNQSQTSETRQLRAE
                     KQAPDNELLKNARLDLTVLMPNQVWIRHPDANVELRGKLRAVKQTGKDIALTGQIAAV
                     RGWIGFQGRRFNLTRGTIEFTGGETINPGLDILAQYRLQQYQVDVTITGTLAKPALTM
                     RSEPPLEQADILALLIFGKPVNALSRTEQNTLQQSAAEMAGGLAAAKVASAVSDALGL
                     DRLGLDLGEIDITGGQIGFGRYIGNRTYVSISQELAGEAGREVSIEYQIGPDWKITSS
                     TSTTGTSGVGILWHKRY"
     gene            complement(2420..>3409)
                     /locus_tag="VF089_18525"
     CDS             complement(2420..>3409)
                     /locus_tag="VF089_18525"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6176013.1"
                     /translation="RDFILPILLAWILSSLLAPVVRSLKRIRIPEPLSALLIISALLG
                     TLSLGIYRMSEPVASWIQRAPQVLTDVRAKLQNFIRPMAEVQETTKEIEKMAGLGNDQ
                     QAAAVEIKKPGLSQIVFSGAKDFVLAAGVMLILLYFLLASGDLFLLKLVKVLPTLENK
                     KVAVEIYRRIEKDVSTYLSVVTLIYIGFGCVIGVAMYLLGMPNPWLWGVMAAVLSYIP
                     YLGALVGLTTVTLVAILTFEDVFRIVLVPLVYFTVDTIQANLIFPMALGRRLALNPVM
                     IFIWLIFCGWIWGIIGALLAVPLLAIIKIICDQVEQLAPVGEFLGRESKIEPS"
BASE COUNT          848 a          950 c          936 g          675 t
ORIGIN      
        1 gttggtctcg atccaaagaa aaacggcaag ctggattatg ttgttcgctc cgcgaaccta
       61 acgccgtggc tccttttggc gaatcagcaa ggttcaggtt ccatggagct gaccggccgc
      121 gccagcggca atattgccga tctccagtca caaggcacaa tgaagcttgc gggattacgg
      181 ctgcaggata cgacaatcaa gagcggcagc atcgatttcg acctccgcct cgccggtacg
      241 cagtccctgc cgcaaggaac catgaacgtt cgactcgctg agatccaagc aggaatccca
      301 ctgcaaagac tcgacggctc catcggactc tccattaaaa agaattatga gatcgggatt
      361 gacttcaaag ccgtggatca ggccaagcgc acgcatattc tcgcggggca agtggactat
      421 cagccgcaac tgatcgtcgc tcgactggat aggctcggct tgaccctcga tgatggagtt
      481 tggcagctct cccgaccggc aacgatcacc catcgcgacg atagcttcga cattgggaac
      541 ttggtcttga gaaatcgggc ccaacagcta gcaataaacg gccgattcgc cacccgcggc
      601 aaacaggatg tcgctgttac ggtggaaggc ttcccgatca gcgcagtctc ggctttttta
      661 ccgaaaccgc ttcccatgac tggaactttg gcagtacgtg ctcaggtcac cggaactgcg
      721 gcggcgccgg aaatcaccgc atcagtgaat ttaaccgata gtagcatcgg cggacaaagc
      781 tacggcggcg ttataggtga tctcacatac agatctcgca ccgcagatgt gaagttgacc
      841 gtgcgccagg acgccgatca tctcttgacc gccaacggca cgattccgct cgttttgaaa
      901 tgggatccgc agtggcaagc cgaggccggc ggcgagatga acgtacggct ccattccgcc
      961 ggtttgagca tcgcatttct aaatgctttc agcggagagg cggcgcaaaa cataactggg
     1021 gttttcgacc tcgatgttct tgctcgagga ccgctgacac agccgaccct tcgcgggtct
     1081 tttggactac gagatggggc agtggcagtc aagcccctgg gagtgcaact ggcgaatatc
     1141 acggcagacg gtggactcga tgggcagacg atatcgattc gccagatttc cgcccgctcc
     1201 aaagagggcc ggctaagcgg cagcggcagg ctggctttgc gagaatacag tccggaggac
     1261 ttcaagcttt ctctctctct ctctcagtgg ccactcatcg agacccagca ataccgcgcg
     1321 atggtggcgg gaaatctgga agcccaggga cctctcgcca aaccgttatt gagcggtcag
     1381 ctcgaggtcc tcaatgccga gctgcgcccc gacttggctt ttctgggtag tggcaagaga
     1441 ccgttaacgc gggatgaaac catcgtcatt atcgggagtc gaaatcagag ccaaacctca
     1501 gagactcgac agctccgagc tgaaaaacaa gcgccggaca atgagctgtt gaaaaacgcc
     1561 agactggatt taaccgtctt gatgccgaac caggtttgga tcaggcatcc tgatgccaat
     1621 gtggagctcc gcggcaaatt gcgagcggtc aaacagaccg gaaaagacat tgcgctgacc
     1681 ggacaaatcg cagccgtgcg aggctggatt ggctttcaag gtaggcgctt caatctaacc
     1741 cgtggcacga tcgaattcac aggtggcgaa acgatcaatc cggggctcga catcctggcg
     1801 caataccgcc tacagcaata tcaagtcgac gtaaccataa ccggaactct agctaagcct
     1861 gctctgacaa tgcgcagcga accacccttg gagcaggccg atattttggc gctccttatc
     1921 ttcggcaagc cggtcaatgc gctcagtcga actgaacaga acacattgca gcaaagcgcg
     1981 gccgagatgg ccgggggttt agcggcagcg aaggttgcaa gcgcggtctc cgacgcgctg
     2041 ggattggata gactcgggct cgaccttggc gaaatcgata ttaccggcgg tcagatcggc
     2101 ttcggccgtt acataggtaa ccgaacctat gtctccatct ctcaggaatt ggcgggagaa
     2161 gcgggacggg aagtctcgat agaataccag atcggacccg attggaagat caccagttcg
     2221 acgtccacca ctggtacaag cggtgtcggc attctctggc acaaacgtta ctgaccgatt
     2281 catcttcatt tggtcgcgcc gcggggttaa accgcgtcgt aactcgcgac cgagaacttg
     2341 cccaggtcga cgcggcagcg tctctcgctg aaataccggc tgttcacctt ttccatgaca
     2401 gcgccgctca tagaaaacct taggacggct cgatcttgct ttccctgccg agaaattcac
     2461 cgacgggcgc aagttgctcg acctgatcgc agatgatctt aatgatcgcc aaaagcggaa
     2521 cggccaataa ggctccgatg attccccaga tccagccgca gaagatgagc caaatgaaaa
     2581 tcatcacggg gttcaacgcc agccttcggc ccagagccat cgggaaaatc agattggcct
     2641 gaatcgtgtc aacggtaaaa tagacaagcg gaaccagaac aatgcgaaag acatcttcaa
     2701 atgtcagaat ggccacgagc gtgacggtag tgagtcccac cagcgcgccc agataaggga
     2761 tgtacgagag aacggccgcc atgactcccc acaaccaggg attgggcatg ccgagtaggt
     2821 acatggcaac gccgatgaca catccaaatc cgatatagat aagagtcacc accgacagat
     2881 aggtcgatac gtccttttcg atgcgccgat agatttccac tgcaactttt ttattctcca
     2941 gcgtcggcaa aactttcact agcttcaaca aaaacaaatc ccctgaagcg agcaggaagt
     3001 aaagcaggat cagcatcaca ccggcggcta ggacgaagtc tttggcgccg ctaaagacaa
     3061 tctgactcaa tcccggtttt ttaatctcca cggcggcggc ttgctgatcg ttgcccagac
     3121 ccgccatttt ctcgatttcc ttggtcgtct cctgcacctc tgccatcggg cggatgaagt
     3181 tctgcagctt ggcgcggaca tcggtgagaa cctgcggtgc tctttgaatc caggacgcaa
     3241 ccggctccga catacgatag atgcccaaag atagtgtgcc taagagcgcg gagataatta
     3301 gcaaggcgct caaaggctcg gggatgcgaa tgcgtttgag agatcgcacg accggcgcca
     3361 agagagagct caaaatccat gccagaagta tcggtagaat gaagtcacg
//
LOCUS       DASUTG010000799         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_566667, whole genome shotgun sequence.
ACCESSION   DASUTG010000799 DASUTG010000000
VERSION     DASUTG010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566667"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..580
                     /locus_tag="VF089_18530"
     CDS             293..580
                     /locus_tag="VF089_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176014.1"
                     /translation="MSETDKRLFVGIKISTKLQHGLDSPAPGTDRYFKENNGDFLQII
                     TQGEEKLIGRFIKDGFPVGDLDNVSRNVRSIVRIIAGDHRLEEDAVHIYIR"
     gene            771..1238
                     /locus_tag="VF089_18535"
     CDS             771..1238
                     /locus_tag="VF089_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176015.1"
                     /translation="MSKAIEQIHMKLENGTVIRHKVVGYEGRIDGVTEIKACFTKAGH
                     VLDKLNSKETFQYRVMVEGEALRRIAPAEDFEILEGVVDVICPSCKHSFHSKPGIGNK
                     PGGRCQCGGWICPTCLSCQEPKPETPGTRSQSCLKQRDRKARKLAAEKKSKAG"
     gene            complement(1312..2067)
                     /locus_tag="VF089_18540"
     CDS             complement(1312..2067)
                     /locus_tag="VF089_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6176016.1"
                     /translation="MSRTTSWNPATYARDARFVADLGQPLLELLAPKNGEIIMDLGCG
                     DGALTEKIAQTVAVVGADASFAQARAAKERGLSIVVVDGHNLCFKRQFDAVFTNAALH
                     WMQQPENVVEGVWRCLKTDGRFVGEFGGQGNVGTVRSALHQALRRRGIEPSALDPWYF
                     PSAEDFSNLLIKSSFTVRSIEIIERPTQLPGELLDWLEIFAQPFTNAIDKDHRGSFLD
                     EVCQRSVTALRNEDGRWTLDYVRLRFAAIKAEK"
     gene            2139..3335
                     /locus_tag="VF089_18545"
     CDS             2139..3335
                     /locus_tag="VF089_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX6176017.1"
                     /translation="MATKDIDLASLRIDRSFQRGNLPRNTRRKRKILFALIIAVLLTT
                     AIMFLRELFNPAIEVQLATAALTSPSQTKSILTASGYVVARRKAAVASKGTGRLVLLN
                     VEEGDQVKKGQLIARLEDDDVVAARESARQSLLVAEADLYDAKTSYERMRILLQQGLI
                     AQAEYDVAEARFKRVVASIDVARFALKEAEVAVENTRIIAPFDGTVLKKNADVGEIVA
                     PLAGAASSKAAVVTIADMSSLEVDADVSEANITRVAPEQQCEITLDAYPQQRYPGYVT
                     KIVPTADRSKATVLVKIRFKEYDERVLPEMSAKISFLAIGYKADSADHKLLLTVPEAA
                     VATRTGRAVIYQIRDDRAVEVPVSTGQKLGDLIEIKSGIKEGDKIIAAVDDRIEAGAE
                     VAVKGQ"
     gene            3352..4026
                     /locus_tag="VF089_18550"
     CDS             3352..4026
                     /locus_tag="VF089_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176018.1"
                     /translation="MESPLIEVVDLFKSYQRDSLEIPVLRNISLNIAEGDFLAFMGPS
                     GSGKTTLLNLIAGIDKPTSGKILIADTDITELSETELAVWRSHNIGFIFQFYNLIPVL
                     TALENVELPLILTPLSRKERRSHAETALEVVGLSERMHHYPRLLSGGQEQRVAIARAI
                     ATDPAILVADEPTGDLDKVSAEEVLELMHRLNRELNKTILMVTHDPRAAERARSVRNL
                     DKGELA"
     gene            4035..>4424
                     /locus_tag="VF089_18555"
     CDS             4035..>4424
                     /locus_tag="VF089_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176019.1"
                     /translation="MELLKLLLRNTLRHKLRSFLTLTGIGVAVMAFALLRTVVTAWHA
                     GVEASAANRLITRHAVSFVFPLPLAYRDRIAQVRGVNKVTYASWFSGVYIDKNQFFAR
                     LAVDSETFFNVYPEFVIEREQFEAFKRE"
BASE COUNT         1034 a         1139 c         1254 g          997 t
ORIGIN      
        1 tcactaatta tgactccggg catatcagca tttagcagct tttttcaagc tcagctgcgg
       61 tgccagctac taagatagta tttcggtctt tgagagttgg cagggcaagg cagtcggttg
      121 gatcgttgcg acgcgtttac aaggagcaaa gattgtatta gcatacgtac tcgctaaaag
      181 cgttgaagct tcatatagaa cgcacttagc cgccagattg gctcgggtcg cgctttcagt
      241 atcgccgcaa gatgtcatcg cgtgatgact acaagctaga ggggaatgta atatgtcgga
      301 aacggacaag cgactttttg tcggaataaa aatttccacg aagttacagc atggcttaga
      361 tagcccggcg ccgggaaccg atcgctattt taaggagaac aatggcgatt ttttgcaaat
      421 tatcactcaa ggcgaggaga aactgatcgg ccgtttcata aaggatgggt ttcctgtcgg
      481 agacctggac aacgtcagcc gcaatgtgcg cagtatcgtg cgcatcatcg ccggcgatca
      541 ccggctcgag gaagacgcgg ttcatattta tatccgatga gttggcatgg cgctatcgga
      601 tttaagtgta gctaccttcc ttcgtcgcac ttacctgagg agttgtttac cggaagacga
      661 aagtgttaga attagtcctc gtttgggcag cgtgaaatct ttacctatta gcaacgcctc
      721 tgcgctttta aacgagccag aggctttttt ctttctgcaa ggagggcatt atgtcgaagg
      781 cgattgagca aattcacatg aagctcgaaa atgggacagt gatccggcac aaagtggtcg
      841 ggtatgaagg gcggatcgac ggtgtgaccg agatcaaagc ctgctttaca aaggcaggcc
      901 atgttctcga caagctcaat tccaaggaaa cgttccagta tcgcgtgatg gtggaaggcg
      961 aggcactgcg tcgcattgcc ccggctgaag attttgagat ccttgaagga gttgtcgatg
     1021 tcatctgccc gagttgcaag cactcgtttc acagcaagcc gggtatcggc aataagcctg
     1081 gaggccgttg tcaatgcggc ggatggattt gtcccacctg tttatcatgc caggagccaa
     1141 agccggagac ccccggaacc cggtcacaat catgtttaaa gcagcgggat cgtaaagcga
     1201 ggaaactcgc ggccgaaaag aagagcaagg ctggttgagc tttaagctac tgactaatag
     1261 ctgacgtttg tcaactacgg cgatatgagg gggcaacaag ggttgccctt ttcatttttc
     1321 cgcttttatt gctgcaaatc tcaggcgaac gtagtccagc gtccagcggc catcttcgtt
     1381 gcgcaaggcg gttacgcttc gctggcatac ttcatcaaga aaggaccccc ggtgatcctt
     1441 gtcaattgcg ttggtgaaag gctgggcgaa aatctccagc caatcgagaa gctctccggg
     1501 aagttgcgtc ggtcgctcaa tgatttcgat ggaacgaacc gtgaagctcg acttaatcaa
     1561 aaggttcgaa aaatcttccg ccgagggaaa gtaccaggga tctaacgctg aaggctcgat
     1621 gccgcgccgc cgcaacgctt ggtgcagagc cgaacgaaca gttccaacgt tgccttggcc
     1681 gccaaactct ccaacgaaac gcccgtcggt ttttaaacac cgccaaactc cttcgacaac
     1741 gttttcgggc tgctgcatcc aatgaagcgc ggcattggtg aaaacggcgt caaattgccg
     1801 cttgaagcag agattgtggc catcgacaac cacgatggat agccctcgct ctttggccgc
     1861 gcgcgcttga gcaaagctgg catccgcgcc cacgacagcg actgtctgag cgattttttc
     1921 ggtcaaagcg ccgtcgccgc aacccagatc catgatgatc tcaccgtttt tgggcgcaag
     1981 cagttctaga agcggctgac cgagatcggc gacgaatcgc gcatccctgg cataagtggc
     2041 ggggttccag ctggtggtgc gactcatcgc ccatgttctt aacatcggat gatcgggttg
     2101 aaaacggcga ttcgatcggc ttactgtaaa tgtttaaaat ggctacgaaa gacattgatt
     2161 tagcatcgtt gagaattgac cgctcgtttc aacgtggcaa tttgccgcgg aatacgcgaa
     2221 gaaaacggaa aattctgttt gctttgatta tcgctgttct tctgacgacg gcgatcatgt
     2281 tccttaggga gctgttcaat cccgcgatcg aagtccagct ggcgacggcc gcactaacct
     2341 caccgtcaca gaccaaatcg atattgaccg cgagcggtta cgtggtggcg cgccgcaagg
     2401 cagcagtcgc ctccaagggt accggacgtt tagtgctcct gaacgtagaa gagggtgatc
     2461 aagtcaaaaa gggccagctc atcgcccggc tggaggatga cgacgtcgtt gccgcgcgcg
     2521 aaagcgctcg acaaagtctg ctcgttgccg aagccgatct gtacgatgca aaaacctcat
     2581 acgagcgtat gcgtatactc ctccagcagg gcttgatagc gcaggccgag tacgacgtcg
     2641 ctgaggcgcg tttcaaacga gtcgtcgcat ctatcgatgt tgcgagattt gcgctgaagg
     2701 aagccgaagt cgctgtcgag aatactcgga tcattgcgcc gttcgatggc acggttctga
     2761 agaagaatgc cgatgtcggc gagatcgtcg cgccgctggc gggcgccgcc agttccaagg
     2821 cggcggtggt gacgatcgcc gacatgtcgt ctctggaagt ggacgcggac gtgtccgaag
     2881 ccaacattac ccgggtcgcg ccggaacagc agtgtgaaat tactctcgac gcctacccgc
     2941 agcagcgata cccgggatac gtcacgaaga ttgttcctac cgccgatcga agcaaggcca
     3001 ccgtcctggt gaagataagg tttaaagaat atgacgagcg ggttctgccg gagatgagcg
     3061 ccaaaatcag ctttctcgct atcggttaca aagcggacag cgcggatcac aagctgctgc
     3121 tcaccgtccc tgaagcggca gtggccactc gcaccggacg cgcggtcatc taccaaatca
     3181 gagacgatcg cgccgttgaa gtgccggtga gcacaggcca aaagctcggt gatttgattg
     3241 aaataaagag cggcatcaaa gagggagaca agatcatcgc cgcggttgat gatcggatcg
     3301 aagcgggcgc ggaagttgcc gtgaagggac aatagttttt gcactcgcac catggaatct
     3361 ccgctcatcg aagttgtcga tctgttcaag tcctaccagc gtgacagcct ggaaattcct
     3421 gttctgcgca atatttctct taacattgca gagggagact tcctcgcctt catggggccg
     3481 tctggctcgg gcaaaaccac tctgttgaat ctgatcgccg gcatcgataa gccgacctcg
     3541 ggcaaaatcc tgatcgccga taccgatatt accgaattga gcgaaaccga attggctgtg
     3601 tggcgctcgc acaatatcgg gttcatcttt cagttctaca acctcatacc cgtgctcact
     3661 gcgttggaga atgtcgagct gccgctgatt ctgaccccgc tttctcggaa ggaacggcgc
     3721 agtcatgcgg aaactgcatt agaggttgtg ggtctcagcg agcgtatgca tcactatcct
     3781 cgcctacttt ccggtggcca ggaacagcgc gtcgccatag ctcgagcgat cgcgacggac
     3841 ccggcgatcc tggttgccga tgagccgacc ggcgatctgg acaaggtctc cgccgaagag
     3901 gtgctggagt tgatgcatcg attgaaccgc gagcttaaca agacgattct tatggtcacc
     3961 catgacccgc gggctgccga gcgcgcccgg tccgtgcgaa atctcgataa gggcgagttg
     4021 gcgtaggagc agttgtggaa ttgctcaagc ttctcctgcg caacacgctg cgtcacaagc
     4081 tccgcagttt tttgacgttg acgggaatcg gcgtcgcggt tatggcgttt gcgctgctgc
     4141 gaaccgtggt gacggcctgg cacgcgggag tcgaagcgtc ggcggcaaat cggttgatca
     4201 cccggcacgc cgtgtcgttt gtttttcctt tgccgctggc gtatcgagac cgcatcgctc
     4261 aggtgcgcgg cgtcaacaaa gtcacttacg cgagttggtt ctccggcgtc tacatcgaca
     4321 aaaaccagtt ttttgctcgc ctggcagtgg actccgaaac ctttttcaat gtttatccgg
     4381 agttcgtcat tgaacgtgaa caattcgaag ctttcaagag agag
//
LOCUS       DASUTG010000800         4538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1903387, whole genome shotgun sequence.
ACCESSION   DASUTG010000800 DASUTG010000000
VERSION     DASUTG010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903387"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF089_18560"
     CDS             complement(<1..276)
                     /locus_tag="VF089_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX6176020.1"
                     /translation="MPLSDIAIRSAKPRATPFKLFDGGGLYLIVTPTGRKWWRWKYRF
                     GGKEKGLSFGVYPEVSLKAARQRRDDARSQIANGIDPGEARRAVKIAQ"
     gene            complement(443..793)
                     /gene="ssrA"
                     /locus_tag="VF089_18565"
     tmRNA           complement(443..793)
                     /gene="ssrA"
                     /locus_tag="VF089_18565"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(868..1320)
                     /gene="smpB"
                     /locus_tag="VF089_18570"
     CDS             complement(868..1320)
                     /gene="smpB"
                     /locus_tag="VF089_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX6176021.1"
                     /translation="MTGEHNVCVNRQARHNYFIEETYEAGLMLLGSEVKSLRDGKANL
                     TDSYARIQKGEAFLVNAHVSHYPGANQFNHEPTRTRKLLLHAREIERLTGKTTERGLT
                     LVPLRLYFKNGKAKVELGLARGKKLYDKRETLRRKVAEREVERSLKAR"
     gene            complement(1328..1981)
                     /locus_tag="VF089_18575"
     CDS             complement(1328..1981)
                     /locus_tag="VF089_18575"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX6176022.1"
                     /translation="MGEDVPAKNRVRLCENFQRYFLAGLLVFLPIVITLWFLGWVIGL
                     LDGVLDVLPAGVHPNTYLPFAIPGLGALVTVTVILFLGILTRGVATRRFLAAWENVFV
                     KIPIFRGVYTAVQKLVQTFLGQSQNSRQVVMIEYPRKGIYTVGFAMGRAWRELERKQD
                     AQLINVFIPTTPNPTSGFYLLVPTTEISPLDMTMEEALKLITSGGLITPDDRSRRRD"
     gene            complement(1983..2840)
                     /gene="panC"
                     /locus_tag="VF089_18580"
     CDS             complement(1983..2840)
                     /gene="panC"
                     /locus_tag="VF089_18580"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX6176023.1"
                     /translation="MRIIRNTTEMTDWSQAERQQGHRIAFVPTMGALHEGHLSLVREA
                     KRRGDRVVVSIFVNPTQFAPAEDFAAYPRDFARDCSLLEIEEIDVLFHPSAEEIYPLG
                     NQTHVEVQQLSKILCGLQRPGHFRGVATVVTKLFNVVRPDVAIFGEKDYQQLQVIRRL
                     VRDLLLDIEIVGHPIVRESDGVAMSSRNAYLNADQRKAARCLSRSLSKAECLVRQGEV
                     SAKTIVEVVIAELQAEPLAVVEYVAICDAETLEPLDQIRHSALLALSVTIGKARLIDD
                     AVLLRSPGE"
     gene            complement(2890..3693)
                     /gene="panB"
                     /locus_tag="VF089_18585"
     CDS             complement(2890..3693)
                     /gene="panB"
                     /locus_tag="VF089_18585"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6176024.1"
                     /translation="MAEKVTVPGIVKMKHQGKKITCLTAYDYSLARILDDAGVDVILV
                     GDSVGSVVQGQPNTLAVTMDEMIYHTRIVARGCKRALVVGDMPFLSFQVGREQALRNA
                     GRFLQEAGAEAIKLEGGIAMEDTIAAIVNAGIPVMGHVGLTPQSVHRFGGYKVQGREK
                     GQYDIIVRDALAVQEAGAFAIVVEGVPTALAKEITERVTIPTIGIGAGIHCDGQVLVL
                     HDMLGLFDDFTPKFVKRYADIKGSMTAAVKNFVDEVRERKFPGEEHSFK"
     gene            complement(3809..4450)
                     /locus_tag="VF089_18590"
     CDS             complement(3809..4450)
                     /locus_tag="VF089_18590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX6176025.1"
                     /translation="MSNTKYIVVEGPIGVGKSSLAKMLANQFQARSLFERIEDNPFLP
                     KFYQARQTYAFQNQTFFLLNRYQQQIELSQQDLFNQNVIADYLFAKDRIFATLTLSAE
                     ELNLYQQIYSLLNARVPKPDLVVYLQARPEVLYKRVKKRDKKYERGVTFEYLNEVAQA
                     YNQFFFHYDETPLLVVNTSKIDFVESSKDLADLIKEINNMGSGTQHFIPLGSR"
BASE COUNT         1092 a         1248 c         1102 g         1096 t
ORIGIN      
        1 ttgggcaatt tttaccgccc tccgggcttc gccggggtca atgccgttag cgatctggga
       61 gcgcgcatca tcgcgccgct gccgagccgc cttgagactc acttctggat agacaccgaa
      121 ggaaagcccc ttctcctttc cgccgaaccg atatttccaa cgccaccact tccgcccagt
      181 tggagtgacg atcaaataga gcccaccacc gtcaaacagc ttgaacggag ttgcccgcgg
      241 cttggcggaa cgaatcgcaa tatccgagag cggcatagtt ggggtaactc accttctagg
      301 tctaagagtt gcccccagaa ttaccccagg attggggtag cttcagccgg acagcatcgg
      361 accatgccgg acactatata ttattaagta tttaattata aataactttc gggatggtgc
      421 tggatgaggt tggacactga attggtggag gcggggggaa tcgaaccccc gtccgagagt
      481 cttccatctc cgcgactaca tgcttagccg atgttttagt gtcacccttg gagcccccaa
      541 cggcagggtt tcccagggcc atccggttta gatctcagcc tcatcccctc cggcagagga
      601 atcagcccat cccactgaat gacatcttat ccgatctcgt gggagaagat cggtaagacg
      661 ctaactgcgg ttaagcagct agttggtatt cgaaattatc tgcgtttaaa ttgcagtctg
      721 tttgtttaac gggacaacag taccccggca tgccactttg acttcccacc cccgtcgaac
      781 ccacatcgcc cccgtacaac tatattaccg tttttagccg tcggctgcga tagaccgtca
      841 aacaaaggtt caagctcttt atctgagtta tcttgcctta agagagcgct ccacttcgcg
      901 ttcagcgact ttgcggcgca atgtctcgcg cttatcataa agcttcttgc cgcgcgccaa
      961 acccaactcg acttttgcct tgccgttttt gaaatataat ctcaaaggaa ccagcgtcaa
     1021 gcctcgttcc gtggttttac cggtcagtct ttctatttcg cgcgcatgta gaagcagctt
     1081 gcgggtccgc gtcggctcat gattaaactg gttagctcct gggtaatggc tgacatgagc
     1141 attgactaag aaggcttcgc ccttctgaat tcgcgcgtaa ctgtccgtca ggtttgcctt
     1201 accgtcacgc agggacttca cctcgctgcc caacaacatc agaccggcct cataggtctc
     1261 ctctatgaaa taattgtggc gggcctgccg gttgacgcag acgttatgtt cgcctgtcac
     1321 cttttcctta gtcacgccgc ctgctcctgt cgtctggagt aatcaagcca ccggaagtga
     1381 tgagcttgag ggcttcttcc atcgtcatgt cgagcggcga tatttcggtc gtcggcacaa
     1441 ggagatagaa gccggacgtc ggattagggg tggtcggaat gaaaacgttg atgagctgag
     1501 catcttgctt cctttcaagc tcccgccatg cgcggcccat ggcaaatccg acagtataga
     1561 tacccttgcg tgggtactcg atcatcacta cctggcgcga gttctgtgat tgtcccaaaa
     1621 aagtttggac aagtttctgg accgcggtgt agacaccacg gaatatcggg atctttacga
     1681 aaacattttc ccaggccgcg agaaacctgc gagtggccac gcctcgggta agaattccga
     1741 gaaataagat tacagtcacg gttacgagcg cgcccaatcc ggggattgca aaaggcagat
     1801 aggtattggg atggacgccc gccggtaaga catcaagcac tccatctaac aggccgataa
     1861 cccatccaag aaaccacaac gtgatcacta tcggcagaaa taccaacagc ccggccaaaa
     1921 aataacgttg gaagttctcg cacaaccgaa ctctattctt ggctggtacg tcttccccca
     1981 ccctattctc ctggagatcg caacaaaact gcatcatcga ttaagcgcgc tttaccgatg
     2041 gtaaccgaca acgctagaag cgccgaatga cgaatttgat cgagcggttc taaggtttcg
     2101 gcatcgcaga tggcgacgta ctctaccacg gccaagggtt cagcttgcag ctcggctatc
     2161 accacttcga cgattgtttt cgccgacacc tctccctgac ggaccaggca ttcggcttta
     2221 ctgagggagc gcgacaaaca gcgcgcagcc tttctctggt cagcattaag gtacgcgttg
     2281 cgagaactca tggccacgcc atcgctttca cgcacaattg gatggccgac aatttcaatg
     2341 tctagtaaga gatcgcgcac caaacgccta atgacctgca gttgctggta atccttttct
     2401 ccaaaaatag cgacgtcagg acgaacaaca ttgaaaagct tggtcacgac ggtagccaca
     2461 ccccggaagt gacccggccg ctgcaaaccg cataggatct tactaagctg ttgaacttca
     2521 acatgcgttt gattacccag agggtagatt tcttcagccg aaggatgaaa gagtacgtct
     2581 atttcttcta tctcaagcaa actgcaatcg cgagcaaagt ctcggggata agcggcgaaa
     2641 tcttcagccg gagcaaactg cgtcggatta acaaagatcg agaccacgac ccgatctcct
     2701 ctcctcttgg cctcacgtac caggcttaag tgcccctcat ggagagcgcc cattgtcggc
     2761 acaaaagcaa tacggtggcc ttgctgccgc tcagcttggc tccaatccgt catctctgtc
     2821 gtgttccgga ttatgcgcat tcgataacga tccgccgcga aatcccgaat acacggcttg
     2881 atcggttctt cacttaaacg aatgttcctc accaggaaat ttacgctccc gaacttcgtc
     2941 gacgaaattc tttaccgcgg cagtcatcga gccttttatg tcggcatagc gtttgacgaa
     3001 cttgggggtg aaatcgtcga agagaccgag catgtcgtgg agtactagaa cctgaccgtc
     3061 gcagtgaata ccggcgccga tcccaatggt gggaatggtt acccgttcgg taatttcctt
     3121 tgccaatgcc gttggcaccc cttcaacgac gatggcgaag gcgcccgcct cttggacggc
     3181 taaagcatca cgcacaataa tatcgtattg tcctttttcc cgcccctgta ccttgtaacc
     3241 gccgaaccga tgcaccgatt ggggcgttaa cccgacgtgt cccatcactg gaatcccggc
     3301 gttgacaatc gccgcgatgg tgtcctccat ggctattccg ccttcaagct tgatcgcttc
     3361 cgcgccagct tcctgaagaa atcgtccggc attgcggagt gcttgttccc tgccaacctg
     3421 gaaggacaag aagggcatat cgccgaccac tagagcgcgc ttgcaacccc gcgctactat
     3481 ccgagtatga tagatcatct catccatggt cacggctaaa gtattgggtt gcccttgaac
     3541 aacactgccg acagagtccc ccaccaatat aacgtccacg ccagcatcat cgaggattcg
     3601 cgcgagcgag tagtcgtaag ccgtaaggca agtgattttt tttccttggt gcttcatctt
     3661 gacgatgccg ggcacagtga ccttttccgc catgggttcc ctcccgtaaa aaaaaagcct
     3721 tccagacaag ccggaaggcc aatataccga tgagcctgcg aaaggctctc gcgctgctga
     3781 ctcgatgttg gcatccgtct cagtccaact atctggatcc aagcggtata aaatgctgtg
     3841 ttcccgaacc catattgttg atttccttga tcaagtcagc cagatccttg ctgctctcaa
     3901 cgaaatcaat tttcgacgtg tttaccacca gaagtggtgt ttcgtcgtaa tggaaaaaga
     3961 actgattata agcctgggca acctcgttca agtattcgaa agttacgcca cgttcgtatt
     4021 ttttatccct ttttttgact ctcttgtaaa gtacttcggg acgggcctgc aggtagacca
     4081 ccaggtcggg tttgggcacc cgggcattca aaagagagta gatctgctga tacaaattta
     4141 gttcttccgc gctcaaggtg agagtggcaa agattctatc tttagcgaac agatagtcag
     4201 cgatgacatt ctgattgaaa agatcctgct ggcttaactc aatctgttgc tggtaccggt
     4261 taagcaagaa aaatgtctga ttttgaaacg cataggtttg tcgcgcctga taaaactttg
     4321 gcagaaaggg attgtcttca atgcgctcga acagggatcg agcttgaaac tgattggcga
     4381 gcatcttggc caaactgctc ttgccgacgc cgattggccc ctcgaccaca atgtacttgg
     4441 tattgctcat gcaagtctgg gaatataaca caatcgatca aaaatcgtcc actgtatgct
     4501 acacttgcga tggtgaaaga actcgagatc aaaatcgg
//
LOCUS       DASUTG010000801         7458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_418879, whole genome shotgun sequence.
ACCESSION   DASUTG010000801 DASUTG010000000
VERSION     DASUTG010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418879"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(792..1784)
                     /locus_tag="VF089_18595"
     CDS             complement(792..1784)
                     /locus_tag="VF089_18595"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176026.1"
                     /translation="MIQPKWTAIFLGVVTLTLVALVAEAQQPKKLWRIGFLSSMSPGA
                     YAHLYTAFHQGLRDLGYVDGQNVTIKTRWAEGNTERLPELAAELLGHQVDLIVSTGGI
                     LTALAAKAATTHTPVVFTAGGDLIRVGLISSLARPGGNLTGLNLLTTELGIKRLELLK
                     ETFPKIRRVGILGNPINPNHTMQVNETQAAAKELGLQIQVLEARNLNEVETVFSKLSE
                     KSVSALLMLPDSMLNAHRERSAELATKSRVPAIFEFKEFVEAGGLMSYGTNIVAVYRR
                     VPVYIDKIVKGAKPAELPVEQPTNFEFVINLKSAKQMGLIIPPNVLARADRVIR"
     gene            2160..3281
                     /locus_tag="VF089_18600"
     CDS             2160..3281
                     /locus_tag="VF089_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6176027.1"
                     /translation="MNRQWLGTAFFFALLLLILYFALLILTPFLKAIAWAVILAILVY
                     PVYAWLLKLLRWRATPAALIVIVAITLLVIIPGIELGWFLTDEAITLVQTVRSLMSDD
                     GLARWAETPWVQSILIWWDVISFRLIDFKINWKEILAQGAQVSSGFIVSQVKGVAQNI
                     LLVIVNFIIALFTLFFLLRDGKDFFRRIQRLLPMDREHQERLFKNVVDAVLAVVHGSF
                     VVAVIQGLLAGLAYWVTGVPFAALWGVVTAFFALLPVGGSTLVSIPVCLYLFFQGEML
                     RGFLLMAWSLGVVGTVDNVLKPLLIGNRLGLPVLLLFFGILGGIALFGALGIVLGPVI
                     FALLRALLDLYSEEYVEEERQRLTYPASLADNQRAEDQL"
     gene            3519..4010
                     /locus_tag="VF089_18605"
     CDS             3519..4010
                     /locus_tag="VF089_18605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3702..3704,aa:Sec)
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX6176028.1"
                     /translation="MTDDMEVKREIVLEADAETIERMRKEGHARGFTVYCDEAERIGG
                     DNSAPPPLAYFGLAVGFULLTQISRYGEMLKTHIDKARVLVTARFKNEGSVLRETVRA
                     EGLGVETRCEITSTEPAEKIAKVLRNAEAGCYVIQTIRNPTEVKSQYTLNGATFEPNS
                     YNK"
     gene            4039..4284
                     /locus_tag="VF089_18610"
     CDS             4039..4284
                     /locus_tag="VF089_18610"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6176029.1"
                     /translation="MAKEAEFFNVKYREGSLDAKTAQLILMAVNFAIGHEHGAKLHLS
                     KARENGATEDEIQEAVVYCMRPVAAKVRNFAKEIIAK"
     gene            4317..5171
                     /locus_tag="VF089_18615"
     CDS             4317..5171
                     /locus_tag="VF089_18615"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HEX6176030.1"
                     /translation="MNDWTIEAGEENFETAVLERSHEVPVVVDFWAPWCGPCRVLGPV
                     LEKLADEYSGKFVLAKVNVDESPSLAGAFGIQGIPAVKLIKDGEIAGEFTGALPEPAV
                     REMLSRYLPSEFDEQADEAADLEEQGKTAEAQAIYQSILDAEPNHAKSLLGLGRVLMN
                     AGDRDGALETLERISPAAEERKAADRLIARLQLQGDQSADEATLREKLAAEPDSLEAR
                     FDLAQVLAAKEKFEEALNEFLKIVKSDRGFRDDGARKAMVQIFDVLSPGDPLIDKYRS
                     ELAKILFR"
     gene            5522..>7458
                     /locus_tag="VF089_18620"
     CDS             5522..>7458
                     /locus_tag="VF089_18620"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX6176031.1"
                     /translation="MVTAADKIDREVKRQARALYMRAAEHKPSIFEGRDLLGRMIDWS
                     LEDDSLRTALFRFVDVLPSLQSSAEIGRHLEEYFARVDHALGGLVYVAQALQAGRWVA
                     PVVRRNVVSFARRFIAEENTDQLISIVSGVRAEPAAFTLDVVGEATVSDSEAVAMENR
                     YLTLLRSLSGPAAAWPLRPQIDESPFGPVPRVNLSVKLSSLCARFDPLDPETESAVRR
                     RVRSILREAGRLGAAVTIDMEQYAFKPLTFQILRALLEEEEFRSTPPVGLAVQAYLRD
                     TESDLADLIGWAKQRVRRIGIRLVKGAYWDSEVAWAQQKNWPVPVFLDKAETDGNFER
                     LSRLLLANNDIVDAAFGSHNLRSLAYAIVTANRLGVPRNGYEIQMLYGMAEPFRRAII
                     ENGQRVRVYLPVGELLPGMSYLIRRLMENTSNTSFLRQTYADKKDVESLIAAPTAAAR
                     NASRLPQAAHPGEISPFRNEPAIDFAHSENRAQFYKALEATGERIKRRAMPARSGEWL
                     ESVNPANLKEVVGRVRIASTGEADDAIAAATRFFPKWRATPAKKRADILLRAAEIMRR
                     KRWELASWEVFEVGKGWREADGDIIEAIDYLEYYAREMIRLAQPRITQQLAGESNVYF
                     YEPRGIAAIIAPWNFPLAILTG"
BASE COUNT         1587 a         1923 c         2145 g         1803 t
ORIGIN      
        1 ccagacgggg agagaaagcc gagacgataa actccacgcg cagtttgcgc cgaggcatca
       61 gagccaggcg cgaacaatgt cgtgcacacc gcaatggcag caatcaaagc ttgcatacaa
      121 ttgctcgttg tctcacgtca cgattatcga atcacccaat ccgctcgcgc cagaagattc
      181 ggcacgattg tttggtatgt gttggctggt gtttcgccct ggagctcgca gatctaccga
      241 ccatcctact gatctgtttt gctccgtgta ggcgatgcca aggataggac aggcgtacgg
      301 caacacaaat tcagagacta acgtcgatgt tgcaaattcg cttctgcgct tccgccatac
      361 ccgcacagct cgcgagcgag aggaggctgc aaaacttcac accggatctc agatagtcgc
      421 tacttcgact tgcctattcc cgattgtcgc ccaagcgagg ccaaccatag tcgatcgtcg
      481 gctttttgtc tcattaattc tatgtcgaag cgcgctggta agtaggcgaa cggtgatgga
      541 aaccgccggc tttttgacgt ttgttcatga taaaaccgct gggatctgat ttatctgatc
      601 accttatccg cccgcgacaa gacgttgggt ggaatccatc taaagaaaat ccgaaatccg
      661 aatatcgaaa tccgaaacaa acgcacgcga aagcggaatt tgagttcccg agcaccgttt
      721 tagagttttg tcccttttgt cgtttcgatt tgtttcgagc ttcggatttc gaatttcggg
      781 tttcaaacag ctcatcgtat gactcgatct gctctagcca ggacattggg tggaatgatc
      841 aatcccatct gcttagcgct ctttaggttg atcacgaatt cgaaattcgt cggttgttcc
      901 acgggcagct ccgctggctt ggcgcctttg acaattttat ctatatagac gggcacgcgg
      961 cggtacacgg ctactatatt agtcccgtag ctcatgagac cgccggcttc gacgaactcc
     1021 ttgaactcga aaattgctgg aactcggctt ttggttgcca gctcggcgct tcgttcgcgg
     1081 tgcgcattga gcatcgaatc aggcagcatg agcagcgcag agacgctctt ttccgagagc
     1141 tttgaaaaca cggtctcgac ctcgttcaga ttccgcgcct ccagaacctg aatctgcagg
     1201 cccagctcct tcgcggcggc ttgagtttcg ttcacttgca tcgtgtgatt tggattgatc
     1261 ggattcccca gaatcccgac gcggcgaatt tttgggaagg tttctttcaa cagctcgaga
     1321 cgcttgatgc cgagctcggt ggtcagcaag ttcaatcccg tgagattacc acctggccgt
     1381 gccaggctgg aaatcaggcc gactctgatg agatcgccgc cggcggtgaa aacaaccggg
     1441 gtgtgggtcg tcgccgcttt tgccgccagc gcggtgagaa tcccgccggt tgaaacgatt
     1501 agatcgactt ggtgtcctag caactccgcg gctaattctg gcagccgttc cgtattgccc
     1561 tcggcccatc gtgttttgat cgtgacgttt tgcccatcca catagccgag gtcgcggagg
     1621 ccctggtgaa acgcggtata caaatgcgcg taggcgcctg gggacattga ggatagaaaa
     1681 ccgatgcgcc agagtttttt tggctgctgt gcctcggcta caagcgcaac cagggtcagt
     1741 gtgacgaccc caagaaaaat cgccgtccat tttggttgaa tcaatcttcg gcttggctgg
     1801 gagcccggcc tgagtgtatc gtagctacga gcgttggctt tgtgcggttc ggatcgaaga
     1861 tcctggactt tgctcttcgg tcggtggccg atgtatgctc gtgctttcac cacaaccctt
     1921 tagcgcaagg gttgtaccag ccgatcgatg atgcaggtta gacctaagtt gctgaaacat
     1981 ttcaaaagaa tcgtgcgctg cgctggttcg cattctggag acagtagtcg atttgctacg
     2041 gcgcggtagc aaagttgctc cgtcgcaact ggctgactgc gcctgcgtcc aacgtaggtt
     2101 gcagccattg aaccttgtgc gtagagttgg ctagccaagt tcaccggtag ggggtttgtg
     2161 tgaatcgtca atggttgggc acggcgtttt tcttcgcgct gcttctgctc atactgtact
     2221 tcgctttgct gattcttacg ccgtttttga aggccattgc ctgggccgtg atactggcga
     2281 tcctggttta tccggtctac gcctggctgc tcaaactgtt gcgctggcgc gccactccgg
     2341 cggcgttgat tgtaatcgtt gcgattacgc tgctcgtgat cattccggga attgagctcg
     2401 gctggttttt gaccgacgag gcaatcacat tggtgcagac ggtgcgttct ctgatgagcg
     2461 atgacggtct cgcccggtgg gcagagacgc catgggtgca gagcatccta atctggtggg
     2521 atgtgatctc cttccggctc atcgatttca agattaattg gaaagagatt ttggcccaag
     2581 gcgcgcaggt atcctcgggt ttcatcgttt cccaggtaaa aggcgtcgcg cagaacatct
     2641 tgctcgtcat tgtcaatttc atcattgcgc tgttcacact ttttttcttg ctccgtgatg
     2701 gcaaagattt cttccgccgt atccagaggc tattgccgat ggaccgtgag catcaagagc
     2761 gcttgttcaa aaacgttgtc gacgcggttc ttgcggtggt ccatggctcg tttgtagttg
     2821 ccgtcatcca gggactactc gccggattgg cctactgggt gacaggggtt ccgtttgccg
     2881 cattgtgggg agtagtcacg gcattttttg cgctgctgcc ggttggcggc tcgaccttgg
     2941 tttcgattcc ggtctgcctt tatctttttt ttcagggcga aatgctgcga ggttttttac
     3001 tcatggcctg gtctctgggg gtggttggaa ctgtcgataa cgttctaaag ccgctgctaa
     3061 tcggtaatcg cctgggtctt ccggttttac ttcttttctt tggcattcta ggcggcattg
     3121 cgctgttcgg cgcgttggga attgtcttgg gcccggttat ctttgctctg ttacgagctt
     3181 tgctggatct ttactccgaa gagtatgtag aagaggagag acagcggctg acctaccctg
     3241 catctttggc tgacaaccag cgagcagagg accaactctg aagttggcat tggcgctcga
     3301 gacgccttct acgtgcctaa cggactggcg gcaattgagc agcgcaagaa tagctgtgat
     3361 cattgaagct gtgcgttccg tgcttcactc tctgtgttcg caatttagcg accgctgttg
     3421 cggattgatt agacatttgg aatggcatca ctggtaagtt gcaataaccg ctgaaatgca
     3481 cttatgttgc ggacgatctt aatttgaagg ggatagatat gactgatgat atggaagtga
     3541 aaagggaaat cgttttggaa gcggatgccg aaaccatcga gcggatgcgc aaagaaggtc
     3601 atgcgcgagg ctttaccgtc tactgcgacg aagccgagcg gatcggcggt gacaactcgg
     3661 cgcctccgcc actcgcgtac ttcgggcttg cagttggatt ctgactgctc acgcagattt
     3721 cccggtacgg ggaaatgttg aaaacgcaca tcgataaagc cagggtcttg gtcaccgccc
     3781 gctttaagaa cgaaggttcg gtgttacgag aaacggttcg ggccgagggc ctcggtgtag
     3841 agacccgttg cgagattact tccacagagc ccgccgaaaa aatcgccaaa gtcctccgca
     3901 atgctgaggc gggatgctac gtgattcaaa cgattcgcaa tccaacagag gtcaaaagcc
     3961 agtacacact aaacggcgcg acgtttgagc cgaattccta caataaatga acgcaacaaa
     4021 aacataggga ggtctgtcat ggccaaagaa gcggagttct ttaatgtaaa atatcgggag
     4081 ggatctctcg acgcgaaaac cgcacagctg atcttaatgg cggtgaactt tgctatcggg
     4141 cacgaacacg gcgccaagct tcacttgtcc aaggcgcgcg agaatggcgc taccgaagat
     4201 gaaatacagg aagcggttgt ctactgtatg cgtccggtgg cagcgaaggt gcgcaatttc
     4261 gctaaagaga tcatcgccaa gtagatcttg tcgcacgaat cgattacctt gagcctatga
     4321 acgattggac cattgaagcc ggcgaggaga actttgagac tgccgtgctg gagcgttctc
     4381 atgaggttcc ggttgtggtt gatttctggg cgccctggtg cggtccgtgt cgggtattag
     4441 gccccgtgct cgagaagttg gcggacgaat actccggcaa gttcgtgctc gccaaggtta
     4501 atgtcgatga gtctccgtca cttgccggcg ccttcggcat tcagggcatc ccggcagtga
     4561 agttgattaa ggatggagag atcgccggtg agttcaccgg cgcgttaccg gagccggccg
     4621 tgcgcgagat gctgtcgaga tatttgcctt ccgagttcga tgagcaggcc gacgaggcag
     4681 ccgacctgga ggagcaagga aaaactgccg aggcccaggc gatttaccag tccattcttg
     4741 atgccgaacc gaatcacgca aaatcattgc tcggtttagg ccgcgtcctg atgaatgcag
     4801 gcgatcgcga tggcgcactt gagactctgg aaagaatctc cccagccgcc gaagaacgaa
     4861 aggccgccga tcgtctgatc gcgcgattac aattgcaagg cgatcagagt gcggatgaag
     4921 cgacattgag ggagaagctg gccgcggaac cggacagctt agaggcgcgc ttcgatcttg
     4981 ctcaagtctt ggccgctaag gaaaaattcg aagaggctct caatgagttt cttaagattg
     5041 tgaaaagcga tcgcggcttc cgcgacgacg gtgcgcgcaa agcgatggtg cagattttcg
     5101 atgttttatc gccgggtgac ccgctgatcg ataagtatcg ctcggagctc gccaaaattc
     5161 tgttccgtta acagactgct gaaaagaccc atagtcttcg ttgcgctcaa tcgcctcgcg
     5221 tcaacgtact ggaaagcacg tctccgctcg tcgattttcg tgcgcctcgc ctttgggatc
     5281 ttttgagcag cctgttaaca ttcagcaact tgcgaattcc aatgtcctgc taagaagttt
     5341 gttgataatg cggtcggttg ttagctgcca aattgacgat gactcttaag agcctcacag
     5401 gtaggaaaaa gcggtagaat aaacgggaga acagtagagt tattctcgcg tgtccttttc
     5461 tagggagcca tgtgactccg gaatctgctc ttgggatctt acaaaggcga aactgaatca
     5521 aatggtaacg gcggcggaca aaatcgatcg ggaggtcaag cgtcaggctc gcgcgcttta
     5581 catgcgtgcc gccgagcaca agccatccat cttcgaaggc cgcgatctat tgggccgaat
     5641 gatcgattgg tcgctggaag atgattcgct gcgcactgct ttgttccgct tcgtcgatgt
     5701 gttgccgagt cttcaatctt cggcggaaat cggccggcat ttggaagaat acttcgcccg
     5761 ggtggatcat gcattgggcg ggctggttta tgtcgctcag gcgctccagg cgggtcgatg
     5821 ggttgccccg gtggtgcgcc gcaatgttgt gagtttcgcg cgccgcttta ttgccgaaga
     5881 aaatactgac caactaattt cgattgtctc cggggtcaga gcagaacctg cggcctttac
     5941 cctggacgtg gtcggcgagg ccacagtcag cgacagcgaa gccgtcgcga tggagaaccg
     6001 ctatctgact ttgctccgca gtttatcggg gcccgcggcg gcatggccgt tgcggccgca
     6061 aatcgatgag tcgccgtttg gaccggtccc gcgggtcaat ctttcagtca agctttcttc
     6121 actgtgcgcc cgcttcgatc cgctcgatcc cgagactgaa tccgcggtgc gccggcgggt
     6181 acgttcgatc ctgcgcgaag cagggcggct cggtgccgca gtaacgattg atatggaaca
     6241 gtacgcgttc aagccgctta cgtttcaaat actccgggcg ctccttgaag aagaagagtt
     6301 tagatcgacg cccccggtcg ggctggcggt tcaagcgtat ctgcgcgaca ccgagagcga
     6361 tctggccgat ctgattggct gggccaaaca gcgcgtccgg cgcatcggca ttcgattggt
     6421 taaaggagca tactgggaca gcgaggtagc atgggcgcag cagaaaaatt ggcccgttcc
     6481 ggtattttta gataaggcgg agactgatgg caattttgaa cggcttagcc gcttgttgct
     6541 ggcaaacaac gacatcgtcg atgccgcttt cggcagccat aacctccgga gtctcgccta
     6601 cgcgattgtc acagccaatc gcttgggcgt gccgcgcaat ggctatgaaa ttcaaatgct
     6661 ctacggcatg gccgaaccgt ttcgccgggc gatcatcgaa aatggccaga gggttcgtgt
     6721 ctatcttccg gttggcgagc tgcttccggg gatgtcctat ctgatccgcc ggttgatgga
     6781 gaacacttcg aatacttctt tcctccggca gacctacgcc gacaagaagg acgttgagag
     6841 tctgatcgcc gccccgactg cggcggctcg aaatgcttcg cgccttccgc aagcggctca
     6901 tccgggcgaa atttcgccct ttcgcaatga gccggcaatc gatttcgcgc attcggagaa
     6961 ccgcgcccaa ttttacaagg cgctggaggc caccggcgag cggatcaaac gtcgggccat
     7021 gccggcgcga agcggtgagt ggttagaatc ggtcaatccg gccaatctaa aagaagtcgt
     7081 tggccgggta aggatagcca gcacaggaga ggccgacgat gctatcgctg cggcaactcg
     7141 cttcttcccg aagtggcgcg ctacgcctgc aaaaaaacgc gcggacattc tcctgcgtgc
     7201 cgcggaaatc atgcgccgca aacgctggga gcttgcatcg tgggaagttt ttgaagtcgg
     7261 caagggctgg cgtgaagcag acggcgatat catcgaagcc atcgattatc tcgaatacta
     7321 cgcccgcgag atgatccggt tggcccagcc ccggatcacc caacagctcg ccggcgagag
     7381 caatgtttat ttctatgaac ccagaggcat cgcagccatt attgcgcctt ggaattttcc
     7441 gttggcgatt cttaccgg
//
LOCUS       DASUTG010000802        10686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2199724, whole genome shotgun sequence.
ACCESSION   DASUTG010000802 DASUTG010000000
VERSION     DASUTG010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199724"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF089_18625"
     CDS             <1..677
                     /locus_tag="VF089_18625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6176032.1"
                     /translation="RCRRFNVDLADLIQEGNIGLMRAAEKFDHRLGCRFSTYATWWIR
                     QFITRSMVDSAHMIRIPAHIVEARSKLLRGFQGLAQKLGRTPLAEDIAAETGLSPQEI
                     RKLITLPGEHLSLQAPLGDDPQETLQHYVRDRTAEDPLEELSKKDLCSVTRKSLSILN
                     SRQEVVLRHRFGIDLTREHTLQEIGDMFLITRERVRQIEAKALRQLRMPSAKKKRKRT
                     AGALSV"
     gene            830..1135
                     /locus_tag="VF089_18630"
     CDS             830..1135
                     /locus_tag="VF089_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176033.1"
                     /translation="MYVSRLTFVTLPGKTGELEHELKTLRDLVKQVGGENTRIMRTHF
                     ASADAPNVVFKQDAPELKTLEEQIRKVTAEPSFQEWSKKVSPLLRQTPNREIYMVVD"
     gene            complement(1208..2254)
                     /locus_tag="VF089_18635"
     CDS             complement(1208..2254)
                     /locus_tag="VF089_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6176034.1"
                     /translation="MRRSNVWAIVLAGGEGTRVRGFLKQLCGGSGLKQFSPIIGRRSM
                     LQHTLDRMGQLVSPERTLIVVGAHQSAEAARQLAHWPQENIIYQPANRDTAPGILLPL
                     SYITHWDPSSTIIVSPSDHFVRDENRFMKSVASALADLKGHSDKMILLGMTPEQGQET
                     EYGYIQIKKHSRATRTRPVAGFVEKPPLMLARRLIDQGALWNTMVFAVRSTTLWQMVR
                     DTVPSLYNAFGLIQSMLGSIHAPGFIQHIYQTIPNVNFSSDICAPLAKKLRVLKVPNV
                     GWSDWGTVASILRSLIEMGQLETFRMRLAESQVHPSWYAASLAEIDPQSKDLPAEAVY
                     SSLRSEVEVFLSPD"
     gene            complement(2504..2794)
                     /locus_tag="VF089_18640"
     CDS             complement(2504..2794)
                     /locus_tag="VF089_18640"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX6176035.1"
                     /translation="MPIVRMIFVRVVPQEAKNAKQIWTESCAPLMIQQPGCLSEKLLR
                     STDDPGEFISYSEWKDQQSIDAYRVSEAHEEIKKHARHLHGDRPSVKRYEVV"
     gene            3101..3583
                     /locus_tag="VF089_18645"
     CDS             3101..3583
                     /locus_tag="VF089_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176036.1"
                     /translation="MKQAFGSGDTGEFSGSTRFESKDADFSDDILSQISSMPMGIHAA
                     SNQNRRMEVMGEFILVQAILREAIRTYQKYAVKKGTRASRLFRDVNEWFLSDDRRWFF
                     SFVNVCDTLGLEPTYIRTGLKLWRERKLQANGVSPAVPAYQPVPRNSRQRLAANELRR
                     "
     gene            3897..4022
                     /locus_tag="VF089_18650"
     CDS             3897..4022
                     /locus_tag="VF089_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176037.1"
                     /translation="MIPPIGLALIALGVVLAIWGGIAGWWMTRGGDVHVGGPGKR"
     gene            4292..5644
                     /locus_tag="VF089_18655"
     CDS             4292..5644
                     /locus_tag="VF089_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6176038.1"
                     /translation="MSATILVCDDDSVPRRNIASVLEEEGYTVHQAEDGKAAIDAVNQ
                     IDFDLVLTDLKMPGVDGLSVLKHVRSVSPQTLVILMTAFSSVDTAVEAFRLGAQDYIL
                     KPIVIEDVIRKVGRFLDHKNLVWEVEMLRREINRSSEPTSLVGTSKPIQDIFKIVSKV
                     AATNSTVLITGESGVGKEVVARTLHLQSLSKDKVFLPVNCSAIPDTLLESQLFGHAKG
                     AFTGAINAQEGLFQKAQGGTIFLDEIGEMPLTLQPKLLRAIETKEVLAVGSTQPVRLN
                     VRIIASTNRDLEREAGEGKFREDLFYRLNVVHVWVPPLRERREDIPLLVDYLISRHNR
                     EMKKAYKGADNSAMKVLMSLPWKGNIRELDNVLERAMILGDGEWITPEHLPESEIPEN
                     DRNVTANNLRVAVESYEKSHIANVLRDTAGDRTRAAELLGLSRSSLYRKMEKLNIRDD
                     "
     gene            5853..7931
                     /locus_tag="VF089_18660"
     CDS             5853..7931
                     /locus_tag="VF089_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX6176039.1"
                     /translation="MTRNDPGTRSRHKPNSDTASRTRNLPAGSASASSAEPRIRAVIE
                     GVKPEIDCGRFPIKRVVGDRVTVEADIFADGHDAMSARLLFREQGETDWREAPMELLV
                     NDRWRGAFKITALTDYFYTLEAWVDRFKSWRDGLSKKAAAKQDVHLELLEGAELIALA
                     AKRAGGGDAEKLTAYAAALRAKEAGAIEQALDEELAVLMSKHAERRWPARYDKELRIV
                     VDRPIAEFSSWYEMFPRSCADQPRKHGTLRDCMARLPSIAELGFSVLYLPPIHPIGRS
                     YRKGINGSLVAGPDDVGSPWAIGSEEGGHKSVHPLLGSLADFKQLVEKAREHELEIAL
                     DIAYQCSPDHLYVREHPQWFRKRPDGTIQYAENPPKKYQDIYPIDFETEDWRALWDEL
                     KSVLLFWCEQGVRIFRVDNPHTKAFRFWEWAIAGVKKDYPEVIFLAEAFTRPKVMYRL
                     AKLGFTQSYTYFTWRNTKAELTQYFTELTRTDVAEFFRPNVWPNTPDILPEYLQAGGR
                     PAFMSRLILAATLGANYGIYGPAFELCVNTAREPGSEEYLDSEKYQIKEWNIESAGSL
                     RELMARINQIRAANPALHHNRSLRFYTVDNDEIICFAKHSEDRSNAIVVAVNLDPHHT
                     RSGWVDLPLAELGLDPAKPFQMHDLLTDARYLWNGPRNYLSLNPASVPAHIFRVRHNL
                     RREQDFDYFM"
     gene            8097..>10686
                     /gene="treS"
                     /locus_tag="VF089_18665"
     CDS             8097..>10686
                     /gene="treS"
                     /locus_tag="VF089_18665"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX6176040.1"
                     /translation="MSRKPAKSTSTFLDDNPLWYQDAVIYEVHVRAFHDGNGDGIGDF
                     QGLTEKLDYLQDLGVTALWLLPFYVSPLKDDGYDIADYTQIHPDYGTLHDFRLFLRGA
                     HERGLRVITELVINHTSDQHPWFLRARDATSGSSARNFYVWSDTQERYPEARIIFKDF
                     EPSNWSWDSVAKAYYWHRFYSHQPDLNFDNPEVHKAIFDALDFWLDMGVDGLRLDAVP
                     YLYEREGTNCENLAETHAFLKKLRQHVDSKYKNRMLLAEANQWPEDAVAYFGNGDECH
                     MAFHFPVMPRLFMSVYMEDRFPIIDILDQTPAIPESSQWALFLRNHDELTLEMVTDED
                     RDYMYKVYASDPRARINLGIRRRLAPLLGNNRRRVELLNSLLFSLPGTPVIYYGDEIG
                     MGDNIYLGDRNGVRTPMQWSADRNAGFSRANPQRLYLPVIIDPEHHYETVNVEAQQQN
                     PGSLLWWMKRLVLLRKNYQAFGRGSIEFLLPDNRKMLVFVRRLGDEIILVVANLSRFV
                     NYVELDLSAYKGMVPVELFGHTRFPSIGELTYFLTLGPHAFYWFKLEQTRVSEARAGA
                     EGYELPSLEVEGSWQAVLEGRPRTMLERSLPAYLLTCRWFGGKAQQIRSVKNLDVIPF
                     PVDSATIYLTTWAVDYIGGTPETYLLPLAYASGDRAFELRQSASQAIIAQLKIEDEAG
                     ESEGILYDAVHEPGFLKSLLTALARGRRFKDTGAELLAHPSKGFRELMGTPETALEPL
                     LIKREQSNTSVAYGDRLMFKLFRRVTEGLNPDVEIGRFLTELEFAHVPALAGHLELRK
                     GRGEPATVGILQRLVANEGDAWRYTQDSLGYYFEQVLTRQVELGEASLPNASPVELTQ
                     HETPELA"
BASE COUNT         2443 a         2832 c         2968 g         2443 t
ORIGIN      
        1 aacgttgccg ccgtttcaac gtggatctcg cagatctgat tcaggaggga aacatcggtc
       61 tgatgcgcgc cgccgagaag ttcgaccatc gcctgggatg tcgtttctcc acttatgcga
      121 cttggtggat acgccaattc atcactcgca gcatggttga ttccgcacac atgattcgga
      181 ttcccgcgca catcgtcgaa gccagaagca agttgctgcg cggtttccaa ggccttgcgc
      241 aaaaactcgg caggacccct ttggcggaag atattgccgc cgagaccggc ctaagccctc
      301 aggaaattcg aaaactcata acactgcccg gcgagcatct gtcattgcag gcgccgcttg
      361 gcgatgaccc ccaggaaacg ctgcagcatt acgtaaggga tcgaacggct gaagaccctt
      421 tggaagaact ttcaaagaag gacctttgtt ccgtcaccag aaagtcatta tcgattctca
      481 attcgcggca agaagttgtg ttgcgccacc ggttcggcat cgatcttact cgagagcaca
      541 ccttgcagga gattggtgac atgtttttga tcactcgtga gcgggtgcgg cagattgaag
      601 ctaaggcgct gcgccagctg cgcatgccgt cagccaagaa aaagcgaaaa aggaccgcgg
      661 gcgcgctttc agtttagtag ccggatgaat ttgcttagcg gaaagcaatt tcgggcaaag
      721 gcaatttcta gtatccggaa tccgagatag agaattaact ttcactggcc aggtgaactc
      781 gtaaacagca gattatgagc gggataaaca agcaaaggag gaccttgaga tgtacgtatc
      841 gcgtctaaca tttgttacgt tgccgggcaa aaccggtgaa ttggagcatg aactaaaaac
      901 tttgcgtgac ctcgtcaaac aggtgggtgg cgagaatact agaattatgc gcactcattt
      961 cgcctcagcg gacgcgccaa atgtggtgtt caagcaagat gcgcctgagt tgaagacatt
     1021 agaagaacaa atacgcaaag tgacagccga gccgagcttc caggaatgga gcaagaaggt
     1081 ttcgccgctg ctacgtcaaa ctccgaaccg tgaaatatac atggtagtgg attaaacatc
     1141 ctttccggac cggcggtgga gatcggtcgc cgctgtttga tcatcggaaa caatcgcttg
     1201 ctatggctca atccggagat aaaaaaacct ccacttcgct gcgtagggaa gaatatacgg
     1261 cttcggccgg gaggtccttt gattgtggat cgatttcagc caacgaagcg gcataccagg
     1321 aagggtgtac ctggctctcc gcaaggcgca tgcgaaatgt ttcgagctga cccatttcga
     1381 tcagcgaacg caggatactg gcaactgtcc cccagtcgct ccatcccaca ttgggtacct
     1441 ttagaacgcg tagcttcttg gccagggggg cgcagatgtc agatgagaaa ttgacgttcg
     1501 ggatggtctg atagatgtgc tggataaagc caggcgcatg gatactaccc aacatcgact
     1561 gaatcaaacc aaaggcattg tacagtgatg gcacggtgtc gcgaaccatc tgccataagg
     1621 tcgtacttct cacggcgaaa accatggtgt tccacaatgc gccttggtcg attagacgcc
     1681 gggcaagcat tagtggaggc ttttcaacaa aacccgctac cggccgcgtg cgagtcgccc
     1741 gagaatgctt tttaatttgg atataaccat actccgtttc ctggccctgc tcgggcgtca
     1801 tacctagcag aatcattttg tcggaatgtc ccttcagatc cgcaagcgca ctggccaccg
     1861 atttcatgaa ccggttctca tcgcgcacga agtgatcgga aggggaaact ataatggtgg
     1921 aggacgggtc ccagtgagtg atatatgaaa gcggcaacaa gatgcccggc gcagtgtcgc
     1981 ggttggctgg ttgatagatg atgttctctt gcggccaatg ggcgagctgg cgcgctgctt
     2041 ctgcactctg atgcgcgccc acgacgatga gcgtccgttc gggcgacacc agttgcccca
     2101 tgcgatcgag ggtgtgttgc agcattgagc gccgaccgat gatcggcgaa aactgtttca
     2161 gtccggaccc gccgcaaagc tgtttgagaa aaccgcgaac tctcgtgcct tcaccgccgg
     2221 ctagaacgat cgcccaaaca ttagatcggc gcactcacaa gctccttggc cgcgcacggt
     2281 tgatgaatgt agcgggcagc aagggcaacc ttcgtgccac cttgtatcgc gagaaaactt
     2341 cggctaggtc tggccgatcc gtggcaattc gtgccgagcg cgggacagcg cctcagttca
     2401 ggtttcgcgc aggcgacaca ggacagcggt gctcgcggtg attgcctgcg cgcctatgag
     2461 gtacgcagct gtacttgatt cgacaacgtg actggcccct cggctacacg acttcataac
     2521 gtttaaccga cggtcggtcg ccgtgcagat gtcgcgcatg tttttttatt tcttcgtgag
     2581 cttcactgac tctgtaagca tcgatgcttt gttgatcctt ccactccgaa taagaaatga
     2641 attctcccgg gtcatcagtt gacctgagca gtttctctga aaggcaacca ggctgttgaa
     2701 tcatcaacgg cgcgcaactc tccgtccaaa tctgttttgc gttctttgct tcctggggca
     2761 cgactctgac aaaaatcatc cgaacgatcg gcatgatcaa ttcctccggc tttttaatcc
     2821 taataaacaa caattcccat gccatgtatc acgcagaaag cggatgatac tgcagtcgcg
     2881 cgcctcgccc tgagttcgtg tttcttctgc atacagcagc gaacgctgtc gctcctcgaa
     2941 cgacaatagt gagcgtccca ggaaaatggc tatgtgatag atgttgtccc acattggaga
     3001 atcgcacata gtcaggctgt gcaattccac ccagttaaag aagtggcatg tacgttgctc
     3061 gcatagcaag cgagatcaaa ctggtgtgga ggatcaagta atgaaacagg cattcggaag
     3121 tggcgacacg ggagaatttt ccggcagcac tcgctttgaa tctaaagatg cggatttttc
     3181 cgatgatatc ttatctcaga tcagcagcat gccaatgggc atccatgcag cgagtaatca
     3241 gaaccgtcga atggaagtca tgggagagtt catcctggtg caggcgatac tgcgtgaagc
     3301 cattcgaacc tatcaaaagt acgccgtaaa aaaaggtact cgcgcatcgc ggctgtttcg
     3361 cgatgtcaat gaatggtttt tatcggatga ccggcgttgg ttcttctcat tcgtcaatgt
     3421 ctgcgacaca cttggcctcg agccgactta tatacgcact ggccttaagc tatggcgcga
     3481 gcgtaagctg caagccaatg gtgtgtctcc ggctgttccg gcatatcagc cggtgcccag
     3541 aaattcgcgc cagcggctgg cggccaatga gctgagacgt taacgcaaca gatgtttgcc
     3601 ttgacccggc ccgtttcggc acgaccggat tgctcaaacc caaccgggtc acctcggaaa
     3661 tgtcatctac cgaacaagaa atagagcgat tggtagacga aatcagccgc gctattcaac
     3721 ggagtccggc tgatcaacgg cacgaactgc gttcttacgc gtcgtctttg attagcgaca
     3781 tagctacgga atcgtcggtt cagccgggcc aaactggagc tcaaaccgaa cgacgtccat
     3841 ttggtttagt tgccgccgga ttggctctgc tggtaatagg tgctggattc gctttaatga
     3901 ttccacccat cggattagcg ctcattgccc tcggcgtagt tctcgctatc tggggtggaa
     3961 tagcgggatg gtggatgact cgtggcggcg acgtgcacgt cggcggtccc ggaaagcgct
     4021 aaaccgatac tcacgcctcc tctaagcgcg gcaccaatct tctgcatcgg aaatggctgt
     4081 gttgttttca ggacacgggc ggagtagtgt gtctcaaacg agacacgaaa tccgttttct
     4141 gaattgaaac ttgttatcta ccgcgcggca cgtattttgc gcaatcaata tacgttgcta
     4201 gctttcgtgc ctcgttttag ctatccccaa cgttttcggt gcgggcgcgg ttttgaatca
     4261 atatacatag aaagaacgag tattcactga gatgagcgcg acgatcttag tctgtgacga
     4321 tgattctgta ccgcggcgaa acattgccag cgtgttagag gaagaaggtt ataccgttca
     4381 ccaagccgag gatggcaagg ctgctatcga tgcggtgaat cagatcgatt ttgatcttgt
     4441 tttaactgac ctaaaaatgc cgggtgtcga cggtctcagc gtcttgaagc acgtgcggag
     4501 tgtttcgccc caaacgttgg tgattctgat gaccgccttc tcgtcagttg acacggccgt
     4561 agaagccttt cgtcttggag ctcaggacta cattctcaaa ccgatcgtga ttgaagatgt
     4621 gattcgcaag gtcggccgat ttctcgatca taagaatctt gtctgggaag tcgagatgct
     4681 gcgccgtgag attaaccgtt catcggaacc gacgagccta gtaggcacca gcaagccgat
     4741 tcaggatatt ttcaaaattg tttcgaaagt tgcggcaacc aattcaacgg tcttgatcac
     4801 cggcgaaagc ggtgtcggca aggaagtcgt tgcccgaacc ttgcatctac aaagtctgtc
     4861 caaggacaag gtctttttgc cggttaattg cagcgcgatt cccgacactc tgcttgaaag
     4921 ccagctcttc ggccatgcca aaggcgcatt caccggcgct atcaacgctc aggaggggtt
     4981 atttcaaaag gcgcagggcg gaaccatttt tctcgatgaa atcggtgaga tgccgttaac
     5041 tctacagccc aagctgttgc gtgccatcga aaccaaggaa gttttggccg tgggttcgac
     5101 ccagccagtt cgcttaaacg tgcgcatcat cgcttcgacc aatcgggatc tcgagcggga
     5161 agccggcgaa ggtaaattcc gcgaagatct tttttacagg ttaaatgtcg tgcatgtctg
     5221 ggtgccgccg ttgcgagaac ggcgcgagga cattccgtta ctggtcgatt atttgatttc
     5281 gcgccataac cgggagatga agaaggcgta caagggcgcg gacaactcgg caatgaaagt
     5341 tctgatgtcg cttccgtgga agggcaatat ccgcgagctg gacaacgtac tcgagcgcgc
     5401 catgatttta ggtgacggcg aatggattac gccggagcat ttgcctgaat ctgagattcc
     5461 cgagaacgac cgaaatgtca ctgcaaacaa tttgcgggtc gccgtcgagt cctatgagaa
     5521 aagccacatt gctaatgttc tcagggacac cgccggtgac cggactcgag cagcggagct
     5581 cctcggattg agccgatcct cgctttaccg aaagatggag aagctcaaca tccgtgacga
     5641 ttaatcgact cctgccattc tccagaatag ttgcctggcc agcgaatgca tcactcagag
     5701 tttacagctt tcttaatcaa ctctaatttc cttttacccc gtaaagtgac acccgtgttg
     5761 ataagaatct gcccggacgg taatatcagc gtccagttct gagcacctga gagcgcctcg
     5821 ctaatctagt ctgaaactcc atctagaaaa gcatgacccg caacgaccct ggcactcgca
     5881 gccgccacaa gccaaactcc gatactgctt ctcggacccg caacctaccc gccgggagcg
     5941 cctcagcgag ttcagctgag ccgcgcattc gtgcggtcat cgagggagta aagccggaaa
     6001 tcgactgtgg ccgttttcct atcaaaagag tggtcggcga tcgtgtcacg gtcgaggctg
     6061 atatcttcgc cgatggccac gatgcgatgt cggcgcgcct gctgtttcgt gagcagggcg
     6121 agaccgactg gcgcgaagca ccgatggagc ttctggtgaa tgatcgctgg cgcggcgctt
     6181 tcaagatcac ggcgctcacg gattattttt acacgttgga agcatgggtg gatcggttta
     6241 aatcgtggcg cgacggtttg agcaagaagg ccgccgccaa acaagacgtg cacttggaat
     6301 tgctcgaagg ggctgaactg attgcgcttg ccgccaaacg ggcgggcggc ggcgacgccg
     6361 aaaaactgac agcctatgcg gcagcgctcc gtgccaaaga agccggagcg attgagcaag
     6421 cgctggacga agaacttgcc gttctcatga gcaagcatgc ggagcggcgt tggcccgcgc
     6481 gttatgataa ggagcttcgt atcgtggtcg atcggcccat tgccgagttc agcagctggt
     6541 acgagatgtt tccgcgctcc tgcgctgatc aaccacgcaa gcacggcaca ttgagggatt
     6601 gcatggcgcg cctgccgagc attgctgagt tgggttttag cgtgctttac ctgccgccga
     6661 ttcatccgat tggaaggtca tatcgaaaag gtatcaacgg cagtcttgtg gccggcccgg
     6721 atgacgtcgg cagtccctgg gcgataggat cggaagaggg cggtcataaa tcggttcacc
     6781 cgttgctcgg cagcttggcg gatttcaaac agctggtaga aaaagctcga gagcacgaac
     6841 tcgagattgc cttggacatc gcctatcaat gctcgcccga tcatctctat gtcagggaac
     6901 atccgcagtg gtttcgtaaa cggcctgacg gcaccattca gtatgcggaa aacccgccga
     6961 agaagtatca ggacatttat ccgatagact ttgagactga agactggcgc gctttatggg
     7021 atgaattgaa gagcgtgctg ttattctggt gcgaacaggg cgtgcgaatt tttcgggtcg
     7081 ataatcctca tacgaaagcc ttccggtttt gggaatgggc cattgccggg gtcaagaaag
     7141 actacccgga agtgatattt ctcgccgaag cgtttactcg ccccaaagtc atgtaccggc
     7201 tggccaagct cggtttcact cagtcgtaca cgtatttcac ctggcgcaac accaaagcgg
     7261 agctaaccca gtatttcacc gagctgacac gaaccgatgt tgccgagttc tttcgtccca
     7321 atgtgtggcc gaatactccc gacatcctgc cggagtatct gcaggccggc ggacggccgg
     7381 ccttcatgtc gcgcctgatt cttgccgcca cattaggtgc caactacggg atctatggcc
     7441 ctgccttcga gctctgcgtc aataccgcgc gcgagccggg cagcgaagag tatttggatt
     7501 ccgagaaata ccagattaaa gaatggaata tcgaatcggc cggcagtctg cgcgagttga
     7561 tggcgcggat taaccagatc cgcgcggcaa atccggcgct ccatcataat cgcagcttgc
     7621 ggttttatac cgtggacaat gacgagatta tctgtttcgc taagcatagc gaagatcgct
     7681 ctaatgctat cgtcgttgcg gttaatctcg atccgcatca cacccgatcc ggatgggtgg
     7741 atttgccgct ggcggaactg ggtctcgatc cggccaagcc ttttcagatg catgatctac
     7801 tgaccgatgc gcgctatctg tggaacgggc cgcgcaatta tttgagcctc aatccagcga
     7861 gcgtgccggc gcatatcttc cgagtgcggc ataacctgcg ccgggagcag gatttcgatt
     7921 acttcatgta gcaggctgct gaaaagactc ataggcttcg tttgcgcgac gactcgcttt
     7981 aacgtgctga agcgtacgcc tcagctcatc gagcttcacg cgcctcagct ctgaggtctt
     8041 tttgagcagc ctgcaaccga gtctttcagc aactgttagg aagtgaagca gcaacggtga
     8101 gccgcaaacc cgctaaatcc acgtcgactt ttctcgacga caacccgctc tggtatcagg
     8161 acgcggttat ttacgaggtg cacgttcgcg ccttccatga cggcaacggc gacggcatcg
     8221 gtgattttca ggggctgacc gaaaaactcg attatctcca ggacctgggc gtaaccgcgt
     8281 tatggttgct gccgttctat gtctcgccgc ttaaagacga cggttacgac atcgccgact
     8341 acacccagat ccatcccgac tatggaacgc tgcacgattt tcgcctcttt ttgcgcgggg
     8401 cgcacgaacg cgggctccga gtgatcaccg agctggtcat taatcatact tcggatcagc
     8461 atccgtggtt tcttcgtgcc cgggatgcca cgtctggcag cagcgcgcgc aacttttatg
     8521 tctggagcga cacgcaggag cgctatcccg aggcacggat tattttcaag gacttcgagc
     8581 cttctaactg gtcttgggat tcagtggcca aggcctacta ctggcatcgt ttttattcgc
     8641 accagccgga cctgaatttc gacaacccgg aagtgcacaa ggcgattttc gatgcgctcg
     8701 acttctggct cgatatgggc gtagacggcc tgcgcctgga tgccgtgcct tatctctatg
     8761 agcgcgaggg caccaactgc gaaaaccttg cggagaccca cgcatttctc aaaaagctgc
     8821 gccaacacgt cgacagcaag tacaagaacc ggatgttgtt ggccgaggcc aatcagtggc
     8881 ccgaagatgc agtggcgtat ttcggcaatg gcgatgaatg ccatatggcg tttcattttc
     8941 ccgtcatgcc gcgtcttttt atgtccgtat atatggagga tcgctttccg attatagaca
     9001 ttctcgatca gacgccggcc atacccgaat ccagtcaatg ggcgctgttc ttgcgcaatc
     9061 acgatgagtt gacccttgag atggtgaccg atgaagatcg cgactacatg tataaagtct
     9121 acgccagcga tccgcgggcg cggatcaact tgggaattcg gcgccgcctt gcgccgcttc
     9181 tcggcaataa tcgccgccgc gtagaattgc tcaacagcct gctgttctcc ctgcctggga
     9241 ccccggtgat ctattacggc gatgagatcg gcatgggcga taacatttac ctaggcgatc
     9301 gcaacggcgt gcgcactccc atgcagtgga gcgcagaccg caatgccggt ttctcgcgcg
     9361 ccaacccgca gcgcctttat ctgccggtga tcatcgatcc ggaacatcat tacgagaccg
     9421 tcaatgtcga ggcgcagcaa cagaacccgg ggtcgctgct ttggtggatg aagcggctgg
     9481 tgttgctgcg caaaaattat caagcctttg gacggggaag catcgagttt cttttgccgg
     9541 ataaccgtaa gatgctggtg ttcgtccgtc gtctcggcga tgagatcatt ttggtggtgg
     9601 ctaacctctc gcgtttcgtc aactacgtcg agctcgatct gtcggcctac aagggcatgg
     9661 ttccggtgga gctcttcggg cacacgcgct tcccctcgat tggtgaacta acctactttc
     9721 ttacattagg acctcatgca ttttattggt tcaagctcga gcagacgcgg gtttcggaag
     9781 cgcgagcggg agctgaggga tacgagttgc caagcttgga ggtggagggc agttggcagg
     9841 ctgttcttga gggtcgaccg cggactatgc tcgaacggag ccttcccgct tatcttttaa
     9901 cctgtcgatg gttcggcggc aaagcgcagc agatccggtc agtgaaaaat ctcgatgtca
     9961 tcccgttccc ggtggactcg gcaactattt acctgaccac ctgggcggtg gattacatcg
    10021 gcgggacgcc ggaaacctac ctgctgcctc tggcgtatgc cagcggtgat cgcgccttcg
    10081 agctgcgcca atcggcttcg caggcgatca tcgctcagct caagatcgaa gatgaagcag
    10141 gggaaagcga agggatcctg tacgacgccg tgcatgagcc aggttttctc aaatcgttgc
    10201 tgacggcgtt ggcccggggg cggcgattta aagataccgg ggccgagctg ctggcgcacc
    10261 cgtccaaagg atttcgcgaa ttgatgggta ccccggaaac cgcgcttgaa ccgctgctga
    10321 tcaaacgcga acagagcaac acttcagtag cctacggcga tcgactgatg tttaagcttt
    10381 ttcgccgcgt caccgagggg ctcaatcccg acgttgagat cggccgattt cttaccgaac
    10441 tggagtttgc ccatgtgcca gcgttggccg ggcacctcga gctgcgcaag ggacgaggcg
    10501 aacccgctac tgtcggcatt ctgcagcggt tggtagctaa tgaaggcgac gcctggcgct
    10561 atactcaaga cagcctgggt tattacttcg aacaagtttt aactcgccag gtcgagctcg
    10621 gcgaggccag tttaccgaat gcatcaccgg tagaactcac ccagcacgag actcccgagc
    10681 tagcca
//
LOCUS       DASUTG010000803         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1013918, whole genome shotgun sequence.
ACCESSION   DASUTG010000803 DASUTG010000000
VERSION     DASUTG010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013918"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VF089_18670"
     CDS             complement(<1..1307)
                     /locus_tag="VF089_18670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176041.1"
                     /translation="MPDGNKKTDRNTRENVSRRQFIKLAGTTAVALGTLPWPKAPLFA
                     AQPARKRIIIHGERQVTSLGYHNRRETEHVSMVDAGLVGQNPATLERVPVLAEELPSV
                     KKGTWKIDPQKKTMITVYKLRPNLKWHDGKPYTSKDFEFGWQIAKHPQFPMPDRLVPE
                     MISKIETPDDRTLVIHWNDLYNEAYAIQYTHVRAFPRHLLQEAFNAGDMKAFANLPYW
                     NKNFIGAGAYRVVEWDAGSRMELEAFNDFALGKPKIDRVTYKTVEDNNTNLAAVLAGE
                     VDLCMRSTISFDGAMILRDQWEKTGKGKVHISPASWTWLNLSRDNPFFSDVSVRRALL
                     HAIDRDAMVQNMFRGEKVVSDIPLSRVRKTYKKALSTATIYKYDPERAKKLLAEAGWK
                     PGSDGILTNAKGERMEFEFRVTAERRDHEQAQAIIADYWKKIGV"
     gene            1522..2229
                     /locus_tag="VF089_18675"
     CDS             1522..2229
                     /locus_tag="VF089_18675"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6176042.1"
                     /translation="MALDNFEDHCWKDVVTPEILKTYTPYHRETYIGQRPALLAIDLY
                     NLAYEGGPKEPHELVDTHKSSCGVYAYNAIKPTQELFALARSLKIPIFYTTTETRKEA
                     NPDKVYATNRQRVKIDPHAFEIREEFKPEADDVVIFKERASGFFGTPLIAHLTQKGID
                     SLIVCGESTSGCVRASSVDGYSLGYHVSIVEECVFDRSPLSHKVNLFDMHHKYCDVMK
                     LQEVKEKIKLERPRNAK"
     gene            2363..>3141
                     /locus_tag="VF089_18680"
     CDS             2363..>3141
                     /locus_tag="VF089_18680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176043.1"
                     /translation="MYSTANGLKIYYEVSGEGFPFVMVHANPFDHNLWMYQIAHFSTY
                     FKVIAIDIRGYGRSDKPATPFTLKDMADDVLGVCRDEDVREAILGGVSVGSGMALLLG
                     LDYPEMFKALILVGGSSGPGGAIDERIHGYTKVGIEKYHIQHLNELVAPDFPETKLGK
                     YLLNTYVERDPWLSGESIAQIFRARGGTDMTPRLGSMKVPTLVINGEYDNSLRGGQRT
                     ASLIPGAVHKILPKTGHACCIEDPAGFDEFVIDFLRANRLLP"
BASE COUNT          718 a          881 c          808 g          734 t
ORIGIN      
        1 acgccgatct tcttccagta atcggcaatg atcgcctgag cctgctcgtg atcgcgccgt
       61 tcggcagtga cccggaattc gaactccatc cgctcgcctt tagcattggt caaaataccg
      121 tcggagcccg gcttccaacc tgcctcggcg agaagttttt ttgcccgttc agggtcgtac
      181 ttatagatgg tcgccgtcga taacgctttc ttgtaagtct ttcggacccg cgatagcgga
      241 atatccgaga ccaccttctc tcccctaaac atgttctgca ccatcgcgtc gcggtcgatg
      301 gcatgcagca acgcacggcg cacactgaca tcactgaaga atggattgtc gcggctcagg
      361 ttgagccaag tccaggaggc aggactgata tgcactttgc cttttccagt cttttcccac
      421 tgatcacgca ggatcatcgc gccatcaaaa gagatagtgc tgcgcatgca caggtccact
      481 tctccggcca gcaccgccgc aaggttagta ttgttgtctt caaccgtttt gtaagtcacc
      541 cggtcaattt tgggcttgcc gagcgcgaag tcattaaagg cctcgagttc catgcggctg
      601 cccgcgtccc attcgaccac gcgataggcg ccagcgccga tgaagttttt gttccaataa
      661 ggcaaattgg caaaggcctt catgtcgccg gcgttgaagg cttcctgcaa caggtggcgt
      721 ggaaaggccc gcacgtgcgt gtactggatc gcataagcct cgttgtagag atcgttccaa
      781 tggatcacca gggtgcgatc gtcgggggtt tcgatctttg agatcatttc cggcaccagc
      841 cgatcgggca tgggaaattg gggatgtttc gcaatctgcc agccgaactc gaagtctttg
      901 gaagtatacg gcttgccgtc atgccatttg agattaggac ggagcttgta caccgtgatc
      961 atggtttttt tctgaggatc aatcttccac gtccccttct tgaccgaagg cagttcctcg
     1021 gctagcaccg gaacgcgttc gagagttgca gggttttgac cgaccaggcc cgcatcaacc
     1081 atcgacacat gctctgtctc gcgacgattg tgatagccga gactcgtcac ttgacgctcg
     1141 ccatgaatga tgattctttt tcgtgccggc tgagcggcaa aaagtggcgc tttcggccac
     1201 ggcagagtgc cgagcgccac cgcagttgta ccggccaatt tgatgaattg ccgccggctg
     1261 acattctcgc gggtattcct atctgttttc ttgttcccgt ctggcatcga gtcctccatt
     1321 ttagcgctac gatcgtcggc atccatataa ctaacctcga tcttgagtgt caatcgatgt
     1381 ggcaagacgg atgactattc gtcgtccgcg ccgccgatca ccgcctctta tttccagaat
     1441 gtccttgtct ctgggtcttc cttgacaagg cttcaagcca ggggatacaa acaaacaatc
     1501 tcatcgagta aggagaaacc catggccctg gacaattttg aggatcattg ttggaaagac
     1561 gttgtaactc cggaaattct taagacctac acgccgtatc atcgcgagac ctatatcggc
     1621 cagagaccgg cgctgctggc catcgacctt tacaaccttg cctatgaagg cggccccaaa
     1681 gaacctcatg agttggtcga tacccataag agctcgtgcg gcgtttacgc ttacaacgcg
     1741 attaagccga cccaggaact tttcgcactg gcgcgttcgc tgaaaatccc gatcttctac
     1801 accacgacgg aaactcgcaa ggaagccaat cctgataagg tttatgcgac caaccgtcaa
     1861 cgggttaaga tcgatccaca cgcttttgaa atccgcgaag agttcaaacc cgaagccgat
     1921 gatgtggtca tcttcaaaga gcgcgccagc ggcttcttcg gcacgccgct aatcgctcat
     1981 ctgacacaga aagggatcga cagcctgatt gtctgcggcg aaagcaccag cggctgtgtc
     2041 cgggcgagct cagtcgatgg ctattcgctg ggatatcacg tgtcgatcgt ggaagaatgc
     2101 gtcttcgatc gaagcccgct gtcacacaag gtcaatctct tcgatatgca tcacaaatac
     2161 tgcgatgtga tgaagctcca ggaagtgaaa gaaaagatca aactggagcg gccgcgcaac
     2221 gcgaagtaga actgttcaaa acgttcaagt cgttcaactc gttcaaaagc gtcagagaag
     2281 gtgaaacaga acgtgttctg acggcttgaa cggaacgcag caattgaacg gcttgaacgt
     2341 tttgaacgca agtagtcatt cgatgtacag caccgccaac ggtctcaaaa tctactacga
     2401 agtctccggt gagggatttc cctttgttat ggtccatgcc aatccattcg atcataactt
     2461 atggatgtat cagatcgcgc acttctcaac ctatttcaaa gtaatcgcta tcgatatccg
     2521 cggctacggt cgttcggata aaccagccac tccatttaca ctcaaagaca tggccgacga
     2581 tgtccttggg gtctgccgcg atgaggacgt cagggaagcc attctcggcg gcgtgagtgt
     2641 cggctcggga atggcgcttc tgctcggctt ggactatccg gaaatgttca aggctctgat
     2701 cctcgtcggc ggtagcagtg gtccgggcgg cgccatcgat gagcgcattc acggttatac
     2761 caaagtcggc atcgagaaat accacattca acacctaaac gaactggtcg cgccggattt
     2821 tcccgaaacc aaactcggca aatatctgct caacacctat gtcgagagag atccatggct
     2881 ttccggggaa tccatcgccc agatttttcg cgcccgtggc ggcaccgaca tgacgccgcg
     2941 gcttggatcc atgaaggtgc cgaccttagt gatcaacggc gaatacgaca actcgctcag
     3001 aggcggccag cgcaccgcct cgctgattcc gggcgccgta cataaaatct tgcccaagac
     3061 cggccacgcc tgctgcatcg aggatccagc cggcttcgat gagttcgtca tcgatttcct
     3121 gcgcgccaat cgtctcctgc c
//
LOCUS       DASUTG010000804         4406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1604413, whole genome shotgun sequence.
ACCESSION   DASUTG010000804 DASUTG010000000
VERSION     DASUTG010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604413"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..813)
                     /locus_tag="VF089_18685"
     CDS             complement(37..813)
                     /locus_tag="VF089_18685"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176044.1"
                     /translation="MTSFIRAQEVSLVFKAKDREPVTALSNFSLDIAQGEFVSIVGPS
                     GCGKSTFLNIVLGLIEPASGVVQLNGTRIRGPGQERAMVFQEFGLLPWRSVTANVELG
                     LELKGIPAAKRAARAKELINLVGLKGFENHYPHELSGGMKQRVGLARALATEPEVLLM
                     DEPFAALDAQTRDLMQSELLQIWERSKKTVLFVTHSIEEAAYLSDRVIVMTARPGRTK
                     DVIQIDLPRPRDYEMRLTPEFNEIKLRIWEVLREELSSRG"
     gene            1105..1527
                     /locus_tag="VF089_18690"
     CDS             1105..1527
                     /locus_tag="VF089_18690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176045.1"
                     /translation="MKVKQIYHIGLPCNDLERATKFYTDVLGMKCTKVGFDTESGGPY
                     KDVYGMFPEISRLFMEDGMCLVLFQRPKPIERGDFDEGTSHIALEVSSEDFDKAADEL
                     KKAGVKVLIDKPVVRPTGKAIYFFDPEMNYIQLWSKPQ"
     gene            complement(1570..2391)
                     /locus_tag="VF089_18695"
     CDS             complement(1570..2391)
                     /locus_tag="VF089_18695"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6176046.1"
                     /translation="MNSNYPRGPLREGEPIILLDRKDREYLTRLDRNRPLAIRGGKVP
                     VDQLIGRDEGCTVRSSVNEPFLVFRPSLPQLIPNLPRNAQVVYPKDLGPILIWADLFP
                     GARVVEAGVGAGALSMTLLRAIGSSGQLITYELREDFADMARRTVSKYFGPTPNWTLK
                     VGDVETELSEADVDRVVLDLPEPWKVIDRAWSALKPGGILLSYLPTVIQVKSLVDALR
                     ADKRFACIETMETLMRFWHIKGMSVRPQHRMIAHTGFLTTARRLADGEKDIPRAS"
     gene            complement(2402..3526)
                     /locus_tag="VF089_18700"
     CDS             complement(2402..3526)
                     /locus_tag="VF089_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX6176047.1"
                     /translation="MRKRLYSVIIAGGRGTRFWPLSRSRKPKQLLKLFGAKTLLDETA
                     DRVLSLGGSDQTLVVTVAEQLNAVRKELSGLPRKNFLAEPEGKNTAPCIGLAALEVFA
                     RDPDAVMIILPADHWVSDVPAFRRTLKTAAELAAWHDRLVTIGIQPAYPETGYGYIIK
                     GKPLAGTADAYQVKRFKEKPDVKIARQLLRQGSLWNAGIFAWRASTLLALMERYQPAI
                     HNCLVKIADAARGKSLASPTPKLSSVIAREYKEMPSISIDHALLEPAGSDGRVLTVPG
                     QFGWSDVGSWAAVHQMSARDANGNASNGMWLGLGSKDCLIHAPDRLVVLLGMRDTFVV
                     DTPDALLVGDLKRSQEVRDLVDELSKRGYRSYTIDESTSG"
     gene            3542..>4406
                     /locus_tag="VF089_18705"
     CDS             3542..>4406
                     /locus_tag="VF089_18705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6176048.1"
                     /translation="MKVRPHTTVGEVAVQGSSRNLTWEIGAAEEGGRLESFVRRHLPH
                     LSRRQSTKAIDESAFLVNGRRATKGQRLKQGDRVVFHGAKSWLSELPVAAVRGRVTVI
                     YEDTEIIAVDKPAGIPTHGFSGRDVNTLANMIVARWPELSSVGRSRWEPGLLNRLDTE
                     TSGLVLVAKNQPAFNNLREQLQRRRVTKKYIALVWGEVASSGSISLALAHDLTDRRRM
                     QVVDDFGSNRRHLRSWHALTSFRKLASSAEMSLVEITMETGVTHQIRVHLAATGHAIV
                     GDTSYGFVSYST"
BASE COUNT          971 a         1165 c         1231 g         1039 t
ORIGIN      
        1 gccggtgttc cgtaaaaaat gcttgtgcag ccatttctat cctcgggagc tcagctcttc
       61 tctcaagact tcccagatgc gaagcttgat ctcattaaat tccggtgtaa gccgcatctc
      121 ataatctctt ggtcgtggca aatcgatttg aatgacgtct ttggtccggc ccggccttgc
      181 cgtcatgacg atgactcggt ctgaaagata ggcagcctct tcgatgctgt gggtgacaaa
      241 gagcacggtt tttttggagc gttcccaaat ctgcaataat tccgattgca tcaagtcgcg
      301 cgtctgtgca tcgagtgcgg caaagggctc atccatgagc agtacttccg gctcggttgc
      361 cagcgcgcga gcgaggccga cgcgctgttt catacccccg gagagctcat gcggataatg
      421 gttttcaaag cctttcaatc ctaccaaatt gatcagttcc tttgctcgtg ctgctctttt
      481 tgcagccgga attcccttca attcaagtcc gagttctacg ttggccgtga cgcttcgcca
      541 cgggagtagc ccgaactctt gaaaaaccat cgcccgctcc tggcccgggc ctctaatgcg
      601 cgtgccgttc aactgaacta cgccgctggc tggttcaatc agccccaaga cgatgttaag
      661 gaaggtactt tttccgcacc ccgacggtcc gacgatactg acaaactcgc cttgggcaat
      721 gtcgaggctg aagttactga gcgccgtgac cggctctcga tccttggctt tgaagactag
      781 agagacctcc tgagctctaa tgaaagatgt catgggaacc ttacgttcac tgataggcca
      841 ttgcgcagct acagcgtggc tgatgatgca gcgttactcc aagcagaacc gtttgtaata
      901 tccctgtcga acccatctat agatttctat ctcgttgatg tctagttatt ccgctcctcg
      961 gtggttttct gtgggcattt agacttgccg atgatgatct gtcaagaatg tcgtcactga
     1021 aagataggct tttccagaac ctgaagctat tggaattccc tcgcgcgttg cagtatagac
     1081 aggatgaaga caaggaggtg actcatgaaa gtaaaacaga tctaccatat cggcttgccg
     1141 tgtaatgatc tggagcgcgc aaccaagttc tacacagatg tactcggaat gaagtgcacc
     1201 aaggttggtt tcgacaccga aagtggcggt ccttacaaag acgtctacgg tatgtttccc
     1261 gagatctccc ggctgttcat ggaagacggc atgtgtttag ttttgtttca gcggccaaag
     1321 ccaattgaac ggggtgattt cgacgagggg acttcgcata ttgcgttgga agtcagctcg
     1381 gaagatttcg ataaggcggc cgatgagctc aagaaggcgg gcgtgaaggt gctgatcgac
     1441 aaaccggtcg tgcggcctac cggtaaagcg atctacttct tcgaccctga aatgaactac
     1501 attcagcttt ggtcaaagcc tcagtgagga ccgactccag ggaatgtcgt ggtacccccc
     1561 agcgctgatt caggaagctc ggggaatgtc tttttcgcca tctgctaagc ggcgcgcggt
     1621 agtcaagaaa cccgtatggg cgatcattcg gtgttgcggc cgaacgctca tgcccttgat
     1681 gtgccaaaag cgcatcagtg tttccatcgt ttcgatacag gcaaaacgtt tgtcggcgcg
     1741 cagggcatca accaagctct tcacctggat cacggtcggt agatagctga gcaggatgcc
     1801 accgggtttt agggcgctcc aggcgcggtc gatcaccttc cagggttcgg gtaaatccag
     1861 aacgacgcga tcgacgtcag cttccgagag ttcggtttcg acatcgccga ccttcaatgt
     1921 ccagttcggc gttgggccga agtatttgct aacggttctt cgcgccatat cggcaaaatc
     1981 ttcgcggagt tcataggtga tgagttgacc cgagctgccg atggcgcgta gcaacgtcat
     2041 acttagagcc ccggcgccga cacctgcttc gaccacacgc gcccccggga agaggtcggc
     2101 ccaaatgaga atcggcccaa gatccttagg ataaacgacc tgcgcgttgc gcggcaaatt
     2161 gggaattaac tggggcaaac ttggtcgaaa aaccaggaac ggctcgttga cagaagaacg
     2221 cacggtgcaa ccttcgtcgc gaccgatcag ttgatcgacg ggcactttgc cgccacggat
     2281 cgccagcggc cggtttcggt cgagccgagt gaggtattct cgatctttgc ggtcgagcag
     2341 aatgatcggt tcgccttcgc gtaatgggcc cctgggatag ttggaattca caagattata
     2401 atcagcctga tgtggattcg tcgatggtat aagagcggta gcctctcttg ctcagttcat
     2461 cgactaagtc gcgaacctct tgtgagcgct tgaggtctcc gacaagtaac gcatccggcg
     2521 tatcgacgac gaaggtatct cgcatcccca gtagaacgac tagacgatcg ggggcatgaa
     2581 tcaggcagtc cttcgagccc agcccgagcc acatcccatt actagcgttg ccgttggcat
     2641 cacgcgccga catttggtgc accgccgccc aactaccgac atcgctccag ccaaattggc
     2701 ccggcacggt caagactctg ccgtcggaac cggccggctc gaggagcgca tgatcgatgg
     2761 aaatgctcgg catttctttg tattcgcggg ctatcacaga gctaagtttt ggagttgggc
     2821 ttgccagcga tttgcccctg gcggcatcgg caatcttcac gagacagttg tgaattgccg
     2881 gctggtagcg ctccatcagc gccagcagtg tagatgctct ccaggcgaag atgccggcat
     2941 tccacagaga accttgacga agtagttggc gcgcgatttt cacgtccggc ttttctttga
     3001 accgtttcac ctggtaagca tctgcggttc cggctagtgg cttgcctttg ataatgtagc
     3061 catagccggt ttccggatag gcaggttgga taccgatagt gaccaggcga tcgtgccagg
     3121 cggcgagttc agcagcagtc ttgagcgtgc ggcggaaagc cggcacatcc gacacccagt
     3181 gatctgcggg tagaataatc attaccgcgt cgggatctct ggcgaacact tccaatgctg
     3241 ctaggccgat gcacggcgca gtgttctttc cttcaggttc ggcgagaaag ttcttccgtg
     3301 gcaagccact caactcttta cgcaccgcat tcaactgttc ggctaccgta acgaccagcg
     3361 tctgatcgct tcccccgagt gaaagtactc gatccgccgt ttcgtcgagc agagtcttag
     3421 cgccgaagag cttaagtagt tgtttgggct tgcgcgaacg gctgagcggc cagaaacgtg
     3481 ttcccctgcc gccggcgatg atgacactgt aaagtctttt cctcatgcta gaatgttatc
     3541 catgaaagtg cggcctcata ctacggtagg tgaagtggcg gttcaaggat cgagccggaa
     3601 tctcacctgg gaaatcggcg cggccgaaga gggaggccgc ctggaaagtt ttgtgcgccg
     3661 ccacctgccc catttatcgc gtcgccaatc cacgaaagcc atcgatgaga gcgcgttttt
     3721 ggtaaacggt cggcgcgcaa ccaaggggca aagattaaag caaggggatc gagtggtctt
     3781 tcacggggcg aagagttggc tttctgagtt gccagtggca gcggtaagag ggcgtgtcac
     3841 cgtcatttat gaagatactg aaatcatcgc ggttgataaa cctgccggca ttccaactca
     3901 tggattttcc gggcgcgacg tcaacaccct ggccaatatg atcgttgcgc gatggcctga
     3961 gctatcttct gtagggagga gccggtggga gccgggtttg ctgaaccgat tggacaccga
     4021 aacttccgga ttagtgctgg tggcgaaaaa tcagccggct tttaacaacc tgcgggagca
     4081 gttacaacgt cgcagagtca ccaaaaaata catcgcgctg gtgtgggggg aggtcgcttc
     4141 ttccggatcc atcagcttgg cacttgctca tgatttgact gatcgccgcc gcatgcaggt
     4201 ggttgatgat ttcggatcga atcggcgcca cctaagaagc tggcatgcgt taacctcttt
     4261 tcgaaagctc gccagttctg cggaaatgag ccttgtcgaa attacaatgg aaaccggcgt
     4321 cacgcatcag attcgggtcc atctcgccgc tactggtcat gccatagtgg gcgatacgag
     4381 ctatgggttt gtaagctatt cgacca
//
LOCUS       DASUTG010000805         6134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_124839, whole genome shotgun sequence.
ACCESSION   DASUTG010000805 DASUTG010000000
VERSION     DASUTG010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124839"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VF089_18710"
     CDS             <1..805
                     /locus_tag="VF089_18710"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6176049.1"
                     /translation="FAYTPFPDESEHLWRGYLDPSVSNYRKEVADRLRPFLEQVYKSS
                     DDHLGLFLANRPADTIIAVISDHGMQAADKRFAINRLLQQQGLLVIDEQGRIDLSRTK
                     VLYPSINNGYLLINSIDRKNGIVALDQRKEVARHVTEALLEVRDGDQPVVKNVYDADI
                     RGAEMGIGGPSGGDIYVELVPGYDFDPRTTPDPLITQVEPYGTHGGDPEQSSMRTLML
                     FHGPGIAAGRRLQNVRITDFAPTLSALLDFPAPRNSSGRVLRDVSAALH"
     gene            873..2165
                     /locus_tag="VF089_18715"
     CDS             873..2165
                     /locus_tag="VF089_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176050.1"
                     /translation="MSSRSTWEPAPVVSREEVLELHHEVAAMPDISLNIREDIFRIDV
                     LEMAWDIGVVVYEPADATRVPSGPDGNRIGVFLLHGGVSDYKSVDGIARILAQKFAIK
                     VASMTFPGRFYLLDPSRDWPGDVESSDGSARTPLWSKETNISRDQYDIVQDGSKRESY
                     GTLISLSAKQGTEFYHRMAAWPVAFEEAIRETARRHLPQGEFSIYIHGHSTGGPFAMM
                     ATQRLANIVGVIGYGTSPFGYMYPQVSGDNWEFPFNQLRLRTWRDTARYLYEGMKDKG
                     LGLPMLIELTFERWEKSKKRPNFKAEDFVHKNSLKALESAARAAAERLKMSAKDSDAL
                     VQRYLGYTREVSGPGVKPVPPFLSLHGINDDTVTLARCDKSLSLFAKLNPAPKVRCVS
                     IGAGVHTWSHTEPELPQGIATPVAKLWHDAIMNGFFFR"
     gene            2182..2733
                     /locus_tag="VF089_18720"
     CDS             2182..2733
                     /locus_tag="VF089_18720"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6176051.1"
                     /translation="MAEKDHLILTAVGPDQVGLVEKLSEFISSHGCNIDDSKMAVFCG
                     EFAVILLITGEPGSLFKIANDYRVLEAQTSLTISIKTPSKRKSPESYLPFRLIASCMD
                     HPGIVYQLSSVLSALGINIESMETKTYAAPDSGTPIFRLEANISVPAKVNINALRERF
                     FEIQREENIDVELSPIHPSPRIS"
     gene            3093..5732
                     /locus_tag="VF089_18725"
     CDS             3093..5732
                     /locus_tag="VF089_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6176052.1"
                     /translation="MIKEEQRLEEDRRRKANWKRWGPYLADRQWGTVREDYSPYGDAW
                     EYLPHDHARSRAYRWGEDGIGGISDRHQYICFALALWNGKDPILKERLFGLTGNEGNH
                     GEDVKEYYFHLDSTPTHSYMKFLYKYPQAEFPYARLVEENRRRSKREMEFELIDTGIF
                     DDNRYFDVFIEYAKAAPDNILIRIEAINRGPEAAPLHLLPTLWFRNTWAWGLDPRRPR
                     LRRDAAAEMAAINLNHYYYGKLWLYCEKNPELLFTENETNYSRLFDAENAARFVKDGI
                     NDYFIHKAMHAVNPECSGTKAAAHYSALLQPGASLTARLRFSNLSPTASESPDGKFDQ
                     IVAQRRHEADEFYRSIIPQNTSQDRRNIMRQALAGLLWSKQVYHYDVERWLKGDPSGP
                     EPSRERLKGRNSDWRHLYNADVISMPDKWEYPWYAAWDLAFHCVALALVDADFAKQQL
                     LLMTREWYMHPNGQLPAYEWAFGDVNPPVHAWAAWRVFKVEKKRRGIGDRQFLERIFH
                     KLLLNFTWWVNRKDAAGRNVFQGGFLGLDNIGVFDRSAPLPTGGHIEQSDATSWMGMY
                     CLNMLAIALELARENPVYEDVASKFFEHFVYICEAMHNLGGAGMELWNAKDGFYYDVL
                     NMTNGERLPMKVRSMVGLIPLYAVETLDPVIVDALPGFKRRMQWFIENRPRLSEHIET
                     LSTDEGPQRFLSLVNRHRLKRVLQYMLDENEFLSAYGIRALSRYHRDHPYTLKVDGLE
                     YRVDYEPAESSTGLFGGNSNWRGPIWFPVNFLIIESLQKFHFFLGDSYKVECPTGSGR
                     TANLWQVAGEISQRLTRIFTRDQNGRRPVFGATEKFQSDPYWRDLIPFNEYFHGDNGA
                     GIGASHQTGWTALVAKLIQQYGE"
BASE COUNT         1415 a         1732 c         1666 g         1321 t
ORIGIN      
        1 ctttgcctac accccgtttc cggatgagag cgagcacctc tggcgcggct atctggatcc
       61 cagtgtctcg aattaccgga aagaagtcgc agatcggcta cgaccgttct tggagcaggt
      121 ctataaaagc tccgatgatc atttaggtct tttcttagcc aatcgtccgg cggacacgat
      181 aatcgctgtc atctccgatc atggcatgca agcggctgat aaacgttttg cgatcaatcg
      241 tctgctacaa cagcaagggc tgctggtaat cgatgaacaa ggtcgaatcg atctgtctag
      301 aacgaaagtc ctctatcctt cgatcaacaa tggatatctg ctgatcaact ccatcgatcg
      361 caagaacggt atcgttgctc tcgaccaacg taaagaggtc gcgcgccacg taactgaagc
      421 gctcttggag gtccgcgatg gcgaccaacc ggtggtgaaa aacgtttatg atgctgatat
      481 ccgcggcgca gaaatgggca tcggcggtcc ctcgggcggg gacatctacg tcgagctcgt
      541 tcccggctat gatttcgatc cgcggacgac tccggacccg ctgatcacgc aagtcgaacc
      601 ctacggcacc catggaggcg acccggagca aagttcgatg cgcactctca tgctcttcca
      661 cggcccggga atcgccgcgg gacgccggct gcaaaacgtt cgcattaccg atttcgctcc
      721 aacgctttcc gcgctactcg attttcctgc tccacgaaac tcaagcggcc gcgtgctgcg
      781 cgacgtttct gccgctctgc attgacacag ttgttggctt tcgcatagtc ttaaattcgt
      841 caaactcacc agtcccagaa gaacatccga cgatgtcgag ccgatcaact tgggaaccag
      901 cgccggtcgt ctcccgtgaa gaagtcttgg agcttcatca cgaggtcgcg gcgatgccgg
      961 atatctcgtt aaatattcgc gaggacattt tccgcattga tgtcttggaa atggcctggg
     1021 atatcggcgt cgtggtttac gaacccgccg atgccacgcg agttccttcc gggcccgacg
     1081 gcaatcgtat cggcgtcttc ctcctgcacg gcggcgtctc cgattacaaa tccgtcgacg
     1141 gcatcgctcg aatactggcg cagaagttcg ccatcaaagt cgccagcatg acttttcccg
     1201 gcaggttcta cctgctcgat ccaagcaggg actggcccgg cgatgtcgag tcctccgatg
     1261 gctcggcacg caccccgctg tggagtaaag aaaccaacat aagcagggat cagtacgaca
     1321 ttgtgcaaga tggttccaag cgtgaaagct acgggacttt gatttcgctc tcggccaaac
     1381 aaggcactga attttaccac cgtatggcag cctggccggt ggcgttcgag gaagccatca
     1441 gagaaaccgc caggcgccat ttaccgcaag gcgagttttc catctatatc cacggccact
     1501 ccaccggagg gcccttcgcc atgatggcaa cgcaacgtct cgccaatatc gtcggcgtca
     1561 tcggttacgg cacttctcca ttcggttaca tgtatccgca agtcagcggc gataactggg
     1621 agttcccgtt taaccagctg cgactacgca cctggcgcga tacagcccga tacctctacg
     1681 aaggaatgaa agacaaaggt ctgggcttgc cgatgctgat cgagctcacc ttcgagcgct
     1741 gggagaaaag caagaaacgg ccgaacttca aagctgaaga cttcgtgcac aaaaattctc
     1801 tcaaggcgtt ggagagcgcc gctcgtgctg cggcagaacg gttgaaaatg tcggcaaagg
     1861 acagtgatgc gctggtgcaa cgctatttgg gctacacccg agaagtatcc ggtcccggag
     1921 tcaaacctgt gccgcctttc ctgtcgcttc atgggattaa cgacgatacc gtcaccttgg
     1981 cgcgctgtga caagtcgctg tcgctttttg ccaaactgaa tcccgctccc aaagtccgct
     2041 gcgtctccat cggcgcggga gtacatacct ggtctcacac ggagcccgag ctgccacagg
     2101 gcattgccac gccggtggcg aaactctggc acgatgcaat catgaacgga tttttcttta
     2161 ggtgatttac gaagagcatc tatggctgaa aaagatcatc ttattctaac agcggtggga
     2221 cccgatcagg tgggtctggt cgaaaaactt tcggaattca tctcgagcca cggctgcaac
     2281 atcgacgata gcaagatggc agttttctgc ggcgaatttg ctgtaatcct cttgatcacg
     2341 ggagagcccg gcagcctttt caagattgcc aatgattacc gagtcctcga agcgcagacc
     2401 tctctgacga tctcgatcaa aacaccgtcc aagcgaaaga gcccggaatc ttacctcccc
     2461 tttcgcctga tcgcctcgtg catggatcat ccaggcatcg tttatcagtt gagcagcgtg
     2521 ttgagcgcgc tgggcatcaa catcgagtcg atggagacca agacgtatgc agccccggat
     2581 agcggcacgc cgatcttccg attggaagca aacatttcag tgccggccaa ggtgaatatc
     2641 aatgcgttgc gtgaacgttt cttcgaaata cagagggaag aaaacatcga tgtcgagctc
     2701 tcgccgatcc atccctcgcc aaggattagc taatttaccg cagtcgcagc aatcaagcct
     2761 tttcgcgcaa ccagcaaaca cgcccaagtt agtcgattgg cgaatagccc tgacacccta
     2821 tatcgcctgc cataggctta aaatattgca cagggctcca gctcccgcga tagcggcact
     2881 tggccgccac agcgaggcga agagcttgtt gggtgcacgg caaggagcca ctgccgcacc
     2941 aacccgcttc gaagtagcat ctaacgaatg gaagtagccg gtacaataca gtagaaatca
     3001 ccgagtcagg catcgcgggt tgacgtgcac tgtggaactc agagttctgc aacgtttaat
     3061 ctagtatccg gcaacctgct caggaacgtg aagtgatcaa ggaagaacaa agacttgaag
     3121 aggatcgccg gcgcaaggcg aactggaaac gttggggacc ctatctagcc gaccgccagt
     3181 ggggcacggt gcgcgaggac tacagtcctt acggcgacgc ctgggagtac ctgcctcacg
     3241 atcacgcgcg cagccgcgcc taccgatggg gcgaagacgg catcggcggc atctctgatc
     3301 gccatcagta tatctgcttt gctttggcgc tctggaatgg caaggatcct atccttaagg
     3361 aacggctctt cgggctcacc ggcaacgaag gcaaccacgg agaagacgtt aaggaatatt
     3421 attttcatct cgactccacg cccacccact cgtacatgaa gtttctctac aagtaccctc
     3481 aggccgagtt tccctatgcg cgactcgtag aggagaaccg caggcgctca aagcgagaga
     3541 tggaattcga gctgatcgat accggaatct tcgacgacaa tcgctacttc gacgtgttca
     3601 tcgaatatgc caaggcggcg cctgataata ttttaattcg catcgaggcg atcaaccgcg
     3661 gaccggaggc tgcgccgctg catctacttc ccaccctttg gtttcgcaac acctgggcct
     3721 ggggcctcga tccgcgcagg ccgcgcctgc gccgcgatgc ggccgcggaa atggcggcta
     3781 ttaacctcaa tcactattat tacggcaaac tctggctcta ctgtgagaaa aatcccgaat
     3841 tgctgtttac cgagaatgaa accaattaca gccgcttgtt cgatgctgag aatgccgctc
     3901 gcttcgtaaa agacggtatc aacgactatt ttattcacaa agctatgcac gctgtgaatc
     3961 ccgaatgttc gggaaccaaa gcggcagcgc attactcagc gctattgcag ccgggcgcaa
     4021 gcttgacggc tcggctccgt ttcagcaatc tttcgccgac ggcgagtgag tcgcccgatg
     4081 gcaagttcga tcagattgtg gcgcaacgcc gccatgaagc ggacgaattc tatcgatcga
     4141 tcattccaca gaacaccagc caggaccggc gcaatatcat gcgtcaggcc ttggccggtc
     4201 tgctctggag caagcaagtt tatcactatg acgtcgagcg ctggttaaaa ggcgatcctt
     4261 ccggacccga gccgtcgcgc gaacgtctca agggccggaa ttcggactgg cggcatcttt
     4321 ataatgccga cgtgatctcg atgccggaca aatgggaata cccgtggtat gccgcctggg
     4381 atctcgcctt tcattgcgtt gcgctggcct tagtggatgc ggatttcgcc aagcagcaac
     4441 tgctgcttat gacgcgggag tggtacatgc atccaaacgg tcaattaccg gcatacgagt
     4501 gggccttcgg cgacgtcaac ccgccggtgc acgcgtgggc ggcgtggcgg gtgttcaagg
     4561 tggaaaaaaa gcgccgcggc atcggcgatc ggcaattcct cgagcgaatt tttcacaagc
     4621 tgctgttgaa tttcacctgg tgggttaacc gcaaagatgc cgcgggcaga aacgttttcc
     4681 agggcggctt tttagggctc gacaatatcg gcgtattcga tcgcagcgcg ccgctgccga
     4741 ccggcgggca tatcgagcaa tcggacgcga ccagctggat gggcatgtat tgcctgaaca
     4801 tgctggcgat tgcgctggaa ctggcgcggg agaatccagt ttatgaagac gtggcgagca
     4861 agttcttcga gcatttcgtc tatatttgcg aggcgatgca caatctcggc ggcgccggta
     4921 tggaactttg gaacgccaag gatggattct attacgacgt cttgaacatg accaacggcg
     4981 agcgtctgcc gatgaaggtc cgatcgatgg tcgggttgat cccgctctat gcggtcgaaa
     5041 ctttggatcc ggtgatcgtc gatgctctgc cgggctttaa gaggcgcatg caatggttca
     5101 tcgagaatcg cccccgtttg agcgaacaca tcgaaacgct atcgaccgac gaaggtccgc
     5161 aacggttctt gtcgctggtt aaccgtcatc gcctaaagcg cgtgctgcaa tacatgctcg
     5221 acgagaatga gttcctgtct gcctatggca tccgcgccct gtcgcgctac caccgcgacc
     5281 atccgtatac gctcaaagtg gatggcctgg agtatcgggt cgattacgag cccgccgaat
     5341 cgagcaccgg tctttttggt ggcaactcca actggcgcgg gccgatctgg tttccagtaa
     5401 atttcctgat tatcgaatcg ctgcagaaat ttcatttttt tctcggcgat agctacaagg
     5461 tagagtgccc caccggctcc ggtcgaacag ccaatctctg gcaggtcgca ggcgagattt
     5521 cgcaacgcct aacccgtatt ttcacgcgcg atcaaaacgg gcggcggccg gtcttcggcg
     5581 ccaccgagaa gtttcaaagc gatccttact ggcgcgactt gatccctttc aacgagtatt
     5641 tccacggcga taacggcgcg ggcatcggcg ccagccatca gaccggctgg accgccctgg
     5701 tggcaaagct gattcaacaa tatggcgagt aggctgagaa ccggatccta agcgattcgt
     5761 gcggcgattc gagcatcgaa gtcatccgcg ctaaccgtca atgaactacc gcgcatcagt
     5821 gcgaggggcc gcagcacgta ggataagaat ttactgccaa aatgtcgccc ccgaatgtct
     5881 taatcggggg tccagttccg gtctcgcctg gattcccgct aaaagcatgc gggaatgacg
     5941 gattcgcaca ggtctcggaa taactcctca tgcagcaagc ttcgggaaac caaagccgcc
     6001 ggatcaactc gctacttatc tcaatacatc gatccggctg agaaacacga tcatctgctc
     6061 gatgatgaaa atatctaaat cgttagactt gtcgggctag attgggcaat attctcaacg
     6121 gccggatcga ttcc
//
LOCUS       DASUTG010000806         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_717196, whole genome shotgun sequence.
ACCESSION   DASUTG010000806 DASUTG010000000
VERSION     DASUTG010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717196"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VF089_18730"
     CDS             complement(<1..867)
                     /locus_tag="VF089_18730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176053.1"
                     /translation="MPSMMVATLGIALPEIRQSFSLSEVEAASLFSVLMIVAAITSTV
                     AGRFADRYGRKTILISGLSLLAAGFALAGITSSRLLFFLSLGITGLGYGFTPPALYAV
                     MSDLLPNKRGLGASLVSVAYGIGGAIGAVLASRIIAFSGWRAAFVTVGVIAAAIMLLQ
                     LYWIRNIRSIKSAVRGGSFKASLSLPLFILALAEFIGGSVFWSSAAWSPTLLRSDKAL
                     TLEETGWVMGILSLANMLGSSCLGALSDKLGRRRVIVLSSFPAALAAFVVFFWLESLL
                     GLALGMLVFGILK"
     gene            complement(952..1290)
                     /locus_tag="VF089_18735"
     CDS             complement(952..1290)
                     /locus_tag="VF089_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176054.1"
                     /translation="MRIYTGADNESHFEEKEIDLKLNGDMEVSELEPATGVFFRQAPT
                     HHLNDFHPAPRRQYIITLSGEVEIEVGDGTVKRFGKGDIMLADDTTGRGHITRVVGNQ
                     PRAYVTIPLK"
     gene            1427..>2282
                     /locus_tag="VF089_18740"
     CDS             1427..>2282
                     /locus_tag="VF089_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6176055.1"
                     /translation="MPELPDITVYIEALQDRLLNQTLQKIRIASPFILRSFDPPIQTA
                     EGKKVIGFDRLGKRIVFELEDELFMIFHLMIAGRFHWKKAGAPVPRKFGHAAFDFPPG
                     TLLLTEMGTKKRASLHLVRGREHLAEHDPGGLEIFGADLKAFRAALTDENHTLKRTMT
                     DPHVFSGIGNAYSDEILHRAGLSPIKQTQHLSEVEIERLYRATKEVLREWIKRLRTDT
                     GKNFPERVTAFRDDMAVHGKYRKPCPVCGSPVQRIAYAENETNYCAKCQTGGRLLADR
                     SLSRLLKAD"
BASE COUNT          572 a          625 c          649 g          436 t
ORIGIN      
        1 cttgaggatg ccgaacacaa gcataccgag ggcgagtccg aggagcgact ccaaccaaaa
       61 gaagaccaca aacgcggcca gggccgccgg gaaagaactc aacacgatta cccggcgccg
      121 gcccaacttg tccgatagag cgcccaagca ggacgagccg agcatgtttg ccaggctcag
      181 tatgcccatg acccagccgg tttcttcgag cgtgagcgct ttgtcgctac gcagcaaggt
      241 tgggctccag gccgccgagc tccagaaaac cgagccgcca atgaactcgg ccaaggcgag
      301 aataaaaagc ggcagcgaga gagaggcttt gaaagatccg ccgcggaccg cggatttgat
      361 cgagcggatg tttcggatcc aatatagctg cagcaacatg atcgctgcgg cgatcactcc
      421 gacggtgaca aaggcggcgc gccagccaga gaaggcgatg atccggctcg ccaagacagc
      481 accgatggcg cctccgatgc cgtaagcgac cgaaaccagg ctagcgccga gaccgcgttt
      541 gtttggtagc agatccgaca tcacggcgta gagcgccggc ggcgtaaagc cgtagcccaa
      601 tccggtgatt ccgagagaca agaaaaacaa gaggcgacta gaagtaatac cggccaaggc
      661 aaaaccggcg gccaaaaggc tcaagcctga gattaatatc gtcttgcggc cgtacctgtc
      721 agcaaaacga ccggcaacag tgctggtgat ggccgcgaca atcatgagaa ccgagaacag
      781 gcttgcggct tcgacctcag agagcgagaa cgactgcctg atttcaggca aggcgattcc
      841 caatgttgcc accatcatcg atggcataaa gttggagcag gggacgatgc agagcgtcaa
      901 gcgaccacgc tgtggcgctg cgtctgaaat gagagtgctg atgggatacc tttattttag
      961 aggaatagtc acgtaggcgc gcggctggtt gccgaccaca cgggtgatat gaccgcgccc
     1021 ggtggtgtcg tcggcgagca taatgtcgcc tttgccgaaa cgtttgacgg tgccgtcgcc
     1081 aacctcgatc tctacctcgc cggacaaagt gatgatgtac tggcgccgcg gcgctggatg
     1141 gaagtcgttt aagtgatggg tcggagcctg gcgaaaaaaa actccagtgg caggttccag
     1201 ctcggagact tccatatcgc cgtttaactt gagatcgatt tctttttctt cgaagtgcga
     1261 ttcgttgtct gctccggtgt aaattctcat gatcttcatg agtctggtat gtaggagaaa
     1321 gcgccgggaa aagcaaggcg aactccagac agagttctca gccggatctc agatttaacg
     1381 ttgcgcttgt gaactgtttg ccgcgcgtgt taatcagaaa atctccatgc ccgaacttcc
     1441 cgatatcacc gtttacatcg aagcgctgca agatcgattg ctgaaccaga cgctgcagaa
     1501 aatacgcata gcaagcccgt tcatattgcg ttcgttcgat ccgccgatcc agaccgcgga
     1561 aggaaaaaag gtgatcgggt ttgaccgctt aggtaagcgc atcgtcttcg aattggaaga
     1621 cgagctgttt atgatctttc accttatgat cgccggccgg tttcactgga aaaaggctgg
     1681 cgccccggtg ccgcggaaat tcggccacgc tgcgttcgat ttccctccgg gtactttgct
     1741 attgaccgag atgggcacca aaaagagagc atctcttcat ctggtccgcg gcagggaaca
     1801 cctagccgaa cacgatcctg gaggactgga aatattcggc gccgatctga aagcattccg
     1861 cgccgcgctg accgacgaga atcacacgct taagagaacc atgaccgatc ctcacgtttt
     1921 cagcggcatc ggcaacgctt actctgacga aattctgcat cgcgccggat tatcgccgat
     1981 caagcagacc caacatctca gcgaagtcga aatcgaaaga ctttatcgag caaccaaaga
     2041 agtgttacgc gagtggatca agagattgcg cacagacaca ggcaagaatt ttcccgaacg
     2101 ggttaccgcg ttccgtgatg acatggcggt tcacggcaag tatcgcaaac catgcccggt
     2161 ctgcggttcg ccggtgcagc gaatcgccta tgcggaaaac gaaaccaact actgcgcgaa
     2221 atgccaaacc ggcgggcgct tgctcgccga tcgttcgctg tcgcgcttgc tcaaagccga
     2281 ct
//
LOCUS       DASUTG010000807         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1904784, whole genome shotgun sequence.
ACCESSION   DASUTG010000807 DASUTG010000000
VERSION     DASUTG010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904784"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..959
                     /locus_tag="VF089_18745"
     CDS             543..959
                     /locus_tag="VF089_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6176056.1"
                     /translation="MKIDGSCHCGFITYEAEIDPEKVGVCHCTDCQSLTGSAFTTSVT
                     ADKQAFRLLSGQPKIYVKTAESGARRAQAFCPQCGSRIYAAAEIDPQTFNLRVGTIRQ
                     RRELRPKTQSWCRSALDWVMDLGSVPRNAQGRPRTQ"
     gene            complement(1060..2343)
                     /locus_tag="VF089_18750"
     CDS             complement(1060..2343)
                     /locus_tag="VF089_18750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6176057.1"
                     /translation="MKVYDISLSLDKMLGLLSAKRVLVYASVLALLTLITILALYIHV
                     PSFRESPGADFVQFYAAALLSGAEPDKIYDSQSQIEIQRQFSVGARNGVHWPYLHAPF
                     FTVLLIPLRWFSYVGAFWFWTLFTLTLYLLSVVLLIRSVPIRRPPLKLSLAVAAAAPV
                     FYWLILTGQTTAIALFIWTMAFVFLQQGRFYWSGFVLGFLSYRAQYLTIILPLLFLRR
                     MWWGMFGFSTSVVSLIVIGGLMFSFDSYLQYIEAVRQQSGRIENLTQPLSHYVTVYGF
                     FRQLLPPLWALYGTALASSILLYWLWKVWRGVVDFRSGIFDLQYASLVTTTLLLMYHG
                     FVYDLLLLTIPMLLLYQYRSMLPPYHVLVLLFLYFVPYMLLIFREQFLINPIQPALMF
                     LCFEIYVVSRTVVAEAADCNTRDLSHRVSLRDEKR"
     gene            2807..>2967
                     /locus_tag="VF089_18755"
     CDS             2807..>2967
                     /locus_tag="VF089_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX6176058.1"
                     /translation="MRYELYYWPGIQGRGEFVRLALEDAGAAYVDIARRPRGMSAMSK
                     FLESRSVKRP"
BASE COUNT          799 a          716 c          788 g          664 t
ORIGIN      
        1 cagccgccgg caaacgttgc tttggcaggc ggcgtcccgc cggggtttgg ctctgcgcca
       61 aacgcaaggc cttcgagaac tagaaagata accgcaactc ttgccagcgg ccttagaaga
      121 gtttcgcttc gcatgtattc ctccgagtgg ttaacttgcc gtctaagact gatcttagtc
      181 ggcggtaacc cgctctgcaa tctaagattg ttccagaagt ataggggtca cgcttctact
      241 tgaatttttg agggcacgaa gttcgagtgc gagcagatct cctggctaac cgggattagc
      301 ctataacgcc tctcccgagg tccgaacgtc cgccactgaa tgcatgctgg ttgggtcgcc
      361 gtggagagcc aagtaggatc ggaacggacg agcggtctga gaaagatggg aaatgagcgg
      421 cgctacattc attcgctaag ccactaaaag attgacacat tcttgtctgg acagtagaac
      481 cgtttcgttc tgatggtttt gagaaactga ttggcaaata aaaagcgcct ctggaggagt
      541 ttatgaaaat cgacggtagt tgccactgtg gctttatcac ctatgaggct gaaattgatc
      601 ctgaaaaggt tggcgtatgt cactgcaccg attgccaaag cctcaccggt tcagctttta
      661 cgacgtcggt cacggcagac aagcaggctt tcaggttgct cagtggccaa ccaaaaatct
      721 acgtgaagac cgcggaaagc ggcgctaggc gagctcaggc tttctgtcct caatgcggat
      781 cacggattta tgcagccgcc gaaatcgatc cacagacatt caacctgcga gtcggaacga
      841 ttcgccagcg acgggaattg cggccgaaaa cccaatcctg gtgccgctct gcattggatt
      901 gggttatgga tttgggttcg gtgccccgga acgcgcaagg cagacccagg acgcaataga
      961 gggaagagga ccggtacgcc aaaataaggc gcgcagtttc gtttctgcca acgtttccca
     1021 gattggaatg atcggctagc tggagttaga agccagaacc tatcttttct cgtctcggag
     1081 ggaaacccga tgagataaat ctctcgtatt gcaatctgcc gcctcagcga cgaccgttct
     1141 actcaccaca tagatttcaa agcacaagaa cataagcgcc ggttgaatcg ggttgatcag
     1201 gaattgctcg cgaaatatca gcagcatata agggacgaaa taaagaaata gcagcaccaa
     1261 tacgtgatag ggaggcaaca tcgatctgta ttgatacaga agcagcatgg gaatggttaa
     1321 gagcagcagg tcataaacga aaccgtggta catgagaagt aaagttgtcg ttaccaggct
     1381 cgcatactga agatcgaaaa tgccggatcg aaagtccacc acaccacgcc atactttcca
     1441 taaccagtat aacaagatgg acgatgccag ggcggtgcca taaagcgccc atagcggcgg
     1501 caacagctgc cggaaaaatc catagacagt tacataatgc gataacggtt gggtgaggtt
     1561 ctcgatccgt cccgactgtt gacgaaccgc ttctatatat tgcaagtacg aatcaaaaga
     1621 gaacattaaa ccaccgatca cgatcagcga tacaactgac gtgctaaagc caaacattcc
     1681 ccaccacatt cttcgcagaa atagcaacgg caggatgatc gtcaagtact gggctcggta
     1741 gctgagaaaa cccagaacaa atcctgacca gtagaatcga ccttgctgca ggaacacgaa
     1801 tgccatggtc caaataaaaa gagcgatcgc cgtggtttga ccggttaaga tcagccagta
     1861 aaataccggg gccgccgcgg ccacagctaa gctaagcttg agcggtggcc gtctgatcgg
     1921 caccgagcgg attaagagta cgactgataa gagatatagc gtcagggtaa aaagcgtcca
     1981 aaaccagaac gcgccgacat aggaaaacca tctgagcggt atcaaaagta cggtgaaaaa
     2041 aggcgcatgc agatagggcc agtggactcc attacgtgcg cccacgctaa actgccgttg
     2101 gatctctatt tgagattggc tgtcatatat tttgtccggt tccgctcccg aaagaagcgc
     2161 cgccgcatag aactggacaa aatctgcacc ggggctttcg cggaagcttg gaacgtgaat
     2221 atagagcgca agaatggtta tgagcgtgag cagcgctaat acggaggcat aaaccaaaac
     2281 acgtttcgcc gataacaaac ccagcatttt atccaggctc aaagaaatat cgtagacttt
     2341 catctcatct cggatcttat gtcagactga gaacaggcag ttttttcgat ggatgtcgat
     2401 tttgccggag attgcgctcg aaattaggtt cgctatttga atctgatgga ttcgattccc
     2461 cgcacacata ctctcttcga tggcgagcac caattttagc acgaatttct cgttggcagc
     2521 ttcatttttg acgtttggta ggtcatcgcc agtggagcgt tgctcttttg atctatgaac
     2581 ttccatcggg ctacgacacc gctagagtca gcgttttacg tcgtcgggac gattatcaaa
     2641 ggcgctttga tagagcgcta tagcgaagcc tttgcgattc tgcgggccgg agttgtttta
     2701 aattcatcaa ggagaagaac gatgcgacta tcatctaatc atcaagtatg tgcagtacga
     2761 cgtctgcggc cgctattcga gagcaagatc atttgcggag gagatcatgc gttatgagct
     2821 ttactactgg ccggggatcc aggggcgcgg tgagtttgtt cggctcgcct tagaagacgc
     2881 gggcgcggct tacgtcgata tcgcgcgacg cccgcgtggg atgtctgcga tgtcgaaatt
     2941 tcttgaaagc cggtcggtca aacggcc
//
LOCUS       DASUTG010000808         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1904896, whole genome shotgun sequence.
ACCESSION   DASUTG010000808 DASUTG010000000
VERSION     DASUTG010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904896"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VF089_18760"
     CDS             complement(<1..1295)
                     /locus_tag="VF089_18760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6176059.1"
                     /translation="MRTDIETNRPNDELLEIEKDESEELETDEPELELESDVATEENV
                     GEAEAQPEEKGLTVDPITTYLHEIGSIPLLSREREVELARKMDLGKNAIIEALFNAPM
                     CLHYTISLGDAVASGELDIREVLEKTDAEEDESRVSFDPKPFLRAISKLKRLRHEQDK
                     VRRQLNQKRVSEQRRSALNKAHVTLVNRIVGLLRDVGISSSRLESMVQKLKQSSKSIT
                     ELEERAHAVTKSKRAGIVAQIEQIEANVGLRAAEIHALASQITATESLVNLAKTEFTE
                     ANLRLVVSIAKKYTNRGLSLLDLIQEGNLGLMRAVEKFDYRLGYRFSTYASWWIRQGI
                     TRGLIDTGRMIRIPVHRVELRNKVIQTAHHLQRTLGREPKPEELAKEMDMSVPEMLRV
                     MQTQGEPVSLQTPVWEDGDQLADFVEDRINPQPDKEAMEG"
     gene            1480..1659
                     /locus_tag="VF089_18765"
     CDS             1480..1659
                     /locus_tag="VF089_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176060.1"
                     /translation="MAIIIKVKYKTTGIAAWFIALFWRLVGRVAGIVKSKDPHAPCQA
                     KAVLRNLYLEISCMK"
     gene            2204..2920
                     /locus_tag="VF089_18770"
     CDS             2204..2920
                     /locus_tag="VF089_18770"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6176061.1"
                     /translation="MKAQEWRHELGECVRYLFRSPEMEHFYSIKMTTQRARIYLLQLS
                     LYVRQRRNFWPQVAANCPEFDVKQRIMSHEYEELVEDEHSKHGHLDLIFRQGKEVGLS
                     LDEIVNAEPLPTTTAAIYAWFWIARNRPWQEAISASTIAEWTNDDRLLGDLGGGNCSR
                     LYKIWKNDLNLSDMQMPNFTAHAKADVKHSDMFIDVLEKYVAPGREQDVLKTAKESMD
                     IHRAYFGGMAQTMAKALEAA"
     gene            2930..3946
                     /locus_tag="VF089_18775"
     CDS             2930..3946
                     /locus_tag="VF089_18775"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176062.1"
                     /translation="MKLAELDGQLEKNQLAGFWNVRVPGHSAEAPYLWKWEPLLDGLL
                     KASETIGIEQAERRAIRLVSPHIPNKSTSHTVQLTFSIVNPGEVARAHRHNMAAIRFV
                     VQGKGAYTAVQGEKFPMEEGDLVLTPNWTWHEHHNESEDPIIWLDGLDGPLIQALNVL
                     FFEQSERHEQPITHSTGDSLSRFGFARTPKVDNTHLRGVPFRYAWKETYKALKLMGRS
                     EQDPYDGTLLRYINPVTGGYTYPTMSCEIQLFEPNCITHVHRHTSTAVYHVFKGRGRT
                     AVGESYLEWKKGDSFVIPLWQWHSHENPFDEEAILFSINDRPVMETLQLYREEFSNGN
                     KNAR"
     gene            4072..5076
                     /locus_tag="VF089_18780"
     CDS             4072..5076
                     /locus_tag="VF089_18780"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX6176063.1"
                     /translation="MASTTAIRIASRTYDGTLPILRGQMQVPGFDLHITETSDVAGMF
                     AGMFKGQYDVGEMSLGELIYYTSRAKADFIGIPVFPSRMFRHGFIFCRRSSGVNKPGD
                     LSGKSIGFLRWVQTAAIWMRGMLIDEYHVSPSATRWYIASMHHWDDADPNASVEPRDG
                     SIIHPIQSAGKNASERACRALFDGEIDVLGVTESQLPMLLANDAVGRLFVDPRQVEVS
                     YFQRTRILPIMHVVALRKDLAGQHPELPQKLFHLYSDAKRWAQRWRRTIPSLVEAWPN
                     HYLSEEKEIFKTDPWAYGLEANRHVLAKFLAYCYEQGVSAKSIAPDELFCPSTLRLVE
                     "
     gene            5107..>5512
                     /locus_tag="VF089_18785"
     CDS             5107..>5512
                     /locus_tag="VF089_18785"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176064.1"
                     /translation="MSTALASKSQRVAACDLRDWLREVEATGQLASISGAHWDLELGA
                     LTEIILERMSTPPALMFDSVPGYEPNRRVLANMLESLERTALTLNLPIGLETIPLIDA
                     LRARLRALQPIKPNIVSTGAVMENVERGDQVDL"
BASE COUNT         1296 a         1437 c         1413 g         1366 t
ORIGIN      
        1 ccttccattg cttctttgtc aggttgagga ttgatccggt cctcaacgaa atcggcgagc
       61 tgatcaccat cttcccaaac cggcgtctgc agcgagacag gttctccttg tgtttgcatc
      121 actctaagca tttctggaac cgacatgtcc atctctttcg cgagttcttc cggcttaggc
      181 tcacgcccta gggtccgctg tagatgatgg gcggtctgaa taactttgtt ccttagttca
      241 actctgtgga ccgggatgcg aatcattcgg ccggtatcga tcaaaccgcg cgtgatccct
      301 tgtcgaatcc accaacttgc ataggtagag aaccggtacc ccaaacggta gtcaaatttc
      361 tcaacggctc tcattagccc aaggttacct tcctgaatca ggtcgagtaa gctcaggccg
      421 cgatttgtat acttcttggc gatgctgact acgagccgca gatttgcctc cgtgaattcg
      481 gttttggcca ggtttaccag actctcggtc gccgtaatct gtgaggccaa ggcatggatt
      541 tcagctgcgc gaagtccgac gttggcttcg atctgttcga tctgagctac gatgccggcg
      601 cgcttcgact tggtcacggc atgtgcccgt tcctcgagct ctgtgatgct tttggacgat
      661 tgttttagtt tctgcaccat ggattcgaga cgagaagacg aaatcccaac atcccgcagc
      721 aggccgacga ttctgttcac caaagtcacg tgggctttgt tgagggcgct ccgacgctgt
      781 tccgatacac gtttttgatt cagctgccgg cgaaccttgt cctgctcgtg cctgagacgc
      841 tttagcttgc ttatagctct cagaaaaggc ttgggatcga agctgactcg gctctcgtct
      901 tcctcggcat ccgttttttc tagaacttct cggatatcga gttcaccact ggccacggca
      961 tcgcccaagc tgatagtgta gtgcaggcac attggcgcat tgaacagggc ctcaataatc
     1021 gcattcttgc ccaaatccat cttcctggcg agctcgacct ctcgttcccg gctaagtaac
     1081 ggaatggatc cgatttcatg taagtaggtt gtaattgggt cgactgtcag tcctttttct
     1141 tcaggctgag cttcggcttc cccgacattt tcctccgtcg caacgtcgga ttcgagctcc
     1201 aactccggtt catccgtttc tagctcttct gattcatctt tttcgatttc caacagttca
     1261 tcgttggggc gattggtttc aatatctgtc cgcatggttt ctcctcaata gtaactagat
     1321 agacgatacg tagacgataa accgtcactt cttaagcccg gcggctctct ttaaggacgc
     1381 cctttgaccc ttctagccct aaaagcaaag cgaattcggt gccatatgcc gcccttggtg
     1441 tttggccata attcaccgac ggattctcgt aaatgagaca tggcaatcat catcaaggtg
     1501 aaatacaaga ctacagggat tgcggcttgg tttattgcgc tgttctggcg gctggttggt
     1561 cgcgtcgcgg gcatcgttaa atctaaggat cctcatgctc catgtcaagc taaagcagtg
     1621 ctgcggaatc tgtatcttga gatttcctgt atgaagtgac tgttactctg gcggcgggtc
     1681 tgcgcttggt gactgcggcc cagatttacg agatccccaa ttggctaaac catgagccac
     1741 atctacaaac ctccttgcgc gatgggacgc aactgggctg ctgcggccgg tcgtgctcgc
     1801 acatctgaac taggactttc cgctggttta ttgagaagcg gcgtacaaac gaaggctctg
     1861 ccagcgtatc ggagcattcg ccggtgagca ggagccacga tttgatcaat gaggcatcat
     1921 tttccaccat aagaatcaac aataagtacg ccctgcgctc attgagagtc agtggacctc
     1981 ccaagcgccc gagaccagaa tcgaatccgg attttttggc gcaagggcgc cgcatagacc
     2041 tgatagagat gcgacattat cctcgaaact gattgtaagc ggtcgaatac ttgacaatgc
     2101 tttgaaggat cggctagtgc ttacaactga ttacttcgca taaccgtagc ggagcgtcgc
     2161 agttgcaggt agtttatcga tgcaccccta aggaggagcc tccatgaaag cacaagaatg
     2221 gcgtcacgaa ttaggtgaat gcgtccgtta cttgtttcgc tctcctgaaa tggaacactt
     2281 ctattctatt aagatgacca cgcaacgtgc acgaatttat ctcttgcaat tgagcctcta
     2341 cgtacggcag cggcggaact tctggcctca agtggctgcg aattgccccg agttcgacgt
     2401 aaaacagcga atcatgtctc atgagtacga agaactagtt gaggacgagc actcgaagca
     2461 tggtcatctc gatctgatct ttcgccaagg caaggaggtt gggctgagtc tagacgagat
     2521 cgtcaatgca gaacccctcc cgaccacgac agcggcgatt tacgcatggt tttggatcgc
     2581 ccggaatcgt ccctggcagg aggcaatctc cgcgtcgact attgcggaat ggactaacga
     2641 tgatcggtta ctgggagacc tcggcggcgg taattgcagc cgtttataca aaatctggaa
     2701 aaacgatcta aacctgagtg atatgcaaat gcccaacttt acagcccacg ccaaggctga
     2761 tgttaaacat tccgatatgt ttatagatgt actggaaaaa tacgtcgcgc ccggacggga
     2821 gcaagacgtg ctgaaaacgg cgaaagaatc tatggacatt catcgggcct acttcggcgg
     2881 aatggctcaa accatggcga aagcgttgga agcggcatag gagcggagta tgaaacttgc
     2941 cgagttagac ggccagttag aaaaaaacca actagccggc ttctggaatg tccgtgttcc
     3001 gggtcattcc gcagaagcgc cctatctatg gaaatgggag cccctgctgg acggcttgtt
     3061 gaaggcaagc gagacaattg ggatcgagca ggccgagcgt cgcgccattc gtctggtaag
     3121 cccccacata ccaaataaat ctacctctca caccgtgcag cttacattct caatcgtcaa
     3181 cccgggagag gtcgcgagag cgcaccgcca taacatggct gccatccgct tcgttgtcca
     3241 gggcaagggc gcttatactg cggttcaggg tgagaaattc cctatggaag agggtgattt
     3301 ggttctgacc cccaactgga cctggcatga gcatcacaac gaatcagaag atccaatcat
     3361 ctggctcgat ggtctggacg gtccattgat ccaagcgcta aacgttctat tcttcgagca
     3421 gtctgaaaga cacgaacaac cgatcacaca ctccactggc gactcgctaa gccgctttgg
     3481 ctttgcacga acgccaaaag tagataacac gcacctgcgt ggtgttcctt tccgatatgc
     3541 gtggaaggaa acgtacaaag cgctcaaact tatgggcaga agcgagcagg atccatacga
     3601 tgggactttg ctgcgttaca tcaatccagt caccggcggg tatacttatc caaccatgtc
     3661 ttgcgagatc cagctttttg agccgaactg cataacccac gtacataggc ataccagcac
     3721 ggcagtctat catgttttca aagggcgggg ccggaccgcc gtaggagaaa gttatctcga
     3781 gtggaagaag ggcgattcct tcgtgattcc gctttggcaa tggcattcgc acgagaatcc
     3841 attcgatgaa gaagccattc ttttctcgat taatgaccgc ccagtgatgg aaacattgca
     3901 gctttatcgc gaagagttca gcaacggcaa caagaatgcg agatgaagta cattcttagc
     3961 actcatgggc atgaacttcc tccgccgttc tttcctagag agcgccggcg actcgtgcgc
     4021 agcggtagtt caccacaaga cattataaac taactccgcc tggctaactg aatggcttct
     4081 acaacagcca taaggatcgc gagtcgcact tacgatggca ctctgccgat cctgcgcggc
     4141 caaatgcagg ttccgggatt cgacctgcat atcaccgaga ctagcgatgt cgctggtatg
     4201 ttcgccggaa tgttcaaagg gcagtatgac gttggtgaaa tgtcgcttgg cgaactgatt
     4261 tactatactt caagagctaa ggccgatttt atcggcattc cagtcttccc ttccaggatg
     4321 tttcggcacg gttttatttt ttgccgccgc tcttcgggcg tgaataaacc cggcgatttg
     4381 agcggtaaaa gcataggatt tctgcgctgg gtgcaaacag cagccatatg gatgcggggc
     4441 atgcttatcg atgaatacca cgtttcgcct tcagctactc gatggtatat cgcttcgatg
     4501 catcattggg atgatgcgga tccgaatgcc agtgtcgaac cgcgcgatgg ttcgatcatt
     4561 catccgatcc aaagcgcggg gaagaatgca tctgaacgcg cctgccgcgc tttattcgat
     4621 ggcgaaattg acgtcctcgg agtaacagaa tcgcagctgc caatgttgct ggcgaatgat
     4681 gcggtcggcc ggctcttcgt tgacccacgt caggtggagg tcagctattt tcagagaacc
     4741 agaattttgc cgattatgca tgttgtcgca ttgcggaaag atttggctgg acagcatccg
     4801 gaactgccac agaaactttt tcacctctac tccgacgcca aacgatgggc gcagcgctgg
     4861 cgccgcacta tcccaagtct ggtcgaggct tggccaaatc attatttgtc cgaagaaaaa
     4921 gagatcttta aaaccgatcc ctgggcgtac gggttggaag ccaatcggca cgtgctcgct
     4981 aagtttctcg cctactgtta tgagcaaggc gtcagcgcca agagcattgc gcctgatgag
     5041 ttgttctgtc ctagtacctt gagactcgtc gaatgagttt ccgcacgtga acaaaaggag
     5101 ttctcgatgt ctactgcact agccagcaaa tctcaaagag tcgccgcctg cgatctacgc
     5161 gattggctca gagaagtgga agccactgga caattggcaa gcatctctgg ggctcactgg
     5221 gatttggaac taggtgcgct cactgagatt atactcgaac gaatgtctac gccgccggcc
     5281 ctaatgttcg attctgttcc cggttacgag cccaatcggc gagttctcgc caatatgctg
     5341 gagagcttgg aacgcactgc actcactctt aatctgccga tcggtcttga gacaataccc
     5401 ttaattgatg ctctgcgtgc aaggctacga gcccttcaac cgatcaaacc aaatatcgtc
     5461 tccactggcg ccgttatgga aaatgttgaa cgcggtgatc aggtcgatct ct
//
LOCUS       DASUTG010000809         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1458945, whole genome shotgun sequence.
ACCESSION   DASUTG010000809 DASUTG010000000
VERSION     DASUTG010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458945"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF089_18790"
     CDS             <1..495
                     /locus_tag="VF089_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6176065.1"
                     /translation="FDKWITVLRASQRYVRQIPDPRLAERAIENRDRSIRLLSHHIFR
                     IGEAFLETALEDVEYRTNNANVPPADGTCLTGVEIAGYGDGIIERIENWWTRLEDKSC
                     RQQVRTFYGTQPMHQLFERSAWHSAQHARQMIAVLERFGIEPDSRLTAEDLAGLPLPE
                     KIWE"
     gene            875..2143
                     /locus_tag="VF089_18795"
     CDS             875..2143
                     /locus_tag="VF089_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176066.1"
                     /translation="MPKHAESAAAQSVAAPAKPVAFAALRHRDFRFFFPATMLAMMAD
                     NIEHVISYWLLFEKFQSPVLAGFAVISHWTPFLLLSVYFGGLADRYDCRKLIQIAQVM
                     YMLVSIAWAILFFTDSLEVWHACVLLVIHGIAGVFWTPAEQLLIHDIVGPEDLPSAVR
                     LNSTSRQLGILLGPAVGGAMMLWLGAPAGLLINAIIYLPLTIWLMIVPYTGHLRDDAP
                     PRRAMGWKDAIGTVRQIMEHRPIVTMIALGGAASLFVGNAFHAQMPEFAHDLGTEKAD
                     LTYSALLAANAAGAVVGGFVLDGKDWLPPTVRSAILCAILWCVVMAAFAFSYSYPLSL
                     VLLFGAGALNLAFYSTAQTIVQLQAPIDLRGRLIGLFTMSAYGLRAFSGVTVGVVGGL
                     IGIHWSLALSSMILLGITLALFAFALPAQK"
BASE COUNT          387 a          653 c          650 g          532 t
ORIGIN      
        1 ttcgacaagt ggatcaccgt gctgcgcgca tcgcaacgct acgtccgcca aattcccgac
       61 ccgcgactcg cggagcgagc tatcgaaaat cgcgaccgct cgatccggct cttaagccat
      121 cacatcttcc gcatcggcga agccttcctc gagaccgcgt tggaggacgt cgagtaccgc
      181 accaacaatg ccaacgtgcc gcccgccgac ggaacctgcc tcaccggagt cgagattgca
      241 ggttatggcg acggcattat cgaacggatc gagaactggt ggacccggct cgaagacaaa
      301 tcgtgccggc aacaggtccg gacattttat ggcacgcagc cgatgcacca acttttcgag
      361 cgctcagctt ggcattccgc gcagcatgcg cggcaaatga tcgccgtcct cgagcgcttc
      421 ggcatcgaac ccgacagccg gttaaccgct gaagatcttg ccggattacc tctcccggaa
      481 aaaatttggg agtagagcat ccgggtccca ctccttagtt caaaaatccc gcgcgatcct
      541 cggtttcaag gtgatgggca cgaggtccag gacaaggtca catgcgctac ctgacatcgt
      601 tgaattcctg gcgcgctatc tcctgttgac gatgagcctc gcttcgactc cgttccgcac
      661 ggtggggacg ttctgaccga tctctcgggt tgagagcatt cgctgggcag tctgcgccca
      721 tcctgtgaaa tcaacggcag tcccgtcccg aaaggttact tgttgatcga gagattgccg
      781 atctcgcctc ctgctgattg catagggttc caagattggc tactgtcgaa agttctacaa
      841 tctgctgcaa accgcgttca tttctggagg aaaattgccc aagcacgccg aaagcgcagc
      901 cgcgcagagt gtagccgccc cggctaaacc ggtggctttc gctgcgctgc gtcaccgcga
      961 ttttcgtttt ttcttcccgg ccaccatgct cgccatgatg gcggacaaca tcgagcatgt
     1021 gatcagttac tggctgttgt ttgagaagtt tcagtcgcct gtgctggcgg gcttcgcggt
     1081 gatcagtcac tggacgccgt ttctcttgct ttctgtttac ttcggcggct tggccgatcg
     1141 ctacgattgc cgcaagctga ttcagattgc ccaagtcatg tatatgttgg tctctatcgc
     1201 ttgggcaatc ctctttttca cggatagtct cgaggtttgg cacgcctgcg tgttgctggt
     1261 gattcacggc atcgccgggg tcttctggac gcccgccgag caactgctga ttcatgacat
     1321 tgtcggcccc gaagatctgc cgagcgcggt gcggctcaac tcgacaagcc ggcagttggg
     1381 gattttgctc gggccggcgg tgggcggcgc catgatgctc tggctcggcg ccccggccgg
     1441 cttgctcatc aacgcgatta tttatctgcc attaactatc tggctgatga ttgttcctta
     1501 caccggtcat ctccgcgacg atgcgccgcc gcggcgagcg atgggttgga aggatgccat
     1561 cggcacggtg cggcagatca tggagcaccg gccgattgtg accatgatcg cgttgggcgg
     1621 cgctgcctcg ctatttgtcg gcaatgcatt tcacgcccag atgccggagt ttgcccatga
     1681 cctcggcacg gaaaaagcgg atctcacgta cagcgctttg cttgctgcca atgccgcggg
     1741 ggcggtagtg ggcgggttcg tgctcgatgg caaggactgg ctgccgccga cggtgcggtc
     1801 ggcaatcctc tgcgcgatcc tgtggtgcgt ggtgatggcg gctttcgctt tttcctacag
     1861 ctacccattg tcgctggtgt tgttgtttgg cgccggggct ttgaatctcg ccttctactc
     1921 caccgcgcaa actatcgttc agctgcaggc gccgattgat ttgcgcggcc ggctgatcgg
     1981 tttatttacc atgtcggctt atggcctgcg ggccttcagc ggggttaccg tcggcgtggt
     2041 cggcggcttg atcggcatcc actggtctct ggcgctgagc tcgatgattc ttctggggat
     2101 taccctggca ttattcgcct tcgcgttgcc tgcgcaaaaa tgacggtgct gtgccgtcag
     2161 acgatttcgc ttcagcgctg tcgattaaca cgttatctga agccgcgacg gctgatcgtt
     2221 gc
//
LOCUS       DASUTG010000810         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_568897, whole genome shotgun sequence.
ACCESSION   DASUTG010000810 DASUTG010000000
VERSION     DASUTG010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568897"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..833)
                     /gene="tolQ"
                     /locus_tag="VF089_18800"
     CDS             complement(93..833)
                     /gene="tolQ"
                     /locus_tag="VF089_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX6176067.1"
                     /translation="MFSFWSLHLLAQNGSFVPQPHGILDLVRGSGIVVQAILYLLVLF
                     SVVGWGIIAYKFRQMRRAKKESEKFIEIFWERRNLSSIHDASRELKSSPVGQVFRSGY
                     EELVRVSRSKKDSPSGDNLTTELGGVDNVSRAMKRATSVEITKLEKYCSFLATTASAA
                     PFIGLFGTVWGIMNAFMGLSTTHSSSIQAVAPGIAEALIATAVGLAAAIPALMAYNYF
                     VQQIKVLAVEMDNFSHEFLNIAERHFFK"
     gene            938..2116
                     /locus_tag="VF089_18805"
     CDS             938..2116
                     /locus_tag="VF089_18805"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6176068.1"
                     /translation="MICRVDQLDITGKTVFLRVDFNVPLDKGKITEPHRIESAVPTIR
                     LLLEKSKKVIIASHLGRPGGRKDPKFSLAPIRAYLEKSLGQTVVLAPDCIGSEVEELA
                     GDPDSRVLLLENLRFHPEEEKNDASFSRALASLADVYVNDAFGAAHRAHASTTGMAEF
                     FELKAAGLLLQRELDYLSRVLTGPEKPFIAILGVAKVSDKIGVIKNLLPKLDALLIGG
                     GMAYTFLKADGISIGRSLVEKDKVSVAKELMSDAMTHSVKVLLPTDHVTGDENKENSS
                     ITDVNIPEERVGLDIGPRTAQRFVEEIGRSKTVLWNGPVGLFEVEPFDQGSKALAQAL
                     ADQRRNVVSIVAGGDTVAAVTAAGVADHITHLSTGGGATLEFLEGRELPGIKALEKTA
                     "
     gene            2113..2868
                     /gene="tpiA"
                     /locus_tag="VF089_18810"
     CDS             2113..2868
                     /gene="tpiA"
                     /locus_tag="VF089_18810"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6176069.1"
                     /translation="MNEPLVVGNWKMNGTLAQASSLADAILHGIAQRAPKAALVLAPP
                     ATALVALQQRIAKSGISLAGQNCHWEDTGAFTGEISPAMLSDLGCTYVIVGHSERRHI
                     FNESDEIVARKLAAAIRNNLRPILCVGETLDERNAGRTMEVIMRQMQLALKDPNEDVI
                     ANLEVAYEPVWAIGTGHNADPSQIVEVHGAIRNYLTSSFGKTAFRILYGGSVNAENAS
                     DLGKTSEVNGLLVGGASLKPESFLAVVRCFDDE"
     gene            2875..>3164
                     /gene="secG"
                     /locus_tag="VF089_18815"
     CDS             2875..>3164
                     /gene="secG"
                     /locus_tag="VF089_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6176070.1"
                     /translation="MVIAVTILHIVVCIGLILVVLLQTGKGAEIGAVFGGGSSSTIFG
                     SSGAGNFLTKLTTAMAIVFMITSLTLGYFAGQRPSATIFDSRSPASQPGAPLA"
BASE COUNT          798 a          777 c          833 g          756 t
ORIGIN      
        1 atgacgtcaa caagtggggt aacgttaatc tgcgaaatcg tcgtgctgtc acgcgatgag
       61 gcggtgtcca ttgccatctg aacctctttt gattatttga agaagtggcg ctcagctata
      121 ttgagaaatt catgagaaaa attgtccatc tcaacagcca gcaccttgat ctgttggacg
      181 aagtagttgt aggccatgag cgccggaatc gcggcagcca agcctacggc agtggcgatc
      241 aaagcttcag caatcccggg cgccacggct tgaatgctcg aagaatgtgt cgtgctcaag
      301 cccatgaatg cgttcataat cccccacacc gtgccaaaaa gtccaatgaa cggcgccgcg
      361 ctggcggttg tggcaaggaa gctgcaatat ttttccagct tcgttatctc cacactggtc
      421 gcacgcttca tggccctgga aacattgtcg acgccaccta actcagtggt caagttgtca
      481 cccgatgggg aatccttctt tgagcgagat acccggacaa gctcttcata accggagcga
      541 aagacctggc ctaccggact agatttcagc tcccgactgg catcgtgtat cgatgacaag
      601 ttgcgtcttt cccaaaaaat ttcgatgaat ttttccgact cttttttagc cctccgcatc
      661 tgccggaact tgtaggcgat aataccccat ccgacgacgg aaaatagaac cagcaggtaa
      721 agaatcgctt gaacgacgat tcctgatccc cggacgagat cgagaatccc atgcggttgc
      781 ggtacaaacg agccattttg ggctaaaagg tggagcgacc agaaagaaaa catgtcgggt
      841 ttttgaaatg ctatttgaaa aaatattata acctctgtta ttagtcaatt agtaaatgcg
      901 tatttctcaa cattaatgtt caatctggga agtaactatg atttgccgcg ttgatcaact
      961 cgatattaca ggaaagacag tattcttacg ggtcgacttc aatgtgcctc tcgacaaagg
     1021 caaaatcacc gaaccgcatc gcatcgaaag cgccgtgccg accatccgac tgctccttga
     1081 gaagtcaaaa aaggtgatca tcgcctcaca tctgggccgt ccaggcggta ggaaggatcc
     1141 aaaatttagc ctcgccccaa taagggcgta cctggaaaag tctctggggc aaaccgtagt
     1201 tctggcgccg gattgcattg gctcagaggt tgaggaactc gcgggtgatc cagactcgcg
     1261 agttcttctg cttgaaaatt tgcgctttca tcctgaagag gaaaagaatg acgcgagttt
     1321 ctcccgtgcc cttgcatcgt tggccgatgt gtatgtcaat gatgcattcg gtgctgccca
     1381 tcgtgcccat gcttccacga caggtatggc ggagtttttc gagctgaaag ctgcaggcct
     1441 tttattgcaa cgcgagctcg actatctctc ccgcgtgctg acgggccccg aaaagccttt
     1501 catcgcgatt ctgggtgtag caaaagtttc agataagatc ggggtgatca aaaatttatt
     1561 acccaaacta gatgcattgc tcatcggcgg aggaatggcc tacacgtttc tgaaagctga
     1621 cgggattagc atcgggcggt cgctggttga aaaagataaa gtttctgttg caaaggagct
     1681 gatgtccgac gcgatgacac acagcgtcaa agtactcctg ccgacagatc atgtgactgg
     1741 agatgaaaac aaagagaatt cttctatcac tgatgttaac atcccagagg aacgtgttgg
     1801 attggatatc ggaccacgaa cagcacagcg gtttgttgag gagatcggca gatccaaaac
     1861 tgtcctctgg aatggtcccg tcggactctt tgaagttgag cccttcgacc aaggaagtaa
     1921 agctcttgcc caagcactgg cggatcagcg gcgtaatgtc gtcagtattg tagcgggcgg
     1981 ggacacggtt gccgcagtca cggctgcggg agtggctgat cacattactc atctttcaac
     2041 cggaggcggc gcgactttgg agttcttaga aggacgtgag cttcccggca tcaaggcact
     2101 tgagaaaact gcatgaacga gcccctggtt gtcggcaact ggaagatgaa cggaacgtta
     2161 gcgcaggcgt ccagccttgc cgacgccatt ttgcacggaa tcgctcaaag agcaccgaaa
     2221 gctgctttgg tactggcgcc cccggcaaca gcgttggttg cgttacaaca aaggatcgct
     2281 aagtccggca tctctctcgc cggccagaac tgccattggg aagataccgg cgcgttcacg
     2341 ggcgaaatca gcccggctat gttatcagat ttgggctgta catatgtgat cgtaggccac
     2401 tcagagcggc gccacatttt taacgagagc gatgaaatcg tcgctagaaa gttagccgct
     2461 gcgatcagaa acaatcttcg cccaatcctt tgtgttggtg aaactctcga cgaacgcaac
     2521 gccggcagga ccatggaggt tatcatgcgg cagatgcaac ttgcgttgaa ggacccgaac
     2581 gaagatgtta tagcaaacct cgaagtcgct tacgagccag tatgggctat cggaactggt
     2641 cacaacgcag atcccagtca gatcgttgaa gtccatgggg cgattcgcaa ttacctgacg
     2701 agttcgttcg gcaaaactgc ctttcggatc ttgtacggcg gtagtgtaaa cgctgaaaac
     2761 gccagcgacc tcggtaaaac ttcagaagtg aacggccttc tcgtaggagg cgcaagctta
     2821 aagccggaaa gctttctcgc ggtagttcgt tgcttcgatg acgagtaaaa aattatggtg
     2881 atagcagtta ctattttaca cattgttgtt tgtattggat tgatattggt tgtgttgctg
     2941 caaaccggca aaggagctga aatcggcgcc gtattcggcg gtggttcgag ctcgactatt
     3001 tttggcagca gcggtgcagg aaacttttta accaagctta caacggcaat ggctatcgtc
     3061 tttatgatca cttctctgac tttgggatat ttcgccgggc aacggccatc ggcgaccatc
     3121 tttgatagtc gcagtcccgc atcgcagccc ggcgctcctt tggc
//
LOCUS       DASUTG010000811         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1015432, whole genome shotgun sequence.
ACCESSION   DASUTG010000811 DASUTG010000000
VERSION     DASUTG010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015432"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..415)
                     /locus_tag="VF089_18820"
     CDS             complement(89..415)
                     /locus_tag="VF089_18820"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6176071.1"
                     /translation="MTPMIRLAVLLVVLFAVTGCQAMTGRTAGRNIDDLTITASVKAR
                     LVADKPSNLTRIDVDTVNAVVSLTGLVESEFEKRRAEDIASLVDGVKQIVNDLQVQKE
                     PAPKSE"
     gene            749..1147
                     /locus_tag="VF089_18825"
     CDS             749..1147
                     /locus_tag="VF089_18825"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6176072.1"
                     /translation="MSQENITVIRGMYEFFSKGDVTAVLGQMHQQIEWREAENFIYAD
                     RNPYRGPQAVLEGVFMRLASEWADFKVMPEEWLDAGNHIVVLGTYSGRHKESGKEVRA
                     QFAHIWGVTHGRVVRFQQYTDTKQFADAIA"
     gene            1294..2292
                     /locus_tag="VF089_18830"
     CDS             1294..2292
                     /locus_tag="VF089_18830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176073.1"
                     /translation="MRTLFIAAVFFVSLFESGAAQAQSKIYVGLSTLNSRVTPLWIAQ
                     ERGFFAKNGLEVLLVLTRVSQPAIAALLAGEMQMVYGGSTAALGAVSSGADLKIIAAL
                     TNRLTYDLVARPGINRAEELRGKRLGVGSIGGTVWMGAMLGLEHLGLDPSRDRISILV
                     VGDQTNTGQALEAGSIDAALLDGVFTRRLRQKGFPVIAEFAGANIPFAGQGIVTSGPY
                     LMKQAPVIENFLKGLLEGIDFSLAPANKASVLKTMTARLRLPDAASAAVGYQDLIRGV
                     EPKPYPSLDGLRNLQRLMRVHNPKVGGIRVEEMVDDRILRRIIERGFIDAVRSSNR"
     gene            2362..>2651
                     /locus_tag="VF089_18835"
     CDS             2362..>2651
                     /locus_tag="VF089_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176074.1"
                     /translation="MANYKVMDADGHVRESIAGMREFLEPRWHRRNLFPNDEWDRDLH
                     GKLGQKPEGPEDQLSAMDQDGIDVMVLYPTAGLHVSALHEKDYATAITRAYN"
BASE COUNT          624 a          666 c          754 g          607 t
ORIGIN      
        1 cggaagttcg gtggatccag gaccttacag cgtcctaaga aggtttgagg ccaatggtca
       61 ctctcgcttg ctttttcacg agttaggatc actccgactt aggcgcaggt tctttctgca
      121 cctgcaggtc gtttactatc tgctttacgc cgtctaccaa ggaagcaatg tcttccgccc
      181 gccgtttctc aaactccgat tcaaccagcc cggttaagga aacgaccgca ttcaccgtat
      241 ccacatcgat gcgagtgaga tttgacggct tgtctgcaac aagccgcgcc ttaactgatg
      301 ccgtgatcgt caggtcatcg atattacgac cagcggttcg tccggtcata gcctggcaac
      361 cggttacagc aaaaagtaca actagcaaaa ctgccaatct tatcatgggt gtcattcggt
      421 atagttcctt tccacaacga gcgataatct aacagttgct ccttcttctc gaaaacagca
      481 cgcatcttta gctgagacca atcgatttcg catgaaagtg aaatcgcgac ggctttgcct
      541 gtcacgcaga ggttgagact gtcggcgtca ttttcaactc tcatttaatt gcgtggaata
      601 gaggtggcag gatcttcggt tgagagactg acgatcgtgt ttgaaagctg cgcaagaggt
      661 tgcggatgtt tctgatatcc actaggagaa tctataggag atttttacgg cccatgagcg
      721 aaaaatctgt gcgttcaagg aggccaatat gtcacaagag aatatcactg tgattcgcgg
      781 catgtacgag ttcttcagca agggagacgt taccgcagtt cttggccaaa tgcaccagca
      841 gatcgagtgg cgcgaggcag aaaacttcat ctacgccgat cgtaatcctt atcgcggccc
      901 acaggcggtg ctcgagggtg tcttcatgag acttgcttcg gaatgggcgg acttcaaggt
      961 gatgccggaa gaatggctgg atgccggaaa tcacattgtg gtgctcggca cttacagtgg
     1021 caggcacaaa gaaagcggga aagaagtccg cgcgcagttc gctcatatct ggggtgtgac
     1081 ccacggcaga gttgtgcgat tccagcagta taccgatacc aagcaatttg ccgatgccat
     1141 tgcttagctc gggtgattgt agcagtcaga ccaggatacg accggctgcg attgcgaggt
     1201 agctttggca tgctgcattg cggtatggcc aatgcgcatt gacatcaagg acccagttgc
     1261 agcgcaaaat aatcagctga tgatccaaag accatgagaa cgctgttcat agcggctgtc
     1321 ttcttcgttt ctttgtttga atccggcgca gcgcaagcgc agagcaagat ttatgtcggc
     1381 ctttccacgt tgaattctcg agtgactccc ttgtggatcg cccaggaacg gggattcttt
     1441 gcgaaaaatg gcctcgaggt gctgctggtg ctgactcgag tgtcgcaacc agcgatcgcc
     1501 gcattgctgg cgggagaaat gcagatggtc tacggcggct cgaccgccgc gctcggtgct
     1561 gtttccagcg gtgcggattt aaagataatt gcggcactga cgaatcgttt gacctatgac
     1621 ttggttgcaa gaccaggcat caaccgagcc gaggaacttc gtgggaagcg gcttggagtg
     1681 ggcagtatcg gcggtaccgt ctggatgggt gccatgctag gactcgagca cctgggtctc
     1741 gatccgtcga gagatcggat ctctattcta gtagtcggag atcagaccaa caccggccaa
     1801 gctcttgaag caggaagcat cgatgccgcc ctcttggatg gagttttcac gcgccgcctc
     1861 cgacaaaagg ggtttcccgt aatcgccgag tttgccggcg ccaacatccc ttttgctggg
     1921 cagggaatcg tgacgtccgg cccatacctg atgaagcaag ccccggtaat cgagaatttc
     1981 cttaagggct tgctggaagg catcgacttt agcttggctc ccgccaacaa ggcgtcggtg
     2041 ctgaaaacaa tgacggcgcg ccttcgtctt ccggatgccg ccagcgctgc agtcggttat
     2101 caggatctga ttcgcggcgt cgaaccgaaa ccgtatcctt cgctggacgg attgcgtaat
     2161 ctgcaacgcc tgatgcgagt ccacaatccc aaagtcggag gcattagagt cgaggagatg
     2221 gtggatgatc ggattctgcg gaggattatc gaaaggggct ttattgacgc tgttcgaagt
     2281 tcgaatcgct agatggcacc cgggtccgaa tatttagcgt caatggggtg cattaaaacc
     2341 gacggaagag aaggaggaga aatggctaac tacaaagtga tggatgccga tggccacgtg
     2401 cgcgaatcga tcgctgggat gagggaattt ctagagccgc gctggcaccg ccgtaatttg
     2461 tttcccaacg acgagtggga ccgcgatctg cacggcaagc tcgggcaaaa acccgaagga
     2521 ccggaagatc aactgtccgc catggaccaa gacggcattg acgtcatggt tctttatccg
     2581 acggcgggtt tgcacgtgag cgcgctgcac gaaaaagatt acgcgacggc aatcacgcgc
     2641 gcctacaacg a
//
LOCUS       DASUTG010000812         8365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1160942, whole genome shotgun sequence.
ACCESSION   DASUTG010000812 DASUTG010000000
VERSION     DASUTG010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160942"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..489
                     /locus_tag="VF089_18840"
     CDS             94..489
                     /locus_tag="VF089_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176075.1"
                     /translation="MKGYVTNIEDDTMKNKDFRRVLYTGKNSQLVLMSLRPDEEIGLE
                     THTLDQFIRVEAGSGIAVLDGVEHQIADGTAVVIPAGTKHNVINNSEREELKLYTLYS
                     PPEHRDGTIHKTKADALAHEEHFDGKTTE"
     gene            852..1304
                     /locus_tag="VF089_18845"
     CDS             852..1304
                     /locus_tag="VF089_18845"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX6176076.1"
                     /translation="MDAEVYPDPRVENFINANYLPVKIHIKEQPQIFERFGAQWTPTI
                     QVLDPDGTKRHQFEGYLPPDDFLAQLQLGVAHSAFARQQWKDAEAAYNQILNTLPESD
                     AAPEALYWRGVSQYKSSGDPTALQQTAEAFKTRYAGSTWAKKASVWAK"
     gene            1669..3054
                     /locus_tag="VF089_18850"
     CDS             1669..3054
                     /locus_tag="VF089_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEX6176077.1"
                     /translation="MTINYDAVIIGAGQAGPPLAVRLASAGKKVAIVERNQFGGTCVN
                     TGCIPTKTLIASARVAHMARRAMEYGVGIGSSITVDMKRVKERKDEVVRRSNEGVEKW
                     LRSTDNVTVYQGHARLEDRHRVRVGSELIEADQIFINVGARANVPQLRGIDQVHYLTN
                     SSMMDVDYLPEHLIIVGGSYVGLEFAQMFRRFGSEVTVVEMGPRLIHREDEDVSEAIR
                     EILQNESIHIRLNAECIALEKQGDKVAVDLDCSTGDKQVIGSHLLLAVGRVPNTDDLG
                     LEKAGVVVDQRGYVQVDDQLRTNVPGIYALGECNGRGAFTHTSYNDYEIVAANLLDGD
                     RRRVSDRINAYALYTDPPLGRAGMTEAQVRKSGRKALLATRPMTRVGRAVEKGETQGF
                     MKILVDAETKEILGASLLGIECDEVIHSILDVMYAKAPYTVIQRAVHIHPTITELIPT
                     LLGDLKPLDPT"
     gene            3748..4416
                     /locus_tag="VF089_18855"
     CDS             3748..4416
                     /locus_tag="VF089_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX6176078.1"
                     /translation="MGPINRPDQLSEEMIDATLADSFPASDPPSWTLGRDRNEQEEIA
                     GAAVPPTSKIHEKEKFIMKTNIDLSEEQRAGVVKILNTLLSDEYLLYTKTRNYHWNVT
                     GPQFNDLHKFFEEQYTQLSEIVDQVAERGRTLDGWAFGTMSEFSQHARLKEHPGHYPG
                     AREMIANLVDDHETVIRQLRSDLETCADKYHDMGTNDFLTGLMEQHEKMAWMLRAFLQ
                     AESV"
     gene            4514..5137
                     /locus_tag="VF089_18860"
     CDS             4514..5137
                     /locus_tag="VF089_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX6176079.1"
                     /translation="MATNEAYQAKQFKLSGLQGISDQTLEMHFKLYEGYVKETNRLTE
                     KISELLQDGQVDQEEMPAYSELTRRMGFEYNGMVLHEYYFGNMIKGGSGDPGKQSAFY
                     KAAETGFGSYDIWKADFVSIGKMRGVGWAICFQDPKRRRLSNHWVSLHEIGNVAGFEP
                     VLVMDVWEHAFLLDYKPAERPKYIEAFFANIDWNAVEQRLQGDFKKT"
     gene            5872..6159
                     /locus_tag="VF089_18865"
                     /pseudo
     CDS             5872..6159
                     /locus_tag="VF089_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            6417..6710
                     /locus_tag="VF089_18870"
                     /pseudo
     CDS             6417..6710
                     /locus_tag="VF089_18870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
     gene            6706..7269
                     /locus_tag="VF089_18875"
     CDS             6706..7269
                     /locus_tag="VF089_18875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX6176080.1"
                     /translation="MPPSSSIYGDGYGAPPKRLERCLSTEYAEQVVRLSLVSQVAQSY
                     FDLRNLDLQLDIARGRLASREEALRLVTKRFEGGVVSELDLRQAESEPAVAAAAVPNL
                     EQQIVQRENELSILLGRNPEDIMRGRGVFESAIPEVPVGLPSTLLVRRPDILAAEESL
                     VAANAQAGAARAASAPLLDRLTRGGEH"
     gene            7256..7453
                     /locus_tag="VF089_18880"
     CDS             7256..7453
                     /locus_tag="VF089_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176081.1"
                     /translation="MESTELSDWFSRGSRVWQMTGGLTAPIFTAGRIGAEIGTATAQQ
                     QQALFDYLRTIQTGLREVEDS"
     gene            7450..7680
                     /locus_tag="VF089_18885"
     CDS             7450..7680
                     /locus_tag="VF089_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176082.1"
                     /translation="MIATRKIREQQAAQDRQVQVLKEPCGLPRYAMRTAIRIFWKSST
                     QRSLFSAELQQVQLQRARLNAIVNLYKALGGG"
     gene            complement(7711..>8365)
                     /locus_tag="VF089_18890"
     CDS             complement(7711..>8365)
                     /locus_tag="VF089_18890"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX6176083.1"
                     /translation="FEDAGAAYVDVARRPRGIGAMSKFLESRSVKRPPFAPPFLKAGK
                     LLIAQTANILFFLGPRLGLAPKDEAARLWAHQLQLTIADWVAEVHDTHHPVASSLYYE
                     EQKRESKRRAEIFRVERQPKFLTYFEQILERNGGRVLIGNTISYVDLSLFQMIEGLRY
                     AFPRAMGKEERKYPRIIALHDRTVERPRLAAYLASERRLPFNQDGIFRQYAELDDRS"
BASE COUNT         2028 a         2171 c         2372 g         1794 t
ORIGIN      
        1 atctacattt cttcactgcg cacggctgag aaaacatgag gaagaatccg ccatcaggta
       61 aagagtcaca cccaataata ggagggaaca gatatgaaag gttatgtgac aaacattgaa
      121 gacgatacga tgaaaaacaa ggatttcagg cgagtgttat acacaggaaa aaattcacag
      181 ctggttttaa tgagccttcg gccagacgag gagattggct tggaaactca taccctcgac
      241 cagttcatcc gagtcgaagc gggtagcggt atcgcggttc tcgatggagt agagcatcag
      301 attgcggatg ggacggctgt ggtgattcca gcgggcacga aacacaacgt catcaataat
      361 tcagagcgag aggagttaaa gctctatacg ctttattctc caccggagca ccgcgacgga
      421 acaatccata aaaccaaagc cgatgcgctt gcgcacgagg aacacttcga cggtaagacg
      481 accgaatagc tgattatggt cgtcacgacc ggcgaatata tgaatggcac agaacccgaa
      541 gctcgttaag gataatgatt cgcaggttct tttcgcctcc gtcctactag gtcggtatgt
      601 tccacagctt gcgcgaaatc ttccgacgct catccggctg tcgggccctc gggtttgaag
      661 gcgtactcga tcattgcgcg ttagatgaaa tggcttaggc atggataacg aggcgattag
      721 tcaacaggag gaagcttatg gcagtcgact ggaaaaggga tattgatgct gcgcttgtgg
      781 aagcacaatc cagcaggcgc ccgttgttgg tcgattttaa cgcgactccg atgtgaggag
      841 cttgcgctcg gttggatgcc gaggtttacc cggacccgcg agtggaaaat tttattaatg
      901 ccaactatct ccctgtaaag atccacatca aagagcagcc acaaatattc gagcgcttcg
      961 gcgcgcagtg gacgccaacg atccaagtgc tcgatcctga tggtaccaag cgccatcaat
     1021 tcgagggtta tctgccgcca gacgactttc ttgcgcaact gcaactcggc gtcgcgcatt
     1081 cggcctttgc acgtcagcaa tggaaagacg cggaggccgc ctacaatcag atcttgaaca
     1141 cactgccgga atccgatgcc gctccggaag cgctctactg gcgcggcgtt tctcagtaca
     1201 agagcagcgg tgatccaacc gcactgcagc agacggcaga agcattcaag actcgctatg
     1261 cgggcagcac ttgggcgaaa aaagcttccg tatgggcaaa ataacgacaa ggtcaatgaa
     1321 aaaaatcaag ggatcaatga aaagatgatt aaccggtact cgtgagatga tcaaagcgac
     1381 ttgcgttgct gagaataagg ccgagcggaa ctcacgggat tagcaaaact aagcataaaa
     1441 cggatatggc tatcccgtgc cgcatgaggc ccaatgtaaa gttgattcgg cggagctgtc
     1501 gttgaatcaa ggagagttaa tttctgtcga caaggcggcc atcattgcaa ccggccgaga
     1561 tcttgacggt gggtgttgcg gtgattgcac tatgcgacac atcgattggt tagggcgcag
     1621 catgctgcgt ttctagcgct acatgaccag tcgccgggag aaatcaagat gacaattaat
     1681 tatgacgcgg taatcatcgg cgcggggcaa gcggggccac cgctggcggt ccggctggcc
     1741 agcgccggaa agaaagtggc gattgtcgag cgcaaccagt ttggcggcac ctgtgtcaat
     1801 accggctgca tcccgaccaa gactttgatc gccagcgccc gggtggcgca catggcgcgc
     1861 cgcgcaatgg aatatggcgt cgggatcggt agctcgatca ctgttgatat gaagcgagtg
     1921 aaagagcgca aagacgaggt agtgcgtcgc tccaacgaag gtgtcgagaa atggctgagg
     1981 agcaccgaca acgtaaccgt ttaccaaggt catgcccgtc ttgaagaccg gcatcgcgtt
     2041 agggtcggca gtgagctgat cgaagcagac cagattttca tcaatgtcgg cgcgcgcgcc
     2101 aatgttcccc agctccgcgg catcgatcag gtacactatc tcaccaactc gagcatgatg
     2161 gacgtggatt acttgcccga gcatctgatt atcgttggcg gcagctatgt gggtttggaa
     2221 ttcgctcaaa tgtttcgccg ctttggcagc gaagttaccg tcgtcgaaat gggaccgcgg
     2281 ctgattcatc gtgaagacga agacgtttcc gaagccatca gggagatcct gcaaaacgaa
     2341 agcatccata tccggcttaa tgccgaatgc attgccttag aaaaacaagg cgacaaggtc
     2401 gctgtcgatc tcgattgcag cacgggagat aagcaagtca tcggctcgca tttgcttctc
     2461 gcggtcggcc gcgtgcctaa taccgacgat ctcggtttgg aaaaagctgg cgtcgtcgtc
     2521 gatcagcggg gctatgttca ggtagacgat cagttacgga cgaacgttcc cggcatctac
     2581 gcgctgggcg aatgcaacgg ccggggagct ttcacacaca cctcatacaa cgattatgag
     2641 atcgtcgccg ccaacctcct ggacggtgat cggcggcgcg taagcgatcg catcaacgcc
     2701 tatgcgcttt atacagatcc gccgctcggg cgcgccggca tgactgaggc gcaagtgcga
     2761 aagtctgggc gcaaagcttt acttgcgaca cggccgatga ctcgggtcgg ccgagcggtg
     2821 gagaaaggcg agacgcaagg attcatgaag atcctggtgg acgcggagac caaagaaatt
     2881 ctcggagcat cgctgctggg catcgaatgc gatgaagtga ttcattcgat tctcgacgtg
     2941 atgtatgcca aagcgcctta tacggtcatc cagcgcgccg tgcacatcca tcctaccatt
     3001 acggaattga tcccgacttt gctgggcgat ctcaaacctt tggaccctac ctagtagaaa
     3061 caacaaggtt ggtgatagca attctgcctt ctaaccaagt ttggcgaaac cgatcggaca
     3121 cgtttgacac ggggccgatg cttcaggcgg atgctcatcg aaaaggatca gagtgcctcg
     3181 gtattggtca gcaatcacag cgtatcaagc gtgtacgggc cgaccaattt ggagtaaatc
     3241 attatccgtt ctactcgatc gaaagactcc cacgctgtgt taatactttg atggtcattc
     3301 gccgagtact ggagtcgccg atctgaccat tgggtaaagc gaagcgcacc tagctaactc
     3361 gtcaccactt tatctcgttc agttcttcca ggtgacttta gatcgtagcc tgtgccagct
     3421 gtgcgagatt tggaagacgt agaactgttt agaaacggga attatctatt cgaactgcgg
     3481 taagtcgcag ttgcgacaaa gaagtgacga gtcatctgcg gagaggaaac agaaaatttc
     3541 tgaggcttac tacttgatcg gttcggtcat agttatagcc tgaatcagga tacctctgta
     3601 gccggcgcca agtattaggt tctggagaaa ctgcatcaac aatagcaacc tgaaactgtt
     3661 ctggcacatg gctcgaaaaa taaattttac cgggtcttaa gtaactatga atagctaaac
     3721 agttagttag attaaaagga gcacgccatg ggacccatca atcgacccga tcagctctct
     3781 gaagagatga tcgatgcgac tttggcggac agctttcccg caagcgatcc gccgtcatgg
     3841 acactaggcc gtgatcgtaa cgagcaagaa gaaattgccg gtgccgcagt ccccccaacg
     3901 tcaaaaatac atgagaagga gaaatttatc atgaaaacca atatcgacct ttcggaggaa
     3961 caacgcgccg gggttgtgaa gatcctcaac accctgttgt ctgatgaata tcttctctac
     4021 accaaaacac gaaactatca ttggaacgtc accgggccgc agttcaacga tctgcacaag
     4081 ttttttgagg agcaatatac ccagctcagt gaaatcgttg atcaggttgc ggaacgcggg
     4141 cgcactcttg acggttgggc attcggcacc atgagcgaat tctcccagca cgctaggctc
     4201 aaggaacacc cgggccacta tcccggggcg cgcgagatga ttgccaacct tgtagacgac
     4261 catgagacag tcatccggca attgcgcagc gatctggaga cctgtgccga caaatatcat
     4321 gacatgggca ccaacgattt tcttacaggc ttgatggaac agcatgaaaa gatggcctgg
     4381 atgctaagag catttctgca agccgagtca gtctagttca ggttcttagc atttggatca
     4441 gctttcactg attgccgggg caacttcagg ctatacaggc aaagaatttt aagacaaagg
     4501 aaggggatta tccatggcga caaatgaggc gtatcaagcc aagcagttca aactgagcgg
     4561 ccttcaaggg atttccgatc aaacgctcga gatgcacttc aagctctatg agggttacgt
     4621 caaagaaacc aaccggctga cagaaaagat ttcggagttg ttacaagatg gccaggtcga
     4681 ccaggaggag atgccggctt attcagagct cacccgccgc atgggtttcg aatacaacgg
     4741 catggtgctg catgagtact atttcggcaa catgatcaaa ggtggatcgg gtgatcctgg
     4801 caagcagtcg gctttttata aagctgcaga aaccggcttt ggcagctatg atatttggaa
     4861 agccgacttt gtcagcatcg gcaaaatgcg cggcgtcggc tgggcgatct gctttcagga
     4921 tccgaaacgc cgccggctga gcaatcactg ggtaagcttg catgagatcg gcaacgttgc
     4981 gggattcgag ccggtgctgg tgatggacgt atgggaacac gcgttcctgc tcgattataa
     5041 gcccgctgaa cggcccaaat acatcgaagc gttttttgcg aacatcgact ggaacgcagt
     5101 cgaacagcgt ctccaaggcg atttcaagaa gacatagatc cgatgacgat atccttcgga
     5161 atatttcatg gtggtgcttc gcgaccgtct tggtcgcgaa actattccga tgatacagct
     5221 gctatgatct agcgctccgc attcgtgcag gcgcgccgca ttgcttggcc gatgggctgt
     5281 cccattagtc tacgcttagc gagcaatgcg gcgcgcgctg ctgcggtgat tcagacaaac
     5341 aactcgagtt cgacgggaga gactcgatgg aagccacatc cgcggatgcc tcaatatcat
     5401 ctccccggct cctgcgattg gtttagcgat tttcatttca cttactctcg ccctcgacgg
     5461 ctgtgatcga aacggccaga gtgaaaattc aaaaactgcc aagaaatccg aagctgcccc
     5521 ggccgaagtc agagcgatca ccaccgtacc gaaaaacttt gccggttacc tttgaagcgg
     5581 tcgggcaaac cgagggctct cgcgaagtgg aagtacgagc tcgagagggc ggaattctgc
     5641 tgcgccgtct ttataccgag ggtagctggg tgaagcgggg gactctcttg ttcaagatcg
     5701 attcgcggca aagccgcggt gaagaagcta agggagaaat cgaaaggata gcctggtccc
     5761 ggtgggacgg acttgctgag cacaatgtca caggtccatc ccatctatgc tatctatgct
     5821 aatttcagct actctgaaat tgacatgctg cgcgtgcggg acgaaccgta tgatgcagcc
     5881 ccgtgcgcag cgcgactgcg cttccgtccg atcatcgtga catcgctggc gttcattcta
     5941 ggtgtcgtcc cgttggtctt gagtaccggg gcgggggcgg caagccggca ctcaatcggc
     6001 accggcgtga tcggaggcat gttagccgcc accctcgtag cgactttgtt tgttccgttt
     6061 ttctacgttt tgttgaccaa acgcagcgat cccaaaccgg ctgaagagcc ggagcccagc
     6121 ccagcggaag cagaagcagt taaaggtagt ggatcatgag gaggcttgcc gcactcttat
     6181 ccaaaataga tcaagtcctt ggggcggtgc taattacttt ccccctttgg aaaaggggga
     6241 ttgaggggga tttctttcgg tcgactgcga ggcaagtctt tcggaaaaat cttcccttac
     6301 ccctcttttc caaagagggg aattggtaac ggcgcagttc tcctcagcgt tcttgggatc
     6361 tccggctgta tgatcggcgc cgattacagg cggcctgatg taaccgtccc tcccggatgg
     6421 cgcacgatcg atggggatgc aaccgctgtc atcaacgaac agtggtggag gcattcaatg
     6481 atccacaact cgaccggctg attgagatcg cgttggcgga aaatctggat gtcgccagcg
     6541 ccgtcgctcg ggtcgaagag gcgcgagcgc tattagcgac gtcgcgagcg gctctcgtcc
     6601 cccaagtcgg cgcctcggcg agcgcagcgc gctcgagaag cagtaccggg acaactccgc
     6661 ttccaggagt gagcctcacc aacaattttt attcagctgt tctggatgcc tccttcgagc
     6721 tcgatctatg gggacggcta cggcgcgcca ccgaagcggc tcgagcggtg cttgtctaca
     6781 gaatacgccg agcaagtcgt tcggctctcg ctggtgagcc aggtggcaca gtcatatttc
     6841 gacctgcgca acctcgatct acagctcgat attgcgcgag ggagattggc gtcgcgcgag
     6901 gaagctttgc ggttggttac caaacgtttc gaaggcggcg tcgtttccga gctggatctg
     6961 cgtcaagccg aaagcgaacc cgcggttgcc gctgccgcgg tgcccaatct cgaacagcaa
     7021 atcgtccaga gagaaaatga gctgagtatt ctgctcggcc gcaatcccga agacatcatg
     7081 cgcggtcgcg gcgtgtttga atccgcgatt cctgaggtgc cggttggcct gccttccact
     7141 ctgctcgtcc gtcgtccgga tattctggcc gcggaagaaa gcctggttgc cgccaatgcg
     7201 caagctgggg cggccagagc agcttccgcg cctcttcttg accggcttac tcggggtgga
     7261 gagcactgag ctgtcggact ggttcagccg cggctcgcgc gtatggcaga tgaccggagg
     7321 cctgacagca ccgatcttta cggcgggaag aattggcgcc gagatcggga ccgccaccgc
     7381 gcagcagcaa caggcgctat tcgactatct gcggaccata caaacgggct tgagagaagt
     7441 ggaagattct tgattgcaac ccgaaagatc cgggaacaac aggcagcgca agatcggcag
     7501 gttcaggtgc tcaaagaacc ttgcggcttg ccacgttacg ctatgagaac ggctattcga
     7561 attttctgga agtcctcgac gcaacgcagt ctgttcagtg ccgagctcca gcaggtacag
     7621 ctccagcgcg cgcgattgaa cgccatcgtc aatctctata aagctctcgg cggtggatag
     7681 gtgccataat aacgttctgt tcgcatccga tcacgaccga tcatcgagct cagcgtattg
     7741 gcggaaaatg ccgtcctggt tgaacggcag acgccgctcg gatgcgagat acgccgccag
     7801 ccgcgggcgc tccaccgtcc ggtcatgcag cgcaatgatt cgtggatatt tgcgttcctc
     7861 ttttcccatg gcccgtggaa atgcatagcg caacccttca atcatctgga agagcgacag
     7921 gtcgacgtag gagattgtat tgccgatgag aacccggcct ccgttgcgct ccaaaatctg
     7981 ctcgaagtaa gtcagaaact tcggctggcg ttcgaccctg aaaatctccg cgcgccgctt
     8041 cgactcgcgc ttctgctctt cataatacaa gctgctggcg accgggtgat gcgtgtcgtg
     8101 aacctcggcg acccaatcgg cgatggtgag ctgtagttga tgcgcccaaa ggcgcgccgc
     8161 ctcgtctttt ggcgcgagcc caagacgcgg gccgaggaag aacaggatgt tggcggtctg
     8221 ggcgatgagc agtttgccgg cttttaaaaa tggcggcgcg aacggtggtc gcttgaccga
     8281 ccggctttcc agaaacttcg acattgcccc aatcccgcgt ggccgccgtg cgacatcgac
     8341 ataagccgcg ccggcgtctt cgaag
//
LOCUS       DASUTG010000813         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1161016, whole genome shotgun sequence.
ACCESSION   DASUTG010000813 DASUTG010000000
VERSION     DASUTG010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161016"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VF089_18895"
     CDS             <1..1343
                     /locus_tag="VF089_18895"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX6176084.1"
                     /translation="ELDATSPEDVARVTKPVSPRYEMSAILTHLERHKRFPLLFFQNS
                     KDSQAPVVINAQASRRLMSIALECKPEDLARAFSERQSKPLAPVEMINAPVHEIARTG
                     DEIDLNEVPLLTHYDVNIAPYITAGIVVAVDPDTGVRNTSYNRLMMAGNRELRIFMAV
                     GRHLWTLHNKLERRNQPLPIAIIIGVHPLFSLGAQALTPADEDEYAVIGGMMNEPLRV
                     VRAKTVPILVPADAEMIIEGQIMPNLRRTEGPFGEFTGHAVPQDQRQVIEITAITHRE
                     NYIFQDIHAGYTEHKLMGAVPREAALLKAVRLAVPTVTNVCMPVSGNCRFHAYISIAK
                     RTPGQAKNAVCAAVAADMLLKHVVVVDDDIDVFDEERVLWAISNRFQADRGLVVIANA
                     QGSELDPSAGPGGINAKMGLDATKPLSGFPPELRVPEEVLEKITLDDFLPDFAK"
     gene            1678..2544
                     /locus_tag="VF089_18900"
     CDS             1678..2544
                     /locus_tag="VF089_18900"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX6176085.1"
                     /translation="MLAVLAFFWQPSPVISTKPAAKPEHSKPDIEQARAMAQPAAPEV
                     QAPAPHPLDRTVIPLGVKRIVIDAGHGGKQPGAISESGVSEKDLTLDIALRLHRLLNQ
                     APFEVLMTRQDDRTMTLEKRVAFANSNRADLFISIHINWTEPREIRPLETYFVGPSDD
                     AATMKLASMENQESGYSLSEYRRVLEKIYIDTRRDESRSLAKNIHAELYHSLKAVNPT
                     LENRGVRTAPFLVLVGTEMPAILLEVSTLSNKEEVELLIDPDYREKIALAVSRGIGSY
                     AKNLNSHRSAER"
     gene            2555..3586
                     /locus_tag="VF089_18905"
     CDS             2555..3586
                     /locus_tag="VF089_18905"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6176086.1"
                     /translation="MEETNETLCVGIDLGTSRSSISASNGERHVIDSYVGWPLDMVAR
                     KLIQKPILVGREALDSRSMLDLRRPMERGLIKDGSKKDEEAVQEILRHLITQTGLKQK
                     NKTRSKVRAVVGVPAEAFRNSKQRLRSAMEGVADSVMLVSEPFAVAYGLEALLHALII
                     DIGAGTADFCVMQGRYPTEEDQRTLPNAGDSIDEQLLKLVREHHPEAQTSIHMIREWK
                     EKWSFVGEAKQPVIVSVPVKGKATELDITAEMRAACESILAPVCETMLDLLTRVEPEY
                     QAKVRNNVILAGGTSLIDGLSQAIEKALAELGGGHVTVVKDPVFAGSDGGLAIARDAS
                     SSDWEKLSL"
     gene            complement(3696..>4396)
                     /locus_tag="VF089_18910"
     CDS             complement(3696..>4396)
                     /locus_tag="VF089_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6176087.1"
                     /translation="IGEDVSKLFAHEGAKVAVVDLDQGRGSRVASEINATKPGSAISV
                     VCDVASADSVDQMVQTVVKQFGAIDILVNNAASTDHKTLFDITEEEWDRVMNVCLRSV
                     WYCTRSAAKVMIDHKRKGKIINIASTSGHWGRKEATAYTTAKAGVLNLTRSIAVQLAP
                     EGIRVNSVSPNRIGSPVGQEEVPENRFVKNLAGRRGVPMDIAKAVVFLASDESDFIYA
                     IDLPVDGGALAIRD"
BASE COUNT         1052 a         1193 c         1171 g          980 t
ORIGIN      
        1 ccgaactaga cgctacttca cccgaagatg tagcgcgggt taccaaaccg gtctcgccgc
       61 gctacgagat gtccgcgatt ctcacccatt tggaaaggca taaacgcttt ccgctgctgt
      121 tctttcagaa ttcgaaagat agccaggcgc cggtagtcat caacgcccag gcgagtcgcc
      181 ggctcatgtc catagcattg gaatgtaaac cggaagactt agcgcgcgca tttagtgagc
      241 gccaatctaa acctttagcc cctgtggaga tgatcaacgc gccggtgcac gaaatcgcca
      301 ggaccggcga tgaaatcgat ttgaacgaag tgccgctgct gacccactat gacgtcaata
      361 tcgcccctta cattaccgcc ggtatcgtcg tcgcggtcga ccccgacacc ggcgtgcgta
      421 atacgagtta caaccggctg atgatggcag ggaaccgcga gttgagaatc ttcatggctg
      481 tcggcagaca tctctggaca ctgcacaaca agcttgagcg acgcaaccaa ccgctgccca
      541 tcgccatcat cattggtgtt caccctcttt tttctcttgg agcacaagcg ctgaccccgg
      601 ccgatgaaga cgagtacgcc gtcatcggcg gcatgatgaa cgaaccgctt cgtgtggtac
      661 gagcgaagac agttccgatt ctcgtaccgg cggatgctga gatgattata gaaggccaga
      721 tcatgccgaa tctgcgacgg accgaggggc catttggcga gtttaccggt catgccgttc
      781 ctcaggatca gcggcaagtc atcgaaatca ccgctatcac ccaccgcgag aattacattt
      841 ttcaggacat tcatgccggg tatacggagc acaaactaat gggcgcggtg ccgcgcgaag
      901 ctgctctgtt aaaagcggtt cgcctggctg ttcctaccgt cacgaatgtc tgcatgccgg
      961 tatcgggcaa ctgccgcttt cacgcttaca tttcgatcgc taaacgaact ccgggtcagg
     1021 ccaaaaacgc ggtctgcgcg gccgtcgccg ccgatatgct gcttaagcat gtggtggtcg
     1081 tcgatgacga catcgacgtc ttcgacgagg aaagggtgct gtgggcaatc tcaaatcgct
     1141 ttcaagcgga ccgcgggctc gtggtcattg caaatgctca aggcagcgag ctcgatccct
     1201 cggctggccc cggcggtatt aacgcaaaga tgggcctgga cgcaactaaa ccgctgagtg
     1261 gctttccgcc ggaactcaga gtgccggaag aagttttgga aaagattacc ttggacgact
     1321 tccttcctga ttttgcaaaa taatacctga cgctttgctc gatcgttatc cactaacgcc
     1381 cagcattcgg ctgccgtaag tttatcttac gaccgttgct catctgggat gtgcccatca
     1441 ccatagtacc ggtagcattt gacagggcaa ttcatcatcg gctaaccttg cacatcttcg
     1501 atcctttctt tgaatgatca gtcaagctac ctcattcgaa tggcgagcat agagcaggac
     1561 aaactccgcc tccttagggg cgcagtccaa gacaacgtcg acctgctgcg cggaatccgg
     1621 acccatactc ggaagcggca tcgcaaagtc acacgaccgt ggatatggag tcccttgttg
     1681 cttgccgttc tcgcattttt ttggcagcct tcgccggtca tttcgaccaa gcccgctgcc
     1741 aagccggaac attcgaagcc ggacatcgaa caggctcgag cgatggcgca acccgctgca
     1801 ccggaggttc aggcgccagc gccgcatccg ctcgatcgca cggttattcc gctcggggtt
     1861 aagagaatcg tcatcgatgc cggtcacggc ggcaaacagc cgggagcgat atccgaatcc
     1921 ggggtctccg agaaggacct taccttggac attgcgctac gtttgcaccg cctccttaac
     1981 caggcgccat tcgaagtgtt gatgacccgc caagatgacc gaaccatgac gctggagaaa
     2041 agagtggcgt tcgccaattc gaacagagcg gatctattta tttcaattca tatcaactgg
     2101 accgaaccgc gcgaaatccg ccccttggaa acctacttcg ttggccccag tgatgatgcg
     2161 gcaacgatga aattggcgag catggaaaac caggaatccg gctactcatt gtcggagtat
     2221 cgacgggtat tggaaaaaat ctatatcgat acgcggcgcg atgaatcccg cagtctggcc
     2281 aagaacatcc atgcagaact gtaccattcg ctcaaagcag tgaatccgac cctggagaat
     2341 cgtggtgtta gaacggcgcc attcttagtt ctggtcggca cggagatgcc ggcgatactc
     2401 ctcgaagtgt ccacactatc aaacaaagaa gaagtagagt tgttgataga ccccgattac
     2461 cgagaaaaaa tcgcgctggc cgtatccaga gggattggca gctatgcaaa aaatcttaat
     2521 agtcataggt cagctgaaag gtagctaatt gtctatggaa gaaacaaatg aaactctttg
     2581 cgttggaatc gacctcggca cgtcgcgaag ttctatttcg gcatccaatg gcgagcgcca
     2641 tgtaatcgac agttatgtcg gttggccgct cgatatggtc gcgcgcaagc tgatccaaaa
     2701 accgattctt gtcggtcgtg aagcattgga tagccgttcg atgctggatc tgcgccggcc
     2761 catggagcgc ggattgatta aggatggatc aaaaaaggac gaagaagccg ttcaggagat
     2821 cctccgccat ttgatcacgc agactggttt gaaacagaaa aataaaacac gttcaaaggt
     2881 tcgcgccgtg gtcggcgtgc ccgccgaagc atttagaaat agcaaacagc gattgcgcag
     2941 cgccatggaa ggggtcgccg atagcgtcat gttggtgtcc gaaccatttg ctgtcgccta
     3001 cggattggaa gctttactgc acgcgctgat catcgatatt ggcgcgggga cagctgactt
     3061 ttgcgtgatg caagggcgtt atccgactga agaagaccaa agaaccttgc ccaatgccgg
     3121 cgattcgatt gacgaacaac tactgaaatt agtgcgcgag caccatcccg aagcgcaaac
     3181 gtcaattcat atgattcgtg aatggaagga gaaatggagt tttgtcggtg aagccaaaca
     3241 accggttatc gtttctgttc cggtcaaagg aaaggcgacc gagctggata tcaccgcgga
     3301 gatgcgcgcc gcctgcgaaa gcattttggc gccggtttgt gaaactatgt tggatctgct
     3361 caccagagtc gagcccgaat accaagcgaa ggtccgcaat aacgttattc tcgccggcgg
     3421 cacttcgctc attgatggac taagccaggc aatcgaaaag gcgcttgccg agctcggtgg
     3481 cggccacgtc acagtcgtta aggatccggt ttttgcgggg tctgatggcg gcctggcgat
     3541 tgctcgggat gcctcgagct cggattggga gaagttgtca ctgtagctga tctaactctc
     3601 cgttcccgaa aatctgaatg catcacctgc cgatgaattt agctcttctc aaatcttcac
     3661 gttctaagct tcaggcaaga tgtagcaacc aaaagttaat cgcgaattgc tagggcgccg
     3721 ccgtctacag gcaggtcgat tgcgtagatg aaatcggact catccgaagc cagaaagact
     3781 acggctttgg cgatatccat tggaactccg cggcgccctg ccaagttttt gacgaaacga
     3841 ttttccggaa cttcttcctg accaactggc gagccgattc gattcggcga taccgaattc
     3901 acgcgaatgc cttcgggcgc taattggact gcaatcgatc gggtgaggtt aagcacaccc
     3961 gctttggccg tcgtgtaggc tgtggcttct ttacgtcccc aatgccctga ggtcgaagcg
     4021 atgttgatga tcttgccttt gcgcttgtga tcgatcatca ccttcgcagc cgatcgcgta
     4081 cagtaccaca cgctcctgag gcacacgttc atgacacggt cccactcttc ttcggtaata
     4141 tcgaacaagg tcttgtgatc ggtcgaagcg gcgttgttga ccaagatgtc gatagcgcca
     4201 aattgcttca ctaccgtctg aaccatttga tctaccgaat cagccgatgc gacatcgcat
     4261 acgaccgaga tagccgaccc tggcttggtc gcgttgattt cactagccac acggctgcct
     4321 cttccctgat cgagatcgac gaccgccacc ttggcgcctt catgagcaaa aagcttggag
     4381 acgtcttcac cgatat
//
LOCUS       DASUTG010000814         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1161168, whole genome shotgun sequence.
ACCESSION   DASUTG010000814 DASUTG010000000
VERSION     DASUTG010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161168"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2968)
                     /locus_tag="VF089_18915"
     CDS             complement(<1..2968)
                     /locus_tag="VF089_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6176088.1"
                     /translation="MIADRLDSKKIVFELKGTLVKAIDELCARSSVADLAGHFRAQKS
                     DENERYSCIGHGIAVPHVRIDHLAAPELILGISPEGLSFANHRIHIVLLLATPAEQPP
                     QHLQILQRICSLLPAIRDELLAQRDAVRVLRIIARAEQQSALPTYLNLTQEQIGFELQ
                     TNLAHGLTSTEAQQRLHHYGPNLLQRSRVDPWQLKLLRNLFSFFAILLWIAALLCFIP
                     GVDLPQLGIAILAVILVNGLFAFLQEYKSDRSLEILQQLITQRCQVMRDGNLQECDAS
                     QLVPGDVIVVEEGDLVPADARLLEAFEVELDNSSLTGEATPARRYKSDQPILIPGKFL
                     WLELPNVIFAGTSLLRGRARAVIFGTGMNTEIGKIANLTRDIRAEPSPLQKQLRATVY
                     SIAALAGGLGLAFLMLGWLVAGLTFLAAFVFCIGLFVANVPEGLLPTVTLSLAMGVQR
                     MAKRHALMKNLSSVETLGCTTVICCDKTGTLTQNLMMATEIAVDGKIVQVSGAGYRPQ
                     GEFNTSGKRLSLDAISHWPTLTRLLDCAFTCNNARLSKQGADYAVIGDPTEGALVCLA
                     EKAGLRGVHQRLHLNPFESIRKRMSVVIKAHQQSETVIYVKGAPLETLQQCDRFSWNG
                     EIRPLTDAERRRILEENDAMAQRGLRVLAFAYREGAELNGAAYTTAYIETHLVFLGLV
                     ALSDPVRAEVPGAIRDCHTAGIRVLMVTGDYPLTAASIGRQIGLGQNGSLKCFTGAEV
                     SELDDNALREILSQQETIFARVAPEHKLRIVSLLKQMGEIVAVTGDGVNDAPALKKAD
                     IGIAMGMRGNDVAKEASRMILTDDNFSSIVAAIEEGRAIFDNIKRFAAYVLNSNPQEM
                     YPYIFWVLFPGTPLAMTIMGVLAVDVGTDLIPAMGLGIEPPEKGIMERPPRPRNEKLL
                     SLKFILRSYFVQGTLLALSCYATYFYMGWVLGAWQLGQSIASMPPSPAGLNFGEASSA
                     YLQTLTAYFFPTVTAQI"
     gene            3100..3267
                     /locus_tag="VF089_18920"
     CDS             3100..3267
                     /locus_tag="VF089_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176089.1"
                     /translation="MADDHQARDQGQVFGELYEKGADHAPGREKRLRASRLALLPMEH
                     VGILAREHATG"
     gene            complement(3268..3696)
                     /locus_tag="VF089_18925"
     CDS             complement(3268..3696)
                     /locus_tag="VF089_18925"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6176090.1"
                     /translation="MKTKNEKKGTVKEIMMGSPVTLAPNDTLDLANDVISLGRIRHIP
                     ILDNGKLVGLISGRDLIGAAANRIFGLKQKSRSALLKGELIKNIMKKRVITVTLDTPI
                     KDAAMLMANKKIGCVPVLSDGTLVGLVTTTNVLHYLGSIA"
     gene            complement(4693..5082)
                     /locus_tag="VF089_18930"
     CDS             complement(4693..5082)
                     /locus_tag="VF089_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6176091.1"
                     /translation="MFAKDVMTRDIITISPTTKVRNLAMILIKNQISGAPVVDEHGKI
                     LGIVSEADIVAKKGKDAKSIMSKKVVTVREDAPVEEIAQLMITHAIKRVPVTDGGKVI
                     GIVSRADIISAIAMGEHVAIRTPIYDL"
BASE COUNT         1188 a         1339 c         1405 g         1178 t
ORIGIN      
        1 ctatctgcgc ggttacagtg ggaaaaaaat atgcggtcaa tgtttgcaga taagccgacg
       61 acgcttcacc gaagttgagg ccggcgggtg acggcggcat cgaggcaata ctctggccca
      121 gttgccacgc gcctagcacc caacccatat aaaagtaagt agcgtagcag ctaagcgcga
      181 gcagagttcc ctggacgaaa taagaacgga gtatgaactt gagcgaaagc agcttttcgt
      241 tgcgcggcct tggcggtcgc tccatgatac ccttttccgg cggctcgatg cctaagccca
      301 tcgccggaat gaggtccgtg cctacgtcga ccgccaaaac gcccatgatt gtcattgcga
      361 gcggagtgcc gggaaaaagc acccaaaaaa tgtacgggta catttcctgt gggttactgt
      421 tgagcacgta ggcggcgaat cgtttgatgt tgtcgaatat cgcgcggccc tcttcgatgg
      481 cggcgacaat ggagctgaag ttatcgtcgg tcaaaatcat gcgcgaggcc tctttggcga
      541 catcgttacc gcgcattccc atagcaatgc cgatatcggc cttcttcaag gccggggcgt
      601 cattaacgcc atcaccggtg acggcgacga tctctcccat ctgtttcagc aaggaaacga
      661 tccgtagttt atgctccggc gcgacgcggg cgaagatggt ttcctgctgc gacagaattt
      721 cgcgcaacgc gttatcgtcc agctcggaaa cttcggcgcc tgtgaaacat ttcagggatc
      781 cgttctgtcc aagaccgatt tgccggccga tgctggcggc ggtgagcggg taatcgccag
      841 tgaccatgag gacgcggatg cccgcggtat ggcagtcgcg gattgccccc ggaacctcgg
      901 cccgcaccgg atccgatagt gctaccaagc caagaaaaac taagtgcgtc tcgatgtaag
      961 ctgtcgtgta agcagcgccg tttaattccg ctccttctcg gtatgcaaat gcgagcaccc
     1021 gcaggccgcg ctgtgccatt gcgtcatttt cctcgagaat acgccggcgc tctgcgtctg
     1081 ttagcgggcg gatctcgcca ttccaagaga atcggtcgca ctgctggagc gtttcgagtg
     1141 gagcgccttt gacatagatc acggtctcgc tctgctggtg ggccttgatg accacgctca
     1201 tccgtttgcg gatcgattcg aaaggattta aatgcaggcg ttgatgaacg ccccgaagcc
     1261 cggctttttc ggccaggcac actaaagctc cctccgtggg gtcgccgatc acagcgtaat
     1321 ccgcgccttg tttgctcagc cgtgcgttgt tgcatgtgaa agcgcaatcc agcaagcggg
     1381 tcaaggtcgg ccaatgggag attgcatcca aagaaagcct tttccccgag gtgttaaatt
     1441 ctccttgcgg tcgataacca gcgccggaga cttggacgat cttgccgtca acggcaattt
     1501 cggtggccat catgagattt tgcgtaagcg tgccggtctt atcgcagcag atcaccgtag
     1561 tgcaccctag agtctcgacc gaagacaagt ttttcattag cgcatggcgc ttggccatgc
     1621 gttgcacgcc catcgccaga gataatgtca cggtcggcag taagccttca ggtacattgg
     1681 cgacgaaaag cccgatgcag aaaacgaacg ccgcgagaaa agtcaggccg gcgactaacc
     1741 agcccagcat tagaaaggct aatcccaggc caccggcgag cgcagcgatg gagtaaactg
     1801 tggcgcggag ttgtttctgt aaggggcttg gctcggctcg aatgtcccga gtcagattag
     1861 cgattttacc gatttctgta ttcatcccgg tgccgaagat cacagcccga gcgcggcctc
     1921 gcagcaaaga tgtgccggcg aagatgacat tgggtagctc gagccagaga aacttgcccg
     1981 gaatgagaat cggctgatcc gacttgtagc gacgagccgg agtggcctca ccggtcagcg
     2041 aggagttgtc tagctcaact tcgaaggcct ccagcaatcg tgcatcggcc ggtacgagat
     2101 caccttcttc aaccacgatc acatcgccgg gaacaagctg gcttgcatca cactcctgga
     2161 ggttgccgtc gcgcatcacc tgacaacgct gggtgatgag ttgctgtaga atttccaagg
     2221 aacggtcgga tttatattcc tgcagaaagg caaaaaggcc gttcaccaga atcacagcca
     2281 aaatggcgat tccgagttgc ggcaaatcga ctccgggaat gaaacagagc agggccgcta
     2341 tccaaagcag tatggcgaag aagctaaaca ggttgcgcag taatttcagc tgccaaggat
     2401 caacccgtga gcgttgcaac agattaggcc cgtagtggtg taaacgctgt tgcgcctcag
     2461 ttgacgtcag accgtgtgcg agattggttt ggagctcgaa gccgatctgc tcctgagtga
     2521 gattcaaata ggtcggcaaa gccgattgct gctcggcgcg cgcaatgatt ctaagcaccc
     2581 ggaccgcatc gcgttgcgcc aaaagttcgt cgcggatcgc cggcagcaac gagcagatcc
     2641 gctgtaagat ctgcagatgt tgcggtggct gctcggcggg cgtcgcaaga agcaggacaa
     2701 tgtggattcg atgattggca aaagaaaggc cctctgggga gatcccgagg atcaattccg
     2761 gcgcggcaag atgatcgatg cgcacatggg gaacggcaat tccgtgaccg atacaactat
     2821 agcgctcatt ctcgtccgat ttttgtgctc ggaaatgacc ggcgaggtca gccacagacg
     2881 atcgagcaca aagctcgtca atggctttga cgagggttcc cttaagctcg aaaacgatct
     2941 tttttgagtc gaggcggtcg gcaatcatgg tcccaaactg gatagccgat cgctatgcga
     3001 tagacgacgt tggagaacac ggccgatcga atgtagcaac gcagcactca gtaccatcag
     3061 aaagattcca agctggctga cggccaatct gccgatgggt tggccgatga tcatcaagca
     3121 cgcgatcagg gccaggtgtt tggcgagctt tatgagaaag gcgccgatca cgcacccgga
     3181 cgcgagaaac gacttcgtgc tagcaggctc gctttgctgc cgatggagca tgtgggaatc
     3241 ctcgcgagag agcatgccac cggctgatta ggcgatcgat cccaaataat gcaggacgtt
     3301 ggtcgtggtg accaaaccca ccagggttcc atcactgagg acgggcacac aaccgatttt
     3361 cttgttcgcc atgagcatcg cggcatcttt gatcggtgtg tcgagcgtca ccgttatgac
     3421 ccgttttttc ataatgttct tgatcaactc gcctttgagc aaagccgatc tgcttttctg
     3481 cttaagacca aaaatacggt tcgccgcggc gccgataaga tctcgtccag agatcaaccc
     3541 caccaacttg ccattatcta gaattggaat gtggcgaatg cgaccaagag aaattacgtc
     3601 attagctaga tctagagtat cgttgggggc taaagttaca ggcgacccca tcataatctc
     3661 ctttaccgtc cctttctttt catttttcgt tttcatgtga gcgctcctcc gacaggtgtt
     3721 gaggcgatcc agacaatgtt gcgcgccttc ttttatggaa ggaatgtgca ctgtccgtgc
     3781 caatcgtagc cggtagggtg ttctcggaat tagcgccggc gacaagttta ttggcaacgt
     3841 aagaatattc acgctatctc cgcatgagta agaaggacgc ggtagaaagc tttgcaatca
     3901 ccctttccgg tgacagtcga ccaaagttat tggcagtttg atcgaacaat gcctgttgcg
     3961 cgtgatgcct ggtgagcgct aactcactgc gctgcaattg tcgagtttgc cggacttgta
     4021 aacgggtatg gactagcaga tgtgcttaat gatccgcgaa ggtttgtcgc cctcccactg
     4081 gtgctcacaa cgcagcgcag agcagaatcg cggtattgag cggttgattg gcacgtaatg
     4141 cctggacctt gaggcactcc acaggaacta gtctcagtaa cattgtcgcc tctccaatcg
     4201 aaaatctcgg caagaacaat acgatttacg gtatcagtat tggtcgcgaa acaagctgca
     4261 gtatagcccg cgggttcaag aattgccgca gttgaaagat gttcaaatct ccggacatga
     4321 ccagatgtaa tttgagagcc ggagccgtct tgccgagcag ttgagagact ttgggcttgg
     4381 acgcctgcga caagaacgag catgatcatg atgataatgg ttttggcgat catcgcgtag
     4441 aacatcataa aagcctcctt tgtgcacatc tgacagtcgt catccgtttt ctccctccac
     4501 tcacgccaca gtctattttc tttcgcccca aagagacgtc tgcgttgtag ttatcattct
     4561 ggatcgtagt caggtcgagt ctttgtaaac ttcagtcacg cgtccagacc gggtattgtt
     4621 ttacaagtat tctaacgaaa cattcgcaat agagtataga gctgctacgc aaaaggccca
     4681 attgagaacc cgctacagat catagatggg cgtacgaatc gccacgtgct cgcccatggc
     4741 aatagcgctg attatgtcgg cacggctgac gattccgata accttgccgc cgtccgtaac
     4801 cggtacccgc ttgatagcgt gggtgatcat gagttgggcg atctcttcaa cgggtgcatc
     4861 ctcccggaca gtgacaactt ttttactcat gatcgatttg gcatccttgc ctttttttgc
     4921 cacgatatcc gcttccgaga cgatgcccag gattttaccg tgttcatcta cgactggggc
     4981 accgctgatc tgatttttga tcaagatcat ggctaagttc ctgaccttag tggtcgggct
     5041 tatggtaatg atgtcgcggg tcataacatc tttggcgaac attgtttgct cctcctagaa
     5101 tccttttttc
//
LOCUS       DASUTG010000815         5788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_718831, whole genome shotgun sequence.
ACCESSION   DASUTG010000815 DASUTG010000000
VERSION     DASUTG010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718831"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..700)
                     /locus_tag="VF089_18935"
     CDS             complement(176..700)
                     /locus_tag="VF089_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176092.1"
                     /translation="MSKVARHPRHPAVVNMSLEDISQRYTVRFADIKPDWSVFSDSNF
                     DGYRRAQFRYIGQTLGKPEVRHIPAGATRLSVMFVPPGEGNAPHTHPVEEVFFILKGN
                     VTVFFEEEDGRRVEENLGPWDCVSCPAGVIHGFHNNGLEPAYLQVMVGQTKPEPMGYA
                     DVKLDASDAQLARK"
     gene            1294..3627
                     /locus_tag="VF089_18940"
     CDS             1294..3627
                     /locus_tag="VF089_18940"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6176093.1"
                     /translation="MNSRKKFSSVGTSALRLDGAAKVTGATRYTADYSLPGMIWGKCL
                     RSPLPHARIRRLDVEKARNLSGVFSVLTAADLPDRLTGIILKDMPVLARDRVRFIGER
                     IAVVGAASPDIAEEALGRIEVDYDELPAVYDPVQAMAAGAPLIHEHLRCYDGLRPPLP
                     DIRNLHNYVEWRSGDHRQGFAEADFVFEQSFTTQRAHHGYLEPHAVVVQVEPSEKILI
                     WSTAKQVYLTRTNLAEWLGIEQEKIVFQVGAVGGDFGGKGTLMDLPLCYFLAQVTGRP
                     AKMIMSYAEELSAANPRHASVITIKTGVKKDGRLWARELKVVFNGGAYAGIKESSNGN
                     LPGSRHGNGSYAIPHSYIQSLCVYTNCIPGGIMRGPGDLQVNFAVESHMDSVARRLGM
                     DPYRFRRLNVLKPGDLLVSGQRVNTDMGQRLLEKMRRRIQPRGRSKKIPYVGRGLALA
                     SRDVNFGAANIDVGVSENGAAYILTTVPDTGTGAHTIFRQIVAEALTVSADDVAVRMG
                     TTEMFPTDVRVIASLVTYLSGQAVQQAAFALREKLIQLAATLLGCSPHAVRLERGAAV
                     GPKRARVNLADLAAKAKSTGGSLSVRGSIDIKERLGAMCFFAEAAEVTVDPETGQVKV
                     LKVISAHDVGTIINPLTHRGQVEGGMIQGFGFAMSEDLATEDGKVVTANLGEYKLPNI
                     RDIPEHETVYVRDHTGPGPFQAKQIGEHGAIPTAAAIANAVYDAIGVQITDLPITAAK
                     VFDALHQRAAAHRAEPTSAKSSARSTTADPIKIVGRH"
     gene            3630..4169
                     /locus_tag="VF089_18945"
     CDS             3630..4169
                     /locus_tag="VF089_18945"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176094.1"
                     /translation="MKRPLTQVGRSATRHDGQAKVSGAMRYAADIQRPGMIWGKCLRS
                     PYAHARIRSIDTTAAKEIPGVFAVLTADDLPRRLVGRRLKDMPVLAQQTVRFIGERVA
                     VIAAQTPEIAEESLSRIVVDYEEMKSVHDPIESMAAKAPLLHPNLGSYEILRLPLPDI
                     PNVHSRVQSVFGDVAQGFQ"
     gene            4179..4793
                     /locus_tag="VF089_18950"
                     /pseudo
     CDS             4179..4793
                     /locus_tag="VF089_18950"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            4796..5488
                     /locus_tag="VF089_18955"
     CDS             4796..5488
                     /locus_tag="VF089_18955"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6176095.1"
                     /translation="MDPIEFRYRNLLKRGDRLPYGVRLEYDKARQLLKRVAKKIGWNG
                     ARKRQPYVGQGLAFCIREIGIGEANVEVGLKDDGRVYLVTTVPDTGTGAHTILRQMAA
                     ETLGVAADDVEIIVGTTDHFPTDVAVAGSRVTYLAGQASQKAAGGLRDLLLNEAAETL
                     RCRVERLRFDKRFVRNGAKQKISLAELAARSASTGRKLQVKANFKIQKRGGTACFSHR
                     RQRSRSIRTPAK"
     gene            complement(5580..>5788)
                     /locus_tag="VF089_18960"
     CDS             complement(5580..>5788)
                     /locus_tag="VF089_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176096.1"
                     /translation="TAWRCTGQLAPRCVRLSVCYETVRVRLDPGIAEFQVAAHGRRRG
                     FVFAQVGPDDFTFFVAQIFHQREP"
BASE COUNT         1245 a         1717 c         1658 g         1168 t
ORIGIN      
        1 gtcagcggtc ccgacgagca gcacgacggt tttcccgttg aagaactcct gggcggcggc
       61 cggaactgcg gcgctcagga gtaatactgc gacaatgatc tttgcgctgt gccacattgg
      121 taaactcata gagtgtcctt gagcgtccgg aacttctcgg atgatttccc aggggctact
      181 tgcgtgcgag ctgagcgtcg ctggcgtcaa gcttcacgtc ggcataaccc atcggctctg
      241 gtttggtttg ccccaccatc acctgcaagt aagccggctc gaggccgttg ttgtgaaagc
      301 catgaatgac tccggcgggg catgagacgc agtcccaggg gccgagattt tcctccacgc
      361 gccggccatc ttcttcctcg aagaacacgg tcacattccc tttcaagatg aaaaacacct
      421 cttcgaccgg atgcgtgtgc ggcgcattgc cttctccggg cggcacgaac atcacagaca
      481 accgtgtcgc gccggctgga atatgtctca cctccggctt gccgagagtt tggccgatgt
      541 agcggaactg cgctcgacga taaccgtcga agttggaatc actgaatacg ctccagtccg
      601 gtttgatgtc ggcgaagcgg accgtatagc gctgcgaaat gtcttcgagg ctcatattga
      661 cgaccgcagg atgacgcgga tgacgagcaa ccttactcat gtcttgatcc tctaaaggtg
      721 ggtgaattac actgtttgtt cttatttgat gccgcactgt aatatcaaaa tccgtttacc
      781 ccccatgccc acaccttgtc aatgcataat cgtcgcgcta gacagagtct cccgccaagc
      841 cgccacggcg caaagttcaa gaggacaaca ttcccacgaa tgcgtgatga ctttaaggtc
      901 gcccagaatc agaatggagt cgtccttcaa gccgctaatt cgcgggcgtc tgttatcccc
      961 gcagggtcaa gcggtaatct aggcggaatt cgaccttcgc ccccgattaa agcattcggg
     1021 ggtgacgatc ttggggcgtc gcatctcttc accttagccg caatttcgaa ggagcacctg
     1081 aatgacacga acttcggaaa cgagagtgat gttatttttt tctgaattgt tattttggtg
     1141 agtgattgtt ctcgcaccta acgccttgcg gctgtttcga ttgcggcgat gcgacggcgg
     1201 gtacctcatg tttagccgat tatcgattga cagcctgcgc tgcttcactt tatcttgcgg
     1261 cttcgacaaa aaatgtttct cggacacttg gcgatgaact cgcggaaaaa attttcctcg
     1321 gtgggcacct cggcgctccg cctggacggc gcagctaagg tcaccggggc gacccgctat
     1381 accgcggact atagtttgcc gggtatgatt tggggcaaat gcctgcgcag tccgctgccg
     1441 cacgcgcgga ttcgtcgcct cgatgttgaa aaagccagga atctgagtgg cgttttttcg
     1501 gtgctcactg cggccgatct tcccgatcgt ttgaccggaa tcattcttaa ggacatgccg
     1561 gtgcttgcca gagaccgcgt gcgctttatc ggcgaacgga tcgcggtggt cggcgcggct
     1621 tccccggata ttgccgagga ggcgctaggc cgcatcgaag tcgactacga cgagttgccc
     1681 gccgtttacg atccggtgca agccatggcg gccggcgcgc cgctcatcca cgagcatctc
     1741 agatgctacg atggactgcg cccgccgctg ccggatattc gtaacctgca taactatgtc
     1801 gaatggcgct cgggcgatca ccggcaagga tttgccgaag cggatttcgt cttcgagcag
     1861 agcttcacca cccagcgggc gcaccacggc tacctcgaac ctcatgcggt ggtcgtccaa
     1921 gtcgagccgt cggagaaaat cttgatctgg tccacggcca agcaagttta tctcacacga
     1981 acaaacctgg ccgagtggct cggcatcgag caagagaaga ttgtcttcca agttggcgcc
     2041 gtcggcggcg acttcggcgg caagggcaca ctcatggatt tgccgctttg ctactttctc
     2101 gcccaggtca ccggtagacc ggcgaagatg atcatgagtt atgccgaaga gttgagcgcc
     2161 gctaacccgc gtcacgcctc agtcatcacc atcaagaccg gcgtcaagaa agatggccgg
     2221 ctctgggcca gagaactgaa ggtggtattc aacggcggcg cctatgccgg catcaaagaa
     2281 agcagcaacg gcaacctgcc cggctcacgt catggcaacg gctcctacgc gattccccat
     2341 tcgtacattc agtcgctgtg cgtctacacg aactgcattc cgggcgggat catgcgcggt
     2401 cccggcgatc tgcaggtcaa cttcgccgtc gaatcgcaca tggattccgt cgcgcgccgc
     2461 ctcggcatgg atccgtatcg gtttcgtcgc ctcaatgtcc tcaaacccgg cgacctgctg
     2521 gtcagcgggc agcgtgtgaa taccgatatg ggacagcgcc tgttggaaaa gatgcgtcgc
     2581 agaatccagc cgcgcgggcg ctcgaagaaa atcccttatg tcggtcgagg tcttgctttg
     2641 gcttcgcgcg atgtcaattt cggcgcggcg aatatagacg tcggcgtcag tgaaaatggc
     2701 gcggcgtaca ttctgacaac ggttcccgat accggcaccg gcgcgcacac aatatttcgc
     2761 cagatcgtcg ccgaggcgct tacggtttca gccgacgatg ttgcagttag aatgggcacg
     2821 acggaaatgt ttccgaccga tgtcagagtg atcgccagtc tggtcacgta cttgagcgga
     2881 caagccgtcc aacaggcagc cttcgcattg cgcgaaaaac tgattcagtt ggcggcgacg
     2941 ctgcttggct gttcgccgca cgcggtccgc ttggagcgag gtgccgcagt cgggcccaag
     3001 cgcgcaagag tcaacttggc cgatctcgcc gcgaaggcga aatcgacggg tggatccttg
     3061 agcgtgcgcg gcagcataga tatcaaagag cggctcggcg ccatgtgctt ctttgccgaa
     3121 gcggcggaag tcacggtcga tccggaaacc ggacaggtga aagtgctcaa ggtgatctcc
     3181 gcccatgatg tcggaaccat catcaacccg ctcacccatc gcggacaggt cgaaggcggc
     3241 atgatccagg gttttggctt cgcgatgagc gaagatctcg ccaccgagga cggcaaggtt
     3301 gtcaccgcca acctgggcga gtacaagctg cccaacatcc gcgacatacc ggagcatgaa
     3361 acagtgtacg tcagggatca caccggcccc ggcccgtttc aagcaaaaca aatcggcgag
     3421 cacggcgcga tcccgacagc ggcggcgatc gccaacgccg tctacgacgc aatcggcgta
     3481 cagatcaccg atctgccgat caccgcggca aaagttttcg acgcgcttca ccaacgggcc
     3541 gctgcacacc gggctgaacc gacatcggca aaatcgtctg ctcgttcgac aaccgcggac
     3601 ccgatcaaga tcgtcgggcg gcattgaaca tgaaacggcc gctcacccaa gtcggcagat
     3661 cagcaacccg tcacgacggg caagcgaaag tatccggcgc catgcgctac gctgcggata
     3721 tccaacggcc cggcatgatc tggggtaaat gcctgcgcag cccctatgcc catgcgcgca
     3781 tccgcagcat cgatactacc gcggcgaaag aaatcccagg cgtctttgcg gtcctcaccg
     3841 ccgacgattt gccgcgccgc ttggtcggcc gaagattgaa ggacatgccg gtgctggcgc
     3901 agcaaaccgt gcgtttcatt ggcgagcggg tggcggtcat cgcggcgcaa acgcctgaga
     3961 tagccgaaga gtcgctcagc cggattgtcg tcgattacga agaaatgaaa tcggtccatg
     4021 acccgatcga atccatggct gccaaggcgc cgcttctgca tccgaacttg ggcagctacg
     4081 aaattctccg tttgccgctg cccgatattc ccaacgttca ttctcgcgtt cagtcggtct
     4141 tcggcgacgt cgcgcaaggc tttcagtagt ccgagctgat ttttgaacag accttcacca
     4201 cgccgcgcgt gcatcacggc tatctcgagc cccacgctgt ggtcgtcgat atcgaagcgt
     4261 cggggcggat catcgtctgg tgtcctgcca agggtcctta cgtcactcga tcgcatctcg
     4321 ccgaatggct cgatttgccg gaataagaaa tctgcttcca gatcagtccg gtgggcggcg
     4381 acttcggcgg caaagcttcg ctgatggaca ttccgctctg ctactcgctg gccaaagcca
     4441 ccggccgccc ggtcaagatg atcatgagct acagtgaaga actgaccgcc gccaacccgc
     4501 gccacccggc gctgatcacg atcaaaattg gcctcaagcg cgacgccaga ctttgggcca
     4561 gggaagtcag agtgatcttc agcagcggcg tttacgccgg atttaaaaac agcgagaacg
     4621 tgaatctacc cggcgcgcgt cacggcgccg gccttatttc attccccatc tcaagatcga
     4681 cgcgttctcg gtttacacca attgcgttcc gagcgggatc atgcgcgcgc ctggcgaagc
     4741 tcagatgctg ttcgcggttg aaagccacac cgattttctt gccgcgcaaa tgaaaatgga
     4801 cccgatcgaa ttccgctacc gcaacctgct caagcgcggc gaccgactgc cttatggtgt
     4861 gcggctcgaa tatgacaagg cgcgccagct gctcaaacgg gttgcgaaga aaatcggctg
     4921 gaacggcgcg agaaagaggc aaccttacgt cggtcaaggg ctggcgtttt gcatccgcga
     4981 gatcggcatc ggcgaagcca atgtcgaggt tggcctgaaa gacgatggcc gagtttatct
     5041 ggtcaccacc gttcccgaca ccggcaccgg cgcgcacacg attctccgcc aaatggccgc
     5101 tgagacgctt ggggtcgccg ctgacgatgt tgagattatc gtgggcacca ccgaccattt
     5161 cccaaccgat gtcgccgtcg ccggcagccg ggtcacctat ctcgcgggac aagcgagtca
     5221 aaaagccgcc ggcggtttgc gcgatctgct tcttaacgag gccgcagaaa cgttgcgctg
     5281 ccgggtcgag agactgcggt ttgataaaag gtttgttcgt aatggcgcca aacagaaaat
     5341 ctctctcgcg gagttagcgg cacgctccgc ctcgaccgga cgaaagctgc aggtcaaagc
     5401 aaattttaaa attcaaaagc gcggtggcac cgcctgtttc tcgcacaggc ggcaaagatc
     5461 aaggtcgatc cggacaccgg caaaataagt attctcaaaa tgctttccgc tcatgacacg
     5521 ggcaccgtga tcaatccgct cacgtatcaa gggcaggtcg aggcggcatg atgcagggct
     5581 tagggttcgc gctgatggaa aatctgcgcg acgaagaagg taaaatcatc aggaccaacc
     5641 tgggcgaata caaatcccct gcgccgccca tgtgccgcca cttgaaactc tgctattcca
     5701 ggatcaagac ggacccgcac cgtttcatag caaaccgata ggcgaacaca gcgcggtgcc
     5761 aactgccccg tgcatcgcca ggcagtcg
//
LOCUS       DASUTG010000816         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_421787, whole genome shotgun sequence.
ACCESSION   DASUTG010000816 DASUTG010000000
VERSION     DASUTG010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421787"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..>2721
                     /locus_tag="VF089_18965"
     CDS             212..>2721
                     /locus_tag="VF089_18965"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX6176097.1"
                     /translation="MLGPLFGGGIRRRLLLWGLSLFGIALLVMVGAGYSYMARQIRRD
                     AAALQSELASVTAEQIRTFVRRKIERFSDNANALSLYPLGSKEQQLLLSLLVKNDSSF
                     TDASIINAEGMEVVKASDRRVYFPADLSDQSKTPKFIKALTGDDFISRVYTSTQAQPY
                     VTLAIPLWGAAQSVAGVVSAEADLSFLWEAIGKIQFGTAGYAYLVDEHGNLIAHKDAA
                     LVLKRMNLQEVDGVRRFLNNPTRSDPTPAQEGTGLTGSPVLVTYAPVPELGWSVILEE
                     PIEAALANVEILKRSALVFLVVGLFFGAAIIAWVSQKTTRPIQQLRQHVATIGAGNLE
                     HRADIKTGDEIEELAAEFNKMTDALQNSYATLEQKVDQRTKEISALYSVTTAVNQSLA
                     LKEIADAVIAKITEIFHFETTRVFLFNHETDELELRSSFEVDTNQAAGVRTYKRGEGI
                     VGRVADSGEPMIFEDIRTNPRYAALSRSKATHTAKLSFFAVFPIKTQSRVFGTMLFNA
                     RSPRKLTTDETRLLTSMSEHLAVAFEKASLFRQSETRSQQLSVLNTIGTAVSQSLNLE
                     IVLREAIEKMVEALDFDACWIYIRDPIEEELRLEAYKGLDDDLARSMDRRKLTAGISG
                     NIYQTGERMVFEDIRSDATYQQLTSRNKVGTAGFSSGAGFPIKAKQKVIGVLHLASKA
                     KRRFLSDELKLIESIAQEIGVAAENARLFEQVNEKTAELGQMNDELQEANRAKSEFIA
                     AMSHELRTPLNVIMGNAELTGDGFFGEINAQQKKSMMQIRHHSQFLLKLVNDVLALSR
                     LDAKKMSIELAAVDIEDLVAHAQSQIEQLNRHNRLQVS"
BASE COUNT          708 a          669 c          727 g          617 t
ORIGIN      
        1 attggtcgat tctcaatagg aaagtctgtg gcgttaggtg gtcgtccatt agaggaatga
       61 gagcagggaa cggaattaat gtgagtgaga ctaagaaccg aggctgctcc ggctcaaagc
      121 cagcaatacg ttgggagctt ctcgttcgac taatctcgaa ggtggatgaa ctaatcccat
      181 cgaccatgcg tatgcgggat ttcggaaaaa aatgctaggt cctctttttg gcggcggtat
      241 ccggcgtcgc ctcctgctat ggggcctcag cctttttggc attgcactct tggttatggt
      301 cggcgccggc tattcttata tggcaagaca gatccggcga gacgccgccg cattgcaatc
      361 ggagctcgcc tcagtcacgg cggagcaaat tcgcaccttt gttcgacgca agatcgagcg
      421 gttttccgat aacgccaacg ctttgagtct ctatccgttg gggagcaaag aacaacagct
      481 actgttgagt ctcctggtca aaaacgacag cagcttcacc gatgcgtcga ttatcaatgc
      541 ggagggtatg gaagttgtca aggcctccga cagaagagtt tattttcctg ccgacctaag
      601 tgaccagagt aaaacgccta aatttatcaa agcgctcaca ggcgacgatt ttatcagccg
      661 ggtctatact tcgactcagg cccagcccta tgtcacattg gccatacccc tttggggtgc
      721 ggcgcagagc gtcgccggcg tggtgtcagc agaagcggac ttatcgtttc tctgggaagc
      781 gatcggaaag attcagttcg ggactgcggg ctacgcctac ttagtagacg agcatgggaa
      841 tctcatcgcc cacaaagatg cggccctggt actgaagcgg atgaacctcc aagaagttga
      901 tggggtgaga agatttctaa acaatccgac gcgttcagat cccactcccg ctcaggaagg
      961 gacgggccta accggcagcc ctgtgctagt cacgtatgcg cctgtacccg agttaggctg
     1021 gtctgttatc ttggaggagc ccattgaagc ggcattagcc aatgtggaaa tactaaagcg
     1081 ttcagcgctg gttttcctgg tggtgggtct attcttcgga gccgctatca tcgcatgggt
     1141 tagtcaaaag actaccagac cgatccaaca acttaggcag cacgtggcga cgattggagc
     1201 cggtaatctc gagcacaggg cggatattaa aaccggcgac gaaatcgagg agttagctgc
     1261 tgaatttaac aagatgacgg acgctctcca aaactcgtat gcgactttgg aacagaaggt
     1321 ggatcagagg accaaggaaa tctccgcact ctatagtgtt acaacagcgg taaaccagtc
     1381 cttggctctc aaggaaatcg cggacgcggt aatcgcaaag attacagaaa tatttcattt
     1441 tgagaccacg cgggtgtttc ttttcaacca cgaaacagat gaattagagt tgcggtcatc
     1501 ttttgaagta gacactaatc aagccgccgg tgtccgcaca tacaagcgcg gagaggggat
     1561 cgtcggacga gttgccgatt ccggcgagcc aatgatattc gaagacatcc gcacaaaccc
     1621 acgctatgct gcgctaagca ggagtaaagc aacccataca gcgaagctga gtttcttcgc
     1681 ggttttccca ataaagacgc agtcacgggt cttcggcacg atgcttttca acgcccggtc
     1741 gccgcggaaa ctgaccaccg atgaaacgcg cttgcttaca tcgatgtcag aacatcttgc
     1801 cgtcgcgttt gaaaaggcca gtttgttccg ccagtcagag accaggtctc agcagctttc
     1861 cgttcttaat acgattggaa cagcagttag ccagtcgctg aatctcgaaa tcgttctaag
     1921 agaggccatc gaaaagatgg ttgaggcact cgatttcgat gcatgttgga tttatattcg
     1981 ggacccaatc gaagaagaac tacggcttga ggcttacaaa ggtctcgacg acgacttggc
     2041 ccggtccatg gatcgccgaa aattgacagc cggaattagt ggcaatatct atcagaccgg
     2101 cgaacgaatg gtctttgagg acattcggag tgacgcaacg taccagcaac tgacttctcg
     2161 aaataaggtt ggcaccgcgg gtttttcaag cggagctggc ttcccgatca aggcgaagca
     2221 aaaagtcatc ggagttttac atctcgccag caaagcaaaa cgccgcttcc tctccgacga
     2281 acttaaactc attgagtcca tagctcagga gatcggcgtc gccgccgaga atgcgcgact
     2341 cttcgaacag gtgaatgaaa agaccgctga gctaggccag atgaacgatg agctacaaga
     2401 ggcgaaccgg gcaaaatctg aatttatcgc cgctatgtcg catgagcttc gaactccact
     2461 caacgtcatc atgggtaatg cagagctcac cggtgacgga ttttttggcg agatcaatgc
     2521 ccaacagaaa aaatctatga tgcagatccg gcatcattcc cagtttctgc ttaaattggt
     2581 gaacgatgtg ttagcgttgt cgcggctgga cgccaaaaaa atgtcgattg aactggcggc
     2641 ggtcgatatc gaggatttag tcgctcacgc acaaagccaa attgaacaac ttaatcggca
     2701 taatcgtttg caggtatcct g
//
LOCUS       DASUTG010000817         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_866209, whole genome shotgun sequence.
ACCESSION   DASUTG010000817 DASUTG010000000
VERSION     DASUTG010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866209"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF089_18970"
     CDS             complement(<1..513)
                     /locus_tag="VF089_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176098.1"
                     /translation="MSRAEPSTTDLITPEEGWLLQREKLLAWLRLAFAIIAVVVIQLN
                     PSRTARFPTLSVFSLWSFLAYSLFILELVRRQKIISNRIGIITTCFDSVWIFLIVFST
                     GGTRTPFFFYFSFPVLSASLRWGTKGSVVVALVLVALYGLIRFSMAAESFNTPMGIDT
                     LLVRSIYLIAL"
     gene            complement(534..1238)
                     /locus_tag="VF089_18975"
     CDS             complement(534..1238)
                     /locus_tag="VF089_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6176099.1"
                     /translation="MMPIKVVIADDHALFRDGLKRILSLEKDILVVGEAANGDDVIKV
                     VERTKPDILLLDQKMPKGDVVQNLLEIGAKYPSTRVIILTAFSEEESVLNAAKGGARG
                     FVPKGVPATTLLQAIKAVHQGSSWTDKEIPSWETFEEIAQGQSLAREAPSQFDESIRS
                     LTKREMEILRLVAEGLTNEEIGKKIFISEKTVKTHLTNIFDKLKVNNRFKAALMLMGQ
                     SGETNASNWNRSGRGR"
     gene            1478..1948
                     /locus_tag="VF089_18980"
     CDS             1478..1948
                     /locus_tag="VF089_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176100.1"
                     /translation="MKDLFSISSAAAAVAVVFAISSCSTQDEMASKPAAAPAAPPAKA
                     EAKAESKAKAETTTAKAESKGEAKGEAKAKVEKARGDVGLLDTEKNYLILVTKEGKLI
                     TLDYDAKTKATQINPTPSKMAEVGLGSSATVAYSKQGEKNIASSIEFTPAKGGD"
     gene            1997..2368
                     /locus_tag="VF089_18985"
     CDS             1997..2368
                     /locus_tag="VF089_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176101.1"
                     /translation="MRTQSNLVRGLGIVCALSLASPILAQEEKKAGGEKKAEKAKAEK
                     VSGDIGLLDTEKNYMIVVTKEGKLVTVDFDQKTKVTELKPSPAKMADIGLGSSASVQY
                     TKKGEKNYLSSIEFTPAKGGD"
     gene            complement(2474..2932)
                     /locus_tag="VF089_18990"
     CDS             complement(2474..2932)
                     /locus_tag="VF089_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6176102.1"
                     /translation="MSKSENPKDAKARKDFLKSAAETLLEMKKQLHREMQVRVKGETE
                     GVKDEGRDTYDLASDERDREINFILNDREREKLLAIDEALERIKDKTYGICESCEGEI
                     QLGRLKVLPFTRLCVRCQEENEKESKRQKTLEEERGYRKLVLTDFEEEGS"
     gene            complement(2925..4265)
                     /gene="rimO"
                     /locus_tag="VF089_18995"
     CDS             complement(2925..4265)
                     /gene="rimO"
                     /locus_tag="VF089_18995"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX6176103.1"
                     /translation="MTQPKQKFNIVSLGCARNLVDSEVMSGLLQRDYYEMVQDPADAD
                     VVVINTCGFIDAAKAESIDTIVDISRLKQEGTLRKLVVAGCLSQRYPHELATELPEVD
                     VFIGTGEIPQIVDILEEHERNQSRRHYVGIPEYLYDHVTPRLRSTPSYTAFLKVSEGC
                     DHKCSFCIIPKLRGPHRSRSIESVVKEATMLAQSGVKEINLIAQDLTAYGRDRKDGTT
                     LYGLLNELVHVPDLVWIRLLYAYPNFLDSPLLELINKSEKICKYIDIPFQHVSAGILK
                     KMRRGKSGSAVQAAVEQLREAIPGLTLRTSLIVGFPGETEEDFQALLDFVEKTRFERL
                     GVFKYSIEEGTAAASLADHVDDEEKERRWQEVMDLQASISRTKNEALIGSIQRVMIDG
                     TDETGQSIGRTQGHAPEVDGVVYLQNPVVKSGDFADVKITEALEYDLIGEIQHV"
     gene            complement(4262..4924)
                     /locus_tag="VF089_19000"
     CDS             complement(4262..4924)
                     /locus_tag="VF089_19000"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX6176104.1"
                     /translation="MKLKRLIGALVIALSLAAQDWSVAAESKSAEQIVNALQKNYDAT
                     VDFVADFKQETEVKSLNRSLKAWGKLSFKRPGKMLWRYEEPKGQIVLTDGSHLYFLQP
                     EQNQVIKSPLKNAFRSDIPLSFLLGLGSLKNNFNAELKAAESDEYVLRLEPKGEAGAF
                     SEILLGVSRSSADIVWVSIRDAANNLTSLRFSNMKKGVGLQDSLFRINIPEGVDIVEL
                     GQ"
     gene            complement(4914..7157)
                     /locus_tag="VF089_19005"
     CDS             complement(4914..7157)
                     /locus_tag="VF089_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX6176105.1"
                     /translation="MEKGRLNREVWGVVISLTALLIIISLLSYSPHDRSLNTPSGLIA
                     HNWGGFVGAFLADLLLQGLGLSAYLLPVFLGLAAYQLFLPGFNGLRVSKAVGYVVLLV
                     GIAVLLSLLIDSEDARDSGGIIGGFLTESILVPLFGRISATLIILTTALLAIMTVSQV
                     SLADLLDRSGKRLLQLRKSIIPQIALRLSELKEPKDKPKRENAKNDRRDYVPPPIVVK
                     PDPKEELIKKVAKKPIAPQEQFKLPVVGEGYRLPPLELLDPPDPNELIKVDTNSLHAN
                     SLILQKKLADFGVDGEVVAVRPGPVITMYEFKPADGVKVRRIVMLADDLAMALRAVSV
                     RILAPIPGESVVGIEIPNPRRETVYLREIIESDSYNSVDSKITLALGKDIGGVPFATD
                     LGRMPHLLVAGATGTGKSVSINAMILSILFKATPQDVRFIMVDPKMLELTVYEEIPHL
                     LVPVVTDPKKAAAALFWAMDEMDRRYRLMRDKGARNIDNYNRTLEKESAVKKPVIELT
                     EAQTEEDPNAPGGSLEKEEPLVHERLPRIVIIIDELADLMMTVGRDIEEYITRLAQKA
                     RAAGIHLILATQRPSVDVITGLIKANFPARISFQVTSRIDSRTILDSMGGEKLLGNGD
                     LLFLPPGTARLTRIHGAFVSDQEVRKVMKFIKQQGRPQYRPEVFEVKKDIESAGADEE
                     YDEMYDQAVAIVTDTQQASISMIQRRLRVGYNRAARMIEQMERDGVIGPADGAKPREV
                     YARKLEA"
     gene            complement(7174..>7444)
                     /locus_tag="VF089_19010"
     CDS             complement(7174..>7444)
                     /locus_tag="VF089_19010"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6176106.1"
                     /translation="VLAVLAIHQQSGEIVAGPDLISRGFVRDEEGEEILEQARKVVLE
                     TFGGMNRETRADRAELQEEIRIALRRYFRKKLERRPVVLPYIVET"
BASE COUNT         1775 a         1963 c         1868 g         1838 t
ORIGIN      
        1 tagggcaatg agatagatgc tgcgcacgag caatgtgtcg attcccatcg gcgtattaaa
       61 gctctccgca gccatgctaa agcggatcaa accatacagg gcaaccagga ctaaagccac
      121 gacgacgctt cctttggttc cccagcgtag gcttgcggag aggaccggga aagagaaata
      181 gaaaaaaaac ggtgtccgtg tgccaccggt cgaaaacaca atcaaaaaaa tccataccga
      241 atcaaagcaa gtcgtaataa tcccaattct attggagatt atcttttgtc ggcgaactaa
      301 ctctagaata aataggctgt aagctaagaa cgaccagagg gaaaaaactg aaagtgtcgg
      361 aaagcgtgcg gtgcgtgaag gattaagctg tatgacgact acggcgatga tggcaaaagc
      421 cagccgcagc caggccagaa gtttctctcg ttgtaagagc cagccttcct cgggggtaat
      481 cagatcagtc gtggacggct cggcgcgaga catgagcttt tgaaatgaga tcgttaacgg
      541 cctcgaccgc ttctgttcca attggatgcg ttagtctcac cggactggcc catcagcatc
      601 aaagcagcct tgaagcgatt attaaccttt aacttatcga agatattggt caggtgagtc
      661 tttaccgtct tctcactgat aaagatcttt ttgccgatct cctcattggt tagaccttcg
      721 gccactaacc ggagtatttc catctctcgt ttagtaagac ttctaatact ttcatcgaat
      781 tgactcggcg cttctctggc aagcgactgt ccctgagcaa tctcctcgaa agtttcccat
      841 gagggtatct ctttgtccgt ccaagaagac ccttggtgca ccgccttaat cgcctgaagc
      901 aaggtagtgg cgggcacgcc ctttggaacg aaccccctag cgccgccttt cgccgcattc
      961 agaacacttt cctcctccga gaaggcggtc aaaatgatga cccgggtgga cggatactta
     1021 gcgccgattt ccaacaagtt ctgaacgacg tcgccctttg gcatcttctg gtcgaggagc
     1081 aagatgtccg gcttggtgcg ctcgaccact ttgatcacat catcaccatt ggccgcttcg
     1141 ccaacgacaa ggatgtcctt ctccagcgaa aggattcgct taagtccatc tctaaacagc
     1201 gcatggtcgt cggcaatcac aactttgatc ggcatcattg cactcgctcc ttgagggaac
     1261 attgcctatg ccaagcgaaa atacaacgaa ataaaccaac atgccaacgc ggtactcacc
     1321 gtctgaggca gggtcctacg actatggttg ggatttaggc caagtcagca tgacccctcc
     1381 agataaaacc atacctaatg cgtattgaga tgaatcaacg ctcaagataa tgatcgacgc
     1441 taaatctgct cgttaaatat gttctaggga ggcaacaatg aaggatttgt tttcgatcag
     1501 tagtgctgcg gcggcagttg cggtggtttt tgctatctct tcttgttcaa cacaagacga
     1561 aatggcttcc aagcccgcgg cggcacctgc ggcgccgccg gccaaagctg aagcaaaagc
     1621 tgagtctaag gcaaaagctg aaactactac ggcgaaggcc gagtcaaagg gcgaagccaa
     1681 gggtgaagcg aaagccaaag tggagaaggc gcgaggcgat gtcggactgc ttgataccga
     1741 aaagaattat ctgatcctcg ttaccaaaga aggtaagctg attacgctgg attacgacgc
     1801 taaaaccaag gcgactcaga ttaatcccac cccgagcaaa atggcggagg tgggattagg
     1861 ttcttcggcg acggtcgcat attctaagca gggtgaaaaa aatatcgcca gtagcattga
     1921 attcaccccg gctaaaggcg gcgattgatc cgatgcaagt ctcacgtcta tttaactatt
     1981 atgaaaggat ccatttatga ggacacaatc taacctagtg cggggtctgg gtatcgtgtg
     2041 cgcgctctcg ttggcttcgc cgattttggc gcaggaagag aagaaagcag gaggcgagaa
     2101 aaaagccgaa aaagccaagg ccgaaaaagt gtccggtgat atcggcttat tggataccga
     2161 aaagaactac atgattgtgg tgacgaagga aggcaagctg gttacggttg atttcgatca
     2221 gaagaccaaa gtcacagaac tgaaacctag tcctgcgaaa atggctgata tcggtctagg
     2281 ttcgtcagcc tcggttcaat acaccaagaa aggcgagaag aactatctta gcagcattga
     2341 gtttacccct gccaagggcg gcgattaatc gtccaaactt aaatggtaaa aggggcatgg
     2401 tatccaaacc atgccccttt ttttgctctt tgatatggcc gcggaaagtt gacccggaac
     2461 gggttctgat gagttaagag ccttcttctt cgaagtcagt cagaacaagc ttgcggtagc
     2521 ctcgctcttc ctcgagcgtt ttctgacgtt tgctttcctt ctcgttctct tcctgacacc
     2581 tcacgcagag tcttgtgaag ggcagcactt tcaagcgccc gagctggatc tcgccttcgc
     2641 agctttcgca gataccgtag gtcttatctt tgatgcgttc gagggcttcg tcgatggcaa
     2701 gcagtttctc ccgctcacga tcattgagga taaagttaat ttcccgatcc cgctcgtcgc
     2761 ttgctagatc gtacgtatcg cgaccctcat ccttaacgcc ttcggtttcg cccttcaccc
     2821 gaacctgcat ttctctatga agctgctttt tcatctccag caaagtttcc gcagcgcttt
     2881 taaggaaatc ttttctggct ttagcgtctt tagggttttc cgatttagac atgttgtatt
     2941 tcaccaatca aatcatactc aagagcttcg gttatcttta cgtcggcgaa atcgcctgat
     3001 ttcacaacgg ggttctgcaa gtacacaacg ccgtcaactt ccggcgcatg accctgtgtg
     3061 cgtccgatcg actgccccgt ttcatccgtg ccgtcgatca taacccgctg aatagacccg
     3121 atcaaggcct cattctttgt tcgtgagata cttgcttgaa gatccattac ttcttgccag
     3181 cgtcgttctt tctcttcgtc atcgacgtga tcagcaaggc ttgcggcggc agtgccttct
     3241 tcgattgaat acttaaagac cccgagacgt tcaaatctgg ttttctcaac gaagtcgagg
     3301 agcgcttgaa aatcctcttc agtttctccg ggaaagccga caatcagcga ggttcgaagg
     3361 gtcaaaccag gaatggcctc ccgcaattgt tctacggcag cctgcacggc actgccgctt
     3421 ttgcctcggc gcattttctt caagataccg gcgctgacat gctggaaagg aatatcaatg
     3481 tacttgcaaa tcttttcact cttgttgatc agctccagca gcggcgaatc gagaaaattg
     3541 ggataagcat agaggagccg gatccatacg agatcgggca catgaaccag ctcgtttaac
     3601 agaccgtaga gagtcgtccc gtctttgcga tctctgccgt atgcggtgag atcctgggct
     3661 ataagattaa tctccttcac accgctttgg gccaacatag tcgcttcttt gaccacggat
     3721 tcgattgagc ggctacgatg tggtccgcgc agcttaggaa tgatacagaa ggagcactta
     3781 tgatcgcatc cttcagagac ctttaaaaag gcagtgtagg acggagtact gcggagccga
     3841 ggtgtcacat ggtcgtatag atactcggga atgccgacat aatgtctgcg gctttgattt
     3901 cgttcatgtt cttcaaggat atcgactatt tgtggaattt cccccgtgcc tatgaacaca
     3961 tcgacctcag gtagctcggt tgctagttcg tgcgggtatc gctgcgatag acagccggca
     4021 accaccagct tccgcagtgt tccttcttgt ttcagtcggg agatatcgac gattgtgtct
     4081 atcgactccg ctttagcagc atcgataaag ccgcatgtgt tgatcacgac gacatccgca
     4141 tccgccgggt cctgaaccat ttcataataa tcccgttgga gtagtccgga catcacttcc
     4201 gaatcgacca gattgcgggc gcatcctaag ctgacgatgt taaacttctg ctttggttga
     4261 gtcactggcc caactccact atgtcgactc cctcgggaat gtttattcga aacaatgaat
     4321 cctgaagccc tacacctttc ttcatattgg agaaacgtaa gcttgtcaga ttgttggcgg
     4381 cgtcgcggat gcttacccag acgatatcgg ctgagctccg gctgactccc aggagaattt
     4441 cgctaaaggc tccagcttcg cccttaggtt ccaggcggag aacgtattca tcgctttcag
     4501 cggcctttaa ctcggcattg aaattgtttt tcaagctgcc cagcccaagt agaaacgata
     4561 gcgggatatc cgagcgaaag gcgtttttca acggactttt aattacctgg ttctgctcag
     4621 gttgtaggaa atagaggtgg cttccatctg tcagtacgat ttgccccttt ggctcctcgt
     4681 accgccacag cattttgccc ggccgtttaa acgacaactt tccccatgcc tttaagctgc
     4741 ggttaagact tttaacttcg gtttcctgct tgaaatcggc gacaaaatcg acggtagcat
     4801 catagttctt ttgcagtgca ttgacgattt gctcggcact ttttgattcc gccgcgacac
     4861 tccaatcctg tgcggccaag gacaaggcaa tcaccagagc cccaatcagt cgtttaagct
     4921 tcaagttttc tcgcatacac ttctctcggc ttggcgccgt ctgccgggcc gataaccccg
     4981 tcgcgttcca tctgctcgat cattcgcgca gcccggttgt atcctacccg caatctccgt
     5041 tgaatcatag agatcgaagc ctgctgagta tcggtgacaa tcgccaccgc ctgatcatac
     5101 atttcatcgt actcttcgtc ggcgccggcg gactcgatat ctttcttgac ctcaaatact
     5161 tcgggccgat actgcggtcg tccctgctgt ttgatgaact tcattacctt gcgtacttct
     5221 tgatcggata caaaggctcc atggattcgc gtcagccgtg ccgtgcccgg cggtaagaag
     5281 agcaaatcgc cgttgcctaa aagcttctcg ccccccattg agtccagtat tgtgcgtgag
     5341 tcaatccgcg aggttacctg aaacgagata cgggccggaa aattcgcttt gatcaaaccg
     5401 gtgatgacat cgacggaggg gcgctgagtg gcaaggatca ggtgaatacc cgccgctcgc
     5461 gccttctgtg caagtcgggt gatgtattct tcaatatcgc gtccaaccgt catcatcaga
     5521 tcggccagct catcgatgat gataacgatc cgtggcaaac gctcgtgcac cagcggctct
     5581 tccttctcca ggctcccacc gggtgcattc ggatcttcct ccgtctgcgc ttcggtgagc
     5641 tcaataacgg gcttcttgac ggcgctttct ttttccagtg ttcgattgta attgtcgata
     5701 ttgcgcgccc ctttgtctct cattagacgg taacgtcgat ccatctcgtc catggcccag
     5761 aaaagcgccg ccgcggcttt tttggggtcg gtcacaacag gcacgaggag gtggggaatt
     5821 tcttcgtaca ccgtcagctc cagcatcttc ggatcaacca tgatgaatcg aacgtcttgc
     5881 ggggtcgctt taaacagaat gctcaggatc atggcgttga tcgagaccga tttgccggtt
     5941 cccgttgcgc cggcgaccag cagatgaggc atgcgcccga gatcggtggc aaacggtact
     6001 ccgccaatat cttttcctag agctagagtg atttttgaat cgacggaatt atagctgtcg
     6061 ctctcgatga tttctcttaa gtaaaccgtt tcgcgccggg gattaggaat ttctattcca
     6121 accaccgatt ctcccgggat cggcgcgaga atacgcacgc tgacagcgcg cagcgccatt
     6181 gcgagatcgt cagccagcat gacgatgcgc cggaccttta cgccgtcggc gggcttaaac
     6241 tcatacatgg tgatgaccgg gccgggacgg accgcgacga cttccccgtc gaccccgaag
     6301 tcagccagtt ttttttgcag aatgagagag ttggcgtgca ggctgttggt atcgaccttg
     6361 attagctcat tgggatcggg cggatctaac agctccagag gcggcaagcg gtacccctct
     6421 ccgactacgg gcaatttaaa ctgctcttgc ggagcaatcg gctttttggc gactttcttg
     6481 atcaactctt ctttagggtc aggctttact acgatcggcg gcggcacata gtcgcgccga
     6541 tcgtttttag cgttttctcg cttcggtttg tctttgggct ctttgagctc ggagaggcgc
     6601 aaggcgatct gcggaatgat agatttcctc agctggagca gccgtttgcc gctgcggtca
     6661 agcagatccg ccagcgatac ctgtgaaact gtcatgatcg ccagtagcgc ggttgtcagg
     6721 ataatcaaag ttgcactgat gcgtccgaac aacggcacca gaatgctttc cgtaagaaaa
     6781 cctccgatga tgccgccgga gtctcgtgca tcttccgaat cgatcaatag gctcaaaaga
     6841 accgctatgc cgacgagaag cactacgtaa ccgactgcct tgctaacgcg aagtccgttg
     6901 aaaccgggga gaaataactg ataggcagca aggccgagga aaaccggcag caagtaggcc
     6961 gagaggccga gcccctgcaa caagagatcg gctagaaaag cgccgacaaa gcctccccag
     7021 ttatgagcga tcaaaccaga tggtgtattg agcgaacggt catgaggact gtaggacagc
     7081 aagctaatga tgatcagaag agcggtcagg ctaataacca cgccccagac ttcccggttg
     7141 agccggcctt tttccatggc agtaatgcga gccttaagtt tcgactatgt acggcaaaac
     7201 gacaggccgt cgttctagtt tcttgcgaaa ataacgacgt agcgcaatgc gaatctcttc
     7261 ctgcagctcc gctcgatcgg cgcgcgtttc ccggttcata ccgccaaagg tttctaagac
     7321 gacctttcga gcctgctcaa gaatctcttc accttcttca tcgcgtacga agccgcgaga
     7381 gatcaaatcc ggaccggcaa cgatttcccc cgactgctgg tggattgcca ataccgcgag
     7441 cacc
//
LOCUS       DASUTG010000818         7382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_274712, whole genome shotgun sequence.
ACCESSION   DASUTG010000818 DASUTG010000000
VERSION     DASUTG010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274712"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..964)
                     /locus_tag="VF089_19015"
     CDS             complement(275..964)
                     /locus_tag="VF089_19015"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX6176107.1"
                     /translation="MAENRKGKDKRFYFSRGQFVLLGCGFSVASMVVFFLGMLVGQGM
                     EERKIVKPTEPVMKIPIRPTHGANSATAGAKEEITFYDTLTRPAPAQPRPEEETREAK
                     RVERNSKAESRESRSPVRQEVASVAKRAEDKPAPAAGKPRSTEADAKDSRRPWTVQVN
                     AFPDEKSAKTWVDRLRDKGYNAYLSESRIQGRVWYRVRVGRFESRDEAEKTQESLKRK
                     EHLTKAFATSR"
     gene            complement(998..2800)
                     /gene="argS"
                     /locus_tag="VF089_19020"
     CDS             complement(998..2800)
                     /gene="argS"
                     /locus_tag="VF089_19020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX6176108.1"
                     /translation="MIVITVFFDRFPRIIESARHPLRRLIARADENQSIRPPMRKYLR
                     DLLTRAIEKTSKAGDLSSPEIPDLVIELPKQREFGDLSTNIAMHWARAAKKPPRVIAD
                     AIVKNIEDREGILARIDIAGPGFLNFTFSHKFYHQRLRDLVAGKDAQLDLGHGKKIQI
                     EFASVNPTGPLHVGHGRVAVIGDVLARLHEATGFDVEREYYVNDAGNQMENLGLSVYA
                     RYRELFGETFEFPEGGYPGEYVKDIAAEIKNQHGDKFLSEGQESAVDFFRSYGGDSLL
                     QTIRRQLSAFDIRFDSFFSEKAMRERGEIAESIRLLRERGLVYSKDGAEWLQSTAFGD
                     DKDRTVIKSDGELTYFAADIAYHRNKLERSFGKLINVWGADHHGYVPRLKASLRGLGY
                     DDSILRVVLVQMVQLTRAGEPVRMGKRTGEFVALQEVLDEVGRDAARFFFLMRKADSH
                     LDFDLDLAKQQSSENPVFYVQYAHARVASIFEQAHRSEIVWNAADLGTIAVEPLQLPE
                     EIELIRRMAQFNDVLEESVRELEPHRMVFFLLDLAGEFHRYYNRTRILSEDRELTWAR
                     MLLAHMVQLTIRRGLTLLGIDAPMKMISRSDVER"
     gene            complement(2899..3384)
                     /locus_tag="VF089_19025"
     CDS             complement(2899..3384)
                     /locus_tag="VF089_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012807094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX6176109.1"
                     /translation="MYLRTLVILTVLGLIAIFSALNWSAFIAPTVLSLGFTSVEAPLG
                     LILLGIVALLTLLFLVYITYLQSTVLLESRRHARELQSQRDLAEQAETSRFNQLRSLT
                     ETELEKLAADIEQSKSLLLTRLEQIDRDFRAALEQTANSLAAYIGELEDKFDSSAGKT
                     T"
     gene            complement(3729..4622)
                     /locus_tag="VF089_19030"
     CDS             complement(3729..4622)
                     /locus_tag="VF089_19030"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6176110.1"
                     /translation="MKTLGFIGTGGMGSGMAGNLIKAGHRLVIHDLRREQGSSLENQG
                     AVFKESPRAVAESCDIVMSMLPYNEAVQAVGLGNGGLHQASGGAKLWIDFSSIDKKTI
                     VDVANQLAQKGWTVLDGSAGGVEEAAAAGTLSLWISGSKELFDQHQPVFNAMGNKVLY
                     VGDLGNAKLVKNAMAMFAAVQHLSLVEIAAWLQKGGLSEETFRTVLKNSQQNSVAIDR
                     ICEIVVSRKFKPRKSWMPKDVGFGLDMARQMEVPMPFVALAYQMFSIAQATGSDGYEA
                     TGIACNVYDLITGRNQSQSKA"
     gene            complement(4642..5025)
                     /locus_tag="VF089_19035"
     CDS             complement(4642..5025)
                     /locus_tag="VF089_19035"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176111.1"
                     /translation="MHTGFRTSIKDIVDIHLVEDGVNRLSWRDFGNGLSMARLAREGK
                     RELVLYRIAADAAPDAFLKHEHVGGEFYLVLKGRIEDETGRSEQGDVVYLDAHSVHTP
                     RATGDTLVLVLWPEGVRLVSEEKAE"
     gene            complement(5162..5737)
                     /locus_tag="VF089_19040"
     CDS             complement(5162..5737)
                     /locus_tag="VF089_19040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6176112.1"
                     /translation="MENQQVYLIRHGETEWSLTGQHTGITDLPLTENGRTLAKRLEPI
                     LAKQKFVMVLTSPLKRARDTCELSGLGAAAQVDNDLVEWNYGEYEGLTSREIREKAPG
                     WLLFEHGCPGGETPEQVGNRADRIIAYARARQGNVALFAHGHLLRVIAARWLGFAAGA
                     GRHFLLDTGTLNILSYYQGIPAIKQWNRAIE"
     gene            complement(5876..6304)
                     /locus_tag="VF089_19045"
     CDS             complement(5876..6304)
                     /locus_tag="VF089_19045"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6176113.1"
                     /translation="MPFKRILSAVDFSPNSLEAFRIAAEMACVHSASLHLLHVIEAWP
                     ATPGEAAVEFAQQANTAIEQLVASSKPGLEKVTLTTEVASGRAFDEIVNRARDWRADL
                     IVLGTKGTTSLEEIFLGGTAEHVIKESPCSVLIVQPEDRA"
     gene            6532..>7382
                     /locus_tag="VF089_19050"
     CDS             6532..>7382
                     /locus_tag="VF089_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6176114.1"
                     /translation="MISIWDASTRKNRRLRADFDWRKTYRPVTGDCWNRSAMSVAGES
                     RNNRLEPPANGRPDHTLGDSNAPITLVEYGSYNCPSCLAANEIIADLRDRFGDRLRYI
                     FRHRPISGDPVARRAAELAQYAYETTGEYWPAHAALMKRGPSLRPEDFDALATELGLP
                     PTDVFHQEAWRLAQNKVQEDIDSARRSGARVSPTFFINNRRYEGPWDGSALTEALLGS
                     LGHRVQTAALDFVRWAPSTGLLLLTMIIAALLLTNSPAGPAFEALWEMPVGWNAGGRS
                     FQLPLRD"
BASE COUNT         1644 a         2157 c         1899 g         1682 t
ORIGIN      
        1 tctatagctg cgatcgatac ggagataacc ctagatgcga agtgacgcct tcatgcgatg
       61 cctcagacgt cgaccgctga tcccagccgg ccatcttcag cgcctggtcc gtgagctcta
      121 gcaggttgct gaaaaagacc cctatgcttc gttgccgctc aatcgcctcg ctccaacgta
      181 ctgtaagtac gcctccgctc gtcgattttt cgtgcgcctc gcctctggat ctttttgagc
      241 agcctgcaat agcctttttt caacaccctg ctagtcatct gctcgtggca aacgctttgg
      301 tcaagtgttc ctttcttttc aaggattctt gtgttttctc ggcctcatca cgcgattcaa
      361 aacggcccac ccgtactcga taccagactc ttccctgaat acgtgactca gataaatagg
      421 cgttgtagcc tttatcccgc agcctatcaa cccaggtttt tgcggatttc tcatcaggaa
      481 atgcgttgac ttgaacagtc cagggcctgc gagaatcttt agcatcagct tcagtagacc
      541 gaggtttgcc ggcagccggc gcgggcttgt cctcagcccg tttggccact gacgccacct
      601 cctgcctgac cggcgatcta ctttctctgg attcggcttt ggaatttctc tcaactctct
      661 tggcctctcg ggtttcctct tcgggccgag gttgcgcagg agccggtctg gtcaaggtgt
      721 cataaaaggt tatttcttct ttcgcgccgg cggtcgctga gttggcgcca tgcgtcggcc
      781 tgatagggat tttcatcacc ggttcggtcg gcttgacgat tttccgttct tccattccct
      841 gtcccaccaa catgccgaga aagaaaacaa ccatggaagc aacgctgaag ccgcaaccca
      901 gcaggacgaa ctggccgcgg ctaaagtaga agcgtttgtc cttacctttg cgattctctg
      961 ccatagatgc tgttccttca gtggtgggtg gcgatcgtca gcgttcgacg tcagatcgcg
     1021 aaatcatctt catcggagca tcgatgccca gtagggtcag accgcgccga atggtcaatt
     1081 gaaccatgtg cgccaacaac atgcgcgccc aagtcaactc tcgatcttcg gagaggattc
     1141 gagttcggtt gtagtaccgg tgaaactcac ctgctaaatc caacagaaaa aaaaccatgc
     1201 gatgcggctc gagctctcgg acactctctt ccagaacatc gttgaattga gccatccggc
     1261 ggatgagctc gatctcctcg ggcaattgca gcggctccac tgcgatggtc ccgagatctg
     1321 ccgcattcca gacaatttcg cttctgtgcg cctgctcgaa aatactcgcg acacgcgcgt
     1381 gagcatactg cacataaaat actggattct cgctcgactg ctgcttggcg agatccaagt
     1441 caaagtcgag gtggctatcg gccttgcgca tgagaaaaaa gaagcgcgcc gcatctcgcc
     1501 ctacttcgtc cagaacttcc tgcaaagcga cgaattctcc ggtgcgttta cccattcgca
     1561 cgggctcgcc ggctctagtg agttgaacca tttgcaccag aacaacgcgc aagatgctgt
     1621 catcgtaacc gagcccgcgc aaagacgctt ttaatcgggg cacgtagccg tgatggtcgg
     1681 cgccccagac gttgatgagt ttgccaaagc tgcgctcgag cttattgcgg tgataggcga
     1741 tatccgcggc aaaataggtg agctcgccat cgctcttgat caccgtccgg tccttatcat
     1801 cgccaaaggc ggtggactgg agccattcag ctccatcctt tgagtaaacc aaaccccgct
     1861 cgcgtaacag cctgatcgac tcagcgatct cgccccgctc acgcatggct ttctcgctga
     1921 aaaaagaatc gaagcgaata tcaaaagcgc tcaactgtcg gcgaatcgtc tggagcagag
     1981 aatcgccgcc atagctgcgg aaaaaatcta ccgcagattc ctggccctcg ctgagaaact
     2041 tatcgccgtg ttgatttttt atttcggcgg caatgtcttt gacgtattct ccagggtagc
     2101 cgccttcagg aaactcgaat gtctcgccga atagttcgcg atagcgcgca tacacggaca
     2161 gcccgagatt ctccatttga ttgccggcgt cgttgacata gtactcccgt tcgacatcga
     2221 agccggtggc ttcatgcaac cgagccagca catccccgat caccgcgacc cttccatggc
     2281 caacatgtaa ggggccggtc ggattgacac tggcaaattc gatctggatc ttcttcccat
     2341 gtccaagatc aagctgcgcg tccttacccg ccactaggtc gcgcaagcgt tgatgataaa
     2401 acttatgcga aaacgtaaag tttaaaaaac ccggaccggc aatgtcgatg cgcgcaagta
     2461 tcccttcccg gtcttcgata ttcttgacga tggcatccgc gatgactctc ggcggttttt
     2521 tcgctgccct ggcccagtgc attgcaatgt tggtcgacaa atcgccaaac tcccgttgtt
     2581 ttggcagctc aataactaag tcggggatct ccggtgagct cagatctccc gccttcgatg
     2641 ttttctcgat ggcgcgggtt aacaagtccc tgaggtattt tcgcatgggc ggtcgaatgg
     2701 attgattttc atcagctcgg gcgatcagac gccgcagcgg atgcctagca ctttcgatta
     2761 tacggggaaa gcgatcgaaa aacaccgtta taacgatcat ttataacggc ttacccggtt
     2821 ttaagtcaac gagaacccta gcttggcgcg tgagcccact tccggtcgat gcgccggatt
     2881 atctgagagc ggcacgtgct atgtcgtttt gccggcagaa ctgtcgaact tgtcctcaag
     2941 ttctccgatg taggccgcca aactgtttgc ggtctgttct aaagccgcgc ggaagtcgcg
     3001 atcgatctgc tccaatctcg ttaacagcaa agattttgat tgctctatat cggccgccaa
     3061 cttctccagc tcagtctccg tcaagcttcg caattgattg aaacgggagg tttcggcttg
     3121 ctcggccaag tcacgctgcg actgcagctc gcgtgcatgg cgccggctct ccagcagaac
     3181 ggtcgattgc agataggtga tgtaaacgag aaagagaagc gttaagagcg ctacgatccc
     3241 aagtaaaatc aaacccagag gcgcttcaac cgatgtaaac cctagagaaa gcaccgttgg
     3301 cgctataaaa gcgctccaat tgagcgccga aaaaatcgct attaggccca agacggttaa
     3361 tatcactaac gtgcgcaaat acatcgcata ccctcctgac ggctgagccc gccgttagac
     3421 cccacagaag caagatatcc taagagccct tattcagcac ggcattggaa agttcgaaga
     3481 gcaatgctcc actccctgag gttgcgatga agacgagcgc agcttcagat ttcactagca
     3541 ggctgctgaa aaaactccat atgcttcgtt gccgctcaat cgcctcgttg caacgtactg
     3601 taagtacgct tccgctaatc gatttttcgc gcgcctcgca tctggatctt tttgagcagc
     3661 ctgcaagagg ttttttcagc accctgctag taagagagac gctaaattcc gcgactatgc
     3721 cgtgggtgct atgccttgga ctgagattga ttcctgccgg tgataagatc gtaaacgttg
     3781 caggcgatcc cagtggcttc gtagccatca cttccggtag cttgagcaat cgaaaacatc
     3841 tgataagcca aggcaacaaa cggcatgggg acttccattt gacgcgccat gtccaatccg
     3901 aatccgacgt ctttgggcat ccaggatttg cgcggcttga attttcggct cacaacgatc
     3961 tcgcaaatgc gatcgatagc gaccgagttc tgctgtgaat ttttgagcac cgtgcgaaaa
     4021 gtctcttcgg aaagcccgcc cttttgtagc caggcagcga tttccaccag gctcaaatgc
     4081 tgcactgcgg caaacatggc catggcattc tttaccagtt tggcattgcc gaggtcgccg
     4141 acgtagagga ccttattacc catggcatta aacaccggct gatgttggtc aaataactct
     4201 ttagaaccgg aaatccacaa cgaaagagtt cccgcagccg ccgcttcttc aacgccgccg
     4261 gccgagccat cgagaaccgt ccaacccttt tgcgccaatt gattagcgac atcaacaata
     4321 gtttttttgt cgatactgct gaagtcgatc cagagcttgg ccccgccgct tgcctgatgc
     4381 aggccgccat tacctaaccc gaccgcctgc accgcttcat tgtacggcag catagacata
     4441 acgatgtcac aggattcggc caccgctcta ggtgattcct tgaacacggc tccctggttc
     4501 tccaagctgc tgccttgctc ccggcgtaaa tcatggatca ccaagcggtg tcctgccttg
     4561 atcagattgc ccgccatacc actgcccatg ccgccggttc caataaatcc tagagttttc
     4621 atctgacctc ccgcagcaaa ttcactcggc cttctcttcg gagacgagtc gcacaccttc
     4681 gggccacagc actaaaacta gagtatcgcc ggtagcgcgc ggcgtgtgaa ccgaatgagc
     4741 atccaggtag acgacgtcgc cttgctcaga tcggccggtt tcatcctcga tcctgccctt
     4801 caacacgaga taaaactctc cgcccacatg ctcatgcttc aggaaagcat cgggagcggc
     4861 atcggcggcg atgcgataaa gcaccagctc gcgtttgcct tccctcgcca atcgcgccat
     4921 tgacaggcca ttaccgaagt cgcgccagga taaacgattg acgccatctt caactaaatg
     4981 gatgtcgaca atatctttga tcgatgttcg gaaaccggta tgcatattcc ttcttgcgct
     5041 aaataacacg cagcttgcgc gaacgaaagc gcattgccag tggtgatgcg cagcgagtga
     5101 tgaaatagct aagttgaatc cgctgcatga tcgcggatcg gcaagcaggc tcgcagctcc
     5161 gctattcgat cgcccgattc cactgcttga tcgccggaat tccttgatag tagctgagaa
     5221 tgttcagagt cccggtatcg agaagaaaat gccgcccggc acccgccgcg aatcccaacc
     5281 agcgcgcggc gatgacccgg agcaagtgcc cgtgcgcgaa aagcgcgacg tttccctgcc
     5341 gggcacgcgc gtaggcgatg atgcgatcag cacggttacc cacctgctca ggagtttcgc
     5401 cgcctgggca gccgtgttca aagagcaacc agcctggcgc cttttctcga atctcacgcg
     5461 aggtcaatcc ttcgtattca ccgtaattcc actccaccag atcgttgtcg acctgcgcgg
     5521 cagcgcccag cccgctgagc tcacaggtat ccctggcgcg cttaagcggg ctggtcagaa
     5581 ccatgacaaa cttctgcttg gctaaaatcg gttccagccg cttcgccagg gtccgaccat
     5641 tctccgtcag cggaagatcg gtgattccag tgtgctgtcc agtgaggctc cactccgttt
     5701 cgccatgccg gattagatag acctgctgat tttccatatt gatattttgg attgccaccg
     5761 gatgaccttt tgggtcgatt tcatgcccgc ttgaaggcac agagcgagga atcgattgga
     5821 cccgatatat ttttccaacg aacgttgatc cgacagctgc agtagttttt atattttacg
     5881 ctctatcctc cggttgcacg attaaaacgg agcaaggaga ttccttgatc acgtgttctg
     5941 cggtgccgcc taaaaagatt tcctcgagag aagtcgtccc tttggttccc aacacgatga
     6001 ggtccgcacg ccagtcgcgt gcgcgattga cgatctcatc gaaggcgcgc ccgcttgcga
     6061 cctcggtagt gagtgtcacc ttttcgagac ccggcttcga cgacgcgact agctgttcga
     6121 tagctgtgtt cgcttgctga gcgaattcca cggcggcttc ccccggcgtt gccggccagg
     6181 cttcaatcac atgaagcaga tgcaaggacg ccgaatggac acaagccatc tcagcggcga
     6241 tccgaaaagc ttccagcgag ttgggagaga aatcgacggc actcaatatt cgcttgaacg
     6301 gcatggtggt ctcctgtcct gcttcaaaac taagttagct tacacagaaa cgaccggcga
     6361 aagtaggcag gcttcagcag caatgtcatt aggcctttgt cggttcagga cggatagcgc
     6421 aaaacccaag cccaaagggc gctcaatgaa gagcttataa ttcagcgcat ccgatctcca
     6481 atgctctaat cagatgcgcg cctgaaaaat attagacttt taacgaccac tgtgatttcg
     6541 atatgggatg cttccacgag aaaaaaccga cggctccgag ccgactttga ttggagaaaa
     6601 acataccggc cggtcaccgg cgactgttgg aaccgttcag ccatgagcgt tgccggcgag
     6661 tcacgcaaca accgactcga gcccccggca aatggccgac ccgatcacac cctaggtgat
     6721 agcaacgccc ccatcaccct cgtcgagtac ggcagctaca actgcccatc ttgcctggcg
     6781 gctaacgaaa tcattgccga tctgcgcgat cgcttcggtg atcgcctgcg ctacatcttc
     6841 cgccatcgac caattagcgg cgacccggtg gcgcggcgcg ctgccgagct ggcgcaatat
     6901 gcctacgaga ccacgggcga atactggccg gcgcacgccg cgctgatgaa gcgcgggccg
     6961 agcctgcgac cggaagattt cgatgcgctg gcaaccgagc tcggcctgcc gccgactgat
     7021 gtcttccacc aggaggcgtg gcgcctcgcc cagaacaagg tgcaagagga catcgacagc
     7081 gcgcggcgca gcggcgcgcg agtgtcgccg acgtttttca tcaacaaccg ccgctatgag
     7141 gggccgtggg acgggagcgc gctcacggaa gccttgcttg gctccctcgg tcatcgggtg
     7201 cagacggcgg cgctggattt cgtgcgatgg gcgccgtcca ccgggttgct gctgctgacg
     7261 atgatcatcg cagcgctcct attgaccaat tcgcccgcgg gtcccgcgtt cgaagcgctg
     7321 tgggaaatgc cggtggggtg gaatgccggc ggcaggtcat ttcaactgcc gttaagagac
     7381 tg
//
LOCUS       DASUTG010000819         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2202841, whole genome shotgun sequence.
ACCESSION   DASUTG010000819 DASUTG010000000
VERSION     DASUTG010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202841"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VF089_19055"
     CDS             <1..723
                     /locus_tag="VF089_19055"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX6176115.1"
                     /translation="EIEREKNSEPSVYDMTLAAIRHLHDDNPHGFFAFIENENIDTAS
                     HLSDIAAVIHDYREFDRAVALAYEFYKKYPRETLILVTSDHETGGLGFTMALKDLSST
                     KADNQAAASKTDLRKLQTITISLQKASRILGPAPTADAVDKLMREYFSGFRLAPEFKQ
                     SIIARQPPSRTLYVDPTAHNLGLMVANNTQAYWQTSSHTNNPVAVAALGVGSEQFKGY
                     YDNADFGKKLLALLDSSKTSLR"
     gene            complement(788..1912)
                     /locus_tag="VF089_19060"
     CDS             complement(788..1912)
                     /locus_tag="VF089_19060"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6176116.1"
                     /translation="MPTKESSAADVARYRQNYLVEMDGIELYRALAAAEKDEKRAVIF
                     RKMAQTEERHARRWVRLIETAGSQVPEYRPTIRVRVLAWMAKHFGTQRVIPIISSMEA
                     SDEPGYLNQPEAAGLPAQERAHSRALRAMDDGISTQDAIIGSERWHVISRGGSLRAAV
                     FGINDGLVSNFSLVMGFAGADARPEYILFAGVAGLLAGSFSMAAGEYVSMRAQRELFE
                     QQIAMEQQELEMSPKEEEEELSLIYQAKGIPEREAAMLARRIISNPKTAIDTLAREEL
                     GLNPSELGSPWTAALSSFLAFIVGALIPVLPYLFSSGQVAWYASGLLSCLALLGVGAI
                     ISIFTAKGPWISGLRMLGIGLLASAITYGAGWLFGVSVGG"
     gene            2088..3296
                     /gene="ilvA"
                     /locus_tag="VF089_19065"
     CDS             2088..3296
                     /gene="ilvA"
                     /locus_tag="VF089_19065"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX6176117.1"
                     /translation="MLKFADIEEARKRIREQIYTSPLSYSETISRMSSSKVFFKLDNL
                     QMTGSFKERGALNRLLTLTQDEAERGVIAASAGNHGMAVAFHSRRLHVESTIVMPVHA
                     PLIKVGRVRQYGAQTVLHGEDYDGAFTEAQRLSQERRLTFLHAFNDPTIIAGQGTIGL
                     ELYEQNPQLDAVIVPVGGGGLIAGIALVLKGLNPRIRIIGVQAEVIPSMKAALGKGAP
                     ISLPPATTIADGIAVRKVGETALDLVKKHVDEIVTVSEGEIANAVLLLLEIEKTIAEG
                     AAATPLAALINKKVNLTDKNIALIVSGGNIDVNLISRIIEKGLIQDGRLSRFSVVISD
                     RPGNLARLSQRIADLGANILQIGQSRGFGRIAIDQTEVELVVETAGLDHVEQIRTELS
                     KDGFQVRWES"
     gene            3529..>3750
                     /locus_tag="VF089_19070"
     CDS             3529..>3750
                     /locus_tag="VF089_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176118.1"
                     /translation="MKTIKKTLLMMVIATVLVAPAGCKKNENGEGSAEQAGRKIDSAL
                     QKAGQETGKLIEQLGESMQKVGDNMQDKTS"
BASE COUNT          933 a         1072 c          964 g          781 t
ORIGIN      
        1 gaaatcgaac gcgaaaaaaa cagcgagccg tcggtctacg acatgactct tgccgcgatt
       61 cgtcatctgc acgatgataa cccacatgga ttctttgcat tcatagaaaa cgagaacatc
      121 gataccgcca gccatctctc cgatatcgca gccgtgattc atgattaccg cgaattcgac
      181 cgcgccgttg cgctggcgta tgaattttac aaaaagtatc ctcgtgagac cttgatcctg
      241 gtcacgtcgg atcacgagac cggcgggcta ggctttacca tggcccttaa agatctaagc
      301 agcacaaagg ccgacaacca ggcagcggcc agcaagaccg atctgagaaa gctacaaaca
      361 atcacaattt cgcttcagaa agcatcgcgg atcctcggac cggcgcctac ggcagacgcc
      421 gtcgacaaac tgatgcgcga gtacttcagc ggatttagac tggcgccgga atttaaacaa
      481 tccatcatcg cgcgccagcc gccgtcgcgc acgctctatg ttgatccaac cgcccataat
      541 ctgggattga tggtcgccaa caacacgcaa gcgtattggc agacatcctc tcataccaat
      601 aatccggttg cggtcgcggc acttggagtc ggctcagaac aattcaaagg ctactatgac
      661 aacgccgatt tcggcaaaaa gttgttggcg ctactggaca gcagcaaaac ttcactccga
      721 tgatagcgac gtcgacgacg aaaccgctca agtgctagat ccggctctca accgagttcg
      781 gccaaactta cccgcccacc gataccccaa aaagccagcc tgcaccgtaa gtgatggccg
      841 acgccagcaa tccgatgccc agcattcgca gcccgcttat ccacggcccc tttgcggtaa
      901 agatcgagat aatcgcgccc acgcctaaca gtgctagaca gctaagcaac ccactcgcgt
      961 accacgcaac ctgtccagaa ctaaacagat aagggagcac tggaatcaac gcgccgacta
     1021 tgaaagccaa aaaagagctc agcgccgcgg tccagggcga tccgagttcg gaaggattca
     1081 agcccaactc ttcacgggca agggtatcga ttgcggtctt cggattggag ataatgcgtc
     1141 gcgccagcat cgcagcctct ctctcgggga ttcccttcgc ttgatagatt aacgagagtt
     1201 cctcttcttc ctccttgggc gacatctcca actcttgttg ttccatagcg atctgctgct
     1261 caaagagctc gcgctgcgcg cgcattgaga cgtattcacc cgctgccatc gagaacgatc
     1321 ccgccaataa tccggcgacg ccggcgaaca aaatatactc gggcctcgca tccgcgcccg
     1381 caaatcccat caccaggctg aaattcgaaa ccagtccatc gttgatgccg aaaacagcgg
     1441 cgcgcaggct gcccccccgc gatatcacgt gccaccgttc gctgccgata attgcgtcct
     1501 gggtggaaat cccgtcgtcc attgcccgca acgcgcggct gtgtgctctc tcctgagcgg
     1561 gcaatccagc tgcctccggt tggttcaagt atcccggctc atcgctggct tccatgctac
     1621 tgatgatcgg gatcactcgt tgggtgccga agtgctttgc catccatgcc aaaacccgga
     1681 cccggatcgt gggacgatac tccggcactt gagagcccgc cgtctcgatg agccgcaccc
     1741 aacgtcgcgc atgtcgctct tcagtctggg ccatttttct gaaaatcacc gcgcgcttct
     1801 cgtccttctc ggctgcggcc aatgcgcgat agagctcgat gccgtccatt tcgaccagat
     1861 aattttgtcg ataacgcgca acgtccgctg cgctgctctc ctttgtcggc atcacgcgtc
     1921 ctttccgaaa ggataaattg ccttgatcct cagtgtctat gaggggcttt gtctattgca
     1981 gaaatagctg gcccccgcac gcctcatcct tgccacacga cggcttgcct agtcaaattt
     2041 attcactcgc cttgcacgcg ttgatagttt cattctaggt tagcatcatg ctgaaatttg
     2101 ccgacattga ggaagcgcga aaacgcatcc gtgagcagat ttatacttcg ccgctctcct
     2161 attccgaaac cattagccgg atgagcagca gtaaagtctt tttcaagttg gataacttgc
     2221 aaatgaccgg ctcattcaaa gaacggggcg cgctcaaccg tttgctgact ttgacacaag
     2281 atgaggccga gcgcggtgtg attgccgcca gcgccggcaa tcacgggatg gcagtggcat
     2341 ttcacagcag gcgcctgcat gtagaatcca ccatagtgat gccggttcac gcgccgctga
     2401 tcaaagtggg acgggtgcgg caatatggcg cgcagaccgt attgcacggc gaagattacg
     2461 atggggcttt caccgaagct cagcggctga gccaagaaag acgattgact ttccttcatg
     2521 cgttcaatga tcccacgatc atcgccggtc aaggcactat cggccttgaa ctttatgagc
     2581 aaaatccgca attagacgcg gtcatcgttc ccgtcggcgg cggagggtta attgccggta
     2641 ttgcgcttgt cttgaagggg ctaaatccgc gcatccggat tatcggagta caggcagagg
     2701 tcataccatc gatgaaagcg gccctgggaa aaggcgcacc gatttcgctc cccccggcaa
     2761 ccacgattgc cgacggcatc gcagtgcgca aagtcggcga aacagccttg gatcttgtca
     2821 aaaagcacgt cgatgagatc gtcaccgtta gcgaagggga aatcgctaat gccgtgctgc
     2881 tattgttgga aatagaaaag accatcgcgg aaggcgccgc tgcaacccct ctggcagcgc
     2941 tgatcaacaa gaaagtcaat ttaaccgaca agaacattgc cctgatcgtt tccggaggca
     3001 atatcgacgt caatctaata tcgcggatta tcgaaaaagg actcatccaa gatggacgac
     3061 taagccgttt ttccgtggtg atctcggatc ggccgggcaa tctggcgagg ctcagtcagc
     3121 gaatcgccga cctcggcgcc aacatcctgc aaataggcca gagccgcggc ttcggccgaa
     3181 ttgccattga tcaaacggaa gtcgaactag tggtcgagac cgccggtttg gatcatgtcg
     3241 aacagattag aaccgaattg agcaaggacg gctttcaagt ccgatgggaa agttaatcgc
     3301 acgcctctta atcggttatg tacggtggcg ccgtaccgcc gtgatcaacc gagcgggaaa
     3361 ttcgagaatc aatgtcgctg atgactgaat ccacttgacc actctaataa agaataccgg
     3421 ggcagtcata tccttttgtg cgtcgattcc accttgccta gggattaacc tgctggttgg
     3481 caaatccgca actgagtatt ccattggcaa tggaggcgtg aattctctat gaagacgatc
     3541 aagaaaacac ttctgatgat ggtgatcgcg accgtactag tcgcgccggc aggctgtaaa
     3601 aagaacgaga atggcgaagg ctccgcagag caagccggca gaaagatcga cagtgcccta
     3661 cagaaggcgg gtcaagagac cggtaaacta atcgagcaac tgggcgaatc catgcagaag
     3721 gtaggagaca acatgcaaga taaaacgagt
//
LOCUS       DASUTG010000820         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_866993, whole genome shotgun sequence.
ACCESSION   DASUTG010000820 DASUTG010000000
VERSION     DASUTG010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866993"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..820
                     /locus_tag="VF089_19075"
     CDS             167..820
                     /locus_tag="VF089_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176119.1"
                     /translation="MKTYNTHYLFLLLILGACSSKSVSPGMTLLTGLNGYHDEMERLE
                     GQEARWPERQKVGNTIRTTFLMTMGGSKEFNRLVELDVKRREYLIALRANALRPDRAA
                     EVKQDMVGIDAEIEALATIVKGQVAARSTSQLPAEPQQVIEGVATVGLLTMAMDNFSP
                     NGARDVAVPSINVGGYTVIDQKNLTFVKTPEGQTFQCTTVLVQEEGAGINCAALAGK"
     gene            complement(1060..1335)
                     /locus_tag="VF089_19080"
     CDS             complement(1060..1335)
                     /locus_tag="VF089_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176120.1"
                     /translation="MALKQTRNKSPKGKKNRACPECGSVEIIPIVHGVPTPALQKLID
                     EGKAIIADREEWEGMSEWYCKACGCDWSGEWRRFKIPGGVNATRVKK"
     gene            complement(1588..>2239)
                     /locus_tag="VF089_19085"
     CDS             complement(1588..>2239)
                     /locus_tag="VF089_19085"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176121.1"
                     /translation="FAKRQTGKPIKMAVPGPMTVIDSTLDETYNDEPALAMDIAAAIN
                     LELLELQASGCDVLQIDEPAMTRYHEKVFAYGARALDRCIEGIRIPTIVHLCYGYPGG
                     ADRQHHYEYPELLEELMKTKIGGFAVEFARSGYDPAVLGICKGRKIMFGCVDPSDSPV
                     PPLETIVQRVRHALKYIDPANLLLAPDCGLMTISRELANAKAQLLVDVAKEVRKTI"
BASE COUNT          489 a          627 c          608 g          515 t
ORIGIN      
        1 cctagcgcat cgatcaattt gtgttctttc cccgagccaa atgtaactct ccaaagctgc
       61 agcatccgtg ctttctatcg agacctacca aaaagctcac acccgttttc tccggtgtgt
      121 taaaatctcg ctgatcttac tcgctcgaag acacggaagg atctctatga aaacttacaa
      181 cactcactac ttatttctgt tgttgatcct gggcgcttgt tcgtcgaaaa gcgtctcccc
      241 cggcatgacc cttctcactg gcttgaacgg ttatcacgac gagatggaac ggctcgaggg
      301 acaggaggcg cgctggccgg agcgacagaa ggtcggcaat accataagga caacctttct
      361 gatgaccatg ggcggatcca aagaattcaa taggctggtg gaactcgatg tcaaaagaag
      421 agagtatctg atcgctttgc gggcaaacgc gctgcgacct gatcgcgcgg cggaggtcaa
      481 gcaggacatg gttgggatcg atgcggaaat cgaggcgctc gcgacgattg tcaaaggaca
      541 ggtcgccgcg cgcagcacct cacagctccc ggccgagccg cagcaggtga tcgaaggtgt
      601 cgctaccgta ggtttgctga cgatggcgat ggacaatttc tcgccgaacg gcgcgcggga
      661 cgttgccgtt ccaagcatca acgtcggcgg ctataccgtc atcgatcaaa aaaacttaac
      721 ttttgtaaaa acgccggaag gccaaacttt tcaatgcaca acggttttgg tgcaagaaga
      781 gggagcgggg ataaactgcg ccgcgcttgc cggtaagtaa gcgactgccg caatgccgtc
      841 caaacccggc gagtcgcatc ctgctactgg cccgtccaac agttcagtga tcaaccgaga
      901 ctggatcggc atgatccgac cagccggctc ggctaatcaa tccgcttccg cttcgtgctt
      961 gaacttcgaa tatctaaatt gtctacgaaa gccgccgtac aggttcgaaa ccgcggaaaa
     1021 ctgtctcatc ttccgtgcag cgtgaggaaa tgcggagtgc tatttcttta ccctagtcgc
     1081 atttacaccg ccgggtattt tgaacctgcg ccattcgccg ctccagtcgc agccgcaagc
     1141 tttgcagtac cactcgctca tgccttccca ttcttcgcga tcggcgatga tcgccttgcc
     1201 ttcgtcgatg agtttttgca atgccggggt aggaacgccg tgaacgatgg gtatgatctc
     1261 aaccgagccg cactcgggac atgcgcggtt ttttttgccc ttggggcttt tattgcgagt
     1321 ttgttttagc gccatggtaa tctaagtttg gagcagcttc tgctcgaagg taagatgatg
     1381 agtcctttta cttcgcgccg cgcgctgcat catcgacgcg cggtccgggc gtgcacggat
     1441 caaagtccgg gctagcacga tccgcaacga ccgcctttgt cccccttagc gcagggcgaa
     1501 acacagaggg ggatctggat tcccgttttc acgggaatga cgacgaaagc ctctgaggaa
     1561 tcttcaacaa acttctgata ggtcaaccta aatcgttttg cgcacctctt ttgctacatc
     1621 gaccagcagc tgtgctttgg cattggcgag ttcccggctg atggtcatca agccgcagtc
     1681 cggcgctagg agcaggtttg ccggatcgat atatttgaga gcgtgacgca cgcgctggac
     1741 aatcgtttct agcggcggca ccggggaatc actgggatcg acgcagccga acattatctt
     1801 tcgccccttg cagatgccca gcaccgctgg atcgtaaccg cttcgggcaa actcaacggc
     1861 gaaaccaccg attttggttt tcatcaactc ttcgagcaat tccggatact cgtaatgatg
     1921 ttgccgatca gctccgccgg ggtaaccgta gcagaggtgc acaatggtcg gaatccggat
     1981 accttcgatg caacggtcga gcgcccgagc cccatacgcg aacacctttt catgatagcg
     2041 ggtcattgcc ggctcgtcga tttgtaatac gtcgcagccg gacgcttgca gctccaacag
     2101 ctctaagttg atcgcggcgg cgatgtccat cgcgagagcg ggttcgtcgt tgtaggtctc
     2161 atccaacgtg ctgtcgatca ccgtcatcgg tcccggtacg gccatcttga tcggcttgcc
     2221 ggtttgccgc ttggcgaaa
//
LOCUS       DASUTG010000821         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_570673, whole genome shotgun sequence.
ACCESSION   DASUTG010000821 DASUTG010000000
VERSION     DASUTG010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570673"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF089_19090"
     CDS             <1..772
                     /locus_tag="VF089_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6176122.1"
                     /translation="ATAVAFLLLILLVVLPILVLLLVAILPYYHVPTWQTWQNLTFDH
                     FRYIWATPRVHRAFVNSLFLALVGATICMLLASLASYISVRTKIAGRGLLEGLVFIPW
                     AFPGTAMALGLLWAYVDFPIPVYGTIWIILIAYVTRFLPYGLRAVSSTIIQVHKELEE
                     ASVVCGAGFFATFRRVLIPMMRPGVMAGWIILVTIFLREFSATLFLYSPGAEPLGPLL
                     YFLYLDGMRGRVAAIGLVISVISVILIAIAQRYSRWEN"
     gene            815..1807
                     /locus_tag="VF089_19095"
     CDS             815..1807
                     /locus_tag="VF089_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176123.1"
                     /translation="MEKLFLTVACGNYDRTFALQTGAIQAEGIRINYVPLEAEEIFWR
                     MGHHQEFDVSEMSLSNHVTMISRGDSPFVAIPVFPSRFFRHSCVFINTDSGIKSPADF
                     KGKKIGAPEYSITAAVWIRGFLNDDYGLKTSDMQWFTGGQEETGRKERVKLTLPPQIK
                     LEPIADDKTLNGMLESGEIDALISARSPSSFVKGSPKVKRLFPNYKDVEIDYYKRTRI
                     FPIMHVLVIRREVYEKNPWVARSLYKAFCEAKDVAIKNMHISNTMACTLPWLPWEREQ
                     LREIFGPDWWPYGVEANRHLLEHLIRYMDEQGLLARKLTVDEIFAPNVAGEFKI"
     gene            2089..2523
                     /locus_tag="VF089_19100"
     CDS             2089..2523
                     /locus_tag="VF089_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6176124.1"
                     /translation="MPTVSGLSPSALRFIRSARLAHLATAGLNSQPHVVPICFVFDGK
                     HFYSPIDEKPKRTGPAKLKRVINLQANPQAALVIDRYSEDWKNLAYALIYGEAKILYR
                     GAAHAQAVRRLRRKYPQYRQMAIHTRPMIQIRPIRWVLWGNP"
     gene            2771..3013
                     /locus_tag="VF089_19105"
     CDS             2771..3013
                     /locus_tag="VF089_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX6176125.1"
                     /translation="MQVRVQKWGNSLAVRIPKPLAEDADVKEGTVLNLTVSEGKVVAT
                     PVERKKLSLRQLLAKVTRKNLHAEVDFGPSVGREIG"
     gene            3013..3360
                     /gene="mazF"
                     /locus_tag="VF089_19110"
     CDS             3013..3360
                     /gene="mazF"
                     /locus_tag="VF089_19110"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HEX6176126.1"
                     /translation="MARRYVPARGDAVWITFNPQAGHEQAGRRPALVLSPSSYNGKVG
                     LAILCPITSQIKGYPFEVTIPSGSKLGGVILADQVKSFDWRIRKAEFICKLPRETTSE
                     ALDKLATLLLEDS"
     gene            3439..3636
                     /locus_tag="VF089_19115"
     CDS             3439..3636
                     /locus_tag="VF089_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176127.1"
                     /translation="MIDNDTQLHQAQADIQKLWRFLGSARQTHSSLDYERMAAPYLLQ
                     IQERQQEVLIYLSANPTAADA"
     gene            4227..>4720
                     /locus_tag="VF089_19120"
     CDS             4227..>4720
                     /locus_tag="VF089_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176128.1"
                     /translation="MTAASSRAWFSASIADFLETQPDAIVGRLATNNAFTLLLTQKDA
                     WLAQIGFLQAHLHGLTGSLFLEFNIPRMGRRIDTVLLIGPVLFVLEFKVGESAFERAS
                     VDQVWDYALDLKNFHEASHSVSIVPILIATGATKSTPSSLHADEDNVYRPILVHPTGF
                     REAI"
BASE COUNT         1074 a         1277 c         1220 g         1149 t
ORIGIN      
        1 ggccacggca gtggcatttc tactgctgat attgttggtc gtcttgccga ttctggtgct
       61 cctgctcgtc gccattttac cttattatca tgtgcccacg tggcagacct ggcaaaatct
      121 cacgttcgat cacttccgtt atatctgggc aacgccgaga gttcaccgcg ccttcgttaa
      181 cagtttgttt ctggcccttg ttggcgccac catctgtatg ctccttgcct ccctagcgtc
      241 gtatatttcg gttcggacga aaatcgccgg acgcggattg ctcgaaggtt tggttttcat
      301 accatgggcg ttccccggaa ctgccatggc tctggggctg ctctgggcgt atgtcgattt
      361 tcccattcct gtatatggca ccatctggat tatcttgatc gcctatgtga cgcggttcct
      421 tccctatgga ctgcgcgccg tcagcagcac aatcatccaa gttcacaagg agctcgaaga
      481 ggcttcggtc gtctgcggcg ccggattttt tgcgactttc cgccgggtgc tgattccaat
      541 gatgcggccc ggtgtcatgg caggctggat tattctcgta acgatcttct tgcgtgaatt
      601 cagtgccaca ctgtttctct acagtcctgg cgccgaacct ctaggtccac tgctttactt
      661 cctgtatctc gatggcatgc gcggccgggt ggccgccatc ggcctagtga tctcggtcat
      721 ctcagtgatt ttgattgcga ttgcccagcg ctattcgcgt tgggaaaact aactaactgg
      781 aatacctgtc tgttaatctc caggaggctc gttaatggaa aaactatttc tcaccgtcgc
      841 ctgcggcaat tacgaccgga cgttcgcgct ccaaaccggc gccatacaag ccgaaggcat
      901 acggatcaac tatgttcctc tcgaggcgga agaaattttc tggcgcatgg ggcatcacca
      961 ggaattcgac gtttccgaga tgtcactgtc caatcacgtg accatgatca gccgcggcga
     1021 ttcgccattc gtcgccattc cggtgtttcc gtcgagattc ttccgccact cttgcgtgtt
     1081 catcaataca gattccggca tcaagagccc ggcggatttc aagggcaaaa aaattggcgc
     1141 gccggaatac tcgattaccg ccgcggtgtg gattcgcggg tttttgaacg acgactacgg
     1201 gctcaaaacc tcggatatgc aatggttcac cggcgggcaa gaggaaaccg gcagaaaaga
     1261 acgtgtcaaa ctaacactgc cgccgcagat caaactcgag cccattgccg acgataaaac
     1321 cttgaacggc atgctcgaga gcggcgagat cgatgcgttg atttcggctc gcagtccttc
     1381 aagtttcgtc aagggctcgc cgaaggtcaa aagattattt cccaactata aagatgtcga
     1441 aatcgactac tacaaacgca cccggatctt tccaatcatg catgtgctgg tgatccgcag
     1501 agaagtttac gaaaagaacc cctgggtcgc gcgcagcctt tacaaggcgt tttgcgaagc
     1561 gaaagacgtg gccattaaga acatgcacat ttcgaatacc atggcttgca ctcttccctg
     1621 gttaccatgg gaacgcgaac agttacggga aatattcggc ccggattggt ggccttatgg
     1681 agtggaagcc aaccgtcatc tgctcgagca tctgatccgc tacatggatg agcaaggatt
     1741 acttgcgcga aagctcaccg tcgatgagat cttcgcgccg aatgtcgccg gagagttcaa
     1801 aatctagcag gctgctgacg aagactcata tgcttcgttg cgcttgttcg cttcgcttcg
     1861 acgtactccg acgtacgcct cagctcatcc aacttcgcgc gcccggcctc tgagatcttt
     1921 ttgagcagcc tgcatttcaa agtttttcag ccgagtgctt gtaggcagga ggcacctagg
     1981 cgacagcaag tcggccggca aaccgtcctc ctgcactgat acgatactcg aacctcatgc
     2041 tgcctccttc tattgtctag tgcctatttg cctaatgcct atcgtgtgat gcctactgtc
     2101 tcaggtctct cgccttcggc attgcggttt attcgtagcg cgcggcttgc gcatctcgct
     2161 accgctggcc ttaacagtca gcctcacgtc gtacctatct gctttgtttt tgatggaaaa
     2221 catttctact cgccgattga cgagaagcct aagcggaccg gcccggcaaa gctcaagcga
     2281 gtaatcaacc ttcaagctaa tccgcaggcc gcgctggtga tcgaccgata cagcgaagac
     2341 tggaagaacc tcgcctatgc tttgatttac ggagaggcaa agattctcta tcgcggcgcc
     2401 gctcatgctc aagccgtgcg ccgcctgcgc aggaaatatc ctcaataccg acagatggcg
     2461 atccacacca gaccgatgat tcagattagg ccgatccgct gggttttgtg gggtaatccg
     2521 tagagggaac actcagcaag caaccggact ttcctccaaa ggacttgcat ctgttagctc
     2581 cagtttatgg gcagcgaacg aggcaggatc gtgacgcgct cagtggcgcc gctggtgaag
     2641 gacttggaag cacgtttaag aagcaagagt tttgaccgcc tatcttcgtt ctgtcattgt
     2701 tatgcaatgt gcttgacgcg aatcttgcag ctctgtatct tcattgtaga aacaggctgg
     2761 aggtgcattg atgcaggttc gagttcaaaa gtggggaaac agcttggctg ttaggattcc
     2821 caagccgctg gctgaagacg ccgatgtgaa ggaaggaaca gtgttgaact tgacggtttc
     2881 cgaaggcaaa gtcgtggcga cacccgttga aagaaagaaa ctgtctctca ggcagttgct
     2941 cgcaaaagtc actcggaaaa acctgcacgc ggaagttgat ttcggcccgt cggttggacg
     3001 tgagattggg taatggctcg ccgctacgtt ccagcccggg gcgacgcggt ctggatcact
     3061 ttcaatcctc aagccggcca cgagcaagct ggtcgtcgcc cggccttggt tctttcaccg
     3121 tcttcttaca atggtaaggt tggactcgct atcctttgcc cgattacgag tcaaataaaa
     3181 gggtatccgt ttgaggtaac gatcccgtct ggatcgaagc tcggcggcgt gattcttgcc
     3241 gatcaagtta agagtttcga ttggcgtatt cggaaagcgg aattcatctg caaactgccg
     3301 cgcgaaacca ccagtgaagc cttagacaag ttagcaacgc ttttgctcga agacagctag
     3361 ccggcttgaa caccgaagag atgtttgcgt tcatcagaca tcgtttgaga attgatcgag
     3421 taagtgagag gtgcagccat gatcgataac gacacacaac ttcatcaggc gcaggctgat
     3481 attcagaagc tatggagatt cttgggaagc gcgcgccaaa ctcattcaag tctggactat
     3541 gaacggatgg cggcgcccta tctcctccag attcaagaac gtcagcaaga agtactcatt
     3601 tatctatccg ctaacccaac ggcagcagac gcataatcgg ttgggcaact tagcaattcg
     3661 ggcatcgatc cccgtctgaa gttgaacgac gtttcagcga gatctggaga gaatcggcgt
     3721 ccgcctgacc tctataatca aaaaaacccg gccaatcctg tgcctccacg gtgatcccag
     3781 cgactctaaa agtgatgtcc tgctcctcac ccccacgcat cagggcattt tcaaagttct
     3841 ttggtgtagg acttagaagt taaatatttc gatttcgaag agcgttccac agaacttcat
     3901 cgtcgcctaa gctcgatgag cagcatcggg gcgggcgcga agtgcgtgaa gcacggagtc
     3961 ggccttgcta cccaaggaga acttcttcgg atgcatagcg aagtggatca acgacaggtc
     4021 tgacgcgcaa atccgttaag ttcaatatcg ttttgtttac gcgaagaagc tgatgacaga
     4081 catgcaacgc tatggcgctc accggaccaa ctccggctga tgttcatcca cccacgtaaa
     4141 ccgcgagccg gtcaaacaac tcacgcttca cttacacaat ctttcttggc atcatggcgc
     4201 gaatgggaac tccgcgatca gcccgcatga cagcagcatc ctcccgagcc tggttctccg
     4261 cgtccattgc tgacttccta gaaacccaac ctgatgccat tgtcggacga ctggcgacaa
     4321 acaacgcctt cactctgctg ttaacacaga aggacgcgtg gttggcgcag atagggttcc
     4381 tccaagcaca tctccatggg ctaacgggct cgttgttttt ggagttcaac atcccacgca
     4441 tgggccgacg gattgataca gtcctactga taggtcctgt gctgtttgta ctcgagttca
     4501 aagtgggcga aagcgcgttc gaacgagcgt ccgtggatca agtgtgggat tatgctttgg
     4561 acctgaagaa ttttcatgaa gccagccatt ctgtttcgat tgttccaatt ctcatcgcca
     4621 ccggagctac gaaatcgacg ccgtctagtc tgcacgccga cgaggacaac gtgtatcgcc
     4681 cgattctggt gcaccccacc ggttttcgcg aagcgattga
//
LOCUS       DASUTG010000822         7836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1607421, whole genome shotgun sequence.
ACCESSION   DASUTG010000822 DASUTG010000000
VERSION     DASUTG010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607421"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF089_19125"
     CDS             complement(<1..658)
                     /locus_tag="VF089_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176129.1"
                     /translation="MEFYLFVSENGSKEESNGSVFYKVDNEELASSLCSLFTRDYGSE
                     DQEIKFWSTPTTEFDLEQKILMNETLDYLTEYRDPDPGIYDPMPSASILDRAQYAIRA
                     EKWEELLRAETTTAATSSDDTPFPSTNQSGAVPQMSEEDKRNSALILLMLFAQTYYRV
                     LELTISIATFATRLDLQLNESYFWLVRNIQNLLSNHEALKDFPAQFEPYFEDLYPSTI
                     A"
     gene            complement(696..1598)
                     /locus_tag="VF089_19130"
     CDS             complement(696..1598)
                     /locus_tag="VF089_19130"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX6176130.1"
                     /translation="MDAETELLVRRATPLIFGVFSQIENANGAREVRFGGSGIFVAPF
                     QTITARHVNRDLFRVDPDAADHLQRRLNDVERGDGRFYCELPHSSALFQARLGRRPRP
                     LIWHVRRSWDSVLTDISFMEVFAEGDEAAGAERDMAGFFEWSLLPPDRGAEVVMLGFP
                     QADIRTNAGVLNIDLNYVIQRGEVTDIYPIQRDRGMYNFPCFRINRPVDHGFSGGPVF
                     SEGRLCGIVSGGFDDDTYASSLWPLCLMEYEYPDLGGIGLKRSFSDLFRTGVLRSTAW
                     AGIEPRIEKRHDENGRPYAHLIDD"
     gene            complement(1688..2599)
                     /locus_tag="VF089_19135"
     CDS             complement(1688..2599)
                     /locus_tag="VF089_19135"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX6176131.1"
                     /translation="MLEGGATRLGDRARGCILGGAIGDAMGGPFEGQPGPIQFCEHCD
                     WNISDDTQLTLATCESIIELGRVSPQHIASRFVEWYRARRITGMGSSTLKALRDLDAG
                     SHWALAGARGEMSAGNGAAMRIAPLAFHLDPALDPDRQIIRDVCRITHHNEEAYVGGL
                     AIAIAIRSLAFAKASPTDLLEVVVQDLPDSRVRDRILALSAFSDEPSVPEVSAEFGSS
                     GYVVDSVPLALYAARSIVRIPFEDVLRSTIEAGGDTDTIASMTGQIAGAWLGHSRIPR
                     ELIDMLPNANYTERVSDEFARTLGTNA"
     gene            complement(2822..2896)
                     /locus_tag="VF089_19140"
     tRNA            complement(2822..2896)
                     /locus_tag="VF089_19140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2862..2864),aa:Val,seq:cac)
     gene            complement(3372..4343)
                     /gene="pfkA"
                     /locus_tag="VF089_19145"
     CDS             complement(3372..4343)
                     /gene="pfkA"
                     /locus_tag="VF089_19145"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6176132.1"
                     /translation="MRRIGVLTSGGDAPGMNAAVRAVVRSGVDKGWNVHGVRRGYAGL
                     IAGDFVQFGPRDVGGIIQQGGTILGTSRCEEFTTKAGRAKAIAALERQAIDALVVIGG
                     DGSQTGAYHLSQTGYPVVGIASTIDNDLFGSEISIGVDTALNVALEAIDRLKVTASSH
                     HRAFLVEVMGRNCGYLALMAGIAGGAEVIVIPEVELSPDQIACEFRNGYERGKRHAIA
                     VVAEGARYNAQRLADYFKENHERLGFDLRITRLGHVQRGGAPGAFDRLLATRFGVAAI
                     GQLAQGRGGVLIGLLKDEISVTPFSEILANKKPLDLRLLEMARILAR"
     gene            complement(4430..4837)
                     /locus_tag="VF089_19150"
                     /pseudo
     CDS             complement(4430..4837)
                     /locus_tag="VF089_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008277463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(4946..5371)
                     /locus_tag="VF089_19155"
     CDS             complement(4946..5371)
                     /locus_tag="VF089_19155"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6176133.1"
                     /translation="MTDIAEAANETSNTMTILLACIAGVSLLVGGIGIMNIMLVSVTE
                     RTREIGIRMAIGARSSAVRSQFLIESIVLSVTGGLIGIVLGIAVSLAIPKMLGWPTLV
                     STMAIIGSVIFSAAVGIFFGYYPARKAAALDPIEALRYE"
     gene            5619..6416
                     /locus_tag="VF089_19160"
     CDS             5619..6416
                     /locus_tag="VF089_19160"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6176134.1"
                     /translation="MFAVEQVWFESRDRTRIPMFLVHRREIELDGNNAAVIHGYGGFG
                     VSLLPAFAAHVIPFLERGGIYAIVNARGGGEFGEAWHRAGMREHKQNVFDDFIAAGEW
                     LIKEGYTRAAKLGCLGWSNGGLSVNAVAVQRPDLWKAVVAGAPVTDMARFHLAHGGRH
                     WIADYGSPEAPHDLGFLMQYSPYHTLPDKIEAPAILTIAPGNDDRVAPWHSYKMHAAW
                     QAASVSKSPILLRGEDQAGHHGSPEVTRTIDRYADIWAFFSWQLGLD"
     gene            6688..7050
                     /locus_tag="VF089_19165"
     CDS             6688..7050
                     /locus_tag="VF089_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176135.1"
                     /translation="MIEMLPLFVTNFGRAFDSIPLNVSKLIVYDQIHDSADIDDAAGL
                     EHVFQVVRNRLSGSPTHPYNIHEILVGHQKLDPGYRFQIKLPEIEQFEIFSVAINYYK
                     GRKLTAGATNTAQNNIWR"
BASE COUNT         1870 a         2110 c         2013 g         1843 t
ORIGIN      
        1 ccgcaatggt agaagggtag aggtcttcaa agtaaggttc aaattgagct ggaaagtctt
       61 ttagcgcctc gtggttgctc aagaggtttt ggatgtttct tactaaccaa aaataggatt
      121 cgttgagctg aagatctaaa cgagtagcaa aggttgctat gctgattgtc aactcaagga
      181 ctcgataata tgtttgagcg aacaacatta ggagaatcag ggcagagttt ctcttatctt
      241 cctctgacat ttgcggcact gctcctgatt gatttgtact tggaaatgga gtgtcgtcac
      301 ttgaggttgc tgcagttgtg gtctcagccc ttaacagttc ctcccatttt tcggcgcgaa
      361 tcgcgtattg cgctcggtct aatatgcttg ccgagggcat cggatcgtaa attccaggat
      421 caggatctcg atactcagtg agatagtcga gggtctcatt cattaggatt ttttgttcga
      481 gatcaaattc tgtggtgggt gtcgaccaga atttgatctc ttggtcctct gatccgtaat
      541 ccctagtgaa tagactacag agcgatgatg cgagttcctc attgtcgact ttgtaaaata
      601 cgcttccatt gctctcttct ttagacccgt tttctgatac aaacaaatag aattccattt
      661 attgccctcg attgtgcgga atgactacga gcgatctagt catcgatcag atgagcatac
      721 ggcctgccat tttcatcgtg acgtttctcg atcctcggct cgataccagc ccaggcagtt
      781 gagcgaagca cacccgtccg aaacaaatca ctaaacgacc tttttagtcc tatgccgcca
      841 agatctggat actcatactc cattaaacaa agcggccaca agcttgaagc atatgtatcg
      901 tcgtcaaagc cgccgctgac aatcccgcac aaccgacctt cggagaaaac gggaccaccg
      961 ctgaagccat ggtcaactgg cctattgatc cgaaagcacg ggaaattgta catcccccgg
     1021 tcgcgttgta tcggatagat atccgtgacc tcgccacgct gaattacgta gttcagatca
     1081 atgttcaaga ctccagcatt tgttcttatg tcggcctgag ggaagccaag cattactact
     1141 tccgcaccgc gatcaggcgg caatagagac cattcaaaaa aaccagccat gtctcgttca
     1201 gcacccgcgg cctcatcacc ctctgcaaaa acttccatga aggaaatgtc tgtgagcacg
     1261 gagtcccatg atcggcgaac gtgccatatt agcggtctcg gtcgtcttcc aagccttgct
     1321 tgaaacaaag ccgaagagtg gggtaactcg caatagaacc ggccatctcc tctttctaca
     1381 tcattcaacc gccgctgcaa atgatctgct gcatcagggt caactctaaa taaatctctg
     1441 ttaacgtgac gagcggttat ggtttggaat ggcgctacga aaatcccgga acctccaaag
     1501 cgcacctcac gcgccccatt cgcattctca atttggctga agacgccgaa gatcagaggc
     1561 gtcgcacgtc ttacaagcag ttcggtttct gcatccatag aagcgcccag caaaatatat
     1621 cttaatttag cgaggtctag acggcgcgca gtaatcttga gggatgctag tttcggaagc
     1681 ccccattcta agcgttcgtt ccgagagtgc gcgcaaattc atctgagacg cgttcagtgt
     1741 aattagcatt tggaagcata tcaatcaatt cgcgcgggat tctggagtgg cctagccatg
     1801 cgcccgcgat ttgaccagtc atcgaggcga tcgtgtcggt atcaccacct gcctcgatcg
     1861 tgctgcgaag tacatcctca aatggaatcc taacgatcga acgagctgcg tatagcgcca
     1921 atggcactga gtcaaccaca tatcccgatg aaccaaattc ggcggacact tctggaacgg
     1981 atggctcatc gctgaatgcg ctaagtgcca gaatgcgatc tcgaacacgc gagtctggta
     2041 agtcctgcac aactacctct aacaaatccg tcggagatgc cttggcgaat gcgagtgatc
     2101 gaatcgcgat cgctatagcg agtccgccca catatgcctc ttcgttgtga tgcgttatgc
     2161 gacaaacgtc gcggatgatt tgacgatcag ggtcaagcgc aggatcaaga tgaaatgcga
     2221 gcggtgcgat ccgcattgct gcgccgttcc cggcagacat ttctcctcgg gctccggcta
     2281 aagcccagtg cgaaccagcg tcgaggtcgc gaagtgcctt cagggtgctt gaacccattc
     2341 cggtgattcg gcgggctctg taccattcaa cgaagcgact tgctatgtgt tggggtgaga
     2401 cccgacccag ttcaatgatt gattcgcagg ttgcgagtgt tagctgagta tcgtctgaga
     2461 tattccaatc gcagtgctca cagaattgga tgggacctgg ttgtccttca aacggcccac
     2521 ccattgcatc gccgatagct ccgccaagga tgcagccacg agcgcgatca ccaagtctgg
     2581 ttgctccacc ttctagcatg aacgcccaat atacatccac ccggcacgaa ccacgtccgg
     2641 tcagacactc ccgtgctccc gatgtctcat gcgttcgctc ttacgatctt gaggctggag
     2701 cacgatctaa aaaagatcac ttttgttaga tttgatgacc gatcctgact tcagagtgtg
     2761 tgcgaaagtg tgtgcggagc aaaacgggcg gctgctcgcc gcccgtaagt tattgaaaac
     2821 atggtgcccg ctagtagatt cgaactactg acccctcgcg tgtgaagcga gtgctctacc
     2881 actgagccaa gcgggcgtaa atcccttaga atcaacgagt tacgtggact gctgggctgc
     2941 aagatttaac tgcgaatctc acccgaacgt cggcatgtta tatgatcgtg cccgcttgcg
     3001 ctagcggtag gggcactcgt ttagtttcag cttcccaagc tggtggtgcg ggttcgattc
     3061 ccgtcacccg ctccaaattc cttagtgatt cttaaactca agtttcaaaa gttagagaaa
     3121 ctttaaaacc gtcccgcaaa ccgtcccgca aatcctgttg gcttgagtgt cagcctctag
     3181 ggatgacact tgccaaggtt taaagaacgt tggactagca ttaccaagtc ttaccatcga
     3241 tacagctagt atttacgggt tcgagcgatc gtcaacgcat cgctttggca ggcttcgtat
     3301 caacacgtac cgccaccatc ttagccccat ttttacccct tcgagaaaga ttagccgacg
     3361 ctgggaagat ctcaccgcgc caggattctc gccatttcca gcagtctaag atcgagaggc
     3421 ttcttgtttg cgaggatttc actgaacggt gttacggata tttcatcctt gaggagccct
     3481 ataaggactc cgcctcgccc ttgagccaat tggccgatgg cagccacgcc gaaacgtgta
     3541 gccagcaacc gatcaaaggc tccgggcgcg cctcctcttt gaacatgacc gagccgggta
     3601 atgcgtaagt caaaccctaa tcgctcgtgg ttctccttga aataatccgc gagtctttgc
     3661 gcattgtatc gagcaccttc agcaaccacc gcgatggcgt gtcgctttcc acgttcgtat
     3721 ccatttcgaa attcgcaagc gatctggtca ggcgaaagct cgacttccgg tatgacgatt
     3781 acctcggcgc cgcccgcaat gccggccatc aatgccagat aaccacagtt gcggcccatc
     3841 acctcaacca ggaacgctcg atgatgtgaa gaggcagtta ccttgagccg gtcgatggct
     3901 tcgagcgcta cattcagtgc agtatccacg ccgatggaaa tttcagaacc gaataggtcg
     3961 ttatcaatcg ttgaagcaat gccgacaact ggataaccag tttgcgacag atgataggct
     4021 ccggtttgtg aaccgtcgcc gccaatgaca acgagagcat caatggcctg acgttctaaa
     4081 gcggctattg cttttgcccg accagctttg gtagtgaatt cctcacagcg tgacgtgcca
     4141 agtattgtgc cgccttgctg gatgatgccg cccacgtccc tagggccaaa ctgaacgaag
     4201 tctcccgcaa tcagtccggc ataaccacgg cgaactccgt gtacgttcca ccctttgtcg
     4261 accccggacc ggacgaccgc tctgacggca gcattcatgc cgggtgcatc tccgccgctg
     4321 gtcaagacac cgattcttct catatcgcct tgggtgagat cggggacagt ccgccgaaat
     4381 tggttacatt cgaagtcgcc caaagcggag gcgtgtgcga cagctcaact tcagccttgg
     4441 cgatcacgag ttgactcttg atggctgcgt caggattcag cgagatggaa cttatgccgc
     4501 gctcgaccag ccatcgagca aagtcgggat agtccgatgg tgcctgccct atcggcttgc
     4561 cggcgctctt tgcagcttcg attgcagccg caattatgtc gcgcaccgct tcatcacgtt
     4621 cgtcgaagag ataagcgacc gtaccggaat cgcgatccag accgactacc agctgcgtta
     4681 aatcgttcga tccaattgaa taaccatcga atacttgcaa gaagtcccgg gcgcgtacca
     4741 cgttcgaagg cagctcgcac atggcataga cctccaaatc atcttcgtgt tgcttgagtc
     4801 cgtgctgcgc catagttgcg atcacgcgtt taccctcggc gaccgcaaga ctgctgtccc
     4861 gagttccagc ggcaaaggcg tttccaacac tgcggcgcgg gcggcatggc cacggatggc
     4921 gagctcgtgg gcgcggtggc ctcgattact cgtatcgcaa ggcctcgatc ggatccaatg
     4981 cggcagcctt gcgcgccgga taataaccaa aaaatattcc cactgctgcc gaaaagataa
     5041 cggacccgat gattgccatc gtggatacca gagtcggcca gcccagcatc ttcgggatcg
     5101 cgagggacac ggcgattccc aggactatgc caatcaatcc gcctgtcaca ctgagcacga
     5161 tcgactcaat aaggaattgg ctgcgcacgg cggaagagcg cgctccgata gccatgcgta
     5221 tgccgatctc ccgggtacgt tccgttaccg acacgagcat gatgttcatg attccgatcc
     5281 cgcccaccag aagcgaaacg ccggcaatgc acgccagcag gatcgtcatc gtattgctgg
     5341 tttcgttcgc cgcctcggcg atgtctgtca tattgcggac tgtgaaatcg tttggttcac
     5401 tgggcgtcag gttgtgacgc tggcgcagta actccgtaat ctgcgattgg gccgtgtagg
     5461 tagcgtcctg cgagatggcg gaaacgtagg ctacttgcag tcgcgagctt cccaggattt
     5521 ttttctgaac ggtggtgtac ggggcgaaag caatatcatc ctgatcgact ctgacatatg
     5581 aacttcatct ccaggacgaa ggccgatttg acccggagat gtttgcggtc gaacaggtct
     5641 ggtttgaatc acgcgaccgg acgcgcatac cgatgttcct ggttcacaga agagagatcg
     5701 aactcgacgg aaacaacgct gcggtaatcc acggctatgg cggctttggt gtcagcctct
     5761 tgccggcgtt tgcggcgcac gtgattccat tcctggaacg gggaggaatc tatgcaatcg
     5821 tcaatgccag aggaggaggc gaatttggcg aagcatggca tcgtgccggt atgcgcgagc
     5881 ataagcagaa cgtcttcgat gacttcatcg cagccggtga gtggctgatt aaagaaggct
     5941 acacccgagc ggccaagctg ggttgtctcg gatggagcaa cggtggattg tcggtcaacg
     6001 ccgtcgcagt acagcggccc gatttatgga aggcagtggt tgccggcgcg ccggtcaccg
     6061 acatggcgcg ctttcatctg gcccacggcg ggcggcactg gatcgctgat tatggttcac
     6121 ccgaagcccc gcatgacctg ggcttcctga tgcaatacag cccgtaccac acgttgccgg
     6181 acaagatcga ggcacctgcg atacttacga tcgcccccgg caatgatgat cgcgttgctc
     6241 cctggcacag ctacaagatg cacgccgcct ggcaagccgc aagcgtttct aagagtccga
     6301 tcttactgcg cggagaagac caagccggac accatggcag tcccgaggtg acccgaacta
     6361 ttgaccgtta tgcggacatc tgggctttct tttcttggca acttgggctc gactgatgcc
     6421 actctttgag cgcgcgcacg cgactgttgg cgttgcggat ctcggcgcga tcaaccgata
     6481 aagctggccc gtaaactaac ccaagagcaa atggcgacgc agctaggacg gagcaagcga
     6541 tcgggaccac ggccgaacca tgtatccgaa tatgaacatg gcaagcgtca gccacgttcg
     6601 cgtcgaaata cgatcgcgga gaaaatctac aagcagattt ggtcagcaaa gcgtggcggt
     6661 gtggtcatgt cattgtggta ggggctaatg attgaaatgc tgccattatt tgtaactaat
     6721 ttcggtagag ctttcgattc aattcctctt aatgtatcta agctcattgt ttacgatcag
     6781 atacatgatt ctgccgacat agacgacgcc gccggcttgg agcatgtgtt tcaagtagtg
     6841 cggaacaggt tatccggatc accaacacat ccgtacaaca tccacgagat tttagttggc
     6901 catcagaaac ttgatcctgg ttatcgtttc cagatcaaac taccagagat tgagcaattc
     6961 gagattttca gcgtcgcaat aaactattac aagggccgta aacttacagc gggtgctacg
     7021 aataccgcgc aaaacaatat ctggcgttga cacgattacc gcacttgact attgtgcgcc
     7081 gcgttaatcc cagcgaggtg aatcctctga gcctgcttag tgcactctca ccgaaatcca
     7141 gcctattgcg ttagtcaatc atccatcgcc taccggatag tttttgatct ggcctcagga
     7201 cttctcccaa tagagctcaa ttgtcaatca cggtccggat ccttaccaca ctcaacaatc
     7261 tgtttccgag ggaatggtta tgagatcgag atcgaggccc gcgaagagca aaagtccagc
     7321 ctgaactgcc ggtaggtcta cacagaagtg gaagtattga ctcaatgact attgttgtca
     7381 tatcgtcgcg gctcatgaaa cgagtgggct ggtcctcaga gcctgcgaaa gtcgcactcc
     7441 tattggataa ctagccctaa atacctctat gacgacgatt gggtgacgca gtttttgtga
     7501 tttcacgaca aagtttgcga cttcacaccc gggcctgcat ctattcgggt ttggtgcgtc
     7561 caagtccgcg tgcgttactc aaaccagtga ggcacccaga tcattacgga tatcgcttaa
     7621 gacgacagtg aggctgttat catgcggctc aggaatggga acccgttaga ccttatatgt
     7681 gaggatggtg aacaagtcac cgtcactgtg caagcgaaag gtactaaacg gctagttgtc
     7741 tattctctcg acaacaatca aatagtaaac ctacaagagg atgcactcag attcaatttg
     7801 gacgccgcca aatctgggcc gacgatcttg acaatg
//
LOCUS       DASUTG010000823         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_867439, whole genome shotgun sequence.
ACCESSION   DASUTG010000823 DASUTG010000000
VERSION     DASUTG010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /gene="glgX"
                     /locus_tag="VF089_19170"
     CDS             complement(<1..2096)
                     /gene="glgX"
                     /locus_tag="VF089_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX6176136.1"
                     /translation="MKVWPGNNYPLGASYDGAGTNFSVFSEVAERVELCLFDEQGNET
                     RCDLPEVTSFCWHGYLPNVVPGQRYGFRVHGPWAPGEGIRCSPAKLLLDPYARAIEGQ
                     VDWNEAVFSYAFDDPDGPVNQADSAPFMPKSVVINPFFDWGNDRHPRTRLSETIVYEM
                     HVKGFSKKLEEIPEELRGTYAGLGHPKAIEYLTHLGINAVELMPVHQFVHDSHLLERG
                     LRNYWGYNSIGYLAPHNEYSSSGQTGQQVQEFKQMVKALHEAGIEVILDVVYNHTAEG
                     NHLGPTLSFKGLDNAAYYRLMNDDRRFYMDYTGTGNTLNMRHPHVLQLIMDSLRYWIV
                     EMHVDGFRFDLASTLARGLHDVDRLSAFFDIIQQDPVISQTKLIAEPWDVGEGGYQVG
                     NFPPLWLEWNGKYRDCVRDYWRGQDQTLSEFGFRITGSSDLYEGTGRRPYASVNFITA
                     HDGFTLNDLVSYNDKHNEANGEENRDGDSHNRSWNCGAEGPTDDPAVNELRKRQKRNF
                     LSTLFLSQGIPMLLGGDEIGRTQRGNNNGYCQDNEISWFDWQAADHDLLEFTTKIIEL
                     RKQHPVFRRRRWFQGRPIHGAAVTDLGWFTPEGIEMAEEHWGEGFAKAVGIFLNGDAI
                     GDPDPRGERIVDDSFYVLFNAHHEALRFVLPNENWGKDWTLILDTSGTQAEEPGPHKA
                     GDEVGVEARSLKVLRRV"
BASE COUNT          672 a          843 c          806 g          669 t
ORIGIN      
        1 acacgacgca gcaccttcag cgaccgcgcc tctacgccaa cttcatcgcc tgccttatgc
       61 ggccccggtt cttctgcttg tgtgccggag gtgtccaaaa tcaacgtcca atctttgccc
      121 caattttcat tcgggagaac aaagcgcaac gcttcatggt gagcattgaa gagcacgtaa
      181 aagctgtcat cgacgattcg ctcgccgcgc ggatccggat caccgatcgc atctccattg
      241 agaaaaatac ccaccgcttt ggcaaacccc tcgccccagt gttcctcggc catctcaatc
      301 ccctctggcg taaaccagcc gaggtccgta accgccgcgc cgtgaatcgg acggccctga
      361 aaccatcgac gtctacggaa caccggatgc tgtttgcgca gctcgattat tttagtcgtg
      421 aattcaagca gatcatggtc ggccgcctgc caatcgaacc aagagatctc gttgtcctga
      481 caatagccat tgttgttgcc cctttgagtg cggccaatct cgtcgccgcc gagcagcatg
      541 ggaatgcctt gagacaaaaa caaggtcgaa agaaaattgc gcttttgacg cttgcgcagc
      601 tcattgactg ccggatcgtc agtgggtccc tccgcgccgc agttccaaga acgattgtga
      661 ctgtcgccgt cgcggttttc ttcgccattg gcctcgttat gcttgtcgtt gtaggagacc
      721 agatcgttaa gcgtaaaacc atcatgggcc gtaatgaagt tcacgctcgc atacggccgc
      781 cggccggttc cctcgtacag atccgagctg ccggtgatgc ggaagccaaa ctcacttagc
      841 gtttgatctt ggccgcgcca ataatcgcgc acgcagtcgc ggtacttgcc gttccactcc
      901 agccacaatg gagggaagtt tcccacctgg taaccgcctt cgccgacatc ccacggttcg
      961 gcgataagct tggtctgact gatgactgga tcctgctgaa tgatgtcaaa gaaagccgaa
     1021 aggcggtcga cgtcgtgcag accgcgcgcc aaagtggacg ccagatcgaa gcgaaacccg
     1081 tcaacatgca tttcgacgat ccaatagcgc agcgaatcca tgatcagctg caacacgtgc
     1141 ggatggcgca tgttaagagt gttgccggtt cccgtgtaat ccatatagaa acggcgatcg
     1201 tcgttcatca ggcggtagta ggctgcattg tcgaggcctt tgaaggatag cgtcggcccc
     1261 agatgattgc cctcggcggt atgattgtaa accacatcga ggatcacctc gatgccggct
     1321 tcgtggagcg ccttgaccat ctgcttgaat tcctgaacct gctggccggt ctgcccgctc
     1381 gaggagtatt cgttgtgcgg cgccaaatag ccgatagagt tgtaacccca atagtttcgt
     1441 aagccccgct caagaagatg cgaatcgtga acaaactgat ggaccggcat cagctccact
     1501 gcgttgatgc ccaggtgcgt gagatattcg atcgctttgg ggtgtccgag accggcgtag
     1561 gttccgcgca actcttccgg gatttcctcg agctttttcg agaaaccttt gacgtgcatc
     1621 tcgtaaacga tcgtctcgct taaccgagtc cgcggatggc ggtcgttgcc ccaatcaaaa
     1681 aatggattga tcactaccga cttgggcatg aagggagcgc tgtccgcctg gttgaccggg
     1741 ccgtcgggat catcgaaagc atacgaaaaa accgcctcgt tccaatctac ttggccttca
     1801 attgctcgtg cgtagggatc gagcagcagc ttcgcggggc tacagcgaat gccttctccc
     1861 ggagcccagg gcccgtgaac ccggaagccg taacgttgac cgggaacaac gttaggcagg
     1921 tagccgtgcc agcaaaagct cgtcacttca ggaaggtcgc aacgtgtctc attcccctgt
     1981 tcgtcgaaca aacagagctc gactcgttcg gctacctcgg agaaaactga aaaattagtg
     2041 cccgcgccat cgtaggacgc gcctagagga taattattcc ccggccaaac cttcattaca
     2101 ctactccctg tgatgcttcg aacgatcagc cggagatgct ggagcggtct cgatgtggat
     2161 cagtcggcga tacttcgatt gttgaacacg tgtgaattca gcaaaataga tgccagcagg
     2221 ttcacaaccc gacgctgcat acggcagagc gactggcgct cgcgcacgaa tccgatagga
     2281 acatcatcaa tggtctttgt cagtggtatg agtacagttg gtaatggcct aggctggatc
     2341 gcgttgcgca aggaaactcg ggaccgattt tcgacttaat gcaaaggtcg gtgattaacc
     2401 ctggtgctgt gcgaatctgg aacatcggtt cttgataccc tatcccacga acgctacacg
     2461 agacattccg gaagcgagag attatgaatc gcgggaacaa agctcgaaaa cagcctctgg
     2521 caactcagag ctcgacgtga agtcggcttg gctgagctca gactcaaaga gtcgagcggc
     2581 tccgatgaga gcaactctct tactttccag atgctgggcg tgattcttgc acatcgcttc
     2641 gcctgcgggt tgtccggctt tagcaaacca tctgaatcac ttttcaccgc gcgctgcctt
     2701 gaagtaggag ctgcagaatc tcttcctcta gtatgggacc atctgcagag gagtggaccg
     2761 cgcctctctc acctggggtg tgcgatgcta tagcctgaat tgcagacgcg gtgcgcttgt
     2821 aaaaagcgtt tgccaccatc ggcggtgccg ctacttccct gtcggttcgg cgagcaagaa
     2881 agcgtgtaag attaaggtgc agccggaaac ggcttatcag cattcatcgt ggggaacctc
     2941 acgcgcgatc tgagcggcct cgcggttttt ctatggccaa gtcttatcga
//
LOCUS       DASUTG010000824         4097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1607587, whole genome shotgun sequence.
ACCESSION   DASUTG010000824 DASUTG010000000
VERSION     DASUTG010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607587"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF089_19175"
     CDS             complement(<1..680)
                     /locus_tag="VF089_19175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6176137.1"
                     /translation="MISLSLRGLTKKFGRVLAVDQANLDVHSGEFLVVVGASGCGKTT
                     TLRLIAGLESPDAGTIFIGGVPVNEIPVGKRNVQMIFQNFALWPHMRVYDENRYSNLS
                     LPLKVRKWSHDKIGEVLRPLARRVGIEESFFRRKPMELSGGQQQRVALGRAMATAPQV
                     LLMDEPLSNIDPPTRLRMRGEILKFHQENRLTTIYVTHNLADGIALADRIAIMRNGRF
                     EQVDTAEN"
     gene            804..1571
                     /locus_tag="VF089_19180"
     CDS             804..1571
                     /locus_tag="VF089_19180"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6176138.1"
                     /translation="MHGMLSPEELIADLQNALQQRHPKPHPIRQLLLSGRLTKEQLQW
                     WAKNQFYEFRNIHRFFGIRYQKCPVPELRRLLLENMVEEEGEDLFGGKYPSHRELWVQ
                     FAGGLGIPADEIMNYEPLPAIRAALEMYVSLVQQSHWAVAIGTGLVFEGGGPKRMKEE
                     REAIEKYYPWIPASTLDFFRAHEYHDEGHGDMVTNVIKQHCMEERLQGEMREAVKQRA
                     DIMWLQNDSIYNAFVRPSLAPQTIEEIEGPHGLKART"
     gene            1605..2651
                     /locus_tag="VF089_19185"
     CDS             1605..2651
                     /locus_tag="VF089_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX6176139.1"
                     /translation="MATKIIDLRSDTVTKPSSAMRRAMAEAEVGDDVFMEDPSVNRLQ
                     QRAAEIFGREAGLFVPSGSMGNLVCIMAQTQPGQEVICEATGHIYNYEMAAMAAIAGV
                     LPRVVNSSNGIMTWEQIFQAIRPKAYYRPQTSLVSLENTHNMAGGTVYPTRLAHAICD
                     HAHEAGLRVHLDGARVFNAAVHVGQSVAEITKKFDSVQFCLSKGLGAPVGSMIVGSSE
                     FIERCRSVRKMLGGGMRQAGVIAAAGLIALENGPKRLYIDHENAKLLAHGLAAIPGIR
                     IDPNYIQTNIVIYDIRETKLSSGEFLDRMAGHQVLGVPVDAERIRMVTHLDVDRSDVE
                     AAVTLIAEALRSHR"
     gene            complement(2673..3590)
                     /locus_tag="VF089_19190"
     CDS             complement(2673..3590)
                     /locus_tag="VF089_19190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176140.1"
                     /translation="MIIDCHAHLMPQSWFDAKSPKTVFDLDALFREQDEAGVDITVFG
                     NNWIRSPEGADPLRVVQEFNEHAAELTAKYPKRLLGLACSVPFDNGKILKETEKAIRD
                     YNLKGIMINTSVDGEYLDSPRATPFFELASELEVPLFVHPPKFTIGNEKMEIFRLPEM
                     LGRPFDTTLALTRFIFTGGFERFPKLKMICAHVGGALPMLPGRYGVGYELRKDMSFGP
                     WEPDVMTRPPASYISQLYVDTVSYHPAAVQCAIDTLGIDHVVFGSDSPPVPLPLKRAV
                     DTVQDLRISDADKQKILGGTAAKLLGLAF"
     gene            complement(3685..4002)
                     /gene="sugE"
                     /locus_tag="VF089_19195"
     CDS             complement(3685..4002)
                     /gene="sugE"
                     /locus_tag="VF089_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX6176141.1"
                     /translation="MPWINLAIAGILEVFWAISLKYTEGFSKFWPNLFTVFGMIASFY
                     FLAQALKVIPVGTGYAIWTGIGAAGTAIFGIILFDESAAPARLACITVIVAGIIGLKL
                     TSS"
BASE COUNT         1002 a         1044 c         1136 g          915 t
ORIGIN      
        1 aaattttcgg ccgtatcaac ttgttcgaac ctaccgtttc tcatgatcgc gattcgatcc
       61 gccaaagcga ttccatcggc tagattatgt gtgacataaa tcgtcgtcag cctgttttcc
      121 tgatgaaatt tcaagatttc cccgcgcatt cgcagccggg tgggaggatc gatattgctc
      181 aatggttcat ccatgagaag aacttgcggt gctgtcgcca tagcccgtcc cagggcaacc
      241 cgctgctgct gcccgccgga cagctccatc ggcttgcgcc tgaaaaaact ttcttcgatg
      301 ccgacgcgcc gcgccagggg acgcagcacc tcgccgatct tatcatggga ccacttacgc
      361 actttgagcg gtagcgacaa attgctgtag cggttttcat catagactct catgtgcggc
      421 cacagggcga aattttgaaa gatcatctgc acgttgcgtt tgccgacggg aatctcatta
      481 accggcacgc cgccgatgaa gatggtgccg gcatcaggtg attcgagtcc ggcgataagt
      541 ctaagggtcg tggtcttacc gcagccgctc gcgccgacca cgacgagaaa ctcaccgcta
      601 tgaacgtcga ggtttgcttg gtcaacggct agaactcgac caaacttttt cgtaaggccg
      661 cgtaatgata gtgagatcac aggacctcca tgctagtaaa cgccgtcgca aaaaacaatg
      721 acaaacccgg tggcggcgta ccgacgactt gtctatgatg catccaatcg aaggcgattt
      781 cttatcctgg aaggagattt cttatgcacg gaatgctttc acctgaggag ctgatcgcgg
      841 accttcaaaa tgccttgcag cagcgccatc ccaaacccca cccaattcgt cagctgctct
      901 tgagcgggcg tctaaccaag gagcagcttc aatggtgggc gaaaaatcag ttttatgagt
      961 ttcgcaatat tcaccgcttc ttcggcattc gttatcagaa gtgtccggtt ccggagttac
     1021 gccggctgtt attggaaaac atggtggaag aagaaggaga agatttgttc ggcggcaaat
     1081 atccgagcca tcgcgagctc tgggtgcaat tcgccggcgg tttgggcatt ccggcagatg
     1141 agatcatgaa ctacgagcct ttgccggcga ttcgcgcggc cttagagatg tatgtctctc
     1201 tcgtgcagca gagtcattgg gccgtggcga tcggaacagg actggttttc gagggaggcg
     1261 ggccgaaacg catgaaagag gagcgtgagg cgatagagaa atattatcct tggattccgg
     1321 caagcactct ggattttttc cgcgctcacg aataccacga tgaaggtcac ggcgacatgg
     1381 taactaacgt gatcaagcaa cactgtatgg aggagagact gcaaggtgaa atgcgcgaag
     1441 ccgtcaagca acgggccgac atcatgtggt tgcagaacga tagcatctac aacgcttttg
     1501 tccggccgtc gttggcgccg caaacaatcg aagaaatcga aggaccccat gggttaaaag
     1561 cgcgaacttg agctcgccga taaactcaag ggtcgaaggt cggaatggct actaaaataa
     1621 tcgatttgcg gagcgatacg gtaactaagc catcatcggc aatgcgccgg gcgatggctg
     1681 aggcggaagt cggcgatgac gtgtttatgg aggatccgag tgtcaatcgt ttgcagcagc
     1741 gcgcggccga aatttttgga cgcgaagcgg gattattcgt cccatcaggc tcaatgggta
     1801 acctcgtctg tatcatggca cagactcaac ctggacagga agtgatctgc gaagcgaccg
     1861 ggcatatcta caactacgag atggccgcta tggcagcgat tgccggcgtt ctccctcggg
     1921 tggtcaactc cagcaacggg attatgacct gggaacagat atttcaggcg attagaccga
     1981 aggcttatta tcgtccgcaa accagcttgg tgtctctgga gaacacccac aatatggccg
     2041 ggggcacagt ttatccgact cgtttggcgc acgccatctg cgatcatgct cacgaggccg
     2101 ggttaagagt tcatctcgac ggcgcgcggg tcttcaacgc ggcggttcac gtcggccaga
     2161 gcgtggcaga gattaccaag aaattcgatt cggtgcagtt ctgcttgagc aagggattgg
     2221 gtgcgccggt gggttcgatg atcgtcggct caagcgagtt tatcgaacgc tgccgttcgg
     2281 tccgcaaaat gcttggcggc ggcatgcgcc aggccggcgt catcgctgca gcagggttga
     2341 ttgcgttgga gaatggaccc aagcgcctct acatagatca cgaaaacgcc aagttacttg
     2401 cgcatggact cgccgcaatc cccggcatac ggatcgatcc gaattatatt cagacgaata
     2461 ttgtgatcta cgatataagg gaaaccaaac taagctccgg ggagtttctc gacaggatgg
     2521 ccgggcacca ggttttggga gtgccggttg atgcggagcg gatacggatg gtgacccatt
     2581 tggacgtcga tcgatcggat gtcgaggcgg cagtaaccct catcgccgaa gctttacgtt
     2641 ctcaccgcta aaaattcgct ggcgcgagcc cactagaacg cgagcccaag aagcttggcc
     2701 gccgtcccgc cgaggatttt ttgtttgtcg gcatctgaaa tacgcaggtc ctgcactgtg
     2761 tcaacggctc ttttaagcgg aagcggcaca ggcggagaat cgctgccgaa aacgacgtga
     2821 tcaatgccca gggtatcgat tgcgcattgg accgccgccg ggtgatagga gactgtgtct
     2881 acataaagtt gactgatata gctcgccggt ggtcgggtca tcacgtcagg ttcccacgga
     2941 ccgaagctca tgtctttgcg caattcatag ccgacaccat aacggcctgg cagcatcggt
     3001 agagctccac caacatgggc gcagatcatc tttagcttcg gaaatcgttc gaatccgccg
     3061 gtgaagatga atcgtgttag agcaagcgtg gtgtcgaaag gtcgaccgag catctctgga
     3121 aggcgaaaaa tctccatttt ttcattaccg atggtgaact ttggcggatg aacaaaaagc
     3181 ggaacttcaa gctcgctcgc caattcaaag aacggtgtgg cgcgcggcga gtcgaggtat
     3241 tcgccatcga cgctcgtgtt gatcataatt cctttgagat tatagtcacg gatcgccttt
     3301 tcggtttcct tcaggatttt gccattgtca aatgggacgg aacaggccag accgagcagt
     3361 cgtttggggt atttcgccgt taactcagcg gcatgctcgt tgaattcttg cacaacgcgg
     3421 agcggatcgg cgccttcggg tgagcgtatc caattgttac cgaatactgt aatatctacg
     3481 ccagcttcgt cctgctctct aaaaagcgcg tcgagatcga aaaccgtctt cggggacttg
     3541 gcatcaaacc aactctgcgg catcagatgc gcatgacagt caatgatcat aatggattga
     3601 ccctttccga gagaaaagta ttcagagagc cttactgact ttctaagatc aggccgacat
     3661 agtcaaccga tttccggcga cacattacga ggaagtaagt ttcaggccga tgatgccggc
     3721 aacaatcact gtgatgcacg ccaggcgcgc tggggcagcg gattcgtcga agagaatgat
     3781 tccaaagata gccgtcccgg cggctccgat gccggtccaa atggcgtatc cagtgccgac
     3841 aggaattacc ttcagagcct gggcaagaaa gtagaaactg gcaatcatcc caaagacggt
     3901 aaagagattg ggccagaact tactaaagcc ttcggtatat ttaaggctga tagcccaaaa
     3961 aacttccaga atcccagcta tcgccagatt gatccaaggc atacttaccc ctaacgcagt
     4021 tcctcagcgg tagacccagt gtgatcgaga cgattacaaa ttatggtaaa ttcgcgaggc
     4081 acacaacttt cgaatca
//
LOCUS       DASUTG010000825         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1162980, whole genome shotgun sequence.
ACCESSION   DASUTG010000825 DASUTG010000000
VERSION     DASUTG010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162980"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF089_19200"
     CDS             complement(<1..458)
                     /locus_tag="VF089_19200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176142.1"
                     /translation="MTTGKSASIWTRAFALLCLAQFLGYAQHFMLAPVLPLYVTDLGG
                     SAFLVGLVLASFAVTSVVIRPLIGHWADRWSETGVLIGGLLFQGASVFLCLLPMIEAV
                     MLANALRGLGWAGLNTGGYSLLALTAPESRRGEASGLYSGVQGAASIIFPA"
     gene            747..1442
                     /locus_tag="VF089_19205"
     CDS             747..1442
                     /locus_tag="VF089_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6176143.1"
                     /translation="MAEKRSAAKEFHFVVSETAGVVSGLLLKPEHSVSLLVFAHGAGA
                     GMRHRFIEDLSQKLAELGVATMRYQFPYMEKRVKRPDSEGVLIATVEAALTAAEKYSD
                     GVPIFAGGKSMGGRMTSLALAKAPLNKVHGLIFFGFPLHAPGAPSAERGKHLADVQAP
                     MLFIQGSRDALADLKLLKPLCAELDGRAELFVVDGGDHSLHMSKRSGRTDNEVLDEVA
                     AKTSSWMAAKAVE"
     gene            1524..2012
                     /locus_tag="VF089_19210"
     CDS             1524..2012
                     /locus_tag="VF089_19210"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6176144.1"
                     /translation="MFLNEQEALHALKGPDPLSAVNAEAFLWRYWCQSGDPEVDRVFR
                     DGVDAMQRGELELAETSFDKVIEMARQFAEGWNKRATVRYLRQNFAGSIRDCQETLNR
                     NPNHFGASAGQGLCHMALDEYREAAICFRRALEIYPHLKAVQQNLTLAQSQASVTEGY
                     LH"
     gene            2096..2653
                     /locus_tag="VF089_19215"
     CDS             2096..2653
                     /locus_tag="VF089_19215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX6176145.1"
                     /translation="MSIRVAGIAGSLREGSFNKALLRAAVELAPAAMEIKIYTGLGDI
                     PPYNEDVFAKGDPEPVAVMKAAISQADAILISTPEYNYGVPGVLKNAIDWASRPAGKS
                     VLNRKPAAIMGCSPGLGGTIRVQHALRQTFVFTETYVMLQPEIKIPSAAPLFDPSVRL
                     NDENTRQHLKKFLEAFVVWIDRFKS"
     gene            3027..>3697
                     /locus_tag="VF089_19220"
     CDS             3027..>3697
                     /locus_tag="VF089_19220"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176146.1"
                     /translation="MKKLFLALTLVVWSVAAAHAQAPFFQGKTITTVVGYLPGDGYDI
                     WARLLAAHMPKHIPGNPGMIVQNMPGAGSMIAANYVYNVAKPDGTTIGSIGPSLYFDQ
                     LVGKKEVSFDWAKFGWIGSTENTPWLLYMKADTPFKSIEDIRKATQAPKCSATSTGTS
                     GHFIPKLLEEAFGAKFDMVMGYKGGAEQDLAFERGEVVCRSLSIPTFFAREPFITWRK
                     KNVVR"
BASE COUNT          856 a          944 c         1056 g          841 t
ORIGIN      
        1 gcgggaaata ttatcgatgc ggcgccctgg acgccgctgt aaagacccga tgcctcgccg
       61 cgcctagact cgggggctgt cagcgccaat agtgagtaac cgccggtgtt gagaccggcc
      121 catcccaagc cgcgcagcgc attggctaac atcaccgctt caatcatggg gagcaagcag
      181 agaaatacgc tggccccttg aaatagcagt cccccgatga gcacgcccgt ctcgctccag
      241 cgatccgccc agtggccgat aagagggcga atgacaacgc tggtaaccgc gaaagaagcc
      301 aacacgagtc caacaaggaa cgccgagccg ccgagatcgg tcacgtaaag cggcaatacc
      361 ggcgccaaca tgaaatgttg cgcatacccc aggaactgag ccagacagag tagcgcaaaa
      421 gcgcgcgtcc atatggacgc ggattttccg gtagtcatgg aattgaaaac tctaagtcga
      481 tttttgctcg cgtaatggca aagtgcggcg tgattacaag aacctgtaag ctatcacgat
      541 caacacgttt gagcgagatc aagcaggatt gctcgggatc gcacttcgtc tagcaactac
      601 gcttgcattt ttctggccga tcttagtggc tcgggtagtg cgcacgtcgt tatcgttgcc
      661 gtggtgcggc tttcgttata gtttccgaaa ggaacgagca aacagtagca ggcgctcgaa
      721 atttcaaaac aaagtggagg gtagggatgg ctgaaaagcg atccgcggct aaagaatttc
      781 attttgtcgt aagcgagacc gccggggttg tatcgggact tctgttaaaa cctgagcact
      841 ctgtgtcgtt gctggtattt gcgcatggcg cgggagccgg aatgcgtcat cgctttatcg
      901 aagatctctc gcaaaaattg gctgaactcg gcgtcgctac catgcgttac cagtttccct
      961 atatggaaaa acgagttaaa cgacccgatt ccgaaggagt tctcatcgca acggttgaag
     1021 ccgcgctcac tgcggctgag aagtattccg atggcgtacc gatctttgcc ggcggcaaat
     1081 caatgggcgg tcgaatgact tctctcgcgc tagcaaaagc gccgctaaat aaggtgcatg
     1141 gcctgatctt ttttggcttc ccgctgcatg cgccgggagc gccgtcggcg gaacggggca
     1201 agcatttagc tgatgtccaa gcgccgatgc tttttattca gggttcgcgc gacgctttgg
     1261 ccgatctcaa actgctaaag ccgctctgcg ccgagttgga tggacgtgcc gagcttttcg
     1321 tggtcgatgg cggcgatcac tcgcttcata tgtcgaagcg ttccggtcgc acggataatg
     1381 aagtgcttga cgaagtcgca gcaaagactt catcttggat ggccgccaaa gcggttgaat
     1441 gatctggacg ggctatcttt caccttgatt atcggatgtc ataaccaaag agagatctcg
     1501 ccgttgatct gagaggttgt gtcatgtttt taaacgaaca agaagcgctg cacgctctta
     1561 aagggccgga cccgctctcg gccgttaatg cggaggcatt tctttggcgt tactggtgtc
     1621 aatcgggcga cccggaagtg gatcgcgttt tccgtgacgg tgtcgatgcc atgcaacgag
     1681 gcgaactcga gttggcagag acttcgttcg acaaggtgat cgagatggcg cggcagtttg
     1741 cagagggatg gaacaagcgc gcaactgtgc gctacttgag gcagaatttc gccggatcga
     1801 ttagggattg tcaggaaacc ttaaatcgaa accctaatca cttcggcgcc tcagccggcc
     1861 aggggctgtg ccatatggcg ctggatgaat accgcgaagc agccatctgt tttcgccgcg
     1921 cgctggaaat ttatccgcac ctcaaggcgg tgcagcagaa ccttactctg gcgcaatccc
     1981 aagcatccgt gactgaaggg tatcttcact gatgtaaact gatctaccct aaggcgtttt
     2041 aacctttgat tgcgccgatg agattacata aaaaaagcat aatgatggag gttgaatgag
     2101 cattcgagtc gccggaatag caggtagtct ccgagaggga tcttttaaca aggctctgct
     2161 gcgggctgct gtagagcttg cgccggcagc aatggagatc aagatttata ccggcttagg
     2221 cgatatcccg ccctataacg aggacgtttt tgctaaggga gatcccgaac cagtggcggt
     2281 gatgaaagcc gccattagtc aggctgatgc aattctgatc tccactcctg aatacaacta
     2341 tggtgtgccg ggcgtgctca agaatgccat cgactgggcc tcgcgcccgg ccggcaagtc
     2401 tgtgctgaat cgtaagcccg cggctatcat gggttgttcc cccggactgg gcggaactat
     2461 acgagtgcaa cacgctttac ggcagacgtt tgtcttcacg gagacctacg tcatgcttca
     2521 gccggagatt aagattcctt cagctgcgcc gctttttgat ccatccgtcc gattgaacga
     2581 cgagaacacg cgccagcacc tgaagaaatt ccttgaagcg ttcgttgttt ggattgaccg
     2641 gttcaagtct tgataggcaa gagatccgtg cgcgtcgctt cttgtgttcc gttcgaagtt
     2701 gcagtcacgt gcacgaccta gaggcgtgat ctacttcgga ggttgcgcgc gaagacctga
     2761 ttcgcaatgc caaaggatta tcggtcatcc gaaaactaag atagttcccc tacagcaaat
     2821 attctttgca caacggtcgc gcggggtcgg gtcggaagtc cggggctcgt gggtccgctg
     2881 tattgagatt aacgacattg aacctcaacc gttagtcggc tatagctggt tttcgtggga
     2941 agatcgaacg tccggcggat aatcgtagaa gctgatcatt cccttgattc taagcacgtc
     3001 gcgcggtcga aaatagaaag gcactcatga agaaactttt cctcgcttta acgctcgttg
     3061 tctggagtgt agcggcggca cacgcacagg cgcctttttt ccagggaaaa accatcacga
     3121 cagttgttgg ctacctgcct ggtgacgggt atgacatttg ggcgcgcctg ctggcggcgc
     3181 atatgccgaa acacattcct ggtaatcccg gcatgatcgt gcagaacatg cccggcgccg
     3241 gctccatgat cgcggccaat tacgtctaca acgtggccaa gccggatggg acgacgatag
     3301 gctccatcgg tccctcgctg tatttcgatc agcttgtcgg caaaaaggaa gtgagtttcg
     3361 actgggcaaa gttcggttgg atcggttcca ctgagaacac gccgtggctg ctgtatatga
     3421 aagccgacac accgtttaag agcattgaag acattcgcaa ggccacccag gctccgaaat
     3481 gttcagctac gagcacaggg accagcggtc atttcattcc caagctgctg gaggaggcct
     3541 tcggtgccaa gttcgatatg gtaatgggct ataaaggcgg cgcggagcag gacttggcgt
     3601 tcgagcgggg cgaggtcgtt tgccgttcgc tgagcattcc aacctttttt gcccgtgaac
     3661 catttatcac ctggcgcaag aagaatgtgg ttcgaat
//
LOCUS       DASUTG010000826         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_128269, whole genome shotgun sequence.
ACCESSION   DASUTG010000826 DASUTG010000000
VERSION     DASUTG010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128269"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VF089_19225"
     CDS             <1..676
                     /locus_tag="VF089_19225"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6176147.1"
                     /translation="APASAEKFPAAFKDKEGLVTAWRHTPIGVLYNTDSVPAADAPKS
                     LDDLLHPKWKGKIVLPDPTRHTSTAQFLASLRKIKGEGWIDYVKALAKQSPLLIESFA
                     PVPTDILRGEAMLGFTYLQYVYQNKGPLGYVLMDKILTDTNDLGLSAKAMNPNAAKLY
                     MEYLCSAEGQKLVADLGEFVLSPGIYPPIKDADKITANSIFMDNPSADEYKKLSAEFR
                     QIFFGK"
     gene            complement(875..1810)
                     /locus_tag="VF089_19230"
     CDS             complement(875..1810)
                     /locus_tag="VF089_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6176148.1"
                     /translation="MSNEELPSSYKYVALPDRRPLRFPNGARLAIILTINIEYWEKSR
                     PGQKEPLFTGGPMTIPHALPGDVLDTANWTWREYGQRIGIWRLIDLFDKSGVKPSCTI
                     NGLTMVERRRIVDAVKERGWELVPHNWAQNDLLTSYANQPDEERAVIKRTLDKYQEVV
                     GRPAKAWLSSAIRGTLYTPAFLREFDLIAYCDYLNDDQPYLIQTLHGPIVCVPYSNDV
                     NDFNLFARGGMSTSAGLETLKMCFDQLYEESHETGRIMNFGLHPHVMGQPFRVRALRE
                     FIEYAASHKNVWFTSREAIATWYLENHESHIPTEP"
     gene            complement(2028..3011)
                     /locus_tag="VF089_19235"
     CDS             complement(2028..3011)
                     /locus_tag="VF089_19235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176149.1"
                     /translation="MNIDFHAHVYPEDYLKKLEASTGDVRIETDNKGEKWILSMGAKA
                     GPITPDFFDLDVRVDRITETRVDMQILSSPHPGVDRFSPEESAEMSRVINNGIAKAVR
                     QYPKRFQGIAMLPLIDTRLALRELDRAILELGLKGMCMLTNVAGKTPDSDFLLPVYER
                     AQQLGVPIFIHPTTPLGAQVMQEWRLAIILGFEFDIVLSATRLAYAGVLDRFPELRFV
                     ISHLGAGIPFLAGRIDRGYHDPTCGIKTKKPTSEYLRELYCDTVSFYQPALAMAYEFY
                     GASRMVLGSDFPLIIGDLPAAVPSIEAMPIPNREKEKILGENVKQLLKLDI"
     gene            complement(3075..>3972)
                     /locus_tag="VF089_19240"
     CDS             complement(3075..>3972)
                     /locus_tag="VF089_19240"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6176150.1"
                     /translation="TLAPYADELYVFPSPSRTQQSDAPRFAFAFTVPVATPGLKFICR
                     DSLDTGGSPADYPLASRYEEMDAVAVFHNVVVPWERVFLKGDIVLCNRLFRDTPAFLH
                     GIHQFTVKNLAKAEFVLGIASLMAEAIGRTELPAYQQMLGEIVDAVETERAYLRAAEA
                     DAITDESGFVHPNPNILSTARNYFPKIYPRLIEILQLIGSSGLMATVSKRDLDSPEVG
                     EEINRYYQSATLMGRERVQLFRLAWDLACSGFAGRQVLYERFFAGDPFLLMASRFLSY
                     DRSAAVARVKALLARSYNDTEK"
BASE COUNT          878 a         1066 c         1118 g          910 t
ORIGIN      
        1 cgcgccggcg tcggctgaaa agtttcccgc tgccttcaag gacaaagagg gattagtcac
       61 tgcctggcgg cacacgccga ttggtgtgct ttacaatacg gattcggttc cggcagcgga
      121 tgcgccgaaa agcctcgacg atcttttgca cccgaaatgg aagggaaaga tcgtccttcc
      181 cgacccgacg cgccatacca gtactgcgca atttctcgcc agcctgcgga agatcaaggg
      241 tgaaggctgg atcgattatg ttaaggcgtt agccaagcag tcgccgctgc tcatagaatc
      301 atttgccccg gtgcccaccg atatcctccg tggcgaggcg atgctcggat ttacctatct
      361 tcagtacgtc taccagaaca aaggcccgtt gggttacgtt ctgatggaca agatcctcac
      421 cgacaccaac gatctcggtt tgagcgccaa ggcgatgaat cccaatgccg ccaaactcta
      481 catggaatat ctctgctccg cggaagggca gaagctggtg gccgatctcg gcgagttcgt
      541 gttgtcgcct ggtatctatc cgccgatcaa ggacgccgac aaaatcaccg ccaatagcat
      601 cttcatggat aatccgtccg ccgatgaata caaaaagctg agcgccgaat tccgccagat
      661 ctttttcggg aagtagagta tagcgagaga acgacgtagc gttcttctag tccaatcctc
      721 gatttctatg cctatttgct gttcctgccc gtatgttttg cctggctaat tcccttgtca
      781 aaattcttgg gttatcgcgt agtatcggcg aaattcaatt tacactgagt aaagcgactg
      841 aacttagcag ttttccctac ggtgctcgaa ttgatcacgg ttcggtggga atgtgtgact
      901 catgattttc taaataccag gtcgcaatcg cctcccggct ggtgaaccaa acgtttttgt
      961 gtgaagcggc atattcgatg aactcgcgca atgcgcggac tcgaaacggc tggcccatga
     1021 catgaggatg taatccgaag ttcatgatgc ggccggtctc gtggctttct tcatagagct
     1081 ggtcgaagca cattttcagg gtctcgaggc cggcagaagt cgacatcccg ccgcgcgcga
     1141 acagattgaa gtcgttgaca tcgttggaat agggcacgca aacaatcggt ccatgcaatg
     1201 tctgaatcaa gtagggctga tcgtcgttca aataatcgca ataagcgatc aggtcaaatt
     1261 ctctgagaaa cgccggtgtg tacaaagtgc cccggatcgc tgaagagagc catgccttgg
     1321 ccggccggcc gaccacttcc tggtatttat cgagcgtgcg tttaatgacg gcacgctctt
     1381 catccggctg atttgcgtaa gacgtaagca gatcgttttg cgcccagttg tgcggcacca
     1441 gttcccagcc gcgctctttt accgcatcga cgatgcgccg ccgctctacc atggtcaggc
     1501 cgttgatcgt gcacgatggt ttgactccgc tcttgtcaaa caaatcgatt agccgccaga
     1561 tgccgatgcg ctggccgtat tcgcgccatg tccaatttgc cgtatcgaga acatcgccgg
     1621 gcagcgcgtg cggtatcgtc atcggaccgc ccgtaaacag cggctccttc tgacccggac
     1681 gggatttctc ccagtattcg atattgatcg tcaggatgat cgcgagtctt gcgccattag
     1741 ggaagcgtaa gggcctgcgg tcgggcagcg ccacatactt ataagaagac ggcagttctt
     1801 cgttgctcat acgacatccg gcaagcggat tggttacaaa aacactccgt cccggcggac
     1861 agccaatctt ggccgcactt ttaatatacg agttgcgtct tttgagatcg gctcgacttc
     1921 tgaagtgtcg ctgtccgaag cttgtcctgt ccccttcgcc aaatcttctt tgccattacc
     1981 gccggaaaat gcattacaaa caaaatacgc cgtcctttcg cgaaagttca gatgtccagt
     2041 ttgagcagtt gcttaacgtt ctcgccaagg atcttttctt tctcgcgatt ggggatcggc
     2101 atagcttcga tactgggaac agcagcgggg aggtcgccga tgatcagtgg gaagtcgctg
     2161 ccgaggacca tgcggcttgc gccgtagaac tcgtaggcca ttgcgagcgc cggttgataa
     2221 aacgacactg tgtcgcagta gagctcgcgc aggtactcgg aagtcggttt cttggttttg
     2281 atgccgcagg tgggatcgtg gtagccgcga tcgatccggc ctgccagaaa tggaatgccg
     2341 gcgccgaggt gggagatgac aaacctaagt tcgggaaagc ggtcgagaac tccggcgtag
     2401 gcaagccgcg tcgccgagag cacgatgtcg aattcgaagc cgaggatgat cgcgagccgc
     2461 cactcctgca tgacttgcgc gcccaggggc gtagtcggat ggatgaagat tggaacgccg
     2521 agctgttgcg cgcgctcgta aaccggcagg agaaaatccg agtcgggagt tttaccagcg
     2581 acgttggtga gcatgcacat gcctttgagg ccgagttcga ggatcgcgcg gtcgagttcc
     2641 ctcaaggcga gcctcgtgtc gatcagcggc agcatggcga taccctggaa gcgctttgga
     2701 tattggcgca cggctttggc aatgccgttg ttgatcacgc ggctcatttc ggcgctttct
     2761 tccggtgaga aacggtccac gcccggatgg ggcgacgata gaatttgcat gtcgacgcga
     2821 gtttcagtga ttcggtcgac ccggacgtcg aggtcgaaga aatcaggcgt gatcgggccg
     2881 gctttggcgc ccatgctcaa gatccacttt tcgcccttgt tgtcagtttc gatcctgaca
     2941 tcccccgtcg aagcttcaag tttttttaaa tagtcctccg gatagacatg ggcgtgaaag
     3001 tctatgttca tgtttgaatc tcctctgaaa ctcgcaaata gtttccgatg gattcggtga
     3061 tcgaatgcgt tccattattt ttcagtgtcg ttataggatc gcgccaacaa ggctttgacc
     3121 cgggcgacag cggcgcttcg atcgtagctt aagaagcggg atgccatcag caggaacgga
     3181 tctccggcga aaaagcgctc gtagagcacc tgacgtccgg cgaagccgct gcaggccaga
     3241 tcccaagcga ggcgaaagag ctgtacgcgt tccctgccca ttaaagttgc cgattgataa
     3301 tagcggttga tctcttcgcc aacctcgggt gagtccaagt cgcgtttgct gactgtcgcc
     3361 atcaagccgc tcgagccgat aagctgcaag atttcgatca gtctcggata aatcttaggg
     3421 aaatagtttc gcgcggtgga cagaatgttc gggttcggat ggacgaagcc gctttcgtcg
     3481 gtgattgcgt cggcttcggc cgcgcgcaaa taggcacgct ccgtttcaac ggcatcaacg
     3541 atctcgccta gcatttgttg atacgcgggc agctccgtcc ggccaattgc ttcggccatt
     3601 agactcgcga tgccgagaac gaactccgcc ttagcgagat ttttgaccgt aaattgatga
     3661 atcccgtgga gaaaagccgg cgtatcgcgg aataagcgat tgcagagcac gatatcaccc
     3721 ttcaaaaaaa cccgctccca gggcaccacg acattgtgga acaccgcaac ggcatccatc
     3781 tcttcatagc gcgatgcaag cgggtagtcc gccggcgacc ctccggtgtc gagactatcg
     3841 cgacaaatga atttcaatcc aggcgtcgcc accggcaccg tgaaggcaaa ggcaaagcgg
     3901 ggcgcgtcac tttgttgagt acgcgacggc gaagggaaca cgtagagctc gtcggcgtac
     3961 ggcgcgagcg tt
//
LOCUS       DASUTG010000827         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1757938, whole genome shotgun sequence.
ACCESSION   DASUTG010000827 DASUTG010000000
VERSION     DASUTG010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757938"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..2669
                     /gene="treY"
                     /locus_tag="VF089_19245"
     CDS             12..2669
                     /gene="treY"
                     /locus_tag="VF089_19245"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX6176151.1"
                     /translation="MSPELRATYRIQLHRGFAFADAAAITDYLAELGISHLYCSPYLQ
                     AALGSTHGYDVVNPQQVNQELGGINGHARLCAALEENGLKQVLDIVPNHMAIGGRENP
                     WWWDVLENGPSSRYAGFFDVDWDPPEAKLRNTVLLPVLGDHYGRVLEKGEVALEREGG
                     QFIFRYHDHIFPVSPRSLDELIANAAGRCASDDLAFIAEALRQLPPSTATDWAAVQRR
                     HRNKEVLRRQLLRLCEEEPQVAAALDQTVAEMNSDADQLDALLERQNYRLAFWRTAER
                     ELGYRRFFDINSLVGLRAEDERVFADTHALILDWLARGVLDGVRVDHPDGLRDPEQYF
                     HRLHTACPAAWIAAEKILEPGERLPESWPIAGTTGYDFIYRVNSLFVDSAAETALTEF
                     YGEFTGEPTDFAAVVRDKKQFVLREILASDLNRLTALLVDICERHRRHRDYTRHELHE
                     ALRELIASFPVYRTYVRAEAGVISDDDARIINRTADDAKARRPDLDAELFDFFRDILL
                     LRKSGQLETEWVMRLQQLTGPVMAKGLEDTAFYCFNRLVSLNEVGGDPGHFGLSVDEF
                     HGACVETQARWPRAMLASSTHDTKRSEDARARINLLSEIPERWIEAVKRWAELNDGYR
                     RNGFPDRSTEYLLYQTLIGAWPIETDRILAYMEKASREAKAHTSWTDPNPIYDDALRA
                     FVESVINNSEFTESLETFVTPLIEPGRINSLAQTLIKLTAPGIADFYQGSELWDLSLV
                     DPDNRRPVNYKLRRRLLAEIKCMSAEQVWSRADEGLPKMWLIRQTLKVRHERQLFQPQ
                     DSYRALTPSGAKREHTIAFARDERAFTVAPRLPLKLAGDWGDTAIEIPNGRWRNLFTG
                     EVSDGGVVSVAKLLKRFPVALLLPEEEIR"
     gene            2666..>3610
                     /locus_tag="VF089_19250"
     CDS             2666..>3610
                     /locus_tag="VF089_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019558642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6176152.1"
                     /translation="MTPFRVWAPQAQRVEIDLAGDRHLMTRADYGWWHAEVALADRGD
                     YSFVLDGGEPLPDPRSPWQPRGIHGASRMVDHKAFSWTDQRWQAGPLSAAIIYELHVG
                     TFTPPGTFAGVIEKLDYLAELGITHIELMPVAEFSGTRGWGYDGADLYAPHHAYGGPG
                     ELKRLVDACHGRGFAVILDVVYNHLGPAGNYLAQFGPYFTDRYATPWGQAINFDGPHS
                     DEVRRFVCDNALMWLRDYHFDGLRLDAVHALIDTSAIHLLEQLACEVSELEAQTGRHL
                     VLIAESDLNDPRIVRSQEIGGYGIQAQWSDDFHHALHTV"
BASE COUNT          738 a         1018 c         1055 g          799 t
ORIGIN      
        1 ggtgctgcga cgtgtcacct gaactccggg ctacgtaccg catccagtta catcgcggct
       61 tcgccttcgc cgatgccgct gcaatcaccg attacctggc tgaacttggc atcagccatc
      121 tttattgctc accctatttg caagcagctc tgggcagcac ccatggttac gacgtggtca
      181 acccgcagca ggttaaccag gaactcggcg ggatcaatgg tcatgctcgc ttatgcgccg
      241 cgctggagga aaatggcctc aagcaggtgc tcgatatcgt gcctaaccac atggccattg
      301 gcggcagaga aaacccctgg tggtgggatg ttctcgagaa cggaccgtcg agtcgctatg
      361 cgggattttt cgatgtcgac tgggatcccc ccgaagccaa actgcgcaac acggttcttc
      421 tgccggttct gggcgatcat tacggccgag tgcttgaaaa aggtgaagtc gcgctagaac
      481 gagaaggcgg gcagtttatt ttccgttatc acgatcatat ctttccggtt tcgccacgat
      541 ctctggatga gctaattgcc aatgccgccg gacggtgcgc ttcggacgac ttggcattca
      601 tcgctgaggc attgcgtcaa ctgccaccct caaccgcgac cgattgggct gccgtacaga
      661 gacgccaccg caacaaagag gttctgcgcc gccagctgtt gcgcttgtgt gaagaggagc
      721 cgcaagtcgc tgcggctctc gatcaaacag tcgctgagat gaattcggat gccgatcagt
      781 tggatgcgct cttggagcgc cagaactatc gcctggcatt ttggcgtacg gcggaacgcg
      841 aactgggtta ccgccggttt ttcgatatca acagtttggt cggattgcgg gcggaagacg
      901 agcgggtgtt tgccgatacc cacgcattga ttctcgattg gctggccagg ggcgtgctcg
      961 atggtgtgcg ggtagatcat ccggatgggc tgcgcgatcc tgaacagtat ttccatcgcc
     1021 tgcataccgc ctgcccggcg gcgtggatcg cagccgagaa gattctcgag cctggcgaaa
     1081 ggcttcctga atcatggccc atagccggaa caacgggcta tgacttcatt taccgggtga
     1141 acagtctctt cgtcgactct gctgcagaga ccgcgctgac cgaattttac ggcgagttta
     1201 ccggcgagcc taccgatttt gccgcggtgg tgcgtgacaa aaagcaattc gtcttgcgcg
     1261 agattctggc cagcgacctc aaccgtctaa ccgctttgtt ggtcgatatc tgcgagcgcc
     1321 atcgacgcca tcgcgactac acgcggcacg agctgcacga ggcgctgcgc gaactgatcg
     1381 cgtcctttcc ggtttaccgc acttacgtcc gcgctgaagc tggagtcata agcgatgacg
     1441 atgcccggat catcaaccgc accgccgatg atgccaaggc acggcggccg gacctggacg
     1501 ctgagttgtt cgattttttt cgcgacattc ttctcctgcg taaaagcggc cagctcgaga
     1561 ccgagtgggt catgcgtttg cagcaactga ccgggccggt aatggccaaa ggtctggaag
     1621 acaccgcttt ttattgtttt aaccgcctgg tttcgctgaa cgaagtgggc ggtgatccgg
     1681 gacacttcgg cctatctgta gatgaatttc acggcgcctg cgtcgagaca caggcacgct
     1741 ggccgcgcgc catgctcgct tcctcgacac atgacaccaa gcgcagcgag gacgcgcgcg
     1801 cgcgcatcaa cttgctttcg gaaattccag agcgttggat tgaggccgtg aagagatggg
     1861 ctgagctcaa tgacggctat cgccgcaatg gttttcccga tcgaagtacc gaatacctgc
     1921 tttatcaaac cctgataggc gcttggccca tcgaaaccga tcgcattctt gcgtatatgg
     1981 aaaaagcctc gcgcgaggcc aaggctcata cctcctggac cgatcctaat ccgatctacg
     2041 acgatgcttt gcgtgctttc gtcgaaagtg tgatcaacaa ttccgagttc accgaaagcc
     2101 tggaaacgtt cgtaacaccg ctgattgagc cgggccggat caattctctg gcgcagacat
     2161 tgattaaact caccgcgccc ggcattgccg atttttacca aggcagtgag ctctgggatt
     2221 taagtctggt cgatcccgat aaccgccgcc cagtaaatta taaattgagg cgccggttgc
     2281 ttgccgagat aaagtgcatg tcagccgagc aggtatggtc tcgggcagac gagggtctgc
     2341 ctaaaatgtg gttaattcgg caaacactca aagtgcggca cgagcggcaa ctatttcagc
     2401 cgcaggacag ttaccgcgca ttaacgccat ccggagcaaa gcgcgaacat accatcgcgt
     2461 tcgcgcgcga cgagcgcgca ttcacggtgg cgccgcgttt acctttgaag ctcgccggag
     2521 actggggcga taccgccatc gaaatcccaa atggccgttg gcgcaatctc tttaccggcg
     2581 aggtatcaga tggcggcgtg gtttcagtgg ctaagctgct taagcggttt ccggtagcgc
     2641 ttctattacc agaggaagaa attcgatgac tccgttccga gtatgggcgc ctcaggcgca
     2701 acgggttgaa attgatcttg ccggtgaccg gcatcttatg actcgcgcag attacgggtg
     2761 gtggcacgcg gaggtagctt tagctgatcg cggtgactac agtttcgtgc tcgatggtgg
     2821 cgagccgtta ccggatccgc gctcaccgtg gcagccacgc ggtatccatg gcgcatcccg
     2881 catggtcgat cacaaagcat tttcatggac cgatcagcgc tggcaggccg ggccgctctc
     2941 tgctgcgatc atctacgagc tccatgtcgg tactttcacg ccgccaggaa ctttcgccgg
     3001 ggtgatcgag aagctcgact atctagccga gctcggtatc acgcatatcg aattgatgcc
     3061 ggtggctgag ttttccggca cccgaggctg gggttatgac ggcgccgacc tgtacgcgcc
     3121 gcatcatgct tacggcgggc cgggtgagct gaagcggctg gtggatgcct gccatgggcg
     3181 aggctttgcg gtgattctcg atgtcgttta caaccacttg ggacccgcgg gaaattatct
     3241 agcgcagttt ggtccgtatt tcaccgaccg gtatgccacg ccctggggcc aggcgataaa
     3301 cttcgacggg ccgcacagcg atgaagttcg tcgtttcgta tgcgacaacg cgttgatgtg
     3361 gctgcgcgat tatcatttcg acggtctacg cctcgatgcg gtgcacgcgc tgatcgacac
     3421 ctcggctatc cacctactcg aacagctcgc ctgtgaggtc agcgagctgg aagcgcaaac
     3481 cggccgccat ctggtactga tcgccgagag cgatctcaac gatccaagaa ttgtccgctc
     3541 tcaagagatc ggcggttacg gcatccaggc gcaatggagc gatgattttc atcacgcgtt
     3601 gcatactgtg
//
LOCUS       DASUTG010000828         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1758687, whole genome shotgun sequence.
ACCESSION   DASUTG010000828 DASUTG010000000
VERSION     DASUTG010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758687"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF089_19255"
     CDS             complement(<1..692)
                     /locus_tag="VF089_19255"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEX6176153.1"
                     /translation="MAKDLPSFIAQEGAKREGAVIREKNFVDANNYETVAYLKHLDLR
                     NEPKMVLFENVPALNGQRSEFPMFYNPWVTRQYVADSLDMGDIKSPMDVSLEVAKLEK
                     QQGNVEIISSAKAPVKEVVLTGDKADLRILPMPMHQKGDAGPYHTMTCAMKGLDAEFY
                     DITFTKNKYHDPKHMSFSAHKHHHLEAMACEYEEKNLRAPVIVILGHHPAFYLSSCAM
                     TAMGNNDYLTASA"
     gene            complement(752..2284)
                     /locus_tag="VF089_19260"
     CDS             complement(752..2284)
                     /locus_tag="VF089_19260"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX6176154.1"
                     /translation="MSNQTIRVRNPATMEFVGELPIDSSANVAAATARARQAQAEWQV
                     RSFEDRAKALRRFRDLLIDQQDRLADIVTSETGKPRADVFGNELFYVCDAIGFWTKRA
                     EKWLKQRKIRPHLFKVKKVLSTYQPLGLVGIISPWNFPLVLTAGDAVPALMAGNAVII
                     KPSELTPFTALFVAELAQKTGFPDNLLQVVIGGAETGEALVDNVDMISFTGSVDVGKH
                     VMRRAADRLIPVSLELGGKDPMVVLKDADLERAANACVWGALMNCGQVCTSIERVYVE
                     EPIYQTFVGKVVDRVRQLRQGPSGDEVELGSMSSEEQLKKVIAQVNDALSKGATVLTG
                     GQANPSFPGLFYEPTVLVNVTHDMSIIQDETFGPVIPIIKVNNADEALRLANDSRYGL
                     DAIVFSRDRIKARQMAQSLYTGTVCINDGLVNYIIPEAPMGGVKDSGFSRRHGPEGIR
                     KYCYHKTIVIDRFGLKKEMPWFPSSRKKTAQMRRMISLLWRSGWRNKFSAPGHSASKS
                     NP"
     gene            3017..>3490
                     /locus_tag="VF089_19265"
     CDS             3017..>3490
                     /locus_tag="VF089_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008607607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6176155.1"
                     /translation="MDVTQLLAFGVEQGASDCHLSAGEPPMLRVHGDLKKLDYPPLSQ
                     EEVHNLVFDVMTDSQRKKFEETRECDFSFELREIARFRVNVFLQRKGEAAVFRTIPTK
                     VFTLEELGMPPILKQLCEKEKGLILVTGPTGSGKSTTLAAMIDYLNNGFEGHILTV"
BASE COUNT          793 a          895 c          906 g          896 t
ORIGIN      
        1 gctgacgcgg tgaggtaatc attatttccc attgccgtca tggcacagga ggagagatag
       61 aatgcggggt gatgtccgag tataacgatt actggtgcgc gaagattttt ctcctcatac
      121 tcgcaggcca tggcttccag atgatggtgc ttatgggcgg agaagctcat gtgcttcgga
      181 tcgtgatatt tattctttgt gaacgtgatg tcgtaaaact cagcgtccaa tcccttcatt
      241 gcgcaagtca tggtgtgata ggggccagca tcgccttttt gatgcatagg catcggaaga
      301 atgcggagat cggctttgtc gcctgtcagg acaacttctt tcaccggggc tttagccgag
      361 ctgatgattt caacattgcc ttgttgcttt tcgagtttgg ccacttcgag actgacatcc
      421 atgggcgatt tgatgtcacc catgtccagc gagtcggcaa cgtattggcg cgtaacccac
      481 gggttataga acatcgggaa ctcagatctt tgcccattga gcgctggcac gttttcgaac
      541 aaaaccatct tcggttcgtt gcgcaagtca agatgcttta gataggcgac agtttcgtag
      601 ttgttggcgt caacaaagtt tttctcgcga atgacagccc cttctcgttt ggccccctct
      661 tgcgcgataa agctgggtaa atccttagcc atgcatttct cctgtcctac gttgaaaatc
      721 ttctattact tagtcgaatc cccggggtag ttcaagggtt agatttactg gcggaatgac
      781 ccggagcact gaatttgttt ctccagcccg accgccagag caagctgatc atgcgacgca
      841 tctgcgcggt ttttttgcgg ctcgacggaa accagggcat ctcttttttt aaaccgaagc
      901 gatcgataac gattgttttg tgataacagt atttgcggat accttcggga ccatggcgcc
      961 ggctaaagcc actgtccttg accccgccca tgggagcctc ggggataata taattgacca
     1021 acccgtcgtt gatgcagacc gtccctgtgt aaagactttg tgccatttgc ctggccttaa
     1081 ttctgtcgcg actaaataca atcgcgtcca gcccgtagcg ggagtcattg gccagcctga
     1141 gcgcctcatc ggcattgtta acttttatga ttgggatcac ggggccgaat gtttcatctt
     1201 ggatgatgct catgtcatgg gtcacattta ccagcacagt cggctcgtaa aacaatccgg
     1261 gaaaactcgg attagcttgc ccgccggtga ggacggtggc gcctttgctg agcgcatcat
     1321 tgacttgcgc aatgactttc ttcaattgct cctctgaact catgctgcca agttcgactt
     1381 catcaccgct gggaccttga cgcagctgcc tgacgcggtc gacgactttc cctacaaacg
     1441 tttgataaat cggttcctcg acgtagaccc gttcgataga agtgcagact tggccacaat
     1501 tcatcaaggc gccccaaacg caggcattag cggcacgttc aagatcggcg tctttaagga
     1561 cgaccatagg atctttaccg ccgagctcca atgagacagg gatcaaacgg tcagcggccc
     1621 gacgcatgac atgtttgccg acatccacgc tgccggtgaa ggagatcatg tcgacattgt
     1681 cgactaatgc ttcacctgtt tctgcgccgc caatgaccac ttggaggagg ttgtctggaa
     1741 aacccgtttt ctgcgccaac tccgccacga acaaagcagt gaacggcgtt aactcggatg
     1801 gcttgataat caccgcattg ccggccatca gcgccggcac ggcatcgccg gcggtcaaga
     1861 ctagcggaaa attccatggg ctgatgattc ccaccaatcc taacggttga taggttgaga
     1921 gaaccttttt caccttgaac agatgcggcc gaatcttacg ttgttttagc cacttctccg
     1981 ccctcttggt ccagaagcca atagcatcgc aaacgtaaaa gagctcgttg ccgaacacgt
     2041 cagcgcgcgg tttgccggtt tctgaggtaa caatatcggc aagtcgatct tgttggtcga
     2101 tgagtaagtc gcgaaagcgc cgcagtgctt tggccctatc ttcgaagctt ctgacctgcc
     2161 attcagcctg ggcctggcgc gcgcgggcag tggccgccgc gacgttagcc gacgagtcta
     2221 tcggcagttc gccgacgaac tccatagtcg ccggattacg aactcgaatt gtttggttac
     2281 tcacgaccta tctccaacag cttgtcagca gaatatccgc ttttctgaaa ccataacaca
     2341 agtgtattgt aaatgggggc tccttagtcg cacagataaa gtgtcttcac acgttgttgg
     2401 tagcgcagtt atcgatcatc tgcccggtgc tacccgctct tgttctggag cgatatagta
     2461 gaccgggttt gcacctcatc aggtcgagaa atcttctgat cggagaggcg ctacttttgc
     2521 cttgagttgc actgatgaag ctcacggcgg aagccgatca ttccacgctt ggtagtgcgg
     2581 taggctgtgt attccggcga cgccgaacat tcggagtact gaggaatgtt aatgttgcgc
     2641 cagccggatt gtctaggctg gactacctct ttcggtggcc gccgattaaa ggtgagcagg
     2701 gtctcgggag gcaagtgcaa ctttggaccc gtttatggcc aattgtacac cgatggcttt
     2761 accgataagc gtgccgccac cagttcccag ggtaactctg tcgttcaaca taggcgagag
     2821 atctccctga cgtcactgat aattacctat catcgcaaga aagcgcaaat aaggattatg
     2881 cgcggccagc gctgaacctt agctggtatt gttattgctc agattcccta cagcagtagt
     2941 gatttcactc ggacctcaaa attgcccctt cttcaaatta gcgcgtgttt tcaagccgag
     3001 ctaggagtta tcgattatgg atgttactca acttttggcc tttggcgttg aacagggagc
     3061 gtcagattgc catctgagcg ccggtgagcc gccgatgctt cgcgtgcacg gagacttaaa
     3121 aaaactggat tacccgcctt tgtcacaaga ggaagttcac aatcttgtct tcgacgtcat
     3181 gactgactcg cagcggaaaa aatttgagga gactcgagag tgcgactttt cctttgaact
     3241 gcgtgaaatc gcgcggttcc gcgtgaatgt cttcttacag agaaaaggag aagcagcggt
     3301 ttttcggacc ataccgacga aagttttcac tctggaagaa ttgggaatgc ctcccatcct
     3361 aaagcaactc tgtgaaaagg agaaagggct aatcctggtc acgggcccga ccggctccgg
     3421 gaaatccacg acactggccg cgatgattga ctacctgaat aacggttttg agggtcatat
     3481 tcttactgtc
//
LOCUS       DASUTG010000829         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1909236, whole genome shotgun sequence.
ACCESSION   DASUTG010000829 DASUTG010000000
VERSION     DASUTG010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909236"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF089_19270"
     CDS             <1..588
                     /locus_tag="VF089_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176156.1"
                     /translation="QPPSCGGSGTTSSDYLVAKILELTVGANINSVLGYPGGNEADLA
                     VEKGEIACRAHTLAAHFGREPFNSWHKKGFDRHLLQTGRKRDPRAPEAPTIYEILEEF
                     KVPSGNRRIVQAMLSGGEFGRPFLVTRGTPPNRVKILRESFVRTLKDPELLDDAKKAK
                     MEVDYTSGEELEALIKEVLDQPPEIVQQVRKFMAK"
     gene            635..1999
                     /locus_tag="VF089_19275"
     CDS             635..1999
                     /locus_tag="VF089_19275"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6176157.1"
                     /translation="MHETAKIARFVVETEFKNIPSEAVKMAKTAVLDCLGVALAGSKE
                     SSARICAEIARQEHAKEESSVIGQGFKSSAMHAAFANGTAAHALDFDHSFTLMGQPTA
                     PLIPAIISLGESLGASGRRCLEAYVGGFETITKLVMSLRQRSEDGWHPPSSFGSFGAG
                     VACAKLQGLDQSQVEMTLGTAASMASGLVCNFGTMSKPLHVGLGARNGVVAAKLAQSG
                     FTANKQAIEAGLGFYQVFYPGAEPDTRPLDELGTAFELINSGIRIKPYPCGGLTHPAI
                     DGVLEFKTKHGISAEMVESIEVGVARHTFERIVFRVPENGLQGKFSMPYLLARAIIDG
                     KLFLDAFTDSAVKDKNVLRLAEKIQMHLDPDLQPTAQGSRPCKVAIRLLDGRSFSRQI
                     DYAKGSREAPMTAEELKQKFVDCAREAIDESSIERVMEDIEHLETLDDIRPLCQLLIG
                     SNKS"
BASE COUNT          466 a          545 c          621 g          431 t
ORIGIN      
        1 cagccgccca gctgcggcgg atccgggacc accagttccg attatttggt cgcgaagatt
       61 ttggagctga cggtgggcgc gaacatcaat tctgttttgg gttaccccgg cggcaatgag
      121 gcggacctgg cggttgagaa aggtgagatc gcttgccgcg ctcatacgtt ggcagctcac
      181 ttcggccgcg agcccttcaa tagttggcat aagaagggct tcgatcgcca tttgctacaa
      241 accggacgta aacgcgaccc gcgcgcgccc gaagcgccga cgatttacga gatcctcgag
      301 gaattcaaag ttcctagcgg taatcggcgc atcgtccaag ccatgttgtc cggcggggag
      361 ttcggccgcc ccttcttggt cacacggggc acgccgccga accgcgtgaa gattctgcgt
      421 gaatctttcg tcaggaccct caaggatcca gagctgttgg acgatgccaa aaaagccaag
      481 atggaagtcg attatacttc cggcgaggaa ttggaagctt tgattaaaga ggtgttggat
      541 caaccgcccg agatcgtgca gcaggtaagg aaatttatgg ccaagtaaac accggcgaag
      601 cagcaggtca acggacgaaa agatcggagg tgttatgcac gagaccgcga aaattgcccg
      661 cttcgtagtc gagactgaat tcaaaaacat tccgtcggaa gcggttaaga tggcgaaaac
      721 agcggtgctt gattgtctgg gtgtggcatt ggcgggcagc aaagaatcga gcgccaggat
      781 ctgcgccgaa atcgctcgcc aggaacacgc gaaagaagag tccagcgtca ttggacaggg
      841 atttaaatca tcggcgatgc atgcggcttt cgccaatggc acagcggcgc acgcgctgga
      901 tttcgatcac agctttacgc tgatgggcca acccacggcg ccgctcattc cggcgattat
      961 atctttggga gaatcgctcg gtgccagcgg ccgccgttgt ctcgaggctt atgtcggcgg
     1021 gttcgagacg attacgaaac tggtcatgtc gctgcggcag cgctccgagg atggctggca
     1081 tccgccgtca agcttcggtt ctttcggcgc cggcgtggcc tgcgcgaaac tgcaggggct
     1141 cgaccaatct caagtagaaa tgactctcgg cacagcggca tcgatggcca gtggactggt
     1201 gtgtaatttc ggcacgatgt ccaaaccgct gcacgtcggg ctgggtgcga gaaacggagt
     1261 ggtggccgcc aagctggcgc aatccggttt caccgccaac aagcaggcca tcgaagcggg
     1321 actcggtttc tatcaggtat tctatcccgg agcagagccg gacacgcgcc cgctcgatga
     1381 gttggggacg gccttcgaat tgatcaacag cggcatccgg atcaagcctt atccctgcgg
     1441 cggcctgacg catccagcca tcgacggcgt gctcgagttc aagaccaagc atgggatcag
     1501 cgccgagatg gtcgagtcca tcgaagtcgg tgtggcgcgt catacgtttg aaagaattgt
     1561 ttttcgggtg cctgagaacg gcttgcaggg taaattttcc atgccgtatc tgctggcgcg
     1621 cgcgatcatc gatggaaaac tgttccttga cgcatttacc gattcggcag ttaaggataa
     1681 gaatgttttg cgtttggctg aaaaaattca gatgcacctc gaccccgacc tgcaaccgac
     1741 tgctcaaggc agccgtccgt gcaaggtcgc cattcgtctg ctcgatggcc gaagtttttc
     1801 ccggcagatc gattacgcca aaggcagccg cgaagctccg atgactgccg aagaacttaa
     1861 acaaaaattt gttgattgcg cgcgtgaggc tatcgatgaa agttccatcg aacgggtcat
     1921 ggaggacatc gagcatctgg agactttgga cgatatccgg ccgctgtgtc agttgctaat
     1981 cggatcaaac aaatcttagg atcgacactg gagggagatc agcctgaccg cgatccttcg
     2041 tcctgctgcc gctcgatgag ttg
//
LOCUS       DASUTG010000830         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_277211, whole genome shotgun sequence.
ACCESSION   DASUTG010000830 DASUTG010000000
VERSION     DASUTG010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277211"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1043
                     /locus_tag="VF089_19280"
     CDS             474..1043
                     /locus_tag="VF089_19280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6176158.1"
                     /translation="MSGERKAGGAKRGDVAKEAMVHLDHLYRVAFFLAKNNSDAEDLV
                     QETFARALAANEQFEPATNMKAWLTTILRNFFFDHYHQKQRWVSAEELSEPQSDYWEG
                     LAAENPGPERTILIRELDDQITAVLRKIPEEFRTPIVLVDVSDFSYEEAAVILSCPIG
                     TVRSRLSRGRKLMRKYLTAYVVGGQDKLK"
     gene            1046..1858
                     /locus_tag="VF089_19285"
     CDS             1046..1858
                     /locus_tag="VF089_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6176159.1"
                     /translation="MNCDEAQESITALVDQELGHAERSVLEAHMGECSGCRLAFEEEL
                     DLKRKIRQAGERMHAPALLRSRILSDRRIFPEKSQSPRQWLKTLWPGSPVYQTALALT
                     VLLLLLLPTYYLSNRISQPVAMAAFETYDSFTRGELPVERTQNSDEIVGRLTRAVDGR
                     FHPMGYDLSAMDLTPVAGMVREAQGRKILVAIYQGEGGSLLCYTFLGSEADAPPNAAR
                     FFDPNKKMNFYAFSRAGINAVLHREGDVICILASEMPMDDLLALARKKSRPS"
     gene            1884..1982
                     /locus_tag="VF089_19290"
                     /pseudo
     CDS             1884..1982
                     /locus_tag="VF089_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC-related (seleno)protein"
     gene            2134..2628
                     /locus_tag="VF089_19295"
     CDS             2134..2628
                     /locus_tag="VF089_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX6176160.1"
                     /translation="MIFRTMRHLGPAALLAATLFIANQARPTIAADYPYPSTLTAPTP
                     HQFDALLKRLERAIADNGMGLVAQASASRGAAARGVTIPGNAVLEVFRNDYAVRMLEA
                     SVPAGIEAPLRIYVIENADKTATIIYRRPTAVFAPYGNTKLDAMARELDPIFEKIVRA
                     AASN"
     gene            complement(2874..3626)
                     /locus_tag="VF089_19300"
     CDS             complement(2874..3626)
                     /locus_tag="VF089_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263013134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3432..3434),aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6176161.1"
                     /translation="MMKRNHYNYESFGQCDLAAPDFHSVANVGSRAPQFTLRSLDGEK
                     VSLADFIGKGHVVLEFGSITUPPFVGEVPPLTELYQKYRARGFEFFIVYTREAHPGEN
                     FPHHVSLEQKVSHAKQLKELDEVKINVLVDDFEGSTHNAYGALPNMIYLIDRDGVVVY
                     KSDWTDAHELDGMCESLVRLDDMKARQVPIIRTGVSQRLHWIPMDPRHRERIYRRSGE
                     KAIQDYKNARGSLPYATDVEKADAQAATGKIA"
     gene            complement(3685..3807)
                     /locus_tag="VF089_19305"
     CDS             complement(3685..3807)
                     /locus_tag="VF089_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176162.1"
                     /translation="MLQPYSDAAQELEQAAHTGYRGGKWQTMAIGQTHIMVMYK"
     gene            4010..4195
                     /locus_tag="VF089_19310"
     CDS             4010..4195
                     /locus_tag="VF089_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176163.1"
                     /translation="MGLKEDISQPQSYYWERLPAENPAERRVRSGNWTNQITDGLRRN
                     SEEFRMRVVLVDGAIAR"
BASE COUNT         1119 a         1253 c         1199 g         1070 t
ORIGIN      
        1 atgatgagtt tgccgcagtc ccgctcctga ttgtctttga cgaagagacc gccaccggag
       61 tgatttttac aagcttgatg gaaagaccct ttcattcaaa aaagctcagc ccaaaagcga
      121 aaaggggctg tttttggttg accacgaaag tggttcgatc tggcaagggc tgaccgggca
      181 agcggtacag ggctcgctca aaggaagcag gctcgaggcg ttaccgatca ccccatcctt
      241 ctggtttggt tgggtcgatc actatccgga cacgcagctt tttgaattgc ggaaataaac
      301 tgcatcaata cagagcgaag ccctcttaac gacggtggaa agaaaattcc ctcccaagtc
      361 agagcggtca tggcgattac ccgatatcat tgggaactta ggatgcacga aagcgttatc
      421 gttatatggt tttagacagt ggctaatcta aatcttttcg ttagaatgcc cccgtgtcgg
      481 gagagagaaa agcagggggc gctaagcggg gcgatgttgc aaaagaagcc atggtccacc
      541 tcgatcattt atatcgggtg gctttttttc ttgcaaaaaa caattcggac gccgaggatc
      601 tagtgcaaga aacctttgcc cgcgcccttg ccgccaacga acagttcgag ccggcaacta
      661 atatgaaagc atggctgacg acgatcctgc gcaatttttt tttcgatcat tatcatcaaa
      721 agcaacgatg ggtctcagca gaagagttat cggagccgca atcagattat tgggaagggc
      781 tagcggcgga gaatcccgga ccggagcgca cgatcttgat ccgggaactc gacgatcaaa
      841 tcaccgctgt gctgagaaaa ataccggaag aatttcgcac acccatcgtt ttggtggatg
      901 ttagcgattt ctcctatgaa gaagccgcgg tcatattatc atgcccgatc ggcacagttc
      961 gctcgcggct ctctcgggga agaaaactca tgcgaaaata cctgacggcg tatgtcgtcg
     1021 gcggacaaga caaacttaag tgaagatgaa ctgcgacgaa gcacaggaat ctatcaccgc
     1081 gctggtggat caggaactcg gtcacgcaga acgatccgtg ctggaagcgc acatgggcga
     1141 atgctccggc tgcaggctgg cgtttgaaga ggagctggat ctgaaacgaa agatccgcca
     1201 agccggcgag cgcatgcatg cgcccgctct cttgcgcagt aggattcttt cggaccggcg
     1261 aatttttcct gagaaaagcc aatcgccgcg gcaatggtta aaaaccctct ggcccggttc
     1321 tcctgtttac cagaccgccc tggcgctaac cgttctactg ctgctccttc tgccgaccta
     1381 ctatctatcg aatcggatct ctcagccggt ggcgatggcc gccttcgaaa cttacgactc
     1441 attcacgcgc ggcgaactgc cggtggagcg gacgcaaaac agcgatgaaa ttgtcgggcg
     1501 gctgactcgc gcggtggacg gacgctttca ccccatggga tacgatcttt cagcgatgga
     1561 tctgacaccg gtggcgggta tggtgcggga agctcagggt cgcaagatcc tggtcgcaat
     1621 ctatcagggc gaaggaggtt ctctgttatg ctacaccttc ctcggatcag aagcagatgc
     1681 cccgccaaac gcagcgagat tttttgatcc gaataaaaag atgaattttt atgccttttc
     1741 acgggccggt atcaatgccg ttctccaccg cgaaggcgat gtgatctgca tccttgcttc
     1801 cgagatgccc atggatgatc ttctggctct ggcgagaaaa aaatccaggc cgagttaagc
     1861 tcaatcgcgg cggcaccaac cgtaaaatcg cggctgttgt acgcaatgca gagaacggct
     1921 gctatgtgat gcaatgtctg cttaacccag tacccgtcga gcgcagcgtt cagttgaatg
     1981 gcatggactt caattccaca gatgccaatc aacaaagagt gagaattttc cgtccggctc
     2041 ggtttaggca cttatcatgc gagtcctacg atgcagcacc tttgattaat ttggcgggac
     2101 tttcaggtca ccaactgacg tgagaggaaa tccatgatct tcagaactat gagacatctc
     2161 ggcccagcag cattactggc tgcaacgcta ttcatcgcga atcaggccag acccacgatc
     2221 gccgccgatt atccgtaccc cagcacttta acagcgccca cgccgcacca attcgatgca
     2281 cttcttaaac gcctggaaag ggccatagcc gacaacggca tggggttggt ggcccaagcc
     2341 agcgcgagcc gaggcgccgc ggcacgcggc gtcaccatcc ccggtaacgc agtgctcgaa
     2401 gttttccgca atgactatgc ggtgcgcatg ctcgaggcga gcgtaccggc cggcatcgag
     2461 gcgccgctgc gaatttatgt gatcgaaaat gccgacaaga ctgccaccat tatttaccgg
     2521 cgccccactg cggtgttcgc accttatggc aacaccaaac tcgatgcgat ggcccgtgag
     2581 ctcgatccca tctttgaaaa gattgtccgt gctgccgcaa gcaactgacg tgaccacaaa
     2641 atcgtgccag acagaactcg ggcaatgaac cgtctccgga agtacgtact tctatctgac
     2701 ttgccaccgg agttagtccg cgatcggtgc aatcaatctc tataaaagcc gaaccgtccc
     2761 cccttacaaa cggaccgttc agagatcaat gaccgcaagg cttttgctgt gtcgggtttt
     2821 taatatccga cgcaaaaatg gcgcagataa ccgtaagctt gggacgatta ctcttaagct
     2881 attttccctg tcgcagcctg cgcatctgcc ttttcaacgt cggtagcata tggaagactt
     2941 ccccgggcat ttttgtagtc ttggatcgcc ttttccccgg aacgccggta tatccgttcc
     3001 ctgtgcctcg gatccatggg aatccaatgg agccgctgtg acacgcctgt acgaatgatc
     3061 ggcacctgac gagctttcat atcgtccaac ctgacgagac tttcgcacat accatcaagc
     3121 tcgtgggcgt ctgtccaatc ggacttgtaa acgacgacac cgtcacgatc gatcagataa
     3181 atcatatttg gcaaagcgcc ataggcgttg tgagtgctac cttcgaaatc gtcaaccaga
     3241 acatttattt taacttcgtc gagctccttt aactgcttcg cgtgggatac cttctgctcc
     3301 aatgacacat gatgcgggaa gttttctccg ggatgagcct ctcgcgtata gacgataaaa
     3361 aactcaaatc cccgcgcgcg gtatttttga taaagctcgg tgagtggagg aacctccccc
     3421 acgaaaggcg gtcaagtgat gctgccgaac tccaacacga cgtgcccttt tccaataaag
     3481 tcagccagtg agactttctc tccatccaag ctgcggagcg tgaattgcgg agctcgactt
     3541 ccgacgttgg caaccgaatg aaaatcaggc gcggctaaat cgcattgccc gaaagactcg
     3601 taattgtagt gattccgctt cataatttcc ccttatttga taagtagggt ttcttgtctc
     3661 tccccttatc tcttttatgc ataatcattt atacatcacc atgatgtgag tttgaccgat
     3721 cgccatagtc tgccatttcc cacctcgata accggtatgg gccgcttgtt cgagttcctg
     3781 ggcggcgtcg ctgtagggct gcagcaccac catctccaaa tcgggaacgg cgacgttgcc
     3841 actggctccg ttgacttttg aagtctatcc ctatccttgc tgattctcct ttatcggtaa
     3901 ctagggtaga gattatcgcg ctgcgcccgc aagcgccaac agcaaagtcg ctgcgtcatg
     3961 acgaagatcc tgcacaattt ttcttcgatc attaccgtca aagaagacaa tgggtctcaa
     4021 agaagatata tcacagccac aatcctacta ttgggaacgc ctgccggctg aaaatcccgc
     4081 cgaacgccgt gttcgctcag ggaactggac aaatcaaatc actgatggcc tgagaagaaa
     4141 ttccgaagag ttccgcatgc gcgtcgtttt ggtcgatggg gcaattgcaa gatgaatttt
     4201 tatgcctttt cacggggggg tatcgatgcc gttctccacc gggagggcga tgtgatctgc
     4261 atctggcgtc cgagacggct agggatgagc ttctgcctct ggtaaaatcc aaggcttggc
     4321 cgagttgata tcactctcgc cgattccgct taccgtccgg accgtgtcaa agaggacacc
     4381 gcaaaaaccg ggcggaactt gaatctctcg gcggcgctat gtataggagg tcggcgaccg
     4441 gcgcagcacg ctacgcgcga agaaaacgag tgcatcttta actaataact aggcgtcacc
     4501 gtgattctgc tcctacccga ctccctgtgg ctgagacatc gtggatatgg catcgccgcc
     4561 ggcgctgaca gggagcgaag acatcgacat catctgccat gaggttgtgg cccgctctcg
     4621 aacactgttc gagagcctac t
//
LOCUS       DASUTG010000831         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_277507, whole genome shotgun sequence.
ACCESSION   DASUTG010000831 DASUTG010000000
VERSION     DASUTG010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277507"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /gene="aspS"
                     /locus_tag="VF089_19315"
     CDS             complement(<1..816)
                     /gene="aspS"
                     /locus_tag="VF089_19315"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6176164.1"
                     /translation="MAIERVFDDQLGDWKRSCYCGEPRAEAVGRELVLTGWVHTRRDH
                     GGLIFVDLRDRSGITQIVFNPEVDLTAHERAKQLRSEDVIAVRGVLSRRPAETLNPDL
                     ATGEVELAGKELRLLNASQPPPFLIDDETDANENTRLKYRYLDLRRPRSLAHLLLRYR
                     MTKLIRDYLDAHGFIDIETPVLTKSTPEGARDYLVPSRIYKGKFYALPQSPQLFKQIL
                     MVGGLDRYFQIVKCFRDEDLRADRQPEFTQLDMEMSFTQAADVIGIVEGMIVLL"
     gene            complement(817..2079)
                     /gene="hisS"
                     /locus_tag="VF089_19320"
     CDS             complement(817..2079)
                     /gene="hisS"
                     /locus_tag="VF089_19320"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6176165.1"
                     /translation="MKVTAIKGFSDILPGEVEAWQRVEREARKIFAAYNFAEIRIPIV
                     EKTELFSRSLGETTDIVEKEMYTFADQDAKGSLLTLRPEGTAGVVRAYVQAELFKTEP
                     VQKLYYLGPMFRRERPQKGRMRQFHQIGAEVFGRGDPFVDAEVLLLLNDFFSAAGIES
                     ALQLNSLGCSDCRPKYRQLLLRFLTEREEALCANCRRRMERNPLRVLDCKESGCIEQT
                     KNAPSILQSLCDPCRQHFDTVQRLLRQSALGYDLNPRMVRGLDYYCRTTFEWTSDQLG
                     SQSAIAAGGRYDGLVQELGGPAIAGIGFAIGVERLTMLLRSRGGPAPAGPVVYTVWVG
                     ENAREWAFPVVHRLRQRGLSIEIEGEVRSLKSQMRRADKLKASSVLIIGDNELDRGVA
                     LLRDMASRQQSEISLNDVEEVLLSRKAN"
BASE COUNT          569 a          687 c          595 g          533 t
ORIGIN      
        1 aagtaaaacg atcattccct ccacgatgcc aatcacgtca gcggcctggg taaaagacat
       61 ttccatgtca agctgagtaa actcaggctg ccggtcagcc cggagatctt catcgcgaaa
      121 acatttgact atttggaagt agcggtcgag gccgccgacc atgaggatct gcttgaatag
      181 ctgcggcgac tgcggcaacg cataaaactt acctttgtaa atacgacttg gaaccagata
      241 gtcccgcgcc ccttccggag tgctcttggt cagcactgga gtctcgatgt cgatgaaacc
      301 gtgcgcatcc aaatagtcac gtattaactt ggtcatccgg tagcgtagca gcaggtgagc
      361 taaactgcgg ggccgacgta agtcaagata acggtacttc aaccgggtat tctcgttggc
      421 gtcggtttcg tcatcgatca aaaaaggcgg cggctgtgag gcgtttagca gtcgcaattc
      481 tttgccggcc agttcgactt caccggtggc cagatcaggg ttgagagtct ccgccgggcg
      541 ccttgagagc acaccgcgca cggcgatcac gtcttcggag cgcagctgtt tggctctttc
      601 atgggcggtt agatcaactt ccggattaaa aacgatctga gtaatgccgc ttcgatcccg
      661 taaatcaacg aagattagcc cgccatgatc acgtcgtgta tgcacccaac cggtcaggac
      721 caattcgcga ccaactgctt cggcgcgtgg ctcaccgcaa tagcagctgc gtttccaatc
      781 accgagctga tcgtcaaaaa ctctctcgat tgccatttag ttagccttcc tcgacagcaa
      841 cacttcctca acatcgttca aacttatctc actttgttgc ctgctcgcca tgtcccggag
      901 caaagctact cctctatcaa gctcattgtc gccgatgatg agaaccgatg atgccttgag
      961 cttatcggcg cgtctcattt gactcttgag gcttctaacc tcgccctcaa tttcgatgga
     1021 taatcctctc tgccgtagcc ggtgaaccac aggaaaagcc cactcgcgag cattctctcc
     1081 gacccaaact gtatagacca ccgggcccgc gggagccgga cctcctctgg atcgcagtaa
     1141 catggtcaag cgctcgacgc cgatcgcaaa gccaatcccc gcaatcgccg gcccgccgag
     1201 ttcctggacc aagccgtcat agcggccgcc ggctgcaatg gcgctctgcg accctaattg
     1261 atcgctggtc cactcgaaag tagtgcggca ataataatcc agcccccgca ccatgcgcgg
     1321 gttcaaatca taacctagcg cagactgccg cagcagccgt tgaaccgtgt caaaatgttg
     1381 acggcaagga tcgcacaagg attgtagaat tgagggagca ttcttggtct gctcaataca
     1441 gccggattct ttgcagtcga gcacccgaag cggattgcgc tccatccgac ggcggcagtt
     1501 agcacagagc gcttcctcac gctccgttag aaagcgcaac agcagctgac ggtatttcgg
     1561 acggcaatct gagcagccga gggaattgag ctgcaacgcg ctttcaatgc cggcggcaga
     1621 aaagaaatcg tttaaaagca ataggacttc ggcatcgaca aacgggtcgc cacggccgaa
     1681 cacttcggcg ccgatttgat ggaattgacg catccgacct ttttgcggtc gctcgcggcg
     1741 aaacatcggt ccgagataat agagtttctg caccggctcc gtcttgaaca actctgcttg
     1801 cacataggct cgtaccaccc ctgccgtgcc ctccgggcgt aatgtcaaaa gcgaaccctt
     1861 tgcgtcctga tcggcgaacg tatacatctc cttttccacg atatcagtgg tttcgccgag
     1921 tgaccgacta aacagttcgg ttttttccac gatcggaatt cggatttcgg caaagttata
     1981 cgccgcgaaa atcttgcgcg cctctcgttc aacccgctgc caggcctcga cttccccggg
     2041 taaaatatcc gagaaacctt tgattgcggt gactttcatg aagctttact tggtcctttt
     2101 cagatcgtaa gcctgatcag gcttacgaaa gatcccacgg tcacggctcg caaactcggc
     2161 gagcggagct gcagacaaag cgctacgctg atgttagcaa acgtaagagc gatgtggatg
     2221 gaacccgaaa tacggttact ctagacacca taagaaatca ttcaggtcaa gcaaaccagc
     2281 tttccagatc caccggttgc aaatccgact gtttcattat cgttgcgcct tgtacctata
     2341 cacacggcta cggcgcgtac tgccgtgaag ttcggcaaat aaaa
//
LOCUS       DASUTG010000832         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1910439, whole genome shotgun sequence.
ACCESSION   DASUTG010000832 DASUTG010000000
VERSION     DASUTG010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910439"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF089_19325"
     CDS             <1..482
                     /locus_tag="VF089_19325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX6176166.1"
                     /translation="FHRYPLAYWEIIQNKAREAELDPYLVVALIRQESLFDTRARSSA
                     AALGLMQLLSTTATRVAKQLGLTPPSTETLFDPELNVTLGTRYLQNLLQRYSNNWFKA
                     IAAYNAGEAAVDRWERDIATEDIEEFVERIPYLETRQYVKLVLRNHRIYKTLYNQDP"
     gene            479..1249
                     /locus_tag="VF089_19330"
     CDS             479..1249
                     /locus_tag="VF089_19330"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6176167.1"
                     /translation="MSPALPSRLPLIGITADSSGCEQTGSKTKREGFYSLASRYCDAI
                     ENAGGLPIIIPHSRSRTQIHHLLRRIDGLLISGGGFDIDPAYYCEQPIAKLGKINPER
                     TFTELEAIGFALDRDLPMLGICGGAQAINVALGGSLYQDIPTQCPAALKHQQEERSPN
                     YGHVVEVARGTLLYKICRRRRLKVNTTHHQAICKTGEGIAINAIAPDGLIEGIESKNH
                     SFVLGLQWHPEVLAHTEVLQRKIFSSFVSACGRLRRAF"
     gene            1503..2639
                     /locus_tag="VF089_19335"
     CDS             1503..2639
                     /locus_tag="VF089_19335"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6176168.1"
                     /translation="MADQGGDVLDKDAAERALFPASGLPGGGQLEVRMEISANHPDPD
                     IRRVARKVKPYNLHSWHSEWSAVAVKNEELAAGFVAEERNVTAHEFYLRAADFYRRAL
                     VYLPDSDSRMLPAYRKLGETFEKAWSLVPAPFERVKIPYEGHLLEALFFPGRGKAGAR
                     LPVVYNYGGADGILLRGEDGGAGQYVRRGMHFIDVDGPGHGGALREKKLYAPADSERV
                     AKAVIDYLVTRPDVDPDRIGLHGSSMGGYSGPRCATAEKRIKAVAVWSGAYNLVDDIF
                     DYYPPIQDRLRWLIGAENLHEARERIKEFTLAGRANRIECPLLVGYSHDDRVMDPRGA
                     LKLYENAVASPDRSMLDGVGHGEKRFERRTYIADWFMKQLKAGQ"
     gene            complement(2658..3101)
                     /locus_tag="VF089_19340"
     CDS             complement(2658..3101)
                     /locus_tag="VF089_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6176169.1"
                     /translation="MDWKDNLIESRAQIRQLIMNTHRIAVLGIKTEAQAGQPSVYVPQ
                     YLLRAGFEIIPVPVYYPEVTEILGAPVYRRLVDVSGEVDLVDVFRRPQDIDGHIEDIL
                     SKRPKAVWFQSGIRNDQAAETFARAGIQVVQDRCLMVEHRSIAAR"
     gene            3290..>3926
                     /locus_tag="VF089_19345"
     CDS             3290..>3926
                     /locus_tag="VF089_19345"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX6176170.1"
                     /translation="MADLKVIGKAVTREDGVDKVSGRTLYTADVHLPGLLWGKILRSP
                     YPHARIARIETGKAQQVTGVKSIITGADVKGCLIGKQIRDMPALCWDRVRFIGDRVAA
                     VAAETIDAAEEALNLIEVDYQELPAVFDPLEAMQPDAPRLHENVATYDGAPKDLLALD
                     LPNGLTRLAWRKGDLERAIDQADVVLEHTFRIPARHQGYIEPHAAIVSIDQD"
BASE COUNT          911 a         1103 c         1089 g          823 t
ORIGIN      
        1 tgttccatcg ttatccgttg gcttactggg aaataatcca aaacaaagcg cgtgaggcag
       61 agcttgatcc ttatctcgtg gtggcgctga tccggcagga aagcctcttc gacacccgcg
      121 cccgatcctc ggccgcagca ctgggactca tgcagttgct gtcgactacg gcgacccgcg
      181 tggcaaaaca acttgggcta acacctccct caactgaaac actcttcgac ccggagctca
      241 atgtcaccct tggtactcgc tatttacaga atctattgca gcgctattca aataattggt
      301 ttaaagcgat cgccgcctac aatgccggcg aagctgccgt agaccgatgg gaaagagata
      361 tcgccaccga ggacattgaa gagttcgtcg agcgaattcc gtacctggaa acgcgccagt
      421 atgttaaact cgtgcttcgc aatcaccgaa tctataagac tttatacaac caggatccat
      481 gagcccggcg ctaccgagcc gactgccgct gatcggcatc accgccgatt cctcaggctg
      541 tgaacaaacc gggtccaaga caaaacgcga ggggttttat tctctagcta gccgctactg
      601 cgatgccatc gaaaacgccg gtggtttgcc aatcatcatt ccccactcgc gatcgcgaac
      661 tcagattcac catctattga ggcgcatcga tggactgctc attagcggcg gcggtttcga
      721 tattgatccg gcttattatt gcgagcagcc aatcgccaag ctcggcaaga tcaaccccga
      781 aagaacattt accgaactgg aggcgatcgg ttttgcactt gatcgagact tgccgatgct
      841 cgggatctgc ggcggcgcac aggcgatcaa tgttgccctg ggtggctccc tttaccagga
      901 tatcccgacg cagtgccccg ctgcgctaaa gcatcagcag gaggagcgca gccctaacta
      961 cggccacgta gtcgaggtgg cgcgcggcac tctcttgtac aaaatctgcc gccgccgaag
     1021 actaaaggtc aacacgaccc accatcaggc gatttgcaag acaggagaag gtatcgccat
     1081 caacgcaatc gcgccggacg ggctgatcga gggtatcgaa agcaaaaatc actcgtttgt
     1141 tctagggctg caatggcatc ctgaagtcct ggcacacact gaggttctcc agcgaaaaat
     1201 tttttcatcg ttcgtttcag cctgcggacg tctgcgtcgc gccttctaac ggcactccca
     1261 tccaaagagt tccacctttt acacagcacg gaacagcttc cagccgtttg cgcggctccc
     1321 actctgatag cagcaatcgc ctgtttcgca ctggctctgg tgaaatgtat cagcacgagc
     1381 ctcgcgccga ataaattcca tcacgtcggg gtctatcgct ccgcttgctc tgccgttgaa
     1441 attttagccg gtctggggca agacaatgcc agccggttca gtttagatta tggaggtaaa
     1501 tcatggccga tcagggcggt gatgttttag ataaagacgc tgccgaacga gcgctgtttc
     1561 cggctagcgg cctgcccggg ggcggacagc tggaagtgcg catggagatt tcagccaacc
     1621 acccggaccc agacatccgc cgggtcgctc gtaaggtcaa gccatacaat ctgcacagct
     1681 ggcactcgga gtggagcgcg gtggcggtga agaacgaaga gcttgccgcg gggttcgttg
     1741 ctgaagagcg caatgtcacg gcccacgagt tttacttgcg cgctgcggat ttttatcgtc
     1801 gagcgcttgt gtatctgccg gacagcgatt cccggatgct ccctgcctat aggaagcttg
     1861 gagaaacctt cgaaaaggca tggagccttg tgcccgcgcc tttcgaacgg gtaaagatcc
     1921 catacgaggg ccacctgctt gaggcgctct tcttcccagg ccgcggcaaa gccggagctc
     1981 gcctgccggt ggtttacaac tacggcggcg ctgacggcat tctcttgaga ggcgaagacg
     2041 gcggcgccgg tcagtacgtg cgccgcggca tgcattttat cgatgtcgat ggacccggac
     2101 acggcggcgc gctccgggag aaaaaacttt atgcgccggc ggattccgaa cgagtggcga
     2161 aagcagtcat cgattacctg gttacacgcc ctgacgttga tcccgatcgc attggcctgc
     2221 acggctcgag catgggcggc tattccggcc cgcgctgcgc cactgcggaa aagcgcatca
     2281 aagcggtcgc agtgtggagt ggcgcttata atctagtcga cgatatcttc gattattacc
     2341 cacccataca agaccgtttg cgctggctaa tcggcgccga gaatttgcat gaggcgagag
     2401 agagaataaa ggaattcact ttggcaggtc gcgccaatcg gatcgaatgc cccctgctgg
     2461 tgggctatag ccatgatgac cgggtcatgg atccgcgcgg cgcgcttaag ctctatgaga
     2521 atgcggttgc ttcgccggat cgctcaatgc tggatggagt cggccatggt gagaagcgtt
     2581 tcgaacgacg cacctacatt gccgattggt tcatgaagca gctgaaagct ggacaatgat
     2641 gaaataaaag cggcaggtca tctagcagct atcgagcgat gttcaaccat caaacagcga
     2701 tcctgcacga cctgaatgcc ggcgcgagcg aacgtctcgg ccgcttgatc gttgcgaatg
     2761 ccggattgaa accacacagc tttgggtctc ttcgacagaa tatcttctat gtggccgtca
     2821 atatcctgcg ggcggcgaaa gacgtcaacc agatcgactt ctcccgaaac gtcaaccaag
     2881 cgccgataga ccggcgcccc aaggatctct gtcacttcag ggtaatagac cggaacggga
     2941 ataatttcga agccggctcg gagcagatac tgcggcacgt aaaccgacgg ttgacccgcc
     3001 tgcgcttctg tcttgatacc gagaacggcg atgcggtggg tgttcatgat caattgtcgg
     3061 atctgcgccc ggctttcaat cagattgtct ttccagtcca tgcacgcaat atagcaactg
     3121 ccgcctcgac aagcgatgcg ggatttcttc cgagcccgtt gaaacggtta atgtgatgca
     3181 aagcagcaaa tctcatcttc aagcttaacg atagtcacta acctctcgag gaccatcgct
     3241 acggtctaat ttgcgacaac caacgcaaca tgctgatgcg gattttaaaa tggccgactt
     3301 gaaagtaatt ggcaaagccg tcactcggga agacggcgtc gataaggtta gcgggcggac
     3361 tctctataca gcggacgtgc atctacctgg gctgctctgg ggaaaaatct tgcgcagtcc
     3421 ttatccccat gcgcgcatag cgcgcatcga gacaggcaaa gcgcaacaag tcacgggagt
     3481 aaagtcaatc atcaccggcg cggacgtcaa gggttgcctg atcggcaaac agatccgcga
     3541 catgcctgcc ttgtgttggg acagagtccg ctttatcggc gaccgggttg ctgccgtcgc
     3601 cgccgaaaca atagacgcgg ccgaggaagc tttaaatctg atcgaggtag actaccagga
     3661 attgcccgct gtctttgatc ctttggaggc gatgcagccg gatgcgccgc gcctgcacga
     3721 aaatgtcgca acctacgacg gcgcgcccaa agatctgttg gctctggacc tacccaacgg
     3781 cttaacccgg ctggcgtggc gtaagggaga cctcgagcga gcgatagatc aagcagacgt
     3841 agttctggaa cataccttca ggataccggc ccggcatcaa ggctacatcg agcctcatgc
     3901 ggcgatcgtg agcattgatc aagata
//
LOCUS       DASUTG010000833         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_426081, whole genome shotgun sequence.
ACCESSION   DASUTG010000833 DASUTG010000000
VERSION     DASUTG010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426081"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2971)
                     /locus_tag="VF089_19350"
     CDS             complement(<1..2971)
                     /locus_tag="VF089_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176171.1"
                     /translation="MTNGRYIGTLKQAGFFAFFCTQFLGAFNDNFYKIVVSLVALNAT
                     TPQSDSLYVDLIAFLFILPSALFSGYAGHLADVYSKRSILVTVKCFEILIMGMGFAAF
                     LSGYVEPMLVIVFLMGLHSAFFSPAKYGILPEMLPETELSRGNGLLEMSTFMAIILGV
                     SLGGAIYSIWKDQLPLVGLIMVAIAVLGTMTSLKIYPVPASGSTKRFNLNPLGEIWDG
                     IKRLYPDRPLRLTVIGISYFWFLGALVQLAMLFFGKELLRLDELHISLLGTFLAIGIG
                     IGSLAAGKLSGDKIELGLVPLGSISMGLCLLLVSYSASSYFYTSASLVMLGFSGGLFA
                     VPLNALLQHRSGREEKGQLIATNNFFNTIGIILAAAIHWFLNSALQLSPDWIVFLIGI
                     LTLIATAIVLVVLPDYFVRLVAWLLTHTLYRIRVVGEENAPVGTPALLVSNHVSFADA
                     LLIGACLPGFVRFILHRDYYENKWLNWFFRLMKSIPIRATHRRDVVNSLKRARQELEA
                     GHVVCIFAEGAITRTGHILPFKRGFEKVVEGTSVPIIPVHLDQVWGSVFSFHGGRFFW
                     KWPKLFPYPVTVSFGKPLQANSTANQVRQAVLELASEARRYRRKPTDLLPAKFVQMAK
                     RRWFSFCMADTTGAELTYGKTLIGSMLLCRWVSEHCRKDRMVGVMLPASVGGALANLA
                     IMLAGKVPVNINFTAGQDAMSSAIKQCGIKTILTSRLFLTKASLEEMPGMVFLEEVKK
                     TFTPAQKISTAAKAALLPAGYLNRNVTNSATPDDLATVIFSSGSTATPKGVMLSHYNI
                     VSNIEGICQVIQFTKKDRIMGILPLFHSFGFTATIWLPLLAGFGVVYHPNPMDAKTIG
                     ETVQKHQATLLISTPTFYAGYLKRCSREEFASLRYAIAGAEKLREQIAGGYREKYGID
                     LLEGYGCTELAPVVSVNVPDVFDGGEKQIGNKPGTVGHPIPGVAVKVIDPETELPLDC
                     GQEGLLLVKGPNCMLGYL"
     gene            3096..3869
                     /gene="pgeF"
                     /locus_tag="VF089_19355"
     CDS             3096..3869
                     /gene="pgeF"
                     /locus_tag="VF089_19355"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX6176172.1"
                     /translation="MKINWLKVLQWEKDTGLLHGFIGRQGGKSVGAYAGLNVSYRVGD
                     DPKIVSQNVCDVKLAVGIHDGRIVTMKQVHGDRLIEVKDTHLKEAGEADGMVTREQNT
                     FLGVLTADCVPMLFLAREQKIVAVVHAGWRGTLKGIAGKTVKFLLENYGVLPSELEVA
                     LGPSIDACCYEIGRDVAQPLIEQWAELATYSIETRDNKTYLDLRNLNEAILHRAGVSQ
                     SQIFRVGPCTSCAKDDFFSYRREKRETGRQISFIGWQSE"
BASE COUNT         1038 a         1045 c         1126 g          893 t
ORIGIN      
        1 cgagataacc gagcatgcag ttggggcctt taacgagaag caaaccctct tgaccgcagt
       61 ctaggggcag ctccgtctca ggatcgatca ccttcaccgc gacgcccgga atcggatgtc
      121 cgacggtacc aggcttattg ccgatttgtt tttctccgcc gtcgaacaca tccggaacgt
      181 tcaccgacac caccggcgca agttcggtgc agccgtaacc ttcgagcaga tcaataccat
      241 atttttctct ataacctccg gcaatctgtt cacgtaactt ctctgcgccg gcaatggcat
      301 agcgcaggct ggcaaattct tcccggctgc agcgtttcaa atagcccgca taaaaggtcg
      361 gggtgcttat cagcagggtc gcttgatgct tctgcacggt ttctcctatg gtcttagcgt
      421 ccatcgggtt tggatggtaa accacgccga agcccgccag gaggggcagc cagattgtcg
      481 ccgtgaaacc gaacgaatga aataatggca atatccccat gatgcggtct tttttcgtga
      541 attgaattac ctggcagata ccttcaatgt tcgagactat attgtagtgg gagagcatca
      601 ctcctttagg ggtggcggtg ctgccgctgg aaaatatcac tgtcgcgagg tcatcaggcg
      661 tcgcgctatt ggtcacattg cggttcaagt aaccggctgg caacagcgct gctttggctg
      721 cggttgagat tttttgcgcc ggcgtaaatg tctttttaac ttcttccaga aaaaccattc
      781 ccggcatttc ttcgaggctt gccttggtca aaaataatcg cgacgtaagg atggttttga
      841 tgccgcactg cttaatagct gatgacatag catcttgccc ggcggtgaaa ttgatgttga
      901 cgggcacctt gcccgccagc atgatcgcga ggtttgccag cgcgccaccg accgacgccg
      961 gcagcatgac gcctaccatt ctgtctttcc ggcaatgctc gctgacccaa cggcacagca
     1021 acatactgcc gatcaatgtc ttgccatagg taagttcggc cccggtcgtg tcggccatac
     1081 agaaggaaaa ccaccgacgc ttagccattt ggacaaactt ggcgggcaac aaatcggttg
     1141 gtttgcggcg ataccgtcgg gcctcgctag cgagctccag gaccgcctgg cggacttggt
     1201 ttgcggtgga attggcctgc agtggttttc cgaacgagac cgtgaccgga tagggaaaaa
     1261 gcttgggcca tttccagaaa aaccggccgc catgaaagct aaagacgctg ccccagactt
     1321 ggtccaagtg aaccgggata atcggcacac tcgtgccctc gaccaccttt tcgaatccgc
     1381 gcttgaatgg aagaatgtgg ccggtacggg tgatggcgcc ttcggcaaaa atacaaacga
     1441 catgtccggc ctcaagctcc tggcgagctc gtttaaggga attgacgaca tcacggcgat
     1501 gcgttgccct gattggaatc gacttcatca gacgaaaaaa ccagttgagc catttgttct
     1561 catagtagtc gcgatgcagg atgaagcgaa caaaaccagg caggcatgcg ccgatcagga
     1621 gagcatcggc gaatgataca tgatttgaca ccagtagcgc cggagtgcct acgggtgcgt
     1681 tttcctcacc gaccacccgt attctgtaaa gcgtgtgcgt caataaccag gcgacgagcc
     1741 gcacgaaata gtccggcaga accactaaaa caatggccgt ggcgatgagc gtgagtattc
     1801 cgatcaagaa aacgatccag tcgggcgata gttgaagcgc gctatttagg aaccagtgaa
     1861 tggccgcggc caaaataatc ccgatcgtat taaaaaagtt attggtggcg atcagttggc
     1921 ctttttcctc gcggccgctg cggtgttgca gtagcgcgtt gagcggcacc gcgaagagcc
     1981 cgcccgaaaa gcccaacatg acaagagacg ccgaagtata aaaataggat gatgcggaat
     2041 acgacaccag cagtaagcaa aggcccatcg agatcgaccc cagcggcacg aggccgagct
     2101 cgattttgtc gccagagagc ttgccggccg ccaaactgcc gatgccgatg ccgatcgcca
     2161 ggaaagtccc gagcaggctg atgtggagtt catcgagccg cagcaattcc ttgccgaaaa
     2221 acaacatggc gagctggacc aaagctccca ggaaccaaaa atacgatatt ccaataactg
     2281 ttagacggag cggcctatcg ggatagagcc gctttatgcc atcccagatt tcaccgagtg
     2341 gattaagatt gaaacgcttg gtggatccgg atgcgggaac gggatatatt tttagactgg
     2401 tcattgttcc gaggacggcg atcgcgacca tgatcagacc gaccaatggc agctgatcct
     2461 tccagatcga gtaaatcgcc ccgccaagag aaacccccaa gatgatcgcc ataaaggtgc
     2521 tcatctccaa caagccattg ccgcgcgaga gttcagtctc ggggagcatc tcggggagaa
     2581 tgccgtattt tgccgggctg aagaaagctg aatgaaggcc catgagaaaa acgatgacca
     2641 gcataggctc gacgtagccc gacaaaaagg ccgcaaaccc catccccatg atgaggattt
     2701 cgaagcactt caccgtgaca agaattgagc gcttgctgta gacatccgct aaatggccgg
     2761 catacccaga aaaaagcgcg gaaggcagga taaaaaggaa ggcgatcagg tcgacgtata
     2821 agctgtcgct ttgcggggtt gtggcgttga gcgcgaccag agatacgacg atcttataga
     2881 agttgtcgtt gaaggcgccg agaaattgag tgcaaaaaaa agcgaagaag ccggcttgtt
     2941 tgagcgtgcc gatgtatcgt ccgttcgtca tgaagtactg ccgatctacg ggggtatttt
     3001 tcggcgaaat tgagccacaa tgcaagagtg aataacccgc ccgagtttct cccattgact
     3061 tgcgtcgcgc agatgatcca aacataatac ccggtatgaa aatcaactgg ttgaaagtcc
     3121 tgcagtggga aaaggacaca ggtttactgc acggctttat aggccggcag ggtgggaaga
     3181 gtgtgggagc ttatgccggg ctcaatgttt cctaccgcgt cggcgacgac cccaaaatcg
     3241 tcagccagaa cgtttgtgac gtgaaactcg ccgttggaat tcatgatggc cggatcgtta
     3301 ctatgaagca ggttcatggc gatcggctga ttgaggtgaa agacacccac ctcaaagaag
     3361 ccggtgaggc cgacgggatg gtgacgcgcg agcagaatac tttccttggg gtcctcacag
     3421 cagattgcgt gcctatgctt ttcctggcgc gggaacagaa gatcgttgcg gtcgttcacg
     3481 ccggctggcg cggaaccctg aaaggaatcg ccggcaaaac agtcaaattc ttattggaaa
     3541 attatggcgt gctgccttcc gagttagaag ttgccctggg tccgtcaatc gacgcatgtt
     3601 gctacgagat cggtcgtgac gtagcgcagc cgctgatcga acaatgggct gaactggcaa
     3661 cttattctat cgaaaccaga gataataaaa cctaccttga tttgcgtaat ctgaatgaag
     3721 ccattctgca ccgcgccggt gtttcgcaaa gccagatttt tcgagtcggt ccgtgtacca
     3781 gctgcgccaa ggatgatttt ttttcctacc gacgagaaaa aagagaaacc ggtcggcaga
     3841 tcagtttcat cggctggcaa tctgaataaa tttggtctcg aatcgcgctc agaaatttct
     3901 tttgcatgag gaaaaaatcg ccgcgcgtga cacatccctg ttgacagcag acaccttgct
     3961 tcttaaacta aaagtcagca gggaaaggag gtggtccaga catgattgat gataattgtt
     4021 ttgtctgcga ggtgactgag acttaggctt caagtggatc tttgacgggg attctagctt
     4081 gaagcctagc aatccccctc ag
//
LOCUS       DASUTG010000834         9251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_278740, whole genome shotgun sequence.
ACCESSION   DASUTG010000834 DASUTG010000000
VERSION     DASUTG010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278740"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..747)
                     /locus_tag="VF089_19360"
     CDS             complement(196..747)
                     /locus_tag="VF089_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6176173.1"
                     /translation="MNTKHTGNVSEGETAFNKRPVYRFLADDHARLDLLLQRAFADAN
                     QVDLHAYSEFRSGLLKHIAMEEKVLLPAAQQAQDGEPLAIAAKLRIQHGALAALLVPS
                     PTCAIGAALRAILRDHNPLEEGSGGVYEVCERLAGSEAGTMLAKLQSYAEIKLAPHND
                     GPLVMESTRRALARAGYNLDDYS"
     gene            862..2049
                     /locus_tag="VF089_19365"
     CDS             862..2049
                     /locus_tag="VF089_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176174.1"
                     /translation="MAADSAKKTPLIRLEVVKAAAWGIIATLILAMLIVVGSRNLSHF
                     DAALVGYTFATLFAVFGITYRYSMWLQRPPTALYWRRGWQVYFKREHRLVNFKNWCQR
                     VVNDIALNRFILRRGRHRWLAHWLIMWGTIIAAAITFPLVFGWIHFQSVSGEIDRYRL
                     FLFGFPTFTFPVHSTVAFFLFHGLVWSAFLVIAGVMIAMRRRMREQGAAAVQLFAEDL
                     LPLILLFAISVTGLLLTASYSWMKGYAFDFLAIVHAITVIFTLLWLPFGKFFHIFQRP
                     AQLGVGFYKDVGSREEQALCRRCGHPFSSRLHVEDLIGVERELGYRYEMEGNDAHHYQ
                     WICPPCRRAMLAIAQGRIWQQARGAGPSSKPRAQPPAMPSYANPVIGEGPLGEEDQNN
                     FHP"
     gene            2058..4304
                     /locus_tag="VF089_19370"
     CDS             2058..4304
                     /locus_tag="VF089_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX6176175.1"
                     /translation="MATIPVNTIEVIERFGPHLARSTGARLDPGVEPDKAVKTHCCFC
                     GQQCGIELLVKNNEVIGFEPWMDFPFNQGKLCPKGVKRYLQGSHSDRLLHAYERDPSA
                     PGGFKSMDYRRAIERVADAIKRMQSQHGNHAFAVLSGASLTTEKAYLMGKFAHMCLRT
                     SNIDYNGRLCMVSAAGGNKKAFGIDRAANPWSDILKAELVWISGANVADCAPITTDYV
                     WQARENGAKIIVVDPRITPVARTCDLFLPIKPGRDVALFNGILHLMIENDWLDHDFIE
                     QYTAGFDEVARHVAEWTPQKTAEVTGIAERTIRQAAEWWGRAKTSFLMHARGIEHHTH
                     GVQNVLGAINMVLASGRIGRPYCGYATITGQGNGQGGREHGQKCDQLPGARDIWNPEH
                     RAYIAKVWGIEPNEIPEAGVDAYEIFRKIDAGEIKGLLTICFNPVVSLPDNNFIRRML
                     EKLDFYVAIDFFLNETARYADIVLPGSLHEEDEGVVTTAEGRVIKINKAIDCPGEARE
                     DWRIIQDIAGALGRERGFTFNSPGEIFAELREASRGGIADYSGITYEKIERQYGVFWP
                     CPSEDHPGTPRLFEENSWNPVARGNGRFYFPDGKARFNVACYTPPAEDVDDAYPIILT
                     TGRVVSQFLSGTQTRRIGPLVDHYPEPRVEIHPILAERLRIAEGDWITVESRRGDCTL
                     RAQVVTTIRPDTVFIPYHWAGRRSVNQLTISAQDPISKIPEYKVCAVRLKKAEREPDY
                     ASGLEPQQ"
     gene            4330..4962
                     /locus_tag="VF089_19375"
     CDS             4330..4962
                     /locus_tag="VF089_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6176176.1"
                     /translation="MEFFVDPGRCIGCQSCVQACSECDTHKGHSMIQLDYIDRANSTQ
                     TVPVICMHCDSPTCAEVCPADAIKRTGDGVVQSARKPRCIACNNCVIACPFGVPKMNT
                     GMHLMMKCDMCYDRTSVGKKPMCASVCPSQALFFGTREEIEKLRPLSRPINKFQFGNQ
                     TISTRVNLMVPKAAPAEHLDVTAALHEAPVGQDMLDGLLIDNMTADWEEG"
     gene            4959..5507
                     /locus_tag="VF089_19380"
     CDS             4959..5507
                     /locus_tag="VF089_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6176177.1"
                     /translation="MSGRDRDQLTVAPDGRPEADQPRWRQDFPIDWPQDEYIARRDFT
                     KFMVLTSFAFAVGQLWILAQNYLRRSRRALPAREIAAVNDIPVGSSRIFNYPGAHEPA
                     LLVRLDESRFVAYDQRCTHLVCPVIPQIEAGRFYCPCHEGSFDLASGRPLAGPPRRPL
                     TQIRLEIRGGRVFAVGIQERTA"
     gene            5504..5728
                     /locus_tag="VF089_19385"
     CDS             5504..5728
                     /locus_tag="VF089_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HEX6176178.1"
                     /translation="MNDPRFARHQKRTILSGVLCFVMILVILQLWLLTATMNAYLGGD
                     ESVLGPAALASIACLGLNAGLLWYVYALDR"
     gene            5820..6881
                     /locus_tag="VF089_19390"
     CDS             5820..6881
                     /locus_tag="VF089_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX6176179.1"
                     /translation="MTLLSLITASIRDLHPAYFAMVMATGIVSIAAHLLQMTLLAASL
                     AWLNLAAFAVLWLLTLVRLFHHRSRVFADLIDHNRGVGFFTMVAASCVLGSQCLLVLE
                     NSFLARLLWLLGIGLWIVLTYTIFTALTVKEHKPVLETGIHGGWLIAVVATQSVSNLG
                     TLLATQFTAYQPILFFTLSMWLWGGMLYIWMISLIFYRYAFFQMAPSDLTPPYWINMG
                     AVAISTLAGTSLMLSASDWSFLQGIFPFLQGFTLFFWATGTWWIPMLVILGIWRHFYK
                     GFKLTYDPLYWGAVFPLGMYTAATFQLAKAIHLDFLLFIPRYFFYIALLAWLVTFVGF
                     VHNVLRALLDLRLQHAAPR"
     gene            6925..7266
                     /locus_tag="VF089_19395"
     CDS             6925..7266
                     /locus_tag="VF089_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176180.1"
                     /translation="MTIATKNIFERISGAAGAEEFLTLLEENGVKIQRIVSHASSTPE
                     KEWYDQAYPEWVLVVRGHAVLEFEGAERVELNEGDYLTIAPHARHRVDETGPETIWLV
                     VHLKQQTGVET"
     gene            complement(7385..8971)
                     /locus_tag="VF089_19400"
     CDS             complement(7385..8971)
                     /locus_tag="VF089_19400"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6176181.1"
                     /translation="MRFSEFATLCYELEQTSSRLAKVAMAAEYLKRLAPEEIRHGIAF
                     LSGRPFPVSDSRTLDIGPGAFAHASETPELENSVSPELTLTDVADSFSGIAEANGKGS
                     RGAKYARLRELVEMTGSQERPILFRLLHNELRIGLHDGLIQEAIARASDGDLKTVRRA
                     ALFLSDLAEVASIALTQGGAALQEVDIKLFVPLLPMLSELSEDFDSVLAAHGGSSAFE
                     FKYDGARVQIHQSGGQVRIWSRRLTEVTDSLPEIAQIVRSDLLGDSFILDGEVVAVGK
                     DGRPFPFQELMRRFRRIHNIEAAAIDIPLHLHLFDCLLLNGASLIDEPYERRWETLAN
                     ITGGKHLARRHITSDKVLAENFLKDALAAGHEGLMAKALTSPYMPGNRGKLWFKIKPA
                     ETVDCAIIAADRGSGRRRGWLSNYHLAVADGEGGFAPVGKTFKGLTDKQFAEMTARLQ
                     ELQIADDGYTVAVKPEVVVEVAYNEIQHSPQYSSKFALRFARIKRIREDKNVDQITTL
                     TELQNLYERQFVSKSKRTKS"
     gene            9005..>9251
                     /locus_tag="VF089_19405"
     CDS             9005..>9251
                     /locus_tag="VF089_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176182.1"
                     /translation="MARAVLTPAVRVMIDSRFVKGNMAMGKGLAMMTRRKLLQAAIAA
                     GIWHLLGGVGRAGQALSMKDIENMQKNWRSLLAKDFKA"
BASE COUNT         1870 a         2589 c         2676 g         2116 t
ORIGIN      
        1 ggcgggattc tcgggcatca aaggaccttt tcgctcgagc ccgctatcct ttgatcccga
       61 ctccgtattg atcgcaaaga agagctgaat agggatttca agagtgcgga ctccatcgct
      121 ggggctgtgt tcgtcggcgt gcaccatttc aacttttcta tttctgtaat gctgtctctt
      181 gagatgatct atgcatcagg agtaatcgtc caggttatag cccgctctgg ccagcgcgcg
      241 gcgtgtggac tccatgacta gtggcccatc gttgtgcggc gccagcttga tctcggcata
      301 ggactgcagc ttggcaagca tggtaccagc ttcggacccc gccagtctct cgcaaacttc
      361 atagactccg ccagaacctt cctcgagagg attgtggtcc ctcaggattg cgcgaagtgc
      421 cgcgccaatt gcgcacgtcg gcgacggaac aagaagcgcg gccagcgcgc cgtgttggat
      481 gcgcaacttc gcggcaatcg ccagcggttc tccatcctgc gcctgctgag ctgccgggag
      541 taatactttt tcctccatcg cgatgtgttt gagcaagcct gaacgaaact cactgtaagc
      601 gtgtagatca acctggttgg catcggcaaa ggcacgctgc agtagcagat ccaggcgagc
      661 atgatcgtcc gctaggaacc gatatacagg acgtttattg aatgcagttt ctccctcgga
      721 gacatttcca gtgtgtttag tgttcatgat ttggtcgttt aagtatagtc atttgacttc
      781 taaaatctaa tcctcggtta aaatgacagt gatgacttca ctcgaagatc attaagatgt
      841 agtgtcttct tgagaaaaaa catggctgcc gattctgcga agaagacacc gttgataaga
      901 ctcgaagtcg tcaaagccgc ggcgtggggg atcattgcga cattgatact ggcgatgctg
      961 attgtcgtcg gatcgcgcaa cctgtcgcac ttcgatgcgg ccctggtcgg ctacacgttc
     1021 gccacgctat ttgccgtttt cggtatcacc tatcgctatt ccatgtggct gcagcggccg
     1081 ccgacggcgc tgtactggcg gcgcggctgg caggtttact tcaagcgcga gcaccggctc
     1141 gtgaacttca agaactggtg tcagcgcgtc gtcaacgaca ttgcattgaa tcgtttcatc
     1201 ctgcgtcgcg gccgccaccg ctggctggcg cactggttga ttatgtgggg tacgatcatc
     1261 gctgccgcca tcacgttccc gctggtattc ggctggattc actttcagtc ggtgagcggt
     1321 gagatcgacc gctatcggtt gtttctcttc ggctttccca cattcacgtt cccggtccat
     1381 tccaccgtgg catttttcct gtttcacggc ttggtgtggt cggcctttct ggtcatcgcc
     1441 ggagttatga tcgccatgcg ccggcgcatg cgcgagcagg gggcggcggc cgttcaattg
     1501 ttcgcggaag atctgctgcc gttgattctg ctcttcgcca tcagtgtcac cggcttgtta
     1561 ttgaccgcga gctatagctg gatgaaaggc tatgcgttcg atttcctcgc catcgtccat
     1621 gccatcaccg tgatctttac gcttctttgg ctgccgttcg gcaagttctt ccacatcttc
     1681 cagcgaccgg cgcagcttgg cgtcggtttc tataaagatg tcggttcgcg cgaagagcag
     1741 gcgctgtgtc gccgttgcgg tcatccgttc agctcgcgac tgcacgtcga ggatctgatt
     1801 ggagtagagc gcgagttggg ctaccgctat gagatggaag gaaatgatgc gcaccattat
     1861 caatggatct gcccgccgtg ccgtcgcgcg atgctcgcca ttgctcaagg gagaatctgg
     1921 caacaggcgc gcggcgctgg gccttcgtct aagccccggg cgcagccgcc agcgatgcca
     1981 tcgtatgcca atccggtaat cggcgagggc ccgctcggcg aagaagatca gaacaatttt
     2041 catccgtaaa gcattccatg gcgacaattc ccgttaacac gattgaggtg atcgaacgct
     2101 tcgggccgca tttggcgcgc tccacagggg cccggttgga tccgggcgtg gagccggata
     2161 aagcggtgaa gacgcattgc tgtttctgcg gccagcagtg cggtatcgaa cttttagtca
     2221 agaacaacga ggtcatcggc ttcgagcctt ggatggactt tcctttcaac cagggaaagc
     2281 tctgccccaa aggcgtgaag cgctatctgc aaggatccca ttcggatcgg ctgttgcacg
     2341 cttacgaacg cgatccatcg gcgccaggcg gcttcaagtc gatggactat cgccgcgcta
     2401 tcgagcgtgt cgccgatgcg atcaagcgca tgcagtcgca gcacggcaat catgccttcg
     2461 ccgtgctaag cggcgccagc ctgacaaccg agaaggctta tctcatgggc aaattcgcac
     2521 atatgtgcct gaggacctcg aatatcgatt acaacggtcg cctgtgcatg gtcagcgccg
     2581 ccggcggcaa taagaaagcc tttggcatcg accgtgcggc caatccatgg tcggatattc
     2641 tcaaagctga actggtctgg atcagcggcg ccaacgtagc ggactgcgct cctatcacca
     2701 ccgattacgt gtggcaggcg cgcgagaatg gcgccaagat tatcgtcgtc gacccgcgca
     2761 ttacccccgt ggcccgcacc tgcgatctgt tcctgccgat caagccgggg cgagatgtag
     2821 cgctcttcaa tggcattctt catcttatga tcgagaacga ctggctcgac catgacttca
     2881 tcgaacaata cactgccggc tttgacgagg tggccaggca tgtcgcggag tggacgccgc
     2941 agaaaacggc cgaggtcacg ggtattgcgg aacgaaccat ccgacaagcg gccgagtggt
     3001 ggggcagagc caaaaccagc tttttgatgc acgcccgcgg tatcgaacac catacgcacg
     3061 gtgtgcagaa cgtgctcggc gcgatcaaca tggtgttggc ttccggccgt atcggacgcc
     3121 cgtattgcgg gtatgccacc atcaccgggc aaggcaatgg gcagggcggc cgcgagcacg
     3181 gtcagaaatg cgatcaactc ccgggcgcgc gcgatatctg gaacccggaa caccgcgctt
     3241 acatcgccaa ggtctggggc atcgaaccga acgagatccc agaagccggc gtcgatgcct
     3301 acgaaatttt ccgcaagatc gacgccggtg agatcaaagg acttttgacg atttgtttca
     3361 atccggtggt ttcgctgccc gacaataatt tcatcaggcg catgctcgag aagctggatt
     3421 tctatgtggc gatcgatttc tttttaaacg agaccgctcg ttacgccgat attgtgctgc
     3481 cgggatcgct ccacgaagaa gacgaaggcg tggttaccac cgcggaggga cgggtaatca
     3541 aaataaacaa ggcaatcgac tgccctggag aagcgcgcga ggactggcgc atcattcagg
     3601 atatcgccgg cgcactcggc cgcgagcgcg ggtttacctt caatagcccc ggagaaattt
     3661 tcgccgaatt gcgcgaagct tcgcgcggcg gcattgcgga ttactcgggc attacgtacg
     3721 aaaagatcga gcgccaatat ggagtgttct ggccttgtcc gagcgaagat catcccggaa
     3781 cacccaggct gttcgaggaa aactcatgga atccggttgc ccggggcaac ggtagatttt
     3841 attttcccga tggcaaagcc cgcttcaacg tggcgtgtta cacgccgcca gccgaagacg
     3901 tcgatgacgc atatccgatc atcctgacca ccggccgggt cgtcagtcag tttctctccg
     3961 gcacgcagac gcggcggatc ggccccctag tggatcatta tcccgaaccc cgtgtggaaa
     4021 ttcacccgat actcgccgaa cgtttgagaa ttgccgaagg cgattggatc accgtcgaat
     4081 cgcgccgcgg cgactgcact ctgagggcgc aggtggtcac gacgattcga ccggataccg
     4141 tttttattcc ctaccactgg gcaggacggc gcagcgtcaa tcagctgacg atctccgcgc
     4201 aggatccgat ttcgaaaatc cctgagtaca aggtgtgcgc ggttcgattg aaaaaggcgg
     4261 agcgggagcc ggactatgcg agcggtctcg agccgcagca gtagcagaat gatgcctgtt
     4321 ccagcacaga tggagttctt cgttgacccg ggccgctgta tcggctgtca atcctgcgtg
     4381 caggcttgca gcgagtgcga cacgcacaag ggtcactcca tgattcaatt ggattacatc
     4441 gaccgggcga actcgaccca aacggtgccg gtgatctgca tgcattgcga ttcaccgacc
     4501 tgcgccgaag tttgccccgc cgatgcgatc aagcgcaccg gcgatggcgt ggtacaaagc
     4561 gcgcgcaagc cgcgctgcat cgcgtgcaat aactgcgtga tcgcctgccc gttcggcgtg
     4621 cccaagatga ataccggcat gcatctgatg atgaagtgcg acatgtgcta tgaccgcacc
     4681 tcggtcggca aaaagccgat gtgtgcctcg gtctgtccca gccaggctct gttcttcgga
     4741 acccgcgaag aaatagaaaa gctccggccg ctctcgcggc cgatcaacaa atttcaattc
     4801 ggcaaccaga cgatcagcac gcgggtcaac ctgatggtcc ccaaggcagc gccggctgag
     4861 catctcgacg tcaccgccgc gcttcacgaa gcgccggtcg ggcaagacat gctcgacggt
     4921 ttgctgatcg acaacatgac agccgattgg gaggaagggt gagcgggcga gatcgcgacc
     4981 agctcacggt ggcaccggac ggcaggcccg aagcggacca gccgcgatgg cgccaggatt
     5041 tccccataga ctggccgcag gacgagtaca tcgcgcggcg cgacttcacc aaattcatgg
     5101 tgctgacaag cttcgctttc gccgtggggc aattatggat tctggcgcaa aattatctcc
     5161 ggcgcagcag gagagccttg ccggcgcggg aaatcgcggc cgtcaatgat attccggtcg
     5221 gatcttcacg gattttcaat tacccgggcg cgcacgagcc ggcgctgctg gtgcggctgg
     5281 atgagtcgcg ctttgtcgct tacgatcaaa ggtgcacgca cctggtatgc cccgtgattc
     5341 cgcaaatcga agccgggcgc ttctactgtc cttgtcatga aggctcgttt gatctggcca
     5401 gcgggcggcc gctggcgggt cctccgcgcc ggccgctgac gcagatccgg ctggagattc
     5461 gcggcgggcg agtttttgca gtcggtatcc aggagcgtac ggcatgaacg atccgcgctt
     5521 cgcgcgccat caaaaaagga ccattctctc cggcgtcctg tgctttgtca tgattttagt
     5581 tattctccag ctgtggctgc tcacggccac aatgaatgcc taccttggcg gcgacgaatc
     5641 ggttctcggt ccggcagccc tggccagtat tgcctgtctc gggctcaatg ccgggttgct
     5701 ctggtacgtc tatgctttgg atagatgact gccgagacat ctcttgccat cagcagtgac
     5761 aagcgcttgc ttccgcccac tgcacggtcg atcttcttcg tgacggtgaa acatagctca
     5821 tgactctgct cagtttgatc acggcatcga tcagagatct gcacccggca tactttgcca
     5881 tggtcatggc caccgggatc gtctccattg ccgcgcatct gttgcagatg acgttgctcg
     5941 ccgccagcct ggcctggctt aacctggcgg cgttcgcggt tctctggctg ttgaccttgg
     6001 tgcgcctgtt tcaccacaga tcgcgtgttt ttgcggatct gatcgatcac aatcgcggcg
     6061 tcggtttctt caccatggta gcggcatcgt gcgtcctggg aagccaatgc ctgctcgtgc
     6121 tggaaaattc gtttctggcc cggcttctct ggttgttagg aataggtctt tggattgtgc
     6181 tgacctacac gatatttacc gccctgaccg tcaaggaaca caaaccggtt ctggagactg
     6241 gaatccatgg cggctggctc attgcggtgg tggcgacgca gtctgtttcc aacttgggaa
     6301 cgttgctcgc gacgcaattc actgcttatc aaccgattct cttctttacc ttatcgatgt
     6361 ggttgtgggg cggcatgctc tacatatgga tgatctcttt gatcttctat cgctatgcct
     6421 tttttcagat ggcgccttcg gacctcaccc cgccgtactg gatcaacatg ggcgcggtgg
     6481 cgatctctac tttggcggga acgagcctga tgctcagtgc atcggattgg tcgtttctcc
     6541 agggaatctt tccctttctt cagggcttta cgcttttttt ctgggccacc gggacgtggt
     6601 ggattcccat gcttgtgatt ctcgggatct ggagacactt ctacaaggga ttcaagttga
     6661 cgtatgatcc tctctattgg ggtgccgtgt ttcctctcgg catgtatacc gcggcaacct
     6721 tccagctagc caaggcaatc catttggact tcctgctttt tattccgcgc tactttttct
     6781 acatcgctct gttggcatgg ttggtcacgt tcgtcgggtt cgtgcacaac gtgctgcgcg
     6841 ctctgctcga tttgcgcctc caacacgcgg cgccacgctg attagcatac cgtacagggg
     6901 gcctgccaat cgccggagat tattatgacg attgcaacca aaaatatctt cgaacggatc
     6961 tctggagccg ccggtgcgga agagtttttg accttgcttg aggaaaacgg cgtcaaaatt
     7021 cagcgcatcg tttcccatgc aagcagcaca ccggagaagg agtggtacga tcaggcgtat
     7081 cctgaatggg tgctggtggt tcgcggccat gcagttctgg agtttgaggg cgccgagcgg
     7141 gttgagttaa atgaagggga ttacctgacc attgcaccgc acgcgcggca tcgggtcgat
     7201 gagaccgggc ctgaaacgat ctggctggtg gtgcatctaa aacagcagac cggagtggaa
     7261 acctaagttc cagccttcca aatgttccaa tcgttccaaa tgtctaccga agaatatctc
     7321 agccgtttac ggatttcagt tgctggaacc actggaacga ttggaactgt tggaacattt
     7381 ggaactaaga ttttgttcgt ttgctcttgc tgacaaattg ccgctcgtaa agattctgca
     7441 gttcagtcaa cgtggtgatt tggtcaacgt tcttatcctc ccggattcgc ttgattcggg
     7501 cgaagcgcag ggcgaacttg gagctgtatt gcgggctgtg ctggatctcg ttatatgcca
     7561 cctcgacgac cacctccggt ttgacggcga ccgtatagcc gtcatcggcg atctgtaact
     7621 cctgaagccg cgcggtcatt tcggcaaatt gcttgtcggt caggcctttg aatgttttcc
     7681 ccaccggcgc gaacccgccc tcgccatcgg ctacggcgag atggtaattg gacagccagc
     7741 cgcgcctgcg acccgagccg cggtcggctg cgataatcgc gcaatcgacg gtttccgccg
     7801 gctttatctt gaaccagagc ttgcctcgat tgcccggcat gtaggggcta gtcagagctt
     7861 tggccatcaa tccttcatgc cccgccgcaa gagcgtcttt gagaaagttc tccgccagaa
     7921 ccttgtcaga agtaatgtga cggcgcgcca gatgcttgcc gccggtgatg ttagcgagcg
     7981 tttcccagcg gcgctcatag ggctcgtcga tcaatgaggc gccgttcagg agcaggcaat
     8041 cgaacaggtg taagtgcaaa ggaatgtcga tggcggcggc ttcgatgttg tggattcgtc
     8101 tgaaacggcg catgagttct tgaaacggga acggccggcc gtctttgccc accgcgacca
     8161 cctcgccgtc gagaatgaaa gagtcgccaa gcagatcgct gcgcacgatc tgcgcgattt
     8221 ccggcaggct gtcggttact tcggtaaggc gccggctcca gattcgtact tggccgccgg
     8281 attgatgaat ttgcactcgc gcgccgtcat atttgaattc gaaagcgctg ctgccgccgt
     8341 gagcggcaag cacgctatcg aaatcttccg ataattctga cagcatcggc agaagcggca
     8401 cgaacagctt gatgtcgact tcctggaggg ctgcgccgcc ctgcgttagc gcgatcgatg
     8461 ccacctccgc cagatcagat aggaaaagcg ccgcgcgccg caccgtcttt aagtcgccat
     8521 ccgaggcgcg ggcgatcgct tcctgaatca agccgtcgtg cagcccgata cgtagctcat
     8581 tgtgcagaag gcgaaagaga atgggtcgtt cttggctgcc ggtcatttcg accagctctc
     8641 tcaggcgcgc atacttcgcg ccgcgggatc ctttgccgtt ggcctcggca ataccagaga
     8701 agctgtcggc aacgtctgtg agtgtcaact ccggcgagac ggagttttca agttccggag
     8761 tttcggaggc gtgagcgaat gcacccggcc cgatatctaa cgtgcgcgaa tcggataccg
     8821 gaaacgggcg tccggacaaa aatgcgatac catggcgaat ctcctcgggg gcgagtcgct
     8881 taagatattc cgcagccatg gcgacctttg ccaaccggct cgaggtctgc tccagctcgt
     8941 aacaaagggt ggcaaactca gaaaacctca tacggatagt ttaacatgcg gcgcgtcttg
     9001 aaaaatggcc cgcgccgtac ttacgcccgc tgttcgtgtt atgatcgatt ccagattcgt
     9061 taagggtaat atggcaatgg gaaagggact cgccatgatg acaagacgaa aactattaca
     9121 agctgcgatt gctgcaggta tttggcatct gcttggagga gttggacgcg ccgggcaggc
     9181 attgtctatg aaggatatcg agaacatgca aaagaattgg cgctcgttgc ttgccaagga
     9241 tttcaaagct c
//
LOCUS       DASUTG010000835         7353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1020986, whole genome shotgun sequence.
ACCESSION   DASUTG010000835 DASUTG010000000
VERSION     DASUTG010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020986"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..2700
                     /locus_tag="VF089_19410"
     CDS             190..2700
                     /locus_tag="VF089_19410"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6176183.1"
                     /translation="MRHSSGITTWEDFVRTARARLDSLRPSGGAGLRLLTGNVTSPSL
                     GEQIRTLLKQFPQAKWHAYEPVSRDNTREGTRLALGQEAESLYRFDRADVILSLDCDF
                     LFTEPGSLRYARDFSNRRRVRQEKAEMNRLYVAEPTPSITGSMADHRLAITAGRIRSL
                     AEAVARRLGAKQRSAEDAQELAPYQTWIDTVAADLQQHRGSSLVVAGYYQPPPVHALA
                     HSMNQLLGNVGNTVIHAEPIEESAGASSLQELARDMAQGAVQALFIIGANPAYSAPAD
                     LRFAEALAKVRFRAHFAEYEDETSALCHWHVPQAHFLETWGDARSFDGVVTIMQPLIA
                     PLFGGRSTHELFAALTGSPETSAYDIVRGFWRSRSGAADFDRWWQTALHDGFIPDSGA
                     KLKTAAARTQTASSGEADSPAAKPVEGLELVFRPDPTIWDGRFANNAWLQELPKPLTK
                     VTWDNAALISPRTAQRLTLHNGDVVELRLGDSSIAAPVWINPGQADESVTVHLGYGRS
                     RAGKVASAIGFNAYPLRTTGQPWFAGGLQIKKTGRRHELVTTQGHHTMAGRPLVRSGT
                     LEQFREHPDFVHEMGHEPPPDLSLYPEHKFDGSAWAMVIDTTVCIGCNACVVACQAEN
                     NIPVVGKDEVARGREMLWLRVDRYYQGPAENPDVLFQPVPCMHCENAPCEPVCPVAAT
                     VHSTEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFYQYADFRTEVRKLGYNPDVTVRSR
                     GVMEKCTYCVQRINHGRIEAKKENRPIRDGEVITACAQACPTEAIIFGDMTDADATVS
                     KLKKEPLNYAMLAELNTRPRTTYLARLKNPNPDLGVKR"
     gene            2709..4073
                     /gene="nrfD"
                     /locus_tag="VF089_19415"
     CDS             2709..4073
                     /gene="nrfD"
                     /locus_tag="VF089_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6176184.1"
                     /translation="MAETRTRDTRYDVIGPGHTPGSITDKISEIVLTRRTPKGWWIGF
                     GISFLLMNLLFVAVANLLITGIGIWGVNVPVAWGFAIINFVWWIGIGHAGTLISAILL
                     LLRQRWRTSINRFAEAMTLFAVACAGLYPILHLGRPGFAYWLFPNPNTMGLWPQFRSP
                     LVWDVFAVSTYATVSLLFWYIGLIPDLATLRDRARHRFARMAYGMLAMGWRGSAVHWY
                     RYETAYLLLAGLATPLVISVHSIVSLDFAVSVLPGWHSTIFPPYFVAGAIYAGFAMVL
                     TLAIPLRAAYRLEDLITMHHLDNMAKVMLTTGLFVFYGYVMEAFFAWYSANPNEAFMM
                     SNRATGPYAPFFWLLLICNGLVPQLLWSPRLRASVPLLFVISLVVSLGMWLERYIIVV
                     TTLHRDFLPSSWGMYSGTIWDWATFIGTIGLFLSLLFLFIRVLPMISIFEMRELVHEE
                     REES"
     gene            4104..4613
                     /locus_tag="VF089_19420"
     CDS             4104..4613
                     /locus_tag="VF089_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX6176185.1"
                     /translation="MAEFDNPEMLRDGAERAHAAGYRRMDAYSPFPVEGLAEVIGFKR
                     THLPLIVLIGGILGGIGGFYLQYWSSVIDYPLIVGGKPYNSWPAFIPVTFETTILGAA
                     LAAVLGMLGLNGLPMPYHPVFNVERFALASRDRFFLCIEAADPKFDLDNTVEFLRSLN
                     PSSIHEVEP"
     gene            4679..5236
                     /locus_tag="VF089_19425"
     CDS             4679..5236
                     /locus_tag="VF089_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6176186.1"
                     /translation="MMLTAVAAVVLAGCRQQMADQPHKRPLEPSNFFDDGMASRPVLP
                     GTVARAVEEPNNQRLNSKAEGKLVDAFPFEVTMEVLARGQERYEIFCSPCHDRLGTGQ
                     GMIVRRGFTPARSFHEPRLRDAPTGHFFEIITQGFGPMPSYADQLSEHDRWAVIAYIR
                     ALQFSRNVRLDQLPPKDRAKMKDSK"
     gene            5233..6405
                     /locus_tag="VF089_19430"
     CDS             5233..6405
                     /locus_tag="VF089_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176187.1"
                     /translation="MTDSGLDTDERTEGVARLAFIAGGAAALISVIGGFFDSAQFFRS
                     YVVAYIFWLAIPLGSLAFLMLHFLVGGRWGVMIRRLLESATRTLPLIALFFVPLLFGL
                     HAVYPWASAGHIAEDEILRQKSAYLNVPFFIARTILYLALWGVLGHFLSRWSRQQDAS
                     AELPAGKLMRRLSGPGLLIYLFTMSFASVDWIMSIEPHWYSTGYGVIWIINQGVTALA
                     FAIIGLAWLGTREPMAARLRASHFQDLGNLLLAFIMLWAYMMFSQYLIIWSGNLAEEI
                     PWYIARTEGLWRWLPPLLVIFHFFVPLFLLLMRDIKRNRNSLVWVAAGVLVMRLLDTY
                     WMIVPGFEHSALHWLDASLMIGMGGLWLGVFLWQLQKMPLLPLHVALLDEETNAHG"
     gene            6398..6805
                     /locus_tag="VF089_19435"
     CDS             6398..6805
                     /locus_tag="VF089_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176188.1"
                     /translation="MADTEDVKYETEDFSPRGIRWFTAGLAVLAVMALVLMGGLLWFL
                     SGGWPPRQGIVSPMVPAPNMSPEPDLQVASSRDYQEMHAAEEAQLHSYRWVNREAGIA
                     AIPITRAMEILASRGMPAQKSQPKSTQSKQEQP"
     gene            6802..>7353
                     /locus_tag="VF089_19440"
     CDS             6802..>7353
                     /locus_tag="VF089_19440"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX6176189.1"
                     /translation="MKSGLWREFSAAIFALTMSHIMAPEPARSHEDKVAPIAAHNQPE
                     IFKNVGIEQKLGTQLPLETTFRDQTGTSVSLQGYLSEKPAVLVFSYFDCPMLCPLVLE
                     GLVRSLKPLSLDVGRDFDILVISIDERDGPETARKKQLEIVNRYGRDGSDVGWHFLTG
                     EKSAIEKVTQSAGFTYAWDEASKQ"
BASE COUNT         1434 a         2159 c         2129 g         1631 t
ORIGIN      
        1 gtcaagccgc tcgtttacgc cagcgcattc acatagtcag gagttgccac gggcatttta
       61 gtcaagaact atgaaggcag gccgatcaag attgagggca acccgcagca ttcggcgagt
      121 ctgggcggca ccaacccgtt catccaggcg tctatcttaa ccctttatga tccggaacgt
      181 tctcaagtcg tgcgccactc gagcggcatc actacctggg aggacttcgt ccgcacggcg
      241 cgcgcgcggc ttgactcttt gcgtccctca ggaggagcgg gactgcgatt gctcacaggc
      301 aacgtgacct cgccgagttt gggagaacag attaggactc tgttaaaaca attcccgcag
      361 gcaaagtggc acgcctacga accggtgtcg cgcgacaaca ctcgtgaagg aacccggctg
      421 gctctcggac aggaagccga gagcctctac cgtttcgatc gcgccgacgt gattctctcc
      481 ctggattgcg attttctctt caccgagccc ggctcgcttc gctatgcgcg tgatttctcc
      541 aaccgcagaa gagtgcgcca agagaaggcg gaaatgaacc ggctctatgt tgccgaaccg
      601 acgccgtcga tcaccggatc catggcggat catcgcctcg ccataaccgc cggccggatt
      661 agatcgttag ccgaggcagt cgcgcgacgc ctcggcgcca aacagcgcag cgccgaggat
      721 gctcaagaac ttgcgccgta ccaaacctgg atcgacacgg ttgccgcgga cctgcagcag
      781 catcgcggct cgagcctggt tgttgcaggc tattatcaac cgccgccggt gcatgctcta
      841 gcccactcga tgaaccagct ccttggcaac gtgggaaata cggtcatcca tgccgagccc
      901 atcgaggaat cagccggcgc aagctcgctg caagagctgg cgcgcgacat ggcgcaaggc
      961 gcggtccagg cgctgtttat catcggtgcc aatccagctt acagcgcgcc ggcggatctg
     1021 cgtttcgccg aggcgctggc caaagttcgc ttccgcgccc atttcgccga atacgaagat
     1081 gagacatcag cgctgtgcca ctggcatgtc ccgcaggctc atttcttaga gacctggggc
     1141 gatgcccgga gtttcgacgg ggtcgtaact atcatgcagc cgttaatcgc gccgctcttt
     1201 ggcggcagat cgacgcatga gctgttcgcg gcgcttaccg gtagcccgga gacatccgcg
     1261 tatgacatcg tgcgcggatt ctggcgcagc cgcagcggcg cagcagattt cgatagatgg
     1321 tggcaaaccg ctttgcatga cgggtttatc cccgatagcg gcgcgaagct caagacggcc
     1381 gccgctcgaa cgcaaactgc atcttcgggt gaggcagatt ctcctgcggc aaagccggtc
     1441 gaaggtctcg aattggtatt tcgccccgac ccgacgatct gggacggtcg ctttgccaat
     1501 aacgcgtggc tgcaggaatt acccaaacct ttgaccaagg tgacatggga caatgcggcg
     1561 ttaattagcc cgcgcacggc gcaacgattg accttgcaca acggcgacgt cgtcgagctc
     1621 cgcttgggcg attcttccat cgccgcgccg gtttggatca accctggcca agctgatgaa
     1681 tcggttacgg tgcatctagg ctacggccgg agccgcgccg gcaaggtcgc cagcgcaatc
     1741 ggcttcaatg cttatcccct gcgtaccacg ggacagccat ggttcgccgg cggtttgcag
     1801 atcaaaaaga ccggcagacg tcatgagctc gtcaccactc aaggtcatca tacaatggca
     1861 ggacgaccgc tggtccgcag cggcacactc gagcagttcc gcgagcatcc ggatttcgtt
     1921 cacgagatgg gccatgaacc gccgccggac ttgtctctct atcccgaaca caagttcgac
     1981 ggcagcgcat gggcgatggt catcgatacg accgtatgca tcggttgcaa tgcgtgcgtt
     2041 gttgcctgcc aggcggaaaa caacattcca gtcgtcggca aagacgaggt tgccagaggc
     2101 cgcgaaatgc tgtggttgag agtcgatcgg tactatcagg gcccggcgga aaacccggac
     2161 gttctgttcc agcccgtgcc gtgcatgcac tgcgagaatg cgccgtgcga accggtctgt
     2221 ccggtagccg ccacggtgca cagcaccgag ggattaaacg acatggttta caaccgctgc
     2281 gtcggcaccc gctactgttc caacaactgt ccttacaaag tgcggcgctt caatttctat
     2341 caatatgccg atttccgcac cgaggtgcgc aagctgggtt acaaccccga cgtcacggtc
     2401 agaagccgtg gcgtgatgga aaaatgcacg tattgcgtgc agcggatcaa ccacgggcgc
     2461 atcgaggcaa aaaaggaaaa tcggccgatc cgcgacggcg aagtcatcac cgcctgcgcg
     2521 caggcttgtc ccaccgaagc gatcatcttc ggcgacatga ccgatgccga cgcgaccgtt
     2581 tccaaactca aaaaggaacc gctcaactac gccatgctgg ccgaacttaa cacccggccg
     2641 cggacaactt atttggcacg gcttaagaat cccaatccgg acctgggagt taagaggtga
     2701 cggaccccgt ggccgaaacc agaacacggg atacccgcta cgatgtgatc ggccccggtc
     2761 ataccccggg ttcgatcacc gacaaaatca gcgagatcgt tctcacccgc agaacaccaa
     2821 agggctggtg gattggcttc ggcatttcgt ttctgttgat gaatctcctg tttgtcgcgg
     2881 tcgccaatct tttgattacc ggcatcggca tctggggcgt caatgtcccg gtggcatggg
     2941 gctttgcgat tatcaatttc gtctggtgga ttggtatcgg ccatgcaggc actctgatat
     3001 cggcgatcct cctgctgttg cgacagcgct ggcgtacttc gatcaaccgc ttcgccgaag
     3061 cgatgacgct tttcgccgtg gcgtgcgcgg gactctaccc gattctccat ctgggacgtc
     3121 cgggatttgc ttattggctg tttcccaatc ccaacaccat gggcctgtgg ccgcagtttc
     3181 gcagcccgct ggtgtgggat gtttttgcgg tatccaccta cgccaccgtt tctctgctct
     3241 tctggtacat cggcctgatc cccgacctgg cgaccttgcg cgatcgtgcc cggcaccgct
     3301 ttgcgcgcat ggcctacggc atgttggcga tgggctggcg cggttcggcg gttcattggt
     3361 atcgctatga aaccgcctac ctgctgctgg ccggcttggc tacgcccctg gtgatctcag
     3421 tccacagtat cgtcagcctg gattttgccg taagcgtttt gccgggctgg cattcgacaa
     3481 ttttcccgcc ctatttcgtt gccggagcga tctacgccgg ctttgccatg gtgctgaccc
     3541 tggcaattcc gttacgtgca gcctaccggc tcgaggatct gatcaccatg caccacctcg
     3601 acaacatggc caaggtgatg ctgaccaccg ggctgttcgt cttttacggc tatgtcatgg
     3661 aggcgttctt tgcctggtat agcgccaacc ccaacgaggc cttcatgatg tccaatcgcg
     3721 ccaccgggcc gtatgcgcct ttcttttggc tcctgttgat ttgcaatggc ctggtgccgc
     3781 aactcctgtg gtcgccgcgg ctgcgcgcct cggtgccgct gctatttgtt atctctctgg
     3841 tggtcagcct gggtatgtgg cttgaacgtt acatcatcgt ggtcaccacc ctgcatcgcg
     3901 attttcttcc ctcatcctgg ggcatgtatt cggggacgat ctgggactgg gccacgttta
     3961 tcggcaccat cggtcttttc ctctcgttgc tcttcctgtt tattcgggtg ctgccgatga
     4021 tctcaatctt cgagatgcgg gaactggtgc atgaggaacg ggaggagagc tgagtgaaac
     4081 agccgcctat tatctacggc gtgatggccg aattcgataa tccggagatg ctccgtgacg
     4141 gcgccgagcg cgcccacgcc gccggctacc ggcggatgga tgcgtacagc ccgtttcccg
     4201 tcgaagggtt ggccgaagtg atcggcttca aacgcacaca tttaccgttg atcgtgctga
     4261 tcggtggcat tctcggcggc atcggcggct tctacctgca gtactggtca tcggtcatcg
     4321 attatccgct gattgtcggc ggcaagccgt ataacagctg gcccgccttc attccggtca
     4381 cattcgagac gacgattctg ggcgccgcgt tggccgcggt tctgggcatg ctcggattga
     4441 acgggttgcc gatgccgtat catccggtat tcaacgtcga gcgcttcgcg ctggccagcc
     4501 gcgaccgttt tttcctatgc atcgaggcgg cggacccgaa gttcgatctc gataacaccg
     4561 tcgaattttt gcgcagctta aacccgagct cgattcatga agtggaacca tgacgaattg
     4621 aacccgtagc tggttcagcg ctcaatcgtt cattatgttg aaactgttga aaaagttgat
     4681 gatgcttacg gctgtggcag cagtcgtgct tgccggatgc cgccaacaaa tggcggatca
     4741 gccgcacaag cgaccgttag agccgagcaa cttttttgat gacggcatgg cttcacggcc
     4801 ggtcttgccc ggaaccgtgg cgcgcgccgt ggaagagccg aacaaccagc ggctcaatag
     4861 caaagccgaa ggcaaactcg tcgatgcctt cccctttgag gtaactatgg aagtgctggc
     4921 gcgtggccag gaacgctatg aaatattttg ctcgccgtgt cacgatcggc tgggcaccgg
     4981 ccagggaatg atcgtccggc gcggttttac accggcgcga tcctttcacg agcccaggct
     5041 gcgcgatgcc ccgacaggcc acttcttcga aatcataacc caggggtttg ggccgatgcc
     5101 ctcgtatgca gatcagctct cggagcatga tcgctgggct gtcattgcct acattcgggc
     5161 ccttcaattc agccggaatg tgcgcctgga tcaattgccg ccgaaagatc gcgcaaagat
     5221 gaaagacagc aaatgaccga ctctggacta gacaccgacg agagaaccga aggcgtggcg
     5281 cgcctggctt ttatcgccgg cggcgccgcc gcccttatct ccgtcatcgg cggcttcttc
     5341 gatagcgcgc aattctttcg ctcgtacgtc gtcgcttata tcttttggct ggcgattccg
     5401 ctgggctctt tggcctttct catgctgcac tttctcgtcg gcggcagatg gggcgtgatg
     5461 atccggcgcc tgctcgaatc ggcaacccgc actttgccgc ttatcgcgct gtttttcgtc
     5521 ccgctcctgt tcggacttca cgccgtttat ccttgggcct cggccgggca tatcgccgaa
     5581 gacgagattc tgcggcaaaa atccgcttac ctgaacgttc cattttttat cgccagaaca
     5641 attttgtatc tcgccctctg gggggttttg ggccactttt taagccgctg gtcgcgccaa
     5701 caagatgcat ccgcggaact gccagcaggc aagctcatgc gccgccttag cggtcccggg
     5761 ttgctgatct atcttttcac aatgagcttt gcatcggtgg actggatcat gtccattgaa
     5821 ccgcactggt attcgacggg atatggcgtc atctggatca tcaaccaagg agtcaccgcg
     5881 ctggccttcg ccatcatcgg gctggcatgg cttggcacgc gcgaaccgat ggcagcgcga
     5941 ctgcgcgcca gccattttca ggatctgggc aatctgttgc tcgccttcat catgctttgg
     6001 gcctacatga tgttttcgca atatctgatt atctggtcgg gcaatctggc cgaggaaatt
     6061 ccctggtaca ttgcccgcac cgaagggctg tggcgctggc tgccgcccct cttggtgatt
     6121 tttcatttct tcgttccgct tttcctgctg ctcatgagag acattaagcg caaccgcaat
     6181 agcttagttt gggtcgcggc cggcgtgctg gtgatgaggc tcttggacac atactggatg
     6241 attgtgccgg gattcgaaca ctccgcgctc cattggctcg acgccagcct gatgatcggt
     6301 atgggcggac tatggctcgg ggtctttctt tggcaattgc aaaagatgcc gctgttgccg
     6361 ctgcatgtag cgctgctcga tgaggaaaca aatgcccatg gctgatacgg aagacgtcaa
     6421 gtatgagacc gaagatttca gcccgcgcgg catccgctgg ttcaccgcgg gattggccgt
     6481 gctggcggta atggctttag tgttgatggg cggtctgctg tggtttctca gcggcggctg
     6541 gcccccgcgc cagggaatcg tcagtcccat ggtgcccgcg cccaatatgt cgccggagcc
     6601 ggatctgcag gtggcatcct cacgtgatta tcaggagatg cacgcggccg aggaagcgca
     6661 attacacagt taccgctggg tgaatcgaga ggccggcatc gccgcgatac cgataacgcg
     6721 cgccatggaa attttggcga gccgcggaat gccggcgcaa aagtcacagc caaagagcac
     6781 gcaaagcaaa caggagcaac cgtgaagtct ggtctttgga gagaattttc ggcggcgatc
     6841 tttgcgctca ccatgagcca catcatggcg ccggaaccgg cgcgctcgca tgaggacaaa
     6901 gtcgctccga ttgcggcgca caatcaaccg gagattttta agaacgtcgg gattgaacaa
     6961 aagctcggca cccagctgcc gttagaaaca acttttcgcg accaaaccgg cacgtcggtt
     7021 tcgctgcaag ggtatttatc ggagaaaccc gccgtgctgg ttttttctta tttcgattgc
     7081 cccatgctct gtcctctcgt gttggagggg ttggtgcgga gcctcaaacc tttgtcgctc
     7141 gatgtcgggc gcgatttcga catcttagtg atcagtatcg acgagcggga tggtcccgag
     7201 acggcgcgaa agaagcaact tgagattgtc aatcggtatg gccgcgatgg cagcgatgtt
     7261 ggctggcact ttctcactgg agaaaagagc gccatcgaaa aagtgaccca aagcgccgga
     7321 ttcacctacg cctgggacga ggccagcaag cag
//
LOCUS       DASUTG010000836         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1610986, whole genome shotgun sequence.
ACCESSION   DASUTG010000836 DASUTG010000000
VERSION     DASUTG010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610986"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF089_19445"
     CDS             complement(<1..360)
                     /locus_tag="VF089_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX6176190.1"
                     /translation="MAAKDVRFSEEARSRVLRGVNVLADTVIVTLGPKGRNVCLEKSW
                     GAPTITKDGVTVAKEIQLEDKFENMGAQMVKEVASKTSDIAGDGTTTATVLARAIFSE
                     GLKLVAAGHDPMSIKRGI"
     gene            complement(376..666)
                     /gene="groES"
                     /locus_tag="VF089_19450"
     CDS             complement(376..666)
                     /gene="groES"
                     /locus_tag="VF089_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6176191.1"
                     /translation="MATKIRPLHDRVIVKRIEEEEKSKGGIIIPDTAKEKPQEGKVVA
                     AGPGRQDDGKVIPLGVKPGDKILFGKYSGTEIKLDGEEHLIMREDDILGVIE"
     gene            complement(773..1153)
                     /locus_tag="VF089_19455"
     CDS             complement(773..1153)
                     /locus_tag="VF089_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX6176192.1"
                     /translation="MMQRISILGLSAIALTLATPFWGRAQSAAEGQKLYMTYCSSCHG
                     DKGRGDGAAGKALPVKPADHTDGNLMNSFTDEFLVTIISKGGAAVGKSSFMPAWGGVL
                     KDNQLQDLLAYLRSIANPSKASGK"
     gene            1389..2009
                     /locus_tag="VF089_19460"
     CDS             1389..2009
                     /locus_tag="VF089_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6176193.1"
                     /translation="MLKIWGRTDSSNVQKVLWCCGEIDLEFNRVDWGGKFGGNKDKAY
                     REMNPNGLVPTIQDGDFVLWESNSIVRYLVEKYARGTLLPSTPEGRAKANRWMDWQLS
                     TFNPIIVPLFFATVRTPADKRDPKAIEAALDKSSQAWQIVDDHLENNKYLAGDEFSIG
                     DIPLGVWAHRWFNLPVERPKQKNLSAWYERLTQRKPYQTHIMIPIT"
     gene            complement(2062..>2951)
                     /locus_tag="VF089_19465"
     CDS             complement(2062..>2951)
                     /locus_tag="VF089_19465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6176194.1"
                     /translation="NKAGLFMAFMFVFAGFGYKIVFVPFHMWSPDVYQGAPTPFTAFL
                     SVASNAAGLAILIRFFYPGISSVTAAGDWAFVPGVEWPQILLFASMITMTVGNLCALN
                     QRNLKRMLAYSGIAHAGYMLMGLAILTNDGLSAILFYIVVYLIMNVGAFLVVAMVANH
                     TGSEDIDGYRGLAWRGAVVPAVCFSIFLFSLTGLPPFAGFIGKFFLFAAVLKEGGASF
                     IILALVAVANSVISLYYYAKVVKVMFLDTPDPADQAVAIAGHNFTLMVPLAVLTIILG
                     VYFSPLSQYTTQSLRFFIK"
BASE COUNT          777 a          777 c          718 g          679 t
ORIGIN      
        1 tatgccgcgc ttgatgctca tcggatcatg gccggcggca acaagcttga ggccctcgct
       61 gaaaatggca cgcgctagaa ccgtagcggt cgtggttccg tcaccagcga tatcggaagt
      121 cttactagcc acttccttta ccatctgggc gcccatgttt tcgaacttgt cttccagctg
      181 aatttctttg gcaactgtga caccgtcctt cgtgatagta ggagcacccc atgacttctc
      241 caggcagacg ttgcggcctt tggggcccaa agtcacgatc actgtatcag ccagtacatt
      301 gacaccgcgc agaaccctac ttcgggcctc ttcgctaaag cgaacatctt ttgcagccat
      361 cttcctattg cctccttatt caattacacc gaggatgtca tcttcgcgca tgatcagatg
      421 ttcctcgcca tcgagcttaa tttccgtgcc agagtatttg ccaaaaagaa tcttatcacc
      481 tggcttcact ccaaggggta taaccttgcc gtcatcctgt cgaccaggac cagcggcgac
      541 gacctttcct tcctgtggct tttccttagc cgtatccgga atgataatac cgcccttgga
      601 tttctcctcc tcctcaatgc gcttcactat caccctatcg tgtaggggac gaatctttgt
      661 tgccatcttt cctcctgaaa tagctgaatt tacgcatccc gcagggacac ggttgattgg
      721 ccaccaattt aatctcgctt agcgaatcgt caagggggtg aaaagaaaat ttctacttac
      781 ccgatgcttt ggaaggattc gcaatgcttc ttaagtaggc aagtaaatcc tgcagctgat
      841 tatctttcaa aacgccaccc caagcaggca taaaagacga cttacccacc gccgccccac
      901 ccttggaaat gattgtcaca aggaattcgt cggtgaacga gttcattaga ttgccatcgg
      961 tatggtccgc aggctttaca gggagcgctt ttcccgccgc cccatcgcct cttcctttat
     1021 ctccatgaca gcttgaacag taggtcatat aaagcttttg cccctctgcc gcactctgag
     1081 cccgacccca aaagggcgta gctagagtga gagcgatagc actcagacct aagatactga
     1141 ttcgctgcat catgacgtgg aggcctatgg aaactaaact tctcttatct agcaggtatg
     1201 taaccgaaac gatagaggtt agtgacaacg tatgacacgt cggtaaagca ggcaatgccg
     1261 catttagtgt cggtaatatc ttcaggtggt gaccgcggca agttttggac ggttactttg
     1321 ccggttgtcg ctatagtcga agtagtgata agaaattaat ctcaatacag cgacaagcgg
     1381 aggtatttat gcttaagatt tggggccgca cagactcatc taatgtgcaa aaggtgctct
     1441 ggtgctgcgg cgagattgat ttagaattca accgggtaga ctggggcgga aagtttggtg
     1501 gtaataaaga caaagcttat cgggaaatga accctaatgg cttagtgccg accattcagg
     1561 acggcgactt tgttctttgg gagtccaact cgattgtgcg ctatctagtg gaaaaatacg
     1621 ctcgcggaac actgctgccg tcgacgccgg aaggccgcgc caaagccaat cgatggatgg
     1681 actggcagct ttccacattc aatccgatca tcgtaccgct ctttttcgct accgtccgga
     1741 ccccggcaga caaacgcgat cctaaagcaa ttgaagctgc cttagacaag agctctcagg
     1801 catggcagat cgttgacgat catttagaga acaataaata tctggcgggc gacgaattct
     1861 ccattgggga tatccccctt ggggtttggg cgcatcgttg gttcaacttg ccggttgaac
     1921 gacccaaaca aaaaaatctc agcgcctggt atgagcgatt aacccagcgc aagccatacc
     1981 aaacccacat catgattccg atcacgtgag ggcccaccga ggtactcagt tctcagtaaa
     2041 cgacgtaacc ggctttggtg tttacttgat gaaaaaacgg agagattgcg tggtgtattg
     2101 gcttagtggc gagaaataga ctcccaagat aatggtcaga accgccaaag gtaccatcaa
     2161 ggtaaagtta tgacccgcga tggcgactgc ttggtccgca ggatcgggag tatccagaaa
     2221 catcacctta acgaccttgg cataataata gagcgagatg acgctgttcg cgaccgcgac
     2281 caacgccaag atgataaagg aggctccgcc ttctttcaga accgccgcaa acagaaaaaa
     2341 tttgccgatg aatccggcga acggcggcaa ccccgttagc gaaaacagga aaatggaaaa
     2401 gcaaactgcc gggacgaccg cgccgcgcca agctagccca cgataaccat caatgtcttc
     2461 actgccggta tgattagcca ccatggcaac cactagaaac gcgccgacat tcataatcaa
     2521 atagacgacg atgtagaaca ggatggcgct aagaccgtca ttcgtcagta tcgccaatcc
     2581 catgagcatg tagccggcat gagcgatgcc cgaatatgct agcatgcgct ttaagtttcg
     2641 ttgattgaga gcacacaggt tgcccacggt catcgtgatc atgctggcga acaacaggat
     2701 ttgaggccat tcaacgcccg gtacaaacgc ccagtcacca gcggctgtga cgctggagat
     2761 gccgggatag aaaaatcgaa ttaagatcgc caggccggcg gcattagagg ccaccgaaag
     2821 aaatgccgtg aacggtgtcg gcgctccctg ataaacatcc ggtgaccaca tatgaaacgg
     2881 gacgaagaca attttatatc caaagccggc aaaaacgaac atgaaggcca tgaacaaacc
     2941 tgccttgttc a
//
LOCUS       DASUTG010000837         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1760909, whole genome shotgun sequence.
ACCESSION   DASUTG010000837 DASUTG010000000
VERSION     DASUTG010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760909"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..822)
                     /locus_tag="VF089_19470"
     CDS             complement(139..822)
                     /locus_tag="VF089_19470"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176195.1"
                     /translation="MSSVYDALIKAGKKAENSGALAVSWQGLNLEWKIMLVVLGLLFI
                     VIVHQLVARVLRAQMEESAVLMTTNLSDAAAGYIASNDALRLKTTVTKYARLSRVAYV
                     FVQDRDGKIIATSLTGLSPELQSAVSSNQELQVSRRKVTVEGKPVHETRAPILDGQLG
                     SAHIGIWAGAVEGDIQKALFKFVWPITLGLLAAVTAVLMLAQPLIRALRRLIELRSAV
                     QPAATQRIK"
     gene            complement(829..2406)
                     /locus_tag="VF089_19475"
     CDS             complement(829..2406)
                     /locus_tag="VF089_19475"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6176196.1"
                     /translation="MYNSYFGFSVSPFRVTPDPSLFYSNPVYEEAYANLRYGIEAKKG
                     FIVITGEVGTGKTTLLRKLMTHTSEGIIHTVYVFNTYLDFIELLQVIHYDLGLGQKEA
                     NKVKLLQELNDFLITQLKDGNIVSVLVDEAQNLSDEALEGLRLLSNLETDQEKLIQIV
                     LMGQPELQARLDQPTLRQLKQRVALQCRLLPLRDQEVCGYIEFRLRAAGYTGKRLFHP
                     DAYRQIAVASKGIPRLVNIICDNALLCAFGRSERIITKDIINEVAGDLRLTPQPELIE
                     TQSISRVIETRPDANGPIGQTATKLPQRKLRRLARFGLETCLVIVGLITVASFIDPRN
                     FLVNAGRTLETAKGNLNEWALFMSHHQAAPAKAIAQVEVETKEQRVIIPQGSSIYKIA
                     TEAYGTKNGLGMDLIKEFNPGIKSLARVSAGRALLLPSLTPETLLRRQADGSYRLIVA
                     SFQSPTAADEYARRLRNKGYQVSITPRKVSEDVLLYRIEIDGLQNPEQADHIWLAGLR
                     NDSFDSGRDSDRNPVSR"
     gene            2635..>3129
                     /locus_tag="VF089_19480"
     CDS             2635..>3129
                     /locus_tag="VF089_19480"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX6176197.1"
                     /translation="MFLKYRFMMVVGLFACVGLTLWANPVLADLDDYIRKPEPNFSWK
                     ENGKSHHKSAGDLIYDLHFISQTWQGNNWQHQLQVYRPQSVAPAAMMLLWVTGGSATP
                     GQIAFGMELARKSKAPVAFLYHIPNQPLLEDQLVEDDLIAETFVRYLKTKDANWPLLF
                     PMVKS"
BASE COUNT          729 a          814 c          807 g          779 t
ORIGIN      
        1 ccctcatggg gcgaagtggt ggccgagctc aacaaggaat tgatttggga tttcgacgga
       61 tacaaaccgg cggattcgtc ctagcatagc aattggcgct gcctttcaga attgagtgta
      121 agatttttgc ggatgagctt actttatccg ctgggttgcg gctggctgta cagctgaacg
      181 caactcgatc agccggcgca acgctcgaat caacggctgt gccaacatca ggactgcggt
      241 cacagccgca agaagtccca acgtaatcgg ccagacaaac ttaaacagcg ccttttgaat
      301 gtccccttca accgcacctg cccagatccc gatgtgcgca ctgccgagct ggccatcgag
      361 tataggcgcg cgggtttcgt gaaccggttt gccttccacg gtgacttttc gccggctgac
      421 ttgaagttcc tgatttgaac taactgctga ttggagctct ggtgacaacc ctgtcaagct
      481 ggtggcgatg atcttcccat cacgatcttg aacgaaaaca tacgcgactc tgctcaagcg
      541 ggcgtacttc gtcactgttg ttttgaggcg gagcgcatca ttgctggcga tgtaccctgc
      601 ggcggcatca cttaagttcg tcgtcatcag gaccgcgctt tcctccattt gagcccgcag
      661 cacgcgcgcc acgagctgat ggacaatgac gataaaaagt agcccaagca ccaccagcat
      721 gatcttccat tcgagattta gaccctgcca agagaccgct agagcacccg agttttcggc
      781 ttttttcccg gcttttatca gtgcgtcata tacgctgctc atatcgctct acctgcttac
      841 cggattgcgg tccgaatcac ggccagaatc aaacgagtca ttccttaggc ccgcgagcca
      901 aatgtgatcg gcttgctcgg gattttgcaa gccgtcgatt tcaatccgat aaagcaagac
      961 atcttccgaa acctttctcg gtgtaattga gacttgatag cctttgttcc tgagacgccg
     1021 cgcgtactca tcagctgctg tcgggctctg aaacgaggcg acaatgagac gataagatcc
     1081 gtccgcctgc ctgcgaagca atgtttctgg ggtcaacgaa ggcaacaaaa gcgcccgccc
     1141 tgccgacacc cgggccaggc tcttgatccc gggattgaac tctttgatca gatccatgcc
     1201 gaggccgttc ttggttccgt aagcttcagt ggcaatttta tagatgcttg agccttgcgg
     1261 aatgatcact cgctgctctt tggtttcaac ttctacctgt gcaatggcct ttgccggcgc
     1321 cgcctgatga tggctcatga acagagccca ttcatttaga ttgcccttag cggtttcgag
     1381 agttctcccg gcgttaacca gaaagttcct tggatcgata aatgatgcca ccgtgatcaa
     1441 gccaacaatg actaagcagg tctcaaggcc gaatcttgcc agacgcctca gtttgcgctg
     1501 aggaagcttg gttgccgttt gcccgatggg cccattcgcg tccggtcgtg tctcgattac
     1561 gcgagaaata ctttgcgttt caatgagctc gggttgcggt gttagcctaa gatcgccggc
     1621 cacttcattg ataatgtctt tggtaatgat tctctcagat cgcccaaagg cgcagagcag
     1681 ggcattatcg cagatgatgt tcaccagccg cggaatgccc tttgaagcga ccgcaatctg
     1741 ccgataagca tcaggatgga atagacgttt accggtatat ccagcggctc tgagtcgaaa
     1801 ttcgatataa ccacatacct cctggtccct cagcggcaga agccggcatt gaagcgcaac
     1861 acgttgtttg agctgccgca gggtaggctg atccagtctt gcctgaagtt cgggctggcc
     1921 catgagcaca atttgtatca gcttttcctg atcggtttcc aagttcgaca gaagtcgcag
     1981 accttcgaga gcttcatcgc ttaagttctg ggcttcgtcc accaggaccg acacgatatt
     2041 gccgtccttg agctgcgtga taagaaaatc gttcaactcc tgaagcagct ttaccttgtt
     2101 tgcttccttc tggcccagac ccagatcgta gtgaatgacc tgcagcagtt cgatgaaatc
     2161 caaatacgta ttaaaaacat aaaccgtatg gatgattcct tccgaggtgt gagtcatcag
     2221 tttacggagc aacgtggtct ttcctgttcc cacttcaccg gtgatgacga tgaaaccttt
     2281 tttagcctca attccatagc gcaaattagc gtaggcttct tcgtaaacag gattgctgta
     2341 aaataaactc ggatcggggg tgacacgaaa aggggagact gaaaatccga aatagctgtt
     2401 gtacatcgta cctagaccgt ccttttgcag tggctgatcg aggtcatagg tttaccgaag
     2461 cagaatcagt ccattgctgg cgtcagcgct ggccaacctt agatcaaagg caaaacaaga
     2521 gtcaagatga aattagatca taaggcgaca cttgtgcgag gagcgggaag cttgcgaccg
     2581 tacacgtgat taaatggatg tcatcgcgcg atgcctacgg ctaaggagta gtaaatgttc
     2641 ctcaagtatc gattcatgat ggtcgttggt ttgtttgctt gtgtcgggtt gaccttgtgg
     2701 gctaatccag tattggcaga tctggatgat tatatccgca aaccggagcc taacttctct
     2761 tggaaggaga acggaaaatc gcaccataag tcggcgggag atctaatcta tgatctccat
     2821 ttcatctccc agacctggca gggcaacaac tggcagcatc agttgcaagt ctaccggccg
     2881 cagtccgtcg caccggcggc gatgatgctg ctatgggtga ccggtggttc ggcaacgccg
     2941 ggacaaattg cgttcggcat ggagctggct cgcaagagta aagcccctgt agcctttctt
     3001 tatcacattc cgaatcagcc gctgttggaa gatcagcttg tcgaggatga tctcatcgcc
     3061 gagacgttcg tgcgctatct caagaccaaa gatgcaaact ggccgctgct ttttccgatg
     3121 gtaaagagt
//
LOCUS       DASUTG010000838         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1464946, whole genome shotgun sequence.
ACCESSION   DASUTG010000838 DASUTG010000000
VERSION     DASUTG010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464946"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF089_19485"
     CDS             <1..826
                     /locus_tag="VF089_19485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6176198.1"
                     /translation="MARQQATGISPMMIRFGKGVGGVRALPKAKGKRPGVIILHERYG
                     IDQHTKDLTVKLAQAGFVGLAPDLFHRFEGDRKAVLRGEQRVDLTDDGALEDLNEAVE
                     YLKRSPNVDATRIGIIGVCQTGRQPLLIAAKRSDIASAVVLYGAIGGKEWVANELRPT
                     PVEDLLAQVSCPVLGIFGEADHIISVEDVTRFRNALEKAKKDYHIRIYRDAPHGWLND
                     TMPGRYRKEPAKDAWNLMMSFLKKCFVKGWDTDRVSWTFESDFSKQYDFKKNVRME"
     gene            835..1494
                     /locus_tag="VF089_19490"
     CDS             835..1494
                     /locus_tag="VF089_19490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6176199.1"
                     /translation="MMRSAESIKTCRESEQREDTRTIRSGVKLPRSFYEQPTVVVAKQ
                     LLGKYLVRNHPQGMTVGRIVETEAYIGPHDLACHASHGRTPRTEVMFGPAGHAYIYFV
                     YGVHYMLNLVTEEFDHPAAVLIRALEPTGGIELMETRRGTDKRRNLCSGPGKLCRAFA
                     IDRSLNAADLCGDIIHIEDRGEPIPKFLARPRIGVDYAGKWKDKPFRFLVRGNEFISR
                     P"
     gene            1565..2035
                     /locus_tag="VF089_19495"
     CDS             1565..2035
                     /locus_tag="VF089_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176200.1"
                     /translation="MARPRYTISINLAPERLLYAGPLIPGAWRHPRDQEEAGSVGGEI
                     LVDTGAFGAMIDVDAVGRLRLPIFGTKEIHGIHGYGSVSCYQAKLVLPAKDPMGEDCK
                     FIKIIECIGVPSLTEKNREHGVKVIGIIGRQFLQDVHLEIDGATGKLKLLVSDR"
     gene            2047..2805
                     /locus_tag="VF089_19500"
     CDS             2047..2805
                     /locus_tag="VF089_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176201.1"
                     /translation="MAERQQDEVEKLIADKLSDAGIKFVTQQAIAGLAPDFIVYAPDG
                     RQFIVEAKNWDFPGLKAEASRQAQHYQDAAKADGAFIVIPSLKRGSPEKGVVTVDGLV
                     PALKAEFENSKTRTHPSEATKVSRIVFAAMPFAPQYEDVFFIAMRQAAEQVRAVCERV
                     DRREFQGSVVDEIQRLIRQSIAVIVDLSEAKPNVLYEAGYAHALNKPCIHICSTSTEK
                     LPFDVNQWKTTMYHPGQTHKLAKELAQRLKAVLD"
BASE COUNT          732 a          772 c          846 g          623 t
ORIGIN      
        1 catggcacga caacaagcaa cgggaatttc accgatgatg atccgcttcg gcaaaggtgt
       61 cggcggagtg cgcgcgctgc cgaaggccaa gggaaaacgt cctggagtga tcattctcca
      121 tgaacgctac ggcatcgatc aacacaccaa agacctgacc gtgaagctcg cccaagccgg
      181 tttcgtcggc ttggcccccg atctctttca tcgcttcgag ggcgaccgaa aggctgttct
      241 tcgcggcgag caaagagtcg acctcacgga cgatggcgct ctggaggact tgaacgaggc
      301 ggtggaatat ctcaagcgct cgcccaatgt cgatgcgacg cgcatcggca tcatcggcgt
      361 atgccagacc ggccggcagc cgctccttat tgctgcgaaa cgcagtgaca tcgccagtgc
      421 ggtggttctc tacggcgcca tcggcggcaa ggaatgggtc gccaatgaac tgagaccgac
      481 accggtggaa gatctgctgg cgcaggttag ttgcccggtg ttggggattt tcggcgaagc
      541 cgatcacatc atctcggtcg aggatgtgac tagatttcgc aatgctctgg agaaagcgaa
      601 gaaggattat catatccgga tttatcgaga tgccccgcac ggttggttaa acgacacgat
      661 gcccggacgc taccgcaaag agccggccaa ggacgcctgg aatctgatga tgtcattctt
      721 gaaaaaatgc ttcgtgaagg gttgggacac agatcgagtt tcgtggactt ttgaaagcga
      781 cttttcaaag cagtacgatt ttaaaaagaa cgtccgaatg gaatgaagca aaagatgatg
      841 cgatcagcgg agagcatcaa aacttgtcgc gaaagcgagc agcgggaaga tacaagaact
      901 atccgttccg gggtgaagct accgcgctca ttttatgaac aaccgacggt cgttgtggcc
      961 aagcagttgc tgggcaagta tctcgtccgc aaccatccgc agggaatgac cgtcggccgg
     1021 atcgttgaaa ccgaagccta catcggaccg cacgatctcg cctgccatgc atcccatggt
     1081 cggacgccgc gcaccgaagt catgtttgga ccggccgggc acgcctacat ttattttgtt
     1141 tacggcgttc actacatgct caacttggtc accgaggagt tcgatcatcc cgcggcagtt
     1201 ttgatccgcg cattggaacc gaccggtgga atagagttga tggagactcg acgagggacc
     1261 gacaaacgcc gcaacttatg tagcgggccg ggcaagctct gccgggcctt tgctatcgat
     1321 cgatcattga acgctgccga cctttgcggc gatatcattc atatcgaaga tagaggcgag
     1381 ccgataccga agtttttggc aagaccccgg atcggggtcg attacgccgg taaatggaaa
     1441 gataaaccgt ttcgtttctt agttcgcggc aatgaattca tttctcgccc gtgatactca
     1501 cacgagcaaa tcacgtccga gttgtttagc tccttaacca gtgaatgagc tagtatcgaa
     1561 gctgatggca cgcccgcgat acacgatttc aatcaacctg gcgccagagc gtttgctgta
     1621 cgccgggccg ctcatccccg gcgcctggag gcatccgcga gatcaagaag aggccggctc
     1681 ggtaggtgga gaaatattag tagacaccgg cgcgtttggc gcgatgatcg atgttgatgc
     1741 ggtcggtcgt ttgcgcctgc caatcttcgg cacgaaagaa atccatggaa ttcacggcta
     1801 cggttccgtt tcttgctatc aagcaaagct tgttcttccg gcaaaggatc cgatgggaga
     1861 agactgtaag ttcataaaaa ttatcgaatg cataggagtg ccttcgctga cagagaagaa
     1921 ccgagagcac ggcgtcaagg ttatcgggat cattggtagg cagtttttac aagatgtcca
     1981 cctggagatc gacggcgcaa ccggcaaact caagctgctc gtcagtgatc gataagtaga
     2041 taatccatgg cagaacgaca acaagacgaa gttgaaaagc tgattgccga taagctcagc
     2101 gacgcgggaa taaaattcgt cacgcagcag gcgatcgcgg ggctcgctcc cgatttcatc
     2161 gtttatgctc cggacggacg gcagtttatc gttgaagcta aaaactggga ttttcccgga
     2221 ctcaaagccg aggcgagccg gcaagcacag cattatcaag atgcggcgaa agccgacggc
     2281 gccttcatag tgattccatc cctcaaacga ggctcgcccg agaagggagt cgttaccgtt
     2341 gacggtctgg tgccggcgct caaagcagag ttcgaaaaca gcaagacacg cacacatcct
     2401 tccgaagcaa ccaaggtgag ccggatcgtt tttgcggcga tgccgtttgc gcctcagtac
     2461 gaggatgtat ttttcatcgc gatgcgtcag gccgcggagc aggtgcgagc agtttgtgaa
     2521 cgagtcgacc ggcgtgagtt tcaaggcagc gtcgtcgatg aaattcaacg gctgattcgt
     2581 caatcgatcg ccgtgatcgt cgatctgtcc gaggccaagc ccaacgtcct ctatgaagcc
     2641 ggttatgctc acgcattgaa caagccgtgc atccatatct gttcaacctc gaccgaaaaa
     2701 ctccctttcg atgtcaatca gtggaagacg acgatgtatc atccgggcca gacgcataaa
     2761 ctggccaaag agctggcgca gcgtttgaaa gcggtgctcg actaagtaga acatggctat
     2821 ggaaacgcga ttgatcctgt ttgccgccgc gccggcaccg gcgttaactt gctggcccgc
     2881 gggaacatgc cggcgaactg acgcgacgaa gttatcctga gacagtatcg gcaaaattcc
     2941 tcgtgacaat ttagttcatg ggcaatcggt aag
//
LOCUS       DASUTG010000839         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_871380, whole genome shotgun sequence.
ACCESSION   DASUTG010000839 DASUTG010000000
VERSION     DASUTG010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871380"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF089_19505"
     CDS             complement(<1..797)
                     /locus_tag="VF089_19505"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6176202.1"
                     /translation="MAAKRNYIIGVDTGGTFTDVVVLADTGEVWTAKASTTPDDFSRG
                     VMDAVGEAALTMGVESSDLLGRTVLFKHGSTVSTNALITRSGVKVGLITTRGFEDTTE
                     IMRAIGRVDGLSDDEIKHVTWITKPEPFVPRERVIGVRERIDYQGNEIVPLNWDDVME
                     AIRRLIEDDKVDAIAVSLMHAWVNPQHENEIRALTAEADPHRKVYWSFGSSLSQVAGE
                     YARANTAILNSYLGPTVERYLKGLEDKLASGGLKGPLLIMQGNGGVA"
     gene            complement(1252..1953)
                     /locus_tag="VF089_19510"
     CDS             complement(1252..1953)
                     /locus_tag="VF089_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176203.1"
                     /translation="MLSVNGIHVYYDAIHALKGVTFSVEAGELVTLLGGNGAGKTTTL
                     KTISGLLRPRRGKIELEGCALDEIDAHEIVRRGVAHVPEGRKVFPRFTVLENLQIGGY
                     SRSIKSLAPDLDFVYRMFPRLHERQKQYAGTLSGGEQQMLAIGRALMAKPKLLLLDEP
                     SMGLAPKIVEQILENIRAINQSGVTVLLVEQNAAMALAISHRGYVLETGEVILSGASS
                     DLAGNDLVRQAYLGG"
     gene            complement(1940..2719)
                     /locus_tag="VF089_19515"
     CDS             complement(1940..2719)
                     /locus_tag="VF089_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176204.1"
                     /translation="MALLELREISKRFGGLQALARVKLDIEAGTIASLIGPNGAGKTT
                     LFNILTGVYLPDGGEIRFLDRPLMGLKPEKITKAGISRTFQNIRLFSHMTVLDNVLVG
                     MHSRMHTSFAEILLRTRNFHDRENGARSRAMELIDLTGLSGRQGEWAHQLSYGEQRRL
                     EIARALAAAPKLLLLDEPTAGMNSAEAQSLVTLFKDLVGRYVDAILLIEHNMRVVMGV
                     SHWVHVLDYGEKIAEGHPDEVRQNPRVIEAYLGAWAEHAQR"
     gene            complement(2720..3967)
                     /locus_tag="VF089_19520"
     CDS             complement(2720..3967)
                     /locus_tag="VF089_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6176205.1"
                     /translation="MSGSLSSLGLLAYIAASSVLAFVFPQSVLAFLLVEASLLLLCYV
                     PAPLKIKIYLAALVLLILIPFLGTFNGYYLEVAIQIGIYVALALGLNIVVGFVGLLNL
                     GFVAFYAIGAYLWAIFGSPQANEFIPGGYFPLSANWFFLFLIFSVAAGALTGTLLGLP
                     VLRLRGDYLAIVTLGFAEVVRAVVNNLDKPINITNGPKGISPIVKPPLFFRPWLEFFG
                     LEIADSTLYPLYFYFLVLLLALLIILITRRLEDSRVGRAWKAIREDELAASAMGVPVV
                     RMKLFAFAVGASFASAVGVLFAAKQVFINPESFTFMESIGVLAMVILGGMGSIPGAIL
                     GATGVTILNLQLLKGLSLWLNGLRQSGAELTLPLLGSYSLSQLPAQFEPAKYERMVFG
                     LILILMMIFRPQGILPPRRDRRS"
     gene            complement(3964..>4791)
                     /locus_tag="VF089_19525"
     CDS             complement(3964..>4791)
                     /locus_tag="VF089_19525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6176206.1"
                     /translation="VLGAFVGVEVLFWLQDAGQLAVLHPAFVLSLVIACGMLISGLGA
                     MALERVAYRPLQGSPRLVALISAIGASFFLQDALRLFESLWRNTFYLNYPTLDVLDQL
                     LPLATNLVVPVKSLMVIATSLLSLGGLYYFVNRTRIGTAIRAVAQDPETASLMGIPVN
                     RVIALTFFVGGAMGGLAGVLFGLHYSLVNPYSGFAPGLKAFTAAVLGGIGNIPGAMLG
                     GMVLGLLEAFAASYLSILTDGAFGAEYKDVFAFLVLIMILIFRPRGLLGETVREGRA"
BASE COUNT         1169 a         1425 c         1296 g          901 t
ORIGIN      
        1 tgcgcgacgc cgccgttacc ttgcatgatc agcagcggtc ctttgagacc gccgctggca
       61 agtttgtctt ccaagccttt caagtagcgc tccaccgtgg gtccgagata gctattcaaa
      121 atcgccgtat tggcgcgcgc atactcgccg gctacctggg acaacgaact accgaaagac
      181 caatagacct tgcggtgcgg gtccgcttcg gcggtcaaag cacgaatttc gttctcgtgc
      241 tgcggattta cccatgcatg catgagactg acggcaatcg catcgacctt gtcgtcttcg
      301 ataagccgtc gaatcgcctc catgacatca tcccagttga ggggaacgat ttcgttgccc
      361 tggtagtcga tccgctcgcg cacgccgatg acccgctccc tgggtacgaa cggttcgggt
      421 ttagtgatcc aggtgacgtg tttgatctcg tcatcggaaa gaccgtcaac ccggccgatc
      481 gcgcgcatga tctcggtggt atcttcgaat ccccgagtcg taatcagtcc caccttcacg
      541 ccgctgcgcg tgatcaacgc attggttgag accgtgctgc cgtgcttaaa cagcaccgtg
      601 cgaccaagaa gatcagaact ctcaacgccc atcgtcagcg cggcctcgcc gaccgcatcc
      661 atcacgccgc gggaaaaatc gtccggcgtg gtcgaagcct ttgccgtcca aacttcgccc
      721 gtgtccgcca agacaacaac atcggtaaac gtgccgccgg tatcgacgcc gataatgtaa
      781 tttctctttg ccgccatgaa attccttccg aagtgaattg cttgcagaaa tatgagccca
      841 agcctggctt gtcaagtgtg agtgaaccct gaattgcgca gttcgaagtg acaaggcaat
      901 tgagcggcga gccctccgga gcaaaggcaa tgggcctctg gttgataacg gtccgcaagc
      961 tccagtgctt tagcggacgc cggaaaaaat tgtcagcgca ggaccaagca ccatacacgg
     1021 cattgaaatt gttcgtttct gtgccttcgc tcctccagcc tcacctctca atcggtacga
     1081 agttgcccat ctactacgga ttcgggttga atgaagcgcg cggcggcgca ggctgctcaa
     1141 aaagttgaca ggcgaggcgc gcgaaaaatc gacaagcgca ggcgtaggta tgcactacgt
     1201 tgaagcgagt cgatcgagcg caacaaagca tgttagggtt ttcagcagcc tctaaccgcc
     1261 gagataggcc tgtcgcacca gatcgttacc ggccaaatcg ctcgacgcgc cgctcaggat
     1321 cacttctcct gtctcgagca cgtaaccgcg atgcgagatc gccaatgcca tggcggcatt
     1381 ttgctcgacc agcagaaccg tgacaccgga ttggttgatc gcgcggatgt tctccaagat
     1441 ctgctcgacg attttcggcg ccagccccat cgatggctca tccagcagca acaatttcgg
     1501 ctttgccatt aacgcccggc cgatcgccag catctgctgc tcccctccgg agagggttcc
     1561 tgcatactgc ttctgccgtt cgtgcaagcg cggaaacatc cgataaacaa aatccagatc
     1621 aggcgcaagc gacttgatgc tgcggctgta tccgccgatt tgcaaatttt ccagcactgt
     1681 gaaacgagga aaaacttttc ggccttccgg cacatgagcc accccgcgcc ggacgatctc
     1741 gtgggcgtca atctcatcca gtgcgcagcc ttccagttcg atcttgccgc ggcgcggccg
     1801 cagcagcccg gagatagttt ttagcgtcgt ggttttgccg gcgccgttgc cgcccagaag
     1861 cgtcacgagc tcgcccgcct cgacggaaaa cgttacgccc ttgagcgcat gaatggcgtc
     1921 gtaatagacg tggatcccgt taacgctgag catgctccgc ccatgcgcca aggtaggcct
     1981 cgatcacccg cggattttgg cgcacttcgt cgggatggcc ttcagcgatc ttttcaccgt
     2041 agtcgagaac atggacccaa tgcgacacgc ccataacaac gcgcatattg tgctcgatca
     2101 gcagtatcgc atcgacatat ctgccgacca aatctttgaa caaggtaacc agcgactgtg
     2161 cttcggcgga attcattcct gcggtcggct catcgagcag cagcagcttc ggcgctgcag
     2221 ccaaggcacg cgcaatctcg agccggcgtt gttctccata ggagagctgg tgcgcccact
     2281 caccttggcg gccgctgagt ccggtcaaat cgatcagctc catggcccgg ctgcgcgctc
     2341 cgttctcgcg atcgtggaag ttccgggtgc gcagcagaat ctcagcgaaa ctcgtgtgca
     2401 tgcgtgaatg catcccgact aagacgttgt ccagaaccgt catatgcgag aacaggcgta
     2461 tgttttgaaa cgtccggctg atgcccgctt tggtgatttt ttccggcttt aaacccatta
     2521 agggccggtc cagaaaacgg atttcgccgc cgtcgggcag gtagactccg gtaaggatgt
     2581 tgaacagcgt ggtcttgccc gcgccgttcg ggccgatgag cgaggcgatt gtgccggctt
     2641 cgatgtcgag cttgacccgc gccaaagcct gtaatccacc gaaccgcttg gaaatctctc
     2701 gcagctccag cagcgccatt taacttcgcc ggtctcgccg cggcggcaga ataccttgcg
     2761 ggcggaagat catcatcaga atcaaaatta aaccaaaaac cattcgttcg tattttgccg
     2821 gctcgaactg ggccggcaac tggctcaacg aatagcttcc caaaagcggc aacgtcaatt
     2881 cagcgcctga ctggcgcagg ccgttgagcc atagcgataa ccccttgagc aattggaggt
     2941 tgagaatcgt cacaccggta gcgcctagaa tggcgccagg aattgacccc atgccgccga
     3001 gaatcaccat agctagaact ccgatcgatt ccatgaaggt aaaactctcg ggattgatga
     3061 aaacctgttt ggccgcaaac aacactccca ccgcggaagc gaaggacgca cccaccgcga
     3121 acgcaaacag cttcatgcgc actaccggaa cacccatcgc cgatgccgcc aattcgtctt
     3181 cgcggatcgc cttccaggcc cgcccgacgc gcgagtcctc gagacggcgt gtgatgagaa
     3241 tgatgagcag cgccaacagt agcacaagaa aataaaaata cagcgggtac aacgtcgagt
     3301 cggcaatttc caatccgaag aactccagcc acggtctgaa aaacagcggc ggtttaacga
     3361 tcggcgagat gcctttcgga ccattggtaa tgttgatcgg tttatcgagg ttgttgacga
     3421 ccgcgcgcac cacctcggca aatcccaggg tgacgatggc cagataatcg ccgcgcaacc
     3481 gcagcaccgg cagccccagc aacgttcccg tcagggcacc cgcggcgaca ctgaaaatga
     3541 gaaagaggaa aaaccaattg gccgacaatg gaaagtagcc gccgggaatg aactcattgg
     3601 cttggggcga accaaagatc gcccacaggt aggcgccgat agcataaaag gcaacgaagc
     3661 cgagattgag caggccgaca aagccaacga cgatatttaa ccccagagcc agcgcgacat
     3721 agatgccgat ttgaatggca acttccagat aatagccgtt aaatgtaccc aaaaacggga
     3781 tcaggattag cagcaccaac gcagccaagt atatttttat cttgagcggt gccgggacat
     3841 agcagagcag cagcaatgaa gcttcgacca ggagaaaggc caagacagat tggggaaaga
     3901 caaaggccag cacggagctt gcagcgatgt atgctaacaa gcccaaggac gatagcgagc
     3961 cgctcacgcc cgtccctcgc gcacggtctc accaagcaac cctctgggac gaaaaatcaa
     4021 gatcatgatc agcacaagaa aggcgaagac atccttatat tcagcgccga aggcgccgtc
     4081 ggtaagaatc gataagtacg aagcagcgaa tgcctctaac aagccgagca ccatgccgcc
     4141 tagcattgcg ccgggaatat tgccgatgcc gcccaggacg gcggcagtga aagctttcag
     4201 ccccggcgca aacccgctgt agggattgac caggctgtag tgaaggccaa ataacacccc
     4261 ggctaaacct cccatcgcgc cgccgacgaa aaatgtcagc gcaatcaccc gattgaccgg
     4321 tatccccatc agcgacgcgg tttccggatc ctgcgcgacc gcgcgaatcg ccgtgccaat
     4381 gcgcgtccga ttaacgaagt aatagaggcc gcctaagcta agcagtgacg tggcaatgac
     4441 catcagggac ttcaccggaa cgacaagatt agttgccagc ggcaaaagct gatcgagcac
     4501 atccagggtc ggataattga ggtaaaaggt gttgcgccag aggctttcga acaaacgcag
     4561 cgcatcttgc agaaaaaatg acgcgccgat cgcggagatc agcgcgacca gacggggact
     4621 accctgaagc ggacgatagg ccacccgctc cagggccatg gcgcccaggc cgctgatcaa
     4681 catgccgcaa gcgatcacca gtgacaagac gaaggccggg tgtaagaccg ccaattgtcc
     4741 agcatcctgc agccaaaaga gaacttcgac accgacaaac gcgccgagaa c
//
LOCUS       DASUTG010000840         8386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1611580, whole genome shotgun sequence.
ACCESSION   DASUTG010000840 DASUTG010000000
VERSION     DASUTG010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611580"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF089_19530"
     CDS             <1..399
                     /locus_tag="VF089_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176207.1"
                     /translation="WAKGLESGFVRPLIQTTLKPHPDLPNVAVAIQHAKSKEDQALLR
                     LAAQAYGPASIAYSVPPGMVKDRLETLQRAFMDTMKDAEFLAEAKKSNLVVSPLDGPT
                     IEQTVAELYRAEPKLLERFRNVIEVPQSQR"
     gene            complement(1027..2712)
                     /locus_tag="VF089_19535"
     CDS             complement(1027..2712)
                     /locus_tag="VF089_19535"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6176208.1"
                     /translation="MKNHFDGGSALVEAFRKLGVEYILSSPGTEWAPVWEAMADQIHR
                     KKEGPKLLDVWHETLAVDMAAGYTLATGKMQAVLLHAGAGLLQGAMGIHGAFVTGVPM
                     VVISGESMTYGEQPEFDPGAQWINNLSIVGGTTGLVDTIVKYASVAGSAYTLYESVIR
                     AGELAQRQPTGPVYLSVSTETLMDAWTPPAHARTIPRAPRLLSAPEDIEKLAAQISKA
                     KHPVVLTEGAGQDVETYEALVALCEKFALPIVEKPGALFANFPKDHPLYQGTDIKPFW
                     DDMDLAIVVRTRAPWYPPSNRPPKALIAMVDEAPHNSAMVYQSHQADMYLEGNAAQTL
                     RDLANAARVDKAAVEARRKDLAAAHDALVEKRNAAQAAARKKTPIDPVWLCASLNAVL
                     PKDTIYIDEVTTHTATLRQHLVWNDPQCLFTRQGGLGQGLGLSLGIKLGKPERQVVTL
                     IGDGAFLYNPALGSLGAARDYKLPTMTVIFNNKKYAAMQGMHQKMYPDGIAAEIDLYH
                     GTHINPPDFVKVAESVGGYGEQVTDPEKLQDALKRGLEANQSGKPAIIDVIVT"
     gene            complement(3276..4070)
                     /locus_tag="VF089_19540"
     CDS             complement(3276..4070)
                     /locus_tag="VF089_19540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176209.1"
                     /translation="MTSAAQFAGHADLPGVRLWYTDSGGDGVPVVLLHANTGNADGFQ
                     HNIPSFVEAGYRAIAFDRRGWGRSTATPDTGPQPGTIAEDLHALMEHLSIDRFHLVGV
                     AGGGFAAYDYVLWHPERLRSLVVAASGGAIVDEELSKLREKTTLPGFRSWPPEFREVS
                     MGYMATNPEGLKRWIEIHNNSQQKGAPVQPQRTTITFEKLATIRVPTLLMPGDQDIQC
                     PPWVMRRQLAHIPHAEFIVLSEASHSINWEQPETFNRHVLEFIRKY"
     gene            complement(4348..5532)
                     /gene="solA"
                     /locus_tag="VF089_19545"
     CDS             complement(4348..5532)
                     /gene="solA"
                     /locus_tag="VF089_19545"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEX6176210.1"
                     /translation="MATNFDTIVVGLGATGSAAVYQLAKRGSKVLGVDRFSPPHANGS
                     SWGESRIIRRAIGEGEEYVPMAMRSYELWREIEQETDREFLMITGGLTLESRSSQGVM
                     HGRRDFLDQAIRCAEKFNIRHDILEAADIRKRYPQFVVTDERAYFEYETGFLRPELCL
                     KAQLELGRKHGAVVQTDEIVTSIESDGPSGVTVKTSRGAYSAEKVIIAAGPWITRFLP
                     PSYAHLFTIYRQVMYWFDIREDCRSEFAPPGFPIFIWIFDNCGEFGFYGFPTLEGKTI
                     KVATEQFTQSTDPDHVQRLVRTEEERAMYKRYIEGRLPGISSRCAIAASCLYTLTPDS
                     NFVIDVHPDDDRIIIASPCSGHGFKHSAAIGEALAEQVIDGKSKIEMSSFSLKRFKKT
                     IQ"
     gene            complement(5779..7041)
                     /locus_tag="VF089_19550"
     CDS             complement(5779..7041)
                     /locus_tag="VF089_19550"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6176211.1"
                     /translation="MNEAQSFAPLLIVVMLAFLVPLLLSSVRGRLAVPIVVGEIVAGM
                     IVGGSGFGWVVPDDGALNLLKELGFVLLMFLSGMEIDFRSLRRSDPRQAKQQMVWRPL
                     PLAVLNFGITLLLAGVFAWGLKLIGATTDPWMMGLILSTTALGVVVPILKEAGLNQTL
                     FGQTILLATVIADFVTMFLISILVAVISSGLTAEILLVFLLFVAFFFAARFGSFLSRI
                     EPLQRIIEELSHATAQIKVRAAFSTLLIFVVLAEKLGIEIIVGAFLAGAIVALLRTPE
                     DRELSSQLEGIGFGFFIPIFFIMVGVDFNLPALLSSTGALLSVPLLLGAAIAVKIIPA
                     MVFRLNHTWRESLAAGILMCPRLSLIIAAAAIGEKLGIIDESVNAAIVLVAIVTVTAS
                     PLAFLRVFPAPRVKVARAPVTDTGLAAP"
     gene            7381..>8386
                     /locus_tag="VF089_19555"
     CDS             7381..>8386
                     /locus_tag="VF089_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176212.1"
                     /translation="MDHAPESKWQEPTDNKTRAGLGKFLRPNTPYDNYMEAQGIPIYR
                     DIGVRRVQDLPRKPWKRMGGSGTFIQLLGTEGLWGCFVVEVPGAGALNPQKHLYEEQF
                     LVVEGRGTTEVWAEGNGKKLTFEWQKGSLFAIPMNANHRIINATSSPALLLAGNTAPN
                     IMNNFDNEDFIFNCPYQFKDRFDPSDDYYKYKEDITPDPVRGLAMRQTNIIPDVISCE
                     LPLDNRRSPGYRRIEPHMVGQQFYYWIGQHETGRYSKAHAHGSAAVLICLSGKGYTYT
                     WPATLGTKPWEAGKTELIRRQDYEPVGMVSAAPMSGNWFHAHFGASKEPLRLTAWFGP
                     N"
BASE COUNT         1807 a         2421 c         2348 g         1810 t
ORIGIN      
        1 tgggcgaaag gactcgaatc cggattcgtt cgaccgttga ttcagacgac gctcaagccc
       61 catccggatc taccgaatgt tgcagttgca attcagcacg ccaagagcaa agaggatcaa
      121 gcgctgctgc ggcttgcggc acaagcgtac ggccccgcct cgatcgctta ttctgtgccg
      181 ccgggcatgg tcaaggatcg tttggaaacc ttgcagagag cttttatgga tacgatgaaa
      241 gatgcggagt tcttggcgga agcgaaaaag tcgaacctgg tagtcagtcc gctcgatgga
      301 ccgacgatcg agcagacggt ggcagaactt taccgcgccg aacccaagct gctcgagcgc
      361 ttccgcaatg ttattgaggt cccacagtcg cagcgctaag cgtcacccca gctcgtaaat
      421 ctatgaatct gtgccggcat ctcttgcccc ctttatctct tccacaggca atcgatcgag
      481 gtgctgctcg cacgaatcga gcgagtctcc aactgcatgc cctgacatta gcagaactca
      541 tatggagtgc gttctcttca tcctcctgag ttcgagtaat cagcaatagg atccgtgcct
      601 tcagggattc agattaatct tcgggagaaa ttaaacgtca ggattttctt ctagcctttg
      661 tgctttcctt cgttcatccc gcacaagcac cttagattca actttacact tcttgctcgc
      721 gatcgctccg tctcgcacag ggagcgcagc atggcccggg cgattcgagt atggacacag
      781 cgctgatatc acaggcgccg atcgtcaggt attttcacct atgtagctcg tgattctgta
      841 cggcatgtgg tcgccagctg ctcggcgaac tgctgatggc tacgacgttc ggggaacacc
      901 tggctcgcga aaattccgcg ttgttcggtt ctcggttgaa attgaggcgc gaagagggcg
      961 gttgctagac ttatttgagt ataaatttgc gaaacacgcg tcggaacaac cgcctctccg
     1021 tctgatctat gtaacgatca cgtcaatgat cgcgggcttg ccgctctgat tggcttcgag
     1081 gccgcgcttc agggcatcct gaagcttctc cggatccgtc acctgctcgc catagccgcc
     1141 gacggattcg gcgaccttga cgaagtcggg cggattgatg tgggtgccgt ggtagaggtc
     1201 gatttccgcc gcgatcccgt ccgggtacat cttctggtgc atgccctgca tagcggcgta
     1261 tttcttgtta ttgaagataa cagtcatggt cggcagcttg tagtcgcgtg ccgcgccgag
     1321 gctgccgagc gccgggttgt ataggaacgc gccgtcgccg atcaaggtaa cgacctgccg
     1381 ttccggcttt cccagcttga tccccaacga aagacccaat ccctgtccga ggccgccctg
     1441 gcgggtgaac aggcactgcg ggtcgttcca gacgagatgt tggcgcaagg tggctgtgtg
     1501 ggtggtgacc tcgtcgatat agatcgtgtc tttgggcagc acggcgttga gcgatgcaca
     1561 cagccacacc gggtcgatgg gtgtcttctt gcgcgccgca gcctgggccg cattcctttt
     1621 ctcgactagc gcatcatgcg ccgcggcgag atccttgcgt cttgcctcca ctgcggcttt
     1681 gtcaaccctc gcggcattgg ctaggtcgcg cagcgtctgc gccgcattgc cttccaggta
     1741 catgtcggcc tgatgactct ggtaaaccat ggccgagtta tgcggcgcct catccaccat
     1801 ggcgatgagg gctttgggcg ggcggttgct cggcggatac cacggcgcgc gggtgcggac
     1861 gacgatcgca aggtccatgt cgtcccaaaa tggtttgata tcggttcctt gataaagcgg
     1921 atggtccttg gggaagttgg cgaacaaagc gccgggcttc tcgacgattg gcagagcaaa
     1981 tttctcacac agtgctacca gagcctcata ggtttcgaca tcctgaccgg cgccctcggt
     2041 gagcacgacc gggtgcttcg ccttggagat ctgcgcggcc agcttttcga tgtcttccgg
     2101 cgcgctcagc aggcgcggcg cgcgaggaat cgtgcgggca tgggccgggg gtgtccatgc
     2161 gtccatcagc gtttctgtgg aaacggacag gtaaaccggt ccggtcggtt gccgctgcgc
     2221 cagctcgccg gcgcggataa cgctttcata caacgtatag gcgctgcccg cgaccgaggc
     2281 gtacttgacg atcgtatcga ccagccccgt cgtgccgccg acgatgctga gattgttgat
     2341 ccactgtgcg cccggatcga actccggctg ctcaccatac gtcattgact cccctgaaat
     2401 caccaccatc ggaactccgg tgacaaaggc gccgtggatg cccatagcgc cctgcagcag
     2461 gccggcgccc gcgtgcagca atacggcttg cattttgccg gtggccagcg tgtatcccgc
     2521 tgccatgtcg acggcgagcg tttcgtgcca tacgtccagg agcttgggac cctctttctt
     2581 ccggtgtatc tgatcggcca tggcttccca gaccggcgcc cattcggtgc ccggcgagga
     2641 caggatgtac tcgactccga gcttgcggaa tgcttcgact aaggcgctgc cgccatcgaa
     2701 atgatttttc atttttgatc tcccctaacg gttttcttgt ggtgtgtatt atctcacgtg
     2761 tgaatgcgca agagcccatg gcgagctgcc gataataaat ctggagcagc gacgtcgggc
     2821 cctctagcgg gtcgaatctt tagaggtttt tgaaaaaggc tcatctgctt cgtctcaatg
     2881 ctcgcttcga cgtcgctaca gatacgccct ccactgtcga cttctcgcgc gtctcgtacc
     2941 tgagaccgtt ttgaacagcg gtttagcttt gcatcgctag ctgctggcgc taagcgatgg
     3001 ctcagacttc tgcttcttag cgactgcgcg gggctgcgcc actcacctag gtcttctggc
     3061 gattatcgtg ctgtacaact cgggccttct attcaaggcg ttcaaaccgg ggcaaccgct
     3121 gcgctcggct aagttaagtc gttcgacaga atcaactaga aatcgctctc tcaactagaa
     3181 aactctgtaa gataagtttc acacttgcac taactgtgcg acttcttaac ggccttgacg
     3241 cagatcagaa ataaaaactt tgttcgggga atccgctaat acttgcggat aaactcaagc
     3301 acgtgccggt taaaggtttc gggctgctcc cagttgatcg agtgagaagc ttcggagaga
     3361 acgatgaact cggcgtgtgg aatatgggcg agctggcggc gcatgaccca aggcgggcac
     3421 tggatgtcct gatcgcccgg catgagtagt gtcggcacgc gaatcgttgc gagcttctcg
     3481 aacgtgatcg tcgtgcgttg cggttgcacg ggcgcgcctt tctgctgcga attgttgtgg
     3541 atctcgatcc agcgcttcag accctccgga ttggtggcca tgtagcccat gctgacttcg
     3601 cgaaactccg gcggccaact cctgaaaccc ggaagcgtgg tcttctcgcg cagcttgctc
     3661 aactcttcat cgacgatggc gccgccgctg gcggcaacca cgaggctgcg gagccgctct
     3721 ggatgccaga gaacgtaatc gtaggcggca aatccaccac ccgcaacgcc gacgagatga
     3781 aagcgatcga tactcaggtg ttccatcaac gcgtgaagat cttccgcgat ggtgccgggc
     3841 tgcggcccgg tgtccggggt tgcggtgctg cggccccatc cgcgccgatc gaaagcgatg
     3901 gcgcgatatc ccgcctcgac gaaggacgga atgttgtgct gaaaaccatc ggcgttgccg
     3961 gtgttggcgt gaagcagcac gacgggaaca ccgtcgccgc cgctgtccgt gtaccacagg
     4021 cgcacgccgg ggagatcggc atgtccggca aattgtgcag cagatgtcat gatcaaacgt
     4081 tcaatagtag gtgcaaccgg cggtcgcccc tacactcgtt cgtgattact attgatgcgc
     4141 tctagctgtc aaaccgaact tgccaatgga aattcctagt ctgccctccg tatagcgcct
     4201 ttgccgccgc gggcacgatg ctcggttagg actgtctcct tctggaagtg agatcaagtt
     4261 cgctcttgtt ttggcctggg tttcgctgag cacgtcggaa tgcgcggaac cagcgaatat
     4321 ttctatatcg tgataaggat gaggcgttta ttgtatcgtt tttttgaatc tcttcaggct
     4381 gaaactgctc atctcgatct tgctttttcc atcgatcacc tgctccgcca acgcctcgcc
     4441 gatcgctgcc gagtgtttaa atccatggcc cgaacagggc gatgcaatga taatgcgatc
     4501 gtcatccgga tgaacatcga tgacgaagtt cgaatcgggc gtcaacgtat acaggcaact
     4561 tgctgcgatg gcacatcgcg aagagattcc cggcaagcga ccctcgatat atcttttgta
     4621 catggctcgc tcttcttccg ttctgaccag tcgctgcacg tgatctgggt ccgtgctctg
     4681 cgtgaactgc tcagtcgcaa ctttgattgt tttgccctcg agcgtcggaa agccatagaa
     4741 gccgaactca ccgcagttat caaagatcca gataaaaatc ggaaacccgg gcggcgcaaa
     4801 ctccgagcgg caatcttccc tgatatcaaa ccagtacatg acttgccggt aaatcgtgaa
     4861 cagatgcgcg tacgacggcg gcagaaaacg ggtgatccag gggccggcgg cgatgatgac
     4921 cttctccgcg gagtaggcgc cgcggctggt cttgaccgtc acaccggaag ggccgtcact
     4981 ttcgattgag gtaacgattt catctgtttg aacgactgcg ccgtgcttgc gccccagttc
     5041 gagctgcgcc ttgagacaaa gttcgggtct tagaaatccc gtttcgtatt cgaagtaagc
     5101 gcgctcgtcg gtaacgacaa attgcggata acgttttctg atgtctgcgg cctcgaggat
     5161 gtcgtggcgg atgttgaact tctccgcaca gcggatcgcc tggtctaaga aatcacgccg
     5221 gccatgcatg acaccttggc tacttcggct ctcgagagtc aggccacccg tgatcatcag
     5281 gaactccctg tccgtctctt gttcgatctc gcgccacaac tcataggagc gcatagccat
     5341 gggaacgtat tcctcgcctt cgccaatcgc ccggcggatg attcgactct caccccatga
     5401 cgagccgtta gcatgcggcg gggaaaaccg atcgacgccg agcactttgc tgcctcgttt
     5461 cgccagttgg taaaccgcag cgcttccggt ggcaccgagg cccactacaa tcgtgtcgaa
     5521 gttagtggcc atgactctcc tggtggacgg tcatccgctt gacggactgg gctcgttgac
     5581 atcggctgca tgcgggacgg actcaggcgc tcctgccatg gttgcgcccc gccttgcgta
     5641 tttaattgag acaaggagaa gcatattcat ctggcttgct aaatcagtgg gaagacaaag
     5701 cagatatcaa atggacgtgg aagaacctac gggaacaata ttggttgtag ccaaatgatc
     5761 agctctctgc ccgagccgct aaggagcggc aaggcccgta tctgtcaccg gagcgcgcgc
     5821 tactttcacg cgcggcgcgg ggaaaacccg taggaacgcc agcggcgatg ccgtaactgt
     5881 gacaatcgcc accaaaacga tagccgcgtt caccgattca tcgataatcc ccaacttctc
     5941 gccaatcgca gcggcggcga tgatcaacga gaggcgcggg cacatcagta tgccggcggc
     6001 cagcgactcg cgccacgtgt gattgagacg aaacaccatt gccgggataa tttttaccgc
     6061 aatcgcggca ccgaggagca gaggaacgga gagaagcgct ccggtcgagg acagcagcgc
     6121 cggcagattg aaatcgacgc cgaccatgat gaagaaaatc gggatgaaaa atccaaagcc
     6181 gataccttcg agctggctgg acagctctct atcctcgggg gtgcgcagta gcgcgactat
     6241 ggcgcccgcc aaaaaggcgc cgacaatgat ctcgatgccg agcttttcgg cgagcacgac
     6301 gaaaatcaat agagtggaaa aagccgcgcg aaccttgatt tgcgccgtcg catggctgag
     6361 ctcctcgatg atcctttgca gtggctcgat gcggcttagg aacgagccga atcttgccgc
     6421 gaagaaaaac gccacgaaca atagaaagac cagcagaatt tcggcagtca agccgctgga
     6481 gatgaccgcg accaggatgc tgatcaaaaa catggtgacg aaatcggcga tgaccgtagc
     6541 cagtaaaatc gtctgtccaa acagagtctg gttcagaccg gcttccttga ggatcggcac
     6601 cacaactccc aacgcggtgg ttgaaagaat caatcccatc atccatggat cggtggtggc
     6661 accaatcaac ttcaggcccc aagcaaacac gcccgccagt agcagggtga ttccgaagtt
     6721 aaggacggca agcggcagtg ggcgccacac catctgctgc tttgcctgac gggggtcgct
     6781 gcggcgcaag ctgcgaaaat cgatctccat gcctgagaga aacatcagca gaacgaatcc
     6841 caactccttc aagagattga gcgcgccgtc gtcgggtaca acccaaccga aaccgctgcc
     6901 accgacgatc atccccgcga ctatttcgcc gacaacgatg ggcaccgcta ggcgcccgcg
     6961 cacggacgag agcagcagcg gcaccaggaa cgccagcatg acaacaatca gcagcggcgc
     7021 aaacgattgc gcttcgttca tgaaaaagct taaacctttc gcaaagggaa ctcagtccgg
     7081 caactattat aattattttt tggatgtgca agatcaccag cgacaggccc gcactcctta
     7141 tcacgatccc aggtgcgtcc tttgcctcaa ctcgtctcag atctctaagg agctttgttt
     7201 tcatcaggag cacgtctcga ccacgaatac gtctgtttct cctttcattt tttcctcctt
     7261 gccccgaatc gctctccgtt tgactccgat ttccgcagct tataagttat tttaatccgc
     7321 tgaactggct gtctatagta ccagtcgcag gcccaacaag cagaatcaca aggaggaatt
     7381 atggatcacg cacccgagtc aaagtggcag gagcccaccg ataacaaaac cagagccggt
     7441 ctcggcaagt ttctccgccc gaatactccc tatgacaatt atatggaagc tcagggcatt
     7501 cccatttacc gcgatatcgg cgtccggcgg gtccaggacc taccgcgcaa gccatggaag
     7561 cgcatgggcg gcagcggcac cttcatccag ttactcggca ccgagggatt gtggggctgc
     7621 tttgtcgtcg aagtcccagg cgcgggagcg ctcaatccgc agaagcatct ctacgaagag
     7681 cagttcctgg tggtcgaagg ccgcggcacg accgaagtct gggctgaggg caacggcaag
     7741 aaactgacat tcgaatggca gaaaggatcg ctatttgcga ttccgatgaa cgccaatcac
     7801 cggatcatca acgccacttc gagtcccgca ttgcttctgg ccggcaacac ggcgcccaac
     7861 atcatgaaca acttcgataa cgaagatttt attttcaact gcccttatca gttcaaagat
     7921 cggttcgacc caagcgacga ctattacaaa tacaaagaag acatcactcc cgatccggtg
     7981 cgcggtctgg cgatgcgtca aaccaacatc atacccgacg tgatttcctg cgagctgccc
     8041 ctcgacaatc gccgctcgcc gggctaccgg cgcatcgagc cccatatggt gggacaacag
     8101 ttctattatt ggatcggaca gcacgaaacc ggccgctact cgaaagcgca tgctcacggt
     8161 tcggcggcgg tgctgatttg cctgagcggc aaaggctaca cttacacctg gccggcgacg
     8221 ctggggacca agccctggga ggccggcaaa acggaattga tcaggcgaca ggattacgag
     8281 ccggtcggca tggtcagcgc cgcgcccatg agcggcaatt ggttccacgc ccacttcggc
     8341 gccagcaaag agccgctgcg cctcaccgcc tggttcggcc ccaatg
//
LOCUS       DASUTG010000841         6062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_575006, whole genome shotgun sequence.
ACCESSION   DASUTG010000841 DASUTG010000000
VERSION     DASUTG010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575006"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF089_19560"
     CDS             complement(<1..416)
                     /locus_tag="VF089_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176213.1"
                     /translation="MRNGIKAIDFMYYVATPEFIAKWNEAKKGELICRMEKAIGGLPQ
                     YDSIEAMIAKMDEAGVDKVFITQCKMWSYRNKWMYMDTKLEEVAQYTTRYPSRFVGLA
                     SYNPFRIKESLLELEQAIKVYRFKGVYIHIYGFDIPL"
     gene            complement(501..1262)
                     /locus_tag="VF089_19565"
     CDS             complement(501..1262)
                     /locus_tag="VF089_19565"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6176214.1"
                     /translation="MKLQGKTALIAGGGGGIGRAVALALAREGAKTAVADIVKDNAEK
                     VSTEVKALGVDAIACQVDLTKKVDVDRMVNEVLARFGQIDILVNCQGWDRLEPFVESQ
                     EETWEKLLAINFKSVLYTAKAVLPQMISHGGGKIVNISSDAGRVGSSWEAVYAGAKGA
                     VIAFSKTIAREVARYKINVNVVCPGLTETPLLQAVRSQSEQTAKIIEAVTKATPFRRP
                     AKPEEIAEAVLFFTSPAAEFITGQTLSVSGGLTMV"
     gene            complement(1259..2095)
                     /locus_tag="VF089_19570"
     CDS             complement(1259..2095)
                     /locus_tag="VF089_19570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX6176215.1"
                     /translation="MTFVAGIDIGAKSTKAVIMDGEKRICGKASVKTRPDFAAIAKEA
                     LDGALQRAGRKETDLNYIATTGFGRYNVPFRDVQITDITCVGRGAVFLFPKTRCVLDI
                     GAQSTRALRVSDTGKVREFRTNDKCAAGAGGFIERACKYLEVNIDQVGELSIQANKPQ
                     TISSVCAVLAESEIINHVSNGETIENILRGVHNSLASRALALLKRSGMEDEVTFVGGV
                     ARQKGMVKALEETLKRKINVSEEPELVGALGAALLALRRLEKIREDKKTAVLGEEARA
                     AS"
     gene            complement(2130..2963)
                     /locus_tag="VF089_19575"
     CDS             complement(2130..2963)
                     /locus_tag="VF089_19575"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX6176216.1"
                     /translation="MIYAAGIDVGSTQTKGIILNDKLEIVARALTDTGAYVTRAAERC
                     FVEALRISQVKREDVAYVVGTGYGRYKVTFGDAQITEISCHARGASYLFPSTRTVIDM
                     GGQDAKGIKIGIDGDVKDFVMNDKCAAGTGRFLASAAEALGMTLGDIGDISLQAKNPV
                     RLTTVCTVFVESDIMSYLAQGKKTEDILGGVHSAIAARTISLVRRVGIEPEVTFTGGV
                     SLNIGMVRALEEKLGMKLNVSPDSHFVGALGASIFALERATKATHETQQGTAIQAGPT
                     A"
     gene            complement(2960..4261)
                     /locus_tag="VF089_19580"
     CDS             complement(2960..4261)
                     /locus_tag="VF089_19580"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX6176217.1"
                     /translation="MAATAVAEKTQYQGEMHLLQKELMGRYFERVTRAAEQGEGKAVY
                     MLISGNPVELIQAFDLIPIYPEIDALQLAAKKSALPFIQKAEELGYSMDNCAYVKADV
                     GMFLGDRKTPFATIPLPSLILCNYVGCNVYLQWFEHLAEYCDGQLYNLDIPFLRDPSG
                     HPSPEDISYVVRQLEEVIEICERLTGKKLDYARLKQIVSRSSETEEMWSRIKGLTKNI
                     PSPFDAYFDSVTMMAPLYCLRGTEEGVRFFETALKEMEEKVRMGAGPLPEERFRFIIE
                     GPPPWPYLRTFRDMFSKWGAVAVASTYSTVGGLWEFGFRHDSNHPLESIADHMLQWNV
                     TNRNFLQRYQQIRRYVEEWHADALVIHSVKSCRLFSAGQGDMREYFTKDLNIPTLLIE
                     SDLEDPRYFSEAQLKNRIDAFFESLEHKKMVRAGAEAGGAA"
     gene            complement(4271..5428)
                     /locus_tag="VF089_19585"
     CDS             complement(4271..5428)
                     /locus_tag="VF089_19585"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX6176218.1"
                     /translation="MTTVENLVSRYRDPLETYPLELASDWKKMHPDKKLVGCYPVYTP
                     FELIHAAGMLPVGVIGAGNRLEIAHADSRFQSFICSIVKSTLELGLIDKLKFLDGIVF
                     HSICDPARNLASVFTRNFPDLMVEYIHFPQNMTSPHTIDYLMAEYKRIGETYEKLSGK
                     KITDDGLSQSIALYNRQRRMIRDLYAIRRETPQNLSTVECYVLTRIGTLLPPEEHIAI
                     LEEAIPQLRRRNEKPKDRIRVVLEGSFCEQPPIELLDGLESAGCYILDDDFLIGWRWF
                     LGDIPTDGDPLRNLANAYLNQSVYSGVKHDTREPKSKHLIDKFRHTGADAVLILAAKF
                     CEPALFDYALYKRALEKEGIPHLSLEFEEKMWIFDKARTEVETFVESMLFD"
     gene            5675..>6062
                     /locus_tag="VF089_19590"
     CDS             5675..>6062
                     /locus_tag="VF089_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176219.1"
                     /translation="MARAVRSGSIAIIAGQRAEDATWGIPESPEFSSVQRLMNEFQSH
                     AAHEERWLSSYQEIAKETIDPQIRFLLDLIVADEERHHDLTARMIAKLKDELAWTRSA
                     GIARRAGESGGRAKRLLASVENFLDAE"
BASE COUNT         1277 a         1696 c         1665 g         1424 t
ORIGIN      
        1 agcggaatgt cgaagccata gatgtgaatg taaacgcctt tgaagcgata caccttgatc
       61 gcctgttcaa gctccagaag cgactctttg atgcgaaatg gattgtagct ggcaagcccg
      121 acgaagcggc tcggatagcg ggtcgtgtat tgcgccactt cttcgagctt ggtgtccata
      181 tacatccact tgttgcggta cgaccacatc ttgcactgag tgatgaaaac cttgtcaacg
      241 cccgcttcgt ccatcttggc gatcatcgcc tcgatgctgt catactgcgg caggccgcca
      301 atggcctttt ccatacggca gatgagctcg ccttttttcg cctcgttcca cttggcgatg
      361 aattcgggcg tcgccacata gtacatgaag tcgattgcct tgataccgtt tcgcatcttt
      421 cttcctcctc tcacccacag ggatcgtttc tcgatctcgc gacgaagacg agccacgatt
      481 cacgaacacg aacatggtgt tcaaaccatc gtcagaccgc cgctcacgct caaagtttgc
      541 ccggtgataa actcagccgc aggagaggtg aagaacagca cagcttcggc gatctcttcg
      601 ggtttggccg gacggcgaaa gggcgtggct ttggtcaccg cttcgataat tttcgcggtc
      661 tgttccgatt ggcttctgac ggcttgcagc agcggggtct ccgtcaggcc ggggcacacg
      721 acattgacgt tgatcttata ccgcgccacc tcgcgagcga tggtctttga gaatgcgatc
      781 acggctccct tggcgccggc atacaccgct tcccaactgg aaccgacccg gcctgcgtcg
      841 gagctgatat tgacgatctt gccgccgcca tgagagatca tctggggcaa tacggctttg
      901 gccgtataaa gcacggactt gaaattgatc gccagcagtt tctcccaggt ctcttcctga
      961 ctttccacga agggctccaa tcgatcccaa ccttggcagt tgacaagaat atcgatctga
     1021 ccgaagcgcg cgagaacttc gttcaccatg cgatcgacgt ctaccttctt ggtcagatcg
     1081 acctggcagg cgattgcgtc cacgccaaga gccttaacct cggtgctgac tttctccgcg
     1141 ttgtctttga caatatccgc gaccgcggtc tttgcgcctt cgcgcgccaa ggccagcgcg
     1201 accgcgcggc cgattcctcc cccacctcct gctatgagag cggtttttcc ttgaagcttc
     1261 atgatgctgc ccttgcctcc tctccgagca cggctgtttt tttatcttcg cgaatcttct
     1321 ccagacgccg cagcgccagc agagcggcgc ccaaagcgcc aaccagttcc ggctcctcgc
     1381 tgacattgat cttacgcttg agtgtctcct ccaacgcctt gaccatgccc ttctgtcggg
     1441 cgacaccgcc gacgaaggtg acctcgtctt ccatgccgga gcgcttgagc aatgctaagg
     1501 cgcgggaagc cagcgagttg tgcacgccgc gcagaatgtt ctcgatagtc tctccgtttg
     1561 agacgtgatt gatgatctcc gattctgcca aaaccgcgca gacgctgctg atggtttgcg
     1621 gcttattagc ctgaatggag agctcgccga cctgatcgat attgacctcg agatatttgc
     1681 aagcgcgttc gatgaagccg ccagcgccag cggcgcactt atcgttggtg cgaaattcgc
     1741 ggactttgcc ggtgtcgcta acgcgcagcg ctcgcgtgct ttgggcgccg atgtcgagca
     1801 cgcaacgagt cttgggaaag aggaaaaccg cgccccttcc gacacaggta atgtcggtga
     1861 tctgcacatc acgaaaagga acgttgtagc ggccaaagcc ggtggtggcg atgtagttca
     1921 ggtcagtctc tttgcgccct gcccgctgaa gcgctccatc caacgcctcc ttggcaatgg
     1981 cggcgaaatc cggccgggtc ttaacgctcg ccttgccgca aatgcgcttt tccccgtcca
     2041 tgatcaccgc cttggtcgat ttagcgccga tatcaatgcc agcgacaaaa gtcatgatgc
     2101 ctccttgagt cagggcaacg gctgagacct taggcggtag gccccgcctg gattgctgtc
     2161 ccctgctgcg tttcgtgcgt cgctttcgta gcgcgctcca gggcgaagat gctcgcgccg
     2221 agggcgccga cgaagtggga atccggactt acgttgagtt tcatgccgag tttttcctca
     2281 agcgcgcgca ccatgccgat gttgagcgaa actccgccgg tgaaagtcac ttcgggttca
     2341 ataccgacac ggcgaaccag cgagatggtg cgggcggcga tggcgctgtg cacaccgcca
     2401 aggatgtcct cggtcttttt gccttgagcg agataggaca tgatgtccga ctcgacgaac
     2461 acggtgcaaa cggtggtcaa gcgcaccggg ttcttagcct gaagcgagat atcgccgata
     2521 tctccgaggg tcatgcccag agcttcggcg gcgctggcga ggaatcttcc ggtgccggcg
     2581 gcgcacttgt cgttcatgac gaagtctttg acgtcgccgt cgatgccgat tttaattcct
     2641 ttggcgtcct gcccgcccat atcgatcact gtgcgggtgc tgggaaagag atagctcgcg
     2701 ccccgcgcat ggcagctgat ctcggtgatt tgcgcgtcac cgaacgtgac tttgtacctg
     2761 ccataacccg tgcccaccac gtaagcgaca tcttcgcgtt tcacctggga gattcgtagt
     2821 gcttcgacga agcatctttc cgcggctctg gtaacatacg cgccggtatc ggtcagagct
     2881 ctggcaacta tctcgagctt gtcgttgagg ataataccct tggtctgggt ggagccaaca
     2941 tctattcccg cggcgtaaat catgctgcgc ctcctgcttc cgctcccgct cgaaccattt
     3001 tcttgtgctc caacgattca aagaaagcgt cgatgcggtt tttgagctgg gcttcggaaa
     3061 aatatcgcgg atcttcgaga tcggattcga tcaatagcgt gggaatgttg agatccttgg
     3121 tgaaatattc gcgcatgtca ccctgtcccg cggaaaagag acggcagctt ttcaccgaat
     3181 gaatcaccaa agcgtcggcg tgccactctt cgacatagcg gcgaatctgc tggtagcgct
     3241 gcaggaagtt gcggttggtg acgttccatt gaagcatgtg gtcggcgatg gattccagcg
     3301 ggtggttgga atcatgccgg aaaccaaatt cccacaaacc gccgacggta gagtaagtcg
     3361 aggccaccgc cacggcgccc catttagaaa acatatctct aaatgttcgc agatagggcc
     3421 aggggggcgg tccttcgata ataaaacgaa agcgctcctc aggcagcggt ccagctccca
     3481 tccgcacttt ttcttccatt tctttaagtg cggtttcgaa aaacctcacg ccctcttcgg
     3541 ttcccctgag acagtaaaga ggcgccatca tggttaccga gtcgaagtag gcgtcgaaag
     3601 gagaaggaat attcttggtc agtcccttga tccgtgacca catctcttca gtctcactcg
     3661 agcggctgac gatttgtttc agccgagcat agtcgagctt ctttcccgtc agcctttcgc
     3721 agatctcgat cacctcttcg agttggcgca cgacgtaact aatgtcctcc gggctcgggt
     3781 ggccggaagg gtcgcgcaaa aatggaatgt cgaggttgta gagttgccca tcgcagtact
     3841 cggctagatg ctcgaaccac tgcaggtaga cgttgcagcc gacgtagttg cagagaatca
     3901 gcgacggtaa agggatggtc gcgaaaggag tcttgcggtc gccgagaaac atcccgacat
     3961 cggctttcac ataggcgcag ttgtccatgg aataacccaa ctcttcggcc ttctggatga
     4021 aaggcagggc gctcttcttg gccgctaatt gcaaggcatc gatctccggg tagatcggga
     4081 tcaagtcaaa agcctgaatc agttccacag ggttgccgct gatcaacatg tagacggctt
     4141 tgccctcgcc ctgttccgcg gcccgggtga cgcgctcgaa atagcgcccc atcagctcct
     4201 tttgcagcag atgcatttcg ccctgatact gagttttttc agctacggct gttgcggcca
     4261 taaatttctc ctaatcgaaa agcattgatt cgacgaaagt ctcgacctcg gtgcgcgcct
     4321 tatcgaagat ccacatcttt tcctcgaatt cgagggacag gtgcggaata ccctctttct
     4381 cgagcgcacg tttatatagc gcgtagtcga acaacgccgg ctcgcagaat ttcgccgcca
     4441 ggatcaacac cgcgtcggcg ccggtatgtc taaatttatc gatcaaatgc ttggatttgg
     4501 gctcgcgcgt gtcgtgtttc actcccgaat aaacgctctg attaaggtaa gcgttggcga
     4561 gattgcgcaa gggatcgccg tcggtgggaa tatcccccag gaaccagcgc cagccgataa
     4621 ggaagtcatc gtcgagtatg tagcacccgg ccgattcgag tccgtcgagc agttcgatcg
     4681 gcggctgctc gcagaaagag ccttctaaaa ccactctgat cctgtccttg ggcttttcgt
     4741 tgcgccggcg cagttgcggg atcgcctctt caagaatcgc gatatgttct tctggcggca
     4801 gtaacgtgcc gatccgcgtc aagacgtaac attcgacggt actgagattc tgtggggtct
     4861 ctcgacgaat agcatacaga tcccggatca ttcgccgctg tcgattgtaa agcgctatgc
     4921 tctggctgag cccgtcgtca gtgattttct tgccggatag cttttcataa gtctcgccaa
     4981 ttcttttgta ctcggccatc aggtaatcga tcgtatgcgg cgaagtcatg ttctgcggaa
     5041 agtgaatgta ctcgaccatt agatcgggaa aattccgtgt gaagacgctg gccagattgc
     5101 gcgccggatc acagatcgag tgaaaaacga tgccatcgag aaatttcagt ttatcgatca
     5161 gaccgagttc cagagtgctc ttgacgatgg aacagataaa cgattgaaaa cgtgagtcag
     5221 catgagcgat ctctagccgg ttgcctgcgc cgatgacccc gacaggcagc attcccgcag
     5281 cgtgaatgag ttcgaatgga gtatagacgg gatagcatcc gactagcttt ttatcgggat
     5341 gcatcttctt ccaatccgag gcaagttcga gagggtaagt ctcgagcgga tcgcgatagc
     5401 gagaaacaag attctcgacg gttgtcattg ggtacctctt tactcggctt tactcggcgt
     5461 ttacaaggag taccgcaata cggatgccac caatattcta ccggttgctc tgcagcgaag
     5521 atcgaatctt cgcaaccatc ggaaagctac tccagataat tctcaattct cagaatgtgg
     5581 atgtccactc gcccattgcg tcctatggca tttgctttgc tctgttggtt gatgagttgt
     5641 cgccgaagat cgttgccaag ggaggagaac tgtcatggct cgagcagtgc gatccggatc
     5701 cattgcgatc atcgccggtc aacgggcaga agatgcgacg tggggcatac ccgagtcgcc
     5761 ggagttctca tcggtccagc gcctcatgaa cgaatttcaa tcccatgccg ctcatgaaga
     5821 gcggtggctt tcgagctatc aagagatcgc gaaagaaacg atcgatcctc agatccggtt
     5881 tcttctcgat ctaatcgtcg ccgatgaaga gaggcatcac gatctcacgg cgcggatgat
     5941 tgcgaagctc aaagatgagc tggcgtggac tcggtcggca ggaatcgccc gcagagccgg
     6001 tgagagcggg ggaagggcca agcgtctgct tgcgtccgtc gagaactttc ttgatgcgga
     6061 gc
//
LOCUS       DASUTG010000842         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_724120, whole genome shotgun sequence.
ACCESSION   DASUTG010000842 DASUTG010000000
VERSION     DASUTG010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724120"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF089_19595"
     CDS             <1..386
                     /locus_tag="VF089_19595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176220.1"
                     /translation="EAFKRANCPSLWSGGVRPFRPADFTNFGWSYNVTVRARANDINS
                     LANFSLSIESWHNDAHMAVGQAFGIEDDMMNPRVNIYYREFWRLHYFINDRFLAELRR
                     YDSSGTVTQKMNRLEQNQHTNLYRI"
     gene            802..1029
                     /locus_tag="VF089_19600"
     CDS             802..1029
                     /locus_tag="VF089_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176221.1"
                     /translation="MARIFMRRFLAACLLLLLVGCGDKAKDLYDTAQFEEKQNNRPHA
                     TKLYRQIVEDYPDSPYANQAKNRLAELKKAR"
     gene            complement(1765..2712)
                     /locus_tag="VF089_19605"
     CDS             complement(1765..2712)
                     /locus_tag="VF089_19605"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX6176222.1"
                     /translation="MKLTASARLGRDLPGWLSGVMVLGTMAAVLWLETRRPLRRQHHN
                     KIKRDARNLGMAAMSAVAISRLESPLVRRLSHTVHRRRWGIVKTLGLPGWLEVGLAVV
                     LMDYTLYVWHVLTHKAPLLARFHRVHHADLDMDASTAIRFHFGEMILSVPWRAAQVLL
                     IGTAPLSLSMWQTLTTMAILFHHSNSRLPHALEKRLCRIIVTPRMHGIHHSIILEETD
                     ANWGTIFAIPDYLHGTIRLNIPQDNVVIGLPVLRNEDELTLPKLLTMPVTAGDPDEQH
                     PPELNTRSERQELPRTVLANGTERTAGYVPEAAAAPARS"
     gene            complement(2709..3242)
                     /locus_tag="VF089_19610"
     CDS             complement(2709..3242)
                     /locus_tag="VF089_19610"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HEX6176223.1"
                     /translation="MATHKSSVQRDLMIGAGAGLLGMLAVGPVNAFLSRFISEEQYRK
                     EHAVRDASPHDLAAQKIAERFGGNPPSEQAQLLSQLAFGAAYGLAWGMIYAMLRRSAP
                     PISRFAGLPFGSAFFFLCDGVLAPLFRMSPPITRLPWQPNTKEMLNHVAWTAAAELAH
                     RAADRKLRTNDREDAWS"
     gene            3280..>3469
                     /locus_tag="VF089_19615"
     CDS             3280..>3469
                     /locus_tag="VF089_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176224.1"
                     /translation="MNTKTNAIAVPPKRRSRRFAGRLSSNRQSFERRMCRVREASCLS
                     CRKIETALKEQREFDLTAQ"
BASE COUNT          793 a          948 c         1006 g          722 t
ORIGIN      
        1 ttgaagcgtt caagcgagcg aactgcccat ctctttggtc cggaggcgtt cgcccattcc
       61 gtcccgcaga ttttacaaat ttcggctgga gctataacgt gacggtacgt gcgcgtgcca
      121 acgatatcaa ctcactggct aacttctcac tgagcatcga gtcatggcat aatgacgcgc
      181 acatggcggt cggacaagcg tttggcatcg aggatgacat gatgaatccg cgagtaaata
      241 tctactacag agaattttgg cgtctccact actttatcaa tgacaggttc cttgccgagc
      301 tgcgtcgcta cgactccagc ggaaccgtca ctcagaagat gaatcgtctt gagcagaatc
      361 agcataccaa tctataccgt atttagcttt accgcattta gcgttgacaa aggcttgtca
      421 ggggtcggac cgaggcgtgt ctgagcagtt tcagtgttct cacccgaggc gatcggcttt
      481 tcgaatgaga aattcaaccc ggcgtgtttc actccgcttg aacgggggag gcggtttagc
      541 cagcatgcac gaaccctacg agagacgtgc ccaggcgaga aaatagcgat tggcccgtca
      601 acgtttcatt gaaggttagt tgtcggtcct aaagaaatga acgataagcc ttaaaccatt
      661 tttacctatg ccgtatttaa gaacttcccg atccgggaca ggagctccca ccctccttgt
      721 tcaccgccaa ataaactcaa cacgaagcct aacacagcta gtcccaggct catcaatcaa
      781 tatggtagtc tgacacaaca catggcgcga atttttatgc gacgatttct tgccgcctgc
      841 ctgctcctgc ttctcgtcgg ttgcggcgat aaagcgaagg atttatacga taccgcccag
      901 ttcgaagaga agcagaacaa caggccgcac gccacgaaac tttatcggca gatcgtagaa
      961 gactatcccg attcccctta cgcaaatcag gcgaagaatc gtttggccga gttaaagaaa
     1021 gcgcgttaga tattcacgca ggatccccgt agttactcgg aataataaaa cagcgaggta
     1081 cgcactagtc ttcgggatcg ctgtgatttt tccggaccaa tgaattgccg cttgcctcta
     1141 acatttcgcc ctgtagagcg cagcggctgg cctcaccggc aagtagcgat gtaactacac
     1201 tgatgggctt tagctgaaat cgtcaagctg tcggcgcagt ggcagggcgg tgtgccggct
     1261 cttcgcgcac tcattcgaga tcgagagcga ttatttccgc cgcccgatga cgttcgaaac
     1321 cctgttccgg gagtgcaagc agcgaacgtt cagggtgtga cgcgaagcgg ggaagccaat
     1381 cacggttagg ccgaagaaga ttttaatgag tatttgtcaa caaaatatct agagattcga
     1441 agacagcgcg gacttgagga tgtctatgag ttactcggga gaaatctcac tggagctttc
     1501 gaaattcggt aaacgatagg cttttcatcg atcgcctcct ctcagtcgag actgtacagt
     1561 actgcacatt ccatctgcaa gtccggccgg gtctaggagc agaaaagggg gaaccggcta
     1621 tttttccgtt gaagtgggac gggatggaag ccctgcgcgc cgacaattgt cagaggacag
     1681 cggccggtac gccgcccagg cagtagacga catcaaaccc acctgacaga tttcgcagcg
     1741 cttctgcctg aaaacacaat ccgatcaaga gcgcgccggc gctgccgccg cttccggaac
     1801 atacccggcc gtacgctccg tgccgttggc gagaacggtt ctcggaagct cctggcgttc
     1861 gcttctggta ttcaattccg gcgggtgttg ctcgtccggg tctcccgcgg tgaccggcat
     1921 cgtaaggagc ttcggcagcg tgagttcgtc ctcattgcgg agaacgggaa ggccgatcac
     1981 cacgttgtcc tgcgggatgt tcagacggat cgtgccgtgc aggtaatccg gtatcgcaaa
     2041 gatggtgccc cagttggcat cggtctcttc gaggatgatc gaatgatgga tcccgtgcat
     2101 gcgcggcgtc acgatgatcc ggcacaatcg tttttcgagg gcatgcggca gtcgggaatt
     2161 cgaatggtga aagagaatgg ccatcgtggt gagcgtctgc cacatcgaga gcgagagcgg
     2221 agccgtgccg atcagcagca cttgcgccgc gcgccaggga accgacagaa tcatctcgcc
     2281 gaaatggaag cggattgccg tggatgcgtc catgtcgaga tcggcatgat gaacccgatg
     2341 gaaccgggca aggagcggcg ccttgtgcgt caggacatgc cagacgtaca gcgtgtaatc
     2401 catgagcacg accgcgaggc cgacctccag ccagcccggc agcccgaggg ttttgacgat
     2461 tccccagcgg cgccgatgga ccgtgtgact gaggcggcga accaatggcg attcgaggcg
     2521 gctgatcgca acggcgctca tagccgccat cccaaggttc ctcgcgtcac gtttaatctt
     2581 attatgatgc tggcgccgca gcggccgtct ggtttccagc cacagaaccg ccgccattgt
     2641 gccgagcacc atcacgccgg atagccatcc cggaaggtcc cgtccgagcc gggccgatgc
     2701 ggttagcttc atgaccaggc gtcctcccga tcgtttgtgc gaagcttgcg gtcggcggcg
     2761 cgatgggcca gctccgcggc ggcagtccag gcaacgtggt tgagcatttc ctttgtgttg
     2821 ggctgccagg gtaaacgggt aatcggcggc gacatgcgga ataacggcgc gagaacaccg
     2881 tcgcacaaga agaagaacgc agatccgaat ggcaagccgg caaagcgtga gatcggcggc
     2941 gcagatcgcc tgagcatggc gtagatcatt ccccaggcaa gaccgtaagc ggcgccgaac
     3001 gcgagctgac ttaggagttg cgcctgctcg ctgggtgggt tgccgccgaa ccgttccgca
     3061 attttctgcg cggcgagatc gtgcggtgag gcgtcgcgga cagcgtgttc tttgcggtat
     3121 tgctcttccg aaatgaagcg ggaaagaaag gcgttgaccg gaccgaccgc gagcatgcct
     3181 aagaggcccg cgcccgctcc gatcataaga tcgcgttgca ccgatgattt gtgagttgcc
     3241 atagatccct ccgtgattcc gccccaactg caggtagtga tgaataccaa aaccaacgca
     3301 attgcggtgc cgccaaagcg ccgctcgcgc cggtttgcag ggcggttgtc gagcaaccgg
     3361 caaagctttg aacgacgaat gtgccgagtc cgggaagcga gctgcctttc gtgtcgcaag
     3421 atagaaacgg ctcttaagga acagcgagaa ttcgatttaa ccgctcaat
//
LOCUS       DASUTG010000843         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_871627, whole genome shotgun sequence.
ACCESSION   DASUTG010000843 DASUTG010000000
VERSION     DASUTG010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871627"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VF089_19620"
     CDS             complement(<1..990)
                     /locus_tag="VF089_19620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176225.1"
                     /translation="MAEYRIIDADGHVMELDGELREFIGPPYKDLEWHQSYSFWPGLT
                     MDGYLRSLRQKGGWAGGGEGPKANHWLDFLDKNQIELTVLYPTQGLTHGAIQDKDWAI
                     CMARAYNDWLYHKFMAVSPRLVGVALLPIQDISEAVKELRRCVNDLGMVGAVLPAVAP
                     AGRLYSGPEFYPLWEEAQKLDVPVSTHGGLSMPSLGLDMVNNFTIGHTLEHPFAQIRQ
                     FTALMFEGIFELFPNLRFGCIECGVGWVPYMMDRMDEEQERKGHYSPRCKMKPSEYVK
                     KGNIFFAVEVEESALPLAAQVANDNALIWASDYPHERDQRDFSQDIPSLINRKD"
     gene            complement(1073..2032)
                     /locus_tag="VF089_19625"
     CDS             complement(1073..2032)
                     /locus_tag="VF089_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176226.1"
                     /translation="MAKLKLTIACDKYDYLQPLRQGQVEVEGIDLNLITVESGIRHER
                     MFRYGEYDACEFSMSSYLVARGQDVDWLQAIPFFPRRMWGHKFCFIRCGSGIKKPADL
                     KGGRIGLRSYENTLALVTKGMLMNSYDLSVTDVTWVIVNKEHVGFKLPANIKVEFVEG
                     QRKLEDLLVEGKVDAEVEPDLPQRWIRNEGTVERLFPDFEKEEKDYYRRTKVFPIMHP
                     VVIKKEILNSDPWVATSLYEAFVASRQAYNEFMEQPHRLSFAWARSYLEEERKFFGKD
                     PFYQGFRENRHDVQNLIKFAGQQGMLGRQLTVEELFAENTHST"
     gene            complement(2194..>2469)
                     /locus_tag="VF089_19630"
     CDS             complement(2194..>2469)
                     /locus_tag="VF089_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176227.1"
                     /translation="SFADLATAAGNDHGFVFEAHDRILLWEIMKDESRKLKRMLARRD
                     CEDGNLNKYFLMRLISVFSFHHLALTNVVLPLTPPHRPVNEPAVSLT"
BASE COUNT          552 a          664 c          640 g          613 t
ORIGIN      
        1 atctttgcga ttgattagac taggaatgtc ctggctaaag tcgcgctggt cgcgttcgtg
       61 cggataatcg gaggcccaga tgagcgcatt gtcgttggcg acctgcgccg ccagaggtag
      121 cgccgactct tcgacttcca cggcaaagaa gatatttcct ttcttgacgt actcgctggg
      181 tttcattttg cagcgcggcg aatagtgacc tttgcgctct tgttcctcgt ccatccggtc
      241 catcatgtaa ggcacccagc cgacgccgca ttcgatgcag ccgaagcgca ggttgggaaa
      301 cagctcgaaa atcccttcga acatcaaggc ggtgaactga cgaatctgag cgaacggatg
      361 ttccagggtg tgtccgattg taaagttgtt caccatatcg aggccaagcg acggcatgct
      421 gagcccgccg tgggtcgaaa cgggaacatc gagtttttgc gcttcttccc aaagcgggta
      481 gaattcagga ccgctgtaaa gccgtccggc aggagcgacc gcaggtagca cggcgccgac
      541 catgccgaga tcgttaacac aacgacgcag ttctttaacc gcttcggaga tatcttggat
      601 cggcaaaagc gcgacaccga cgaggcgcgg gctcactgcc atgaatttgt ggtagagcca
      661 gtcgttgtac gctctcgcca tgcagatagc ccagtcctta tcctggatcg ctccatgagt
      721 gagcccctga gttgggtaga gaaccgttag ctcaatctgg ttcttgtcga ggaagtcgag
      781 ccaatgattt gccttgggac cctcgccgcc gccggcccag ccgccttttt gacgcagtga
      841 acgcaggtag ccgtccatgg tgaggcccgg ccagaacgag tagctctgat gccattccag
      901 atctttgtaa ggtggaccga tgaattcgcg cagctcgccg tcaagctcca tgacgtgccc
      961 atctgcatcg ataatgcgat actcagccat gttagtctcc tctctcgatt gtgtcgctca
     1021 cattattcca aaaacgagcg atcggactct tttctattct tatgacaatg cgtcacgtgc
     1081 tgtgggtatt ctcggcgaaa agctcttcaa ccgtcagttg acggccgagc attccctgct
     1141 ggccggcgaa cttaatcaga ttctgcacgt cgtgacggtt ttccctgaag ccctgataga
     1201 aggggtcttt accgaaaaac ttccgttctt cttccaaata cgagcgtgcc caggcaaagc
     1261 tgagccgatg cggttgctcc ataaactcgt tgtaagcttg acgtgacgcc acaaatgctt
     1321 cgtagaggct ggtggccacc cagggatcac tattgaggat ctcttttttg atgacaactg
     1381 ggtgcatgat cgggaaaacc ttggtccgcc ggtagtaatc tttctcctct ttttcgaagt
     1441 ccggaaacag tcgctcgacg gttccctcgt tgcgaatcca tcgctgcggc aaatcgggtt
     1501 cgacctccgc atcgaccttg ccctcgacca acaaatcttc cagcttacgt tgcccttcga
     1561 cgaattccac tttgatgttc gccggaagtt taaaaccgac atgttcttta ttgacaatta
     1621 cccaagtgac atcagtaacc gatagatcgt agctgttcat cagcatgcct ttggtaacca
     1681 gggccaaggt gttttcgtag ctgcgcaagc cgatgcggcc gcctttcaaa tccgcaggct
     1741 tcttgatgcc ggaaccgcag cgaatgaagc agaatttgtg cccccacatg cggcgcggaa
     1801 aaaagggaat ggcttgcaac caatctacgt cctggccgcg cgcgaccaaa taggaggaca
     1861 tcgagaactc acacgcgtcg tactcgccgt atcggaacat gcgctcgtgg cggatgccgc
     1921 tttcaaccgt tatcaagttg aggtcgatgc cctccacttc gacctgtccc tggcgcaatg
     1981 gttggaggta gtcgtatttg tcgcatgcta tcgtcagctt taatttggcc atatgatctc
     2041 ctggcggtga aaaaacttag atgctttccc ttgtgctcgc cgaatctaaa ttcgtggccg
     2101 tgtctacgtg ttcgtgttcg ttcggaaaac tctcatcacg ttcacgatca cgaacacggc
     2161 tttatcagct tcgatcatag gtgcggcgcc gcctcaagtc aatgaaacgg caggttcgtt
     2221 gacaggccgg tgaggcggtg ttaagggcag gacaacattt gttaaagcta aatgatgaaa
     2281 gctgaaaacg gaaataagtc gcatcaggaa gtatttgttc agatttccgt cttcacaatc
     2341 tcttctagca agcattcgtt tcagctttct gctttcatct ttcataattt cccataggag
     2401 gattcgatca tgcgcttcaa agacaaatcc gtgatcatta ccggcggcgg tggcaagatc
     2461 ggcaaagct
//
LOCUS       DASUTG010000844         6585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1166800, whole genome shotgun sequence.
ACCESSION   DASUTG010000844 DASUTG010000000
VERSION     DASUTG010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166800"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="ccsA"
                     /locus_tag="VF089_19635"
     CDS             <1..196
                     /gene="ccsA"
                     /locus_tag="VF089_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX6176228.1"
                     /translation="SWEPRQISSGIAWLFYGALLHGRITAGLRGKKAAFLTIVGFTVV
                     LGYFLWGDAIFPGRHGGRFE"
     gene            200..1480
                     /gene="hemA"
                     /locus_tag="VF089_19640"
     CDS             200..1480
                     /gene="hemA"
                     /locus_tag="VF089_19640"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6176229.1"
                     /translation="MHTEIFIVGLNHRTAPIEVRESVAVEKSQITEALGRLRTILALR
                     EGVILTTCNRVEVVAAAPEADTAMQAVRVFLDQQRSCSNNGSLSDHLYTYRGTDAVRH
                     LFRVAASLDSMVVGEPQILGQLKEHYHLAQKAGTVGAVLHRLFHRSFFVAKRVRTETG
                     IASRAVSVSSVAVELAGRIFDRFEEKTVMLIGAGKMGDLMARHLQRCGVKNIMVTNRT
                     FERAVELAEKIRGNPIRFDDFPRYLQLADLVIGCAGASEVMVDAGMVEKVLRERKQAA
                     MFFIDIGDRRNFDPGINAIDNAYIYNIDDLKSVAEENLNERSSEAEKAECIVQEEVGS
                     FIAWLSSLEQVPTIAALVEKLELIRQKEISKSLSGGLKDLSERQKQAMEDMTAAMMKK
                     FLHAPIMHLKQNTDSEYELMYIAALKRLFDLEDQ"
     gene            1534..2451
                     /gene="hemC"
                     /locus_tag="VF089_19645"
     CDS             1534..2451
                     /gene="hemC"
                     /locus_tag="VF089_19645"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6176230.1"
                     /translation="MIGFRIGSRGSALALAQADWVKRKLEQTGLKVEIIVIKTSGDRF
                     VDRTIQSLGGKGIFTKEIEDALLSGTIDLAVHSMKDLPTELPKGLAIAAVPQREDARD
                     VLVSRENIPLKDLPKGAQLATGSLRRRSQILNYRSDLTMIPIRGNVDTRLQKLFDQGL
                     DAIVLAAAGLKRLGRKERITEYLTPEICLSAVAQGALAIETRDDGPSRQSVAFLHHDP
                     SFTEVTAERAFLRKLGGGCHVPVAARAFMEGELLNIMGMVAEPDGRRLCRDRIAGPKE
                     QAAALGTELADRLIRAGAGEMLAGVVTGA"
     gene            2507..3301
                     /locus_tag="VF089_19650"
     CDS             2507..3301
                     /locus_tag="VF089_19650"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6176231.1"
                     /translation="MSGKRIVITRPRKQAGELKRRLTELGAEVIEFPTISVEPPGDYE
                     PMDRAIAQLHQYDWLIFTSVNGVQSFFDRLRHFGNDSQDIEHLKVVAIGPETAARLRD
                     EGVHVYLVPAKYQAEGILEGLNAAEIRGRRILMPRAARAREILPQTLRQWGASVDVVQ
                     AYETVLPHRRSSLHKLFQKQIDVITFTSSSTVENFIRLLGGEDPNKVLQEVIVACIGP
                     ITARTAMDSGLRVDIISSEFTIAGLVAAIVRFYESADTENQKRSRS"
     gene            3318..4288
                     /gene="hemB"
                     /locus_tag="VF089_19655"
                     /pseudo
     CDS             3318..4288
                     /gene="hemB"
                     /locus_tag="VF089_19655"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
     gene            complement(4364..4624)
                     /locus_tag="VF089_19660"
     CDS             complement(4364..4624)
                     /locus_tag="VF089_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176232.1"
                     /translation="MLAEKRGDAAAPEIADWANRVARRIDEQVAVGVDYDGDSNTFLL
                     RLTKGSRVLLFRLSESQVHVSGREAECERTLQRKIKDLWNLI"
     gene            complement(4642..5652)
                     /locus_tag="VF089_19665"
     CDS             complement(4642..5652)
                     /locus_tag="VF089_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176233.1"
                     /translation="MKLFRAYAAAVLSVACFVANPSLAGAQQARINVGYSAISADQLP
                     AWVAKETGIFAQNGLDVQLIFFTGGTTAILALVSGDVPITQVSGPGLVNSVLAGSDAV
                     FIAAGMTSLNYVLMGKAGVKTAEQLKGGTVAISRFGSATDSIARFALQKIGLTPGKDV
                     TLVQVGSGPDRLTSVLSGRVTAAVINPPSNFIAEKKGLSVVADVAQMGLVFQHTGAAT
                     TRKYIRENPDIVRRYVKSHVEAVHRIYNDKEATVKTLGKYMGSNVDREILEKSRENIL
                     TEALLPKKQIPSLEGLQTVLDDIGQRDPRAKAAKPEQFVDFTFIRELEQSGFIDGLYK
                     KK"
     gene            complement(5799..6347)
                     /locus_tag="VF089_19670"
     CDS             complement(5799..6347)
                     /locus_tag="VF089_19670"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6138..6140),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6176234.1"
                     /translation="MKSTTKIMFSLAFAIAVVVGANTAARALEVGDKAPDFELESSKG
                     GKLKLSSLKGKNVLINFYVLDFSPTUIKDLQASGSDNYQQFQAENTEVLGMSVNAPFS
                     QKAFADFAKINYPLLSDASGKTMTMFGVFDQERRQAKRSYVIIDKEGVVRYVNVRPSN
                     GPKDLLSTEELLAEVKKVNKAS"
BASE COUNT         1521 a         1640 c         1855 g         1569 t
ORIGIN      
        1 gtcgtgggaa ccgcggcaaa tatcatcagg catcgcgtgg ctgttttacg gcgcgcttct
       61 gcacgggcgc atcaccgccg gtttgagggg caagaaggcg gcctttctga ccatagtcgg
      121 tttcaccgtg gttctcggtt attttttgtg gggagatgcg attttcccgg ggaggcatgg
      181 gggtcggttc gaatagctcg tgcatacaga aatcttcata gttggcctca accatcgtac
      241 agcgcccatc gaggtaaggg aaagtgtcgc ggttgaaaaa tcccagatca cggaagcgct
      301 cggacggctc cggaccattt tagcgcttcg ggaaggcgtg atccttacca cctgtaatcg
      361 ggtcgaagtg gttgccgccg ccccggaggc tgataccgcc atgcaggcgg tcagagtatt
      421 tctcgaccaa caacgatcat gttcaaataa cggcagcctt agcgatcatc tttatactta
      481 tcgcggtacc gacgctgtgc ggcacttatt tcgcgtcgcc gcaagtcttg attccatggt
      541 agttggcgag ccccagatcc tcggccaatt gaaagagcac tatcacctgg cgcagaaggc
      601 cgggaccgtc ggtgcggttc tccaccgcct gtttcacagg tctttttttg ttgccaaacg
      661 agtccggact gaaaccggca tagccagccg cgctgtctcg gtaagctcgg ttgcggtgga
      721 actggcaggg agaatttttg accgtttcga agagaaaacg gttatgttga tcggcgccgg
      781 caagatgggc gatctgatgg cgcgccatct ccagcgttgc ggtgtaaaga atattatggt
      841 gacaaaccgt acctttgagc gcgcagtaga attggcggag aagatccgtg ggaatccgat
      901 tcgattcgat gatttcccgc gctatttgca attggcggat ctggtgatcg gatgcgccgg
      961 agcgtcggag gttatggtgg atgccggcat ggtggagaag gtcctgcggg agcgcaagca
     1021 ggcggcgatg tttttcatcg acatcggcga ccgaaggaat ttcgatccgg ggatcaacgc
     1081 gatcgataac gcttatatct ataacatcga cgatctgaag agcgtggcgg aagaaaacct
     1141 aaacgaaaga tccagcgagg ccgagaaagc tgagtgcatc gtgcaggaag aggtcggcag
     1201 ttttatcgcc tggcttagtt cactagagca ggtgccgacg atcgcagccc tggtagagaa
     1261 gcttgagctt atacggcaga aagagatcag caaatctttg agtggcggtc ttaaggattt
     1321 gtccgaaaga caaaaacaag cgatggagga catgaccgcg gctatgatga agaagttttt
     1381 gcatgcgccg attatgcatt tgaagcaaaa cacggatagc gaatacgagt tgatgtacat
     1441 cgcggcgctg aaaaggctgt ttgacttgga ggaccagtga ggcgcgacgg atgttctaag
     1501 acttactggc ggaattagcg aaagtcttgg ccaatgatag ggtttaggat cggtagccgt
     1561 ggtagtgcgc tggcgttggc gcaagctgat tgggtgaaaa gaaaactcga gcagacgggt
     1621 ttgaaggttg agattatcgt gatcaaaacc agcggcgacc gatttgtcga tcgaacgatt
     1681 cagtccctgg gaggcaaagg aatattcacc aaagagattg aagatgcgtt gctcagcggg
     1741 acgattgacc tagccgtaca ttcaatgaag gacttgccaa cagaactgcc caaggggttg
     1801 gccattgccg ccgttcctca aagagaggac gctcgcgatg ttttggttag cagagagaat
     1861 attccgttga aagatttgcc gaagggcgct cagcttgcga ccggcagtct tcgccgcaga
     1921 tcgcaaattc taaactatcg atccgatcta accatgatcc caatcagggg gaacgttgat
     1981 acccgcctgc agaagctatt cgaccaagga ctggatgcta tcgtcctggc ggccgccggg
     2041 ttgaagaggc tcggcagaaa agaacggatt acggaatact taacgccgga aatctgtttg
     2101 agcgctgtcg cccagggagc attggccatt gagactcgtg acgatggacc gtcgcgtcaa
     2161 tcggttgctt tcctgcatca cgatccgagt tttacagaag tcacggcaga acgagccttc
     2221 ctacggaagc tcggcggtgg ctgtcacgtg ccggtggcgg cgcgcgcctt catggaaggt
     2281 gagcttctga atatcatggg catggtcgcc gaacccgatg gccgccgtct ttgtcgcgat
     2341 cggattgccg gtcctaaaga acaggcggcc gcgctcggaa ctgagttagc tgatcgccta
     2401 atacgggctg gagcagggga gatgctggcg ggagtagtaa cgggcgcgta gcgagtgcag
     2461 tatggtagcc aacgaatcct caaagcgtcg aggtgatatt ttagtattgt cgggaaagcg
     2521 aattgtcatc actcggccgc gcaaacaagc aggtgaacta aagcgtcggc tgacggagct
     2581 aggagcggag gttatcgagt tcccgactat ctctgttgag ccgccaggcg attatgagcc
     2641 gatggatcgc gccatcgcgc agctccacca atacgactgg ttgatcttta ccagcgtcaa
     2701 cggtgtgcaa tcgttctttg atcggctgcg tcattttggc aatgattctc aagatatcga
     2761 gcatctgaaa gtggttgcta tcggcccgga aacggcagcg cgtctgcgag atgaaggcgt
     2821 tcacgtttat ctggttccgg ccaaatacca ggccgaaggg attcttgagg gattgaacgc
     2881 ggccgagatt cgcggccgcc gaatcctgat gccgcgcgcc gccagggccc gagagatatt
     2941 gccacaaact ctgcgtcagt ggggcgcctc cgtggatgta gttcaagcct acgaaaccgt
     3001 gctgccgcac cgccgatcca gtttgcacaa actatttcag aagcaaattg acgtgattac
     3061 gtttactagc tcaagtacgg ttgagaattt tatccgttta ttgggagggg aggatcctaa
     3121 caaagtattg caggaagtga tcgttgcctg catcggaccg atcacagccc gtactgctat
     3181 ggatagcggc ctgcgggtgg atattatttc gtccgaattt acgatcgccg gtttagtcgc
     3241 tgcgattgtc aggttttacg aatcggctga tacggaaaat cagaaacgca gccgatcgta
     3301 gagagctgga gcagagcatg ccgtttcccg cttatcgacc tagacggtta cgcaaaagcg
     3361 aaaagctgcg gcaactgatc cgcgagacca cgcttacagc gagcaacctt atttacccgt
     3421 tgtttgtcgg accgggaacg aacgtctacg cccgatttct tcgatgccgg gcgttgccca
     3481 gctgtcggtg gatcgggcgg ttctcgaatg ccgcgaggct gccgctctcg gaataccggc
     3541 ggttattcta tttggcatcc ccgaaactaa ggacgcggtc ggctcggaag cctctaatac
     3601 tgatggcgtg gtgcagcaag ccgtgcgagc gatcaaggac aatgttcccg agttgttagt
     3661 cgttaccgat gtttgcctct gtgaatacac cgatcatggc cattgcggct taatcaaaaa
     3721 cggcgctgtt gataatgaca gcaccttgga acttcttgcc gaggaagcac tttctcatgc
     3781 gcaggccggg gcggacatcg tcgcgccctc ggatatgatg gatggcaggg taggcgcgat
     3841 ccgtcgggtt ctggacagca acggcttcga tcaaattccg ataatggctt acgcggccaa
     3901 gtatgcttca ggcttctacg gcccgtttcg cgaagcggcg gaatcaacgc cgcagtttgg
     3961 cgatcgccgc tcttaccaaa tggatcctgc caataccgat gaagcgttgc gggaagtcga
     4021 actcgacatt caggagggcg cggacattgt catggtcaag cccgcgatcg cctaccttga
     4081 tattatttat cgagtaaaac agcagtttgg ttatccggtt gcggcttaca acgtcagtgg
     4141 ggaatactca atgatcaaag cggccggtat caaaggttgg attgatgaag aaagaatcat
     4201 gctggaggtt cttttatcga tcagacgcgc gggtgcagat atgatcttga cctatttcgc
     4261 taaagatttg gcgcgctcct tagcttagat gttttgcaag cagtccttcc tgagtctcag
     4321 taagctatag atctcgaaaa agatgcatgg caaaccgtat ccgctaaatt aaattccaca
     4381 gatccttaat ctttcgctgc agtgtgcgct cgcactcggc ttctctcccc gagacgtgaa
     4441 cctgagattc cgaaagtcgg aacaggagca cccgcgagcc ttttgtcagc cgtaagagaa
     4501 acgtattgga atcgccgtcg taatcgacac ctacggccac ttgttcatct attcgccggg
     4561 caacacgatt ggcccaatcc gctatctcag gagccgccgc atcgcctcgc ttttcagcca
     4621 acatcaacgg ccgggttgcc gttatttctt cttgtagagc ccgtcgataa agccgctctg
     4681 ctcgagttcg cggatgaaag tgaaatcaac gaattgctcc ggcttagctg ctttggcgcg
     4741 gggatcgcgt tgaccgatat cgtcaaggac ggtttgcagg ccctcgagac tggggatctg
     4801 tttttttggt aataaagctt cagtcaagat attctcgcgg ctcttctcca ggatctcgcg
     4861 gtcgacattg cttcccatgt acttgcccag ggtttttacg gtagcctctt tgtcgttata
     4921 gattctgtga acggcctcaa catgtgattt gacatacctg cggacaatgt cggggttctc
     4981 acgaatgtat ttccgggtcg ttgccgcgcc ggtatgctga aacaccaacc ccatctgggc
     5041 aacgtctgcc actacgctca gacccttttt ctcggcgatg aagttagaag gaggattgat
     5101 aaccgcggca gtgactcgtc cgctcaagac agaggtgagc cggtctggtc cgctaccgac
     5161 ctgcaccaat gttacgtctt tcccaggagt gagaccgatc ttttgcagcg cgaaacgggc
     5221 aatcgaatcg gttgccgaac cgaaacggct gatggctacg gtgccgccct tgagctgttc
     5281 ggcggtttta actccggctt tgcccattaa gacatagttg agcgatgtca ttcccgcagc
     5341 gataaacact gcatctgaac cggccaaaac actgttgacc agtcctggtc cggacacctg
     5401 agtgatcgga acatcgccgg aaaccagcgc aaggatagcc gttgtgccac cggtgaaaaa
     5461 aatcaactgg acatctagac cattttgagc gaagatcccg gtctccttgg cgacccaggc
     5521 cgggagttga tcggcgctga ttgcgctgta gcccacgttg attcgagcct gttgtgcccc
     5581 cgccaaagag ggattagcta caaaacaggc tactgacaaa actgcagcag cgtacgctct
     5641 aaaaagtttc attgggtcct ccagaagaat ctacgtcaat agtactagac cacgaatttt
     5701 caaacaagca cgccttaccg gcgcagaggc ttgcttgttt ttactggata catctagagt
     5761 ttcaagtcat agtggtcttg gcgatgttgt gggcgattct aactggcttt gttgaccttt
     5821 ttgacttcag caagcaactc ctcagtggag agaagatcct tcggcccatt gctcggccgg
     5881 acgtttacgt aacgtaccac gccttcttta tcgataatca cgtaggaacg cttggcttga
     5941 cgtctttctt ggtcgaatac gccgaacatt gtcatggttt ttccgcttgc gtcgctaagc
     6001 agcgggtaat taatcttggc gaaatcggcg aaggccttct gtgaaaacgg agcgttaacg
     6061 ctcatcccca agacttcggt attttctgct tggaactgct gataattgtc cgaaccggac
     6121 gcttgtagat ccttaattca ggtaggacta aagtccagca cgtaaaagtt gataagaaca
     6181 ttcttgcctt taagactgct caactttagt ttgccgccct tgctgctctc aagctcaaag
     6241 tcaggcgcct tatcgccgac ctcaagcgcc cgcgctgcag tattcgcgcc gacaacaaca
     6301 gctatggcaa acgccagaga aaacattatt tttgtggttg atttcatcgc ttcctccctt
     6361 ttgacgaaac gtcgggtcac tattccgaac gcgtaccgct ttatgtctta gtttatgggt
     6421 tactgtcaac cgcaacgagc ggacaattat actatgcgtt gctttgatga cgtgcttggc
     6481 tgccgaacgc gggatagaag cattagcctg tctcgctaat gtgctgtctt tattcaatgg
     6541 cgcggccatt gtctgcggtc agactgaatg gcggaagcaa ggaaa
//
LOCUS       DASUTG010000845         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1022185, whole genome shotgun sequence.
ACCESSION   DASUTG010000845 DASUTG010000000
VERSION     DASUTG010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022185"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VF089_19675"
     CDS             <1..1322
                     /locus_tag="VF089_19675"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX6176235.1"
                     /translation="LMKTGTARDLQKNVKAWVGGLRQHQTLVAGQGKPAAVSVAIEGS
                     DSREPMSIASPSATARDLRLDFFRGLALFCIFIDHIPNNILAQFTLQSLMFADAAEVF
                     ILISGYTAGMVYGRAMERQGFLIAAVRIYHRVWQLYVAHIFLFMIFMATMAYTAGALN
                     TSLYAEEFRAANFLNEPGLAVVKALTLQFQPAFMDILPLYIVLLAVLPLVVTGYRSWP
                     SVVIFASMSLWVAVQIDKRIALPAYPGPDQVWFFNPFAWQALFFLGAWFGWRASHGGV
                     SWLNDRWLFYLALGLSLAGFLIRFSWTLHDFYDPIPVFASGELLWPLLSKGDLGFLRF
                     ANVLALALLVARLIHPQARFLASGAARPFVVCGRNSLHIFCLGILLSVLGQLVLNEFF
                     GGILMQLAVSAAGIGIMIGVAAFMEWFDAAQHKSGGPVGATARGARG"
     gene            1319..2110
                     /locus_tag="VF089_19680"
     CDS             1319..2110
                     /locus_tag="VF089_19680"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6176236.1"
                     /translation="MTPAFLRYIFILITAASIGVAPVPAGAVDQAHECPMPERFYAFE
                     PPLTKTPKALATGREVVIVALGGSSTLGLAAGASALAWPARLGSVLNDKLPSARINVV
                     NRSVARQTAKQALERMDRDILPLKPTLVIWETGTMEAVRGADVDAFRETVRTGIDELR
                     AAGVEVVLMNMQFSRETDAMIHFEPYLIALRQVADANEVPLFRRRGIMRYWAESGLID
                     LRVKEDKKRRQLAVKLYDCIGRAMADFVTRGIPDGNSAASPGSGR"
BASE COUNT          515 a          819 c          811 g          634 t
ORIGIN      
        1 cacttatgaa aaccggcacg gcccgtgatt tacagaagaa tgtcaaagcg tgggtaggcg
       61 gtctccggca gcaccagact ctcgtcgcag gccaaggcaa accggccgcc gtgtctgtgg
      121 ctatcgaagg aagtgactct agagagccta tgagcatcgc gagtccttca gccacggcgc
      181 gagacctccg gcttgatttt tttcgcggcc tcgcgctttt ttgcatattc atcgatcata
      241 tcccgaacaa catcctggcg cagtttacgc tccagtcgct aatgttcgct gacgcggccg
      301 aagtgttcat cttgatctcc ggctacaccg ccggcatggt gtacggtcgc gccatggaac
      361 ggcagggctt tttgatagcc gcggtccgca tctaccacag agtatggcag ctctacgtcg
      421 ctcacatatt tctgttcatg attttcatgg cgacaatggc gtacaccgcc ggtgccctca
      481 acacctcgct ctacgccgag gaattccggg ccgccaactt cctcaacgaa ccaggcctag
      541 cggtagtgaa ggcgctgacg ctgcaattcc aaccggcatt catggacatc ctgccactct
      601 acatcgtcct gttggcggtc ctcccgctcg tggtgaccgg atatcgatca tggccgagcg
      661 tggtgatttt cgcctctatg tcgttatggg tcgcggttca gatcgataaa cggattgcgc
      721 tgccggcgta tcccggtccg gatcaagtat ggtttttcaa cccgttcgca tggcaggcgc
      781 tctttttctt gggggcctgg ttcggctggc gcgccagcca cggtggagtt tcctggctca
      841 acgatcgctg gctgttttat ctcgcgctgg gcctctcgct ggccggtttc ctaatccggt
      901 ttagctggac gctgcacgac ttctacgacc cgattccggt tttcgccagc ggcgagcttt
      961 tgtggccgct cttgagcaag ggagatttgg gcttcctccg tttcgccaat gtactcgccc
     1021 tcgcgctcct cgtcgcacgc ctaatccatc cgcaggcgcg gtttttggcc agtggcgccg
     1081 cgcggccctt cgttgtttgc gggcgaaact cccttcatat cttctgtctt ggcatcctgc
     1141 tctcagtgct ggggcaactg gtgctcaacg aatttttcgg cggtattctc atgcagcttg
     1201 cggtctccgc ggccggcatc gggatcatga tcggcgtggc tgcgttcatg gagtggttcg
     1261 atgccgcaca gcacaagtcg gggggtcccg tgggcgcgac ggcgagagga gctcgaggat
     1321 gacacctgcc tttctgcgct acatttttat tctgatcact gcggctagca tcggggttgc
     1381 accggtaccg gcaggcgccg tcgaccaggc gcacgagtgt cctatgcccg agcgcttcta
     1441 cgcctttgag ccgccgctga caaagacacc caaggcgctc gccaccggtc gggaggtggt
     1501 tattgtcgcg cttgggggct cgtcaaccct cggccttgcg gccggggcga gcgctctcgc
     1561 ctggcccgca cgtctgggtt cggtccttaa cgataaactt ccctctgcgc gcatcaatgt
     1621 tgtcaaccgc tccgtcgcac gacagacagc aaagcaggct ctagagcgca tggatcgaga
     1681 tattctgccc ctgaaaccca ctcttgtgat ctgggagacc ggcacgatgg aggcagttcg
     1741 tggcgccgac gtcgacgcat tccgggagac agtgcggacc ggaatcgacg aactgcgcgc
     1801 cgccggagtc gaggtagtgt tgatgaatat gcagttctcg cgtgaaaccg acgcgatgat
     1861 tcacttcgaa ccctacctca ttgcgttacg ccaagtggcg gacgccaatg aagtcccact
     1921 tttccgccgc cgtggcatta tgcgctattg ggccgaaagc ggcttgatcg atctgcgggt
     1981 caaagaggat aagaagcgcc gccagcttgc cgtcaagctt tacgactgca tcggtcgcgc
     2041 gatggccgac ttcgtgacgc gtggcatacc cgacggtaac tccgccgcta gtcccggcag
     2101 cggccgatga acgcgtctta atgcgctttc taagctgtga gttcatgcta ttggccacct
     2161 ttggcgatct cgcaccgcag tctgaactgc gctacagggg atcgcgcatg tcaaggtgag
     2221 gcctgcccaa cagcgtatgg attccttctt gatacaccgt ctcggtgatt taccgattcc
     2281 gcgttcggtc cgcgatgata ccgtcgaaag attaatcgtc tttcagccac agagcgaggc
     2341 tgcggctttc acgactggcc atgacacggt ttcaaaccag accgaatcga taccgagagt
     2401 ggcgtgaatt ccaattcgcc agcgcgcctg atctatcgca gcgaagatct ttggattggt
     2461 aaggccagag tatcggttgg tcaaggcagg ttagacgcga tgcttggctt cactgagccg
     2521 aagcccatcg aagcagaaat cgcgcgggcc cagatttccg gacaccgcgc cacagagttt
     2581 gggaatctga cagtaaaata gttgcaacaa cgacttagat tattaagcca ccgcagccac
     2641 tttgtcgtac atccgaacat caggagttcg tcgagccgga gacggcgcgg cggttacttc
     2701 tacaatcgag gcgggccctt ttccgttgcg ctgcacccag agatggcggg agtgaaaggg
     2761 acgtagggtg gcgcggtgg
//
LOCUS       DASUTG010000846         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_280083, whole genome shotgun sequence.
ACCESSION   DASUTG010000846 DASUTG010000000
VERSION     DASUTG010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280083"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VF089_19685"
     CDS             <1..795
                     /locus_tag="VF089_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6176237.1"
                     /translation="DGATNFGIIKGSDDSAVLIDADIRRMDEIDDALEKTGCKKVRYL
                     VNTHENFDHSSANDYFEKNGTVVVGTDGCYQALEEDGDEKFAEMAGRSPELWNRFPGL
                     KMANLQITFPQEMTLRLPGVAVRLLFAAHNGKSHSRGDAIAILEHDKILFAGDLLYTN
                     FHPVTVYGNIPNWIQSIKELLGHDFVSVVPGHGPVSSGGNDVYKSAFEKFRSYLEDFD
                     GKLAEMKRGKQSPSEVESYMKSGAYASMGKTWMVKRNIEYSLKELD"
     gene            798..1775
                     /locus_tag="VF089_19690"
     CDS             798..1775
                     /locus_tag="VF089_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176238.1"
                     /translation="MLRLLVSLLMIACLAGSSVSVYAADKLRVGFPSLATALSPTWVA
                     AKKGFWKKYDLDVELILLSGAVIIPTLLSGSLDVVIGSDTSAIISNLKGGNNLRLGVT
                     TNSLGSTLVTQRSVDSIQQLKGKVIGIGSQGFSSLELRLSKLLLDNGIDPEKEVKFLP
                     IGGNPSARVAALEKGLVIAAMITPPYDRVAIESGMRSLAKIDAPVIAGGINVRSDYLQ
                     GNREKLVRFLKGYIEGIHFLVSNKRETINIFSDYLGARDPNVIEFFYGEIAGRVEKNL
                     RPDPESVRFTLDFIGRLNPKAKEVKTTDFSDLSLLEEIHKSGFIEKLYR"
     gene            complement(1974..2972)
                     /locus_tag="VF089_19695"
     CDS             complement(1974..2972)
                     /locus_tag="VF089_19695"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176239.1"
                     /translation="MKQTSALVITILLVIVTLFTPIDLHAEKIRVGSGGFTPLHSMIW
                     AAHDQGVFEKYGFDVEYLALNSGTLGAQTLLSNEIQFLFSTGALAVTANLQGGDMTMV
                     SGGFNFFAFKLVARPEIRSAEDLKGKRFAISQFGSATDFAVQASLEKLGINPKQATLI
                     QLGGNPSRIAALTGGSADASVFSEPVATLAIKKYRMNLILDMAAAGLAFPQSTLMVKR
                     SYLEANRDKVTSFLKAMIEGLYIVKKDKALAIQLTKKYIRAEDEMYAVGYDYFLGQYG
                     EDLLNMPDRKGLELVIAQTAKTNPKAKGQTPESLRLMDASVLEEIKKSGFVDKLKR"
     gene            complement(3500..>3698)
                     /locus_tag="VF089_19700"
     CDS             complement(3500..>3698)
                     /locus_tag="VF089_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176240.1"
                     /translation="FMVKPRPVANEFGAFSTEWILDNEIRTKLLSPTAYTLRAEDENG
                     SVLAHAPFVFAAEKKEAKKSK"
BASE COUNT          915 a          923 c          968 g          892 t
ORIGIN      
        1 gacggggcta cgaattttgg cattatcaaa ggcagtgatg attcggcggt attgatcgat
       61 gccgacatcc ggcgtatgga cgagatcgat gacgcccttg aaaagacagg gtgtaaaaaa
      121 gttcgctatc tggtgaacac gcacgaaaac tttgatcaca gctccgcgaa tgactatttc
      181 gagaaaaacg gaactgtcgt ggtcgggacc gatggctgct atcaggcgtt ggaggaggac
      241 ggagatgaaa aattcgccga gatggcgggg cgatcgccgg agctttggaa ccgtttccct
      301 ggattgaaga tggccaactt gcagatcacg ttcccgcaag agatgacact tcgtcttccg
      361 ggcgtcgctg tacggctact ctttgcggca cataatggca agagccatag ccgcggagac
      421 gctatagcga ttctcgagca cgataagata ctcttcgcgg gcgacctact ttatacgaac
      481 tttcacccgg tcaccgtcta cggcaatatt cccaattgga tccagagcat caaagaactc
      541 cttggccatg attttgtttc cgtggtgccc ggacatgggc cggtatccag cgggggaaac
      601 gacgtctaca aatccgcctt cgagaagttt cgcagctatc tggaggattt tgacggcaag
      661 ctcgcagaaa tgaagcgagg gaaacaaagc cccagtgaag tcgaatcgta tatgaagagc
      721 ggcgcgtacg catccatggg taaaacctgg atggtcaaac ggaacatcga atattcactg
      781 aaagagctcg actaactatg ctgcgtctct tagtttccct gcttatgatc gcctgtcttg
      841 ccggctcatc cgtcagtgtt tatgccgccg acaagcttcg tgttggcttt ccctctctgg
      901 caacggcctt gagtcccacg tgggttgcag caaagaaagg cttttggaag aaatacgatc
      961 tcgacgtgga gctcatcctg ctatcgggcg cggtgattat tccgacatta ctcagcggaa
     1021 gtttggatgt cgtcatcggt tcagatacat cggcaattat ctcgaactta aaaggaggaa
     1081 ataatctgcg cctgggcgtg accaccaact cactgggttc cactctggtg acgcagcgaa
     1141 gtgtcgactc gatccagcaa ctaaaaggca aggtaatcgg catcggcagc caaggtttca
     1201 gttcgctcga gctgcgctta tcgaagctgc tgctagacaa cggcatcgat ccggagaaag
     1261 aagttaagtt tttgcccatc ggaggtaacc caagcgccag agtggcagct ctggagaagg
     1321 gactagtgat cgctgccatg atcacaccgc cctacgatag ggtcgcgata gagagcggca
     1381 tgaggtcctt agccaaaatt gacgctccgg ttatcgcagg cggaattaac gtcagaagcg
     1441 actatctgca aggaaataga gaaaaacttg ttcgtttttt gaaaggctac atcgaaggga
     1501 ttcatttcct ggttagcaat aaaagagaaa ccattaatat tttttccgat tatctcggtg
     1561 cccgcgatcc caacgtcata gaattctttt acggagaaat cgccgggcgg gtggaaaaga
     1621 acctgagacc tgatccggaa tcggtgcgct tcaccctcga cttcatcggc cgcctgaatc
     1681 caaaagcgaa ggaagtaaag accacggact tttcagatct gtcgctgttg gaagaaattc
     1741 acaaatcggg atttatcgag aaactgtatc ggtaaatacg agcgaaattc caagattaag
     1801 taactgatcg ggacccggcg tcatcgaaag gctggcgggc aggtttgcag caacactctt
     1861 gaagatcagc gacagcggct cgattcaact cgattcgcgc gaactgtcgc gctaccatcc
     1921 gtcctcatag ccatacacag cgtgagtcgg agttggcaat gatgcgttca tgatcacctc
     1981 ttcagcttat cgacgaaccc gctttttttg atttcttcga ggacgctcgc gtccatcaat
     2041 cggagcgatt cgggggtttg tccctttgct tttgggttgg tcttagccgt ttgcgcaatt
     2101 accagttcga ggcccttgcg atcgggcatg tttaaaagat cctctccgta ttgccccaaa
     2161 aaatagtcat agcccacagc atacatttcg tcttcggccc ggatgtattt tttcgtgagt
     2221 tgaatggcca acgccttatc cttcttgacg atataaagac cttcgatcat tgccttcaag
     2281 aacgatgtga ctttgtcacg gttcgcttcc aggtagcttc tctttaccat cagggtgctt
     2341 tgaggaaacg cgagaccagc cgcggccatg tcgaggatta gattcatgcg atatttttta
     2401 atcgccaacg tggcgaccgg ctcgctgaac accgacgcat cggcagatcc acccgtaagt
     2461 gctgctatgc gactggggtt gccgcctagt tgaatgaggg ttgcctgttt cggattaata
     2521 ccgagctttt ccaaactcgc ttgaacagca aaatcggtgg ccgagccgaa ctgactgatc
     2581 gcaaatcgtt ttcctttcag atcctccgcg ctcctgatct cggggcgagc gaccagcttg
     2641 aaagcaaaaa agttaaaccc gccggaaacc atggtcatat caccgccctg taaattggcg
     2701 gtaaccgcca aagcaccggt agagaaaaga aactggattt cattggagag tagggtttgt
     2761 gcgcccaacg tcccactgtt taacgccagg tattcgacgt cgaaaccgta tttttcaaac
     2821 actccttgat cgtgagccgc ccagatcatc gagtgaagag gtgtaaagcc gccgcttccc
     2881 acgcggattt tttctgcatg caaatcaata ggcgtgaaca acgtcactat cactaataga
     2941 atcgtaatca ccaaagccga tgtttgtttc acgtcacctc ctacaaagga ccgccgttaa
     3001 tgtttacccg caggaatgtt tatgcgacgg acacagtcct gataattagg ctaagagttc
     3061 ctggagtgga ctccattcag agccttttat caacttccgt tttcgtatta aactaaaagg
     3121 tcgtcttctt agctgggtgc cacccacgct cggccctgta tgacaccttc ggcgaggcca
     3181 gaggcgaaaa gaaggcccgg ctgttcgagg tgtacggata tactttccgg cagccaaact
     3241 cgaatatagg atgtgatttg tcttctctgc ggcgtctctg aatgttttca gaatgacaga
     3301 taacctatga gccgtatggg ttcgacagcg tcagtgaacc catacggcag tttttgccgg
     3361 gcagtatgtt aacggcaaac catgcggagg ggggcgcgcg gggaaagtct tgatccatca
     3421 ggcctgattg aagcctgacg cttgctgctg gaaacgaatt tatagtgagc tggtgaggca
     3481 ccgtcaaacg agaacggcgc tatttgctct tttttgcttc cttcttttcg gcagcaaaaa
     3541 caaacggggc gtgcgccagc acggacccgt tttcgtcctc ggcccgcagc gtataggcgg
     3601 tcggcgataa cagcttggtg cgaatctcgt tgtccagaat ccattcggtg gagaatgcgc
     3661 cgaactcatt ggcgaccggg cgcggcttga ccataaaa
//
LOCUS       DASUTG010000847         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1761952, whole genome shotgun sequence.
ACCESSION   DASUTG010000847 DASUTG010000000
VERSION     DASUTG010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761952"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VF089_19705"
     CDS             complement(<1..899)
                     /locus_tag="VF089_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176241.1"
                     /translation="MSKRFGRLSRREFLRTMISAGALVSTASLAVPRHAYSQSPSKNR
                     VTIAHGVGVYSLNPYAVTTSPIQAAWGSVMETLIDFDYEKRQYRGILAENWELDATRL
                     QFKLRKGIRFHDGTPLGSKDVAASLTRMLTDKESLVAPSLRNLKEIAAPDDLTVILTL
                     KKADANALEDLNNRVIMKQSVAEKMGEADNKPIGTGPFRFVSWQRSAEFVMGRSESYW
                     GPPAKIDQVIYKTIKEDAARIAALEAGQVDLISTIPPHEVERLKSNPRLTLQRVEGLR
                     PIFLVLSPAYKPLDNPKVRRAIT"
     gene            1193..>2176
                     /locus_tag="VF089_19710"
     CDS             1193..>2176
                     /locus_tag="VF089_19710"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX6176242.1"
                     /translation="MASIIESLVEYMIAARQSSLPAEVLQKGKNHLLDSLAAIVSGST
                     LKPGKLGLQHAREQGGEGQCSVLGSDFRTTPITAAFANGMAGHADETDDSNSQLHPGC
                     AIVPAALAMGELRGRDGESLLRAVILGYDIGFRFHQAFEPRSTSFGATFGCAAAAGAL
                     AELDALRLRYLVSYAAQQASGSRAWVGDDDHIEKAFDYAGMPARNGVTAALLVSSGFT
                     GNRDVLEGDQGIVKTYAPCDSGKLLKDLGQRFTITGCLVKKYPVGSPMMETVDATLAL
                     LSKHSVQASDIEKVIVRIPEGGARTVNNRHMPDVNVQYMVASILIDGKLTFE"
BASE COUNT          480 a          548 c          611 g          537 t
ORIGIN      
        1 tgggttatcg cccggcggac ttttgggttg tcgaggggct tatatgctgg agacaaaacg
       61 aggaagatcg ggcgtagacc ttcgacccgc tgcagagtta gccgaggatt ggactttaat
      121 cgctccactt cgtgaggagg aatagtgctg atcaaatcta cctggccggc ctccagcgct
      181 gcgattctag cagcatcctc cttgatcgtt ttgtagatca cttggtcgat ttttgcggga
      241 ggtccccagt agctttcgct acgtcccatg acaaattcgg cggatcgctg ccagctgacg
      301 aaacggaatg gaccagtgcc aatcggtttg ttgtccgctt cgcccatttt ctctgctacc
      361 gactgtttca tgattacccg gttgtttagg tcttcgagcg cgttggcatc agccttcttc
      421 aacgtaagaa tgaccgttag atcatccgga gctgcgatct ctttgaggtt tcgcaaggac
      481 ggcgcaacaa gactttcctt gtcggtaagc atccgcgtca gcgacgccgc aacatctttt
      541 gagcctaatg gcgtgccgtc gtgaaaccgg ataccttttc gcaatttgaa ctgtaagcgt
      601 gttgcatcca attcccagtt ttcggcgagg atgcctcgat actggcgctt ctcataatcg
      661 aagtcgatca gagtttccat caccgacccc caggcggctt gtattggcga ggttgtgacg
      721 gcgtagggat tcaaagaata tacgcctaca ccgtgagcaa tggtgacgcg gttttttgac
      781 ggcgattgtg agtatgcgtg tcttgggacg gcaagggacg cagttgagac tagcgcaccc
      841 gcggagatca tggttctgag aaattctctt cgactcaatc gtccaaacct tttgctcatc
      901 gcatcctcct cggctttcaa gccagattag gctagccagg tagaatcaaa taggaatgca
      961 aaccgcccgg ctccatttaa ttcggcaata cgattgaagt caattgtaca cagtggatga
     1021 gatcctttca caaactatcc gcatttgtca aaaagccttg acaccctctc cgaggccgaa
     1081 ctatatggat gacgtagttg gatcgaggcg ctctattttc attttagtcg taggtctcga
     1141 tgttcccctg ccttatccaa ctcccatctc aggagtaata acaggagggt taatggccag
     1201 cattatcgaa agccttgtcg agtatatgat tgctgcaaga cagtcgtcgt tgcctgcgga
     1261 ggtgctgcag aaaggtaaaa accatctgtt ggactcattg gctgcaatag tgtcaggctc
     1321 aactctaaag ccgggcaagc tcggcttgca gcatgcccgc gagcagggcg gagaaggcca
     1381 atgtagcgta ctcggatcag atttcagaac gacgcccatc acggctgcct ttgccaacgg
     1441 gatggcgggg catgcagacg aaacggatga ttcgaacagc caacttcatc ccggctgcgc
     1501 gattgtgccc gcggcgttgg cgatgggcga actaaggggc agggacggag aatctctgct
     1561 gcgtgcagtg attcttggct atgatatcgg gtttcgtttc caccaagcat ttgagccgag
     1621 atcgacgagt ttcggcgcta cattcggttg cgctgccgcg gcgggcgcat tggcggagtt
     1681 ggatgctttg cgcctgcgtt acttagtatc ttacgcggcg cagcaggctt cgggttcgag
     1741 agcgtgggtt ggcgacgatg atcacatcga aaaagcattt gactacgccg ggatgccggc
     1801 ccggaacggt gtcaccgctg ctctattggt aagctccggc ttcaccggca accgtgacgt
     1861 gctcgaaggt gatcagggca ttgttaaaac atacgccccg tgcgactcag ggaagctatt
     1921 gaaggatttg gggcagcgtt tcacgattac cgggtgcctg gttaaaaagt acccggtagg
     1981 ctctccgatg atggaaaccg ttgacgccac gttggcgcta ctttcaaagc attccgttca
     2041 ggccagcgat attgagaaag tgatcgtgcg gattccagag ggtggcgccc gcacggtcaa
     2101 caataggcat atgcctgatg tgaacgttca atatatggtt gcgtcgatcc tgatcgacgg
     2161 aaaactcacc ttcgag
//
LOCUS       DASUTG010000848         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2207999, whole genome shotgun sequence.
ACCESSION   DASUTG010000848 DASUTG010000000
VERSION     DASUTG010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207999"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VF089_19715"
     CDS             <1..323
                     /locus_tag="VF089_19715"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX6176243.1"
                     /translation="LPDRTRAHLGAGWNNFRPVGSLPKGASPYGVLDLAGNGWEWVSS
                     AYLPYPYNTADGREDLKRELVRVTRGGGQDSAAEELTTTHRGGHVSRNFHSGHHNIGF
                     RCAR"
     gene            354..998
                     /locus_tag="VF089_19720"
     CDS             354..998
                     /locus_tag="VF089_19720"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX6176244.1"
                     /translation="MAFYLFIKGAMMGFVVAVPVGPLGLLCINRALSLGPWYGLFSGL
                     GIATADALAGGIAAMSISLVSQFLVNHQFAFRLVGGLFLCYLGYEIYCTRPGQPLPRT
                     SRINGLMGTYLATFLLTLSNPVTILSFAAIYAAWHTQNITGHYGGAAVFAAGVFIGSA
                     SWWVALFLGLTLFRDRFNLRLLWWIHRISGGGIAVFGIVLLLSISPLSSILGMP"
     gene            complement(1076..2104)
                     /gene="egtD"
                     /locus_tag="VF089_19725"
     CDS             complement(1076..2104)
                     /gene="egtD"
                     /locus_tag="VF089_19725"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX6176245.1"
                     /translation="METEPLPSILAPDQKARWKTDQDGIWTLDGSRVDEIPSRSILTT
                     LWDQPRWLEAYHLYDQRGSELFEQICQLPEYYLTRTENSILEEKAARIIAAAPVECIV
                     ELGAGYSKKTIHLLTEQLRQRDKGIFAPIDVSESGLLASREAVARDFPKLEFHGLHAR
                     YEDGFTSIEQAVPTLFVFLGSTVGNFNHSDFPRFFRSLSQAMGPNDYLLLGADRIKDP
                     AVLEQAYNDSQGLTAQFILNVFCHINDLLGSNFDLGKMRYQARFNSEWQQIEMYAIAA
                     QRHRVSFPTVETSFDWQKDEKILVEISRKFDPQRLQEQLTFFDLLPIEHFTDPDEWFS
                     VLLLQKQG"
     gene            2162..>2671
                     /locus_tag="VF089_19730"
     CDS             2162..>2671
                     /locus_tag="VF089_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6176246.1"
                     /translation="MTDNRPTIEFDNVSFEIAKGKKLLVDLNLSIAQGETLVLLGRSG
                     SGKTTTMKMINRLLDPTDGEVRVEGISTQAWDPIILRRRIGYVIQEIGLFPHLTIEEN
                     IGLVPRLESWPADKIKSRARELLAMMSLEPDRFAGRYPAELSGGQRQRVGVARALAAD
                     PPMMLLDEPF"
BASE COUNT          583 a          684 c          756 g          648 t
ORIGIN      
        1 aactgccgga tcgcactcgg gcgcatttgg gcgccggatg gaataacttc cgtccggtgg
       61 gaagcttgcc gaagggagcg agcccctatg gcgtgcttga tctcgctggc aacggctggg
      121 agtgggtgag tagcgcgtat ctcccgtatc cctacaatac ggctgacggc cgtgaagatt
      181 tgaagcggga actcgtacgc gtcacccgag gaggcggtca ggattcggca gccgaagagt
      241 tgaccacgac ccatcgaggc ggtcatgttt cgagaaattt tcattcgggg catcacaata
      301 tcggttttcg ctgcgcgcgc tgaataacaa gtaaacttcc cgaccggcga gtaatggcat
      361 tttatctttt catcaaaggc gccatgatgg gatttgtcgt ggcagtacca gttggcccac
      421 ttggtctttt gtgcatcaat cgagcgctgt cgctgggtcc ctggtatggg ctcttttccg
      481 gcctgggtat cgctacggct gacgctttag ccggcggtat cgccgcaatg agcatttcgc
      541 tcgtatcgca gtttttagtt aatcatcagt ttgctttccg gctcgtcggc gggctctttc
      601 tttgttatct cggctacgaa atctactgca cgcgtcccgg gcagccgctg ccgcgaacga
      661 gcagaatcaa tggcctcatg ggaacgtacc tagcaacttt tctcctgacc ttgtcaaatc
      721 ccgtgaccat cctgtctttt gctgcgatct acgccgcctg gcacacccag aacataactg
      781 gccattatgg aggggcggcg gtattcgccg cgggcgtctt tatcggttcg gcatcgtggt
      841 gggtcgcatt gtttctcggc ctaacgttgt ttcgcgacag attcaatttg cgtctcttat
      901 ggtggatcca tcgcatctca ggcggcggca ttgccgtttt tggcatcgtt ttgttgctga
      961 gtatctcacc gctgagttcg atattgggaa tgccttgacc gagggtagac cggctttaaa
     1021 ggatgcacag cggcatgatg cggaacgggg caggttttgc gagatggaga ggatctcagc
     1081 cctgcttttg caaaagcaga acggagaacc actcatcagg atcggtgaag tgctcgatcg
     1141 gtagaagatc aaaaaacgtt agctgttcct gcaaccgttg cggatcgaac ttgcggctga
     1201 tctccaccag aattttctcg tctttctgcc aatcgaaaga tgtttctact gtagggaagc
     1261 ttacccgatg acgttgcgcc gctatggcgt acatttcgat ttgctgccat tccgaattga
     1321 agcgcgcttg atagcgcatt ttccccaggt caaaattgct cccgagcaga tcgttgatat
     1381 ggcaaaacac gttcagaatg aactgcgccg tcagcccttg tgaatcgttg taagcctgct
     1441 cgagaacagc cgggtctttg atacggtcgg cgccgagcag gaggtaatcg ttgggtccca
     1501 tcgcttgcga taatgagcga aagaaacgtg ggaagtctga gtggttgaag ttgccgacag
     1561 tgctgccgag aaaaacaaag agggtcggca cggcttgctc gatactggta aagccgtcct
     1621 catagcgcgc atgaagtccg tgaaattcta acttggggaa atcccgggca acggcttccc
     1681 gcgaagccag cagccctgat tcgctcacat cgatcggcgc aaagatgcct ttgtcgcgct
     1741 gccgcagctg ttccgtcagc aagtgaatcg tcttcttgct atatccggcg cctaactcga
     1801 cgatgcattc taccggcgcg gcggcgatga ttcgagcggc tttttcttcg agaatgctat
     1861 tttcagtacg agtaagataa tattccggca gctgacagat ctgttcgaaa agctcggagc
     1921 ctcgttgatc atataggtga taggcctcca accagcgggg ctgatcccac aatgtcgtca
     1981 aaatcgaacg ggacgggatt tcgtcaaccc gggagccgtc caaggtccag ataccatcct
     2041 gatcggtttt ccagcgggcc ttctgatccg gcgcaaggat cgatggcagc ggctctgttt
     2101 ccatatcaga acaggatata agaaacttga accggatgcg accgcaggag acgataggtc
     2161 aatgaccgac aatcgaccaa ctatcgaatt cgacaacgtc agctttgaga tcgccaaagg
     2221 gaaaaaactt ttggttgatc tcaacctttc gatagctcag ggggaaacgc tggtgcttct
     2281 cggccgtagc ggctcgggta agacaaccac catgaagatg atcaatcgct tacttgaccc
     2341 gactgacggt gaagttcggg tcgagggaat ctcaacccaa gcatgggatc ccatcatttt
     2401 acggcgacgg attggctatg ttattcaaga gattggcttg tttcctcatc ttacgattga
     2461 agagaatatc ggactcgtgc cgaggcttga aagctggcct gccgacaaga tcaaaagccg
     2521 cgctcgagag ctgctggcaa tgatgagttt agagcctgac agattcgccg ggcgttatcc
     2581 ggccgagctt tcgggtggac agcgccaaag ggtcggggtc gcacgcgcgt tggcggcaga
     2641 tccgccgatg atgcttctag atgaaccttt c
//
LOCUS       DASUTG010000849         9450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_280235, whole genome shotgun sequence.
ACCESSION   DASUTG010000849 DASUTG010000000
VERSION     DASUTG010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280235"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VF089_19735"
     CDS             <1..687
                     /locus_tag="VF089_19735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX6176247.1"
                     /translation="GGMVMVGAIYLENAPVDLTKVTPVARLTGEYEMIVVPAKSPHKT
                     MGDLVKTFKANPGGVSWGGGSAGGTDHILVGLIAKAAGVDPAKINYVPFKGGGEAIAA
                     IVGGHVTAGVSGIGEFAEHVKGGRMRALAVSSASPIDNVPTLKEQGLAVELTNWRGVF
                     GAPAITAAQRDALIKIVRGATESAAWKTTLEKLGWSPIFLSGEEYKKFIDEDTKRIGG
                     IIDSLGIKNK"
     gene            700..1200
                     /locus_tag="VF089_19740"
     CDS             700..1200
                     /locus_tag="VF089_19740"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6176248.1"
                     /translation="MKAKPAELVLSLAVLSLGIAVAIGTAGLSGAGGYARIGPNVAPA
                     VIAGGLILLGLWLLYEAVFGGWRNAVPDSPEARGEHDFSPSAFIWVSIGLIAQMLLIH
                     RAGFVLAQATLFTCVARGFGSRRYPRDFAIGLALGTAVFLFFVKFLNVNLPPGWLAPI
                     LQGAGI"
     gene            1207..2703
                     /locus_tag="VF089_19745"
     CDS             1207..2703
                     /locus_tag="VF089_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6176249.1"
                     /translation="MAETFSSLLQGFNVALTPLNLLWGFIGVMLGTFIGVLPGVGPAL
                     TVAMLMPLTVRLDPTGALIMFAGIYYGAMYGGSTTTILLNTPGESASIATALEGNLMA
                     KKGRAGPALATAAIGSFVAGTIATVLVTLLAPLIVEVALRFGPAEYFALMIFAFTTVS
                     AVIGSSTVRGLTSLFLGLLLGVIGIDSQTGKSRFAFGVPELLDGIDVVVLAVGLFAVG
                     EALYVAAYTSRLEEKLEQLKGSLWMSKEDWRRSAPAWLRATFIGFPFGSIPAGGAEIP
                     TFLSYATEKKLSKHPGEFGNGAIEGVAGPEAANNASVTGALVPLLTLGIPTSATAAIL
                     LSAFQNYGIQPGPLLFQTQPDLVWGLLASLYVGNVLLLILNLPLVGIWVKVLAIPRPL
                     LYGGILVFATLGAYSLHQSVVDLVILYIFGLLGFVMRRWNIPVAPAVIGLILGPLAET
                     QARRALAISQGDPMVFLIQPISAAILGISLLLLIVPVVVKVARRDRSL"
     gene            complement(2898..3182)
                     /locus_tag="VF089_19750"
     CDS             complement(2898..3182)
                     /locus_tag="VF089_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6176250.1"
                     /translation="MLSAIVLLTTERDKTKRVAEALADTEGVSEVYSVAGRFDLAAIV
                     RASDNDQLAEIVTEHIRALPGITSSETLIAFRVYSRHDLERMFSIGLEKA"
     gene            complement(3224..3949)
                     /locus_tag="VF089_19755"
     CDS             complement(3224..3949)
                     /locus_tag="VF089_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX6176251.1"
                     /translation="MKRDSIDGLVTPQCRILPRLTQRDSFLRALWLACLGVVLLFGDA
                     ATASSPHTVLFLGDSITVGYGLELSQAYPALIQQRIKKEGLNFKVINAGQSGDTSAGG
                     LARMDWLLKNKIDVLVLELGGNDGLRGLPVEVIRKNLQAIIDRARKQYPQMKIVVAGM
                     KMPPNMGRQYSREFEAMFAELAKKNNAALIPFILEGVGGVRQMNLADGIHPTASGHEI
                     VAENVWAVLAPVLRSIQAPVTRS"
     gene            3993..4703
                     /locus_tag="VF089_19760"
     CDS             3993..4703
                     /locus_tag="VF089_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176252.1"
                     /translation="MEKTILSVEHLNKLFKSGDKSVTILHDISFAIEEGSACAIVGPS
                     GSGKTTLIAICAGLERPTEGAVLLNGTAMHLASEEELSRVRNQLVGFVFQSFQLLPSL
                     TALENVMVPAEIRGENHARSRAEDLLRQVGLGARLHHYPIQLSGGEQQRVAIARAFMN
                     HPRILFADEPTGNLDAETAREIVRLIFQLNAQAGTTLVLVTHDRELTDQASRVITLKG
                     GRVLSDTREAGKSQVANA"
     gene            4720..7224
                     /locus_tag="VF089_19765"
     CDS             4720..7224
                     /locus_tag="VF089_19765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6176253.1"
                     /translation="MAFRDARRRIKPMLLAMSCVVLGVAAIVASFSFRDNLQTSVSAR
                     AKSLLGADLVIQSREPFSPEAEDLLKSIGGEQSRQIGFASMAFFPESGKSRLVEVRAI
                     RGNFPYYGALETEPMSARQDFQSGANAFVDETVMLQFNARIGDRLRIGEQEFRIAAKL
                     LKIPGETLAFSLLSPRIYVPLDHFDQAQFIQKGSRVRYRVFFKLPPEKNPDDIAQRLG
                     PELMRLRLETDTVTRRMTTIARFTENLSRYLQLAVFIAVLLAGVGVASAIHVYVKEKT
                     ASVAVLRCIGATPRETVYIYLVQAVLLALVGSILGAALGAGAQTLLPVVLKDFLPLDT
                     VISYSISGILAGVATGLGTTLLFALIPIVSLRKISPLFALRASYERDARFRDPVLWGI
                     YLIILLAIFAFALATTRQWLHGLSFTAAILAAFGLLTFISRSVSMLLKKFMPYFLPFA
                     WRQGLSSLHRPNNQTTAVMLSIGLGAFLLITLYSVRNMLVNQVAERGGRGDANLVLFD
                     VQKDQRQDLAALLRSFKLDSHEEVPIVTMRLAAIKNRPVDEIRADTTSKIPGWALRRE
                     YRSTYRSQLADTEKIIEGTWRGKVSPRTQPIPISLEKGIAENLEVSLGDELEFELQGV
                     SLPTEVASIREVDWQRIQPNFFAVFPEGVLETAPQFYAVVTRTDSTKVSADFQRAVVE
                     RFPNISMIDLTLVLNTLDSILGRVASAIRFVAMFTILTGIAVLISAVLSSRSQRLKEN
                     ILMKTLGAPRYQIITAIVAEYLFLGAISCVTAALLATLATWGLSFYFLGAIASVSWMP
                     ILIIGMIVTAATVLAGVIGCWGMFGRSTLEAFRLEA"
     gene            complement(7532..7915)
                     /locus_tag="VF089_19770"
     CDS             complement(7532..7915)
                     /locus_tag="VF089_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176254.1"
                     /translation="MKEKDTGAIFRIKEHKGCGIRAASYQIKPGKWVPEACFSVYTEA
                     GWRQLWLQSFAHCFSMSDHTYSNKIEADNWAFRMARTVIDKTLPEFDKHERPNAAKSP
                     LLTCFTKIWGIARRPFSTHDIHREH"
     gene            8548..9342
                     /locus_tag="VF089_19775"
     CDS             8548..9342
                     /locus_tag="VF089_19775"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176255.1"
                     /translation="MLSLRRISKVFGATDGESTAVPAVEAVSFEVRDGEFFSIIGPSG
                     CGKSTLLRIIGGLLRASEGELTVAGELVTGPHPSIAMVFQEESTFPWRTTLANVEFGL
                     QMRGVPEQRRRKTAVDMIGLVGLGGFEQRYPSELSGGMKQRVAIARALVLEPKILLMD
                     EPFGALDEQTRIILGEELLKIRDQLRQTIILVTHNINESVQLSDRVMVMTARPGQVKT
                     IVDIDLPHPRDSSIITSERFGRLVALVWSALREESIKSFKQSEQRH"
BASE COUNT         1958 a         2630 c         2630 g         2232 t
ORIGIN      
        1 ggcgggatgg tgatggtcgg cgccatatat cttgagaatg cgccagtgga tttgaccaag
       61 gtgacacccg tggcgcgcct taccggtgaa tatgaaatga ttgtcgtgcc ggcaaaatcg
      121 ccacacaaaa ctatgggcga tctagtcaaa acgtttaaag ctaatccagg cggcgtctct
      181 tggggcggcg gctccgccgg tggcactgat cacatactcg tcggtttgat cgccaaagcc
      241 gccggcgtcg acccggccaa aataaattac gtgccgttca aaggcggcgg cgaggcgatt
      301 gccgccatcg tcggcgggca cgtgactgcc ggcgtgtcag ggatcggtga atttgccgaa
      361 cacgtaaagg ggggccgcat gcgcgctctg gcagtctcga gcgcatcgcc gatagataac
      421 gtcccaacac tcaaggaaca gggcttggcc gtcgaactta ccaactggcg cggcgtcttc
      481 ggcgcgccag cgatcaccgc ggcccaacgc gatgctctga tcaagatcgt tcgcggtgcc
      541 accgaatcgg cagcgtggaa aacgaccctg gagaaactcg gctggtcgcc gattttctta
      601 agcggcgaag agtataaaaa attcatcgat gaggacacca aacgcatcgg tgggatcatc
      661 gattccctag gcatcaagaa caagtagccg gctcgaccca tgaaagccaa accggccgag
      721 ctggtgctat cgctggccgt cttgtcgttg gggattgcgg tggccattgg cactgcaggt
      781 ctgtcaggag cgggcggcta cgcgcggatc ggacccaacg ttgcccccgc cgtgattgcc
      841 gggggactta tccttttggg attgtggctg ctctatgagg ctgtctttgg cggctggcgt
      901 aatgccgtgc cggacagccc tgaggcgcgc ggcgagcacg acttttctcc ctccgctttc
      961 atctgggtga gcatcggcct gatcgcgcag atgctgttga ttcaccgcgc cggcttcgtg
     1021 ctggcgcagg cgactctgtt tacttgcgtt gcccgaggct tcggcagcag gcgatacccg
     1081 cgagatttcg ccattggcct ggcgctcggc acggcggtct ttctcttctt tgtcaaattt
     1141 ctaaacgtca acctgccgcc gggttggtta gcgccaatcc tgcagggcgc ggggatttag
     1201 cgtccaatgg ccgaaacctt cagctcactt cttcagggtt tcaatgtcgc actgacgccg
     1261 ctcaatctcc tatggggatt catcggggtg atgctcggga cgtttatcgg cgtgctgccg
     1321 ggagtcggcc ccgctctcac cgtcgcgatg ctcatgccgc tgaccgtcag actcgatccc
     1381 acgggcgcgc tgatcatgtt tgccggcatc tattacggcg ctatgtacgg cggatccacc
     1441 acgacgattt tactcaacac gcccggagaa tcggcctcca tcgccaccgc gctggaaggt
     1501 aacctgatgg cgaaaaaggg tcgtgccgga ccggcgcttg ccaccgctgc catcggctca
     1561 ttcgtcgccg gcaccatcgc gaccgtgctg gtgaccctgc tcgctccctt gattgtcgag
     1621 gtcgcgctca ggttcgggcc cgccgagtat ttcgcattga tgatctttgc gttcaccaca
     1681 gtatcggcgg tgatcggcag ttcgacagtg cgcggcttga ccagtttgtt tctcgggcta
     1741 ctgctgggtg tcatcggcat cgatagccag accggcaagt cccggtttgc cttcggcgta
     1801 cccgagttgc tcgacggtat cgacgtggtc gtacttgccg tgggtctgtt cgccgtgggc
     1861 gaagcgcttt acgttgcggc atataccagc cggttggaag aaaagctcga acagctgaaa
     1921 ggttcgctgt ggatgtccaa agaggattgg agaagatcgg cgccggcatg gctgcgggcg
     1981 acctttatcg gctttccgtt cggctctatc ccggcagggg gcgccgagat cccgacattt
     2041 ctatcttatg caaccgaaaa aaaactgagc aagcatccgg gggaattcgg caacggcgcc
     2101 atcgaaggcg tcgccggtcc cgaagccgca aacaacgcat cggtaaccgg ggcattggtg
     2161 ccgctgctca cattgggcat tccgacgtcg gccaccgccg caatcttgct cagcgcgttt
     2221 cagaactatg gcattcagcc cggtccgctg ctcttccaaa cccagccaga tctcgtgtgg
     2281 ggattgctcg ccagtctcta tgtcggcaat gtgcttttgc tgatcctcaa tcttccgctc
     2341 gtcggcattt gggtaaaggt gctggcgatt ccacggccgt tgctttatgg cggaatcttg
     2401 gttttcgcta ccctcggagc ttatagtttg caccagtcgg tagtcgatct ggtgattctt
     2461 tatatctttg ggttgctcgg tttcgtgatg cgccgctgga acattccggt tgcgccagcg
     2521 gtgatcggac tcatcctcgg cccgctcgct gaaactcagg cacggcgggc gcttgccatc
     2581 agccaaggtg atccgatggt atttctgatt cagcccattt cagcggcgat cttgggtatt
     2641 tctttgctgc tgctgattgt cccggtcgtt gtcaaggttg cgcggcgaga cagatcgctg
     2701 taaatccctc gccacacctt ccgttctggt aaattgctga agaacttaga tcagcttgcg
     2761 gaaaagtggt tctgtcaggc tgctcaaaag gtctcacagg cgaggcgcgc gaagttcgct
     2821 gagctgaggc gtacgcttca gtacgttgaa gcgagtcggg caagcgcaac gacacctatg
     2881 aggctttcgg cagcctgcta cgctttctcc agaccgatag aaaacatgcg ctccagatca
     2941 tgtcgtgaat aaacgcggaa ggcgatcagg gtctctgagc tagtgatacc cggcaatgcg
     3001 cggatatgtt cggtgacaat ctcagcgagc tgatcattgt ctgaagcacg gacgatagcg
     3061 gcaagatcaa agcggcccgc taccgagtaa acctcgctaa ctccctcggt gtcggcaagc
     3121 gcttccgcaa ctctcttggt tttgtctctt tctgtcgtta gcaaaacgat tgcggacagc
     3181 ataattggcc tccttgatga taactgcgcc ggaacgtctt gaattagctg cgagtcaccg
     3241 gcgcttgaat tgagcgcagc accggtgcca aaaccgccca aacgttctcc gcgacaattt
     3301 cgtgcccact ggcagtggga tgaataccgt cggcaagatt catctggcgc acgccgccga
     3361 cgccttctaa tatgaacggg atcagcgcgg cattattctt ctttgccagc tcggcaaaca
     3421 tcgcttcgaa ctcacggctg tactggcggc ccatattggg cggcatcttc atgcccgcaa
     3481 cgacgatctt catctgagga tactgttttc tagcgcgatc gatgatcgcc tggagattct
     3541 tgcggatgac ttccacaggt aaacctcgca agccgtcgtt gccgcccaat tcaagcacca
     3601 ggacatcgat tttgttcttc agcagccaat ccattctggc caagccgccg gcgctggtat
     3661 cgccgctttg acccgcgttg atgaccttga aatttaaccc ttctttcttg attcgttgct
     3721 gaattagcgc gggataggct tgcgacaatt caaggccgta accgacggtg atactgtcgc
     3781 cgaggaacaa aacggtgtgc ggagacgatg ctgtggcggc gtccccgaac agcagaacaa
     3841 cgccaaggca cgctagccat agcgccctca ggaacgagtc gcgttgtgtt aggcgcggta
     3901 aaattctaca ctgaggcgta actaatccat cgatgctgtc gcgtttcatc ctgccgttcc
     3961 ttacctcgac gacttaatac gaaaaatgac ccatggaaaa aactatctta agcgtagagc
     4021 atttgaacaa gctttttaag agcggtgata aatccgtaac cattcttcac gacatctcgt
     4081 ttgcaatcga agagggcagc gcctgcgcga tcgtgggtcc ctccggcagc ggcaagacta
     4141 cgttgatcgc catctgcgcc ggccttgaac ggcctactga aggagctgtg cttttaaacg
     4201 gcactgccat gcatttggcg agcgaggaag agctatcccg cgtacgcaac cagcttgtcg
     4261 gttttgtgtt ccaaagcttt cagctgctcc cttcgctcac cgcgctcgaa aatgtcatgg
     4321 tgccggctga aatccggggt gaaaaccacg cgcgcagccg cgccgaagat ctgctgcggc
     4381 aggttggtct gggcgcgcgg ctgcatcact atccgattca gctttccggc ggcgagcagc
     4441 agcgtgtggc cattgcccgt gcattcatga atcatccgag aattcttttt gccgacgaac
     4501 caaccggcaa tctcgacgcc gaaactgcca gggagatcgt gcggctcatt tttcaactca
     4561 acgctcaagc cggaacaact ctggtattgg tgacccatga ccgggagttg accgaccaag
     4621 ccagtcgggt catcaccctg aaaggcggca gagtactctc ggacacgcga gaggccggga
     4681 aatcccaggt cgccaatgct tagagcgtgg atttggaaga tggcttttcg ggatgcccgg
     4741 cgccgcatca aaccaatgtt gctggcgatg tcgtgcgtgg tcctgggagt cgccgcgatc
     4801 gtcgcatcat tttcattccg tgacaatctg cagaccagcg tgagtgcccg tgccaagagt
     4861 ttgctgggcg cagatctggt cattcaaagc cgcgagcctt tctcaccgga agcggaagac
     4921 ttgctgaaat cgatcggcgg cgagcagtcg cggcagatcg gttttgcatc gatggccttt
     4981 tttccagaga gcggcaaatc gcggctagtc gaagtccgcg cgattcgagg caatttccct
     5041 tattacggcg ctctggagac cgaaccgatg tcggcacgcc aggactttca aagcggcgcc
     5101 aatgctttcg tcgatgaaac ggttatgctg cagttcaacg cgcgcatcgg cgatcggctg
     5161 cgcatcggcg agcaggagtt tcgcatcgct gcgaagctgt taaaaattcc cggcgaaacg
     5221 ctggctttct cgctgctcag tccgcgtatc tatgtgccct tggatcattt tgatcaagcg
     5281 caatttattc aaaaaggtag ccgggttcgc tatcgggtgt tttttaaatt gcccccggaa
     5341 aagaatcccg acgatatcgc acaacgtctt ggtcccgaac tcatgcgcct gcgcctggaa
     5401 accgataccg taacccgccg gatgacgacg atagcgagat ttaccgagaa tctatcgcgc
     5461 tatttgcagc tcgcagtatt tatcgccgtt cttcttgccg gtgtcggtgt tgccagcgct
     5521 attcatgttt acgtcaagga gaagacagcc tcagtggccg tgctgcgctg cattggcgcg
     5581 acgcctaggg aaaccgtcta catttacctg gttcaagcag tgctgcttgc gctcgtcggc
     5641 tccattctcg gagcagctct cggcgccggc gcccaaaccc tgctgccggt agtccttaag
     5701 gattttttgc cgttggacac ggtgatctcg tacagcatca gcgggatttt ggctggcgta
     5761 gccaccggcc ttgggaccac gctcctgttt gccttgatcc cgattgtgtc actaagaaag
     5821 atctcgcccc tttttgcatt gcgcgcttcc tatgagaggg atgcccgctt tcgcgatccc
     5881 gttctctggg ggatttatct gatcattctt ctcgcgattt ttgcttttgc attggccaca
     5941 acccgccagt ggctgcacgg attgagcttc acggcagcga ttctcgccgc ttttggacta
     6001 ctcaccttca tctctcgcag cgtgtcgatg ctattgaaga agtttatgcc ttactttttg
     6061 ccgttcgcgt ggcgtcaagg cctttccagc ctgcaccggc ccaataacca aaccactgcc
     6121 gtcatgcttt ccatcggcct cggcgcattc ctgctgatta ccctttatag cgttcgcaac
     6181 atgttggtta accaagtcgc ggaacggggt ggccggggcg acgccaactt ggtgctgttc
     6241 gatgtacaaa aggatcaacg tcaggacctg gccgcgctgt tacgatcttt taaactcgat
     6301 agtcatgaag aagtgccgat tgtaaccatg cggctggctg ccatcaaaaa ccggccggtg
     6361 gacgagattc gcgccgacac aacctccaaa attcccggat gggcgctccg acgcgaatat
     6421 cgctcgacgt atcgaagtca gttggccgat acggaaaaga tcatcgaagg aacctggcgc
     6481 gggaaagttt caccgcgcac tcagccgata ccgatttctt tggaaaaagg catcgccgaa
     6541 aacctcgagg tgagcttggg ggatgaattg gaattcgagc tgcaaggcgt ttctttgcca
     6601 accgaagtcg cgagcattcg cgaagtcgat tggcaacgga tccaacccaa cttctttgcg
     6661 gttttccccg agggggtact ggaaaccgcg ccgcaattct atgcagtggt gactcgcacg
     6721 gattcaacta aagtttccgc cgacttccag cgggcggtgg tggaacgctt tccaaacatt
     6781 tcgatgattg atctgaccct ggttctcaac actctagatt ccattctggg tcgggtggca
     6841 agcgcgattc gcttcgtcgc aatgtttact attctgacag gtatcgcggt gttgatcagc
     6901 gcggtgttga gcagccgtag tcaacggctg aaagagaata tcttaatgaa aacactgggc
     6961 gcgccgcgat atcagattat cactgccatt gtcgccgagt acctgtttct gggggcgatc
     7021 tcgtgcgtca ccgccgcgct cctggcaacc ctggcgacgt ggggcctgag tttttatttt
     7081 cttggcgcca tcgctagcgt ctcgtggatg cctattctta tcatcggcat gatagttaca
     7141 gcggcaacgg tgcttgccgg cgtcatcgga tgctggggca tgtttggtcg ctcgaccttg
     7201 gaggctttta ggcttgaagc ttaatgtctg gttggtcccg ccgaagcggg tgtggcgagg
     7261 actgcggact gaacccaaat tccacagttc aaagtaacac agcgatcaag cggcaaaccc
     7321 tccggagcga aaacaatgga cctgtgcctt gtagcggcct gcaatctccg tgctgcagcg
     7381 gacctccggg gaaaattgcc accctgaagc aagcacctta gacggcattg aaatagttcg
     7441 cttctgtgtt ttcgctcccc caggcgcgcc gctcgaccgc tacacaaata atctctactg
     7501 gggaatccgg actgaatagc tgattgcggg ctcaatgttc cctatgaata tcgtgggtcg
     7561 aaaaagggcg gcgagcgatg ccccagattt tggtaaagca ggtgagcagg ggggacttag
     7621 ccgcattggg gcgttcatgc ttatcgaact cgggtaaggt tttatcgata accgttcgag
     7681 ccatgcggaa agcccaattg tcggcctcga tcttattgct gtaggtatga tcggacatcg
     7741 aaaagcaatg cgcaaagctc tgaagccaca gctgccgcca acccgcttcc gtatagactg
     7801 aaaagcaggc ctcaggtacc catttgccgg gtttgatctg atatgaagct gcccggatgc
     7861 cacagccctt atgttctttg atgcgaaaga tcgctcctgt atccttctct ttcattctct
     7921 ctggacgcat gggcgatctc tcctagcgat attcgattta ctgttccggc cagggcttag
     7981 aataaggtca gaattggcaa aagctatacc aggtatttct ggctcctggt ggcgagagaa
     8041 caggcaagaa ccgtcatcga agagcgcaaa aatgttcgtt tacgtatcgt gcaacgtctt
     8101 tttccacatc tttcctgtag ccataaacaa caagttaacc actataccag cttcatgggt
     8161 tctttccctt cctcgctgcc gacagtactg gccgggtgat cttctggcag gatgggcagc
     8221 tcttgtttga tgtagccaat ttatcgaccc gctatggaaa cggagattgc caatggagcc
     8281 tgaatagcta ctcggacagc attacgccgg gtccggcttc atattatatc gacgatgtca
     8341 gtatatcgcc gactcgtttg aatcacatcg ggctcgcagc cccgacagac ttgaagatct
     8401 tccaataacg aaatcccgca taccttccta tcctgctgtt ttgaagcttg ccgttttgga
     8461 tcggcaactc acaggttgtc gatccgccag atcgagggca aagcccttga cccccaacat
     8521 tgatctggga taactgcctc cttagggatg ctgagtcttc gcagaattag caaagtcttt
     8581 ggcgccaccg atggcgaatc gactgcagtg ccggcggtcg aagcggtatc tttcgaagtt
     8641 cgcgacggcg aattcttctc gatcatcggc ccatccggat gcggtaagtc gaccctcctg
     8701 agaatcatcg gcggattatt gcgcgcctcc gaaggcgaac taaccgtagc tggagaactc
     8761 gtcaccggac ctcatccgtc gatcgccatg gtttttcaag aggaatcgac ctttccctgg
     8821 cgaactacct tggccaacgt cgagttcggt ttgcaaatgc gcggggttcc cgagcaaaga
     8881 cgtcgcaaga cggccgtcga tatgattggt ctcgtaggcc ttggcggctt cgagcaacgc
     8941 tacccgtccg agctgtccgg cggcatgaag caacgtgtag ccattgcaag agcgctggtc
     9001 ttggaaccta agatcctact catggacgag ccgttcggtg cgctggacga acaaacgaga
     9061 atcattctgg gcgaggaact tctcaagatt cgtgatcagc tccgccagac catcatccta
     9121 gtgacccata acattaacga gtcggtgcag ctctctgatc gggtaatggt gatgactgcc
     9181 cgcccgggac aggttaaaac aatcgtcgac atcgacttgc ctcatcctcg ggattcctca
     9241 ataatcacct cggaaagatt tggccgtttg gtcgcgctgg tatggagcgc gcttagagag
     9301 gaatcgatca agagcttcaa acagtcagaa caacgacact gaggaggtca agtgatgaag
     9361 aagcgactgt tggtcgtaag acaatatgtc ttcttaggtt tattggtcct gctcattcta
     9421 ttcggagtca cttcttcttc atgggccgcc
//
LOCUS       DASUTG010000850         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2208050, whole genome shotgun sequence.
ACCESSION   DASUTG010000850 DASUTG010000000
VERSION     DASUTG010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208050"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF089_19780"
     CDS             complement(<1..763)
                     /locus_tag="VF089_19780"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176256.1"
                     /translation="MISKIVFWFLCLTNVSLLNAEQQARIPRVGYAASGGSINNPGVF
                     VESFRRGLRDLGYIEGKNILVEYRYPGTNPDRVSGFVEELIKLNVDVLVSSSAGAIGE
                     AKKATKTIPIVMVASFDPVEKGIIDSFARPGGNITGLARLTHELGGKRLELFKEAVPS
                     LSRLGVIAVRGSPSFREYETAARVLKISLESFEVNGSKPDLEGLFQAVVRARVNGLMP
                     VASRTLNPFTKQILQFAIKNGLPTMHEVGARVEAGG"
     gene            complement(816..1019)
                     /locus_tag="VF089_19785"
     CDS             complement(816..1019)
                     /locus_tag="VF089_19785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX6176257.1"
                     /translation="MGYGVNYGDLYRRAAIYVDKITKGAKPAELPVELPMRFELLINL
                     KAAKQIGVTIPPNVLARADRVIK"
     gene            complement(1003..1779)
                     /locus_tag="VF089_19790"
     CDS             complement(1003..1779)
                     /locus_tag="VF089_19790"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176258.1"
                     /translation="MIRKILWLLATFLLAHIQLAAAQHPVKNARIGFLFFGSEDQPHL
                     ESFHQGLGDLGYVNGKNIFIEYRYAERSSDRLAILAAELVALNLDVILTTTPAATRAV
                     QANSRTPIVGIGFDPVATGLVKSLAYPGGIVTGLSSSAGPEMMGKRLDLLKEAFPKIT
                     TVAMLWNPDAGQLAAQVLEGAKKGAKALGLQLRPHEVRSTGDIEGAANVLKQLQPDAL
                     VIPGGALMILNSKRLVDIATKLRLSAMYQNRRRRTDGLWS"
     gene            complement(2142..>2566)
                     /locus_tag="VF089_19795"
     CDS             complement(2142..>2566)
                     /locus_tag="VF089_19795"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX6176259.1"
                     /translation="KGRYMWRDLERICEAQRLPFRRPSRFPRNGLLAARIVCRFSQET
                     WIPTFVRAVYHANFAEDEDIADRGVVANCLKLVNQDAENVLDVAGSAETKILLREQTE
                     RAVDLGIFGAPSFIVDQELFWGNDRLEQALMWYQRRSG"
BASE COUNT          583 a          717 c          654 g          612 t
ORIGIN      
        1 gtcctccggc ctctacgcga gccccgactt catgcatagt cggcagtccg tttttgatgg
       61 caaattgtaa gatctgtttc gtgaaagggt tcagtgtgcg acttgcaacc ggcataagcc
      121 cgttcactcg tgccctcacg acagcttgaa ataaaccctc cagatcgggt ttagaaccgt
      181 ttacctcgaa ggattcgagc gaaatcttca gcacgcgtgc cgcagtctca tactctcgga
      241 agctcggtga cccgcggacc gcaatcactc ctaaacgcga caggctcgga accgcctcct
      301 tgaacaattc gagtcgtttt ccacctagct catgggtaag tctggcgagt cctgtgatgt
      361 ttcctccggg gcgtgcaaaa ctatcgatga tccctttctc caccggatca aaagaagcca
      421 ccataactat ggggatcgtt ttggttgctt tcttggcttc accgattgct cctgcactcg
      481 aactaaccag cacatcgaca ttgagcttta tgagttcctc gacgaagccc gagacccgat
      541 ctgggttcgt cccgggatag cgatattcaa ccaggatgtt ttttccttct atgtagccta
      601 aatctctgag tccgcgccgg aaggactcga cgaagacgcc gggattgttg atgctgccac
      661 cgctcgccgc atatccaact cgtggtattc tcgcttgctg ctcggcgttg agaagagaaa
      721 cattggtcag acaaaggaac cagaaaacaa tcttgctaat cattgcttcc gttccatttt
      781 aactggacag tgcttcccat tacgctgttc gactactatt tgatcactct gtccgccctc
      841 gccagcacat tcggcggaat cgtcacgccg atctgcttgg ccgctttcag attaatcagc
      901 agttcgaatc tcatgggaag ttccacgggt aactctgcag gcttggcccc tttggtgatc
      961 ttgtcgacat aaatcgcggc gcgacggtag aggtcgccgt aattaactcc atagcccatc
     1021 agtccgccgt cttcgatttt gatacatcgc cgacaagcga agcttcgtgg cgatatcgac
     1081 cagccgtttt gaattaagaa tcatcagtgc gccccccggt ataactagag cgtccggctg
     1141 aagttgcttt aagacgttcg cggcgccttc gatatctcct gtgcttctga cctcatgcgg
     1201 acggagttgt agtccaagag ccttggcgcc cttttttgct ccttcgagaa cctgcgctgc
     1261 aagctggccg gcgtcgggat tccacagcat cgcgactgtg gtgattttcg ggaacgcctc
     1321 cttgagcaga tccaagcgct tgcccatcat ttcagggccg gcgctgcttg ataggccggt
     1381 gacgattcca cccgggtacg ccaggctctt gaccaaacca gtagcaaccg gatcaaatcc
     1441 gatgcctacg ataggggttc ggctatttgc ctgaacagcg cgagtggcgg caggcgtagt
     1501 tgtaagaata acgtcaagat tgagcgcgac gagttcggcg gcaagtatcg ccagtctgtc
     1561 agaactccgc tcggcataac ggtactcgat gaaaatgttc ttgccgttaa cgtatcctaa
     1621 atctcctagt ccctgatgaa acgattccag atgaggttga tcctccgacc caaagaaaag
     1681 aaatcctatc cgagcattct ttaccggatg ctgcgccgcg gcaagctgaa tgtgcgcgag
     1741 aaggaaagtg gcgagcagcc agaggatctt tctgatcatg gccattgctc ctaccgcaac
     1801 ggatatgcca caaaacgaac tctatgaaaa tgataattta gaagccggga aagcgaaaag
     1861 aggtggccgt ttgactacag gggagtatca gatttgctgc gccataattt ctgcacaagc
     1921 aagatcactg aaagccgatg gagaggtgca cggtaggaaa cgtgttcgcg actatggttc
     1981 atgccttggt ccgatccgta gccgcccgta ttgcagattc actttcacgg aggtctctct
     2041 caacagccaa gacgcacgtt cggtaccatt ggcatggtac cggagcgcgt aaaagcttac
     2101 ccctctagcc atgcagatag gtgtcactgc cctgacaata cctacccgct tcggcgttga
     2161 taccacatca aagcctgttc tagccggtca tttccccaga acagttcctg gtcgacaata
     2221 aagctaggcg caccgaagat gcctagatcc accgcccgct ccgtttgctc tctgagcaga
     2281 atctttgtct cagccgaacc cgcaacgtcg aggacatttt ctgcatcctg gttcaccaat
     2341 ttcaagcaat tagcgaccac gccgcgatcg gcgatgtctt cgtcttcggc gaagttggcg
     2401 tgatagaccg cgcgaacaaa ggttgggatc caggtttctt gtgagaaacg gcagacaatt
     2461 cgcgcggcga gtagtccgtt gcgcgggaac cgcgatggac ggcggaatgg caggcgctgc
     2521 gcttcgcaga tacgttccag atcacgccac atgtagcgcc cctttt
//
LOCUS       DASUTG010000851         9893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_280242, whole genome shotgun sequence.
ACCESSION   DASUTG010000851 DASUTG010000000
VERSION     DASUTG010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280242"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1214)
                     /locus_tag="VF089_19800"
     CDS             complement(192..1214)
                     /locus_tag="VF089_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6176260.1"
                     /translation="MNAAHAGATLAFLLAVAPASAQSVKITPVGSHPGELCANDRAIV
                     FEDPTGVRLLYDSAHNVTGGDDPRLGTIHIVLLTHMHGDHVGDQKLKAPGAGTCANSE
                     RVAAPNSTTAEVVAAKNAALVMTRAMSGFVGNKVQGMRGGKPIGFCPQTDGATTVPVE
                     TVCSSQTDLGGVFVAKTANAAQGVEIAIVYASHVNNAPPALLSESQRAAAKADGAILE
                     YGPATGFVVKFTNGLTAYLSGDTGIHSEMKTVVNEYHKANLAVLNLGSNPGIFFSGAH
                     AMNELVRPASVILTHPNEPVTEGGKLRPASRTAALMKQLKPAAHIAISGRTMEFDGAG
                     KCVAGC"
     gene            complement(1571..2089)
                     /locus_tag="VF089_19805"
     CDS             complement(1571..2089)
                     /locus_tag="VF089_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018294438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEX6176261.1"
                     /translation="MNASIVRESAVASTVKDPGMRVPTGVRSIALCAVLLFANPAAIA
                     FSQSSCPGIHVKILSIRNSTGAVACALFESAAGFPTEFLHSATNIMMIKIKDAQARCD
                     FEDIPPGRYALAVIHDENMNGKLDTNWLGAPTEGYGFSNDAKAFLSAPSFSAASFLYD
                     GRNVDMTISLNY"
     gene            complement(2301..2990)
                     /locus_tag="VF089_19810"
     CDS             complement(2301..2990)
                     /locus_tag="VF089_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEX6176262.1"
                     /translation="MRHAERHRTQRIGWLRAAVLGANDGIVSTASLVLGVAAAGVDSQ
                     GILVAGVAGLVAGAMSMAAGEYVSVSSQADTERADLARESKELAVSPEQEQAELTTIY
                     MKRGLDPALASTVASLLMKHDALGAHARDELGISETVAARPIQAALASAGTFSLGAVL
                     PLLVVMLFPASALMWTVSGSSLVFLAMLGLLSAQAGGARVIVAASRVTLWGALAMALT
                     AGVGALFGVAT"
     gene            complement(3097..3555)
                     /locus_tag="VF089_19815"
     CDS             complement(3097..3555)
                     /locus_tag="VF089_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX6176263.1"
                     /translation="MKHVKRIVRWSIAALAITLVVIQFIPVDRTNPPVETEVRATAQV
                     REVLRRACYDCHSNETIWPWYSRIAPVSWLVARDVREGREELNFSTWNRLTTKERINA
                     LHESWETVEEGEMPLWFYIPTHPQARLSVQDRSVLRAWSLSTGSAGSEER"
     gene            complement(4429..4926)
                     /locus_tag="VF089_19820"
     CDS             complement(4429..4926)
                     /locus_tag="VF089_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX6176264.1"
                     /translation="MAPLTKQKQIVGLVGWLIVSFIAAAIGGAASIQAGPFYTQLVRP
                     DWAPPPSIFGPVWTVLYALMGIAAWLVWRVGGFRAAKSALTLFLVQLALNALWSWLFF
                     RWHRGDLAFADILLLWALIVVTLIAFWRVRPLAGALLVPYLLWVSFASALNYSVWQLN
                     PHVLG"
     gene            complement(4997..5818)
                     /locus_tag="VF089_19825"
     CDS             complement(4997..5818)
                     /locus_tag="VF089_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176265.1"
                     /translation="MAAAEVNGVRLFYELQGTGAVPLVLVHGSWDSHHDWDLVVPRLA
                     EAFRVLTYDRRGHSQSERPTGQGSVREDVADLAALLEHLGLVPAWVVANSFGASITLR
                     LACEHAELFRGLIAHEPPLFSLLGNDPGLAPLFSDVQRTIRAVVERIASGDHTGATEQ
                     FVETVALGPGTWATIPPNIQQAMIENAPTFLDEANDPEALAFELEWISDFSKPTLLTL
                     GDQSPPTFAPVVTRLSRALPHAEIVTLPGAGHVPHETHPDDYVEAVIKFTRKHTA"
     gene            complement(5965..6486)
                     /locus_tag="VF089_19830"
     CDS             complement(5965..6486)
                     /locus_tag="VF089_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6176266.1"
                     /translation="MKPHFAMMAQYNAWANARLYAEAWALSDESYRKDVGAFFRSLHG
                     TLNHLLVTDRIWMRRFTGEGEHPSKLNAIMFDDLPSLKAARRAEDARIVQYVEGLSEE
                     EIGRDFSYWTTQGVPQLFILRDLLAHWFNHQTHHRGQAHCILTVLGVPEPKPLDLLAM
                     YRKVPSVHQATSA"
     gene            complement(6607..7035)
                     /locus_tag="VF089_19835"
                     /pseudo
     CDS             complement(6607..7035)
                     /locus_tag="VF089_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
     gene            complement(7140..7505)
                     /locus_tag="VF089_19840"
     CDS             complement(7140..7505)
                     /locus_tag="VF089_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176267.1"
                     /translation="MGRKVDGLAGLIQEIKEGNQGKPLIRLSLLKPNDNDTSIWVGSL
                     VRPEAGQIKPGHIIRVLGYLSSIESTDSVVAKTVRDPLFLLGFCLVNQSTTTGLFLPS
                     AINQCDEWKNGTIPKNVRQ"
     gene            complement(8092..8661)
                     /locus_tag="VF089_19845"
     CDS             complement(8092..8661)
                     /locus_tag="VF089_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176268.1"
                     /translation="MFDRDRFIAECQHASAAAGSAGVLEAVARAVAEPGEILRALGEP
                     RRAGLHPLFRTAELTILNVVWGPRMSQLPHNHKMWAVIGIYTGREDNIFWRRMPERGD
                     GRIEAAGAKALSERDAVPLGHDVIHSVLNPVSRLTGAVHVYGGDFFALPRSEWDPERL
                     IEQPFDLQKALALFEESNRLHPVPSHEPA"
     gene            complement(8771..9355)
                     /locus_tag="VF089_19850"
     CDS             complement(8771..9355)
                     /locus_tag="VF089_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX6176269.1"
                     /translation="MGKNRFEAFSDGVIAIIITIMVLEMKVPHGEGIETLVPLIPVFL
                     SYVLSFVYLGIYWNNHHHMLHATQKVTGAMLWANLHLLFWLSLIPFTTGWMGENHFAA
                     APSALYGVVLLMAAVAYWILQQLIIASQGPDSILKKAIGGDWKGKLSPLLYVAAIPLA
                     FWWQWISLGLYVFVALLWLIPDRRIENVLTVADT"
     gene            complement(9373..9639)
                     /locus_tag="VF089_19855"
     CDS             complement(9373..9639)
                     /locus_tag="VF089_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176270.1"
                     /translation="MRTAIIIAVGFVLWAACLGVAKLLTSSGTSSMITATVAFLVIWF
                     IAAAANMWIGVSQAGYSFREELPIFLLILLLPSAVAIFVKWKFL"
BASE COUNT         2279 a         2864 c         2681 g         2069 t
ORIGIN      
        1 aacaagttca gccgatcatc ctcatactcg ttatcgttgt ggagaactgt cggcccgaag
       61 aaatgaacga tgatgttccg ccggaacccg cgcttcgaat ccatgaagcc acccccggag
      121 ccttctcatt ttcaaaacgg cttgcgcaac acgcctgtca taactccttg aaacccggtc
      181 gatgtcgcgg ttcagcatcc agcgacgcac ttccctgcgc cgtcgaactc catcgtgcgt
      241 ccgctgatgg cgatgtgagc ggccggcttg agctgtttca tgagcgctgc cgtgcgcgac
      301 gccggccgga gcttcccgcc ttccgtaacg ggctcgttcg gatgcgtgag gatcaccgat
      361 gcgggccgca ccagctcatt catcgcatgc gcgccggaaa agaagattcc cgggttcgac
      421 ccgagattca gcacggcgag gtttgccttg tgatattcgt tcacgaccgt cttcatctcg
      481 gaatgaatgc cggtgtcgcc cgagaggtag gcggtcagtc cattggtgaa cttcacgacg
      541 aagcccgtgg cggggccgta ttcgaggatg gcgccgtccg ccttcgcggc tgcgcgttgc
      601 gactccgaga gcagggcagg cggggcattg ttcacgtgcg aagcgtacac gatcgcaatt
      661 tccacgccct gcgctgcatt ggccgtcttc gcgacgaaaa cgccgccgag gtccgtttgc
      721 gagctgcaca ccgtctcgac cggcacggtg gtggcgccat cggtttgcgg acagaagcct
      781 atcggcttgc ccccacgcat gccctgaacc ttgttgccca cgaacccgct catggcgcgt
      841 gtcatcacga gcgccgcgtt cttcgccgcg acgacctcgg cggtcgtgga attcggcgca
      901 gccacccgtt ccgagttcgc gcacgtcccg gcacccggcg ccttgagctt ctggtcgccg
      961 acgtgatcgc cgtgcatgtg agtgagcagg acgatatgga tcgtgcccag ccgcggatcg
     1021 tcgccaccgg tcacgttgtg cgccgagtca tacagtaatc gcacgcccgt cggatcctca
     1081 aaaacgatcg cgcgatcgtt cgcgcacagc tcgcccgggt gcgagccgac cggagtgatt
     1141 ttcacgctct gagcgctggc cggagctacg gccaaaagga atgcgagggt tgcacctgcg
     1201 tgtgcagcat tcatgttttc ctccactggt ctggtgattc ggacgagatc attcgataga
     1261 cgtacactcc aagagctagg ttgcaatcta ccgctccacg aagaaaagaa cgagaaccac
     1321 aaccagccgc ccaaaggccg cggacaaaac agcagcgtac tcgatgccgc aaggcggata
     1381 aaggcccagg tcgggggccg gaaaagaagc atggcgccgg aaattggatc taccatgacc
     1441 tagtcgccca acgtctcgct cagttgcgct gtttctgcgt tggctgcagc gccttgttag
     1501 gcgcagcaac cccgaatctg tgtgctggat tgaagaaagt agaaactttg cgtagtcata
     1561 caaggagcac tcagtaattc aagctgatcg tcatatctac gtttcgcccg tcgtacaaaa
     1621 aactggcagc gctgaacgaa ggagcgctca agaaggcttt tgcatcgttt gagaaaccgt
     1681 agccctccgt tggggccccc agccaattag tgtcaagctt gccgttcata ttctcatcat
     1741 gaatgacggc aagcgcatac cttcccggcg gaatgtcctc aaagtcacag cgcgcctgcg
     1801 catccttgat ttttatcatc atgatattgg tggcggagtg cagaaactcg gtagggaagc
     1861 cggctgccga ttcgaaaaga gcgcaagcta cggccccagt gctgtttcta atgctgagaa
     1921 tcttcacgtg aattccgggg catgatgact gtgaaaatgc gatggcggcc ggattcgcaa
     1981 acaacaacac tgcacacaat gctatgcttc gaactcccgt ggggacccgc atgcccggat
     2041 ccttgaccgt ggaggccaca gcggactccc gaacgattga cgcattcatg attttcctct
     2101 cagctgccgc ataaccggaa tggatggcag cggaacccag gattacttca tcggatcaaa
     2161 cgcctaacgc ctaaagttca actgcgcaca cgaccagcgc agccatatcc gaactacgat
     2221 acgagttgct accagcaacc ttctatctca ttgtccgcgc gtcagttgca acgctcacgt
     2281 taggcggatc accgggagag tcaggttgcc accccaaaga gcgcgcccac ccccgctgtc
     2341 aatgccattg ccaaggcgcc ccacaaagtg acgcgtgagg cggcgacaat cacccgggcg
     2401 ccacctgctt gggcggacag caatcccaac attgcgagaa acaccagcga actgcccgag
     2461 acagtccaca taagcgcgga agccggaaac agcattacga ccagaagcgg aagaacggcg
     2521 cccagcgaaa acgtgccagc ggaagcaagc gcagcctgaa tggggcgagc cgctacggtt
     2581 tcggagatac ccagctcatc acgcgcatgt gcaccaagtg catcgtgctt cataagaagg
     2641 ctagccacgg tggaggcgag tgccggatcg agcccgcgct tcatgtagat cgtcgtaagc
     2701 tccgcttgct cctgttcggg gctgacggcc agttctttac tctctcgggc cagatcggcg
     2761 cgctcggtat ctgcctgtga actgaccgac acgtactcgc cggcagccat cgacatagct
     2821 ccagcgacga ggccagcaac gcccgcgacc aagatgccct gagaatcaac ccccgctgcc
     2881 gcaacgccta aaacgagact ggcggtggag acgattccgt catttgcacc aagcacagcc
     2941 gcgcgaagcc agccaatccg ttgtgttctg tgtctttcgg cgtgcctcat gaggcgctcc
     3001 ttgtagcaac atgtgcctga cgacgtttaa cgtgcagcat cagcggcagc acggaccgcc
     3061 ttccgccgca ctattgtatg ggcggtcacc acgtactcat cgctcctcag agcccgcgct
     3121 gcccgtagac agcgaccaag cccgcagaac cgacctatcc tgcacagaga gacgcgcctg
     3181 cggatgagtt ggaatataga accataacgg catctctccc tcctcgactg tctcccaact
     3241 ctcgtgcaag gcgttgatcc gctccttggt ggtcagccga ttccaggtcg agaagttcaa
     3301 ctcctcgcgg ccctcacgga cgtcgcgggc gaccagccat gacaccggtg cgatccggct
     3361 gtaccaaggc cagatagtct cattggagtg acaatcgtag catgcacggc gtagcacctc
     3421 tcgcacctgg gcggtggcgc gcacctctgt ctcgactgga gggttcgttc gatcgactgg
     3481 aatgaactga atcaccacca gggtgatggc tagcgcggca attgaccatc taacaatgcg
     3541 cttgacgtgt ttcatagcgc tcgctcgtcc tctactagag ttgttcgcct aacgagtcag
     3601 gctgagagga cgagatttcc gagcggcgcg agcggtcgaa gatccttctc cagccgattg
     3661 ttaggcgtct tactctagcc tgataattgt ttcattgatc ttgtatagct ccgattgaaa
     3721 gattgacaac ttgcagacag acagaataag ctcctgctcg attgagtcaa attctcgatg
     3781 cgataatcga ggatcaagga ctacttcatc aagcactgct tcgtgatcga aagtcattgc
     3841 gtctgattct tctcgttagg catctcacca tgtcgtggtt cgcctgtggc ataaaacaaa
     3901 ttagcccgac tctgactcat ccagtcgggc taagtaaacc aatcgaactc gcgactgatg
     3961 aaaaaccagt ctagggccgg ctaatgattg aggccttacc tgtgttacag caacatgcat
     4021 tccacaaaca gctgaccctt tattctggag tcggtcggcc gatttcatga agtttttgga
     4081 gatgaggtga ccggaaaagt atttcaagat aggaaaggct ctgcgcattt tcttatttta
     4141 gcgaaataca ttcgcatgct cgcgagctag acgtctatct tttcgagaag acttctctca
     4201 cgtagacaaa gcctggtgtt ttactgagga tcttcttgct ccagcttttg gaatccgccg
     4261 aatcatcctc aggatttttt ccttagattt gatcgacaac gcatcgtcgg atacggcgta
     4321 aaactggttt gttgagaatt cacatcagct caattacaca gcgtaactct tgaattaagc
     4381 cgagccgcga agcggcttcg gcttgaatga tttattaggc aaactgcatt accccaagac
     4441 gtgcggattg agttgccaca cagagtagtt gagtgcagag gcgaagctga cccacagcag
     4501 atacggaacg agcaacgcgc ctgctaatgg tctgactcgc cagaaggcga ttagggttac
     4561 gacgatcaac gcccacagta acaggatgtc agcgaaggct aagtcaccac gatgccagcg
     4621 gaagaacaac cagctccata gtgcattgag cgcgagttgc acaagaaaca aagtgagcgc
     4681 ggacttggct gcacgaaacc cgccaacacg ccaaaccagc caagcagcga tacccatcag
     4741 ggcatacaga actgtccaaa ccggcccaaa gatcgagggt ggaggtgccc aatcaggacg
     4801 gacgagctgg gtatagaacg gaccggcctg aatagatgca gctccaccaa tggctgcagc
     4861 gatgaagctg acaatgagcc agcctacaag accaacaatc tgcttctgct ttgtaagtgg
     4921 cgccatacgg aactccgatt gttgtctaac gcccggcatc acccgcgcgc aaagcgcgtc
     4981 gggcggcagc cgtgtgttat gcggtgtgct tacgcgtgaa cttgatgacc gcctcgacat
     5041 agtcatccgg atgtgtttcg tgcggaacgt ggccagcccc tggcaatgtg acaatctccg
     5101 catgaggaag cgcccgcgag agtctggtga ccacgggcgc gaaagttggc gggctctggt
     5161 cgccaagcgt cagcaaggtc ggtttcgaga aatcgctgat ccattcgagc tcgaaagcga
     5221 gcgcctctgg atcgttcgcc tcgtcgagga aggttggtgc gttctcgatc attgcctgct
     5281 gaatgttagg cggaatcgtt gcccaagtgc cgggtccaag cgctacggtc tcgacgaatt
     5341 gttcggtagc gccggtgtgg tctccggagg cgatccgctc aactacggct ctgatcgttc
     5401 tttgaacatc cgaaaaaagt ggggccaggc ctggatcatt gccgagtaac gagaacaacg
     5461 gtggctcgtg cgcaatgagg cctcggaaga gttcggcatg ctcgcaggcc aggcgaagcg
     5521 taatcgaagc gccgaaagag ttcgcgacca cccacgccgg cacgagcccc aggtgctcga
     5581 gcagggctgc gaggtcggcg acatcttcgc gcacgctgcc ctgtccggtg gggcgctcgc
     5641 tttggctgtg tccgcggcgg tcataggtca ggacccgaaa cgcctcagcc aaacgaggca
     5701 cgacgaggtc ccagtcgtgg tgggagtccc atgacccgtg caccaagacg agcggcactg
     5761 ccccggtgcc ctggagctcg tagaagagcc tgacgccatt cacctctgcc gctgccatgt
     5821 gctcacttct ccttggattc taactgattc gctccgtgtt caaactagcc tgcgtgatca
     5881 tccgcataac gtcgtggtga acggcgggcg tgcaccagtt gccgctatct cattatgctg
     5941 gcggcccggc cgttcgatct ggagttaggc actggttgcc tgatgcaccg agggcacctt
     6001 gcgatacatt gccaaaagat caagtggctt gggttccgga actcccagaa cagtgaggat
     6061 gcagtgcgct tgaccgcgat gatgggtctg gtgattgaac caatgtgcca acagatcccg
     6121 tagaatgaat agttgtggca ccccctgggt ggtccagtag ctgaaatctc tgccaatctc
     6181 ttcctcggaa agaccttcca cgtactgaac gatccgagca tcttcggccc tgcgcgcggc
     6241 cttaagcgac ggtaggtcgt cgaacatgat tgcgttgagc ttagagggat gctcgccctc
     6301 acctgtgaat ctacgcatcc agatccgatc ggttacaaga agatgattta gtgtaccgtg
     6361 caggctgcga aagaacgcgc ccacgtcctt tcggtaagac tcgtcggata acgcccaagc
     6421 ctctgcataa aggcgagcat tcgcccaggc gttgtactgg gccatcatag cgaagtgcgg
     6481 cttcatcggt gttgacctcc ctttcgagac gattcatcga tcgtcattag cctaaccgcc
     6541 gcggttcagc cgcgtgaggt acgagcgtcg gctggaatcg ttggttatgc agcatgcggc
     6601 ctcccctcat tctgaaggcg ctgaatactg cgcgaatacc tcggctccgg tcaggttctc
     6661 cgtttgattt gcaaacgaat agaacgacgg cttttcatcg ataaaaatct gttgatcaaa
     6721 aactcattgt tccccgtcat cgagtagacc aacaggaacc gcgtagtgcc cttctttctt
     6781 tagacggtag aaaagatggc ctccacactt actacagaag ccacgctcgg cccagtcgga
     6841 agaactgaag acagatatac tgtcactgcg ttcaaaatga acgtcgctac cgcactcaat
     6901 cacgagcaag ggcccacctg tccatttcct acacatactg caatggcagg cgcccacact
     6961 cttgctggtc gtctttattg agatacggac tgcgccgcaa aggcaactcc cactacgttc
     7021 ggtttcgcct gccatatcgt cctcctaaat tgagtgacga gttgcggcta agccgtggcg
     7081 aagaaagcga gtcttggagg cgagcatttt cgacatcagt cttaagccgc gggttagctt
     7141 cattgccgca cgttctttgg aatcgttcca ttcttccatt catcacactg atttattgca
     7201 gacggaagaa aaagcccggt tgtggttgac tgattaacca gacaaaaacc cagaaggaac
     7261 aacggatctc tgaccgtctt cgcaactacc gaatctgtac tctcaatgct ggatagatag
     7321 cccagaacac gaataatatg tccgggcttt atctgtcccg cctctggtct caccaaggag
     7381 cccacccaga tagatgtatc attatcattc ggctttagga gggataaccg aatcagcggt
     7441 ttgccttgat tcccttcttt gatttcttgg atcagaccag caagaccatc aactttccgt
     7501 cccatttccg gagttcaaat acgcatcacg attatcgttg ccggttactg gaacgggaaa
     7561 cggatcgtca gcgccgtaaa ggttcgctgc cgatgtcaca ccatcaaagt gatgatcaat
     7621 agtgcgcgtg agatacattt gaagctaacg caaggctgag caggcacccc gcgagttttg
     7681 cgagcgggtt cgaagatctg cctcaagcgt atgttcggca tgtcagtcac cggacgggcg
     7741 ctcctcgcgg gtgacctgga acgaccacgg ataagaaaaa tgaatctctg agaccgccgt
     7801 ttcttctgaa tcgtaaacac ttgcgccaac atcgtcagaa atccagcacc cttcgaactc
     7861 atcgctgcta aaatgctcgc tgcatcagct aaatagaccg tcgcctcgcg caatcaagta
     7921 cacccgctgc tgaccgttgg gatgtgtcca gcgcgaaacc gcaacgtttc cgtgaataaa
     7981 taaaggccac gtgtcaaagt tcttgccaga catagcttcc ttcgccaaca tcacttctat
     8041 gcctaacgcc gcggtgagcg gcgcgcgaag cgcgtccgtt cgactgcgaa gttatgcggg
     8101 ttcatgtgat ggcacagggt gcagccgatt ggattcctcg aagagcgcca gagccttctg
     8161 gagatcgaaa ggctgctcga tgagccgttc gggatcccat tcgctgcgtg ggagggcaaa
     8221 gaaatcgcca ccgtagacat gcaccgcccc cgtcaagcga ctaaccggat tcagaacaga
     8281 gtggatgacg tcatgaccga gggggacggc atcacgctca ctcagggctt tcgcgccagc
     8341 ggcttcgatt cggccatcgc cgcgttccgg catgcgtcgc cagaagatat tgtcctcacg
     8401 accggtgtaa ataccgatga cggcccacat cttatgattg tgcggcaact gactcatccg
     8461 cggtccccag acgacattca ggatagtcag ttcggctgtt cggaagagcg ggtgcaagcc
     8521 ggcccggcgc ggctcaccaa gagcccgtaa gatctcgccg ggctcggcga ccgcgcgagc
     8581 gacggcctca aggacgccgg ccgatccggc tgcagcgctc gcgtgctggc actcggcaat
     8641 aaacctatcg cggtcaaaca tgacggctca cctccccatc ggttcgacga tggatgttgc
     8701 tgaaccccgg cctttccgtc taacgcttga gttcagcggc gcgctttagc gcgtccgctg
     8761 aaacgaatgg ttatgtgtct gcgactgtta gtacgttctc gatccgtcga tccggtatga
     8821 gccacagcag cgcaacgaat acatacaggc ccaaggagat ccattgccac cagaaggcca
     8881 aaggaatggc agcgacgtac agcaacggcg acaacttgcc tttccaatcg cctccgatag
     8941 cctttttcag aattgagtcg ggaccctgcg aggcaatgat cagttgttgc aatatccagt
     9001 atgcgaccgc agccattaga agcacaacac catatagcgc tgatggtgca gccgcgaagt
     9061 ggttctcacc catccagccc gtcgtgaaag gtataaggga cagccagaac aagagatgca
     9121 gattcgccca tagcatcgct cccgtgactt tttgagtcgc atgaagcatg tggtggtggt
     9181 tattccaata gataccaagg tagacgaagc tcagaacgta actcaagaat accgggatca
     9241 atggcaccag agtctcgatg ccctcgccgt ggggcacctt catctccaac accatgatgg
     9301 tgatgatgat ggcgatcacg ccatcactga acgcttcaaa ccgattcttg cccattcaga
     9361 atgtgctcgc gcttaaagga acttccactt tacaaaaatc gccaccgcgg atggcaacaa
     9421 gaggatcaac aggaaaattg gtagctcttc tcgaaacgaa taccccgctt gcgacacacc
     9481 aatccacatg ttcgccgctg ccgcgataaa ccagatgacg agaaacgcga cggtcgctgt
     9541 gatcatagac gaagtccccg aactggtaag aagctttgca actccaagac acgctgccca
     9601 cagcacaaag cccacggcaa tgatgattgc ggtgcgcatt gctacctccc gtcgtttgct
     9661 cctgatcgcg agatggttac agacacataa caccaaggct gagcggccga gaccagcgag
     9721 cgcagcgagc gggtccgaag atccgtttca agccaattgt taggcgtcaa tcgtcccact
     9781 gcccgtctac tctagtcctc ggcctttagc gctctcgttc aaaacagaga gtacctacga
     9841 tttttttgcc aggaaggacg gcgctggcaa tttcgggggt gcgaatatcg agc
//
LOCUS       DASUTG010000852         5895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_725066, whole genome shotgun sequence.
ACCESSION   DASUTG010000852 DASUTG010000000
VERSION     DASUTG010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725066"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VF089_19860"
     CDS             <1..780
                     /locus_tag="VF089_19860"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176271.1"
                     /translation="GMSALASGETQFHVGSATGSSIGAMAGGVDLAFIAGLINKLDGT
                     FVVAPKIRAPEDLKGKRIGVQSIGGGVWMFTMLAFEKWGLNPERDNIQFRVLGDQSVI
                     AQAITQGIVDGAYLGYTFGAQMERQGYRVLADLAKIGVPYQGLGVMAKRSLIDRTPEV
                     AEKALRAMVESISFISNPANKALVLKSLSKELRLSKAEDAQAGYEVMRTLYDRRIYPN
                     AEGLRNVIRLLANSSEQMKKIKVESIVDDRIVRKLEKEGLF"
     gene            970..2031
                     /locus_tag="VF089_19865"
     CDS             970..2031
                     /locus_tag="VF089_19865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176272.1"
                     /translation="MAGNGDNAGFEVIISSDSHVMEPSEILVERVPKIFKDQAPRFPQ
                     LKLGESFQTHPGGSDPTHRITEMETDGVSAEILYPTYLLPHFAMDDAKLQAACFRAYN
                     DWLIDYCSTSPKRLLGIAAISVYDIDEAVKELERCAKAGVKGSIIWQAPHPDLPFHSE
                     HYNKFWEASQALDLPVHLHILTGHGYHKESVFGNKRTGVEAYRGSVNLKLKEIIDAFF
                     ELIFYGVLERYPKLKFVSVENEIGWMPFMLQQWDYYYRRFRGVNPLPITKDPSEFFKT
                     QIFGTFFRDTAAGHSFEWWGQDNCMWSNDYPHGNSTWPESRKYIDRDLGHLPPDIRKK
                     LVYSNVKNLYDIEIAHPLQ"
     gene            2311..4059
                     /locus_tag="VF089_19870"
     CDS             2311..4059
                     /locus_tag="VF089_19870"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6176273.1"
                     /translation="MPARYGSDLVVDLLKVLGIEYVALNPGSSFRGIHDSLVNYESGR
                     TPNPEMILCCHEEIAVAVAHGYAKATGKIMPAIVHNVVGLQHASMAIYNAWCDHTPVM
                     VMGGTGPMNSSKRRPWIDWIHTAQVQGNLVRDFVKWDDQPVGVEAFPSSIMRGYRIAM
                     SDPGGPVYLCFDVTDQEAAIDKEIPLPDPKRFRPAAPLQADYDAIKEAAEVLATAQNP
                     VILADYVGRNNEAVMSLVALADLLSIPVIDFGARLNFPNTHSLNLTGRNRELIADADV
                     IMGLDMTDLYGALVRSDPNTHANVPATKPGCKVINVTLKDIVVRSWTSDFQELAPVDL
                     PIVANTRVFLPALTEEIRKQGKFSKSVVDDRRKAMAGQHEEVHARWQADLKKRWDEKP
                     IAPPRLAYEIWQALKNEDWLLVAGAFRGWPSRLWTWDKPGLFLGGYGGGGLGYGPAAS
                     VGASLPYRGTGKICVNLQRDGEMLYTPSALWTAANTGVPLLTVMTNNRVYYNDVEHQE
                     KVAIHRGRPVENKLIGMEMAKPPVDFANLARTFSVHGEGPITEAEQIRPAVERAIKVI
                     KEEKRPALVDVYMQMV"
     gene            4230..5234
                     /locus_tag="VF089_19875"
     CDS             4230..5234
                     /locus_tag="VF089_19875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176274.1"
                     /translation="MIWNKFIVAALFAMICLAFAQPVLSQTKPLKKIRVGVPSVGMGN
                     IITFVIKEGKLFEKYGLDAEVITVMGSGIGSKALISGNLDVIPIATPTVIAANLAGAD
                     MAILAHTMPAVVHALMVRPDIKRPEDLKGKKIGVSSLGSLTDFLVRSIAKKKGLNPDR
                     DITLITTGGSDTERVMALKAGAVDGTAVSHPGYGLARKMGFSMLWDSAKELNYPWMEI
                     TTRRAAIKNDRELVTNYMKAHLEGIALFKTNRDFGIKVIKKYLKTPDDDLVSESYDIF
                     SKMFLPTPYPNHPGMKISYEYVAMTRKDIWNHKPEEFTDSSFVGELDKSGFIKRLYEK
                     "
     gene            5618..>5895
                     /locus_tag="VF089_19880"
     CDS             5618..>5895
                     /locus_tag="VF089_19880"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6176275.1"
                     /translation="MNDDVFTGQWSQVRGSLKSWWGRLTDDDLDRIGGQKDRLVGLVQ
                     EKYGRTREEAEREVEQRLREYGDGGGIGAKLKTGASEFGSKLSNKAREA"
BASE COUNT         1428 a         1534 c         1656 g         1277 t
ORIGIN      
        1 ggcatgtcgg ctttggcttc gggcgaaaca caattccacg tgggttccgc caccggctct
       61 tccatcggcg ccatggccgg cggtgtcgac ttggccttca ttgccggtct catcaacaaa
      121 ctcgatggca cgttcgttgt cgcgccgaag attcgcgcgc cggaggatct caagggcaaa
      181 cgaatcggcg tgcagagcat cggtggcggt gtctggatgt ttacgatgct ggcattcgag
      241 aaatggggcc tcaatcccga gcgggataac atccagtttc gagtgcttgg agatcagtcg
      301 gtgattgccc aggcgataac ccagggaatc gtcgacggcg cttatctggg ctacacgttt
      361 ggcgcgcaga tggaacgcca gggctaccgg gtgttggccg atctggccaa aatcggcgtg
      421 ccctatcagg gtcttggcgt catggccaag cgcagtctca tcgatcgcac gccggaagtt
      481 gccgagaaag ccctgcgagc tatggtggag tccataagct tcattagcaa tcctgccaac
      541 aaagccttag tactaaaaag cttgagcaaa gaactgcgcc tgtcgaaagc cgaggatgcc
      601 caggccggct acgaggtgat gcgcacgctt tatgatcggc gcatctatcc caatgccgag
      661 ggcttgcgta atgtaatccg cctgctcgct aacagcagcg agcaaatgaa aaaaataaaa
      721 gtcgaaagta tcgtcgatga tcggatcgtc cgcaaactgg agaaggaggg cttgttctaa
      781 tcgggcatcg tgctctgaac gtgttcgtga tcgtgacggt gaatgccaaa aattcagaac
      841 acgaacacgt gacacgaaca cggctttgaa tacccgcaat cttgacggtt caataggtgc
      901 ggtgatacag cttcagatag agcgataaat actgaatacc taatcagtga cacccaagga
      961 gggacgttta tggctggcaa tggtgataat gcgggcttcg aagtaatcat ctcttccgac
     1021 tctcatgtga tggagccgtc tgagatcctg gttgagcggg tgccgaagat tttcaaagat
     1081 caggcgccgc gtttccccca gttgaaactc ggtgagagtt ttcaaactca tcccggcggt
     1141 tcggatccga cgcaccgaat cactgagatg gaaaccgacg gcgtcagcgc cgaaatcttg
     1201 tatccaacgt atcttttacc gcactttgcg atggacgatg ccaaactaca ggctgcctgt
     1261 ttccgagcct ataacgattg gctgatcgat tattgcagca catcgcccaa gcgtttgctc
     1321 ggcatcgcgg ccatttcggt ttacgacatc gacgaagcgg tgaaagagct tgaacgctgc
     1381 gccaaagctg gtgtgaaggg ctcgatcatc tggcaagcgc cgcatccgga cttgccgttt
     1441 cattcggagc attacaataa attctgggaa gcgtctcagg cgctggattt gccggttcac
     1501 ttgcacattc ttaccgggca cggctatcac aaggagtcgg tgttcggcaa caaaagaacc
     1561 ggagtcgagg cctaccgcgg cagcgtcaat ttaaaactaa aagagattat cgatgccttc
     1621 tttgaattga tcttctacgg cgtcctcgag cgctatccca agctcaagtt cgtcagcgta
     1681 gaaaacgaga tcggctggat gccgttcatg ctgcagcagt gggattatta ctaccgaagg
     1741 ttccgcggcg tgaatcctct gccgatcacc aaagatccca gcgaattctt caagacccag
     1801 atcttcggaa ccttcttccg cgacaccgcc gcgggtcata gttttgaatg gtggggccag
     1861 gataactgca tgtggtccaa cgattatccg cacggtaact caacctggcc ggagtcgcgc
     1921 aagtatatcg accgcgatct cggtcacttg ccgccggata tccgtaaaaa gctggtctat
     1981 tcgaacgtca aaaatcttta tgacatcgag atagcccatc cgctgcagta ggagcgcgat
     2041 ttgctgatac caacgatcgg cgcgctagtg gacggagagg tctgccggcg cgcttttctt
     2101 tggcggtgat agcacaagtg cgcacgaaca aaaatcgaag cacaaccgaa attcgaaacg
     2161 atccacatat taaagaatcc gcaattttca agacaagcat cgttcgatgg ggccatttct
     2221 gaatttttcc tgattgaaga tttgccttat ccgaatccca ttttgatatt aggatttcga
     2281 atagttttca ctcgaaatta ggaggttttt atgccggcac ggtatggttc cgatctcgtc
     2341 gtcgatctgc tcaaagttct cggcatcgaa tacgtcgcgc tcaatcctgg ctcgagcttt
     2401 cgcggcatcc atgattctct agtcaactac gaaagcggcc gcacgccgaa tccggaaatg
     2461 atcctctgct gccacgaaga gatcgctgtg gcggtggcgc acggctatgc caaggcgacg
     2521 ggaaagatca tgccggcgat cgtgcacaac gtcgtcggcc tgcagcacgc ttcgatggca
     2581 atctataatg cctggtgcga ccacaccccc gtgatggtga tgggcggcac cgggccgatg
     2641 aactcgagca agcgacggcc ttggatcgac tggattcaca ccgcgcaagt gcaaggcaat
     2701 ctggtgcgcg acttcgtcaa gtgggacgac caacctgtcg gagtcgaagc tttcccctcg
     2761 tcgattatgc gcggctatcg cattgccatg agcgatcccg gtggtccggt ctacctctgc
     2821 ttcgatgtga ccgatcaaga ggcggcaatc gacaaggaga tcccgctacc tgatccgaaa
     2881 cgatttcgtc cggcagcacc cctgcaagcg gattacgacg cgatcaaaga agcggcggaa
     2941 gtattggcca cggcgcaaaa cccggtgatc ctagccgatt acgtcgggcg caataacgaa
     3001 gcggtgatga gcctggtggc cctcgccgac ctgctgtcga ttccggtaat tgatttcggc
     3061 gcgcgcttaa actttcccaa tactcattct ttgaatctca ccgggagaaa tcgtgaactg
     3121 atcgctgacg ccgatgtgat catgggcctg gacatgaccg atctctatgg cgcgctggta
     3181 cgttcggatc cgaacacgca cgctaacgtt ccggcgacca aaccgggctg caaagtaatc
     3241 aacgtcacgc tcaaagatat cgtagttcgc agctggacca gcgacttcca ggaattggcg
     3301 ccggtggatc tgccgatcgt cgctaacacc agagtctttc tccccgcgct caccgaagag
     3361 atccgcaagc aggggaagtt ttccaaaagt gtcgtcgatg accggcgcaa ggcgatggcg
     3421 ggccagcatg aagaagtgca cgcgcgctgg caggccgact tgaagaagcg ttgggacgag
     3481 aaaccgattg cgccgccgcg cttggcctat gaaatttggc aagcgctcaa gaacgaggac
     3541 tggctgctgg ttgccggcgc tttccgcggc tggccgagcc gtctctggac ctgggataag
     3601 ccggggctat ttctaggggg ctatggcggc ggcggtttgg gctatgggcc cgcggcgtct
     3661 gtcggcgcat ccctgccgta ccgcggcaca ggcaagatct gcgtcaacct gcaacgcgac
     3721 ggcgagatgc tgtacacgcc gagcgccctt tggaccgccg ccaacaccgg cgttccgctg
     3781 ctaacggtga tgaccaataa ccgcgtttac tacaacgacg tggagcatca agaaaaagtt
     3841 gcgattcacc gcggccggcc agtcgagaac aaactcatcg gcatggagat ggccaagccg
     3901 ccggtggatt tcgccaacct ggcccgtacc ttcagtgttc atggcgaggg accaatcacg
     3961 gaagcggagc agattcgccc ggccgtggaa cgggcgatca aggtcatcaa ggaggagaag
     4021 cggccagcct tggtcgatgt ttatatgcag atggtataac tgggaatcgt tccaatcgtt
     4081 caagtcgttc aaattgttcc agacgtaaga acaaacacgc gcaacacaca aacttctgtg
     4141 aacaccggtt cggacccgtc cactgacgtg gagaaagagc aaaggcgata cgaaatagcg
     4201 ctagccgggc acctttagga ggatgtttca tgatttggaa taagttcata gtcgctgctt
     4261 tgtttgcgat gatctgtttg gctttcgccc aacctgttct ttcgcagact aaaccgctca
     4321 aaaagattcg cgtcggtgtg ccgtccgtcg gcatgggaaa catcatcacc ttcgtcatca
     4381 aagaaggtaa gctgtttgaa aagtacgggc tcgacgccga ggtcatcacg gtcatgggct
     4441 ctggcatcgg ctcgaaagct ttgatcagcg gtaacttgga cgttattccg attgccacgc
     4501 cgactgtgat cgcggccaat ctcgcgggag cagacatggc gattttggcg cacaccatgc
     4561 ccgctgtagt tcacgcgctg atggtacggc cggatattaa aagaccggaa gatttgaaag
     4621 gcaaaaagat cggcgtctct agcttgggat cgttgacgga ttttttggtc cgttcgatcg
     4681 caaaaaagaa ggggctcaat cccgatcgcg acatcactct tatcaccacc ggtggcagcg
     4741 atacggaacg ggtcatggcg ctcaaagccg gcgcggtcga cggcaccgcg gtgtcccatc
     4801 ccggctacgg cttagctcgg aagatggggt tcagcatgct gtgggattcc gccaaggagc
     4861 tcaactaccc atggatggaa atcaccacga ggcgggcggc gattaaaaat gatcgggaac
     4921 tggtcaccaa ttacatgaag gcgcacctag aaggcatcgc gttgttcaaa accaaccgcg
     4981 atttcggcat caaagtcatt aaaaagtacc tgaaaacgcc tgatgatgat ttggtgagtg
     5041 aatcttacga catcttttca aaaatgtttc ttcccacgcc gtatcccaat catcctggga
     5101 tgaagatcag ctacgagtat gtcgccatga ctcgcaagga catctggaac cacaaacccg
     5161 aggagttcac cgactcgagc tttgtcggag agttagataa gagcgggttt atcaagaggc
     5221 tctacgaaaa gtaacttgct ccgccacctc cggctgggaa agatcaaaat tttggcaacc
     5281 ggtaaacgag gcggcgtgga tttcgctagt acccggcggg gtgtcttgag acggcgccgc
     5341 gaatcaatag tcaaaaacag ctagcgttcc acagataacg cgagccataa acctaactgg
     5401 tcggtcatct ttgtagctat tgaaaacagc ggaatggacc gagatgaccc agcgactcgc
     5461 tcctgacaca gctgcgagct ttgtgtctta aatttcggac aattgtgtga aaactgtcaa
     5521 aatagtcagg ggttttatct cacttcggtg gcatatcctt tgccgttatt gttccctaac
     5581 gcggcttata tgacaaattc gaatggaggt attttccatg aatgatgatg tattcacagg
     5641 ccaatggagt caggtgcgcg gatcactgaa atcttggtgg ggtagattaa ccgatgatga
     5701 tctcgataga atcggcggtc aaaaagaccg gttggtgggc ttagttcaag agaaatacgg
     5761 acgaacgcgc gaagaggcgg aacgtgaggt ggagcaacgt ttgagggaat acggcgacgg
     5821 cgggggcatc ggagccaagc taaaaaccgg cgccagcgaa ttcggatcga agttatccaa
     5881 caaagcgaga gaggc
//
LOCUS       DASUTG010000853         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_280850, whole genome shotgun sequence.
ACCESSION   DASUTG010000853 DASUTG010000000
VERSION     DASUTG010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280850"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..282)
                     /locus_tag="VF089_19885"
     CDS             complement(70..282)
                     /locus_tag="VF089_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176276.1"
                     /translation="MTIADLIAKLSRYPASARVTLLDPGRRWLLPIEITALSADGMSR
                     EVDFIAITADSASDEIEGLAAAKRKA"
     gene            complement(636..1589)
                     /locus_tag="VF089_19890"
     CDS             complement(636..1589)
                     /locus_tag="VF089_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003591380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6176277.1"
                     /translation="MPESLPRTKEERRLKRAAHREQQRQTNIGRAREMHLARLDREKN
                     TDPLPMIAGDSKTRQLLIGCSGWFYWHWRGAFYPADLPTNRWFPHYAGRFKTVELNAP
                     FYSWPTVATVESWIRQAGRRQFVYTVKVCELITHVKRFAGTKTLVRDFGLIADLLGPR
                     MGCFLFQLPPSFRYTPARLKRILNQLDHRRRNVVEFRHKSWWNEAVYKAFRETGTIMC
                     SCSGPRLPDELVKTAEDIYIRFHGLKNWYRHDYSTAELAVWAKRIEESGAKTVWAYFN
                     NDRDGYAIKNARALMRLLEPGTQRAKARKSPALGSGPAADS"
     gene            complement(1582..2112)
                     /locus_tag="VF089_19895"
     CDS             complement(1582..2112)
                     /locus_tag="VF089_19895"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX6176278.1"
                     /translation="MSNSILNADKKLKAHAQEALAALKAANVTIVTAESCTAGTVAAL
                     LAQAEGSSDILHGGFITYTKEHKTKGLGIPWRLLKEQGAVNGDVVKQLAAGALERSPA
                     SMAIAISGVLGPEPDEDGNPVGLVYFCLIRKDKEPTVVKEEFGNQPHEELLEKALHRA
                     FALIEASVSANARKHA"
     gene            complement(2199..2491)
                     /locus_tag="VF089_19900"
                     /pseudo
     CDS             complement(2199..2491)
                     /locus_tag="VF089_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
BASE COUNT          484 a          717 c          720 g          570 t
ORIGIN      
        1 gcgtttttca tgatgcactc cgcgtttcaa atggttttgc caaaggtaaa aaattaggcg
       61 ccgagctctt caagcttttc tcttcgcggc cgctaagcct tcaatttcgt cgctcgcgct
      121 gtccgctgtg attgcaatga aatcgacttc acgactcatt ccgtccgccg atagtgccgt
      181 tatctcgatc ggtaaaagcc atcgcctgcc gggatcaagc aacgtcactc gggcactcgc
      241 cgggtatcga gacaactttg cgatcagatc ggcgatagtc atagcgcctc caatttcggg
      301 tcggtccatg ccagtaatca acttgcttga gaagagcaga ctgatctcga ctcatcgcag
      361 ccaatcttgg cctcgatcgc acccgcctca atcgcaccgt ccttgaccca aagagctgcg
      421 cccgtcgtat acgcggacaa caaaagcggc ggcgaagcta gtcacagtca gggggccaat
      481 gtgaggctcc gccgccttgc gaccgaccgc gaggggaagg agttcggtcg ccattgccga
      541 ggttacaact acactccgta cgatttcgga caaaagcaca gacatacccg ccaacagttc
      601 tcaggatgtt ttgttcaagc cggcagatcc atttatcacg aatctgctgc cggacctgaa
      661 cctagagccg gcgatttcct tgctttcgcc ctctgggttc ctggctctag cagccgcatt
      721 agtgcgcgag cattcttgat ggcgtatccg tcgcggtcat tgttgaaata agcccaaacg
      781 gttttcgcgc cgctctcttc gatgcgtttc gcccagacgg ccaactccgc agtggaatag
      841 tcatgccgat accagttctt gaggccgtga aagcggatgt aaatgtcctc cgccgtcttg
      901 accagttcat ccggcagccg gggaccgcta cacgagcaca tgattgttcc tgtctctcgg
      961 aaggctttgt aaactgcctc gttccaccag ctcttgtgcc ggaattccac gacgttgcgt
     1021 cgtctgtgat caagctggtt cagaattcgc tttaggcgcg ctggtgtgta gcgaaaactg
     1081 ggcggtagct ggaacagaaa acaccccatg cgcggtccaa gcagatcggc tatcaggcca
     1141 aagtcacgca ccaaagtctt ggtcccggca aagcgtttaa cgtgagtaat aagctcgcag
     1201 accttcacgg tgtagacgaa ttggcgtctt ccggcctgcc gtatccaaga ttcgacagtg
     1261 gcaacggtcg gccatgaata gaaaggcgca ttcagttcca cggtcttgaa gcgtccagca
     1321 tagtgcggga accaacggtt tgtcggcagg tccgcaggat agaaagcgcc gcgccaatgc
     1381 cagtagaacc agcctgagca accgatgaga agctggcggg tcttgctatc gccagcgatc
     1441 atcgggagag gatctgtgtt cttctcccga tcgaggcgcg caagatgcat ttctcgggcg
     1501 cggccaatat tcgtctgacg ttgctgttcc cggtgtgcgg cccgcttgag tctgcgctct
     1561 tctttggtgc gggggaggct ttcaggcatg cttccgcgcg ttggccgaaa cgctggcttc
     1621 gatcagggcg aacgcgcggt gaagggcttt ctcaaggagc tcttcgtgcg gttggtttcc
     1681 aaattcctct ttcacaaccg tcggctcttt gtctttccgg ataagacaga agtacacgag
     1741 gcctacagga ttgccgtcct catctggttc ggggccgagc acgccgctta tggcgatggc
     1801 catagaggcg ggtgagcgtt cgagcgcacc ggctgcgagc tgtttgacta catctccgtt
     1861 cactgcgccc tgttccttga gcaggcgcca cgggattccg agccctttgg tcttgtgttc
     1921 cttcgtatag gtgatgaacc cgccatgcag gatgtcgctg ctgccttcgg cctgcgcgag
     1981 cagggccgcg acggttccgg cggtgcaaga ctcggcggtt acgatggtga cgttggcggc
     2041 tttgagcgcc gcgagagcct cctgcgcgtg ggcctttagc ttcttgtctg cgttgagaat
     2101 tgagttcgac acgtcgactc tggctccgtc aacaaactca gtgctggatt aggcaccgtc
     2161 gttgatttag acgactattg acaagcgttt gcggtcaaca ccgggatgtt gctgccgacg
     2221 atgtcccggt tgccttcctt ggtgtagagc ttgaccgcga agccgcgcac gtcgcgcgcc
     2281 aggtccatcg agcccttgct gccggcgacg gtggagaagc gcacaaatgc cggactcttc
     2341 tcgccggcgc gctggaagat gtcggcgctg gtgtagcgcg acagcgactt gtaggtctca
     2401 aggtacccat gcgcgccgta gccgcgggcg tgcaccacgc gctccgggat gcgctcgtgg
     2461 tcaaagtgga aatcctttcc cggaaatgga a
//
LOCUS       DASUTG010000854         9415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1762746, whole genome shotgun sequence.
ACCESSION   DASUTG010000854 DASUTG010000000
VERSION     DASUTG010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762746"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VF089_19905"
     CDS             complement(<1..872)
                     /locus_tag="VF089_19905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176279.1"
                     /translation="MAKLPLEGVRVIELTTGAAGPTVARVLCEFGAEVIRCETRLRGD
                     GHRGEDPKLWNKKPDFMKLQRGKKSFTVNMQTPKGRDLVKQLLKESDVLVENFGLGIL
                     EKWGLDWPQLKDINPRCILIRVKGMGCTGPHAADLTYGPNVGNTMGTTYLWNYPGATT
                     ATAEPRTQHPDFMGGVTGAFAVVLALIHRKKTGRGQWIDSAQVEIGASLLGPKYLEYS
                     VNKREPQPQGNRSLLAAPYGPYQCSGNDRWCVISVYSDEEWERLAGLLEKSGLRREPK
                     FATHLQRVRHKAEL"
     gene            complement(1031..2242)
                     /locus_tag="VF089_19910"
     CDS             complement(1031..2242)
                     /locus_tag="VF089_19910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176280.1"
                     /translation="MSEYNHEPPLAGCRVLDIGGPLALHCTKLLADMGAEVIKIEPPS
                     GDQSRRVPPFKDDSPHPEKSLYFLHFNTNKRGITLDIEKADGRAILLELVNKADVVIE
                     TFRPSRARELRLSYQDLSASNPGLIVASITPFGQSGPWRDYKADDIAGLALGNLLYLA
                     GEPGEPPLQPPGEIAYGMASTYGAFGIATALYHRLESGRGQHIDVSMHECSGHIAGYF
                     IPNYGYTGAKPARASRKGEETDLYDPYATKNGYARIFIIPVEQWRRLVDWMGRPPAIS
                     GPEFEKMAYRRQHPDVVVGAIAEFCARHTKEELYEEGQKRRIAVTPINTAGEFINSEQ
                     VKERKIFVEMEHPIIGKYAHFGPVPRLSESAGSISRPAPLLGQHNREIYCGELGMSND
                     DLVALRASEVI"
     gene            2592..3596
                     /locus_tag="VF089_19915"
     CDS             2592..3596
                     /locus_tag="VF089_19915"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176281.1"
                     /translation="MLRAINFVSSCLIVAALLSAGQAFSATRLEPLTVGYSNITATYA
                     PLWIAVEERVGIKYELDLKAIYAGRVRPQQLLATGDVPVVIASGTGTMTSHIVGVKDQ
                     VLVATITSKIGTSLFAKAEIRTVEGLKGKTVATGRPAAFLDATVRYVLRSKFGLIPDR
                     DVKLLPTGEPYLGLQALERGIVDAAAMSIPHLFIARKAGFKELVSFDKLGIEYPYTSV
                     VVLRQTAAKSPDLVERVIKCMVEGIHIFKTDKAKSLAAMRLYMKGADEGILEESYQHT
                     RSTIDEAPHPSLQVVNGGLEMLSLQYPQAKQTDASMIIEPTFMKRIDESGFVRALYKR
                     "
     gene            3702..4742
                     /locus_tag="VF089_19920"
     CDS             3702..4742
                     /locus_tag="VF089_19920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176282.1"
                     /translation="MLDGKLRIAPTCYHMLHQVPVMTAHEMNFLYDEGLVTPHGTLGY
                     EILTESMVPFGLEKLGISQAMKEKSIDIALDVQSRTVFYQRARGADLYILCGWRNQHT
                     NVWMAPPHINSLQELKGKRVGISDFNSIRHWAIQIQLKKAGLDLERDIEWVRLGVNSR
                     FHVDAIRNGKVECAPVPPWSAEDLKKEGCNALVSPADQYPNGRPERIIAVTGRILEAR
                     PDLVKSFLRGIIRSYWFIRDMPKNYEYVNNLEKRLRLKSADPEERIVTHLRTPRDLEA
                     MPFPLNGRATGFEDMLKEEERLGELNYQVPPIQEVAAQDLVDEAYKELRGRKELAEEY
                     ERVSKVEQRWGY"
     gene            4871..6313
                     /gene="glmL"
                     /locus_tag="VF089_19925"
     CDS             4871..6313
                     /gene="glmL"
                     /locus_tag="VF089_19925"
                     /inference="COORDINATES: protein motif:HMM:NF040745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HEX6176283.1"
                     /translation="MALLALLIDFGSTFTKVVAVDLNAVQVVGRSQAPSTVLTDVREG
                     LLQALSALHERHSLFDQRPSSLDILSGHLALASSSAAGGLRMVVIGLVPGLTLLAARQ
                     AALGAGAKVVGSFAFKLASNSIDEIARLRADMVLLTGGTDGGDSATILHNAHLLSDSL
                     ISVPVIVAGNQAVAPEICRILQAGGKEVRLTDNVMPRSGVVAVDPARDEIRKLFVERI
                     THARGMSAITELVSVVLPTPMAVLRGVTLGAVGSDQEEGWGEMVVVDVGGATTDVHSI
                     GYGYPAGENVITQGIPEPYAKRTVEGDLGIRFNAATLLGQVGPDRFEVEWRSMFPHLQ
                     VSRDEIGRYINHISENTARVPEEKWHRAADAVLARVAADIALERHVGKRERVVTSAGE
                     AWVHYGKDLRNTPRLLATGGVFVHNPYASMILSGSAQDQTDVTVLRPKQPQLYLDSSY
                     LLYAVGLLGGDYPDVAVRLFKTYMTPVAQS"
     gene            complement(6445..7965)
                     /locus_tag="VF089_19930"
     CDS             complement(6445..7965)
                     /locus_tag="VF089_19930"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6176284.1"
                     /translation="MYNEFFGFLAPPFSATPDSRIFYDNELYREAFANLRYGIEWRKG
                     LIVLTGDVGTGKTTLLDKIIRDLGSTIQVIFISYDQLNSSELIQLVSRELGLVSDRQD
                     RVSSLEQLRHYLIAQFRRRRIVALLIDEAQNLTEETFETIRFFSNFQYGGEKLLQIVL
                     TGQPELQYRLNQPKLHHLKQRIVIHSRLTPLTNDEVTRYILFRVRGAGFRGNDLFDPA
                     VLADISLFSGGIPRLINIICDNALLLAYADGKKTVTSEIVQEVARDLGLANFSESNRR
                     LAYAAADIRDANLQANPATSSGPIAFPEQHGRSRSFAWVPTGMVLAVIGLGGAGGALY
                     SQQIKGYLGQEPSLGNRSLPPLPLYQNRSVTVQRREPELSLNLRQSVTPVQAKPEDLP
                     KNPVAPQTPAPNPEQPQPQESSAKKRDSRLGTFEVVGQPSFVRGSARAEAKIIAMLEP
                     STRIKVISAKGDYLQVQAVFEGKTIRGYVHREDAFFERPQKGKRPPKGSSVVSGVN"
     gene            complement(8314..9408)
                     /locus_tag="VF089_19935"
     CDS             complement(8314..9408)
                     /locus_tag="VF089_19935"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6176285.1"
                     /translation="MAPAQSQAPRSPTPAPLNAKLYVETVAKALEHPWGLTFLPDGFM
                     LITERPGRMRKVDHAGILSKPPEGIPRVLARGQGGLLDVSVDPRFRDNRLVYVSYSEP
                     GENGAAGTSVARGRLGETGLKDVEVIYRQQPKVSGPNHFGSRLVFANDGSLFVTQGDR
                     FAYRDQAQDLSSGIGKIVRINPDGSVPKDNPLVDRKGAKPEIWSYGHRNIQSAAIDPQ
                     TGQLWTVEHGARGGDELNHPQAGKNYGWPIITYGVDYSGAKIGEGTAKPGMEQPVYYW
                     DPVIAPSGMIFYTGDIFPDWKGSMLVGSLNPGLLVRLVMKDGRIAREERYLSELNERI
                     RDVRQGPNGFVYLLTDSRDGRLLKVGPGRR"
BASE COUNT         2070 a         2505 c         2651 g         2189 t
ORIGIN      
        1 tccaactctg ctttatggcg cacccgctgc aggtgggtgg caaatttagg ctcgcgccgg
       61 aggccagatt tttccagcaa acccgccagg cgctcccact cctcatcgct ataaactgag
      121 atcacgcacc aacggtcatt gccgctacat tggtagggac catacggagc ggccaataag
      181 ctacggttgc cctgtggctg cggctcgcgc ttgttgaccg aatattccag atacttgggt
      241 ccgagcagcg acgcgccgat ctccacctga gcgctgtcga tccactggcc gcggccggtc
      301 ttcttgcggt gaatcagcgc caatactacg gcgaaggcgc cggtgactcc acccatgaag
      361 tcgggatgct gagtccgtgg ctcagcggtg gcggtagtcg cccctggata gttccacaga
      421 taggttgttc ccatggtgtt gcccacgttg ggcccatagg taagatcagc cgcgtgcggg
      481 ccggtgcagc ccatgccttt gacccgaatt agaatgcagc gcggattgat gtctttcagt
      541 tgcggccaat ccaagcccca tttttccagg atgcccaggc cgaagttttc caccagcaca
      601 tcggattcct tcaacagctg tttgactagg tcgcgaccct tgggcgtttg catgttgacg
      661 gtaaatgatt ttttgccgcg ctggagcttc atgaagtccg gcttcttgtt ccacagcttc
      721 gggtcctcac cgcggtggcc gtcaccgcgc aagcgcgttt cacatcgaat cacttccgcg
      781 ccgaactcgc ataacacccg cgccaccgtc ggcccggccg cgcctgtggt cagctcgatg
      841 actcgaactc cttcaagagg taacttggcc atagattcct tctctaaaga ggcttatgca
      901 ccgccgaggc gcggagtacg cggggtggat aattccttgt aaacgatgct tgaactctgc
      961 aactcgggtc tctgcggtga actacattgc cgcttaccgg ctaatggagt tgggatgaga
     1021 ttcttaccca ttaaattact tcgctcgccc ggagcgccac gagatcgtcg ttgctcatgc
     1081 ccagctcgcc gcagtagatt tctctattgt gctggccgag cagcggcgca ggccggctta
     1141 tgctgcccgc cgattcgctc agtctcggca ccggaccgaa gtgcgcgtac ttgccgatga
     1201 tgggatgctc catttcaacg aatattttcc gctctttgac ttgctcgcta ttgatgaatt
     1261 cacctgctgt attgattgga gtaaccgcga tccggcgctt ctggccttcc tcatagagtt
     1321 cctctttggt atgccgggcg caaaattccg cgatcgcgcc gaccaccacg tccgggtgtt
     1381 gcctgcgata agccatcttc tcgaactccg gtccggagat cgcaggtggc cgccccatcc
     1441 aatctaccag ccggcgccat tgttcgacgg gaatgatgaa gatgcgcgcg taaccattct
     1501 tggtagcata gggatcgtag aggtcggtct cttcgccttt gcgcgaagcg cgcgcgggct
     1561 tcgcgccggt gtaaccgtaa ttaggaatga aatagccggc gatgtgcccc gaacattcgt
     1621 gcatcgaaac atcgatgtgc tggccgcggc cgctctccaa ccgatggtac agtgccgtgg
     1681 cgatgccgaa tgcgccgtag gtggatgcca tgccgtaagc gatttctccg ggaggttgca
     1741 gcggcggctc ccctggttcg cctgccagat acaacaaatt gcctagagct aatccggcta
     1801 tgtcgtcggc cttgtagtcg cgccaggggc cagactggcc gaagggagtg atcgaggcga
     1861 cgatcaatcc ggggttgctt gcgctcagat cttgatacga aaggcgcagc tctcgggcac
     1921 gcgagggacg aaaggtttca atgacaacgt cggccttatt caccaattcc agcaggattg
     1981 cgcggccatc ggctttctcg atgtccagcg tgatgcctcg cttattggta ttgaagtgga
     2041 gaaaataaag actcttctcc ggatggggcg aatcatcttt gaatggtgga acacgccgtg
     2101 actgatcgcc ggaaggtggc tcaatcttga tcacttccgc gcccatgtca gccaaaagtt
     2161 tcgtgcagtg gagcgccaat ggcccgccga tgtcgaggac tcgacagccc gctagcggcg
     2221 gctcatgatt atattcactc atgaagtttt cccatttaaa aacgatgtgc gatagtcggt
     2281 acagttactg cggatcaggg aacgctgtca agcagcgcac atattttatt ctacttgact
     2341 ggcaatcgtt gtaagtcgta tcggcattta gcgaggtcgg ctgtattgct cacaaggaat
     2401 tgtagtggcg ccgaggcaaa ttgctggcga gctttgtaga gtaggggtgg atcccgcttt
     2461 cagctcacgg tcattccgat atacccgagt gcaagaatac gctggatgag gccgagcacc
     2521 tcgataaaat ccgttgacat aaaatcggct caatgataac cggcaaagtt aaccttcaat
     2581 ctggaatcac tatgttgcga gcaatcaact ttgtctcctc atgtctaatc gtcgccgcgt
     2641 tgctctcggc aggacaggcg ttcagcgcca cacgccttga accgctgacc gtcggctatt
     2701 ccaatattac cgcgacgtat gctccgctgt ggatagcggt cgaggagcgt gtcgggatca
     2761 agtatgaatt agacttgaag gcgatttacg ccggccgggt gcggccccag cagttgctcg
     2821 ctacaggcga tgtgccggtg gtcattgcct cggggaccgg caccatgacc tcgcacatcg
     2881 tgggcgtcaa ggatcaggtg ctggttgcaa ccattacaag taagatcggc acatcgctct
     2941 tcgccaaagc cgagatcaga actgtcgaag gtttgaaagg taagacggtc gccaccggcc
     3001 ggcctgcggc ttttttggat gcaacggtga gatacgtttt gcgcagcaag ttcgggctta
     3061 tccctgaccg tgatgtgaag ttgttgccca ctggcgaacc ttatctcggt ctgcaggcgc
     3121 tggaacgcgg tatcgttgat gccgctgcaa tgtcgatacc gcacttattc atcgcgcgaa
     3181 aagccggctt caaggagctg gtaagcttcg acaagctagg catcgaatat ccctatacca
     3241 gcgtggtggt gttgcggcaa acggcagcca agagcccgga cctagtagaa agagttatca
     3301 agtgcatggt cgagggaatt catatcttca agaccgacaa agcgaaatcg ttggccgcaa
     3361 tgaggctcta tatgaaaggt gccgacgaag gaatcctgga agagtcttat caacacactc
     3421 gcagcactat agacgaggcg cctcaccctt ccctgcaagt tgttaacggg ggattggaga
     3481 tgttatcgct gcagtatccg caggcaaaac aaaccgatgc ctcgatgatt atcgagccga
     3541 cgtttatgaa acggattgac gagagcggct ttgttcgggc gctatataaa agataaatca
     3601 tagccatgac atttcaattc ctcctatcat ctgcgctcgc cgttaagggc gaatagtaat
     3661 taaagctatc attcgcgtcc attcagaaag cgagaaccgc catgctcgat ggtaaattga
     3721 gaatcgcgcc tacctgttat catatgctgc atcaagtgcc ggtcatgacg gcgcatgaga
     3781 tgaatttctt atatgacgag gggctggtta cgccacacgg aacactcggg tacgagatct
     3841 taaccgagtc gatggtcccg ttcgggcttg aaaagctcgg catttcgcaa gcaatgaaag
     3901 aaaaatccat cgacatcgcg ctggacgttc agtcgcgcac ggttttctat cagcgtgccc
     3961 gcggcgccga cctgtacatc ctgtgcggct ggcgcaacca gcataccaac gtgtggatgg
     4021 cgccgccgca tatcaattcg ctgcaagagt tgaagggcaa gcgggtcggc atcagcgatt
     4081 tcaatagcat tcgccattgg gcaattcaga tccagttgaa gaaggctggc ttggatttag
     4141 agcgcgatat cgagtgggtg agactcggcg tcaattcgcg atttcacgtc gacgcgatcc
     4201 gtaatggcaa agtcgaatgc gccccggtgc cgccttggag cgccgaagat ctcaagaaag
     4261 agggctgcaa tgcgctggtt tcgccagcgg atcaatatcc caacgggcgt cccgaacgga
     4321 tcattgcggt taccgggcga atccttgagg cgcggcctga tttggtcaag agttttctca
     4381 ggggaattat ccgcagctac tggtttatcc gcgacatgcc gaagaattat gaatacgtca
     4441 acaatctgga aaagcggctc cggctcaaaa gtgcggatcc ggaagagcgg attgtaacgc
     4501 acctgcgaac cccgcgcgat ctggaagcca tgccatttcc gttaaacggc cgggccacag
     4561 gcttcgaaga tatgctcaaa gaagaagaac ggttgggcga gctaaattat caggttccgc
     4621 ccatccaaga ggtggcggca caggacttgg tggatgaagc ctataaagaa ttgcgcggac
     4681 gtaaggagct cgccgaagag tacgaacgtg tcagtaaagt cgagcagcgc tggggctact
     4741 aacacggatt cgaggtcgcg cggtgtccag ctccgaccgt taacaagttc gggcttttgg
     4801 gtcgcggagt tcatcctatg gcgcaattgc gaggatcggt gtccggctga ttatttattc
     4861 ttatcgttag atggcattgc tcgctcttct catcgacttc ggcagtacct tcaccaaagt
     4921 cgtagccgtt gaccttaatg cagttcaagt agtgggtagg agccaggctc cgagcacggt
     4981 gctcaccgac gtgcgtgaag gattgctcca ggcactgtcc gcactgcacg aacgccactc
     5041 actgtttgac cagcgaccgt cgagcctaga catcttgagc ggtcacctgg cacttgcttc
     5101 gagcagcgcc gcgggcggct tgcgtatggt tgtgatcggc cttgttcctg gcttgacact
     5161 ccttgcggca aggcaggcag ccttgggagc gggcgcgaaa gttgtcggct catttgcgtt
     5221 taagctggcc agcaactcca tagacgaaat tgcccgcctg cgcgcggata tggtgcttct
     5281 gaccggcggc accgacggtg gagatagtgc gacaattttg cacaatgcgc acctgctttc
     5341 ggattctttg atatcggttc cggttattgt cgccggaaac caggcagttg ctccggaaat
     5401 ctgccggatt ctacaagccg gcggcaaaga agttcgtctg accgacaatg tgatgccccg
     5461 ctccggagtc gttgcagtag atccggcgcg cgatgagata cgcaaactgt ttgtcgagcg
     5521 aatcacgcac gcacgaggaa tgtcagcgat caccgaatta gtttcggtcg tgctgccgac
     5581 ccctatggcg gtgctcagag gggtaacgct cggtgccgtt ggaagcgacc aagaagaggg
     5641 ttggggcgag atggtcgtgg tcgacgttgg tggcgcgacc acggacgtgc attcaatcgg
     5701 ctacggttat cccgccggcg aaaatgttat cactcaagga atccctgagc catacgcgaa
     5761 gcgaaccgtg gaaggagatt tgggcattcg tttcaacgcc gccacacttc tggggcaggt
     5821 cggtccggat cgcttcgagg tggagtggcg ctcgatgttt cctcatttac aagtctcgcg
     5881 cgatgagatc ggccgctaca tcaaccatat cagcgaaaac accgctcgtg ttcccgaaga
     5941 aaaatggcat agggctgcgg acgccgtgtt ggcgcgcgtc gccgcagata ttgctctgga
     6001 gcgtcatgtg ggcaaacgag aaagggttgt gacatctgcc ggcgaggcct gggtgcacta
     6061 cggcaaagac ttgcgcaata cgccgcgcct cctcgcaacc ggcggtgtct tcgtgcacaa
     6121 tccctacgct tcaatgatct tgtccggctc cgcccaagat cagactgacg tgactgtcct
     6181 acgtccaaag cagccgcagc tttatttgga ttcctcctac ctactttacg cggttggatt
     6241 gcttgggggg gattacccgg atgtggcggt gcggctcttt aagacgtata tgacgccggt
     6301 tgcgcaatcg tgaagttaat gtttagcagt gcgaaggcga aaacgtgaac atggccagac
     6361 ccggatttgt aaagcttcga ttagggctgg tgcccagtgg cagcagttgc caatacaatg
     6421 tctgtggatt ggcgctaggt tgtgttaatt gacaccggaa acgacactgc tccccttcgg
     6481 tggccgcttg cctttctgag gccgttcgaa aaaagcatct tcgcggtgaa cataaccgcg
     6541 aatcgtcttg ccttcgaaaa ccgcctgtac ctgaagatag tctcctttgg cgctgatcac
     6601 cttgatacgt gtagacggtt cgagcatagc gatgattttg gcctctgccc gagcgctacc
     6661 gcggacaaac gatggctgtc cgacgacttc gaaggttccc aaacgcgagt ctctcttctt
     6721 cgccgagctt tcttgtggtt ggggttgttc cgggttgggc gcgggcgttt gcggagcaac
     6781 aggatttttc ggaagatctt ccggcttggc ttgcaccgga gttacgcttt ggcgcaggtt
     6841 taaagaaagc tctggctctc ttctttggac cgtcactgaa cgattttgat acagaggaag
     6901 cggtggcaga ctgcgatttc ccaaggaggg ctcttgcccg aggtatcctt tgatctgctg
     6961 agaatacagg gcgccaccgg cgccgccaag gccaatcacg gccaagacca tgcccgtggg
     7021 tacccaagcg aaacttctgc tgcgcccgtg ttgttccggg aaagcgatcg gaccggacga
     7081 tgtagccggg ttggcttgaa gattagcgtc acgtatgtcc gctgccgcgt aagcgaggcg
     7141 gcggtttgac tcggaaaaat ttgctaatcc aagatcgcgc gcaacctctt gaactatttc
     7201 gcttgtgact gtttttttgc catcggcgta ggcaagaagt agtgcgttat cgcagataat
     7261 gttaatcaag cgaggaatac cgccggaaaa caagctgata tccgcaagta ccgccggatc
     7321 gaacaagtcg ttaccgcgaa atcctgcccc gcgaactctg aagagaatat aacgagtgac
     7381 ctcgtcattg gtgagaggag taaggcggct atggatgacg atgcgttgct tcaaatggtg
     7441 tagtttcggt tgattcagtc gatattgaag ctcaggttga ccggtcaata cgatctgtag
     7501 taatttttcg ccgccatatt gaaaatttga aaagaaacga atcgtttcga atgtctcctc
     7561 ggtcaagttt tgagcttcat cgatcaatag cgcgacgatg cggcgcctgc gaaattgtgc
     7621 aatcaagtag tggcgcagct gctcgaggct tgaaactcta tcttggcgat cgctcaccag
     7681 tcccagctca cgggaaacta gctggatcag ctcgcttgaa ttgagttgat cgtatgagat
     7741 gaagataacc tgaatcgtcg agcccaagtc gcgaatgatt ttatcgagca gcgtggtctt
     7801 tcctgtgccg acatcgccag tcagcacgat taaacctttt ctccattcaa tgccgtaacg
     7861 cagattggca aaggcctcgc gatacagttc gttatcgtaa aagattcgag aatccggtgt
     7921 ggcgctaaac ggtggcgcta ggaagccaaa gaattcgttg tacataaaag gcgatggcag
     7981 cctgaactac agtgacttaa gacgggcgct cagcacgctt gcggtgatat tcggagctgg
     8041 tcgttgacac ttcagcgatc tatctcgccg cacggcagca actaatctct tcgtcaggtt
     8101 atgctgaact caatgattca aaagcgtaga tcattctata atctcggctt caatcgttac
     8161 gtaataaaac aggaagacat tgttcgcaca ctgttgcgac ggttgtcaag aatgtttttg
     8221 gatgttcgct ctgttattaa aaccttgaac tggattggca gtcgtatctt ggaggcggga
     8281 tttgcgacga caatctatag acgattcgca agcctagcgt ctgcccggac cgacttttag
     8341 aagtcgacca tcccggctgt cggtcaggag ataaacaaat ccattgggtc cttgccgtac
     8401 gtcgcgaatc cgttcattga gttcgctaag gtaacgctct tctcgcgcaa tccggccatc
     8461 cttcatcacc agacgaacca gcaatcccgg attcagtgag cccacgagca tgcttccttt
     8521 ccagtcggga aatatatccc cggtatagaa gatcatcccg gatggagcga taaccggatc
     8581 ccaatagtag acaggctgtt ccatgccggg ttttgctgtg ccttcaccga tcttcgctcc
     8641 tgaatagtca acgccgtatg tgatgatcgg ccaaccatag tttttgccgg cctgcggatg
     8701 atttagctcg tcgccgccgc gagcgccgtg ctcgacagtc caaagctgac ccgtctgcgg
     8761 atcgattgca gcgctttgga tattccggtg gccgtatgac caaatctctg gcttcgcccc
     8821 tttacgatcg acgagtgggt tgtctttggg taccgatccg tcaggattga tacgcacgat
     8881 tttgccgatg ccagaggaca gatcctgcgc ctgatcgcgg taggcaaaac gatcgccctg
     8941 agttacgaaa aggcttccat cgttagcaaa aaccaggcgc gacccgaagt gattcgggcc
     9001 tgagacctta ggttgctggc gatagatcac ctccacgtct tttaggcctg tttcgccaag
     9061 acgtccgcgg gcaacgctag tgcccgccgc gccattttct cccggttccg aataagacac
     9121 gtaaaccaaa cgattgtcac ggaaccgtgg atcgaccgaa acatcgagaa gaccaccttg
     9181 cccgcgcgcc agcactcttg gtattccctc cggtggtttg gaaagaatcc cggcatgatc
     9241 cactttgcgc atccgcccgg gacgttccgt aataagcatg aatccgtcag gaagaaatgt
     9301 caggccccaa ggatgctcaa gcgcttttgc gacggtctca acatataact tggcgtttaa
     9361 cggcgcgggt gtcggcgagc gcggcgcctg gctttgcgcc ggtgcgatta acccg
//
LOCUS       DASUTG010000855         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_428766, whole genome shotgun sequence.
ACCESSION   DASUTG010000855 DASUTG010000000
VERSION     DASUTG010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428766"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VF089_19940"
     CDS             complement(<1..962)
                     /locus_tag="VF089_19940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6176286.1"
                     /translation="MKVNEQKLNEFMEKAISDLAAGYGGVMVALGDKLGLYKAMAGQG
                     PLSSTEVAKLAGCAERYVREWLNSQAAAGYVVYHPSSETYELTPEQAMVLANEDSPVF
                     LPPAWAVPASMWFDEDKSIEAFRTGKGIGWDEHNPRLYHGVAAFYRNAYQGQLVSDWL
                     PALQNGMEKLNAGAKVADVGCGHGHSTIIMAEAFPGSRFWGFDIHPESIDAARGNAKK
                     RGAEDRVTFEVADAKNYPAQEFDLICFFDCLHDMGDPLGAARHARESLSPNGSVMLVE
                     PYANDRVEDNLNTIGRLYYSASTTLCCAHSLSEEGMALGAQAGE"
     gene            1081..2130
                     /locus_tag="VF089_19945"
     CDS             1081..2130
                     /locus_tag="VF089_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6176287.1"
                     /translation="MNGGKKSIGVSLLALPEVMASTLIGLYDVLNSFTLLSTYNDALP
                     SDPPFTVEIVAPNPGLTHVSSGMPIPAGRSIDDIDSTDVVIVPSVIVARGEWLKGRYP
                     NIVRWLTEMHANGAQLCSTCSGVLLLAETGLLEGKEATIHWIYAPTFQRNFPGIHLRV
                     EKALVAIGDNNQFIMSGASTAWHDLILCLISQHAGPTAAQAIAKFFAFQWHADGLSPY
                     VMFEPVMDHGDAVIQETQKWLNKHFSVPSPIEQAVRRSGIAERTFKRRFKSATGHTPI
                     EYVQRLRVDEAKRRLERTTAPIDEIGWQVGYEDPTFFRRLFKRLTGVTASAYRRRFRI
                     PDSAPLAKMKDEVRY"
     gene            complement(2191..2727)
                     /locus_tag="VF089_19950"
     CDS             complement(2191..2727)
                     /locus_tag="VF089_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6176288.1"
                     /translation="MESRYLIFLACGALTFLGAACAFNSAQETTPADWEQTSYVERGN
                     YLVNYLGHCVGCHTPLGPDGQSNMSLYLSGVPAKYAGTKVGRPQVAGFPGPRGARFYA
                     ANLTPDPETGLGRWSEEQFVRTFKHGVRPDGTKYATSPMEWNIYANMKEEDVRAIYRY
                     LRTIKPIPNKVPANIPPK"
     gene            complement(2740..3246)
                     /locus_tag="VF089_19955"
     CDS             complement(2740..3246)
                     /locus_tag="VF089_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176289.1"
                     /translation="MNWISTLLMIVATSFLVLSPPSARGESEAEIIARGRYIATAIQG
                     CGCHTREKPDGSKDENWHYAGSPNPAPPAGPPANAGWSSPRWKKIYASNITPDPETGI
                     GKWTEAQFIRAMKTGITPDGRVLDAQMPWDAFQKITDRDLKSLWAYLRTIKPIKNTPP
                     ERIPAEQK"
     gene            3316..3696
                     /locus_tag="VF089_19960"
     CDS             3316..3696
                     /locus_tag="VF089_19960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6176290.1"
                     /translation="MIFRFSQAKNNKLRILIVSGVLFLCCSDAFADIEQGKRIYRENC
                     APCHGESGKGDGVGARSLPVRPADHTNPAVMNSRSDAFLRDVIAKGGKAMGLSAFMPA
                     WQGIFKDKEIEDLLVYIRSLAAAK"
     gene            4228..>4410
                     /locus_tag="VF089_19965"
     CDS             4228..>4410
                     /locus_tag="VF089_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176291.1"
                     /translation="MDGTITWMLVLAGAAVALLGAFLVVSEKELRKKRREVASLAAKL
                     SRPNTDLVPGDYQNNLA"
BASE COUNT          953 a         1233 c         1179 g         1045 t
ORIGIN      
        1 ttctcgcccg cctgcgcccc gagcgccatc ccttcttcgg acaaggagtg ggcgcagcag
       61 agcgtcgtcg acgcggaata gtaaaggcgc ccgatagtat ttagattatc ttcgacccgg
      121 tcattggcgt acggttcaac cagcatgaca gagccgtttg gcgacagcga ttcgcgtgcg
      181 tggcgcgccg cgcccaatgg atctcccatg tcgtgcaaac aatcgaagaa acagatcaag
      241 tcgaactctt gcgcgggata gttcttcgcg tccgcaacct cgaacgtcac acgatcctcg
      301 gccccgcgct ttttagcgtt gcctcgcgcg gcatcgatcg actccgggtg aatgtcgaag
      361 ccccagaatc gagatccggg aaacgcttcg gccatgatga ttgtcgagtg tccgtgaccg
      421 cagccaacat cggcgacctt ggccccggca tttagcttct ccatgccgtt ttgcagcgcg
      481 ggtagccaat ccgaaactaa ctggccttgg tatgcgtttc ggtaaaaggc agcgacaccg
      541 tggtacaggc gcggattgtg ctcgtcccac ccgatgcctt tacccgtgcg gaacgcctcg
      601 atcgatttgt cttcatcgaa ccacatagac gccggcaccg cccaggctgg cggtaaaaaa
      661 accgggctgt cctcgttagc gagcaccatc gcctgctcgg gtgtgagctc gtaggtctcg
      721 ctggatgggt gataaacgac atagcccgcg gctgcctggc tgttgagcca ttcgcgcaca
      781 taccgctcag cgcaaccagc cagtttggcg acctccgtgg agcttaaagg accctgtccg
      841 gccattgcct tgtacaagcc gagtttgtct cccaaggcga ccatcactcc gccataaccc
      901 gccgccagat cactgatcgc cttttccata aactcattta gcttttgctc gttgaccttc
      961 atcgttcttt cctcctaagg tagatttgct tttacgccag tcaaaataat ctctcgagcc
     1021 tgtctgtaga agagcgaaaa cggtcaaaag aggtccgttt atgccagagg ggataaaaaa
     1081 atgaacggcg gaaaaaaatc cattggcgtc agtctcttgg ctcttcccga agtcatggcc
     1141 tcgaccttaa tcggactcta cgacgtgttg aattcgttca ctctactttc cacttacaat
     1201 gatgcgttac cgagtgaccc gccgttcacc gtggaaatcg tcgcgcccaa tcccggcctg
     1261 acccacgttt ccagcgggat gccgatcccg gccgggcgat ccatcgatga tatcgattcc
     1321 acagatgtgg tcattgtacc gtcggtcatc gtggcgcgcg gcgagtggct taaaggcaga
     1381 tatccaaaca tcgtccgttg gctaaccgaa atgcatgcca acggcgcaca gttgtgttcg
     1441 acgtgctccg gcgtattact gttggcggaa actggtttat tggagggcaa agaagccacc
     1501 attcactgga tttacgcgcc gacttttcag agaaactttc ccggtattca tttgcgcgta
     1561 gagaaagcct tggttgcgat cggcgacaat aatcaattca tcatgtccgg cgcgtcgacg
     1621 gcgtggcacg atttgattct ctgtttgatc tcccagcatg ccggtcctac agccgcccaa
     1681 gcgatcgcca aatttttcgc attccaatgg catgccgacg gcttgtctcc ctatgtcatg
     1741 tttgaacccg tgatggatca tggcgacgct gtcattcaag aaactcagaa atggctcaac
     1801 aagcatttct cggtgccgag cccgatcgag caagcagtgc gccgatccgg catcgctgag
     1861 cgcaccttca aacggcgctt taaaagcgca accggccaca cgcctattga atatgtccag
     1921 cgcttgcgtg tggatgaagc aaagcggcgc ttggaaagaa caaccgcgcc catcgatgaa
     1981 attggctggc aggtcggcta cgaagacccg accttttttc gccgcttatt caagcgtctc
     2041 acaggggtta ccgcgagcgc ctatagacgc aggttcagga tcccggactc tgccccgctc
     2101 gcgaagatga aggacgaagt tagatattaa ccgcttgatt ccttcttgag gatagaccgc
     2161 ggctctttcc agttgtcgac cagccgcacg ctatttcggc gggatgttcg ccggcacttt
     2221 attgggtatc ggcttgatgg ttctaagata acgatagatg gcgcgaacat cctcttcctt
     2281 catattggca tagatgttcc actccatcgg cgaggtcgcg tacttggtgc cgtcgggcct
     2341 aacgccgtgt ttgaatgtcc ggacgaactg ctcctcgctc cagcgcccga gccccgtctc
     2401 cgggtccgga gttagattcg cagcgtaaaa acgcgccccc cttggtcctg gaaagccggc
     2461 cacctgggga cgacctactt tcgtgccggc atattttgcc ggcacgccgg atagataaag
     2521 gctcatgttt gattggccgt cgggacccaa cggagtgtga cagccgacgc agtggccgag
     2581 atagttcacc agatagttgc ctcgttcgac gtaacttgtt tgctcccaat cggcaggggt
     2641 cgtttcctga gcggagttaa aggcgcacgc cgcaccaaga aaagtcaatg cgccgcaagc
     2701 aagaaagatt aaataccgcg attccatagt ttgctccggt tatttttgtt cggctgggat
     2761 tctttcgggc ggcgtattct tgatcggctt gatcgtcctg agatacgccc atagcgactt
     2821 caggtcgcga tcggtgatct tttgaaaggc atcccacggc atctgggcat cgagcactcg
     2881 tccgtccggc gtaatgccgg ttttcatggc tctaataaac tgagcttcgg tccatttgcc
     2941 gatgccggtt tcaggatcgg gcgtgatgtt actcgcataa atcttcttcc agcggggcga
     3001 ggaccacccc gcgtttgccg gcggaccggc aggcggcgcg ggattgggcg aaccggcata
     3061 gtgccaattc tcatccttgc tgccatcggg tttttcgcgc gtgtgacagc cgcagccttg
     3121 gatcgcagtg gcgatgtagc ggccgcgcgc gattatttcc gcttcgctct cgccgcgcgc
     3181 ggatggcggc gacaacacga ggaaagatgt tgcaacgatc atcaaaagag tagatatcca
     3241 attcatctat cgacctttcc acaaacctga tcatcgcgat gtagcattgt gtcaaagatg
     3301 tttgcaactg tattaatgat ttttagattc agccaggcaa agaataacaa gctacgaatt
     3361 ctgattgtct ccggcgttct gttcctatgc tgctcagatg ccttcgctga tatcgaacag
     3421 ggcaaacgga tctaccgtga gaactgcgct ccctgccacg gtgagtcggg caaaggcgat
     3481 ggagttggcg cgcgatcgct gccggtgcgt ccggccgatc ataccaaccc tgcggtgatg
     3541 aattcgcgca gtgacgcttt tctgcgcgat gtcatagcca aagggggtaa ggcgatgggg
     3601 ctatccgctt tcatgccggc atggcaagga atcttcaaag ataaagagat tgaagatctc
     3661 cttgtttaca tccgtagctt ggcggctgcc aagtgacatc gtctacactc gtcagaggat
     3721 tgaattagta ttttcaatat gaattcctgc cggcgataca ccttgtcgtt cttgatcatt
     3781 ggactatctc cttccggctc ggagcgatgt ctctcatcct taaaccggtg actcaccggc
     3841 ttcgttagct ccaggagccc ggttttcagc cgtttacaat tttcgccagg attcacgatt
     3901 ttgtttatgg gccgacgaat tccagcggca ttaagctcgc ttgcctgctc gatgagtcgc
     3961 gcagtatcag cccttttaag gatttggtgc ggcaatcagg agaattggca catatttggc
     4021 gttatcggct ccggcgaatg aagtctggcg ccagccgaaa cttggtaagc ggttgagaaa
     4081 aactcaactg aaccggtaaa gtagaatgat tctccacacc tcctctaacg aataacaacg
     4141 cagacaggcc gtgcatgatc gaagcatctg cacggcgcta aacttagcct ctatccggtt
     4201 cgatccagat gtaaatagga gaccacaatg gatggcacga ttacatggat gctcgttctc
     4261 gcgggcgctg cggtagccct gctgggcgcg tttctggttg tctctgaaaa ggaactgcga
     4321 aagaagcgcc gcgaagtggc aagtcttgcc gccaaacttt cacggcctaa cacggactta
     4381 gttcccggtg attaccaaaa caacctggcc
//
LOCUS       DASUTG010000856         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1913618, whole genome shotgun sequence.
ACCESSION   DASUTG010000856 DASUTG010000000
VERSION     DASUTG010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913618"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..689
                     /gene="tsaA"
                     /locus_tag="VF089_19970"
     CDS             204..689
                     /gene="tsaA"
                     /locus_tag="VF089_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX6176292.1"
                     /translation="MPEIIFKPIGQIKAQFSDADVRAERKDLEGDLEIYPQFEPALEG
                     IDGYSHLFLLAYFDRLRPDQIGPLQVKPRRLVNRGFKLGELPSLGVFALDSPTRPNPI
                     GLTLVRLLGRQGRILKVAGLDLFDGTPILDIKGYRPSYRADEFSVPDWHKMMQDPAGH
                     I"
     gene            796..1644
                     /locus_tag="VF089_19975"
     CDS             796..1644
                     /locus_tag="VF089_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6176293.1"
                     /translation="MDIQHFFDQATSTLTYVVADGSTGVVIDPVLDYDSKSARTSLKS
                     AEAVAQYIERRHLSIPYVIDTHAHADHLTAMPFFKKRYGALTVTGSRVGEVQRSFRDI
                     YNLGADFPVDGRQFDILLDEGQDLEVGSFRIRAMHTPGHTPAHMSWQVKDAVFVGDTL
                     FMPDYGTARCDFPGGSADQLYDSIQRLYALPDSTRLFMCHDYQPGGRELRFVTTVAEQ
                     KRSNVQLRQGTTKQEYVKIRTARDAKLEMPALILPAIQINIRAGEFPAPEANGRAYLK
                     IPLNLF"
     gene            1954..2760
                     /locus_tag="VF089_19980"
     CDS             1954..2760
                     /locus_tag="VF089_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6176294.1"
                     /translation="MDSYLQLLWLIPLGFFAGGYGTLIGAGGGFVLVPALLFIYPGEA
                     PETVTSISLAVVFFNALSGTLAYARSNRIHFKSGLIFAAATVPGAVAGALATTGMSRE
                     RFNVIFGLLLIAMATFLAINPGKKTAAGMAQAGSDIRKPLNLNGSLLIAGAGVSTVFG
                     FISSFFGIGGGFLYVPALVYLLRFPIHTATATSLFVLTITAFTGSVTHVAAGLFQHGF
                     RRAIMLSIGAIIGAQIAARFSQRIHGEWILRSLAAALGLVGLRLIVAVFE"
     gene            3170..>4512
                     /locus_tag="VF089_19985"
     CDS             3170..>4512
                     /locus_tag="VF089_19985"
                     /inference="COORDINATES: protein motif:HMM:NF027971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEX6176295.1"
                     /translation="MEQPKVKFLGAAGTVTGSKFLVSYNGTEILLDCGLFQGLKELRL
                     RNWSSFPVDVNGLRAVLLTHAHIDHSGYLPKLVKLGFRGNVYATSATCDLLKILLPDS
                     AHLQEEEAGYANRRGYSKHKPALPLYTVEDAERALELLRPVSYADEIELADGLSCRFT
                     RVGHILGAAAIRIGFDMAGGKRFLVDSGDLGRYGRPILKDPDSIEHADWLLLESTYGN
                     RTHPTNSEDELAKVIKKVAIAEGCLIIPSFAVGRTQEIIYSIRTLEDQGKIPAIPVHI
                     DSPMAINATDIYCAHSEEHDLDMELLNEAKLCPLCSKKFYIHRTAEDSKAINDMAGPL
                     IIISASGMVTGGRVLHHLKRRLPDAKTTVLLVGFQAEGTRGRTLQSGMKEVKMLGEII
                     PVRAEVKTIDGFSAHADRGEILRWLGSFRKRRKKLLSSMANRRRVRLWISSCVRS"
BASE COUNT         1009 a         1216 c         1234 g         1053 t
ORIGIN      
        1 gacaaacgcc acagcgtgac cggcgaagct ctcgacgagg aggcttatac gcgccacctt
       61 caagagacac tgccgacgca aaaagaccgg gaaaagttgc ggaagattac gcgagaggcg
      121 gactggatcg cggcaaacta aggagtgtta ccgttcgaaa ccgcggccag cggagcggag
      181 atatatagtg gagatattta tttatgcctg aaattatttt taagccgatc ggccagatca
      241 aagcacagtt ctccgacgct gatgttcgcg cggaacgcaa agatctcgag ggcgacctgg
      301 aaatttatcc gcagttcgag ccggctctgg aggggatcga cggctattcc catctttttc
      361 ttttggcgta ctttgacagg ctcagacccg atcagatcgg tcctttgcaa gtgaagccaa
      421 ggcggctggt caatagaggt ttcaaattgg gcgagcttcc ctcgctgggc gttttcgcgc
      481 tggattctcc gactcggcct aacccgatcg gactcacgct ggttcgcttg ctcggacggc
      541 agggtcgcat tctcaaagtg gcgggcttgg atcttttcga cggcacgccg attctcgaca
      601 tcaagggcta ccggccgagt tatcgcgccg atgagttcag tgttcccgat tggcacaaaa
      661 tgatgcagga cccagccggg catatttgag cccactgctt tcttcggcga acaaaggcga
      721 cgggctgatc atcgattttg ctgaaacgcc ttccagacga gagtctttgt tagaccgacg
      781 ctagaggaga tctgaatgga tatccaacac tttttcgatc aggcaacgtc aacgttaacc
      841 tacgtcgttg ccgatggcag caccggagtc gtgatcgacc ccgtgttgga ttacgactcg
      901 aagagcgcgc gtacatcttt aaagtccgca gaggctgtgg cgcaatacat cgagcgccgg
      961 cacctttcca tcccctatgt catcgacacc catgcgcacg cggatcacct gactgcgatg
     1021 ccgtttttta aaaaacgata cggagcactc acggtgaccg gcagccgggt cggcgaggtt
     1081 cagcgcagct ttcgcgatat ctacaatcta ggcgcggatt ttccggttga cggacggcag
     1141 ttcgatattt tgctcgatga aggccaggat ctcgaagtcg gttcgttccg catccgtgcc
     1201 atgcacacgc ctgggcacac accggcgcac atgtcctggc aagtcaaaga tgctgttttc
     1261 gtcggagata cgcttttcat gccggactat ggcaccgcgc gctgtgactt tcccggcgga
     1321 tccgccgatc agctttacga ttccatccag cgtctttatg cgctcccgga cagcacaagg
     1381 ctgtttatgt gtcacgacta tcaacccggc ggccgcgagc tgcgtttcgt tacgacggtc
     1441 gccgagcaga agcgctcgaa tgttcagctg cgtcagggca cgacgaaaca ggaatatgtc
     1501 aagattagaa cagcccgcga cgccaagctg gaaatgcccg cgctaattct gcctgcgatc
     1561 cagatcaata tccgcgcggg agaattcccc gcgccggagg caaatgggag ggcctatttg
     1621 aagatcccac tgaatttgtt ttgagagaaa ccttaaggtt cacgatttct caggcttcta
     1681 atccgtgaca cggcctgaag agcgatggcc tccactactt tcaagcgtaa agagctcgtt
     1741 taccaaagcc ccgtgttctg gaactgaagt tcagaagtag tcagagctgt tggcaacgtc
     1801 attgccgcgc taagcagatc gaaggtcttc aaattcgtat attgcgggtc gcgcaagcta
     1861 tcgccagctc gcttgagatg acattccttt aacttcaggt acagaccgtt agcgagtttt
     1921 acacggtgat aacaatataa acgataacat cccgtggatt cttacctaca actcctctgg
     1981 ctcataccac tgggtttttt tgccggcggc tacggtactc tcattggcgc cggcggtgga
     2041 tttgttctgg tccccgcgtt gttgttcatc tatcccgggg aagcgccgga aacggttacg
     2101 agcatttcgc tggccgtggt tttctttaat gcgctttccg ggacgctcgc ctacgctcga
     2161 tccaaccgaa tccacttcaa atcagggttg atctttgccg ccgcgacggt acccggggcg
     2221 gtggctgggg cgctcgcaac cacgggcatg agccgggagc gcttcaatgt gatctttggc
     2281 ctgctactga tcgcaatggc cactttttta gcgattaacc cgggcaagaa aaccgcggcc
     2341 gggatggccc aagccggaag cgatataaga aagcctctga atctgaatgg gtctctcctg
     2401 atcgccggcg ctggtgtaag caccgtgttc ggttttatct ctagcttctt cggaatcggc
     2461 ggcgggtttc tgtacgtccc tgcgctggtt tatctgctac gcttcccaat tcataccgcc
     2521 accgcgactt cgctgtttgt tctgacgatc accgcgttta caggcagcgt gacacatgtt
     2581 gccgcgggcc tctttcagca cgggtttcgc cgcgccataa tgctttccat cggcgccatt
     2641 atcggcgcgc agatagccgc gcgattttcc cagcgcattc acggcgaatg gatactgcgc
     2701 agcctagccg cagcattggg attggtaggg ctgcggctca tcgtggccgt ttttgaatga
     2761 atcctccgcc agtcatcgag cctgttcttt cacaagcttg caaaaacctc cgccccattc
     2821 cgcaggcctg cgaaaatata tgccgattca acagcaaact ctaaactgtc cctggtattg
     2881 gcgttgcgta gtattgttca cgtctgacag agtggcaaaa cgtagagaac accactgact
     2941 gaacgatgga tgttatcggc ggtgtcggct tgagccaaag atcgcgcaaa aatcactttc
     3001 cggcgcggct cgatggcttg ccccagaatc gccgctgtcc atgcagatag tctgtacaag
     3061 cgagagaggg gccgtaggat ggaagtgatc gggtgacatg atgtcgccgg acttgcgcgc
     3121 ttagcactat cgtactctca ccagtgccgg ctaaggaacg gaaacaggaa tggagcaacc
     3181 aaaagttaaa ttcttgggcg ccgcgggtac cgttaccgga tcgaagtttc tcgtctcgta
     3241 taatggaacc gagattctgt tggattgcgg tttgtttcag ggattgaaag aactgcgact
     3301 gcgtaattgg tcgtctttcc cggtggatgt taacgggctt cgcgcggtcc tcctaactca
     3361 cgcccacatc gaccactccg gttatctccc gaagctcgtc aaactggggt ttcgcgggaa
     3421 tgtttatgct acctccgcaa cctgcgatct gctgaaaatt ctcttgccgg attctgctca
     3481 tcttcaggag gaagaagccg gttacgctaa tcggcgcggc tattccaagc acaagccggc
     3541 tttgccgctt tacactgttg aggatgccga gcgtgctctg gagctcctgc ggccggtaag
     3601 ttacgccgac gagatcgagc tcgccgacgg gttgtcatgt cgttttaccc gcgtcggcca
     3661 tatcctgggc gcggctgcta tccgcatcgg ttttgacatg gctggtggaa agagattttt
     3721 agtcgattca ggagatctag gccggtatgg aaggccaatc ttaaaagatc ccgactcgat
     3781 cgaacatgcc gattggctgc tgttggaatc gacttatgga aaccgaacgc atccgacaaa
     3841 ctcggaagac gaactggcga aagtcatcaa aaaggtagcg atcgccgagg gatgcttgat
     3901 tatcccgtca tttgccgtgg gaaggaccca ggagattatt tacagcatcc gtacactaga
     3961 ggatcaaggg aaaataccgg cgattccggt gcacattgac agccctatgg cgataaacgc
     4021 aacggatatt tactgtgctc attctgaaga gcacgatctc gatatggagc tcctcaacga
     4081 ggcgaagctc tgtccccttt gttccaagaa gttttacata cacagaactg ccgaagattc
     4141 taaagccatc aacgacatgg ccggaccttt aataatcatt tccgcaagcg gaatggtcac
     4201 cggcggccgt gttctccatc atttaaagcg ccggctgccc gatgccaaaa caaccgtgtt
     4261 gctggttggt tttcaggcgg aaggaacccg cggcagaacg cttcagagcg ggatgaagga
     4321 agtaaaaatg ctcggtgaaa tcatccccgt cagagccgaa gtgaaaacca tcgacggttt
     4381 ttccgcccat gccgaccgag gcgaaattct gcgctggcta ggatctttca gaaagcgccg
     4441 aaaaaaactt ttgtcatcca tggcgaaccg ccggcgagta aggctctgga tctcctcctg
     4501 cgtgaggagt tg
//
LOCUS       DASUTG010000857         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1763369, whole genome shotgun sequence.
ACCESSION   DASUTG010000857 DASUTG010000000
VERSION     DASUTG010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763369"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1855)
                     /locus_tag="VF089_19990"
     CDS             complement(74..1855)
                     /locus_tag="VF089_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176296.1"
                     /translation="MKHTVRSYLKPYWPALLLALGQVFLISAFELIKPWPLKIIIDNI
                     LSDNPLPFGLSLNWSGDTLLIVACAGLILVYLLLGGLRVLSDYTTIRIGQGMVNDLRR
                     DLYSRIQRLSLSFHTRQQVGDLLYRITADTMGIQTLTMNGFFAVLSASVLLAGMFVVM
                     ISLDLYLTLLALVVCPGLLAAIALLNKRMSSAAAYARDKESSVYSLVQRTLSGIRVVQ
                     AFTKEEDEERRFVKASTESLAAELRLYNLQNFYYVVVDVMIAAGTAAVLWVGTRHVLS
                     GQLTVGDLVVFTSYLASLYAPLNSIFQTYGSVQGARIGVRRAFEILDSEHALPEGHRT
                     LSQRRAKGQITFENVTFGYSSSQPVLKSVNLHIAPGKRVAVVGPTGAGKSTLVSLLPR
                     FYDPQAGCVKIDGVDIREFQLKSLRRQIAMVLQPPVLFPVTIRKNIAYGRPEASLDEV
                     IAAAKVAQVHDFIEQLPKKYDTIVGEQASNLSEGQKLRLTIARGVLLDAPILILDEPT
                     SSVDPETESLIMEGLEHLMEGRTTFIIAHRLSTVRQADVILVVRDGRIVEQGGFTELL
                     RRRGVFASMYRTQFGLRENERHFHVIG"
     gene            complement(1866..>2538)
                     /locus_tag="VF089_19995"
     CDS             complement(1866..>2538)
                     /locus_tag="VF089_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX6176297.1"
                     /translation="VRCLTSALRARVPRAYRGAAATLLITFLIYVGPLFRTLERYRWR
                     IRRLREVKPVECNGPRQSPRISLRERAFYLSYWNEAGQEKESLLGAAMDFLLPRKYLI
                     AMDQGWSQWDLEICQGPWAKAQVKTAVENHGGSKRLLRVRCALRMSRVSLICLSLYAV
                     LTTTAAFLATPQIALATLGIGLIHGSAILYQKFRLGHVLYHVMESLAHKLEFVPLKKS
                     SQEAL"
BASE COUNT          605 a          702 c          717 g          514 t
ORIGIN      
        1 cgatgatacg acggcgcgtc atagctgatc ctattccgtc aaggcgaaga gttttggcaa
       61 gcggccggtt cgcttaaccg atcacatgaa agtgccgctc gttctcgcgc agaccaaatt
      121 gggttcggta cattgacgca aagacaccgc gccggcgcag caactccgta aatccaccct
      181 gctctacaat ccgaccgtcg cgaacgacga gtatcacatc ggcctggcgc accgtggaga
      241 gacggtgcgc gataatgaaa gtggtccgtc cctccatcag gtgctccaga ccttccatga
      301 ttagcgactc agtttccgga tcgaccgatg acgtcggctc gtcgagaatc agaatcggag
      361 catcgagcaa aacaccgcgt gcgatcgtaa ggcgcaattt ctgcccttcc gataggttcg
      421 atgcttgttc gcccacaatc gtgtcgtact ttttcggaag ctgctcgatg aaatcgtgca
      481 cctgtgccac tttcgcagca gcgatcactt cgtccagaga tgcttcgggc cggccatagg
      541 cgatgttttt ccgaatggtg accggaaaga gcaccggcgg ctgaagcacc atcgcgattt
      601 gccgccgcaa cgacttcagc tgaaactctc ggatatcaac gccgtcgatt ttgacgcagc
      661 ctgcctgcgg atcatagaag cgcggcagta gactcaccaa ggttgatttt ccggcgccgg
      721 tggggccgac gaccgcgacc ctctttcccg gcgcgatatg caaatttacg ctcttcagca
      781 ctggttggga ggagctatag ccaaaggtaa cgttctcgaa agtgatctgg cccttggcgc
      841 gccgctgtga gagggtgcga tggccttctg ggagtgcgtg ttccgaatcg aggatctcga
      901 aagcgcgccg gaccccgatc ctggcacctt gaaccgaccc ataggtctga aagatactgt
      961 tgaggggagc gtaaagcgac gccagatacg aggtgaaaac taccaggtct ccgactgtta
     1021 attggccgga caaaacgtgg cgcgtgccga cccataacac agccgccgtt ccagccgcga
     1081 tcatgacgtc cacgacgaca taatagaaat tttgtaaatt atagagccgc aactcggctg
     1141 ccagactttc ggtgctcgct tttacgaagc gccgctcctc gtcttcttct ttggtaaaag
     1201 cttggacaac gcggattccc gaaagcgtac gctggacgag ggaatagact gagctttctt
     1261 tatcccgcgc gtacgccgcg gcagaggaca ttctcttatt taaaagtgcg atggcggcga
     1321 gtagaccggg acaaacaacg agggccaata acgtgaggta gagatcaagc gaaatcatca
     1381 cgacaaacat tccggcgagc aggaccgaag ccgaaagtac ggcaaaaaag ccattcatgg
     1441 tgagcgtctg aatgcccatg gtgtcggccg tgattcgata caggaggtca ccgacctgct
     1501 gccgagtgtg aaacgagagc gacaggcgtt ggattcgact atatagatcg cgccgaagat
     1561 cgttaaccat gccctgtccg atgcgtatcg tggtgtagtc actcaggacg cgtaaaccgc
     1621 cgagcagaag gtaaaccagg atcaagccgg cgcacgcaac tatgagcagg gtgtctccag
     1681 accagttgag agaaagcccg aacgggaggg gattgtcgct caatatattg tcgatgatga
     1741 tcttgagcgg ccacggcttg ataagctcga aggcgctgat cagaaaaacc tgaccgagcg
     1801 cgagaaggag agcgggccaa tagggcttta agtaggaacg gaccgtgtgc ttcatggcga
     1861 ttttgtcaca atgcctcttg cgatgatttt ttgagcggca caaattctag tttgtgcgcc
     1921 aaagattcca tgacgtgata gagtacgtgg ccgaggcgga atttttgata caggatcgcg
     1981 ctcccgtgaa ttagcccgat accaagtgtc gccagggcaa tttgcggcgt tgctaagaaa
     2041 gcggcggtcg tagtcaatac cgcataaagg gacaggcaga tgagcgaaac cctcgacatc
     2101 ctcagagcgc agcgcacacg taaaagccgc tttgagccgc catgattctc taccgccgtc
     2161 ttcacttgag cttttgccca gggaccctgg caaatctcca gatcccattg gctccatcct
     2221 tgatccatcg cgatgagata ctttcgcggc agaagaaagt ccattgccgc gccgagcagg
     2281 ctctcctttt cctgtcccgc ctcgttccag tacgaaagat aaaacgcgcg ttcgcgcagc
     2341 gagatgcgcg gcgactgccg tggcccgtta cactcaaccg gtttgacctc gcgcagcctg
     2401 cgaatgcgcc agcgatagcg ctcgagggtg cgaaaaagcg gccccacata aataagaaat
     2461 gttatcagca gagtcgccgc ggcgccccgg taagctcgcg ggacgcgagc tcgcagcgca
     2521 cttgtcaggc accgcacg
//
LOCUS       DASUTG010000858         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1613657, whole genome shotgun sequence.
ACCESSION   DASUTG010000858 DASUTG010000000
VERSION     DASUTG010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613657"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VF089_20000"
     CDS             <1..901
                     /locus_tag="VF089_20000"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176298.1"
                     /translation="GAYVLEIPAGKSLNPQRHLYEDMLYVLKGRGATTIWNDKGAKQT
                     FEWQEGSLFALPLNSWYQHFNGQGDEPARFFSVNSMAITFNLFHNDDFIFNTPRDFVD
                     RFDGDPEYFSGKGKAHPGRVWDTNFIADARNFKLEEWKERGAAGKNVMLEMANGSMAA
                     HISEFPVGTYKKAHRHGPGFNVMVIKGHGFSLFWREGEQPKRYDWQDGSVFTPPAMMF
                     HQHFNTGATGARYLPPRLGGIKYSLGEGFGDITKVDKDVKAGGNQIEYYDEDPNIRKM
                     FEEELAKSGTTTKMNPDFYKKPA"
     gene            1016..1606
                     /gene="plsY"
                     /locus_tag="VF089_20005"
     CDS             1016..1606
                     /gene="plsY"
                     /locus_tag="VF089_20005"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX6176299.1"
                     /translation="MEILLVLLGYLLGSVPVGFILGLRSGIDVRETGSGNVGATNVAR
                     VVGKRQGILTLVADTAKGFLPVILAIQLGASRAATTLVGAAAFLGHLFPIFLKFKGGK
                     GVATAFGVFLALAPTATLVLVARFGVTFLTSRIVSLSSILSAVAAPILLWLFSYPRLV
                     IGMAAFIALAITWRHQSNIRRLMNGSEPRFGSANSQ"
     gene            complement(1593..2300)
                     /locus_tag="VF089_20010"
     CDS             complement(1593..2300)
                     /locus_tag="VF089_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6176300.1"
                     /translation="MNNSPIFTAAVIALSMVAAGCSGGTLSTREKGAGIGALGGAATG
                     AIVGAAVGRPGVGAAVGGALGLGTGALIGDQLQGQDIKQAEQQKAIDQQRAEIAKNQA
                     LIDELKKRNIEARETSRGVTVNLPNVNFQFDSADLTPDGRDRVNQIANILQREAPNRK
                     ITVEGHASRESAAQESYNQRLSERRASTVAQSLERNGINSGRISSQGLGTRAPIASND
                     SEEGRRQNRRVEVIIEN"
     gene            complement(2338..2673)
                     /locus_tag="VF089_20015"
     CDS             complement(2338..2673)
                     /locus_tag="VF089_20015"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6176301.1"
                     /translation="MRRIVTIIVGFGLLSATGCSSGGLTTREKGAGIGALGGAAVGGI
                     IGAAVGRPGAGAAIGGALGLGSGALVGDQLQGQEQKQSEQQRQIDANQAELERQRRDL
                     EGLKKTGEY"
     gene            2885..3985
                     /locus_tag="VF089_20020"
     CDS             2885..3985
                     /locus_tag="VF089_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6176302.1"
                     /translation="METLSQERILGVLKKVQDPELHRDIVSLGMVKNLVLKDGKVSFT
                     VELTTPACPLRETIETDCKKALAEVPGIKGLEISFGAQVRGSKAGEGQTDLLPSVKNV
                     VLVAAGKGGVGKSTVAANLAVALKKHGAATGLLDADIYGPSVPIIMGVKGEPAKVEVN
                     GAFKIAPTIAHGVPVMSIGFFLDPEQAVIWRGPMLGKALHQLMADVHWGELDYLVVDM
                     PPGTGDVQITFSQQLKVSGALLVATPQDVALADVIRAKTMFDKVMIPIVGVVENMSYF
                     ICDGCNKKHEIFSRGGALRAAERFNIPYLGEIPITPALREGGDKGLPILIQDPNSEVS
                     KVFLEIAAKLAGQLSIASERAKQTQGLKIVSS"
     gene            4032..4331
                     /locus_tag="VF089_20025"
     CDS             4032..4331
                     /locus_tag="VF089_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX6176303.1"
                     /translation="MVTKEQVYEALHECYDPEIPVNIVDLGLVYDVEVNNDNVAVKMT
                     LTAPGCGMGTMIAADAKEKILALEGVNDATVDLVWDPPWDPSRISDEAKQRLGIG"
BASE COUNT          982 a         1153 c         1214 g         1087 t
ORIGIN      
        1 cggagcctat gtgttggaaa tccccgcggg aaagagcctc aacccgcaga ggcatctcta
       61 cgaagacatg ctttatgttc tcaaaggtcg tggtgcaacg accatttgga atgacaaggg
      121 agccaagcaa acgtttgaat ggcaggaggg cagccttttc gctttgccgc ttaattcctg
      181 gtaccagcac ttcaacggcc agggcgacga gccagcgcgg tttttctcgg tcaacagcat
      241 ggccatcacc tttaaccttt ttcataacga cgactttatc ttcaatacgc ctcgtgactt
      301 cgtcgatcgt ttcgacggcg atcccgaata tttcagcggg aagggcaaag ctcatccggg
      361 ccgggtttgg gataccaact ttattgccga tgcccgcaac ttcaaacttg aagagtggaa
      421 ggagcgcggc gcggcgggaa aaaatgtcat gctcgagatg gccaatggct cgatggctgc
      481 gcatatttcc gaatttcccg tgggcaccta taagaaagcg catcggcacg gacctggttt
      541 caatgtcatg gtcatcaaag gtcatggctt ctcgctgttc tggagagaag gagaacagcc
      601 caaaagatat gattggcagg acggcagcgt ttttacgccg cccgcgatga tgttccacca
      661 gcattttaac accggcgcca cgggggcgcg ttatctgccg ccgcgtttag gcgggatcaa
      721 gtatagcttg ggcgaaggct tcggcgacat caccaaggtt gataaagacg ttaaggctgg
      781 tggcaatcag attgagtatt atgatgagga tccgaacatc cgaaaaatgt tcgaggagga
      841 actggccaaa tccgggacaa ccacgaaaat gaatccggat ttctataaga agccggcctg
      901 attggttgtt gcccgccaga attggcaatc gttatctaaa agccctgcac ggttttactg
      961 tgcagggctt tttttggcca agcactgcag ttggagtgat tcgccggtag cctaagtgga
     1021 aatactttta gtcctacttg gttatttgct gggttcggtg cctgtgggct tcattctcgg
     1081 attgagatca ggcattgatg tcagagagac gggtagcggt aatgtcggcg cgacaaatgt
     1141 cgccagggta gtaggcaagc gtcaaggtat cttgaccttg gtcgcggaca cggcgaaagg
     1201 tttccttccc gtcattctag ctatacaact cggggcgagc cgtgcggcaa caacattggt
     1261 cggtgcggct gcgtttctcg ggcacctctt tccaatcttt ttgaagttca agggaggaaa
     1321 aggggtagct acggcttttg gtgttttcct ggctctagct ccgacggcca cgctggtgtt
     1381 ggttgcgcga tttggcgtca cgtttttaac cagcaggatc gtttcgctaa gctcgatttt
     1441 aagcgctgtg gcggcgccga tattactttg gttgttttct tacccgcgat tggtgatcgg
     1501 catggctgcc tttattgccc tggcaatcac ctggcggcat caaagtaaca tccggcggtt
     1561 gatgaacgga agcgaacctc gcttcggatc cgctaattct caatgatcac ctccacccgg
     1621 cgattttgcc ttcgaccctc ctcggaatca ttgctggcaa ttggtgcgcg ggtgcctaac
     1681 ccttgcgacg aaatgcgccc gctattaatg ccgttacgct ccagcgattg ggcgacggtg
     1741 cttgcccgtc gctcggacag ccgctggtta taagactctt gcgcggcgct ttcgcggctc
     1801 gcgtggcctt ccaccgttat cttgcgatta ggggcctctc gctgcaggat attagcaatc
     1861 tgatttaccc gatctcgccc atccggagtt aagtcagcgc tatcaaattg aaagttgacg
     1921 ttgggaaggt tgaccgttac gcctcggctg gtttctcgtg cctcaatgtt tcgctttttt
     1981 agttcatcaa tcagcgcctg gttcttagca atttcagctc gctgctgatc gatggctttt
     2041 tgctgctcgg cttgttttat gtcctgtccc tgcagttgat cgccgatgag agctcccgta
     2101 cccaagccta gcgccccgcc gaccgccgcg cccacgcccg gtcgtccgac tgcagcgccg
     2161 acaatcgcac cggtagccgc gccgccgaga gcaccgattc ctgcgccttt ctctcgggtg
     2221 cttaatgttc caccggaaca cccggctgcg accatcgaca atgcgattac cgctgctgta
     2281 aaaataggag aattgttcat cgtacctttc tcgatctgtc tttgactatc gatttaatta
     2341 gtattccccg gtttttttga gcccttccag atcgcgtctt tgccgctcca actctgcctg
     2401 gttcgcatcg atctggcgct gctgctccga ctgtttttgc tcctggcctt gtaattgatc
     2461 tccaaccaaa gctcctgatc ccagacccag cgcgccacca atcgccgcgc ctgcgccggg
     2521 tcgtcccaca gccgcgccga taataccgcc gaccgcagct ccgccaagcg cgccaatgcc
     2581 ggcccccttt tcgcgggtcg tcaaaccgcc gctcgagcaa cctgtagcag ataacaaacc
     2641 aaagccaaca ataatcgtga ctatccgtct catgtgacct ccagtgaatc ggcgcaaaga
     2701 ggcagtcctc gaagtaaaac aagtgtagca aaagccattt tgccgggcaa ctttttcaac
     2761 agaaacgcgc caccactgag gtatctattc agatatttag actcgtttgt ccgctgcttg
     2821 ttaacccgct taacgctgtt gacatcatag accttataag tctagagtct aagaaaggag
     2881 tgcaatggaa accttatctc aggagcgaat tctcggcgta ttaaagaagg ttcaggaccc
     2941 tgaacttcac cgcgatatcg tgagtcttgg aatggtaaag aaccttgtcc tgaaagacgg
     3001 caaggttagc ttcacagttg aattgacaac cccggcttgt cctttgcgcg agaccatcga
     3061 aacggattgt aagaaggcat tggcggaggt tcccgggatc aaagggcttg aaataagttt
     3121 tggcgcgcaa gtgcgcggta gcaaggcagg cgagggtcaa acagatctgc ttccgtctgt
     3181 gaaaaacgtc gtgctggttg ccgccggcaa gggcggtgtc ggtaaatcca cggtagcggc
     3241 taatctggca gtcgctctaa aaaagcatgg cgccgcaacc ggattgcttg acgcggatat
     3301 ctatgggcct tcggtgccga ttatcatggg ggtcaaaggt gagccggcga aagtcgaggt
     3361 caacggcgct ttcaagattg ctccgacaat agctcatggc gtgccggtga tgtcgattgg
     3421 cttttttctt gatcctgagc aggcggttat ttggcgcggg ccgatgcttg gcaaggcgct
     3481 gcaccagctc atggccgacg tgcattgggg cgaactggac tatctggtag tcgacatgcc
     3541 gccgggaacc ggcgatgtgc aaattacctt ttcccaacag ttgaaagtca gcggcgcgct
     3601 cttagtcgcc acgccgcaag atgttgctct cgccgacgtc attcgggcca agacgatgtt
     3661 cgacaaagtg atgattccga ttgtcggcgt ggttgagaac atgagttatt tcatttgcga
     3721 cggttgtaac aagaagcacg agattttctc ccgtggcggg gcgctcaggg cagcggagcg
     3781 gtttaatatt ccctatctcg gggaaatccc gatcacgccg gcgcttcgag aaggagggga
     3841 caaggggtta ccgatcctca ttcaagatcc taactccgag gtaagcaaag tttttctaga
     3901 gatcgcggcc aagttggccg ggcagctctc gattgcttcg gaacgcgcca agcaaactca
     3961 aggtctgaaa attgtttcaa gctagtttca atttcgtgat taattagccg gtatccggag
     4021 gagtagaatc aatggttaca aaagaacaag tctatgaagc actgcacgaa tgttatgatc
     4081 ctgaaatccc ggtcaatata gtcgatctcg gtttggttta cgatgtcgaa gtgaataacg
     4141 acaatgtcgc agtgaaaatg accttgacgg cgccggggtg cggcatgggg acgatgatcg
     4201 ccgccgatgc gaaagaaaaa attctcgctt tagagggtgt gaatgacgcc actgttgatc
     4261 tcgtgtggga tccgccatgg gatccgagca ggatcagtga cgaggcaaag cagagactcg
     4321 gaatcggctg aaactctgct cgatccgctg ctgcagcagc acaatccgcg gttcccacaa
     4381 ctccttgagc taatcgcagg ctctttcgtt taacgatgaa aagttttccc cgtttg
//
LOCUS       DASUTG010000859         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2209386, whole genome shotgun sequence.
ACCESSION   DASUTG010000859 DASUTG010000000
VERSION     DASUTG010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209386"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VF089_20030"
     CDS             complement(<1..1625)
                     /locus_tag="VF089_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX6176304.1"
                     /translation="MRADAHQKVNTGHLKRNAYLYIRQSTLRQVFENSESTFRQYDLR
                     QHALALGWAPEQIVVIDNDLGQSGASAVGREGFQRLVTEVSMGHAGIVLGLEVSRLAR
                     NSIDWHRLLEICALTETLILDEDGVYDPAHYNDRLLLGLKGTLSEAELHVIKARLQGG
                     ILNKARRGELESPLPVGFIYNPVRQPVLDPDKQVQESLQFFFDTFTRTGSAMATMKTF
                     HQRGLLFPRRLRKGPQKGDLVWAELTHSRALQVLHNPRYAGAFVYGRKRVRKKADGSE
                     ECLKRPRDQWILLPGMHPGYISWEQYEENQRRLQENAQAQGDDRRKSPPREGPALLQG
                     LVLCGVCGRRMTVRYHSRCTQPVPDYVCQKDGIEHGKPICQSINGEQIDKSISDLLIQ
                     TMTPMALDIALAVQQEVQTRLDEVDRLRRKQVERARYEANQAQRRYMHVDPANRLVAD
                     TLEAEWNAKLRALNEAQQEYERLRQTDHLAIGAAQRTRIAALASDFPRLWQDPKTPDR
                     ERKRMVRLLLEDVTLLKGEQVHVHIRFKGGVLR"
     gene            complement(1911..2390)
                     /locus_tag="VF089_20035"
     CDS             complement(1911..2390)
                     /locus_tag="VF089_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6176305.1"
                     /translation="MLKSQPPHDSKLRSLREQGALNPRPQAVIDDLFIASEFFDPRDL
                     VQVKYEMLRRVEKDGQSVTEAAAKFGFSRPSFYQAQSALAQRGLAGLIPLKRGPKQAR
                     KLSTAVMDFIRQVLQEDSSLSTRDLARRIRERFNVTVHPRTIERGLARDQKKRRKPE"
     gene            complement(2596..2871)
                     /locus_tag="VF089_20040"
     CDS             complement(2596..2871)
                     /locus_tag="VF089_20040"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
                     /protein_id="HEX6176306.1"
                     /translation="MAPHENGARRHFRVTHPFHPLFSREFELIEYHKTWGEDRVFFRE
                     AEGHLQSIVASCTDVGGVDPFVEIAAGRSFFRYEDLVRLADLLEGLR"
     gene            2874..>3285
                     /locus_tag="VF089_20045"
     CDS             2874..>3285
                     /locus_tag="VF089_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX6176307.1"
                     /translation="MTLSKGKVERAIGYIRQSFWPLRSFTDLADVNAQVRQWTRDTAN
                     ERKHRETGQAPRDRFQPEFLHAVPLLAPDYRDTAEALVQKDLRLCFDGNRYCVPPRYV
                     GAKLTVKADSGSVTIFDQSKEVVRYSRSWERGRTF"
BASE COUNT          653 a          992 c          906 g          734 t
ORIGIN      
        1 ctcctcagta ctccaccctt gaaccggata tgtacgtgga cttgctcgcc tttaagcaga
       61 gtgacatctt ctaatagcag acgaaccatt cgtttccttt cgcggtcggg tgtcttgggg
      121 tcttgccaca agcgcggaaa atcgctggcc agcgccgcga tacgtgttcg ttgcgccgca
      181 ccgatagcga gatgatcagt ttgacgcagc cgctcgtact cttgctgcgc ctcgttcaac
      241 gcgcgcaact tggcgttcca ttccgcttcc agagtatcgg caacgagccg gttggccggg
      301 tccacgtgca tgtaccggcg ttgagcctgg ttggcttcgt aacgcgctcg ctccacctgt
      361 ttgcggcgta accgatctac ctcatccagg cgagtttgga cctcttgctg cacggccaac
      421 gcgatgtcga gcgccatggg cgtcatcgtt tgaatcaaca ggtcgctgat ggatttatcg
      481 atctgctccc cattgatgct ctggcagatc ggcttcccat gctcgatgcc gtccttctga
      541 caaacgtagt cgggcactgg ctgcgtgcag cggctgtggt atcgaaccgt catccgcctt
      601 ccgcaaacgc cacacagaac caacccttgc aacaaggctg gaccttcgcg tggcggactc
      661 ttgcggcgat catccccctg cgcctgggca ttctcttgca gccgacgttg gttctcttcg
      721 tactgctccc acgagatata gccgggatgc atgccgggca ataaaatcca ttgatcccgc
      781 gggcgcttca ggcattcttc gctgccgtcg gctttcttgc gcacgcgctt ccttccatac
      841 acgaaggccc ccgcgtagcg cggattgtgc aagacctgca aagcgcgact atgcgtcaac
      901 tctgcccaaa ccaagtcgcc tttctgcggc cctttcctca atcgccgggg aaacaggagt
      961 ccgcgctgat gaaacgtctt catcgttgcc atcgccgatc ccgtacgagt aaacgtatca
     1021 aagaagaatt gcaggctttc ttggacctgt ttgtctggat cgagaaccgg ctggcgcacc
     1081 ggattataga tgaagccgac cggcagcgga gattccaatt cgccccggcg agctttgttc
     1141 aaaataccgc cttgcaaccg ggctttaata acatgaagtt ctgcctccga taaagtcccc
     1201 ttcaatccca aaagaagtct gtcgttatag tgagcgggat cgtagacgcc atcttcatcg
     1261 agaatcagcg tctcggtgag cgcgcagatt tctagcaggc ggtgccagtc gatcgagttc
     1321 cgcgccagcc gcgagacctc cagccccagc acgatgccgg cgtggcccat actcacctcg
     1381 gtcaccagac gttggaaccc ctcgcgccct acggcggacg cgccggactg accgagatcg
     1441 ttgtcgatca ccacgatctg ctccggcgcc cagcctaacg caagcgcgtg ttgccgcaga
     1501 tcatattggc ggaaagtgct ctcgctgttt tcgaacacct gccggagcgt ggactgccgg
     1561 atatataaat aggcgttgcg cttgagatga ccagtattca ctttttggtg agcgtctgct
     1621 ctcatatggt ggcctccagt tgttgtccca gaatcagtcc ggccagaagg atggccagtt
     1681 gcgcccggat gtcctgcgga caactcgaga tggccatcgc tggcggggca accgccgccg
     1741 cagtgttgga catacaagga gaccaagctc gcatccaggc aatcattcct tttcgcaaaa
     1801 acagcgccaa cccgggccct gcggagcgac tggccactgg gcacaggaca tcttttcgaa
     1861 gctgctcgta atgagcgacc agttcgtcgt ttggctggcc actgctgagt tcattctggc
     1921 ttccgtcgtt ttttttgatc ccgcgccagc ccgcgctcga tcgttcgcgg atgcaccgtc
     1981 acgttgaagc gttcccggat tcgtctggcc aggtctcgag tgcttaaaga ggaatcttcc
     2041 tgcagcacct ggcggatgaa gtccatcaca gctgtactga gcttgcgcgc ttgtttgggc
     2101 ccgcgcttca gtggaatgag acccgccaag ccacgctgtg caagggcgga ctgggcctga
     2161 tagaacgacg gccgcgagaa accaaacttt gcggcggctt cggtgacaga ttgcccatct
     2221 ttctcgacgc ggcgcaacat ctcgtatttg acctgaacca agtcgcgggg atcgaagaac
     2281 tcgctggcga tgaacaggtc gtcgattacg gcttgcgggc gcgggttcag ggcaccctgc
     2341 tcgcgcaggc tgcggagttt ggagtcgtga ggaggttgcg atttgagcat caccattctc
     2401 atcaaggaca gactgtaaat ataattatgt cacctaaggt ctggctgtca agctttttct
     2461 ctcgctaagt gacgcgtcta tttggcattt ataatgtata acagcgcata tctcttcgag
     2521 acacggcctt gcatacggcg cagttctctt tacctaagcg gcattattat ctttacacgg
     2581 actggcattc tgccctcagc ggagtccctc cagcagatcc gccagtctga ccaagtcctc
     2641 gtagcgaaag aaggatcgcc cggcggcgat ttcaacgaac ggatcaacgc ctccgacatc
     2701 cgtacagctc gccacaatcg attgcaggtg gccttcggct tcacggaaga acacgcgatc
     2761 ctcaccccag gtcttgtgat actcgatcaa ctcgaactcg cggctgaaca agggatggaa
     2821 gggatgggtt acccgaaaat gtctgcgcgc accgttctca tgcggtgcaa ttgatgactc
     2881 tttccaaagg caaagtggag cgggccatcg gctacattcg ccagagtttc tggcctcttc
     2941 ggtccttcac cgacctcgcc gacgtgaatg cacaggtccg ccagtggaca cgcgacactg
     3001 ccaacgagcg caaacaccgc gaaaccgggc aggcgcccag ggatcgcttt cagcccgagt
     3061 tcctgcatgc cgtgccgttg ctggcgcccg attatcgcga taccgccgag gcactggttc
     3121 agaaggacct ccggttgtgc tttgacggca atcgctactg cgtaccgccg cgatatgtcg
     3181 gcgcgaagct caccgtcaag gcagattccg ggtcggtgac catcttcgac cagtcgaagg
     3241 aggtcgtgcg ttactccaga tcgtgggagc gcggtcgcac ctttg
//
LOCUS       DASUTG010000860        10660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_873562, whole genome shotgun sequence.
ACCESSION   DASUTG010000860 DASUTG010000000
VERSION     DASUTG010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873562"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF089_20050"
     CDS             <1..659
                     /locus_tag="VF089_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit alpha"
                     /protein_id="HEX6176308.1"
                     /translation="HMLMDSNFGDPYRLDKRAPCVGEHQLHMNPQAARDLNINDGDYV
                     YIDANPADRPYLGAKPGDPFYRVSRCMLRVTYNRAYPYNIVMMKHAPFIATEKSVKAH
                     ETRPDGRALSENTGYQANLRYGSQQSVTRNWHMPMHQTDTLFHKTKAFMGFIFGGEAD
                     NHAVNTVPKETLVRVTKAEDGGMGGKGIWKPATTGFSPDNENELMKRYVAGDLTKVKT
                     "
     gene            711..1157
                     /locus_tag="VF089_20055"
     CDS             711..1157
                     /locus_tag="VF089_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176309.1"
                     /translation="MPFKDPDPTDPNLLVGVMLPADAEATRDMAYVFAEEFARFGYTR
                     EQLLWLFKNPFYGGAHGAYRSLGEQETLSIIDECLNVWGSIKVRVQDIPNVQNVQAPT
                     SVLPRVAGEEGSGGSNECNLPNGLNGTQPLNDLNVLNRRREERGDE"
     gene            1150..2415
                     /locus_tag="VF089_20060"
     CDS             1150..2415
                     /locus_tag="VF089_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6176310.1"
                     /translation="MSNVYNWQIGREMNYPYTQAFPKRQFAFVFNINRCIACQSCTMA
                     CKSTWTFSKGQEHMWWANVETKPYGGYPQFWDAKILDLLEKANPGNQQWSGQPSADKK
                     KPYGQFDGKTIFEAQNMLTPDSARVLGYLPTDEEWNLPNIFEDNPVGKKGVRNELDKE
                     GAALPEHKTWFFYLARICNHCSYPACLAACPRKAIYKRPEDGIVLIDQKECRGYRKCV
                     EACPYKKTMYRGNTRVSEKCVACYPRIEGKDPESGGQPMETRCMAACIGQIRMQGLVK
                     MNNDGTWAEDRYNPLYYMVHVAKVALPLYPQFGTEPNGYYIPPRWVPRDYLKQMFGPG
                     VDEAIERYSYPDRELLAVLQLFRRSNLIIDRYEIKEGEKIYEAELRGRKITMYNDTVI
                     AYGKNGKEIFRTQVEEPVHVRPNKHANSI"
     gene            2489..3280
                     /locus_tag="VF089_20065"
     CDS             2489..3280
                     /locus_tag="VF089_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX6176311.1"
                     /translation="MSVNPEPSELELAFCRATLYSALALGFRPPTEEAIARLTTAQNA
                     AALGEAAAIVDSDGKLGLVSLVQAVAHAGAAGATVLMASYRALFGHAVRGPIPPYETE
                     YGNEALFQQPQEMADLMGFYRAFGLTVRVDGHERCDHISCECEFLSFLALKEAYALEH
                     RDPDMLESSRKATRLFLRDHLGRFLPAFAGKLRREDRAGFYSMLVQLCLAFVELESNR
                     LDVPRGAASLGLRPIDDDRIPMACGSGDECAALPGACAPEGADSV"
     gene            3277..4287
                     /locus_tag="VF089_20070"
     CDS             3277..4287
                     /locus_tag="VF089_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176312.1"
                     /translation="MSQTILIRYDPRLIEEAVFLALRNHPDGRQYQVERECVYEIADA
                     RERDRVFGELNKEWLVRLGLNKAVEQSLAEQPEISSRSSSCFVGRVAQTKEEGAELFV
                     APEEQLEVTDCRIVSILLRPESMLQPETLLTFLRHELFHVADMLDPAFAYQPALPKAE
                     GGPTYDTLITNRYRALWDVTINGRMMRRGWLPATAREQQLGDFLNAFPMFEENHQEFF
                     GPFFDAEQPKHAELVAFAFDPRAASGRQIHEPAPGNHCPLCRFPTHAFEPEPDNLGAA
                     VLEVIAEDFPNWTPSRGLCTQCADLYRGRQTSMDALRLLPGWTPRQPEHERGTPPAVP
                     RG"
     gene            4296..5852
                     /locus_tag="VF089_20075"
     CDS             4296..5852
                     /locus_tag="VF089_20075"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX6176313.1"
                     /translation="MIPVHIAGVGMTQFGKSSHTLVEIMRDAAVTALAVSKIQDVDAI
                     YIAVMNPEEYTGDANIASHVAEALGFRGLPAVRVETASSAGAAAVHAAYHGVASGYYR
                     HVLVVGGEKMTHLTTSASTRILAEVIDAQERNCGATMPALAAMITARYRKKYRLSEST
                     LAQVLCAVAMKNHANGVHNSCAQFRQVITQEKYFASKFVATPLRLYDCAPMTDGAAAI
                     ILTGERTDVAVCGIGQGTGPVSLRERETFTCFPATQIAARRAYQMAGISPREIDCAEV
                     HDAFTPFEIIGTEDLGFFPAGKGMQAAASGQTAADGNLPINASGGLKSRGHPVGASGV
                     AQIVEIVHMLREESTAKLRRAPHRGLAQSIGGLASNNFVTILERSKEVAQLRRCSIAP
                     ASRHLEPPRAERKPCAGPIESEGVIDTFTIVYTTPDGFLPPLALAMIRDRKGVRIMAQ
                     AEDISHLQIGREVYLHPVAGIYYFTVKKQLQKVRAAMQKILHRAGRNSKRTKIRRLKA
                     NTLKIPLRRV"
     gene            6148..6840
                     /locus_tag="VF089_20080"
     CDS             6148..6840
                     /locus_tag="VF089_20080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6176314.1"
                     /translation="MAKKITARSEKQFRSITKLIAEELRDAIVAGKIAPGERLTEDQL
                     TGKLKVGQVPLRDALRRLETEGYLTTVSHGQFMVSKLALEDAEDYYSIAGALEGLAAR
                     LAVERGSAEEISRLCDLHQMLKLAYREKNPEQYFEANRQFHRFIAQMSRNDRLYQLIS
                     EMRRGLRKARLLALQLPQRTDYSMREHDQILDAFLKRNPDLAESTVLRHLANQLTAIK
                     TILQSAEGSSHA"
     gene            6922..7365
                     /locus_tag="VF089_20085"
     CDS             6922..7365
                     /locus_tag="VF089_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6176315.1"
                     /translation="MSLDRICSKTVVTISPDATVLEAAKLMHSKHIGCLVVTDNSRPT
                     GILTDRDIVLKLVAGEKKPEETTVKNIMTTNPTMVNVNYDVLDAVRLMRDRGVRRLPV
                     IDEHRHLLGIITLDDVLTAFGAELGDLAGAVQKEFGLEAAASVGT"
     gene            8185..8532
                     /locus_tag="VF089_20090"
     CDS             8185..8532
                     /locus_tag="VF089_20090"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX6176316.1"
                     /translation="MAVKILIQRKVRPGRENEFGEALRKLRSKAIHAQGYISGETLHS
                     VEEPSLHLVISTWKSLEDWNRWANSPERKAFGENTDAMLQEPAKVTPYQYESNLPNVD
                     AILSSLEFSIQDE"
     gene            8619..9599
                     /locus_tag="VF089_20095"
     CDS             8619..9599
                     /locus_tag="VF089_20095"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176317.1"
                     /translation="MKKAAPLTILAVVLAAAVMAEAQQAGKIPRIGVLMPGSRSTAKL
                     RLKAFHEGLRELGYVEGKNIVLEYRYAEGRPETLPKRVDELVQLNVDVIVTATSNATQ
                     AAKKTTTTIPVVFATANDPVGDGHVASLARPGGNITGFSNLAPELNGKKLELLKETVS
                     KLTRVAFLLRWGTSIGEQRFNETEIAAKDLGLRRLPLSAKGHEDLESAFSAVKSAGAE
                     ALIVSPSTFSIEHRRRLIELTAKYRLPAIYPSPLFVEAGGLMSYGPDLINSWRRATIY
                     VDKILKGTKPTELPVEQPKKFEFAVNLTTAKQIGLTIPPDVLARADKVIK"
     gene            complement(10032..>10660)
                     /locus_tag="VF089_20100"
     CDS             complement(10032..>10660)
                     /locus_tag="VF089_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6176318.1"
                     /translation="GISKDDLSCLFEMFFQVDRSFSQAHGGLGIGLTLVKRLVEMHGG
                     TVEAKSAGINQGSEFLVSLPMLVSSSAVLMNKDAVEQKYETRRRILVVDDYPDAAESL
                     ARRLKHLGNEVETALDGFEGIKQAEKFRPDIVLLDLGMPKLNGYKAAQQIREQPWGKD
                     MVLIAITGWGQEEDRQRTREAGFDAHLVKPMDYTKLGELLEQFAGASS"
BASE COUNT         2608 a         2873 c         2904 g         2275 t
ORIGIN      
        1 ggcatatgct gatggactcg aacttcggcg atccttaccg gctggacaag cgcgcgccgt
       61 gcgtcggcga acatcaactc cacatgaacc cgcaggcggc tcgcgatctc aacattaacg
      121 acggcgacta cgtctatatc gacgccaacc cggctgaccg gccttacctc ggagccaaac
      181 caggcgatcc tttctaccgg gtatcgcgtt gcatgctgcg cgtcacctac aaccgcgctt
      241 acccgtacaa catcgtcatg atgaaacatg cgcccttcat cgccacggaa aaaagcgtaa
      301 aggcacatga gaccagaccg gatgggcggg ccctgtcgga gaacaccggc taccaggcca
      361 atcttcggta tggctcgcag caatcggtca cacgcaattg gcacatgccg atgcatcaga
      421 ccgacacgtt gttccataag accaaggctt tcatggggtt catcttcggc ggcgaagcgg
      481 ataaccacgc ggtcaacacc gtacccaaag aaactctggt gcgagtcact aaagcggagg
      541 acggcggcat gggcggtaaa gggatttgga agccggccac tactggtttt tcgccggaca
      601 acgaaaacga gctgatgaag cgctatgtgg ccggagattt gacgaaggtg aaaacatgag
      661 attagaccgt tggaaccgtt ggaaatcgag ctacgatagg agcgagaaaa atgcccttta
      721 aagatcccga ccctaccgat ccgaatctgc ttgtcggcgt gatgctgcct gctgatgccg
      781 aagcgacgcg cgacatggcg tatgtcttcg ccgaagagtt cgcgcgattc ggctacacgc
      841 gagagcaatt gctttggcta ttcaagaatc cgttttatgg cggagctcat ggcgcctacc
      901 ggtcgcttgg agagcaagaa acactgtcaa tcatcgacga gtgtttgaac gtttggggaa
      961 gtatcaaagt gagagttcaa gacattccaa atgttcaaaa cgttcaagcc cccacctctg
     1021 tcctcccccg cgttgcgggg gaggagggaa gcggaggttc caacgaatgt aacctcccga
     1081 acggcttgaa cggaacgcag ccattgaacg acttgaacgt tttgaaccgg cgacgagagg
     1141 agcgaggcga tgagtaacgt ttacaactgg cagatcggcc gggagatgaa ttatccctac
     1201 actcaggctt ttccgaagcg gcagttcgcc tttgtgttca acatcaaccg ctgcatcgcc
     1261 tgccagagct gcaccatggc ttgcaagtca acctggacgt tcagcaaggg ccaggagcac
     1321 atgtggtggg cgaatgtcga aaccaaaccg tacggcggtt acccgcaatt ttgggatgca
     1381 aaaattctcg acctattaga gaaggccaat cccggcaatc aacagtggtc cggccaacca
     1441 tcggccgata agaaaaaacc ctatgggcag ttcgatggca agactatctt cgaagcgcaa
     1501 aacatgctga ctccggacag cgcgcgggtg ctgggctatc ttccgaccga cgaggaatgg
     1561 aatttgccga atattttcga agacaaccct gtcggcaaaa aaggcgtgcg caacgagctc
     1621 gacaaagaag gagccgcgct ccccgagcat aagacctggt tcttctatct cgcgcgtatc
     1681 tgcaatcact gtagttatcc tgcctgcctg gcggcctgcc cgcgtaaggc gatctataaa
     1741 cgccccgaag acggcatcgt cctgatcgat cagaaagaat gtcgtggcta ccgcaagtgc
     1801 gtcgaagcct gcccgtacaa gaagaccatg tatcgcggca acacccgcgt ctccgaaaaa
     1861 tgtgtcgctt gctatccgcg gatcgaaggc aaggatccag agagcggcgg gcagccgatg
     1921 gagacccgct gcatggccgc ttgcatcggc cagatccgca tgcagggttt agtcaaaatg
     1981 aacaacgacg gcacctgggc ggaagaccgt tacaacccgc tctattacat ggttcatgtc
     2041 gccaaagtcg ccttgccgct ttacccgcag ttcggcaccg agcccaacgg gtattacatc
     2101 ccgccgcgtt gggtgccgcg agattatttg aaacagatgt tcgggccagg ggtcgatgaa
     2161 gccatcgaac gctacagcta tcccgacagg gagttgctcg ccgtgttaca gctctttcgc
     2221 cggtcgaatc taatcatcga ccgctacgaa atcaaagaag gagagaaaat ttacgaggcc
     2281 gaactgcgcg ggcgcaaaat caccatgtac aacgataccg tgatcgctta cggcaagaac
     2341 ggcaaagaga tttttcgcac tcaggtcgag gaacccgtac acgtgcggcc caacaaacat
     2401 gcaaattcaa tatgagatcg gacccttcaa tctctgaagc gctcgaacaa tttgacgact
     2461 tgaagacgga gtggagaaaa tgaacccaat gagcgttaac ccagaaccaa gcgagcttga
     2521 gctcgccttt tgccgcgcaa ctttatatag cgcgctagcg ttgggctttc ggccgccgac
     2581 cgaagaggct atcgctcggc ttacaacggc gcaaaacgcg gcggctctcg gagaagcggc
     2641 ggccatagtg gatagcgatg gcaaactggg gctcgtctca ctggtccagg ctgtcgctca
     2701 tgcaggtgct gccggcgcaa ccgtcctcat ggcttcatat cgagccctgt tcggtcatgc
     2761 tgtgcgcggt ccaattcccc cctacgagac cgagtacggc aacgaagcgc tttttcagca
     2821 accgcaggag atggccgact tgatgggttt ctatcgtgct ttcggcctca cggtcagggt
     2881 tgatgggcat gaacgctgcg atcacattag ctgtgaatgc gagtttctgt cttttctggc
     2941 gctcaaagaa gcctatgcct tagagcaccg cgatccggat atgttggaat cgtcgcgcaa
     3001 ggccacgcgc ctgttcctac gcgatcatct tggccgattc ctgccggcat ttgccggcaa
     3061 gctaaggcga gaagaccgag ccggctttta cagcatgctg gtccagcttt gccttgcatt
     3121 cgtcgaactg gagagcaacc gtttggacgt tccacggggc gccgcaagcc tgggtttacg
     3181 tcccatcgat gatgaccgga tacccatggc ttgtggcagc ggcgatgaat gcgcggcgct
     3241 gcccggcgcc tgcgcgccag agggagccga ttccgtatga gccagacaat tttgattcgc
     3301 tacgatccgc gcttgatcga ggaagcggtg tttctagctt tgcgcaacca tccggatgga
     3361 aggcaatatc aagtagagcg cgaatgcgtc tatgagattg ccgatgcgcg ggaacgagac
     3421 cgggtattcg gcgagttgaa caaagaatgg ctcgttcgac ttggcctgaa taaagcggtc
     3481 gaacaatctt tagccgagca gcccgagatt tcttcgaggt cgagcagttg tttcgtgggt
     3541 cgggttgcgc aaaccaaaga agaaggcgcc gagctttttg tcgccccgga ggagcaactc
     3601 gaagtaactg actgccggat cgtgagcatt ctgctccggc ctgaatcgat gctacagccg
     3661 gagacattac tgacttttct ccgccacgaa ctgtttcatg tcgccgacat gctcgacccg
     3721 gcatttgctt accagccggc attgccaaag gcggagggcg gtcccaccta cgacacgttg
     3781 attaccaatc gctatcgggc gctatgggac gtgacgatca acggccggat gatgcgccgc
     3841 ggctggctcc cggcaaccgc gcgcgagcag cagctgggtg attttctcaa cgcttttccg
     3901 atgttcgaag aaaatcacca agagtttttt ggaccgttct ttgacgccga gcagcccaaa
     3961 cacgccgaac tcgtcgcctt tgcctttgat ccgagagctg ccagcgggcg tcaaattcac
     4021 gaacccgctc ccggcaatca ttgtccctta tgtagattcc cgactcacgc cttcgaacca
     4081 gaacccgata atctcggggc cgccgtgcta gaggtgatcg cagaagattt tcccaactgg
     4141 acaccttcgc gcgggctgtg cacgcagtgc gctgatcttt accgcggccg acaaacctcg
     4201 atggatgcct tgagacttct gccgggatgg acaccacgcc aacccgagca tgagcggggc
     4261 actccaccgg cagtgccgag aggctgattg gacagatgat tcccgtccac atcgccggcg
     4321 tcggcatgac ccagtttggc aagagctcgc acacgctggt cgagatcatg cgcgatgccg
     4381 cagtgactgc gctggcggta tcaaagatcc aagatgtcga tgccatttac attgcggtga
     4441 tgaatcccga agaatacacc ggcgacgcta atatcgcttc acatgtcgcc gaagcgctcg
     4501 gattcagagg gctcccggcg gtgagagtgg aaacggcatc ttcggctggc gcggcagcgg
     4561 ttcatgccgc gtatcacggt gtagcgtcgg gatattaccg ccacgtatta gtggtcggcg
     4621 gtgaaaagat gacccatctg accaccagcg cgagcacgag aattctcgca gaagtcatcg
     4681 acgcccaaga gcgtaattgc ggcgccacca tgccggcgct ggccgccatg ataaccgcca
     4741 ggtaccgcaa gaaataccgt ctttcagaat ccactctggc acaggttctc tgtgcggttg
     4801 cgatgaagaa tcacgccaac ggagttcata attcctgcgc tcagtttcgc caagtcatca
     4861 ctcaagaaaa atattttgcc agcaaattcg tggcaacccc cttacggctg tacgactgcg
     4921 cgcctatgac cgacggcgcc gccgcgatta ttcttaccgg cgagcgcacc gatgttgcgg
     4981 tctgcggaat cggtcagggt accggcccgg tgagtttgcg cgagcgagaa acctttacct
     5041 gtttcccggc gacgcaaatc gccgccagga gagcttatca aatggccggc atctcgcccc
     5101 gggaaatcga ctgcgcagag gttcacgacg ccttcacacc cttcgaaatc atcggcacgg
     5161 aagaccttgg cttctttccg gcaggtaaag gaatgcaagc cgctgcatcc ggacagacag
     5221 cggcagatgg gaatctgccg atcaacgcgt ccggtggcct caaatcacgc ggtcatcccg
     5281 tgggcgccag cggtgtggct cagatcgtcg agatcgtgca tatgctgcga gaagagtcta
     5341 cggcgaagct caggcgcgca ccgcatcgag gcctggcgca aagcatcggc gggctcgctt
     5401 ccaacaattt cgtcaccatc ctagaacgtt cgaaagaagt cgcgcagctc cgccgttgtt
     5461 cgatagcgcc ggcaagccgc caccttgagc cgcctcgggc ggaaaggaaa ccctgcgctg
     5521 ggcccataga aagcgagggc gtgattgata cctttacgat tgtttacaca actccggacg
     5581 gtttcttacc gccgttggct ctggccatga tccgcgatcg taaaggagtg cgaatcatgg
     5641 cccaagcgga agacatcagc cacttgcaga ttggccgcga ggtttacctg catccagtcg
     5701 cagggatcta ctatttcacc gtgaagaaac aactacaaaa agtccgtgcc gccatgcaga
     5761 aaatattgca cagggctggt cggaattcga aacggacgaa aatacgccga ctgaaagcaa
     5821 acactttaaa gataccattg cgccgagtgt gatgagggaa gaagcagcgt gctcgtagag
     5881 agatgaaagg aatccgtcat gatacggcaa ggttggcttc gggacgaacc atcggagagg
     5941 gagtatcatc ggttacaaag agagatggaa gcgatacaaa agcggctgag ggagttatct
     6001 acttgtgagt tgcaagtaga acgagagctt aactgcggtt accatgaacg acccgcagcg
     6061 gcttagacga ttaagagttg ctgtttcagc cgcgcaacga tgtgtgcttg atagacttgc
     6121 gcgagcggct gatgagagag aatcagcatg gccaaaaaaa tcacagcccg atccgagaaa
     6181 caatttcgtt caataaccaa actgattgcc gaagagcttc gcgacgcgat cgtcgccggc
     6241 aagattgcgc ccggcgagcg tctgacggaa gatcaactca ccggaaagct caaagtagga
     6301 caggttccat tgcgcgacgc cctgcgacgc cttgaaacag agggctacct aacgaccgtt
     6361 tcccacggcc agttcatggt gagcaaactg gctctcgaag atgcggagga ttattattcg
     6421 atcgctggcg cgcttgaagg gttagccgca cgattagcag tcgagagagg cagcgctgaa
     6481 gagatttctc gattatgcga cttgcaccag atgctcaagc tcgcctatcg cgaaaaaaat
     6541 ccagagcaat actttgaagc caaccgacag tttcatcgct tcatcgccca gatgagcaga
     6601 aacgaccggc tttatcagct gattagcgaa atgcgtcgcg gcctacgcaa ggctcggtta
     6661 cttgcactgc aactgccgca acggacggat tactcgatgc gcgaacacga tcagattctc
     6721 gatgcctttc tcaagcgcaa tccggatctc gcggagagca ccgttctaag acacctggcc
     6781 aatcagctga cagcgatcaa aacaatatta caatcggctg agggctcaag ccatgcttaa
     6841 cctcgaggaa tttttcaagg aatcgactgc cgtgaagttt cctctaacgt ttaaatctat
     6901 tttctttttt ggaggtgtct catgagcctg gacagaatat gtagtaagac tgtggtgacg
     6961 atttctccgg atgcgaccgt gcttgaggca gcgaagctga tgcattccaa gcacattggc
     7021 tgcctggtag taaccgataa ctcccgccca acaggaatcc tcacggacag ggatatcgta
     7081 ctcaagcttg ttgccggtga aaaaaagccg gaggagacta cggtaaaaaa tataatgacc
     7141 accaacccca cgatggtcaa tgtcaattac gacgtgctcg atgccgtgcg gcttatgcgt
     7201 gatagaggcg ttcgacgtct cccggtcatc gatgaacatc gacaccttct cggaatcatc
     7261 accctggatg atgtgctgac agctttcggt gccgagttag gagatctggc aggggccgtt
     7321 cagaaagagt tcggcttgga agccgcggca tctgtgggga cctagttagc attaagtgat
     7381 cagtttaaag ccaacggcta atcagtacct tatgcaccgg agcccgtgaa gcgttccata
     7441 ctgacgacga ttggtcgctc gcaggcccgg tgatgccgat cgttgagcgg cggaggaaac
     7501 agcaggttca gcgtgacttc gcaatggaag gttgccgtga cggcgtttca ggaatctgaa
     7561 gtcgttctac agtactggtt ttccggttgt gaaaaccggg gatcaaatga ccatgctgcg
     7621 tcagactgaa tgatggttcc gaggagcgcg gatagcgata tacgcgacag tttcgctgag
     7681 ctactggagt tctcgatcgg atcgtattga cgcgtcgttc agttcaattc tgagctgaaa
     7741 atacaaccgc ttgacgcact ttctgcgtgt gatattgatc gcggcgtaac aagcctcccc
     7801 catcattgag caggcttctg gcatgatctg aggccaaagg aaggaacgca agccgttcct
     7861 gcgagggggg atatgaaaca gtgagtttag agccagccgc tccggcgatt tatcgctaga
     7921 gtatgctggc ttttttgttt cccaatgacc ggctacagac tcttaaagga gacctatatg
     7981 actacgatca cgggcaaccg gcagttcatc gactccgtac ttctacgacg agtacgccta
     8041 accgtgtggc tgcagaacgc cttgcttcct cgacagttgt gcgcacatac aaaatattca
     8101 gttgaacggg cttgaggcac cgcgtgagat tggactgact ttctctacag cgcaaaagta
     8161 tccacctttg attgaggaat tatcatggct gttaaaattc ttattcaaag aaaagttaga
     8221 ccgggcagag aaaacgagtt tggcgaggcc ctgaggaaac ttcggtccaa ggcaattcat
     8281 gctcaaggct atatttccgg agagaccttg cactccgtcg aagagccttc tctacatctg
     8341 gtgattagta cctggaagag tcttgaagac tggaatcgtt gggcaaactc gccggagcgg
     8401 aaagcattcg gagaaaatac agacgccatg ctccaagagc cagcgaaagt tactccttat
     8461 cagtatgaat cgaacttacc taatgtggac gcgatactca gctcacttga gtttagcatt
     8521 caggacgaat agctagtcgt accgcgcaca attaaaaaga ctttgtctca tcgagcaacc
     8581 gacctaagcg ataaataacg ctcgaaacga ggtgggtcat gaagaaagct gcaccgctaa
     8641 caatccttgc tgtagtgctt gctgctgcgg tgatggctga ggcgcagcag gcaggaaaaa
     8701 tcccacgaat cggggttctg atgcctggtt cgcgttctac cgcgaagctg cgccttaagg
     8761 cttttcatga agggcttcgt gagcttggct atgttgaagg aaagaacatc gtccttgagt
     8821 accgctatgc ggaaggtagg ccggaaactc tccccaagcg cgtcgacgag cttgtccaac
     8881 tcaacgtcga cgtgattgtc acagcgactt ccaatgccac tcaggcggcg aagaaaacga
     8941 ccacgacgat tcctgtagtc tttgccaccg ccaatgatcc agttggcgac ggacatgtgg
     9001 cgagtctggc gagacccggt ggaaacatca ctgggttttc aaatctcgcc ccagagttaa
     9061 atgggaaaaa gctcgaactt ctgaaggaga cggtttccaa acttacacga gtagcttttc
     9121 tcttgagatg gggcacatca attggtgagc aacgtttcaa cgagacagag atcgcggcaa
     9181 aagatctggg gcttcgccga ttacccctta gtgcgaaagg tcacgaggac ctcgaaagcg
     9241 ctttcagcgc ggtaaaaagc gccggcgctg aagctctaat tgtttccccg agcacttttt
     9301 caatcgagca ccgtcgccgg ctcattgagc ttacggcgaa atatcgctta ccggcgattt
     9361 acccatcacc gctatttgtc gaggctggag gcctgatgag ctatgggcca gatttaatca
     9421 acagctggcg ccgcgccaca atctacgtag ataagatttt aaagggtaca aaacccacgg
     9481 agctaccggt agagcagccg aagaagttcg agtttgcggt aaatctaacg accgccaagc
     9541 agatcggcct gacgattccg ccggatgttt tggcgcgggc ggataaggtg atcaagtgag
     9601 cgaaaaaagt aagcaacgct tgcaggatct ccttcagtac aatccaccat cggatcgaac
     9661 tgaggcgtcg ttcaattcaa tcccatgcga tttttgtgtc tttgcgccgg cgatagtttc
     9721 cgattgatct ttgtcagcca agccccgagc tcgcaaactt tattttcttc gttgaagcca
     9781 taagcactga agtcagctcg ccgtcgggaa agcacatgga agtgaagcgt ctttcaacaa
     9841 ttgctggcgc cgcgttctgc aacgtgcttt ctcattcatc aatggacaaa gcgtggcaaa
     9901 gtcattcgac aacgtaagcg acttcagcga catacacccg cgcagaggat taaataccgt
     9961 cggctggctg ctattcgatt ctacgttctg ctgctgatga tggaatctaa acggagtgtc
    10021 ttaggaatgt cctagctgct cgcacctgca aattgttcga gcagctcccc cagcttggta
    10081 tagtccattg gtttgaccaa gtgggcgtcg aatccagcct cgcgcgtacg ctgacgatcc
    10141 tcttcttgcc cccaaccggt aattgcgata agcaccatat ccttccccca aggctgttcc
    10201 cgtatctgct gggccgcttt atagccattg agtttcggca ttccgagatc gagcagcacg
    10261 atgtcgggac gaaatttttc cgcctgcttg attccttcga agccatcaag ggccgtttcg
    10321 acctcgttcc ctaagtgctt taggcgtcga gccaggcttt ctgccgcgtc tggataatca
    10381 tctaccacaa ggattcgtct gcgcgtttcg tatttctgct cgacagcatc tttgttcatt
    10441 aataccgccg acgaactgac cagcatcggc agagaaacca gaaattcgct tccttgatta
    10501 atacctgcac ttttcgcctc cacagttcca ccgtgcattt ctaccaatcg ctttacaaga
    10561 gttaggccaa tgccaagacc gccatgcgcc tgggagaagg aacgatcgac ttgaaagaac
    10621 atttcaaata agcaagacaa atcgtcctta gaaataccga
//
LOCUS       DASUTG010000861        10479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1023956, whole genome shotgun sequence.
ACCESSION   DASUTG010000861 DASUTG010000000
VERSION     DASUTG010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023956"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1606)
                     /locus_tag="VF089_20105"
     CDS             complement(77..1606)
                     /locus_tag="VF089_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016724698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX6176319.1"
                     /translation="MIPPCSFRHRGRILCCNVFVWLTFLFNCPLAVANGVFGFDQVAE
                     TARKLAAEPFKPPPPVPEFLKQISYDDYRDIRFDTAQSLWKDSGNFQVQFIHPGLFYT
                     HSVAINTIDSQGVRKVAFSPKQFSYGKNKFADKIPPELGFAGFRIAYPLYKKNEYNHV
                     IVFAGASYFRAVARKQVFGLSARGLAIDTALPTGEEFPTFREFWLERPSAQARTVKIL
                     ALLDSQSLTGAYEFIVRPGDRTLVDVKARLFERKRVKELGIAPLTSMFLYGEEKPRPN
                     TDWRPEVHDSDGLMMAAGSGEWIWRPLINPARLQVSYFDFDNLRGFGLLQRDRSYSNY
                     EDLETRHEMRPNAWVTPSGTWPKGQIKLVEIPSQKETNDNIVAYWIPRSMPAVGQPLD
                     LAYRINFQTEEPLAATSGRVTATRVGVGDKDDLKRIILDFEGGKLKSLPDTAPVKAVV
                     TVAGDAQLVQQSTVKNPITGGWRAAFQVKPPKDKPLQLRAFLRNETDTLTETWSYLLE
                     P"
     gene            complement(1809..2237)
                     /locus_tag="VF089_20110"
     CDS             complement(1809..2237)
                     /locus_tag="VF089_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176320.1"
                     /translation="MKQKSRRKSIAAAPLLFLVSSVGCESIALLPRPTIDDRADLSRR
                     DLDRRELDRRPESRADIPRPQADEVTGTVQKVDQSRREIQLRTTEGRMTVIKYDPATV
                     VYNREREMPVDDLRYGDLILIKVVKNSRGEQYADLIRLAS"
     gene            complement(2536..2904)
                     /locus_tag="VF089_20115"
     CDS             complement(2536..2904)
                     /locus_tag="VF089_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176321.1"
                     /translation="MKKRNPYATSPAARAARGIAGSASPLEKRQKPAMITFMETIKID
                     DPRLDESFAFMFQLRKGDAVVLKNQNSSRGKVSDGVYVGEFPKHVAGDIHPRGITLYE
                     VKLADDSVQILEEKELQKIE"
     gene            3202..4311
                     /locus_tag="VF089_20120"
     CDS             3202..4311
                     /locus_tag="VF089_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6176322.1"
                     /translation="MRATSQTKLPLALKIAAAGVAAAGFSCALRRYRRIDFSGKTVLI
                     SGASRGLGLELARRFAAQRANVVLMARDQERLTEAARELEAYGIEVSTRQCDVTQPEE
                     ARRAIRSVIDEFRSIDVLVNNAGIIQVGPAENMNRDDYADALAVHFWAPLNLTAEVLP
                     YMKGQGSGRIVNITSIGGKVAVPHLAPYVASKFALVGFSESMRAELIKDGIYVTTVVP
                     GLMRTGSHINAFFKGQHQKEFALFSIANASPLFSTASERAARQIVEACRYGKAELIIT
                     PQARLLHLANSLFPNITAEVLGLISRSLPSTRPGEGNALKRGWQSHSFMAPSVLTRTA
                     DRVIRRNQEQPRSAPGTNRSNGFAKGSAPERDRSR"
     gene            complement(4593..6014)
                     /locus_tag="VF089_20125"
     CDS             complement(4593..6014)
                     /locus_tag="VF089_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6176323.1"
                     /translation="MAQSQRYDAIVIGSGPNGLAAAITMIDSGRSVAVYEAGDKIGGG
                     MRSAELTLPGFVHDVCSAVYPLAIGSPLFRSLPLARYGLEWVQPPSPLAHALDDGSAV
                     LLERSLHDTAAGLGGDGAAYRRVMQPLAESWHGLDSDLLGPLRWPRKPLALARFGFSA
                     IRPARSFSQSCFKGQRARALFAGLSAHSMLPLEAWGSAAFGLVLATTGHVLGWPIARR
                     GSQSLADALALYLQSKGGEIYTNVPVRTLDELPSSRVILCDVTPKQLLALAGNRLSPS
                     YRRRLRGYRYGMAAFKIDWALSAPVPWKAKACARAATVHLGGTLQEIAASERATWRGD
                     HTEIPFVLAVQPSLFDSSRAPETKHTLWAYCHVPNGSTFDMTERIEAQIERFAPGFRD
                     TVLARSVAAPAELERRNPNLIGGDINGGAPTLNQLFLRPTLKLYSTSARGLYLCSAST
                     PPGGGVHGMCGYFAARQALKDFF"
     gene            6081..6284
                     /locus_tag="VF089_20130"
     CDS             6081..6284
                     /locus_tag="VF089_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176324.1"
                     /translation="MRDMRRDPVGVLWHVGSFDMIFARRTLLFSGMHNVSTALRAMTR
                     VITGTMSLELVGDIVAGRIVFHR"
     gene            complement(6281..6739)
                     /locus_tag="VF089_20135"
     CDS             complement(6281..6739)
                     /locus_tag="VF089_20135"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6176325.1"
                     /translation="MDRIETTGNRNDSSSFGSLISGILQDAQTLIAKEIAAAKLELKQ
                     EMSKGIKAGISLGIGGFLLVVGFILLTLMIVFLISTYTPIPLWGSLGIVGLVYCVAGA
                     IALIIGKRKVTEVKPVPEDALQHTKEDVRYIAERASSGFEKTELRKATQH"
     gene            7187..7507
                     /locus_tag="VF089_20140"
     CDS             7187..7507
                     /locus_tag="VF089_20140"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6176326.1"
                     /translation="MWYIVLSQSLPDKELDKQSHYEEHRQWLDDQHRAGRLLFSGPTT
                     DGSFGVYIMLAASLSEAEDIAAQDPHHRRGIRQMQVLEWRAHRAFRLNGPTIEALEQI
                     AMKP"
     gene            complement(7645..10065)
                     /locus_tag="VF089_20145"
     CDS             complement(7645..10065)
                     /locus_tag="VF089_20145"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX6176327.1"
                     /translation="MRIGLWIKFRNLLACAVLIAMLASTPAEESQAGSTAPSPAVTES
                     APTSTPSPAAIPLPEIATEAEAALARVRERMAEAPDSRTMPEVAQQFPLLTREIDARL
                     RQTRRILAQRPSIQTLSYLQSEWRPLRRNLSSWNQELTGYLGRLEGEMAQLDEIAKVW
                     RETLDAAKVANVPPEVLGRIQAVLAETEKARKEIERYSGRALTLQNRVATQDARISNA
                     LALIAQTRENALDRLFTPDSAPIWSIDLGSRRTQELRAESSDSITMQWYALSAYAARQ
                     TPSFLVHGVILIGIAAGFLWLRRRVQPRLEDTGIGCPAIVCETPMAAAFLLSMFFSRW
                     IYPQAPRLLWAFLGALALIPTAIILRKLIARELYPALYGLAAFYLVDQVRAVLVAEQL
                     VARLLLLGETLGFMAFLVWLLRSVDRLSTQNTSFGRLRKIVKIAAVLALITAGAAFIA
                     NATGYLTLASLLGNTLLSSGYFALILYAVVELLDSLVMIAFSLRPFRMLGIIAKHGVL
                     LRRRIYRILQWTAVLLWVLYTLNRLLLRQQLFRAISDFMTEELVVGALRISLSDIVVF
                     AVTVWAAVLVSRFVRFILDEEVYPRVHLTRGLPYAISSMVNYSIVLVGFFAAVAALGV
                     DMTKVTILAGAFSVGVGFGLQNIFNNFVSGLILLFERPVNVGDIVQVDDASGVVQRIG
                     IRASIIGTPAGAEVIVPNGKLISERVINWTFSNRRRSIELQLAVAQVTDPRRVIALLE
                     RAANEHPDITRDPPPKAWVVKLGPDSLVLELRAWTDRVHDWMEVRSEVAIAVSDKLAA
                     ESVQLRAV"
     gene            complement(10295..>10479)
                     /locus_tag="VF089_20150"
     CDS             complement(10295..>10479)
                     /locus_tag="VF089_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6176328.1"
                     /translation="GTFLTRERHRLEEMRPLFERKQAHPVIDTVLPLEEVGKAHDRLD
                     SHHGRGKIVLQVGSDR"
BASE COUNT         2467 a         2925 c         2816 g         2271 t
ORIGIN      
        1 ccgttggccg ccgcgggaat ctcagacagt aattcgttat cggcgtttga gaaaccgggc
       61 tcatagatat tcttgctcat ggctctaaca aatagctcca agtctccgtg agagtgtcgg
      121 tttcattccg taaaaatgcg cgcagctgca gcggcttgtc tttcggcggt ttaacttgaa
      181 aagcagcgcg ccagccgccc gtgatcggat tcttgaccgt actctgctga accaactgcg
      241 catcgccggc aacagtgacc actgctttca ccggagccgt gtccgggaga cttttgagct
      301 taccgccttc aaaatctaag atgattctct ttagatcatc tttatcgccg accccgacac
      361 gggtggcggt tacccggcct gacgtcgcag cgaggggttc ttcggtttga aagttaatcc
      421 tgtacgcgag atccagcggt tgacccaccg ccggcatgct gcgcgggatc cagtaagcca
      481 cgatgttatc gttggtttct ttttggctcg ggatttccac cagttttatc tgccccttag
      541 gccaggtacc tgaaggagtg acccaagcat tggggcgcat ctcgtgacgc gtttccagat
      601 cttcgtagtt cgaataactg cgatcccttt gcagcagacc aaagccgcgt agattgtcga
      661 agtcgaaata gctgacctgt agtctcgccg gatttataag cggccgccaa atccactctc
      721 cgctacccgc agccatcatg agtccgtcgg aatcatgcac ctcaggacgc cagtctgtgt
      781 tcggacgcgg cttctcctca ccatacagga acatgctggt gaggggcgca atccccaact
      841 ctttgaccct ctttctctcg aacaaccgag ctttcacatc gaccaaagtg cgatctcccg
      901 gtcgaacgat aaactcgtag gcgccggtta aactttgact atcgagcaag gctaaaattt
      961 taactgtgcg cgcctgagcg ctcggccgct cgagccaaaa ttcacgaaag gtcggaaatt
     1021 cctcgccggt gggcaaggcg gtgtcgatag ccagcccacg tgccgaaaga ccgaacacct
     1081 gcttcctcgc caccgcgcgg aaatagctag cgccggcgaa aacgatgaca tgattgtatt
     1141 cgttcttctt gtaaagcgga taggcaatgc ggaatccggc gaagccgagt tcaggcggga
     1201 tcttatcggc aaacttattc tttccataag agaattgttt cggagaaaaa gcaactttgc
     1261 ggacgccctg cgaatcaatc gtgttgatcg caacgctatg agtgtaaaat agaccgggat
     1321 gtatgaactg cacctgaaag ttgccgctgt ccttccacag gctttgcgca gtatcgaacc
     1381 ggatgtcgcg atagtcgtcg taactgattt gtttcagaaa ctccggtacc ggcgggggtg
     1441 gcttgaaggg ctctgccgcg agtttacgcg cggtctccgc aacctggtca aatccgaata
     1501 cgccgttggc aacggctaag gggcaattga acagaaaagt cagccagaca aaaacattgc
     1561 agcacagaat ccgtccccga tgacggaagg aacatggcgg tatcattttg gttctattac
     1621 acactccatc ggctgcgtcg ccggcatagc gcaatgccaa cgcgcccttt gaacattgat
     1681 ccaaaagcgt ttttaatcag gaaaagtatc ggcctggggg ctttatccta tcgatatgaa
     1741 cgcagaactc cagcgtgtca tcagagacag ctcgcgccta tgatctgcac cagcaactct
     1801 aaagcgtttc acgatgcaag ccggatgaga tcagcatact gttcgccgcg gctattctta
     1861 accaccttga tcaagatcaa gtcgccgtaa cgtaaatcgt caaccggcat ctcgcgttcg
     1921 cgattataga ccacagtggc cggatcatat ttaatgactg tcatccggcc ctcggtggtg
     1981 cggagctgga tttcgcggcg actctgatca accttttgca ctgttccagt cacttcatcg
     2041 gcttgtggcc gcggtatgtc agcacgggat tcgggacgcc ggtctaactc ccggcgatct
     2101 aaatcccggc ggctaagatc agcgcgatcg tcaatcgtcg gccgcggtaa aagcgcaata
     2161 ctctcacatc cgacggagga caccagaaac agcagcggcg cagctgctat acttttcctt
     2221 ctagacttct gcttcataat cgatatcccg ccgaatgaat tgagcaatta agatgccagc
     2281 agaggcatcg tagtttttac tacttgcgtg tatttccgct gaaacaacgc agaacgtggt
     2341 atcgatgatt tgacgcaaag ttgagacact caattgaaac cagcggcgca ttaagctgaa
     2401 aaagatgaat ggaagctgcc tccacctgaa gagaaatcca gtcaccaaaa cgcgcggcat
     2461 gagtgtggat cgcgtcagac aagaccaacg cttttcagtt ccacggttac tggaatggaa
     2521 attccgggtg tggctttact caattttttg cagttctttt tcttcgagga tttgaaccga
     2581 gtcgtcggcg agttttactt catataacgt tatgcctcgc gggtggatgt ctccggcaac
     2641 atgcttcggg aactcgccca cataaacccc gtcactgact ttaccgcgcg acgagttttg
     2701 attcttcaat acaaccgcat cgccttttct taattgaaac atgaacgcaa aactttcgtc
     2761 gagtcgcgga tcatcgattt tgatcgtctc catgaaagtt atcattgctg gtttttgccg
     2821 tttttcaagg ggtgatgcag atccggcgat cccgcgtgca gcacgcgcgg caggactagt
     2881 ggcatatgga ttgcgttttt tcatgcgaac agcatagtta cagatgtgct ggggtggcga
     2941 actatctagc agggtgctca aaaaccctct tgcaggctgc tcaaaaagat ccagatgcga
     3001 ggcgcgcgaa aatcgacgag cggaggcgta cttacagtgc gtcgcggcga ggcgattgag
     3061 cggcaacgaa gcatatgggc cttttttcag cagcctgtag cagtgagcag tgataagcca
     3121 gcagtaaatg acggtgcaac ggttgcacga tgaatacttt cgcgagatcg acactgttat
     3181 cgttgtcacg gaggagaaat tatgagagcc acttcgcaga ctaagctccc gctagcatta
     3241 aaaattgccg ctgccggcgt cgccgcagcc ggtttctcct gcgccttgcg ccgctaccgc
     3301 agaatcgatt tctccggcaa aaccgttctg atctcgggag catcgcgcgg gcttggtttg
     3361 gaattggccc gccgttttgc agcgcaacgc gccaatgttg tgttgatggc ccgcgatcag
     3421 gagcgactaa cagaagccgc tagagaactc gaagcctatg gcatcgaggt ctcgacccgc
     3481 cagtgtgatg taacccaacc cgaggaagcg cggcgcgcta tcagatcggt tatcgatgag
     3541 ttcaggtcaa tcgatgtact ggtcaataac gccggcatca tccaagtcgg acctgcggaa
     3601 aacatgaacc gtgacgatta tgccgacgca ttggcggtgc atttctgggc tccgctcaac
     3661 ctgacggcgg aagtgcttcc gtacatgaag gggcaaggca gtggccgcat cgtcaatatt
     3721 acctcaatcg gcggcaaggt cgcagtccct catctggcgc cctatgtcgc cagcaagttt
     3781 gctctggttg gcttttccga gtcgatgcgc gcggaattga taaaagacgg tatttacgtg
     3841 actacagtgg ttcctggttt gatgcgaacc ggctctcaca tcaacgcgtt tttcaagggc
     3901 cagcatcaaa aagagttcgc actcttcagc attgccaatg cgtcaccgtt attctcgaca
     3961 gcctcggaac gcgccgcccg gcagatcgtg gaagcgtgtc gttacggcaa agccgaactg
     4021 atcattacgc cgcaagcgcg ccttcttcac ctggctaatt ccttatttcc aaatattacc
     4081 gcggaagttc taggattgat ttcacggagt ctgccaagca cgcggcccgg cgaaggcaac
     4141 gctttgaaac gtggctggca aagccattct tttatggcac cgtcggtact tacccgcacg
     4201 gccgatagag ttatccgccg caaccaagaa cagccgcggt ctgcgccggg tacgaatcgg
     4261 tcaaacggat ttgccaaagg gtctgcgccc gagcgcgaca gaagtcggta gaatgcatct
     4321 cgcgaggcat ttgataacaa tacgatgacc gcggcaagtc agccgggcaa gaatagttgc
     4381 aaccgatcag gcgtttatga acggcagcta acgatgactg tcgaaaataa acctaaaaag
     4441 gcgaaagcag aaaagacaaa tcaatgtacc tgcactcacg gcacccattt cctaggccgc
     4501 tgccgcaagc gagtgaaacc tccggcgaaa caatgcacgg aatgccttca ggaccacaca
     4561 cccgtcaccc aggcgcctcc ttgatcgaaa cgctaaaaaa aatcttttaa cgcttgccga
     4621 gcggcaaaat aaccgcacat gccgtgcacc ccgccaccgg gtggcgtcga tgccgagcag
     4681 agatagagtc cgcgcgctga cgtagaatag agcttcagcg tcggacgtaa gaacagttga
     4741 ttcaaagttg gcgcgccgcc attgatgtcg ccgccgatca agttagggtt gcgccgctcg
     4801 agttcagcgg gcgcagccac gctgcgggca agaacggtat cacgaaaccc cggcgcaaag
     4861 cgttcaattt gagcttcaat gcgttcggtc atatcgaacg tggatccgtt tggcacgtgg
     4921 cagtacgccc atagcgtgtg ctttgtttca ggcgcccgcg acgaatcaaa tagagacggc
     4981 tgtaccgcga gcacgaaagg aatctctgtg tgatcgcctc gccaagtcgc gcgctccgaa
     5041 gctgctattt cctgcaatgt gccgcccaga tgaaccgttg ccgcgcgcgc gcatgccttc
     5101 gctttccaag gaaccggcgc ggaaagcgcc cagtcgattt tgaaagcagc catgccgtag
     5161 cgataacccc gaagccggcg gcggtagctc ggcgaaagtc gattgccggc aagtgccagc
     5221 agttgcttcg gcgtcacgtc gcacagaatg actctggagg aaggaagctc atccagagtt
     5281 cgcaccggca cgttggtata gatctcgccg cccttcgatt ggagatacaa agcgagcgcg
     5341 tctgccagcg attgtgagcc tcgccgtgcg atcggccaac ccagcacatg tccggtcgtc
     5401 gccagcacca ggccgaacgc cgctgaaccc cacgcctcta aaggcaacat tgaatgcgcg
     5461 gatagaccgg caaagagcgc tctggcgcgt tgtcccttaa agcagctctg actaaagctc
     5521 cgcgccgggc ggattgccga gaaaccgaat cgcgccaacg ccaggggctt acgcggccat
     5581 ctaagcgggc cgagcaaatc gctatcgagg ccgtgccatg actccgcgag aggctgcatc
     5641 acccgtcgat acgcagcccc gtctccaccg agcccggcgg ccgtgtcgtg caacgacctt
     5701 tcaagtagga cggccgagcc atcgtcaagc gcatgagcga gcggcgatgg cggctgtacc
     5761 cattccagcc catatcgagc cagaggcaat gaacgaaaca gcggtgagcc gatggccaat
     5821 ggatagactg ccgagcagac gtcgtggacg aatccaggca aggttaattc cgcggagcgc
     5881 attccgccgc cgatcttgtc gcctgcttca taaaccgcca cggatctacc gctatcaatc
     5941 attgtgatag cggccgccag cccatttggg ccggagccta taacgatagc atcgtatcgc
     6001 tgcgactgag ccattcagcc ccggctgagg tcggcagcat tgagaaccgg cggttgtgag
     6061 ttcaagcgga aaggattttt atgcgtgaca tgcgacgaga tcccgtcggg gttctatggc
     6121 acgttggcag cttcgacatg atctttgcac gccggacact tctcttctcc ggcatgcata
     6181 acgtttccac agcccttcgg gccatgactc gggtgatcac agggacaatg agtcttgagt
     6241 tggtcggcga catcgttgcc ggtcggatcg tatttcatcg ctagtgctgg gtagcttttc
     6301 taagctcggt tttctcaaat ccggaggagg cccgttcggc aatatatcgg acatcttcct
     6361 ttgtatgctg tagcgcatct tccggcaccg gctttacctc ggtaactttg cgcttgccga
     6421 ttatcaatgc aatagcgccg gctacgcagt agaccagccc gactatgccg aggctgcccc
     6481 aaagcggtat tggtgtgtag gttgaaatca gaaatacgat catgagagtc aacaagatga
     6541 atcccaccac cagcaaaaag ccgccgattc cgagtgagat gccggccttg atgcccttgc
     6601 tcatctcttg cttaagctca agcttggctg ccgcaatttc tttggcaatc aaagtctgag
     6661 catcttggag gatacctgaa atcaaggatc caaatgagct gctgtcgttt cggttcccag
     6721 tggtttctat tcggtccata cgagtctcct tggtcattta acgatttggt tcggcgcaac
     6781 ttttatgcca ttgccttgag aaaatcaccc ttgcgccaac attcatcgtg agtattctct
     6841 ttccgtcacg gaatctctcg tctatcgatc gaatgcggca aacacgagag attagctgtc
     6901 accaacccgg gacactggaa catcagttgt ttcacaagtg gagcaaggtc ttggcactac
     6961 tgttggaacc cttcctacgc gtcttatcac ttgtcggaaa caattaagac tggaattagc
     7021 atgtatataa aactgcaagg gaggattgat gattcatctc ctagtaattc acccccacga
     7081 aacaagatca gcattcacta gaggagacaa cccgcctact ctaagagcgt cgatttatat
     7141 gagcgcttga gccattatcg ttcgaactga aaaacggagg tcaagcatgt ggtatatcgt
     7201 cctttcccaa tcattgcccg acaaagagct agacaagcaa agccattacg aagagcaccg
     7261 gcagtggctg gatgatcaac atagggctgg aagattgctt ttttcgggac ctactacaga
     7321 cgggtcattc ggggtctata ttatgctggc ggcgagtctc agcgaagcag aggacatcgc
     7381 agctcaggat ccgcaccacc ggcggggtat tcgccagatg caagtgttgg aatggcgagc
     7441 ccatcgcgcg ttccgcttga acggaccgac gatcgaggct cttgagcaaa tcgccatgaa
     7501 gccctagctc attgagaccg gccaaagctc cacggaaaag ccaggagaga tttattcgag
     7561 ccgcaactgt acctgggcgg tacggcagct ggccaggcaa gtgcgggtca catcgcaggg
     7621 cagcggcgac attgcaaaat ttctctaaac cgcacgcaac tgtacacttt cagccgctaa
     7681 tttatcgcta accgcaatcg caacctcgct gcggacttcc atccagtcgt gtactcgatc
     7741 ggtccaggcg cgcagttcca gaacgagcga atccggtccg agttttacaa cccatgcctt
     7801 gggaggagga tcgcgcgtaa tatccggatg ttcgttggcg gcgcgctcaa gtaaggcgat
     7861 gactcgacgc ggatcggtga cctgcgcgac cgccaattgc agctcaatac tgcgccggcg
     7921 gtttgagaac gtccagttga tgaccctttc cgatatcagt ttgccattgg gtacgatcac
     7981 ctcggcgccg gctggcgtgc cgataatgct cgcacgaata ccgatccttt gaaccactcc
     8041 cgaggcatcg tcgacctgca cgatatcgcc gacgttaact ggccgctcga ataacaaaat
     8101 gagacctgag acgaaattgt tgaaaatgtt ctgcaagcca aacccaacac cgacgctgaa
     8161 agcgccggcc aggatcgtaa ctttcgtcat atccacaccc aacgccgcta cggctgcaaa
     8221 gaatccaacc agcacaatgg aataattaac catgctcgaa atcgcgtaag gcaaaccgcg
     8281 ggtcaaatga acgcgtgggt agacctcttc atcgagaata aaacgaacga accgtgaaac
     8341 cagaacagct gcccaaaccg tgacggcaaa aacaactatg tcgctcagcg aaattcgcag
     8401 tgcgccgaca accagttctt cggtcataaa gtcgctgatc gccctgaaca gctgttggcg
     8461 caacagcagg cgattgagcg tgtaaagaac ccacaggaga accgcggtcc attgtaagat
     8521 ccggtaaatt cgacggcgga gcagtacgcc gtgtttggcg atgatgccga gcattctgaa
     8581 cggtcgcagg ctgaaggcga tcatcaccag gctatccagc aactcgacca cggcataaag
     8641 aatcaacgca aaatagccgc tgctcagaag cgtgttgccg aggaggctgg cgagagtcaa
     8701 gtagcccgtg gcgttggcga tgaaagccgc cccggccgtg atcaaagcca gaaccgccgc
     8761 gatcttgacg attttgcgca gtcggccaaa gctggtgttc tgggtgctca atcgatcaac
     8821 tgagcggagc agccaaacga gaaacgccat gaagcccaac gtttcgccga gcaaaagtag
     8881 gcgagctacc aattgttcgg caaccaaaac tgcgcggacc tgatcgacaa gataaaacgc
     8941 tgcaagaccg tacagtgccg gataaagctc gcgtgcgatc agcttgcgca agatgatcgc
     9001 ggtggggatt agggccaggg cgccgagaaa tgcccacaac aaccgcggcg cctgtgggta
     9061 gatccagcgg ctgaaaaaca ttgacaggag gaaggcggct gccatcggtg tttcacacac
     9121 aatggctgga cacccgatgc cggtatcttc aagccgcggc tggactcgtc ggcgcaacca
     9181 tagaaagccc gcggctatac caatgagaat taccccatga accaagaagg acggcgtttg
     9241 gcgcgccgca taggcgctta gtgcgtacca ttgcatcgta attgaatcgc tgctctcggc
     9301 tctaagctcc tgggtacggc gcgaacccag gtcgatgctc cagatgggtg cgctgtccgg
     9361 cgtgaaaagg cgatctagcg cgttttcgcg tgtttgtgcg atcaaagcca gggcattgct
     9421 aatgcgagca tcttgcgtcg ccacccggtt ttgcaatgtc aaggcacgcc cgctatagcg
     9481 ctcaatctcc ttgcgggcct tttcagtttc ggcgaggacc gcctgaattc gaccgagaac
     9541 ttccggcggg acgtttgcga ccttggccgc atcgagagtt tcgcgccaaa ctttagcgat
     9601 ttcgtcgagc tgggccattt caccttcgag ccggcccagg tagccggtga gttcctgatt
     9661 ccagctgctg agattgcgcc gcagcggacg ccattccgac tgtagatagc taagcgtttg
     9721 gatcgatggt cgctgcgcta aaatcctgcg agtctgacga aggcgcgcat cgatctcccg
     9781 tgtcagtagc ggaaactgct gtgccacctc gggcatcgtc cggctgtcag gagcctcagc
     9841 cattcgctca cgcactcgcg ccagggctgc ctcggcttcc gtcgcgatct cgggcaatgg
     9901 aatcgctgcc gggctgggcg tactggtcgg agcggactct gtcactgcgg gagacggcgc
     9961 agtggaacct gcctggctct cttcagcagg agtcgaggcg agcatcgcga tcagcaccgc
    10021 gcatgccaga agatttcgaa atttaatcca caatccaatt cgcataatca attaccgcgt
    10081 ctggacgcag gtgcgcgctc gttcctaaaa tgccgcgctc gttcggaatt ccgactagca
    10141 ggctgctgaa aaagacccat atgcttcgtt gccgctcaat cgcctcgctg caacgtactg
    10201 taagtacgcc tccgctcgtc gatttttcgc gcgcctcgca tctggatctt tttgagcagc
    10261 ctgcaagagg cttttttcag caccctgcga gagattaacg atcgcttccc acctgcaaaa
    10321 caatctttcc tcttccgtga tgtgaatcga gacggtcatg cgccttacca acctcctcta
    10381 gcggaagcac cgtgtcgatc accggatggg cttgttttct ttcaaacaga ggccgcatct
    10441 cttctaaacg gtggcgctcg cgcgtcagaa aggtgccat
//
LOCUS       DASUTG010000862         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2209661, whole genome shotgun sequence.
ACCESSION   DASUTG010000862 DASUTG010000000
VERSION     DASUTG010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209661"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1525
                     /gene="tilS"
                     /locus_tag="VF089_20155"
     CDS             152..1525
                     /gene="tilS"
                     /locus_tag="VF089_20155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX6176329.1"
                     /translation="MLSPGQRILAAVSGGPDSVAMLYLLYDLREQLELTIEVAHLQHG
                     IRGEEAVEDARFVACLAEQLGLIFHLKEIDLPQLKIASRKGSLEEIGRLERYLFFGDT
                     ARRRQLDAVATAHTADDQAETVVMRLFRGAGRTGLRGMAPVRPFELPNGVSGNVLLIR
                     PLLDATRKQVTEFLDQRKVIYRTDSSNADLFYLRNWIRLKLMPEVSQKFGAELPARLC
                     AQAEVLHDEEDYLGDLTRQQFENVSDGKDLNRGAFLNINKALQRRVIRLWIERRRGHL
                     RAVGFDHIEAALSLIAAGPPQGRLAFPGGWQLAREYESITFERDRPNVKPRCYSYQFK
                     PGMTLAVIEAGITIDSELIDAVPAKLPNNFMEAVFDAGLLKGNLLVRNFRNGDRFRPL
                     GMEGHKKVKDLLIANQVPLHTRTALPLLVLDDEILWIPGYGRSEFARVGSATKISLHL
                     KAIPQPK"
     gene            1597..3435
                     /gene="ftsH"
                     /locus_tag="VF089_20160"
     CDS             1597..3435
                     /gene="ftsH"
                     /locus_tag="VF089_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6176330.1"
                     /translation="MSQSSKHIALWLVLLLVLLGIFSVFSKQAGRDPEVVFSEFLLAV
                     ERGEVQEVIIQGHNIQGKYKNGERFRTFAPDDPELVKSLREKKVKIAAKPEDDSPWYY
                     VLLINWFPMLLLIGVWVFFMRQMQVGGGKAMSFGKSRAKLLTENQHRVTFSDVAGAEE
                     AKDDLQEIIAFLKDPKKFTRLGGRIPKGCLLVGPPGTGKTLLARAIAGEAGVPFFSIS
                     GSDFVEMFVGVGASRVRDLFVQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRRVVVPRPDVKGREGILQV
                     HTRKVPVASDVDIAVLARATPGFAGADLENLVNEAALLAARNNKDKVDMRDFELAKDK
                     VMMGAERRSMIISDEEKRITAFHEAGHALVAKLLPGADPIHKVTIIPRGMALGLTQQL
                     PMDEKHTYPKEYLLNNLVILFGGRVAEELVLNHMTTGAGNDIEKATELAHRMVCEWGM
                     SEKLGPMTFGKKEEEIFLGRDFTQTADYSESTAIEIDSEVRRIIQESYHKAKDLLKTN
                     LALLHKVAESLLEREVLDGSEIDAIVKDFSGNGGDPMNPTRAAAVA"
     gene            complement(3666..>3853)
                     /locus_tag="VF089_20165"
     CDS             complement(3666..>3853)
                     /locus_tag="VF089_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176331.1"
                     /translation="PSRYNSPWLTIGSSAKISDGIHIKKTIDGVLLTQITQLRLTQYR
                     FRSGNRLGYNFRRCSIP"
BASE COUNT          904 a          944 c         1095 g          910 t
ORIGIN      
        1 ggagaaaacc gtctgtttcc aacgtcacta gatttcctaa gcgtcgtcga gtccggcgag
       61 cttttgttct tgttcaaatg cagaaactgc acagactctc ttaaacatgg acttgccgag
      121 aaaatttaaa caaacgatcg agaaacaccg aatgctatct cctggccagc ggattttagc
      181 agccgtctcg ggcgggccgg actctgtagc gatgctttat cttttgtatg atctgcgcga
      241 gcagcttgag cttacgatcg aagttgccca tctacagcat ggaattcgcg gcgaagaagc
      301 cgttgaggat gctcggtttg ttgcttgctt ggcggagcaa ttgggcttga tctttcatct
      361 aaaagaaatc gatctgccgc aactgaaaat agcttcacgt aagggtagcc tggaagagat
      421 cggtcgattg gaaagatatc ttttctttgg cgacacagcc cgtcgacgtc aactcgacgc
      481 cgtggcaacg gcccataccg ccgacgatca agccgagacg gttgtgatgc ggctatttcg
      541 tggcgccggc cgaaccggcc tccgtggcat ggcgccggtg cgtccgtttg agcttcccaa
      601 tggcgtctct ggaaatgtgc tgctgatccg gcccttgctt gacgccactc gaaaacaggt
      661 gacggaattc ctcgaccagc gaaaagtgat ttatcgtacg gatagcagca atgcggacct
      721 gttctatttg cgcaactgga ttcgtctaaa gctcatgccg gaagtgagcc agaagttcgg
      781 cgctgaactt ccggcaaggc tatgcgccca ggccgaggtg ctgcatgacg aggaagatta
      841 tcttggcgac ctgacccggc agcagttcga aaacgttagc gacggaaaag acttaaaccg
      901 tggggccttt ctcaacatca ataaggcgct tcagaggcga gtgattcgcc tgtggatcga
      961 acggaggcgc ggtcacttgc gggccgtagg cttcgatcat attgaagcag cgctatcttt
     1021 gatcgccgcc gggccgcctc agggacgttt ggcttttccc ggcggctggc aattggcacg
     1081 cgaatacgaa tcgatcacct ttgaaagaga tcgtccgaat gttaaaccac gctgttatag
     1141 ctaccaattt aaacccggga tgactttggc tgtaatagag gccggcatta cgatcgatag
     1201 tgaattgatc gatgccgtcc ctgcaaaatt gccaaataac tttatggaag ccgtattcga
     1261 tgccggttta ttgaagggaa acctgctggt gcgcaatttc cgtaatggtg atcgatttcg
     1321 gcctttgggg atggagggac ataaaaaggt caaagacttg ctcattgcca atcaagtgcc
     1381 gttgcataca cggacggcat tgccgttgct ggtcttggat gacgagatcc tgtggattcc
     1441 cggatacggc cgaagtgaat tcgcccgggt cggatcggcg acgaagattt ctctgcattt
     1501 gaaggcaatc cctcaaccta agtaggaaaa ctgtatattg atctaactgg ttgcgtgtgt
     1561 tagtcttttc tggttcttga aatcggagga ttcgggttga gtcaatcatc aaaacatatt
     1621 gctctctggt tggtgctgct gttggttctt ctgggcatct tcagcgtgtt tagtaagcag
     1681 gccggccgcg atcctgaagt tgtttttagt gaattcttgt tggctgtgga gcgtggagaa
     1741 gttcaggaag tcattattca agggcacaac atccaaggca aatacaagaa cggcgaaagg
     1801 ttccgcactt tcgcgcccga tgaccctgag ttagtcaaga gtctgcgcga gaagaaagtc
     1861 aagatcgccg caaagcccga ggatgattcg ccctggtact acgtgctgct gatcaactgg
     1921 tttccaatgt tactgctgat aggtgtctgg gtcttcttca tgcggcaaat gcaggtcggc
     1981 ggcggcaagg cgatgtcgtt cggcaaaagt cgcgcgaaac tgctgacgga gaatcaacac
     2041 cgggttacat tttcggatgt agccggcgca gaggaagcta aggacgacct tcaggaaatc
     2101 atcgcatttc ttaaggatcc caaaaaattt accagactgg gtgggcgcat tcctaaaggg
     2161 tgcttgcttg tgggtcctcc aggcactggg aagactttgc ttgctcgagc catcgcaggc
     2221 gaggccggcg tcccgttttt tagcatcagc ggctcggatt tcgttgagat gtttgtcggt
     2281 gtgggcgcgt cacgcgtacg cgatctcttt gtgcaaggta agaaaaacgc gccttgtatt
     2341 atcttcatcg atgaaatcga tgccgtggga cggcatcgcg gcgccgggct cggcggcggc
     2401 catgatgaac gggagcaaac cctcaatcag ctgttggtgg aaatggacgg gttcgaagcc
     2461 aatgaaggcg ttatcctgat tgccgcgacc aaccggcccg atgttttgga cccggcgctg
     2521 ttgcggcctg ggcggtttga ccgccgcgtg gtggtgccac gtccggatgt aaaaggccgt
     2581 gaaggcatct tgcaggtgca tacacgcaaa gttccggtcg cctccgacgt cgacatcgcg
     2641 gtgctggcgc gcgcgacgcc gggctttgcc ggcgcagacc tcgagaactt ggtcaatgaa
     2701 gccgctctgc tcgccgcgcg caataacaaa gacaaagtcg atatgcgcga ctttgagttg
     2761 gccaaagaca aagtgatgat gggcgccgag cgccgcagca tgatcatcag cgatgaagaa
     2821 aagcgaatta ccgcatttca cgaggcgggc cacgcgttgg ttgcgaaact gctgccgggt
     2881 gccgatccga ttcacaaagt caccatcatt ccccgcggca tggccttggg tttgacccag
     2941 caattgccga tggatgaaaa gcacacgtat ccgaaagagt atttgctcaa caatctggtc
     3001 attctttttg gtggtcgagt cgctgaagaa ctggtcctca accatatgac caccggggct
     3061 ggaaacgata ttgaaaaagc caccgaactc gcgcaccgga tggtctgcga atggggcatg
     3121 agcgaaaaac tcggtcccat gactttcggc aagaaagaag aggagatctt tttagggcgg
     3181 gacttcacgc agaccgctga ctactctgag agcacagcca ttgagattga cagtgaggtt
     3241 cgacgcatca tccaggagag ttatcataag gccaaagatc ttctcaaaac gaatctggcg
     3301 ctgcttcaca aagtcgccga atcgcttctt gaacgagagg ttttggacgg ctcggaaatc
     3361 gacgccattg ttaaagattt tagtggcaac ggcggcgacc ctatgaaccc gacccgggcg
     3421 gcagccgttg cataagtcga gttaaacact ctaccctcag tcttatcatt gacatagcag
     3481 gatcgtttcg tgagggcatg aaacgatcct gattctttca cctgtggtca cgttctttgc
     3541 gactagggtt ttcgccaacc cggatttcac agtaacgagt gctctacctg ggagcggtgt
     3601 gccagaagca gcgaggcact gaacgaatga gctcatcttg gatcctgctt ggtgcagggc
     3661 tgatactacg gaatagaaca gcgacggaag ttatacccga gccgattgcc tgagcgaaac
     3721 ctatactgtg tcaatctgag ctgcgtgatt tgggtgagca aaacgccatc aatagttttc
     3781 ttaatgtgaa ttccatcaga gatcttagcg ctgctgccga tcgtcagcca gggcgagtta
     3841 tatctactcg gca
//
LOCUS       DASUTG010000863         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1169082, whole genome shotgun sequence.
ACCESSION   DASUTG010000863 DASUTG010000000
VERSION     DASUTG010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169082"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1047
                     /locus_tag="VF089_20170"
     CDS             37..1047
                     /locus_tag="VF089_20170"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176332.1"
                     /translation="MWRILTRIFAIVFLFAIQPDNAFSQARPKIVVGYASMSTVATTL
                     WVAQERGFFAKHGLDAQAIFIPGSPTLIASLSTGDVHFGYTGGTATLGAAVGGLDIKM
                     IAAFANHVQTDLVVRPEIKTPADLRGKRIGVTSIGGTGWMSAMLGLEQIGLNAEREKI
                     LLAAFGDQRVITQALETGTIQGGSLAGVFGQRLKRAGYHFMGELEKIPLVGTSIVVKS
                     DYLAKQQSIARNTLRALIESHAFIHNPANKAAVIEITSRKLGITDPVAANDGYEDYVR
                     RSDRNPSISVEGLKNIQRFMKLRNPKIADVNLDRLIDESLLRDLGKSGFMEQSFGGTA
                     IR"
     gene            1093..2184
                     /locus_tag="VF089_20175"
     CDS             1093..2184
                     /locus_tag="VF089_20175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176333.1"
                     /translation="MAIDGMKVIDLDSHLVGDLESWEQTVEAKYKEFLPRKLPTKDNE
                     RRKTLVGNQIMIGSELGRQKAEKKEWVTPQDLTAQGRVRNMDLDGIDVAVLSPNSPAL
                     DILWFVDDPELAAAYARAQNNYMNYYASQQPGRLMWAGVIPLQDPDEAIKELHRSREL
                     GSKALNVKATPIPGKEWWDPHFDPIFHELEKTNTPIIFHDTKTGSMGHERFADNFFFS
                     HMVGRTIESMVCLMIYLCGGVMEKHPNLKIICLETGASQMPWWLSRMDEHWEKLPHLV
                     PWQKRKPSDIFNESVYVGCEPFEDGLFEWAVEYLGGDRLVLATDSPHWDSSQPGQVTG
                     PVAKSTKISQDNKRKVLGENAINLLGLGL"
     gene            2276..3289
                     /locus_tag="VF089_20180"
     CDS             2276..3289
                     /locus_tag="VF089_20180"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176334.1"
                     /translation="MVSRLVAVLVISIFFSAPGRAYAAAAGKQVVIAHAAMNARVAPL
                     WVARERGFFAKYGVEADTIFVRGAPTLVAAMQSNEIDVGYTGGTAVVGAVANGADLKV
                     LSAFTNRITYDLMVKPGIKRPEDLRGKIFGIQSIGGTVWMGAILALEHLKLDAERDKI
                     NIIAVGDQNVLAQALETGTIDATVLDGVQSRRLNALGFPTLADLNKANLPILSSGFVM
                     REPNLQKNPQLAENLMKAVIEGAAFSLSMSQKPSTLKILQKYLRITEKDAEEGYRDLV
                     LGTDKKPYAAPAGVTNVIRLMKRSNPKVEKVKPEQLIDDRILKKLDQSGFIDEILAKY
                     GVK"
BASE COUNT          809 a          834 c          953 g          756 t
ORIGIN      
        1 cggttcgaca cttggattta ggaggtagtc acgtaaatgt ggcgcatttt gactcgaatc
       61 tttgcgattg tttttctttt tgcgatccaa cccgataacg ccttcagtca agcccgaccg
      121 aaaattgtcg tcggctatgc atcgatgagc accgtggcga cgaccctgtg ggtcgcgcag
      181 gagaggggat tctttgccaa acacggcctg gatgcgcagg cgatttttat tcctggatca
      241 ccgactctga tcgcgtcgct cagtaccggt gatgtgcatt ttggctatac cggcggcaca
      301 gcgaccttgg gagcggccgt gggcggcctg gacattaaga tgattgccgc ttttgccaat
      361 catgttcaga ccgatctggt ggtgcggcca gagatcaaaa ctcccgccga tttgcgcggc
      421 aagcggatcg gcgtcaccag catcggcggc accgggtgga tgtcggcaat gctcggactc
      481 gaacagatcg gcttgaatgc cgagcgcgaa aagatcttac tggctgcttt cggcgatcag
      541 cgggtgatca cacaagcgct ggagaccggc actattcaag gcggatccct cgccggtgtt
      601 ttcggtcaac gtctgaaacg cgcgggctat catttcatgg gagaactaga aaaaattccg
      661 ctggtcggaa ccagcatcgt cgtcaaatcg gattatcttg cgaagcagca gtcgattgcg
      721 cgtaatactc taagggcgct gatcgaaagc catgctttca tccacaatcc agccaataag
      781 gcggcggtta tcgaaatcac ctcgagaaag cttggcatca ccgatccggt tgctgccaat
      841 gatggttatg aagattacgt gcgtcgcagc gaccggaacc cttctatttc ggtcgaaggg
      901 ctcaagaata ttcaacgctt catgaagttg cgcaacccca agatcgccga tgtcaatctc
      961 gatcgcttga ttgacgagag tctcttacgc gatctcggca aaagcggttt catggagcaa
     1021 tcatttggtg gcacggccat tcgttgatca gtaagttatc tctactgacg attcgcaaag
     1081 ggaggaagca aaatggcaat tgatggaatg aaagtgatcg atctcgacag ccacctggtg
     1141 ggtgatttgg agagttggga gcaaacagtc gaagcaaaat acaaagagtt cttgccgcgc
     1201 aaactgccga cgaaagacaa cgaaagacgt aagactctcg tcggcaatca aatcatgatc
     1261 ggctccgagc tcggccgaca gaaggcggag aaaaaggagt gggtcacgcc gcaggatctc
     1321 accgcccaag ggcgagtgcg caacatggat ctcgacggca tcgacgtcgc cgtgctctcc
     1381 cccaactcac cggctctcga tattttatgg ttcgtcgatg atccggaact ggcggctgct
     1441 tatgcccggg cgcagaataa ttacatgaac tactacgcat cgcagcaacc gggccggcta
     1501 atgtgggccg gcgttattcc tctgcaggat cctgacgaag caattaagga attgcaccgg
     1561 tcgcgggaac taggtagcaa agcgctcaac gtaaaagcga ctccgattcc aggcaaagaa
     1621 tggtgggatc cgcacttcga tcccatcttc cacgagctgg agaaaaccaa cactccgatt
     1681 attttccatg atactaagac cggttcgatg ggccacgagc gcttcgctga taattttttc
     1741 ttctcgcaca tggtcggccg caccatcgag agcatggttt gtttgatgat ctatctttgc
     1801 ggcggcgtga tggaaaagca tccgaatctg aaaatcattt gcctcgaaac cggcgcttca
     1861 cagatgccgt ggtggttatc acgcatggat gagcattggg agaagctgcc acacctggtt
     1921 ccttggcaga agcgcaagcc cagcgatatc ttcaacgaga gtgtgtacgt cggctgcgag
     1981 ccattcgaag acggactgtt tgaatgggcg gttgaatatc tcggcggtga ccggcttgtg
     2041 ctggcaaccg attctcctca ttgggattca tcacagccag ggcaggtgac cggtcccgtg
     2101 gccaagagca ccaagatttc ccaagataac aagcgcaagg tcttgggcga aaatgcgatc
     2161 aatcttttgg ggctcggtct ctaattaact gcgacgacca ctggagttga gttgccgata
     2221 gggtaaaggc ggagcaagtc agcctaagtt tctatgctta aagaaaggag attgtatggt
     2281 tagccggtta gtcgctgttc tcgttatctc aatcttcttt agtgctccag gacgagccta
     2341 tgccgctgcg gcaggcaagc aggtggtcat tgctcatgcg gcgatgaacg ctcgagtcgc
     2401 accgctctgg gttgctcgcg agcgcggctt ttttgccaag tacggagtcg aagcggatac
     2461 catctttgta cgcggcgcgc cgactctggt cgcggccatg cagtcgaacg agattgatgt
     2521 gggttacacc ggaggcacgg cagtggtcgg ggcggtggcc aacggcgccg atctgaaggt
     2581 tctttcggcg tttaccaacc gcatcaccta cgatctgatg gtcaagccgg ggatcaaacg
     2641 gccggaggat ttgcggggta agatattcgg gattcaaagt atcggcggta ccgtttggat
     2701 gggagctatt ctggcgttgg agcacttaaa attggacgcc gagcgtgaca agattaatat
     2761 catcgctgtc ggcgatcaga atgtcctggc gcaagcgctg gaaacaggca ctatcgatgc
     2821 gacggtgttg gacggtgtgc agagccgtcg cctgaacgcc ctgggttttc cgacgctcgc
     2881 cgatttaaac aaagcgaatc tgccgatcct gagctccgga ttcgtcatgc gagaacccaa
     2941 tttgcagaaa aacccacagc tggcggaaaa tctgatgaag gcggtcatcg aaggggctgc
     3001 gttttcgcta tcgatgtcgc agaaacccag cacactaaaa attttacaaa agtatcttcg
     3061 aattactgaa aaggacgctg aagaaggcta tcgggatctg gttttgggga cggacaagaa
     3121 accctatgct gcgcctgcgg gtgtcacgaa tgtcattcga ttgatgaaac gtagtaatcc
     3181 gaaggttgaa aaagtcaaac cggaacaact gatcgatgat cggattctta aaaagctcga
     3241 tcagagcggc tttatcgacg agattttggc caagtacgga gtgaagtaaa cgagcaggtg
     3301 aaagcatccc aactgcttcc ttgcgcttgt tcgcctcgct ccaacagact cg
//
LOCUS       DASUTG010000864         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1467578, whole genome shotgun sequence.
ACCESSION   DASUTG010000864 DASUTG010000000
VERSION     DASUTG010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467578"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1520)
                     /locus_tag="VF089_20185"
     CDS             complement(18..1520)
                     /locus_tag="VF089_20185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6176335.1"
                     /translation="MNYSGKAYRYVAATISLTAGFLFLTPVRAQDSAPSTPTSKVERK
                     NQAPVSSEILRVKLPKPVEAKLKNGLTVLISEDHRFPAISVQLQISAAGALYEAENVA
                     GLASVTAQMLREGTKSRDSKQIAEEADKLGSSFGASAEFGSSVTTFFASGLSDNFDSW
                     FPLALEVLLYPSFPERELDNLKQRLGVQLRQQRAAPGFLASERFNRALFGQHPAAIVS
                     ATAESLESLTPAVLAKWHRERYVPQNAILGFAGNVRAGELIPKLEKWLADWDATSLQE
                     TLPANPVPSTERKVYLVDRPNSVQTTIALGNIAIDRRSPDYVPLVVMNHILGGGAAAR
                     LFLNLREEKGYTYGVYSDLSALRYPGPWRAGGSMRTEVTGQAVSEFFNEIRRIRDEKV
                     SDKELADAKRAITARFALSLEQPTGVLAFAMVQKTYGFPADYWDNYPGQIMAVTAEDL
                     QRVARTYLDPGTMQLIAVGDAEKIKPALDKYGPIEVYDSSGRLAPISKTD"
     gene            complement(1522..>2493)
                     /locus_tag="VF089_20190"
     CDS             complement(1522..>2493)
                     /locus_tag="VF089_20190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6176336.1"
                     /translation="EADRMRSLNINQANLDNQRHTVQEERRQGVDNRPYGRTYEAVIG
                     TAYDNFAYKHSTIGSMEDLNAATVKDVAEFFRTYYAPNNAVLSLVGAFKTEEAMALIK
                     KYFENIPSQPPPPVPNVAEPEQQAERRKTIDDPFAQTPRLDIVYKIPPGDTPDSYALQ
                     VLGQVLSGGLSSRLYQKFVKDKELALSISAGPDERRGPSLFWVSVMPRPGINLDEIEK
                     LVYQDLERTKTEAVMDWEIEKVHTQVRRQRAQGFYSTRSRANTLGYYAVYYNDPGLIN
                     SVQEKIFEVSKNDLQRVATKYLKESNRTVVTTLPKAKAAAPTATIHR"
BASE COUNT          531 a          681 c          688 g          593 t
ORIGIN      
        1 tgaactgagt cttgttgtta atccgttttc gaaatgggcg cgagtcttcc actgctgtcg
       61 tagacctcaa tgggcccgta tttatccaga gctggtttga tcttctccgc atcgcccacc
      121 gctatcagct gcatcgtgcc cggatctaga taggtacgcg ccacacgctg cagatcttcg
      181 gcagtcactg ccatgatttg accaggatag ttgtcccagt aatctgcagg aaagccatac
      241 gttttctgca ccatggcaaa agccagcacg ccggtcggct gctccagcga cagcgcaaag
      301 cgcgcggtga tagcgcgttt ggcatcagct aattctttat cggaaacctt ttcgtcgcga
      361 atgcgacgga tctcgttgaa gaattcagac acggcttggc cggtaacttc cgtgcgcata
      421 ctgccgccgg cgcgccatgg gccggggtaa cgaagcgcgc tcagatcgct gtagactccg
      481 tacgtgtagc ctttttcctc gcgaagattc aaaaacagcc gggccgcggc gccgccgccg
      541 agaatgtgat tcatgaccac cagcggcaca tagtcgggac tgcggcggtc gatggcgatg
      601 ttacccagag cgatggtcgt ttgcaccgag ttgggacgat ccaccaggta aactttccgc
      661 tctgtgctgg gaaccggatt ggccggcaaa gtttcttgca aactggtcgc atcccaatcg
      721 gccagccact tctcgagctt ggggatcaat tcgccagcgc gtacgttgcc ggcaaatccc
      781 aaaatggcat tctgcggcac atagcgctcg cgatgccact tggccaaaac tgccggtgtt
      841 aatgattcga gcgattctgc tgtcgctgaa acaattgcag ccggatgctg gccgaaaagg
      901 gctcggttga aacgctcgct ggcgagaaaa ccgggagccg ctctctgctg gcgcaactgc
      961 actcccagcc gttgcttgag attgtctaac tcgcgttccg gaaaactagg ataaagcaag
     1021 acctctagcg ctagaggaaa ccagctatcg aagttatcgc ttaacccgga ggcgaaaaaa
     1081 gtagtgaccg aggagccgaa ctccgccgat gcgccaaaac ttgatcccaa tttgtcggcc
     1141 tcttcggcga tctgtttgct gtcgcgcgat ttggtcccct cgcgtagcat ctgcgctgtg
     1201 acactcgcca gcccggcaac attctcagct tcgtacaagg ctccagcggc gctgatctgc
     1261 agttgaaccg atatcgccgg aaagcgatga tcttcggaga tcagcacggt aaggccattt
     1321 ttcaatttcg cttctacagg tttgggcagc ttgactcgaa gaatctcgct cgataccggc
     1381 gcttgattct ttctttcgac tttactggtc ggcgtgctgg gcgcggaatc ttgagcgcgg
     1441 acgggagtta ggaatagaaa accagccgta aggctgatgg tggccgcgac ataccgataa
     1501 gctttaccag aataattcat atcagcgatg gatcgtcgcg gtgggtgcgg ctgctttggc
     1561 ttttggcaac gtcgtcacga ccgttcgatt gctctccttc aaatacttgg tggcaacacg
     1621 ctgcaggtcg ttcttggaga cctcgaaaat cttctcctga accgaattga tcaatcctgg
     1681 gtcgttgtaa tagacggcat aataacccag ggtattggcg cggctgcgcg tgctgtaaaa
     1741 accctgcgcc cgctggcgac ggacctgagt gtgtaccttt tctatttccc aatccatgac
     1801 agcttcggtc ttggttcgtt ccaggtcttg ataaacgagc ttttcaatct cgtcgaggtt
     1861 aattcccggc ctcggcataa ctgaaaccca aaagagggat ggtccgcgac gttcgtccgg
     1921 tccggcggaa atactcagag ccagctcttt gtccttgaca aacttttgat aaagccgcga
     1981 ggataatcca ccggatagaa cctgccccaa cacctgcaag gcataagaat ccggagtatc
     2041 ccccggcgga attttataga cgatgtcgag tcggggcgtt tgagcaaagg gatcgtctat
     2101 ggttttacgg cgttcagcct gttgctccgg ctccgcgacg ttgggaacgg gtggcggcgg
     2161 ctgtgacgga atgttctcga agtatttttt aatcagcgcc attgcctctt cggtcttgaa
     2221 agcgcccacc aacgagagca ccgcattgtt aggcgcataa taggtccgaa agaactccgc
     2281 cacgtctttg acagtcgcgg catttaaatc ctccatggat ccgatggtcg agtgcttgta
     2341 agcgaagttg tcataggcgg tgccgatgac cgcttcatag gttcgaccat agggccggtt
     2401 gtcgactccc tggcgccgct cttcctggac ggtatgccgc tggttgtcca gattggcctg
     2461 attgatgttc aaagagcgca tgcgatcggc ttc
//
LOCUS       DASUTG010000865         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1914220, whole genome shotgun sequence.
ACCESSION   DASUTG010000865 DASUTG010000000
VERSION     DASUTG010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914220"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="VF089_20195"
     CDS             complement(<1..1648)
                     /locus_tag="VF089_20195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176337.1"
                     /translation="MPDWTQHVRPRLSGLRLSPTREAEIVEELSQHLDDRYRELIAGG
                     MSPEEAKRVALSDFREGNVLADYIAPLRQAHPPSAITPGASGSNLLSDLWQDIRYAVR
                     MLRKNPGFAAVVVITLALGIGANTAIFSFVNAVILNPLPFPDADRLVVISEISKEGME
                     IGVSLLNFRDFQVRAKSFEEIGGYRFDTFNLSGMGSPQRLIGQEVTGNYFRILGVQPQ
                     LGRTFTEEEEKFTPVGTAILISDSLWRTTFGSDPNILGRTLNLGGYDYWVIGVMPPRF
                     EFVRKTDLWAPLGGWLAPNSAWFHRENYMGLEAIAKLKPGISVSQGDIEMRQLATQMA
                     KENAETNSGASAYARSLQSMIVHEVRSTLLVLMGAVGFVLLIACVNVANLSIARALVR
                     EQEMGIRMALGAGRRRLIRQMLNESLLLSLLGGLAGILCARWLLSALIKLAPPNLARI
                     DDVQLNGRVLLFTAGITLLTGLMFGLLPAWSATRTKPAMVFSQRSSTSGPLRRRLFEG
                     LLVAEIALALMVVTGAGLMTRTMYKIAHVDPGFQSDHVLTM"
     gene            complement(1641..1994)
                     /locus_tag="VF089_20200"
     CDS             complement(1641..1994)
                     /locus_tag="VF089_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6176338.1"
                     /translation="MTAINTQALDRELKRGTAELLILSLLDARPRHGYELSKLIHTRS
                     GGQIQLHIDSLYPLLYRLEERGWIKGTWVEKPNERRRRFYKVTPNGRRVLTKQRETWA
                     AFVAAVQRVIGDEHA"
BASE COUNT          602 a          686 c          673 g          570 t
ORIGIN      
        1 gcatggtgag cacgtgatcc gattggaacc caggatcgac gtgggcaatc ttgtacatgg
       61 tgcgggtcat cagaccggca ccagttacca ccattagtgc aagagcgatc tctgccacta
      121 acaagccttc aaaaagacgg cgacgcaaag gcccagaggt tgacgatcgt tgactgaaca
      181 ccatggcggg tttagtgcgc gtcgcagacc atgcaggcag cagtccaaac atcaaaccag
      241 tcaacaaagt gattcctgct gtgaacagca acactcgtcc attcaattgc acatcgtcga
      301 tgcgcgcaag attcggtggg gccagcttaa tcaatgcaga caatagccag cgcgcacaaa
      361 ggattccggc caggcctccc agtagtgaca gcaaaagact ttcattcaac atctggcgaa
      421 tcaggcgacg acgtccagct cccagcgcca ttctgattcc catctcctgt tcccgaacga
      481 gcgcacgtgc aatcgacaga ttggcgacgt tgacacaggc gatgagcaag acaaatccaa
      541 cagcacccat caggaccagt aacgtcgagc gcacttcgtg aacgatcatc gattgaagtg
      601 aacgcgcgta tgcactggcc ccgctatttg tttccgcatt ctctttcgcc atctgagtcg
      661 cgagctgacg catctcaatg tcgccctgac tcactgaaat cccgggcttc agtttggcaa
      721 ttgcttcgag tcccatatag ttttcgcgat ggaaccaggc ggagttgggt gccaaccaac
      781 cgcccagtgg cgcccaaaga tcagtctttc taacgaattc aaaacgaggc ggcattacac
      841 cgattaccca atagtcgtag cctccgagat tcagggtccg ccccaaaata tttggatccg
      901 agccgaacgt agtccgccag agggaatcac tgatgagtat ggccgtcccc acaggtgtga
      961 acttctcctc ctcctccgtg aaagttcgac ccaactgtgg ttgaactccg agaatgcgga
     1021 agtagttccc cgtgacttct tgtccgatca aacgttgtgg actacccatg ccagataggt
     1081 tgaatgtatc gaatcgataa cccccgattt cctcgaacga ctttgcacgc acttggaagt
     1141 ctcgaaagtt aagcagcgaa acgccaatct ccatgccctc tttggagatc tcactgatca
     1201 ctacaagtcg atcggcgtct ggaaatggca gcggattgag aatgaccgcg ttgacgaagc
     1261 tgaagattgc ggtgttagcg ccgatgccta gagcaagcgt gattaccacc acagcggcaa
     1321 accctggatt cttgcgtaac attcgcaccg cgtatcgtat gtcttgccat aaatcgctga
     1381 gcaaattgct tcctgaagcc cccggtgtga tcgccgaagg cggatgggcc tgccggagcg
     1441 gcgctatgta atctgctaag acgttgccct ccctaaaatc ggaaagtgct actcgtttgg
     1501 cctcctcagg agacatgccg cccgcgatca actcccgata gcggtcatcg agatgttgcg
     1561 agagctcttc gacgatctcg gcctcgcgcg ttggcgagag gcgcaggccc gataaacgcg
     1621 ggcgcacgtg ttgggtccag tcaggcatgt tcgtctccga taacgcgttg aacggcggct
     1681 acaaatgctg cccaggtctc gcgttgttta gttaacacac gccggccgtt aggcgtgact
     1741 ttgtaaaatc ggcggcgtct ctcgttgggc ttttcaaccc acgtcccctt gatccaccca
     1801 cgttcctcga ggcgatacag caagggatag agggaatcga tgtgaagctg gatctggccg
     1861 ccggagcgcg tatggatcag tttactcagc tcatatccat ggcggggtcg cgcgtcgagc
     1921 agtgaaagga ttagcagctc tgcggttccc cgcttcagtt cacgatcgag cgcctgggtg
     1981 ttgatagctg tcatgaacag atagtatata ggcagacatt caagagtcaa tcactttaat
     2041 ttaggttttg tgcggtgact acggtgctct cgaaggagca attgttttca ctgtcttgac
     2101 aatcatcgat gtgcagcatg gtgcgccggc cgggcttaca acgaactgat ctaccgcggc
     2161 agcctcgtgg gaatagtttt cggaagcatt ggcggcggaa taattggtct aaccgttgcg
     2221 ctttgctgaa tgcaggcgcc gctagaggct tattggttgg aagcgtgatg ggcttgggac
     2281 tcaagattta tcacttgtaa gaatcggacc tctcgacacg gcacggacaa caagtagccg
     2341 gcaaacttcg tggtcttttg cattagtggt gcagcaaggg cagtgcagtt attgcgatct
     2401 aaacctaacc taagacacaa acacgatgtt tcgtggcaga gttactcgag gcttcattga
     2461 cgccattcgc gcaatcgata agaggtgccg ctctccaggt cctgccctat ataaggaagc
     2521 aatcttctgg c
//
LOCUS       DASUTG010000866         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1614652, whole genome shotgun sequence.
ACCESSION   DASUTG010000866 DASUTG010000000
VERSION     DASUTG010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614652"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF089_20205"
     CDS             <1..213
                     /locus_tag="VF089_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176339.1"
                     /translation="RNFPKHRDAVSIKGNSKKAADHKAQWTADPDRGWVTSEESHGIR
                     GGEQSWTAPKQMNGKSKGARKARKPF"
     gene            248..604
                     /locus_tag="VF089_20210"
     CDS             248..604
                     /locus_tag="VF089_20210"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6176340.1"
                     /translation="MDTRTKLLGRTKLLVLAGAMLLAGCAGGSLSTREKGAGVGALGG
                     AAAGGLIGAAVGHPGAGAAIGGAFGLGAGALVGDQLQGQEITNHQQQQQIQRNQAEIT
                     RNRQTIQRLERQRNEY"
     gene            complement(814..1233)
                     /locus_tag="VF089_20215"
     CDS             complement(814..1233)
                     /locus_tag="VF089_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6176341.1"
                     /translation="MLEMARVTPADVVYDLGSGDGGIIIRAAQKFGARGVGVEIDADL
                     VRKAQANASRHKVEHLTEFRLQDALSVDVSEATVVTLYMLPEFNAKLRPILDGQLRPG
                     ARVVSHDFPVEGWVPDRVERVNGDFFHDHTILLFEIR"
     gene            1517..2236
                     /locus_tag="VF089_20220"
     CDS             1517..2236
                     /locus_tag="VF089_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX6176342.1"
                     /translation="MASWDDVLTVRDKEVFSISGYGKRAGFGQRPAVLVIDVNYNFVG
                     DKPEPILESIKRFRNSCGAEGWEGVHRISELLAECRKKHLPTFYTTGHDDRTNSVAFG
                     RWQAKNSRSTEDMTDTWDKGNDIVQEIAPRAGDIIVRKQKPSAFFGTPLMSMLNEVHA
                     DSVLVAGTTTSGCVRASVIDAFSYNLKVSVVEECVFDRGQASHKINLFDMAMKYADVI
                     SLQETVDYIRGLPDNLFAPAL"
     gene            complement(2287..>2908)
                     /locus_tag="VF089_20225"
     CDS             complement(2287..>2908)
                     /locus_tag="VF089_20225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6176343.1"
                     /translation="IAILLIPNIVMDIAQVFRDGFPFAILRRFAWFFLATIAGVFLGT
                     AVLVNLPLWVLNFCLGTMVLIFVAFTWLKFEFRISPGLEKFLSMPVGFISGFLNGVTN
                     AAGPVLAIYLYSLRLEKRQFIKSIATIFVFTKMTQLAAVSSWNLFNASTLMLSLQVTL
                     FTLAGFYAGIKTQDRINQQTFNRALLILLSIIGVTLLARALTQSAP"
BASE COUNT          793 a          734 c          772 g          609 t
ORIGIN      
        1 agaaatttcc caaagcaccg tgacgctgtt tcaatcaagg gcaattctaa aaaagccgca
       61 gaccacaagg cgcagtggac ggcagaccca gaccgcggct gggtgacttc tgaagagagc
      121 catggcatca gaggaggaga gcaatcatgg accgcgccga agcaaatgaa cggcaaaagt
      181 aagggagccc ggaaggccag gaagcctttt taggggacgc accgatacgg aaaggaagag
      241 actgaaaatg gatacaagga caaaacttct gggaagaaca aaacttttag tgttggctgg
      301 agcaatgcta ctggccggct gtgccggtgg ctcgttgtcc acacgtgaaa agggggctgg
      361 ggtcggagca ctgggcggcg ccgcggcagg ggggcttatc ggtgccgctg ttggccatcc
      421 gggtgccgga gcagcaatcg gcggagcctt cggtctcggc gccggcgcgc tagtgggaga
      481 ccaactgcag ggtcaggaga tcaccaatca ccaacagcaa cagcaaattc aaagaaatca
      541 ggctgaaatc actcgtaacc ggcagacaat tcaacggctg gaacgccagc gaaatgaata
      601 ctagacctaa ggacgccgac agccacggcg gatgctctcc gatccgacgt ggcgtcttat
      661 aatcggttac ggaactgctt aagtttggcg gatagcgcgg cgcctgcaat ttacgccgtc
      721 cgcgattgct gttgaagctc cggagaatcc agcacgcctt tatgctagtt gtaaaggtta
      781 acttgcagct cgtacggttc ctaattcgga tcatcatcgg atctcaaata atagaattgt
      841 gtggtcgtga aaaaaatcgc cgttgacccg ctctacgcga tcgggcaccc aaccctccac
      901 tgggaagtca tgggagacga ctcttgcgcc gggtcttaat tggccgtcca gaatcggtct
      961 caacttggcg ttgaattccg gcaacatgta tagcgttaca acggttgctt cagacacatc
     1021 cactgacaac gcatcctgca gacgaaattc tgtgagatgc tcgaccttgt gtcgagatgc
     1081 attcgcttga gctttacgca caagatcagc atcgatttca acgccaactc ctcgggctcc
     1141 aaacttctgg gccgcacgaa tgataattcc accgtcaccg ctgccgagat cgtagaccac
     1201 atctgcggga gtcacccggg ccatctccaa catcctatca accacttcaa tcggcgtagg
     1261 tacaaacggt acttcgccat ccgtccacac agggatagcg caacccttta ggactaaaag
     1321 caagcaaaga aatgacaatt tctggagtga gcttctcatt gctactttaa cccgtcgtaa
     1381 acgacaacgc tgcttataag gtaaactttg ccgcagcgtc aattgctttc tcacgcatga
     1441 acgaacaggt cgttgacaaa ggctacagtt tgttgtgatc tggcacgtag aacatctgat
     1501 ccaaaggagg caagaaatgg caagttggga cgatgtgcta acggtacgag acaaagaagt
     1561 attctctatc tcgggctacg gtaaacgtgc cggcttcgga cagagacctg ccgtcttggt
     1621 gatcgatgtt aattacaact tcgtcggcga taaaccggag ccgatccttg agtcaattaa
     1681 acgtttccgg aacagctgcg gcgccgaagg ttgggaaggc gttcaccgga tcagcgagct
     1741 acttgccgag tgtagaaaga aacatctgcc gactttctac accaccggcc atgatgatcg
     1801 aaccaactcg gttgcctttg gccgttggca ggctaagaac agtcgttcca ccgaggatat
     1861 gaccgatact tgggacaagg gcaacgacat cgtccaggaa atcgctcctc gagcgggaga
     1921 catcattgtg cggaaacaaa aaccgagcgc cttcttcggc acgcccttga tgagcatgct
     1981 caatgaggtt cacgctgaca gcgtattagt cgccggcacc acaaccagcg gttgcgttcg
     2041 tgcgagcgtt atcgacgcat tctcttataa tctgaaagtt tcggtggtcg aagagtgcgt
     2101 gttcgatcgt ggccaggcat cgcacaagat caatcttttt gacatggcga tgaaatatgc
     2161 ggatgtcatc tcgcttcagg agacagtcga ctatattcgg ggattgcccg acaatctttt
     2221 tgctccagcg ctataaatcc ggttttgtga atcgaccaac tgcagccagc attctatcgg
     2281 cacctgtcat ggtgctgact gagttagcgc gcgcgcaaga agcgttacac cgataataga
     2341 cagcaggatc aagagagctc gattaaaggt ttgctgattg atgcgatctt gcgttttaat
     2401 acccgcataa aagccggcaa gagtgaataa cgtgacctgc agtgacaaca tcaaagttga
     2461 agcattaaaa aggttccagc tcgagaccgc agccaattgg gtcatcttcg tgaagacgaa
     2521 gatcgtcgct atcgatttga taaattgacg tttctccaat ctaagactgt agagataaat
     2581 cgccagcacc ggaccggcgg cattggtgac gccatttaga aaaccgctga tgaagccgac
     2641 cggcattgag aggaattttt ccaggccagg gctaattcta aattcaaact tcagccaggt
     2701 gaacgcgaca aatatcaaaa ccatggtacc cagacagaag ttcaataccc agagcggcag
     2761 gttcaccaaa accgctgtac cgagaaagac tccggcgatc gtcgcaagga aaaaccaagc
     2821 aaagcggcgc aatatggcga agggaaaccc gtcgcgaaat acttgagcaa tatccatgac
     2881 gatattggga atcagcagaa tggcaatg
//
LOCUS       DASUTG010000867         5091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1318240, whole genome shotgun sequence.
ACCESSION   DASUTG010000867 DASUTG010000000
VERSION     DASUTG010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318240"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            709..1002
                     /locus_tag="VF089_20230"
     CDS             709..1002
                     /locus_tag="VF089_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176344.1"
                     /translation="MKSVGFTLVILLLKGNAFAQQAKEQPQPGRYHVVTGKVAFMDSM
                     VLVDTWTGKTWKIGPGDTWTPMTRLDTPEQVKEWAQTHPKTSTATDKVKELAK"
     gene            complement(1281..1931)
                     /locus_tag="VF089_20235"
     CDS             complement(1281..1931)
                     /locus_tag="VF089_20235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6176345.1"
                     /translation="MVTVFLLRHGETAWNKLGRIMGRTQVPLGADGIQQIQKIAPLIA
                     TLDLNGIYTSPLRRAVQTTKLVATGTKLPICKSEGLNEIAFGEWTGQHFDELIDNELY
                     RRFIKSPATTLLPGGETISDVQRRGLKVIHQAAQKVPGGRFLFVSHGDVIRAILCHYM
                     KLPLNEYRRLRVDNGSLSALQTNGRWAEIKYVNYLHEIILASKEPYMAAGAAALRK"
     gene            1987..2145
                     /locus_tag="VF089_20240"
     CDS             1987..2145
                     /locus_tag="VF089_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176346.1"
                     /translation="MMETNSVPQTAELFVIDQFFERAVIPHIGHLGSRRYFKVLISIA
                     GQRQFCRD"
     gene            complement(2186..>5091)
                     /locus_tag="VF089_20245"
     CDS             complement(2186..>5091)
                     /locus_tag="VF089_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6176347.1"
                     /translation="VLSGNDRGPSGLWRHGISGDLPILLIRIDDAEDQNIVRQLLRAH
                     EYWRIKQLAFDLVILNEQAPSYVQILQEALETQVRASQSRLQHERQESFGGVFILRGD
                     LISAEERTLLQTAARAVLLSRRGTLAEQVERQQRSQAPALPLPRRLPATKSLDVLPAP
                     PDLEFFNGLGGFTADGREYVTTLGEGQWTPAPWINVIANPSFGFQVSESGSGYTWSLN
                     SRENQLTPWSNDPVSDPPGETFYVRDEDSGELWGPTVLPIREEDWPYTARHGQGYSRF
                     EHTSHGISLDLLQFVPLADPIKISRLAIENNSGRSRRLSVTAYAEWVLGVSRSGSAPF
                     IVTEIDQETGAMLARNAWNSEFAGRVAFADLSGRQTAWTADRTEFLGRNGTADHPASL
                     ERGDSLSGKVGAGLDPCAALQTRIELRAGGRAEIVFVLGETATTEDARTLIALYRGAD
                     LDGTLRAVTKHWDNVLGTVQVTTPNPAMDLMLNRWLLYQTLACRVWSRAGFYQAGGAY
                     GFRDQLQDVMALMVAQRDIARAQLLRAAARQFVEGDVQHWWHPPSGRGVRTRVSDDLL
                     WLPYTVIQYLELTDDFRLLDEVVPFLDGPTLEAGQEDLYFEPRVSAQTGTLFEHCARA
                     LDRSLSVGGHGLPLMGTGDWNDGMNRVGAGGKGESVWLGWFLHTTLWEFARLADARGE
                     HRRAETWRLHVSALKASLEREAWDGEWYRRAYFDNGTPLGSAGNAECRIDSIAQSWGI
                     ISGAAEPGRGARAMTAVEKHLVRRDDGLVLLFSPPFDHASPDPGYIKGYPPGIRENGG
                     QYTHAAIWSVIAFAALSDGDKAGELFSLLNPINHASTRAGVYRYKVEPYVVAGDIYAE
                     PPHVGRGGWTWYTGSAGWMYRAGIEWILGFRLRGASLYFDPCIPRAWRGFGIKFRYHS
                     SRYEIDIENPQGVTRGVSSVELDGISLGPGSMHIELADDGATHHVRVILGAKR"
BASE COUNT         1092 a         1484 c         1458 g         1057 t
ORIGIN      
        1 gtggagtcaa aggatcaaaa tcgtcatccc tttgtccagc tagtcaacag cgtacctgat
       61 agtcttctct cggggcggac atgcttgatg cgggcttgag caggaggatc tctcctccgg
      121 atgccgaaca agcccaagac agagtacgaa gttcgcgatg acttctctac aaagaccatc
      181 agccttcttc agtcctgggt agaggactgt tgcttgaatc ccgactgtcg atgtccgacg
      241 ataggacctg cccttgagga cgaacgcacc ctcgatattg gaacaccgca cacatcccgg
      301 cggctttgaa cgggcaccgc gtcgatgatg gaacgctgag tttggaggcg cgaagagggc
      361 tgcgccaaca tgcaccgaga aagggaaatc gttacgggga gctgtcgttg ccgcggtctg
      421 caagaaagac ggcatatcgc taacagaact ccgaagcggg agccgccgtg ggaaactgcc
      481 cgcgctcaca gtgaagatcg ttcagaacct agtgaaggat tatggcgtgc ccatcgccga
      541 cctcgcttgc caagtgggta tctcgacatc aggagtctca aagattctga cacggagttt
      601 gtccagctac tcaacagcgt ccctgcatgg tacgggaact atatcgactc acaagcaaaa
      661 atgttgcagg atgcaaccca agagaaggcg accatgaacg cgagatgtat gaaaagtgta
      721 ggtttcacac tggtgatatt gctgcttaaa ggcaacgcat tcgcccaaca agcaaaggag
      781 cagccccagc cggggcgtta ccatgttgtc acaggtaagg tagccttcat ggattcaatg
      841 gttctcgtgg acacatggac gggaaaaacc tggaaaatcg gccccggcga tacgtggacg
      901 cctatgactc ggctagatac gccggagcag gtcaaagagt gggcacagac tcatcctaaa
      961 acaagtacag ccacggacaa ggttaaagag ctggcaaagt agggaagcga gtagttttgg
     1021 cctggttttt tttcctattt tctcttcgag aagcaggcgc taggcttgcc tctgcgcgta
     1081 taggtcagct tgctgggtga ataggcttga tttctttgac gcataaaaat ccactagttc
     1141 ctttagattc ttttctcctt gcgaaataca tcgagatctc tcactgatct gaacctcttc
     1201 tgttcttaat ggccttctga gtagttccct cccgcgccag cacctggccc accgtacact
     1261 ctctattgaa ccagtgtcgg ttactttctc agtgccgcgg ctcccgctgc catataaggt
     1321 tctttacttg ccaggatgat ttcgtggaga tagttgacgt atttgatttc cgcccaacgg
     1381 ccgtttgttt gcagcgcgct caacgatccg ttatcgaccc gcaatcttct gtactcgttc
     1441 agcggcaatt tcatatagtg gcaaagaatc gcccggataa catcgccatg ggaaacgaaa
     1501 agaaatcgac cgccgggcac tttctgcgcg gcttgatgaa tcaccttcaa gccgcgccgc
     1561 tgcacgtcgg aaatcgtttc gccacccggc aggagggtcg ttgccggcga cttgatgaag
     1621 cggcgataca gctcgttatc gattagttcg tcgaaatgct ggcccgtcca ttcaccgaag
     1681 gcgatctcgt ttaatccctc gctcttacaa atcggcaact tcgttcccgt cgcaacgagc
     1741 ttagtggttt gcaccgcccg ccgtagcggg ctggtgtaga tgccattgag atccagcgtt
     1801 gcaattagcg gcgcgatttt ttgtatttgt tggattccat cggcgccaag cggcacttgg
     1861 gtccgtccca tgattcgtcc taacttgttc caggcggttt caccgtgacg tagtaggaag
     1921 actgttacca tttttcgtta gactccaaaa aagggccggc ttcgcgccgg cccttggtgt
     1981 agtgatatga tggaaactaa ctcagtgccg caaactgcag aactcttcgt aatcgatcaa
     2041 ttctttgagc gcgcggttat cccgcacatc gggcacttgg gatcgcggcg ctacttcaaa
     2101 gtattgattt ccatcgccgg gcagcgacaa ttctgccgag attaacgctt agcctgtttc
     2161 cgcgaggtct gatttcgccc agtgtctatc tcttcgcccc aaggataacg cgcacatggt
     2221 gcgttgcacc gtcatcggcg agctcgatat gcatgctgcc cggcccgagc gatatgccgt
     2281 cgagctcgac tgacgacacc cctcgagtaa ctccttgtgg gttttcgatg tcgatttcgt
     2341 agcgtgacga gtgatagcgg aacttgattc caaagccgcg ccaggcgcgc ggtatacaag
     2401 ggtcgaaata aagactggca ccgcgcaggc ggaaacccag gatccactca atgccagccc
     2461 gatacatcca ccccgctgag ccggtatacc acgtccaacc gccgcggccg acgtgcggcg
     2521 gttcggcata gatatctcca gcgacgacat agggctcgac tttgtaccgg taaacgccgg
     2581 cgcgggtgct cgcgtgattg atcgggttca agagagagaa aagttccccg gccttatctc
     2641 catcgctgag cgcggcgaaa gcgatgaccg accagatcgc tgcatgagta tattgcccgc
     2701 cattttcccg gatgcccggc gggtaccctt tgatgtaacc agggtccggg ctcgcatgat
     2761 cgaacggggg tgagaagagt aggacgagcc cgtcgtcgcg acgaacgagg tgtttctcaa
     2821 ccgccgtcat tgcgcgcgct ccacgacccg gttccgccgc accggagatg atcccccaag
     2881 attgggcgat cgaatcgatg cggcattcag cgttgccggc ggagccgagt ggcgtcccat
     2941 tgtcgaagta agcgcggcga taccactcgc catcccacgc ttctcgctcc agcgatgctt
     3001 tgagcgcgct cacatggagc cgccaggtct cggcgcgccg gtgctcgcca cgcgcatcgg
     3061 ccagccgggc aaattcccag agtgtggtgt gaagaaacca gccgagccag acgctctccc
     3121 cttttccccc agctccgacc cggttcatcc catcgttcca gtcccccgtg cccattagtg
     3181 gaaggccgtg accgccgacc gacaggctgc gatcgagagc ccgcgcgcag tgttcgaaca
     3241 aggtgccggt ttgcgccgac acgcgaggct cgaaatacaa atcttcctgc cccgcctcga
     3301 gagtgggacc gtcgagaaag gggacgacct cgtcgagcag tctgaaatcg tcggtgagct
     3361 caaggtactg aatgactgtg tacggcagcc ataagagatc atcggagaca cgggtgcgca
     3421 cgccgcgacc tgaaggagga tgccaccagt gctgcacgtc accctcaaca aactgccgcg
     3481 ccgcggcccg aagtaactgc gcccgagcaa tatctcgctg cgcgaccatt agcgccatca
     3541 cgtcttggag ctgatcgcga aagccgtacg cgccgcccgc ttgataaaac ccggctcgcg
     3601 accagacacg acaagccagc gtctggtaaa gcagccagcg gttgagcata aggtccattg
     3661 ccggattggg ggttgtcacc tgcaccgtgc caagaacatt atcccagtgc ttggtgactg
     3721 cacgcagggt cccatcaagg tctgccccgc ggtaaagcgc gatcaatgtc cgcgcgtctt
     3781 ccgtggtcgc cgtctcacca aggacaaaga cgatctcggc gcggccaccg gcacgcagct
     3841 caatcctcgt ttgcagggcg gcgcacggat cgaggcccgc gcctactttg cctgagagac
     3901 tgtcccctcg ttcgagggat gccggatggt cggcggtgcc gttgcgaccc aaaaattccg
     3961 tccgatcagc ggtccacgcg gtttggcgcc cgctcagatc ggcaaacgcc acgcggccgg
     4021 caaactcact gttccaagcg ttgcgcgcca gcattgctcc ggtttcctgg tcgatctcgg
     4081 tgacgatgaa aggagcggag ccgctccgcg aaacgccgag cacccactcg gcatatgcgg
     4141 tcacggaaag gcgtctggat cgacctgagt tattctcaat agcgaggcgg gagatcttga
     4201 tcgggtcggc caacggtacg aactgcaaga ggtcaaggga gatgccgtgt gacgtgtgct
     4261 cgaagcggct gtagccctgg ccgtgtcgtg cggtatacgg ccagtcctcc tcgcggatcg
     4321 gcagcaccgt cggtccccac agctcgccag agtcttcatc gcgtacatag aatgtctcgc
     4381 cgggcgggtc gctgaccggg tcattcgacc agggggtaag ttggttctca cggctgttga
     4441 gcgaccaggt gtaaccggag ccagactcag agacctgaaa gccgaaggac gggttggcga
     4501 tcacgttgat ccagggggcc ggtgtccact gcccctcgcc caaggtcgtc acatattctc
     4561 gcccgtcggc agtgaatcca cctaggccat tgaagaactc caggtccggt ggcgcaggca
     4621 aaacatccaa cgacttagtc gctggcagac gtcgtggcag cggtaacgcg ggcgcttggg
     4681 atcgttgctg acgttccact tgctcagcca gggtgcctcg gcggctcaag agcactgcgc
     4741 gggcggcggt ctggagtagc gtgcgttcct cggccgaaat gagatctcct ctaagaatga
     4801 acacgccgcc gaacgactcc tgtcgctcat gctggaggcg cgactggctg gcccgaacct
     4861 gggtctccaa agcctcctga aggatttgca cgtaggaagg ggcctgttcg ttcaagatca
     4921 caagatcgaa cgccaactgc tttatgcgcc agtattcgtg agcgcggagc aactgacgta
     4981 cgatattttg gtcttcagcg tcatcgatgc gaatgagcaa gataggcaga tcgccggaaa
     5041 tcccgtgtcg ccataatcct gaaggtcccc ggtcgttacc cgagagcacc g
//
LOCUS       DASUTG010000868         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1764892, whole genome shotgun sequence.
ACCESSION   DASUTG010000868 DASUTG010000000
VERSION     DASUTG010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764892"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF089_20250"
     CDS             <1..181
                     /locus_tag="VF089_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176348.1"
                     /translation="QGAAGSFVTDHEFTAATTRAGYPYMAGGVSIDERQLMAELGNNF
                     KPQADLCRGVGHLFG"
     gene            174..425
                     /locus_tag="VF089_20255"
     CDS             174..425
                     /locus_tag="VF089_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176349.1"
                     /translation="MADVGLVITNQKNEEVVNMSAGGPWFYIQLPPGKYDVKASFAGR
                     TKTISNIPVSNSQQISRMFHWDLPSEPEHPQLVKELVKN"
     gene            523..696
                     /locus_tag="VF089_20260"
     CDS             523..696
                     /locus_tag="VF089_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016914759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HEX6176350.1"
                     /translation="MAVTTTDPIKIIFAILLPPVGTFMEVGLTGHFWLNIVLTILGYI
                     PGIIHALYVILKH"
     gene            946..2337
                     /locus_tag="VF089_20265"
     CDS             946..2337
                     /locus_tag="VF089_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX6176351.1"
                     /translation="MKSRIVHFWNTINSSLWFIPAVMTFVTVAVSVVTISLDAMVKEK
                     LVDRLGWLWAGGPHGAREILSTIAGSMITVAGVVFSITVVVLALASSQFGPRLLRNFM
                     RDKGNQIVLGTFISTFTYCLLVLRTVRGEDGGSEFVPYVSVTFGIALALASLGVLIYF
                     IHHVSISIQAPIIIARVAAEFDRGVERMYPEMLGESADEQTEPTNVRDIPLELERDGG
                     RVPSCGTGYLEAVDSDSLMRIAREKDLLIRINFRPGEFIVEGSDLVLTWPRQIIDQSL
                     IDSINTAIILSSERTESQDILFSVHQLVEIAVRALSPGINDPFTAMTCVDHLGAALCR
                     LAQRKIPSPYRYDDEGKLRVIAKGAAFAEVADTAFTPIRQYGRSSALVTIRLLTTLGV
                     VAERVRREEDRQALVRHALMIERASRDALAEQAERAAVEDRYGSVLKLLGEDRSLRKT
                     VSSTACGAELGTG"
     gene            2349..2822
                     /locus_tag="VF089_20270"
     CDS             2349..2822
                     /locus_tag="VF089_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018747193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6176352.1"
                     /translation="MNNWPAQLAVLAEVFLAKLLGGIIGIERTTANKPAGFRTHMLVA
                     GAAALLVGLSDALIYRFNSGAHIDLIESDPIRVIQAIIAGISFLGAGTIFRHPKKQHV
                     EGLTTAASLLLSGAIGISVALRQFVLAVAVTVLTLAVLRGVDFIERRINNSIEKP"
     gene            complement(2947..>4262)
                     /locus_tag="VF089_20275"
     CDS             complement(2947..>4262)
                     /locus_tag="VF089_20275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX6176353.1"
                     /translation="QGTPKGPTRGQPSPSPDPFGGQEPFGGNDPFSEFWRRFFGDQFG
                     APGGPPAAPRRGLGSGFIIDQKGLVLTNNHVVENADKITVKLSDQRELEAKVVGRDPK
                     TDLAVIQISDGKGSFPVAPLGDSSGLQVGEWVVAMGSPFGLDNTLTAGVVSAKGRQIG
                     AGPYDNFIQTDASINPGNSGGPLVNLRGEVVGINTAIFSRTGGNLGIGFAIPINSAKE
                     ILPDLIKKGKVTRGWLGVSIQRVTPEIAQALGLEKNQGALVSSIVEGSPAAQAGVQAG
                     DVIVEFAGERIDDSSKLPAIVARTEVGKSVNMTVVRDKKRVPLSVKIAELKEEEVVAA
                     APQSGNLGMTVQNVTPDLAKSLGLNRADGVVITSVEPQSAAAEAGLQRGDVILEVDRS
                     KISNAAELRKTIEKAKPGANLLFLIQRGGNSVFLALKNPESKSPG"
BASE COUNT          883 a         1216 c         1206 g          957 t
ORIGIN      
        1 gcagggagcg gccggctcat tcgtcactga ccatgaattc accgcggcaa ctacccgagc
       61 cggttaccct tacatggccg ggggcgtcag tatcgatgag cggcagctaa tggcggaact
      121 cggtaataat ttcaaacctc aagctgacct ttgccgagga gtcgggcatc tatttggctg
      181 acgttggact ggtcatcacc aaccaaaaga atgaggaagt cgttaacatg tcagccggag
      241 ggccgtggtt ctacattcag ctgccgcctg gaaaatatga tgtgaaggct tcgtttgccg
      301 ggcggactaa aactatcagc aatattccgg tctccaactc ccagcagatc agccggatgt
      361 ttcattggga tttgccgtcc gaaccggagc acccgcagct ggtgaaggag ctggtgaaga
      421 actaggctcc ggcaagttcc agagtcagga acagaatcca gtgccgcggg cgctggagcg
      481 aaacccgggc agcctagcgg ctcccggcca aaggggttgt cgatggctgt gacaacgaca
      541 gatccgatca agatcatctt cgctatcctg ttaccgccgg tcggaacatt catggaagtc
      601 ggactgactg gccacttctg gttgaacatc gtgcttacga ttctgggcta tataccaggg
      661 atcatccatg ctctgtacgt aatcctcaaa cactgaactt tccccagcga ctacgcttcg
      721 cattgagcga agatacgcca tccaaccggc cccgccgagc gggcagcgat tgccgccgtt
      781 tgctgatccg cgccgctaat ctccgtccat accacagcac atgttgtgca tgacaaacac
      841 acggttcacc ggaggtgtat agaaaccggc cccgatcgtc actactggac ggagaatcct
      901 ggcgatgata ggactttttc gtggccaacc tatgaggggc agaaaatgaa atcccgtata
      961 gttcattttt ggaatacgat caattcgagc ctgtggttta tcccggcggt catgactttc
     1021 gtcacggttg cggtatcggt cgtgacgata agcttggatg cgatggtaaa ggagaagctg
     1081 gtcgatcggc tgggttggtt gtgggcgggc ggcccgcacg gggctcgtga aatcctatcc
     1141 acgattgcgg ggtcgatgat cacggtggca ggcgtggtct tctcgatcac ggtggtggtg
     1201 ctggcgttgg cgtcctctca gttcgggccg cgtctgctgc gtaacttcat gcgcgacaag
     1261 ggtaaccaga tcgtgctcgg cacgtttata tcaacgttta cctattgctt gctggtcttg
     1321 cgcacagttc gaggcgagga tggaggcagt gagttcgtgc cgtacgtctc ggtcacgttc
     1381 ggcattgcgt tggctttggc gagcttgggt gtgctcattt atttcattca ccatgtctcg
     1441 atatcgattc aggcgccgat tatcatcgcc agggtcgccg cagaattcga tcgcggtgtc
     1501 gagcgcatgt atcccgagat gctgggagag tctgccgacg aacagacgga acctacaaac
     1561 gtgcgcgata tcccgcttga gttggagcgt gatggaggtc gggtgccgag ctgcgggacc
     1621 ggctatttgg aggccgttga cagcgacagc ctgatgcgga ttgcaaggga aaaggatctg
     1681 ttaatccgta tcaattttcg acctggagag ttcattgtcg aagggagcga tttggttctg
     1741 acctggccga ggcagattat tgatcagagc ctcatcgaca gcatcaacac tgctatcatt
     1801 ctcagttccg aacgcaccga atctcaggat atactttttt ccgttcacca actcgtggag
     1861 attgctgtcc gggcgctgtc cccgggcata aacgatccgt ttaccgcgat gacttgcgtg
     1921 gaccatctcg gcgcagccct ctgccggctg gcgcagagaa agattccctc gccgtatcgt
     1981 tacgacgatg aaggaaagct gagagtgata gctaaagggg cggcatttgc cgaggtcgcc
     2041 gatactgcgt ttactccgat caggcagtac gggcgttcca gcgcgttggt cactattcgg
     2101 ctgctgacta cactgggcgt ggtagccgag cgcgtccgga gggaagagga ccgtcaggcg
     2161 ctcgtgcgcc acgcgctcat gatcgagcgc gccagccggg acgcgctagc agagcaggca
     2221 gagcgggcag cggtcgagga tcgctatggg agtgtcttaa agctccttgg agaggatcga
     2281 tcgttgcgga aaacggtcag ttcgacggct tgcggcgcag agctcggcac cggatgactc
     2341 agacttagat gaacaactgg ccggcccaac ttgcagttct agccgaagtc tttctagcta
     2401 agcttctcgg cggaattatt ggcatagaaa gaactacggc caacaagcct gccggatttc
     2461 gaactcacat gctagtggca ggagcggccg ccctactcgt agggttgagc gacgccctga
     2521 tttaccggtt caactcgggc gcccacatcg acttgataga atcggatccg atccgtgtca
     2581 tccaggcgat catagccggc attagctttc tcggcgccgg cactattttc agacacccga
     2641 agaaacaaca tgtcgaaggc ctaacgacag ccgcatcgtt gctgctttcc ggcgcaatcg
     2701 gaattagcgt ggcgcttcgc cagttcgtgc tagccgtcgc tgtcaccgtg ctgaccctcg
     2761 ccgtacttcg cggcgtggac ttcatagaac gccgaatcaa caattcaatc gagaagccat
     2821 gactctgcgt catcgtattt tcgaccggca aagtggtgca gtatggctca aacgattact
     2881 gcaccactcg atccggtaga cctgccgccg ctctgattct gccggaacca tctcgccgcc
     2941 atcggctcag ccagggcttt tgctctcggg atttttcaac gcgagaaaga cactgttgcc
     3001 gccgcgctgg atcaagaaga gcaggttcgc gccaggcttc gctttttcga tagtcttgcg
     3061 cagttccgcg gcgttgctaa ttttgctgcg atctacttcc aggatcacgt caccgcgttg
     3121 aagaccagcc tcagcggcgg cgctttgcgg ttcgacggag gtgatcacca caccgtcggc
     3181 gcggttcaat cccaggctct tggctaaatc gggcgtcacg ttttgcacgg tcatccccag
     3241 gtttcccgac tgcggcgccg cagcaaccac ttcctcctct tttaactccg cgatcttgac
     3301 gctgagcggc acgcgcttct tatctctcac gaccgtcata ttcacgcttt tacctacttc
     3361 ggtgcgagcg acgatggcgg gtagcttgct cgaatcgtcg atcctttctc cggcgaactc
     3421 gacgatcacg tcgcctgctt gcacgccggc ttgagccgcg ggacttccct cgacaatgct
     3481 cgagaccagc gccccctggt tcttctccaa tccaagagct tgagcgatct ccggagtgac
     3541 tctctgaatg ctcactccca accaacctcg agtgactttt cctttcttga tcaagtcagg
     3601 caggatctcc ttggccgagt tgattggaat ggcaaagcca atcccgagat tgcctccggt
     3661 gcggctaaat attgcagtgt tgatgccgac gacttcgccg cgcagattga ccagaggccc
     3721 gccagaattt cctggattga tcgaagcgtc ggtttgaatg aaattgtcat aagggccggc
     3781 gccaatctgc ctgcctttgg cactgaccac gcccgcggtc aaggtattat ccaagccgaa
     3841 cggactgccc atcgcaacca cccactcgcc cacttgaagc ccgctcgaat cgcctagagg
     3901 cgccactgga aaactaccct tcccatcgct gatctgtatc actgccaagt ccgtcttagg
     3961 atcccgcccg acgactttcg cttcgagctc tcgctgatcg gagagtttga cagtaatctt
     4021 atccgcattc tcgacgacat gattgttggt taagaccaat cccttctggt cgatgataaa
     4081 tccggaaccg agaccccggc gcggagcggc cggtgggcct ccaggcgcgc caaactgatc
     4141 gccgaaaaac cggcgccaga actcgctaaa aggatcgttt ccaccgaacg gctcctgccc
     4201 tccgaaagga tcaggggacg gagacggctg cccgcgagtt ggacccttgg gcgtcccctg
     4261 tg
//
LOCUS       DASUTG010000869         5629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1025527, whole genome shotgun sequence.
ACCESSION   DASUTG010000869 DASUTG010000000
VERSION     DASUTG010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025527"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF089_20280"
     CDS             <1..504
                     /locus_tag="VF089_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX6176354.1"
                     /translation="AVTAGHGTQELVERFEKDHDQYNSIMAKALADRLAEAFAEYLHK
                     RARAEWGYGKDEQLSTQELVAEKYRGIRPAPGYPAQPDHTEKPIIFDLLRVGDATGIA
                     LTESFAMYPAASVCGLYFAHPESHYFAVSSIGKDQVESYAARKGMKVSEVERWLASIL
                     GYEPGKR"
     gene            865..1338
                     /locus_tag="VF089_20285"
     CDS             865..1338
                     /locus_tag="VF089_20285"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX6176355.1"
                     /translation="MDKIFKKVVLLLVTGAGIGYIPRLPGTVGTIIAIPFSLALNRIA
                     LSNFLVATVLLIGSVAGAVWLCSQFAGMLNQKDPQLIVIDEIVGFLVGNFLAPFSLTA
                     LLWSFALFRFFDISKIFPANRLERLQGGAGIVLDDVMAGLYTFASLRLLLKLGWV"
     gene            complement(1652..1726)
                     /locus_tag="VF089_20290"
     tRNA            complement(1652..1726)
                     /locus_tag="VF089_20290"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1691..1693),aa:Glu,seq:ctc)
     gene            complement(1729..1804)
                     /locus_tag="VF089_20295"
     tRNA            complement(1729..1804)
                     /locus_tag="VF089_20295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1769..1771),aa:Gln,seq:ctg)
     gene            complement(1862..3286)
                     /gene="gltX"
                     /locus_tag="VF089_20300"
     CDS             complement(1862..3286)
                     /gene="gltX"
                     /locus_tag="VF089_20300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6176356.1"
                     /translation="MTQIRTRFAPSPTGYLHIGGARTALFNYLFARHYAGKFILRIED
                     TDRERSTSEAIEAILEAMNWLKLDYDEGPFFQTERLPLYKERIQQLVAAGKAYPCVCT
                     AQDLDAKRQLAQKEKRKPSYDGTCRPAEGVIPPLPEDKPYTIRFRSLQLGTTVVNDLV
                     KGHVIFENRELDDLIIARSDGTPTYNFCVVVDDIEMNITHIIRGDDHLANTPRQIQLY
                     HAMGQTLPQFAHVPLILGTDKARLSKRHGATSVTAYRDMGYLPEAVVNYLVRLGWSHG
                     DEEIFSREELIQKFSLESIGKSAGVFNPEKFLWLNSHYVKSRPLRQLATEVIPFIEAR
                     GYPVPKDLEWLEKMTATLKERAKTLVELVEQAHFYLSDDISIDEKAAKKFLLPEIKDP
                     LNSLIQKLSDLDDFSDRAVQRAFTEVLEQTGLSMGKLAQPVRVALTGSTTSPGIHEVI
                     AVLGKERTLKRLNTALRFLSSQNQ"
     gene            3416..5116
                     /locus_tag="VF089_20305"
     CDS             3416..5116
                     /locus_tag="VF089_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX6176357.1"
                     /translation="MVPTEAVPSNFIRNIVADDLKAGKHQNRVHTRFPPEPNGYLHIG
                     HAKSICLNFGLAAEFAGLCNLRFDDTNPSKEDVEYVESIREDVRWLGFDWGDREYYAS
                     DYFDRLYQFAVQIIKAGKAYVCDLNGEQMREYRGTLTEPGRNSPFRNRSIEENLDLFE
                     RMKAGEFPDGARTLRAKIDMASANLNLRDPVMYRILHASHHRTADRWCIYPTYDFAHG
                     QSDSIEGITHSICTLEFEDHRPLYDWFLDQLGIHHPQQIEFARLNLSHTVLSKRKLLQ
                     LVQQNHVAGWDDPRMPTLAGLRRRGYTAEAIRTFCERIGVAKRNSTVDIAMLEHCLRE
                     DLNRRAARAMAVLRPIKVVIENYPTGEVEHLEAVNNPEDLSSGTRLVPFSGILYIEQD
                     DFREEPPKGFFRLSPGREVRLRYAYIIKCIDVVKDPETGEIRELRCTYDPETKSGSSQ
                     SNRKVKATIHWVSADHAKEAEVRLYDHLFSKEDPDDVHDGVDWLTNVNPKSLEVLTDC
                     RVEPILAKARVGERYQFERLGYFCVDSVDSTERKPVFNRTATLRDSWANLQKATKRSK
                     "
     gene            complement(5179..>5629)
                     /locus_tag="VF089_20310"
     CDS             complement(5179..>5629)
                     /locus_tag="VF089_20310"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX6176358.1"
                     /translation="RWSEFKDFTATFSPSPTFLPLGFAISGFNFPEDWKNTSTLRFGS
                     YYAVNRNWELRGGLAVEESPIPNHTLNPAIPGADLLTLNAGVGYKWESFSVDLGYMAV
                     FYKTRRVTNNELEGLPATSPLGFAGAPGRDKYETFNNFVTVSLGYRF"
BASE COUNT         1383 a         1390 c         1513 g         1343 t
ORIGIN      
        1 gccgtaacag cgggccatgg aacccaggaa ctggtcgagc gctttgagaa ggatcatgat
       61 caatataact cgatcatggc caaggcgctt gctgatcgtt tggccgaagc gtttgcggaa
      121 tacctgcaca aacgagcgcg cgccgaatgg ggctatggca aagacgaaca gctgagcacc
      181 caggaattag ttgcagaaaa atatcggggc atccgtcctg cgcccgggta tccggcgcaa
      241 ccggatcaca ctgagaagcc gattatcttt gatctgctta gagtcggcga cgccactggc
      301 atcgcgctga ccgagagttt tgcgatgtat cctgccgcgt ccgtttgcgg cctctatttt
      361 gcccatccgg aatcacacta ttttgccgtc agctccatcg gtaaggatca ggtggaatcc
      421 tatgcagcgc gaaaagggat gaaagtctca gaagtggagc gttggcttgc gtccatttta
      481 gggtacgagc cgggaaaacg gtaagccagg cttgatctct taggcggccg aaccaaacat
      541 caggatggag acgctaaagt tcgcgccgtg tcgtatcggg atcgcggtta gtttttatca
      601 attgcaagac ggcgctgaaa agaggcgggg ttcgattatc atctcgtaaa gcccttaaac
      661 tttgtcgagc tcaagaataa gttgtctgag atccgttctg ctccgctctt ttatagcaat
      721 gccaaaattg ttgagatctc atcctagcga gaggccggat ccgaacttcc tcgactcacc
      781 ctatcatcaa tagccgtgct cagagttcca atccctcgag tcgactgtgc cgagatatct
      841 ggaagaaagt agttgccgcg cgcgatggat aaaatcttca aaaaggtcgt actgctactc
      901 gttaccggag cgggaatcgg ttatatccca cggctcccag gaacagtggg gacgattatt
      961 gccatcccgt tctcgctcgc gctaaatcgc atcgccctga gcaattttct cgttgcgaca
     1021 gtgttactga tcggatcagt cgccggtgcc gtatggctat gcagtcaatt cgccgggatg
     1081 ctgaaccaga aagatccaca gttgattgtc attgatgaaa tcgtcgggtt ccttgtgggc
     1141 aatttccttg ctcctttcag cctcaccgcg ttgctttgga gctttgcatt gttccgcttc
     1201 ttcgatatca gcaaaatctt cccagcaaac agattggaac ggctgcaggg cggcgccggc
     1261 attgtgctgg atgatgtgat ggcgggtctg tacacgttcg ccagcttgcg cctgctgttg
     1321 aaattgggtt gggtgtgatc tgagagacgg cttgcgctcg atttgacaac gattccattc
     1381 atcgacggca atcagtctcc cacgactgca cagtggcgat agttccgcca gcttcgaaga
     1441 gctgcaggag cgcccaggta tagactttcc attcgatggt agagaataaa aacttggccg
     1501 tggccttgtc gagagtgccc gtctcattca gaaaagacca caccacaaat cacaacacgg
     1561 aaggcggaag ataggtgtaa caccttttta ccggtgaact gatgcgagta ccgcgcgata
     1621 gagcctgtta tttggttacc ctggagaatg gtggtggtcc caacgggaat cgaacccgtg
     1681 ttcccgacgt gagaggccgg tgtcctaacc gctagacgat gggaccgttg gctgaggagg
     1741 aaggactcga accttcaatc gcctgatcca gagtcaggtg gcttaccaat tagccgactc
     1801 ctcaaggtca gagcgttcaa attaaaagaa tcaagcggtg aaatcaaggc ggattagagc
     1861 gtcattgatt ttgtgaagac aagaatcgca gcgcggtatt taatcgtttt aacgttcgtt
     1921 ctttgccgag aaccgcaatg acctcatgaa tgcctgggct agtggtcgag ccggtcaggg
     1981 cgacacgtac cggttgggcc aatttcccca tcgacaaacc ggtttgttcg aggacttccg
     2041 taaaggctcg ctgaacagcg cggtcgctaa aatcatcaag atcgctaagc ttttgtatca
     2101 ggctgttaag cggatccttg atttcaggga gcaagaactt ttttgcagcc ttttcatcga
     2161 tcgatatatc atccgagagg taaaaatgtg cttgttcgac aagctcgacc aaagtcttgg
     2221 ctcgttcctt gagtgttgca gtcatcttct caagccactc caaatctttg ggcaccgggt
     2281 agcccctcgc ctcgatgaac gggatgacct cagtggccag ctgtcgcagc ggacgcgact
     2341 tgacatagtg agaatttaac cagagaaatt tctcgggatt aaagacgccc gcagatttac
     2401 caatgctttc cagagaaaac ttctggataa gttcctcgcg agaaaaaatc tcttcatcgc
     2461 cgtgcgacca tccgagtcga accagatagt ttacgaccgc ctcaggaaga taacccatgt
     2521 cgcgataagc cgttaccgac gtcgcgccat gccgcttgct gaggcgcgct ttatccgtgc
     2581 cgagaattaa tggtacatgg gcaaactgcg gcaatgtttg tcccatagca tgataaagtt
     2641 gaatctgtcg tggcgtattg gcaagatgat cgtcccctcg gatgatgtgg gtgatgttca
     2701 tttcgatatc gtccacgacc acgcagaaat tgtacgttgg agttccgtca gaccgtgcaa
     2761 tgatcagatc gtcgagctcg cggttctcaa agatgacatg gcctttaacg agatcattga
     2821 caaccgtggt cccaagctga agcgagcgga atcggattgt ataaggcttg tcttccggca
     2881 acggcggaat gacaccttca gccgggcggc aagtcccatc ataagatggc ttgcgctttt
     2941 ccttttgcgc gagctgacgt ttggcatcga gatcctgagc tgtgcacacg cacggatagg
     3001 ctttgccggc cgccactaat tgttggattc tttctttata gagcggaagc cgctctgttt
     3061 gaaaaaatgg gccttcatcg tagtcaagct taagccaatt catagcttcg aggatagcct
     3121 cgatggcttc cgacgttgat cgctcgcgat cggtgtcttc gatgcggaga atgaatttgc
     3181 cggcgtaatg acgcgcgaaa aggtaattaa aaagagcggt gcgggcgccg ccaatatgca
     3241 gatagcctgt gggactaggc gcgaaacgag tgcggatttg agtcatcgcg gtcaacctaa
     3301 cagggagcca aaataggatc aacggcggcg cgtttaaaac agccttgatc gtgatagtat
     3361 agattaccaa ggggagaaat ctgtggatag taaattatca gctctaaaag atattatggt
     3421 acctacagag gcggtgccgt ctaattttat ccggaatatc gttgctgatg atcttaaggc
     3481 gggaaagcac cagaatcgtg ttcacacgcg atttccaccg gagcccaacg ggtatcttca
     3541 cattggtcat gcgaaatcca tttgtttgaa cttcggtctt gcggcagaat tcgctgggct
     3601 ttgcaatctg cgcttcgatg ataccaaccc aagcaaagaa gatgtcgaat acgtagagtc
     3661 catccgggaa gatgttcgct ggttgggctt cgactggggt gatcgagagt attacgcttc
     3721 ggattacttc gatcgcctgt atcagttcgc cgtgcagatt atcaaggccg gaaaggccta
     3781 tgtttgcgac ctgaacggtg aacaaatgcg cgagtaccgt ggcacgctta ctgaaccggg
     3841 ccggaatagc ccctttcgga accgctcaat tgaggagaac ttagacctgt ttgagcgcat
     3901 gaaggcgggt gagtttccgg acggcgcacg caccctccgg gccaagattg acatggcttc
     3961 ggcaaattta aatctgcggg atccagtgat gtaccgtatc ttacatgctt cgcatcatcg
     4021 gacggcggat cgatggtgta tctatccgac ttatgacttc gcccacgggc agtcagattc
     4081 aatcgaggga atcactcact ctatctgcac attggagttc gaggatcaca ggcctctata
     4141 cgactggttt ctcgatcagt tgggtattca tcatccgcag caaattgagt ttgcacggct
     4201 caatctgagc cacacagtat tgagcaagag aaagcttttg caactagtac agcagaacca
     4261 tgtcgctggc tgggatgatc cgcgaatgcc gaccttggcc ggtttgcgcc gccgcggtta
     4321 tacggccgaa gcgatccgga cgttttgcga gcgcattggc gtggccaagc ggaacagtac
     4381 ggtggatatc gctatgctcg aacactgcct gcgtgaagat ctgaacaggc gcgcagcgcg
     4441 agcgatggcg gtgttaagac ccatcaaggt cgtcattgag aattatccta ccggggaagt
     4501 tgaacactta gaggcggtca ataacccgga agatctgagt agcggcacgc ggcttgttcc
     4561 gttctcggga atactctaca tcgagcaaga cgacttccgt gaagaacccc ccaaaggatt
     4621 tttccggtta tcgcctgggc gtgaggttcg cctgcgctac gcatacatca tcaaatgcat
     4681 cgacgttgtc aaagacccag agaccggcga aatccgcgag ctgcgttgta cctacgaccc
     4741 agaaactaaa agtggttctt ctcagagcaa ccgaaaagta aaagccacca ttcactgggt
     4801 ctcagccgat catgctaagg aagctgaggt gcgcctctat gatcacctgt tcagtaagga
     4861 agatcccgat gatgtacacg atggcgtgga ctggttgaca aacgtcaatc cgaaatcgtt
     4921 ggaggtgcta acggattgtc gagtagagcc aattcttgcg aaggcgagag taggagagcg
     4981 ctatcagttc gaacgactgg gctatttttg cgtcgattcg gtggattcaa cagagcgcaa
     5041 accggtgttt aaccggacgg caaccttacg cgattcttgg gcgaaccttc agaaggcgac
     5101 aaaaagatcc aaatagcaac taggcacgtg tctgcgagtg atcagcgggt tttactacac
     5161 cttgaattgc gcctgatcct agaagcgata ccccaaagat acagtgacga agttattgaa
     5221 agtttcatat ttgtctctgc cgggggcgcc tgcaaagcct aatggactgg tcgcgggaag
     5281 gccctctaat tcattgttag tcactcttct ggttttgtaa aacaccgcca tatagccgag
     5341 atcaaccgaa aagctctccc acttatagcc tacaccggca ttcaaggtta acaaatcagc
     5401 accagggata gctgggttga gtgtgtgatt cggtatcggt gactcttcca ccgccagtcc
     5461 tcctcgcaac tcccagttcc tgttcacggc atagtaactg ccgaatctca gtgtgctggt
     5521 gttcttccaa tcctcaggaa aattaaaccc ggaaatagcg aagcccaaag gcaaaaacgt
     5581 agggcttggt gagaacgtcg cagtaaaatc tttgaactcg ctccaacgg
//
LOCUS       DASUTG010000870         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_875204, whole genome shotgun sequence.
ACCESSION   DASUTG010000870 DASUTG010000000
VERSION     DASUTG010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875204"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VF089_20315"
     CDS             complement(<1..926)
                     /locus_tag="VF089_20315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176359.1"
                     /translation="MILRYLLTLLVVAAWALVPRPAVAAPVRIAYSSISGAMLPLWVA
                     KERGFFDKHGVDVEVMYVRGVSVEALLAGEVQFIRASPPSVVRSTLKGADLVVIANTI
                     NVPVFSLMTTPEIERPEDLKGRKIGVTNLGDSPDLVLSLILEKWGLQRSKDVSVLAIR
                     GGMPELMVSVSKGFVDAGMISAPSNLRGLKLGLRELVDASTLGISYLNSPLSTRRSTI
                     KTQRDMVLRVLRGYHEGVQATRTDRDSTLKILSKYVRVDNPEILSEVYRIYGSKHLQA
                     DLSVQPEAVNGLLKGLGVEAAKANPASFVDSS"
     gene            complement(953..1699)
                     /locus_tag="VF089_20320"
     CDS             complement(953..1699)
                     /locus_tag="VF089_20320"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6176360.1"
                     /translation="MFKLEEKLSPDAFIETFFEVNRMDHQRAPHPWETLFRSGKCTRE
                     QLQGWAKERYYFTKQVPIKEYSILYNCPHTDVRRMWLPKAIEEEGEDLIGKPGKPHPE
                     YWLDVCEGLGMNREFVKNSEPLFGVKFAVDSFATAAFGASWLLGVAVSEGDDTARAMT
                     RDLDVFRTHYKWVPEEALEFYRLHAGVDVEHGAIRKEILRKYATTKELQEACINAQLM
                     KNNMRRVMADCIYMTYVVQGASWNAASVAA"
     gene            complement(1902..2270)
                     /locus_tag="VF089_20325"
     CDS             complement(1902..2270)
                     /locus_tag="VF089_20325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176361.1"
                     /translation="MNAPLKVSGIDHVVFHVKDLARARKFYVDFLGLEVDHERDWQCF
                     LKCGKQGIALFEVDEGEEVHGGSEVNHMALRLDSGEYERVKATLQDAGIEVHGRKGDP
                     HCIYFHDPDGHRLQLLMPDE"
     gene            complement(2303..>2634)
                     /locus_tag="VF089_20330"
     CDS             complement(2303..>2634)
                     /locus_tag="VF089_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176362.1"
                     /translation="GISLVRYVVTFSEKGAPVDYVRLGKMLSLGQYIALSSKAVRPNS
                     GKAFIDFFLGDESMRMLAKQGEFVNRKGIYPSLPDADKIQAVEMDDFDAQGFKEKTRE
                     YQKIFLK"
BASE COUNT          564 a          759 c          701 g          610 t
ORIGIN      
        1 aacgaactgt cgacaaaact ggccgggttg gcttttgcgg cttctactcc caggcccttc
       61 agtagcccgt tgacggcctc cggctggacg gaaagatctg cctgcagatg tttggatccg
      121 tagatccgat agacttccga gagaatctcc ggattgtcga cgcgcacgta cttcgacagg
      181 atctttagag tcgagtcacg gtcagtgcga gttgcctgca ccccctcgtg gtagccgcgc
      241 agaacacgaa gaaccatatc gcgctgcgtt ttgatcgtcg aacgccgggt gctcagcggc
      301 gagttgaggt aagatattcc cagagtcgag gcgtcgacca gttcgcgcaa tcccagtttc
      361 aagccacgca aattgctcgg cgccgatatc atcccggcat cgacgaaacc cttactgacc
      421 gataccatca gctccggcat gccgccgcgt attgccagca ccgatacatc cttgctcctc
      481 tgcaagcccc acttctccaa aatcaagctg agaacgagat ccggggagtc gcccagattg
      541 gtaacgccaa tttttcggcc cttgagatcc tccggtcttt cgatctcggg tgtcgtcatc
      601 agagagaaga ccggcacatt gattgtgtta gcgatgacca ccaggtcggc tcctttgagg
      661 gtcgacctga cgaccgacgg tggcgaggcg cggatgaatt gcacttctcc cgccaacaga
      721 gcctccaccg agacgccgcg cacgtacatg acttccacgt ccacgccgtg tttatcgaag
      781 aatcctcgtt cctttgcgac ccacagcggc agcatggcac cgctgatgga gctataagct
      841 atgcgcaccg gagccgccac ggcgggtctt gggacgagcg cccaagcggc gacaaccaga
      901 agcgtgagta gataacgtag aatcatcggg cgctttcagc gattatgtca aactaagcgg
      961 cgacactggc ggcgttccaa ctggcgccct ggaccacgta agtcatgtat atacaatccg
     1021 ccatgacccg tctcatgttg ttcttcatga gctgcgcgtt gatgcaagct tcctgcagct
     1081 ctttagttgt ggcgtatttg cgcagaatct cttttctgat ggcgccgtgt tcgacgtcga
     1141 ctccggcatg aagccggtaa aactccaggg cttcctccgg aacccactta taatgggtgc
     1201 ggaagacgtc gagatcccgg gtcatggcac gcgccgtgtc atcgccttcc gagacggcga
     1261 cgccgagcag ccaggaggct ccgaacgcgg cagtggcgaa gctgtcgacg gcgaacttga
     1321 ctccgaacag cggttcggag tttttcacga actcgcggtt cattcccaac ccctcgcaga
     1381 catccagcca gtattcggga tgaggcttgc ccggcttgcc aatcagatct tctccttctt
     1441 cttcgatcgc tttgggaagc cacatgcgcc gcacatcggt atgggggcag ttataaagaa
     1501 tcgagtattc cttgatcggc acctgcttgg tgaaatagta gcgctccttg gcccaaccct
     1561 ggagctgctc ccgggtacat ttgccgctgc ggaagagagt ttcccatggg tgcggcgcgc
     1621 gttgatggtc cattctgttg acctcgaaaa aggtttcaat gaacgcatcg gggctgagct
     1681 tttcttcgag cttgaacata aatttagact ccttgatcgg ataaaccgct atcgggaatg
     1741 aaatccgttg tcaacaaatg caaaataccc gggtgtgttc ctgcaacttt cgacagctga
     1801 aagcatcact taccattgga accgctgaaa cgcttggaac gtagcgaagc gatcgaccga
     1861 ttggaacggt tggaacggat cgatccgata agagcggccg gtcactcgtc gggcattaac
     1921 aactgcagcc gatggccatc gggatcgtgg aagtagatgc aatgcggatc gcccttgcgt
     1981 ccatggactt cgatgcctgc gtcctgcagt gtcgctttga cccgttcata ttcgcccgag
     2041 tcgagccgca gcgccatatg attgacctcg ctgccgccgt gcacttcttc gccctcatcg
     2101 acttcaaaga gtgcgatgcc ctgtttgccg catttgagga agcactgcca atcccgctcg
     2161 tggtcgactt ccagtccgag gaaatcgaca taaaatttcc tggcgcgcgc gagatctttg
     2221 acgtgaaaga ccacatgatc gataccgctt actttgagag gtgcgttcat gatcatcctc
     2281 cgtaaaggat ttagcccgct ttctatttca gaaaaatctt ctggtactcc cgggtttttt
     2341 ccttgaatcc ctgagcatcg aagtcatcca tttctacggc ttgaatcttg tcggcgtcgg
     2401 gcagggacgg gtagatgcct ttgcgattta caaattctcc ttgtttggcg agcatgcgca
     2461 tactttcatc gccgagaaaa aaatcgatga acgccttgcc gctgttaggt cgtaccgcct
     2521 tgctgctgag cgcgatgtat tgccccaggc tcagcatctt gccaagcctg acgtagtcga
     2581 ccggcgcgcc cttctccgaa aacgtcacga catatcttac caagctgatg ccta
//
LOCUS       DASUTG010000871         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_430819, whole genome shotgun sequence.
ACCESSION   DASUTG010000871 DASUTG010000000
VERSION     DASUTG010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430819"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VF089_20335"
     CDS             <1..444
                     /locus_tag="VF089_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176363.1"
                     /translation="AHCHLALGQIATDEQHIETALNHFTTALRFNPVKAKVGYVLHNN
                     VSYCLNQLGRYRDAEQHCRLAIEMDARRASAYRNLGISLEGQQNTVAAVWALVEAIKL
                     DSSDDRAREILKRLISEHPFLPIQCPWIEEGFAPSNADIQSGLAI"
     gene            754..1239
                     /locus_tag="VF089_20340"
     CDS             754..1239
                     /locus_tag="VF089_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6176364.1"
                     /translation="MIDQIDIKILSILQNSGRSRLADIADEVELSAPAVLERVKKLEV
                     NGVIKGYQALLDGKKVGKDITAFIGVSIGNQRDIDKFASQMLRNPDVLECHHITGDES
                     FILKVKSANTTSLEKLLGEIRSVEGVTRTVTRVVLSTAKESQMFDLSQGLAESQNPKK
                     K"
     gene            1443..>5391
                     /gene="gltB"
                     /locus_tag="VF089_20345"
     CDS             1443..>5391
                     /gene="gltB"
                     /locus_tag="VF089_20345"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006630922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX6176365.1"
                     /translation="MTLSGLPPKQGLYDPNFEHDACGVGFVVHIKGEKSHEIVEQALT
                     VLENLDHRGACGCEENTGDGAGILLQVPHEFLKRTCEGLGFQLPEAGDYGVGMVFLPD
                     NREQRQRFSKIIEEIVVAEGQRVLGWRKVPTDNFYLGETAKSYEPFVRQVFIGRGTGV
                     DDDMAFERKLYVIRRRAENEIRHANLAGADFFYIPSLSHRTIIYKGMLTPRQVGTFYP
                     DLSDPMLESAIAVVHSRFSTNTFPSWGRAHPYRYLIHNGEINTLRGNENWMHARQAML
                     ASPLFGDDLQKILPIIQEDGSDSAKFDNSLEFLALSGRSLPHAVMMMIPEPWENHDSM
                     DESKRAFYEYHSNLMEPWDGPASIAFTDGTVVGAVLDRNGLRPSRYYVTKDDLVIMAS
                     EVGVLEIAPENVAEKRRLQPGRMFLVDTGEGRIISDEEIKQQIAKSQPYGRWLRENVV
                     HFNELPDIPEKEPLANHGSTLRRLQAFGYNFEDLRINIAPMAQNGIQPVGSMGTDTPL
                     AVLSDRPQLLYNYFKQLFAQVTNPPIDPIREELITSTTLTLGLEGDLTDPRPESCRQL
                     RLSAPILRNSEMEKLRRIERPGIKSATLPIVFTANEGKHGLERALEDLFALADRAISD
                     GATILILSDKGVDQFHAPIPALLACSGLHHHLIRTGTRTRVGLVLESGEPREVHHFCL
                     LLGYGVQAINPYLVFECLNDMIHEGMLKGVGYHGAVKGYIKAAVKGVVKVMAKMGIST
                     IKSYCGAQIFEAVGLGQELVDKYFTWTPSRVGGIGIDEVAREAQRQHANAYRNYPLNG
                     HTLEVYGQYQYRKDGELHLFNPRTIHLLQKACRNNDYGTFKEYTQLIDDQSKRLATLR
                     GLMQLKYAAQPIPVEEVEPVHEIVKRFKTGAMSYGSIGKEAHEALAIAMNRLGGKSNT
                     GEGGEDPARYAPDANGDSRNSAIKQVASGRFGVTSYYLTQAREIQIKMAQGAKPGEGG
                     ELPGRKVYPWIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANHHARISVKL
                     VSEVGVGTIAAGVAKGHADVVLISGHDGGTGASPQTSIKHAGLPWELGLAETHQTLLL
                     NNLRSRITVETDGQLKTGRDVIVAAMLGAEEFGFATTALVTLGCIMMRVCHLDTCPVG
                     VATQNPELRAKFAGDPSHVVNFMRFIAQEMREHMAKLGFRTINEMVGRADRLEVRQAV
                     EHLKARGLDYGAILYQPKVGPDVGRYCSVPQNHGLENALDNQVLLDLAAPALERGEKV
                     KASLGIRNTNRVVGTILGSEITRRFGPEGLPEDTVHFHFEGSAGQSFGAFIPPG"
BASE COUNT         1338 a         1288 c         1479 g         1286 t
ORIGIN      
        1 gcgcattgtc atttggcgct ggggcagatt gcaacggacg agcaacatat cgaaaccgcg
       61 ttgaatcact ttaccacggc gctccgattc aatccggtca aggcaaaagt cgggtatgtc
      121 ttacataata acgtcagcta ctgcttgaac caactcggcc gatatagaga tgcggagcaa
      181 cactgccgac tggccattga gatggatgcg cggcgcgcta gcgcttatcg aaacctgggc
      241 atcagtcttg aaggacaaca aaatactgtt gcggcggttt gggcgttggt cgaagcgatc
      301 aaattggact caagcgacga ccgtgctcgg gagattctga aaagattgat ttcggagcac
      361 ccatttttgc ctattcagtg tccatggatc gaagagggct ttgccccgtc taatgccgat
      421 attcaaagtg ggttggcaat atagtgtaat ctataaatcg agaacatgct ttggggggtc
      481 tgctccctga agtttttgtc gtgatatccc cctgttctcc cacaggtgca taaacatttc
      541 ccctctgcac gttcctcagc tatctactta agtcatagag taacagcctc tcttctctgg
      601 ttcgcgtccg tcgcgtggta gcttttccgc caccgataca ttctctttgt gcgttcacga
      661 ttaaaatctt cttttgacgc gccttgacac atgctcgctc ctctgagata agattcttaa
      721 gtctggatcg attttatcct aagattttta gttgtgatcg accaaataga tataaaaata
      781 ctttctattc ttcaaaatag cggccgatct cggcttgctg atattgcgga cgaggtggag
      841 ctgtcagccc cggcggtttt agagcgtgta aaaaagctgg aagtgaacgg tgtcatcaaa
      901 ggctatcaag ccttgctaga tggaaaaaag gttggcaaag acatcactgc gtttatcggg
      961 gtatctatcg gaaatcagcg ggacatagac aaattcgcaa gtcagatgtt gcgcaatccc
     1021 gatgttttgg aatgtcacca tataacgggc gatgaaagct ttatccttaa agtgaagtca
     1081 gcgaatacga cttcactgga gaagttgctc ggcgagattc gctcggttga aggagtgact
     1141 cggaccgtaa cgagagtggt tttatcaacc gctaaagaaa gccaaatgtt cgaccttagt
     1201 caaggactag cagaatcgca gaacccaaag aagaagtgaa taaccgacac tgacttggtt
     1261 tacgggcttc ggccaataat tgttcagaat tgccggctgt gagttcacca aacgacgcga
     1321 aagataacag agacctcggt cgatctggtc gagtctcatt acgatcgttg cgcatgtcag
     1381 ttttcgatgg gcgttttagt gtttcatatc gaagttccag tctgtagagc aaaggtgaag
     1441 ccatgacatt atccggatta ccacccaagc agggacttta tgaccctaac ttcgagcatg
     1501 atgcttgcgg agttggcttt gtcgtacaca tcaaaggtga aaagtcccac gagattgtgg
     1561 aacaagcgct gacagttttg gagaacctcg atcatcgcgg cgcgtgcggt tgtgaggaaa
     1621 acaccggtga cggcgccggt atcttgctgc aagtaccgca tgaattcctg aagcgaactt
     1681 gcgagggctt gggcttccaa ctccctgagg cgggagacta tggcgttggc atggtgtttt
     1741 tgccggacaa tcgcgaacaa cgccagcggt tttcgaagat catcgaggaa atcgttgtcg
     1801 ccgaggggca gcgtgtcctc ggatggcgca aggtgccgac cgataatttc tatctcggag
     1861 aaaccgctaa atcctatgaa ccctttgtgc gacaggtttt tatcggacgt ggcactggag
     1921 tcgatgatga catggcgttt gagcgtaagc tgtacgtaat ccgtcgtcgt gcggaaaatg
     1981 aaatccgcca tgccaatctg gccggtgcgg attttttcta cattcctagt ttgtctcatc
     2041 gcacgatcat ctacaaaggc atgttgacgc cgcgccaggt gggaactttc tatccagacc
     2101 tgtctgatcc gatgctggaa tcagcaatcg ctgttgtgca ttcgcgtttt agcaccaaca
     2161 cgtttccgag ttggggtcgg gctcatccgt atcgctatct gatccacaat ggtgaaatta
     2221 atactctgcg gggcaatgaa aactggatgc acgcgcgcca agccatgttg gcgtcgccct
     2281 tgttcggcga tgatctgcaa aaaatacttc ccatcattca ggaggacggc agcgattcgg
     2341 ctaaattcga taattctctg gaatttttgg ctctgagcgg gcgctcgctg ccgcatgctg
     2401 tcatgatgat gatcccggaa ccgtgggaaa atcatgacag catggatgaa agcaaacggg
     2461 ctttctacga ataccacagc aatctgatgg agccatggga cgggcctgcg tcgattgcat
     2521 tcacggacgg gacggttgtc ggggcggtgc tcgaccggaa tgggcttcgt ccatccaggt
     2581 actatgtcac caaagacgat ctcgtgatca tggcttctga ggtcggcgtc ttggaaatag
     2641 cgcccgagaa tgtagcggaa aagcgtcgac ttcagcctgg acggatgttt ctcgttgaca
     2701 ccggtgaagg ccgcatcatc agcgacgaag agatcaaaca gcagatcgcc aagtctcagc
     2761 cgtatggtcg atggttaaga gaaaatgttg ttcatttcaa cgaattaccc gatatcccag
     2821 aaaaagagcc tttggcaaat catggcagta ccttacggcg tcttcaggcc tttggttaca
     2881 atttcgaaga cttgcggatt aatattgcac ccatggcaca gaacggtatt cagccggtgg
     2941 gctccatggg aacagacacg cccttggcag tcctgtcgga tagacctcaa ctgctctaca
     3001 actatttcaa gcaactcttt gcacaagtga ctaatccgcc catcgacccg atccgcgaag
     3061 agttgatcac ttcaaccacc ttgaccctag gattagaagg agatttgacc gatccaaggc
     3121 cggaaagttg ccggcaattg agattgtcag cgccaatttt gagaaattcg gagatggaga
     3181 agctgcgtcg gattgagcgg cccgggataa aatcggcgac gctgccgatt gtcttcacgg
     3241 cgaatgaagg caagcacggc ctggaacgcg ctttggaaga cttgtttgct ctagccgacc
     3301 gggcaatttc tgatggcgcg acgatactaa ttctctccga taaaggtgtg gatcaattcc
     3361 atgcaccaat tccggcactg ctcgcgtgct ctggactaca tcaccacctg atacgcacgg
     3421 gcacacgcac gcgtgttggc ctagtgctcg aatcaggaga gccacgggaa gtgcatcatt
     3481 tctgcttgct gctcggttat ggcgtgcaag cgatcaatcc ctaccttgtc tttgaatgtc
     3541 ttaacgacat gatccatgaa ggtatgctga agggtgtcgg ctaccatggt gcggttaagg
     3601 gatacattaa agccgcagtg aaaggcgtcg taaaagtcat ggccaaaatg ggcatctcga
     3661 ctatcaaatc ctattgcggc gcacagatct tcgaggccgt tggtttggga caggagctgg
     3721 tcgacaagta tttcacttgg acgccatcgc gcgtaggtgg tatcgggatc gatgaagttg
     3781 cgcgtgaagc gcagcggcaa cacgctaacg cgtatcgaaa ttatccattg aacggtcata
     3841 cattggaagt gtacgggcag tatcaatacc gcaaggatgg tgaattgcat ttgttcaatc
     3901 cacgcacaat tcacctgtta caaaaggctt gccggaacaa cgattacgga acgttcaagg
     3961 aatacacgca attaatcgat gaccaatcca agcgattagc cacgctgcgc ggcctcatgc
     4021 agttaaaata tgcagcccag ccgattccgg tcgaagaggt ggaacctgtt catgagatcg
     4081 tcaagcgttt caaaaccggg gcgatgtcgt acggatcgat cggcaaggaa gcgcatgagg
     4141 cgcttgctat tgccatgaac cgtctaggcg gcaaaagcaa caccggtgaa ggcggagaag
     4201 atccggcgcg gtacgctcct gatgctaatg gagattcgcg taatagcgcg atcaagcagg
     4261 tggcctccgg acgttttgga gtgacgagtt attatctgac tcaagcgcgt gagattcaga
     4321 tcaagatggc acaaggcgcc aagccggggg agggcggcga attgccgggc cgcaaggttt
     4381 atccatggat cgctaaagtg cgccattcga ccccaggtgt tggattgatc tcgccaccgc
     4441 cgcatcacga catttattcg attgaggatc ttgcgcagct aattcacgat ctcaagaacg
     4501 ccaatcatca tgcccggatc agcgttaaac ttgtttcaga ggtgggggtt ggcaccatcg
     4561 cggccggggt ggcaaaaggc catgcagacg tggtcttgat cagcggacat gatggcggta
     4621 cgggtgcttc gccccagacc agcatcaaac atgcagggtt accttgggag ctcgggctcg
     4681 cggaaactca tcagacattg ctgcttaata acttgcgcag ccgtatcacc gtggaaaccg
     4741 atggccaact aaagaccgga cgagacgtga tagtggctgc catgttgggt gctgaagagt
     4801 tcggtttcgc aacaacggcg ctggtaacct taggctgcat catgatgcgc gtatgccatt
     4861 tagatacgtg tccagtgggt gtggcaaccc agaacccgga attgcgtgca aaatttgccg
     4921 gagatccgtc acacgtcgtt aatttcatgc gcttcatcgc gcaagaaatg cgtgagcata
     4981 tggcgaagtt aggatttcgc actatcaatg agatggtcgg gcgagccgat cggttggaag
     5041 ttcgtcaagc tgtggaacat ctgaaagcaa gagggttgga ttatggcgcg atactatacc
     5101 aacccaaagt tggtcctgat gtcgggcggt attgtagcgt gccacagaat catgggttgg
     5161 aaaatgcttt ggataatcaa gtactgctcg atttggccgc gccggcttta gagcgcggcg
     5221 agaaggtcaa ggcgtctctg ggaatacgca acacgaatcg tgtggtaggc accatcctgg
     5281 gcagtgagat tacacgccga ttcggacccg aaggattgcc tgaagacacc gttcattttc
     5341 acttcgaagg ctctgcgggg caaagtttcg gagctttcat accacctggc g
//
LOCUS       DASUTG010000872         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2061570, whole genome shotgun sequence.
ACCESSION   DASUTG010000872 DASUTG010000000
VERSION     DASUTG010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061570"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF089_20350"
     CDS             <1..710
                     /locus_tag="VF089_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX6176366.1"
                     /translation="RKRRHGRAPKGILGHSFLGYLQNHDQVGNRAQGERSSHLLSVGR
                     LKIAAALVLTAPFIPMLFQGEEWAASSPFLYFTNHEDAELGRLVTEGRRREFAAFSAH
                     SDDVPDPQALDTFERSKLVWDECSRKPHAEMLDWHRQLIRLRHELAALSAGRLERVTV
                     TFDEQAKWLVMARGPIAIACNLSERRQQTPLSTGKDARIILASEKEVQLVGDRVELPP
                     DSVAIIEAVGIGSAMRQ"
     gene            713..>2157
                     /gene="malQ"
                     /locus_tag="VF089_20355"
     CDS             713..>2157
                     /gene="malQ"
                     /locus_tag="VF089_20355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX6176367.1"
                     /translation="MANVDNSEAIVKRYQDAFGNWREATDETCAAILATMGQTPEEGD
                     APVWVVRRGEQTHLRGPAELTLEDGGMLRVDSELPEDLPLGYHTLRYLDRGREIKLIV
                     SPGLCYLPEHLRIWGWSAQLYALRSAGSWGIGDFADLRELGRWSARELNAGLLLINPL
                     GAASPYAQQERSPYFPSSRRFRNPLYLRIEEIPGAAELQLDLEQLAIAGKALNLQQRI
                     DRDSVFKLKKDALDMLWQRFSGNSRFDNFCREQGEPLRQFAAFCALAEYYQAGWRQWP
                     GEYRTPNSPAVLRFAGERADRVRYFQWLQWLLDEQLAKAAVEISLMQDLPIGVDSNGA
                     DAWAWQEVFAEGFSVGSPPDEYNAQGQDWGLPPFIPSKLRAAGYDPFIQTVRGALRHA
                     GGLRIDHVMGLFRLFWIPQGGDPTAGAYVRYPEQELLAILALESERSKAYIVGEDLGT
                     VEEGVRTQLAQSRILSYRLLWFESEPPARYP"
BASE COUNT          439 a          600 c          656 g          462 t
ORIGIN      
        1 ttcgcaagcg cagacatgga agagctccca agggcattct cgggcactct tttctcggct
       61 acttgcagaa tcacgatcag gttggcaacc gcgcccaggg agaacggagc agtcatctgt
      121 tgagtgtggg ccgcctcaag attgctgcgg cgttggtact gaccgcacct ttcattccga
      181 tgttgttcca aggcgaagaa tgggccgcga gctcgccatt tttgtatttc acaaatcatg
      241 aggatgccga actgggtcgc ttggtaaccg aaggccgccg ccgggaattt gccgcgttca
      301 gcgcgcactc ggacgatgta ccggatcccc aagcgctcga caccttcgag cgttcgaagc
      361 tcgtttggga cgaatgcagc cgcaagccgc atgctgaaat gcttgattgg caccgccagc
      421 tgatccggct gcgccatgaa ttggccgcgc tctccgctgg acggctggaa cgagtcaccg
      481 tgaccttcga cgaacaggcg aaatggctcg tcatggcgcg tggtccgatt gcgatagctt
      541 gtaatctgag cgagcggcgg cagcaaacgc cgctaagcac cggcaaagat gcgcgcatca
      601 tcctcgcttc ggagaaagaa gtacaactcg ttggcgaccg agtcgaacta ccgccggact
      661 cggtggcgat cattgaagcg gtcggcatcg gttccgcgat gaggcaatga ccatggccaa
      721 cgtcgacaac tcagaagcta tcgtcaagcg ataccaggat gctttcggca attggcgtga
      781 agccaccgat gagacctgcg cggcaattct tgcaaccatg ggacaaacgc cggaagaagg
      841 ggatgcgccg gtctgggtcg ttcggcgtgg cgagcaaacg catctgcgcg gtccggcgga
      901 acttacgctg gaagacggcg gcatgttgcg agtggatagt gagctgccgg aagatctgcc
      961 gctcggttat cacaccttgc gctatctcga tcgtgggcgt gaaattaaat taatcgtcag
     1021 cccgggactc tgttatctgc ccgaacattt gcggatatgg ggttggtcgg cgcagctcta
     1081 tgcgcttcga tccgctggaa gctgggggat cggggatttt gccgatctgc gtgagttggg
     1141 ccgctggtcg gcacgagagc tcaatgccgg gttgcttttg atcaaccctc ttggcgcagc
     1201 atcgccatat gcgcagcagg agcggagtcc gtattttccc agtagccgcc gttttcgcaa
     1261 tccattgtac ctgcgaatcg aggagatccc gggcgccgct gaacttcagc tcgacctgga
     1321 acaactcgcg atcgccggca aagctcttaa tcttcagcaa cgcatcgatc gtgattcggt
     1381 tttcaagctc aaaaaagatg ccttagatat gctctggcag cgcttttcag gcaattcccg
     1441 cttcgacaac ttctgtcgcg agcaaggaga gccgctacga caatttgctg ccttttgcgc
     1501 gcttgccgag tactatcaag cgggctggcg ccagtggccg ggtgagtatc gcaccccgaa
     1561 ttcacctgca gtgctacgtt ttgccggtga acgggcggac cgcgtgcgct attttcaatg
     1621 gctgcagtgg ctgctcgatg agcagttggc aaaagccgcc gtcgagattt ccctgatgca
     1681 ggatctgccc atcggagtgg attccaacgg cgccgatgcc tgggcatggc aggaagtgtt
     1741 tgcggagggg tttagcgttg gcagcccgcc cgacgagtac aacgcgcaag gtcaggattg
     1801 gggtttgccg ccgttcattc cgtcaaagct tcgtgccgcc gggtatgatc ccttcatcca
     1861 gactgtacgg ggagcattgc gacatgccgg gggtcttcga attgatcatg taatgggact
     1921 attccgtttg ttctggattc cgcaaggcgg agatcccact gccggggctt acgtccgtta
     1981 tcccgaacaa gagctactag ccatactggc tttggaaagc gagcggtcca aagcgtacat
     2041 cgtcggcgaa gatctcggca cggtcgaaga aggcgtccgt acgcagctag cccaaagccg
     2101 catcctgtcc taccggctac tctggttcga gagcgagccg ccggcgcgat atcctga
//
LOCUS       DASUTG010000873         6006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_136485, whole genome shotgun sequence.
ACCESSION   DASUTG010000873 DASUTG010000000
VERSION     DASUTG010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136485"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..3411)
                     /locus_tag="VF089_20360"
     CDS             complement(442..3411)
                     /locus_tag="VF089_20360"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6176368.1"
                     /translation="MPRSSGVRSVRTHFLLIFFGTAIPLIILTAMAVFYVGRAEFRSA
                     KTGLKDTARALSGAVDRELQASIRTLEALATSKLIDQGNLRDLHQSVISILPSQPGWR
                     TVIMRDASGKDIFHSSFPFGTPRPETVQSASFDQVINTLRPTVMNYFVGPVTGPTIGV
                     RVPVLREGELKYVLTATIDAVRIDQILHEQRLDDGWYAAVFDRERVQIASSTANSEAN
                     RGRKPGPLISQFDDNADSAWISGPNRAGVDSYGAFVKAPLSGFYVGIIVPQSLMSRTL
                     LNSVWFISLIGVVAGAAGLFLITFYGRLVRHCTTYLIDLAHMIGKGEPVEIVAPAPVT
                     EVNLITSAMAEASVLLQTTKAERDKADAERDRFETSLQEARDNLSQRNAELRIVMDTM
                     AVGVARLDKNQKYLWVGKRLCEWLGKSPSEIVGHSIKEILEPELYALIEPRIRAVLSG
                     QLIEFETEWQMRLGRRWIHASYSPTYAADRAVNGWVEVITDIDKRKRAEEALDHLARF
                     PLEDPAPVLRANPEGVVMYFNPAAEKALAELPSANRDRLPTALAEAVSQALRDGHRRE
                     LEMYFGERLFSFLVVPILDRQYANLYGRDVTVQKAAEAALIEADRRKDEFLAMLGHEL
                     RNPLGIISSGVQLLRKLGPPDPNVIELREMIARQVTHTSRLLDDLLDVSRLSRGKIQL
                     VKGPCDLRDIVQKTAEDYVRELRENGLRLELTLSDEPLRTAADSTRLAQALNNLLQNA
                     CKFTEPGGTVWVSLDAENQTHAIIKVRDNGMGMDPEVLGWVFEPFRQVDESVDRSRGG
                     LGLGLALVKGIVELHGGEVAAASAGRRKGSEFTIRLPLDQNHAPSTAVVRVNTPEPVR
                     RRILVIEDNLAGAYSMRMVLENLGHTLETAHDGAAGINAAQRFRPDVVLCDIGLPVLD
                     GYSVARAIRQNPILDKCLLIAISGYARDEERAREAGFDAHLLKPVDFEKLEALLTVRT
                     GGSMMPKTKNGNASAES"
     gene            4001..4093
                     /locus_tag="VF089_20365"
                     /pseudo
     CDS             4001..4093
                     /locus_tag="VF089_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4318..5565)
                     /locus_tag="VF089_20370"
     CDS             complement(4318..5565)
                     /locus_tag="VF089_20370"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX6176369.1"
                     /translation="MSIVFELDLRPTVSAELFPALYQAYLKLKPGTRLRAILDKNPRQ
                     SYMGFVEAGIAHRIARLGDDEWEWTATRTAWDPMGNGPGVHHVGMSPDGQRLYAVDRE
                     RTVFMFDAALQRLTASVSVNKGTSHLAVHPRSGRVYVCERSTDSMVRLDAETLQTRGR
                     IATGDSPNLPAAAENGSVVILPGRNGTLTVAYDDEELRRVNLAIGGQPSASTVSHDGR
                     RAYVPDTARNLLVSVDLEQGSVLAETPVGDGPSHPIVTPDDYMVCVANSRSHDISLVD
                     THTFQLLATIPSGEAAHQTTLTAAGDVLLVANFFEDSVSIVDLPSRTRVAVVAVGPYP
                     HGLDIIPGDRYAVATHFGDSWVSIIDVAERKVVARVPAGLGSSHTTFSPESDRAYVAN
                     SLANTITVIDLNSLEATAQIGVA"
BASE COUNT         1347 a         1761 c         1562 g         1336 t
ORIGIN      
        1 acgaaggaat ccggcccgcg ctcctgctcc gccctcagca aagctctaaa ccaattagtc
       61 cgaccgctgg cttgcaagag catcgcggtg tgcacccggt cgagcgtcgt cgtttccaga
      121 ttcattcagt gggtaagtga agccctagcc gcaagatcga tggacgagac gaacactggt
      181 cggaggcgat tgcagttggc gattttacct tggttcatta gctgcagaac gaactcggat
      241 acaaagccgc gcatcgccaa gtcattaaaa gtagcggtac atatgagctc cgggagcaga
      301 ctgaaagtta tgggtccaat tttagcgctc aaaatgaggc gctagaaaac actagatttt
      361 ggaacgaaga ttctgagtct actgcaactt agcttggtcc taccccgaag ttctgacctc
      421 gcgaaatgag tgtactgaca ctcagctttc agcggatgca ttcccattct tggtctttgg
      481 catcatcgag ccgccagttc gcacagttaa cagagcttcg agtttctcaa agtccaccgg
      541 ctttaggaga tgcgcatcaa aaccggcttc gcgcgcacgc tcttcatccc gggcgtagcc
      601 gctgatcgcg atcaatagac acttgtccag tatcgggttt tgacgtattg cccttgcaac
      661 actataaccg tcaagtacgg gcagtccgat atcacaaagc acgacatcag gccgaaaccg
      721 ctgtgccgcg ttgattcccg ccgctccgtc atgcgccgtt tctagagtgt ggccaaggtt
      781 ttccagaacc atacgcatgc tatacgcacc cgctagattg tcctcgatca caagaatccg
      841 gcgtcgaacc ggctcgggcg tattgactcg taccaccgca gtcgacggag cgtggttctg
      901 gtcaagcgga agccgaatag taaactcgga gccctttcgc cggcccgcgc ttgctgccgc
      961 cacttcaccg ccatggagct ccacaatccc tttaaccagc gcaaggccga gtcctaatcc
     1021 gccgcggctc cgatccacac tctcgtcaac ctgtctgaaa ggctcaaata cccaaccgag
     1081 cacttccggg tccattccca tcccgttgtc ccgcactttt ataatagcgt gcgtctggtt
     1141 ttcagcgtcc aaactgaccc agaccgtccc acccggctct gtgaatttac aggcgttctg
     1201 caacagattg ttaagcgctt gtgcaaggcg ggtagaatcc gctgccgttc ttagcggctc
     1261 atcacttaga gtaagttcaa gtcttaagcc attctctctc aattcgcgaa cgtaatcctc
     1321 ggccgttttc tgtacgatat cgcgcaaatc gcatggcccc ttgaccaact gaatctttcc
     1381 gcgcgacaag cgtgatacat ctagcagatc atcgagcaga cgggaggtgt gcgtcacttg
     1441 tcgtgcgatc atctctcgca actcgataac attcggatct ggcggaccaa gcttacgcaa
     1501 cagctggacg ccactgctga tgatccccaa aggattccgc aactcgtgtc ccaacatcgc
     1561 cagaaattcg tccttgcgtc ggtcggcctc tatcaaagct gcttcggcag ccttttgaac
     1621 tgtcacgtcc ctgccgtata aattcgcgta ttgacggtcg aggataggaa ccacgaggaa
     1681 cgagaacagc cgttcgccaa agtacatttc cagttcgcgt cggtgcccat cgcgtagcgc
     1741 ctgagagact gcttccgcaa gggccgtagg tagtcggtcg cgattggcgc tcgggagttc
     1801 tgccaatgcc ttttcggcgg cagggttaaa gtacatcacc actccttcag ggttcgcacg
     1861 caggaccggc gccgggtctt ccaaagggaa ccgcgctaag tggtccaatg cctcttccgc
     1921 gcgctttcgc ttgtctatgt cggttataac ctccacccat ccgttcactg ctcgatctgc
     1981 cgcatacgtc ggagagtaac tggcgtgtat ccagcggcgg cctaatctca tttgccattc
     2041 agtctcaaac tcgattagct gaccggacaa cacagcacga atacgaggct cgatcagcgc
     2101 atagagttcg ggctccaaaa tctcttttat cgagtggccg acgatctcgg atggagactt
     2161 tccgagccat tcgcaaaggc gcttgccgac ccagagatat ttctggttct tgtcaaggcg
     2221 cgcaaccccc accgccatag tgtccataac tattctgagt tccgcgttac gctgggacag
     2281 attgtcccgt gcctcctgga ggcttgtttc gaatcgatcc cgctcggcgt cggctttgtc
     2341 gcgctcggcc ttggtcgttt gaagcagaac ggacgcttcc gccatagcac tggtaatgag
     2401 attgacctct gtcacaggcg ctggggcgac aatttccact ggttctccct tgcctatcat
     2461 gtgcgcaaga tcgataagat aagttgtgca gtggcgcacg agccgtccat agaaggtgat
     2521 caagaaaaga cccgctgcgc cagctaccac tccaatgagt gatataaacc agacggagtt
     2581 taggagagtt ctggacatca aactctgggg cacgattatt ccgacgtaga aacctgaaag
     2641 cggcgctttg acgaacgcgc cgtaagagtc cacacctgca cggtttggac cggatatcca
     2701 ggccgaatca gcgttatcgt cgaactgcga aatcagagga cctggtttcc ttccgcggtt
     2761 ggcttcactg ttcgctgtgc tgcttgcaat ttgtacacgc tctcgatcaa aaaccgctgc
     2821 ataccaacca tcgtctaatc gctgttcatg cagaatctgg tctattcgaa cggcatcgat
     2881 cgttgccgtg aggacatatt tcaactcgcc ttcccgaagc actggaacgc ggactccaat
     2941 tgtcggccca gtgaccggcc caacgaagta attcatcact gtaggccgga gagtatttat
     3001 aacctgatcg aaacttgcag actggaccgt ttcagggcgt ggcgtgccaa acgggaaaga
     3061 agaatggaaa atgtccttgc cggaggcatc tcgcatgatc actgttcgcc aacccggctg
     3121 gctcggcaaa atgctaatca cgctctgatg aagatcccta agatttcctt gatcgatcaa
     3181 tttggaggtc gcgagagctt ccagtgtcct gatgctagcc tgcagctcgc gatcgaccgc
     3241 acctgacaac gcccgcgctg tatccttcag gcccgttttt gcgctccgaa actcagcgcg
     3301 accgacgtaa aagacggcca tggccgtcag aatgatgagg ggaatcgctg tcccaaagaa
     3361 aattaatagg aagtgggtac gaacggaacg gacccccgac gacctgggca ttgaaactcc
     3421 cttggggcaa caaccactcg ccgcgcggtc gaaagagaac atcgcacctg cgatctttag
     3481 aagtgttaaa gaaaaatcca gcgtagcgga atgattttcg gctcctgtta gctatcgcgc
     3541 cgcgcccccg ggttgtcaag cacaatctga gacggtgaac agtaccggtc tttgtggtcg
     3601 aatattgtcg ccatctcccc cctttcggaa gctacgcggc aacgcctctg aatgctatcg
     3661 cgtcatagaa aattgacaat gatgactacc ttgtgacggt ttggatcagc atgggcttca
     3721 cgctctcgct acttcccgta aacgccggct tacccgcacg gcgcggcctg gatggctatg
     3781 gctgtcataa caacaccaaa agcagacggc tatcactgtc accgtggacc cttagctggt
     3841 gaggtctttg ccagcagggc ggagatgctt tgagcgctca agagaatcag cagcacggta
     3901 gaggccgcac ctgataagcg gcccgtcgag attaacgagt accttcaacg ccaaacacat
     3961 agcgaaccta actcccgtag atcgataatt ccttagcgtc gtctttcata tcgatttgaa
     4021 catggttcgc gcccgaatgg ttatgcatcc ctgtaattgg acacagagcg gttatcgaga
     4081 gattcagaaa cctctgaaag cactatgggg ttattgatct atgtaagccc tgctgactat
     4141 gaacgctcga tggctgttgc ttaatctact gtccgtgaaa tcggggtcag ctcagtctga
     4201 ccccggagtt ctgccgcaac tctttcgaga aaactaggcg gcacacctgt tctttcgctt
     4261 catcaggcca acctagcgtg gtaagtacct gacccatcac ggtccgggca aacgcgctta
     4321 ggccacgcct atctgcgcgg tcgcctcgag gctattcaag tcgataaccg ttatggtgtt
     4381 ggcgagacta ttggcgacat aggcacggtc ggattcgggc gaaaatgtgg tatgcgaaga
     4441 gccgagccca gccggcaccc gtgcaacgac tttgcgttcc gccacgtcga tgatgctgac
     4501 ccatgaatcg ccgaaatggg tggcaaccgc gtagcggtca ccgggaataa tgtcgagacc
     4561 gtgcggatac ggtcccaccg ccaccactgc gacgcgagtc ctgctcggta gatccacgat
     4621 gctcaccgag tcttcaaaaa aattcgctac cagcagaacg tctccagcag ccgtcaaggt
     4681 cgtctggtga gccgcttcac cgctcggaat cgtagccagc agttgaaacg tgtgcgtgtc
     4741 gactagtgag atgtcgtgcg atcgtgaatt agcgacgcag accatatagt catccggggt
     4801 gacaatgggg tgagacgggc catcgccgac cggcgtttca gccaacacgc tcccctgttc
     4861 cagatcaacg ctcaccagga gattgcgcgc cgtgtccgga acgtaggctc gccgcccatc
     4921 atgacttacg gtcgacgcgc tgggctgccc gccgatcgct aaattcaccc ggcgaagttc
     4981 ctcatcatcg taggccaccg tcagagtgcc attgcgtccg ggtaggataa cgacactgcc
     5041 gttttctgcc gcagccggta gattgggact gtcaccggtg gcgatccgtc cgcgcgtctg
     5101 gagggtttcc gcgtcaagcc ggaccatact atcagtgctg cgctcgcaga cgtagacgcg
     5161 acccgagcgc gggtgcactg ccaggtggga tgttcccttg ttcacggaca cgctcgccgt
     5221 caaacgctgg agggctgcat cgaacatgaa cacagtgcgt tcgcgatcca ccgcatacag
     5281 gcgctgcccg tcaggagaca tgccgacgtg gtgtactccg ggcccgttac ccatcgggtc
     5341 ccaagccgtg cgggtagcag tccactccca ctcatcatcc ccaagccgcg cgattcgatg
     5401 cgcaatgccc gcttcgacaa atcccatgta ggactgccgg ggatttttat cgaggatcgc
     5461 gcgcaaacgc gtccccggct tcagcttcag atacgcctgg tacagcgcgg gaaacagctc
     5521 cgccgatacc gtcgggcgaa gatctaactc aaatacaatg ctcatgctta ctttttttct
     5581 ttccttgctc aggtctccgc atagaactgg accgtacgtg gccagaaccg acattgtcaa
     5641 tctcttggtt tgttagaatc tgttgtcccg aagtaattgc gcagaagtca attgcggcga
     5701 aacccaacca catcaagatt cctggactcc gaatcgcgtc gagacctcgc gcgcttcgtt
     5761 ttttataccc ggcgccacac ctgcgcattc gaaaaacaag aatagaaagc ctgagaccca
     5821 acgcgcgggt cgccggagtt ctgccccggt cagtttcgca tctgctcgcc cgtcagcgcc
     5881 cactgtgtac gtttcttctc aggtaagttg gcgaacaggc aagtcgccgt ctcaaaatct
     5941 ttcagtttgc gccacacgtc acggggcaag cgcgggacaa acccgttgcg caccttactt
     6001 gcaaac
//
LOCUS       DASUTG010000874         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1616200, whole genome shotgun sequence.
ACCESSION   DASUTG010000874 DASUTG010000000
VERSION     DASUTG010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616200"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF089_20375"
     CDS             complement(<1..242)
                     /locus_tag="VF089_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176370.1"
                     /translation="MSYDTGLSMEERVTSLFQPDTLMPEQYLETFRRKLYLEPEKKLL
                     LAVLEDAIACFQKYLLARDGKGRVLFLEAEEWILDE"
     gene            complement(298..753)
                     /locus_tag="VF089_20380"
     CDS             complement(298..753)
                     /locus_tag="VF089_20380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6176371.1"
                     /translation="MSLMQIPRRVDYGLRAIIYLSTQDPEKCCSIAEIARQQNVPKKF
                     LEKIIQDLMRRGLIRSKRGSCGGYTLARMPDQISFYDVIEALEGPIAVNVCMNEEFSC
                     DQLPRCTMVGVWSEIQQKVTEVFTRTTLADLRRTPCRDNLSASSLSSAA"
     gene            complement(750..1982)
                     /gene="nifS"
                     /locus_tag="VF089_20385"
     CDS             complement(750..1982)
                     /gene="nifS"
                     /locus_tag="VF089_20385"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX6176372.1"
                     /translation="MTREIYFDNNATTRVLPEVFEAMAPYYAELYGNPSSIHRFGSQV
                     SEHLSQARAQVAGLIGAADPVEVIFTSCGTEGDNAAIRGLLEMRPEKRQIITTQVEHP
                     AILGLCQHLEKKGYRVTWLGVDQDGMLDLNELRDALTDETALVSIMFANNETGVIFPI
                     EQIGEIVKTKGVPFHVDAVQAAGKLPIDVKNSPIDLMTVSAHKFHGPKGVGALYVRRG
                     ITFPPFIIGGHQERGRRAGTENVAGIVGMGKAAELAARNQKDDSVRIRALRDQLQDSL
                     LESCPSARINGDKAKRLPNTLNVSFRYLEGESILVLLDQHGICASTGSACTAGSSEPS
                     HVLRAMRTPADWLQGAVRFSLSRFNTEEEVRFINEKMPSIVKRLEGLSALGKLAVQQR
                     NQTPGSDIPAAPEQGGRL"
     gene            complement(2008..3468)
                     /gene="sufB"
                     /locus_tag="VF089_20390"
     CDS             complement(2008..3468)
                     /gene="sufB"
                     /locus_tag="VF089_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6176373.1"
                     /translation="MKSANDTLEELAGREYKYGFITDIESDTVPPGLNEDIIQLISAK
                     KNEPEFMLEWRLKAYRYWAKLEKTQAEPKWAMVHYPSIDYQAISYYSAPKNKGDAPKS
                     LAEVDPELLNTYEKLGIPLREQERLAGVAVDAVFDSVSVATTFKGKLAEMGVIFCSFS
                     EALQNHPDLVQKYLGSVVPYTDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRIN
                     AAETGQFERTLIIAEERASVSYLEGCTAPMRDKNQLHAAVVELVAHDDAQIKYSTVQN
                     WYPGDAQGRGGIYNFVTKRGKCLGKRSKISWTQVETGSAITWKYPSCLLQGDDSVGEF
                     YSVALTNNYQQADTGTKMIHIGKNTKSTIVSKGISAGHGQNTYRGLVKILKGATGARN
                     YSQCDSLLLGDKCGAHTFPYLEVLNSTAQVEHEASTSKIGEDQLFYCRQRGISTEDAV
                     NLIVSGFCKQVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(3493..3822)
                     /locus_tag="VF089_20395"
     CDS             complement(3493..3822)
                     /locus_tag="VF089_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6176374.1"
                     /translation="MAVGISVTERAAARIRELVTAENREGQGLRVKVVGGGCSGLQYK
                     VDFDMPKGSDKIFEKDGARVLVDMKSLLYLNGTELDYKEELMQSGFVFQNPNVKKACG
                     CGASFTV"
     gene            complement(3853..5082)
                     /locus_tag="VF089_20400"
     CDS             complement(3853..5082)
                     /locus_tag="VF089_20400"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX6176375.1"
                     /translation="MELPIYMDNHATTQVDPRVIETMLPFFTQKFGNASSRNHVFGWD
                     SEAAVDRAREQVASLIHAASPQEIVFTSGATESDNIAIKGIADAYAQAGNHIVTCAIE
                     HKAVLDSCKSLERKGYRVTYLPVRATGLIALDKLRDALTDKTILISIMAANNEIGTIE
                     PVREIGQIAKEKGILFHTDATQAVGKIPLNVEEMGIDLLSLTAHKIYGPKGVGALYVR
                     SSKPRVKLSPLIEGGGQERGIRSGTLNVPGIVGLGKACEIAQKDMMADGERLTGLGQR
                     LKAQIVGQLDEVSVNGDPLQRLPGNLHLSFAYIEGESLLMALKDVAVSTGSACTSASL
                     EPSHVLRAIGLEEHLAHSSIRFGLGRFNTEEEVDYTARRVVEEVHRLRKLSPILRARK
                     ARLQSPAANHKSQESAS"
     gene            complement(5228..>6503)
                     /locus_tag="VF089_20405"
     CDS             complement(5228..>6503)
                     /locus_tag="VF089_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6176376.1"
                     /translation="IGLHQKDNAQLLELLQGLRSAGNSILIVEHDPETILSADYVIDM
                     GPGAGTEGGWVVATGSPDELMAKAHSLTGQYLSGNRKIPIPSSRRKGSGEFIVLKGAR
                     QNNLQDITVEIPVGLMTCVTGVSGSGKSTLVMDMLYREVSRRLRRLRIKSGALDDLIG
                     WQHFDRVIGVDQAPIGRTPRSNPATYAGIYDPIRDLFARLPESRVRGYRPNRFSFNTV
                     GGRCEACGGEGLMRIEMYFLADVFATCDICKGRRYNRETLDIKYKGHSIADVLELTVI
                     QGLDLFAHIPAIADRLHALVDVGLGYIRLGQAAQTLSGGESQRLKLAKELARRSSGRS
                     LYVLDEPTSGLHFADIEQLLELLHRLADAGNTLVVIEHNLEVIKNADYVVDLGPGAGK
                     SGGRVIARGTPEDVAAVSLSHTGRYLSKVLTT"
BASE COUNT         1488 a         1850 c         1583 g         1582 t
ORIGIN      
        1 tcttcgtcca agatccattc ttcggcttcg agaaatagaa ctctaccctt gccatccctg
       61 gctaacaagt acttctgaaa gcaagcaatg gcgtcctcca gaaccgcaag caatagtttc
      121 ttctcgggct cgagataaag cttccggcgg aacgtttcca gatactgttc tggcatgagg
      181 gtgtccggct gaaagagcga ggtcaccctt tcttccatgc ttagtcccgt atcatagctc
      241 ataactaaaa cctcccttta ggttacggct tgccttacgt cgaattttga gaactgatta
      301 cgccgcgctt gagagagaag aggcgctcaa gttatcccga catggagtac gccgtaaatc
      361 tgccaaagtt gttcgagtaa acacctccgt caccttctgt tggatttcac tccagactcc
      421 caccatagtg cagcgtggca gctgatcgca gctgaactcc tcattcatac atacattcac
      481 cgcaattggc ccttcaagcg cctcaatgac gtcatagaac gatatctgat ccggcattcg
      541 ggcgagcgta taccctccgc aggaacctcg tttggatctg atcaacccgc gccgcataag
      601 atcttggatg attttctcca aaaacttttt cggcacattt tgttgccggg caatctccgc
      661 aattgagcag catttctctg gatcctgagt cgagagatag atgatcgcgc gcaacccgta
      721 atccacacgt cgaggaatct gcatcaggct catagcctac ccccttgctc cggagccgct
      781 ggaatgtctg agcccggcgt ctgattccgt tgttggacgg ccaatttccc caatgcagaa
      841 agtccctcta atcttttgac gatggaaggc atcttttcgt tgataaaccg aacctcctct
      901 tctgtgttaa accggcttaa actaaaccga accgcgcctt gcaaccaatc ggctggcgtc
      961 ctcattgctc gcagaacatg cgacggctcg gaggagccgg cggtacaggc ggaacccgtt
     1021 gaggcgcaga tcccatgctg atcgagcagg actaaaatgg attctccttc caagtacctg
     1081 aaactgacgt tcaaggtgtt gggcagtcgc ttcgccttat cgccgttgat ccgcgcgctc
     1141 ggacaagatt ccagcaagga atcctgcagt tggtcacgca gagcgcggat ccttacagaa
     1201 tcatcctttt gatttctcgc agccaattct gctgccttcc ccatgccgac gatgccagcc
     1261 acgttctccg ttccggcacg ccggccgcgc tcctgatgtc caccaatgat gaacggtgga
     1321 aaggttatgc cgcgtcgaac atagagagca ccgacacctt tgggcccgtg aaatttatgc
     1381 gccgatacgg tcataagatc gatgggactg ttctttacat cgataggcag cttgcccgcc
     1441 gcttgcacgg catcgacgtg aaacgggact cccttggttt tgacaatttc gccgatttgc
     1501 tcaattggga agattacccc cgtttcattg ttggcaaaca taattgagac cagggccgtc
     1561 tcatcagtta aggcatcgcg cagctcgttc aaatccagca ttccgtcctg atccacacca
     1621 agccaggtga ctcggtagcc tttcttctcg aggtgctggc agagacccaa aatcgccgga
     1681 tgttccacct gagtagtaat gatctggcgc ttctccggcc gcatctcgag caagccgcgg
     1741 atcgccgcat tatctccttc ggtgccgcag ctggtgaaaa tcacttccac aggatcggcg
     1801 gcgccgatca aacctgcgac ttgagctcgg gcttggctaa ggtgctcgct aacctggctg
     1861 ccaaagcggt ggatgctcga cgggttgccg tagagctccg cataatacgg cgccatggcc
     1921 tcgaatacct caggcaagac ccgcgtagtc gcgttgttat cgaagtagat ctcccttgtc
     1981 attgttcact caccaagcgg atgactatca gccaacgctg ccttcaaggc tgaccccaag
     2041 aagcttctga gcttcaaccg caaactccat cggcaattcg cggaacacct gcttgcaaaa
     2101 tccactgacg atcagattga ccgcatcttc ggtagagatc ccgcgttgcc ggcagtaaaa
     2161 aagttgatct tcgccgattt tggaagtgga tgcttcgtgc tccacttgcg ccgtactgtt
     2221 caaaacttcc aagtagggaa aggtgtgcgc gccgcatttg tccccaagca gtagcgaatc
     2281 acattgagaa tagttccgcg caccggtcgc ccctttcaat attttaacta acccccggta
     2341 ggtattctgt ccatggcccg cagagatacc tttagatacg attgtgctct tagtgttttt
     2401 gccgatatgg atcattttgg tgccggtatc ggcttgctgg taattattgg tcaaggcaac
     2461 ggaatagaat tcccccaccg aatcatcacc ctgaagcaaa cagcttggat atttccaggt
     2521 aattgccgac ccggtttcca cttgagtcca tgaaatcttc gaacgctttc ctagacattt
     2581 gccacgtttt gtgacgaagt tataaatacc gccccggccc tgcgcatcgc ccggatacca
     2641 gttttgcacc gtcgaatact tgatctgggc atcgtcatga gccactaact cgaccacagc
     2701 tgcgtgcaat tgatttttat cgcgcatcgg cgcggtgcaa ccttcaagat agctgaccga
     2761 tgcgcgctcc tcggcgatga tcagggtacg ctcgaactgc ccggtctcgg cggcgttaat
     2821 ccggaagtac gtggacagct ccatcgggca gcgcacgccc ttcgggatat aacaaaacga
     2881 accatcgcta aacactgcgg aattcaaagc ggcaaaaaag ttgtcggtat agggaaccac
     2941 cgaccctaaa tacttctgaa ccaagtcggg gtggttctgc agggcctctg agaaggagca
     3001 gaagatcacc cccatctcgg ccaattttcc tttgaaagtg gtcgccacag aaacgctgtc
     3061 aaacaccgca tccactgcaa caccggcaag tcgctcttgc tctcgcaggg gaatgcctag
     3121 cttttcataa gtgttcaaaa gttcggggtc gacttccgcc aaactcttcg gagcatcgcc
     3181 tttatttttt ggcgccgaat aatagcttat cgcctggtag tctatggatg gataatgaac
     3241 cattgcccat ttgggctcgg cctgggtctt ctctaatttg gcccagtacc ggtatgcttt
     3301 caggcgccac tccaacatga attcgggctc attcttcttt gctgaaatca gctggatgat
     3361 atcttcgttt aaacctggtg gcacggtatc ggattcgatg tcggtaatga atccgtactt
     3421 gtactcgcgc ccggctagtt cctctagcgt atcgttagcg cttttcataa tgtaatttcc
     3481 tgatacttgc gttcagactg taaaagaggc gccgcaaccg caggccttct tgacgttcgg
     3541 attctgaaaa acaaatccgg attgcatcaa ttcttcttta tagtcgagct cagtgccatt
     3601 gagataaagc aggctcttca tatcgaccaa cacccttgct ccatctttct caaagatctt
     3661 gtcagagccc ttgggcatat cgaaatccac tttgtattga agacccgaac agccgccacc
     3721 gacgaccttg actcggaggc cttggccttc ccggttttcg gcagtgacca attctctaat
     3781 acgtgccgct gcacgttcgg tgacgctaat tcctacggcc atacatcgat ttctcctgtc
     3841 tagaccaaat gactaggagg cactctcctg tgatttgtga ttcgccgcgg ggctttgcaa
     3901 tctcgctttt ctggcgcgaa gaatcggcga tagcttccgc aaccggtgca cttcctcgac
     3961 gacccggcgc gccgtataat ctacttcttc ttcggtgtta aaccggccca atccaaaacg
     4021 aatcgaggaa tgggccaaat gctcctccaa gccaatcgcg cgcaaaacgt gtgagggttc
     4081 aaggctcgcc gaagtgcatg ccgaaccggt agaaaccgca acatccttga gcgccatcaa
     4141 cagcgactcg ccttcgatat acgcaaagct caggtgtagg ttcccaggca gtcgctgtaa
     4201 cggatcccca ttgacactca cctcgtctaa ttgaccgacg atttgggcct tcagtctttg
     4261 acccaatccg gtcagacgct caccgtcggc catcatatcc ttttgcgcaa tctcacaagc
     4321 cttgccaagc ccgacaatcc ccgggacatt gagagtaccc gaccggatac cccgttcctg
     4381 cccgccccct tcgatcagtg gactaagttt aacccgcggc ttggacgatc ggacgtacag
     4441 tgctcccacc cccttggggc cgtagatctt gtgcgcggta agcgaaagca ggtcgatgcc
     4501 catttcttct acattaagtg gaatcttgcc caccgcctga gtcgcatccg tatgaaacaa
     4561 gattcctttt tccttggcta tttggccaat ctcccttacc ggttcgatag tgccaatctc
     4621 attattggca gccattatgg agatgagaat cgttttatcg gtgagagcat cccgaagttt
     4681 atccagagct atcaaaccgg ttgctcgcac cggcaaataa gtcactcgat agcctttcct
     4741 ctccaggctc ttgcagctat ccagcaccgc tttgtgctcg attgcacagg tgacgatgtg
     4801 gttgcccgcc tgcgcgtacg catcggctat ccctttgatc gcgatattgt ccgactccgt
     4861 ggcgccgctg gtaaagacta tctcctgcgg cgatgccgca tggatcaaag aagcgacctg
     4921 ctcacgcgcg cgatctactg ccgcttcact gtcccatccg aaaacgtgat tacgactaga
     4981 agcgttgccg aacttctgtg tgaaaaacgg cagcatcgtt tctataaccc gcgggtccac
     5041 ctgtgtggtg gcgtgattgt ccatgtaaat aggcagttcc atagaaaaag gagacctgtc
     5101 gtcgacttat cccactagtc tggtctgatt taaagataac gaatcggata ctctcagtca
     5161 atgagtcgat cgacgaatta gctagatcac gaatctcaac aggaggcggc aaaaatcaga
     5221 gggattttta cgtggttagc accttactca agtagcggcc ggtgtgcgat agggataccg
     5281 ctgcgacatc ttctggtgtc ccacgcgcaa tgaccctacc gccgctcttc ccagctccgg
     5341 gcccaagatc gaccacgtag tcggcgtttt tgatgacttc cagattgtgt tcaatcacga
     5401 ccaaagtgtt acccgcgtcg gccaaacgat gcagtagttc taagagctgc tcgatatccg
     5461 caaaatgaag tccggaagtg ggttcatcca gaacgtagag agatcttccg cttgatcgtc
     5521 gcgccagctc cttggcaagc ttgagacgtt gcgactcgcc gcccgaaagc gtctgggctg
     5581 cctggccgag ccgaatatac cctaaaccga cgtcaactaa tgcatgaagc cggtcggcga
     5641 tagccggtat gtgggcgaaa agatccagac cctgaattac cgttagttct aaaacgtcgg
     5701 caatgctatg ccctttgtat ttgatatcca gggtttctcg gttatagcgg cggcctttgc
     5761 agatatcaca ggtagcaaag acatcggcca gaaaatacat ttcgatgcgc atcaaacctt
     5821 cgccgccgca agcttcgcat cggccaccaa cggtgttaaa tgagaaccgg ttaggcctat
     5881 aacctctcac acgtgactcc ggcaatcgtg cgaaaagatc acgaatcgga tcgtagattc
     5941 cggcgtacgt ggctggattg gagcgtggcg tcctgccgat tggagcttgg tcgacgccaa
     6001 tcacccgatc gaagtgctgc cacccgatga gatcatccaa ggcgccgctc ttgatgcgca
     6061 atcgccgcaa gcgtcgggaa acttcacgat aaagcatatc cattactaag gtgctcttcc
     6121 cggaacccga aactccggtg acacaagtca tcagtccgac cgggatttca accgtgatgt
     6181 cttgaaggtt attttggcgc gctcctttta aaacgatgaa ctcgcctgaa ccttttcggc
     6241 gcgatgatgg gatcggaatc tttcgattgc cggataaata ctgaccggta agcgaatgcg
     6301 ccttggccat gagttcgtcg ggtgagccgg tcgcgacgac ccagccgcct tcggttcccg
     6361 cgcccgggcc catatcgatg acgtaatcgg cgctgagaat agtttccgga tcatgctcga
     6421 cgatcaagat ggaattaccc gcgctacgaa gcccctgtag aagctccagc agttgcgcat
     6481 tgtctttttg atggaggcca atg
//
LOCUS       DASUTG010000875         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1766374, whole genome shotgun sequence.
ACCESSION   DASUTG010000875 DASUTG010000000
VERSION     DASUTG010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766374"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VF089_20410"
     CDS             complement(<1..784)
                     /locus_tag="VF089_20410"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX6176377.1"
                     /translation="MAVMFRKMVVAGVGLICGSLALDMRRHKIVGEIIGYGRSEASLR
                     LAKKRGIIDSYFLREDEMPEDVNFLMMGTPVQTIVPLTAAFLPRLKAGCVISDVGSVK
                     AEIVRGMDRLLPGNIPFVPGHPIAGSEQWGARAAKHGLFLGRRTILTPTRKTDKAALK
                     KVARIWHKVGAKVELMDPEVHDRVLGLISHLPHVLVYALVNALDRSRVRGVDLKSYCA
                     GGFKDFTRIASSRPELWRDICLMNRQALGQSLGDYINSLEQLR"
     gene            complement(838..1932)
                     /gene="hisC"
                     /locus_tag="VF089_20415"
     CDS             complement(838..1932)
                     /gene="hisC"
                     /locus_tag="VF089_20415"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6176378.1"
                     /translation="MKVTDQVPEYIRTLIPYAPGKPIEEVEREIGIFNSIKLASNENP
                     LGPSPLALDAMREKLAQLHLYPDGDCFYLKRGLTAKLNVTPEQLIFGNGSNEIIELAI
                     RTFMRPGDEAVMARQAFVVYKLIVQAVGGISQEVPLRNFTHDLEAIGAAISARTRIVF
                     LANPNNPTGTIFRKAAWENFLKQIRSDVLIIVDEAYFEYVEAADYPNSLDYHQQGKTL
                     LTLRTFSKLYGLAGLRIGYGIANEDVISLMHRVRQPFNVNAPAQWAALAALNDSEHVR
                     RSLDNNRKGLEYLTAEFARLGIQYVPSHANFILLRVGKGEDVFNRLLAQGIIVRPMAG
                     YQFPEYLRVTIGTMDENRKFIDGLKKAFEA"
BASE COUNT          482 a          611 c          506 g          490 t
ORIGIN      
        1 gcctcagctg ctccaaactg ttgatgtagt cgccgagcga ttggcccagg gcctgccgat
       61 tcatcaaaca gatatcgcgc cacaactctg gccggcttga agcgatacga gtgaaatctt
      121 tgaagccacc ggcgcaatag gacttcagat cgacaccacg cacccgcgaa cggtccagcg
      181 cattgaccag ggcatagacc aaaacgtggg gcaaatgact gatcaatccg agcacgcgat
      241 cgtgaacctc cggatccata agctcgacct tggcgccgac cttgtgccag atcctcgcga
      301 cttttttcaa cgccgcttta tcggttttcc tggttggcgt caaaatcgtc cggcggccca
      361 gaaacaaacc gtgctttgct gctcgcgcgc cccactgttc gcttccagct atcggatgac
      421 cgggaacaaa cggaatattt cccggcagca accgatccat gccccgaacg atctcggctt
      481 tgacgctacc gacatcgctg atcacacaac cggctttcaa cctgggcaga aatgccgccg
      541 ttagtgggac aatggtttgc accggtgtcc ccatcatcag aaaattcaca tcctccggca
      601 tctcgtcttc gcgtagaaaa tagctgtcaa taatcccccg cttcttagcc aaccgcagac
      661 tggcttcgct ccgcccgtag ccgatgatct cgccaactat tttgtgccgg cgcatgtcca
      721 gagccaacga accgcaaatc agtccgacgc ccgccaccac catcttgcga aacataacag
      781 ccatcaaact tccttacgcg gtgccgaggt gatccataag tcatcgcgga tcttacgtta
      841 cgcctcgaac gctttcttga gtccatcgat aaactttcgg ttttcatcca ttgttccgat
      901 agtcactctg aggtactctg gaaactgata tcccgccatt ggtcgcacga tgatgccttg
      961 agcaagcaag cgattgaaaa catcttcacc tttgccgacc cgcaacaaaa taaagttcgc
     1021 gtgactcgga acgtattgaa tacccagcct ggcaaactcc gccgtcagat actcaagccc
     1081 tttccggttg ttgtcgagac tgcggcgcac gtgctcgcta tcattcaagg ctgccaaggc
     1141 ggcccactgc gccggcgcat tgacgttaaa cggctggcgc acccgatgca tcaagctgat
     1201 aacatcctcg ttcgcaatgc cgtaaccgat tctcaacccg gcaagaccat aaagcttcga
     1261 gaaggtccgc aacgtcagca gggttttgcc ttgctgatga taatccaacg agttgggata
     1321 atcggcggct tcaacgtact cgaagtaggc ttcgtcgacg atgatcaaaa catcagaccg
     1381 aatctgcttt aaaaaattct cccaagccgc cttgcgaaaa atagttccgg tggggttgtt
     1441 cggattggcc agaaacacga ttcgggttct ggcactgatg gctgcgccga tcgcttccag
     1501 atcatgggtg aaattccgca gcggcacttc ttgcgagatt ccgcccaccg cctgcactat
     1561 cagtttataa acaacgaacg cctggcgcgc cataaccgct tcgtcgcctg ggcgcataaa
     1621 ggtgcggatt gccagctcga tgatctcgtt ggagccgtta ccgaagatca gctgctcggg
     1681 cgttacatta agttttgccg tcaagccgcg cttcaggtag aaacaatcgc cgtccgggta
     1741 gagatggagt tgcgccaatt tttcgcgcat ggcgtcaagc gccaacggcg agggccctag
     1801 cgggttctcg ttggatgcta gtttgatcga attgaaaata ccgatttcgc gctccacctc
     1861 ttcgatgggt tttcccggcg catacggaat caacgtgcga atatattccg ggacttgatc
     1921 tgtaactttc attttccgct cggataagag cccaggattt tgataaagtc acagctggtc
     1981 tcaagcgttt gtagcgctcg catgatcctc gcttcctttt gatgcccttt gaagtcgata
     2041 aagaacatgt attcccatgg cttgttttta atcggccgcg attcgattt
//
LOCUS       DASUTG010000876         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1916798, whole genome shotgun sequence.
ACCESSION   DASUTG010000876 DASUTG010000000
VERSION     DASUTG010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916798"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VF089_20420"
     CDS             <1..554
                     /locus_tag="VF089_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX6176379.1"
                     /translation="ILRAEPDHYFKPDQYFNPMNPQAHYETTGPEVYRQTDAKITHFV
                     AGIGTGGTIMGAGRYLKELNPDIQVIAVEPDDALHGLEGLKHMASSIVPGIYHEEQLD
                     RKIPVSTEDAYAMVYRLSQEEGILVGQSSGAAMFASLRIARKLHSGIIVTVFPDFGDK
                     YLSTSLWVGWRDRMAVGNLKFSI"
     gene            558..815
                     /locus_tag="VF089_20425"
     CDS             558..815
                     /locus_tag="VF089_20425"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX6176380.1"
                     /translation="MKKKREAVRERIYLTFPRTLVREPVLSSMAKQFDVGFNIRGSTV
                     TAEMGLMALEINGTRSEVDKAVAWLKEKGVTVESIEKNVVE"
     gene            complement(816..1277)
                     /locus_tag="VF089_20430"
     CDS             complement(816..1277)
                     /locus_tag="VF089_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176381.1"
                     /translation="MTLLRNLLKPSLLSSLLLLAVCAEGAENGSKPVQVKIGTILASN
                     ESDQFDARLKPMERQLKVMKYRSYRLLKEESQSVPWNDNATFEIPGGRSLAVSPQDSK
                     DKQIALKVRLIEGTKPLLDTTVRLQGRGYFLLGGPPHEGGALVISISASAQ"
     gene            complement(1234..1647)
                     /locus_tag="VF089_20435"
     CDS             complement(1234..1647)
                     /locus_tag="VF089_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176382.1"
                     /translation="MERLHSTMRTVLESNGSELQFDPFWQGLQQRIAQKSNWYDELGD
                     WFRRTCTAPRLAWAIPAVIIIVLAGLSIDSFFPSARIAIQPDNLATVESIDAYGRNVA
                     LLHENETNTTVIWLYQPQESENDSSTEPSETKPSF"
     gene            complement(1773..2327)
                     /locus_tag="VF089_20440"
     CDS             complement(1773..2327)
                     /locus_tag="VF089_20440"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6176383.1"
                     /translation="MELSDWQLVQKCQAGDIASFQELVSRYHQKVYMVIVGLLRNPDD
                     AMEVAQESFFRAYRKITSFQGQSSFYTWLYRIAVNICIDHQRRQKRNPLEFRESMDEV
                     FEQQNEVARDPFSDVHDRELREEIVSAIDDLSPEHKAVIVLRTLEGMSYKDIGEILGC
                     SEGTVMSRLHYARKKLQNKLSAFL"
BASE COUNT          653 a          683 c          641 g          632 t
ORIGIN      
        1 aaattttgcg agcggagccc gatcattact tcaaacccga tcaatatttt aatcccatga
       61 atccacaggc ccactacgaa accactggac cggaagttta tcgtcagact gacgccaaaa
      121 tcactcattt tgtcgcaggt atcggcaccg gtggaaccat aatgggcgct gggcgttatc
      181 ttaaagagtt gaatccggac atacaggtta ttgccgtgga acccgacgat gctctacatg
      241 gtctcgaggg gctcaagcac atggccagct caatcgtgcc cggtatttat catgaagagc
      301 agctggatcg aaagattccg gtctcgacgg aagacgccta tgcaatggtg taccgattga
      361 gtcaggaaga ggggatttta gtcggccaat catccggcgc ggccatgttt gccagtctga
      421 gaatagcgcg caagctgcat tcgggcatca tcgttaccgt ttttcctgat ttcggggaca
      481 agtacttatc tactagccta tgggtaggct ggcgcgaccg aatggcagtt ggcaacctca
      541 agttttcgat ctgaatgatg aagaaaaagc gagaagccgt ccgcgaaaga atctacctca
      601 cgttcccgag gaccctagtg cgagaacccg tgctgtcttc aatggcaaag caatttgacg
      661 tggggttcaa cattcgcggc tcaactgtaa ccgcggagat gggattaatg gccctcgaaa
      721 tcaacggcac acggagcgaa gtagacaagg ccgttgcatg gctcaaggaa aagggagtga
      781 cggtggagtc gatcgaaaaa aatgtcgtcg agtagttatt gcgcagaggc ggaaatcgaa
      841 atcaccaagg ctccgccctc atgcggcggc ccacctaata gaaaatagcc ccgtccttgg
      901 aggcgcactg tggtgtccaa caaaggcttg gtaccctcga tcaagcgaac cttcaaagcg
      961 atctgcttat ctttggagtc ttgaggcgag acggcaagcg acctgccccc gggaatttcg
     1021 aatgtcgcat tatcattcca cggtaccgat tggctctctt ctttaagaag tcgataagat
     1081 cgatacttca tcaccttcaa ttgcctttcc attggcttaa gccgcgcatc gaattgatcg
     1141 ctttcattcg acgctaggat ggtgccgatc tttacctgaa cgggcttcga accattctcg
     1201 gccccctcgg cgcacaccgc cagcagcaag agactagaaa gaaggcttgg tttcagaagg
     1261 ttccgtagaa gagtcattct cgctttcttg aggttgatac agccaaatca cggtggtgtt
     1321 cgtttcattc tcatgtagca gggcaacatt gcgtccgtag gcatcgatcg attcgaccgt
     1381 agccaaatta tcgggttgga tggcgattct tgcactgggg aaaaacgaat caatagacaa
     1441 tcctgcgagt acaataatga tcactgcagg aatggcccaa gcgagcctag gcgcggtgca
     1501 ggtgcgtcga aaccaatcgc cgagttcgtc ataccaattg ctcttctgcg cgatacgttg
     1561 ctgtaagcct tgccaaaacg gatcaaattg aagctccgac ccgttcgatt ccaagacggt
     1621 tcgcatcgtg gagtggagac gttccaatct gtgccgctct tcgcggcagg cggcgcaccc
     1681 ttcaacatgg agacgggtag cctcggcttg cccggcggca agttcgccat cgagccaggt
     1741 tcccagagtt tctctcgtcc tttcacattt actcataaaa aggcgctcag cttattctga
     1801 agtttttttc gggcataatg taagcgactc atgaccgttc cttcggagca gccgaggatt
     1861 tcgccgatgt ccttatatga catgccttcc aaagtcctca ggacgatgac ggctttgtgt
     1921 tcgggcgaca aatcatcgat tgcggacact atctcctctc tcagctcgcg atcgtgaaca
     1981 tctgaaaagg gatctctcgc aacctcattc tgctgctcaa agacctcatc catgctctcg
     2041 cgaaactcca gaggattcct cttttgacgc ctctggtgat cgatacaaat attgaccgcg
     2101 atccgataaa gccaagtgta gaaagaagat tgaccctgaa aactggtgat cttccgatag
     2161 gcgcgaaaga acgactcctg cgcgacctcc atagcatcat ccgggttgcg taataagcca
     2221 acgatgacca tgtaaacctt ctgatgatat cgcgaaacca gttcttgaaa gcttgcaata
     2281 tctccagcct gacatttctg gaccagctgc cagtcactta gctccacatt aaccctgcct
     2341 gtttgacgtt ttccgctatt ttttattcag ccgaaacaaa acgtgatatc aaaaccttga
     2401 agttttcgcg aattggctta aaatacccgt gatatctctc cattccgtcg atctactttt
     2461 catcaaggaa tcaattaatg ctggtcagat gcccaagctg cgcgaccact tacaaggtgg
     2521 ccgacgaact tgtaaccggt accactcccg cctttcgttg ttctcgctgc aaacacacgt
     2581 tcgaaggtga gctgaatgcg tcagtcgag
//
LOCUS       DASUTG010000877         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1917404, whole genome shotgun sequence.
ACCESSION   DASUTG010000877 DASUTG010000000
VERSION     DASUTG010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917404"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1250)
                     /locus_tag="VF089_20445"
     CDS             complement(330..1250)
                     /locus_tag="VF089_20445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6176384.1"
                     /translation="MAAERSAVRTAYNKLMSLIGLITPAFWQWFNHNAFQMAAALAYY
                     TLISLAPLIIILIAIVGPFFGDAATENYIVGAIAGIAGEESARAVQAIVHNAKQYGSG
                     VLASAVGVTLLLLGAGAVVGQLQQSLNVIFGVTPKADVDWWAFIRARFFSYAMLLAIG
                     FLLLVSLIVTTLLSAISKYFGDLLPYLTAIWPAIDLASSFALVALLFALIYKILPDVN
                     IAWKDALIGAALTSFLFSVGKFLIGLYLGRSTVAAAYGAAGSVVTILLWVYYSALIVF
                     FGAEITQLYASQIGSGVKANELADKTEPAK"
     gene            complement(1484..2680)
                     /locus_tag="VF089_20450"
     CDS             complement(1484..2680)
                     /locus_tag="VF089_20450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176385.1"
                     /translation="MRIFDSLRYRDYALFWSSDLLGSLGHFVQEVALYWIAYEITESA
                     MALGILGLCGAAPRLLLGALGGVLVDRYDRKRLLILIQFLSTIPVLIFLIVYSFGILR
                     FWHLLAVEILFGSIRAVNPSASQSILAELVPRQDLMNAVSLYTIGFNFARIIGPSIGG
                     ILILWIGVRGCYGIFLVSLLLSGCGLALIRLKDQRAANPEQDFVREFKQGFQYVWASP
                     VILSSIVAAYTFSIFIVTYQRFMPVFAKEIFEVGPDGLGLLMAAPGIGAIASLTFLAS
                     VGERWNRAWLLWITTTLTPIFLILFCLSPFFWLSVVLLGIVGAGQVSFRTISRVIIQM
                     ETPRDLLGRVMSVFNMDQGMRSVGSVVLGAFATLFGASLGLALTAGVSLVITTTLFYR
                     LLGRKA"
BASE COUNT          765 a          791 c          808 g          555 t
ORIGIN      
        1 aactgggaca agagccgcta tatcgatacc cagagttggg cgatcatgcc cggcgaggac
       61 aaggccgaaa ccggggtttc agaagcctgc gctaagcagc tgtcgatcaa aagctagccg
      121 gcgctaaaaa cttaggcgtc attgcactcg attccttcgc tacgacgtgg tgacgcctag
      181 cctgtctcgg gcgcaggcga agtatgactt acgcgcacat cggccgttaa cctcttttgg
      241 gaggctgacc ggccggttgt gctcgtgatc actggggatg gacgtggcct tgttcaggag
      301 tctgtccggg ttgccccctg ctacggggct tatttagccg gttcggtttt atcggctagc
      361 tcgttggctt tgacgccgct gccgatttga ctcgcataga gctgggttat ctcagctccg
      421 aaaaatacta tcagcgccga gtaataaacc catagcagga ttgtcaccac cgacccggcc
      481 gcgccgtagg ctgcggctac ggtgcttctt cccagataga ggccgattaa aaattttccc
      541 acgctaaaca ggaacgaagt caaagccgct ccgatcaggg catctttcca ggcaatattt
      601 acgtcgggca gaatcttgta gatcagggca aacaggagcg cgactagtgc gaacgacgag
      661 gcgaggtcaa tcgcgggcca gatcgcggtg aggtagggca gaagatcgcc gaaatacttg
      721 ctgattgccg ataaaagggt cgtgacaata agcgatacca gcagaagaaa accgatcgcc
      781 agaagcatcg cataggaaaa aaaccgggcg cgaataaaag cccaccagtc cacgtccgcc
      841 ttcggcgtga ctccgaagat gacgttaagc gattgttgaa gttgaccgac tacggcgccc
      901 gcgcccagca ataatagggt gactccgacg gcggacgcga gcacgccact gccatactgc
      961 ttcgcattgt gcacgattgc ctggactgcc ctggcgcttt cctcgcccgc gataccggcg
     1021 atcgccccga ctatgtagtt ctcggttgcc gcgtctccaa agaatggccc gacgatggca
     1081 attagaataa tgatcagcgg cgccaatgag ataagtgtgt agtacgcaag cgcagccgcc
     1141 atctggaaag cattatgatt gaaccactgc cagaaggcag gtgtgatcaa tccgattagc
     1201 gacatcagtt tgttataagc agtgcgcacg gcggaacgtt ctgcggccat cgtccattaa
     1261 agataaggga cctgcaatcc gattcgactg aaaaggcgca tccgtatgca cgagacagaa
     1321 aaaagttcag agtgagattt actccctcag ctgagcatgt cgggattaac tctgccccgg
     1381 ccatttactc tagcaatccg tgcaaagctg cgcgagcgta gcacctgggt gtgtacgatg
     1441 ctctgctgcg accttctcat tgagttctcg gcgaagttcc tgatcacgct ttccgcccca
     1501 gcaatcggta aaaaagtgtg gtggtgatga ccaatgaaac gccagcggtg agcgcaagcc
     1561 ctagagacgc gccgaacagt gttgcgaagg cgcccagaac caccgaaccg accgaacgca
     1621 tcccctggtc catattgaaa acgctcatca cccgtcccaa gagatcccgc ggtgtctcca
     1681 tctgaataat tacccggctg atggtgcgga agctaacctg tcctgctccg acaatgccaa
     1741 gtaggacaac cgaaagccag aaaaacgggg agaggcaaaa aagaatgaga aagatcggtg
     1801 tcagagtcgt agtaatccac aagagccatg cccggttcca acgctcgccc actgacgcga
     1861 gaaaagtcag cgatgcgatt gcgccgatcc caggcgctgc cataagcaag cccaatccgt
     1921 ccggcccaac ctcaaagatc tccttggcga agaccggcat gaagcgctga taggtgacga
     1981 tgaaaatgct aaaagtgtag gccgcgacga tgctgctcaa gatcacgggt gaagcccaaa
     2041 cgtactgaaa gccttgcttg aattcgcgca cgaagtcttg ctcgggatta gcagcccttt
     2101 ggtctttcaa tcggatgagc gccagtccac atccggaaag aaggaggctt accaaaaaaa
     2161 taccatagca gccgcggacg ccgatccaca gaatcagaat gcctcctatg gagggaccga
     2221 tgatgcgcgc gaagttaaaa ccgattgtgt agagggaaac cgcattcatc agatcctggc
     2281 gcggaaccaa ctcggcaagg atagactggg aggccgaggg atttacggcg cggatcgaac
     2341 caaacagaat ttccaccgcg agcaagtgcc agaaccgaag aatcccgaag ctatagacga
     2401 ttaagaagat caacaccgga atggttgaga ggaattggat gagaatcaac aggcgcttgc
     2461 ggtcgtagcg gtccaccagc acgcccccga gcgcgcccag gagcaaacgc ggcgcggcgc
     2521 cgcacagacc caatatgccc aaggccattg ccgactcggt gatctcgtaa gcgatccaat
     2581 agagcgccac ttcttggacg aaatggccga gagaacccag cagatcgctg gaccaaaata
     2641 gcgcgtagtc tctgtagcgc agcgaatcga agatgcgaat ggggtggtct ccttaccaaa
     2701 cgctctttat agcgtgtccg ccgtttataa gaaaggaatt ggcctctaac atatctcgat
     2761 caaagagact ttcccaaaag tcggccctaa gcgcccgctc gtatcttctg gtgcggaatc
     2821 gaagttgacg tgttaaacag aggcgctatc gacctcgtgt gagcagagct agtcaggatg
     2881 actacgttcg aacaaaaacg gtgatcctgt ggaaaggcc
//
LOCUS       DASUTG010000878         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1917738, whole genome shotgun sequence.
ACCESSION   DASUTG010000878 DASUTG010000000
VERSION     DASUTG010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917738"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF089_20455"
     CDS             complement(<1..600)
                     /locus_tag="VF089_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176386.1"
                     /translation="MIPSKQTKWTDISTYQQWVETEGVPIIREFFIQDIKKVPLEPWD
                     RMGGLGVYLNLIGTGEANDAYIYEIPPSQKSKEQHHLFEEMIYVISGHGATSIWQTNG
                     KKQTFEWQTGSLFSPPINCHYQHFNGSGSEPARMLAVTNAPVLLNLFHNADFVFNNSY
                     VFSDRFSGEEEYFSGKGNSFPGRIWDTNFVPDVHTMALQS"
     gene            675..821
                     /locus_tag="VF089_20460"
     CDS             675..821
                     /locus_tag="VF089_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176387.1"
                     /translation="MQETALSTSKNQGGIRPICDGTRWLLEKGDLQAREMPHIGLLIV
                     LAKD"
     gene            complement(906..1775)
                     /locus_tag="VF089_20465"
     CDS             complement(906..1775)
                     /locus_tag="VF089_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase"
                     /protein_id="HEX6176388.1"
                     /translation="MRRGEFGAAWSISDILLRRHLAAPRAAIDVPRHQQVIWDGASLQ
                     CKRVLVRCYHGLGDTIQFVRYVPLLKEIAAQVAVWSQPSLIALLSTMNGIDRMIPLHD
                     GTPDCDYEADVEVMELPYVFRTTIETIPARVPYFQVDASALDYCKMKVGLVWRGGDWD
                     PRRDVPFELLTQLTDLQEISFYVLQQEASALEHHEKFKMILPPGADALTTARVMRALD
                     LVVSIDSMPAHLAGALGVRTWTLLQKDADWRWMIDRNDSPWYPTMRLFRQQQAGDWQP
                     VVAQVKRKLRELL"
     gene            complement(2072..>2320)
                     /locus_tag="VF089_20470"
     CDS             complement(2072..>2320)
                     /locus_tag="VF089_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176389.1"
                     /translation="GKGLVPPINPSAGHARLSANNPIPTFFQTGAAREPYNRGQWGSA
                     LKATPQATMSDVIDGRGLQTTSPFTCPRFPASLASLSD"
BASE COUNT          542 a          620 c          632 g          526 t
ORIGIN      
        1 agactgaagc gccatggtgt gaacgtcggg aacaaaattc gtatcccaga tccttcctgg
       61 gaacgaatta cctttgccgg aaaaatattc ttcctcgccg gagaaacgat ctgaaaacac
      121 ataactgttg ttgaagacaa aatcggcgtt gtgaaataga ttcaacagca caggcgcgtt
      181 ggttacggcc agcatacgcg ccggttccga accgctgccg ttgaagtgct ggtagtggca
      241 attgattggc ggtgaaaaca aactcccggt ttgccactcg aacgtttgtt ttttgccatt
      301 ggtctgccaa atcgatgtcg cgccgtggcc gctgattaca tagatcattt cctcaaaaag
      361 atggtgttgc tccttgcttt tttggctcgg aggaatttcg tagatgtagg cgtcgttggc
      421 ttcgccggtg ccgatgagat tcaagtaaac gcccagcccg cccattcggt cccatggctc
      481 aagcggaact tttttaatgt cctgaatgaa aaattcgcga atgatcggaa cgccctcggt
      541 ttcgacccac tgctgatagg tagatatatc cgtccatttg gtttgtttgc tgggaatcat
      601 cactccctcc gtctcataac tctcgtgaat cgattctatg aattagaatc gggtatatcc
      661 acgacgattg ctttgtgcaa gagaccgcgc tctcgacatc caaaaaccag ggtgggattc
      721 gtccaatctg cgacggcact cgatggttgc ttgaaaaagg agatctgcaa gcgcgcgaga
      781 tgccgcatat cggtttgctg attgtgctgg ctaaggatta gaccggaaaa cggcattagc
      841 agctcaggaa gggagaggag ctcatgggac tactgcgcca ggcaggagaa gtgaagttta
      901 cgagatcaga gaagttctcg cagcttgcgt ttcacttgcg ccaccaccgg ctgccaatct
      961 cctgcttgct gctgtcgaaa cagacgcatg gtcgggtacc acgggctatc gttgcggtcg
     1021 atcatccagc gccaatccgc gtctttttga agcaatgtcc aggttcgaac acctaatgca
     1081 ccggctaaat gagccggcat gctatcgatc gaaacgacca gatcgagcgc ccgcataacg
     1141 cgcgccgtcg tcaaagcgtc ggcgcccggc ggcaaaatca ttttgaattt ttcatgatgt
     1201 tccaatgcgc tcgcctcttg ttggagcacg tagaagctaa tctcctgaag gtcggtgagc
     1261 tgagtaagca actcgaacgg cacatcccgg cggggatccc aatcgccgcc tctccagaca
     1321 agccctactt tcatcttaca atagtcgagg gcagacgcat cgacctggaa atagggaact
     1381 cgagccggta tggtttcgat ggtcgtacga aacacgtacg gcaattccat cacttcaacg
     1441 tcggcctcat agtcgcaatc cggagttccg tcgtggagcg gaatcatgcg gtcaatcccg
     1501 ttcatcgtgc ttagcagcgc tatcaacgac ggctgcgacc aaaccgctac ctgcgcggca
     1561 atctctttta agagcggaac atagcggacg aactgaatcg tgtcgcccag accatggtag
     1621 caccgcacca gaacgcgttt gcactgcagc gatgcgccgt cccagataac ctgttgatga
     1681 cgtggcacat cgatagcggc gcgtggcgct gccaagtggc gccgcagcaa tatgtcgctg
     1741 atgctccagg cggcgccgaa ctcgccgcgc cgcatgtgct ccatccactg gacggtcgag
     1801 tcgatcgagt ggtttggatt gcgagtcacg tagttgattg aaacgcgaat ttcaaacgca
     1861 gacaagccgg aggactaagg caagactctc atcggatcta atccgcatgt acctgccgga
     1921 attcggacgc atctgcgcca ttgccgggca gacatttgca ggcgggattt agaactcgtg
     1981 attgttactc gcgcaactgc ggagaactcg cgatcaaagc ttgtccaatg tccgggcgca
     2041 actaacccta atcccacaat aattcacgtc actaatcgga cagcgaggct agtgaggctg
     2101 gaaaccgtgg gcacgtgaac ggactggttg tctgcaggcc ccggccatcg ataacatcac
     2161 tcatggtggc ttggggagtg gccttcaagg cactgcccca ttgcccacgg ttgtagggct
     2221 cgcgggccgc gccagtctga aaaaatgtcg gaatagggtt atttgctgaa agccgagcgt
     2281 gaccagcgct tgggttgatc ggcggcacta gtcctttccc
//
LOCUS       DASUTG010000879         3951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1917978, whole genome shotgun sequence.
ACCESSION   DASUTG010000879 DASUTG010000000
VERSION     DASUTG010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917978"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF089_20475"
     CDS             <1..588
                     /locus_tag="VF089_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX6176390.1"
                     /translation="VAFCFGTLVGTGLPPAPTYIITVLVIAPFMIKADVDPWSVHFFA
                     FFVGVWGELTPPTSIVAAVTSKIAQASFMATMMKGIQICLPLFILMGAVFTRPELVLE
                     PGLQQVFAFVLVLIGTLGVVCSVHALYSENQSLDLTLRSALALLSIAAALAPSDVIAL
                     AAAVPVTLLVIYAFYRSRRMTLGGGGPRRPARLAP"
     gene            1377..2603
                     /locus_tag="VF089_20480"
     CDS             1377..2603
                     /locus_tag="VF089_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176391.1"
                     /translation="MNANVLDQKTPERWRALALLAVTLVLSMTTWFSASAVIPQLREE
                     WQLSATGAAWLTIAVQLGFVFGALVSSFLNLSDIISPKHVILGGTFGAAAANGLVEIA
                     GAASIGIALRFATGFFLAGVYPPALKLMATWFQRDRGVALGILVGAIVLGQAMPHLIN
                     GLGGLDWRVVIYTTSALTLTGGLITEFVVREGPFPFPKATFDPMQMWRVFANRGVRLA
                     SYGYFGHMWELFAMYAWFLLFFAEALRARGVTVGSAAAFATFAVIAVGALGCWVGGIL
                     GDRWGRTKTTALMMAISGSCCVLVGLLFSAPTWLLLLVGLVWGFTVVADSAQFSAMVT
                     ELADQSYVGTALTVQLAVGFILTVATIWLIPLTADTLGWRWVFVFLAPGPALGVLAML
                     RLKSLPESARLAGGVG"
     gene            2970..3335
                     /locus_tag="VF089_20485"
     CDS             2970..3335
                     /locus_tag="VF089_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176392.1"
                     /translation="MLKETTDGCLATILIYHQLFEEMLRLLIRAQFLIQLAVFPSRIA
                     FRDKRKQIFGQLQQELKDLVDFEGKSRFLAKAEQINAIRVGVVHKLTQRGSLAGLTRE
                     ARRAKRLYYDAFIILRSST"
     gene            complement(3387..3707)
                     /locus_tag="VF089_20490"
     CDS             complement(3387..3707)
                     /locus_tag="VF089_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin-B12 independent methionine synthase"
                     /protein_id="HEX6176393.1"
                     /translation="MFRKLKPQRLLLEFDDERSGSFEALREVPEDKIIVLGLITTKNP
                     ELESLASLKEKVTEATRYVPLERLAVSPQCGFASSIIGNNISPEDQRRKLALVTKAAG
                     AIWS"
     gene            complement(3768..>3951)
                     /locus_tag="VF089_20495"
     CDS             complement(3768..>3951)
                     /locus_tag="VF089_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6176394.1"
                     /translation="RLGATYIQIDAPHYGLLLDAKTRVFYENLGWSLNRWLSLGIELD
                     NAVVDGFREVTFGFHI"
BASE COUNT          767 a         1079 c         1137 g          968 t
ORIGIN      
        1 gtggcttttt gcttcggcac tctggttggc acgggactac cgccggcacc gacttacatt
       61 attaccgtac tcgtcattgc tccatttatg atcaaggcgg acgtggatcc atggagtgtc
      121 catttttttg ccttcttcgt cggtgtgtgg ggcgagttga ctcctcccac gtcaatcgtc
      181 gccgccgtca catcgaagat cgcccaagcc tctttcatgg ctactatgat gaaaggaatt
      241 cagatttgcc tgccgctgtt cattctgatg ggtgcggtat ttactcggcc ggagctggtg
      301 ttggagccgg gcctacagca ggtctttgcc ttcgtcctgg tgctgattgg aacattggga
      361 gtggtctgta gcgttcatgc gctctacagc gaaaaccaat ctcttgacct tacattaaga
      421 tccgctctgg cgctattgtc catcgccgcc gcgcttgcac caagcgacgt gatcgccctc
      481 gcagctgcag tgccggtgac gctgctggtg atttatgcct tttatcgctc taggagaatg
      541 acgcttggcg gcgggggtcc tcgaagacct gctcgactcg cgccgtgaaa aacgccaaag
      601 ctcgactccc cgacatttga gaaacgttca gcgtccagga aatcccagaa tgtcggttcc
      661 ccagtgccgt cgccagtgtc ggcaccgaaa tcataaacac tcgggtcgaa attccagcta
      721 tggtaacgcc gataacggaa ttgatcagag taagccggcg cacccgctca tctatgggtg
      781 attgcttttc gggtgattga ttttgcaagt tcaaagtggc gagcctcaca gcttagagat
      841 tttgctagat atccccgcgc acgatcacac gtaggccgtc cgacttgact gttatcgtgg
      901 cggagacttg cagaacgacg tcacctgcgg agatcgttcg attgtaccca gtcctagttg
      961 ccttttgtag gattgcgctg attaaatcta cataagtgag atcttttgtc gtcgatctca
     1021 acgaatggca tcgaggtctg ccttagacga ttgcacacac atgtattaaa tcgaggcgga
     1081 cgcggactgc gaagccctgc tgacagcttg ggttcagtac tagaagtgca accccgagga
     1141 gcctgaatac atggaaacaa ctttcagcct tagtcggatc gcgccgacgc aggagacttt
     1201 acaagccgtt gtcctcgttg taaaagcagc caaaataata tcgtttatga gaggtaatat
     1261 actgaagctg tgtcgcgcta tagggaagca gataattata gttagcctgc gcgcctgaca
     1321 atgccagagt aattcgccgt aacagggaat cggtgctttt ttcctggagc acgatggtga
     1381 atgcgaacgt gctcgaccag aagactcctg aacgatggcg cgcgctggca ctcctcgcag
     1441 tcacgctcgt tctgtcgatg acgacctggt tctcggcctc cgcagtgatc ccccaattgc
     1501 gtgaggagtg gcaactttcc gccactggcg ccgcgtggct gacaattgcc gttcaactgg
     1561 gctttgtgtt cggtgcgttg gtctcgagct tcctgaacct ctcggacatc atctcaccga
     1621 aacacgtgat cctgggcgga acgtttggtg ctgccgcagc gaacggactt gtggagatcg
     1681 ctggcgcagc tagcatcgga atcgcgctgc gcttcgcgac aggctttttt ctggcgggtg
     1741 tgtacccgcc ggccctcaag ctgatggcta cttggttcca gagagatcgg ggggttgcgc
     1801 tcggaattct cgtcggcgca atcgtcctgg ggcaagcgat gcctcacctt atcaatgggc
     1861 tcggtggcct ggactggcgc gtggtaatct acacgacgtc cgccctgact ctcactggtg
     1921 gcttgattac agagttcgtg gtgagggaag gccccttccc tttcccgaag gcaactttcg
     1981 atcccatgca gatgtggcgt gtgttcgcca accgcggcgt ccggctagcc tcctacggtt
     2041 acttcggcca catgtgggag ttatttgcga tgtatgcgtg gttcttactc ttcttcgccg
     2101 aggcccttcg cgcccggggc gtcactgtgg gttcggctgc ggcctttgcg acattcgcag
     2161 tcattgcggt cggggcactg ggctgttggg tgggtggcat ccttggagat cgctggggca
     2221 ggacgaaaac gaccgcgctc atgatggcca tctccgggag ctgctgcgtg ctggtagggt
     2281 tgctgttcag cgcgccgact tggctgctgc ttctagtcgg cctagtctgg ggcttcaccg
     2341 tcgtggccga ctcggcgcag ttctcggcga tggtcactga gcttgcggat caatcttatg
     2401 tgggaacggc tttgacagtg cagctggccg ttggctttat cctcacggtc gcgacgatct
     2461 ggctgattcc gttgaccgct gatacccttg gatggcggtg ggtgttcgtg tttttggccc
     2521 caggtcccgc tctcggcgtg ctggcgatgc tgcggctgaa gtccttgcct gagtccgctc
     2581 ggctcgcggg gggcgtcggc tgaacgtttc ccgaagcgaa cgagagcaca gccatgcagc
     2641 atgtcacgca gaacgcgccg cctaaccctg cgcttaacag gacgggccgg tacgtgtctc
     2701 caatttggcg agcgtcggcg cggcccgccg gttagcttga acgttaggat gacgacggac
     2761 gagcttcttg gacgatgtcg gcgtgactgt caaaaacttt ggatgagctg gtgtgttgaa
     2821 aaacgacggc gttggcattg agggtggcgg tggcggggat cgcgaagtat gaacggtagc
     2881 tacgtgcgcg cttgaaggac tcaggccagt ggccagcgtt ctcgtcggct acatttcttg
     2941 accgactcga tggtatcgca tcgcgggcga tgttgaagga gaccacggat ggctgtctcg
     3001 cgaccatcct gatctaccac caactttttg aagagatgct ccgtttactc attcgcgctc
     3061 agtttctcat ccagctggcg gtctttccct cacgtatcgc gttccgcgat aagcggaagc
     3121 agatatttgg tcagctccag caggagctga aagacttagt cgattttgaa ggcaagagcc
     3181 gttttttggc caaggcagag caaatcaacg cgattcgagt tggggtcgtt cacaagctca
     3241 ctcagcgtgg ctcacttgcc ggattaaccc gcgaagcacg tcgagccaag cgactttact
     3301 acgacgcgtt tatcatactt cgatcaagca catgacgact tccgcgtcgc tttccacggc
     3361 tacaagaagg atgtgtttga attgccctag ctccagatag cccctgcagc tttggtcacg
     3421 agggccagct ttctccgttg gtcttcaggc gagatgttgt tgccaatgat cgacgaagca
     3481 aaaccgcact gcgggctgac tgccaaacgc tctagcggaa cataccgagt cgcttcagtc
     3541 accttctctt ttagtgacgc caacgactca agctcaggat tcttcgttgt gatcaagccg
     3601 agaactatga tcttgtcttc aggcacttct ctgagggcct caaacgagcc cgagcgctcg
     3661 tcgtcgaatt ccaagagcaa tcgctgtggc tttaacttgc gaaacaattc gcggctgatc
     3721 gcttcatatc ctccctgcga cagccaccgg ctgccttggt tgccgcgtca gatgtgaaaa
     3781 ccgaaagtca cttcgcgaaa tccgtccacc accgcgttgt ccagctcgat gcccaaagag
     3841 agccaccggt taagactcca gccaaggttt tcgtagaaga ctcgcgtttt tgcatcgagt
     3901 aacaggccat aatgcggcgc gtcgatttgt atgtaagtgg cgccgagccg c
//
LOCUS       DASUTG010000880         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1917991, whole genome shotgun sequence.
ACCESSION   DASUTG010000880 DASUTG010000000
VERSION     DASUTG010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917991"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VF089_20500"
     CDS             complement(<1..961)
                     /locus_tag="VF089_20500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX6176395.1"
                     /translation="MARKNTKSHSDIVEKKLVGVADSVITAAQRSQDPTLSIPVRSLA
                     NVNFNEKRGIIEMGRRKQARSFFNVGMAKKFMQTVLVADALAELQRANLTTSLREIYY
                     RTKHTIKNSHENTFDGQNESDPLIEDLEVTLEALREELHVRAENSGTVVGPLVLVDDG
                     DRVDCTRLGKGGYSVPSIVEPEYIQISKCTADFVLLVEKGTQWNRLSEDKFWRRYNCI
                     LLTGNGQPPRGVRRLARRLHQEYKLPVYVLVDNDPWGYYIYSVIKQGSINLAFESERM
                     AIPKAKFIGFSSADPDRYGLPRNVGIKLNDKDINRARELLKYKW"
     gene            complement(970..>2983)
                     /locus_tag="VF089_20505"
     CDS             complement(970..>2983)
                     /locus_tag="VF089_20505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX6176396.1"
                     /translation="AQVKSNDALEISPAAAKISLSAVEMGARQREISVSEFFTKNRHL
                     LGFDNPRKALLTCVKEAVDNALDACEEAGILPEVTVRLEVASNGNAAPPPSQANRFRI
                     TVIDYGPGIVRQQVPKIFAKLLYGSKFHRLRMSRGQQGIGISAAGMYAQLTTGKPVQI
                     TSRISAKSPAHYFEVQIDTKKNEPRILENKKIEWENHRGTQVTLEIEGRYQKGRASVD
                     EYLEQTAIANPHVKLVYHNPEGETKEYPRTLQELPPQPREIKPHPYGIEFGILLRMLH
                     DTKSQSLGGFLSSDFSRVSSHLAQEICKTAKLSPGIRPRNVHGSAAEALFKAIQSTKI
                     MAPPSNCISPIGERAILHGLYKQIKGEFYTAVTRPPAVYRGNPFLIEAGLAYGKAPEQ
                     APEKPAQPRVALAEGEQEENDNELARVIRYANRVPLLYQQSACSTFKAVLDTTWRNYG
                     VPQSRGALPAGPMVIFIHMASVWVPFTSESKEAIADYDEIRKEIKLALQECGRRLGVF
                     LRRREHAKSEFRRRNIFELYIEEVVEACNRLKGGKLPKEKLKEQLQKIAAKRTGGQKT
                     DEALGRNAGPEGLPFSIIVTPEGTEGDAPVIAGDERSELAPPEEAREDEPLAAAIELD
                     ASTESLAQVKKKTAQRKQQSASKTESARKAKAVSKKPRTASKPSKK"
BASE COUNT          582 a          814 c          820 g          767 t
ORIGIN      
        1 accacttgta cttcagcagc tcgcgcgccc ggttgatatc tttgtcattg agtttgatgc
       61 cgacattgcg cggcaagccg taacgatccg gatcggcgct cgagaaaccg atgaacttcg
      121 ccttcggaat ggccattctc tcgctttcga aagctagatt gatcgagcct tgcttgatca
      181 ccgagtagat gtaatagccc catgggtcgt tatctacgag aacataaacc ggaagcttgt
      241 attcttggtg aagccgccgc gccaagcggc gaacgccgcg cgggggctgg ccgttaccgg
      301 tcagcagtat gcagttatac ctgcgccaga acttgtcttc ggaaaggcgg ttccactgag
      361 tgcccttttc aacgagcagg acaaaatcag cggtgcattt gctgatctga atgtattcgg
      421 gttcgacgat agagggaacc gaatacccgc ccttgccgag cctggtgcag tcaacgcgat
      481 caccatcatc gaccagcacc agcggtccaa cgaccgtccc cgaattctcg gcacgcacat
      541 ggagctcctc acggagcgct tccaacgtga cttcaagatc ctctattaac ggatcggatt
      601 cgttctggcc atcaaatgta ttttcgtgcg agttcttaat agtatgcttc gtgcggtaat
      661 agatttcccg aagcgaggtg gtcaggttgg ctcgctgcag ctcggccaag gcatcggcga
      721 cgagcaccgt ctgcatgaat ttcttcgcca tgccgacatt gaagaacgag cgcgcctgtt
      781 tgcgccggcc catctcgatg atgccgcgct tctcgttgaa attgacgttg gcgaggctgc
      841 gcacgggtat cgaaagggtg ggatcttggc tgcgttgcgc cgccgtaatt actgaatcgg
      901 cgaccccaac cagcttcttc tctacaatat cgctatgact ttttgtgttt ttgcgggcca
      961 tgtcttttcc tattttttgc tgggcttcga tgcggttcgg ggcttcttag agacggcttt
     1021 cgcctttcta gcgctttcag ttttggaagc gctctgctgt ttgcgttgtg ctgtcttttt
     1081 ctttacctgc gccagagact cagtgctagc atccaattcg attgctgccg ccaacggttc
     1141 gtcttcgcgc gcctcttccg gcggcgcgag ttcgctcctc tcgtcgcctg caatcaccgg
     1201 agcatctcct tccgtgcctt ccggtgtgac gataatcgaa aaaggaagac cttccgggcc
     1261 ggcgttgcgg ccgagagctt cgtccgtctt ttggccgccg gtgcgtttgg cagcgatttt
     1321 ttgcaattgc tctttgagtt tttctttggg cagctttcca cccttgaggc gattacaggc
     1381 ttcgacaact tcttcgatgt agagctcgaa tatattgcgg cggcggaact cactcttggc
     1441 gtgctcgcgg cgtctaagaa ataccccgag ccggcgacca cactcttgca gagcaagttt
     1501 gatttctttt cggatttcat cgtaatcagc gatcgcttct ttggattcac tggtgaaagg
     1561 cacccaaact gaagccatat gaatgaagat caccatggga ccggctggca gagcacctct
     1621 agattgagga acgccgtagt tccgccaggt ggtatcgagc acggccttaa aggtcgagca
     1681 cgcagactgc tggtataaga gcggcacccg gttagcgtaa cgaatcacgc gcgccagctc
     1741 attatcgttt tcttcctgct ccccttcagc aagtgcaacc ctcggctgtg cgggtttttc
     1801 cggggcctgt tccggcgcct tgccataagc caggccggcc tcgatcagga aagggttgcc
     1861 ccgatacacc gcaggcggcc gggtgacggc agtgtagaac tctcctttga tttgcttgta
     1921 gagaccatgg aggatcgccc tttcaccgat gggcgagatg cagttgctcg gcggcgccat
     1981 aatcttggtc gattgaatcg ctttgaaaag agcctcggca gctgatccat gaacatttcg
     2041 cgggcgaatg ccgggcgaca gctttgctgt cttgcagatc tcctgagcga gatggctcga
     2101 gacccggctg aaatcactgc tcaagaatcc accgagcgac tggcttttag tgtcgtgcag
     2161 catcctgagt aaaattccga actcgatgcc gtaggggtgc ggctttattt cacgcggttg
     2221 cggcggcagc tcctgaagtg tccgcggata ctctttagtc tcaccctctg gattgtgata
     2281 aaccagcttc acgtgcgggt tggcgatggc ggtctgctcc agatactcat cgaccgaagc
     2341 ccgtcctttt tgatatcgcc cttctatttc gagagtgacc tgagtgccgc gatggttctc
     2401 ccactcgatc ttcttgttct caagaatacg gggttcgttc ttcttggtat cgatctgaac
     2461 ctcgaaataa tgagcaggag acttggcgct gatgcgagaa gtgatctgca ccggtttgcc
     2521 cgtggtgagt tgcgcataca tcccggcagc cgatataccg atgccctgtt gtccccggct
     2581 catgcgcaac cgatgaaatt tggatccgta gagaagcttg gcaaaaattt ttggcacctg
     2641 ctggcggaca atacccgggc cgtaatcgat cacggtgatt cgaaatcgat ttgcctggct
     2701 gggcggcggt gcagcatttc cattactagc gacctcgagc ctgaccgtaa cttcgggtaa
     2761 gattccggct tcctcacagg cgtccagcgc gttatcgaca gcttctttga cgcaggttag
     2821 tagggccttc cttggattgt cgaagcccaa caagtgacgg tttttggtga agaactcgga
     2881 aacggagatt tctctctggc gggcgcccat ctctaccgca gatagggaaa tctttgcggc
     2941 agcgggcgaa atttcgagtg cgtcgttcga ctttacttga gct
//
LOCUS       DASUTG010000881         4826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1028243, whole genome shotgun sequence.
ACCESSION   DASUTG010000881 DASUTG010000000
VERSION     DASUTG010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028243"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..561
                     /gene="gspG"
                     /locus_tag="VF089_20510"
     CDS             124..561
                     /gene="gspG"
                     /locus_tag="VF089_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX6176397.1"
                     /translation="MIYHSKKYLNNPRGFTLVELIVVVIIIGLLAGLVLPQFIRQEEK
                     AKLRTTKAQIELFGTALDTFRLDVGRYPTTDEGLQALRQKPGGLERWDGPYLRKDLPL
                     DPWSKPYAYRSPGEHGPYDIVSYGADGTPGGDGDNRDITSWEK"
     gene            536..997
                     /locus_tag="VF089_20515"
     CDS             536..997
                     /locus_tag="VF089_20515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6176398.1"
                     /translation="MILLAGKNSRGFSLLELILVLMVLGLSGLIVLPNVEKGLRDRDL
                     RQSALALAAAARDLRSQALHKGVPQGLVLNVSESSYRTARNEVHFPSSVIITSVAGGE
                     VLDDGSRHFLFFPNGSTFGGRIDLSSGADSTSYSIRLHPLTGTIDVRRGDS"
     gene            997..1407
                     /locus_tag="VF089_20520"
     CDS             997..1407
                     /locus_tag="VF089_20520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6176399.1"
                     /translation="MLDKSCLGSGRGFTLLEVVVAMAIVGVGVVTLLEIFSSGLQLGA
                     RSRDRTEAVAYGRQIMDQFLARRTLLEGTEQGTIGETNRWQLQVQPVRPVEGLTLGND
                     WELKEIRLEIFVPESGRERRFELRTLRLVKKGDR"
     gene            1407..2045
                     /locus_tag="VF089_20525"
     CDS             1407..2045
                     /locus_tag="VF089_20525"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX6176400.1"
                     /translation="MKTLRHPRNSPGFTLIEVVLALSIFALIGAVLYGAFSLSHSAVA
                     KSQASFENNQKLRSFTDLLGSYIRSAHPYRQSAQDTTVFFDGQEEQLTFVSSISLAMG
                     GRGMSKIRVAWEGAEEGEGPIELEEEVPVRLNLSEDLNPGGLRNELVIQERVKELRFA
                     YLDPKSEEEIWEERWDGREKLALPRAVRLSYRDHNGKEIRWVFPLMMSVLAQ"
     gene            2060..3067
                     /locus_tag="VF089_20530"
     CDS             2060..3067
                     /locus_tag="VF089_20530"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176401.1"
                     /translation="MKTKLAGVTNERGVALLIVLWIFIFLFVVAFDFSTGVREEGTAA
                     HRYSDDNQGYYLALAGFEQGLYDFLSQSRGRELKAVDKPKDIFDAGWREATLGGGTYR
                     LRLVDESSKINLNRADETMLRRIFSNLGIDEPLIGVLIDSIMDWRDQDDLHRSNGAED
                     DYYLSLSPPYTAKNGPFDSLEELLWVKGVTASLYYGYDSSGTPPGDSPRVGLREIFSV
                     DSPIDRVNLRTASAAVIHVLLGIPLEKCQGFVEERTKLGDKTLADLLPLLGVGSGDAA
                     LQMFIFTNPSIVTVEAEGYASESRQPRRVKGVIRAGGGNRGYELTRWVDREIGYPMGR
                     Q"
     gene            3132..4682
                     /locus_tag="VF089_20535"
     CDS             3132..4682
                     /locus_tag="VF089_20535"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX6176402.1"
                     /translation="MNLSDSLRNLPRADFLRSVGIYIMRDHLVLVRLRKNFLSVTLLE
                     QELRDLPLGENRQAISELTGWIAEDVREIALKAEHDSRERALRQAILSLLPHFSAGRD
                     SLYICVPQDQAIVQTVYLPQAAESNLQQVLEYEIERQLPLSRDEIYYDYLPMGKRGDK
                     IGVYLFAIPKKDLAGVLDVLGSFGIVPSGVETTATALANYLLFCKKDLAGSAAIVGGH
                     ADDREIIGVRASAGGWTQAPELLFSHRVPDAEWAEGIGKELIAECLRQTPKLYGWGDA
                     ERVFHSADGEKVECEDLCILANERLGGGAHLSAATLPAVGAALRGVREARFETNVLRP
                     AGADRGARNSLSLMNSVMVGLLVLLLLGWGISYPIKDELRLKQLQQENQKTEPSVEAL
                     RREEAQLQTVRKEVQFLSDIHRRKGEVLRVLDELSKVVPTSAYLSNFRYRNGVLEVQG
                     NAENASTLIPLLEKSPLFENVAFNAPSNRGRDNRETFSLKAELEKQAYTGSKSPSSDK
                     PNDKAGKL"
BASE COUNT         1101 a         1162 c         1406 g         1157 t
ORIGIN      
        1 atcaagaggt aaagcgaaca acaaaaagat taacatccat catcgagccg gtgttgatct
       61 tgggtatggg cctggtgatc ggtgtcgtgg tcatctccat gttgctggca atttttagta
      121 tcaatgattt accattctaa aaaatatctt aacaatccca gagggttcac gctggtcgag
      181 ctgatcgtcg tcgtgattat catcggactc cttgctgggc tcgtgctacc gcagttcatt
      241 cgtcaagaag agaaggcaaa gctcaggaca accaaggcgc agattgagct ctttggaacc
      301 gccctcgata cctttcgttt ggatgttgga cgctacccga ccactgatga aggcctgcaa
      361 gcgcttcgac aaaaaccggg aggattggag cgttgggatg gtccgtatct gaggaaggat
      421 ctgccgttgg atccgtggag taagccttac gcttacagaa gtcccggaga gcacggcccc
      481 tacgacattg tatcctacgg cgctgacggt acacccggcg gcgacggcga taatcgtgat
      541 attactagct gggaaaaata gtcgcggctt ttctctgctc gagctcattt tagttctgat
      601 ggttctcggt ctgagtgggc tcatcgtcct accaaacgtg gagaaaggct tgcgagaccg
      661 ggacttgcgc cagtccgcgc ttgctttggc agcggctgcc cgcgatttgc gcagccaagc
      721 gcttcacaaa ggggtgccgc aaggattggt tctcaacgta tcagagagta gttatcggac
      781 cgcgcgcaac gaagtccatt ttccttccag cgtaataatt acatcggttg ccggcggaga
      841 agttctggat gacggaagcc gtcacttctt gttctttcct aatggcagca cgttcggcgg
      901 ccgaattgat ctttctagcg gtgcagattc aacctcttac tcgatccgac ttcacccgct
      961 caccggtact atcgacgtga ggcggggcga ctcgtgatgc tggacaaatc atgcttaggt
     1021 tctggccggg gcttcactct gctcgaggtc gtggtcgcca tggctatcgt cggagtcgga
     1081 gtggtgaccc tgctggaaat attttcctcg ggtttgcaac tcggcgctag aagtcgagat
     1141 agaactgaag cggttgctta tggccgtcaa ataatggatc agttcttggc gcggcgcacg
     1201 cttctagaag gtaccgaaca gggcacaatc ggggagacta accgctggca gcttcaggtt
     1261 cagccggtgc gacccgttga agggctaaca ttgggaaacg attgggagct gaaagagata
     1321 aggcttgaga ttttcgtacc ggaatcaggt cgggaaaggc gttttgagct gagaacgctg
     1381 cgtctcgtga agaaggggga tcgctgatga aaacattgcg tcatcctcgg aactcacccg
     1441 gctttacctt gattgaagtc gttctcgcat tgagtatttt tgctctcata ggagccgttt
     1501 tgtatggtgc cttctcacta agtcatagtg ctgtcgcaaa gtcgcaggct agcttcgaaa
     1561 ataatcaaaa gctgcgatcg ttcacggatc tgctcggcag ctatattcgt tcggcgcatc
     1621 cttatcgaca atcggctcag gacacgacgg tattttttga tgggcaggaa gagcaactca
     1681 cgtttgtttc ctcaatctcc ttggccatgg gaggtcgagg aatgtcaaag attcgggttg
     1741 cctgggaggg tgccgaagag ggcgaaggtc cgatcgagct cgaagaagag gtgccggtgc
     1801 gtttaaacct aagcgaagat cttaaccccg gcggtctgcg aaacgagttg gtaatacagg
     1861 agcgggtgaa agagcttcga tttgcttact tagaccccaa aagtgaagag gagatatggg
     1921 aggaacgctg ggatggtaga gaaaagctgg cgctgccgcg cgccgttcgc ctcagctatc
     1981 gtgatcataa tggcaaggaa attcgctggg tctttccgct gatgatgagc gttttggcgc
     2041 agtgaagatg gtgcagttct tgaaaacgaa gttagccggc gtaacaaacg aacgtggcgt
     2101 tgcacttctc attgttttat ggattttcat ttttcttttt gtagtggcct ttgatttcag
     2161 caccggtgtg cgcgaggagg gcaccgctgc tcatcgctac agcgacgaca atcaaggcta
     2221 ttatttggca ctggcaggct tcgagcaggg attatatgac ttcctcagtc aatcccgggg
     2281 acgtgagtta aaggcagtcg ataagccaaa agatatcttc gacgccggct ggcgcgaggc
     2341 gactctcggg ggcggtactt atcgcctccg acttgtggac gagagcagca agataaacct
     2401 caaccgagca gacgagacta tgctgcgccg cattttttca aatctcggca tcgatgaacc
     2461 tctcattgga gttttaatag attcgattat ggattggcgc gatcaggatg atcttcaccg
     2521 gagtaacggc gcggaagacg actattatct atctctcagt cctccttata ccgctaagaa
     2581 cggccccttc gacagtctcg aggagttgct gtgggtcaaa ggtgtaaccg cgagccttta
     2641 ctacggctac gatagcagcg ggactccgcc tggcgattcg cctcgtgtag gtctaagaga
     2701 gatctttagc gttgacagtc cgatcgaccg agtcaatctc cgcaccgcct cggcggcggt
     2761 tattcacgtt ttgctcggca taccgttgga gaagtgtcag ggattcgtcg aggaaagaac
     2821 gaagctcggc gataagacac tcgccgatct gctgccgctt ctgggcgttg gctccggcga
     2881 tgcggcattg cagatgttta tcttcacaaa ccccagtatc gtgacggtcg aggctgaagg
     2941 ttatgcgagt gagtcgcgcc aaccgcggcg cgttaaagga gtgattcgcg ccggcggcgg
     3001 aaatcgagga tacgagctga cccgctgggt ggaccgagaa attggctatc ccatggggag
     3061 acaatgaggt ataataggca acttttgtct gcaagtggca aagttccagc acaattcaaa
     3121 gagtttaaat catgaacttg tctgattcgc ttagaaatct tccccgcgcc gactttctca
     3181 ggagtgtcgg gatttatatc atgcgcgatc atctggtctt ggttcggctg agaaagaatt
     3241 ttcttagtgt cacgctgctt gagcaggagc tgagggatct accgctcggt gaaaaccgcc
     3301 aagcgatctc ggagttgacc gggtggattg ccgaagacgt gcgtgagatc gcgctgaaag
     3361 ccgaacatga ctcccgcgaa cgagcattgc gccaggcgat cctgtcgctc ttgccgcatt
     3421 tcagcgcggg cagggattcg ctttatatct gtgtcccgca agatcaggcg atagtccaaa
     3481 cggtttacct gccgcaagcg gccgagtcaa accttcaaca ggtgctggaa tacgagattg
     3541 aacgtcaatt acccttaagt cgcgacgaaa tttactacga ttacctgccg atgggaaagc
     3601 gcggggataa gatcggtgtt tacctgtttg ccattcctaa gaaagattta gccggcgtac
     3661 tggacgtctt gggatcgttt ggtatcgttc cgagcggagt tgaaaccacg gcgaccgctt
     3721 tagcgaacta cttgcttttc tgcaaaaaag acttggccgg ttccgcggcg atcgtcggcg
     3781 gccatgccga tgatcgcgaa ataatcggag ttcgggccag cgctggcgga tggactcagg
     3841 cgccggaact gcttttctca cacagagtgc ccgacgctga gtgggccgag gggatcggca
     3901 aggaactcat tgccgaatgt ttacgtcaaa caccaaaact ctacggctgg ggagacgcgg
     3961 aacgcgtctt tcattctgcg gacggcgaaa aggttgagtg cgaagatctt tgtattcttg
     4021 ccaacgaaag acttggcggc ggcgcacatt tatccgctgc aacgctgccg gcggtcggcg
     4081 cggcgctgcg gggcgttcga gaagcgcgct ttgaaaccaa cgttcttcgg ccggctgggg
     4141 cggatcgagg cgcgcgaaac agtctctcgt tgatgaattc agtgatggtg ggcttgctcg
     4201 ttctcttgct tctgggctgg ggcattagct acccgatcaa ggacgaattg cgtttaaaac
     4261 agctgcagca ggagaatcaa aagacggaac cttctgtgga agctttgcgc cgggaggagg
     4321 cccagctaca gacggtgcgc aaggaagtgc agtttctctc ggatattcat cggcggaagg
     4381 gcgaggtgtt gcgcgtattg gatgaacttt caaaagtggt gccgacgagc gcttatctat
     4441 ctaatttccg ctatcgcaac ggtgtcctgg aagtccaggg caatgccgag aatgcgtcta
     4501 cattgattcc gctattagag aaatcgccgc tctttgaaaa tgttgcattc aatgcgcctt
     4561 caaaccgcgg ccgtgacaat cgggaaactt tttcgctcaa agccgaattg gagaagcaag
     4621 cctacaccgg ttcgaaatcg ccctcgtctg acaagccgaa cgataaggcg ggtaagctat
     4681 gaaacaggtt tggggaaggt tatcgaaacg ggaacgcggt ttggttctga ccaccgtgct
     4741 gattctatta ctcgtgcttg ggcgtatctc agtgattggt ccctacatgg ctcatcgtga
     4801 gtgggtacaa actcagctgg aaatcc
//
LOCUS       DASUTG010000882        10650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1471383, whole genome shotgun sequence.
ACCESSION   DASUTG010000882 DASUTG010000000
VERSION     DASUTG010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471383"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF089_20540"
     CDS             <1..482
                     /locus_tag="VF089_20540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176403.1"
                     /translation="LRPMPSVAIIPVAVLLFGINDAMIVAVTVYASIWPVLINTIDGV
                     RHLERTLIDTGRTFGLNRRQILWWVVLPAAAPYIVTGLRISLSIALILVTTAEMIAGS
                     KGLGFFILDQERSMNSGNMYAGIILVSVLGYLLNRLFVLCEGWAMNWRRGMLARESI"
     gene            484..1257
                     /locus_tag="VF089_20545"
     CDS             484..1257
                     /locus_tag="VF089_20545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176404.1"
                     /translation="MRATKLNGLLFFLGLLIVWELVAQGGLINPLIVPPLSRILRVFS
                     ELVWSGQIPLQVAASMKRAAAGYVMAALVFIPLGILMGLSQTVYRLFEVIVEMLRPVP
                     PPVMIPVAMLFFGLEDEMKIFVIFFSCAWPILINTLDGARNVDPVLLNTARTFGLSQW
                     KIIWNVILPAAAPQIVTGLKVSLPITLILVVISEMVGSTDGIGYFILDSQRRFRVTHM
                     YAGMLTLAILGYTLNQSFNLLYKLVMPWHVGLMRMKDAE"
     gene            complement(1348..2259)
                     /locus_tag="VF089_20550"
     CDS             complement(1348..2259)
                     /locus_tag="VF089_20550"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6176405.1"
                     /translation="MKILRFDDDRIGVIKNDDKVVDVSDAISARKAKGGQRVMEEIIE
                     GWRKYRRRFEKIMNEQEGVALSSVQLRAPLPRPSKCIAAFSNYIDRPERTADNFPNEY
                     FYKSPDLVGPDGAVELTDIPAAVVYQPEAELAFVIGKPARNVSESKALDYVFGYVPFF
                     DISTRGMVRRTQFVPKGQDTHGCVGPWIITKDEIPDPHNVNVKSWTNGEPRQNYNTSA
                     MAHKIPNQIAWLSRFVKLNPGDIVATGTFHEGLKPVNSGDTIEIEFANMGRAKFQIKG
                     NSPRKDAEWLPGKNQPQPPPGGGMHRV"
     gene            2454..3041
                     /locus_tag="VF089_20555"
     CDS             2454..3041
                     /locus_tag="VF089_20555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6176406.1"
                     /translation="MNLVLRKYSDADVEDCVSVLADAFVSSPLHLSAFGNDRINQNRL
                     FFRLALRNMFFGRAFVALDDGALCGYVHFRAWPYCLPAPEEIPTAAATLLKPLGEAVP
                     KFIQWFARWCHLDPQEPHVHLGPIGVAPGKQRQGIGSALMNSYIAALEQERSLGYLET
                     DRLDNVEFYKKFGFVVTHEETVIGTPTWYMSRPVK"
     gene            complement(3976..4893)
                     /locus_tag="VF089_20560"
     CDS             complement(3976..4893)
                     /locus_tag="VF089_20560"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6176407.1"
                     /translation="MKVTLIHNPDAGSDDATCGEEPVELIRRAGHQLVYQSSKDSHWQ
                     RALDKPADLVAVAGGDGIVGTVAEIMVGRGIPIAVLPLGTANNIAKTLNLMDYSLAQL
                     IDGWSAAGRKKLDSGVANGPWGSAPFVEGLGIGLFTDMMTRLDARGNVDLAHLTEPEE
                     KITSVLEMLQARLLTCPEIELHVTVDGRDLSGEYILLEVMNIKSIGPNLFLAPDADPG
                     DGLFDVVCLTRGAHDTLDRCLEECKEGSRAPTGLPVRRGKQIHIEWEGTPIHIDDRAW
                     PENGANFPPSLTVIDITVEPHSLEFLTPA"
     gene            4993..5730
                     /gene="surE"
                     /locus_tag="VF089_20565"
     CDS             4993..5730
                     /gene="surE"
                     /locus_tag="VF089_20565"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX6176408.1"
                     /translation="MRILVANDDGIYSPGIAVLAEVASKFGEVRVVAPDVERSSAGHS
                     ITAMRPLSYRRTPIGKFEAYRVDGTPADCVALGIFHWEKVDVVLSGINLGSNLGNSIW
                     HSGTLAAAKQATLLGLRGIALSSTVTIDEPNFDLLKPSLEEVLTLLLSDNKDFCLVNV
                     NFPDEKPKGLRWTRQSVRHYDGKIVTGEDPLGRKHFWFSVVPVEETEEGTDRWAVEQG
                     YVSLTPLRLDLTNEKELAAALARYPLA"
     gene            5905..6240
                     /locus_tag="VF089_20570"
     CDS             5905..6240
                     /locus_tag="VF089_20570"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6176409.1"
                     /translation="MRFSHCYCSRCRKATGGVRATNIAVPIDQFRWTRGEELIARYDL
                     PEAKSFARQICTRCNCPVPHASRDRQRVIVPAGTLDDELTTKPTSHGHWSSRVSWAEI
                     DESDLPCSD"
     gene            6363..6803
                     /locus_tag="VF089_20575"
     CDS             6363..6803
                     /locus_tag="VF089_20575"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6176410.1"
                     /translation="MELTVWRPFAGLESFDSQINQLFDQVLGQAWIGSRSASTGGYPA
                     VDVLESKDSYLLRAELPGMNKEDFNLEVKNGMLTISGERRPEELSSGVRYRSVERVAG
                     RFSRSFYLPETVQQDGIQATYRDGILEIYVPKLEHAKARQIRVS"
     gene            6933..7469
                     /locus_tag="VF089_20580"
     CDS             6933..7469
                     /locus_tag="VF089_20580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6176411.1"
                     /translation="MTKILGTITCFLLLGVLLAVALPIKHPILAVQLTFAEPPASLPV
                     PVDGVRPSAIRNSWRAPRDGGRYHQGIDIFAKPGTPVRSTTEGIVIRLGQNRLGGNVV
                     WIMGPGRQKHYYAHLSHFGAFEIGDRVLAGDIIGYVGDTGNALGTPHHLHYGVYELGG
                     SAINPFPLLTARANQERA"
     gene            7595..8812
                     /locus_tag="VF089_20585"
     CDS             7595..8812
                     /locus_tag="VF089_20585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6176412.1"
                     /translation="MPFSKLGLSPKVVDGVRAAGYTDPTPIQLRAIPILMEGRDLIGS
                     AQTGTGKTAAFALPIISRLGQRRALRALILEPTRELAAQVETAIRDYGRFTNLRTVVL
                     YGGTGYGKQDQTLRRGADIVVATPGRLLDQISRGMLRLDQIEILVLDEADRMLDMGFM
                     PDVRKIVEKCPRNRQTMLFSATVPPEIDQLCKWALNKPEKIEIGQRRSPAATVTHALY
                     PVDSEQKQELLEELLRRTDYDQVLIFCRTKNGADRVVRKLHQQGHAVAVLHSRRTQRE
                     REHALDGFRNGRYEVMVATDIAARGIDVAQISHVINFDVPHHPEDYVHRIGRTGRAQS
                     VGDAFTIMTGEDVQEIAAIERFIGQKIPRVKLENFKYSYTRLLDPNPKPIFGRGRGQG
                     APGKRSFGYGRRR"
     gene            9011..9442
                     /locus_tag="VF089_20590"
     CDS             9011..9442
                     /locus_tag="VF089_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6176413.1"
                     /translation="MSSTIDFERLVQAAGDAIIAADPEGRIVSWNPAAERIFGFTAQE
                     ALGQTLDLIIPERFRARHWDGYKQVMRTGQTRYGTEILRVPARHRDGRPLSIAFTVAL
                     LSRADGQTEAIAAIVRDETSRWNEERSLRERLRNLEEQQSK"
     gene            9509..10012
                     /locus_tag="VF089_20595"
     CDS             9509..10012
                     /locus_tag="VF089_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6176414.1"
                     /translation="MNVVWEELAAGFPDAETMVRITIRLIVAMIFGAVIGIQRERAGK
                     PAGLRTHMLVSSGAAVFLIACGEFGMDPDSISRVIQGLVTGIGFLGAGAILKLYERRA
                     VEGLTTAAGIWMTAALGVAVGLGRFGLARMATLLAWMTLSLVRQLEHMLNRGIRKSEE
                     DTISAED"
     gene            complement(10337..10633)
                     /locus_tag="VF089_20600"
     CDS             complement(10337..10633)
                     /locus_tag="VF089_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176415.1"
                     /translation="MDWLDALQWPAMVVTVSAAWLIGSQRKIRRMIGFWCFLLSNALW
                     IIWSITIAQARACQPQMIQSTLLTKKHQKPIMRRIFRCEPMSQAALTVTTIAGH"
BASE COUNT         2282 a         2896 c         2940 g         2532 t
ORIGIN      
        1 tcttgcgccc catgccttct gtggcgatca ttcccgtagc ggttttgttg ttcggcatta
       61 acgatgcgat gatcgtggcg gtcaccgtct acgccagtat atggccggtc cttatcaata
      121 ccatcgatgg cgtgcggcac cttgagcgga cgttgatcga caccggcaga acctttggtc
      181 ttaatcgccg acaaattctt tggtgggtgg tgctgccggc ggcagcgcct tatatcgtga
      241 ctggtctgcg catcagtctg tcgattgcct tgatcctggt tactactgcc gagatgatcg
      301 ccggcagcaa ggggctggga tttttcatcc ttgatcaaga gcgctccatg aacagcggca
      361 acatgtacgc cggtatcatt ctcgtttcag tcctgggata tctgctcaac cgattgtttg
      421 tgctttgcga aggttgggcg atgaattggc gccgcggcat gcttgcccga gagtcgatct
      481 gacatgcgcg caacgaaact taacggcttg cttttctttc tcggcctgct gatcgtctgg
      541 gaactggtcg cccaaggcgg attgatcaat ccgttgattg ttccgccctt gagcaggatt
      601 ctccgagttt tctcggagtt agtgtggtcg ggtcaaattc ctttacaagt cgcagccagc
      661 atgaaacggg cggcggcagg atatgtgatg gctgcgctag tgtttatccc gttgggaatt
      721 ctgatgggtc tttcgcagac tgtttaccgc ctctttgaag tcatcgtcga gatgcttcgg
      781 ccggtgccgc cgcctgtgat gattccggtg gcgatgctgt tcttcggctt ggaagatgag
      841 atgaaaattt ttgtcatttt cttttcctgc gcctggccga ttctaatcaa taccctggac
      901 ggcgcgcgta atgtcgaccc ggtgctgctc aacacggccc gtacttttgg attatcgcag
      961 tggaaaatca tctggaatgt tattctgccg gcagccgcgc cgcagatcgt taccggattg
     1021 aaagtcagcc tgccgatcac gcttatcctc gtggttatct cagaaatggt cgggagcacc
     1081 gacgggatag gttactttat tctcgattca caacggcgat ttagagtaac gcacatgtat
     1141 gcggggatgc ttaccttggc gattctcggg tacacactta atcaatcttt caatctgctc
     1201 tacaagttag tcatgccgtg gcatgtgggc ctgatgagga tgaaggatgc cgagtaaaga
     1261 atggagtatt ggagtggtgg agtagtgggt ccggataaac ccaccagtcc agtactccat
     1321 cgctccacag gcatctcaca tgttctttta gaccctatgc atgccgccgc ccggaggagg
     1381 ctgcggttgg tttttgcccg gcaaccactc cgcatccttg cgcgggctgt tgcctttgat
     1441 ttgaaacttt gcgcggccca tgttggcgaa ttcgatctcg atcgtgtcgc cggaattcac
     1501 cggcttcaat ccttcgtgaa aagtgccggt ggcgacgatg tctcccggat tgagtttcac
     1561 gaaacgcgaa agccaggcaa tctgattggg gatcttgtgc gccattgcgc tggtattata
     1621 gttttgacgc ggttcgccgt tggtccacga ttttacatta acgttgtgcg gatcgggaat
     1681 ctcgtccttg gtgatgatcc acggcccgac acagccatgg gtatcctgcc ccttgggaac
     1741 aaactgcgtg cggcgcacca ttccccgggt cgaaatatcg aagaaaggca catagccgaa
     1801 gacgtaatcc aatgccttgg actcggagac atttctagcg ggcttgccga tgacgaaagc
     1861 gagctcggct tcgggttgat aaactaccgc ggcggggatg tcggtcaatt ccacggcacc
     1921 gtcaggcccg acaaggtcgg gagatttgta gaaatattcg ttggggaagt tatcggccgt
     1981 gcgttccgga cgatcaatat aattgctgaa ggctgcgatg cacttgcttg gacgtgggag
     2041 gggagcgcga agctggacgc tgctcaacgc cactccttcc tgctcgttca taatcttctc
     2101 aaagcggcgc ctgtatttgc gccagccttc gatgatttct tccattaccc gttgcccgcc
     2161 cttggctttg cgcgcgctga tggcatcgct gacgtcgaca accttatcgt catttttgat
     2221 gaccccgatc ctgtcgtcgt caaatcgtag aatcttcacg gtgtgctcct ccagtgcaaa
     2281 tttgcgccta tatttgcgtc aaccgcagag taatgtcaaa ccaggccagg tgatctaaag
     2341 cagagcaagg cagtcggacg gccatcccgg atcggaagag acaatcattt cggagcgctg
     2401 atgttttctc cgcgaaaaag ctcgcgtaaa ctttaggcag gagtttttgt tcgatgaacc
     2461 tggtgctgag gaaatactcc gatgctgatg tggaggattg cgtgtcggta cttgccgatg
     2521 cgttcgtttc cagcccgttg cacttatcgg cgttcggcaa cgatcgtatc aatcagaatc
     2581 ggctgttctt tcggctcgcg ctgcgcaata tgttttttgg tcgagccttc gtcgcgttag
     2641 atgacggcgc cctgtgcggc tatgttcatt ttcgagcatg gccatactgt ctgcccgcgc
     2701 cggaggagat tcctaccgcg gccgcgacac tgttgaagcc tctcggcgaa gcggtgccga
     2761 agttcattca atggttcgcc cgatggtgcc accttgatcc acaggagccg cacgttcatc
     2821 tcggacccat tggcgtggct ccaggtaaac agcgacaggg aatcggcagc gcattgatga
     2881 attcctacat cgcagcactc gagcaggaaa gatcgctcgg ttatcttgaa accgatcggc
     2941 ttgacaacgt cgagttctac aaaaaattcg gcttcgttgt gacgcatgag gaaacggtca
     3001 tcggcacgcc cacctggtac atgtcgagac ctgtcaaata acggtgacga cgattctgag
     3061 gattcgctgc agaaacttca gcgcttctaa aaaattcctt gccgggacta ctaggcgggc
     3121 cgcgattctg tgcatttgtg ttgcaccgca tgtcgaggtc cgcactttac ccttcccgca
     3181 tgttcagcct gtggataaaa tttactgacg atctccaaat tcacatgttg gctcgttaat
     3241 tgctctcccc gcaccctcag caatcctggt tcttaacagc tatggaagtt atcatcggcc
     3301 tggcgcgatc cgactacgaa aaactgttgc acgacatttc ccctcaacat tccgcttacg
     3361 ctgttcttcg ccgttctccg gagcttgacc gttgggccga tgaaaggccc ttctcaatgt
     3421 gtgtcgtcat tgagtgcagt cgtcaggaag cgctggagct ctatcaagcc gcgaagcagc
     3481 actgccccca tatcctacgt gacatagaat acggccttaa ctcgtcccgc ggtacgtaac
     3541 cagaaagaga gtcggaatga aatcaattct tttgtcggct gccatcatca tggctataac
     3601 gcgcttatgc cgaaatcgag atcctgaacg caaaggtatg cgacctgaaa ccttgccgat
     3661 ttgtttttcc caacagagtg ttgaaaacct gaacaaggat ccacggcgac cttatgatct
     3721 tttcaacttc tattatcctc ccggccaaac aagcgtccgc tttcactaaa gcccgtttcc
     3781 tccgttcaaa ccgtccagtc ggcatttatt atttcctttc cactgccggc gtttcgctga
     3841 ttcagactgc gatgaaacgt tcagcgtgaa ccgctcgaca gccgtaaatc tcaagagaag
     3901 tttgtcgcaa cacgctgatt gagttggcag ctatgacccg gcgctaaccg actttttcct
     3961 cccggcgcgt aagccttagg ccggtgtcaa aaactccaaa ctgtgaggct caacggttat
     4021 atcaattaca gttagagacg gcggaaaatt cgcgccgttt tcgggccatg ctcgatcgtc
     4081 gatgtgaatc ggcgtccctt cccattcgat atgtatctgc tttcctcttc gaacgggcaa
     4141 gccggtaggc gctcggctgc cttccttgca ctcctcgaga caacgatcaa gtgtatcgtg
     4201 cgcgcctcgg gttagacaca ccacgtcgaa cagaccatcg ccgggatcgg cgtcaggcgc
     4261 tagaaacaaa ttgggaccaa tcgattttat gttcatcacc tcgagtaaaa tatattcgcc
     4321 cgagagatca cggccgtcca cggtaacgtg gagttctatc tctggacacg tcaacagccg
     4381 cgcttgcaac atttccagaa ccgaggtgat tttctcttca ggctctgtca gatgagcaag
     4441 gtcaacattt cctcgagcat cgagccgggt catcatgtca gtgaacaacc cgatgcccag
     4501 cccctcgacg aaaggcgccg agccccaggg tccattggcc acgccggagt ctaatttctt
     4561 acgccctgca gcgctccacc cgtcgatcaa ctgcgccagt gagtaatcca tcaggttgag
     4621 tgtcttggcg atgttattgg ctgtccccag tggcaaaacc gcgatcggta ttcctctccc
     4681 taccatgatc tcggcaactg ttccgactat gccgtcgccg cccgctaccg cgacgagatc
     4741 cgccggttta tccaaagccc tttgccaatg gctgtccttg gacgattggt aaacaagctg
     4801 gtggccggca cgacgaatca gttcgaccgg ttcttcgccg catgttgcat cgtcgctgcc
     4861 ggcatccgga ttatgaatga gcgtaacttt catgactcct cttaccttgc tgaagcgcgc
     4921 aaatgtgaaa tactcatatt aacactgctg aagacgtttg taagttgcag ctcataaaag
     4981 gaggtttggt ccatgcgtat cctggttgcc aacgacgacg gcatttacag tccgggtatc
     5041 gccgttttgg ccgaagtggc aagcaagttc ggcgaagtgc gtgtagtggc gccggacgtg
     5101 gagcgttctt ctgccggaca ttcaatcacc gctatgcgac cgctttccta caggcgcact
     5161 ccgatcggca aatttgaagc ttaccgggtc gacggcacac cggcggactg cgttgcactc
     5221 ggaattttcc attgggaaaa agttgacgtg gtcctttcgg gtatcaatct cggcagcaat
     5281 ctgggcaatt ccatttggca ctccggaact ttagccgcgg ctaaacaggc gacgcttttg
     5341 ggtttgcgcg gtatcgcctt gagctcgact gtcaccatcg atgagccgaa cttcgacctt
     5401 ttgaaaccgt ctctcgagga ggtgttgacc cttctcttaa gcgataacaa ggatttctgt
     5461 ttggtcaatg taaactttcc agatgaaaag ccgaagggac tccgctggac ccgccagtcg
     5521 gtgcgccatt acgatggtaa aattgtaacc ggtgaggacc ctttaggccg caagcatttc
     5581 tggttcagtg tcgttccggt cgaagaaacg gaagagggga ccgatcgctg ggcagtcgag
     5641 caaggttatg tctcgctgac ccccttacgg ctggacctga ctaacgaaaa ggaactggcc
     5701 gcggctttgg cgcgctatcc gctggcgtga tcttcgctta gcgcgagccg ttgtccttca
     5761 ttgacggcgc cgcctcactc ctgtcagttc ccaatcggag gtagcagccg gccgtcagca
     5821 ctcaagggag ctagcatgcc tgatcaaccg ctcaatggaa gttgtctctg caaagcggtg
     5881 cgctatcaag tgacaccgcc ctttatgaga ttttctcact gttactgctc acgctgcagg
     5941 aaagcgaccg gaggagtgag agctaccaac attgcggtgc ccatcgacca gttccggtgg
     6001 acccggggag aggagctgat cgcgcgttac gacctgcctg aagccaagag cttcgcgcgg
     6061 caaatctgta cccgctgcaa ctgccccgtg ccccatgcgt cgcgagatcg ccagcgggtc
     6121 atcgttccag cgggtacttt ggacgatgaa ctcacgacca aacccacatc ccatggtcat
     6181 tggtcgagcc gcgtgagctg ggccgagatc gacgaatctg atttgccctg ttcggattga
     6241 aagctcctcg cttgagcagc ttatcacaat cggctcacca cgtgtcgccc cacgagttga
     6301 cagcctccac agtcgacctt agattttttt cgctggacac gaatctagcc caggaggttt
     6361 ttatggaact tacggtatgg cgcccgtttg ccgggctaga gagtttcgac tctcaaatca
     6421 accaactatt tgatcaggtt ctgggtcaag cctggatcgg ctcgagatcc gctagcaccg
     6481 ggggatatcc ggcggtggac gttttagaaa gtaaggattc gtacctgcta cgcgccgagc
     6541 ttcccggtat gaacaaggaa gattttaacc tagaggtgaa gaacggcatg ctgaccatca
     6601 gcggcgagcg tagacccgaa gagttatcta gcggcgtccg gtatcgcagt gttgagcgcg
     6661 tcgccgggag gttctcacga tcgttctatc tgcctgaaac cgttcaacag gatggaatcc
     6721 aggcgactta tcgcgacggc atcttggaaa tttacgtgcc taagctagag catgccaaag
     6781 cgcggcagat cagagtgagc tgactgctgg agtactggag taaatgacat aagggttgaa
     6841 tgtccgggtc gatcgcgcat ccgcggcaaa tctgctggtg tagctcgtaa cgccgggatt
     6901 gaactcggag cacaatccgg tacctaaaat acatgacgaa gattctaggg acgataacct
     6961 gttttttgct cttgggcgtt ctgcttgcgg tggctttgcc tatcaagcat ccgatcttag
     7021 ctgtgcaact aacttttgcc gaaccgccgg cgagtttgcc tgttccggtg gacggtgttc
     7081 gcccgtcagc gattcgtaac agctggcggg ctccgagaga tggcgggcgt tatcatcaag
     7141 gcatcgatat ttttgccaaa ccaggaaccc cggtgcgctc gactaccgag ggtatcgtca
     7201 tcagactggg gcaaaaccgg ttaggcggta atgtagtctg gataatggga ccggggcgac
     7261 aaaagcacta ctatgcgcat ctgagtcact ttggcgcgtt cgaaatcggc gaccgtgtct
     7321 tggccgggga tattatcggc tatgtcggtg ataccggcaa cgccctagga acgccgcatc
     7381 atctacacta tggcgtctac gaactcggcg gcagcgcgat caatccgttt cccctgctta
     7441 cagcaagggc caatcaggag cgagcttagc ggccagagct aacatcccca ggttcgcgcg
     7501 acatcgaatg tacccgctag gctgacgaac taacccgccg ccaagcgctg cactatgcat
     7561 ttgttaactc atctatatca tgtgataaag aactatgccc ttttctaagc ttggactttc
     7621 gcctaaggtt gtcgacggcg tccgcgccgc cggctacacc gatcccacac ccatacaact
     7681 gcgtgcgata ccgatcctga tggaaggacg cgatctcatc ggctcggctc aaaccggcac
     7741 gggaaagacc gccgcctttg cgctgccgat aatatcgcgc ttagggcaac ggcgcgccct
     7801 gcgcgcactt atcctagagc cgactcgcga actggcagcg caggtggaga ccgcgattcg
     7861 tgattacggc cgctttacca atctccgaac cgtcgttttg tacggtggca cgggttacgg
     7921 caaacaggac cagacactgc gtcgcggcgc cgatatcgtc gtggcaacgc caggccgttt
     7981 gcttgaccag attagccgcg gcatgctgcg cctcgatcaa atagaaatcc tggtcctcga
     8041 cgaagccgat cgaatgttgg acatgggatt catgcccgac gtgcgcaaga tagtggaaaa
     8101 atgcccccgt aaccggcaaa caatgttgtt ctcggcgacg gttccgccgg agatcgacca
     8161 gctctgcaaa tgggcattga ataaaccgga aaaaatcgaa atcggccagc gtcgctctcc
     8221 cgcggcgact gtaacccacg cgttgtatcc ggtggactcc gaacagaaac aggaactttt
     8281 ggaagaactg ctccggcgca ccgactacga tcaagtcctg attttctgtc gcactaagaa
     8341 cggcgccgat cgggtagtgc gcaagctcca tcaacaggga catgcggtcg cagttcttca
     8401 ttcccgccga actcaacgcg agcgcgagca cgccttggat ggctttcgca acggccgtta
     8461 cgaggtgatg gtcgctaccg atatagccgc gcgcggtatc gatgtggcgc aaatcagcca
     8521 cgttatcaac ttcgatgtgc cgcatcatcc cgaagattac gtgcaccgga tcgggcgcac
     8581 cggtcgcgcg cagagtgtcg gtgatgcttt tacgatcatg accggggaag acgtccagga
     8641 gattgccgct atcgaacgct tcatcggcca gaagatcccg cgggtgaagt tggagaactt
     8701 caagtactcg tatacacgac ttctcgatcc caatcccaag cccatttttg gccgcggccg
     8761 cggtcagggc gcgccgggta agcgttcgtt cggctacggc agacgaagat aaacgcagcg
     8821 ccgggctgtg tcgagcgcaa atcgagattt gcttccgcat cgtccaatca ggaatcaagt
     8881 ttgacttgct gtaagacaaa gtttcacggt ccagagactt tagggtggct ctcgcttctt
     8941 ctgggtatcc gcaacattcc tggtgcccgc aagccgatgt ccatcacagc aacggccctt
     9001 ggagatgcca atgagcagta caattgattt tgagcgactg gttcaggccg ccggcgacgc
     9061 catcatcgcc gccgatccgg aaggtaggat cgtttcatgg aatcccgcag cagaacggat
     9121 ttttggtttt acagcgcagg aagcgctggg acaaacgctt gatctgatta tcccggagcg
     9181 gtttcgcgcg cgtcattggg acggttacaa gcaagtgatg cgcaccggcc aaaccagata
     9241 tggtacggag attttgcgcg ttccggcccg tcacagagat ggccggcctt tatcgatcgc
     9301 cttcacggtg gcgctcttgt cccgcgcaga cggacagacg gaggcgatcg ctgctattgt
     9361 tcgcgacgag acctcccgct ggaatgaaga acgctcattg cgcgagcgac tgcgcaacct
     9421 tgaggagcag caatctaagt gatttttcac taagctccat ggtgtctgtg agttgcgcag
     9481 ttaggctcct gaactcgagc tggcgttgat gaacgtcgtg tgggaagaac tagcagccgg
     9541 atttcccgac gcggaaacga tggtccgcat aactatccgg ctgattgttg cgatgatttt
     9601 tggcgcggtg atcgggattc agcgcgaacg ggctggcaaa ccggcgggac tccggactca
     9661 catgctggtt tcttctggtg cggcggtttt tcttatcgcc tgcggagagt tcggcatgga
     9721 cccggacagc atttcacgtg tcatacaggg tttggtcacc ggaattggct ttcttggcgc
     9781 cggtgcgatc ctgaagctct atgagagacg ggccgtcgag ggtcttacca cggctgcggg
     9841 catatggatg acggctgcat tgggagtggc ggtgggtttg ggtcgtttcg gactggctcg
     9901 gatggcaacg cttctggcgt ggatgacgct ctcgctggtg cgccagctgg aacacatgtt
     9961 gaacagagga atccggaagt ccgaggaaga cacgatttcg gccgaagact agcagggtgc
    10021 tgaaaaagcc tcttgcaggc tgctcaaaaa gatctagagg cgagacgcgc gaaaaatcga
    10081 cgagcggagg cgtacttacg gtacgtcgca gcgaggcgat tgagcggcaa cgaagcatat
    10141 gggtcttttt cagcagcctg ctaggagctc catcaaggat taccagctca cccctcgccg
    10201 cacggaccag tcttaataag atccaccgtc cggcaagatc gcatctatga ttcacgattt
    10261 tgctgctcaa agatgaacta agacttcgtc gttttctcca gcaatagaca ctatcgatgg
    10321 attggctaga tgcacttcag tggccggcaa tggtcgtgac ggttagcgcc gcctggctca
    10381 ttggctcgca gcgaaagatt cggcgcatga tcggcttttg gtgcttcttg gtgagcaatg
    10441 tgctttggat catctggggc tggcacgctc gagcttgggc aatggtaata ctccagataa
    10501 tccaaagcgc attgctcaac aagaagcacc aaaagccgat catgcgccga atctttcgct
    10561 gcgagccaat gagccaggcg gcgctaaccg tcacgaccat tgccggccac tgaagtgcat
    10621 ctagccaatc catcgatagt gtctattgct
//
LOCUS       DASUTG010000883         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1618340, whole genome shotgun sequence.
ACCESSION   DASUTG010000883 DASUTG010000000
VERSION     DASUTG010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618340"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF089_20605"
     CDS             complement(<1..183)
                     /locus_tag="VF089_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176416.1"
                     /translation="MPKTAPPDLRDKISSVFTRIEDVTYVGLGVLLAVSALVLLVAAA
                     WSLIEGLSAAGPAKELT"
     gene            complement(184..576)
                     /locus_tag="VF089_20610"
     CDS             complement(184..576)
                     /locus_tag="VF089_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion fhipep protein"
                     /protein_id="HEX6176417.1"
                     /translation="MSQSTLRPAEVCKALLATLEASEGRRRRRKRDQTPDGIGLTFKR
                     ELLEQAVKDDPEPEAFEEWLLNYSASHESAGGIFAMARAVFEEWRLAHSMGEFKSWLE
                     QGAPSDDATEDSSFLSRDQQREFAKGRL"
     gene            complement(573..1448)
                     /locus_tag="VF089_20615"
     CDS             complement(573..1448)
                     /locus_tag="VF089_20615"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX6176418.1"
                     /translation="MTVNEFEALAREYPDWQPWLLIVEAVMREATDRSWEAWIPATEL
                     QATDVPRLAGAELTPDLSKIRRWQQHLLQIAAQSGAPKMSQLNSLQKVTMNWAAIFEA
                     ALCEDAAALKQMADELGVDPDPFSAVHALIAVPFLHACNRRWEGSRGPGWTTGYCPTC
                     GAWPAFAETRGIERSRYLRCGRCGAEWQAHALFCCFCGNSDHKELGSLLPEKSGSTRA
                     IDTCNRCRGYLKTFTKLQGSAATKLMLDDLASVDLDIAALEQGFKRPAGPGYALRSKV
                     VLKPGLGQRILGWTA"
     gene            complement(1445..1672)
                     /locus_tag="VF089_20620"
     CDS             complement(1445..1672)
                     /locus_tag="VF089_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176419.1"
                     /translation="MKLALLTIIAAVVVGCTSPETTRSRGGGPGADLGNRGSIVRMHE
                     GSRPYEDTPKIIGAEHPPLESARQASEFSRR"
     gene            complement(1674..>2392)
                     /gene="nrfD"
                     /locus_tag="VF089_20625"
     CDS             complement(1674..>2392)
                     /gene="nrfD"
                     /locus_tag="VF089_20625"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6176420.1"
                     /translation="IISGILLTVDLHRPERFWHMLLESNTFQPMFKYWSPMSLGSWAL
                     LIFGLFSFLSFLAALADSGRINWSGVRRLRPPGTIGGIISIVGGLFAFYVAGYTGVLL
                     AVTNRPIWSDSPLLGMLFVVSAASISAALMILLAERNRWTMPGIADLHRMDVWILALE
                     LIVLVAFLFTLGPVFRAWFSVWGVVLLLGVVVLGMLVPLFLSWRRDWLGGLNYTASAA
                     LVLIGGFILRMAIVLGAEAV"
BASE COUNT          560 a          716 c          663 g          453 t
ORIGIN      
        1 ggtgagctct tttgcaggac cggccgctga aagtccctcg attaacgacc atgcggcggc
       61 aacaagaagc actaacgcgc tgaccgcgag caaaacgccg aggccgacgt atgtcacatc
      121 ctcaatccgg gtgaagacgc tggatatttt gtcgcgcagg tctggcggtg cggtttttgg
      181 caactagaga cgtccttttg cgaattcgcg ttgttgatct cgggaaagga aggaagagtc
      241 ttcagtggca tcgtccgagg gcgcgccctg ttcgagccac gatttgaatt cacccatgga
      301 atgagccagg cgccattctt caaaaaccgc tctggccatc gcgaagatcc cgcccgcgga
      361 ctcgtgacta gcgctgtaat tgagcagcca ttcttcgaaa gcttccggtt ccgggtcgtc
      421 tttgactgct tgttcaagca gctcgcgctt aaaggtcaag ccgataccgt ccggggtttg
      481 gtcgcgtttg cgtcggcgcc tccgtccttc cgaggcctcc agcgtcgcca ggagagcttt
      541 gcagacctcg gcaggacgaa gggtcgattg actcatgcgg tccaacccaa tatccgctgg
      601 cccaaacccg gcttgagtac tactttacta cgcaatgcgt aacccggtcc ggccggccgt
      661 ttgaagcctt gctccaaagc cgcgatatct aaatcgacgc ttgcgagatc gtcgagcatg
      721 agcttggtgg cagcgctacc ttgcaacttg gtgaatgtct tgagatagcc gcgacaccgg
      781 ttacaggtat cgatggcgcg ggtggagccg gacttttccg gcaggagcga tccaagttct
      841 ttatgatcgc tattgccgca aaagcaacaa aatagcgcat gcgcctgcca ctccgcgccg
      901 caacggccgc agcgcaaata gcggctccgt tctataccgc gggtttcggc aaaggccggc
      961 caagcgccgc acgtgggaca atagccagtc gtccaccctg gtcctcgcga tccttcccag
     1021 cgccggttgc aggcgtgcag aaaaggcacg gcgatcaagg catgaacggc ggagaaaggg
     1081 tcgggatcga cgcctagctc atcagccatt tgcttgagcg ccgcggcatc ttcacacaag
     1141 gccgcttcaa aaattgccgc ccaattcatg gtgactttct gcaaggaatt gagctgagac
     1201 atcttaggcg cgccgctttg cgcggcgatt tgtagaaggt gctgttgcca gcggcggatt
     1261 ttactgagat ccggtgttaa ctcggctccg gctaatcgtg gcacatcggt agcctgcaat
     1321 tcggttgcag gaatccacgc ttcccagctt cgatccgtcg cctcgcgcat gactgcttcg
     1381 acgatcagaa gccacggctg ccaatctggg tactcgcgcg caagagcctc gaactcgttt
     1441 acggtcatcg acggctgaac tcgctggcct ggcgcgcgga ttctagaggc ggatgctccg
     1501 cgccaataat cttgggcgta tcctcgtaag gacgcgaccc ttcgtgcatg cggacaatag
     1561 agccccggtt gcccaggtcg gcgcccggcc cgccgccgcg gctacgcgtc gtttcggggc
     1621 ttgtacaacc gacaacgact gcggcaatga tcgttaacaa cgctagtttc atgtcatacc
     1681 gcctcggcgc ccaatacgat agccatgcgc aggatgaaac cgccgatcag cactaacgct
     1741 gcgcttgccg tgtaattaag cccgccgagc cagtcgcgac gccatgacag aaataatggc
     1801 acaagcattc ccagcacaac aacgcccagc aaaagcacca cgccccagac gctgaaccac
     1861 gcgcggaaga ccgggcccaa ggtaaacaag aaagcgacta agacgatgag ctccagagca
     1921 aggatccaaa cgtccattcg atgtagatcc gcgatgccgg gcatagtcca gcggttacgt
     1981 tcggcaagca atatcattaa cgccgccgaa atcgatgccg ccgaaaccac aaacagcatc
     2041 cccaaaagcg gcgaatccga ccagatcgga cgattagtaa ccgccagcag aactccggta
     2101 tagcctgcaa cataaaaagc aaagagcccg ccgacaatcg aaatgatacc gccgatggtc
     2161 cccggcggcc gtagcctgcg cactccggac cagtttattc gcccgctatc agcaagcgca
     2221 gcgaggaacg ataaaaagga aaatagtccg aagatcaaaa gcgcccatga acccaacgac
     2281 atcggcgacc aatatttgaa catcggctgg aaggtattgg attcgaggag catgtgccag
     2341 aagcgctccg gcctgtgcag atcgaccgtc aaaagaatcc cactgataat ca
//
LOCUS       DASUTG010000884         3114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1618466, whole genome shotgun sequence.
ACCESSION   DASUTG010000884 DASUTG010000000
VERSION     DASUTG010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618466"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF089_20630"
     CDS             <1..194
                     /locus_tag="VF089_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX6176421.1"
                     /translation="LDHFFHSNQFRLVELRRLPYFGSDHFPVYIKLNYEPDAEKEQEE
                     LHADQSQKEEAEEKIDAAV"
     gene            281..760
                     /locus_tag="VF089_20635"
     CDS             281..760
                     /locus_tag="VF089_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6176422.1"
                     /translation="MDIRYLESSDFDFVSEVVDEWWGGRPVRGLLPRLFFEHFRPSSF
                     ALERNGAIEGFLVGFRSQSSPTVAYIHFVGVSPSCRGRGYGRMLYGRFFEAVAGMGCQ
                     EVRCITSPLNNGSIAFHRRLGFEILPGNGKLDDVPVIVDYAGKGQHRVLFRKKLTNS"
     gene            complement(893..1201)
                     /locus_tag="VF089_20640"
     CDS             complement(893..1201)
                     /locus_tag="VF089_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176423.1"
                     /translation="MAEKETAIITVTCPCCSATLTIDPVLNAVLNHKMPPKAQLVEHL
                     KDAVEYVKAEATKRDEKYQQIASAEKNKSKVLEAKFQELFKKAKEEPITKPIKDIDLD
                     "
     gene            complement(1344..1712)
                     /locus_tag="VF089_20645"
     CDS             complement(1344..1712)
                     /locus_tag="VF089_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176424.1"
                     /translation="MFKRIDHVAIHVKDLRRSIEFYQKHFGFKHYFQQVATGGMQIAY
                     LKLGDTVLELTHKSDGAMSGFHFCLETDNFDQSVEALQKSGVELVRPPHDTAAREARE
                     EGWRRVVFAGPDGEQIELRG"
     gene            2013..2699
                     /gene="aat"
                     /locus_tag="VF089_20650"
     CDS             2013..2699
                     /gene="aat"
                     /locus_tag="VF089_20650"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX6176425.1"
                     /translation="MPIFRLVDEPIFPPPDYADPSGLLAVGGDLSSERLLEAYRLGIF
                     PWYSDDQPILWWSPDPRFVLQLEDFHISRSLRKTLRQGIFQVTIDRAFDEVIAACAAV
                     PRIGQNGTWITSEMQESYSNLHGLGYAHSVETWFEGKLAGGIYGVSLGKAFFGESMFH
                     RKSDASKVALAALVEKLKSWSFHFLDAQMTTEHMMSLGAKEVSRRIFLKQLHVALEYP
                     TRRGKWRIKD"
     gene            complement(2708..>3114)
                     /locus_tag="VF089_20655"
     CDS             complement(2708..>3114)
                     /locus_tag="VF089_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176426.1"
                     /translation="GFSYAAPARAVFWLLTLVDIGYYIYWKRHLTPEAVLRNASASKR
                     LRALEGYKGGEEERAAAVVSTYVTRKVVLFAIIEAIAVYGMILALVGRFLLDQYLLSA
                     LALVLLIVEFPSEKSLSTVLQKLEHNTATQQN"
BASE COUNT          697 a          805 c          837 g          775 t
ORIGIN      
        1 cattggatca cttcttccat tcgaatcagt ttcgcttggt ggaactccga cggctgccgt
       61 acttcggttc agatcatttc ccagtttata tcaagctaaa ttacgagccg gatgctgaga
      121 aggagcagga agaattgcac gcggatcagt ctcagaaaga agaggccgag gagaagatcg
      181 acgccgccgt ctgaatggct aaagaaatca cgcgtcgacg gagtccgcca gtagttcacc
      241 gctgacgggc tagcttcaaa aaaatcaatt tcgcacgctg atggatatcc gttacttaga
      301 atcctccgac tttgactttg tgtccgaggt tgtcgatgaa tggtggggag gtcgccctgt
      361 gcgcggcttg ctgccgcgtc tcttcttcga gcactttcgc cctagcagct tcgcgcttga
      421 gaggaacggc gcgatcgagg gatttctggt gggctttcga tcacagtcat cacctaccgt
      481 cgcgtacatt cattttgtcg gtgtttcacc gtcttgccgc ggacggggtt atgggcggat
      541 gctttacggg cgcttttttg aagcagtcgc cggcatgggc tgccaggaag tccgctgcat
      601 cacgtcgccg ctcaataatg gctcgattgc ttttcatcga cgcctgggct ttgaaatcct
      661 tcctggtaac ggaaaactag acgacgttcc ggttattgtt gactatgccg gcaaaggcca
      721 gcatcgagtg ctcttccgca aaaagttaac aaattcttga ctcccctccg gcacaaattc
      781 caactgctct gctctcaaga gcgaatcaga aagctgatga gagtaaacca gtagcgctag
      841 ctttggccgg tggccggcct aagagatgaa aatattccgg atttagatcg gattagtcca
      901 gatcgatatc ttttattggc ttggtgatcg gctcttcttt tgccttcttg aacaactctt
      961 gaaacttggc ttccagaacc ttggatttat ttttttcggc agaagcaatc tgttggtact
     1021 tttcgtctcg cttggtggct tcagccttga cgtattccac ggcatccttc agatgttcta
     1081 ccaactgcgc tttcggcggc attttatggt tgagaacggc attaagaact ggatcgatcg
     1141 tcaatgtcgc gctacaacaa ggacaggtca cagttatgat tgcggtctct ttctcagcca
     1201 ttatcagccc tccaatctcg catcattatg caaaagcact ccagcggcga ttttggattt
     1261 tccgactgcc tattttcggt tgaattcgtg actccgaacc caatctccca atgttcaatc
     1321 cgaaatccaa aatctaacat gcgtcatccg cgcagttcga tctgctcccc gtcgggtccg
     1381 gcgaaaacga cccggcgcca gccttcttct cgcgcttccc gagcggcagt atcatggggc
     1441 gggcgaacta attcgacgcc gcttttctgc aacgcttcga cgctctgatc aaagttgtcg
     1501 gtttccaagc aaaagtgaaa cccgctcatg gcgccatcgg acttatgggt cagctctaac
     1561 acggtatcgc ccaatttcag gtaggcgatc tgcatccctc ctgtggcgac ctgctggaaa
     1621 tagtgcttga agccgaagtg cttctggtag aattcgatgg agcggcgaag gtctttgacg
     1681 tggatcgcta catggtcgat gcgcttgaac atactgtgct ccttaggcga aatgataggc
     1741 tcggagtggg acggtttgca agcgaatgaa aaggcgacag agtaagctta agcttggtta
     1801 agccgctgtt gcgaatcccg cttttcctga ctgttatgaa gcccagggac acgagtaatg
     1861 tgtagtgacc tttcaatgta ggctgctcaa aagatctcag aaggcgaggc gcgccaagtt
     1921 cggcgagcgg aggcgtacgt ttgagtacgt tggagcgagc cgggcaagcc cagcatatga
     1981 ggtttttgca gcaggcttac caggctaatc cgatgcccat ctttcgactg gtagacgaac
     2041 cgatttttcc gccccccgat tatgccgatc ctagcggact gcttgcggtc ggcggcgatc
     2101 tatcgagtga gaggctgctg gaggcttatc gcctgggcat tttcccctgg tattccgacg
     2161 atcagccgat cctctggtgg tcgccggacc cgcgttttgt gctgcagctg gaagacttcc
     2221 acatctcaag gagcttaaga aagacgctgc gccagggtat tttccaggtt acgatagacc
     2281 gcgccttcga tgaagtcatc gcagcttgcg ctgctgtgcc gaggataggc cagaacggca
     2341 cctggatcac ttcggagatg caggaatctt atagcaatct ccatggtctg gggtatgccc
     2401 attcggtaga gacctggttc gaaggcaaac tggccggcgg aatctacggc gtttctctcg
     2461 gtaaagcatt cttcggcgaa tcgatgtttc accgcaagag cgatgcctcg aaggtggcgt
     2521 tagccgctct ggtcgaaaag ttgaaaagct ggagttttca ttttctcgat gcgcagatga
     2581 ccaccgagca catgatgagt ctgggcgcga aagaggtttc ccggcgcatt tttctaaagc
     2641 agcttcatgt ggcattggag tatccgacca gacgtggcaa atggcgcatc aaagactagc
     2701 cggataatca gttttgctgg gtggctgtat tgtgctcgag cttctgaagg actgtcgaga
     2761 gagacttctc cgagggaaat tccacgatca aaagaaccag cgccaaggcc gatagtagat
     2821 attgatcgag caagaagcgg cccaccaatg ctaggatcat gccgtagacc gcaattgctt
     2881 cgataatggc aaagagcacg accttgcggg taacgtaagt cgaaaccacc gccgccgcgc
     2941 gttcttcctc gccgcctttg tatccttcca aggcccgcag tcgtttgctc gcactagcat
     3001 tgcgcagcac tgcttccggc gtcagatgac gtttccaata aatgtagtag ccgatatcaa
     3061 ccagcgtgag aagccaaaac acggctctgg caggggccgc ataggaaaac ccgc
//
LOCUS       DASUTG010000885         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1471862, whole genome shotgun sequence.
ACCESSION   DASUTG010000885 DASUTG010000000
VERSION     DASUTG010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471862"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VF089_20660"
     CDS             complement(<1..1022)
                     /locus_tag="VF089_20660"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HEX6176427.1"
                     /translation="MTTDDGIKFVDTTIRDGHQSLWAENMTTGMMLPVAERMDRAGFD
                     AIELISSSHLKKCVRELKEDPWARVKLMSQRITRTPLRLNAGRFSAFDITPKSMYRLF
                     MERMAANGIREARISEEWNELEGWRWKTEVARDVGINPVLNLIYSVSPKHTDQYFAQR
                     TRDAASLKVNRLCLKDPGGLLTPERVKTLVPIIFENADGIPVELHTHCTTGLGPICCV
                     EAVKLGIKIVNTALPPLSDGSSNPSLFNVAKNLRARGYQTLIDEELLKPVSEHFTHIA
                     KREGFPIGEPLEYDYTQYQHQVPGGMISNLRHQLRKVGLEHKIDQALEETMQVRAELG
                     YPIMVTP"
     gene            complement(1121..1978)
                     /locus_tag="VF089_20665"
     CDS             complement(1121..1978)
                     /locus_tag="VF089_20665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176428.1"
                     /translation="MARDIVAIDVHIHLSDETTQKAKGSRTAQMAKYFGRERPPVSID
                     ELADQYRARKMMAVLMNTRDETVTGLTPVPNDHVASAVKKHPDVFLAFGAIDPWTGKL
                     ALDEIRRCKDLGLHGMGELNPARQHFFANDVRFYPLWEECAKLNMPILFHGGMAAAGA
                     GTPGGMGIKLKYNQPIYLDEVAADFPELKIISAHPTWPWTAESLAIARHKANYFIDLS
                     GWAPKYFPAELLHNINTLLQDKAMFGSDWPAIGVERWLEEFQQVNMKPEVRKKIMLDN
                     AVKFFGLTL"
BASE COUNT          468 a          610 c          612 g          531 t
ORIGIN      
        1 ggcgtgacca tgattggata gcccagctcg gcgcgcactt gcatcgtttc ttcaagcgcc
       61 tggtcgatct tgtgctccaa tccgaccttg cgcagctgat ggcgcagatt ggagatcatt
      121 cctcccggca cctggtgctg gtattgggtg tagtcatact ccagcggctc gccgatcgga
      181 aacccttcgc gcttggcgat gtgggtgaaa tgttccgaga caggttttaa cagctcttca
      241 tcgatgagcg tctgataacc ccgcgcgcgg agatttttag cgacattgaa aagcgaaggg
      301 ttcgatgacc cgtcggacaa cggcggcaag gctgtattga cgatcttgat gccgagcttg
      361 acggcttcca cacagcagat cggccccaat ccggtggtgc agtgcgtgtg caactcgacg
      421 ggaatgccat cggcattttc aaaaataatc ggcaccagag tttttacccg ttcgggggtg
      481 agcaagccgc cgggatcttt taggcacagc cggtttactt tgagcgaagc cgcgtctcgg
      541 gttcgttgcg cgaaatactg atccgtgtgc ttgggcgaaa ccgaatagat cagattgaga
      601 accgggttga taccgacgtc gcgcgcgact tcagttttcc agcgccatcc ttccagctcg
      661 ttccactctt cggaaatccg tgcctcgcga ataccgttag cggccatgcg ttccatgaac
      721 agccggtaca tcgacttggg cgtaatgtcg aaggcgctaa accgccctgc gttcaagcgc
      781 agcggcgtgc gggtaatccg ctggctcatc aacttgaccc gagcccaggg atcttctttg
      841 agctcgcgca cgcatttctt caaatgggaa ctggagatca attcgatggc gtcgaagccg
      901 gcccggtcca tgcgctctgc cacaggcagc atcatgcccg tggtcatgtt ttccgcccac
      961 aggctctgat ggccgtcgcg gatggtggta tcgacgaatt tgatgccgtc gtcagtggtc
     1021 atcgccgtat gaaatggagt aatgaagcga tggaatgctt atttggatta cccgaacccc
     1081 agcactccaa tccttcagta ctccatcgct ccaatctgtt ctaaagagtg agaccgaaga
     1141 acttcaccgc gttgtcgagc ataatcttct tgcgcacctc gggtttcatg ttgacctgct
     1201 ggaattcttc caaccagcgt tcgacgccga tggccggcca atctgagccg aacatcgcct
     1261 tgtcctgaag cagggtattg atgttatgca gcagctcggc agggaaatac ttgggcgccc
     1321 agccggatag gtcgatgaaa taattggctt tgtggcgcgc gatggctaag ctctccgccg
     1381 tccagggcca ggtcggatgg gcgctgatga tcttcaactc cggaaaatca gcggccactt
     1441 cgtccagata aatcggctgg ttgtatttca atttaatgcc catgccgccc ggcgtgccgg
     1501 ctcccgccgc cgccatgccg ccgtgaaaca gaatcggcat gttgagcttg gcgcattcct
     1561 cccagagcgg ataaaagcga acgtcgttgg caaaaaagtg ttgccgcgcc ggattgagct
     1621 cgcccatccc gtgcaaaccc agatctttac agcgacgaat ttcatccaag gcgagcttgc
     1681 cggtccaggg atcgatggcg ccgaaggcga ggaacacgtc aggatgtttc ttaaccgcgc
     1741 tggcaacatg atcgttgggt accggggtta aaccggtgac ggtttcatct cgcgtgttca
     1801 tcagaaccgc catcattttg cgcgcccgat actgatccgc caactcgtcg atggaaaccg
     1861 gcggtcgttc ccggccaaag tattttgcca tttgcgcggt gcgcgagcct ttagcctttt
     1921 gtgtggtttc atcggataga tgaatgtgaa cgtcgatagc gacgatgtct ctagccataa
     1981 tctaagctcc tccttgtttg tctcgacagc cggttagaat ttttaaatat catttcttat
     2041 tgcactgctg aaaacttagt tcaaggcggc cacaaggacg agctcgtttt caagatcgat
     2101 gttcagagca aatcttgcct cctattgcac gagctggtga tgttgagctc tgaacctcga
     2161 accggcgaac gaaagtccgc cgcttatgcg cgcttctcaa tcgtcataga aaacgcgggt
     2221 g
//
LOCUS       DASUTG010000886         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1472093, whole genome shotgun sequence.
ACCESSION   DASUTG010000886 DASUTG010000000
VERSION     DASUTG010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472093"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..314
                     /locus_tag="VF089_20670"
     tRNA            240..314
                     /locus_tag="VF089_20670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Gly,seq:ccc)
     gene            complement(826..3477)
                     /locus_tag="VF089_20675"
     CDS             complement(826..3477)
                     /locus_tag="VF089_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX6176429.1"
                     /translation="MTQEELRLQEDRNRKANWKRWGPYLSERQWGTVREDYSPYGTAW
                     NSFSHDQARSRAYRWGEDGLAGICDKHQFICFALALWNGRDPIIKERLFGLTGSEGNH
                     GEDVKEYYFYLDSTPTHSYMKYLYKYPQSEFPYARLVEENRRRGKLDLEFELISTGVF
                     DQDRYFDVFVEYAKGSPDDVLIRIEAINRGPEAAELHLLPTVWFRNTWSWNAEPPPPR
                     LMRGEPAEGVIPIELHHSYYNKRWFYCEGNPETLFTDNETNHRRLFGADNASSYAKDG
                     INDYIVSGAKDAVNAAGVGTKAAGHYRLNIGPGKSAVVKLRFTDKEMVSSTSRPFGAS
                     FDLTVAERKKEADEFYASVSPPSLSADSRSVMRQALAGMLWSKQFYHYVVKQWLDGDP
                     GNPPPPAERVNGRNHEWPHLYNADVISMPDKWEYPWYAAWDLAFHCIPLALVDPDFAK
                     EQLCLMLREWYMHPNGQIPAYEWAFGDVNPPVHAWAAWRVYKVEKKRRGKGDREFLQR
                     IFHKLLLNFTWWVNRKDANGMNIFQGGFLGLDNIGVFDRSAPLPTGGYIEQSDGTSWM
                     AMYTLNLLAIALELSKEDRAYEDVASKFWEHFIHIAHAMSHRGQNGTGLWNDEDGFFY
                     DVLRLPDGSHFPMKIRSMVGLIPLFAVETLEPELLEHLPGFKRRLEWFIENRPDLTGN
                     VACMRTPGMGERRLLSIATQDQLRQMLKYMLDEREFLSPYGIRALSQIHRDYPYRLSV
                     NSMDHRVDYEPAESSTGLFGGNSNWRGPIWFPVNFLLIESLQKYHFYLGDSFKVECPT
                     GSGKMMTLWEVAGEISRRLMNIFLQGADQRRPVFGGTEKFQNDPHWRNLIPFHEYFHG
                     DNGAGIGASHQTGWTGLVAKLIQQYQG"
BASE COUNT          801 a         1052 c         1000 g          889 t
ORIGIN      
        1 atcgtaccta agccaacagc gctcacccgc aagcctgttc taccaagaat tcggtatttc
       61 attcatcctc ctacgtcgcg ccaacataaa acacgtctgc gctgctgcgc aagatcacga
      121 acgatgttgg agaaacccgc cagcgctggg acggctgcag ttgctgctag cacggcacta
      181 actctcattg acaagctccg ggtaaagcca tattttaact ccacatccac cctgcttgag
      241 cgggcgtaat tcagcggtag aatgccagct tcccaagctg gacgtcgccg gttcgacccc
      301 ggtcgcccgc tccaaacttt tcaaaggctt acacgtttcc gtgtaggctt tttttatttc
      361 cgggaatttc gggttttctt ttcgcggaaa atatttcttc tcaaaatgtt ttccaactgc
      421 tgaacttgtt agttggagga ctgcaatgtc tcgtcgatga ttgcgaagct gtgacaggaa
      481 agtactcgag gatctatttg cagatttgcc cattcttgtg gaagtgcgac gcaacgttga
      541 atcccgaagt ctcgattgcg gcgacgatct tacgatataa gaagtgcaag aatgtagagg
      601 tgatgccgag ctgtggccgt cttcacagtt aagctttctg ccggatctaa attgcttatc
      661 agtttacgcg gaaagattca gagctttttc gaaatctctt ctaacagaac tttatctctc
      721 aaccatacct gctgtttcag caattacagg aagatcctaa tcgataaact ttcgcgcccg
      781 taacccgttt cgtcgccaag ttggtttgtt ccccaggcca atgcctcaac cctggtattg
      841 ctggatcagc ttagccacta gaccggtcca accggtttga tggctagcgc ctatgccggc
      901 tccgttatcg ccgtggaaat attcgtggaa cggaatcaga ttgcgccagt gcgggtcgtt
      961 ctgaaacttt tccgtgccac cgaaaactgg tcttctctga tctgcaccct gcaagaagat
     1021 gttcatcagc cgccgcgaaa tttccccggc aacctcccag agcgtcatca tcttgccgga
     1081 gccggtgggg cattccacct tgaaactgtc tccaaggtaa aagtgatatt tctgcaacga
     1141 ttcgattagc aggaagttta ccgggaacca gatcgggccg cgccagttgg agttgccgcc
     1201 gaagagtccg gtgctggatt ctgccggctc gtagtcgacg cggtggtcca tgctgtttac
     1261 cgacaaacga tagggataat cgcgatgaat ctgcgagagc gctcggatgc cgtacggcga
     1321 aaggaactca cgctcgtcga gcatatattt gagcatttgc cggagctggt cctgggtcgc
     1381 aatcgaaagc agtcggcgct cacccatccc cggcgttctc atgcaggcaa cgttgccggt
     1441 taagtccgga cgattttcta tgaaccactc cagccgccgc ttgaacccag gcaagtgctc
     1501 cagcagctcc ggctccaagg tttcgacggc gaacaacgga atcagtccga ccatggaacg
     1561 aatcttcatc ggaaagtgac tgccatccgg cagcctgagc acgtcataga aaaagccgtc
     1621 ttcgtcgttc catagtcccg tgccgttctg accgcgatgg ctcatcgcat gcgcaatgtg
     1681 aatgaaatgc tcccagaatt tgctcgccac gtcttcgtag gcccggtctt ccttggatag
     1741 ctcgagcgca atggccagga ggttcaaagt gtacatcgcc atccagctcg tgccgtcaga
     1801 ttgttcgatg tagccgccgg tgggcagcgg cgcgctgcga tcgaatacgc cgatgttatc
     1861 cagccctaaa aatcctccct gaaagatgtt cataccgttg gcgtccttgc ggttcaccca
     1921 ccaggtaaag ttcagcagca atttgtgaaa gatccgctgg agaaactcgc ggtcgccttt
     1981 gcctcgacgc ttcttctcga ccttgtagac ccgccatgcc gcccaggcgt gaaccggcgg
     2041 attaacgtcg ccaaaggccc actcgtacgc gggaatctgc ccgtttggat gcatgtacca
     2101 ctcgcgcagc atcaaacaga gttgctcttt ggcgaagtcc ggatcgacca atgcaagcgg
     2161 aatgcagtgg aaggccaggt cccaggccgc ataccacggg tattcccact tatcgggcat
     2221 cgaaatcacg tcggcattgt acagatgggg ccattcatga ttgcggccgt tgacccgctc
     2281 tgccggcggc ggagggttgc cggggtcgcc gtccaaccat tgcttgacca cgtaatgata
     2341 gaactgcttc gaccagagca tgccggcgag ggcctgacgc atcacgcttc gtgaatccgc
     2401 cgaaagactc ggcggcgaga cgctggcata gaactcatcg gcttcttttt tccgctccgc
     2461 gacagtgaga tcgaagctcg cgccgaaagg ccggctcgtg gaggatacca tttctttgtc
     2521 cgtgaaccgg agcttgacga cggcgctctt tcccggcccg atattgagcc gataatggcc
     2581 ggccgctttc gttcccaccc cggctgcatt caccgcgtct ttcgcgccgc ttacgatgta
     2641 atcgttgatt ccatccttgg cgtaggatga agcgttatcc gcgccgaaca gacggcgatg
     2701 gttggtctcg ttgtcggtga aaagagtctc cggattccct tcacagtaaa accatcgttt
     2761 attgtaatag gagtgatgca gctctatggg aatgacgcct tcggccggct caccccgcat
     2821 tagccggggc ggcggcggct cggcgttcca agaccaggta ttgcgaaacc acaccgtcgg
     2881 cagcagatga agttcggcgg cttcgggtcc gcgattgatt gcttcgattc gaattagaac
     2941 gtcgtccggc gacccctttg cgtattcgac gaaaacgtcg aagtaacggt cctgatcaaa
     3001 gacgccggta cttatgagct cgaattccaa gtcgagtttt ccccgccgtc gattctcctc
     3061 caccagacgt gcgtagggaa actcgctctg cgggtatttg tacaggtatt tcatgtaaga
     3121 gtgcgtcggc gtcgagtcca ggtaaaagta gtactctttc acgtcctcgc cgtggttgcc
     3181 ctcgctgccg gtcaggccga agagccgttc tttgattatt ggatcgcggc cgttccaaag
     3241 ggccaaagcg aagcagataa actgatgctt gtcacaaatc ccggcaagcc cgtcttcgcc
     3301 ccagcggtag gcgcggctcc gagcctgatc gtgagaaaaa gaattccacg ccgttccgta
     3361 cggactgtaa tcctcgcgca cggtgcccca ttgacgctcc gatagataag gtccccagcg
     3421 cttccagtta gccttacggt ttcgatcttc ctgcagtctg agttcttctt gtgtcacgat
     3481 gctctcctgt ttacggtgat cagcggcgaa ataaaacgct ggctggtcgt gccggattcg
     3541 ggatcgagag ttcgattacg gatcgtttcg ctgcggtgtt tacctgttag atgcagcttg
     3601 tgccggagag gatgcgcgaa attccggttt tttagagagt cgcagaacta ttcggtcaag
     3661 gcgaatcagc tgagctagcg agcaagctta aaatgcgcac ccgccggtgt gtgcggcgga
     3721 ccggtgaccg caaccagcgt at
//
LOCUS       DASUTG010000887         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1768918, whole genome shotgun sequence.
ACCESSION   DASUTG010000887 DASUTG010000000
VERSION     DASUTG010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768918"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VF089_20680"
     CDS             complement(<1..976)
                     /locus_tag="VF089_20680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176430.1"
                     /translation="MENKKADVSRRNFLKKSAALGVGAAVWPGLLHRASAASRDRVTI
                     FHSSVADSIHPYNHSSSPIYGNWQHVIEPLVELDYDKKEYFGILAESWEFQGKQWVFK
                     LRKGVKFHNGAPFTSKDVAFSIEKMRDEKGGSLQAPNFKDVTEVQTPDDQTVVFVTKQ
                     PLAIFLDRLENRFILSKVAGDKFGDQLYQNPIGTGPYKFVSYQRGGNMVFTRNDDYWG
                     GKAAIKEVVFRKVTEEAARLAALESGQADFINNVPVHEVARFQKHPRVRIDRLEGLRM
                     FLLAMNVAHKPFDNKLVRQAVNYSVDAPAIVKNIFDGIGYPLNGPVGSN"
     gene            complement(1003..1842)
                     /locus_tag="VF089_20685"
     CDS             complement(1003..1842)
                     /locus_tag="VF089_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176431.1"
                     /translation="MIGLKYAKIWVGGIILAALFVCGVASPLLAPHDPQAQTLEDRLR
                     PPSWAENGSPAYLLGTDNLGRDILSRIIYGSRISLLVGAATVIFAGLLGCTLGAIAGY
                     FGNVADDIVSKISEIFLAFPFLLIAIAIMAFLGQGVVNLIVALMLSRWVQYCRVVRGE
                     VLSIKERDFVTAVKALGGRDGYVLLRHVVPNTVASITVIATFAMAIVIISEASLSFLG
                     LGVPAHIPTWGSMLSEGRSYINRAPWLTIFPGLAIFITVFGINLLGDGLRDVFDPKLR
                     RQR"
     gene            complement(1842..>2471)
                     /locus_tag="VF089_20690"
     CDS             complement(1842..>2471)
                     /locus_tag="VF089_20690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176432.1"
                     /translation="NSLFDHAGMSIALIGVSLPNFWLGIMLIYFLGGQLNLLPVAGRI
                     EYGIEVKPITHLYLVDSLITGNLTAFWSALQHLLMPAVTLGTSLAAIVTRISRSSVLE
                     VMRQDFITTARAKGLNEKAVIWRHILRNAMITIVTILGLQLGALLNGSVITETVFSYP
                     GIGELLIQSISARDYKLTQVLILFFAVIYFVVNLLTDFLYSWIDPRIKF"
BASE COUNT          591 a          692 c          653 g          535 t
ORIGIN      
        1 cgttggaccc gaccggcccg ttgagcgggt aaccgatgcc gtcgaaaata ttcttgacga
       61 tggccggcgc atcgacggaa tagttgacgg cctggcgaac cagtttgttg tcaaacggct
      121 tatgcgcgac attcatagcc aacaaaaaca ttcgcaagcc ttccagcctg tcgattctta
      181 cacgggggtg tttttggaag cgggcaacct catgtaccgg cacgttattg atgaagtccg
      241 cctgaccgga ttccaacgcc gctaaccgcg ccgcttcttc cgtcactttg cggaaaacca
      301 cttctttgat cgctgccttg cctccccagt aatcgtcatt gcgggtaaag accatgttgc
      361 cgccgcgttg atagctgacg aatttataag gccccgtgcc gatcgggttt tgatagagct
      421 gatcgccgaa cttatcgccg gcgactttgc tgagaatgaa acggttctcc aagcgatcca
      481 agaaaatcgc caatggttgt ttggtcacga acacgacggt ttgatcgtcc ggtgtctgca
      541 cctctgtgac atccttgaag ttgggcgcct gcaagctgcc gcccttctca tctcgcatct
      601 tctcaatcga gaacgccacg tctttcgagg tgaagggcgc tccattgtgg aatttgaccc
      661 ctttgcgcag tttgaacacc cactgcttgc cttgaaattc ccatgactcg gcgaggatcc
      721 cgaagtactc tttcttgtcg taatcgagct ccaccagcgg ctcgatgacg tgctgccagt
      781 taccgtaaat cggactggag ctatgattat aaggatgaat cgagtcggcg acgctgctat
      841 gaaagatcgt gacccggtct ctggaagcgg cggacgctcg gtgcaagagt cccggccata
      901 ccgctgcccc gacacccaag gcggcgctct tcttcaaaaa gtttctacgg ctgacgtcgg
      961 cttttttatt ctccatttcg atcctcctag atcggactgt tgttatctct gtctgcgcaa
     1021 tttcgggtcg aagacatccc tcaaaccgtc tcctaaaaga ttgatgccga acacggtgat
     1081 aaaaatggcc aggcctggaa aaatcgtcaa ccatggagcg cgattgatat aactcctgcc
     1141 ctcgctcaac atcgagcccc aggttgggat atgcgccggc acgccgaggc cgaggaagct
     1201 cagactcgct tcggatataa taacgatcgc catggcgaag gttgcaatca ccgtaatgct
     1261 ggcaaccgtg ttcggcacca catgacgcaa cagaacataa ccgtctcgac cgcctagcgc
     1321 cttgaccgcg gtgacgaaat cgcgctcctt gatagacagg acctcgccgc gcacgacgcg
     1381 gcagtattgc acccagcggc taagcatgag cgccacgatg aggttgacca caccctgtcc
     1441 tagaaacgcc atgatggcta tcgcaattag cagaaacgga aaggcaagaa agatctctga
     1501 tatcttgctg acgatatcgt cggcaacgtt gccgaagtag ccggcgattg ctcccaaggt
     1561 gcaaccgagc aaacccgcaa agatgacggt ggcggcgccg accagtaagg agatgcgcga
     1621 tccgtagata attcggctca ggatgtcgcg gccgaggtta tccgtcccca gcaagtaggc
     1681 tggcgagccg ttctcggccc aagatggcgg ccgcagccga tcttcgagcg tttgtgcctg
     1741 tggatcgtgc ggcgccaaca gaggcgaggc tacaccgcaa acaaaaaggg ccgccaagat
     1801 gatgccgccg acccagatct ttgcatactt taatccgatc atcagaactt tatcctgggg
     1861 tcaatccagc tataaaggaa atcggtcagt aaattcacca cgaagtaaat aaccgcgaag
     1921 aacaagatca gcacctgggt tagtttgtaa tcccgcgctg agatcgattg gatcaataat
     1981 tcgccgatgc cgggatatga gaacacggtt tccgtgatca ccgatccgtt tagcagagcg
     2041 cctagctgga gaccgagaat ggtcacaatc gtgatcatcg cattgcgcaa tatgtgccgc
     2101 cagatgaccg ctttctcatt caaccctttt gccctggcgg tagtgatgaa atcctgccgc
     2161 atcacttcga ggacgctgga acgcgagatg cgagtaacaa tcgcggccag gctcgtgccc
     2221 agggtaacgg ccggcatcag caggtgctgc agagcgctcc aaaacgcggt caaatttccg
     2281 gtgatcaggc tgtcgactaa gtagaggtgc gtgatcggtt tcacctcgat gccgtactcg
     2341 atccgtccgg cgacgggcaa gagattgagt tgtcccccta gaaaatagat caacataatt
     2401 ccaagccaga aattcggcaa agaaacaccg atcagcgcga tgctcatccc ggcatgatca
     2461 aagagcgagt t
//
LOCUS       DASUTG010000888         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1472317, whole genome shotgun sequence.
ACCESSION   DASUTG010000888 DASUTG010000000
VERSION     DASUTG010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472317"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1219
                     /locus_tag="VF089_20695"
     CDS             155..1219
                     /locus_tag="VF089_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176433.1"
                     /translation="MRLRIKLSSLLGLLFGLGFLSFFVSPVYSQAPYYQGKTITILRG
                     GEPGGSGDMQARALIPFLRKYIPGNPSFIVENVPGAAGMKAVNQVYSSAKPDGLTIAA
                     VGSGLASGAILGLPGARYDIDKLIYLGSTEHGDPYVFLSRKEAGFDNLDKLRAAPSVR
                     IGAQTVGHAIYVSGRIFAYLLGLKEPRMVVGFGGRELDIALTRGEIDARANSADTVVR
                     RNAEALAKGEYNVHATLTIPKGKFHPRFADLPDADSLAKNDRERQLVDLFRAFLYPRW
                     PYVLPPGTPAATVKILQGAMVRAFKDPGFTAEFKKLMGGEPSPLSGEEVETSIRELPR
                     DPEVIGLYKKMAEHGPLPPR"
     gene            1833..3065
                     /locus_tag="VF089_20700"
     CDS             1833..3065
                     /locus_tag="VF089_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX6176434.1"
                     /translation="MVVATHGRVWQADYMLIGVPKESYPGERRVALTPAVLPSLIQTG
                     LKILVQAGAGAEAGHADEDYKKAGAEIIDSRQEIFSAPDVILQVLALNANPLAGLSDL
                     ALMRPHQALIAFLRPLGAQDNNITALAKAKVTGFAVEMIPRITRAQSMDALSSMSTVV
                     GYKAVLAAAEELPKMFPMLMTAAGTIKPARIFVIGAGIVGLQAIATARRLGAVVKAYD
                     VRPAVKQEVESLGARFVEIPLETGEEEKAGGYAKAKDETFYRRQQELLTGVIAESDVV
                     VTAAVVPGRKAPVLITEAMVEQMAAGSVIVDLAGEQGGNCALSSAGENVVRHGITIMS
                     PINIASTVPFHASQLYAKNLSNLLLYLVKDGQLRLERDDPIIKDVLITYDGEVVNERL
                     RDFLQLSPLEPGEHKQEA"
     gene            3065..>3231
                     /locus_tag="VF089_20705"
     CDS             3065..>3231
                     /locus_tag="VF089_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX6176435.1"
                     /translation="MEYLAAAVTIFVLAIFVGFEIITKVPPTLHTPLMSGSNAISGIT
                     LIGALLSAGTQ"
BASE COUNT          701 a          889 c          894 g          747 t
ORIGIN      
        1 tttacaagct ggatctcaga acatcgatga aagaattcga gaacgatcgc tttgcgaagc
       61 tcaaaaagga ttacgagctt gcaggcgcgc atcgaagcaa tttggcctat ggctatattg
      121 ccaaggcgta aacttaatgt cgggaggtca tcgcatgcga ctaaggataa aactctcttc
      181 acttctcggc ctactgttcg ggctcggttt cctgtcgttt ttcgtcagcc cggtttattc
      241 acaggcgccg tactatcaag gtaagacgat cacgatcctg cgcggcggcg aacctggcgg
      301 ttccggcgat atgcaggcac gcgcgttgat tccgtttctt agaaaatata ttccgggaaa
      361 tccgtcattt atcgtcgaaa acgtacccgg cgccgcaggc atgaaggcgg taaatcaggt
      421 ttattcaagc gctaagcccg atgggctcac gatcgctgca gtcggctcgg gtctcgcctc
      481 cggcgccatt ctgggcctcc ccggcgcccg gtacgatatc gacaagctga tttatttagg
      541 gtcgacggaa catggcgatc cctatgtttt tctcagccgt aaagaggccg ggttcgataa
      601 tttggataag ctgcgcgcag cgccttccgt gcgtatcggg gcgcagactg tgggccacgc
      661 aatctacgtg agcggacgca tctttgccta cctgctgggt ctgaaagagc ctagaatggt
      721 tgttggattc ggcggccgtg aactcgatat cgctctaacc cgcggtgaga ttgatgccag
      781 agcaaacagt gccgacaccg tggtgcgccg aaatgccgag gctctcgcca aaggcgaata
      841 caatgttcat gcgacactga cgattccgaa gggcaagttc catccacgtt ttgccgatct
      901 ccctgacgcc gactcgcttg caaagaatga ccgagagcgt cagttggtcg atctgttccg
      961 agcctttttg tatccgcgtt ggccctatgt gctgcctccg ggaacaccgg ctgccacggt
     1021 gaagatttta cagggagcga tggttcgcgc tttcaaggac ccaggtttca ccgccgaatt
     1081 caaaaagctc atgggcggcg aaccgtcgcc gttatccgga gaggaagtcg agacctcgat
     1141 tcgagaactg ccgcgcgatc cggaggtgat tggcttatat aaaaagatgg ccgagcacgg
     1201 tccgctgccg ccgcgatgaa gctcgaggat cggagcatag ccccctgact gaaaacatgt
     1261 aaatgagtct accggctcgt atcgcggctt tcgtcgcatg agcggcaaac agcaggttct
     1321 agagtgcaga tattgcgctc aaggtgatac caggcattgc gctacccaag tcaaagcgca
     1381 ggattttgtc acgttaatcc gccgcggacg ccgatactgt tgttcgtaac gccatccgct
     1441 tgaccgtcta ttccttagcc ttactttccc accacgacca cttctcttga tcgttctaat
     1501 tactcgacgc tgaaaccgat accgctgcac tcagcgactc ccgctatcga tcctgtctta
     1561 accgcaggtc cgctcgcttc gaaagattct ctgcgtaaac tcactctctc tgttcatcgt
     1621 cagggttata attccaacac tctacgtaat cactttggtg caacgcttgt cagcgaatcg
     1681 ataggatcca tggaaacgca tgaccctgcg cgttgaatga gcatgcctgg gggagagttc
     1741 gcaattcgcg cagctccttc tgttcttgtt ctgctcttgc aaagcatcat cagagtcact
     1801 gcttcgatgt cgatgcaaga ggtaaaagcg gcttggtcgt tgcgactcac ggccgggttt
     1861 ggcaggctga ctatatgctg atcggcgtac caaaagaatc ttatccgggt gaacgccgtg
     1921 tcgcgctcac gccagcggtt ttgccaagtc tgatccaaac cggcttgaag attttggtgc
     1981 aggcgggcgc tggcgcggag gcaggccacg ctgatgagga ttataagaag gccggcgcgg
     2041 aaatcatcga ctcacggcaa gagatatttt ctgcgcccga cgtcattctc caggttctgg
     2101 cgctgaacgc gaatccgctt gccggcttat cggatctggc tctgatgcga ccgcatcaag
     2161 cgctgatcgc tttcctccga cccctgggtg ctcaggacaa taacattact gccttggcga
     2221 aggctaaggt taccggattt gccgtcgaga tgattccgag aattacccgg gcgcaatcca
     2281 tggacgcgct ctcttcgatg tctacggtgg ttggctataa agccgttctt gccgccgccg
     2341 aggaattgcc aaagatgttt ccgatgctga tgactgccgc cggcacgatc aagccggcgc
     2401 gaatctttgt catcggcgcc ggtatcgtcg gtctgcaagc tattgccacc gcacggcgcc
     2461 tcggagcggt ggtaaaggcg tacgatgttc gccctgcggt aaagcaggag gtcgaaagct
     2521 tgggagcacg gttcgtggag attccgctgg aaacaggcga agaggaaaag gccggcggtt
     2581 atgccaaagc caaggacgaa acgttttacc gccgccagca ggaactatta accggagtca
     2641 tcgccgaaag cgacgtcgtc gttaccgcgg ctgtggttcc cggccgcaag gctccggttt
     2701 tgatcaccga ggcgatggta gaacaaatgg ctgcaggatc cgtgattgtc gatttggccg
     2761 gagagcaggg cggcaattgt gccctgagta gcgccggcga gaacgtcgtc cgtcacggca
     2821 tcacgatcat gtcgccgatc aatatcgcat ctacagtgcc ttttcacgcg agtcagctct
     2881 atgcaaaaaa tctttcgaac ttgcttcttt atctggtcaa agacgggcag ctccgactcg
     2941 agcgcgacga tccaatcatt aaggatgtcc tgattacgta tgacggcgaa gttgttaacg
     3001 aacgtctgcg tgacttcttg cagctctccc cgctcgagcc cggcgagcac aaacaggagg
     3061 catgatggaa tacttggccg cggccgtgac catattcgtg ttggcgatct ttgtcggttt
     3121 tgaaatcatc accaaggtcc cgcccaccct gcatacgcct ttgatgtcgg gctccaacgc
     3181 tatatcaggt atcaccttga tcggagctct cctgtcagcc ggcacccaac a
//
LOCUS       DASUTG010000889         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1174259, whole genome shotgun sequence.
ACCESSION   DASUTG010000889 DASUTG010000000
VERSION     DASUTG010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174259"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VF089_20710"
     CDS             <1..648
                     /locus_tag="VF089_20710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6176436.1"
                     /translation="RMRTTIVLADDHELVRESIASLLREVPNFEVVGQCANGRQLLAL
                     VENVRPDVAVVDVSMPELNGIEAARQLREISPRTRVIALSAYTDEAYIRDMIDAGVVA
                     YIIKSGAAKDLIQAIRTASRGRVYFSQEIMGGAHQVAYGRATCESAVRPLSQREREIL
                     QLVAEGKSSKEIAAILGISETTVKTHRNNIMGKLNVRDIAGLTRQAVRLKLVRID"
     gene            1252..2142
                     /locus_tag="VF089_20715"
     CDS             1252..2142
                     /locus_tag="VF089_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176437.1"
                     /translation="MMAHSSHTLRLKQGWTMLIVLCLSSLAGAPVGGSVNAPSADPIL
                     FDGSIAPGDVNDLGANDPDAGGQRLAEDFSFPDPVIVTSITFLGGYFPTSTPQTDSFT
                     LTIYKDNMGLPDPLSIVTQIALANLDRTDTGTDVFGIDLYTYTASFAGVSLLGGTRYW
                     LTIVNNTTGDTDDNWAWAGKHEVGNFARSFNGGVTWFDTPAGSFSFLLRGTVDECPNS
                     DLSTTVVIDGCNSGVPNTLFPSGCTISDRIAACAEGASTHGQFVRCVSDVTNDLKKAG
                     TITGQQGGAIESCAGRANIP"
BASE COUNT          754 a          875 c          862 g          792 t
ORIGIN      
        1 cgaatgcgca cgacgatcgt tttagctgat gaccacgaac tggtgcgcga gagcatcgcg
       61 tcgctgcttc gagaagtgcc gaatttcgag gttgtcggac aatgcgcaaa cggcagacaa
      121 ttgttggcgc tggtcgagaa cgtccgtcct gatgtggctg tcgttgacgt ctcaatgccg
      181 gagcttaatg gtatcgaagc agctcgccag ttgcgagaaa taagtccccg aacgcgagtt
      241 attgcgcttt ctgcctatac cgatgaagcg tacattcgag acatgatcga cgcaggcgtg
      301 gtcgcttata tcatcaaaag cggtgctgcc aaagacctca tccaggcaat ccgtactgcg
      361 tccagaggca gggtctactt cagccaggaa atcatgggcg gagcacatca ggtagcctat
      421 ggccgagcga cctgtgagtc tgcggtgcgc cctctgtcgc agcgcgaacg tgaaatcttg
      481 cagttggttg cggagggaaa atcatcgaag gaaatcgctg ccattttggg cattagcgaa
      541 accacggtca agactcaccg caacaacatt atgggcaaac tcaacgtgcg cgacatcgcc
      601 gggttgacgc gtcaagccgt cagactcaag ttggtccgta tcgattgaaa agtaatccaa
      661 agctgggcca caatgttggc agccgacagc aagaactatc cgtcccttgt caatcgtaac
      721 tgcttacgga ttcactgtca gattaacggg acaattcgcc accgagggtt ttccttcact
      781 cactgtccgc atcgacggct gtgcggaacg gtggtagtct ctagagacta tgagtaatac
      841 cacaataata attctcatca tcgttctact cctactcttc ggtggcggtg gctattttta
      901 ccgtcgacga tgagcagaac gccgcgagcc cgagcttttc gagggaagtg ctcacttacg
      961 gatgtattgg gagaaacgtt tgagtgcggc ggaacggcgt ttcactcgag cttgtgagac
     1021 tcttgctcgg gtcgcgaaaa ctctcacgca acacgccagc cttacagttc aacattgcaa
     1081 caatggaggc cagcaagtta acgtcgcgaa gtagccgatt acgttgactt cttaccgtca
     1141 acacggataa tctcaacgct tcgcaaacga aggctgagcc cgagcctctg cgtcctcgca
     1201 cactcgcttt attcagcctt gtcagttcct ctttcaatca gggagtcctc catgatggca
     1261 cattcatccc acacgcttcg actcaaacag ggatggacca tgctgatagt cctgtgtcta
     1321 agcagccttg caggcgcccc agtgggcggt tcggtcaatg ctcccagcgc tgatcctatt
     1381 ctttttgatg gaagcatcgc tccaggcgat gtgaatgacc ttggcgctaa tgacccagat
     1441 gctggtggtc aacggctggc ggaagatttc agctttcccg atccggtaat cgtcaccagc
     1501 atcacgtttc tcggcggtta ctttcccaca agcacgccgc agaccgattc gtttacactg
     1561 actatctata aggacaacat gggcctacca gatccgctgt ccatcgtaac gcagattgct
     1621 ctcgcgaatt tggaccgcac tgacacgggc acggatgtct ttgggatcga cctttacacg
     1681 tacacggcga gttttgccgg ggtgagtctc ctggggggca caagatactg gttgacgata
     1741 gttaacaata cgactggcga tacggacgat aactgggcgt gggccgggaa acatgaagtc
     1801 ggtaacttcg cgcggtcgtt caacggcggt gtgacgtggt tcgacacacc tgccgggagc
     1861 ttctcattcc ttctgagagg tacggtggac gagtgcccca attcggatct gagcaccacc
     1921 gttgtcattg atggttgcaa ttctggggtt cccaatacgt tgttcccaag cggctgcacg
     1981 atctctgacc gcattgcggc atgcgccgag ggtgccagca ctcatggtca gttcgtgcgt
     2041 tgtgtctccg acgtgactaa tgatctgaag aaagccggca ccatcacggg tcagcaaggt
     2101 ggcgcgatcg aaagctgtgc cggccgggct aacattccgt aacgtctgag cgagatgcga
     2161 gtacggaaat tctgaaaaac cgaaaagtcg atcatttctg atcggccttc cagccaagca
     2221 atcgagattg tgcaagagta ggctcactct ttctttgcga gcatctctcg tgctctttac
     2281 ccgtctgtgt gacggggtta aagacactga tcttgcatgt actcccgcta ctacactttg
     2341 tgtttgcttc aaccctccaa gcgccgtagc ctgtcctttc gcttcgaggc ggatgtgcct
     2401 ttagcgatga agcatccgct ttgagacatg gcccgcaatc gctttgatcg tttgctcgct
     2461 tgcgccactc tcgcatagtt ctgtaatcag gtgatgccgt agatcgtgaa agcgcaatca
     2521 gcgcaaaact gccttagttg tgagtggccg tcgaagccct ggcgcagggc cgtcacgtca
     2581 caagttacga cacagttgca cccgttaagg ccgctgattt cctgtaagtg attgattgta
     2641 ttggtaggcg cgtggggatt tgaaccgcag acccctaccg tgtcaacgcg acgctttacc
     2701 ctcacgaacc ccaataataa agggcctttt cgttcttttt cttttctttc cgaccaatta
     2761 cccgaccaat tactttgccg atggttaaga acttctagtc cttcaaaagg atttccctaa
     2821 gaaagaccag cacccacttt tgagctagag gggtaggtgc ttaacgatgt tctcccattc
     2881 actcatatcg ctgcacgaaa caattatttc aagctcattg atcacttggc tgatgggcaa
     2941 ttgcctcgaa acaacgatga caccagctac cttctctgat ctggcgatac gactggttgc
     3001 gtgataaggc attgtgcgtc gatcgtgagt aacgataacc cgttcgtgct cagctgccca
     3061 cgctaagagc tcgggatcgg atgcattact gagactgact tgatgagccg ttatgacatc
     3121 tacgttcgga acgcgtagta agagaccgcg aaggataaca tgatcgagat cttgatcgat
     3181 gagcaaccga agcatgaaag tgcggtctat gcggtcggcg ttgcctcgcc attctcttca
     3241 gccacggtcc accggcgcaa aattcgagag cgtagatcac caa
//
LOCUS       DASUTG010000890         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_435104, whole genome shotgun sequence.
ACCESSION   DASUTG010000890 DASUTG010000000
VERSION     DASUTG010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435104"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /locus_tag="VF089_20720"
     CDS             <1..1762
                     /locus_tag="VF089_20720"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX6176438.1"
                     /translation="LENANKELQEQLSALSSRLQSAEHEFAQLETEKRQLEVSNQKLN
                     DDAVALRQQLNSVTLANEEYHIERAAFDRQLASIGSELKSTTAKLEQAETRIEELENV
                     GVPSAPSSAERSLTESRTEFETSLKGLQRELADNVDKLQAFDSVKRQLLDLEQKYDEA
                     LLENRKLQQEITRWQERVPDNEAAQPDFGVIREMDDSPLQSDIADDQGQMQLDMVGLL
                     EKRSSSRQAMQPGVVLRSAASPGVSGMDGLESYGQTSAFQASIEYVEPTRDETNVPGV
                     DLELRPSPDSRTARLSHLLAWIRLHGSDTYREQIDSLKDFTDLSERERHVVRDIFLAR
                     ADEAQKRGRDDEMLRYRSWAKNIVYHTRFADDEIEDVSGIMTPENQHRYEASSDRTAM
                     DRVPATSVKAGLTTVQPEGIKSPAGNVALGIGVGLGLVLILLLQLFFDTSAEITVASD
                     DASPAASSRPVELPKRPQPIEENRSPIVSENTPDTEASRPVERVRAKSDDAKAAARNS
                     PKEPPAKTTRSQSLFGAHTIVKPTPVYSEPSEESALITSLDPGTQVNVVAARRGWYEI
                     RSRSGRPPGFIRQDGASRNR"
     gene            2027..2794
                     /locus_tag="VF089_20725"
     CDS             2027..2794
                     /locus_tag="VF089_20725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6176439.1"
                     /translation="MKLYSRIEGSGFPVIILHGLLGSSDNWRAISKRLSPSYKIYSVD
                     LRNHGQSPHSSKMTYPAMAEDVRELLEIEQVPDCHLVGHSLGGKVAMQFATSHPDLVS
                     KLVVVDIAPKGYPPSQRPVLAALEKLALKSFGAFGEIDAALAPAIPEVSVRQFLIKNI
                     ARVPSGGFQWRIDLATIVKSYDELTKPIIAAGSYDKPALFVRGGRSDYIAETDLPSIR
                     ATFTRAEFVTIPTAGHWVHAEASDEFLQILTAFLNKP"
     gene            2877..3257
                     /locus_tag="VF089_20730"
     CDS             2877..3257
                     /locus_tag="VF089_20730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6176440.1"
                     /translation="MAKRKSIHIDGMEHGAPIPNGVVIGNLVFSSAISGKDAKTGVLS
                     EDPVEQAHAMFRNLKLFMQKAGGTSDDIAHMKVYLKEEKYRDAVNKAWLEMFPDEHDR
                     PARHALKVELRGNVLFQIEVVAVL"
BASE COUNT          799 a          913 c          924 g          689 t
ORIGIN      
        1 actcgaaaat gccaataaag aattacagga acaactctcg gcgctatcga gcaggctcca
       61 gtcagctgag cacgagttcg ctcaattaga gaccgagaag cgccagttag aagtttccaa
      121 ccagaagctc aacgacgacg cagttgccct gcggcagcag ttgaattccg taaccctagc
      181 caacgaggaa tatcacatcg agagagcggc gttcgatcgg cagcttgcgt cgataggaag
      241 cgagttgaag tcgactactg caaaactcga acaagccgag acgagaatcg aagaattgga
      301 aaacgtcggc gttccgtcag ctccttccag cgccgagcgg tcccttaccg agtcgcgaac
      361 cgagttcgaa actagtctga aagggcttca gcgagaactc gctgataacg ttgacaagct
      421 gcaggcattc gattcggtca agcggcagtt gctcgacctc gaacaaaaat acgatgaggc
      481 gctgctcgag aatcgaaaac tgcaacagga aatcactcgc tggcaagagc gggttcctga
      541 taacgaggcc gcgcagccgg atttcggggt cattcgggag atggatgatt cacctttgca
      601 aagcgacata gcggatgacc aagggcaaat gcagttagac atggtcgggc tgctcgagaa
      661 gcgttccagc agccgccaag ctatgcaacc gggggtagtt cttcgaagcg cggcttcacc
      721 gggggtctcc ggaatggatg gcctagagag ttatggacag accagcgctt tccaggcttc
      781 aatcgagtat gttgaaccca cccgggatga aaccaacgtc cccggcgtcg atctggaact
      841 gcggccgtca ccggatagcc gaaccgcccg attgtctcac ctgctcgcct ggatacggct
      901 tcatggcagt gatacgtacc gagaacaaat cgactcgctc aaagatttta ccgatctttc
      961 ggagcgtgaa cggcatgtgg tccgggacat tttcctagcg cgcgccgacg aggcgcaaaa
     1021 gcgcgggcga gacgatgaaa tgctgcgtta ccgcagttgg gcgaagaaca tcgtctatca
     1081 cacccgcttt gctgatgatg agatcgaaga tgtatcgggg attatgactc cagaaaacca
     1141 acaccgctat gaggcaagca gcgatcgaac cgctatggat agagttcccg cgactagcgt
     1201 gaaagccggt ttgaccaccg ttcaaccgga agggatcaag agcccggcag gaaacgtcgc
     1261 gctaggtatt ggcgtaggac ttggattggt tctgattctt ttgcttcagc ttttctttga
     1321 tacatcggcc gaaatcactg tagcgtcgga tgacgcatcg ccagccgcca gctctcggcc
     1381 tgtcgagctt ccgaaaagac cgcaacccat cgaggagaac cgatcgccaa tcgtttccga
     1441 aaatacccct gacaccgagg cttcacgccc cgtagagcgg gtcagagcaa aatcagacga
     1501 tgctaaagct gcggctcgca actctcccaa agaaccgccg gccaagacca ctcgatcaca
     1561 gagtctcttt ggagcgcata cgatcgtcaa accgacgccg gtttacagcg aacccagtga
     1621 ggagtcggcg ctaattacca gccttgaccc cggcactcag gtcaacgtag tggcagcgcg
     1681 gcggggatgg tacgagattc gttctcggag tggccgtcca ccaggattca tccggcaaga
     1741 cggcgcctcg cgaaatcgtt gatcgacatc gcttagccgg acgcatcata ctggatgcgc
     1801 ccgagcgatc gagatctgtt gcactaaacg cgagaaccga accgccgatc caaactgccg
     1861 gcatttgtat ccattcatac tcgtgcgcta acagatggcg cgcgttagtc tccctgaaag
     1921 tccttccatc agtaccggcc cgcgcgcgcg cggcctttca catacccgca gcgcggctcc
     1981 ggctttcttt tagaagccat tcttgtcaga gtttactttt cccctcatga aactttactc
     2041 tcgcatcgaa ggatcaggtt tcccggttat cattctgcac ggtctcctcg gctcctcgga
     2101 taactggcgc gcgatcagca aacggcttag cccaagctat aagatctata gtgtagacct
     2161 gcgtaatcat ggacagtcgc cgcacagtag caaaatgacc tacccggcca tggccgagga
     2221 tgtccgcgag ctattggaga ttgaacaggt cccggactgt catctagtcg gtcattcgct
     2281 gggaggaaag gtcgcgatgc aattcgcgac tagccatccg gacctggtta gcaaactggt
     2341 agtcgttgat atcgcgccga aaggataccc gccatcgcag cgtccggtac tggcggcact
     2401 cgagaagctg gcgctgaaaa gttttggagc tttcggcgaa atcgatgcgg cgctcgcccc
     2461 tgcgattccc gaagtctccg tcaggcagtt tttgatcaaa aatattgccc gtgtgccatc
     2521 cggcggtttt cagtggcgca tcgatctagc gacaatcgtc aaaagctacg acgagctgac
     2581 caagccgatc atcgccgcag gttcctatga caaaccggct ctgttcgtgc gcggcggccg
     2641 atcggattac attgcagaga ctgacctgcc gtcgatcaga gcgaccttca cgcgggctga
     2701 atttgtcacg attcctactg cggggcactg ggttcacgcc gaggcttcgg atgaatttct
     2761 tcagatcctg actgcttttc taaacaaacc ctaatcgctg atagcctgcg gccggtagac
     2821 cgttgtccgg caccgtggta tgagacagct gaccatttgg agcgaggagg cctttcatgg
     2881 caaagcgtaa aagcattcac atcgatggca tggagcatgg cgcgccaatt cctaacggcg
     2941 tggtgatcgg caatctagtt ttctcttcgg cgatttccgg gaaggacgcc aaaaccggcg
     3001 tgctttccga agatccggtg gaacaagccc acgccatgtt tcgcaatctc aaattattca
     3061 tgcagaaggc cggcggcacg agcgatgata tcgcccatat gaaggtttat ctaaaagagg
     3121 aaaagtatcg tgatgccgtc aacaaggcgt ggctcgagat gtttcccgat gaacacgatc
     3181 gtcccgcgcg gcacgcgctc aaggtcgagt tgcgcggcaa cgtcttgttt cagatcgaag
     3241 tggtggctgt gctataagtt aggtagtcga tccggggcga aatatgacag cgaggaggct
     3301 ttgatgagtg tcgacgttat cgatc
//
LOCUS       DASUTG010000891         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_583253, whole genome shotgun sequence.
ACCESSION   DASUTG010000891 DASUTG010000000
VERSION     DASUTG010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583253"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF089_20735"
     CDS             <1..324
                     /locus_tag="VF089_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176441.1"
                     /translation="LIEQWTMTLDKLEVMRKLNEFDVPCGPILDMREIAEEKSLRDTG
                     TVVEVDHHKRGKFLTVGNPIKLSESPTEVKASPLLGEHTEEILKSIVGWSDAEIAAAR
                     NEGAI"
     gene            570..2690
                     /locus_tag="VF089_20740"
     CDS             570..2690
                     /locus_tag="VF089_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6176442.1"
                     /translation="MNNESLRATINSALAAGRRALTAPEAQQLCETYGISTPKQALAT
                     TASEAAQVAAKLGFPVVMKIVSDDILHKTEAGGVLVGIENAADVKAGFERLVRNAKQY
                     KSDASIQGVQVQQMLKGGQEVMVGAVTDVTFGKMIAFGLGGILVEVMKDITFRMAPVS
                     ETEALAMLDSIAAAEVLRGVRGGRGVNRAALADIISKVSKLVTDFPEINEVDLNPIFA
                     TDMGANAVDVRIVLGEPPRSRQRYSQSEILASMQRIMNPRSVAVIGASAEDGKIGNSV
                     MKNLINGGYQGAILPIHPKAGEILGRKAYPSVLKVDEEIDVAVFAIPAKFCAAAMEEV
                     GQKKIPGAVMIPSGFAEIGEQALQDELLAVARKHNVRVMGPNIYGFYYTPQNLCATFC
                     TPYDVKGKVALSSQSGGVGMSIIGFSRSTKMGVSAIVGLGNKSDLDEDDLLTYFEQDD
                     NTQVIAMHCEDLKDGRSFAEVASRVSKKKPVVVLKAGRTALGARAAASHTGALAGNDK
                     IYDDVLRQCGVVRAKSLNDMLQFARGLQVLPNPKGENVVIITGAGGSGVLLSDACVDN
                     GLSLMKMPPDLDAAFRKFIPPFGAAGNPVDITGGEPPTTYANTIRYALSEPRIHSLIL
                     GYWHTIITPPMVFAKLCAEVVQEARDKGIDKPVVASLVGDVQVEEASEYLYERGIVAY
                     PYTTELPVAVLGAKYKWARGAGVL"
BASE COUNT          641 a          765 c          831 g          572 t
ORIGIN      
        1 ctcatcgagc agtggacgat gactctcgat aaattggaag tcatgcgcaa gctcaacgaa
       61 ttcgacgtgc catgcgggcc gattctcgac atgcgcgaga tcgcggagga aaaatcgctg
      121 cgcgacaccg gcaccgtcgt cgaagtggat catcacaagc gcggcaaatt tctcacagtg
      181 ggaaatccaa tcaagctgtc agaatcgccg accgaggtca aagcctcgcc gctgttgggc
      241 gagcacacgg aagaaatctt gaagtccatc gtcggctgga gcgatgcgga aatcgccgcg
      301 gcgcgcaacg aaggcgcgat ttaatcttgc taagcactga atgcttcaca cggatgcacg
      361 agcctacgaa tcgacccggc agacatccgt cttattccga gatcaagccc tgaagtgacg
      421 agttcacctg ccatatgagc accgggaagg gtttattggt ttgctacttg gcaggttcga
      481 ttgaagtaaa cagcacagct ttagaacacg tggaattctc gttcctgtcc tcactcacta
      541 ttaaatcgac atgagcttcg gaggtttcga tgaacaatga aagcctgcgc gccaccatca
      601 actccgctct ggctgcgggg cgtcgtgcct tgaccgcgcc cgaagcgcag caactctgcg
      661 agacatacgg tatctctacg ccaaagcaag cgctggcaac gacggcgtca gaagcggcgc
      721 aagtagcggc aaagttgggt ttcccggtcg taatgaaaat cgtttcggac gatatccttc
      781 ataaaaccga agccggcggc gtgctcgtcg ggatagagaa cgccgcggat gttaaagccg
      841 gcttcgaacg gctcgttaga aatgccaagc agtacaagag cgacgccagc atacagggtg
      901 tgcaggtgca gcaaatgctc aaaggcgggc aggaagtcat ggttggcgcc gtgactgatg
      961 tcaccttcgg caaaatgatc gccttcggtc ttggcggcat tctggtcgaa gtgatgaaag
     1021 atatcacctt tcggatggcg ccagtgagcg aaaccgaggc gctggcaatg ctcgattcca
     1081 ttgcagctgc tgaggttctg cgcggcgttc gcggcggtag aggcgttaac cgcgcggcgc
     1141 tggccgatat catctccaaa gtatcaaagc tggtgacgga ttttccggaa attaacgagg
     1201 ttgatttaaa cccgatcttt gccacggata tgggtgccaa cgcggtggac gtgcggattg
     1261 tcctgggaga gccgccacga tcccgccagc gctacagtca gagcgaaatt ctcgccagta
     1321 tgcagcgcat catgaacccc cgttcggttg ccgtgatcgg cgcctccgcg gaagacggca
     1381 agatcggtaa ttccgtgatg aaaaatctta tcaacggcgg ttaccaagga gcgatcttgc
     1441 caattcatcc gaaggccggc gaaatcctcg gacgcaaggc gtacccaagc gtgctcaaag
     1501 tggacgagga aatcgacgtt gcggtattcg cgattccagc caaattctgc gccgcagcga
     1561 tggaggaagt cgggcaaaag aaaattcccg gcgccgtgat gatcccgtcc ggcttcgctg
     1621 agatcggcga gcaggcgctg caagacgagt tgcttgcggt ggcacgcaag cacaacgtgc
     1681 gggtgatggg ccccaatatt tacggctttt actatacgcc gcaaaatctc tgcgccactt
     1741 tttgtacgcc ctacgacgtc aagggcaagg tcgcgcttag ctcgcagtca ggcggcgtcg
     1801 gtatgtcgat tatcggattt tcgcgctcca cgaagatggg cgtatcggca atcgtcggcc
     1861 tcggcaacaa gtcggacctc gacgaagacg atctcctgac ctatttcgaa caggatgaca
     1921 atacccaggt catagcgatg cactgtgaag atttaaaaga cggccgttcg ttcgctgagg
     1981 tggcgagccg ggtgtcgaaa aagaagccgg tggttgtcct caaagccggg cgcacggcgc
     2041 tgggcgcgcg cgccgcggcg tcccatacgg gggccctggc gggaaacgat aagatctatg
     2101 atgatgtgct gcgccaatgc ggcgtcgtcc gggcgaaaag tttaaatgac atgctgcaat
     2161 tcgctcgcgg cctgcaggtg ctgccgaatc ccaaagggga gaatgtcgtc atcattaccg
     2221 gtgccggcgg atcgggcgtg ttgttgtcag acgcatgcgt cgacaacggc ttgtccttga
     2281 tgaaaatgcc gccggacctc gacgctgcgt ttcgcaaatt cattcccccg tttggcgccg
     2341 ccggcaatcc cgttgacata actggcggcg agccgccgac aacctatgcg aatacgattc
     2401 gctatgcgct cagcgagccg cgcattcact cgctcattct cggctattgg cacacgatca
     2461 ttacgccgcc gatggtcttc gccaaactct gcgccgaagt tgtccaggag gcgcgcgata
     2521 aaggcatcga caaaccggtg gtcgcgtcgc tggtcggcga cgtgcaggtt gaagaagcga
     2581 gcgagtatct ctacgagcgc ggcattgtcg cttatccgta cacgacggaa ttaccagtcg
     2641 cggtgttagg cgccaagtac aaatgggcac ggggagccgg ggtgctgtag gcgatcaatt
     2701 gtcgctacgc tttcgttgca atcgttccag ctgttttcgg tcgtgtagcg cctctcctag
     2761 aaccgcaaac gagcctacgc tgtaaatttc tttaatgacg gcccataca
//
LOCUS       DASUTG010000892         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2066127, whole genome shotgun sequence.
ACCESSION   DASUTG010000892 DASUTG010000000
VERSION     DASUTG010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066127"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..837)
                     /locus_tag="VF089_20745"
     CDS             complement(145..837)
                     /locus_tag="VF089_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX6176443.1"
                     /translation="MIDLHYWTTPNGHKITIFLEEVKLPYSIKPVNISKGEQFAPEFL
                     KISPNNRIPAIVDHEPVDGKGPLSVFESGAILQYLAEKTGKFLPADVRGRTEVMQWLF
                     WQMGGLGPMAGQNGHFNNYAPEKIPYAIERYVKETERLYGVLDERLEGRDFIARDYSI
                     ADMACYPWIVPHERHKQKLDEFPNLKRWFSTIEQRPAVQRAYDLAKKINTQPTVNQDS
                     KSILFGQGRRRA"
     gene            complement(936..1706)
                     /locus_tag="VF089_20750"
     CDS             complement(936..1706)
                     /locus_tag="VF089_20750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176444.1"
                     /translation="MGKLRHIAFISQEPKRLSDFYQKHFGFEECKVFPSGSRMVIDPL
                     FNLAFLQSRGDQAGPVVGTHRADGAELERQPGIHHYGFLVENLNEAVAKLPASLNRGV
                     SPQVSAGVGGPEGARPAEMRFVDPWGNNVDLSARGFLGREEKKLPGVRLLAVQVPELA
                     AACEFYQRQFDLEITGWTDDGTVRLSDGTVTLLLTKTQVRPKSGVQYFGIQVDDLVTV
                     KRSLQNSGVTVSQEGAEMIQFLDPEGNRVVISQTGWTS"
     gene            2082..2801
                     /locus_tag="VF089_20755"
     CDS             2082..2801
                     /locus_tag="VF089_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX6176445.1"
                     /translation="MAEIKLTGKIARERIRMVEVPRPPQGIIEGFRALGDASGIVSDV
                     MDELGITGVIGASVLKPTIAGQSIVGPALTVRNIMQREHVYEAARRHVNKMAEFEAHN
                     LALPGDIVVIDGVAGISNMGGISAQTGKRQGEAGAIVFGGVRDVGHSHRVDYPVWATE
                     ITPVTGKWRIETIEINGEIVVGGVRVASGDIVLADDTGVCFIPRARAAEVLALAQRKS
                     AAEEAKCKAIDSGMAVADLPA"
     gene            complement(2928..>3310)
                     /locus_tag="VF089_20760"
     CDS             complement(2928..>3310)
                     /locus_tag="VF089_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176446.1"
                     /translation="AISFLLLVVVFASSYALLIPGAAGTANPDLFVFPKNQYGPADKV
                     WDEIMAGIAKAEADYVRAVLRRGGVAPAYKEQIIAFRDEAIYWDAVAKVMVYHSDFES
                     ARQAGKKAEILLRQMAGVIAGEKT"
BASE COUNT          696 a          979 c          900 g          735 t
ORIGIN      
        1 tgccgtcgaa tccagccgat ctctcttccg gtccattccc cctcgtacat cacctcgccg
       61 ccgtgacgcg agcaccgcgg ctgacccaag aagcctgcga tcgtgaacct cgagtcgcgt
      121 ttctctgtcg ctcgctgtat caagctacgc tctgcgccgg ccctggccga ataggatgga
      181 cttggaatcc tggtttaccg tcggttgggt attgatcttc tttgccaggt cgtaggctcg
      241 ctgcacagcg ggccgctgtt cgatggttga aaaccagcgt ttcaagttgg gaaactcatc
      301 gagcttctgc ttgtgccgtt catgcggcac gatccacgga tagcaggcca tatcggcgat
      361 cgaataatcc cgtgcgataa aatcgcggcc ctcgagccgc tcatcgagca cgccgtaaag
      421 gcgttcggtc tctttgacgt agcgctcgat tgcgtagggt atcttctccg gcgcgtagtt
      481 gttgaaatgg ccgttttgcc ccgccatcgg accaagcccg cccatctgcc aaaagagcca
      541 ctgcatgacc tcggtccgcc cgcgcacatc ggcaggcaag aacttacccg tcttctcagc
      601 cagatattga aggatcgcac cggactcgaa aacgctgaga ggtcctttgc catctaccgg
      661 ttcgtggtca acgatggccg gtatgcgatt gttcggcgag attttcagaa actccggcgc
      721 aaactgctcg cctttagaaa tattgaccgg cttgattgag tacggcagct tgacttcttc
      781 caggaagatt gtgattttgt gcccgttggg cgtcgtccag tagtggagat cgatcatcag
      841 tcactcccgt cctttttgtc acaactacac cgctggcgcg gaaagcgtca agcgagagta
      901 tgaatttcgg atctacacgc gctcattcaa gaagtctagc ttgtccagcc ggtctgcgat
      961 atcaccaccc gattcccctc cggatcaagg aactgaatca tttcggcccc ctcttgcgaa
     1021 actgtgacgc cgctgttttg taagctcctc ttcactgtca ccaaatcgtc cacttggata
     1081 ccaaagtatt gcacgccgct cttgggacgc acctgtgttt tggtcaagag cagggtcact
     1141 gtcccgtcac tcagccggac agtcccatca tcggtccaac cggtaatctc gaggtcaaac
     1201 tgccgttgat agaattcgca agccgcagcc aactccggaa cttgcaccgc gagcagtcgc
     1261 actcccggga gctttttctc ttcccttcct agaaagccgc gcgccgacag gtcgacattg
     1321 tttccccacg gatcgacaaa tcgcatttcc gccggccgcg ccccctcagg accgccgact
     1381 ccggccgata cttgcgggct caccccacga tttaacgagg ccggcagctt agcgacagct
     1441 tcgttcaagt tctctacgag aaagccgtaa tgatgaattc ccggttgccg ttccagctcc
     1501 gcaccgtcgg cgcggtgtgt gccgacgacc ggccccgcct gatcacccct gctctgcaag
     1561 aaggccagat tgaatagtgg atcaatcacc atgcgcgagc cgctgggaaa gactttgcat
     1621 tcctcgaagc cgaaatgctt ctggtaaaaa tcgctgagtc tctttggctc ctgggaaata
     1681 aacgcgatgt gtctgagctt acccatgttt cacctctcgc atctgtcaac tgtttatagg
     1741 ctggggattt attgcctgtc aaagcgatgg gcacagcgct ggcgagcgcg gtcgccgtct
     1801 ggttcgtcct gtgtggcgcc aggtagccat cacagttttg atcgctggct caggatgagc
     1861 ttctcattga gcaggattgg ttcaaggttt gaatcaagca atgaccttga gcaggtgctg
     1921 agaaatttac caagatggca tcacagccta gacgaatcgc tctcgacaac gaaacggcta
     1981 tcacatccta caaagcccga atcgatacgt ttttgtctcg cttgacggta agattctccc
     2041 gtcctatttt ggccctcata ctgctgcagt gaggtgaacg aatggctgag ataaaactta
     2101 ccggcaagat cgcgcgcgag cgcattcgaa tggtggaggt gccgcgaccg ccgcagggaa
     2161 ttatcgaagg gttccgggct ctcggtgatg ccagcggcat cgtctccgac gtgatggatg
     2221 agcttggcat taccggcgtc atcggcgcat cggtgctcaa accgaccata gccgggcagt
     2281 ccatcgttgg cccggcgctt accgtccgca acattatgca gcgcgagcat gtctacgaag
     2341 ccgcgcgacg gcatgtcaac aaaatggccg agttcgaggc gcacaatctc gctctaccgg
     2401 gcgatatcgt ggtgatcgac ggtgtggccg gaatctccaa tatgggcggc atctccgccc
     2461 agaccggcaa acgccagggt gaggcgggcg ctatcgtatt cggcggtgtg cgcgacgtcg
     2521 gccactcgca ccgggtcgac tatccagtgt gggccacgga gatcacgccg gtcaccggca
     2581 agtggcgcat cgaaaccatc gagatcaacg gcgaaattgt agtcggtggc gtacgcgtgg
     2641 catccggaga tatcgtgctc gccgacgata ccggcgtttg ctttattccg cgcgcccgcg
     2701 ccgccgaggt cctggcgcta gcgcaacgaa aatccgcagc cgaagaagct aagtgtaaag
     2761 ctatcgattc cggtatggcc gttgccgatt tgccggccta gtctccagag gagatcgcaa
     2821 actttggtta caggcagagg cgacggcgcg gcaccgagcg ctgatacgca acgttgctct
     2881 atggcaccaa accattgcgg ttatttgccg cgcctatgtg tgattcacta agtcttctct
     2941 cccgctataa cgcctgccat ctgcctgagg agtatttcag cctttttccc ggcctgacgg
     3001 gcagattcaa agtcggaatg gtagaccatg accttggcta ccgcatccca gtaaatggcc
     3061 tcgtcacgaa acgcgattat ctgctccttg tatgccgggg caacgccgcc acgacgtaga
     3121 accgcgcgca cgtagtcagc ctctgcttta gcgatcccgg ccataatctc atcccaaact
     3181 ttgtccgccg ggccgtactg gttcttcggg aagacgaaaa gatcaggatt ggcggtgcca
     3241 gcagcgccgg gaataagtaa cgcgtagctg gaggcaaaaa caaccaccaa caagagaaag
     3301 gatatagcaa
//
LOCUS       DASUTG010000893         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_583319, whole genome shotgun sequence.
ACCESSION   DASUTG010000893 DASUTG010000000
VERSION     DASUTG010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583319"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..927
                     /locus_tag="VF089_20765"
     CDS             190..927
                     /locus_tag="VF089_20765"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX6176447.1"
                     /translation="MKLAELHWPEVEKLDREKIVALIPMGSMEQHGPHLPFQVDVFCA
                     SRLAEDLEQKIPDVLLVPSLWAGVSAHHMDFPGSITLRARVFIDVLHDICGSLHHHGF
                     RRIVLLNGHGGNRSSLEVLGQELFVEFGLTVNTLAYWDLVPDLVKSLKKTKSSGMGHS
                     GELETSLMLYLAPRLVNRQDIPQGVLGIEPPGPTSGIKRYVNMKEHSAQGVIGMPSAA
                     SPETGKELYEGVLNALEKAVRALQADH"
     gene            complement(1193..2269)
                     /locus_tag="VF089_20770"
     CDS             complement(1193..2269)
                     /locus_tag="VF089_20770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176448.1"
                     /translation="MVKSKSFVLLSLFLWLSASTLLGALSHLEFVKADTAHAQSKPFY
                     EGKTVRVIVGPSGGYDYWARLLARYMPKYIAGNPSFVVQAMPGAGSVIATNYVYNLAQ
                     PDGLTVGMPTQQIYMGEFVGNDEVKFQMRKFLWIGSPDRNPAILYIRADTPYRTIDDV
                     VKSKVPPKCGGTGWESSSLIVALEEALGAKFELVLGYQGANEVDLAVVRGEVVCRDLG
                     LTAHFSREPFLSWHAKGFDRHLLQTGRKRDARAADTPTIFELMDRYKTPEVNRRAIEV
                     LTAGEGFGHPMMAPPGTPVDRVKILREAYAQALRDPELIAEAQKGRWVIEPVSGEELQ
                     SLAERIMVQPPDVVNQVKKILRVK"
     gene            complement(2294..2941)
                     /locus_tag="VF089_20775"
     CDS             complement(2294..2941)
                     /locus_tag="VF089_20775"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6176449.1"
                     /translation="MNSGINKVDVPAQHYDRLFIPVACVVSITTVDPAGRINAASFAT
                     CVRVHHEPTCIAFCVDSHKDTYRNVLATDEFVVNIPSFDREILEKVRTLGLPFAPGVN
                     ELEKAGLTALPSKVVKPPRIAEYKSHFECRVEWTKEWFGTRLMVVGRVVAASVDEDCV
                     DQDGWAILDKLRPAHYCGQAYSGDNKHRFVASYEIIEIDTVYDGPEASGKDPRRP"
BASE COUNT          782 a          994 c          921 g          822 t
ORIGIN      
        1 gccgagcgct tttcgtctat tccgccgtct acgacgagcc gctcgcgctg tcaaggcata
       61 ccttcacgtc tcagaagcaa tctcagttag gcgtcaacta aaggagctac cctgaatggt
      121 gatctcttgc tagggcgata gctgatttgg taacaatctc gaaacgattc tcacgggaag
      181 tgacgaagca tgaaactcgc tgaacttcac tggccggaag tggaaaagct cgacagagaa
      241 aagatagtcg ccctgattcc tatggggtcc atggagcaac acgggccaca cctgcccttt
      301 caggtcgatg ttttttgcgc gtcacggctt gccgaggatc tcgagcagaa aatcccggac
      361 gtcctgctgg tgccgtctct ttgggccggc gtttcggcgc accatatgga ttttccgggc
      421 tccatcactt tgcgcgccag agtcttcatc gacgttttac atgacatctg cggttcgcta
      481 catcatcatg gcttccgccg gatcgtgctg ttaaacggcc acggcggcaa tcgctctagc
      541 ttggaagtgc tgggacaaga actcttcgtc gagttcggct tgaccgtgaa cacattggct
      601 tattgggact tggtgccgga tctcgtcaag tcgctcaaga aaaccaaatc gagcggcatg
      661 ggtcattcgg gcgagctcga aacgtcgctg atgctttacc tcgcgcctcg gttggtcaat
      721 cggcaagata tcccgcaggg agttctcggc atcgaaccgc ccggcccaac gagcgggatc
      781 aaacgttacg tcaatatgaa ggagcattcg gcgcaaggtg taatcgggat gccgtcggcg
      841 gccagtccgg agaccggcaa ggagctatat gaaggcgtgc tcaacgcgct agaaaaagcg
      901 gttcgcgccc tccaggcaga ccattgattt gggcgcatcg ccatttcatt tcttgaaaaa
      961 ctttgcgtat ttttcaccga gctgcttgca atcgctctcg ctgcgctgga tctcttcgac
     1021 gaatttgatc ttatcggcgt ctttcaacag cggatgaact ccgcgtaaat tgacggaact
     1081 cgcaatcctt ggcgagcgct tccatgctct ctcggctgta ccaaaggtcg atccacaatt
     1141 tggccgacgc gattctttgg ccgaaatagc gccctgtttt tttgccggtg ttctatttga
     1201 cccttaaaat ctttttgacc tgattcacca catccggcgg ctgcaccatg atgcgctcgg
     1261 ccaagctttg taactcttca cccgaaaccg gttcgatcac ccatctgcct ttctgtgcct
     1321 cggcaatgag ctcaggatct cgcagcgctt gcgcgtatgc ctcgcggagt atctttactc
     1381 gatctacagg cgtaccgggg ggagccatca tcgggtgacc gaagccctct cccgcggtca
     1441 acacctcgat agctcggcgg ttgacctccg gagttttgta ccgatccatg agctcgaaga
     1501 tggtcggcgt atccgcggcg cgcgcatcgc gcttgcgccc agtctgcagc aggtgacgat
     1561 cgaaaccttt ggcatgccac gagagaaacg gttctctgct gaagtgcgct gtcaggccaa
     1621 ggtcccggca tacgacctca cccctgacta ccgccaagtc gacctcgttg gcgccttggt
     1681 aacccaacac caattcgaac ttcgcgccca aagcttcttc caaagcgaca atcaggctcg
     1741 agctttccca cccggtgcca ccgcacttgg gcggcacctt gcttttgacg acatcgtcaa
     1801 tggttctgta aggagtgtcg gccctgatat aaagaatcgc agggtttcga tcgggagagc
     1861 cgatccagag aaacttccgc atctgaaatt tcacttcatc attgccgacg aattcgccca
     1921 tgtagatttg ttgcgtaggc ataccgacgg tgagaccgtc gggctgtgcg agattgtaaa
     1981 cgtagttggt cgcgatgacc gatcccgcac ccggcatcgc ctgtacaacg aaacttgggt
     2041 tgccggcaat gtatttgggc atataccgcg cgagaaggcg cgcccaataa tcgtaacctc
     2101 cggaaggacc cacgatcact ctgactgttt taccttcgta gaaaggtttg ctctgagcat
     2161 gggcagtatc tgctttaaca aattccaagt gcgataaggc tcccaacaat gttgaggcgg
     2221 agagccaaag aaacaaagac agcaagacga atgacttcga tttcaccata tgccttccct
     2281 cgatgttaga acactacggc cgacgcgggt ctttaccgct agcttcgggt ccgtcgtaga
     2341 ccgtgtcgat ttcgataatt tcgtaggagg cgacaaagcg gtgtttattg tctccggagt
     2401 atgcctggcc gcagtaatga gccggtcgaa gcttgtctag aatcgcccaa ccatcttgat
     2461 cgacgcagtc ctcatcgacc gaggcagcga caactctccc gaccaccatc agccgggtgc
     2521 caaaccactc cttggtccat tcgacccggc attcaaaatg gcttttgtac tcggcgatgc
     2581 gcggcggctt taccaccttt gacggcaacg ccgtgaggcc ggctttttcg agctcattga
     2641 cgccgggcgc aaaaggtaac cccagcgtcc tgactttttc caagatctcg cgatcgaaag
     2701 acggaatatt gaccacgaac tcgtccgttg cgagaacgtt ccgataggta tccttatgcg
     2761 agtcaacgca gaacgcgatg caggtgggct cgtgatgaac tcttacgcag gtcgcaaaag
     2821 acgctgcgtt gattcggccc gccggatcga cggtcgtgat cgacaccaca cacgccaccg
     2881 ggataaacaa tctgtcatag tgctgcgccg gcacatcgac cttattaatg ccagagttca
     2941 agatcgcctc cagtttttaa ttgggcgatc ctatacaaat tcggcgtgaa ttacacgcgg
     3001 tggcggtgtc atgcagcgct gagacacgag aaaggcaacc agccacaact ccgttcgaat
     3061 cgtacaaacc cgtccatctt ttcgctcatg ccgggtccgg acagttcaag agcgtaatcg
     3121 tggcaggcgt gagttcttga ctaatttccc tctgcgacca cccactttga agttttcggc
     3181 gcggtttctt tcgcgcaata accgcggcaa gtaagtttga ggcttgaaca taattgagcc
     3241 gtagcgaata gcgtcaagat cctagcgacc ggccacaggt ctttaaatcg gactggccgc
     3301 gaatccgact tgccgcatga cgcgctcagg tttccgtttt ttaatcacgg tgcacgaatc
     3361 ccagccggat gaccttcgca atctactgga ttgctcgatg tgggggcaag aggcaccgcc
     3421 ggtgaaatcg acagcgagtc ttgatcttcg tacgctctgc caacatcctg cgaaacaacc
     3481 ggattgtgtt caatataaaa ggtatgaaag tttctatcg
//
LOCUS       DASUTG010000894         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2216110, whole genome shotgun sequence.
ACCESSION   DASUTG010000894 DASUTG010000000
VERSION     DASUTG010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216110"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..514)
                     /locus_tag="VF089_20780"
     CDS             complement(380..514)
                     /locus_tag="VF089_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176450.1"
                     /translation="MRMPDHQSTGVGVVRMRTITVGDPFGTQPELFRLLLAGPANDHI
                     "
     gene            complement(691..1545)
                     /locus_tag="VF089_20785"
     CDS             complement(691..1545)
                     /locus_tag="VF089_20785"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX6176451.1"
                     /translation="MLINVFYWIIIALVVYISPATLMAAQKERPIDVVEDNSFLLEEA
                     YNQEPGTVQHIFHAHYVNDTRRRGWEFNFTQEWPIFSQNHQFSYTIPSYHLVENGDRQ
                     NGLGDVLLSYRYQLLEEDETKPAFAPSFSLILPSGSRKRGTGHGVVGYEWGLPFSKKI
                     NGQFALHANFGLTYLPNARGRLASGGLSSEHSLVSYNLAASAIYAALHRVHLMLEWLG
                     DFEESIDDNGRSERSFKPVLSPGFRAAIVDQPMLQITAGAAAPIGLKRNAENYGAFLY
                     LSIEHDLF"
     gene            complement(1685..2701)
                     /locus_tag="VF089_20790"
     CDS             complement(1685..2701)
                     /locus_tag="VF089_20790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176452.1"
                     /translation="MRNPLKPGKIANALLIAAQLITAWILPVAHGAQPEKLNVCYSSI
                     AATSVSTWIPKEAGIYQKYGLDVNTIYVSGAQAISTLVSGDTHIVQGSGAAAVLSRLS
                     GADVTIIGTTINVVPMSLVTTPDIGRVEDLKGKTFGVSRFGSLTDIGLRKAVSEFGLD
                     PTTDIKMIQTGGVPEILLFMQQGVIKGGLISSPTLERAKELGYKELLNLGEIKYRYPG
                     TALVTTGGFIKNRPQTLNRFLKATLEGIKYAKANPDFTVRVLGKYTRNTDTKLLNSAF
                     KSYVLGYIRDVPTITQAEMESVLEDVATRNPKAKDADPKQFYDLAPLEQLIKEGFVKE
                     LYPG"
BASE COUNT          697 a          739 c          765 g          706 t
ORIGIN      
        1 gaaccgctgg agattcttcg ggtcacggcc aaagatcaaa aggtcgagaa tcaacgagtt
       61 gatcactcgg cacgaaagca atggatggtc gatcaaaata cgttcggtac ccggtaagga
      121 tatgaaagct gaccggataa cggcctgaat accgtcgtct ctagaacgca acaccaatcc
      181 caggtctctt tcttaccaat gggacacgca tcggagcttt tggttgtttt gtgtcattta
      241 cgataattta gcggattcgt cttttttggt atgtcggttg ctctgtttgg aaaccatgga
      301 attcatttgc caacattgcg acgaagtagc cacaggcgag aaatatcggg tgatcagcga
      361 ggaaaacggc cttgtatttc taaatatggt cgtttgccgg tcctgccagc agcaagcgaa
      421 acagctcggg ttgcgtgccg aacggatcac ctacagtgat cgttctcatt cgcaccactc
      481 ccactccggt ggattgatgg tcgggcattc gcattcatag ccctcgtttg ccgcttcatg
      541 tttgagtacc ccgacattcg ctcggggtat tcattcgtga gctcggctgc ggtcccttac
      601 aactcgagct ccagaaagca gcttgtatcg ttcatgctgc ggtcagagtc accgcaagcg
      661 tgttactcgg ctacacggac catgcgcctc tcagaacaga tcgtgctcga tcgagaggta
      721 gaggaaagca ccgtagtttt ccgcgttgcg cttgagaccg atcggcgcgg cagctccggc
      781 ggtaatctgc agcatgggtt gatcgacgat cgccgcgcgg aaacccggag acaagaccgg
      841 cttgaatgag cgttccgatc tgccattgtc gtcgatgctt tcttcaaaat cgccaagcca
      901 ttccaacata agatggacac gatgtaaggc tgcgtagatc gcgcttgcgg cgagattgta
      961 cgagaccagc gaatgctcgg atgaaagccc gccactcgcc aaacgtcccc tagcattcgg
     1021 caagtaggtc aatccaaaat tggcatgcaa ggcaaactgc ccgttaatct tcttgctaaa
     1081 aggcaaaccc cattcatagc cgacaactcc gtgcccggtg ccgcgcttgc gactgcctga
     1141 tggaaggatc aggctaaagc taggggcgaa agcaggcttc gtttcgtctt cctccaacaa
     1201 ctgatagcga tagctgagca aaacgtcgcc gagtccgttt tgccgatcac cattttcaac
     1261 caggtgatac gaagggatcg tgtaagaaaa ttgatggttc tgcgagaata tcggccattc
     1321 ctgagtaaaa ttaaactccc agccacgtcg ccgtgtatca ttgacgtaat gcgcatgaaa
     1381 gatatgttga accgtaccgg gctcttgatt gtacgcctcc tcgaggagaa aactgttgtc
     1441 ctcgacaacg tcaatcggtc gttccttctg ggccgccatg agagtggccg gagatatata
     1501 aactaccaac gcgattatga tccagtagaa gacgttaatc agcatggaag agatgttctc
     1561 atatctatca agcggagatt gaagccacct gttggatttt accagcgaag gctagcagct
     1621 cgagcttctg ttctcaggct agattatcac gaatgtcata caaccgaggc ggcaatgcta
     1681 ggcactatcc gggatagagt tctttgacga atccttcctt gatcaattgc tcaagcggcg
     1741 cgagatcgta gaattgttta ggatcggcat ctttggcctt gggatttcgc gtggcaacat
     1801 cttccaacac cgattccatc tctgcttggg tgatggtcgg cacatctcgg atatagccga
     1861 gcacgtaact cttaaaggcg ctgtttaaaa gttttgtatc tgtgttgcgc gtgtatttgc
     1921 ccagcactcg aacggtgaaa tcaggattgg ctttggcgta tttgattcct tcaagcgttg
     1981 ctttgagaaa gcgattcaga gtctgtggcc gatttttgat gaaccctccg gtggttacca
     2041 gagccgtgcc gggatagcga tactttatct cgccaaggtt caataattct ttgtagccaa
     2101 gctctttggc tctctcgaga gtcggcgatg agatcaagcc tcctttgata acgccttgct
     2161 gcatgaacag caaaatctcc ggcacgccgc cggtttgaat catcttgata tcggtcgtcg
     2221 gatcgaggcc aaactcgctg acagcctttc tcaggccaat atcggttagc gagccgaatc
     2281 ggcttactcc aaatgttttg cccttcagat cctccactcg gccaatatcg ggagttgtca
     2341 cgagactcat cggcacaacg ttgatcgtcg tgccgatgat cgtcacatcc gcgccggata
     2401 gtcgcgagag aacggcggca gcgccggagc cttgtacgat gtgcgtgtcg ccggagacca
     2461 gcgtggaaat cgcctgggcg ccggagacat aaatcgtatt tacatcaaga ccgtacttct
     2521 gatagatccc cgcttctttc ggtatccagg ttgaaaccga tgtcgcggct atcgatgaat
     2581 agcaaacgtt aagcttctcg ggttgggcgc catgcgcaac aggtagaatc cacgccgtga
     2641 tcaattgcgc agcgatgagc agagcgttag caatcttacc aggctttagg ggatttctca
     2701 ttcgcggtcc gtatgacgca accacctatc agcggcgact ataggaaagg cacccgacga
     2761 tttcaagcta cggcaccgtg gtgaggtctt tcatcgaaaa gcacaagcga aggacggcta
     2821 ggagcacgtc cgcgagctga aatgactacg ggcagggttc tctgacgcga gaacgtcaga
     2881 cgcaaagttt attgagggct agatttg
//
LOCUS       DASUTG010000895         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1323804, whole genome shotgun sequence.
ACCESSION   DASUTG010000895 DASUTG010000000
VERSION     DASUTG010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323804"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VF089_20795"
     CDS             complement(<1..320)
                     /locus_tag="VF089_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176453.1"
                     /translation="MKGYVTNIEDDTMKNKDFRRVLYTGKNSQLVLMSLRPKEEIGLE
                     THSLDQFIRVEAGRGIAVLDGVEHQIADGTAVVIPAGTKHNVINHSEREELKLYTLYS
                     PPEHR"
     gene            complement(385..1350)
                     /locus_tag="VF089_20800"
     CDS             complement(385..1350)
                     /locus_tag="VF089_20800"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX6176454.1"
                     /translation="MGVLVDGVWKDQWYDTENTGGRFVRWESRYRNWVTAEGAPGPTG
                     EGGFRAEPGRYHLYVSFACPWAHRTLIMRALKGLEGAISLSVTHWHMGENGWTFDPGP
                     GVIPDPIHNAKYLYQIYLADDPNASGRATTPVLWDKAQGRIVNNESAEIMRMFNDAFD
                     AVGAQPGDYYPAELRSDIDVLNERIYRAVNNGVYQAGFATSQEAYEEAAVNVFATLDQ
                     LEERLAASRYLFGDWPVETDWRLFTTLIRFDAVYVGHFKCNLRRLVDYPNLSGYTRDL
                     FQLPKIAETVNFEHIRKHYYTSHKAINPTGIVPIGPDLHFFTAHG"
     gene            complement(1390..1626)
                     /locus_tag="VF089_20805"
                     /pseudo
     CDS             complement(1390..1626)
                     /locus_tag="VF089_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
     gene            1957..>2567
                     /locus_tag="VF089_20810"
     CDS             1957..>2567
                     /locus_tag="VF089_20810"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6176455.1"
                     /translation="MIAGGQKKYFDPKVLAGISNLSLRARWVVEGIMSGIHRSRSKGF
                     SVEFEEHREYSPGDEIRRIDWKALGKFDRYFIKEYEDETNLRAYLLLDTSASMDYSSD
                     GISKFDYGCTLTASLAYLILRQQDAAGLVTFSNRIENFIPPRAKRDYLTQIVHALENQ
                     RPGGETNVGRILEEIAGQVKRRGIVILVSDLLDEPAQILKGLR"
BASE COUNT          571 a          733 c          683 g          580 t
ORIGIN      
        1 cggtgctccg gtggagaata aagcgtatag agcttcaatt cctctcgctc tgaatgattg
       61 atgacgttgt gtttcgtgcc cgctggaatc accacagccg tgccatccgc aatctgatgc
      121 tccactccat cgagaaccgc gataccccta cccgcttcga ctcgaatgaa ctggtcgagg
      181 ctatgagttt ccaagccgat ctcctctttc ggccgaaggc tcatcaaaac cagctgtgaa
      241 ttttttcccg tgtacaacac tcgtctaaaa tccttgtttt tcatcgtatc gtcttcaatg
      301 tttgtcacat aacctttcat atctgttccc tcctattatc gggtgtgact ctttacctga
      361 tggcggattc ttcctcatgt tttctcagcc gtgcgcagtg aagaaatgta gatccggacc
      421 gatggggacg ataccggttg gattgatcgc tttatgactg gtgtaatagt gcttgcgaat
      481 gtgctcgaag ttcaccgtct cggcaatctt gggtagctga aacagatccc gggtgtaacc
      541 cgatagattg gggtaatcga ccagccggcg cagattgcat ttgaagtggc cgacataaac
      601 ggcatcgaaa cggatcaatg tcgtgaacaa acgccagtcg gtttcgacgg gccaatcgcc
      661 aaacagatac cgggaagctg ccaggcgctc ttcaagctgg tcaagcgtgg caaacacgtt
      721 aaccgccgct tcctcgtaag cttcctggct ggttgcgaag cccgcttgat aaacgccatt
      781 gttaaccgcg cgataaatgc gctcgttgag cacgtcgatg tcgctccgca attcagccgg
      841 ataatagtcc ccgggctgcg cgccgacggc gtcaaaggcg tcgttgaaca tgcgcatgat
      901 ctcggcggat tcgttgttga cgatgcggcc ctgcgctttg tcccagagca ccggggtggt
      961 ggcgcgaccc gatgcgttcg gatcgtcggc gagatagatc tgataaagat acttcgcgtt
     1021 gtgaatcgga tcgggaatca cgcccggacc aggatcgaat gtccagccgt tctctcccat
     1081 atgccagtgc gtcaccgaca gcgagatcgc gccctctaac cccttcagcg cgcgcatgat
     1141 caaagtgcga tgcgcccagg gacaagcgaa agaaacatag aggtgatagc ggccgggctc
     1201 ggccctaaag ccgccttccc ctgtcgggcc gggagcgccc tccgccgtga cccagttgcg
     1261 gtagcgagat tcccaacgaa caaatcgccc gcccgtgttc tcggtgtcat accactggtc
     1321 tttccagact ccgtcgacca gtactcccat catttattcc ctattgagcg taggcgacga
     1381 gcgatgatgt tatttagtcc ggctttttgc tgcaaacgtc tgacgcgact cgctaggttc
     1441 gggctgaccg ctcgtgtaag tacttgccgg gcgtttctcg atcggcggcg gcttgccggg
     1501 caagggcggc agcgatttgg cttgctcaag atcgatgccg gctccctcgg cgacacgccg
     1561 gctataatct tcatcacaat gccaccagtg ccacaccatg cgcatagaaa tttctttggg
     1621 ccacctgccg gatctaagga tcgcgtgttc tactgtcacc taaacaacag cggggtggtg
     1681 aatcaatacc gtcctgggaa ataatggaac gtgacatccc ggcaatcacc ttgggaagct
     1741 tagcgttctt caacagacgc tgtacgcatc cgcatctgtc gccggcaggt tcacagcatc
     1801 catctcaaag ggtgctagcc ggtcacataa ctcgcgccag ctgcagagca aactcagccc
     1861 ggccgaaaaa tacaaaatcg gcagtttggg ccgccgaaac atagcttgaa aagctggcta
     1921 gattatcttt ttccgcggtg gtaaaggtgg agcaacgtga tcgccggtgg gcagaaaaaa
     1981 tactttgacc cgaaggtttt ggccgggata tccaatctct cactgcgggc gcgatgggtg
     2041 gtggaaggga ttatgtccgg cattcaccgc agccgctcaa aagggttcag cgtagagttc
     2101 gaagagcatc gcgaatattc tccgggcgac gagattcggc gcatcgactg gaaggcactg
     2161 ggaaaattcg accgctattt catcaaagaa tatgaagacg aaaccaacct gcgcgcctat
     2221 cttctgctcg acaccagcgc atcgatggac tactcgtccg atggcatcag taaattcgat
     2281 tacggttgca cgctcaccgc ttccctcgcg tatctcatcc tgcgccagca agatgccgcc
     2341 ggcctggtga ccttttcaaa ccgtatcgaa aacttcatcc cgccgcgcgc caagcgcgat
     2401 tacctgaccc agatcgttca cgcgctcgaa aaccagcggc ccggcggcga aactaacgtc
     2461 ggcagaattc tcgaagagat cgccggccag gttaaacggc gcggcatcgt aattcttgtc
     2521 tccgatctgc tggacgaacc ggcgcagatc ctcaaaggat tgcgctt
//
LOCUS       DASUTG010000896         5070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1620281, whole genome shotgun sequence.
ACCESSION   DASUTG010000896 DASUTG010000000
VERSION     DASUTG010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620281"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF089_20815"
     CDS             complement(<1..231)
                     /locus_tag="VF089_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176456.1"
                     /translation="MIKLLAVTGKKLTIPKLTTNGCERPRFDTVRRKRRILGSTSISF
                     AVYLEEIRDLGSQGSMIREIERWSTAPRIAKAI"
     gene            complement(455..1306)
                     /locus_tag="VF089_20820"
     CDS             complement(455..1306)
                     /locus_tag="VF089_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176457.1"
                     /translation="MPFANVRGVTINHKILGTQGPWVALSPGGRRDMSAIEPLANHVA
                     QLGFRVLILDRRNCGASDVVIAGEDSEYEIWADDFHELLSQLGALPAFIGGSSSGCRT
                     SLLFALRHAEAVRGLLLWRVTGGRFACDRLAQEYYGQYISAAQEGGMAAICEMEHWKE
                     RIEARPENRKRLMNMEPSQFISVMAHWREYFLRGADLPVIGASEAELRSIKAPAIIIP
                     GNDNTHSQKTGERLGQILPNAQVHILFPRHYDMDLSPREEWDEKAPEMASRFAEFMAR
                     SKQATPE"
     gene            complement(1412..2434)
                     /locus_tag="VF089_20825"
     CDS             complement(1412..2434)
                     /locus_tag="VF089_20825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176458.1"
                     /translation="MILKTFLLLAAISFLSAGKIELFAAQSAFEGKAIRIVVGFSAGG
                     GFDTYSRAIARHMGKHIPGNPSIVVENMTGAGSIVAANHLYRVAKPDGLTIGNFHGFQ
                     ILNQVLGAPGIEFDARKIEWLGVPVQDTGACALTKASGIGSIEQWQAAKQPVKLGGVG
                     PGDASYGMARVLKEVLRLPVQPIPGYKGTADIRLASESGEIAGGCWQWESIKVIWRKG
                     LETGDANVVLQLVPKPHPELPKVPLAVSLAKTDEARTLLQAAVHDPNSITRPYSLPPG
                     TPQDRVKILQKAFTDTMKDPDFLADAKKSQLDIDPLSPEEVEKTVARFFKLDAALIAK
                     LREILK"
     gene            complement(2538..2906)
                     /locus_tag="VF089_20830"
     CDS             complement(2538..2906)
                     /locus_tag="VF089_20830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176459.1"
                     /translation="MSRIRHIAITTKDVGRLVRFYTTVFGLKVVPGRGTGTYLSDGHV
                     NLAILPLEPERETEGPYLKEGFYHFGFQVDDVDALRPISEQMGATSKIEKRPENREVE
                     ARVYDPDGNPVDLSKRGWPI"
     gene            3339..4364
                     /locus_tag="VF089_20835"
     CDS             3339..4364
                     /locus_tag="VF089_20835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176460.1"
                     /translation="MKEYHRGMWLALLMCLAFCSTLSAAEDFYSGKTIRLIVATTPGG
                     GFDAYSRMLSRHMGKHIPGKPTFVVENMPGAAFMIGTNYLYKQAKPDGLTLGNWIGTL
                     VLHQVIGKKGIEFDARKFEYVGAPVKNHDTCVMSRASGIDSAKKWLSSPTPVKLGATP
                     PGSTPYDNAAVLKEALGLPLQLVSGYKGTAEIRLALDGGEVSGLCGLAWASTKVTWRK
                     QLESGEIKVVLQNTAVAHPELPDVPTAISLAKTDASRKLIELALHDVGAITYLYTFPP
                     GTPKDRVQLFRQGFQATLKDPEFIAEAKKGNFELDPVPGEELEKIVNGFFKADTATIN
                     RLRTLLK"
     gene            4585..5007
                     /locus_tag="VF089_20840"
     CDS             4585..5007
                     /locus_tag="VF089_20840"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX6176461.1"
                     /translation="MEHQKLQILFLGLFSLLSLSGCAAVGMTLFGVGAGVATGTSVSY
                     TLDGIAYRTFTAPISQVEGATRTALDRMGIKIQATSKTEQGKAIKAMSNDREIEIELE
                     MVSAKTTRIRTVAKQGIFFKDRATATEIIMQTERVLGV"
BASE COUNT         1210 a         1332 c         1343 g         1185 t
ORIGIN      
        1 tattgcttta gcaatccttg gggcggtgga ccatctttcg atctcccgaa tcatgcttcc
       61 ctgactgccc agatcccgaa tctcttcgag gtacaccgcg aagctaatag atgtacttcc
      121 taggatgcgc ctttttctgc ggacggtatc gaatcgcgga cgttcacaac cgtttgtcgt
      181 aagtttcgga atcgttaact tttttccggt caccgcaagc aacttgatca ttcagccaaa
      241 caactgccgc gcaagcggaa gttattgccg cggacatggg gagccttggg tcgattcttg
      301 tagctaccca aaccaggcga gagacgcttt cacttaataa aacgagatca taaacggaac
      361 attcggctgt ttgagcagag tggacccaag tgaaggtaaa tggttttccg agactcagaa
      421 gccggcatta gcttcgccgc aagttcagaa aatctcactc gggggttgcc tgtttgctac
      481 gggccatgaa ttctgcgaag cggctcgcca tttcgggagc cttctcgtcc cattcctccc
      541 gcgggcttaa gtccatgtca tagtgtctgg gaaaaagaat gtgtacttgg gcgttaggaa
      601 ggatttggcc caggcgctcg ccggtcttct gcgagtgtgt gttgtcattg ccaggaatga
      661 tgatggccgg cgctttaatt gatctgagct ccgcctcgct tgctccgata accggcaggt
      721 cggcgccgcg caggaagtac tcgcgccaat gagccatgac gctgatgaac tgagaaggtt
      781 ccatgttcat tagccgcttg cgattttcgg gtcttgcttc gatgcgctct ttccagtgtt
      841 ccatctcaca aatggcggcc attccgccct cctgcgccgc gctgatgtac tggccgtaat
      901 actcttgagc cagacggtca catgcgaagc ggccgccggt gacgcgccac aacaggagac
      961 cccgaactgc ttcggcatga cgcaaggcaa agagaagcga ggtgcggcaa ccggaagaac
     1021 tgcccccgat gaaggcggga agcgcgccca gttgtgagag caattcgtgg aaatcgtcgg
     1081 cccagatctc atattccgag tcctctccgg cgatcaccac atcggaggcc ccgcagttgc
     1141 gccggtcgag gatgagaaca cggaatccta gctgagctac atgattggcc agcggttcaa
     1201 tcgcgctcat gtcgcggcgc ccgcctggcg acagcgctac ccatggtcct tgagtaccga
     1261 ggattttatg attgatagtg acgccgcgta cattagcaaa tggcatattt tcctccatcg
     1321 ctgacgaatc ggtaggagag ggcgccgggg taaggatcgt gattacgtca acagctcaac
     1381 tgaacccacc cgcgattgca tcaaatagtc gtcatttgag aatctcgcgc aacttagcga
     1441 tcagagccgc atctagcttg aagaaccttg cgacagtttt ttctacttct tccggcgata
     1501 acgggtcgat gtccaattgg gatttctttg catcggccaa aaaatccgga tctttcatcg
     1561 tgtccgtaaa tgccttttgc aatattttta cgcggtcctg cggcgtaccc ggtggcaatg
     1621 agtacgggcg ggtgatggaa ttcgggtcgt gaaccgccgc ctgcaataag gttcgagcct
     1681 catcagtctt agcgaggctg accgcgagcg gcactttggg aagctcggga tgcggttttg
     1741 gtacgagctg gagcactaca ttcgcatcgc ctgtttcgag gcccttgcgc cagatcacct
     1801 tgatcgattc ccattgccag cagccgccgg caatctcgcc gctctcagac gcaaggcgaa
     1861 tatcggctgt tcccttgtaa ccggggattg gttgaaccgg taggcgtagc acctctttca
     1921 gaactctcgc catgccgtaa gacgcatctc ctggtcctac accgccgagc ttgaccggct
     1981 gttttgccgc ctgccattgc tcgatgctgc caatgccgct ggccttggtc aaagcgcagg
     2041 caccggtatc ttgcacaggt actccaagcc actcgatctt gcgtgcatca aactcaattc
     2101 cgggcgcgcc caagacctgg tttaaaatct gaaaaccatg aaagttgcca atcgtaagcc
     2161 catcaggttt cgccactctg taaagatgat tcgctgcaac gatacttcct gctcctgtca
     2221 tgttctccac gacgatggat ggattgccgg ggatatgttt ccccatatgg cgggcaatgg
     2281 ctcgagaata ggtatcgaag ccgcccccag ccgaaaaccc gactacgatt cgtatcgctt
     2341 taccttcgaa tgcggattgc gcggcgaaaa gctcgatctt accagcgctc aggaatgaaa
     2401 tggcagcaag taataagaag gttttcagaa tcatcaagaa gcctcctgaa tagtctcgca
     2461 tcgatcaagt ggagccagtc gtgttggcgc cgcaaaaaca cttattgcaa ataggccgtc
     2521 tgaggctacg tgaagcgcta aatcggccat ccgcgtttcg aaagatctac aggattgccg
     2581 tccggatcat agacacgcgc ctcgacctcc cggttctccg gccttttctc aatcttcgaa
     2641 gtagcgccca tttgctcgct gatcggtcga agtgcgtcca cgtcatcgac ctggaagcca
     2701 aaatggtaaa acccctcctt aagatacgga ccttcggtct cccgctcagg ctcgagtggc
     2761 agaatcgcca ggttgacgtg accgtctgaa aggtaggttc ctgtcccgcg acccggcaca
     2821 actttcagac caaagactgt agtataaaat ctgacgagac gaccgacatc cttcgtagta
     2881 atcgcgatgt ggcgaatgcg cgacatagac ttagcctcca actgaagtgt gtgaaaatca
     2941 tttctctatt tgtgcttatc ggctgtgatg caagcaaatc taccttcggc taatcttcgg
     3001 gaaataccat aggtccgatc gatgacaagc gtcaagctgt ttagccagta ttcggcaact
     3061 tatgccatat taaaaagcaa tagaacacag ccgcgagaag ataacacgac aggtctactt
     3121 ggctatcgag cagattgatg caccggccat caactgctta gacgaatcat gctgagtttc
     3181 acgacagcca aggctgccga acaatcgatc tctgcgcttt cagcgcgtgt gcagtgatcc
     3241 ttggtttagt tcttaaggat ttccttggta ctcagaagat catttgataa aagattccat
     3301 tgatcaacgg agttaatgga attactcgga ggggctctat gaaagaatat catcgcggca
     3361 tgtggttggc tctgttaatg tgcctggcat tttgttcgac gctgtctgcg gcggaagatt
     3421 tttacagcgg caaaacgatt cgcctgatcg tagcgaccac gcccggcggc ggattcgatg
     3481 cctattctcg tatgctctcc cgtcacatgg gcaagcacat tccaggtaag ccaacctttg
     3541 tcgtggagaa tatgccgggc gcggctttca tgattggcac caattatctg tacaagcagg
     3601 ctaaacccga tgggctaact ttgggtaatt ggatcggcac gctggtttta catcaagtga
     3661 taggaaagaa agggattgag ttcgacgcgc gcaagttcga gtatgtcggc gcgccagtga
     3721 agaatcatga cacctgtgtt atgagtcgag ccagcggcat cgatagcgca aaaaagtggt
     3781 taagctcacc gacaccggtg aagctgggcg caacgccgcc gggttcgacg ccgtatgata
     3841 acgcagcggt actgaaagaa gcgctcggac tgcccctgca gcttgtctca ggctacaaag
     3901 gcaccgcgga gatccgcctg gccctcgacg gaggtgaggt ttcgggtctt tgcggtctgg
     3961 cttgggcatc gacaaaagta acctggcgca agcaattaga gtcgggggag ataaaagttg
     4021 ttttgcaaaa cacagctgtg gctcatcctg aattgcccga cgtgccaacc gctatcagtt
     4081 tggccaagac cgacgccagc cggaagctga tcgaattagc gcttcatgat gtcggagcca
     4141 ttacctatct ctacacattc ccgccaggca caccgaaaga tcgcgtgcaa ctcttccgac
     4201 aaggatttca ggcgacattg aaagatcctg aattcattgc cgaggccaag aaagggaact
     4261 tcgagctcga tccggtaccg ggcgaggaat tggaaaagat tgttaatggc ttcttcaaag
     4321 ccgatacggc gacgatcaat cgactgcgca ctctgcttaa ataagtacct aaagccgggt
     4381 agaatcgcta tggcaataaa cgatgcacgc ttcgaaggtc aacgcccgtc gccgagtagg
     4441 atcatgtaga aaatcaccga gtccatcaca attttgctta cctggacttt tctgattttg
     4501 tccttccctg cttaagaact cccataggag gcaattgccg ccagttgttt cccgtggcgt
     4561 atatattgcc tttgatgctc ccgcatggag catcaaaaac tgcaaatact tttcctaggg
     4621 ctattttctt tgctttcatt aagcggctgc gcggccgtgg gcatgacgct attcggtgtc
     4681 ggtgccggcg ttgctactgg tacttcagtt tcttacacct tggatgggat cgcttaccgg
     4741 acatttaccg ctccgatatc gcaggtcgaa ggagctacac gtacggccct ggatcgtatg
     4801 ggaataaaga tccaggcgac ttccaaaaca gagcagggga aggcgatcaa agcaatgagc
     4861 aatgaccgtg agatcgagat cgaactagaa atggtaagtg ccaaaacaac caggattcgt
     4921 acagtggcaa aacagggcat cttcttcaag gatcgcgcaa ccgcgactga gatcatcatg
     4981 caaacagaac gggtgctagg cgtgtgaggc tgaacaggat aaccaaaggc aggagcaaat
     5041 agagaggcct tatctcgacc ggaggaagtg
//
LOCUS       DASUTG010000897         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1175196, whole genome shotgun sequence.
ACCESSION   DASUTG010000897 DASUTG010000000
VERSION     DASUTG010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175196"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..1617)
                     /locus_tag="VF089_20845"
     CDS             complement(622..1617)
                     /locus_tag="VF089_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176462.1"
                     /translation="MAVLQIDLACGDYDIVRPLRDGIIRAEGMEINFVTINKPPEVHW
                     RMGINEEFDAAEMSLGSFVAGKAHGKYPFIAIPAFVYRKFRHSGAYVNVKAGIEKPED
                     LKGKRVGVPEWQMTATVWLRGFLQDDYGVGPQDVSWLTGGLESSGRKEKVPLQLPPQI
                     RVEAIAEGKNLSAMLVDGELDALMTAQVPGPYLKRLPQVRRLFARSREIERDHFKRTG
                     IFPIMHVMVLREDIYKRHPWVAQSLYKALLEAKTHCIETLYNNDSLHAALPWAAHHVE
                     ETRQLMGHDYWPYGVEANRRTLEVFLRYSREQGLSNRNMDVDELFAKETLHVFHV"
     gene            complement(1697..>2730)
                     /locus_tag="VF089_20850"
     CDS             complement(1697..>2730)
                     /locus_tag="VF089_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176463.1"
                     /translation="GSANRRETSPPQEEQATWNTMAQKFTSTLILFGIMVFSLSSPIC
                     ADAQELKKVKIGYPAFSLTFLTFFVAKDAGIFKKHGLDVELIQLAGAVQTSALVAGEI
                     DHLTGITGPLVAAARGLPFKGILVTHERPLFWLIANPEIRRAEDLIGKTVAVDRLATL
                     QDIIARDLIKRKGVSPERVTFVQTGSVSNSVQSLSQNSVAAALLSLPHNVVMMQRGYR
                     EIASAAEFNMRSASGGIATREAKLKQDPAGIKALIRALFDSMDFNRREKTWMVNYIQN
                     RWKLAPKAADEAYRFWLEGLTPDGKIPLKDLQDIYDLAYSTQMIPTPVPVTKVMDYSL
                     TDEVLKERK"
BASE COUNT          631 a          715 c          711 g          673 t
ORIGIN      
        1 ggcgcgccgt tgaccttact gtgagaaaaa accagctgcc aaaaactcgg tcgctctaaa
       61 gttttttcga gggttttcac ttgttctctc cttaaatgat actttggttt tgctctgcta
      121 attgatcgcg tccctccgat acagcagacg gtgtgccggg cctgcgaaat ccactgtaag
      181 ccaaaattgt cggaattttt accgagctac tcccaggata gctccggcaa gggcgacccc
      241 tttgagggtt acggaggctt atcggtcaga actgccagct gttatctcgg tgattttggt
      301 caagagggcc gcagcatttc acacgatctg acccatcgca gcatatgccc gcagttatca
      361 ggtcacattc tgcctatcca catcagcagt gaattctaca aatcgatgaa cgtgacacga
      421 ttggttgcaa gataacctcg cgtcgggcga atgcttgatc tgagttgggc aagacggtcg
      481 agaagttaat catgaaaggg agaacccctc tgctagagca tttgcacccg ggaggtatgc
      541 gcggtactta gcagcaggcg gagcaataca cgattcactt gcgcgtatct aaagcaccgc
      601 acacagtaat gttcttccgt tctacacgtg aaagacatgc agcgtctctt tggcgaaaag
      661 ttcatcgaca tccatgttcc ggttgctcag gccttgttct cgggagtaac ggaggaatac
      721 ttccaacgtc ctccgattag cttcgacacc gtagggccaa tagtcgtgcc ccatcaattg
      781 tctggtttct tcgacgtgat gcgcagccca aggtagcgcg gcgtgaagtg agtcattgtt
      841 gtaaagagtt tcgatgcagt gcgtcttggc ctcgagcagt gccttgtaga ggctctgcgc
      901 aacccaggga tgacgtttat agatatcttc acgaaggacc atcacgtgca taatcgggaa
      961 aatccctgtt cgtttaaaat gatctcgttc tatctcacgc gagcgggcga agagtcgccg
     1021 tacttgcggg agtcgcttca ggtaggggcc aggcacctgt gccgtcatca gggcatcgag
     1081 ttcaccatcc actagcatgg cggaaagatt cttgccttcg gcgatcgctt caactctgat
     1141 ctgcggcggt aactgcaatg gcaccttctc tttgcgcccg gaggattcaa gccctccggt
     1201 cagccatgat acgtcttggg gccccacgcc ataatcgtct tgcagaaacc cccgcaacca
     1261 cacggtggca gtcatctgcc actcaggcac gccgacgcgc ttccctttta aatcttctgg
     1321 cttctctatc ccggctttga cgttgacgta agcgccagag tgccggaact tacgataaac
     1381 gaaagcggga atggcaataa acggatactt gccgtgcgct ttacccgcga caaaactgcc
     1441 aagtgacatc tcggcggcat cgaattcctc gttgatcccc atgcgccaat gaacctctgg
     1501 cggtttattg atcgtgacga agttgatttc cattccttcc gctctgatga tgccgtcgcg
     1561 cagcgggcga acaatgtcat agtcgccgca cgctaagtca atctgtaaaa cggccatgat
     1621 tccactcctg aagcaggttg ggtcaaagaa cggtaaagtt aaaagcagag tggatgaaaa
     1681 tgtcccgtgg aaagaattac tttctttcct tcaacacttc atcggtcagg gaatagtcca
     1741 tgactttggt aacgggcact ggcgtcggga tcatctgtgt ggagtaagca aggtcataga
     1801 tatcctgtaa gtcctttaaa ggaatcttgc cgtccggcgt cagtccctcc agccaaaatc
     1861 gatacgcttc atccgcggct ttaggcgcca gtttccacct attctgaatg taattcacca
     1921 tccacgtttt ctccctgcga ttaaaatcca tcgaatcgaa gagagcgcgg atcaaggctt
     1981 tgatgccggc gggatcttgt ttcagctttg cttcgcgggt tgcgatgccg ccggaagcgg
     2041 agcgcatgtt gaactccgca gcagaagcta tctctcgata tcctcgctgc atcatgacga
     2101 cgttgtgcgg cagcgagagc agggcggcgg ctacggaatt ttgactcagc gattgaacgc
     2161 tattggaaac cgacccggtt tgaacaaagg tgactcgttc aggactcacg ccttttcttt
     2221 tgatcagatc tctcgcaata atgtcttgca gggtagcaag acgatcgacg gcgacagttt
     2281 tgccgatcaa atcttccgcg cggcgaatct ccgggttggc aatgagccaa aaaagcggtc
     2341 gttcatgcgt caccaggatt cctttgaagg gcaggccgcg cgcggcggca accaacggcc
     2401 cggtgatacc tgtgagatgg tcgatttccc ctgccaccaa ggccgatgtt tgaaccgctc
     2461 ccgccagctg aatgagttcg acgtcgagtc catgcttttt gaagatgccg gcgtccttgg
     2521 caacgaagaa agtgagaaac gtaagactaa aagcagggta gccgatttta actttcttaa
     2581 gttcttgcgc atcggcgcaa atcggggatg acaggctaaa gaccattatt ccgaacagaa
     2641 ttagtgttga agtgaatttc tgtgccattg tattccaagt cgcctgctct tcttgcggcg
     2701 gtgaagtttc gcgccgattt gctgaaccgg
//
LOCUS       DASUTG010000898         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_435889, whole genome shotgun sequence.
ACCESSION   DASUTG010000898 DASUTG010000000
VERSION     DASUTG010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435889"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1099)
                     /locus_tag="VF089_20855"
     CDS             complement(41..1099)
                     /locus_tag="VF089_20855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176464.1"
                     /translation="MKRRSLKFVAGIVLTAAWAALPVFDSAALAQADFYKGKTIRIMV
                     GSTPGSFYDLWGRLLARYWGKQIPGSPEVIVQNMPGAGSLTATNYVYAVAKPDGLSMV
                     LPNNSIYIEQLVERKEVQFDLRKFHWIGSASQDSIIFYMRADTPFKTIGDIVKAKQPP
                     SCGGSGTTSSDYLVAKILELTVGANINSVLGYPGGNEADLAVEKGEIACRAHTLAAHF
                     GREPFNSWHKKGFDRHLLQTGRKRDPRAREAPTIYEILEEFKVPSGNQRIVQAMLSGG
                     EFGRPFLVTPGTPPDRVKILRESFVKALKDPELLDDAKKAKMEVDYTSGEELEALIKD
                     VLDQPPEIVQQVRKFMAK"
     gene            complement(1306..2037)
                     /locus_tag="VF089_20860"
     CDS             complement(1306..2037)
                     /locus_tag="VF089_20860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6176465.1"
                     /translation="MADYILKQKDADVTTLTLNRPDIGNRQDDATLAQLAQMIDAAAK
                     ESRLILFKATGEDFCLGREAMGKPGPALEAYQMRERADTIFDLYEAFRRAKVPIIGVV
                     QGRAAGLGCALAALCDITIASEKARFQLPEMAHNIMPTIAMSALVDRVPRKAATYLIY
                     STQEIDANKALMFGIISDVVPAAELDSAVGKLVERLRQTPLAALMAVKEYGRSAFNMN
                     TQAATDFARNLHATVNTFSGMKPRA"
     gene            complement(2124..>2614)
                     /locus_tag="VF089_20865"
     CDS             complement(2124..>2614)
                     /locus_tag="VF089_20865"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6176466.1"
                     /translation="VETLCNSPAMDPESLPPAYRKESMCNVNAAVASSLIKSLQDNLA
                     ARRVRVRAYQELLGNERLILVPHREGSACLTQVIGVLPRSMGHDPSSDLIAILRRAGY
                     EVQGSYIPIHLLAPYQHLAVRSFPLAEKIWTNLVELPCEPDVSLEHVGRIASIVKQTI
                     TC"
BASE COUNT          582 a          740 c          685 g          607 t
ORIGIN      
        1 tccgatcttt tcgtccgttg acctgctgct tcgccggtgt ttacttggcc ataaatttcc
       61 ttacctgctg cacgatctcg ggcggttgat ccaacacatc tttgatcaaa gcttccaatt
      121 cttcgccgga agtataatcg acttccatct tggctttttt ggcatcgtct aacagctctg
      181 gatctttcaa ggccttgacg aaagattcac gcagaatctt cacccggtcc ggcggcgtgc
      241 cgggtgtcac caagaacggg cggccgaact ccccgccgga caacatggct tggacgatgc
      301 gctgattacc gctaggaact ttgaattcct cgaggatttc gtaaatcgtc ggcgcttcgc
      361 gcgcgcgcgg atcgcgttta cgtccggttt gcagcagatg acgatcgaag cccttcttgt
      421 gccagctatt aaagggctcg cggccgaagt gagccgccag ggtatgagcg cggcaagcga
      481 tctcaccttt ctcaactgcc aagtctgcct cattgccgcc gggataaccc aaaacagaat
      541 tgatgttcgc gcccaccgtc agttccaaaa tttttgcgac cagataatcg gagctggtgg
      601 tcccggatcc gccgcagctg ggcggctgtt tcgctttgac aatatcgccg atggtcttga
      661 acggcgtatc ggcacgcata tagaaaatga tcgaatcctg cgacgccgaa ccgatccagt
      721 gaaattttcg cagatcgaac tggacttcct ttcgctcgac cagttgctcg atatagatgc
      781 tgttgttcgg caataccatg ctcaagccgt ccggtttagc caccgcatac acatagttcg
      841 tggcggttag cgaccccgcg cccggcatgt tttgcacgat cacttcggga ctgcccggaa
      901 tttgtttgcc ccagtagcgc gcgagcaggc gtccccacag gtcataaaaa cttcccggcg
      961 tcgatccgac cataatgcga atggttttgc ccttataaaa atcagcttgt gccagtgcgg
     1021 cagaatcaaa tactggcagc gccgcccacg cggcggttag aacaatccct gcaacaaatt
     1081 ttaaactgcg acgcttcaca agacctcctg ggttagagag ctgcgcgaca tcattcaata
     1141 gcggaacaac gtgtcaaata cagacgcttg ttcatactag gtcagaacat gatgaaatgt
     1201 gctcatgcgc cgccggagtt cacggtggtg tgcggctgac cggctgttct tacgctccgc
     1261 gttacgacac cacgttccga acctctaccg atatcagccg tctagctagg ctcgcggctt
     1321 cattcccgaa aacgtattaa ccgtagcatg aagattgcgg gcaaagtcag tggcggcctg
     1381 ggtgttcata ttgaaagcgg accggccata ctcttttacc gccatcaagg cagcgagcgg
     1441 cgtctgtcgc agccgttcca ctaacttgcc cactgcggag tcgagttccg cggcgggcac
     1501 gacatcgctg ataatgccaa acataagagc cttattggca tcgatttcct gggttgaata
     1561 gatcaggtaa gttgccgctt tgcgcggtac acggtcgacc agcgccgaca ttgcgatggt
     1621 cggcatgata ttgtgggcca tctccggtaa ctgaaaccgc gccttctcgc tcgcgatcgt
     1681 gatatcgcac aacgccgcca gcgcacagcc caaacctgcg gcgcggcctt gaacaacgcc
     1741 gatgatcggc actttggcac gacggaaagc ctcgtaaaga tcgaagatgg tatcggctct
     1801 ctcacgcatt tgataagctt cgagcgccgg accgggttta cccatcgctt cgcggcccag
     1861 gcaaaagtcc tcgcccgtgg ctttaaataa gatcagccgc gactccttcg ccgcagcgtc
     1921 gatcatctgc gcaagctgag ccaaggtggc atcgtcctgc cggttgccga tgtcaggccg
     1981 gttcaatgtg agggtggtca cgtctgcgtc tttttgctta aggatgtaat ctgccacgat
     2041 cattgcctcc gtaacttcga tgaaccgact ttagaagcct caactgcatt ttgtcaacga
     2101 aatcttccgg tcctatcgga tcatcaacac gtgatcgttt gcttcacaat ggaagcgatt
     2161 cgcccaacgt gttcgaggct gacgtcgggt tcgcaaggca gctcgactag attcgtccag
     2221 attttctcgg cgagaggaaa tgaacgcacc gccagatgct gataaggtgc cagcaagtgg
     2281 atcgggatgt aacttccttg aacttcgtaa ccggcgcggc gaaggatcgc gataagatcg
     2341 gaggaaggat catgccccat ggatctaggc aacactccaa tgacttgagt caggcaggca
     2401 gagccttccc ggtgcggcac caaaatcaaa cgttcatttc caagcaattc ttgataggcc
     2461 cgaacgcgca cccggcgcgc ggctaaattg tcttgcaaac tttttatgag actcgaagcg
     2521 accgcggcgt tgacattgca catggattct tttcgatacg ccggcggcag tgactcagga
     2581 tccatggctg gcgaattaca tagagtttca acga
//
LOCUS       DASUTG010000899         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1030806, whole genome shotgun sequence.
ACCESSION   DASUTG010000899 DASUTG010000000
VERSION     DASUTG010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030806"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2449
                     /locus_tag="VF089_20870"
     CDS             185..2449
                     /locus_tag="VF089_20870"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6176467.1"
                     /translation="MPFSNKASRVDGFEKVTGEAKYTGDLNLPGLLEAKVLRSPYPHA
                     RIESISTEKAAARCGVVAILTRDDVKDINPFYGNCLRDRALVALDKVRFVGEPVAVVA
                     AEDPMIAEEALALIDVRYQELPCLATVEDALAADAPLIHESTAGTGEFHDIATVGRAM
                     RPNVCHDEFFERGDVTRGFAESDQVIEEAFEFPMIYQYAMEPHTSIARVNDCGITLWS
                     SSAHPFLVRSELAHMFGLPHAKVEVVVPYVGGAYGSKSYFKIEPLIVAMARKTAGRPV
                     RLAQSVPEAMLTTRRHSATVRIKTGFKRDGTLVARDAQVVMDTGAYADNGPRVAKRAI
                     SRMIGPYKLGACKVEVAAVYTNTVPAGSMRSIGGPQTIWALESHMDSIAHRLGLDPLE
                     FRMRNLLKRGEELKGGATPIDADLRQGTKVAADYVGWGERFKRIGKGRGVAVGVSDSE
                     AMPVSVALVRLLADGSILLLAGTTEVGQGARTILSQIVAQELDVPVESVIMRGTDTLA
                     TPFDRSTGASRSTTVMGSAVKAAALDLRQQLIAAAAEAFGANASSIVLQNGAADVGDR
                     SLTYGEVVGRYFGMPGGELIGRGTMRPGQGFGPKLPLFWEPGMGAAEISLDSETGAIQ
                     VDRYITVADVGKAINFVQAEGQDEGAAIQGLGHTFFESLQYENGQPVNANLVDYRVPR
                     FTDMPAHFDSALIENEDGPGPYGAKGMGESGIVSIAPAIGNAIFQATGVRIRTLPLTP
                     ERVWRALNDAKRGM"
     gene            2471..3745
                     /locus_tag="VF089_20875"
     CDS             2471..3745
                     /locus_tag="VF089_20875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX6176468.1"
                     /translation="MERNQVFDRVSAIIERARAELIDLCLQLGNISSPHGQERDLGEA
                     VLDWLKQYQIPGQLQFITQRSVNAVATMTGSGQGPSLIFNAHMDTGPELPADASEDAR
                     KLETAWVEGEMLFGKGMINDKAQLCAFMIAMSALRRAGVQLNGDLTLTAVASETGNPS
                     IDEFQGIDFPGEGFGTKWLVDRGAIADYALVGETSGFGIVQAECGAAWFKIRVKGREV
                     YTPRYERGSSMQENPNAFVKAAHVIQAFEAWAIQYEKRETIEFAGGTIIPKAQIVEVR
                     GGAGVGRPNPTCDIFTDVRIAPGKNPLTVQRELAAAIAKVKLDCEISLFQYSRGHIAK
                     NAGPLIDAVRSAHRYLFGAEPPQPPSAETSMWRDLNVFNEIGIPSICYGPPRQRERLS
                     GAQNRAMKIDDLVEATKVYALSAMILCGVQSS"
     gene            3831..4430
                     /locus_tag="VF089_20880"
     CDS             3831..4430
                     /locus_tag="VF089_20880"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6176469.1"
                     /translation="MKFCLQLNPQVSVDKAAAQLMPTLIEQVRVADGVGFDAFSMGDH
                     YNIPGLQRLNQIPALARLCGEASRCAVGTAVMLLGLRHPVTVASELASLDVINEGKSF
                     FAFGLGYRDDELNAFNLTKAQRFHRFVEGVEIIKRLWTENNVHYDGTAFKLKNVSVDP
                     KPLQKPRPPIWIAANSDAAVARAAAIGDGWMMGAAFRHR"
BASE COUNT          948 a         1264 c         1372 g          942 t
ORIGIN      
        1 gtggcttgtg tggtcgcttg tttttacttc gctgactgcg attcaggctg cggagcgccc
       61 tttgggttga gaaacaaggc tgaataaggc taaataggcg aaatgacgtt gacgccccca
      121 gaaaacttga gctccatgcg aattgttgcc gaacggtcgg ttatctgatc cgcagatcag
      181 ccctatgcca ttctctaaca aggcatctcg ggtcgatggt ttcgagaaag ttaccggcga
      241 ggcgaaatac actggcgatc tgaatctgcc cgggttgctg gaggcaaaag tcctccgcag
      301 cccgtatcct cacgcgcgca ttgaatcgat tagcaccgag aaagctgctg ctcgttgtgg
      361 agtggtggcg atactgactc gcgacgacgt caaagacatc aatccttttt atggcaattg
      421 cctgcgcgat cgcgcgttgg tcgcgctcga caaggttcgt tttgtcggcg aaccggtggc
      481 ggtggtcgct gctgaagatc cgatgatcgc cgaggaagcg ctggctctga tcgatgtgcg
      541 ctatcaagaa ctgccgtgcc tcgcaacggt ggaagacgcc ctcgccgcgg acgcgccgct
      601 tatccacgaa agcacggccg gcaccggcga attccacgac atcgccactg tcggcagggc
      661 gatgaggccc aacgtctgcc atgacgaatt cttcgagcgc ggcgatgtta cccgcggctt
      721 tgcggaatcc gaccaagtta tcgaggaagc cttcgagttc ccgatgatct atcagtacgc
      781 catggagccg cacacttcga tagcgcgcgt taacgactgc ggcattaccc tgtggagctc
      841 ttcggctcat ccgtttctgg ttcgctccga actggcgcac atgttcggct tgccgcatgc
      901 aaaagtcgaa gtcgttgtcc catacgtcgg cggcgcttac ggcagcaaat cctatttcaa
      961 gatcgagcca ttgatcgtcg ccatggcgcg caagaccgcc ggtcggccgg tgcgcctggc
     1021 gcaaagcgtg ccggaagcga tgttgacgac gcggcgccac tccgccactg tgcgtatcaa
     1081 gacggggttt aaacgcgacg ggaccctggt tgcgcgcgac gcccaagtgg tgatggatac
     1141 cggcgcctac gccgataacg gcccgcgcgt ggccaagcgg gcgatcagcc gtatgatcgg
     1201 tccttacaag ctgggcgctt gcaaagtcga ggtcgcagcg gtctacacca acacggtgcc
     1261 ggccggttcg atgcgttcga tcggcggccc gcagacgatc tgggccttgg aatcgcacat
     1321 ggacagcatc gctcaccggc tcggcttgga cccgctggaa tttcgcatgc gcaatctact
     1381 caagcgaggt gaagagctaa aaggcggcgc cacgccgatc gacgccgatc tgcggcaggg
     1441 cacaaaagtt gccgcggatt atgtcggttg gggagaacga ttcaagcgga tcggcaaagg
     1501 gcgcggtgtt gccgttgggg tttccgattc agaagcgatg ccggtttcgg tggccctggt
     1561 gcggcttcta gccgacggca gcattctctt gctcgccggc accactgaag tgggtcaagg
     1621 cgcgcgcacg atcctcagcc agatcgtcgc gcaggagctg gatgttccgg ttgaatctgt
     1681 gatcatgcgc ggcaccgata cgctggcaac tccatttgat cgttctaccg gcgctagccg
     1741 ctcaaccaca gtcatgggaa gcgcggtcaa agctgccgcg ctcgatttgc ggcaacagtt
     1801 gattgccgcc gccgccgaag cgttcggcgc caacgctagc tcgattgtgt tgcagaacgg
     1861 cgcggccgac gtcggcgatc gtagtctgac gtacggcgaa gtagtcggca ggtactttgg
     1921 catgcccggc ggcgaactca tcggccgtgg caccatgcgt ccgggtcagg gcttcggtcc
     1981 gaaattaccg ctgttctggg aacccggaat gggcgccgcg gagatttccc tcgattctga
     2041 aaccggagcg atccaggtcg atcgctacat cacggttgcc gacgttggca aggctatcaa
     2101 ttttgtgcaa gccgaaggcc aggatgaagg cgccgcgatc cagggattgg ggcacacctt
     2161 ctttgagtca ctgcagtatg agaacggcca gccggtaaac gccaatctgg ttgactatcg
     2221 cgtgccgcgg tttaccgata tgccggcgca cttcgattcc gcattgatcg aaaacgaaga
     2281 cggtccggga ccgtatggcg ccaaaggaat gggtgaatcg ggcatcgtct cgatcgcacc
     2341 ggcgattggc aacgcgatct tccaagcgac cggagtgcgg atcagaactt tgccattgac
     2401 tcccgaacgg gtctggcgcg cgctcaatga tgcaaagcgc ggcatgtgag caaggagcta
     2461 cgcagtcgag atggaacgga atcaggtatt cgatagagtc agcgcaatca tcgaacgagc
     2521 gcgcgccgaa ctgatcgacc tctgtttaca gttagggaat atttcaagtc cccacggcca
     2581 ggagcgcgat ctcggagagg ccgtgctcga ctggttgaag cagtatcaga tccccggaca
     2641 actgcagttt atcacccaac gaagcgttaa cgccgttgca accatgacgg gcagcggtca
     2701 aggccccagt cttattttca atgcgcatat ggataccggt ccggagttgc cggctgacgc
     2761 aagcgaggat gctagaaagc ttgaaaccgc atgggttgaa ggcgagatgt tgtttggcaa
     2821 aggaatgatc aatgacaagg cgcagctgtg cgccttcatg atcgccatga gcgcgctcag
     2881 acgcgccggg gttcagctca acggagatct gaccctaacg gcggtggcgt cagaaaccgg
     2941 caacccctcc atcgatgagt ttcagggcat cgatttcccc ggcgagggat ttggcactaa
     3001 atggctcgtc gatcgcggcg cgattgccga ctatgctctg gtcggcgaaa cttcaggttt
     3061 cggtatcgtt caggccgaat gtggcgcggc atggtttaaa attcgggtca aaggcagaga
     3121 ggtctatacc ccgcgttacg agcggggctc gtcgatgcag gaaaatccaa atgcgtttgt
     3181 caaagccgct catgtgatcc aggcctttga ggcttgggcg atacagtacg agaaaagaga
     3241 aaccatcgag tttgccggcg ggaccatcat ccccaaagcg caaatcgtcg aggtgcgagg
     3301 cggcgccgga gtgggacggc caaacccgac ctgcgatatc ttcaccgacg tgagaattgc
     3361 acccggcaag aatccactta cggttcaacg ggagcttgcc gctgctatcg ccaaggtgaa
     3421 actggattgt gagatttcct tgttccagta ttctcgcggg catatcgcca agaatgccgg
     3481 acctttgatc gacgcggtta gatcggcgca ccgctatctc ttcggcgctg agccgccgca
     3541 accgccgtcg gcggaaacca gcatgtggcg cgacctgaac gttttcaacg aaatcggcat
     3601 tccgtccatc tgctatgggc cgccccggca acgagagcgt ctgagcggcg cccagaaccg
     3661 ggcgatgaaa attgacgatc tggtcgaagc gactaaggtt tacgcgctta gcgcgatgat
     3721 cttgtgtggc gtgcaatctt cttgagtcgg ggtccttagg agtgactgtg gtaaaatcgc
     3781 cgattcttca gccttgcaat ccttaattat tccattctaa gcagcgctaa atgaaattct
     3841 gcctccaatt aaaccctcaa gtttcggtcg ataaggcggc agcgcaactg atgccgactc
     3901 tgatcgagca ggtgcgcgtg gccgatgggg tgggctttga cgcattttcc atgggcgatc
     3961 actacaacat ccccggcttg cagcggctta atcaaattcc tgcgttggcg cggctctgcg
     4021 gcgaagccag ccgttgcgcc gtcggcaccg ccgtgatgct actgggcttg cgtcatccgg
     4081 tcaccgtcgc cagcgaactg gccagtctcg atgtcatcaa tgaaggcaaa tctttctttg
     4141 ccttcggcct gggctaccgt gatgatgaat tgaacgcttt taatctcacc aaggcgcagc
     4201 gctttcatcg gtttgtcgag ggagttgaga tcatcaagcg actctggacc gagaataatg
     4261 tgcattatga cggcacagca tttaagctca agaatgtcag cgtcgatccc aaaccgttgc
     4321 aaaagccgcg gccgccgatt tggatcgccg ccaattccga tgccgcggtt gcccgcgccg
     4381 cagccatcgg tgacggctgg atgatggggg ccgcattccg ccatcgatga attggagcgc
     4441 caggtggctc tctgccgtca aacgtggagc gctgccggaa agccaggttt gcctgacata
     4501 ccgataattc gtgaaacgtt tgtcgt
//
LOCUS       DASUTG010000900         5360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2066800, whole genome shotgun sequence.
ACCESSION   DASUTG010000900 DASUTG010000000
VERSION     DASUTG010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066800"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF089_20885"
     CDS             <1..223
                     /locus_tag="VF089_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176470.1"
                     /translation="AAEFIVGVSFAVCNFKDPSGKESLMGLGMNRWSWWRKSLGSKSI
                     RREALLDLLRHRYVREKQHAMRYRQHAEE"
     gene            303..584
                     /locus_tag="VF089_20890"
     CDS             303..584
                     /locus_tag="VF089_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176471.1"
                     /translation="MDLGEKVPEVIQIRVPREQNSWFYLSTDLEEERRCCGELQNDLP
                     ALYNEFPETAELLKRIESDGCKHRTKIRDMLARSDPQSFGLAEGRSIFS"
     gene            1156..3726
                     /locus_tag="VF089_20895"
     CDS             1156..3726
                     /locus_tag="VF089_20895"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX6176472.1"
                     /translation="MPALTEVEQTHSSATQRLLEESDLLLSLYRALIENPDLSSGLGA
                     ALEIVCQFTGWVVGTGWLPSDDETEIRLCSSWHADDPKLEEFIGACRQQSFPRDVGIP
                     GRVWHRGKDEWTRNLAAEPAEPFPLAPAAKIADVKAAFAVPITHDEHVDGVLVFHARE
                     AKEEDGRLTQVICTIATQLGFALQHKRLEEEILEQQALVRQSHENLEKQVEQRVRQQE
                     AVVDLGRQALGGAELSALMGSVTELVAKTLNVEFCKILELLPGNESLLLRVGVGWREG
                     LVGQTTVSAGTNSQAGYTLLRKEPVIVEDLRTEARFFGPPLLRDHAVVSGMSVIIGGS
                     DHPYGVLGAHTAKPRKFSQDDIHFLQAVANLLSQVIEHNRDLAEIRRNATWLERLIET
                     TQDAVVSIDRRGCIVLFNAAAERVFGYSADEIVGRKVNDLMAEPYATEHDGYIARYER
                     TGEPRAIGRIRTVEGRRKSGETFPLELSVTQVAAVESEEVHYAAFIRDISEVRRGQAW
                     LQSLIETTQDAVLSIDRQGRVVLFNPAAERIFGYPRNEVIGQKVNMLMAEPYATEHDE
                     YIARYERTGEPRAIGRIRTVTARRKSGELFPIELSVTEISVDRDVHYAAFIRDISEKT
                     KLQNQLMESGRLAAIGSTAAKIGHELANPLNGMSLTIQLLEQRLNRLTDGAGTQLAPT
                     VKRLKDEISRLQKLVGEFATISRKEKYVFRPVNLMQLIEDIVDVQAPHLAQGGIELKM
                     SLARDLPLISIDSDKMKQALLNLVKNAAEAMPQGGRISIDAYTTKDSVILEITDTGKG
                     IPLDINAFEPFVTTKKEGTGVGLVIVRQILTAHDGKISYRSKPGEGTTFRLELPQRFR
                     "
     gene            3866..5212
                     /locus_tag="VF089_20900"
     CDS             3866..5212
                     /locus_tag="VF089_20900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX6176473.1"
                     /translation="MTGTILVVEDQESARESLVDLLTAEGFEVHAAADGRAGIELVGQ
                     HDLDVVLTDVAMPHADGIAVLRHIREISPQTLVILMTAHATVETAVEAIRLGAQDYLL
                     KPLIFEDVLRKVQHLMNHRKLAWENQLLRREINRRFSPERPLGRSQAMQEIARLIQKV
                     APAPTTVLITGESGVGKEVVARTIHGHSRFSDNVFLPVNCSAIPENLLESQLFGHVKG
                     SFTGAVSSQEGLFQRARGGTIFLDEIGEMPLSLQPKLLRVLEEKVILPVGSTNPVKVN
                     VRILAATNRDLNAEVEAGRFRDDLFYRLNVFEINIPPLRKRLDDLPGLVEHLIQRHNQ
                     EMNATYKGVDSATMRILMSFQWKGNIRELDNVLERAMILGDGEWIKPADLPGHESDAG
                     EFNSEDNLSAALDLYEKSHIQRTLNKAAGDKMRAAELMGLSLSTLYRKLEKLAIEG"
BASE COUNT         1314 a         1383 c         1502 g         1161 t
ORIGIN      
        1 agccgcggaa tttatcgttg gcgtaagctt tgcggtttgc aacttcaaag atccttctgg
       61 gaaggagtca ctgatggggc ttgggatgaa tcgttggagt tggtggcgaa aatcgcttgg
      121 gtcgaaatcc atcaggcgcg aagccctatt agatcttctg cgccatcggt atgtaagaga
      181 aaaacagcac gcgatgcgtt atcgccagca tgctgaagaa tgagttaccc gcagtttcgg
      241 aatgtgctcc tcagcattgc gcagaggagg agttacacgc cccatgggtt gccgcgaaaa
      301 ttatggactt gggggaaaag gttccagagg tgattcagat ccgcgtaccc agggagcaga
      361 acagctggtt ttacctgagc actgatctcg aggaagagcg gcggtgctgc ggcgagttgc
      421 aaaacgactt gcccgccctt tacaacgagt ttccggagac ggcagaactc ctcaaacgta
      481 ttgagagtga cggctgcaag caccggacca agatccgcga catgctggcg cgtagcgatc
      541 cgcagtcatt tggcctggct gaaggaagat caatattttc ctaacttcat ctcatcatcc
      601 attcgcgctc tacccgtagc cgagcatcga ggtatcgctc tatcagttgg cgtcgatact
      661 ttattcgctg aagagccgca ggagttcgca atttcgccgc tcgcgatctc cacgtacgat
      721 cgtagccgtc acaagatggc ccaaggagct gttttgtgtc cgtgccggta cctagagcct
      781 gaattccttg cggctgctga atggacgtgg gtcaaacgtg cgaatgccga tcgtgccgct
      841 gctgcgcttt cgtcagcagc acgttcgcga tcaccaccag gaggcttaaa agcacgaaat
      901 agaaaaatac gctttcagtt accataatcc acctccgtta gagggttcac tcgatgtaag
      961 cgcaaagcaa atgccaaatt gtgctcaccg gcgggaataa acattacctg tgattacaat
     1021 gggttatctt gacagggtca gtctgctgaa atagacgtat tctcgaccat gacaaagcga
     1081 tctcgaaggc aagaatttca aactgagcat tcaccctatc cttcaagtgc aatttaaaca
     1141 gccggacagc gaccgatgcc tgcattgacc gaagtcgaac agactcacag tagcgcgact
     1201 caacggctgc tcgaggagtc cgatctactg ttaagtctct acagagcgct catcgagaat
     1261 cccgacctat cctccggcct tggcgccgct cttgaaatcg tgtgccaatt taccggttgg
     1321 gttgttggaa ccgggtggct tccttcagat gacgaaaccg agatcaggct ctgctcctcg
     1381 tggcatgcag acgatccaaa gctcgaggag ttcatcggtg cttgccggca gcagtcgttt
     1441 ccccgcgacg ttggcatccc aggccgggtt tggcaccgcg gaaaagatga gtggacgcgc
     1501 aatctagccg ctgagcctgc cgaaccgttc cctctggcac ctgcggcgaa aattgcggat
     1561 gtcaaagccg ctttcgcggt tccgatcact cacgatgagc acgttgatgg cgtgttggtg
     1621 ttccacgcgc gcgaggcgaa ggaggaagac ggacgtctaa cgcaagtcat ttgcaccatc
     1681 gcaacccagc taggattcgc gctgcagcac aaacggctcg aagaagaaat cttggagcaa
     1741 caggctctgg tccgccaaag ccatgaaaac ttggaaaaac aggttgagca gcgagtgcgc
     1801 cagcaggagg ccgtagtcga tctcggccgg caggcgttgg gcggagcgga gttatcggca
     1861 ctcatgggct cggttaccga gttagtcgcc aaaactttga atgtcgagtt ttgcaagata
     1921 ttggagcttc taccaggtaa tgagagcttg ctcttacgtg tgggtgttgg gtggagagaa
     1981 gggcttgtcg gtcagacaac cgtaagcgcc ggcacgaact cacaagccgg ctatacgttg
     2041 cttcgcaagg agccggtgat cgttgaagat ctccggacag aagcaagatt tttcgggccg
     2101 ccgctgttgc gcgatcatgc agtcgtcagc ggcatgagcg ttattatcgg aggcagcgat
     2161 cacccctacg gtgtccttgg agcgcatacc gcaaagccgc gaaagttttc gcaagacgac
     2221 attcatttct tgcaggccgt ggcgaacctg ctttcgcaag ttatcgagca caatcgcgac
     2281 ttggcagaga ttcgtcgcaa cgccacctgg ctcgaacgtc ttatcgaaac tactcaagac
     2341 gcggttgttt ccatcgacag gcgcggctgc atcgtcttgt tcaatgccgc cgctgaacga
     2401 gtattcggct acagcgcaga cgagattgtc ggacgcaagg tcaatgatct gatggcggag
     2461 ccctacgcca ccgagcacga cggttatatt gcccgctatg aacggaccgg tgagccacgc
     2521 gccattggcc gcatccgcac agtggagggc cggcgtaaaa gcggcgagac ctttcctctc
     2581 gagctttcag tgacgcaagt cgcggctgtt gaaagcgaag aagttcacta tgccgctttt
     2641 atccgggata tttctgaagt gagacggggc caagcttggc tgcaaagcct gatcgaaaca
     2701 acgcaagatg ccgtcctttc catcgatcgt caaggccggg tcgttctatt caatccggcg
     2761 gctgaacgga tattcggtta tccgcgcaac gaagttattg gacagaaggt caatatgctc
     2821 atggcggagc cgtatgctac agagcacgat gaatatatcg cgcgctacga aagaaccggt
     2881 gagccgcgcg cgatcggtag gatcagaacg gttacggcac ggcggaagag cggcgagctc
     2941 tttcctattg aactctcggt caccgaaata tccgtggata gagacgtcca ctatgccgcc
     3001 ttcattcgcg acatttcgga aaaaaccaag ctacagaacc agctgatgga aagcggacga
     3061 cttgccgcaa tcggcagcac tgcggccaag atcggccatg aactcgccaa tccgcttaat
     3121 ggaatgtccc tgacgattca gctccttgag caacgtctga ataggcttac tgatggcgcc
     3181 gggactcagc tggctccgac ggtaaagcga ctcaaggatg aaatctcgcg actgcaaaag
     3241 ttggtcggcg agttcgcaac aatctcgaga aaagaaaaat atgtctttcg accggtcaac
     3301 ctgatgcagc tgatcgaaga cattgttgac gtacaagcgc cgcatcttgc ccaaggtgga
     3361 atagagttaa agatgtccct ggctcgcgat ctgccgctca tcagcatcga cagcgataag
     3421 atgaagcagg ctctgctcaa cttggttaaa aacgccgccg aggcgatgcc tcaggggggc
     3481 aggatcagca ttgatgccta tacgacgaaa gactcagtga tactagaaat caccgatacc
     3541 ggaaaaggta ttcctttgga catcaatgcg tttgagcctt ttgtaactac caagaaagaa
     3601 gggactggag tagggctggt gatagtccgg caaatcctca ccgcccatga tggaaaaatc
     3661 tcctatcgaa gtaagcccgg ggagggaact accttcaggc tagagctgcc gcaaagattc
     3721 cgctaggatt cgttggcttg tgtattgctg ctcgccagag taacggaaat attaacggaa
     3781 ttagtccaag aatcagccta ctgcaattgc aacaactagg atttttctta accaaattcg
     3841 cagaactgcg aaagaggaaa gctacttgac cggaacaata ctagtggttg aggatcaaga
     3901 atccgcgcgt gagagtttgg tcgacctatt aacagcggaa gggtttgaag ttcatgccgc
     3961 agctgatggc agagcgggca ttgagctggt cggtcaacac gatctcgatg tggtgctcac
     4021 cgacgtggcc atgccgcacg cggatggcat cgccgtactt cggcacatac gtgagatttc
     4081 accgcagacg ctggtgatac tgatgaccgc tcatgccacc gtcgagaccg cggttgaagc
     4141 tatccgtctt ggcgctcagg actatttgct caagccgctt atttttgaag acgtgctgcg
     4201 caaagtccag catttgatga accatcgaaa actggcctgg gagaaccagc tgctgaggcg
     4261 agagatcaac agacgcttca gtcctgagcg gccgcttggg cgcagccagg caatgcagga
     4321 aatcgctcgt ctaatacaga aggtcgcgcc ggctccgacc accgttctaa ttacgggcga
     4381 gagtggcgtc ggcaaagaag tggttgcccg cacgattcac ggccacagcc ggttcagcga
     4441 taatgtgttt ctgccggtaa actgtagcgc aattccggaa aatcttctcg agagccagct
     4501 ttttggacat gttaagggct cctttaccgg tgcggtcagt tctcaagagg ggttgtttca
     4561 aagagcgcgc ggcggcacga tatttctcga cgagatcggt gaaatgcctc tgagcctcca
     4621 acccaagctg ctgcgtgtgc tagaggaaaa agtaattctt ccggtgggct cgacaaatcc
     4681 tgtcaaggtc aatgtgcgca tactggcagc aaccaatcgc gatctcaatg cggaagtgga
     4741 agcggggcgc tttcgtgacg atttgtttta tcgtctaaat gtattcgaga tcaatattcc
     4801 gccgttgcgc aagcggctcg atgatctgcc cgggttggtc gagcatctca tccagcgcca
     4861 caaccaggag atgaacgcga cctataaggg cgtcgatagc gcgaccatgc ggattcttat
     4921 gtcgtttcaa tggaaaggaa atatccgcga gctcgacaac gttctggaac gtgcgatgat
     4981 tctcggtgac ggcgaatgga tcaagccggc tgatctgcca ggccacgaat ccgatgccgg
     5041 ggaattcaac tcagaggata atctcagtgc agcgcttgat ctctatgaga agagccacat
     5101 ccagcgcacc ttaaacaagg ccgctggaga caagatgcgc gccgcagagc tcatgggact
     5161 gagtctgtcg acactctacc gaaaacttga gaagctggcg atcgagggct gaacagactt
     5221 catggctgga tcatcttagc ggcgccggtg tgtgctccta ttcgcgcata accatcgaat
     5281 gaattactgt gccacagtcg cgtccgtaag ctcgtctgaa gtacacgata aaatctaact
     5341 tcgtacgcgc agactccagc
//
LOCUS       DASUTG010000901         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2216598, whole genome shotgun sequence.
ACCESSION   DASUTG010000901 DASUTG010000000
VERSION     DASUTG010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216598"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF089_20905"
     CDS             <1..391
                     /locus_tag="VF089_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176474.1"
                     /translation="AWFAGPLKWFVQNRQLRQLASNGRRFLFTFNPKTRQSKYVTFLA
                     NGQIGEGQNQNEHAWRIRRGLLEIFAADGAIYSRFRHDKVSGDLKHTNDADLRSIHGQ
                     YLEPSLVRVTKPAEQAVAPDRPKMGSG"
     gene            complement(779..1129)
                     /locus_tag="VF089_20910"
     CDS             complement(779..1129)
                     /locus_tag="VF089_20910"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX6176475.1"
                     /translation="MSSKKVLQKWQTSAAALSLLAVLSASCGAVHNPSLDRARDAYER
                     VRRDPGVVGRAAVALEKTRLTLEEAERVWATEKDPSEVEHLVYLTEKRAEIALATARR
                     RIAADEIQQLRPQR"
     gene            complement(1126..1602)
                     /locus_tag="VF089_20915"
     CDS             complement(1126..1602)
                     /locus_tag="VF089_20915"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX6176476.1"
                     /translation="MGTIFYIKAAMIRRAKTHLPFRAFSYMIFASALPFFGCSILPLN
                     SGRAAVPAVGAFSKAELSLRTAAEARADELAPMDLQRAKDKLENARRAMAAGRNTEAR
                     RLAEAADVEAQLAQAKAEAEIMRLAADYIRRRGEGLREIEGAARPGSAMETPKGKR"
     gene            2006..>2312
                     /locus_tag="VF089_20920"
     CDS             2006..>2312
                     /locus_tag="VF089_20920"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6176477.1"
                     /translation="MFPYRDENETQRTAIVTLAMIALNVFAWLFVQGAGTTLALARSV
                     CELGLIPGELTGAIAPGTRFPIGEGLACVTDPGRQFSHVLTHMFLHGSWMHLLGNMWF
                     "
BASE COUNT          504 a          626 c          642 g          540 t
ORIGIN      
        1 ggcgtggttc gcgggtccac tgaaatggtt tgtgcaaaac cgacagttgc gacagctggc
       61 ttcaaacggt cgcaggtttc tgttcacctt caatccgaaa actaggcagt cgaagtacgt
      121 caccttccta gccaatgggc aaattggcga aggacagaat cagaacgaac acgcttggag
      181 aatacggcga ggattgctgg agatctttgc tgctgacgga gcgatctata gccgctttcg
      241 tcacgacaaa gtgagtggcg atctcaaaca cacaaacgac gccgatctcc gttcgataca
      301 tgggcagtat ctcgagccgt ctctggtgcg tgtcacgaag ccggccgaac aagccgttgc
      361 acccgatcgc ccgaagatgg gctccggctg aacggtaacg tacggacagg tagagcatga
      421 cagaccgttg gaacggggat aagcaagacc tcgtgaagtg gggtgtcctc ctgaacctcg
      481 ccacggacca tgctgccggc aggatccttc aggtcaccta tttccggccg agcgagtggc
      541 cgccagttga catcgacggt aaggacgctc gattccggaa atggtgctgc gacacttccg
      601 ggatgtacgc accgtcactg cgatctcaac cacaccgcgc atcgaggtcg tcgacacgcc
      661 gtaagatgac tactttcttt acgagctacc gttctgaggt gggtcataca ggtagagcat
      721 aggcttttta gcgtgttcgt tttaacccct gcgccttgat cctcggcacg ccagcatctt
      781 aacgttgcgg tctgagctgc tggatttcgt cggccgctat cctgcgtcgt gccgtagcga
      841 gcgcgatctc ggctcttttc tcggtgaggt aaaccagatg ctcaacttcg ctgggatcct
      901 tctcagtggc ccatacacgt tcggcttctt cgagagtcaa acgggttttt tccaaagcaa
      961 ccgcggcgcg cccgacgact ccaggatctc gccgaactct ttcataagcg tctcgtgcac
     1021 ggtccaagct gggattatgc actgcgccgc atgaggcgga aagaactgcg agcaacgaca
     1081 gcgctgctgc gcttgtttgc cacttttgca aaactttttt cgaactcatc gtttaccttt
     1141 cggcgtttcc atagccgatc cgggtcttgc ggcgccttca atttcacgta atccctcgcc
     1201 acgccggcga atgtaatccg cagccaagcg catgatctcg gcttcagcct ttgcttgggc
     1261 gagctgcgct tctacatcag cggcttcggc gagacggcga gcttcggtat tcctccccgc
     1321 cgccatcgct cttctggcgt tttccaattt gtccttagct ctctgcaaat ccatcggagc
     1381 gagttcgtca gcccgggcct cggcggccgt gcgcaagctg agttctgcct tggaaaaggc
     1441 gccaactgcc ggcacagccg cgcgccccga gttcaaaggt aagatgctgc agccgaaaaa
     1501 aggcaatgcc gatgcaaaga tcatatagct aaaggcgcgg aagggcaggt gcgtcttcgc
     1561 gcggcggatc atagctgctt tgatgtagaa gatcgtgccc atcggctttc ctccaggttg
     1621 tttgatacgc caagcacgag attgctgaag ctaaaaggca caaagtcgct gatgaaattg
     1681 tataactcta aaaattaact acaagactaa acggcggtta acatcgaatc acttgcgata
     1741 aggaaatcga gaagggacgg ctttaatgcg tctgcggacg ttttatgatc atgaacggtt
     1801 tacctatgaa cccgcaaact tttgcagtag tacggagaat tccgggttcg gagcattttc
     1861 cgggccttca ctgtacgcaa cactagcgca agtatattcc gccagatatg ttgaatgaga
     1921 atgcttgcaa tcattgcaat gcgagcggtg tcgctaaatt ctacgctcct ttggagttag
     1981 attagcggaa gagagaggag ggcgcatgtt tccttatcgc gatgaaaacg aaacacaacg
     2041 tacagccatc gtaaccttgg cgatgattgc gctaaatgtg tttgcctggc tcttcgtgca
     2101 aggggccggt accacgttgg cgctggcgcg ctcggtgtgc gagctcgggc taataccggg
     2161 agaactgacg ggtgcgatcg cgccgggtac gcgatttccc ataggcgagg ggcttgcctg
     2221 cgttactgat cctggccgtc agttctccca tgtcctgacc cacatgttct tgcatggctc
     2281 gtggatgcat cttctgggga acatgtggtt tt
//
LOCUS       DASUTG010000902        10125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1771040, whole genome shotgun sequence.
ACCESSION   DASUTG010000902 DASUTG010000000
VERSION     DASUTG010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771040"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1085
                     /locus_tag="VF089_20925"
     CDS             87..1085
                     /locus_tag="VF089_20925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6176478.1"
                     /translation="MIGKLLVVLTFILCAVSAPQAQTLDEVYKQALTEGGTLNFYATL
                     AQVNAASILPAFEKRFPGIRVNHVDATSDQLAARVIAESRGGKVVADVFQTLLDGMIR
                     VQNQGLFLEKLYPEADEYPAELKGTTWVATDLIYIVAAWNTTKVKKQEEPKQFDDFAD
                     PRWKNQLIAEPRDFQLLLGLAKHKFKSDEKAVALLKRIAENNVQFHKGHSQLAELLVA
                     GQAAACVTCYSHHYPSRKRKGAPVDYMLTEGTGDIDATAIMKNAPHPNTALLFARWTA
                     SQEGQKIYAQGGRLPAHPKVEPVEKTRPTKVYPLGEQDVKDYPKYEKMWKEIFGLR"
     gene            1736..1960
                     /locus_tag="VF089_20930"
     CDS             1736..1960
                     /locus_tag="VF089_20930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX6176479.1"
                     /translation="MHQATTQFSRRVDLAANGEEIIIAKSGRPVARLVPYQGKGVMRR
                     PGTMRGKIRIKKNFDEPFPKDLLASFAGKS"
     gene            2019..2729
                     /locus_tag="VF089_20935"
     CDS             2019..2729
                     /locus_tag="VF089_20935"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176480.1"
                     /translation="MKLTGLISPNFSEDTLEDVYKVFPPGIRIEGRTLKVEKYTDDEF
                     RRAELMLADLVRDLARHPLDFLMVTGELFLSYKGPGSDSEILDLVKSITAIPASTVLT
                     AVVRGCHALELKRIVMASPFPEDQDERLVKFLDHYGIEVVAFRGLGCPNADVIWELPP
                     ETGYELAAALLNEYQDVDGIYMPCNKWRVISVIERIEKDHGKPVVSNTQAWVWEALRL
                     MNMAKPIPGYGRLLSEFK"
     gene            complement(2862..3968)
                     /gene="tcuB"
                     /locus_tag="VF089_20940"
     CDS             complement(2862..3968)
                     /gene="tcuB"
                     /locus_tag="VF089_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEX6176481.1"
                     /translation="MAELDLFKDGDRQMTICNACRYCEGYCAVFPAMELRRTFTKADL
                     TYLANLCFDCRDCYYACQYAPPHEFGVNIPKLMAGLRTETYRRYSWPAVFSALFKRNG
                     LAVGLISTASLLVILVLVLAFMGSDILLATHLGEGAFYRVIPFTAMAVPPMIIVVYAL
                     VVLLAGARRFWRESGGELSEVIDPRALWRATQDAFGLAYMKGGGAGCNYPDASFSESR
                     RWFHHLVFYGFLLALASTTIAALYHHFLDWQAPYPLLSWPVVLGTTGGIAMVIGTSGM
                     LYLKWRSDRNPATESMLNMDVAFLVLLLLTSLTGLLLLALRETSAMGTLLVVHLGIVA
                     GMFITAPYGKFAHVVYRYAALVRYAIEQRRIDAR"
     gene            complement(3955..5352)
                     /gene="tcuA"
                     /locus_tag="VF089_20945"
     CDS             complement(3955..5352)
                     /gene="tcuA"
                     /locus_tag="VF089_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX6176482.1"
                     /translation="MGNSGALFDVLVVGGGNAGMCAAIMARRGGARVLVLESATQDFR
                     GGNSRHTRDIRYMHKAATDYVTGPYEEDEFWEDLRQVTGGETNEALARLTIRGSEELG
                     DWMPANGVRWQKPLRGTLHLTRSNLFMLGGGKAMMNAYYDTASRLGVEIAYESEVGEL
                     AIRDGEFLSAAVFRNGASQEIHARAIVVAAGGFEANIPWLKEYWGDAADNFIIRGTRH
                     NQGRMLKELLTHGAKPVGNQRGCHAVALDARAPKFDGGIVTRLDSPPFGVVVNKDVKR
                     FYDEGEDFWPKRYAIWGGLIAQQRDQIAYSIIDAKALPHFMPSVFPPVEACSIAELAV
                     AFGLDPARLASAVDEFNEAVRPGTFNPGNLDDCRTEGIDPPKSHWARRIDTPPYFGYP
                     LRPGITFTYLGVTVNEQARVIMQDERPAKNIFAAGEIMAGNILGKGYLAGFGLTIGSV
                     FGRIAGREAARYGGT"
     gene            complement(5352..7283)
                     /locus_tag="VF089_20950"
     CDS             complement(5352..7283)
                     /locus_tag="VF089_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6176483.1"
                     /translation="MWDAALIAFERFANPLHMGMLLAGVLAGTVIGILPGLGGIACVA
                     ILLPFIYTLEVHTAMILLVGSLAVVHTSDTITSVLIGAPGSAAAAATVLDGHPLAKQG
                     QAARALSAAFLSSLIGGLIGAVFLTFSLPIARPLVLLFGSPELLMLCVLGLSFAGFLT
                     GGAPLKGGLAACLGLLLGSVGSAPADAVNRYTFDQLYLLDGIPLVAVALGVFGIAEVI
                     DLLAKGGRIAERIGLGHGWLMGVKDVMQHWGIVVRGSLIGVWAGILPGIGATAGSWMA
                     YGHVVAMARDRKGFGKGDIRGVIGPESANNSVEAGDFIPTLLFGVPGGAPAAIMLGAL
                     FFYGIQPGPRMVQENLDLIFTIIWSFAIANTVGAGLCFLLSPALARLTWVPFALLAPA
                     VIVTIFFGAFQSSQQFGDIYAMLGLGLLGWLMKQLGWPRAPFLVGFVLTKPTEQYLWL
                     SISRYGFDWLLRPGVIVLGLLLLASIIWIVRQRRSGNDLSAEESAEGAVVFGKLPSVL
                     FTILVLLVVGAALYEAQDFPHLGAIFPMAATIPAIFMAAGQIFLEVHTTREVPAIETR
                     RKTKLALGYFVSLLLYLLLVLLFGFGAATAVFTFVFLYGWVRTGWTHALIYTGAVVGV
                     TLLMSWLLNLYWPEGMLFG"
     gene            complement(7398..8498)
                     /locus_tag="VF089_20955"
     CDS             complement(7398..8498)
                     /locus_tag="VF089_20955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176484.1"
                     /translation="MKKIRFKTSLPIGRWLAFFGLSLALTGPALGAAPYYEGKTVEVI
                     IPFPVAGGTDIWIRTIAPYLEKHIPGNPKFNFRNIGGGRGIPGMMEFALKAKPDGLML
                     LVSSATNYFPVLLGDKAAKYDLRQWKPLLVNPVGGVMYASPASGIKRVEDLAKAKDLI
                     YGGISAIGLDLIPLISFELLDLDAKGVLGFKGRGEARIAFERGETNIDYQTTPAYNAT
                     VVPLIKEGKAIPLMSFGQLDDKGNIIRDPAVPDLPTVPEVYEKLKGKKPSGRFWETYK
                     VFMPSAFAVQKILWTKGDAAADVGRVFYAAADLLEKDKEFQTKTDKVLGGYPLLRGDR
                     LEKTIQEAFQIDAETQRFVKEWVSKKYRVKFD"
     gene            8920..>10125
                     /locus_tag="VF089_20960"
     CDS             8920..>10125
                     /locus_tag="VF089_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6176485.1"
                     /translation="MARVTGSHLICKALQLEGVRNIFALAGDHILPVLDVMADLDFRI
                     IDTRHEQAAVHMADGWSRITEQPGVCMYTTPGFANAIPGLTNAMHTEAPVISIAGCAD
                     AHDLGRGAQQEIDQIAMAGPMCKGSFMVHDPRRIPEFIARAMRLAFSGRRGPVHLTIP
                     IDVQERMVEEDDVGFTAPDGYRPRAALLGDPELVRQAVALLRQAQRPLVIAGSAAGYT
                     LSGEAMQRFLEATRLPLVTEEQSRGLVPDDHPYVFGYFERGLNRAAAKLRDADVVVLL
                     GRKQDFIIGFCRPPHVAANAKIIQIDPSPLEIGRNRGVAVGISGDVSSVLEQMTREAA
                     NYSWEDLPWLEELRAARSAQAEWAEDLARSQTPMHALFVHKTLKTILRADDCLVFDGG
                     DFCHFGRSFL"
BASE COUNT         2359 a         2898 c         2896 g         1972 t
ORIGIN      
        1 agggtggtac tacagccaac cgcatgttat cggtcgggcg aaacgggact tgagataaag
       61 ccgcaataac tcgtgacgga ggaactatga tcggaaagct gttggtcgtc ctgactttta
      121 tcctttgcgc agtaagcgcg ccgcaagccc agacactcga tgaagtttac aagcaggctt
      181 tgacagaagg cggcacgctc aatttctacg cgaccttggc gcaggttaac gccgctagca
      241 ttcttcccgc cttcgaaaag cgttttccag gcatcagggt caatcacgtt gacgcaacgt
      301 cggatcagct tgccgcccgg gtcattgccg aatcgcgcgg cggtaaagtt gttgcggatg
      361 tttttcagac gctactggac ggcatgattc gcgtccaaaa tcagggcctg ttcctcgaga
      421 aactgtatcc tgaagccgat gaatatccag cggagctcaa aggaacaacc tgggtggcta
      481 ccgatctgat ttacatcgtg gccgcctgga atacgacgaa agttaagaag caggaagagc
      541 cgaaacagtt tgacgatttt gccgatccac gttggaagaa tcaactcatc gccgagcctc
      601 gagatttcca gttgttgctc ggtctggcca agcacaaatt taaaagcgac gagaaggcgg
      661 ttgccttgct caaacgaatc gccgaaaaca acgtccagtt ccacaaaggc cactcgcaac
      721 tggccgagtt gctcgtcgcc gggcaagccg cggcctgcgt tacctgttac tcgcatcatt
      781 atccgagccg gaaaagaaag ggcgcgccgg tggattacat gctaaccgaa ggaaccgggg
      841 acatcgacgc caccgcgatc atgaagaatg ccccgcatcc aaatacggct ttgctttttg
      901 cgcgttggac cgcaagccag gaaggacaaa aaatctacgc gcaaggcggc cgcctgccgg
      961 ctcatccgaa ggtcgaaccg gtagaaaaaa cccgccccac aaaggtctac ccgttaggcg
     1021 agcaagatgt caaagactat cccaagtacg aaaagatgtg gaaggagatc ttcgggctgc
     1081 gttaactgac gaggggactc tcaaaactga tgtctgatca ttgacgccac ggacggcact
     1141 cccagatctt catcatcctg ccccaatgtc ttcgatggct cgatgtattg actcactaaa
     1201 agcttccggc ttgggcaaca ttgcggcgag acacccgacg atctccacat gaccaagtac
     1261 ttattcaaca atattcgaac caactgcaag acctgcgcaa gatttcggaa cccatgtgaa
     1321 tcctttgacg cgaagcattc aaagaccggt tctccgagtg gcaggggtcc ttataggtga
     1381 gaacggctgt tgatgatctt cattgaggat ttgcgcgaaa caacccgctg ggtgcctgcg
     1441 caacgtacaa tagaagccgt gcttcgttat aatcaaaccc gagcacttag taggaattca
     1501 caaaatgttc tagccgatcg aggtggaccg cgccaaagaa gatttaacat gtgagtggag
     1561 gttggtcgtc gcgtcatttg aaagcaagct ggccatttgc agtgaagtac cgcgcttgtc
     1621 aggtggcata gggtcgtgtc tgataatcac tcattcttct ctccgactcg ataggcctca
     1681 cgattgataa ttctggggag acgggattaa gctttttggt tatgaaaaag tcgatattca
     1741 tcaggcaacg acgcagtttt cccggcgcgt cgatttggct gcgaacggag aagagatcat
     1801 catcgccaaa tcgggaagac cggtcgcccg gttggttccc taccagggaa aaggcgtcat
     1861 gcgcaggccg ggaaccatgc gcggtaaaat tcgcatcaag aagaacttcg acgagccgtt
     1921 tccgaaagat ctgctggctt cgttcgcagg gaagtcgtaa aagtgcgtct gctctagttt
     1981 agcagagtgt ctcgcaaata aaccgatcgg agatctggat gaagctcacc ggactgatat
     2041 cgcccaattt cagcgaggat actctagaag atgtttataa agtcttcccg ccggggatac
     2101 gcatcgaagg gaggacgtta aaagtcgaaa aatacaccga tgacgagttt cgccgagccg
     2161 agctgatgct cgccgatctc gtgcgcgatt tggcgcgcca tccgctcgac tttctcatgg
     2221 tcacgggcga gttgtttcta tcctacaaag ggccgggctc ggattcggaa atcctagatc
     2281 tggtcaagag catcactgcc attcccgctt ccacggtgct gaccgcggtg gtacgcggct
     2341 gccacgcgct tgaactcaag cgcatcgtca tggcttcgcc gtttcccgag gatcaggatg
     2401 agcgcctggt aaaatttctc gaccattacg gtatcgaggt cgttgcgttt cgcggcctcg
     2461 gctgtcccaa tgcagacgtg atctgggagt tgccgccgga gaccgggtac gagttggcgg
     2521 cggcgctgtt gaacgaatat caggacgtgg acggcatcta catgccgtgc aacaagtggc
     2581 gcgttatctc ggtcatcgag cgcatcgaaa aagatcatgg caagccggtg gtgagcaaca
     2641 cgcaggcgtg ggtttgggag gcgctgcggc tgatgaatat ggcgaagccc atacccggtt
     2701 acggacggct cttgagcgaa ttcaagtgat caagccgagg gcctttcgga atgggctaga
     2761 gaaacgcggc catgacctcg cgcaatgtcg gcaggaagag tcaacaggcc cctgcgacca
     2821 aaaaccttgc tcctcgggcg ctaacgttgc gaatacaccg tctagcgggc gtcgattcga
     2881 cgctgctcga tggcgtagcg cacgagtgct gcgtagcggt agactacgtg agcaaacttc
     2941 ccgtagggtg cggtgatgaa catgccggcg acgattccga gatgaacgac caggagcgtt
     3001 cccatcgcgg atgtttcgcg caacgcgagc aacaggagtc cggtcaggct ggtgagaagc
     3061 agcaacacta agaaggccac gtccatgttc aacatgcttt cagtcgcagg attgcgatcg
     3121 ctgcgccatt ttagataaag catgccgctg gtgccgatca ccatggcgat gccgccggtc
     3181 gttcccagca caaccggcca gctgagcagc ggatagggtg cctgccagtc gagaaagtga
     3241 tggtagagtg cggcaatggt ggtcgaggca agggcgagca agaagccgta aaaaaccaaa
     3301 tggtgaaacc atcgccgcga ctccgagaaa ctcgcgtccg gatagttgca gccggcgccg
     3361 ccgcccttca tataggcgag tccgaaggcg tcttgagtgg cgcgccacag ggcgcgcggg
     3421 tcgatcacct cggataattc tcccccgctc tcgcgccaaa atcgccgggc cccggccaaa
     3481 agaaccacca gagcatagac cacgataatc atcggcggca cggccatggc ggtgaaggga
     3541 atcacgcgat aaaaagcgcc ttcacctaaa tgggtcgcca acaaaatatc gcttcccatg
     3601 aacgccaaca ccaggacgag tatgaccagc agcgaggccg tgctgatcaa gcccacggcc
     3661 aagccgttgc gcttgaagag agcggagaaa acagccggcc aactataacg gcgataggtc
     3721 tccgtgcgta gaccggccat gagcttggga atgttaacgc cgaactcgtg cggcggcgca
     3781 tactggcagg cgtaatagca gtcgcggcaa tcgaagcaga gattcgcaag atacgtaaga
     3841 tcggccttgg tgaaagtccg gcgcagttcc atcgccggaa aaaccgcgca ataaccctcg
     3901 cagtaacggc atgcattgca gatcgtcatc tgccgatcgc catctttgaa gagatcaagt
     3961 tccgccatac cgcgccgcct ccctgccggc gattctaccg aagaccgaac ctatggttag
     4021 accgaatccc gccagataac cttttcccag tatattgccg gccataattt cccccgccgc
     4081 aaaaatattc tttgccggcc gctcgtcctg catgatgacg cgcgcctgct cattcactgt
     4141 gacaccgaga taagtgaaag taatgccggg acgcagcggg tagccgaagt atggcggcgt
     4201 atcgatgcgc cgcgcccagt ggctcttcgg cggatcgatg ccctcggtgc ggcagtcatc
     4261 cagattgccg ggattgaatg tgcccggccg gaccgcctcg ttgaattcgt caaccgcgga
     4321 cgcaagccgc gccgggtcca gaccgaaggc gacggccagc tctgcaatgg agcacgcctc
     4381 tactggcgga aataccgagg gcatgaaatg aggcagcgcc tttgcgtcga tgatcgaata
     4441 ggcgatttga tcccgttgct gcgcgatcag accgccccag atcgcgtatc gcttcggcca
     4501 gaagtcttcg ccttcgtcgt agaagcgttt cacatcctta ttgaccacga cgccgaacgg
     4561 cgggctgtcg aggcgggtca cgatgccgcc gtcgaacttc ggcgcgcgcg cgtcgagcgc
     4621 cacggcgtgg cagccgcgct gattgccgac cggtttggcg ccgtgcgtga gcagttcctt
     4681 caacatccgt ccctgattat gcctggtgcc gcggataata aaattgtcgg cggcgtcgcc
     4741 ccagtactcc ttcagccaag gaatgttggc ctcgaacccg ccggctgcaa cgacgatcgc
     4801 cctggcgtga atttcttgcg acgcgccgtt gcgaaaaacg gccgccgaga ggaattcgcc
     4861 gtcgcgaatg gccaactcac cgacttccga ctcgtaggcg atctctactc caagcctcga
     4921 agcggtgtca tagtaggcgt tcatcatcgc tttgccgccg ccgagcatga agagattgct
     4981 ccgcgtcaag tgcagggtgc cgcgcagcgg tttttgccag cgcacgccat tggcaggcat
     5041 ccagtcgcca agctcttccg agcctcgaat tgtcagccgg gcaagcgctt cgttggtctc
     5101 gccgccggtc acttgccgca ggtcttccca gaattcgtct tcctcgtacg ggccggtgac
     5161 gtaatcggtg gccgctttgt gcatgtagcg gatatcgcgc gtgtggcggc tgttgccgcc
     5221 gcgaaagtct tgagtcgcgc tttcgagcac gagcacccgc gcaccgccgc gccgcgccat
     5281 gatggcggcg cacattcccg cgttgccgcc gccaaccacc agaacatcaa agagcgcgcc
     5341 tgaatttccc attagccaaa gagcatgcct tccggccagt aaaggttgag cagccaactc
     5401 atgagcagcg tcacgccaac gacggcgcct gtgtaaatca gtgcatgagt ccagccggtg
     5461 cgcacccaac cgtagagaaa aacaaaagtg aaaactgcag tggcggcgcc gaagccgaag
     5521 agtaaaacca gcaagagata caagaggagt gaaacaaaat acccgagcgc caatttagtt
     5581 tttctccgtg tctcgatcgc cggcacttcg cgagtggtgt gcacttcaag aaagatctga
     5641 cctgcagcca tgaaaattgc gggaatcgtc gccgccatcg gaaagattgc gcccaggtgg
     5701 ggaaagtctt gcgcctcata aagcgccgcg cctacgacca gcaaaaccag aatcgtgaaa
     5761 agaaccgacg gtagtttccc aaagacaacg gcgccctcgg cggattcttc ggccgaaagg
     5821 tcattgccgc tgcgtctctg gcggactatc cagatgatgg acgccagcag caagagaccg
     5881 agaacgatca cgcccggacg cagcagccag tcgaagccgt agcggctgat cgagagccaa
     5941 agatactgtt cggtcggctt ggtcaaaacg aagccgacga gaaaaggcgc gcggggccat
     6001 cctaactgct tcatgagcca accgagcaac cccaagccca gcatggcata gatatcgccg
     6061 aactgctggg agctctgaaa tgcgccaaaa aaaatcgtaa cgatcacggc cggagccagc
     6121 agagcgaacg gaacccacgt cagccgcgcc agggccgggc tcaacagaaa gcatagcccg
     6181 gcaccgacag tattggcgat ggcaaaactc cagataatgg taaaaattag atcaaggttt
     6241 tcctgaacca tgcgcgggcc aggttgaatg ccataaaaaa acaacgcgcc gagcatgatc
     6301 gcggcgggag cgccgccggg tacgccgaac agcaaggtcg gaataaaatc gccggcttcg
     6361 acggaattgt tggcgctttc cgggccgatc acgccgcgaa tatcgccctt gccgaagcct
     6421 ttccggtcgc gggccatggc gaccacatgt ccataggcca tccaggatcc cgccgtagcg
     6481 ccaatgcccg gaagaattcc cgcccacacg ccgatcagtg atccgcgcac cacaatcccc
     6541 caatgttgca tcacgtcttt gacacccatg agccagccat gacccaggcc gattcgttcg
     6601 gcgatccgcc cgcctttggc caatagatcg atcacttcgg cgatgccaaa aacacccagt
     6661 gctacagcga ccaggggaat gccgtcgagc aggtaaagct ggtcgaaggt gtagcgattg
     6721 accgcatccg cgggagcgga gccgacactg cccaacaaga gtcccaggca agccgccagc
     6781 ccgcctttaa gcggtgcgcc gccggtgaga aagccggcaa aggagagacc aagcacgcac
     6841 agcatcaaca actcgggaga gccgaagagc agaacgagag gccgcgcgat gggcagggag
     6901 aaggtcaaaa aaacagcgcc gataaggccg ccaatcaatg acgacaaaaa ggccgcgctc
     6961 agcgccctgg cggcctgacc ttgcttggcc agtggatgac cgtccagaac cgttgcggcc
     7021 gcagccgccg aaccaggtgc gccgatcagc accgaagtga ttgtatcgga agtatggacc
     7081 accgccagtg atccgaccag caggatcatc gccgtgtgca cctcgagcgt atagatgaaa
     7141 ggaaggagga tggcgacgca ggcaataccg cccaagccgg gcaggatgcc aatgacggtg
     7201 ccggccagca cgccggccag cagcatgccc atatgcagag gattggcaaa cctctcgaaa
     7261 gctattaggg cggcatccca catgggagag cgatcaacaa cacgaacaga ttggggtggt
     7321 ggagtatgga agtggtggag tgttcggtct taaagccatt actccaccac tccagtgctc
     7381 cgccatgtat cagcttgcta gtcgaatttg acgcggtact tcttactcac ccactctttc
     7441 acgaaacgct gcgtctccgc atcaatctgg aaggcttctt ggatggtttt ttccaggcga
     7501 tcgccgcgca gcagcggata gccgcccaga accttgtccg tcttggtttg aaactcttta
     7561 tctttctcga gcaggtcggc agccgcatag aaaacgcgcc ccacgtccgc cgcggcatcg
     7621 cctttggtcc agagaatttt ctgcaccgcg aaggcggagg gcataaaaac cttgtaagtc
     7681 tcccagaatc tgcccgacgg ctttttaccc ttgagcttct catagacttc gggaaccgtc
     7741 ggcagatcgg gcacagcggg atcgcggatg atattgcctt tatcatcaag ctggccaaag
     7801 ctcataagcg gtatggcttt gccttctttg attagcggca ccacagtagc gttgtaagcg
     7861 ggcgtcgtct ggtagtcgat gttggtctcg ccgcgttcaa atgcaatgcg cgcttcgccc
     7921 ctgcctttga agccgagcac gcctttggca tcgagatcca gcagctcaaa gctgatgagc
     7981 gggatcagat ccaggcctat agcgctgatg ccaccataaa tcagatcttt ggctttggcg
     8041 agatcctcta ctcgcttgat tcccgaggct ggagaagcat acattacccc gcccacggga
     8101 ttgactagca gcggcttcca ctgacgaagg tcgtacttcg ccgccttgtc acccaagagc
     8161 accggaaagt aattggttgc cgagcttacc agcagcatga ggccatcggg tttggccttg
     8221 agggcaaact ccatcatgcc cgggatgccc cggccgccgc caatgttgcg aaaattgaat
     8281 ttgggattgc cgggaatatg tttttccaga tacggcgcaa tggtgcggat ccagatgtcg
     8341 gtgccgccgg cgacgggaaa tggaatgatg acttcgaccg ttttcccctc atagtaggga
     8401 gcggcgccga gcgcgggacc ggtaagcgcc aatgacaacc caaagaacgc cagccatcgg
     8461 ccgataggta gcgaagtttt gaaacggatc tttttcatgg tacccctcca aaacaagttc
     8521 ttctcccata ctgtcatgca tctcgttatg tcaagggagc agaagataac tcatgattgc
     8581 aatcggagga tctgagctga gtacttcttc aacttagccg ccgccttatg ctgcggagac
     8641 tttaacccgt tatgaaagtc cgtcgcttcc cgcatgctct tcgcgggagc cagtggacgt
     8701 tccaagacga tttcgaaaaa tctagaatgg aaatttctgc gggttatgca ttagtgcgca
     8761 aattctccct ggaccgaagc accgattgcc aattctaatc atcgtggatt gacggcgctg
     8821 ttatgctgta ctaaacgaga gtcgttcaag gcattcgtag cgccatttgc gcatgatgga
     8881 acagagcaca ttcgctcact gtagacacag gaggcggtaa tggccagagt caccggaagt
     8941 cacctcatct gcaaggctct ccaactcgaa ggggtgagaa atatcttcgc cctggccggc
     9001 gatcacattc tgccggtttt ggatgtcatg gcggatctgg actttcgcat tatcgatacc
     9061 cgccatgaac aagcagcggt ccatatggcg gatggctgga gccgcatcac cgaacaaccc
     9121 ggcgtttgca tgtacaccac gcccggtttc gccaatgcga ttcccggcct cactaacgcc
     9181 atgcataccg aagcgccggt tatctccata gccgggtgcg ccgatgcgca cgacctgggg
     9241 cgcggagccc agcaggaaat cgatcagatc gccatggcgg gcccgatgtg caaaggctcg
     9301 ttcatggtcc acgacccgcg aagaattcct gaattcatcg cgcgcgccat gcgcctggct
     9361 ttcagtgggc ggcgcgggcc ggttcatctg acgattccaa tcgacgttca agagcgaatg
     9421 gtcgaagaag atgacgttgg attcaccgcg cccgatggct accggcccag agcagcgctg
     9481 ttaggcgatc cggaactagt gcggcaggct gtcgctctct tgcgccaggc gcagagaccg
     9541 ctggtgatcg ccggcagcgc cgccggttac acgctctcgg gtgaggcaat gcagcgcttt
     9601 ctcgaagcca cccgcctgcc gctggtcacg gaagagcagt cacgcggctt ggttccggac
     9661 gaccatcctt acgtctttgg ttatttcgag cgcggcttga atcgcgccgc agcgaaactc
     9721 cgcgacgccg acgtggtggt gctgctcggc cgcaagcaag atttcatcat cggcttctgc
     9781 cgtccaccgc acgtcgccgc aaacgccaaa atcatccaga tcgatccttc gccgttggag
     9841 attggccgca atcgaggcgt tgctgtcggt atcagcggcg atgtcagcag cgtcctggag
     9901 cagatgacca gggaggcggc aaattatagt tgggaagatc taccctggtt ggaggaactg
     9961 cgcgcggctc gatcagcgca ggccgagtgg gccgaggatc tggcccggtc gcaaacgccg
    10021 atgcatgcct tgttcgtcca taagacgctc aagacgattc tgcgcgcgga cgattgcctg
    10081 gtcttcgatg gcggcgactt ctgtcacttc ggacgatcat ttctc
//
LOCUS       DASUTG010000903         5474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_584489, whole genome shotgun sequence.
ACCESSION   DASUTG010000903 DASUTG010000000
VERSION     DASUTG010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584489"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VF089_20965"
     CDS             complement(<1..497)
                     /locus_tag="VF089_20965"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX6176486.1"
                     /translation="MTLLPRPYHVPLARPFWNRATFRAILRCLLSGQVIVGPELAELK
                     SRITEKLGVQDAILCGSGSLALELALRSCDVKSGDEVMVPTFCCSNVIAPILAVDARP
                     VLVDIGDDLNITARTVEPALSKRTRAIIVPHLFGNPAEIDSIVDLTRRRDIRVIDDAA
                     QALGAT"
     gene            complement(692..1066)
                     /locus_tag="VF089_20970"
     CDS             complement(692..1066)
                     /locus_tag="VF089_20970"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176487.1"
                     /translation="MRVLKISELLEKPMDAATPVPGWTGGEVSRTRQAIIGEADSDNF
                     RCNVVNFRQGATTGWHTHNCDQILVVTAGRGSVANETEQREITVGDVVHIKAGERHWH
                     GATADTPMSHITVTTAGSQSKH"
     gene            1427..2401
                     /locus_tag="VF089_20975"
     CDS             1427..2401
                     /locus_tag="VF089_20975"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX6176488.1"
                     /translation="MKTSDLTHVARTFVQWFDSEHIDPADVNDGGDVDRINWLRTLPF
                     VILHFGCLAVFWVGWSSVAVQTAVVMYLVRMFAITGIYHRYFAHRTFRTSRWAQFAFA
                     VLGASSAQRGPLWWAAHHRNHHRESDLQADPHSPVQWGFLRSHAGWFMCNRYYATQYK
                     RIQDFARFPELVWLNRFDKAVPFALGFALYLTGAWLERSAPSLGVTGLQLFVWGFVVS
                     TTVLFHATASINSIAHLIGSRRYDTKDQSRNNLLLALITLGEGWHNNHHQHMGCTRQG
                     FYWWEIDLTYYVLKLMSWTGLIWDLKPVPARAYNRSEQLPIKKIASAA"
     gene            complement(2503..2859)
                     /locus_tag="VF089_20980"
     CDS             complement(2503..2859)
                     /locus_tag="VF089_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176489.1"
                     /translation="MKAGRLSAAGESRSCPHCKATILKSSVSCPLCRHVLKFGSAAAE
                     ASSNPTECPLSVEGTINHPGSGEALEYFILMEVRDESGKLVSRQSLGVGAIEKAQKRI
                     FTLRVEMSSTGMPPAP"
     gene            complement(2856..3359)
                     /locus_tag="VF089_20985"
     CDS             complement(2856..3359)
                     /locus_tag="VF089_20985"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6176490.1"
                     /translation="MALWNQPAQEDNGEKPHVEHAAPIAPVTPSHAPREQTSKDRRES
                     VFGPGVAIEGKIEGDADLRIAGKFKGDIQVKGDVNIERGAHLSAKISAVTVTIAGEVE
                     GNVVASAQVKLLESGQLIGDLKATTLTVAAGSRMRGHVEFGWSEAQAAKFVNGRAHEN
                     AKNGTNQ"
     gene            3947..4960
                     /locus_tag="VF089_20990"
     CDS             3947..4960
                     /locus_tag="VF089_20990"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX6176491.1"
                     /translation="MHRVPVEVPLLSEKITTSVLFARVETDEGITGYGLTRGTQRFGL
                     REFVNRELAPFLRDKNPLETERIWQQLYTAFNPRGQTGMWSSAVSAVDIALWDIKGKK
                     YNEPVWRLLGGAHNPVQSYVTFGLKQYSTEQLTEVAKQVVAQGEKRLKMVVAVDPENP
                     AVDAARVRAVRQAVGDDVELMIDANYLFSFNRALELCKLIEPYKITWFEEPVYQNDAL
                     LLADLRRHTSIPIAAGQNEGHRFRHRELIVNRGVDIAQPNVCSVGGYTEAVKVAALAQ
                     AFNLPIANGGGWPHHNMHLQAAMSNGWRVEFHFEMWGIGTRSTKSPQPRTKAGLRCRK
                     HPV"
BASE COUNT         1229 a         1490 c         1468 g         1287 t
ORIGIN      
        1 gttgcgccca gcgcctgggc tgcgtcgtcg atgactcgga tgtctcttct gcgtgtgaga
       61 tcgacaatcg aatcgatctc cgcaggatta ccaaaaagat gcggtacgat aatcgcgcgg
      121 gtccttttac ttaacgccgg ctcgaccgtt cgcgccgtta tattgaggtc gtctccgata
      181 tcgaccaaca cgggacgcgc gtccaccgct aagatcgggg caataacgtt ggagcagcaa
      241 aacgtcggga ccatcacttc atcccctgat ttcacgtcac aggatcgcaa cgcaagttcg
      301 agggcgaggc tacccgaacc gcacagaatc gcatcttgaa cacctagttt ttcagtgatg
      361 cgagatttca gctcggcaag ctccggccca acaataacct ggcccgagag caggcaacga
      421 agaatcgccc gaaaagtcgc gcgattccag aatggcctcg cgagaggcac gtgataaggc
      481 cggggaagca aagtcacgac tagacaatat cagactaaga tggctggcga tcactggaac
      541 atggggaatt ccggtcctgt cggcgcggca gctacgctga gccggcagtt ttgctgcgat
      601 acaaccaacg gtccggtccg ataatgcgca cggtcgcaaa gagcgggatt gtattcaacg
      661 aatgactgcg atcagttcgg tccaggccta gttaatgttt cgattgactg ccggctgtgg
      721 tgactgtgat atgtgacatc ggcgtatctg ccgtggcgcc atgccaatgc cgttcaccgg
      781 ccttgatgtg cacgacatcg ccaaccgtga tctcacgctg ctctgtttcg tttgctaccg
      841 aaccgcgtcc cgcagttacc accagaatct gatcacagtt atgagtgtgc caaccggtgg
      901 tagcgccctg gcggaagttc acgacgttac atcgaaaatt gtcagaatcc gcttcaccaa
      961 taatcgcttg acgggtgcgg ctcacctctc ctccagtcca tccgggaaca ggtgtcgctg
     1021 cgtccatggg cttttccaat aattctgata tttttagtac acgcatttac gcctccatag
     1081 agtcgattta cttcgcgtaa gacgaaatca caactacacc gccgtgaaca gacccgtcaa
     1141 gaaatcaatc gtgattatct agctgatttc ctaatcggca ctatctgaga tgaactcgct
     1201 ttatttcccg cgccgctcgc aaagcgcatt cttccggcgc gcacttgttt tccaactgct
     1261 cagaagcttg gctggtagta agtggatgct aatctacgga attgcctact gtgacatcac
     1321 gtcacttcga gcctagtccg cactctgcga attttccaca acaatgcgtc agtttcttac
     1381 caaaacattg aagcttccgc cgccccatgg taatagtgaa ggattaatga aaacgtcaga
     1441 cttaactcat gtagctcgca cgttcgtcca gtggtttgat tctgagcata tcgacccagc
     1501 tgacgttaac gatggtgggg atgttgaccg gatcaattgg ttgcgaacct tgccgttcgt
     1561 gatcttacac ttcggttgct tggcggtgtt ctgggtcggc tggagttccg ttgcggtgca
     1621 gaccgcggtg gtcatgtacc tggttcgaat gttcgccata acgggcattt accatcgcta
     1681 ctttgcacac cgaacttttc gcacttcccg ctgggcacag ttcgcatttg ctgttcttgg
     1741 tgcctcgtcg gcccaacgcg gtcctctttg gtgggcagcg catcacagga accatcatcg
     1801 cgagtcagac ttgcaagccg atccgcattc gccggttcag tggggctttc tccgctctca
     1861 cgccggctgg ttcatgtgca accggtacta tgcgactcaa tacaaacgga tccaggactt
     1921 tgcccggttt cctgaactgg tctggttgaa tcgtttcgac aaagccgttc catttgcgtt
     1981 gggattcgct ctctacttga ccggcgcttg gctggagcga tcagcgccct ctctcggtgt
     2041 gactggcctt caattattcg tttggggatt cgttgttagc accacggtat tatttcacgc
     2101 cacggccagt atcaattcga ttgcgcatct catcggcagc cgtcgatatg ataccaaaga
     2161 tcagagccga aataacttac tactagcgtt gattacactt ggagaaggtt ggcacaacaa
     2221 ccatcatcag catatgggtt gtacccgaca gggtttctac tggtgggaaa tcgatcttac
     2281 gtactatgtt ttaaagctta tgtcatggac tggactaatc tgggacttaa aaccggtacc
     2341 agctagagct tacaatcggt cagaacagct accgataaag aaaatcgcat cggccgcctg
     2401 agccgccgtt tgccccgacc cgccctgaac acccactcaa gagttaaacg aacttcacat
     2461 tgccgcgatc gagtcgtccg agcatcgcga ccgatctcgt cttcacgggg ccgggggcat
     2521 accggtcgaa gacatctcca ccctcaaggt aaagatgcgt ttttgcgcct tctcaatcgc
     2581 tccgacaccg agactctgcc gggaaaccaa ttttccggat tcatctcgta cttccatcaa
     2641 gataaagtat tcaagcgctt cgcctgagcc cgggtgattg atggtacctt cgactgatag
     2701 cgggcactcc gtaggattgg aagacgcttc ggcagcggcg gagccgaact tgagcacgtg
     2761 ccggcaaagc gggcacgaca ccgaactctt gaggatcgtc gctttgcaat gcgggcagct
     2821 gcgggactct cccgcagctg agagacgtcc cgctttcatt gattggttcc attttttgcg
     2881 ttctcatgag ctcggccgtt cacaaacttt gccgcttgtg cttcgctcca gccgaattct
     2941 acatggccgc gcatccgcga accggccgcc accgtcaacg tcgtcgcttt gagatcgccg
     3001 attagctgtc cggattccag cagcttcacc tgcgcgctag cgacaacgtt gccttctacc
     3061 tcgccggcta tggtaacggt taccgcgctg atcttcgccg acagatgcgc acccctttcg
     3121 atattcacgt cgcctttgac ttgaatatcg cctttgaact tgccggcaat acgcaggtcg
     3181 gcatcacctt cgatctttcc ctcgatcgca acgcccggtc caaaaacgga ctcacgacga
     3241 tccttggatg tctgttcacg cggcgcatgg ctcggggtta ccggagcgat cggcgcggca
     3301 tgctcgacgt gcggcttctc accgttatct tcttgtgcgg gttgattcca aagtgccata
     3361 ggacctcctt aatgtttgat gacagcggac gacaattatg gactgaagct tggagccgga
     3421 acttctccaa cccgacgcaa tggaggtgcc agctgtcaat tgcaggacag ttgcacgtcc
     3481 cgggaggtca gaactatcgc gaactcggct caccgaggca cccacaaaga cttttgggag
     3541 cgtgaggtcg cgttagttag aaagaattca gcaaaccccg cttaagctgt tggaaaaagc
     3601 gagccgcgcg gaaggttttc agatgctcca caaatgtcgc cggaaagcta gatttgtaca
     3661 caggggcgcg ccgccatcgc gcggcgttgg tattgagatt ttctcattcg gcaagattga
     3721 agaaatcgaa tattgaagtg ttgtggaagc ggagatcatg tcgagcgtgc gacatgttta
     3781 tcaatctgct gatggcgatt cacatttgac aagggatgaa cagagctgta ccgctagccg
     3841 tagccaaagc ggcaattcta tcgaagacaa ttcacggtga atttatctaa cccaatccct
     3901 atccgaaacg aggtgcggtc tgaaaatcac cgaggttaaa gcaacattgc atcgagttcc
     3961 tgtcgaagtg ccgttgctgt cggagaaaat cactacttcg gtgcttttcg ccagggtcga
     4021 aaccgacgag ggtataaccg gttacggtct tacgcgtggc acgcaacgat ttggcctgcg
     4081 agaatttgtt aatcgtgaac tcgcgccgtt cctgcgcgat aagaatccat tggaaaccga
     4141 acggatctgg cagcagctct acaccgcctt taatccacgc ggccaaaccg gcatgtggag
     4201 ctcagcggta agcgccgtcg acattgcgct atgggacatc aaagggaaga agtataacga
     4261 gccggtctgg cgcctgcttg gcggcgcgca caatccagtc cagtcgtatg taacttttgg
     4321 gctcaagcaa tacagcacag aacagttgac tgaggtcgcc aaacaggtag ttgcccaagg
     4381 ggagaagcga ctaaaaatgg tcgttgccgt ggatccggaa aatcccgccg tcgatgcggc
     4441 gcgcgtgcgc gctgtgcgtc aggcggtggg agatgatgtg gaattgatga tcgatgccaa
     4501 ttacctcttc tcattcaacc gcgcgttgga gctttgcaag ctgatcgagc cttataagat
     4561 cacctggttt gaagaaccgg tttatcagaa tgacgccctg ctgcttgccg acttgcgccg
     4621 acatacgtcg attccaattg ctgcggggca aaacgaaggg catcgatttc gtcatcgaga
     4681 actgattgtc aatcgtggtg ttgacatcgc gcagccaaac gtttgttcgg tgggcggtta
     4741 tactgaagcc gtcaaggttg ctgcgctggc acaagcgttc aatttaccga tcgctaacgg
     4801 cggtggttgg ccgcaccaca acatgcatct gcaagcggcg atgtcgaatg gctggcgggt
     4861 cgagtttcac ttcgaaatgt ggggcatcgg gacaagatct acaaagagcc cgcagccccg
     4921 gaccaaggct gggttacgct gccggaaaca cccggtttag gattggagcc gcgctgggac
     4981 gcgcttaagg agttcgaaga gaaataatgg gcaaaacctg caagttttct ctgagccgga
     5041 ttccggcagg ttttgcagcc ccatagggat gaaaatcgct gaagtgcctc ttaaacctgg
     5101 cggcgaatgc cggcagccgt ccgactggaa gcttacacat agtccatagt cgcacttcat
     5161 ttaacctggt gtttgaacat catgcatgca acgttcgttc tttgtgcgcg aagcaaggcg
     5221 gcgggcgcgc atggaaaatt caacacggct tattcatttg acatgacgct ggtagctcgg
     5281 ctactgtcgc gccgattcag tcccgaattc cgccggctct ctttagttat gagacagcaa
     5341 taaggagaca aatgcctgcg cgcagcggcg ccgaatatct ggcggggctc gccgatcaac
     5401 gcgagatctg gtttgacggc gatcgcgtcg ggagcgtcgt cggccatccg attcttggcc
     5461 gcatggcgcg tacc
//
LOCUS       DASUTG010000904         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_436753, whole genome shotgun sequence.
ACCESSION   DASUTG010000904 DASUTG010000000
VERSION     DASUTG010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436753"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..431)
                     /locus_tag="VF089_20995"
     CDS             complement(51..431)
                     /locus_tag="VF089_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176492.1"
                     /translation="MAAHLPEAKESLWLLTASPVIWAAHFLLCYTTAAVWCAKVVGLG
                     GPLDGARIAIGVYTLLALAAIGVIGWIGHHRHSFGQANLPHDDDTPEDRHRFLGFATL
                     LLSALSGVAVVYAAIVAVFVRSCY"
     gene            complement(599..>2297)
                     /locus_tag="VF089_21000"
     CDS             complement(599..>2297)
                     /locus_tag="VF089_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX6176493.1"
                     /translation="VALIAMIVPTFAQRAIVGYSWIVLAAVGTGFLSFGLWVHHMFTT
                     GLPGISLGLFSAASQAVALPTGVQIFCFIATLAAGRVVNSVPMLYAFGSLVIFVLGGL
                     TGVMVALVPFDFQAHDTFFVVAHFHYVLIGGALFPIIAGCYYFFPLINGKMLSDKLGK
                     IAFWLAFTGFNIAFLPMHLTGLRGMPRRVFTYPADIGFDVLNLTSSIGAFILAAGIII
                     VFWDLVRPKKNQPYAERNPWRAGTLEWTQEMPGKPWGIRSIPEIDSRYPLWDQPNFMR
                     DVDEGRFYLPDAEEGERETIITSVIDAQPIQCARLPGPTFIAFWAALTTGGFFIFGTY
                     HWWPPAIMSLVLALGVIIYWLWTGTAIIPEKAEKDVGLGLTLPLYASGSDSVGWWAMF
                     ITMLAMLSAFMSLIFGYFFFWTVRTDFPPDPSSGPGLFWPLTAAALLLAAWALTVLAK
                     RWNTNDSAAGFYGGLMMAVVLAIAGGASLLAGPWMTGLNPVSHVYPATVWILVIWSAL
                     QITIGVIMQLYCIARRIARRLTARYDADIGNVTLYWHFTALTVTVTVAVIAGFPLVV"
BASE COUNT          563 a          682 c          670 g          382 t
ORIGIN      
        1 ccagaccgca gcgaggatca gcaatcccac gactatgaca ccgcggcgca tcagtaacag
       61 ctccggacga agaccgcgac gatggccgca taaacgacag cgactccgct cagcgccgat
      121 agcagcaggg tggcaaagcc caggaatcgg tgcctgtcct caggcgtgtc atcatcgtgc
      181 ggcagattag cctggccgaa gctatgtcgg tgatgtccaa tccatccgat gaccccgatg
      241 gcggcgagcg ccagcaacgt atagacgcca atcgcaatcc gagcgccgtc cagcggaccg
      301 ccaaggccga ccactttcgc gcaccacact gcggccgtgg tgtagcacag caggaaatgc
      361 gccgcccaaa ttacgggcga ggcggtgagc agccacaggc tttcctttgc ttcaggcaaa
      421 tgagctgcca tgtattacct attgaccggg gaattgttcc aatcgatcaa gccgttcaat
      481 cgctccgctc cgttcagacc atcctctttc ctccgccgcg acgcggggaa gatgaaggtg
      541 ggggaggcaa cgatttgaac ggcttcaact gttggaactg tgttgaacgc attcatcttt
      601 atacaaccag cggaaaaccg gcgatcacag ccacggtaac ggttacggtc aaagcagtga
      661 agtgccagta cagcgttacg ttgccgatgt cggcgtcgta acgggctgta agtcgtcggg
      721 cgatccggcg cgctatgcaa tacagttgca tgatgacacc aatggtgatc tgaagcgccg
      781 accagatcac cagtatccaa acggtggccg gataaacgtg actcactgga ttgagcccgg
      841 tcatccatgg accggcgagt agcgacgcgc ccccggctat ggccaaaacg acagccatca
      901 tcaatccgcc atagaaacca gcggcgctgt cgtttgtgtt ccaacgcttt gccagcaccg
      961 tgagcgccca tgcagcgagc agtagcgccg cggctgtcaa cggccagaag agccccggac
     1021 ccgaggacgg atccggcgga aagtcggtgc gcacggtcca gaagaagaaa tagccgaaga
     1081 taagcgacat aaacgcgctg agcattgcca gcatggtgat gaacatcgcc caccaaccga
     1141 cggaatcgga acccgaggca tagagcggca atgttaaacc caggccgaca tccttctcag
     1201 ctttttctgg aatgatcgcc gttcccgtcc acaaccagta aatgatcacg ccgagcgcca
     1261 gcacgaggct catgattgcc ggcggccacc aatgataggt gccgaagatg aaaaacccgc
     1321 cggtcgtgag cgcggcccag aacgcaatga acgtcggccc gggcagccgc gcgcattgta
     1381 tcggctgcgc gtcgatgacc gaggtgataa tggtctcgcg ctctccttct tcggcatccg
     1441 gcagatagaa tcgcccctcg tcgacgtcgc gcatgaaatt gggctgatcc cagagcgggt
     1501 agcggctgtc gatttcgggg atcgaacgaa ttccccaggg cttcccgggc atctcttgcg
     1561 tccattccag ggttccggcc cgccacggat tgcgctccgc ataaggctgg ttttttttcg
     1621 gtcggaccag atcccagaag acgatgatta tgccggcggc cagtataaaa gcgccgatgc
     1681 tcgaagtgag gttgagcaca tcgaagccga tgtcggcggg ataggtaaaa acgcgccgcg
     1741 gcataccgcg cagcccggtc aaatgcatcg gcagaaaggc gatgttgaag ccggtgaacg
     1801 caagccagaa ggcgatcttg ccgagcttgt ccgaaagcat tttgccgttg atgagcggga
     1861 aaaaatagta acaaccggca ataatcggaa aaagcgctcc gccgatgaga acgtaatgaa
     1921 aatgagcgac gacaaaaaag gtgtcgtgcg cctgaaagtc aaacggcacc agcgccacca
     1981 tgactcccgt caacccgccc agcacgaaaa tcaccaggct gccgaatgca tagagcatgg
     2041 gcaccgaatt aaccacgcgt ccggcggcca gtgtcgcgat gaagcagaag atctgcacac
     2101 cggtcggcag tgctacggcc tgcgaggcgg ccgagaacag gccgagtgag ataccgggaa
     2161 ggccggtggt aaacatgtgg tgcacccaca gcccgaaact caagaagccg gtgcccaccg
     2221 ccgcaagcac gatccagctg taaccgacga tggctctttg cgcgaaagtc ggcacgatca
     2281 tcgcgatcag cgccacc
//
LOCUS       DASUTG010000905         8007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2068024, whole genome shotgun sequence.
ACCESSION   DASUTG010000905 DASUTG010000000
VERSION     DASUTG010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068024"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF089_21005"
     CDS             complement(<1..231)
                     /locus_tag="VF089_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6176494.1"
                     /translation="MKETPKRIVIVGGGFGGLYAAKTLSNEAVEVTLIDRKNHHTFQP
                     LLYQVALAVLSPAEIASPLRHILHKSRNVLTIL"
     gene            414..1988
                     /locus_tag="VF089_21010"
     CDS             414..1988
                     /locus_tag="VF089_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX6176495.1"
                     /translation="MKMTLKHCPHKGLGLYIMFLSLSVGLWGGCSTLPSLVNRTASTA
                     IINTGNTKLGQRISPLVESHPGKSGIYPLPDARDAFAARALLAQAAERTLDLQYYIWH
                     GDMSGTLLLNAVRDAADRGVRVRLLLDDHGTSGIDTTLAALDSHPSIEVRLFNPFVIR
                     NPRGINYLFDFFRLNRRMHNKSFTADNQVTIIGGRNVGDEYFGATDGLLYVDLDVMAV
                     GPVVSEMSKDFDRYWASKSSYPVAGLLPAVSPARIAEIASAASLIKEDPAAIAYIDAI
                     RNSSFIRELVERRLPMQWAATRMISDDPAKGLGLAPPEGLLLQKLKEIIGEPQTKVDL
                     VAPYLVPGAEGTDAFVELAKRGVKLRILSNSWEATDAVAPVHAFYTKWRTSLLEGGIE
                     LYELRRLSPAVEARKNDGPSGSSSSSLHAKTFSVDRARVFIGSFHFDPRSAELNTELG
                     FVIESPALAQAIENAFDNRISASAYEVRLSDDGQIYWIEQRDGEVVRYDTEPETTFWQ
                     RAGLRLMSFLPIEWLL"
     gene            complement(2211..2828)
                     /locus_tag="VF089_21015"
     CDS             complement(2211..2828)
                     /locus_tag="VF089_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6176496.1"
                     /translation="MKTHPFLIGITILLSTLMLSLLPYKPAMAHCDSMDGPVVKDAQR
                     ALTEKKVTPVLKWVRGEDEEPVRKAFDMTLAVRGESERAKSLADRYFFETLIRIHRAS
                     EGEGFTGLKPAGSVDPAIAAADRALADGNIDPLADKLATGVRHEIKERFTEARTKRQV
                     AEQSVEQGREYVKAYVQLTHFAEGVEHLTSHGASPKHRENKEEGH"
     gene            complement(3131..4252)
                     /locus_tag="VF089_21020"
     CDS             complement(3131..4252)
                     /locus_tag="VF089_21020"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX6176497.1"
                     /translation="MNQEKPTKILQVLAILLLCFPFPFALHAASGDDFIRGYASAVLQ
                     REFRLKNFSLNVTGEAVTVRSGELTEADHGKVIAALSSIDGVKRVAILDSRGVEVATS
                     SAQASETQRAIRQMPVPEYELGFLPGGNLFEPPVADPRWPRFSIGYRYFIDEAANVAS
                     ATFGETIALYRHRGPYDGLWEIGFQAGVFSIFDLDSSSFDLVNSDFFAGLHATYQIGD
                     LSAFFRVFHQSSHLGDEFLLRNRIDRINLSFEGLDLKLSYPFFDWLRLYGGSAYLFHR
                     DPSDLKPWSTQGGIELQTPWRFWADSARFVTALDLQNREENKWSTEISVRGGLQFERA
                     QRFGRKISLLVEYYKGHSPNGQFFNRKVEYFGPGLQLDF"
     gene            complement(4416..4745)
                     /locus_tag="VF089_21025"
     CDS             complement(4416..4745)
                     /locus_tag="VF089_21025"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6176498.1"
                     /translation="MKMIMAYIRTECSADVMRDLYNAGVGESPAMWCTEDAVNGQLFS
                     IRPAHSKIHHLPASLKLGVVCPDERCDEIVRLIGRTARTGNRGDGIITVLDVDNVQRI
                     RELEASS"
     gene            complement(5032..7470)
                     /locus_tag="VF089_21030"
     CDS             complement(5032..7470)
                     /locus_tag="VF089_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX6176499.1"
                     /translation="MAETLSKEQLRLLDAYWRAANYLSVGQIYLYDNPMLKEALSKEH
                     IKPRLLGHWGTTPGLNFIYAHLNRVINARDLDMIYITGPGHGGPALVANAYLEGTYSE
                     VYPHISPDAEGMKKLFKQFSFPGGIPSHVAPETPGSINEGGELGYSLSHAYGAAFDNP
                     DLVVACVVGDGEAETGALATSWHSNKFLNPITDGAVLPILHLNGYKIASPTVLARISH
                     EELDQLFRGYGYTPYFVEGHEPEKMHQRMAATLDRVIDQIKQIQSDARSGKARGDRPR
                     WPMIVLRSPKGWTCPAEIDGKRTEDYWRSHQVPMGEMHSNPAHVKLLEEWMKSYKPKE
                     LFDENGRLKSEIAALAPQGERRMSANPHTNGGILLRGLRLPDFRSYEVEVSEPGAETA
                     EATRVQGRYLRDVMKLNLESKNFRLFSPDENNSNRWQDVLEVTNRAWNAERFDYDDKL
                     APNGRVMEMLSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLKVCNDI
                     PWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVINKKAEVVRVYLPPDANCLLSV
                     TDHCLRSRNYVNVIIAGKQPAPQWLNMDQAVKHCTAGIGIWEWASNDRGDEPDVVMAC
                     CGDVPTLETLAAVELMQRHLPELKMRVINVVNLMTLQSPHEHPHGLSDKDFDALFTKD
                     KPIIFAFHGYPWLIHRLTYRRTNHNNLHVRGYKEEGTTTTPFDMCVLNDLDRYHLVQD
                     VIDRLPQLGARAAYAKQAIRDALIDHKEYIYEHGEDAPEIRGWTWGNKQIAGHLDSTE
                     GDNIDAGVEAPIRN"
     gene            7765..>8007
                     /locus_tag="VF089_21035"
     CDS             7765..>8007
                     /locus_tag="VF089_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176500.1"
                     /translation="MVTGILSLLITLLLASSALANPYLPKAGEKPLSMRVSTCAVSGG
                     FVHLYTALDNGLFDKYGIKLENIFLRGSAQNLTALAT"
BASE COUNT         1755 a         2229 c         2133 g         1890 t
ORIGIN      
        1 cagaatcgtt agcacgttgc gcgatttgtg cagtatatgc cgcaacggcg atgcgatctc
       61 ggccggcgag agcactgcca gcgccacttg atagagcaac ggttgaaacg tgtggtgatt
      121 cttgcgatcg attagagtta cttccaccgc ttcattcgac aatgttttcg ctgcataaag
      181 accgccgaag ccgccgccga cgatgactat tcgtttaggt gtttctttca ttgaagcttt
      241 tccgtatcga tcgtttagtt tgattgaccc agctatttga ccgccttaat gtggagtata
      301 aattttccat tcgcgaaaga gtctagattc aagcgggaag cagaaacagt ttttaggcta
      361 taactataag ccgctcaatc aagtacgcag ctgtacgaat ctccaccagg tctatgaaaa
      421 tgacactcaa gcattgcccg cataaaggcc ttgggcttta catcatgttt cttagtctga
      481 gcgtggggct atggggcgga tgcagcactc tgccctcgct cgttaaccgc acggcttcta
      541 cggcgattat caacaccggc aataccaaac ttggacagag aatttcgccg ctagtcgaat
      601 ctcatcctgg aaagtccggc atttatccgc tgccggatgc gcgcgatgct tttgccgcgc
      661 gtgcgctatt ggcccaggcg gcggaacgga ctctcgatct tcagtattac atttggcacg
      721 gcgacatgtc gggaaccctg ctattgaatg cggttcgcga cgccgccgat cgcggcgtcc
      781 gagttcggct gttgctcgac gatcacggca cctccggaat agacacgact ctcgccgcgc
      841 tcgattctca ccccagtatc gaagttcggc tgttcaatcc ttttgtgatc cgtaaccccc
      901 gcgggattaa ttacctcttc gacttcttcc ggttaaaccg gcgcatgcac aacaaatcgt
      961 ttaccgcgga caatcaagtc acgatcatcg gcggccgcaa cgtgggagat gaatacttcg
     1021 gcgctaccga cggactactg tacgtcgatc tcgacgtcat ggcagtcggt cccgtggtca
     1081 gtgagatgtc aaaggacttt gaccgttact gggccagtaa atcgtcatat ccggtagctg
     1141 gattattacc ggccgtcagt cccgctcgta ttgcagagat cgcatcggcg gcgtcgctga
     1201 ttaaagaaga tcccgcagcc atcgcttaca tcgacgccat acgcaactct tcatttattc
     1261 gtgagctggt ggagcggagg ttacccatgc aatgggccgc cacccgcatg atcagcgatg
     1321 accctgccaa ggggctcggt ctggctccgc ctgaagggct gttactgcag aagttaaaag
     1381 aaatcatcgg cgagccgcaa accaaagtgg atcttgtggc gccttatctt gtacccggcg
     1441 cagagggtac tgatgcgttt gtcgagttgg ccaaacgcgg cgtcaaactc aggatactga
     1501 gcaactcgtg ggaggccacg gatgcggtag ccccggttca tgccttctac acaaagtggc
     1561 gcacgtccct cttggagggc ggtatcgagc tctacgagtt gcgccgtttg tcgccggccg
     1621 tcgaagcaag aaaaaacgat gggccaagcg gaagttcgag ttcaagcctg cacgcgaaaa
     1681 cgttttcagt ggatcgtgcg cgcgttttca tcggctcgtt ccatttcgat ccgcgttcgg
     1741 ccgagctgaa caccgagttg gggtttgtga tcgaaagccc ggcattggcg caggcaatcg
     1801 agaacgcttt cgataacaga atttccgcca gtgcttatga agtacggctg tcggatgacg
     1861 ggcagattta ttggatcgag caacgcgacg gagaagtggt gcgctatgac acggagcctg
     1921 aaacgacttt ttggcaacgc gcagggctgc ggctcatgtc gttcttgcct atcgagtggc
     1981 tgttgtgatg agagacgcca gtccgacgtt aatcaccaag cctgtgtctg gaggtttgtt
     2041 cgttccaatc ccagcaagag agctatccaa tctttcaggt cagggagggt acgtgaagaa
     2101 gccatgaaag cccctcgtat ctagcctgca ctggcgccgg aattgggatt ccgtcacccg
     2161 ggttatcacg accatagcta cctaacacca tagcttgcct accgcctcgg tcaatggcct
     2221 tcttccttat tttcccggtg cttgggactc gcgccgtgcg acgtaagatg ctccacgcct
     2281 tcggcaaaat gagtgagctg gacgtaggcc tttacatatt cccgcccctg ctcgacagac
     2341 tgttcggcca cctgccgctt cgtccgggct tcagtgaagc gctcttttat ttcatgccgc
     2401 acccccgttg ccaatttgtc ggcgagcgga tcgatgttgc cgtcggccag agcgcggtca
     2461 gcggcggcga tcgcgggatc cacgctcccg gccggtttca gccccgtgaa gccttcccct
     2521 tcgctggcgc ggtgaattct gatcagagtc tcgaaaaagt agcgatcggc gagactcttc
     2581 gcacgttcgc tttcgccgcg aaccgccagt gtcatatcaa aggccttgcg gactggctct
     2641 tcatcctctc cacgaaccca tttcagaact ggggtcacct tcttttcggt gagcgctctt
     2701 tgagcgtctt tgacgaccgg gccgtccatg ctgtcgcagt gggccatcgc cggtttgtat
     2761 ggcaatagag atagcatcaa cgttgagagc aggatggtga tgccaatgag aaatggatgg
     2821 gttttcatgt gaacctccct atactgatcg aatcacctga ctgcagcgta atcactctgg
     2881 tgcaacccct cgcgccgata gtttcgtcgc acaaggacga aatgcagcaa tgggttagaa
     2941 ctgagtatga catcgtgacg agctgaattt ctcgtgcaag acttgcaatg atggattctt
     3001 attagcgcaa catgtcgatg ccggcttgtt ccgtaaaaga tttccacagg cttggaatct
     3061 cgccagtcga gcgggcacac gcgccttgac ggatgggctt tcttgccgct cgcgcgaact
     3121 cgcgaggtca ctaaaaatcc agctgcagcc ccggtccgaa atattcaacc tttctattaa
     3181 aaaactgtcc gttgggagaa tgtcccttgt aatattcaac cagtaaactg atcttccggc
     3241 caaacctctg tgcccgttca aattgaaggc cgccacggac cgagatctcc gtgctccact
     3301 tgttctcttc ccggttttgc aggtccaaag ccgtaacgaa tcgcgctgag tccgcccaaa
     3361 atctccaagg cgtttgcagc tcgataccgc cctgtgttga ccagggttta agatccgagg
     3421 gatctcggtg aaatagatag gcgctgccac cgtaaaggcg aagccaatcg aaaaatgggt
     3481 aagacaactt caggtccagg ccttcgaagc ttaggttgat gcgatcgatt cggttgcgca
     3541 gaaggaactc gtcgcctaga tgcgagctct gatggaagac gcgaaaaaac gccgacagat
     3601 ccccgatctg gtaggtcgcg tgcagcccgg caaaaaagtc actgttgacc agatcaaacg
     3661 atgacgaatc cagatcgaag atcgaaaata cacccgcctg aaagccgatc tcccacagtc
     3721 cgtcgtaagg gccgcgatga cggtaaaggg caatggtttc tccaaaggtc gcggaggcga
     3781 catttgctgc ttcatcgata aaataccggt agccgatcga gaagcgcggc cagcgaggat
     3841 cggcgaccgg cggctcaaaa agattgccgc ctggaagaaa gccgagctcg tattcgggta
     3901 cgggcatttg tcggatagcg cgctgtgttt ccgatgcttg cgcgctcgat gtagccactt
     3961 caactccgcg ggaatcgagg atggcgaccc gcttcactcc atcaatcgat gatagcgcag
     4021 caatgacctt gccgtgatcc gcttcggtca gctctccgga tctcaccgtg accgcttcgc
     4081 cggtaacgtt cagggagaaa ttcttcagac gaaactctct ctgcaatacg gcggacgcat
     4141 agccacgaat gaagtcatcg ccgctagcgg cgtgtaatgc aaaaggaaaa ggaaaacaca
     4201 ggagcaggat cgccaggacc tgcaaaattt ttgttggctt ttcctgattc attgcgttct
     4261 cctcactacc ggggcgattt ttcccctgct ctccctttaa ctgggtctga tcattgtgtc
     4321 acaacttacg atcaagcgcg ttctttccga atatttagtg aattcgcaag actcaatcac
     4381 gtcctgctag gcaacgaagg tccctgcgaa ggacatcacg atgatgcttc gagctcgcga
     4441 attcgctgca cattatccac gtcgagcacg gtgataatgc cgtcgccgcg atttcccgtg
     4501 cgcgccgtgc gcccgattaa cctgacgatc tcgtcacagc gctcgtctgg acaaaccact
     4561 ccaagcttca aagatgcggg gagatgatga attttcgaat gggcgggtcg aatagagaaa
     4621 agttggccgt tcactgcgtc ttccgtgcac cacatagcag gtgattcccc gacgccggcg
     4681 ttgtacaaat cgcgcatgac gtcggcggag cattcggtgc gtatgtaagc catgatcatt
     4741 ttcatgattc acccctctcc tttgaaaaaa cttgcgccaa tccgccagga gcgaaaactt
     4801 cgaaaaacat tgccggggct acggaggctc ctctacaaag ttggaacgac aggcaacgga
     4861 tgaagctgcg atcgaaaatc cgaaattcga ataccgaaat ccgaaacgat ctcaacattc
     4921 tgaaagttca aatccaaaac tcaacggcga ctgaatctcg tttgaaacat tcggtcttgg
     4981 tcattttgat attgttttaa gcttcgagct tcggatttcg tgcttagaat cctagtttcg
     5041 aatcggcgct tcgactcctg cgtcgatgtt gtcgccttcc gtggaatcca agtggccggc
     5101 tatctgtttg tttccccagg tccagccgcg gatctcgggc gcgtcctcgc cgtgctcgta
     5161 gatgtattcc ttgtgatcga tgagcgcgtc gcggatcgct tgcttggcgt aggccgcccg
     5221 cgcgcccaac tgcggcagcc gatcgatcac gtcttgcacc agatgataac ggtccaaatc
     5281 gttgagcacg cacatgtcga acggcgtggt cgtggtgcct tcttccttgt agccgcgcac
     5341 gtgcaaattg ttgtgattgg tgcgccggta ggtcaggcga tggatcagcc agggatagcc
     5401 gtgaaaagca aagatgatcg gtttgtcctt ggtgaacagc gcgtcaaaat ctttgtcgct
     5461 caaaccgtgc gggtgctcgt ggggcgattg caaggtcatc aggttgacta cgttaatgac
     5521 acgcattttt agctccggca gatgccgctg catcagctcc accgctgcga gagtttccag
     5581 agtcggcaca tcgccgcagc aggccatcac cacgtccggc tcatcgccgc ggtcgttgct
     5641 cgcccattcc catatgccga tacccgcggt gcagtgcttg actgcttggt ccatattgag
     5701 ccactgcggc gccggctgtt ttccggcaat gatgacattc acatagttcc ggctacggag
     5761 acagtgatcc gtcactgaaa gcaggcagtt cgcgtcgggc gggagataga cccgcacgac
     5821 ctcggctttc ttattaatca cgtgatcgat gaacccgggg tcctgatggc tgaaaccgtt
     5881 gtggtcctgg cgccacacat gagaggacag caggtaattg agcgatgcga tgggcctgcg
     5941 ccacgggatg tcgttacata ccttcaacca tttagcgtgc tggttgaaca tcgagtcgat
     6001 aatatgaatg aaagcctcgt aacacgagaa gaaaccatgc cgtcccgtca ggaggtagcc
     6061 ttcaagccaa ccctggcact ggtgctcgct cagcatttcc atgacgcggc cgttgggcgc
     6121 aagcttgtca tcgtaatcga aacgctccgc gttccaggcg cggttagtca cctcgagcac
     6181 gtcctgccag cggttcgaat tattttcgtc cggactgaac agtcggaaat tcttgctctc
     6241 aagattgagc ttcatcacgt cgcgcaggta tcggccctga acccgggtgg cttcagctgt
     6301 ttccgcccct ggttctgata cttctacttc gtaacttcga aagtccggaa gtcgcagccc
     6361 tctaagcaat atgccgccgt tggtgtgcgg gttggcgctc atgcgtcgct cgccttgtgg
     6421 cgcgagcgcg gcaatctcgc tcttcaatcg tccattctca tcgaacaact ctttcggctt
     6481 gtaactcttc atccattctt cgaggagctt tacgtgagcg ggattgctgt gcatctcgcc
     6541 catgggcacc tgatgcgagc gccagtaatc ttcagtgcgc ttgccgtcga tttccgcggg
     6601 gcaagtccat cctttcgggc tacgcaacac gatcatgggc cagcgcggcc ggtcccctcg
     6661 cgccttgccg ctcctcgcat cactctggat ctgtttaatc tgatcaatga cccggtccag
     6721 ggtagcggcc atccgttgat gcatcttttc cggttcatgt ccctcaacga agtacggtgt
     6781 atagccatag ccgcgaaaca gctgatccag ctcttcatga ctgatccgcg ccaataccgt
     6841 gggactggcg atcttatagc cgtttaggtg caagatgggc agcaccgcgc catcagtaat
     6901 tggattcaag aatttgttcg aatgccaact cgtcgccaac gcgccggttt ccgcttctcc
     6961 atcaccgacc acgcaagcga cgaccagatc aggattgtcg aaagcagcgc cataagcgtg
     7021 cgaaagggaa tagcccaatt cgccaccctc attgatcgat ccgggagttt ccggcgcgac
     7081 gtgactgggt atgccgcctg gaaacgagaa ctgcttgaac agtttcttca tcccttcggc
     7141 atcgggtgag atgtgaggat agacttcgct gtaggttccc tccagataag cgttggcaac
     7201 caaagcgggc ccgccatgcc ccgggcccgt gatgtaaatc atgtccagat cgcgcgcgtt
     7261 gatgacccga ttgagatgag cgtagataaa gttcagcccc ggcgtggttc cccaatgacc
     7321 caacagacgc ggcttgatat gctccttgct gagcgcttct ttcaacatcg gattgtcgta
     7381 aagataaatc tgtcccaccg ataaataatt cgccgcgcgc cagtaggcat ccagcagcct
     7441 taactgctcc ttggatagtg tttccgccat agtgttctcc tttctaatta acttagccga
     7501 caaagccgtc agcgcaatga gtttccttcg aattttttca aaaaactgcg ggattagtag
     7561 cggagaggat cttcgtctct cccgcagcaa gaaccccgtt catcgcgaaa gattcatggc
     7621 gccatgagat tttttcggat gtacggaaag ccggataggg cttcaaaccg catgaagttg
     7681 ccgcgcaatc ggcgggaaat gaaaatagcg cgctcgcggt ttctgtttac aagcaaccgc
     7741 aatcaggagt agtatccagc gcaaatggtc acgggaattt tatcgttgct gattacgctt
     7801 ctgctcgcct ccagtgctct ggccaatcca tatcttccga aagccggcga gaagcctctg
     7861 tcaatgcgcg tctctacgtg tgcggtgtcc ggcggtttcg tgcatctcta cacggcgctc
     7921 gacaatggcc tgttcgacaa atacggcatc aagctcgaga acatattcct tcgcggcagc
     7981 gcccagaatc tgacggctct tgccaca
//
LOCUS       DASUTG010000906         4833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1771946, whole genome shotgun sequence.
ACCESSION   DASUTG010000906 DASUTG010000000
VERSION     DASUTG010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771946"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VF089_21040"
     CDS             <1..874
                     /locus_tag="VF089_21040"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX6176501.1"
                     /translation="IVSSLNDALVRRLVYLGEHSLGAPPAPFAWIVFGSEGRLEQALL
                     TDQDNALVYESESAAARSYFTALANYLVDALIQVGFPPCAGGYMATNWCKSLNQWQEL
                     FIRHIQTPEPQALLDSAIFFDFRAVAGDLSLAPLEKILAGARHEKLFLYHMIRGALEF
                     PPPLGFFNRVRTDNGKVDLKKGGIGPIVSLARAAALCAGSHERPTLARLEAAGASGSL
                     LSNENARTLSDIFSFLLHLRLRRQLTAYRHKEPLDHAISLAELSTLERRHLQEAFVMI
                     KRIQDALMLDSHYP"
     gene            935..1753
                     /locus_tag="VF089_21045"
     CDS             935..1753
                     /locus_tag="VF089_21045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176502.1"
                     /translation="MKLVPIDEAHPPSSNLWLARLREVVSNWLGIIGILIFFLAWEIA
                     TRTHILNPFYFPPFSQILAKGYELFANGSIWEHMRFSLTNFAIGFLISVMLGVVIGVP
                     MGWYRSLSKLFDPLLSGIYATPLIALLPLIIMIFGLGPVSKIIMTILAAVFPILINTM
                     TGIANTDHRLITMARAFGAKDSTIFLKVSVPGSLPYIVAGMRVALGRALVYIVVAEQY
                     GAATGLGYLSSVAAQRFQMSAMFVPIVIIAALGASLNELLKVLEQRLDKWKPRK"
     gene            1788..2783
                     /locus_tag="VF089_21050"
     CDS             1788..2783
                     /locus_tag="VF089_21050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176503.1"
                     /translation="MNSAKILLCVSVLTLTVVAAWSNSSRAQGAKMTVGQTGTNPGTS
                     LFFIAEKEKLFAKHGLDVNIIKSNTAAAVQAMLSGSMHMATGSGAAAFTTATIEGAPP
                     FVLVGSWVNVFPYKIMALKEIAKIEDLKGKTGHVGAPFGTIPDTALRFALAKLKLDPE
                     KDVKLVQNASADPAAILASMDRGDVQFGILAAPFDRVAERRGYRTLLALPELGIPWQQ
                     NGEWVQRSYLKSNRDNVVRFMKAISDAMNIYFGQKEKTVAYLTEFMGSKPEDTEYAYE
                     LFAKWADRSPRPKVETVRTTLEAIKKNTPKAASVDPASFIDTSIVDQLIKEGHVK"
     gene            2856..3992
                     /locus_tag="VF089_21055"
     CDS             2856..3992
                     /locus_tag="VF089_21055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6176504.1"
                     /translation="MKLYNFDLLAYPHVPKDAPRTPVPSNFFDPIKGAANYAEHLEEM
                     TYCEELGLDGVVFNEHHYSSYGTMPSPNLIAAALSQRTKRIKIGVLGNILPLRNHPVR
                     VAEEYAMIDCLSNGRLIAGFVRGIPAEYVWYGVNPEESRGRFQEAYDLIMTAWTNSVW
                     SFEGEFYKLKDCAIWPRPLQQPHPPIWIAARSAESIEWCVKRHLPCAQVYQTTAQIED
                     TFNYYRTKAKEDGWEARPDDFILCRHIYIDESNKKAQEVAEPAMRYFFSVYNRGFNDA
                     INQTAADQQKLLAKLTSDRSFNYFREGNRSRIDFSTLGWDELVKSGYMIAGDPDSVAR
                     QIQDQMKQIGADHFMGMFHIGNLAHEKVISSLDLFKKEIMPRLN"
     gene            4145..>4833
                     /locus_tag="VF089_21060"
     CDS             4145..>4833
                     /locus_tag="VF089_21060"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176505.1"
                     /translation="MKLISAAFSALSLLWLLITSPQAAEKLVADYGGLSGFQSTVWVA
                     KDLKIFDKHGLDVDVIMINGSARSVAALLGGSTQFATGSATGPLVAAARGADVRVIAA
                     SYNKFPYAFIVKPEIRTPKDLRGKKVNILNFGGSNDLALQLALREWGLKLPDVQVIIG
                     GDAPTRLASLMTGRTDATILSPPHLTKAVQAGYRVLADMGEMSANFTQSSLYVKGSFL
                     REDRDRVKRFVR"
BASE COUNT         1092 a         1357 c         1303 g         1081 t
ORIGIN      
        1 gatcgtctcg agcctgaatg atgctctggt acgtcggctg gtttatcttg gcgaacacag
       61 tcttggcgcc ccgccggcgc catttgcctg gatcgtcttt ggctccgaag gacgactgga
      121 acaagcccta ctgactgacc aggacaacgc gttggtctat gagagcgagt ccgcagcggc
      181 gcgaagctat ttcacagcgc tagcgaacta tcttgtggac gcgctgattc aggtcggctt
      241 tccgccatgc gccggcgggt acatggcgac caactggtgc aagtcgttaa accaatggca
      301 ggaacttttt atccgccata tacaaacgcc cgaaccgcaa gcgctattag acagcgcgat
      361 cttcttcgac tttcgcgcgg tagccggtga tctctcattg gctccgttgg aaaaaatact
      421 cgcgggcgcg cgccatgaga aacttttctt gtatcacatg attcgcggcg ctctcgaatt
      481 tccgccgccg ttgggcttct tcaacagggt tcgcactgat aacggcaaag tggatctcaa
      541 aaaaggcggc atcgggccga tcgtatcttt ggctcgggcc gcggcattgt gcgccggttc
      601 acatgagcga ccaacgctcg caagactgga agcggccggc gcatcgggat cgctcctcag
      661 caacgaaaac gctcgcacgc tgagcgatat tttttctttc ctgttgcacc tgcgactgcg
      721 ccggcaactc actgcttatc gtcataaaga gcctctggat catgcgatca gcctggcaga
      781 gctctcgact ttggagcggc gccatttgca agaggcattc gtcatgatca agcggataca
      841 agacgcgctg atgcttgaca gtcattatcc atgacaatag attcgatgat caccaacaaa
      901 ggaaacgcct acgtcaaagc agcaacaaga atcgttgaaa ctcgtaccca tcgatgaggc
      961 gcatccgccc agctcgaacc tctggctggc gcgcttgcgt gaagttgtat ccaattggct
     1021 tgggatcatc ggcatcctga tattttttct tgcctgggag atcgcaaccc gcacgcacat
     1081 tctcaatcct ttttattttc cgccattttc gcagatcctc gccaaaggct acgagctttt
     1141 cgccaatggc tcgatctggg agcatatgcg gttcagcctg actaatttcg ccataggctt
     1201 tctgatctcg gtgatgctcg gcgtggtcat tggagttccg atgggctggt atcggagctt
     1261 aagcaagctt tttgatccgc tgctctccgg gatatacgcc acaccgctga tcgccttgct
     1321 gcccttgatc atcatgattt tcggcctggg tccggtatca aaaatcatca tgacgattct
     1381 cgcggcggtg tttccgatcc tgatcaatac catgactggc atcgccaaca ccgaccatcg
     1441 tctgattacg atggcacggg cctttggcgc caaggacagc acgatctttc tcaaagtctc
     1501 ggttccggga tcgcttcctt acattgtcgc cggcatgcgc gtggcgctgg gccgcgcact
     1561 ggtctacatc gtcgtcgccg agcaatacgg cgccgccacc ggtctcggct atctgagctc
     1621 agtggcggcg caacgctttc agatgtcggc aatgttcgtg cctatcgtta taatcgccgc
     1681 gctcggcgct agcttgaacg aactcttgaa ggtcctcgag caacggcttg ataaatggaa
     1741 accgcgcaag tgactacggc gcatcgaatc acctgaggag gcaacgcatg aattcggcaa
     1801 agatactgct ttgtgtctcg gttctcacct tgacggtcgt ggcagcatgg tccaacagca
     1861 gccgcgccca aggggcaaag atgaccgtcg gccagaccgg caccaatccc ggaacatcgc
     1921 ttttctttat tgcggaaaag gagaagctgt ttgccaagca cgggctcgac gtcaacatca
     1981 tcaagtccaa taccgcggcc gcggtgcagg cgatgctcag cggcagcatg catatggcca
     2041 cgggctcggg cgccgcggcg ttcacgaccg caacgatcga gggcgcgccg ccttttgttc
     2101 tggtaggctc ctgggtcaac gtgttcccgt acaagatcat ggcactcaaa gagatcgcca
     2161 aaatcgaaga tcttaaagga aagaccggtc acgtgggcgc accgttcggg acaatacccg
     2221 ataccgcatt gagatttgcc ctggcaaagc tgaaactgga cccggaaaaa gacgttaaac
     2281 tcgtccagaa cgccagtgcc gatccggcgg cgatcctagc ctccatggac aggggcgacg
     2341 tgcagttcgg cattctggct gcaccgttcg atcgcgtcgc ggaaaggcgc ggctatcgaa
     2401 cgctgctcgc cttgccggag ttgggaattc cctggcaaca gaacggtgag tgggttcagc
     2461 gctcttatct taagtcgaat cgcgacaatg tcgttcgctt catgaaagcg atatccgacg
     2521 ccatgaacat ctatttcggg caaaaggaaa aaaccgtcgc ttatttaacg gagttcatgg
     2581 gcagcaagcc cgaggatacg gagtatgcct acgaattgtt cgccaagtgg gccgaccgga
     2641 gtcccaggcc aaaagtcgaa accgttcgca cgaccctgga agccatcaaa aagaacactc
     2701 cgaaagcagc gagcgtcgat ccggcatcgt ttatcgacac cagcatcgtc gatcagctaa
     2761 tcaaagaagg ccatgtcaaa taagggcagg gaggtttgac cgggcacaac cgcgatgtta
     2821 gcatccgcgc agttctgtaa ggagtaaaat attccgtgaa gctctataat tttgatctcc
     2881 tggcttatcc ccatgttccc aaagacgcgc cgcgcacgcc ggtgccaagc aattttttcg
     2941 atccgattaa aggcgccgcc aactatgccg aacacctaga ggagatgacc tattgtgaag
     3001 agcttggcct ggacggcgtt gttttcaacg agcatcacta cagttcctac ggcaccatgc
     3061 catcgcccaa tctcattgcc gctgctctga gccagagaac caagcgaatc aaaatcggcg
     3121 tgctcggaaa tattcttccc ctgcgcaatc acccggtgcg ggttgccgaa gaatacgcga
     3181 tgatcgactg cctttcgaac ggccggttga tcgcgggctt cgtgcgcggc attcctgcgg
     3241 aatatgtttg gtatggtgtg aacccggaag aatcgcgcgg gcgctttcaa gaagcttatg
     3301 atctgatcat gaccgcatgg actaattcag tctggagctt cgagggagaa ttttacaagc
     3361 tcaaggattg cgccatctgg ccgcgcccgc tgcagcaacc tcacccgccc atttggattg
     3421 cggcgcgcag cgccgagtcg atcgaatggt gcgtcaagcg gcacctgcca tgtgcacagg
     3481 tgtaccagac tacggctcag atcgaagaca cgtttaacta ctatcgcacc aaggcgaagg
     3541 aggatggatg ggaagcccgg ccggacgatt tcatcttgtg ccgtcacatc tatatcgatg
     3601 agtcgaacaa gaaagctcaa gaggtcgccg aaccggcaat gcggtatttt ttctccgtct
     3661 ataatcgggg tttcaacgac gccatcaatc aaacggcggc cgatcaacag aaactcctgg
     3721 ccaagctcac cagcgaccgc tctttcaatt acttccgcga aggcaatcgt tcgcgcatcg
     3781 atttttcaac tctcggatgg gacgagttgg taaagagcgg ctacatgatt gccggcgacc
     3841 cggacagcgt tgcgcgccag attcaggatc agatgaagca gattggtgcg gaccatttca
     3901 tgggcatgtt ccacattggt aacttagctc atgagaaagt catttcttct ctcgatctct
     3961 tcaaaaaaga gatcatgccc agacttaact gacggctaag gattggacgt ttgcaaaaga
     4021 attcgagggt cttcccaccc gtcatgctcg cgaaagcggg catccagtac catttgattt
     4081 tcctggattc cgggtcgcgc taggctatcg ccagcttgcc cggaatgacg actcaggagt
     4141 taacatgaag ctcatctccg ctgcgttttc ggcattgtcg ttactatggc tgttgatcac
     4201 cagcccgcaa gcagcggaga agctcgtcgc cgactatggc gggctttccg gttttcaaag
     4261 caccgtgtgg gtggcaaagg atctgaaaat attcgataag cacggcctgg atgtcgatgt
     4321 gatcatgatc aacggcagtg ctcgttcggt ggccgcactg ctgggcggca gcacccaatt
     4381 tgccacaggc tcggctaccg ggcctctcgt agccgccgct cgcggggcag acgtaagagt
     4441 catcgccgct tcgtacaaca aatttcctta cgcctttatc gtcaaacccg aaattcgcac
     4501 gcccaaagat ttgcgcggca agaaagttaa catcctcaac ttcggcggtt ccaatgatct
     4561 tgctctgcag ctggcgttaa gggaatgggg tttgaagctg ccggatgttc aagtcatcat
     4621 tggcggcgat gcgccgaccc gtcttgcatc cctcatgacc ggccggaccg atgccacaat
     4681 actttcacca ccacatttga ccaaggcagt ccaggcgggc tatcgggtgt tagccgacat
     4741 gggagagatg agcgctaact tcacgcagtc ctctctctac gtcaaaggca gttttctccg
     4801 ggaagaccgc gatcgggtca agcgtttcgt ccg
//
LOCUS       DASUTG010000907         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_437606, whole genome shotgun sequence.
ACCESSION   DASUTG010000907 DASUTG010000000
VERSION     DASUTG010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437606"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..>2064)
                     /locus_tag="VF089_21065"
     CDS             complement(203..>2064)
                     /locus_tag="VF089_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176506.1"
                     /translation="SGISTIDFGSVAPRKGFGMYRPPAGLTTGGITFRAAGGARFGPG
                     IIYVPSVHYMGTNPLFQTGTGEMIAWGAPNQPGNASLDVTLPPGVTAIGVDLWTQQPY
                     VSSIEVIATTEDGQSETVVVNTRNRPDAAFVGFIAEKSIVSISFRPPKGQTGLLLDNF
                     SFGRRADGRSVRASNRPTTPAVSEVAQTKATSTTPSVEPRVETTNPKPTQTPTTSTPR
                     PAQSSSTTPGPGTIAYVRGGTEIRLIESDGTNDRRLWTHQYSQEGAGIYELSWRPDGK
                     ELAFSSAHLNVASLYHSDVYAIGADGKGYRKITNNPDHGELTRYRKGAVTVTVRNDQP
                     AWNRSQASTGVFFVYVVGADEPQSITLPPGSSKTLTFKSVADYGDHAQAVVAMYGSHR
                     WFIPGLDVRAGQTTKAPDFSISGDGIELFGAFRPTWRADGSRISYRSGLCVTSSISSQ
                     PNPGEFVFKPLFSGEPPSGACTWDWGPTPALADQILYTDNTAGSHIYRLKEGGTHPGT
                     KIHTFSDLNHQILLDLKWLPDGSGFLYAYPDLAYEFGNIFRYDFGSKRVTQLTDYKDM
                     NARAFDISPDGQWIVYERSKKWNDDKGVELWIMRVDGSNQRMLVRDGTSPSWR"
BASE COUNT          457 a          569 c          561 g          477 t
ORIGIN      
        1 ccgcggcttg ctggcccatc cccggcgagg cgtacgttgc cgagctcacc agtgcaaaaa
       61 ctatcattgg gatcgtttta aacttggtag acacgggttc atcctccatc aaaaacatca
      121 atgccattta tttcgtgtaa ttctgtgcga ttgttttatg gtcgcgtcta aacacgttca
      181 cgaatcacac gaaatatacc gcctagcgcc aggacggcga cgtgccatca cgcaccagca
      241 ttcgctggtt acttccgtcg acccgcatga tccaaagctc aactcccttg tcgtcattcc
      301 acttcttcga tcgctcgtaa acgatccatt gaccgtccgg cgaaatatcg aacgcacgag
      361 cattcatgtc tttgtaatca gtcaactggg tgacgcgttt cgagccgaag tcgtagcgaa
      421 agatatttcc gaactcgtag gcgagatcgg gataagcata aaggaaaccg gaaccgtcag
      481 gtaaccactt caagtccaga agtatctgat gattaaggtc gctgaaggtg tgtatttttg
      541 ttcccggatg ggtgccacct tccttaaggc gatatatgtg tgaccctgcg gtgttgtcgg
      601 tgtacaggat ctgatctgca agcgccggtg tcggccccca gtcccatgta cacgcacctg
      661 acggcggctc accggaaaac agtggtttaa atacaaactc acccggattc ggttgggaag
      721 aaatgctgct ggttacgcag agaccggaac gataactaat tcttgagcca tcggcgcgcc
      781 acgtcggacg aaacgctccg aatagttcga tgccgtcacc gctgatacta aaatccggcg
      841 ccttggtggt ctgccccgcg cgaacgtcga gtccgggaat aaaccaacga tgacttccgt
      901 acatcgcaac gaccgcttgc gcgtgatcgc catagtccgc gacagattta aacgtcagcg
      961 tcttcgagga gccgggcgga agtgtgatcg actgaggttc atcagcgcca accacgtaaa
     1021 cgaaaaagac gccggtactg gcctgcgatc gattccaggc cggttgatcg tttcggacgg
     1081 taaccgttac cgcccctttc cgatagcgtg tcagctcgcc gtgatcagga ttatttgtaa
     1141 tcttgcgata acccttgcca tcagcgccga tcgcatagac atccgagtga taaagtgacg
     1201 ctacgttgag atgagcactc gaaaaggcga gttccttccc gtctggtctc cacgagagtt
     1261 cgtaaatgcc ggcgccttcc tgcgagtact gatgcgtcca aagccggcga tcgttggtgc
     1321 cgtcggattc aatcaatcga atttcggtac cgccgcgaac gtatgcgatc gtcccggggc
     1381 ccggtgtggt ggaggaactc tgcgccgggc gtggtgtact ggtcgtcggc gtctgggttg
     1441 gcttcggatt cgttgtttca acacgcggct caacagatgg agttgttgat gttgctttcg
     1501 tttgtgcgac ttcgctcacc gccggcgttg ttggtctatt tgatgcccgc actgatcttc
     1561 catcagcacg ccggccaaag ctgaaattgt cgaggagcag tccagtctgg cctttcgggg
     1621 gacgaaaact gattgagaca atcgactttt cggcaatgaa accgacgaag gccgcgtcag
     1681 gacgattgcg tgtgttcacg acaacggttt cactctgccc atcttccgta gttgcgataa
     1741 cttcaatcga ggacacgtac ggctgttgcg tccacaagtc gacgcctatt gctgttactc
     1801 ccggaggcaa tgtgacgtcg agcgaagcat tgccaggctg attgggtgct ccccaggcga
     1861 tcatttctcc ggtaccagtc tggaaaagcg ggttcgtccc catgtagtga acactcggca
     1921 cgtagataat gcccgggcca aatcgcgccc cgcccgcagc ccgaaaagtg ataccgccag
     1981 ttgttaatcc cgctggcggc cggtacatac cgaagccctt acgcggagct actgagccga
     2041 agtcgatggt ggaaatgccg gacg
//
LOCUS       DASUTG010000908         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1177065, whole genome shotgun sequence.
ACCESSION   DASUTG010000908 DASUTG010000000
VERSION     DASUTG010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177065"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF089_21070"
     CDS             <1..493
                     /locus_tag="VF089_21070"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176507.1"
                     /translation="TYAFSGSDGLPFAIHLSSPQKFWEGLCKSAGHPEVIDDPRFSTR
                     PNRRKNYDELNKVFAEFFKEKPRSYWLEQLEANDVPHTPVYNMLELFEDPQIKHMGLE
                     IKIDRKDKPDIRTVRFPNEYSATKSPHPAPPPELGEHNAEFLTALGYDDKKIAEFKDK
                     GVI"
     gene            595..1770
                     /locus_tag="VF089_21075"
     CDS             595..1770
                     /locus_tag="VF089_21075"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6176508.1"
                     /translation="MRRYDVAIIGGGAAGLMCAIEVGKRGRRVLIIERSGQIGKKILI
                     SGGGRCNFTNLHVTAENFLSANPHFCKSALARYSPADFIAMVERHGIAYHEKERGQLF
                     CDLSAKLIVTMLADECRQAGVRIDVNGVVTSVEREGEGFTIETGHGPTHAGSLVIASG
                     GLSIPKMGATNFGYELAKRFGHTIVPTRPALVPLTFSADDLARYQELSGIGLPVEASC
                     NGESFAAPMLFTHRGLSGPAILQISSYWRLGEELRVNLLPDIDAAEWLLERHQSRPAA
                     ELKSVLGERLPKRLAQRLSERFGTRPLRQYRLAELRHVAAQLQSWAFHPVGTEGYRTA
                     EVTAGGVNTDELSSTTMESKKMPGLYIIGEVVDVTGHLGGFNFQWAWASGHAAGQVV"
     gene            1881..3440
                     /locus_tag="VF089_21080"
     CDS             1881..3440
                     /locus_tag="VF089_21080"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX6176509.1"
                     /translation="MKMILGPILGFRGVSTSGWNVSALVVTDVVGDPPEAIVQSGNKK
                     GTKRAARLLKAFPESNPTHQVWRFDFAVRQTARAQPVSYSIGGVENAFVVPPRDTIPN
                     MLYASCNGFSSMRLLNDTQDANRLWRHAAEKHEARPYHLLMMGGDQLYCDSMWDQLAP
                     LHEWARLPLDQRIKQPFTNEMRSLVDQFYAQVYIERWSQAEPARVFAQVPSIMMWDDH
                     DIFDGWGSYEAELQQCDVYKGIFAAAREYFALYQLQLARDEIHPLSIKAQEAFSLGAQ
                     IGGLTLLALDMRSERTKSQVISGQSWKAIYDWLDAISPRPRSQTPSARHLLLMSSIPV
                     VHPEFALLEKLLGIFPGRQQLEDDLRDQWLSVPHRQERLRLVHRLLAFADAKKCRVTV
                     LSGDVHVGALGIIESARSTADDPSTQVINQLTSSGIVHPPPPGVVLYFLENVTGKKME
                     LDRDLYSEMLEFPGTHHHYIGARNWLSLEPDDSGRIWANWHVETEPYPYTKVIHPIDF
                     RMPAPIVASQP"
     gene            3525..4139
                     /locus_tag="VF089_21085"
     CDS             3525..4139
                     /locus_tag="VF089_21085"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX6176510.1"
                     /translation="MNLHVTNGDATRKVMEKAGLGGEVLPWRDVLHEGPVPAGLSLAQ
                     MSEVRAQFIADCGWQTREKAWADFRARDTKLASFRKYQEVILWFEHDLYDQLQLLQIL
                     DWFAGRDFNDTALSMICAGDYLGTMQPESVAALYTLRQPVLPEHLELGVKAWSAFCSP
                     DPMCWEDLLAMDSAALPFLGASVVRQSERNCEVHETPSRSGRYP"
     gene            4277..>4398
                     /locus_tag="VF089_21090"
     CDS             4277..>4398
                     /locus_tag="VF089_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX6176511.1"
                     /translation="MVSSSERNWRDHGVKIIPSAQLDLNTVQTPGMIRASAITHA"
BASE COUNT         1013 a         1207 c         1225 g          953 t
ORIGIN      
        1 aacttacgcc ttcagcggct ctgacggctt gccattcgcc atccatctct cgtcgccgca
       61 gaaattttgg gaaggtctgt gcaagtccgc gggccatcct gaagtgatcg atgatccgcg
      121 ctttagcact cgaccgaacc gtcggaagaa ttatgatgaa ttaaacaaag tctttgccga
      181 attcttcaaa gagaaaccgc gctcctattg gctcgaacag ctcgaggcta acgatgtgcc
      241 gcacacgccc gtttacaaca tgcttgaact gttcgaagac ccgcagataa agcacatggg
      301 tctcgagata aagatcgacc gcaaggacaa gccggacatc cgcacggtta gattccccaa
      361 cgaatacagc gcgaccaaat cacctcatcc cgcgccgccg cccgagctcg gcgagcacaa
      421 tgcggagttt ttaacagcgc tgggatatga cgacaagaag atcgcagagt tcaaagacaa
      481 gggcgtgatt taaccaacgg cagcaatcgg cataagccat ctgcaaatgc cgattgcctc
      541 atctgcaggc tttaggaatg attttgtcgg ccgccgcccc tctcatctcg atcgatgcgc
      601 cgttatgatg tagccatcat tggcggcggc gccgccggtc tgatgtgcgc catcgaggtg
      661 ggcaaacgcg gacggcgcgt tctgatcatt gagcgcagcg gccaaatcgg caaaaaaatc
      721 ctcatctcgg gcggcggccg ctgtaacttc accaacctgc atgttacggc ggaaaatttc
      781 ttatcggcca atccgcattt ctgcaaatca gcactggctc gttatagtcc cgcggatttc
      841 atcgccatgg tggagcgcca tggcatcgcc tatcacgaaa aggagcgggg ccagttattt
      901 tgcgatctct cagccaagct gattgtgacg atgctggccg atgaatgccg gcaagccggc
      961 gtgcgcatcg acgtaaacgg cgtggtgaca tctgtagagc gagagggcga gggttttaca
     1021 atcgagaccg gccacggccc gactcacgcc ggatcgctag tcatcgcttc aggcggtcta
     1081 tcgatcccga agatgggcgc tacgaatttc ggttatgagc tcgccaaacg atttggacac
     1141 acgatcgtgc cgacccgtcc ggcgctggtg ccgctcacgt tcagcgcaga tgatctggcg
     1201 cgctatcaag aactaagcgg cattggatta ccggtcgaag caagttgtaa tggtgaaagt
     1261 tttgccgccc cgatgctgtt tacccatcgc ggcctcagcg ggccggcgat tttacagata
     1321 tcttcttact ggaggctcgg agaagagcta cgcgtgaatc ttttgccaga tatcgacgcg
     1381 gcggagtggt tgctggagcg gcaccagagc cggccggcgg cggagctcaa gagcgtgctt
     1441 ggcgaaagac ttcccaaacg gctcgcccag agattatccg aaagattcgg tacccggccg
     1501 ctgcgacagt atcgcctcgc cgaattgcgg cacgttgccg cccagctaca gagctgggca
     1561 tttcatccgg tcggcacgga agggtatcgc accgcggagg tgaccgcagg cggcgtcaac
     1621 accgatgagc tttcctcgac aacaatggaa tctaaaaaaa tgcccgggct ttatataatc
     1681 ggcgaagtcg ttgacgtgac cggccatctg ggtggattca attttcaatg ggcttgggca
     1741 tccggccatg ccgcggggca ggtagtctag aaatcacgtt caaaagtcca gcggttcaaa
     1801 ggttcaacgt cagagttcga aatcggataa tcagcagaga tcaagacgag cgaatattct
     1861 tttcgtagag gagaccacgg atgaaaatga ttctcggacc tattctcggt tttcgcggcg
     1921 tcagcaccag cgggtggaac gtaagcgcgc tggtcgtgac cgatgtcgta ggcgatccgc
     1981 ctgaagcgat tgtccagagc ggcaacaaga aaggaaccaa acgcgcggca agacttttga
     2041 aagcgtttcc cgagtccaat ccgactcacc aggtctggcg tttcgatttc gccgttagac
     2101 agactgcccg cgcgcagcca gtcagttaca gcatcggcgg cgtggaaaat gcttttgtcg
     2161 tgccgccgcg cgacaccatc cccaacatgc tttacgcatc ctgcaatgga ttttccagta
     2221 tgaggttgct gaacgacacc caagacgcca accggttatg gcgccacgct gctgagaagc
     2281 atgaagccag accctaccat ctattgatga tgggcggaga tcaactgtat tgcgattcaa
     2341 tgtgggacca gcttgctccg ctccacgaat gggcgagatt gccgcttgat caacggatca
     2401 agcagccgtt taccaatgaa atgcgctcgt tggtggatca gttctatgcc caggtctaca
     2461 tagagcgatg gtcacaagcc gagcccgctc gcgtttttgc tcaagtgccg agcatcatga
     2521 tgtgggacga ccacgatatc tttgatggct ggggatcgta cgaagcggag ctccaacaat
     2581 gtgatgttta caaaggaata tttgctgctg cgagagaata ttttgcgctc taccagttgc
     2641 aacttgcccg cgacgagatc cacccgctgt cgatcaaggc gcaggaggcg tttagcctcg
     2701 gcgcccagat aggcggcctg acactgctgg ccctggacat gcgaagcgaa agaaccaaga
     2761 gccaggtcat cagcgggcaa tcctggaagg ctatttatga ttggttggat gcaatctcgc
     2821 cgcgcccaag gagccaaacc ccatcggcgc gacatctgtt gctcatgtcc agtattccag
     2881 tggtccatcc tgagttcgcc ctgctcgaga agctcctggg gatttttcct ggacggcaac
     2941 agctggaaga cgatctcaga gatcagtggc tgagcgtacc tcatcgccaa gaacggttgc
     3001 ggctggttca ccgattgctg gccttcgccg atgctaagaa atgccgagtc accgtgcttt
     3061 ccggcgatgt gcacgttggc gctcttggaa tcatcgaatc ggcccgttcg actgcagatg
     3121 atcccagcac ccaggtgatc aatcaattga cgtcctcggg catcgtgcat ccaccgccac
     3181 cgggcgtggt cctctatttt ctggaaaatg taaccggcaa gaagatggaa ctcgaccgcg
     3241 acctttacag cgagatgctg gagtttcctg gaacccatca tcattatatc ggcgccagga
     3301 actggctcag cttagaaccc gatgattcag gaagaatctg ggcgaattgg cacgtcgaaa
     3361 cggaacccta cccgtatacc aaagtcattc atccgatcga tttcagaatg ccggcgccga
     3421 tagtggcgag ccaaccgtag ttcgcgacgc tgttgttacg gacaataaat gaattatctt
     3481 ctcttgggtt gccgtttccg gcgcgctcgc tggtataacc accgatgaac ttacacgtca
     3541 ccaacggcga tgccactaga aaagtcatgg agaaagccgg cctcggcgga gaggtcttgc
     3601 cgtggcgtga tgtattgcat gaagggccgg tacccgccgg gctttccctt gcccagatgt
     3661 cggaggttcg cgcccaattc atcgccgatt gcggctggca gacgcgtgag aaagcatggg
     3721 ctgactttcg agctagagat accaagctag cttcttttcg aaaatatcaa gaagttatcc
     3781 tctggttcga gcacgacctg tacgatcagc ttcagctgtt acagatactc gactggtttg
     3841 ccggtcgaga tttcaacgac actgcgctca gcatgatttg tgctggcgac tacctgggca
     3901 cgatgcagcc ggaatccgtc gctgcgcttt atacactacg tcaacccgtg ctaccggaac
     3961 atctggagct tggcgtgaaa gcttggtcgg cgttctgttc tcctgacccc atgtgctggg
     4021 aagatttgtt ggcgatggat tcagcggctt tacctttcct cggcgcatcg gtagttcggc
     4081 agtccgaacg aaattgtgaa gtccacgaaa ctccctctcg gagcggtcgg tacccatgat
     4141 gtctctgttg tcagtttgag cgcagcgaga actcttagac cccgcattca gtcggattta
     4201 cattaggatc ctggagcagg agatgggcct ctgccggaaa ccgaacagtg aggttaacga
     4261 acgaacggag gaagatatgg tttcatcatc cgaacggaat tggcgagacc atggcgtcaa
     4321 aatcattccg agcgcgcagc tcgatctcaa taccgtgcaa actcccggga tgatccgcgc
     4381 ttcggcgatc acccacgc
//
LOCUS       DASUTG010000909         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1922542, whole genome shotgun sequence.
ACCESSION   DASUTG010000909 DASUTG010000000
VERSION     DASUTG010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922542"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF089_21095"
     CDS             <1..478
                     /locus_tag="VF089_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6176512.1"
                     /translation="KLKSAGAALKAIERAKAPFVSRGDNSGTHNLEKSLWKAAGIQPS
                     GSWYIESGQGMGATLGIAQERRAYTVTDRGTYLALRRRVTLPILLEGDRVLMNIYSVM
                     EVNSANSPRVNAAGGKAFADFIVSPQVQNLIKSFGIDKFGQALFVPIAQMREEEGA"
     gene            480..1193
                     /locus_tag="VF089_21100"
     CDS             480..1193
                     /locus_tag="VF089_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176513.1"
                     /translation="MELIWHGFLKAVELVLGLDREVWSITLRSLQISGAATLISLLIG
                     MPLGIALALARFPGRGTTVALINTGMGLPPVVVGLFISIFLWRSGPLGFLELIYTPAA
                     MIVAQVVIAFPIVAGLTMASFQALDPKLALQLLGIGASKSQLLWLLCKEAKLPLLAAV
                     MAGFGGVISEVGASMMVGGNIRGQTRVLTTATVLETSKGNFEIAIALGLILLALTFAI
                     NLLLTRIQQREQLRWPRLS"
     gene            1175..2260
                     /locus_tag="VF089_21105"
     CDS             1175..2260
                     /locus_tag="VF089_21105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176514.1"
                     /translation="MAATLLTLRDVIVRYGETIALQIAALELQSGEVLAILGPNGGGK
                     STLLRVIGLLQRPTHGKVLFRGENAFDGNSLRLRRHIASVFQEPLLLNATVYENAALG
                     LKLRGIGGPEISRRLDPWLERLGIADLRQRNARSLSGGEAQRTSLARALALEPEILLL
                     DEPFAALDPASREALLRDFEPIVNENKITTVFVTHDRNEAFGLAGRIAVLREGRVAQI
                     GAREEVFDRPANEAVADVVGIENRLSGVVEDCAGGLASIRIKQIRLRVAGQFEPGARV
                     VLCLRPESITISRGNCPAENSNRLTGVIIKGVSPGMLQQRITLDRDGITFVALIERQT
                     CVNLKLAEGEMVTITFRFSAAHIITSH"
     gene            complement(2398..>3212)
                     /locus_tag="VF089_21110"
     CDS             complement(2398..>3212)
                     /locus_tag="VF089_21110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6176515.1"
                     /translation="FRDRGAGNPIVFIHGFGASLDTWRYLEERLKGEYRIVSLDLKGH
                     GYSERPLDDRYSLQDHAAAVLGLMDHLKLKNVVLVGNSLGCAVALIAALEAQQESPAT
                     VAAMVLIAGSLDGDNLPFYLRLLRLPMIGSVAAKLTPASFGTRLILKRAYYDDEKVTD
                     SLVELYAKYQRIPGTEHALITTARRMIPANSSGLREALKKLEIPILNIIGKHDQIISR
                     ESSEGVCQILRRCKDVTIDEAGHVPHEEKPEEVIPLLKDFLAEVFGLRSKRL"
BASE COUNT          669 a          904 c          915 g          724 t
ORIGIN      
        1 aaagcttaag tccgcgggcg cggctctcaa agcgatcgag cgcgccaagg caccgtttgt
       61 ctcccgcggc gacaactccg gcacgcacaa tcttgaaaag tctttatgga aagccgccgg
      121 catccagccc agcggctcct ggtatatcga gtcgggtcag ggcatgggag cgactctcgg
      181 gatcgcacag gaacgccgtg cctatacagt caccgaccgc ggcacttatc tggcgttacg
      241 acggcgtgtc acgctaccga ttctcctgga aggcgatcgc gtgctaatga acatatattc
      301 cgttatggaa gtgaactctg ccaatagtcc gcgtgtgaat gccgcgggag ggaaggcgtt
      361 cgcggatttc atcgtgtcgc cgcaggtgca gaatctcatc aaaagctttg gcatcgacaa
      421 gttcggtcaa gctttgtttg tacctatcgc ccagatgcga gaagaagagg gggcatgact
      481 tggagctgat ctggcacggt tttctcaaag ctgtggagtt ggtccttggt ctcgatagag
      541 aagtatggtc catcaccttg cgctcactcc agatctccgg cgccgcaact ttaatttcac
      601 ttttgatcgg tatgcccctc ggtattgccc tcgcacttgc gaggtttccg ggccgcggta
      661 ctaccgtagc tctgataaat accggtatgg gtcttccacc ggtggtggtc ggtttgttta
      721 tctcgatttt tctctggcgc agcggcccgc taggcttcct ggagctgatc tacacgccgg
      781 cggcgatgat cgttgcgcag gtggtcattg cttttcctat tgtcgcaggc ttaacgatgg
      841 cgagctttca agcgctcgat ccgaagctag ccttgcaact gctcggcatc ggcgcttcga
      901 aatcgcagtt gctctggctg ctctgcaaag aggcgaagtt accgctgttg gcggcggtca
      961 tggcgggctt cggtggcgtg atttcggaag tcggcgcctc gatgatggtc ggcggcaaca
     1021 tacgaggcca aacgcgggtc ctcacaacag ccaccgtgct ggaaacaagc aaaggcaact
     1081 tcgaaatcgc cattgcgctg ggactgatcc tactggcgtt gaccttcgcc atcaacctct
     1141 tactcacccg tatccaacag cgggaacagt tgcgatggcc gcgactctcc tgactctgcg
     1201 cgatgtgatt gtccgttatg gcgagacaat cgcgctgcag atcgctgccc tcgagctgca
     1261 gagcggcgag gttttggcaa tcctcgggcc caatgggggg ggcaagtcca cgttgttgcg
     1321 ggttatcggt ttgctccagc gcccgaccca tggcaaggtc cttttccgag gcgagaatgc
     1381 atttgatgga aattcgctac gcctcaggcg gcatatcgcg agcgtctttc aggagccact
     1441 gctactcaat gcaacggttt acgaaaacgc ggctcttggg ttgaagctgc gcggtatcgg
     1501 cggccctgaa atttcacggc gcctcgaccc gtggctggag cggcttggta tcgccgatct
     1561 gcggcaacgt aacgcgcgca gcctgtccgg cggcgaggcg caacgcacca gtttggcccg
     1621 cgcgcttgcg ctcgaacctg agattctgct gcttgacgag ccgtttgcgg cgctggaccc
     1681 tgccagccgc gaagctttac tgcgtgattt tgagccgatt gtgaacgaga acaaaataac
     1741 aacagtcttt gtcacccatg accgcaacga agccttcggc ctggccggtc gcatagcggt
     1801 tttacgcgaa ggtcgcgtcg ctcagatcgg cgcgcgggag gaggtctttg atcgaccggc
     1861 caacgaggcc gtggcggacg tcgtgggaat tgagaatcgc ctatccggtg tggtcgagga
     1921 ctgcgccggc ggcttggcga gcattcggat caaacagatc aggttaagag tcgcgggaca
     1981 attcgagccc ggcgcgcgcg tcgtcctgtg cctgcgcccg gagagcatta cgatcagccg
     2041 gggcaattgc cccgcagaga attccaaccg actcacgggc gtaatcatca aaggcgtttc
     2101 gcccggtatg ttgcagcaac gcatcacttt ggaccgcgac ggaatcacgt ttgtagcgct
     2161 gatagaacga caaacttgcg ttaacttgaa actggcggaa ggagaaatgg tcacgattac
     2221 gttccgcttc agcgcagcgc atatcatcac ctctcactga tgaggatccg caccgatact
     2281 ctcagcggat tcagcacgtt cttgtcgggc ttgaaactga ttttcgccga aacacctgat
     2341 ccctgacatc tctctgtctg gccagcgatc gatcctcggt gagatccgaa ttaaaattca
     2401 taagcgttta ctccggagac caaacacttc agcaaggaag tccttcaaca gtggaattac
     2461 ttcttccggc ttttcttcat ggggcacatg accggcctca tcaatggtga cgtctttgca
     2521 gcgccgcagt atctgacaca cgccctcgga actttcacgg gaaataattt gatcatgttt
     2581 gccgataata ttcagtatcg gaatctccag tttttttaat gcctcccgca gccctgagct
     2641 attggcggga atcatccgtc gagctgtggt tatgagggcg tgttccgttc ccggtatccg
     2701 ttggtattta gcatagagct ctaccagtga gtccgttacc ttctcatcat cataataggc
     2761 gcgtttcagt atcagccgag taccgaaact agcgggcgta agctttgccg caaccgaccc
     2821 aatcataggg aggcgcaaaa gccgcaggta aaaaggcagg ttatccccat cgaggctgcc
     2881 agctatcagg accatcgccg cgacggtcgc gggagattct tgttgcgcct ctagagccgc
     2941 tatcagagca actgcgcaac cgagagagtt gcccacgagc actacattct taagcttcag
     3001 atgatccatc agccctaaca ccgcggcggc atgatcctgc aaagaatagc gatcgtccag
     3061 cggtctctcg gaatagccgt ggcccttgag atctagagag acgatccgat attcgccctt
     3121 gagccgctcc tcgagatagc gccaggtgtc gagcgaagcg ccgaacccgt ggataaagac
     3181 aatcgggttt cccgcaccgc ggtctctgaa gt
//
LOCUS       DASUTG010000910         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_586035, whole genome shotgun sequence.
ACCESSION   DASUTG010000910 DASUTG010000000
VERSION     DASUTG010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586035"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..485
                     /locus_tag="VF089_21115"
     CDS             39..485
                     /locus_tag="VF089_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6176516.1"
                     /translation="MSAHTIRLHRVLRATPERVYRAFLDSDAMAKWLPPNGFTGRVHH
                     MDARVGGTYKMSFTNFTTGKSHSFGGEYLELVPQERIRFTDRFDDPGLPGEIQTAITL
                     KKVSCGTELNVVQEGVPTAIPAEACYLGWQESLALLAQLVEAEIPN"
     gene            788..1771
                     /locus_tag="VF089_21120"
     CDS             788..1771
                     /locus_tag="VF089_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176517.1"
                     /translation="MLRLTLAAGHYDRTEALRDGRVRAEGIDLTYIMLRVEEIFWRMA
                     QHREFDASEFSLGGYVVRRGRGADDLVAIPVFPSRFFRQSCIFVHDGSGVRGPEDLRG
                     KRIGVPEYQMTAAVWARGILADDYGVRAGNVEWVQGGLEEPGRIPFEPAEPPGVMVGF
                     APADRSLAQMLESGEIDALITARTPSTFRRSGTAVRRLFEQPFEVERDYFRRTGIFPI
                     MHTLVVKKEILDANPWVAFTLFKAFSEAKDSAMADLTQTSALPLSLPFLVEHAYQTMD
                     LMGEDFWPYGLESNRHTLETFVRYMHEQGLIPEPMALETLFPASTQRTFRV"
     gene            complement(2216..2848)
                     /locus_tag="VF089_21125"
     CDS             complement(2216..2848)
                     /locus_tag="VF089_21125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEX6176518.1"
                     /translation="MNLIDRNDACTVTSLDDLAKIYAKPLPHVATKETDHITEVGRAF
                     IAASPFLVLATSNGTNVDCSPKGDAPGFVQLFDDRTLLIPDRPGNNRIDGMKNLLVNP
                     KVGIIFMVPGSNETYRVTGSATISTDPELLKRFEVSGKPPRVVMVVAVEEAFNHCPKA
                     LVRAKLWEATALPSNAPTHGDFAAARDGKDAAYARSYNEKYAERLKTELY"
BASE COUNT          725 a          960 c         1024 g          711 t
ORIGIN      
        1 aaggtctcaa caacacacaa caccgaaagg agaaaatcat gtcagcacac actatccggc
       61 ttcaccgcgt cctccgcgca acacccgaaa gagtgtatcg ggcgtttctt gatagcgacg
      121 caatggccaa atggcttccg ccgaacggtt tcaccggaag ggttcaccac atggatgcca
      181 gggtcggcgg cacctataag atgtcgttca cgaacttcac cacgggcaag agtcactcct
      241 tcggcggaga gtaccttgaa ttggtgccgc aggaacgtat ccgtttcacg gatagattcg
      301 acgacccggg tcttccggga gaaatccaga cggcaatcac cttaaagaaa gtgtcttgtg
      361 gaaccgagtt gaacgtcgta caggagggag tgcccactgc cattcccgcc gaggcttgct
      421 acctcggctg gcaggagtcg ctcgccttac tggcgcaact ggtcgaggcc gagattccaa
      481 attagtggag caggatttaa gacagagttg ggtccgcgta ggataatgcg atgatgccac
      541 gcgccaatcg gccacttctt ctcggccgta cctggcacat cactcgccgg taatggagta
      601 aaccctacgc gcacgaattt gccggcaaaa ataacgcttc aagcgtcaaa tacgatcgct
      661 tgaatgtaaa ctctgccggt agtgagaaaa gcgcggtccg acctatgata ctggatcaac
      721 atggaatgaa cttgaaatta acgtcacaag aaaatcagag caggattacc tgcgcccgga
      781 ggtggacatg ctgcgcttga ccttggctgc gggccactat gatcggacgg aggcgcttcg
      841 cgacggtcgg gttcgggcag agggtatcga cctgacctac atcatgctgc gcgtggagga
      901 gatcttctgg cgcatggctc agcaccgcga gtttgacgcc tcggagttct cgctcggcgg
      961 gtatgtagtg cgccgcggtc gcggcgccga cgacctcgtc gcgatcccgg tttttccgtc
     1021 ccgcttcttc cgccagtcgt gcatcttcgt ccacgatggc tcgggtgtcc gcggcccgga
     1081 ggatcttcgt ggcaagcgca tcggcgtacc cgagtaccag atgaccgccg ccgtgtgggc
     1141 gcgcggcatc ctggcagacg actatggggt gcgcgcgggc aacgttgaat gggtgcaagg
     1201 cggtctcgag gagcccgggc gcattccgtt cgaacccgcc gagccgccgg gagtcatggt
     1261 gggcttcgcg cccgcggacc gctcgctggc gcaaatgctc gagagtggag agatcgacgc
     1321 gctgatcacc gccaggacgc cgtcaacgtt tcggcgaagt ggtacggcgg tgcggcggct
     1381 cttcgagcag ccgttcgaag tcgagcgcga ctatttccgc cgaaccggca tttttcctat
     1441 catgcacacc ctggtggtga agaaagagat cctcgatgct aacccctggg tggcgtttac
     1501 gctgttcaag gcgttctcgg aagccaagga ttcggcgatg gccgacctga cgcagacaag
     1561 cgcgctgccg ctcagcctac cgttcctggt cgagcacgcc tatcagacca tggacctgat
     1621 gggcgaggac ttctggccat acgggctcga gagcaatcga cacacgctcg agacgttcgt
     1681 gcgctacatg cacgagcaag gactcatccc ggagccgatg gcgctcgaaa ccctgttccc
     1741 ggcgagcacg cagcggacgt tcagggtgtg acacgagcgg aaaagagaga tgccacaggg
     1801 gctacgggat agaggaagaa ttggtaccgg ctccacgaga tcgcgacgcg tctcggagtg
     1861 cattatgcga cagcgaaaca gcttagacgg atggcggagc gaagaacgct tgtgtggttg
     1921 caagacctgc ttccggagcc gattgcgtag ttggggatgg cagtaagcgt gaaaagtata
     1981 gtcgtatttc tcccgataca cgccacaggt gcctatagac ctggtagtta ctctccgaag
     2041 tgaaggtctt ctgccctgct tgcctcgcac aatgcccgca gcactacgca aggcaactcc
     2101 cggtgaaagc atcgctccgg ctggacgctg actgcggatc ggtcgttgat ctcttaagat
     2161 agtaaagtta tcgaagtctg accggtaact tgacgccgct cgtttcgcag cccgatcagt
     2221 aaagctcagt cttcaggcgc tcggcatatt tctcgttata actccgggcg taggcagcat
     2281 ccttgccgtc gcgggccgcg gcgaagtcgc cgtgcgtcgg ggcgttgctc ggcagcgcgg
     2341 tcgcctccca aagcttggcg cgcaccaggg ctttgggaca gtgattgaaa gcttcctcga
     2401 cagcgacaac catgaccacg cgcggcggct tgccgctcac ctcgaatcgc ttcagcaact
     2461 ccgggtccgt gctgattgtg gcactgccag tcacgcgata ggtttcgttg gatccgggaa
     2521 ccatgaaaat gataccgacc ttcgggttga cgagcaggtt cttcatcccg tcgatgcggt
     2581 tgttgcctgg cctgtcggga atcagcagcg ttctgtcgtc gaacagctga acgaagcccg
     2641 gggcatcgcc cttgggcgaa cagtcgacgt tggtcccgtt cgaggtcgcg agcaccagga
     2701 acggcgatgc cgcgatgaag gcgcgcccga cttccgtgat gtggtcggtt tctttagtgg
     2761 cgacgtgggg caacggcttg gcataaattt tcgcgagatc gtccagcgaa gtaacggtgc
     2821 aggcatcgtt ccggtctatc aaattcatgt catactcctc ctttgtcggt gacgggggtt
     2881 gagtcgtttt cgcagcatac aatatcgttt acccagccga gtcttgtcaa cattggaacg
     2941 tgatccacct ttttcacact cacgcccacg agcacgcatc acgatcacga gttttatctc
     3001 aggcccttat cccttatcct tgcgccctta ttctttcgaa catcctttgc ccttcgcatc
     3061 acgcaagagc tctcttgaac gacggttgat ccggagctac ttgcataaag aggagcgtct
     3121 gaacgattgg aacgactgta actgagtgac attggagact gctggcgaag tcgttcgcgg
     3181 agggtatagc atcaaatcga tgaaatcgaa tctccggaag atcgtatatc ggccgggatc
     3241 ggcattgatc gtgggaatag cgctgatcgc gagcactgcg caagcacatg cccaggtcgg
     3301 ctttggcgca accctgtaag cgtatccggc agaatagttt cgaggagcga ttgacggcaa
     3361 cattcgattc gaagagcggt ccgatggtgg tcaagcttga cgagaagtcc gtcgtacgca
//
LOCUS       DASUTG010000911         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2218511, whole genome shotgun sequence.
ACCESSION   DASUTG010000911 DASUTG010000000
VERSION     DASUTG010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218511"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF089_21130"
     CDS             <1..616
                     /locus_tag="VF089_21130"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6176519.1"
                     /translation="EKCSYHGKYVSFDDVGLGWKPIRSNIPIYLPATATIGLRLAGEI
                     ADGVVLNAVCSPEYTVNALKIVKESAEKAGRDFASFQVAQIINCSVEDDHQKALDAVR
                     WEVATKLDPVQISFIAAPKMRVGEPYIRKEDIPLFEKAHADGGMDGLIKAIPDSYVEG
                     MTASGTPDEVQKRVQQYRDAGVQIPLLRPAAAHQTQRLLDLFAQ"
     gene            708..2786
                     /locus_tag="VF089_21135"
     CDS             708..2786
                     /locus_tag="VF089_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX6176520.1"
                     /translation="MYRVGIDIGGTFTDMLLVSEDGAAVIGKTLTTPTDPSLAVENAL
                     KPALQNGSVNPQQRGTLIHGTTLVTNALIERKGAPTALLTTSGFRDAVEIGREHRYEL
                     YDLNLDLPKPLVPRHLRFDVPERIAADGSLLQPLDEEFVRRLVAELRDKGIKAIGISY
                     LNSYRNPAHERRTGEIVAEVAPLIRVSLSSEVVSEIREFQRTSTTLANVYVQERVSGY
                     LAHLEARLQAIGFNGSFFVMLSSGGIATRDTASRFPVRLLESGPAAGALAAAQAGMLS
                     GHRDLLSFDMGGTTAKLCVIEDGRPLKTHEFEVDRVYRFRKGSGLPVRIPVIDMIEIG
                     AGGGGMARIDSLGLLKVGPESSGADPGPVCYGQGGTEPTVTDADLILGYLDARYFLGG
                     KMQLDVDAARDALGRFGRRLGMTVEQVAWGIHQIVNENMANAARAHLGERGKDPRRLP
                     MYAFGGAGPVHGYGVAEILRLPALISPVGAGVGSTFGLLAAPLAFDFVRSAYSRLDEL
                     DWQFANTLLNEMAAEGRQVLESSGLSDEEISYQRTADMRYVGQGHEVSVSLPDGRLGV
                     KHLETIIGTFENTYQALYGRKGPDVPLEIINWRVVARGPQPQLDMKLEREPVGAGNAI
                     KGSRSAYFSARGSYGETPVYDRYGLKPGMTFAGPAIVEERESTLIIGARGRARVDERL
                     QIVVDLTDEG"
     gene            2776..>3870
                     /locus_tag="VF089_21140"
     CDS             2776..>3870
                     /locus_tag="VF089_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX6176521.1"
                     /translation="MKDKTIDPITLEVIWNRFLSVANEQQDALIRTAFSTIVRESQDL
                     ACGLFDTQGRMIAQSLSGTPGHINAMASSMKHFLAAFPADRLSPGDVLITNDPWQTAG
                     QINDITITTPVFKQDRLVALFANTCHSADIGGRILSAEAREVFEEGLRIPIMKLFDRG
                     EPNRILMQIVRTNVRQPDEVIGDFYAQTACNDAGGRALLEMMEEFELDSIDPVADEII
                     RRSETAVRAEIARLPSGEWSNETWSDGFEEPIVVRCKVKVHGDEIFIDFTGSSPQSTR
                     GINVVLNYTHAYASFAIKAAICPDVPHNDGSFRPVHVSAPAGSILNALEPAPVASRQV
                     IGHFIPSAIFAALSGAMPGRLMAPGADPIWL"
BASE COUNT          823 a         1041 c         1162 g          844 t
ORIGIN      
        1 cgagaagtgc tcctaccatg gtaagtatgt gagtttcgat gatgtcggtt tgggctggaa
       61 gccaattcgt tcgaatatcc ctatctatct tcccgccacc gcgacaattg gcctacgcct
      121 ggcgggagag attgccgacg gagttgtcct taacgccgtg tgctcaccgg agtacaccgt
      181 caatgcgttg aaaatcgtca aggaatcggc ggaaaaggcc gggcgggact ttgccagctt
      241 ccaagtcgcg cagatcatca attgctcggt ggaagacgat catcaaaagg ctctcgatgc
      301 agtcagatgg gaagttgcca ccaaactaga tccggtacag atttcattta tcgccgcgcc
      361 caagatgcgg gtgggcgaac cgtatatcag aaaggaagac atcccgcttt tcgagaaggc
      421 tcatgccgat ggcggtatgg acggactgat caaggcgatt ccggattcct acgtggaagg
      481 catgacggcc agcggcacac cggacgaagt gcaaaagcgg gtgcagcagt accgtgatgc
      541 cggcgtgcaa atccctctcc tgagaccggc ggcggcgcac caaacccaac gcttgttgga
      601 tttgtttgcg cagtgaccct tcaattcaag ttcaatccca aagtcaggaa agtcgctcct
      661 gagcattgga taaccaaggc tcgggcaagc agcttctgaa ttgagttatg tatcgggtag
      721 gcatcgacat tggcggcacg ttcaccgaca tgctgttggt gagtgaagac ggtgccgccg
      781 tgatcggcaa gaccttaacc accccgaccg atcccagcct ggcggtggag aatgctctca
      841 agccggcgct gcaaaacggc agtgtaaatc cccagcagcg tggcacctta attcatggca
      901 ccaccttggt aacaaacgcg ctcatcgaac gcaaaggcgc gcccacagcc ttgctaacca
      961 catccgggtt ccgcgatgcc gtcgagatcg gtcgcgagca ccgttacgag ctttacgact
     1021 taaatttgga tttacctaaa ccgctggtgc cgcgccattt gcgcttcgat gtcccggaac
     1081 ggatcgccgc cgatggcagt cttctgcaac cgctcgatga agaattcgtg cgccggctag
     1141 ttgcagagtt gcgcgacaaa ggcatcaaag ccatcggtat cagctatctc aatagctacc
     1201 gtaatcccgc tcacgagcgc cgtaccggcg aaatcgttgc agaggtcgcg ccgttaatcc
     1261 gcgtctcgct cagctcagaa gttgtttctg aaatccgcga atttcagcgt accagcacca
     1321 cactggctaa tgtttatgtc caggaacgag tgtcgggtta tctggcgcat ttagaagctc
     1381 ggctacaggc gatcggtttt aatggaagct tctttgttat gctttcgagc ggtgggattg
     1441 caacccgaga taccgcgtca agatttccgg tgcggctgct tgaatccggg ccagctgctg
     1501 gcgcactggc tgcggctcag gccggcatgc tttccggtca ccgagatttg ttatcgtttg
     1561 acatgggcgg caccaccgcc aagctctgtg tgattgagga tggccggccg ctgaagaccc
     1621 atgaattcga ggtcgaccgg gtttatcgtt tcagaaaagg cagcgggctg cctgtgcgca
     1681 ttccggtgat cgatatgatc gagatcggcg ccggcggcgg cggtatggcg cggatcgatt
     1741 ctctcgggct tttgaaagta ggtccggaaa gttccggcgc agatccaggg ccggtctgtt
     1801 atggacaagg cggcaccgaa ccgacggtca ccgacgccga tctgattctc ggctacctcg
     1861 atgcgcgcta ttttcttggt ggtaagatgc agctggatgt cgatgcggcg cgcgatgctc
     1921 tcggccgttt tgggcggcga ctcggcatga ccgtcgaaca agtcgcctgg ggcattcacc
     1981 aaattgtcaa cgagaatatg gcgaatgccg cgcgcgctca tctgggagaa cggggcaaag
     2041 acccgcgccg gctgccgatg tacgcgttcg gtggggcggg gccggttcat ggctatggtg
     2101 ttgccgaaat cctgcgactg ccggcgctga tttcccccgt cggtgcagga gtgggatcga
     2161 cgttcggctt gctcgctgca ccgttagcat tcgattttgt ccgcagcgcc tacagtcgtt
     2221 tggacgagct agactggcaa ttcgccaaca ctttgctgaa cgagatggct gcagaaggac
     2281 gacaggtact ggaaagctca ggattatcgg acgaagagat ctcctatcaa cgtacggcag
     2341 acatgcgtta cgttgggcag ggacacgaag tttccgtgtc tctgccggat ggaagattgg
     2401 gcgtgaagca tcttgagaca atcattggaa cattcgaaaa tacctatcaa gcgctgtacg
     2461 gtcgcaaagg acctgacgtg ccgctcgaaa ttatcaactg gcgcgtggtt gcccggggtc
     2521 cgcagcccca gctggatatg aagttagagc gcgaacccgt gggagccggc aatgctatca
     2581 agggatcccg ctcggcttac ttttctgcgc gcggcagcta tggtgaaact ccggtttatg
     2641 accgttatgg gttgaagcca ggaatgacgt ttgccggacc cgcaatcgta gaggaacgtg
     2701 aatccacttt gatcatcggc gcgcgcgggc gcgctcgggt ggacgagcgg cttcagatcg
     2761 tcgtggactt aactgatgaa ggataaaacc atcgatccga tcacgctgga ggtgatttgg
     2821 aatcgttttc tttcggtggc caacgagcag caggatgcgc tgattcgtac cgcctttagc
     2881 actattgtgc gagaaagcca ggatctggcg tgtggcttgt tcgacaccca aggacggatg
     2941 atcgcgcagt ctctgagcgg tacaccgggg cacatcaatg ccatggctag ctcgatgaag
     3001 cactttctcg cggcttttcc agcagaccgg ctctccccgg gcgacgtttt gattaccaat
     3061 gatccatggc agaccgcagg tcagatcaac gacattacga ttaccacacc ggtgtttaag
     3121 caggatcgct tggtcgcgtt gtttgcgaac acctgtcata gcgccgatat cggcggccgg
     3181 atactttccg ccgaggcgcg cgaggtcttc gaagaggggc tgagaattcc gatcatgaag
     3241 ttgtttgatc gcggcgagcc taatcggata ctgatgcaga ttgttcggac taatgtgcgc
     3301 cagccggatg aagtcatcgg tgatttctac gcgcagaccg cttgtaacga tgccggcggg
     3361 cgtgcgcttc tcgaaatgat ggaggaattc gagctcgaca gcatcgaccc ggtcgcggac
     3421 gagatcatcc gccgttccga aactgcagtg cgcgccgaaa tcgctagatt gccgagcggt
     3481 gaatggagca acgaaacctg gagtgatggt ttcgaagagc caattgtggt tcgctgtaag
     3541 gtgaaggtgc acggagacga gatcttcatc gacttcactg gctcatcgcc gcagagcacc
     3601 cgcggcatca acgtggtgct caactacact catgcctacg ccagtttcgc catcaaggcg
     3661 gcgatttgtc ccgacgtgcc gcacaacgac ggcagtttcc ggcccgtgca tgttagcgct
     3721 ccggcgggaa gtattctaaa tgcgctggag cccgcgcctg tggcgagccg tcaggtgatc
     3781 ggtcacttta ttcccagcgc gattttcgcc gcactctccg gggctatgcc ggggcgtttg
     3841 atggcaccgg gagccgatcc catatggttg
//
LOCUS       DASUTG010000912         8334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_143418, whole genome shotgun sequence.
ACCESSION   DASUTG010000912 DASUTG010000000
VERSION     DASUTG010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143418"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF089_21145"
     CDS             <1..497
                     /locus_tag="VF089_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6176522.1"
                     /translation="HHNYLSWVVLKAHTRNTAGAVTLRSSDPRDTPEVNFRYFDEGSD
                     TNGEDLDGVVEGVKFVRQMTRALESEKIIAEEELPGSQVRSDAELRDYVRNNAWGHHA
                     SCTCAIGPKERGGVLTSDFKVHGTSGLRVVDASVFPRIPGFFIVSAVYMIGEKAADVI
                     LAES"
     gene            complement(655..3435)
                     /locus_tag="VF089_21150"
     CDS             complement(655..3435)
                     /locus_tag="VF089_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX6176523.1"
                     /translation="MKRTFLLCFLLVLLFLGAYPFLRRTQVGSCLAYALGGLREVAQE
                     RSARFLGKVHEDSARCRGGESALAWRNTPWIDWQRYSAAAGPESRTTGLAGKLGFFSP
                     DSRGINGALLDLEFQRLELLKFNLFDNSGTFETYMRDWHSPSGHVTKTWPELRLPKDH
                     RFYAEVGADGPQLCSGELIRFRTISGICNDITNPLMGSSGQPFARNVDFESTFPELAV
                     DSLAANRHGGRIGLLKPNPQLISQRLFTRPLDDACDDHMLRKDSAEASCDYQKASHLN
                     LLAGFWIQFMTHDWFSHLDEGQNQSAMMAMGCDNHHPSNDERRPSAARTQPIGCRPDD
                     RIDKSLIAQEMPAPTFKLGKNSHMTRAHKTTANKVTAWWDASQIYGYDETSRRRVKRD
                     QRDPAKLLLERANTGHEAAAYLPKLNSADPMNPQWIGQEAVAFPDNWNIGLSFFHNVF
                     AREHNLFVDAFRFEAKSNPDADSGLRRPEHPDQVISNKEVTAGELFEVARLVVAAEIA
                     KIHTIEWTTQLLYNEPVYLAMNANWSGLFNEHPLVNSALERVIHRFTRSANDKKATGW
                     YAAFAAGPGIIGLGNTQADVNAGINHFGSPFNFPEEFINAYRLHPMLPDFIEFRELNG
                     DRNVVKSKIAVSETLRGEATRAMHQHGPANWALSFGRQRLGRLTLRNHPRFLQSLSMP
                     RLNSGSGKIDVAALDLIRDRERGIPRYNEYRRQYGLKQLTKFDDFIDARQAKNLEQQE
                     LVKLLREIYGQHRCDSSKIITRAQKNDDGSQINDCLGHPNGSLVDNIEDIDAMVGWLA
                     EFARPHGFAISETQFQVFILNASRRLFSDRFLTSSFRPAFYTTLGYNWVNNNGPGGKI
                     FEPKKANGHTVEVSPLKRVLQRTIPELREELEQVVNVFDPWARDRGEYYSLDWKPRRG
                     AEADVAFATGVR"
     gene            3671..4549
                     /locus_tag="VF089_21155"
     CDS             3671..4549
                     /locus_tag="VF089_21155"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6176524.1"
                     /translation="MSAISAEFYALANAFLFAFHNMLTKKAMRYSNPATGVISSLLIN
                     IVFLWSVSILFVPLSSLTSASILIFVVVGFFQPGLTRLLTYKGIDALGVAITDPIRAT
                     TPLFSAMMAIVFLGEQITLPIVIATLLIIAGITLLSWRSGSVKLAGSAIFLWYPIGAS
                     ALAGASQVVRKFGLAAVPHPFLAAAVTATSSFIVSLLTLWYVEKSQETWKMNRQCFWW
                     FLAAGITISLGMVCIYYALDLGKVSVVIPISSTGPFFSLIFSALFLRDVERVTLRIVI
                     SAAMIVGGVLLLTLWK"
     gene            4841..5539
                     /locus_tag="VF089_21160"
     CDS             4841..5539
                     /locus_tag="VF089_21160"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX6176525.1"
                     /translation="MINDSCHSNRIINQRVKIDLGFLKLVIVLIAVALPGLALKNDEA
                     VAANAADQPGTYDSAFGYGKQSLGISGGYGLALPVGGTDAPELEDIEFVYVTPRWGVG
                     ITDPLGGSSWYRGNFELLLEGTFLYMLEPKSGIAGGIAPGLRYNFLTGTRVIPFLQAG
                     VGFIALDADLTRQSDGLNFILQTGLGLHYFITQRSALTGEWRFHHISNASIHDKNAGI
                     NSSLFMLGLTFFLR"
     gene            complement(5775..6023)
                     /locus_tag="VF089_21165"
     CDS             complement(5775..6023)
                     /locus_tag="VF089_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176526.1"
                     /translation="MDKHVGMGVFVGGIFAATIASIAAIDGFAAGGFVLLASVVFGLA
                     AGLCIGGLIVANFAMLTVEEKEKEEVVAQSRSTARAAA"
     gene            complement(6697..7830)
                     /locus_tag="VF089_21170"
     CDS             complement(6697..7830)
                     /locus_tag="VF089_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176527.1"
                     /translation="MAKFFLSAVLLSLSVSCATISTEAPDRAQEPSRFDSFESSFTSF
                     ERLAEIQDRAKPIENSFSLNFGPQLLNVKASYLEKAELVPATTVDSPQPNLRRYLKLL
                     GSTSFGGSNLTGEGELTYGSGNTVPGDCDCLDWPRMMRLGIKSRWQGLRYGVDIKSVE
                     RGFVTISGIESAEQRDEGQLWGEYNLGPFNLRGSVVESWEELLDANRHRITRAATTAV
                     QLNRSVWSGTLLSTYGLVEQGPDLNQETTVFSNRLAGLYRPFSVLSLGPSIGIKHEWD
                     TATGYRTESPTTEFAIIYTPVRDKFRLTGGTSLTRTFNRYALTDIMTVGTRAIVDWKI
                     GNFLGRDDTLSFNVNYNRQRDLIATGNSSDHLSGILQLKISGF"
BASE COUNT         1861 a         2177 c         2235 g         2061 t
ORIGIN      
        1 cgcatcacaa ctacttgagc tgggtggtgc tgaaagcgca tacccgcaac acggccgggg
       61 cggtgacctt gcgctcgtca gatcccagag atacccctga agttaatttt cgctatttcg
      121 acgagggcag cgacacgaat ggagaggatc tcgacggcgt tgtcgaaggc gtcaagttcg
      181 tgcgccaaat gaccagagcg ctcgaatcag aaaagatcat cgccgaggag gaattaccgg
      241 gcagtcaggt tcgatcggat gcggaactac gcgactacgt gcgcaacaac gcctggggtc
      301 accacgcctc ctgcacctgc gcaatcggcc ccaaagaaag aggcggggtg ctaacaagcg
      361 atttcaaagt gcacggcacg agcggactgc gggttgtcga tgcttccgtg ttcccgcgca
      421 ttccagggtt ttttatcgtc agcgccgtat acatgatcgg tgaaaaggcc gcggatgtga
      481 tcctggcgga aagctgatga gaaagtccgt ggactccgct tcgcttcgtg ccggattcac
      541 tgccgggaaa atattggccc cggggtaaca ggaggcacca cattgaaaac ttttcgtttc
      601 tgcgccttcg ctcctccagg ctaacccctt gatctgtcta cctactgttc tttactaccg
      661 aactcccgtc gcgaacgcca cgtccgcttc tgcgccgcgc cgcggcttcc agtcgagcga
      721 gtaatattca ccgcgatccc gtgcccacgg gtcgaaaaca ttcaccactt gttcgagttc
      781 ttcacgcaat tctggaatgg ttctttgaag cacgcgcttt aatggcgaga cttcgactgt
      841 gtgtccgttg gctttcttcg gttcgaaaat cttaccgcca ggaccgttat tgtttaccca
      901 gttgtagccc aaagtcgtat aaaacgccgg tcgaaaacta gaggtcaaaa agcgatcgct
      961 gaaaagccgc cgggatgcgt tcagaatgaa aacctgaaat tgtgtttccg agatggcaaa
     1021 accgtgcggc cgcgcgaatt cggcgagcca tcctaccata gcgtcgatgt cctctatgtt
     1081 atctaccaag ctgccgtttg gatggccaag gcagtcgttg atttggctgc catcgtcatt
     1141 tttctgcgcc cttgtaataa tcttcgaaga atcgcagcgg tgttgtccat agatttcacg
     1201 gagtagcttc accagctctt gctgctccaa gttttttgcc tggcgggcgt cgatgaagtc
     1261 atcaaacttg gtcagctgtt taagaccgta ctggcgtcga tattcattat aacgcggtat
     1321 gccgcgttcc cggtcacgga tcagatccag cgcggcaaca tctattttcc cgctgccgct
     1381 atttaggcga ggcatagaga gactttgcaa aaatctggga tggttgcgca gtgtcagtct
     1441 gccgagtcgc tgccggccga acgagagcgc ccagttggcc ggcccgtgct gatgcatcgc
     1501 gcgagttgct tcgccgcgca gggtttcgga tacagcaatc ttcgatttga cgacattacg
     1561 atcgccgttt agctcgcgaa actcgataaa gtcgggaagc attggatgca agcggtaggc
     1621 attgatgaac tcctcgggga agttgaacgg ggaaccgaag tggttgatcc cggcattgac
     1681 gtcggcttgc gtattgccga gaccgataat accgggcccc gcggcgaaag cggcatacca
     1741 tccagtggcc ttcttgtcat tggccgatcg agtgaaccgg tgaatcaccc gttcgagtgc
     1801 gctgttgacc aaagggtgct cgttgaatag acccgaccaa ttggcattca tagcgaggta
     1861 gaccggctcg ttgtaaagca gctgggtggt ccactcgatc gtatggatct tggcgatctc
     1921 ggcggccacc accagacgcg cgacctcgaa gagctcgccg gccgtgacct ccttgttgct
     1981 gatcacttga tccgggtgtt ccggtcgccg cagtcccgag tccgcatccg gattgctttt
     2041 cgcttcaaac cgaaaggcat cgacgaaaag attatgctcg cgcgcgaata cattgtggaa
     2101 aaaacttaga ccgatattcc agttatccgg gaaggcaaca gcttcttggc cgatccactg
     2161 cggattcatg ggatcggcag agttcaactt cggtagataa gccgcagctt catgccccgt
     2221 attagcgcgc tcgagcagta gcttggccgg atcgcgctga tcgcgcttca cccgccggcg
     2281 cgaagtctcg tcatatccat aaatttgcga agcatcccac cacgcggtga ctttgttggc
     2341 ggtggtttta tgcgcacgcg tcatgtggga gttcttgccg agcttgaagg tcggcgccgg
     2401 catttcctgc gcaatcaaac ttttatcgat gcgatcgtcc gggcggcagc caatcggttg
     2461 ggtgcgcgcc gcgctcggtc tacgttcatc gtttgacggg tgatgattat cgcagcccat
     2521 cgccatcatt gccgactggt tttgtccctc gtctagatgc gaaaaccaat catgggtcat
     2581 gaactgaatc cagaatccag cgagcaaatt tagatgcgac gctttttggt agtcgcaact
     2641 cgcttctgca gaatctttcc gtagcatgtg atcatcgcat gcgtcatcaa gcggccgggt
     2701 gaatagcctt tggctaatca gctgcggatt gggctttaac aagccgattc gaccgccgtg
     2761 gcgatttgcc gcaagcgagt cgacggccaa ctcgggaaag gtgctctcga agtcaacgtt
     2821 gcgggcaaac ggttggccgc tggagcccat cagcggattg gtgatgtcgt tgcagattcc
     2881 gctgatcgtg cgaaagcgaa tcagttcgcc ggaacacagt tgagggccgt cggctccaac
     2941 ctctgcgtag aaccggtgat ccttagggag ccgtaattcc ggccaggtct ttgtgacatg
     3001 acccgatggg ctgtgccaat cgcgcatgta ggtctcgaag gtgccgctgt tgtcgaacag
     3061 gttaaacttg agcaactcga gacgctgaaa ctcgagatcg agcagcgcac cgttgatacc
     3121 gcggctgtcc gggctgaaaa aaccgagttt gccggccaaa ccggtggtgc ggctctcagg
     3181 ccccgccgca gccgagtaac gttgccagtc gatccacggt gtgttgcgcc aagccagcgc
     3241 tgattcaccg ccgcggcaac gcgcgctgtc ttcatgaacc tttccaagaa agcgcgccga
     3301 ccgttcctgg gcgacttcgc gcaagccgcc aagcgcatag gcaaggcagc taccgacctg
     3361 agttcgtcgg agaaaaggat aggcgccgag gaagagtaag actagcagga aacaaagcaa
     3421 gaaagttctt ttcatgggcg ccgattagat cctacgaacc agtcgatgag ctctactcta
     3481 agctttccag tatgcagcaa ggggcttcaa agagtcaatt ttctcacagg gccgagcatc
     3541 tcatgatagc gcttgaatca ctgttatttg ccctctgatg ctaaaaactg tatagctcag
     3601 gggcacgtcc aaccaccaga taaccaacgt ctaagatccg gcaaaattct tccgacatac
     3661 tccactcaat atgtctgcca tttccgctga attctacgcc ctggccaacg cttttctctt
     3721 cgcattccat aatatgctga ccaagaaggc gatgcgctac tccaatcctg ccaccggggt
     3781 gatcagtagc ctgttgatca acatcgtatt tctctggagc gtgtcgatcc tgttcgtgcc
     3841 gctgtcgtct ttgactagcg catcgatttt gatttttgtc gttgtcggat tttttcaacc
     3901 cggattgacc cgcctgctca cctacaaagg gattgatgcg ctcggcgtcg cgattacgga
     3961 tccaatccgc gccaccacgc cgctgttcag cgcgatgatg gcgattgtct ttcttggcga
     4021 gcaaatcacc ttgccgattg ttatcgccac tttgctgatt atcgccggca tcaccttgtt
     4081 gtcctggcgc agcggttcgg tgaaactcgc gggctccgcc atctttctct ggtatcccat
     4141 cggcgcctcc gcgctggcgg gagcctctca ggtcgtgcgc aaatttggtt tggcagccgt
     4201 tccccatcca tttctagcgg cggcggtgac cgcgacttcc tctttcatcg tttcgctttt
     4261 gacgctctgg tatgtggaga agagccaaga aacctggaaa atgaaccggc agtgtttctg
     4321 gtggtttctc gccgccggca ttaccattag tctgggcatg gtgtgcatct actatgcgct
     4381 cgatctgggt aaagtctccg tcgtgattcc aatcagcagt accggaccgt ttttttcgct
     4441 gatctttagc gcgctctttt tacgcgacgt ggagcgtgtc acccttcgaa ttgtcatcag
     4501 tgccgcgatg atcgtcggcg gcgtgttgct cctgacctta tggaagtgat ccttgttttt
     4561 aagagtgagt taatctctgc agaagaactg cagccgacgg tctcgttcaa cgccagtcaa
     4621 gatgctattt accggagata tttacgtttc ctagcgggca gaaagcaggc tcgcaagctt
     4681 tacttgggga cttgtagtag agccacttgc ttgcatagat gaattccatg agcatatatg
     4741 cgccatggca gagcggaggc tggaaatttg gaatgatctc tgaaagatct atgaagtcag
     4801 atatctagta acgcggggcg atatcaggag gttccctcaa atgatcaacg actcgtgcca
     4861 ttcaaaccgc atcatcaatc aacgggtaaa gattgattta ggcttcctta agctggttat
     4921 tgttttgatt gctgttgctc tgccgggatt ggcgctaaag aatgatgagg ctgtcgcggc
     4981 gaatgcggca gaccagcccg gcacatatga ctcggcattt ggctacggca agcaatcgtt
     5041 aggaattagc ggaggatatg ggttagcact cccggttggt gggactgacg ctcctgagct
     5101 ggaggatatt gagttcgtct acgtgactcc gcgctggggc gttggcatca ccgatccctt
     5161 gggagggagt agttggtacc ggggcaactt cgaactgtta ctcgaaggga cctttctcta
     5221 catgctcgag cccaaaagtg gcatcgccgg aggtatcgca ccggggctac ggtataattt
     5281 tttgaccgga actcgtgtga ttccttttct acaagctgga gtaggcttta tcgctctgga
     5341 tgcggatctg accaggcagt ccgatggttt gaatttcatc ctgcaaacag gtttagggct
     5401 tcactatttc attacccaac gtagcgcgtt gaccggagag tggcggttcc accacatatc
     5461 gaatgcgagt attcacgaca agaatgccgg tattaacagc agtctgttta tgctaggcct
     5521 gactttcttt ctcaggtaaa gttcactgct cgcctaattc tttgattgtt tgctgcagat
     5581 aactacgatc gatgtatttt attggatccg gcggtgcgat ctttgactgt tcggcaagga
     5641 tctcaagggc gaatttcatt ccctccagat tcagattgaa tatccgcacc agccgtaagc
     5701 ctcaaatttt gtcagccggc acggcatgca gataaaaatg aacccttgcc ggccggctag
     5761 ttcggtggac gtggttacgc cgcggcccgt gcggtgcttc ggctctgcgc aacgacttct
     5821 tccttttctt tctcttccac tgtgagcatg gcaaaattcg caacgatcaa gccgccgata
     5881 cacaggcctg cggccaaccc gaagactacg gaggccagta gtacgaaacc accggcagcg
     5941 aagccgtcta ttgccgctat cgaagcgatg gtagccgcga agataccgcc gacgaagacg
     6001 cccatgccaa catgtttgtc catagtaaag tctccttcag gtgtatgatg aggatacagt
     6061 tccgcaaaga acgcgccaat tgattccgcg agttacggca cgattcagcg gctccgtgaa
     6121 cgcttggttt cttagttccg gcaaattgca gctgctcgat tcccgaaatt aagaaatcag
     6181 ttttaggttt gctgccaatg ccacttggac tgttgactga ggctcgccaa gtagacgttt
     6241 gtctctacgt gatagcttcg gtttcgcgcg aaggctctga caacacatct tactttattc
     6301 cttaatgtga ttgtttcaat gtggcgcgaa ccggcgggtc cgctgtgacg ggcgttctga
     6361 tcgcagtttt tggcaccggg aggtcgtgat acgtttatct tcgcggcatc gactggggtc
     6421 gtgccggtgt cggggcgatg ctcataggcg cgctgtcaac ctctaatcgg ggctcgctgg
     6481 acggctgctt cgatgagtat gctggagact acaacgatgg tcgtattcta tctctcaatg
     6541 actcatgccc gggagataaa ctagagttca tccccctatt cgcaaccgaa caaagagcgt
     6601 gcgcacaggc accgtgctgt ctgccaaagc gcgctcggca gataagatat ttctgaaagc
     6661 gctataccta tactgcagat ccgatagcat ggcgctttaa aatccgctaa tttttaactg
     6721 taggatgccg gagagatgat cagaggagtt acctgtcgca atgagatcgc gctggcggtt
     6781 gtaattgaca ttaaaggaaa gcgtgtcgtc gcggccgaga aagttgccaa ttttccagtc
     6841 cacaatggct cgggtgccaa cggtcataat gtcagttaag gcatatcgat tgaaggttct
     6901 ggttaatgag gtgccgcctg tcaacctgaa tttatcccgg actggcgtgt aaataatagc
     6961 aaattcggtc gtcggggatt cggtccgata tccggtggct gtatcccact catgttttat
     7021 accgatactt ggacctagag ataacacact aaagggacgg tataaccccg caagtctatt
     7081 gctgaagact gtcgtctcct gattcaaatc cgggccttgt tccaccagtc cataggttga
     7141 taatagggtt ccgctccaca ctgatcggtt caattgcacg gcggttgttg ccgccctagt
     7201 gatgcgatgg cgatttgcat ccagaagctc ttcccacgat tcaacgaccg aaccacgcag
     7261 attgaacgga cccagattgt attctcccca aagctggcct tcgtcgcgtt gctcggcgct
     7321 ttcgattccc gaaatcgtaa caaatcctcg ttctactgat tttatatcta ctccataacg
     7381 taacccttgc caccgagatt ttattcccaa ccgcatcatc cgcggccaat ctagacagtc
     7441 acaatcacca gggacagtat ttcctgagcc gtaggtcagc tcgccctcgc cggtcagatt
     7501 gctgccgcca aacgaagtcg atcccagtaa cttgagatac cgccgcaggt taggctgagg
     7561 tgaatcgaca gttgttgccg ggaccaattc agctttttca agataggacg cctttacatt
     7621 taggagttgt gggccgaagt ttaacgagaa gctattctca atgggtttag cgcgatcttg
     7681 aatttccgcc aagcgctcaa aactagtaaa gctcgactcg aaactgtcga atctgctggg
     7741 ttcttgggca cgatctggag cttcggtgct gatcgttgcg cacgaaaccg ataacgacag
     7801 aagaactgcg gaaagaaaaa atttagccac tgcgttactc gttgcaaagt ctagcacatt
     7861 gagttccgtg aacaaaaata ctgtttcagg tatttcgatc gccgaattct tacatcaaga
     7921 atttctcggg ttctgggagt tctgaactaa ggactttaag ctcaaccgat ctaattaggc
     7981 cgctacaggt aaattttgca gacaaaaaaa cacacctgtc gtggctaaga acgcatgcga
     8041 cttgggatca aggcctcgac tggtgaatag atcatgggat cagaatgctt tgccaaatct
     8101 ccagtaacct gttcactggc tgaaaggtgg gttgtaagat catctttgcc agttcggctg
     8161 atgagtaccg agaaagcgcc ggtgtgtgcg aggaacctga gcgacagagc ggtgctggag
     8221 aagcaagtag gaaagcttaa gaaggcttcg aacgacgcag atcttgattc gatgatcctg
     8281 ctgcgctttt cactggaagg acaactcgta accctgaacg gctttatcgc gagg
//
LOCUS       DASUTG010000913         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1922900, whole genome shotgun sequence.
ACCESSION   DASUTG010000913 DASUTG010000000
VERSION     DASUTG010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922900"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF089_21175"
     CDS             complement(<1..1056)
                     /locus_tag="VF089_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6176528.1"
                     /translation="MNSKPIDLTEVSSSTSRLSNVLTNKRLKLWLWLGLSLLLLAACI
                     VIEFKTSIVQAWIFTRTNERLSFELAPGAASSIAFPAPAPFDDRRGYSKLADFQSRLL
                     SHGFQVSQQVRQSETMVNLISQGISPPYTEPPETGIDIRGAAGVPLFRHEQSEFLFEK
                     IDDIPPVLVKTLLFLEDRDLDNPNFSRQNPAVEWDRFFKAALLYLGSKLYLDVPVQGG
                     STLAVQLEKFRHSAHGRTDSPLDKFRQIVGASLKAYRAGPNTRAWRDRIIVDYFNTVP
                     LAAAPGYGEIHGLGEGLYAWFGMQLADAIRALRTPGITIPKVRVYKHVLTLLISVRAP
                     SVFLVEERESLEHKVNQF"
     gene            complement(1341..1766)
                     /locus_tag="VF089_21180"
     CDS             complement(1341..1766)
                     /locus_tag="VF089_21180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6176529.1"
                     /translation="MEVKDVLALAKERKKGQRRLDLFRRGNLETWILYFPPGDVQRMH
                     NHPSDRTYYVLSGRGVMKGLKETHELSPGQIISIPAHEYYEGSNPHEEPMILLGNSHK
                     ATPEDLARAGGQRNEIDDKTGKRIIYQHGGGRDEGVLAD"
     gene            1933..2832
                     /locus_tag="VF089_21185"
     CDS             1933..2832
                     /locus_tag="VF089_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176530.1"
                     /translation="MELAKINVMYGHLEGTEGKFARDPSGYLSIDAGIYQKYGLEVSW
                     RHVQGTEARYQQLESGGAHISMVVGRASLQHFLDTKKTRLLGCVMNSCTYYLMTAQGV
                     RAIGELKNKAISCREGPAQGVPFGRLLEERAGLKLGKELTLQFTESDQDAFRLLLGGQ
                     VQAAWLPRPYAFIAEEKGFARLNEGTDIVDDPLPITMETTASLSRERAGDFSRFVQAH
                     REGIRYLRNHKDETLSLLQTRFGHSPELAAKTFDEYLVCMDDRLIVDFQHLRKLVAQI
                     APNIPGGARQIASEWIISGALRI"
     gene            3039..>3801
                     /locus_tag="VF089_21190"
     CDS             3039..>3801
                     /locus_tag="VF089_21190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176531.1"
                     /translation="MPGIIDADTHISESEAMWSLLDKEMYHRRPVLLKAPEDTLYGPR
                     NAFWLIDGSIFPKPNGKGSFRLITPSASKLESSRGDIHLAYREMTDIPGRLKDMDKLG
                     VEKQVVFPTLFLIYITDDTALDVALARAYNRFLAQACAKSGGRMSWVVVPPLHSVEES
                     VKEIKWGKDNGAVGVFFRGIEGYRTLDNPYFNPIYRAASDTDLPVLIHTGAGCPLFLN
                     LFDVERNHTFGHSRVQPLFAFRDLIANKIPEQFPRL"
BASE COUNT          901 a          998 c         1044 g          858 t
ORIGIN      
        1 aaattggttc actttgtgct cgagcgattc ccgttcctca accaggaata ctgacggcgc
       61 ccgtaccgag atgagcaacg tcaaaacatg cttgtagacg cgcaccttgg gaatagtgat
      121 cccgggggtt cggagcgcgc ggatagcatc tgcgagctgc atgccaaacc aggcataaag
      181 cccctctccc aaaccgtgga tctcgccgta accgggtgcc gcggcgagcg gaacagtatt
      241 gaaataatca acaataattc ggtctctcca ggcgcgggtg ttggggccgg cgcggtaagc
      301 cttcaggctg gcgccgacga tttgccggaa tttatccagc ggcgaatccg tgcggccgtg
      361 cgccgaatga cggaacttct ccaactgcac cgctaacgtg ctgccacctt ggactggaac
      421 atcgaggtag agtttcgaac ccaaatagag caaagccgct ttgaagaatc gatcccattc
      481 gaccgccggg ttttggcgcg agaaatttgg attatctaga tcacgatcct cgagaaaaag
      541 cagcgtttta accagtaccg gcgggatatc atcgattttc tcgaacagga attcggattg
      601 ctcatggcga aagagcggta cgccggcggc gccgcgaatg tcgatgccgg tctccggcgg
      661 ttcggtataa ggcggagaaa tgccctggct gatcagattg accatggtct ctgattgtct
      721 tacctgttgg ctcacctgga aaccgtggga tagaagccgg ctttgaaaat cggccaactt
      781 cgagtaccca cgacgatcgt caaacggtgc cggggcagga aacgcgatac ttgaggctgc
      841 gcctggcgcc agctcaaacg ataaccgttc gttggtgcgg gtaaaaatcc acgcctgaac
      901 gattgatgtc ttgaactcaa tgactatgca ggcagccagc aaaagcagtg aaagcccgag
      961 ccacaaccat agcttcagcc gtttgttggt taatacattc gagagccggg aagtagacga
     1021 cgaaacctcg gtcaagtcga tgggtttgct gttcattggg gtgagggaag aatgatacca
     1081 taatcggtac aaaatcctaa ctcgaagttg cgtcaggtga gcatccgctc aacgcccgga
     1141 gtagtctatc gccggtgttt caagatgctg ccgtaatctc ggagtcagat gtaatcctat
     1201 ccgcagcggt tactaaataa aagttgtaac tcgcatgcag atacgtatct aataatccgc
     1261 tgaacaagcc gctcaactac aaagtccggt gggaaagcgc ccgaccggac ttttttgcac
     1321 gcgcaaatct tctaatcgcc tcaatcggcg agcactccct catctctgcc accgccgtgc
     1381 tgataaatga ttcgcttgcc ggtcttatca tcgatttcgt tgcgctggcc accggccctg
     1441 gcgagatctt caggggttgc tttgtgactg ttgcccaaga gaatcatcgg ctcctcgtgg
     1501 gggttagagc cttcatagta ttcgtgggcg gggatgctga tgatctggcc cggagaaagc
     1561 tcgtgggttt ctttgagtcc cttcatcacg ccgcggccgc tgaggacgta ataggtccga
     1621 tctgatgggt gattgtgcat cctctgcaca tcccccggag ggaaataaag aatccatgtt
     1681 tccaaattgc ccctgcgaaa aaggtccaag cgccgctgtc ccttttttct ttcctttgcc
     1741 aacgcgagaa cgtccttgac ttccataatc gcctcccttt agtaaagata gttttgcccg
     1801 cacttgcttt gacccgtgac tctattcccg caccaaacgc gctgtcaata tggaacttga
     1861 ccagcaactt atttgtctgc cgcagtcatc cgtaatatag aaaggtgaag ctgaccagca
     1921 caggagagct cgatggaact cgcaaagatc aatgtcatgt atggccacct tgaaggtact
     1981 gaggggaagt ttgcgcgcga cccttccggc tacctgtcca tcgatgcagg aatctatcaa
     2041 aaatatggac tggaagtttc atggcgtcat gtgcaaggca cggaggcgcg gtatcagcag
     2101 ctggaaagcg ggggcgccca tatctccatg gtcgtcggtc gagcatcgct acagcacttt
     2161 ctcgacacaa agaagacccg ccttttgggt tgtgtcatga atagctgcac ctattacctg
     2221 atgacagctc agggggtcag ggcgattggc gaactgaaaa acaaagcaat cagttgccgg
     2281 gaaggtcctg cgcaaggcgt gccgtttgga cgattgttgg aagaaagagc gggcctaaaa
     2341 ctcggtaagg agctaactct tcaattcacc gaaagtgatc aagacgcttt ccggcttctg
     2401 ttgggaggtc aagttcaagc cgcctggttg ccgcgcccat acgcgttcat tgcagaggaa
     2461 aagggattcg caaggctcaa cgaaggcacc gatatcgttg atgacccgct gccgataacg
     2521 atggaaacga cggcttctct atcacgagaa cgcgccggcg acttctctcg attcgtccaa
     2581 gcgcatcgcg agggcatccg gtatttacgc aatcataaag acgagactct aagcctttta
     2641 caaacccgct tcgggcattc gccggagttg gcggcgaaga cgttcgacga atatttagtt
     2701 tgcatggatg accggctgat cgtggacttc cagcatctac ggaaactcgt tgcgcagatt
     2761 gcgccgaaca ttccaggtgg cgcgcggcag atcgcttcgg agtggattat ctcgggagcg
     2821 ctacggatat gataaaaagt tgtttaacag ttcaaccggc gcacaagatg cgcctattca
     2881 aacggttaaa cgacagaaaa gtcgagcgct gtatgacgtt gaaccgttga acgtttgaat
     2941 agcggcgcag gcgcggttga acagattaac ttggccgtac cggttgaaca aaagttctta
     3001 ccagctacat attaaacagt tacagaagga ggccactcat gccagggatc atcgatgccg
     3061 acactcatat ctcagagtcc gaagcgatgt ggagtttgct cgacaaggag atgtaccatc
     3121 gccgtccggt gctgctcaaa gctccggaag ataccttgta cgggcctcgt aatgctttct
     3181 ggctgatcga cggcagtatc tttcccaagc ccaatggcaa gggcagtttc cgtttgatta
     3241 ccccgtccgc gtcgaagttg gaatcctccc gcggcgatat tcatttggct taccgggaga
     3301 tgaccgacat cccgggcagg ctcaaagaca tggacaagct cggggttgaa aagcaggtag
     3361 tatttccgac cttgtttctg atctacatca ccgatgacac agccttggac gtggcactgg
     3421 cgcgcgctta caaccggttc ctggcccagg catgcgccaa atccggaggc cgcatgagct
     3481 gggtcgtggt gccgccgctg cactcagtag aagagtcggt gaaggaaatc aaatggggca
     3541 aggacaatgg cgctgtggga gttttctttc gcggtatcga agggtacagg acgctcgaca
     3601 atccttactt caacccgatc tatcgggcag cgagcgatac ggatctgccg gttttgatcc
     3661 acaccggcgc tggctgcccg ctgtttttga acctcttcga tgtcgagcgc aatcacacct
     3721 tcggtcacag ccgtgtgcag ccgctgttcg cgtttcgtga cctgattgcc aacaagattc
     3781 cggagcagtt cccgcgcctg a
//
LOCUS       DASUTG010000914         7111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2218760, whole genome shotgun sequence.
ACCESSION   DASUTG010000914 DASUTG010000000
VERSION     DASUTG010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218760"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF089_21195"
     CDS             <1..393
                     /locus_tag="VF089_21195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011849779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6176532.1"
                     /translation="GDEMPKKEIVQVNLAAPNPNLSPATRFGNLVFVAGQTGRHPITG
                     EVGTDVREQTRYAVERIKMILETAGTSLDNVLNVLTHLTKREDLAAYNEEYAKYFPTN
                     KPARTTVEAMLNFPELLVEITVTACIPD"
     gene            813..1244
                     /locus_tag="VF089_21200"
     CDS             813..1244
                     /locus_tag="VF089_21200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176533.1"
                     /translation="MAKIKHIAVATQDPEKTAQFYKDVFGLREIARINSPGATGFHLT
                     DGDINFAVLKFKNDQTAGVPQGKAYAGLHHIGFEVDSIEEADKKLTIAGALIRDDIND
                     ALGLGKGQPSHVNAEVKYSAPDGVIIDISETGWAGTAKHPR"
     gene            complement(1267..2193)
                     /locus_tag="VF089_21205"
     CDS             complement(1267..2193)
                     /locus_tag="VF089_21205"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX6176534.1"
                     /translation="MADKGFCGVFPYLVSPIDEQGRVKDAVLARLVNDLIAAGVHGLT
                     PLGSTGEFAYLNREQRHRIVEVVVQATRRRVPVVAGIAATTIADAVSQVNDYDALGVD
                     GILAILEAYFPVPEDGVVAYFKAVANSTALPVVLYTNPQFQRSDLTLSAIERLAQVKN
                     IRYIKDASSDTGRLLTIMNAVGERIRVFSASAHIPACVMLIGGVGWMAGPACVVPRQS
                     VRLYDLCRARQWDEAIRLQRKLWHINRVFAKYNLAACIKGGLEIQGYEVGPPLPPQAS
                     LSDAARAEIKHTLIQLKNIARPTVKKRHSRSA"
     gene            3050..4201
                     /locus_tag="VF089_21210"
     CDS             3050..4201
                     /locus_tag="VF089_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176535.1"
                     /translation="MKERIIIAASVAALIFFVSNAFAQERARQRASEHATVPASQVVD
                     GDYFTFGDTVEISGTINGDLYASGGQVLIDGRINGDVLVAGGRVSLSGTVSQDVRAAG
                     GQVAITGNVGRNLTVAGGNVEVASSAVVRGSVVGAGGSIDLAAPVGGAAKIAAGTLII
                     ANRIAGDVEAAVGTLRITSRADIGGNVSYWSGEDAAVIEGARINGKIVRNVPPQRPRI
                     FPAVFLGWLVFVGINFVCTLVLGLLSARFLPRYHQAVITTLREKPWASLGIGFIAAVV
                     VPVVCALLFATVLAVPLALILLFAFFILLYWSRVYAIGGIGESILTRFRPGSSRASAF
                     VLGLFVYYLLAIIPVVGWLVVPLVMLFGLGSELIARKQFYVTARSQDLI"
     gene            4724..5509
                     /locus_tag="VF089_21215"
     CDS             4724..5509
                     /locus_tag="VF089_21215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176536.1"
                     /translation="MQRIILPTGITVSYAKYGEGPPLVLVHGAFSDYETNWQFVKHLF
                     EKQFTVHAIARRGRGETDATKGHSLDDEGRDVVALVQSIGEPVFLLGHSYGAHTALAA
                     TAQLPNSVRKLVLYEPAWARVVSKDALAQLEKLAQAGEWQEFAMSFFRDRLFVRVNEL
                     DELRTTDLWPPMIADAQASLGDLRALSRYEFNAEAFRGLRMPVMLQIGTESPRELYVT
                     DALAAVLADVRIEELPGQAHEAMTTAPRMYAEAVSRFLLGEAR"
     gene            5929..6558
                     /locus_tag="VF089_21220"
     CDS             5929..6558
                     /locus_tag="VF089_21220"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX6176537.1"
                     /translation="MNKLQEAHVPYNSGWAGELKTGQVIRIIATTTVDFVCMRRENLK
                     ERFDQARTKVYNMKIFLTTGDKLMGRNNQHMMTIVDNTNREGTHDLQKGMCSGYRLQL
                     AKNEGKLRDYYHRDYKDEEIPDHGCYENLSRALAPYGIDPEDVPNPFNLNQHMKIDGA
                     TGRMDHTTVRPKAGSYVDLKAEMDLLVAFSACPDMPVGGKPVDVVIYEP"
     gene            6731..>7111
                     /locus_tag="VF089_21225"
     CDS             6731..>7111
                     /locus_tag="VF089_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176538.1"
                     /translation="MKHRYSAEIQRSRGLLELAAALYDTSLSFQTERAAEQAMQTESR
                     VGYSLTLALTATREKGVSLSFAADGFRELLAVSQGKLSLGWINPSVAATMAFRGKGLF
                     SKRQPIRTIAVFPSYDVMGFAVHES"
BASE COUNT         1621 a         1930 c         1956 g         1604 t
ORIGIN      
        1 ggagacgaga tgccgaagaa agaaattgtt caggtgaatc ttgcggcgcc caatccaaat
       61 ctctcaccag ctacgcgttt tggcaacctg gtgttcgtgg ccggccaaac cggccggcac
      121 ccaataacgg gagaggtcgg cacagacgtt cgcgagcaga cccgttacgc cgtcgaacgc
      181 atcaagatga ttttggaaac cgcgggaacg tctcttgata acgtgctgaa cgtattgacg
      241 catttgacca aacgtgagga cctcgcggcc tacaacgagg agtatgcgaa atatttcccg
      301 accaacaaac cagccaggac cacggtggaa gcgatgttaa actttcccga gctacttgta
      361 gaaataaccg tcacagcttg cattcccgat tgatgaaata gccagagaca cgaaactgag
      421 caaaaggtag atcgttcaac ccttccactc agttcagccc gttgaaccgc tttgctcgct
      481 ttaaattgtt aatcggataa accttcgacc aatcggcccc ggcgctgtcg catgtcgatg
      541 ccgaatgaat caaactcttt tctcgatagc cctggcccac aaacggctga ccccggagtg
      601 tccgctgcct tagggatatg gcttaattca gcacgaacaa ggatcattat tctcctgcgt
      661 ccagtcgttt tctatgcaaa gctttttcag tgtcgttgat gcgttcatca aaggtccctc
      721 gtgaggtaat aagaggggga gcaccgcttg ctctccttga agaactccgc tgtaaagtat
      781 ttaattgcgc aggcgtaatc gagggaggct ctatggcaaa gatcaaacat attgcggttg
      841 ctacccagga tccggagaaa acggcgcaat tctacaagga tgtgtttggc ctgcgggaga
      901 tagccagaat caatagtcct ggcgccaccg gatttcatct taccgacggc gacatcaact
      961 tcgctgtgct gaagttcaaa aacgatcaga ctgccggcgt accgcaaggc aaagcctacg
     1021 ccgggctgca tcatattgga tttgaagtgg atagcatcga ggaagctgac aagaaattaa
     1081 cgattgccgg cgcgctgatc cgagacgaca tcaacgatgc gctgggcctc ggcaaaggac
     1141 aaccgtccca tgtcaacgcc gaggtaaaat acagtgcccc cgatggcgtc atcatcgata
     1201 tttccgaaac cggctgggcc ggcacagcga aacatccacg ctaatctccc tattaccgcg
     1261 aaggattcac gcggagcggc tgtgacgctt tttgacagta gggcgcgcaa tgtttttcag
     1321 ctggatgaga gtgtgtttta tctcggcgcg cgccgcatcg gacagcgacg cctgaggagg
     1381 caaaggcgga ccaacctcat agccctgaat ctccagtccg cctttgatgc atgcggccag
     1441 gttgtactta gcgaacaccc ggtttatatg ccagagcttc cgttgtaatc gaatcgcttc
     1501 atcccattgt cgagcccgac aaagatcgta caatcgaacg ctctggcgag gcacgacgca
     1561 cgccggtccg gccatccagc ctacgccgcc aatcaacatg acacatgccg gtatgtgagc
     1621 ggacgcgcta aagacgcgaa tgcgctcacc tacggcattc ataatcgtca gtagtcgtcc
     1681 ggtatcagaa gatgcgtctt tgatatagcg aatatttttc acctgcgcga gtcgctcgat
     1741 cgcagataaa gtgagatcgc tcctttggaa ctgcgggttt gtataaagga ccaccggtaa
     1801 tgctgtgctg tttgccaccg ctttgaaata ggcgacgacc ccatcctcag gaacgggaaa
     1861 ataagcttcg agaatggcaa ggatgccgtc gactccaagg gcatcgtaat cattaacctg
     1921 actgacggcg tctgcgatcg tcgtcgcagc gatcccggcg acaaccggta cccggcggcg
     1981 cgttgcctgc accaccacct ccacgatgcg atggcgttgt tctcggttca gataggcaaa
     2041 ctctccggtg gatccaaggg gcgtcaggcc atgaacaccg gcagcgatca ggtcgttgac
     2101 aaggcgcgct agcacggcgt ccttgacgcg accctgctcg tcgatgggtg aaaccaaata
     2161 cgggaagacg ccgcaaaatc ctttgtctgc cattcgcggg attctatcca cgtggtcagc
     2221 gcttggcaac tacgttcact gcggttactg tggcggctcg gaccggcgca aacgattgat
     2281 gtgatgctct aaaatgacat aaaacgcatc tatcaacagc taagatctta gaggggagtt
     2341 ttcgatcaaa accatctaag aaaagacgat gccgcggcca accgtcaagt tcagccctac
     2401 tatttctgca catcgccagt tccaattgtt cgatacactc aaccgctgcg ctcacctcaa
     2461 ataattgagc aaagaagagt attcaggaaa acacatgccg ccgatatctt cattgggtat
     2521 tcgacgcgga ggcggtgaga ttgacagtga tcggtgagcc tctaaagcta caagacatta
     2581 ttctgtggaa cgaaataggt tgtctgccag gaaattgagc tcgaagcatg acgcaaggcg
     2641 ggctgtgcac aaagtgaagg acaatgccgt ctccatcatc aacgaagttc tctagtagac
     2701 cacgtaaaaa attacccaag ccgttccaaa gcttcccagc cgctttctct cgcttctttt
     2761 cattgatcag ttctggtttt gtccagatcg gagatggcgc ccgcgacggg cggaccagat
     2821 cgtcagctca tgcttgccct gagtctttca actcttcgcg agccggagat tccgcgattc
     2881 cgcaagccgg ctctctttga actcggcgaa acctttcggt cgtggggtgg tccggattta
     2941 gccagtagta tttcggccaa aatggtgact gaagaattta atttcgacca gggtgacgtc
     3001 tttctgcacg catcgctgtt tgttagccta acaacagaaa ggagattggt tgaaggagcg
     3061 aatcatcata gccgcgagtg tcgccgcgtt aattttcttc gtctcgaatg cgttcgcgca
     3121 ggagcgcgcc cgccagagag catcggagca cgcaaccgtg cctgcctcgc aggtcgtcga
     3181 cggggactac tttaccttcg gcgacaccgt ggagatctcc ggcaccataa acggcgatct
     3241 ttatgcgtcc ggcggtcaag tccttatcga cggccggatc aacggtgatg ttctggtggc
     3301 cggcggcaga gtcagtttat ctggaaccgt ttctcaggac gtgcgggctg ccggtggcca
     3361 ggtggcgatt acaggaaacg tcggcagaaa tttgaccgtg gccggaggca acgtggaggt
     3421 cgcctcatcg gcagtagtcc gcggtagtgt cgtcggggcc ggcggcagca tcgacttggc
     3481 ggcgcctgta ggcggcgcgg caaagatagc ggcgggaacg ctcatcatag cgaatcggat
     3541 cgccggggac gtcgaagcgg ccgtggggac gctgcgcatc acttccagag ccgacatcgg
     3601 cggcaacgtg agttactgga gcggtgagga tgccgctgtt atcgaaggag cacgtatcaa
     3661 cgggaagatc gtcagaaacg tcccgccgca aaggccgcgg atttttccgg ccgtgttcct
     3721 cggctggctc gtattcgtcg gaatcaattt tgtttgtacc ttggtcctgg ggcttctctc
     3781 agctcgtttt ctgccgcgct atcatcaagc cgtcatcact actctcaggg agaagccgtg
     3841 ggcatcgctg ggaataggat ttattgcggc agtcgtggtg ccggtagtct gcgcgctctt
     3901 gttcgcgacc gttctggcag taccgcttgc cctaatactc ctgttcgcgt ttttcattct
     3961 gctctattgg tcgcgagtct acgctattgg cggaatcggt gaatcgatcc ttacccgttt
     4021 ccggccgggg tcaagccgcg cctcggcctt cgtgttggga ctgttcgtct attaccttct
     4081 cgccattatt cctgtcgtcg gatggctcgt ggtccctctg gttatgctct tcggtttggg
     4141 atctgaattg attgcccgga aacagtttta cgtaaccgcg agaagccagg atctaatcta
     4201 acatctcgca aataccccaa ccacgtaaat tagaccttaa acgcagaaaa cgagtcggat
     4261 cccttagatc gccaggcggt aatccgcggt agctctcctg cttcaattct tgctggagct
     4321 tagaaacttc gcttctcccc tgtcattcta agcctcatcg acgtcggtct tgcatttcga
     4381 tctcatattc gacggtctga ctacggtgtt gaacgtgaga gcgcagtcga ctgtttcggt
     4441 gtggccgact tattcggaac tagccatcaa ggagaggcct gaggagtgcc gagaatcggc
     4501 tcccgtttta tctatctaga ggcacccgac gctaagttgt ccaacatcca gattgacact
     4561 catcattgct atggttggcc tgagcagaac gttgacaaag ttaaagagct ggtaaatatt
     4621 gcgggcgaat cggattcgct cgatagagtc tctcacttga taagccaggg cttcgctcca
     4681 tgcatttacg ataactgcgt tagcctcggc gtttggggca tccatgcagc gcattatctt
     4741 accgaccggc atcacagtct cttatgccaa gtacggcgag ggtccgcccc ttgtcctcgt
     4801 gcacggcgcc ttcagcgact acgagaccaa ctggcagttt gttaagcacc tgtttgagaa
     4861 acagttcacc gtccatgcca tcgcgcgccg cggccggggt gaaacggatg caaccaaagg
     4921 ccacagcctt gacgacgagg gcagggacgt cgtagctctg gttcagtcaa tcggcgaacc
     4981 ggtttttctc ctgggtcact cgtacggggc tcataccgcg ctggccgcca cggcccaact
     5041 cccgaattcg gttcgcaagt tggtgctata cgaaccggct tgggcgcgcg ttgtctctaa
     5101 ggatgcgtta gcgcagcttg agaagctcgc gcaagccggc gagtggcaag aattcgcgat
     5161 gagtttcttc cgcgataggc tgttcgttcg ggttaatgag ctggacgagc tacggaccac
     5221 ggacctttgg ccgccgatga ttgccgatgc tcaggcctcg ctaggtgatc tgcgggcgct
     5281 gagtagatat gagttcaacg ccgaggcctt tcgcggcctg cgaatgccgg tgatgctcca
     5341 gatcggcacg gaaagtcctc gagagctcta cgtcaccgac gccctcgccg ccgtccttgc
     5401 cgatgtccgc atcgaggagc tgccggggca agcacacgaa gcgatgacca ccgcaccgcg
     5461 gatgtatgcc gaagcggtat cgcgctttct actcggagag gcgcgttgag attgctgccg
     5521 ccattccaga gctgatgtgg tggacttatc gcaggccaca atccaagacc cttcggggat
     5581 aatcgtcaac ggtttggagc gcggcttgcg cagggttgac gatgaacagc gagtaattgt
     5641 cagtgagtgt ccggcatgag ctattgactt acctccagtt tcccccatgt agggcttctg
     5701 gatttttgcg tcactatcac gtcgcgggtg actagactcg gatcgtggtt agaaacaatt
     5761 ctaaatcagc ggttggctgg tgtgacaccg cgtctttcga ttgccatacc ggggcgcaat
     5821 tgtccatgat ttaagttgac atgtcatata gcgatgaata atcttcgcct cggatggcca
     5881 tcgcgcagat atacaccgac tatagagcac gtagagagga caccgcatat gaacaaatta
     5941 caagaggctc acgtacctta taactccggc tgggccggag agttgaaaac gggccaggtg
     6001 attcggatca tcgcgacgac taccgtcgat ttcgtgtgca tgagacggga aaatctcaaa
     6061 gagcgattcg accaggcgcg cactaaggtc tataacatga aaatctttct caccaccggc
     6121 gacaagctga tggggcgaaa taatcagcac atgatgacca tcgtcgataa caccaatcgc
     6181 gagggaactc acgatttgca gaagggcatg tgcagcggtt accgcttaca actcgcaaaa
     6241 aatgagggaa agctgcggga ctattaccac cgagactata aggacgaaga gattccggat
     6301 catggctgct acgaaaatct ttcgcgagct ttagctccgt acgggatcga tccagaagac
     6361 gtccccaatc cttttaacct caatcagcat atgaaaatcg acggcgccac cggccgcatg
     6421 gatcatacga cagtgcggcc caaagcgggc agctacgtag atcttaaagc agagatggat
     6481 ctgctcgttg ccttcagcgc ctgtcccgat atgccggttg gcggaaagcc ggtcgatgtt
     6541 gtgatttacg agccgtaaaa tagtttgagt tttgcgcctt gaatgggacc tggagaagcg
     6601 atcgctccag cctgatatca gcaaagccat tgcgtctagt aatcgcgcgg cggccaacaa
     6661 ttcagggcga cgtagctact aatcggctcg ccataacatt tctttcacac acgagatcag
     6721 gatgaacacc atgaagcacc gctacagcgc tgagatccag cggagcaggg ggctgctgga
     6781 gctggctgca gctctctacg acacctctct ctcttttcaa accgagcgcg ccgccgagca
     6841 ggcgatgcag accgaatcgc gcgtcggcta ttcgctcacg cttgcgctta ccgcgacccg
     6901 cgaaaaaggc gtttccttgt cgtttgcggc ggacggattt cgcgagcttc tggcggtcag
     6961 ccagggcaag ctctcgctcg gctggatcaa tccttcggtc gccgcaacca tggcgttcag
     7021 gggcaaaggg ctgttttcca agcgccagcc gatcaggacc atcgcggtat ttccttcgta
     7081 cgacgttatg ggatttgccg tgcacgagtc t
//
LOCUS       DASUTG010000915         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1623087, whole genome shotgun sequence.
ACCESSION   DASUTG010000915 DASUTG010000000
VERSION     DASUTG010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623087"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF089_21230"
     CDS             complement(<1..413)
                     /locus_tag="VF089_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176539.1"
                     /translation="MGWIDADAHVVESPHTWDYLTPSENKFRPMLFKPEGDTQRAHWV
                     IDGKIRGLFRFTFSKEDLERRSAEIGRDMATTMATRDVADVSARLKHMDELGIDVQVL
                     YPSIFLDQCTERPDTDVALCGAYNRWLADVWKQSEG"
     gene            complement(492..1256)
                     /locus_tag="VF089_21235"
     CDS             complement(492..1256)
                     /locus_tag="VF089_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6176540.1"
                     /translation="MGLMNEKVVVVTGGGNGIGRAYCQGIAKEGGAVVVADIDGAAAE
                     GAAKAIADSGGKALSVQVDVANSASCLEMAKIVLDKFGKIDGLINNAAIFMSVPVTKG
                     GWQDIDEREWDRVMAVNVKGLWLTSRAVVPAMQQRKQGSIVNISSNMAFNGGLTMMHY
                     VTSKAAVVGFSRVLARELGSDNIRVNTLAPGATMSEERASDEALKNYQRSASTRILKR
                     IEQPEDLVGTALYLLSDLSTFVTGQTILVNGGAVLH"
     gene            1527..2522
                     /locus_tag="VF089_21240"
     CDS             1527..2522
                     /locus_tag="VF089_21240"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176541.1"
                     /translation="MNSRLIYLLSFGLISFVLAMPQHLQAKSYIAYISDSTNSSVIYW
                     IAKEAGILKKHGLDLDLIFINGSVRGIQSLIAGDLSYSGAVGTAVINAKLAGADIAII
                     QSQMNTLPYFIIGNAKIKSPEDLKGRSAAVHIPGTSADFAMRLALSKVGIPYSDIKAV
                     TVGGAPARLASVTNGQMDFTVVTDGERIQGEKMGLKVIIDMAKLNVPFQFNCSVTTRT
                     KIRDNPDEVRRVVWSMAESIHFFKTRKEESIKIMQKYTRGLPRHVLEGAHAANSELLV
                     EDTYPTIDGLRNTLEVQTATDPRAAKFKAEDMVELRFVDEMRKSGFVDKLYGRKK"
     gene            2540..>2797
                     /locus_tag="VF089_21245"
     CDS             2540..>2797
                     /locus_tag="VF089_21245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6176542.1"
                     /translation="MIFPQVAAADVAAVATHYDDLDLFYRTIWGTHVHHGYWRTGKES
                     TEEAVENLTHLVAQRAGIKSGNCVCDLGCGYGGSTKIFGEDY"
BASE COUNT          664 a          780 c          698 g          655 t
ORIGIN      
        1 ccttcgcttt gcttccagac atccgccaac catcgattgt aagcgccgca taaagcgacg
       61 tcggtatccg gccgttccgt gcactgatca aggaagatgc tcgggtaaag aacctgcacg
      121 tcgataccaa gctcatccat gtgctttagc cgcgccgaga catcggcgac atcacgagtc
      181 gccattgttg tagccatatc gcggccgatt tcggcggatc ttctttccag atcttctttg
      241 ctgaacgtga agcgaaacaa gccgcgaatt ttaccgtcaa tcacccagtg agctctttgc
      301 gtgtcgccct cgggtttaaa caacatcggg cgaaatttat tttccgaagg ggtgaggtaa
      361 tcccacgtat ggggactttc gaccacatga gcatctgcgt ctatccatcc catgacagcc
      421 ctccatcgtt ccattctaga tttgcaattt ggattttgga ttctaattgt cggccaatat
      481 cttccgcctt ctcagtgcaa tacagcgccg ccgttaacca aaatagtctg accggtaacg
      541 aaggtgctca aatccgacag taaataaaga gcggtaccaa ccaggtcttc cggctgctcg
      601 attcgtttga gaatcctcgt tgatgccgag cgctggtaat tcttcagcgc ctcgtcgctg
      661 gctctctcct cgctcatggt ggcaccgggc gccagagtat tgacgcgaat attatccgac
      721 ccaagctcgc gcgccagcac gcggctaaaa ccaaccaccg ccgctttcga ggtgacataa
      781 tgcatcatgg taagtccgcc gttgaacgcc atgttcgagg aaatattgac gatgcttccc
      841 tgcttccgct gctgcatcgc tggcaccacc gcgcggctgg ttaaccataa ccctttgaca
      901 ttgacggcca tgactcggtc ccattcgcgc tcatcgatgt cttgccagcc gcctttggtt
      961 actggcacgc tcatgaaaat cgcggcattg ttaatcagcc cgtcaatctt accgaatttg
     1021 tccagcacta tttttgccat ctccaggcag cttgccgaat ttgccacatc gacttgcacg
     1081 cttaaagcct tgccgccgct gtcggcaatc gcttttgcag cgccttcggc agccgcacca
     1141 tcgatatcgg ccaccacaac tgccccgcct tctttagcga tgccctgaca gtaagcccgg
     1201 ccgatgccat tgccgccgcc tgtgaccaca acaacctttt cgttcattaa acccatatct
     1261 gttgacctct gttgatccag aattctcgct atcatcgcct cgtgacaact gtcaacaacg
     1321 gctggcacaa ggatcacctg gaatgaaaat cctcggcgct cgcgagccgt gagcgtaaac
     1381 taaacaacca tatagacagc aaagcggatt tgcgataatc acgccaggaa aacacggtcg
     1441 ttagcgaatg actgcggaaa cgtcactatg tagccgtgtt atgtggtatg aatagacagg
     1501 aaaacaacgg accaggaggt tccctgatga acagccgttt aatttatctg ctatcattcg
     1561 gtttgattag ttttgttcta gccatgccgc aacatcttca ggcaaaaagc tacatcgcct
     1621 acatctcgga ttcgaccaat tcttcggtga tctactggat cgccaaagag gcgggtatct
     1681 tgaaaaaaca tgggcttgat ctcgacctga ttttcatcaa cggcagcgtt cgcgggattc
     1741 aaagtttaat cgccggtgac ttaagttata gcggcgccgt cggcacagcg gtgatcaatg
     1801 ccaaactggc gggagcagat atcgctatta tccagagcca gatgaatacg ttgccgtact
     1861 tcatcatcgg caatgccaaa attaagtcgc ctgaagattt aaagggtcga tcggcggcag
     1921 tgcacattcc ggggacctcg gcggatttcg ccatgcggct tgccctgtcg aaggtaggca
     1981 tcccctactc ggatatcaaa gcggttacgg ttggcggcgc gccggcccgg ctggcctcgg
     2041 tcacgaacgg acagatggat ttcactgtcg tcaccgacgg cgagcggatc cagggagaga
     2101 aaatgggctt gaaagtcatc atagacatgg cgaagctaaa cgtgcctttt cagtttaact
     2161 gcagcgtgac cacccgtaca aagattcgcg acaatcccga cgaagtgcgg cgagtcgtct
     2221 ggtcgatggc ggaatcgatc cattttttta agacccgtaa agaagagtcg atcaagatca
     2281 tgcagaagta cacgcgcggg ttaccccgcc acgtgttgga aggagctcat gcggccaatt
     2341 ccgagctgct cgttgaagat acctatccga cgatcgacgg cttgagaaac actctggagg
     2401 tccagacggc gaccgatccg cgcgccgcga aattcaaggc cgaggatatg gtcgagctgc
     2461 gtttcgtcga tgaaatgaga aagagcggct tcgtcgataa actctacggt cgaaaaaaat
     2521 aatttagctt acgttaacga tgatctttcc ccaggtcgcg gccgcggacg ttgcagcggt
     2581 tgccactcac tatgacgatc tcgatctctt ctatcgcact atctggggca cccacgttca
     2641 tcatggctac tggcggaccg gaaaagaaag cactgaagaa gccgtcgaga acctaaccca
     2701 cctcgtcgct cagcgggccg gcattaaatc cgggaattgt gtttgcgatc ttggctgtgg
     2761 ctacggcggc tcgacaaaaa tctttggcga agattac
//
LOCUS       DASUTG010000916         6125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1476686, whole genome shotgun sequence.
ACCESSION   DASUTG010000916 DASUTG010000000
VERSION     DASUTG010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476686"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1233
                     /locus_tag="VF089_21250"
     CDS             301..1233
                     /locus_tag="VF089_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6176543.1"
                     /translation="MTGKLFIGLAVAWLFTTGAAAPVKMTPVKVTDGVYMLEHSQGSG
                     NSTVVITDDGVLVLDFHIDNADQTLAFIRKTTDKKVKYLITSHSAGDHASGAWHFRED
                     APAWIATKNQAHDLQMQEREEFIERKNSKDPRYAAYQKGEPLTPTIAIDGPMTLYFGG
                     LTFQITPEARAHSTGDLTVYIPQKRVMLMGDLLDTEIHPGQGESAGVFFSNVGGWIKV
                     LDNIMARSLAVETYIPGHGPVHIGRGVKDLEEQKRYFVVMRDEVAKMVQAGKTVEQIE
                     KEFKVPQEFAHYKRPERLRQFFKLFYHQLAETGY"
     gene            1326..2387
                     /locus_tag="VF089_21255"
     CDS             1326..2387
                     /locus_tag="VF089_21255"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6176544.1"
                     /translation="MRLKTITFFIALLVLAVGLVSSGSAQTAKPMSLAQLAAYNKPDR
                     EKVLYAGAKAEGQITWYTSLAGGSYKDLAAAFESQYPGVKVESYRGTRQELGARILAE
                     SQAKRHIVDTIETTIPLLKLLKENKLIAPYYFPAESKYPEHVKEKGGKGLVYWAIDRE
                     SHIGLAYNKNSIPANLVPKNYDGLLRPELKGKIGFAGSDTGVTVVGAMLKFKGEEYFK
                     KLNEQNPAIHNVSGRALLDMVISGEVGVSPTTFRNHAEVSLRANAPIEWLPMEIVPSN
                     SGSTAVSANAPHPHAALLLADFILGPGGQKVLEKYEYGSPIKDYGFKRWYPEQGLTTE
                     QYEKLDEVWNKRLRELARR"
     gene            2621..4090
                     /locus_tag="VF089_21260"
     CDS             2621..4090
                     /locus_tag="VF089_21260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6176545.1"
                     /translation="MNTGSYIDGKWFHPKSERLVRNINPADLDDVIAEFPAATSQDAQ
                     RAIEAAQAAFKSWKRTPGPERGRVLWRAADIARQRADEIARTLTREEGKILKEAKGEV
                     LKGISLLEFYAGEGYRMHGKTLPSEARDTFTYTVRRPLGVVGLIAPWNFPWAIPVWKS
                     APALVAGNTVIFKPAELTPATAAMLAEIYEQAGLPPGVFNMVVGSGSVVGEAIVNSPA
                     VRAVSFTGSNEIGGALYVKAAQRGAKVTCEMGGKNAVIVMADADLDKAAVAIHGGAFG
                     STGQRCTATSRVIVNPAIKEALTERLIEAAKKIKIGSGLDETVDMGPAVDEKQWKTDL
                     DYIRIGQDEGARLLLGGKKPEHVGKGYFVEPTVFDNVTPGMRLFKEEVFGPVLSVSTA
                     NNLEEALSYANGVEYGLTTSIFTENIDTIMNFVEEVETGMVHVNEPTIGGEAQLPFGG
                     TKSTGVGEREMAEEGLNFFTELKTVFINYSGKAERLMIR"
     gene            complement(4281..4664)
                     /locus_tag="VF089_21265"
     CDS             complement(4281..4664)
                     /locus_tag="VF089_21265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176546.1"
                     /translation="MAKIRHIAYRAEDVDAMAKFFVDAMGMTIKQKRKNTAVDLTDGT
                     TNITVLPLNAGRPGGEAGRAGIDHIGFTVESEEEASRMLEAAGAHKIGRVELGSQVHY
                     EVKYQGPEGIVVDVGHWLGTEPIEK"
     gene            complement(4829..5332)
                     /locus_tag="VF089_21270"
     CDS             complement(4829..5332)
                     /locus_tag="VF089_21270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX6176547.1"
                     /translation="MDKETKKTALRMIPYGLYVLTGEGKDGKIAASTVNWVTQTAFEP
                     PLVVVGVKADSGAHAIIKESGTFALNILGKDQKNQAYTFFKALEREGDTIGGEPFRKG
                     SCGAPILENAPAYVECQLVETIEKGDHSIFVGEVKDAGVAAQPAGRPDEATLTLKDLG
                     DKVFYGG"
     gene            complement(5579..>6125)
                     /locus_tag="VF089_21275"
     CDS             complement(5579..>6125)
                     /locus_tag="VF089_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176548.1"
                     /translation="HGIEYKRAGPEHYAGIVRLQRVNYIANLSAEERREGFLSAEFSL
                     EQVAAIASDLGIAIVTMDDELAGCLCAIRREFDHGSPVVAKMLECYDQARFEGKPLSA
                     FNSYIYGPVCIVRHYRRRGLLRGLFNFQKKDLAGQFEVGVALVSYSNGHSMQAHVEGL
                     GMTVAGEFELNGKFAILAFRI"
BASE COUNT         1474 a         1710 c         1643 g         1298 t
ORIGIN      
        1 ggcaagtact gaaagccttt gaagaagcca attcgcgcgg cgacggttcc attgccatgg
       61 gcggacagct aatcgatcgg cccatcgttg aacgagcgcg ccggacaatc gacatggcgg
      121 cggcgctcgg catcgatcga tagcaaagaa tgaaagcgaa cgaaatagaa accttttcct
      181 tctgttctcc atgctaagtt gtcactcgca ttccaaccaa gatgttaagc cggaacagca
      241 gcccagaccc acactatcct cgacccatcg caacaatcgc aagcaacagg gagaaattcg
      301 atgacaggga aactcttcat aggactggca gtggcttggc tgtttactac cggagccgcc
      361 gctccggtaa aaatgactcc ggttaaggtt accgatggcg tttatatgct ggagcattct
      421 caaggatccg gcaactcgac ggtggtgatc accgacgacg gagtattggt actggatttt
      481 cacatcgaca acgccgatca aacactggca tttatccgaa agactaccga caaaaaagtt
      541 aaatatctga tcacttccca cagcgccggc gaccacgcgt caggcgcgtg gcacttccgc
      601 gaggacgccc cggcgtggat cgcaactaaa aaccaagcgc atgatctgca gatgcaggag
      661 cgcgaggaat ttatcgagcg caaaaactct aaagacccac gttacgccgc ttatcagaaa
      721 ggcgagccgc tcactccgac tatcgccatc gacgggccga tgacgcttta cttcggtggt
      781 ctcacttttc aaatcacgcc ggaagcccgc gcccacagca ccggggactt aaccgtctat
      841 attccgcaaa aacgtgtgat gctgatgggc gatctcctcg ataccgagat ccatcccggg
      901 caaggcgaat ccgccggcgt atttttctcc aacgtggggg gttggatcaa ggtcctggac
      961 aacatcatgg cgcgcagtct cgccgtcgag acctacattc caggccacgg tcccgtgcat
     1021 atcggccgcg gcgtcaaaga tctagaggaa caaaagcgct acttcgtggt catgagagac
     1081 gaagtcgcaa aaatggtgca agcaggaaaa actgtcgagc aaatcgaaaa ggaatttaag
     1141 gttccccagg agtttgccca ttacaaacgt ccggagcggc tgcggcaatt ttttaaactg
     1201 ttctatcatc aattggcaga aaccggatac tagcgccagc cgcgagagat tgaccacgct
     1261 cgcgaagagc acgataaaca aagctgaagg atgaaaacgg aaagcggaaa cgaggaggaa
     1321 agcgcatgcg tctaaagacg attacttttt ttatcgccct gttggttctg gctgtcggtt
     1381 tagtttcttc cggatcggca caaacggcca aacctatgtc gctggcgcaa ctggcggcgt
     1441 acaacaaacc ggatcgggaa aaggttctgt atgccggcgc caaagccgaa ggccaaatca
     1501 cctggtacac atccctcgca ggcgggtcgt acaaagatct cgccgctgct ttcgaaagtc
     1561 agtatcccgg cgtcaaagta gaatcgtacc gcggcacccg gcaggaactg ggcgccagga
     1621 tcttagccga gagccaagcc aagcggcaca tcgtcgatac catcgagacc accatcccgc
     1681 ttttaaagct gttaaaagag aataagctga tcgcacccta ttattttcca gcggagtcaa
     1741 agtaccccga acatgtcaaa gaaaaaggcg gaaaagggct agtgtactgg gcgatcgatc
     1801 gcgagtctca catcggcctg gcttataaca agaattctat accagccaac ctggtgccaa
     1861 agaactatga cggtttgctg cggcccgagc tgaagggcaa aatcggcttc gccggcagcg
     1921 acacgggtgt gaccgtagtc ggagcgatgc tcaagttcaa aggtgaagaa tatttcaaga
     1981 agttgaacga gcagaaccct gcgattcata acgtctcagg acgagcgctt ctcgacatgg
     2041 tcatatccgg cgaggtcgga gtatcgccga cgaccttccg caatcacgcc gaggtctccc
     2101 tcagagccaa cgctcccata gagtggcttc cgatggagat tgtgccgtca aattcaggat
     2161 cgaccgccgt ttcagctaat gcgccgcatc cacacgccgc actgcttctg gccgacttta
     2221 ttttaggtcc gggcggacaa aaagtgcttg agaaatacga gtacggcagc ccaatcaaag
     2281 actacggatt caagcgctgg tatcccgagc aggggctcac gacagagcag tatgaaaaac
     2341 tcgatgaagt atggaacaag aggttgcgcg agctagctcg cagatagagt aaattccctg
     2401 aaagactcat agctcaggat cacgaacaat tgagcccgcc aagtcggcaa cccgcgagtt
     2461 cggacccagt tttttcgtcg acgcctttgc gcgtcttagc gggagatact ccgaggttga
     2521 attgtcgctt cacgctgtgt tgttcgtgct caatggtctt cttggtccgc gtctcttcgg
     2581 cgaagctata ttacatgcat tatgatccca gggggcaaac atgaataccg gctcctatat
     2641 cgacggcaag tggtttcatc ctaaatccga gcggctggtc cgcaacatca atcccgccga
     2701 tctcgacgat gtcatcgccg aatttcccgc ggcaacatca caggatgcgc aacgggcgat
     2761 tgaagcggca caagcggctt ttaaaagctg gaagagaaca cccggcccag agcgtggccg
     2821 cgtcctctgg cgcgcggcgg acatcgctcg gcagcgcgcc gacgaaatcg cccgtaccct
     2881 cacccgcgag gaagggaaaa tcttgaaaga agccaaaggc gaagtgctga agggcatttc
     2941 gctgttggaa ttctatgccg gcgaaggtta tcgcatgcac ggcaagacgc tcccctcgga
     3001 ggcgcgtgat acttttactt acacggttcg ccggcctttg ggggtggtcg ggctgattgc
     3061 cccatggaac tttccctggg ctattccggt gtggaaatcc gcgccggccc tggttgccgg
     3121 caacacggtg attttcaagc cggctgagct aaccccggcc accgcagcga tgctcgcgga
     3181 gatctatgag caagccggcc tgccgccggg agttttcaat atggtggtag gctccggctc
     3241 ggtcgtcggc gaagccattg tcaattctcc agcggttcgc gccgtatcct tcaccggctc
     3301 caacgaaatc ggcggcgcgc tctatgtcaa agcggcgcaa cgcggcgcca aggtgacctg
     3361 tgaaatgggc ggcaaaaacg cggtcatcgt catggccgat gccgatctgg acaaggcagc
     3421 tgtggccatt cacggcggcg ccttcggttc caccggacag cgctgcacgg cgacatcgcg
     3481 ggtcatcgtc aatcctgcga tcaaagaggc gctcaccgag cggcttatcg aagccgccaa
     3541 gaaaatcaaa atcggctccg gcttggatga gaccgtcgat atgggtccgg ccgtggatga
     3601 gaagcagtgg aaaaccgatc tggactacat cagaatcgga caggatgaag gcgcgcgctt
     3661 gttgctgggc ggtaaaaagc ctgagcacgt cggcaaggga tattttgtcg aaccgaccgt
     3721 attcgataac gtcacgcccg gaatgcgcct cttcaaagag gaagtattcg gacccgtgct
     3781 ttcggtgtca acagccaaca atttagaaga ggcattgagt tatgccaatg gcgtggaata
     3841 cggcctgacc acctccatct ttaccgagaa cattgatacg atcatgaact tcgtcgaaga
     3901 agtcgaaacc ggcatggtgc acgtcaatga acccaccatt ggcggcgaag cgcagctccc
     3961 cttcggcggg accaaatcca caggggtcgg cgagcgcgag atggcggaag aaggcctgaa
     4021 cttcttcacc gagctgaaga ctgtatttat caactattcg gggaaggcgg aacgactgat
     4081 gattcgttaa acgaatcatc agccgttcaa aagttcaaac gttcaagggc agaattcggt
     4141 cgcggtacac atcgttgctc atgatgcgag caattcaact ggtccagacc cggactggtt
     4201 cgtcggaatt gatgaagcct cagggtccgc gttgaactgt tgaactgaac cgttgagcga
     4261 ttataccgca atctgtgtgg tcatttttca atgggttccg tcccaagcca gtgacccacg
     4321 tcgaccacga tcccttccgg tccttgatac ttgacctcgt agtgcacctg gctgccgagc
     4381 tcgactcgtc caatcttgtg cgcgccggct gcctcgagca tgcggctcgc ttcctcttca
     4441 ctttcaacag tgaagccgat gtgatcgatc ccggcacggc ccgcttcgcc accgggtctg
     4501 cccgcattca aaggaagcac cgtgatgttg gtggttccgt cggtaagatc gacggcggtg
     4561 tttttacgct tctgcttgat ggtcatgccc atcgcatcta cgaaaaactt cgccatcgca
     4621 tcgacgtcct ctgcgcggta ggcaatgtgt cgaattttag ccataatttt cccttttatc
     4681 ggatcgtttt tatttttctt gtcggcagcc gatcaaaaag gctagatgcg agacgcgcga
     4741 tgagtggtcg agcgaaagcg tactcctagt gtacgttgaa gcgagacgac cgagcgcaac
     4801 gaagcaggtg gcctttttca tcaggctgct aaccgccata aaaaactttg tcgccaagat
     4861 ctttaagtgt tagcgtcgcc tcgtcagggc gtccggcggg ctgcgcggca accccggcat
     4921 ctttgacctc accaacgaaa atcgaatgat cgcccttctc gatcgtctcc accagctggc
     4981 actcgacata tgccggagcg ttctccaaaa tcggcgcgcc acaacttccc ttgcggaatg
     5041 gttcgccgcc gatcgtatca ccctcgcgtt cgagcgcttt gaaaaaagta taagcctggt
     5101 ttttttgatc tttgccgaga atatttaatg cgaacgttcc cgattccttg ataatcgcat
     5161 gagcgccgga gtcggctttc acacccacta ccaccagggg cggctcaaac gcagtctgcg
     5221 tcacccagtt aaccgtcgac gcggcgatct ttccgtcctt gccttcgccc gtcagaacat
     5281 agagcccgta cggaatcatt cgcaatgcgg tcttcttggt ctctttatcc atattatctc
     5341 ctttcgaaat tgttgccgtc gccaaggagt gacaacgact aaggcgattc tatgagagcg
     5401 ctagccaatg cgcaagccgg tgcaggcttc actagcaggt tgctgaaaag cctcatatgc
     5461 ttcgttgcgc ttcgcctccg ggatcttttt gagcagcctg caaacagagt ttcagcacgc
     5521 cggggcgact caccccgagg gcattggtaa cgtgtgaatg attccggcag ccatagattc
     5581 atatgcggaa ggccaggata gcgaatttgc cgttcaactc gaattctccc gctacggtca
     5641 ttcccaagcc ctcgacatgc gcctgcatcg aatgaccatt gctatacgag accagggcaa
     5701 cgccaacctc gaattgaccg gccaaatctt ttttttggaa gttaaagagc ccgcgcagca
     5761 ggccgcgtcg gcggtagtgc cggacgatgc ataccggacc gtagatataa ctgttgaatg
     5821 cgcttagcgg tttgccttcg aatctagctt ggtcatagca ttcgagcatt tttgcaacaa
     5881 ccggcgagcc atgatcgaat tcacgcctga ttgcgcacaa gcaacccgct agctcatcgt
     5941 ccatcgtgac aatcgcgatg ccgagatcac tcgcgatggc ggcaacctgc tctaaactga
     6001 actcggcgga gagaaaacct tcccggcgct cctctgccga gagattggcg atatagttaa
     6061 cccgctgcag ccgaacaatt ccggcataat gttccggccc ggcccgttta tattcgatgc
     6121 catgc
//
LOCUS       DASUTG010000917         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1476879, whole genome shotgun sequence.
ACCESSION   DASUTG010000917 DASUTG010000000
VERSION     DASUTG010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476879"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF089_21280"
     CDS             <1..213
                     /locus_tag="VF089_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176549.1"
                     /translation="GINLMACYFIVYHHGGKIEVQNQQGQGVTFTLTFPIQPKNVSAA
                     QEEEAFISKVLMNERFWERLISDQIV"
     gene            377..811
                     /locus_tag="VF089_21285"
     CDS             377..811
                     /locus_tag="VF089_21285"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX6176550.1"
                     /translation="MGLHGDALLREARDTIKPLPEQSAKSDDAAPTNFNQLADAVEKA
                     IKAALIENHGSLPAKHDHQKLVAICQNTGVWDVLPPALRTLVQEVEIYRATTLKSTQN
                     SAEPASKREELKKYFGVTRRLIDYMEYHVIGNDSVLRRLKVA"
     gene            complement(1151..1582)
                     /locus_tag="VF089_21290"
     CDS             complement(1151..1582)
                     /locus_tag="VF089_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176551.1"
                     /translation="MTHKLLAVSVFMYHFHASLSSASAQALGEYGRTLGGATQRQGNT
                     VPHSSRGGNVKGKSEGGSRGVGDLGVQPLQNRLVVATNRAPLYQNQDDEAPRVDELPA
                     GAILVPIIQATSGATGWYMVKTPKGTIGWVKATDVLEQSVK"
     gene            1939..2925
                     /locus_tag="VF089_21295"
     CDS             1939..2925
                     /locus_tag="VF089_21295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6176552.1"
                     /translation="MAGQHNEHEKFLVLIPVYDDWKALELLLPLLDQELTAHALRADI
                     LIVDDGSSEFNFLPEKQKCFRSVETIEILSLRRNVGHQRAIAVGLSYLEANQPCYSVV
                     VMDCDGEDSPSDVPRLIAECRAHHDQKIIFAARTRRSASLSFKTFYHLYRLIHFLLTG
                     IQVQVGNFSVIPASALRRLVAVSELWNHYAAAVYKAKLPMALIPAQRNCRLEGSSRMS
                     LVALVVHGLSAMAVFGDRIGVRLLILVSIGMSLVSVALITVVGIRLLTTLAIPGWATY
                     VTGILLVILIQMLIIVLVFAFVILTGRNTVSIIPSRDYVHIADAVRRVYDSK"
     gene            2912..>3096
                     /locus_tag="VF089_21300"
     CDS             2912..>3096
                     /locus_tag="VF089_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 12"
                     /protein_id="HEX6176553.1"
                     /translation="MIRNSETSYIGCELDLFSLAINWKWYVKRRIREYLTGDVLEIGA
                     GIGSTTAAMHDGTAHRWV"
BASE COUNT          759 a          775 c          767 g          795 t
ORIGIN      
        1 gggatcaatc tgatggcatg ctatttcatc gtttaccatc acggcggcaa gatcgaagtc
       61 cagaaccaac aaggtcaggg cgtcacgttc acgctgacgt tccccataca acctaaaaac
      121 gtctcagccg cacaagagga agaggcattt atatctaaag tactgatgaa cgagcgattt
      181 tgggaacgtc tgatttccga tcaaatcgtc tgatgtcgtc agaatctgca gctttactga
      241 tctcgaagcg ggagctcgcc gtcattcctt gaaaatggat cggcatgaag ctctggtcct
      301 actattcttg ccaaaaccct gagacgcctt tatcatagag tggcatcttc aacggaagcg
      361 attatggagg ggtgttatgg gattacatgg agacgcattg ttacgtgaag ctcgtgatac
      421 aataaaacca cttcctgaac agtcggcgaa gtctgacgat gccgctccaa caaattttaa
      481 tcaactcgcg gatgctgtag aaaaagcaat taaggctgct ttgatcgaga atcatggtag
      541 tctgccggca aagcacgatc atcagaaatt agttgctatc tgtcagaaca ctggggtttg
      601 ggatgttctc ccgccggcgc ttcgaacact ggtacaggaa gtcgaaatct accgcgcgac
      661 aacattgaag agcacacaaa actcggccga gcctgcttcc aagcgtgagg agctcaaaaa
      721 gtacttcggt gtgacgcgcc gattaattga ttatatggaa taccacgtaa tcggtaacga
      781 ctctgtgctt cggcgtctaa aggtagctta gcggtcgctc ggtcatcagt tcgaaatcta
      841 gcgctgccct cttgtcatgt aaattcgatc ctgccagtaa ggtcggccaa gtcgaggcgc
      901 gcttttctac aatgatgccc tgagaaaaca aactcaccca ctggcgtgca gggtcctttc
      961 tttaagagcc caccttggct tgtcacgtaa aacatcgaat gtcgtattct gaagcgagca
     1021 gcacggtgac caaaccctac ctgttggatt tatttccata aaaagcatta tctcgtccat
     1081 cgaaagactc ttgcgaccta gccgcaaaca ggcgtggatt gtcgaacgag atctaactta
     1141 gatgccattt ctattttacg gattgttcga gaacatccgt tgctttcacc cagccgatag
     1201 tacctttcgg agtcttcacc atataccagc cggtagcacc gctagtagct tgaattatcg
     1261 gaaccaggat agcgccagct ggaagctcgt ctactcttgg ggcctcatcg tcttggtttt
     1321 gataaagtgg cgcgcggttg gtggcgacta ctaggcgatt ttgaaggggc tgcacaccca
     1381 aatcgccgac gcctcgggag ccaccctccg actttccttt gacattgcca cctcgcgacg
     1441 aatgcggaac ggtgttacct tgccgctgcg tggctccgcc cagcgtcctg ccgtactcac
     1501 ccaacgcttg cgcagaagca gaagacaggg aagcatggaa atgatacata aagacgctga
     1561 ccgccaatag cttgtgggtc atacaccggg ttcctcatca cgtaccgaac taaacgaatg
     1621 ttaccacaac tggtttttgt aagagcatct ttttacaatg tcgcgttagc tttgacttcc
     1681 gcaattcctt caaaggtcgt tatagcgctc tgatacacta tcaacatcct ctaattctgc
     1741 agcaacccct gtcgtagatg atggtgcttt ttcgcgaatg cggattgagc cacatcaggc
     1801 gtggacatca agaacaaaaa ggttaggttt tgtggtaatc tgccggtgcg ctcgacttcg
     1861 tggcaccatc gctacttatg tgcggctaaa cgcagcgcac acaagtagat aattttgatc
     1921 acgtaagaga catttctaat ggcaggtcaa cataacgaac acgaaaagtt cctagtcttg
     1981 ataccagtct atgacgattg gaaggcgctt gaacttctgt tgccgctctt ggaccaggag
     2041 ctcactgctc acgccctcag agcagatatt ctaatagtcg atgatggatc gtcagaattc
     2101 aactttctgc ctgaaaagca aaaatgtttt cgttccgtcg aaaccatcga gatactttcg
     2161 ctgcggcgca acgttggtca tcagagagcc atcgcagtcg ggttatccta cctagaagcg
     2221 aaccagccct gctactcggt tgtcgttatg gattgtgacg gtgaggacag tccaagcgat
     2281 gtcccccgcc ttatcgcaga gtgtcgtgcg catcacgatc aaaagattat cttcgctgca
     2341 cgaaccagac gctcggcaag tttatcattc aaaaccttct atcacctgta tcggttgatt
     2401 catttcttgt tgaccggcat tcaggtacaa gttggcaact tcagtgtgat acccgcgagc
     2461 gctctcagac gactagttgc tgtttccgag ctctggaacc actatgccgc agcggtttat
     2521 aaggcgaagc taccgatggc tctcatccca gcccagcgta attgcaggct cgagggatcg
     2581 tcgcgcatga gcttagttgc tctcgtggtt cacggattga gcgctatggc ggtgtttggc
     2641 gaccgtatcg gagtcaggtt gttgatactt gtctccatcg gaatgagctt ggtcagtgta
     2701 gccttaatta ccgttgttgg tatccgattg ttaacaaccc tcgccatacc cggctgggct
     2761 acgtacgtga ccgggatctt gttagtgata ctgattcaga tgctcattat agtcctggtc
     2821 tttgcatttg tgattctcac gggtcgcaat accgttagca tcatccccag tcgggactat
     2881 gttcacatcg cggatgccgt tcgtcgggtc tatgattcga aatagtgaaa cttcttatat
     2941 cggctgcgag ctcgatctct tctctctcgc gataaattgg aagtggtatg taaagcggag
     3001 gatcagggag tatttgaccg gggacgtact tgaaattggc gcagggatcg gcagcaccac
     3061 tgcggcgatg cacgatggaa ccgcacatag gtgggt
//
LOCUS       DASUTG010000918         7108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1623595, whole genome shotgun sequence.
ACCESSION   DASUTG010000918 DASUTG010000000
VERSION     DASUTG010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623595"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF089_21305"
     CDS             <1..493
                     /locus_tag="VF089_21305"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6176554.1"
                     /translation="TYAWAQSKINYKVIPNLEPMKDQAPTPDFTLPNPDGKKVSLRDF
                     RGKLVFLNFWATWCVPCREEMPAMEKLYQEFKDKNFVILAVNVKDRKQDAVNFAKELK
                     LSYPIVIDADAQVGLLYGAWGLPTTYLIGPKGEGLARAWGPAEWYSPAARNLIKDLLD
                     GKR"
     gene            490..1227
                     /locus_tag="VF089_21310"
     CDS             490..1227
                     /locus_tag="VF089_21310"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6176555.1"
                     /translation="MSDVNVFVAFAAGIFSFLSPCVLPLIPSYLSFISGVSLEDMRQD
                     RTLKRVRARVIFNSVAFIAGFSLVFISLGVSASFLGSLFFGYRNSIRIVGGLFILLVG
                     LYLIGLLRVPALERYLQFNLKDKPAGYLGSVLVGITFAVAWTPCVGPILGAILALAGT
                     SGEIGRGTLLLSTYAAGLGLPFFLSAVGINSFMQFSQSFRRYIHAFHTAGGILLVIVG
                     LLLITDYMTMLNIYALRFTPDWLLKRL"
     gene            1343..1909
                     /gene="folE"
                     /locus_tag="VF089_21315"
     CDS             1343..1909
                     /gene="folE"
                     /locus_tag="VF089_21315"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX6176556.1"
                     /translation="METDNSMEEQIRFILKSLGEDPEREGLVKTPHRVAQALTFLTRG
                     YDMDPAKVINDALFTEDYQEMIVQKDIDFFSLCEHHMLPFFGKAHVAYIPHHKIVGIS
                     KLARLVDVYARRLQVQERLTNQIATIIMEKLDPFGVAVVIEAEHLCMRMRGVEKQNSM
                     VITSTLLGAFQNRQETRNEFMTLIRQRG"
     gene            1921..3399
                     /locus_tag="VF089_21320"
     CDS             1921..3399
                     /locus_tag="VF089_21320"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6176557.1"
                     /translation="MEIKFKDIGPESFKTDLLVLPVSERRLEERSIRALDRRLKGKLQ
                     ERIHKLKFAGTDGTTLLYSTAGVLPASNLLLIGLGKAADIDAETWRRAGARARREASA
                     LGVEEIGFFFSPERNPESAAAAIVEGALMASYQFIKYRSNAKNPLAVKTLTLHKPGLT
                     RNRGMDEAVRTAEQIIGAVFLARDLINEPPSVTTARYLGEEAQRHCRGRGLSVDVWGK
                     KKIESMKLAGLLAVNRGSQEEPRFITIHYKPAVKPRKKIALIGKGITFDSGGLSLKPS
                     KSMETMKLDMAGGAAVIAAMSRLPALRPSVEVIGYIPTTDNLPGPNAQKPGDVIRYLN
                     GKTIEVLNTDAEGRLILADALALAAKQKPDYMINLATLTGACMVALGTQVAGMFSNHP
                     ALAERVMRCAQESGEKLWQLPLVKEYREDIKSSIADIKNIGGGHGGAITAALILQEFV
                     EEIPWAHLDIAGPAYTDRDIVTCPKGGTGFGVRTLLRFLMTV"
     gene            3414..3740
                     /gene="trxA"
                     /locus_tag="VF089_21325"
     CDS             3414..3740
                     /gene="trxA"
                     /locus_tag="VF089_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6176558.1"
                     /translation="MGKITEVGDNNFEVEVIQSTVPVLVDFWAPWCGPCRSIAPIVEE
                     IAGEYEGKLKVTKLNVDDHPATASRYGIRGIPNLLILKAGAVKEQIVGAVPKAKLVSA
                     IDKALQ"
     gene            complement(3854..4957)
                     /gene="rodA"
                     /locus_tag="VF089_21330"
     CDS             complement(3854..4957)
                     /gene="rodA"
                     /locus_tag="VF089_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6176559.1"
                     /translation="MQIDRRLISHFDWPLFLLTLAFVGMGIVTIYSANYDIAAKHAGG
                     LPSRQLLWLGLGLAVMFTTIAVDYHYVDRLAYPFYGLMLLLLILVFFVGHSGGGSQRW
                     INLGFFRLQPSEPAKLAIVLVMAKYFQDSEPSRGYCLRDLWAPFALVGPLILLTLVQP
                     DLGTAIILGLVFMSMMLIGGLRIKSFLGLAAAGLAFLPIGWNLLWPYQRQRVLTFFDP
                     DRDPLGAGYHVIQSQIAIGSGRMFGKGYLHGSQNRLDFLPAQHTDFIFAVFSEEWGFA
                     GCFVLLACYFGMMVYCLKLFERAKDRFGALLIFGMVAILFWHVVINVSMVAGLMPVVG
                     VPLPLFSYGGSALASMMFAVGMMINVSMRRYTF"
     gene            complement(4947..6824)
                     /gene="mrdA"
                     /locus_tag="VF089_21335"
     CDS             complement(4947..6824)
                     /gene="mrdA"
                     /locus_tag="VF089_21335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6176560.1"
                     /translation="MAILGDVARKDGAPELRVRTRFVYLILIVTFLGIMSRLAVLQIA
                     HGERYTYLSENNRVRIKRVPGTRGMIMDRQGELLVDSRPSFDLLFVPEDAGDPQSTLR
                     QLAKYLGRPENELIAVFEENKSRAAFDEIILGRDVDWATVVAVETHQLDLPGVNLRVR
                     PRRNYADGPMAAHVLGYLGEIGSKQLKVLKEHGYGSGDEIGQYGLEKRWEGFLKGKNG
                     GQQVEVDALGRRVRVLHEVVDVPGYTVHLTLDRQLQQTAYEALKGREGTIVALDIHSG
                     AILTMVSTPAFDPNIFARGIKSEEWRSLTKDRLRPLNNRAIQGQYPPGSTFKIIMAIA
                     GLEEGVDPDARISDPGFFPFGNRNFRDWKKGGHGSVDLHKAIVESCDVYFYQLGQRLG
                     IDKIAKYSRAFGLGEKTGAFLDGEKEGLVPDTAWKRKRYRQPWYPGETPSVAIGQGYL
                     TTTPLQLANMMAAVANGGTLYRPRIVNKVESVDGTMVREYGPEKIRTIDLKPITLRRV
                     QAALADVVRGKGGTGGAARSSIVSVAGKTGTAQVIEMKAAYLKSEQLSYFNRDHAWFV
                     AYAPVENPQVAIVVLVEHGGHGGEAAAPMAKKVFEKYIEMKNQPKDQQQARAPGEIGA
                     D"
     gene            complement(6824..>7108)
                     /locus_tag="VF089_21340"
     CDS             complement(6824..>7108)
                     /locus_tag="VF089_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176561.1"
                     /translation="MSLVFLAVYLSSRSIWLHNHLVSAVVVLLASLVKGVALVLVTAV
                     FLSTEGFWMGALRYILLEALVAALLAPFVFALLRRGQTYLEKVQIPGLYY"
BASE COUNT         1718 a         1929 c         1874 g         1587 t
ORIGIN      
        1 gacgtatgct tgggcccaga gtaagatcaa ttacaaagtc attcccaact tggagccaat
       61 gaaagaccaa gcgccaacgc cggatttcac tttaccgaat ccggacggca agaaagtctc
      121 gttaagagat tttcgcggca aactggtttt cttgaatttc tgggcgacat ggtgcgtgcc
      181 ctgtcgcgaa gagatgccgg cgatggaaaa gctctaccag gaatttaagg acaaaaattt
      241 cgtgattctg gcagtcaatg taaaagatcg taagcaggac gccgtcaact tcgccaagga
      301 actaaagctc tcctatccta tcgtcatcga tgcggatgct caggttggcc tactctacgg
      361 cgcttgggga ttgccgacga cctatttgat cgggcccaaa ggcgaagggt tggcgcgcgc
      421 atggggaccg gctgagtggt atagtcccgc cgcccgcaac cttatcaaag atctgcttga
      481 tggaaaaaga tgagcgatgt taatgttttc gttgctttcg cggccggtat tttttcgttc
      541 ctctcacctt gcgttctacc gctaattccc tcgtacctgt cgtttatatc cggcgtatct
      601 ctggaagaca tgcgccagga tcgaacactc aagcgagtcc gcgcgcgcgt tatcttcaat
      661 tcggtggctt tcatcgccgg tttctccttg gtctttattt ccctcggcgt atccgccagt
      721 tttcttggca gcctgttttt cggctatcgc aatagcattc gcattgtcgg cggtctcttc
      781 attctattgg tcgggcttta cttgataggt ctcctcagag tcccagccct cgaacgctat
      841 ctgcagttca atttaaaaga taagccggcc ggttatttag gttctgtctt ggtcggcatc
      901 accttcgcgg tggcgtggac gccgtgcgtc ggaccgatcc tcggggccat tctcgctttg
      961 gcgggaacat cgggggaaat tggccggggc accttgctgc tgtcgaccta tgcggcgggg
     1021 cttggcctgc cgttctttct gagcgcggtg gggatcaatt cttttatgca attctcacaa
     1081 tcgtttcgtc gctatattca tgcatttcat acggcgggag gaattttgct ggtgatcgtc
     1141 ggcctgctct tgatcaccga ttatatgact atgctgaaca tctatgcctt acgtttcacc
     1201 cctgactggt tgctcaagag gctttagccg ccccttcgtc gaactaacaa tatttgacct
     1261 gcttatggct aaatgttagc tttttccgat tacaaaattg cttcaatcgt cgacctcgcc
     1321 agttctctta agccggaaaa caatggagac cgacaatagc atggaagagc agattcgatt
     1381 catcctgaag tccctgggcg aagatccgga gcgtgaaggg ctggtcaaaa caccgcaccg
     1441 agttgctcaa gcgctgacct tcctgaccag aggttacgac atggatccgg caaaggtcat
     1501 caacgatgcc ctcttcaccg aggactacca agagatgatc gttcaaaagg atatcgattt
     1561 cttttctctt tgcgaacatc acatgttgcc ctttttcggc aaagcacacg tggcgtacat
     1621 cccgcatcac aaaatcgtcg gcatctccaa actcgcgcgt ttagtcgacg tttacgcgcg
     1681 ccgcctgcaa gttcaggaac gactgaccaa tcaaatagcg acgattatca tggaaaagct
     1741 cgacccgttc ggagtcgccg tggtcattga agccgaacat ctatgcatga gaatgcgggg
     1801 cgtggaaaag caaaattcga tggtgatcac cagcaccttg ctcggcgctt ttcaaaatcg
     1861 gcaagaaacc cgcaatgaat tcatgacgtt gattcggcaa cgcggttaac cgaagccttc
     1921 atggaaatta aatttaaaga tatcggaccg gaaagcttta agaccgacct tttagtgttg
     1981 cccgtttccg agaggaggct agaagagaga agcattcgcg cgctagaccg gcgtttaaag
     2041 ggaaagctcc aagagcgcat ccacaaactt aaatttgccg gaaccgatgg gactaccctg
     2101 ctctattcga cagccggagt gctccccgcc tcgaatctgt tgttgattgg gctcggtaaa
     2161 gcggcggata tagatgctga aacctggcgt agagccggag cccgggccag acgagaggcg
     2221 agtgcacttg gagttgagga gattggattt tttttctctc ccgaacggaa tcccgagagt
     2281 gctgccgcgg caatcgtcga gggcgcgcta atggcttctt atcaattcat taaatatcgc
     2341 tccaacgcca agaacccttt ggcagttaag accctcacac tccataaacc cgggcttacc
     2401 cgcaaccgcg gtatggatga ggcggtgcgc acggctgaac aaattatcgg cgcagttttc
     2461 ttggcgagag atttgattaa tgagcctccc tcggtcacga ccgcacgata tttaggcgaa
     2521 gaggcgcagc gtcactgccg cggccgtggc ctgtctgtgg acgtctgggg caaaaagaaa
     2581 attgaatcga tgaagttggc cgggctcttg gctgttaatc gcggcagtca agaagagcca
     2641 cgtttcataa caattcatta caaaccggca gtcaaaccga ggaagaaaat cgccctaatc
     2701 ggcaaaggaa tcactttcga ctcaggtggt ctatcgctca aaccgtcgaa gtcaatggag
     2761 actatgaaac tggatatggc gggtggcgcc gcggtcattg ccgccatgag tcgcttgccg
     2821 gcgctccgtc cgtcagttga ggtcattggt tacatcccga ccactgataa cctgccgggg
     2881 cccaatgctc agaagcccgg cgatgtcatt cgttatctga atggcaagac catcgaggtt
     2941 ttgaacaccg acgccgaagg acgtttgatt cttgccgatg cgttggcgtt agccgccaag
     3001 cagaagccgg attacatgat caatttggct actctgaccg gcgcttgtat ggtcgcgttg
     3061 ggtacacagg tcgccggtat gttcagtaac catcccgctc tcgccgagag agtcatgcgc
     3121 tgtgcgcagg aatcgggaga gaagctgtgg caactgccgc tcgtgaagga gtatcgcgag
     3181 gacatcaaga gcagcattgc cgacatcaag aatattggcg gaggccacgg cggcgcgata
     3241 accgcggcgc tgatactaca agagttcgtt gaagagattc cctgggccca tctagacatc
     3301 gccggccccg catataccga ccgcgacatt gtcacttgtc ccaagggcgg aaccggattc
     3361 ggcgtgcgca ccttgttgag atttcttatg accgtttaga aaaggagaca atcatgggaa
     3421 aaattactga agttggcgac aataatttcg aggttgaagt aatccaaagt accgtgcccg
     3481 ttctggtcga tttctgggcg ccttggtgcg gtccgtgccg gtccattgcg ccaattgttg
     3541 aggaaatcgc cggagagtac gaagggaaac tgaaagtaac taaactcaac gtcgatgatc
     3601 atccggcgac cgcatcgcga tacgggatcc gcggtattcc aaatctgctt attctcaagg
     3661 ccggcgcggt taaagaacag atcgtcggtg ccgtgccgaa ggcaaagctg gtgagcgcaa
     3721 ttgacaaagc cctacagtag aaaacgggat tgttgaaggg gcattccggc gagaatctta
     3781 ccgcagcagt ccaccaagac gcttgatcgt ctggatgatc gagcgttaga atttggccaa
     3841 cggacggccc tcctcagaaa gtgtaccggc gcatactgac gtttatcatc atgccgacgg
     3901 cgaacatcat cgacgccaat gcggacccgc catagctgaa tagcggcagc gggacgccga
     3961 ctaccggcat cagacccgcc accatggaca cattgatgac tacatgccag aacagaattg
     4021 cgaccatgcc gaagatcaac aacgcgccga atcggtcttt ggctcgttca aagagcttaa
     4081 ggcaataaac catcatgcca aaataacagg cgagcaaaac gaaacacccg gcaaagcccc
     4141 actcctcaga aaacactgca aaaatgaagt cggtatgctg agccggcaaa aaatccagcc
     4201 tattctggct cccatgcaag tatcctttgc cgaacatcct tcccgagccg atcgcaatct
     4261 gtgactgaat aacgtggtat ccggcgccca gcgggtcacg atccggatca aaaaacgtca
     4321 gcacacgctg acgttggtac ggccacagga gattccatcc aatcggcaga aatgccagcc
     4381 cggcagcggc taagccgaga aatgacttta ttcgcaggcc gccgatcagc atcatactca
     4441 taaataccag gccgagaata atggcggtac caagatcagg ctgcacaagc gtgagtaata
     4501 tcaacggccc gaccaatgca aacggcgccc aaagatcacg tagacaatag ccgcgtgacg
     4561 gctcgctatc ttgaaaatat ttcgccatca ccaggacaat ggcgagcttt gccggctccg
     4621 agggctgcag tctaaaaaaa ccgagattaa tccagcgctg cgatccgcct cctgaatgtc
     4681 cgacaaagaa aaccaaaatc agcagaagca gcatcaatcc gtaaaacgga tatgccagcc
     4741 gatcaacata gtggtagtca accgcaatgg ttgtgaacat taccgcgagt cccagcccca
     4801 gccaaagaag ttggcgtgaa gggagccctc cggcatgctt cgccgcaatg tcgtaattgg
     4861 cgctataaat ggtcacgatc cccatcccga cgaaggccag ggtcaaaaga aatagaggcc
     4921 aatcgaaatg tgaaatgagg cggcgatcaa tctgcaccga tctctcctgg cgctcgggct
     4981 tgttgttgat cctttggctg gtttttcatt tcgatgtact tttcaaaaac tttttttgcc
     5041 atgggagcag cggcttcacc gccgtgcccg ccgtgctcta ccaacacgac gatggcgact
     5101 tgtggattct cgaccggcgc ataggcgacg aaccaggcat ggtcgcggtt gaaataagag
     5161 agttgctcgc tcttgagata ggccgccttc atctcgataa cctgcgcggt gccggtcttg
     5221 ccggcgaccg agacgatgct agatcgcgcc gccccaccgg tgccgccttt gcccctgacc
     5281 acatcagcta gggccgcttg aacccgccgt aaggtgattg gcttcaaatc gatggtgcga
     5341 atcttctcgg gcccgtactc gcgcaccatc gtgccgtcca ccgattcaac tttgttgacg
     5401 atccgcggac gataaagagt cccgccgttg gcaaccgccg ccatcatatt ggcgagctgc
     5461 aacggagtgg tcgttaaata accctggccg attgcaaccg aaggggtttc gcctggatac
     5521 cagggttgtc ggtaacgctt tcttttccat gcggtgtccg gcaccagtcc ctctttttca
     5581 ccgtcgagaa aagcgccggt tttttcgccc aacccgaagg cgcgggaata cttagcgatc
     5641 ttgtctatgc cgaggcgctg gcccagctga tagaaataaa catcgcagga ctcgacgatt
     5701 gccttgtgca ggtcgaccga gccgtgccct cccttcttcc aatcacggaa gttgcgattg
     5761 ccgaacggaa agaaaccggg atcagaaatt cgcgcgtcgg ggtcgacgcc ttcctccagt
     5821 ccggcgatgg ccatgatgat cttgaacgtc gatcctggag gatactgtcc ttgaatcgcg
     5881 cgattattaa gcgggcgcag cctatctttt gtcagggagc gccattcttc agatttaatt
     5941 ccgcgagcga agatatttgg atcgaacgcc ggcgtgctaa ccatcgtcaa gatcgcacca
     6001 ctgtgaatgt ccagtgcgac gatagttcct tccctgcctt tgagcgcttc gtaggcagtt
     6061 tgctgcagct gccgatccag cgtgaggtga accgtataac cgggaacatc aaccacttcg
     6121 tgcagcactc ggacgcggcg tcctaaggca tcgacttcga cctgctgccc gccgttcttg
     6181 cctttgagaa atccttccca tcttttctcc aagccgtact ggccgatttc atcaccggat
     6241 ccgtaaccgt gttctttaag aaccttgagt tgtttggaac cgatctcacc gagatagccg
     6301 agcacatggg cggccatcgg cccgtccgcg taattgcgcc ggggtcgtac ccgcaagtta
     6361 acccccggta gatcgagctg atgggtctcg actgctacaa ccgtggccca atccacatcc
     6421 ctgcctaaga tgatttcgtc gaatgccgcg cgactcttgt tttcctcgaa aactgcaatg
     6481 agctcattct cgggtcgccc caagtacttc gccagttgac gcaatgtgct ttgcgggtct
     6541 cctgcatctt cggggacaaa caacaggtcg aatgatggtc ggctgtcaac caacaactcg
     6601 ccttgacggt ccataatcat gccacgggtg ccgggaactc tcttaattcg cacccggttg
     6661 ttttcagaaa ggtaagtata gcgttccccg tgagcaatct ggagaaccgc caggcgtgac
     6721 atgatgccga gaaaagtgac aatcaagata agataaacga accgcgtacg gacgcggagt
     6781 tccggggcgc catcttttcg agccacatcg ccgagaatag ccatcaataa tacaagccgg
     6841 gtatctggac cttttcgaga taggtttggc cgcggcgcaa gagcgcaaaa acgaacggag
     6901 ccagcaaagc ggcaaccaaa gcttcgagta gtatataccg taaagcgccc atccagaagc
     6961 cttcggtgct gagaaaaacc gccgtgacca acactaacgc gacgcccttg accaatgagg
     7021 ccaagagaac aacgacggcg ctgaccaggt gattgtggag ccagatcgaa cgagaactga
     7081 ggtagacggc taggaatacc aacgacat
//
LOCUS       DASUTG010000919         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_883678, whole genome shotgun sequence.
ACCESSION   DASUTG010000919 DASUTG010000000
VERSION     DASUTG010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883678"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VF089_21345"
     CDS             <1..245
                     /locus_tag="VF089_21345"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX6176562.1"
                     /translation="SSSNPMYGKLVIDGIERGMTPQQALEFALRADKEPHRRQVAVIC
                     RTNGVRLSYCVIVVALADISYLLRSRSAAYAEYSRR"
     gene            570..1571
                     /locus_tag="VF089_21350"
     CDS             570..1571
                     /locus_tag="VF089_21350"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6176563.1"
                     /translation="MRSGNDRRDFLKKSLFFAGAAGLMSSGLGPLTALAQTIGKPMRR
                     ADRYEDTYIFERKPFKWPRNKTLAVWIAPNVEVWHYDLPGGTAISPNVANRVPDVINY
                     AWREYGIRVGLWRVADVLDAAGIKPSVALNSAVCETFPKAMEEMKKRGWEFMGHGTTN
                     SENLAGLAPEKEKEVIQHVLKIIEQSTGKRPRGWLGTGLVQTHNTLDILAEEGVTYCG
                     DWNNDDQPYPMKVKKGKMISIPYCMEINDLPMFVRKNYTGEQYYRSVIDQFDALYADS
                     RKHSRVMGIPLHPMLIGQPLRIKYLQQAIAYMKKHERVWFATAGEIVDAYELANPVS"
     gene            complement(2051..3010)
                     /locus_tag="VF089_21355"
     CDS             complement(2051..3010)
                     /locus_tag="VF089_21355"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6176564.1"
                     /translation="MSRPLRIQYPGAVYHIMNRGRSRRSTFVDDSDYRAFLDTLVEAH
                     RLWGIEVFAYSLMRNHYHLCLRTPKGNLSRIMRHIDGIYTQRFNRRHKRDGTLFRGRY
                     KAILIDADEYLAAVVRYVHLNAVEAGLVKMPEEHRWASHQYYLKSKGIPSWLDTREAI
                     EQIGGTQVFHEFVLSGNEPALRQYYDAKRQNPILGSETFRDRVRQRGQQATREHPRYE
                     RRVVQSAPDRVIQEVMDQYKVTREEIFSGVRGRQNEARKVALYLTKRCCDRTLPEISQ
                     YFGIGSYSTVSWSCRTVTARMSKETNFRDRVERIVMRISQFRW"
     gene            3263..>3834
                     /locus_tag="VF089_21360"
     CDS             3263..>3834
                     /locus_tag="VF089_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176565.1"
                     /translation="MVVIFVATRAAGDPAVLNLPADASQADIDAARERIGTDRSYAVQ
                     FTIFLHGMATFDFGRSIVYGEPVPELIGPRFIASLKLQLASLVLILLTAFPLGVLAAT
                     KRGRLPDRVAQLLAAVGQAAPGFWVGLVLMVIFAVHLRLLPAGGMGTGGLDSWQYFIL
                     PAISTSLFLFSAMTRLLRSSMLESVDAEFV"
BASE COUNT          846 a         1062 c         1047 g          879 t
ORIGIN      
        1 agtcgtcctc caacccgatg tacggcaagc tcgtcatcga cggcatcgag cgcgggatga
       61 cgccgcagca ggcgctcgag ttcgcgctgc gtgccgacaa ggagccccac cggcgccagg
      121 tcgccgtcat atgcaggacc aatggtgtca ggctttcata ttgcgttatt gtagttgccc
      181 tggccgatat ttcctatcta ttgcggtcgc gatctgccgc gtacgcagaa tacagcaggc
      241 gatgatcgat gaaatcacga tgcaaccgca cataagtata aatctcaaag gcatagcgtt
      301 gccgaaactc ttccagtcgc gatccaaatg gtattcccgg tccgcgtcgc cgcggaaaat
      361 cacttggcgc tgattgcccc ggcaaatcac atgataaaac gcgcccgata ttccactctc
      421 ggtttcgtcc catcatcgcc ctgtaacaac ggtccagttc agtaaatcaa tacgaaaccc
      481 tgaccggaat tttccgctag gcaacttcca tcgatgggtg ataggttgga gtcgttccag
      541 ctcacaatcc gtgaaaggag gacaagcaca tgcgaagtgg taatgatcga agagattttc
      601 tcaagaagag tcttttcttc gcgggcgccg cggggctgat gagttcggga ttgggcccgc
      661 tcacggcgtt ggcgcagacg atcggcaagc ccatgcggag ggcggatcgc tatgaggata
      721 cctatatctt cgagcgcaag ccgtttaagt ggccgaggaa taaaaccctc gcggtttgga
      781 tcgcgcccaa tgttgaggtt tggcactacg atttgccggg gggcacggcg atctctccca
      841 atgtggcgaa ccgcgtgccg gatgtgatca attatgcctg gcgcgagtac ggcatccgag
      901 tcggtttgtg gcgtgtcgcg gatgtgctgg acgccgcggg cattaaaccg tcggtggcgc
      961 tcaactcggc tgtgtgcgaa acctttccca aggcgatgga ggaaatgaaa aagcgcggct
     1021 gggagttcat gggtcacggc acaaccaata gtgaaaatct cgccggcttg gcgccggaaa
     1081 aagagaaaga agtgatccaa catgtgctga agatcatcga gcaatcgacc ggcaagcggc
     1141 cgcgcggctg gttgggcacc gggctggtac agacgcacaa cacgcttgac atcctcgccg
     1201 aggagggcgt gacctattgc ggcgattgga acaacgacga ccaaccctac ccgatgaaag
     1261 taaaaaaggg caagatgatt tccattcctt actgcatgga gatcaacgat ctgcccatgt
     1321 tcgtgcgtaa aaattatacc ggcgaacagt actatcggtc ggtgatcgat cagttcgacg
     1381 cgctttacgc cgatagcaga aagcactccc gcgtcatggg cattccgtta cacccgatgc
     1441 tcatcggcca gccgctcaga atcaaatact tgcagcaggc gatcgcctac atgaaaaaac
     1501 atgagcgcgt ctggtttgcc accgccggcg agatcgttga cgcgtatgag ctcgcgaacc
     1561 cggtaagcta aaactccccc ggcaccgcgc agctggaaga gaagttcaac gccccagcta
     1621 cgaaacttta taggcggctc gtagaagaat attccgattc cctttacgcg aatcaggcga
     1681 agacccgtgt ggcgaaattg gagaaagcgc gctagctcaa aaaaatctcc atgcgaaagt
     1741 cactaactca ggcctgaccc tcctgcctga cgttgctcct gccctcccta accaaaacgc
     1801 tttgtaaaac aatgcgtttt tagtggagtg aagcgacgaa tgcgcgcgga cttgaacaat
     1861 atattattgc aagttcccga tgtattgatt gaaacttttg gcgatgcgtt ccatcccctt
     1921 ccttacgcaa actgttgctg atcggcaccc gaaccagtat gtgtgcgctt aggcgtctat
     1981 ccggagaagc caggaggtac aagagatggc tgttcccggt tttcagagct gtaagggtca
     2041 attaaggggt ctaccatcgg aattgactaa tacgcataac gatcctttca actcggtctc
     2101 tgaaatttgt ttctttactc atccgcgccg tcaccgtacg acaactccaa ctcacggtgg
     2161 aataactgcc gatcccgaaa tactggctta tctcaggcaa cgtccggtcg caacaccgct
     2221 tggtcagata cagcgctact ttgcgcgcct cgttctgccg tcccctcacg ccgctaaaga
     2281 tctcctcgcg cgtcactttg tattgatcca tgacttcctg aatcacacgg tccggcgccg
     2341 actggacaac acgacgctca tagcggggat gctctcgagt cgcctgctgc ccccgttgtc
     2401 tgacccgatc gcgaaaggtt tcactgccga gtatcgggtt ttgccgcttg gcgtcataat
     2461 attgcctcag cgctggctcg tttcccgata gtacgaactc gtgaaatacc tgtgttcctc
     2521 cgatctgttc aatggcttct ctggtatcga gccagctcgg tattcctttt gacttgagat
     2581 agtactgatg gctggcccag cgatgttcct cgggcatctt gaccagtccc gcctccaccg
     2641 cgtttaaatg aacgtagcgt accactgctg ccagatattc atcggcatcg atgaggatgg
     2701 ccttatagcg gccccgaaac aatgtcccat cccgcttatg acgccggtta aatcgctgag
     2761 tgtagatgcc gtcgatatgg cgcatgatcc gggagaggtt tcctttcgga gtgcgcagac
     2821 acagatgata atgatttctc atcagagaat aggcaaacac ctcgatgccc cataaccgat
     2881 gggcttcaac aagggtatcg agaaaagctc gatagtcgga atcgtcaaca aacgtcgacc
     2941 gtcttgatct accccggttc ataatgtggt agacggcgcc ggggtactga attctgagcg
     3001 gtcgcgacat gagggatact accgcagaag gctataacta aacaactcta gacttgaccc
     3061 ctatgctcca ttcaataaac gtgcatgtta gccctcgaca attgagcata ttgcacgtaa
     3121 cgttgctgaa cgtgggctag ctacgggtgt tgcttgctcg atcagagggg cgttaccgat
     3181 tcgctggctc gcatcggagg taggtgccat caggtacgtt atcggtcgta tcgctcaagc
     3241 cgttatcacg ctgatcctct tcatggtggt catctttgtg gccacccgcg ctgcagggga
     3301 tcctgccgtg ctcaatctgc cggcggatgc atcgcaggcg gatatcgacg cggcgcgcga
     3361 gcggatcggc acggaccgca gctacgccgt gcagttcact atcttcctgc acggcatggc
     3421 gacgttcgat ttcggccgtt cgattgtcta cggcgagccg gtcccggaac ttatcgggcc
     3481 ccgctttatc gcgagcctga aacttcaatt ggcgtcgctt gttttaatcc tactgacagc
     3541 gtttccactg ggtgtgctcg ctgcgacaaa acgcggcagg ctgcctgatc gcgtcgcgca
     3601 gttgctggcc gcggtcggcc aagccgcgcc ggggttttgg gtcgggctgg ttttaatggt
     3661 gatcttcgct gtgcatcttc gattgctgcc ggcgggcggc atgggcacag gcggcctcga
     3721 ttcatggcag tacttcattc tgccggcgat cagcacctct ttatttctgt tttccgcgat
     3781 gactcgtctg ctgcgctcgt cgatgctgga gtcggtggac gcagagttcg tgaa
//
LOCUS       DASUTG010000920         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_736375, whole genome shotgun sequence.
ACCESSION   DASUTG010000920 DASUTG010000000
VERSION     DASUTG010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736375"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VF089_21365"
     CDS             complement(<1..688)
                     /locus_tag="VF089_21365"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6176566.1"
                     /translation="MATLITGLGLVGTSYAQLALKRGESIVFYDIAPRKDFLANKLGT
                     ANVTVVQRDVRDLPALIETILKNKIDTVIHTAGLIGGKVAQSIYTGMQINVMGTINVA
                     EAVRLTGVKRLVQISTFGVYDRRGGEPGPIEESFHRGPGEPYGNSKVAKELMVEAYQR
                     LYGFELITLRLANVYGVGHFAGGSGGGEMVQNMLQTGIRGGVVKIAQEVARDFEYVYY
                     KDLGRALDKAA"
     gene            1050..1766
                     /locus_tag="VF089_21370"
     CDS             1050..1766
                     /locus_tag="VF089_21370"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX6176567.1"
                     /translation="MSDATPQIIPIGGGGFYRDPENLALERYVLAQTGVSEPRVVFVP
                     TASGEPDHYVASFYAAFLKLQCRPSVLSLFKRTPDLRALLLEQEVIYVGGGNTKSMIA
                     LWRDWGVPELLREAWQSGIILTGVSAGAICWFEHGLTDSFSNGLRPLGCLGFLPGSCC
                     PHYDGEPQRRPSYHRFLASGEIPAGVAIEDWAAIHYKGTEIHQVIGAKPGVRAYSMRS
                     VYGSVQEVPLPVEYLTQAAE"
     gene            complement(1819..2829)
                     /gene="gap"
                     /locus_tag="VF089_21375"
     CDS             complement(1819..2829)
                     /gene="gap"
                     /locus_tag="VF089_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6176568.1"
                     /translation="MTNVAINGFGRIGRLGLRAMLERHKEELNVVAVNDMADLHTNAH
                     LFRYDSTYGIFPGTVQVGEGQLKIDGWDIAVLCEKDPSRLPWRKLGVDIVIESTGLFT
                     SGAQARAHLEAGAKKVIITAPATDEDVTVVLGVNESAYDPDKHHIVSNASCTTNCLAP
                     LAKVLHENFGIERGLMTTTHSYTNDQRILDLMHSDMRRARAGAANIVPTTTGAAKAIG
                     LVLPELKGKLHGLSLRVPTSSVSIVDLVVDLKKPASAVTINDSLKSSADGNLEGILAY
                     CDEPLVSSDFRGNPASSIIDGLCTVQLEEKMAKILAWYDNEWGYSCRVGDLAAFMIER
                     EL"
     gene            3289..4287
                     /locus_tag="VF089_21380"
     CDS             3289..4287
                     /locus_tag="VF089_21380"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176569.1"
                     /translation="MPFRFYNSSLTITLVLSLLLAMGSTVLAASSATKKVSIIFADFS
                     ERSGLLFVAKDQEFFAEHGLDADIVQVRSGPVAISALAANEAQFYIVSATGSSLGAVA
                     AGLDLAYVAGLINKLDGYFVTSAKIRTPEDLKGKAIGVQSIGGGIWMFTQMALDHWGL
                     STERDKIQMRVLGDQSVLAQALSTGLVDGAVLGYAFSQVVQRSGGRVLAELPKLNIPY
                     QGVGLLARRSLINGSPDTVEKTLRALIKANRFIQDKNNQPAVLASLRKWLRLSPNEGG
                     EELYERMRILYDRRITPSREGIQNALRVLSKADPKFAKLKPDDLIDDRIARKLERE"
     gene            4384..>5212
                     /locus_tag="VF089_21385"
     CDS             4384..>5212
                     /locus_tag="VF089_21385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176570.1"
                     /translation="MKKLFLWFIAIAWFQTLSVNRVFSAAPPTKVVISYAVLSEREGA
                     LFVARDQGFFRKQGLDVDLVYVSSAPVALASIAAGDSQINTASVSGAILGARAGGLDL
                     VFIAGLVNRLTGTIVSAPEIRTPAELKGKTIGVTSIGGGNWVFTMLALEHWGLDVKRD
                     NITIRVIGNDAVRAQAMTTGVIHATQLSAYSSAAMLKRQGYRILADLPELGIPYQGTT
                     VFARRSYVNQEPEVVEKILTALVQAIDFIRDPANKAAVMRSLAKGLRLPKPEDTADGY
                     "
BASE COUNT         1104 a         1393 c         1495 g         1220 t
ORIGIN      
        1 tcgctgcttt gtcgagcgct cggcccaagt ctttgtagta aacatattcg aagtcgcgcg
       61 ccacttcttg cgcaatcttt accacgccgc cgcgaattcc ggtctgcagc atgttctgca
      121 ccatttcgcc gccgccggag ccaccggcaa agtggccgac gccatagaca ttggcgagcc
      181 gcaaagtgat cagctcaaat ccatagagcc gttgataagc ttctaccatg agttccttag
      241 cgactttaga gttgccgtac ggttccccgg gtccgcggtg aaaactctcc tcgatcgggc
      301 cgggctctcc accgcgccgg tcgtaaacgc cgaacgtgct gatctgcacc agccgtttga
      361 ctccggtcaa acgtaccgct tcggccacat tgatggtacc catcacattg atctgcatgc
      421 cggtatagat cgactgtgcg actttgccgc cgatcagtcc cgcggtgtgg atgacggtat
      481 cgattttatt tttcaggatc gtttcaatca gcgccggcaa gtcacgcaca tcgcgctgca
      541 caaccgtcac gtttgccgtg cccaatttat tcgccaggaa atctttcctc ggggcaatgt
      601 cgtagaaaac gatgctttcg ccacgcttga gcgcgagttg agcataagaa gtgccaacca
      661 agcccaaccc cgtaattaat gtcgccatag attcctctcc gactccgtga tttattcccc
      721 ttactcctgc aactcggaaa tgtccaggct caatctcgtt catccggcct gtcggcaggg
      781 taaatccgcg tcagagaaac tgtcgcccaa tcggaactgc atgtgatatg aatcgtgagg
      841 aatttgaaag tctgaggagc gactgatgag accaacaaga acgaagtctt ggttctcgcg
      901 cttggcgaaa tcaaccgcgt cgaacaagag gaattcgacc ggatcaaagc acgctatgaa
      961 agtattgccg agcaggccga cgcgagttga ggtagctagg ctcgggcatt cctctcgctc
     1021 gctataagag ctgaccagga gtcaaacacg tgtcggatgc aacaccacag atcattccca
     1081 tcggcggcgg cggattctat cgcgacccgg agaatcttgc gctcgagcgt tacgtcttgg
     1141 ctcaaaccgg cgtttccgaa ccgcgggtgg tctttgtgcc caccgccagt ggcgagccgg
     1201 accattatgt tgccagtttc tatgcggcat tcctgaaact gcaatgccgt ccttcggtgt
     1261 tatcgttgtt taaaaggacg ccggatttgc gcgctttgct gctcgaacaa gaggtgattt
     1321 atgtcggcgg cggcaatacc aaaagcatga ttgccctatg gcgcgactgg ggcgtgcccg
     1381 aattgctccg agaggcgtgg cagtcgggaa ttattctcac gggagtgagc gcgggagcga
     1441 tctgttggtt cgagcacgga ctaacggact cattctcaaa tgggctgcgt ccgcttggct
     1501 gtctcggttt tcttcccggc agctgttgtc cgcattacga cggcgagccg cagcgccggc
     1561 cctcttatca ccggtttctg gccagcggcg agattcctgc tggcgtggcg atcgaagatt
     1621 gggccgcaat tcactacaaa ggcaccgaga tccatcaagt gatcggggca aagcccggcg
     1681 ttcgggctta cagcatgcgc agtgtctatg gctcggtgca ggaagtaccg ctgccggtgg
     1741 aatatctgac acaagccgca gagtaaatcc tcgcccccct aggcctgatg ccaaagttgg
     1801 tcagacgaag gctcttattt atagttcgcg ctcgatcatg aacgcagcga gatcgccgac
     1861 gcggcagcta tagccccatt cgttatcgta ccacgctaag atctttgcca ttttctcttc
     1921 cagttggacc gtgcataagc catctatgat ggaggaggcc gggttgccgc ggaaatcgct
     1981 ggaaaccagc ggctcatcgc aataggccaa gatgccctcg agattaccat cggcagagct
     2041 cttgagtgag tcgttgatcg ttactgccga ggctggcttt ttcaggtcga ccaccagatc
     2101 gacgatggaa acactggatg tcggcactct gagcgagaga ccgtgaagct tgcccttgag
     2161 ttcgggcagg accagtccga ttgctttggc ggcgcctgtg gtcgtcggta cgatgttggc
     2221 tgcgccggcg cgggcgcggc gcatatcgct gtgcatcaga tcgagaatcc gctgatcgtt
     2281 ggtgtaggaa tgcgtggttg tcatcagacc gcgctcgata ccgaagttct cgtgtaagac
     2341 tttggccagc ggagcgagac agttggttgt gcaggacgca ttcgagacga tatggtgctt
     2401 gtcagggtca taggccgact cgttgactcc caacaccacg gtcacgtctt cgtctgttgc
     2461 gggcgcggtg ataatcactt tcttcgcccc cgcttccaaa tgagctcgcg cttgagcgcc
     2521 gctggtaaag aggcccgtgg attcaatgac aatgtccacc cccagcttgc gccaaggcag
     2581 ccgcgatgga tctttctcgc acagcacggc aatatcccag ccatcgatct tgagctggcc
     2641 ttcgccaact tggacagttc ccggaaagat tccataggtt gagtcataac gaaacaggtg
     2701 agcgttcgtg tgcaaatcgg ccatgtcgtt tacggcgacg acgttgagtt cttctttgtg
     2761 acgctcgagc attgcgcgca gtccgagacg gccgatccga ccgaatccgt tgatcgctac
     2821 attggtcatt tgtcctcctg cagatttttg gggcgaaggt cttgtggatg tgaatgcaag
     2881 agttaaagca aagtttattc cgttggatga atctatgtca ccggcgctgc ttacctatga
     2941 gcagagcttg agccgctttg ccgatgacaa aaatctaagc ggtggatgtg agatttttgt
     3001 gcacaataga aaagaattgt acatgtttga catatcgtgg cacgagctaa gttgatcgat
     3061 taccctgcaa aaatttgttg gaacgaaggc gctaaaagat ttgtgagtgg gcatcaccaa
     3121 ataaattgcc ttccatggac cggctggtca acgagaccga ctggacttga tggaaacggc
     3181 aaatcatcgc tggcaatcgc gaatcgttgc attcttatgc tcgatcgggt tattgcaatg
     3241 gttttctcac cgcagaactg ctctcgactt tcaaggagga agtgcgtaat gccgtttcgg
     3301 ttttataact cgtcgctaac aatcactttg gtgctctcgc tgctgctagc catgggctca
     3361 acggtcctcg cggccagcag cgccaccaag aaagtgagta ttatctttgc cgacttcagc
     3421 gaacgcagcg ggcttttgtt tgtcgccaag gaccaggagt tttttgctga gcacggattg
     3481 gacgcggata tcgtgcaagt gcgcagcggt ccggtggcga tctcagcttt ggcagccaac
     3541 gaggcacagt tctatattgt ttcggccacc ggctcatccc tcggcgctgt ggcggcggga
     3601 ttggacctgg cctatgtcgc cggtctgatc aataagctcg atggctattt tgtgacctcc
     3661 gcaaagattc gcacgccgga ggacctcaaa ggcaaagcca tcggcgtgca gagcattggt
     3721 ggcggcatct ggatgttcac acaaatggcg ctggaccatt ggggcctgag cactgagaga
     3781 gacaaaatcc agatgcgcgt tctcggcgac cagtcggtgt tagctcaggc gctttcgacg
     3841 ggattagtcg atggcgcggt attgggctac gcattcagcc aggtcgtgca acgcagcggc
     3901 ggccgggtac tggccgaatt acctaagctg aatatcccgt atcagggggt cggcttgctt
     3961 gcccggcgca gcttgatcaa cggttctccg gacacggtgg agaagactct tagagcactg
     4021 atcaaagcca atcgcttcat acaagacaag aacaaccagc cggcggtgct ggctagtttg
     4081 cgtaaatggt tgcggctttc acctaatgaa ggcggcgagg agttgtatga gagaatgcga
     4141 attctttatg accgccggat taccccgagc cgcgagggta ttcagaatgc cttgcgtgtg
     4201 ctgagtaagg ccgatccgaa atttgcaaag ctcaaacctg atgatctcat cgacgatcga
     4261 atcgcgcgca agctcgagcg ggagtgatcg gcagtggcaa taggcgcgtt gatccctcgg
     4321 tcctgcggcc atccggcgtc gctgcctcag gcacctttaa cttgccatga aagctggatc
     4381 tttatgaaga aactcttctt gtggtttatt gctatagcgt ggttccaaac tttgtcggtc
     4441 aacagggttt ttagtgccgc gccaccgaca aaggtcgtga tcagctatgc ggtattgagc
     4501 gagcgtgagg gcgcactctt tgtcgcgcgc gatcaaggat ttttccggaa gcaggggctg
     4561 gatgtggatt tagtttatgt atccagcgct cctgtggctt tggcgtcgat cgccgctggc
     4621 gattcgcaga tcaataccgc ttcggtttcc ggcgccattc tcggcgcaag ggccggcggc
     4681 ttggatctgg ttttcatcgc cggtctggta aaccggttga ccgggacgat tgtttccgcg
     4741 cccgagatca gaactcccgc tgagctgaag ggcaagacca ttggggtgac gagcatcggc
     4801 ggtggtaatt gggtgttcac gatgctcgcg ctcgagcact gggggctcga tgttaagcgc
     4861 gacaacatca ccatccgggt catcgggaac gatgcggtcc gcgcgcaggc gatgaccacc
     4921 ggagtgatcc atgcaactca gctgtcggcg tattcatccg cagccatgct gaaacgccag
     4981 ggttaccgca tcttggctga tctgccggag ttgggaattc cgtaccaggg tacgaccgtg
     5041 tttgcccggc gcagctatgt caatcaagag cccgaagtgg tagaaaaaat tctaaccgcc
     5101 ctcgtccagg ccatcgattt cattcgagac ccggccaata aggcagcagt catgcgcagt
     5161 ttggcgaagg gtcttcgcct gcctaagcct gaggatacgg ccgatggcta tg
//
LOCUS       DASUTG010000921         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2070134, whole genome shotgun sequence.
ACCESSION   DASUTG010000921 DASUTG010000000
VERSION     DASUTG010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070134"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..583
                     /locus_tag="VF089_21390"
     CDS             101..583
                     /locus_tag="VF089_21390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX6176571.1"
                     /translation="MLVGKISLLLVVLSIAVGVVSCQKDDSAKPAAGGHDGGHGTPKD
                     WKFTLGKGDAAEGRRLFVEIECHKCHEIKGESFPSVAADEKDVGPELSQMAGMHPIEF
                     FAESIANPDAVIDADAKEQGYVGTDGKSKMPGFADVLTVKQLTDLATYLASLGGGHKH
                     "
     gene            complement(939..2201)
                     /locus_tag="VF089_21395"
     CDS             complement(939..2201)
                     /locus_tag="VF089_21395"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176572.1"
                     /translation="MTWPRRIKTISPLKWRALESLNYREFRLLWYGQVFTAMATWMDS
                     IARGWLIYELTNSSFQLGLVRGVQAIPTLLLSPIAGSTADLYSRKTQILIAQVVDGLL
                     YAWVAVMIITGDIQPWHVYLTSLGLATVQAFQLPSRAAMVADAVPPSCLTNAMGLNAV
                     VFNSARSAGPALAGVLIALYGTAGSYSIQALFFLLATVWTIMLRPKQRLSRVAKNPAE
                     HPESFRQNILEGWKFSWRNYEVRAGLLVVSAAMFLLIPFTTLLPVFARDILQVGARGQ
                     GILLTCMGGGALFSSVLVAFLGDRMPRGLVMIGGVALYGILLVMFAASSSFVLSMALM
                     FFVGLCHVTSHALIQIVVQFYSPPEFRGRTMSIFHMTQVLLVVGAMFIGSLATFIGAR
                     WATAVLGVIGALAMTAIYTLMPRARRIR"
BASE COUNT          525 a          650 c          682 g          405 t
ORIGIN      
        1 tagatgtgtg ctcgccaaac gcctacggcg tcggggtatc gcggtgaagt gacaacgtgt
       61 tcgtgatgat ctaaagagct ggttaagcgg aggttgattg atgctggtcg gtaaaatttc
      121 cttgctcctg gttgtgttgt cgatagcagt gggcgtcgtt tcctgtcaga aggacgactc
      181 ggcaaagcct gctgccggcg gtcacgacgg cgggcacggc acaccgaagg attggaagtt
      241 cacgctcggc aagggtgacg ctgccgaggg gcgaaggctc tttgtcgaga tcgagtgcca
      301 caagtgtcac gaaattaaag gcgaaagttt cccgagtgtg gcggcggatg agaaggatgt
      361 cggaccggag ctttcgcaaa tggccgggat gcaccccatt gagtttttcg ccgagtccat
      421 cgccaacccc gatgcggtga tcgatgccga cgcgaaagag cagggatacg tcggcaccga
      481 cggcaaatcc aaaatgcccg gtttcgcgga cgtgttgacc gtgaagcagt tgaccgacct
      541 ggcaacctac ctcgcgtcgt tgggcggcgg gcacaaacat taacagccaa atcaaaagac
      601 tgtctaccgt cccttgcggg tcaatttgca gaagagcggc cgcttatttc aggtacgggt
      661 cgagaccgtg ccgggccaac tgcagacctc gagcttcgca tcagcctagg ccatcgccga
      721 attcatgctt aacctgcttc ctatgggcga gccgccgtcg cgtgacactt ggcggagtat
      781 gtgccagcga acttccaacg ggcaaacggc gcattccaat tctcccagga ccaggacttt
      841 gcccggatat cgagaagtga ccacgctgcg tgagcctaat tctcaaacgc gacgcgtcgc
      901 atgctctatg attttaacca ctccgttctt catccacgtc atcgtatacg ccgcgcccgc
      961 ggcatgagcg tgtagatggc cgtcatcgcc agcgcgccga ttacgcccag cacggcggtc
     1021 gcccagcgcg cgccgatgaa ggtggccagg ctgccgatga acatcgcgcc gacgaccaaa
     1081 agcacctgcg tcatgtggaa gatcgacatt gtccgtccgc gaaactccgg aggagaatag
     1141 aattggacga cgatctggat tagcgcatgg gaggtcacgt ggcacagacc gacaaagaac
     1201 atcaacgcca tggacaggac aaaagaggaa gacgcggcaa acatcacgag caatatgccg
     1261 taaagagcga cgccgccgat catgacgagc ccgcgcggca tgcggtcgcc gagaaacgcc
     1321 accagcaccg aactgaacaa agcgccgccg cccatgcagg tgagcaagat cccttgtccc
     1381 cgggcgccga cctgcaggat atcgcgcgca aataccggca gcaatgtagt gaacggaatc
     1441 agcagaaaca tggccgcgga tacgaccaga agaccggccc gcacttcata gtttctccag
     1501 ctgaacttcc agccttcgag aatgttttgc cggaaagatt ccgggtgttc ggcaggattc
     1561 tttgccaccc ttgataagcg ctgcttcggg cggagcatga tcgtccaaac ggtcgccagc
     1621 agaaagaaaa gcgcttgaat ggagtacgag cccgcggtgc cgtacaaggc gatgagcacg
     1681 cccgcgagcg ccggcccagc gctacgcgcc gaattaaaaa ccaccgcatt gagccccatg
     1741 gcgttggtca ggcaactcgg cggcacggca tcggcaacca ttgccgccct cgacggcagt
     1801 tggaacgcct gtacggtcgc gaggccaagc gaagtcaaat acacatgcca tggttgaatg
     1861 tcgccggtga tgatcatcac ggccacccag gcgtagagca agccgtcgac cacttgcgcg
     1921 atcaggatct gcgttttacg tgagtagaga tcggcggtgc tgccggcgat cggcgataac
     1981 aataacgtcg gaattgcctg gacgccccgc accagtccga gctgaaaaga cgagttggtc
     2041 agctcgtaga tcagccagcc gcgcgcgatg gagtccatcc aggtggccat tgcggtgaac
     2101 acctggccgt accagagaag gcgaaactct cggtaattga gcgactccag cgcgcgccat
     2161 ttgagtggag aaatcgtctt aatccggcgc ggccacgtca tatgcgggcg gatctgctaa
     2221 ggtggggccg acaaagatcc gcgccgcagt cctctttcga ta
//
LOCUS       DASUTG010000922         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1773940, whole genome shotgun sequence.
ACCESSION   DASUTG010000922 DASUTG010000000
VERSION     DASUTG010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773940"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VF089_21400"
     CDS             complement(<1..959)
                     /locus_tag="VF089_21400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6176573.1"
                     /translation="MTMHSLYRWLIVIVLSMLLDSPVLAQGVNIDAARKEGKVIAYGT
                     IIPQVMEPLHRNLEKKYGVKVEYWRASATQVMERARSEWLAGRPGFDVTFAINGAQAI
                     LKQAGVFTKYTPASAEKFPAAFKDKEGLVTAWRHTPIGVLYNTDSVPAADAPKSLDDL
                     LHPKWKGKIVLPDPTRHTSTAQFLVSLRKIKGEGWIDYVKALAKQAPLLKESFAPVPN
                     EILRGEATLGFTYLQYVYQNKGPLGYVLMDKILTDTNDLGLSAKAMNPNAAKLYMEYL
                     CSPEGQKLVADLGEFVLSPGIYPPVKDADKITANSIFMDNPSA"
     gene            complement(1258..2457)
                     /locus_tag="VF089_21405"
     CDS             complement(1258..2457)
                     /locus_tag="VF089_21405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6176574.1"
                     /translation="MPTNVIMPALGVAQEKGTLVHWLKAEGQTVNKGEPLMEVETDKA
                     TVEVEAVASGILVNVTAAPGDEVPVGQTIALILTPGETLPEASIAHSLPSAKQNPTPA
                     SAQLKPETPMRTPSPMARVSGSHILASPAARRIAREEGIDLGGLRGSGPEGSVLIADV
                     RQAKEKQLLGPGGAPPVSRLVTISPMRRIVAARMLQSKQSVPHFYISMDIDMTAIVVT
                     RTKWKESGERTFPSTNHFILSACARALREFPSLNARFIHDGIELLPDINIGMAVALAE
                     GLVVPVIRNADQLSLTDLAQQSRQLVEKAQSKKLLPLDCEGGTFTVSNLGMLGVDSFI
                     AIINPPQCAILAVGRVAPRAVADEVGIAVRSMMTATISADHRIVDGAIAARFLADIKR
                     RLENPDC"
BASE COUNT          593 a          791 c          739 g          648 t
ORIGIN      
        1 tccgctgaag gattatccat gaagatgcta ttggcggtga ttttgtcggc atccttgacc
       61 gggggataga tgccgggcga caacacgaac tcgccgagat cggccaccag cttctgtcct
      121 tccggggagc agagatattc catgtagagt ttggcggcat tgggattcat cgccttggcg
      181 ctcaaaccga gatcgttggt gtcggtgagg attttgtcca tcaaaacgta acccaacgga
      241 cctttgttct gatagacgta ctgaagatag gtaaatccga gcgtcgcttc gccgcgcaga
      301 atctcgttgg gcaccggggc aaatgattct ttgagcagcg gcgcctgctt ggctaacgct
      361 ttaacataat cgatccagcc ttcacccttg atcttccgca ggctcacgag aaattgcgcc
      421 gtgctggtat ggcgcgttgg gtcgggaagg acgatctttc ccttccattt cgggtgcaaa
      481 agatcgtcga ggcttttcgg cgcatccgct gccggaaccg aatccgtatt gtaaagcaca
      541 ccaatcggcg tgtggcgcca cgcggtgact aacccctctt tatctttgaa cgcagcggga
      601 aacttttcag ccgacgccgg cgtgtacttt gtgaagacgc cagcctgctt gagaatagcc
      661 tgagcgccgt tgatggcaaa agtcacatcg aaaccggggc gacccgccag ccactccgag
      721 cgtgcgcgct ccatcacttg agttgccgac gcgcgccagt actctacttt gacgccgtat
      781 tttttctcga gattacggtg cagcggctcc atcacctgcg ggatgatggt cccataggcg
      841 atcaccttgc cttctttcct ggccgcgtca atgttgactc cctgtgcgag aacaggtgaa
      901 tccaacaaca tggaaagcac gataacgatt agccaccggt agagcgaatg cattgtcata
      961 ttcacacctc cattgcgatc cgagcccagt tgcccgtgaa tatcgcacag cggattaccc
     1021 gaacacaata atctcttctc tactgtccac ggccttcgtt gccgctatgg gtgggagcgc
     1081 attctaaagc gacctcagac actttcagtg gtgagtccaa cgccactcgc attagctcat
     1141 agagtatacg gcttcgcaca agaacgtttg ctgcacgtcg atccggttat cgatgctcat
     1201 ttgaggacat cggctaagcg tgctgagctt tcggatggcg attttggtca cgcgcaatca
     1261 gcaatcagga ttttccaagc gccgcttgat atccgccagg aagcgcgccg cgatggctcc
     1321 atcgacgatg cgatggtcgg cagatattgt ggcggtcatc atcgatcgaa cggcaatgcc
     1381 gacttcatcg gcgacggcac gcggcgcaac gcgccccacc gccagaatag cgcattgcgg
     1441 tggattgatg atggcgatga aagaatcgac accgagcatt cccagattcg agaccgtgaa
     1501 agttccgcct tcgcaatcaa gcggtaataa ttttttgctt tgcgcttttt caaccaattg
     1561 ccggctctgt tgcgccaggt cggtcaggct cagctggtca gcgttgcgga taaccggcac
     1621 caccagacct tccgcgagag cgactgccat gccgatgttg atatccggca gaagctctat
     1681 tccatcatgg ataaatcttg cattcaggct cgggaactct ctcaaggcgc gagcgcaggc
     1741 gctcaagatg aaatggttgg tcgaaggaaa ggttctctct ccgctctcct tccacttggt
     1801 tctggtcacg acgatggccg tcatgtcgat atccatgctg atatagaaat gcggcacgct
     1861 ctgtttgctt tgcaacattc gcgcggcgac aatccggcgc atcgggctta tcgttaccag
     1921 ccggcttacc ggcggtgcac ctcctggccc cagcaattgc ttttctttgg cctgccgcac
     1981 atccgcgatc agcacgcttc cctccggccc gctgcctctc aaccctccga gatcgatccc
     2041 ttcctctcgc gcgattcttc gcgccgccgg tgaagccaaa atgtgcgagc ccgaaacccg
     2101 cgccattgga ctcggcgttc tcattggagt ctcgggcttg agctgagcag aagccggtgt
     2161 gggattttgc tttgcggaag gtaacgagtg tgcaattgac gcctcaggta acgtttcgcc
     2221 gggcgtcaag atcagcgcga tggtttgacc gaccggcacc tcgtcaccag gggccgcggt
     2281 cacattgacc agaattcctg aagccacagc ttcgacttca acggtcgcct tatcggtctc
     2341 gacttccatc aacggttcgc ctttgttcac cgtctggcct tcggccttga gccagtgtac
     2401 caacgtgcct ttttcctgag cgacgcccag agccggcatg ataacattcg tcggcataaa
     2461 attagttcaa cggttcgatc gtttaaagct caacagtcac ggttattctc tgcatgcggc
     2521 cgacaacatt gatgctcttg aagtcagggc tgagcgagat cctcaacgca gtgaactctt
     2581 gcctagttta gtatcgggta ggagagatga tggaaatcgc agcgtactaa gcgtcacaat
     2641 cgaatctccg tcgcgtccca agacaggcat tagcacggat ccgctgtagt gattgcacgg
     2701 cagtatgtaa cccataatag ccattggaac aaggttcaca gatgagtccc agcgcccaaa
     2761 gtggagcaac c
//
LOCUS       DASUTG010000923        10723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_292509, whole genome shotgun sequence.
ACCESSION   DASUTG010000923 DASUTG010000000
VERSION     DASUTG010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292509"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VF089_21410"
     CDS             complement(<1..1532)
                     /locus_tag="VF089_21410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6176575.1"
                     /translation="MRRTLQTSHVRLAVAKILFLAIFLVIGGRAFYLQAVQGDRLMRL
                     GQRQHLKEWTVLPKRGGVFDRGGEALALSLEAQSVYARPRRIEDASKSARDLARVLKI
                     STAEIKERLTSAKPFVWIKRQISPDEAGQIQNLKIEGVGMFHEPHRYYPQGQLAGQVI
                     GFVGHDSQGLEGLELRYNEYIRGETGSSVSERDALGRRVLVEGVEGLQIPAGGDIHLT
                     LDTSIQHLAEKELEASIDKYRAKAGVAIAVEPRTGEILALANYPTFNPNNYSAASPEQ
                     RRNRAVTDSFEPGSTFKTILAAAALEEGIVDKEDLFFCEYGKFSYAGRIIHDTHPHGW
                     LPFSKILQVSSNIGFSKVADKLKKDRFFKYIEKFGFGQVTGIDMPGEVPGLLRRPETW
                     SGIDLATHAFGQGLSATPLQMVMAYAAIANGGFLMRPYVVRRAVGPKGEVMVENQPHV
                     VRRVISERTASSLASMLQDVTTEGGTGTMAKVEGFNVAGKTGTAQKVDLVNGGYDAKK
                     RVA"
     gene            complement(1529..1891)
                     /locus_tag="VF089_21415"
     CDS             complement(1529..1891)
                     /locus_tag="VF089_21415"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX6176576.1"
                     /translation="MRTKTATAVNLNSRRAVRPVKPKQPNRTPKRKSPQHLLALCALG
                     FCLFILGLVHVWLRLQVVHMGYVLSTTTKLQGRLEQENRELKVELASLTSPDRLEAMA
                     RRRLGLMPPEKGQVIVLP"
     gene            complement(1888..2817)
                     /gene="rsmH"
                     /locus_tag="VF089_21420"
     CDS             complement(1888..2817)
                     /gene="rsmH"
                     /locus_tag="VF089_21420"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6176577.1"
                     /translation="MDFAHTSVLIQESMGLLKPRPGERYLDGTLGGGGHAEEILLRSN
                     PDGRVLGLDWDEDAVRAAAERLSRFGDRLTLRRANFVQAKEILADINWNQVNGVLLDL
                     GVSSHQFDAAERGFSFQASAPLDMRMDRRQPLTALEIVNSFSESQIAKIISDYGEEPR
                     ARRIAAAIARERKNRAIETTIDLADLIVRVKGKRNRAHHPATQTFQALRIAVNRELET
                     LKTFLEYGYELLQPGGRMVIISFHSLEDRLVKTAFRKWSEKCICPPRTLHCECGWSQK
                     ARRLTRKPIIASAEEVRANPRARAAKLRAVVRL"
     gene            complement(2817..3179)
                     /locus_tag="VF089_21425"
     CDS             complement(2817..3179)
                     /locus_tag="VF089_21425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6176578.1"
                     /translation="MLPQNNIQDISVIDVSGDIDFREMIRIKNVIGGLIEKDRLKVVL
                     NLRAVDHINYLSIGVLLERLKLLRNLNGDLKLAGMSPFLRDIFKVVGMDRFFEDYTSL
                     EDAIESFDDDWEGDGTSH"
     gene            complement(3228..3680)
                     /gene="mraZ"
                     /locus_tag="VF089_21430"
     CDS             complement(3228..3680)
                     /gene="mraZ"
                     /locus_tag="VF089_21430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6176579.1"
                     /translation="MFRGSFEHTLDGKGRLSIPSKFRDILLGKGDERIIMTNFVVDGL
                     RCLDVYPIDEWLRLEEQISQRPKFDRRMVLFQNFYLGGACECLLDKQGRILIPTSLRK
                     YADLKRDVVLVSALEKFRVWDQETWKQFFADAEGKLMQDPDFLGELGL"
     gene            complement(4001..4368)
                     /gene="rnpB"
                     /locus_tag="VF089_21435"
     ncRNA           complement(4001..4368)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF089_21435"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4724..5452)
                     /locus_tag="VF089_21440"
     CDS             complement(4724..5452)
                     /locus_tag="VF089_21440"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX6176580.1"
                     /translation="MRAQIEILASLQIIDREIREHAAVKQGLLGELQEKQRLIQERKQ
                     DIEVLTAGCAAKEKLRLEKDKLLHDESQKATDKRMRMNRIKNVKELQALQREIDQIRQ
                     ANGEAEEELLNLMVEIDGLKGEIKGKQDSLVAAQQDWQQKQEALQAQIAAIDEAVDKA
                     SIRRQRIAQEIDANLMSRYELIFSRRGGSAVVEVAGGICQGCYMNIPPQLWNEIIRSE
                     KLNLCPSCQRILHYKAPEGQENTA"
     gene            complement(5661..7043)
                     /locus_tag="VF089_21445"
     CDS             complement(5661..7043)
                     /locus_tag="VF089_21445"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6176581.1"
                     /translation="MKTLVERLASITSAQWVTTDPALLDTLSWDALSEGRVHPLRSFD
                     TNRPACAVFPGSVDEVRRIVLLANEQKIPIIPYGGGSGLMGAALSVQPGIVVDLRRMN
                     RPLEIDIAARSARVQAGIVLEALEQRLNEQNFILGHDPWTLPVATVGGAISTNSVGYR
                     AGVYGSMGEQVLGLEAVMANGEILRKRAVPKSSTGPDLRLLLIGGEGCFGIVTEATLR
                     IFPKPEKRVVRGLLFESFESGFDAIQKLFAACLRPALLDFGDDDEKNTQGAVLYIGFE
                     GAVEIVTAEDRLATDILSRNGGKHLPKLEAETFWENRHAIARRFLASRSQRRERGRDG
                     IYRDWIHVALPASNVLAFRRAAMVIIEQHGVDLHESGLWIQPELFSMRLGIDDHRKNK
                     AQLALQETVDNLLGLAQDMGGSMEYTHGVGVKLAPFMLKEHGYGLEVMRSLKKTLDPN
                     NIMNPAKMSL"
     gene            7798..8664
                     /locus_tag="VF089_21450"
     CDS             7798..8664
                     /locus_tag="VF089_21450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6176582.1"
                     /translation="MLAILLALFGSFWFAVSMIFINRGVLAVDYFKGLLANLGVNALC
                     LCVYLSLSADRIDLWAPANLLFVVVGIFVPGVSRFFIFKGMERLGAPISACLTNSTPL
                     FAIVFAVFFLGERPTLTNLLGAVSIVAGIISLSWRGAAKTWRTRDLIFPLTAAFLFAG
                     RDNLVRIGVLQIGSPIMGAAIASVVSLATMALLYVVFAEHKPLGPLGLRGLALFGVSG
                     FMNFLSYVFIYTALSMERVSLISPLVNASSLFVVPLSLIFLKDVEKLTARKFGATALV
                     ILGVLLISWEKL"
     gene            complement(8816..10165)
                     /locus_tag="VF089_21455"
     CDS             complement(8816..10165)
                     /locus_tag="VF089_21455"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6176583.1"
                     /translation="MKRTTSRFPNLLDRIYPAEQRRRSAAKAVLLGALATVYFLLPPP
                     ALESLKKEEDIALVSSSQIVHHPDPLYRPFEEKSATVSSSETVDLTLRRGDTLQGTLL
                     RYGLTPASANDLIKQLQPFYDPRKMRAGDTFRLLLDHENEIQGLEHSADGAIVRVTST
                     ENGWAAKRHEVPFVRESKVIRGNIRGSLFEDGVDAGLSPPQIHKLVNLFEYEIDFFSD
                     LRRGDDFSVIFEEKQYANGHREQGKLLAAEIQAGGSPYQIFYYQGKRQKGSYYDNRGE
                     ALMKAFLRAPLNYRRISSPFRINRRHPIFRTVRPHQAIDYAAPTGTPVVAIGHGQVTF
                     SGVRGGYGRMVEIRHNDGYVSRYAHFSRIPKAIRVGKSISRGEVVGYVGQTGHATGPH
                     LHFEMLKKGRKVNFLDLKLASEERLTGSELQEFLALREKKLALLQGSVAVSPENPHS"
     gene            complement(10261..10614)
                     /locus_tag="VF089_21460"
     CDS             complement(10261..10614)
                     /locus_tag="VF089_21460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6176584.1"
                     /translation="MVPSIGASAGVYGILLAYGLIYPNQIIYFYFLFPIKMKHFVLII
                     GAIALYSSISSGQSGIAHIAHLGGMLFGYFYLRGGNPFEGFKLYLAQRRLARMKRRFR
                     VLEGGKDDDSKPTLH"
BASE COUNT         2451 a         3024 c         2688 g         2560 t
ORIGIN      
        1 gcgacgcgct ttttggcatc atagccgcca tttaccagat ctaccttttg cgcagtgcca
       61 gtcttaccgg caacattaaa cccttccact ttcgccattg ttccggtgcc gccctcggta
      121 gtaacgtctt gcagcatgga ggccagcgaa ctggcggtcc tctctgagat cacacgacga
      181 accacatgag gctgattttc caccatgact tcgccctttg gaccgacagc ccgccgaacc
      241 acatagggac gcatgagaaa gccgccgttg gcgatcgccg cataggccat caccatttgc
      301 agcggcgtcg cggagagtcc ctgcccgaat gcatgggttg ccaaatctat ccctgaccag
      361 gtttccggcc gccgcagtaa tcctggaact tccccgggca tgtcgatgcc cgtcacttga
      421 ccgaaaccaa atttctcgat gtacttgaag aatcgatctt ttttcaactt gtcggcaact
      481 ttagagaagc cgatgttgct cgatacctga aggatcttcg agaacggcag ccagccatgc
      541 ggatgggtat cgtgaatgat tctcccggcg taactaaatt taccgtattc acagaaaaac
      601 aaatcctctt tgtcgacgat tccctcttcg agagcggcgg ctgcgaggat tgttttgaac
      661 gtggaacccg gctcgaaact gtcggtcacc gcacgattgc gccgctgctc aggtgaggcg
      721 gccgaataat tgtttggatt gaacgtgggg tagttggcca gtgccaaaat ttcccctgtg
      781 cgcggttcga ccgcaatcgc aacgcctgcc tttgcgcggt atttatcgat ggaagcttcc
      841 agctctttct ccgcaagatg ctgaatggaa gtgtcgagcg ttagatgaat atcgccaccg
      901 gcaggaattt gcaggccctc gacgccttcc acgaggacac gtcggcccaa agcatcacgc
      961 tcagaaacag aggagccggt ttctccacga atatattcgt tgtagcgcag ttccaaacct
     1021 tcaagccctt gcgaatcatg gccgacaaaa ccgatcacct gcccggcaag ctgaccctgt
     1081 gggtagtaac gatgcggttc gtgaaacatg cctacgcctt cgattttgag attctgaatt
     1141 tgacccgcct catcggggga aatctgccgt ttgatccaga cgaaaggctt ggcagaggtc
     1201 agcctctctt tgatctccgc tgtactgatc tttagaactc gcgctaaatc acgagcgctc
     1261 ttggaagcat cctcaatgcg ccgggggcgc gcgtaaaccg actgtgcctc gagactcagt
     1321 gccagcgctt cgccaccccg atcgaatact ccgcctcgct tcggcagcac ggtccattcc
     1381 ttcagatgtt gccgctgccc caagcgcatg agcctatcgc cttgcacggc ttgcaaataa
     1441 aatgcgcgcc cgccgatcac caaaaagatc gccagaaaaa gaatcttagc gacagctaaa
     1501 cgaacatggc tggtctgcag cgttcgtctc atggcagtac gataacctga cccttctccg
     1561 gaggcatcaa gcccaatctt cggcgtgcca tcgcttccaa gcgatccggg gaggtcaagc
     1621 tggccagttc caccttcaac tcccggttct cttgttcaag acggccctgc agtttcgtgg
     1681 tagttgaaag cacataaccc atatgaacga cctgtaaacg cagccagaca tgaaccaatc
     1741 ctaaaatgaa caagcagaag cccagagcgc agagagcaag aaggtgctgc gggcttttgc
     1801 gcttgggagt ccggttaggc tgttttggtt tgaccggtct aaccgcgcgg cgtgagttta
     1861 gattaacggc agtggctgtt tttgttctca tagtcgcact accgcgcgca gcttcgccgc
     1921 acgcgcccta gggttagccc tgacttcttc agccgatgcg atgatcggct tgcgggttaa
     1981 tcttcgtgcc ttctgactcc agccgcactc gcaatggaga gttctgggcg ggcaaataca
     2041 tttctcgctc catttgcgaa aggctgtttt gaccagtcga tcttccagtg aatgaaacga
     2101 gatgatcacc atacgcccgc caggctggag caattcatag ccgtattcca aaaaagtttt
     2161 gagagtttcc agttcgcggt tcaccgcaat tctgagcgcc tgaaaagtct gcgttgccgg
     2221 atgatgagcc cgattgcgct ttcctttcac tcgaacgatg aggtccgcga gatcgattgt
     2281 ggtctcgatc gcgcgatttt ttcgctctct ggcgattgca gcggcgatgc ggcgcgctct
     2341 cggctcttcg ccatagtcgc tgatgatttt ggctatctgc gactcggaaa aagagttgac
     2401 gatttcgagc gccgtcaacg gctggcgacg gtccatgcgc atgtccaatg gagcgcttgc
     2461 ttggaagcta aaaccccttt ccgcagcatc gaactgatgc gatgaaacac ctaaatccaa
     2521 gagcacacca ttcacctgat tccagtttat atccgctaga atctccttcg cctgaacaaa
     2581 atttgcccgg cgaagtgtca gacgatctcc gaagcgactc aatcgctccg ctgccgccct
     2641 cactgcatcc tcatcccaat cgagccctaa gactcggcca tcgggattgc ttcggagaag
     2701 aatctcctct gcgtgcccac ctccccccaa agttccatca aggtaacgct ctcctggtct
     2761 gggcttgagc agccccatcg attcctgaat cagaaccgaa gtgtgggcaa agtccattaa
     2821 tgactagtgc cgtccccctc ccagtcgtca tcgaagctct cgatcgcgtc ttcaagagag
     2881 gtataatctt cgaagaaacg gtccatgccg acaactttga agatatctcg gagaaacgga
     2941 ctcatgcccg caagcttaag atcgccgttg agatttctca gcagcttcaa gcgctcgagc
     3001 aacacgccga tacttaaata attgatgtga tcgactgccc ggaggttaag aaccaccttc
     3061 aagcggtctt tttcgatcaa tccgccgata acgtttttaa tgcggatcat ctcgcgaaag
     3121 tcgatatcgc ccgaaacgtc aatcacggaa atatcttgaa tattattttg cggtagcatt
     3181 cttattctcc tttcatccag ccacggcgaa atccgccccc gccattctca caaccccagc
     3241 tcgccgagaa agtcaggatc ttgcatcaat ttgccttccg catccgcgaa gaactgcttc
     3301 caagtctctt ggtcccagac acggaacttt tcgagcgccg aaaccagaac aacgtcgcgc
     3361 ttgaggtcgg cgtatttcct caacgaggta ggaatgagaa tacgtccctg tttatctagc
     3421 aggcactcac acgcgccacc caagtaaaag ttttgaaaca acaccatacg gcggtcgaac
     3481 tttggtcgct ggctaatctg ctcttcgagc cgtagccatt cgtcgatggg ataaacatca
     3541 agacagcgca agccgtcgac gacgaagttt gtcataatga tccgctcatc gccttttccg
     3601 agcaaaatgt cgcggaattt cgacgggata cttaaccgtc ccttgccgtc aagagtgtgt
     3661 tcaaagctgc cgcgaaacat ataagaagtc ggctaaaaga ctggcttatg ggaaattatc
     3721 ccactttctc ccaccactgc gccacttata acttcacgag tacccactgt caataaattt
     3781 gttcaacttt tcccgtcttt ttcgtctttt atagcttcgg tcgaactaac ggagtggcgg
     3841 gttataacag cgcaagaaga tagtatcgtg cgcttaagaa tttgggagag gtttggcaga
     3901 cctgaagtgg gatcgcagcc caaatttagg cgaagctagc cagggaaaga aaaggagtga
     3961 gacaacggaa acggaggagc aaatgcaagg atgaaactga agctaaagtg gatcgataag
     4021 ccggattctg taatgactct caaaaagagt cacagcgatc attcatcttg ccccaggatt
     4081 gctcctgggt tcgagcgacc ttacccgagg gctatgggcg gaccacccaa ccctcctatt
     4141 tggtcttgct ccaggtgggg tttgccatgc gtccatcatc actgatgaac cggtgagctc
     4201 ttacctcgcc ttttcaacct tacctgccaa aagacaggcg gtatattttc tgtggtactt
     4261 tcccccgatc actcggagcc gccgttaacg gccaccctgt cctgtggagt ccggactttc
     4321 ctctcctcgg attacagaat cgtgagaagc gatcgcttga tccactttag cccgttagtt
     4381 ttttggagcc tcatcgacgc ctcgctgagc gagccaatcg gctaccttct tcgatttacg
     4441 tatacatgaa ctatgcgata gggtcgagcg acaaatttgt agccttgagg gcctgctctt
     4501 catctttgac ctggtagcac ccgaacgcta gaaggtgcga tcttaggttc ttacttggca
     4561 ttcactcgca caccgcttcg aaaccaagct cgcagcacgg ccgcgcttca acatcaaccg
     4621 aggcccaagc acttatatat ttcaagttgt aggagccgga ctgatttgaa ccatgttatc
     4681 tgtaccggca gagggtaaat cgagaagccg aaacttaaga ttattaggcc gtgttttctt
     4741 gcccttccgg agccttgtag tgcaggatgc gctggcagct aggacaaaga ttaagctttt
     4801 cgctacggat gatctcgttc cagagctgcg gcgggatgtt catataacaa ccttggcaaa
     4861 tgccgcccgc aacttcgaca accgcactac cgccgcgacg tgaaaatatc aactcatagc
     4921 gggacattag attggcgtcg atttcttggg caatgcgctg ccggcggatt gaggctttgt
     4981 ccaccgcctc gtcaatagcg gcaatctgtg cttgcaacgc ttcctgcttt tgctgccaat
     5041 cctgctgagc tgcaacgagc gaatcctgtt tacctttgat ctctcccttt aggccatcga
     5101 tctctaccat caggtttagc aattcttctt ctgcctcacc gtttgcctgc cggatttgat
     5161 cgatctcccg ctgtaaagct tgcagctctt tgacattctt gatgcgattc atccgcattc
     5221 gcttatcggt tgccttttgg ctttcgtcgt gcagcaattt atctttctcc agacgaagct
     5281 tctccttcgc cgcgcagccg gccgtcagca cttcaatatc ctgttttctc tcttggatca
     5341 gtcgctgttt ttcctggagt tcgcccaaca ggccttgttt gacggcagca tgttctctta
     5401 tttctcgatc tataatctgt aatgaagcca gaatctcaat ttgtgcgcgc aacttcttct
     5461 ccctcagtca acaaaaaatg cacccggatg ggtgcatttg tttaaaagcg tttgtctata
     5521 atccttacgt tggaagtagg tgactctcac tcctgagctc caccacaata tttatatttc
     5581 ttagttaaca ttgtcaaagc aagtctagcg ctgcgtgtca gggtcggctc catgcgccgg
     5641 gacagcttga tgcgctcaaa ctaaagactc atctttgcgg gattcatgat gttattggga
     5701 tcgagcgtct ttttgaggct gcgcatgact tctaatccgt agccgtgctc ttttaacatg
     5761 aagggcgcaa gtttgacgcc cacgccatga gtgtactcca tggaaccgcc catgtcttgt
     5821 gctagcccga gcaggttatc gactgtttct tgcaacgcca gctgcgcctt attttttcgg
     5881 tgatcgtcaa tgccgaggcg cattgaaaac agctccggct gaatccagag cccgctctca
     5941 tgcaggtcca ctccgtgctg ctcaatgatt accatggccg cccgtctgaa cgctagcacg
     6001 tttgaggcgg gaagcgccac atggatccag tcccgataaa tgccatctcg cccacgctct
     6061 cgccgctgcg agcgactggc caaaaagcgc cgggctatgg catgccggtt ctcccaaaac
     6121 gtttccgcct caagtttcgg cagatgtttg ccgccgtttc gcgataagat atccgtagct
     6181 aatctgtctt ccgctgtaac gatctcgaca gctccctcga aacctatata aagcactgct
     6241 ccttgcgtat ttttttcatc atcatcgccg aagtccagca gcgccgggcg caagcatgcg
     6301 gcgaagagtt tttggatagc atcaaacccc gattcgaacg attcgaaaag caacccccga
     6361 acaacccgct tctccggctt tggaaagatc ctcaacgttg cctctgtgac aataccaaag
     6421 caaccctctc cgccgatcaa gagaagacgc agatccggac cggtggaaga tttgggcacc
     6481 gcccgcttgc gcaaaatttc cccgttggcc atgacagctt ccaggcctaa aacctgctcg
     6541 cccatcgaac cataaacgcc tgctctgtat ccgacgctgt tggtcgagat ggcgccacct
     6601 acagtggcaa ccggcaaggt ccacggatca tggccgagaa tgaaattctg ctcgttcaat
     6661 cgttgctcca acgcctcgag cacgattccc gcttggactc gtgcggaacg cgctgcgatg
     6721 tcaatttcaa gaggtcggtt cattcttcgt aaatcgacga caattccagg ctgaactgat
     6781 agagcagcgc ccatgagccc cgagccgccg ccgtatggaa tgataggtat cttctgttca
     6841 ttggccaaca gaactatccg cctgacttca tccaccgaac cgggaaaaac tgcacaggcg
     6901 ggccggttcg tatcgaacga tctgagcgga tggacgcgcc cttcactcaa agcatcccag
     6961 gataaggtat ccagaagtgc cggatcggtt gtaacccatt gagcagaggt gatcgaggcg
     7021 agcctttcta ccagggtctt catcgtttta gctgccgttc gctaaatata gcccgatcga
     7081 tgatccgcgg aaagcgccac caaataagcg gccgtattcg gaaacatggg aagacgtcaa
     7141 ccggcgggga aattctcgaa actaataaag agcccaaact ccctcgaata cgcacgtctc
     7201 aacatctgct ggacaaatcg ccgcagcgtg ttcaagagcg aatttgtgag taccacctag
     7261 gtgatcgagc attccgacac ggctgataaa gccgacaccg tgctcgagac agaagaagga
     7321 cgcattcaca accgaaggcg cgggcaaata gtacggcgaa tggtagtagc caggcgggcg
     7381 cacgacgatg atgcgcgccg ggcgcctacg gtgaaatcca gggatgcgat gcgaatgtct
     7441 ctcacttgaa tcttctctgc ggttcaagcc ggcaacgctc ggcgcctgcc gcgaaacgtg
     7501 agtatcggat cggcgcgacc cgcgagcgcc cgaggccaac gccgatggag cagaagccgc
     7561 taagaggaca acgaccagaa aaactatgct ggttctaaca gtcgtgtaac cgccaaagaa
     7621 gcgcatgaat cattgtagca gaccaccagc tgatttcatc tctccgtgaa cgggctagac
     7681 gatcttttct atctctagcg aacgcgccct tgaagccgcc gaccagccca ccagacgagt
     7741 tttgtgactc accattgaac atgaaaggcg ctgcaggtaa ggtccccgat taatttcatg
     7801 ctagcaatcc ttttagccct gttcggctca ttctggtttg ccgtttcgat gatctttatc
     7861 aatcgcggcg tcctggcggt cgattatttc aaaggactgc tggccaacct tggagtaaac
     7921 gccctatgtc tctgcgtcta tctgtcactg tccgccgatc ggatcgatct ctgggccccg
     7981 gccaatctcc tattcgttgt tgtcggaatt ttcgttcccg gcgtatcccg tttttttatt
     8041 ttcaaaggaa tggagcgact cggcgcgccc atttcggcat gcctcacaaa cagcacgcct
     8101 ctttttgcga tcgtctttgc cgtcttcttt cttggcgagc gcccgacgct taccaacctg
     8161 ttaggtgcgg tttcgatcgt tgccgggatc atttcgctct cctggcgcgg cgccgccaaa
     8221 acatggcgca ctagggatct catttttcca ttaaccgccg cttttctttt tgccggacgg
     8281 gacaacctag tccgcatcgg cgttctccag attggttcgc caatcatggg ggcggcgatt
     8341 gcgtcggtgg tatcgctggc aacgatggcg ctgctctacg tggtctttgc cgagcacaaa
     8401 ccgctaggac cgctcgggct gcgcggccta gctctctttg gcgtctcggg attcatgaat
     8461 ttcctttcat acgtattcat ctacaccgcg ctcagcatgg aacgagtatc gctcatctcg
     8521 ccactggtaa acgcctcgtc actgttcgtc gtgccgctct cgttaatttt tctgaaagac
     8581 gtcgagaagc tgaccgcgcg caagttcggc gcgacggcac tggtcatact gggagtgttg
     8641 ctgatttcct gggagaagct ctagaaagga agctgccggg atcgtgtcgc gagcaactgt
     8701 tgcgccccgc aaaccatgtc aacaggcggg gcaaacaaga taccgctcat tgctggcggc
     8761 atagactgca gagcgggttt acagagaaca acagcagtca ctcggatgct gctaattaag
     8821 aatgagggtt ttcaggggag acggctacac tgccttgaag caaagctaat ttcttctcac
     8881 gcagggcgag aaattcctgc aactccgatc cggtgagccg ttcctccgat gcgagtttca
     8941 ggtccagaaa atttaccttc cgaccttttt ttagcatttc aaagtgcagg tgcggaccgg
     9001 tggcatgccc ggtctgaccc acatagccga ccacctcacc ccggctgatc gacttaccca
     9061 cccgaatcgc tttaggaatt ctcgagaaat gcgcatagcg ggagacataa ccgtcattgt
     9121 ggcgaatctc caccatacgg ccgtaaccgc cccgaacgcc tgaaaaagtc acttgcccgt
     9181 gtccaatggc aaccacaggt gtgccggtgg gtgcggcata atcgattgct tggtgcggcc
     9241 gtacggtccg aaaaatcgga tgccgtcgat taatccggaa gggcgatgag atccgccgat
     9301 agtttagcgg cgcgcgcagg aatgctttca tcaaagcctc gccgcggttg tcgtagtagg
     9361 aacctttctg tctcttgcct tgataataaa aaatctggta cggcgatccg cccgcctgaa
     9421 tctctgcggc gagaagctta ccttgctcgc ggtgaccatt ggcgtactgc ttttcttcga
     9481 aaatcactga aaagtcgtcg ccgcgccgga ggtccgaaaa aaaatcgatc tcgtattcaa
     9541 ataggttgac taacttatgg atctgcggcg gcgaaagtcc cgcgtcaact ccgtcttcaa
     9601 acagactgcc gcggatgttg ccgcgaatca ctttactttc gcgtacaaat ggaacttcgt
     9661 gtcgcttggc ggcccaaccg ttctcagtcg acgtgacgcg aacaatcgcg ccatcagcgg
     9721 aatgctccag accctgaatt tcgttttcgt ggtcaagcag caaacgaaag gtgtcgcccg
     9781 cgcgcatttt tctgggatca tagaaaggtt gcagctgctt gatcaagtca ttcgctgaag
     9841 cgggtgtgag cccgtatctc agcaaagtgc cttgcaaagt atcgccgcgg cggagcgtca
     9901 aatcgacggt ttcggatgac gacacggtgg cgcttttctc ctcgaaagga cgataaagcg
     9961 gatccgggtg gtggacgatt tgcgaggagc tgacaagcgc aatatcctct tcctttttca
    10021 atgattccag cgccggtgga ggcagtagaa agtatacggt tgccagcgcg ccgagaagca
    10081 cagccttggc ggccgaccgg cgcctctgct cggccggata gatccggtct agcaaattag
    10141 gaaaacgaga cgtggttctt ttcatgccgt taactcgccg aagataattt tcccttttta
    10201 caacatcttg atcaattatc aaaaccagaa ggcaagaatt tttgctaata gccgtccaga
    10261 tcaatgcaaa gtcggttttg aatcatcgtc tttgccgcct tcgagcacgc ggaagcgtcg
    10321 tttcattcgg gccagtcgtc gctgcgccag gtaaagcttg aaaccctcga aaggattccc
    10381 accacgaaga tagaagtaac cgaacagcat gccgcccaaa tgagcgatgt gggcgatgcc
    10441 gctctgcccc gagctgattg acgaataaag cgcaatcgcc ccgataatca acacgaaatg
    10501 tttcatcttg atcggaaaga ggaagtaaaa atagatgatc tggttcggat aaattaggcc
    10561 gtaggcgagc aagattccat agactcctgc cgaagctccg atgctgggaa ccatttgatg
    10621 tggagcaccc gctcttcgtg gccaacgagg gcaagctctg cgccgtcgtc cgcgaggagg
    10681 acgcgaaggc cgccctcgat gcctggcggg cgcacccgct cgg
//
LOCUS       DASUTG010000924         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1035009, whole genome shotgun sequence.
ACCESSION   DASUTG010000924 DASUTG010000000
VERSION     DASUTG010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035009"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF089_21465"
     CDS             complement(<1..635)
                     /locus_tag="VF089_21465"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176585.1"
                     /translation="MFRVISYLAFMLLLGIVSPSLGSAQERVRVGWAAMTASHTPLWV
                     AQEKGFSAKQGLAVESIFFGAGPPAMQALVAGDLDIVITSAPNVVNPRLGGADTVMIL
                     SIIPTFIDHIISAPNITTTEQLRGKIGSVNRFGSISDMGLRLSLRRLGIDPEKDVRIV
                     PAGGNPERLAAISKNISQFTIMNEPFIKEAERLGYRDLVNMATLKIPLHGNG"
     gene            complement(920..1471)
                     /locus_tag="VF089_21470"
     CDS             complement(920..1471)
                     /locus_tag="VF089_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6176586.1"
                     /translation="MDLRGVISPPETFESERLQLRKPVLADAEEIFRQYAQDAQVTKY
                     LTWLPYGKVEETRAFIRTCLATWQEGNSYHWTLVRKEDSQLLGMINARVDGHKWELGY
                     VLARKYWGRGYMTEAVKSLIDTAFKDGAVYRVWAVCDVDNVASARVMEKAGMQREGLL
                     RRWSIHPTSSAEPRDSFCYSITK"
     gene            complement(2357..3340)
                     /locus_tag="VF089_21475"
     CDS             complement(2357..3340)
                     /locus_tag="VF089_21475"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176587.1"
                     /translation="MLVKSSVIVTTVLFLLYAAPCGSQEKIKLRVSSATKTLGYGPLW
                     IASKMGFFERQGLDVDLVVIRASDVGIQALAGGSLEIAGSSADAPIAAVEKGLDLVMI
                     GGIINGLTQSIMAAKKYKTYADLRGATFGAISLTSGVTFALRQVLKVKGLEYPRDYKL
                     LVIGGTPQTYAALTAGQIDAAALSLPVNYAAEEQGFNEIGKFVDVIPNYQLAALSLKR
                     SWGEKNRPVVVRFSKAMAQTMRWIYQNKQPAVDFLAKEMNLKPDHARRGWEFYTGSRM
                     WHPDGDINLEGLQVVTQIYAEQTQAKGPVANAVKYIDQSYLKEALKELGSQ"
     gene            complement(3376..4587)
                     /locus_tag="VF089_21480"
     CDS             complement(3376..4587)
                     /locus_tag="VF089_21480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176588.1"
                     /translation="MSANRILEGVRILTFTTGYAGPYAGRLLASYGAEVIKIESRKGG
                     LDTFRHYGQHKDIDAAPRFIECNLGVRSLSVNLKHPIGQRLIKELAGKADAILQNFRP
                     RVLDKFGLGDEELRKVNPKLAILKMPGFGSYGPKSSYGTWGFNLTAFSGMTYLWNHPE
                     QDRPIGSQGVYPDHLGFILGPTLMVAALLNSRRTGKGVTIDLAQIEGTAYTLGTTYLE
                     ASVNGDDPKPQGNHYGVAAPHGCYRCRGDDRWCVISVQNDDEWRSFCRVVGAPELTGD
                     SRFADRQARLRHSSELDAVVQQWTETRSAEDVMTKLQSAGVAAGVVQTGADLLNDPQL
                     RHRNYFAAFPDSLIGAFEVPRGGILFKGMEEEPFRLPNRFGSDNDQILGEILGYNKVT
                     IDQWRGEDVLT"
     gene            complement(4690..5898)
                     /locus_tag="VF089_21485"
     CDS             complement(4690..5898)
                     /locus_tag="VF089_21485"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176589.1"
                     /translation="MNNTTNSAALLPGFRVLDLTSSMGALCGKLLSDMGMDVIKIEPP
                     QGDSARCEPPFAKGHAHREASLRFVYLNAGKRSITLDVTKPQGKRLLLRLVSSADIVL
                     ETFAPDFLASQNLGYSDLVEIQNNLILVSLTGFGQDGPYAHFKTPDIVGTAMGGLLYI
                     SGDPKMTPCNPPETQSYYYASLFAAYGVTLALWQRETRGIGAYIDASIQASMALHEHV
                     AFNYSAEGRVMKRAGSQHQHNAPANLFKCRNGYIALFVTQTHWPLLLKVWEDHPAELD
                     DPKWINSNLRRANADYINAQVTSFTSRFLKEDLAELMQKHGIPGLPVNTPSDFMKDPH
                     IRTRGFFDTVTHPVLGSFEQSGSPFMVDGRRAAPSPAPVLGQHNYEVFCGELGLEQSE
                     LEVMAADGVI"
     gene            complement(6046..>6509)
                     /locus_tag="VF089_21490"
     CDS             complement(6046..>6509)
                     /locus_tag="VF089_21490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX6176590.1"
                     /translation="FAIAQGWIQEGRIRGHGARPVGIASRALDMMIEYSKVRTTFGRP
                     LADRQAIQFMIADSAMELHACRLMVYECAWRHDRGEDVRQLSYMTKIMCAEMASRVVD
                     RSIQVHGGLGLMRDLPLEWWYRQVRSIRITEGTPEVLRWRLAQHIIKNHKS"
BASE COUNT         1457 a         1809 c         1736 g         1507 t
ORIGIN      
        1 ccgttgccgt gcagcgggat cttcaacgtt gccatgttaa ctaaatcgcg atagccgagg
       61 cgttcggctt ctttgatgaa aggctcgttc atgattgtaa actgcgagat gtttttcgaa
      121 atggccgcga gccgctcggg attgccgcca gcggggacaa tcctcacatc cttctccgga
      181 tcgatgccta accggcgcag tgaaaggcgc agccccatgt ccgaaatact gccgaatcga
      241 ttgaccgaac cgatcttccc gcgcaactgc tcagtggtcg taatattcgg tgcgctgatt
      301 atgtggtcga tgaaagtcgg gatgatcgac agtatcatca cggtgtcggc gccgccgagt
      361 cggggattaa ccacgttagg cgccgatgtg atgacgatat cgagatcacc ggcgacgagc
      421 gcctgcattg caggtggacc ggcgccaaaa aaaatcgact ccaccgccaa accctgcttg
      481 gctgaaaatc ccttttcctg cgccacccaa agcggggtgt gcgaagcggt catcgccgcc
      541 cagccaactc gcacccgctc ctgagccgag ccgagagacg gagaaacgat acctagcaaa
      601 agcatgaatg ctaaatagga aataactctg aacatcaact tcacctcaag aagccactag
      661 gccttatatt aattccagcg caatcatatc gcttcgcctg ccatcctaat tgacggtttt
      721 tacaataaca aggaatcgtc taacctcgcg actactttta gtctgaagaa aactctgctt
      781 acaggctgct caaaaagact cagaggcgag gcgcgcgaag ttcgatgagc tgaggcgtac
      841 acttcagcac gttgaggcga gtcgggcgag cgtaacgaag cctatgagtt ttttttcagc
      901 agcctgctag gctaggaatt tacttggtga tggagtagca aaatgaatcg cgcggctcgg
      961 cgctgctggt cggatgaatc gaccaccggc gcagcagtcc ttctcgctgc atgccggcct
     1021 tttccatcac ccgcgccgat gcgacgttat cgacatcaca cacagcccag acgcggtaaa
     1081 ccgccccgtc tttgaaagcg gtatcgatga gcgacttgac cgcctcggtc atgtatcccc
     1141 taccccagta tttccgggcc aggacatacc cgagttccca cttgtgaccg tctactctgg
     1201 cgttgatcat gccgaggagc tggctgtcct ccttgcgcac cagggtccaa tgataggaat
     1261 tgccttcctg ccaagtcgcc aggcacgtcc ggatgaacgc gcgagtctcc tccactttgc
     1321 cgtagggcag ccaggttaaa tacttggtga cttgggcatc ctgggcatat tgccggaaga
     1381 tctcctcggc gtcggccaag accggttttc gcagttgcag tcgttcgctc tcgaacgttt
     1441 caggcgggct aatcactccg cgcaggtcca ccaggcataa tcgtgaagtt taggttcgct
     1501 tcgaagaata ttcaagagtt attcatcccg catcgctggc gaatacacgt tctctaatcg
     1561 ggatcagccg ccaaggccat cacggtctga ttccgcgcgg gtacgataaa actttcaagc
     1621 atggcaggca cgcaaataca tgatcatccg gcatgccgca gacttcgtcg ctttccattg
     1681 tcattctccg gcatagcggg caacgatgct ggtgttttct aaccgcggaa tatgacgtat
     1741 tgagttttgc ggcgccacgg agcgggatta ttctagcctt catttctacg atgtctctca
     1801 atatagcgcc gatcccattg gtcatggcgc caatgcgatt attcagagga gcaacgtcaa
     1861 tgccaagcta gcagaggatt ttgacagcga caaacgctta gttagcgccg gattttcgga
     1921 gaagtgcgta attttatgag tgaagcggga tggagaaatg caactttgga tacatcgtgt
     1981 atccgaagtt gttattggct tgatttagca gagatggtca gagaactcgt tcgaattatc
     2041 cttgatcttc aacatcggct caccgatgta tctgcgaaac aacgggactt gccgctgctg
     2101 gcatgtccga tgtgatgtac tttgtcctgg tatgaacaaa cgtggcggca aggtttagcg
     2161 gagtcataat tggttaatat agtcggccga agccaaactc ccggcaccag gcatgttgtc
     2221 cctactgtat taaaattacc ggcatggact tgccgatgta gtgcgccatc agcgggtgta
     2281 aaaatcgtag cgacgatcaa cgattgatag ccctagccat gaatttgttc atccggttct
     2341 gtcgtccggc aaaccgctac tggctcccaa gctctttgag cgcttcttta agataactct
     2401 gatcgatata tttgaccgcg ttcgctaccg gacctttggc ttgggtttgt tcggcgtaaa
     2461 tttgggtgac cacctggagg ccttccaggt tgatatcgcc gtcgggatgc cacatgcggc
     2521 tgcccgtata aaactcccat ccgcgcctgg catgatcggg tttgaggttc atttctttgg
     2581 caaggaaatc caccgctggt tgtttgtttt gatagatcca gcgcatggtc tgagccatgg
     2641 ccttcgagaa gcgcaccaca accggccggt ttttctctcc ccaggagcgc ttcagagaca
     2701 acgccgcaag ctgatagttg ggaatcacat cgacgaattt gccgatttca ttgaagccct
     2761 gctcttccgc ggcgtaattg accggcagcg acagcgccgc ggcgtcgatc tggccggcgg
     2821 tcaaagccgc gtacgtctgc ggcgttccgc cgatgacgag aagcttgtaa tcgcgcggat
     2881 actccagccc tttgaccttc agcacctgcc tgagtgcaaa cgtcacaccg gaagtcagac
     2941 ttatggctcc aaaggtcgcg ccacgcaaat cggcgtaggt tttgtacttc ttagccgcca
     3001 tgatcgactg ggtaagtccg ttgatgatgc cgccaatcat taccagatcg agtccctttt
     3061 caacggcggc aattggcgcg tcggcggagc tcccggcaat ttccagcgaa cctcccgcca
     3121 gcgcctgaat acccacgtcg gacgcgcgaa tgacgaccaa atcgacatcg agtccctggc
     3181 gttcgaagaa acccatcttc gacgcaatcc acagtggacc atagccgagc gtcttggtcg
     3241 ccgagctaac acgaagcttg attttttctt gggagccgca tggtgcagca tacaggagaa
     3301 acagcacagt cgttacgatg acagaggact tcaccaacat gagcgcctcc tttagagagc
     3361 tgttcgcgac ggccgtcaag taagaacatc ttccccgcgc cattgatcga tggtcacttt
     3421 gttgtacccc aggatttcgc cgagaatctg atcattgtcg gaaccgaatc gattgggcaa
     3481 acgaaaaggc tcttcctcca ttcctttgaa tagaatgcca ccgcgcggga cttcaaatgc
     3541 gccgatgaga gaatcgggaa acgccgcgaa gtagtttcga tgcctcagct gcggatcgtt
     3601 cagtaagtcg gcgccagttt gcaccacgcc ggctgcaacg cccgcggatt ggagcttggt
     3661 cattacgtct tccgcgcttc ttgtttccgt ccactgctgc acgacggcat cgagttcgga
     3721 tgaatgcctg agtcgcgcct gacgatcggc aaaccggctg tcgccagtca actccggcgc
     3781 accgacgacg cggcagaaac tgcgccattc atcatcattt tgcactgaga tgacgcacca
     3841 ccgatcatca ccccggcacc gataacagcc atgcggcgcc gccactccat aatgattccc
     3901 ttggggtttt ggatcatcgc cgttaaccga agcttcgaga taggttgtgc cgagcgtata
     3961 agcggtccct tcgatctgcg ccaaatcgat ggtgactcct ttccccgtcc ggcggctgtt
     4021 taacaaggcg gcgaccatca aggtcggtcc gaggatgaat cccaaatgat cgggatacac
     4081 tccctggctg ccgatcgggc ggtcctgttc cgggtgattc cacagatagg tcatgcctga
     4141 gaaggcagtt aaattgaacc cccatgttcc atagctgctc ttgggtccgt aggagccgaa
     4201 gcctggcatt ttaagaatcg caagtttagg attaactttg cgaagctcct catcgcctaa
     4261 accgaattta tccaatactc tgggacggaa gttttgcaaa atcgcatcgg cttttcccgc
     4321 gagctctttg atcagtcgct gcccgatcgg atgcttgaga ttgacgctta gcgaacgaac
     4381 gccgagattg cattcgatga agcgcggcgc cgcgtcgatg tctttgtgtt gtccgtaatg
     4441 gcggaaggtg tcgagaccgc ccttgcggga ttcaattttg attacctcag cgccataact
     4501 ggccaatagc ctgccggcgt agggaccggc atacccggtt gtgaaggtca ggatccgcac
     4561 gccttccaat atgcgattcg cgctcaacac ggttgttccg atctcctaac gtgatgacgt
     4621 aaattcaagc gaacccctct tgatactcgc atatttttac cggtatccgg agtttaactt
     4681 gagatctttt cagatcacgc catcggccgc cataacttcc aattcactct gctctaaacc
     4741 gagctcgccg cagaagactt cataattatg ctggcccaaa accggcgcag gagacggcgc
     4801 ggcgcggcga ccatccacca taaacggact tcccgactgc tcgaaagatc ctagcacagg
     4861 atgtgtcacg gtgtcaaaga acccgcgagt ccggatgtgc gggtccttca taaaatccga
     4921 cggcgtgttt accggcagcc ccggtatgcc gtgcttctgc atcagttcgg cgagatcttc
     4981 tttgaggaag cgggatgtaa acgaagtcac ttgcgcgttg atatagtcag cgttagcacg
     5041 ccgcagattg ctgttgatcc attttggatc gtcgagttcc gccgggtgat cttcccagac
     5101 tttaagcagg agcggccaat gcgtctgcgt gacaaagagt gcgatgtagc cgtttctgca
     5161 tttgaacaga ttggctggcg cgttgtgctg gtgctggctg ccggcgcgct tcatcacccg
     5221 gccttcggcg gaataattga acgccacatg ctcatgcagc gccatactag cttgaatgga
     5281 agcgtcaatg tacgcgccga tgcctcgagt ctcccgttgc catagagcta acgtaacccc
     5341 gtaggcggca aataggctgg cgtagtagta cgattgcgtt tccggcggat tgcagggtgt
     5401 catcttcggg tcgccgctga tgtagagcaa tccacccatg gcggtgccga cgatgtccgg
     5461 cgtcttgaaa tgcgcataag gtccgtcttg gccaaagccc gtaagggaca ctaggatcag
     5521 attgttttgt atttctacca aatcgctata gcccaaattc tgcgacgcca gaaaatccgg
     5581 tgcgaaagtt tcgagcacga tatcggcgct cgacaccagg cgcagcagta gcctcttacc
     5641 ctgcggtttt gtaacatcta atgtaatgct tcgtttaccg gcgttgagat agacgaaacg
     5701 aaggctggct tcgcggtgcg catgaccttt ggcaaagggc ggttcacaac gggccgagtc
     5761 accttgcggg ggttcgattt tgatgacatc catacccatg tcgctaagca atttgccgca
     5821 cagcgcgccc atggacgatg tcagatccag cactcgaaat cccgggagta gcgcggctga
     5881 gttagtcgta ttattcatca atcatctctg aaattttccc cacagactgc tcaaaagatc
     5941 tcaaatgcga ggcgcgcgaa ggtcggtgag cggagacgta catttcagta cgttggagcg
     6001 agtcgatcga gggtaacgaa gcatatgagt cttttcagca gtctgtcagg atttgtgatt
     6061 cttgatgatg tgctgggcca aacgccagcg caaaacttcc ggagttcctt cggtaatgcg
     6121 gatactgcgc acctggcgat accaccactc gagcggcaga tcccgcatca gccctaatcc
     6181 gccatgcact tgaatcgatc ggtcgacgac ccggctggcc atttcggcgc acatgatttt
     6241 ggtcatgtaa gataattgcc ggacatcctc gccgcggtca tgacgccagg cgcactcata
     6301 aaccatcagc cggcaagcgt gcagctccat cgccgaatcg gcgatcataa actgaatcgc
     6361 ctggcggtca gccagtggac ggccgaaggt ggtgcgcact ttggaatact cgatcatcat
     6421 atccagcgcg cggctggcga tgccgacggg gcgcgcgcca tgaccgcgaa tgcgtccttc
     6481 ctgaatccac ccctgggcga tcgcaaacc
//
LOCUS       DASUTG010000925         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1925356, whole genome shotgun sequence.
ACCESSION   DASUTG010000925 DASUTG010000000
VERSION     DASUTG010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925356"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF089_21495"
     CDS             <1..338
                     /locus_tag="VF089_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX6176591.1"
                     /translation="EKIKVYGVTTKKRVPSLPNVPTMDEAGLKDFEVSIWHALYAPKG
                     TPKPIIDTLTKALQVALKDPTVKQRFADVGTEPVSEDRATPEALRKHLKSEIDRWGPI
                     IKKAGVYAD"
     gene            467..922
                     /locus_tag="VF089_21500"
     CDS             467..922
                     /locus_tag="VF089_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX6176592.1"
                     /translation="MIRSTRDFWTGAIYILIGLSAVLIARDYPMGSAVRMGPAYFPTL
                     LGIILMVVGVVALIRSFIVPGSPIGAFAIKGLLLVVFAVSLFGFIVRDAGLIIALPLL
                     VIISALASSQFRWVPTIALAAGLTLFCSLVFIKGLGVPLPLIGPWFGGD"
     gene            954..2453
                     /locus_tag="VF089_21505"
     CDS             954..2453
                     /locus_tag="VF089_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX6176593.1"
                     /translation="MDLVDNISLGFQTAFTLANLFYCLIGVFLGTLIGVLPGLGPTAT
                     IAMLLPLTFALPPISSLIMLAGIYYGSQYGGSTTSILVNLPGEAASVVTTLDGYQMAK
                     QGRAGVALATCAIGSFFAGTVATFLIAAFGPPLAAIALKFGPAEYFSLMTLGLVASIV
                     LAQGSLLHAFGMVVLGLLLGLVGTDVTSGVPRFTFDIPELADGIGFVTVAMGMFGLGE
                     IIRNLEAEAQRSVVVAKITTLMPTRQDFKRMIAPVLRGTAIGSILGILPGSGSILGAF
                     AAYSIEKKVSKNRAQFGKGAIEGVAAPESANNAGAQTSFIPMLTLGIPSNPVMALMVG
                     AMIIQGIQPGPSVITEQPALFWGIVVSMWIGNLFLLILNLPLIGLWVRMIMVPYRLLY
                     PAILVFCAIGVFSLRNSQFDVYMMALFGILGYVFSKLGCEPAPMLLAFILGPLMEEFL
                     RRAMLLSRGDPLVFVQRPISATLLGIAVLAMCAVLLPSFGKTREVAFKE"
BASE COUNT          509 a          723 c          753 g          697 t
ORIGIN      
        1 cggaaaagat caaagtatat ggtgtgacga ccaaaaagcg ggtgccttct ctacccaatg
       61 taccgaccat ggatgaggct gggctaaagg attttgaggt ctcgatctgg catgctctct
      121 acgcgcctaa gggcactccg aaacccatta tcgatacttt aacaaaagct ctccaggttg
      181 cgctaaagga tccgacggtc aaacagcgct tcgctgacgt ggggacggaa cctgtcagtg
      241 aggatcgcgc cacgccggaa gcattgcgca aacaccttaa atccgaaatc gacaggtggg
      301 gaccgatcat caagaaagcc ggcgtatatg cagactagct ggatcggtta aggaacgaaa
      361 aaaggccgga catatgtccg gccttttttt tcttgacata ttgttgtaga aggcggcttg
      421 atcatcgcgc actaggaggc tttttgcgac aacctggcga cttctcatga tccgcagcac
      481 cagagatttt tggaccggcg cgatttacat tttgatcggt ttgagcgcgg ttctgatagc
      541 acgcgactat cctatgggca gcgcggtgag aatgggcccg gcttattttc ctaccctcct
      601 tggaattatc ttgatggttg tcggagtggt cgcactcatt cgctccttta ttgtaccggg
      661 cagccccatc ggcgcttttg ccatcaaggg gcttttactt gtcgtttttg cggtctcctt
      721 attcggcttt atcgttcgag atgcggggtt gatcattgcc ttgccgctcc tggtgattat
      781 tagcgccttg gcgagcagcc aatttcgctg ggtgccgaca attgccttgg cggccggact
      841 cacgctgttt tgctcgctcg tgttcatcaa ggggttgggt gtgccgctgc cacttatcgg
      901 gccgtggttc ggcggcgatt agtgcgctgt cgccgctaaa cagaagtagc gttatggacc
      961 tcgtcgacaa tatctctctc ggttttcaga ccgccttcac tctcgccaac ctattttatt
     1021 gtctcatcgg tgtctttctc ggaaccttga tcggcgtgct accaggtctc gggccgactg
     1081 ccacgattgc catgctgctg cccctgacgt ttgcgttgcc gccgatatcg tcgctgatca
     1141 tgctggcggg tatctattac gggtcccagt acggcggttc gacaacttcg atcctggtta
     1201 atctcccagg cgaagcggca tcggtagtga caactttgga cggctaccaa atggccaagc
     1261 aaggacgggc gggggtcgcg ctggcgacgt gcgcaatcgg ctcgttcttc gccggcaccg
     1321 tggcgacttt tctgatcgcc gcgttcggac cgccgcttgc cgcgattgcg ctcaaatttg
     1381 gtccggcaga atacttctcc ctgatgacct tgggcctcgt tgcatccatt gtgctggctc
     1441 agggatccct gctgcatgct ttcggtatgg tggtgcttgg acttcttctg ggactcgtcg
     1501 gcaccgatgt gacttcgggg gtgccgcgct tcacattcga tatcccggaa ctcgccgatg
     1561 ggatcggctt tgtcaccgtc gccatgggca tgttcggtct cggcgagatc attcgcaatc
     1621 tggaagcgga ggcgcagcgc tccgtggtgg tggcaaagat cacgactttg atgccgacgc
     1681 ggcaagactt taaacgaatg atcgcgccgg ttttgcgcgg cacggcgatc ggttccattt
     1741 tgggaatatt gcccggcagc ggttcgattc tcggcgcgtt tgccgcgtat tcgatcgaaa
     1801 agaaagtctc aaagaaccgc gcgcaatttg gcaagggcgc catcgaaggc gtcgcggcgc
     1861 ctgagtcggc caacaacgca ggcgcgcaaa cttcgtttat tccgatgctg accttgggga
     1921 ttccgtctaa cccggtgatg gcgctcatgg tcggagcgat gatcattcaa ggcattcagc
     1981 cgggaccatc cgtgatcacg gagcagccgg cgctgttctg gggaatcgtc gtttcgatgt
     2041 ggatcggcaa tttgttcctg ctcatcctca atctcccatt aatcgggctc tgggtgcgaa
     2101 tgataatggt gccatatcgc cttctctatc cagccattct agtgttctgc gctatcggtg
     2161 tcttcagcct gaggaactcc caattcgatg tttacatgat ggcgctgttc gggatactcg
     2221 ggtacgtctt ctccaagctc ggttgcgaac cagcgccgat gttgctggca tttattctag
     2281 gtcctttgat ggaagaattt ctccgacgcg ccatgttgct ttcgcgcggc gaccccttgg
     2341 tattcgttca aaggccaatc agcgcgacat tactgggcat agccgtgctg gcgatgtgcg
     2401 ccgtgctcct gccttcattt ggcaagacaa gggaagtggc gttcaaagag taaccgtttg
     2461 tagtttaggt ccgtcttaca gttcagtgac tcattcgctc tgcaccgcag gacgatcaag
     2521 cctcaatccg acatcgccga tgcggtttcc ttttgtcttc atcagggacg accaaaagat
     2581 cggcagtaaa ccgtccagca ggatttctaa tcttcagagt gatgctcctc cttcggccgc
     2641 cgattacgct ggtcatatga tcgcgaaagt tcaagtccag tt
//
LOCUS       DASUTG010000926         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2221287, whole genome shotgun sequence.
ACCESSION   DASUTG010000926 DASUTG010000000
VERSION     DASUTG010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221287"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..234
                     /locus_tag="VF089_21510"
     CDS             67..234
                     /locus_tag="VF089_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176594.1"
                     /translation="MEEGMTIAKVPDSMTLKPEMMRHLIREHEKLGRTLTEEETVTLL
                     RSVGFEIIEGK"
     gene            264..716
                     /locus_tag="VF089_21515"
     CDS             264..716
                     /locus_tag="VF089_21515"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6176595.1"
                     /translation="MRFLSRTPVRTFLIYPLVVLLWELLTQAGRIEVNLWGLPLMIWG
                     YLQYRLCGKYRGTRGGGGPGPEVPAERLVSTGLYAYTRNPMYLGHLIFLTGLTLTLKS
                     WFAAVITLAVAIWFHFRVLADEANLARQLGAPYVAYTQRVKRWIPGLF"
     gene            802..1230
                     /locus_tag="VF089_21520"
     CDS             802..1230
                     /locus_tag="VF089_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6176596.1"
                     /translation="MKTVRDILKIKGNDVWRISLEATVFDALQLMAEKEIGALMVMDG
                     ATFVGIISERDYARKVILLGRSSPSTLVKEIMTSHVVYTHLDQSIEECMALVTEKRVR
                     HLPVIDQEKLVGVVSIGDLVKSIIADQKFTIEQLVRYISG"
     gene            1680..1967
                     /locus_tag="VF089_21525"
     CDS             1680..1967
                     /locus_tag="VF089_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX6176597.1"
                     /translation="MDTAALFEKKKEILKIAAKHGARNIRIFGSVARGETRSDSDVDF
                     LVDMEPGRTLFDMGGLLMDLRDLLGLQVDVVTEQGLKPRIRERVLKEAELL"
     gene            1964..2215
                     /locus_tag="VF089_21530"
     CDS             1964..2215
                     /locus_tag="VF089_21530"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX6176598.1"
                     /translation="MRDPKERLHDILEAIANIERYAARGRQAFERDELIQNWFVRHLQ
                     IIGEAAYAVPKELRDQRLDIPWTEIIGMRHILVHDYFVS"
     gene            complement(2328..>2565)
                     /locus_tag="VF089_21535"
     CDS             complement(2328..>2565)
                     /locus_tag="VF089_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176599.1"
                     /translation="KYLKTNDRELLEGSYEIYRESFISVPYPITQGLQPTYEYVAQQR
                     PDVWNHKPEAFMDASFIAELEKTGFIKNLSVGGR"
BASE COUNT          594 a          625 c          713 g          633 t
ORIGIN      
        1 taaatgcccg acgtgcgact cgcggaaagg ttatccggag gttgggccga aatcggaggt
       61 gcgaaaatgg aagagggaat gaccattgcc aaggtgccgg attcgatgac gctcaagccc
      121 gagatgatgc ggcatctgat ccgggagcac gagaaacttg ggcgcacctt gaccgaagaa
      181 gagacagtca cgttgttacg cagcgtggga tttgaaatca ttgagggtaa gtgatgacga
      241 actgaaagct gattgctatt accatgcgct ttctaagccg tactccggtc aggacctttc
      301 tcatctatcc tctggtggtt ctcctttggg aattgctcac ccaagcgggg cgtattgaag
      361 taaacctctg ggggttgccg ctgatgatct ggggctactt gcagtatcgc ttatgcggta
      421 aatatcgcgg gacgcgcggc ggcgggggtc ctggcccgga agttccggcg gagagattgg
      481 tttctaccgg cctgtatgct tatacccgca accccatgta tctcggtcat ctcatttttc
      541 taaccgggct gactctaacc ctcaaatcct ggtttgccgc cgtgatcacg ctggcggttg
      601 cgatctggtt ccatttccgt gttcttgccg atgaagcgaa tttagcgcgc cagctgggcg
      661 cgccctacgt cgcctatacc caacgtgtta agcgttggat cccggggctg ttttaagccc
      721 ggattttcgc tggcggtttc actaccagtg gtttggttgt ataactcgtt tgcggcgact
      781 tattctgaaa ggaagctctc gatgaagaca gttcgagaca tcctaaagat caaagggaat
      841 gacgtttggc gcatctcttt ggaggccacg gtattcgacg ccttgcaact gatggcggaa
      901 aaagaaatcg gcgcgctgat ggtcatggat ggcgctacgt tcgttgggat catctccgag
      961 cgagattacg ccaggaaggt aattctcctg ggccgctcct ctccctcgac gttggtgaag
     1021 gaaataatga ccagccacgt agtttacact cacctcgatc aatcgatcga ggaatgcatg
     1081 gccttggtaa ccgaaaagcg tgttcgccat ctaccggtca tcgaccaaga aaaattggta
     1141 ggtgttgtat ccattggtga tctcgtgaaa tcgatcatcg ccgaccagaa gtttacgatc
     1201 gaacagctcg tgcgttacat cagcggataa aggattgcaa ttccctaagc gttataaaga
     1261 aagaaaagcc gtggcttcgg cgagctgctc tcgtctcgaa gcgacgcaaa attggttgac
     1321 cggttctccg cacttatctc aagcctcatc ctgaagcgag ctccatttct tggttcgtcc
     1381 agcgctccac ctgcctccaa attcttcaag tcgtatcggt caatgggcag ccccggtgtt
     1441 tttcacagga atcaagagat actttagttt gcggtccagt tgccataact gcagatggcg
     1501 ctcacacccc ctggcgatga ctctccaagt tatcgaacac tcgtcgtcag gttctttatt
     1561 gaagccggtt ttttccagct cggcgatgaa tcaagatgtc gctgcgccga actcttatgc
     1621 tggcaaatca gggctccgag gagatacaat taggacgagc cctagcaaaa ggtgaaaaaa
     1681 tggatacggc tgcacttttc gaaaaaaaga aagagatcct taaaatcgcc gcaaagcacg
     1741 gagctcgcaa cattcggatt tttggctcgg tggctcgagg cgaaactaga tcggacagcg
     1801 atgtggactt cctcgtcgat atggaaccgg gtcgcacatt attcgatatg ggcggcttgt
     1861 tgatggatct gcgagatttg ctcggcctgc aagtggatgt tgtgaccgag caggggctta
     1921 agccgcggat ccgggagcgc gtcctaaaag aggctgagct gttgtgaggg accccaagga
     1981 acgcctacac gatatcttag aggccatagc gaatatcgaa cgttatgccg cgcgtggcag
     2041 acaggcgttt gagagggatg agcttataca aaattggttc gttcgccatc ttcagattat
     2101 tggtgaggca gcgtacgccg ttcccaaaga actacgtgat cagcggctgg acatcccgtg
     2161 gacagagatc ataggtatgc ggcatatcct tgttcacgac tattttgtaa gttgacatcg
     2221 acatcgtctg ggatgcggtt gaacgagacc tgccgccgtt aagggataag atcgaaggaa
     2281 tgctgcgcga gctcggaaca aatttctgat ccagccttct agtccagcta acggccaccc
     2341 acgctcaggt tctttatgaa gccggttttt tccagctcgg cgatgaagct tgcatccata
     2401 aacgcttcgg gcttgtggtt ccaaacatcc gggcgttgtt gcgcgacgta ttcgtacgtc
     2461 ggctgcaagc cttgagtgat cgggtaggga accgaaataa aagactcgcg gtagatttcg
     2521 tacgagcctt cgagtaattc gcgatcgttg gttttcaggt attta
//
LOCUS       DASUTG010000927         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_293828, whole genome shotgun sequence.
ACCESSION   DASUTG010000927 DASUTG010000000
VERSION     DASUTG010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293828"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF089_21540"
     CDS             complement(<1..369)
                     /locus_tag="VF089_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEX6176600.1"
                     /translation="MGSDHGGVELKECLAGVLRSKNIDVQDLGTHGSDSVDYPDFGQL
                     VSQQVSQGDADRGILICTTGIGMSIVANKYPNVRAALVQDRETARTSREHNDANVLVL
                     AGATTEPALAREILEIWINTP"
     gene            complement(523..759)
                     /gene="acpP"
                     /locus_tag="VF089_21545"
     CDS             complement(523..759)
                     /gene="acpP"
                     /locus_tag="VF089_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6176601.1"
                     /translation="MASSVQARVKEIVCEQLGVSEDEVVPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDVISYIDNHR"
     gene            complement(792..1535)
                     /gene="fabG"
                     /locus_tag="VF089_21550"
     CDS             complement(792..1535)
                     /gene="fabG"
                     /locus_tag="VF089_21550"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX6176602.1"
                     /translation="MKANGQVALVTGGSRGIGRAIAVALGEAGAYVVINYRGNQEAAE
                     ATLEELHGRGGQGELCRFDVAVETEVDAAVKKIVDGQGKVDILVNNAGVTSDTLLIRT
                     KAQDWDQVIGTNLKGTVLCTKAVCRAMIRQRYGRIVNLSSVVGQTGNAGQSIYAATKA
                     GILGFTKSVARELAARGITVNAVAPGFIETEMTAHLSPQLQEEFLRSIPLGRFGTSEE
                     VAQLVVFLTGPGAAYITGQVMNVNGGLYM"
     gene            complement(1532..2476)
                     /gene="fabD"
                     /locus_tag="VF089_21555"
     CDS             complement(1532..2476)
                     /gene="fabD"
                     /locus_tag="VF089_21555"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6176603.1"
                     /translation="MAITDSVAFLFPGQGSQYVGMGKELCEHYSVAAQAFEEAEQTLG
                     FSLRQLCFYGSEADLQLTENTQPAILALSVAALRVLQSESGVKPAYVAGHSLGEYSAL
                     VCVGALSFSDALRVVRERGRLMQAAVPSGKGAMAVIIGLDMAAVSSICQKAEEGEVVA
                     PANYNGGGQIVIAGSKAAVARAMVLAKSDGAKRVLELPVSAPFHCPLMKPAAEGLKRV
                     LDNVTIHPFSVGVISNVDAEVNLDPARVKPTLAEQAMRPVRWEESVHKLESLGVSKML
                     EVGPGRVLKGLVKRISPKIEVDTFEAPQDLARLRNYIT"
     gene            complement(2521..3558)
                     /gene="plsX"
                     /locus_tag="VF089_21560"
     CDS             complement(2521..3558)
                     /gene="plsX"
                     /locus_tag="VF089_21560"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX6176604.1"
                     /translation="MPKIAVDAMGGDHAPAVVVEGALLAAQDVDAELILVGDKPAVEQ
                     ECLRIRGKLPPFPIVNASQIVPMHESPSVAVRKRDSSMKVAFELIKHGEVQAVVSAGN
                     SGAMMAIGMLVIGTLPQVSRPAILAIVPGPSKGTIIIDAGANVDCKPRQLLQFGVLGS
                     VYAECGLGIVNPRVGVLSNGEEDSKGNELTRAASELLDGAPLNYIGYVEGRDFFNGKV
                     DVIVCDGFTGNVALKTMEGVASFAGEILRSGFEKNWLSRLGYLMTRQSLRHAYRKLDY
                     AEYGGARLLGLNGVAIVAHGGSTALAIKNAIRVASESVSQNVNGHINAALESMVQPVN
                     ESKILNTTIRP"
     gene            complement(3573..3755)
                     /gene="rpmF"
                     /locus_tag="VF089_21565"
     CDS             complement(3573..3755)
                     /gene="rpmF"
                     /locus_tag="VF089_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX6176605.1"
                     /translation="MPVPKRRTSKRVKNQRRSHDGLTQPQWSTCSQCGETVLPHRACR
                     ACGFYRGRAVLKVDES"
     gene            complement(3815..>4172)
                     /locus_tag="VF089_21570"
     CDS             complement(3815..>4172)
                     /locus_tag="VF089_21570"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX6176606.1"
                     /translation="DGRFEGCCSRCLADYSFDVQKSFDIVLVPDPARPERKVEELRRE
                     ELGLSYYSSDMIELTPLIEEQILLALPTRPLCREDCRGLCGGCGVNLNVEECACDPAA
                     PSNSHFAIFRTLKISR"
BASE COUNT          928 a         1263 c          999 g          982 t
ORIGIN      
        1 aggcgtgtta atccagatct ccaaaatctc ccgggcgagg gcaggttcgg tggtggctcc
       61 ggctagaacc aaaacatttg cgtcattgtg ctcgcgactg gtgcgcgctg tttcgcgatc
      121 ctgcaccaag gcggcccgaa cattgggata cttgttggct acgatggaca tcccaattcc
      181 cgtggtgcag attagtatcc cccgatcggc atctccttgt gagacttgct gcgacaccag
      241 ctggccgaag tcgggatagt cgaccgagtc actgccgtgg gtaccgaggt cctgcacatc
      301 gatgtttttt gagcgaagca cgcccgctaa acactctttg agttctactc cgccgtggtc
      361 actacccata gcaatcatga cagttatcga gacagcttct tcttcccgtt aaagcgagac
      421 tgcagaacgc tttgtacatt cctgccagcc caagtcctaa atggcgaaag ctccagcggc
      481 gagacagtat ctcctcgccg aaagctctaa tcgaaacagc agctaacgat gattatcgat
      541 gtatgagata acgtcctgga cagtcttgat tttctcggcg tcctcgtccg aaatctccat
      601 ctcgtactct tcttccaaag ccatcaccag ttcgacaata tctaacgaat ccgcgcctag
      661 atcctcaatg aaagatgctt cagggaccac ctcatcctca cttaccccca attgttcaca
      721 cacaatttct tttaccctag cttgcaccga tgacgccatt ttgtttcctc ctgctttatt
      781 cgccattctt gctacatgta aagtccgcca ttaacgttca tcacttgccc ggtgatatag
      841 gccgctcctg gtcccgtgag aaagaccacc agttgcgcca cttcctcgct tgtgccgaat
      901 ctacccaggg gaatcgatcg aagaaactcc tcctgaagtt gcggcgatag atgagccgtc
      961 atctcggttt cgataaagcc cggagccacc gcattcaccg tgatcccgcg cgctgcgagt
     1021 tcccgtgcaa ctgatttggt gaacccgaga atccctgctt tcgtggccgc gtagattgat
     1081 tgtccggcgt tgccggtctg tcctactacc gaagaaagat taacgatcct gccatagcgc
     1141 tggcggatca tggcacggca aaccgccttg gtacagagaa ccgtaccttt aagattcgtc
     1201 cctatgacct gatcccaatc ttgggccttt gtgcgaatca gaagagtgtc agaggtcacc
     1261 ccggcattat taaccagaat atcaactttc ccttgtccgt caacgatttt cttgacagct
     1321 gcatcgacct cggtttcgac tgcgacatcg aaccggcaaa gttctccctg cccacccctg
     1381 ccgtgaagct cttccaacgt tgcttcagcc gcctcttgat tgccgcggta attgatcact
     1441 acataggctc ccgcttcacc gagagccacg gcgatcgccc ggccaatgcc gcgacttcca
     1501 cctgtcacca aagctacttg cccgtttgct ttcatgtgat gtagttcctc aagcgggcaa
     1561 gatcttgcgg cgcttcgaac gtatcaacct ctattttcgg actgattcgc ttcaccaatc
     1621 cttttagaac tcttccagga ccgacttcaa gcatttttga aacaccgaga ctctccaact
     1681 tatgaacgga ctcttcccaa cgtaccggtc tcatcgcctg ctctgcgagc gttggcttga
     1741 cccgagcggg atcgagattc acttcggcgt caacgttgct gatgaccccg accgagaatg
     1801 gatgaatcgt cacattatcc agaacacgct tcagtccttc tgcggccggc ttcatcagtg
     1861 gacagtgaaa tggcgcgctg accggcagtt cgagcacccg ctttgcgccg tcggacttgg
     1921 ctagaaccat tgcgcgggcg acggcagcct tggaaccggc gatcacgatt tgaccgccgc
     1981 cgttataatt ggcaggtgcg accacttctc cctcctctgc cttctgacat atagagctga
     2041 ccgccgccat gtcgagacca atgatcacgg ccatggcgcc ctttccggat ggcacagccg
     2101 cttgcatcag acgaccgcgc tcgcgcacga ctcttagggc atcgctgaac gagagagcgc
     2161 caacgcacac cagcgcgctg tattcaccga ggctatggcc agcgacatac gccggtttga
     2221 ccccggattc cgattgcaaa acgcggagcg cggctacgga gagagctaaa atggccggct
     2281 gtgtattctc agtgagttga agatcggctt cggacccgta aaaacatagc tgccgaagtg
     2341 aaaatccgag ggtctgttcg gcttcttcaa aggcttgcgc ggcaactgaa tagtgctcgc
     2401 acaattcttt gcccataccg acatactgcg agccctggcc gggaaaaaga aacgccacac
     2461 tgtccgtaat cgccaattca gtttccttcg ctctccagtt ggcccgatcg catcactcga
     2521 tcatggccgg atcgtagtgt tcaaaatttt gctttcattt acgggctgaa ccatggattc
     2581 caaagccgcg ttgatatggc cgtttacatt ctgtgaaact gattcactgg caacgcggat
     2641 ggcgtttttt attgccaatg cagttgaccc tccatgagcc acaatggcca ccccattcaa
     2701 tcccagtaac cgtgctccgc cgtattcggc gtaatcgagc tttcgataag catgccgcag
     2761 cgactgtcgg gtcattagat agcccaagcg gctcaaccag tttttctcaa agccacttct
     2821 tagaatttcc ccggcaaagc tcgccacgcc ttccatggtc ttcaacgcca cgtttccagt
     2881 gaagccatcg catacgatca cgtccacctt accattaaag aagtcccggc cttcgacgta
     2941 cccgatataa tttaacggag cgccgtcgag gagctcgctg gccgctcggg tcaactcatt
     3001 ccctttgctg tcttcttcgc cgttgcttag cacaccgacc cgggggttta cgatacctaa
     3061 accgcactct gcgtacaccg agccgaggac cccaaactgt agtaactggc gtggcttgca
     3121 gtccacattg gctccagcat caatgatgat cgttcccttg gatggtcccg gaacaatggc
     3181 cagaatcgcg ggccggctga cttgaggtaa ggtaccgatc accagcattc caatggccat
     3241 catggcgcct gagtttccgg cgctgaccac tgcttggacc tcgccatgct tgatcaactc
     3301 gaatgcaacc ttcatggacg aatctctctt tctcacggcg acgctcgggg attcgtgcat
     3361 gggtactatt tgggaggcgt taacgatcgg aaaaggagga agcttgcccc taatgcgcaa
     3421 gcattcttgt tcgacggccg gtttgtcccc gacgagaatc aattccgcat cgacgtcttg
     3481 cgccgcaagc agcgcgcctt caacaacaac cgccggggca tgatctcccc ccatcgcgtc
     3541 gactgcgatt ttcggcatag aggaacgaaa tactaactct cgtcaacctt aagcacggcg
     3601 cgaccccgat agaagccgca ggcgcggcaa gcgcgatgag gcaggaccgt ttcaccgcac
     3661 tgcgaacacg tgctccattg cggctgtgtc aggccatcat gcgaccgccg ctggtttttt
     3721 acccgcttcg aagttcgtct ttttggtaca ggcattttga tcctattctt cctgccggca
     3781 aacggcagaa ccaatattca agattggcag taaactagcg actgattttg agcgtccgaa
     3841 aaatcgcaaa atgcgagttg gacggcgcag cgggatcaca ggcgcactct tcaacattga
     3901 gattcactcc acaaccacca cacaatcctc ggcagtcttc acgacacagc gggcgggtcg
     3961 gcaaggccaa tagaatctgc tcttcgatta agggcgttag ctcgatcatg tcgcttgagt
     4021 agtaactcaa acctagctcc tctcgccgca actcctcaac tttgcgctcc ggacgcgccg
     4081 gatccggaac taacactatg tcaaacgatt tttggacgtc aaacgaataa tcagctaaac
     4141 accggctgca gcatccttcg aatctgccgt cg
//
LOCUS       DASUTG010000928         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1479137, whole genome shotgun sequence.
ACCESSION   DASUTG010000928 DASUTG010000000
VERSION     DASUTG010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479137"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VF089_21575"
     CDS             <1..627
                     /locus_tag="VF089_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX6176607.1"
                     /translation="GSIIKLMEAVDSFIKEPTRDVDKPFIMPVEDVFTISGRGTVVTG
                     RVERGVIKVGEEIEIVGFKPTQKTVATGIEMFRKLLDEGRAGDNIGVLLRGTKREEVE
                     RGQVLAKPASITPHTKFNAEAYVLTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVKLPE
                     GTEMVMPGDNINVEIELITPVAMDDGLKFAIREGGRTVGAGVVTKIIQ"
     gene            651..803
                     /gene="rpmG"
                     /locus_tag="VF089_21580"
     CDS             651..803
                     /gene="rpmG"
                     /locus_tag="VF089_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6176608.1"
                     /translation="MRDIVGFACDQCKRKNYTSTKNKKKTTDKLSLKKFCAACRSHTI
                     HREIKV"
     gene            816..893
                     /locus_tag="VF089_21585"
     tRNA            816..893
                     /locus_tag="VF089_21585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:851..853,aa:Trp,seq:cca)
     gene            906..1121
                     /gene="secE"
                     /locus_tag="VF089_21590"
     CDS             906..1121
                     /gene="secE"
                     /locus_tag="VF089_21590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX6176609.1"
                     /translation="MADWIQKVQDTLRHTIEFVKESWQELQKVHWPSRKETYAATGLV
                     IIVVVLVSIYLAIIDLGLTTLIQVIID"
     gene            1138..1668
                     /gene="nusG"
                     /locus_tag="VF089_21595"
     CDS             1138..1668
                     /gene="nusG"
                     /locus_tag="VF089_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX6176610.1"
                     /translation="MAKQWYIVHTFSGFEQKAKAALEERVRTLGKQEYFGEILVPVEK
                     VVELVKGKKKTSSRKFFPGYILVQMELNDDTWHVVKETPKITGFVGGSTNPPSISEEE
                     VRAITLQMEEGAIKPKPRVLFSVGENVKVVDGPFADFNGVVEEVRPDKGKLRVLISIF
                     GRATPVELEFVQVERN"
     gene            1686..2111
                     /gene="rplK"
                     /locus_tag="VF089_21600"
     CDS             1686..2111
                     /gene="rplK"
                     /locus_tag="VF089_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6176611.1"
                     /translation="MAKKVIAEIKLQIPAGAANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQSQQGMITPVIITVYADRSFTFVTKTPPASVLLKKAAGVEKGSKEPGKTKVGKVTR
                     AQVRQIAELKLKDLTANDLAAAEKSVEGTARSLGLDVEG"
     gene            2171..2875
                     /gene="rplA"
                     /locus_tag="VF089_21605"
     CDS             2171..2875
                     /gene="rplA"
                     /locus_tag="VF089_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6176612.1"
                     /translation="MSGFGKSYRAALAKVDRSQRYQLDEGLKLVRETARAKFDETVEL
                     AIRLGVDPRQADQNVRGTVTLPHGMGKAVRVLAFAKGEKEKEAQEAGADFVGSDELIK
                     KISEGWFDFDKAVATPDMMAAVGRIGKVLGPRGLMPNPKTGTVSADIARAVKEIKAGK
                     LEFRVDKAGIVHVPVGKASFEPEKLIDNARAVLSAILRAKPASAKGNYIRGVTLSTTM
                     GPGVKIDLSQVRAMAA"
     gene            2925..3467
                     /gene="rplJ"
                     /locus_tag="VF089_21610"
     CDS             2925..3467
                     /gene="rplJ"
                     /locus_tag="VF089_21610"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6176613.1"
                     /translation="MAELEAARASHGEKAETVAAVHEKFRRAKMAIATEYRGLSVSKM
                     TQLRREIRDVAGEYQVIKNTLVRRALKDTAYGELERMLEGPNGWVFAYDDPVLLSKTL
                     VKFVDANEKMTIKAAMFEGELMDPARVKVLSQMPSKPELQATLLALLQAPATHLLRLM
                     QEPGSRVVRLLDAAAKGKAE"
     gene            3510..3887
                     /gene="rplL"
                     /locus_tag="VF089_21615"
     CDS             3510..3887
                     /gene="rplL"
                     /locus_tag="VF089_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6176614.1"
                     /translation="MAEVSREQVKDYIKNMTLLDAATLVKELEEELGVSAAAPVAVAA
                     AAAGPAAAAEEKTEFDVVLAEAGEKKIQVIKVVRELTGLGLKEAKDLVDGAPKNVKEG
                     VAKAEAETIKKKLEEQGAKVDIK"
BASE COUNT         1039 a          884 c         1171 g          896 t
ORIGIN      
        1 gggtctatca taaagctcat ggaagctgtt gattccttca ttaaagagcc taccagggac
       61 gtagacaagc catttatcat gccggtcgag gacgttttca cgatcagcgg tcgaggcaca
      121 gttgtaaccg gccgggtcga gcgcggcgtc atcaaggtcg gggaggaaat tgaaattgtc
      181 ggctttaagc cgacgcaaaa gacagtagct accgggatcg agatgttccg caagcttttg
      241 gacgagggtc gggccggcga taatatcgga gtgcttctac ggggaaccaa gcgtgaagag
      301 gtcgagcgag gtcaggtctt ggcgaagccc gcgagcataa cccctcatac taaattcaat
      361 gctgaggctt atgttctaac gaaagaagaa ggtggacgac ataccccgtt tttcaacgga
      421 tatcgaccgc aattctactt ccgcacgacc gacgttaccg gtgtcgtgaa actccccgaa
      481 ggaaccgaga tggttatgcc cggggacaac atcaatgtcg agattgagct aattactcct
      541 gtcgctatgg acgacgggct taagtttgca attcgagagg gcggtcggac cgtgggcgct
      601 ggcgttgtca caaagattat ccagtaactt agctcaaggg gggcgcactc atgcgggata
      661 tcgtagggtt tgcttgcgac caatgtaagc gaaagaatta cacatcgacg aagaacaaga
      721 aaaaaaccac cgataagctt tctttaaaga agttttgcgc tgcgtgtcga tctcacacga
      781 tacatcgaga gatcaaggtt taacgattca ccggggggtc agtagctcca actggctaga
      841 gctccggact ccaaatccgg ctgttgcagg ttcgaatcct gcctgacccg ccataatgta
      901 gcctcatggc agattggatt caaaaagttc aggacactct tcgccatacg atcgagttcg
      961 tcaaggagtc ctggcaggag ctgcaaaagg tccattggcc gtcgcggaag gagacttacg
     1021 ccgcgaccgg actcgttatc atagtggtcg tcctggtgtc gatttactta gcgatcatag
     1081 acttgggtct gaccacattg attcaggtta tcatcgacta actctccgag ctctcctatg
     1141 gcaaagcaat ggtacatcgt tcacaccttt tcaggttttg aacaaaaggc gaaagcagct
     1201 ctcgaggagc gggtaaggac tctcggtaaa caggagtatt tcggtgaaat tcttgtgccg
     1261 gttgaaaagg tcgttgagct cgtcaaggga aagaagaaaa cttcctcaag aaaatttttt
     1321 cccggttata ttctcgttca aatggagctc aatgacgata cctggcatgt ggtgaaggag
     1381 acgccgaaga ttactgggtt tgttgggggc agcacgaatc cgccatcaat ttcggaggag
     1441 gaggttagag ccatcactct tcagatggaa gagggcgcaa tcaagcctaa gccgagggtg
     1501 ctattttcgg tcggtgagaa cgtgaaagtt gtcgatggtc cgtttgcaga tttcaacggg
     1561 gttgttgaag aagttaggcc cgataaaggc aagcttcgag tgctcataag tatttttgga
     1621 cgagccactc ctgtcgagtt ggaatttgtt caggttgaac ggaattagtt taacaaggac
     1681 accaaatggc taagaaagtc atcgcggaaa tcaagttgca gatccctgct ggggcagcca
     1741 acccaagccc tcccgtcggc cccgcattgg ggcagcgtgg cgttaatatt atggaattct
     1801 gcaaggcctt taatgcggca actcaatctc agcaaggtat gatcactccg gtgattatca
     1861 cagtctatgc ggaccggtct ttcacgtttg tgacgaagac cccgcccgct tctgtactcc
     1921 taaaaaaagc tgcaggcgtg gaaaaaggtt ctaaggaacc aggcaagact aaagttggga
     1981 aagtgactcg ggcgcaagtt cggcagattg cggagttgaa gctcaaggat ctgaccgcta
     2041 atgatttggc cgccgccgag aagtcagttg aagggactgc tcgcagcctt ggtctcgatg
     2101 tcgagggata attttagagc gcagtcaatg gtttgatcca tctgcttcga agcgattttg
     2161 agggggacac atgagcggtt ttggaaaatc gtatcgcgct gcgttggcaa aagtagaccg
     2221 tagtcaacgc tatcaattgg acgagggtct taaacttgta cgtgagaccg cccgggcaaa
     2281 gtttgatgag accgttgagt tagccatccg cttgggggtt gatccgcggc aagctgatca
     2341 aaacgtccgg ggaacggtga cattgccgca cggcatgggc aaggccgtta gggtgctcgc
     2401 ttttgccaag ggagaaaaag agaaagaagc gcaggaagcc ggagcggatt tcgtcggaag
     2461 cgatgagctc attaagaaga taagcgaggg gtggttcgat tttgataaag ctgtagcgac
     2521 tccggatatg atggccgcgg tcggtcgaat cggcaaggta ttgggccctc gcggccttat
     2581 gcctaatcct aagacaggta ccgtctcggc cgatattgcg cgtgcggtta aagagatcaa
     2641 ggccggcaaa ttggagtttc gagtggataa agcaggaata gttcatgttc ctgtgggcaa
     2701 agcttctttc gagcccgaga agctcataga caatgctaga gccgtgctct ccgcaattct
     2761 ccgagccaaa cctgcctccg ctaaaggcaa ctatattcgc ggtgtgacgc tatctacaac
     2821 gatggggcca ggagtcaaaa tcgacttatc gcaggttcgg gctatggctg cctagatctg
     2881 ttctttagct aattttcaaa gccaaggaat tcgggagtga aagcatggcg gagttagaag
     2941 ctgcacgcgc cagtcacggc gaaaaagccg agaccgtagc ggctgtgcat gagaaattta
     3001 ggagagcaaa aatggcgatc gcgacggagt atcgcggact ctccgtaagt aagatgacac
     3061 agttgcggcg cgagattcgt gacgtagctg gggaatatca ggttatcaaa aatactctcg
     3121 ttcgccgggc gcttaaggat acggcctacg gtgagttaga acggatgctt gagggtccca
     3181 atggatgggt gttcgcgtat gatgatccgg tcctgctttc taagacgttg gttaagttcg
     3241 ttgacgctaa tgaaaagatg acgatcaagg cggcgatgtt tgagggcgag ctaatggacc
     3301 cggctagggt caaggtcctc tcacaaatgc caagcaaacc tgagctgcag gcgaccttgc
     3361 tcgcgctgtt gcaggcgccg gcgactcact tgcttcgcct gatgcaagaa cccggatctc
     3421 gagtggtcag attgctagat gcggccgcta agggaaaggc cgagtaacaa acgatagttg
     3481 tttggaatca atcaaaaggg ggacggttta tggcagaggt atccagggaa caggttaaag
     3541 actacatcaa aaatatgacg ctgctggacg ctgcaactct agttaaggag ctcgaggagg
     3601 aactcggggt cagtgctgcg gcgccggttg cggtagctgc ggcagctgct ggtccggcgg
     3661 cagcagcgga ggagaaaacc gagtttgatg ttgttcttgc agaagcgggc gaaaagaaaa
     3721 tacaggttat caaggttgtt cgcgaactta ccggtcttgg tttgaaagag gcgaaggatc
     3781 tcgttgatgg cgcgccgaag aatgtcaaag aaggggtagc caaagccgag gctgaaacga
     3841 tcaagaaaaa gcttgaagaa cagggagcta aggtcgacat caaatagcgc accagcacca
     3901 cccgggactt tgttcgtgac acggtactac tgtttagatc gctaaaggag agacaatgcc
     3961 atttcaagtg gccaataatt tacggtttcg
//
LOCUS       DASUTG010000929        11822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1775733, whole genome shotgun sequence.
ACCESSION   DASUTG010000929 DASUTG010000000
VERSION     DASUTG010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775733"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF089_21620"
     CDS             complement(<1..272)
                     /locus_tag="VF089_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX6176615.1"
                     /translation="MALSFWFDFASTYSYPAAMRIEQLAQNCGVPLSWNAFLLSRIFS
                     AQGWNDSPFNLYPGKGRYMWRDLERICEAQHVPFRHPSQFPRNGLLG"
     gene            complement(339..833)
                     /locus_tag="VF089_21625"
     CDS             complement(339..833)
                     /locus_tag="VF089_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX6176616.1"
                     /translation="MDYQSMTRSLKSRSVSPGIVLGLGLGGFIDGIALHQIAQWHNMG
                     SAKLPPVTMEAMQQNMAWDGWFHVATLVLTIIGAYLLLSHARAGRLLPDTRSFTGRLL
                     LGWGAFNLVEGIIDHHILEIHHVRDLPMHVPIYDWLFLGIGGVGLLLIGWWLARRNAD
                     VHGA"
     gene            1230..2309
                     /locus_tag="VF089_21630"
     CDS             1230..2309
                     /locus_tag="VF089_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176617.1"
                     /translation="MKPKKTGTLVLCAAIISAVWVNVTFSAETPFYHGKTLTVLINFA
                     AGGPTDIEGRLVARFLGKHVPGQPTIVAQNMPGAGGVTGTNYLGEIAKPDGLILGYFT
                     SQFFNILTADPNLRVDLAKFAYIASIEGVAVAYVRKDVPPGIQEAKDILKAKGLRAGG
                     LSLNSAKDVRFRLQLDILGVPHQYVTGYNSNSDARLAVERNEIQFFTEGTPGYRSQVQ
                     PRMVKDGVVMPLYADELVTVDGEFRPSAEVPDIPSFSQLHQKIFGKLGSGPLWETLKT
                     INLTHAMQRIAKVAPNTPPEAVAALRQGFSQMLKDQEFIQEFKKVTRSDPNFQVGGEA
                     DKLINKLVQAPPEVKKTIRKYTESK"
     gene            complement(2535..3005)
                     /locus_tag="VF089_21635"
     CDS             complement(2535..3005)
                     /locus_tag="VF089_21635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6176618.1"
                     /translation="MDNVEIAYLADYAAVVVELAAALHAQDREYYGARTPADIAADLV
                     LSLQREVLPLALIALRDGVLLGAAILRQDSITTHPHLGPWLTAFYVRPELRGRSIGGK
                     LVAAIEAAAQRLGVAQLYAGSGRAASLFERAGWQVMERVSYHDEEIAILRRDLR"
     gene            complement(3075..3386)
                     /locus_tag="VF089_21640"
     CDS             complement(3075..3386)
                     /locus_tag="VF089_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX6176619.1"
                     /translation="MRFLVKVNIPVEAGNQAARAGKLGATIQSILADLKPEAVYFTDD
                     SGQRTAFIFVEMNDASQIPAIAEPWFLALNASIEIHPVMIPDDLARASAAIEAAAKKY
                     S"
     gene            complement(3427..5559)
                     /locus_tag="VF089_21645"
     CDS             complement(3427..5559)
                     /locus_tag="VF089_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176620.1"
                     /translation="MAVKDWLDTGKCLLGFHQGDWRPESPVRCVLIQTCERCGTVNQR
                     VEHNWSDWNQAGAQSCELSRSCSRCAESEKRIEHNSEAWSYRQDGSCDQSKRCQRCAE
                     WNDENRIEHQWGFWEYSERYRAPLHSCGRCGLSASYFADQKIDQDEVSSTNGDGARKE
                     LDELLSDDRAIEEMLARAEERVGSSEPDVTPIPGPDDDTSDWRQQMGLLRQMYQGFIA
                     SGQIAPERQTFLTSILAELEEILGSPATDLADKQIRATRVQQLLSQISEALLHPSRAQ
                     PLEEPIAGSRRAALIELHANLHRYVMTEASSGLLTGEEGKALMALMGRLRESREALAN
                     LSNSADSVRVETEVLRPLALEIRNFSLRHHLTLAQPVWPSRAVGQHGNALFYSGGSDL
                     GALVVQVCASRELRCLMPQADREPASLRWDQLRESAVAVFDFSAYKRAASLEEASVIA
                     AVAYELGIALALGRAAVVVATDGQDLPFDLDIEPVCLNNDDSDVANLSAAFDRALYGL
                     QRGAAGSSVKSSVRYLRERFAGHSSRHVRMSLETLDDETMADPIKVKHLITSTFGFLG
                     AEALQILLSAWPGQYPDTSSKRCFHVTAFGPTWANSTRRLVGDACGSQSEYIRGDGVL
                     APDILRSIWDEICRATHVVVDLTGLNANVVLELGIAHTLGRNVLLISQDQQVERYFRA
                     IAKHRIHSYTLRESEAPALQATLQRFLT"
     gene            complement(5579..6745)
                     /locus_tag="VF089_21650"
     CDS             complement(5579..6745)
                     /locus_tag="VF089_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX6176621.1"
                     /translation="MFKDLFDKGKCALGFHTGEWRYHQDRSCQQTRICSRCKVASQQL
                     VHTWQSWNYEKSDDCRMARRCDRCAEVETKIEHVWGSPIYASEQSCALVRPCSRCSET
                     SPAGNAHVWQSWSYEAADHCSQLVTCSRCGEAGTERRTAHDWGSWHPSQFYEAPVRVC
                     RRCAEMVFESDHGGGEADQVSLQMIDRAVWNVMEATDSAGVRERLSQNKKVLFTPVAE
                     KYFKFVIDQRAPDEGARNTFRQLAGLIERCRREGIETVFAPAASQDSAYDPPSSSTSP
                     GRTPSPAPAGEKNARLFGHWRHTEPRSSGGFSMTIDTHCVLDPGGRFEWWSKSMSSFG
                     PSTSGPENGTWSASGNTLQLKFDDGTSLAREVALKDTTMFWPGDGRYRIWERIS"
     gene            complement(6917..7693)
                     /locus_tag="VF089_21655"
     CDS             complement(6917..7693)
                     /locus_tag="VF089_21655"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX6176622.1"
                     /translation="MPGKKTIFVLILIVGAAALYFWRGGEKPKPQVVAPLDAPAEPLI
                     RYPIDEASLADEPLEAAAKPLPSLDESDSLMQDILAQLFGGKREKLFNFQNIIRRAVA
                     TLDNLPRENLSMQLMPVKPVTGRVITAGQGSSLTLAAANDARYDAYVWLAEAVPTGAL
                     VAAYVQVYPLLQQDYEQLGYPGRYFNDRVVQVIDHLLNTPEVQAPVLLIQPKVLYEFA
                     DPKLEELSAGQKILVRMGHENAVKVKAKLREIRSALVPKG"
     gene            complement(8184..9416)
                     /locus_tag="VF089_21660"
     CDS             complement(8184..9416)
                     /locus_tag="VF089_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX6176623.1"
                     /translation="MAKKSCLLVLTAALLCSIVPAHSQTQPNLPDEPGKEHVVAYCSG
                     CHGLGRVANSSYSQAYWHTAVRMMLNFGVLIPPEQITLVTDYLAKSFPEKPKPVANII
                     PGAARIEIKEWQVPTPGSRPHDPLATRDGAIWYTGQMSNKLGRVDPKTGEVKEYPLKT
                     AQTGPHGLVEDKDGNIWFTGNHQALVGRLDPKTGAVTEYKMPDPKATDPHSLAFDQSG
                     ILWFTLQQSNMIGRLDPSSGQIKLVTAPTPRSRPYGIMVNSKGIPFVVLFGTNKIARI
                     DPKTLEIREYTLPDAKARPRRMAFTSDDIVWYSDYPRGYLGRLDPATGQVTEWQSPGG
                     QRSEPYGIVASKDVIWYSESGTKPNTVVRFDPKTQNFQTWVIPSGGYIVRKMDVTPDG
                     NPVLATSTVNGVALVEIK"
     gene            complement(10315..11382)
                     /locus_tag="VF089_21665"
     CDS             complement(10315..11382)
                     /locus_tag="VF089_21665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6176624.1"
                     /translation="MTSQAIIAPRIKAALDACVAAGSPGAIIAIDAPSLGIAFSSARG
                     LFARGQSRPLRPNDPFRAASVTKAVTAATAVCLAAERRWDLDSPVTPFLPPDLVELLR
                     DLEGLEDLDALTIRRLLSHTSGLPDYFFRAQFQARVHADPNRIWRPAELVEAAIEGNR
                     LAFLPGTNFSYGDTGYVLVGLAIEGLAGCPLADAYRSLIFARLGMDATYLEWHEPSRS
                     SDVSHHYDGQRDLLPMNTSFDWAGGGLVTTAADLIRFLRGLFGGTLFDERWSAELTSW
                     RDGLRWPPNSSARYLRYGLGIGVNLACGEEVIGATGVWGAFAYFWPAGGAGITGTVNL
                     RGADRTTLLDAVVCALRSVRR"
BASE COUNT         2585 a         3430 c         3218 g         2589 t
ORIGIN      
        1 cccagtagcc cattgcgcgg aaactgcgat ggatggcgga atggcacgtg ctgcgcttcg
       61 cagatgcgct ccagatcacg ccacatgtaa cgccccttcc ccgggtacaa gttaaacggc
      121 gagtcgttcc atccctgcgc actgaaaatt cggctgagta agaaggcgtt ccacgacagc
      181 ggcacaccgc agttttgcgc gagttgctcg attcgcatcg cggccgggta tgagtacgtg
      241 ctcgcgaaat cgaaccaaaa ggaaagtgcc atgcctgtac ccgcagctta ttcgtttgcg
      301 gtgttgatat ttgcttcggg agttaactta ctcaagtctc aggccccgtg aacgtcagcg
      361 tttcgacgtg cgagccacca gccgatgagc agcaaaccga cgccgccgat accgaggaac
      421 agccaatcgt agatcggcac atgcatcggc aggtcgcgca cgtggtggat ctcaaggata
      481 tggtgatcga tgatcccttc gaccaggttg aaagcacccc agccgagcaa cagccggccg
      541 gtgaatgatc gagtatccgg cagcaggcgc cccgctcgcg catggctcag tagcaaataa
      601 gctccgatga tcgtgagcac cagcgtggcg acgtgaaacc acccgtccca agccatgttt
      661 tgttgcatcg cctccatcgt caccggcgga agcttggccg accccatgtt atgccattgc
      721 gcgatctggt gtagcgcaat gccgtctatg aaaccgccga ggccaagccc taacacaatt
      781 ccgggactga ccgagcgcga ttttagtgag cgagtcattg attgataatc catcgctcct
      841 ccttgcgaaa gtcgcccaac ccactgaaag ccactgccac gttgtgggct gcgtgctgcc
      901 cgtcttttgc ttgatgtaca gcccacacgt ttacaacaaa cctcttcgta tcaatcgatc
      961 tgaaggaatt cttggatcga acatccgctc tcaatcaact cttgcagttg cacgattctg
     1021 ccgcaagggg aaaatgcgac tccgttcatc cgggcgctgc cgggataggc aaggaacccg
     1081 gcagtaatcg tcttgccggt tccacggaaa aatcatattt tggcccagtc ttgtatgtat
     1141 ctggaaggcg gctcgtgaat ggagactgac acattcaagc acagttcaac aatcaagagt
     1201 ctggcttccc aaccaagaaa gggacaacga tgaaaccgaa gaagacagga acccttgtgc
     1261 tttgcgctgc gatcatcagt gccgtctggg taaacgttac gttttctgcc gagacgcctt
     1321 tctatcacgg caagacgctg acggtcttaa tcaattttgc cgccggcggg cccaccgaca
     1381 tcgaaggaag gctggtggca cgcttcctgg gaaagcacgt cccagggcag ccaacgatcg
     1441 tggctcagaa catgccggga gcgggcggcg tcaccggcac taattacctg ggcgagatcg
     1501 ccaaaccgga cggattgatt ttgggatatt tcacgtctca gttttttaat attttgactg
     1561 ccgatcccaa cctgcgagtc gatctggcaa agtttgccta catcgcaagt atcgaaggtg
     1621 tggcggtcgc gtatgtacgc aaggacgttc cgcccggcat tcaggaagcc aaagacatcc
     1681 tgaaagccaa ggggttgagg gccggcggtt tgagtctcaa tagcgccaag gatgttcgtt
     1741 tccggctgca gctcgatatc ttgggtgttc ctcaccagta cgttaccgga tacaactcga
     1801 actcggatgc gcgcctggca gtggaacgaa acgagattca attctttacc gaagggacac
     1861 ccggctatcg ctcgcaggtt caaccgcgga tggtgaagga tggcgtcgtc atgccgctct
     1921 atgccgacga gcttgtgacc gtcgatggcg aattccgccc gagcgctgag gtgcccgaca
     1981 ttccttcctt ctcacagctt catcagaaga tattcggcaa gcttggttcc ggcccgctgt
     2041 gggaaacctt gaaaacgatc aatcttacgc acgcaatgca gcgcattgcc aaagttgcgc
     2101 caaacacgcc gccggaagcg gttgcagcgc tgcgacaagg gttttcgcag atgctcaagg
     2161 atcaggaatt catccaggag ttcaaaaaag ttacccgctc cgatccgaac ttccaggtcg
     2221 gcggtgaagc cgacaagctg ataaacaagc tggttcaagc gcctcccgag gtgaagaaaa
     2281 ccattcgaaa atacaccgag agcaagtgag ttgagcccgc tctgtatagc tgacgtaatc
     2341 acgcagaagg atttctgtcg atcgattacg ttcgctgatc cagactttca ttaagcgctt
     2401 ggcgagtcgt attttcacat ccacgcaagg catcgcctcg actctctcac tctttcacta
     2461 gcgggcaatc gcatcgcggg ccatttgtga cccgtcctag gttggtaccc gcaattcacg
     2521 atgttagatc tcgatcatcg caagtcccgg cggagaatcg caatctcctc atcatggtat
     2581 gagacacgct ccatgacttg ccaccctgcg cgctcgaaca acgatgctgc gcggccactg
     2641 ccggcgtaga gctgcgcgac gccgagccgt tgtgccgcag cctcaatcgc cgccaccagc
     2701 ttgcccccga tgctgcggcc ccgaagctcc gggcgcacat agaatgcggt gagccaaggt
     2761 cccaagtgcg ggtgagtcgt aatcgaatcc tggcgcagaa ttgcagcgcc gagcaagacg
     2821 ccgtctcgca acgcgatcag cgccagcggc agcacttcgc gctgaagcga cagcacgaga
     2881 tcggccgcga tatcggcggg cgtgcgcgcg ccatagtatt cgcgatcttg cgcgtgcagg
     2941 gcggccgcta gctcaaccac cacagccgca taatctgcta ggtaggcgat ttctacgttg
     3001 tccatggatg ccgctgcgca tgtacctgag cggcggtagg aagacggctc atcccatgta
     3061 gatcgcgctt gcggtcagct gtacttctta gcggcggctt cgattgcagc actggccctg
     3121 gcgagatcgt ccggtatcat cacagggtgg atctcgatgc tcgcatttaa cgctaggaac
     3181 cacggttcgg caattgccgg gatctgtgaa gcgtcgttca tctcaacgaa aataaacgcc
     3241 gtccgttggc cactatcgtc ggtgaaatac acggcctcag gcttcaaatc cgccagtatg
     3301 gattggatgg ttgcaccgag cttgccggct cttgcagcct gattcccagc ctcgaccggg
     3361 atgtttacct taactagaaa tcgcataaat catctcctct aatcaacagt cacaaaccca
     3421 tttttgctag gtaagaaatc tttggagtgt cgcctgcaaa gcgggggcct cggattctct
     3481 gagtgtgtac gagtgtatgc ggtgttttgc aatggcgcga aaatagcgct cgacttgttg
     3541 gtcttgcgag atcagcaaaa cgttgcgtcc aagcgtatgc gctattccga gttcaagcac
     3601 gacgttggca tttagtccgg tgagatcaac cacgacatgg gtggcacggc atatttcatc
     3661 ccaaatcgaa cgcagaatgt ccggagccaa gactccatcg ccgcggatat attcactctg
     3721 cgaaccgcag gcgtctccga ccagacgtct cgtgctgttc gcccatgtcg ggccgaaggc
     3781 ggtcacgtga aagcaacgtt tactcgacgt gtccggatac tgacccggcc aggccgacag
     3841 caggatctgc aaagcttcag cgccgagaaa gccaaatgtg gaggtgatca gatgtttgac
     3901 tttgatcgga tcggccatgg tttcgtcatc cagcgtctca agggacatgc gcacgtgacg
     3961 actggaatgg ccagcaaatc gctcgcgcaa atatcttacc gacgatttta cggaactgcc
     4021 ggctgcgcct ctttgcaagc cgtacagcgc tcgatcaaag gcggcagaca gatttgcaac
     4081 atcgctatca tcgttattca gacaaaccgg ttcgatgtcc aaatcgaacg gcagatcttg
     4141 accatcagtt gcgacaacga ctgcggcgcg accgagcgca agcgcgatgc ccagttcgta
     4201 ggctacggcc gcaatcacgc tcgcttcttc taaagaagcg gcgcgcttgt aggcgctaaa
     4261 atcaaagact gccaccgcag attcacgcaa ctgatcccaa cgcagcgatg ctggttctcg
     4321 atcggcttgc ggcatgagac accgaagctc gcggctggcg caaacctgca cgaccagggc
     4381 gccgaggtcg gagcccccgg aatagaacaa agcattgcca tgttgcccaa ccgctcgcga
     4441 cggccacacg ggttgggcga gggtcagatg atggcgcaag gaaaaattgc gaatttctaa
     4501 cgccaacggc cgcagcactt cggtttctac tcttaccgaa tcggcgctgt tcgatagatt
     4561 tgcgagagcc tcgcgactct ccctgagccg tcccatcaaa gccatcaagg ctttgccttc
     4621 ttcgccggtt aagagccccg acgacgcctc ggtcatgaca tagcgatgaa gatttgcatg
     4681 cagttcaatt aaggccgccc ggcgcgagcc tgctataggt tcttcgagtg gttgtgctct
     4741 gctcggatgg agaagcgctt cggatatctg cgaaagcaac tgctggacac gggtcgctct
     4801 gatctgtttg tcggccagat ccgtcgctgg gctgccgaga atctcttcaa gctctgccaa
     4861 gatcgacgtc aagaaagttt ggcgctccgg agcaatttgg ccgctggcaa tgaacccctg
     4921 gtacatctga cggagaaggc ccatctgctg ccgccagtcg gaggtgtcat cgtccgggcc
     4981 gggtatcgga gtcacgtcag gttcggaact cccgacacgt tcttcggccc tggcgagcat
     5041 ttcttcgatc gctcggtcat cggataagag ttcgtcgagc tccttccgcg cgccatcacc
     5101 attggttgaa gagacttcgt cctgatcaat tttctgatcg gcaaagtaag aggcgctcaa
     5161 tccacagcgg ccgcagctgt gcagcggcgc acgataacgc tcactatact cccagaatcc
     5221 ccattgatgt tcgatgcggt tttcatcatt ccactcagcg caacgctggc aacgtttgct
     5281 ctgatcgcaa gaaccatcct gccgatagct ccaggcctcc gagttatgtt caatacgctt
     5341 ttccgactca gcgcagcggc tgcagctgcg cgacagctcg caagattgtg cgccggcctg
     5401 attccaatcc gaccagttgt gctctactcg ttgattgacg gtaccgcagc gctcgcaggt
     5461 ttgaatcaag acgcagcgca ccggggattc gggccgccaa tctccctgat gaaaaccaag
     5521 caggcacttt ccggtatcga gccaatcctt cactgccata agttttccgc agtcgaattc
     5581 acgagattcg ttcccatatc cgataacgcc catcgcccgg ccagaacatg gtggtatctt
     5641 tgagagcgac ttcgcgcgcg aggctagttc catcgtcgaa cttaagctgc agcgtattgc
     5701 ctgacgccga ccaggtgcca ttttcgggtc cagatgtact cggcccgaaa ctgctcatcg
     5761 atttactcca ccactcgaag cgaccgccgg ggtctaaaac gcagtgggtg tcgatggtca
     5821 tgctgaatcc gcctgatgac cgcggttctg tatgacgcca gtgcccgaag agccgcgcgt
     5881 ttttttcccc ggccggcgct ggcgatggcg tacgcccggg gcttgtgctc gatgatggcg
     5941 gatcataggc ggaatcctgt gatgcggcag gcgcaaaaac cgtttcgatc ccttccctgc
     6001 ggcatcgctc gatcaaaccg gcgagctggc gaaaagtatt cctggcacct tcatccggcg
     6061 cccgttggtc gatgacgaat ttgaaatact tctcggcgac cggagtgaat aagactttct
     6121 tgttctgaga cagacgctca cgcacgccgg cgctgtctgt cgcttccatg acgttccaaa
     6181 ctgcccggtc aatcatctgt aaagagactt gatctgcctc gccgcctcca tgatcagact
     6241 caaagaccat ctccgcgcag cgacggcaca cgcgcaccgg cgcctcatag aactggctcg
     6301 gatgccagga gccccaatca tgggctgtac gtctttcggt tccggcctcg ccgcaacgcg
     6361 agcaggtcac gagttgcgag cagtgatctg ccgcctcgta actccaggat tgccaaacgt
     6421 gagcgttgcc ggcgggtgat gtctcactgc agcgcgagca tggacgaacc agcgcgcaag
     6481 attgctccga tgcatagata ggtgagcccc agacatgctc aatcttcgtt tccacttctg
     6541 cgcaccggtc gcatcgtcga gccatgcggc agtcgtccga tttttcgtaa ttccagcttt
     6601 gccaggtatg aaccagctgc tggcttgcaa ctttgcagcg cgagcagata cgcgtctgct
     6661 ggcatgagcg atcctggtga tagcgccact caccggtatg aaatccgagg gcgcattttc
     6721 ctttatcgaa cagatctttg aacatacggg aaaaatctcg acgggactct ccgcttgttg
     6781 gggcaaaatg tcacaatctc ccagcggctt acatcggtct gcgatctccg gtgctgcagc
     6841 gacccctggg gcaagcatcc tagagggcat ggaaatagta catttctcat gccctcgttc
     6901 gtaacaccgc ctgcggtcat ccctttggca ccaatgcgct gcgaatctct cgcagcttcg
     6961 cctttacttt aacggcattc tcgtggccca tccgcacgag gatcttctgg cccgccgaga
     7021 gttcctctag tttcggatca gcgaactcgt acagcacctt gggttgaatc agcaacaccg
     7081 gcgcttgaac ctccggagtg ttcagtagat ggtcaattac ttgcaccacg cgatcattga
     7141 agtatctgcc tggataaccc agttgctcgt agtcttgttg tagcaacgga taaacttgca
     7201 cgtaggcggc tactagcgca ccggtgggca ccgcctcagc cagccacaca taggcgtcat
     7261 agcgcgcgtc attcgccgca gcgagggtca ggctgctacc ctggcctgcc gtaatgacac
     7321 gcccggtcac cggttttacc ggcattaatt gcatcgacag gttctcgcgc ggcagattat
     7381 ccaaagtggc cacggcgcgg cggatgatgt tctgaaaatt gaaaagcttt tcccgtttac
     7441 cgccgaaaag ctgcgccagg atatcctgca tcaggctgtc gctctcatcc agcgatggca
     7501 gcggtttcgc cgcggcctca agcggctcgt ccgctaaact ggcttcgtcg atcggatagc
     7561 gtatcagcgg ctccgctggc gcatcgagcg gtgcgacaac ctgcggcttg ggcttttccc
     7621 cgcccctcca gaagtacaag gcagccgcgc cgacgatcag aatcaagaca aaaatcgttt
     7681 ttttaccagg cataagtcct cgtgtattct cgcatcgtga ttcaaacctg accgcaccaa
     7741 ttctaggttg cgatagcaaa gcaatacaaa gtttccgtta aaaccgcaga agctgaaacg
     7801 accggggcac gattaattgt cgtgcaatca cctcgagttt gaaggtcgga gttgtttgct
     7861 cggaaaacta tcaacgcaga aagactgttg cagtgtttgc cgatggacgt catctcttct
     7921 ccggtatgcc taaagtccac aatcggtttg gactgtcagc tgagcagggt ttgtggaccg
     7981 ctagtcccga atccagccgt aggctaagac tttagtagag ttgttacaat agcgtacggt
     8041 taaactccgc ttcgacattt gactttattt gactttagcc caccgaagag atttcaaaaa
     8101 gaaactgtaa tcatgtcggc gaaaaatcag cgtaggttcg gcccgattta cctctcgcag
     8161 aactccgccc ttcttttatc gcctcatttg atctcgacga gggcgacgcc gtttaccgta
     8221 cttgttgcca gtaccggatt gccgtcgggc gtgacgtcca tcttgcgcac gatgtagcca
     8281 ccggacggaa tcacccaggt ctgaaagttt tgggtctttg gatcgaagcg cacgaccgtg
     8341 ttcggcttgg tgccggattc gctgtaccag atgacatctt tggaggcgac gatgccgtaa
     8401 ggttcggatc tctgcccgcc gggcgattgc cattcggtca cctgaccggt cgcgggatcg
     8461 agccggccca gataaccgcg agggtaatcc gaataccaga cgatgtcgtc gctggtgaag
     8521 gccatgcgcc tcggccgcgc cttcgcatcg ggcaaggtgt attcgcgaat ctccagcgtc
     8581 ttgggatcga tgcgcgcgat cttgttggta ccgaacagaa caacaaaggg tatgcccttt
     8641 gaattcacca tgatgccgta ggggcgggaa cgcggcgtcg gtgcggtcac aagcttaatt
     8701 tggccgctgc tcggatcaag ccgtccgatc atgttggatt gctgcagcgt gaaccacaaa
     8761 atcccgcttt gatcaaaggc gagcgagtgc ggatccgtcg ccttcgggtc cggcatcttg
     8821 tattccgtca ccgcgcccgt ctttggatcg agtctgccga cgagcgcctg gtgatttccc
     8881 gtgaaccaaa tgttgccatc cttgtcctcg accaaaccgt gagggccggt ctgcgccgtc
     8941 ttgagcggat attccttcac ctcccctgtc ttcggatcga cccgtccgag cttgttgctc
     9001 atttgaccgg tgtaccagat ggcgccgtca cgggtggcaa gcgggtcgtg cggccgcgag
     9061 cctggtgtcg gaacctgcca ctccttgatc tcgatccgcg ccgccccggg gatgatgttg
     9121 gccacgggct tcggtttttc aggaaagctt ttagccaaat aatcggtgac gagagtgatc
     9181 tgttcaggcg gaatcaacac accgaaattg agcatcattc taacagccgt gtgccagtaa
     9241 gcctgcgagt aactagagtt ggcgacacgg ccaagtccgt ggcagccgct gcaataggca
     9301 acgacgtgtt ccttgccggg ttcgtccggc aggttcggct gcgtctggct atgggcgggc
     9361 acgatcgaac atagcaaagc agcggtgagc acaagcaaac atgatttttt tgccatggtt
     9421 cctccggaga aatgctgtca gacaactctt tccttcgggg ttaagatccg tttccgaggt
     9481 taccccgggc ttcgcccact aaacgccgcc caatcattgc ttcggcggct tgtgaaggtt
     9541 tctttccgat caaatgaccg cgttgagttg cctgtcgtat ttggtcccat tctttttgat
     9601 tgtgagtcta tccagcgtcg atattcttgc ggcagttttg aatctcccct cccacaacgt
     9661 gcccgatcga tcgtagatct cgttgatgta tcgaaaatat cttcgtccca cggtctgcat
     9721 taacaacgga gatctccgat ctcagcccgg ttccaccaaa gataaaaatg atttgtaata
     9781 acacgtaggc gtaaaatcct gcatcgaaat ctgtttttgc ccggcgaagg tatccaaaaa
     9841 gaacccgtaa tcatcaacgg caaagaaact agcagcgcga ttgtttccgc actggataat
     9901 ttgatgagga taagcaggga ttgtgattcg cggcgatcgt gccattcgtt atctcagtgc
     9961 cagcagattt tcctcggacc cctatcgccg gattactaca tcttcaagga aaagttctct
    10021 gacccatttt ggtttcaccg gccaaggccg cggtccaccg gcgtgctgag cgtcacagcg
    10081 acgcgtaaaa actcgctgtt cagactcttg atgtcatgct cccggccatc tccgtctgat
    10141 gtacggctga gcggccttac tacgatgacc agaaaacttt ttcttagagt gccacagagt
    10201 tcgcttcagc agatgtcgag acggcattga tatttcaaat agatggtcaa aaagcagcga
    10261 aacaaccacc gaatatgtaa tcggcccggc gtaacgggtc gggcgagcga gcgatcatcg
    10321 tcgcacgctc cgaagtgcac agacgaccgc atcaagcagc gttgtgcggt ctgcgccgcg
    10381 gagattgacc gtgcccgtga ttcccgcgcc acccgctggc cagaaatacg cgaacgcacc
    10441 ccaaactccg gtagcgccaa tcacctcctc accacacgcc aaattcacac cgattcccag
    10501 cccgtaacgg agataccgcg cggagctgtt gggcggccat cgcagcccgt cgcgccagga
    10561 ggtcagttcg gcagaccagc gttcgtcgaa aagcgttccg ccgaagagcc cacggagaaa
    10621 tctgataaga tccgccgcgg ttgtgacaag ccctccgcca gcccaatcaa acgaggtgtt
    10681 cataggtagc aaatcgcgct gtccatcata gtgatgagac acgtcggaac tccgtgacgg
    10741 ctcgtgccac tcgaggtaag tcgcgtccat gccgaggcgg gcgaagatca gcgatcggta
    10801 ggcatcggcg agagggcaac ccgcgagccc ctcgatggcg aggcccacta gcacgtagcc
    10861 ggtatctcca taggaaaagt tagtcccagg cagaaaggcg agccgattcc cctcgatcgc
    10921 ggcctcgacc aactccgccg ggcgccatat ccggtttgga tcggcgtgta cccgcgcttg
    10981 aaactgcgca cggaaaaaat aatcgggcaa cccactcgtg tggctcagca gccggcggat
    11041 ggtgagagcg tcgaggtctt cgagcccttc caggtcgcga agcagctcga ccaggtccgg
    11101 cggaaggaac ggtgtgaccg gactgtccag atcccagcgg cgttccgctg cgagacaaac
    11161 cgcggtcgcc gcggtcacgg ccttggtcac gcttgctgct cgaaatggat cgtttggtcg
    11221 cagcggccga gattgccctc tggcgaacag ccccctggcg ctggagaagg caatccccag
    11281 cgatggcgca tcgatcgcga tgattgcacc cggtgaaccg gctgcaacac acgcatcgag
    11341 cgccgccttt attcgcggtg caattatggc ctgtgatgtc acggttgagc caggtcgcgc
    11401 tattcagcgc tggtcaagat tgcgatgagc cgccgcatcg ctatatcaag ggctaacatc
    11461 gtcatgacca aattttcttt tcgacaaacg ctgccggcgt gggcgatata ctggatatta
    11521 gccctgctgt atatcacggc tgcgcaggca ttcacagaca tccgtcacgc ctatccgcgg
    11581 acttgaaacc tcttgccgcc gctattcaat acgtgcgtat acactcgact atcgcccaaa
    11641 gttccagaac ggtcattata tcttggccgg gcgaagacat tctaaaagaa ccgctagtca
    11701 tcatcggcaa agaaagtagc agcgcaattg tttccgcgct gactaataag cgagattgtg
    11761 attcgccgtg atcatgcgat ccgttatccc tgtgtcagca ggtttcgtgg cgcagcctct
    11821 cg
//
LOCUS       DASUTG010000930         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1180653, whole genome shotgun sequence.
ACCESSION   DASUTG010000930 DASUTG010000000
VERSION     DASUTG010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180653"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF089_21670"
     CDS             <1..523
                     /locus_tag="VF089_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176625.1"
                     /translation="LLLAQFERGASRDAYRELIEVSKKAVKNNGLHEWHTLQGEGKGS
                     RDYAGSAGVLSNAISQGLFGVYLNHGALTLKVRLAERTGSIRLHEPATDRQVSYQYCY
                     EPSPRLIRVRYSSNFPNAGRIHILLPQNKVPDTVLVDDRTSTFETESVGNERYVVLGT
                     DWSGHTLQIRLQN"
     gene            532..1332
                     /locus_tag="VF089_21675"
     CDS             532..1332
                     /locus_tag="VF089_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6176626.1"
                     /translation="MPTEIENSQYLSVVMPAFNEEGSVAAVVQKVIALPFVLEVVIVD
                     DCSTDRTAEIAERLATAHNQVKLLRHERNRGKTAALRTGIAATRGDIVIVQDADLEYD
                     PSEIAGVIRPILDGHADVVFGSRFLVRRATRVLYFYHYLANKFLTFISNLLTNLNMTD
                     IETGYKAFRGDIIRNMVIVSSGFGFEVEATAKVAKLGCPIYEVPISYYGRTYDEGKKI
                     GLTDGLLALWLVLRFNLFCSLSSSYRGLPVTNLPLAHERATLPKEKTS"
     gene            1351..2097
                     /locus_tag="VF089_21680"
     CDS             1351..2097
                     /locus_tag="VF089_21680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6176627.1"
                     /translation="MDPHNMGETIDYSGRDLEAMSWALNYHRWILQKFQPWLGRKVVE
                     VGAGTGSFSELLLESDLDSLTLVEPSPKMFAQLTERLSKTSTQIPITTYPSTFRKAAQ
                     GIRSAQHPDSIIYVNVLEHIDDDEAELRAVNETLERNGRLFIFVPALSWLFGPFDKQI
                     GHQRRYAKKELTEKCTSAGFRVIQAEYFDFFGIAPWWIKYRLLKSDQLEHSAVKIYDD
                     YVVPIARVIENTVVPPVGKNLILIAEKAQD"
     gene            complement(2094..>3199)
                     /locus_tag="VF089_21685"
     CDS             complement(2094..>3199)
                     /locus_tag="VF089_21685"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX6176628.1"
                     /translation="NESFSFVTLEAMAQQTPVLASSGSEVLVDHMVQSGAGETYNDYQ
                     SFAQALSGMLGDEYKSAEMGKRGREYVTSHFDFDRIRASLLNVIAENELITQPTSSAL
                     RISMSADQRLTLVTGDHGFTVKLEKHALTDMSGEEFYARPDTYDARIFEDVAVNNEYH
                     LPEQFEDSDIVIDIGAHIGSFSFAVLARGAGKVYAYESHPINFAITFQNLVRFGERAV
                     CNNLAVWRSDEPDQTLFNDLLNGPYQPNTGGISVLWNNEGLAVQTVALDQILIAASDG
                     LSKPIRLMKLDCEGSEYPILFTSKHLTIVEEICGEYHNINPEIVPDRALMGNTRDQFN
                     GNGLKMFLEAQGYAVEIIPQGIADGIFHARRLD"
BASE COUNT          798 a          801 c          821 g          779 t
ORIGIN      
        1 gctcttgctg gctcagtttg aaaggggcgc atcccgagac gcgtatcgcg agctgattga
       61 agttagtaag aaggcagtta agaataacgg tcttcacgag tggcacacgc tgcagggcga
      121 ggggaaggga agtcgcgact atgccgggag tgccggagta ctcagcaacg ccatttccca
      181 ggggctcttc ggcgtctatt tgaaccacgg agcactaaca ctgaaagtaa gattggctga
      241 gcggactggc agtattcgcc tgcacgaacc ggcgacagac cgccaagtct cttatcagta
      301 ttgttatgaa ccaagtccac gcctgatacg agtcagatac tccagtaact ttcccaatgc
      361 cggaagaatt catatattgc ttcctcaaaa caaggtgccc gacacggttc tggtagatga
      421 tcgcacgtca acttttgaga ctgagagtgt cgggaacgaa cgttacgtag tgttagggac
      481 ggactggagc ggccacactc tacagatacg acttcagaat tagtcacctg aatgccaaca
      541 gagatcgaaa actcacaata cctttccgtg gtcatgccgg cctttaacga ggagggcagc
      601 gtcgcggccg tggttcaaaa agtaattgct cttccattcg tgttggaagt cgtgatcgtc
      661 gacgattgct cgaccgaccg taccgcagag atcgcagagc gcctggcaac cgcccataac
      721 caggtgaagt tactcaggca cgagcgaaac cgtggaaaga ccgcagcgtt gaggactgga
      781 atagcggcca ctcgcggaga catcgtaata gtgcaggatg cagatcttga gtatgaccct
      841 tcggagatag ccggagttat ccgtcctatt cttgatggac atgctgacgt tgttttcgga
      901 tcacgcttcc tggttagaag ggccactcgc gtactctatt tttatcacta tctcgcgaac
      961 aagttcttga cgttcatttc gaatcttttg acgaacttga acatgacaga catcgaaacc
     1021 ggctataagg cgttccgagg cgacatcatc cgcaatatgg tgatcgtttc cagcggcttt
     1081 ggttttgaag ttgaagcgac tgcgaaagta gctaagctcg gttgtcctat ttatgaggtt
     1141 ccaattagct actacggaag gacgtacgac gaaggtaaga agattggatt gacggacgga
     1201 ctgctagctt tgtggctggt gctccgtttc aacttgtttt gtagcctatc ctcatcctac
     1261 cgtgggttac ccgtaacaaa tctgcctctg gcgcatgaac gggccacgct gccgaaggaa
     1321 aaaacgagct gagcatcgga gccatttcca ttggatccac acaatatggg cgagacgatc
     1381 gattattccg gaagagacct ggaagcgatg tcctgggccc tgaactacca ccgctggata
     1441 ttgcagaagt tccaaccttg gctgggccga aaagtagtag aagtcggagc tggaacaggt
     1501 tccttttctg agctgctgtt agaaagtgac ttagattcgc tgacacttgt tgagccttcg
     1561 cccaaaatgt ttgctcagct aacagagcgg ctaagcaaaa ccagcacaca aattcccatc
     1621 accacttatc cgtcaacatt cagaaaggcc gcgcagggga tacgctccgc tcaacatcca
     1681 gattcgataa tctacgtaaa tgttcttgaa cacattgacg atgatgaggc tgagctgcgg
     1741 gccgtgaatg aaacgctcga gcgtaacggt cgcctattca tcttcgttcc cgcccttagt
     1801 tggctatttg gaccttttga taaacagata ggtcatcagc gccggtatgc gaaaaaggaa
     1861 ctgactgaga agtgcacatc cgcgggtttc agagtgatcc aggcggagta tttcgatttt
     1921 ttcgggatcg cgccgtggtg gatcaaatac cggctcttga aatcggacca actggagcac
     1981 tcagccgtga agatttacga cgactatgtg gtccccattg cccgagtgat tgagaacact
     2041 gtggtgccgc cagttggtaa gaacttgata ctgattgcgg agaaagcaca ggactaatcc
     2101 agtcggcgtg cgtggaaaat tccgtcggct atgccctgag ggatgatttc gaccgcatat
     2161 ccttgagctt cgagaaacat cttgaggcca ttcccgttga actggtcccg agtatttccc
     2221 atcagcgccc ggtcaggcac aatctcagga ttgatgttgt gatactcgcc gcagatctcc
     2281 tccacgatgg tcagatgttt agacgtgaag agaatcggat attccgatcc ctcacaatcg
     2341 agtttcatta aacgtatcgg cttgctgaga ccgtccgaag cggcaatcaa gatctgatcc
     2401 agtgccactg tttgcacagc caatccttca ttgttccaca acactgaaat tccgcctgtg
     2461 ttcggctgat atggaccatt caagagatcg ttgaagagcg tttggtcagg ctcgtccgaa
     2521 cgccagacgg ccaaattgtt gcatactgct ctctccccaa atctcaccag gttttgaaag
     2581 gtgatcgcaa aattgatcgg gtgggattcg taagcataaa ccttgcctgc gcctctcgct
     2641 aaaactgcaa acgaaaaaga accaatatgc gcaccgatat caatgacgat atcggaatcc
     2701 tcaaactgtt ccggcaagtg gtattcgttg ttgaccgcta catcttcaaa gatacgagca
     2761 tcgtacgtgt ctggccgggc atagaactct tctccggaca tatcggtcag ggcatgcttt
     2821 tccagtttga ctgtaaagcc gtgatcgcca gttaccaggg ttaagcgttg atcagcggac
     2881 atcgagatcc tgagagctga cgaggttggc tgagtgatca attcgttttc ggctattaca
     2941 ttaagcagcg aggcgcgaat ccggtcgaaa tcaaagtggg acgttacgta ttctcttcct
     3001 cgcttaccca tctccgctga tttgtactca tctccaagca tcccacttag cgcctgggcg
     3061 aaactctggt agtcgttata agtttcgcct gcgccgcttt gtaccatatg gtcgaccaac
     3121 acttccgagc cgctactcgc aagcaccggc gtttgttggg ccattgcttc cagggtcaca
     3181 aaactaaagc tctcattct
//
LOCUS       DASUTG010000931        12066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1036267, whole genome shotgun sequence.
ACCESSION   DASUTG010000931 DASUTG010000000
VERSION     DASUTG010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036267"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1632)
                     /locus_tag="VF089_21690"
     CDS             complement(85..1632)
                     /locus_tag="VF089_21690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6176629.1"
                     /translation="MSHLPGEITLLLLLPLIGALVLLLIPRQQRQTLFTLALLSSIAA
                     FIWSLRLFQAFDAGAGEMQFVERIPWMPAFGIGYIVGIDGISLFLVLLTTFLMPLAIA
                     ASWPVQDKIKEYLFFMLVLETGMLGAFVALDLFLFYVFWEVMLVPMYFLIGVWGGTRR
                     IYAALKFVVYTMAGSLPMLVAIIYLASRYAQVNQTMTFDLLQLYDLRLPVNEQIWLFL
                     AFGLSFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFAMPLFPDAA
                     LVLTPVIIALAVIGIIYGAGVAMMQPDLKKLVAYSSVSHLGFVMLGLFALNIQGIQGG
                     IYQMVSHGLSTGALFLLVGMLYDRRHTRMIADFGGLWQRLPVLSVMFLIVTFASIGLP
                     GLNGFIGEFLILLGAFSVTPGWASAAATGIILGAIYMLWMFRRVFFGPMTHPENQKLT
                     DLNQRELLILAPVVALIIIMGIYPQPFLSRMKPAVDITLHRILGSQAAPVAANHATVG
                     EMNSDGR"
     gene            complement(1635..3557)
                     /gene="nuoL"
                     /locus_tag="VF089_21695"
     CDS             complement(1635..3557)
                     /gene="nuoL"
                     /locus_tag="VF089_21695"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6176630.1"
                     /translation="MVASTATVAALDLLRWIPLIPLGAGVFNVFFGRRLGGKTAGGIA
                     SAAVGISFALALYVFWRLPATGIVRDLWYTWIQSGSFQVNIAFQVDALSAVMLLIVTG
                     IGFLIHIYSLGYMADDDGMARFFIYLNLFIFFMLLLVMADNVLLLFIGWEGVGLCSYL
                     LIGFWYQDAKNVIAGNKAFILNRIGDFGFLLGIFLLVTELGRQGIWTLDFAELQKHVQ
                     MITPAKITLITVLLSVGAAGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVYMI
                     ARLHFLFILAPYTLPIIAVVGAATAFFAATVALAQNDIKKVLAYSTVSQLGYMFLAVG
                     IGAFSAAIFHLFTHAFFKACLFLGSGSVIHALQGEQDMRKMGGLKKALPQTYWTYLIA
                     TLAIAGIPFTAGFFSKDLILWRAFEDASYGLWAIGIITAGLTAFYMFRQLFMVFHGSL
                     RADDHIKAHIHESPPVMTVPLVILAVGSIFSGWLGAPEYLWGSLWDDWLQPVFGTGHS
                     VEHAPVTTELLVTSMTMAVVALGIYLAYARYGRAESTVPAAGATGLYGLLLESYRIDD
                     VYDRLVVRPFTAASDWLARVFDPAVIDGLINGVAKVTRGLSLIWRESQTGNIQHYLIG
                     FLAGTLALLFYYLRQQ"
     gene            complement(3551..3853)
                     /gene="nuoK"
                     /locus_tag="VF089_21700"
     CDS             complement(3551..3853)
                     /gene="nuoK"
                     /locus_tag="VF089_21700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6176631.1"
                     /translation="MVPLEYYLVLSAIIFAIGVIGVLIRRNLIVVLMSIELMLNAVNL
                     TFVAFSNSLGSMDGQVIVFFVMAVAAAEAVVGLAIIISVFRHRQSLDPQEMQLLKW"
     gene            complement(3860..4354)
                     /locus_tag="VF089_21705"
     CDS             complement(3860..4354)
                     /locus_tag="VF089_21705"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6176632.1"
                     /translation="MSGPQILFFIVAALLIVSSILVVFQRNVVHSAVALVAALFLIAV
                     LFLSLNAPMVGVLQILVYAGAIMVLFLFVIMLLNPVGLEQRRSLWWGAGGISGLLLVA
                     ALAPVLFNIESAQDPVVATELFGGPEELARSLFTDFVLPFEIASVLLLVAIIGAVVLA
                     NKEP"
     gene            complement(4359..5498)
                     /locus_tag="VF089_21710"
     CDS             complement(4359..5498)
                     /locus_tag="VF089_21710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6176633.1"
                     /translation="MIVDIGIVAIKAFLVLFMVLNLAGVLGWVERKGSALIQDRIGAN
                     RASIFGFAGMGLINTLVADPLKFLTKEDFIPPAGDRFLHTLAPCLALFPALVTFAVIP
                     FGDVLQIGERVINLQVANLNVGILYIFAMTSLGVYGLVIGAWASNNKFSLLGGVRGSA
                     QMISYEVALGLSVIGILMVYGTLDLQEIARGQGTLIRDLLPESWAVLKAAVGWLPAWG
                     IFLQPLAFLLFFTAAIAETRRIPFDLPEAESELVAGSHVEYSGGKFLMFFAAEFAEIV
                     TVAALITTLFFGGWQVPYLTRDGFHFPWGGTLLLPHLAVVLLQVGAFTFKVILFCWLQ
                     ILLRWSVPRFRYDQVMRLGWKMLLPAALVNVMITAFVILFMQRIP"
     gene            complement(5495..7084)
                     /locus_tag="VF089_21715"
     CDS             complement(5495..7084)
                     /locus_tag="VF089_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6176634.1"
                     /translation="MDPVKLTIDGQEISTVKGKTVIQVAAEAGIAIPHYCYHPKLSIA
                     GNCRMCLVEIEKMPKLQIACNTQVAEGMTVLTQSPKVVSVRKAVMEFLLINHPIDCPI
                     CDQAGECWLQDYYMKHDLQDSRFKERKERDRKREIFGPNVVFDGERCIKCTRCVRFLD
                     EITHTKELTVDNRSDDSTIALFPGTVLDNPLSANVVDICPVGALTDRDFRFKVRVWYL
                     QKTPSICPGCSTGCNISVEHYQNRIARFKPRVNEAVNSHWLCDEGRYCFYDLTGGERL
                     TTPMIRQEGGLVPTTWETALRAVVSGLRSAGSLAGIISGRNTNEEAFLFAKLMKKLSP
                     AATLEVFYQERELTEVEKILVSPDRSPNFRGAAEMGVGSNGGFEPLMHKLLAGNYGGA
                     YVVGEDLTAANGDQAELRGALGKLSFLVVQDIRLTETAKAAHVVLPSTHFGEKEGTYT
                     NRKGRVQKLNAALVAPDGALQDCEIFIRLLEAAGERASYSTAEQVFQALSQEIPSYRG
                     LNYDVIGDQGIQLGSGGADRS"
     gene            complement(7163..8440)
                     /gene="nuoF"
                     /locus_tag="VF089_21720"
     CDS             complement(7163..8440)
                     /gene="nuoF"
                     /locus_tag="VF089_21720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX6176635.1"
                     /translation="MEKILLRNVDAADSHTLAAYQARGGYRAWEKVVRSMKPEQLIDE
                     VKASGLRGRGGAGFPTGMKWSFVPKDSPKPKYLICNADESEPGTFKDRLLIEKDPHAI
                     IEGTLIAAFGIQSHTAFIYIRGEMAFGTRVLEQAAEEVKRAGLTGKNIFGSGYDLDLI
                     VHRGAGAYICGEETALLSSLEGGRGWPKVKPPFPATHGLFGCPTVVNNVETLAALPWI
                     IDHGAAAYAALGTEKSKGTKLFSVSGHISKPGVYEVEMGYPFKKFLEEDCGGVPNGRK
                     LKGVIPGGGSMPVLRPEEIENVNLDYESVQAAGSLLGSGGVIVMDDSTCMVRAAWNIS
                     RFYAHESCGQCSPCREGCHWMEKIFRRIEHGQGDRGDMDLILNVSGNIMGNTICPFGD
                     AAAMPAAAFIKKYRDEFEQHIVARGCTVRGKIS"
     gene            complement(8458..8931)
                     /gene="nuoE"
                     /locus_tag="VF089_21725"
     CDS             complement(8458..8931)
                     /gene="nuoE"
                     /locus_tag="VF089_21725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX6176636.1"
                     /translation="MPVEFSPETLKKFEETVSRYPKKEAAMLPVLYRAQQEFGYLSPE
                     AIDYVAKLMGQSPAKVHGVVSFYTMYNMKPIGRHHIQVCRTLSCALGGAEKITDFIKK
                     KLAIELGQTTDDGRFTLSEVECLASCGTAPMMQINDDYYENLDEEKVTEILDSLK"
     gene            complement(9118..10320)
                     /locus_tag="VF089_21730"
     CDS             complement(9118..10320)
                     /locus_tag="VF089_21730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX6176637.1"
                     /translation="MSVPEKPDSQNLPAEIMELQMGPSHPATHGTIKFNLKLEGEKVL
                     DCDVEVGYLHRGFAKSCESVTWTQVIPYTDRLNYASPLINNVGYAMAVEKLIGVETPP
                     RCQYIRVIASELSRIYDHLTCCGMCCSELGAITVGFYMIEAREMIARITEALTGARLT
                     VTYVRVGGVKHDLPLDFAERVERTFKIIRKLLDDCDGLLSRNRIFIDRMSNIGVISRE
                     TAISYSLTGPILRATGVNYDVRKTNPYLVYDQLDFMVPLGTKGDNYDRFVCRIQEIEQ
                     SMKIVEQALKNLPVGPVWIEDPRFVLPEKEKVYNSIEGLMHHFKIIMEGVKVPPGETY
                     FAVEGGNGELGFYLVSDGAGRPYRVRCRPPCFLAMGAFHEMIKGYMVADIVPTFGMVN
                     MIGGECDR"
     gene            complement(10322..10885)
                     /locus_tag="VF089_21735"
     CDS             complement(10322..10885)
                     /locus_tag="VF089_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6176638.1"
                     /translation="MPETSPILQDIQEKLGSKVLETSQFRGDDIVVLDRVDLRESFRI
                     LKEDPGLRFDFLSDITAVDYWKKKEPRFELVYQLLCLQNGRRLRVRVPVSEENAAIES
                     LIPLWRGANFLEREIWDLFGIKFIGHPDLRRILLFDEFKGHPLRKDYPINQQQHRVPE
                     RKVEGTFVDERSHNKLLRLKKSLGSKD"
     gene            complement(10892..11248)
                     /gene="ndhC"
                     /locus_tag="VF089_21740"
     CDS             complement(10892..11248)
                     /gene="ndhC"
                     /locus_tag="VF089_21740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6176639.1"
                     /translation="MDHPYFPLVIVFALAAFVVLALLFIAQKVGPKSTNPAKMEPFES
                     GNPPKGDARIRFSVRFYLVAMLFLIFDLEVVFLYPWAIYFRQLGLFGLVQMGIFLTIL
                     TIGYVYVWKKGALEWD"
     gene            complement(11383..>12066)
                     /locus_tag="VF089_21745"
     CDS             complement(11383..>12066)
                     /locus_tag="VF089_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX6176640.1"
                     /translation="LNWIYQVLRKPGEILAPVSASLAKSPDSTWRNYGSLFEKHSTNI
                     ISPEFLAALAQVEGSGNPIASTYWRWRWSWNPFDIYRPASSALGMFQITDGTFAEARN
                     YCIRDHQVVSAGPWHDVHSCWFNRFYSRTVPSHSIQLTAAYLHRTVVHILAARPKVRS
                     TPGQQQKLAAVIHLCGSKRGEIFARRGFKLASGERCGTHNVARYLARIDVMKRRFVAL
                     RMSGSRSTD"
BASE COUNT         3096 a         3380 c         3181 g         2409 t
ORIGIN      
        1 tcatgctcgg cgaaaaatag gtccagcagc atgaccacta aagctgtcag caagatttgc
       61 gctgccggca aaagaggtac gagattaacg tccatcgctg ttcatctctc cgacggtggc
      121 gtggttggca gcgacgggcg cagcttgcga gccaaggatt ctgtgcagag ttatatcgac
      181 agcaggcttc atccggctga gaaagggttg cggataaatc cccatgatga tgatcagcgc
      241 gacaaccggc gcaagaataa gcagctcgcg ttgatttaga tcggtaagct tctggttttc
      301 cggatgagtc atcggtccga aaaatacccg ccgaaacatc cagagcatat aaatggcgcc
      361 aagaatgatg ccggtggccg cagcggaggc ccacccgggc gtgacgctga aagctcccag
      421 tagaattaaa aactcaccaa tgaagccgtt caatccgggc aagccaatgg atgcgaatgt
      481 gacgatcagg aacatcacgg ataataccgg caggcgctgc cacagtccgc cgaaatcggc
      541 gatcatgcgc gtatgacgcc ggtcgtagag catgccgact aaaagaaaca gcgctcctgt
      601 ggacaggccg tgactcacca tctgataaat cccgccctga atgccttgga tattgagagc
      661 gaacagtccg agcatgacaa aacctaagtg gctgaccgag gagtaagcca ccagtttctt
      721 caagtccggt tgcatcattg ccacgccggc gccatagatg atgccgatga ccgccagcgc
      781 gatgatcacc ggcgtcagaa ccaacgccgc gtcggggaat aacggcatcg caaagcgaag
      841 aaacccgtag gttcccatct tcagcaagac acccgcgagg atcaccgagc ccgccgtcgg
      901 cgcctcgacg tgagcatcgg gaagccaggt gtgaaacgga aagagcggca ctttgatggc
      961 aaacgacaag ccgaaggcga ggaacagcca gatttgctcg ttgacaggta agcgcaaatc
     1021 gtaaagctgc aacagatcga aagtcatcgt ctgattcacc tgggcataac ggctggccag
     1081 gtaaataatc gccaccagca tgggcaagct gccggccatg gtgtaaacaa caaacttcag
     1141 cgcggcataa atccgccgag tgccgcccca tacgccgatg agaaagtaca tcggcaccag
     1201 catcacttcc caaaacacat aaaaaagaaa cagatcgagc gctacgaatg cgcccagcat
     1261 gccggtttcc aagaccagca tgaagaaaag gtactcttta attttgtcct gcaccggcca
     1321 ggaggcggca atcgccaacg gcatgagaaa ggtcgtcagc aaaactagaa aaagacttat
     1381 gccgtcgatg ccgacgatat agccgatgcc gaaggccggc atccaaggga ttctttcaac
     1441 gaactgcatt tcgcccgcgc cggcgtcgaa agcctgaaac aggcgcaacg accagataaa
     1501 ggcagcgata ctcgaaagca gagctagagt gaacagggtt tgccgctgct gacggggaat
     1561 cagcagtagc accaaggcgc caatcagagg cagtaggagg agtaaagtaa tttcgcccgg
     1621 caagtgtgac atgattattg ttgtcttaga taataaaaaa gaagcgccaa ggtccccgcg
     1681 agaaagccga ttagatagtg ctggatatta ccggtctgag actcacgcca gataaggctc
     1741 aaacctcgtg tcactttggc cacgccgttg atcaagccgt cgatcacggc aggatcaaaa
     1801 actcgagcca accaatccga tgcggccgta aacggccgga caaccagccg atcataaacg
     1861 tcgtcaatcc gataactctc aagcagcaat ccgtagagcc ctgtggcacc cgcagcaggc
     1921 acggtgcttt cggcgcgacc gtatctggcg taagccaggt agatgcccag cgcaaccacg
     1981 gccatagtca tggaagtcac caggagttcc gtcgtaaccg gagcatgctc cacggaatga
     2041 ccggtgccga atacgggttg gagccagtcg tcccaaaggc tgccccataa atattccggg
     2101 gcgcccaacc agccgctaaa gatcgagccc acggcgagga tgaccagagg cacggtcatg
     2161 acgggcggcg attcatggat atgcgctttg atatgatcgt cggctcttaa actgccatgg
     2221 aaaaccatga acagttgccg gaacatatag aacgcagtca ggccggcagt gatgatgcca
     2281 atggcccata agccgtacga tgcatcttca aacgctctcc agagaatcag gtccttggaa
     2341 aaaaacccgg ccgtaaacgg tatcccagct atcgccagtg tggcgatcaa gtaggtccaa
     2401 taagtttgcg gcaaggcctt tttcaaaccg cccatcttgc gcatgtcctg ttctccctgc
     2461 agcgcgtgaa tcacactgcc ggaacctaaa aacaaacacg ctttgaagaa agcatgggtg
     2521 aacaaatgga aaattgccgc gctaaatgcg ccgatgccca ccgccaaaaa catgtaaccg
     2581 agttgactaa cggtggaata ggcaagaact tttttgatgt cattttgcgc cagcgccacg
     2641 gttgcggcga agaaggctgt ggctgcgcca accacggcaa tgatcggtag ggtataaggc
     2701 gccaggataa acaagaagtg caggcgagcg atcatgtaga ccccggcggt taccatggtt
     2761 gccgcatgaa tcagcgcgct aaccggagtc ggacccgcca tggcatcggg gagccagaca
     2821 aacagcggga tctgcgccga tttgccggcg gcgccgacag aaagaagcac ggtgatcaga
     2881 gtgatcttcg ccggcgtaat catctgaaca tgcttttgca gctcggcaaa atccagagtc
     2941 cagattccct gcctgcccaa ttccgtgacc aggaggaaaa tgcccaggag gaaaccgaag
     3001 tcgccgatgc gattaaggat aaatgctttg ttgccggcga ttacgttttt cgcgtcttga
     3061 taccaaaaac caatcagcag ataggaacac aagcccacgc cttcccaacc gataaacaag
     3121 agcagcacat tgtcagccat gaccagcagc aacatgaaaa aaataaaaag gtttaaataa
     3181 atgaagaagc gcgccatgcc gtcgtcatcg gccatgtaac ccaaggaata aatatggata
     3241 aggaaaccga tgccggtgac gatcaagagc atcactgccg atagggcgtc gacctgaaac
     3301 gcgatattga cctggaagga acctgactgg atccaggtat accaaagatc gcggacaata
     3361 cctgtggccg ggagccgcca aaagacataa agcgccagcg cgaaagaaat cccgaccgca
     3421 gctgaagcga tgcccccggc tgtcttgccg cccaaccttc gcccgaaaaa gacattgaac
     3481 acgccggcgc ccagcgggat cagcggaatc cagcgcagca aatccaaagc ggcaacggtg
     3541 gcggtggagg ctaccattta agaagctgca tctcctgagg atcgagtgat tggcgatggc
     3601 gaaacaccga gataatgatg gcaagtccca ccactgcttc ggcagcagcc acggccatga
     3661 caaagaacac aatcacctgc ccgtccatgg agcctagcga gttggaaaaa gcgacaaagg
     3721 tcagattcac cgcattgagc ataagctcga tggacatcag cacgacaatc aggttgcggc
     3781 ggatgagaac gccgatgaca ccgatggcaa atatgatcgc gctcaaaacc aggtaatatt
     3841 ccagcggcac catagacgct tacggctcct tgttcgccaa aaccacggcg ccaatgattg
     3901 ccaccagaag caacactgag gcgatttcga aaggaagaac aaagtccgta aaaaggctcc
     3961 tggccagctc ttccgggccg ccaaaaagct ccgtggcgac tactggatcc tgggcagact
     4021 cgatattgaa caaaaccgga gccaacgccg cgaccagaag caagcctgaa atgccgccag
     4081 cgccccacca aagactgcgg cgctgttcca gcccgacagg attcaaaagc attattacga
     4141 agagaaagag caccatgatg gcgccggcat agacgaggat ctgcaacacg ccaaccatgg
     4201 gcgcgttcaa gctcaggaag agaacagcaa taaggaacaa cgctgccact aaggcgaccg
     4261 cgctatgaac cacattgcgc tgaaacacta ctaatatcga agatacgatc agcaaagccg
     4321 caacgatgaa gaaaaggatc tgtggaccac tcatagtctt acgggatgcg ttgcatgaat
     4381 agaattacaa aagcggtgat catcacgttc accagcgccg ccggcaggag catcttccag
     4441 cctaaccgca tcacctggtc gtaacgaaat ctaggaacgc tccagcgcag cagaatctgc
     4501 agccagcaaa acaggatgac cttgaaagta aacgcgccga cctgcaacag gacaaccgcc
     4561 aagtggggca gaagtagggt accgccccaa ggaaagtgaa aaccatcccg cgtgaggtag
     4621 ggcacctgcc agccgccgaa gaagagcgtc gttatcaacg cggcaacggt gacgatctcc
     4681 gcgaattcag cggcaaagaa catcaggaac ttgccgcccg agtattcaac gtgagaaccg
     4741 gcaactaatt cggactctgc ttccggtaaa tcgaacggta tccgcctggt ttcagcaata
     4801 gccgcggtaa agaacagtaa aaacgccagc ggctgcagga aaatccccca ggccggcagc
     4861 caacccacgg cagccttgag aaccgcccac gactccggaa gcaaatctct gatcagagtg
     4921 ccttggccgc gcgcgatctc ctgaaggtcc agggtaccgt aaaccataag aatgccgatg
     4981 accgaaagac cgagcgccac ttcgtaagaa atcatttgcg ccgaaccgcg gacgccgccc
     5041 aagagagaga atttgttgtt cgatgcccag gcgccgatca caagaccgta aacgcccaaa
     5101 gaggtcatgg caaagatgta aaggatgccg acgttgagat tagcaacttg aagattgatc
     5161 acccgctcgc caatctgaag aacgtcgccg aaggggatga ccgcaaaagt taccagggcc
     5221 gggaataaag ccaggcacgg cgcaagggta tgcaggaacc ggtcaccagc cggcggtatg
     5281 aagtcctctt tggttagaaa tttcagcggg tcggcaacca gggtattgat cagccccata
     5341 ccggcaaagc cgaaaatgga cgcccgatta gcgccgatgc gatcttgaat cagtgcgctg
     5401 cccttgcgct caacccagcc gagcactccg gccaggttga gaaccatgaa aagcaccagg
     5461 aaggctttga tcgcgacgat gccgatatca acgatcatga ccggtccgca ccaccactcc
     5521 ccaactgaat tccctgatcg ccgatgacat cgtaattaag tcctctatag gaaggaattt
     5581 cctgtgacaa ggcctgaaag acttgttccg ctgtcgagta agatgctctc tcaccggccg
     5641 cctccagcaa gcgaatgaag atctcacagt cctgtagagc gccgtccggc gccaccagcg
     5701 cggcattaag cttttgcacg cgacccttcc ggttcgtgta agtgccctct ttttcgccga
     5761 aatgagtgga gggcaaaacc acatgggcag cctttgccgt ttcggtcaag cggatatcct
     5821 gcactaccag gaaagataat ttgcccaagg caccgcgcag ttccgcttgg tcgccgttag
     5881 cggccgttag atcctcgccc actacgtagg cgccgccata atttcccgcg agcagcttgt
     5941 gcatcaaagg ctcgaagccg ccattggagc cgacccccat ctcggcggcg ccgcggaaat
     6001 taggcgaacg atccgggctc acaaggatct tctctacttc ggtcagctcg cgctcttgat
     6061 agaacacttc cagagtcgca gcgggcgaaa gcttcttcat cagcttggcg aacaaaaagg
     6121 cttcttcgtt ggtgtttctg ccggaaatta ttcccgccag cgaacccgcc gagcgcaggc
     6181 cactgaccac cgcgcgcagg gctgtctccc aggtagtcgg aacaagcccg ccctcctggc
     6241 gaatcatggg tgtggtcaac cgctcaccgc cggtcaaatc atagaaacag tatcggccct
     6301 catcgcagag ccagtggctg tttaccgctt cgttcacccg cggcttaaaa cgagcaatgc
     6361 gattctgata atgttcgacg ctgatattgc acccggtgct gcaccccgga cagatcgagg
     6421 gggttttctg caggtaccag accctgacct tgaaacggaa atcacgatcg gtaagcgcac
     6481 cgacggggca gatatccacc acattggccg acagcgggtt gtcgagcacg gtgccgggaa
     6541 atagcgcgat cgtcgaatca tcgctgcggt tatcgacggt cagttccttg gtatgggtta
     6601 tttcgtcaag aaagcgcacg cagcgggtgc atttgatgca acgctcacca tcgaaaacga
     6661 cgttgggacc gaaaatctcg cgcttacgat cacgctcttt tcgctccttg aaccggctgt
     6721 cctgcaggtc atgcttcatg taatagtcct gcagccagca ctcacccgcc tgatcgcaga
     6781 tcggacaatc gatcggatgg ttgatcagaa gaaattccat cacggccttg cgcaccgata
     6841 ctaccttggg gctctgcgtc agtaccgtca tgccctcggc cacttgtgtg ttgcaggcga
     6901 tctgcagctt gggcatcttc tcgatttcga ccaaacacat gcggcaattg ccggcgatcg
     6961 aaagcttggg atgatagcag taatgcggaa tcgcaatccc cgcctcggcc gccacctgaa
     7021 tgacggtctt gcctttgacg gtgctgatct cttggccgtc gatagtgagt tttactgggt
     7081 ccataaaata aagctggtca atcgagtgtt ggagtactgg actattggct cctcttaccc
     7141 atcgctccaa tactccaata cttcaactga tctttcccct tacggtgcaa cctctcgcga
     7201 cgatgtgctg ctcaaattca tcccgatatt tctttataaa ggctgcggcg ggcatggcgg
     7261 cggcatcgcc aaagggacag atcgtattac ccatgatatt gccgctgacg ttgaggatga
     7321 gatccatatc gcctctgtcg ccctgcccgt gctcgatgcg gcggaagatc ttttccatcc
     7381 agtgacaacc ttcgcggcaa gggctgcatt ggccgcacga ttcatgagca tagaaacgtg
     7441 agatgttcca cgccgcgcgg accatgcaag tggagtcatc catgacgatg acgccgccgg
     7501 agccgagcaa actgcctgcc gcctggaccg actcataatc cagattcacg ttttcgatct
     7561 cttccggccg caagaccggc atcgatccgc caccgggaat cacgcctttg agttttctgc
     7621 cgttgggaac accgccgcaa tcctcttcaa ggaacttctt gaacggataa cccatctcga
     7681 cttcgtaaac gccgggcttg ctgatgtgtc cgctgacgct aaagagtttc gtgcctttgc
     7741 tcttttcagt ccccaacgcc gcataagccg cagcgccgtg atcgatgatc caaggcagcg
     7801 ccgccagtgt ttcgacgttg ttcaccactg ttggacagcc gaacaggcca tgggttgccg
     7861 ggaaaggcgg tttgaccttg ggccagccgc ggccgccttc cagcgaggaa agcaaagcgg
     7921 tttcttcgcc acagatatag gcgccggcgc cgcgatgaac gatcagatcc aggtcgtagc
     7981 ccgagccaaa gatattcttg ccggttaaac cggcgcgttt gacctcttca gccgcctgtt
     8041 ccagcacccg ggtgccgaaa gccatttcgc cgcggatgta aatgaaggcg gtatgcgatt
     8101 gaatcccgaa ggccgcaatc agcgttcctt caatgatcgc gtgtggatct ttctcgatca
     8161 gcaaccggtc cttgaatgtg ccgggttcgc tttcgtctgc gttacaaatt agatacttgg
     8221 gtttgggact gtctttgggc acgaagctcc acttcatgcc cgtcgggaat cccgcgccgc
     8281 cccggcccct gagcccggac gctttcactt cgtcgatcaa ttgctccggc ttcatgcttc
     8341 gcacgacctt ttcccaggcg cgataaccgc cgcgtgcctg gtaggccgcc agagtgtgcg
     8401 aatcggcggc atcgacgtta cgaagcagaa ttttttccat cggtctatca gataaagtta
     8461 cttcaaagaa tcgaggattt cagttacctt ttcctcgtcc aagttttcgt aatagtcatc
     8521 gttgatttgc atcatcggcg ccgtcccaca ggaagccaag cattcgactt ccgacaacgt
     8581 gaatcggccg tcatccgtcg tctggccgag ttcgatagcc agttttttct tgataaaatc
     8641 agtgatcttc tccgccccgc ccagggcgca tgacagcgtg cggcaaactt gaatatggtg
     8701 ccggccgatg ggcttcatgt tgtacatagt atagaagctc acgaccccgt gcacctttgc
     8761 cggcgattgc cccatcagct tggcaacata gtcaatcgcc tcgggactca gatagccaaa
     8821 ttcctgctgg gcacgataga gaaccggaag catcgccgcc tctttcttgg gatagcggct
     8881 caccgtctcc tcgaattttt tgagcgtctc cggcgaaaat tccacaggca tgcggttatt
     8941 cttctccgtt aaacaacaac tgctaacgcg ctgtctgaaa agtctgctag caggctgctc
     9001 aaaaagatct cagaggcgag gcgccgcgaa gttcgatgag ctgaggcgta cgcttcagta
     9061 cgttgaagcg agtcgggcga agtgcaacga agcatatgag tctttttcag cagcctgcta
     9121 ccggtcgcat tccccaccga tcatattgac catgccgaaa gtcggcacga tatcggcgac
     9181 catatatccc ttgatcatct catgaaaagc gcccatggcc agaaaacagg gcgggcggca
     9241 tcgcacccgg tacgggcgtc cggcaccgtc gctcaccaga taaaatccca attctccgtt
     9301 gccgccttcc accgcaaagt acgtttcacc cggcgggacc ttgactcctt ccatgatgat
     9361 tttgaaatgg tgcatcaatc cttcgatgct gttgtaaact ttttcttttt ccggcagcac
     9421 gaaacgggga tcctcaatcc acaccggtcc gacaggcaga ttcttcagag cctgctcgac
     9481 aattttcatg ctctgttcga tttcctgaat gcggcacaca aagcgatcat agttgtcgcc
     9541 tttggttccc agtggcacca tgaaatcgag ttggtcgtaa accagatagg gattggtttt
     9601 ccggacgtcg taattaaccc cggtcgcgcg caagatcgga ccggtcaggc tatagctgat
     9661 ggcggtctcg cgcgaaatca caccgatatt ggacatccga tcgatgaaga tacggttgcg
     9721 cgacaggagt ccgtcgcaat cgtccaacag cttgcgaatg atcttgaagg tgcgctctac
     9781 ccgctcggca aaatccagcg gcaaatcgtg tttgaccccg ccaacgcgga cgtaagtcac
     9841 ggttaagcgc gcgccggtga gagcctcagt tattctcgcg atcatttcgc gggcttcgat
     9901 catgtagaaa cctacggtga tcgcgcctag ctccgagcag cacatgccgc agcatgtaag
     9961 atgatcgtag atacgcgaca gctcgctggc gatcacgcga atatactggc agcggggcgg
    10021 cgtttcgacc ccgatcagtt tctctactgc catcgcgtaa ccgacattgt tgatcaacgg
    10081 cgaggcatag ttcaggcggt cggtataagg aatgacctgt gtccaggtga cgctctcgca
    10141 gcttttggca aatccacgat ggagatagcc aacctcgaca tcgcagtcga ggaccttctc
    10201 gccttccagc ttaagattga atttgatggt gccatgggtt gccggatgag atggccccat
    10261 ctgcagttcc atgatttctg ccggcagatt ttggctgtcg ggcttttccg gcactgacat
    10321 gttaatcttt gctcccgaga cttttcttca aacgcaaaag cttgttgtgt gaacgttcgt
    10381 cgacaaaggt tccttcgacc ttgcgctccg gaacccgatg ctgttgttga ttgatcgggt
    10441 agtccttgcg cagcggatga cccttgaatt catcaaacag caagatccgg cgcagatccg
    10501 gatgaccgat gaacttgatg ccaaacaaat cccaaatttc ccgctccaga aagttcgcgc
    10561 cgcgccaaag cgggattagc gattctatcg cggcgttttc ctcggatacc ggcacccgca
    10621 cccgcaatcg tctgccgttt tgcagacaca gcaactggta aacgagttcg aagcgtggtt
    10681 ctttcttttt ccagtagtca acggcggtga tgtcagaaag aaagtcaaaa cgtagcccag
    10741 gatcttcttt cagtatacga aaactctctc tcaaatccac ccggtcgaga accacgatgt
    10801 cgtcgccgcg gaattggcta gtctctagaa cctttgagcc gagcttttcc tggatgtcct
    10861 ggagaatggg gctggtctca ggcatcggcg atcaatccca ttccagcgcg ccttttttcc
    10921 aaacgtacac ataaccaata gtaagaatgg tgaggaaaat tcccatttgc actaatccaa
    10981 aaagccccag ctgccggaag tagatcgccc acggatagag aaacacaact tccagatcga
    11041 agattaaaaa cagcatagcg actagataaa accgcaccga aaaacgaata cgcgcatcgc
    11101 ctttgggcgg attgccggac tcaaacggtt ccatctttgc tgggttagtg gatttcggtc
    11161 cgaccttttg cgcaataaag agcagagcca gaaccacaaa tgccgccagc gcaaatacaa
    11221 tgaccagagg aaaataagga tgatccatca atgacagcta tctaaaaaaa tccataccta
    11281 accgtctcca gaggtatgga ttggtagaat gaatgctgaa ggtactaaaa atttctattg
    11341 gaaagttgcg gggttgtcaa gataacaagg cgcaagccgc aattaatcgg ttgatcggct
    11401 acccgacatt ctcaatgcaa caaagcgtcg cttcatgaca tcaattcgcg caagatatcg
    11461 cgccacattg tgagttccgc aacgctcgcc ggacgccaat ttgaagcccc gacgcgcgaa
    11521 gatctcaccc cttttcgatc cgcacaggtg aattacggca gccaatttct gctgttgacc
    11581 cggtgtgctc cgaaccttgg gccgagcggc caaaatgtga acgacagtgc ggtggagata
    11641 tgcggcggtt agttggattg aatgactcgg cacggtgcgg ctatagaagc ggttgaacca
    11701 gcaggagtgg acgtcatgcc agggtccagc ggagacgact tgatgatccc ggatgcagta
    11761 attacgcgct tcggcgaacg tgccgtcggt gatttgaaac atgccgagcg cgctggaagc
    11821 cggacgataa atatcgaaag gattccatga ccagcgccaa cgccagtaag tgctagcgat
    11881 cgggttgccg ctgccttcca cctgagccag cgcggcaaga aattcaggag aaataatgtt
    11941 cgtagaatgt ttctcgaaca gcgatccata attccgccaa gtagaatcgg gcgacttagc
    12001 taaggaggca ctgaccggcg ccaggatctc cccgggcttg cgaagcacct gatagatcca
    12061 gttgag
//
LOCUS       DASUTG010000932         6211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1626335, whole genome shotgun sequence.
ACCESSION   DASUTG010000932 DASUTG010000000
VERSION     DASUTG010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626335"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..592
                     /locus_tag="VF089_21750"
     CDS             23..592
                     /locus_tag="VF089_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176641.1"
                     /translation="MKRTKLIVVMAAVIFVAGPSGYLLAQHMQGGPMMQSPAQQKSPT
                     QESGESSQQRESPTGSEIVPPYGMGRGGGYGPGYGYGMGPGMMGPGMMGPGMMGPGMG
                     MAGGFGPGMGMMGPMMHSIMGSPQMMGLMMSMHGDMMAMMGRMIQHYGGEQSPEVQQK
                     MQTEMLQEMGDILVKHGNRLKESAKSSGR"
     gene            1149..2033
                     /locus_tag="VF089_21755"
     CDS             1149..2033
                     /locus_tag="VF089_21755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6176642.1"
                     /translation="MSLTSIVEKQLRGNFVIFHPSDFSAGSEVAFGHALKIAMQAKAK
                     LDIMHVVETHISPEKPDWQDFPAVRATLSRWGILPEGVGRDEVAKAGLHIRKILQAGA
                     DPLKTMLRHFRKLPPDLIVLATHQREGLERWLHKAVAEPLARRSGAMTLFVPSEAKGF
                     ISVKDGSVRLKKILIPIDHDPDPQTALSKASLLAQGLDCIAGEIRLVHVGPQGTAPLI
                     ETPHWPGWSYNTIVRQGDVVDEIVKAEHEWRPDLMVLATQGHLDFLDALRGSTTERVL
                     RAAHCPVLAVPVRRIASL"
     gene            complement(2139..2522)
                     /locus_tag="VF089_21760"
     CDS             complement(2139..2522)
                     /locus_tag="VF089_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176643.1"
                     /translation="MESETVAGGDTAAVLDLGRLSGLRRHCEAGARATQEAVVLGIID
                     FGNEGSKNVVTAPTTVAVGEEFKRTITTFGGGCEREGTSGTIITATGATIMVYDITSA
                     VHPGVICTAEIKRLPHSSLPTAPAR"
     gene            complement(2583..3653)
                     /locus_tag="VF089_21765"
     CDS             complement(2583..3653)
                     /locus_tag="VF089_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX6176644.1"
                     /translation="MEPSKFFSLPAGAALMLLAGCSSHHAALERARADFTKTHQDSQI
                     SNHAPVAVHEAAQSLRRAEEVWENTGDQEEVKHLAYLAEQRTAIAREVAQRRIAESEA
                     QRLVEQRDKVLLEARRREAERAMQLAEARAREAEQARQQAEKSALAAQQARRGEQAQA
                     RQAELARQEAEKRAQEAETARQQAAKRAEELELARQQAEARAREAEQARQQAQADAAR
                     IQQLEKELSEFKARQTERGLELTLSGVLFEFDKAVLKPGALRGLSPLVAFLKNNPDHH
                     IALEGHTDSVGSESYNIDLSRQRAEAVRQFLVQNGVNTRNISTRGLGEAYPVASNNTE
                     AGRLQNRRVEIIISDQTAGSTG"
     gene            complement(3871..4236)
                     /locus_tag="VF089_21770"
     CDS             complement(3871..4236)
                     /locus_tag="VF089_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX6176645.1"
                     /translation="MKLTRTASYLTVGALVFSGCSSVTPPREELATAELAVREAQQSK
                     ASQHASLELRMATEKLNRAKQAMRDEDHVAARRLAEQALVDAQLAENKARSAEARAMA
                     RQLREGIETLRKEAERVSG"
     gene            4818..5621
                     /locus_tag="VF089_21775"
     CDS             4818..5621
                     /locus_tag="VF089_21775"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX6176646.1"
                     /translation="MTRSEKRRNRKNIASVIAVAGILSLGTAPAWAQQSKEGKATQSK
                     QSSQQQSKQSRQQDKIADNLIAAENLQDHRVVDANNREVGTITNLFIDPQSGRVQRAD
                     IDFNSSMFGADHKYSVAWDKLKVKQQGQDVVVSLDESIVKRVQQAGKSGRVAEGDGVY
                     SQRQDGGILGLGDSGRNQQPITASQLNAEQIRKVQQKLNQEGFHAGQVDGKWSSETQA
                     AIKNFQQSKGIKATGQLDERTLDQLGLEADEFRERNRSGSTSGSKPSNR"
     gene            5810..6208
                     /locus_tag="VF089_21780"
     CDS             5810..6208
                     /locus_tag="VF089_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEX6176647.1"
                     /translation="MGTIDTSAIEWLANGIEIFGVVTILAGAVVATTLFSLRVLQKTD
                     FKSAYTQYRSNLGRAILLGLEFLVAGDIIATILIEPTITRAGALGIIVLIRTFLSFSL
                     ETEIEGCWPWERARIRNDARSHNLDPDGFR"
BASE COUNT         1417 a         1664 c         1819 g         1311 t
ORIGIN      
        1 gtaatcgaag gaggtaaata aaatgaaaag gacgaaactt atcgtcgtca tggctgcggt
       61 cattttcgtg gccggcccga gcggatattt gctcgcccag cacatgcagg gagggccgat
      121 gatgcagtcg cccgcgcaac aaaagagtcc aactcaggag agcggcgaaa gttcccaaca
      181 gcgtgagtcg ccaacgggaa gcgagatcgt gccgccctat ggtatgggac ggggtggtgg
      241 gtacggacca ggatatggct acggcatggg acccggaatg atgggacccg gaatgatggg
      301 acccggaatg atggggccgg gaatgggaat ggcgggaggt ttcgggccgg gtatgggaat
      361 gatgggcccg atgatgcatt cgataatggg atcgccgcag atgatgggac ttatgatgtc
      421 catgcatggg gacatgatgg ctatgatggg tcgaatgata cagcactacg gaggcgagca
      481 gagtcccgaa gtgcagcaga agatgcagac agagatgttg caggaaatgg gtgacatcct
      541 cgtgaagcat ggaaaccgct tgaaagagag tgctaagtcg tcgggacgat gaccgctgag
      601 cggtgctgta catgaaacga acgcggcaca taggtgcgtg agcagctagc gagggcggcg
      661 cgcgaaaaac gggaaagaca ttgtctacgc tcagaaaaga tccgaccatc aaagatgggt
      721 gatcattgcg accgaaaggg cgaagcgtcc gcaccgcttc gtcgggttga cgaaaaccgc
      781 cggttttgag ttgggatcgg caatgtcgat caacacggcc ttccggagga gactgcccga
      841 atcatgcggg aggtgatgtc atgccttcca gaagtaatag tcggacggca ttgctggctg
      901 ctagtcaacc aacccagacc attaccggcc gttgcccggc gaccgagccc ggccacctcg
      961 ctcagatcct tggctggggc caggatctaa taggataatg cgacctcggc caagacgctg
     1021 ctgagtattt tctgtctcga tgactggagc gatttggcta ctttggctgc tgggataaaa
     1081 gctgagcgtc gccctgacga gtcggaatca ttgcgccggc gtggacgcaa gaagcatggt
     1141 gtcttaacgt gagcctaaca tctatagttg aaaagcagct acgtggaaat tttgtcatct
     1201 tccatccatc ggatttttcc gcggggagtg aagtggcctt tggccatgcg cttaaaatcg
     1261 cgatgcaggc aaaggcaaag ctcgacatca tgcacgttgt agagacacat attagcccgg
     1321 agaagccaga ctggcaggac tttccggccg tgcgagccac cttgagccgc tggggtattt
     1381 tgccggaggg tgttgggcgc gatgaggtcg ccaaggccgg ccttcatata aggaaaattc
     1441 tacaggcagg tgccgatccc ttgaaaacca tgctgcggca cttccgcaaa cttccgcctg
     1501 atctaattgt tctcgctacc catcagaggg aaggtcttga acggtggttg cataaagcgg
     1561 ttgcagagcc actcgcgcgg cgatcgggag caatgacgct ttttgtgccg agcgaagcga
     1621 aaggcttcat ttcagtcaag gacggttcgg ttaggctgaa aaaaattctg attccgatcg
     1681 atcacgatcc cgatccgcaa acggcgctca gcaaggcatc ccttctggca cagggccttg
     1741 attgtatcgc gggagaaatt cggctggttc acgtcgggcc gcagggaacg gcaccgctga
     1801 ttgagacgcc gcactggccc ggatggtcgt ataacaccat tgtgaggcaa ggcgacgtgg
     1861 tagacgaaat cgtaaaagct gaacatgagt ggcgtcctga cctcatggtg ctcgcaaccc
     1921 agggacactt ggactttcta gatgccttgc gcgggagcac aactgaaaga gtgctccggg
     1981 cggcgcattg tcccgtcctt gccgtcccgg ttcggagaat agccagtctt tagaaaacca
     2041 gggatccgat tcgttccatt tagtctttgc attcggcgca gaatccgttg gttgatctca
     2101 tcgacagtat gctgggaaac gtacagtcgt tgccggattt atcgagccgg cgctgttggc
     2161 aaagagctat gaggtaggcg tttaatttca gcggtgcaga tcaccccggg atggacggcc
     2221 gacgtgatgt catacaccat gatagtcgcg cctgtcgcgg tgataatcgt gccactggtg
     2281 ccctcgcgct cgcaaccgcc accgaacgtg gtgatcgtgc gcttgaactc ttcaccgacg
     2341 gcaacggtag tgggcgcggt tactacattc ttgcttccct cattaccgaa gtcgatgatg
     2401 ccgagtacga ctgcctcttg cgtggcgcgc gctccggctt cgcagtggcg gcgtaggccg
     2461 gataagcggc caagatcgag tacagcggca gtatcgccgc cagcaaccgt ttcactttcc
     2521 acctccagca ctccacggca gcatgggccg acaacggcag tagcgatatt ttccctgcta
     2581 acttacccgg tggacccggc agtctggtcg gaaatgatga tctcgacgcg ccggttctgc
     2641 aatctgcccg cctcggtatt gttggaggcg accgggtagg cctcgcccag gccgcgtgtg
     2701 gagatatttc ttgtattcac tccgttctgc accagaaact gccgcacagc ctcggcgcgt
     2761 tgccgcgaaa gatcgatgtt gtacgattcg gacccgacgc tgtcggtatg accctcgagc
     2821 gcaatgtgat ggtccggatt atttttaagg aatgcgacga gaggcgaaag gccgcgcaga
     2881 gcgcccggtt tcagaacagc tttgtcgaat tcgaagagaa caccgctcaa tgtcagttcc
     2941 aggcctcgtt ccgtctgcct cgccttgaat tccgacaact ctttttccag ctgctgaatc
     3001 cgagcggcat cggcttgagc ctgctgtcgg gcttgttcag cctcgcgggc ccgggcttcg
     3061 gcttgttgtc tggccagctc cagctcttcg gcgcgttttg ccgcctgctg gcgcgcggtc
     3121 tcagcttcct gagcgcgctt ctcggcctct tgccgcgcca actctgcttg gcgcgcctgc
     3181 gcctgctcgc ctcgccgcgc ctgctgtgcc gcgagagcgc tcttttcggc ctgttgtcgc
     3241 gcctgttccg cttcgcgagc gcgagcttcc gccaactgca tggcccgctc agcctcgcgc
     3301 cttcgcgctt ctaaaagtac tttgtcgcgt tgttccacaa gccgttgcgc ttcgctctca
     3361 gctattcgtc gttgcgctac ttcgcgcgca atagcggtcc gctgttccgc taaatacgcc
     3421 aagtgtttta cttcttcctg atctccggtg ttttcccaaa cttcctcggc gcgccgcaga
     3481 gattgggccg cttcatgcac cgctaccggc gcatggttgg agatctgtga atcctgatgg
     3541 gtcttggtaa aatccgcccg cgcccgctcc aaggccgcgt gatggctgct acagcctgca
     3601 agcagcatga gcgctgctcc ggcgggtagc gaaaaaaact tcgatggttc catgactctg
     3661 gtctccttct attgaaaggt tgtaatttac ccgcacacct cataacctct gagagatcgt
     3721 aatttgccgc gcactgataa aatcgcgggg ctcgtaaatt cgaatccggc cagcaaagat
     3781 caaagtattg gagcggcgag cccgctggag cgaaatgcag cagcgggcgg caactacgat
     3841 gctatcccga ctgcggattt ctaaattgat tcatccgctc acccgctcgg cctctttacg
     3901 cagcgtctcg ataccttcgc gcagttggcg cgccattgct ctggcttctg ccgaccgcgc
     3961 cttattttcg gccagctgtg catcgactag cgcctgttcg gccaaacgcc gagcggcgac
     4021 gtggtcctca tctctcatcg cttgcttggc ccggttcagc ttttctgtcg ccatgcgcag
     4081 ttccaagctg gcgtgctgcg aagccttgga ttgttgcgcc tcgcgcactg ccagttcagc
     4141 ggttgccagc tcttcgcgcg gcggcgttac gctgctacat ccggaaaaaa cgagtgcgcc
     4201 aacagtaagg taagatgccg ttctcgtaag cttcatattg ctcccttcga tacttttcac
     4261 ttcatgcgcg ctccagaagc aaacccgcac ctgaacacgc cccgcaccgt ttctgatacc
     4321 gcaagggcga tgccacctaa aaggacaatt cgaagttagt agctgtcgcc ggagatgtca
     4381 gatgtcgaga gaagtgtgca gaaaaaacgc ttgcagtgtg ctccatgtaa acaaatcgag
     4441 tttcagccaa cccacgatga cagcgcctca tactgtgctc ctcaagaagt gaacgtcgcg
     4501 caaacggagt tttgtcttgc tgatgcgcag gtgagacgac tgcggggaag ataacctaat
     4561 tggtgtcgca acaagcggac aaccgagctt cgccgatttc ctctgcggca gatttcctag
     4621 ctcttgatcg cgcgaagatt cagctacaca ggcataacgc ttgcgttact agcgccgaaa
     4681 cggaggacgc aatcatggaa aaaaaaatcg ttaaaaacac gagggatccg cctgagggag
     4741 agaccaagca tcgctaaact gaatatcgaa aactgaatct aaaaacacga acagcaaaaa
     4801 actagcggag ggaaatcatg acacgaagcg aaaagagacg taacaggaaa aacatagcgt
     4861 cggttattgc tgtggcaggt atactgtcac tgggaacagc cccagcatgg gcgcagcaga
     4921 gtaaggaagg taaggcgact cagagcaagc agagctccca gcagcaaagc aagcagtcac
     4981 ggcaacagga caagatcgct gataatctaa ttgcagccga aaatttgcag gaccatcggg
     5041 ttgtggacgc aaataaccgg gaggttggaa caatcaccaa tctgttcatc gatcctcaga
     5101 gcggcagggt tcaaagagcg gatattgact tcaacagctc gatgtttggc gccgatcata
     5161 aatattccgt tgcgtgggac aagctgaagg ttaaacagca gggccaagat gtggtggtga
     5221 gcttggatga gtcgatcgtc aagcgcgtac aacaagcggg aaagagcggc agagtggcgg
     5281 aaggggatgg agtctacagc cagcgccagg acggtggcat tctcggcttg ggcgactccg
     5341 ggaggaatca acagcccatt acagcatcgc agctaaatgc ggagcaaatc cgcaaggttc
     5401 aacagaaact caatcaagaa ggctttcacg ccgggcaagt cgacggcaag tggagctccg
     5461 aaacacaggc agccatcaag aattttcaac agtccaaggg gatcaaagcc acgggccagc
     5521 tagacgagcg gacactggat caattgggtc tggaggcgga tgaattccgg gagagaaacc
     5581 gctccgggag cacgagtggt tcgaagccaa gcaaccgcta aacggaaatc gcaagtcgaa
     5641 taggacggag aaagcatggg cggaaaaagg aggggacact cccctccttt ttttttgcca
     5701 accctggagt ggagaccggc gagaagtgcc gtgcccgccg caggatggcg taagcaaatc
     5761 tcgcacgtcc gcatgctgct gctctggcgg cgcctcgaag agcaagaata tgggaacgat
     5821 cgacacttct gcaatcgaat ggttggccaa cggtatcgaa attttcggag tagtaacgat
     5881 ccttgccgga gccgtggtgg cgacgacgct gttttctcta cgggtcttgc aaaaaaccga
     5941 ttttaagtcg gcttacactc aataccgttc aaatctcggc cgcgcaattc tactcgggct
     6001 cgagtttctc gttgccggcg acattattgc cacgattctc atcgagccga cgatcaccag
     6061 ggcgggagct ctagggatta tcgtcctgat tcggacattt ctgagttttt cgttagagac
     6121 ggagatcgag ggttgctggc cctgggagcg ggctcgtatt cgcaatgacg ctcggtccca
     6181 taacctcgac cctgacggat tccgttagcc g
//
LOCUS       DASUTG010000933         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1626482, whole genome shotgun sequence.
ACCESSION   DASUTG010000933 DASUTG010000000
VERSION     DASUTG010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626482"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..752)
                     /gene="purN"
                     /locus_tag="VF089_21785"
     CDS             complement(96..752)
                     /gene="purN"
                     /locus_tag="VF089_21785"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX6176648.1"
                     /translation="MARQLPIGVLISGNGTNLQAIIDAIEAHALDAKIEVVLSNKADA
                     YGLARAKKHNLYTAVLDHTSFSSREAYDEAVLDILRRCGVELVVLAGFMRLLSPLFIK
                     AYSNRIMNIHPALLPAFPGLRVQKKALEHGVRFSGCTVHFVNDDCDEGPIIMQAVVPV
                     FPDDSESVLAARILKQEHRIYPRAIQLYAQGRLHIVGRRVMVDDLPKDEKQVLIQPQI
                     "
     gene            complement(764..1786)
                     /gene="purM"
                     /locus_tag="VF089_21790"
     CDS             complement(764..1786)
                     /gene="purM"
                     /locus_tag="VF089_21790"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX6176649.1"
                     /translation="MTYAAAGVSIEAGDELVRRIARVAKKTKVPGLLAGVGGFSALFD
                     LKARRYRHPVLVSSTDGVGTKLKIAFATGIHETVGIDLVAMSVNDILTQGAEPLFFLD
                     YFVCGKLDVNIAEAVVRGIAQGCHQSGCVLIGGETAEHPGDFPDGEYDLAGFVVGALE
                     KKKILNPEAIIPGDVLIGLPSSGLHSNGYSLARKVLLSRGRLKLGQRLPELGRTLGEE
                     LLVPTRIYAKTLRTLLARWPIKGAAHITGGGIVGNLPRVLPPGARALIRRDSWPIPPI
                     FGMIAKLGAVSRDEMDRTFNNGLGMILVIGKKAADGAMRSLKQSGEKPVVIGEIRKGP
                     RGADMI"
     gene            complement(1880..2674)
                     /locus_tag="VF089_21795"
     CDS             complement(1880..2674)
                     /locus_tag="VF089_21795"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6176650.1"
                     /translation="MPSLIAADNVCRVFTSGARMVNALDRISFEIQTGEFVSIVGPSG
                     CGKSTLLKILSGLLPASSGTIVMSGKAVDEPVENVGMVFQAPVLLKWRPVLGNILLPV
                     EFAKRNVAGHMERARSLIKLVGLEGFEEMYPHQLSGGMQQRVSLCRALVTDPQLLLMD
                     EPFGALDAMTRDELDLELLRLWEEKKKTVLFVTHSIQEAVFLSDRVFVMSARPGRLLE
                     VLSIDLPRPRTLEMMSSVGFGEYTLRIRSLLASAMGTPEAISGSTA"
BASE COUNT          643 a          798 c          724 g          578 t
ORIGIN      
        1 aaattgttcg ccgtcaatgc gcgttaccaa atagctcagc agcccgacac tgaccaaaag
       61 cttgaggaca aagatgagga tgctcttgcg gtttttcata tctgcggttg gatgagaacc
      121 tgcttctcgt ccttcggcag atcatccacc atcactctac ggccgacgat gtgaagtctt
      181 ccttgggcat aaagctgaat cgctcgcgga tagatgcgat gttcctgttt caggatgcgg
      241 gctgcgagaa cgctctcgct atcgtccgga aacaccggca ccaccgcctg cataatgatc
      301 ggaccctcgt cgcaatcatc attgacaaag tgaaccgtgc aaccggagaa tcgcacacca
      361 tgttcgagcg cttttttctg cacgcgcaga ccggggaagg ccggcagtaa cgccggatgt
      421 atattcatga tgcgattcga ataagctttg atgaacagcg gcgacaatag ccgcatgaaa
      481 ccggctagta cgaccagctc gacaccgcag cgccggagaa tatccagtac cgcctcatca
      541 taggcttcac ggctggaaaa ggacgtgtgg tccaatactg cggtataaag attatgcttt
      601 ttagctcgtg ccaggccata ggcatccgct ttgttgctca agacgacctc gatcttggca
      661 tcgagcgcgt gcgcttcgat ggcatcgatg atggcttgga gattggtgcc gttgcccgag
      721 ataagaacgc ctatcggaag ctgtcgtgcc ataacgtcgt gctttagatc atgtctgcgc
      781 cgcgcggacc tttgcgaatc tcgccgatca caaccggctt ctctccgctt tgcttaaggg
      841 aacgcatagc gccgtcggca gcttttttgc caattactag gatcataccc aaaccgttat
      901 taaaggtccg gtccatctcg tctcgagata ccgcgcctaa cttggctatc atgccaaata
      961 tcggcggaat cggccagctg tcccggcgga taagcgcgcg cgcgcccggc ggcaggacgc
     1021 gtggcagatt accgacgatt ccgccaccgg tgatgtgcgc cgcgcccttg ataggccaac
     1081 gggccaacag agtgcgcagg gtcttggcgt aaatgcgtgt cggcactaga agctcctcgc
     1141 ccagagttcg gccgagctcg ggcagccgtt gcccgagttt tagacggccg cgcgacagga
     1201 gaaccttacg ggctaatgaa tagccgttgc tgtgaagccc gcttgaagga agtccgataa
     1261 gaacatcgcc tgggataatc gcctcaggat tgagaatttt ctttttttct agcgcgccga
     1321 ctacgaatcc ggcaagatca tactcgccgt ccgggaaatc gccaggatgc tcggcggtct
     1381 cgccaccgat caagacgcag cccgattggt ggcaaccctg tgcgatgcca cgaaccacag
     1441 cctcggcgat attgacgtcg agtttgccgc aaacgaaata atccaagaag aacagcggct
     1501 cagcaccttg tgtcaaaata tcgttgacac tcatcgccac cagatcgatg ccaactgtct
     1561 catgaatacc ggtcgcgaaa gcaatcttga gcttagtacc gaccccatcc gttgaggaca
     1621 ccagaacagg gtgacggtag cggcgcgctt ttaggtcgaa gagcgcacta aaaccgccaa
     1681 cgccggccag caaccccggc actttagttt tcttggcaac gcgggcgatc cggcgcacca
     1741 gctcatcgcc tgcctcaatg ctgacacccg ccgcggcgta ggtcattgct cgttgtctgg
     1801 ccaaaaattt agcgtccgtt cctgtgttgc ttgaggtaag cgtgattcgc cgaatgaggg
     1861 cgcagcgccg gaagcaaatt catgcggtgg aaccggaaat cgcctccggc gtacccatcg
     1921 cagaggcgag caagcttctg attctgagcg tgtactcacc aaagcccacg ctgctcatca
     1981 tctccagagt tcgcggtcgg ggaagatcaa tcgacaggac ttccagcagc ctgccgggcc
     2041 tcgcagacat aacgaatacg cgatccgata aaaacaccgc ttcctggatg ctgtgtgtaa
     2101 caaacaaaac cgttttcttc ttttcttccc ataacctcag tagctcgaga tccaattcgt
     2161 cgcgggtcat agcatcgagc gccccaaatg gctcgtccat gagcagcagt tgcggatcag
     2221 ttaccagggc tcggcaaagt gagactcgct gctgcatgcc gccggagagc tgatgcggat
     2281 acatctcttc gaagccctcc agcccgacca acttgatcaa cgagcgagcc ctctccatat
     2341 gaccggcaac attgcgcttg gcgaactcca ctggcaatag aatatttccc aataccggac
     2401 gccactttaa cagcaccggg gcctgaaaca ccatgccgac gttttccacc ggttcgtcga
     2461 cggctttgcc gctcatcact atcgtaccgg atgacgcggg gaggagcccc gaaagaattt
     2521 tcagtagcgt gctcttgccg cagccgctgg gtccgacaat ggagacaaac tcgccggttt
     2581 gaatttcgaa agaaatgcga tccaaagcat tgaccatcct ggcgccgctg gtaaacaccc
     2641 ggcagacatt gtcagcagca attagtgacg gcattcggac ccagagtaag gaaggcaatt
     2701 atctcttgat aaactgatcg gtacaaacct cctgcaaccg cta
//
LOCUS       DASUTG010000934         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1181059, whole genome shotgun sequence.
ACCESSION   DASUTG010000934 DASUTG010000000
VERSION     DASUTG010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181059"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2665
                     /locus_tag="VF089_21800"
     CDS             <1..2665
                     /locus_tag="VF089_21800"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX6176651.1"
                     /translation="LQAGRWVAPVVRRNVVSFARRFIAEEKTDELIPILSGLRAEPAA
                     FTLDVVGEATVSDSEAVAMQNRYLTLLRSLSGAAAAWPSQPQIDESPFGPIPRVNLSV
                     KLSSLCARFDPLDPETESVVRQRLRSILREAGRLGAALTIDMEQYAFKPLTFQIFRAL
                     LEEEEFAATPQVGLAVQAYLRDTGSDLADLIGWAKQRGRRIGIRLVKGAYWDSEVAWT
                     QQKNWPVPVFLEKAETDASYERLSRLLLESHDVVDAAFGSHNLRSLAHAIVTANHLGV
                     PRNGYEIQMLYGMAEPFRRAIIENGQRVRVYLPVGELLPGMSYLIRRLMENTSNTSFL
                     RQTYADKKDVESLIAAPTAAARTSPTHPQLAYADETSPFRNEPAIDFAHSEKRAQFYK
                     ALEATSERIKRQAQTVRGGEWLESINPANLKEVVGRVKIASTEEADEAIHTATRFFPK
                     WRATPAKKRADILLRAAEIMRRKRWELASWEVFEVGKGWREADGDVIEAIDYLEYYAR
                     EMLRLARPRITQQLAGESNVYLFEPRGIAAIIAPWNFPLAILTGMTAAALVTGNCVIM
                     KPAEQSPMMAAHLLDILRQAGLPLPACQLLQGGGVLGAHLVPHQSIHAIAFTGSREVG
                     LEILKQAYTTRAGQSHVKRVVCEMGGKNAVIVDSDADLDEAVVHIIDSAFGYQGQKCS
                     AASRIILLNEVHDRLLSRIIEAVKSLRIGPPSDPRNFIGPVIDGEAKERIENYIDIGT
                     KEAQSALRMAAPDGGFFVGPAIFSEVEPQSRLAQEEIFGPVLSTIRARDFDHALEIAN
                     SSSFALTGGVFSRSPAHIELARNEFRVGNLYINRGITGAVVERQPFGGLKLSGIGSKA
                     GGPDYLLQFLEPRTISENTLRHGFVPTPNGL"
     gene            2758..>3434
                     /locus_tag="VF089_21805"
     CDS             2758..>3434
                     /locus_tag="VF089_21805"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6176652.1"
                     /translation="MKAELNDKTTGQVAKSDYRAPWWLPGGHLQTLYAYFLKRETHLC
                     LWRERWETPDNDFIDLDWLDSSDGSANLLVLFHGLEGCSSSHYAQSLMNYAGRRSWRG
                     VISHFRGCSGEHNRLVRAYHSGDSSEIDWILRRLKENNPKAEIYAVGVSLGGNVLLKW
                     LGEQGEHALEVVEKAAAVSAPVDLAAAAGVLDRGCRKLIYTRYFLRTLKQKSLYKIAK
                     HQLKIDLR"
BASE COUNT          729 a          961 c         1006 g          738 t
ORIGIN      
        1 gcttcaggcg ggaagatggg ttgcgccggt agtgcgccgc aatgttgtga gttttgcgcg
       61 ccgcttcatc gccgaggaaa agaccgacga actgattccg attctctccg ggctcagagc
      121 cgagcccgca gcctttacac ttgacgtggt tggcgaggcc acggtcagcg acagcgaagc
      181 cgtggcgatg caaaaccgct atctgacttt gctccgcagt ctatctgggg cggcggcggc
      241 atggccctca cagccgcaaa tcgacgagtc gccgtttgga ccaatcccac gggtcaatct
      301 atcagtcaaa ctttcttcac tgtgcgcgcg cttcgatccg ctcgaccctg agactgaatc
      361 cgtcgtgcgc cagcggctac gttccatact gcgcgaagca gggcggctcg gcgccgccct
      421 gaccatcgat atggaacagt acgcgttcaa gccgcttacg tttcaaatat tccgagcgct
      481 ccttgaagaa gaagaatttg cagcgacgcc ccaagtgggc ctggcggttc aagcttatct
      541 gcgcgatacc gggagcgatt tggccgatct gattggctgg gccaaacagc gcggccggcg
      601 catcggcatt cgactggtta aaggagccta ctgggacagc gaggtagcat ggacgcagca
      661 gaaaaattgg cccgttccgg tatttttaga gaaggcggaa actgatgcca gttacgaacg
      721 gcttagccgc ctgttgctgg aaagccatga cgtcgtcgat gccgccttcg gcagccacaa
      781 cctgcgtagt ctcgcccatg cgattgtcac agccaatcat ctgggcgtgc cgcgcaatgg
      841 ctatgaaatt caaatgctat acggtatggc cgaaccgttt cgccgggcga tcattgaaaa
      901 tggccagagg gttcgtgtct atcttccggt tggcgagctg cttccgggaa tgtcctatct
      961 gatccgccgc ttgatggaga acacttcgaa tacttctttc ctccggcaga cctatgccga
     1021 caagaaggac gttgagagtc tgatcgccgc gccgactgcg gcggctcgaa cctctccgac
     1081 tcatccgcaa ctggcttatg cggacgaaac ttcgccgttt cgcaatgagc cggcaatcga
     1141 tttcgcgcat tcggagaaac gcgctcaatt ctataaggcg ctggaggcca ccagcgagcg
     1201 gatcaaacga caggcccaga cggtacgagg tggtgagtgg ttagaatcga tcaatccggc
     1261 caatcttaaa gaagtcgttg gtcgagtaaa gatagccagc acagaagagg ccgacgaggc
     1321 tatccatacg gcaactcgct tcttccccaa gtggcgcgcc acgcctgcga aaaaacgcgc
     1381 ggacattctc ctgcgtgccg cggaaatcat gcgccgcaaa cgttgggagc ttgcatcgtg
     1441 ggaagtgttt gaagtcggca agggctggcg tgaagcagac ggcgatgtca tcgaagccat
     1501 cgattatctc gaatactatg cccgcgagat gctccggttg gcccggcccc ggatcaccca
     1561 acagctcgcc ggcgagagca atgtttatct gttcgaacca agaggtatcg cagccatcat
     1621 tgcgccttgg aattttccct tggcgattct caccggcatg acggcagctg cgctggtgac
     1681 cgggaattgt gtgatcatga agccggcgga gcaatcaccg atgatggccg ctcatctgct
     1741 cgacattctg cgccaagctg gattgccact gccggcgtgt caattgcttc agggcggcgg
     1801 cgttctgggc gcgcatctcg tgccccatca gagtattcac gcgattgctt ttaccggctc
     1861 gcgcgaagtg gggttggaaa tattgaagca ggcctatacg actcgagccg gtcaaagcca
     1921 tgtcaagcga gtggtatgcg agatgggcgg caagaacgcc gtgatcgtcg acagcgatgc
     1981 cgatctcgac gaggccgttg ttcacatcat cgattccgcg ttcggctatc agggccagaa
     2041 gtgctcggcc gcttcgcgca tcattttgct caacgaggtg catgatcgct tgttgtcgcg
     2101 catcatcgaa gccgtcaaaa gcctcaggat cggccccccg tcagatccgc gcaattttat
     2161 cgggccggtg atcgacggtg aggccaagga acgaatcgag aattacattg atatcgggac
     2221 caaagaggcg cagtccgcgc tgcggatggc tgcgccggat ggtggctttt ttgtcggccc
     2281 ggcaattttt agcgaggtcg agccgcagag ccggttagct caggaggaga tttttggtcc
     2341 ggtgctctcg accatcagag cccgcgactt cgatcatgcg ctcgagatcg ccaatagttc
     2401 ctctttcgcc ctcaccggtg gggtattttc ccggtccccc gcgcatatcg agctggcgcg
     2461 caacgaattc cgagtgggca atctttatat caatcgcggc atcaccggcg ccgtagtgga
     2521 gcgccaaccg ttcggcgggc tcaagctgtc cggtatcgga tcaaaagccg gcgggccgga
     2581 ttatctgctg cagtttctcg agcccaggac catttctgag aataccttac gccacggttt
     2641 tgtgcctacg ccaaacggac tctaaatcgt tcaagccgtt caagtcgctc caatggttca
     2701 agtcgtcaga cctgccgaca attggtgcga atccggccag actcagtcgc ccgaccgatg
     2761 aaagccgagc ttaacgacaa aacaacgggg caagtcgcca agtcagatta ccgggcgccg
     2821 tggtggttgc cgggtggtca cttacagacc ctctatgctt actttctcaa gcgcgagacg
     2881 catctttgct tgtggcgcga acgttgggaa actcccgata acgattttat cgatctcgat
     2941 tggctcgatt ccagcgacgg cagcgcgaat ctgctagtgc tttttcatgg gctggaaggc
     3001 tgttccagca gtcactacgc tcaaagcttg atgaactatg cagggcgccg gagttggcgc
     3061 ggcgtgatat cgcattttcg cggctgcagt ggcgagcaca accgtctggt tcgcgcctat
     3121 cattcaggcg attcgagcga gatcgactgg attcttcggc gtctcaaaga gaacaatccg
     3181 aaagcggaaa tatatgcagt cggtgtgtcc cttggtggca atgtgctgct caaatggctc
     3241 ggcgagcaag gtgagcacgc cctggaagtt gtcgagaagg cggcggcggt ttcagctccg
     3301 gtagacctag cagccgctgc cggtgtcctg gatcggggtt gccgtaaact gatctacacg
     3361 cgctattttc tcagaacgct caagcaaaag tcactgtata agattgccaa gcaccagtta
     3421 aaaatcgatc tgcg
//
LOCUS       DASUTG010000935         7991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_739097, whole genome shotgun sequence.
ACCESSION   DASUTG010000935 DASUTG010000000
VERSION     DASUTG010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739097"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1430
                     /locus_tag="VF089_21810"
     CDS             447..1430
                     /locus_tag="VF089_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6176653.1"
                     /translation="MLEGVNGKEDWILEESVFVGIEVSKATLDIVMRPGGSRESIPNQ
                     EAQIGELVRRLSTLRVTLLVVEATGGVERRIVRALAAAELPVIVVNPRQVRDFAKATG
                     RLAKTDKIDAEILAHFGEAVRPALRAVPEPASEELRALITRRRQVTEMLTAETNRLAH
                     SAKAVRKRIEAHIRWLEAELDRVDSELDQSIQQSPIWREQEELLKSVPGIGPVISRTL
                     LAELPELGKLNRKQIAALVGVAPLNWDSGTLRGRRAIWGGRSAVRAALYMAALVASRR
                     NSTIQDFYRRLRNAGKAPKVALVACMRKLLTILNAMIKHKTHWSVNTVQAT"
     gene            1623..1991
                     /locus_tag="VF089_21815"
     CDS             1623..1991
                     /locus_tag="VF089_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX6176654.1"
                     /translation="MSIRSNTMKKLSGIAAVAVVSGTLLAGCAGGGLTTREKGAGIGA
                     LGGAAAGGLIGSAVRHPGAGAAIGAALGLGAGAVIGDQLQGRENQAYEHDQQIYRNQQ
                     EINRQREQLEQMRRRDNEYY"
     gene            2220..3104
                     /gene="rfbD"
                     /locus_tag="VF089_21820"
     CDS             2220..3104
                     /gene="rfbD"
                     /locus_tag="VF089_21820"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX6176655.1"
                     /translation="MALRHRSTFDFVKVLVTGAAGLIGSHLAQRFTSNHDVLALKRKD
                     LDITDPAAVRSRVAAERPSLLVNCAVVQVDEAEQDPAKAAAVNVQGARFLAEAAAQSG
                     AEIIQFSTQYAFDGEIIGRAPYTIKDQPRPVNIYGKTKVAGEEAVRAACAQSYIIRTS
                     WVYGRGKNSFLCTVHDDLGSGRRVRAIDDVWSSTTYVGDLIDRCSEILKLRHYGTYQI
                     VNEGVCSYYDFAIEAGRLAGLEKGQLDSLIEVVHERDMQRIAPRPCYTPMRCLLSEEV
                     GLAPMRHWRDALAQYVRR"
     gene            3375..4349
                     /locus_tag="VF089_21825"
     CDS             3375..4349
                     /locus_tag="VF089_21825"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176656.1"
                     /translation="MKTLFFCISVLFLVTFSRTATAQKLTLAHVAIHPGQGMFWVAKD
                     SGLLAKHGFSAEVVLIPGSPRTVQALIAGDLDYAVAGAPAYVRARTQGADVVILSSLS
                     GYSSQRVLLKPDSTVQTFNDIRGKTIGVTQYGSAGDSFIRGALKKSGIKESEVTILQM
                     AGTPGVSQALEAGRIEVGVLGESGALLVFRGKARFLKGASSREFGFHALDAPLGTTER
                     KIKADRGSVLRFMQAYVEALHYFKTNRAGTIKILQKYMAGLSEEHVALWYDEYREGLV
                     SLPYPDDNGLRAELDLINVPRSQSLAPYVNKSFLDEIKKSGLVERLYK"
     gene            4364..4729
                     /locus_tag="VF089_21830"
     CDS             4364..4729
                     /locus_tag="VF089_21830"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6176657.1"
                     /translation="MAKIRHLAIKAEDQERAATFYKETFGMTEAWRGPVREDGHRAIY
                     LTDGYINMAILPARGGREGIDHFGFQVEDLESTLKTAASTGAKSDSYKKPRDGRFAEV
                     GVQDPVGTPVDVSVHGWKA"
     gene            4796..5797
                     /locus_tag="VF089_21835"
     CDS             4796..5797
                     /locus_tag="VF089_21835"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176658.1"
                     /translation="MKKTSWLIIVLFYNLVMHASASFAAEPSKLLIAHGAISNNVAPL
                     WIAKEQGIFRKYGIEADLVFIIAGRAMQAMLAGQVPVGLVGGTHVTNANTGGGDFTMI
                     LGLENKLDYIFVAKPTIKGAEDLKGKKVAIGTPAGSASLATYVSLDHLGLVPRRDQIT
                     LLGIGGVPERMSALRSGSVEATSLSPEIGQVVVSEGYRVLIDLGKEGVPFQSSGLVVP
                     RSLIKTNPQLVENIAKATVEGAAFVIKPANKEIVLRSLGRNLRLDKPDRVEKAYQGLL
                     ANLPRPPCPSMEGVSAMLKLMAQHGLNAKASQVKPEEIADMSYCKKFEESGFFRSVY"
     gene            complement(6072..7322)
                     /locus_tag="VF089_21840"
     CDS             complement(6072..7322)
                     /locus_tag="VF089_21840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176659.1"
                     /translation="MADSPEIELPPPSSNIRPWSSLLIRDYRLVWLASVFNAVAVQVR
                     NVAGLYQVYEISSSPFQLGLTGFFQAFPFVIFGLFAGAVADTFDRKKLLIATVSIQFI
                     PSLLLGLLTVTGGVQVWHVYTLGFLGALVEVFNWPARSALIPRLVPRPLLMNAVTLNT
                     IIIQMSFLLGPAIGGVLIDQLGLVTTYFIAAAMLMPAVLAILAMRTSGVPEGVRRRVN
                     LRSIFEGIEFIWVQRIILSLFLLDFGVTLVGFYRPILPIFAADVFHTGASGLGLLYGA
                     PSLGAIVGSVALLMLGDIRRKGVLVVAAALIFAGSLALLGIAHWFWMAVGATLILGFT
                     DSVSVAIRRTVVQLQAPDGMLGRASSLISVFAQATNGLGAILAGAAAHYVGAPNALLL
                     GSLLCFIMISAICIAIPQLWRYKS"
     gene            complement(7566..7859)
                     /locus_tag="VF089_21845"
     CDS             complement(7566..7859)
                     /locus_tag="VF089_21845"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEX6176660.1"
                     /translation="MCTMIVHQAKIEGRGKNGGDWFELREANISYDHPYDMPLEHALN
                     IDFVNEAKGPAARVAVELTIEGARNLVKSIEAVLAQAEERGVLEDIRVAASSR"
BASE COUNT         1869 a         2123 c         2315 g         1684 t
ORIGIN      
        1 ccgtcgagct gccggcgatg gacaaggtga tcggtccgta cgatgagatc aaagtattat
       61 cctatccggg cgtgcccgcg aatccagcgc gcggcgctct gtcgctggaa ggaagggaag
      121 tgattatcgg cggagcggat atttggggcg agcaggcatg ggtatgcaat gattactagc
      181 aagctgctga aaaagactca taggcttcgt tgccgctcaa tcgcctcgct acgacgtact
      241 ataagtacgc ctccactcgt caattttcgc gcgccttcta tcttcatttc gttgtatgaa
      301 catactcgaa acaaatagga cgggacagat cgatcgcgcc tgggtcgtga gaggccgtag
      361 gcaagccagg gtcgtcctgt cctacctctg cgagagctcg aacagttgcc tgggctgata
      421 gcccggatca tgtggaaggt tgagccatgc tggagggggt taacgggaag gaggattgga
      481 ttttggaaga gagcgtgttt gtcggaatcg aggtatccaa agcgacactc gatatcgtta
      541 tgcgtccggg cggtagtaga gaatctatcc cgaatcaaga ggctcagatc ggggagctgg
      601 taagacggtt gagtactctg cgagtgactt tgcttgtggt ggaagccacc ggtggggtag
      661 aacgtcggat cgtgcgtgca ctggcggctg ccgaactgcc ggtgattgtc gttaatcccc
      721 gccaagtgcg tgacttcgcc aaagctaccg gccggttggc caagaccgac aagatcgatg
      781 ccgagatcct ggctcacttt ggagaagcgg tgcggccggc cttgcgcgct gtgccggagc
      841 cggccagcga agagctgcgc gcgctcatca cccggcgacg ccaagttaca gagatgctca
      901 cggccgaaac caatcggctt gctcatagcg ctaaagcggt acgcaaacga attgaggcgc
      961 atatcaggtg gctggaggcg gagcttgatc gagttgatag cgagcttgat cagagcatcc
     1021 agcagagtcc gatctggcgg gagcaggaag aattgctcaa gagtgtgcct ggaatcggac
     1081 ccgtcatcag ccgcacgctg ttggctgagc tgccggaact gggaaagctc aaccgaaaac
     1141 agatcgctgc actggtgggt gtggcgccgc ttaattggga tagtgggaca cttcgaggcc
     1201 gccgggccat ttggggcgga cggtcggcgg ttcgtgccgc gctgtacatg gctgcgttgg
     1261 tcgccagcag acgtaactcg actatccaag atttctacag acgattgcgt aacgccggca
     1321 aagcgccgaa ggtagctctg gttgcctgca tgcgcaaact gctcacgatt cttaacgcga
     1381 tgatcaaaca taaaactcat tggtctgtaa acactgttca ggccacttga cttttaagac
     1441 agttgcttgc ctctggatct ttttgagcag cctgcaagag gctttttcca gcaccctact
     1501 agacgctaac agttgtgcca aaagccacca ttaacaaggg ttaggatgtt tcaaggtgca
     1561 gccgatcatg taaacgaagt gctacttcat tcgtctaaac ggcaaaagga gagactgggg
     1621 ccatgagcat acgatcaaat acaatgaaaa aactgagcgg catcgcggct gtagcggtcg
     1681 tgagcggaac tttgttagcg ggttgcgcgg gcggggggct cactacccgt gaaaaaggcg
     1741 ccggcattgg cgctctgggc ggcgcagccg ccggcggcct catcggttcc gcggtgcgtc
     1801 atcccggcgc gggcgcagcg atcggagcgg cacttggtct gggcgcggga gcggtgatcg
     1861 gcgatcagct gcagggccgc gaaaatcagg cctacgaaca cgatcagcag atctatcgca
     1921 atcagcagga aatcaaccgt cagcgcgagc agctcgaaca aatgcgccgc cgcgataacg
     1981 aatactacta ggctcgaaca tcagtggagc ggcactgggc tgatgtggaa caccttcttg
     2041 caacaatggg actatcttct actgaacgtg atagtaggtc tgtacatgat gctcggtgcc
     2101 gctttgggtt tgctcttgat ctggcttttt ctcgcagctc ccactccgta aaaaactgct
     2161 agtctcgacc cgcgcactgt tgcgcgcttt cctctagagc ttcggttccc gcactcttga
     2221 tggccctgcg ccatcgttct acattcgatt tcgtgaaggt actcgtcacc ggcgcagccg
     2281 gcctaatcgg cagccatctc gcgcagcgat ttacaagcaa tcatgatgtg ctcgcgttga
     2341 aacgaaagga tctcgatatc accgatcccg cggcggtccg aagtcgtgtt gccgcagagc
     2401 gaccatctct gctggttaac tgtgcggtgg ttcaggtcga cgaagccgag caggaccccg
     2461 ccaaagctgc ggctgtaaac gtgcaaggag ccagatttct cgctgaggcg gcggctcagt
     2521 ctggcgcgga gatcattcaa ttcagcacgc aatacgcctt tgacggcgaa atcatcggac
     2581 gcgcgccgta caccatcaaa gatcaacctc gaccggtaaa tatctacggg aaaaccaagg
     2641 ttgccggtga agaagctgtg cgcgctgctt gcgcgcaaag ttacatcatc cgcacttcat
     2701 gggtttacgg tagaggcaag aatagctttc tctgtaccgt gcacgacgac ctcggctccg
     2761 gcaggcgcgt tcgcgccatt gacgatgtct ggtcgagcac gacttatgtt ggagatctca
     2821 tcgatcgttg ctcagagatt ctcaagcttc gccattacgg cacctaccag atcgttaacg
     2881 agggcgtttg ttcatactac gatttcgcta tcgaagcggg acgcctcgcg ggactcgaga
     2941 aagggcagct cgactcgctt atagaagttg tccacgagcg cgacatgcaa aggatcgcac
     3001 cgcggccttg ctacacgcct atgcgctgcc tgctctcgga agaggttggc ttggcaccga
     3061 tgcgccattg gcgcgatgcg ttggcgcaat atgtgcggag gtagcacatt aaatagccgt
     3121 gaatcgtctg cctgcgtgcg ctatcttcac cctaccaacc agctgcctag cagcttaaga
     3181 gatcgccttt actccagggt gccggttcgc attcgcaaaa tcagttggaa cctgtgagca
     3241 gccagtgccg atcaggacac ccggtataca aaaccgaaat tgacagtatc ggtgaaaact
     3301 aaatgaaggg gatgtgacgc gctgcaggcg ttgacatgac ctacagacca cagtaaaccg
     3361 ggaaccgaac tacgatgaaa accttatttt tttgtattag cgttcttttt ctcgtcacgt
     3421 tttctcgaac ggccacggca cagaaactga ccttggcgca cgtggcgatc catcctgggc
     3481 aaggtatgtt ctgggtcgct aaagactcgg gtttgcttgc caagcacggc tttagcgccg
     3541 aagtcgtgtt gattcccggc agtcctcgaa ccgtccaggc gctgattgcc ggagatctcg
     3601 attatgccgt tgccggcgcg cccgcatatg tgcgcgctcg gacccagggc gccgatgtcg
     3661 tgatcctttc atctctatcg ggatattctt cgcaaagggt tctgctcaaa cccgattcaa
     3721 ccgtgcagac gtttaacgac atacgaggga agaccatcgg cgtcacgcag tatggctcag
     3781 cgggggattc ctttatccgc ggcgcgctaa aaaaatccgg gatcaaggaa tcggaagtca
     3841 cgatcctaca gatggccggc acgccgggag tgtcgcaggc gctggaagcg ggccggatcg
     3901 aggtcggcgt tcttggagag tcgggtgccc tcctcgtctt ccgtggaaag gcgcgttttc
     3961 tcaaaggggc gagctctaga gaatttggct ttcatgctct ggatgccccg ctcggaacga
     4021 ccgagcgcaa gatcaaagcc gatcgaggtt cagtgctgag gttcatgcaa gcttatgtcg
     4081 aggcacttca ttacttcaaa accaatcgag cagggacgat aaaaattctc cagaaatata
     4141 tggccggttt aagcgaagag catgtcgcgc tctggtacga tgagtatcgt gaaggtttgg
     4201 tgtcgttgcc ttacccggat gacaacgggt tgcgcgcgga attggatctt atcaacgtgc
     4261 cgcgctccca gtcgctggcg ccgtatgtga acaagagttt cttggacgag atcaagaaga
     4321 gcggactggt agaacggcta tacaaataat ggaggaaaga gccatggcta agatcagaca
     4381 tctcgcaatc aaagcggaag atcaggaacg agctgcaacg ttttataaag aaaccttcgg
     4441 catgaccgaa gcctggcgcg gcccggtgcg agaagacggc catcgtgcga tctacctgac
     4501 cgatggttat atcaatatgg cgatcctgcc ggcgcgcggc gggcgtgagg gaatcgatca
     4561 ctttggtttt caggtcgaag atctcgaaag cactctaaaa accgcggcat cgacaggcgc
     4621 gaaaagcgat tcatacaaaa aaccgcgcga tggtagattc gccgaagtgg gcgtgcagga
     4681 cccggtgggc actccggtgg atgtttccgt gcacggctgg aaagcgtaaa catcagcgag
     4741 gattaccgtg gataaaacta ctttgaagaa ggtatccgtt acgcgaggtc ggtggatgaa
     4801 gaagacaagt tggttgatca tcgttttatt ctacaatttg gtgatgcatg cctctgcgag
     4861 ctttgccgcc gaaccttcga agctactgat cgcgcacggt gccatcagta ataacgttgc
     4921 gccgttgtgg attgccaaag agcagggcat ttttcgcaag tacggcatag aggcggatct
     4981 ggtatttatc atcgccgggc gagcgatgca ggcgatgctg gcgggacagg tgccggtggg
     5041 cctggtcggg gggacccatg tgaccaacgc caacactggc ggcggggact tcacgatgat
     5101 cctcgggctg gagaacaagc tcgattacat tttcgtggcc aagccgacga tcaaaggcgc
     5161 cgaggatttg aagggaaaga aagtcgcgat cggtacgcct gccggttcag catctttggc
     5221 gacatacgtg tcactcgacc atctcgggct cgtgccgcgg cgtgaccaaa tcactttgct
     5281 gggcatcggc ggcgtgcccg aacgaatgag cgcgctccgg tccggcagtg tggaagcgac
     5341 cagcttgtct cccgaaattg gtcaagtggt ggtcagcgaa gggtatcggg tcctgatcga
     5401 tttagggaaa gagggtgttc cgtttcaatc ctccgggctg gtggtgccgc gcagcttgat
     5461 caaaaccaat ccgcaattag tcgagaatat cgccaaggca accgtcgaag gcgcagcttt
     5521 tgttattaag cccgccaata aggagattgt tttaaggagc ctgggccgaa atttgcgcct
     5581 cgacaaaccc gaccgggtcg agaaagctta tcagggtttg ctggcaaatc ttccgcgtcc
     5641 accctgtccg tcgatggaag gagttagcgc aatgctcaag ctcatggcgc agcacgggct
     5701 caatgctaag gcgtcacagg taaagccaga agagatcgcc gacatgagct actgcaaaaa
     5761 attcgaggaa agcggattct tccgtagtgt gtattagcct cttctggaag tttcacgtgt
     5821 agaacggtaa acggcgagtc ctccagagcc cgccaacgga cctgtggctc ctagtctggg
     5881 caagctttgc tggtgctgcg aacgtttggg gatttttaaa ccggcctcta accccaaaga
     5941 tgactttgaa caattcgctt tatggttttg ctcttccagg ctcactttga tcgacatcga
     6001 atgggacctc caggaggaat cgaaaaaact ccgacaaaga tttacacgat gcggccagac
     6061 atggacgccg cttacgattt atatcgccag agttgaggaa tggcgatgca aatcgccgag
     6121 atcattatga aacagagaag gctccccagc agcagcgcat ttggtgctcc gacataatgc
     6181 gctgcagccc ccgctagaat cgctcccaat ccattggtcg cctgcgcgaa gaccgagatc
     6241 aggctggagg ctcgtccaag cataccatcg ggcgcctgca gttgaaccac cgtgcggcgg
     6301 atcgccacgc tcacggaatc ggtgaatcct aaaatcaaag tagcgcccac agccatccaa
     6361 aaccaatgag cgattcccaa caacgccaag ctgccggcaa agatcagcgc ggcggcgacg
     6421 accaaaacgc ctttgcgcct tatatcgccc agcataagca aggccacgga gccgacgatt
     6481 gcgcccaggg aaggggcgcc gtagagcagc cccaaacccg aggcaccggt atgaaagaca
     6541 tcggctgcaa aaatcggcag aatcggccgg tagaaaccca ctagagtaac gccaaaatcg
     6601 agaagaaaaa gcgacaatat aatacgctgc acccagatga actcgatgcc ttcaaagata
     6661 ctccgcaggt tcaccctgcg gcgtactcct tcgggcactc ccgaagtgcg catcgcaaga
     6721 attgctaata cggctggcat cagcatggca gcggcgatga aataggtcgt tactaaaccg
     6781 agctgatcga tcaagacccc gccgattgcc ggtccgagta aaaagctcat ctggatgatg
     6841 atcgtgttga gggttactgc attcatgagc aacggccgcg gcaccaatcg gggaatcaac
     6901 gccgagcgcg ccggccagtt aaaaacctcc accagcgcgc cgagaaatcc tagtgtatag
     6961 acatgccaaa cctgcacgcc gccggtgacg gtaagaagcc ccagcagcag gcttggaatg
     7021 aactggatcg acaccgttgc gatgaggagc ttcttgcgat cgaaagtgtc ggcaaccgcg
     7081 ccggcaaaca ggccgaagat tacgaaagga aaagcttgga aaaaaccagt gagcccgagt
     7141 tgaaagggag aactggagat ttcgtaaacc tgatacagac cagcgacatt ccgtacctga
     7201 accgctaccg cattgaagac gctggccaac cataccaagc ggtaatcccg aatcagcagg
     7261 gacgaccaag gccgaatgtt gctcgaagga ggtggcaact ctatttcggg tgaatcagcc
     7321 aatcggttca gaaaccaaat ggacggcgcc ggggaatagt gcgaagtggt ttgttggtgt
     7381 taagtggcgg aaagggcgct ctagcaagct gatgaaaaat actggtatct ttcgcttagc
     7441 actgctgccg ccgtacgcct cggcccgtcg aacttagcgc gcctcggctg tgagatcttt
     7501 ttgcgcggcc tgcaatccta tttttcaatc gacctgctag aaattcgccg tacccgccgc
     7561 tttgtttatc tcgaagatgc cgctacccgt atgtcttcca acacgccgcg ctcttctgct
     7621 tgcgccagca ccgcttcgat gctcttgacc agattgcgcg ctccctcgat ggttaactcc
     7681 acagccacac gcgccgccgg gcccttggcc tcgtttacga aatcgatgtt gagggcgtgt
     7741 tccagcggca tgtcgtaagg atgatcgtag gagatattgg cctcgcgcag ctcgaaccag
     7801 tcgccaccgt tcttaccgcg gccttcgatc ttcgcctgat gaacaatcat cgtgcacatt
     7861 ttccattcct ttcaattgag ccgccgcgtc cgctgctgct gctgacatgc ttagtgagtc
     7921 gatctgcgga tgtgtgccgc ggcgtgagct tctctcgagg gcttcaatca gccctttaga
     7981 tttttctcaa g
//
LOCUS       DASUTG010000936         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_442330, whole genome shotgun sequence.
ACCESSION   DASUTG010000936 DASUTG010000000
VERSION     DASUTG010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442330"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VF089_21850"
     CDS             complement(<1..440)
                     /locus_tag="VF089_21850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6176661.1"
                     /translation="MTLGALTLILLAAFIHASWNLLNKRASGHVTFTWLVAVLSAIFY
                     TPVALGVIFVTHLQIGIVELGLMAGSAALHSAYFILLNQGYRVGDLSLVYPLARGTGP
                     LLSSIAAILFLGERPSIIALAGALLIIGGAVILTTNLSQLRQTGA"
     gene            1201..4911
                     /locus_tag="VF089_21855"
     CDS             1201..4911
                     /locus_tag="VF089_21855"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6176662.1"
                     /translation="MYTALPIRSKCVEGFRYQDDFRIQLLLTALLIGVAYYVGALLSF
                     TLRVPSTRSSIIWIPNAILLAAFVVTPVKRWWLWTLAAVPAHLLAQSRDAAPVLMLLC
                     PFLANVVQAVLAAIGLRRFMDVPYRLESLREMAIFILVAVLAAPALASFSCAWLFVLA
                     GWETDYWLVAQARFLNNVVTGLTVAPLCFAIADGNLKRLPSLQIRRYVEFAILLLGLV
                     LTLFVASEWHAAETINFPVRLYAPLPFLLWAAVRFGPPGLAIALLAVAYDSISDSIFD
                     RAYFGMNSPALSVLALEASLAVLSLPLMILAALIQERQHNEEALSESQERLARTEKFS
                     LVMVTHSDLEGRWLKVPPTLCDLLGYTEQELLGRHFDELTHPDDKELNLSQRSRLLRD
                     EIKSFDLEKRYVRKDGGIVWVYINVSIVLDANGIPVHLLSYIRDITRRKQAEQGLRES
                     EERLQLALDAGGMGVWDWDTCTNTLQWSSEHFTIMGLKPFSTTPTYSIWADRVHPDDR
                     AIVSAAIERAIAERSQYRSEYRILLPDETQRWVISRGEAVYKAGSECVRLMGVTIDVT
                     DRKRAESRLNVQYQITRVLSSSASLGDAAPALIQTICESFEWHCGEIWQVSPEAEVLT
                     YLEGWHSASVPLAEFVSDSRRFTFAPGIGLAGRVWKSGAPAWINNLADDDNFPRAGLA
                     DKFGLKTAFSFPITLSDQTLSVMTFFSRKVRQTDDELLQAMESIGHQIGQFIERKKAE
                     QQTSQRDQLLHTMFESLSFHVVVLDRDGTITYASKLREQLRIHQPLLNHLAIGTNYLD
                     VCRRAASLNDNNAKEALYGIENVLAGQFPSFSMEYPCDAVEGRRWFAVQVDPMPRQHG
                     GVIISHADITQRKQADEALRNALNEVRKLKEQLEVENIYLREEVSGVHLFGEIAGRSE
                     SIAKVLRHAELVAPTDATVLITGETGTGKELLARAVHARSKRSDRPLVKVNCAGLPAS
                     LIESELFGHEKGAFTGAAAKRIGRFELANDATIFLDEVGELPLELQAKLLRVLQEGEF
                     ERLGSSKTIKVSARVIAATNRDLGLAVKAGTFRDDLYYRLNVYPIKLPPLRDRKEDIP
                     ELVQTFLREASRRLGRSLKGVSQRVMDGFMKYDWPGNVRELQNVIERMAVTAKGTQLS
                     LPSDWLSGSLTDIPNDLFKSQGDHSTVSSEEEATVENVERAHILRILEQRHWRIEGPH
                     GAAVVLGLKPSTLRSRMRKLRIDRSGHAVKYH"
     gene            5373..6425
                     /locus_tag="VF089_21860"
     CDS             5373..6425
                     /locus_tag="VF089_21860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX6176663.1"
                     /translation="MDRDIRPVGVVVVRSKYEIASTNEVFCEKLGYEKADLAGASLID
                     IAHPAEQEKCKQLLVEAVKDKDAAYQTDLSFVAKNGETVRTKLTVTTGRDQYETADIL
                     VSFEDFIGPHPRKTAPHGERLTQVEASVKIFMHELRNQMHGISLIAHCVEQESTRRGL
                     AEDDPLVTLNQDLRMRLKDIGILLQDLSRLGESIKLELQPTDLAKFMTTLLASEQASH
                     VAERIRINCYIAADVPPVQLDQRRFKQAWLNLFKNALEAMPSGGTITVRLYRTDEYVC
                     LEIEDTGTGVPDGIDIFEPSTTTKPHGTGLGLGIVREIVSAHGGLVSYQNPPHTGARF
                     TVSLPVDSPRDNSGKS"
     gene            6684..>6912
                     /locus_tag="VF089_21865"
     CDS             6684..>6912
                     /locus_tag="VF089_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6176664.1"
                     /translation="MAGDKIGFLGVSLAFGLSLLAMIYTIGPISGCHINPAVTVGLLL
                     TKKMDPKFAGGYIAAQIVGAILAAGILLVIAQ"
BASE COUNT         1618 a         1785 c         1869 g         1640 t
ORIGIN      
        1 gcgccggttt ggcgcaactg cgacaagttc gtagtcaaga tgaccgcgcc gccgatgatc
       61 agtagcgcgc cggcaagcgc gatgatagat ggccgttcgc ccagaaataa gatagcggcg
      121 atgctcgaaa gcaacggccc ggtgccgcgc gccagcggat agaccaacga caggtcgccg
      181 acccgatagc cctgattgag cagaatgaaa taggcgctat gcagtgcggc gctcccggcc
      241 atcagcccca attcaacgat gccgatctgc aaatgcgtga caaagatcac tccgagcgcc
      301 accggcgtat aaaatatcgc cgacaggaca gccacaagcc acgtaaatgt cacatgcccg
      361 ctggcccgct tatttaacaa attccaactg gcgtgaataa aagccgccaa gaggattaga
      421 gtgagcgcgc cgagagtcat gagtgttgtt aaagatggat cctgtcagag catacgaaga
      481 cgtaagagtt cattgcgtgt tcggagctga gagcctcagg cgtataggaa acgatctact
      541 ggagcaaggc agccggctca ggggcgtgaa cgatcggctt agtgccggac agtccatcct
      601 ctacctcggt tttcgcggta agatagggag agcttaacgc gctacgaaga taaaagactc
      661 aggacgacga tgtcgcgttt ttctgcccag tttctgagct catggccatc caatcgataa
      721 acttagccgg gtagaactgc caaaaaacca ttgcaaatca tatgaggcat gactagagtc
      781 aaggccttcg agattgtttt tgacgattgg cgctatagat gcagccatgc ctaacgttgg
      841 tgagtaaatg gtgataaatt gagcgcgctc tttctaacca ccagcgacct agaacgtatt
      901 atcggcagta tgtttacact tttttcgtcc ttgtcgacat tttttacatc gaactgttag
      961 cccaacgcag tctttagcag gcttattcga tgtaaagcgt gttactcgga cgttgcgaat
     1021 tgttgtagct agtaatctgc attcttcacc atcttcattt cgcactcccg gaatatcgga
     1081 gccttgatgt tttctctggt atgtatcatc gagcggctta caacctacct ttactcaggt
     1141 actacgtgaa caatacagac tcggcgcttc tatcggagtg aaggcctttc ggaggacagc
     1201 atgtataccg ccctaccaat tcgaagcaaa tgcgtagaag gattccgcta tcaagatgat
     1261 tttcgtattc agttgctgct gaccgctctt ttaatcggtg tcgcttacta cgtcggcgct
     1321 cttcttagtt tcacccttag ggttccctcc acacgtagct cgatcatttg gatacccaat
     1381 gcaatacttc tcgcggcgtt cgttgtcaca cccgtgaaaa gatggtggct ctggaccttg
     1441 gccgcggttc cggctcatct cctcgcccag tcacgcgatg ccgctcctgt attgatgtta
     1501 ctctgcccgt ttctcgccaa cgtagtccag gcggttctcg ctgcgatcgg cctgcgccgc
     1561 ttcatggatg tcccataccg gctcgagagt ctccgagaaa tggccatttt tattctcgtg
     1621 gccgtgctgg cagccccggc tctcgcctcg tttagttgtg cctggctctt cgtgctggcc
     1681 ggctgggaga ccgattattg gctcgttgca caggcacggt tcctaaataa tgttgtgacc
     1741 ggactcactg tcgcgcccct ctgtttcgcg attgcagacg gcaacctgaa acgacttcct
     1801 tctctccaga ttcggcgcta cgtagagttc gctatccttt tacttgggct cgtccttaca
     1861 ctctttgtcg cttccgagtg gcacgcagcc gaaacgatca atttcccggt tcggctctat
     1921 gctccgctgc cgtttttgct ttgggcagcg gtccgttttg gacctccagg gttagcaatc
     1981 gcccttttgg ccgtcgccta cgattccata tctgattcga tcttcgatcg agcttacttt
     2041 ggcatgaact cgccggcgct cagcgtattg gctttggagg cctcgttggc tgttctctct
     2101 cttcctctaa tgattttagc agcgctgatc caggagcggc agcacaatga ggaggctttg
     2161 agcgagagcc aagagcgcct ggcacgaact gagaagtttt cgcttgtgat ggtgacacat
     2221 agcgatctgg aaggtcgctg gctcaaggtt cctccgacgc tctgtgactt gctcggttat
     2281 accgaacaag agttgctggg gcgccacttt gatgagctaa cgcaccccga cgacaaagag
     2341 ctaaacctaa gccaacgctc gcggctcctt cgggatgaaa tcaagtcatt cgacctcgaa
     2401 aaacgctacg ttcgaaaaga tggcggcatt gtttgggtct atatcaacgt gtcgattgtg
     2461 ctggatgcaa acggaatccc tgtgcatttg cttagctaca ttcgcgacat cactcgacgc
     2521 aagcaggctg agcagggact acgggagagc gaagagcggt tgcagcttgc tctcgacgcc
     2581 ggcgggatgg gggtctggga ctgggatacc tgtacgaaca cactgcagtg gtcgagcgag
     2641 catttcacca ttatgggcct gaagccgttt agcacgacgc caacatattc gatctgggcg
     2701 gatcgggttc atccggatga tcgcgcgatt gtcagtgcgg caatcgaaag agcgatcgca
     2761 gaacgcagcc aataccgttc cgagtatcgc atacttttgc ctgatgaaac ccaacgctgg
     2821 gttatatcgc gtggcgaggc ggtgtataag gccggcagtg aatgcgtcag gctcatgggc
     2881 gtgactattg atgttaccga tcgcaaacga gctgagtcac ggttgaacgt tcaatatcag
     2941 atcacgcgcg tgctatcgag ttccgcttcc ctgggcgatg ccgcgcccgc tttgattcaa
     3001 acgatttgtg agtcttttga atggcattgc ggagagattt ggcaagtcag ccctgaggct
     3061 gaggtgttga cgtatcttga aggttggcat tcggcatcag tgccgttggc tgaattcgtg
     3121 tccgacagcc ggagatttac ttttgcaccc ggaatcggac ttgctggtcg cgtctggaaa
     3181 agcggtgcgc cggcgtggat caataaccta gccgacgatg ataatttccc acgcgcgggt
     3241 ctcgccgaca aattcggctt aaaaaccgcg ttcagttttc caatcactct gagcgatcag
     3301 acactctcag ttatgacttt tttcagccgc aaagtgcgcc agaccgacga tgaattgctc
     3361 caggcaatgg aaagtatagg acaccaaatt ggacagttta tcgaacgcaa gaaggcggaa
     3421 cagcaaacgt ctcagcgcga ccagctactc catacgatgt ttgaatcgct ttcttttcac
     3481 gtcgtcgtct tagatcgcga cggaacaata acctatgcga gcaaattacg tgagcagctc
     3541 agaatacatc agccgctgtt gaaccacctg gccattggaa ccaattattt ggacgtttgc
     3601 aggcgagccg cgagcttgaa cgacaacaac gcgaaagagg ctctttacgg aatcgaaaat
     3661 gtgcttgcgg gacaattccc cagcttttcc atggagtatc cctgcgatgc cgtcgaagga
     3721 aggcgctggt tcgctgtgca ggtcgatccc atgccgcgtc aacatggcgg cgtgatcatc
     3781 agtcatgctg acattaccca gcgcaagcaa gcggatgaag cgctgcggaa tgcgttgaac
     3841 gaagtccgca agctcaagga acagttagag gtcgagaata tctacctgcg tgaggaagtg
     3901 tccggcgttc atctattcgg cgaaattgct ggacgcagtg aatctatagc gaaggtgctg
     3961 cgacatgccg aactggtcgc cccaacagat gcgacggtcc tcattactgg agagaccgga
     4021 accggtaaag aactactcgc tcgggcagtg cacgcgcgca gcaaaagaag tgatcgacca
     4081 ttagtcaaag tgaactgcgc gggtctaccg gcttcgctca tcgaaagtga actgttcggt
     4141 catgaaaaag gcgcttttac cggcgccgca gcaaaacgca ttggccgctt tgaattggcc
     4201 aacgatgcga caatcttcct tgatgaggtc ggcgaactgc ccttggaact tcaagccaag
     4261 ctgctgcggg ttctacagga aggtgaattc gagcgcctag gcagctcaaa gactataaaa
     4321 gtaagcgcgc gagtcattgc agctaccaat cgcgacctcg ggctagcggt caaggccgga
     4381 acgtttcgcg acgatttgta ttatcgattg aacgtttatc caataaagct tccgccgttg
     4441 cgggatcgca aagaggacat accggagctt gtccagacct ttctcagaga agccagccga
     4501 agacttggtc gatctctcaa gggcgtgtca cagcgggtta tggacggttt catgaaatac
     4561 gattggccgg gtaacgtccg tgagctgcag aacgtgattg agcgcatggc ggtgacagca
     4621 aaaggcaccc agctaagcct tccctctgat tggttgagcg gttcgctgac cgacatcccg
     4681 aatgatcttt tcaagagtca gggagatcat tctactgttt catcggaaga agaagctact
     4741 gtggaaaacg tcgaacgcgc tcatatcctc aggatcctcg agcaaagaca ttggaggata
     4801 gaaggtcccc acggtgctgc tgttgttctc gggctgaaac cgagtacatt acgttcgcgc
     4861 atgcgaaagt tacggattga tcgatcgggc cacgcagtta agtatcattg aagtcctcgc
     4921 gttctcattg aatcgtcgtc cttcctgctg aatgcgtgga catcggtcgc cgcgcattcc
     4981 cccacagact gccttatcgt cgttctactt cagccgccaa tcgtcctttt ttatctgcct
     5041 gattggttaa ggccgatagc gataccgtta aatgttgtgg gagctgctga atatcgtggg
     5101 ctagaaatcc atcattccag gcgagccctt ctaagatgcg tcagaatgtc ctcgttccct
     5161 aaataaaatc aggggttatg aaatcgcgcc cggaaaatga gtgctcttcc tctgtggcgg
     5221 cattgctctt gcgctacttc attccagccg ttgcgctggg gtgtgttgtt gcgccaggtg
     5281 atggccaggg gcaaagatca ggatggtccg ctaagcgtaa gggcggaagc ttatgttcca
     5341 gacatttggt accggaaagg agaacaggtt atttggaccg cgatattcgt ccggtagggg
     5401 ttgtggtcgt gcgaagcaaa tacgaaatcg cttctaccaa tgaggtgttt tgcgaaaaac
     5461 ttggatatga aaaagctgac ctagcgggag cgagtttgat cgacattgcc catcctgccg
     5521 aacaggaaaa gtgtaagcag ctgctcgttg aagctgttaa agataaagac gcagcgtatc
     5581 agacggacct gagttttgtc gccaaaaacg gagaaacagt acgcaccaag ctcacagtca
     5641 caacgggtcg cgaccagtac gaaacggcag acatcttggt gagttttgaa gatttcatcg
     5701 gcccgcaccc ccgcaaaaca gcgccgcacg gcgagcgctt aactcaagtc gaagcatcgg
     5761 tcaaaatatt catgcatgaa ctgcgcaatc agatgcacgg tatctcgctt atagctcact
     5821 gcgtcgagca agaaagcact cgccgcggct tggccgagga cgatccgttg gtgactctta
     5881 accaagattt aagaatgcgc cttaaggata taggtatatt actgcaggac cttagtcgcc
     5941 ttggcgaatc aatcaagctt gaacttcagc cgaccgatct cgccaagttt atgactacat
     6001 tgcttgcgtc cgagcaggcc tctcatgtcg ccgagcgaat tcgtatcaac tgttacatag
     6061 ccgccgatgt gccgccggta cagctcgacc agcggagatt caaacaagcg tggctcaacc
     6121 tcttcaagaa tgcgctggaa gcgatgccat cagggggcac tatcaccgtg cggctctaca
     6181 ggacggatga gtatgtgtgt ttagagatcg aggataccgg cactggagtt cccgacggca
     6241 tcgacatttt cgagccatct acgacgacga aaccgcacgg aaccgggttg ggactgggca
     6301 tcgtgcgtga aattgtttcg gcgcacggtg gcctggtttc gtaccagaac ccgccgcaca
     6361 caggtgcgag atttacggtt tctttgccgg tggacagccc tagagacaac tccggaaaaa
     6421 gctaaagcgc atcaaccgag ctgcgaaggg ctccgcttca gctcgcgctg ggagtttctt
     6481 acgccgccgg gcgaagccaa acggacgaat gcatgaatct tctcgccaac cagtaaagtt
     6541 actgataatg attcagtcgc atcagaagga atgaaagcct cgtcgcatca agatctctat
     6601 tgggaggtga tccatgaata gatatattcc tgaattactc ggcacgtttg tgctgggtct
     6661 ttgccagttg cggcagcgcc gtcttggcgg gagataaaat aggcttcctc ggcgtctcgt
     6721 tggctttcgg cctgtctctg ctggcaatga tatatacaat tggacccatc tccggatgcc
     6781 acataaatcc tgcggtaacc gtcggactac ttctgaccaa aaagatggat ccgaagtttg
     6841 caggcgggta tatcgcggcc cagatcgtcg gtgcgatttt ggctgccgga attttgttgg
     6901 tcatcgccca ag
//
LOCUS       DASUTG010000937         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1626790, whole genome shotgun sequence.
ACCESSION   DASUTG010000937 DASUTG010000000
VERSION     DASUTG010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626790"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF089_21870"
     CDS             <1..467
                     /locus_tag="VF089_21870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176665.1"
                     /translation="VNPDLTPGTWEATVDWMKHLILPVSVLAISEIIQVQRFMRSSLL
                     EVLRQDYLTTARAKGLSEQTVIGRHALKNALIPVVTIIAVTMPRVIGGSTVVETVFAY
                     PGMGRLLYTSVMGNDYVIAMTVVMILAVAVVFFNLLADILYGWLDPRIRYQN"
     gene            471..1340
                     /locus_tag="VF089_21875"
     CDS             471..1340
                     /locus_tag="VF089_21875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6176666.1"
                     /translation="MDSTPLPIGNWRIVWRRFKQHRIAYVGFFFFVFLLLLVIAGPHL
                     LPYELDQVSLGERMHGPSTKHWFGTDELGRDTWVRIMHGGRVSLAVGLIVGLSTVILG
                     GLIGILAGYIGGALDNILMRMVDTIYTIPRIVLLLVLSKVAGPGLLNIIIILVALEWT
                     NAARLARSAVLSFREQEFVLAAHCLGASRWRVMLRHLLPNSLAPLIVAATLDAGAAIR
                     AETTLSFLGLGIQPPTPSWGNLLTNASTNIFIAPLQVFLPGIFIFLALMSFNLLGDAL
                     RDALDPRLTRRQG"
     gene            1543..1896
                     /locus_tag="VF089_21880"
     CDS             1543..1896
                     /locus_tag="VF089_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX6176667.1"
                     /translation="MKVRTKEFALAVVRLYSDLPKRREAQIIGDQVLRSGTSVGAHFR
                     EAQRTKSRPDFISKIEGGMQELEETVYWLELLEETGFATLETLTPLKNEADELMAIFV
                     TIARKTKVHLKKELI"
     gene            2068..3135
                     /locus_tag="VF089_21885"
     CDS             2068..3135
                     /locus_tag="VF089_21885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX6176668.1"
                     /translation="MPSNFVTNQEIIVQARRNLSQHVWHYLTGGAESETTMCRNRLAF
                     DSLAFRPRALVEVSKIDPSITFLGHRLRIPVMLAPIGSLQLMAPDGAVAVARAAHEFG
                     TLNFVSSVTQPSLEETAAATPHPKIFQLYVQGDIKWVETLLTRVKNAGYQALCLTVDT
                     AYYGRRERQMMDRWLPPSRRLSGYEHRAAITWETVDAIKKFAGLPFILKGVATGEDAV
                     LAVEHGVSAVYVSNHGGRQLDHNRVTLEMLPEIVEAVAGRAEVIVDGGILRGSDVLKA
                     LALGAHAVAIGKLQGWGLGAGGQQGLMRVLETLESEIITSMALLGVTRIDQLQPVHLG
                     KAQSTAPSHEMSAFAHMPRLY"
BASE COUNT          672 a          853 c          905 g          720 t
ORIGIN      
        1 tcgtcaatcc cgatctaacg ccgggcacct gggaagcgac ggtcgattgg atgaaacatc
       61 tgatcttgcc ggtgtcggtc ctggcaatct ccgagatcat tcaggtccag cggtttatgc
      121 gttcaagttt gctcgaggtg ctgcgccagg actatttgac gaccgcgcgg gccaaggggt
      181 tgagcgagca gaccgtgatc ggccgccatg ccctgaagaa cgcgctcatt cccgtggtca
      241 ccattattgc ggtaacgatg ccgcgggtaa tcggcgggtc gactgttgtc gaaactgttt
      301 ttgcctatcc cggaatgggc cggctcctct acacttcggt gatgggcaac gattatgtca
      361 ttgccatgac cgtggtgatg attctggccg tggcggtggt gttcttcaac ttattggccg
      421 acattcttta tgggtggctc gatccacgca tccgttatca aaattaactg atggattcga
      481 ccccgctccc gatagggaac tggcgcatcg tctggcggcg ctttaaacag catcgcatcg
      541 cctatgtggg attttttttc tttgtttttc ttttgcttct ggtgatcgcc ggaccgcatc
      601 ttcttcccta tgagcttgat caagtttcgc tcggagagcg catgcatgga ccgtcgacga
      661 agcactggtt cggcaccgat gaactcgggc gcgatacctg ggtccggatc atgcatggcg
      721 gccgagtctc gctcgcagtc ggcctgatcg ttgggttgag cacggtaatc ctgggcgggc
      781 ttatcggaat tctcgcgggc tatatcggtg gagctctcga caacatcctg atgcgcatgg
      841 tcgatactat ctatacgatc ccgcgcatcg tgctgctgct ggtgctgtcg aaagttgccg
      901 gaccgggcct tctgaacatc atcatcattc tggtcgcgtt ggaatggacc aatgcggccc
      961 gcctggcgcg cagcgcggtg ctttcttttc gcgagcagga gttcgtgctc gccgcccatt
     1021 gcctgggcgc gtcgcgctgg cgcgtcatgc tgcgccatct gctgccaaac tcgctggcgc
     1081 ccctgatcgt cgcggccacc ctcgatgccg gcgctgccat ccgcgccgaa acaaccttga
     1141 gctttctcgg gcttggcatc cagccgccga cgcctagctg gggaaatctc ctcaccaacg
     1201 catcgacgaa tatcttcatt gctcccctgc aagtgttcct accgggaatc tttatctttc
     1261 ttgccctgat gagcttcaac cttctgggtg acgcgctacg ggatgcgctg gatccgagat
     1321 tgactagaag acaaggatga aggatgaaag cggaaaactg aaatagaccc ggggataagc
     1381 tcacatggga accgaaatgc aaaatcgagg ctaaacgttg aagctaaaca caatggagat
     1441 gtgtcaaacg aggagatgaa caccggaagg acgaaagctg cggcgtgaaa gcatgcgtaa
     1501 gggacactga tgagttttga aaacggaagg atgagaggag acttgaaagt taggacgaag
     1561 gaatttgcgc tggcagttgt tcggctatac tccgacttgc ccaagagacg cgaagctcag
     1621 atcatcggag accaggtgtt aagatcaggg acctctgtcg gagcgcactt ccgagaagca
     1681 cagcggacga aatcaagacc agacttcata agcaaaatcg aaggtggcat gcaggaattg
     1741 gaagagacag tctactggct cgagctgctc gaagagaccg gatttgcgac gctagaaaca
     1801 ttgacgccac tgaaaaatga agccgacgaa ttgatggcga tctttgttac gattgctagg
     1861 aaaacgaaag ttcatttgaa aaaggagctg atttaacttt tcattggcta gagtcgcaaa
     1921 tatgccgcaa tattacgatt cccgcctgag taagctttca tcgctgttta attttcgctt
     1981 ttcatcattc atcagtaata gggttcattt cagctttccg ctttcagctt tcataattca
     2041 tcattacgcc ctcaaacagg agcccttatg ccctccaatt tcgtcaccaa tcaggaaatt
     2101 atcgttcagg cgcggcgcaa tctgagccag catgtctggc actatctgac cggcggggcg
     2161 gagtccgaaa ccaccatgtg ccggaatcgg cttgcgttcg atagtctcgc ctttcggccg
     2221 cgcgcgcttg tcgaggtctc gaagatcgat ccatcaataa cgtttcttgg acatcggctc
     2281 agaattccgg tgatgctcgc gcccatcggg tcgctgcagc tcatggctcc tgacggcgcc
     2341 gtggcggttg ccagggcggc gcacgagttc ggcacgctca actttgtcag ctcggtcacc
     2401 cagccgagcc tcgaggagac cgccgccgcg acgccacatc caaaaatctt tcagctttat
     2461 gttcagggcg atatcaagtg ggtcgaaact cttctgacgc gcgtcaagaa cgccggttac
     2521 caagccctct gtttgacggt cgataccgcc tactacggca ggcgcgagcg ccagatgatg
     2581 gatcgctggc tgccgccgag ccggcggcta tcgggctacg aacaccgcgc agcgatcact
     2641 tgggagactg tggatgcaat caaaaaattc gccggcctgc cgtttattct caagggagtc
     2701 gccaccggcg aagatgccgt gcttgccgtc gagcacggcg tcagcgcggt gtatgtctcc
     2761 aatcacggcg ggcgacaact cgatcacaat cgggtaactc tcgagatgct gccggaaatc
     2821 gtcgaagctg tagccggcag ggcggaagtg attgtcgatg gcggcattct gcgcggcagc
     2881 gatgtgttga aggcgcttgc gcttggcgcc cacgcggtgg ccatcggcaa actccagggt
     2941 tggggccttg gagcgggggg ccagcagggt ctgatgcgag tactggaaac tctcgagagc
     3001 gagatcatta cctcgatggc acttctcggc gtaacccgca tcgatcaatt acagcccgtc
     3061 cacttaggta aggcccagtc tactgcgccg agccatgaga tgagcgcgtt tgcgcacatg
     3121 ccgagattat attagagcgg cggttcctct
//
LOCUS       DASUTG010000938         6143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1037533, whole genome shotgun sequence.
ACCESSION   DASUTG010000938 DASUTG010000000
VERSION     DASUTG010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037533"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF089_21890"
     CDS             <1..190
                     /locus_tag="VF089_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176669.1"
                     /translation="NQALKPILLHQLQPGARIISYTFDMGDWPPKIAESYRDGAGDVH
                     TLYYWEIPQSVAHTENSH"
     gene            complement(232..1065)
                     /locus_tag="VF089_21895"
     CDS             complement(232..1065)
                     /locus_tag="VF089_21895"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6176670.1"
                     /translation="MEKQYTRETLLISAEALKDKLGSDNLCLIDVRAAEDFAQGHIRG
                     AVHFDLFGLSLVDTSEAPLKAFMHMIHHIFELRGVSENKEVVVYENNSGMRAARALWF
                     LDYFGHPNARMLDGGMQAWKAAGFTTTTEVTSPKGAAFKISERRQVLATADDVLQSLE
                     KKDVSILDTRSRAEYVGRQIRAARGGAIPGAIHIEWTDNLDASGKFKPDAELQAMYSR
                     AGITPDTEVISYCQGGYRAAHSYVALRLIGFPKIRNYIGSWKEWGDRADLPIDQPAEK
                     K"
     gene            1113..2249
                     /locus_tag="VF089_21900"
     CDS             1113..2249
                     /locus_tag="VF089_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX6176671.1"
                     /translation="MKAKRIFHGIFVLILLPIFTISPPVKGRAQSEKTAPFSYRLALP
                     GRTLSFPADHYSHPDFKTEWWYYTGHLQSESGRRFGYQVTFFRFGVRDRQASIQEKPP
                     LFTELYMAHFALSNISGRDFLFRERINRGIGDRAGAKTDRFFVWNEDWSVEGDRDNHK
                     ISVNDRGTELRLELKSLKPPVLHGQNGYSQKGEGEGRASYYYSLTRMQAEGEIVAGGK
                     REKVRGLSWMDHEFGSNQLGEDQLGWDWFSIQLENQSELMLYLMRRKDGSIDPYSHGT
                     VVYTDGTTRHLALTDYRVDVRARWQSPKSGAEYPIKWRVIIPAEQIELELAPHFPEQE
                     LITNRSTRVTYWEGAVQVKGTLRTKPIAGSGYVEMTGYAGKVKF"
     gene            complement(2365..2610)
                     /locus_tag="VF089_21905"
     CDS             complement(2365..2610)
                     /locus_tag="VF089_21905"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6176672.1"
                     /translation="METWRPEEIGATAGKVWNFLHKRGESSLSAIERGVGAPKQLVCM
                     AIGWLAREGKVALRQEKRSLQLCLTDGEGAQNSRMQQ"
     gene            2881..4629
                     /locus_tag="VF089_21910"
     CDS             2881..4629
                     /locus_tag="VF089_21910"
                     /inference="COORDINATES: protein motif:HMM:NF006203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX6176673.1"
                     /translation="MSNDKTKFVTVSTGAEAFLAQVRALGAVRYLFANTGTDHGPIIE
                     ALAKTANEDPNDLQPIVVPHELAAVSMAHGYYNVTQKPQMVLVHTLPGTANALGGIIN
                     AQSTNVPVLLVAGRTPITEGELRGGKSQNIHWRQESRDQGNIVREFVKWDYEVRTNQN
                     LAAVVSRAFKIAMSEPRGPVYLTLPREWLCEALESTELLSDCLTPSSKMQADYAALED
                     AAELLMSAEHPLIVTKYLGRNPEAVPLLVELAELLAIPVVQQMSYLNFPTDHPLYLGV
                     QTIQHSKEADVLFFIDTDVPWEPPSRRFIRPDSKIIHLERDPLFTGIPGWGFPADLPI
                     TGCSEIALPALTSIIKSRLQKSNTKSNRFEERRNKIKGKHDAMWRQIEASIDAVKNEK
                     PISPLWVSKCIGDVIDDNTIIVNETVTSKLADVIHLNRPGSQFGTPLAGHLGWGLGAA
                     IGVKLGAPEAIVIAAEGDGSYMFCAPTACHFTAQKYRIPFLTVVYNNQAWNASLNAAR
                     GLFPDGVAQKTGNFPGTDLTPSPKFELTAQACGAYAERVEAPEALPGALERALKVVRE
                     EKRQAMLNVICKDPLR"
     gene            5011..5568
                     /locus_tag="VF089_21915"
     CDS             5011..5568
                     /locus_tag="VF089_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX6176674.1"
                     /translation="MLSSEEFNDLSSRRNEPSLGMVFLLVSSLMQGGSVLPGSPVGFK
                     PQSAEAATLDDAVPVKTNRTQGKATRQANSRETSKRKIIRGQASWYGPGFHGKKTASG
                     EIFDQSRLTAAHKTLPLGTKAKVTNLENGNTVEVEINDRGPYVGDRVIDLSRAAANAL
                     GFVESGLTLVRIEPLYQEGSAEEAG"
     gene            5648..5815
                     /locus_tag="VF089_21920"
     CDS             5648..5815
                     /locus_tag="VF089_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX6176675.1"
                     /translation="MLSWSITFLVIGLIAGLLGLSGVAGAATQIAWVLFVIFLVLFLV
                     SLVMGRRSPPV"
BASE COUNT         1415 a         1615 c         1651 g         1462 t
ORIGIN      
        1 aaatcaagcg ctcaagccga tcttactgca tcaactccag cccggcgcgc ggattatttc
       61 gtacaccttc gacatgggcg actggccgcc gaagattgcc gagtcctatc gggatggagc
      121 gggcgacgtg cacacgttgt actattggga aataccgcaa tcagtagccc atactgaaaa
      181 ctcgcactaa atgggtcttg cctatttcat tctgccgctt agtcgcttga tttacttctt
      241 ctccgccggt tggtcgatcg gcagatcggc gcggtcaccc cattctttcc aggaaccgat
      301 gtagttgcgg attttgggaa aaccgattaa tcgcagcgcc acgtaactgt gcgcggcgcg
      361 ataccctccc tggcagtacg agatcacttc ggtgtccggc gttattccag cccggctgta
      421 catggcttgc agctcggcat cgggtttgaa tttgccgctg gcatccaggt tatcggtcca
      481 ttcgatgtga atcgcgccag gaatggctcc gcctcgggcg gcacgaatct gcctgccgac
      541 gtattcggcg cggctgcgag tgtcgaggat tgagacgtct tttttttcta gcgattgaag
      601 gacgtcgtct gccgtagcta gcacttggcg ccgttcgctg attttgaaag cggcaccttt
      661 gggggaagtt acttcagtgg tcgtcgtaaa tcccgccgct ttccatgcct gcatgccgcc
      721 gtcgagcatg cgcgcattgg gatggccgaa gtagtctagg aaccagagcg cccgtgcggc
      781 gcgcatcccg gagttattct catagacgac cacttctttg ttttcgctaa cgccgcgcaa
      841 ttcgaaaatg tgatggatca tgtgcatgaa agctttcagc ggcgcctcac tcgtatcaac
      901 gagactaaga ccgaacaaat caaaatgaac cgcgccacgt atgtgcccct gagcgaaatc
      961 ctcggccgct cgcacatcga tcaaacaaag attgtcagag cccaatttgt cctttagtgc
     1021 ctcggccgat atcaggagcg tttctcgtgt gtattgtttt tccatagtct ccctatagca
     1081 ttagtcaaaa agaatccgca attgtgataa cgatgaaagc caagagaatt ttccacggca
     1141 tatttgttct aatcctgctc cccattttta ccatctctcc tccggtgaaa ggtcgggctc
     1201 agagtgaaaa gaccgctccc ttcagttatc gactcgcgct acccggcaga acgctgtcat
     1261 ttcctgccga tcactattca catcccgatt tcaagaccga atggtggtac tacaccggtc
     1321 accttcaatc ggaaagcggc cggcgcttcg gttatcaagt gacattcttt cgatttggcg
     1381 tccgggatcg tcaggccagt atccaagaga aaccgccgct gtttaccgaa ttgtacatgg
     1441 cgcatttcgc tctttcgaac atctcaggaa gggattttct ttttcgcgag cgcatcaatc
     1501 gcggcattgg cgacagggca ggcgccaaga ccgaccgctt ctttgtctgg aacgaagact
     1561 ggtctgtgga aggcgatcga gataatcata aaatttcagt gaacgaccgc ggcaccgaac
     1621 tccggcttga gctaaagtcg ctcaagccac cggtcctgca tggtcaaaat ggatacagcc
     1681 aaaaaggcga aggggaaggc cgggcgtcgt actattattc gttgacacgg atgcaggcag
     1741 aaggagaaat agtagccggt ggcaagcggg aaaaggttcg cggcttgagc tggatggatc
     1801 acgaatttgg cagcaaccag ctcggagaag atcagctcgg ctgggactgg ttcagtattc
     1861 aactcgagaa ccaaagcgaa ctcatgcttt atctcatgcg ccggaaggat ggctccatcg
     1921 atccgtactc acatggaact gttgtgtaca ccgatggaac aacacggcat ctggctctga
     1981 cggactatcg agttgatgtc cgcgcgcgct ggcaaagtcc gaagagtggt gcggagtatc
     2041 caataaagtg gagagtgatt atcccggctg agcaaatcga gttagagctc gctccgcact
     2101 tccctgagca agaactgatc acgaatagaa gcacgcgggt tacttactgg gagggcgcgg
     2161 tgcaagttaa aggaacgctg cgaaccaagc ccatcgccgg ctcgggctac gtcgaaatga
     2221 ccggatacgc aggtaaggtg aagttctaac ggtctaggag taagtcagcg gatgcccaaa
     2281 gccttacgat tttggatgtc ttactttttt gtcgaggaaa ttattctaac gtgtgcatct
     2341 ctccgggtat gaacccgacg ctctttattg ctgcatgcga gagttttgcg cgccctcgcc
     2401 gtccgtcagg cacaattgca acgagcgctt ttcctgccgt aatgcaactt tcccttcccg
     2461 cgcaagccag ccaatggcca tgcataccag ctgtttcggg gcgcccactc cgcgttctat
     2521 cgcgctgagg cttgattcgc cgcgcttatg cagaaagttc cataccttgc ccgcagtggc
     2581 accgatctct tccggtctcc acgtctccat ttgtccttcc tcctggtaac ttttgaggct
     2641 gaaatgcatc atgcttggat cgtttcaatg tttcatttat caaaactttg tcaagttcgt
     2701 ttgcccaccg tgggacgcga atgtgcgctg gatcgaggta gccgtacgtt gaaatagtgc
     2761 gggcttgacg catttgaaac atcccatgta atctgcggac agctgtgcat ggatgccaga
     2821 gatttctgac tgtcggttca ccgtcgggtt tcctcgccga tgggaccaaa ggaatcattc
     2881 atgagtaacg acaaaacaaa atttgtaacc gtgtccaccg gggccgaggc cttcctggcg
     2941 caagtgcgcg cgcttggggc cgttcgttat ctcttcgcca ataccggaac cgatcacggt
     3001 ccgatcatag aagcgttggc aaaaactgcg aacgaagatc ctaacgatct tcagcccatc
     3061 gtcgtaccgc acgaactggc ggcggtctcc atggcgcatg gttattacaa cgtaactcag
     3121 aagccgcaaa tggtcttggt gcataccttg ccaggcaccg ccaatgccct aggtgggatc
     3181 atcaatgccc agtccacaaa tgttccggtg ctgctggtgg cgggcagaac accgatcacc
     3241 gaaggggagt tgcgtggtgg caaaagccaa aacattcatt ggcgacagga gtcgcgcgat
     3301 caaggaaaca tcgtgcgcga gttcgtcaaa tgggattacg aggtgagaac caaccagaat
     3361 ctggccgcgg tggtctcgcg cgccttcaag atcgcgatga gcgagccgcg cggtccggtg
     3421 tatctgacct tgccacgcga atggttgtgc gaagcgttgg aatcgacgga gttgttatcg
     3481 gattgtttga cgccctccag caagatgcag gcagactacg ccgcattaga ggacgccgcc
     3541 gagcttctga tgagcgcgga acatccgcta atcgtgacga agtatctcgg gcgcaatccc
     3601 gaagcggtgc cgctactcgt agaacttgcc gagctactcg caatcccggt cgtgcaacaa
     3661 atgagttatc tcaactttcc gaccgatcat ccgctctacc tcggagtgca aacgatccag
     3721 cattccaaag aagccgatgt gctttttttc atcgatacgg atgtgccgtg ggagccgccg
     3781 agccggcgtt ttatccggcc cgactcaaag atcattcatc tcgagcgtga tccgctgttt
     3841 accggtattc ctggatgggg cttccctgcc gatttgccga taacgggctg ctcggaaata
     3901 gcgctgccgg cgctgacgtc gatcatcaag tcgaggctgc aaaagagcaa caccaaatct
     3961 aatcgcttcg aggaacgccg caacaaaata aaaggcaagc atgacgccat gtggcgtcaa
     4021 atcgaggcga gtatcgacgc agttaaaaat gaaaagccga tcagtccatt gtgggtatcg
     4081 aagtgtatcg gtgacgtaat cgacgataac acaatcatcg tcaacgaaac cgtgacctcc
     4141 aaacttgccg atgtcattca tttgaaccgc ccaggttctc aatttggtac gccactcgcg
     4201 gggcatctcg gttggggtct cggagcggcc atcggtgtaa agttgggagc tcctgaagca
     4261 atcgttattg ctgcggaagg agatggctcc tatatgttct gcgcgccaac tgcctgtcat
     4321 tttaccgcgc agaaatatcg cattccgttt ttaacggtgg tctacaacaa ccaagcttgg
     4381 aacgcgagcc tcaatgccgc gcgcgggctc tttcctgacg gtgtcgccca aaaaaccggc
     4441 aactttccag gcacggattt aactccgtcg ccaaagttcg aattgacggc gcaagcttgc
     4501 ggcgcctacg cagagcgtgt cgaagcaccg gaagcgcttc cgggcgcact agagcgcgcg
     4561 ttaaaagtcg taagagagga aaagcgccag gccatgctca acgtcatttg caaagatcct
     4621 ttgcggtaaa atccgtaatt ccgcaccgat tctgtctcac cttcaagaca tctcccatcc
     4681 aacgcgggtt aaaagccgag ttttggttcc taacgattga taaacccaca aacttgctgt
     4741 gccaactttg ctcaccggac tgggcttgca tagcaaaaga tcattcccca tagcgacaac
     4801 gtggcccggg gaggcatacg cttgtaggtt attcagtaca tggattgcgc cggtctttga
     4861 tcgaccctac tacgatactc ctttgtttct aatgacttag aggctttttt ccgccaaccg
     4921 gcacggctct tgccttactg ttcgttcaag gtgagcgact cggatcatga catgatcgct
     4981 actggattca tcataagagg gagggaagag atgctttcgt ccgaagaatt caacgatttg
     5041 tcttctcgtc gtaacgagcc ttcgttgggc atggtctttt tactggtatc ttcactgatg
     5101 cagggcggca gtgtgctgcc aggatctccg gtcggtttta agccgcagtc cgcggaagcg
     5161 gcgaccctgg acgatgctgt cccagtgaaa actaaccgga ctcaaggaaa agctacgcga
     5221 caagcaaata gtcgtgagac ttcgaagaga aaaataatcc gggggcaagc ttcctggtat
     5281 gggccaggat ttcacgggaa gaaaaccgcc agcggagaaa tttttgatca gagccggctg
     5341 acggcggcgc acaagacgct tcccttaggc acgaaggcaa aagttacaaa tttagagaat
     5401 ggcaacactg tagaagtaga gatcaatgat cgcggaccct acgtgggcga tcgggtgatc
     5461 gatctctcgc gtgccgcggc taacgccttg ggctttgtcg agtcgggcct aacactagtt
     5521 cggattgagc cgctgtatca ggaaggtagc gcggaagagg cgggctgatg tgccaaagtc
     5581 tgtattgtcg cctagaagtg tcggtgtgtt gtgctgcaga ggagaaacca aataggagga
     5641 gaactttatg cttagctgga gtattacatt tctagttatt ggtctaatcg ctggtcttct
     5701 gggtctatcc ggtgttgccg gcgcggcaac tcaaatagcc tgggttctgt tcgtgatctt
     5761 cttggttctg tttttagtga gcttggtcat gggcaggcgt tcgccgccgg tctgatcaga
     5821 cttaccatcg ggcagtttat tcaggcttgg gtccggcggc tcctagtagc ctccggaccc
     5881 tgtcatttgt aggagtaaat agctcatcga tagatcatct ctttatgtcc gctcttgctg
     5941 attacacacg cgcctaactc tacctagaac ttcaaatcac ctccggaata acgtgctact
     6001 gaattcatcg ttgatttagg cgtgattact tgcacgatgc tttattcaag gcaccgctcg
     6061 cgagcacttg aggaagttgt cagctgtaag cagactcgac cgtctccgat gagactccgt
     6121 gcgatgttag cggcagagtt gaa
//
LOCUS       DASUTG010000939         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1331465, whole genome shotgun sequence.
ACCESSION   DASUTG010000939 DASUTG010000000
VERSION     DASUTG010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331465"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..87
                     /locus_tag="VF089_21925"
     CDS             <1..87
                     /locus_tag="VF089_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX6176676.1"
                     /translation="ERIMMYLTGLKNIRDVIPFPRTPGNADF"
     gene            complement(232..2079)
                     /locus_tag="VF089_21930"
     CDS             complement(232..2079)
                     /locus_tag="VF089_21930"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6176677.1"
                     /translation="MPEDRSFATSRIGRTVGKVSPFTVAATVLVIVAFYWAQAVLIPF
                     ALAILLTFLLSPIVTRIQQAGLGRGTSVTIVIALTFAVLAMIGWATVSQVTSLAEELP
                     KYRQNIRQKVRDLRGMGKGGVLEQVQETVDEVKNEMEQGETSPAANKRQPIIVQSEPA
                     VSWRSLYLGPLIQPIASATLVLGLLIFMLAQREDLRNRLIRLIGYAHLTVTTKALEEA
                     GQRISRYLLMQITINGCFGIIISVALFLIGLPYAFLWGFLSVPLLFIPIIGFWTAAAL
                     PTILSMGVFTDWWWPLLVVGLFLVLKTIINMLLEPLLYGRSIGVSPVPLLMMIAFWTW
                     LWGPVGLLLATPLTVCLLVFAKHVPQLEYVEVLLSDEPAMEIQVSFYQRLLAMDQVEA
                     IEILKNFLKDHKHEQVYDELLLPALSYAKEDRRRNNLNDHEELFLLQALREIIEIAGA
                     DLQQSILSENGPTASVNSDPLPWIKIIGCPAHGEADEVALLMLRQLLVSRNCSMEVLS
                     STALAAEVIVRVREDNPALVCIAAVAPAGLVQVRYLSKRLRASFPDLRLVIGRWGLME
                     ADENGGSLPEDSGEVVGSTLLQTRDQITNLRQLISDVEPETRSEVLPAT"
     gene            2451..3683
                     /locus_tag="VF089_21935"
     CDS             2451..3683
                     /locus_tag="VF089_21935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6176678.1"
                     /translation="MAPRLRLTPTARGLITLYASTLLSGMWSMLVPSIPVMAVHFGIS
                     PGTAAQIVTALAFGRLAGMPISGVVLDRLGTRTALVAGPGLACAVALLAAIVPWFSII
                     LLLLFVLGIAESIWVIAREVAGIDLALPNQRGRVLSGFHGVNYLGLALGPLAGGFLAE
                     ASGFRGAFIAYAICAAISVFLGFTVENSRTPHNLQATATVRGQSITAWLQRWRNMRDL
                     VYQIPPHFRATYVVLVFATFTSFMHRVTTQSMLPLFATTELGLSPKEVGLLFTISGIA
                     AFAMILPAGFLIDKVGRKWATVPSTGIPALAFLLIPFAGSFFGLALLMLFLGIANGLS
                     LGSLATSTFDVIPVNARGRLQALRRTIAELGGVGAPLAGGVLADSFNPGVPFLVYAPL
                     IILSAVLLALIARETLQR"
BASE COUNT          926 a         1092 c         1010 g          929 t
ORIGIN      
        1 gagcgcatca tgatgtatct caccggactg aaaaacatcc gcgatgtcat ccccttcccg
       61 cgcacgccgg gcaacgcgga tttctgatcc cagatctggc gcaagcgccg tacagctcat
      121 tgcaaggggt aaatagccac accgctaaca cctaacgagc tggaattatt agctacagtg
      181 ctctaatcgg acggttgatc caagagagag caagattccg agccagtagt cctaggtggc
      241 aggcagcact tcgctgcggg tttctggttc gacatccgaa ataagctggc gcaaattggt
      301 gatctgatca cgtgtctgca gcagagttga gcccactacc tcgcctgagt cttcgggtag
      361 cgaaccgcca ttttcatccg cctccatgag tccccatcgg ccgatcacaa gcctgagatc
      421 gggaaacgac gcacgcaggc gcttcgataa atagcgcacc tgaaccaatc cggcgggagc
      481 gacggctgca atacaaacta aagctggatt gtcttccctc acccggacta ttacttccgc
      541 agccaaagca gttgacgaaa ggacctccat tgaacagttt cttgaaacca gtaactggcg
      601 cagcatcaaa agggcgacct catcggcctc gccatgcgcc gggcagccga tgatcttgat
      661 ccatggcaat gggtcgctat taaccgacgc tgttgggccg ttctcagata gtatggattg
      721 ctgaaggtcg gctccggcga tctcgataat ctctcgcaac gcttgcaaca aaaagagctc
      781 ctcatgatcg tttagattat tacgccggcg atcctccttg gcataactca aagctggcag
      841 gagcaattca tcataaacct gttcgtgctt atgatctttc agaaaattct tgagaatttc
      901 gatcgcctcg acctgatcca tggctagtaa tcgttgatag aaactgactt ggatttccat
      961 cgccggctca tcgctcaaca gcacttcgac atattcaagc tgcggaacat gcttggcaaa
     1021 taccagcaga cagactgtta agggcgttgc cagcagaagt cctaccggtc cccatagcca
     1081 ggtccaaaaa gcgatcatca tcaggagcgg cactggcgag acgccgatac tcctgccgta
     1141 cagcaacggt tcgagcagca tgttgatgat ggttttcaag accagaaata gcccaaccac
     1201 gagcagcggc caccaccaat cggtgaaaac acccatactc aaaatcgtcg gcagtgccgc
     1261 tgccgtccaa aatccgatga tcggaataaa cagcaacgga accgataaga atccccagag
     1321 aaatgcgtag ggcagaccga tgagaaaaag cgctacacta atgatgatgc caaagcagcc
     1381 attgatcgtt atctgcatca gcaagtagcg gctgatgcgc tggcccgcct cttccaatgc
     1441 cttggtcgta accgtcaagt gcgcatagcc tataaggcgg atcaaccgat tgcgcaggtc
     1501 ctcgcgttga gcaagcatga atatcaataa tcccaacacc agagtggcgc tggcgatcgg
     1561 ttgaatcaac ggtccaaggt aaagcgagcg ccaggaaacg gctggttcag attgaacgat
     1621 aatcggctgc cgcttattag ccgccggaga agtttcaccc tgctccatct catttttgac
     1681 ttcgtctaca gtttcctgta cctgctccag cacgccgcct tttcccattc ctcgtagatc
     1741 ccgaaccttc tggcggatgt tctgtcgata tttgggcagt tcttcggcaa gacttgtaac
     1801 ttgagaaact gtagcccagc cgatcatcgc cagcacggcg aaagtaagag cgataacgat
     1861 cgtgaccgac gtgccgcgcc cgagacctgc ctgttggatt ctggtaacga ttgggctgag
     1921 caaaaaagtc aacagaattg ccaacgcaaa cgggatcagc accgcttggg cccagtagaa
     1981 agcaacaatc accaggaccg tcgcagcaac ggtgaaggga cttaccttgc caacagtgcg
     2041 gccaatgcgg cttgtcgcaa acgaacgatc ttccggcaat ttacttcctt cggcgcctat
     2101 tgcgtgtctg actcagctgg tcagcggact gtttttttcc atactgatct atctatttta
     2161 gtcacgcata atgactgcgt tatacaacca agcgcggctc gggctcaatc atacgcgcag
     2221 gctctcaccg catgagcgat ggatagatcc cttcagaggg tctgtgctaa cttcacccga
     2281 gtagtttatt acctctccaa ttcatcagga tactcacaaa ccacatgaag agagaagaag
     2341 atagactaaa tttaaccatt tatcactggc gctcttggta tcacttgggg tggcagactt
     2401 ttgagacaac ttgacttgcg gcactagctt cggaccttaa gatcaaacat atggcgcctc
     2461 gactaagact tacgcccacc gctcgcgggt tgattacgct ctatgcaagc acactgcttt
     2521 ccggaatgtg gtctatgctc gtgccgagta ttcccgttat ggcggttcat ttcggcatca
     2581 gtccagggac cgccgctcaa atcgtcactg ctttggcttt cggcaggcta gcgggcatgc
     2641 ccatcagcgg agtcgttctc gatcgcctcg gcacgcgtac cgcgctggtt gccggacctg
     2701 gactggcgtg cgccgtcgcc cttttggccg cgatcgtgcc gtggttcagt atcattctgc
     2761 tattgctttt tgttctcggc attgccgaaa gtatctgggt tatcgcgcgc gaggtagccg
     2821 gcatcgatct ggcgcttccc aaccaacgcg gacgagtcct cagtgggttt catggagtta
     2881 attatttggg gttggcgttg ggtccgttag ccggaggttt cctggctgaa gccagcggct
     2941 ttcggggagc atttattgcc tacgccatat gcgcggcaat atcggtattc cttggtttca
     3001 cggttgaaaa ttcgcgaact ccccacaacc ttcaagccac cgcgacggtt aggggccaga
     3061 gcataactgc ttggctgcag cggtggcgca acatgagaga cttggtttat cagattccac
     3121 cacattttcg agcgacttac gttgtcctgg tatttgccac tttcaccagt ttcatgcacc
     3181 gggtcacgac ccaaagtatg ttgccgctgt tcgcgactac tgagctcggc ctatcaccca
     3241 aggaggtagg cctattgttc acaatctcgg ggattgccgc ctttgcgatg attctccctg
     3301 caggttttct catcgacaaa gtgggccgta agtgggcgac tgtgccgagc accggaatcc
     3361 cagccctggc ctttctgctc atcccatttg ccggcagttt tttcggatta gctctactca
     3421 tgttgtttct cggcatcgcc aatggtttat cgttaggctc gctagccacc tccacttttg
     3481 acgttatccc tgtaaatgcc cgaggacgct tacaagcact tcgacgaacc atcgccgaac
     3541 tcggcggcgt cggcgcgcct ttggcaggag gcgttctggc agacagtttc aatcccggag
     3601 tgccgtttct cgtctatgct ccgctgatca tcctatcggc tgtgctgctg gcgttgatcg
     3661 cgcgtgaaac gctgcagaga tagatcggtg acgagaatcg caggacaccc aaatgatgac
     3721 aacaagccgg acaagcacat gagttttggg atattcctaa aagggtgctg aaaaaggctc
     3781 ttgcaggctg cttaaaaaga tccagatgcg aggcgcgcga aaaatcgacg agcggaggcg
     3841 ttcttacagt acgttgcagc gaggcgattg agcggcaact aagcatatgg gtctttttca
     3901 cagcctgcta gcttagaatc catatttttc ttgagccgcg actgctatgc cgaaggc
//
LOCUS       DASUTG010000940         4315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1628089, whole genome shotgun sequence.
ACCESSION   DASUTG010000940 DASUTG010000000
VERSION     DASUTG010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628089"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VF089_21940"
     CDS             <1..671
                     /locus_tag="VF089_21940"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6176679.1"
                     /translation="VPGGQAVQRADDFVAVRTGQIVFISNESARAVMAAYWFRQMGFA
                     HSAVLQGGLRAWTQGGVEIEKGSLRNDPVCLEAARQAVRVVDPSTIEGKTRDPSVLLL
                     DVGTSLDFETAHVPGAKWISRGWLDLRLPETYPDRNQPIVMTCADGRQSLLAALALGE
                     LAYSDVVVIHGGVRAWSAAGFPTEQGLGGCLVEPNDVVLSPSIRGTKEDMQRYLDWEL
                     TLTK"
     gene            complement(705..1265)
                     /locus_tag="VF089_21945"
     CDS             complement(705..1265)
                     /locus_tag="VF089_21945"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX6176680.1"
                     /translation="MAYSLQDFIEDLDRITRTETSQKNIVTIAKPLLAKLVEQPGCID
                     EKYTRRGMTAYGRYMLHRAPLFNISSVVWGPGDNARAHNHDTWGLVGVIENEIQETRF
                     RRRDDGTEAGYADLEAIGVVRNQAGMVSCLVSPSDDIHEMNNVTDRNTVEIHVYGKDL
                     TDMPRLRFDIASKAVKTFASPKYDNC"
     gene            1710..2336
                     /locus_tag="VF089_21950"
     CDS             1710..2336
                     /locus_tag="VF089_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6176681.1"
                     /translation="MPTANLEDMQIYYEDVGEGEAVLLAPPSWWPCDTWKIKVVPFLS
                     RRYRTIVFDCRGTGHSSKPDHGYTISQFAKNCAGLLEHLKITRVHAAGFALGGQIAQA
                     LAIGRPDLISTLTIAAAGAGIMPNRDVPRETRNTDEEEIREHGFERFIRGHVENTHMA
                     FGAQFYKEHPEIVLALSDALWKRQQVRSSIAITTRRGAPRTSSATRRK"
     gene            2339..2542
                     /locus_tag="VF089_21955"
     CDS             2339..2542
                     /locus_tag="VF089_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176682.1"
                     /translation="MPTLILCGADDDVNRSGSTPVRTARRLAELVPGSELVLVPNVKH
                     MTFWDGNGALSALENFLQRHAMQ"
     gene            complement(2622..2951)
                     /locus_tag="VF089_21960"
     CDS             complement(2622..2951)
                     /locus_tag="VF089_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176683.1"
                     /translation="MKTENPSLYDEHACILNEKSQALMFDASYANDCLIITMPPPEAM
                     AQWDMNEDSRELKRIVRDLRWTGMISDALVVLAITGAVYFSPSLETMRAALEKTAISV
                     AEAPRAH"
     gene            complement(3900..4103)
                     /locus_tag="VF089_21965"
     CDS             complement(3900..4103)
                     /locus_tag="VF089_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6176684.1"
                     /translation="MKTLEKILERPSFWQLVFSHSKVNGAPYNGEQLPGRIWDWPKDT
                     YLQKRLHESKSKWYHSANRAELN"
BASE COUNT          928 a         1133 c         1220 g         1034 t
ORIGIN      
        1 atgtcccagg tgggcaggcg gtgcagcgcg cggatgattt cgttgcggtg cgcaccgggc
       61 aaatcgtctt catcagcaac gaatctgcgc gcgcggtcat ggcggcatat tggttccgac
      121 agatgggttt cgctcattca gcggtgctgc aaggcgggct gcgcgcatgg acgcagggcg
      181 gcgtagaaat tgagaaagga tcactccgca atgacccggt ttgtttggaa gctgcgcggc
      241 aggcggtgcg tgtcgttgat ccttcaacta tcgaaggtaa aacgcgtgat ccttcggttt
      301 tgcttctcga tgtcggcacg agtctcgact tcgaaacggc gcatgttccc ggggctaaat
      361 ggatttcccg cggctggctc gatctgagat tgccagaaac atatcccgac cgtaatcagc
      421 cgatagtcat gacttgtgcc gacggccgcc agtcgctact cgcggcgctc gcactcggcg
      481 agttggctta cagcgacgtt gtagttatac acggcggcgt gcgggcatgg tcggcggctg
      541 gattccctac tgaacaggga ctaggcggct gcttggtcga acctaacgat gtggttttgt
      601 cgccctcgat ccgcggcacc aaggaagata tgcagcgtta tctggattgg gagttgacac
      661 tgacgaagtg attgcagcaa agcggcgaac gcttatgccg cacctcagca gttgtcgtac
      721 ttgggactgg cgaacgtttt gacggctttg ctggcgatat cgaaacgcaa tcgcggcatg
      781 tcggtcagat ctttgccgta aacgtggatt tccaccgtgt tgcgatcggt gacgttattc
      841 atctcatgga tgtcgtcgct gggcgagacc aggcaagaaa ccattccagc ttgatttctt
      901 accacgccga ttgcttcaag atcggcgtag ccggcctccg tgccgtcgtc ccggcggcgg
      961 aatcgggttt cttgaatttc gttttcgatc acaccaacca ggccccaggt atcgtgatta
     1021 tgggctctgg cgttatcgcc gggaccccaa accactgagg agatgttgaa tagcggcgcg
     1081 cggtgcagca tgtagcggcc gtacgctgtc atgccgcggc gtgtgtactt ctcatcgatg
     1141 cagcccggct gctcaacgag cttggcgagc agaggcttcg ctatggttac gatgtttttc
     1201 tgcgaagttt ctgtgcgggt gatccgatcg agatcctcga taaaatcctg cagtgaatag
     1261 gccattctca ttttctagca cgaatcgcag gaaggtcaaa agtaagcacg gggttttcga
     1321 ccgcccgctg cggaattcgg ccacctagac attgatcaga tcatcgaggt gatttctcaa
     1381 cggttcgaag aactggcttc atccgaagca agagggaccc aggagtctaa gatggatcgg
     1441 caggggactt tccgattagg tttttgctgg gtttctttac taacgaaaaa cgcttcgcag
     1501 cctggtcttg cctgccgaag acgacgcctt atctacaaat gttgatcgct tcagtatccg
     1561 gagagccgga cgccaatccg gacttcaagc gcatcgcaat ccggtgagtg gcccatgcac
     1621 gtgatcgaca gcgaaaacgt tttggattac gtctcgaccg acagcaatca cgtacgggag
     1681 gggtctgaac ctatttgagg ggagatgaga tgccgacggc caatttggaa gatatgcaga
     1741 tctattatga ggatgtggga gaaggcgaag ctgtattgct cgcgccgcct tcctggtggc
     1801 cctgcgacac ttggaagatc aaagtcgtgc catttctgtc gcgccgttac cgcacgatcg
     1861 tttttgattg tcgcggcacc ggccatagca gcaaaccgga tcatggctac acaatcagcc
     1921 agttcgctaa aaactgcgcc gggcttctgg aacatctaaa gatcacccgg gttcatgcgg
     1981 cgggatttgc gctcggcggc cagattgctc aggctctcgc aatcggccga ccggacttga
     2041 tatcgacctt gacaattgcg gccgcaggcg cgggaatcat gccgaaccgc gacgtgccgc
     2101 gcgagacgcg caacacggat gaggaagaga ttcgcgagca cggcttcgag cgcttcatcc
     2161 gcggtcatgt cgagaacacg cacatggcat tcggtgcaca attctacaaa gagcatccgg
     2221 aaattgtgct ggcgctgtcg gatgcgctgt ggaaaagaca acaagtccgg agcagcatcg
     2281 ctatcacgac gaggcgcggc gcacctcgga catcctcggc aacgcgccga aagtaaaagt
     2341 gccaaccttg attctttgcg gagcggatga cgacgttaat cgcagcggca gcactccggt
     2401 gcgaacggca cgtcgacttg cggagcttgt tcccggcagc gagctggtgc tcgttccgaa
     2461 cgtcaagcat atgacgtttt gggacggcaa cggcgcgctg agtgcgttag agaatttctt
     2521 gcagcgacat gcgatgcaat aagctgatct gttccacgag caggcggacg atagtgtgct
     2581 ggaactgagg caatggtggt gggggctaaa gtagattgag cttagtgggc tcgcggcgcc
     2641 tcggctacgg aaatcgcggt tttttccaaa gcagcgcgca tagtttccag gctaggggaa
     2701 aaataaaccg ccccggtgat tgctaagaca accaacgcgt cgctgatcat gcctgtccag
     2761 cgaagatcac gtacgatacg tttcaattcg cgagagtctt cattcatgtc ccactgggcc
     2821 atcgcttcgg gcggcggcat cgtaataatc aaacaatcgt tggcgtagct tgcgtcgaac
     2881 atcaaggcct gcgacttctc attgaggata catgcgtgct catcatacag tgaaggattt
     2941 tctgttttca tcgcattccc ctcctaggag tcacgtccat tcattagcgc cactgtttgc
     3001 ggttggcggc ggccaatctg ttattcctgt tatgtccgcg ggtgacggtt taggtttcgt
     3061 tgcattctta ttcgcaatcc gtgcgcggtt ctaatagcaa cggttgtgcc gattgaagtc
     3121 tcatgtgagc gcatgatgcc gggtgtcaaa acaaagcttt atcaaggatt tgtagttcta
     3181 agactaagaa ggtccatccc ggaatctaac acttatcgtt cactcgatca ttttcgtacg
     3241 acacgtacat gctttcgccg aactcgtaaa tgagagttta ggctgaactc tccgtctcct
     3301 tcctggaaca attccatccg atggtggtga atcgagatta cctcgacgag cagctcaatc
     3361 tcatcccaga tctgcgtgag tgcacgatcg gcaaacgtat atcaccggat aattcaatcg
     3421 tccgctgctt cgatatgcag tttgcccgtg ctctgagcaa actgctatat gacagactta
     3481 ggctgtgccg ccatatgcga aactacgagt ctcatcgcct catcccgcgc tcggctctag
     3541 gcggccatgg cgctgtcgct gatcgaaatc gcgcaggcgt tgcgtgcggc aggcttcgcc
     3601 taactctttc aaggcacctc cggtaagact tacaaataag ctacccctgg atccgcttgg
     3661 tattcaaagg ctggcgtgac gccttcgcta ccgcctctcc tcttacttga gcgtaggctt
     3721 gcccggtcac tgacagttca caaacatcag acaaatctaa atcgatgatg gaaaatcgaa
     3781 cgcaaaaaaa atcggccagg ctttgggggg cctggccgat gccactgaac cgggtgagtc
     3841 cagtggtgcc gttcttcccg agtagctgtg ctgaaggagg cagatcgaga agcggcgaac
     3901 taatttagct cagctctgtt cgcactgtgg taccatttcg attttgattc atgaaggcgc
     3961 ttttgcagat acgtatcttt cggccaatcc caaatccggc caggcagctg ctctccatta
     4021 tacggcgcgc cgttgacctt actgtgagaa aaaaccagct gccaaaaact cggtcgctct
     4081 aaaatttttt ccagggtttt cattttattc tctccttgaa tgatattttg gtttgctctg
     4141 ctttttgatt gcgtcctggc tccggaacag cagacggcgt gccgggcctg cgacgtccac
     4201 tgtaagccaa agttgtcgga ttttttgccg agctactccc tggatagctc cggcgagtgc
     4261 gacccctttg cgggttgcgg aggattatcg gtcagaattg ccggctgcta tttcg
//
LOCUS       DASUTG010000941         6377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1183138, whole genome shotgun sequence.
ACCESSION   DASUTG010000941 DASUTG010000000
VERSION     DASUTG010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183138"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VF089_21970"
     CDS             complement(<1..1122)
                     /locus_tag="VF089_21970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6176685.1"
                     /translation="MSEVRRGPLTGVRVLDLTRVVAGPYCSMFLGDLGAEVVKVEQPG
                     AGDDTRGWGPPFAGGESAYYLCINRNKQSLALDLKSPPAVELLRELVKVADVIIENFR
                     PGTMERLGLGEKELRELNPRLIFASLTGFGADGPMSDWPGYDLIVQAWGGLMSITGAP
                     DGEPVKVGVAIIDLVAGLMLGKAITAALFAREKIGVGQRIDTSLLEAEVASLINVGSN
                     YLIGGKVPTRWGNAHPNIVPYQNFQTADGYLVIGVASEVIWKRFCEAIGRPELAEDAR
                     FADNSKRVENRSELIALLSETFSQQRNEAWFKLLTAAEVPCAPVQTIDQVFQAPQVLQ
                     RDMLIECDHPTAGKVRMAGIPVKFSVTPATIRMPPPLLGE"
     gene            complement(1119..1493)
                     /locus_tag="VF089_21975"
     CDS             complement(1119..1493)
                     /locus_tag="VF089_21975"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6176686.1"
                     /translation="MAEDPKEAFKKVGLGSANNAYDIIAKLDPEYFERLKGLYVDGTF
                     GRDGALTRKTKELIMIGICCALLRPRGIRLHSERALTLGASPKEVMEAMEVAAIPGGM
                     PGLWAGVETLHEILQAKGIEFK"
     gene            complement(1627..3318)
                     /locus_tag="VF089_21980"
     CDS             complement(1627..3318)
                     /locus_tag="VF089_21980"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6176687.1"
                     /translation="MAANGRQKTVAQAYLEVAVDHGVDYVFGLPGTSGQEFIGTIADQ
                     ERVRFVLALHETCVVSMADGHARVTGRPSLAQVSTLPGSVNAVGALYDAYRDRSPVVV
                     TSTHVDTRIVGRDSHTEGKDLVELTRQFTKWSAEVHRSDRIPEFLNRAFKVAATPPTG
                     PVYLSLPSNLLGENIDQPNPVAERSRITPRIAADPEAVQQAAQMLANAKRPLIVAGSG
                     IAKSGGTDELIKFAEMLGAPVVMEPRYSFLSFPTDHPQSFQIAERNPSFNLPVWGEPD
                     VIVAIGCRLIREYRYLPEPVMKPDTRCIHIEEDPWEIGKVFPVDLGIIADGRSALRSL
                     LEVFPKVAAAANGREERLAVIRKAQEQVKAELEARVKQGWDGTPINAARLARTMDRLL
                     EGDTLIVNESPTSKDILMSNFNFSTGRSYFSNSSGGYLGWGLGAAIGAKLAAPQRRVV
                     ACLGDGSSMFGLQGLWTLAKYRVPLIVIVFNNRAYMAVKNQFRGSEERIRLAAEMGAE
                     LVGPELNFARLADTFGIFGQRVEQPDAIEPAIKRALEQNGPALIDVVIGQNTRKD"
     gene            complement(3456..4451)
                     /locus_tag="VF089_21985"
     CDS             complement(3456..4451)
                     /locus_tag="VF089_21985"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6176688.1"
                     /translation="MKLIILIGLLTLCMFSQAGDAFSQDNLERMHIGYSAQAGAFAPI
                     WITKEAGLFKKNGLDVNLLFIPGGPTAAASMLAGEVQAVAMAGPAVVSSNLGGTDLVM
                     IAGIVNTFAFQVITVKGITSPSQLKGKRLGVNRFGTAPDIAARFALRRMGIDPSEVTI
                     LQLGEQSTRLMAMKAGQLEAAIVLPPITTIAQREGMNVLLDMSELGAEYQITGLASSQ
                     SFINKNRSSAMKLMRSFVEGIHFYKTRKQESMAIIAKYMRTNDMEAVGATYDYFANKI
                     VPKKPYPTAHGIKALLELAAKDRPEAAKVPPERIMNTSLLKELDDSGFIDRLYRN"
     gene            complement(4533..5375)
                     /locus_tag="VF089_21990"
     CDS             complement(4533..5375)
                     /locus_tag="VF089_21990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176689.1"
                     /translation="MRAIDVHVHPSTRGLDTHACSYFRRELSEVPQTADGFADVYLQQ
                     NVKAFLIGWHPSTVTEGARNSNEHAIELVSNYPKSFCGVLASLDTNSTDLPGVARYAE
                     ELVRDPKVIGFKFHPPDQGFYPSDKRYYDIWEVLQAGGKPAMFHIGFTVLGANTPGGS
                     GIGLDYGRPIHLDTLAKDFPRMKILAAHPGWPWVEETVGVVTHKKNVYVDTSGYIAEQ
                     LPELFQKAIGTRLQDKALFGTDFPYIDLAKALTSFDRMNFKDSVKEKLLLANARNLFG
                     LAAE"
     gene            complement(5362..>6377)
                     /locus_tag="VF089_21995"
     CDS             complement(5362..>6377)
                     /locus_tag="VF089_21995"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX6176690.1"
                     /translation="NEIEKVKKHLVTARLSDMTSASLVTEAVSESGNIKRSVYRTLRE
                     TGFSGVLTTNTSSLTRKSLIESGDYPYEKFSTTHFFNPVLYSQMVEVVRGDINRSNYA
                     TLMSFLSDLGRKPIETKDISGFVSNSVLMVYALMALRLLEAGATIEAVDGAAKELRSL
                     PPLFSFDSWKPSIVEDVTRVMWEFRGDDFLRSSTLLAALAKSNPLFYAGQKPNEAIYD
                     LIEKRGSGLDPSMLKLALLTSMRVAAVRTVELGESPATVDFISTEGLKFPRAPLAEID
                     DLGAEAVLDDLARVNEAIWEGKMQAPELLTAMAKKRHSFFKDGKPNPAIEPLIRGSAH
                     ARH"
BASE COUNT         1359 a         1880 c         1714 g         1424 t
ORIGIN      
        1 ttcgccgagc agaggaggcg gcatgcggat ggtggcgggc gtcactgaaa acttcaccgg
       61 gattccggcc atgcgaacct tgcccgccgt cggatgatcg cattcgatta gcatgtcacg
      121 ctgtaagacc tggggcgctt ggaaaacctg atcgatggtc tgcaccggtg cacagggaac
      181 ttccgcagcg gttaacaact tgaaccaagc ttcatttcgc tgctgtgaaa atgtctccga
      241 gagcagtgcg atcaactcgc tgcgattctc gactcgcttt gaattgtcgg caaaccgggc
      301 gtcctccgcc agttcagggc gcccgatggc ctcgcagaaa cgcttccaaa tcacttcgct
      361 agccaccccg atcaccagat acccatccgc ggtttggaag ttttgatacg gcacgatgtt
      421 cggatgagcg ttgccccagc gcgtaggtac tttaccgcca ataagataat tgctgccaac
      481 attgatcagc gaggccactt cggcttcgag caaagaagtg tcaatccgct ggccgacacc
      541 gattttctcc cgagcgaata acgccgctgt gatcgctttg ccgagcatca gcccggcgac
      601 cagatcgata atcgccacgc cgactttgac cggctccccg tccggggcgc cagtgatgct
      661 catcagaccg ccccatgctt gcacgatcaa atcgtaaccc ggccagtcgc tcatcggtcc
      721 gtcggcgcca aagccggtga gagatgcaaa gatgagccgc gggttgagct cgcgcaactc
      781 tttttcgccc aagcccagcc gctccatggt tccagggcgg aagttttcga taatcacgtc
      841 ggcgacctta accaactcgc gcagcagctc gaccgctggt ggtgatttta gatcaagggc
      901 caggctttgc ttattgcggt tgatacacag gtaataagcg ctttccccgc ccgcaaacgg
      961 tgggccccac ccccgtgtgt cgtcacccgc tccgggctgt tccaccttga cgacttcagc
     1021 gccgagatca ccaagaaaca tcgagcaata gggtccggcg acgactcgcg taagatcgag
     1081 aacgcgcacg ccggtaagtg gcccccgccg aacttcgctc acttaaactc gatgcctttc
     1141 gcctggagaa tttcgtggag agtctcgacc cctgcccaaa gcccgggcat gcctccgggt
     1201 atggccgcga cttccatcgc ttccatcact tctttcggcg aggcgccgag ggtgagcgcg
     1261 cgctcgctat gcagccggat cccgcgcggg cgtagcaacg cacagcagat gccgatcata
     1321 atcagctcct tggtctttct cgtcaacgcg ccgtcgcgac cgaaggtacc gtcgacgtaa
     1381 agtcccttga gcctttcgaa gtattcaggg tccagtttgg caatgatgtc gtaggcgttg
     1441 tttgcggaac ccagacctac ttttttgaag gcttctttcg ggtcttctgc catatttcgt
     1501 ccccctcttg ttcgcccgtt cgtgtaggat gaggcctgac caccacgaag aacacgaagg
     1561 acacgaagaa ttaagaaaac agatgcaacc ttcgtgctct tcgtgacctt cgtggttcac
     1621 cgatcctcag tccttgcgcg tattctgacc gatgacgacg tcgatcagcg ccggcccatt
     1681 ctgctccagc gcccgcttga tcgcaggttc gatagcgtcc ggctgctcga ctctttgccc
     1741 gaagataccg aacgtgtcgg cgagccgcgc gaaattcaac tcaggcccga ccagctcggc
     1801 gcccatctcg gcggcaagac ggattctttc ttccgagccg cggaactgat tcttcaccgc
     1861 catgtaggca cgattgttaa acacgattac tatcagcggc acgcgatatt tggccaaggt
     1921 ccagagtccc tggagcccaa acatgctgct gccgtcgccc agacaggcaa ccacccggcg
     1981 ctgcggcgcg gcaagcttgg cgccgatggc tgcgcccagg ccccagccga gatacccgcc
     2041 ggacgagttg gaaaaatagg agcggcccgt tgagaaatta aaattagaca tgagaatatc
     2101 tttgctcgtc gggctttcgt taacgatcaa ggtgtctcct tcgagcagac gatccatggt
     2161 ccgagcaagc cgagccgcat tgatcggcgt gccgtcccag ccctgcttca cgcgcgcttc
     2221 gagctcggct ttcacctgtt cttgagcttt gcgaatgacg gccaggcgtt cctcgcggcc
     2281 attcgctgct gcggccactt tcggaaagac ctcgagaaga gaacgcagcg cactcctgcc
     2341 atcggcgatg atgccaagat ccactggaaa aactttgccg atctcccacg gatcttcttc
     2401 gatgtgaatg cagcgagtgt ccggcttcat caccggctca ggcagataac ggtactcgcg
     2461 aatcaaacga cagccaattg cgacaatcac gtccggttca ccccatacgg gcaggttgaa
     2521 agaagggttc cgttcggcaa tttgaaagct ctgcggatga tcggtcggga aactcaagaa
     2581 agaataacgc ggctccatca ctaccggcgc cccaagcatc tcggcgaact tgatcaattc
     2641 atcggtgccg cccgatttcg cgatgccgct gccggcgacg atgagcggtc gcttggcgtt
     2701 ggccagcatt tgcgccgctt gttgcacggc ttcgggatcg gcggcgatcc gtggcgtgat
     2761 gcgcgaccgt tccgccacag gatttggctg atctatgttc tcgcccagta gattgctcgg
     2821 aagcgataga tacaccggcc cggtgggcgg cgttgccgcg actttaaatg cgcggttgag
     2881 aaattccggg atgcgatcgg agcgatgcac ttccgcgctc cacttagtga actgcctggt
     2941 caattcgaca agatctttcc cttcggtgtg ggaatcgcga ccaacgatcc gagtatcgac
     3001 atgggtcgag gttactacca ccggagagcg gtcgcggtaa gcgtcgtaga gagcgccgac
     3061 ggcattgact gaaccgggaa gggtgctcac ctgcgcgagt gacggccggc cggtgacccg
     3121 agcgtggcca tcggccatcg aaacaacgca ggtctcatgg agcgcaagca cgaatcggac
     3181 cctttcctgg tcggcgatgg tgccgatgaa ctcctgcccg ctcgttcctg gcagaccaaa
     3241 aacataatcg acgccatggt cgaccgcgac ctcgagatag gcctgcgcca cagttttctg
     3301 cctgccgttt gcagccatat tctttctcct ctataagtgc gttcagcgtc tcgttagatc
     3361 atttaattgt tgaaggtcaa aaccgcgcga gttccaatcg ttccagtatt tcaacgagct
     3421 ctaaatgaaa aagaccattg aaccttgaat taagctcaat ttcgataaag ccgatcgata
     3481 aacccgctgt catctagttc cttcaacaac gacgtgttca taatgcgttc tggcggtacc
     3541 ttcgcggctt cgggacgatc tttcgccgcg agctcgagta aagccttgat tccatgagcg
     3601 gtcggatacg gcttcttggg aacgatctta ttggcgaagt aatcataggt cgcgccgacc
     3661 gcttccatgt cgttggtgcg catgtatttg gcgatgatcg ccatgctttc ctgcttccga
     3721 gttttgtaaa aatggatccc ttcgacgaag gaacgcatga gcttcatcgc ggaagagcga
     3781 ttcttgttga tgaagctttg gctgctggcc aagccggtga tctgatattc cgcgccgagt
     3841 tcggacatat ccaagagcac gttcatgcct tcgcgttgcg cgatagtcgt gatcggcggc
     3901 agcacgatgg cggcttcgag ttgtcccgct ttcatcgcca tcaggcgggt cgactgttca
     3961 ccaagttgca ggatcgtcac ttcgctcgga tcgataccca tgcgccgcaa cgcgaaacgc
     4021 gcggctatat ccggcgccgt gccgaatcga ttgacgccca agcgcttccc cttgagctgg
     4081 ctcggcgatg taattccctt aactgtgata acctgaaacg caaatgtgtt gacgatgccg
     4141 gcgatcatca cgagatcggt gccgcccaga ttgctgctta cgaccgccgg accagccatt
     4201 gctaccgcct ggacctctcc tgcaagcatc gacgccgccg ctgtcggccc gccgggaata
     4261 aaaagcagat tgacatccag cccgtttttc ttgaacaagc cagcctcttt tgtgatccag
     4321 atcggcgcga atgcccccgc ctgggcgctg taaccgatgt gcattctctc aagattgtct
     4381 tgggaaaacg cgtcacctgc ctgcgagaac atgcacaggg ttaaaaggcc gatcagtatg
     4441 ataagtttca aatatcacgc tccgattctg cttccgcaat tactgatgtg aacaccatcg
     4501 cggctgtggc tcgttgatat tcgtcgccgg agctactcag ccgccagccc gaacagattc
     4561 cgcgcgttgg ccagaagcag cttttccttg acggaatctt tgaaattcat cctatcgaaa
     4621 cttgtaagcg ccttggctag gtcgatgtag gggaaatccg tgccgaaaag cgccttgtcc
     4681 tgcagccgtg tgccgatcgc cttctggaac aattccggta actgctctgc aatgtaacca
     4741 gaagtgtcga cgtagacgtt tttcttgtgt gtcaccacac cgactgtctc ttcgacccat
     4801 ggccaacccg ggtgtgcggc tagaatcttc atgcgcggaa aatctttggc cagtgtgtct
     4861 agatggattg ggcgtccata atcgagaccg atgccgctgc cacccggcgt gttcgcgccg
     4921 agcacagtga aaccgatgtg gaacatcgcc ggcttgccac ccgcctgcaa aacttcccag
     4981 atgtcgtagt acctcttatc gctcggatag aagccctgat cgggcggatg aaatttaaag
     5041 ccgatgacct ttggatctcg caccagctct tcggcataac gggcaactcc tggcagatcc
     5101 gtcgaattcg tatccagcga agccagcacg ccacaaaatg atttcggata attggatacg
     5161 agctcgatgg catgctcgtt ggaattacgc gcgccctcag tcaccgtcga aggatgccac
     5221 ccgatcaaga aggctttgac gttctgctgc aaatagacat cggcgaatcc gtcggccgtt
     5281 tgcggcactt cgctcaattc acggcgaaag tagctgcaag catgggtgtc gagcccgcgc
     5341 gtgctaggat gaacgtgaac atcaatggcg cgcatgagcg ctccctctga tcaaaggctc
     5401 gatcgctgga tttggcttgc cgtctttaaa gaagctatgc cgcttcttgg ccatcgccgt
     5461 caaaagctcc ggcgcctgca tcttgccttc ccagatcgcc tcatttactc ttgccaaatc
     5521 gtccaagacc gcctccgccc cgaggtcatc gatttcagcg agaggcgcgc gtggaaattt
     5581 caagccctcg gtagaaatga aatcgactgt tgccggcgat tcacccaact cgactgttct
     5641 tacggcggca acccgcatcg acgtcagaag agccagcttg agcatcgaag gatcgagccc
     5701 gctgccgcgt ttttcgatca ggtcatagat tgcctcattg ggcttctgac cggcataaaa
     5761 cagcggattg cttttggcca atgcagcaag cagtgtggaa gatcgcaaaa aatcatcgcc
     5821 gcggaattcc cacatgacgc gcgtgacatc ttcaacaatg gaaggcttcc agctatcgaa
     5881 actgaacaaa ggcggcaatg accgcagttc tttggctgca ccatcgacgg cctcgatggt
     5941 cgcaccggcc tccagcaaac gaagcgccat caacgcgtaa accatcaaga cgctgttcga
     6001 tacgaatccc gaaatgtctt tcgtctcgat cggtttccgg ccgagatcac tgaggaacga
     6061 catcaaagta gcgtagttcg atcggtttat gtctcctctg acgacctcga ccatttggct
     6121 gtacagcacc gggttgaaaa agtgcgtcgt ggaaaatttc tcgtaagggt agtcgccgct
     6181 ctcgatcagg cttttccggg tcagtgacga tgtattggtg gtgagaactc cactgaagcc
     6241 ggtttcgcgc agtgtccgat acacggatct cttgatgttg ccggattccg atacggcctc
     6301 agtcaccaga gaggcgctcg tcatatcgga cagcctcgcc gtgactagat gtttcttgac
     6361 cttttcgatt tcattct
//
LOCUS       DASUTG010000942         3508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1628731, whole genome shotgun sequence.
ACCESSION   DASUTG010000942 DASUTG010000000
VERSION     DASUTG010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628731"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF089_22000"
     CDS             <1..802
                     /locus_tag="VF089_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HEX6176691.1"
                     /translation="KLLQAAYKSGYGAQFGGKYFALDVRIIRLPRHGASCPVGMGVSC
                     SADRNVKAKINRDGIWLEELERDPGRLIPEEYRGKVTSNVVRVDLNRPMKEILAQLSK
                     HNVTTQLSLTGTMIVARDIAHAKIKERLDRGEGLPQYLKDHPVYYAGPAKTPEGMPSG
                     SFGPTTAGRMDSYVDLFQSHGGSMIMLAKGNRSQQVTDACKKYGGFYLGSIGGPAAIL
                     AKENIKKVEVLEYPELGMEAVWKIEVEDFPAFILVDDKGNDFFKQILH"
     gene            complement(825..1907)
                     /locus_tag="VF089_22005"
     CDS             complement(825..1907)
                     /locus_tag="VF089_22005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176692.1"
                     /translation="MRIIDADGHVLERDIPWADLLEEPYRSRAPRAVKDNRGFNFVMI
                     DGKLTPKPVGKACSFVGAPRSHHPQPTTGMVDPRQRLKDMDLEGIDTAVLFGTSPFLS
                     LPFVEDKDLACAVARVYNNWLADYCKADPRRLKGVALTAIQDPIEAVRELRRAVEELK
                     FVAVATPPISASGKNLDHPDLYPFFAEAQRLEVPICIHVGAGDGVPAGTERFDHPLYT
                     HALAHPFEQMIAALCVVAGGLLEKFPRLKFAFMEAGCGWVPYWMERLDEHYEILQPTV
                     PWLTKPPSEYMRSGQMFYAFEMDEKMLPYVAEFVGADRLVFATDYNHSDSKFPHTVEE
                     VMERSDISQGLKAKIMGENAAKLYNL"
     gene            complement(2248..3423)
                     /locus_tag="VF089_22010"
     CDS             complement(2248..3423)
                     /locus_tag="VF089_22010"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6176693.1"
                     /translation="MSKLNEKIVTFLDPQAIEPAAKQQLLNMAELPFVFKHIAVMPDC
                     HLGKGATVGSVIATKGAVIPAAVGVDIGCGMIAVKTKFFAKDLPDSLEKTRTGIERRI
                     PLGAGAFNKKLTTTAHKRVAQLKALGRDYQAVDRRWTEALGSLGSGNHFIEISLDESE
                     QLWVVLHSGSRGIGNKLAMKHIKTAQKLMDQDQVHLKDRDLAYLVEGRKEFSDYITDL
                     LWAQDFALLNREEMMDRVMTELSYLFYKEDGHQAEIELERINCHHNFTQRENHFNENV
                     WVTRKGAIQMKQGQKGVIPGSMGTRSYVVSGLENQMAYHSAPHGAGRRFSRGEARRRF
                     TMDDFRKAMAGIECRHSAKLIDELPMAYKDIDEVMENSRDLVKVEHTLRQLVNVKGD"
BASE COUNT          775 a          941 c          924 g          868 t
ORIGIN      
        1 gaagctgttg caggcggcgt acaagtcggg ttacggggcg caatttggtg gcaagtattt
       61 cgcgctggac gtgcggatta tccgtttgcc tcgccacggc gcgtcctgtc cggtcggtat
      121 gggcgtgtcc tgttccgcag atcgcaatgt caaagccaag atcaaccggg atggaatctg
      181 gctcgaagaa ttggaacgag atccaggccg actgattccg gaagaatatc gcggcaaggt
      241 taccagcaat gtggtgcgag tcgatctcaa ccgtccgatg aaggaaatct tggcgcagct
      301 ctcgaagcat aacgtaacaa cgcagctttc acttaccggc acaatgatcg tggctcggga
      361 tatcgctcac gctaaaatca aagaacgttt ggacagaggc gaaggattgc cgcagtatct
      421 caaggatcat cccgtatact atgccggtcc ggccaagact ccagaaggga tgccgtccgg
      481 ctctttcggt ccgaccacag cggggcggat ggattcctat gtcgacttgt ttcaatccca
      541 cggcggatcg atgatcatgc tcgccaaggg taaccgcagc cagcaggtta ccgatgcgtg
      601 caaaaagtac ggcggctttt atctgggatc catcggcggg ccggcggcga tactcgcaaa
      661 agaaaacatc aaaaaagtcg aagtgctgga atatccggag ctcggaatgg aagcggtttg
      721 gaagatcgaa gtcgaagact tcccggcgtt tatcctagtc gacgacaagg gcaacgattt
      781 cttcaaacag attctgcact gagataagtt ggagactcgc gggcttacaa attgtagagc
      841 ttggcggcgt tctctcccat gatctttgcc ttcaatcctt gcgaaatatc gctgcgctcc
      901 atgacttctt ctaccgtgtg cggaaacttg gaatcgctat gattatagtc ggtggcaaac
      961 actagccgat cggcgccaac gaattccgcc acgtagggta gcattttctc gtccatctca
     1021 aaggcataga acatctggcc gctgcgcatg tattcgctcg gcggtttggt gagccaaggc
     1081 acggtcggct gcaggatctc ataatgctca tcgagccgct ccatccagta cggcacccag
     1141 ccgcaacccg cttccataaa ggcaaacttc agtctgggaa atttttcgag caagccgccg
     1201 gcgacgacgc acagcgcagc aatcatctgt tcgaacgggt gcgccaaagc atgagtgtac
     1261 agcggatggt cgaatcgttc ggtgcccgcg ggcacgccgt ctccagcgcc gacgtgaata
     1321 caaatcggca cttcgagtcg ctgcgcttcg gcaaagaacg ggtagagatc gggatgatcc
     1381 aaattctttc ctgatgccga aatcggcggt gtcgccaccg ctacgaattt cagttcctcc
     1441 acggcccgac gtaattctct taccgcttcg atcggatcct ggatcgccgt aagagcaacc
     1501 ccctttaggc gtcgtggatc ggccttgcaa taatccgcca gccaattgtt gtaaacccgc
     1561 gctacggcgc aggcgagatc tttgtcctcg acgaaaggaa ggctcaaaaa aggagaagtg
     1621 ccgaagagca cggctgtatc gatgccttcc agatccatgt ctttcaggcg ctgtctggga
     1681 tcgaccatcc cggtcgtagg ctgtggatga tgactgcgcg gcgcgccgac aaaactgcag
     1741 gctttgccaa cgggcttggg tgtgagcttg ccgtcgatca tgacgaagtt gaaaccgcgg
     1801 ttatctttca ccgccctcgg agcgcgtgag cgatagggtt cttcgagcaa atcagcccac
     1861 ggaatatctc tttccagcac gtgcccgtcg gcgtcgatga tgcgcatcct aattgcctcc
     1921 tgggcagact gctgaaaaaa ctcacatgct tcgttgcgct caaatcgcct cgcctcaacg
     1981 tactttgcgc gtacgcctct gctcgtcgat tttcgtgtgc ctcgcctttg agattttgag
     2041 cagctgcaaa cactgttttt aacacactac tctggctgtc gtcttgtagg gatatcagaa
     2101 caatttggtt ttgctcaaga gacgacgccc atcgcgaatc tagcaatgtt tccaaacttg
     2161 gaaaccgtac gtggtagttt ttttgatctt ccgccgagct gtttctatga ggtatgaacc
     2221 gtaggcgagt tttgtcgttc tttgcggtta atcacctttt acgtttacca gctgtcttaa
     2281 tgtgtgctcg actttaacca gatcgcggga gttttccatg acttcatcaa tatctttgta
     2341 agccatcgga agttcgtcga tcagctttgc ggaatgacga cattcgattc cggccatggc
     2401 tttcctgaaa tcgtccattg tgaatcgtcg ccgggcttcg ccacgtgaaa acctgcggcc
     2461 ggcaccatga ggtgctgaat gataggccat ctgattttcc aatcctgaga cgacatagga
     2521 acgcgttccc atcgagccgg gaataacccc tttctgcccc tgtttcattt gaattgcgcc
     2581 ttttctggtt acccatacgt tttcgttgaa gtgattttcc cgctgggtga agttgtgatg
     2641 acagttaatc cgctcaagct cgatctctgc ctggtggccg tcctccttat agaacagata
     2701 ggaaagctcg gtcatcaccc ggtccatcat ttcctcacgg ttcaacagag cgaaatcctg
     2761 tgcccagaga agatcggtga tgtagtcgct gaactctttt cgcccttcga caagatatgc
     2821 cagatctcga tccttcaggt gcacttgatc ttggtccatg agcttctggg ctgttttgat
     2881 atgcttcatc gccagcttgt tgccgatgcc gcgcgagccg gagtgaagga ctacccaaag
     2941 ttgctctgac tcatcgagag agatttcaat gaaatgattg cctgaaccca aagagccaag
     3001 ggcctcggtc cagcgccgat ccacagcttg ataatctctt cccaacgctt taagttgcgc
     3061 tactcttttg tgggcggtgg tggtgagctt tttgttaaac gcccctgcgc ccaaaggtat
     3121 tcgtcgttcg ataccggtcc gtgtcttctc aaggctgtcc gggagatctt tggcgaaaaa
     3181 ctttgtcttg acggcaatca ttccacagcc aatatcgact cctaccgcgg ccggaattac
     3241 cgctcccttg gtggcgatga ctgaacccac ggttgcgcct ttgccaagat ggcaatccgg
     3301 cataaccgct atgtgcttaa acacgaaagg cagctccgcc atattgagga gctgctgttt
     3361 ggctgcaggt tcaatcgcct gtggatctag gaaggtgacg attttttcat tcagcttgct
     3421 catgatttca ctatagcaaa ctgctcggca gtccgcgtgg gggataagct tgtcccgtag
     3481 ccgaaccacc actcaaagga gtctggtc
//
LOCUS       DASUTG010000943         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_2075293, whole genome shotgun sequence.
ACCESSION   DASUTG010000943 DASUTG010000000
VERSION     DASUTG010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075293"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1351..>2385)
                     /locus_tag="VF089_22015"
     CDS             complement(1351..>2385)
                     /locus_tag="VF089_22015"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6176694.1"
                     /translation="MDGKELLQKHVVVEGHRDVYEQLYRTSIGEPMPIRDAIAPRLIR
                     DGINLSVYAISGDSYSHSQNTGRYLETALENIDQFLQEAPKSEGMISMVRTRGDLPDK
                     IEPGTIKFLLHFEGCMPLRGSIQNLRNFYRLGLRSLQPVWNFRNELGDGVWENRTGGG
                     LTNFGVEVIKEANRLGMIVDLSHMNREGFFQSLEVATAPLIVSHANACGMLDSVRNLA
                     DDQIKAIADQGGVVGILALPERVGQGEVGIEDMLKHMDYMINLVGVEHLALGMDFVKY
                     DGPRTLKDRHHPLHKDPLIKGFEEIEDLPNLIDGLQNHGYHENEIALILGGNYLRVLK
                     TILPEQSVIY"
BASE COUNT          620 a          616 c          597 g          552 t
ORIGIN      
        1 catcaaagag aagtgagcaa tgcagatttc gaagccctat acaaatcgcc agctccaaaa
       61 ctaattcggt ctacatcgaa acatcctggg ggaaagtgtt tcggctacat tccgactgcg
      121 aaaatggcag gaacggtgaa caggtgtacg cactcatata gctcgcagca ttgtcgggct
      181 ttaaagttat tctaaatgtt cttgttcgag taaaggcaat cgctatctcc gcgtgacggt
      241 gccaatcgcc gcttcatatg tcttccctca acactcgtca atttccctcg cgtatttcca
      301 cagtctcata ttgctctcct cgtgtgtgga gtgtcgcggg gaagagagag caaatactca
      361 cccctctttg ttctgcgctc tgtgaccgaa tgtggaataa tggacaaggt gagaatctgg
      421 aaatctaaga gactgggcta ttaatgcacc ggcgtattat tcggcacaga ccaaaagcgc
      481 acagaggacg tgctgcagca ggaaggaaaa tcgccggact acttcgtcta attcgtacgg
      541 cgaaaccagt gcggactgag ccaactgccc tccgattaaa tccaatcgat tttgggaaag
      601 ataaacatgg gcggatacta gcccgagtaa atgtaaaatc gcaagtcgga atccgaaagg
      661 atgagctaat ttaacattgc gtttatagtc cgaagtcggg ggaactcggt caacactgtt
      721 agatcgaatt gtgagataaa gcaaaaggtc gcgccttcat ccgagttgtt tccacccaga
      781 gacggccgcc atgcgcctcg gtgatggatt tcgagaagca ccaaaaaaca caagaacgaa
      841 aaacttcgat tgacagctga tagttattca acctctcatc ggactgaatt cacgcccgaa
      901 ccctgtccgt atccgtcggt gcaatttact gatcctctag caaatctcgc tacagcgctg
      961 ttggactaac gtccaatagc caggtttcaa actctacgtt acaagaatct aagcccgaag
     1021 cgattttcag atgttgggag gagaagcacc tcgaggcatg agccagccat ggaatttgtg
     1081 tgccaacact gcgagcagtt gacaaagggg ccggcctata aagttaaaag tgaagaagcg
     1141 ggcgtgatct tacttgagat gatcgtatgt cattcatgtt ctgaacaagc aaaaaatctc
     1201 aggttagatg taaagagcat tacaagtgcg gagattaaat agactgagcc gaccgcaagc
     1261 aatggcctgc tcgcaagtga cgaaactcca ttccgcggat gaacggatac ccaaccaaga
     1321 gcaaaactcc gactctttag ttcccgcgac ttagtaaata accgactgtt cgggaagaat
     1381 cgtcttcaag acccgcagat agttgccgcc gagaatcaga gcaatttcgt tttcatggta
     1441 cccgtgattc tgcaggccgt cgataagatt gggcaagtct tcaatctctt cgaatccctt
     1501 gatcaacggg tctttgtgca acggatgatg acgatccttg agcgtgcgcg gcccgtcata
     1561 cttaacgaaa tccatgccca gagccagatg ctcgacgcct accagattga tcatgtaatc
     1621 catgtgcttc agcatatcct cgataccgac ttcgccttgg ccgacccgtt ccggcaacgc
     1681 cagaatgcct accacgccgc cctgatcggc aatcgctttg atttgatcat cggcgagatt
     1741 tcgcacgctg tcgagcatgc cgcaggcgtt ggcatggctg acgatgagag gtgcggtggc
     1801 gacttcgagg ctctgaaaaa aaccttcccg gttcatgtgc gacagatcga cgatcatgcc
     1861 cagccgattg gcctctttga tcacctcgac gccgaagttc gtgagccctc cgccggttcg
     1921 attctcccag acgccgtcgc cgagctcgtt gcgaaaattc caaaccggct gcaaggagcg
     1981 cagccctaat cgatagaagt tcctgagatt ctgaatgctg ccgcgcaaag gcatgcagcc
     2041 ttcgaagtgg agcaaaaatt ttatcgtgcc cggctcgatc ttgtccggca gatcgccgcg
     2101 ggttctcacc attgagatca tgccctcgga tttgggcgcc tcctgcagaa actgatcgat
     2161 gttttcgagc gcggtctcca ggtaacggcc ggtattttgc gaatgagaat aagaatcgcc
     2221 actgatagcg taaacggaga gattgatgcc gtcgcgaatc aatcgaggcg caatcgcatc
     2281 gcggatcggc atcggttcgc cgatggaggt cctgtagagc tgctcataaa cgtcccgatg
     2341 tccttcgacc acgacgtgct tttgcagaag ctctttcccg tccat
//
LOCUS       DASUTG010000944         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1332855, whole genome shotgun sequence.
ACCESSION   DASUTG010000944 DASUTG010000000
VERSION     DASUTG010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332855"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1097)
                     /locus_tag="VF089_22020"
     CDS             complement(66..1097)
                     /locus_tag="VF089_22020"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6176695.1"
                     /translation="MDGKELLQSHVVVEGHRDVYEQLYRTSIGEPMPIRDAIAPRLIR
                     DGINLSVYAISGDSYSHSQNTGRYLETALENIDQFLQEAPKSEGMISMVRTRGDLPEK
                     VEPGTIKFLLHFEGCMPLRGSIQNLRNFYRLGLRSLQPVWNFRNELGDGVWENRTGGG
                     LTNFGVEVIKEANRLGMIVDLSHMNREGFFQSVEVATAPLIVSHANACGMLDSVRNLA
                     DDQIKAIAEQGGLVGILALPERVGQGEVGVEDMLKHMDYMINLVGVEHLALGMDFVKY
                     DGPRTLKDRHHPLHKDPLIKGFEEIEDLPNLIDGLQKHGYHENEIALILGGNYLRVLK
                     TILPEQSII"
     gene            1173..2777
                     /locus_tag="VF089_22025"
     CDS             1173..2777
                     /locus_tag="VF089_22025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6176696.1"
                     /translation="MNFVIRKPRLLSSAVTLALLLIFVSCKTAPDSDSSASANHFRVN
                     LGTEPPSLDWSLATDGVSFNVIANLMVGLTEFTRDLKPAPVIAKSWDIADGGKRIIFH
                     LRDDVLWSDGQKVRARDFEYSWKRLLNPKTASEYAYILFDLVNAQEYHNGQLADAAEV
                     GVRAQDDVTLEVRLRHPAPYFLSLTTFEVTFPQRQDIVERFDSRWTDPEHIVTNGPFT
                     LASWKHENEIQLRANPNYFLGKPVIDRVTMLMVSEKTTALAMYEQGQLDFIDNRSIPT
                     LEKKRIAKQRGFHSVAQLRGYYYGFATHRKPFNDPRVRKAFAMAIDRDNFPKILQGGE
                     KPASSWIPPGMLAHNTKIGVHFNPPEARRLLREAGYPDGKGFPQVVLAYNTEEDHKLV
                     AEAVQSMWQRNLGVIVNLDNQEWKVYLKNLQHDPPHIFRLGWGADYPDPDNFMKLFTA
                     NSGNNNTRWNNPRYDQLVELAARELDSKKRVKLYDEAQKILVESDLPILPLFWTTEST
                     LLNPRFTGLEFNSMARIDLRNVRPAE"
     gene            3039..3551
                     /gene="accB"
                     /locus_tag="VF089_22030"
     CDS             3039..3551
                     /gene="accB"
                     /locus_tag="VF089_22030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX6176697.1"
                     /translation="MELSEDDVLHILKLIDESKFDYFQLEVGELKITVSKGEPIPLNS
                     SPQQVVATPPPPPAPVSAKPAAAPAAAAAAPAAQTAPASVEARAAAEGLLTVTAPLLG
                     TFYVAPEPGAPPFTHVGANVTEDTTVGLIEVMKVFNSVRAGVKGTIVEVVAQNGQFVE
                     YGQTLFLVKP"
     gene            3548..>3647
                     /locus_tag="VF089_22035"
     CDS             3548..>3647
                     /locus_tag="VF089_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6176698.1"
                     /translation="MSVSRVLVANRGEIAVRIIKACQSLGIETVAAV"
BASE COUNT          853 a         1031 c          943 g          820 t
ORIGIN      
        1 gccttcgctc atccaggcac gccgcttgat catcgaacat caccatctct actgccgaat
       61 cgtgattaga tgatggactg ttcgggaaga atggtcttca agacccgcag atagttgccg
      121 ccgagaatca gagcaatttc gttttcgtga taaccgtgct tctgcagacc atcgatcaaa
      181 ttgggcagat cctctatctc ctcgaatcct ttgatcaacg gatctttgtg cagcggatga
      241 tgacgatcct tgagcgtgcg cggcccgtca tacttgacga aatccatccc tagagccaaa
      301 tgctcgacac ccacgagatt gatcatgtaa tccatgtgct tcagcatatc ctcgacaccg
      361 acttcgcctt ggccgacccg ttccggcaac gccagaatgc ctaccaggcc gccctgctcg
      421 gcaatcgctt tgatttgatc gtcggcgaga tttcttacgc tgtcgagcat gccgcaggcg
      481 ttggcgtgac tgacgatcag cggcgcggtg gcgacctcga cgctctggaa aaagccctcg
      541 cgattcatgt gcgacaaatc gacgatcatg cccagacgat tggcctcttt aatcacctcg
      601 acgccaaagt tcgtgagccc tccgccggtt cgattctccc agacgccgtc accaagctcg
      661 ttgcggaaat tccacaccgg ctgcagggag cgcagcccca gacgatagaa gttcctgaga
      721 ttttggatgc tgccgcgcaa aggcatgcaa ccttcgaagt ggagcaaaaa ttttatcgtg
      781 ccgggctcga ccttctccgg cagatcgccg cgagttctga ccatcgagat catgccctcg
      841 gatttgggcg cctcctgcaa aaactgatcg atgttttcga gcgccgtttc cagatagcga
      901 ccggtatttt gtgaatggga ataagaatcg ccactgatag cgtaaacgga gagattgatg
      961 ccgtcgcgaa tcaatcgagg cgcaatcgca tcgcggatcg gcatcggttc gccgatggaa
     1021 gtcctgtaga gctgctcata aacgtcccga tgtccttcga ccacgacgtg gctttgcaga
     1081 agctctttcc cgtccataga ttctcccatc ttttcttgaa gatctgcgcg ccttcggata
     1141 gtaatagttg cattatttat tagcaagtca cgttgaattt cgttatcaga aaaccgcgat
     1201 tgctctcctc tgctgtcact ctggcgctgc tgctgatttt cgtctcttgc aagacggcgc
     1261 cggactccga ttcgagtgct tccgccaatc attttcgcgt caatctcggc accgagccgc
     1321 cgagcttgga ctggtcgtta gccacggatg gcgtttcgtt taacgtcatc gccaatttaa
     1381 tggtgggttt gacggaattt actcgagatc tcaagccggc gccggtgatc gctaaatcct
     1441 gggatatcgc cgatggcggt aagcggatca tctttcatct gcgtgatgat gtcttgtgga
     1501 gtgacggtca aaaagtccgc gctcgagatt tcgagtattc ttggaagaga cttctcaatc
     1561 ctaaaaccgc atcggagtat gcttacattc ttttcgacct ggtcaacgcg caggaatatc
     1621 acaacggcca gcttgcagat gccgccgaag tcggtgtcag agcgcaggat gacgtcactc
     1681 ttgaagtccg gttacgccat cccgccccgt actttctctc gctcaccact ttcgaagtca
     1741 ccttcccgca gcgtcaagat atcgttgaaa ggttcgactc gcgctggacc gatccggaac
     1801 acatcgtcac caacgggcca ttcacattgg cgtcctggaa acatgaaaac gagattcagc
     1861 tgcgcgccaa tccgaactat tttctcggca agcctgtcat cgatagagtc acgatgttga
     1921 tggtgagcga aaagaccacc gccctggcga tgtacgagca gggacaactg gactttatcg
     1981 acaaccgcag catcccgacc ttggaaaaaa agcgcatcgc caaacaacgg gggtttcata
     2041 gcgtggcgca attgcgcggc tactactatg gctttgcaac ccatcgaaaa ccgtttaacg
     2101 atccgcgcgt gaggaaggcg ttcgctatgg ccatcgatcg cgacaatttc ccgaaaattc
     2161 ttcagggcgg tgaaaaacct gcatcctctt ggattcctcc cggaatgctg gcgcacaata
     2221 caaagatcgg cgtgcatttt aatccgccgg aagcccgccg tctgctacgc gaggctggct
     2281 atcccgatgg caaggggttt ccgcaagtcg tgttggcgta caataccgaa gaagaccaca
     2341 aactcgtcgc cgaggcggtg cagagcatgt ggcagcggaa tctgggggta atcgtcaact
     2401 tagataatca ggagtggaaa gtttatttaa aaaatctgca acacgatccg ccgcacattt
     2461 ttcgtttggg atggggcgcc gactaccctg accccgacaa tttcatgaaa ctatttaccg
     2521 ccaacagcgg caacaataat acccgttgga acaatccgcg ttatgatcaa ctagtcgagc
     2581 tcgctgcccg cgagctcgat agtaaaaaac gggtgaaact ttacgacgag gcgcaaaaga
     2641 ttttggttga aagcgatctg ccgatcctgc ctctcttctg gactaccgaa agcaccctgc
     2701 tcaatccccg cttcaccggg cttgagttca actccatggc ccggatcgat ctcagaaatg
     2761 tccggcccgc ggaatagaaa ctacgaggtt caaaacgtgc aagcagctca aatcgtccaa
     2821 gtcgttatga tgatgcgtct ggaagcggag cgatagccag catctatcag ttgaacgtct
     2881 tgaaccgagc gcagcgattg gaacgtctgg aacgattgga acctgagcgc agcgactatg
     2941 ttgccatgcg gcataaaatc atgtaggtgc cattagaaac cagcgcttcc ggaatccttc
     3001 cgcagtgcaa caaaagatca ccgagaaagg tagacggcat ggaactcagc gaagatgatg
     3061 tgctgcatat tttgaaacta atcgatgaat ccaaattcga ctacttccag cttgaagtcg
     3121 gcgaactcaa aatcaccgtc agcaaagggg aaccgatccc gctgaactcg tcgccgcagc
     3181 aggtggttgc gacgccgcct ccgccgcctg ccccggttag cgcgaaacca gcagctgcgc
     3241 cggccgcggc agccgctgcg ccagcggcgc aaactgcgcc cgccagtgtg gaagcgcgcg
     3301 ctgccgccga gggactgctc acggtaacgg ccccgctcct cggcaccttt tatgtagccc
     3361 cggagccggg cgccccgccg ttcactcacg tcggcgccaa tgtcaccgag gatacaacgg
     3421 tagggctcat cgaagtaatg aaagttttca atagcgttcg cgccggcgtc aagggaacca
     3481 tcgtcgaggt agtcgcgcaa aatggtcaat tcgtcgaata cggtcagacg ctctttttag
     3541 ttaaaccatg agcgttagcc gagttctcgt tgccaatcgc ggcgaaatcg ccgtccgtat
     3601 tatcaaggcc tgccagtctt tgggaatcga aaccgttgcc gccgttt
//
LOCUS       DASUTG010000945         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1184560, whole genome shotgun sequence.
ACCESSION   DASUTG010000945 DASUTG010000000
VERSION     DASUTG010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184560"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF089_22040"
     CDS             complement(<1..548)
                     /locus_tag="VF089_22040"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6176699.1"
                     /translation="MKLADLYAQPGLTLSVEFFPPKTDKGEEALFGEIELIKSLRPAF
                     CSVTYGAGGSTREKTVDLVDRIHRECGLETMCHLTVVGQSKDDARGVLRHLQEKGLEN
                     VLALGGDPPAGMPNWQPHPDGFQHAVELVREAVSFGYFSVAVAGFPEIHPRAKSRASD
                     LGYLKEKIDAGAQAIITQLFFD"
     gene            complement(642..1049)
                     /locus_tag="VF089_22045"
     CDS             complement(642..1049)
                     /locus_tag="VF089_22045"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX6176700.1"
                     /translation="MDSLFEQLGGELKLRRVIDTFIDRVFADRMIGFFFRTADKERIK
                     EMEYQLAANFLGANIEYRGRPLDRAHARHPIMGGHFARRKQILKETLESYEVADNIKE
                     AWLKHTEDLRPLITGQAGSGCDPIDARRRAIGS"
     gene            complement(1083..1367)
                     /locus_tag="VF089_22050"
                     /pseudo
     CDS             complement(1083..1367)
                     /locus_tag="VF089_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
     gene            complement(1436..1990)
                     /gene="sufT"
                     /locus_tag="VF089_22055"
     CDS             complement(1436..1990)
                     /gene="sufT"
                     /locus_tag="VF089_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX6176701.1"
                     /translation="MQPDKPITVNRDCEVLMVPSGEKLTLASGSTVWLTQSLGGSYTV
                     MTDRGYMVRVEGKDADAIGLEPVLDGQSAQASWANDIDAVESRVWEQLRSCFDPEIPV
                     NIVELGLIYDCSVTPIENAYRASVKFALTARGCGMGEVLKRDIAAKLLAIPGVLEADV
                     ELVWDPPWSQSRISAAAKLQLGMA"
     gene            complement(2017..2472)
                     /locus_tag="VF089_22060"
     CDS             complement(2017..2472)
                     /locus_tag="VF089_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6176702.1"
                     /translation="MSELDELYQEVILDHNKSPRNFREIVDANRRSEGYNPLCGDHVI
                     VYVNLADGVIRDISFKGSGCAISKASASMMTTELKGKSEAEAEKLFDTVHKMLTGEMA
                     VEEEDVERLGKLKILSGVCKFPARVKCASLAWHTVNAALKGTDEVASTE"
     gene            complement(2472..3731)
                     /locus_tag="VF089_22065"
     CDS             complement(2472..3731)
                     /locus_tag="VF089_22065"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX6176703.1"
                     /translation="MKAVREKRLPHSGTHFDVERVRRDFPILKEQVHGKPLVYLDNGA
                     TSQKPQVVIDALNRYYKSENSNIHRGVHYLSERATASYEAAREKIRQFLNAESDHEIV
                     FVRGTTEAINLVAQSYGRTFLKAGDEIVISAMEHHSNIVPWQILCGQIGARLRVVPIN
                     HDGEFVLDEYRRLLNENTKLVSLTHVSNALGSIVPVKEAVRLAHERGVPVLLDGAQAV
                     PHFKVDVRDIGCDFYAFSGHKLFGPTGVGVLYGKSELLEAMPPYQGGGDMISLVTFEK
                     THYNVLPYKFEAGTPNIAGGIGLGVAIDFLNGFNWEEVCAHENDLLAYATDALSEIES
                     IRIIGSAKEKAGVLSFVSDHIHAHDVGTILDQEGVAIRAGHHCAMPVMQRFGVPATAR
                     ASFALYNNREDVDALVRGIHRVLKVFS"
     gene            complement(3737..4417)
                     /gene="sufD"
                     /locus_tag="VF089_22070"
     CDS             complement(3737..4417)
                     /gene="sufD"
                     /locus_tag="VF089_22070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX6176704.1"
                     /translation="MTNAVTEVVGGAGSVIDYYRLQNESSSGYLIAALEADVGRDCSF
                     TAHSVTLNGALIRNDIHVVLNGEGSECVLNGLYLPSDKQHVDNHTEIEHAKPRATSRE
                     LYKGVLSGAAHAVFNGKILVHKDAQKTDARQTNKNLLLSEHAVVNTKPQLEIHADDVK
                     CSHGSTIGQLDKDALFYLRSRGLDKAEAQSLLSYAFASDLIGRVRIAPLRARLDDYLL
                     TKFRRSQG"
BASE COUNT         1026 a         1254 c         1078 g         1068 t
ORIGIN      
        1 ttatcgaaga acaattgggt gatgatcgct tgagccccag catcaatttt ttccttcagg
       61 taacccagat cggaggcgcg gcttttagct cgcggatgaa tctcgggaaa gcctgccacg
      121 gcaaccgaga agtagccaaa agagactgcc tctcgaacca gttccacggc atgctggaaa
      181 ccatcgggat gcggctgcca attcggcatc cccgccgggg gatccccgcc caaagccagg
      241 acattttcca aacccttttc ctgcaggtgc cttaggactc cgcgagcatc atccttggac
      301 tggccaacca cggtcaaatg gcacatggtt tcgagaccgc attcgcgatg aattcgatcc
      361 acaagatcga cggttttttc gcgcgtgctt ccgccagccc cgtatgtaac agaacaaaaa
      421 gcgggtctca gcgatttgat taattcgatc tcgccgaaga gggcttcctc gcctttgtct
      481 gtttttggag gaaaaaattc aactgagagt gttaacccgg gctgggcata aaggtcagct
      541 aatttcacta aggaatattc ctgatgaata ccgtagccgg attgactatt aataacacat
      601 cgttatgaga gatcacaacc gagagaggcg agatgaaggg ttcaggagcc gattgctcgg
      661 cgccgcgcgt cgatgggatc acatccggaa ccggcctgac ctgtaataag aggccgaaga
      721 tcctcggtat gttttaacca agcctctttg atgttgtcgg caacctcata cgattccagg
      781 gtctctttca agatctgctt gcgcctcgcg aaatgacctc ccatgatcgg gtgccgggca
      841 tgcgctcggt ccaaaggccg tccccggtat tctatattcg cgcccaggaa attggccgcc
      901 agttggtact ccatctcttt aatgcgctct ttgtcggccg tgcgaaagaa aaatccgatc
      961 atgcgatcgg caaaaacccg atcgatgaat gtatcgatga ctcgtctgag tttaagctcg
     1021 ccgccgagct gttcgaaaag actgtccata ggctaacttt gattgtacat ccttgccggt
     1081 ggctacatca taccaagctg cagccgtgcc gcctcggaca tcttgcttgg atcccatggt
     1141 ggatcccata ccaaatcgat tttgacgtca cttaccccgg taatagcctt aaccttctct
     1201 tcgatttcgg ctggcataga ttgggcggcc ggacagtgag gtgaagtcag agtcatggtg
     1261 attgttaccg caccaccctc tttaacgtcg acgttgtaaa ttagccccag ctcataaata
     1321 ttaacaggaa tctcgggatc gaaacaggtt cttagggcct ccagcacttc gccttcgatg
     1381 accagctcgt taatactttc catagttcac tccaggatga ggctacgacc gctgctcatg
     1441 ccatccccaa ctgcagcttt gccgctgcag aaatgcggct ttggctccaa ggcggatccc
     1501 agaccagctc gacgtcggcc tcgagcacgc ctggtatggc gagtaacttg gctgcaatgt
     1561 cacgcttcaa aacctcaccc atgccacatc cgcgagcggt cagtgcaaat ttcacgctgg
     1621 cccgataagc gttttctata ggtgtcaccg aacagtcata gatcaatcct agctcgacga
     1681 tattaactgg gatctcaggg tcgaaacagg atcttaactg ttcccagaca cggctctcca
     1741 ctgcatcgat atcattcgcc caagacgcct gggcagattg tccatccagg acaggctcca
     1801 ggccgatagc atcagcatct tttccctcga cacgaaccat atagccccgg tcggtcatta
     1861 ccgtataact gccacccaga gactgagtta gccatactgt gctaccggaa gccaacgtca
     1921 acttttctcc gcttgggacc attagcactt cacaatcccg gttcacggtt attggtttat
     1981 caggttgcat gccatcgccc tcctatgccc gtggcatcac tctgttgaag caacctcgtc
     2041 ggttcccttc aaagcggcat tcacagtgtg ccaagccaaa ctggcgcatt tcactctcgc
     2101 tgggaatttg cacactcccg acaatatttt tagtttaccg aggcgttcca catcctcctc
     2161 ctccacagcc atctcacccg taagcatctt gtggacagtg tcaaacagtt tttccgcttc
     2221 agcctccgac ttgcccttca gctcggtggt catcattgaa gccgatgcct tggaaatggc
     2281 gcagccggat cccttgaagc tgatgtcccg aatcactccg tctgctaaat taacatagac
     2341 tatcacgtga tcgccgcaga gaggattgta gccctcggag cgacggttgg catccactat
     2401 ttcacggaaa ttgcgtggac tcttgttgtg atccaggatc acttcttggt aaagctcatc
     2461 caattctgac attaactaaa taccttcaac acccggtgga ttcctcgcac cagagcatcg
     2521 acgtcttccc gattgttgta aagagcaaac gaggcgcgcg ccgttgccgg cacgccgaat
     2581 cgctgcatca caggcatggc gcaatgatgt ccggctcgta tcgccactcc ttcttgatcg
     2641 agaatggtgc cgacgtcatg ggcatgtata tgatcggaga caaatgacag aacgccggcc
     2701 ttctctttgg ctgagccgat tatcctgatc gactctattt ccgacaaagc atcggttgcg
     2761 taagccagga ggtcgttctc gtgagcgcac acctcttccc agttaaagcc gttcagaaaa
     2821 tcaatcgcca cgcccaatcc tatcccgcct gcaatgttgg gggtaccggc ttcgaactta
     2881 tagggcagca cattgtagtg agtcttctcg aaagtcacca agctgatcat atcgccgcca
     2941 ccctgatacg gcggcatagc ctctaacagc tcggactttc catagagcac tccgaccccg
     3001 gtgggaccaa aaagcttgtg gccggaaaaa gcgtaaaaat cgcagccgat atcccgaacg
     3061 tccaccttga aatgtggcac cgcttgagca ccatccaata acactgggac gccccgctca
     3121 tgcgccagtc gaacggcttc tttcacaggc acgatgcttc ccagagcatt agagacatgt
     3181 gtcagcgata cgagcttagt gttctcgttt aacaggcggc gatattcgtc aaggacaaac
     3241 tcgccatcat gattaatggg aacgacacgc aggcgcgcac cgatttggcc gcataagatt
     3301 tgccaaggca cgatgttgga atggtgctcc atggctgaga tgacaatttc atcgccggcc
     3361 tttagaaagg ttcttccgta gctttgcgcg accagattga tggcttccgt ggtccctcgc
     3421 acgaaaacga tctcatgatc actttcagca tttaggaact ggcggatctt ttctcgcgcg
     3481 gcttcataac tcgcggttgc ccgttcactg aggtaatgaa cgccacggtg gatgtttgag
     3541 ttttccgact tgtaataacg attcagggcg tcgatgacga cctgcggctt ctgactcgta
     3601 gcgccgttat cgagataaac caacggtttg ccatgaacct gctctttgag gatcggaaaa
     3661 tctcgccgaa ccctttcaac gtcgaaatga gtgcccgaat gcggcagtcg tttttctcga
     3721 acagctttca tggttcttag ccttggctcc gtctaaactt tgtaagcaaa tagtcatcca
     3781 gccgtgcccg aagcggtgcg atgcgcaccc gtccgatgag atcgctggca aacgcgtagc
     3841 tcagaagact ctgggcctcc gctttatcaa ggccccggga cctgagataa aaaagcgcat
     3901 ccttgtcgag ctgtccaatt gtagagccgt ggctgcattt cacgtcatca gcgtggatct
     3961 ccagctgcgg cttcgtgttt accaccgcat gttcagagag taacaagttc ttattggtct
     4021 gccgagcatc ggttttctgc gcgtctttat gcacgagaat cttgccattg aacaccgcgt
     4081 gggcggcgcc gctgagaact cccttgtaaa gttcccggct tgttgcacgt ggcttggcat
     4141 gttcaatttc ggtgtgattg tcgacatgct gtttgtcaga gggcaaataa agaccattca
     4201 gcacacactc ggatccctcc ccattaagaa ccacatgaat atcgttgcgg atgagtgcgc
     4261 cgttcagagt caccgaatgg gccgtaaagc tgcaatcgcg gcccacatcc gcctccagag
     4321 cggcgatgag atatccggag ctcgattcat tttgcagacg ataataatcg atcaccgacc
     4381 ccgctccacc gactacctca gtgacggcat tagtcagagt cggctg
//
LOCUS       DASUTG010000946         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1757
            k141_1333951, whole genome shotgun sequence.
ACCESSION   DASUTG010000946 DASUTG010000000
VERSION     DASUTG010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961090
            Sequence Read Archive: SRR25158394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:12:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,417
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,346
            CDSs (with protein)               :: 4,346
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 36
            CDSs (without protein)            :: 36
            Pseudo Genes (ambiguous residues) :: 0 of 36
            Pseudo Genes (frameshifted)       :: 10 of 36
            Pseudo Genes (incomplete)         :: 24 of 36
            Pseudo Genes (internal stop)      :: 5 of 36
            Pseudo Genes (multiple problems)  :: 3 of 36
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333951"
                     /isolate="SMAG_U1757"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.07361111 N 117.1497222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VF089_22075"
     CDS             <1..1707
                     /locus_tag="VF089_22075"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEX6176705.1"
                     /translation="LPGEWRREILEGLLGADMIGFHTYEYTHHFLQCVLRILGYEHQM
                     AQLLTPDHLVKVDTFPMGIDFDKYSSAPAEPDTQQEVQALRSTLSEFKMILSVDRLDY
                     SKGILNRLQGYELFLESNPQFHGKVVLLMIVVPSRIGVVQYDRMKRQVEERVGKINGH
                     FGRVGWTPVVYQYRHVPFSSLAAFYSVSDVCLVTPLRDGMNLVAKEYVATRTDGTGVL
                     ILSEMAGSAKELPEAIIINPNNRSEIANALKEALETPIEEQKRRNKMMQRRLKRYHVT
                     RWANDFLNNLVAVREIQDRTEAKLLSPQARRAVVDQYRKSCRRALFLDYDGTLTPLVK
                     SPPMAKPDERRLALLRSLTADPANDIIITSGRDRQTLEQWFGDLRVGLVAEHGAWFRK
                     PAETWQLFKPFSVAWMRQLLPILEIYADRLPGAFVEEKENSVAWHYRSADPEQAGFLA
                     GELTDHLVSLISKTDLHVRQGSKVVEITNAGVDKGTAALKWIIEKEYDFIMAIGDDVT
                     DEDLFRVLPDWAISIRVGMVGTQAQHNLRSDAEVAELLQSFAQTSGASLKPSKSSGRS
                     TS"
     gene            1791..2141
                     /locus_tag="VF089_22080"
     CDS             1791..2141
                     /locus_tag="VF089_22080"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX6176706.1"
                     /translation="MRFEVDAPIDIDALECNCSICSKSGFLHLIVPRSRFRLLQGSAD
                     LATYTFNTGVARHLFCKTCGIKSFYIPRSNPDGYSVNVRCLDPATIRQVNVSPFDGKD
                     WEAHGADLASRSLE"
     gene            2317..>3470
                     /locus_tag="VF089_22085"
     CDS             2317..>3470
                     /locus_tag="VF089_22085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6176707.1"
                     /translation="MAYDLLIRGGRVVDGSGLPSYVADVGVKGGKIVEVGRLTGTAAR
                     TIDAGGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGVTSVVMGNCGLSLAPVKKGDE
                     DAIVKSFVRVEAIPRFALEQGVKWGWNSYGEYLDALEGKLGINVGGLVGHIAMRHNVM
                     GEEAVERTATAKEVQQMRGLVSEAMEGGALGLSTNRNERHMREDGKPVASRLADDDEL
                     FALCDVLAERNGGVIETILGRNKIEHFKFYHELAKRTQRPVLWQSLQHRWAEPNLWRE
                     QLDAVEPIFREGYQAYGLSHTVPLVRHFELKNCQVFDEFPTWKNLMFLPVEVRKQAFA
                     DGETRQKLRADLANPAPTNFHRNWNIVRVEKVVKPENEKYNGKTVAELGT"
BASE COUNT          798 a          903 c         1021 g          748 t
ORIGIN      
        1 ctgccgggag aatggcggcg cgaaattttg gagggcttgc tcggcgctga catgatcggc
       61 ttccatacgt acgagtacac gcaccatttt cttcagtgcg ttctgcgcat cctcgggtat
      121 gagcatcaga tggcccaact cctgacaccc gatcatctcg tcaaggtcga taccttcccg
      181 atgggtatcg atttcgacaa atactccagt gcgccggccg agcctgatac tcagcaggaa
      241 gtacaggcgc tgcgcagcac tctttccgag ttcaagatga tcttgtcggt cgatcggctc
      301 gactactcaa aggggatttt gaaccgtctg caaggatacg agcttttttt agagagcaat
      361 ccccagttcc acggaaaggt cgttctgttg atgattgtcg taccttcgcg cattggcgtc
      421 gtgcaatacg accggatgaa gcgacaggtc gaagaacggg tgggcaaaat caatggacat
      481 tttggacggg tcggttggac accggtggtg tatcagtacc gccatgtccc gttctcatca
      541 ctggcggcgt tctactcggt aagcgatgta tgtctcgtta caccgctccg tgacggaatg
      601 aatttagtcg ccaaggaata tgtggctaca cgaacggacg gcaccggggt cttgatcctc
      661 agcgagatgg caggctccgc caaagagttg cccgaagcaa tcatcatcaa tccaaataac
      721 cgctcggaaa tcgccaacgc ccttaaagag gcactggaaa caccaataga ggagcagaaa
      781 cggcgtaaca agatgatgca gcggcgtttg aaacggtacc atgtcacgcg ctgggccaat
      841 gattttctca acaaccttgt cgcggtacgc gaaatccagg atcgtaccga agccaagcta
      901 ctttccccgc aggcgcggcg ggcggttgtc gaccagtaca gaaagagttg ccgccgagcg
      961 ttgtttctgg actatgacgg taccttgacg ccgttggtaa aatcgccgcc aatggccaaa
     1021 ccggatgaga ggcggctggc cctgttgcgc tctcttaccg ccgacccggc taatgacatt
     1081 atcattacaa gcggacgcga tcgccagact ctggagcagt ggttcggcga tctcagggtc
     1141 ggcttggtcg ccgagcatgg cgcatggttc aggaaaccgg ccgagacgtg gcagcttttc
     1201 aaaccattct cggtggcgtg gatgcgccag ctattgccga tattggagat ctacgcagac
     1261 cggttgcctg gggcttttgt cgaggaaaaa gagaattccg tggcttggca ttatcgttcg
     1321 gcagatcccg agcaggccgg ttttctcgcc ggcgagttga cagaccattt agtgagcctg
     1381 atctcaaaaa ccgatcttca tgtcaggcag ggaagcaaag tggtggaaat caccaatgcc
     1441 ggggtcgata agggaaccgc agcgttaaaa tggatcatcg aaaaggaata cgactttatc
     1501 atggccatcg gcgacgatgt gacggacgag gatttatttc gggttctacc ggattgggca
     1561 atctctatta gagtcggcat ggtcggcacc caggcgcaac acaacctgcg cagcgacgcc
     1621 gaagtggccg aactgctgca atcattcgct caaacttcag gcgcgtcgct gaagccgagt
     1681 aaaagttcag gccgatctac gagttaagcc gcgggtccgg ggcggctctg ccgagtcacc
     1741 ttgtggagga aaatttagtt caacataggg gtgcttgcca ctgcggtcgc gtgcgcttcg
     1801 aagtcgatgc tcccatcgat atcgatgcgc tagaatgcaa ctgttcgatt tgcagcaaaa
     1861 gcggttttct ccacttgatt gtgccgcgat cccggttccg gttactccag ggatccgcag
     1921 acctggcaac ctatacattc aataccggtg tcgccaggca tctattctgt aaaacgtgcg
     1981 gcattaaatc tttctatatt cctcgttcta atcccgacgg ctatagtgtc aacgttcgct
     2041 gcctcgaccc ggcgacaata aggcaggtga acgtcagccc ctttgacggc aaagattggg
     2101 aagcacacgg agccgatctc gccagtcgat cattggagta gtgacagggt tgaagttgcc
     2161 tctggcggcc ggaggtaagg atttgctcat ggacaaaatg cggcgcaatt gatatggcct
     2221 aaatatggca agatggttca tcgttatcga accgtactag catacaacat cgcttcggat
     2281 tattgacgac tttctcggtc aactcaggag gtgcaaatgg cttatgatct gttaattcgc
     2341 ggcgggcgcg ttgtcgatgg ctcgggcttg ccgtcttacg tcgccgacgt aggtgtgaaa
     2401 ggtggcaaaa tcgtcgaagt tggacggcta accggcacag cggcgcggac gatcgacgcc
     2461 ggcggcttgg ctgtatctcc cggctttatc gaccatcata cccatctgga cgcgcagatg
     2521 ctgtgggatc cctatggcac ctgtgaaccg cagcacggcg ttaccagcgt ggtgatgggt
     2581 aactgcggct tatcgctggc gcccgtcaag aagggcgatg aagacgccat cgtaaaaagt
     2641 ttcgttcgag ttgaggcgat tccgaggttc gctctggagc aaggggtgaa gtggggatgg
     2701 aacagctacg gcgaatacct cgatgcgctg gaagggaagc tcggaatcaa tgtcggcggg
     2761 ctggtcggcc atatcgccat gcgccacaac gtcatgggtg aggaggcggt ggagcgcacg
     2821 gctaccgcca aagaagtcca gcagatgcgc ggcttggtct cggaagcaat ggaaggcggc
     2881 gccttgggat tatcgaccaa ccgcaacgag cgtcacatgc gcgaggacgg caagccggtg
     2941 gcgagtcgat tagccgacga tgatgagctg tttgcgcttt gcgatgtttt ggccgagcgc
     3001 aacggtggcg ttatcgagac gattttgggc cgcaacaaaa tcgaacattt taaattttat
     3061 catgagctgg cgaaacggac gcaacgaccg gttctctggc agagcctgca acaccgttgg
     3121 gcagagccca atctctggcg cgaacagctc gatgccgtcg agccgatttt ccgcgaaggc
     3181 tatcaagcct atggcttatc ccataccgtg ccgctcgtgc gccacttcga gctcaagaac
     3241 tgccaggttt tcgatgaatt cccgacgtgg aaaaatctca tgttcctccc ggtcgaagtg
     3301 cgtaagcagg ctttcgccga tggcgaaacc aggcagaagc tgcgggccga tctggccaat
     3361 ccggcgccga ccaactttca ccgaaactgg aacatcgtcc gggtcgagaa agtggtgaag
     3421 cccgagaacg aaaagtacaa cggcaagacg gtcgctgagc tgggaacgat
//
